LOCUS       DATAIR010000001         5831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_369306, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000001 DATAIR010000000
VERSION     DATAIR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369306"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..563
                     /locus_tag="VHM69_00005"
     CDS             84..563
                     /locus_tag="VHM69_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2738796.1"
                     /translation="MEQRDIRELRVGEIVHEEWPTLGPEETVEGAIKLFAETGISGAP
                     VVEGGRIIGIVTEGDLIFRDADIRAPGFFEILGGLIPLGSWDEYRREALKSAGVTVDE
                     VMTRELHTVSPDSTLADAATLMAEKRIKILPVTEDDELHGVITRMDILTLHVLNPRR"
     gene            573..1682
                     /gene="alr"
                     /locus_tag="VHM69_00010"
     CDS             573..1682
                     /gene="alr"
                     /locus_tag="VHM69_00010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2738797.1"
                     /translation="MDSTASGVLSRTWAEIDLGAVRHNVGTLKRRAPNSRLMAVVKAD
                     AYGHGSVEVARTAIEAGADSLAVVTVEEGAELRRAGLDVPILVFTDLLPGGLSLAEEH
                     RLAVTAHSIPSARRIAAHTALEAHLKVNTGMNRWGVEPSEVGEARKILGSRLAGVYTH
                     FASADSDDEATKRQIERFESVLSAQPLGGVLVHAANSAATLWHPGSHYDCVRPGVALY
                     GLHPAGDTGDPASEDLRPAMTLKSYVAGVRRLAPGDGVSYGLTFRAEAPTVGATVPVG
                     YAEGYRRALSGRAEALIRGLRRPLLGRVTMDACVFGVDEEVEVGDEVVLIGSQGAERV
                     TAEELGSRAGTINYEITTGVNPSRVERSYENVRGS"
     gene            1666..2013
                     /locus_tag="VHM69_00015"
     CDS             1666..2013
                     /locus_tag="VHM69_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738798.1"
                     /translation="MFEDLNWKSAARRSAVVIALYLGLLYLLSVLFPDSAFKLDNRAP
                     IVSLLINAVIFFFVFTLVYAFIERNRNRRIAEARKQQKQGKPAAGASEAGASTLRGRP
                     NPNTSRKKSRRRR"
     gene            2017..2763
                     /locus_tag="VHM69_00020"
     CDS             2017..2763
                     /locus_tag="VHM69_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2738799.1"
                     /translation="MILTTALGPPLDPAVLLDAVPELSLARELEGSPYHHLDTLDHVL
                     EVVRGVERELDEGRIEAFVKKDRVQGLRLAALLHDVAKPVTRGELEGRVLFVSHDSLG
                     ALMVRRICRRLGLSARDTDLTATLTALHLKIGFMGNERTDYTPERLALAAGPFGEELA
                     VLSWADRLAAQGPRLKPEHLERHEELCTWFLRASRGLGPHPEPDYAAIDGVLPSASDA
                     DIGYAASYLRLLVARGLDEKSALARLPLFL"
     gene            2846..3046
                     /locus_tag="VHM69_00025"
     CDS             2846..3046
                     /locus_tag="VHM69_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2738800.1"
                     /translation="MNIKRAKRDRWLLGVCGGVAHTYGWNSNMVRLATAVIAVIIPGP
                     STLLALAIYVLLGFLLPESEEF"
     gene            3051..3677
                     /locus_tag="VHM69_00030"
     CDS             3051..3677
                     /locus_tag="VHM69_00030"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2738801.1"
                     /translation="MDFAEAQKEALGCTKCGLCHSRTQVVFGEGPLNAALFIVGEAPG
                     FNEDSEGKPFKGASGMLLDRLLASLDLGREKVYLTTLVKCRPPGSPPRPPKPSEVSSC
                     RPYLVSQLAAVDPKVVVALGDLPSRVLTGRKESVSRIRGRAIPLDGRYVFPTLSLQRA
                     LYTPADRAHLVSDFSRLPELLEAERPSTYETLNVTRTPEAEPVQPGLW"
     gene            3690..4139
                     /gene="tsaE"
                     /locus_tag="VHM69_00035"
     CDS             3690..4139
                     /gene="tsaE"
                     /locus_tag="VHM69_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX2738802.1"
                     /translation="MELKGVDEAALAELAAAVAGVLEPGDVVVLSGEVGSGKTTFVRA
                     AARSLGVTDRVTSPTYQLARGYEGLKDGRRVIVNHLDLYRLEGIEDRDVLELDDYLDP
                     EAVTFIEWAEPALGVLEDPSLIHISHETQTTRRVGLGGPVAGRLTPC"
     gene            4133..4792
                     /gene="tsaB"
                     /locus_tag="VHM69_00040"
     CDS             4133..4792
                     /gene="tsaB"
                     /locus_tag="VHM69_00040"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX2738803.1"
                     /translation="MLILAFDASTAVTTVALAEGREVLAEVSVTARGASEALLPAIHD
                     ALDLAGEELGSVERVLAGVGPGTFTGIRIAAATARALSMGTGAALSKNSTLAALAAPA
                     LSCSVDVLAVLDARRGQVFARRFSEDGPTTDIYCVSPQELVVEGGPLLVGDGAVRYRD
                     ALSGLGRIPPDGSPLHRVTAAGHVISADLAPVGPEELVPIYVREPDAEVRRDLNPWSR
                     Q"
     gene            4819..5241
                     /gene="rimI"
                     /locus_tag="VHM69_00045"
     CDS             4819..5241
                     /gene="rimI"
                     /locus_tag="VHM69_00045"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX2738804.1"
                     /translation="MEAADLPSVMQIDALSLPRPWTEAIWRGELESPYGLYLVVEDRG
                     EVSGQIGVRHVLGELHITTIAVSPEHRRRGHARALIGAALAAYPKARYVHLEVRPDNA
                     AAVALYESLGFRATGRRPRYYGDDDALLMTLNLGEYHR"
     gene            complement(5207..5470)
                     /locus_tag="VHM69_00050"
     CDS             complement(5207..5470)
                     /locus_tag="VHM69_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738805.1"
                     /translation="MRTFSLLFAFIALVMLAMFLIYVVRGPVEPLIAVGLFVSFLACL
                     APAVLLLKPFEPPEELRRRREESRKEREERRRSNDGTRRGSGS"
     gene            complement(5621..>5831)
                     /locus_tag="VHM69_00055"
     CDS             complement(5621..>5831)
                     /locus_tag="VHM69_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738806.1"
                     /translation="SFLGLHRNKKATPLVEGGASVTIKPTSSTAPLLYHDLGRVHEGP
                     RKRYQVSGVAIRNLMRSGSLPLTLL"
BASE COUNT         1011 a         1892 c         1926 g         1002 t
ORIGIN      
        1 ccggtgaaga gggagtggat accataaccg gcggcgtgcc gggccgttta caatagcgtc
       61 ataacgacga tcagggggcg gacgtggagc agcgggacat tcgggagttg agggtaggcg
      121 agatcgtgca cgaggagtgg cccacgctcg ggcccgagga gacggtggag ggcgcgatca
      181 agctcttcgc agagaccggt atttcgggcg ctcccgtcgt cgagggcggg aggattatcg
      241 ggatcgtcac cgagggggat ctgatcttcc gcgacgcgga cataagggcg ccgggcttct
      301 tcgaaatcct aggcggcctc attcccttgg ggagctggga cgagtaccgt agagaagccc
      361 tcaagagcgc cggcgtgacc gtagacgagg tgatgacgag ggagctgcat acggtctctc
      421 ccgactcaac ccttgccgac gccgcgacgc taatggcgga gaagaggatc aagatcctgc
      481 cggtcacgga agacgacgag ctccacggcg tcatcacccg catggatatc ctgaccctcc
      541 acgttctgaa cccccgcagg tagagaagta ccgtggatag cacggcctcg ggagtcctga
      601 gccgtacctg ggccgagatc gacctaggag ccgtgcgcca caacgttggc accctgaaac
      661 gcagggcccc gaactcccgc ctgatggccg tcgtgaaggc cgatgcctac ggccacggct
      721 ccgtcgaggt tgcccgtacc gccatcgaag cgggagcgga ctccctcgcc gtcgtcactg
      781 tagaggaggg cgcggagctg cgccgcgccg gcctggacgt tccgatcctg gtcttcaccg
      841 acctgcttcc tggcgggctc tctcttgccg aggagcaccg tcttgccgtg acggcccact
      901 cgatcccgag cgcgcggcgc atcgcggccc acactgccct cgaagcgcac ctgaaagtga
      961 acacgggtat gaaccgctgg ggcgtcgagc cctcggaggt cggggaggcg cgcaagatac
     1021 tcggctcccg gctcgcgggc gtctacacgc atttcgcctc ggccgactcc gacgacgaag
     1081 cgacgaagcg ccagatcgag cgcttcgaat ccgtcctctc cgcgcaacct ctcggcggcg
     1141 tcctcgtgca cgccgcgaac tcggcggcca cactctggca tcccgggtcc cactacgact
     1201 gcgtccgccc cggagtggcg ctctacggac ttcaccctgc cggcgataca ggagatccag
     1261 cgtctgagga cctgcggcct gcgatgaccc tcaagagcta cgtcgccggt gttcggaggc
     1321 tcgctccggg tgacggtgtc tcatacgggc tcaccttcag ggctgaggcg cctactgtgg
     1381 gtgcgaccgt tcccgtcggg tacgcggagg ggtatcggag ggcgctctcg gggcgggctg
     1441 aggccctgat ccggggcctc aggcgcccgc ttctggggcg ggttacgatg gacgcctgcg
     1501 tcttcggcgt tgacgaagag gtcgaggttg gtgacgaggt cgtgctgatc ggctctcagg
     1561 gcgccgaacg agttacggcg gaagaactcg gctcacgtgc aggcacgata aactacgaga
     1621 tcaccacggg ggttaacccc agtcgcgtcg agaggagcta cgagaatgtt cgaggatctt
     1681 aactggaaat cagccgccag gcggtcggcg gtggtcatag cgctctacct tggcctgctc
     1741 tacctgttga gcgtgctctt cccggacagc gccttcaagc tggataaccg ggcgccgatc
     1801 gtcagcctgc tgatcaacgc ggtcatattc ttcttcgtct ttacgctcgt ctacgccttt
     1861 attgagcgca acagaaaccg gcgcatagcc gaggcccgca aacagcagaa gcaggggaag
     1921 ccggccgccg gcgcctcaga ggctggcgcc tctaccctaa gagggcgtcc gaatcccaat
     1981 acgagccgca agaagtctcg ccgcaggcgc tagcccttga tcctgacaac ggccctcggg
     2041 ccgccgctgg atcccgcggt gctcctcgac gcggtccccg agctctcgct cgcccgtgaa
     2101 ctggaaggga gcccatacca ccatctggac accctggacc acgtcctcga ggtcgtccgc
     2161 ggagtagagc gcgagctgga cgagggcagg atagaagctt ttgtcaagaa ggaccgcgta
     2221 caggggctcc gtctcgccgc cctgctgcac gacgtggcca aacccgtgac ccgcggtgag
     2281 ctcgaaggca gggtgctctt cgtctcccat gactctctgg gcgctttgat ggtccggcgt
     2341 atctgccgca gactcgggct gtcggcccgc gatacggatc tgacggccac cctcaccgct
     2401 ctgcacctga agataggctt catgggaaat gagcgcaccg actatacgcc ggagaggctc
     2461 gccctcgcgg cggggccctt cggtgaggag ctcgcggtcc tgagttgggc cgaccgcctc
     2521 gcggcccagg gaccacgcct caagcctgag caccttgagc gccacgagga actgtgcacc
     2581 tggtttctga gggcaagccg cgggctcggt ccccaccccg agcccgacta cgccgcgatc
     2641 gacggagtac tcccttccgc gtcggacgcc gacatcgggt acgcagcgag ctatctcagg
     2701 cttctcgtgg cccgcgggct cgatgagaag agcgcgctgg cgagacttcc cctgttcctc
     2761 tgatccccgg tggaagattc gggctgcggt cgtgtagaat cgcgccaaga gcgtagcggc
     2821 ctcacggggt cgaggaggta aggtcttgaa catcaagaga gcgaagaggg atcgctggct
     2881 gctcggcgtc tgcggtggcg tggcccacac ctacgggtgg aactccaaca tggtgcggct
     2941 cgccacggcg gtgatcgccg tgatcatccc cggtcccagc acgctgctgg ctttggcgat
     3001 ctacgtgttg cttggctttt tgctcccaga gagcgaggag ttctaggcgc ttggacttcg
     3061 cggaggccca gaaggaggcc ctgggctgta cgaagtgcgg gctgtgccac tcgaggacgc
     3121 aggtcgtctt cggtgagggg ccgctgaacg ccgctctgtt catagtgggt gaggcccccg
     3181 gctttaacga ggacagtgaa gggaagccgt ttaagggagc atcggggatg ctcctcgacc
     3241 ggctgctcgc gtctctggac ctggggcgcg agaaggtata cctgacgacg ctggtcaagt
     3301 gccgcccgcc gggatctcca ccacgcccgc cgaagccctc ggaggtgagc tcatgccggc
     3361 cctacctcgt ctcccagctc gccgccgtgg acccgaaggt cgtagtggcc ctcggggacc
     3421 tgccgagcag ggtgcttacg gggaggaagg agtccgtctc gcgtatcagg ggacgcgcta
     3481 taccgctgga cggccgctac gtcttcccga cgctctccct ccagcgcgcg ctctatactc
     3541 cggcggaccg tgcgcacctc gtgtcggact tctcgcggct gcccgagctc ctcgaggccg
     3601 agcgaccctc cacctacgag accctgaacg tcacccgcac tccggaagcc gaacccgtcc
     3661 agccgggact ctggtagagc acagcccccg tggaactgaa aggcgtggac gaggctgcgc
     3721 tcgcggaact ggccgccgcg gtcgccggcg ttctggagcc tggcgacgtc gtcgtgctct
     3781 cgggtgaggt gggatctggc aagaccacct tcgtgcgggc agccgcccgc tcactgggcg
     3841 tgactgacag ggtgacgagc ccgacctacc agctcgcccg cggctacgag gggctcaagg
     3901 acggtcggcg tgtgatcgtc aaccatcttg acctgtaccg cctggagggc atagaggacc
     3961 gcgacgtgct cgaactcgac gactacctcg accccgaagc cgtgacgttc atcgagtggg
     4021 ccgagccggc gctcggtgtc ctggaggacc catccctcat ccacatctcc cacgagaccc
     4081 agacaacgcg gcgcgtcggc ctcgggggtc ctgtcgcggg gcggttgacg ccgtgctgat
     4141 cctggctttc gacgcctcga ccgcggtgac gacagtagcc ctcgcagagg gccgcgaggt
     4201 cctcgccgag gtctccgtca ccgccagagg ggcctccgaa gcattgctcc cggccataca
     4261 cgatgctctg gatcttgccg gggaggagct cggatcggtg gagcgcgtgc tcgccggggt
     4321 gggaccaggg acgttcaccg gcatccgaat cgctgcggcg accgcccgcg ccctctccat
     4381 gggcacgggt gccgccctct cgaagaactc cacccttgcg gcgctcgctg ccccagcgct
     4441 atcttgctcg gtcgacgtcc tcgccgtcct cgacgccagg agggggcagg tcttcgccag
     4501 gcggttctct gaggatgggc caacgaccga tatctactgc gtcagtccgc aggagttggt
     4561 tgtggaagga ggaccgttgc ttgtcggtga cggcgccgta cgctaccgtg atgcgctctc
     4621 cggtctgggg cgcataccgc ccgacggttc gccgcttcac agagtcacgg cggcagggca
     4681 cgtcatctcg gcggacctcg ccccagttgg ccccgaagaa ctcgtcccca tctacgtccg
     4741 cgagccggac gccgaggtcc gtcgcgacct caatccctgg agccgtcagt gaaagagcct
     4801 ggaccacatt tgcgcaagat ggaggccgcg gatctcccct ccgtcatgca gatagacgcc
     4861 ctgtcgttac cgcgcccctg gaccgaggcg atctggcgcg gagagctcga gagcccctac
     4921 ggtctctatc tggtggtcga agatagaggc gaggtctcgg gccagatcgg ggtccgccac
     4981 gtcctgggcg aactgcacat tacgaccatc gccgtcagcc ccgagcaccg ccgtcgcgga
     5041 cacgcgcggg ccctgatcgg ggccgcgctc gccgcgtatc cgaaggcccg ttacgtccac
     5101 ctcgaagtgc gcccagacaa cgcggcggcc gttgccctct acgagtcgtt ggggttcagg
     5161 gcgacggggc gcaggccccg ctactacggc gatgatgacg cgctgctcat gaccctgaac
     5221 ctcggcgagt accatcgtta gatcgacggc gttcctcacg ttctttgcgt gactcctccc
     5281 tgcggcgcct cagctcctcg gggggctcga aaggtttcag cagcaggacc gcgggagcca
     5341 gacaggccag aaaagagacg aagaggccga ccgcgatcag gggttccaca ggacccctga
     5401 ccacgtagat caaaaacatc gccagcatca caagcgctat gaaggcgaag aggagcgaga
     5461 acgtccgcat aactgtcagt atagcccgtc ggttctccgc ggggtgcgcg tacggttagg
     5521 cgctgggtca tgaaaggggc cagtcttgaa tcacgttgga tacccctgac aggcccggta
     5581 acctgattgg cggggtaccg gtcgtctcct tgctaccagc tcagagcaag gttaggggca
     5641 ggcttccaga gcgcatcagg ttccttatag caactcctga aacctgatac cttttccgtg
     5701 gtccttcgtg gacacggcca agatcatggt atagtaacgg tgcagttgaa gatgtgggtt
     5761 ttatggtgac cgaagcgccg ccctcaacaa gaggagtggc ttttttattg cggtggagcc
     5821 ccaggaacga a
//
LOCUS       DATAIR010000002         5903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_93, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000002 DATAIR010000000
VERSION     DATAIR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VHM69_00060"
     CDS             <1..191
                     /locus_tag="VHM69_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2738807.1"
                     /translation="MLEENDPPVVLDVRTRSQYEQDHTRIPGSVRVSPDEVDDWARER
                     QETYGGQVEGQRVVAYCT"
     gene            complement(347..1324)
                     /locus_tag="VHM69_00065"
     CDS             complement(347..1324)
                     /locus_tag="VHM69_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003772115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEX2738808.1"
                     /translation="MIAERLGQGDEVRVVSPAISLEFIPEDQRRTARERLEGLGLRCS
                     FSRNAGVMDRFDSSPVEARVSDLHEAFADPAVKGMMTTLGGYNSNQVLAYLDYDLILD
                     NPKILSGFSDITALATAIHARTGLVTYSGPHFTTFGMRRGIEYTLEYFERCVMREEPF
                     DVPPADHWSDDPWYEDQEDRDFVPNPGYEVINEGEAEGNLLGGNLGTLCLLFGTSYMP
                     DLAGSILLLEDDEEAQPQHFDRELQSLIHQPGFEDVRAVVFGRFQRASNMDLDTMEVI
                     VRSKRELESKPVIANASFGHTTPQFTFPIGGYGRLFAAGDNVKLCIDEH"
     gene            complement(1343..2371)
                     /locus_tag="VHM69_00070"
     CDS             complement(1343..2371)
                     /locus_tag="VHM69_00070"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX2738809.1"
                     /translation="MKSGTHTFLATARVHFGADALEKLEGEARSYDRAFVITGRSLNE
                     KTDLIRRVEALLGGKHAGTFAGIGQHTPGGAVEKAAAEAEGAAADLLVSVGGGSVIDG
                     TKAVARELGYPAQVAVPTTLSGAEWAHRAGVTDESAGRKRGFADPKTVPPVVVLDPEA
                     TKYTPEKLWLSTGIRALDHAVEGFLYGGDHPVTDVTGLEGARHLMEYLPRSKQEPGDL
                     EVRLELQLAAWLSYFGPANTPMGLSHELGRRIGASYGVPHGITSCITLAPSLKIVEKR
                     FSGERWSVLAHALGGEPPERVASLVNELGLPGSLREVGVPEEDLEAIAGEYGQKAEEA
                     LEILKAAY"
     gene            2524..3192
                     /locus_tag="VHM69_00075"
     CDS             2524..3192
                     /locus_tag="VHM69_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2738810.1"
                     /translation="MERAQRQRLGTRWDLDHLGRPSVPESERSDAARNRRVILQTART
                     LFEERGVTGVTMEEIARASGVGKGTLYRRFPHKGFLCQALLDEPTRQFQQETLEVLRE
                     PGMSALEKLDDFLHGLVVFTAENLDLLYGGNEQLCGADRLERFSHPAYDWRRSTVLGL
                     LRAAVREGRVDAGLDVEYLAAALLAPLDVDFYYYQYGVQELPVERISSGLRALVPGDG
                     SSSG"
     gene            3173..4186
                     /locus_tag="VHM69_00080"
     CDS             3173..4186
                     /locus_tag="VHM69_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX2738811.1"
                     /translation="MDLQADDRRIEMELGGLHHMTAVTTDAAMNVAFYTDVLGLRLVK
                     KTVNQDDVSAYHLFYGDEVGNPGTEITFFDWPQLRVAPNHPGAGEVSTTGLRVPDRAA
                     LDWWAGRFEDLGVDHNGVEEGQIRPHLDFTDPEGQRLRLSADEGAGVPGGVPWEESPV
                     PQEFGIRGLGAVDLTVRELEPTAWVLTEVLGFRQVGERAESEGRSVTFEVGDGGPGAT
                     VRVIESRDAPWAHLGRGGVHHIAFRTPDDEEHAAWREKIRRAGIGVTPQIDRYYFRSI
                     YFREPGGVLFEIATDGPGFATDEDAAHLGEKLSLPPFLEDRREEIEANLDPIPVGGGA
                     TRR"
     gene            4241..4768
                     /locus_tag="VHM69_00085"
     CDS             4241..4768
                     /locus_tag="VHM69_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2738812.1"
                     /translation="MGNLAALLLRLVVGGLLAGHGAQKLFGSFGGPGLEGTSGFMEIL
                     GLTPGRPWAFMAGLSEFGGGVLTVLGFLNPLGPLGIIGAMAMATVKAHSGKPIWVTEG
                     GAELPVINIAAATAIILNGPGEYSLDHAFGVRLPRWLAPLGLVAIILTVMYANSEEPS
                     EQDEAREGAAGDDEA"
     gene            4765..5385
                     /locus_tag="VHM69_00090"
     CDS             4765..5385
                     /locus_tag="VHM69_00090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2738813.1"
                     /translation="MAPPDPQWLRWTKRLQAIAQDGLTYTNDAYDLGRYEQIRELAAE
                     MLAAHSTGTLEEASSLLALETGPATPKVDVRAAVFRGGRILLVKEPEGWSVPGGWADV
                     GESASEAAARETLEESGYRVRPLRLLAAHDRDRHGHPPIPYHVYKLVFLCEILDEVPS
                     SEVDTDGVGFFGEHELPELSVSRVTRAQIRRFFTQHSDPDQPADFD"
     gene            complement(5382..>5903)
                     /locus_tag="VHM69_00095"
     CDS             complement(5382..>5903)
                     /locus_tag="VHM69_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2738814.1"
                     /translation="ASGGAGHFALQLARIAGARVVALVRREEHVEMVRAAGAHEVIVS
                     EDGAAAREHAPYDLILESVGGEVLGNALSMLASGGTCVSFGVSGGTEVTFDARTFYFT
                     GGARLYGFILFHEALAKPASVGLARLVRLLDEGKLRPRIEVQAPWTEVGEIAARLIDR
                     GYRGKAVLRVGRD"
BASE COUNT         1045 a         1897 c         1913 g         1048 t
ORIGIN      
        1 tgatgctcga agaaaacgac ccgcccgtag tcctcgacgt ccgtacccgc tcgcagtacg
       61 aacaggacca tacccgcatc cccggctccg tcagggtgtc gcctgatgag gtggatgact
      121 gggcgaggga acgacaggag acatacggcg gccaggtcga gggacaacgc gtcgtcgcct
      181 actgcacctg accggatgag gctaccagcg cccgtgtggc gcgcaagcta aagaagctgg
      241 gcttcgacgc cacagcgctc gagggcggct tcgacgcctg gaaggcagag tatccaactg
      301 aagtagcgga gagagtcgga tagaccctcc gcataccgga agcgcgctag tgctcgtcaa
      361 tgcatagctt cacgttatcg ccggctgcaa ataacctgcc gtaacctccg atcgggaacg
      421 taaactgcgg cgtggtgtgt ccgaagctcg cgttggcgat gacgggcttg ctttcgagct
      481 cccgcttgct cctgacgatg acctccatcg tgtcgaggtc catgttcgaa gccctctgaa
      541 acctaccgaa cacgacggcc cgcacgtcct cgaagccggg ctgatggatc agtgactgca
      601 gctccctgtc gaagtgttga ggttgcgctt cctcatcgtc ttcgagcaag aggatcgagc
      661 cggcgaggtc aggcatatac gaggtcccga aaagaaggca gagggtcccg aggttcccgc
      721 cgagaagatt accttccgcc tctccttcgt tgatgacttc gtagccgggg ttgggtacga
      781 agtcgcggtc ctcctggtcc tcataccagg ggtcatcgct ccagtggtcc gcgggcggca
      841 cgtcgaaggg ctcctcgcgc atgacgcacc gctcgaagta ctccagcgtg tactctatac
      901 cgcgcctcat gccgaaagtg gtgaagtgcg gtcccgagta ggtaacgagg cccgtcctgg
      961 cgtgtatcgc cgtggccaga gccgtgatat ccgagaaacc ggacaggatc ttgggattat
     1021 ccaggatcag gtcgtagtcg agataggcga ggacctggtt gctgttgtag ccgccgaggg
     1081 ttgtcatcat ccctttgacc gcgggatcgg cgaaggcttc gtgcagatct gagacgcggg
     1141 cctctaccgg ggaggagtcg aaacggtcca tcaccccggc gtttctggag aacgaacagc
     1201 gcagcccgag cccttcgagc cgctccctgg ccgtgcgtct ctggtcttcg gggatgaact
     1261 cgaggctgat cgcaggcgag acgacccgaa cctcgtcacc ctgccctagc ctctctgcga
     1321 tcatgctcgc gagccgccca tcctaatacg cggctttgag gatctccaac gcttcctctg
     1381 ccttttgacc atactccccg gcgatggctt cgaggtcctc ttcaggcacc ccgacttcgc
     1441 gcaggctgcc cggcaatccg agctcgttga cgagcgatgc cacacgctcg ggcggctctc
     1501 cgccgagcgc atgcgcgagc acactccaac gctcgcccga gaagcgcttc tcgacgatct
     1561 tcaggctcgg ggcgagggtg atacacgagg ttatcccgtg cggtactccg tagctggccc
     1621 cgatcctgcg ccccagctcg tgcgagaggc ccatcggggt gttcgctggc ccgaagtacg
     1681 agagccaggc cgcgagctgt agttccagcc gcacctcaag gtcccctggt tcctgcttcg
     1741 aacgcggcag atactccatc aggtgccgcg ctccttcgag gccggttacg tcggtgacgg
     1801 gatggtcgcc accgtagaga aagccctcga ccgcgtgatc cagggccctt atccccgtcg
     1861 agagccagag cttctcgggc gtgtatttcg tcgcctcggg atctagtaca acgaccggcg
     1921 gtactgtctt cgggtcggcg aatcctcgct ttctacccgc gctctcgtcc gtaacaccgg
     1981 ccctatgggc ccactccgct cctgagaggg tcgtcggtac ggccacctgc gcaggatagc
     2041 caagctcccg cgccaccgcc ttggtcccgt ctatgacgct ccccccgccg acgctgacca
     2101 ggagatccgc cgccgccccc tcagcctccg ccgcggcctt ctccactgcc ccaccggggg
     2161 tgtgctgccc gatgcctgcg aatgtgccag cgtgttttcc gccgagcaac gcctcgacgc
     2221 gccggatgag atctgtcttc tcgttcaggc tgcgccccgt gatgacgaag gcccggtcgt
     2281 aggaccgtgc ctcgccttcg agcttctcga gagcgtcagc gccgaagtgg acccgcgcgg
     2341 tcgcgaggaa agtgtgggta ccgctcttca cggttccatg ttaccgtcat gcgctgctcc
     2401 cggcgaccgc ggcgtaatac cgaccccgaa ggatcatcgt ctcatatggt ctgactgtga
     2461 ctgaaaacgg acgctggtcc actttaaagc gcgagggcat cgcggtagac tcgatccgtc
     2521 gtgatggagc gggcacagag gcaacgcctg ggaacgcggt gggaccttga ccatctgggg
     2581 cgtccttctg tccctgagtc ggagcgcagc gacgcggcgc gcaaccggcg tgtgatcctt
     2641 cagacggcgc ggacgctctt cgaggagcgt ggcgtaaccg gcgttacgat ggaggagata
     2701 gcaagggcct cgggcgtagg gaagggcacg ctctaccggc gtttcccgca caagggcttt
     2761 ctctgccagg cgcttctaga cgagccgacg agacagttcc agcaggagac tctggaggtc
     2821 ttgcgggaac cagggatgtc tgccctggag aagctcgacg acttcctcca cgggctggtg
     2881 gtattcaccg cggagaacct ggacctcctc tatgggggca acgaacagtt gtgtggggcg
     2941 gatcggctgg agcgcttctc gcacccggcg tacgactggc gccgctcgac cgtactcggg
     3001 ctgttacggg ctgcggtccg tgagggaagg gttgacgctg ggctcgacgt cgagtacctg
     3061 gctgcggcgc ttctcgcccc gctcgacgtg gatttttact actaccagta cggggtacag
     3121 gagctaccag tggagcgcat aagtagcggg ctgcgcgcgc tggtgccagg tgatggatct
     3181 tcaagcggat gatcggagga tagagatgga actcggcggc ttgcaccaca tgacggctgt
     3241 cacgacggac gcggcgatga acgtggcgtt ctacacggac gtgctcggcc tgaggctcgt
     3301 caagaagacc gtaaaccagg acgacgtctc agcgtaccac ctcttctacg gagacgaggt
     3361 gggcaatcct gggaccgaga tcaccttctt cgactggccc cagttgcgcg tggccccgaa
     3421 ccatccgggc gccggagaag tctcgaccac cgggctgcgc gtaccagacc gcgccgcgct
     3481 cgactggtgg gccggacggt tcgaggacct cggagtggac cacaacggcg tcgaggaagg
     3541 ccagatccgc ccccacctgg acttcacgga ccctgaaggg caacgacttc gcctctccgc
     3601 ggacgagggc gcaggagtgc ccggcggtgt gccgtgggaa gagagccctg ttccgcaaga
     3661 gttcggcatc cggggcctgg gggctgtcga cctgacggtg cgggagctcg agcccaccgc
     3721 gtgggtgctc accgaggtgc tcggcttcag gcaggtgggc gagcgcgcgg agagtgaggg
     3781 ccggagcgtg acattcgagg tcggagatgg tggtccgggc gccacggtgc gggtgatcga
     3841 aagtcgtgat gcgccgtggg cccatctcgg acgcggcggc gtgcatcata tcgccttcag
     3901 gacaccggac gacgaggagc atgcggcgtg gcgggagaag atacggcgcg ccggtatcgg
     3961 ggtgacgccg cagatagacc gctactactt ccgttccatc tacttccgcg aacccggcgg
     4021 ggtgctcttc gagatagcca cggacggtcc tggcttcgcg acggacgagg acgctgctca
     4081 tctcggcgag aagctctcgc tccccccgtt cctcgaggat cgcagagagg agatagaagc
     4141 taacctcgac cccatacccg tcggaggtgg agccactcga cgttgaaccc atgtggcatc
     4201 gctgagaaat tgtccgggaa aacagacaaa ggagaacgag atgggtaacc tggcggctct
     4261 gcttctgcgg ctggtggttg gcggtctact ggcgggccac ggcgctcaga agttgttcgg
     4321 ctccttcggt ggccccggcc tggagggcac gagcggtttc atggagatac tcggtctcac
     4381 gcccgggcgg ccctgggcat ttatggcagg gctctcggag ttcgggggcg gcgtgctcac
     4441 cgtgctcggg tttctcaacc cattaggccc cttgggtatc ataggggcga tggcgatggc
     4501 gacagtcaag gcccattcgg gcaagccgat ctgggtcacc gaaggcggcg cggaactccc
     4561 cgtcatcaac atagctgccg caaccgcaat tatcctcaac ggccccggcg agtactcgct
     4621 ggatcacgcg ttcggcgtac gccttcccag gtggttggcg ccgctcgggc tggtggcaat
     4681 catactcacc gtgatgtacg cgaactccga agagccgtct gagcaggacg aggcccgcga
     4741 aggggcggcg ggggacgacg aagcatagcg cctcccgacc cgcagtggct caggtggacc
     4801 aagcgcctgc aagccatagc ccaggacggc ctcacctata cgaacgacgc ctacgacctc
     4861 ggtcgctacg agcagatcag ggagctcgcg gccgagatgc tcgcggccca ttccacgggg
     4921 accctggagg aagccagtag tttgctcgcg ctcgaaacgg gtcctgcgac accgaaggtc
     4981 gacgtgcgtg ccgccgtgtt ccgtgggggt cggatcctgc tcgtcaaaga gcctgagggc
     5041 tggtccgtgc cgggaggctg ggccgacgtg ggcgaaagtg catccgaggc cgcagcccgc
     5101 gagaccctgg aagagtcagg ctatcgcgtg cgccctctca ggctgctcgc cgcccacgac
     5161 cgcgacaggc acggacaccc gcccatcccc taccacgtct acaagctggt gtttctgtgc
     5221 gagatcctgg acgaagttcc gtcatccgaa gtagatacag acggggtcgg ctttttcggc
     5281 gaacatgagc tgccagaact ctcggtctcc cgcgttaccc gggcccagat ccgacgtttc
     5341 ttcacgcagc acagcgaccc ggaccagcct gcagacttcg attagtctcg tccgacccgg
     5401 aggacggcct tccccctata tccccggtca attagccgcg ccgcgatttc ccccacctcc
     5461 gtccacggcg cctggacttc gatgcgtggt ctgagcttgc cttcgtcgag tagtctcacg
     5521 agccgggcga gccccacgga ggcaggcttc gcgagcgcct cgtggaacag gatgaaaccg
     5581 tagagcctcg ccccacccgt gaagtagaag gtacgcgcgt cgaacgtgac ctctgtcccg
     5641 ccggagaccc cgaacgagac gcaggtcccg cctgaggcga gcatcgagag cgcgttcccc
     5701 aggacctctc cacctacgga ctccaggatc aggtcgtacg gcgcgtgctc cctcgccgca
     5761 gcaccgtctt cactgacgat gacctcgtgc gcgcctgctg cacgcaccat ttccacgtgc
     5821 tcctcgcggc gtaccagggc gacgacccgc gccccggcga tgcgggcgag ttgcagtgcg
     5881 aagtgcccgg caccgcccga tgc
//
LOCUS       DATAIR010000003         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_46330, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000003 DATAIR010000000
VERSION     DATAIR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46330"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHM69_00100"
     CDS             <1..575
                     /locus_tag="VHM69_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2738815.1"
                     /translation="KLLSWVALERAGRIARQRGLPAGEGRWIVQRDEIYEEIMEKGWN
                     PRKNSFVQYYGSDALDASLLMMPLVKFVGPTDPRWIATLQRIQEELAYDTLVDRYKTE
                     EAAPDGLEGSEGSFSLCSFWLVECLTQAGRLDEARLALEKMFSYANHLGLYAEEIGQS
                     GEALGNFPQAFTHLALISAAVHLDRALGAD"
     gene            582..1484
                     /gene="ftcD"
                     /locus_tag="VHM69_00105"
     CDS             582..1484
                     /gene="ftcD"
                     /locus_tag="VHM69_00105"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX2738816.1"
                     /translation="MQAPLFEAVPNFSEGRDHARISRITDVVRAVPGVRVLGLHSDPD
                     HNRSVLTFAGEEDAVLSGAVALAKACAAELDLSTQAGEHPRMGVLDVLPVVPLEGATM
                     EDAVRLAHRIGEGLGSLGFPVFLYGHAATAPHRHKLPEIRRGGYDGVVSRLEDPQWSP
                     DYGPKVPDPHTGAVAVGARPFLVAFNAFLDTGDVEVARNVAGQVRESGGGLRAVRALG
                     FSVGGKAQVSMNLTDLAETPIPVALEAVRTAAAEHGATVESTELVGLAPLEAILQTAR
                     HYLGLRELGPDHILETRLYGVSET"
     gene            1518..1988
                     /locus_tag="VHM69_00110"
     CDS             1518..1988
                     /locus_tag="VHM69_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX2738817.1"
                     /translation="MLIYFAGPLFSEAERRFNLELAERLEALGFDVFLPQRDGAEREK
                     PPYDAMTPEERRRAMFDLDKSRILDSDIFVFVLDGRVPDEGACVELGIAYCQRELQAS
                     EKLLIGLHTDPRAAFIGARLNPMVSVPLDCVADDEEMLLRILDEHMIMEEGGTR"
     gene            2057..2581
                     /locus_tag="VHM69_00115"
     CDS             2057..2581
                     /locus_tag="VHM69_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738818.1"
                     /translation="MGAQFIFHGKRDYHAAARYVRRLPYGRNADRSDYGLVLLESRGT
                     CSTKHALLAALAREHDRPVELRLGIYVMDGDNTPGVGQVLRRHGLDGLPEAHCYLAYR
                     GLRIDLTHAEPAETVVDFLHEETIEPEGIGVYKVDTHRRFVREWALQRDLDFDEIWRI
                     REECILALSERDRT"
     gene            2601..3425
                     /locus_tag="VHM69_00120"
     CDS             2601..3425
                     /locus_tag="VHM69_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2738819.1"
                     /translation="MCGREITVAAIQMSSTPDKDENLETAERLIRAASSSGAGLVALP
                     ELWICHGLEKVYRENAEPIPGPTTDFLGGLARELGLYLLGGSILETDPRSTKLHNTST
                     FFGPDGEMSAVYRKLHLFDVKAPDREYLESETIGPGTEIVTAKAGAAKLGLSVCYDIR
                     FPELYRLLALRGAEVFAVPAAFTLQTGKDHWELLLRARAVENQAFVIAPAQWGQKADG
                     RWTYGRSMVVDPWSTVLATCPDRDGYALATLDLDYLDRFREEFPALKNRRPESYAW"
     gene            3455..3961
                     /locus_tag="VHM69_00125"
     CDS             3455..3961
                     /locus_tag="VHM69_00125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2738820.1"
                     /translation="MKVEIRDAKISDATAIADLFYHTVLNVNVRDYSGAQVEAWAGPA
                     PDPGKWEKRIAAGEGMKRTFVAHQGERVLGFSEFEGDGHIDTLYAHHEFQGCGIASRL
                     LDRIEAEAQRLGLARLYTEASITAKPFFRKRGFSVVTPQMVEVRGQTFRNYRMEKTGH
                     TCESDEDA"
     gene            3958..4704
                     /locus_tag="VHM69_00130"
     CDS             3958..4704
                     /locus_tag="VHM69_00130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2738821.1"
                     /translation="MNYEDLKRISDSVGERSGWNFSRVREERDHVPWEFVEVVRRYLH
                     PEDRVLDIGTGGGETFLGLSTRFGTGMGIDFDPEMIRVARENAQKQSASHVSFEPMDA
                     RCLRFSEGSFDVVLNRHAPTEVAEIVRVLRPGGVFITQQVGRRNAQNICSVFGFDPNV
                     EWPEDFHDVHLRARAFSEAGCEVVAYGEYDVGYRFLDIESLIFWLKAVPMPFDPDLHW
                     RQVRQVIARYGNSDVIETNEHRELLIVRKR"
     gene            complement(4747..>5602)
                     /locus_tag="VHM69_00135"
     CDS             complement(4747..>5602)
                     /locus_tag="VHM69_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX2738822.1"
                     /translation="SLPLFPVYGVAAVLLAGGWAGVRRRGAEAVLLGLVAGFGTLLTS
                     LYVVPELSGALGGLAASALFLARRRRRLTGIPVRALLPYAFLLALLTAVNGIGVLRSA
                     IEELGPAFEGPGLPLLVSGAFAALLLGLGRDDAGAATRRTVTQWLPTAGAVLTFVLAG
                     EVVAGSGAAGLLAGGAEGLGSFFPAVSPVLAALGGALTGSNAASNALFMPLQVEAARG
                     LDVSTGLTAAVQNVAGSHASMLAPQRLVLAATATGLLGREGDILRAAAAPVAVSIAIL
                     AILGMFLS"
BASE COUNT         1123 a         1747 c         1795 g          937 t
ORIGIN      
        1 ccaagctcct gagctgggtc gccctcgaga gggcgggtag gatcgcccgc cagcgcgggc
       61 ttcccgccgg ggaaggacgc tggatagtcc agcgagacga gatctacgag gagatcatgg
      121 agaagggctg gaaccccagg aagaatagct tcgtccagta ttacggttcc gacgccctcg
      181 acgcctcgct tctcatgatg cccctcgtca agttcgtcgg cccgaccgac ccgcgttgga
      241 tcgccaccct gcaacgaatc caggaggagc tggcctacga cacactcgtc gaccgctaca
      301 agacggagga agccgccccg gacggcctgg aagggagcga gggcagcttc agcctgtgct
      361 ctttctggct cgtggaatgc cttacgcaag caggtcgcct ggacgaagcc cgcctggccc
      421 tggagaagat gttctcctac gccaaccacc tcgggcttta cgccgaggag atcgggcagt
      481 ccggcgaggc tcttggcaac ttcccccagg cttttaccca cctggccctg atcagcgccg
      541 cagtccacct ggatcgcgcg ctgggagccg actgaaccgc cgtgcaagct ccgctcttcg
      601 aggccgtccc gaacttcagc gagggaaggg accatgccag gatctcacgc atcaccgacg
      661 tagtccgcgc agtccccggc gtgcgcgtcc tcggcctcca ctcggacccc gaccataaca
      721 ggagcgtact tactttcgca ggcgaggagg acgccgtgct ctcgggcgcc gtcgccctcg
      781 ccaaggcgtg cgccgccgaa ctggacctct cgacccaggc aggtgagcac ccgcggatgg
      841 gtgtgctcga cgtactcccg gtcgtgcccc tggaaggcgc aacgatggag gacgctgtga
      901 ggctggcaca caggatcggc gagggcctcg gctcacttgg attcccagtc ttcctctacg
      961 ggcacgcagc gaccgcgcca caccgccaca agctaccgga gatcaggcgt ggcggctacg
     1021 atggcgtcgt ctcaaggctc gaagaccccc agtggagtcc ggactacggg ccgaaggtgc
     1081 cggaccctca cacaggagcc gtcgccgtcg gcgcccgtcc tttcctcgtg gcattcaacg
     1141 ccttccttga caccggggat gttgaggtcg ctcggaacgt ggccgggcag gtccgggaga
     1201 gcggcggtgg cctcagggct gtgagggcac ttggcttcag cgtgggcgga aaggcccagg
     1261 tctccatgaa cctgaccgat ctcgcagaga cccctatccc tgtggccctg gaagccgttc
     1321 gcacagcggc ggcggaacac ggcgccacgg tggagtccac cgagctcgtc ggacttgcgc
     1381 cgctcgaagc gatcctgcag accgccagac actaccttgg cctgcgcgag ctggggccgg
     1441 atcacatcct cgagacgagg ctgtatggcg tatccgagac ttgaatcgca gcgagctgta
     1501 ttccggatgg gttacgcgtg ctgatctact tcgcaggacc actgttttcg gaggcggagc
     1561 ggcgcttcaa cctcgaactg gccgagagac ttgaggcact cggcttcgac gtcttcctcc
     1621 cgcagcgcga cggggccgag cgcgagaaac cgccctacga cgcgatgacg ccagaggaac
     1681 ggcgccgcgc catgttcgac ctcgacaaat ccaggatcct ggactccgac atcttcgtgt
     1741 tcgtgctcga tggccgcgtc cccgacgaag gcgcgtgtgt ggagctcggt atcgcctact
     1801 gccagcgaga gcttcaggcc agcgagaaac tactcatcgg actacacact gatccccgcg
     1861 ccgcctttat cggtgcgaga ctcaacccga tggtgagcgt cccgctggac tgtgtggccg
     1921 atgacgagga gatgctgcta cgaatactcg atgaacacat gatcatggag gaaggcggta
     1981 cgagatgacg tccggaacgg aacgccttgg gagtttggag aaattaccga acttcgatct
     2041 cgagcacgga gccaggttgg gcgcacaatt tatcttccat ggcaagcggg actatcatgc
     2101 ggctgcgcgt tacgtccggc gactacccta cggcagaaac gcagaccggt ccgattacgg
     2161 gctggtactt ctcgaaagcc gcggcacgtg cagcaccaaa cacgcgctcc tggcggcgct
     2221 cgcgcgggag cacgacaggc ccgtggaact ccggctcggc atctacgtga tggatggtga
     2281 caacacaccg ggcgttggcc aggtcctccg ccggcacggg ctcgacggct taccggaagc
     2341 gcactgctac ctcgcatatc gcggacttcg catagacctt acccatgctg aaccggccga
     2401 aactgtcgtc gacttccttc acgaagagac catcgaaccg gaagggatcg gcgtgtacaa
     2461 ggtagacacg cacaggaggt ttgtgaggga atgggcgttg cagcgcgacc tggatttcga
     2521 tgaaatctgg cggatcaggg aagagtgcat cctcgcgctc tcagaacgag acaggactta
     2581 aggcagaaag gaacgacgca atgtgcggac gcgagataac ggtagcggcg atccagatgt
     2641 cgtcgacccc agataaggac gagaacctgg agacggcgga acgtctgatc cgcgcggcct
     2701 cctcctcagg cgccgggctg gtggcgcttc ctgaactgtg gatctgccac ggactggaga
     2761 aggtctacag ggagaacgcc gaacctatac cagggccgac gaccgatttc ctgggcggcc
     2821 tcgcacgcga actcggactc tacttgctcg gaggatctat cctcgaaaca gacccacgct
     2881 caacaaaact gcataataca tcgacgttct tcgggcctga cggtgagatg tcggcggtct
     2941 accgcaagtt acacctcttc gacgtgaagg cccctgaccg cgaatacctg gagagcgaga
     3001 cgatcgggcc cggtacggag atagtgaccg cgaaggccgg cgcggcgaag ctcggactct
     3061 ccgtctgcta cgacatcagg ttcccggaac tctaccgtct cctggcactg cgcggcgcgg
     3121 aggtgttcgc ggtccccgcc gccttcacgc tccagacagg caaggaccat tgggagcttc
     3181 tgttaagggc gcgcgccgtc gagaaccagg ctttcgtaat tgctcctgcg cagtggggcc
     3241 agaaggctga tggccgctgg acatacggac ggtccatggt cgtggacccg tggagcacgg
     3301 tcctcgcgac gtgtccagac cgcgacgggt acgcgctcgc caccctcgac ctcgactacc
     3361 tagaccgctt ccgcgaagag tttccggccc tgaagaaccg ccgtccggaa tcttacgcct
     3421 ggtaagctca gacatgccat tcggtcagag gttcgtgaaa gtcgagatcc gggacgccaa
     3481 gatcagcgac gccacggcta tcgccgatct cttctaccac acggtcttga acgtgaacgt
     3541 cagggactac tccggggcac aggtcgaagc gtgggccggg ccggcaccag atccagggaa
     3601 gtgggagaag cgcatcgccg cgggggaagg aatgaagcgg acgttcgtag cacaccaggg
     3661 cgagcgggtg ctcggctttt ccgagttcga gggtgatggt cacatcgaca cgctctacgc
     3721 acatcacgag tttcagggat gcgggatcgc gtcgcgcctg ttggacagga tcgaagccga
     3781 agcgcaacgt ctcggactcg cgcgtctgta cactgaagcg agcatcaccg ccaaaccgtt
     3841 cttccgaaag aggggtttct cggttgtaac accgcagatg gtcgaggttc gcggccagac
     3901 ttttcgaaac taccgaatgg agaaaacagg acatacttgc gagtcggacg aggacgcatg
     3961 aactacgagg acctcaaacg gatctccgat tctgtgggcg aacggtcggg ttggaacttc
     4021 tcacgggtga gggaagagcg tgaccatgtg ccctgggagt tcgttgaggt cgtgcgacgc
     4081 tatctgcatc ctgaagacag ggtactcgac atcggaaccg gcggagggga gacttttctc
     4141 gggctttcga cgaggttcgg tacaggcatg gggatagatt ttgatcccga aatgatccgt
     4201 gttgccaggg aaaacgcgca aaagcagtct gcttcgcacg tctccttcga gccgatggat
     4261 gcgcggtgct tgaggttctc ggaaggatct ttcgacgtcg tgctaaaccg tcacgcccca
     4321 acagaggtgg ccgagatcgt ccgggttctc cggcccggtg gcgtgttcat aacgcaacag
     4381 gtgggacgga gaaatgctca gaacatctgc tcggtattcg gattcgatcc gaacgtcgag
     4441 tggcccgagg atttccacga tgtgcatctc agggcgcgag ctttctcgga ggctggatgc
     4501 gaggtggtgg cgtacgggga gtacgacgta ggttaccgtt ttctcgacat agagtcgctc
     4561 attttctggc tcaaggctgt cccgatgcct tttgatcctg accttcactg gcggcaggtc
     4621 cggcaggtga tcgctcgcta cggcaactct gatgttatcg agacgaacga gcaccgcgag
     4681 ttgctgatcg tacggaagcg gtaagccgct cgctgaatcg gacacttacc gatgcaaaaa
     4741 gtgaacctaa ctcaggaaca tcccgaggat agcgagtatg gcgatggaga cggcaacagg
     4801 tgctgcggcc gcacgcaaga tgtcgccttc gcggccgagc aagccagtag ctgtggccgc
     4861 gagtaccagg cgctgcgggg cgagcatgct cgcgtggctc cccgcgacgt tctgcaccgc
     4921 agccgtgagc cccgtcgaga cgtcgagccc acgagccgcc tcgacttgga gcggcatgaa
     4981 aagcgcgttc gaggcggcgt tcgaccccgt gagcgcaccg cccagggccg cgagcacggg
     5041 cgagaccgca gggaagaagc tacccagacc ttccgcgcct ccagcaagga gccccgccgc
     5101 cccgctcccc gccactacct cgcccgccag gacgaacgtc aggacggccc ctgccgtcgg
     5161 gagccactgc gtcacggttc tccgggtcgc agcgcctgcg tcatccctgc cgagaccgag
     5221 cagcaacgcc gcgaacgccc ctgacaccag cagagggagg ccaggacctt cgaaggccgg
     5281 accgagctcc tcgatggccg accgcagcac tccaatgccg ttcaccgccg tgagcagcgc
     5341 gagcaggaac gcgtagggga gaagcgcgcg caccggtatc ccggtgagcc gccggcgtct
     5401 tcgtgccaga aacagcgctg aagccgcgag tccgccgagg gcaccggaga gctcagggac
     5461 cacgtacagg ctcgtaagga gagtcccgaa gccggcaaca aggccgagca ggaccgcctc
     5521 cgcgccgcgt cgcctcacgc ccgcccatcc gccggcgaga aggacggccg cgacgccgta
     5581 taccgggaag agtggaaggc ta
//
LOCUS       DATAIR010000004         4155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_184820, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000004 DATAIR010000000
VERSION     DATAIR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184820"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHM69_00140"
     CDS             <1..230
                     /locus_tag="VHM69_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738823.1"
                     /translation="NEEDQKATVTELATVFNELLPVIPLWERYGNNPALDGERVTGWL
                     PEGDPIYENSPYEDSFTVIMLMDGTLKPAGQ"
     gene            329..1495
                     /locus_tag="VHM69_00145"
     CDS             329..1495
                     /locus_tag="VHM69_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2738824.1"
                     /translation="MTRLAVIGLGHRAAGMVSAMQAVDPGVRIGAVADPDEEGVRRRM
                     VGQDSARIYRSADAMLEDAADYDGVIIGTRCHLHTEMAIKVAAVGLPLFLEKPVSISL
                     DELESLREAYRGREDEVVVSFPLRYMPIFSKVMEIVKSGRLGTINQIQAFNNVPYGGV
                     YFGQWYRNYDQTGGLWLQKATHDFDCINLLAGSRPVGIAAMESRRVYGGEMPHDLRCS
                     ACDITETCPESPVNIARRGDDGGMYQDSDDKTDHWCAFSREIRSHDAGSALIMYENGI
                     HASYSQNMLSRRSAYRRGATVVGYEATLDFDLASMDIRVIHHHSSEVEHMHIDTGSDG
                     HGGGDHILAESFLDLIAGRASPNASLRDGLLSAAMCLAARESARTKTFQQIQSA"
     gene            1630..3735
                     /locus_tag="VHM69_00150"
     CDS             1630..3735
                     /locus_tag="VHM69_00150"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX2738825.1"
                     /translation="MRLIISEKANTAKKIAQFLSERSVKEGKHRSVPHHTFTWKGEET
                     VSVGLKGHVLNPEYPEEYSNWQKVEPRELIDAEILKSVSEKGVAQAITSLAKKADRVV
                     IATDFDREGELIGVEALSLVFEANPKLMDHVERARFSALTPGEVGRAFDELLEVSNAL
                     ADAGEARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLVLIAERERERRAFV
                     PEPYWELEVNLRNGEPFTVRHQTERFKDEAKAKRSYENVADTATVVEVQQKSATRKPP
                     TPFNTTGFLTAAANLGISPSRAARIAEDLYTDGYISYPRTDNTVYPRSLDLREVLDYL
                     KRVEGAGQHAEKLLSQDKLSPTRGKKETTDHPPIYPTGYASKESMRDDRWKIYQLVVR
                     RFLATLSGPAKTLRTTLRFDSGGEPLVTGGTVVTEEGWLGVYPYSRRADEEIPNLSEG
                     DVVQVIGKEIFSKETQPPGRYGAGRLITVMEELGLGTKATRPNIIQNLYDRGYVHDDP
                     LVPTEKGIAVAQALTDFASEIASHEMTAELERSMDEISEGKISKESVVDSSRDVLRRV
                     YDHLQSSETEFADIVWEGIRTDETLGKCPESGHDLIIRRNRKSRKRFVGCTGYPDCRV
                     TYPLPQRGEIIPLGTRCDACGSPEIKVLGGKRPWVTCINMDCPKKQEQREAAAKAAEQ
                     EGGEGSSTQSAQELQKSTT"
     gene            3902..>4155
                     /locus_tag="VHM69_00155"
     CDS             3902..>4155
                     /locus_tag="VHM69_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2738826.1"
                     /translation="MAPIIGITATLKEDVESVAERPLGRFVRADLDYIDGVVGAGGVP
                     VVLPPVGDERAAEAVIHGLDGLLLSGGSDLDPAYYDEEPLP"
BASE COUNT          867 a         1258 c         1325 g          705 t
ORIGIN      
        1 tcaacgagga ggatcagaaa gcgaccgtga ccgagctggc cacggtcttc aatgagcttc
       61 tgcctgtgat cccgctgtgg gagcgttacg gtaacaaccc cgcgctcgac ggtgaacggg
      121 ttacgggctg gctgcccgaa ggcgacccca tttacgaaaa cagcccctac gaggacagct
      181 tcacggtcat aatgctcatg gacggcacgc tcaaacccgc cgggcagtag tccggagatg
      241 ttgtgggagg ggccctggtc tctcccactt tccgcggcaa gcgtcgggcg ggccacgacg
      301 cgcggctgtt agatccgaga ggagaggtgt gacgagactt gcggtgatcg ggctcggaca
      361 cagggcagcc gggatggtca gtgcgatgca ggcagtcgac cctggcgtga ggatcggtgc
      421 cgtcgcagac cctgacgagg agggcgtgcg ccggcggatg gtggggcaag attcggcgcg
      481 aatataccgt tcggccgacg ccatgctcga agacgcggcg gactacgacg gcgtcattat
      541 cgggacgagg tgccaccttc acaccgagat ggcgatcaaa gtggccgccg ttggccttcc
      601 gctctttctt gagaagccgg tttcaatcag cctggacgag ttggagtcgt tgcgggaagc
      661 ttatcggggg cgggaggatg aggtggtggt ttcgttcccc ttgcggtaca tgcccatctt
      721 ctcgaaggtg atggagatcg tgaagtcggg cagacttggc accatcaacc agatccaggc
      781 cttcaacaac gtgccttatg gcggggtgta cttcggccag tggtaccgta actacgacca
      841 gacgggcgga ctctggctcc agaaagccac ccacgatttc gactgcatca acctcctcgc
      901 aggaagccgt cccgtaggaa ttgccgcgat ggagtcccgc agggtctacg gtggagagat
      961 gccccacgac ctcaggtgct cggcctgcga catcaccgag acgtgccccg agagccccgt
     1021 gaacatcgcc cgccgtgggg acgacggtgg gatgtaccag gacagcgacg acaagaccga
     1081 ccactggtgc gctttcagcc gcgagatacg gagccacgac gcaggatcag cgctcatcat
     1141 gtacgagaac ggtatccacg ccagctacag ccagaatatg ctgtcgcgcc gctccgcgta
     1201 caggcgcggg gcaacggtag tcggctacga ggcaaccctc gatttcgacc tggcctcgat
     1261 ggacattaga gtcatccatc accactccag tgaggtcgaa catatgcaca tcgacacggg
     1321 gagcgacggg cacggaggcg gggatcacat cctcgccgag agctttctcg accttatcgc
     1381 gggacgcgcg agcccgaatg cctccctcag ggacgggctt ctgagcgccg ctatgtgcct
     1441 cgccgcccgc gagtcagccc ggacaaagac gtttcagcag atacagagcg catagataga
     1501 ccgtcccgtc cacggggact tgagcataca ggcttcagcc ccggcggacg tcgagaaccg
     1561 gttctccggg ggtcttccgg cccgcggggt cgaagcaatc cgctgcattg tgttataacc
     1621 aggatcaata tgcgactaat tatttccgag aaggcgaata ctgcgaagaa gatcgcccag
     1681 ttcctctctg agcggtctgt caaggagggc aagcaccgct ccgtgccgca ccacacgttc
     1741 acgtggaagg gggaggagac ggtctctgtc ggcctgaaag ggcacgtcct caaccccgaa
     1801 tatcccgagg agtactcgaa ctggcagaag gtcgagccgc gggagttgat agacgcggag
     1861 atcctgaagt ccgtctccga aaagggcgtc gcacaggcca taacctcgct cgcgaagaag
     1921 gccgacaggg tcgtaatcgc cacggacttc gaccgcgagg gggagttgat cggggttgag
     1981 gccctctccc tggtcttcga ggccaacccg aagctgatgg accacgtcga acgagcccgt
     2041 ttctccgccc tcacgcctgg cgaggtaggc cgcgccttcg acgagctact ggaggtctcc
     2101 aacgccctgg ccgacgccgg tgaagcgcgg caggatatag atctgatctg gggcgccacc
     2161 ctgacccgct gggtatcccg agccacgaag cgctacggga gcgccttcct ctccgtcgga
     2221 cgggtccagt caccgaccct cgtcctgata gcggagcgcg agcgggagcg gcgggctttc
     2281 gtccccgaac cgtactggga gctggaggtg aacctgagga acggcgagcc gttcacggtc
     2341 cgccaccaga ccgagcgctt taaagatgag gcgaaggcca agagatccta cgagaacgtc
     2401 gcagacaccg cgacggtagt cgaagtccag cagaagtcgg cgacacgcaa accgccgacg
     2461 ccgttcaaca ccacaggttt cctgactgcg gccgcgaacc tcgggatcag cccttcaagg
     2521 gccgcccgca tcgcagagga cctttacacc gacggctata tctcgtaccc gaggacggac
     2581 aacaccgtct acccgcgctc gctcgacctg cgcgaggtac ttgactatct caagcgtgtc
     2641 gagggtgccg ggcagcacgc cgagaagctg ctctcccagg acaagctatc gccgacgcgg
     2701 ggaaagaagg agacgacgga ccaccccccg atctacccta cgggctacgc ctcgaaggag
     2761 tcgatgcggg acgaccggtg gaagatctac cagctcgtcg ttcgtcgctt cctcgcaacc
     2821 ctctccggcc cggcgaagac gctgcgcacg acgctccgct tcgactccgg cggcgagccg
     2881 ctcgtcacgg gcggtacggt agtcacggag gagggctggc tcggcgtcta tccatatagc
     2941 cggcgcgcgg acgaggagat ccccaacctc tcagaaggcg acgtggttca ggtgatcggg
     3001 aaggagatct tctctaagga gacccagcca cctggacgct acggcgctgg ccgcctcata
     3061 accgtgatgg aggaactcgg tctgggcacg aaggcgacgc gcccgaacat catccagaac
     3121 ctctacgacc gcggctacgt acacgacgac cctctcgtcc cgacagagaa gggcatcgcc
     3181 gtggcgcagg ccctcacgga cttcgcatcg gagatcgcct ctcacgagat gaccgccgaa
     3241 ctggagcgga gcatggatga gatctccgag ggcaagatca gtaaagagtc cgtggtcgac
     3301 tcctcgcgcg acgttttgcg ccgcgtctac gaccatctcc agagctcgga gaccgagttc
     3361 gcggatatcg tgtgggaggg cattcggacc gacgagactt taggtaagtg ccccgaaagc
     3421 ggtcacgacc tcataatccg ccgcaaccgt aagagccgca agcgcttcgt cggctgcaca
     3481 ggctaccccg attgccgtgt cacctatcct ttgccgcagc gcggtgagat aatacccctc
     3541 ggcaccaggt gcgacgcctg cggctcgccc gagataaagg tgcttggggg gaagcgtccc
     3601 tgggtaacat gcatcaacat ggattgcccg aagaagcagg agcagcgtga ggccgccgcg
     3661 aaagccgcgg aacaggaggg gggtgagggc tcatcgacac agagcgctca ggaacttcag
     3721 aagtccacaa catagagagc aggctcgacc tcgagctcgc ggatgaacaa cctttctacg
     3781 aaccctcact ctatatccgc gccagcgggt acctcatcct tctcgctatc gtcgccgtaa
     3841 tctggtactc tgccgtatat agttaagata attgaagata ttgttaaagg gtgtgtagaa
     3901 cttggcgccg atcatcggca taacggcgac gttaaaagaa gatgtggaat cggtagcgga
     3961 gcggcctttg ggcaggttcg tgagggctga tctggattac atcgatggcg ttgtcggggc
     4021 tgggggcgta cctgtcgtat tgccgcccgt cggggacgag agggctgctg aggcggtcat
     4081 tcacgggctc gacggcctgc tattgagtgg cgggagcgac ctggacccgg cctactatga
     4141 tgaggaaccc ttgcc
//
LOCUS       DATAIR010000005         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_230934, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000005 DATAIR010000000
VERSION     DATAIR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230934"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..982)
                     /locus_tag="VHM69_00160"
     CDS             complement(221..982)
                     /locus_tag="VHM69_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2738827.1"
                     /translation="MGYSVGQVADLAGVTIRTLHHYDEVGLLKPGGRSAAGYRVYEAL
                     DLERLQRILFYRELGFTLKEISTIVDDPRTDADGHLRRQRALLTDRIERLRAMVEAID
                     YETEARTMDIPLTPEERLEVFGEFRPEDYAEEAKQRWGETEAYRQSQRRVASYKKEDW
                     QQLKAEEEEVRARLAAALAAGLAPDSEEAMDAVEAHRQHISRWFYECTHDIHLGLTEM
                     YVNDERFRSNYDATAPGLARYIRDAAHANARRADR"
     gene            1154..>1965
                     /locus_tag="VHM69_00165"
     CDS             1154..>1965
                     /locus_tag="VHM69_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2738828.1"
                     /translation="MRYGLSLPNFGEGIDARTIAGLAREAEEAGWDGFFIWDHLLAFS
                     PRPVPVVDPWISLAAAAMSTSRIRLGPMVTPLPRRRPAKLARESASLDHLSGGRMILG
                     VGIGAMPYEWDYLGEEADMRVRGAMLDEGLDVLTGLWTGQPFAHRGKHYRIQGESPDE
                     DWRAVFYPPPLQRPRVPIWVAGTWPVKPPFRRAARWDGVFPMKAEGRRILPMTPEDVR
                     HVKRYVAEHRTEEGPFDVVVAGETPGDDRATGADIVEPYEEAGLTWWIESIDP"
BASE COUNT          347 a          627 c          621 g          370 t
ORIGIN      
        1 cgaacgcgtc ctccaaccga cggactagcc aggatcgatc tccgggctct atggggcgca
       61 cgtggatcat ggcccgcatc gtagccgaag atcggctatg gcgatgtttc tggaagtcta
      121 tcggatccta aaactctcca aacggtctcc gtacgccgtt tggcgcagaa ggggccggga
      181 tcagtgtccc ggccccgaac ggattcttcg atctccgcgc ctaacgatcg gcccgtcttg
      241 cgttggcgtg ggcggcatcg cgtatgtacc gggccaggcc gggagccgtc gcgtcgtagt
      301 tggagcggaa ccgctcgtcg ttgacgtaca tctcggtcag gccgaggtgg atgtcgtggg
      361 tgcactcgta gaaccaccgg ctgatgtgct ggcggtgggc ctcgacggcg tccatcgcct
      421 cctcgctatc cggtgcgaga cccgccgcca gggcagcggc gagacgggcg cgcacctctt
      481 cctcttcggc cttgagctgc tgccagtcct ccttcttgta ggaagcgacg cggcgctgag
      541 actgcctgta ggcctcggtc tcgccccagc gctgcttggc ctcctcggcg tagtcctcgg
      601 gacgaaactc accgaagacc tccagacgct cctccggcgt cagcggtata tccatagtcc
      661 gtgcctccgt ttcgtagtcg atggcttcga ccatcgctct caaacgctcg atcctatcgg
      721 tcagaagcgc acgctggcgg cgcaggtgcc cgtccgcgtc ggtgcgcgga tcgtccacga
      781 tggtcgagat ctctttcagg gtaaacccga gttccctgta gaacaggatc cgctgcagcc
      841 gttcaagatc caacgcctcg tagacgcggt agccagccgc actgcgtccg ccaggcttta
      901 gcaaccctac ctcatcatag tggtgcaacg tccggatcgt cacaccagcg aggtcagcta
      961 cctgacctac cgagtagccc acggtttacc tcctgtctac cgctcagtct aaagccttac
     1021 gttacgtgag ggtcaagtgg ttctcataag attctcgaga acggcaccgg cgatctggcc
     1081 ctggtgaacg ggcgtgcttg gccgtaatct gcatgagact gaggcggtgg ctcgctgcga
     1141 gtaggaggag accttgagat atggactgag cctgcccaac ttcggcgagg ggatcgacgc
     1201 caggacgatc gcgggtctcg cccgcgaagc cgaagaggca ggttgggacg ggttcttcat
     1261 ttgggaccat ctgctcgcgt tctcccctag gcccgtacct gtggttgacc cctggatctc
     1321 cctcgccgcc gcggccatga gcacgagcag aatacggctc gggccgatgg tcacgccgct
     1381 tccccggcgc cgccccgcaa agctggcacg cgagagcgct tcgctggacc acctctccgg
     1441 cggacgcatg atactgggcg tcgggatcgg cgctatgccc tacgagtggg actatctggg
     1501 tgaggaggcg gatatgcggg tccgcggcgc catgctcgat gagggactcg atgtgctaac
     1561 gggactgtgg acaggccagc ccttcgcaca ccgcggcaag cactatagga tccagggaga
     1621 gagtcctgat gaagactggc gggcggtgtt ctatccgccg ccacttcaac gtccacgcgt
     1681 cccgatctgg gtcgccggca catggcccgt caagcctccg ttccgacgcg ccgcgcgctg
     1741 ggacggcgta ttcccgatga aggcagaagg ccggaggata ctcccgatga cccccgagga
     1801 cgtacgccac gtgaaacgct acgtagcgga gcatcgtacc gaagaagggc ctttcgacgt
     1861 cgtcgtcgcc ggagagaccc ccggcgacga ccgggcaact ggtgctgaca tcgtcgaacc
     1921 ttacgaggaa gctggcctca cctggtggat agagagcata gatcc
//
LOCUS       DATAIR010000006         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_369702, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000006 DATAIR010000000
VERSION     DATAIR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369702"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1079)
                     /gene="gnd"
                     /locus_tag="VHM69_00170"
     CDS             complement(162..1079)
                     /gene="gnd"
                     /locus_tag="VHM69_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX2738829.1"
                     /translation="MDIGIWGMGRMGFNMVRRLVDKGDHRVIAGNRSPGKVDEAVEHG
                     AEGAYSVEEFAGKMESPRVLWTMLPAGDVTDDMIHQFTELADPGDIIVDGANSYFRDS
                     VARAREVRSKGFKWLDAGVSGGIWGYDVGYCTMIGGDADAFEHVEPAFKTLAPENGYA
                     FLGDAGAGHFAKMVHNGVEYGMLQAYAEGFEILQKSRYDYDLRALSSLWNQGSVVRSW
                     LLELAESAFEKDGNLDSISGYVEDSGEGRWTVLEAIDESVPANAIAGSLFARFASRQD
                     DSFAMKVIAALRGEFGGHAIKEAATEHEK"
     gene            complement(1162..2256)
                     /gene="tal"
                     /locus_tag="VHM69_00175"
     CDS             complement(1162..2256)
                     /gene="tal"
                     /locus_tag="VHM69_00175"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2738830.1"
                     /translation="MNERLAKMRELGQAPWVDEISREDIKHGGLKQTIDEGIVGVTSN
                     PAIFQKAIAGSDLYDEQLHELAQKEDDPKEIFWEIARQDIQDACDIFMPVHEETGGQD
                     GFVSLEVQPDIAYDTQATVDEAVRLHEMVDRPNLFVKIPATLPGLVAIEEMISRGKSI
                     NVTLIFSLERYREVARAYTRGLQRLVENGGDPSGVHSVASFFVSRIDSEADRQLEEIG
                     ADQYKGKLAIANAKLAYQIYKQVFGGARWSKLAERGANTQRLLWASTSTKNPDYPDTL
                     YVDDLVGPETVNTMPKKTIEAVMDHGNIRPTLEEGVEEAKQLLKRLREAGLDYEDVTD
                     VLEKEGIQKFAEPFNELLEEIQNKSRQLVS"
     gene            2652..2828
                     /locus_tag="VHM69_00180"
     CDS             2652..2828
                     /locus_tag="VHM69_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738831.1"
                     /translation="MRTIARRLRSLADPGSILSVILGVFVGALFGAHYEPRPDPQVHR
                     PDSRGRTYPENRDD"
     gene            complement(2973..>3568)
                     /locus_tag="VHM69_00185"
     CDS             complement(2973..>3568)
                     /locus_tag="VHM69_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2738832.1"
                     /translation="TRVAMGISDVLTSIFGVILYAGMIIGVFAFLRWKNTEQGRKVWG
                     RVSLRIPFKIGDVIQKVALARFARTLGTLTAAGVPILQAIEITASSSGNWVVENALLK
                     TREGIREGIPIYRPLEDEPIFPPMVTRMIAVGEETGDIDGMLSKIAEFYESEVDATVK
                     ALTSIIEPLMIVVVGGIVGGIIIAMYLPMFKIFELVQ"
BASE COUNT          606 a         1141 c         1031 g          790 t
ORIGIN      
        1 ttgggaaagg ccgcggtggc ttcgtaaaca accagcgtca accggtggct cctctcccac
       61 actttgagct gctcgaaatc gcgcataccc aaagtaatcg gctgcatgcc cctttacctg
      121 aagcctgatg cctgatgcct gatgcctgac acctgacagc ttcatttctc gtgctccgtc
      181 gctgcctcct tgatcgcgtg tccaccgaac tcgccgcgca gggcggcgat gaccttcatg
      241 gcgaaggagt cgtcctggcg ggaggcgaag cgggcgaaga gggagcctgc tatggcgttg
      301 gccggcacgg actcgtctat ggcttcgagg accgtccagc gtccctcgcc cgagtcctcg
      361 acgtagccgc tgatgctatc caggttgccg tccttctcga aggcgctctc ggcgagctcc
      421 agtagccagc tacgtacgac gctcccctgg ttccacaggc tcgagagggc cctgaggtcg
      481 tagtcgtagc gggatttctg gaggatctcg aacccctcag cgtaggcctg gagcatcccg
      541 tactcgacgc cgttgtggac catctttgcg aagtggccag cgcctgcgtc cccgaggaat
      601 gcgtagccgt tctcgggcgc gagggtcttg aaggccggct cgacgtgttc gaaggcgtcg
      661 gcgtcgcccc cgatcatggt gcagtagcca acgtcgtagc cccagatgcc tcccgacaca
      721 ccggcatcga gccacttgaa gcccttgctc ctgacctccc tggccctcgc cacagagtcc
      781 ctgaagtagg agttggctcc gtccacgata atgtcgccgg ggtcggccag ctcggtgaac
      841 tgatggatca tgtcgtccgt cacgtccccg gcggggagca tggtccaaag caccctcgga
      901 gactccatct tgcctgcgaa ctcctccacg ctgtaggcgc cctcggctcc gtgctcgacg
      961 gcctcatcca ccttgccagg cgagcggttg cccgcgatta cgcggtggtc gcccttgtcc
     1021 acgagccggc gcaccatgtt gaagcccatc cgccccatac cccagatacc gatgtccatg
     1081 tcgctcctcc cttcggtcgt cgctaggcat caggcttcag gtttcaggtt ttcctgatgc
     1141 ctgaagcctg taacctgaaa cctaactgac tagttgacgg ctcttgtttt ggatctcctc
     1201 cagcaactcg ttgaagggtt cggcgaattt ctggatgccc tccttctcga gtacgtcggt
     1261 cacgtcctcg tagtcgaggc cggcctccct gagccgcttg aggagttgtt tggcctcttc
     1321 gacgccctcc tccagggtag gcctgatgtt gccgtggtcc atgaccgctt ctatggtctt
     1381 tttgggcatg gtgttgaccg tctcgggtcc gacgaggtcg tccacgtaga gggtgtcggg
     1441 gtagtccggg ttcttggtcg aggtcgatgc ccagagcagg cgctgggtgt ttgcgccgcg
     1501 ctcggcgagt ttgctccacc tggcgccgcc gaagacctgc ttgtagatct ggtatgcaag
     1561 cttggcgttg gctatggcga gcttgccctt gtactggtct gccccgatct cttctagttg
     1621 cctgtccgcc tccgagtcta tcctcgaaac gaagaagctc gccacggagt gtacgcccga
     1681 ggggtctccc ccgttctcga ccagtctctg gaggcctctt gtgtaggcgc gggccacttc
     1741 gcggtagcgt tcgagggaga agatcagggt gacgttaatg gacttgccgc gcgagatcat
     1801 ctcctctatc gccactagcc caggcaatgt cgccggtatc ttgacgaaga ggttgggcct
     1861 gtcgaccatc tcgtgcagac ggacggcctc atcgaccgtg gcctgcgtgt cgtaggctat
     1921 gtcgggctgg acttccagcg agacgaaccc gtcctgtccc cctgtctcct cgtggaccgg
     1981 catgaagatg tcgcaggcgt cctggatgtc ctgcctggcg atctcccaga agatctcctt
     2041 agggtcgtcc tctttctggg cgagctcatg gagttgctcg tcgtagaggt cggagccggc
     2101 tatggccttc tggaagatgg ccgggttcga ggtaacgcca acgatccctt cgtctatggt
     2161 ttgcttgaga ccgccgtgct tgatatcctc gcgcgagatc tcatccaccc acggcgcctg
     2221 ccccagctcc cgcatcttcg ccaaacgctc gttcacctgt ctcctcctaa cgaagcccaa
     2281 tacagggacg gtagtgcatc ccgttccttc agtctacacc gcgataccct agagcttccc
     2341 ggcccaggcg ctccttacca tcgaaggtcg tggatcaaac cctacacatc ctgtcctgca
     2401 gctcgaaatc agggcaacta caagtgtgcc cggcttgacc gggagtcgac ggccgcgtga
     2461 ggcaagttgt ctggcgaccg tcaaccgctc cgagaacctg tgctccaggg taaggtgtgt
     2521 aggttctcgc ctatcgttca gtagctatct gcaccccgga agttccccat gttacaatgc
     2581 attgtatgtg ttactcagtt agctcggctt cattttcggc tttgctgggg accaactaga
     2641 gaggagaacg gatgcgtacc atcgcccgtc gcctgaggtc tctggcggat cccggatcga
     2701 tcctgagcgt gatactcggg gtgttcgtcg gcgctctctt cggggcgcac tacgagcccc
     2761 ggccggaccc ccaagtacac cgtcccgact cgcgggggcg gacatacccg gaaaaccggg
     2821 acgattagcc tagcttctgc gctaatctat tcacccgaga gcgtaggagc gtacgcaatt
     2881 cgcaggtagg ccacgcccgg tcgaggaatg cggcttccgc cttaactgtg cggttgctgc
     2941 accgaacgat atacgcgaag cctacaactg acctactgga cgagttcgaa gattttgaac
     3001 atcggcaggt acatcgcgat tatgatgccg ccgacgatgc cgccgaccac cacgatcatc
     3061 aggggttcta tgatcgaggt cagcgccttc accgtggcgt ccacctcaga ctcgtagaac
     3121 tcggctatct tggaaagcat gccgtctatg tcccccgtct cctctcccac ggcgatcatg
     3181 cgcgttacca tgggcgggaa gatcggctcg tcttcgagcg gcctgtatat cgggatgcct
     3241 tccctgatcc cctccctggt cttcaacagg gcgttctcga cgacccagtt gcccgaagaa
     3301 ctggcggtta tctctatcgc ctgcaatatg ggtactcccg cggcggtcag ggtcccgagg
     3361 gtcctcgcga agcgggcgag ggcgaccttc tggattacgt cccctatctt gaagggtatc
     3421 ctgagcgaga cccttcccca gaccttgcgc ccctgctccg tgttcttcca gcggaggaac
     3481 gcgaagaccc caattatcat cccagcgtac agtattaccc cgaagatgct ggtcaggacg
     3541 tcggagatgc ccatcgccac ccgcgtcg
//
LOCUS       DATAIR010000007         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_369733, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000007 DATAIR010000000
VERSION     DATAIR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369733"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VHM69_00190"
     CDS             <1..910
                     /locus_tag="VHM69_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX2738833.1"
                     /translation="GVWRKARVADMPPEPIAPFALRLVRSTDVFSHIDESLRSTSVEW
                     LQTTARSITSRSDSLYEIATDAGTLHAGWVFDSALDLDPVFPSPHEPRAVESGTGIHV
                     KADRPVFDAETATIFDPIDERSFAYLLPLSPFEALLESASFGPVDQEEDQMSLLRYLR
                     ARHPEARFQVTHVEHGAIPLGFAPPRTTGPRHILLGVKRGLIKPSAGYGIVRIARESK
                     YLARLWRENKPIPPSWRSPWQWRVLDKGFLQLAARDPRLPLALLHRVMGAIPLAQSLR
                     FIDEELPVGQLAPLLRSASPVVLGRS"
     gene            1072..1320
                     /locus_tag="VHM69_00195"
     CDS             1072..1320
                     /locus_tag="VHM69_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738834.1"
                     /translation="MNDGVKILLGALGGAIVALLLAGGFSGGGMGYGMMGGMGQMMSG
                     GMMGGSLFGILFPILFWVLIIALLVVLVVWIVNQTQRR"
     gene            1435..1839
                     /locus_tag="VHM69_00200"
     CDS             1435..1839
                     /locus_tag="VHM69_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738835.1"
                     /translation="MKSDPLDVTRAVVLSRATVRKMKQNLVWASVYNVLAIPVAAVVL
                     YPAYGIVLGPEWSALLMSVSSIIVAVNTVLLKGSRATLNHTYDWSKLIVIGQSYPIPH
                     FFVEQTRSSNRFEGGHPNHRNATRVTASACGQ"
     gene            complement(1875..2090)
                     /locus_tag="VHM69_00205"
     CDS             complement(1875..2090)
                     /locus_tag="VHM69_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2738836.1"
                     /translation="MARKRWSELSSGQRRGVVLSGVVQVTLLIAALLDIWRRPEEEIR
                     GSKRLWTAAAFVNFIGPLSYFIFGRRR"
     gene            complement(2309..3079)
                     /locus_tag="VHM69_00210"
     CDS             complement(2309..3079)
                     /locus_tag="VHM69_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738837.1"
                     /translation="MPEIPIEVAYPVSDELRLHLALGACRLKAGPGEGGAWISGTCQD
                     PTGKRAPTVVEEGGRVRITESEPSFERIPAVFGGIPSYELRFGKAQPFALTVETGASE
                     FEMDLGGVPLKTLTVRQGAGKFELDFSAPNPHPMEVLEVSSGAAGMDLENLANANFSE
                     MRLSGGAAGYELDFGGALARDSEVKIETGLSGVEIEVPASTAARIVAETTLGSVDVGD
                     GFTKQEGAFLTESALAGRTPLLTIRAGVRLGSLQLRAT"
     gene            complement(3158..>3423)
                     /locus_tag="VHM69_00215"
     CDS             complement(3158..>3423)
                     /locus_tag="VHM69_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738838.1"
                     /translation="GSYTIPGLVLIVVGVINLAGASTILRRHRWGAPLSALVSLMWVG
                     WFVVQVAVVGLASWQQPFYFGVGLLILLLAAPSLIGQYRMRSA"
BASE COUNT          633 a         1111 c          999 g          680 t
ORIGIN      
        1 tggcgtctgg cggaaggcca gggtggcaga catgccgccc gagcccatcg ccccgttcgc
       61 cttgcggctg gtgcgttcca ctgacgtttt ctcgcacatc gatgagagcc tgagatccac
      121 ttccgtcgag tggttgcaga ccacggccag atcgatcacg agccggtcgg atagtctcta
      181 cgagatcgcc accgacgccg gcaccctgca cgccggctgg gtgttcgaca gcgccctcga
      241 cctcgacccc gtctttcctt cgccgcatga accgcgggcg gtagagagtg gcactggcat
      301 ccatgtaaag gcagaccggc ctgtcttcga cgctgagacc gccactatct tcgatcctat
      361 cgacgaacgt tcattcgcct acctgctacc gttgagcccc ttcgaggcgc tactcgaatc
      421 cgcctcgttc ggtccggtcg accaggagga agaccagatg tccttgttac ggtacctccg
      481 ggcgcgtcac ccggaggctc gatttcaggt tacccacgta gagcacggcg ccataccgct
      541 cgggttcgcg cctcccagga ccacgggtcc tcggcacatc ctgctcggcg tgaagcgagg
      601 cttgatcaaa cccagcgccg gctacgggat cgtccgtatt gccagggaga gcaaatacct
      661 cgcccgcctg tggcgagaga acaagccgat tccgcctagc tggcggagcc cgtggcagtg
      721 gcgcgtcctc gacaagggat tccttcaact cgccgcgcgg gatccccggc tgccgctcgc
      781 gctcttgcac cgtgtcatgg gcgccatacc cctcgcgcaa tcacttcgtt ttatcgacga
      841 agagcttcct gtggggcaac tcgcaccgct cctcagatca gcctctccgg ttgtgctcgg
      901 cagatcgtag cgggcccgtc gaagacgtcg ttgcctcgtc tcccgctgcg tccgagaaac
      961 acgaccttgg tgagatggtc aattctcctc atgagaatga gtcattctga tcaagcgctt
     1021 cccggccccc gcgatagact ggacctgcaa gagcaataag gaggtcaggt catgaacgac
     1081 ggagtgaaga tcctactcgg ggctttaggt ggagctatag tcgccctatt gctcgccggg
     1141 ggcttctctg gtggagggat gggctacggc atgatgggcg gcatgggaca gatgatgagc
     1201 ggtggcatga tgggcggcag cctcttcggc attctgtttc ccattctgtt ctgggtgctg
     1261 atcatagccc tgctcgtcgt gctcgtcgtc tggatcgtca accagacaca gcgccgctag
     1321 ggtaacttcg agcagagctt ttgcggcata gcggcttgcg aggcatggag cgtgccggtc
     1381 ggacataggt atggccaata ggcaccgatg tcgcaataga gacaggtcgt cttgatgaag
     1441 agcgaccctc ttgacgtgac ccgcgccgtc gtcctctcca gggccactgt caggaagatg
     1501 aagcagaacc tggtgtgggc ctcggtgtac aacgtgctgg ctatcccggt ggcggcagtg
     1561 gtgctttacc cagcttacgg catagtgcta gggcccgaat ggtctgctct cttgatgtcg
     1621 gtctcctcga tcatcgtcgc ggtcaacacc gtgctcctca aggggtcgag ggcgaccttg
     1681 aatcacacgt atgattggtc aaagctaata gtaatcgggc agtcctaccc tatccctcac
     1741 ttcttcgtcg aacaaacgcg gtcttctaat cgtttcgagg gaggccatcc aaaccacagg
     1801 aacgctacca gggtgactgc gtctgcctgc ggccagtaga ttccacgctt tctaattccc
     1861 ctgaggattc gtcctcatcg cctcctgccg aagatgaaat acgagagagg gccgatgaag
     1921 ttgacgaagg ccgccgccgt ccacagccgc ttgctgcccc tgatctcctc ctcaggccgc
     1981 cgccatatgt ctagcagagc cgcgatcagc agcgtaacct gcaccacccc tgagagcacg
     2041 acacctcgtc gctgcccgga gctgagctcg ctccaccgtt tcctcgccat cgccacactc
     2101 ccttcaagag gtactcgatc ctcgggcagg gtaaacgtac agtgcgtggg cgcttcctgc
     2161 ctgatcagca tgaaccatat cgattgtcaa agttggccaa tcgcagcccg gccgacctct
     2221 gtgggtcaca gccatcttct tccgtggcgt ccctgccgcg acgtaaggcg acgtgtcgag
     2281 accctctgca ccatagtttc ggttgcgctc acgtagccct tagctggagg gagccaagcc
     2341 tgacaccggc gcggatcgta agtagcgggg tgcgtccggc caacgcgctc tcagtcaaga
     2401 acgctccctc ctgcttcgtg aacccgtccc cgacgtccac gctaccaagt gtcgtctcgg
     2461 ccacgatcct ggccgccgtg gaggcgggga cttcgatctc caccccgctc agtccggtct
     2521 cgattttgac ctcggagtcc cgcgcgagcg caccgccgaa gtcgagctcg tagccggcgg
     2581 cccctccgga gagccgcatc tcagagaagt tggcgttggc gaggttctcc agatccatgc
     2641 ccgctgctcc actggagact tcgagcacct ccataggatg tggattcggg gctgagaagt
     2701 cgagctcgaa cttgccggcg ccctgcctga ccgtcaacgt cttcaagggg acgccaccca
     2761 ggtccatctc gaactcgctc gctcccgtct cgacggtcag cgcgaaaggt tgcgccttgc
     2821 cgaagcggag ttcgtagctc gggatgccgc cgaagacggc agggatgcgc tcgaaggagg
     2881 gctcggactc ggtgatcctg acccttcccc cttcctcgac cacggtaggc gcccgtttgc
     2941 cggtcggatc ctggcacgtg ccgctgatcc aggctcctcc ctcgccgggt cctgctttca
     3001 agcgacaggc cccgagcgcg agatgcaggc gcagctcgtc cgagacgggg taggcgactt
     3061 ctatcggtat ctctggcatg aaagcctcct ttcgcgatcg actatccttc gagtgatgct
     3121 ggttgaccgc cgtgggaacg ggtgctcgaa gaaacgtcta ggcacttctc atccgatact
     3181 gccctattag cgacggtgcc gcaagtagaa gtatcagcag gccgaccccg aagtagaaag
     3241 gctgctgcca gcttgcgagc ccgacgacgg cgacctgcac cacgaaccac cccacccaca
     3301 tcaaactcac cagagccgac aaaggagcgc cccaccggtg gcgacgcaat attgtagacg
     3361 cgcccgcgag gttgatgaca cccacaacga tcagcaccag ccctgggatt gtgtagctac
     3421 cga
//
LOCUS       DATAIR010000008         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_277483, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000008 DATAIR010000000
VERSION     DATAIR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277483"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHM69_00220"
     CDS             <1..420
                     /locus_tag="VHM69_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX2738839.1"
                     /translation="PTESVLRAISSADAIVFCPSNPVVSIGPILAVPGMREAIASSSA
                     PKVAVSPIVGGRALKGPADRMLASLGHEVSATGVARMYAGLVDGMVVDRIDEGEQPGI
                     EAAGMRTLVTESVMRDAGDRARLASETLEFGAGMVAR"
     gene            417..1025
                     /gene="cofC"
                     /locus_tag="VHM69_00225"
     CDS             417..1025
                     /gene="cofC"
                     /locus_tag="VHM69_00225"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX2738840.1"
                     /translation="MSISISAVVPVKDLRGTKSRLAPVLNPGARAGLTLYMMGRVVAA
                     LREAGVEDVCVVSPDIIVLNEAHRRGATPLIQESRGLNPALEEGRRRALGLGASTLLV
                     LPADLPLLDAEDVRAVLEEAGEGKSVVISPDGARSGTNALLIQPPDALPFAFGPDSFE
                     AHLEAARSRKLEIRICERCHLAFDLDTAEDLARLNEKRAPSP"
     gene            1022..1765
                     /gene="cofE"
                     /locus_tag="VHM69_00230"
     CDS             1022..1765
                     /gene="cofE"
                     /locus_tag="VHM69_00230"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2738841.1"
                     /translation="MRGLEVLGVEGFPEVRPGDDLERMISGTVAHDLRPGDVLVVTHK
                     IVSKAEGCLVDLRTVEPSTLAKGYATRYGKDPRQIEVVLRESRRIVRMDRGIVISETH
                     HGFVCANAGVDASNVPGDDTVCLLPVDPDASAARLRDALVEDPGVNVAVVISDSFGRA
                     WRHGITDVAIGVAGMDPIADYRGQRDPHGYPMEASVLAVADELAAAAELVMGKTEGIP
                     LAIVRGYPYSPASGSARDLLMPPERDMFR"
     gene            2007..>2188
                     /locus_tag="VHM69_00235"
     CDS             2007..>2188
                     /locus_tag="VHM69_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(40)"
                     /protein_id="HEX2738842.1"
                     /translation="MNNLRAYPVYLGIRGALALFFTLWATVAMVYRIEVVDLDPLRLV
                     LLGTALEVSVFLFEVPT"
BASE COUNT          363 a          697 c          733 g          395 t
ORIGIN      
        1 cctaccgaga gtgtcttgag agctatctcc agtgccgacg ccatcgtttt ctgtccttct
       61 aatcccgtcg tgagcatcgg cccaattctg gctgtgcctg gcatgcgaga ggccattgcg
      121 tcgtcttccg ccccgaaggt ggccgtcagc ccgatcgttg gcgggcgagc tttgaaaggt
      181 cctgctgaca ggatgctcgc ctcgctaggc cacgaggtct cggccacggg cgtggccagg
      241 atgtacgcag gtctggttga cggtatggtc gtcgaccgca tagacgaggg cgagcagccc
      301 ggcatcgagg ccgccgggat gcgcactctc gtaacggaat cggtgatgcg cgacgccgga
      361 gaccgggcgc gcctcgcgtc ggagacgctg gagttcggcg cagggatggt ggcgcgttga
      421 gcatctctat ctccgcggtc gtacctgtga aggacctgcg tgggaccaag agccgcctcg
      481 cccctgtcct caaccccggc gccagggcag ggctcaccct ttacatgatg ggccgcgtcg
      541 tggccgcgct cagggaggcg ggcgtcgagg acgtctgcgt ggtgagccct gacattatcg
      601 tcctgaacga ggcacacagg cgcggcgcca cacccctcat ccaggagagc cgggggctca
      661 accccgccct cgaagaagga cgccgccggg cgctcggcct cggggcctcg acgctgctcg
      721 tgctccctgc cgacctgccg ttactggatg ctgaggacgt gcgggccgtg ctggaggagg
      781 caggcgaggg aaaatctgtg gtcatctcgc ctgacggagc gcgttcgggt acgaacgcgc
      841 tcctgatcca acctcctgac gccctgccct tcgccttcgg tccggacagc ttcgaggcgc
      901 acctcgaggc ggcccgcagt cgcaaactcg agatcaggat ctgcgagcgg tgccatctcg
      961 ctttcgacct ggacacagcg gaggacctgg cgcgcctgaa cgagaagcga gcgccgagcc
     1021 cgtgagaggg ttggaggtgc tgggagtcga gggtttcccc gaagtccgtc ccggcgacga
     1081 cctggagcgt atgatctccg gcactgtcgc ccacgacctg cgccccggtg acgtcctcgt
     1141 ggtgacccac aagatcgtca gcaaggccga gggttgtctg gtcgacctcc gcacggtcga
     1201 gccctccacg ctggcgaagg gttacgcgac gcgctacggg aaggacccgc gccagatcga
     1261 ggtcgttttg agggagagcc gacgcatcgt acgcatggac cgtggcatcg tcatctcgga
     1321 gacccaccac ggcttcgtct gcgccaacgc cggcgtagac gcctcgaacg tacccggcga
     1381 cgacaccgtc tgcctccttc ccgttgaccc agacgcctcc gcggcgagat tgcgcgacgc
     1441 attagttgag gacccaggcg tgaatgtggc agtggtgatc tcggactcgt tcgggcgggc
     1501 ctggcggcac ggcataacgg acgtcgccat cggggtcgcc ggtatggacc ccatcgcaga
     1561 ctacagggga cagagggacc cgcacggcta cccgatggag gcgagcgtcc tcgcagtcgc
     1621 cgacgagctc gccgccgccg ccgaactggt gatgggaaag accgaaggga tccctctggc
     1681 tatcgtgcgg ggctatcctt attcgcctgc ttcaggcagc gccagggacc tcctcatgcc
     1741 gcccgagagg gatatgttca ggtaggccgc tcgcgttctt gacgccggcc tggaggttgg
     1801 ggtacgatcg acgtcatgac aattcctcgc tagacggcgg aagctgaaca ggagaaccac
     1861 gcggttctcg ctcgctccac cgtacctccg taacgaccga tccggaactc tggcatgtgt
     1921 ctcgcgacac tggcgtcatg gatcggggcg ttacatcagt ggcatcacgg gattgaagcg
     1981 gcgcatttcg agaaagctgg acttgcatga acaacttgag agcatatccg gtctacctcg
     2041 gtatcagagg ggctttggcg ctcttcttca cgctgtgggc caccgtcgcg atggtgtacc
     2101 ggatcgaggt cgtggacctg gacccgctgc ggctggtgct ccttggtacg gcgctggagg
     2161 tctccgtgtt cctcttcgag gttcccac
//
LOCUS       DATAIR010000009         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_415969, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000009 DATAIR010000000
VERSION     DATAIR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415969"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /gene="map"
                     /locus_tag="VHM69_00240"
     CDS             complement(<1..695)
                     /gene="map"
                     /locus_tag="VHM69_00240"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2738843.1"
                     /translation="MIVRKSKTELEAMREGGRITAACLQMLRENVRPGVTTRELDRLA
                     EEFIHERGGKPEFKGYQGFPASICASPNAMIVHGIPGSYRLKNGDIISLDVGVRYEGF
                     VTDSATTVPVGDVPEEVDGLLGTTRECLAAATEQMRAGNRLGDVGHAIQSLAESRGYG
                     VVRDLVSHGVGRQMHEDPQIPNYGSPGTGPRLLPGMTFAIEPMITLGDYGIRLSEWDG
                     WSIYTADGSLSAH"
     gene            complement(692..1315)
                     /locus_tag="VHM69_00245"
     CDS             complement(692..1315)
                     /locus_tag="VHM69_00245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2738844.1"
                     /translation="MGPQGAGKGTQAHRIAERVGAKHISTGDIVRAEIKSGSELGQKV
                     QDYNDRGELVPDEIIVEMAKPYLDDADAWILDGFPRNEAQAKALDKALEELGEDLDAV
                     VALEAPDDALVERLSGRRQSEATGNIYHVEYDPPPEDGDDPGPFIQRKDDAEDAIRRR
                     LEIYHEQTEPLKDYYAERGLLVTIDAEQDIPKVTEDILEAVSEKDDA"
     gene            complement(1327..>2317)
                     /gene="secY"
                     /locus_tag="VHM69_00250"
     CDS             complement(1327..>2317)
                     /gene="secY"
                     /locus_tag="VHM69_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2738845.1"
                     /translation="ELAKEGETGQQKITQYTRYFTLALALIQSVAMVFFFRSAQGGGA
                     LAGTSPIEILVAVVTLTAGVMLTMWLGELISQRGLGNGISLIITASILSQAPRAIQSL
                     IENGDVLTMVILALLAVMIVAAIVFVNEGQRRIPITYAKRQVGRRMSQGGTTYLPLKV
                     NMAGVIPIIFASSLLLFPVVLAQLAAGGDPDSILGRLANFFDPSNAPYLILYALLIIM
                     FTYFYTAVQFNPVEHADNLKKSGGYVPGYRPGQPTAIYLNNVLTRITLFGALFLAVVA
                     VLPYLIAGGLNLGNSIFLGGTSMLIVVGVSLDTVRQLESQLMMRNYEGFLKKR"
BASE COUNT          403 a          712 c          751 g          451 t
ORIGIN      
        1 aagtgggcgg agagcgagcc gtccgccgtg tagatggacc acccgtccca ctcgctcagg
       61 cggatgccgt agtcgccgag tgtgatcatg ggctctatag cgaaggtcat gccaggaagt
      121 aaccgcggac ccgtacccgg actgccgtag tttgggatct gggggtcctc gtgcatctgg
      181 cgcccgaccc cgtgggagac gaggtccctg acaaccccgt aaccgcgcga ctcggcgagt
      241 gactgtatcg cgtgtcccac gtcgccgagc ctgttgccag cccgcatctg ctccgtcgcg
      301 gccgcaaggc actcgcgggt agtaccgagc agcccgtcga cctcctcggg cacatcgccg
      361 acgggaaccg tcgtcgcgga gtccgtgacg aagccctcat aacggactcc gacgtcgagg
      421 gagatgatat cgccgttctt caggcggtaa gagcctggga tgccgtgtac gatcatggcg
      481 ttcggtgagg cgcagatcga ggcaggaaac ccctggtacc ccttgaactc tggcttgccg
      541 ccacgttcgt ggatgaactc ctcggcgagg cggtcgagct cgcgtgtcgt cacgccgggg
      601 cggacgttct ccctgagcat ctgcaggcag gccgcggtta tgcgcccgcc ctcgcgcatc
      661 gcttcgagct cggtcttgct cttgcgtacg atcaagcgtc gtccttttcg ctgaccgctt
      721 cgaggatgtc ctccgtgacc ttggggatgt cctgctcggc gtcgatagtc acgaggaggc
      781 cccgctcggc gtagtagtcc ttcagtggtt ccgtctgctc gtggtagatc tcgagcctgc
      841 gcctgatggc atcctcggcg tcgtccttgc gctggataaa cggaccgggg tcgtccccgt
      901 cctcaggtgg cgggtcgtat tcgacatgat aaatgttccc cgtggcctca ctctgacggc
      961 gcccggagag ccgctcaacg agcgcatcgt ccggagcttc gagggcgacg acagcgtcga
     1021 ggtcttcccc gagctcctca agggccttgt cgagcgcctt cgcctgggct tcgttgcggg
     1081 ggaagccgtc gaggatccag gcgtcagcgt cgtcgaggta gggtttcgcc atctcgacga
     1141 tgatctcgtc cgggacgagc tcgcccctgt cgttgtagtc ctgaaccttc tggccgagct
     1201 cggagcctga ctttatctcg gctcgcacga tgtctcccgt ggagatgtgc ttcgcgccca
     1261 ccctttcggc gatgcgatgc gcctgcgtcc ccttgcctgc cccctggggg cccaatagaa
     1321 taatcctcat cgtttcttca agaacccttc gtagttgcgc atcattaact ggctctccaa
     1381 ctggcgtacc gtgtcaagcg agacgccgac cacgatcagc atcgaggtgc cgccgaggaa
     1441 gatcgagttg ccaaggttca ggccaccagc gatcaggtaa ggcagcacag cgacgactgc
     1501 caggaagagc gcgccgaaga gcgtgatcct ggtcagcacg ttgttgaggt agatggccgt
     1561 cggctggccg ggcctgtagc ctgggacgta gccgccgctc ttcttcaggt tgtctgcgtg
     1621 ctcgacgggg ttgaactgca ccgccgtata gaagtaggtg aacatgatga taaggagcgc
     1681 gtagaggatc aggtacggcg cgttgctcgg gtcgaagaag ttcgccagac gaccgaggat
     1741 cgagtcaggg tctcctcccg cggcaagctg ggcgagcacg acggggaaga gcagaagtga
     1801 agaagcaaag atgataggta tgaccccggc catgttcacc ttgaggggca gatatgtcgt
     1861 cccgccctgg ctcatcctac gcccgacctg acgctttgcg taggtgatcg ggatcctgcg
     1921 ctggccctcg ttgacgaaca cgatggcggc gacgatcatg accgcgagca gcgcgaggat
     1981 caccatggtc aggacatccc cgttctcaat gagagactga atggcgcgcg gcgcctggga
     2041 gaggatcgaa gccgtaatga tgagcgagat gccgttgccg aggccgcgct ggcttatgag
     2101 ctcgccgagc cacatcgtga gcatcacgcc ggccgtaaga gttactacgg cgacgagtat
     2161 ctcgatcggg ctcgtccccg cgagcgcccc gccgccctgt gccgagcgga aaaagaagac
     2221 catcgcgacg gactggatga gcgcgagcgc gagcgtgaag tagcgggtgt actgtgtgat
     2281 cttctgctgc cccgtctcgc cctctttggc cagttcc
//
LOCUS       DATAIR010000010         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_46428, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000010 DATAIR010000000
VERSION     DATAIR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46428"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..636)
                     /locus_tag="VHM69_00255"
     CDS             complement(226..636)
                     /locus_tag="VHM69_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738846.1"
                     /translation="MRFAAAWVAALRQDELARKREETRKRKAEEEAAAWRLFVKTEQR
                     QLQLRKDGQLARLLGQPLPGELPAILQRLASEDQRQAERGLVALMSGGKTYYKAIDDL
                     APDDMPARIAANRLRTTWLKERGDEWLGNGESQP"
     gene            979..1404
                     /locus_tag="VHM69_00260"
     CDS             979..1404
                     /locus_tag="VHM69_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738847.1"
                     /translation="MVNWTQSLVLGFFAVVWISLVAILLVEPGIFDDAMRLPEGNHRL
                     ADLAFLGAISAFIALLVVGVLRRWRWTFWLVLVAFLIGGVLRVPASVLALAGMIPESG
                     PTWYVLYQAVLGVAQVVIGVLMLADYKRAGTWGLSETPP"
     gene            1460..1876
                     /locus_tag="VHM69_00265"
     CDS             1460..1876
                     /locus_tag="VHM69_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2738848.1"
                     /translation="MTDEEYRSFLLGRARTATLATVRDDGRPHAAPIWFDLDGDTLVF
                     TTGEGSIKALNMRRDPRVSLCIDEDRPPFHFVLIEGTAELEADDPDLLYWATRLGGRY
                     MGVERAEEYGRRNAVEGELLVRVTPRKIVAHKNISD"
     gene            1942..2640
                     /locus_tag="VHM69_00270"
     CDS             1942..2640
                     /locus_tag="VHM69_00270"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2738849.1"
                     /translation="MMKEQDGKGETGEKQGLNRLADRLREAVSHPLGEWRDVYEGFSP
                     VDLETGERRPRIPPPDADSRRAAVLIPILIEGEDARVVYTVRKDHLADHAGQISFPGG
                     SIESGDGSLLETALREAEEEIDLSPDLVEVIGELEEMYIPPSNFRVSPYVGLLPPEAE
                     MVLAPDEVEEIFTVSLATLTAPETFRKMPWRYEGRDYEVPVFAVEGSRNIWGATAAMT
                     AALLARLGWREYRR"
     gene            2650..3051
                     /locus_tag="VHM69_00275"
     CDS             2650..3051
                     /locus_tag="VHM69_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738850.1"
                     /translation="MFRFVAIALFSLMFLVQAGDAYGHFLTLADPAPEAARFGITVGA
                     EVFRTAILLILALSVALGALLAVVGLRAGRASLFHHGALACSLGYMVYGLYQVADGAL
                     QLGSAVVVVAGLIYVVLGGLAYAMHRTVLEI"
     gene            complement(3148..>3792)
                     /locus_tag="VHM69_00280"
     CDS             complement(3148..>3792)
                     /locus_tag="VHM69_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2738851.1"
                     /translation="VYHYRELAEPPPRPGYRRRSSVLDPYLPYLVARWNEGCRSVKRL
                     HQEIREMGYRHSVDTVNRLLSSFRHTEERGGKPFHAPRAKRGSIAGASPTAKNVAALF
                     MRREEMLNGEQKEYLERLCASDQALADARRLTQDFAKMVRGLGGEELDGWLEEAAGCE
                     ASVMKKFAAGLKKDLPAVRTGLTETWSNGPVEGFVNKLKLVKRQGYGRARVLAA"
BASE COUNT          600 a         1193 c         1223 g          776 t
ORIGIN      
        1 gaacgccggc gacgtcgtcg ataccctgaa gatgatcggt gacaagagcg aggcgctctt
       61 cctgctcgac agggaactgg gccagcgccg gacggaggaa caaccttcag actgacatga
      121 gttgaggcgg cgccttggcg accagtggtc agcccccagt aagagtgaaa tagcgacagg
      181 ctgacggcgt tcgcgtagcg ggcctagacc actgcgtatt tcgctttaag gttgactctc
      241 cccgttgcca agccattcgt caccacgctc tttgagccat gttgttctca gcctgtttgc
      301 ggcgatcctg gcaggcatat catcaggagc gaggtcgtct atggctttgt agtacgtctt
      361 gccgccgctc atcagcgcca ccagcccgcg ttcggcctgc ctttggtctt cggaggccag
      421 cctttgcagg atcgcaggta gctctccagg caacggttgg ccgagcaacc tggccagctg
      481 gccgtccttg cgtagctgga gttgacgctg ctcggtctta acgaagagtc tccaggcggc
      541 tgcttcttcc tctgcctttc tcttccttgt ctcctcgcgc ttgcgggcga gctcatcctg
      601 cctgagggcg gcgacccagg ccgccgcgaa tctcacccgg cttctctccc ttgcccgctc
      661 tcgttcacga cctcgcgcga gatactcttc ccatttccgt gtctgagcgt ccttcgacac
      721 cagaagcatc cttctttcgt attggcgccc gggccccgaa agaggagcgc gcccgggctc
      781 ttgtacgtgc agggaaggcg gctgcggctc catcaagagg tatccggctt tcctattaat
      841 gcttattgta tcatatcgtg acttcctcat gggcggtccc gccgccgggg tgatgcggcc
      901 cgacgacgat gtttctgtgg gtgccctggc gttgttatca tcagtgtggg gtatctgacc
      961 gttcgtgata ggatcgctgt ggtcaactgg acacaatctc tcgtgctcgg gttcttcgct
     1021 gtggtttgga tcagcctcgt ggcaatcctc ctggtggagc ctgggatctt cgacgatgcc
     1081 atgaggcttc cagaaggaaa ccaccgactg gctgacctgg cgttcctggg agcgatctct
     1141 gccttcatcg cactgctagt ggtcggggtg ctgaggcgct ggcgctggac gttctggctg
     1201 gtcctggtcg cgtttctgat cggcggcgtg ctgcgcgtac cggcctcggt cctggcactg
     1261 gcagggatga tcccggaatc aggaccgacc tggtacgtgc tgtaccaggc cgtactaggc
     1321 gttgcgcagg tcgtcatcgg agtgctgatg ctggcggact acaagcgagc cggtacgtgg
     1381 gggctctctg aaacccctcc gtagtagtat gtagcgtcgg aattcacgat agtgggggcg
     1441 caacgaagtg tcgcaacgga tgacggacga ggagtacagg agcttcctgc tcggccgcgc
     1501 acggaccgcc acgctcgcca ccgtacgtga cgacggccgg ccccacgctg ccccgatctg
     1561 gttcgacctc gacggggata cgctcgtgtt cacgaccggg gagggttcga tcaaggctct
     1621 caacatgcgc cgcgatccac gcgtgagcct gtgcatcgac gaagaccggc caccgttcca
     1681 tttcgtcctg atcgaaggca cggcggaact tgaggctgac gatcccgacc tcctttactg
     1741 ggcgacgcgg ctcggaggac gctacatggg cgtcgaaagg gccgaggagt acggcaggcg
     1801 caacgcggtt gagggtgaac tgctggtgcg ggtaactccg cggaagatcg tcgcgcataa
     1861 gaacatatcc gactgaatac acggtcgcgg atagcctcgt cgagtgcgta ccgttgcacg
     1921 gacggtgcgc tatgatctcg aatgatgaaa gaacaagacg gcaagggcga gacaggggag
     1981 aagcagggtc tgaacaggct cgcggatcgt ctgcgtgagg cggtctcgca tccgctgggc
     2041 gagtggcgcg acgtctacga gggcttctcg ccggtggatc tggaaaccgg agagaggcgt
     2101 ccgcggattc cgcctccgga cgccgattcg cggcgcgcgg cggtgctcat accgatcctc
     2161 atagaaggcg aggacgcgcg ggtcgtctat acggtgagga aggaccacct cgccgaccac
     2221 gcgggccaga tctccttccc cggaggcagt atcgagtcgg gcgacggctc gctcctcgag
     2281 accgcactgc gcgaagccga ggaggagatc gacctgagcc cggacctggt cgaggtgatc
     2341 ggggagctcg aggagatgta cataccgccc tcgaacttcc gcgtcagccc gtacgtgggg
     2401 ctgctgccgc ccgaggccga gatggtgctc gcacccgacg aggtggagga gatcttcacg
     2461 gtctcgctgg ctaccctcac cgccccggag acgttccgca agatgccgtg gcgttacgag
     2521 ggccgcgact acgaggtccc ggtcttcgcc gtcgagggtt cgcgcaacat ctggggcgcg
     2581 acggccgcga tgaccgcggc cctgctggcg cgcctgggct ggcgagagta ccggcgctga
     2641 ggggacgttg tgtttcggtt tgtcgcgata gcgctcttca gcctcatgtt cctggtacag
     2701 gcaggggacg cctacggaca ctttctgacc ctcgccgacc ctgcgcccga ggcggcgcgg
     2761 ttcggcatta ccgtcggggc cgaggtcttt cgcacggcca tactcctgat ccttgccctg
     2821 tccgtggccc tcggcgcgct tcttgccgtg gtgggattgc gcgccgggag agcctcgctc
     2881 tttcaccatg gcgccctggc gtgctcactc ggatatatgg tgtacggact ctatcaggtg
     2941 gctgatggtg ctctgcaact cgggtcggcc gtcgttgtcg ttgccggctt gatctacgtg
     3001 gttctcggcg gcctcgccta cgcgatgcat cggacggtat tggagatttg aagttccggc
     3061 gtgcagactg actcatgaag cctgggctct cccgcaaact tggtgaagcg gtgcgtcctc
     3121 cgtcgaaccg ctccgacggt cggcggttca ggcggccagg accctcgccc tgccgtagcc
     3181 ctggcgcttc acgagcttga gcttgttcac gaacccttcc accggaccgt tggaccaggt
     3241 ctccgtcaac ccggtgcgca ccgccgggag gtccttcttc aaaccggccg cgaacttctt
     3301 catcaccgac gcttcgcacc cggcggcctc ctccagccac ccgtcgagct cctcgccccc
     3361 tagccctcgg accatcttgg cgaaatcctg cgtgagacgg cgggcgtcgg caagcgcttg
     3421 gtcggaggcg cagagtcttt ccaggtactc cttttgctcc ccgttcaaca tctcctccct
     3481 gcgcatgaac agggccgcga cgttcttggc ggtcggggac gcaccggcta tggaacccct
     3541 cttcgccctc ggggcgtgga acggcttccc tcctcgctcc tcggtgtgcc tgaagctgga
     3601 gagcagccgg ttcacggtgt cgacgctgtg gcggtagccc atctcccgaa tctcctggtg
     3661 tagccgcttg acgctccggc agccctcgtt ccaccttgcg accaggtagg gcaggtaagg
     3721 gtccagcacg ctcgacctcc tcctgtaccc ggggcgcggc ggaggttccg cgagctccct
     3781 gtagtggtag ac
//
LOCUS       DATAIR010000011         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_231032, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000011 DATAIR010000000
VERSION     DATAIR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231032"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VHM69_00285"
     CDS             <1..466
                     /locus_tag="VHM69_00285"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2738852.1"
                     /translation="AEETETDLFGEQAVLCGGLTALLKAGFDTLVEAGYQPELAYYEC
                     VNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTAGPKIIDEAAKERMRGILADIQSGR
                     WAKDWVLENKAGGSSFLAMRRREAESQVEKVGQELRALAAEGIETPAGGDNR"
     gene            463..1419
                     /locus_tag="VHM69_00290"
     CDS             463..1419
                     /locus_tag="VHM69_00290"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX2738853.1"
                     /translation="MSRTAFAASDAEWAYHGGDLIRMGDIRLSPATHALNYGTGVFEG
                     IRAYWSEERGTLQVLKMREHYERFEKSCRLLRIDFPTTVDELCDITLEILRRNAPRED
                     TYIRPLAFKAAESVGVNLKGESELSIFTVPMGNYVELTGLKVCVSSWRRTPDNAIPAR
                     AKCTGSYVNTALAVDQAHMAGYDDAIFLTQDGQVSEASAANIFLVRKGQLITPPVTAD
                     ILEGITRDAVMELAGKELEMPVIERDVDRTELYAADEVFLSGTGFQIAPVVEVDDRPI
                     GTGSIGPVAERLQELYFKAARGEWDEYADWTVAVEVAEGARQ"
BASE COUNT          316 a          451 c          512 g          256 t
ORIGIN      
        1 cgcggaagaa acagagacag acctcttcgg cgagcaggca gttttgtgtg gaggactgac
       61 ggcgctcttg aaggccgggt tcgacacgct cgtggaggca ggctatcagc ccgagctcgc
      121 gtactacgag tgcgtaaacg aattgaagtt gatcgtcgac ctcatctacg agggcgggct
      181 ggcggggatg cggtactcga tctcgaacac cgcagagtac ggcgactaca cggcgggacc
      241 gaagatcatt gacgaagctg ccaaagagag gatgcgcggt atcctcgctg acatccagag
      301 cggcaggtgg gccaaagact gggtgctcga aaacaaggcc ggggggtcga gcttcctcgc
      361 gatgcgtcgc agggaggccg agtcgcaggt cgagaaggtc ggacaggagt tgcgggcgct
      421 cgcggccgag ggcatcgaga cacccgctgg aggtgataac agatgagtcg tacagcattc
      481 gctgcgtcgg acgcagagtg ggcctaccac ggtggagacc tgatcaggat gggcgacata
      541 cgcctctccc cggcgaccca cgccctgaac tacggcactg gcgtcttcga gggcatccgt
      601 gcttactgga gcgaggagcg cgggacgcta caggtcctaa agatgcgcga gcactacgag
      661 cgcttcgaga agtcctgccg gctgctacgc atagatttcc caaccaccgt tgacgaactg
      721 tgcgacatca cgctcgagat cctgcggcgc aacgccccgc gcgaggacac atacatcagg
      781 cccctggcgt tcaaggcggc cgagtccgtc ggggtgaacc tgaaagggga gtcggagctc
      841 tccatcttca cggtccctat gggcaactac gtcgagctca cgggcctgaa ggtctgtgtc
      901 tcatcctggc ggcgcacgcc tgacaacgca atccctgccc gcgccaagtg cacgggctcc
      961 tacgttaata cggcgctggc cgtggatcag gcacacatgg caggctacga cgacgccatc
     1021 ttcctcaccc aggacgggca ggtctcggag gcgagcgcgg ccaacatctt cctcgtcagg
     1081 aagggccagc tcatcacgcc gccggttacg gcggacatcc tggaaggaat tacacgcgac
     1141 gccgtcatgg agctcgctgg caaggagctg gagatgcccg tcatcgagcg cgacgtcgac
     1201 aggacggagc tgtacgcggc agacgaggtc ttcctttccg gtacggggtt ccagatcgca
     1261 cccgttgtag aggtggacga ccgtcctatc gggacgggta gcatcggacc cgtcgccgag
     1321 cggttgcagg agctgtactt caaggccgcg cgcggcgagt gggatgagta cgccgactgg
     1381 acggtggccg tcgaggttgc ggaaggagcg aggcagtgag atactgccac ccttcatgaa
     1441 ggtgagctat taacaaagtg cgttcctctc tcatcatcct acgactaacc agggtggccc
     1501 cagggcgaat tatccgtccc ggtctctagc cgggc
//
LOCUS       DATAIR010000012         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416060, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000012 DATAIR010000000
VERSION     DATAIR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416060"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..941)
                     /locus_tag="VHM69_00295"
     CDS             complement(174..941)
                     /locus_tag="VHM69_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX2738854.1"
                     /translation="MQITPDHEIRALVGRLTDEIRRSLNGSLVGLYVYGSLVTGDFEK
                     ESSDIDLLAVVSSDIEGETFERLDRMHARFVEDHPAWEDRIEVAYVSARALWKFKTET
                     NNIAVVSPGEPFHLKAAGRDWLMNWHTVREMGVTLYGPPPQTLIPEISRAEFVESARE
                     HARSWEEWVHEMRTPGSQAYAVLTMCRALYTRTHGEQVSKKKAALWAKAQLPQWAPLI
                     ERSWSQRSDVRDEETDEEEFYETVRFVHDVACRVETQ"
     gene            1044..1412
                     /locus_tag="VHM69_00300"
     CDS             1044..1412
                     /locus_tag="VHM69_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX2738855.1"
                     /translation="MNKGTLDFYELVERALEGLPPELSALLDNVAIVVEDWPDYSTPL
                     VADGPGDTLYGLYEGVPLTERGAGYYGFLPDKITIFRGPLERDFQTDELEEQVRITVV
                     HEIAHYFGFDEERLEELGWG"
     gene            1409..1852
                     /locus_tag="VHM69_00305"
     CDS             1409..1852
                     /locus_tag="VHM69_00305"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2738856.1"
                     /translation="MNSGAAGSVRIEQRIEAPLERVAAYISDFRNAREWMVGVEAVEP
                     LGEESFRLTLESPVGRIEPAVRILERDSQSISWVYTSAIEGGGRVDISPDTDGGCLVA
                     YTGEFHLKRKFLDRAARLVGVERFARSNGERSLKRLKHLMEARRY"
     gene            1852..2037
                     /locus_tag="VHM69_00310"
     CDS             1852..2037
                     /locus_tag="VHM69_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2738857.1"
                     /translation="MSVGLIEILLVFMVFLVLGPRRIANFFRSMGTGIHDFMETLGRD
                     KDKEQLPGEDDEHKRNS"
     gene            complement(2041..2967)
                     /locus_tag="VHM69_00315"
     CDS             complement(2041..2967)
                     /locus_tag="VHM69_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738858.1"
                     /translation="MEGQDGHNGRQPSDERPDGADTTEVEGGRGEISEPEELRARLVI
                     KDNHIRELYEEITASRLAADEARATKEAGAGHIESLESERARLKERIRNLEEEVRERR
                     RRRESFERQVARLERELERKNGEISHRDYLLERRAEQMEAANQRTEELASRKDLALQD
                     ALRRVEGLERDLEEREGEISNLSTTIGKLREELEAEREQRVHLADPANRLRAGIDLFN
                     ESEQRHSMNALSRTLGQPEVYVELDDGDEPPALLTFTWQGVTWQTYAADPGLAVEEPR
                     VYLRSAGEDLSGVDRNPPNARVGPGDRVVLGL"
     gene            complement(2974..3912)
                     /locus_tag="VHM69_00320"
     CDS             complement(2974..3912)
                     /locus_tag="VHM69_00320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2738859.1"
                     /translation="MFTRTRTEPVPEVGADAPEFNLPSAQGGQLRLSMRTARGPVVVA
                     FYRPGDEEDVEYFKALAAKEQEINMAAGSVVAIGVAEPAQARNFARASGLKSYVLYDY
                     ARVASRQWGLLDKDRKRGDSSRPAVFVVGPDRKVAHAWVGERPAPEEILAKISEISGL
                     PKPAEKPEEPKKEEAGGEKPKKMSAEEREKIKAERRAAREAGKSVKSGQAGESTSESE
                     EAPKKLSAEDRERIKAERRAAREAGKSVRTGASATTEAPAETSGSEDKPKKMSAEERE
                     RIKAERRAAREAGKSLKQPASQPDPDSEEEASKKEG"
BASE COUNT          705 a         1476 c         1309 g          916 t
ORIGIN      
        1 caacgtatac ctcccataaa atatcaagtg gagtagtcat tctagcctgc cggatcgcta
       61 cccggcgcaa cgttgtgaac gacgcaaacc cggtcctgga atctgggtga ggagcgcgaa
      121 ggaccggctc tgtcctggag ctaacgcagg tggctctttg tggcgcgtcg aggtcattgc
      181 gtttcgaccc tacaggcaac gtcgtgaacg aagcgcaccg tctcgtagaa ctcttcctca
      241 tccgtttcct cgtctcgcac gtcgctcctc tggctccagc tccgctcgat caacggagcc
      301 cactgcggca actgcgcctt cgcccacagc gccgccttct tcttggacac ctgctcgcca
      361 tgcgtacgcg tatacagcgc tctgcacata gtaagcacgg cataagcctg cgatcccggg
      421 gtgcgcatct cgtggaccca ttcttcccag ctcctggcgt gttctcgtgc cgactcgacg
      481 aactcggccc tcgagatctc agggatcagg gtttggggcg gcggaccgta gagagtgacg
      541 cccatttcgc gaactgtgtg ccagttcata agccaatctc ttccggccgc cttcaggtgg
      601 aacggttcgc cggggctgac gaccgcaatg ttgttcgtct ccgtcttgaa cttccacagc
      661 gcacgggcgg acacgtaagc cacttcgata cgatcttccc acgcagggtg gtcctcgacg
      721 aagcgcgcgt gcattctatc caaccgctcg aaggtttcgc cctcgatatc tgagctgaca
      781 accgccagca agtcgatgtc gctgctctcc ttctcgaagt cgccggtaac aagagagccg
      841 taaacatata gtccgacgag cgaaccgttc aagctccgcc gaatctcatc ggtcaggcga
      901 ccgacgagcg ccctgatctc atggtccggg gtgatttgca cagtccaagt gtagttgact
      961 ctcccaccgt gctctacccc gaccgggcct gtgcgtccgt ggacttcgtg gcgaggttcc
     1021 aacgctgaag tacaatgacg tttgtgaaca agggaactct ggatttctac gaactggtgg
     1081 agcgggcgct cgaagggttg ccgcccgagc tgtcggcact gctggataac gtggccatag
     1141 tagtggaaga ctggcccgat tactctacgc cgctggtggc ggatggtccg ggcgacacgt
     1201 tgtacgggct ctacgagggt gtgcctctga cggagagagg cgccggatac tacgggttcc
     1261 tgcctgataa gatcacgatc ttccgcggtc ctctggagcg ggacttccag accgacgagt
     1321 tggaggagca ggtcaggatc acggttgtac acgagatcgc gcactacttc ggctttgacg
     1381 aggagcgcct ggaggagctg ggttggggat gaactccggt gctgcgggaa gcgtccgcat
     1441 cgagcagcgg atagaggccc cgctcgagag ggtcgccgca tatatctcgg acttccgcaa
     1501 cgccagggag tggatggtcg gcgtcgaagc cgtcgagccg ctcggggagg aatccttccg
     1561 cctcaccctg gagagccctg taggcaggat cgaaccggcg gtgcgaattc tggagcgcga
     1621 ctcgcagagc atcagctggg tctacacatc agccatcgaa ggtggcggca gggtggatat
     1681 ctcacccgat accgacggtg gctgcctcgt cgcctacacc ggcgagttcc acctcaagcg
     1741 aaagttcctc gaccgcgccg cgcgcctggt cggcgtggag cgcttcgccc gctccaacgg
     1801 ggagcgctcc ctcaagcgcc tcaaacacct gatggaagcc aggcgctact gatgtccgtc
     1861 gggctgatcg agatcctgct cgtgttcatg gtgttcctgg tcctcggacc acgccgcatc
     1921 gcaaacttct tccgctcgat gggcaccggc atccacgact tcatggagac gctgggacgc
     1981 gataaagaca aggaacaact ccccggcgaa gacgacgagc acaaacgcaa cagctgaagc
     2041 ctacaaaccc agcaccaccc tgtcgcctgg gccgacgcgg gcattcggcg gattgcggtc
     2101 cacgccggag aggtcctcgc cagcgctcct cagatacacg cggggctcct cgacggcgag
     2161 gcccggatcg gcggcgtagg tctgccaggt gacgccctgc caggtaaagg tgaggagagc
     2221 agggggctca tccccgtcgt ccagctcgac gtagacctct ggctggccca gggttcggga
     2281 gagggcgttc atcgagtgcc gctgctctga ttcgttgaag aggtctatcc cggcccgcag
     2341 gcggttcgcc gggtctgcca ggtgcacgcg ctgttcccgc tcggcctcca gctcttcccg
     2401 cagcttcccg atggtcgtgc tgaggttgga gatctcaccc tccctctcct cgaggtctct
     2461 ctccaacccc tcgacgcggc ggagggcgtc ctggagcgcc aggtccttgc gcgaagccag
     2521 ctcctcggtc cgctggttcg cggcctccat ctgctcggcc ctgcgctcca gaaggtagtc
     2581 gcggtgtgag atctccccgt tcttgcgttc cagctcgcgc tccagacgcg ccacctggcg
     2641 ctcgaagctc tccctgcggc gcctgcgttc cctgacctcc tcttccaggt tcctgatgcg
     2701 ctccttcagc cttgcccgct cgctctcgag agactcgatg tgaccggccc cggcctcctt
     2761 cgttgcgcgg gcctcgtcgg cggcgagacg cgaagcggtg atctcctcgt atagctccct
     2821 gatgtggttg tccttgatca cgagcctggc ccttaactcc tcgggctctg agatctcccc
     2881 acgaccgccc tcgacctcgg tggtgtcggc accgtccggc cgctcgtcag acggctgcct
     2941 gccgttgtgt ccgtcctgac cttccatact cccctacccc tccttcttcg aggcctcctc
     3001 ttcagaatca ggatccggct gcgacgccgg ctgtttgagg gacttgccgg cctcccgcgc
     3061 cgcccgccgc tcggccttga tcctctccct ctcctcagcc gacatcttct tgggcttgtc
     3121 ctcgctccct gatgtttccg ctggcgcttc agtggtcgct gacgcgccgg tcctcaccga
     3181 tttgcccgcc tcgcgcgcgg ccctgcgttc ggctttgatc ctctcccgat cctcggccga
     3241 gagcttcttg ggagcctcct cggattcgga ggtactctcg cccgcctgtc cactcttgac
     3301 ggatttgccg gcttcgcggg cggcgcggcg ttcggccttt atcttctccc tctcctcagc
     3361 cgacatcttc ttcggcttct cgccgccggc ctcctctttc ttgggttcct ccggcttctc
     3421 tgcgggtttg gggaggccgg agatctcact tatcttcgcc agtatctcct cgggcgcggg
     3481 cctctcgccg acccacgcgt gggcgacctt gcggtctggc ccgacgacga agaccgcagg
     3541 gcgagagctg tcaccccgct tcctgtcctt atcgagcaat ccccactggc ggctcgcgac
     3601 ccgcgcatag tcgtagagca cgtaggactt cagcccggag gcgcgggcga agttacgtgc
     3661 ctgggctggc tcggccaccc cgatggcgac caccgagccc gctgccatgt tgatctcctg
     3721 ctccttcgcg gccagcgcct tgaagtactc cacgtcctct tcgtctcctg ggcggtagaa
     3781 cgcgaccacg acaggtccac gcgccgtcct catgctcaac ctgagctggc ctccctgcgc
     3841 gctagggagg ttgaactcag gggcatcagc ccccacctcg ggtacgggct cggtccttgt
     3901 gcgtgtgaac atccgttcct cctcgcgtgt aaaagaagcg cctgacccga atattactgc
     3961 aacaatgttt tcggtgtttt cctgctcaaa gtggcaggaa gcaaagtgcg gcagtctccc
     4021 gcgagacttt gcggtcatcg ccggagatac catcagcccc tacgaggtag ccccatgaac
     4081 tgtaacgaca ttgtacgcgt tcgcgtacaa actgtctcag gagacggacc tgggaagtcc
     4141 agaatagagt ccttcgcact tgggtgacga agagcttggg cgagttgccg cgtacgagct
     4201 cttccgtaac atcggtgccg tgctcctgtt cctccgcgtg gtgcattggt ccgatgtctc
     4261 ccttacacgc tgtgagtatc ctggagaatg gctgtgggcg atgcctcgga gcggcctgac
     4321 cctccttcca cgtgggcccc aagggtgcct tgcaagtgac tcgtcggccg gacgccttgg
     4381 cgtcgcccgc aggccgcccg gggccg
//
LOCUS       DATAIR010000013         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_184916, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000013 DATAIR010000000
VERSION     DATAIR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184916"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VHM69_00325"
     CDS             <1..368
                     /locus_tag="VHM69_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX2738860.1"
                     /translation="GPRDFPEGTITDYPESLVLAEYVREKALNVLREEVPHAVAVEIE
                     DVERKENVTVVYAVIHVERATQRMIVLGKGGRTIKKIGTEARRDVERLLGTRIYLDLK
                     VKVSPGWRSDRKFLERLGL"
     gene            413..1114
                     /gene="deoC"
                     /locus_tag="VHM69_00330"
     CDS             413..1114
                     /gene="deoC"
                     /locus_tag="VHM69_00330"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2738861.1"
                     /translation="MRVREFAKTVDHTLLKPDATQKEIVRLCEEASRYHFAAVCIPPC
                     YVPLAARELRGADVKVATVISFPFGYDATAVKIAAVREAVTRGASELDVVMNVSKFLS
                     GEFDYVADELASVTQEVSVVAMNNGLNDVVVKVIIETAYLSDKMKRLATQIVASSGAD
                     FVKTSTGFGPAGASPEDVALLREEAPESLAVKASGGIRTLGEALDILNAGASRLGMSA
                     SVAVMKEAESGGLQE"
     gene            1095..1844
                     /gene="recO"
                     /locus_tag="VHM69_00335"
     CDS             1095..1844
                     /gene="recO"
                     /locus_tag="VHM69_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2738862.1"
                     /translation="MAVYKSKGIVLRSIRYGEADRILDLYTMDAGLVSAIAKGIRRTK
                     SRFGARLEPLSCVDFVAYHGRTLDTVTQAETLRSFHGIREDLARFETAAGMVGCVRAL
                     SGGDEADRRVFNLLYKALDTLEGCDSGFDAIEAALGLKLSILAGYAPQLDVCLGCGTD
                     LANALEPLHFAPEHGGVLCRECRSATGDAFPIAPGTLESLRTLIEYPIREAPEHTGPR
                     ESVLRVVRSHVLAHAPGNTLIGPRLAGARPA"
     gene            1841..2818
                     /locus_tag="VHM69_00340"
     CDS             1841..2818
                     /locus_tag="VHM69_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX2738863.1"
                     /translation="MSAPESGLRRNPSAWAVEKSAGRQRPERPDGVRNDFQRDRDRII
                     HAKAFRRLMHKTQVFISPDGDHYRTRLTHTLEMMQISRTIARSLGLNEDLTEAISLGH
                     DLGHTPFGHTGEDALSRALSPHKRSFRHNEHSLRVVDHLEKGGEGLNLTFEVRDGIRN
                     HTGKGYPSTREGEIVRVADRIAYVNHDVDDALRAGIISRQDLPEGPISVLGVQMSARI
                     NTLVRDMISNSEANGEISLSDSVYEALMALRAWLFTHVYNNPGSRENQKAAGVIVALF
                     DYYLEHPEERADSDPDPITETIDFVAGMTDRYALAAYRRLFMPRGEIFA"
     gene            2898..3305
                     /locus_tag="VHM69_00345"
     CDS             2898..3305
                     /locus_tag="VHM69_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2738864.1"
                     /translation="MSSQELDELFAVAWSGHTPRDWELVLERSLVYICAYRESRLVGF
                     VNVAWDGSIHGFILDTTVHPEMRRRGIGRRLVLRAASEARDRRVEWLHVDFEPHLRNF
                     YDSCGFRATEAGLMHLASGGRRAESNSAGVQHP"
     gene            complement(3282..>5006)
                     /locus_tag="VHM69_00350"
     CDS             complement(3282..>5006)
                     /locus_tag="VHM69_00350"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX2738865.1"
                     /translation="MSERLRRTLQRSDSLTAYPGVKDFVDDLSTIRDSLMNNGGERVA
                     EGALRDLIRQAEVFGFHLARLDVRQESATVVKAAAELISAGTGEDLLEMEEAARAAHL
                     RKLLADPELLLPEPQDISEESRKVIETFERIRRAKEDFSGTPVETFVLSMAHHASDVL
                     SVQLLARRAGLLEVDESGRCTANYLRVTPLFETIDDLQRAPEVLRFLLEDPFYRSSLS
                     RSDDLQEIMLGYSDSGKDSGYVTSNWTLYKAQGSLCSVASSFGVKLRLFHGRGGSAGR
                     GGGPSYQAIMAQPPDTLHGRIRITEQGEVISFKYSIRGLARRNLDTVLAAVLEASADN
                     TPRDPEPRWIEVMEELSATAREAYRDLVYEDEGFLDFFSEASPIGELSLLNMGSRPAR
                     RMQNPEVESLRAIPWVFAWTQNRFLLPSWYGAGSAFRSYAAEEHGLEVLREMYESWPF
                     FRTLIDFMQMTLAKSDLRIARNYTSLVSSPATRERLWTRISDEHAACTEAILLVTGNE
                     NLLDDSPVLQRSIRLRNPYVDPLSYVQVTLLRRLRALPEDSPERERVLNILLLTISGV
                     SSGMLNTG"
BASE COUNT          960 a         1519 c         1596 g          931 t
ORIGIN      
        1 ccggacctcg cgacttccct gaaggaacca tcactgacta ccccgagtcg ctggtcctcg
       61 ccgagtacgt gcgggagaag gcgctgaacg tcctgcgcga agaggtcccc catgcggtgg
      121 ccgtcgagat cgaagacgta gaacgcaagg agaacgttac ggtcgtctac gccgtcatcc
      181 acgtcgaacg cgcgactcag cgcatgatcg tgctcggcaa aggcggcagg accatcaaga
      241 agatcgggac cgaggcccgc cgcgacgtcg agcgcctgct cggcacacgt atttacctcg
      301 acttgaaggt aaaggttagt ccgggatgga ggagcgacag gaagtttctg gagcggttgg
      361 gtttataatc acgctgcgtc gtcgttgagt cgagaggaga gagacgttgg ctatgagggt
      421 gcgggagttc gcgaagacgg tggaccacac cctcttgaag ccggacgcga cgcagaagga
      481 gatcgtgcgt ctgtgtgagg aagcgtcccg gtaccacttc gccgcggtgt gcattccgcc
      541 gtgctacgtc ccgctcgccg ccagggagtt gcgtggggca gacgtgaagg tggcgacggt
      601 gatctccttt cccttcgggt atgacgctac ggcggtcaag atagcggccg tgcgcgaggc
      661 ggtcacaaga ggagcttccg agctcgacgt ggtcatgaac gtctcgaagt tcctttcagg
      721 cgagttcgat tacgtcgccg acgagctcgc cagcgtcacg caggaggtca gcgtggtcgc
      781 catgaacaac gggttgaacg acgttgtggt gaaggtcatc atcgagacgg cgtacctctc
      841 ggacaagatg aagcggcttg cgacccagat cgtcgcttcc agcggcgcgg atttcgtgaa
      901 gacctcgacc ggcttcggcc ccgccggggc cagccctgag gacgtagccc tcctcaggga
      961 ggaggcgccc gagagcctgg cggtgaaggc gtccggtggg atacgtaccc tgggagaagc
     1021 cctggacatc ctcaacgccg gggcctcgcg tctcggcatg agcgcgagcg tagccgtaat
     1081 gaaagaggcc gagagtggcg gtctacaaga gtaaagggat cgtcctgcgc tcgatccgct
     1141 acggtgaggc ggatcggatc ctggacctgt acaccatgga tgccggtctc gtctcggcta
     1201 ttgccaaggg catccgacgg acgaagtcac gcttcggcgc ccgcctcgaa ccattgtcct
     1261 gtgtagattt cgtggcgtac cacggccgca cgctcgacac cgtgacgcaa gccgagacgc
     1321 tgcgtagctt ccacggcatc agggaagacc tcgcccgctt cgagaccgcc gccggtatgg
     1381 tcgggtgcgt gcgcgccctc tcaggcggcg acgaggccga ccgccgtgtc tttaacctgc
     1441 tgtacaaagc gctcgacacg ctggaaggat gcgattctgg tttcgacgcc atcgaggcag
     1501 ccctcggcct gaagctctcg atcctggcgg gctatgcgcc gcaactcgac gtctgcctgg
     1561 gctgcggcac ggacctcgcc aacgctctgg aaccacttca cttcgccccc gaacacggag
     1621 gcgtcctgtg cagggagtgc aggtcggcca caggcgatgc tttccccatc gcccccggca
     1681 cgctcgaaag cttgcgaacc ctgatcgaat accccatcag ggaagcaccc gaacacacag
     1741 gacctagaga gagcgtcctg cgggtcgtac gctctcacgt cctcgcccac gctcccggaa
     1801 acaccctgat cggaccccgc ctcgcaggcg cgcgcccggc atgagtgccc ccgagtccgg
     1861 cctcaggcgg aatccttcgg cgtgggcggt ggagaaaagc gccggacgcc agaggcccga
     1921 gcgccccgac ggtgtcagga acgacttcca gcgcgaccgg gaccgcataa tacacgccaa
     1981 agcgtttcgc cggctgatgc acaagaccca ggtcttcata tctcccgacg gcgaccatta
     2041 ccggacacgc ctgacccata ctctggaaat gatgcagatt tcacggacga tagcccgctc
     2101 tcttggcctc aacgaagacc tgacagaggc catctctctc ggccacgacc ttggccacac
     2161 ccccttcggg catacaggtg aggatgccct ctcccgcgcc ctctctcccc acaaacgctc
     2221 cttccgccat aacgaacact ccctgcgcgt agtggaccac ctcgagaagg ggggagaagg
     2281 attaaacctc acattcgagg tcagagatgg gatacgtaac catacaggaa agggctatcc
     2341 ctccacgcga gaaggtgaga tcgtgcgcgt cgctgaccgt atcgcgtacg taaaccacga
     2401 cgtggacgac gcattgcggg cggggatcat ctctcgacag gacctcccgg agggcccgat
     2461 ctcggttctc ggagttcaga tgagtgccag gattaatacc ctcgtacgag atatgatctc
     2521 taactctgaa gcaaatggtg agatatctct ttctgattcg gtgtacgagg cgttgatggc
     2581 gttacgggcg tggcttttca cgcacgtgta caacaatcct ggctcgcggg agaatcagaa
     2641 ggcggcaggt gtgatcgttg cgctcttcga ctactacctg gagcatccgg aggaacgggc
     2701 ggacagcgat cccgacccca tcaccgagac catagatttc gtagctggga tgaccgaccg
     2761 ctacgctctc gcggcttaca ggcggctctt catgcccaga ggcgagatct tcgcgtgacg
     2821 atgctcatta tctggaaaca cgtttgaaag cacgccggtc cgatgaactt gacatcgtct
     2881 atcgtgtcga tcctcctgtg agttcgcagg aactcgacga gctgttcgcc gtggcgtggt
     2941 cggggcacac gccgcgcgac tgggagctcg tgctcgaacg tagcctggtg tatatctgtg
     3001 cgtataggga gagtcgtctc gtcgggttcg tcaacgtcgc gtgggatggg agtatccacg
     3061 gattcatact cgacaccacg gtccatcctg agatgcgccg gcgcggtatc ggacgcaggc
     3121 tcgtcttgcg ggcggcgtcc gaagcgaggg accggagggt ggaatggcta cacgttgact
     3181 tcgagccgca cctgaggaac ttctatgata gttgcggctt cagggccacc gaggcagggc
     3241 tgatgcacct ggcaagtggc ggccggcgcg ccgagagcaa ttcagccggt gttcagcatc
     3301 cctgaggaga ctccagagat agtaagcaac aagatattca gaacgcgctc ccgctccggg
     3361 gagtcttccg ggagggcgcg gagccgacga agtagcgtga cctgaacgta ggagagcggg
     3421 tccacgtaag ggttacgaag gcgtatggat ctctggagca cggggctgtc gtcgaggagg
     3481 ttttcgttcc cagtaacgag caagatcgcc tcggtacagg cagcgtgttc gtcggagatc
     3541 ctggtccaca gccgttcgcg tgtggccgga gacgagacga gcgaggtgta gttcctggct
     3601 atgcgcaggt cgctcttcgc cagggtcatt tgcatgaagt cgatgagcgt gcggaagaaa
     3661 ggccaactct cgtacatctc gcggaggacc tcaaggccgt gctcctccgc cgcgtagctc
     3721 ctgaaggcgc tacctgcgcc gtaccaggat ggtagaagga agcggttctg ggtccaggcg
     3781 aagacccatg gaatcgccct caggctctcc acctcggggt tctgcatcct tcgcgcgggc
     3841 ctgctgccca tgttgagcag ggagagctca ccgattgggg aagcttcgga gaagaagtcc
     3901 aggaagccct catcttcgta gacgaggtcc ctgtaagctt ctcttgccgt cgctgacagc
     3961 tcttccatga cctcgatcca gcggggttcc gggtcgcggg gggtattgtc tgcgctcgct
     4021 tcgaggacgg cggcgagcac ggtatccagg ttacggcggg ccaggccgcg gatgctgtac
     4081 ttaaaggaga tcacctcacc ctgctcggtt atcctgatcc tgccatgcag ggtatctggg
     4141 ggttgggcca tgatcgcctg gtagctaggc cctccacctc gtccggcgct gccgccgcgc
     4201 ccgtggaaga gccgcaactt cacaccgaag ctgctggcca ccgagcagag cgaaccctgc
     4261 gccttgtaca gggtccagtt gctcgtgacg tagcccgagt ccttgcccga gtcactgtag
     4321 ccgagcatta tctcctgcag gtcgtcgctc cgggagagcg aagagcggta aaaggggtct
     4381 tccagcagga agcgcagcac ctcaggcgcc cgctggaggt cgtcgatggt ctcgaatagt
     4441 ggcgtgaccc tcaagtagtt ggcggtgcac cgtccactct cgtcaacttc caacagcccg
     4501 gcccggcgag ccaggagttg cacgctcagt acgtcactcg catggtgggc catgctaagg
     4561 acgaacgtct cgaccggggt gccggagaag tcttccttcg cgcgccggat gcgttcgaag
     4621 gtctcgatca ctttgcgcga ctcttccgaa atgtcttgcg gttcgggaag tagcagctcg
     4681 gggtccgcga ggagcttgcg caggtgtgcg gcccgcgcgg cctcctccat ctccagaagg
     4741 tcttcgccgg tgccggcgga tatcaactcg gcggcagcct tcactaccgt tgcgctctcc
     4801 tggcgcacgt ccagcctggc caggtggaag ccgaagacct cggcctggcg aatgagatcc
     4861 ctcaaagccc cttcggcgac gcgctccccg ccgttattca tcaggctgtc gcggatggtc
     4921 gaaaggtcgt ccacaaagtc cttcacgcct gggtaggctg tgagggagtc tgatctctgc
     4981 agcgtgcgtc tcaggcgctc ggacat
//
LOCUS       DATAIR010000014         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416116, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000014 DATAIR010000000
VERSION     DATAIR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416116"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VHM69_00355"
     CDS             complement(<1..1145)
                     /locus_tag="VHM69_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX2738866.1"
                     /translation="MTVALVNLWRVVRNARVRLFGRPPEFVWIEASGSLPEFETPVGF
                     LRRRFAPGPSPPTLERLRGQLEHIFADGRPRGVILRIKNLDAGWAALEELRREILGFR
                     ARGARVVAYFADSVDIRSYYLACAADEILATPLATLNVVGLRARVDFLKDAMDKLGLE
                     AEVVAVSPYKSAGERFVRNDFSRESREQVERLLDRRYEEVVAGVSEGRDLSPEEVSAR
                     IDLAPYGAQEALSNGLIDGVGYEDELPERLGQDDGRAGLAEWGQARRSLRMPYRRRTR
                     KRVALITLSGTIVRGRSRKLPVPLPFIGGEQAGSESVVAALRVAEKNRRIASVLLHVD
                     SPGGDALASDLIWREIERLRDKKPVVVLMGNAAASGGYYVSAPASHVV"
     gene            1256..1678
                     /locus_tag="VHM69_00360"
     CDS             1256..1678
                     /locus_tag="VHM69_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX2738867.1"
                     /translation="MLDPDIREKLRRSGYTLTSQRRAILDALSEAKGHPSAEDVYLIV
                     KRKNPRVALGTVYQALAVLEKVGVIGSKHWAESPTRYDLNVEPHLDIRCTRCGEVSEV
                     PGIALDDIEERIRNNTPYRVTRATMVVEGVCPECAENE"
     gene            1859..3700
                     /locus_tag="VHM69_00365"
     CDS             1859..3700
                     /locus_tag="VHM69_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738868.1"
                     /translation="MSEDPRETAAGLAEGMEAASALIESLEQEVENLRGDLEQAAVAL
                     KAAQNEVSSRAGALEEKERARLSAEREVEDLRAEVSELRQRHSDEQLRLSNAHINELA
                     EVRRRLEEQRRADVDAASSETRLDSLKAEFRREREALEARYREEIESLRNASEQWEEQ
                     LRSSYQEQEARHSTELESARRSADERTNSLERSLREEYEHRLAEERSASDERQTAAVQ
                     ALRNAAAGRELELQKDYQAVVERQQEEIESLREELESARLASEEQLNNDLRAVKALAE
                     RRENDLRKAQAARLAEAKESADRRVAAIQAQREADNKALRARHAEQTAQLRREHEERL
                     AAEDERRKQETWALDERLREATVQRETELRTYSARLKELGAARLAQKSSSAADLERVV
                     ERFGAEISEYEGRITALEETLAESEARRNDLETVIDELRSGQGGAEESVTGREGPDRA
                     EDEQEGRLREVEAQRILAEEKVQDLEARLREAREESRRNAEGLQDALERLNRLSEPER
                     RLRQGIALFNESEHARTVASISKALGLPKVHAALDDTSPGKPTLTFLWGDMAWRRYVS
                     DPTEGVEEPRVYLTGTGDDSAEVEASSRQPNARMDSKGRLMIGVQAR"
     gene            complement(3697..4146)
                     /locus_tag="VHM69_00370"
     CDS             complement(3697..4146)
                     /locus_tag="VHM69_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738869.1"
                     /translation="MRSVVAVGIGVLLALVLGLLLVQGIFAPVFTRFFGLERTGPETL
                     PVVLLVFAAAFSFYFGGMAASYKAPSRHRLHGVLVVPAAFFLSTGLNFLTGKGFLPGV
                     DGTGAVALVAVFLAVSAAAAFVGGHRGEALYVHNKKVAQRLRRQPPD"
     gene            4224..>4754
                     /locus_tag="VHM69_00375"
     CDS             4224..>4754
                     /locus_tag="VHM69_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2738870.1"
                     /translation="MKRRVVPSCTVRRRTLTRRDFLKLTGAGMAGATLLGATGCDLTE
                     RIRNIKNDRPSLKEGTNVVLVIIDSLRKDHIGAYGNDTIHSPNLDALAKESLRFTQAY
                     PESIPTICARRAIHTGRRTWPFKDWKPPKGEDIILQGWEPIPNDQVTLAEMMRAAGYG
                     TYFVTDNMHQFKPSYNM"
BASE COUNT         1038 a         1511 c         1551 g          654 t
ORIGIN      
        1 acgacgtggc tcgccggtgc cgagacgtaa tagcctcccg aggccgctgc gttccccatc
       61 aggacgacga cgggtttctt atcgcgcagg cgttcgatct cgcgccagat caaatcggaa
      121 gccagcgcgt cgccgccggg cgaatctacg tggagcagca ccgacgcgat cctcctgttc
      181 ttctcggcaa cccgcagcgc cgcgacgacg gactcgctcc cggcctgctc ccctccaatg
      241 aagggcaacg gcacaggaag cttcctgctc ctgcccctca ctatggtccc tgacagggtg
      301 atgagcgcga cccgcttgcg cgtccttctc ctgtagggca tacgtaggga ccttcgcgcc
      361 tggccccact ccgcaagccc ggcccgcccg tcgtcctgcc cgagtctctc aggcaactcg
      421 tcctcgtaac cgactccatc gatcagtccg ttcgagagtg cttcctgcgc gccgtacggc
      481 gccaggtcaa tcctggcact cacctcctca ggggagaggt ctctgccttc cgagaccccg
      541 gccacgacct cctcgtagcg gcggtcgagg agacgctcga cctgctcgcg ggactcgcgc
      601 gagaagtcgt tgcggacgaa acgttcaccc gcagatttat aaggagagac ggcgacgacc
      661 tccgcttcga gaccgagctt gtccatggcg tctttgagga aatccaccct ggcgcggaga
      721 ccgacgacgt tcagcgtggc gagcggggta gccaggatct catcagccgc gcaagcgagg
      781 tagtatgagc gaatgtcaac ggagtcggcg aagtaggcta ctacgcgggc tccccgtgcg
      841 cgaaagccca ggatctcccg tcgcaactcc tccagggcgg cccacccggc gtcgaggttc
      901 ttgatgcgga gaatgacgcc gcgcggacgg ccatcggcaa agatatgttc gagttgcccg
      961 cgcagccttt ctagtgtggg cgggctcggg ccgggagcga atcggcgtcg aagaaagcct
     1021 acaggcgtct cgaactcggg caacgaacca ctagcctcga tccagacgaa ctccggagga
     1081 cgcccgaaca ggcgcacgcg ggcgttccgc accacgcgcc agaggttcac cagagcgact
     1141 gtcaacagcg acatctggca gctatagtac accgcggcag gaaaaacgcc gtatgataaa
     1201 ctcgcaaaca ggaatggtta ctgatcgtcc atcaacgcag agagcttctt caggcgtgct
     1261 cgaccccgac atacgggaga agctgcgcag gtcgggctac accctcactt ctcagcgcag
     1321 ggccatcctc gacgctctct ccgaagcgaa gggacacccc tccgctgagg acgtgtacct
     1381 gatcgtcaaa cgaaagaacc cgcgcgtggc gctaggcacg gtctaccagg cgctcgccgt
     1441 actcgaaaag gtcggcgtca taggctccaa gcactgggcg gagtctccca cgcgctacga
     1501 cctcaacgtg gaaccacacc tggatatcag gtgcaccaga tgcggtgagg tctccgaggt
     1561 ccctggcatc gcactcgacg atatagaaga gcgaatccga aacaacactc cttacagggt
     1621 cacccgcgcg acgatggtcg tcgagggcgt gtgccctgag tgcgccgaga acgaataacc
     1681 gaagcacagg tcccggcagt ccgtacggtt ctcgttcgac ttcgttccca ggcaaacgtt
     1741 gctcccgccg gggatgagag gtcccgatct cacctgtaca caggggcttt accgcgtgcc
     1801 tgccaggagt taccctattt accagcgtgg ttccgaggga aataggggaa gggcgatcat
     1861 gagcgaggat cccagggaga cggcggccgg gctcgctgag ggcatggaag cggccagcgc
     1921 cctgatcgag agcctcgagc aggaggtgga gaaccttcgg ggcgacctcg agcaggctgc
     1981 cgtcgcgctg aaagcggcgc agaatgaggt ttcgtcccgt gccggtgcgc tggaggagaa
     2041 ggagcgggcc cgcctcagcg ccgagcggga ggtcgaggac ctgagggcgg aggtctctga
     2101 gctcaggcag cggcactccg acgagcagct ccgcctcagc aacgcgcaca tcaacgagct
     2161 cgcagaggtg cgccgcaggc tcgaagagca gcgcagggcc gacgtggatg cggcctcctc
     2221 ggagacgcgt ctcgacagcc tcaaggcgga gttcaggcgc gagcgggaag ccctcgaggc
     2281 acgctacagg gaagagatcg aatccctgag aaacgcctcg gaacaatggg aagaacagct
     2341 gaggagtagt taccaggaac aggaagcccg ccactcgacc gagctcgagt cagcaaggcg
     2401 ttcggcggac gagcggacga actccctgga gcgttccctg agggaagagt acgagcatag
     2461 gctggccgag gagcggagcg cctcggacga gcgtcagacg gcggcggtgc aggcgctcag
     2521 gaacgccgct gccggaaggg agctggaact ccagaaagac tatcaggcag ttgtcgaaag
     2581 gcagcaagaa gagatagagt cgctgcgcga agagctggaa tcagcccgcc tggcttcgga
     2641 agaacaactc aataacgacc tgcgggcggt caaggcgctc gcggagaggc gtgagaacga
     2701 cctgcgaaag gcgcaagcgg ccaggctcgc ggaggcgaag gagtccgcag accgaagggt
     2761 cgcggcgatc caggcccaga gggaggcgga caacaaggcg ctcagggcgc gccacgctga
     2821 gcagaccgcg cagctgcggc gcgagcacga ggagcggctc gccgccgagg acgagcgccg
     2881 gaaacaggag acctgggcac tcgacgagcg gctaagggag gccaccgtcc agcgcgagac
     2941 ggagctgcgc acgtactcgg cacgcctgaa ggagctgggg gccgcaagac tcgcccagaa
     3001 atcttcatcc gcagcggatc tggagcgggt ggtcgagcgg ttcggggctg agatctccga
     3061 gtacgagggc cgtatcaccg cgctggaaga gaccctcgcg gagtccgagg cacgcaggaa
     3121 cgacctggaa acggtcatcg acgaactgcg gtcgggccag ggcggcgcgg aggaatccgt
     3181 aaccggacgc gaaggccccg atcgcgccga ggacgagcag gaagggcgtc tgcgggaggt
     3241 cgaggcacag aggatactcg ccgaagagaa ggtgcaggac cttgaagcca ggctcaggga
     3301 ggccagggag gagagcaggc gcaacgccga agggctacag gatgccctcg aacgtctcaa
     3361 caggctctcc gagccggaac gcaggctgag gcaggggatc gcactgttca acgagagcga
     3421 gcacgcgagg actgtcgcct ccatctccaa ggcactcggc ctcccgaagg tgcacgccgc
     3481 cctcgacgac acgagccctg gcaagccaac gctcacgttc ctctggggcg acatggcctg
     3541 gcgccgctac gtctcggacc ccacggaggg agtcgaggaa ccgagggtct acctaacggg
     3601 cacgggcgac gactcggcag aggtagaggc gtcgagccgg cagcccaacg ccaggatgga
     3661 ctcgaagggg aggttgatga tcggcgtcca ggcccgttag tcggggggct gacggcgaag
     3721 ccgctgcgcg actttcttgt tatggacata gagcgcctca ccgcggtgac cgccgacgaa
     3781 cgcggccgcc gccgaaaccg ccaggaagac ggccacaagc gccacggcac cggtcccgtc
     3841 gacgcctggc aggaacccct tacccgtaag aaagttcagt cctgtcgaga ggaagaaggc
     3901 cgcggggacg acgaggacgc cgtgcagacg atgcctcgat ggcgccttgt agctcgctgc
     3961 catccccccg aagtagaaag agaaggcggc cgcgaagacg agcaagacca cgggcagggt
     4021 ctcggggccc gtccgctcca atccgaaaaa gcgcgtgaag accggcgcga atatcccctg
     4081 caccagcagt agaccgagca cgagcgcgag caacaccccg atgcccacag ccacaacgct
     4141 tctcataaaa actattctag caggcccctc acagagtcca cgtagccgcc ttcaccgaca
     4201 tacctccccc tgaggcttgt agggtgaagc gccgcgtagt accctcttgc accgtgagac
     4261 ggcgtacgct aacccgcaga gatttcctca agctgaccgg cgccgggatg gccggggcga
     4321 cgctgctcgg tgcgacgggc tgcgacctca ccgagcgcat acgcaacatc aagaacgatc
     4381 gtcccagtct caaagagggc acaaacgtgg tactcgtcat catcgacagc ttgaggaagg
     4441 accacatcgg cgcctacggc aacgatacca tccacagccc caacctggac gcccttgcga
     4501 aagagagcct gcgcttcacc caggcatacc ccgagtccat accgacgatc tgcgcgcgga
     4561 gggccataca cactggcaga cgcacctggc cattcaagga ctggaagcca cccaaaggcg
     4621 aggacataat cctgcagggc tgggagccaa tacccaacga ccaggtcacc ctcgccgaga
     4681 tgatgcgcgc ggcgggttac ggtacctact tcgtaacgga taacatgcac cagttcaagc
     4741 cctcgtacaa catg
//
LOCUS       DATAIR010000015         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416117, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000015 DATAIR010000000
VERSION     DATAIR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416117"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..694)
                     /gene="ispD"
                     /locus_tag="VHM69_00380"
     CDS             complement(11..694)
                     /gene="ispD"
                     /locus_tag="VHM69_00380"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX2738871.1"
                     /translation="MLAGGTGSRMGHPKQFVDLLGRPALHYTLRAFEDTPEVARIYTV
                     GDRLRIEALVQTAGISKYAACAEPGDFRSFSTRNGLLLCEEDPETIVLVHDGSRCLVT
                     PDLIRRVVAAAGGGPDGVVPGVLVSDTIKVAREDGTVSDTLDRTNLRAIQTPQAFRLG
                     LLRRLHDSPEEYLRAATDDASLVEREGGSVEIVAGEKTNIKLTYPEDLVFAEAILVAR
                     EGATHEVWP"
     gene            complement(754..1839)
                     /gene="disA"
                     /locus_tag="VHM69_00385"
     CDS             complement(754..1839)
                     /gene="disA"
                     /locus_tag="VHM69_00385"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX2738872.1"
                     /translation="MSSQRRLLPAELAGALDLVSPGTDLREAIENIIRAHNGALIVVN
                     RPEKLEDLGIISGGMRVELEFAPMRLYELAKMDGAIVVSPDISAIHYANVQLSPDPNL
                     DSEETGMRHLAAHRTAQQTGGLVIAVSERRRVVSLYQGSYGPHVLEDIGVVLSKANSA
                     IATLEKFTRRLREEARGLTVHEYDGAVTLKEVISAVGTFEYSTRIAEEIQAYVRELGR
                     EGRLVEMQLEQAFHGVPEQYEALLRDYVAEGVDYKDVRADLRTFSSDQLSEPAEIARA
                     LGYTSVGQMEDFFIKPRGYRQLERVPRLPRRVAESLIKEFGSLKELLDATEEELDEVE
                     GVGQARARAIQRGLERQRSLEFTGEVS"
     gene            complement(1836..3200)
                     /gene="radA"
                     /locus_tag="VHM69_00390"
     CDS             complement(1836..3200)
                     /gene="radA"
                     /locus_tag="VHM69_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX2738873.1"
                     /translation="MATKTLYVCSNCGHSEPKWLGRCPDCGEWSTFVEEVREEKKVVG
                     FAERATRRKGRVGGRTLALSDVPVVEGGGRMDVGIGELNRVLGGGILPGSMVLVGGEP
                     GVGKSTLLLQVMGHLGEGCLMVSGEESPRQVALSARRLGVEGKGFRVLSETDVDVIEA
                     TILEERPRIVVVDSIQTLYSSELSGAPGGVGQVRECAARLMRLAKSEGIAVVLVGHVT
                     KDGSIAGPRVLEHMVDTVLQFEGDRFQSFRVLRALKNRFGSTNEVGVFEMTDRGMVEV
                     EDPSAFFLSNREEEMPPGVVTVCLLEGTRPMLVEIESLVAPSPLANPRRIANGVDTGR
                     VNMLCAVLSRRAGLALGDQDVYVNVTGGVRVEEPAADLGVALAIASAMRDKPVGKGTA
                     CFGEVGLTGDVRYVSGAQRRTSEMVKMGFSRIIRPEGTSDGRGEASVVEVSTLEEAVA
                     VALL"
BASE COUNT          541 a         1189 c          938 g          591 t
ORIGIN      
        1 ccgacacgca tcagggccag acctcatggg tggctccctc gcgcgcgacg agtatggcct
       61 cggcgaacac gaggtcctcg ggataggtca gcttgatgtt ggtcttctct ccagcgacga
      121 tctccacgct gccaccctcc ctctcgacca gtgaggcgtc gtccgtcgcg gctctcaggt
      181 actcttccgg agagtcgtgg aggcggcgca acaggccgag gcggaaggcc tggggagtct
      241 ggatcgcacg caggttcgtc ctgtcgaggg tatcggagac cgtgccgtcc tctctcgcga
      301 cctttatcgt gtcagagacg agaacccctg ggacgacgcc gtcgggccct ccgcctgctg
      361 ctgctacgac gcgccttatg agatcaggcg tcaccaggca gcgggagccg tcgtgcacga
      421 ggacgatggt ctccgggtcc tcctcgcaga ggagcagacc gttcctggtc gagaaggagc
      481 ggaagtcgcc aggctccgcg caggctgcgt acttgctgat gcccgctgtc tgcacgaggg
      541 cctcgatcct gaggcggtcg ccgaccgtgt agatcctggc gacctccggg gtatcttcga
      601 aggcgcgcag tgtgtagtgg agggcgggcc tgccgaggag gtcgacgaat tgcttcggat
      661 ggcccatcct gctgcctgtc ccgccggcga gcacgagcgc gatcgctgat cccctctccg
      721 ctatctcggg ccgccctccg aagcctccac gccctacgac acctcgcccg tgaactccag
      781 gcttcgctgg cgctccaggc cgcgctggat cgcgcgggcg cgcgcctggc cgacaccctc
      841 cacctcgtcc agctcttctt ccgttgcgtc gagcagctct ttgagggagc cgaactcctt
      901 gatgaggctc tccgcgactc tgcgcggcag acggggcacc cgctctagct ggcggtagcc
      961 ccttggtttg atgaagaagt cttccatctg gcccaccgag gtgtatccga gggcgcgggc
     1021 gatctccgcc ggctccgaga gctgatccga agagaaagtc ctcagatccg cccttacgtc
     1081 cttgtagtcc acaccttcgg cgacgtagtc cctgagcagt gcttcgtact gctcgggaac
     1141 gccgtggaag gcctgttcga gctgcatttc gaccagccgg ccctcgcgcc cgagctcccg
     1201 cacgtaggcc tgtatctcct ccgcgatgcg ggtcgagtac tcgaatgtgc cgacagcgct
     1261 gatgacctcc ttcagcgtca cagccccgtc gtactcgtgc actgtcaggc cacgcgcctc
     1321 ctccctcagc ctccttgtga acttctcgag cgtcgcgatg gccgagttcg ccttcgaaag
     1381 cactaccccg atgtcctcga gaacgtgcgg cccgtaggag ccctggtaaa ggctcaccac
     1441 cctccgtcgc tcggagaccg ctatgacgag gccgcccgtc tgctgcgccg tgcggtgcgc
     1501 cgcgaggtgg cgcatcccgg tctcctcgga atccagattg gggtctggac tgagctggac
     1561 gttcgcgtag tggatggcgg agatatccgg cgagaccacg atggcgccgt ccatcttcgc
     1621 cagctcgtag aggcgcatcg gcgcaaactc cagctcgacc ctcatccccc ccgagatgat
     1681 gccgagatcc tcgagtttct cgggtctgtt gacgacgatc aaggctccgt tgtgcgccct
     1741 gatgatgttc tctatggcct ccctgagatc cgtgcccgga gacacgagat cgagggcccc
     1801 cgccagctcc gcgggcagga gtcgcctttg agaactcata acagcgccac cgccacggcc
     1861 tcctccagcg tactcacctc caccacgctc gcctctcccc tgccatccga tgtaccttcc
     1921 ggcctgatta tacggctgaa gcccatcttg accatctcgg aggtccggcg ctgcgccccc
     1981 gagacgtagc gcacatcgcc cgtgagcccg acctcgccga aacaggccgt acccttgcca
     2041 acaggcttgt cccgcatcgc cgatgcaatg gcgagcgcca cccccaggtc ggccgcgggc
     2101 tcctcgaccc gaacgccgcc agtaacgttg acgtaaacgt cctgatcccc tagcgccagg
     2161 ccagccctcc tgctcagtac cgcacacaac atgttgaccc gtccggtgtc caccccattg
     2221 gctatccggc gcgggttggc gagcgggctc ggggcgacga ggctctctat ctcgacgagc
     2281 atgggccgcg tcccttcgag caggcagacc gtgacgaccc caggcggcat ctcctcctcc
     2341 ctattcgaga ggaagaacgc cgaagggtcc tcgacctcga ccatcccgcg gtccgtcatc
     2401 tcgaagaccc cgacctcgtt ggtcgacccg aagcggttct tgagcgcccg gagcaccctg
     2461 aagctctgga acctatctcc ctcgaactgg aggaccgtat ccaccatgtg ctcgaggacg
     2521 cgggggcctg cgatcgaacc gtccttcgtc acgtgcccga cgaggacgac cgctataccc
     2581 tcgctcttgg cgagccgcat gagcctggct gcgcactcgc gcacctgtcc gacaccgccc
     2641 ggcgcccccg agagctcgga cgagtagaga gtctggatcg aatcgaccac cactatcctc
     2701 ggcctctctt ccaggatagt cgcctcgatc acatccacat cagtctccga gaggacccgg
     2761 aagccctttc cctcgacccc gaggcgcctc gcgctcagag cgacctgcct gggcgattcc
     2821 tcccccgaga ccatgaggca gccttccccc agatgtccca tcacctgcaa caggagcgtg
     2881 ctcttcccga cgccgggttc tcccccgact aggaccatcg agccaggcag gatcccacct
     2941 cccaggaccc ggttcaactc gcctatcccc acgtccatcc ggcccccgcc ttcgacgaca
     3001 ggcacgtcgc tcaaggccag cgtccgccct ccaaccctcc ccttccgcct cgtcgcccgc
     3061 tcggcgaagc cgacgacctt cttctcctcc cttacctcct caacgaaggt gctccactcc
     3121 ccgcagtcag ggcaacgccc gagccacttc ggctcggagt gtccacagtt ggagcagacg
     3181 taaagtgtct tcgtagccat gtgtcaattg tacgagatcg taatcagagt acagcaccaa
     3241 acggaagccg ggacgccat
//
LOCUS       DATAIR010000016         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_677, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000016 DATAIR010000000
VERSION     DATAIR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VHM69_00395"
     CDS             complement(<1..285)
                     /locus_tag="VHM69_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738874.1"
                     /translation="MTVTEARTPGEIQEKTAGTGRRRLGNWLAFAVLLVAAIIWLIPL
                     AWAVDTALKTEADTTAIPVSWIPPSGFTFESFAEVLSAGNLLRWYFNSALT"
     gene            complement(285..1214)
                     /locus_tag="VHM69_00400"
     CDS             complement(285..1214)
                     /locus_tag="VHM69_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2738875.1"
                     /translation="MAARAETAQQTRAKGERERRIDLTGYLFVLPFLIAYALFLVWPI
                     ILGLRMSFFDWSLVGTGTRDFLGLANFRELFSDPDFWASLWHTILFTLLSTPPLVLLA
                     LGLALLANRAVPARWFFRVAFFAPYVLAPSVVALIWVWLYQPGFGLINSYLVSLGLPE
                     VDWLAGENVAMISVVIATVWWTIGFNFVLYLAGLQEIPEELYEAAALDGAGGFAQTRY
                     ITIPLLSRTTFLIVVLQILASLFVFDQIYIMNTSGGLNYTVTRPIIQYVYEQGFTQYR
                     VGFAAAMSYVFFVLILVFNITQFVLARRRRREA"
     gene            complement(1245..2582)
                     /locus_tag="VHM69_00405"
     CDS             complement(1245..2582)
                     /locus_tag="VHM69_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2738876.1"
                     /translation="MDRGQIGTGAKAVRRVSRRRFLYGAATALGGMALASCSGGPFGS
                     SKQKVHFWNLFGGGDGARLADMESDFIQSHPEIDLSATTLTWGAPYYTKVSMATVGGR
                     PPDVAVMHLTRLPAYAPAGLLEPLAPDVLAEYDIGQDKFLPEIWEGGKYNGEIYAIPL
                     DTHPQVMFYNTDICKEAGLLDSDGNLKPLQGRDELIDAFQKSMEVTKEQGIAFAPADP
                     VVPWRLFYSFYGQLGGEVLSPDASEVILDQEKAEEALSFLVELTLDKEFTPSNQDYGA
                     AVAQFQSGNAGFHWNGEWEVTTFLDAKMPFSMTEFPDVFGNKHVQADRHTFVIPKGIA
                     NDSKAFDGALKFISSMLKSSLIWAEGGHIPAYRSVLESSEYKNLKPQSNYAGVADKVV
                     LDPKAWFSGSGSDMEVQASVPMQQAMAGSIEPKDAVKQIRAAMQELVDTPKPF"
     gene            complement(2679..>3653)
                     /locus_tag="VHM69_00410"
     CDS             complement(2679..>3653)
                     /locus_tag="VHM69_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX2738877.1"
                     /translation="PNLYDLSLELLDEGGEVLDAVESYFGMRKIETKDGKVYLNGRPY
                     YQRLILDQGYFPEGILSAPTDDDLRGDIELAKEMGFNGARKHQKVEDPRWLFWADSLG
                     FLVWGEMANAYQYSQDYVQRITAEWQEAVMRDYNHPCIVAWVPMNESWGVPNLAGRRD
                     QAEHLLSLYHLTRSLDLTRPVVSNDGWEHALTDLCTIHDYRDAEALAKSYATSESAVA
                     AEPANRPIYVPGYGYRGEPILITEFGGIAFSGDETGWGYSTVANSEEFLERYESLIDA
                     LLHSEPVQGFCYTQLTDVEQEVNGLLTYDRNPKADLTRIRGITSRPKP"
BASE COUNT          674 a         1120 c         1145 g          714 t
ORIGIN      
        1 ggtcagggcg ctgttgaagt accagcgaag caggttcccg gcgctgagca cctcggcgaa
       61 ggactcgaag gtgaaccctg acggggggat ccagctgaca ggaatggccg tggtgtccgc
      121 ctcggtcttg agcgccgtgt cgacggccca cgccagcgga ataagccaga ttatcgctgc
      181 cacgagcaat actgcgaagg cgagccagtt gccaagcctg cggcgtccgg taccggctgt
      241 cttctcctgg atctctcccg gagttcttgc ctctgtcacg gtcatcaggc ttccctcctc
      301 ctgcggcgtg ccaacacgaa ctgcgtgatg ttgaagacga gtatgagcac gaagaacacg
      361 taggacatag cggcggcgaa gccgacccgg tactgggtga agccctgctc gtagacgtac
      421 tggatgatcg ggcgcgtcac cgtgtagttg agccccccgg acgtgttcat gatgtagatc
      481 tggtcgaaca cgaacagcga tgcgagtatc tggagcacca cgatcaggaa ggtggtgcgt
      541 gatagaagcg ggatcgtgat gtagcgagtc tgcgcaaagc cgcccgcccc gtcgagggcc
      601 gcggcctcgt agagctcctc ggggatctcc tgcagcccgg ccagatacag cacgaagttg
      661 aacccgatag tccaccacac ggtggcgatg acgacggaga tcatggcgac gttctcaccc
      721 gcgagccagt cgacctccgg caacccgagc gagacgaggt agctgttgat caggccgaaa
      781 ccgggctggt acagccatac ccagatgagc gccacaacgg acggggccag cacgtaaggc
      841 gcaaagaagg cgacccggaa gaaccaccgc gccggtacgg cccggttagc cagaagcgcg
      901 agccctagag cgagcagcac tagcggtggg gtgctcagca aggtgaacag tatcgtgtgc
      961 cacagcgagg cccagaagtc ggggtcgctg aatagctccc tgaagttggc gagccccagg
     1021 aagtctctcg tacccgtccc gaccaggctc cagtcgaaga agctcatccg caggcccagg
     1081 ataatgggcc acacgaggaa gagtgcgtag gcgatgagga aaggcaggac gaagaggtat
     1141 cccgtgagat cgatcctcct ctcccgttcc cctttggccc tggtctgctg cgcggtctcg
     1201 gcccgtgccg ccaatcctgt acctccccgt cctttggtct tccgttagaa gggcttgggc
     1261 gtgtcgacga gctcctgcat cgcagcccgt atctgcttca ctgcatcctt tggctcgata
     1321 ctgcccgcca ttgcctgttg catcggcacc gacgcctgaa cctccatgtc cgagccggag
     1381 ccgctgaacc aggccttggg atcgaggacc accttgtcgg cgaccccggc gtagttgctc
     1441 tgcggcttga ggttcttgta ctcggagctc tccagcacgg accggtaagc cgggatgtgc
     1501 ccgccctcgg cccagatcag gctgctcttt agcatggaac tgatgaactt gagcgcgccg
     1561 tcgaacgcct ttgagtcgtt ggcgatgccc ttgggtatga cgaaggtgtg gcggtcggcc
     1621 tggacgtgct tgttgccgaa cacgtcgggg aactcggtca tgctgaaggg catcttggca
     1681 tcgaggaagg tcgtgacctc ccactcgccg ttccagtgga acccggcgtt gccgctctgg
     1741 aactgcgcca ccgcggcgcc gtagtcctgg ttgcttggcg taaactcttt gtccagcgtg
     1801 agctcgacca ggaaagatag ggcctcctcg gccttctcct gatccaggat cacctcgctc
     1861 gcgtcgggtg agagcacctc tcccccgagc tgcccgtaga agctgtagaa gagccgccac
     1921 ggaactaccg ggtcagcggg cgcgaacgcg atgccctgct ccttggtgac ctccatagac
     1981 ttctggaacg cgtcgatgag ctcgtctcgt ccctgaagtg gtttgaggtt gccgtcggaa
     2041 tccagcagcc cggcctcttt gcagatgtcg gtgttgtaga acatgacctg cgggtgggta
     2101 tccaggggga tggcgtagat ctctccgttg tacttgccgc cttcccatat ctcgggcaag
     2161 aacttgtcct gaccgatgtc gtactccgcg agcacgtcag gagccagagg ctccagcaac
     2221 ccagcaggcg cgtacgccgg tagcctggtc aggtgcataa ccgcaacgtc cggtggccgc
     2281 ccaccgaccg tggccatcga gaccttcgtg tagtacggag cgccccacgt tagcgttgtc
     2341 gccgagagat cgatttcagg gtgggactgt atgaagtcgc tctccatgtc ggcaagccgc
     2401 gcgccatcgc cgccaccaaa gaggttccag aaatggacct tctgcttcga actgccaaat
     2461 ggtccccccg aacaggaagc caacgccatc ccgccgagag ccgtcgcagc accgtacaag
     2521 aaccgccgcc tgctgacccg gcgcacggcc tttgcaccgg tcccgatctg ccctcggtcc
     2581 atgcccttcc cctttcaggc gaacgctcgc cttacctgtg ccgcatgtta tcatccgcta
     2641 ccggggtggt caacagattt tcgggattct ggcccgggtt aaggcttcgg gcgtgaagtt
     2701 atccccctta tgcgggtgag atccgccttc ggatttctat cgtaggtaag tagaccattg
     2761 acctcctgct ccacgtcggt gagttgcgtg tagcagaagc cctgaactgg ctcgctgtgc
     2821 aacagagcat cgatgaggga ctcgtagcgc tcgaggaact cttccgagtt ggcaactgtg
     2881 ctatagcccc agcccgtctc atcgccggag aaagcgatac cgccgaactc ggtgatcagg
     2941 attggttcgc ccctgtagcc gtatcctgga acgtagatgg ggcgatttgc gggctcggcg
     3001 gcgacggcgg actcactcgt ggcgtagctc ttcgcgaggg cttcggcgtc gcggtagtcg
     3061 tggatcgtgc agaggtcggt cagggcgtgc tcccagccgt cgttggagac gacgggccgg
     3121 gtcaggtcga gagagcgcgt caggtggtac agggagagta gatgctcggc ctgatcccgc
     3181 cgtcccgcca ggttggggac gccccagctc tcgttcatcg ggacccaggc gacgatgcag
     3241 gggtggttgt agtcacgcat gacagcctcc tgccactcgg ctgttatgcg ctgcacataa
     3301 tcctggctgt actggtatgc gttcgccatc tcgccccaga ccaggaatcc gagggagtcg
     3361 gcccagaaca gccagcgcgg gtcctcgact ttctggtgct tgcgggcgcc gttgaaaccc
     3421 atctccttcg ccagctctat gtcaccgcgc aggtcgtcgt cggtaggggc tgaaaggatg
     3481 ccttcgggga agtagccctg gtcgagtatg agacgctggt agtaaggacg tccgttcagg
     3541 tagaccttgc cgtctttggt ctcgatcttc cgcatcccga agtagctctc gacggcatcg
     3601 agaacttctc ccccttcatc gagcaactcc aggctgaggt cgtagaggtt ggg
//
LOCUS       DATAIR010000017         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_699, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000017 DATAIR010000000
VERSION     DATAIR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VHM69_00415"
     CDS             complement(<1..425)
                     /locus_tag="VHM69_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2738878.1"
                     /translation="MSLAIETERLTKTYGRKARGIEDVDLAVEEGEVFGFLGPNGAGK
                     TTTVRTLLGFLRPTGGRGEVFGLDIKKDSVEIRARVGNLPGEFSLEDRMTGEGLLKYF
                     ARLRGVEDLGYARELSERLGAELHRPMRRLSRGNKQKIGL"
     gene            complement(459..956)
                     /locus_tag="VHM69_00420"
     CDS             complement(459..956)
                     /locus_tag="VHM69_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2738879.1"
                     /translation="MEEERREYIEEFGLLFGQFGLSRMFGRVLGVLMISDPPERSAEE
                     LAEELGASRGSISQTTRSLIQMGLVQRRSKPGERRDYFRIKPGAWHEIMRREMDALGG
                     FRKTAERGLELLDSNDPSARRSLEEMRDFYTYWEKEMPAVLQRWEKESRRRGPEPLDS
                     IIRQS"
     gene            complement(1086..1766)
                     /locus_tag="VHM69_00425"
     CDS             complement(1086..1766)
                     /locus_tag="VHM69_00425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2738880.1"
                     /translation="MARSGLFELWATVLAAMLAAILLSATSFMSDAGAQEPPCTGQVV
                     LDPGHGGTDSGAVNTKYKLTEKAQTLDVALKLKTLLESDGYTVCMTRTTDKSLSNNDR
                     YTYANTTGAGVLVSIHMNGSSNSSHDYTTTLFGKWRKDKELATTVFESLSALPAAGAT
                     GTIATKQPYSFASGVLLKSEMPATIAETVFITSDREGQLLSDGTGTRQQQIAEALKVG
                     VENYLTTH"
     gene            complement(1884..2504)
                     /locus_tag="VHM69_00430"
     CDS             complement(1884..2504)
                     /locus_tag="VHM69_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738881.1"
                     /translation="MDRGEERIGLNGAAAVLDVPPETVRRFVQEGRLRSEREEDGGFS
                     FYLKDVMGLARELGAESTEEEIFEGEVMGDEPSGNSRLPAVPFERYERLLQQGQDYKT
                     RLEERTRQIEEVRQERDAARMEIDRLWSEIERLNLVEFQRELQEKTISTLEEEKARLE
                     SERERLTKEQMALTEEKSALSEDRVRLEAELNSLKNRGFWSRLFGG"
     gene            complement(2670..3170)
                     /locus_tag="VHM69_00435"
     CDS             complement(2670..3170)
                     /locus_tag="VHM69_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2738882.1"
                     /translation="MEKISRERFIRLGTALGVGAATASLAACGGGGNTGGGSGGGNGG
                     GGGSGGDYGGQNSGGSSQAADAKAGGAAIASESEVAPGSAVTFKDAGQDAILVHLKSG
                     DFVAYSATCTHQQCTVAYKGGKIACPCHGSVFDPARGAEVVAGPAPSPLPEIPVKVVG
                     GEVVRA"
     gene            3405..>3629
                     /locus_tag="VHM69_00440"
     CDS             3405..>3629
                     /locus_tag="VHM69_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738883.1"
                     /translation="MNGMVWVRVLIGAVWLNGGLEKLLNPDFPRQFAASLQAGGFVSQ
                     APPSFQDFMNGYVIPNAELFAQLMRAGELTL"
BASE COUNT          630 a         1169 c         1033 g          797 t
ORIGIN      
        1 agcccgatct tctgcttgtt cccccgcgag agcctcctca tgggacggtg caattcagcc
       61 ccgagcctct cggatagctc gcgcgcatac cccagatcct cgacgccacg taacctggcg
      121 aagtacttga gaagaccctc ccccgtcatc ctgtcctcca gggagaactc gccaggcaag
      181 tttccaaccc tggcccttat ctcgacactg tccttcttta tgtccaggcc gaagacctcg
      241 ccacgtccgc ccgtcggccg taggaacccg agcagcgtcc ttacggtcgt tgtcttgccc
      301 gcaccgttcg gcccgaggaa cccgaagacc tcgccttcct ccaccgcgag atccacatcc
      361 tctatgcccc gcgctttgcg gccgtaggtc ttggttagcc gctctgtctc tatcgcaagg
      421 ctcatcacca ctccttttta tttaacctaa tataaagttt agctttgtct aattatactg
      481 tcaagaggtt ctgggcctct tcttcgcgac tctttctccc accgttgaag gacggctggc
      541 atctcttttt cccagtaggt atagaagtcg cgcatctctt ccaggctacg tctggcgctg
      601 ggatcattgg agtccagaag ctcgagtcca cgctctgccg tcttgcggaa accgcccagc
      661 gcgtccattt cgcggcgcat gatctcgtgc cacgcccccg gctttatcct gaagtagtcc
      721 cgcctctctc ctggtttgga ccgccgctgg acgagaccca tctggatgag cgaccgcgtc
      781 gtctggctga tggagccccg gctcgctccc aactcctcgg ccaactcctc agccgaccgc
      841 tccggcggat cggagatcat caataccccc aatacccgcc cgaacatccg cgagagcccg
      901 aactgcccga agagaagccc gaattcctcg atgtactccc tccgctcttc ttccaatcta
      961 cctcctcaat gcttcgccac agaaaccagt atattcccat gtttcacata tttcaatgtt
     1021 tgctgaaagt tatgaaagta aggtaggctg gatggggtac gctggtcttc ggtacaccgg
     1081 gtaggttagt gggtcgtcag gtagttctcg acgcccactt tgagggcttc ggctatctgc
     1141 tgctggcgcg tcccggtacc gtcggagagg agctgtccct ctctatcgct ggtgataaag
     1201 acagtctccg cgatggtcgc cggcatctcg ctcttgagga ggacgcctga ggcgaaggaa
     1261 tatggctgct ttgtggcgat ggttccggta gcgcctgccg cgggaagcgc agagaggctc
     1321 tcgaacactg tggtggccag ttccttatcc ttgcgccact ttccgaagag ggttgtcgtg
     1381 taatcgtgag aggagttcga ggagccgttc atgtgtatgg agacgaggac accagcccct
     1441 gtggtgttgg cgtatgtata gcggtcgtta ttcgagaggg acttgtctgt cgtgcgagtc
     1501 atgcatactg tgtaaccgtc gctctcgagg agggttttga gttttagtgc gacgtcgagg
     1561 gtctgggctt tctcggtaag cttgtatttt gtgtttaccg cccctgagtc agttcctccg
     1621 tggcctggat ccaggacaac ctgccccgtg catggtggct cctgcgcccc tgcgtcgctc
     1681 atgaaggaag ttgcggagag aaggatcgcg gcgagcatcg ctgccagcac cgtcgcccac
     1741 agctcaaaga ggcccgatcg cgccatcccc catcctttcg aaatggggcg gagggaagga
     1801 gcgggctccg ccccgcgtta cgtaacggca ggtagcatac ccgggtaagg atgctgtgac
     1861 atcccccaaa tgggctattt ttgtcagccg ccgaagagtc gggaccagaa gccgcggttt
     1921 ttcagggagt tcagttcagc ttcgaggcgg acgcggtctt cagagagggc tgacttctcc
     1981 tccgtgaggg ccatctgttc tttcgtcagt cgctcgcgct ccgattccag cctggccttc
     2041 tcctcttcga gggtggagat cgtcttctcc tgcagttcgc gctggaactc gaccaggttg
     2101 aggcgttcga tctcggacca gaggcggtcg atctccatcc tcgcggcgtc gcgctcctgg
     2161 cggacttcct cgatctggcg cgtccgctct tcgagacggg tcttgtaatc ctggccctgt
     2221 tgcaggagcc gctcgtagcg ctcgaaaggg acggcgggga gccgggagtt acccgagggc
     2281 tcgtcgccca tcacctcacc ctcgaagatc tcctcctccg tggactccgc gccgagttcg
     2341 cgcgccaggc ccataacatc cttcaggtag aaggagaaac caccgtcctc ctcgcgttcg
     2401 gagcggagcc ttccctcttg cacgaagcgc cggaccgtct caggcgggac gtccagaacg
     2461 gccgccgccc cgttaagccc gatgcgctcc tctcccctgt ccatgagcgc attctacttg
     2521 agattaaggc atcaggcatc aggttacagg ctgcaggttc gccctgtaca cacagcgtaa
     2581 agatcccgat ccagcctcag ttgaagatgg gccgggacgc tcggtcgatt cctttcctga
     2641 ggcctgaaac ctgtaacctg aaaactattt caggcacgga cgacctcgcc gcccacgacc
     2701 ttcacgggga tctccggcag cgggctcgga gccggacctg ctactacttc cgcgcccctg
     2761 gcggggtcga agactgagcc gtggcatgga caggctatct tgccgccttt gtaggctacc
     2821 gtgcattgct ggtgggtgca ggtagcagag taggccacga agtccccgct cttcaggtgg
     2881 acgaggatgg cgtcctggcc cgcatccttg aacgtgacag cgcttccagg cgcgacctcg
     2941 gactcgctcg cgatagccgc tccacccgcc ttcgcatctg ccgcctgcga acttcccccc
     3001 gagttctggc caccgtagtc accacccgag cctccaccgc cgccattccc gccacctgaa
     3061 cctccgccgg tgttgccgcc tcccccgcag gcggccagtg aggcggtggc ggcgcccact
     3121 cccagggcgg tgccgagccg gatgaacctc tcgcgcgata tcttctccaa ggcttcctcc
     3181 tcgtcgctag gggtacccat gatatacgca tggtctcaga ctccggttct accatggggc
     3241 gtccgaaata gagcagtcgt agagaagagc cgagcccagg ttcttccagg acaagcaccc
     3301 ttcgatccgc atttgtatgc gtgggcgttc tgtgcggcat cccgcaggtc cacgggtgcg
     3361 tatatgattg atagagtcgc caacccgtga gaggagaccg atctatgaac ggcatggtgt
     3421 gggtgcgcgt cctgatcggg gccgtatggc tcaacggcgg cctggagaag ctgctgaacc
     3481 cggattttcc acggcagttc gcagcgagcc tgcaggccgg gggcttcgtc tctcaggccc
     3541 cgccgtcctt ccaggacttc atgaatggat acgtgatccc caacgccgag ctcttcgccc
     3601 agctcatgcg cgcgggagaa ctgacgctt
//
LOCUS       DATAIR010000018         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_323526, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000018 DATAIR010000000
VERSION     DATAIR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323526"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHM69_00445"
     CDS             <1..264
                     /locus_tag="VHM69_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX2738884.1"
                     /translation="ISLLTGVKVSDCTNGYRAIRVSELHKLNLREDRFNANEIILEAL
                     KHKLRFGQVPVSMMSRAAGETKKPPKLAYPLGVFRVIISTWLR"
     gene            401..1657
                     /locus_tag="VHM69_00450"
     CDS             401..1657
                     /locus_tag="VHM69_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738885.1"
                     /translation="MPDLSKRQQSAGAGSVYIAGSFAVAGVLTYAFQSLSIHALGKEG
                     YAPLALLWSATFLTVQVLWIGATQTLGRYVAEREAHSRDWGPVVSSVRRWQIGLVVAF
                     VLVALLASPLLTGELFQDPWLTAAFIVAVVLYAPEYFRRGIFNGHRQSFRLGAQIVAE
                     SASRVLVAAVLLAVGWGVFGPAIAIVLAPLIGVLAVRPAPVSAPEREGEPFSTWGAFR
                     FAGPVLACMAFAQVLMNGGPILARLLGGTDAQVSVLAAAVILTRIPQYVLSPAIGALL
                     PRASRVLSTEGRISFDRFVVRAVGVVGIVGVLMVVGTWLFGGWGMRLFASFGAEREVL
                     VALAAMAAFYLLSETLNQGLFALGHGRLAALGWFVGLLASAACMAVIGAGIVERVSYA
                     LAAGTFFAAVAQAVFYLVTRRAAAGN"
     gene            complement(1659..2639)
                     /locus_tag="VHM69_00455"
     CDS             complement(1659..2639)
                     /locus_tag="VHM69_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2738886.1"
                     /translation="MNWLVTGGCGFIGTALIRSLMAESGHGVRVVDNLAVGTREDLAA
                     ASDFVEVSSGDPGPMDPERVELIVGDITDDDLALRAAQGADVIVHFAANTGVMPSVED
                     PRADCMSNVVGTLNYLEAARHAGVGRFVFASSGGTVIGEAEPPIHEEMVPHPVAPYGA
                     SKLAGEGYCSAYFQTFGIETVALRFGNVYGPLSGHKNSVVARFIKRATGGEVLEIYGD
                     GTQTRDFIYVDDLIRAVKLSAIVDGVGGEVFQIATSAETTVQELTDRLLPALAASGIK
                     DIEVRKTAARKGEVRRNYADTSKAERMLGWKAEVGLDEGLRRTVDFFVET"
     gene            2929..>3368
                     /locus_tag="VHM69_00460"
     CDS             2929..>3368
                     /locus_tag="VHM69_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2738887.1"
                     /translation="MTRRTLTRRDFLKVAGAGAAGATMLGAAGCSTLTSNLTQVPDEY
                     LPSGGSRMNVILVIIDSLRRDHLGVYGNDWIKTPNLDALARESLRFDRSYPESLPTIL
                     ARRAIHTGIRTFPFRNWQKWYEEDVNLWGWQPIPRGQVTLAEILL"
BASE COUNT          556 a         1098 c         1074 g          640 t
ORIGIN      
        1 atctcgctgc tcaccggcgt gaaggtctcc gattgcacga acggctaccg cgcgataagg
       61 gtctccgagc tccacaagct gaacctgcgc gaggaccgct tcaacgccaa cgagatcatc
      121 ctcgaagccc tcaagcacaa gttgcgtttc gggcaggtcc ctgtctcgat gatgagccgc
      181 gccgccggcg agaccaagaa gcccccgaag cttgcatacc ctctcggggt cttcagggtc
      241 ataatctcga cctggctgag atagaggctt caggtttcag ccgtcaggca agcatagcag
      301 gacggcctct ctttcagcga gaacgtctcc ccgcccgtgg tgcgcgaaaa cgaaatacac
      361 tttcgttcat gagagtagaa tacctgatac ctgaagcctg atgcctgatc tatccaaacg
      421 gcaacagagt gccggtgccg gaagcgtcta catcgcgggg tccttcgccg tcgccggggt
      481 gcttacctac gctttccaga gcctttcgat ccacgccctc ggaaaggagg gctacgcccc
      541 gctcgctctg ctgtggtcgg ccactttcct gaccgtgcag gtgctctgga tcggcgcgac
      601 ccagaccctg ggccgctacg tcgcggagcg tgaggcgcac agtagggact ggggtcccgt
      661 ggtctcatcc gtgcggcgct ggcagatcgg gctggtggtg gcgttcgtgc tcgtggcgct
      721 gctggcgagc ccactgctca caggggagtt gttccaggac ccgtggctca ctgccgcgtt
      781 catcgtcgcc gtcgtgctct atgcgcccga gtacttccgg cgcggcatct tcaacgggca
      841 caggcaatcg ttccggctcg gggcccagat cgtggccgag tcggcgagcc gggtgctggt
      901 cgccgcagtt ctgctcgccg tgggatgggg cgtcttcggg ccagccatcg ccatcgtgct
      961 cgccccgctc atcggcgtac ttgcggtcag gccagcccca gtgtcggcgc ccgagaggga
     1021 gggggagcct ttctccacct ggggggcttt ccggttcgcc ggaccagtcc tggcgtgcat
     1081 ggccttcgcc caggtactga tgaacggagg cccgatactg gcgaggctcc tcggcggcac
     1141 ggacgcccag gtgtcggtgc tcgccgccgc agtaatactc acgcgtatcc ctcaatatgt
     1201 cttgagtccc gccataggcg cgctcctgcc ccgcgcgagc cgcgtgctgt ccaccgaggg
     1261 acgcatctcc ttcgaccgct tcgtggtaag ggcggtcggg gtcgtcggca tcgtgggtgt
     1321 cctcatggta gtgggaacct ggctcttcgg gggatggggg atgaggctgt tcgcgagttt
     1381 cggggctgaa agggaggtgc tcgtggcgct ggccgcgatg gccgccttct acctcctctc
     1441 agagacgctc aaccaggggc ttttcgccct cgggcacggt cggctcgccg cgctagggtg
     1501 gttcgtcggg ctactcgcct ccgccgcgtg catggcggtg atcggggcgg ggatcgtcga
     1561 gagggtctcc tatgctctgg ccgcgggcac cttcttcgca gccgtggcgc aggccgtgtt
     1621 ctacctggtg acgcgcagag cagccgccgg aaactagttt acgtttccac gaagaagtcc
     1681 acggttcgtc tgaggccctc gtcgaggccg acctcggcct tccagcccag catcctctcc
     1741 gccttcgagg tgtcggcgta gttgcggcga acttcgcctt tgcgggccgc ggtcttgcgg
     1801 acttcgatgt ccttgatgcc tgaggcggcg agggctggga ggagtctgtc cgtcaactcc
     1861 tgcaccgtcg tctcggcgct tgtggcgatc tggaagacct cgccgccaac cccatcgacg
     1921 atggccgaga gcttgacggc acggatcagg tcgtccacgt agatgaaatc gcgtgtctgg
     1981 gtgccgtcgc cgtagatctc cagcacctct ccgcctgtcg cccgcttgat gaagcgcgcg
     2041 acgacgctgt tcttgtggcc ggagagcggc ccgtatacgt tgccgaaccg caacgctacg
     2101 gtctcgatac cgaaggtctg gaagtacgcc gaacagtagc cctcgcccgc gagcttgctc
     2161 gccccgtagg gcgcgacggg gtgcgggacc atctcctcgt gtatcggcgg ctcggcctcc
     2221 ccgataaccg ttcccccgct ggaggcgaag acgaaacgtc ccacaccagc gtgcctggcg
     2281 gcctccaggt agttgagggt gccaacgacg ttgctcatgc agtccgccct cgggtcctcg
     2341 accgaaggca tgacccccgt gttggccgcg aagtggacga tcacgtcagc accctgcgcc
     2401 gcgcgcaagg cgagatcgtc gtcagttatg tcccctacta tcagctcgac tctctcggga
     2461 tccatcgggc cggggtcccc tgaggacact tcgacgaagt cgctggccgc cgccaggtcc
     2521 tcgcgcgttc ccacggccag gttgtccacc acccttacgc cgtgaccgct ttccgccatt
     2581 agagaccgga tcaaggcagt cccgataaac ccgcatccac ccgtaacgag ccagttcacg
     2641 aggacagcgc ctgggagaga gtgccttccg tgtttttcat acgtgggtgc atatccatcc
     2701 ttggtagacg ttttacgccg cgcatacctt agactaacct ccggtccggt gcgagtcctg
     2761 cggacggagg cgctattcgc tacaatctgc ggcggctgat tatatgcaac agggaaggcc
     2821 attgtagatg cacacgaaga cgctcctcgc tctagagaag ttcgacgagc gcgccggtct
     2881 ggagaagacc gatgccctcg cttgccgcct tgtacccgga ggcgaggggt gacgcggcgc
     2941 accctcacgc gcagggactt cctcaaggtg gccggtgcgg gggccgcggg ggcgaccatg
     3001 ctcggggcgg caggatgcag caccctcacc agcaatctca cacaagtacc agacgagtac
     3061 cttccgagcg ggggatctag gatgaacgta atactggtga tcatagacag cctcagaaga
     3121 gaccacctcg gggtctacgg caacgactgg atcaagacgc ccaacctcga cgccctcgcc
     3181 agggagagcc tgcgcttcga ccggtcgtat cccgagtcgt tgcccaccat cctcgcgcgg
     3241 cgcgcaatcc acacggggat aaggactttc cccttcagga actggcagaa gtggtacgag
     3301 gaggacgtga acctctgggg ctggcagccc atcccgaggg gccaggtcac gctcgccgag
     3361 atcctcct
//
LOCUS       DATAIR010000019         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_278040, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000019 DATAIR010000000
VERSION     DATAIR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278040"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VHM69_00465"
     CDS             <1..297
                     /locus_tag="VHM69_00465"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEX2738888.1"
                     /translation="GVAIAAAALFLSHGVSFVVNFLGEREYLNVSPDKQMMEPYSRVV
                     VLHATILGGGLLAAYLGTPLASLVLMIVLKTALDLRAHLREHRTARQRPATTAS"
     gene            complement(300..1475)
                     /locus_tag="VHM69_00470"
     CDS             complement(300..1475)
                     /locus_tag="VHM69_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2738889.1"
                     /translation="MDVSQRFEGLQAEVEERFGEKIVALRRDIHREPELGFETEKTAE
                     KVLAALEGLPLEIETGIAQNGIVATLRGHEGPTVALRADMDALPIEEDTGLPFASEID
                     GKMHACGHDAHTSMLVGAVHALSGMRDRLNGTVRFVFQPAEEGGGGGKVMVEEGVADD
                     VSSIFALHLWPGLPFGKVATKAGPIMAAADAFEMEIKGSGGHGAMPHLAADAVVIAAH
                     VVTALQTVVSREVDPVEPAVLTVGEIGAGTAFNIIPEKVRIGGTVRTLNAALRERMPG
                     RIEEIARGVAKGMRGDADLDYHFSYPVTVNDEGAAGRALGVAEELFGGESVLELPNPS
                     MGAEDFAYFLQKVPGAFIWLGVGEDVSGLHTPQFAFDEEVLPRGSALLAALALESLT"
     gene            complement(1542..2492)
                     /gene="gluQRS"
                     /locus_tag="VHM69_00475"
     CDS             complement(1542..2492)
                     /gene="gluQRS"
                     /locus_tag="VHM69_00475"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX2738890.1"
                     /translation="MRDSARHSREEIGVSGYDGRFAPSPTGPLHLGNLRTALLSWLFA
                     RSAGARFLVRMEDLDRSRVRPGIEEEQLADLRAIGLEWDGPVVRQSERMALYKEAIAR
                     LDDRDLLYPCYCTRAEIRAAASAPHGISTADRYPGTCRELTRAERAEREAAGRPPALR
                     VRSEGARIMFEDRLLGRFEEEVDDFVVRRNDGTPAYQLAVVVDDAAQGIGEVVRGADL
                     ADSTPRQILLARMLGLPEPRYAHVPLVLGPDGQRLAKRHGAVTLADREEGPQEVRAWM
                     ARSLGLAEAGETPTPDELLARFDPDRLAREPTVWSPDAAV"
BASE COUNT          470 a          939 c          815 g          495 t
ORIGIN      
        1 ggggtggcga tcgccgccgc cgctctcttc ctgagccacg gcgtctcgtt cgtcgtcaac
       61 ttccttggtg agcgagagta cctcaacgtc tcccccgaca aacagatgat ggaaccctat
      121 tcgcgcgtcg tcgtcctgca cgcgacgatc ctcggcggag gattactcgc cgcctacctg
      181 gggacgccgc tggcctcgct cgtgctcatg atcgtactca agacggccct ggatctccgc
      241 gcccacctga gggagcacag gacagcgcgg caaagacctg ccacgacagc ctcttagctc
      301 tacgtgagcg actccagcgc gagcgcggcg agaagggccg acccgcgcgg caaaacctcc
      361 tcgtcgaagg cgaactgggg cgtgtgcaac cccgagacgt cctccccgac gccgagccag
      421 atgaaagcac caggcacctt ctgaagaaag tatgcaaagt cctctgcccc catcgaaggg
      481 ttcgggagct ccaggacgga ctctccgccg aagagttcct cggcgacgcc gagggcgcgt
      541 cccgccgcac cttcgtcgtt caccgtcacg ggataggaaa aatgatagtc gaggtccgcg
      601 tcgcctcgca tccccttcgc gacgccacgg gcgatctcct ctatgcgccc cggcattctc
      661 tctctgagag cggcgttgag tgtacggacc gttcctccaa tgcggacctt ctcagggatg
      721 atgttgaatg ccgttcctgc gccgatctcc ccgaccgtca ggaccgcggg ctcgacgggg
      781 tcgacctccc tcgagacgac ggtttgcagt gccgtcacga cgtgcgcggc gatgacgacg
      841 gcgtcggcgg ccaggtgggg catcgcgccg tgaccgcctg aacctttgat ctccatctcg
      901 aacgcgtctg cggcggccat gatcggaccg gccttcgtcg ccactttccc gaacggcaac
      961 ccgggccaca ggtgcagcgc gaagatggag gatacgtcgt cagccacccc ctcctcgacc
     1021 atgactttgc ctcctccccc accttcctcc gccggctgga agacgaacct gacggtgccg
     1081 ttcaacctgt cgcgcatccc cgagagggcg tggaccgcgc cgacgagcat gctggtatgc
     1141 gcgtcgtggc cgcatgcgtg catcttgcca tctatctcag aggcgaaagg caggcccgta
     1201 tcctcttcta tagggagggc gtccatgtcg gcccgcagcg caaccgtggg tccttcgtgt
     1261 cctcggagcg tggcgacgat accgttctgc gcgatgcccg tctctatctc gagaggcaga
     1321 ccttcgagag ccgcgagcac cttttctgcg gtcttctccg tctcgaatcc gagctcgggc
     1381 tcacggtgaa tgtcacggcg aagagcgacg atcttctctc caaatcgctc ttcgacctca
     1441 gcctgcaacc cttcaaacct ctgagacacg tccacgaagt ctcctctcat cagaacctct
     1501 catgcgccgc agcgcacagg aaagttacca tagagcgagg ctcagaccgc agcatcgggg
     1561 gaccagacgg tgggctctcg cgccagacgg tccgggtcga agcgggcgag cagctcgtct
     1621 ggcgtcggcg tctctcctgc ttcggcgagg ccgagcgagc gcgccatcca tgctcgcacc
     1681 tcttgcggcc cctcctctct gtccgcgagg gttacggcgc cgtgacgctt ggcgaggcgc
     1741 tgtccatccg ggccgagcac tagcgggacg tgggcgtatc gtggctcggg caggccgagc
     1801 atgcgggcga gaaggatctg acgcggtgtc gagtccgcga ggtcagcgcc cctcaccacc
     1861 tcgcctatgc cctgcgcggc gtcgtccacc acaacggcga gctggtacgc tggcgttccg
     1921 tcgttgcggc gcacgacgaa gtcgtccacc tcttcctcga agcgcccgag caaccggtcc
     1981 tcgaacatta tgcgagcgcc ctcagatcgc acccgcagcg ccggcgggcg tccggcagcc
     2041 tctcgttcgg cgcgttctgc tctggtcagt tcgcggcatg taccgggata gcggtctgct
     2101 gtggagatgc cgtgcggggc ggatgctgcg gcccggatct ccgcccgcgt acagtagcag
     2161 gggtagagaa gatccctgtc gtcgaggcga gcgatagcct ccttgtacag cgccatcctc
     2221 tcagactgcc tgactacagg tccgtcccat tcgagcccga tggcgcgcag gtcggcgagt
     2281 tgctcttcct cgattcctgg ccgaactcgc gagcggtcaa ggtcttccat gcgcaccaga
     2341 aagcgtgccc ctgccgaacg cgcgaacagc catgacagga gcgccgtccg caggtttccg
     2401 aggtggagcg ggccagtggg gctcggtgcg aagcgtccgt cgtaaccgga gacaccaatc
     2461 tcttctctgc tgtgtcgtgc agaatcccgc aaagcgagcc agagtctaca acctctgtgt
     2521 ttctcagtac gctttccggt tgaacagcca cagggtggcc aggagcggca atgctgctgc
     2581 gatcaggcac acgaaaaatt agtgaatgcg aatcccaccc gagccagcac ctccaggctc
     2641 acgtactctg acaccgaaat ctcaccagtc ccacgcgtat ccaccatcat tcctgcacct
     2701 ctcccctgtc atctctcgg
//
LOCUS       DATAIR010000020         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_1046, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000020 DATAIR010000000
VERSION     DATAIR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..326
                     /locus_tag="VHM69_00480"
     CDS             180..326
                     /locus_tag="VHM69_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738891.1"
                     /translation="MGRRHRSEVCYGPPDVLELRDIDKPEIEDEEVLVRVHAAGVDRG
                     YGTS"
     gene            323..487
                     /locus_tag="VHM69_00485"
     CDS             323..487
                     /locus_tag="VHM69_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738892.1"
                     /translation="MTGLPYAKRLAGYGLRAPTNPVLGMDVAGVVEAVGIDYTQEDFA
                     EGDQRYDLMP"
     gene            870..1190
                     /locus_tag="VHM69_00490"
     CDS             870..1190
                     /locus_tag="VHM69_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738893.1"
                     /translation="MYARISTLAGSPDQIDEGLRYVRDNVLPQLQQQDGFKGMTTLAD
                     RESGKTPGITFWEGEEALRASEEAAEKMREDSAEAMADTIASVERFEVGFMAMSSYSR
                     SPIG"
     gene            1498..1803
                     /locus_tag="VHM69_00495"
     CDS             1498..1803
                     /locus_tag="VHM69_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738894.1"
                     /translation="MKFRENRAAGWGVREFHNVEPPPQGPRPGHIDLGWSLKVVQRGT
                     KQTTGRLWDVTVYYQVGRKKRVYHGAEANRILQTVQENRSDRQQPHPAAVSQVSQAE"
     gene            2146..3177
                     /locus_tag="VHM69_00500"
     CDS             2146..3177
                     /locus_tag="VHM69_00500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2738895.1"
                     /translation="MLTSYGLMENLLSSLALTVFVGILALLAQWSRKSRRAEISLLVV
                     VVFASLLLLVTGALLGVLWISGQMPSGVYPRKLLAVTCVPVALAGLIGLALCVPPLRK
                     ILGNRPNGEFWTDPPIFLALWLFVTVLLANNLVGILGFEQLEQGGAFALGNGGRIPPV
                     AILASQLPFVVLALLGVGAGIRRTPRETLARLGYGPISLKHLGIVMLFIAGAFALSLA
                     ADYLFSQAQPELYRKVGEVSGTLFNPKGLSLVSAILFALLIGVGASLGEETLFRGAVQ
                     PVFGIAATSVLFASMHVQYGPSLLLGYIFLLSVGLGILRRHINTTASFLAHASYNTLG
                     ILAAYFFGV"
     gene            complement(3186..3689)
                     /locus_tag="VHM69_00505"
     CDS             complement(3186..3689)
                     /locus_tag="VHM69_00505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2738896.1"
                     /translation="MRFSLKPITPDDAKSISRWRYGGRYAVYDGDPTSVASLLEPRLL
                     YHSVYDEGGDLVGYFCFGEDARVSAGRDIYRREAALDVGLGMRPDLAGRGLGEEFVRA
                     GLKYAKEAYSPPAFRLTVASFNHRAIRVYERAGFQKVETFGAPTQDGEPEWVLMRREA
                     GADPPRA"
     gene            complement(3708..4007)
                     /locus_tag="VHM69_00510"
     CDS             complement(3708..4007)
                     /locus_tag="VHM69_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2738897.1"
                     /translation="MKTLEISPERHRGDLLSTIGETLRAERNERGLTLKQVAEGAHVS
                     VSYLAEIERGEKDPSSRVLESIARGLDVEVSELLVRIASALEPAPVVPVTNSLAA"
     gene            complement(4018..4908)
                     /locus_tag="VHM69_00515"
     CDS             complement(4018..4908)
                     /locus_tag="VHM69_00515"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2738898.1"
                     /translation="MAGRILVMPERTNSIGRHLPTSGGLGKTLDAARELGCETVQIFV
                     SNPQGWADPVPRKDASVFAERSRELGISPVVAHAKYLINLASKTEELRDLSVRALAHE
                     LVAAGDLGVDLVVVHAGSHGGEGVEKGMDRLVDGLERAREQAARLDGVVAETVVENSV
                     GAGTQLCSTFEALGEVAHRAGVRVCVDTAHAFVAGYDLEAAGGGRSVAAEILSAAGSK
                     VALVHLNDARNELGSHRDGHRKVGEGRIPVDAWPGFFEGLPGVPVVMETPYGTPEADS
                     EEVRLVKKLAGGLRKARVGR"
     gene            4975..5424
                     /locus_tag="VHM69_00520"
     CDS             4975..5424
                     /locus_tag="VHM69_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738899.1"
                     /translation="MAVEGKVAKILGNNEIVINRGSGAGVRQGMLFEIFAPEGEEVWD
                     PDTGETLGTVEDVKARAEVTEVKDRLAVARLHNTQEMPFGAVNIGEMQENLQRMFGQM
                     FGDDVQIRGFGASTGDDTDDLESMMGGPLQDLSKVQVGDAVREIPRR"
     gene            5431..6129
                     /locus_tag="VHM69_00525"
     CDS             5431..6129
                     /locus_tag="VHM69_00525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2738900.1"
                     /translation="MNSLSRLPVTFGLIAACVAVYFAVALAGVGYGYPLNVGLVTQPP
                     AVLAMGALVPASVTDGDVWLLLTSMFLHSGFIHLALNMLSLYFLGSFVEQAFGRGRFL
                     GLYLLSGLAGGIAYLYFGDFHGPVVGASGAIFGLLGGVLGYSLRRGTFSWQNPLIRQL
                     LILTALNLYIGFSVANISNTAHIGGLLGGAAFGWLAAPNVYTRKRLRAATPTVVLLGT
                     ELVLLAIWLLYFAG"
     gene            complement(6143..6868)
                     /locus_tag="VHM69_00530"
     CDS             complement(6143..6868)
                     /locus_tag="VHM69_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX2738901.1"
                     /translation="MNAIVMAVLAATGFSAASLLALLGNELGSRGRSYTAAFAAGILL
                     ALAFVDLFPEGLGHAGEAAVGGFVAGFALLFLTEAFTRAHTHHLPEEHVHKHALGPFV
                     LGLAIHNLADGFVLGVGAEAAAVTSWLVGLGVLVHQMPVGISLAAVLLAARAGKGTIM
                     WTAILLGLAIPAAATLTVLIPVPGDGALGILMGVAGGVLAYIGAAHLLPEAQAEHTNR
                     FAGMVFVATLVITAAGLMALPHG"
     gene            complement(6921..7325)
                     /locus_tag="VHM69_00535"
     CDS             complement(6921..7325)
                     /locus_tag="VHM69_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2738902.1"
                     /translation="MVETALERAVGKLRGGGHKVTPQRLAVLGAIIAEQHQRLEGIMA
                     RCPEVGMVTVYRTLDLFGELGLVRRLDLGDGARYELADDHHHHLICEECGTVDEFEQC
                     PLDLRRLPPQLGSDFEVRAHSVEVYGRCSACR"
     gene            7472..>8113
                     /locus_tag="VHM69_00540"
     CDS             7472..>8113
                     /locus_tag="VHM69_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738903.1"
                     /translation="MATRIQKRLTVLARFRSILSGRDWVYLSSLMVPLVIYNLVLKGI
                     RIRSQEDIEGGFAAFNLIRSDLLFNLGYVCLWVGLFALTKRRRFRRLVVVLFHLTTVV
                     VAATTTSAHHYFQETGSTLSLNVIVYSLSKIGDVKDVIESVASPAIWASIGVILCYIA
                     FGPWLITRLLCGKRDRTRHPGSLAASRWVAIGACGAALGFIFLSLPVDAGGASK"
BASE COUNT         1452 a         2634 c         2478 g         1549 t
ORIGIN      
        1 gcgaacgtcg gcctccgcgg cggccgggtt gcgttgattc ctgccagggt aattttacgc
       61 aaaagagggt gtctcgcatc gtgaaagcgt ctccccgatc cggcgtcggc gggcagcctt
      121 tctttgacgt aacgcgtccc agagcgtgct gcgacggcga ccattatgcc gatcttgcta
      181 tgggtcgcag gcatcgcagc gaggtttgct acggcccacc cgatgtcctg gagctaaggg
      241 atatcgacaa gcccgagatc gaagacgagg aggtgctcgt acgcgttcac gcggctggcg
      301 tggatcgggg gtacggcacc tcatgacggg tctgccgtac gcgaaacgcc tcgcgggcta
      361 cggtctccga gcgcccacga atcctgtttt aggcatggac gtcgccgggg tcgtggaagc
      421 tgtcggcatc gactacaccc aagaggactt cgctgagggc gatcagcgct acgacctgat
      481 gccatgatcg tcctcaagga gctaatcgaa tccggcaagc tcacaccggt gatcgacagg
      541 tcttacccgc tcgccaagct ccctgaggct atccgatatc tcgaagaggg acacgctcaa
      601 gggaagtcgt catcaccgtg tgaggcgcga cacgcggcca gatcctgtcg ccaagatatg
      661 gaagatgcgc tacgctcaat cgctcgtgtg ctcggagtcc tttcgccgct ccttcgcaga
      721 aggaccatct tccggcgggt aacgtactaa caaccaaaaa gatacaccat atgggggatg
      781 cgcaatgttc gtccgcagag catcatgctt gcggttgaaa ggtgtttgac ccgctcgtcc
      841 cctggacggg ataaaggagg ctcgcagcct tgtacgccag gataagtacg cttgcaggtt
      901 cccccgacca gatagacgaa gggctgcgtt acgtccgaga taacgtcctg ccccagctcc
      961 aacaacagga cgggttcaaa ggcatgacta ctctcgccga ccgtgagtca ggcaagacgc
     1021 ctggcataac cttctgggag ggcgaagaag ccctccgagc cagtgaggaa gcggccgaga
     1081 agatgcgcga agactcggct gaggccatgg ccgacacgat agcgagcgtg gagcgcttcg
     1141 aggtcggctt catggcgatg tcgagctata gtcgcagtcc aatcgggtag ggagtcaatg
     1201 gcaagccttc atcttggaca agaataacgt ggctactgcg acaagcgctt tgatccggca
     1261 cggttcgggg ccagtcttga accctcaggt ggactacctg agcctcgaga gccctggcgg
     1321 tacgaaaggt cgacgtgggg cttcgtctac gacgaaggac atgacctcgt ccgctttagg
     1381 gacagccgct tcgccttctc ccgcgagcac gcggaccggg gagctgctta ggttgtggtg
     1441 catgatcgga gaagaggctt ctatgcccgt ggagcatggc aagcagggag tcaatgcatg
     1501 aagttcaggg agaacagagc cgcgggctgg ggcgtgaggg agtttcacaa cgtggagccg
     1561 ccgccccaag ggccgcgccc tgggcacata gacctcgggt ggtcattgaa ggtggtgcag
     1621 aggggcacga agcagaccac tgggcgactt tgggacgtca ctgtctacta tcaggtgggg
     1681 cgcaaaaaac gcgtctatca cggtgcggag gcgaatcgca ttctccagac agtgcaggag
     1741 aatcgctcgg accggcagca accacatccc gcagccgtat cgcaggtgtc acaggcggaa
     1801 taggtgatcc gctaatcgtc taaggtccgg gatgttctag ctgcaatccg gaaggcccgg
     1861 gagccataag cccacgttcg atcgtggtcc gggaatcccg ggaaatgaca gcccacgaac
     1921 gcgttaccga ccaacgggga tgtcaggccg gctcccggac ggcccactat cccagggaca
     1981 attctgttct ctctggcagg cgggcgctct ccctgagtat ggcctccgcc gtttcgtcag
     2041 caagagcatc aagcaactcc tcgtcgaggg ccacatgctc cttgctccgg cagttgcccg
     2101 tccacccctc cactcgtggc cgactgggcg tcccggtttc tgtgtgtgct aacctcttat
     2161 ggtcttatgg agaacctcct cagttcgctc gcgctaacgg tcttcgtcgg tatcctcgcg
     2221 ctgctcgcgc agtggtcgag gaagagccgg agggcggaga tctcactcct agtcgtcgtg
     2281 gtcttcgctt cgctcctgct cctcgtcacg ggggctctgc tcggcgtgtt gtggatctcc
     2341 ggccagatgc cctcaggggt ctatccccgg aagctgttgg ctgtcacctg cgtccccgtg
     2401 gcactggcgg gtctgatcgg gctggcgctc tgcgtccctc cgctaaggaa gatcctgggc
     2461 aacaggccga acggagagtt ctggacggac ccaccgatct tcctcgccct ctggttgttc
     2521 gtcaccgtgc tgcttgcaaa caacctggtc ggcatcctcg ggttcgagca actggagcag
     2581 ggcggcgcct tcgctttggg taacggcggc aggatcccac ccgtcgctat cctggcgagc
     2641 cagttaccct tcgtcgtcct cgcgctcctt ggcgtcggcg cgggcatccg ccgcaccccg
     2701 cgcgagacgc tcgcccgtct cggctacggc cccatctccc tgaaacacct cggcatcgtc
     2761 atgctcttta tcgcgggggc gttcgcgctc tcgctggccg ccgactacct gttctcacag
     2821 gcgcagcccg agctgtaccg caaggtcggc gaggtctcgg gcacgctctt caacccgaag
     2881 ggtctcagcc tagtctcggc gatcctcttc gccttgctga tcggggtcgg cgccagcctc
     2941 ggggaggaga cgctcttccg gggcgccgtg cagcctgtct tcgggatagc ggctacttcg
     3001 gtcctcttcg cctcgatgca cgtccagtat ggtccgtcgc tgctgctcgg ctacatcttc
     3061 ttgctctccg tcggccttgg aatcctccga cgtcacataa acacaacggc gagcttcctg
     3121 gcgcacgcct cctacaacac gcttggcatc cttgcggcct atttcttcgg ggtgtaaaga
     3181 ttagattacg cccgcggggg atcagccccc gcttcgcgcc gcatgagcac ccactccggt
     3241 tcgccgtcct gtgtgggagc gccgaacgtc tcaaccttct ggaagcctgc tctctcgtac
     3301 acgcggatgg cgcggtggtt gaaggacgcg acggtcagcc tgaacgcggg aggcgagtag
     3361 gcctccttcg cgtacttgag ccctgcgcgc acgaactcct ctccgaggcc ccgtcctgcc
     3421 agatcgggcc tcatcccgag tccaacgtcc agcgccgctt cgcgccggta tatatctctt
     3481 ccggcagaaa cacgcgcgtc ctcaccgaag cagaagtatc ccaccaggtc tccgccttcg
     3541 tcgtatacgg agtggtaaag caaacgcggt tcgaggagcg aggcgacgga ggtgggatct
     3601 ccatcgtata ccgcataacg tccgccgtag cgccatcgcg agatggactt tgcgtcgtcg
     3661 ggtgtgatcg gcttcaggga gaagcgcaac gtcggggtct tgggtgtcta ggcggccaga
     3721 gagttggtaa cggggacgac cggcgccggc tcgagggcag aggcgatcct gaccaacagc
     3781 tcgctaacct cgacgtccag tcccctggcg atgctctcga ggaccctgga agaggggtcc
     3841 ttctcaccgc gctcgatctc ggccaggtac gagaccgaga cgtgcgcgcc ctcggcgacc
     3901 tgctttagcg tgagcccgcg ctcgttgcgt tcggcccgca gggtctctcc gatggtggag
     3961 aggaggtctc ccctgtgccg ctccggactt atctccaagg tcttcacaac ctagattcta
     4021 tcttccaacc cgcgctttgc gcaacccacc agccaacttc ttgaccagac gcacctcttc
     4081 ggagtcggct tcaggggtcc cgtagggggt ctccatgacc acgggaactc ccggcagacc
     4141 ctcgaagaac ccgggccagg cgtccacggg aattcgtccc tcccccactt tccgatgccc
     4201 gtctctatgg ctcccgagct cgttcctcgc atcgttgaga tgcaccagag caaccttact
     4261 gcctgcggcc gacaagatct ccgcggcgac cgacctccct ccgccggccg cctctagatc
     4321 gtagcctgcc acgaaggcgt gggccgtgtc cacgcatact cgaactccgg cacgatgcgc
     4381 gacctcccca agcgcctcga acgtggagca gagctgggtc cccgcgccga cggagttctc
     4441 gactaccgtc tcggccacaa ctccgtcgag ccttgcggcc tgctccctcg cgcgctccag
     4501 cccgtcaacg agccggtcca tacccttctc caccccttct ccgccgtggc tgccagcatg
     4561 cacaacgacg aggtcgacgc cgagatctcc tgcggcgaca agctcgtgag ccagggcgcg
     4621 caccgagaga tcccgcaact cctcggtctt cgaggcgagg ttgatgagat acttcgcgtg
     4681 cgcgacgacg ggcgagatgc ccaattctcg gcttctctcg gcgaagacgc tcgcatcctt
     4741 gcgtggaacc gggtcagccc agccctgcgg gttggagacg aatatctgga ccgtctcgca
     4801 cccgagctcg cgagcggcat cgagcgtctt tccgagccca cccgaagtcg gaagatgcct
     4861 tccgatgctg ttcgttctct ccggcatgac gaggattcta ccggccatcg gcggccttcg
     4921 ctgtgagaga tataatccta cttgactcta taacgatcgg aaggagccag ccacatggcg
     4981 gtcgaaggca aggtagcgaa gatcctggga aacaacgaga tcgtcataaa ccgcggaagt
     5041 ggagctggcg tccgtcaggg catgctcttc gagatcttcg ccccggaggg cgaggaggtc
     5101 tgggatcccg acaccggcga gaccctcggc accgtcgagg acgtgaaggc gagggccgag
     5161 gtcacggagg tcaaggacag gctcgcagtg gcccgcctcc acaacaccca ggagatgccg
     5221 ttcggggccg tgaacatcgg ggagatgcag gagaacctgc agcgcatgtt cggccagatg
     5281 ttcggcgacg acgtgcagat ccgcggcttc ggcgcgagta cgggcgacga cactgatgac
     5341 ctagagtcca tgatgggagg tccattgcaa gacctctcga aggtgcaggt cggggacgcc
     5401 gtgcgcgaga tcccacgccg ctagaatcta ttgaactccc tctctcgcct gccggtcacg
     5461 ttcggcctga tagccgcctg cgtcgccgtg tacttcgccg tcgcgctcgc cggcgttggc
     5521 tacggttacc cgctgaacgt agggctggtg acgcagcctc ccgcggtgct cgcgatgggc
     5581 gcactggttc ccgcctccgt gacagatggc gacgtgtggc tgcttctgac gagcatgttt
     5641 ctgcattcgg gcttcatcca cctcgctctg aacatgctct cgttgtactt cctcggctcg
     5701 ttcgtggagc aggcgttcgg gcggggccgt ttcctcgggc tctacctgtt gagcggcctc
     5761 gcaggcggta tcgcctacct ctacttcgga gacttccacg gccctgtggt cggggcttcg
     5821 ggcgcgatct tcggccttct cggaggagtg ctcggttact ccctgcgccg cggcaccttc
     5881 tcctggcaga accccctgat ccggcagctc ctgatcctga ccgccctgaa cctctacatc
     5941 gggttctccg tagccaatat aagcaacacg gcccatatcg gcggcctcct gggcggggcc
     6001 gcgttcggtt ggctagcggc acccaacgta tacacgcgca agagactgcg cgccgcgacg
     6061 ccgacggtcg tcctgctcgg tactgagctt gtcctgctcg ccatctggct cctctacttc
     6121 gccggatagc cgcgccgaag gactagccgt gcggaagtgc catcagccct gcggccgtaa
     6181 tcacgagcgt cgcaacgaac accatgccgg cgaacctgtt cgtatgctcg gcctgggcct
     6241 cggggagcag gtgtgctgcg ccgatgtagg ccagcacccc tccggctact cccatcagaa
     6301 ttccgagcgc accgtcgccg ggcaccggaa tgagcacggt caacgtcgcg gcggcgggga
     6361 tcgcgaggcc gagcaggatc gccgtccaca tgatcgtgcc ttttccggcg cgcgccgcca
     6421 gcaggaccgc ggccagggag atcccaacag gcatctggtg gacgagaact ccgagcccga
     6481 cgagccagga ggtaacggcc gcggcctcgg cgccgacgcc cagaacgaag ccatcggcga
     6541 ggttgtggat tgccagcccg agtacgaacg gcccgagcgc gtgtttgtgg acgtgttctt
     6601 caggcaggtg gtgcgtgtga gcccgtgtaa acgcctcggt aaggaacaac agggcgaacc
     6661 ccgcaacgaa cccgcctacc gcagcctctc cggcgtgacc gagcccctcc gggaagagat
     6721 ccacgaacgc gagcgcgagc agtatcccag ctgcgaaggc ggccgtgtag gatcggcccc
     6781 tggatcccaa ctcgttgcca agcagagcga gaagactggc cgccgagaaa cccgtcgcgg
     6841 ccagcacagc catgacgatg gcgttcatag gcgcaaccct ccacccgcca ccaggcggcc
     6901 acacaccccg atctcaccat ctacctgcac gcggagcacc tcccgtagac ctccacgcta
     6961 tgagccctga cctcgaagtc actccccaac tgggggggta gccgtcgtag gtcaagggga
     7021 cattgctcga actcgtccac ggtcccacac tcctcgcaga tgaggtggtg gtggtgatcg
     7081 tcggccagct cgtagcgcgc tccgtcgccg aggtccagcc tgcggacgag ccccaactcg
     7141 ccgaagagat ccagcgtccg gtaaaccgtc accatgccaa cctccggaca gcgggccata
     7201 atgccctcca gacgctggtg ctgctccgca atgatcgccc cgagtaccgc cagccgctgc
     7261 ggcgtgacct tgtgcccgcc accacgtagc tttcctacgg ccctctcaag cgccgtctct
     7321 accacttttg acctcgctct ttgacgctaa atgaaaagtt ttttcatcta gaacctaaca
     7381 gaagaatcag tagcggtcaa tcttgctgtg ccgggcaagt cgagcggcac ctgtcacgtc
     7441 ccgcgaaatc tttgtaaact cacgcgcagt catggcgacg agaattcaga agcggcttac
     7501 tgtactggcc cgcttccgct ccatcttgag cggtcgcgac tgggtctacc tgtccagcct
     7561 gatggttcca ctcgtgatct acaatctggt gttgaagggg atccggatac gctctcagga
     7621 agacatcgag ggaggattcg ccgcgttcaa cctgatccga tcggaccttc tcttcaacct
     7681 cgggtacgtt tgcctgtggg tcggtctgtt cgccctgact aagcggcgac gtttccggcg
     7741 gctggtggtc gtactcttcc atctgaccac cgtagtggtc gcggccacga cgacttcggc
     7801 gcaccactac tttcaggaga ccggctcgac gctcagcctc aacgtcatcg tgtactcgtt
     7861 gagcaagatc ggcgacgtca aagacgtcat cgagagtgtg gcttccccgg ccatctgggc
     7921 gtccatcggg gtgatccttt gctacatcgc gttcggtccc tggctgatta ccaggctcct
     7981 gtgcggaaag cgggacagga cacggcatcc gggttctctg gctgcgtcca ggtgggtcgc
     8041 aatcggggca tgtggcgctg cgctggggtt catcttcctc tcgctgcccg ttgacgcggg
     8101 tggggccagc aag
//
LOCUS       DATAIR010000021         9276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_278111, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000021 DATAIR010000000
VERSION     DATAIR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278111"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /gene="ligA"
                     /locus_tag="VHM69_00545"
     CDS             complement(<1..1507)
                     /gene="ligA"
                     /locus_tag="VHM69_00545"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2738904.1"
                     /translation="MDLRAYNSCVAGGVDVRGRIEELREQVRYHNRRYHVEDAPEISD
                     AEYDALYRELEALEDEHPELVTPDSPTQRVGGEPIEGFQEIRHSVPMLSLANARKVED
                     LREWDARVRRLLGEDETPRYVTELKIDGLAVSLRYENGRLVRGSTRGNGVVGEDVSAQ
                     LRTIRSIPDRLDDDPPEVLEPRGEAYIPIADFEKLNKQLEEEGKKTYANPRNLAAGSI
                     RQLDPRMTARRPLTILLYGVGEGGEIFESHSAMLSALKRYGLRVNPYEVHDDIDSVIE
                     ECERWARKRESIGYQIDGVVVKVDSREQQLALGSVARSPRWAIAYKFEPLAGRTKLID
                     IHVKVGRTGALTPQAVLEPVNVGGVVISRATLHNEDYVKEKGILIGDTVVVERAGDVI
                     PQVVEAVTEERDGDERAFEMPKDCPVCGEPVSRPEGEAVTRCVNARCPAQALEHIIHW
                     SSKSAMDIDGLGEKLATRLFDLGFIRDVADVYDLKVEQLVPLEGFGEKSAQN"
     gene            1589..1936
                     /locus_tag="VHM69_00550"
     CDS             1589..1936
                     /locus_tag="VHM69_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738905.1"
                     /translation="MPLESFELALEEDMSLSEEFDLPLTILVVRAKDRLDSQTTRRLL
                     DVLRTAEPATLPTPSELAVVLPNTGPDSARAVESRALEVLPDAGVGLAVYRPGDAVPD
                     LLGRARSAAGEEP"
     gene            complement(1964..2440)
                     /locus_tag="VHM69_00555"
     CDS             complement(1964..2440)
                     /locus_tag="VHM69_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEX2738906.1"
                     /translation="MVLLSLLLAACSAAGNKDSPKEKTTEPRNLRVVRIFSGAPGQGP
                     QGPRVIVAPSATTLSKELGAEIPDSGEGTYLISYWGEKPTGGYSLAIESARLEGDQVT
                     VRLKLKEPPPDAMVTQSLTYPYAAAVVRDVSLQGKTFSFVDQNGRKLDWPVRRVSG"
     gene            2902..3360
                     /locus_tag="VHM69_00560"
     CDS             2902..3360
                     /locus_tag="VHM69_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738907.1"
                     /translation="MRRTATILAAIALMMTFSSGVALAAYQDSITGTGHTDILSGTRQ
                     AEQISGLGGGDQINGGGGTDLVKGGLGPDELSDGLGRDTVYGGAGVDNVIGYGDTSVD
                     YFYAGGGDDTVQSRDVPAVKDVVDCGGGTDTVYADEADVVGGDCERVRTR"
     gene            complement(3495..3800)
                     /locus_tag="VHM69_00565"
     CDS             complement(3495..3800)
                     /locus_tag="VHM69_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738908.1"
                     /translation="MMENDHRPDNPYTQMEGYQVSDASGEAVGKVEYTVYDAPSDVLK
                     YVVVNGRPIPAERIEVDPEEQRVLVLYDAAVVASAPEMEEPSGAFDETVREHYEGRG"
     gene            3852..4850
                     /locus_tag="VHM69_00570"
     CDS             3852..4850
                     /locus_tag="VHM69_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738909.1"
                     /translation="MGGRTDQGRNARSLKAFSFLLLALMVASLLLVHTASYEPAVSYI
                     LLYLVSSVGIITAAVVVPLLPTEQFTAAFCGSIYALYAAVVAMMVFFSGGVSSELYVL
                     FFPLLLSSALHGSWRVVLAVLAAVLFCYALAMMPALLEEGTGQEIASLVFYRLAAVGL
                     TGVFLIYVARGVITAGTAEDYATDDDGSVLLERVEEELKARRGVPVAVILVDPGGDME
                     AMELLLERVWSRIGEPVLLGEGNLFGVVVSGADDGGVESAARRVLAAASSLGAQDTRA
                     GAAIYPRDARSAEDLLIAAGRALEAAFEIESPSAIVLAGRGTPRPDGEAGRYQAAR"
     gene            5157..5909
                     /locus_tag="VHM69_00575"
     CDS             5157..5909
                     /locus_tag="VHM69_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX2738910.1"
                     /translation="MGRFTRAIRGFFGRFLSGVEQRNPAILLENAVQDELENLKKLQV
                     AAARTMAYEKMLSNDAVSKQKILAVKERQARELASRGQREAAIEVVQQKQEAQTSLID
                     IEARLEEARKNSQEMEQAFRDQERRYNDVVRERAALMEEHQRSRALRTANEARSNISL
                     SDSSRDLESARQAIRQASYEAEAIGELGTSDTERQIAAAEIDIKRLDAERELEMIEVQ
                     MGLREPERIEAAPTEETTEPQVEENEEGPRGQ"
     gene            5899..6690
                     /locus_tag="VHM69_00580"
     CDS             5899..6690
                     /locus_tag="VHM69_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738911.1"
                     /translation="MASRQNLSRRLMIRLAPFGTFAGLGAFAALEANYILAGLLGIGA
                     VATGKRLLNRRENARKELLSRARENARELGRVAREDRIAAAQMKRLAALQEGLIESWE
                     LLPEECRPLLDEDIFTIIEEVEGAARLARRRAALRRHLESVDRRSISRRIKELQKDLA
                     VLNPGSPMRAPFESALAGRRGELAGYDDILGGISMINAQLESAESLLGNLRGELLALD
                     DSLVSGSLDPGLAQLKERIAYFRRGLDEVTRRVETLPATERMSAR"
     gene            6687..7346
                     /locus_tag="VHM69_00585"
     CDS             6687..7346
                     /locus_tag="VHM69_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738912.1"
                     /translation="MNEDRSTTRQSFFSRVFAGSIEGEGTAIYSSEEVGASSAGELES
                     SSAHGFTVERAAEVIRNLPPEVPRPAAVRIVRGTLEAAGIDIAELESSTRARESKLNS
                     EIDLSEGRIQQLKDETEEVIRNLEDEIRKAREARNFGVSEEERKIASAEVGLDNIDMV
                     REFFGLPQEEGTPPSDPDAGEETQVMDSVDEEDDTQILRRPGPLSDSEDYLDSRENKD
                     K"
     gene            7343..7837
                     /locus_tag="VHM69_00590"
     CDS             7343..7837
                     /locus_tag="VHM69_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2738913.1"
                     /translation="MSISRRLGRLAQGFVSSLQEDERFRERLRASRERSEALRDAFEA
                     GWRGASEEWRRAEERRAAEAAAAENERNSTSEGRNPGWRATATTFVPRRYPPNVLAAY
                     DRLGLVPGASMEEVDKRRRDLVKRFHPDRFSDPEKRVRAERVTSEINAAHDAIERHVL
                     RKSR"
     gene            8011..8577
                     /locus_tag="VHM69_00595"
     CDS             8011..8577
                     /locus_tag="VHM69_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2738914.1"
                     /translation="MVLRRFNRADLDHLFVLHNDPEVMRYLNGGKPTTRREIGHEYHT
                     RFVRDGYWTAIEKATGDFLGWFGFHPTESRGTDEFELGYRLKRLVWGKGFATEGSRAL
                     IRKGFTELGVRRVVAETMAVNLASRRVMEKSGLTHVRAFHEDWSDPIAGAEHGEVEYE
                     ITRAAWERQDAEGCWWTSEENGTGYSPG"
     gene            8535..9065
                     /locus_tag="VHM69_00600"
     CDS             8535..9065
                     /locus_tag="VHM69_00600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2738915.1"
                     /translation="MDIRREWDRIFSGLNEGAGSEDAWLERWQVMLETSREIPILELG
                     CGGGHDAAFLTEIGFAVIATDLSDEALELTRRRAPDARVENVDLTRGLPFPDAAFRVI
                     VASLSLHYFPWRKTLEILDDIHRCLEPQGHLLARFNSTRDPRYFAAEKQQIEDNFYLI
                     GGCPNVSSTGRVCMCC"
BASE COUNT         1707 a         2849 c         3087 g         1633 t
ORIGIN      
        1 ggttctgcgc gctcttctct ccgaagcctt cgagcgggac gagctgttcg actttaaggt
       61 cgtatacatc ggccacgtct cttatgaagc cgaggtcgaa gaggcgggtc gcgagttttt
      121 cgccgaggcc gtcgatgtcc atggctgact tcgaggacca gtggattatg tgttcgaggg
      181 cctgcgcggg acagcgggcg ttcacgcagc gggttacggc ttcgccctcg ggacgcgaga
      241 caggctcgcc gcagactggg cagtccttcg gcatctcgaa ggcgcgttcg tctccgtcgc
      301 gttcctctgt aacggcctcg acgacctgcg ggatcacatc tcccgcccgc tcgacgacga
      361 ccgtgtcgcc gatgaggatg cccttctcct tgacgtagtc ctcgttgtgc agcgtggccc
      421 gcgagatcac gaccccaccg acgttcacgg gttcgagcac cgcctgcggc gtgagcgcgc
      481 ccgtccgacc gaccttaacg tggatgtcga tcagcttcgt acgtcccgcg agcggctcga
      541 acttgtacgc tatcgcccac cgcggagacc tggcgacgga gccgagggcg agttgttgct
      601 cgcgcgagtc gaccttcact acgacgccgt cgatctggta gccgatcgac tcccgcttcc
      661 ttgcccaccg ctcgcactcc tcgatcacgg agtctatgtc gtcgtggacc tcgtatgggt
      721 tgacgcgcag tccgtatctc tttagcgcgg agagcatcgc cgagtggctc tcgaagatct
      781 cacccccctc cccgacgccg taaagcagta tggtcagggg acgccgggcc gtcatcctcg
      841 ggtcgagctg cctgatggag cccgccgcca ggttgcgcgg gttggcgtag gttttcttgc
      901 cttcttcttc gagttgcttg ttgagcttct cgaagtctgc gatggggatg tatgcctcgc
      961 cgcgcggctc gagaacctcg ggcggatcgt cgtcgaggcg gtcggggatc gaccgtatcg
     1021 tcctgagctg cgcgctcaca tcctcgccga ccacgccgtt gccccgagta gagcccctga
     1081 ccaaacgtcc gttctcgtag cgtagcgaga ccgcgaggcc gtctatcttc agctccgtca
     1141 cgtagcgcgg ggtctcgtcc tcgccgagca agcgccgcac gcgggcatcc cactccctca
     1201 gatcttccac cttgcgggcg ttggcgaggg agagcatagg gacggagtgc cggatctcct
     1261 gaaacccttc gatgggctcg ccgccgacgc gctgggtggg agagtccggg gtgacgagct
     1321 cggggtgctc gtcttccagg gcttcgagtt ctctatacag ggcgtcgtac tctgcgtcgg
     1381 agatctccgg cgcgtcctcg acgtggtagc ggcggttgtg gtagcggacc tgctcgcgca
     1441 gctcctctat cctgccccga acgtctacgc ctccggccac gcaggaatta tacgcacgga
     1501 ggtccatgct cccaagggga cgccgataag aggcggaccc cggcgtataa ttcctgccat
     1561 gggtgagcga cgccgcgcaa aggagccttt gcccctcgag tccttcgagc tggccctgga
     1621 ggaggacatg tcgttgtccg aggagttcga tctccccctg accatcctgg tcgttcgcgc
     1681 gaaggacagg ctcgactcgc agacgacccg ccggttgctc gacgtgttgc gcaccgcgga
     1741 accggcgacc ctgccgaccc cctccgagct cgccgtggtg ctgccgaaca cgggccctga
     1801 cagcgcccgc gctgtcgaga gccgggcgct tgaggtcctc cctgacgccg gggtcggtct
     1861 cgccgtatac agaccggggg acgcggtccc cgaccttctc gggcgcgcaa ggagcgcggc
     1921 cggggaagag ccctaaagca gtcagcatgc tctggccttc gctttagccg gatacccgtc
     1981 ggacaggcca gtcgagcttc cggccattct ggtccacgaa ggagaatgtc tttccctgaa
     2041 ggctcacgtc tcgcacgacg gccgcagcgt aggggtaggt aagggattgt gtcaccatcg
     2101 cgtccggcgg gggctccttg agcttcagtc ggaccgtgac ctggtcccct tcgaggcgcg
     2161 ctgattcgat ggcgagcgag tagccgccgg tgggcttctc gccccaatag gagatcaggt
     2221 aggtcccttc tccagagtcg gggatctccg ctccgagttc cttcgaaagc gtcgtggccg
     2281 agggagcaac gattactcgc gggccctgcg gaccctgtcc cggggcgccg gagaagatcc
     2341 tcactacgcg gaggttgcga ggttccgtgg tcttctcctt gggagagtcc ttattacctg
     2401 cggccgaaca cgcggcgagc agaagcgaga gcagaaccaa gcttgcgcga gccattgtgc
     2461 ggagagctta caccatgcgg cgccgcctga gccaggcgat gcccgttcgg agattcgagc
     2521 ctggcgaagt gcctgtcggt ctcctcgcgc aggagccggc ggtagtccac tctgacccca
     2581 gggcagaagc agccgcatcc cgaccaggta cagtacgtgg aggttaaaac gattacgatg
     2641 cgattgatcc agcgccgtat cctgcctacg gcgtgccgct cgcggaagct ctccgcgtaa
     2701 ggatctcggc gctacactca aaacgctcac tcaggacaca aggttgcgcc cacccgatgc
     2761 gatacgcaac ctctctccga tcccgtgtgc cgttaccgaa acgttataag accgtggcct
     2821 aaagtttatt catctgtagc cttgccgtta cccgccctct ctaaagtacc tactgtagac
     2881 cggcgagaaa aggggagaca gatgaggcgg acggcgacga tactggcggc aatagccctc
     2941 atgatgacct tctcctccgg cgttgccctg gcggcctacc aggactcgat cacaggcacc
     3001 ggccacaccg acatcctctc cggcaccagg caggcggagc agatctccgg cctcggtggc
     3061 ggcgaccaga tcaacggcgg cggcggtacc gacctcgtca agggcgggct gggtcccgac
     3121 gagctcagcg acgggctcgg acgggatacc gtctacggcg gcgccggcgt ggacaacgtg
     3181 atcggctacg gtgatacctc cgtggactac ttctacgctg gcggcggcga cgacaccgtc
     3241 cagtcgaggg acgtacccgc cgtcaaggac gttgtcgact gcggcggcgg taccgacacc
     3301 gtgtacgcgg acgaggccga cgtcgtcgga ggcgactgcg agcgggtgag gaccaggtaa
     3361 ggtcttccgg ctcggtctca cgaacacagc ggagggcccg gatacagatc caggcccttc
     3421 gtcttcatgc acgcatctct agagatcacc gtaacggaga cttacgtgat gagattggat
     3481 cccggttcac ccctctaacc gcgaccctcg tagtgctcgc gtacggtttc gtcgaaagct
     3541 cctgaaggtt cctccatctc gggcgctgac gctacaaccg ccgcgtcgta gaggaccagg
     3601 acgcgctgct cttcggggtc aacttctatt cgctcggcgg ggatgggacg tccattgacg
     3661 acgacgtact tcaggacatc gctcggggcg tcgtagacgg tgtattcgac cttccctacc
     3721 gcctccccgg aggcgtcgct gacctggtat ccctccatct gcgtgtaggg gttatccggg
     3781 cggtggtcat tctccatcat ggttcgtcct ccggtggcag gcatgcgcgt atgtgcgtat
     3841 gatacttcgg cgtgggcggc agaacagatc agggtcgcaa cgcccggagc ctgaaggctt
     3901 tctcattcct gctccttgcc ctgatggtgg cctcgttgct gctcgtgcac acggcctcct
     3961 acgagcctgc tgtctcctac atcctgctct acctcgtctc ctccgtaggc atcatcaccg
     4021 cggcggtggt ggtcccgctg cttcctacgg agcaattcac cgccgctttc tgcggctcga
     4081 tctacgccct gtacgctgcc gtcgtcgcca tgatggtctt cttctcaggg ggggtatcga
     4141 gcgagctgta cgtgttgttc ttcccgctac tcctgtcctc cgcgctgcac gggtcgtggc
     4201 gggtggtgct agcggtcctc gccgccgtcc tgttctgcta cgccctggcg atgatgcccg
     4261 ccctcctcga agaggggacg gggcaggaga tcgcctccct cgtcttctac aggctcgccg
     4321 ccgtcggcct gaccggagtg tttttgatct acgtagcaag aggcgtaatc accgcaggga
     4381 ccgcggagga ttacgcaacg gatgatgacg ggtccgtact gctcgagcgg gtggaggagg
     4441 agctcaaggc gcgcaggggt gtgcccgtag cggtgatcct ggtcgacccc ggaggcgaca
     4501 tggaggctat ggaactgctc ctcgagaggg tgtggtcgcg tatcggggag ccggttctgc
     4561 tgggtgaggg caacctcttc ggggtggtcg tgagcggggc tgacgacgga ggagtagaga
     4621 gcgcggcgcg gcgcgtactg gccgcggcga gctctttagg cgcgcaggat acaagggccg
     4681 gggcggcgat ctacccgcgc gacgcccgct ccgccgaaga cctcctgatc gccgccggac
     4741 gcgcgctcga ggctgctttc gagatcgaat cacccagcgc catagtcctc gccggcaggg
     4801 gtacgccacg accggacggc gaagccggcc gctaccaggc cgccaggtag ctttcagctc
     4861 gctccggcta cgcctccgct tgtcagcatc tcagcacgcc gcctgacggc ggctctcagc
     4921 atttcagccg ggttgtggcc ggccatccga agctcaagca cggatgaagc tgaccgctga
     4981 aagcgagggc ccgtagggcc gtgctgagag cgaagcccga agggcgtagc gtgctgacaa
     5041 gccgcgaagc ggcatgctga cagctccttt gaaacccttt ctgaggtcgt acgtaccgat
     5101 gatataatcc acgacgtact gtatagacgt gacgacgccg ggggtgggcg gcacagatgg
     5161 gaagatttac gagggcaatt cgcggtttct tcgggaggtt tctctccggg gtcgaacagc
     5221 gcaacccggc gatactgctc gagaacgcag tccaggacga gttggagaac ctgaagaagc
     5281 tccaggttgc ggcggcccgc acgatggcct acgagaagat gctgtccaac gacgcggtct
     5341 cgaagcagaa gatcctggcc gtcaaggagc gccaggcccg cgagctcgcg agcaggggcc
     5401 agcgggaggc cgccatcgag gttgtccagc agaagcagga agcccagacg agcctgatag
     5461 acatagaggc gcgtctcgaa gaggcccgca agaactccca ggagatggag caggcgttcc
     5521 gcgaccagga gcgccgatac aacgacgttg tgcgcgagcg ggcagccctc atggaggagc
     5581 accagcgctc ccgcgccctc aggaccgcga acgaggcccg ttccaacatc tccctctctg
     5641 actcctcgcg cgacctcgag agtgcgcggc aggcgatccg ccaggcctcc tacgaggccg
     5701 aggcaatcgg cgagttgggc actagcgaca ccgagcgcca gatagcagcg gccgagatag
     5761 acataaaacg cctcgacgca gagcgggagc tggagatgat cgaggtccag atgggcctgc
     5821 gcgagccaga acggatcgag gcagcaccga ccgaggagac tacggaacct caggtcgaag
     5881 agaacgagga gggcccgcgt ggccagtaga cagaacctct cgcggcggct gatgatacgc
     5941 ctggctcctt tcggtacgtt cgcggggctc ggggcatttg cggcactcga agcaaattac
     6001 atcctcgctg gactactcgg cataggggcc gtagcgacgg gcaaacgcct cctcaacagg
     6061 cgggagaacg cgcgcaagga gcttttgagt cgggcgaggg agaacgccag ggagctcggc
     6121 cgggtggcga gggaggaccg catcgccgcg gcgcagatga agcggctcgc ggccctgcaa
     6181 gagggtctca tcgagagctg ggagcttctg ccagaagaat gcaggccgct cctggacgag
     6241 gatatcttca ccatcatcga ggaggtcgag ggcgctgcgc gcctcgcgcg caggagggcc
     6301 gcgctcaggc ggcatctgga gagcgtggac cggcgttcga tctccaggcg catcaaggaa
     6361 ctgcagaagg acctcgccgt tttgaatccg ggatcgccca tgcgtgcccc gttcgagagc
     6421 gcgctcgcgg ggcgtcgggg ggagctcgcg ggatatgatg acatactcgg tggtatcagc
     6481 atgatcaacg cccagctcga gagcgcggag agtctgctcg gcaacttgcg cggcgaactg
     6541 ttggccctgg acgatagcct agtatcaggg tcgctcgacc ctggcctcgc gcagctcaaa
     6601 gagcggatcg catacttccg ccgcgggctc gatgaggtga cgcggcgcgt cgagacccta
     6661 cccgctacgg aaaggatgtc ggcgagatga acgaggatcg cagcactact cggcaaagct
     6721 tcttctccag ggtgttcgca gggagcatcg agggagaggg gaccgcaatc tactcctctg
     6781 aagaagtcgg cgcgtcgtcg gcgggcgaac tggagagttc atcggcgcac ggtttcaccg
     6841 tcgagcgggc cgccgaggtc ataaggaacc tcccgcccga ggtgccgagg ccagccgcgg
     6901 tgcgtatcgt gcgtgggacc ctcgaggcgg cagggataga tatagccgag ctcgagtcct
     6961 cgacgagggc cagggagtcc aagctcaact ccgagataga cttgagcgaa ggccgcatac
     7021 agcagctcaa agacgagacg gaagaggtga tccggaacct cgaggacgag atccgcaagg
     7081 cgcgcgaggc gaggaacttc ggcgtctccg aggaggagag gaagatcgcc tcggccgagg
     7141 tcggcctcga caacatcgat atggtgcgcg agttcttcgg gctgccccag gaggaaggca
     7201 ctccgccttc cgaccccgac gcgggggaag agacgcaggt catggatagc gtggatgagg
     7261 aggacgacac ccagatcctc cgtcgtccag gtccgctctc ggactctgaa gactacctgg
     7321 acagcaggga aaacaaggat aagtgagcat ctcgcgccgt cttgggcgcc tcgcgcaggg
     7381 tttcgtctcg agcctgcagg aggacgagcg cttccgagag aggctccgcg ccagccgcga
     7441 acggagcgaa gccctaagag atgcgttcga ggccggttgg aggggtgctt ccgaggagtg
     7501 gcgcagggcc gaggaacggc gcgcggcaga agcagccgcg gcggagaacg agagaaacag
     7561 cacctctgaa ggtcgaaacc cggggtggcg tgccacggca accacgtttg tcccaaggag
     7621 gtacccgccc aacgtcctcg ccgcctatga caggctcggg ctggtccccg gcgcatccat
     7681 ggaagaggtg gacaagaggc gcagggacct agtcaagcgc ttccaccccg accgtttctc
     7741 agaccccgaa aagcgtgtgc gcgccgagcg cgtcacctcg gagatcaacg cggcccacga
     7801 cgccatagaa cgccacgtcc tccgcaagtc gcgctaggac aaatttcgga gagcattccg
     7861 cgggccgaac cgtctcgacg gtcgccggcc ggctgccttg ttatcatggt cgctcctgtt
     7921 tagaagaaat cgtacataac ggcttgctgc cggtacgtcg tttcccgaga gaaggatcag
     7981 gatatacacg tctttctgaa gacggaccgg ttggtactac gccggttcaa cagagccgat
     8041 ctagatcacc tgtttgttct ccataacgac cccgaagtga tgcgatacct taacggtggc
     8101 aagccgacta ctcgcaggga gatcgggcac gaataccata cgcgtttcgt gagagacggc
     8161 tactggacgg ctatcgagaa agccacggga gatttcctcg ggtggttcgg tttccatcct
     8221 acggaaagcc gtggaaccga cgagttcgag ctcgggtacc ggctcaagag attggtctgg
     8281 ggcaagggct tcgcgaccga aggctcgcgt gccctgatcc gcaagggttt caccgagctc
     8341 ggcgtgcgaa gggtggtggc tgagaccatg gcggtcaacc tcgcctccag gcgggtgatg
     8401 gagaagtcgg gcctgacgca cgtgcgagcg tttcacgaag attggtccga tcctatcgcg
     8461 ggggctgagc acggcgaggt cgagtacgaa attacgagag ccgcctggga gcgacaggat
     8521 gcggagggtt gctggtggac atcagaagag aatgggaccg gatattctcc gggttgaacg
     8581 aaggggcggg cagcgaggat gcctggctgg agcgctggca agtcatgctg gagaccagcc
     8641 gcgagatccc gatcctggaa cttggctgtg gcggcggaca cgacgccgcc ttccttacgg
     8701 agatcggttt cgccgttatc gccacggatc tctcggatga ggcgctggag ctcacgcggc
     8761 ggcgcgcgcc ggacgccagg gtggagaacg tggacctcac ccgagggctg cctttccccg
     8821 acgccgcttt ccgagtgatc gtggcgagcc tttcgttgca ctacttcccc tggcgcaaga
     8881 cgctggagat cctggacgac atccaccgct gcctggagcc gcaaggacac cttctggccc
     8941 gcttcaactc gacgcgagac cctcgatact tcgccgcaga aaaacagcag atcgaggata
     9001 acttctatct gatcggggga tgcccaaacg tctcttcgac cggacgagtt tgtatgtgct
     9061 gttagagaac ggctgggatg tggcggaagc cgacgagcgc gtaacgcgca ggttcggcgg
     9121 ggagtaggtg gtgtgggagg tcgccgccag gaaagctgac ggctgaaagc gaaggccgat
     9181 aggcctgagc gtgctgagag ccgccctgcg ggcggcgtgc tgacaagcta agaaaccagt
     9241 tcgccgcgga ctacgagacg gtactcgaag gtcggg
//
LOCUS       DATAIR010000022         9282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_185117, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000022 DATAIR010000000
VERSION     DATAIR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185117"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHM69_00605"
     CDS             <1..220
                     /locus_tag="VHM69_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX2738916.1"
                     /translation="LGYAIGSGSACSSGGHKASRVLLAMGLPERDAFSVVRITVGKEN
                     TAEDVEGFLAAFSTAVAQLRELSPLYAG"
     gene            233..1696
                     /gene="mnmA"
                     /locus_tag="VHM69_00610"
     CDS             233..1696
                     /gene="mnmA"
                     /locus_tag="VHM69_00610"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2738917.1"
                     /translation="MSDRYTKQVIDHLVRPRNAGEILDPSGRGESGDASCGDVACFTV
                     GISENVLEEVRYKVYGCAACIAAGSALAELVEGKHLLDAARVSKADLEDALGGALPAG
                     KGHALTLVLDALHKAFEDHWNSTAGEMLFDGERGKGNGGKTVVAAMSGGVDSAVTALL
                     LKEAGYGVATVTFRLHDGERGSRSCCSPDTVLFARDTAHRMGLPHFTLNLKELFQRRV
                     MQDFVGSYKEGRTPNPCVACNAHVKFHASSFLADRLGFHYVATGHYARVEEGPTLARP
                     VDESKDQTYVLWPVPRDLLSRTLFPLGEYRKTEVRAIAEERGLAVAYTPESQDICFIP
                     DGNYRKFVRKKVEAEPGDVVDAGGQVLGRHAGVVDFTVGQRRGIGVSAPTPLYVTEVR
                     PRSGQVVVGRRQDLEVQEVRVRDLNWFLDPREARFVQVRYNGSAVPCTVEEAADGDWI
                     AHLDEAVMGVAPGQSAVFYTGDGQRVVGGGVVARRGY"
     gene            1766..2143
                     /locus_tag="VHM69_00615"
     CDS             1766..2143
                     /locus_tag="VHM69_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX2738918.1"
                     /translation="MDIFVELMKGLLFAGIAVAFFVLAWMFLRLARIFSTTEQTIKDV
                     SGQVVPLLGKTHVTVDNINSQLSQLDDAVGDVAGMTGELDQTTAVITRGVRGGVIKVS
                     SATAGISRGISTFLGGEKRGEEE"
     gene            2143..2466
                     /locus_tag="VHM69_00620"
     CDS             2143..2466
                     /locus_tag="VHM69_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738919.1"
                     /translation="MASWGRVVAGVVIGVAGTIYATNEDVRKNLPKAARDLPDNVRRR
                     YKSAVSAAREASARRREEILRDLESHGGGHHTARGAPPDPDAATVTQEEAASGEETTE
                     VPEQR"
     gene            2480..2764
                     /locus_tag="VHM69_00625"
     CDS             2480..2764
                     /locus_tag="VHM69_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738920.1"
                     /translation="MTEDHSKEYAYLRLPPHEELRSCVGLVLAGMAARARVGVGGLEE
                     AVEVLEGFHAGDAPTRFRFSLAGEGVLAEVEERAADGSGPRWRTVVELLS"
     gene            2791..3573
                     /locus_tag="VHM69_00630"
     CDS             2791..3573
                     /locus_tag="VHM69_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX2738921.1"
                     /translation="MRTRRLLVRYHKHGDKNAREKVIQEQLPLAEFLARKFSGRGEPL
                     DDLVQVASVGLIKAVDRFDVDRKIEFSTYATPNILGEIKRYFRDKGWAMRVPRGLQEL
                     RQSAKETIRNETVRTGQSPTLHDLAESLDADVESVAEALTLGRAYNTASLDAPVSQDE
                     PDGDTIMDLQADDNSPIEGLEDRILLQEAIEGLRGQQQQILKLRFNEGKTQTEIADHI
                     GVSQMHVSRLLRRALEDLRVELEQLERDRETETLSRDGAHAE"
     gene            3563..6190
                     /gene="alaS"
                     /locus_tag="VHM69_00635"
     CDS             3563..6190
                     /gene="alaS"
                     /locus_tag="VHM69_00635"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX2738922.1"
                     /translation="MQSSEIRRRFLDFFIERDHRFYPSSSLIPHNDPTVLLTTAGMQQ
                     FITYFTGQDRPPTPRAASAQKCFRTQDIEEVGDPSHLTFFEMLGNFSFGDYFKKEAIE
                     WAWEFLTEELGIPPERLWITIFEGNEDAPEDLEAKELWMGVGVPEHKIFGLPKSENWW
                     GPPGDSGPCGPCSEIYYDYGEEYAKGDPSEDPRYGPGGDEGDPRFLEIWNLVFNQYEQ
                     RKDGSLVPLAKTGIDTGLGLERTTAAVQDVLYVYETDVYAPILEKIREYKGVTLGESE
                     ATDRALYIVADHARGIAFLIADGVRPGNQRREYVLRRMIRRAMLQAYTQLGMTPEQLG
                     GVAEVVVDYIGDFYEELRVAREDIRRVVTGEAARFVEIYESGMSLLNAEIERVQGEGF
                     PGDVAFTLHDTYGFPVEVTREVLAERGLSLDEAGFEAAMDAQRERARDAMQGYDRVVA
                     AFRDQEIRSRFVGYEREQVETRIVAIEPVPDAEGEVYVVVAENPFYATGGGQIADEGW
                     ITSEDGQLEVFDSIPAGDYQVLRARVERGSFEAGDAVTASINRVRRQQIEANHTATHI
                     LHWALRAVLGQDVVQAGSYVGPDRLRFDYRYSGKVTEDEIGRVQELSLLKITENQPVR
                     YYTTTLEEARNLGAIMLFGEKYGDLVRVVEIDGFSRELCGGTHVRGTAEIGAFKILSN
                     RKHGADLYRIEVITGREALYYLTGATERAEEISEALKVEIDELPEAVGRLREEVRTAR
                     EAAREQTLKQGLEEVGDLVEGAQQVNGTRVVTGQVVAADVRGLRQISDDVKNRLGGPS
                     VVVLAAALEGKAVLVANLHPEVSQRVEAGEIVREVSGVLGGGGGGGATMAQAGGGNLD
                     AIPDALAKVREILDRRLAE"
     gene            6224..6616
                     /gene="ruvX"
                     /locus_tag="VHM69_00640"
     CDS             6224..6616
                     /gene="ruvX"
                     /locus_tag="VHM69_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX2738923.1"
                     /translation="MAALDLGEVRTGVAVSDPGRILATPLEVIPSVDLAAYLHRLFDE
                     EGISEVVVGVPRTLGGEIGSQARSVLDTLESLRTEFPGVRFVEWDERFTTRMASAGRG
                     GNKKGAVRVDHLAAARMLQEYLEARGRL"
     gene            6613..7728
                     /gene="mltG"
                     /locus_tag="VHM69_00645"
     CDS             6613..7728
                     /gene="mltG"
                     /locus_tag="VHM69_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX2738924.1"
                     /translation="MRRHDKSKRRDREEVEARLDALDLSDSRRSRRKRRSNVGPTALG
                     VLLLIAVLGSVYLIYSAATGGQNGPDQAGVARVEVEKGDTLSDVANKLEEAGVIKNAF
                     VFKLQAKYEGYGTEIKTGRYTFEQGQDSEVILQKLTAGRAAPMIAFTIPEGLTLEETA
                     RTVAADSHVPASRFEEAARRTDYGYAFLDNPDIKTAEGYLFPARYEFEEGVTARQIVD
                     RLLEQYLLETQDLDIAEAEERLGISEHQLVTVASLIEKEAATPKEKPIIASVIYNRMD
                     KGTPLQIDATIQYALEEPKENLSLADLKVDSLYNTYEHKGLPPGPICSPSRESLQAAL
                     NPKETNYLYYVLRADGQSHFFTSNYDDFLRAKEAAGR"
     gene            7993..>9282
                     /locus_tag="VHM69_00650"
     CDS             7993..>9282
                     /locus_tag="VHM69_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2738925.1"
                     /translation="MPAAQRGVGEILVSEGVITGHQLEEARRLGSQQDVGRMLLTLGY
                     VDQADLARALARRLRLEFVDLSVKDVDPGAVGLVERKVLRRYGMLPLRVEGGRLVVAM
                     KDPTNLYALEDLSMLSGYPVSPVVVAESDLRRAFGELFGMGDEVAELLDEAGSGQPAE
                     DSQELELREDQGASGAPTVRLVGTILRRAAGDGASDIHIEPRSGDLAVRFRVDGVLRE
                     VMSVPPALRSGVIARLKVLASLDIAERRLPQDGRFSVKLDDRNMDLRVATLPTTFGEK
                     VVLRLLDNANLHADLRRLGFSSKMLQGYEAVFRRPYGTILVTGPTGSGKSTTLYATLG
                     EINAPEKNIITVEDPVEYRMRGINQVQVNPRIGLTFPSGLRSILRSDPDVVMIGEIRD
                     RETARTSIEAALTGHLVLATLHTNDAPAAVTRLNDMGV"
BASE COUNT         1882 a         2766 c         3092 g         1542 t
ORIGIN      
        1 tctcggctac gcaatcggga gtggatcggc ctgctcttcg ggcgggcaca aggcttcgcg
       61 ggtactgctg gcgatgggcc tgcccgagcg ggacgctttc tccgtcgtca ggatcacggt
      121 cggcaaggag aacacagccg aggatgtcga aggtttcctc gcggccttct cgaccgcggt
      181 cgcccaactt cgagagctct ccccgctcta cgcgggctga aggctggata ttttgtcgga
      241 tcgctacaca aaacaggtca tagaccatct cgtcaggccg cgcaatgcgg gagagatcct
      301 cgacccttct ggccggggag agtcgggcga cgcatcctgc ggcgacgtcg catgctttac
      361 ggtcggtatc tcggagaacg ttctcgaaga ggtccggtat aaggtctacg gctgcgccgc
      421 atgtatagcc gccggctcgg ccctggccga actggtcgaa ggcaagcatc tcctcgacgc
      481 cgcccgcgtc tcgaaagcgg acctcgagga cgccctcggc ggagcgctcc cggcgggcaa
      541 ggggcacgcg ctcacgctcg tcctcgacgc gctgcacaag gccttcgagg accactggaa
      601 cagcacggcg ggcgagatgc tcttcgacgg agagcgagga aagggaaacg ggggcaaaac
      661 cgtcgtggcc gcgatgagcg ggggcgtgga ttccgctgtg acggccctgc tcctcaaaga
      721 ggccggctac ggcgtcgcga ccgtgacgtt ccgcctgcac gacggagaga ggggcagccg
      781 ctcctgttgc tcgcctgaca cagtgctctt cgcccgcgac acggcgcaca ggatgggact
      841 gccgcacttc acgctcaacc tcaaggagct cttccagagg cgcgtgatgc aggacttcgt
      901 cggctcgtac aaggagggca ggacgccgaa tccctgcgtc gcgtgcaacg cgcacgtcaa
      961 gtttcacgct tcctcgttcc tcgctgaccg gctggggttc cattacgtcg cgacgggcca
     1021 ctacgccagg gtcgaagaag ggccgacgct cgcccgtccc gttgacgaga gcaaggacca
     1081 gacatacgtc ctgtggcctg taccgcggga tctcctgtct cgtacgctct tccctctcgg
     1141 cgagtacaga aagaccgagg tgcgcgccat cgccgaggag cgtggccttg ccgtcgcata
     1201 caccccggag agccaggata tctgcttcat ccccgacggg aactaccgta agttcgtccg
     1261 caagaaggtc gaggcggagc cgggggacgt ggtggacgcc ggggggcagg tgctcggtcg
     1321 tcacgccggc gtggtggact ttacagtcgg acagcggcgc ggcataggcg tctcggcccc
     1381 gacgccgctc tacgtgaccg aggtgcgtcc tcgttccggt caggtcgtcg tcggtcgcag
     1441 gcaggacctc gaagtccagg aggtcagggt cagggacctc aactggttcc tcgatccgcg
     1501 ggaagcccgc ttcgtccagg tccgctacaa cggatcggct gtcccttgca cggtcgagga
     1561 ggcggcggat ggtgattgga tagcccatct cgatgaggcc gtaatgggtg tcgcgccggg
     1621 ccagtcggcg gtcttctaca ccggagacgg acagcgggtc gtaggaggcg gcgtcgtggc
     1681 gcgccggggc tactagaagc agtgtatctg gcgtgcaatc atagctataa tccgtgttct
     1741 cagggaccct ggacggaggt acggtttgga tattttcgta gagctgatga aaggattgct
     1801 cttcgcgggg atagccgtcg cattcttcgt cctcgcgtgg atgttcttga gattggcgag
     1861 gatcttctcg accaccgagc agacgatcaa agacgtctcc gggcaggtcg ttccgttgct
     1921 cgggaagacg cacgtcaccg tggacaacat aaacagccag ctctcgcagc tcgacgatgc
     1981 cgtaggcgac gtggccggta tgacgggtga gctcgaccag acgacagccg tcatcacgcg
     2041 cggcgtcagg ggaggtgtga tcaaggtctc ctcagcgacg gccggtatct cgcgcgggat
     2101 aagcaccttc ctcggtggcg agaagcgcgg ggaggaggag tagtggcgag ttggggcagg
     2161 gtcgtcgcag gtgtcgtgat cggggtcgcc ggtacgattt acgcgaccaa cgaggacgtt
     2221 cgcaagaacc tgccgaaggc cgcgcgagac ctgccggata acgtcaggcg ccgctacaag
     2281 agcgccgtgt ctgccgcgcg cgaggcttcg gcgcggcgtc gggaggagat cctgcgcgac
     2341 ctcgaaagcc acggcggcgg tcaccacacc gcgcgcggtg cgccccccga ccctgacgcg
     2401 gctactgtga cgcaggagga agccgcatca ggcgaagaga cgacggaagt gccagagcaa
     2461 cgctaaggaa ggaattagct tgaccgaaga tcactccaag gagtacgctt acctcagact
     2521 cccgccgcac gaggagctgc gatcctgcgt gggtctcgtg cttgcgggga tggcggcacg
     2581 ggccagggta ggagtggggg ggttggagga ggccgtggag gtactggagg gcttccacgc
     2641 tggcgacgct ccaacccgct tccgcttctc cctggccggc gaaggagtgc tcgccgaggt
     2701 cgaggagcga gccgccgacg ggtctgggcc ccgctggcgc accgtcgtag agctgctgtc
     2761 gtgagccggc gctacaggag gccggacaaa gtgcgcacgc ggcggctgct ggtccgttac
     2821 cacaagcacg gcgacaagaa cgcccgcgag aaggtcatac aggaacaact tcccctcgct
     2881 gagttcctcg cccgcaaatt ctcaggtcgt ggggagcctc tggacgacct ggtccaggtg
     2941 gcctcagtcg gcctgatcaa ggcggtcgac cgcttcgacg ttgaccgcaa gatcgagttc
     3001 tcgacctacg cgacgccgaa tattctgggt gagatcaaac gctacttccg cgacaagggc
     3061 tgggcgatgc gcgtccccag gggcctgcaa gagctgcgcc agtcggcgaa ggagaccatc
     3121 cgcaacgaga ccgtgaggac gggacaatcg cccacgctgc atgacctggc cgaatccctc
     3181 gacgccgacg tcgagagcgt cgcggaggct ctgacgctag ggagggcata caacactgcc
     3241 agcctcgacg cccccgtgag ccaggacgag cccgacgggg acacgatcat ggacctgcag
     3301 gccgacgaca actctcctat tgagggcctt gaggacagaa tcctgctcca ggaggcgatc
     3361 gagggcctaa ggggccagca gcagcagatc ctgaagctcc gatttaacga gggcaagacc
     3421 cagaccgaga tcgccgacca cataggcgta agccagatgc acgtctcccg ccttctcagg
     3481 cgcgctcttg aggacctgcg ggttgaactg gagcagttgg aacgtgaccg cgagacggaa
     3541 actctatcca gggatggggc gcatgcagag tagcgagata cggcgccggt tcctggactt
     3601 ctttatcgag agggaccacc gcttctaccc tagctcgtcc ctgatccctc acaacgaccc
     3661 gacggtcctg ctgaccaccg cggggatgca gcagttcatc acctacttta cgggccagga
     3721 ccggcccccg acaccccggg ccgcgagcgc ccagaagtgc ttccgcaccc aggacatcga
     3781 agaggtcggc gacccgagcc acctgacgtt cttcgagatg ctcggcaact tctccttcgg
     3841 cgactacttc aagaaagagg cgatcgagtg ggcctgggag ttcctgacgg aagagcttgg
     3901 gattccgcca gagcgactct ggatcacgat cttcgaaggc aacgaggacg caccagaaga
     3961 cctcgaagcg aaggaactat ggatgggcgt cggtgtaccc gagcacaaga tcttcggcct
     4021 ccccaagagc gagaactggt ggggcccgcc cggagactcg ggaccgtgcg gaccgtgctc
     4081 ggagatctac tacgactacg gcgaggagta tgccaaggga gacccctccg aggaccccag
     4141 gtacggacct ggcggggacg agggagatcc gcgcttcctg gagatctgga acctcgtctt
     4201 caaccagtac gagcagcgca aggacggaag cctggtcccg ctggccaaga ccggaataga
     4261 tacgggcctc ggcctcgaac ggacaacggc agccgtgcag gacgtcctct acgtctacga
     4321 gaccgacgtc tacgcgccga tactggagaa gataagggag tacaagggcg taactcttgg
     4381 cgaatccgaa gctacagacc gcgcgcttta catcgtcgcc gatcacgcgc gcggtatagc
     4441 cttcctcatc gcggacggcg tccgtcctgg gaaccagcgc cgcgaatacg tccttagacg
     4501 catgatacgc cgggcgatgt tgcaggcata tacgcagctt gggatgacgc cggagcaact
     4561 cggaggggtc gctgaagtcg tcgttgacta cataggcgac ttttacgagg agttacgtgt
     4621 tgcgcgggag gacatacgcc gcgtcgtaac tggcgaggct gcgaggttcg tggagatcta
     4681 cgagtcgggg atgtcgctgc tgaacgcgga gatagagcgt gttcagggtg aaggcttccc
     4741 tggtgacgtg gccttcacgt tgcacgacac gtacggcttc cccgtggagg ttacgcggga
     4801 ggtacttgct gagagggggc tctcgctcga cgaggccggc ttcgaggcgg cgatggacgc
     4861 gcagcgagag cgcgccaggg atgccatgca gggctacgac agggtggttg ctgctttcag
     4921 ggaccaggag ataaggagcc gcttcgtcgg ctacgagcgc gagcaggtgg agacgaggat
     4981 agtcgcgatc gagcccgtgc ccgacgccga gggagaagtc tacgttgtcg tggcagagaa
     5041 cccgttctat gcgacgggtg gagggcagat cgctgatgag ggttggatca cctctgaaga
     5101 cggacaactc gaggtcttcg attcgatccc tgctggcgac tatcaggtac ttagagccag
     5161 ggtcgagagg ggttcgttcg aggccggtga tgctgtaacg gcgtccataa accgggtgcg
     5221 gcgccagcag atagaggcca accacacggc gacgcacatc ctgcactggg ccttgagggc
     5281 tgtcctcggc caggacgtcg tgcaggcggg gagctacgtc ggccctgaca ggttgcgatt
     5341 cgattaccga tactcaggga aagtcactga agatgagatc gggcgcgtgc aggagttgtc
     5401 cctgctcaag atcaccgaga accagccggt caggtactac accaccacgc tcgaggaggc
     5461 tcgcaacctc ggcgcgatca tgctcttcgg cgagaagtac ggggatctgg tccgggtcgt
     5521 cgagatagac ggcttctcgc gcgagctatg cggcgggacg cacgtgcgag ggaccgctga
     5581 gataggggcg ttcaagatac tctccaacag gaagcacggc gccgacctct accgcatcga
     5641 ggtcataacg ggccgagaag cactctacta cctgaccggt gcgaccgaga gagcagagga
     5701 aatctccgag gcactcaagg tcgagatcga cgagcttccc gaagcggtcg gcaggctgcg
     5761 cgaagaggtg cgaacggccc gcgaagcggc cagggagcag accctcaagc agggcctcga
     5821 agaggtgggc gacctcgtcg aaggcgccca gcaggtaaac gggacgaggg tcgtcacggg
     5881 ccaggtcgtc gccgccgacg tcagggggct gcgccagatc tccgacgacg tgaagaaccg
     5941 tctgggaggg ccatcggtcg tggtcctcgc ggcggcgctg gagggaaaag ccgtcctcgt
     6001 cgccaacctc catcccgagg tctcgcaaag agtagaggca ggtgagatag tgcgcgaggt
     6061 ctccggcgtc ctcggcggtg gcggtggcgg tggggcgacc atggcccagg ccggcggcgg
     6121 caatctcgat gctatccccg acgccctcgc gaaggtgcgc gagatcctcg accggcgact
     6181 cgcggagtag gttctgccgc aacccgatcc cgcgcaaggc cgaatagccg ccctcgacct
     6241 cggagaggtc cgtaccgggg tggccgtctc ggacccagga cgtattctgg ccactccgct
     6301 tgaggtgata ccgagcgtgg atctcgcagc ttacctccac cgacttttcg atgaagaagg
     6361 tatctccgag gtcgttgtcg gggtgccgag gacactcggc ggtgagatcg gctcccaggc
     6421 tcgcagcgtt ttggatacac tagaaagcct tagaacagag ttccccggag ttcggttcgt
     6481 ggagtgggat gaacggttca ccacccgtat ggcgtccgcc gggaggggcg gcaataagaa
     6541 gggggctgtg agggtggatc acctggccgc agcccggatg ctgcaggagt acctcgaggc
     6601 taggggcaga ctttgaggcg ccatgacaaa agtaaacgac gagaccggga ggaggtcgaa
     6661 gccaggctcg acgctctcga tctctccgac tctagaaggt cccgccgtaa acgccgctcg
     6721 aacgttggtc cgacggccct cggcgtcctg ctgctaattg ccgtccttgg atccgtctac
     6781 ctcatatact ccgccgccac tggaggccag aacgggcctg accaggccgg ggtagccaga
     6841 gtagaggtcg agaaaggcga taccctctcg gacgtggcca acaaactcga agaagccggg
     6901 gtaatcaaga atgctttcgt cttcaagctg caggccaaat atgaggggta cgggacggag
     6961 attaaaaccg gacgctacac cttcgagcag ggccaggaca gcgaggtgat cctgcagaaa
     7021 ctgacagccg gccgggccgc gcccatgata gccttcacga tacccgaggg tcttacccta
     7081 gaggagacgg ccaggaccgt cgccgcggac agccatgttc cagcgtccag gttcgaggag
     7141 gccgcgagga ggactgacta cgggtacgcc ttcctcgaca atcctgacat caagaccgcg
     7201 gaagggtatc tgtttcctgc cagatacgag ttcgaggagg gcgttaccgc aaggcagata
     7261 gtggatcggc tcctcgagca gtatctactc gaaacccagg acctcgacat cgcggaggcc
     7321 gaagagcgac taggcatttc ggaacatcaa ttagtaaccg tagcgtctct catagagaag
     7381 gaggccgcca cccccaagga gaaacccatc atagcgtcgg tgatctacaa ccggatggac
     7441 aagggtacgc cgctccagat cgacgccacg atccagtacg cactcgaaga gcccaaggag
     7501 aacctctcgc tcgccgacct taaggttgac tcgctttaca atacctacga gcacaaggga
     7561 ctgccgccag gaccgatatg cagcccgagc cgcgagtccc tccaggcagc cctcaacccg
     7621 aaagagacga actacctcta ctacgttctc agggcagacg gccagagcca cttcttcaca
     7681 agcaactacg acgatttcct cagggcgaag gaggcagcgg gacgctagca cgacggagtt
     7741 agaaaccgca ctcaagtttc aggctagtac gccgataacc cgtgtgccgg tagtgaacat
     7801 cgggattcgg gatgagcctg agcagagaga cacgatcgtc gtgcgggccg caagtaggac
     7861 acaactctgg cccagaagcg caccgcgtca cgtcgacatc cacgctcgtg ttacgggtgt
     7921 acgccactac cacggcccgt aaacaagcaa ttcatctgac ttcagacgat gcttcctcgt
     7981 aacaatggat cggtgcctgc tgcccaacga ggggtcgggg agatcctcgt ctcggagggt
     8041 gtgatcaccg gccatcagct cgaggaagca cgtcggctcg gaagtcagca agacgtgggg
     8101 aggatgctcc tcaccctcgg ctacgtcgac caggctgacc tcgcgagggc gctggcccgc
     8161 aggttgcgac tcgagttcgt cgatttgagc gtgaaggacg tagaccccgg ggcggttggc
     8221 ctggtggaaa ggaaggtcct gcgccggtat gggatgctgc cgcttcgcgt cgaaggcgga
     8281 cgactcgtcg tcgcgatgaa agaccctacc aacctctacg cgctggagga cctgagtatg
     8341 ctctcgggat atcccgtgag tccggtggtc gtggccgaga gtgacctgcg gcgggccttt
     8401 ggcgaactat tcggcatggg tgacgaggtc gcggaacttc tggatgaggc aggcagcggc
     8461 cagccggcgg aagatagcca agagctggaa ctgcgggagg atcagggtgc ctccggagct
     8521 cctaccgtga ggctggttgg gacgatcctg cgtcgtgcgg ccggtgatgg cgcctcggat
     8581 atccacatcg aaccccgcag cggggacctg gcggtacgtt tcagggtgga cggggtgttg
     8641 agggaagtca tgagcgttcc acccgctctg cggagtggcg tcatcgcccg cctgaaggtc
     8701 ctcgccagcc tcgacatagc cgaacgccgt ctgccgcagg acggccgttt ctcagtgaag
     8761 ctggacgaca ggaatatgga cctcagggtg gcaaccctgc cgacaacctt cggggagaag
     8821 gtagtgcttc ggctgctcga caatgcgaac ctccacgccg accttcgacg tctcgggttc
     8881 tcaagcaaga tgcttcaggg atacgaggcc gtattcagga gaccctacgg gacgattctc
     8941 gtaacaggac ctacgggcag cggcaagtcc acgaccctct acgccacact gggagagatc
     9001 aatgctcccg agaagaacat catcaccgtt gaggaccccg tcgagtatag gatgcgcggt
     9061 atcaatcagg tccaggtcaa ccccaggatc gggctgacct tcccttctgg tctcaggagc
     9121 atcctgcgct ctgaccctga cgtcgtgatg atcggggaga taagggaccg tgagaccgcc
     9181 aggaccagca tcgaggccgc gctcacgggc catctcgtac tggccacgct gcacaccaac
     9241 gacgcccctg ccgcggtcac gcgcttgaac gatatgggag tc
//
LOCUS       DATAIR010000023         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_185173, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000023 DATAIR010000000
VERSION     DATAIR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185173"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VHM69_00655"
     CDS             <1..1320
                     /locus_tag="VHM69_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2738926.1"
                     /translation="VAFGLAMKRYHLFNADLLINRTLVYAALTACVVGIYVFVVGYLG
                     ALLHTEDNLLISLVATGLVAVLFQPLRYRLQRTINRLMYGERDDPYAALSRLGQHLEA
                     TIAPDAVLSTVVETVAEALKAPYAAIEIERNGAYESVAATGEPVEAPLRLPLIYGGGR
                     VGWLVLGRRTGEAEFTPADRRLLDDLARQVGVAVHATRLTEDALRLSDELQKSREQLV
                     TAREEERRRLRRDLHDGLGPQLASLTMKAEAARDLLEMNPERSGALMEEITAQTQEAV
                     ADVRRLVYGLRPPALDELGLLGALRTQAAHGDRNGLRVDVEAPDRLQPLPAAVEVAAY
                     RIVQEAVTNAARHADASNCAIRITPDEAAEALRVEVVDDGRGISEDHSAGVGLYSMRE
                     RAEELGGSCKVEAVPSGGTSVRAILPLTRGAQDARERQAVEPEVRKT"
     gene            1323..1979
                     /locus_tag="VHM69_00660"
     CDS             1323..1979
                     /locus_tag="VHM69_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2738927.1"
                     /translation="MELTRVLIADDHPHFRDGLRALMLSAPDVEVVGEARDGEEAVKL
                     AERLQPDVILMDLNMPGVGGIEATRRILHTSPHISVLVISMYEDDDSVFAALKAGARG
                     YLLKGALKAEILRAIRSVMSGEAIFGPAIARRLMQYFANPGPAASKDAFPELTEREHE
                     ILELIARHETNPEIAKKLRLSQKTVRNHVSNIFTKLQVADRAQAIIRAREAGLGRKKA
                     "
     gene            complement(2025..>2413)
                     /locus_tag="VHM69_00665"
     CDS             complement(2025..>2413)
                     /locus_tag="VHM69_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2738928.1"
                     /translation="VGGSRQGERGFFYSPTVVSGLRQDDEASQNEVFGPVISVQSFSD
                     EDEAVRWANGVKYGLASSVFTKDHGRAMRVSRRLDFGCVWINTHIPLVAEMPHGGFKH
                     SGHGKDLSMYGLEDYTRIKHVMTSLG"
BASE COUNT          444 a          771 c          796 g          402 t
ORIGIN      
        1 gtggcctttg gccttgcaat gaaacgctac catctcttca acgcggacct cctcataaac
       61 cgcaccctcg tctacgcggc gcttaccgcc tgcgttgtcg ggatctacgt cttcgtggtc
      121 ggctacctcg gagcgctgct ccatacggaa gacaacctct tgatctcact cgtggcgacc
      181 ggcctggtgg ccgtgctctt tcaacccctg cgctatcgtt tacagagaac cataaaccgc
      241 ctgatgtacg gtgagcgcga cgacccctac gcggcgctct cgcggctggg gcaacacctg
      301 gaggccacga tcgcccccga cgccgtgcta tccaccgtcg tcgaaacggt cgccgaggcc
      361 ctaaaggcgc cgtacgcggc catagagatc gaacggaacg gcgcctacga gagcgtggcg
      421 gccacgggcg agccggtgga agctcctctg cgcctgccgc taatctacgg cggcgggcga
      481 gtggggtggt tggtcctcgg acggcgtacc ggcgaggcgg agttcacgcc cgcggaccgg
      541 cggttgctcg atgaccttgc ccggcaggtc ggggtagcgg tgcatgccac tcgtctgacc
      601 gaagatgctc tcaggctctc agatgaactg cagaagtcgc gcgagcaact cgtaacagcc
      661 cgggaggagg agcgccggcg tctgagacgc gatctgcacg atgggctcgg accgcagctc
      721 gcaagcctta cgatgaaggc cgaggcggcg cgggaccttc tggagatgaa cccggagcgt
      781 tccggcgctc tgatggagga gatcacggcc cagacccagg aagctgtggc tgacgtgaga
      841 cgcctggtct acgggctccg tccgcccgcg ctcgacgagc ttggtctgct cggggctctc
      901 agaacccagg ccgcgcacgg tgatcgcaac ggcctgcgcg tggacgtgga ggctcctgat
      961 agattgcagc ccctgcccgc cgcggtggag gtcgccgcct accgcatcgt ccaggaggcc
     1021 gtcaccaacg ccgcccgcca cgctgatgcc agcaactgcg cgatccgtat cacgccggac
     1081 gaggctgcgg aggcgttgcg cgtggaggtc gttgacgatg gtcgcggtat ctccgaggac
     1141 cactccgcgg gtgtgggact ttattcgatg cgcgagcggg ccgaggaact cggcggttcc
     1201 tgcaaggtag aggccgtgcc ttccggggga accagcgttc gggcaatact gccgcttacg
     1261 cgaggcgcgc aagatgcacg ggagcggcag gctgtagagc cggaggtgag gaagacttga
     1321 gcgtggagtt gaccagagtt ctcatagccg acgaccatcc gcacttccgg gatgggctgc
     1381 gcgcactgat gctctccgcc ccggacgtgg aggtggtagg agaggctaga gacggggagg
     1441 aggcggtaaa gctcgcggag aggttgcagc cggacgtgat cctgatggac ctcaacatgc
     1501 cgggcgtggg cggcatagag gccacccgcc gcatcctgca caccagcccc cacataagcg
     1561 tcctcgtcat aagcatgtac gaggatgacg attcggtctt cgcggcgctc aaggcgggcg
     1621 ctcgcggata cctgctaaag ggcgctctga aggccgagat cctacgtgcc ataaggtccg
     1681 tcatgagcgg cgaggccatc ttcggacctg ctatcgcccg acgcctgatg cagtatttcg
     1741 ccaatcctgg tcccgccgcg tcgaaggatg ccttccccga gctcacagag cgtgagcacg
     1801 agatactgga gctcatcgcc cgccacgaga cgaaccccga gatcgcgaaa aagctgcgac
     1861 tgagccagaa gaccgtccgc aaccacgtct caaacatctt taccaagctc caggtcgcgg
     1921 accgcgccca ggcaataatc cgcgccaggg aagcgggtct ggggaggaaa aaggcgtaga
     1981 cgctccggga gcgcctacgc cggacggcga atctgaacct atacctaacc caggctcgtc
     2041 atgacgtgct tgatgcgggt gtagtcctcc aagccgtaca tggagagatc cttgccgtgg
     2101 ccggagtgct tgaagccgcc gtggggcatc tcggcgacga gtgggatgtg cgtgttgatc
     2161 cagacgcacc cgaagtccag ccgccggctg acccgcattg cccgtccgtg gtccttcgtg
     2221 aacacacttg atgcgagccc gtatttcact ccgttggccc accgcaccgc ctcgtcctcg
     2281 tcggagaagc tctgcaccga gatgacgggt ccgaagacct cgttctgcga agcctcgtcg
     2341 tcctggcgca gcccggagac gacggttggg gagtagaaga agccccgctc gccctgacgg
     2401 ctgccgccga ccg
//
LOCUS       DATAIR010000024         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416595, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000024 DATAIR010000000
VERSION     DATAIR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416595"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="modA"
                     /locus_tag="VHM69_00670"
     CDS             <1..511
                     /gene="modA"
                     /locus_tag="VHM69_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2738929.1"
                     /translation="KQGLVSGKPRVFVKNREVVMVPKANPANIRSFRDVATPGTRLVL
                     AEDAVPAADYAMEILGKADEEYGAGFKKDVLSNVVSREADVRASVNRVTVGDADATFG
                     YSSDYTPDIRDKVEVVPIPADLNIVATYPIAALEDAKSPTLAKKWVDLVTSEEGQRVL
                     KQWGFEPAA"
     gene            508..1338
                     /locus_tag="VHM69_00675"
     CDS             508..1338
                     /locus_tag="VHM69_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2738930.1"
                     /translation="MKGRNKGVLSQRVPRGSDAVASGLAVAALALLAGFISLPLLSLV
                     VWTIDEQAWRAMASPTAVDALLLSIRTTAITMLILLVVGTPAAYVLARVNFRGNRVIN
                     TLIDIPVVLPPSAAGIALLLAFGRFGLVGQHLEVLGITLTFTTAAVVMAEIFVAAPFY
                     VRQAATGFGAVDLTVEEAALVDGAGRSTVFFRITVPLAFPALVAGALTAWARALGEFG
                     ATVIFAGSFRGVTQTIPLAIFAEFHRDLDAAVALSVLVLGFAFAVILAVRYLTNRKNY
                     "
     gene            1705..>4865
                     /locus_tag="VHM69_00680"
     CDS             1705..>4865
                     /locus_tag="VHM69_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HEX2738931.1"
                     /translation="MNDNGADVRAAIETSRKELLDLGLRNPLLNYRPSRARGVQITDG
                     SPEEVFRILVTESKTMTFKGVPEEDAEDGEELAEYLDQPEESASGEAARHLDRKLQTS
                     LTSPKLQARLLKTQRAARTFIEEQGINTLFLALGMLRWFEDENSQVERSAPLVLVPLH
                     MERSSVQERFHVEYTGDELGENLSLATKLRLEFDISLPEMPGADEMDIPYYFDEIERA
                     VAREGRWRVDRRAISLDFFSFGKFLMYRDLEEERWPENGRLSRHPVIGRLLGEPEGSD
                     GSAAPFIADDENVDKHLAPAQVRHVVDADSSQTLALLDANNGRNLVIQGPPGTGKSQT
                     ITNLIAEAVSHGKKVLFVSEKMAALEVVKRRLDSVGVGEACLELHSRKTKKTQILQEL
                     GRTLSLGSPRLQGTEDDLGVLEDLRDRLNDYSEAVNEEIGESGVTPYKAVGELISQGE
                     PGANAPRLDFDAMSGWNVSEFRRKQLLVERLQSKLSEMGSPRDNAFNGTTLTVLLPTE
                     ARRIREKISSGLDATKRLRDASRELSEALKLPTPATRDDCEALLLAAKSVEDAPDLSG
                     INLKAEEWLTSRKAIEELVSAGLTHSRIVNEISDVLIPEAWDQDLLEMRQVFAAHESW
                     LRRLFSSQFKAERDRLRGLCRESLTESHEEQLALIDAVLEARRCRNVVEEYERLGERV
                     LGERWEGSRSDWERISGVVTWVQELHDNVERGAVPREILDCLDEGANAQDVSALRAAL
                     EEVLKEHARALSDAVEELSFGGEQVARSLESWTFDEQEAVLGGWVERFHELQEIVSFN
                     QLARELREQGLSSAVEVGESWEGAGSGLVDAYRYTWFEGLAERALRERTVLSNFDRGG
                     HEQIVARFRELDALNLRRNRALVALSHWKGVPNHEGGGQLAVLRRELNKKRRHLPIRQ
                     LMRRAGNAVQAIKPVFMMSPLSIANYIEPGSVEFDLVVFDEASQVRPVEALGAVARGR
                     QTVVVGDDRQLPPTRFFDSLLEGEPEDELNVTGDLESVLGLFLAQGMPQRMLRWHYRS
                     RHESLITVSNHEFYDDRLTIFPSP"
BASE COUNT          946 a         1477 c         1604 g          838 t
ORIGIN      
        1 gaagcaaggc ctcgtctcgg ggaagccgcg ggtcttcgtc aagaaccgcg aggtcgtcat
       61 ggttcccaag gcaaacccgg cgaacatacg gagtttccgg gatgtcgcga cgcctggcac
      121 caggctcgtg ctggcggagg atgcggtacc ggccgccgac tacgccatgg agatcctggg
      181 taaggcggac gaggagtatg gggccggctt caagaaggac gtcctctcca atgtggtctc
      241 ccgcgaggcc gacgtaaggg catcggtcaa ccgcgtgacc gtgggggacg ccgacgcaac
      301 cttcggctac tcaagcgact atacgccgga cataagggac aaagtagagg tcgtcccgat
      361 cccagccgac ctgaacatcg tcgcgaccta ccccatagcg gccctcgaag acgcgaagag
      421 cccgactctg gcgaagaagt gggtggatct cgtgacgagc gaggagggac agcgcgtgct
      481 gaagcagtgg ggcttcgagc ccgcggcgtg aaaggtcgga acaaaggagt cctgtctcaa
      541 cgggttcccc gcgggagtga tgctgtcgcc tccgggctgg ccgtggctgc gctcgcgctg
      601 ctggccggat tcataagcct gcctctactg agcctggttg tctggacgat agacgagcaa
      661 gcctggcggg caatggcctc cccgactgcg gtagacgccc tgctcttaag catccgcact
      721 actgccataa cgatgctgat tctgttggtg gtgggcacgc ctgcggccta cgtcctggcg
      781 cgcgtgaact tccgggggaa ccgtgtgatc aatacgctca tagacatccc cgtggtgctg
      841 cctccgagcg cggccggaat cgcgctgctc ctggctttcg ggagattcgg cctcgtgggg
      901 caacacctcg aagttctcgg cataacgctc acattcacga ccgctgcggt cgtgatggcg
      961 gagatcttcg tggcggctcc cttctatgtg aggcaggcgg cgacgggttt cggcgcggtt
     1021 gacctgacgg tggaggaggc tgccctcgtt gacggggcgg gccggtccac cgtgttcttc
     1081 aggatcacgg ttccattggc gttcccagcg ctcgtcgccg gggcgctcac ggcttgggcg
     1141 cgcgcccttg gagagttcgg cgccacggta atattcgccg gcagtttcag gggcgtcacc
     1201 cagaccatcc cacttgcgat cttcgccgag ttccacagag acctcgacgc cgccgtggcg
     1261 ctctccgtcc tcgtgctcgg cttcgcgttc gccgtgatcc tggccgtcag gtacctcacg
     1321 aaccgtaaga attactaggc taggattccg cgatcggatc tccgaagaag gcgaaaaggg
     1381 gcgccttcct ttgaggaagc gttgtgttag ctatcatccc ttcggcaagc actcggtctg
     1441 cttcgcaggc gtgccgcttc atcaactcat cgaagacttc aatagctctg tcacgtagct
     1501 cctctccagt ggtcggggct ccgtaaatag aatacaattc ttcaccgtca actagaccag
     1561 cgcgttgtgg acttccacgg gcggatagat tctgaacacc gcgcggatgt agaatacttc
     1621 catcatctta cgatggcggg caatcaatgc cccgtcagtg gggatgtttt gtgggttgca
     1681 tatagcggtg gggcggggga atacgtgaac gataacggtg cagatgttcg cgcggccata
     1741 gagacaagca ggaaggaact gctggatctc gggctaagaa accccctgct gaactaccgc
     1801 ccctcccgcg ccaggggcgt ccagatcacg gacggatctc ccgaagaggt cttccgtatt
     1861 ctcgtaacgg agagcaagac catgacgttc aaaggcgtgc cggaggaaga cgcggaggac
     1921 ggcgaagagc tcgcggaata cctcgaccag ccagaagaga gcgcttccgg cgaagccgcc
     1981 cggcatctcg accgtaagct gcagacctcc ctgacctccc cgaagctgca ggccaggctc
     2041 ctgaagacgc agagggcggc acgtaccttt atcgaggagc aaggcatcaa caccctgttt
     2101 ctcgcgcttg gcatgctccg ctggttcgag gacgagaaca gccaggtgga gcgctcggcc
     2161 cccctcgtgc tggttccgct ccacatggag cgttcgagcg tccaggagag gttccacgtc
     2221 gagtacacgg gcgacgaact cggagagaac ctttctctgg cgaccaagct caggctcgag
     2281 ttcgacatct ccctgcccga gatgccaggc gcggacgaaa tggacatacc ctactacttc
     2341 gacgagatcg agcgtgccgt ggcccgggag ggtcgctgga gggtagaccg gagggcgata
     2401 tcactcgact tcttctcgtt cggaaagttt ctgatgtatc gcgacctgga ggaggagcgc
     2461 tggcccgaga acgggcggct ctcccgccac cccgtcatcg gcaggctgct cggagagcca
     2521 gagggttccg atgggtccgc cgcgcccttc atagcggacg acgagaatgt ggacaagcac
     2581 ctcgctcctg cgcaggtgcg ccacgtggtg gacgccgaca gctcccagac tctcgcgctg
     2641 ctcgacgcga acaacggacg caacctggtg atccagggtc cccccggcac gggcaaatcc
     2701 cagaccatca ccaacttgat cgcggaagcc gtgtcgcacg gcaagaaggt gctcttcgtc
     2761 tccgagaaga tggcggcgct cgaggtggtg aaacgccgcc tcgacagcgt cggagtcggt
     2821 gaggcttgcc tggagctaca cagccggaag acgaagaaga cgcagatcct ccaggaactg
     2881 ggcaggacgc tctcgctcgg cagcccccgc ctccagggca cggaagacga tctgggcgtc
     2941 ctcgaagacc tcagggatcg cctcaacgac tactccgagg cggtcaacga ggagattgga
     3001 gagtctgggg taacacccta caaggccgtc ggcgagctga tcagccaggg cgagccgggc
     3061 gcgaacgcac cgcgcctgga cttcgacgcc atgagcggct ggaacgtctc ggagttcagg
     3121 cggaagcaac ttctcgtcga gcgcctccag agcaagctgt cggagatggg ttcgccccgc
     3181 gataacgcct ttaacggaac caccctcacc gtgctgctgc cgacagaggc caggcgcatt
     3241 agggagaaaa tatcctcggg cctggatgcc acgaagcgcc ttcgagatgc gtcccgggaa
     3301 ctctccgagg cactgaagct cccgaccccc gcgacgagag atgattgcga ggcgctcttg
     3361 ctcgcggcca agagcgtcga agacgccccc gacttatccg gcattaacct gaaggcggag
     3421 gagtggctga ccagccggaa ggcaatcgag gaactcgtgt ccgccgggct cacccactcg
     3481 cgcatcgtca acgagatctc ggacgtcctg ataccggagg cttgggacca ggaccttttg
     3541 gagatgaggc aggtgttcgc ggcccacgag agctggttgc gcaggctgtt ctccagccag
     3601 ttcaaagccg aacgcgacag gctccggggg ctgtgccgcg agtccctaac ggagagccac
     3661 gaggaacagc tggccttgat agacgccgtt ctcgaagccc gccgttgcag gaacgtcgtg
     3721 gaggagtacg agaggctcgg agagcgggtg cttggcgaga ggtgggaggg ctcacgctcg
     3781 gactgggaac ggatatccgg ggtggttacc tgggtgcagg aactgcacga caacgtggag
     3841 cggggggccg tgccgcgaga gatcctcgac tgtctggacg agggagcgaa cgcgcaggac
     3901 gtctccgccc ttcgtgcagc cctcgaagag gtcttgaaag agcacgcccg cgccctatcg
     3961 gacgccgtcg aagagctctc gttcggggga gagcaagtcg cccgatccct cgagagttgg
     4021 actttcgacg agcaggaggc ggttctcggt ggctgggtgg aacgcttcca cgaattgcag
     4081 gagatcgtca gcttcaacca gctcgccaga gagcttcgag aacagggtct ctccagtgcc
     4141 gtcgaggtcg gagagagctg ggagggggcc gggtccggac tcgtggacgc gtaccggtac
     4201 acctggttcg agggtctggc cgagagggct ctcagggagc gtacggtgct ctccaatttt
     4261 gatcgcggag ggcacgagca gatcgtggcc aggttccgtg aactggacgc tctgaacctc
     4321 cggcgcaacc gtgcgctcgt ggccctgtcg cactggaaag gcgtgcccaa ccacgagggg
     4381 ggaggccagc tcgccgtcct caggagggag ttgaacaaga agcgacgaca cctcccgatc
     4441 cgccagctca tgagaagggc gggcaacgcc gtgcaggcga taaagcccgt cttcatgatg
     4501 agcccgctct cgatagcgaa ctatatagag ccgggaagcg tggagttcga cctcgtggtg
     4561 ttcgacgagg cgagccaggt caggcccgtc gaggcgctcg gggcggtggc ccgcgggcgg
     4621 cagaccgtcg tcgtcggcga cgacaggcag cttcccccga cgcgcttctt cgactcgctg
     4681 ctcgaaggcg agcccgaaga cgagctgaac gttaccgggg atctcgagag cgtcctcggg
     4741 ctgtttctcg cccaggggat gccccagagg atgctccgct ggcactaccg gagtcgccac
     4801 gagtccctta taacggtctc gaaccacgag ttctacgacg accgcctgac catcttcccg
     4861 agccc
//
LOCUS       DATAIR010000025         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_370480, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000025 DATAIR010000000
VERSION     DATAIR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370480"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VHM69_00685"
     CDS             complement(<1..474)
                     /locus_tag="VHM69_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2738932.1"
                     /translation="MAESFSVGVVGSGYVGLVTGACLAHIGHRVSCVDKNGALVDQLR
                     EGRMPIYEPGLEELVSRDVSRGRLSFTTDLPEVVRDSDVVFIAVDTPQGDDGSADLGS
                     VAAVARSIGGALAGISRNAPLVVVNKSTVPVGSGDYVSMLIREGAAENDGEDVDYR"
     gene            complement(562..1938)
                     /locus_tag="VHM69_00690"
     CDS             complement(562..1938)
                     /locus_tag="VHM69_00690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2738933.1"
                     /translation="MKGTLKRLLLMGAPRRLLSIVVLVLADAVAMLAGLAAATYATEG
                     DVGRPFSLAPVLLAIWISIFAAYDLYNRARVRRNPGALVGAGLVWAGLLVVGEGLYPQ
                     SGLTAGMVLLAASLSLAVDGALRVLYEQAIELLYRRGLGKIPTIIIGDEAGRTRVRRM
                     MEQTSGAYACVAEVDLGEGAVDLSALRATLDRTGARSVVLVGGERLPDEELLDLLRSV
                     RLRGVRMRVVPGALTLMRSQPTVSQSLGLPLVEVRYPRLDNTQRLMKRALDVSVSAAG
                     LVFLSPLFLTLAVFVKSSSPGPVLFRQKRAGADEGVFVCLMFRSMYEHSEVRQPELED
                     LNEVEGAAFKIRDDPRVTPFGRFLRRWSLDELPQLVNVLKGDMSLVGPRPLPLRDFER
                     MGELHKRRLAAVPGMTGLWQISGRSNLPFEEMVRLDLFYIENWSLSFDVKIILRTLGA
                     VVRREGAY"
     gene            complement(1985..2422)
                     /locus_tag="VHM69_00695"
     CDS             complement(1985..2422)
                     /locus_tag="VHM69_00695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2738934.1"
                     /translation="MDYSLTLGDVVYSPFRSGTVTFAGQNTTHQDYGVFVYIKADNGK
                     YVNVSAHLSGLASGIEPGATVDCNTIIGFAGDTGGPNISVGQVHLHQAFYRYPDYNPD
                     GSPFGGRGLQAVYFHYIRGEDGIYELGWSHTDTQISKGDNVSY"
     gene            complement(2606..3741)
                     /locus_tag="VHM69_00700"
                     /pseudo
     CDS             complement(2606..3741)
                     /locus_tag="VHM69_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3858..>4438
                     /locus_tag="VHM69_00705"
     CDS             3858..>4438
                     /locus_tag="VHM69_00705"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2738935.1"
                     /translation="MGLGELLQAHRGGEDELFYPAYVQDALGVSRSGYYDWRGRTPSN
                     RTRQDDALTETIRHIHERSRRTYGSPRVHAELRALGSRCGRKRVERLMRGAGLRGCMR
                     GKRIATTHRSKRAAPADDLVKRGFRATKIDRIWVADITYVATDEGFLYLAFILDVYSR
                     RIVGWAMENHLRTEIVVDALQMAIWRRKPAPGLV"
BASE COUNT          833 a         1448 c         1352 g          805 t
ORIGIN      
        1 gcggtaatcc acgtcttccc catcgttctc ggcggcgccc tccctgatga gcatggagac
       61 atagtcaccc gaaccgacgg gtacagtgct cttgttcacc acgacgagcg gggcattcct
      121 gctgatcccg gccagcgcgc cgcctatgct gcgcgcgacg gcggcgacgc tcccgaggtc
      181 ggcggaaccg tcatcaccct gcggggtgtc cacggctatg aagaccacat cagagtcgcg
      241 cacgacctca gggagatcgg tggtaaagga gagccgtcca cggctcacat cacgagagac
      301 cagttcttcg aggcccggct cgtagatcgg catcctgcct tcccgcagtt gatccacgag
      361 cgccccgttc ttgtccacgc acgagaccct gtgtcctatg tgcgccaggc aggcgcccgt
      421 cacgagccca acatacccgg acccaaccac tccgacgcta aaactctcgg ccaaaacttc
      481 tcctcgaaca tcctgcaaga tccggcaaaa cttacactct cggcccccga cttacaagga
      541 aagcgccggg aaacacccct cttagtacgc gccttcgcgg cgcacgacgg cgccaagggt
      601 gcggagtatg atcttcacgt cgaaggagag cgaccagttc tctatgtaga agagatccag
      661 gcgcaccatc tcctcgaacg ggaggttgct gcgaccgctg atctgccaca aaccggtcat
      721 cccaggcacg gccgcgagcc tccgtttgtg tagttctccc atccgctcga aatcgcgcag
      781 tgggagcgga cgcggcccca ccaggctcat gtcacccttc aggacgttca ctagttgcgg
      841 caactcgtcg aggctccatc gccgcagaaa ccgcccgaac ggggtcacgc gggggtcgtc
      901 cctgatcttg aacgccgcgc cttcgacttc gttcagatct tcaagttcgg gctggcgtac
      961 ctcggaatgc tcgtacatcg agcggaacat caggcagacg aagaccccct cgtcagcccc
     1021 ggcccgcttc tggcggaaca gaaccgggcc cggggaagac gatttcacga acacggcaag
     1081 cgtcaggaag agcggggaga ggaagaccag cccggccgcc gacaccgaca cgtccagggc
     1141 gcgtttcatg agccgctggg tgttgtccag gcggggatac cggacctcca cgagcggcag
     1201 cccgaggctc tgggagacgg tcggctggct ccgcatcagg gtgagcgcgc ctggaaccac
     1261 cctcatccgc acgccccgca ggcgcaccga gcggaggaga tcgagcaact cctcgtccgg
     1321 cagcctctcg ccaccgacca ggacgacgct cctcgcgccc gtacggtcca gagtggcccg
     1381 cagcgcggag agatccaccg cgccctctcc cagatcaact tcggccacgc aggcgtatgc
     1441 acccgaggtc tgctccatca tcctgcgtac cctggtcctc cccgcctcgt ccccgatgat
     1501 gatcgtgggt atcttcccga ggccccggcg gtagagaagc tcgatagcct gttcgtagag
     1561 taccctgagt gcgccgtcca cggcgaggga gagggaggct gcgagcaaca ccatccctgc
     1621 ggtcaggccg ctctggggat acagcccctc ccccaccacg agcagcccgg cccagacgag
     1681 ccccgccccg accagcgcgc ccggattgcg cctgaccctg gcgcggttgt atagatcgta
     1741 ggccgcgaag atggagatcc agatcgccag cagcacgggc gccagggaga atggccgtcc
     1801 gacatcgccc tccgtggcgt aagtggcggc ggcgagcccg gcaagcatcg ccaccgcgtc
     1861 agcgagcacc agaaccacaa tgctcaggag acgccgcggc gctcccatca acaacagcct
     1921 cttaagggtc cctttcaagg tattagaagt tcctttccgg gaccagtata cagtcgcggc
     1981 gccgtcagta gctgacgttg tctcccttgg atatttgggt gtcggtgtgc gaccagccca
     2041 gctcgtagat gccgtcttcg ccacggatgt agtggaagta gacagcctgc aggccgcgtc
     2101 caccgaaggg agagccgtcc gggttgtagt caggatagcg atagaaagcc tgatgcaggt
     2161 gtacctggcc aactgagata ttggggccgc cagtgtcgcc agcgaagccg atgatggtat
     2221 tgcaatccac ggtcgcgccg ggttcgatgc ccgaggcgag cccgctcaga tgcgccgaga
     2281 cgttcacgta tttgccgttg tccgccttga tgtagacgaa gacgccgtag tcctggtggg
     2341 tggtgttctg cccggcaaac gtcaccgtac cgctgcggaa cggtgaatac accacgtccc
     2401 cgagggtcag cgaataatcc acggcgaact tgtcgttggt gttgtaaggc tcatcgcagc
     2461 gaccgtatcc aggcccgagg ttcccgtggg tgtattcgcc ccagtagttg ggctggccga
     2521 ctatagagta tccggtacgg ataccgtctc cggatctacc gttggccccc gcaccgtgct
     2581 gcgtctgaaa gaagaagggg tgggttcaat cggtgagcgc aacagtcttg ctcagtgtag
     2641 cagccggggt gctgtaaccc agggtcttgc gcggtcgagt gtttagcttt agcgcaatca
     2701 ggtcgaggtc gtgctggccg tacacggata gatcggtctt cctgggcagg tactgtctca
     2761 agagcctgtt ggtgttctca ctccttccac gctgccaggg actcttcggg tcgcagaagt
     2821 agacggcaac gtcggtggct accgtgaact gcttgtgggc ggccatctcc gttccccggt
     2881 cccaggttag ggtgtcgatc atggtcctgg gtaggcgcct tatctgctcg ctaagggccg
     2941 ctacaacgct ttcggtgtcc ttgccgctaa cgcgcaccag catcacgaac cgcgagctgc
     3001 gttcgaccag ggtggccacg tgggtgttgc gcgatccggc gatgaggtcg ccttcccagt
     3061 gtccgggcac cgcccggtcc tcggcctcgg gaggacgctc gcggatggac acggcttctt
     3121 tgatctgccc acgcctctgg ccggccgtag aagcgtggcg ccccttacgc atcatccggc
     3181 cgctcctaag atgggcaagt agctcccttt tcagagcgcc tctggcctgc acgaacaggg
     3241 tgcggtagat cgtctcgtgt gacacgcgca tcgcctcggc gttaggatag cgtctcttga
     3301 gccaaccaga gatctgctcg ggcgaccagt cctcctttag cttgtttgct accacatcgc
     3361 gcaagagatc gttcatggcg agcaggcatc ttttcggacg gcgagctctg tggcaggcac
     3421 gttcctcggc tactgtggcc cggtagtctt gacgcccgcc gttacggtta acttcccgac
     3481 ttacggtcga tgcggaccgg cccaggcggg cggcgatggc gcgcatcgaa tctcctgacg
     3541 cgagccccga gagatctcct cccgctcggc caagctcagc atccacctcg gcctgcatcg
     3601 ctgaggcgga gcgatcccac cggtgacctt gagcatcccg tggatggagc cggggggttt
     3661 ctttaaagcc ctggagatgt cactgatgga ctctccggcc ttccatcgct cccacaactc
     3721 ctgcttgcgc gcctcggaca accctccagg acgacctagc ctcgccactc atgcacctcc
     3781 ggtccgaacg acaccgaaaa cctaccccgt gttgcaccga ccggttgaac ccaccgccgt
     3841 cttcttcgcg aaggagcatg ggactcggtg agttgctaca agctcatcga ggcggagagg
     3901 acgagctttt ctatcccgct tatgtgcagg atgctctggg cgtttccagg agcggctact
     3961 acgactggcg tggcagaacg ccctcaaaca ggacccgcca ggacgacgcc ctcaccgaaa
     4021 cgatccgcca catccacgag cgcagccgcc gaacctacgg atctcccagg gtgcatgccg
     4081 agctcagagc actcgggagt cgctgtggtc gtaaacgggt cgagaggctc atgagaggag
     4141 ccggactacg agggtgcatg cgcggtaaga ggatagcgac cacccaccgg agcaagaggg
     4201 ccgctcctgc cgatgacctc gtgaagaggg gcttccgggc cacgaagata gacaggatct
     4261 gggtggcgga catcacgtat gtcgccaccg acgaaggctt cctgtacctg gccttcatcc
     4321 tcgacgttta ttcccgcagg atcgtcgggt gggctatgga gaaccatctc aggacggaga
     4381 tcgtagtgga cgccctacag atggccatct ggagacggaa gccagctcct gggctggt
//
LOCUS       DATAIR010000026        11300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_46729, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000026 DATAIR010000000
VERSION     DATAIR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46729"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHM69_00710"
     CDS             <1..265
                     /locus_tag="VHM69_00710"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2738936.1"
                     /translation="IGGDFIEVFVDAPLEVCAERDVKGLYKKAFSGEIPQFTGVSDPY
                     EAPAAPELHIKTDEEDAQASARKVIGYLEELGYLRPDREFSRL"
     gene            904..1794
                     /locus_tag="VHM69_00715"
     CDS             904..1794
                     /locus_tag="VHM69_00715"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2738937.1"
                     /translation="MILVTGGAGFIGSHLVDTLLVTGQQLVVLDNLASGYRENLPNGI
                     KLVEMDVADARVVKIIAELKPEVVIHAAAQVSVAISMQDPHLDLAVNVQGTANVIEGA
                     KAAGARRFVFVSTGGGVYGESDGADEATLPRPKSYYSAHKYLGERYVELSGLSYANAR
                     LANVYGTRQRSDLEGGVVAIFTERLRSGQPVLINSTGEQSRDLVYVADVVDSLLTMAR
                     SDRDGTWNVGTGVSTSILELLHALEREIGPATEIRHGPPRPGDVGNSRLAIDRIENDL
                     GWRPKYDLAGGIADMIRITK"
     gene            complement(1897..3063)
                     /locus_tag="VHM69_00720"
     CDS             complement(1897..3063)
                     /locus_tag="VHM69_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX2738938.1"
                     /translation="MSKLVVLCVSITFALIVVGSVALAAVINGTAGDDKLNGTNVSDT
                     IFGFGGNDTINGLGRQDELWGGFGADTMYGQGSNDTLHGEGGNDTLAGGPGDDELRGA
                     DGVDILNGGADDDRLYDKDGNIAERDRFNCGTGNDTVRADPTDQVLASCENVNLTGED
                     LLPDLGMAQLADVQIRDTGTQKQLRFATTIVNVGAGPFEVTGRRPDTNTSEMTTTQRI
                     FDSVGDYRDRSTAATFIYSGDGHDHWHVKDLEDYDLFQLDDSGNVVEPAVVEEANKIG
                     FCFFDNANYGSSEPESYLGCEGGNPGALHVTMGLSRGWGDTYGAGTVGQYLDITGLPD
                     GRYRLRVKADGDSTDGSDRFLESDETNNSTWADLQITGDTVTVLEYGPSAPPVG"
     gene            3456..3704
                     /locus_tag="VHM69_00725"
     CDS             3456..3704
                     /locus_tag="VHM69_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738939.1"
                     /translation="MPLLLHEATPETRSRWPRAFILPVRDESGTLLETGLVLEDFTEG
                     KAVEEQPVHQAFHDPLTGLPNRALFLDRRTHALSRAKR"
     gene            3724..3900
                     /locus_tag="VHM69_00730"
     CDS             3724..3900
                     /locus_tag="VHM69_00730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2738940.1"
                     /translation="MALVYMDLDDFKRINDSLGHQTGDRLLVGLAERVGAHLRLGDTF
                     ARSEGTSSPCCSKT"
     gene            complement(3897..4022)
                     /locus_tag="VHM69_00735"
     CDS             complement(3897..4022)
                     /locus_tag="VHM69_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738941.1"
                     /translation="MEKTSWRALPSLLLQYMAMAVHPEGNIQGPFDPLGDLAHVF"
     gene            4381..7230
                     /locus_tag="VHM69_00740"
     CDS             4381..7230
                     /locus_tag="VHM69_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2738942.1"
                     /translation="MKHVANLGTPQMFRVWIVLAAVGLAGILVASLGPLEANATGTLP
                     SGFQEEEVFSGLTNPTNVEFSQDGRVFVAEKSGLIKVFDGLSDTTPTTFADLRTKIHN
                     FWDRGLLGLALAPNFPSDPHVYVLYTHDAAIGGTAPRWGTANVSSDPCPTPPGATADG
                     CVVSGRLSKLTASGDTMTGSEQVLIEDWCQQYPSHSVGDLAFGADGALYVSGGDGASF
                     NFADYGQDGNPLNPCGDPPGGVGATLTPPTAAGGALRSQDLRTSGDPVTLDGAILRVD
                     PATGAALPNNPLFGHADANAKRIIAYGLRNPFRFTIRPGTSEVWLGDVGWNKWEEIDR
                     IVTPADATVENFGWPCYEGVGRQPGYDGANLNICENLYAAGTAAVNAPYYTYDHAAQV
                     VANETCGTGSSSLAGMAFYEGGSYPDSFDGALFFADYSRKCIWAMEKGTNGLPDPAKR
                     STFVAGAAGPVDLEIGPGGDLFYADFDSGTIRRIQYAAANEPPVADATASPTNGAVPL
                     TVSFDGRGSSDPDGDTLTYAWDLDGDGAYDDSTSSQPVHTYNSAGDYQVGLRVTDGGG
                     AFDTLDQPLTISAGNTPPTATIDSPLSTTTWKVGDNISFSGSATDQQDGALGASKLTW
                     SLIMHHCAPENPSSCHEHVVQDFAGVASGSFVAPDHEYPSYLELRLTATDSGGLTDTK
                     SVRLDPMTVELIFSTEPAGLQLTVGSASATTPFSRTVIVGSKSTMSAPSSQTLAGIPY
                     TFASWSDGGTQSHDIVAPEASATYTATYTATACTMIGTSAADTLTGTSGDDVICGDRG
                     SDIIKGGGGNDILRGEGGADKLYGEDGDDTFDGGLGTDTANFSSSLAAITASLTDNTA
                     TGEDSDTLTDIENLVGSSKDDTLIGSDGDNTINGGGLGDSLTGMGGADTLNGGGGPDS
                     VNSQDGVNGNDSLNGGAGTDTKTTDATEKSIVGFP"
     gene            8268..10883
                     /locus_tag="VHM69_00745"
     CDS             8268..10883
                     /locus_tag="VHM69_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2738943.1"
                     /translation="MRRLALVMISMAVAALLVVVAGNVKRAHALTVVPQGFTQSYVVG
                     PPFSDPRAFDLPHDMVFAPDGRLFVAQQGGIVRILKPDGTLSTFLDISDKVYNSNSFG
                     LLGITLDPQFATNRFVYLFYTAKAEDTNGDGVVDIPLHNVIVRVTANSSGDSAVPGSE
                     QMLLRLDDLEDNGLHNGGSINFGADGKLYASVGENNRKTPAQTLDNFFGKVVRIDKDG
                     TIPTDNPFYDTASGDYRAIWALGLRNPYKMDVQRGTGTIFINDVGGTKWEEINKGASG
                     ANYGWPVREGVANDPFYDDPLFAYPHDETGTLDPNTTGCAIIGSTFYDPLTAQFPSEY
                     AGDYFFSDWCNDWIRRYDPTTDQATQFATEAFNPIDLETGEDGSLYVMQRRGALIKIQ
                     FTGDANRPPSSAIAANPTSGPLPLAVDFDGSGSSDPDAGDTLSYLWDFDNDGTTDETT
                     TTATTSHTYSTKGTYTASLSVRDNHGALSDPDTVRIDAGNEAPNAQIGSPSADLLFKV
                     GKQITLSGSATDPEDGVLPDSAFRWDVLLWHNGSHTHPIFSGTGNNLTFTAPQPEDLF
                     SATGPGNYLEVRFTATDSTGLSKTAIRDVQPNRVNLTFESNPSNPSGLSLEVDHQAFA
                     APKTLVSWEGYKLLVNAPSPQTLSGTPYEFSSWSDAGTQQHEILTGEAPSTYTATYTA
                     TSAACTISGTSGNDTLKGTARNDVICGLGGDDNLKGLGGDDKLLGGDGNDILLGGAGN
                     DAVDGAAGTDRASFAGTTAVSASLATNSATGVGSDTLTAIENLTGSSANDTLIGDSGP
                     NQLSGTGGNDTEHGGGNNDNVVGGGGDDDLYGEDGNDTVNSRDSVAGNDLLDGGTGTD
                     KKVTDATERSVVNIP"
BASE COUNT         2344 a         3514 c         3361 g         2081 t
ORIGIN      
        1 catcgggggc gatttcatcg aggtcttcgt cgacgctcct ctggaggttt gcgccgagag
       61 ggacgtcaag ggtctctaca agaaggcttt ctcgggggag ataccgcagt tcacgggagt
      121 ctcggaccct tacgaggcgc ccgctgctcc cgagcttcac atcaagaccg atgaggagga
      181 tgcccaggcg agcgcgagga aggtcatcgg ctacctcgaa gagctgggct acctcaggcc
      241 cgacagggag ttcagccgcc tgtgaccatt gacgctgctc gcctattggc gcaagctcca
      301 ggtccgaacc cgctcccctg agacggtgag tgtgagagtt ctttcaaaga tccatcgggg
      361 cccgtgggca cataagtcgt ctacaatgtg tcatcatgta catgtgatga cacgcacgaa
      421 taccaactat gctcgaggtt cccgtaagtc tggcgtcgcc ggccgtctga tacgcaccgg
      481 gcgatactta acgggtgtgg tgctagctgg ctacgagctg ttcttaaggt tgccggtagc
      541 gatagtacta gcggtattgt ggttgctggg agcggcgttc ttgggcttgt gtctgttgac
      601 gctctatctg tgcgtaacat tactgatgcg agcgctatga ttagccagag acggcgccaa
      661 gcctcaatcc acaaactgac ttccgtcaca gcctttctct caaggcggca tgaactatta
      721 ccaggtgtca ccaagataat gacagggcag tagccgcggg catcgatctc cccctgctta
      781 ggagtggcac ccctcacctc tggcccgtaa catacataga ggctggcaga acaacccagc
      841 gcggaacggc atggtgcgcg ggtcccagat acttcttgca caaatcgatg aaaggacctc
      901 aacgtgatac ttgtcacggg cggggcgggc tttatcggaa gccacctagt agacaccctc
      961 ttggtcaccg gacagcaact cgtagttttg gacaacctgg ccagcggtta ccgggagaac
     1021 ctgcccaacg ggatcaagtt ggtagagatg gatgttgccg acgccagggt ggtgaagatc
     1081 atagccgagc taaagccgga agtcgtgatc cacgccgctg cgcaggtgag cgtcgcgatc
     1141 tccatgcagg accctcacct cgacctggcc gtcaacgtgc aggggactgc gaacgtgatc
     1201 gagggggcga aggctgccgg tgccaggcgt ttcgtcttcg tctccacggg cgggggggtc
     1261 tacggcgagt ccgatggcgc cgacgaagcc acgcttccgc gccccaagag ctactacagc
     1321 gcgcacaagt acctcgggga gcgctacgtg gagctgagcg ggctctctta tgcgaacgcg
     1381 cggctggcga acgtctacgg caccaggcag cgctctgacc tcgaaggcgg ggtggtagca
     1441 atattcaccg agaggctacg gagtgggcag cccgtcctga tcaacagcac cggcgagcaa
     1501 agtcgggacc tggtctacgt cgcggacgtg gtcgactcgc tcctgaccat ggccaggagc
     1561 gaccgggacg gcacgtggaa cgtcggcacg ggggtctcca ccagcatcct cgagctcctg
     1621 cacgcgctgg aacgggagat cggaccggca acggagatcc gccacggccc cccgcgtccc
     1681 ggggatgtgg gcaactcccg cctggcgatc gaccggatcg agaacgacct ggggtggcgt
     1741 cccaaatatg acctcgccgg tggcatcgcc gacatgatcc ggataacgaa gtaggaccaa
     1801 gatttcgcct ctttcgacag gatggaagaa gggccaaggt ggttagacct cggcccttga
     1861 tcgtcaacaa gacctacaac atcggtgatt tgctctttag ccgaccggcg gggcactggg
     1921 cccgtactcc agcaccgtca cagtatcccc cgttatctga aggtcggccc aggtggagtt
     1981 gttcgtctcg tcactctcca ggaaccggtc ggacccgtct gtcgagtccc cgtcggcctt
     2041 gacgcgtaac ctgtagcgtc cgtcgggcag gcccgttatg tcgaggtact ggccgaccgt
     2101 gccagccccg taggtgtcgc cccaccccct ggagagaccc atcgtcacgt ggagggcacc
     2161 cgggtttccg ccctcgcaac ccaggtatga ctcgggctcg gaagagccat agttagcgtt
     2221 gtcgaagaaa cagaatccta tcttattggc ctcttctacc accgccggct ctacgacgtt
     2281 gccgctatcg tcgagctgga acaggtcata gtcctccagg tccttcacgt gccagtggtc
     2341 gtgcccgtcg ccagagtaga taaaggtggc ggcggtggaa cggtcgcggt agtcgccgac
     2401 ggagtcgaag atgcgttggg tggtggtcat ttctgaggtg ttggtgtcgg ggcgccggcc
     2461 ggtcacctca aatggtccgg ctccgacgtt aacgatcgtc gtggcgaagc gcagttgctt
     2521 ctgagtgccg gtgtcccgga tctgtacgtc ggcgagttgc gccatcccca ggtcgggtaa
     2581 gaggtcttcg ccggtaaggt ttacgttctc gcaggaggca aggacctggt cggtgggatc
     2641 ggcccgaacc gtgtcgttgc ctgtaccgca gttaaacctg tccctctcgg cgatgttacc
     2701 gtctttgtcg tagaggcgat cgtcgtcggc gccgccatta aggatgtcta caccgtctgc
     2761 gcccctcaac tcatcgtcgc cgggtccccc ggccaatgtg tcgttgccgc cttccccatg
     2821 cagcgtgtcg ttgctacctt ggccatacat agtatccgcg ccaaaaccgc cccagagctc
     2881 gtcctggcga cctaagccgt tgatggtatc gttgcccccg aagccgaaga tggtgtccga
     2941 cacgttggtg ccattgagct tgtcgtcgcc agcggtgccg ttgatcacgg cggccagcgc
     3001 cactgagccg acaacgatta gtgcgaatgt tatcgatacg cacagtacaa cgagcttgct
     3061 cactctagcc acctcacttg cgggtcaagg ccttgggatc tttgtctctc tgatcgacga
     3121 ctctcaccgt taccaaccac atcctatacc cgtggtgctt caagcgaatg tgccatcaga
     3181 cctatctttg atcgtctcga tcgagactct agcaacggat acgtgtccca cggcgccagt
     3241 acaggatttc caacgtttta gcccaaagct catccttcgg ttatgagaga tcaacacaat
     3301 agtgcgttcc actacccatc ggcgcggcag ccccacgaac tccttcggtg ggaacgactt
     3361 ctcccaatcg accaccacgc cctcgtgaga ccattgctcg gcccatgggg ctcacctcct
     3421 acctcgaaga gtgctggcga aagggcgaat tgaccatgcc gctcttgtta cacgaggcga
     3481 cgcccgagac gaggtcgcgc tggccgaggg cgttcatcct tcccgtccgc gacgagtcgg
     3541 gaaccttact ggagacgggg ctcgtgctcg aggacttcac cgagggcaag gcggtcgagg
     3601 agcagcccgt ccatcaagcc ttccatgatc ccctgaccgg cctgcccaac cgggcgctct
     3661 tcctggaccg tcggacccac gctctctctc gcgccaagcg ataggccgat cggggcatgc
     3721 ggcgtggcgt tggtgtacat ggacctcgac gacttcaagc gcatcaacga ctccctgggc
     3781 caccaaacgg gggaccgact cctcgtcggg ctggcagaac gcgttggggc ccatttgcgc
     3841 ctcggcgaca cgttcgcgcg ctccgagggg acgagttcgc cttgctgctc gaaaacctag
     3901 aagacgtggg ccaggtcgcc gagaggatcg aacgggccct ggatgttccc ttcggggtgg
     3961 acggccatag ccatgtactg gagcaagagc gagggcaagg cccgccacga ggtcttctcc
     4021 atacagcgca gtacataagt attgatccac ctcgccccac aggcgcagct tggcgaccac
     4081 cgtccggcaa gaccttcctg atgcctgaag cccagcttgg ggaggatcca tccttccgca
     4141 agccgctgta gggatgaacc actcgctgtg gtggacctct gtacgttgtg agcgacccga
     4201 cgtgatgagc gctgtccctc tctgctcagt ggagcggggc aatgcttacg gacgacactg
     4261 acaggtgtgg cccgcaggtt ccctacgcca cagtagtatg tgccacatag cacatataca
     4321 tggtcggttg gcacggctat ccttttcttc ccagttccat atagaaaggt gttggaattg
     4381 atgaagcacg tagcgaacct aggcaccccg cagatgttcc gggtgtggat agtgctagcg
     4441 gctgtaggct tggccggcat tcttgtggcg tcgcttgggc ccctagaggc aaacgctacg
     4501 ggcacgttgc cctccgggtt tcaggaggag gaggttttca gcggcctaac caaccccaca
     4561 aacgtcgagt tctcccaaga cgggcgcgtc ttcgtagccg agaagagcgg cctgataaag
     4621 gtcttcgacg gcctctctga caccacgcca accactttcg cagacctgcg aaccaagatc
     4681 cacaacttct gggatagggg attgctgggg ctcgccctcg cccctaactt ccccagcgac
     4741 ccccacgttt acgtcctcta cacccatgac gccgcgatcg gtggcacggc cccgcgctgg
     4801 ggcacggcga atgtctcttc cgacccctgt cccacccctc cgggcgcaac cgcggacggc
     4861 tgcgtggtga gcgggcgcct ctcgaagctg acggcttcgg gcgacactat gaccggaagc
     4921 gagcaggtct tgatcgagga ctggtgccag cagtatccgt cccattcagt cggagatctg
     4981 gcgttcgggg ctgacggggc gctctacgta agcggcgggg acggggcgag cttcaacttc
     5041 gccgactacg gccaggatgg caaccctctc aacccctgtg gcgacccgcc cggtggggta
     5101 ggagcgactt tgacgccacc aaccgccgca ggaggggcgc tgcgttcgca ggaccttcgc
     5161 acgagcggag accccgtgac gctcgatggt gcaatactgc gcgtggatcc cgctaccggc
     5221 gccgctctgc ccaacaaccc gctcttcggc cacgccgacg ccaacgccaa gcgcatcata
     5281 gcctatgggc tccgcaaccc cttccgcttc accatacggc ctggcacgag cgaggtttgg
     5341 cttggcgacg tcggctggaa caagtgggag gagatcgatc gcatagtcac gcccgccgac
     5401 gccaccgtcg agaacttcgg ctggccttgc tacgaggggg tcggacgcca accgggctac
     5461 gacggtgcca atctcaacat ctgcgagaac ctctatgccg cggggacggc ggccgtcaat
     5521 gctccatact acacctacga tcatgccgct caagtggttg cgaatgagac gtgcggtacg
     5581 ggcagttctt cgttggccgg gatggctttc tacgaaggag gctcctaccc ggacagcttc
     5641 gacggcgccc tgttctttgc cgactactcc cgcaaatgca tctgggcgat ggagaaaggc
     5701 acaaacgggc tgccggaccc agccaaacgc agcaccttcg tagcgggagc ggcgggtccg
     5761 gtcgatctgg agataggacc ggggggagat ctgttctacg ccgacttcga cagcgggacg
     5821 attcgccgca tacagtacgc ggccgccaac gagccccccg tggcggacgc caccgcgagc
     5881 ccgaccaatg gcgcggtacc cttgacggtg agcttcgatg gcagaggctc gagcgacccc
     5941 gacggcgaca cgctcaccta cgcttgggat ctcgacggcg acggagccta cgacgactcg
     6001 acctcctcgc agcccgtcca cacctacaac tccgcgggag actaccaggt ggggctcagg
     6061 gtcaccgacg gaggcggggc attcgacacc ctcgatcagc cgctcacgat aagcgccggc
     6121 aacacaccgc caacggcgac tatagactct cccctctcaa ccaccacctg gaaggttggg
     6181 gacaatatct ccttctccgg ctcagccacc gaccagcagg atggagcgct gggggcttcg
     6241 aagctgacct ggtcgttgat catgcaccat tgcgcacccg agaacccttc ttcttgccac
     6301 gagcacgtgg tgcaggactt cgcgggcgtg gcgagcggct cgttcgtcgc tccagaccac
     6361 gagtacccct cttacctgga gctccggctc accgctacgg actccggcgg tttgactgac
     6421 accaagagcg tgcggcttga ccccatgacc gtggagctga tctttagcac cgagccggca
     6481 gggctacaac tgaccgtcgg cagcgctagc gcaacgacgc ccttctcacg cacggtgatc
     6541 gtcggctcga agagtacaat gagtgccccc tcgtcccaga cgctggcggg gatcccctac
     6601 actttcgcct cctggtccga tggtgggaca caaagccacg acatcgtagc acccgaagcc
     6661 tcagccacct acacggccac ttacacggcc acggcctgca ccatgatcgg cacctcggct
     6721 gcagacaccc tcaccggcac ctcgggagac gacgtaatct gcggagatag aggcagcgac
     6781 atcataaagg gcgggggcgg caacgacatc ctcagaggcg aggggggagc agacaagctc
     6841 tacggcgaag atggcgacga taccttcgac ggcggcctgg gtaccgacac cgctaacttt
     6901 tcaagctcct tggcggccat aaccgcctcg cttaccgaca acacggcaac cggagaggac
     6961 tccgataccc tgacggatat agagaacctg gtgggttcct ccaaagacga caccctcatc
     7021 ggctcggatg gcgacaacac cataaacggc ggagggttgg gggacagcct caccggcatg
     7081 ggtggggcgg acacgctcaa cggcggaggc ggtcccgact cggtcaactc ccaggatggc
     7141 gtaaatggca atgactccct caacggcggg gctggcaccg atacaaagac gacggacgcc
     7201 accgagaaat cgatcgtagg gttcccatag gcttgggact ggatttctag agcgcctgtc
     7261 atagcagttg atccatcgga cgatagccgc agtgtccgaa gaacgctcga gcaactcttg
     7321 ccgtaaccgc agcgtccagc ccatcgcctc gatcagcgcc tctcgggtgc gcgcctcaac
     7381 tatgaccttc gagagcgtcg ctccaagtca agaggcgctc acccggacac tttcgcccta
     7441 ctgggaagat cccgcgctcc tcaagcgtct caccggatac caatggtggg tagaggcggt
     7501 ggagtcgtta tggcatcagt agaccgggac ggtattagac cggggcaatc tacatgttgt
     7561 ggtgctgagt agatatgcag gatcttggtt catgtaagaa gacctccatg attttcagtg
     7621 ttattggact aacaccgagc gtagggaggt cttttctatt gctcaagtct tcacgagaaa
     7681 cgtgcacacg gggttaattc agaagttagc tacaagcttg caacccccaa gcaactgcaa
     7741 gaatacgcca tctggcagat gtgccgagtc tcacacgcac ccattatgcc tacctaagaa
     7801 aacttggcca cccattgcgg gtagaggcta agaacctcct ccctcaagta tgggcactct
     7861 agtagtgacg agccactggt gcaaccggct acctcacaca tcaagaggtt gtccggtctt
     7921 gttttggaga gcggcggaaa ggggcgaacc gagtaagaaa ccatcgaaag aagtgggtca
     7981 aaggaagaca aggagagtag gatcaataga tatgcacttg caccgcaagt tgctggtagt
     8041 ggatgtggcg ctttcgctgg ccgtgacgtt tgtgttaccc aaggtaagta cggtcgaagc
     8101 gcccgaccca gtcgtcgatg gattttgggc aggggctgcg atcgtaatcg tcaccgggcg
     8161 aaacccttga tacgaacagc cttccaaaaa tgacctctat tgcgaatata cacactcctg
     8221 aatgatggta ctgctgcgac acagatgttc accgaggaga tgaggaaatg agaaggttgg
     8281 cgttggtgat gataagcatg gccgtggccg cgctgctagt agtcgtggcg gggaacgtga
     8341 agcgcgcgca cgctttaact gtggtcccgc agggcttcac gcagtcgtac gtggttggcc
     8401 cgcctttcag tgatcctaga gcatttgacc tccctcacga tatggttttc gcgcccgacg
     8461 gacgactgtt cgtggcccaa cagggcggca tcgtgcgtat attgaaaccg gacgggacgc
     8521 tatctacctt ccttgacatc tcggacaagg tctacaacag taattccttt ggtctccttg
     8581 ggatcaccct cgatccccag ttcgccacca accgcttcgt ttacctcttc tacacggcca
     8641 aggcggagga cactaatggc gatggagtag tagacatccc gcttcacaac gtcatagtcc
     8701 gcgtcacggc caactccagc ggagacagcg ccgtacccgg cagtgagcaa atgctcctcc
     8761 ggctggatga cctcgaggac aatggcttgc acaacggtgg ctcgatcaat ttcggggccg
     8821 acggcaagct ctacgcctcc gttggcgaga acaatcggaa gactcccgcg cagactctgg
     8881 acaacttttt cggcaaggta gtaaggatag acaaggacgg cacgatcccc acggacaacc
     8941 ccttctacga cacggcttcg ggcgactacc gggctatctg ggccctgggc ttgcgcaacc
     9001 cctacaagat ggacgtccaa cgcggcaccg gcaccatctt catcaacgac gtgggtggca
     9061 ccaagtggga agagatcaac aagggcgcgt ccggcgctaa ctacggctgg cccgtacgcg
     9121 agggcgttgc gaacgatccc ttctacgacg acccgctctt cgcctacccg cacgatgaga
     9181 cgggtaccct tgaccccaac accacgggtt gcgcgatcat cggcagcacg ttctacgacc
     9241 ctctaacggc acagttcccg tcggagtacg cgggcgacta cttcttcagc gattggtgca
     9301 acgactggat acgtaggtac gatcccacca cggaccaggc gacccagttc gccaccgaag
     9361 cgttcaaccc catcgatctc gagacgggtg aggacggcag cttgtacgtg atgcaaaggc
     9421 gcggagccct gatcaagatc cagtttaccg gcgacgccaa ccgccccccg agctcagcca
     9481 ttgcggcgaa cccgacctcc gggccgctgc ccttagcggt cgacttcgac ggctccggga
     9541 gcagcgatcc cgacgctggc gatacgctga gctacctctg ggacttcgac aacgacggga
     9601 cgacggacga gacgactacc actgcgacga cgagtcacac ctactcgacg aagggaacct
     9661 acacggcctc gctgagcgta agggacaacc acggcgcact atcagacccg gacacggtcc
     9721 gcatcgacgc gggcaacgag gcgcctaatg cccagatagg ttcgccctcc gctgacctgc
     9781 tcttcaaggt cggcaagcag atcacgctga gcggcagcgc gaccgacccc gaggatggcg
     9841 ttctgccgga cagcgccttt aggtgggacg tactcttatg gcacaatggc agccacacgc
     9901 accccatatt ctcggggacc ggcaacaacc tgacgtttac cgccccgcaa ccggaagatc
     9961 tcttctccgc aaccggccct ggcaactacc tggaggtaag atttacggca accgactcca
    10021 cgggcctctc caagacggcc atccgagatg tgcagcccaa ccgggtgaac ctcacgttcg
    10081 agagcaaccc gagcaacccg agcggtctgt cgctggaagt cgaccaccag gcattcgccg
    10141 ctccgaagac gctagtatcg tgggaaggct acaagctgct cgtaaacgct ccctcaccac
    10201 agacgctctc aggcacacct tacgagttct cttcctggtc cgacgccgga acgcagcaac
    10261 acgagatcct taccggagaa gcaccgagca cctacacggc cacctacacg gccacctcgg
    10321 cggcctgcac gatcagcggc acctcgggga acgacaccct caagggtact gccagaaacg
    10381 acgtcatctg cggcctgggc ggcgacgaca acctcaaagg tttgggcgga gacgataagc
    10441 tcctgggcgg agacggcaac gatatcctcc tcggcggagc cggcaacgat gccgtcgatg
    10501 gcgcggccgg cacggacagg gcttcgttcg cgggaaccac ggctgtcagc gcttcgttgg
    10561 ccacgaacag tgccaccggt gtgggctccg acacgctcac ggcgatagag aaccttaccg
    10621 gctcgtcggc caacgacacc ctcattggtg atagcggtcc taatcagctc agcgggaccg
    10681 gcggcaatga caccgagcac ggaggcggga acaatgacaa cgtcgtaggc ggcggcgggg
    10741 acgacgacct ctacggggag gatggcaacg acacggtcaa ctccagggat agcgtggcgg
    10801 gcaacgatct cctcgacggg gggacaggca cggataagaa ggtaacggat gccaccgaga
    10861 ggtcggtcgt gaatatcccg taagcgccag gaacaccacc gaggtagtca ttgctggggc
    10921 cggggctatt ggtagtagcc ccggtccttc atatctccct tattcgccaa acctcataga
    10981 aggatctgca caatgcggat gcacgatctt ggatagaaca cccctgagag gcgcgaggat
    11041 ggctcctgag gccactcgtg cagcccctcc taagtttagg ttgtgcataa ggatccgagc
    11101 gtgtgaacag cgctaggact tattgggcat ccggatttct tggccctcgc cctgtcgtaa
    11161 accttgggtg accagcttcc tcaccttggc ctgatgccag atccacacgc cggtgatgat
    11221 gatcacggga accaagatga tccacactat ggcagttctc gaaaaggatg agctactcct
    11281 tcggagaagg ctacaggcta
//
LOCUS       DATAIR010000027         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416730, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000027 DATAIR010000000
VERSION     DATAIR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416730"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHM69_00750"
     CDS             <1..299
                     /locus_tag="VHM69_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX2738944.1"
                     /translation="SLGLQMSGLLEGEYDAQGRPEEDDDFLLLFNAGDEEVDFQIPPF
                     PEEARWEVFMDTAYSAGLKPDGYFKPGGVYTLKSRSMVVLINARPRETVPEDAD"
     gene            296..>1577
                     /gene="treZ"
                     /locus_tag="VHM69_00755"
     CDS             296..>1577
                     /gene="treZ"
                     /locus_tag="VHM69_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX2738945.1"
                     /translation="MRRLHRMPFGAEVLENGETRFTLLAPAAETVDLILEDDLLSMHR
                     EEDGTFVLTTGAGAGARYLYRIDGTQEVPDPASRYQPDDVHGASQVVNPAAFVWEDEH
                     WKGRPWEETALYELHVGSFSPEGTFAGVKEKLDHLVDLGVTAIELMPLSEFPGRRNWG
                     YDGVLPFAPESAYGTPEDLKDLVQTAHSKGIMVFLDVVYNHFGPEGNYLPLYAPQFFT
                     DRYDTPWGAAINFDGEGSEQVREFFIHNALYWLEEYNLDGLRLDAVHAINDASEKHFL
                     DELAERVRQGPGRERHVHLVLENETKDVHYLRGPYDAQWNDDFHHALHVTLTGEDASY
                     YADYADKPLRHLGRCLAEGFALQNEPSGGLPPGAFVSFIQNHDQIGNRAFGDRISHLA
                     PLEAVRTAAEIYLLAPQIPMLFMGEEWAARTPFTF"
BASE COUNT          304 a          519 c          478 g          276 t
ORIGIN      
        1 ggtccctggg cctgcagatg tcgggtcttc tcgaaggcga atacgacgcc cagggccgtc
       61 ccgaggagga cgacgacttc ctcctcctct tcaacgccgg ggatgaggaa gtcgacttcc
      121 agatcccacc cttccccgaa gaggcccgct gggaggtctt catggacaca gcctactccg
      181 ctggactgaa gcccgacggc tacttcaaac ccggcggcgt gtataccttg aagtcccgct
      241 cgatggtcgt cctgattaac gcccgcccgc gtgagaccgt ccctgaggac gcggattgag
      301 gcgcctccac cggatgccct tcggcgctga ggtactggag aacggagaga cccgcttcac
      361 cctcttggca ccggccgcgg aaaccgtaga tctcatcctc gaagatgacc ttctctctat
      421 gcaccgcgag gaggacggaa cttttgtcct cacaaccgga gctggtgccg gtgcgcgcta
      481 cctctaccgt atagacggaa ctcaggaggt gcccgaccct gcctcccgtt accagcccga
      541 cgacgtgcac ggcgctagcc aggtcgtcaa cccggcggcc ttcgtctggg aggacgagca
      601 ctggaaggga cgtccgtggg aggagactgc tctctacgag ctgcacgtcg gctccttcag
      661 ccccgaaggg acgttcgcag gggtcaaaga gaagctcgac cacctggtag acctcggcgt
      721 gacggcgatc gagttgatgc cgttatccga gttccctggg cggcgcaact ggggctacga
      781 cggcgtattg cctttcgccc ccgagagcgc ctacgggacg ccggaggatc tgaaggatct
      841 cgtacagacc gcgcactcga agggcatcat ggtcttcctc gacgtcgtct acaaccactt
      901 cggcccagag gggaactacc tcccccttta tgccccgcag ttcttcacgg accgctacga
      961 cacaccgtgg ggggcggcga tcaacttcga cggcgagggc agcgaacagg tgcgcgagtt
     1021 ctttatccat aacgcgctgt actggctgga ggagtacaac ctcgacggtc tcaggctcga
     1081 cgcggtgcac gccataaacg acgcgtccga gaaacacttc ctcgacgagc tggccgagcg
     1141 cgtgcgccag gggccaggac gcgagaggca cgtgcacctc gtacttgaga acgagacgaa
     1201 agacgttcac tacctgcgcg gtccgtacga cgcccagtgg aacgacgact tccaccacgc
     1261 gctgcacgtc accctgaccg gcgaggatgc cagctactac gcggattacg ccgacaagcc
     1321 cttgcggcat ctgggcaggt gccttgccga agggttcgcg ctccagaacg agccgagcgg
     1381 gggactgccg cccggtgcct tcgtttcgtt catccagaac cacgaccaga tcggcaaccg
     1441 ggcatttggt gacaggatct cacacctcgc accgctcgaa gcggtgcgta cagcggcgga
     1501 gatctacctt ctcgcgcccc agattccgat gctatttatg ggtgaagagt gggccgccag
     1561 gacccccttc acgttct
//
LOCUS       DATAIR010000028         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_416797, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000028 DATAIR010000000
VERSION     DATAIR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416797"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHM69_00760"
     CDS             complement(<1..227)
                     /locus_tag="VHM69_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEX2738946.1"
                     /translation="MILDFERPIKDFEERIAELRRLAGPSTELRGEISRLEEALDAAR
                     RRIYSNLSPYQRVQVARHPERPNFRAYRDALA"
     gene            complement(224..994)
                     /gene="accD"
                     /locus_tag="VHM69_00765"
     CDS             complement(224..994)
                     /gene="accD"
                     /locus_tag="VHM69_00765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX2738947.1"
                     /translation="MDSVFTKCEKCGQPIYEKDLRARFNVCPNCEFHYPLSAPERIRL
                     LTDVGSFEERDGGIVADDPLGFEDYRQRLEKARSKTGLDDAILNGTGAIGGRRVALAV
                     LDFRFIGGSMGSVVGERVARTIELAGSEELPLVIVSASGGARMFEGVYSLMQMAKTSV
                     ALSRYVDGSSPYVSILTDPTFGGVTASFATAADVVIAEPGARIGFTGARVIEQTTRER
                     LPDGFQTAEFQREHGMVDRIVHRLTLKGDLERLLGFLP"
     gene            complement(1068..1853)
                     /gene="trpA"
                     /locus_tag="VHM69_00770"
     CDS             complement(1068..1853)
                     /gene="trpA"
                     /locus_tag="VHM69_00770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX2738948.1"
                     /translation="MNGGGRLRQAFREGRTALVPYLTGGYPTLEGAQEVGEAYLEAGA
                     DVIEIGVPFSDPLADGPTIQDTTTRALRNGADLEYCLGLASEFSGRVPVVFLLYYNVI
                     FARGVERFLREAAEAGVSGLVIPDLPVDEAGRFAELAADAGVAFCPLVAPTSTDERVS
                     RVGEMATGFVYCVSVAGVTGVRDTLPPGAVELLRRVRSRTDAPVALGFGIGSSRAAVE
                     AAGEADGIIIGSKLMSLVEEGGAQRAGEWLREVRDALSMKAVG"
     gene            complement(1850..>2628)
                     /locus_tag="VHM69_00775"
     CDS             complement(1850..>2628)
                     /locus_tag="VHM69_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2738949.1"
                     /translation="NVVRMKLLGAEVVPVLSGSRTLKDAVNEAIRDWVANVEDTHYII
                     GSVVGPAPYPRIVRDFQAVIGTEMEAQCRERFGGDPDAIVACVGAGSNAIGAFHPFVG
                     KGNVRLVGVEAAGRGLATGEHGASLAEGKLGVLHGAMSYVLQTEGGQIREAHSISAGL
                     DYPATGPEHAYLKDEGLAEYTSVTDDEALEALQTCSRLEGIIPALETAHAIFYAEKVA
                     RELGPGKDMVVNISGRGDKDVEVAAEALGIGSEEADEVSS"
BASE COUNT          436 a          946 c          795 g          451 t
ORIGIN      
        1 gcgagggcgt cgcggtacgc gcggaaattc ggacgctcgg ggtggcgggc cacctggacg
       61 cgctggtacg gagagaggtt cgagtatatg cggcgcctgg ccgcgtcgag ggcctcttcg
      121 aggcgtgaga tctccccgcg cagctcggtg ctcgggccgg cgagccgcct gagctcggcg
      181 atacgctcct cgaagtcctt tatcgggcgc tcgaaatcga ggatcacggc aagaacccca
      241 gtagccgttc gaggtcccct tttagggtca gacgatgcac gattcggtcg accatcccgt
      301 gctccctctg gaactcggcc gtctggaaac cgtccggcaa cctctcacgg gtcgtttgct
      361 cgatgacccg agcacccgta aagccgatcc tggcccctgg ctcggcgatc accacgtcag
      421 ccgccgtggc gaacgatgcc gtcaccccgc cgaaggtggg atccgtgagg atggagacgt
      481 atggcgacga cccgtctacg tagcgcgaga gcgcgacgct cgtcttggcc atctgcatga
      541 gcgagtagac gccctcgaac atccgcgccc cgcccgaggc ggagacgatg acgaggggca
      601 gctcctcaga accggccagt tcgatggtcc tcgcgaccct ctccccgacc acgctcccca
      661 tcgacccccc gataaaccgg aagtcaagga cggcgagcgc caccctgcgc cctcctatgg
      721 cgcctgtccc gttaaggatc gcgtcgtcga gccccgtctt actgcgcgcc ttctccagcc
      781 gctggcggta atcctcgaag ccgagagggt cgtctgcaac gatgcccccg tcccgttcct
      841 cgaagctgcc cacgtccgtg aggagcctta tcctctcggg cgccgagagc gggtagtgga
      901 actcgcagtt gggacagacg ttgaagcgag ccctgaggtc cttctcgtag atcggctgcc
      961 cgcatttctc acatttggtg aagacggagt cgatcgggcc aacgccgctc tcggtctcag
     1021 gcgcgctcct cgaatactcc ctgcgtttgc tgagccagtg cctgatacta tcccaccgcc
     1081 ttcatggaga gcgcatcgcg aacctcgcgc agccactccc ccgccctctg cgccccgcct
     1141 tcctcgacca gactcatcag cttgctcccg atgatgatcc catccgcctc ccccgctgcc
     1201 tcgaccgcgg cccggctcga cccgatcccg aatcctaggg caacaggcgc atcggttctc
     1261 gaacgaacgc gtctcagtag ctcaacggca ccgggaggca gtgtgtcgcg caccccggtc
     1321 acccccgcta cggaaacgca gtacacgaac cccgtcgcca tctccccgac gcgggagacg
     1381 cgttcgtccg tcgacgtggg cgccacgagc gggcagaagg cgacgcctgc gtcggccgca
     1441 agctcggcga accggcccgc ctcgtccacc gggaggtcgg ggatcacgag tccggagacg
     1501 cccgcctcgg cggcctccct caggaaacgc tcgacccccc gcgcgaagat gacgttgtag
     1561 tagagcagaa ataccacggg cacacgcccc gagaactcag aggcgagacc gagacaatac
     1621 tctaaatcag cgccgttccg aagggcgcgc gtggtggtgt cctggatcgt cggcccgtcg
     1681 gcgagcgggt cagagaaggg gaccccgatc tcgatcacgt cggcaccagc ctccaggtag
     1741 gcctccccca cctcctgcgc cccttcgagc gtcggataac cgcccgtcag gtaaggcacg
     1801 agcgcggtcc gtccttcccg aaaagcctgc ctcagacgcc cgccaccgtt cacgaggaga
     1861 cttcgtccgc ttcttcggag ccgatcccca gggcctccgc cgcgacttcg acgtccttgt
     1921 cgccgcgccc ggagatattg acgaccatgt ccttgccggg gccgagctct ctggccacct
     1981 tctcggcata gaagatggcg tgcgccgtct ccagggcggg gattatgcct tcgaggcggg
     2041 agcacgtctg gagcgcttcg agggcctcat cgtcggtcac gctggtgtac tcggcgagac
     2101 cctcgtcctt gaggtaggcg tgctcaggtc ccgttgcggg gtaatcgagg cccgctgaga
     2161 tcgagtgtgc ctcccggatc tggcctccct ccgtctgcaa cacgtagctc atcgccccgt
     2221 gcaatacgcc gagcttcccc tcggccagtg aagccccgtg ctcccccgtc gcgagcccac
     2281 gccctgcggc ctctactccg acgagcctga cgttcccctt cccgacgaac gggtgaaatg
     2341 ctcctattgc gttcgagccc gcgccgacgc aggcgacgat agcgtcgggg tcgccgccga
     2401 agcgctcgcg gcactgggcc tccatctccg tccctataac ggcctggaag tcgcgcacta
     2461 tccttggata aggcgctggt cctacgacgc tcccgataat gtagtgcgtg tcctcgacgt
     2521 ttgcgaccca gtcccttatc gcctcgttca cagcgtcttt gagggtgcgc gagccgctga
     2581 gcacgggcac gacctcggca ccgaggagct tcatccgcac gacgttca
//
LOCUS       DATAIR010000029         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_417143, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000029 DATAIR010000000
VERSION     DATAIR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417143"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VHM69_00780"
     CDS             <1..1614
                     /locus_tag="VHM69_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2738950.1"
                     /translation="AKARRNIVLGRMLEYGYISKQEHDKAVASDVVLSRGRVQYEYDN
                     EYFLNAVRKEIAEEYGDDMVYEGGLKIYTTLDPDLQAMANTAVDSIVNPEAGDPSAAL
                     VSIDPATGAVRAMVGGSDFDQVKFNLATQGHRQAGSSFKPFVYAEAVHEGISPETMYI
                     SKHLYVDMGKNAEPYEVDNYDFIHRGAITLEKAMADSDNTVFVQLALDIGLQKVVEMA
                     NDLGVDSEVDAYPSTAIGGLRVGVTPLEMASAYSTFANSGRHMEPYLVQKVTKEENGK
                     EVLLEEHRLVGEEVLSRDEAAVVTEALRAVVERGTASYYHDLDAEIGRPAAGKTGTSN
                     EFIDAWFIGFIPQLSTSVWVGYPNERRSMVNINGLEQINGENYPLDIWSLYMQSAVQK
                     YPVQEFDAPSPDMDLEVKTDGRAYVKPPPPPPEENEEDEKADEEKTKEEKTKEEESNN
                     EEDTEKPDVQPEETGPDQEPPPQQAPASASPSAASPAPSSAAPARAAPAPAAPARAPR
                     AQNGGRKFGPNARPNVGPRFGRVGPVRRR"
     gene            1634..2806
                     /locus_tag="VHM69_00785"
     CDS             1634..2806
                     /locus_tag="VHM69_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2738951.1"
                     /translation="MNHAHSQARGGFARDPATWMSYGLVGYFAFMETVLGPIMPFLRK
                     EMDLGYTTASLHFSAFALGAVMLGFFGDRVVGSWGRRAALWGGAAGMAAGALLLVSSP
                     SVFGTVPAAFAMGICGAQVLVTTQALLSDTHGEWSAVALTESNVTASACAIAAPLLVG
                     ASAALGLGWRAALVLPAVALALLAAGFFSRPLDPPRNATDGAPAGGRTLPPRYWAFWT
                     LVALGVASEWCVAYWGADFLADGTGLSRPAAATSLTAFFVAMLTGRIVSSRLTRTLPP
                     TVLLIATLCAALVGFPLFWLSPGITLTLVGLFITGLGIGSVYPLGVSAAIATAPDNAD
                     AAAARLAVGGGGAILVAPFVLGALADRIGIAGAFGIVVPMLLAAVSLALFAGRKSR"
BASE COUNT          536 a          996 c          956 g          479 t
ORIGIN      
        1 gcgaaggcga gacgcaacat cgtactcggc aggatgctcg agtacggata catctccaaa
       61 caagagcacg acaaggccgt ggcctcggac gtcgtcctga gccgtggcag ggtccagtac
      121 gagtacgaca acgagtactt cctcaacgcc gttagaaagg agatcgccga ggagtacggc
      181 gacgacatgg tctacgaggg cgggctcaag atctacacca cactcgaccc cgacctgcag
      241 gcgatggcga acaccgcggt cgactccatc gttaaccctg aggccggcga cccttccgcg
      301 gccctcgtct cgatcgaccc ggcgacgggc gcggtcaggg ccatggtcgg cggttcggac
      361 ttcgatcagg tgaagttcaa cctcgcgaca caggggcatc ggcaggcagg cagctcgttc
      421 aaaccattcg tctacgccga ggccgtacac gaggggatct cacctgagac catgtacatc
      481 tccaagcacc tgtacgtaga catgggtaag aacgccgaac cctacgaagt ggacaactac
      541 gacttcatcc accgcggcgc catcaccctg gagaaggcga tggccgactc tgataacacg
      601 gtcttcgtcc agctcgccct cgacatcggg ctccagaagg tggtcgagat ggcgaacgat
      661 ctcggcgtgg atagcgaggt cgacgcctac ccgtcgaccg cgatcggggg cctgcgtgtc
      721 ggggtgacgc cgctggagat ggcctcggcc tactcgactt tcgcgaactc gggcaggcac
      781 atggagccgt acctggtcca gaaggtgacg aaggaggaga acgggaagga agtgctcctg
      841 gaggagcacc gcctggtcgg cgaggaagtc ctgagccgcg acgaagccgc agtcgtcacc
      901 gaggccctcc gcgccgtcgt cgaacgcgga acagcaagct actatcacga cctcgacgcc
      961 gaaataggcc gccccgccgc aggcaagacg ggtacctcca acgagttcat agacgcctgg
     1021 tttatagggt tcattccgca gctatccacg agcgtctggg tcggttaccc caacgagcgc
     1081 cgctccatgg tgaacattaa cggcctggag cagatcaacg gcgagaacta cccgctggac
     1141 atctggtccc tctacatgca gagcgccgtc cagaagtacc ccgtgcaaga gttcgacgcc
     1201 ccgagccccg acatggacct cgaggtgaag accgacggac gcgcctacgt gaagccccca
     1261 ccgcctccac cggaggagaa cgaggaagac gagaaagccg acgaagagaa gaccaaagaa
     1321 gagaagacca aagaagagga gtccaacaac gaggaagata ccgagaaacc ggatgtccag
     1381 ccagaagaaa caggcccgga tcaggaacca cccccgcaac aggctccggc gagcgcttct
     1441 ccatcagcag catctcccgc gccctcgtcg gccgcgcccg cccgagccgc gcccgcacca
     1501 gccgcgcccg cgcgggctcc gcgggcgcag aacggaggca ggaagttcgg gccgaacgca
     1561 aggccgaacg tcggcccgcg attcggacgg gtaggtccgg tgcgtcgaag gtgagtcttt
     1621 ggggtggagc gacgtgaatc atgcacacag ccaggcccgg ggcggcttcg ccagggaccc
     1681 tgccacctgg atgtcctacg gcctggtcgg ctacttcgca tttatggaga cggtactcgg
     1741 tccgatcatg cctttcctca ggaaggagat ggaccttggc tacaccacgg caagcctcca
     1801 cttcagcgct ttcgcccttg gcgccgtcat gcttggcttc ttcggagaca gggtcgttgg
     1861 gagctggggg agacgggcag ccctctgggg cggcgcggcg ggcatggccg caggcgccct
     1921 gctcctggtc tccagcccga gcgtcttcgg caccgtaccc gcggccttcg ccatgggcat
     1981 ctgcggtgcc caggtcctcg tcaccaccca ggcccttctc tccgacacgc acggagagtg
     2041 gagcgcggtc gccctaaccg agtcgaacgt aacggcgagc gcgtgcgcga tcgccgctcc
     2101 gctgctcgtc ggcgcgtccg cggcgttggg cctcggctgg cgtgcggcgc tggtccttcc
     2161 cgcggtggcc ctcgctctgc tcgccgcggg tttcttctcc cggcccctgg atcctcctcg
     2221 gaacgcgacg gacggcgcgc cggccggtgg acggaccctg cccccgcgct actgggcgtt
     2281 ctggacactg gtggcgctgg gtgtcgcctc ggagtggtgc gtcgcgtact ggggggctga
     2341 ctttctcgcc gacggaaccg gcctctcccg cccggctgcg gccacctccc tgaccgcctt
     2401 cttcgtcgca atgctcactg gccgcatcgt gagcagcagg ctgacccgca ctctgccacc
     2461 gacggttctc ctcatcgcga ccctctgcgc ggcgctggta gggtttccgc tgttctggct
     2521 ctcgccgggg atcaccttaa ccctggtagg actcttcata accggacttg gcatcggaag
     2581 cgtctacccg cttggcgtct ccgcggcgat cgctactgcg cctgacaatg ctgacgcggc
     2641 agcggcgagg ctcgccgtcg gagggggcgg cgctattctc gtggccccct tcgtactcgg
     2701 agccctggcc gaccgtatcg gcatagccgg cgccttcggc atcgtagtcc cgatgctcct
     2761 cgcagccgtc tcgctggccc tcttcgccgg acgaaagagt cgctgagagc gcggtcaggc
     2821 gctgtgcagc gtctccagga cttcctggtg caggttggcg ttggtggcga ccagtccgtc
     2881 cgaggagagg ttccacggcc cgcccgagag gtctgtggct cggcccccgg cctcggagac
     2941 taggatgacc gtcggtgcgt agtccca
//
LOCUS       DATAIR010000030         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_139281, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000030 DATAIR010000000
VERSION     DATAIR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139281"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM69_00790"
     CDS             <1..318
                     /locus_tag="VHM69_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEX2738952.1"
                     /translation="LEDLGINAPELRLAGGGTGGSSGGPWRQLLADVLGRPLRLLPDE
                     VSAVASARGAAFLAGIASGVYQAAGDTLALTPEPEHSILPGEADYEAAYVRYTELYPR
                     LYR"
     gene            423..1844
                     /locus_tag="VHM69_00795"
     CDS             423..1844
                     /locus_tag="VHM69_00795"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX2738953.1"
                     /translation="MLSREYAGSRGETGAPEGARWLLADRVSRVLSGANGVTNPTGRV
                     VSLAVPVGRIDAFRWLREQRTFPKTYWSGREDEAGVAAVGAADLRESGVSEGPGSLSR
                     LLAALPDAGVSEARYYGGMRFDPLRKPDEEWRAFGAYRFVLPRFELHAGEGETALVCN
                     LLLPRDADNAPEILREIEDLALPPGTSDPSLPIPVSRQDSPGLRGWRENVEQAISAFA
                     EGRLGKVVLARRAEFGFDGELDPIFLLESLKAATPGCFHFYAEPKDGAVFLGASPERL
                     YRRDGRSVVSEAVAGTRPRGASSADDDGLRDDLLHSAKDLSEHSYVRIGIQEALAPHC
                     KELEMDQDISEMKLARGRHLRSKVRGTLREGVTDAGLLDALHPTPAVGGHPRGEAMEE
                     INALEPFDRGWYAGPVGWIGAEASEFAVGLRSGLVRGRTLALFSGAGIVAGSAPDEEW
                     AEIEQKIGDFTGMFGLEQGHAAS"
     gene            1819..3570
                     /gene="menD"
                     /locus_tag="VHM69_00800"
     CDS             1819..3570
                     /gene="menD"
                     /locus_tag="VHM69_00800"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX2738954.1"
                     /translation="MNRDTPRANRLWAYLIVEELLRCGVEFFCVAPGSRSTPLVAALA
                     ANEKARSLIHFDERGTAFAALGYARATRRPAAWITTSGTAVANGLPAVVEAATDGVPM
                     ILLTADRPPELRQTGANQTIDQPDIFGDYVRWRFDLPAPDPVIDPAMVLTTVDQAVYR
                     AMRTPQGPVHLNLMFREPFLPEDEDSPFAPSSWTKSGEPYTRYAATKPTVDQTELDLL
                     WEAIRLTKRGLVVAGRLASRKQGEAVMRLAETLGWPLLPDIGSQIRLGADSEGFVAHY
                     DALLSSDSFAGAHAPEAVVHVGGRALSKRLEQFLARSRPDPYVVVRENPFRLDPSHRV
                     THSVEADVVDFCAALARSATRDPAAVDATWAAGWQEASEETGHHLDEIFSGTQEPDEP
                     QVARAVSRNIPLDHGLIVASSMPVRDLDTYAATDGAPVPVAANRGASGIDGTVATAAG
                     FAHGSGRPVTLLIGDLALLHDMNSLAMLSDLHVVVVVLNNDGGGIFSFLPVASQKEFF
                     EPYFGTPQGVGFEHAAAMFGLGYERPGTMDEFVESYRVACSRGYSTLIEVHTDREENV
                     ALHRRILEEIVARTEKG"
     gene            3697..4494
                     /gene="menH"
                     /locus_tag="VHM69_00805"
     CDS             3697..4494
                     /gene="menH"
                     /locus_tag="VHM69_00805"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEX2738955.1"
                     /translation="MRRQNRLNFEVTGDSGSPAVLFLHGFMGSSADWREITTALEVRS
                     LCIAVDLPGHGASLGMGPEAYTIDGAARAVICTLDELGIERPVIAGYSMGGRLALYLA
                     LRYPERCSGLFLESTSPGLERAEERTARREADEEKAKRLESRDFEEFLEDWYRQPLFA
                     SLASDEKLLRRTIEARLRNDPAELARSLRRMGTGSQPSLWGELERLAIPALSVAGVLD
                     EKYAGISSRMAGLSPWMESVVIPETGHTVHAEAPAVYVSLLLRFMED"
     gene            complement(4491..4907)
                     /locus_tag="VHM69_00810"
     CDS             complement(4491..4907)
                     /locus_tag="VHM69_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2738956.1"
                     /translation="MIQRQSHSTVYVIDQDEALKFYRDILGFEVNTDMTMDDSSFRWL
                     TLNPKGQPDLEVILAKVSSSPMFDADTAEEMKALVARGAFGIGVFETDDIHGDYERLS
                     KQGVEFVSPPKEQFYGTEAIVKDNSGNWFSLTQPKS"
     gene            complement(4911..5351)
                     /locus_tag="VHM69_00815"
     CDS             complement(4911..5351)
                     /locus_tag="VHM69_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2738957.1"
                     /translation="MRYAFRRPVSLPDVAAQANLSPYHFLRVYKGVYGETPHEFLTRL
                     RIERAKSLLARGSHNVTEACFEVGFSSLGSFSTLFAQRVGLSPSEYRRYARSSISVPD
                     TVRPLFVPSCFLAMLRGPADNSNFREASDTPPLVSSGQSSQTGR"
BASE COUNT          968 a         1727 c         1806 g         1011 t
ORIGIN      
        1 cttgaggacc tggggatcaa tgctcccgag ctccgcctcg ccggtggtgg aaccggagga
       61 agctcgggcg ggccctggcg gcaactgctg gctgacgtgc tcggccgccc gttgcggctc
      121 ttgcccgatg aggtctccgc cgtcgcctca gctcgtgggg ccgcgttcct ggccggcatc
      181 gcctccggcg tctaccaggc ggccggcgac acgctcgccc tcacccccga gccagaacat
      241 tccatactgc caggcgaggc tgactacgag gcagcctacg tccgctacac ggaactctat
      301 cccaggctgt accgttagag tggtccgtaa atgatcccca ccctgcgatt gtgagcgaag
      361 ccgctatacc gcaagatact ccttgctaca ctacgcttcg agatgatgag gtggcatagg
      421 acttgttgag cagagaatat gcgggctcca gaggggagac cggcgcgccc gagggcgccc
      481 gatggctcct tgcggaccgg gtgagccgcg tcctctcggg ggcgaacggt gtcactaacc
      541 cgaccgggcg cgtggtaagc ctcgccgtgc ctgtgggccg gattgacgcc ttcaggtggc
      601 tgagggagca gcgaaccttc ccgaagacgt actggtcggg gcgcgaggac gaggccggag
      661 tcgccgccgt gggagcggct gacttgcggg agtcgggcgt ttctgagggc cctggatctc
      721 tctccaggct actcgcggcg ttgcctgatg ccggggtttc ggaggcccgc tactacggcg
      781 gcatgcgctt cgatcccctg cggaagcccg acgaggaatg gagggcgttc ggcgcttacc
      841 gcttcgtcct accgcgcttc gaactgcacg caggggaagg cgagacggcg ctggtctgca
      901 acctgctgct cccgcgcgac gcggacaacg ccccggagat tctacgggag atagaagacc
      961 tcgccttgcc tccaggtact tctgaccctt ccctgcccat tccggtctcc cgtcaagaca
     1021 gtcccggcct gcgtggctgg agggagaacg tcgagcaggc catctccgcg ttcgcggagg
     1081 gaaggcttgg taaagtcgtg ctcgcccgca gggcggagtt cggtttcgat ggggaactgg
     1141 atcccatctt cctcctcgag agcctgaaag cggccacacc aggctgcttc catttctacg
     1201 ccgagcccaa agatggggcg gtgttcctcg gcgcctctcc cgagcggctg taccgtcgtg
     1261 acggacgctc cgtggtaagt gaggccgtgg caggaaccag gccccgcggc gcgtcgtcgg
     1321 cggacgacga tgggttgcgc gacgacctgc ttcacagcgc gaaagacctc tccgagcact
     1381 cctacgtgcg gataggtatc caggaggcgc tcgcgcctca ctgcaaggag ctggagatgg
     1441 accaggacat ctcggagatg aagctggcgc ggggaaggca cctgaggtcg aaggtcaggg
     1501 ggacgctaag ggagggcgtg acggacgccg ggctgctcga cgcgctgcac ccgacgcctg
     1561 ccgtcggcgg gcatccgcgg ggtgaggcga tggaggaaat aaacgctctc gaaccgttcg
     1621 acaggggctg gtacgccggt ccggtcggtt ggatcggggc cgaagcctct gagttcgccg
     1681 tgggcttacg ctcggggctc gtgcggggca ggacgctcgc gctcttctcg ggggcaggga
     1741 tcgtcgcagg ctccgcgccc gatgaggagt gggccgagat cgagcagaag atcggcgact
     1801 tcacagggat gttcgggctt gaacagggac acgccgcgag ctaaccgcct ctgggcgtac
     1861 ctgatcgtcg aagagcttct ccgctgcggc gtagaattct tctgcgtcgc cccaggctcg
     1921 cgctcgacac cactcgttgc ggcgctcgcc gcgaacgaga aagcccgtag cctcatccac
     1981 ttcgacgaac gcggaaccgc cttcgctgcc ctcggttacg cacgcgccac gcggcgtcct
     2041 gcggcctgga tcacgacctc agggacggct gtcgccaacg gcctccctgc cgtcgtcgag
     2101 gccgcgactg acggcgtgcc catgatcctg cttaccgcag accgtccgcc tgagctccgc
     2161 cagaccggag ccaaccagac catcgaccag cccgacatct tcggcgacta cgtacgctgg
     2221 cgcttcgatc tcccagcccc ggaccccgtc atcgaccccg ccatggtgct gacgaccgtg
     2281 gatcaagccg tctatcgagc catgcgaaca ccgcaaggcc ccgtccacct gaacctgatg
     2341 ttccgcgaac cttttctccc tgaggatgaa gatagtccat tcgcgccatc ctcgtggaca
     2401 aagagcggtg aaccgtacac ccgctacgcc gcgacgaaac ccaccgtgga ccagacggag
     2461 ctggatcttc tctgggaagc gatccgtctt acgaaacggg gcctcgtcgt cgcgggccgt
     2521 ctggcctccc gcaagcaggg cgaggccgtg atgcggctcg ccgaaactct aggctggcca
     2581 ctcctccctg acatcggttc ccagatccgc ctcggagctg actcggaggg cttcgtggcc
     2641 cactacgacg cgctcttatc cagtgactct ttcgccggag cgcacgcgcc tgaggccgtg
     2701 gtccacgtcg gaggaagggc gctctccaag cggcttgagc agttcctcgc ccgcagccgt
     2761 cccgatcctt acgtcgtcgt gcgagagaac ccgttccggc tcgacccgtc ccaccgcgta
     2821 acccacagcg tcgaggcgga cgtcgtggat ttctgcgccg cgctggcccg gtccgctacc
     2881 agagacccgg ctgcggtgga cgctacctgg gcggcgggct ggcaggaggc gtcagaggag
     2941 acgggtcacc atctggacga gatattctcc gggacccagg agccggacga gccgcaagtt
     3001 gccagagccg tatctcgaaa tatacctctg gatcatggtc tcatagtcgc gagtagcatg
     3061 cccgtacggg atctggacac ttacgccgcg accgacggtg cacccgttcc cgtcgcagcc
     3121 aacaggggcg cgagcggcat cgacggcacc gtcgccaccg cggcaggctt cgcgcatggc
     3181 tccggcagac ccgtcacgct cctgatcggg gacctcgccc tcctgcacga catgaactcc
     3241 ctcgccatgc tgagtgacct tcacgtcgtc gtcgtggtgc tcaacaacga cggcggcggc
     3301 atcttctcgt ttctccccgt agccagccag aaagagttct tcgagccgta cttcggcacg
     3361 ccgcaggggg tgggattcga gcacgcggct gcgatgttcg gcctcggcta cgagcgtccg
     3421 ggcacgatgg atgagttcgt cgagtcgtat cgtgtggcct gctctcgcgg gtactcgacc
     3481 ttgatcgaag ttcataccga ccgtgaagag aatgtggctt tgcaccgcag gatcctcgaa
     3541 gagatcgtcg cccggacaga gaaggggtga taggcgcgtg gcgcacggca gggtgacgga
     3601 gacgataccc gctccggcgc aggaggtctt cgaaggtgcg aaagtggctt cgagccctga
     3661 agaagtactt cgaactcctc cccgaccaca cacgggatgc gacgccagaa taggctgaac
     3721 ttcgaggtca ctggtgacag cgggtctccc gctgtcctct tcctgcacgg cttcatgggc
     3781 tcgtctgccg actggcgaga gataacgacg gctctggagg tcaggtccct ttgcatcgcc
     3841 gtagacctgc ctggacacgg cgcttcgctg ggaatggggc ccgaagcgta caccatcgat
     3901 ggagcggcgc gggctgtgat ctgtactctg gacgagctcg gcatcgagcg tcccgtgata
     3961 gccggctact cgatgggcgg gcggctggcg ctgtatctgg ccctgcgtta cccggaacgg
     4021 tgttcgggac tcttcctcga atccacctct cccggtcttg agcgggcgga ggagcggacc
     4081 gcgcgccgcg aggccgatga ggagaaggcg aagcgcctcg aatcccgaga cttcgaggaa
     4141 tttctggagg actggtatcg ccagccgctc ttcgcgtctc tggcgagcga cgagaagcta
     4201 ctcaggcgga ccatcgaggc gcgcctccgc aacgatccgg cggagctggc gcgttcgctt
     4261 aggaggatgg ggacagggag ccagccatcg ctgtggggag agttggaacg tctagccatc
     4321 cctgcgcttt ccgtcgcggg ggtgcttgat gagaagtacg cggggatctc atcgcgcatg
     4381 gcgggtctca gcccgtggat ggagtccgta gtcatccccg aaacgggaca cacggtgcat
     4441 gctgaggctc ccgcggtgta tgtctcactg ctcttacgct ttatggagga ctaactcttg
     4501 ggctgggtga ggctgaacca gttccctgag ttgtctttca cgatggcttc ggtgccgtag
     4561 aactgttcct ttggtggcga tacgaactcg acgccttgct tgctcaggcg ctcgtaatcg
     4621 ccgtggatgt cgtcggtctc gaagacaccg attccgaagg cgcctctggc gaccaatgct
     4681 ttcatctctt ctgcggtgtc ggcgtcgaac atcgggctcg aggatacctt ggccaggatc
     4741 acttctaggt cgggctgtcc cttggggttc aacgtcagcc agcgaaaact cgagtcgtcc
     4801 atggtcatgt cggtattgac ctcgaagccc aggatgtcac ggtagaattt cagggcctca
     4861 tcctggtcga tcacgtagac tgtcgagtgg ctctggcgtt ggatcacggc ttacctccct
     4921 gtctgactgc tctggcctga agatactagc ggaggtgtat ctgaggcttc tcgaaaattg
     4981 ctgttatccg ctggcccgcg caacatggcc agaaagcagg agggaacgaa gagcggcctg
     5041 accgtatccg gcacggagat actcgatcgg gcgtagcgcc tgtactcgga tggggataga
     5101 ccaacgcgct gggcgaacag ggtactgaag ctgcccaggc tggagaagcc cacctcgaaa
     5161 cacgcctcgg tgacgttatg gctccctctc gccaacaacg acttcgccct ctctatgcgc
     5221 aacctggtca ggaattcgtg cggcgtttct ccgtagactc ccttgtagac gcgcaggaag
     5281 tggtaggggg agaggtttgc ctgcgccgcg acgtcaggga gagagactgg acgccggaaa
     5341 gcgtaacgca tgaagtcgcg ggcatctctc aaacctctgt acgtctgggg gtgcatcctg
     5401 aagcaagagt acagtcggca tcaccggaat caaagatgat ctttgaccgc agcatgggga
     5461 gcgcgccata ggtctatcat gaggcgtggt tgagccggag gtcgagagat ga
//
LOCUS       DATAIR010000031         5784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_139295, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000031 DATAIR010000000
VERSION     DATAIR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139295"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VHM69_00820"
     CDS             complement(<1..1700)
                     /locus_tag="VHM69_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX2738958.1"
                     /translation="MPEPRSLSNLVVIGSSAGGVEALSLLLSTLPVDFPAPVIVAQHL
                     DPARESHLGEILGRKSTLPVRTVTDHEQLESGVVFVVPANRHVNVTGTEIDLRVDTHG
                     RPKPSIDLLLGSAAEVVGERLIAVILTGTGSDGAAGAGVVKKAGGTVIIQNPRTAKYP
                     GMPQSLAPNTVDIIAELDAIGEIIHGLLTSYPVPEEPEEQVALNALLEEVRERNGIDF
                     SGYKTPTIMRRLQRRIVATDSASIEGYADYVREHPEEFQHLVSSFLITVTEFFRDAEL
                     FEIIKDETVPQLIEEARNQGNQLRFWSAGCATGEEAYSLAILVAEALGDELGRFNVRI
                     FATDLDEEAIAFARNGIYAHSALANTPEALIDRYFVKENGNYQVNKLVRSMVIYGQHD
                     LGKRSPFPRIDMVLCRNVLIYFTSDLQKRALQLFAYSLRNDGRLVLGKAESTGALSEY
                     YVPENKHQKIYRRRGDRFLMPPALLESQGAAPPKWEPSSQRERRNLGLTPREQEQRRE
                     DKIINRFLLRLPIGVVVVDRRYDIQLINNTARRLLDIHSPATGEDLIHLTHGAGYATL
                     R"
     gene            complement(1767..2486)
                     /locus_tag="VHM69_00825"
     CDS             complement(1767..2486)
                     /locus_tag="VHM69_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2738959.1"
                     /translation="MDPEKEREDGRLSNDKEQLLSRVNFLQPIPPEELRDLANRTVEV
                     RLDTGQNLFTPRYANTMLFVVLEGRVRIYDIMAGREFTFLVAGTGEFFGDMALGDGQA
                     LGSYAQALEPSYICVMRQHVFEALVRRCPEVGLIMMRLLNYRLGQFGNRMLDVGLKEV
                     LPRMAGLIIYLAENEGVLTNGQMRITTRYTHQQLGTMIGANREAVTNALISLRGSGAI
                     KIVHRHIYIADVQALKEIAGD"
     gene            2892..3257
                     /locus_tag="VHM69_00830"
     CDS             2892..3257
                     /locus_tag="VHM69_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738960.1"
                     /translation="MVLRGGNEVVGTTLKSYEAVMDTLAAVQKQNMMLGVGWIAGSFR
                     MLQMQAEFNMRMAETVAGRADGVEQSPPIDGYDRLSVEEISPRLEQLDARGIEELKVY
                     EKRHKNRGMLVEKLDRALV"
     gene            3292..3537
                     /locus_tag="VHM69_00835"
     CDS             3292..3537
                     /locus_tag="VHM69_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738961.1"
                     /translation="MRSEDVYIGVRVRVASYGEPDLRGLDGTIAQVYGDPDYRAAEVR
                     LENGRSELFWYHELERTPARGGGVGGEASSGIGRASV"
     gene            3609..3905
                     /locus_tag="VHM69_00840"
     CDS             3609..3905
                     /locus_tag="VHM69_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738962.1"
                     /translation="MSVFTTRLQGGERALPVFGHSEEASDFLLFAGLEGGWEIRETSI
                     GELLSLLYCFCKDVQRVLLDPLPGIDVSLLWDLVDMRRQRFVEFLLSTTEPLAR"
     gene            complement(3922..4263)
                     /locus_tag="VHM69_00845"
     CDS             complement(3922..4263)
                     /locus_tag="VHM69_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738963.1"
                     /translation="MSSTSVRTSDPILIGVIVLTIGTALIHLYLGLQGFPLFILNGLG
                     YLTLLAALTLPIPRISDYRSLTRWVLAGYAALTVLLWILFGARNFIGYTDKIIELALI
                     SLLLLDARRQR"
     gene            complement(4291..5499)
                     /locus_tag="VHM69_00850"
     CDS             complement(4291..5499)
                     /locus_tag="VHM69_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2738964.1"
                     /translation="MAGWRSPLMFLFVTVFVDMIGYGIVVPLLPFYAGVYASGAVLVG
                     LLGSVYAAMQFAGGPFLGGLSDRHGRRPVLLLCLLGASLAYLLLGLARTLAVLVLAVV
                     LAGVTSGTLATSQAYIADSTTKEERARGLGMIGAAFGLGLMAGPALGGLLSLHSLSAP
                     ALFASALALANFFFGYVTLPESHPPHLRKAVPLLRLNPISQLGRILKMRNVRALLVAV
                     LLLNLAFAGLVSNFPLFSHVRFGWGTTSNAFFFAFVGVCAVVTQGLLIGRLQPRFGES
                     RLLLGGLALVALNLALVAMVPSGVFLYPVVGLLALGTGLAIPSLTAIISNRTPAGTQG
                     RLMGGLQAILSLAMILGPTLAGLAFDYLGVPAPYLIGGALAVLALIFSGADLMPGRRS
                     GTLRRAGSSG"
     gene            complement(5502..>5784)
                     /locus_tag="VHM69_00855"
     CDS             complement(5502..>5784)
                     /locus_tag="VHM69_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738965.1"
                     /translation="DLFARLTPGLAVVPVVVSGVISPSALRIPLTRLRRRRRDREWLA
                     ASLQMLVPALRNVDVWIEFGHPIYAEAGNAVGYEVIEETKRLMERREAA"
BASE COUNT         1115 a         1652 c         1908 g         1109 t
ORIGIN      
        1 cgcagcgtcg cgtacccggc gccgtgggtg aggtggatca gatcctcccc cgtcgccgga
       61 ctgtggatgt cgagcaggcg gcgggccgtg ttattaatca actggatatc gtagcgccgg
      121 tcgaccacca ccacgccaat gggtagcctg agaaggaagc ggttgatgat cttgtcctcc
      181 cgccgctgct cctgttccct cggtgtgagt cccaggttcc tacgctctct ttgagacgag
      241 ggttcccact tcggtggggc tgccccttgg ctctcgagga gggcgggcgg catcaagaag
      301 cggtcgccgc ggcggcggta gatcttttgg tgcttgttct ctgggacgta gtattcactc
      361 agggcgcctg ttgactcggc cttgccaagc accaggcgac cgtcgttcct caatgagtac
      421 gcgaagagtt gcagcgcccg cttctgtaga tcggaggtga agtagatcag cacgttgcgg
      481 cagaggacca tgtctatcct ggggaagggt gaccgcttgc cgagatcgtg ctgtccgtag
      541 ataaccatcg agcgcaccag cttgtttacc tggtagttgc cattttcttt gacgaagtag
      601 cggtctatta gcgcctctgg cgtgttggcg agcgccgaat gcgcgtagat accgttcctg
      661 gcgaaggcga tggcttcctc atcgaggtcc gtggcgaaga tgcgcacgtt gaaccgtccc
      721 aactcgtcgc ccagagcctc ggctaccagg atagccagcg agtaggcctc ctcgcccgtc
      781 gcgcagcccg ccgaccagaa gcgcaactgg ttgccctggt tcctggcctc ctctataagc
      841 tgcggcacgg tctcatcttt gattatttcg aacagctcgg cgtcgcggaa gaactccgtc
      901 acggtgatca ggaacgagct cacgaggtgc tggaactcct cgggatgttc cctcacgtag
      961 tcagcgtacc cctctatgct cgcagagtcg gtcgcgacga tccgcctctg caggcggcgc
     1021 atgatcgtgg gggtcttgta gccggagaaa tctatgccgt tgcgctctcg cacctcctcg
     1081 agcagcgcgt tcagcgccac ctgctcttcc ggctcctccg ggacggggta actcgtaagc
     1141 aggccgtgga ttatctctcc tattgcgtca agctccgcga tgatgtcaac ggtgtttggg
     1201 gcgagcgact ggggcatccc ggggtatttt gcggtcctgg ggttctggat tattacagtg
     1261 ccgcctgctt tcttgaccac cccggcccct gctgcgccgt ccgagccggt gcccgtgagg
     1321 atgacggcta tcaaacgctc accaacgacc tcggccgcgc tgccgagcag cagatctatg
     1381 gagggcttgg gccgtccgtg ggtgtctacc ctgaggtcga tctcggtgcc cgtaacgttg
     1441 acgtggcgat tggcaggcac cacgaagacc acgcctgact caagctgctc gtggtccgta
     1501 accgtgcgga cgggaagggt gctcttgcgc ccgaggatct ctccgagatg gctctcgcgg
     1561 gccgggtcga gatgctgggc gacgatgaca ggagcgggaa agtctaccgg aagagtcgag
     1621 aggagcaggg acagggcctc gaccccgccc gcggacgagc ctatcaccac gagattggaa
     1681 agcgacctgg gctcaggcac aatagcacct ccgccggtcc tggaagggct gggcgcgaaa
     1741 agtatatcgg tcgggtctaa acatgatcaa tctcccgcaa tctccttcag tgcctggaca
     1801 tcagcgatgt atatgtgacg gtgcactatc tttatcgccc ccgatcctcg taggctgatg
     1861 agggcgttgg ttacggcttc gcggttggcc ccgatcatcg tgcccagttg ctggtgggtg
     1921 taacgcgtcg taatcctcat ttgcccgttg gtgagcactc cctcgttttc ggcgaggtag
     1981 atgatgagac ctgccatgcg gggtaagacc tctttcaagc ccacgtccag catcctgttg
     2041 ccgaattggc ccagccggta gttgagaagc ctcatcatta tgaggcccac ctcgggacat
     2101 cttctcacca gtgcctcgaa cacatgctgg cgcatgacgc agatgtacga cggttccagt
     2161 gcctgtgcgt aggatcccag agcctggcca tccccgagtg ccatatcacc gaagaactct
     2221 ccagtcccgg ccaccagaaa ggtaaactcg cgtcccgcca ttatatcgta gatccttacc
     2281 cgcccttcga gaaccacgaa gagcatggtg ttcgcgtacc gcggcgtgaa taggttctgg
     2341 cccgtgtcca ggcgaacctc cacagtccta ttggcaagat ctcttagctc ctccggcggt
     2401 attggctgga gaaaatttac tctggagaga agctgctcct tatcatttga caacctgcca
     2461 tcttcgcgct ccttttctgg atccaaccga tagcatcctt gcactcaaaa cccaccgcgc
     2521 gacagtcgga ttctacaata gttgagacga atagtaacta taagtgagac gaatacgaga
     2581 atgtcggtag gccgcacacc cgtaccatac tggtaggccc ttcaggtatc gatggcccaa
     2641 tatgttgttg tatttagcct atggaagatt tgtgaattgt attacagggt accgatattt
     2701 attagccttc cgtcgaagat atagcgcgtt ggccctcttg gcaggagtcc agtacgccgg
     2761 gaagcgtctg ggcgagggaa aggaggagag ggccgcaaat gtgccggtcg gatacggggc
     2821 ggtatcctgc tcattacccc ctcctgggcg ggatcccggc ctgtgttcga tggctttgag
     2881 agaaggaggg aatggtgctt cgcggcggaa atgaagtagt cggtacgacc ctgaaatcct
     2941 acgaggcggt catggacact ctggcggccg tccagaagca gaacatgatg cttggtgtgg
     3001 gttggatcgc gggctcgttc cgcatgctgc agatgcaggc ggagttcaac atgcgcatgg
     3061 cggagacggt agcggggagg gcggacggag tagaacagag cccaccgata gacggctacg
     3121 acaggctcag cgtcgaggag ataagcccca ggctggagca actcgacgcc cgtggaatcg
     3181 aggagctcaa ggtctacgaa aagcggcaca agaaccgcgg gatgctcgtt gagaagttgg
     3241 atcgtgctct ggtctgaccg ttctgagcag ccggagtcag gaggtggctc gatgcgttca
     3301 gaggatgttt atatcggcgt ccgggtgagg gtggcgtctt acggcgaacc ggatctgcga
     3361 gggctggacg gcacgatagc gcaggtgtac ggagatcccg actacagggc cgctgaggtg
     3421 cggttggaga acggacgatc cgagctgttc tggtatcacg aattggagag gactcccgca
     3481 cgcggcggag gggtcggtgg tgaagctagt tccggcatcg ggcgcgccag cgtctgatag
     3541 agatgggcca cgcggaacac acgacgcttt catcgtactg gttgatcaca aagtgtagtc
     3601 gtggccacat gagcgtgttt accaccaggc tccagggtgg cgagcgggcg cttccggtct
     3661 tcggccatag cgaggaagcc tcggattttc tgctgttcgc gggtctggag ggcggatggg
     3721 agatcaggga gaccagcatc ggggagttgc tctcgttgct ctattgcttc tgcaaggatg
     3781 ttcagcgcgt gttgctcgac cccttacccg gaatcgacgt ctcgttgctg tgggatctcg
     3841 ttgacatgag acgccagcgc ttcgtggagt ttctcctgag cacgacagag cctcttgccc
     3901 ggtagggcga gcgccgcttt cctatctctg gcggcgggca tcgagcagga gcagagagat
     3961 cagggccagc tcgatgatct tgtccgtgta gcctatgaag tttcgcgccc cgaacagtat
     4021 ccacaggagg accgtcagcg cagcgtaccc ggcgagcacc cagcgtgtga ggctcctgta
     4081 gtccgatatc cgtggtatcg gcagggtcag ggccgcaagg agcgtgaggt atcctagtcc
     4141 gttcaggatg aacagaggga agccctggag gccgaggtag aggtggatca gggccgtccc
     4201 gatggtcagg actatcaccc cgatcaggat cgggtcgctt gtccggacgc ttgtggagga
     4261 catgttcttg ctcctttccg gttcatgtac tcatcccgat gaccccgcgc gtcgtagagt
     4321 cccggacctc cgacctggca tcaggtcagc gcctgagaag atcagggcga gcacggcgag
     4381 tgccccgccg atgaggtaag gcgcggggac gccgaggtag tcgaaggcga gtccggcgag
     4441 cgtcgggccg aggatcatgg cgaggctgag gatggcctgc agacctccca tgagcctgcc
     4501 ctgcgttcct gcaggggtcc tgtttgagat gatggccgtc aggctcggga tggcgagccc
     4561 cgtccccaat gccagaagtc cgacgacggg gtagaggaat actcctgaag ggaccatcgc
     4621 gacgagagcg aggttgagcg cgacgagggc gagtccgccc aggaggagcc tgctctcccc
     4681 gaagcgcggc tggaggcgcc cgatcagtag cccctgcgtg acgaccgcgc acactccgac
     4741 gaaggcgaag aagaaggcgt ttgacgtcgt cccccacccg aacctgacgt ggctgaagag
     4801 cgggaagtta ctgacgagcc ccgcgaaggc caggttgagc agcagcacgg ccacgaggag
     4861 tgcccgaacg tttctcatct tcagtatgcg acccagttgg gagatggggt tgaggcggag
     4921 aagaggaacc gccttgcgca ggtgtggcgg gtgcgactcg ggaagggtca cgtagccgaa
     4981 gaagaagttg gcgagggcga gggctgaggc gaagagggca ggggcactca atgagtggag
     5041 gctcagaagg ccgccgaggg ctggtcccgc catgagcccg agcccgaagg cggccccgat
     5101 catgccgagc cccctcgccc tttcttcctt cgtagtgctg tccgcgatat acgcctggga
     5161 tgtcgccagc gtgccgctcg tgacgccggc caggacgacg gcgaggacga ggacggcgag
     5221 cgtgcgcgcg aggccgagca gcaggtaggc gagcgatgcc ccgagcaggc aaagcaggag
     5281 gacgggacgc ctcccgtggc ggtcggagag cccgccgagg aacggtccgc cagcgaactg
     5341 cattgcggcg tagacggagc cgagcaggcc gacgagcacg gcgccagacg cgtatacgcc
     5401 ggcgtagaag ggcagcaaag gaaccacgat accgtacccg atcatatcca cgaacaccgt
     5461 gacgaaaagg aacatgagcg gcgaccgcca cccggccaac tttacgccgc ctccctgcgc
     5521 tccataagtc tctttgtctc ctcgatcacc tcgtagccga cggcgtttcc cgcttcggcg
     5581 tagatggggt ggccgaactc gatccacacg tccacgttgc gcagggccgg gacgagcatc
     5641 tgcagtgagg cggcgagcca ctcgcggtcg cggcggcggc ggcgcaggcg ggtcagaggt
     5701 atgcgcaggg cggaaggtga gatcacaccg ctgaccacga cggggacgac ggcgagtccc
     5761 ggcgtgaggc gcgcgaagag gtcg
//
LOCUS       DATAIR010000032         5115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_417209, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000032 DATAIR010000000
VERSION     DATAIR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417209"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VHM69_00860"
     CDS             <1..1393
                     /locus_tag="VHM69_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2738966.1"
                     /translation="MWRMYFREVLNEDLGCASYVVADGGEAAIIDPKWEIEEYLRIAG
                     ENGFGISYILETHNHADHLSGKGRLAEATGAKIYISGDAGVAYEHEPIEDGEVILVGD
                     TRIRAIATPGHRPEHLSFAVQDTSRGDEAWALVTGDSLFVGDLARPDLAVEAEEGARG
                     LFRSMRKFSGFADFVEVRPGHIGGSLCGGANMSRKPDSTMGFERRFNRYLRLENEEEF
                     VATLTAEQTPQPPNFERIVELNRGPLLTEAAPLAPLLPRRVKELVGEGAVLIDGRDQR
                     EFDAAHVPGSINVTTNQTGVGTRAAWMVDPESEVIVAADGDEEARRMARMLEAVGFRR
                     IRGYLVGGMSGWRASGLQADTIPALDIPGLAQKIRNKEIVVLDVRSSAEWEAGHVGGS
                     IHVPYQRLRDGIPDEIRDSDVGPLAVICGSGVRSALAASLLKRSGVRNVEHVAEGGVP
                     MLAGYGIELVESD"
     gene            1419..2183
                     /locus_tag="VHM69_00865"
     CDS             1419..2183
                     /locus_tag="VHM69_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2738967.1"
                     /translation="MTLALLAVLMGLSLGLLGGGGSILAVPILVYAAGLGAKEAIAAS
                     LVVVGLTSLFGAAGHWREGRVRIRVALAFGPVAAVGAYLGARLANYLSGAVQLSLFAV
                     VMLIAAYFMWRNGESGKDGKPDLTDGSVGRLLLRCAAPGMAVGVLTGLVGVGGGFLVV
                     PVFVLLGEVPVQAAVGTSLLVIAMNSFAGFAGYLGEVEIPWGLVSLFGALAVVGTFAG
                     AYLVRFVPQHALKKAFATFLVVMAVFILYENAGGVL"
     gene            complement(2303..2713)
                     /locus_tag="VHM69_00870"
     CDS             complement(2303..2713)
                     /locus_tag="VHM69_00870"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF006155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX2738968.1"
                     /translation="MRLDQGLVDAAVSLAENRWPEGEGGAAAMYLEDGRVVTSVFAES
                     PNGAAELCHETGAICEAHKLNLAVTASVCVSRETERDPFVILTPCGICQERLAFWGRD
                     VEVAIPDPEDATKWQTRRLYEVQPYYWRNIFDTE"
     gene            2921..4144
                     /gene="glyA"
                     /locus_tag="VHM69_00875"
     CDS             2921..4144
                     /gene="glyA"
                     /locus_tag="VHM69_00875"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX2738969.1"
                     /translation="MIGPYTEVVDSEVQEALDGELERQRTTIELIASENFTSPAVLAA
                     QGSVLTNKYAEGYPGKRYYGGCEWVDKAEQLAIDRAKRLFGAEHVNVQPHSGSQANEE
                     AYAVLVEPGEKVLAMDLAHGGHLTHGAKFNFSGRTYDFVHYGVGEDGYIDYDQVRDIA
                     LRERPKMVLAGASAYPRVFDFAKFREISDEVGAFFLTDMAHIAGLVAADVHPSPIPYC
                     EVVTTTTHKTLRGARGGMILCREEFAKKVNSRVFPGMQGGPLMHAIAGKAVAFGEALQ
                     PEFKDYARNIVANCKALAEGLLEGDIDLVSGGTDNHLILVDLKEKGVTGKELEESLES
                     IGVTCNKNMVPNDPEPPTVTSGVRLGTAAMTTRGMGEDEMREIASIIVATTRGELDGL
                     RDRVRTLTDAFPLYE"
     gene            4137..4784
                     /gene="upp"
                     /locus_tag="VHM69_00880"
     CDS             4137..4784
                     /gene="upp"
                     /locus_tag="VHM69_00880"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX2738970.1"
                     /translation="MSEIRPESKNLHVVDGPLSRHKLAVLRDKRTHTPDFRQAMKELA
                     LILVAEATRNMPTRPVQIRTPLTETEVEEISGPVCLVPVLRAGLGMLDGALALLPTAT
                     VGFMGLQRDEETALPVEYYVNRPRNLEDYLVLVLDPMLATGGSLSATIDKLKEEGATW
                     ISCLHAVAAVPGVERVTSEHPDVTFYTAAIDPELDDRSFIVPGLGDAGDRLYGTL"
     gene            4771..>5115
                     /locus_tag="VHM69_00885"
     CDS             4771..>5115
                     /locus_tag="VHM69_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX2738971.1"
                     /translation="MEPFEDRAVDTSSSHETLKKVRPSWDEYFMRIAHEVATRSTCPR
                     LAVGAVIVRDKRILTTGYNGSPSGMPHCDEVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVSVTGASIY"
BASE COUNT          947 a         1608 c         1662 g          898 t
ORIGIN      
        1 gatgtggagg atgtactttc gagaagttct gaacgaggat ctgggctgtg cttcctatgt
       61 tgtcgcggac ggcggcgagg cggccatcat agacccgaaa tgggagatag aggagtattt
      121 gcggatagcc ggggagaacg gttttgggat atcgtacatc ctcgagaccc acaaccatgc
      181 tgaccacctc tccgggaaag gtcgtcttgc agaggcaacg ggcgcgaaga tctacatctc
      241 cggggacgca ggggtcgctt acgagcacga gcccatcgag gatggagagg tgatcttggt
      301 cggcgacacg cgcatccgag caatagcgac gccgggtcac aggcccgagc acctctcttt
      361 cgcagtacag gacaccagca ggggcgatga ggcctgggcg ctcgtcacgg gcgactcgct
      421 tttcgtcggc gacctggccc gtcccgacct cgccgtcgag gccgaagaag gcgcgcgggg
      481 tctattccgc tcgatgcgaa agttcagcgg gtttgcggac ttcgtcgagg tcaggccggg
      541 gcacatcggg ggatccctgt gcggcggcgc taacatgagc cgcaagccgg actcgacgat
      601 gggattcgag cggcgcttca accgctacct gcgcctcgaa aacgaggaag agttcgtcgc
      661 gaccctgacc gcggagcaga cgccccagcc accgaacttc gagcgcatcg tggagctcaa
      721 ccgcggcccg ctactgacag aggccgcgcc gctcgcgccg ctgctgcccc ggcgcgtaaa
      781 ggagctcgtc ggggagggag ccgtcctaat cgacggcagg gaccagcggg agttcgacgc
      841 agcccacgtt cctggctcca tcaacgtcac cacgaatcag actggggttg gcacgcgggc
      901 cgcgtggatg gtcgatcccg agagcgaggt aatagtggcc gccgacggtg acgaagaggc
      961 caggcgcatg gcccgcatgc tcgaggccgt gggtttccgc cgcattcggg gatatctcgt
     1021 cggcgggatg tccggctggc gagcgagcgg tctccaggcc gataccatcc cggctctcga
     1081 cataccaggc ctcgcacaga aaataaggaa caaggaaata gtcgtcctcg acgtcaggag
     1141 ctcggccgaa tgggaggctg gacacgttgg gggttcgatc cacgtcccct accagaggct
     1201 gcgcgatgga attcccgatg agatccgcga ctcagatgtc gggccgctcg ctgtgatctg
     1261 cggcagcggc gtccgcagcg ccctcgccgc gtcgctcctc aagaggtccg gtgtgcgaaa
     1321 cgtcgagcac gtcgcggaag gcggcgtccc gatgctggcc gggtatggga tcgaactcgt
     1381 cgagagcgac tgaggccccg agtcagaatt cggttcagat gactctggcg ctacttgccg
     1441 tgctcatggg cctctctctt ggcctgctgg gcggcggagg atcgatactc gccgtaccga
     1501 tcctcgtcta cgctgctggc cttggagcga aggaggccat cgcggcgagc ctcgtcgtgg
     1561 tggggctgac gagcctcttc ggggccgccg ggcactggcg ggaaggccgt gtgaggatac
     1621 gcgtggccct tgccttcggc cccgtcgcgg cggtgggggc gtacctgggg gcgcgcctcg
     1681 caaactacct gagcggcgcg gtccagctct ctctgttcgc cgtggtaatg cttatcgcag
     1741 cctacttcat gtggcgcaac ggcgagtctg gtaaggatgg caagccggat ctcaccgacg
     1801 ggtcagtggg gcgactgctc ctgcggtgcg ctgcgccggg catggcggta ggcgttctca
     1861 ccggcctggt cggcgtgggg ggcgggttcc tggtcgtgcc agtcttcgtg ctcctcggtg
     1921 aggttcctgt gcaggcggcg gttgggactt cgctgctagt catcgcgatg aactccttcg
     1981 caggtttcgc agggtatctt ggcgaggtgg agatcccgtg ggggctcgtg tcgctcttcg
     2041 gtgccctggc ggtagtaggg accttcgccg gcgcctacct cgtacggttc gtcccccagc
     2101 acgcgctcaa gaaggctttc gcaacgttcc tggtcgtgat ggccgtcttc atcctctacg
     2161 aaaatgcggg tggggtactt tgagaaggtg tgatctaccc gaccgagacg atacgtagcg
     2221 gcaacaggtt tgcaccgctt cggcacgcta tcccaaacag agccctctac gcccatgttg
     2281 tcggactgac catcgtcttt cactattccg tgtcgaagat gtttcgccag tagtagggtt
     2341 gtacttcgta caatcgtctt gtttgccact tcgtcgcgtc ttcggggtcc ggaatcgcaa
     2401 cctccacatc gcggccccag aacgccagcc gctcctggca gatgccgcag ggggtcagga
     2461 tcacgaacgg atctcgctcc gtctcgcgcg agacgcatac ggaggcggtc actgcgaggt
     2521 tcagcttgtg ggcctcgcaa attgctccgg tctcgtgaca cagctcagcg gccccgttcg
     2581 ggctctcggc gaacacgctg gtaacgacgc gaccgtcctc gaggtacatg gccgccgcgc
     2641 cgccttcacc ctcgggccag cgattctcgg ctagactgac tgcagcatct acgagtccct
     2701 ggtccagacg cataccaacc tcccttactg aacgtccgag ttgtgcggtt caatatactc
     2761 gtcgcggggt ttgagggctc tagcctgaac gtctatactc cgtacagttg cagggctggt
     2821 agggaggaag cggggagatc aaagccccac agcatcgctc aaccaccact ttccccggcg
     2881 cggcgccggg cacttctcta acccgaagcg gaggtagatc ttgatcggac cgtacaccga
     2941 ggtcgtcgat tccgaggtcc aggaggcgct cgacggcgaa ctggaacgcc agcgcacgac
     3001 aatagaactt atagcgagtg agaacttcac ttctcctgcg gtgctcgctg cacagggctc
     3061 ggtccttact aacaagtacg cggagggtta ccccgggaag cgctactacg gcggttgcga
     3121 gtgggtggac aaggccgagc agctcgccat agatcgggcg aaacggctct tcggggccga
     3181 acacgtcaac gtccaaccgc actctggtag ccaggccaat gaagaggcgt atgccgtcct
     3241 ggtcgaaccg ggggaaaaag ttctcgcgat ggacctggcg cacggaggac acctcacgca
     3301 cggggccaaa tttaacttct cgggacgaac ctacgacttc gtccactacg gcgtcggcga
     3361 ggacggctac atcgactacg atcaggttcg cgacatcgcg ctccgcgaga ggccgaagat
     3421 ggtcctggcc ggtgcaagcg cctaccctcg cgtcttcgac ttcgcgaagt tccgcgagat
     3481 ctccgacgag gtcggagctt tcttcctgac ggatatggcc cacatcgcgg gcctcgtcgc
     3541 cgcagacgtc cacccgtccc cgatccctta ctgcgaggtc gtgacaacga cgacacacaa
     3601 gacgctgcgg ggagccagag gcggcatgat cctctgccgc gaagagtttg ccaagaaggt
     3661 gaacagccgc gtcttcccag gaatgcaggg cggcccgctg atgcacgcca tcgccggcaa
     3721 ggcagtcgcc ttcggcgagg cgctgcaacc cgagttcaag gactacgccc gcaatatcgt
     3781 cgcgaactgc aaggcgctgg ccgagggact gttggaaggc gatatcgacc tcgtttcggg
     3841 cgggacggac aaccacctga tcctcgtcga cctgaaggag aagggcgtca ccggcaagga
     3901 actggaagaa tccctggagt ccataggcgt cacttgcaac aagaacatgg tccccaacga
     3961 ccccgaaccc ccgaccgtga ccagcggcgt caggctcggg acggcggcga tgacgacgcg
     4021 tggcatgggc gaggacgaaa tgcgcgagat cgccagcatc atcgtggcga ccacgcgcgg
     4081 cgagctcgac ggtctgcgcg acagggtgag gaccctgacg gacgctttcc cgctgtatga
     4141 gtgagatccg gcccgaatcg aagaacctgc acgtcgtaga tgggcccctg agccgccaca
     4201 agctcgcggt cctgcgcgac aagcggacgc atacgccgga cttcaggcag gcgatgaaag
     4261 agctcgccct catcctcgtc gcggaagcca ccaggaacat gccaacaagg ccggttcaga
     4321 tccgtacccc cctcacggag acggaggtag aagagatctc cggtcccgtc tgcctggtcc
     4381 ccgtgctgcg cgccggcctc ggtatgctcg acggggcact cgccctgctg ccgactgcga
     4441 cggtcggctt tatgggcctc cagcgcgacg aggagacggc cctgcccgtc gaatattacg
     4501 tgaaccgccc gcgcaacctg gaggattacc tggtactagt cctcgacccg atgctcgcga
     4561 cgggggggag cctctcggcc acgatagaca agctgaagga ggagggggcg acctggatct
     4621 cctgtctcca cgccgtcgcc gccgtgcccg gcgtcgagcg cgtcacgagt gagcaccccg
     4681 acgttacctt ctacaccgca gctatcgacc ccgagctgga cgaccgctcg tttatcgtcc
     4741 ccggtctggg cgacgctgga gacaggctct atggaaccct ttgaagaccg cgcggtagac
     4801 acttcgagct cccacgagac cctcaagaag gtccgacctt cctgggacga gtacttcatg
     4861 cggatagcgc acgaggtcgc gactcgctcg acgtgccccc gcctcgccgt cggagcggtg
     4921 atcgtacgcg acaagcgcat cctcaccacg ggctacaacg gcagcccgtc cggcatgccc
     4981 cactgcgacg aagtaggatg ccttatacga atcgtggacg gccgcgagag ctgccagcgt
     5041 acgctgcacg ccgagcagaa cgccatcatc caggccgcct accacggcgt gtcggtgacg
     5101 ggggcttcga tctac
//
LOCUS       DATAIR010000033        10453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_371059, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000033 DATAIR010000000
VERSION     DATAIR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371059"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VHM69_00890"
     CDS             <1..594
                     /locus_tag="VHM69_00890"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX2738972.1"
                     /translation="TQRPSVDVITGMIKANVPSRIAFAVSSQVDSRVILDTPGAEALL
                     GMGDMLYKPVSAARPSRVQGAFISEAEVDRVVSATVEAASGRVEARFVEEVTEPRSVK
                     EESDEPEDDLLPEAANFVVSTQQASVSAVQRRFRVGYSRAGRIIDALERKGVVGPYEG
                     SKSRSVVATTMDLESLFGDGEGSAARTDGKADLAEGW"
     gene            620..1528
                     /locus_tag="VHM69_00895"
     CDS             620..1528
                     /locus_tag="VHM69_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX2738973.1"
                     /translation="MDDWQHTPHGDGTSGEAKIGRILEQKRKEMGLSLDEVEQATKIR
                     KRYLTGLEREDYAMLPDAVYARGFLKTYANYLGLDGEALSQQLKSRRKPRRERGIDYN
                     NEPRTSFEQPLITPSGLTGTEKRKISTSAIVTLLVALLALAAIIGTLYFVGRGVQASR
                     SQDDNPPPSSGNPPHQEEQQKKVASKDETPEGGQKKEDDSEQNVGNVEETTGAEQSAP
                     PDTLQVLVNVTERPSWLLIRTDGSRAFEQVAEPGFSETFEAERKLYIKSGDAGAVRVE
                     INGQDAGVLGQTGEVVAKSYSLKSAS"
     gene            1642..2691
                     /gene="recA"
                     /locus_tag="VHM69_00900"
     CDS             1642..2691
                     /gene="recA"
                     /locus_tag="VHM69_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2738974.1"
                     /translation="MDKSKAIDAAVSQIERQFGKGSIMRMGDEERPKIACIPTGALAL
                     DLALGIGGVPRGRIVEIFGPESSGKTTLALHIIAEAQKAGGLAAFIDAEHALDPTYAQ
                     AIGVDLENLYFSQPDNGEQALEIADTLIRSGALDVVVIDSVAALVPRAEIEGEMGDSH
                     VGLQARLMSQALRKLSGSLSRSKTTAIFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIGALKVGSDAVGNQTRVKVVKNKVAPPFKTVEFDVMYGEGISREGSLLDVGI
                     EHGVVQKSGAWFAYGDERLGQGRENAKKFLKENYEVRERILDEVYEKLGLNEPVDVAD
                     EVAEPSAQEPARQVL"
     gene            2703..3323
                     /locus_tag="VHM69_00905"
     CDS             2703..3323
                     /locus_tag="VHM69_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX2738975.1"
                     /translation="MPVITGVRERRGKARISVDGEFWAELDAGVAAECGLREGAAFSG
                     AELDRARVAGERPVAMGRALNLLGYRARSEAEIRERLRRYGYLDQSIESVVLRLQELG
                     YVDDTEFARLKAREKARRYGPRRVTAELMHSGVGEVLAHEVVQEEFAGRSEVGEARSA
                     AARRYNERGSDAEARRVYGFLVRRGYSAEVCAQVAREYREPPEARG"
     gene            3383..4927
                     /gene="rny"
                     /locus_tag="VHM69_00910"
     CDS             3383..4927
                     /gene="rny"
                     /locus_tag="VHM69_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX2738976.1"
                     /translation="MNILLLVLGLVAGLAVGAALGYFVLKSRLDSRQVQARADARSII
                     EDANREAETKRREADLGAKEAALKIKDDAEAEVRTRRAEIARVEERLDNRDTSLDRRE
                     GELDERRRRLTETEEALQRKKDELREREAEHLRVLEEISGLTRADAERRLFSGLESEL
                     ESRLGQMVRDKTLEAEERADAEARRLISTTMERLASDLTSESTVKAVSLPSDDMKGRV
                     IGREGRNIRAFEASTGVDVIIDDTPETVVISSFDPVRREVARVSMGRLVQDGRIHPGR
                     IEQIVAKARKDVEKEMKSAGRQALYDAKISGGMHNDLMRLLGALKYRTSYGQNVLAHS
                     VEVANLCGMMAQELGANVKVARRAALLHDVGKAIDHEVEGTHALIGGRFAKKSGESDD
                     VVRAISAHHHEIEMETVEDVLVAAADAVSAARPGARRETTETYIERLRSLEDIALGHR
                     GVDKAYAIQAGREIRVMVQPSEVDDRIAAKLAYDISKQIESDLEYPGQIKVTVIRESR
                     VSEVAR"
     gene            4932..5858
                     /locus_tag="VHM69_00915"
     CDS             4932..5858
                     /locus_tag="VHM69_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2738977.1"
                     /translation="MEGPYRDLGAGFARLTGVEGARRGPSRITHVEGALLELLRNARD
                     AGASNIYVSSTLKHRRYRTLTVIDDGHGIPESHRDLILEPGVTTRHLNPISDPDDPPH
                     GAGLSLYQIKALSLATKVLSPSSPTSIQAIFDTKTLPERTLQSTTRPSKSNLRTTLMT
                     FTAHTNARNNAQALNSYYGSPARILATLLHNHIIQPDKESARLQEVAEALGLVVSVRS
                     VQRILRGEIRPVESISGGGSGEGTRSRKGVRVVGEGPVLSLGEQETVRIADILRQIAG
                     AHYMAIEDMRLESRPGEISLRARVYEPEEEYE"
     gene            5851..7185
                     /gene="miaB"
                     /locus_tag="VHM69_00920"
     CDS             5851..7185
                     /gene="miaB"
                     /locus_tag="VHM69_00920"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2738978.1"
                     /translation="MSSERVTAAPIPLSRKPARRDGPDSGQRTACIRTFGCQMNVHDS
                     DRMRRMIQDAGYAEVQRYEEADLVILNTCYVRENAVDRVRGHLGELNRLRREGRVKKV
                     ALTGCIGAAEESAALERTYNIDLVLGTHNTYELAEFVGLPTMRETYTPELPGTEGEHN
                     SFITIMTGCNYQCSYCIVPTVRGRMVCRPMENVLEEVDRLVAGGTRYVTLLGQTVDAW
                     RYEGKRFCDLLEAVSERAPRVWFTTSHPSNMEDRTLRAIGERDTIVKRLHLPVQSGSD
                     RMLKVMHRGYKAERYRRKIEVFREAVPDGTLSTDIIIGHPGETEEDHEQTLELVEDCA
                     FDSAYIFKFSPRRGTEAAEMPGAVPDAVVQRRFLQVLRQVEKNAFARNQRKVGAVEDI
                     FIRHGSTEDGKAIGETWSGHAVHVGTGVAPGHYVKARIVSAGPHVLYARPSS"
     gene            7317..8042
                     /locus_tag="VHM69_00925"
     CDS             7317..8042
                     /locus_tag="VHM69_00925"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX2738979.1"
                     /translation="MQVYKEIPVITNQARVRPAELVGIVSVTEEWTVARHKARAEEVI
                     DADAPEHFVLDAGTGMYLNAILLNIPLAPKVSQELRRRAQETVEDELNPRRAARAREL
                     ELAGAEGRGSIWDGDLRYDTAIVYLRPDRAAIDAAIDERSRKIALDGLQEAAEIKALA
                     AVNPSVTDSVGVRELTEHLSGTLSLQQAEELIATRTRRLARRQMRWFDKLARTLDGRA
                     RITLVRNPDHHKLVHSMHGILRI"
     gene            8039..9313
                     /gene="hflX"
                     /locus_tag="VHM69_00930"
     CDS             8039..9313
                     /gene="hflX"
                     /locus_tag="VHM69_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2738980.1"
                     /translation="MNEERAILVGAGETRHMAELGRLASTLGIEVAAVLEQGRRDGTG
                     YIGRGKREELRELVADAEARFVISDDELTASQARVLEKACGASVVDRTELIIRIFEAH
                     ARDAASRLEVELADLEYRLPRVKGKNPELSRLGGGGVTTRGPGEQQLEYDRRVIRERI
                     ATINRKLKEEKAGRAVRGAKLKEAGPPTVALIGYTNAGKTTILNALSGARRSTRDRLF
                     ETLETTTRLVKGSGTDGTSGGARPDFVVTDTVGFIRKLPTQLVHSFASTLESARDADV
                     KVLCADASSEDLEEEITTVSQTLSETFAKENGAEWGTTIVCLNKVDLLDEARIQELRR
                     ELPDAVQISALAGCEALLEEIYSAIASERERMAVLIPHAEYAAASHLYGLAEIHAQEN
                     TPTGVWMDVSLPRSASTRYAPYRTSNNTPKRR"
     gene            9431..10093
                     /locus_tag="VHM69_00935"
     CDS             9431..10093
                     /locus_tag="VHM69_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2738981.1"
                     /translation="MNRPYVFINMVSSVDGNVTKEGKASGIGTAADRRVMRTLRSKAD
                     AVMIGAGTLRAEKLSLGLDAGDTGTSPLAVILSSTGDVPLESNLVRYGHQRILVLIAD
                     SADHAADTRLGRHAEIRRVRNTASGDVDLTSAIEILKTEFGVDRLLVEGGPTLNHALI
                     SESLADELFITLAPTLFGTGNADAPGVLGKPLDEPQILHLLSAYLADDELFLRYSLND
                     AA"
BASE COUNT         2209 a         3147 c         3400 g         1697 t
ORIGIN      
        1 acccagcggc ccagcgtcga cgtcataacg ggcatgatca aggccaacgt cccaagccgc
       61 atcgccttcg cagtctccag ccaggtggac agcagggtga tcctggacac ccctggggcg
      121 gaggccttgc tcggtatggg agacatgctc tacaagcccg tctccgcagc gaggccgtcg
      181 cgcgtgcagg gcgctttcat ctcggaggcc gaggtggacc gtgtcgtctc ggccaccgtc
      241 gaggcggctt cgggacgcgt cgaggcacgc ttcgtcgagg aagtcaccga gccgaggagc
      301 gtcaaggagg agagcgacga gcccgaggac gatctcctgc cagaggcggc caacttcgtc
      361 gtctccacgc agcaggcgtc ggtgagcgcg gtgcagcggc ggttcagggt cgggtactca
      421 cgggcgggca ggatcatcga cgcgctggag cgaaagggcg tcgttggccc gtacgagggt
      481 tccaagagcc gctccgtggt ggcgactacg atggatctcg agtccctgtt cggcgatggt
      541 gaaggctccg cagcccgcac tgacgggaag gcagatctgg ccgagggttg gtagcttttg
      601 tgataggctt ctaggcgcca tggacgactg gcagcacacc ccgcacggcg acggaacaag
      661 cggcgaggcc aagatcggtc gcatcctcga gcagaagcgg aaggagatgg ggctctccct
      721 cgacgaggtc gagcaggcca cgaagattcg caagcgctac cttacgggcc tcgagcgcga
      781 agactacgcc atgttgcccg acgcggtcta cgcaagaggc tttctcaaaa cctacgccaa
      841 ttacctcggc ctcgacggcg aggcgctctc gcagcaactc aagagccgca ggaaaccgcg
      901 gcgcgagcgc ggtatagact acaacaacga gccaagaacc tccttcgagc agcccctgat
      961 cacacccagc ggcctgacgg gtacggagaa gcggaagatc tccacttctg cgatagttac
     1021 cctgctagtc gccctgctcg cccttgccgc catcatagga acgctttact tcgtcgggcg
     1081 cggcgtacag gcatcccgct cacaggatga taacccgcca ccctccagcg ggaacccgcc
     1141 tcaccaggag gagcagcaga agaaggtagc ctccaaagac gagacccccg aaggcgggca
     1201 gaagaaagag gacgacagcg agcagaacgt cggcaacgtg gaggaaacta ccggagcaga
     1261 gcagagcgca cctcccgata cccttcaggt cctcgtcaac gtaacagaga ggccctcctg
     1321 gctactgatc cggaccgacg gaagcagggc tttcgagcag gtcgccgagc cggggttctc
     1381 agagactttc gaggccgagc ggaagctgta catcaagagt ggcgatgccg gtgcggtgag
     1441 ggtcgagatc aacggccagg acgccggggt gctcggacaa actggtgagg tggtcgccaa
     1501 gagctactcc ctcaagagcg cgagctgaac cgtttcacac agcataaacc cccttgggag
     1561 ccatcacccc ctacagtata atcaggcgca gataatgccg attacattcg gtgttggcac
     1621 actctcgttt tggaggatgc attggataaa agcaaggcca tagacgccgc agtctcccag
     1681 atagagcggc agttcgggaa gggttcgatc atgcgcatgg gcgacgagga gcggccgaag
     1741 atcgcctgca tcccgaccgg cgccctcgcc ctcgacctcg ctcttggcat cggcggcgtg
     1801 ccgcgcggcc ggatcgtcga gatcttcggt cccgagtcca gcggcaagac gaccctcgcc
     1861 ctgcacataa tcgcggaggc acaaaaggcc ggcggcctcg ccgccttcat cgacgccgag
     1921 cacgccctcg acccgaccta cgcccaggcc atcggcgtgg acctggaaaa cctctatttc
     1981 tcccagccgg acaacggcga gcaggccctg gagatagcgg acaccctcat ccgttccggt
     2041 gccctcgacg tcgtcgtcat agactccgtg gcggccctcg tccccagggc tgagatcgag
     2101 ggagagatgg gcgactctca tgtgggactt caagcccgtc tgatgagcca ggccctgcga
     2161 aagctctccg ggtctctcag ccgttctaag acgacggcta tcttcatcaa ccagctcagg
     2221 gagaagatag gggtcatgtt cggctcgccc gagactacgc caggcgggcg ggcgctgaag
     2281 ttctactcca gcgtgcgtct tgacatccgg cgcatcggtg ccctgaaggt cggcagcgat
     2341 gcggtcggta accagaccag ggtcaaggtc gtgaagaaca aagtagcgcc gcctttcaag
     2401 acggtcgagt tcgacgtgat gtacggagag gggatctccc gcgaaggcag cctgctcgac
     2461 gtcggcatcg agcacggtgt tgtacagaag agcggcgcct ggttcgccta cggcgatgag
     2521 cgcctagggc agggcaggga gaacgccaag aagttcctca aggagaacta cgaggtccgc
     2581 gagcgcatcc tcgacgaagt ctacgagaag ctcgggctga acgagccggt cgatgttgcc
     2641 gacgaggtgg cagagccttc cgcccaggag cctgcccgcc aggtgctcta gggcgacgcc
     2701 ccatgcccgt gataaccggc gtccgcgagc gtcgggggaa ggccagaatc tccgtcgacg
     2761 gagagttctg ggccgagctg gacgccgggg tggcggctga gtgtggtctc agggaagggg
     2821 ccgcgttttc gggcgcggag ctggatcggg cgcgggtcgc tggggagagg cccgtcgcta
     2881 tgggccgggc gctcaacctc ctcggctaca gggccaggtc cgaggccgag atccgcgagc
     2941 ggttgcgccg gtatgggtac ttagatcaga gcatagaaag cgtcgtcctt cggcttcagg
     3001 aactcggcta cgttgacgac accgagttcg cccgtctcaa ggcccgcgag aaggcgcgca
     3061 ggtacggtcc caggcgggtc acggcggagc taatgcacag tggcgtgggg gaggtccttg
     3121 cgcatgaggt agtccaggag gagttcgctg gccgttccga ggtgggggag gctcgatctg
     3181 ccgccgcgcg gcggtataat gagcgtgggt ccgacgccga agcgcgtagg gtctacggct
     3241 ttctggttcg ccggggttac tcggcggagg tatgtgccca ggttgcccgg gagtaccggg
     3301 agcctccgga ggccagggga tagtcgagcg agcttgatgc cgggccgccg ttcccgcggc
     3361 aggtccgctg gaaggacaac ctgtgaatat tttgttactg gtcctgggac tggtcgctgg
     3421 cctcgccgtc ggcgctgccc tgggctattt cgtgttgaag tcacgtctgg attcccgtca
     3481 ggtccaggcg cgcgctgatg ccaggtccat catcgaggat gctaaccgcg aggccgagac
     3541 gaagcgccgc gaagctgacc tgggagcaaa agaggccgca ctcaagatca aagacgacgc
     3601 cgaagccgag gtgcgtacca ggcgggcgga gatcgcgcgc gtcgaagagc gtctcgacaa
     3661 cagggacacc tctctggacc ggcgcgaggg cgagctcgac gagcgcaggc gccgcctcac
     3721 cgagacggaa gaggcgcttc agcgcaagaa agatgagctc agggagaggg aagcggagca
     3781 cctcagggtg ctcgaggaga tctccggcct gacaagggca gacgcggaga ggaggctctt
     3841 ctccggcctc gagtcagagc ttgagagccg gcttgggcag atggtgcgtg acaagaccct
     3901 ggaggccgag gagagggcag acgcggaagc tagacgtctg atctccacga ccatggaacg
     3961 cctcgcctcg gacctgacct cggagtctac ggtcaaggct gtgtcgctgc cttctgacga
     4021 catgaaagga cgcgtcatag gccgcgaagg gaggaacata agagccttcg aggcatccac
     4081 aggggtcgac gttatcatcg acgacactcc cgagacggtg gtgatctcca gcttcgatcc
     4141 ggtccggcga gaggtggccc gcgtttcgat gggacggctg gtgcaggacg ggcgtatcca
     4201 ccctggccgc atcgagcaga tcgtcgcgaa ggcccgcaag gacgtcgaga aggagatgaa
     4261 gtccgcggga cgccaggccc tctatgacgc caagatctcc ggcggcatgc acaacgacct
     4321 catgcggctt ctcggcgcgc tgaagtatcg cacttcctac ggccagaacg tcctcgccca
     4381 ctctgtcgaa gtggccaatc tctgcggcat gatggcccag gaacttggcg cgaacgtgaa
     4441 ggtcgctcgc agggcggcgc tgcttcacga cgtaggcaag gcgatcgacc acgaggtcga
     4501 ggggacgcac gcgctgatcg gcggccgctt cgccaagaag agcggcgagt cggacgacgt
     4561 ggtgcgtgcg atcagcgccc accaccacga gatcgagatg gagacggtgg aggacgtcct
     4621 cgtggccgcc gccgacgcag tctccgcggc ccgccccggc gcaaggcgcg agacgacgga
     4681 gacgtacatc gagcggctgc gctcactgga ggacatcgcc ctcggccaca gaggcgttga
     4741 taaagcatat gcgatccagg ccggtcgcga gatacgggtc atggtccagc cctcggaggt
     4801 ggacgatcgc atcgccgcga agctcgccta cgatatctct aaacagatag agtccgacct
     4861 cgaatacccg ggccagatca aagtgaccgt catccgcgag agccgcgtca gcgaggtcgc
     4921 caggtaatct ggtggaaggc ccctacaggg acctcggcgc aggcttcgcc cgcctgaccg
     4981 gcgtcgaggg ggcccgccgc ggaccttctc gtatcaccca cgtggagggc gccctcctgg
     5041 agcttctgcg caacgcccgc gatgcaggag catcaaacat ctacgtctca tctaccctga
     5101 agcaccgcag gtaccgtacc ctcaccgtca tcgacgacgg ccacggcatc cccgaatcgc
     5161 acagggacct gatccttgaa cccggcgtga ccacccgcca cctgaacccg atctccgacc
     5221 ccgacgaccc tcctcacggc gcaggcctct ctctctacca gataaaggcg ctctccctcg
     5281 cgacgaaagt cctctcccca tcttcgccaa cctcgataca ggccatcttc gatactaaaa
     5341 ccctccccga acgtaccctg cagtccacaa cccgaccctc gaagagcaac ctccgtacca
     5401 ccctcatgac tttcacagcc catactaacg cccggaacaa cgcccaggcc ctcaactcct
     5461 actacggatc acctgcccga atcctcgcca cccttctaca taaccacata atacaaccag
     5521 ataaggagag cgctcgctta caagaggtgg ctgaggcgct aggtctcgtt gtatccgtga
     5581 ggagcgtaca gaggattttg cgtggagaga tacgtcccgt cgagagtatt tcgggagggg
     5641 gtagcggtga aggaacgagg agcaggaagg gagtacgggt ggtgggggag gggcccgtgt
     5701 tgtcgcttgg ggagcaggaa accgtccgca tcgcggatat actaaggcaa atagcggggg
     5761 cccattacat ggcgatcgag gacatgaggc tcgagagtcg gccgggggag atctcgctca
     5821 gagcccgcgt ctacgagccg gaggaagagt atgagtagtg agagagtaac ggctgcgccc
     5881 ataccgctct cccgaaagcc ggcgaggcgg gacggacccg acagtggcca aaggaccgcc
     5941 tgcataagga ccttcgggtg ccagatgaac gtgcacgact cggatcgcat gcggcgaatg
     6001 atccaggacg caggctacgc tgaagtccag cgttacgaag aagccgacct cgtaatcctg
     6061 aacacctgct acgtacgcga gaacgccgtt gaccgcgtac gcgggcacct cggagagctc
     6121 aaccgcctgc gccgcgaggg tcgcgtcaag aaggtcgccc tcacaggctg catcggcgcc
     6181 gccgaggagt ccgcggccct cgagcggacc tacaacatag acctcgtcct gggtacccat
     6241 aatacttacg aattggctga gttcgtggga ctaccgacga tgcgggagac gtacacaccc
     6301 gagttgcctg gcacggaagg cgaacacaac tccttcatca ccataatgac gggatgcaac
     6361 taccagtgca gctactgcat cgtaccgacg gtaaggggac gcatggtgtg ccgtccgatg
     6421 gagaacgttc tggaggaggt agataggctt gtcgccggcg gcaccaggta tgtgacgcta
     6481 cttggccaga ccgtggatgc ctggagatac gaaggcaagc ggttctgtga tctcctcgaa
     6541 gccgtctcgg agagggcgcc gcgcgtatgg ttcacgacga gccacccatc gaacatggag
     6601 gaccgcacgc taagggctat cggtgagagg gataccatcg taaagcggct ccacttgccg
     6661 gtccagtcgg gatccgacag gatgctcaag gtcatgcacc gcggctacaa agcggagcgc
     6721 tacaggcgca agattgaagt cttcagggag gctgtgccgg acggcacgct aagcactgat
     6781 atcatcatcg gccatcccgg cgagacggag gaggaccacg agcagacgct cgagctggtc
     6841 gaggactgcg ccttcgactc ggcatacatc ttcaagttct ccccgaggcg gggcacggag
     6901 gcagccgaga tgcccggggc tgtccctgac gccgtcgtgc agcgacgctt cctccaggtg
     6961 ctcaggcagg tagagaagaa cgccttcgcg aggaaccagc gtaaagtcgg ggccgtggag
     7021 gacatcttca tacgccacgg tagcacggaa gacggcaagg ccatcgggga gacgtggagc
     7081 ggacacgctg ttcacgtcgg caccggcgtc gcgcccggac actacgtaaa ggccaggata
     7141 gtgtccgccg gcccccacgt actctacgcc aggccatcaa gctgagcgaa agctcgaaga
     7201 cggcgctcgt cgtctgcgga ccgacggcga gcggcaagag cgacctgtcc gatgccctcg
     7261 ctgagcggct cacaaccgta gatggccgcc acgtgccgac gctggccgtc gattccatgc
     7321 aggtatacaa agagataccc gtgataacca accaggcccg agtgcgcccc gccgagctgg
     7381 tcggcatcgt ctccgtgaca gaggaatgga ccgttgccag acacaaagcg cgcgctgagg
     7441 aggttatcga cgcagacgca ccggagcact tcgtccttga tgccgggacg ggcatgtact
     7501 tgaacgcgat cctgctgaac atcccgctcg cgccgaaggt ctcacaagag ctgcgccgca
     7561 gggcgcagga gaccgtagag gacgaactca acccgcgacg ggccgcccgc gcaagagaac
     7621 tggagcttgc aggagccgag ggtcgaggtt cgatctggga cggagacctt cgctacgata
     7681 cggccatcgt ctaccttcgc cccgatagag ccgcgatcga cgctgcaatc gacgagaggt
     7741 cccgtaagat agccttggac ggtctacaag aggccgcaga gataaaggcc ctggccgcgg
     7801 ttaacccttc cgtaacggac tccgtcggcg tgcgcgagct caccgaacac ctctcgggaa
     7861 ccctttcgct acagcaggca gaggagctca tagccacgcg taccagacga ctcgcccggc
     7921 gccagatgcg ctggttcgat aaactcgctc gtaccctcga tggccgcgcc agaataacct
     7981 tagtgaggaa tccggatcat cacaagctag tgcatagtat gcatggtata ctaaggatat
     8041 gaacgaagag agagcgatac tagtaggcgc gggggagacg cgtcacatgg cggagttggg
     8101 gcggttggcg agcacgttgg ggatcgaggt cgccgccgta ctggagcaag gccgcaggga
     8161 cggcacgggc tacatcgggc gtggcaagcg tgaagaattg cgggaattgg tagcggatgc
     8221 tgaggcgcgg ttcgtgatct cggacgacga gttgacggcg tcgcaggcga gggtgctcga
     8281 gaaggcgtgt ggcgcgtctg tcgttgacag gacggagttg atcatccgca tcttcgaggc
     8341 gcatgccagg gatgctgcca gccgtctcga ggtcgagctc gcggatctag agtaccgctt
     8401 gccgagggtc aagggcaaga accccgagtt gagccgcctc ggaggtggag gagttacgac
     8461 gagggggccg ggagagcagc agctcgaata cgacaggcgc gtcatccggg agcgcattgc
     8521 gactattaac cgcaagctca aggaagagaa ggccggaagg gccgtcaggg gcgcgaagct
     8581 caaggaggca ggaccgccga ctgttgcgct cataggctat acgaacgcgg gcaagaccac
     8641 gatactcaac gcgctcagcg gggcgaggag gtccaccagg gaccgtctct tcgagacact
     8701 ggagactacg acgcgcctgg tcaaagggag cggtaccgac gggaccagcg gcggcgcgcg
     8761 gcccgacttc gtcgtgacgg ataccgtggg cttcataagg aagttgccga cgcagctcgt
     8821 gcactccttc gcctcgacgc tggagtcggc gcgcgacgcc gacgtcaaag tactttgcgc
     8881 cgacgcctcc agcgaggacc tcgaagagga gatcacgacc gtcagccaga cgctctcgga
     8941 gaccttcgcg aaggagaacg gggctgagtg ggggacgacc atagtgtgtt tgaacaaggt
     9001 agacctcctc gatgaggcac gtatccagga actccgcagg gaactgcccg acgccgttca
     9061 gatcagcgcg ctcgccggtt gtgaggctct gctggaggag atctacagcg caatagcttc
     9121 tgagagggag aggatggctg tgctgatccc tcacgccgag tacgccgcag cgagccacct
     9181 ttacggcctc gctgagatac acgcccagga gaacacacca accggagtgt ggatggacgt
     9241 gagcctccct cgttccgcct ccacacgata cgctccctac agaacttcca ataatactcc
     9301 caagcgcaga taggcggagc gaggcttgtc cgggaagatg ctaaggctgt tccctatgct
     9361 agccagggaa cagtcaaaga gtatagaaat ctacgagagc cttgagttgc ccaggtcggg
     9421 tcgtggcggt atgaacaggc cgtacgtatt tatcaacatg gtcagttccg tagatgggaa
     9481 tgtgaccaag gaaggaaagg cgtccggcat cggcaccgct gcggacagac gtgttatgcg
     9541 cacgttacgc tccaaggctg acgccgtaat gatcggggcg ggtacgctgc gggcggagaa
     9601 gctctcgctc ggcctcgacg caggagatac gggcacgagc ccgctagccg tcatactatc
     9661 gtctacaggt gatgtgcctc tggagagcaa cctggtacgc tacggtcacc agaggatcct
     9721 ggtcctcatc gccgatagtg ccgatcatgc cgcagatact cgccttggtc gccacgcgga
     9781 gatccggcgc gtacggaata ccgcgtctgg agatgtggat ctcaccagtg ccatcgagat
     9841 tctcaagacc gagttcggcg tagaccgtct gctcgtcgag ggcggtccga cattgaacca
     9901 tgccctgatc tctgagtccc tcgctgatga gctctttatc accctggctc cgacgctctt
     9961 tggaacaggc aatgcagacg cgccgggcgt actcggaaaa ccgctagacg agccgcagat
    10021 tctccatctc ctctcagcat acctcgcaga cgatgaactc ttcctcaggt attccctcaa
    10081 tgatgctgcc tgacggtgga gctgtactaa cgattgtcct gtcctggagg acacttgccc
    10141 agcgaactgc cgagaattgt aaacaagcga atatcatcca cgattccgta tatccatcca
    10201 acaccacaat atgtagttcc ctgcggtcaa gggctgtagg ttgttagaag ctatttttga
    10261 gcctatagga tcccggctat gcaggatccg gtatatgaac ttcggagaat taaccttcca
    10321 cgctgtgagt atcctggaga atggctgtgg gcgatgcctc ggagcggcct gaccctcctt
    10381 ccacgtgggc cccaagggtg ccttgcaagt gactcgtcgg ccggacgcct tggcgtcgcc
    10441 cgcaggccgc ccg
//
LOCUS       DATAIR010000034         8022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94124, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000034 DATAIR010000000
VERSION     DATAIR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94124"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VHM69_00940"
     CDS             complement(<1..660)
                     /locus_tag="VHM69_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2738982.1"
                     /translation="MTERNAGSRGSTGRKYNRRDFLKMGGAGLAGAALLGAAGCGGGG
                     SGSGNLIFSMGPDDEQGTMATLIKRFNEQSDFKVEHRVMPSDTGQYFDKLRTQFQAQA
                     GGIDVIGGDVIWPAQLAANGWILDISDKFPEGEQGKFLDGPMQSVTYEGKVYGVPWYT
                     DAGMLYYRADLLEESGYSEPPKTWDELKEMAAKVQKDAGIKYGFVFQGDAYEGGVCNG
                     LE"
     gene            complement(773..2005)
                     /gene="ugpC"
                     /locus_tag="VHM69_00945"
     CDS             complement(773..2005)
                     /gene="ugpC"
                     /locus_tag="VHM69_00945"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2738983.1"
                     /translation="MAEVTLEEVTKVFGEDVVAVNKMNLDIPDGQFVVFVGPSGCGKS
                     TALRMIAGLEDISGGKVFIGDQVVNDLPPRQRDIAMVFQNYALYPHMNVRENMGFALK
                     LRKMDKSEISRRVDEAARILSIERFLDRKPKALSGGQRQRVALGRAIVREPKAFLMDE
                     PLSNLDAKLRVQMRTEIAKLHNRIGVTTIYVTHDQTEAMTMADRIVVLKDGEVQQIAT
                     PQIMYDQPDNVFVAGFIGSPAMNFILAHMEKQNGGYDIAFGRTRLHLTQEEIARARER
                     GYDPEAFTGKEVILGIRPEHIEDAGTEAAEAIGGSQGENTMEVDPQVIESMGSEKYLY
                     FEVPREQAAHLESLSEMTDDEGAEEGDGEAPAEESGDMMVARVAAESTAKEGQKMRLV
                     IDATKVQLFDPETEKAIF"
     gene            complement(2099..3280)
                     /locus_tag="VHM69_00950"
     CDS             complement(2099..3280)
                     /locus_tag="VHM69_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2738984.1"
                     /translation="MIRARSRSEFRLWDVLRVAILFVSIIFVGCAGPSGDGASGGAQD
                     QTSSSAREAGEKASLLSHMSVRDMVGQMFVISVGGTGPDYYIEKMVRERNIGGVILFS
                     HNMKSEEQVKSLTGSLQKLSIRTVPAVPLFVAVDQEGGDIASAPWVAPQPAAAEIGSR
                     GDPDEARKVAAQIGGQLLRAGVNTDFAPVVDTGFGAAIGNRSYGEDPELVARMGAAAV
                     DGFKEAGVVSAAKHFPNHGPATSDSHVSLPVVRHDQKTIESYDLPPFRAAVDAGVPMV
                     MVGHLVYPEIDPEHPASLSKDAISMLREDLGFNGVVITDDLAMAGATGGGPPARAAVE
                     AVGAGADLLIISSPPREQADAYDAVVAAVRSGELSRTQIRESVGRILAVKEKYHLRGG
                     R"
     gene            3353..4711
                     /locus_tag="VHM69_00955"
     CDS             3353..4711
                     /locus_tag="VHM69_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2738985.1"
                     /translation="MIELFYEAGTLVATGASSGLPEQFVWDGRTHQWRAPASAYRETV
                     LGLRESGYPHEDRAAAFEKVPLESRVAMEPRPYQKEALGAWRQAGLRGVVVLPTGAGK
                     TAVAVSAMERVGRSALVVVPTLALLKQWYSILHDAFGESLTIGLLGGGYHEITPLTVT
                     TYDSAYIHAERYGDRFALVVYDEVHHLPAPKNAIIAKMLLAPYSLGLTATPERPDGGH
                     ELLPDLVGGVVYRRSPEDLAGTYLAPFELVRIPIELTAHERFEYAQAEAVYRDFLDKH
                     RLPMRSPEDWQRFIMVASTSHSGGREALLAARRRREIREGAMRKGATLETLLRRHWDD
                     RVIVFTKSVEEVYALSRRFLIPGVTHETPARERKEILDRFREGRYRAVIASDVLNEGV
                     DVPDAGVAVILAGSASRREYVQRLGRILRPREGKRAVLYELVTAQTGEEFTARRRKEA
                     FL"
     gene            4711..5970
                     /locus_tag="VHM69_00960"
     CDS             4711..5970
                     /locus_tag="VHM69_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX2738986.1"
                     /translation="MLRSEHVIARLNRGRLVPHRLSPDDQRVTDVAGELCDLYAAHLG
                     LPRSSLERELTVREEDLGPGLDARRGFKIVRALAKLLEERANWTEPTEADPYTLRTRI
                     FELAAALPEPPGTEPGLLEAPTREDVLSRVAVETGVDEPAALMYADRQGAQLLDAFDR
                     PSPEELIRRYNVAQVQGVLYSARDLVVDLGERADARLVFHYVKLLGLIYRLEPLSHGH
                     RLHLDGPLSIFGGTRKYGLRLAKFLPGLLMTSPWNLSANVEWKGRDTVLELDSKKSGL
                     ESHYLGPKVEREADDTREAFIRAWERTKDTGGWELERGGDVLPFPQKKAALVPDFTLR
                     HADTGEVVHLEILGFWSERNLVERVALIREAGEQGHRVLLAASERLGASQEALSGAVE
                     GGVVPFKDRLAAKAVLAALAERDATLA"
     gene            complement(5962..7311)
                     /locus_tag="VHM69_00965"
     CDS             complement(5962..7311)
                     /locus_tag="VHM69_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2738987.1"
                     /translation="MAPTRSINTLVISAILAFVALALFAAGTGGAQSAGPVVDDRLQE
                     TLAGAEAGTQVRAILTYDEKPSATQVQNVKRTGVDVHRFDVLPMLGVRGTKSQVGRLL
                     DMWAPVSVYADRKLDYLLHESVRTIGANRVRSGLGYDGTGVGVAVIDSGVDGTNSDVS
                     YPGRLVQNVKIAGDAFFTGNTVVLENQKNTDTSSGHGTHVAGTVGGSGANSGGYYTGV
                     APGSDLVGIGTGDTLLILYALEGFDYALQNQEKYDIRVISNSWGTSGKFDRNDPINRA
                     SKLAHDRGMTVTFAAGNEGPGKDTLNPYSVAPWVIGVAAGKKDGKTLASFSSRGVLGD
                     NLYHPDITAPGVNIVSARASTGATINGLAAADDATYIPRTEWLPYYTTASGTSMATPH
                     VSGTVALMEQANPALGPDAIERVITATATPMPGYAEFEVGAGYLDAYEAVSRARALR"
     gene            7411..7848
                     /locus_tag="VHM69_00970"
     CDS             7411..7848
                     /locus_tag="VHM69_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738988.1"
                     /translation="MERNTGSLIGLTVACTISAVIFGFGAEMFSWRSAYEGLGRESLI
                     QTTRLVVYVTLGVLLAFRGGWHGVLAAIAMAFVATSVEWALYPFSYAWASIEDPAGYA
                     DTFGGVQRPDYAFWTTYDIIGVGISAALAQGLRTMAQVNPRDL"
     gene            complement(7829..>8022)
                     /locus_tag="VHM69_00975"
     CDS             complement(7829..>8022)
                     /locus_tag="VHM69_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738989.1"
                     /translation="WRPTECKRCRGRGRLVTGVEIEDSVEGVLPRSRYDFNALGEVYL
                     LDFDEDEELEVVRPRDPWG"
BASE COUNT         1326 a         2747 c         2420 g         1529 t
ORIGIN      
        1 ctcaagaccg ttgcacaccc caccctcgta ggcgtccccc tggaagacga agccgtattt
       61 gatgccggcg tccttctgga ccttggcggc catctccttg agctcgtccc aggtcttggg
      121 gggttctgag taccctgatt cctcgagcag gtcggccctg tagtagagca tcccggcgtc
      181 cgtgtaccag gggaccccgt agaccttacc ctcgtaggtg accgactgca tgggaccgtc
      241 gaggaacttc ccctgctcgc cctcgggaaa cttgtcggag atgtccagga tccagccgtt
      301 ggccgcgagc tgggcaggcc agatcacatc cccgccgatc acatctatgc cgccggcttg
      361 ggcctggaac tgggtcctga gcttgtcgaa gtactgcccc gtatctgaag gcatcaccct
      421 gtgttcgacc ttgaaatcgc tctgctcgtt gaaccgcttg atcagcgtcg ccatggtgcc
      481 ctgctcgtcg tcgggcccca tgctgaagat caggttccca gaaccgctgc cgccgccacc
      541 gcaaccggcc gcccccaata gggccgcccc ggccaggccg gcgccgccca tcttcaggaa
      601 gtccctccgg ttgtactttc tcccggtgga gcccctggat cccgcgtttc tctccgtcat
      661 gctcaagtag ccctttctat gacgacataa cgtgttgagt tactctgctc ctacgaagcc
      721 gggtgcgcct cgccggtact ccggcgtgat ccgcacccgg cttccgacct ctctagaaga
      781 tcgccttctc cgtctcgggg tcgaagagtt gcaccttggt cgcgtcgatg accaggcgca
      841 tcttctgccc ctccttcgcc gtgctctcgg ccgcaaccct cgcgaccatc atgtcgcctg
      901 actcctcggc aggggcttct ccgtcccctt cctcggcacc ctcatcgtcc gtcatctcgg
      961 agaggctctc caggtgggcg gcctgctccc ttggcacctc gaagtagagg tacttctccg
     1021 agcccatgct ctctatgacc tgcggatcga cctccatcgt gttctcacct tgtgagccgc
     1081 cgatagcctc cgccgcctcg gtccccgcgt cctcgatgtg ctctggcctg atacccagga
     1141 tcacttcctt gccggtgaag gcttctgggt cgtagcctct ctccctggcc ctggcgatct
     1201 cttcctgggt caggtgcaga cgtgtcctgc cgaaggcgat gtcgtagccg ccgttctgct
     1261 tctccatgtg tgccaggatg aagttcatgg ccgggctccc gataaagccg gccacgaaga
     1321 cgttgtcagg ctggtcgtac atgatctggg gggtcgctat ctgctgcacc tcgccgtcct
     1381 tgaggacgac gatccggtcg gccatcgtca tggcctcggt ctggtcgtgg gtgacgtaga
     1441 tggtcgtgac gccgatcctg ttgtgcagct tggcgatctc cgtcctcatc tgcaccctga
     1501 gcttggcgtc gaggttggac agaggctcgt ccatgaggaa cgctttgggc tcgcggacta
     1561 tggcccgtcc cagagccacc cgctgcctct ggccgcctga gagcgccttc ggcttcctgt
     1621 ccaggaaacg ctcgatcgag agtatcctcg cggcctcgtc cacgcggcga gagatctcgg
     1681 acttgtccat ctttctgagc ttgagggcaa agcccatgtt ctcgcgcacg ttcatgtgcg
     1741 gatacagtgc gtagttctgg aagaccatcg ctatgtcgcg ctggcgcggc ggaaggtcgt
     1801 tgacgacctg atctcctatg aatactttgc ccccgcttat gtcctcgagg ccggcgatca
     1861 tcctgagcgc ggtggacttg ccacagcccg atggacctac gaagacgacg aactgcccgt
     1921 ctggtatgtc caggttcatc ttgtttacgg cgaccacgtc ctcgccgaag actttggtaa
     1981 cctcctcgag ggttacttcg gccatcccaa ttcctccttc catctatccc aaacggctcc
     2041 gtccaggatt cccttgttgc ctttgttata ccccttactg gctacattct acctaatgct
     2101 agcgcccccc gcgcaagtga tatttctcct tgaccgcgag tatgcgcccg acggactccc
     2161 ggatctgtgt gcgagataac tctcctgacc gcacggcggc cacaacggcg tcgtacgcgt
     2221 cggcctgctc tcgcggcggg ctggagatta tgaggaggtc ggccccagcc ccaacagcct
     2281 cgacagcagc ccttgcggga ggccctccgc cggtcgcccc cgccatcgcc aggtcgtcgg
     2341 tgatgacgac cccgttgaag ccgaggtcct cacgcaacat gcttatggcg tccttcgaga
     2401 ggctcgccgg atgctcgggg tcgatctcag gatagacgag atgccctacc atcaccatcg
     2461 gcaccccagc atcgaccgcg gccctgaacg ggggcaggtc gtaggactcg atagtcttct
     2521 ggtcgtgccg tacgacgggc aggctcacgt gagagtctga ggtcgctggc ccgtggttcg
     2581 ggaagtgctt ggccgccgag acgacgccgg cctccttgaa gccatcgacg gcggccgccc
     2641 ccatcctggc taccagctcg gggtcctccc cgtaggagcg gttgccgata gcagcaccga
     2701 agcccgtgtc cactacggga gcgaagtccg tgttgacgcc agcgcgcagt agctggccgc
     2761 caatctgggc agcgacctta cgggcctcgt cagggtcgcc gcggctcccg atctcggccg
     2821 ccgcaggctg cggcgctacc cagggggccg atgcgatgtc tcccccctcc tggtcgacgg
     2881 cgacgaagag aggcaccgcc ggcactgttc ttatggagag cttctggagc gagccggtca
     2941 gagacttcac ctgctcctcg ctcttcatgt tgtggctgaa gaggatcaca ccgccgatgt
     3001 tccgctcccg caccatcttc tcgatgtagt aatcgggccc ggtgccgccg acgctgatta
     3061 cgaacatctg cccaaccatg tccctgacgc tcatgtgaga cagcaaggat gctttctcgc
     3121 cagcctcgcg cgcactggat gaagtctggt cctgcgcacc acccgaagcg ccatccccgg
     3181 aagggcctgc gcatccaacg aaaattatcg acacgaacag gatcgccacg cggaggacat
     3241 cccacagcct gaactccgac cggctacgtg cccgtatcaa ggtctacttt cgatcacggc
     3301 gcggccagtt taccgctgag agtggagaag ttctcggtta tgcttgtaag gtgtgatcga
     3361 gctattctac gaagccggca ccctcgtcgc gaccggggcc tcctcaggcc tgcccgagca
     3421 attcgtctgg gacggaagga cccaccagtg gcgggcaccg gcgagcgcct accgtgagac
     3481 cgtactcggg ctgcgcgaat cgggatatcc gcacgaggac agggccgccg ccttcgagaa
     3541 ggtcccgctg gagtcgcggg tggcgatgga gccccgccct taccagaaag aggccctcgg
     3601 cgcctggcgt caggcgggac tccgcggcgt tgtggtcctg ccgaccggcg ccgggaagac
     3661 ggccgtcgcg gtgagcgcga tggagcgcgt cgggcggagc gcgctcgtcg tcgttcctac
     3721 actggcgctg ttgaagcagt ggtactccat cctccatgac gccttcggcg aatctctcac
     3781 catagggctg ctcggcggcg gataccatga gatcacacct ctcacggtaa cgacctacga
     3841 ctcggcctac atccacgccg agcgatacgg cgaccgcttc gcgctcgttg tctatgacga
     3901 ggtccaccac ctgcctgccc cgaagaatgc gatcatcgcg aagatgctcc tggcgcccta
     3961 ttccctcggc ctcacggcca cacccgagag acccgacgga ggacacgaac tgctccccga
     4021 cctcgtcgga ggggtcgtct accgacgctc tcccgaggac ctcgctggca cctacctcgc
     4081 ccccttcgag cttgtccgta tacccatcga gctcacggcc cacgagcgct tcgagtacgc
     4141 ccaggccgag gccgtctaca gggacttcct cgacaaacat cgtctgccga tgcgttctcc
     4201 ggaggactgg cagcgcttta tcatggtcgc gtcaacgagc cattcagggg ggagggaggc
     4261 gctactcgcg gcgcggaggc ggcgtgagat cagggagggc gccatgagga agggcgcgac
     4321 gctggagacg cttctcagga ggcactggga cgaccgcgtc atagtgttca ccaagagcgt
     4381 cgaagaagtc tacgcgctca gccgcaggtt cctgatcccc ggcgtgaccc acgagacgcc
     4441 tgcccgtgaa cgcaaggaga tactcgaccg cttccgcgaa ggccgttaca gggccgtcat
     4501 agcctcagac gtcctgaacg aaggggtcga cgtacccgac gcgggggtcg ccgtcatcct
     4561 cgccggcagc gcctcccgcc gcgaatatgt ccagcgcctt ggccgcatcc tccgcccgag
     4621 ggaaggcaag cgggccgtcc tctacgagct cgtcacggcc cagacaggcg aagagttcac
     4681 ggcccgccgc cgcaaggagg ccttcctcta gtgctacgca gcgagcacgt catagcccgc
     4741 ctgaaccgcg gcaggctcgt cccccaccga ctctcgcccg acgatcagcg tgtgacagat
     4801 gtcgccggcg aactttgcga tctctacgcg gcccacttag ggctcccgcg gtccagcctg
     4861 gagcgggagc tgacggttcg ggaggaagat ctcggtcccg gcctcgatgc gcggaggggc
     4921 ttcaagatcg tgcgcgccct cgcaaagctc cttgaggagc gggcgaattg gaccgagcct
     4981 acagaagccg acccatacac gctgcgcacc cgcatcttcg agctcgccgc ggccctgcct
     5041 gaaccaccgg gcacggagcc tggattgctc gaagccccga cgcgcgagga cgtgctctct
     5101 cgggtagccg tagagacagg ggtcgatgag cctgccgccc tcatgtacgc ggacaggcag
     5161 ggggcccaac tcctcgacgc cttcgaccgt ccctcgcctg aggagctgat cagacgctac
     5221 aacgtcgccc aggtacaggg tgttctgtac tcagcaaggg atctcgtcgt cgaccttggc
     5281 gaaagggccg acgccaggct cgtattccac tatgtgaagc ttctgggttt gatctaccgt
     5341 ctggaacctc tctcccacgg gcaccgtctc cacctcgacg gcccgctctc catcttcgga
     5401 ggcacccgca agtacgggct gcgtctggcc aagttcctcc ccggtctgct gatgacttct
     5461 ccctggaacc tctcggcaaa cgtggagtgg aagggcagag atactgtcct cgagctggat
     5521 tcgaagaaga gcggactgga gagccactac ctgggaccga aggtcgaacg agaggccgac
     5581 gatacccgcg aggcattcat ccgggcctgg gaacgtacga aggacaccgg gggctgggag
     5641 ctcgaaaggg gcggagacgt gctgcctttc ccccagaaga aggccgcgct tgtccccgac
     5701 ttcaccctgc gccacgcaga cacgggagaa gtggtccacc tcgagatcct tggcttctgg
     5761 tccgagcgca acctcgtcga acgagtcgcc cttatcaggg aggccgggga gcagggccac
     5821 cgggtgctcc tcgccgcctc ggagaggctc ggcgcgtcac aggaggccct ctccggggcc
     5881 gtggaaggag gcgtcgtacc tttcaaggac cggctcgccg ccaaggccgt acttgccgcg
     5941 ctcgccgagc gcgacgctac gctagcgtag cgccctcgcc ctcgaaaccg cctcgtaggc
     6001 gtcgaggtag cccgccccca cctcgaactc tgcgtagccc ggcatgggcg tagccgtggc
     6061 tgtaatgaca cgttcgatcg cgtcaggtcc cagggccggg ttcgcctgct ccatcagcgc
     6121 gaccgtacct gacacgtgcg gggtcgccat gctcgtgccg ctcgccgtcg tatagtacgg
     6181 cagccattcc gttctcggta tgtacgttgc gtcgtcggcg gcggccagcc cgttgatggt
     6241 cgcgccagtc gaggcccgcg cggagacgat gttcacacca ggcgcggtga tgtccggatg
     6301 ataaaggttg tctcccagca cgcccctgga cgagaagctc gccagggtct tcccgtcctt
     6361 cttgccggcg gccacaccga tcacccacgg cgccacggag tacgggttga gagtatcttt
     6421 accgggtccc tcgttgccgg cggcgaatgt gaccgtcatg ccgcggtcgt gggccagctt
     6481 agaggcgcgg ttgatcgggt cgttcctgtc gaacttgccg ctcgtgcccc aggagttcga
     6541 gatcacgcgt atgtcgtact tttcctggtt ctggagcgcg tagtcgaatc cctcaagcgc
     6601 gtagaggatg agcagcgtgt cccctgttcc tatcccgacg aggtccgagc ccggcgcgac
     6661 gcccgtgtag tagccgccgc tgttggcccc gctgccgccg accgtcccgg ccacgtgcgt
     6721 accgtggccg ctggaggtgt cggtgttctt ctggttctcg aggacgaccg tgttcccggt
     6781 gaagaaagcg tcgccggcga tcttcacgtt ctgcacgagc cgtccggggt agcttacgtc
     6841 gctgttagtg ccgtccacgc ccgagtctat gacggccacc ccgacccctg tgccgtcgta
     6901 cccgagcccc gacctcaccc tgttggcccc gatggtcctg acgctctcgt gcagcaggta
     6961 atcgagcttg cggtccgcgt agacggagac cggtgcccac atgtcgagca aacgcccaac
     7021 ctggctcttc gtccccctga cgcccagcat cggcagcacg tcgaagcggt gcacatctac
     7081 gcctgtccgc ttcacgtttt gcacctgcgt cgcgctcggc ttttcatcgt aggtcagaat
     7141 cgcgcgcacc tgcgttccgg cttccgcgcc ggcgagcgtc tcctggagac ggtcgtccac
     7201 caccggccca gcgctctgag ctcccccggt gcccgcggcg aacagcgcca gggcgacgaa
     7261 cgccaggatc gcggaaatga ctagcgtatt gatgcttcgg gttggggcca aggctcctcc
     7321 ctcggacgcg aacagagtta taaatgttac tatgccggcg tgaggcccgc aaaactcgtc
     7381 acctgacgcg agagccatat aatccctacc atggaaagaa acacagggtc cctgatcggc
     7441 ctcaccgtcg cctgcaccat ctcagccgtc atcttcgggt tcggcgccga gatgttctcg
     7501 tggaggagcg cctacgaggg tctcggcaga gagtcactaa tacagacgac ccgccttgtc
     7561 gtctacgtga cgctcggcgt cctgctcgcg ttccgcggcg gctggcacgg ggtactcgcc
     7621 gccatcgcga tggctttcgt ggccacctcc gtggagtggg cgctctatcc cttctcctac
     7681 gcctgggcat ccatagagga tcccgcaggc tacgcggaca ccttcggcgg cgtgcagcgt
     7741 cccgactacg ccttctggac cacctacgac attatcgggg tggggatctc cgcggccctc
     7801 gcccagggac tgcgtacgat ggcccaggtc aaccccaggg atctctaggc cgcacgacct
     7861 caagctcctc atcctcgtcg aagtcgagga gatatacctc gccgagggca ttgaaatcgt
     7921 agcgcgaacg cggcaggact ccctccacgg agtcctcgat ctcgacgccg gtcacgagcc
     7981 tgccccttcc cctgcaccgc ttgcactccg tcgggcgcca gg
//
LOCUS       DATAIR010000035         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_278960, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000035 DATAIR010000000
VERSION     DATAIR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278960"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHM69_00980"
     CDS             <1..796
                     /locus_tag="VHM69_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2738990.1"
                     /translation="PTVVWTDYSHLYERAARRRLAAQVDKIEAAGGSVAGQHLLHGPA
                     IDALLDLCEELHPGLLVTGSRGLGAVRRVGLGSVSEGVVHHAHCPVLVVRGGGDAWPP
                     EKVVIGDDGSEGAWAVAKLAAGIATACEAGVALVRAYRNPPEPIGGWSAGDRRMLDRM
                     RSVEWEALGERAEMLRAVSESQPTTKLVEGDAAHRLLVAGGGDDGRNVLLAVGSRGIG
                     PLRRMRLGSVSTSVLRGAEGTVLVCPPSARDAVPAGHATASEGVVQ"
     gene            827..1354
                     /locus_tag="VHM69_00985"
                     /pseudo
     CDS             827..1354
                     /locus_tag="VHM69_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            1380..1868
                     /locus_tag="VHM69_00990"
     CDS             1380..1868
                     /locus_tag="VHM69_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738991.1"
                     /translation="MNYHAPQHIGTTHADEGYRTQTPHSTQTFAGVEAARADRAFEGR
                     QRYRLLRAKDTLLRELASETSPRRRATGERGGSGTPVCVTDSRTLAGELEAMLERKLR
                     LRLERVERALEKVAQGTYGTCDVTGERIPRERLERVPEEVETAEGRRRLLATGGRIRG
                     RS"
     gene            1888..>2087
                     /locus_tag="VHM69_00995"
     CDS             1888..>2087
                     /locus_tag="VHM69_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2738992.1"
                     /translation="MRAMILEGSGKPLRAVDVPIPEVGPGQVLLRVRACGVCRTDLHI
                     LDGELTDPKLPLIPGHQIVGVV"
BASE COUNT          368 a          620 c          802 g          297 t
ORIGIN      
        1 cccgaccgtg gtgtggaccg actactcgca cctgtacgag cgcgcggcgc ggaggcggct
       61 ggcggcgcag gtggacaaga tcgaggcggc gggtggcagc gtggcgggac agcatctgct
      121 gcacggtccc gccatagacg cgctgctcga cctgtgcgag gagctgcatc cagggcttct
      181 tgtaacgggc agtcgcggcc tcggcgcagt gcgccgcgtg ggcctaggga gcgtgtcgga
      241 gggcgtggtc caccacgccc actgcccggt cctcgtggtg cgcggcgggg gggatgcctg
      301 gccgccagaa aaggtcgtca tcggcgacga tggctccgag ggggcctggg cggtcgcgaa
      361 acttgcggcg ggcatcgcca ccgcctgcga agccggagtc gcattggtcc gggcgtaccg
      421 gaatccgccg gaacccatcg ggggctggag cgcgggggac cgtcgcatgc tcgaccgaat
      481 gaggtccgtg gaatgggaag ccctgggtga gcgggcagaa atgttgcggg ccgtctcgga
      541 aagccagccg acgacgaagt tggtagaggg ggatgccgcg catcgcttgc tcgtggctgg
      601 cgggggggac gacggacgga atgtccttct tgcggttggc agccggggga tcggaccttt
      661 gcgacgcatg aggctcggca gcgtctccac gagcgtgctc cggggcgccg aagggacggt
      721 gctggtctgc ccgccctccg cgagggatgc ggtaccggcg gggcatgcta ccgctagcga
      781 aggagtcgtg caatgatcga aggcgtcttg ccggccgctc cgccgcctcg tgctggggag
      841 cgtgtcggag ggcgtggcgg accacgccca ctgcccggtc ctcgtggtgc gcgacggggt
      901 ggatgcctgg ccgccaggaa gggtcgtcat cggcaacgag gggtcggatg cgtccatggc
      961 ggcgggcgag ctggccgctg gcatcggcag gcttttcggg gccggcatgc tggtagtgcg
     1021 cgcggcaccg gaccccacgc tgccgatgca gttgccggag tacgaaaaga atctgcacga
     1081 actgttggtc gagaagagca cacgcctggg cgagaaagcg ctcgaagaaa gggctggcgc
     1141 gctgcgcgag attctcggga cgcagccaga gacgaggttc gcgatgggag acgcgggcca
     1201 tcctcgaagc aaccgcggac gagccttcga cgcttatggc cgtgggctcc cggggaaccg
     1261 gtgcggcgaa gcggatcctg cttggcagcg tctcgaagaa actgctcagg atggcgaagg
     1321 ggcccgttct tatccatcct cggccaacgg ctgaacaaac gaggtagcga ggggtagatt
     1381 tgaattatca tgcaccacaa cacatcggga ccacgcacgc cgacgaaggc taccggacgc
     1441 aaacgccgca cagcacccag acttttgctg gcgtcgaggc cgcgcgagcc gaccgggcat
     1501 tcgagggccg gcagaggtac cggctcttgc gggccaaaga caccctcctg cgcgagctcg
     1561 ccagcgaaac gagccccagg cggcgggcga ccggggaaag aggtgggtca ggcacaccgg
     1621 tctgcgttac cgactcccgg acgctagcgg gagaactgga agccatgctc gaacgcaaac
     1681 tcaggctcag gctggagcgg gtcgagcgcg cgctcgaaaa ggtcgcgcag ggaacctacg
     1741 gcacctgcga cgtcacggga gagaggatac ccagggagcg tctggagcgg gtgccagaag
     1801 aggtcgagac cgcggaaggc cgaaggcgcc tgcttgcgac gggcggtagg atacgcggcc
     1861 gatcttgagc gtagaggggg agtggcgatg cgggccatga tcctggaagg atcaggcaaa
     1921 ccgctgcggg ccgtggacgt gccaatcccc gaggtgggac cggggcaggt tctcctgcgg
     1981 gtccgcgcct gcggggtgtg ccgcacggac cttcacatcc ttgacggcga gctaaccgat
     2041 ccgaagctac ccctgatccc aggccaccag atcgtcggtg tagtgga
//
LOCUS       DATAIR010000036         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_417282, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000036 DATAIR010000000
VERSION     DATAIR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417282"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VHM69_01000"
     CDS             complement(<1..527)
                     /locus_tag="VHM69_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2738993.1"
                     /translation="MATATKFDVGQMRVHILDDGVFVTDAGNLFGGSRKASIKGAMHP
                     VLVEAGDDVVLIDAGFGPELPEILVGRYELRRDENLLDHLDKSGYAAEAVTHVILSHL
                     DADHVGWALNPPSFPNATVYVQQAALEEARKMPEKDGRRAAVPAVEEGVKEGWCELLN
                     MDGDVLPGIRVEVRAG"
     gene            720..1769
                     /locus_tag="VHM69_01005"
     CDS             720..1769
                     /locus_tag="VHM69_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2738994.1"
                     /translation="MTRNWWVMLALVAFLIPLLLLGGNSASADPSYDSEEIEFLGLIN
                     EYRQQNGVGPLILSDTLTMSSEHHSQDMARYGFFAHNTVASSYYARGAQPWDRMVAEG
                     YAYNTHKGENIATGYDTAEEVFQGWRESPSHNAAMLDGNYRVIGISRLYAPSNSHSWY
                     WPTDFGGYVDPTSHGPGELPQAQEPTSQPVQDPNEEDKEQRTEDKGGIENSSMNTQAI
                     WRQKARDGADLILEEGYARLGGYDDGVDDLSQKISVGKETRLVYGLRIKTEEQTEERT
                     HPSDRLVVRVTNKDGEQLAVLRRYTDEDAGARRRPKVNLSRFAGRTVYLSFHVVTDEA
                     ELTSFYLDNVDLKRG"
     gene            complement(1779..>2307)
                     /locus_tag="VHM69_01010"
     CDS             complement(1779..>2307)
                     /locus_tag="VHM69_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2738995.1"
                     /translation="NLKDFYDRFLEEPLMADWLENATPEGPPTSWSLKMGMWGARRYG
                     QGMMLVGDAGSMIHPISGEGVGYALESGRLAASWANEAHVRQDFSASTLSGYERQLRH
                     RRAREHISGYALVNLVPNLETLEPLFKACEKDTEARRTLVESFTGDAPVYSLLKHPRA
                     LAKALRDGVTERIRS"
BASE COUNT          456 a          734 c          700 g          417 t
ORIGIN      
        1 ccagccctga cttctaccct gatacctggc aagacgtccc catccatgtt gagtagctcg
       61 caccagccct cctttacgcc ttcctcgacc gcagggaccg ccgcccgtcg tccgtccttc
      121 tccggcattt tccgagcctc ctccagagcg gcctgctgca cgtagacggt ggcgttgggg
      181 aagctcggcg ggttcagggc ccagccgacg tggtcggcgt caaggtgcga gaggatgaca
      241 tgcgtaaccg cctccgcggc gtaacccgat ttgtccagat ggtcgagaag gttctcgtct
      301 ctcctaagct cgtagcggcc gaccaggatc tcgggcagct cgggaccgaa tccggcgtcg
      361 attagcacga cgtcatcgcc ggcctcgacg aggacagggt gcatggcgcc ctttatgctg
      421 gccttgcgcg agccgccgaa caggttgcca gcgtctgtaa cgaacacccc gtcgtccaga
      481 atatggaccc gcatctgccc tacgtcgaac tttgtcgcgg tagccaagtc gccgaagcct
      541 ccttgatcgt gaacccgaat gatagcgggc ccgggggccc tacgcttgct gcagggagca
      601 aagtggggat cgtcagcccg ataggatcaa aatgatctag cccattttgg gtatggcgcc
      661 agtgcgcgcc cggctgcata attcctgcca tggcgtcggc tgggcagcat gaccgtaggg
      721 tgacccgcaa ctggtgggtc atgctagcgc tggtcgcatt cctgatcccg ttactcctct
      781 tgggagggaa ttccgcatcc gccgaccctt cctatgattc agaagagatc gagttcctcg
      841 gtctcataaa cgaatatcgt cagcagaacg gcgtggggcc tttgatcctc tccgacaccc
      901 tcacgatgtc ctccgagcac cattcacagg acatggccag gtacggtttc ttcgcccaca
      961 ataccgtagc gagctcatac tatgcgagag gcgcccagcc ctgggaccgc atggtggccg
     1021 aaggatacgc ctacaatacg cacaagggcg agaacatcgc caccggctac gacaccgccg
     1081 aagaagtctt ccagggctgg cgcgagtcgc cgtcccacaa cgcagccatg ctcgacggca
     1141 actaccgcgt gatcggcata tcccgcctct atgcgccttc aaacagccac agctggtact
     1201 ggcccacgga ctttggggga tacgtggatc caacatccca cggccccggg gagcttccgc
     1261 aggctcagga acccacgagc caaccggttc aggatccaaa cgaagaagat aaggagcagc
     1321 ggacggaaga caagggcggt atagagaact cttcgatgaa cacacaggcc atctggagac
     1381 agaaggccag ggacggggcg gatctgatcc tcgaagaagg ctacgcccgt ctgggcggct
     1441 acgacgacgg ggtcgacgac ctgagccaga agatcagtgt cggcaaggaa accaggctgg
     1501 tctacggact caggataaag acggaggagc agacggagga acgtacgcac ccgtctgacc
     1561 ggctcgtggt cagggtgacc aacaaggacg gcgaacaact cgccgtactg aggaggtaca
     1621 cggacgagga cgctggagcc aggagacgcc cgaaggttaa cctctcacgc tttgccggca
     1681 ggaccgtcta tctgagcttc cacgtcgtga ccgacgaggc cgagctaacc tcgttctacc
     1741 tggacaacgt cgacctcaag agaggttgac gtgggtcttc aggaccttat cctctccgtg
     1801 actccatccc tgagggcctt cgcgagcgcg cgggggtgtt tgaggaggct gtagacgggc
     1861 gcgtcccccg tgaagctctc caccagcgtt ctgcgtgcct cggtgtcctt ctcgcaggcc
     1921 ttgaagagcg gttcgagcgt ctcgaggttc ggcacgaggt tgaccagcgc gtacccggag
     1981 atgtgctcgc gagcccgcct gtgcctgagt tgcctttcgt agccggacag cgtggaggct
     2041 gagaagtcct gccttacgtg cgcctcgtta gcccacgagg cggccaggcg gcccgactcc
     2101 agggcgtacc cgacgccctc accgctaatg gggtggatca tgctccccgc gtcaccgaca
     2161 agcatcatcc cctggccgta gcgcctggcg ccccacatcc ccatcttgag cgaccagctc
     2221 gtcggcggac cttccggcgt ggcgttctcc agccagtctg ccatcagcgg ctcctcgagg
     2281 aagcggtcgt agaagtcttt gaggttg
//
LOCUS       DATAIR010000037         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_278964, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000037 DATAIR010000000
VERSION     DATAIR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278964"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VHM69_01015"
     CDS             <1..624
                     /locus_tag="VHM69_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2738996.1"
                     /translation="STGSPATAHPWTARRPALIEQVTLSMDGFEVNSYVVHAPEGDLI
                     VDAGAEPEKILDATRAPVMAILVTHGHADHVNALEFVRRETGVPVYMHPVDAEGAGVR
                     DYEPLEDGQSLAFAGLGIRVLHTPGHSPGSVTFVVGRDQIVGDLILPGSVGRTDISGA
                     SWEELEDSIRKVMPLWEEETRLYTGHGPVLTAAEELGTNPYLPPGLP"
     gene            621..1898
                     /gene="hisS"
                     /locus_tag="VHM69_01020"
     CDS             621..1898
                     /gene="hisS"
                     /locus_tag="VHM69_01020"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX2738997.1"
                     /translation="MTEYRGPRGTYDVFPGGREPHERPELWTFVEDRARDLFARYNYS
                     EIRTPIFEEARLFARTAGEGSDIVVQKEMFTFTDKGEREMALRPEGTPGAVRAYIEHN
                     MFKLAQPVKLFYIGPMFRQERQQKGRYRQHTQIGVEALGTSDPLVDVEVISLLYAIHS
                     AVGVKDEVVYMNNLGDMETRQAYVPELRAYLQRHSADLDPDSVARMETNPLRTFDSKH
                     DGTQAVLAEAPRITSFLSPEASAHLAAVREGLDELGIPYEIDERLVRGLDYYTMTVFE
                     AKSSALGAQDTVGAGGRYNALISDLGGPDLGGIGFGTGVERMLLAAASQETKTSLDVF
                     FVTLASEARIPAMRLAGALREEGISADLDYGERGMRAQFKQADRSGAAFAVILGEDEL
                     ARGVCTVRDMSSGEERVVPVAGGAKDLIRAVAG"
     gene            1908..2639
                     /locus_tag="VHM69_01025"
     CDS             1908..2639
                     /locus_tag="VHM69_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX2738998.1"
                     /translation="MPDFPPDLIVALRRAARIVVLTGSGVSAESGVPTFREAQTGLWA
                     RFDPQELATPEAFERDPGLVWDWYEWRRKLVMEASPNPGHLAIAELEHLVPEFSLITQ
                     NVDGLHQRAGSRRVIELHGNILRSKCSAEGITVEDYEEAEKPPCCQSCGAPLRPDVVW
                     FGEMLPFEAIEAASEAARGSDLFLSVGTSSLVYPAAALPYEALENGATLVEINPEETP
                     LTPHAGYSVRALAGEALPELVSVFS"
     gene            2857..3426
                     /locus_tag="VHM69_01030"
     CDS             2857..3426
                     /locus_tag="VHM69_01030"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX2738999.1"
                     /translation="MEAALLLAAGVGDLGALAGLPPELAVRRAVPILAGVTLDPAFLD
                     ANIFPYLDEARRAEDWYVARQFDAGDGSYSIQVFVWPPGSRTQIHDHTTWGAYHCVLG
                     SVTEERYERLDDGTRPDHARLKKVWRLSWSSQEGISTVLPYDEGIHSVGNGNSETAVS
                     VHLYGPKLTDMDGRDYDPSRNYVCDRFDA"
     gene            complement(3456..>3907)
                     /locus_tag="VHM69_01035"
     CDS             complement(3456..>3907)
                     /locus_tag="VHM69_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739000.1"
                     /translation="DADTIVAPDWLAAQLRAVSEGARAIGGRIELADDGSLPEGVARK
                     HAEHGRLRHGRLLSDPDGLGKMEHWQFSGASLALTAEVYKEVGGLQPRTALEGEHLER
                     VPRQHSIPIHRLLSVRATTSARRVGRAERGLSYDLSQIARSLHREEG"
BASE COUNT          707 a         1213 c         1295 g          692 t
ORIGIN      
        1 tctacagggt cgcccgcgac ggcacacccc tggaccgcta ggaggcccgc cctgatcgag
       61 caggtcacac tctccatgga cggcttcgag gtgaactcat atgtcgtcca cgccccggag
      121 ggggatctca tagtcgacgc cggggcggag ccggagaaga tcctggacgc cacacgcgct
      181 cctgtaatgg cgatcctggt tacgcacggc cacgccgatc acgtgaacgc cctggagttc
      241 gtgcggcggg agacaggagt cccggtctac atgcaccccg tcgacgcaga gggtgccggg
      301 gtcagggact acgagccact cgaagacgga cagtcgctcg ccttcgccgg gctcgggatc
      361 agggtgctcc acacgcctgg gcactcccct ggctcggtga cgttcgtggt tgggcgggat
      421 cagatcgtcg gtgacctgat ccttccaggg agcgtgggcc ggacggatat ttcgggcgct
      481 tcgtgggagg agttggaaga ctccattcgc aaggtgatgc cgctctggga ggaggagaca
      541 cgcctctaca ctggccacgg ccccgtgctc acggcggccg aggagcttgg gacgaaccct
      601 tacctgccgc cgggtctccc gtgacggaat acagaggacc gaggggcacc tacgacgtat
      661 tcccaggtgg gcgggagcca cacgagaggc ctgagctgtg gaccttcgtc gaggatagag
      721 cgcgcgatct tttcgcccgt tacaactact cggagatccg cacgcccatc ttcgaggagg
      781 ccaggctctt cgccaggacg gcgggtgagg gctccgatat cgtggtacag aaggagatgt
      841 tcaccttcac cgacaagggc gagcgggaga tggcgctcag gccggagggt accccgggag
      901 ctgtgcgcgc atatatcgag cacaacatgt tcaagctcgc ccagcccgtg aagcttttct
      961 acatcggccc gatgttccgg caggagcgcc agcagaaggg ccgctacagg cagcacaccc
     1021 agatcggggt tgaggccctc ggcacctcgg acccgctcgt tgacgtcgag gtgatctcac
     1081 tactctacgc catccacagt gccgttggcg tgaaggacga ggtcgtctac atgaacaacc
     1141 tgggcgacat ggagaccagg caggcctacg tacccgagct gcgcgcctac ctgcagaggc
     1201 atagcgccga cctcgacccc gactccgtcg cccgcatgga gacgaacccc ttgcgcacct
     1261 tcgactccaa gcacgatggc acccaggccg tcctggccga ggcaccgagg atcacctcct
     1321 tcctgagccc tgaagcctcg gcgcacctgg ccgcagtgcg agaagggctc gatgagctcg
     1381 gcatccctta cgagatcgac gaaaggctcg tgcgtggcct cgactactac acgatgaccg
     1441 tcttcgaggc caagagcagc gctctcgggg cgcaagacac cgtcggggcc gggggacgct
     1501 acaacgccct gatctccgac ctcggaggcc ccgatctcgg ggggatcggc ttcggcaccg
     1561 gcgtcgagcg gatgctcctg gcagccgcct cgcaggagac aaagaccagc ctcgacgttt
     1621 tcttcgttac gcttgccagc gaggcccgta tccccgcaat gaggcttgcc ggagccctgc
     1681 gcgaagaagg tatctccgcc gacctcgact acggagagcg aggcatgcgg gcgcagttca
     1741 agcaggctga ccgttcaggt gcggcattcg cggtcatcct cggcgaggat gagctcgcgc
     1801 gcggcgtctg cacggtgcgt gacatgtcgt cgggagagga gcgggtcgtg cccgtagcgg
     1861 gcggtgcgaa ggacctgatc cgggccgtcg caggctgagc cctggttgtg cccgacttcc
     1921 cgccagacct catcgtagcg ctgcgtaggg cggcgcgcat cgtcgtgctc acgggctcag
     1981 gcgtctccgc ggagagtgga gtcccgacct tccgcgaggc ccagacaggg ctgtgggcac
     2041 gcttcgatcc gcaagagctc gcgacaccag aggctttcga gcgagatccc ggactcgttt
     2101 gggactggta cgaatggcgg cgaaagctcg ttatggaggc aagtccgaat cctgggcacc
     2161 tggcgatcgc cgaactggag catctcgtcc ccgagttctc cctgataacc cagaacgtag
     2221 acggtctgca ccagagggca ggcagcagga gggtaataga gctgcatggc aacatcctgc
     2281 gctcgaagtg ctccgcggaa ggcatcacgg tcgaggacta cgaagaagcg gagaaaccgc
     2341 cctgttgtca atcgtgcggg gcaccgctgc ggcccgacgt ggtgtggttt ggcgagatgt
     2401 tgccatttga agcgatagag gcggcctcgg aagccgcccg cgggtccgac ttgtttctct
     2461 ccgtcggtac ttccagcctc gtctatcccg cggccgcttt gccttacgag gcgctggaga
     2521 acggagccac gctcgttgag ataaaccccg aagagacgcc gctcactccg cacgccggct
     2581 acagcgttcg cgctctcgcg ggcgaagcgt tgccggaact ggtatccgtg ttttcatagt
     2641 cgccctggac cgacttccat aagccgaagg cgcttcggag agccgcgaga tcgtcaggag
     2701 tctccatcct tcgctcgtgg caccagctgt attgatgtca cggcacctgg ccggaaggca
     2761 gcgggaaaga tgcagaaata cttcatttgg gggatgtgtc tggcatcgtc cggaaggata
     2821 cttatgtggc aagaaaaatt accaggatgg tgaaccatgg aggccgcgtt gttactcgct
     2881 gcgggagttg gcgatttggg agcgctcgcg gggcttccac cggagctggc cgtgaggaga
     2941 gcggttccca tcctggccgg cgtgacgttg gatcccgcct ttctggacgc gaatatcttt
     3001 ccctacctcg atgaagcgag aagggccgag gactggtacg ttgcccgcca atttgatgcc
     3061 ggtgacggct cctactccat acaggtcttc gtctggcctc ctgggagccg gacccagatc
     3121 cacgatcaca caacctgggg ggcttaccac tgcgttctcg ggtccgtgac cgaggagcgt
     3181 tacgagcgcc tcgacgatgg cacgaggccg gatcacgcgc gtttgaagaa agtctggagg
     3241 ctgtcgtgga gcagtcaaga ggggatctcg accgtcctgc cttacgatga aggcatccac
     3301 agcgtgggaa acggcaactc cgagacggcc gtctccgtgc acctctacgg tccgaagctg
     3361 accgacatgg acggacggga ctacgatcct tcgcgcaact acgtttgcga tcgcttcgac
     3421 gcctgagcga tcggttagga aagcgttctc tgtcttcagc cttcctctct gtgcaggctg
     3481 cgcgcgatct gggagaggtc gtaggagagg ccacgttcgg cacgtcccac gcgtcgggct
     3541 gaggtcgtgg ccctgacaga gagcaggcgg tgtatcggga tggagtgttg ccgcgggacc
     3601 cgttcgaggt gttcgccttc gagagctgtg cgcggttgca ggccgccaac ctctttgtag
     3661 acctcagccg tcagggcgag ggaggcacct gagaactgcc agtgctccat tttcccgaga
     3721 ccgtctgggt ccgagagaag ccgcccgtgg cgaagcctgc cgtgctccgc gtgcttgcgc
     3781 gcaacgccct ccggcaggga gccgtcgtcg gcgagctcga tgcgcccgcc tattgcccgc
     3841 gctccttcgg agacggccct gagctgggct gcgagccagt ccggcgctac gatcgtatcg
     3901 gcgtccg
//
LOCUS       DATAIR010000038         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94234, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000038 DATAIR010000000
VERSION     DATAIR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94234"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VHM69_01040"
     CDS             complement(<1..896)
                     /locus_tag="VHM69_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2739001.1"
                     /translation="MLLLLQEKTSELAGAADDVKKVTEDATREPLSIYRGIREFLDST
                     LGYVTSAGFIGDILASLAVVFLGLLIFRFMTRGIPRVLQWRRQREGLLNEEAVARIKR
                     QDTAITLTRNALRYVVFIIVTLVVVSIFIEDPLPGVAGATILAAVIGFGAQSFLRDVI
                     AGFSIVFEGQYSVGDFVLIKPQDVSGVVEELGLRMTKIRSLSGEVSYIPNGTMQGVVN
                     YVSGQQRFNVEVQVLDAEAASRVENALGETSELYMSPPRLVERDEKDGRVRMRIVAGV
                     LPSMAWLVEENLTAQIKAAAGE"
     gene            complement(964..1698)
                     /locus_tag="VHM69_01045"
     CDS             complement(964..1698)
                     /locus_tag="VHM69_01045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2739002.1"
                     /translation="MKNTGTRRYSDITVGDKNPAKRYLQRRRLRDALSVLDGLGDDFA
                     GSFLDFGGGSGELSKMLARRFPDAGVFCYEPMPGIFEEARRNLSDLENVTLVSDRRGL
                     RGLAFEYVFCLEVFEHLPRRQTERTLRTVNRLLKENGIFVVGVPNELFIAALVKGLFR
                     MKRRYGTYDARPKNVLRAAIGRPPARRPVRMISEALPYHFHHLGFDHRRLRTQLSESF
                     EQVREFGSPINSQLFSTEIYFVLVKQ"
     gene            complement(1759..2796)
                     /locus_tag="VHM69_01050"
     CDS             complement(1759..2796)
                     /locus_tag="VHM69_01050"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX2739003.1"
                     /translation="MMVVFGGRSGEHDVSLASARAMTEALSGSHEVVPVGITREGRWI
                     SSGEPMRELEAKSNYLPDGSQPISGPPASATEKLPAGLGSVDVVFPVLHGPYGEDGTI
                     QGMLELAGVPYVGSGVLGSAAGMDKPTMKKIFAHHGLPQVAWAALTRKQWEKNRETWI
                     QEIENALGYPCFVKPANLGSSVGIGKAENAKELSNAIDAAAQLDRRIIVEEGVDAREI
                     EVSVLGNDDPEVSVPGEIIIKKEGFYDYEAKYVEGGMELLAPAPIPDETAHEIRRIAA
                     TAYEAIDAAGMARVDFFLERGTERLLLNEINTIPGFTPTSVYAELWSASGLPYEELLD
                     RLIQLALERHG"
BASE COUNT          512 a          891 c          882 g          587 t
ORIGIN      
        1 tcctccccgg ccgcagcctt tatttgtgcc gtcaggttct cctcgacgag ccaggccatc
       61 gatggcagga ctcctgccac tatgcgcatc ctgacgcgcc cgtccttttc gtcccgctcg
      121 acgagacgcg gtgggctcat gtagagttcc gaagtctcgc cgagcgcgtt ctccacccgc
      181 gaggccgcct cggcgtccag gacctgcacc tcgacgttga accgttgctg gcccgagacg
      241 tagttgacga cgccctgcat cgtaccgttg gggatgtagc tcacctcgcc cgagagggac
      301 cggatcttgg tcatcctcaa ccccagctcc tcgacgacgc ccgagacgtc ctgcggcttg
      361 atgaggacga agtccccgac gctgtactgc ccctcgaaga cgatggagaa cccggcgatc
      421 acgtcgcgca agaagctctg cgccccgaat ccgataacgg cggcgaggat cgtggctcct
      481 gcgacacccg ggagcgggtc ctcgataaag atgctcacca ctaccagcgt gacgattatg
      541 aagactacgt acctcagtgc gtttcgggtc agggtgatgg ctgtgtcctg gcgcttgatg
      601 cgggccacgg cctcctcgtt gagtaaccct tccctctggc gtcgccactg caggactctg
      661 ggtatacctc gggtcatgaa gcggaagatg aggaggccga ggaataccac ggccaacgac
      721 gcaaggatat cgcctatgaa acccgcgctg gttacgtagc caagggtgct gtccaggaac
      781 tccctgatcc ccctgtagat tgacaggggc tctctcgtcg cgtcttcggt gactttcttg
      841 acatcgtcgg cggctccggc gagttcgctc gttttttcct gaagtaatag gagcatgtgg
      901 ctattaaagt attctccgcc tccgccttca aggtggatga ggccaggtct gagaggtctg
      961 tgtttactgc ttcacaagca cgaagtagat ctcggtgctg aacaactggc tgttgatggg
     1021 gctgccgaac tcgcgtacct gctcgaagga ctcggagagc tgggtacgca accggcgatg
     1081 gtcgaagcca aggtggtgga aatgataggg cagagcctcg gagatcatcc tcacgggccg
     1141 cctcgcgggt ggacgaccga tggccgcgcg caggacgttc ttcggtctgg catcgtatgt
     1201 gccgtagcga cgcttcatcc tgaacagtcc cttgaccagg gcggcgatga acagctcgtt
     1261 cgggacgccg acgacgaaga tgccgttctc cttgagcaga cggttgacgg tcctcaaggt
     1321 cctctccgtc tgacggcgcg gaagatgctc gaagacttcg aggcagaaaa cgtactcgaa
     1381 ggcgagcccc cgcagccctc tgcggtcgga gacgagagta acgttttcga ggtcggagag
     1441 gttccgacgg gcctcttcga agatgcctgg catcggttcg tagcagaaca cgccggcgtc
     1501 aggaaagcgc ctcgcgagca tcttgctcaa ctcaccgctg cccccgccga aatcgaggaa
     1561 gctgcctgca aaatcatcgc cgaggccgtc gaggacggag agcgcgtctc gcagtcgccg
     1621 acgttgcagg tagcgttttg ccgggttctt gtcgccgacg gtgatgtccg agtaacgccg
     1681 cgtaccagta tttttcatcc gagcatcccc tttacctgac ggtagatact cccgggagtg
     1741 gcagaggcag gcattctgct agccgtggcg ttcgagggcc agctgtatga ggcggtcgag
     1801 gagctcttcg tagggaaggc cgcttgcgga ccagagttca gcgtatacgc tcgtcggggt
     1861 gaagccgggg atcgtgttga tctcgttgag gagcaaccgc tccgtgccgc gttcgaggaa
     1921 gaagtcgacg cgtgccattc cagcagcgtc tatggcttcg taggccgtgg cggctatgcg
     1981 gcgtatctcg tgtgcggtct cgtccggtat gggggcaggc gccaggagtt ccataccgcc
     2041 ctcgacatac ttcgcctcgt agtcatagaa accttctttc ttgatgatga tctcacccgg
     2101 cacggagacc tccgggtcgt cgttgccgag gacggagacc tcgatctcgc gggcgtcaac
     2161 gccttcctcg acgattatgc gacggtcgag ctgcgccgcc gcgtcgatgg cgttgctcaa
     2221 ctctttcgcg ttttcggcct ttcctatacc gacgctcgac ccgaggttcg cgggctttac
     2281 gaagcaaggg tatccgagtg cgttttcgat ctcctgtatc cacgtctccc tgtttttctc
     2341 ccactgcttg cgggtcaggg cggcccaggc tacctgcggg aggccgtggt gggcgaagat
     2401 cttcttcatg gtcggcttgt ccatgcccgc cgcgctcccg agcacaccgc taccgacgta
     2461 ggggacgcct gcgagctcga gcatcccctg tatggtgccg tcctcaccgt acggcccgtg
     2521 cagaacgggg aagacaacgt ctacggaacc gagccccgcg ggaagcttct cggttgcgga
     2581 ggccggagga ccggagatag gttgggagcc gtcgggcagg tagttgctct tagcctcaag
     2641 ttcgcgcatt ggctcgcccg aggagatcca acgcccctcc ctggttatcc cgactggcac
     2701 gacctcgtga gagccactca gcgcctccgt catggcgcgc gctgaggcca gggatacgtc
     2761 gtgctccccg ctacgacccc cgaagacgac catcaccctg accaaagcgc cgctcctctc
     2821 ttctccaacc cttccatcct agcaaagggc gcgaaggtag ttctaagcct cc
//
LOCUS       DATAIR010000039         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94246, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000039 DATAIR010000000
VERSION     DATAIR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94246"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VHM69_01055"
     CDS             complement(<1..304)
                     /locus_tag="VHM69_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX2739004.1"
                     /translation="MDQRDQGTFRERIARNRRNSLLLIAAFLAFVTVFGYIIGFAWIG
                     DPVGALFGLVLALIVGIISGFVTYYAGDKMVLAASRAKEVTHDGAPVLFNVVEEMSI"
     gene            complement(308..868)
                     /locus_tag="VHM69_01060"
     CDS             complement(308..868)
                     /locus_tag="VHM69_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX2739005.1"
                     /translation="MGTILLILLVVIVVILVIYAIATYNGLVRRRNEVEESYRQIDVQ
                     LKRRYDLIPNLLEGVKKYFRQEQDVLTQITQARSQAMQPQSPGQQAQSEARLSGAIGN
                     LFAVAENYPELRSNTVLLDFQEELSSTENKISFSRQHYNDSVQSYNTKIQSFPAVIFA
                     RAMGFTEREYFEAQGPERESVTVSYD"
     gene            1070..1810
                     /locus_tag="VHM69_01065"
     CDS             1070..1810
                     /locus_tag="VHM69_01065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2739006.1"
                     /translation="MTEYDPLSDLYDLEYTHDYDLPFWLSLAERESGPLIEWGAGTGR
                     LAVPLAAAGNDVTAVELSGRMVERGKEKGGAVEWIVGDMRTAKPKRLYGLAVCAFNSF
                     LCLSSVDDALAFLRNASEHLAPRGLLGIEVSAFSPDELVDPPGGPDLRHDFTRDLPDG
                     QFDRFSVSRYDAATQLMHMRLFYEQYGVSGALESRRAHDLTIRIVGRSELNLMLQVAG
                     FEVEAVYGSFEGEPFTSTSDHLIVLARK"
     gene            complement(2072..>2320)
                     /locus_tag="VHM69_01070"
     CDS             complement(2072..>2320)
                     /locus_tag="VHM69_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739007.1"
                     /translation="AGRPRGGSRRKVFTVIVVPGTAALAGSTLIAISTTMVAASAAAN
                     KQSAANMLLVRYSDAFMIRCSLPLYGRGGGVARHTRLL"
BASE COUNT          474 a          692 c          690 g          464 t
ORIGIN      
        1 ctatggacat ctcctcgacc acgttgaaga ggacgggggc tccgtcgtgt gtgacttctt
       61 tggcgcggga cgcggcgagg accatcttgt cgccggcgta gtaagtgaca aagccggata
      121 tgattcccac gatgagtgcc agaacgagtc cgaagagagc acctacgggg tccccgatcc
      181 aggcgaaccc gattatgtag ccgaagacgg tgacgaacgc gagaaaggca gctatcagca
      241 gaaggctgtt acgccggttt cgggcgatgc gctccctgaa ggtgccctgg tccctctgat
      301 ccatcgtcta gtcgtaggag accgttacgg actcccgctc gggcccctgt gcctcgaagt
      361 attcccgctc cgtaaagccc atggcgcggg cgaagattac cgcagggaag ctctgtatct
      421 tcgtgttgta ggactggaca gagtcgttgt agtgctggcg ggagaaggag atcttgttct
      481 ccgtcgatga gagctcctcc tggaagtcca aaagtaccgt gttggacctg agctcggggt
      541 agttctcggc cacggcgaac aggttcccta tggccccgct gagtctagcc tcggactggg
      601 cttgctggcc cggtgactgg ggctgcatgg cctgggagcg ggcctgcgtt atctgcgtca
      661 gcacgtcctg ctcctggcgg aagtacttct tcacgccttc gaggaggttc gggatcaggt
      721 cgtaccggcg cttgagttgc acgtctatct gcctgtaaga ctcctcgacc tcgttgcgcc
      781 gccgcacgag cccgttgtag gtggcgatgg cgtagatcac cagtatcacc acgatcacga
      841 ccagtagtat cagcaagatt gtgcccatcc aatacctcca cgaataggga tgctaaagga
      901 attatatccc ccacggccaa ctaaggctcg atggactgga tcctgttccc aaaatgccct
      961 cgccacagga ccgtcgacac taactcacgt agcccagacc aaccaacatc ctacacgttg
     1021 atcttcgtgt agcctgaccg gagcagacgg cctacgagga aggagcaccg tgacggaata
     1081 tgatccctta tccgacctct acgacctcga atacacccac gactacgacc tccccttctg
     1141 gctctctctc gccgagcgcg agtccggacc cctcatagag tggggcgcag gcacgggccg
     1201 cctcgccgtc ccgctcgccg cggcaggcaa cgacgtcacc gcagtggaac tctccgggag
     1261 aatggtcgaa cgagggaaag aaaagggcgg cgccgtcgag tggatcgtcg gtgacatgag
     1321 aactgcgaaa ccgaaacgcc tctacggact cgccgtctgc gccttcaact cgttcttgtg
     1381 cctgagcagc gtggacgacg ccctggcgtt cttgcgtaac gcgagcgaac atctggcgcc
     1441 acgcggcctg ctcggcatag aggtctctgc gttctcgccg gatgagctgg tggaccctcc
     1501 gggcggcccg gatctgcgcc acgacttcac ccgagatctg cctgacggcc agttcgaccg
     1561 cttctccgtc tcccgctacg acgcggccac acagctcatg cacatgcggc tgttctacga
     1621 acaatacggc gtttcgggcg cactggaaag ccggagggcc cacgacctca ccatccgcat
     1681 cgtcggacgt agcgagctga atctcatgct gcaagtagca ggcttcgagg tagaggcggt
     1741 ctatggcagt ttcgagggag aaccgttcac ctcgacgagt gaccacctca tcgtgcttgc
     1801 acggaaatag gtttcaggct gcaggtttct gccacagatc gagggacgtg ggtggagttt
     1861 gtgtttcgag tcggtaggcc tttgtacaag gttgctcgaa gcgaggatgc ggatagcgca
     1921 ccgtcgcagg gaatctgatg aagggttatc tgctaaccgg gtggacacgg agttctcgga
     1981 aggttgccac atgctaataa ccatcagcaa cgccaaatat acgctacata gcgtatatac
     2041 atcccgctcc tcagtctcga tacttttgct actacagcaa gcgggtgtgg cgagccactc
     2101 ctccgccgcg cccatagaga ggcaaggagc agcggatcat gaaagcatcc gagtatcgca
     2161 caagaagcat attggccgcg ctttgcttgt tggcggcggc actggcggcg accatcgtgg
     2221 tgcttatggc gatgagtgtc gatcccgcta aggcagctgt ccccggcacc acaattaccg
     2281 tgaacacctt acggcgggat cctccgcgag gtctacctgc
//
LOCUS       DATAIR010000040         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_2114, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000040 DATAIR010000000
VERSION     DATAIR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VHM69_01075"
     CDS             <1..1302
                     /locus_tag="VHM69_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2739008.1"
                     /translation="LLQRQRDVLPDWIALYYEKPMELVRGEGFKVWDSEGNEYLDFFG
                     GIVTTISGHAIPEIVEAVREQAGKIFHSSTLYMIESQVKLAEKLISLSPIGGDQKVFF
                     VGSGSEANEAALLFATQYRNSSEIIALRGSYHGGSFGTMGITGQSAWRPTPRSALDVS
                     YAMTPHRSYSPLYGPFEGDPEGFARACAEDVQRLIETATTGRVATFIAEPIQGVGGFI
                     EIPPVYFQRVKEILDEHGVLFISDEVQTAFGRTGGHFWGIEDSGVEPDMITMAKGLGN
                     GLAIGAVMGRAELIDSLSPKLHISTFGGNPISAAGALANLEYVLKNDLQQNADEVGGY
                     LKERLRKMAEGNPFVGEVRGRGLMLAVEIIGPDGEPDPQGAIKFLQACRERGVLVGKG
                     GIKANAIRISPPLTITREAAEEAANAFEEALAEIGASARVG"
     gene            1302..>2167
                     /locus_tag="VHM69_01080"
     CDS             1302..>2167
                     /locus_tag="VHM69_01080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX2739009.1"
                     /translation="MQTEGVDARRAVEELKELAELTGGPDGARRVAWTDEWEKAREWF
                     RGKLEEIDGITDIETDEAGNVWATAPGDSEKAVLIGGHIDSVPNGGWLDGALNLVGAL
                     EVMRVLAPQQRPVTLRLVDWADEEGARFGRSLFGSGAAAGALKPDDVRNLKDRDGIAL
                     PDALKEHGVDLDRVNEAGKQLENAVAYLELHIEQGPVLERMDLPLGVVLGTFGVERHA
                     VRFTGQHAHSGSTPMEVRRDAFIAAARSAVDFRDDAKRRDDVRATTGFVNVSPAIVTA
                     FNGWCEMSLDQRA"
BASE COUNT          410 a          708 c          717 g          332 t
ORIGIN      
        1 cttttgcagc ggcagaggga cgtgttgcct gactggatcg cgctctacta cgagaagcct
       61 atggaactgg tgcgcggcga gggcttcaag gtctgggact ccgaggggaa cgagtacctc
      121 gacttcttcg gcggcatcgt cacgaccatc agcgggcacg cgatccccga gatcgtcgag
      181 gctgtgcggg agcaggccgg caagatcttc cactcctcga cgctttacat gatagagagc
      241 caggtgaagc tcgccgagaa actcatctca ctctctccca taggcggcga ccagaaggtc
      301 ttcttcgtcg gcagcggcag cgaggcgaac gaggccgcgc tgctcttcgc cacgcagtac
      361 cgtaactcca gcgagatcat cgcgctgcgc ggctcctacc acggcggctc gttcgggacg
      421 atggggataa cgggccagag cgcgtggcgt ccgacgccgc gctcggccct cgacgtcagc
      481 tacgcgatga ccccgcaccg ctcctacagc ccgctctacg gtcctttcga gggcgatccc
      541 gagggcttcg cccgggcgtg cgcagaagac gtgcagcgcc tgatcgagac agccacaacg
      601 gggcgcgtcg ccactttcat cgccgagcct attcagggtg tcggagggtt cattgagata
      661 ccgccggtct acttccagcg ggtaaaggag atcctggacg agcacggcgt actgttcatc
      721 tccgatgagg tccagacggc gttcgggcgc acgggcggcc acttctgggg catcgaagac
      781 tcaggcgtcg agcctgacat gatcaccatg gccaagggac tcggaaacgg acttgccatc
      841 ggcgcggtca tgggacgggc cgagctaata gactcgctat ccccgaagct ccacatctcg
      901 acgttcggag gcaacccgat ctccgcagcg ggcgccctcg ccaaccttga gtacgtcctg
      961 aagaacgacc tgcagcaaaa cgcagacgag gtcggcggct acctgaagga gcgcctgagg
     1021 aagatggccg aaggcaaccc gttcgtcggc gaggtccgcg ggcgcggact tatgctagcc
     1081 gtcgagatca tcggccccga cggagagcca gatccacagg gagctatcaa gttcttgcag
     1141 gcgtgccgcg agcgcggtgt gctcgtgggt aagggcggca taaaggctaa cgccatccgc
     1201 atctcgcccc cgctcaccat aacccgcgag gccgccgagg aggccgcgaa cgccttcgaa
     1261 gaggccctcg ccgagatcgg cgcttcagcg agggtcggct agtgcagacc gaaggtgtgg
     1321 atgcccgccg cgccgtcgag gaactcaagg agcttgccga actcaccggc ggcccggacg
     1381 gtgcccgccg cgtcgcttgg accgacgaat gggagaaagc ccgcgagtgg ttcaggggca
     1441 agctcgagga gattgacggc atcaccgaca tcgagaccga cgaggccggc aacgtctggg
     1501 ccacggcccc gggcgactcc gagaaggccg tccttatcgg aggccacata gactccgtcc
     1561 ccaacggcgg ctggctcgac ggcgccctga acctggtcgg cgccctcgaa gtcatgcgcg
     1621 tcctcgcccc gcagcagcga ccggtcaccc ttcgtctggt cgattgggcc gacgaagaag
     1681 gagcccgttt cggccgcagc ctcttcgggt ctggtgcggc cgcgggcgcg ctcaagcctg
     1741 acgacgtacg gaacctcaaa gacagggacg gcatcgccct gcccgacgcc ctgaaagagc
     1801 acggcgttga tctcgacagg gtgaacgagg cgggaaaaca actggagaac gccgtcgcct
     1861 acctggagct gcacatcgag caggggcctg tcctggagag gatggacctc ccgctcgggg
     1921 tggtgctcgg caccttcggc gtcgagcgtc acgcggtccg cttcacggga cagcacgccc
     1981 actccggctc cacgccgatg gaagtccgca gggacgcctt catagccgcg gcacgctcgg
     2041 cggtagactt cagggacgat gcgaagcgcc gcgacgacgt gcgtgctacg accggcttcg
     2101 tcaacgtcag cccagccatc gtaacggcgt tcaacgggtg gtgcgagatg tccctcgacc
     2161 agcgcgc
//
LOCUS       DATAIR010000041         8026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_371367, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000041 DATAIR010000000
VERSION     DATAIR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371367"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="uvrC"
                     /locus_tag="VHM69_01085"
     CDS             complement(<1..990)
                     /gene="uvrC"
                     /locus_tag="VHM69_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX2739010.1"
                     /translation="MTENGYPDRSHLPTSPGVYIFKDASDRVIYVGKAKNVRSRVASY
                     FTRSGDGRPKIAELRGRVRRIDFIVTGSETEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVVTTGDEYPRVFATRAAHDPRHRYFGPFPSAGSVHATLDVLNKTFPFRKCRGPEPG
                     RRSGVPCLNYHIGRSVAPCIGAVTKQEYDGIVADVIAFLEGRVDGLIRDRQEAMRVAA
                     SEMDFERAAKLRDEVSALSHVRDRQQATISSEDSFDAIGAFASGESACVQVFAVRGGQ
                     VVNRDSFLLDNYGESRAEDIALQFVPQYYDTAAVPREVLIQSDDREDELATLAD"
     gene            complement(1029..1766)
                     /locus_tag="VHM69_01090"
     CDS             complement(1029..1766)
                     /locus_tag="VHM69_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739011.1"
                     /translation="MSSNRDDPSQAEVTGLQPRYRRAFGCVTWLYMGMLTFLAANFLL
                     VLPSRYVGGGDLYGYLPPMLAFLGTALILPGMALAAVLGARSYRTTRRRGTRAGAGVG
                     ALIGWSGFFGLEWLAITFGLNRRDQAFRTVVFPDLDGSLAYYAFVPLTLVATALVIFA
                     LYSRRIDFERRRSLVIIGAALAVFAGLAIVATDFDPLGVMGALISTLAGAAGGYVSGA
                     GYARAGGDEMIPPGAEIPRREPRGKTR"
     gene            complement(1756..3255)
                     /locus_tag="VHM69_01095"
     CDS             complement(1756..3255)
                     /locus_tag="VHM69_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2739012.1"
                     /translation="MTSQDNNIQPKPVLLLVLDGIRPDVLQAAIREGDAPNLAAIAQR
                     GEAVWNAVSVFPSITPAATAAIATGEAPARSGIVGHAWYDEDERRVVIYGAMTETVMA
                     SGPIKVFHNNVWRMNRDDLHAATLFETLHDRGFDGACVNFPVRRGPHTHPIRMKTIEG
                     YLMGGRYLGPSVEGPKEYYMGDLFYSRETGFSGRRANGGVLRSVGINDDYASRVGAML
                     IKEKAAPFNLVYFFKGDSIAHHHGLEAQRRWLNTADGYVERIFRAGGGVERVLDDHAV
                     LALSDHGHAPLLPKHRYVDLRTIPGQKVSSGVKARFGNGTSVVVVPNGRSALLYLRND
                     VDLGPVVENLVGKRGVDLAVWTEGDWVAVRRLGREIRFRPGPGPRDPFGRSWELRGDP
                     EALDLRISENGDLRYGEYPDAMERLRGCLHSPRCGDVVLSAAPGYTFGEVSGGYHTAS
                     DHGSLHESDSNVFMLASGVPAPHRITDVAPTLLSHFGTRSPIVGEHSVV"
     gene            complement(3374..4831)
                     /locus_tag="VHM69_01100"
     CDS             complement(3374..4831)
                     /locus_tag="VHM69_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2739013.1"
                     /translation="MGIMRTKSIEQSIRDTEDPEFKLKKALGPLDLMLFGIGVIIGTG
                     LFVLTGEAAAGYAGPAVALSYVVGGIVCALAALCYAEFASTVPVAGSAYTFSYASLGE
                     FVAWLIGWDLILEFTLGAATVAKGWSGYLVSVLEILGITLPPSLYAATAGFSHDFVAV
                     FVVALVTTILVIGIKLSSQFNGVITSIKILVTIFIIGYGIFFIKVSNLTPFIPPPKPG
                     GEALQAYVLPSILGIDTIYGVTGIFTAAALVFFAYIGFDIVATTSEEARNPQRDLPIG
                     ILGSLAICTVFYVLASIVFTGLRPYQDLATAAPAATALQATPFPAAELIVSLAILIGL
                     TVVVMILILGQSRVAFAMSRDNLLPLWFSRVHTRFRTPHRITIITGTVAALLAFWLPL
                     TTLAELVNIGTLAAFVLVSIGVIVLRRSRPDLPRAFKVPGYPVVPILAAVSCFGLMGF
                     LTAGTWVRFFVWMAIGIVFYFAYSRSHSRLAREES"
     gene            complement(4933..5757)
                     /locus_tag="VHM69_01105"
     CDS             complement(4933..5757)
                     /locus_tag="VHM69_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739014.1"
                     /translation="MTAKSANPVVSPHHVMAAGLASAGAALFTSRFGVAGTLLGAALT
                     AMFITGATSILRAYLEAATGRVRQMPNKLRSSRRGQEKAGGFGGESTVPGHPELRNNF
                     MGRMRAAFGWFSNLPTLRRRSILMKGLVAAAIAFVICMGAVWGAEKLIGNSLSCGLWS
                     KCPYGAVPGIHPAGYGGTGAGTSIGGGSVDTGVEQPSLVDRVRQNPFGGDDQPVVGNP
                     GGAASSASPDPAASPDPAASPDPAASPDPAASPDPAASPAAKQQAAPSSASPAAGQ"
     gene            complement(5910..>8026)
                     /gene="uvrA"
                     /locus_tag="VHM69_01110"
     CDS             complement(5910..>8026)
                     /gene="uvrA"
                     /locus_tag="VHM69_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2739015.1"
                     /translation="GESMLFSESFTCTNCGASIAEIQPRTFSFNSPHGACDRCDGLGS
                     RLEIDPALVVPNEDLSVNEGAVVPWANTQTEYHRSVLGALSEKFGIDLDVPWRDLPEE
                     HRHLILFGTDGERIYVSYRNRYNRRRQYMTRFDGVVSNLERRYAETDSEYRREKIEEF
                     MSHVPCPKCRGARLRPEALAVTVGEKNIHEFAELSVTEAQRFFDGVEFTTREWIIGER
                     VVKEIRERLGFLVDVGLGYLTLSRSAGTLSGGEAQRIRLASQVGSGLVGVLYVLDEPS
                     IGLHQRDNRRLLTTLEKLRDLGNTLIVVEHDEETIRSADYVVDVGPGAGVHGGEIIAT
                     GSVEDVEAEERSVTGDFLSGRRRIEPPEERREPTGTIALLGAMEHNLKSVDVEFPLGV
                     FTCVTGVSGSGKSTLVNEILYKALANAVSRGKHRPGRHAGLRGVEDVDKVIDIDQSPI
                     GRTPRSNPATYTKVFDHIRQLFASTPEAKIRGYKPGRFSFNVKGGRCEACRGDGQIRI
                     EMHFLPDVYVPCEVCKGRRYNTETLRATYKGKSISDVLDMTVEEACGFFAPVPAISRR
                     METLRDVGLGYVRLGQPATTLSGGEAQRVKLASELGKRATGRTVYILDEPTTGLHFAD
                     VEKLLSILHRLVDAGNTVIVIEHNLDVIRSADHVVDLGPEGGDAGGEVVATGTPEDVA
                     RVEESHTGRFLRGLMPEVVAAS"
BASE COUNT         1457 a         2576 c         2569 g         1424 t
ORIGIN      
        1 atcggctagc gtcgccagct cgtcttccct gtcgtcggac tgtatgagga cttccctggg
       61 gacggcggcc gtgtcgtagt actgggggac gaactgaagc gcaatatcct cggcacgcga
      121 ctcgccgtag ttgtccagga ggaaagaatc gcggttcacg acctggccgc cgcgcacggc
      181 gaagacctgc acgcaggccg attcgccgct ggcgaaggct ccgatcgcat cgaaagagtc
      241 ctcagaggag atggtcgcct gctggcggtc gcggacatga gagagggcgg agacctcgtc
      301 gcgcagcttc gctgcccgct caaagtccat ctcgctggcg gccacgcgca tcgcctcctg
      361 gcggtcccgg atcagaccgt ctacccgccc ctccaaaaat gcgatgacgt cggccacgat
      421 cccgtcgtac tcctgcttgg tgaccgcccc gatgcaaggc gccaccgagc gcccgatgtg
      481 gtagttgagg cacggcacac cgctcctgcg ccccggctcg ggaccgcggc acttgcgaaa
      541 agggaacgtc ttgttcagca cgtctagcgt cgcgtggacg ctgccagcgc tcgggaaagg
      601 cccgaagtag cgatggcgcg ggtcgtgggc agcgcgcgtg gcgaagacgc gcgggtactc
      661 gtcgccggtc gtgaccacga tgtatggata actcttgtcg tccttcagcg aggcgttgaa
      721 gcggggcctg tggcgtttga tcaggttcgc ctcgaggacg agcgcctcgg tctcactgcc
      781 cgtgacgatg aagtctatgc gcctgacgcg ccctctgagc tccgctatct tcgggcggcc
      841 gtcgcccgag cgggtgaagt agctcgccac cctgctgcgc acgttcttcg ccttgccaac
      901 gtatatgacc ctgtctgagg cgtccttgaa gatatagacg ccaggggagg tgggaaggtg
      961 ggatctatcc ggatatccgt tctcagtcat cgcactggat tataccgcta agggttgcgt
     1021 ctccagggtc aacgagtctt gccgcggggc tcgcggcgtg gtatctcggc tcctggtggg
     1081 atcatttcgt caccacctgc cctggcgtaa ccggccccgc tcacgtagcc gccagcggca
     1141 ccggccagag tcgagatcag ggctcccatg acgccgagcg ggtcgaagtc cgtcgccacg
     1201 atagcgagcc cggcgaacac ggcgagcgcc gcgccgatga taacgaggct tcgcctccgc
     1261 tcgaagtcaa tccttcttga atagagggcg aatataacga gggcggtcgc gacgagcgta
     1321 agggggacga aggcgtagta agcgagactc ccgtcgagat ccgggaagac gaccgtacgg
     1381 aatgcctgat ctcgcctgtt cagaccgaag gttatggcga gccactccag gccgaagaag
     1441 ccgctccagc cgatcagggc tcccactcct gccccggcgc gcgtcccccg cctgcgggtc
     1501 gtcctgtagg aacgtgcccc gagcaccgcg gcgagcgcca tgcctggcag gatcagggcg
     1561 gtcccgagga aggccagcat gggcggaagg tagccgtaga gatccccacc gccaacgtac
     1621 ctggaaggca gcacgagtag aaaattggcc gcgaggaagg tgagcatccc catgtacaac
     1681 caggtaacgc atccgaaagc ccgccggtaa cgtggctgga gcccggttac ctccgcctgc
     1741 gaagggtcgt cgcgattaga cgacactgtg ctcgccgact atggggctcc ttgtgccgaa
     1801 gtgggaaagg agcgtcgggg cgacgtcggt gatcctgtgc ggagcgggca caccgctagc
     1861 gagcatgaac acgttggagt cgctctcgtg cagcgagccg tgatcgctcg ccgtatggta
     1921 cccgcctgag acttcgccga aggtatatcc tggcgccgcc gagagcacca cgtccccgca
     1981 cctcggcgaa tgcaggcacc cccgcagccg ttccatcgcg tcagggtact cgccgtagcg
     2041 cagatcgccg ttctccgaga tcctgaggtc cagtgcctcc gggtctcccc gcaactccca
     2101 tgatcgcccg aacgggtcgc gcggtccggg gcccggccgg aatcgtatct cacgccccag
     2161 ccggcggacc gccacccaat ccccctccgt ccagaccgcg aggtcgaccc cgcgcttccc
     2221 gacaaggttc tcgacgaccg gtcccagatc cacatcattt cgcaggtaga ggagcgcaga
     2281 tcgtccgttc gggactacga cgacgctggt tccgttgccg aatcgagcct tcacccccga
     2341 tgagactttc tggcccggta tcgtacgcag gtcgacgtag cggtgcttcg ggaggagcgg
     2401 cgcgtggccg tgatccgaga gggcgaggac ggcgtggtca tcgagcacgc gttcgacccc
     2461 gccgcctgct cggaagatcc tttcaacgta cccgtcggcc gtattcagcc agcgacgctg
     2521 cgcctccaga ccgtggtggt gggcgatgga gtcgcccttg aagaagtaca ccaggttgaa
     2581 cggcgcggct ttctccttga tcagcatggc cccgaccctg gaggcgtagt cgtcgttgat
     2641 gccgacggag cggaggacgc cgccgttggc cctgcgcccg ctgaaacctg tctcgcggct
     2701 gtagaaaagg tcgcccatgt agtattcttt gggcccttcg acggagggac cgagatagcg
     2761 tcctcccatg agatagccct cgatggtctt catccgaata ggatgcgtat gcggcccgcg
     2821 cctgacgggg aagttgacgc aagcgccgtc gaagccgcgg tcgtgcaggg tctcgaagag
     2881 ggtcgcagcg tgcaggtcgt cgcggttcat gcgccatacg ttgttgtgga agacttttat
     2941 gggaccgctg gccatcacgg tctccgtcat ggcaccgtag atgaccacgc gccgctcgtc
     3001 ctcgtcgtac caggcatgcc cgacgatccc gctcctggcc ggcgcttctc ccgtggctat
     3061 ggcagccgtc gccgccgggg ttatgctcgg gaagaccgag accgcgttcc agaccgcctc
     3121 tcccctctgg gcgatagcgg cgaggttcgg cgcgtccccc tcgcggatgg ccgcctgcag
     3181 cacgtcgggc ctgatgccgt cgagcacaag gagcagaacc ggtttgggct ggatattgtt
     3241 atcctggctc gtcaagaccc gctaattcta tccctttggg cgccggtcgg agcgaacccc
     3301 tccatacgag agaaagagcg gaccggagcc acgcactgta gctccggtcc gccgtgagtt
     3361 caggtctcgc gcgtcaggac tcctcgcgcg ccaggcggct gtggctgcgg ctgtaggcga
     3421 agtagaaaac gattcctatc gccatccaga cgaagaacct gacccaggtg cctgcggtca
     3481 ggaagcccat gaggccgaag cacgagacgg cggcgaggat gggcactacg gggtagccag
     3541 gcaccttgaa cgcccgcggg aggtctggcc gcgaccgccg gagtacgatg acgccgatgg
     3601 agaccagcac gaaggcggcg agcgtcccga tgttgacaag ttcagcgagc gtcgtcagcg
     3661 gtagccagaa cgcgaggagc gccgcgacgg tgcccgtgat gatcgtgatc cggtgcggag
     3721 tccggaagcg ggtgtgtacc cgggagaacc acagcgggag aaggttatcc cggctcatag
     3781 cgaaggccac ccggctctgg ccgaggatca agatcatcac gaccaccgtg agcccgatga
     3841 gtatcgcgag tgagacgatc agttccgcag ccgggaaggg cgtcgcctgg agggccgtgg
     3901 ccgccggggc cgcggtggcc agatcctggt agggcctgag cccggtgaag actatggatg
     3961 ccagaacgta gaagaccgtg cagatggcca gggagccgag gatcccgatg ggcaggtccc
     4021 tctgggggtt gcgtgcctcc tcggaggtag tggccacgat gtcgaagccg atgtaggcga
     4081 agaagaccag cgccgccgcc gtgaagatgc cggtgacgcc gtagatggta tctatcccaa
     4141 ggatactcgg caggacgtac gcctgaagcg cctctccgcc cggcttcggc ggcgggatga
     4201 agggcgtaag gttcgacacc ttaataaaga atatcccgta gccaatgata aatatcgtca
     4261 cgagtatctt gatagaggtg atcacaccgt tgaactgtga gctcagtttg atacctatga
     4321 ccaggatggt agtcacgagc gcgaccacga acaccgccac gaagtcgtga gagaagccag
     4381 ccgtcgcggc atacagtgac ggcggcagcg tgatcccgag gatttcgagc acgctgacga
     4441 ggtatcccga ccatcctttc gccaccgtcg cggcgcccag agtgaactcc aggatgaggt
     4501 cccacccgat cagccaggct acgaactctc ccagcgaagc gtacgagaag gtataggcgc
     4561 tgcctgccac cggcacggtc gaggcgaact ccgcgtagca cagggcagcc agggcgcaga
     4621 ctatgccgcc gaccacgtac gaaagggcca ctgcgggtcc ggcgtaacct gctgccgcct
     4681 ctccggtgag gacgaaaagg ccagtcccaa tgatcacccc gatgccgaag agcattagat
     4741 ccaacggccc gagcgctttc ttgagtttga attcgggatc ctcggtgtcc cggatggact
     4801 gctcgatgga ctttgtccgc attatcccca taacactccc ctttcccaat tcagctcgca
     4861 agctgatact tggagagcat tataaatgtc aacgctacgc cgtgcttgtg agagggttaa
     4921 ggggagatgc cgctactggc cggcggcagg cgaagcgcta gacggcgcgg cctgctgttt
     4981 cgccgcgggt gatgctgcgg gatccggcga cgcggcagga tccggtgacg cagcagggtc
     5041 gggtgaagcg gcaggatccg gtgacgcggc ggggtcgggc gaggccgatg aggcggcgcc
     5101 tcccgggttc ccgacgacgg gctggtcgtc gcccccgaac gggttctgcc gaacgcggtc
     5161 gacgaggctt ggctgttcga cgcccgtatc gacgctgccg cctccgatcg aagtccctgc
     5221 gcccgtgccg ccgtagccgg cgggatggat gcctggtacg gcaccgtaag ggcatttgga
     5281 ccaaagcccg caggagaggc tgttgccgat aagtttctca gcgccccaca ccgcgcccat
     5341 gcagatcacg aacgcgatgg ccgcggccac cagccccttc ataaggatcg agcgccggcg
     5401 gagggtcggc aggttggaga accagccgaa cgccgcccgc atcctgccca tgaagttgtt
     5461 cctgagctcg gggtggccag gcaccgtgct ctcgccacca aaacctccgg ccttctcttg
     5521 cccgcggcgc gacgaacgca acttgttcgg catctggcgt acgcgccctg tggcagcctc
     5581 gagataagcc ctcaagatag acgttgctcc ggtgatgaac atggctgtca gggcggcccc
     5641 gagcaacgtg ccggcgaccc cgaatcggga ggtgaaaagc gccgccccgg cggaggcgag
     5701 gccagcggcc ataacgtggt gcgggcttac aacaggattt gcgctcttag cagtcacagg
     5761 tgttgagcct atcagccggt ccacatcgtt tcaaagggtg agactgctat tttgtgaaga
     5821 aattctcgtt gcccgcctat tggtcccgga gttcgcagcg cagttccgcg agcctggagg
     5881 atatattcgc aggcaacggt gcccgagcgc tagctcgcgg ccacgacctc gggcatcaag
     5941 ccacgcagga agcggcccgt gtgcgattct tcgacgcggg cgacgtcttc aggggtacct
     6001 gttgcgacga cctcgccgcc tgcgtctcca ccctcgggac cgaggtcgac gacgtggtcc
     6061 gcggaccgga tcacatcgag gttgtgctcg atgacgatga cggtgttccc cgcgtcaacg
     6121 agcctgtgca atatggagag cagcttctcg acgtcggcga agtgcaaccc cgtcgtcggc
     6181 tcgtccagaa tgtagacagt cctgcccgtc gcccgtttcc ccagctcgct cgccagcttg
     6241 acgcgttgcg cctcgccacc agacagcgtt gtagcgggct gcccgagcct gacgtagccg
     6301 agccccacgt ctcttagagt ctccatgcgg cgtgagatcg caggcactgg ggcgaagaac
     6361 ccacaggcct cctcgacggt catgtcaagt acgtcggaga tggacttacc cttgtaagtg
     6421 gcgcgcagcg tctcggtgtt gtagcggcgg cctttgcaga cctcgcaagg gacgtagacg
     6481 tcggggagga agtgcatctc tatacggatc tggccatccc cacggcacgc ctcgcaccgc
     6541 ccgcccttga cgttgaaaga gaaacgtccg ggtttgtatc ccctgatctt ggcctcagga
     6601 gtcgaggcga agagctgcct tatgtgatcg aagacctttg tgtaggtcgc agggttagat
     6661 ctcggcgtcc ttccaatagg gctctggtct atgtctatga ccttgtccac gtcctcgacg
     6721 ccccgcaatc cagcatgccg tcccggacgg tgcttgccac ggctgacggc attcgcaagg
     6781 gctttgtaca ggatctcgtt gaccagcgtg ctcttccccg accccgaaac gcccgtcaca
     6841 cacgtgaaca ccccgagggg gaactccacg tcgacgctct tcaagttgtg ctccatcgcc
     6901 cctagcaatg ctatcgtccc cgttggctct ctccgctcct caggcggctc tatgcggcgc
     6961 ctgccggaaa ggaagtcgcc cgtgacagag cgctcctcgg cctctacgtc ctcgacgctg
     7021 cccgtggcga tgatctcacc cccgtgcacc ccggccccgg gcccgacgtc aacgacgtag
     7081 tcggcggaac gtatggtctc ctcgtcatgc tcgacgacga tgagtgtatt cccaaggtcc
     7141 cgcaacttct ccagcgtcgt caaaagccgc ctgttgtccc gctgatgcag ccctatagag
     7201 ggctcgtcga gcacgtacag aacgccgacg agcccgctcc cgacctgtga agccagccta
     7261 atcctctgcg cttcgccacc ggagagcgtc cctgccgagc ggctcaaggt gaggtatccg
     7321 agccccacgt cgacgagaaa accgaggcgc tccctgatct ccttgaccac ccgctcgccg
     7381 atgatccact cccgcgtggt gaactcgacg ccgtcgaaga agcgctgcgc ctctgtaacg
     7441 gagagctcgg cgaactcgtg gatattcttc tcccccaccg ttacggcgag ggcttctggc
     7501 ctgagccgcg cgcccctgca cttggggcat gggacgtggc tcatgaactc ttcgatcttc
     7561 tcccgcctgt actccgagtc ggtctcggcg tagcgccgct ccaggttgct cacgactccg
     7621 tcgaagcggg tcatgtactg cctgcggcgg ttgtagcggt tgcggtatga gacgtagatg
     7681 cgctcgccgt ccgtcccgaa gaggatgagg tgcctgtgct cctcgggcag gtcgcgccac
     7741 ggcacgtcca ggtcgatacc gaacttctcc gatagtgcgc cgagcacgct cctgtggtac
     7801 tcggtctgcg tgttggccca gggcacaacg gcaccttcgt taacgctcag atcctcgttc
     7861 gggactacga gcgcgggatc tatctccaga cggctcccga gcccgtcaca gcggtcgcac
     7921 gccccgtgcg ggctgttgaa cgagaacgtg cgcggctgta tctccgcgat cgaagctccg
     7981 cagttcgtgc acgtgaagct ctccgagaag agcatcgact ctcccg
//
LOCUS       DATAIR010000042         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_279278, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000042 DATAIR010000000
VERSION     DATAIR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279278"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VHM69_01115"
     CDS             <1..679
                     /locus_tag="VHM69_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2739016.1"
                     /translation="PFPYRLSPRTLIQAVDNSLERLGISTIDLYQIHWPFPWPRPKGL
                     MDALAEVVKEGKVRKVGVSNYGADATRRAHDRLASHGVQLASNQVQYSLLSRAPETNG
                     VLDACRDLGVTLIAYSPIAQGLLTGKYGPGAGRPAGFIRRFGRAFGEQNLRKVEPVVN
                     ILREIGSSQGKEPSQVALNWLIGQGTLPIPGAKNERQARQNAGAVGWSMTDEERERLN
                     LATLGWR"
     gene            676..>1657
                     /gene="kynU"
                     /locus_tag="VHM69_01120"
     CDS             676..>1657
                     /gene="kynU"
                     /locus_tag="VHM69_01120"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX2739017.1"
                     /translation="MTREEELAIRLDAADELARFREEFHLGAGIYLDGNSLGLLSHRA
                     ERTLSGVLTSWRERGIDGWTAGDHPWFYLAEEVGAMTAPLVGAAPDEVGATGGTTVNV
                     HQILATFFRPAGNRTKILATELDFPSDIQALTSQLRLRGLDPDKHLIRVKGRDGRTID
                     EEDVVAAMTDDVALVFLPTVLYRSGQLLGVGRLTRVAHERGIPIGFDTAHSVGVVPHS
                     FSGWDVDFAVWCGYKYLNGGPGAAAGLYVNRRRFGQRPGLAGWFGSRKDRQFDMDHDL
                     IPAKSAGAYHIGTPNILSLAPLLGSLEMFDEAGIGRVREKSLNLTGYLMDL"
BASE COUNT          309 a          557 c          522 g          269 t
ORIGIN      
        1 gccatttccg taccgcctga gcccgcgcac cctcatacag gccgtggaca atagtctgga
       61 gcgtctcggc atctccacga ttgacctgta ccagatccac tggcccttcc catggccgcg
      121 cccgaagggc ctgatggatg ccctcgcaga ggtggtgaag gagggcaagg tccgaaaggt
      181 cggggtaagc aactacggcg cggacgccac ccgccgcgcc cacgaccggc tcgcctccca
      241 cggcgtccag ctcgcctcca accaggtgca gtacagcctg ctcagtcgcg cgccagagac
      301 gaacggcgtc ctcgacgcgt gccgggatct cggcgtcacc ctgatcgcct acagccccat
      361 cgcccagggc ttgttgaccg gaaagtacgg accgggagcg ggaagaccgg ctggctttat
      421 ccgcaggttc ggacgcgcct tcggcgagca gaacctgcgc aaggtcgagc ccgtcgttaa
      481 tatcctgcgc gagatcggct cgtcccaagg caaagagcct tcccaggtag ccctgaactg
      541 gctgatcgga caggggacgc tacccatccc aggggccaag aacgagcgtc aggcccgcca
      601 gaacgctggc gccgtcggat ggtccatgac ggacgaggag cgggagaggc tgaacctggc
      661 aacccttggc tggcgttgac gcgggaagag gaactcgcaa taaggctcga tgctgcggac
      721 gagctcgccc gtttccggga agagtttcat ctcggggccg ggatctacct cgacggaaac
      781 tccctcggcc tgctttccca tcgggcggag cggacgctct caggagtgct gacttcctgg
      841 cgggagcggg gcatagacgg ctggaccgca ggagaccatc cgtggttcta cctggccgaa
      901 gaagtagggg ccatgaccgc accgctcgtc ggggccgcgc cggacgaggt aggcgccacc
      961 ggcgggacta ccgtgaacgt acaccagatc ctcgccacct tctttcgacc cgccgggaac
     1021 cggaccaaga tactcgccac cgaactcgat ttcccatctg atattcaagc cctcacgagc
     1081 cagctccgcc tgcgcggcct ggacccggat aaacatctca tccgggtgaa gggccgcgac
     1141 ggacgcacga tagacgaaga agacgtagtc gcggccatga ccgacgatgt cgcccttgtc
     1201 ttcctgccga ccgtcctcta ccgcagcggc cagttgctcg gcgtgggacg gctcaccagg
     1261 gtcgcccacg aacgcggcat ccccataggc ttcgataccg cgcactccgt cggcgtagtt
     1321 ccgcactcgt tctccggctg ggacgtggac ttcgccgtgt ggtgcgggta caagtatctg
     1381 aacggcggtc ccggcgcggc tgcggggctt tacgtgaacc ggaggcgctt cggccagcgt
     1441 ccagggctcg ccggatggtt cggctcccgc aaggacaggc agttcgatat ggaccacgac
     1501 ctcatcccgg caaaatcggc tggcgcgtac cacatcggca cgccaaacat cctcagcctc
     1561 gcgccactcc ttggctcgct ggagatgttc gacgaggctg gtatcgggcg tgttcgagag
     1621 aaatccctca acctcaccgg atacctgatg gacctcg
//
LOCUS       DATAIR010000043         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_279353, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000043 DATAIR010000000
VERSION     DATAIR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279353"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHM69_01125"
     CDS             <1..230
                     /locus_tag="VHM69_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX2739018.1"
                     /translation="GRTVTLLPPTYVAVVPVERILRTVPEAIGKYADDAAGAVPANVC
                     FHTGPSRSGDIEMSLFVGMHGPGDVHVVLVG"
     gene            complement(276..710)
                     /locus_tag="VHM69_01130"
     CDS             complement(276..710)
                     /locus_tag="VHM69_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2739019.1"
                     /translation="MSIFPTKILLATDGSKDAELALSTSVDLADSTNSELHVVTVAPG
                     YPSYDVRRPEVVEQLREQAEEILEDQVKKIEQKGGKVTEKHLRIAGRHRADQIVEVAE
                     EKEVGLIVMGSRGLGGVRRALMGSVSDSVVRHAHCPVLIVRE"
     gene            complement(931..2403)
                     /locus_tag="VHM69_01135"
     CDS             complement(931..2403)
                     /locus_tag="VHM69_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2739020.1"
                     /translation="MTESVSHKASADGWNEPTKNLPPLEPREMPEPLPLRKLIGASVI
                     LLATALGSGELLIWPYITTQAGVGLLWLATVGFMSQYFLNMEIERYTLATGETAVTGF
                     SRFWKPWGIVFALGAILPNAFPGWAASAATSLTFLTGWGESTIPWLVTILLLSIGLAI
                     TVSPVVYNTIERVEMVLVVIIVAFLGVAIVIATDLSAWTGIVTEAPEGITNMPGYISE
                     IGIATILGAIAFAGAGGANNLSQSNYIRDKGMGMGVHIPRIVSPITGEEEARPSTGYS
                     WEVNEENMRRWNGWWKVANREQLLTFVVIGLGTLIGLSVLVASVLGEGDYGDPGSIDF
                     LKEEAKVLGEQFGLWFEIFFYIAGFAILFSTNIGIVDYVSRLTADSLKTGYLAESQFW
                     SESKVYFVVAWIMCIGGSVIIWSGIEPLFLLVIASSGGGVVMFLYSGMLIWLNRRLLP
                     EPIRIKGARLIVVALTFAMFAVLSTYLVYYYVNAALTGQL"
     gene            2544..>2800
                     /locus_tag="VHM69_01140"
     CDS             2544..>2800
                     /locus_tag="VHM69_01140"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX2739021.1"
                     /translation="MSQVQVAIIADDLTGAADTGVQFARAGYQSAVFFRPAEVLPDDL
                     DAVAFDTDSRTMPEGFAAKRVVDAAHSARGARIVYKKLDSTL"
BASE COUNT          541 a          876 c          827 g          556 t
ORIGIN      
        1 agggccgcac ggttacgcta ctgccgccga cctacgtggc cgtcgtcccc gtggagagga
       61 tcctccggac cgtgcccgag gccataggaa agtacgccga cgacgcagcg ggagccgtgc
      121 cagccaacgt ctgcttccac acgggcccga gccgctccgg ggatatcgag atgtcgcttt
      181 tcgtagggat gcacgggccc ggggatgtac atgtcgtcct ggtgggttag gcaaagcccg
      241 gccgcttgaa gtgtggtcgg gctttgccta ttctcctact cgcgcactat cagaacggga
      301 cagtgggcgt gcctgaccac cgagtctgag acgctgccca tcagcgcacg cctgacaccg
      361 ccgaggccac ggctgcccat gacgattagg ccaacttctt tctcctccgc gacctctacg
      421 atctgatcag cccgatgccg tcctgctatg cgcaggtgct tctcggtaac cttgccgccc
      481 ttctgctcga tcttcttgac ctgatcttcg aggatctcct ccgcttgctc gcgaagttgt
      541 tctacgacct cgggcctacg aacgtcgtag gatggatagc caggcgcaac ggtaaccacg
      601 tgcaactccg agttggtgct gtcggccaga tccacggaag tgctgagggc cagctcagcg
      661 tccttggagc cgtcagttgc cagcagtatc ttcgtcggga atatgctcat ctcttcacgc
      721 ctcctcgatc gctttcccta cctggttatt agcataaatt agcacaatcg tcaaaggtct
      781 cgggtcgctt gcgcgtctac gcgagcaacc cgagcagata gactatcgtt agcaggacga
      841 agaagatgat catggccagc aggatggtta tccggagccg cttgaacacc ctggctgcgt
      901 cctatcaccc gttaattctc gccggtcccc ctatagttgt ccggtcaggg ctgcgttgac
      961 gtagtagtag accaggtacg tcgagagcac cgcgaacatc gcgaacgtta gagcaaccac
     1021 tatcaggcgg gcgcctttga tcctgatggg ctcaggcagg aggcggcggt tgagccagat
     1081 gagcatcccc gagtaaagga acatcacgac cccgccgccc gaggaagcga tgaccagcaa
     1141 gaagagtggc tcgatgccag accatatgat tactgatccc ccgatgcaca tgatccacgc
     1201 gaccacgaag tagaccttgc tctcgctcca gaactgactc tcggccaggt agcccgtctt
     1261 gagcgagtcg gcggtgagcc tcgagacgta gtcgacgatg ccgatgttgg tcgagaacag
     1321 gatcgcgaat ccggctatgt agaagaatat ctcgaaccag agcccaaatt gctcaccgag
     1381 gaccttcgcc tcttccttca agaaatcgat gctaccggga tctccgtagt ctccttcgcc
     1441 caggaccgag gcgacgagca cggagaggcc gatgagcgtt cccagcccta tgaccacgaa
     1501 ggtcaggagc tgctcccggt tggcgacctt ccaccagccg ttccagcggc gcatgttctc
     1561 ctcgtttacc tcccaggagt acccggtgga cggcctcgcc tcctcttctc ctgtgattgg
     1621 tgagacgatg cgggggatat ggactcccat gcccataccc ttgtccctga tgtagttgct
     1681 ctgggagagg ttgttggcgc cgcctgcgcc ggcgaaggct atcgccccca ggatcgtcgc
     1741 gatcccgatc tcgcttatgt acccgggcat attggtgatg ccctcggggg cctcggtgac
     1801 gatgccagtc caggctgaca gatcggtggc tatgactatc gccactccca ggaaggctac
     1861 gatgatgacg acgagcacca tctcgaccct ctcgatggtg ttgtagacga caggagagac
     1921 ggttatcgcg aggccgatgg agaggagcaa aatcgtgacg agccagggaa tggtgctctc
     1981 accccagccg gtgaggaagg tcagcgacgt ggccgcgctc gccgcccagc ctgggaaagc
     2041 gttgggcagg atcgcgccca gagcgaagac tatcccccag ggtttccaga agcgggaaaa
     2101 gccggtcacg gcggtctcgc cggtcgccag ggtgtagcgt tcgatctcca tgttgaggaa
     2161 gtactgcgac atgaaaccaa cggtcgccag ccacagcagt cctacccccg cctgcgtggt
     2221 gatgtagggc cagatcagca actcgccgga accgagcgcc gtggcgagca gaatgacgct
     2281 cgcccctatg agcttgcgta gtggcaaggg ctccggcatc tctcttggct ctagcggcgg
     2341 caggttcttg gtcggttcgt tccagccgtc ggcagacgcc ttgtggctca cggattcggt
     2401 catgtataaa ccctccttgg tcccttttct agcccccggt acctgtgagc atgttattga
     2461 aatgctaacg gcgtgtcaac agcggtagat ttcctacgag cccgaagaga tgctatttta
     2521 agggcgttcc cgacgctctc cgggtgagtc aagtgcaggt agccataatc gccgacgacc
     2581 tgaccggcgc cgcggacacc ggcgtccagt tcgcccgcgc cggttaccag agcgccgtgt
     2641 tcttccgccc ggcggaggta ctccccgacg acctcgacgc cgtagccttc gacactgact
     2701 cccgcaccat gccagagggc ttcgcggcga agcgcgtcgt cgatgccgcg cactcagccc
     2761 gtggggctcg catcgtctac aagaagctcg actccaccct
//
LOCUS       DATAIR010000044        11296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94664, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000044 DATAIR010000000
VERSION     DATAIR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94664"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VHM69_01145"
     CDS             complement(<1..347)
                     /locus_tag="VHM69_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739022.1"
                     /translation="MLLAAGVAVATTGAERLVVGYNVIMGTHGANDLRGGPGADLMAS
                     EGTWGASPEDTALDTGNGAGGDDDLIDPVSDPPSRDVERCSPGRDEVQADPEDDVGED
                     CETVQIIDASKGRG"
     gene            complement(595..1128)
                     /locus_tag="VHM69_01150"
     CDS             complement(595..1128)
                     /locus_tag="VHM69_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739023.1"
                     /translation="MATTTMTKTYFEDLGRIAESNAKTAQESARIATDYWGASRESNF
                     KLAKDMTRTITEGMKRQSNANEELTSRIFELLEERDEAHKRFFGQWAEAFTTVPFDFA
                     RQATREAHKSANSVTASVNGGFPIARYDELSVEEISGRLDSLTDAQIKQVRDHERRTK
                     NRKSLMEQFERKLKVVS"
     gene            complement(1520..1720)
                     /locus_tag="VHM69_01155"
     CDS             complement(1520..1720)
                     /locus_tag="VHM69_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739024.1"
                     /translation="MTMTITHEKTREVTREDVDRAIDVIERHLEKEARTRNLLDEQAI
                     MRPKDLCKQQGLDMVAELRGMM"
     gene            complement(1862..2569)
                     /locus_tag="VHM69_01160"
     CDS             complement(1862..2569)
                     /locus_tag="VHM69_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2739025.1"
                     /translation="MIARYAGGESGARAAARAGSVVVVVDAFRASTTISVLVAKGARV
                     VPVGSIERAYSYAADLRIGERGSAKVAGFDFGNSPTEVLEAAVPPNCTVALSTTNGTR
                     VVEAARGARAILTGAFINAGALADELSGGTYEVVVVGCGWKGRRASEDEAAAGAILHR
                     LRSKGAELDQRAGRVVEDYLSRSVKTLRNNSAAKRLKSLGYERDLEFCLAEDTVPVVP
                     RLVGDAFVGGAAVSGRP"
     gene            2694..3704
                     /locus_tag="VHM69_01165"
     CDS             2694..3704
                     /locus_tag="VHM69_01165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2739026.1"
                     /translation="MRYRNMGGTDIEVSEVGFGVWTVSTGWWGEVDDERSIRLMREAR
                     EQGINYFDTADTYGSGKGETLLADAFGHMRDDVVISTKIGYDFYNHTARRGQQERPQD
                     WSEEFIRFALEQSLKRLDTDYIDFLQLHNTKMDAIDDEDLFALLEEFKGEGKVRAYGI
                     ALGPKIGWLEEGVRAMRQRRIDGVQMIYNLLEQDPGRGLIEAARDTETALIVRVPHSS
                     GMLEGHYDENTTFAKNDHRRHRPKEWLTTGLKKVEQLSFLTESGERTLGQAALKFALA
                     SPEVISTLPNIYGEEQIEEFAAAPNTPDLTPEELSRVAELYEENFGLEASDDRLQASG
                     KG"
     gene            3840..4562
                     /locus_tag="VHM69_01170"
     CDS             3840..4562
                     /locus_tag="VHM69_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX2739027.1"
                     /translation="MTETRTRQQQAQEAALPAQYINYVFFKLDPMWRRLPDEERERGR
                     QEFRTVAQEHAGEMLLRSFSLMGLRADADFMLWRIGYDLDAFESMTAAMLKSGLGKYL
                     RVSYSYFALSRRSIYVGEHTPGFENRTYIVPGEGEYLFVYPFVKTRAWYRLPIEERQR
                     MMNEHMKIGRKHYPVKNNTGYCFGIDDYEFILSFEAESPQKFQDLMMELRESEASAYT
                     ELDTPIFTCRRRALPEILETLG"
     gene            4755..5654
                     /locus_tag="VHM69_01175"
     CDS             4755..5654
                     /locus_tag="VHM69_01175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX2739028.1"
                     /translation="MGTNVTELVGETPLLELPNISAEVPDVRIFGKAEWYNPGGSVKD
                     RPALWMIRDGEKTGALTPEKTILDATSGNTGIAYAWIGAALGYRVKLCMPKNASEERK
                     KILRAYGVEFVLTDAGEGSDGAIREARRLYAEAPELYFYPDQYSNPANPRSHYESTAP
                     EIWEQTGGEVTHFVAGLGTSGTFVGTAKRLKEYNPEIEAISFEPDSAFHGLEGMKHME
                     SAIVPSIYDPTIADQNRSVRTEDAYDMVKRVAREEGILIGISAGAAVATALRVAKEIG
                     SGTIVTILCDGADKYLSESFWEG"
     gene            5656..6075
                     /locus_tag="VHM69_01180"
     CDS             5656..6075
                     /locus_tag="VHM69_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2739029.1"
                     /translation="MPLKIGAGDVGQIHYHARETYPEECAGALVGIDNGGIKVVVDVW
                     RAENTHEEERSRRFLIEPLTIKKFEEQAEERDMGLLGFYHSHPDHPAEPSEYDREHAW
                     PGYSYVIASVSGENVEDMRSWTLRDDRSGYDEEPIVG"
     gene            6092..6385
                     /locus_tag="VHM69_01185"
     CDS             6092..6385
                     /locus_tag="VHM69_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2739030.1"
                     /translation="MPKVKIPTPLRQYTGGNTEVEVGGDTVGEALGSLASEHPDLKQH
                     LYTGDGQLRSFVNVYKGDEDIRYLEGQDTNVAEDDELSIIPSIAGGSAEGRCK"
     gene            6481..7623
                     /gene="moeB"
                     /locus_tag="VHM69_01190"
     CDS             6481..7623
                     /gene="moeB"
                     /locus_tag="VHM69_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX2739031.1"
                     /translation="MEFSNEEIARYSRHLIMPEVALEGQKKLKAAKVLTVGTGGLGSP
                     LALYLAAAGVGTIGIVDFDVVDESNLQRQIIHGTSDVGRPKVESAYDKLKDINPNVEV
                     RVHEEALTSENAFEIFEDYDVIVDGTDNFPTRYLVNDACVLLNKPNVYGSIFRFEGQA
                     SVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQATETVKLLLGIGEP
                     LIGRLLLYDALGMSFREMKLRKDPNCPVCGENPTVTELIDYQEFCGIPQANAAEQANG
                     VPEITVNELKHKLDNSEDINVLDVREPHEYEVANIGVRLIPLGELPQRLAELEQDDIL
                     AIHCKTGARSARAVKLLQDAGFENVYNVKGGITAWSEEIDPSIPKY"
     gene            8077..8472
                     /locus_tag="VHM69_01195"
     CDS             8077..8472
                     /locus_tag="VHM69_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX2739032.1"
                     /translation="MGEVEALLDKAQRSFAAATRLLDAGDTDFGTSRAYYGYFYVAEA
                     MLLSKGLTFSRHAQVISQYGLHFARNEVLDRRFHKRLDDAFSLRQLADYSTDTSLEPD
                     TIRDLIEEGDIFLHDARGYLARRPSGEHS"
     gene            8486..9613
                     /locus_tag="VHM69_01200"
     CDS             8486..9613
                     /locus_tag="VHM69_01200"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2739033.1"
                     /translation="MRHAQSAKTGKILQYAVCIIWRMDTIPIFDGHNDTLLNLHLPDR
                     GEGRTFFERSERGHIDLPRAREGGFGGGFFACYVPNPEDDGWTEESALEITEDGYEVS
                     DAPALDLTYARDFADKLVVGLFELESAGGLKVVRTADELEGCLGDGTIAAILHFEGAE
                     NLGPDAGALEDLYKVGLRSLGLVWSRPNAYAHGVPFRFPASPDTGPGLTGAGKELVRE
                     CNRLGVLIDLSHLNESGFWDVAELSEAPLVATHSNAHALCPASRNLTDRQLDAIKDSD
                     GMVGVNFAVGFLREDGHDSEETPLETVVRHVDYLVERAGIERVGFGSDFDGAKVPMEI
                     GDVSGLPKFLTVLRGHGYDEAALKKLAHGNWIRVLHKTWHQ"
     gene            complement(9676..10599)
                     /locus_tag="VHM69_01205"
     CDS             complement(9676..10599)
                     /locus_tag="VHM69_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEX2739034.1"
                     /translation="MEDLESSIEAVRRFNRVFTRRIGVLREGLLHSSYSLAEARVLFE
                     LAHREDTSASDLARELGLDPGYLSRTLARLEEQGLVARARSERDGRRRDISLTSTGED
                     AFEMLDSRSREEVAEMLGGLSEEDRRRLLEAMGTIEGTLQKDFKFSEPFVLRAHEPGD
                     MGWVVHRHGVLYAREYGWDERFEALVARIVADFIEGYDPAKERCWIAEMGGKQVGCVF
                     VVKQSETVAKLRLLLVEPDARGLGLGSRLVGECIRFARNRGYQKITLWTNSVLDAAWH
                     IYEEHGFELVDEEEHHSFSRDLVGQNWELAL"
     gene            complement(10665..>11296)
                     /locus_tag="VHM69_01210"
     CDS             complement(10665..>11296)
                     /locus_tag="VHM69_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2739035.1"
                     /translation="ADAEVLSRGLDDKNSDSRELVDDLLGETDRMNALLSDLLLLARL
                     DADKVSVSREPFDLALVLSETAERFAARAAAEGKNLEVHHSGKLPARGDAVRTGQILA
                     ALIDNALRFTPAGGTITVEGRTKDRRVEATVMDTGPGIAPENLNRIFERFFRADTHSA
                     ARTRGASGGGTGLGLAIARDLARAQGGRLVAGNAAGGGATLTLLLPSGP"
BASE COUNT         2265 a         3538 c         3446 g         2047 t
ORIGIN      
        1 cctcgccctt tcgatgcgtc tatgatctga accgtttcgc agtcttcgcc tacgtcgtct
       61 tcggggtccg cctgcacctc gtctctgccg ggactacagc gttcgacgtc cctggatggc
      121 gggtcgctca ccgggtcaat gaggtcgtcg tcgccgcctg ccccgttgcc ggtatccagg
      181 gccgtatcct ccggcgaagc accccaagtg ccctcggagg ccataaggtc ggcgcctggt
      241 ccgccacgca ggtcgttggc gccgtgggtg cccatgatca cgttgtagcc cacaaccagg
      301 cgttcggctc cggtagtggc aacggcgacc cctgcggcca gcagcaatac acaagccgtg
      361 gcggccagga agaacagctt cctcttcgtg agtatcgcag cctcctcgac gcactcagcc
      421 tgatttgcat tatgagtagt ttaaggcatc cgggagcgca agcgatgaag tggcttcgga
      481 gaagcccgtg acgcgaacgt cggagacaac accttccacg cgatcgcgtg aatagggcat
      541 gagaaagggc tgggtccaat gacccagccc ttcacttctg cttcagcctt actgttacga
      601 gactactttg agcttgcgct cgaactgctc cataaggctc ttgcggttct tagtacgccg
      661 ctcgtggtcc cgcacctgct ttatctgagc gtcggtgagg gagtcgagcc gccccgagat
      721 ctcttccacg ctcaactcgt cgtagcgagc gatggggaag cccccgttta cggacgccgt
      781 gacgctgttg gcgctcttgt gcgcctcgcg cgtggcctgc cgggcaaagt cgaatggcac
      841 agtggtgaac gcttcggccc actgcccgaa gaagcgcttg tgggcttcgt cgcgttcctc
      901 aaggagctcg aagatcctgc tcgtaagctc ctcgttggcg ttcgactgac gcttcatgcc
      961 ctcggtaatg gtccgagtca tgtccttggc gagcttgaag ttgctctcgc gggacgcgcc
     1021 ccagtagtca gtcgctattc gggcgctctc ctgggcggtc ttcgcgttgc tctcggcgat
     1081 cctgcccagg tcctcgaaat acgtcttggt catcgtggtc gtggccactt tcgacacctc
     1141 cgtcggtcgt ggtctcgtcc agttccagat atatacctac cgtgcacccg gcgcagctaa
     1201 acatccccgt agcaccttcg cgtgactgcg cggattcgcc aggctgagta gcgcagcaaa
     1261 aggcgaatta caccagccct gccccgaacg acgatgaact gtcgataacg ccacaatcga
     1321 gaatcaggct aaggtaacga agacctcaag aaatcgggaa cactatcgct cgacttcgaa
     1381 gcgcgcaaga aaagagggcc ggagagatcc gaccctcggc caggctcgtg aagggggatg
     1441 gacagccctc aattgtctac atgagagacc gggggtttta ctgtgcatcc cccttcatgg
     1501 gccctgaata gtcgcgcgtt cacatcatgc cgcgcaactc ggcaaccata tccaggccct
     1561 gctgcttgca caggtccttg gggcgcatga tcgcctgctc gtccagcagg ttgcgggtcc
     1621 tggcctcttt ctcgagatga cgctcgatga cgtctatggc ccgatccacg tcctcgcggg
     1681 taacctcgcg ggtcttctcg tgtgtgatgg tcatagtcac tgtatcttca cctcctttcc
     1741 ctagcggatt cgacggagct tgtaacattt gatccgccgt cgctgactcc agagtaatgg
     1801 tgcgccaccc ccttgtcaag aagaattttg ggcttttctc ttggccccag ggccgaagat
     1861 ttcaggggcg cccggatacc gcggcacccc cgacgaaggc atctccgacg agacgcggca
     1921 cgacgggcac ggtgtcctct gcgaggcaaa actccaggtc ccgctcgtac ccgagacttt
     1981 tgagcctctt ggcggcgctg ttgttgcgca gcgtcttcac ggaacgggac agataatcct
     2041 cgacgacgcg gccggcccgc tggtccagtt cagcaccctt agaccgcagc ctatggagga
     2101 tggccccggc cgctgcctcg tcctcggagg cgcggcggcc cttccagccg cacccgacca
     2161 ccacgacttc gtaagtcccg ccggacagct cgtcggccag agcgccggcg ttgatgaagg
     2221 cgccggtcag gattgccctc gcgccgcggg cggcctcgac gacgcgggtc ccgttcgtcg
     2281 tcgatagcgc gaccgtgcaa ttcgggggca ccgcggcttc caggacctcc gtcggcgagt
     2341 tgccgaagtc gaagcctgcg accttcgcgc tgccccgctc cccgatacgg aggtcggcag
     2401 cataggagta agccctctct atggaaccga cgggcacgac cctggcgccc ttcgcgacga
     2461 ggacggagat ggtcgtgctc gccctgaagg cgtcaacgac gaccaccacc gagccggcgc
     2521 gggcggcggc ccgcgcaccg ctttcccctc ctgcataacg tgcgatcatg gcgcgagacc
     2581 tttggctttc cggaacatcg ggcgattata gccttgcgga ttgagagaaa gtcaacagcg
     2641 accggcgtta gaatgtagta attactaccc gaagactcga ataggagtag aagatgagat
     2701 atcggaatat gggcggcacg gatatagagg tctccgaggt cggtttcggg gtgtggacgg
     2761 tctcgaccgg ctggtggggc gaggtggatg acgagagatc cataagactc atgagagagg
     2821 ctcgtgagca gggcatcaac tacttcgaca cggcggacac ctacggatcc ggtaaaggcg
     2881 agacgcttct ggccgacgcc ttcgggcaca tgcgcgacga cgtggtgatc tcgacgaaga
     2941 taggctacga cttctataac cacacggccc gccgcggcca gcaggagcgc ccgcaggact
     3001 ggtccgagga gttcatccgc ttcgccctgg agcagagcct gaagaggctg gataccgact
     3061 acatagactt cctccagctc cataacacga agatggacgc catcgacgac gaagatctct
     3121 tcgccctgct ggaggagttc aagggcgagg gcaaggtcag ggcctacggc atcgccctcg
     3181 ggcccaagat cgggtggctc gaagaaggcg tcagggccat gcgccagcgc aggatagacg
     3241 gggtccagat gatctacaac ctcctcgaac aggaccctgg ccggggcctg atcgaggccg
     3301 cccgcgacac ggagacggcc ctgatcgtac gcgtccccca ctcctcaggg atgctcgaag
     3361 gccactacga cgagaacacg acgttcgcca agaacgacca ccgccgccac cgcccgaaag
     3421 agtggctcac caccggcctg aagaaggtgg aacaactctc cttcctcacc gagtcgggcg
     3481 agcgcaccct cggccaggcc gccctcaagt tcgccctcgc atcccccgaa gtcatctcca
     3541 ccctccccaa catctacggc gaggaacaga tagaagagtt cgccgcggcc ccgaataccc
     3601 ccgacctcac cccggaggag ctctcccgag tagccgaact gtacgaggag aacttcgggt
     3661 tggaggcttc agatgacagg cttcaggctt caggaaaggg ataaggaaca cgctccagtc
     3721 aggcgccacc atgcacggac cagggctcta tacaggaaaa cgcaacgccg cgcgaagagc
     3781 gtacgccatc aaaaagctga aacctgatac ctgaagcctc tcgaccgaag ggagagaaaa
     3841 tgaccgagac ccgaacccgc cagcagcagg cgcaggaagc ggctcttcct gcccagtaca
     3901 taaactacgt cttcttcaag ctggacccta tgtggcgccg gcttcctgac gaggagcgcg
     3961 agcggggcag gcaggagttc cggacagtag cgcaggagca cgcgggggag atgttgctga
     4021 gatccttctc tctcatgggg ctacgcgccg acgcggactt catgctgtgg aggatcggct
     4081 acgacctcga cgccttcgag agtatgaccg ccgccatgtt gaagtcgggc ctcgggaagt
     4141 acctgcgcgt ctcctactcg tacttcgcgc tgagcagacg ctccatctac gttggggagc
     4201 acacgccggg tttcgagaac cgcacgtata tcgtccctgg cgagggcgaa tacctcttcg
     4261 tctatccttt tgtcaagacg cgggcctggt accggttgcc catcgaagaa cgccagcgaa
     4321 tgatgaacga gcacatgaaa ataggccgca agcactatcc cgtaaagaac aacacaggct
     4381 actgcttcgg catagacgac tacgagttca tcctatcctt cgaagccgag tccccccaga
     4441 agttccagga cctgatgatg gagctcaggg agagcgaagc aagcgcctac accgagctcg
     4501 acacccctat cttcacctgc cgccgccgcg cgctgccgga aatcctggag actttgggat
     4561 aatttgcgct cgcaccgtag aatcgcgacg ccaggttcga gtataatccc gcgcaacgca
     4621 ccaaccatgg ccgggaggct cctatcttgc ctctcggcta tcatgccggt caggcatccc
     4681 gactcgcttg tctggacgcc gaaccttcac cctgtataat taatcccgac atgatcactc
     4741 ggattaatga gcggatgggg actaacgtga cggagctggt cggcgagacg ccgctcctgg
     4801 agttgccgaa catctcggct gaagttccag acgtccgcat cttcggcaag gcggaatggt
     4861 acaacccggg cgggtctgta aaagaccgtc ctgctctgtg gatgatcagg gacggcgaga
     4921 agacgggggc tctgacgccg gagaagacca tcctcgacgc gacgagcggg aacactggca
     4981 tagcgtacgc ctggatcggg gccgcgctcg gctacagggt gaagctgtgc atgccgaaga
     5041 acgccagcga agagcggaag aagatcctgc gtgcctacgg cgtggagttc gtgttgacgg
     5101 acgccggcga gggatccgac ggtgccatca gggaggcgcg gaggctctac gccgaggctc
     5161 ctgagctgta cttctacccc gaccagtact ccaacccggc caacccgcgc tcgcactacg
     5221 aatcgacggc gccggagatc tgggagcaga cgggcggcga ggtcacgcac ttcgtggctg
     5281 gcctgggcac gagcggcacc ttcgtcggca cggcgaagcg gctcaaggag tacaaccctg
     5341 agatagaggc catctccttc gaaccagact cggccttcca cggcctcgaa ggcatgaagc
     5401 acatggagag cgccatcgtg ccctccatct acgacccgac catcgcagac cagaacaggt
     5461 cggtgaggac ggaggacgcc tacgatatgg tcaagcgggt ggcgagggag gaaggaatcc
     5521 tcatcggcat ctcggccggg gcggcggtcg cgacggcgct cagggtggcg aaggaaatag
     5581 gttcagggac gatcgtcacc attctgtgcg acggggctga caagtacctc tccgagagct
     5641 tctgggaggg gtaggttgcc gctcaagata ggcgccgggg atgtcggcca gatccactac
     5701 cacgcgaggg aaacgtaccc cgaggagtgc gccggcgcgc tggtcgggat cgacaacggc
     5761 ggtatcaagg tcgtcgtcga cgtgtggagg gccgagaaca cgcacgagga ggagcgcagc
     5821 cgccggttcc tcatagagcc gctcacaata aagaagttcg aggagcaggc ggaggagcgg
     5881 gacatggggc tgctcggctt ctaccactcc caccccgacc atcccgccga accttccgaa
     5941 tacgatcgcg agcacgcatg gcccggctac tcgtacgtga tagcctcggt gagcggtgag
     6001 aacgtcgaag acatgcgctc gtggaccctc agagacgacc gctcgggcta cgacgaagag
     6061 ccgatagtag gataagagaa ggagcttaga tatgccgaag gtcaagatcc caaccccctt
     6121 gaggcagtac acaggtggca acacagaggt cgaagtcggc ggcgacactg tgggcgaggc
     6181 cctcgggagc ctcgcctcag agcaccccga cctcaagcag cacctctaca ccggcgacgg
     6241 ccagctccgc tcgttcgtca acgtctacaa gggcgacgag gacatccgct acctcgaagg
     6301 tcaggacaca aatgtcgccg aggacgacga gctctccatc atcccctcca tcgcgggagg
     6361 ctccgcagag ggacgatgta agtagttttc gaacgctcat tcttcaatcc catttcttag
     6421 aacaagaggt tttttcagat gcagacaaga cagcccttag tagagtcacc caacgggtcg
     6481 atcgagttca gcaatgagga gatcgcacgc tacagcaggc acctcattat gcccgaggtc
     6541 gccctcgaag ggcagaagaa gctcaaggcg gcgaaggtcc tcacggtcgg cactggcggt
     6601 ttgggtagcc ctctggccct gtaccttgcg gcggccggcg tcgggaccat cgggatcgtc
     6661 gacttcgacg ttgtagacga gtccaacctg cagcgccaga tcatccacgg cacctccgac
     6721 gttgggcgtc ccaaggtcga gagcgcctac gacaagctca aggacatcaa cccgaacgtc
     6781 gaggtcaggg tacacgaaga ggctctgacg agcgagaacg cgttcgagat cttcgaggac
     6841 tacgacgtga tcgtcgacgg cacggacaac ttcccgaccc gctatctggt taacgatgcc
     6901 tgcgtcctcc tcaataagcc gaacgtctac ggctccatct tccgcttcga gggccaggcg
     6961 agcgtgttct acgccgagga aggcccttgt taccgctgcc tctacccaga gccgccgcca
     7021 cctggactcg tccctagctg cgcggagggc ggggtgctcg gcatcctgcc tggcgccatc
     7081 ggcaccatcc aggcgaccga gaccgtcaag ctcctgctcg ggatagggga gcctcttatt
     7141 ggacgtctcc tcctctacga cgccctcggc atgagcttcc gcgagatgaa gctccgcaag
     7201 gacccgaatt gccctgtctg cggcgagaac ccaaccgtta cggagctcat cgactaccag
     7261 gagttctgcg gcatcccgca ggccaacgcc gccgagcagg cgaacggtgt gcctgagatc
     7321 accgtgaacg agctcaagca caagctcgac aacagcgagg acatcaacgt gctcgacgtg
     7381 cgcgagccgc acgagtacga ggtcgccaat atcggcgtga ggctcatacc cctgggcgag
     7441 ctgccgcaac gcctggcgga gctggagcag gacgacatcc tcgccatcca ctgcaagacc
     7501 ggcgcccgca gcgcaagggc cgtcaaactt ttgcaggatg cgggctttga gaacgtctac
     7561 aatgtgaaag gcggcatcac agcctggagc gaggagatcg accccagcat cccgaagtac
     7621 tagctgaata tagtgttact ttgggcgggg gttgcgaatg caatccccgc ttttcgcatg
     7681 tccaggtatg cggacaacta attgcctcgg gtagaatgaa ggtatggatc ccgcgcgtgc
     7741 cagggtcata gagagtattc cggatgacct gagcgtactc ctggaccggc tggctctcga
     7801 actcaaggag ttgtatggtg gacagtaccg gggcatggtg ctctacggtt cttatgcacg
     7861 cggtgaggcc gacgagggca gcgacgtaga catactactg cttttggaag gcgaagtgaa
     7921 ttcgacccgc gagctactac gagcgtcaca ggtcaagtgg ccactctcac tggaagccgg
     7981 atacaccatc tcgctgttgc cagtgagcgt tgaggcctac caaaactccg aacaaccctt
     8041 tctccgcaac gcacggggag aaggcatttc ggtctcttgg gcgaggtcga agctctactg
     8101 gacaaagctc agcgctcctt cgcggcagcc acgcgattat tggacgcggg agacacggac
     8161 ttcggcacgt cgagagccta ctacggctac ttctacgtcg ccgaagctat gctgctctcg
     8221 aaagggttga ccttctcccg gcacgcacag gtcatctccc agtacgggct gcacttcgcc
     8281 aggaacgaag tcttggaccg ccgcttccac aaacgtctcg acgacgcctt ctctttgagg
     8341 caattggcgg attactccac agatacttct ctggaacccg atacgattcg tgatttgatc
     8401 gaagagggag atatctttct acacgacgcg cgaggatatc tcgcaaggcg gccatctgga
     8461 gagcactcct agcaggacgc ctcgggtgcg gcatgcccag agcgcgaaga cgggcaagat
     8521 actgcagtac gcagtctgta tcatctggcg gatggacacg atacccatct tcgacggcca
     8581 taatgacacc ctgctcaacc tgcatctccc ggatcgtggc gaagggcgca cctttttcga
     8641 gcgcagtgag cggggccaca tcgacctgcc gcgcgcccga gaaggcggct tcggtggcgg
     8701 gttcttcgct tgctacgtcc ccaaccccga agatgacggc tggaccgagg aatcggcgct
     8761 cgagatcacc gaggacggct acgaggtctc ggacgctccg gcgctggatc tcacctacgc
     8821 cagagacttc gccgataaac ttgtagttgg cctctttgag ctggagtccg cgggcggact
     8881 caaggtcgta cgtacggctg acgagctcga aggatgcctg ggcgacggga ccatagcggc
     8941 catcctgcac ttcgagggcg cggagaatct gggcccggac gcgggagcac tcgaagacct
     9001 ctacaaagtg ggcctccgct cgctcgggct cgtgtggagc aggcccaacg cttacgccca
     9061 cggcgtccct ttcaggtttc cggcctctcc cgacacgggt cctggcctta ccggagcagg
     9121 gaaagaactc gtcagggaat gtaaccggct tggagttctg atcgacctct cccacctgaa
     9181 cgaaagtggg ttttgggacg tcgcggagct ctccgaagca ccgctagttg cgacccactc
     9241 gaacgcccat gccctctgcc ccgcgtcgcg caacctgacg gacagacaac tcgacgccat
     9301 caaggactcg gacgggatgg ttggcgtgaa cttcgccgtc ggcttcctgc gcgaggacgg
     9361 tcacgattcc gaagaaaccc cgctggaaac ggtcgtgcgg cacgtggact atctcgtcga
     9421 gcgcgccggc atcgagcggg tagggttcgg ctcggacttc gacggggcga aggtgccaat
     9481 ggaaatcggg gacgtctccg gccttccgaa gttcctcaca gtcctgcgag gacacggcta
     9541 cgacgaagct gcattgaaga agctggcaca cgggaactgg atcagggtgc tgcacaagac
     9601 ctggcaccag taagttgcgg tctctttctt gccggctggc tcatctgact gcagaagatc
     9661 ccgtagcctc gatacttaca gcgcgagttc ccagttctgg ccgacgaggt cgcgactgaa
     9721 gctgtggtgc tcttcttcgt ccacgagctc gaagccgtgt tcctcgtaga tgtgccatgc
     9781 agcgtctagc acgctgttcg tccagagcgt gatcttctgg tatccccggt tccgggcgaa
     9841 gcggatgcac tctccgacga gccggctgcc aaggccgagt ccgcgcgcat cgggctcgac
     9901 tagcagaagc ctgagcttgg caactgtctc gctctgcttt accacgaaga cgcatcccac
     9961 ctgtttcccg cccatctcgg cgatccaaca ccgctccttc gcagggtcgt agccttcgat
    10021 gaagtccgcg acgatgcggg ccaccagtgc ctcgaatctc tcgtcccagc catactccct
    10081 cgcgtagagg acgccgtgcc gatggacgac ccaacccata tcccccggct cgtgcgcccg
    10141 caacacgaat ggctcggaga acttgaagtc cttctgcagt gtcccttcga tggtccccat
    10201 ggcctccagc agacgacgcc ggtcctcttc tgagagcccg ccgagcatct cagccacctc
    10261 ctcccgcgag cggctgtcga gcatttcgaa agcgtcctct ccggtagagg tcagggagat
    10321 gtcgcgccgg cgaccgtctc tctccgacct tgccctggcg acgagcccct gctcctcgag
    10381 acgtgccagc gtgcggctca gataccccgg gtccagcccc agctcgcgcg cgaggtcaga
    10441 ggcgctggta tcctcgcggt gcgccagctc gaacaggacg cgggcctccg ccagggagta
    10501 gctactgtgc aacagacctt cgcgtaaaac cccgatccga cgcgtgaaga cccggttgaa
    10561 gcgccggacc gcctctatgc tcgactccag atcctccaca tatcacctcc ctgtatgctt
    10621 gccattgtct attatttgat tgctatagtc aattataagt gggatcaggg accagagggc
    10681 aggaggagcg taagggtggc tccgcctcct gcggcgtttc cggctacgag cctgccgccc
    10741 tgggcgcggg ccaggtcgcg ggcgatggcg agaccgagac cggtaccgcc gccggaagct
    10801 cccctggtgc gtgcggcgct gtgcgtatcc gccctgaaga agcgctcgaa gatgcggttc
    10861 aggttttccg gggcgatgcc gggtcctgtg tccataacgg tcgcttcgac cctcctgtcc
    10921 ttggtgcggc cttctactgt tatggttccg ccggcgggcg tgaaccggag tgcattgtcg
    10981 ataagtgcgg ccaggatctg gcctgtacgg acagcatcgc cgcgcgcggg gagcttgccc
    11041 gagtggtgga cctcgaggtt tttgccttcg gcggcggcgc gggccgcgaa gcgctccgcg
    11101 gtctccgaga ggacgagggc gaggtcgaaa ggttcgcggg agacggagac cttatcagcg
    11161 tcgaggcgcg ccaggagcag gaggtcggag agcaaggcgt tcatcctatc cgtctcgccg
    11221 agcaggtcgt ctacgagctc gcggctgtcc gagttcttgt cgtcgaggcc acgcgagagg
    11281 acttcggcgt cggccc
//
LOCUS       DATAIR010000045         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_2473, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000045 DATAIR010000000
VERSION     DATAIR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VHM69_01215"
     CDS             complement(<1..525)
                     /locus_tag="VHM69_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739036.1"
                     /translation="MSPDLGFVHRFIPGKDDSAPTLLLLHGTGGNEEDLIPLGQDLAP
                     DAAILSPRGKVLEFGAPRFFRRLAEGVFDHEDLLFRTHDLAGFVEAASEEYGFDLSKL
                     VAVGYSNGANIAASTIFLHPGLLRAAVLFRAMVPFEPEVTPDLSGMPVFIAAGRRDRM
                     IPPDNAQRLADILRE"
     gene            complement(522..1472)
                     /locus_tag="VHM69_01220"
     CDS             complement(522..1472)
                     /locus_tag="VHM69_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX2739037.1"
                     /translation="MAPQTTGIHHVTAISGEPQRNVDFYAGILGMRLVKKTVNFDDPE
                     TYHLYYGDSAGTPGTIMTFFPWAHAPGGRIGAGQLVVTSFSIPATSLGYWTERLVEKG
                     VRFEKPRDRFGDTVLTLQDPDRLRIELVATEDGRAGWEGSIVPAEHTVRGFHHVALAV
                     ETTESTARLMTETLGFRQVDEAEGRIRLSSGEGGPGNLVDLVSAAGFPRGSMGVGTVH
                     HVAFRVPDDEIQLALREEISALGYNVTPVLDRNYFRSIYFREPGGVLFEIATDPPGFT
                     VDEPDEELGTHLKLPPWLETRRDRLEEVLPPLRVPAGENV"
     gene            1590..>2500
                     /locus_tag="VHM69_01225"
     CDS             1590..>2500
                     /locus_tag="VHM69_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739038.1"
                     /translation="MPLALEFCAALVAIVFAAVLFTNAVEILGGRLNLGAGAVGSVLA
                     AVGTALPETMIPVVAILAAAFAGEGGEAAGEIGIGAILGAPFLLSTLAMFVVGSSALA
                     YRGRREQGAKIRCSEASVGDEFPWCKTPAKEVIVDEKTIARDMGFFLIFFAVAAGAGI
                     VELPLALRFALAAILISAYALYVRRTLHSGGALEEVPQSLTLWPFASRPPTWIVVCQA
                     VLSLALIVVGAEFFVGAVEGAAEVVALPAGLVALILAPLATELPEKINSVLWVREGKD
                     TLALGNITGAMVFQSTVPVTLGVLFTP"
BASE COUNT          389 a          752 c          862 g          497 t
ORIGIN      
        1 ctcgcgcagg atgtcggcca gccgctgtgc gttgtcgggt gggatcatac ggtcccggcg
       61 ccccgctgca atgaagacgg gcatccctga gaggtcaggc gtcacttcag gctcgaaagg
      121 caccatcgcc ctgaacagga ctgcggcccg caacagaccg gggtgcagaa aaatggtgct
      181 cgcggctatg ttggctccgt tggagtagcc taccgcgacg agcttcgaga ggtcgaagcc
      241 gtattcctcg gatgcagcct cgacgaaccc ggcgagatcg tgggtgcgga agaggaggtc
      301 ttcgtggtcg aagacgcctt ctgcgaggcg tcggaagaag cggggggcgc cgaactccag
      361 gaccttgcct cggggggaga ggatggctgc gtccggggcg agatcctgtc cgagagggat
      421 caggtcttcc tcgtttcctc ccgtgccgtg caaaaggagc aaagtcgggg cggagtcatc
      481 tttgcccggg atgaagcggt ggacgaagcc gaggtcgggg ctcatacgtt ctctccggcc
      541 gggacgcgca atggtgggag tacttcttcg agcctgtcgc ggcgggtttc gagccaggga
      601 gggagcttga ggtgcgtgcc gagttcttcg tctggttcgt ccacggtgaa gccgggggga
      661 tcggtagcta tctcgaacag gaccccgccg ggctcacgga agtagatgga tctgaagtag
      721 ttgcggtcga ggacgggcgt gacgttgtag cctagagcgg agatctcctc tcgcagcgcg
      781 agctggatct cgtcgtcggg gacgcggaaa gcgacgtggt gaactgtgcc gacgcccatg
      841 ctccccctgg gaaagcccgc cgcgcttacc aggtcaacca ggtttcccgg accgccctcg
      901 cctgatgaga gcctgatcct accttcagcc tcgtcgacct ggcgaaatcc tagagtctcg
      961 gtcatcaacc tcgcggtgct ctctgtggtc tcgaccgcga gggcgacgtg gtggaagccc
     1021 ctgaccgtgt gctcggctgg aacgatacta ccttcccatc ctgcgcgccc gtcttcggtg
     1081 gccacgagct ctatgcggag gcggtcaggg tcctggagag taagaaccgt atccccgaag
     1141 cggtcgcggg gtttctcgaa gcgtacgcct ttctcgacca ggcgctccgt ccagtagccc
     1201 aacgaggtag ccgggatgga gaacgaggta acgacgagct ggccggcccc gatccttccc
     1261 cccggcgcgt gcgcccaggg gaagaaggtc atgatcgtcc caggggtgcc agcgctgtct
     1321 ccgtagtaga ggtggtaggt ctccgggtcg tcgaagttga cggtcttctt tacaagccgc
     1381 attcccagga tgccggcgta gaagtccacg ttgcgctgcg gttcgcccga gatggctgtt
     1441 acgtggtgga ttcctgtagt ctgcggcgcc attgttctcc tctccgcggt atcatccgac
     1501 gccggataat cttagcaccg gcggacgcca gtccaacggt gccctcgctt atactggtct
     1561 ggttgcggac tttcctgaag gagggggctt tgcctctggc actcgagttc tgtgcggcgt
     1621 tggttgcgat cgtcttcgcg gctgtgctgt ttacgaacgc ggtcgagata ctgggtgggc
     1681 ggctcaacct cggggcaggg gctgtcggga gcgtgcttgc cgccgttggc acggcgctgc
     1741 ctgagaccat gatcccggtg gtagcgatac tcgccgccgc gttcgcggga gaaggtggag
     1801 aggccgcggg tgagataggg atcggggcca tcctcggcgc gccgttcctt ctgtccacgc
     1861 tcgctatgtt cgtcgtgggg tcctccgccc tcgcgtaccg cgggcggcgg gaacagggag
     1921 cgaagatccg gtgcagcgag gccagtgtgg gggacgagtt cccgtggtgc aagaccccgg
     1981 cgaaagaggt gatcgtggac gagaagacca ttgcccgcga tatggggttc ttcctgatct
     2041 tcttcgccgt ggccgcggga gccgggatcg tcgagcttcc cctcgcgctg cgcttcgccc
     2101 tggccgctat cctgatctcc gcctacgccc tttacgtacg gcggactcta cattctggcg
     2161 gcgcgttgga agaggtgccg cagagcctca cgctctggcc gttcgcttcg cggcctccga
     2221 cgtggatcgt cgtctgtcag gcggtccttt ccctggcgct catcgtcgtc ggcgccgagt
     2281 ttttcgttgg cgccgtcgag ggcgccgcgg aggtcgtggc gctaccggcg ggtctggtcg
     2341 ccctcatact cgcccccctg gcgacggaac tgcccgagaa gataaactcc gtcctctggg
     2401 tgcgcgaagg caaggacacc ctggcactcg gcaacatcac aggcgccatg gtcttccaga
     2461 gcaccgtacc tgtcactttg ggcgtcctgt tcacgccgtg
//
LOCUS       DATAIR010000046         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_139608, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000046 DATAIR010000000
VERSION     DATAIR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139608"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..2329
                     /locus_tag="VHM69_01230"
     CDS             29..2329
                     /locus_tag="VHM69_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX2739039.1"
                     /translation="MATGKRAPRKSTKKGSSRKGPSAKTTRKGTSPLTRVRKGLAEAL
                     TREAVGIALLAIGLFFSAAFASGRGAFLGDAGSLVTTHMLGLAGLGLAPLAAIAGLLL
                     LLGRLPGRVVAGAALLLLACAATLATTLPQRQMFSAGYYPEAGGILGSGFYSGVQWAG
                     GAIGAALAIGLLYALGLSLLTGVSFGAALGGIKSVVEKCLGWSRMFLARLRERREEPP
                     DPAPKRASKSRDRGSARVREVRHEPNSEAPAGAPEPFPPREFEVVLPERRPAQAFEGE
                     QELVPGEYTPPPFSMLGLGHGDPEHDAEGTSQRLTRALSDLGVEAHVVRAVVGPRVTR
                     YELRLGSGVKVGKVRNLQQDIAYALAATEVRILAPIPGKSAVGVEVPNTRPAKVTLGD
                     VFQSYPDANDWTLPVGLGKDISGRAVFFDLSEMPHLLVAGTTGSGKSVMLNGLLTSLL
                     LTTDPRQVKMVLIDPKRVELSQFGGVPHLITPVVTDVKKAANALAWAVAEMERRYEVL
                     EKLGVRSPEGYNDRAETPMPYVVVVIDELADLMMQAGAKVEDAIIRLAQKARAVGIHL
                     VVATQRPSVDVITGMIKANVPSRIAFAVSSQVDSRVILDTPGAEALLGMGDMLYKPVS
                     AARPSRVQGAFISEAEVDRVVSATVEAASGRVEARFVEEVTEPRNVKEESDEPEDDLL
                     PEAANFVVSTQQASVSAVQRRFRVGYSRAGRIIDALERKGVVGPYEGSKSRSVVATTM
                     DREALFGDGEGSAARTDGKADLAEGW"
BASE COUNT          402 a          745 c          831 g          392 t
ORIGIN      
        1 ctacaggctt atgggctaga cttaaggcgt ggctacgggc aagcgcgcgc caaggaagtc
       61 tacgaagaag ggctcttcgc ggaagggtcc ctcggccaag actacccgca agggaacgtc
      121 gccgctgacg cgagtacgca aaggcctcgc cgaggctctt acgagggagg cggtcgggat
      181 cgcgcttctc gcaatcggcc tcttcttcag cgccgcgttc gcgagcggac gcggggcatt
      241 cctcggggac gcaggctctc tcgtgaccac gcacatgctc gggctcgccg gcctcgggct
      301 cgccccgctc gcggcaatcg ccgggctgct tcttttgctt ggccgcttgc ctggcagggt
      361 cgtcgccggc gccgcgctct tactactcgc atgcgcggcc accctggcaa ccacattacc
      421 gcagagacag atgttctccg ccggatacta tcccgaggcc gggggcattc tgggcagcgg
      481 attctattca ggggtgcagt gggccggggg cgccatcggg gcggcgctcg ccatcggtct
      541 cctctacgcc ctcggcctgt ctttgctcac gggagtgagc ttcggcgcgg ctctgggtgg
      601 gataaagtcc gtagtcgaga agtgtctggg ctggagcagg atgtttttgg cccggctgcg
      661 cgagcggcga gaggaaccgc cggatccagc cccgaaacgc gcatcgaagt cgcgtgatag
      721 aggctctgcg cgcgtccgtg aggtgcgcca tgagccgaac tcggaggctc cggcaggcgc
      781 ccctgagcca ttcccgccca gggagttcga ggtcgtcctg ccggagcgca ggccagccca
      841 ggcttttgaa ggtgagcagg aactggtccc tggcgagtac acgcctccgc cgttctccat
      901 gttggggttg gggcatgggg atcccgagca cgacgccgag gggacaagcc agaggctcac
      961 ccgggcgctc tccgacctag gcgtcgaggc gcacgtggtg cgggccgtcg tcgggccgcg
     1021 ggttacccgc tacgagctgc gactcgggag cggcgtgaag gtcgggaaag tcaggaattt
     1081 gcagcaggac atagcctatg ctctcgccgc gaccgaggtc aggatcctcg ccccgatccc
     1141 cggcaagagc gccgtcggcg tcgaggtgcc caataccagg cctgcgaaag tgacgctcgg
     1201 ggatgttttc cagtcctacc ccgacgccaa cgactggacg cttccggtgg ggctcggcaa
     1261 ggatatctcg ggaagggcgg tcttcttcga cctctccgag atgcctcacc tcctggtcgc
     1321 ggggacgacc ggcagcggga agagcgtcat gctcaacggg ctattgacct cgctgttgct
     1381 caccaccgac ccgcggcagg tcaagatggt cctcatcgac cccaagaggg tggagctctc
     1441 gcaattcggc ggggtgccgc acctcatcac gccggtcgta accgatgtca agaaggcggc
     1501 caacgcgctg gcctgggccg tcgccgagat ggagcgccgc tacgaagtgc tcgagaagct
     1561 cggggtgagg tcccccgaag gatacaacga cagggccgag accccgatgc cctacgtagt
     1621 cgtcgtcata gacgagctcg ccgacctcat gatgcaggcc ggggcgaagg tcgaggatgc
     1681 gatcatcagg ctcgcccaga aagcgcgggc tgtgggcatc cacctcgtag tcgccaccca
     1741 gcggcccagc gtcgacgtca taacgggcat gatcaaggcc aacgtcccaa gccgcatcgc
     1801 cttcgcagtc tccagccagg tggacagcag ggtgatcctg gacacccctg gggcggaggc
     1861 cctgctcggc atgggagaca tgctctacaa acccgtctcc gcggcgaggc cgtcgcgcgt
     1921 gcagggcgct ttcatctcgg aggccgaggt ggaccgtgtc gtctcggcca ccgtcgaggc
     1981 ggcttcgggg cgcgtggagg cccgcttcgt cgaggaagtt accgagccga ggaacgtcaa
     2041 ggaggagagc gacgagccgg aggacgatct cctgccagag gcggccaact tcgtcgtctc
     2101 cacgcagcag gcgtcggtga gcgcggtgca gcggcgattc agggtcgggt actcacgggc
     2161 tggcaggatc atcgacgcgc tggagcgaaa gggcgtcgtt ggcccgtacg agggttccaa
     2221 gagccgctcc gtggtggcga ctacgatgga tcgcgaggcc ctgttcggcg atggtgaagg
     2281 ctccgcagcc cgcactgacg ggaaggcaga tctggccgag ggttggtagc ttttgtgata
     2341 ggcttctagg cgccatggac gactggcagc
//
LOCUS       DATAIR010000047         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_279499, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000047 DATAIR010000000
VERSION     DATAIR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279499"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..615)
                     /locus_tag="VHM69_01235"
     CDS             complement(64..615)
                     /locus_tag="VHM69_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2739040.1"
                     /translation="MREPNEVRRLEDRIFTVPNALSLARLFMVPIVVALLLNRADGLA
                     AALFVLAAVTDFLDGRLARRAGPTRLGQILDPVADRLMLSSVAVVLAMRGLLPAWAVA
                     ILVGRDLLALAGSIVVGSKIRVNWVGKVATAMLMGAIALVVLVPGTVIGEIMFYAGFG
                     LSIVAGLMYLRSVRRLLGGGEVR"
     gene            complement(727..1260)
                     /locus_tag="VHM69_01240"
     CDS             complement(727..1260)
                     /locus_tag="VHM69_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2739041.1"
                     /translation="MANLKPGEKAVPFELPGVDDTRHALEDYADKDVVAVVFTCNHCP
                     YARAWEERLIGIQADYAARGLQLVAISANDAKTYPADSFPGMKQRSEEKGFNFPYLYD
                     ESQEVARAYGAERTPEIFLFDNGGTLRYHGAVDDNYDDPAAVRSHYFIDAIEAILDDR
                     EPATTETAPVGCTIKWK"
     gene            complement(1344..1661)
                     /locus_tag="VHM69_01245"
     CDS             complement(1344..1661)
                     /locus_tag="VHM69_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739042.1"
                     /translation="MSFLYYEECPSHELALGRLREVMAEEGIPSEVEVVKVESEDQAR
                     QLRFVGSPTIRVDGLDIDPPSDSLYALTCRAYSLEGDRISPLPSREMIRRAFRPPAKS
                     RPG"
     regulatory      2036..2114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2226..3176
                     /locus_tag="VHM69_01250"
     CDS             2226..3176
                     /locus_tag="VHM69_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739043.1"
                     /translation="MYLVVGALAIGAYFFIPSPVAQESLRPLSSLAALGAFVAGILVH
                     RPRRPLPWYLFTLGSLLSILGIVTYVYYEITLGKTPFPSLADVFFLAGYPCVAAALLI
                     MQSRRLARDRASVVDPIIVSVGVGMLAWVFLMRPYLDDLSLSLPQRLVSVAYPLMDVL
                     LLAVLVRMLLVSGRRPFAYYPLVAGVLCTLAFDASFTVSSLAGTYETGGLVDAFEMLF
                     LLLLGTAALHPSMVDLSDGIVHDPETKLTRRRLALLAAASLMAPGLLALQAARGEPLN
                     VPVIVGGSVVLFSLVIVRMMGLVRNIEQAAAEIQHLNEGL"
     gene            3225..>4270
                     /locus_tag="VHM69_01255"
     CDS             3225..>4270
                     /locus_tag="VHM69_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2739044.1"
                     /translation="MTTARNEAESANRAKSEFLANMSHELRTPMNGVVGMTGLLLDTA
                     LSAEQREYAETIRTSGESLITVINDILDFSKIEAGKVDFEALDFDLGTTVDETLGLHA
                     ALARTKGLELASLVESEVPTALRGDPGRLMQVLTNLLGNALKFTEDGEVVLRVKLAEE
                     TSHTAAVRFEIKDTGIGITEEQQERLFLAFTQAESSTTRRYGGTGLGLVICKQLVEMM
                     GGQIDVESEPGKGSTFWFTVPLEKSPSRGHYAPARHADLGGLRILVVDDNETNRKILH
                     EQVISWGMRNGQAEGGSSALKMLRRASHEGDPYELAILDLHMPEMDGIELAHRIKKDP
                     QISKTKLILLTSMGL"
BASE COUNT          841 a         1299 c         1275 g          855 t
ORIGIN      
        1 cttcagttgc ttgctggaca gggactcagg cgtgtcccct gtgtgctgcc atgaagatcg
       61 ccatcatcga acctcgcctc cgcccaggag gcgccgtaca ctcctcaggt acatgagccc
      121 cgccacaata gagagcccga aaccggcgta gaacatgatc tctcctatca ccgtgccagg
      181 tactagcacg acgagcgcga tcgcccccat cagcatggcc gtcgccacct tgcctaccca
      241 atttacccga atcttgctcc caaccacgat gctcccggcg agcgccagga gatcacgccc
      301 gaccagtata gcgacggccc aggcaggcaa gagtcctcgc atggccagca ccaccgcaac
      361 gctggagagc atcagccggt ccgccaccgg gtcaaggatc tgaccgagcc tggtcggccc
      421 ggctcgccgc gcgagcctgc cgtccaggaa gtcagtcacg gccgcgagca cgaataaagc
      481 ggccgccaac ccgtccgccc ggttgagcag caacgccacc acgatcggta ccatgaagag
      541 ccgcgcgagt gagagggcgt tgggcaccgt gaaaatgcgg tcttcgagcc gtcttacttc
      601 gttgggctcc ctcattgttc tatcggctgc tcttaacctt gatcctcacg tccacgccgc
      661 acagatcata cgctaccaca acgcgacttc aggtggcgtg tgaagaaaac gtatcctact
      721 cggaagtcac ttccacttta tggtgcaccc tactggagcg gtctccgtgg tggccggctc
      781 gcgatcgtcg aggatcgcct cgatggcgtc tatgaagtag tggctcctca cagccgccgg
      841 atcgtcgtaa ttgtcgtcta ccgcgccgtg gtagcgcaaa gtgccgccat tgtcgaagag
      901 aaagatctca ggtgtgcgtt cggctccgta ggctcgggcg acctcctggg actcatcgta
      961 gaggtagggg aaattgaatc ccttctcctc agagcgctgc ttcatgccag ggaagctatc
     1021 ggcggggtac gtctttgcgt cgttggcgct gatggcgacg agttgcaagc cgcgagctgc
     1081 gtagtcagct tgtatcccga tcaaacgctc ctcccaggct cgggcgtagg ggcaatggtt
     1141 gcaggtgaag acgaccgcta ctacgtcttt gtccgcgtag tcctcgaggg cgtgtcgcgt
     1201 atcgtccacg ccgggcaact cgaagggcac tgccttttca ccaggcttta ggttggccat
     1261 gtcacctctc cttttccgca agatttcgcc gcacgggtaa tgatccctgg aaacgtttta
     1321 ccaccgcccg acccggtcga cggtcagccg ggtcggctct tcgccggggg cctgaaagca
     1381 cgccggatca tttccctgga tggcagcggg gagatgcggt cgccttcgag gctgtaggcg
     1441 cggcaggtca gggcatatag agagtcgctc ggtgggtcaa tgtctagccc gtcgacgcgg
     1501 atggtcgggg aaccgacgaa gcgcaactgg cgggcctggt cttcggactc gaccttgaca
     1561 acctccactt cgcttgggat gccttcctcg gccatgacct cgcgtaggcg cccgagcgca
     1621 agttcgtgag acggacactc ttcgtaataa aggaagctca ctcgcacgag ttacctcaga
     1681 tctgttcgtt acgtacccca gaagctcctg cacgaacctg ggattcacac cttcagatac
     1741 ggtgctaaac cttctttgcc gtgccaagcg ctttctcgat cattctaatg ttatcgtttt
     1801 ctggctcttc taccaaagtg aatcgcgata gctccgccaa gagcgcgccg atctgaaacc
     1861 atcgagagca caatacggtg caacactgga cagggcagag gagagaaacc agcatttacg
     1921 aacggggcga cattgaccgt agtaagaggc aggagagata tgacagccac tgcgtgtcta
     1981 gtaaatatgc aatgctttag ctacacttcc tccagtcgcg cgcatcttct gagcattgcg
     2041 ataaaggcaa ccccgccgtg agacgggtgc gcaaagccgc gggcctctca agtggaggca
     2101 gccgggccgc cgaagggaga ccgccagtga accgattacg ttcttcccac accgggacaa
     2161 atcaaaccta tcccgaacct gctagaggaa agagggggtt cgactggcgg ctgcgagcgt
     2221 ggcttgtgta tctggttgtg ggcgccctcg caataggcgc ctacttcttt atcccctcac
     2281 cagtcgcgca ggaaagcctc cgcccccttt ccagcctggc cgcgctgggg gcgttcgtgg
     2341 ccggcatcct ggtgcatcga ccgaggcgtc cgctgccatg gtacctgttc acgctcggtt
     2401 cactactctc catcctcggc atcgtgactt acgtctacta cgagatcacc ctgggaaaga
     2461 ccccgttccc gtctttggcc gacgtgttct tcctcgccgg ctacccgtgt gtggccgccg
     2521 cgctgctgat aatgcagagc cggaggctcg cacgagaccg ggcaagcgta gtagacccga
     2581 tcatcgtctc agtgggggtt gggatgctcg cgtgggtctt cttgatgcgc ccttaccttg
     2641 atgacttgtc gctctcccta ccgcagcggc tggtctccgt agcctacccg ctgatggacg
     2701 tgcttctgct agcggtgttg gtgcggatgt tgctcgtgtc cggcaggcgc ccgttcgcct
     2761 attatccact tgtggcgggt gtgttgtgca cgctggcctt cgacgcttcg ttcaccgtat
     2821 cctcccttgc gggtacctac gagaccggag gcctggtcga cgccttcgag atgctcttct
     2881 tgctgttgtt ggggaccgcc gcgctgcacc cttcgatggt ggacctctca gatggaatcg
     2941 tgcacgatcc cgagacgaag ctcacgcgga ggcgtctcgc gttgctcgcc gcggcctccc
     3001 tgatggcgcc cggtttgctc gcgctgcagg cggcgcgtgg cgaacccttg aacgtgcccg
     3061 tgatcgtcgg aggctcggtg gtgcttttct cgctcgtgat cgtccgcatg atgggcctcg
     3121 tgcgcaatat cgagcaagcc gcggcggaga tccagcacct caacgaggga ctgtaagcgc
     3181 gggtcgagga gcgtacttcg caactcgaag ccgccatcga cgagttgacg acggccagga
     3241 acgaggccga gtcggctaac cgggcaaaga gcgagttttt ggcgaacatg agccacgagc
     3301 tacggacgcc catgaacggt gtcgtcggca tgaccggtct cttactcgat acggcgctga
     3361 gcgcggaaca gcgcgagtat gccgagacga tacgtacctc cggagagagc ctgatcacgg
     3421 ttatcaatga tatcctcgac ttctccaaga tagaggccgg aaaggtggac ttcgaggcac
     3481 tcgacttcga cctgggtacc acggtcgatg agacccttgg attacacgcg gcgctggccc
     3541 gcaccaaggg tctggagttg gcgagtcttg tggagagcga ggtgccgaca gcgcttagag
     3601 gcgaccccgg tcgcctgatg caggtcttga ccaacctcct cggcaacgcg ctcaagttca
     3661 cagaggatgg ggaggtagtc cttcgcgtca agctggccga ggagacctcc cacacagcgg
     3721 cggtgcgctt cgagataaag gacaccggca tagggataac cgaagaacag caggaacgcc
     3781 tctttctggc ctttacccag gccgaatcct caaccacccg ccgctacgga ggcacggggt
     3841 tgggacttgt catctgcaag caactggtgg agatgatggg agggcagata gatgttgaga
     3901 gcgagcctgg caaggggagc accttctggt tcaccgtgcc actggagaag tcaccaagca
     3961 gaggccacta tgctccggcc cggcacgctg atctgggtgg ccttaggatc ctggtcgttg
     4021 acgacaacga gaccaaccgc aagatactgc acgagcaggt catctcatgg gggatgagaa
     4081 acggtcaggc cgagggcgga tcaagtgccc tcaagatgct taggagagct tcccatgagg
     4141 gtgatcccta cgagcttgcg atcctcgacc tgcacatgcc tgagatggac ggcatagagc
     4201 ttgcccatag gataaagaaa gaccctcaga tctctaaaac caaactcata ctccttacct
     4261 cgatgggact
//
LOCUS       DATAIR010000048         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94818, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000048 DATAIR010000000
VERSION     DATAIR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94818"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VHM69_01260"
     CDS             complement(<1..343)
                     /locus_tag="VHM69_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739045.1"
                     /translation="MAKETARIAAVAAALGFLVMAAFQAALALGAPLGSAAWGGAHEG
                     QLPMGLRIASAVAVAVYSLFALLVLGRGGFQGVPLPYGVLRWGTWVLVVLMILGMFMN
                     VASSSGWERFLW"
     gene            complement(432..1100)
                     /locus_tag="VHM69_01265"
     CDS             complement(432..1100)
                     /locus_tag="VHM69_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739046.1"
                     /translation="MNNATRATVSAFGVLAGLAGIEHGVGEVLQGKTAPSGVVISSWP
                     GSEVFGILAGEPAMTLVPNLLATGILAVLAVLVSVTFLLWVTVFVGTKHGGLGLILLS
                     LALLLVGGGFGPPVLGFIIGLAAARMNTPLDRRRTYLSDGPRRFLAGLWPWSFAAAVI
                     AWLSLLPGTVLLDQFVGVSNTNLVVAVLTLSAFGLLLLRLFAGLARDAQRQKGSHRAH
                     PVSV"
     gene            complement(1285..2136)
                     /locus_tag="VHM69_01270"
     CDS             complement(1285..2136)
                     /locus_tag="VHM69_01270"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX2739047.1"
                     /translation="MKKSRDMRITTRVALGLAGSAALLAGAGWLGLRVRREPFVPHSE
                     KTPELDLAELPDGLPEPARRYFRVVFGEKVPRIETAVVWGRAGFKIMGVWTPMRFKGY
                     YAEGRGFRRDMEITWFGAPVLRGTDAYLDGKGSLEITGLLNISGRGENFDQGENLAMW
                     GEAPFTTPSAPVLDPRIRWKPIDATSARLIVPFREAEESLRVEFDQHTGLMKSVSGMR
                     YRNQEETKTSWRGEFSDWRTIHGIKVPHRAVAAWEDEREPYGIFEIEGAEYNVDVSEK
                     VPGTGPR"
     gene            2483..2971
                     /locus_tag="VHM69_01275"
     CDS             2483..2971
                     /locus_tag="VHM69_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739048.1"
                     /translation="MPVLEEVEVIAMTTTTTKRAPPRMFLRKVGHFLWHYLQMCMACC
                     LGGVTLGFAFFGGAALIGYPDLILKAPFFSTLVLAIVLTVPMVAWMRFRHHEWRPTLE
                     MGSATMGLGIVLIALGAIGLVPVSGMFEWVASLACPVMLIPMLLRLNLYTGAMDHHAH
                     AS"
BASE COUNT          618 a         1048 c          938 g          608 t
ORIGIN      
        1 cccataagaa gcgctcccat ccgctcgacg acgctacgtt cataaacatt cccagaatca
       61 tcaggacgac gagcacccat gttccccatc ggaggacacc atacggaagt ggcacaccct
      121 gaaaacctcc acgccccagc acgagcagcg cgaagagcga gtagaccgct accgcaacgg
      181 cgctggcgat ccgcaggccc atcgggagtt gcccctcgtg ggctccaccc cacgcagcac
      241 tccccagtgg ggcccccagc gccaacgcag cctggaaggc ggccatcacc aggaagccca
      301 acgcagctgc cacagcggca atccgggccg tttccttagc catctttggc tcacctccgc
      361 gcttggctcg tggatggatg actttcgttg gctgtttgca tcgcaaggct cctttcgggt
      421 tccacttcct ttcacacgct caccggatgt gcccgatgcg agcccttctg cctctgagcg
      481 tcgcgggcga gcccggcgaa cagcctcaac agcagcaacc cgaaggcgga gagcgtgagg
      541 acggctacga cgaggttcgt gttgctcact ccgacgaact gatccaggag gaccgtgcca
      601 ggcaacagcg agagccaggc gatcacggcg gcagcgaaag accacggcca cagccccgcc
      661 aggaaccggc gagggccgtc ggagaggtaa gtgcgccgtc ggtcgagcgg ggtgttcatc
      721 ctggccgcgg ctaggccgat aatgaagcca aggaccgggg gtccgaagcc gccccccacc
      781 aggagcaggg cgagggagag caggatcagg cccaagccgc cgtgctttgt tccgacaaac
      841 accgtcaccc acaaaagaaa ggttacggag acgaggacgg cgaggacggc gaggatgccg
      901 gtcgccagga ggttgggaac gagggtcatg gctggctccc cggccaggat accgaacacc
      961 tccgagccgg gccaggacga gatcacgact ccactgggag ccgttttgcc ctgaagcacc
     1021 tcgccgacac catgctcgat gcccgccagc ccggcaagga cgccgaacgc cgagaccgtc
     1081 gccctggtcg cgttattcac tcgactcctc cttcccggtg ctggaggcat ggccaaccgt
     1141 cggttggtag aacgcaactt cgcgtcctgc ccgctctaaa tcctgttccc accctactcc
     1201 cgaagcactg ccccgccctg acacagattg agcagtttcc tggtcaaatc gcgccatttg
     1261 gtttgcgggg tggcatcgca gactctatcg tggtccagtc cctggaacct tctcggagac
     1321 atccacgttg tactcggcgc cttcgatctc gaaaatgccg tagggctccc gctcgtcctc
     1381 ccaggctgct acagcccggt ggggaacctt tatcccgtgg atcgtcctcc agtccgaaaa
     1441 ctcgccgcgc caagaggtct tcgtctcttc ctggttccgg tatctcatgc cggacacgct
     1501 cttcatcaag ccggtgtgtt ggtcgaactc gacccgcaag gactcttccg cctctctgaa
     1561 gggaacgatc aggcgcgcag acgtggcgtc tatcggcttc cagcggatgc gaggatcgag
     1621 gaccggcgcg gaaggggtgg tgaacggcgc ttctccccac atcgcgaggt tctcgccctg
     1681 gtcgaagttc tctcccctgc cggatatgtt cagtagaccg gtgatctcga ggcttccctt
     1741 tccgtccagg taggcgtcgg tgccccgcag gaccggcgcc ccgaaccagg tgatctccat
     1801 atccctgcgg aagcctcgcc cctcggcgta ataacccttg aagcgcatgg gggtccacac
     1861 tcccatgatc ttgaagccgg cccttcccca cacgacggcg gtctcgatcc tgggaacctt
     1921 ctcgccgaag accacccgga aatacctgcg tgccggctcg ggcaggccgt caggaagttc
     1981 ggccagatcc aactcagggg tcttctccga gtgcgggacg aagggctcgc gcctgaccct
     2041 caaccccagc caaccggccc ccgccagcag ggcggcggag cccgccaatc ccagagccac
     2101 cctggttgtg attcgcatat ctctgctttt cttcattcct acactctcct taccggagcg
     2161 aagtccgtct gacgccagaa agcggcgacg ccgagacggt ggatcaatcc gtgcaggatc
     2221 agcatagtcc cgagaatgac gcgctcgatc atggcttcct ccttgtttgt acggcgctgt
     2281 ttgccgatca aaccgtatcg gtcggcgatc tctccgcttg agccagatgg cccattcacc
     2341 tggcaaatat tgaccaactc gtactctaag agcgagttgg tcaatattgc ccacgacaat
     2401 gacccgtttg ggtcatgtgc cacccggata cagagcgtac ggtgagcttg tcacaaaagg
     2461 gtggactggc tgcggtgctt ccgtgccggt ccttgaggaa gtggaggtga tcgccatgac
     2521 cacgactact accaaacgtg caccaccaag gatgttcctt cgcaaggtgg gacacttcct
     2581 gtggcactac ctgcagatgt gcatggcctg ttgcctcggg ggggtgacct tgggcttcgc
     2641 gttcttcggc ggagcggcgc tcatcggcta tcctgacctg atcctcaaag ctcccttctt
     2701 ctccaccctg gtactcgcca tcgttctcac ggtgccgatg gtcgcatgga tgcgctttcg
     2761 ccaccacgaa tggcgcccca cgcttgagat ggggtcagcg acgatggggt tggggatcgt
     2821 cctgatcgct ttgggagcga tcgggctcgt cccagtgagc gggatgtttg agtgggtcgc
     2881 aagcctcgcg tgcccggtga tgctcatccc catgctgttg cgtctcaacc tctacacggg
     2941 agcaatggac caccacgcgc acgcctcatg agccctggtt gcgagcgcta ggtcgctatg
     3001 cgagagctag atccccaagt gaacgacgca ctgccgctcc agaggctcca ggatccttct
     3061 tgtctggagc ctccggactc ttctcccgca cttgtttgtc taacgttgta cggagaatct
     3121 gtctgacaat agtgggttga aaaggaggat aagagagagc ctatgccagc agaggagagt
     3181 aacaaggcca tcatgcgccg attttgggag gt
//
LOCUS       DATAIR010000049         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_94833, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000049 DATAIR010000000
VERSION     DATAIR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94833"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="rapZ"
                     /locus_tag="VHM69_01280"
     CDS             <1..786
                     /gene="rapZ"
                     /locus_tag="VHM69_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2739049.1"
                     /translation="YFCIDNLPPRMIPAVMETTAQGKGGPDGVVVVMDMRGRRYFGEE
                     LERNLEVVEREEGWEHRILFIDSDDPTLVRRYKESRRPHPAARDGDVLDAIRRERRDL
                     ANVREMADLVVDTSGYSALDARLRFKKLAESFSGRMTVSLISFGFKHGTPLDVDTLLD
                     VRFLPNPHYDPELRPLTGHDAPVREAVLGSKDCQEFLKRTGGLLSFLIPRYAAEGKTY
                     FTLGVGCTGGRHRSVAITEELAKRLRQEDTGIDLFIRHRDVEK"
     gene            956..>1681
                     /locus_tag="VHM69_01285"
     CDS             956..>1681
                     /locus_tag="VHM69_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX2739050.1"
                     /translation="MAFGGGTGLPVLLRGLRDRVGDLTAVVTVADDGGSSGRLRQELG
                     VAPPGDVRNCLVALAGRRRLAEVFNYRFEAPGDLNDHSVGNIIIAALSDMAGGFCEGV
                     EQAARFLRIKGRVFPAATESLTLVVRYADGTVTRGESVVHEVGKQVSEVAVEPSGVPA
                     PQGVIEAIEDADVLVLGPGSLFTSTIPALLGVGVREAMADFGGPVLYVANVMTQQGET
                     GDFTVSDHVRAITEHVGPVVTDVL"
BASE COUNT          309 a          495 c          561 g          316 t
ORIGIN      
        1 tacttctgca tcgacaacct tccgccgcgt atgatccccg ccgtcatgga gacgaccgcg
       61 cagggcaaag gtggtccgga cggtgtggtc gttgtgatgg acatgcgtgg gcggcggtac
      121 ttcggcgagg agcttgagcg caacctcgag gtcgtcgaga gggaggaggg ctgggagcat
      181 cgcatcctgt tcatcgattc ggacgacccc acgctcgtca ggcgctacaa ggagagccgc
      241 aggccccatc ccgccgcccg cgacggcgac gtcctcgatg ccataaggcg cgagaggcgg
      301 gatcttgcga acgtgcggga gatggccgac ctcgtcgttg atacttctgg atattccgcg
      361 ctggacgcgc gcctgcgttt caagaagctc gccgagtcgt tctccggcag gatgacggtc
      421 agcctgatct ccttcggctt caagcatggt acccccctcg acgtggacac gcttctcgac
      481 gtgcgtttcc tgcccaaccc tcactacgac cccgagctca ggccgctcac gggccacgac
      541 gcgcccgtgc gcgaagccgt gctcgggtcc aaagactgcc aggaattcct caagagaacc
      601 ggcggccttc tctctttcct cattccccga tacgccgcag aaggcaaaac ctacttcacg
      661 ctcggcgtcg gttgtaccgg cggaagacac cgctctgttg ctataaccga ggaactggcc
      721 aaacgcctaa gacaggaaga tacaggaata gatctcttta tccggcacag ggatgtggag
      781 aagtaggttt caggcttcag gaagattggt ttcatgctac gctggtatag cgttgcgtga
      841 ttctaacggg ggagccggta ggatcatcct tcgatagcaa ccccatgtaa gcaaactgaa
      901 acctgcgatc cggatatact ccgtctgcgt gaatagggtc gagaatttaa gggttgtggc
      961 gttcggcggc ggtactgggc tacctgtgct cctcaggggt ctgagggaca gggtcgggga
     1021 cctgacggcg gtagtcacgg tcgccgacga tggggggtcc agcgggcgat tgcgccagga
     1081 gctcggggtc gcgcctcctg gcgacgtgag gaattgcctg gtggcgctcg cggggcgccg
     1141 caggctcgcc gaggtcttca actaccggtt cgaggctccg ggagatttga acgaccacag
     1201 tgttggcaac atcatcattg cggccctctc cgacatggcc ggcgggttct gcgagggcgt
     1261 ggagcaggcg gcgcgttttc tgaggatcaa aggccgagtc ttccccgcgg cgacggagag
     1321 cctcacacta gtcgtccgct acgccgacgg gaccgtaacg cgcggagaat ctgtggtcca
     1381 cgaggtcgga aagcaggtct cggaggtagc ggtcgagccg tcaggggttc ctgcgccgca
     1441 gggggtcatc gaggccatag aggacgccga cgtgctggta ctcggtcccg gaagcctgtt
     1501 cacgagcacg ataccggctc tactcggcgt cggggtacgg gaggccatgg cggatttcgg
     1561 cggacccgtc ctctacgttg cgaacgtgat gacgcagcag ggcgagacgg gcgactttac
     1621 ggtctctgac cacgtgcggg cgatcacgga gcacgtcggg cccgtcgtta ccgacgtgct
     1681 c
//
LOCUS       DATAIR010000050         9461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_371652, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000050 DATAIR010000000
VERSION     DATAIR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371652"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..2103
                     /locus_tag="VHM69_01290"
     CDS             79..2103
                     /locus_tag="VHM69_01290"
                     /inference="COORDINATES: protein motif:HMM:NF027792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HEX2739051.1"
                     /translation="MRDLNAPDGKESTAQNIEGVHLIFKTHLDVGFTDLAANVMEEYF
                     ESFIPRAIGAARELRKRERSERLVWTTGSWLIYEYLEGAAPAERRSLEEAIEAGDVVW
                     HGLPFTTHSELVDPGLFEYGLGLSQELDARFGKKTIAAKMTDVPGHTRAIVPMLADAG
                     IRFLHIGVNPGSTPPAVPPVFRWRDPDGSEVTVMYQRGGYGDLVTVPGMTEALAFAHT
                     GDNRGPQSAEQVIEAFEEMGTRVSGARIVASTLDIFARAMLAQRPDLPVVTGEIGDTW
                     IHGVASDPGKISRYRELCRLRRRWISESKDGDLSAFSRRLLMVPEHTWGLDEKTYLAD
                     YENYAADELNRVRGDASFVRQEASWIEQRAYVDEAIGLLDEEREREAEERLAAIAPQY
                     PNKAGFSQIDLSETHETAHFSIGFDPESGALNRLVDKATGRVWASEEHPIGLFRHQTF
                     THEDYSRFLDQYIIDWSKNQEWASEDYGKPGIERYAGEGGMWNLRMAGLYGREDDSGH
                     HFLVELEAPEERPEGYGCPGRVTVGVTLPYEERSVHLDVQWFDKPACRLPEALWLSFA
                     PEAPDVEGWTLEKMWRRISPLEVVPGGNRKLHAVGSRVEYADDRGGLLIDTLDAPLVA
                     PGEPSLLDFNDGQPPLLQGMHFNLYNNVWGTNFAMWYDDDARFRFVLRVI"
     gene            2134..3252
                     /locus_tag="VHM69_01295"
     CDS             2134..3252
                     /locus_tag="VHM69_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2739052.1"
                     /translation="MTGDSAVKVGVIGCGKISGIYLENGAIFDDVDIVACSDLVLERA
                     EAQAEAYGVPKACSPEELLADEEVEIVLNLTLPAVHAEVSLAALESGKHVYTEKPLAV
                     SREDGRRMLEVATERDLLVGCAPDTFLGGGLQTCRKIMDEGVIGESVAVTAMMMTHGP
                     EDWHPNPDFYYQPGAGPMFDMGPYYLTALTTLMGPVRRVTGSARVTFPERMITSQPHA
                     GTTITVNTPTHVAGIMDFEGGTVGTLVTTFDVWSEDQSRIDLYGTEGTLSLPDPNTFG
                     GPVRLWRSGEKAWTDVPLTHPYSKNSRGLGLADMAQALRSGRPHRASGELGMHVLDII
                     HAFLDSSENGEHVEVSSTFERPEALPATSPERIFGGGA"
     gene            3249..4004
                     /locus_tag="VHM69_01300"
     CDS             3249..4004
                     /locus_tag="VHM69_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2739053.1"
                     /translation="MRWDRISLQLYTVRELTSRDMPGTLRRISEIGYPAVEFAGYGGH
                     SPQDLRKVIDDLGMRASGAHVPLDSWETDPEMVLADMHTLGSTHAVVPIAPPERCGDE
                     ASVARLAGSFNRWGELCRQAGVTFSYHNHDFEFAPMDGTTMWDVLIRETDPDLVHLEL
                     DLYWVRYGGADPERILRDLGDRISLVHLKDMAPDEQRSDLPVGEGTMPWPELLQTAEE
                     AGVEWYVAEQDNPRDAIEDVRISLQHMRELASE"
     gene            complement(4046..4828)
                     /locus_tag="VHM69_01305"
     CDS             complement(4046..4828)
                     /locus_tag="VHM69_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX2739054.1"
                     /translation="MQNTYRVGLIVPSSNTTMETEIPRMILAREAERSGERFTFHSSR
                     MRMMNVTPEELRAMNAQMGRCAAELADARCDVVTTACLVAIMAQGAGFHRVAEDQIVD
                     ALHEEGAPAPVVSSAGALLKALEDLGARRVALVAPYMRPLTKLVSEYIESSGVMVQDA
                     LSLEVSDNLEVRRLDPENLKEHWKKVDLAGCDALVLSACVQMPSLSSIEPVEQACGLP
                     VISAATATTYCILQALELTTIVPGAGYLLSGQVNPVGVSSQS"
     gene            complement(5179..5949)
                     /locus_tag="VHM69_01310"
     CDS             complement(5179..5949)
                     /locus_tag="VHM69_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX2739055.1"
                     /translation="MLAEQRRGVILEELGRIGSVSVADLSKKLKVSDMTIRRDLEELS
                     GRRLLRKVHGGAIPVSRSAEPHFEQKRSLKRPEKLAIARAAQKFVQDGDTVAFSAGTT
                     TWHVASTLYPGHKDLTFVTNSTNVALTLQEHGWESIVLSGGMFRTPSDALVGPFADRT
                     LRTLNADVLFLGAHGVHPDAGLTTPNTAEAETNGCLVATAQKVVVVADSSKLGVVALA
                     RIIPLSRVDVFITDHKAPKQILREIELSGPKVVVAEPS"
     gene            complement(6065..6670)
                     /locus_tag="VHM69_01315"
     CDS             complement(6065..6670)
                     /locus_tag="VHM69_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEX2739056.1"
                     /translation="MNILDTRVYRWLEVATDFFLLNLMWLVACVPVVTIFPSTAAMFG
                     VVRDWVRGKEGGLARTFVTRFRENLGQSLLVGAVWTFFGVTLFLDFIVASQLSTGAEV
                     VLKSVLVLASSVYAFGSVFLFPVMVHYETGWRTVIKNSLLLSIGRLPTTLVCLVIVLF
                     MAGLTAFVPFLVIITGSITAYIVYRLCDREFRKIDAASGNR"
     gene            complement(6744..7487)
                     /locus_tag="VHM69_01320"
     CDS             complement(6744..7487)
                     /locus_tag="VHM69_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2739057.1"
                     /translation="MPEAYLSSVEEHYEILLARHYSWMRGDYVSKVRENLTFFEEAGV
                     APRSGGKALDLGCGPGFQSIALAELGFAVLSVDTSGMLLEELRSRAGCRDILAVLGDI
                     RDPGMYAAEGPFEVAVCMGDTLTHLATPREVEALVGDVRGALDQRGTLIFEFRDFTAE
                     LKGVDRIIPVRMEDDRIMATFLEYEADNVTVHDVIFEKGRHGWEARKSAYQKLRLGPD
                     EVARILRKAGFSILSRGEDGGFVRIVARA"
     gene            complement(7714..8823)
                     /locus_tag="VHM69_01325"
     CDS             complement(7714..8823)
                     /locus_tag="VHM69_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX2739058.1"
                     /translation="MVTSATRSKSSTASPEHYRVSVEEYTTFRKQGFLVVRGLLGPDE
                     VEELREHTELLMQGRLPEQVATMAGRDPGSDSGTSAQALEAPPEHLSPEEKAQYFLRV
                     HMLHRKLELHERYLLHPGVLDVLEALIGPDVLALQTMLFLKPPGKPGQGWHQDSYYIP
                     THPDTLCGAWFAIDDADELNGAMWMASGSQHEPVYPPKEGYGHGDERLGGIEHISGAS
                     DPDDDNNDLSRIAERYPQLLAPVEAGDVVFFGGHVLHRSKKNFTTDRFRRAFVGHYCN
                     ARSFTQWGANHDDDDPHTAPSVDPDTKMTNGSHILARGDTHLPFAKPAFGTPCAALLP
                     AEERRKESEYAAAMMADMDSGDMGAKPADPEAEHD"
     gene            complement(8944..>9461)
                     /locus_tag="VHM69_01330"
     CDS             complement(8944..>9461)
                     /locus_tag="VHM69_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2739059.1"
                     /translation="PLFRHLLTWADRGDPHLLAVTMAHMLASFVTGETAGRDVPQESL
                     PEAVERALTYLHASLEEDSSTSIHLSDLAGAACVSPEHLCRLFKRSTGRSPVETVRLA
                     RLDRAAVLLARSNYTVAEISTVCGFSSPFHFSRRFKDAFGLSPRDLRKRLATGATPPT
                     PRLLQRSPALR"
BASE COUNT         1799 a         2977 c         2980 g         1705 t
ORIGIN      
        1 agacttcgac gtgcccgagg cgcgggagat cgtcaaacgc ggcatgctct gggcgagcag
       61 gtaattaaca taatggaggt gagagacctg aacgcgccgg acggtaagga atcaacggca
      121 cagaacatcg aaggtgtgca cttgatcttc aagacccacc tcgacgtggg gttcacagac
      181 ctcgcggcca acgtgatgga ggagtacttc gagagtttca taccacgggc gatcggggcc
      241 gcgagggagt tgcgcaagag ggaaaggtcc gagcgcctgg tgtggacgac gggatcgtgg
      301 ttgatctacg agtacctcga aggggccgcc cccgccgagc gcaggtctct ggaagaagcc
      361 atcgaggccg gcgacgtggt ctggcacggg ctccctttca cgacccacag cgagctagtc
      421 gaccctgggt tgttcgagta cggtctcggg ctatcgcagg aactcgacgc caggttcggc
      481 aagaagacca tcgcagcgaa gatgaccgac gtccccggcc acacgcgggc aatcgtgcct
      541 atgctcgccg acgccgggat caggttcctc cacatcggcg tcaaccccgg ctcaacgccg
      601 cccgcagtcc ctcccgtctt ccgctggcgc gacccggatg gcagcgaggt caccgtcatg
      661 taccagaggg gcggctacgg cgacctcgtg accgtgcccg ggatgacgga ggcgctggcg
      721 ttcgcccata cgggcgacaa ccgcggtccg cagtcggcgg agcaagtcat cgaggctttc
      781 gaggagatgg ggaccagggt cagcggggcg aggatcgtcg cctcgacgct ggacatcttc
      841 gcccgagcca tgctcgcgca acggccggat ctgcccgtcg tgacaggtga gataggggac
      901 acctggatcc acggcgtcgc ctcagatccc ggcaagatta gccgctacag ggagctgtgc
      961 cgcctgcgca ggcgttggat ctccgagagc aaagacggtg atctctccgc cttcagccgc
     1021 cgccttctga tggtccccga acacacctgg ggcctggacg aaaagaccta cctcgccgac
     1081 tacgagaact acgcagctga cgaactgaac cgggtgcgcg gcgacgccag cttcgtacga
     1141 caggaggcgt cgtggatcga gcagcgcgcc tacgtggacg aggccatagg ccttcttgac
     1201 gaagaaaggg agcgcgaggc cgaggagagg ctcgccgcca tcgcacctca gtatcccaat
     1261 aaagcgggct tctcgcagat cgatctctcc gaaacgcacg agaccgcgca tttcagtatc
     1321 gggttcgacc ccgagagcgg cgcgttgaac cgtcttgtcg acaaggcgac gggacgcgtc
     1381 tgggcgtctg aggagcaccc cattggcctg tttcgccacc agacgttcac gcacgaggac
     1441 tactcgcgtt ttctcgacca gtacatcatc gactggagca agaaccagga gtgggcgtcg
     1501 gaggactacg ggaaacctgg catagagcgg tacgcaggtg aaggcgggat gtggaacctc
     1561 cggatggcgg gattgtacgg gcgtgaagac gactcggggc accacttcct cgtcgagctc
     1621 gaagcgcctg aagaacgtcc tgagggttac gggtgccctg ggagagtcac ggtaggggtc
     1681 acgctccctt acgaggagcg gtccgtccat cttgacgtgc agtggttcga caagccggcg
     1741 tgccggttgc cggaagcgct gtggctcagc ttcgcacccg aagcgcctga tgtcgagggc
     1801 tggaccctgg agaagatgtg gcgccgaatc tcaccgctcg aggtcgtgcc tggcggaaac
     1861 cgcaagctgc acgcggtcgg aagtcgggtg gaatatgctg acgaccgggg cggcctcctc
     1921 atcgacactc tcgacgcgcc gctcgttgcg ccgggagagc cttcgctgct cgacttcaac
     1981 gacggccagc cgccgctact gcaagggatg cacttcaacc tctacaacaa cgtctgggga
     2041 accaacttcg cgatgtggta cgacgatgac gcccgcttcc ggttcgtgtt gcgggtgatc
     2101 tagggagatg agccacgaga agatcggagg agaatgacag gggattctgc ggtaaaggtc
     2161 ggggtaatag ggtgtggcaa gatcagcggc atctacctcg aaaacggcgc gatcttcgac
     2221 gacgtcgata tcgtcgcctg ctcggacctg gtgctcgaac gcgccgaggc ccaggccgaa
     2281 gcctacggtg ttccgaaggc gtgttctcct gaagaactgc ttgctgatga agaggtcgag
     2341 atcgtcctta acctgaccct ccctgccgtc cacgccgagg tatcgctggc ggcgctcgaa
     2401 tccggcaaac acgtctacac ggagaagccg ctcgctgtga gccgagagga cggacggagg
     2461 atgctcgaag ttgcgaccga gagagacctc ctcgtcggat gcgcccccga cacattcctc
     2521 ggtgggggac tccagacctg ccgcaagatc atggacgagg gcgttatcgg ggagtccgtg
     2581 gcggtgacgg ccatgatgat gacccacggc cccgaggact ggcacccgaa ccccgacttc
     2641 tactaccagc ccggcgcggg cccgatgttc gacatggggc cttattacct gacggccctt
     2701 acgaccttga tgggacccgt gcgcagagtg acgggttccg cccgcgtcac gttccccgag
     2761 cgtatgatca cgagccagcc ccacgcgggc accaccatca ccgtgaacac gcccacccac
     2821 gtcgcaggaa taatggactt cgagggcggc accgtcggga cgctggtcac gaccttcgac
     2881 gtctggtccg aagaccaatc aaggatcgac ctctacggca cggaaggcac gctgagcctc
     2941 cccgacccga acactttcgg cggtcccgtg cgcctctggc gctcggggga gaaagcctgg
     3001 acggacgtgc ccctgaccca tccctattcg aagaacagca gagggctcgg gctcgccgat
     3061 atggcccagg ccctgcgctc gggaagaccc cacagggcga gcggcgagct tggcatgcac
     3121 gtgctcgaca tcatccacgc cttcctcgat tcttcggaaa acggcgagca cgtcgaggtg
     3181 agcagcacct tcgagcgtcc cgaggccctg ccggccacgt ctcccgagcg catcttcggg
     3241 ggtggggcat gaggtgggat cggatctcac tccagctcta cacggtccgg gaacttacct
     3301 cgcgtgacat gccaggcact ttgcgtcgga tctccgagat cggatacccc gccgtcgagt
     3361 tcgcaggcta cggcggccac tccccccaag acctgagaaa ggtcatcgac gacctcggca
     3421 tgcgcgcctc tggtgcacac gtcccactcg actcctggga gaccgatccc gagatggtcc
     3481 tcgccgacat gcacaccctg ggttccacgc acgccgtagt cccgatagcc ccgcccgagc
     3541 gctgcgggga cgaggcatcg gtcgcccgcc tcgccgggag cttcaaccgg tggggggagc
     3601 tgtgccgcca ggctggcgtg acgttctcgt accacaacca cgacttcgag ttcgcgccca
     3661 tggacggaac cacgatgtgg gatgtcctga tcagggagac cgaccccgat ctcgtgcatc
     3721 tggagctgga tctctactgg gtccgttacg gcggcgcgga cccggaaaga atacttcgtg
     3781 acctcggaga ccgtatctcg ctcgtccatc tgaaggacat ggcccctgac gagcaacgct
     3841 ccgacctgcc cgtgggagaa gggaccatgc cctggccgga attactgcag acggcagaag
     3901 aagctggcgt cgaatggtat gtggctgagc aggacaaccc cagagacgcc atcgaagacg
     3961 tacggatcag tctccaacac atgcgagagc tcgctagcga gtaggacccg cgagaagatt
     4021 tcccgcctga gtttctacac caaactcaag attggctgga gaccccgacg gggttcacct
     4081 gaccgctcag aaggtatccg gcaccaggca cgatggtagt caactccaga gcttgaagta
     4141 tgcagtaggt cgtagcggtc gcggcggaga tcaccggcag cccgcacgcc tgctccaccg
     4201 gctctatgct ggacagcgat ggcatctgca cgcacgcaga gagcaccagg gcgtcgcagc
     4261 cggcgaggtc gaccttcttc cagtgctcct tgaggttctc cgggtcaaga cggcggacct
     4321 cgaggttgtc cgagacttcc aggctcaacg catcctgcac catcacccca ctcgactcga
     4381 tgtactcaga tacgagctta gtgagtggtc gcatgtaagg ggcgaccagg gccacacggc
     4441 gtgctccgag atcttccagt gccttgagca acgctccggc agacgacaca acaggagccg
     4501 gcgctccctc ctcgtgcagc gcatccacga tctggtcctc cgccacacgg tggaacccgg
     4561 ctccctgggc catgatcgcg acaaggcaag ccgtggtgac cacgtcacac cgggcatcgg
     4621 ctaactctgc ggcacaacgg cccatctgcg cgttcatcgc ccgcaactcc tctggagtta
     4681 cgttcatcat gcgcatgcgg ctggaatgga aggtgaaccg ttctcccgaa cgttctgcct
     4741 cccgcgccag tatcatcctc gggatctccg tctccatcgt ggtgttggaa ctgggcacga
     4801 tcagcccaac gcgatatgta ttctgcatcc cttggatcac cgttatccct gtgtctttgc
     4861 gctatttcac cctcgcgtcg agtaacgcct gtataccata cgaccgaagc gcaggtaatc
     4921 gatgatacta atggggtgcg aggtcacggc ccgaaccgcg cgtgcgcgaa tcgaggagcg
     4981 gtgagggcgg gtcgcgatag gaagtgctcg attttcccgc ccgcaccatc gatacatcgc
     5041 tcatgccgtc cacgtatagt gtcagtaata acggacgaac aggcatgtag ttccctgatc
     5101 cagcagaaat accaatgcac tccggtacat gatcctgcac ggcacaatac ttttgaacgt
     5161 cgctgtagaa catggaggct aggatggctc tgcgacgact accttgggcc ctgagagttc
     5221 tatctctctt agtatctgtt tgggagcctt gtggtcggtg ataaagacgt cgaccctcga
     5281 gagggggatg atcctggcca gcgccacgac accgagcttg gagctatcgg cgaccacgac
     5341 gaccttctgg gccgtagcga cgagacagcc gttggtctcg gcctcggcgg tattgggtgt
     5401 cgtgaggccc gcgtccgggt gcacgccgtg tgccccgagg aagagcacgt cggcgttgag
     5461 cgtccgcaac gtcctgtcgg cgaaggggcc gacgagggcg tcggaaggcg tacggaacat
     5521 gccgccagag agcacgatac tctcccaccc gtgctcctgc agggtgagcg cgacgttggt
     5581 cgagttggta acgaacgtga ggtccttgtg accgggatac agggtggacg caacgtgcca
     5641 ggtcgtcgtg cccgcggaga aggccactgt gtcgccgtct tgtacgaact tctgggcggc
     5701 cctggctatg gcgagcttct cgggacgctt gagactgcgc ttctgctcga agtgcggctc
     5761 cgccgacctc gagacaggga tcgccccacc gtggacctta cgcagcaacc tgcggccaga
     5821 gagttcctcc aggtcgcgcc ggatcgtcat atccgagacc ttcagcttct ttgagaggtc
     5881 agcgacggat acgctcccga tccttcccaa ttcctccagg atcacaccgc gacgttgctc
     5941 cgccaacatt ttctgattgt ccaacatgaa ggcttagtat agccaaaatc ccggttttga
     6001 acccccggtt acgttctcgg cgccggctga tgcctcgtcc agctgacccc ctggagcgtc
     6061 ccggctacct gttccctgag gcggcgtcta tctttctgaa ctcgcggtca cacagcctgt
     6121 agacgatata ggcggtgatg ctgcctgtga taattacgag gaaaggtacg aaggccgtga
     6181 gccccgccat gaataatacg attacaaggc acaccagtgt ggtgggcagc cgtccgatgg
     6241 agagcagcag ggagttcttt atgaccgtcc tccagcctgt ctcgtagtga accatcacgg
     6301 ggaacaggaa gaccgagccg aaagcataca ccgaggacgc gagcacgaga acggacttga
     6361 gcacgacctc ggcaccggtg gagagctggc tggcaacgat gaagtcgagg aagagcgtca
     6421 ccccgaagaa ggtccacact gctcccacaa gcaggctctg cccgaggttc tccctgaagc
     6481 gcgtgacgaa cgtacgcgcc aggccgccct ctttgccgcg cacccagtcc cgcaccacgc
     6541 cgaacatggc ggccgtggag gggaagatcg ttacgaccgg cacgcacgcc acgagccaca
     6601 tgaggttgag caggaagaag tcagtcgcga cctccagcca cctgtatacc ctggtgtcga
     6661 gtatgttcat cgtcccacgc cagcgaactc gttgccgacc accgcaccat tatcccccag
     6721 aataggcgcc cccacagacg ctactacgcc cgtgcgacta tcctcacgaa tcctccgtct
     6781 tccccgcgag ataagatact gaaaccggcc ttcctcagga tgcgtgctac ttcgtcgggc
     6841 ccgagacgta gcttctggta ggcactcttt ctcgcctccc agccgtgccg gcccttctcg
     6901 aagatcacgt cgtgcacggt gacgttgtcc gcctcgtact ccaggaaagt cgccatgatg
     6961 cggtcgtcct ccatcctcac ggggatgatt ctgtccaccc ctttgagctc cgccgtgaag
     7021 tcgcggaact cgaagatcag ggttcccctt tggtccaggg cgccgcgcac gtcaccgacg
     7081 agcgcctcga cttcgcgcgg cgtcgccaga tgcgtcaacg tgtcacccat gcagacggcg
     7141 acctcgaaag gtccttctgc cgcgtacatt ccagggtcgc gaatgtcgcc gagcacggcc
     7201 aggatgtcgc ggcacccggc gcggctgcgc aactcttcga gaagcatgcc gctggtatct
     7261 acgcttagga ccgcgaagcc gagttcggcc agggcaatag actggaatcc ggggccgcac
     7321 ccgaggtcga gggccttgcc accggagcgg ggcgcgacgc cggcctcctc gaagaaagtc
     7381 aggttctccc gcaccttcga gacgtagtct ccgcgcatcc aggagtagtg cctggctagc
     7441 aggatctcgt aatgttcctc gacagaggac aaataggctt caggcatcag gtttcagcac
     7501 tctcgacgtg gcgcatgccg gaccaacgta gcatccggcc gaccgcctcg caatctgatc
     7561 cctccgaaaa acggcgtagc tgcaaaggga tcgtcgctca ctcttcgcga ggttgcttct
     7621 cgttcccgaa cgacctggtt cgaccccgga ttgcggatgc accaaccacc ttcagtctga
     7681 aagctgactg cgcggctggc cgttggccag acgctaatcg tgttcggctt ctgggtctgc
     7741 gggcttggct cccatgtcgc cgctatccat gtcggccatc atggcggcgg cgtattcgct
     7801 ctcctttcgc cgctcctctg ctggcagcag tgcggcgcac ggggtaccga atgccggttt
     7861 tgcgaacggg agatgcgtgt ccccgcgggc caggatgtga gaaccgttgg tcatcttcgt
     7921 atccgggtcc acgctcggcg cggtgtgcgg gtcgtcatcg tcgtggttcg cgccccactg
     7981 ggtgaaagag cgggcgttgc agtagtggcc gacgaaggcg cggcggaagc ggtcggtggt
     8041 gaagttcttc ttgctgcggt gcagcacgtg gccgccgaaa aagaccacgt cgcccgcctc
     8101 gaccggcgcg aggagttgcg ggtacctttc cgcgatcctg ctcaggtcgt tgttgtcgtc
     8161 gtcggggtcg ctcgccccgc tgatgtgctc tatccctccg aggcgctcgt cgccgtggcc
     8221 gtagccttct ttgggtggat agaccggctc gtgctggctc cccgaggcca tccacatcgc
     8281 gccgttgagc tcgtcggcgt cgtctatggc gaaccaggcg ccgcacagcg tgtcggggtg
     8341 ggtcgggatg tagtaggagt cctggtgcca gccctgcccc ggcttgcccg gcggcttgag
     8401 gaacaacatg gtctgcagcg cgagcacatc tgggccgatc agagcctcca gcacgtcgag
     8461 cacgcctgga tgaagcagat aacgctcgtg cagctccagc ttgcggtgca gcatgtgtac
     8521 gcgcaggaag tactgcgcct tctcttctgg ggaaaggtgt tccggtggtg cctccagcgc
     8581 ctgcgccgaa gtgcccgagt cggaaccggg gtccctcccg gccatcgtcg ccacctgctc
     8641 gggcaagcgt ccctgcatga gtagctccgt gtgctcgcgc aactcctcca cctcatcagg
     8701 cccgagaagt ccacgcacca ccagaaagcc ttgcttcctg aaagtcgtgt actcctcgac
     8761 cgagactctg tagtgctcgg gactcgcggt gctggatttt gatctcgtcg cgctagtgac
     8821 cacgcctcgc tccttcccac gaaggtgtct ttcatccccg gaaggggatt accagtcagg
     8881 atcatcgcac atgcgccgcg tgtggagaat gtcccggatt gaccggaaca tgcctgaaat
     8941 tgattaccgg agggcgggac tccgctgcag gagcctgggg gttggcggtg tggctccggt
     9001 ggcgaggcgc tttctcaggt cgcgcggcga gaggccgaag gcgtccttga agcggcggga
     9061 gaagtggaag ggactggaga agccgcagac cgtggagatc tccgccaccg tgtagttgct
     9121 gcgcgccagg aggacggcgg ccctgtcgag gcgggccagg cgcacggtct ccacagggga
     9181 tcttccggta gatctcttga ataggcggca cagatgttca gggctcacgc aggcggcgcc
     9241 tgcgaggtcc gagaggtgga tgcttgttga agagtcttcc tccaggctgg cgtggaggta
     9301 cgtcagggcc cgttcgacgg cttcgggcag ggactcttgc ggcacgtccc tgcccgcggt
     9361 ctcgccggtt acgaagctcg cgagcatgtg cgccatcgtc acggcgagca gatgaggatc
     9421 gccccgatcc gcccacgtaa gcaggtgccg gaacagcggc c
//
LOCUS       DATAIR010000051        13794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_47366, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000051 DATAIR010000000
VERSION     DATAIR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47366"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHM69_01335"
     CDS             complement(<1..237)
                     /locus_tag="VHM69_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEX2739060.1"
                     /translation="MDHRPPSIKVEKPWGRFEQYTHNLPCTVKIITVAPGGTLSRQYH
                     HHRDELWVILDEGARVELGEEVLEPGPGEKIFIPR"
     gene            383..544
                     /locus_tag="VHM69_01340"
     CDS             383..544
                     /locus_tag="VHM69_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739061.1"
                     /translation="MPRIEVEDAGTFDVEEGKRLVLAIEEDAGVDILHDVGPKPQHRQ
                     RVSLHGLDH"
     gene            629..994
                     /locus_tag="VHM69_01345"
     CDS             629..994
                     /locus_tag="VHM69_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2739062.1"
                     /translation="MPKLEVEGVGTFDVPENKRLVLAIEEDAGVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTKAELEVLKKRGLLGQVRLSCQTLSDHDKKVRVLMTVSSTGLDGP
                     GPHPEAHITPEPEWVDKPT"
     gene            1572..2687
                     /locus_tag="VHM69_01350"
     CDS             1572..2687
                     /locus_tag="VHM69_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2739063.1"
                     /translation="MTQETALRTKNGPQNREAIPVFDVDIHESFAALDDLVPHLDEPW
                     KGLVGRGEWKGFTQPFVYWTSGGSYWTPERADGEAPSNMQTSASDYGVMKDLLLDAFN
                     IERGVLTGYFYPVMLGEMQVEFASALASAYNDFQVERWLDKDDRFSGSIHVAPQDPLA
                     AAREIDRLGSHPQMVQVMLALGGLRRAYGDPFFFPIFEAAQRNGLAIATHHNTYAEGA
                     LGMGRYYVERHVLIPQVTMATLTSLVFNGVFDRFPDLRFILLEGGFTWLPHLLWRMDR
                     EYKSLRQEVPWVKKLPSEHVVGDGRVKLATQPTEDLTAEEWMKIVELIGSDEVLVFAT
                     DYPHFDFDSPTRALPNGLPEDLKRKILHDNAQAFYGF"
     gene            2677..3099
                     /locus_tag="VHM69_01355"
     CDS             2677..3099
                     /locus_tag="VHM69_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2739064.1"
                     /translation="MASSRAADNAVSATPSEGEAKQVVCREEELAPGQMRTLRNGGAP
                     VVVVCSAVGEYHAIRGICPHQGALLGRGHLWSLTVSEEPGVYGLAEKAEILRCPWHSF
                     DYDVKTGRCVSDPGLRVRTYPVSVEDGHIIVDLSKKRP"
     gene            3147..3773
                     /locus_tag="VHM69_01360"
     CDS             3147..3773
                     /locus_tag="VHM69_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2739065.1"
                     /translation="MPDEITSTTPGSRALAPTRDHEATLTDKRRDRGQGSPLRPALLG
                     VVPELVLAAVAVYLFVLARNFQYEPQPGQLGPGFWPQLLCAGIFVCALVRVAQKLRVR
                     AVPVSGGTKAEEGDHFRWQRLALALALVVGYVFGTVFLGYILATTLFFVAFVWLGGQR
                     RWYVVPLGLTVSLVFAFVFLKVVYVSLPSGVGVFDQLSVLLYRLIGVY"
     gene            3823..5379
                     /locus_tag="VHM69_01365"
     CDS             3823..5379
                     /locus_tag="VHM69_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2739066.1"
                     /translation="MSTFGGIIDAIVSMGPHTLGLIVVGVIAGLLVGVLPGLSFVMGV
                     LLLVPLTYNMGAQDAVVLLLALYVAGTYGGALTSILLKVPGESNDVVLLWDGHGMTRK
                     GQAAEALGWAAVSALGGGLAAWFVLVFAAEPFSSVALRLSSPEYFAIVLLGLASVLML
                     SETSVTTTIIATLVGMLISTVGIDSITGAVRYDFGTTILRDGVDYLAVMIGVYALTEV
                     ITRFSESFRGGTAQQPGRISTTIPGLRAIWHRSGSFFRGMLTGSLIGMVPGAGATIAS
                     FVSYGFEKQFGRFKGEVGESSPSGIVAPQTASTATVGGALIPLLVLGIPGSAAAAVLL
                     GVLQLHNVQPGPQIFTEQPELVYAIFGAFLLSLLLMFAVGIVGARPLVRVLGFPEAYI
                     SALVVIFAFLGAFALRQSMSDVWIMVAFGAVGFFMQRSDYPIAPLVLGAILGPLAETH
                     FRTAMISSGNDWTVFFTRPISGTLMAILCLVIGLLAYRRLRASRPREARIESGPPREA
                     YPPEQQDEER"
     gene            5413..6450
                     /locus_tag="VHM69_01370"
     CDS             5413..6450
                     /locus_tag="VHM69_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2739067.1"
                     /translation="MDQTKSTRPGRNVSWVRTIGNLLAGALVLTVLFSALVACSAQTR
                     GAGSAQAGEQSNRPVDMVVPFGPGGGADQLARITAPEMEKELDTQVPVVNTDGATGST
                     GMTSMLSAPPGESTAVLIQDTLATVAAGSASFELGELQGVCRLQEMPSGLFVKGDGPY
                     GSWDELAAAAKERPGELKVATVGESSADDIMLAALAQQGIEFRAVPFADPNERYNALL
                     GGAVDVLYEQPGDVLPNLESGQFEPVLIFSDKPVDGLEGNPVLSADVGVDVVLNQFRG
                     LVTSKEASPETVERLSDACSKVQEDEKFAEFQKSNYSMPGSYMNSKEFDKYLGEQLEQ
                     IESLGQRYGVS"
     gene            6501..7595
                     /locus_tag="VHM69_01375"
     CDS             6501..7595
                     /locus_tag="VHM69_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX2739068.1"
                     /translation="MAGDRTILKPLPPDKFIDYREDGDGEPVGATGDRAVNAETRWEA
                     MAGAGYLTPNELFFVRSHAPTPLVDRTTWELRVVGPGVERELILGYEDLLGLPRVSVL
                     QALECAGNGRRFFEELQGRAAPGTPWRLGAIGVAEWTGVPLREVLLRAGVRESAREVM
                     LEGLDAVRMRRPIPLAKALEEDTLLVFGMNGEELPPDHGFPVRALVPRWAAVASVKWL
                     GEIFVSEEPLYSPWNTDQYVLAGGRYGAEREPVTDQVPKSALELPCPAHLDAGRHTIT
                     GRSWSSSGAITLVEYSVDGGQWRPARLLEPNIAGAWVRWHFVWDATPGTHELRVRATD
                     ERGNTQPDDATWNELGYLYGGVVRHPAEIL"
     gene            7733..8080
                     /locus_tag="VHM69_01380"
     CDS             7733..8080
                     /locus_tag="VHM69_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739069.1"
                     /translation="MTLDEIGSKLKGLDETRRLAEVELTGLQAREKRAAELERDRDAL
                     LRSWASAVPEALEELRGQERNKVYRMLRLEVTPTTEGFAVTGALGGFIHSEMRLLRQM
                     YDLPHPVHRGRAG"
     gene            complement(8289..9254)
                     /locus_tag="VHM69_01385"
     CDS             complement(8289..9254)
                     /locus_tag="VHM69_01385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2739070.1"
                     /translation="MAVEFGVFVPQGWRMDLVGIEDPFEQYEAMTRVGKVADETGAYD
                     SIWVYDHFHTVPRPTMETTFEAWTVSAGLARDTKNVKIGQMVTCNGYRNPAYLAKVAS
                     TVDVMSNGRLLCGIGAGWYEHEWRAYGYGFPEVPVRMRMFKEACEIIHRMFTEDRPTF
                     EGEFYHIDRPINEPKGVQKPHPPIWIGGGGEKVTLKLVALWGDACNIGGDIETIKHKF
                     EVLKGHCENVGRDYEEITRSTSINVHLHEEGEDPEEATKEHRGSKSYEEYSEQFMVGT
                     PEQIVERLAPMVDAGVNYFITYLPRVAYDPAPVEHFAREVVPNVG"
     gene            complement(9336..10628)
                     /locus_tag="VHM69_01390"
     CDS             complement(9336..10628)
                     /locus_tag="VHM69_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739071.1"
                     /translation="MFLHLVCAACILVLAAGCSEDRPQTDASTISEKPRSTPSVPVYG
                     YTYGQATGNRVVDGKGQLPEANPVDVDLSGTPVWVAGVPLEEDTAWVVTYADGEVDAF
                     RLDGTSGEVGPWLTVPDVLPPDAPPLVVSEEDRLDLATLDGSSPLTHPVPTEGGLLGV
                     TPRGRLLSESGEQPTVSALRDARIVEGEGGSLAVLSDPTTRYAHGVLGDPFEAGSIKV
                     LEVGRDGYSVVGRIEPESGGVFEALSPLWFRPGDGEEELLAVTESTQRMGSRISVYSP
                     RGSLVAAGPFVGEPQSWRHLIAAGPFGPNGEMEIAAVRTPHVGGPVEFYRMNREDGKL
                     RLAASGGEYPSHTIYSRDLDAVRAGDLDGDGRWELLLPTSSYTTLEAVRHTRNGVEPA
                     WRVPLGGNLATNLASATDSKGRVVLAAGTLEGKLRTWR"
     gene            complement(10648..11172)
                     /locus_tag="VHM69_01395"
     CDS             complement(10648..11172)
                     /locus_tag="VHM69_01395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2739072.1"
                     /translation="MSEDNPPVSTGRGDDGTTTLLGAGRMGKDDLRIIVLGDVDEASS
                     FLGLARAEADEAVGGLLIELQRLLYRVMGDVAMPKEDNSVGEDALRYLDDALEEWRGR
                     TEIPKEFVIPGESRPGALLDVARTVVRRAERSLVAAGYAVEHPYAVRVVNRLSDLLFV
                     LARNADDKKTLSKG"
     gene            complement(11254..12387)
                     /locus_tag="VHM69_01400"
     CDS             complement(11254..12387)
                     /locus_tag="VHM69_01400"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2739073.1"
                     /translation="MSARPPTNAEETWGRVLGRRELNRALLERQMLLRRRELSAADAI
                     ERLVGMQAQVPNAPYVGLWTRLEAFHQEELECLITSRRAVRAPLMRATIHLVSARDGL
                     ALRPVVQPVLARAFSGSPFARNLAGVETGTIVEAGRTLLEERPRTRAELGKLMAERWP
                     DREAASLAHAVSFLAPLVQVPPRGIWGSGGQATWTTVEAWLGRPLDPDPSPDEMVLRY
                     LSAFGPAGVGDVRTWSGLSGLRAVIERLRPRLRAFRDENGRELLDLPDAPLPDPDTPA
                     PPRFLPEYDNVLLSHADRTRIIPEGQRLPLPPGGGGVRGTVLVDGFLRGTWRIQRTRG
                     MVTLEIQPFERLSKEDTDALAEEGERLVRFVAEPNEEFEIHFA"
     gene            12631..>13794
                     /locus_tag="VHM69_01405"
     CDS             12631..>13794
                     /locus_tag="VHM69_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2739074.1"
                     /translation="MLVRVGFPLRSEGHLGRAAALASRHPSYHVEGANGTARVSVDLD
                     LPSDWRLLDDFSALLRGEGDAEYAADGASLSADELFGGLRCFMRKQRSGTAAREWCTP
                     GSLADKQLFPCRQIRVYDNDRLTGNSWYAFGEMDDEAVFEVDKDAITERVLSDLGPCV
                     RCPILDLDSTAEIVARLPERIDPSRDPAWSYREDGDLSNWKVVKTRDGREEAAPPQDR
                     ESIIERLARSVRSGGAGVAAASGTAPGSRNVPATRYEDVGGMRQTIDLIREAVELPIT
                     HPEIFRKLHIRPHKGILFYGPPGTGKTLLARAVAHESGAHFIAVSGPEILNKFWGQSE
                     AKLRSIFNEARAKAPAIILFDEIDSFASSRDMMSESFEATLVSQLLSLMDGLNN"
BASE COUNT         2482 a         4272 c         4546 g         2494 t
ORIGIN      
        1 tcgcgggatg aatatcttct ctccgggccc gggttcgagg acctcttcgc ccagctccac
       61 gcgggctcct tcgtccagga tgacccacag ctcgtcgcgg tggtggtgat actggcgcga
      121 gagcgtgccg ccaggagcga cggtgatgat cttcaccgta cagggcaggt tgtgtgtgta
      181 ctgctcgaac cggccccagg gcttctctac ctttatggaa ggcggacggt gatccaagcg
      241 tgaccccctt tcgtgaatcg ccagtttagc cgatccaccc acggatttgg agacgtataa
      301 tcccgaaagt ccgcggcccg gcgttcgcgc cggtcctgcc ggcgtgatta gatgcgctca
      361 actgctaccg ggaggagggc aggtgcccag gatcgaggtc gaagacgctg gcacgttcga
      421 cgttgaggaa gggaagcggt tggtcctcgc cattgaggag gacgcggggg tggatatcct
      481 gcacgacgtc ggtccgaaac cacaacatag gcagcgagtt tcgcttcatg ggcttgatca
      541 ctgaggaagg caattagctg ctctctttgg aacattaaag atcctgccac tacactaacg
      601 gatgttccat ctctggaagg agggatgaat gcctaagcta gaggttgagg gcgtagggac
      661 cttcgacgtt cccgaaaaca agcgattggt tttggcgatc gaggaggatg caggggtgga
      721 tatcctgcac cgctgtggtt cttacgccag atgcaccacc tgccggatcg agtacctcga
      781 gggcgaacct gagaagatga cgaaggcaga actcgaagtt ctgaaaaagc gcggcctcct
      841 cggtcaggtc aggctctcgt gccagactct aagcgaccac gacaagaagg tgcgggtcct
      901 gatgaccgtg agctctacag gtctcgacgg tccgggtccg catcccgaag cccatatcac
      961 cccagaaccc gagtgggtgg acaagccgac ctaaggccgg ggttctccag agacctgtgc
     1021 acctgcgcga gggctcgaca tcaccgcttc aagtaatgaa atgatgagac cttaagatct
     1081 aagctcgcag tcgaccggag gaaggggtga gctgcagcag ccgttcgatg gcccgggtga
     1141 tctagaatca acacagacgc gtgggtcccg aaactcccag tcgcaagtga tcacgttccg
     1201 cgctttgctg aacgaaagtg aagccaaggt actattagat cgcgagtccc tttggaaccg
     1261 gcgagctctc cgttaggtat gtctgtcagc cgtggcggtc actatcctgc tatgggcatc
     1321 tgatactcaa gaccttgtaa cgagactgac tccccggaac gcgcctccaa ccgtaatctt
     1381 atcgataccg ggtgtacagc cccttgacac caacatcccg ttgagatagc ctcctgcgat
     1441 gttaactgaa atggtagaca aagtagacaa tagtaaggag agttgcttga atccgatccg
     1501 accggtgggg tgcgccaagg gactcgcgtg cgtggcgggg tgagctcgag tccatggagg
     1561 gaggacacac gatgacacaa gaaactgccc tgaggacaaa gaacggcccg cagaacagag
     1621 aagccatccc cgtattcgac gtcgatatac acgaatcgtt cgcggcactt gacgaccttg
     1681 tcccccactt ggacgagccc tggaagggcc ttgtgggtcg tggcgagtgg aagggcttca
     1741 cccagccgtt cgtctactgg acctcggggg gcagctactg gacgccggaa cgggcggacg
     1801 gcgaggcacc gagcaacatg caaacgtccg ccagcgacta cggcgtgatg aaggatctgt
     1861 tactcgacgc gttcaacatc gagcgcgggg tactcaccgg atacttctac ccggtgatgc
     1921 tcggagagat gcaggtggag ttcgcttccg ccctggcctc ggcttacaac gacttccagg
     1981 tcgaacgctg gctcgacaaa gacgacagat tctcgggcag catccacgtt gccccgcagg
     2041 acccgctggc agcggcccgc gagatcgaca gattgggctc ccacccccag atggtccagg
     2101 tcatgctggc gctcggcggg ctcagacggg cgtacgggga tcccttcttc ttcccgatct
     2161 tcgaggctgc gcagcgcaac ggcctggcca tcgccacgca ccacaacacg tatgccgaag
     2221 gcgcgttggg gatgggaagg tactacgtgg agcgccatgt gctcataccc caggtcacga
     2281 tggctacgct taccagcctg gtgttcaacg gcgtattcga ccgcttcccg gacctcaggt
     2341 tcatccttct tgagggtggg tttacgtggt tgcctcacct cttgtggagg atggatcggg
     2401 agtacaagtc gctcaggcag gaggtgccct gggtcaagaa gctccccagc gagcacgtag
     2461 taggcgacgg ccgcgtcaaa ctcgccaccc agcccaccga ggacctgaca gccgaagaat
     2521 ggatgaagat agtcgagctg atcggcagcg acgaggtgct ggtgttcgct accgattacc
     2581 cgcacttcga cttcgactcg ccgacgcgtg ctctgccgaa cggtctgccc gaagacctca
     2641 agcgcaagat actgcacgac aacgcgcagg ccttctatgg cttctagtcg ggcggctgac
     2701 aacgctgttt cagctacccc gagtgagggt gaggcgaagc aggttgtctg cagggaagag
     2761 gaactcgccc ccgggcagat gcgaacgctc cggaacggcg gggcaccggt cgtcgtggtg
     2821 tgcagcgccg tcggcgaata ccacgcgatc cgcggcatct gcccgcacca gggggcgttg
     2881 ctgggcaggg gccacctgtg gtcgctcacc gtttcggaag agcccggcgt ctacggtctg
     2941 gccgagaaag cggagatcct ccgctgcccc tggcatagct tcgattacga cgtgaaaacc
     3001 ggaaggtgcg tgagcgaccc cgggttgcgc gtcaggacgt acccggtctc cgtagaagac
     3061 gggcacatca tcgtcgacct gagcaagaaa cgcccctgag cgcgggccgc gcagaagtga
     3121 tgaggatcgg acggaggtag gacggaatgc ccgacgaaat cacatcgact actcccggct
     3181 cgcgagcgct cgcgccgacc agggaccatg aagcgacgct cacagacaag cgccgcgatc
     3241 ggggccaggg ttcgcctttg cgcccggctc tgctcggggt ggtgcctgaa ctggtgctcg
     3301 cggccgtcgc tgtgtacctg ttcgtactcg caaggaactt ccagtacgag ccgcaacccg
     3361 gtcaactcgg tcccggcttc tggccccagt tgctgtgtgc cggcatcttt gtctgcgccc
     3421 tcgtgcgggt cgcccagaag ctccgcgttc gcgccgtgcc ggtgtccgga gggacgaaag
     3481 ccgaggaagg agatcacttc cggtggcaac gtcttgcgct ggcgctggcg ctggtcgtcg
     3541 ggtacgtgtt cggcaccgtg ttcctaggct acatcctggc caccaccctg ttcttcgtcg
     3601 cgttcgtctg gctgggggga cagaggcgtt ggtacgtggt gccgctcggg ctgacagttt
     3661 cgctcgtctt cgccttcgtt ttcctcaagg tcgtgtacgt ctcgttgccc tcaggggtag
     3721 gggtcttcga ccagctcagc gtgttgctgt accggttgat cggggtgtac tgatcggcct
     3781 actggtggac agtcacggtt cccataggaa ggagcgaact ggttgagtac ttttggcggc
     3841 ataatagacg ccatcgtctc gatgggccct cataccctgg gcctcatagt ggtcggggtt
     3901 atcgccggcc tgctcgtggg tgtgctccct ggtctcagct tcgtgatggg tgtcctgctc
     3961 ctggttccgc tcacctacaa catgggggca caggacgcgg tggtcctgct gctggcgctg
     4021 tacgtcgccg gtacctacgg tggggcgctc acctcgatcc tgctaaaggt ccccggggag
     4081 tccaacgacg tggtgctgct gtgggacggc catggcatga cgcgcaaggg acaggctgca
     4141 gaggcgctgg gatgggccgc cgtctctgcc ctgggcgggg ggttggcggc ctggtttgta
     4201 ctcgtcttcg ccgctgaacc gttctcgtcc gtggcgctcc ggctctcctc gcccgagtac
     4261 ttcgccatcg tcctgctcgg tctcgccagc gttctcatgt tgagcgagac ctcggtcacg
     4321 acgacgatca tagcgacgct tgtgggcatg ctcatctcca ccgtggggat cgactcgatc
     4381 accggcgccg tccgctacga cttcggtacg accatactca gggacggcgt cgactacctg
     4441 gccgtcatga tcggggtgta cgcgttgacc gaggtgatca cacgcttcag cgagagtttt
     4501 cgtggcggca cggcgcagca gccgggccgc atcagcacca ccatccccgg cctgcgagcg
     4561 atatggcaca ggagcggcag cttctttcgg gggatgctga ccggaagcct catagggatg
     4621 gtgccgggag ctggggccac catcgcttct ttcgtgtcct acggcttcga gaagcaattc
     4681 ggcaggttca agggagaggt gggggagtcc tccccgagcg gcatcgtggc cccccagacg
     4741 gcttcgacgg ccacggtggg cggggcgctc atacccctgc tcgtgctcgg gataccgggc
     4801 agtgcggcgg cggcggtgct cctcggcgtc ctgcagctcc acaacgttca acccggacct
     4861 cagatcttca ccgagcagcc cgaactcgta tatgcgatct tcggggcgtt cctgctcagc
     4921 cttcttctca tgttcgccgt ggggatcgtc ggggccagac ccctcgtgcg cgtgctcggc
     4981 ttcccggagg cttacatctc ggcgctcgtg gtgatcttcg cgttcctggg cgcgttcgcg
     5041 ctgcgccagt cgatgtcgga tgtgtggatc atggtcgcct tcggggcggt aggtttcttt
     5101 atgcaacgca gcgattatcc catagcgcct ctggtgctgg gagcgatcct gggacccctc
     5161 gccgagaccc atttcaggac ggcgatgatc tcaagcggca acgactggac cgtgttcttc
     5221 acgcgcccta tcagcggaac gctcatggcg atcctgtgcc tggtcatagg gttactcgct
     5281 tacaggagat tacgggccag tcgtccgcgc gaggcccgca tcgagagcgg ccctcctcgg
     5341 gaggcatacc cgcccgagca gcaagacgaa gagcggtaag agagaccgga tacaacgatg
     5401 ggaggaacga tattggatca gacaaagagc accaggcccg gaagaaacgt ttcgtgggta
     5461 cggacgatag gtaatttgct ggcaggtgcc ctggtgttga ccgtgctgtt ctcggcgctc
     5521 gtcgcctgta gcgcgcagac gaggggagcc ggcagcgcac aggctggaga acagtcaaat
     5581 cgaccggtcg acatggttgt accgttcgga cctggtggcg gagccgacca gctggcgcgg
     5641 atcacagccc ccgaaatgga gaaggagttg gatacgcagg tcccggtggt gaacaccgac
     5701 ggcgccacgg gcagcaccgg aatgaccagc atgctctccg cccctccagg ggagtcgacg
     5761 gccgtcttga tccaggacac gctggccacc gtcgcggccg gcagcgcctc cttcgagctc
     5821 ggcgagctgc aaggcgtctg ccgcctgcag gagatgccct ccggcctctt cgtcaagggc
     5881 gacggcccgt acggaagctg ggacgagctc gcggcggccg cgaaggagcg gcctggcgaa
     5941 ctcaaagtgg cgacggtagg ggagagcagc gccgacgaca tcatgctggc cgccctcgcg
     6001 cagcagggga tcgagttccg cgccgtaccg ttcgccgatc ccaacgaacg ctacaacgct
     6061 ctcttgggag gagcggtcga cgtcctctac gagcagcctg gagacgtgct gccgaacctc
     6121 gaatcggggc agttcgagcc agtactcatc ttctcggaca aaccggtcga tggcctggaa
     6181 ggcaaccccg tcctgagcgc cgacgtcggg gtagacgtgg ttctgaacca gtttcgcggc
     6241 ctcgtgacga gcaaagaagc gagccccgag accgtagaga ggctctctga cgcctgctcc
     6301 aaggtgcaag aagacgagaa gttcgcggag ttccagaaga gcaactactc gatgccaggc
     6361 agctacatga actccaagga gttcgacaag tacctcggcg agcagctcga acagatcgaa
     6421 agcctcgggc aacgatacgg ggtgagctga tggcagcacc tcgacgggga accgtgccga
     6481 ccgaggtgcg gagacaaaca atggctggag accgaaccat cctcaagccg ttgccgccgg
     6541 acaagttcat cgattaccgc gaagacggcg acggagagcc ggtcggcgcc accggcgatc
     6601 gagctgtcaa cgccgagacg cgttgggagg cgatggcggg cgcgggctac ctgacgccga
     6661 acgagctgtt cttcgtacgc agccacgctc cgaccccgct cgtagaccgc acaacctggg
     6721 agctgcgggt ggtcggtccc ggcgtcgagc gcgagctgat cctcgggtac gaagatctgc
     6781 tggggctgcc ccgcgtctcg gtattgcagg cgctggagtg cgcaggcaac gggcggcgtt
     6841 tcttcgagga actgcagggc agggccgcgc cgggaacgcc gtggaggctc ggggcgatag
     6901 gggtcgccga atggacgggc gtgcccctgc gcgaggtcct gttacgggcc ggcgttaggg
     6961 agagcgccag ggaggtgatg ctcgagggcc tcgacgccgt caggatgcgc aggcccatac
     7021 ccctggccaa ggcgctcgag gaggacacgc tgctggtctt cggcatgaac ggcgaggagc
     7081 tgccaccgga tcacggattt ccggtcaggg cgctcgtccc ccgctgggcg gcggtggcga
     7141 gcgtcaagtg gctcggcgag atcttcgttt ccgaagaacc tctctactca ccgtggaaca
     7201 ccgaccagta cgtgctggct ggcggaaggt acggggcaga gagggagccc gtgacggacc
     7261 aggtcccaaa gagcgctctc gagctcccat gtccggcgca cctcgacgcc ggcaggcaca
     7321 ccatcaccgg gcgttcatgg tcgtcgtctg gtgccatcac cctggtcgag tacagcgtgg
     7381 acggcgggca gtggcggccg gcgcggcttc tcgaaccgaa catcgcaggg gcgtgggtgc
     7441 gctggcactt cgtctgggac gctacgccgg ggacccacga gctacgggtt agggccacgg
     7501 acgagagggg aaacacccag cccgacgacg cgacctggaa cgagctcgga tacctatatg
     7561 gcggcgtcgt caggcacccg gccgagattc tgtgagggac atgctaagag gtgacatctc
     7621 gtcgcctaaa tgcgcgtaat ccaaacggga gtagcaaggt ccatcgccga cgcacctcag
     7681 ctgcacaact tctgcgattg agtttcggga ggttagcgag ccggcagggc tcatgaccct
     7741 cgacgagatc ggctcgaagc tcaagggtct ggacgagacc cgcaggctcg ccgaggtcga
     7801 actcacgggc ctgcaagcgc gagagaaacg cgctgcggag ctggagaggg atcgcgatgc
     7861 acttctcagg tcttgggcca gcgcggtgcc ggaggccctc gaggagctga ggggccaaga
     7921 gagaaacaag gtctatcgga tgcttcgcct cgaggtgacg cctacgaccg agggtttcgc
     7981 agtgacggga gccctgggag gttttataca ctcagagatg cggctcctac gccagatgta
     8041 cgacctgccg catccagtac atcgagggcg agccggataa gataacgaag gccgggctcg
     8101 aagttctgaa atagcgcgac ctcctcgggc aggcacgcct ctcgtgccag gccctctgcg
     8161 accacgacat aaagttgcgc gtcctgctga ccgtcacctc cacaggcctc gacggcccgg
     8221 gaccgcatcc cgagtcgcat ataaccccgg aaccagaatg ggtggacaag ccggcgtaag
     8281 ggcggccctc acccgacgtt ggggacgacc tccctggcga agtgctcgac cggtgccgga
     8341 tcgtaggcga cccgcggcag gtaggttatg aagtagttga ccccggcgtc gaccatcggt
     8401 gcgagacgct cgacaatttg ctccggcgtt cctaccatga actgctcaga gtactcctcg
     8461 tagctcttgg agccgcgatg ctctttcgtg gcttcctccg ggtcttcgcc ttcttcatgg
     8521 aggtggacgt ttatggaagt ggagcgtgtg atctcctcgt agtccctgcc gacgttctcg
     8581 cagtggcctt tgagtacctc gaacttgtgc ttgatggtct cgatatcgcc gccgatgttg
     8641 caggcgtcgc cccacagtgc aacgagcttc agggtgacct tttcgcctcc gcccccgatc
     8701 cagatcggcg ggtgaggctt ctgcacacct ttcggctcgt tgatgggcct gtctatatgg
     8761 tagaactcgc cctcgaaggt cggtctgtcc tcggtgaaca tgcggtggat gatctcgcac
     8821 gcctccttga acatgcgcat tctcactggt acttcgggga acccgtagcc gtaggcgcgc
     8881 cactcgtgct cgtaccagcc tgcccctatg ccgcagagga gcctgccgtt gctcatcacg
     8941 tccaccgtcg atgcgacttt tgcgagatag gcagggttgc ggtagccgtt gcacgtgacc
     9001 atctgcccga tcttgacgtt cttcgtgtcg cgggcgagcc ctgcgctcac ggtccaggcc
     9061 tcgaaggtgg tctccatcgt cggcctggga acggtgtgga agtggtcgta gacccagatg
     9121 gagtcgtatg ctcccgtctc gtcggcgacc ttgcctacgc gagtcatcgc ttcgtactgc
     9181 tcgaacgggt cctcaatacc gacaaggtcc atccgccagc cctgcggaac gaagacgccg
     9241 aattcaaccg ccataagtga tttccccctg acgtttctcc tggcaactaa attctaggat
     9301 attctccgcc agccctccgc gcaacgcgcc cttcgctagc gccaggtcct caacttccct
     9361 tccagcgtgc ctgcggcgag gacgacgcgg cctttggagt cggtggctga ggccaggttg
     9421 gtcgcgaggt tcccgccgag cggcactctc caggccggtt cgaccccgtt cctcgtgtgg
     9481 cggacggctt cgagggttgt gtatgaggag gtaggaagca gtagttccca gcggccgtcg
     9541 ccgtcgaggt ctccggcgcg gacggcgtcg aggtcgcggc tgtagatggt gtgggaaggg
     9601 tactcgccac cactcgcggc gaggcggagc ttgccgtcct cacggttcat tctgtaaaac
     9661 tccacgggac cgccgacgtg cggggtgcgg acggcggcga tctccatctc cccgttcggg
     9721 ccgaaggggc ctgccgcgat gagatgcctc caactctgcg gttccccgac gaacggtccc
     9781 gccgcgacca gcgatccgcg aggcgagtag actgagatcc tgctccccat cctctgcgtg
     9841 ctctccgtta cggccagtaa ctcctcctca ccatcgccgg gacggaacca cagcggcgag
     9901 agagcctcga agacgccacc tgactccggc tcgatgcgac cgacgaccga gtagccgtcg
     9961 cgaccgacct caagcacttt tatgcttccc gcctcgaatg ggtcgccaag tacgccgtgg
    10021 gcatagcgcg tggtcgggtc ggagaggacc gcgagcgaac cgccctcacc ctcgacgatg
    10081 cgcgcatccc tgagcgcgga taccgtgggt tgctctccag attcggagag tagccgcccg
    10141 cgcggagtaa cgcctagcag gccgccctcg gtcggtacag ggtgggtgag cggggaactg
    10201 ccgtcgaggg tggcgaggtc gaggcgatcc tcctcggata ctaccagggg cggcgcgtcc
    10261 ggcgggagaa cgtcggggac ggtgagccag ggcccgacct caccggatgt gccgtcgagc
    10321 ctgaatgcgt ccacttctcc gtctgcgtat gtgaccaccc acgcggtgtc ttcctcgagc
    10381 ggcactcccg ctacccacac cggggtgcct gagagatcca cgtccacagg attcgcctcg
    10441 ggtagctgtc ccttgccatc cacgacccgg ttgcccgtgg cctgcccata ggtgtaaccg
    10501 tagacgggga cggagggagt gctccggggc ttctcgctga tcgtggaggc gtcggtctgt
    10561 ggacggtctt cggagcatcc cgcggcgagg accaggatgc aggccgcgca gacaaggtgg
    10621 aggaacatcg tcccccggtt caggacccta gcccttcgag agggtcttct tatcgtcggc
    10681 gttgcgcgcg aggacgaaga ggaggtcgga gagacggttg acgacgcgta cggcgtaggg
    10741 gtgctcgacc gcgtatccgg cggcgacgag gctccgctcc gccctgcgaa ctacggtgcg
    10801 ggcgacgtcg aggagcgcgc ctggcctgct ctcgcctggg atgacgaact ccttggggat
    10861 ctcggttctg cctcgccact cttcgagcgc gtcgtccagg tacctcaggg catcctcgcc
    10921 gacagagttg tcctccttag gcatcgccac gtcgcccatg acgcggtaga ggaggcgctg
    10981 cagctcgatc aagagcccgc caaccgcctc gtccgcctcg gcgcgggcca ggccgaggaa
    11041 ggagctagcc tcgtcaacgt cgccgagcac gattatcctg aggtcgtcct tgcccatcct
    11101 tccggccccg agcagggtcg tcgtaccgtc gtcgcccctg cctgtcgaga ccggcgggtt
    11161 gtcctcgctc aagatgatct cctttcctcg tcggaatgtg ccagggcacg gtagcccgga
    11221 aggtcacgta gcgcaagcca gggacgagcg tttctaggcg aagtggatct cgaactcctc
    11281 attcggttcg gcgacgaagc ggacgaggcg ttccccttcc tcagcaaggg cgtccgtatc
    11341 ctctttagag agccgttcga agggttggat ctcgagcgtc accatcccgc gagttcgctg
    11401 tattctccac gttcctctca ggaagccgtc tacgagcacg gtgccgcgga ctccaccgcc
    11461 gccggggggc agaggcaggc gctggccttc agggataatg cgggtgcggt ctgcgtggga
    11521 gagcagcacg ttatcgtact ccggcaggaa gcggggtgga gccggggtgt cggggtcggg
    11581 caaaggagcg tcagggaggt cgagcaactc cctgccgttc tcgtcgcgga aagcgcgcag
    11641 acgtggcctg aggcgttcga tcaccgcgcg cagcccgctc aggcccgacc aggtccggac
    11701 gtctccgacg ccggcaggac cgaacgcaga caggtagcgc aggaccatct cgtcaggcga
    11761 gggatctgga tccagcggtc tgccgagcca cgcttcaacg gtggtccacg tggcctgtcc
    11821 gccggaaccc cagatccctc gtggtggcac ctggaccagc ggagccagga agctgacagc
    11881 gtgggctagc gaagcagctt cacgatcggg ccacctctcg gccatcagct tgccgagctc
    11941 tgcgcgggtg cgcggccgtt cctccagcag cgtccgtcca gcctccacga tcgtgcctgt
    12001 ctccacgccg gcgaggttcc tggcgaaggg gctgccggag aaggcacggg cgaggacggg
    12061 ttgcacaacg ggacgcagcg ccaggccgtc gcgcgcgctg acgaggtgaa tggtcgcgcg
    12121 catcaagggg gcgcgtaccg ctcggcgaga ggtgatcaaa cactccagct cttcctggtg
    12181 gaaggcctcg aggcgcgtcc acaacccgac gtacggcgca ttgggtacct gggcctgcat
    12241 gccgaccaga cgctcgatgg cgtccgcggc ggatagttcc cggcggcgca ggagcatctg
    12301 ccgctccagc agggcgcggt tgagctcgcg cctgccgagc accctgcccc atgtctcttc
    12361 ggcgttggtt ggcggtcgtg cgctcatacc catcctttca cgcgccggtt gggcagcggt
    12421 ccgggcccgc gcaggtcgtc gaaagactct acctctcggg ctagcgatcc caccacggtc
    12481 atatcgtacc ggcgaaacag gtcataagcg ttccttattg gtcaggatct ccggaaggag
    12541 gcgacgcgcg tattacagga caccggcgga tgagtgaact gacatctatc tccgacgatt
    12601 tcgggtggcg tgggttagaa tctgcgggcc atgcttgtga gagtcggttt cccgttgaga
    12661 tccgaaggcc acctcggccg ggctgcggcg cttgcgtccc ggcatccgtc gtaccacgtc
    12721 gagggcgcga acggcacggc gcgggtctcg gtcgacctcg acctcccttc ggactggagg
    12781 ctcctcgacg acttctcggc gctcctgcgc ggcgagggtg acgccgagta cgcggccgac
    12841 ggggcctcgc tctccgctga tgagctcttc gggggcctga ggtgctttat gcgcaaacag
    12901 cgctcgggga cggctgcgag ggagtggtgc acgccaggtt ccctggcgga caagcagctc
    12961 tttccatgcc gtcagatccg ggtctacgac aacgaccgtc tgacgggtaa ctcatggtat
    13021 gccttcggtg agatggatga tgaggccgtc ttcgaggtgg acaaggacgc catcaccgag
    13081 cgcgtcctct cggaccttgg cccgtgcgtc aggtgcccca tcctcgacct ggactcgacc
    13141 gccgagatag tcgcccgcct tcccgagagg atagacccga gcagggaccc tgcatggagc
    13201 tacagggagg acggcgacct ctcgaactgg aaggtcgtga agacgcgtga cgggcgcgag
    13261 gaggcggccc cgccccagga tagggagagc atcatcgagc gcctggcgcg cagcgtgagg
    13321 agcgggggag cgggggtcgc ggcggcaagc ggcaccgcgc cgggcagccg caacgtgccc
    13381 gctacccgct acgaggatgt cggcgggatg cgccagacca tagacctgat tcgcgaggct
    13441 gtggagctac ccataaccca ccccgaaatc ttccgaaagc tacatatcag accccacaaa
    13501 ggcatcctct tctacggtcc gccggggacg ggcaagacgc tactggcgag ggccgtcgcc
    13561 cacgagagcg gggcccactt catagccgtc tcggggccgg agatcctcaa caagttctgg
    13621 ggacagagcg aggcgaagct tcgctccata ttcaacgaag cgcgcgccaa ggcgccggcg
    13681 atcatcctct tcgacgagat agactccttc gcctcatcgc gcgacatgat gagcgagagc
    13741 ttcgaggcga ccctcgtctc ccagctcctc tccctcatgg acgggttgaa caat
//
LOCUS       DATAIR010000052         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_279706, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000052 DATAIR010000000
VERSION     DATAIR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279706"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VHM69_01410"
     CDS             <1..553
                     /locus_tag="VHM69_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX2739075.1"
                     /translation="RKLLSGCPSSVTTNYVSLEDGYYRLSPSVRVSTDVDEFDERHEK
                     GLQLEKERRMMEAACEYERAIEIYRGDYLVEDLYEDWTMVERERLTNAYMDMLGRLAI
                     HYMEVEQHQESIRACYKVLEKDRCHEDSYRLLMRCYARLGLRARALHQYRMCEQILGQ
                     EYATSPSPETRSLYVSLLRDESA"
     gene            complement(802..2280)
                     /locus_tag="VHM69_01415"
     CDS             complement(802..2280)
                     /locus_tag="VHM69_01415"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX2739076.1"
                     /translation="MKADLTRSTFDQHKQFTRVLMQQGRVQLDSDWNEQTAILLHYMQ
                     TLAADIIGPHGGPQDNFGFGIIFDPTSLPEEERETLSEEEKRVLESLEDRDFAIGKGR
                     YYVDGMLCENESYVTYTDQPYFPGVEPPEEDRYLVYLDVWERHITSIEDPDIREVALG
                     GPDTATRAKLVWQVRVDSVPDESVDKEGFDARFTLDRDSVRANWRDGWVRRWQPDNRG
                     MLKAKGREGVEDDSDPCIVSPEARYRGAENQLYRVEIHTGGEISRDDGSDEKSLSPTG
                     METAGVPTFKWSRDNGSVTYPIRDLDGSVATLESMGRDDYLGLKVGDLVEIVDDDYVL
                     QGRAEPLLRVEEVDATTLRVTLDGQPPSDVGRHPTKHPLLRRWDHGSGGSPNGSSSYD
                     TLPLREDTWLTLEDGIQIYFKPADAQGPNNCYRTGDYWLIPARTATGDVEWPGDVGDP
                     QAQPPHGIDHHYAPLAIVSNGSPPSDCRCRFDRLGVCLSDGE"
     gene            complement(2292..2894)
                     /locus_tag="VHM69_01420"
     CDS             complement(2292..2894)
                     /locus_tag="VHM69_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739077.1"
                     /translation="MCINIEHSIVGSIQINQDEVQAEPVRILIRDSVLDATSPEREAL
                     GSPDWRVAHARLTVARSTVFGRVQAHAIDLAENSIFEGRVEVARRQLGCMRFCYVTPH
                     SRTPRRFNCQPDLAEQAADPVTVEILRVRPRFDDVRYGTPTYCRLAESCAEEIKRGAE
                     DGSEMGVFHDLFQPQREANLRARLDEYTPAGTDAGLIFAS"
     gene            complement(2849..4735)
                     /locus_tag="VHM69_01425"
     CDS             complement(2849..4735)
                     /locus_tag="VHM69_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739078.1"
                     /translation="MSPSADRLYELLPAVYRRRDEEQGQPLRSLLRVITEQVDVVEED
                     IDRLYENWFVETCEDWVVEYLGDIIGYRPVYEAGNSGSSQGAGRGSILVPRREVANTI
                     RYRRRKGTLALLELLASDVAGWPARAVEFYNLLGLTQAINHVTLMRGRTVDVREEIAL
                     DRLDGPFDELAHTADVRRINSHRSLGRYNIPTVGLFVWRLKSYPVTETPAYYREDAGV
                     HCYTFSVLGNDAPLYTRPEPDPDPTHIAEVMNVPAPIRRRAFDAHKTDYYGPGKSLEI
                     LEQDSRRPVSPDRIVAADLTDWLYRPRRGQVAVDPELGRIAFNPREVPDRVWVSYHYG
                     FTADIGGGEYARSLPQPPDHLLLDRHDLKDPAALIGRLQEPRDPLSRYLRDRFSEEGK
                     QMLEEQHGSPSEEHQKAMVDELNRLLQGESLYAAQRFEHVTLPENIMRLVGQDLEGRG
                     LTRLNRLLLEETYPRELTKSFALYRVGPGEAHRRINGAVRQWREERPRHAVIEITDSG
                     VYVEQIYIPLREYQTLQLRAADGVRPVIRLLDWHTDLPDALSVKGGRGSRFTLDGLLV
                     TGRGCRRKESWPDLPSATPPSCPAGDCTETANRAGPPNRACNCSTPRTCASTSSTASS
                     APFR"
     gene            complement(4732..>6505)
                     /locus_tag="VHM69_01430"
     CDS             complement(4732..>6505)
                     /locus_tag="VHM69_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX2739079.1"
                     /translation="LEPGRWLFVTGERDDLPGVRASELVMLAGVEQGVRLVSLTDENL
                     PRVGEATALRLTGRGSTGPGGLDLTISARRAAGGSVSGYAHLVGGATGEVVGIVPPDD
                     ERDFWCINVRRTDTGPNSGDQRINWYVRDAGDGITAFDEISYVTSIGGDCENLSAPTG
                     SWLRLVKGDFHVSAEEPTGEKVHSTIILAGDLAYPYKRETVRIYGNVVNATHGETRPE
                     VLGSGDASEAFQKFSLNQSPLTHVAATTPSGVESTLEVYVNDIRWHEAEHLSGLQPTD
                     RRFITQTDDLDEVTVVFGDGQRGARLPTGVENVRAVYRTGIGKAGNVAEGQISLLATR
                     PLGLKGVINPQPATGGADRETREGARRNAPLGVKVLDRLVSVRDYEDFAHAFAGIGKA
                     SSTRLSDGRQQLVHLTIAGADDIPIAETSDLYRNLLQALRRFGDPQQPIRVEVRELLL
                     LIVSAGVRLLPDYLWEPVEREIRAALLETFGFEQRDLGQDALLSEVISTIQHVPGVAY
                     VDVDTFGGIPEKTVDVATGLRRVLLPSEIEAEITRLTQSEEQPYQRVRVDLAGSSGIS
                     IRPAQLAFLTPLVPDTLILKEITA"
BASE COUNT         1070 a         2047 c         2110 g         1278 t
ORIGIN      
        1 gagaaaactc cttagcggat gcccttcttc tgtcaccacg aactacgtct ccctggagga
       61 tggatactac aggctctctc cgagcgtcag ggtgagcacg gacgtggacg agttcgacga
      121 gcgtcacgag aaaggtctgc agctggagaa ggagcggcgg atgatggagg cggcctgcga
      181 gtacgagagg gcgatcgaga tctacagggg agactatctg gtagaggacc tctacgaaga
      241 ttggaccatg gtagagcgag agcggctcac caacgcctac atggatatgc tgggtaggct
      301 cgccatccac tacatggagg tcgagcagca ccaggagagc atccgtgcct gttacaaggt
      361 gctcgagaaa gaccgttgcc acgaagacag ctataggctt ctgatgcgct gctacgccag
      421 gctggggttg cgagcgaggg cgctgcacca gtacaggatg tgcgagcaaa tactcgggca
      481 ggagtacgca acgtcaccat cgccggagac caggtccctc tacgtgagcc tcctgagaga
      541 cgaaagtgcg tgagaattgc tagatcaggc tcttcgtagg gatccctgag cctaaggtcg
      601 aagtgccgga acctgccagt agatcccgaa cagtagcagg accatgatca ccttcggtcg
      661 ctgacatact cctggaacac ggtacggacg tgaccacagc cctgatctaa gcaggacacg
      721 ccgtacccga gatagccttc gaccgcaacc gctctcgata cagcgaagct tggcatcttc
      781 tgaacaggtg ctgccggatt cctactcgcc gtccgagagg catacaccga gccggtcgaa
      841 cctgcagcga caatcgctcg ggggggagcc gttcgagact atcgccagcg gcgcatagtg
      901 gtggtctatg ccgtgtggcg gctgcgcctg tgggtcgcca acatcccccg gccactccac
      961 gtcgccggtg gccgtgcgcg caggtatcag ccagtagtcg ccggtccggt agcagttgtt
     1021 cggtccttga gcgtcagcgg gtttgaagta gatctggatg ccgtcctcca gggtgagcca
     1081 ggtgtcttct cttaggggca gggtgtcgta gctcgaggaa ccgttcggcg aaccgcctga
     1141 gccgtgatcc cagcgacgca gcagcggatg cttcgttgga tgtcgtccga cgtcggaggg
     1201 cggctgtccg tccagcgtta cccgcaaggt ggtggcgtcg acctcttcga ccctaagcag
     1261 cggttcggcg cgcccctgga gaacgtaatc gtcgtccact atctctacca ggtcgccgac
     1321 cttcaggcca aggtagtcat cccgacccat actttcgagg gtggccacac tgccgtccag
     1381 gtcgcgtatg ggataagtta cggagccgtt gtcccgtgac cacttgaagg taggtacgcc
     1441 ggcagtttcc atccctgtgg gtgacaggga cttctcgtcg gagccatcgt cgcgcgagat
     1501 ctcaccccct gtgtggatct ccacgcggta gagctggttt tcggcgcccc ggtaacgcgc
     1561 ctcgggggag acgatgcaag ggtcggagtc gtcttcgacc ccctctctgc ccttcgcttt
     1621 gagcatgccg cgattgtccg gttgccatct ccgcacccat ccatcacgcc agttcgcccg
     1681 gacgctgtct ctatcgagcg tgaacctggc atcgaacccc tctttgtcaa cggactcgtc
     1741 agggacgctg tctactctca cctgccacac caacttggcc cgcgtcgccg tgtccgggcc
     1801 gcccagcgcc acctcgcgga tgtcggggtc ttcgatggac gttatgtgac gttcccacac
     1861 gtcgagatag accaggtagc ggtcttcctc cggtggctca acgccgggga agtagggctg
     1921 atctgtatac gttacgtacg actcgttctc gcagagcatg ccgtcaacgt agtagcgccc
     1981 tttgccgatg gcgaagtctc tgtcctccag gctttccaac actctctttt cctcttctga
     2041 gagagtctct ctttcctctt cggggaggga ggttgggtcg aagatgatcc cgaacccgaa
     2101 gttgtcttgc ggcccgccgt gcggcccgat gatgtccgcc gccagggttt gcatgtagtg
     2161 cagcaggatg gcggtctgct cgttccagtc ggagtccagt tggacccttc cctgttgcat
     2221 gagcacgcgg gtgaactgct tgtgctggtc gaaggtgctt cgggtgagat ctgccttcat
     2281 gtcatgcctc cttagctcgc gaagattaag cctgcgtctg ttcctgctgg cgtgtactcg
     2341 tcgaggcggg cgcgcaggtt cgcctcccgc tgtggttgga acaggtcgtg gaacacaccc
     2401 atctccgacc cgtcttcagc gccgcgtttg atctcctcgg cgcacgactc ggcgagccgg
     2461 cagtaggtgg gcgtgccgta gcgtacgtcg tcgaaccgcg gcctgacacg aaggatctct
     2521 accgtgaccg ggtccgccgc ctgttctgcc aggtccggct ggcagttgaa gcgccgtggc
     2581 gtgcgggagt gtggggtgac gtaacagaag cgcatacatc ccaactgtct gcgggcgacc
     2641 tcgaccctgc cctcgaagat gctgttttcg gcgaggtcga tagcgtgggc ctggaccctg
     2701 ccgaatacgg tgctgcgcgc gacggtcagc ctcgcgtggg ccacgcgcca gtccggcgac
     2761 cctagcgcct cgcgctcggg gctggtcgcg tcaaggacgc tgtccctgat caggattcgg
     2821 accggctccg cctggacctc atcctgattt atctgaatgg agccgacgat gctgtgctcg
     2881 atgttgatgc acacgtacgg ggtgttgaac agttgcaggc tcggttcggc gggccggcgc
     2941 ggttcgcagt ctccgtgcag tccccagccg ggcacgaggg tggagtggcg gatggtaagt
     3001 ccggccagct ctccttccgc ctgcaccccc ggcccgtgac gagcaacccg tcaagcgtga
     3061 agcggctccc gcgcccgccc ttcacgctca gcgcgtcggg caggtccgtg tgccagtcaa
     3121 gcagccggat gaccggacgg acaccgtcgg ccgcgcggag ctgcagggtc tggtattccc
     3181 tgaggggtat gtagatctgc tccacgtaca ccccgctgtc ggtgatctcg atgacggcgt
     3241 gacgcggtct ctcttccctc cactggcgca cggccccgtt gatgcgcctg tgcgcttctc
     3301 caggaccaac gcggtagagc gcgaagctct tcgtcaactc ccgtgggtag gtttcttcca
     3361 gtaacagcct gttgagccgg gtcaggccgc gtccctcaag atcctggccg acgagccgca
     3421 tgatgttctc cggtaacgtt acgtgttcga agcgctgcgc ggcgtagagg ctctcgccct
     3481 ggagcagccg gttcagttcg tcgaccatgg ctttctgatg ctcttcagag ggggatccat
     3541 gctgttcttc gagcatctgc ttcccttcct cggagaaccg gtctctgagg taccgggata
     3601 gggggtcgcg gggctcttgc agcctgccta tcagggcggc gggatccttg agatcgtgtc
     3661 tatcgagcag caggtggtcg gggggctgtg ggagagacct ggcgtactcg ccgccgccga
     3721 tatccgccgt gaaaccgtaa tggtaggaga cccagaccct gtccggcacc tcgcgcggat
     3781 tgaaggcgat acgtccgagt tcgggatcta ccgcaacctg cccgcggcgt ggccggtaga
     3841 gccagtcggt caggtcggcg gcgacgatcc ggtccggcga gaccggcctg cgcgagtcct
     3901 gctccagtat ctccaggctc ttgccgggac cgtagtagtc ggtcttgtgg gcgtcgaacg
     3961 cccgtctcct gatgggggca ggcacgttca tcacttcggc tatgtgagta gggtcaggat
     4021 ctggctcagg acgggtatac agtggagcgt cgttgccgag gacgctgaag gtatagcagt
     4081 gtacccctgc gtcctccctg tagtaagccg gcgtctcggt taccggatag gacttcaacc
     4141 tccagacgaa gaggccgacg gtcgggatgt tgtagcgtcc gaggctacga tgcgagttga
     4201 tccgcctcac gtcggccgtg tgggccagct cgtcgaaagg gccatcgaga cggtccaggg
     4261 cgatctcctc gcgcacgtcc acggtacggc ctctcatcag ggtcacgtgg ttgatggcct
     4321 gggtcaggcc gagaaggttg tagaactcca cagcccgcgc gggccagccg gcgacgtcgc
     4381 tggcgagcaa ctccagcagc gccagcgtac ccttgcgccg cctgtagcgg atggtgttcg
     4441 ccacctcgcg gcgggggacg aggatgctgc cgcgtcccgc accctgggag gaacccgaat
     4501 ttcccgcctc gtacacgggc ctgtagccga tgatatcccc gagatactcg accacccagt
     4561 cctcgcaggt ctcgacgaac cagttttcgt agaggcggtc gatgtcctct tcgacgacgt
     4621 caacctgttc ggtgatgacc cgcagtagtg accggagcgg ctggccctgt tcctcgtcgc
     4681 gccggcggta gacggccgga agcagctcgt acaggcggtc ggcagagggg ctcatgctgt
     4741 tatctccttg agtatgagcg tgtcagggac caggggggtc aggaacgcga gttgggcagg
     4801 acgtatcgag atccctgagg agcccgccag atctaccctt acccgctggt acggctgctc
     4861 ctcgctctgc gtcagccgcg ttatctcggc ctcgatctcc gaagggagga ggacgcggcg
     4921 caggcctgtg gcgacgtcta ccgtcttctc ggggataccg ccgaacgtat ccacatcgac
     4981 gtaggcgacg ccggggacgt gctggatcgt gcttattacc tcgctcaaca gcgcgtcctg
     5041 gcccaggtca cgctgctcga agccgaaggt ctccaacagg gcagcccgga tctcgcgctc
     5101 gaccggctcc caaaggtaat ccggcagcaa cctcaccccg gcgctgacga ttagcaggag
     5161 aagctcgcgc acctcgaccc ggattggctg ttgcgggtcg ccgaaccgcc gcagcgcctg
     5221 gagcaggttc cggtagaggt ccgaggtctc ggcgatgggg atgtcgtcgg cgccagcgat
     5281 ggtgaggtga actagttgct gacgcccgtc tgagaggcgg gttgagctgg ctttgccgat
     5341 gcccgcgaag gcgtgggcga agtcctcgta gtcccttacc gagaccaggc ggtcaagtac
     5401 cttcacgccg agcggagcgt tgcggcgcgc tccttcgcgg gtttccctgt ccgcgccgcc
     5461 tgtggctggc tgggggttga tcacaccctt caacccgagc gggcgcgtcg cgagcaggct
     5521 gatctggccc tccgcgacgt tgcccgcctt gccgatgccc gtacggtaga cggccctgac
     5581 gttctccacg ccggtgggca gacgcgcccc gcgctgcccg tcaccgaaaa cgaccgtgac
     5641 ctcgtccaga tcgtcggtct gcgtgatgaa acggcgatcc gtcggctgca gcccggagag
     5701 gtgctcggcc tcgtgccagc ggatgtcgtt gacgtagact tcgagcgtgc tctccacacc
     5761 gctcggcgtg gtcgcggcca cgtgggtgag cggcgactga ttcaggctga acttctggaa
     5821 cgcttcgctg gcgtcgccgc tgccgagcac ctcgggacgc gtctcgccgt gcgtggcgtt
     5881 caccacgttg ccgtagatcc tgacggtttc ccgcttatag ggataggcca gatcgcctgc
     5941 gaggatgatg gtgctgtgga ccttctcgcc cgtcggctcc tccgcggata catggaagtc
     6001 gcctttgacc agtcgcagcc aggaccccgt cggtgcggag agattttcgc agtccccccc
     6061 gatgctggtg acgtaggaga tctcgtcgaa ggcagttatc ccgtccccgg cgtcgcggac
     6121 gtaccagttt atccgctggt cgcccgagtt cggtcccgtg tcggtccgcc tgacgtttat
     6181 gcaccagaag tccctctcat cgtcgggagg gacgatcccg acgacctcac cggtcgcgcc
     6241 gccaacgagg tgcgcgtagc ccgatactga gccgccggcg gcgcggcggg ccgatatcgt
     6301 caggtcgagc cctcccgggc ccgtgcttcc cctccccgtg agcctgagcg cggtggcctc
     6361 gccgacgcgc ggcaggttct cgtcagtgag gcttaccagc cgcaccccct gctcgactcc
     6421 ggcaagcatc acgagctcgc tcgcacgtac gccggggagg tcgtcgcgct ctccggtaac
     6481 gaagagccag cgtcccggct ccagg
//
LOCUS       DATAIR010000053         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_185979, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000053 DATAIR010000000
VERSION     DATAIR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185979"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..728
                     /gene="gmk"
                     /locus_tag="VHM69_01435"
     CDS             147..728
                     /gene="gmk"
                     /locus_tag="VHM69_01435"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2739080.1"
                     /translation="MRGRLIVVSGPSGAGKSTLITASLETVPELAYSVSATTREPRPG
                     EVDGKDYIFLSHEEFERWIEEDRFLEWAEYSGNLYGTPEKKVEELLDQGKSVILEIEL
                     QGARQVRMKRPDAVMVFVRAPSLEETRRRLTGRATETEEALESRLATAEGEVAASDEF
                     DYEIVNEDREQARKDMIELMKSIVTGGNDAARS"
     gene            712..1074
                     /gene="rpoZ"
                     /locus_tag="VHM69_01440"
     CDS             712..1074
                     /gene="rpoZ"
                     /locus_tag="VHM69_01440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX2739081.1"
                     /translation="MLHDLKIDDLLGKVDSRYGLAVASAKRARQINEYTSTLGLNDAL
                     GIPGPQVHTRSQHPLSIAIEELREGKLEISFRHSAEEVEEDVGAADGEDFISPPSGAG
                     VPQETDVDSGAPSDEGAA"
     gene            1081..>1702
                     /locus_tag="VHM69_01445"
     CDS             1081..>1702
                     /locus_tag="VHM69_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX2739082.1"
                     /translation="MILISVGASQGAIAVPGVAREIAGTGRRVEVILTHEARLFVGPA
                     AFDQATLVDEPSEPPEAVLFAPATSDTLARLARGLQGGPGDVSTTRTVVAPDLDPGTA
                     GHPAVRENLKLLREDGCRIIEGRDGGMAPAGEVVAGLLGGLGGPMSGRRLLITAGGTR
                     EPMDSVRFIGNRSSGKMGLAVAREALRLGAEVTVVAANMESVEPGTE"
BASE COUNT          340 a          501 c          582 g          279 t
ORIGIN      
        1 gccaaggtcg aggagatgct gctcgcggtc aagggcatgg gccgaataaa ggtaacgcgg
       61 gtcatgaggg aggcgggcat cagccgctcc aagacactcg tagggctcac gcatgggcag
      121 agggaccgtt tgatcagggc tttagggtgc gaggaaggct gatcgtcgtc tccggtcctt
      181 ccggagcagg aaagtcgaca ctgataaccg cctcgctgga gacggttccc gagctagctt
      241 attccgtatc ggcgacgacg cgagagccac gtcccggcga agtagacggt aaggactaca
      301 tctttctctc gcatgaggag tttgagcgct ggatcgagga ggatcgattt ctggagtggg
      361 ccgagtattc tggtaacctc tacgggaccc cggagaagaa ggtcgaggag cttctggacc
      421 agggaaagtc cgttatcctg gagatcgagc tccagggcgc gaggcaggta aggatgaagc
      481 gtccggatgc cgtgatggtc ttcgtacgcg ccccgtcgct ggaggagacc cgccgcaggc
      541 tcacaggaag ggcgaccgag accgaggaag ccctggagag ccggcttgcg accgccgaag
      601 gggaggttgc ggcaagtgac gagttcgatt acgagatcgt aaacgaagac agagagcagg
      661 cccgcaagga catgatcgag ttaatgaagt cgatagtaac aggaggcaac gatgctgcac
      721 gatcttaaga tagacgatct tctcggcaag gtggactcgc gctacggtct ggccgtagcc
      781 agcgccaagc gggcgcgcca gatcaacgag tacacctcca ccctgggctt gaacgacgcc
      841 ctcggcatcc ctggcccgca ggtccacacc cgttcccagc accccttgag catcgccatc
      901 gaggaattgc gcgagggcaa actggagatc tccttcagac actccgccga agaagttgag
      961 gaagacgtgg gcgcggcgga tggcgaagac ttcatcagcc ccccgagcgg cgccggtgtc
     1021 ccccaggaga ccgatgtgga ctccggagcc cccagtgacg aaggcgcagc ctaaaactcc
     1081 ttgatcctga tctccgtcgg agcgtctcag ggtgccattg cggtccccgg tgtggcccga
     1141 gagatcgccg gaacgggtcg tcgggtcgaa gtcatcctca ctcacgaagc tcgactgttc
     1201 gtcggacctg cggctttcga tcaggcgacg ctcgtggatg aaccctcgga accgccggag
     1261 gccgttctct tcgcccctgc gacatcggat accctggccc ggctcgcccg cggcctgcaa
     1321 ggtggccctg gcgacgtatc gacgacgcgc accgtcgtcg cccctgacct cgaccccggt
     1381 acggccggcc accctgccgt gcgggagaac ttgaagctcc tgcgggagga cggctgccgc
     1441 atcatcgaag ggcgtgacgg tggtatggcc ccagcgggag aggtcgtcgc cggattactc
     1501 ggcggcctcg gtgggccgat gagcgggcgg cggttgctca ttaccgcagg cggcaccagg
     1561 gagcccatgg acagcgtgcg ttttatcggg aaccgctcct cagggaagat gggcctcgcg
     1621 gtggcccgcg aggccctgcg gctaggcgct gaagtgaccg tcgtggctgc caacatggag
     1681 agtgtggaac cagggaccga ga
//
LOCUS       DATAIR010000054         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_324400, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000054 DATAIR010000000
VERSION     DATAIR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324400"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VHM69_01450"
     CDS             <1..1213
                     /locus_tag="VHM69_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739083.1"
                     /translation="GALALFFTLWATVAMVYRIEVVDLDPLRLVLLGTALEVSVFLFE
                     VPTGVLADTYGRRRSVITGCLIMGSGFALEGAIPTFAAVLAAQAVWGVGYTFISGALE
                     AWIADEAPEHDLGRVYLRGEQADYLGALVGIPASVLLGLVALNLPLLIGGGLTIALGL
                     ALFLAMPERNFRPSPREGRSSLQQVGATARDGARLVRGRPVLLMLIAVALFTGMSQEG
                     FDRLNAKHFLHGVGLPSIGGLDPVVWFGIMGAGGLILSYLAAGVVARNLDVGSPAVAA
                     RLLFALNFLTIAGMLAFALSGNFALALGAFWFATLVRTVAGPIYLTWINDGLDPGVRA
                     TVISMSCQSGALGEASAGPVIGVIGNAFGVRPALTITALILSPTLLLYGSAIRRGGVG
                     LKAGKLAGKGK"
     gene            1461..>1688
                     /locus_tag="VHM69_01455"
     CDS             1461..>1688
                     /locus_tag="VHM69_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739084.1"
                     /translation="MHESLRSKHNDRILPGLEALERESERLEGADPLEVIEWAVETYG
                     EGLALSASFGGGEGMALLDMISKVTDKVTVLT"
BASE COUNT          256 a          543 c          552 g          337 t
ORIGIN      
        1 aggggctttg gcgctcttct tcacgctgtg ggccaccgtc gcgatggtgt accggatcga
       61 ggtcgtggac ctggacccgc tgcggctggt gctccttggt acggcgctgg aggtctccgt
      121 gttcctcttc gaggttccca cgggcgtact ggcggacacc tacggccggc gccggtcggt
      181 tatcacgggc tgcttgataa tggggtccgg tttcgcgctc gagggagcca tcccgacttt
      241 cgctgctgtg ctcgctgccc aggccgtgtg gggggtcggg tacacgttta tcagcggggc
      301 gctggaagcc tggatcgcgg acgaagcccc ggaacacgac ctgggaaggg tgtacctgcg
      361 cggcgagcag gcagactatc tcggtgccct tgtggggatt ccagctagcg tgctcctcgg
      421 tctggtcgcc ctgaacctgc cgctcctgat cggcggagga cttaccatcg cgctcggcct
      481 cgcgctcttc ctcgcgatgc ccgaacgcaa cttccgcccc tcccctcgcg agggacgctc
      541 ctcgctgcag caggtgggag caacggcgcg cgacggcgcg cggctcgtcc gcggtaggcc
      601 cgtcctcctt atgctcatcg ccgtcgccct cttcaccggc atgtctcagg aaggcttcga
      661 ccgcctcaac gccaagcact tccttcacgg cgtgggcctt ccttcgatcg gcggccttga
      721 ccccgtggtg tggttcggca tcatgggcgc tggcgggctc atcctgagct acctcgcggc
      781 tggagtcgtg gcccgcaacc tcgacgttgg cagcccggcc gtcgccgcga ggctgctgtt
      841 cgcgctcaac ttcctcacca tagccggcat gctcgccttc gcgctgtccg gcaacttcgc
      901 gctcgcgctc ggggcgttct ggttcgccac cctggtacgc acggtcgcgg ggccgatcta
      961 ccttacctgg atcaacgacg ggctggaccc gggggtgcgg gcgaccgtga tctcgatgag
     1021 ttgccagtcg ggcgccctcg gcgaggcttc agcaggacct gtgatcgggg tcataggcaa
     1081 cgcgttcggg gtaaggcccg ccctcaccat aaccgccctc atcctctctc cgaccctcct
     1141 gctctacgga agcgccatcc ggcgcggcgg cgtcgggcta aaggccggca aactggccgg
     1201 aaagggaaaa taacaagccg tggagggcgc cttctcgccg gaaatgagca gaatatgttc
     1261 aattccgacg aaaattatcg gatttttgtt gacgtatcgg tctttggcgc ttagtatccc
     1321 acgcatggag ttgctagaca cgcagggaca cgcggcgggg cgaatgtcat tggcctctcg
     1381 cacgggcgca cgatgtagcg tgcgcgcctg atctcaaccc ctgactttca gttacacgat
     1441 tacattggag gatcgccgcg atgcacgaat ccctgcgttc gaagcataac gacagaatct
     1501 tacctggcct cgaggcgctc gaaagggagt ccgagcgtct tgagggtgcc gatccgctgg
     1561 aggtaataga gtgggcggtg gagacctacg gtgagggcct ggcgctctcg gcctcgttcg
     1621 gcgggggcga ggggatggcg cttctggaca tgatctccaa ggtcaccgac aaggtcaccg
     1681 tcctcacc
//
LOCUS       DATAIR010000055         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_232122, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000055 DATAIR010000000
VERSION     DATAIR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232122"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VHM69_01460"
     CDS             <1..415
                     /locus_tag="VHM69_01460"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2739085.1"
                     /translation="ALGTAAVLVIGLLSWNVLLQNQVQDLRGEVKDAQVQTVELQGSW
                     AEQGAHAEVAAINENRVILVAEDMPSVPDNKTCQIWVIHEDEPKPSGLFQPDADMTAT
                     AITTPIKKSDVIAVTVEPAGGSTKPTSDPVLAAKL"
     gene            528..731
                     /locus_tag="VHM69_01465"
     CDS             528..731
                     /locus_tag="VHM69_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2739086.1"
                     /translation="MTVARVTELSAVSETSFDDAIKQGVSRATSTLRGIEGAWIKDMN
                     VLIEDGNITGYKVNIEVTFVLED"
     gene            1087..1545
                     /locus_tag="VHM69_01470"
     CDS             1087..1545
                     /locus_tag="VHM69_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2739087.1"
                     /translation="MHTNPNNREIRDLLQRSRTVAVVGLSDNPYRTSHVIAGALQRFG
                     FEVFPVNPNLKGRVLGEEPYSSLEDLTIAVDIVNVFRSSEKVMPVAEDAVAMGAKVLW
                     MQSGVVNEEAAAYAEEHGLTVVMDRCIKVDYATLVGQAGSHAVPPRREGE"
     gene            1568..2071
                     /locus_tag="VHM69_01475"
     CDS             1568..2071
                     /locus_tag="VHM69_01475"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX2739088.1"
                     /translation="MSAPDGRRRLGEAPPLFMFFYGTLKRGQSNYDKYCSGALRVEGA
                     TVRGEIYDLPFGYPALVVPHESIHASGTGDPTVDAEKQRRLDRAQVAFPEGPRVSGEL
                     FVFDDPGSRLPLIDRLEGFDPADDASHYRRVLLPTETNEGSRILAWAYVVDKTSGTYL
                     PGGSWPP"
     gene            complement(2122..2880)
                     /locus_tag="VHM69_01480"
     CDS             complement(2122..2880)
                     /locus_tag="VHM69_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2739089.1"
                     /translation="MFTIGHGAGTHANTNRRLIEEENVASEDRLRELEEKYEDYKVYD
                     NQGQKIGKVDDLFIDESDREEYIGVKMGFFGMKSTLIPMEIVRVNENERSIEVSDSKD
                     HVKDAPSFDDDEDITSEYEGRIRDHFGLESLSTSGSNYGAYADSNADSHDYDDHSSAD
                     TEHEERAEGSDSREVPEYGGAGESSDDREESTEAGSGIAGTSSTDEEATREVPTTRQT
                     EETETYKEGGRTKVRRRVVREEIFEEGDDQTTRS"
BASE COUNT          578 a          919 c          911 g          601 t
ORIGIN      
        1 cgcgcttggc actgcggcgg tgctcgtgat cgggctcctc tcctggaacg tgctgctgca
       61 gaaccaggta caggaccttc gaggcgaggt aaaggacgca caggtccaga cagtcgagct
      121 tcagggatcg tgggcggagc agggtgctca cgctgaagta gccgccatca atgagaaccg
      181 ggtgatcctg gtcgccgagg acatgccctc cgtgcctgat aataagacgt gccagatctg
      241 ggtgattcac gaagacgaac ccaagcccag cggcctcttc caacccgacg cggacatgac
      301 cgcgacggcg atcaccactc ctataaagaa gtccgacgtg atcgcggtga ccgtcgagcc
      361 ggccggaggc tcgacgaagc cgacgagcga tcccgtcctt gccgccaaac tctagcgaag
      421 cgcgtctcag gcccttcgcg cccgccccaa agtgtgttta tgatcagcgt ccgggggtag
      481 taattatcta gggaaagtag cgaacggatc aaccggaagg agtgtctatg actgtagcca
      541 gagtaacgga gttgagcgcc gtctcggaaa cgagcttcga cgacgccatc aagcaaggtg
      601 tgagccgggc tacttccacg cttcgcggca tcgagggggc ctggatcaaa gacatgaacg
      661 tcctgatcga ggacggtaac atcacaggct acaaggtgaa tatagaggtt accttcgtcc
      721 tggaagatta gcgacgaagg ctccacgcaa cgctctgccc cggcccaccg gccggggctt
      781 ttttgttccc ttctcgactg aagattgcac ggtacggcac agggcaagag acttccgtgg
      841 gaagcaggtt acagtagtgc cggcgattag agacgccttt caccacgatc tcctcccgtg
      901 gccttgcgct taaggagcag ggcgcacgcc tgcccggtag ttagccggga ctggcacgat
      961 cggccagccg ccctcgaagg cagtaaggcg gacttgatgt acgaggcgat gcgtaaagtg
     1021 cacgcgctcg tgccaatagg cgtcgagatc gggtaacatc tgaaccgtac atttcggagg
     1081 gagcacatgc acacgaaccc caataatcga gagataagag acctgctaca gcggtcgcgc
     1141 acggtggcgg tcgtcgggct ctcggacaat ccgtaccgaa cgagccacgt gatcgcgggc
     1201 gctcttcagc gcttcggctt cgaggtcttc ccggtcaatc ccaacctcaa gggtcgggtc
     1261 ctgggtgaag agccgtactc gtcactcgaa gacctcacca tagctgtgga tatcgtgaac
     1321 gtattccgca gcagcgagaa ggtcatgccc gtggcggagg acgcggtggc gatgggcgct
     1381 aaggtactct ggatgcagtc cggcgtcgtt aacgaggagg cggccgccta cgccgaagag
     1441 cacggcctca ccgtcgtaat ggaccgctgc atcaaagtcg actacgcgac tctggtcggg
     1501 caagcaggct cacacgcagt cccgccgcgc cgggaaggcg aatagccgaa gggcagatag
     1561 tagacgcatg tcggcacccg atggcaggcg ccgcctgggc gaggctccgc cgctattcat
     1621 gttcttttac ggaacgctca agcgtggcca gagcaactac gacaagtact gcagcggtgc
     1681 gctgcgcgtt gaaggtgcga cggtgcgggg agaaatctac gacctcccct tcggttatcc
     1741 ggctctggtc gtgccacacg aatccatcca cgcctcaggt accggagatc cgaccgtgga
     1801 cgcagaaaag cagaggcgcc tggaccgcgc gcaagtagcc tttccagagg gtccgcgcgt
     1861 ctccggagag ctcttcgtct tcgacgaccc tgggtcgcgc cttccactaa tcgaccgcct
     1921 ggaaggcttt gatcccgccg acgatgcgag ccactaccgc cgcgttttgc tcccgaccga
     1981 gaccaacgaa ggttcacgca tcctggcatg ggcatacgtg gtcgataaaa cttccggaac
     2041 ctacctacca ggaggaagct ggcccccgta gccgacgccc ggtcaagctt ctagctctcc
     2101 cctgtcgacc agctattcgc ttcagctgcg cgttgtctgg tcgtcgcctt cctcgaagat
     2161 ctcctctcgc accacgcgtc ggcggacctt cgtgcgtccg ccctccttgt aggtctccgt
     2221 ctcctcggtc tgacgcgtgg tcggcacctc cctcgtagcc tcctcatcag tggagctggt
     2281 gccagcgatt ccggatcctg cctcggtgga ttcttccctg tcgtctgagg actcacccgc
     2341 gcccccgtac tcgggcactt ccctggagtc actgccttcc gcgcgttcct cgtgctctgt
     2401 atcagctgag gaatgatcgt cgtagtcatg tgagtcggcg ttcgagtccg cgtacgcgcc
     2461 gtagttactg ccggaggtac tcagggattc gaggccgaag tggtcgcgga tgcggccctc
     2521 gtactccgag gtaatgtcct cgtcgtcgtc gaagctcggg gcgtctttca cgtggtcctt
     2581 cgagtcggag acctcgatgc tccgttcgtt ttcgttcacg cggacgatct ccatcgggat
     2641 cagggtggac ttcatcccga agaaccccat cttgaccccg atgtactcct cgcggtctga
     2701 ctcatcgatg aagaggtcgt cgaccttccc gatcttctgc ccctggttgt cgtagacctt
     2761 gtaatcctcg tacttctcct caagctccct tagcctgtct tcgctcgcca cgttctcctc
     2821 ctcgatgagc ctgcggttgg tattagcgtg ggtacccgcg ccgtggccga tggtaaacat
     2881 gtaaccgggt agcgcttgct aaactacctg aagggtggtc tgtgtggaag gtgggagcgt
     2941 gggatccatt gaatcgcaag atgcagggat tctctctgta gacgtagggt cctcggcggt
     3001 gagagccgg
//
LOCUS       DATAIR010000056         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_418478, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000056 DATAIR010000000
VERSION     DATAIR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418478"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VHM69_01485"
     CDS             <1..967
                     /locus_tag="VHM69_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2739090.1"
                     /translation="WRRAAVRYPALPAMKRRLSGITLGITRFVSSVRGRSGAADTGAL
                     GLDPANITWIFCVGRSGSTWLSNVMGELGGHKIWREPKIGQLFGEFYASTPKQKRDSA
                     NFIMGAPTRKAWISSIRNFVLVGARHAFPLLRPDQQLVIKEPGGGVGAPLLMEALPES
                     RMILLVRDPRDIVSSWLDAAGEGGWKRQNRRDASNRESLAEKRPDAVVRKLARRYLNN
                     LQMAKQAYDDHRGPKTLVLYERLRVDTLGVMKGMYLDLGIEIDEAQLARAVEKHAWEN
                     IPEDLKGEGKFFRKASPGGWSEDLTPKQAEMVERITAPVLDEFYA"
     gene            complement(1129..>1551)
                     /locus_tag="VHM69_01490"
     CDS             complement(1129..>1551)
                     /locus_tag="VHM69_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739091.1"
                     /translation="QFPSVQTGLSRSETQLLDAILHGKRILREAFVSSQDREERLFLG
                     DSVFAAYLEDLSATGEPLVLYSDGTRIVAPRDPASLNGFWDNEAMVTQTGRAVLEGNR
                     DRLGLYPIDRWLGGVHLSGTEARWRWDESTSQLRRGAA"
BASE COUNT          309 a          431 c          523 g          288 t
ORIGIN      
        1 gtggcggcgc gccgcggtcc ggtatcctgc gcttccagcc atgaagcgca ggctgtctgg
       61 catcaccctc gggataaccc gtttcgtgtc ctcggtacgc gggagatctg gcgccgcgga
      121 cactggagcg ttgggcctcg atcccgccaa catcacctgg atattctgcg tcggcaggag
      181 tgggagcacg tggctgagca acgtgatggg ggagcttggt gggcacaaga tctggaggga
      241 accgaagatc ggacagctct tcggcgagtt ctacgcaagc accccgaaac agaagaggga
      301 ctcagcgaac ttcatcatgg gagccccgac aaggaaagcc tggatctcct ctataaggaa
      361 cttcgtgctg gtgggcgcac gacacgcctt ccccctgctt cgcccggacc aacagctggt
      421 tatcaaggag ccaggtggcg gcgtcggggc gcctctgctg atggaagctc tgccggagag
      481 ccggatgatc ctgctcgttc gggacccgag ggacatagtg tcttcatggc tcgacgcagc
      541 gggcgagggt ggatggaaga ggcagaaccg gcgcgacgcc tcgaaccgcg aatcacttgc
      601 ggagaagcgg cctgacgccg tagtcaggaa actggccagg aggtatctga acaatctgca
      661 gatggccaag caagcatatg acgatcacag gggccccaag acgctggttc tttacgagcg
      721 cctcagggtc gacactcttg gcgttatgaa gggcatgtac ttggatctgg ggatagagat
      781 cgacgaggcg caactcgcgc gggccgtgga gaaacatgcc tgggagaaca tacccgagga
      841 cctgaaagga gaaggcaagt tcttcaggaa ggcttcgccc gggggatgga gtgaggatct
      901 gacacccaag caggcggaga tggttgagag gataacagcc cccgtcctcg acgagttcta
      961 cgcctgaaga cgggcttcag gcatcaggtt tcaggcttca gggattgttg cgggaaggga
     1021 tctcgttcgc tgatgggggg gcctgaggcg atggatcatt ctccaccaac acggctatga
     1081 gtagcctcgg cagggcagtt tgccgcggtt atttccagta tggcggcgct aggccgcgcc
     1141 gcgtctgagc tgactggtgg actcgtccca gcgccaccgt gcttcggtgc cgctcaggtg
     1201 gactcctccc aaccagcggt caatggggta cagtccgagt ctgtctcgat ttccttccag
     1261 taccgctcgc ccggtctgcg ttaccatggc ttcgttgtcc cagaatccat tgagggatgc
     1321 gggatcacgc ggggccacga tccgtgttcc gtccgagtag aggacgagag gttcgccggt
     1381 cgcgctcaag tcctcgaggt acgccgcgaa gaccgagtcg ccgagaaaga ggcgttcttc
     1441 tctgtcctgg gatgagacga acgcttcgcg gaggatcctc ttgccgtgca ggatcgcgtc
     1501 aaggagctgc gtctcggaac gcgagaggcc ggtctgtacc gacgggaact g
//
LOCUS       DATAIR010000057         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_3222, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000057 DATAIR010000000
VERSION     DATAIR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1207)
                     /locus_tag="VHM69_01495"
     CDS             complement(5..1207)
                     /locus_tag="VHM69_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739092.1"
                     /translation="MRPFEGAELEAELEDKVSLLRVQAYPDFPEVREIEHYAALYRWY
                     QTHPLADEMHRWVAMTEEGEVVGHLAGFPQFYRIDGQRVVAHTPGDYMVLPQYGFQAI
                     MLMRSFFRATENCVVSDMVPAVISIETRLGAEVAGELHYAAKLMNVSRLPMPSVPAPL
                     RRLLKLPEQFAPARGFTGRPGAESQEVEEHVAPPAPRPRAPIPRPVKWLLNRGLSTVD
                     DALGKRFGGTLEAEVLDGFDDTFDDLFERVATVVPCVAEKDAAFLRWRYGPGSPQAPV
                     TVLGVRFGGRLLGYAVLKVAIHGQDGYILDLTVLPGRQDVVRALLRETVRYFRKAGVQ
                     IIRYRFLESPSSPDQTDLLRLGFFYRRGRRHNLLVKFKDKNLHETARDLVNWSHSFGD
                     GEAGFWVR"
     gene            1627..3069
                     /locus_tag="VHM69_01500"
     CDS             1627..3069
                     /locus_tag="VHM69_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX2739093.1"
                     /translation="MKEISKEGGPRATRRSTREANVGMRKYRKSRYARRRGAALAALI
                     LGILLVGVAAVDYLAGGDDEINRGVRIGSVDVGGMTKDEASKAIERDASTTFEKISFD
                     AGKQSYSLSGDELGVKVDTDSAVDAAYSVGRRGNALQRLSDASRSYLGGVRVDLAAGY
                     EEDEARSALGKLADEFNQKPQNASFSVTDDGKVVVKKAHDGRVLDQEETLSNLEGALK
                     NMSGRVAIAEGPAPKPEVSTSEVDKYKPEKVIGEYKTDFLWDSNPNRKYNMKLAAGAV
                     NNTVLAPGEVFSFNKLASSLDYKEAKTFSDGGVGIANGGGLCQVSSTLYMAANYAGLR
                     IIERHPHYAVLPYIKPGFDATVWFGENGWGAQDMQFKNTSDGYIVIREWVDEKGFLNA
                     QILGQPTGMKVEMRTEKIFEDTTRGIKWTTYKKVTRDGKVIRDGFLHTYRYSYNPPVP
                     DDAPHYDTNAPRVAGWSDPGNTTGWAEVPE"
     gene            3118..4599
                     /locus_tag="VHM69_01505"
     CDS             3118..4599
                     /locus_tag="VHM69_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2739094.1"
                     /translation="MNLFPRRKLFAAKRRRQLLQKFGALAVLVVLLAGAIYVAGRTSD
                     GAPSASQEDGAPSHETPAQRGEAKSEEAPAKKPDKAAPERTDPEQPTPEAAAYAVLAP
                     ELPGTTPESIRSVYRSKMDKSWASVRVEPQGEGKPFVVFTHWDGKSWRAEKSVRADEP
                     DYAKNDVVPLAGVPDDLIQYLYPENVFAAKVPEPTIEKIDRSGLPQVESAEFSTTEPV
                     MDGVPESDRERIKKVLAQTRQEIEGYNGVAGLYVWDLNEDFGYGIRPDEQFFTASIIK
                     VPVMVAVYRKVDEGKLTFSQQIEIKEEDWAAGAGWLQWEKAGTKQTVGDLLLLMMTQS
                     DNVATNALVRTVGGAGHVNEVARSMGAEDTLLYQKVSSERGAVPALDNRSTPRDMAVM
                     LQKIAEGKAASEKSCEYMIELMYLDELDWWLDAGLPAGVEAANKAGWLYKVYDEVGIV
                     QSGGHPYVITILSKHGTANVYEGQDLIRNLSSAAWEAQSQQGN"
BASE COUNT          959 a         1474 c         1530 g          751 t
ORIGIN      
        1 cctcctatct gacccagaat cccgcttcgc cgtcgccgaa agagtgcgac cagttgacga
       61 ggtcgcgggc cgtttcgtgc aggtttttat ctttgaactt gaccagcaga ttgtggcgtc
      121 ttcccctgcg gtagaagaag ccaagccgca gcaggtcggt ctgatcgggc gaagagggcg
      181 attccaggaa cctgtacctg attatctgca cccccgcctt acggaagtaa cgcaccgtct
      241 cacgcagcag cgcccgtacg acgtcctggc gtcccggaag caccgtcagg tcgagtatgt
      301 agccgtcctg gccatgaatg gcgaccttga gtacggcgta tccgagcagc ctccccccga
      361 acctgacccc gagcacggtc accggtgcct ggggagagcc cggtccgtaa cgccagcgca
      421 ggaacgctgc gtccttctcc gccacgcagg gcacgacggt ggcgaccctc tcgaacaggt
      481 cgtcgaaagt atcgtcgaac ccatcaagca cctccgcctc cagcgtgccg ccgaagcgct
      541 tgccaagggc gtcatcgacc gtgctgaggc cgcgattcag caaccacttc acggggcgag
      601 ggatcggggc gcgcgggcgt ggtgccggcg gtgcgacgtg ctcctccacc tcttgcgact
      661 cggctccggg cctgccggtg aacccgcggg caggcgcgaa ctgctcagga agtttgagga
      721 gcctcctcag cggcgctggt accgatggca tcggaagccg cgagacgttc atcaacttcg
      781 ccgcgtaatg cagctcacca gccacctcgg cgccaagccg cgtctcgatg ctgatcaccg
      841 cgggcaccat gtcgctgaca acgcagttct cggtggcgcg gaaaaaggag cgcatcaaca
      901 tgatggcctg gaaaccgtac tgggggagca ccatgtaatc ccccggcgtg tgggcgacga
      961 cccgctggcc gtcgatcctg tagaactgcg ggaacccggc caggtggccg acgacctctc
     1021 cctcttccgt catcgcgacc cagcggtgca tctcgtcggc gagcgggtgg gtctggtacc
     1081 agcggtagag agcggcgtaa tgctctatct cgcgaacttc ggggaagtcc ggatacgcct
     1141 gaacgcgcaa tagcgagacc ttgtcttcca gctccgcttc cagttccgca ccctcaaacg
     1201 gcctcaccgc cgccttcatc gaatgtaccc cgatcccgcg gcccgtgtgg acaaggatgc
     1261 cggcctcccc ggcaagtgcc gtccctgttt tgcttgcttt gattctgatt cgtggcgcaa
     1321 gaaaccttcc actctgtagg gttttgtacg gtgcaagtcc aaagagcaaa gtgtaatgga
     1381 gccattcgta aaagccaagc gaggtgtctc ccggaatcgg ggccatcgcc tccggttacc
     1441 agggcgttcc agccgggttc ccgtactgga gatgggcacg tggcggtaga gtttaggagc
     1501 aattccgaga cattttgtcc ggagaggttg aaggttcggc cacttgcctc gataattagc
     1561 gcctgcttgc caccgtgacc tcgcaaaggc ggtggcgcta atcggtatcg caggaggggc
     1621 gaatacttga aagagataag caaggagggt ggaccacgcg ccacccggcg gtccacccgg
     1681 gaggcgaacg tggggatgcg taagtaccgc aagtcacgct acgcccgcag gcgcggggcc
     1741 gcgctcgccg ccctgatcct gggcatcctg ctcgtcgggg tcgccgccgt cgactacctg
     1801 gccgggggtg acgatgagat caaccgcggc gtccgcatcg gttccgtcga cgtcggcggt
     1861 atgaccaaag acgaggccag caaggccata gagcgcgacg cctccacgac cttcgagaag
     1921 atctccttcg atgcgggtaa gcagagctac tcgcttagcg gtgacgaact cggcgtgaag
     1981 gtggacaccg actcggcggt ggacgcggca tactccgtcg ggcggagggg gaacgcgctg
     2041 cagcgtctct ccgacgcctc gcgctcgtac ctgggagggg tgcgggtgga cctcgccgcc
     2101 ggatacgagg aggacgaggc caggagcgcg ctgggcaagc tcgccgacga gttcaaccag
     2161 aagccccaga acgcctcctt cagcgtcacc gacgacggga aggtggtagt caagaaagcg
     2221 cacgacgggc gagtcctgga tcaggaggag accctgtcga accttgaagg ggcgctgaag
     2281 aacatgagcg ggcgggtcgc catcgccgaa ggtcccgctc cgaagcccga ggtctccacg
     2341 tccgaggtcg acaagtacaa gccagagaag gtcatcgggg agtacaagac cgacttcctg
     2401 tgggactcca acccgaaccg gaaatacaac atgaagctgg ccgccggggc cgtcaacaac
     2461 accgtcctgg cgcctggcga ggtgttctcc ttcaacaagc tggcctcgtc gctcgactac
     2521 aaggaggcga agaccttctc cgacggtggc gtcggcatcg caaacggcgg tgggctctgc
     2581 caggtgtcct ccacgctcta catggcggcc aattacgccg ggctccggat catcgagcgt
     2641 cacccgcact acgccgtgct cccctacatc aagccaggct tcgacgcgac ggtgtggttc
     2701 ggggagaacg gatggggcgc ccaggacatg cagttcaaga acacctccga cgggtacatc
     2761 gtcatccgcg agtgggtcga tgaaaagggc ttcctaaacg cccagatcct cggccagccc
     2821 acgggcatga aggtcgagat gcgtacggag aagatcttcg aggacaccac gcgtgggatc
     2881 aagtggacca cctacaagaa ggtgaccagg gacggcaagg tgatccggga cggcttcctg
     2941 cacacgtacc gctacagcta taacccgccc gtccctgatg acgcaccgca ctacgacacc
     3001 aacgccccgc gggtcgcggg atggtcggac cccggcaaca ccaccggctg ggcggaagta
     3061 ccagagtaac gagatggccg agggaatgca caggcttcgt cgcgcgcggg ggaccttttg
     3121 aatctcttcc ctcgccggaa attgttcgcc gccaagcgcc gtcgccagtt gctccagaag
     3181 ttcggtgccc ttgccgtgct cgttgtgctc ctggccggag ctatctatgt cgcgggccgg
     3241 acctcggacg gggcaccaag cgcttcgcag gaggacggcg ctccttcaca cgagacgcca
     3301 gcgcagcgcg gggaggcgaa gtctgaagag gctcccgcga agaaaccgga caaagcggcg
     3361 cccgagagaa ccgaccccga gcagcccacg cccgaggcgg cggcatacgc cgtcctcgcc
     3421 cccgagttgc cggggacgac ccccgagagc ataaggagcg tataccgcag caagatggac
     3481 aaatcctggg cctccgtgcg cgtcgagcct cagggcgagg gaaagccctt cgtggtcttt
     3541 acccattggg atggcaaatc gtggagggct gaaaagtccg tgcgcgcgga cgagcccgac
     3601 tacgccaaga acgacgtggt cccgcttgca ggcgtgcctg acgacctcat ccagtacctc
     3661 tacccggaga acgtgttcgc tgccaaagtt cccgagccga ccatcgagaa gatcgaccgt
     3721 tcaggcctgc cgcaggtcga atcggcagag ttctctacga cggagccagt gatggatggg
     3781 gtgccagagt ccgaccgcga gcggatcaag aaggtccttg cacagacgcg gcaggagatc
     3841 gagggctaca acggggtagc cggtctctac gtgtgggacc tgaacgagga cttcgggtat
     3901 gggatcaggc ccgacgagca gttcttcacg gcctcgatca taaaggtgcc cgtgatggtc
     3961 gccgtctacc gcaaggtgga cgagggcaag ctcacctttt cgcagcagat agagatcaag
     4021 gaagaagact gggccgccgg agccggctgg ctgcagtggg agaaggctgg gacgaaacag
     4081 acggtgggag acctcctgct gcttatgatg acgcagtctg acaacgtcgc caccaacgcc
     4141 ctcgtgcgga cggttggcgg ggcaggccac gtcaacgagg tcgcccgctc tatgggtgcc
     4201 gaggacacgc tgctctacca gaaagtgagc agcgagaggg gagccgtccc tgccctcgac
     4261 aacaggagta cgccacgcga catggcggta atgctgcaga agattgcgga aggcaaggcc
     4321 gcgagcgaga agagctgcga gtacatgatc gagctcatgt acctcgacga gctcgactgg
     4381 tggctcgacg cgggactgcc ggcaggcgtg gaggccgcga acaaggcagg ctggctctac
     4441 aaggtctacg acgaagttgg gatagtccag tccggcggcc acccttacgt gataacgatc
     4501 ctgagcaagc acggcacggc caacgtgtac gaggggcagg acctgatcag gaacctttcg
     4561 agcgccgcct gggaggccca gagccagcaa gggaactgag ggaagtctcc tagcccgcgg
     4621 acttcccggc gcgccgccgc aacagggcga tgacggactt caactcctcc acccccagtc
     4681 ggtacgccac cccgaggtag acggcgagcg atgc
//
LOCUS       DATAIR010000058         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_47609, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000058 DATAIR010000000
VERSION     DATAIR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47609"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..934)
                     /locus_tag="VHM69_01510"
     CDS             complement(164..934)
                     /locus_tag="VHM69_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739095.1"
                     /translation="MPEIPIEVAYPVSDELHLHLALGACRLKAGPGEGGAWISGTCKD
                     PTGKRAPTVVEEGGRVRITESEPSFERIPAVFGGIPRYELRFGKSRPFALTVETGASE
                     FEMDLGGIPVKALTVRQGAGKFELDFSASNPHPMEVLEVSSGAAGIELENLANANFSE
                     MRLSGGAASYELDFGGVLSRDAEAKIETGLSGVEIEVPASTAAKIVAETTLGSVDVGD
                     GFTKREGAFLTERALAGGEPLLTIRAGVRLGSLQLRAT"
     gene            complement(1013..1417)
                     /locus_tag="VHM69_01515"
     CDS             complement(1013..1417)
                     /locus_tag="VHM69_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739096.1"
                     /translation="MVTKAVARIMLVVLEVLMGMAAVGGGIGLILTNGLGMPVESLDG
                     SPFGSYTIPGLVLIFVGIINLVSAATILRRHRWGVPLSGLVGLMWMGWFVVQVAVVGL
                     ISWQQPFYFVVGLLILLLAAPSLIGQYRMRSA"
     gene            complement(1561..1761)
                     /locus_tag="VHM69_01520"
     CDS             complement(1561..1761)
                     /locus_tag="VHM69_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739097.1"
                     /translation="MWWADLLWGFWNGLTAWIVLIVHVFGGWEDFPFYNAARSGNWYD
                     FGFLLGAGSPILGAFGGRSRRG"
     gene            complement(1858..2277)
                     /locus_tag="VHM69_01525"
     CDS             complement(1858..2277)
                     /locus_tag="VHM69_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739098.1"
                     /translation="MARVVNRIGASVYARGVASNYLVTLEVPGRQSGRTISLPLVMAV
                     VAGERYLVSMLGDDVNWVRNVREAGGDVTLRHGRREEVRLEEIPPDRRAPVLKAYLKR
                     APGARAHLPVDKDAPLEEFERVSPRFPVFRVVPKQTA"
     gene            complement(2386..>2845)
                     /locus_tag="VHM69_01530"
     CDS             complement(2386..>2845)
                     /locus_tag="VHM69_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739099.1"
                     /translation="LSLDLLFTLRHRPDRMSVRRVAAIIYAAVSAGVVAFQVALAAGA
                     PWGAYAMGGAFPGQFPPTLRIAALVQAALFAGMAAVVMSRAGLVLDGWSRASRWLVWV
                     VVAIAAVSLVLNLITPSAGERAIWAPVALLLLASSAVVAIEGVARGERRR"
BASE COUNT          565 a          967 c          813 g          500 t
ORIGIN      
        1 gcttcctgcc tgatcagcat gaaccatatc gatagtcaaa gtaggccaaa cgcagcccag
       61 ccgacctctg tgggtcagcg gcatcttctg ccggggcgcg tccccggtgc gatggaaggg
      121 cgagggcctc tgcaatgcaa gatctccgaa gttccggttg cgctcacgta gcccttagct
      181 ggagggagcc gagtctgaca ccggcgcgga tcgtcaggag cggctcgcct ccggctaacg
      241 cgcgctccgt caagaacgct ccctccctct tcgtgaaccc gtccccgacg tccacgctac
      301 caagcgtcgt ctcggccacg atctttgccg ccgtggaggc ggggacttcg atctccaccc
      361 cgctcagccc ggtctcgatc ttggcctcgg cgtcccgcga gagcacaccg ccgaagtcga
      421 gctcgtagct ggcggctcct cctgagagcc gcatctccga gaagttggcg ttggcgaggt
      481 tctccagctc gataccggct gccccgcttg agacttcgag cacctccatg ggatgtggat
      541 tggaggcgga gaagtcgagc tcgaacttgc ctgcgccctg cctgaccgtc aacgccttca
      601 cggggatgcc acccagatcc atctcgaact cgctcgcgcc cgtctctacg gtcagtgcaa
      661 atggtcgcga cttgccgaag cggagttcgt agcgtgggat gccgccgaag acggcaggga
      721 tgcgctcgaa ggagggctcg gactcggtga tcctgacccg tcccccttcc tcgaccacgg
      781 taggcgcccg tttgccggtt ggatctttgc acgtgccgct gatccaggct cctccctcgc
      841 ccggtcctgc tttcaagcgg caggccccga gcgcgagatg caggtgcagc tcgtccgaga
      901 cggggtaggc gacttctatc ggtatctctg gcatgagagc ctcctttcgt gaccgtctat
      961 ccttcgtgtg atgctggttg accaccgtgg gaacgggtgc tcgaagtaac gtctaggcac
     1021 ttcgcatccg atactgtccg atcagcgacg gtgccgcaag caagagtatc agcaggccga
     1081 ctacgaagta gaagggttgc tgccagctta tgaggccgac gacggcaacc tgcaccacga
     1141 accaccccat ccacatcaag cccaccaatc cagataaggg aacaccccac cggtgacgac
     1201 gcaaaatggt ggccgcgctc accaggttga tgatacccac aaagatcagc actagccctg
     1261 ggatggtgta gctgccaaag ggagagccgt ccagcgactc gaccggcatc ccgagcccgt
     1321 tggtcaggat gaggccgata ccgcctccca cagcggccat acccatcaag acctctagca
     1381 cgacgagcat gatacgcgcc actgctttgg tcaccatcac cttccttctt ctttccgccc
     1441 gggattttac aagcacaact ctaggagacg aagtagttcc atgcctcatc agaatagacc
     1501 aacgttatga gcaatctttg tcaaagaccg agggccgaac aacgcgacca gagcgtatct
     1561 ttaccctcgt cggctcctcc caccgaaggc gcccagaatg ggggaccccg cgccgaggag
     1621 aaatccgaaa tcgtaccagt ttccgcttct cgcggcgttg tagaagggga aatcctccca
     1681 gccgccgaac acatggacga taaggactat ccaggccgtc aggccgttcc agaatcccca
     1741 caacaagtct gcccaccaca tgatggttct ctcctttcag tctcgctgcc cggactcacc
     1801 ccaggatgtc tcatatggaa cggatcgcgg ctgatcgtca cgcgcacctc cggcatgtca
     1861 cgccgtctgc ttcggtacga ccctgaacac ggggaaccgg ggtgacactc gctcgaactc
     1921 ttcaagcggc gcgtccttgt cgacaggcag atgggctctg gcaccaggcg ccctctttag
     1981 ataagccttc agcacgggtg cgcggcgatc cggcgggatc tcttccaggc gcacctcctc
     2041 gcgacggcca tgacgcaggg tgacgtcgcc tcccgcctcc ctcacgttcc gcacccagtt
     2101 cacgtcatct cctagcatgg agacgagata gcgctcaccc gcaacgacgg ccatgacgag
     2161 cggcaggctg atggtccgac cagactgccg gcccggcacc tcgagtgtga ccaggtaatt
     2221 cgaagcaacc ccgcgcgcat agactgaggc cccgattcgg ttgacaacgc gagccacccg
     2281 attggcacgt ccaccacggt ataaccatct cgccagactc atcaccgtcc tcctctgttt
     2341 ggtgggaggc gaaggatcat ccgactcatg ccggcacctt ccatctcatc gtctcctttc
     2401 acctctggcg accccctcta tggctacgac cgcgctgctt gccagcagca gcaaagccac
     2461 cggcgcccat atggcgcgct cacccgcgct gggtgtgatc aggttgagca cgaggctcac
     2521 ggccgcgatc gcgacgacga cccaaacgag ccagcgcgac gcgcgagacc atccgtcgag
     2581 gacgagcccg gcccgcgaca tcactaccgc ggccatcccg gcgaagagcg cggcctggac
     2641 aagcgcggcg attcgcagtg tcggcggaaa ctgtcccggg aacgcgccgc ccatcgcgta
     2701 cgcgccccac ggagcgccag cggccaaggc cacctgaaag gcgacgacgc ccgcggaaac
     2761 tgccgcatag atgatcgctg ccacacgtct gacgctcatg cggtcagggc gatggcgtag
     2821 agtgaatagg agatcaaggg atagt
//
LOCUS       DATAIR010000059         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_324534, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000059 DATAIR010000000
VERSION     DATAIR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324534"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VHM69_01535"
     CDS             complement(<1..693)
                     /locus_tag="VHM69_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2739100.1"
                     /translation="MRQETETGYFGPYGGRYVPETLIHALEELEEAYERYRNDPEFGE
                     ERDRLARDFVGRPTPLMFAERLTRKWGGANVWFKREDLAHTGAHKINNTLGQILLADR
                     MGKERIIAETGAGQHGVATATVAALYGKECVVYMGEEDTRRQRLNVVRMKLLGAEVVP
                     VLSGSRTLKDAVNEAIRDWVANVEDTHYIIGSVVGPAPYPRIVRDFQAVIGTEMEAQC
                     RERFGGDPEAIVA"
     gene            complement(677..1291)
                     /locus_tag="VHM69_01540"
     CDS             complement(677..1291)
                     /locus_tag="VHM69_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX2739101.1"
                     /translation="MMLVKVCGITNPGDARVAAEAGADAVGLLFAESPRRVSVEEARA
                     VCIALPENVLRVGVFVDEEPADILRISRELALDIAQLHGDETPETVTAVREGGVKVMK
                     ALRVRDAASLEALDAYEADLFLLDAFSEKARGGTGERFDWGVAKYLRGRDNIVVSGGL
                     GPENVREAVELFEPYGVDASSSLEDEPGRKNGKRVRRFVLAARD"
     gene            1503..2855
                     /locus_tag="VHM69_01545"
     CDS             1503..2855
                     /locus_tag="VHM69_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX2739102.1"
                     /translation="MRPEDIVTRAAEVLRENDMGGWTRASPTLYPHQWSWDSAFIAIG
                     LAHIDSGRAADEIRSLLEHQWKNGKVPHIVFNPEAPPESYFPGPEHWSSAAESPDAPP
                     DPDRTSGLCQPPVHAAAALRILQVAEARGEGVAEAREFLREVYPKLLAWHRYLLTQRD
                     PEGSGLVTIYHPWESGTDNSPRWSAALERVEVGEIPEYERLDLEHVPDPSERPTGLEY
                     DRFIWLVEVIKEARCDDAVLHETSPFLVKDVLASAILVRANEALLEIAGVANASDEDR
                     AQIRGWIERGRQGLEERWDPELGLCLDYDLRTGEPLRARTIAGFAALIAGAGEPDRLE
                     AHLRTLDSHAFTGNPDLRRPLPPSASPEELGFQPRNYWRGPVWPVMNWLLWWSLKKAG
                     ERKRAARLKSDALAQLSDGCFGEYYEPFTGEPLGSNEQSWTAAVALDWLAEEEMPENP
                     "
BASE COUNT          548 a         1048 c          976 g          545 t
ORIGIN      
        1 ggcgacgata gcttcggggt cgccgccgaa gcgctcgcgg cactgggctt ccatctccgt
       61 ccctataacg gcctggaagt cgcgcacgat ccttggataa ggcgcgggtc cgacgacgct
      121 cccgataatg tagtgcgtgt cctcgacgtt cgcgacccag tcccttatcg cctcgttcac
      181 ggcgtccttg agggtgcgcg agccgctgag tacgggcacg acctcggcgc cgaggagctt
      241 catccgcacg acgttcaggc gctggcggcg ggtgtcttcc tcgcccatat agacgacgca
      301 ttccttgccg tagagggctg cgacggtggc cgttgcgacg ccgtgctgac ctgcccccgt
      361 ttcggcgatg atgcgctcct tgcccatacg gtcggcgagc aggatctggc ctagagtgtt
      421 gttgatcttg tgcgcccccg tgtgcgccag gtcctcgcgc ttgaaccaga cgtttgcccc
      481 tccccacttg cgtgtcaggc gctctgcgaa catgaggggg gtcggacggc caacgaagtc
      541 cctcgccagg cggtcgcgct cctcgccgaa ctcggggtcg ttcctgtagc gctcgtacgc
      601 ctcttccaac tcttcgaggg cgtggatcaa agtctccggc acgtagcggc ccccgtaggg
      661 tccgaagtag cctgtctcag tctcttgccg caaggacgaa cctccgcacc cgcttgccgt
      721 ttttcctacc gggctcatct tcgagggaac tcgacgcgtc caccccgtag ggttcgaaga
      781 gctcgaccgc ctcccgcacg ttctccgggc cgagcccccc ggagacgacg atgttatcac
      841 gtcctcgcaa atacttcgca accccccagt cgaaccgctc tcccgtccct cccctcgcct
      901 tctcgctgaa cgcgtcgagc aggaagaggt cggcctcgta cgcgtccagc gcctccagag
      961 aagcggcgtc ccgtaccctc agagccttca tgactttgac tcctccctca cgcacagcgg
     1021 ttacggtctc aggagtctcg tcgccgtgga gctgcgcgat gtcgagcgcg agctcgcgtg
     1081 agatccttag aatgtccgcc ggctcctcgt ccacgaagac gcctaccctc agcacgttct
     1141 cggggagcgc gatgcagaca gctcgcgctt cttcgacgct cacgcgacgc gggctctcgg
     1201 cgaaaagcag cccgaccgcg tcagcccctg cctcagcagc cacgcgggcg tccccagggt
     1261 tggttatgcc gcacaccttc accagcatca tgaccgaagt gtactcctta tcttcggtgg
     1321 cgagaatcgg agccgtggat tgcggaaggt tcaattagaa ctgcctgggc ctgcggcgtg
     1381 atccgaggta gtcaatgact gcgtaggata tctgcaagcg ccgcgtaaga taagagtaca
     1441 attcgcggat ggagcgccga gagaatagct gccgacgggg accatagccg gaaggagtct
     1501 cattgcgacc tgaagatatc gttacacggg cggctgaggt gctgcgggag aacgacatgg
     1561 gcgggtggac ccgcgcctcc cccacgctct atccgcacca gtggagctgg gatagcgcgt
     1621 ttatcgccat agggctcgcg cacatcgact ccgggcgcgc ggcagatgag atccggtccc
     1681 ttctcgagca ccagtggaaa aacggcaagg tcccgcacat cgtcttcaac ccggaagccc
     1741 cgccggagag ctacttcccg ggtccagagc actggtccag cgccgcggaa tcccccgacg
     1801 ccccgccgga ccccgaccgt accagcgggc tctgccagcc gccggtgcac gccgcggcgg
     1861 ccctgcgcat cctccaggtc gcggaggcaa ggggcgaggg agtcgccgag gccagggagt
     1921 tcctgcgcga ggtatacccg aagctcctgg cgtggcaccg ctacctgctc acgcagcggg
     1981 accccgaagg ctccgggctc gtgaccatct accatccgtg ggagagcggc acggacaact
     2041 ctccgcgctg gagcgcggcg ctcgagcggg tcgaggtcgg ggagatcccg gagtacgagc
     2101 ggctggacct ggagcacgtt cctgacccgt cagagaggcc gacgggcctc gaatatgacc
     2161 ggtttatctg gctcgtcgag gttatcaagg aggcgcgttg cgacgacgcc gtcctgcacg
     2221 agacaagccc gttcctggtc aaggatgtac tggcgagcgc gatcctggtg cgtgccaacg
     2281 aagcgttgct ggagatagcc ggagttgcaa acgcttccga tgaggaccgg gcgcagatcc
     2341 ggggttggat cgaacgcggt cgccagggtc tcgaagaacg gtgggacccc gagctcggcc
     2401 tctgcctgga ttacgatctg cgcacgggcg aaccgctcag ggctcgcacc atcgcaggct
     2461 tcgcggcgct gatcgccggc gccggagagc ccgaccggct cgaagcccac cttcggaccc
     2521 tcgactcgca cgcgttcacg ggcaatcccg acctccgcag gccactgccg ccgagtgcca
     2581 gcccggaaga actcggcttc cagccccgca actactggcg tggtccggtg tggccggtta
     2641 tgaactggct cctgtggtgg tctttgaaga aggcgggcga gcgaaagcgg gcggccaggc
     2701 tcaagagcga cgccctcgcg cagctttcgg acgggtgctt cggcgagtac tatgagccgt
     2761 tcaccggcga gcctctggga tccaacgagc agtcctggac cgccgccgtt gccctcgact
     2821 ggctcgctga agaggagatg ccagagaatc cgtgaagata ggcccggtgc gccgacttct
     2881 cgcttcatac cgaatccggt tgaacaccgt cgcttttcac ccccgcagta gccacgcctt
     2941 ccaacccgtc attcccgcga aagcgggaat ccatgcccct tggagagccg tcatcgggag
     3001 cgactgagga cggtcgcgac agccaggcat ggagcgctat tatcccagat tcggtatgac
     3061 ccaaagacct ccgtcaaaaa cgccgcacga cccttgacac cagaccaatt cgagtat
//
LOCUS       DATAIR010000060         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_186148, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000060 DATAIR010000000
VERSION     DATAIR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186148"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VHM69_01550"
     CDS             complement(<1..100)
                     /locus_tag="VHM69_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2739103.1"
                     /translation="MATKRDYYEVLGVSREASEAEIKKAYRRAARSH"
     gene            complement(101..1129)
                     /gene="hrcA"
                     /locus_tag="VHM69_01555"
     CDS             complement(101..1129)
                     /gene="hrcA"
                     /locus_tag="VHM69_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX2739104.1"
                     /translation="MTHLVLSERQRELLLKTLEMYISTGAPVGSHALSDEVDASSSTI
                     RAELAYLESIGMLNHPHTSAGRVPTDRGYRYYVDALMEEKSRSETPFETLEGYGNVDE
                     LLRGVSEGMSEVTRLLAVVAGPSVLGESLARVDYLPLGGGRLLLVVSMESGASSSAAI
                     TLPFPVEDDELRGIFDALNSWLGGRPLGTDLELGLAARTALSDHDPRTVGAVLEAVGA
                     LGRATERGVFIQGASALLSRLDDLDPGSLSAVIEVFERRRWLLRLMSDALMRSVSSSG
                     VVVSIGTESGFVNLTGTSLVASAYSHRERPYGVVSLIGPKRMDYDVAITTVRSAAEAL
                     TNCLDARF"
     gene            complement(1175..2953)
                     /gene="lepA"
                     /locus_tag="VHM69_01560"
     CDS             complement(1175..2953)
                     /gene="lepA"
                     /locus_tag="VHM69_01560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX2739105.1"
                     /translation="MKNIERTRNFCIIAHIDHGKSTLADRLLDITEAVPERERMDQIL
                     DTMELERERGITIKAQAVRVLYKRDDGEWNLNLIDTPGHVDFAYEVSRAIAACEGALL
                     IVDASQGIQAQTLANLYLAMEHDLEIIPVLNKIDLPIADVAAVTEELVELLGVEEEEI
                     LKISAKTGEGVTDVLDAIVEHVPAPQTEHEETRALIFDSHYDPYRGVISLARLFDGEL
                     KKGDKVMAMGSEEQFELLEVGCYSPKPTALPALGAGEVGYVIAGLKDIEELRVGDTVT
                     RANDPASEPLPGYAKALPTVFSGLYPTEGDDFERLRDALSKLQLNDASLFFEPENSRM
                     GFGFRCGFLGLLHMEIIQERLEREFDLDLIASSPSVKYEVVVEGDIVEVTNPSELPEI
                     FDEIREPIVRATVICPKEYVGAVMGLCHEKRGVSVGMEYISTRRVQLTYDLPLAEIIT
                     DFFDALKSRTKGYASYDYEPTGYEPSDLVKVEVLVSGETVDALSIIVHRDKAYYRGRA
                     LVEKLKELIPRQQFEVPVQATMGKRVIARETVRAYRKDVTAKCYGGDITRKRKLLEQQ
                     KKGKRRMKQVGSVEIPQEAFLAALRI"
     gene            3037..3297
                     /gene="rpsT"
                     /locus_tag="VHM69_01565"
     CDS             3037..3297
                     /gene="rpsT"
                     /locus_tag="VHM69_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX2739106.1"
                     /translation="MPAPSKRDKQNVKRGAHNRGIRTRLRNLSKSFYRAVESGDAEKA
                     RQIRDQSQRAYNQAATKGVIHPNRAARKVSRLDKALAAKQGE"
     gene            3361..>3642
                     /locus_tag="VHM69_01570"
     CDS             3361..>3642
                     /locus_tag="VHM69_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739107.1"
                     /translation="MFALTPGDLSGRILDCAAGPASFNAELTSEGHEVISCDPLYSLS
                     ADEIRSRIDATRGTVVGNARAAQNEFVWRDFASPEHLGEVRMAAMQRFLA"
BASE COUNT          665 a         1170 c         1112 g          695 t
ORIGIN      
        1 ggtgagagcg ggcggcgcgg cggtaggctt tcttgatctc cgcctccgac gcctcccggg
       61 acacgcccag aacctcgtaa tagtcgcgct tggttgccac ctagaacctg gcgtccaggc
      121 agtttgtcag ggcttcggcg gcggagcgga ccgtcgttat agcgacgtcg tagtccatgc
      181 gcttggggcc gatcaggcta accaccccgt agggtcgctc gcggtggctg taagccgagg
      241 ctacgaggct cgtacccgta aggttcacga acccgctctc tgtgccgata gagacgacga
      301 cgccgctgct ggacaccgag cgcatgaggg catcgctcat aaggcgcagc agccacctgc
      361 ggcgctcgaa gacttcgatc accgccgaga ggctaccagg gtcgaggtcg tcgaggcggc
      421 tgaggagggc agatgcgccc tgtatgaaga ccccgcgctc cgtcgccctt cccagtgctc
      481 ctacggcctc gagcacagcc cctacggtcc tcgggtcgtg gtcagagagg gcggtgcgcg
      541 ccgcgagccc gagttccaga tccgtcccca gggggcggcc tccgagccag gagttcaggg
      601 cgtcgaagat gccacgcagt tcatcgtctt ccacggggaa ggggagtgtg atcgccgcgc
      661 tcgacgacgc gccgctctcc atcgacacaa ccagcaagag cctgcctccg ccgagcggaa
      721 ggtagtccac gcgggcgagc gactcgccga ggacgctggg cccggcgacc acggccagca
      781 ggcgtgtcac ctcgctcatc ccttcggaga cgccacgcag caactcgtcc acgttgccgt
      841 aaccttccag ggtctcgaag ggagtctcac ttctggattt ctcctccatc agggcgtcaa
      901 cgtagtaacg gtatcccctg tccgtaggga ctcgtcccgc cgaggtgtgt gggtggttga
      961 gcatgccgat cgactcgagg taggccagct cggcccgaat cgtggagctg ctcgcgtcca
     1021 cttcatcgga cagggcatgg ctacctacgg gggcgcccgt ggagatgtac atctccagcg
     1081 tcttcagtag cagctcgcgt tgcctctccg agagaaccaa gtgtgtcata tcgggcgtaa
     1141 ttttaacatg ggcgcgggat gtcccaaacg acgctcagat gcgtagcgcg gcgaggaagg
     1201 cttcctgcgg gatctccacg ctaccgactt gcttcatgcg gcgtttaccc ttcttctgct
     1261 gctcgagcag cttgcgcttc ctggttatgt cgccaccgta gcatttcgcc gtcacgtcct
     1321 tgcggtaggc gcgtacggtc tcgcgcgcga tcacacgctt ccccatcgtt gcctgcacgg
     1381 gcacctcgaa ctgctggcgg gggataagct ccttgagctt ctcgacgaga gcacgaccgc
     1441 gatagtacgc cttgtcgcgg tggacgatga tcgagagcgc gtccacagtc tcgccagaga
     1501 ccaggacttc gaccttcaca aggtcagacg gctcgtagcc cgtcggctcg taatcgtagg
     1561 aggcgtagcc cttcgtgcgg ctcttcaggg cgtcgaagaa gtccgtgatg atctccgcga
     1621 gcggcagatc gtaggtgagc tgtacgcggc gcgtcgagat gtactccatc ccgacggaga
     1681 cgccgcgttt ctcgtggcag agccccataa cggccccgac gtactcctta gggcagatca
     1741 cggtcgcgcg cacgatgggc tcacggatct cgtcgaagat ctccggcaac tcgctcgggt
     1801 tcgtcacctc gactatatcc ccttccacaa ctacctcgta cttcacgctc ggggatgaag
     1861 cgatgaggtc gaggtcgaac tcccgctcca atctctcctg gatgatctcc atatgcagaa
     1921 gtcccaggaa accgcacctg aacccgaatc ccatcctgct gttctccggc tcgaagaaca
     1981 gcgaggcgtc gttcagctgc agcttagata aggcgtctct gagacgctcg aagtcgtcgc
     2041 cttccgttgg atagaggccc gagaagaccg tcggaagggc cttcgcgtag ccgggcaagg
     2101 gctcggacgc cggatcgttc gcccgcgtca ccgtgtcgcc gacgcgcagt tcctcgatgt
     2161 ccttgaggcc ggcgatcaca tacccgacct cgcccgcgcc cagagcagga agggccgttg
     2221 gtttcggaga gtagcagccg acttccagga gctcgaactg ctcctcgctc cccatcgcca
     2281 ttaccttgtc gcccttcttc aactcgccgt cgaacaggcg cgccagcgag atgacgccgc
     2341 ggtaagggtc gtaatgggag tcgaagataa gggcacgcgt ctcctcgtgc tcggtttgcg
     2401 gcgccgggac gtgctccact atggcgtcga gcacgtctgt gacgccctcg ccggtcttgg
     2461 cggagatctt caggatctcc tcctcttcaa cacccaggag ttccaccagt tcctccgtaa
     2521 cggcggccac gtccgcgatg gggaggtcga tcttgttgag gacggggata atctccaggt
     2581 cgtgctccat cgccaggtag aggttcgcga gcgtctgggc ctgtatgccc tggctcgcgt
     2641 ctactataag tagcgcccct tcgcaggcgg ctatcgcccg cgagacctcg taagcaaaat
     2701 cgacgtggcc cggggtgtcg atcaggttca ggttccactc gccgtcatcc ctcttgtaca
     2761 ggacgcggac ggcctgggct ttgatcgtga tgccacgttc acgttcgagc tccattgtgt
     2821 ccaggatctg gtccatccgc tcgcgctcgg ggacggcctc ggttatgtcc aggagcctgt
     2881 cggcgagggt gctcttgccg tggtcaatat gagcaattat gcagaagttg cgcgtacgct
     2941 cgatgttttt cacctgtaga ttttaccatg attgcccctt tgctctacat gtgttacatt
     3001 ctctcggtta gcccagtatc aaatcggagg agcagcgtgc ccgcaccatc caagcgcgac
     3061 aagcagaacg tcaagcgcgg cgcccacaat agaggcatca ggacccgtct acgcaacctc
     3121 tcgaagagct tctatagagc cgtcgagtcg ggtgacgcag aaaaggcccg ccagatacgg
     3181 gaccagtccc agagggcgta caaccaggcc gcgaccaagg gtgtcattca cccgaacagg
     3241 gccgcccgca aggtcagccg cctcgacaaa gcactcgcag ccaaacaagg cgaataagca
     3301 acagttccca ggctggatga ggtagttccc tggggaagat ccaggggcga atacgtcagg
     3361 atgttcgccc tgacgcccgg cgacctctca ggcaggatcc tcgactgcgc cgcgggcccc
     3421 gcgagcttca acgccgaact cacgtcggag ggccacgagg tcatctcatg cgatccgctc
     3481 tacagcctct ccgccgacga gattcgatcc cgcatcgacg ctacccgtgg caccgtcgtg
     3541 ggcaacgcgc gggcagccca aaacgagttc gtctggcgcg actttgcatc accggagcat
     3601 ctgggcgagg tacggatggc ggccatgcag cgattccttg cg
//
LOCUS       DATAIR010000061         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_280486, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000061 DATAIR010000000
VERSION     DATAIR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280486"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..744)
                     /locus_tag="VHM69_01575"
     CDS             complement(229..744)
                     /locus_tag="VHM69_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739108.1"
                     /translation="MEKRPDRDPEPRRSSHHGGWDSDDFFGMDVEKMSPKAQAVTGVV
                     TGGLILLAAALLIAFTNFWWLIFVFGWMVFPAFGVFARGVAGLVETRSEPPAANARER
                     ELLEALQRRGELTPAQAAMETSLTVNEADKMLEDLAAKGHLDVKVRGGGLFYRLWETD
                     PEAGRREIGEA"
     gene            861..>1585
                     /locus_tag="VHM69_01580"
     CDS             861..>1585
                     /locus_tag="VHM69_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2739109.1"
                     /translation="MDLRERVDGSRDELLEELSEFLRMPSISARQDGSGDFRDCAQWV
                     ARKLEEAGAEARIMETDGHPVVYAEIGEGQKTLLSYGHYDVQPPEPLDLWESAPFEPI
                     VRDGNLYARGVADDKADVLARIQALRLYIEEQGPLPFKLKFLIEGEEEIGSPNLAPFV
                     RSNAELLSADACLWEGSLKDEAGRPMIFCGTKGMAYVELRARGASYDLHSMYGGIAPN
                     PAWRLVQALRTIKDENGEITLDGL"
BASE COUNT          313 a          525 c          478 g          269 t
ORIGIN      
        1 ccgcgtgagc ggtactcctc ccgcctcagg tggtagactt gctcctggta gaagtccctc
       61 tcctgaagcg agtcggccgt atcccgcaga cggtaggcct cctcaagcgt ctcaggtatc
      121 ctgcgatctt ccatgaacca gtgtttaccc tatgctctcg atctcacgcg ccgaaaaccc
      181 cttccgggtc ctgaatgata ccatgagcgc gttaaggcag aaacccaatt acgcctctcc
      241 gatctcccgt ctgccggcct cgggatcggt ctcccacaac cggtagaata gccctccccc
      301 acgcaccttg acgtcgaggt gacccttggc ggcgagatcc tctagcatct tgtccgcctc
      361 gtttacggtg agcgaggtct ccatcgccgc ctgcgccggc gtcaattcgc cgcgcctctg
      421 caaggcctca agcagctcac gctccctggc attcgccgcc ggaggctcag agcgggtttc
      481 gaccaggcca gcgacccccc tggcgaagac gccgaaagcg gggaagacca tccagccgaa
      541 gacgaagatg agccaccaga agttagtaaa cgctatcagg agtgccgcgg cgagaaggat
      601 caaaccaccc gtcaccacgc ccgttacggc ctgcgccttc gggctcatct tctccacgtc
      661 catgccgaag aaatcgtcgg aatcccatcc gccgtggtgg ctactacggc ggggctcagg
      721 gtcgcggtcc ggtctcttct ccacgggctt acctccagac tgaatatatc agcaaccccc
      781 cggatgaacc acgctcgaag acgcgaagcg cagacgcgaa agtgctaacc tcagcgcagt
      841 cgcgaaggag gctttggcgc atggacttga gagaacgcgt ggacgggtcg agagacgagt
      901 tactcgagga gctgagcgag ttcctgcgga tgccttcgat ctccgcccgc caggatggga
      961 gcggagattt tcgcgactgc gcacagtggg tcgcgcggaa gctggaagag gccggcgcgg
     1021 aggcccgtat catggagacg gacgggcatc ccgtggtcta cgcggagata ggcgaaggac
     1081 agaagacgct cctctcctac ggccactacg acgtgcagcc gccggagcct ttggacctct
     1141 gggagagcgc ccctttcgag ccgattgtca gggacgggaa cctatacgcc cgaggcgtcg
     1201 cagacgacaa agccgacgtg ctcgcccgca tccaggccct gcgtctatat atagaggagc
     1261 agggtccgct gccgttcaaa ctcaagttcc tcatcgaggg agaggaggag atcggcagcc
     1321 ccaacctcgc gcctttcgtc cgctccaacg cggagcttct ctccgcggac gcctgcctgt
     1381 gggaaggctc gctaaaggac gaggcggggc ggcccatgat cttctgcggc accaaaggga
     1441 tggcctacgt ggagctgcgc gcccgcggcg cctcctacga cctgcacagc atgtacggcg
     1501 gcatagcccc gaacccggcc tggcggctgg tccaggccct caggaccatc aaggacgaga
     1561 acggtgagat caccctggac ggcct
//
LOCUS       DATAIR010000062        18350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_372401, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000062 DATAIR010000000
VERSION     DATAIR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18350
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372401"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VHM69_01585"
     CDS             <1..519
                     /locus_tag="VHM69_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739110.1"
                     /translation="DEGIFFINGPFGVGKLLVKKVPDAMLYDPERIGAVLMRVLGPFK
                     RVDDFQGYALWRIRFVECARLLRKTSARALVVPMTVWRRDYFDRIIAGIHRVDPALAC
                     FRLTASRDVLMDRISSDTEDREARAWRTSHADVCLKALRDPAFGTEIRTDDRKPEEVA
                     DRILQIVGMPTG"
     gene            532..4305
                     /gene="metH"
                     /locus_tag="VHM69_01590"
     CDS             532..4305
                     /gene="metH"
                     /locus_tag="VHM69_01590"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX2739111.1"
                     /translation="MVLQRTKDEERQARLSEFRALLKQRIVLLDCAMGTMIQSYGLSE
                     EDYRGERFADHPGELKGNNDLLSITQPQIIRDIHEAAMEAGSDIIETNTFSSTSIAQA
                     DYEMEDLAYELNYEAARVAREAADAYATPERPRFVAGALGPTNRTASLSPDVNNPGFR
                     NITFDELAGAYGEAARGLVEGGADLLLVETIFDTLNAKAAIFGITAYLEEEGLDVPIM
                     ISGTITDASGRTLSGQVTEAFWNSVRHANPISVGLNCALGSAELRQYIEELSRISDVH
                     VSAYPNAGLPNEFGEYDETPEMMAHEIGEWAEAGWLNIVGGCCGTTPEHIRAIEESVS
                     GHPPREIPEIPKKLRLSGLEPCNVGPDSLFVNVGERTNVTGSARFKDLILNDDYETAL
                     DVARQQVENGAQAIDVNMDEGMLDGEEAMVRFLNLVAAEPDISRVPVMIDSSKWSIIE
                     AGLKRVQGKAVVNSISMKEGEEEFVRQARLLKRYGAAVIVMAFDEKGQADTRQRKVEI
                     CKRAYKILTEEVGFPAEDIIFDPNVFAIATGIEEHDNYGVDFIEAVREISQTLPYANT
                     SGGISNVSFSFRGNNPVREAIHAVFLYHAINAGLTMGIVNAGQLAVYDEIDSELREAV
                     EDIVLNRRPDSTERLLGLAEKYRELGDVSADEKDQEWRSWPVEKRLEHALVKGIAEFV
                     EVDTEEARQQATRPLDVIEGPLMDGMNVVGDLFGSGRMFLPQVVKSARVMKKAVAYLI
                     PFIEEEKGEARKPNGTIVMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVPSTKIL
                     ETAREKGADIIGLSGLITPSLDEMVHNAKEMQREGFDIPLLIGGATTSQTHTAVKIAP
                     GYEQPVIHVKDASRAVGVVQNLVSEGRKAGYAEQIAADYEQVRKKHAGRRSKTRLTTL
                     ERARANRMKIDWEDYTPAKPNVLGVRTFDDYALEEIRPYIDWTPFFHSWQLKGSHPRI
                     FDDPQKGEEARKLFADAQELLDRIIAEKWLTANAVFGLFPASALPNDSIEVYTDESRE
                     EVLTNLQFLRQQKQKPPGRPNHSLADFVAPKSTGLEDHIGLFAVTAGLGADEAAHRFE
                     EDNDDYNAIMVKALADRLAEAFAELLHSRVRREFWGYASDEKLGSEALIREEYRGIRP
                     APGYPACPEHTEKRTLWSLLDVEANAGITLTESCAMSPGAAVSGFYFSHPDSRYFGVS
                     DIGRDQASDYAKRKGWTLEEAEKWLAPNLVYDPEEPGGNGLMSVPQARSKSAG"
     gene            complement(4366..5043)
                     /locus_tag="VHM69_01595"
     CDS             complement(4366..5043)
                     /locus_tag="VHM69_01595"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEX2739112.1"
                     /translation="MLELFGILAFFVLAILVGADIYVGMSNFGYGWLLPVLAVVAYLA
                     ADLLSGVVHFLADNFGSAETPVIGPNFVGAFREHHVDPKGITTHDFIDTNGNNSLASL
                     PFMGLVWLAVPVDTTAGGYLFGAFCLMLCVATFLTNQFHKWAHMDEPPSPVVWLQRRG
                     LILSSEHHDIHHASPYDTYYCITVGVWNPLLDRTRFFERAERLLRRIVPGTDPHLRVE
                     REGSLND"
     gene            complement(5162..5797)
                     /locus_tag="VHM69_01600"
     CDS             complement(5162..5797)
                     /locus_tag="VHM69_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX2739113.1"
                     /translation="MGKKGTGLGTTLVLALVIVCVSVGLGIGISRFDFMEGVPVLGPL
                     LEEKPARTTTGPVVVEGIQELDQLATVRWTESVPVTRESGGDALERLFSGEKVLVIAT
                     GEVEAGVNLADIDKDDVSVDGDTVTIDLPEPEILSSSLDEKTTRVYDRDFSPLNLRPD
                     DDLVEEARARAVEKVEEAALENEILVTAEQNAEDSIRAFVITLGFERVRFE"
     gene            complement(5863..6054)
                     /locus_tag="VHM69_01605"
     CDS             complement(5863..6054)
                     /locus_tag="VHM69_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEX2739114.1"
                     /translation="MARYRPYGPAFKPGEKAPISGTYICDRGTMLPPVRVQLSKGEEF
                     PEAGGLGEHTRWRETNIQA"
     gene            complement(6075..7643)
                     /gene="serA"
                     /locus_tag="VHM69_01610"
     CDS             complement(6075..7643)
                     /gene="serA"
                     /locus_tag="VHM69_01610"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX2739115.1"
                     /translation="MKVLVPEKLADPGIELLKRDFEVDVLLDLTPEDLLERIGEYDGL
                     IIRSATRVTAEVIERAENLKAIGRAGIGVDNIDIEAATKRGIIVANAPESNTVAAAEH
                     TLGLMLAAARRIPAADSTLRGGEWKRSAFKGVEVSGKTLGLVGLGHVGAIVARGALGM
                     GMRVLAYDPYVSEDRMRSMNVERAETTDEVFEHSDFVSLHVPRTPQTLGLVNEDSLAK
                     MKPTAYIINVARGGIVDETALYNALKGGVIAGAALDVFAEEPTTDSPIFTLPNVVVTP
                     HLGASTAEAQDRAGITAAEQVATALRGTVPMNAINAPVPMGEGAEFVAQFSGLCESLG
                     SLLYQLTDHPGDTLRIEYRGEVAGFDTRLLDVSAQKGLLAPMIFEPLNFVNTPALAKE
                     RGLKVETSRSSESADYTSLVELRMGENAVSGTLFGPRMQPRLVEAMGFDFDVVPEKYM
                     LFIRNEDRPGMIGKLGSILGEHGINIGNMAVGRGEPGSRAAMAVTVDEPVPEDVLNAL
                     VETPGFNEARSVTL"
     gene            complement(7640..8797)
                     /locus_tag="VHM69_01615"
     CDS             complement(7640..8797)
                     /locus_tag="VHM69_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX2739116.1"
                     /translation="MMHKYRLMSPGPTPIPPEVSAAGALPIIHHRTPEFGEVFTRVNE
                     NLKRVFLTENDIFTYAASGTGAFEGAIQNLFSPGDKVLVVNNGNFGGRWVKMSRAFGL
                     EVTELKYDWGVKADNDEVAEALANDPEIKAAICVLSETSTGTVNDIEGFAKAASDVVT
                     IVDAVSGLGACTLRTDEWGVDVVVAGSQKALMTPPGLGFVSVSERAMRMHEESRMPRF
                     YFDWTAARKAYEKDPAQTAWTPAVSLIIQLDVALQQLIDEGVENVFERHVLLGRASRE
                     GIKGMGLKLFGPDEDLNSAVTAAWVPDGIDGKQLVRMVFREHGIQVAGGQGEMAGRIF
                     RIGHCGYFDAFDIIATIAATELALESLDYPVELGKGVGASQRVFSKSGVTA"
     gene            complement(8936..13384)
                     /locus_tag="VHM69_01620"
     CDS             complement(8936..13384)
                     /locus_tag="VHM69_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2739117.1"
                     /translation="MAVGWDYRLVVLSYVIAAFASYTALDLAGRVTATGGRGRIAWLA
                     GGAFAMSMGIWSMHFTGMLALKLDMPITYDVLGTLLSMAVAIAAATLAFFVVSRGVVG
                     VRELALAGPLMGVGIASMHYTGMAAMRMQATVRYDLLLVATSFVIAIGASVAALWLFL
                     RLNGGDLTRGTRTLLKAVSALVMGTAVAGMHYTGMAAATFMHTSERAAPSNDLNTTVM
                     GFGIGTFTLLILGLALISAFIDRRFSAQAAQLEDSEARYSRIVANAPGMVYQMVRRQD
                     GSIALPFVSRGSREIYGLEPHELQQDPSLYIDAIHPEDRPSFDLALAESAATLSPCEW
                     EGRVRLRSGEQKWLRKASRPQRRANGDIVWDGLLMDVTERKRAESELEETEGRFRNIF
                     ENAPIGMSLTSLDNRYLQVNQALCDMLGYSEEELLSKRSREVTHPDDREITTTRTRVL
                     LEGKVKRDLVQKRYIRADGDIVWAISSVSLVRDSRGDPAYFVGQYQDISERKRAEEAL
                     RESEEFFRALYERVHHPIFLLDEHLNFIDVNPYACEFYGYAREEFRRMNVSDITVSEE
                     HAGQRNHARRMRHGDEALIQERRHRKKGGELAVVTADVMAVTRAGQRLYVSKITDITA
                     LTRTEEEIRHLNEELEERIERRTEQLETIVSELRENERRLVESEERYSLVVKVSNDGV
                     FDWKLGTGELYWNERLYEIAGLSPSEVVPSLELFEDHLHPEDRHRVIEAITAHFERDE
                     AYALECRVRRPSGEYRTCIVRGEAQRDEQGNPVRMSGVVRDITERKSTEEEIRRLNEE
                     LEERIERRTEQLQSAVTELEQAGDELRSAKEAAEAANRAKSEFLANMSHEIRTPMNGV
                     IGMTGLLLDTDLSEEQLEYAGTIRTSGEHLLTIINDILDFSKIEAGMMEFEVIDFDLR
                     NTVEEALDLFVEGAHAKGLELATLVTYDVPEVLRGDPGRLTQVLTNLIGNAIKFTEEG
                     EVVVRAELSEARDDSAIVRFSVTDSGIGMTSEQQVRLFTSFTQADASTTRKYGGTGLG
                     LSISSQLVELMGGEMGVESAPGEGSTFWFELPFDMQPEAAGRPRQPHPDLGDLRVFVV
                     DDNETNRKILHQQILSWGMENGMAEDAQSALELLRIADEDGDPYDVAILDMQMPGMDG
                     MELARRINDDPDLQSTRLILLTSLGIRGDARKAREAGIDAYLTKPVRQSHLLDAIATV
                     MGSTEAEEGEAHLVTQHSIGDERARERARILLAEDNAINQKVAVKLLESLGYRRVDVA
                     ANGIEAVDALSRVPYSAVLMDCHMPEMDGYEATAEIRRREKEGHTPIIALTAGAMKGD
                     RERAIDAGMDDYIPKPVKREDLETMLERWVSQEDVATLAGAQDGRSSAREEDRLDHAV
                     IANLRELGDSDLLSELADVFVEEVPDMLDTLREAIEKGDARAVQRIGHTLKGSSGNMG
                     AKRMSRLCLDLEHAGESNDIPAAARLLEALREEFDHFRTELSTLVG"
     gene            13629..14417
                     /locus_tag="VHM69_01625"
     CDS             13629..14417
                     /locus_tag="VHM69_01625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2739118.1"
                     /translation="MDHDEVGRYWDENADVWTELVRAGYDHYRDGLNSPAFLDMLPDV
                     NSLSGLDVGCGEGHNTRLLAQRGARITAIDISRTFIRYAREAEEGHPLGIRYQTASAV
                     DLPFDEASFDFATAFMSLMDIPETGRVLAEVFRVLKPGGFFQFSITHPCFDTPHRKNL
                     RDESGRTYAIEVGDYYRRQRGEVEEWIFSAAPPEVREGLRPFRMPRFTRTLSDWLNLL
                     VETGFMLERFGEPYPSDEAVRERPGLQDAQVVAYFLHVRARKPA"
     gene            complement(14414..15469)
                     /locus_tag="VHM69_01630"
     CDS             complement(14414..15469)
                     /locus_tag="VHM69_01630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2739119.1"
                     /translation="MEVSCSGMDLDALLLDAVHPEDLAGMLMAALRLNLPTVCVPASG
                     KPFSAALVALGLAPISGEPAGTAVEASDGPRPRELVENFSLANALRAGVAARGGPEIL
                     VHLAAIAREGGVAGFAQMVRVLASETPEAAPDWLREHGVAGLLASLGDSLHDVRTVAG
                     NLKEKLPTLPPPPGEHSRLVFVMARASGAEGVCRVHGGVTEVAGECRVFGSEEEAVLV
                     VRRGDVEDGTMLVVGGFGPRGGPGLLRLDALGRALREARLEVPVLTDGLAPEDARGAW
                     VSLFTPEAAAGGVLSRLRDGDPIRIDFADCRIRTGVGAREFEAREPREFPERAGTGYA
                     ARYARTALPALEGAGFG"
     gene            complement(15635..17092)
                     /gene="gatB"
                     /locus_tag="VHM69_01635"
     CDS             complement(15635..17092)
                     /gene="gatB"
                     /locus_tag="VHM69_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX2739120.1"
                     /translation="MEVQEKRTYQAVIGLEFHVHLATRTKMFCGCSVTYGEAPNTHTC
                     TVCLGHPGALPVTNEKAVELGVMAGLALNCDVAGRAVFARKNYFYPDLPKGYQISQFD
                     EPICIGGYIDVPTSEGRIRVGITRLHLEEDAAKNVHVGTSGRMHGSVGSLIDFNRGGT
                     PLMEIVTEPDIPSPEAARATANQLKEILRAVGVSEADMEKGQLRCDANVSIRNPDGSF
                     GTKTELKNMNSFRFVERGLARELERQREILENGGRVEQTTLHYDPETDEVHELRSKEY
                     AHDYRYFPEPDLLPLEMSPARVREIGERLPELPDQMRARFVQQYALSEYDAGVLTADR
                     DLALFYEAVAAEVDPKQSANWISGDLRALLNETGTDISDSKVTSGHMIELIELVAAGK
                     VSRSAAKDVLASVFETGDSPSAVVEREGLASVGSDELSGVVEEAISANPDEAERVRNG
                     DKKVVGFLVGQVMKATRGNADGGRVRQLLMEKLGS"
     gene            17178..18074
                     /locus_tag="VHM69_01640"
     CDS             17178..18074
                     /locus_tag="VHM69_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2739121.1"
                     /translation="MALDGGYVSLHTGIENDFAGVNRPHLVVLQGPGLGDFFEIDDDP
                     LVLGSDPFRADIVVRDVEVEPRHAEVYREPGGYAVRGVGARKSFALNGRVIEELGGGH
                     LLSDGDRIHLGDSVLEFSHQDEIKASFYEQLHHLVNRDHLTGLYSKGRFDREFEHRLE
                     AMADEGRPLGVLMADIDSLKKINDAHGHLLGEFVVGEIGRIIGTLHEDEGRLATRFGG
                     DEYQTILPDLSKEQAIEVAEEIRRRVASHTFERGDVAARTTISLGVATYPEDGKTRQE
                     LTHAADAALYRAKRAGGNTVSE"
BASE COUNT         3392 a         5878 c         5672 g         3408 t
ORIGIN      
        1 gatgagggga tcttctttat caacggcccg ttcggcgtcg ggaagctgct cgtcaagaag
       61 gtcccggacg cgatgctcta cgacccggag agaattggcg ccgtcctcat gagagttctc
      121 ggccccttca agagggtcga cgatttccag ggctacgcgc tctggcgcat acggttcgtc
      181 gaatgcgcac gccttctgag gaagacgtca gcgcgggcgc tggttgttcc catgacggtg
      241 tggcgccgtg attacttcga ccgtatcatc gccggaattc acagggtaga tccggctctg
      301 gcgtgtttcc ggctgaccgc ctccagggat gtactgatgg atcgcatctc gtccgatacc
      361 gaggataggg aagcccgtgc gtggcgcacg tcccacgccg atgtctgcct gaaggctctg
      421 cgcgacccgg ctttcggaac ggagatacga acggacgacc gtaagcccga agaggttgcg
      481 gaccggatcc tgcaaatcgt gggtatgccg accggctaga atagggggtt catggttctc
      541 cagcgcacga aggacgaaga gagacaggcc cgattgagcg agttccgggc gctcctgaag
      601 cagcgcatcg ttctgctcga ctgtgcgatg gggaccatga tccaatccta cggactctcc
      661 gaggaggact acaggggaga acgcttcgcc gatcatcccg gagagctcaa aggcaacaac
      721 gacctgctct cgatcaccca gccacagata atccgcgaca tccacgaggc ggcgatggaa
      781 gcgggctcgg acatcatcga gacgaacact tttagctcga cctccatcgc ccaggctgac
      841 tacgagatgg aggaccttgc ctacgagctg aactacgagg cagcccgcgt agcccgcgag
      901 gctgctgacg cctacgcgac cccagagagg ccgcgcttcg tggctggcgc tctcggcccg
      961 acgaaccgta ccgcgtcgct ctcacccgac gtcaacaacc cgggcttcag aaacatcacc
     1021 ttcgacgagc ttgcaggggc gtacggtgaa gccgcgcgag gactcgtcga gggcggcgcg
     1081 gacctgctgc tcgtggagac catcttcgac accttgaacg ccaaggctgc catattcggg
     1141 atcacagcct acctcgaaga ggaaggcctc gacgtgccca tcatgatctc cggcacgatc
     1201 acggacgcga gcggacgcac gctctcgggg caggtgacgg aggctttctg gaactccgtg
     1261 cgccacgcga accccatcag cgtcggcctg aactgcgcgc tcggctccgc ggagctcagg
     1321 caatacatcg aggagctatc caggatctcg gacgtccacg tgagcgccta cccaaacgcc
     1381 gggctgccca acgagttcgg agagtacgac gagacccccg agatgatggc acacgagatc
     1441 ggggagtggg ccgaagcagg ctggctcaac atcgtcggcg gctgctgcgg cacgacgccg
     1501 gaacacatca gggcgatcga agaatccgtc tccggccacc cgccgcgcga gataccggag
     1561 atccccaaaa agctcaggct atcggggctc gagccttgca acgtcgggcc tgattccctg
     1621 ttcgtgaacg ttggcgagcg gaccaacgtg accggttcgg cgcggttcaa ggacctcata
     1681 ttgaacgacg actacgagac ggccctcgac gtagcgcgcc aacaggtcga gaacggtgcg
     1741 caggccatcg acgtcaacat ggacgagggg atgctcgacg gcgaggaggc gatggtccgc
     1801 ttcctcaacc tcgtggccgc cgagcctgat atctcgcggg tgccggtaat gatcgactct
     1861 tccaaatggt cgatcatcga ggccggcctc aaaagggtgc agggcaaggc cgtcgtcaac
     1921 tccatcagca tgaaggaagg cgaggaggag ttcgtcaggc aggcgcggct cctgaagcgg
     1981 tacggggcag cggtaatcgt aatggccttc gacgagaagg gccaggccga caccaggcag
     2041 cggaaggtcg agatctgcaa acgcgcctac aagatcctta cggaagaagt cggcttccct
     2101 gccgaggaca tcatcttcga ccccaacgtc ttcgccatcg ccacgggcat cgaggaacac
     2161 gacaactacg gcgttgactt catcgaggct gtgcgtgaga tttctcagac cctcccctat
     2221 gcgaatacat ctggtggtat ctcgaacgtg tcgttctcgt tcaggggcaa caacccagtg
     2281 cgggaggcga tccacgccgt cttcctctac cacgccataa acgcaggact gacgatgggc
     2341 atagtgaacg ccggccagct cgcggtctac gacgagatcg actctgagtt gcgcgaggcc
     2401 gtcgaggaca ttgtcctgaa ccgccgcccc gactctacgg aacgcctgct cgggctggcc
     2461 gagaagtacc gtgaacttgg cgatgtctcc gcggacgaaa aagatcagga atggaggagc
     2521 tggcccgtcg agaagcggct ggagcacgct ctggtcaagg gcatcgcgga gttcgtcgag
     2581 gtggacacgg aggaagcacg ccagcaagcc acacgtccgc tcgacgtgat cgaaggtcct
     2641 ttgatggacg ggatgaacgt ggtcggcgac ctgttcgggt ccggcaggat gttcctcccg
     2701 caagtcgtca agagcgcccg cgtgatgaag aaggccgtcg cctacctcat cccgttcatc
     2761 gaggaggaga agggcgaggc ccgcaagccg aacggcacga tagtgatggc gaccgtcaag
     2821 ggcgacgtgc acgacatcgg gaagaatatc gtcggggtcg tgctccagtg caacaacttc
     2881 gaggtaatag atctcggcgt gatggtgcca tctacgaaga tcctggagac ggcccgcgag
     2941 aagggcgccg acattatcgg gctctcgggc ctcataacgc cttcgcttga cgagatggtc
     3001 cacaacgcga aagagatgca acgcgagggc ttcgacatac ccttgctcat cggcggggcg
     3061 acgacctccc agacgcatac ggccgtcaag atagcgccgg gctacgagca gcccgtgatc
     3121 cacgtaaaag acgcctcgcg cgcagtcggc gtggtccaga acctggtgag cgagggacga
     3181 aaagctggct acgccgagca gatcgccgcc gactacgagc aggtccgcaa gaagcacgcg
     3241 ggacgccgct cgaagacccg cctcacgacg cttgagaggg cgcgcgccaa ccgtatgaag
     3301 atcgactggg aagactacac accagcgaaa ccgaacgtcc tcggcgtgcg gacgttcgac
     3361 gactacgcgc tggaggagat acgtccttac atagactgga ctcccttctt ccactcctgg
     3421 caactgaagg gcagccaccc caggatcttc gacgaccccc agaagggcga agaagcccgc
     3481 aagctcttcg ccgatgcaca ggagctgctg gaccgcatca tcgccgagaa gtggctgacg
     3541 gcaaacgccg tcttcggcct cttccctgcg agcgcgcttc cgaacgattc catagaagtc
     3601 tacacagacg agtcgcgcga agaagttctg acgaacctcc agttcctgcg ccagcagaag
     3661 cagaagccgc caggccgtcc caaccattca ctcgccgact tcgtcgcccc caagagcacg
     3721 ggtctcgaag accacatcgg gctcttcgcc gtcacagccg ggctcggggc ggatgaggcg
     3781 gcgcaccgct tcgaggagga caacgacgac tacaacgcca tcatggtcaa ggcgctggcc
     3841 gacagacttg ccgaagcctt cgccgagtta ctgcacagtc gggtccgcag ggagttctgg
     3901 ggttacgcgt cggacgagaa gctgggcagc gaggccctca tccgcgagga gtacaggggc
     3961 attcgtccgg cacccggcta tccagcctgc ccggagcaca ccgagaagcg tacactctgg
     4021 agtctgctcg acgtggaggc gaacgccggc atcacattga ccgagagttg cgcgatgtcg
     4081 cctggtgcgg cggtcagtgg cttctacttc tcgcacccgg attccaggta tttcggcgtt
     4141 tcggacattg ggcgggatca ggcatccgac tatgccaaac gcaagggctg gacgctggaa
     4201 gaggccgaga agtggctcgc cccaaacctg gtctacgacc cggaggaacc gggaggcaat
     4261 ggtctcatgt cggttccgca ggcccgcagc aagtccgccg gctaagggtt gccccggcat
     4321 tctcgccacc cgcgagagtc gagtccttac tcgaaacgat actctctagt cgttaaggct
     4381 tccctcacgc tcgacgcgga ggtgggggtc cgtgcctggt acgatccgac ggagaagtcg
     4441 ttcggcccgc tcgaaaaagc gggtccggtc gagtagcgga ttccagactc cgacggtgat
     4501 gcagtagtag gtatcgtagg ggctcgcgtg gtggatatcg tggtgttcgc tggagaggat
     4561 cagaccccgc ctttggagcc agacgacggg tgagggcggt tcgtccatgt gcgcccattt
     4621 gtggaactgg tttgtcagga acgtcgcgac gcacagcatg aggcagaacg ccccgaacag
     4681 atacccgcct gccgttgtgt cgacgggaac ggcgagccag accagcccca tgaaagggag
     4741 actagccagg ctgttgttgc cgttggtgtc gataaagtcg tgcgtcgtga tgcccttcgg
     4801 gtccacgtgg tgctcgcgaa aggcaccgac gaagtttggc ccgatgaccg gcgtctcggc
     4861 ggacccgaag ttatccgcca ggaagtgcac gaccccggag agcaggtccg cggccaggta
     4921 cgcaacgacc gcgaggaccg gcaggagcca cccgtagccg aagttcgaca tccccacgta
     4981 gatgtccgcc ccgaccagaa tcgccagcac gaagaaagcc aggattccga agagttccag
     5041 catgtaatga ctcctgccgg tcttgggacg aatccggcct cgcgctgcct gctgttggtt
     5101 gatcataggc acctcctagg aggaggatac ggaccatgag gtccgctaga tcaccaagtg
     5161 gctactcgaa ccgtactctc tcgaatccca gcgtgatcac gaacgcccgt atgctgtctt
     5221 ctgcgttctg ttcagcggta accaggatct cgttctccag tgcggcctct tcgactttct
     5281 ccaccgcccg cgctcgggct tcttcgacga gatcgtcgtc ggggcgaagg ttcagggggc
     5341 tgaaatcgcg gtcgtagaca cgggtcgtct tctcgtcgag gctggaggag aggatctcgg
     5401 gctccggcaa atcgatggtt acggtgtcgc cgtccacgct cacgtcatcc ttatcgatgt
     5461 cggcgaggtt cactccggcc tcgacctccc cggtggctat aacgaggacc ttctcgcctg
     5521 agaagagccg ctccagtgcg tcgccgccgc tctccctggt aaccgggacg gattcagtcc
     5581 agcgcacggt ggcgagctga tccagctcct gtattccttc cacaacgacg gggccagtcg
     5641 ttgtgcgcgc gggcttctcc tccaggagcg gaccgagaac gggcaccccc tccatgaaat
     5701 cgaagcggct gatgccgatg ccaaggccca cgctaacgca aacgatgacg agcgccagaa
     5761 cgagcgtcgt ccccaacccg gttcctttct tacccacaac ccgtcctctc aacctcgtgt
     5821 agatgcgcca attctacacc gtgcgaggac actgacagct attcaggcct ggatgttcgt
     5881 ctcgcgccag cgggtatgct ctccgagacc tccggcctcg gggaactcct cacccttgga
     5941 gagctggacc ctgacgggcg gcagcatcgt ccccctgtca cagatgtagg tacccgagat
     6001 cggtgccttc tcgccaggtt tgaaggccgg tccgtagggt ctgtagcgcg ccatgatttc
     6061 ttctaggctg gtcatcacag cgtgacggag cgggcttcgt tgaaaccagg cgtctccacg
     6121 agagcgttga ggacatcttc ggggacgggc tcgtccacgg tgacggccat cgccgcgcgg
     6181 gagcctggct cgccgcgtcc gacggccatg ttgccgatgt tgataccgtg ctcaccgagg
     6241 atgctgccga gctttccgat catgcctggc ctgtcctcgt tgcgtatgaa gagcatgtat
     6301 ttctccggca caacgtcgaa gtcgaagccc atcgcctcga cgagccgcgg ctgcatcctt
     6361 ggtccgaaga gggtcccgct taccgcgttc tcgcccatgc gaagctccac gaggctcgtg
     6421 tagtccgcgc tctccgagga gcgcgaggtc tctaccttca gccccctctc cttcgcgagc
     6481 gcgggcgtgt tgacgaagtt cagtggctcg aagatcatcg gcgcgaggag ccccttctgg
     6541 gcagaaacgt cgagtaggcg cgtgtcgaag ccagcgacct cgccgcggta ctcgatcctc
     6601 aaggtgtcgc cggggtggtc ggtgagctgg tagagcaggc tccccagaga ctcgcatagc
     6661 cccgagaact gggccacgaa ctcggcgccc tcccccatcg gcaccggcgc gtttattgcg
     6721 ttcatcggga ccgtgcccct gagggccgtc gcgacctgct cagcagccgt gataccggcc
     6781 ctgtcctgcg cctctgccgt gctcgccccg aggtggggcg ttacaacgac gttggggagc
     6841 gtgaagatcg gggagtccgt cgtcggctcc tccgcgaaaa catcgagcgc cgcaccggcg
     6901 atgacaccgc ccttgagagc gttgtacagg gccgtctcgt ccacgatacc accgcgcgca
     6961 acgtttatga tgtaagctgt cggcttcatc ttcgcgagcg agtcctcgtt caccaggcca
     7021 agcgtctgcg gcgtgcgcgg gacgtgcagc gagacgaaat ccgagtgctc gaagacctcg
     7081 tcagtagtct cggcccgctc gacgttcatc gagcgcatcc tgtcctccga gacgtagggg
     7141 tcgtaggcga ggacacgcat ccccataccc agagcgccgc gagccacgat cgcacccacg
     7201 tggccgagcc cgaccagacc tagcgtcttc ccagatacct cgacgccctt gaaggcgctc
     7261 cgcttccact ccccgccacg caatgtagag tcagcagcag ggatgcgccg cgccgcggcg
     7321 agcatgagcc ccaaggtgtg ctcggccgcg gccacggtgt tgctctctgg cgcgttggcg
     7381 acgatgatgc cgcgcttggt cgccgcctct atatctatgt tatcgacgcc gatgcctgcg
     7441 cgcccgatag ccttgaggtt ctcggcccgc tctatcacct ccgccgtgac tctggtggcg
     7501 ctgcggatga tcagaccgtc gtactcccct atcctctcca gcaggtcttc cggagtcagg
     7561 tcgaggagga cgtctacctc gaaatctctc ttcagaagct cgatgcccgg gtcggcgagc
     7621 ttctcgggga cgaggacttt caagcggtca ccccgctctt cgagaagacc ctctgggacg
     7681 cgccgacgcc cttgccaagc tcgacggggt aatcgaggga ttcaagggcc agctccgtgg
     7741 ccgctatggt cgcgatgata tcgaaggcat cgaagtagcc gcagtggccg atgcggaaga
     7801 tcctgccagc catctcgccc tgcccgccgg cgacctgtat tccgtgctcc ctgaagacca
     7861 tgcgcacgag ctgcttgccg tctatgccat ccggcaccca cgccgccgtg acggccgagt
     7921 tcaaatcctc gtccggcccg aagagcttga gccccatgcc cttgatgccc tccctcgatg
     7981 ccctcccgag caaaacgtgc cgctcgaaga cgttctcgac accctcgtcg atgagctgct
     8041 gcagcgcgac gtcgagctgg atgatgaggc tcacagcagg ggtccacgcg gtctgcgcgg
     8101 ggtccttctc gtaggccttc ctggccgccg tccagtcgaa gtagaagcga ggcatcctgg
     8161 actcctcgtg catccgcatc gcccgctcgg aaacgcttac gaacccgagc cctggcggcg
     8221 tcatcagcgc cttctgcgag cctgccacga ccacgtcgac gccccactcg tccgtcctca
     8281 aggtgcatgc cccgagccca gagaccgcat ccactatcgt cacgacatcg gatgcggcct
     8341 tcgcgaagcc ctcgatgtcg ttcacggtgc ccgtcgaggt ctcagagagg acgcagatgg
     8401 cggctttgat ctccgggtcg ttcgccaggg cttcggcgac ctcgtcgttg tccgccttca
     8461 cgccccagtc gtacttcagc tcggtgacct caaggccgaa ggcccggctc atcttgaccc
     8521 acctgccgcc gaagttgcca ttgttcacga cgaggacttt gtctccagga gagaagaggt
     8581 tctggatcgc cccctcgaaa gcccccgtac cgctcgccgc gtacgtgaag atgtcgttct
     8641 cggtcaggaa gacccgcttc aagttctcgt tcacccgcgt gaacacctcg ccgaactccg
     8701 gcgtgcggtg gtggatgatc gggagcgccc cggccgcgga tacctcaggc ggtatgggag
     8761 tcggacctgg cgacataaga cggtatttgt gcatcatggg tcgggaggtt tcctctcttg
     8821 cagcggacaa ctcacgccta ttctataaat tattgctcca gatgactaca tgatggcgca
     8881 gctaatttac aaagtgttgc ttttgccttg caaggtgcat cctgatccgg agggctcatc
     8941 ccacgagtgt cgagagctcc gtgcggaagt ggtcgaactc ttcccttagc gcctcgagga
     9001 ggcgggctgc ggctgggatg tcgtttgatt cgccggcgtg ttcgaggtcg aggcacagcc
     9061 tggacatccg ctttgctccc atattgccag agctgccttt gagggtgtgc ccgatgcgct
     9121 gcacggcccg cgcgtcgccc ttctctatgg cttccctgag ggtgtcgagc atgtcaggca
     9181 cctcttcgac gaacacgtca gcaagttcgg agagaaggtc cgaatctccg agctcgcgca
     9241 ggttcgctat gacggcgtgg tctagcctgt cttcttcccg tgcggatgat cggccatcct
     9301 gcgcgccggc taaagttgcg acgtcctcct gcgaaaccca tcgttcaagc atggtctcga
     9361 gatcctcgcg cttgacgggc ttgggtatat agtcgtccat gccggcgtcg atggccctct
     9421 ccctgtctcc cttcatagcg ccggcggtca gtgcgattat cggcgtgtgg ccttccttct
     9481 cgcgccggcg gatctccgcc gtggcctcgt agccgtccat ctcgggcatg tggcagtcca
     9541 tgaggacggc cgagtagggg acccgcgaga gggcgtccac ggcctcgatg ccgtttgcgg
     9601 caacgtcaac gcgacggtaa cccaggcttt cgagcaactt cacggccact ttctggttta
     9661 tggcgttgtc ctcggcgagt agtatgcgtg cccgctcccg cgctctctca tcgccgatag
     9721 agtgctgggt gaccagatgt gcctctccct cctcggcctc cgttgagccc atcaccgtgg
     9781 ctatagcgtc gaggagatgg gactgcctca ccggcttggt aaggtaagca tcgatgccgg
     9841 cctctcgcgc cttcctggcg tccccgcgga tcccaaggga ggtgagcagg atcaggcgtg
     9901 tagactggag gtcggggtca tcgtttatcc tgcgcgccag ctccatgccg tccatgccgg
     9961 gcatctgcat gtccagtatc gccacgtcgt agggatctcc gtcctcatct gcgatacgca
    10021 gcagttccag ggcgctctgc gcatcctcgg ccatcccgtt ttccatcccc caggagagga
    10081 tctgctggtg caagatcttg cggttggtct cgttgtcgtc aacgacaaac acgcgaaggt
    10141 caccgaggtc gggatgcggc tgccgaggac ggcctgccgc ctctggttgc atgtcgaagg
    10201 gcaactcgaa ccagaacgtg ctgccctccc ccggcgcgct ctcgacgccc atctctcccc
    10261 ccatcagctc taccagctgg ctggagatgg agagcccgag tccggtgccc ccgtactttc
    10321 tggtggttga ggcatcagcc tgggtgaaag atgtgaagag gcgcacctgc tgctcggacg
    10381 tcatgccgat gccgctgtcg gtaacggaga agcgcacaat ggcggagtcg tctctcgctt
    10441 ccgagagctc ggcccttacc acgacctcgc cctcctccgt gaacttgatg gcattgccta
    10501 tgaggttcgt cagcacctgg gtgagacggc cagggtcacc tctcaatacc tcaggcacgt
    10561 cgtaggtgac gagggtggcc agttccaggc ccttggcgtg agccccttcg acgaacaggt
    10621 cgagggcctc ctccaccgta ttgcgcaggt cgaagtctat gacctcgaac tccatcatcc
    10681 ctgcctcgat cttcgagaag tcaaggatgt cgttgatgat cgtcagcagg tgctcccccg
    10741 aagtccggat cgtccccgcg tactccagtt gctcttcgga gaggtcggtg tcaagaagaa
    10801 gacccgtcat gcctatgacg ccgttcatgg gggtccttat ctcgtgggac atgttcgcaa
    10861 ggaactcgct cttggccctg ttggccgctt ccgcagcctc cttggcactc ctcagctcat
    10921 ctcccgcttg ctctagctcg gtgacggcgc tctggagttg ctcggtgcgc cgctcgatac
    10981 gctcctcgag ctcctcgttg aggcgcctga tctcctcctc cgtgctcttg cgctccgtga
    11041 tgtcgcgcac gacgcccgac atgcgaacag ggttgccctg ctcgtcgcgc tgggcctctc
    11101 ccctgacaat gcacgtgcgg tactcacccg acggacgccg aacccggcat tccagggcgt
    11161 acgcctcgtc gcgctcgaag tgggcggtga tggcttcgat aactctatgc ctgtcctcgg
    11221 gatgcagatg atcctcgaaa agctccaggc tggggacgac ctcggaaggc gagaggccgg
    11281 cgatctcgta gaggcgctcg ttccagtaga gctcgccggt gcccaacttc cagtcgaaga
    11341 cgccgtcgtt ggagaccttg actacaaggg agtagcgctc ctcgctctcg acgagccttc
    11401 gctcgttctc ccgcagctcg gacactatgg tttcgagctg ctcggtgcgc cgctcgattc
    11461 gctcctcgag ctcctcgttg aggtgcctga tctcctcctc ggtccgcgtg agagcggtga
    11521 tgtcggtgat cttgctgacg tacagtctct ggcccgcacg ggtgacggcc ataacatcgg
    11581 ccgtcacaac ggctaactcg ccgccctttt tcctgtgccg cctctcctgg atgagcgctt
    11641 cgtccccgtg gcgcatacgc cgggcgtgat tccgctgtcc tgcatgctct tccgaaacgg
    11701 tgatgtccga tacattcatc ctcctgaatt cttctcgggc gtagccgtag aactcgcagg
    11761 cgtatgggtt tacgtcgatg aagttgaggt gttcgtcgag gaggaagata ggatggtgca
    11821 ccctctcgta gagcgcccgg aagaactcct cactctctct taacgcctcc tcggcccgct
    11881 tgcgctcgga tatgtcctgg tactggccaa cgaagtaggc gggatctcct ctcgagtccc
    11941 gcaccaacga gacgctcgag atagcccata cgatatcccc gtcggcacgg atgtagcgct
    12001 tctgcacgag gtccctcttg actttccctt ctaacagcac gcgcgtgcgc gtggtagtta
    12061 tctcgcggtc atcaggatgg gtgacctccc gcgacctctt cgagagcaac tcctcctccg
    12121 agtagccaag catgtcgcac aacgcctggt tcacttgcag gtaccggttg tcgaggctgg
    12181 tgagggacat gccgatggga gcgttctcga agatattgcg gaagcgtcct tcggtttcct
    12241 ctagctcaga ctcagcccgc ttgcgctcgg tcacatccat caacaggccg tcccatacga
    12301 tgtctccgtt ggccctgcgc tgcggacggg aggccttcct tagccacttc tgctcgcccg
    12361 agcgcaacct gactctgccc tcccactcac agggcgagag cgtggccgcg gactctgcca
    12421 gcgcgaggtc gaagctcggg cggtcttccg gatggatagc gtcgatgtag agggaggggt
    12481 cctgctggag ctcgtgcggc tcgaggccgt agatctcacg agatcccctg ctgacgaagg
    12541 gtagcgctat ggagccatcc tggcgcctga ccatctggta gaccatccca ggggcgttcg
    12601 ccacgatcct cgagtagcgt gcctcgctgt cttccaactg cgcggcctgg gcagagaacc
    12661 tccggtcgat aaacgcgctt atgagggcaa gccctagtat gagcagggtg aaggtgccga
    12721 tgccgaaacc catgaccgtg gtgttcaggt cgttggacgg ggccgccctt tcgctggtat
    12781 gcatgaaggt ggccgcggcc atccctgtgt agtgcatccc ggccacggcg gtgcccatga
    12841 cgagcgcgct gacagccttg agcagcgtac gcgtgcccct tgtcagatct cccccgttga
    12901 gccggagaaa gagccacagt gcggccaccg aagcgccgat ggcgatcaca aatgaggttg
    12961 cgacgaggag gagatcgtag cgcaccgtgg cctgcatcct catcgccgcc atacccgtgt
    13021 agtgcatcga ggctatgcct acccccatca agggtcctgc cagcgcaagc tcgcgtactc
    13081 cgacgacacc ccggctcacc acgaagaacg cgagagtagc ggcggcaatc gctacggcca
    13141 tggagagcag ggtgccgaga acgtcgtagg ttatcggcat gtccaacttc agggcgagca
    13201 tccccgtgaa gtgcatggac cagatcccca tgctcatagc gaaggctccg ccagcgagcc
    13261 acgcgatcct acccctgccg ccggttgccg tgacccgccc ggcgaggtcg agcgccgtgt
    13321 aggatgcaaa cgcggcgatc acatacgaga gcaccaccag acggtagtcc caacctacag
    13381 ccatagccgt ctccgcatgg ttcaagcttt gattcacctc ttacaacaat ccgccagatg
    13441 aggacagccc ctgaagcgcc acgctgggcg tcaacatcct cccagagcta gcaagtattg
    13501 tacatcaagc tagatccaat tcttgttcca cgaacgaggg ccgcggaaag gcgtcacgcg
    13561 gcgcacgaag ggatatccgc ccgcgatgcc tgctggtctg gtggctcgcg aagctctaac
    13621 atggccgcat ggatcacgat gaagtcggcc gctactggga cgagaacgcc gatgtctgga
    13681 cggagcttgt acgggccgga tacgaccact acagggacgg cctcaactca ccggccttcc
    13741 tcgacatgct tcccgacgta aatagtctct ccggcctcga cgtgggctgc ggagagggac
    13801 acaacaccag gttgctcgcg cagcgaggcg cccggattac cgcgatcgac atctccagaa
    13861 ccttcatacg ctacgccaga gaggccgagg aagggcaccc tcttggaatt cgctaccaga
    13921 ccgcgagcgc cgtggatctc ccgttcgatg aggcgagctt cgacttcgcc accgctttca
    13981 tgagcctaat ggatattccc gaaaccgggc gcgtgctcgc cgaagtcttc agggtgctga
    14041 agcctggcgg gttcttccag ttctccatca cccatccttg cttcgatacg ccgcaccgca
    14101 agaaccttcg cgatgagagt ggacgaacgt acgccatcga ggttggggac tactatcgca
    14161 ggcagagagg tgaggtagag gagtggatct ttagcgccgc tccacccgag gtcagggaag
    14221 gtttgcgccc tttccgcatg ccccgcttca cacgcacctt gagcgattgg ctgaacctgc
    14281 tggtagagac tggcttcatg ctggaacgtt tcggtgaacc gtatccgagc gacgaggctg
    14341 tcagggaacg tcccgggctg caggacgcac aggtcgtggc gtacttcctt cacgtcaggg
    14401 ccaggaagcc agcctagccg aagccggcac cttccaatgc gggtagcgcg gtccgcgcgt
    14461 agcgggcggc gtaacccgtg ccggcgcgct cagggaactc gcggggttcc ctggcctcga
    14521 actccctggc tccaaccccg gtgcggatgc ggcaatccgc gaagtctatc ctgatagggt
    14581 ctccatcccg gagccggctc aggacgccgc ccgcagctgc ttcgggggtg aatagagaga
    14641 cccaggctcc ccgagcgtct tccggcgcga gcccgtcggt caacacaggc acctccagtc
    14701 tcgcctcccg gagagcccgc cccagcgcgt cgagcctcaa tagtcctggt cctccgcgag
    14761 gcccgaagcc tccaaccacc agcatcgttc cgtcctcaac gtcgccccga cgtacgacaa
    14821 gaactgcctc ttcctcggaa ccgaagacgc ggcactcccc cgccacctcg gtcacgcccc
    14881 cgtgtacccg gcagacgccc tccgcgcccg acgccctggc catgacgaag acgagccgtg
    14941 agtgttcccc tggtggagga ggtaatgtgg gcagtttctc tttcaggttg cccgcgacgg
    15001 tcctgacgtc gtggagggag tcgcccaaag aggcgagcag gccagccacg ccgtgctccc
    15061 gcaaccagtc aggggcggcc tcaggagtct cggaggcgag gaccctgacc atctgggcga
    15121 atcccgcaac gccaccctca cgggcgatcg ccgcgagatg gaccagtatc tcaggtccac
    15181 cgcgggccgc gacgccggcc cgcagagcgt tggcgagaga gaaattctcg accagttccc
    15241 tgggacgcgg tccgtcgctc gcttcgacgg cggtgccggc tggttctccg gagatcgggg
    15301 cgagacccaa agcgacaagg gcggcggaga acggtttgcc tgatgctggg acgcagaccg
    15361 tcgggagatt taggcgcagc gcggccatta gcatccctgc gagatcctcg ggatggacgg
    15421 cgtctagcaa cagagcgtcg agatccatcc ccgagcagga gacctccacg acatcggccg
    15481 cccactcgcg cgccagcgcg acgccgccgt ccgggcgggc tggcggcagg cgtagcggga
    15541 ctggttctcc accggcatcg tggacggcgt cagggaggcc ggagggttcg tagaaaggga
    15601 cgccgatacg cggggggttc aggacggctg gcctctagga gcccagcttc tccataagga
    15661 gctggcggac ccgtccgccg tcggcgttgc cgcgggtcgc cttcatcacc tgcccgacga
    15721 ggaagccgac gaccttcttg tctccgtttc tgacccgctc ggcctcgtcc gggttggccg
    15781 agatcgcctc ctcgacgacg ccggagagct cgtccgagcc gaccgaagca agaccctcgc
    15841 gctctacgac ggcggacggg gaatccccgg tctcgaagac gcttgccagc acgtccttcg
    15901 cggcggagcg ggagaccttg ccggcggcga cgagttcgat caactctatc atgtgcccgg
    15961 acgttacctt ggaatcggag atatccgttc cggtctcatt gaggagggcc ctgaggtcgc
    16021 ctgagatcca gttcgcagac tgtttgggat ccacctcggc ggctactgcc tcgtagaaca
    16081 acgcgaggtc cctgtccgcc gtgagtacgc cggcatcgta ctcagagagg gcgtactgct
    16141 gcacgaatcg cgctcgcatc tggtcaggta gctcgggaag ccgctcccct atctcgcgga
    16201 cccgggccgg agacatctcg agcggaagga ggtccggctc ggggaagtat ctgtagtcgt
    16261 gtgcgtactc cttgctgcgt agctcgtgca cctcgtcggt ctccgggtcg tagtggagcg
    16321 tggtctgctc gaccctgcct ccgttctcta ggatctcacg ctggcgctcc agctcgcgcg
    16381 cgagaccgcg ctcgacgaag cggaaggagt tcatgttctt gagctcggtc ttggtgccga
    16441 atgagccgtc ggggttgcgt atggagacgt tggcatcgca gcgcagctgc cccttctcca
    16501 tgtcagcctc cgagacgccg acagcacgca atatctcctt gagctggttg gccgtcgcac
    16561 gtgcggcttc aggagatgga atgtccggtt ccgtgacgat ctccatgagg ggcgtgccgc
    16621 cgcggttgaa gtcgatgagg gagccgaccg agccgtgcat acggcccgag gtgccgacgt
    16681 gtacgttctt cgcggcgtct tcttcgaggt gaaggcgcgt gatgccgacc cggatcctgc
    16741 cttccgacgt aggcacgtcg atgtaaccgc caatacagat aggttcgtcg aactgggaga
    16801 tctggtaccc cttcggaagg tcggggtaga agtagttctt cctggcgaag acggccctcc
    16861 ctgcgacgtc gcagttgagc gcgagccccg ccatcacccc gagttcgacg gccttctcgt
    16921 tggtgacggg cagcgcccca ggatgcccga ggcacaccgt acaggtgtgg gtgttcggtg
    16981 cctcgccgta ggtcacgctg caaccacaga acatcttggt cctggtcgcg aggtggacgt
    17041 gaaactccag tcctatgacc gcctgatagg tacgcttctc ctgtacttcc aaatgccagc
    17101 tcccgtgcca gactgccgtt gtatcctcat tctagcatcg ccgggctccc ggcttgctct
    17161 gtggagcgaa tgctagaatg gctctggacg gaggttacgt cagcttgcat accgggatag
    17221 aaaacgactt cgccggggtg aaccggccac acctcgtggt cttgcaaggg ccgggacttg
    17281 gcgacttctt cgagatagac gacgacccgc tcgtactcgg gagcgaccct ttccgggcag
    17341 acatcgtggt gcgggacgtt gaggtcgagc cgcggcacgc cgaggtctac agagaaccgg
    17401 gcggctatgc cgtgcggggc gtaggagcca ggaagagctt cgcgctcaac ggtcgggtca
    17461 tagaggagct tgggggaggc cacctcctct ctgatgggga ccgcatccac ctgggtgact
    17521 ccgtcctcga gttctcgcac caggatgaga tcaaagcctc cttctacgaa cagttgcacc
    17581 atctcgtgaa ccgcgaccac cttaccggtc tctactccaa gggacgcttc gaccgcgagt
    17641 tcgagcatcg cctcgaagcg atggccgacg aagggaggcc acttggcgtc ctcatggctg
    17701 atatagacag cctgaagaag ataaacgacg cccacggaca cctgctcggc gagttcgtcg
    17761 tgggggagat cggccgcatc atcggcaccc tgcatgaaga cgagggacgc ctcgcgaccc
    17821 gattcggagg cgacgagtac cagactatcc tccccgatct ctccaaagag caagccatcg
    17881 aggtggcgga ggagatccgt cgccgcgtcg cctcgcatac cttcgaaagg ggcgatgtcg
    17941 ccgccagaac cacgatctcg ctgggcgtcg cgacctaccc ggaggacggg aaaacgcgcc
    18001 aggaactcac acacgccgcc gacgccgccc tctacagggc caaacgagcg ggcggcaaca
    18061 ccgtctccga gtaggcgccg ccggcaagaa gccaggcggc caggctggcg ctccaaacgc
    18121 ttgaacaggg catataatgt gcctgttgtg acgagatcgg catgtcggaa tggtttcggc
    18181 gtccgacctc gcgcgacggc gtatcgaggc ggctgaggca aaggaggatc tcatgaccca
    18241 gccacaaact gccctacccg ctgaagccgg tcactggtgg gcgctggcgc tgaggggtgt
    18301 catagccata ttgttcgggc tggctgcgct actgcgcccc gacatcgcgc
//
LOCUS       DATAIR010000063        11425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_280738, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000063 DATAIR010000000
VERSION     DATAIR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280738"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHM69_01645"
     CDS             complement(<1..593)
                     /locus_tag="VHM69_01645"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HEX2739122.1"
                     /translation="MGFLKHIEKRMESLVEGVFGRAFRRQIHPVEIAKGLTKQMDEGR
                     MVSISRTYAPNDFTVHLSKEDTESIQAYQASLKDELIQYASTHAENKNYHLMTPPRIH
                     FETEETLRFGEFGVTAKLTGGDGPREKGAPQDTSGQTRIFRTDESTGGEMDQGTSAIS
                     VDEAKRHGLAREIVEVVLADEKYPLEGHGPWSVGRSQ"
     gene            complement(600..2216)
                     /locus_tag="VHM69_01650"
     CDS             complement(600..2216)
                     /locus_tag="VHM69_01650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2739123.1"
                     /translation="MTETRYIEGQGRYALAEMIGRGGFATVWRARSLKGSLRGRDVAV
                     KVIPVYSTGERSRALREGQIAEGLRHKNIVETLEVIPGDHEIYLVTEYVKGMPLDEAA
                     KAYDVDEIVDSLTQILEALVYAHGQGIIHRDIKPQNALVDARGRVKLTDFGVAFRAGD
                     TRLTRVGFAVGTPGYIAPEILDGADPTALTDIYAVGATARTLLAHQPYEPPQRLREFV
                     DRTTSPNPAHRPQSAWAAVKLLTGRKAPSGSVSPAGLEGPSRLSEGFKDGALRLINGL
                     AAGWLGFLLGSQILDGAQAAGVAAGLGVLAYLLPRLGALGVIVALAVLLIRNGAGLGF
                     SALAPVLGGVWVMGVGSANGDVRRLPLGPALAVPLTLVAGLGSTLGAGLPLLFGALMR
                     PLGACLSAAASVLTLICYELTVGDGVFADGAPYAELPFTGAHLYAVPESFGIEQVFHR
                     AQLIVQDYPQLLLLIALWATMAGVVSLSEWAGKPFVGLAAAAGGGMLGYALLVSSKAP
                     PGTLSSDMISLGLVAIIYAVLRYLVSRVRG"
     gene            2523..2606
                     /locus_tag="VHM69_01655"
     tRNA            2523..2606
                     /locus_tag="VHM69_01655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2557..2559,aa:Leu,seq:cag)
     gene            2780..3709
                     /locus_tag="VHM69_01660"
     CDS             2780..3709
                     /locus_tag="VHM69_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX2739124.1"
                     /translation="MVFGFIFFVARGCVATQQSTEVRKYVTSADSLLSDSSNAGRGEL
                     QRILSSAGGDPEKLDEEALAQVTNRSESHYLQALRQEEIPPEFEDAHHYVVTALGIRS
                     EATERLEQAASGDASGFGGELATAIEDYRTSDSIIANYYIPAVKGSLEVAGQTSDQGY
                     LEEPQSFMDYGALGFDVAPVAGTGRSDPNALHGVQISAVKVAGKPLYQDGNVVLTGAD
                     EPIFAVTVGNGGEATETGIEVEVIVNTSAERQSRSKTIARLEPKKTTTVDVGGFIPGE
                     LDETARVTVEAGPVEYEKTTSNNTITGTVTFGM"
     gene            3719..3997
                     /locus_tag="VHM69_01665"
     CDS             3719..3997
                     /locus_tag="VHM69_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2739125.1"
                     /translation="MVTAIVLVRTERERVQEAAQAMLAIEGVTEAYSVTGPYDLVVMV
                     RIPDFESLAEIVPERIAQVPGVDRTETMVAFRAYSNYDLDRAWSLGFE"
     gene            complement(4033..4851)
                     /locus_tag="VHM69_01670"
     CDS             complement(4033..4851)
                     /locus_tag="VHM69_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739126.1"
                     /translation="MIEWLMGLSSPFRYVVYVAGTLLLFFVAVGVGAAVAVVVSRQPE
                     TVATRSGSSAESGTLEGTMLETTVNARTLEDTVIKPINKKKANPQESEDENSFVHRAT
                     DENSRGDYTYISDPSVNSNANAVVLVIPTADRGSPGGAAYNHNIGVWYEGVDKAKWAI
                     FNQDRTAVPAGATFEVVVPPASEKFVHRAEPINIVGNDTYLDDPLTNGNPDAVLSVTQ
                     NWNPGGGRGVYNNHAISVLYDKDKQKWAIYNRDGAPIPDGAAFNVAVSGDAKGS"
     gene            complement(5345..6472)
                     /locus_tag="VHM69_01675"
     CDS             complement(5345..6472)
                     /locus_tag="VHM69_01675"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2739127.1"
                     /translation="MPIEFKEGGSGYSLGVEEELHIVDATTGELVQKIEEIMSRLPKE
                     LCEFVSYELFQSVMEIKTPPCATAADAEKHLRELRSRVGSWAAACGASLASAGTHPFS
                     RYKDQKITDQERYRKVIRTLRWIAEREVIFGQHVHVAVPGPEEAIVAHNRLAEMSPLL
                     LALSANSPFWQGFDTGFESARVKIFESFPRAGLPPAFPDYEAFENYVDLMVESGAMED
                     YTYCWWDVRPHAKFGTIELRVLDSQTSLRYTVALTALTQCLVAEALEDGGQRVPYDTH
                     LAAENKWRASRHGMDAAFYDANEKTTVPARDITRDLIERLKSHAQDLACENELEGVLE
                     IVESGTGSQRQREVYEASGDFLDVVAFLIEGTRPALAEEQS"
     gene            complement(6499..7104)
                     /locus_tag="VHM69_01680"
     CDS             complement(6499..7104)
                     /locus_tag="VHM69_01680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2739128.1"
                     /translation="MARTTPSTKNRGKTAKKDSSGGNRTLGLVLAGVTALVIAAIAAV
                     AVFSTLGGGGTENFTPNDEGLIPVGEKVPPFTASTVDGGSVTVGGGESQATMLVFFAT
                     WCPHCQREAPVISELESQYEGLRVVMIGIDGEDNPGKVRDFVEKYDIESPAVYKPSLG
                     QKYGVSGYPTVYVLDGGDEVVGAHTGEAPRDVYEGWIEEAL"
     gene            7156..7674
                     /locus_tag="VHM69_01685"
     CDS             7156..7674
                     /locus_tag="VHM69_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX2739129.1"
                     /translation="MAKEGQNRVSTARRFLGGSYATLLSRIVLGGVFLVAGASKVLDP
                     GGLAASIRTYELPLPEWFVSLSAHALPLLEVLLGLYLLAGLFTRLSAWATNGLMVVFI
                     LALVQGALRGLQIDCGCFGASAAESNLWLDAARDFCLLALGLHVALAPTGRFSVDALL
                     RRVRSGSEPSEV"
     gene            complement(7616..8551)
                     /locus_tag="VHM69_01690"
     CDS             complement(7616..8551)
                     /locus_tag="VHM69_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2739130.1"
                     /translation="MQPNRDRILGASAVVAAATMWGTLGIFAKILYADGVSFESLVAF
                     RASVGWVAILFFVLATGGASSLRVSGRDLVFLVPLGLVGIGCFYLFYFYTVRESTVGT
                     AAILLYSSPAFVVVLARLFLKEALNAAKVLALALTTSGIFLVAGAYDPANLEVNPTVL
                     LTGLLSGLTYGLYSIFGRPVAGRLGPSVILSYALFFGAALLVVAALPTLDTLVGLSAG
                     SYALLLMLAVVHTTLAFALYTFGLGRLGAGRAAIVATVEPVVAGVLGTVLLGEDLTLP
                     KVVGGILVISGAALAQVRLRKARSPSGPGAAGRPR"
     gene            8542..8730
                     /locus_tag="VHM69_01695"
     CDS             8542..8730
                     /locus_tag="VHM69_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739131.1"
                     /translation="MAANVPFVSCPQAVFIRHLLHLAHEDDDAWLDVETDYGERIRQY
                     NDLPALEYARAVHREDDA"
     gene            8733..9824
                     /locus_tag="VHM69_01700"
     CDS             8733..9824
                     /locus_tag="VHM69_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX2739132.1"
                     /translation="MTARRSQATRVGWAVLVVGASLAGLLAQGIGLPAGWLVGPMLVA
                     IAVALVWEGHPTMPRWGRTTSLAVVGGVLASAFKPSVLPLIARHWLPVVLVICATLIL
                     SLGTGLLLSVFARLDRKTAALGALPGAASGMLAMSGPLGADPRLVALMQYTRVVVVVV
                     SATLVSRFGLVPGTTSRPQTLDQSLQTTSQGANLLVHGTIPTYALTALIAALGAWAGT
                     RLRLPAGALVGPLLIGVALEELGVLHLLWPPGVPEAAYLVIGLWVGLLFDGASVKRAG
                     RLFPLVLASAVGLVVVCTALAWVLSALTGIDGMTAYLATTPGGIDSVAIVALGSGADA
                     PLVLAVQMLRLLAVVLAGSLLGRWLAASG"
     gene            complement(9916..>11425)
                     /gene="mutL"
                     /locus_tag="VHM69_01705"
     CDS             complement(9916..>11425)
                     /gene="mutL"
                     /locus_tag="VHM69_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX2739133.1"
                     /translation="FRGEALPSIASVSSFSLTTSTGEGAATKVVVEGGAKAEVSRASH
                     PKGTTVLVDRLFYNVPARRAFLKGARAERAAIVEVVTHLSIAHPAVAFRLSEKGRDYL
                     SLPVAGGLMERLAQILGVAKARALRKVEYESGAFGVSGYAALPSLTEGSRAHQTISVN
                     GRFVRAENLHRGLDDAYRATVPSGRYPPVAIEITADPRRVDVNVHPTKQIVRFSEDRA
                     AREAVAAAVRGAIEWRPPAPPSTPRAAERTFTQDRFSGPGASRPPADYRVAEGRTPYT
                     TARDLREARERLSEASRPLAKESGPSEDLPERGALPQASPEELRVIGQLGAGYILVEE
                     PESLWVVDQHVAHERAILDRLNDSDSPATVQSLLVPEVVDLSPGEAELAAASLEELSV
                     YGFEAEPFGPMSVRVTAVISTLADRDVAGAFKDALSAIAGTEPGHRREDHILATIACH
                     SAVKMGDRLSQPEMEALIKSWLTSRLPATCPHGRSICYRIGLNEVARKLDRH"
BASE COUNT         1956 a         3664 c         3687 g         2118 t
ORIGIN      
        1 tcctgcgagc ggccaacgct ccacggacca tgtccctcca gcgggtactt ctcgtccgca
       61 agaacgacct cgacgatctc ccgcgccaga ccgtggcgct tcgcctcatc gaccgagatc
      121 gcggacgtac cctggtccat ctcaccgccg gtagactcat cggtgcggaa gatgcgggtc
      181 tggcccgagg tatcctgcgg ggcacccttc tcgcgcggac catcgccgcc agtgagtttc
      241 gcggtgacgc cgaactcccc gaagcggagc gtctcctcgg tctcgaagtg tatccttggc
      301 ggggtcatga ggtggtaatt cttgttctcg gcgtgggtag aggcgtactg gatcaactcg
      361 tccttcaacg aagcctggta ggcctggatg gactccgtgt cctccttgga gaggtgcacg
      421 gtgaagtcgt tgggggcgta ggtgcgcgag atcgagacca tccttccctc gtccatctgc
      481 ttggtcagac ccttggctat ctcgacgggg tggatctgcc gcctgaaggc ccgtccgaac
      541 acgccctcca ccagcgattc catccgcttt tcgatgtgtt tgagaaaacc caaggaaccc
      601 taccctctca ccctcgacac gaggtacctg agcaccgcat atattatagc cacgagcccg
      661 agcgaaatca tatctgaaga gagggtgccg ggaggggcct tcgaagagac cagaagcgcg
      721 tacccgagca tccccccgcc cgcggcggcg gccaggccca caaagggctt tcccgcccac
      781 tcagagagcg agacgacccc tgccatcgtc gcccacagcg cgatcagcaa cagcaactga
      841 gggtaatctt gcacgatcaa ctgggcgcgg tgaaacacct gttctatccc gaagctctcc
      901 ggtacggcgt agagatgggc accggtgaaa ggtagctcgg cgtagggagc gccgtccgcg
      961 aacaccccgt cgcctaccgt gagctcgtag cagatcagcg tgaggacgct ggccgccgct
     1021 gagaggcacg ccccaagggg gcgcatgagc gcaccgaaga ggagcggcag accggctccc
     1081 agggtggagc cgagtccagc gacgagggtg agcggcacgg cgagcgcggg ccctagcggt
     1141 agccttctca cgtccccgtt cgccgaaccg acgcccatca cccagacgcc cccgagcacc
     1201 ggtgccagcg cggagaaccc gaggcccgct ccgttacgta ttagcagaac ggcgagggcc
     1261 actatgaccc cgagggcgcc gaggcgcggc aagaggtagg cgaggacgcc gaggccagcg
     1321 gctaccccag ccgcctgcgc tccgtccagg atctgggagc cgaggagaaa tcctagccag
     1381 cccgcagcca ggccgttgat gaggcgcagc gccccgtcct tgaatccctc cgagagcctg
     1441 gaagggccct ccagtcctgc cggcgagacg ctgcccgagg gggctttcct gccggtgagt
     1501 agcttgacgg cggcccacgc gctctgcgga cggtgggccg ggttcggtga ggtcgtgcgg
     1561 tctacgaact ccctgaggcg ctggggcggc tcgtagggct ggtgggcgag gagggtgcgg
     1621 gccgtcgcgc cgacggcgta gatgtcagtg agcgcggtcg ggtcagcgcc gtccaggatc
     1681 tccggcgcaa tgtagccagg tgtccccacg gcgaagccta ccctcgtaag gcgcgtatcc
     1741 cctgccctga aggccacccc gaagtccgtc agcttcacgc gcccgcgggc gtccacgaga
     1801 gcgttctgag gctttatgtc acggtggata atgccctgcc cgtgggcgta gacgagggct
     1861 tccaggatct gggtgaggga gtcgacgatc tcgtccacgt cgtacgcctt ggcggcctcg
     1921 tctagcggca tccccttgac gtactccgtt acgaggtaga tctcgtggtc gccagggatg
     1981 acctccagcg tctccacgat attcttgtgc ctgagaccct cggcgatctg gccttcgcgc
     2041 aaggcccgcg agcgttctcc ggtgctgtag acggggatca ccttcacggc cacgtcgcgg
     2101 cccctgaggg agcccttgag agatctggcg cgccatacgg tcgcgaagcc gccgcgcccg
     2161 atcatctcag ccagcgcgta gcgtccctgt ccttcgatgt agcgcgtctc agtcacgacc
     2221 tcgcgctatt gtatagccta cgacaaacct ttgatacttg agcagtttgc gggagggatt
     2281 tccctcccgc aaactacggc atcaggtttc aagcatcagc tgtcagcttt cgtcctgccg
     2341 tggcgcgtgc cgtagagtga gccacacggc gttttgcaga aagatcgaag ccggccgccg
     2401 aggcgcacgg cttcgatctt tctgcaaaag gaagtgactg aaacctgtag ccttattagc
     2461 ctgggcgtag ccttcctgtt acaattcccc gaggttcagg acgtttcccg gagagggcct
     2521 acgggcgagt ggcggaattg gtagacgcgc taggttcagg gtctagtggc cgagaggccg
     2581 tgggggttcg agtcccccct cgcccacgac ataatcttgg gggtttcgta tagagcgcag
     2641 atctcacagg gcagccccga agtctcggcg tccgagaccg tcgggccgtc cgaggagacc
     2701 ccgcagccgt cccaagaagc gccgccagag gacgctcccc gcctggctca cccttgttag
     2761 ctccctcctc ctgcttatcg tggtcttcgg gtttatcttt ttcgtcgccc gcggctgcgt
     2821 ggcgacccag caatcgaccg aggtacgcaa gtacgtcacg agcgcggaca gcctcttgag
     2881 cgactcctcc aacgccggca gaggggagct ccagcgtatc ctgagcagtg ccggcgggga
     2941 ccctgagaag ctggacgagg aggcgctcgc acaggtgacg aaccgctcgg agagccacta
     3001 tctgcaggcg ttgaggcagg aggagatacc ccccgagttc gaggacgccc atcactacgt
     3061 cgtaacggct ctgggcataa ggtccgaggc cacagagcgg ctcgagcagg ccgcttccgg
     3121 ggacgcgagc gggtttggcg gggagctcgc gacggccatc gaggactaca ggacgagcga
     3181 cagcatcatc gcgaactact acatccctgc ggtcaagggc tccctggaag ttgccggcca
     3241 gacgagcgat cagggctacc tcgaagagcc gcagtcgttt atggattatg gggcgctcgg
     3301 tttcgatgtt gcccctgtgg ccggcacggg caggagcgat ccgaacgccc tgcacggggt
     3361 gcagatctcg gctgtcaaag tcgccgggaa acccctgtat caggacggaa acgttgtcct
     3421 gacaggggct gacgagccga tcttcgccgt gaccgtcgga aacggcgggg aggcgaccga
     3481 gacgggcatc gaggtcgagg tcatcgtcaa caccagcgcc gaacgccagt ccaggagcaa
     3541 gaccatagcg cgcctcgagc ccaagaagac gacaacggtc gacgtcggcg ggtttatccc
     3601 cggcgagctc gacgagaccg caagggtcac ggtcgaggca ggcccggtcg agtacgagaa
     3661 gaccacatcg aacaacacca tcacggggac ggttacgttt gggatgtaag gagcggacgt
     3721 ggtaacggcc atcgtgctcg tcaggacgga gagggaacgc gtgcaggagg cggcccaggc
     3781 catgctcgcc atagagggcg tcaccgaagc atactccgtg acaggtccat acgacctcgt
     3841 cgtcatggtg cgcatccccg acttcgagag cctcgccgag atcgtccccg agagaatagc
     3901 ccaggtcccc ggcgtcgacc gtaccgagac catggtcgcc ttccgcgcct actccaacta
     3961 cgatctcgac cgcgcctgga gcctggggtt tgaatagctg gtcagcacgc ctcggcccgc
     4021 ggccttgcta cctcacgaac ccttggcatc tcccgagaca gccacgttga aggcggctcc
     4081 gtctggtatc ggtgcgccgt ctcggttgta gattgcccac ttctgtttat ctttgtcgta
     4141 caggacgctt atagcatggt tgttatagac acccctgcca cctccagggt tccagttttg
     4201 cgtaaccgag agtacagcgt ccgggtttcc gttggttagc ggatcatcga ggtaagtatc
     4261 gttgcccacg atgttgatcg gttcggcgcg gtgaacgaac ttctcggatg ccggcgggac
     4321 gaccacctcg aaggtagccc ccgcgggcac cgccgtccga tcctggttga agatcgccca
     4381 tttcgctttg tccacgccct cgtaccacac gccgatgttg tggttgtagg cagcgcctcc
     4441 tgggctaccc cgatccgcag ttggtatgac gaggacgaca gcgttggcgt tactgttgac
     4501 gctcggatcg ctgatatacg tgtaatctcc acggctgttc tcgtccgtgg cgcggtggac
     4561 gaacgagttt tcgtcctctg actcttgggg gttggctttc ttcttgttga tgggctttat
     4621 cacggtatct tctagtgtcc tggcattgac ggttgtctcc aacatggtgc cttcgagagt
     4681 tccggactcg gcggaactcc ctgagcgagt cgcgactgtc tccggctgcc ggctgacgac
     4741 aaccgccacc gctgcgccca cgccgactgc cacgaaaaac agcaggagtg tgcccgcaac
     4801 gtatactacg tacctaaacg gggacgaaag ccccatcagc cattcgatca catctcggga
     4861 cggcgaatgg acccgttagt atttcgtaac gcgctcattg cctcctgtag agttgcgtgt
     4921 accgtcccta gcgccccaca gttcaagcat cgggcacggc ggcggccccc ttcgagtgtc
     4981 gtcacaagtg tgggatagcg gtgacgacac tgtacccgaa gcgggaagat cgcttcctgc
     5041 gccatcaggt tcccatttcc tctcgaccct tactcaagat tcagccgcct actcaagact
     5101 caagcaccat aactggtagt gggagcagat acatcggcaa catggcctat ttatttgggg
     5161 tcatgtatcc aattgcgtgg aaagaagtca gcctgttgag gaacctgcac gtcgcgtgag
     5221 cacgagaacc tcgtcgcagc tagcatggcc tcccttgcgg taggaggccc atcatggtgc
     5281 gcatgaaaat ccgcagcttc actccgctaa agactgcttc taccgccgtc aggcggcgtg
     5341 ctgactagct ttgctcttct gcgagggcgg ggcgtgtgcc ctctatgagg aaggcgacga
     5401 cgtctaggaa gtcgccgctc gcttcgtaga cctctcgctg gcgctggctg cctgtgccac
     5461 tttccacgat ttcgaggacg ccttcgagct cgttctcgca ggcgaggtcc tgggcgtggg
     5521 acttcagccg ctcgatgagg tcacgggtga tgtctctggc gggaaccgta gtcttctcgt
     5581 tggcgtcgta gaaggccgcg tccatgccgt gcctggaggc gcgccacttg ttctccgccg
     5641 cgaggtgcgt gtcgtaggga acgcgctggc ctccgtcctc gagggcctcg gcgacgaggc
     5701 actgggtcag ggcggtgagg gcgacggtgt acctcaggct cgtctgtgag tcgaggaccc
     5761 tgagctctat cgtcccgaac tttgcgtgcg ggcgtacgtc ccaccagcag taggtgtagt
     5821 cctccatcgc gcccgactcg accatcaggt cgacgtagtt ctcgaaagcc tcgtaatcag
     5881 ggaaggcggg ggggaggccg gcgcgcggga aggactcgaa gatcttcacc ctcgccgact
     5941 cgaagcccgt gtcgaaaccc tgccagaaag gcgagttcgc cgagagagca agcaggagag
     6001 gagacatctc cgcgagacgg ttgtgggcga cgatcgcctc ttccggtcct gggacggcaa
     6061 cgtggacgtg ctggccgaag ataacctccc tctccgctat ccagcgcagc gtccttatga
     6121 ccttcctgta gcgctcctgg tccgtgatct tctggtcttt gtagcgggag aaggggtggg
     6181 tccccgccga ggcgagcgag gcgccgcagg ccgcggccca ggacccgacc ctgctgcgca
     6241 gctcgcgcag gtgtttctcc gcatcggcgg ccgtggcgca cggtggagtt ttgatctcca
     6301 tgaccgactg gaacagttcg taggagacga actcgcacaa ctctttgggg aggcggctca
     6361 tgatctcctc gatcttctgg accaactccc ctgtggttgc gtcaacgatg tggagctcct
     6421 cctcgacccc gagggagtag cccgaaccgc cttccttgaa ctctataggc atggcgttcc
     6481 tcctctatcc tccggagatt agagggcctc ctcgatccaa ccctcataca cgtcccgcgg
     6541 ggcctcgccc gtatgcgcgc ccactacctc gtcgccgcca tccaacacgt ataccgtggg
     6601 ataacctgag acaccgtact tctggcccag cgacggcttg tagaccgcgg ggctctctat
     6661 atcgtatttc tcgacgaagt cgcgtacctt tcccggattg tcctcgccgt ctataccgat
     6721 catgaccacc cgcagccctt cgtactggct ctccagctcg cttatgacgg gagcctctct
     6781 ctggcagtga gggcaccagg tggcgaagaa gaccagcatc gtcgcctgcg attccccgcc
     6841 tcctactgtg acgctcccgc cgtcgaccgt actggccgtg aacggcggca ccttctcccc
     6901 gacagggatc agcccctcgt cgttcggcgt gaagttctcg gtacctccgc caccgagggt
     6961 gctgaacacg gcgacagcgg ctatggccgc gatgacgagc gccgtgacac cggcgaggac
     7021 gaggcctaga gttctgtttc caccggatga gtctttcttc gcggtcttcc cgcggttctt
     7081 ggtggacgga gttgtgcgag ccatgcgaac cctttgcgtt caacgtcgtt tacgtggggt
     7141 aacatagtac ccgctttggc gaaggaaggc caaaaccggg tctctacagc gcggcggttc
     7201 ctcgggggct cctacgcgac gttgctgtcg aggatcgtcc tcggcggcgt gttcctggtt
     7261 gccggggcta gcaaggtact cgaccccggc ggcctggcgg cttcgatccg cacctacgag
     7321 cttcccctgc ccgaatggtt cgtctccctc tccgcgcacg ccctgcctct cctcgaggtc
     7381 ctgctcggac tctatctgct cgccggattg ttcaccaggc tctcagcctg ggctacgaac
     7441 gggctcatgg tcgtattcat cctcgccctg gtgcagggag ccctgcgcgg cctgcagata
     7501 gattgcggct gcttcggtgc gtccgccgcc gagagcaacc tgtggctcga cgccgcgcgc
     7561 gacttctgcc tcctcgccct cgggctgcac gtcgcgctcg cccctaccgg gcgcttcagc
     7621 gtggacgccc tgctgcgccg ggtccgctcg gggagcgagc cttccgaagt ctgacctggg
     7681 ccagagccgc accggagatc acgagtatcc cgccgacgac tttagggagg gtaaggtcct
     7741 ccccgagtag cactgtccct agtacaccag ccacaaccgg ttccacggtg gctactatgg
     7801 ccgccctccc cgccccgagg cgcccgagcc cgaaggtata gagcgcgaac gcgagcgtgg
     7861 tgtgcaccac ggcgagcatg agtagcaggg cgtaggagcc tgccgaaagg ccgaccagcg
     7921 tgtcgagcgt agggagggcg gccacaacga gcagtgcggc cccgaagaac agggcgtagc
     7981 tgaggatgac agaagggccg agtcgacccg ccacggggcg gccgaagatc gagtacagcc
     8041 cgtaggtcag gcccgagagc aaccccgtga gcagcaccgt cgggttaacc tccaggttcg
     8101 cggggtcgta ggccccggcg accagaaaga tgccagaggt ggtcagcgcc agcgcgagca
     8161 ccttggccgc gttcagcgcc tccttcaaga acagccgcgc cagcaccacg acgaaagcgg
     8221 gcgagctgta gagcaggatc gcagccgtcc cgaccgtact ctccctgacc gtgtagaagt
     8281 aaaacaggta gaagcagccg atccccacga gcccgagggg aaccagaaat accaggtcgc
     8341 ggcccgatac ccggaggcta ctcgccccgc cggtggccag cacgaaaaat aatatggcca
     8401 cccacccgac cgaagcccgg aacgccacca gcgactcgaa agagacgcca tcggcgtaga
     8461 ggatcttggc gaagatcccc aacgttcccc acatggtggc cgccgcaact acggctgaag
     8521 ccccgagtat ccggtccctg tttggctgca aacgttccct tcgtctcgtg cccgcaagcg
     8581 gtatttatac ggcacctcct gcatcttgcc cacgaggatg acgacgcctg gcttgacgtg
     8641 gagacggact acggcgagcg catcaggcaa tacaatgacc tgccggccct tgagtacgca
     8701 agggcggtcc acagggaaga cgacgcttga gagtgacggc gaggagatca caggcgacgc
     8761 gtgtagggtg ggcggtcctg gtcgtgggtg cttcgctggc tggactgctc gcccaaggca
     8821 ttggtctgcc cgcggggtgg cttgtcggtc cgatgctcgt agcaatcgct gtggcgctcg
     8881 tctgggaggg acacccgacg atgccccgct ggggccggac aacttcgctc gcggtcgtag
     8941 gcggcgtgct cgcctcagcc ttcaagccct cggtcctgcc cttgatcgcc cgccactggt
     9001 tgcccgtggt cctcgtgatt tgcgccaccc tgatcctcag cttggggacg gggctgttgc
     9061 tctccgtatt tgcccgcctc gatcggaaga cggcggcttt gggggcgctg cctggtgcgg
     9121 cctccgggat gctcgccatg agcggccccc tgggcgccga cccgcgcctc gtcgccctga
     9181 tgcagtacac gcgcgtggtc gtggtcgtag tatcggccac gctggtgagt cggttcgggc
     9241 tggtgcctgg tacgacttca cgtccccaaa ctctggatca gagtttgcaa acgacctcgc
     9301 aaggggcgaa ccttctcgtc cacggtacta tcccgaccta cgccttgacg gcgctgatcg
     9361 cggctctcgg ggcatgggcc ggaactcgcc tcaggcttcc cgccggggca cttgttggcc
     9421 cgctgcttat cggggttgct ttggaggagc ttggcgtctt gcacctgctc tggcccccgg
     9481 gcgtgcctga ggcggcctac ctcgtcatcg ggctctgggt cgggctgctc ttcgatggtg
     9541 cctccgtaaa gcgggctgga aggctgtttc ctttggtgct tgcctccgcc gtgggtctgg
     9601 tggttgtgtg caccgctctg gcgtgggtac tcagcgcgct caccggcata gacgggatga
     9661 cggcctacct ggccacgacg cccggcggga tcgactcggt cgcgatcgtg gccctcggga
     9721 gcggcgccga cgcgccgctg gtactggctg tgcagatgct gcggcttctg gccgtggtgc
     9781 tggccggctc gctcctcggg cggtggttgg cggcctctgg gtgacgggcc agtatcctgg
     9841 acaggttttc caaccaggta ggtgccgacg gtggatcatt gagatcctca agcaccggaa
     9901 gagccggcct cttatctagt gacggtcgag cttgcgggct acctcgttca ggccgatacg
     9961 gtagcagatg gaccttccgt gcgggcaggt ggccgggaga cggctggtga gccagctctt
    10021 tatgagcgcc tccatctcgg gttgcgagag cctgtcgccc atcttcacgg cagagtggca
    10081 ggcgatggtg gcgaggatgt ggtcctcgcg tcgatggccc ggttcggtgc cagcgatagc
    10141 cgagagggcg tccttgaaag cgccagctac atccctgtcg gcgagagtgg agattacagc
    10201 cgtaaccctg accgacatcg gcccgaaggg ctcggcctcg aagccgtaga cggagagctc
    10261 ttcgaggctt gccgccgcca gctcggcctc tcctggcgag aggtccacga cctcggggac
    10321 gagcaggctc tgcaccgtag ccgggctgtc cgagtcgttg agcctgtcga ggatggcccg
    10381 ctcgtgggca acgtgctggt cgacgaccca caacgactca ggctcctcga cgaggatgta
    10441 gcccgccccg agctgcccga tgaccctgag ctcctcgggc gatgcctgtg gcagcgcgcc
    10501 ccgctcgggc aggtcctcgg acggcccgct ttccttcgca agcggcctgg aggcctcgga
    10561 gagacgttcg cgggcctccc tgaggtccct cgccgtcgtg tacggggtgc ggccctccgc
    10621 gacacgataa tccgcaggtg gacgcgaagc tccgggccct gagaagcgat cctgggtgaa
    10681 ggtcctttct gcggcgcgcg gggtggaggg aggagccggt ggacgccact ctattgcgcc
    10741 gcgtacggcg gcggcgacgg cttcgcgggc agccctatcc tccgagaagc gcacgatctg
    10801 ctttgtggga tggacgttca cgtccacgcg gcgggggtct gcggtgatct cgatagccac
    10861 aggaggatag cggccggatg gcactgtcgc cctgtacgcg tcgtcgaggc cgcggtggag
    10921 gttctccgcc ctgacgaagc ggccgttcac cgagatcgtc tggtgggctc tgctcccctc
    10981 cgtcaggctg ggaagggccg cgtagccaga gacgccgaaa gcccccgact cgtactcgac
    11041 ctttcgcagc gcgcgcgcct ttgcgacgcc gaggatctgc gccaggcgct ccatgaggcc
    11101 ccctgccacg ggcagcgaga ggtagtcgcg gcccttctcc gaaagacgga aggcgacggc
    11161 gggatgggcg atcgacaggt gtgtcacgac ctcgacgata gccgcccgct ccgcccgcgc
    11221 gcctttgagg aaagctcttc tggctgggac gttgtagaag aggcggtcga caaggacggt
    11281 cgtccctttc gggtgggagg ctctggagac ttctgccttg gccccacctt cgacgacgac
    11341 cttcgtggca gcgccctcgc cagtcgaggt agtcagggag aaagaggaga cgctcgcgat
    11401 cgagggcaag gcctcgcccc tgaat
//
LOCUS       DATAIR010000064         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_232413, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000064 DATAIR010000000
VERSION     DATAIR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232413"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHM69_01710"
     CDS             complement(<1..536)
                     /locus_tag="VHM69_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX2739134.1"
                     /translation="MNNADYTRYRLRPGKAFSLSTVDPGDTQGHPGKKRARKQLRANL
                     ERLSTLQERLYAESGGSVLIVVQAMDGGGKDSTIRRVFRGVNPQGCYVVNFKAPTPEE
                     LAHDFLWRVHRRVPGKGYIAVFNRSHYEDVLVARVRGLAPVELIEERYGHINSFEKLL
                     HDHGTRLIKVFLHISKEY"
     gene            complement(533..1594)
                     /locus_tag="VHM69_01715"
     CDS             complement(533..1594)
                     /locus_tag="VHM69_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739135.1"
                     /translation="MKTRYFFTSLTRISDLQKVPFSVEHLPRRGWETGDYVVGEVASP
                     PNRLSMIELGNGRMVEVVEGDRIVGAFGVRYATLEMVGGWQGIGHDQMMEALTGAGLF
                     GKSTSRSTLLPSPPSLLYKGHVLLGQKKATMRDYAPEVPERAFDLPVVLLVGTSMSAG
                     KTTSAKVIARLLREAGLVVVGAKLTGAGRYRDILAMQDAGADHIFDFVDAGLPSTVVS
                     EKEYRRALRNLLSRIAAVDADVVVAEVGASPLEPYNGATAIEEVGPNVRCTVLSASDP
                     YAVTGVVTAFGNRPDLVTGLATSTRAGIELVEKLCGIEAMNVLDRGSLPRLRSILDER
                     LDLRRLTDIQMVNWKEELS"
     gene            complement(1591..2757)
                     /locus_tag="VHM69_01720"
     CDS             complement(1591..2757)
                     /locus_tag="VHM69_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2739136.1"
                     /translation="MVETAQEVLGYLREQRLALVGFLERLALAESPSTEPRSQDEVLD
                     ILSEALTEIGYGVWRVPGRWSGGHLYARPEGRCAPAQLLLGHCDTVWPLGTLDMMPVE
                     VKDDVIKGPGVYDMKGGLAQMVFAIRALRDLGLRPNVTPIVFVNSDEEVGSRESTRHI
                     RRLARVVERAFVMEPSLGPSGKLKTARKGVGRYEVAVTGVAAHAGLNPEEGASAILEL
                     SYLIQALFALNDPARGTTVNVGTIDGGLGPNVVAPESSASVDVRVPTRGDARRVEETI
                     LGLKPVTPGVTLRVEGGMGRPPMERTPRNRRLWKAARGLGKELGLDLEEGMAGGGSDG
                     NTTSLFTATLDGLGAVGDGAHSRHEFVYTDRIVERCALLALLLMEPPCAGRSDP"
     gene            complement(2764..3372)
                     /locus_tag="VHM69_01725"
     CDS             complement(2764..3372)
                     /locus_tag="VHM69_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEX2739137.1"
                     /translation="MGEHIREGLVTISPDWPLWDRVFTVNPLVLVGTRERDGGYDFAP
                     KHMAFPMGWENYFGFVCTPRHATYHNARREGVFTVSYPKPTQVVLASLAATRREGDGS
                     KPSLYALPTFPASEVDGVFIEDAYLFLECVLDRIVDDFGENSLIAGRVVAAHVREDYL
                     RASDRDDNDLIHESQLLAYLSPGRYARVTESNSFPFPANFMR"
BASE COUNT          623 a         1156 c         1073 g          678 t
ORIGIN      
        1 tggtattcct tggagatgtg caggaatacc ttgatcagac gcgtgccgtg atcgtggagc
       61 agcttctcga acgagttgat gtgcccgtag cgctcctcga tgagctccac cggcgccaga
      121 ccacgaacgc gggccacgag cacgtcctcg tagtgggaac ggttgaagac agcgatgtac
      181 ccctttccag gtacacgcct gtggacccgc cagaggaagt cgtgggcgag ttcttccggc
      241 gtcggtgcct tgaagttgac cacgtaacag ccctgggggt tgacgcccct gaagacccgc
      301 cggatggtgc tgtccttgcc gccaccgtcc atggcctgca caacgatcag gaccgaacct
      361 ccagactcgg cgtagagccg ctcctgcaag gtggacagtc gctccaggtt cgctctcagt
      421 tgcttgcggg cacgtttctt gccgggatgg ccctgggtat ctccagggtc cacggtgctc
      481 agtgagaagg ctttgcctgg tcggaggcgg tatctggtgt agtcggcgtt gttcaagaaa
      541 gttcttcctt ccaattcacc atctgaatat ctgtcaggcg ccggaggtcc aacctctcgt
      601 cgaggatgga cctgagcctc ggcaacgagc cgcgatccag gacgttcata gcctcgatgc
      661 cgcagagctt ctcgacgagc tcgatgccag cccgggtgct cgtagcgagt cctgtcacca
      721 ggtcgggcct gttgccgaag gctgtgacga ccccggtgac ggcatagggg tccgaagcag
      781 acagtaccgt gcatcgtacg ttcggcccca cctcctcgat agccgtcgcg ccattgtagg
      841 gctcgagtgg agaggcgccc acctccgcca cgacgacatc ggcatcaacg gctgcaatgc
      901 gtgagagaag gttgcgcagg gcccgacggt attctttctc ggagaccacg gtcgaaggga
      961 gtcctgcgtc cacgaaatcg aagatgtgat cggcccccgc gtcctgcatg gcgaggatgt
     1021 ccctgtagcg ccctgcccct gtcagcttcg ccccgactac cacaaggccg gcctccctca
     1081 ggagacgcgc gatgaccttc gccgacgtgg tcttccctgc ggacatcgag gttccaacga
     1141 gcagcaccac aggcagatcg aacgcacgct ccgggacctc cggcgcgtag tcgcgcatcg
     1201 tcgccttctt ctgtccgagg agaacgtggc ccttgtagag cagggagggt ggcgagggga
     1261 ggagcgtgga cctcgatgtg gacttcccga agagccctgc tccggtcagt gcctccatca
     1321 tctggtcgtg ccctattccc tgccatcctc ccaccatctc cagggtggcg tagcggacac
     1381 cgaaggcccc tacgatcctg tctccctcca cgacctccac catgcgtccg ttgccaagct
     1441 ctatcatgga gagcctgttc gggggtgacg cgacctcccc cacgacgtag tcgcccgtct
     1501 cccacccccg cctgggcagg tgttccacgg agaaggggac cttctgaagg tcggagatac
     1561 gggtcaggga ggtaaagaag taccttgtct tcatgggtcg ctccttcccg cgcatggtgg
     1621 ttccatcaag agcagcgcca gcagggcgca tcgctcgacg atcctgtccg tgtagacgaa
     1681 ctcgtggcga gagtgggcac cgtcgccgac ggcccccaga ccgtccagag tggccgtaaa
     1741 gaggctggta gtgttgccgt ccgaaccccc accggccatg ccctcctcga gatccaggcc
     1801 aagttccttc ccaagtccgc gggccgcctt ccagagcctc ctgttgcggg gcgtacgctc
     1861 catcggagga cgacccatac cgccttccac gcgcaacgtc acgccaggcg tcacaggctt
     1921 caggccgagg atagtttcct ccacgcggcg ggcgtcccct cttgttggca cccgcacgtc
     1981 caccgaggca ctgctttccg gagcgacgac gttagggccg aggcccccgt ctattgtgcc
     2041 gacgttcacg gtcgtgccgc gcgccggatc attgagagcg aagagcgcct ggatcaggta
     2101 cgagagctcc aggatggcgc tggcaccctc ctcgggattg agtccagcat gcgccgccac
     2161 ccccgtgacg gccacctcgt agcgtcccac ccctttcctg gccgtcttga gctttccaga
     2221 cggcccgagc gagggctcca tcacgaacgc tctctcgaca acgcgcgcga gacgcctgat
     2281 gtggcgcgtg gactccctgc tgccgacctc ttcatctgag ttcacgaaga ctatcggggt
     2341 tacgttgggc ctcaagccga ggtcccgcag cgccctgatg gcgaagacca tctgcgccag
     2401 cccgcccttc atgtcgtaga cgccggggcc cttgataacg tcgtccttga cctcgaccgg
     2461 catcatatca agtgttccca gaggccagac ggtgtcgcag tgtccgagca ggagttgcgc
     2521 tggcgcacag cgcccctctg gcctcgcata gagatggccg ccgctccagc gccctggcac
     2581 gcgccacacc ccgtacccta tctcggtgag tgcctcggaa agtatgtcga gtacctcgtc
     2641 ttgcgaccgc ggctccgtcg agggtgactc ggcgagcgcg agacgttcca ggaacccgac
     2701 cagtgccagc cgctgttcgc ggagatagcc tagtacttcc tgtgctgtct ccaccatcct
     2761 ggactaccgc atgaagttcg ccgggaatgg gaacgagttg ctctccgtga cgcgtgcgta
     2821 gcgccccggg ctcaagtagg caagtagctg ggattcgtgg atcaggtcgt tatcgtccct
     2881 gtcagaggcg cgcaggtagt cctctctaac atgcgccgca acgacgcggc cggcgatcag
     2941 gctgttctcc ccgaagtcgt cgacgatgcg gtcgagcacg cattccagga acaggtaagc
     3001 gtcttcgatg aagaccccat cgacctcgct cgccgggaag gtcggaagcg cgtacaggct
     3061 tggcttggaa ccgtcaccct ctctacgtgt ggccgccaga ctcgcgagca cgacctgcgt
     3121 tggtttggga tagcttacgg tgaagacacc ctcgcgtctg gcgttgtggt aggtcgcgtg
     3181 gcgcggggtg cagacgaacc cgaagtagtt ttcccagccc atggggaacg ccatgtgctt
     3241 cggggcgaag tcgtaaccgc catccctctc cctggtcccg actagcacca gcgggttgac
     3301 ggtgaagacc cgatcccaga gaggccagtc cgggctgata gtgacgagac cttcgcgaat
     3361 gtgctctccc attttcttgc ctcctttaga gtcattatct gccccgcact cggcgtcaac
     3421 aagcgttccg gatctaacct gatcaatctt gatcagggaa ttgagcaagg gtgctcaggg
     3481 atcgctcgcc tcccggggtt acattcacac ctggacccgc cggaaggagg
//
LOCUS       DATAIR010000065         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_280792, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000065 DATAIR010000000
VERSION     DATAIR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280792"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHM69_01730"
     CDS             complement(<1..529)
                     /locus_tag="VHM69_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739138.1"
                     /translation="MEEVRRITKRRRRLRTVGEPHPWEPRHMRLAAMRFAESTGEPVE
                     RRNVTLGVIQHLWRSPVPFIGFYPPEGVKVTEHRLYSPAPPRLVDDTDYLVGVEAHKK
                     AFAKDLENAMVPAGRLAGYFEVPTDEGWRITLDLAQGRLGLADAWGMNSERRVVGDLA
                     PFAWAYRNCDFCIVTL"
     gene            complement(645..1346)
                     /locus_tag="VHM69_01735"
     CDS             complement(645..1346)
                     /locus_tag="VHM69_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX2739139.1"
                     /translation="MFIPGGFGGYILIMIVGMLISGAAALWVRSSYSKYSKQPSASGL
                     TGAQTARMILDRNGLTSVRVEPVAGQLTDHYDPRSKVVRLSEGNFRGNSIAGVSVAAH
                     EVGHAIQDATGYVPMKLRSGIFPVVNISSQFWPMAFIMAFFVGGAASPLGSLLINIAV
                     VLFIGVLLFHIVTLPVEINASTRAYGLLTRYGILSSTEAGGTRRVLTAAAFTYIAAAL
                     TAVLTFLYLLLVSRQ"
     gene            complement(1561..>2563)
                     /locus_tag="VHM69_01740"
     CDS             complement(1561..>2563)
                     /locus_tag="VHM69_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2739140.1"
                     /translation="YLVYGPLSNGATTVQFEGTPSYPGPDRHWDLIERHGVTIYYSAP
                     TVIRAFMKQGTEPLENHDLSSLRLLGTVGEPINPRAWEWYHENVGGGSCPIVDTWWQT
                     ETGAIMIAPLPGIVPTKPGSATRPFPGIFVDIYDEDDKPIEGPGKGNLVITKPWPGML
                     RTIYKDPERYRKTYWERLGDKYFVEDGAERDEDGYYTITGRIDDVMNVSGHRIGTMEV
                     ESALVSHEGVAESAVIGRHDEDKGQAIVAYVILEGDREGSDDLIKELNDQVRKAIGPH
                     ARPEEIIFTPDLPKTRSGKIMRRVLRGVAEGEDDLGDTSTLADPSVVDDLKEARQQS"
BASE COUNT          449 a          818 c          807 g          489 t
ORIGIN      
        1 agagggtgac gatacagaag tcgcagttgc ggtaggccca ggcgaagggt gccaggtccc
       61 caaccaccct cctctcggag ttcatgcccc aggcgtcggc cagccccagg cgtccctgcg
      121 ccaggtcgag cgttatgcgc cacccctcgt ccgtcgggac ctcgaagtat cctgccagcc
      181 tccctgccgg gaccatggcg ttctccaggt ccttcgcgaa ggccttcttg tgggcctcga
      241 ccccgaccag gtaatccgtg tcgtccacca acctgggggg agccggggaa tagaggcgat
      301 gctccgtaac cttcacgcct tcaggcggat agaacccgat aaacggtaca ggcgagcgcc
      361 agaggtgctg tatcaccccg agcgtcacgt tgcgccgctc cacaggctct ccagttgact
      421 cggcgaagcg catcgcggcc aggcgcatgt gcctgggctc ccacggatgt ggctctccca
      481 cggtccgtag cctccgccgg cgcttggtta tgcggcgaac ttcttccatg acgcgcgtat
      541 tctacaactc tcctgccgcc ggtgccgggc aaggggaaac ttgaggacag agcttgaggg
      601 cggggcaatt gccccgccct cgtggtcagg acgagagtac tctgctactg cctgctgaca
      661 aggagcaggt agaggaaggt cagcaccgcc gtcagggctg ccgcgatgta ggtgaaggcc
      721 gccgcggtca ggactcgcct ggtgccgccg gcctccgtgc tcgacagtat cccgtagcgc
      781 gtcaggagcc cgtaggcgcg ggtcgaggcg ttgatctcaa ccggcagcgt cacaatgtgg
      841 aacaggagca ccccgatgaa aagcacgacg gcgatgttga tcaggagcga cccgagaggg
      901 ctcgccgcgc ctccgacgaa gaaggccatg atgaacgcca tcggccagaa ctggctgctg
      961 atgttcacca cggggaagat ccctgacctg agcttcatcg gcacgtatcc cgtcgcgtcc
     1021 tggatagcgt gcccgacctc gtgagccgcc acactcaccc cggcgatgga gttgcccctg
     1081 aagttgccct cagagagcct gacgaccttg ctcctcgggt cgtagtggtc cgtgagctgt
     1141 cctgccacag gctcgacgcg gacgctggtc aggccgttgc ggtcgaggat catacgcgcc
     1201 gtctgggctc ctgtaagacc gctggcgctc ggctgcttcg aatacttcga gtaagaactc
     1261 ctcacccaca gggcggcggc cccggagatg agcatgccga cgatcattat gagaatgtaa
     1321 ccgccgaatc cacctggtat gaacaacgct atgcctccta gcaagtttac agcgcatatt
     1381 ttactacaca gacatacgca gagacgctcg aaaagttacg cgtgaaccac gcagagatcg
     1441 gggttactga ggaccggtgg cgtaccggat tgcccgctcg aaggcacgcg atgcgcggac
     1501 gagcgacggg gcgggatcta gtgaccccgc cccgactcat agaaccttaa ctatcgtgcg
     1561 tcagctctgc tggcgggcct ccttcaggtc gtcgacgacg gacgggtcgg cgagggtcga
     1621 ggtatcgccg agatcgtctt cgccctctgc cacgccgcgg agtacgcggc gcatgatctt
     1681 gccgctcctg gtcttcggga ggtcgggggt gaagatgatc tcctctggcc tcgcgtgcgg
     1741 acctatggcc ttcctgacct ggtcgttgag ctccttgatg aggtcgtcgg agccctctct
     1801 gtcgccctcg aggatcacgt aggcgacgat ggcctggcct ttgtcctcgt cgtggcgacc
     1861 gataacggcg gactctgcca cgccttcgtg ggagacgagc gcgctctcga cctccatcgt
     1921 gccgatgcgg tggcccgaga cgttcatcac gtcgtctatt ctgcctgtga tggtgtagta
     1981 accatcctca tcccgctctg cgccgtcctc gacgaagtat ttgtcgccga gcctctccca
     2041 gtaggtcttg cggtagcgct cggggtcctt gtagatcgtg cgtagcatgc ccggccaggg
     2101 cttggttatg accagattgc cctttccagg accctcgatg ggcttgtcgt cctcgtcgta
     2161 gatatcgacg aagatgccgg ggaatggtcg ggtggcgctg ccaggcttgg tcgggacgat
     2221 gccggggaga ggtgcgatca tgatcgcgcc cgtctccgtc tgccaccacg tatccacgat
     2281 gggacaactc ccgcctccga cgttctcgtg gtaccactcc cacgcccgcg ggttgatggg
     2341 ctcgccgacg gtaccgagca gccgcaacga cgagaggtcg tgattctcca gcggctcggt
     2401 gccctgcttc atgaacgccc ggatcacggt cggcgccgaa tagtagatcg ttacgccgtg
     2461 gcgttcgatc aaatcccagt gcctgtcagg cccagggtag ctgggcgtcc cctcgaactg
     2521 gacggtggtc gccccgttgc tcaaaggacc atagacgagg tac
//
LOCUS       DATAIR010000066         6463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_47842, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000066 DATAIR010000000
VERSION     DATAIR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47842"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="VHM69_01745"
     CDS             complement(<1..2356)
                     /locus_tag="VHM69_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2739141.1"
                     /translation="MNKSLRAEGGAWHTSEVAEVLQALGTDAGTGLDDKEAARRLEEL
                     GPNELEDRGTRSPWWILWEQFTSTMIVILLVAAVASALLGDYEDAIAIAVIVVLNAAL
                     GFAQEYRAERAMAALKQLSAPRVRVRREGHVREISARDLVRGDVVMLEAGNLVPADGR
                     LVEGVNLRVQEAALTGESEPVGKDPATLEEEDTPLGERAGMVYLSTVVAMGRGLFVVT
                     ETGMATELGKIAAMIQAASPEQTPLQRRLNEVGKVLALAALAIVAVVFALGLLRGVDL
                     EVMFLTAVSLAVAAVPEGLPAVVTIALALGAQRMLKRRALVRKLPAVETLGSVTVICS
                     DKTGTLTVNRMSATVLDVADRTVELGGGKSSAVADDPALALLLAGGALCNDALLEDDE
                     EDGFRAIGDPTEGALVVAAAREGMKKPDLEAALPRVGEVPFDSGRKRMTTVHEVIPDS
                     EIPEVLEPILKEGTPYIAISKGAMDSLLEISSEVWTGEGRTEPLDEGWRERILAANAR
                     LAGKGIRVLAVGFKRLWAFAGEEIEDDLTFFGMVGMVDPARPEARDAVETCKRAGIRP
                     VMITGDHPLTAGYIATELGIAAEGHIVTGRDLADLPVEELVDLVEEVPVYARVSPEHK
                     LAIVEALQERGHIVAMTGDGVNDAPALRKADIGVAMGITGTDVSKEAADMVLTDDNFA
                     TIVAAVEQGRVIYDNIRKFIKYLLTSNSAEILVMLVGPFLGLGLPLLPLQILWINLVT
                     DGPPALALSAEPAERDIMRRPPHPPDEGVFDRGLGRHVIWVGTLM"
     gene            complement(2663..3325)
                     /locus_tag="VHM69_01750"
     CDS             complement(2663..3325)
                     /locus_tag="VHM69_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739142.1"
                     /translation="MIPGRRQASGGAGLWLRWVLANAVGETIGLGGTALVAAAVFYAG
                     EGTGVYAAVALAGLAVMAGTLIEGTVVGTAQWLVLRRPLPGMSWRTWVLATGAGAFVA
                     WTLGMVPSTVLGSGGGEPAAEPGEATVLGLAFIMGLVLGPVLGLAQWLILRRFVKSAA
                     LWMPANALAWAFGMVVIFIGIDLALGGTSTVPILALTLAIAGAIVGAVHGLALVWFLR
                     SA"
     gene            complement(3333..3767)
                     /locus_tag="VHM69_01755"
     CDS             complement(3333..3767)
                     /locus_tag="VHM69_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2739143.1"
                     /translation="MNARRLYNPLVRWLLHTPLHVVMSGSVLLLTFKGRNSGNSYTTP
                     ISYVREGEDLLLVSSRDRSWWKNLRGGARVTLRIRGRDEEGVAEAFEGGAAEEGLLSV
                     MRAVPAYRRHWNVALGHHGRPEDPDDLARVAEGNVLIRVSRR"
     gene            complement(3764..5008)
                     /locus_tag="VHM69_01760"
     CDS             complement(3764..5008)
                     /locus_tag="VHM69_01760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2739144.1"
                     /translation="MLESVDVGKRSVADYETTAGPEAVERLRVRAEPLRGARILHLNA
                     TPYGGGVAEILRSEVPLLRDLGIAADWKIITGDKDFFSVTKTMHNALQGAKRSLTSQE
                     KEIYLTYSTRNARLLEEEYDLIVAHDPQPLAIPQLHGRGETKWAWRCHLDTSEPNENL
                     WDFLRPYLDDYDAAVFTMEEFAPPAFPVPRVEIIPPAIDPEGPKNIELPSRLAGRLLG
                     WLGVELDRPLVTQVSRFDPWKDQPGVIEAYRMVKEEVPDLKLALVGSMALDDPEGWEV
                     YRQIKEAARKDPDIDIFTNLTGVSNVEVNAFQRFSDVCVQKSIREGFGLVVSETLWKG
                     TPMVAGEAGGIPMQMPDGAGGFLVGSVEECAERLLYLLRNPEEGVELALRGKDIVKKR
                     FLLTRLISDEVSLYADLLGAAG"
     gene            complement(5036..5998)
                     /locus_tag="VHM69_01765"
     CDS             complement(5036..5998)
                     /locus_tag="VHM69_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2739145.1"
                     /translation="MFPTKILLAIDGSPHSELAARMAADFATKTGSELHVVHVGRVPS
                     AYAAAESEILDYEFWKEMREFAKREVSQSLEDEVRKIEGTGVKVEMAHVSVGRPEVEI
                     VRLAEEIGAGLVVVGSRGHGPLRRAVMGSVSEGVVRHAHGSVLVARGSGHDEGYKPGK
                     ILLALDGSKGGSVAERTAVEISTATGAELHIMRVLDTESYRPYPGPEFWEGWEEDLER
                     SELNVRAFLDEHASRLKADGVKVGEVHLALGEADREIVRFAEDVHAGLIVIGSRGLGG
                     FRRALLGSVSDSVVRHAHCPVMVVRQRQEREPVARQSEEGSAGP"
     gene            complement(6017..6280)
                     /locus_tag="VHM69_01770"
     CDS             complement(6017..6280)
                     /locus_tag="VHM69_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739146.1"
                     /translation="MARSADRSIGEHVRRAHEAIRGEHQLAEYVAGLEIEECARILAS
                     DPDGLPRLAALRDECRVGAAEKRSFERVIRTWTEAVLSGIEGG"
BASE COUNT         1087 a         2215 c         1923 g         1238 t
ORIGIN      
        1 ccatcaaagt cccgacccag atgacgtggc ggccaaggcc cctgtcgaag acgccctcgt
       61 cgggagggtg cggcgggcgg cgcatgatat cccgttcggc cggttccgcg ctcagggcca
      121 gggcgggcgg accatccgtg accaggttga tccacagtat ctgcagcggt aacagcggca
      181 gcccgaggcc gaggaatgga ccaacgagca taaccaggat ctcggccgag ttgctcgtaa
      241 ggaggtactt gatgaacttg cggatgttgt cgtagatgac ccttccctgc tcgacggcgg
      301 ccacgatggt cgcgaagttg tcgtcggtca ggaccatgtc cgcggcttcc ttggagacgt
      361 cggtgcccgt tatacccatc gcaacgccga tgtcggcctt cctcaaggca ggggcgtcgt
      421 ttaccccgtc gccggtcatg gcgacgatgt ggcccctctc ctgcagggcc tccacgatgg
      481 cgagcttgtg ctccggcgag actcgcgcgt agacaggcac ttcttcgacg aggtcaacga
      541 gctcctcgac cgggaggtcg gcgagatccc ttcccgtcac gatgtgaccc tctgctgcga
      601 tgccgagctc ggtcgcgatg tatccggcgg tcaggggatg gtcgcccgtg atcattacgg
      661 gtcggatgcc ggcccgtttg caggtttcca cggcgtccct ggcctcgggt ctggcagggt
      721 cgaccatacc gaccatgccg aagaaggtga ggtcgtcctc gatctcttcc ccggcgaacg
      781 cccatagacg cttgaagccc acggccagaa cgcggatgcc cttcccggcg agccgcgcgt
      841 tcgccgccag gatcctctct cgccatccct cgtccagagg ctcggttcgc ccttcgccgg
      901 tccagacctc gctcgagatc tccagcaggc tatccatggc acctttgctg atcgcgatat
      961 acggtgtacc ttctttcagg atgggttcga ggacctctgg gatctccgag tccggaatca
     1021 cttcgtgaac ggtggtcatc cgcttgcgcc cggagtcgaa ggggacttct ccgacgcggg
     1081 gaagcgcggc ctcgagatcg ggcttcttca taccctccct ggctgcggcc accacgaggg
     1141 ctccctcggt gggatctcct atcgcgcgaa atccgtcctc ctcgtcgtct tcgagcaggg
     1201 catcattaca cagggcgccg ccggcgagga gcagggcgag cgcagggtcg tcggccaccg
     1261 cggaagactt ccctccacca agctccacgg tacggtccgc cacatccagc acggtcgctg
     1321 acatacggtt cacggtcagg gtccccgtct tgtccgagca gatcacggta acagaaccca
     1381 gcgtctctac ggctggtagc ttgcggacca gggctcgcct ctttagcatc ctctgggcgc
     1441 cgagggcgag ggctatggtc accacagcag gcagcccctc gggcacggcc gccacagcca
     1501 ggctcacggc ggtcaggaac atgacttcga ggtccacccc gcgtagcagg ccgagggcga
     1561 agacaacggc gacgatggcg agggccgcga gggctagcac ctttcccacc tcgttcaggc
     1621 gccgctgcag gggggtctgc tccgggcttg cggcctggat catagccgcg atctttccaa
     1681 gctccgtcgc cattccggtc tcggtgacaa cgaacagacc ccttcccatt gcgaccaccg
     1741 tgctcagata gaccatgcct gctcgctccc caagcggcgt gtcttcttcc tccagggtcg
     1801 cagggtcctt ccccacgggc tccgattccc ccgtgagcgc cgcttcctgc acccgcaggt
     1861 tcaccccctc aacgagcctc ccgtcggcgg gcacgaggtt gcccgcctcg agcatcacca
     1921 cgtccccccg caccagatct ctggccgaga tctcccttac gtggccctcc ctgcgtaccc
     1981 tcaccctggg cgcggagagt tgcttcaggg cggccatggc tctctcggcc ctgtactcct
     2041 gggcgaagcc aagggcagcg ttgagcacga ctatgaccgc gatggctatc gcatcctcat
     2101 agtccccgag gagggcggag gccacagccg caacaagcag aataacaatc atggtggatg
     2161 tgaactgctc ccacaggatc caccagggac tccttgtacc cctgtcctcg agttcgttgg
     2221 gacccagctc ttcgaggcga cgggctgcct ctttgtcgtc cagccccgtc cccgcatcgg
     2281 tgccaagagc ctgcagcacc tccgcaactt cggaggtatg ccatgcgcct ccctcggcac
     2341 gcaggctctt attcattctc cgggtgcctt cgccgcgttt cgccggtctt tcggtgccac
     2401 ttacacattt ccagggcgac gcggcttgtg gtccttacgt tttctgtcat ggtacaggta
     2461 tcgtaagtgc agatcttacc ctccgggctc tttccatgag gcgcatgtcc gtcaggaacg
     2521 ctgcgacgac ggtggacgat cggggaccgc cgatgtgccc ggcctccgcg cgacgttccg
     2581 atgcctcccc cataagatac aggataacct tgttggagaa gccgggtctg aatatcggat
     2641 ctctcacgtc gtccggggtg gttcacgcgg aacgcagaaa ccacacgagt gcgaggccgt
     2701 gtaccgcgcc gactatcgct cctgcgatgg ccagtgtcaa agcgaggatc ggtacggtgc
     2761 tcgtgccgcc cagagcgagg tctatcccga tgaagatgac gaccatcccg aatgcccacg
     2821 cgagggcgtt ggccggcatc cagagcgcgg cactctttac gaaccggcgc agtattagcc
     2881 attgcgcgag gccgaggacg ggcccgagca cgagtcccat gatgaaggca agcccgagga
     2941 ccgtcgcttc gcccggttcc gcggcaggtt cgcctcctcc tgagccgagg acggtgctcg
     3001 ggaccatgcc cagagtccag gccacgaaag cccccgcgcc ggtcgcgagg acccaagttc
     3061 gccagctcat ccctggcagc ggtctcctca agaccaacca ctgggcggtg ccgaccaccg
     3121 ttccctctat caaggttccg gccatgacgg ccagccccgc aagggcaacg gcagcgtata
     3181 cgccggttcc ctcgcctgca tagaacaccg ccgccgcgac gagcgcggtg ccgcccaggc
     3241 cgatggtctc acccacggcg ttcgccagaa cccaccggag ccagagtccg gcaccaccgc
     3301 ttgcctgtct gcggccaggg atcatgcgcc tctcacctcc gcgagaccct gatcagtacg
     3361 tttccctccg ccacgcgcgc gaggtcgtct ggatcttccg gtctcccgtg atggccgagc
     3421 gcgacgttcc agtgacgcct gtaggcgggc acggcccgca ttaccgagag gagcccttcc
     3481 tcggcggctc cgccctcgaa tgcctcggcc acaccttcct cgtctctacc acgaatgcgt
     3541 agcgttaccc gcgcaccgcc tcgaaggttc ttccaccagg agcgatcgcg gctgcttacg
     3601 agcagcaaat cctcgccttc tcgcacgtaa ctgatcgggg tggtgtagct attgccgctg
     3661 tttcggccct tgaacgtcag gagcaggaca gatccactca tcacgacgtg cagcggagtg
     3721 tgcaggagcc atctcacgag tgggttgtag agtctccggg cgttcaaccg gcggccccga
     3781 gcaggtcggc atagagggag acctcgtcgg agatcagacg cgtcagcagg aaacgcttct
     3841 tcacgatgtc ctttccccgc agggcgagct cgacaccctc ttcaggatta cggagcagat
     3901 acaggagcct ctcggcgcat tcctcgacgg agcctacgag gaagccccct gctccgtccg
     3961 gcatctgcat cgggattcca ccggcttcgc ccgcaaccat cggggttccc ttccacaggg
     4021 tctccgagac gacgagaccg aagccctcgc ggatcgactt ctggacgcac acgtcggaga
     4081 agcgctggaa ggcgttcacc tccacgttgc tcacccctgt caggtttgtg aagatgtcta
     4141 tgtccggatc tttcctcgcc gcttccttga tctgacggta cacttcccag ccttcggggt
     4201 cgtcgagggc catggacccg actagtgcta acttgaggtc gggaacctcc tccttgacca
     4261 tcctgtacgc ttctatgaca cctggttgat ccttccaggg gtcgaagcgc gagacctgcg
     4321 tgacgagcgg cctgtcgagc tcgaccccga gccagccgag aagacggccg gccagcctgg
     4381 aaggcagctc gatgttcttc gggccttcgg ggtctatggc gggagggatg atctccaccc
     4441 gcgggacggg gaacgccgga ggtgcgaact cctccatggt gaagaccgcg gcatcgtagt
     4501 cgtcgaggta gggcctgaga aagtcccaga ggttctcgtt gggctcggag gtgtccaggt
     4561 ggcagcgcca tgcccatttt gtctccccac gcccgtgtaa ctggggtatc gccaggggct
     4621 gggggtcgtg ggcaacgatt aggtcgtact cctcctctag caggcgggcg ttgcgggtcg
     4681 agtaggttag gtagatctcc ttctcctgac tggtaagaga gcgcttcgcg ccctgaagag
     4741 cgttgtgcat cgtcttggtg accgagaaga agtccttgtc tcccgtgatg atcttccagt
     4801 ccgcggcgat ccccagatcc ctcagcagtg gcacctcaga gcgaaggatc tccgccacgc
     4861 cacccccgta tggcgttgca ttcaggtgca gaatacgcgc ccctcggagt ggctcggccc
     4921 tgaccctgag ccgttcgacc gcctcggggc ccgctgtggt ctcgtagtcg gcgacggacc
     4981 gtttgcctac gtctacgctc tctaacattc gctctccccg cgtccgccaa tgtccttacg
     5041 gtcctgcgct cccttcctcg ctctgcctgg ccacgggctc gcgctcttgc ctctggcgca
     5101 cgaccatgac cgggcagtgg gcgtgcctga ccaccgaatc ggagacgctg ccaagcaagg
     5161 ccctcctgaa gcccccgagc ccccgacttc caatgacgat caggccggcg tgtacgtctt
     5221 cggcgaacct cactatctcc ctgtccgcct ccccgagcgc gaggtggacc tcgcctacct
     5281 tcacaccatc ggctttcagc cggctcgcgt gttcgtccag aaacgccctg acgttaagct
     5341 ctgagcgctc gagatcctcc tcccatcctt cccagaactc gggccctggg taaggccggt
     5401 aggattctgt gtccaggacg cgcatgatgt ggagctcggc gcccgtggcg gtagagatct
     5461 cgaccgcggt ccgctcggcc acgctacccc ccttggaccc gtcgagggcg aggaggatct
     5521 ttccgggctt gtagccctcg tcgtggccgc taccgcgcgc aacgagcacg gagccgtgtg
     5581 cgtgcctgac gacgccttca gagacactgc ccatgaccgc gcgtctgagg ggaccgtggc
     5641 cccggctccc aacgaccacc aggccagccc cgatctcctc ggcgaggcgg acgatctcga
     5701 cctcaggacg gcccaccgag acgtgggcca tctcgacctt tacgcctgtc ccctctatct
     5761 tgcgtacttc gtcttcgaga ctctgtgaga cctctctctt ggcgaactct cgcatctctt
     5821 tccagaactc gtaatcgagg atctcggact ccgcggcggc gtaggcactg ggcacgcggc
     5881 caacgtggac cacgtgcagc tccgagcccg tcttcgtcgc gaagtccgcc gccatcctgg
     5941 ccgccagctc cgaatgcgga ctaccgtcaa tcgccagcag aatcttcgtc gggaacatgg
     6001 cgtacccctt tctacctcag ccgccctcga tgccagagag cacggcctcc gtccaggttc
     6061 ggatcacccg ttcaaaggac ctcttctctg ccgcaccgac tcggcactcg tcccttagcg
     6121 cggcaaggcg agggaggccg tcaggatcgg aagccagtat cctcgcgcat tcttctatct
     6181 ccagacctgc gacgtactca gcgagctggt gttccccccg aatggcctcg tgcgctcttc
     6241 tgacgtgctc gccgatggaa cggtctgccg atcttgccat gccggtactc cctccgttca
     6301 tcgcttgcct gcgttgacag tgtagctccc gagaatggac gtgacttgag caaattggct
     6361 catcttcatg gtgcgcattg cccaaaaggg aagggcgaag ttacgcactt cagataggtt
     6421 gaatcgtcat ggatcatgcg ggagctcgaa cgagccggta atc
//
LOCUS       DATAIR010000067         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_372875, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000067 DATAIR010000000
VERSION     DATAIR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372875"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..678)
                     /gene="coaE"
                     /locus_tag="VHM69_01775"
     CDS             complement(61..678)
                     /gene="coaE"
                     /locus_tag="VHM69_01775"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2739147.1"
                     /translation="MEGPVSVAVTGPFASGKSTLVRFLGEVPGVETASSDEMVHHLLQ
                     NEEQAISRVIDRFGEDVRGAGGIDRKALGRMVFGDAEALRDLEEILHPLVRRETVRRI
                     EASGADVFVVEIPLLFETERSEDFDYTVAVVVPEERRRAWSEERGVGEAALRAIEGRQ
                     LSGEEKARQADLVVQNDADLDKLKERAQELGRMISRERGSNVDPR"
     gene            complement(705..1688)
                     /gene="rpsA"
                     /locus_tag="VHM69_01780"
     CDS             complement(705..1688)
                     /gene="rpsA"
                     /locus_tag="VHM69_01780"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX2739148.1"
                     /translation="MMQKEDADGRLILSAKRAAFERAWNRIEEAYNDQRTVEGPVIEV
                     VKGGLILDIGLRGFLPASLVDIRRVRNLDSFLGQKLECKVIELNRSRNNVVLSRRAVL
                     EEERKEERERILTSLEEGMVVKGTVSNLVDFGAFVDLEGIDGLIHISELSWQHVDHPS
                     EVVEVGEEVEVKVLEVDRDRERISLGLKQTRKDPWQEIVERVKVGEQIKGRVTKLVSF
                     GAFVEVAEGVEGLIHISELADHHVETPDEIVRSGDEVDARIIDVDARRRRLSLSLRPK
                     REDREERPRREERPRREPEQRGTSDRRSSSEGSGLRSGAFDALADLDLEER"
     gene            complement(1704..>1955)
                     /locus_tag="VHM69_01785"
     CDS             complement(1704..>1955)
                     /locus_tag="VHM69_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2739149.1"
                     /translation="TTQKPSRELTMKDFFREENGEVIPLDESVLIDFKDGDIVEGEVV
                     RIDRDEVLVDIGYKSEGLIPSNELSIRKGADPHDVVDLG"
BASE COUNT          312 a          671 c          579 g          393 t
ORIGIN      
        1 gccagcaacg cgaagaagcc gcgtcgccgg cgggtccgcg acctgccgta cctgcgtcgc
       61 ttatcgtggg tcgacgttgg atcctctctc ccgcgagatc atgcgcccga gttcctgtgc
      121 ccgctcttta agtttatcca ggtctgcgtc attttgtacc accaggtcgg cctgcctggc
      181 cttttcttcc cctgaaagct gccgtccctc tatggcacgc agcgccgcct cacccacgcc
      241 ccgctcctca gaccacgccc gccggcgctc ttccggcacc acgacggcca ccgtgtagtc
      301 gaagtcctcg ctccgctccg tctcgaacaa caacggtatc tcgacgacga acacatcggc
      361 gccggacgcc tcgatgcgcc ggacggtctc gcgtcgtacc aggggatgca gtatctcttc
      421 gaggtcccgc agggcttccg cgtcaccgaa gaccatacgg ccaagagcct tgcggtcgat
      481 gccgcctgcg ccgcggacat cctctccaaa gcggtcgatg accctggaga tcgcctgctc
      541 ctcgttctgg aggaggtggt gcaccatctc atccgacgaa gccgtctcca cgccgggcac
      601 ctctccaagg aagcggacca gcgtgctctt gcccgacgcg aaaggaccgg tgacggcgac
      661 gctcaccggc ccttccatgg atcttctctg tgaaaagtcc tgtgctatct ctcctcgagg
      721 tccaggtcgg caagcgcgtc gaaggcaccg gagcggaggc cgctcccttc gctgctgctt
      781 ctgcggtcgg aggtgccgcg ctgctcgggt tcgcggcgcg ggcgctcctc gcggcgggga
      841 cgctcctcgc ggtcctcgcg cttcgggcga aggctcaggg agagcctgcg gcggcgggcg
      901 tccacgtcga tgatgcgggc gtcaacctca tcgccggagc gcacgatctc gtcaggggtc
      961 tccacgtgat ggtcggcaag ctcggagatg tggatcaaac cctcaacacc ctctgcgacc
     1021 tcgacgaagg ccccgaaaga aaccagcttg gttacgcggc ccttgatctg ctccccgacc
     1081 ttgacccgct cgacgatctc ctgccagggg tctttcctgg tttgcttgag gcctagagag
     1141 atgcgctcgc ggtcgcgatc tacctccagt accttgacct cgacctcctc gcctacctcg
     1201 acgacctcgc tcggatggtc gacgtgctgc caactgagtt cggagatgtg gatcagaccg
     1261 tcaatacctt cgagatccac gaaggccccg aagtcaacga ggttggagac ggtgcccttg
     1321 acgaccatac cctcttcgag tgaggtcagg atgcgctcgc gctcctcctt gcgctcctct
     1381 tcgagcacgg cgcgcctgct gagaacgacg ttgttgcggc tacgattgag ctcgatgact
     1441 ttgcattcga gcttctggcc gaggaaagag tcgaggttcc tgacgcgcct gatgtccaca
     1501 aggctcgcgg gcaggaagcc gcgcaacccg atgtccagga tcagaccgcc cttcactacc
     1561 tcgatgacgg gcccctcgac ggtgcgctgg tcgttgtacg cttcctcgat gcggttccag
     1621 gcccgctcga acgcggcccg cttggcagag aggatcagac gcccgtcagc gtcctctttc
     1681 tgcatcacga gggcttctag cctctagccg aggtcgacga cgtcgtgggg gtctgcgccc
     1741 ttgcggatgc tgagctcgtt ggaggggatc aggccctccg acttgtagcc gatgtcgacg
     1801 agtacctcgt ccctatctat ccggacgacc tcgccctcga cgatgtcccc gtccttgaaa
     1861 tctatgagga cgctctcgtc gagggggatg acctccccgt tctcctcccg gaagaagtcc
     1921 ttcatggtca actctcggga cggcttttgt gtggt
//
LOCUS       DATAIR010000068         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_186467, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000068 DATAIR010000000
VERSION     DATAIR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186467"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VHM69_01790"
     CDS             <1..660
                     /locus_tag="VHM69_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX2739150.1"
                     /translation="APLGATVESQKRLEALMDFTELGSGFAIAMRDLEIRGAGNLLGA
                     EQSGHIAAVGFEMYLTLLEEAVAAAKGEPEERREERPVIVELTLDAYLPPGYVMDEIE
                     RVDLYRRASGLGSLAEVEDLAEELVDRFGGLPEPARNLLGLSRVKLLARELGASSVTY
                     RSGNLTITGVTAGSAAPALIRRATGGTVTTRTGRTSVRAPELDPLTLAEESLRALVRS
                     G"
     gene            729..1745
                     /locus_tag="VHM69_01795"
     CDS             729..1745
                     /locus_tag="VHM69_01795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2739151.1"
                     /translation="MSKLLVNRMLFVIFLLVVALLVSLGCKPAEPQANVDSEAKKVAT
                     FEGGDVTQGELDEFAKQSGFGELSKDDPQYQTAIQQIMPQLVGIEITKAYAQEQNITV
                     SDEEVDKEIEKIKEQVGEQARSSGQDLSDQKAYEQALKQNDITEDELREDIRENLPVQ
                     KVQERVAGGAEPTDEEIQKYYEQNKEAQFTTPEQRCVRHILFNKDQKQKAEEVKQQLE
                     NGGDFAALAKEYSQDPGSAEKGGDLGCLGKGETVPEFEKAAFGAEQGEIVGPVQTEFG
                     YHLLEVTDIKPMQARSLDEVESQIRSQLSTEQQSEEFNKWLEEQKKKRDVKYLEGYKP
                     PDQG"
     gene            1851..>3128
                     /gene="eno"
                     /locus_tag="VHM69_01800"
     CDS             1851..>3128
                     /gene="eno"
                     /locus_tag="VHM69_01800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2739152.1"
                     /translation="MTEIVAVHGREILDSRGNPTVEVELATVSGFVGRASVPSGASTG
                     QNEAVELRDGDKDRYGGKGVLTAATNVDTELAQVVVGLDAADQRTLDLAMIEADGTEN
                     KGRLGANAILGVSLAAAKAAAEASALPLYRYLGGAGAYTLPVPCANIMNGGAHAANNV
                     DFQEFMVVPVGFDTYSEALRSVAEVYQVLKKLLAERGLSGGIGDEGGFAPDLESNGAA
                     LGLISEAVERSGYSLGDQMAFALDPAASEFYEDGTYDLAGEGKKLSREEMVDYYVGLC
                     DEYPILSIEDGLAEDDWEGWAIITGKLGDRVQLVGDDLFVTNPKILARGIDEGVGNSI
                     LIKVNQIGTLTETFETMELAHKSGYTTMVSHRSGETEDATISDLAVAVNAGQIKTGAP
                     ARGERVAKYNQLLRIEEALGTSAFYPGRAAFNPR"
BASE COUNT          660 a          933 c         1051 g          484 t
ORIGIN      
        1 gcccctttgg gtgcgaccgt cgagtcacag aaacggcttg aagcactgat ggacttcacg
       61 gagctcggga gcgggttcgc catcgccatg cgggacctgg agatccgggg cgcgggaaac
      121 ctgctcggcg ctgagcagtc agggcacatc gcagccgtcg gcttcgagat gtacctgact
      181 ctactggagg aagccgtcgc ggccgccaaa ggcgagcccg aggagcgtcg cgaggagcgg
      241 cccgttatcg ttgagctcac gctcgacgcg tatctgccgc cgggatacgt catggacgag
      301 atcgaacgcg tcgacctcta ccgcagggcc tcggggcttg gctctctagc cgaggtcgag
      361 gacctggcag aggagctcgt cgaccgcttc gggggacttc ccgagcctgc caggaacctg
      421 ctcgggctct cgcgggtcaa gctcctggca cgcgagctgg gggcaagctc cgttacctac
      481 cgttccggaa acctgacgat cacaggcgtc acggcgggct ccgctgctcc tgccctcata
      541 cgaagggcca cggggggtac cgtaacgacg aggacgggca ggacatcggt tcgcgcccct
      601 gagctggatc ccctcaccct ggcggaagag tcgctgcggg cgctcgtgcg atccggctga
      661 atccggaatc ccccgtgcta taatcgctgg cggtttctgg cattcaaaca gcgaggttct
      721 tcgtcgtctt gagcaagctt ttagtgaata ggatgttgtt tgtcattttt ctcctcgtgg
      781 tcgccctact cgtctccctt ggttgcaaac cggccgagcc gcaagccaac gtcgactccg
      841 aggccaagaa ggtcgccacc ttcgagggcg gcgatgtaac ccagggggag ctggatgagt
      901 tcgccaagca gagcggtttc ggagagctgt ccaaggacga tccgcagtac cagaccgcaa
      961 tacagcagat catgccccag ctcgtcggca tagagataac caaggcctac gcccaggagc
     1021 aaaacataac ggtctcggac gaagaggtag acaaggagat cgagaagatc aaagagcagg
     1081 tcggtgagca ggcccgctcc tcggggcagg acctgagcga ccagaaggcc tatgagcagg
     1141 cactgaagca gaacgacatc acggaagacg agctccgcga ggacatccgc gaaaaccttc
     1201 ctgtccagaa ggtacaggag agggtggcgg gcggcgcgga gccgaccgac gaggagatcc
     1261 agaagtacta cgagcagaac aaagaggccc agttcacgac cccggagcag cgctgcgtaa
     1321 ggcacatcct cttcaacaag gaccagaagc agaaggccga ggaggtaaag cagcaactcg
     1381 agaacggcgg ggacttcgcg gcgctcgcca aagagtactc ccaggaccct ggcagcgcgg
     1441 agaaaggcgg ggacctcggc tgcctcggca agggtgagac cgtccccgag ttcgagaagg
     1501 cagccttcgg ggcggagcag ggtgagatcg tcggccccgt ccagaccgag ttcggttacc
     1561 atcttctgga agtcaccgac ataaagccca tgcaggcccg ttctctcgat gaggtcgagt
     1621 cgcagataag gtcccagctc tccactgagc agcagtcgga agagttcaac aagtggctgg
     1681 aagagcagaa aaagaagcgt gacgtgaagt acctggaggg ctacaagccg ccggatcagg
     1741 gctgaactaa ggggaccccg cggtggcgcc gcgccgcgcg taggggtaaa gttataaagt
     1801 tacaaaagta catacaagcg tctgagagcg gcaagggagg ataactgctg atgacggaga
     1861 tcgtcgcggt ccacgggcgg gagatcctgg attccagggg aaacccgacg gtcgaggtgg
     1921 agttggctac ggtgagcggt ttcgtgggcc gggcctcggt cccgtccgga gcctccaccg
     1981 gacagaacga ggcagtcgag ctgagggatg gtgacaagga tcgctacggc ggcaaaggtg
     2041 tactgaccgc cgccacaaac gtggacaccg agctggcgca ggtcgtcgtc gggctcgacg
     2101 cggccgacca gaggaccctc gacctcgcga tgatcgaggc cgacggcacg gagaacaagg
     2161 gccgcctggg ggcgaacgcg atcctcggcg tctcactggc ggctgcgaag gcggcggcgg
     2221 aagcctccgc gctccctctg taccgctacc ttggaggggc gggcgcttac accctgccag
     2281 tgccttgcgc caatatcatg aacggcggcg cgcacgcggc gaacaacgtg gactttcagg
     2341 agttcatggt cgtgcccgtc gggttcgaca cctactcaga ggcgcttcgc tccgtcgccg
     2401 aggtctacca ggttctcaag aagctgctgg cagagagggg tctctccggc ggtatcgggg
     2461 atgagggggg cttcgcgccc gacctggaga gcaacggcgc ggcgctcggg ctcatctccg
     2521 aggccgtcga gaggagcgga tactcccttg gggaccagat ggccttcgcc ctcgaccctg
     2581 cggcctccga gttctacgag gacggcacct acgacctcgc cggcgagggc aagaagctct
     2641 ccagggagga gatggtcgat tactacgtcg ggctgtgcga cgagtatccc atcctcagca
     2701 tcgaggacgg cctcgccgag gacgattggg aggggtgggc catcattacc gggaagctgg
     2761 gtgacagggt ccagctcgtc ggcgacgacc tcttcgtgac gaaccccaag atactcgcgc
     2821 gcggcatcga cgagggtgtg gggaactcca tcctcataaa ggtcaaccag atcggcaccc
     2881 tcacggagac gttcgagacc atggagcttg cgcacaaatc cggctacacc acgatggtca
     2941 gccaccgcag cggggagacg gaggacgcga ctatatccga cctcgccgtt gccgtaaacg
     3001 ccggacagat caagacaggc gcgccggccc gcggtgagcg cgtcgccaag tacaaccagc
     3061 tactccgcat agaggaggcg ctgggaacct cggctttcta tcccggccgg gctgccttca
     3121 acccgagg
//
LOCUS       DATAIR010000069         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_186480, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000069 DATAIR010000000
VERSION     DATAIR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186480"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VHM69_01805"
     CDS             <1..520
                     /locus_tag="VHM69_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2739153.1"
                     /translation="QRSGIPYAEGLIKNRYVGRTFIQPTDGMRQLGIRLKLNPLPSVI
                     EGKRVVVVDDSIVRGNTSRKLVELLFEAGAAEVHFRVSSPPIKGPCYYGIDMDSPDQL
                     VGANLTVEEIRAQIGATTLAYLSTDAMVAATQQRKDRLCCACFDGDYPTTGTASKFDL
                     ENRADYAATRGG"
     gene            705..1181
                     /locus_tag="VHM69_01810"
     CDS             705..1181
                     /locus_tag="VHM69_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739154.1"
                     /translation="MTTEPLHVRRYEAGDKDAVRRLHDDALNEVGAHLGRGHWDEDLD
                     EIEVVYLESGGEFLVGALDGEVVAMGALRRISPEKAEIKRMRVRPDLQGRGYGQCLLD
                     ALHRRAAELGYSTLHLDTTVQQRVAQLFYIKNGYREAGRGSIGPFECIFYERDAIA"
     gene            1174..2190
                     /gene="purM"
                     /locus_tag="VHM69_01815"
     CDS             1174..2190
                     /gene="purM"
                     /locus_tag="VHM69_01815"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2739155.1"
                     /translation="MPEDSYEAAGVSIERGERTVELMRSAVQRTHGPEVLGGPGGFAG
                     LYALAGYRDPVLASTIDGVGTKVLVAREVGRYDSLGVDIVNHCANDVLATGARPLFFL
                     DYVASGRLEPEAVASIVRGAAESCGRLGVALIGGETAEMPGVYGEGDFEIVGVCVGAC
                     ERGDVVGGENVRPGDAVLGLASSGLHTNGYTLARKVLADAGVSYSDRPDSLLRPVGEV
                     FLEPHWAYVREVEALSGAVEVRGMAHVTGGGLVGNLPRALGGLGARLDAGSWEEPAVF
                     RLIRSLGGVPEGEMRRVFNLGIGFCAVVPPEEAERGLEALRGAGCEAWGIGEVVEDRG
                     VEFV"
     gene            2183..>2448
                     /locus_tag="VHM69_01820"
     CDS             2183..>2448
                     /locus_tag="VHM69_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX2739156.1"
                     /translation="MSERLPEGSRFAVLASGSGTNLQALLDAYPDNVAVVAGDKKEAF
                     AFERARRAGVPVEHVDPSSFVSREDYDRELATRIEVHDVGLVVGA"
BASE COUNT          424 a          752 c          870 g          402 t
ORIGIN      
        1 gcagcggagc ggaataccat atgcagaagg cctcataaag aaccgttacg tcgggaggac
       61 cttcatccag ccgaccgacg ggatgcgcca gctcggcatc aggctcaagc tcaaccctct
      121 gccgagcgtg atcgagggca agagagtagt cgtggtggat gactcaatcg tgcgcggcaa
      181 cacgagccgc aagctggtcg agttgctttt cgaggccggc gccgccgagg tccacttcag
      241 ggtctcttcg cccccgatca aaggtccctg ctactacggc atcgacatgg atagcccgga
      301 tcaactcgtc ggcgcgaacc tgacggtcga agagatacgg gcccagatcg gggccaccac
      361 cctcgcctac ctctccacag acgcgatggt cgcggccacg caacagagga aggaccgcct
      421 ctgctgcgcc tgcttcgacg gcgactaccc gacaacaggc acggcgagca agttcgacct
      481 ggagaacagg gccgactacg ctgccacaag gggcggatga gcccctcgtc gcacgcacga
      541 ccggactcgt ggccccccct tcttggtccg caatggtggc gagcgtagtg cgctatccgg
      601 atatcgcaga ggctctgcgc gcggcgatcc tcgacgagtc gcgaaggtcc ggctagctca
      661 tggagacccg tgaggcatca ctcaagagaa gcgccggtga aacgttgacc accgaaccct
      721 tgcacgttcg acgttacgag gctggggaca aagatgccgt caggcgtctg cacgacgacg
      781 cgttgaacga ggtcggagcg catctgggca gggggcattg ggacgaggac ctggacgaga
      841 tcgaagtcgt ctacctcgaa tccggcggcg agttcctggt cggcgcactg gacggggagg
      901 tagtcgcgat gggcgccttg cgacggatct cgcccgagaa agccgagatc aagcgtatgc
      961 gcgtgaggcc ggatctccag ggacgcggct acgggcagtg cctcctcgac gcccttcacc
     1021 gccgcgccgc cgaactcggt tactcgacgc tgcacctcga caccacagtg cagcagcgtg
     1081 tggcccaact cttctatatc aagaacggct accgcgaggc ggggcgaggc agcatagggc
     1141 ctttcgagtg tattttctac gagagggacg cgattgcctg aggacagcta cgaggccgct
     1201 ggcgtctcca tcgaacgcgg agagcgtacc gtcgaactga tgcgctccgc ggtccagagg
     1261 acccacggcc ccgaagtcct cggcgggccc ggcggcttcg ccggtctgta cgccctcgct
     1321 ggctatcgcg acccggtcct cgcctcgacg atcgacggcg tcggcacgaa agtcctggtc
     1381 gcgagagagg tgggacgcta cgactccctg ggtgtggaca tcgtcaacca ctgcgccaac
     1441 gacgtgctcg ccacaggcgc caggccgctg tttttcctcg attacgtcgc gagcggccgt
     1501 ctcgagcccg aggccgtcgc gagtatagta cgcggcgcag ccgaatcctg cgggcgcctc
     1561 ggggtggcgc tcatcggtgg cgagacggcg gagatgccgg gggtctacgg cgagggagac
     1621 ttcgagatcg tcggcgtctg cgtaggagcc tgcgagcgtg gagatgtcgt aggcggcgaa
     1681 aacgtcagac ccggcgacgc cgtgctcggc ctcgcctcca gcggactgca caccaacggc
     1741 tacacgcttg cccgcaaggt gctcgctgac gccggggtct catactccga ccgacctgac
     1801 agcctgctcc ggcccgtcgg agaggtcttc ctcgaaccgc actgggcgta cgtgcgggag
     1861 gtggaggcac ttagcggcgc ggtagaggta cgggggatgg cgcacgtgac gggaggtggg
     1921 ctcgttggga acctgccgcg cgcgctcggt gggctcggtg cgcggcttga cgccggttcc
     1981 tgggaagaac cggcggtctt ccggctgata aggtcgctcg ggggcgtgcc cgagggcgag
     2041 atgcgcaggg tcttcaacct cggcatcggc ttctgcgcgg tggtaccgcc cgaggaggcg
     2101 gagcgagggc tcgaggcgtt gcgcggggct ggttgcgagg cctgggggat cggggaggtt
     2161 gtcgaggata ggggcgtcga gtttgtctga gcggctgccc gagggttcgc gcttcgccgt
     2221 gcttgcgtcg ggttccggga cgaacctgca ggctttgctc gacgcctatc cggacaacgt
     2281 tgccgtggtc gcgggggaca agaaggaggc tttcgcgttc gagcgggcgc ggcgtgccgg
     2341 cgtgcctgtc gagcacgttg atccttcctc gttcgtcagc cgggaggact acgataggga
     2401 gcttgcgacc aggatcgaag tccacgacgt agggctggtg gtgggggc
//
LOCUS       DATAIR010000070         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_96328, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000070 DATAIR010000000
VERSION     DATAIR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96328"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="rsmD"
                     /locus_tag="VHM69_01825"
     CDS             complement(<1..490)
                     /gene="rsmD"
                     /locus_tag="VHM69_01825"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX2739157.1"
                     /translation="MALQGVRVIAGSARGRRLAPVPKNVRPTSDRVRESLFNSLGQFF
                     EGGSVLDLYAGTGALGIEALSRGSERAVFVERDRRVRAVIHENLRRTGFVERAEVIGD
                     DVEPVLGRLSERGELFNLIFADPPYRIAAAEVGGILGRLRSLLTPGGRVVIESGEAPV
                     AGG"
     gene            complement(472..2610)
                     /gene="recG"
                     /locus_tag="VHM69_01830"
     CDS             complement(472..2610)
                     /gene="recG"
                     /locus_tag="VHM69_01830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX2739158.1"
                     /translation="MAWPTSRLGDAALLPGRSTLTDLRSRPVTELPGVGPRIEAALGD
                     LGIASLADMISHYPSRHEDLSNVKRISELRVGERATVVGTVVSTRPVGKPVRGRSPGF
                     SAQLYDGTGYIPVTVWGRSWLLNQLLPDVRVVVSGEVQSRYGIQIAARSIEVVDDPAG
                     DEGPHAGRFVPIYPVNKGIQARRMRTLIHRALDVAGRILDPLPADLLVRHGLPNLHDS
                     IHEVHFPTERARLKAATWRLIFHELFLIQAGLAARKARLDRTEVGGRHTGDGSLLNPF
                     LKSLSFGLTAAQDRVIAEVLGDMRLEKPMRRLLQGDVGSGKTVVAVAALLTAVEAGGQ
                     GAIMAPTEVLAEQHYLSISAALAELPVNVVLVTGSQGASERRAALDQIAGGEAHVVVG
                     THALIQKGVDFKDLSLVVVDEQHRFGVGQRTVFREKGTTPDTLVMTATPIPRTLSLTL
                     YGDLEVSIIDELPPGRKPVETRIVDPAERLGAYEEVRRELEDGRQAYVICPLVEESEA
                     LEDVRAAEELREELAEEIFPERRVGLLHGRMKAQDKREVMASFRAGEVDVLVATVVVE
                     VGVDVPNASAIVIEGAERFGLSQLHQLRGRVCRGLHPPKCFLVGAPKTDDSERRLQAL
                     VEYQDGFKLSEVDLAIRGEGTLFGSRQSGMPDLKVAKLLRDVDVLVEARREAFSIVAG
                     DPTLKRPDHRPLRREIRELLGANVEWLFRE"
     gene            complement(2591..>2843)
                     /locus_tag="VHM69_01835"
     CDS             complement(2591..>2843)
                     /locus_tag="VHM69_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2739159.1"
                     /translation="KLTGGEVVPFKRTRGHRRQMAAMVEEVRPAVEAGRPLYFGHIAA
                     PELLSELAEQLGVEEKLVAEIGGVVGSHVGLGAYGVAHL"
BASE COUNT          469 a          974 c          909 g          491 t
ORIGIN      
        1 cgccacctgc aaccggagct tcgccgctct cgatcacgac ccttcctccg ggcgtgagca
       61 gagagcgtag ccggcccaag ataccaccga cctccgccgc agcgattcta tatggtgggt
      121 ccgcgaagat caaattgaat agttcaccac gctcggagag ccgcccgagc acgggttcga
      181 cgtcgtcacc gatcacctcg gcccgctcca cgaagcccgt cctccggagg ttctcgtgga
      241 tcacggcccg cacccgcctg tcgcgctcga cgaagactgc ccgctcgctc ccgcggctga
      301 gggcctctat cccgagtgcg cccgtccccg cgtacaggtc gagcacgctg ccgccctcga
      361 agaactgccc taatgagttg aacaggctct cccgcacgcg gtcagaggta ggacggacgt
      421 tcttcgggac gggggctagc ctcctaccgc gggcagaccc ggcgatgacc ctcactccct
      481 gaagagccac tcgacgttcg cgccaaggag ctcccggatc tcacgccgca ggggcctgtg
      541 gtcgggacgc tttagcgtgg gatccccggc cacgatcgag aaagcctcgc gcctggcctc
      601 gaccaggacg tcaacgtcgc gcaggagctt ggctactttg aggtcgggca tgccgctctg
      661 gcggctcccg aagagggtgc cctcgccacg gatcgcgagg tcgacctcgg agagcttgaa
      721 gccgtcctgg tactccacca gcgcctggag gcggcgctcg gagtcatcgg tcttcggggc
      781 gccgacgagg aaacacttcg ggggatgcag ccccctgcaa acgcggcccc tgagctggtg
      841 cagctgagac agaccaaacc gctcggcgcc ttcgatcacg atcgcgctcg cgttgggcac
      901 gtcgacaccg acctcgacga ccacggtagc gacgagcacg tcgacctccc cggcgcggaa
      961 agatgccatg acctcgcgtt tgtcctgcgc cttcatcctg ccgtgcagga gcccgacccg
     1021 tctctcgggg aagatctcct cggccagctc ctcccgcagc tcttcggcgg cccgtacgtc
     1081 ttccagtgcc tcggactcct cgacgagagg gcagatcacg tacgcctgtc tcccgtcctc
     1141 cagctcccgc ctgacctctt cgtatgcccc tagccgttcc gccgggtcca cgatcctcgt
     1201 ctcgaccggc tttcgtcccg gcggcagctc gtcgattata gagacttcga ggtcgccgta
     1261 gagcgtcagc gagagcgttc tcggtatggg ggttgccgtc atgacgagcg tgtcgggggt
     1321 cgtccctttc tcacggaaca ccgtccgctg gccgaccccg aagcggtgtt gctcgtccac
     1381 aacgacgaga gagaggtctt tgaaatcgac gcccttctgt atgagggcgt gagtacctac
     1441 gacgacgtgg gcctcgccgc ccgctatctg atcgagggcg gctcgcctct ccgaagctcc
     1501 ctgcgagcct gtgacgagca cgacgttgac cggcaattcg gccaaagccg ctgagatcga
     1561 gaggtaatgt tgctcggcga ggacctcggt gggggccatg atcgcaccct gcccccctgc
     1621 ctcgaccgcg gtgaggagcg cggcgacggc gaccacggtc ttcccgctcc ctacgtcacc
     1681 ttgcaggaga cggcgcatag gtttctccag gcgcatgtcg ccgagtactt cggctattac
     1741 cctatcctgt gcggcggtca ggccgaagga gaggctcttc aggaaagggt tgaggaggct
     1801 tccgtccccg gtgtgcctgc cacctacctc tgtcctatcc agccgcgcct tgcgggccgc
     1861 gagcccggcc tgaatcagga agagctcgtg gaagatcaga cgccacgtcg cggccttgag
     1921 tcgcgcccgc tccgtcggga aatgaacttc gtgtatggag tcgtgcagat tgggcaggcc
     1981 gtgtcgcacg agcaggtcgg cgggcagcgg gtccagaatg cgtccagcca catcgagggc
     2041 gcggtggatg agggtccgca tcctgcgcgc ctgtatgccc ttgttgacgg ggtagatggg
     2101 gacgaaacgt ccggcgtgtg gtccttcatc gcccgcgggg tcgtcgacga cctcgatgct
     2161 cctggcggct atctgaatcc cgtagcggct ctgaacctcg ccagatacga ccaccctcac
     2221 atcgggcagg agctggttga ggagccagct tcgtccccag acggtcacgg ggatatagcc
     2281 cgtgccgtcg tagagttggg ccgagaaccc tggagagcgg cccctgacag gctttccgac
     2341 gggacgggtc gagacaaccg ttcccacgac ggtggccctc tccccaacgc gcagttcgga
     2401 gatcctctta acgttcgaaa ggtcctcgtg gcgcgagggg tagtgtgaga tcatgtcggc
     2461 gagcgaggct atcccgaggt cacccagcgc ggcctcgatg cgcggcccga caccagggag
     2521 ctcggtcacg ggccgggaac gaaggtcggt gagggtcgat cgcccaggga ggagcgcggc
     2581 gtcgcccagt ctagaggtgg gccacgccat aggctcccag gccgacgtga gatcccacga
     2641 ctcccccgat ctcggcgacg agcttctcct cgacacctag ctgctccgcg agttcggaga
     2701 ggagctcggg ggcggcgatg tgcccgaagt agaggggcct acccgcctcc accgccggcc
     2761 tgacctcctc gaccatggca gccatctggc gcctgtgtcc gcgggtacgc ttgaatggca
     2821 cgacctcacc gcccgtcagc ttc
//
LOCUS       DATAIR010000071         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_140415, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000071 DATAIR010000000
VERSION     DATAIR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140415"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VHM69_01840"
     CDS             <1..209
                     /locus_tag="VHM69_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739160.1"
                     /translation="GAASFVSEDRSAATGVALGSIAAIAAAIAGEWLRALAGRKTGLP
                     DYLVALAEDAVVILVGFRSLREVR"
     gene            219..1022
                     /locus_tag="VHM69_01845"
     CDS             219..1022
                     /locus_tag="VHM69_01845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2739161.1"
                     /translation="MADERNRLRKVFDEAALLYDEARPGYPDELFDDVVSLSGIPPEG
                     RILEIGCGTGQATVPFARRGYHVLCIELGENMAALARRNLRMYPQVGVRTGDFEKVPL
                     QESAFDLAVSATAFHWLDKSIAYPKVARSLVPGGAISLFWNEHVHSDASDGFFEAAQR
                     IYEREAPEIVGPEDYKGLPRPDEVPDRTGEIQNTGLFGEVIIRSYLWDVPYDAKSYLR
                     VLNTYSGHRSLRDDSRERLFRGIGDLIDSEYGGSIVKGYLTTLYVAPRK"
     gene            complement(1033..1893)
                     /locus_tag="VHM69_01850"
     CDS             complement(1033..1893)
                     /locus_tag="VHM69_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX2739162.1"
                     /translation="MAEKIVAEGVEGTLGERLRSARPMGGGCIGEVYKVELENGTPLA
                     AKVDRDGESHLEREAYMLSYLREKSDLPVPEVYHGSETLLLMEFVEGSSRFSEGAERH
                     AAELLASLHGITAGAYGHERDTLIGSLDQPNPPTDDWVEFFREQRLMYLARVAYDGGR
                     LPVEDLRRVERLAERVEDLIGEPNPPALIHGDVWSANVLAKGDRITAFLDPALYYADP
                     EIELAFISLFNSFGDPFLERYAEIRGIDGRFFETRRDLYNLYPLLVHVYFFGGGYLDS
                     VRNTLDRFDV"
     gene            complement(1886..>2124)
                     /locus_tag="VHM69_01855"
     CDS             complement(1886..>2124)
                     /locus_tag="VHM69_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEX2739163.1"
                     /translation="CHRFDYILTMDEENFRTVSGLCNGGAEVRPFLDYAPGPETEVPD
                     PYYGGSEGFQHALDLIEGASEGLLQEIRERHLGG"
BASE COUNT          406 a          670 c          655 g          393 t
ORIGIN      
        1 ttggggccgc ctcgttcgtc tcggaagatc ggagcgccgc tacgggcgtc gccctcggct
       61 ccattgcggc tatcgcggcg gccatcgcag gcgaatggct gcgcgcgctc gcggggcgga
      121 agacggggct ccccgattac ctcgtcgcgc tcgccgaaga cgccgtcgtg attctcgtcg
      181 gattccgctc gttgagggaa gtacgttaac cctggctcat ggctgacgaa aggaaccggc
      241 taaggaaggt cttcgatgag gcggcgctgc tctacgatga ggcgcgtccc ggctatcccg
      301 acgagctctt cgacgacgtg gtgtcgctct caggaatacc gccagaaggc agaattctgg
      361 agatcggatg cggcacgggc caggctaccg tgccgttcgc ccggcgcggc taccacgtcc
      421 tctgcatcga actcggcgaa aatatggccg ccctcgcccg acgcaacctc agaatgtatc
      481 cgcaagtggg agtgcgtacc ggagacttcg aaaaagtgcc cttgcaagag agcgcctttg
      541 atctggcggt ctcggccacg gcctttcact ggctggacaa gtcgatagcc tatccgaagg
      601 tcgccaggtc actcgtgccg ggaggcgcta tctccctctt ctggaacgaa cacgtgcaca
      661 gcgacgcgag cgatggcttc ttcgaggccg cacagagaat ctacgagcgt gaggcgccgg
      721 agatcgtcgg tcctgaagat tacaaggggc ttcctcggcc agacgaagtt cctgacagga
      781 cgggtgagat ccagaacacg ggtctgttcg gagaggtgat catccgtagt tacctgtggg
      841 acgtacctta cgacgccaaa agctacctgc gagtcctgaa cacatactca ggccacagga
      901 gcctgcgcga cgacagccgt gaacgcctct ttcgcggtat tggcgacctc atcgactcgg
      961 agtacggtgg cagcatcgtc aagggctacc tgaccaccct gtacgtcgcc ccaaggaagt
     1021 aagcggggaa gttcagacgt cgaaacggtc caaagtattc ctcaccgaat ccaggtaacc
     1081 gccaccgaag aagtagacgt ggaccagtag tggatacagg ttgtacaggt cgcggcgcgt
     1141 ctcgaagaaa cgtccgtcta tgccccggat ctccgcgtag cgttccagaa aggggtcgcc
     1201 gaaagagttg aagaggctga tgaaagccag ttcgatctcg ggatcggcgt agtaaagggc
     1261 aggatccagg aacgcagtga tgcggtctcc cttcgccagc acgttggcac tccagacgtc
     1321 gccgtggatg agggccggag gattgggctc cccgatcaag tcttccaccc gctccgccag
     1381 acgctccacc ctgcgcaggt cctccacggg caacctaccg ccgtcgtagg cgacccgcgc
     1441 cagatacatt agtctttgct cccggaaaaa ctccacccaa tcgtccgtcg gcggattcgg
     1501 ctggtcgagg ctgccgatga gcgtgtccct ctcgtggccg taggcgcctg cggtgattcc
     1561 gtgcagagaa gcgagcaact ccgcggcgtg ccgttcggca ccttccgaga agcggctgct
     1621 cccctcgacg aactccataa ggagcagcgt ctctgagccg tggtagacct cgggaacggg
     1681 gaggtcgctc ttctcgcgca ggtagctgag catgtacgcc tcgcgctcca ggtgcgactc
     1741 gccgtcccta tcgaccttgg cggcgagcgg cgtgccgttc tcgagctcga ctttgtagac
     1801 ctcgccgatg caacctccgc ccatcgggcg ggcgctacga agtctttcgc ccagagtacc
     1861 ctccactccc tcggccacga tcttctcagc cacccaggtg ccgctccctg atctcctgca
     1921 gcagaccttc ggacgctccc tctatgaggt caagcgcgtg ctggaagccc tcggagccgc
     1981 cgtagtaggg gtcggggacc tcggtctcgg gaccgggcgc gtagtcgagg aaaggacgga
     2041 cttctgcccc gccattgcag agccccgaaa ctgtccgaaa gttctcctcg tccatcgtca
     2101 gtatgtagtc gaacctgtgg cagt
//
LOCUS       DATAIR010000072         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_324986, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000072 DATAIR010000000
VERSION     DATAIR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324986"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHM69_01860"
     CDS             complement(<1..227)
                     /locus_tag="VHM69_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739164.1"
                     /translation="MRETAAIDRLTEVVEGVLPEGTELVEARFSGGPLLTLLLDREDG
                     PVDHAFCSRVASVVAPAVEAEGYDGPIEVSSP"
     gene            604..2142
                     /locus_tag="VHM69_01865"
     CDS             604..2142
                     /locus_tag="VHM69_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX2739165.1"
                     /translation="MGSRVTRREFLTAVGATYIALTTTVGCELLDRDSKVRDVNSPKA
                     DPLRTPKVRPLPGVSDAPSQGVWVFRSRPDLSPPSVEVKTQSNNTAPGYIFVAPEKGG
                     SGQGGSMILDDSGQVVWFRPLRTNGRAMNFKVQTYQGNPVLTWGETPGEYVIFDDSYR
                     EIARITAANGFNGDHHEFSITPQDTALITIYDAVPWDLTPVGGQKDSLAWQGVIQELD
                     LETGEVIFEWHSLDHVDLEETYVTPEEDHYAGIDYFHINSLDVDHDDNLLVSARETSA
                     VYKIDRSTGEVIWRLGGKKSDFEMGPGTRFAFQHDARRQPDGTITIFDNGDTVFVNGD
                     PKAVEESRGIVLEVDEEQMTASLVREYNHPGEQYADAAGNMQVLPNSNVFIGWGRALA
                     ISEFTKDGELIFDASLSPQNRSYRAFRLPWRSHPSTRPAAVAERSSEEEISVYASWNG
                     ATEVATWEVLAGTGPEQLDSVGSVARNGFETAMVVQTSEPYVAMRAKDRSGRVLGTSE
                     PVNV"
     gene            complement(2258..3331)
                     /gene="ispG"
                     /locus_tag="VHM69_01870"
                     /gene_synonym="gcpE"
     CDS             complement(2258..3331)
                     /gene="ispG"
                     /locus_tag="VHM69_01870"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2739166.1"
                     /translation="MTEAALRERPVTSRRIIVGDVPIGGGAQVAVQSMTNTMTYDVEA
                     TVQQVYDLNAAGADLVRVSVNGSKALKGFREVVWRSPVPLIADIHFDYRMALGAADAG
                     AACVRINPGNVGSDDRYRLVIEKCQETGTAMRIGVNSGSVEKRFWDLPKAEALVASAL
                     HKVEIAEEMGFFDFKVSLKASHVPEMVEANKKFREHSDAPLHLGVTEAGTKLPGAIKS
                     AAALGQLLPYGIGDTIRISLAEDPVEEIPVAYHILSALDLRHRGPNVIACPSCARTLG
                     FDVIGMAARVEEGLKHFEDHFTVAVMGCIVNGPGEARDADYGVAGGKDDGVIFSKGQP
                     LRKVGRDEIYDSLFEEIAKDGRK"
     gene            complement(3365..>4263)
                     /gene="rseP"
                     /locus_tag="VHM69_01875"
     CDS             complement(3365..>4263)
                     /gene="rseP"
                     /locus_tag="VHM69_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX2739167.1"
                     /translation="KTVYSFRIILLGGFAKMAGMGDGAEGPDTYHAKAAWRRALIILA
                     GPAANIVAAVVILAGVYMFQGVVTDVKPVVSAVEPGSMAKEVGIQAGDRIVGLDNDRV
                     KTWDGFERKMAGREPGERVSVTVERDGTEKTFSGNLGADPEDKDRALVGVRPELVTTT
                     YGPLESVGLATQRCVEITVKLGTFVGKLVTGEKDLYDNVTGPVGIASVGSTAVNQGFF
                     LPLLALISLNLAIFNLLPILPLDGGHLFFIAAEKVIGRPVSEQTMGKIAAFGIVLVLA
                     LFLFATYADLSKIFTGQPFIPD"
BASE COUNT          855 a         1368 c         1245 g          795 t
ORIGIN      
        1 ggcgacgaga cctcgatcgg accatcgtag ccctccgcct cgactgccgg cgcgacgacg
       61 gaggcaacac gcgagcagaa cgcgtgatcg accgggccgt cctcccggtc gaggagcaga
      121 gtgagcagag gcccccctga gaaccgggcc tctaccagct cggttccctc ggggagtacg
      181 ccctcaacta cctctgtgag cctgtcaatt gccgccgtct ctctcacctt ccctgaacga
      241 taaaaaaagc aacgggcccg aagacccctt gctcccttac aacacgcacg attatatctc
      301 agagtatgta ggttacaagg cagcggagcc ccctctaacc gcctgccttc taccacgaac
      361 acgcacagaa ggcgtgagca gatcaagccg tccttgaggc ggactatgca ggaccgcgcg
      421 ggcctcgtag taagaggcag tctcgaaaat cggctgccag gacccctgaa ttcatcggag
      481 ttcgctcttc ccaccggccc agcgttcatg cataattcat cgcccagtga ttccatggat
      541 gatttcggca gagagggaag tccaggttgt caagatgcac acttgaaagt agagggtccg
      601 gctatgggca gtagggtcac ccgacgcgag tttctcacag cggtgggcgc cacgtacatc
      661 gcgttgacaa ccacggtagg ttgcgaactg ctcgatcgcg actcaaaggt cagagacgtc
      721 aactccccaa aggctgatcc cttgcgcacc ccgaaggtcc ggcccttgcc gggagtttca
      781 gacgcgccat cccagggtgt gtgggtcttc cgctcgcgtc ctgaccttag cccgccctcc
      841 gtcgaggtga agacgcaatc gaacaacacg gcccccggct atatcttcgt cgctcccgag
      901 aagggaggct ctgggcaggg tggatctatg atccttgacg atagcgggca ggtggtgtgg
      961 ttccgtcccc ttcgcacgaa cgggcgcgcg atgaacttca aagtgcagac ctatcaggga
     1021 aatcccgtgc ttacgtgggg ggagacacct ggcgaatacg tgatcttcga cgattcctac
     1081 cgcgagatag cccgcatcac ggctgccaac ggctttaacg gagaccacca cgagttctcc
     1141 atcacgcctc aagacaccgc actgatcacg atctacgacg ctgtgccctg ggaccttact
     1201 ccggtcggtg gccagaaaga cagcctggcc tggcaaggcg tcatccagga actcgacctc
     1261 gagacgggag aggttatctt cgaatggcac agcctggatc acgttgacct tgaggagacc
     1321 tacgttactc cggaagagga tcactacgcc ggcatcgact acttccacat caactccttg
     1381 gacgttgacc acgacgacaa cctgctcgtc tctgcccgcg aaacctccgc cgtctacaag
     1441 attgaccgca gcacgggcga ggtcatctgg cgcctcggtg ggaagaagag tgacttcgag
     1501 atgggccccg gtaccagatt tgccttccag cacgacgcca ggcgccagcc cgacggcaca
     1561 atcaccatct tcgacaatgg cgacacggtc tttgtgaacg gcgacccgaa ggcggtagaa
     1621 gagtcccgcg gcatcgtact ggaggtcgac gaagagcaga tgacggcctc gctggtacgc
     1681 gagtacaacc accctggcga gcaatacgcc gacgcggcgg gaaatatgca ggtgcttccg
     1741 aactccaacg tgttcatagg ttggggtagg gcactggcaa tctctgagtt caccaaggat
     1801 ggggagttga tcttcgacgc cagcctgtcg ccccaaaaca ggtcctacag ggccttccgc
     1861 ctcccgtgga gatctcaccc gagcacgcgg cccgccgcgg tggcagagcg ctcctccgaa
     1921 gaagagatca gtgtctacgc cagctggaac ggagccactg aggttgccac ctgggaggtg
     1981 ctcgccggca cgggcccaga gcaactagac tccgtgggat cggtagccag gaacggcttc
     2041 gagacggcca tggtggtgca aacctccgag ccgtacgtcg ccatgcgagc caaagaccgt
     2101 tctggtcggg tgctcggcac ctccgaacct gtcaacgtgt gagatagagc atcaagactt
     2161 ttcggacagt ttcaaggcga agttcgcaga gcgcttcttt cgtgaagtcc ggagaggcct
     2221 gcagcttcgt tttgcggttc ttcctcgaga agccccgcta cttccgcccg tccttagcta
     2281 tctcctcgaa gagcgagtcg tagatctcat cccggcccac cttccgcaaa ggctggccct
     2341 tggagaagat cacaccgtcg tccttgccgc cagcgacacc gtagtcggcg tcgcgtgcct
     2401 cgcctggacc gttgacgatg catcccatga cagcgacggt gaagtgatcc tcgaagtgct
     2461 tgagcccctc ttcgaccctg gctgccatcc ctataacgtc gaagccgagg gtacgcgcgc
     2521 agctgggaca ggcgatgacg ttcggccccc tgtgcctcag gtcgagggcg gaaaggatgt
     2581 ggtacgcaac gggtatctcc tcgacggggt cctccgcgag cgagatgcgg atggtgtccc
     2641 cgatcccgta aggcaggagt tgacccagcg ccgccgcgct ctttatggcg cccggaagct
     2701 tggtgcccgc ctcggtaacg ccgaggtgca gcggcgcgtc ggagtgctcg cggaacttct
     2761 tgttcgcctc gaccatctcg ggtacgtggc tggctttcaa cgacaccttg aagtcgaaga
     2821 agcccatctc ctcggcgatc tccaccttgt gaagcgcgct cgcaacgagc gcctcggcct
     2881 tcggaagatc ccagaatcgc ttctcgacgg accccgagtt gaccccgatg cgcatggccg
     2941 tgcccgtctc ctgacacttc tctataacga gacggtagcg gtcgtcgctc ccgacgttgc
     3001 cagggttgat gcggacgcac gcggcgcccg cgtcggcggc cccgagggcc atcctgtagt
     3061 cgaagtggat gtccgctata agcggcacgg gcgagcgcca gacgacttcc ctgaaaccct
     3121 tcagcgcctt ggagccgttc acggagacgc gcacgaggtc ggccccggcg gcgttcaggt
     3181 cgtagacctg ctgcaccgtg gcctccacgt cgtaggtcat cgtgttggtc atcgactgca
     3241 cggccacctg ggcgccgccg cctatcggca cgtcgccgac tattatccgc cggctcgtga
     3301 ccgggcgttc ccgcagtgct gcctctgtca tctcactccc atttacgtct cggacatccc
     3361 tatcttaatc cgggatgaac ggctgtccag tgaagatctt actaaggtcc gcgtaggtcg
     3421 cgaaaaggaa cagggcgagg acgaggacga tcccgaaggc cgcgatcttg cccatcgtct
     3481 gctcgcttac gggcctgcca ataactttct ccgccgcaat gaagaaaagg tgcccgccgt
     3541 cgagcggcag gatcggcaac aggttgaaga tcgcgaggtt gaggctgatg agggcgagaa
     3601 gcggaaggaa gaagccctgg tttacagcgg tgctcccgac cgaggcgatc ccgacaggtc
     3661 ccgtcacgtt gtcgtagaga tccttctccc cagttacgag cttcccgaca aacgtaccga
     3721 gcttgacggt tatctcgacg cacctctgcg tggcgagacc gaccgactcc aacggcccat
     3781 acgtcgtcgt aactaactca ggtcgtacac caacgagcgc ccgatccttg tcttcgggat
     3841 cagcgcccaa atttccactg aaggtcttct cagtaccgtc gcgctcgacg gttacgctca
     3901 cccgctctcc aggctcgcgg ccagccatct tccgctcgaa accgtcccag gtcttaacac
     3961 ggtcgttatc aagaccgacg attctgtcgc cggcctggat cccgacctct ttcgccatcg
     4021 agcccggctc gacggcgctt acgacgggct ttacgtccgt gactacgccc tggaacatgt
     4081 agacaccggc gagaatgacg acggcggcca caatattggc ggcgggaccg gcgaggatga
     4141 ttaaagccct ccgccaggca gctttggcgt gataggtgtc gggcccctcc gcgccgtccc
     4201 ccataccggc catcttggca aacccaccta gtagtattat gcggaaagag tagacggtct
     4261 tcc
//
LOCUS       DATAIR010000073         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_419467, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000073 DATAIR010000000
VERSION     DATAIR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419467"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1790)
                     /locus_tag="VHM69_01880"
     CDS             complement(246..1790)
                     /locus_tag="VHM69_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX2739168.1"
                     /translation="MSFDPRVGADPFRVWLDAAHAWSDGMLGANKVLAKAARRSSAET
                     QAVLNAASAARGTPDIATITRLVDLSRAQWFLWTGAGLAAGERVGRSVPAGILPPVTY
                     SPRALVLGTVAADLYLGYAALRERTRWFPGLVSDEDWETQHRRGAARVLDAAEALGGV
                     LIKAGQFASTRPDLLPSAYITTLSSLQDRVPPQPYATIREAVAGELRRPPEEVFSSFE
                     KEPVAAASIAQVHRASLRDGRDVAVKVQYPGISGLIEADLDALEAIFDAVARLEPGIR
                     LRPISDYLRWTLPLELDFVREAGAIADLRRVLAERDDVVVPEVIGELTSGRLLVMEYV
                     EGVKITDVETMKKSGIDPSEVAWLLNDVYAEQMFTHGVLHADPHPGNLLVEPGRPRLV
                     LLDHGLTLGLEPSFIGALDRLVSALGSGDLEGVTASLGEAGLPIDEDTDLDTLLKLVG
                     VLLGGGQQEADLGGFGLKLGASVGDIPPKMLLVGRAIGLLDGITRQLDPSLDALEIVG
                     RHTRAS"
     gene            complement(1881..>3270)
                     /locus_tag="VHM69_01885"
     CDS             complement(1881..>3270)
                     /locus_tag="VHM69_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX2739169.1"
                     /translation="VFVVGAYLLLNLVVVAVGLYECVVHPAIVTDWQSKLFSNYGNPL
                     AILGVSLLAFPKLALGLSGFETGVSMMPLVRGDEGDEPQRPRGRIRNTHRMLTVAAII
                     MSCYLITTSFVTTLLIPAKEFQAGGSANGRALAYVAHEHLGEVFGTIYDLSTIAILWF
                     AGASAMAGLLNIVPRYLPRYGMAPEWGRAIRPLVLVYITISVVITIIFQASVDEQAGA
                     YATGVLAMMTSAAFAVSLSAWRRGSRRAAFAFGAIAFVFVYAFVANEIQRPDGILIAA
                     FFIVTIILTSLVSRVWRTTELRTEKIEVDETAQRFIDEASEGENIHIIANRRQTGDAR
                     DYLLKEKEQREYTRIPSDVILFLEVDVEDPSEFKDVLEVRGVDVNGYRVLRAESTTVP
                     NAIAAFLLHLRDTTGKETHCYFDWTQGNPLVYLFRYILLGEGDTAPVTHEVLRGAERD
                     PERRPVIHVAGK"
BASE COUNT          601 a         1035 c         1038 g          596 t
ORIGIN      
        1 tgaaccgaag atccagataa agttctcggg gcttatcccg ccggactcgc catcagttgg
       61 ccgacggtgc ctcttcagat tgtggatact gcgtgagatc ctgcgccgcg tcctccatag
      121 cctgcccttg atgttgcggg cgagatctga agctctgaga ctcgtgattt caggcatcgg
      181 acgaagaacc tttctgatga acctccctaa ggttagggaa accgtcccct cctgtaaagc
      241 gtggtctagg aggcgcgggt gtggcgaccg actatctcca gcgcgtcgag actcgggtcg
      301 agctggcggg tgatgccgtc gagcagacct atggcgcgcc cgacgagcaa cattttcggc
      361 ggtatgtctc cgacgctcgc accgagcttg aggccgaagc cccccagatc cgcctcctgc
      421 tgcccgccgc cgagcaacac cccgacgagc ttgagcagtg tatcgaggtc agtgtcctcg
      481 tctattggca gccccgcctc gccgagggac gccgtgactc cttccaggtc accacttcct
      541 aacgcggaga cgagccggtc gagcgcgccg ataaaggagg gctcaaggcc aagggtgagg
      601 ccgtggtcga ggaggacgag gcgaggtcgt ccaggctcaa cgaggaggtt gccagggtgc
      661 gggtcggcgt gcagtacgcc gtgggtgaac atctgttccg cgtaaacgtc gttgagcagc
      721 caggcaacct cgctcgggtc tatgcccgac ttcttcatgg tctctacgtc tgtgatcttc
      781 acgccctcga cgtactccat gacgagcagg cgtccgctcg tcaactcccc gatcacctcg
      841 gggacgacca cgtcatcacg ttccgcgagc accctccgca ggtccgcgat cgcccctgcc
      901 tcccttacga agtcaagctc cagaggcagg gtccatctca ggtagtccga gatcggccgc
      961 aagcggatgc caggttccaa tcgcgcgaca gcatcgaaga tagcctccag cgcgtcgagg
     1021 tcggcctcga tgaggccgga gatgcctggg tactggacct tgacggccac atccctgccg
     1081 tcgcgcaaac tcgcgcggtg cacctgggcg atggaggctg cggccacggg ctccttctcg
     1141 aaagaagaga agacttcctc cggcggacgg cgaagttcgc cggccacagc ctccctgatg
     1201 gttgcatacg gttgcggcgg gacacggtcc tggagcgagg agagcgttgt gatgtaggcg
     1261 gagggtagaa gatccggcct cgtcgaggcg aactgtccgg cctttatgag cacccctccg
     1321 agcgcctccg ccgcgtcgag cacgcgcgcc gccccacggc ggtgctgagt ctcccagtcc
     1381 tcatctgaca cgaggccggg gaaccagcgc gtccgctcgc gcagcgccgc gtagccaaga
     1441 tacaggtcag cggcgacggt cccgagcacg agcgcccgag gcgagtacgt gacgggaggt
     1501 aagatacccg cagggacgct gcgccccact cgctctcccg ccgcgagccc tgcccccgtc
     1561 cacaggaacc actgggcccg cgaaaggtcc acgagccggg ttatggtcgc aatgtccggc
     1621 gttcctcgtg ccgcggacgc cgcgttcagt acggcctgcg tctctgcgga ggaccgtctg
     1681 gccgccttcg cgagtacctt gttggccccg agcattccgt cgctccacgc gtgggcggca
     1741 tcgagccaga cgcggaacgg gtccgctccg acgcggggat caaagctcat gtctcgctcc
     1801 ctggcacggc ggttgagtat acgcgaagga cgcgggagat cgacgtgaac gggttcatca
     1861 cgaccggtac ggccagggct ttatttgcct gcgacgtgga tgacggggcg ccgttcggga
     1921 tcccgctccg ctcctctcag cacttcgtgg gtgaccgggg ccgtatcacc ctctcctaac
     1981 aagatgtagc ggaacaggta cacgagtgga ttaccctgcg tccaatcgaa gtagcagtgc
     2041 gtttccttgc ctgtagtgtc gcgcaggtgg agcaggaagg cggcgattgc gttcggaacc
     2101 gtcgtactct ccgcacgaag taccctgtag ccgtttacat ccacgcccct cacctctagt
     2161 acgtccttga attcggatgg gtcttccacg tctacttcca ggaaaagtat tacgtccgat
     2221 gggatgcggg tgtactcgcg ttgctccttc tccttcaaaa gataatcgcg cgcgtcgccc
     2281 gtctggcgac gattggcgat aatgtggata ttctctcctt cgctcgcttc gtcgatgaac
     2341 ctctgggcgg tttcgtctac ctcgatcttc tcggtgcgta gctctgtcgt gcgccacact
     2401 ctggagacca gggaggtgag gattatcgtg acgatgaaga acgcggcgat caagatgccg
     2461 tcgggacgct gtatctcgtt ggccacgaag gcgtagacga agacgaacgc gatggcgccg
     2521 aaagcgaatg ccgcgcgcct ggagccccgg cgccaggccg agagcgagac ggcgaaggcc
     2581 gcggaggtca tcatcgccag gacgcctgtg gcgtaggcgc cggcctgctc gtcaacgctc
     2641 gcctggaaga taatcgtaat gacgacggag atcgtgatgt agaccaggac cagaggccgt
     2701 atcgcccgcc cccattccgg cgccattccg tagcggggaa ggtaacgcgg cacgatgttc
     2761 aagagccccg ccatcgccga ggctcccgcg aaccagagaa tggcgatggt ggagaggtcg
     2821 tagatggtgc caaacacctc gccaaggtgc tcgtgtgcca cgtaggccag ggcgcgaccg
     2881 ttggcgctgc cgcctgcctg gaactccttc gccggtatca ggagggtcgt cacgaagctc
     2941 gtggtgatca ggtagcagct catgatgatc gcggcgacgg tcaacatcct gtgcgtgttg
     3001 cgtattcgtc cccgtggacg ctgtggttcg tccccttcgt cgccccgcac gagcggcatc
     3061 atgctaacgc ccgtctcgaa ccccgagagt ccgagcgcca gcttcgggaa cgccagcagt
     3121 gatacgccca gtatcgcgag gggattgccg tagttggaga acagcttgga ctgccaatca
     3181 gtgacgatgg cgggatgcac aacgcattcg tagagcccca cggcgacgac gacgaggttc
     3241 agcagcaagt aggctcccac cacgaacacc
//
LOCUS       DATAIR010000074         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_281404, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000074 DATAIR010000000
VERSION     DATAIR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281404"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VHM69_01890"
     CDS             complement(<1..275)
                     /locus_tag="VHM69_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX2739170.1"
                     /translation="MTGELFPRDAYLREFEARIVKLEGREIILDPTAFYPGGGGQPAD
                     KGSLGIGPVRTNVVDARREGDEIVHVLDRAIPDTVRDLKGALDWERR"
     gene            complement(272..1030)
                     /locus_tag="VHM69_01895"
     CDS             complement(272..1030)
                     /locus_tag="VHM69_01895"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX2739171.1"
                     /translation="MIDYHLHVIAHGDRPMTVENILEYCEVAKARGIRQMGITEHDRY
                     LDEIDLAAFQEARELSHDVELRLGIEIDFVPGAEERMEHDATALPYDYVIGSVHRVDN
                     EEVDRATDQRIYDRYENYDLYAAYYANVRKAALSGRFEILGHPDLIKIFRHFPERDIT
                     PILEETADAVAESGIVVDVNAAGLRKPIGEVYPARNFLEMFHRRGVPIVLSSDAHAPD
                     EVAAGYDTSLKLVHDVGYREVVTFKNHERGNLSL"
     gene            1122..2159
                     /gene="cax"
                     /locus_tag="VHM69_01900"
     CDS             1122..2159
                     /gene="cax"
                     /locus_tag="VHM69_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2739172.1"
                     /translation="MFGKVAYGATAALTVAVVVAELLHAPILLIFAVSAFALIGLAWV
                     LGQATESLGHHSGPRIGGILNATFGNAAELIITIFALSAGLTTVVKASIIGSIIGNVL
                     LVLGASLLFGGLKNGTQSFSAEIAGINASMLAIVAAALSLPTIFAFTNPGSGPTTLSI
                     EVAVVMFVLYILYLIYILRSPEGGAAGGEGHAPFGRTASLVLLFAATAAVAFASEAFV
                     GAIEPLTEEYGISELFVGVILVPIVGNIAEHIVGVQIAYKNNMDFSMGISLGSSIQVA
                     LLVTPILVFLGPLLGTPLHLTFSPLELGALGASVVVTGFVALDGKSNWLEGAMLCGVY
                     VIAALAFYFSP"
     gene            complement(2144..>2477)
                     /locus_tag="VHM69_01905"
     CDS             complement(2144..>2477)
                     /locus_tag="VHM69_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739173.1"
                     /translation="EGVGDYVAGSGGGLTPAGFVEGVSSFENPEKAHLFRWGLGEVIT
                     ALAGAGLRITTLEEYPYTNGERSFSGMRELTGRRMFPPEGVPALPLMYGIRAEKCLRS
                     LKPPHGEK"
BASE COUNT          399 a          805 c          747 g          526 t
ORIGIN      
        1 aaccgccgct cccagtcaag cgcgcccttc agatcccgca ccgtatcagg gatggctctg
       61 tccaggacgt ggacgatctc gtcaccctcc cgtctcgcat caacgacgtt tgtccttaca
      121 ggacctatgc ccagagaccc cttgtccgcg ggctggcctc cgccgccggg atagaaagcg
      181 gtagggtcga gaataatctc acggccttcc agctttacga tgcgggcctc gaactcacgc
      241 aggtaagcat cccgcgggaa gagttctcca gtcacaggga aagatttccc cgctcgtggt
      301 tcttgaaggt gacgacctcg cggtatccga cgtcgtggac tagctttagg ctcgtgtcgt
      361 agcccgctgc tacctcgtcg ggggcgtgag cgtctgagga gaggacgata gggacgcccc
      421 tgcggtggaa catctccagg aaattcctgg cgggatagac ttcaccgatg ggttttctca
      481 gacctgcagc gttaacgtcg acgacgatgc cggactcggc gacggcgtcc gcggtctcct
      541 caaggatcgg ggtgatgtcc ctctcgggga agtggcggaa gatctttatg aggtcggggt
      601 ggccgaggat ctcgaaccgc cccgagagcg cggccttgcg gacatttgca tagtacgccg
      661 cgtagaggtc gtagttctcg tacctgtcgt agatcctctg gtccgtggcc ctgtccacct
      721 cctcgttatc caccctgtgg acgctgccga tgacgtagtc gtaggggagg gcagtggcgt
      781 cgtgttccat ccgctcctcg gcgccaggta cgaagtcgat ctcgatcccc agccgaagct
      841 ccacgtcgtg cgaaagttcg cgggcctcct ggaatgcggc gaggtctatc tcgtccaggt
      901 agcggtcgtg ctcggtgatg cccatctggc ggatgcccct cgctttggcg acctcgcagt
      961 actccagaat gttctccacg gtcatcggac ggtcgccgtg ggctatgacg tgcaggtggt
     1021 agtcgatcaa aggtttgtct ccagaggtcg tgacgcttga cccttccatc ttataagata
     1081 ttcgtcatgt ttatgcgtag cgagcctcgg agggagccgc tttgttcggc aaagtagcct
     1141 atggtgcaac ggccgcgctc acggtggcgg tcgttgtcgc ggagcttctg cacgccccta
     1201 ttctcctcat cttcgccgtc tctgccttcg ccctgatcgg gctggcgtgg gtgctcgggc
     1261 aggctaccga gagcctcggt catcactcgg ggccgcgcat cggcggcata ctgaacgcca
     1321 ccttcggcaa cgcggcggag ctcataatca cgatcttcgc cctttcggcg ggtctcacca
     1381 cggtggtgaa ggcatcgatc atcggctcga tcatcggcaa cgtcctgctc gtgctcgggg
     1441 cgagcctcct cttcgggggc ctgaagaacg gcacacagag cttctccgcg gagatcgcgg
     1501 gcatcaacgc ctcgatgctc gccatcgttg cagcggcgct ctccctgccg accatattcg
     1561 cgttcacgaa cccgggatca ggaccgacca ccctgagcat cgaggtggcc gtggtgatgt
     1621 tcgtgctcta catcctgtac ctgatctaca tcctccgcag cccggagggc ggggccgcgg
     1681 gaggcgaagg acacgccccg ttcggcagga ctgcgtcgct ggtgctcctg ttcgccgcga
     1741 ctgccgcggt ggccttcgct tcggaggctt tcgtcggggc catagagccc ctgacggagg
     1801 agtacgggat ctccgagctc ttcgttggag tgatcctcgt accgatagtc ggcaacatcg
     1861 cagagcacat cgtcggcgtc cagatcgcct acaagaacaa catggacttc tcgatgggca
     1921 tctctcttgg ctcctcgatc caggtcgccc tgctcgtcac ccccatcctg gtcttcctcg
     1981 gtcccctgct cggcaccccc ctgcacctga ccttcagccc gctggaactc ggtgccctcg
     2041 gggcctccgt tgtggtaacg ggcttcgtcg ccctggacgg caagtccaac tggctagaag
     2101 gcgcgatgct ctgcggcgtc tacgtcatcg ccgctctcgc cttctacttt tcgccatgag
     2161 gaggtttcag gctacgcagg catttctccg ccctgatgcc gtacatcagg ggcaaggcag
     2221 gcacgccttc gggcgggaac atacgtctgc ccgttagctc gcgcatcccg gagaagctcc
     2281 gttccccgtt cgtgtaaggg tactcttcga gggttgtgat cctcaatccc gccccggcca
     2341 gcgcagtgat gacctccccg aggccccagc ggaagaggtg ggccttttcg gggttctcga
     2401 aactacttac cccctcgacg aacccggccg gtgtgagacc tccgcctgag ccggccacgt
     2461 agtctccgac cccctct
//
LOCUS       DATAIR010000075         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_186631, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000075 DATAIR010000000
VERSION     DATAIR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186631"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..677
                     /locus_tag="VHM69_01910"
     CDS             57..677
                     /locus_tag="VHM69_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2739174.1"
                     /translation="MIGGLLAELVRWVFDVVHSFGYVGIFVLILAGSLFLPVPTELTL
                     PLLGFLVGHGRLSFVPVLLTATVARVSAAMIFYILGLRIREARLRRLIKRSRLIFVSD
                     LEAASSVFERHGGKAILVGHLIPGVGALVSVPAGLKRMPLRWRFLAYTILGCTLWNGT
                     LIGLGWALGSRWRIVEVFASFVGLGFLTIVVVAALWFIRRRRRTNR"
     gene            complement(730..1038)
                     /locus_tag="VHM69_01915"
     CDS             complement(730..1038)
                     /locus_tag="VHM69_01915"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX2739175.1"
                     /translation="MAIEPLLAIVLMALATYATRAGGLWVASRFELSERAGAWLDQIP
                     GAILISLVAPAVLTGGLAEALAAVAVVIVALRTGSLPAAMVTGVGTVLVLRAMETSLL
                     "
     gene            complement(1028..1708)
                     /locus_tag="VHM69_01920"
     CDS             complement(1028..1708)
                     /locus_tag="VHM69_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX2739176.1"
                     /translation="MTFTLSGALAGARRCVPIAISGFAIGLVFGTLAGQAGLGAGEAA
                     LMSAMVFSGAAQFVVIGLWSAPLPVATIVLATLVVGLRHLLMGAALGPVFSKLPRLKA
                     YGSVFFMADENWALTMGEFAKGRRDAAFLIGGGLLMFAAWTGSTLVGGVFGGAVENPS
                     RWGLDFAFTAVFLALIAGMWKGRSNILPWTVAAMVAVAAHHWLPGQWYILLGGLAGSI
                     AGVVRRGN"
     gene            complement(1765..>2369)
                     /locus_tag="VHM69_01925"
     CDS             complement(1765..>2369)
                     /locus_tag="VHM69_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739177.1"
                     /translation="QAVGEPFDLELARERREELLREAEERRIARALRGTRRNAGEDRR
                     GGVEVRCGVVRDEAAVAELLELNGMPRWIAFEERFVVARKDGEVLGALRYRTESKRLL
                     LGLLVVDPWAGEEPIARALYRGAGELAEEMGVGEVVVSATRSRYPGGAGYRRWGRSWR
                     LDVGETLDHRGQISGSGWHRMSILLAELVSSFYRPFQRAR"
BASE COUNT          441 a          826 c          700 g          402 t
ORIGIN      
        1 ctgcatcgca tggtgcataa ttaggctgtc tcgcggagga gataggagga taagggttga
       61 taggtggtct gctcgccgaa ctcgtacggt gggtatttga cgtagtccac tctttcgggt
      121 acgtcggcat attcgtactc atccttgcgg gtagcctgtt cctgcctgta ccgacggagc
      181 tgacgctccc gctcctcggc ttcctggtcg ggcacgggcg cctctcgttc gtacctgtcc
      241 tgctgactgc tacggtggcg agggtgagcg ccgccatgat cttctatatc ctcgggctga
      301 ggatcaggga ggcgaggttg cggcgtttga tcaagcgctc caggctcatt ttcgtatctg
      361 acctggaggc ggcgagtagc gtgttcgagc gccacggggg gaaggctatc ctggtcgggc
      421 atctcatccc cggcgtgggt gccctggtct cggtgccggc cggcctcaag cgtatgcctc
      481 tgcgctggcg cttccttgca tacaccatcc tcggatgtac cttgtggaac ggcactctga
      541 tcggcctggg ttgggccctc ggcagccgat ggaggatagt tgaggtgttc gcgtctttcg
      601 tcgggcttgg gttcctgaca atcgtcgtcg tagcggcact gtggttcatc cggcgccgtc
      661 ggagaacaaa caggtgaacg acgccccgaa ggcggcggga gattattgtg cgggccgatc
      721 acggcaaagt cacaataggc tcgtctccat ggcccgaagc accagaacgg tccctacccc
      781 ggtcaccata gcggcgggca ggctgccggt cctcagggcg acgatcacga cggccacggc
      841 ggccagtgcc tccgcgagcc cgcccgtcag gacggcgggg gcgacgagcg agatcaggat
      901 ggcgcccggt atctgatcca accacgcacc ggcacgctcc gaaagctcga accggctcgc
      961 cacccagagc cctccggccc gggttgcgta ggtcgcgagt gccatcagaa ctatcgcgag
     1021 caggggctca attgccacgc cgcaccaccc ctgcgatgct tccggccagc ccgccgagca
     1081 gtatgtacca ctgtccgggc agccagtggt gcgccgccac ggccaccatt gccgcgaccg
     1141 tccaggggag gatgtttgat ctccccttcc acatcccagc gatgagagcg aggaagacgg
     1201 cggtgaacgc gaagtcgagt ccccagcgcg aagggttttc gaccgcgccg ccaaaaacgc
     1261 ctccgaccag cgtagaccct gtccaggcgg cgaacatcaa tagcccaccg ccgatcagga
     1321 acgcggcgtc gcggcgtcct ttggcgaact cgcccatggt gagcgcccag ttctcgtccg
     1381 ccatgaagaa cacggaccca taagccttga gacgcggcaa cttcgagaac acgggcccga
     1441 gggcagcgcc catcaacaaa tgcctcagcc cgacgacgag cgtcgccagg acaatggtcg
     1501 cgacaggcag cggcgccgac cagagcccaa tcaccacgaa ctgcgccgcc cccgagaaga
     1561 ccatggcgct catcaaggcg gcctcgccgg cgccgaggcc ggcctgtccg gcaagcgtcc
     1621 cgaagacgag acctatggca aagccactga tcgcgatagg gacgcacctc ctcgcccctg
     1681 cgagcgcccc agaaagggta aatgtcactt tgacttctgt ctctgactta gcagtaatca
     1741 tctgtcagcg ggaatcctgg cggtctaccg ggccctctgg aaaggccggt agaacgaact
     1801 gaccagttcg gccagcagaa tcgacatcct gtgccaccca cttccgctga tctgtccacg
     1861 atgatccagg gtctctccga cgtcaaggcg ccagctacgg ccccacctgc ggtatccggc
     1921 cccgccggga taacgcgaac gtgtcgcgga gacgacgacc tcgccaacgc ccatctcctc
     1981 agccagctct cccgcgccac ggtacaacgc gcgagcaatc ggctcctcac cggcccacgg
     2041 gtccacgacg agcagcccaa gcagaagccg ctttgactcc gtcctgtaac gcagcgcgcc
     2101 aagcacctcc ccatccttcc gcgcgacgac gaacctctcc tcgaaggcga tccatcgcgg
     2161 catcccgttc aactcgagaa gttcggccac cgccgcctca tcccggacga ccccacagcg
     2221 cacctcaaca ccaccacgcc gatcttcccc ggcattccga cgagtcccgc gcaaagcacg
     2281 cgcgatcctc cgctcctcag cctcgcgcaa tagctcttcc cgccgctccc ttgctagctc
     2341 cagatcgaac ggttcaccca ccgcttgcc
//
LOCUS       DATAIR010000076         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_419691, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000076 DATAIR010000000
VERSION     DATAIR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419691"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VHM69_01930"
     CDS             <1..939
                     /locus_tag="VHM69_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2739178.1"
                     /translation="CSRNGGRVLEVAIMVEGQEGVGWDRWRRLALAVEELGYDGLHRS
                     DHFPNNGTGTYGDGLELWTSLTWLADNTERIEFGPLVSPFSFRHPVMTAWQASAVDNL
                     AGGRLRLGLGAGWNEWEHDTFGFDLLDTDRRFVRFEEGLEVVTRLLRNEEPVSFDGEF
                     YRLRDAMLLPRSPRPGGPPIVIGGNGVRRTLPLVARFADEWNAVFISAEEFVELNARL
                     DGLVDEAGRAPEQVRRTLMTRVIFGRTEADVDSKLEGEPRDQLPAAVIAGTKDEIVDR
                     LGRLAEAGVQRVMLQWLEVDDIDGLEAMAHSVLPQL"
     gene            992..1381
                     /locus_tag="VHM69_01935"
     CDS             992..1381
                     /locus_tag="VHM69_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2739179.1"
                     /translation="MISFFSILMRFVKAVRGSWRDPEFRGLAGLVIITLLAGTLFYSE
                     IEGWNLLDSLYFSVVTLTTVGYGDLSPSTATGKIFTIFYIFVGLGIILAFVNAVAERA
                     MERQSKGEDEDATEPNAANETGSPQRE"
     gene            complement(1395..2294)
                     /gene="erm"
                     /locus_tag="VHM69_01940"
     CDS             complement(1395..2294)
                     /gene="erm"
                     /locus_tag="VHM69_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HEX2739180.1"
                     /translation="MPRSGGRSTKNVANQVREIWSGKERRSRRELSQNFIRDRRIAHR
                     IVAESGAGRDDLVVEFGAGGGILTGELARVAGQVVVVEYDPYWAMHLQDRFSDRGNVL
                     VVQGDALKVKLPESPFRVVANIPFHATTPILHRLLDDPTTPLKSAHLLVQKQVALKHA
                     RSKPTTLKTLNWSPWYRFSAGLELPAHAFHPKPEVDACLMVAAKRGPPLVDPRHRYRF
                     RALVRQAFEGRGNSVGRILRPIFTRTQLRRLAKDNEFTLDLPPSMLTVRQWTNVFDFM
                     ISMVPQDHWPSPKRQARRERRRR"
     gene            2592..3038
                     /locus_tag="VHM69_01945"
     CDS             2592..3038
                     /locus_tag="VHM69_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2739181.1"
                     /translation="MADRAKLVGLNHVALEVGDIGEALEFYGRLFEIELRGRGPRMAF
                     IDAGDQFIALAEIENLSPDEHRHIGMVVDDRRIVRRALREMGVEILPGRGLDFLDPWG
                     NHWQVVDYSEIQFTKAPEVLAGMGLEGLKKSEKALEELRRKGLAPE"
     gene            3123..4232
                     /locus_tag="VHM69_01950"
     CDS             3123..4232
                     /locus_tag="VHM69_01950"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2739182.1"
                     /translation="MTGRGHPPGTPYLSVDGATMQKNILEMAEMARRNGVVLRPHVKT
                     HKVPAIAKKQVEAGAVGITVAKVSEAEVMAGEGLSDIFIAYPLVTEEKIRRAIALSKE
                     IRLIIGVDSLEGARRVSAVAGTEEHVIEVRLEVDTGLRRTGVPYEKAVELVGEIDSLD
                     NLDLTGIYTYRGAVLDGSPTLDLEAAGLQEGELMASVADRIRERGIGIQDVSLGSTPT
                     AEYAARVEGVTEIRPGTYVFYDRMQARMGACSLGDCAAEVVATVVSRPTEDLAVIDGG
                     SKTFATDVQPGGDPLNLVGFGEVVGHPEAVLGRLTEEHGMVSLRGESGIRVGDTLRII
                     PNHVCSTVNLHDALYLTGEDGTVEEVPVAARGKVW"
     gene            4254..5837
                     /locus_tag="VHM69_01955"
     CDS             4254..5837
                     /locus_tag="VHM69_01955"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX2739183.1"
                     /translation="MLKNGRVVDGTGNPWFLGDVGIKDGTIADIGRVKQEGRRTIDVG
                     GQVISPGFIDGHCHSDLMVLDTPESEIKLRQGVTTEVVGNCGMTPAPFSSENLELLRS
                     YVEPVLGTTDLTWSWERVEQYMEALLGAAPAGNVATYVGHGTLRIAVMGFENRPASSG
                     ELDRMKGLLQEALHAGAIGLSLGLMYAPGSYTPREELAELCSVLPGYDGLLATHIRGE
                     GNSLISSIEEVIWIAERCGAPLQISHLKAAGRSNWGSVTTAMDLIEDARSRGLDVTCD
                     VYPYTAGSTTLTTLLPPWALEGGVSRALERLKDPDTRARIRDELGNEQDGWDNLVAST
                     GWENVYVSSLSKGDDAGLEGKNIAEIGESRALDPVDCMMDLLLEQDGKVAIVFFHMAE
                     TDVEQVMKWDRSLIASDSLHDQAQKPHPRLYGTFPHVLATYVREKKLLTLEEAIRKMT
                     SFPARRFRLGGRGLISPGYAADLVVFDPETVSDRATYEDPKRFPEGISRVLVNGEETI
                     EMGLHRGTRAGTTIARNVP"
     gene            5863..>6199
                     /locus_tag="VHM69_01960"
     CDS             5863..>6199
                     /locus_tag="VHM69_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX2739184.1"
                     /translation="MRKTVTAEKAPKPAGPYSHAVVSGGFVFISGQGPVDPETGTMPD
                     AFADQVRQTLNNVRTILEAAGSGLDDVVKVNAYVTDLTRFQEFNEVYKEFFTYDRPAR
                     ATVGTSLLGF"
BASE COUNT         1210 a         1793 c         2075 g         1121 t
ORIGIN      
        1 tgttcgagga atgggggtcg ggtgctggaa gtcgcgatca tggtagaagg tcaggagggt
       61 gtcggatggg accgctggcg gcggctggcg ctggccgtcg aggagctcgg ctacgacggt
      121 ctccaccgct ccgaccattt ccccaacaat gggacgggta cgtacgggga cggactggag
      181 ctgtggacct cgctcacctg gcttgcggac aacactgaga ggatcgagtt cgggccgctg
      241 gtctcgccct tctctttccg ccatcctgtc atgacggcct ggcaggcctc agccgtggac
      301 aacctcgcag gcgggcgcct caggctcggc ctgggcgcgg gctggaatga atgggagcac
      361 gatactttcg gctttgatct gctcgacacg gaccgccgct tcgtccgctt cgaggagggc
      421 ctggaggtcg tgaccaggct gctcaggaac gaggagcccg tctcgttcga cggcgagttc
      481 taccggctgc gcgacgccat gctcctgccg cgctcgccgc gtccgggcgg cccgccgata
      541 gtcatcgggg gtaacggtgt gcggaggacg ctgccgctgg tagcccgctt cgccgacgag
      601 tggaacgccg tatttatctc ggctgaagag ttcgtggaac tcaacgccag gctcgacggg
      661 ctggtggacg aggctggacg ggcgccggaa caggtccgca gaaccctgat gacacgcgtc
      721 atcttcggcc gcacggaggc tgacgtggac agcaagctcg aaggggagcc gcgggatcag
      781 ctgcctgccg ccgtaatcgc gggcacgaag gacgagatcg tagaccggct cggacgcctc
      841 gccgaggcgg gtgtgcagcg ggtgatgctg caatggcttg aggtggacga catagacggc
      901 ctcgaagcca tggcgcactc cgtactgccg cagctgtgaa gctaaccacg ggagggttat
      961 agtttcctca ggctctggcg tggaggtgac tatgatctcg ttcttctcga tcttgatgcg
     1021 ctttgtaaag gccgtccgag gttcgtggcg agatcccgag ttcaggggac tcgcgggcct
     1081 cgtcatcatt acgctcctcg ccggcaccct cttctacagt gagattgagg ggtggaacct
     1141 gctcgactcg ctgtacttca gcgtcgtcac gcttacgacc gtgggctacg gtgacctctc
     1201 cccctcgacc gctacgggga agatcttcac tatcttctat atcttcgtcg ggctcgggat
     1261 tattctcgct ttcgtcaacg cggtggccga gcgcgcgatg gagcggcaga gcaagggaga
     1321 ggacgaagat gcgaccgagc cgaacgctgc caatgagacc ggctcgccgc aacgcgaata
     1381 ggcgttctct ctcatcagcg acgccgacgt tctctcctgg cctgacgctt tggtgagggc
     1441 cagtggtcct gcgggaccat actgatcatg aagtcgaaca cattagtcca ctggcgcacc
     1501 gtgagcattg aaggcgggag atccagagtg aactcattgt ctttcgcgag tctgcggagt
     1561 tgggtccttg tgaagatcgg cctgagtatc ctgcccacgc tgttgccgcg cccttcgaat
     1621 gcctgacgca cgagcgcccg gaaacgatac ctgtggcggg gatcgacgag cggaggaccc
     1681 cgtttggcgg ccaccatgag gcaggcgtca acctcaggtt ttggatggaa agcgtgcgcc
     1741 ggcagttcca ggcctgcgga gaacctgtac caggggctcc agttcagggt cttcaaggtg
     1801 gttggcttcg acctcgcgtg cttcagggcg acctgcttct gcaccagcag gtgcgcagac
     1861 ttcaagggag tggtgggatc gtccagaagt cggtgcaaga tcggggtcgt ggcgtggaaa
     1921 gggatgttcg caacgaccct gaatggactt tcagggagct tcaccttcag agcgtctccc
     1981 tgcaccacca ggacgttgcc tctgtcagag aaacgatctt gcagatgcat ggcccagtac
     2041 ggatcgtact ccacgactac gacctgccct gctaccctgg cgagctcgcc ggtaagaatc
     2101 cctcctccgg ccccgaactc cacaactagg tcgtccctcc ccgcgccgga ctcggcgact
     2161 atacggtgtg cgatccgtct gtccctgatg aagttctggc ttaactcgcg ccggctcctg
     2221 cgctccttgc cgctccatat ctcgcgaacc tggttcgcaa cgttcttcgt agatcttccg
     2281 ccacttcgcg gcatctcttg aaatcacctt cgatcttccg catgtccgat tggtggcacg
     2341 gaaagccggg cgacttctgg agagacatgg ttcagtgaga gctcagatgg agaacgctac
     2401 ctggcgggac gttctctgcc tgagtaaaga ggcgcccgac ttagtagccg cgaagccttg
     2461 tgaagcagat gttgtgagat ccgtttccca tgtacataaa ataccataga accgcgccgt
     2521 tcctatggtt ggctgcggta cgctgagttt gcacggtagc gggacgaccc tgacccgtag
     2581 ataatggggt catggctgat cgcgcgaagc tcgtcgggct caaccacgtc gccctcgagg
     2641 tcggggatat cggggaggcg ctggaattct acggacggct cttcgagata gagttgcgag
     2701 gacgcgggcc gaggatggcg ttcatagacg ccggggacca gttcatcgcg ctcgccgaaa
     2761 tcgagaatct atcccctgac gagcacaggc acatcgggat ggtggtggac gacaggagga
     2821 tcgtacgccg ggctctgagg gagatggggg tggagatcct acctggcagg ggccttgatt
     2881 tccttgaccc gtggggaaat cactggcagg tcgttgacta ctcggagatc cagttcacca
     2941 aagcgccgga agtccttgct ggcatgggcc tcgaagggtt gaagaagtcg gagaaagcgc
     3001 tggaggaact gcgcagaaag ggacttgctc cagagtaagg ccgacacgct ggtacgatgc
     3061 tcccggcagt tcctagagag gagcctcgat gctcgatacc acgaccggag taggtgtcgc
     3121 caatgacggg taggggccat cctcctggga caccctatct ttctgtggat ggcgccacga
     3181 tgcagaagaa catcctggag atggcggaga tggcccggag gaacggtgtt gtcctcaggc
     3241 cccacgtgaa gacccacaag gtaccggcga tagcgaagaa gcaggtggaa gcgggggccg
     3301 tggggatcac ggtggccaaa gtctccgagg cggaggtcat ggccggcgaa gggctctccg
     3361 acatcttcat cgcctatcct ctcgtaacgg aggagaagat ccggcgcgcc atcgccctga
     3421 gcaaagagat caggctcatc atcggggtgg acagcctcga aggggcgcgg agggtctccg
     3481 ccgtcgcagg gacggaagag cacgtcatcg aggtcaggct ggaggtggac acagggctca
     3541 ggagaacagg tgtcccatac gaaaaggcgg tcgagctcgt cggcgaaata gactccctgg
     3601 acaacctgga cctgaccggg atctacacgt accggggagc cgttctcgat ggctcgccga
     3661 cgctcgacct cgaagcggct ggtctgcagg agggcgagct catggcctct gtggcggacc
     3721 ggatcaggga acgggggata ggcatacagg acgtcagcct cggatctacc ccgactgccg
     3781 aatatgcggc aagggttgag ggtgtgacgg agatccggcc cggcacctac gtcttctacg
     3841 acaggatgca ggcgcggatg ggcgcatgct ctctcggaga ctgcgccgcc gaggtggtcg
     3901 cgacggtggt cagcaggccg acagaagacc tggccgtcat cgacggggga agcaagacct
     3961 tcgccaccga cgtacagcct ggaggcgatc ccctgaacct cgtcgggttc ggcgaggtgg
     4021 tcgggcaccc ggaggcggtt ctggggaggc tcaccgagga gcacggcatg gtctcgctgc
     4081 gcggggagag cggcatcaga gtcggcgata cgctcaggat catacccaac cacgtttgca
     4141 gcaccgtcaa cctgcacgac gcgttgtatc tgaccggtga ggacgggacc gtggaagagg
     4201 tgcctgtggc ggcccgcggg aaggtctggt aggaagaacc tgtgttggac ctcgtgctga
     4261 agaacgggcg cgtcgtggac gggaccggca acccctggtt cctcggcgac gtggggatca
     4321 aagacggcac catagcggat attggaaggg tgaagcagga aggcaggcgg accatcgacg
     4381 ttggcgggca ggtgatctca cccggcttca tagacggcca ctgccactcg gacctcatgg
     4441 tcctcgacac gccggagagc gagatcaagc tcaggcaggg tgtcaccacc gaagtcgtcg
     4501 gaaactgcgg aatgacgccc gcgccattct cgtccgaaaa cctggagctg ctcaggagct
     4561 acgtggagcc cgtgttaggt acgacggatc tgacgtggtc ctgggagagg gtcgagcagt
     4621 acatggaggc tctgctcggg gcggcgcccg cagggaacgt agccacctac gtcggccacg
     4681 ggacgttgag gatagcggtg atgggtttcg agaacaggcc cgcttcgagc ggagagctgg
     4741 accgcatgaa gggccttttg caggaagccc ttcatgccgg tgcgataggt ctctcccttg
     4801 gactgatgta cgccccaggt agctataccc cgagggagga gctggcggag ctgtgttccg
     4861 tgctccctgg ctatgacggg cttctcgcca cccacatcag gggcgaaggg aacagcctca
     4921 tctcctccat cgaggaggtg atctggatcg ccgagcggtg cggtgcaccg ctccagatca
     4981 gccacctcaa agccgcgggg aggagcaact gggggagcgt aaccacggcg atggatctga
     5041 tagaagacgc gaggtccagg gggctggacg tcacctgcga cgtatacccg tacactgccg
     5101 gctcgaccac cctgaccacg ctgctcccgc cgtgggcgct ggagggtggc gtctcaaggg
     5161 cactggagag gctgaaagat ccggacacac gggcgaggat cagggatgag ctgggaaacg
     5221 aacaggacgg ctgggataac ctcgtggcct ccacgggttg ggagaacgtc tacgtctcct
     5281 cactcagcaa gggtgacgat gctggcctcg aaggcaagaa catcgccgag atcggcgagt
     5341 cgcgggcgct ggatcccgta gattgcatga tggacttgct tctggagcag gacgggaagg
     5401 tcgcgatcgt gttcttccac atggccgaaa ccgacgtgga gcaggtgatg aagtgggacc
     5461 ggtcactaat agcctccgac agcctccacg accaggccca gaagccccac cctcgtctgt
     5521 acgggacctt tccgcacgtc ctcgcaacgt acgtgcggga gaagaagttg ctgaccctcg
     5581 aagaggcgat caggaagatg acctccttcc cggcccggcg attcaggctg ggcgggcgcg
     5641 gtctgatctc ccctggatac gccgccgacc tggtcgtctt cgacccggag accgtctcgg
     5701 acagggccac ctacgaggac cccaaacgct tcccagaagg tatctcccgc gtcctggtca
     5761 acggagagga gacaatagag atgggtctcc atcgggggac gagggcgggt acaacgatag
     5821 caaggaacgt tccttgagca cgacgtccac aaaggaggat ctatgagaaa gaccgtaacc
     5881 gccgagaagg ccccgaaacc ggcgggtccg tactcgcacg ccgtggtatc tggcggcttc
     5941 gtgttcatct cggggcaggg tcccgtagac cccgagaccg gcactatgcc cgacgccttc
     6001 gccgaccagg ttcgccagac gctaaataac gtgcggacga tcctggaggc cgctggctcc
     6061 ggcctcgatg atgtcgtcaa ggtgaacgct tacgttacgg atctcacgcg gtttcaggag
     6121 ttcaacgagg tgtacaaaga attcttcacc tacgaccgtc ccgcccgtgc caccgttggc
     6181 acctcgctcc tcgggttcc
//
LOCUS       DATAIR010000077         9743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_419699, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000077 DATAIR010000000
VERSION     DATAIR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419699"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VHM69_01965"
     CDS             <1..912
                     /locus_tag="VHM69_01965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2739185.1"
                     /translation="YGTADEDESIATIHRAIELGITFLDTADMYGPFTNERLVGKAIS
                     GRRDEIVLATKFGNERREDGSWVGVNGRPEYVRSACDASLDRLGVDHIDLYYQHRVDS
                     ETPIEETVGAMKELVEAGKVRYLGLSEAGTETIRRAHAVHPISALQSEYSLFTRDVED
                     EVLPTVRELGIGFVPYSPLGRGFLTGRWRSIEDMPEDDTRSARFPRFAEVNFKKNLEL
                     ADRVREAADEKGVTPGQLALAWLLAQGDDIVPIPGTKRRKYVEENAGAASITITEEDL
                     SRIEEAMPRGSAAGERYTEEQMRSVGR"
     gene            complement(1177..2334)
                     /locus_tag="VHM69_01970"
     CDS             complement(1177..2334)
                     /locus_tag="VHM69_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739186.1"
                     /translation="MSKTVLLLAMAATTLLMAGCSDPTAQRNEADRQSPENQNGTPKQ
                     EKTVVIVKNEGMSKKEEEELQQRLDDLEKKVEDKSQQDTENQSPSKPEQDAKSTEEQA
                     SKAAQDYYAAAAAGDYNYTYDALSSASQGQLTEEEWVAANTALGSDAGIYHIDATNVV
                     DDSTVVVYLTITLADGSSSERTTEFVLENGSWKHDLTQSEYELFAGAIASAPASATAP
                     SSDTDSSTGPNFGDGTYQVGTDIQPGTYRTREGSPGCYYERLSGFSGEFDDILANGNT
                     DAPAVITIKPTDAGFTSQGCGTWTKDLSTITESKTSFGEGAYIVGTDIEPGTYGNSGS
                     SGCYYERLSEFTGDFDAIIANGNTNASAVVTIAPTDAGFQSQGCGTWSKLE"
     gene            complement(2648..3235)
                     /locus_tag="VHM69_01975"
     CDS             complement(2648..3235)
                     /locus_tag="VHM69_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739187.1"
                     /translation="MRKVVLLLASMAIAILVVTGAALAATINGTAKDDHLEGTPKADT
                     ISGRAGNDYIEGLAGDDLLIGGRGNDTLWERFGDDKLYGWGGDDTLIGWKGYDRLDGG
                     DGHDKLVNGDEMFGGGGPDRIGDAVWNQPQGGSFSAYGGAGDDRINAEDHHFEVQDGI
                     STPDTVECGAGEDTVKAEADDTVANDCEHVEIVVH"
     gene            complement(3639..4166)
                     /locus_tag="VHM69_01980"
     CDS             complement(3639..4166)
                     /locus_tag="VHM69_01980"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2739188.1"
                     /translation="MRRSTAAVGSALFFLLAPGVVVGLVPWALTRWQVREPLPYWAPA
                     RVLGGVVLVAGLIALVQAFVRFVVEGRGTPAPVAAPERLVVGGAYRHVRNPMYVAVLA
                     AIVGQALLLGRLGLLVYAGGTWLVVAAFVRFYEEPVLARRFGADYEAYRRAVPAWWPR
                     LRPWEPGGPDEPGAE"
     gene            complement(4683..5009)
                     /locus_tag="VHM69_01985"
     CDS             complement(4683..5009)
                     /locus_tag="VHM69_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739189.1"
                     /translation="MRVFVLVEPKFFTPPEMFPALLDGFAAWRDAHREHMDSFEFFAG
                     GGGGFGILNVPDEATLNRIMVQFPFTPYSEITSRPILDGDTALGQWREIMSELMGAGP
                     GVEPSQ"
     gene            5585..5839
                     /locus_tag="VHM69_01990"
     CDS             5585..5839
                     /locus_tag="VHM69_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739190.1"
                     /translation="MLQLISVLGAFAILAAYAANLFGIIQPSNLWYSVANFLGSAVLT
                     VVAVVDRQLGFLLLEGTWALVSLWGIFSVLRGRGAPPDSH"
     gene            5878..6078
                     /locus_tag="VHM69_01995"
     CDS             5878..6078
                     /locus_tag="VHM69_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739191.1"
                     /translation="MAGVKYVSNVKVEPVEGKIRRAYLPVQKEPVLFGVHSEVAEHYG
                     VSPEDVEPYDTTLDYVVAAAGG"
     gene            6079..6342
                     /locus_tag="VHM69_02000"
     CDS             6079..6342
                     /locus_tag="VHM69_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2739192.1"
                     /translation="MLGTFSGALAARQVTFENLHGQTVGEIEVESKVLVIKRIKQVFH
                     LTAKEQDRETIERVLRVYADSCPVAASFKGSIEISSELDLTTG"
     gene            complement(6353..7825)
                     /locus_tag="VHM69_02005"
     CDS             complement(6353..7825)
                     /locus_tag="VHM69_02005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2739193.1"
                     /translation="MTEINAMPEEPIALVTVSADTGFLPPVIHFVKQMSDRLGLGDAA
                     AEHMDRAIELVCRNVIQHAFGPGEEGSYDIYVLRGPGQVIVAVEDQGLPFDYSRLQEG
                     KDRTLLEIVHRSFDEIRFKNLGRHGNRVELIKHLPHTDVRDHLPEDEHRRTVEAAEED
                     GPLEIRMMRPEESIELSRCVYRSYGYSYDWDYIYYPDRIRELQEKGLMRSCVAVTPGG
                     EFAGHLAITVERPDSPVGEAGQAVVDPRFRGHHLFPKMKTFMAERAGNAGMYGLYSEA
                     TAVHPYSQRGNLQLGAKETGFLLGYIPASVSYKQIGENREGRRGSVALFYMRVNDEPE
                     REIHPPTAYREEIQAVIEHNGLRRVIADGVGPQMSSLSRMSVEVRKDHNLAFVRVEEP
                     GADLAELVDRHLRDLCLHRVDCVYVDLPLSHPATRRAGLKNLGVFFGGIIPEAHAGGG
                     DVLRLQYLNNVEVEPDDVSTASDFGHELLGLIFRQKNALT"
     gene            8399..8620
                     /locus_tag="VHM69_02010"
     CDS             8399..8620
                     /locus_tag="VHM69_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739194.1"
                     /translation="MFNDWQYMVLQDRTAREQDFAQRKAERGKYARSPGAPSLRARVA
                     RRLFDLAVAAEREETWRVVWEKLEAKGRL"
     gene            8663..9448
                     /locus_tag="VHM69_02015"
     CDS             8663..9448
                     /locus_tag="VHM69_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739195.1"
                     /translation="MKALELRGVEKSFKKRRGEVVRALDSVSLSVGKGEVVGILGPNG
                     SGKSTLVRVISTLVLPDSGEVEVFGVDAVRHPKHVQRCMNRVSVEASFFKKLSAMENL
                     LYGAKLYGVTSAEARPRIKEILENIGFARKRATEPMEHLSRGMQQKIALARALLTSPV
                     LMLLDEPTTGLDPRSKKDVQAFIERIRDAHDSSILLCTHDMGEAEELCDRVGIMVDGK
                     ILALEEPEDLKRRYATNGRVPTLEETFMAATGYSVEEASADTE"
     gene            9457..>9743
                     /locus_tag="VHM69_02020"
     CDS             9457..>9743
                     /locus_tag="VHM69_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739196.1"
                     /translation="MRGNVKPIWLSEMVAVWGYVQRNYFLTKRYFMWEVVWLVYVTIN
                     AMAITFIGAGVEAAGGTVDKEFLMTYLLVGALLWNYLSMLFDVLSETVSWE"
BASE COUNT         1905 a         2947 c         2973 g         1918 t
ORIGIN      
        1 tatggtacgg cggacgagga cgagtccata gctaccatcc accgcgcgat agagctggga
       61 ataaccttcc tcgacaccgc ggacatgtac ggtccattca ccaacgagag gctcgtaggc
      121 aaagctatct ccggtcggcg cgacgagatc gtcctcgcga ccaagttcgg aaacgagcgc
      181 cgcgaggacg gatcctgggt gggagtaaac ggcaggccgg agtatgtgag aagcgcctgc
      241 gacgcctcgc tggatcggct cggtgtcgac cacatcgacc tctactacca acacagggtc
      301 gactctgaga ctcccataga ggagacggtg ggcgcgatga aggagcttgt cgaggccggc
      361 aaggtgcgct acctcgggct ctcggaggcg gggaccgaga ccatccgcag ggcccacgcc
      421 gtgcatccca tcagcgctct ccagtcggag tactcgctct ttacccgcga cgtggaggat
      481 gaggtactgc cgaccgtgag ggagctcggc atcggcttcg tcccatacag cccgctcgga
      541 cgcggcttcc tcacaggacg ctggcgcagc atcgaggaca tgcccgagga cgacacgcga
      601 agcgcccgct tcccgcgctt cgctgaagtg aacttcaaga agaacctgga actggcggat
      661 agagtacgcg aggccgccga cgagaaggga gtgaccccgg gccagctcgc cctggcgtgg
      721 ctgctcgccc agggcgacga catcgtcccg atcccaggca ccaagcgtcg caagtacgtc
      781 gaagaaaacg ccggcgccgc cagcataacg atcaccgaag aagacctctc ccgcatcgaa
      841 gaggcaatgc cgcgcggatc agccgccggc gagcgctaca ccgaggagca gatgcggtcc
      901 gtgggccgct aaggaggagt ccgtgaccga cgtcgagcac atctggcgta cccataagct
      961 ggttccccgt ccctgaggag tccaagctgc ctgatgagtt gcagggtctg tttcgcaagg
     1021 cgcgggaaaa agtcgggtta gtccccagcg tcttcagggc gtacagttat cgtccagagc
     1081 ggctctccgc ctggttcgcc cataacaggc acactgagca tctgcctacc ggaagcgtaa
     1141 ttaccacgcc gggaagaccg ccgaattgca ggcactttac tcaagtttcg accacgtacc
     1201 gcaaccttga gattggaagc cagcgtctgt gggcgcaatg gtcactactg ccgatgcatt
     1261 ggtattgccg ttagctatta tggcgtcaaa atctcccgtg aattcactca gtctttcgta
     1321 gtaacaaccg cttgagccgc tgttcccata ggtcccaggt tcaatatctg tgcccacaat
     1381 gtaagcaccc tcaccaaagg atgtcttgct ctctgtgatt gtagatagat ccttagtcca
     1441 agtaccgcac ccttgggacg tgaagccggc atcggttggc ttgattgtga ttaccgccgg
     1501 agcgtctgta ttaccattgg caagaatatc gtcgaactca ccactaaagc cgctgagacg
     1561 ttcgtaataa catcctggtg aaccttccct ggttcgataa gttccgggtt gtatgtccgt
     1621 tcctacctgg tatgtgccgt cgccaaaatt aggacccgta gaggagtcgg tgtcagagga
     1681 aggagcagtg gcggaggcag gcgcggaagc aatggcaccg gcgaagagct catactcgct
     1741 ctgggtaagg tcgtgcttcc agctaccgtt ctccaggaca aactctgtag tacgctcgga
     1801 actagaaccg tccgccaggg taatggttag atagactacc accgtcgagt cgtccaccac
     1861 attggtcgcg tcgatgtgat agattcctgc gtcactaccc aaggcggtat tggcggccac
     1921 ccattcctct tcggtgagct ggccctggga agcagaggag agcgcatcgt acgtgtagtt
     1981 gtagtcgccc gcggcggcag ccgcgtagta gtcttgggcc gccttcgagg cttgctcttc
     2041 tgtactcttt gcgtcctgct caggcttact tggactctga ttctcagtat cctgctggga
     2101 cttgtcttca accttctttt ccagatcgtc caagcgctgt tgaagctcct cttcttcctt
     2161 cttgctcatg ccttcgtttt ttacgattac aaccgtcttt tcttgtttag gagtgccgtt
     2221 ttgattctcg ggactctgtc gatcagcctc gttcctctgc gctgttggat cactgcatcc
     2281 cgccatgagt aatgttgttg ctgccatagc caacagcaga actgtcttgc tcatactgat
     2341 ataccctccc ttagccggga agaaagcccc acctcattgc acgacgtatt gaagcctacc
     2401 ctggaactca gaaaggcgtc ggcggcctat aagagagtac gagatggacc aaagtggaag
     2461 aggcatcggc caggcggcac atcttcctgt ataccaggca atgggccgga gccccggccc
     2521 tcgcgataca cctgttcgcc cgttctagta gcggatcacg tcctcgcagg agtcgtctac
     2581 gaagtcccga acgctggtgt cgtcggcctg atccttgctc gcggtcggct ccgtgggggc
     2641 gctctcctta gtgcactacg atctccacgt gctcgcagtc gtttgccacc gtgtcgtcgg
     2701 cctcggcctt gaccgtgtcc tcgccggctc cgcattcgac cgtgtccggt gtgcttatgc
     2761 cgtcttggac ctcgaagtgg tgatcctcag cgttgatacg gtcgtcgccg gcgccgccgt
     2821 aggctgagaa gctgccgcct tggggttggt tccagaccgc gtcgccgatc ctgtccgggc
     2881 cgccgcctcc gaacatttcg tcgccattga cgagcttgtc gtggccgtcg ccgccatcga
     2941 ggcggtcgta acccttccag ccgatgaggg tatcgtcgcc cccccagccg tagagcttgt
     3001 cgtcgccgaa gcgttcccag agcgtgtcgt tacccctccc gccgatgagc aggtcgtcgc
     3061 cggccaggcc ttctatgtag tcgttgccgg ccctgccgga gatcgtgtcg gccttgggcg
     3121 tgccctcgag gtggtcgtct ttcgcggtgc cgtttatcgt cgcggccagc gccgccccag
     3181 taactaccag gatcgctatt gccatcgagg ccaataggag aacaactttt ctcatctcca
     3241 ctacctttct ttagtgtggt ggagaagctt ggcaacgctc caccctgtta agcggcgaat
     3301 agatcatata ttgcggcaag ctggaaagaa tgtgaccagt gtccctattt tgggggtcat
     3361 gtggatactt cttctagaac atctcccttg caaacatctt acagcggctt gcggaaggat
     3421 tgatcctccc caagctgggc atcaggcatc aggaaggtct tgccggacgg tggtcgctaa
     3481 ggtgcacctc tggggcgagg tgggtcaata cttatgaacc gcgctgttag aactccgtca
     3541 agagaccgtc tgaaaatcct caggtaccca gcctccctac catgcccctc gtgggcctcg
     3601 gtcggatgcg gaagccctgc ctcggtgggg agggagcttc attccgcgcc tggttcgtcg
     3661 ggcccgcccg gttcccacgg acgcaggcgg ggccaccagg cgggcacagc gcgccggtag
     3721 gcctcgtagt ccgccccgaa gcggcgggcg agcaccggtt cctcgtagaa gcgtacgaac
     3781 gccgcgacga ccagccaggt gcccccggcg tacaccagca ggccgagccg gcccaggagt
     3841 aacgcctggc cgacgatggc cgccagtact gccacgtaca tcgggttgcg gacgtggcgg
     3901 tacgccccgc cgacgaccag acgctcgggc gcggcgaccg gtgccggcgt tccgcggccc
     3961 tccacgacga agcgtacgaa cgcctggacc agcgcgatca gtcccgcgac gagcaccacc
     4021 ccgccgagca cccgcgcggg cgcccagtac ggcaggggct cacgcacctg ccaacgcgtc
     4081 agcgcccacg ggaccaggcc cacgaccacg cccggggcca ataagaagaa cagcgcgctt
     4141 cccaccgcgg ccgtcgatct gcgcatagta acgcctcacc tcccagtcac cagcataccc
     4201 gagtggccgc tttcggcccc gcccacgttc gctgccggcg aggcggttac gtacaagccc
     4261 tgttcgccca accgcgtggg tagaccgtcc gcaaactctg aatcacgcgc atagatcgga
     4321 ccgtagacga tacgctgatg cttcttcaga cggtctcaag gcgaagttct gagaagatct
     4381 cgactacgac tatcgctgat gtagcgcccg ctctctcgct acccggccgg tccgtcgcct
     4441 cagtacccgg gtgtaaccgc ctgggcccgt gcttcgtcag aagcggtacg ggcccacgtt
     4501 ccgaaaagag aggctaacgg acgtgaaagc tacccttcaa agaggccgtg ggcccgcagc
     4561 ctcaatgtat caaagcgtga tctcgccggg catgtgtcaa ttgacctagc ttctccgcac
     4621 agacgcaccc cgatagggcc gggagatagc cccggtcagt tacgcagatg gttaatgctc
     4681 tcttactgac tcggctccac tccaggaccc gcgcccatca actcgctcat gatttcccgc
     4741 cattgcccta acgctgtatc cccgtcgagt atggggcgag acgttatctc gctgtaaggc
     4801 gtgaacggga actggaccat tattcggttc agggttgcct cgtcgggcac gttcaagata
     4861 ccgaagcccc cgccgcctcc ggcgaagaac tcgaaggaat ccatatgctc tctgtgagcg
     4921 tcccgccagg cggcgaatcc gtcaagtagt gcggggaaca tctccggcgg agtgaagaac
     4981 ttcggttcga cgagcacaaa cacacgcata agtctatgcc tccttttctc gttgcctttc
     5041 cctgccagta tgatgccacc gcatgtaagc tagcgcatcc cccaagtgat ccagattgac
     5101 tgcggataag aagaagggcc gagactccgc actaagcccc ggcccgatat gatctcacac
     5161 gctaggctgg tagtgtgcct gccctaaagc gctccagctc ctcatccacc tgatctttaa
     5221 cctcgtagag ctcagaggaa gttggagttt ctgcgccgtt acgaggagga gatggcgcaa
     5281 ctagagcagg acagggacac catattggag agctacgccg gtgcctccga ggaggccctc
     5341 gattcgctca cgcccgagca gcgccacaat ctctgcaagt catttcggat agaggtcctt
     5401 gctcacgctg acggcactac cgagatcgtg ctgggagacc tgttaagcta cgaagaggtt
     5461 cgtacaaagg agtcgttatc gagatcgcaa gcatgtacaa ctttaccaac cgcacgtcgc
     5521 tcgcgtgcgg catgttcccg aacgaggaat accacgctct ggcgagatag agaggtatct
     5581 gtccgtgctg cagttgatct cggttctcgg ggcgttcgcc atcctcgcgg cctacgcggc
     5641 caacctgttc ggtattattc aaccctccaa cctgtggtac tcggtggcca acttcctcgg
     5701 ctccgccgtg cttacggtgg ttgcggtagt ggaccgtcaa ctcggctttc tactcctcga
     5761 aggcacctgg gctctggtga gcctctgggg gatcttctct gtcttgcgcg ggaggggagc
     5821 gccaccggac agtcactaga catctgtata ctgcgtgaaa gcggagagga ggagaacgtg
     5881 gccggagtga agtacgtctc gaacgtgaag gtggagccgg tggagggtaa gatccgccgc
     5941 gcttatctcc ccgtccagaa agagcctgtg ctcttcggtg tccacagcga ggtcgcggaa
     6001 cactacggcg tctccccgga ggacgtagaa ccctacgata cgacgctcga ctacgtggtc
     6061 gccgcggcgg gcgggtgact gctcggtacc ttttcaggcg cgctggcagc gcgtcaggtg
     6121 acgttcgaga acctccacgg ccagacggtc ggagagatcg aagtcgagag caaggtgctc
     6181 gtgatcaaga ggatcaagca ggtcttccac ctcaccgcga aagaacagga ccgcgagacc
     6241 atagaaaggg tgctgagggt ctacgcggat agctgtcctg tcgcggcttc gttcaagggg
     6301 agcatcgaga tatccagcga gctcgacctg acgaccgggt agcccggcgc tgttaagtca
     6361 gggcgttctt ctgacggaag atcaggccca gaagttcatg cccgaagtcc gaggcggtgc
     6421 tgacgtcgtc gggctcgacc tcgacgttgt tcaggtactg gagacggagt acgtcgcctc
     6481 ctccggcgtg ggcctcgggg atgatcccgc cgaagaagac gccaaggttc ttcagcccgg
     6541 cccgccgcgt cgcgggatgc gagaggggaa ggtccacgta gacgcaatcc acccgatgca
     6601 ggcagaggtc ccgcagatgg cgatctacca gctccgccag gtcggcccct ggttcttcca
     6661 cccgcacgaa ggccaagttg tgatccttac gcacctcgac gctcatcctg gataacgacg
     6721 acatctgtgg cccgacgccg tctgctataa cgcgccgcag cccgttgtgt tcgatgacgg
     6781 cctggatctc ctcccggtat gcggtgggag ggtggatctc ccgctccggc tcgtcgttga
     6841 cgcgcatgta gaaaagggcg acggagccgc gacgtccttc tctgttttca ccgatctgtt
     6901 tgtatgagac ggaagccggg atgtagccaa gcaggaaccc cgtctccttc gcgccgagtt
     6961 gcaggttgcc cctctggctg tacggatgga cggcggtcgc ctcgctgtag agcccgtaca
     7021 ttcctgcgtt cccggcccgc tccgccatga aggtcttcat cttcgggaag aggtggtgcc
     7081 ccctgaaccg tgggtccacg accgcctgtc ctgcctcccc gacgggcgag tcggggcgtt
     7141 ccacggtgat ggcgagatgt cccgcgaact cgccccccgg cgttacagcc acgcacgagc
     7201 gcatcagacc tttctcctgg agctccctga tcctgtcagg atagtagatg tagtcccagt
     7261 cgtagctgta gccgtaggat cggtagacgc agcgcgaaag ctcgatggat tcctcgggac
     7321 gcatcatgcg aatctcgaga gggccatctt cttcggcggc ttcgacggtt ctgcggtgct
     7381 cgtcctcggg gaggtggtcc ctgacgtcgg tatgagggag gtgcttgatg agctcgaccc
     7441 tgttgccatg gcgaccgagg tttttgaagc ggatctcgtc gaatgacctg tgcactatct
     7501 ccagcaaggt ccggtccttg ccttcttgca ggcggctgta gtcgaagggc agcccttgat
     7561 cttctacagc cactatcacc tgccctggcc cgcgcaggac atagatgtcg taagatccct
     7621 cctctcccgg accgaatgca tgctgtatga cgttccggca gactagttcg atggcccggt
     7681 ccatatgctc ggcggccgcg tcaccgaggc cgagccgatc gctcatctgc ttcacgaagt
     7741 ggatcaccgg cggcaggaac ccggtgtcgg cggagaccgt gacgagggcg atcggttcct
     7801 ccggcattgc gttgatctcg gtcatagcaa ttctctcctc acaaagacga actccatccg
     7861 gattttgatc ttctcaggtc ttgctgacat ctgctatagg ggtgccgtgt gacaggcgac
     7921 ggtgtctcgt caaacacccg ggattctcag acgaggttgt caccttcgac agacgcgtcg
     7981 ccgtggcgcg cgtgcttcag gggtcggacg acaacatcag gaaagtttcg caagtgatcg
     8041 atgcgcaggg cctcatcagg tgacaacatc tcgaatggca aaatttgtgt tgacagggtt
     8101 ctcatgggag ggtagagtgc taacgttatg gtagtcgcga aaggacctga ggtattgatg
     8161 cacgtcaatc gcacgaacat aggagttccg gtgttcgggg gagaggtgcg tcccgaggtc
     8221 cgtgtggtcg cgatgagaac gtgagcgtct agctcgacaa cacttctcaa acgaggcccc
     8281 cggaccgacc ggaccggggg cctcaagctt tgctcgaaga gcgaaggtct ccgatcccgg
     8341 tacggacagt atcagccact ttcaggcgcg accaggagat gagaggagat cgaaaacgat
     8401 gttcaacgac tggcaataca tggtgttaca ggaccggacg gcccgcgagc aagacttcgc
     8461 ccagcgaaag gccgagcgcg gaaaatacgc gcggtccccc ggagcaccct ccctgagggc
     8521 cagggtggca cgccggctct tcgacctggc ggtagccgcc gagcgcgagg agacctggcg
     8581 cgtcgtctgg gagaagttgg aggcgaaggg gcgcctgtaa ggcgggcgcc cctcagggcg
     8641 gaggtataga ggaggttcac atatgaaggc tttagaactt agaggcgtag agaaaagttt
     8701 caagaagcgg cggggggagg tggtgcgcgc cctcgactca gtctcgctct cggtcggaaa
     8761 gggggaggtc gtcggcatcc tcgggcccaa cgggagcggg aagagcacgc ttgtgcgtgt
     8821 gatctccacg ctcgtcttac cggatagcgg ggaggtcgag gtcttcggcg tggacgccgt
     8881 gcgccatccc aagcacgtgc agcgctgtat gaacagggtc agcgtcgagg ccagcttctt
     8941 caagaagctc tcggccatgg agaacctgct ctacggcgcc aagctctacg gagtcaccag
     9001 cgccgaggcg cggccaagga taaaggagat cctcgaaaac atcggctttg cgcggaagcg
     9061 ggccaccgag cccatggagc atttgagccg ggggatgcag cagaagatcg cgctcgccag
     9121 ggcgctcctc acgagccccg tcctcatgct cctcgacgag ccgacgacgg gcctcgaccc
     9181 caggagcaag aaggacgtgc aggcgtttat cgagaggata cgcgacgccc acgactcctc
     9241 gatcctcctc tgtacgcacg atatgggcga ggccgaggag ctgtgcgacc gcgtcggcat
     9301 catggtcgac gggaagatcc tggcgctcga agaacccgag gatctgaaac ggcgctatgc
     9361 cacgaacgga agggtgccga cgctggagga gacgttcatg gccgcgaccg gctactccgt
     9421 cgaggaggcc tcggcggata cggaatagga gaaaagatga gaggcaacgt gaagcccatc
     9481 tggctatccg agatggtggc cgtctggggc tacgtacaac gaaattactt tctgacaaag
     9541 cgctacttca tgtgggaggt ggtgtggctc gtctacgtga cgatcaacgc catggcgatc
     9601 acctttatcg gtgccggggt ggaggccgcc ggcgggacgg tggacaagga gttcctcatg
     9661 acgtacctgc tcgtgggcgc gctcctgtgg aactacctct cgatgctctt cgacgttctc
     9721 tcggagacgg tgagctggga gag
//
LOCUS       DATAIR010000078         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_4604, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000078 DATAIR010000000
VERSION     DATAIR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4604"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VHM69_02025"
     CDS             <1..563
                     /locus_tag="VHM69_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2739197.1"
                     /translation="MQVLRRRPEGVDRAGETSRQIEALKRAVGGLRDAIYDLRLESDR
                     ERPLVRAVQSIVELNRQISDGCEFELVVDEGFPATISGAAGIEVVRVVQEALTNVRRH
                     SAASHAAVTLGVVNGDVLVEIEDDGRGFGPETSYGMGLTGMRERVHMLGGKLDVEGRE
                     GRGTRVFLRVGLAVLTGGGTGELSNT"
     gene            644..1297
                     /locus_tag="VHM69_02030"
     CDS             644..1297
                     /locus_tag="VHM69_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739198.1"
                     /translation="MQRILLVEDHASFRQTLAFVFDQQPEFEVVAQAGTLAEARRTMG
                     CFEADLGVIDLSLPDGEGTELIEELREATPHFAALVLTASLDRAEHARAVEAGAAGVL
                     HKSADVDEILDATRRLGEGETLLSQDELVALLRLAGQNREEELEARASIEQITPRERE
                     VLQTLAEGLSNKEIAAKLHMSVDTERTHMMNILNKLGVHSRLQALLFAARHGIVEIR"
     gene            complement(1344..2156)
                     /gene="dapF"
                     /locus_tag="VHM69_02035"
     CDS             complement(1344..2156)
                     /gene="dapF"
                     /locus_tag="VHM69_02035"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX2739199.1"
                     /translation="MHFTKMQGCGNDFLIFDPREVEGVDLPELALRACDRHFGVGADG
                     ILVPSPSEAADIEMVYLNSDGSRSEMCGNGLRCLARYARDRVMVEGEAITVATGAGVK
                     KVILFEDGSSRVDMGAPGFDTEIDVGGFRFLRVSMGNPHAVTFLESEAAVETLDLRAV
                     GPPIENDPVFPNKTNVEFGFARGEREVRMRIWERGAGETLASGSGSCAVAVAAVKLGL
                     AESPVSVRLDGGIVEIEWSGEGEPVYMKGPAEYVCEGELSLSREKEVLTTDT"
     gene            complement(2063..2641)
                     /locus_tag="VHM69_02040"
     CDS             complement(2063..2641)
                     /locus_tag="VHM69_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739200.1"
                     /translation="MGGRCPSGDKGQRSPLPRGRAHLGELDERRAEAEDELAALRVGQ
                     RRLDDLRSYATLIDEHLRELPDLVHGRDDAIREHTYTEEHEECRREAAAEGRLPVFRP
                     SPNMFRERTPEEMEELRRVRERERAERYRGVYTTLGLRIVAHEDGTLELTWRAGEGVS
                     ERCTSPRCKAAAMIFSSSTPARSKVSTCPNSL"
     gene            complement(2740..2934)
                     /locus_tag="VHM69_02045"
     CDS             complement(2740..2934)
                     /locus_tag="VHM69_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739201.1"
                     /translation="MPTPGGGRYWHAGTIREMICNDVYRAHGRDELERFVETGNLSPE
                     VLAGLDRDARYGIAWYNRTR"
     gene            3523..>4505
                     /locus_tag="VHM69_02050"
     CDS             3523..>4505
                     /locus_tag="VHM69_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739202.1"
                     /translation="MTVRGLMVLAVFAVLYSCGQASSPVENQEKKQGVVEQARREVPE
                     APSIGCALFPDDSYWHADVSNLPVHPESEAFIDAIGRDTPLKADFGAGLYEGSWIGIP
                     YTGVDGDQPRVDVAFRYDEESDPGPYPIPSDAPLEGGPEFVKDNDRHVLVVERDSCTL
                     YELYKAESKGDGTWKAGSGAVFDLDSNDLRPETFTSADAAGLPILPGLVRYDEVASGE
                     IDHPIRFTLEETRDQYVWPARHEAGQPGADLPPMGLWLRLKADFDTSGFPEELQVILR
                     AMQKRGLILADNGSSMFISGVPDNRWDNDTLARLREIDAGDFEAVDVSSLKV"
BASE COUNT          788 a         1468 c         1447 g          802 t
ORIGIN      
        1 cgatgcaggt tctcaggaga aggccggagg gcgtcgaccg cgccggagag accagccgcc
       61 agatagaggc gctcaagagg gctgtggggg gcctgcgcga cgccatctac gacctgcggc
      121 tcgaaagcga cagggagcgg ccgctcgtcc gcgccgtgca gtccatcgtc gagctgaacc
      181 gccagatcag cgacggctgc gagttcgaac tggtcgttga cgaggggttt ccggcaacga
      241 tctcgggggc cgctggtatc gaggtggtgc gcgtcgtcca ggaagcactg acgaacgtca
      301 ggcgccactc ggcggcctcg catgccgcgg tcaccctcgg tgtggtgaac ggcgatgtcc
      361 tcgtggagat cgaggacgac ggcaggggtt tcggccccga gacgagttac ggtatggggc
      421 tcacggggat gcgcgagcgc gtccacatgc tcggtggaaa gctcgatgtg gagggcaggg
      481 agggcagggg aaccagggtg tttctcaggg tggggcttgc cgtcctgacg ggcggcggaa
      541 ccggagagct ctcgaacacc tgagacggcg gtctccaggc cgtcaaagct ggtatattgt
      601 tttcgagcgt ggaagtgcaa gagaggtccg cgagagggag gaaatgcagc gaatcttgct
      661 tgtagaggac cacgcctcgt tccgccagac gctcgcgttt gtctttgatc agcaacccga
      721 gttcgaggtg gtcgcccagg ccgggacgct cgccgaagca cgccggacga tgggatgctt
      781 cgaggcagac cttggagtta tagatctctc tctacctgat ggggagggca ccgagctgat
      841 cgaagagctg cgcgaagcca ccccgcactt cgctgcgctc gtccttaccg cgagcctgga
      901 cagggctgaa cacgccaggg cggtcgaggc aggggcggcc ggcgtcctgc acaagtcggc
      961 agacgtggac gagatactcg acgccacgcg gcgcctcggc gaaggcgaga ccctgctctc
     1021 ccaggatgag ctcgtcgcgt tgctccgcct cgccggacag aaccgcgaag aggaactcga
     1081 ggcgcgcgca agcatagagc agatcacacc ccgcgaaagg gaggtcctcc agacgctcgc
     1141 cgagggcctc tcgaacaagg agatcgccgc caaactgcat atgagcgtgg ataccgagcg
     1201 cacgcacatg atgaacatcc tgaacaagct cggcgtgcac tcgcggcttc aggccctgct
     1261 gttcgcggcc cgccacggca tcgtcgagat ccgctagagc ggtattcagg tagtgaacta
     1321 cctgaatacc gcggcttcag cagtcaggta tcagtagtca ggacttcttt ctcgcgactg
     1381 agcgacagct cgccttcgca gacgtactct gcgggtccct tcatgtatac gggctcgcct
     1441 tcgcctgacc actcgatctc aacaatgccg ccgtcgagtc gcacgctgac gggactctcg
     1501 gcgagaccca gcttcactgc cgcgaccgcg acggcgcacg aacctgaccc ggaagcaagg
     1561 gtctcgcccg ctccacgctc ccagatcctc attctgacct cgcgctcgcc ccgcgcgaac
     1621 ccgaactcca cgttcgtctt gtttgggaag acggggtcgt tctcgatcgg cgggccgacg
     1681 gcgcggagat ccagggtctc caccgccgcc tcgctctcca ggaaggtgac cgcgtgcggg
     1741 ttgcccatcg agacgcgcag gaagcggaag ccaccgacat cgatctctgt gtcgaatccg
     1801 ggagctccca tgtccacgcg cgaggagccg tcctcgaaga gaatgacctt cttgaccccg
     1861 gcgcccgtcg cgacggttat ggcttcgccc tcgaccataa cgcggtccct cgcgtagcgg
     1921 gccaggcagc gcaggccgtt gccgcacatc tccgagcgcg agccgtccga gttcaggtag
     1981 accatctcga tgtcggcggc ctctgaagga gaagggacca ggatcccgtc agcccctacc
     2041 ccgaagtgcc tgtcgcaggc cctcagagcg agttcgggca ggtcgacacc ttcgacctcg
     2101 cgggggtcga agatgagaaa atcattgccg cagccttgca tcttggtgaa gtgcatctct
     2161 cgctaacgcc ctctccagcc ctccaggtca actccagcgt gccgtcttcg tgcgcgacga
     2221 tccgaagccc aagggtagtg tacacgcccc ggtagcgctc ggccctctcc cgctcacgga
     2281 cgcgccggag ttcttccatc tcctccggcg tgcgctcgcg gaacatgttg ggcgagggcc
     2341 ggaacacggg caaacggccc tcggctgcgg cctcgcgcct gcactcctcg tgctcttctg
     2401 tgtaggtatg ctcgcggatg gcgtcgtccc tcccgtgcac gaggtcgggg agctcgcgca
     2461 ggtgctcgtc tataagggtt gcgtacgacc tcaggtcgtc gaggcgcctc tgaccgaccc
     2521 gcaaggcggc cagctcgtcc tccgcctcgg ccctgcgctc gtcaagctcg cccaggtgcg
     2581 ctcgtccgcg cgggaggggc gaacgttgtc ctttatcgcc gctcgggcag cgtccaccca
     2641 ctcggtcggc acgcccgcgt cggacacggg catcgctacc cattcctccc gactgttggg
     2701 cgtgatgtgg atggccttgt cgccgtccgg ggtccgctct cagcgcgtgc ggttgtacca
     2761 ggcgatcccg tagcgtgcgt cgcggtcgag cccggccagc acctcgggcg agaggttccc
     2821 cgtctcgacg aaccgctcga gttcgtcccg cccgtgcgcc cggtacacgt cgttgcagat
     2881 catctcgcgt atggttccgg cgtgccagta gcgtccgccc cccggcgtcg gcacgccgtc
     2941 cctctggaag gccgtcttta ccgcgcgcag ggtcgtgtac cctcgtttcc caccatgcgg
     3001 aaagcacgct tgacgtggcg cactcggcct tcgtccaccc gctcgccgat acagagcgga
     3061 taagatatta cgtgcatcaa gactcgcagg aactttgcgc gtctcagcta gtcgccttac
     3121 tcacggacct gccaccgctc tggatcttcg attgctatac aaaccgaaag ggtagctttc
     3181 cgttagattc tgttatcgta atcggcgttg accgacactg agtcggtcgc gacaacagcc
     3241 ctcgccggcg cagatccggc atgacgaaga ggggtgtgcc ccgagcttcg ctggtaatga
     3301 attctcggaa ctcagcctag ccctggtcct ataccgagcg atgttcttat gtacaaaagc
     3361 ccgcgatctc gtggcctgtc gtacataaga gttcaagtgt acccctcgga gggagctgcc
     3421 cttctggggt gtgcctggtc tgtagactgg ggctataagc aggctatccg gagagaacgg
     3481 gacgtgtacc atttgcggag ggtagatatt cggggaggta gcatgacggt tcgcggcttg
     3541 atggtgctgg cggtgttcgc agttctctat agctgtgggc aggcaagttc gcctgtcgag
     3601 aaccaggaga agaagcaagg agtcgtggag caggccagga gggaggtgcc ggaggcgcct
     3661 tcgatcgggt gcgcgctgtt ccccgacgac agctactggc acgccgacgt gtcgaacctc
     3721 cctgttcacc cggagtcgga ggcgttcatc gacgctatcg gcagggacac cccccttaag
     3781 gccgacttcg gtgccggcct gtacgaaggc tcctggatcg gcatcccgta cacgggcgtc
     3841 gatggcgacc agcccagggt ggacgtggcg ttccgctacg acgaggagag tgatcccgga
     3901 ccctacccaa tcccctcgga cgctcccctc gaaggcggcc ccgagtttgt caaggacaac
     3961 gaccgccacg tcctggtggt cgagcgagac tcttgcactc tgtacgagct gtataaggcc
     4021 gaatcgaagg gcgacggcac ctggaaggcg ggctccggag ccgtcttcga cctcgactcg
     4081 aacgacctgc ggcccgagac cttcacctca gccgacgcgg ctgggcttcc gatccttccc
     4141 ggcctggtgc gctacgatga ggtggcctcc ggcgagatcg accaccccat ccgcttcacc
     4201 ctcgaggaga cccgcgacca gtacgtctgg ccggcccgac acgaggctgg ccagcccggc
     4261 gctgacctgc cccccatggg cctgtggctg cgcctcaagg ccgacttcga cacctccggt
     4321 ttccccgaag agctccaggt gattctacgg gccatgcaaa agcgcggcct gatcctggcc
     4381 gacaacggct cctcgatgtt catctccggg gtgcccgaca accggtggga taacgacacg
     4441 ctcgctcgcc tgcgtgagat cgacgcaggc gacttcgagg cggtcgacgt ctcctcccta
     4501 aaagt
//
LOCUS       DATAIR010000079         6007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_281700, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000079 DATAIR010000000
VERSION     DATAIR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281700"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VHM69_02055"
     CDS             <1..1239
                     /locus_tag="VHM69_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2739203.1"
                     /translation="LAFGLCGTFWSQAVIPEVYTLEALLVALVVLVLLVWRDRRDDRY
                     LLLTAFLVGLSLTHHLTSVLLVPAVPAFVLLTDRRVSSRTRLLLKGLGLFLLGLLPLL
                     YLPVRAMMHAPLNEADPSTPWRLLMLVTGGSFLAESSEKGRQCSPSNLALADPSTKLQ
                     ILGDHLLGQFPLVLMLVGVLAAVYLLFTDRAIAALFGTLFLGCLLQAIVYLQLGIEDY
                     YVFLIPAYLVFALCISVGLAALFRLVDSLLVSPAIRKVLIFAISALMLVVPLLGLREA
                     YAARDRSGDYEGRKTIEAVASNVKEDATVLHHRSPLWYMVLVERRRRDITLIDPFCTS
                     WDRHTDIVWPDSINANEAAARYGTDDTTGVDAARRAAKKGPVYLLAHDRARLTLFREA
                     DFDVVRKDSLLYELVLQRPQ"
     gene            complement(1275..1940)
                     /locus_tag="VHM69_02060"
     CDS             complement(1275..1940)
                     /locus_tag="VHM69_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739204.1"
                     /translation="MDRIRILIADDHPFFRDGLRVLMDATPDTELVGEATDGDEAVTL
                     AAKLKPDVVLMDLRMPGLGGIEATRKVLEEIPETGILVVTMVEDDDSVFAAMRAGARG
                     YLLKGADRDEMLLAIRAVARGEAVFGPGIARRLIQYFSAPRQGPSRKPRSIFPELTDR
                     EREILDLVAAGRNNQEIAESLFLSLKTVRNYVSNIFTKLHVADRAQAVVRAREAGLGR
                     KDG"
     gene            complement(1943..4114)
                     /locus_tag="VHM69_02065"
     CDS             complement(1943..4114)
                     /locus_tag="VHM69_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2739205.1"
                     /translation="MNHSLAPSAASSDRTYDAPLRGRPLLVARTAWVSLAVLTVGLFA
                     AGIPAELALLRISCPTSICTTGQLSPAGLRALRDLDLSPGSFAAYTVTMDVLFAAVCG
                     SVAGLIFWRKSDDRMGLFASLALLTFGTATFTFTLAALAARHSAWEIPVSFLHFLGAA
                     SFGLFLYLFPDGRFVPRWTRWVALVWIAWQLAEHFFPNWSSHPNAWQVGTETVVWLGA
                     LGAAIYSQIHRYRRASNSVQRQQIKWVVFGISAALAVFLGLQLALGVSGADAPTSAGA
                     LVMYMIWYTLLSYLAVLLIPVSIGIAVLRHHLFDIDVIINRTLVYGALTTSVVGIYVL
                     VVGGLGTLLQARGNFLVSLLAAGLVAVLFAPLRIRLQRSVNHLLYGERDDPYAVLSRL
                     GQRLESTLVPDAVLPTVVRTVAEALNLPYVAVELERDGAYEIAATAGDSAGDPVRLPL
                     VYGGETVGRLVLEPRAGDESFSTSDRRLLEDLAHQISVAAHAVRITDEAVRLSADLQR
                     SRERLVKTREEERRRLSRDLHDGLGPTLGSLPMKLDVAGDLLERDPTATRTLLHGLKW
                     QAQSAVADIRRLVYELRPPALDELGLVGAIRETAAQYAQNGLRVSVSVKEELSSLPAA
                     VEVAAYRIVQEALTNVAHHAAAGECSVTLKLDAGTLDLEIQDDGRGLSPERGQGVGLY
                     SMRERAEELGGSCIVESPPGAGARVRATLPCTTPARSRSGE"
     gene            complement(4273..4974)
                     /locus_tag="VHM69_02070"
     CDS             complement(4273..4974)
                     /locus_tag="VHM69_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2739206.1"
                     /translation="MPEQMWERAGFAIGVAGSAVIAGAVIVSRFRSRPGKMRRKTGHE
                     SSRPLVGHEFKLMGHTFRVLESARDTDDGSLRLDYSAPPDANVSEHVHRFQEERFEVL
                     SGKLGVRVGGQEMILCPGQRGVGPPGVPHAWWNPSGDERVRFLVAVLPGIEVETMFET
                     LLGLMREGRTIGHLPRNPLQLAVLAREIANWLVLTPGQKTLFAPVVGLAIVGGLFGYR
                     ARYPEYSDPDGTGNE"
     gene            complement(4985..5611)
                     /locus_tag="VHM69_02075"
     CDS             complement(4985..5611)
                     /locus_tag="VHM69_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX2739207.1"
                     /translation="MSNRIDLLAILAIVGWTFFAASALFLPIVSEYSFTGDYISELAI
                     GRFGYVQTVAFFAAGLGSLALALGIRETTRGSWGSRAGFAMVGLFGINVFLAGIFPTD
                     AIDAEGRVLSPTAVGAIHIVVSALAFVLIIAAMFVLSRTFKQDIRWRSFWPVSLVLAF
                     AAFVTFFLTSDGQWTGLYQRVFIGTIVLWLILAAIRLRSIARMSAPTG"
     gene            complement(5623..>6007)
                     /locus_tag="VHM69_02080"
     CDS             complement(5623..>6007)
                     /locus_tag="VHM69_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739208.1"
                     /translation="VAPVLAARYGTPGGLDPQLARSLLDIEEASYFIAFLPLAVLLAA
                     FAIIAIRTGDLPGWLGWLAAALSAAFLSGGLVGSANLASEWAQVPMLVYPVWVIATSV
                     VLIRRAGEASPVETATPTGRAAPVG"
BASE COUNT         1111 a         1961 c         1891 g         1044 t
ORIGIN      
        1 ctggcgttcg ggctctgtgg caccttctgg agccaggccg ttatccccga ggtatatacg
       61 ctcgaagccc tcctcgttgc gctcgtcgtc ctggtcttgc tcgtctggag agaccgccgc
      121 gacgaccgct acctgctcct gaccgcgttc ctcgtcggcc tatcccttac ccaccacctg
      181 acgagcgtcc tgctcgtccc cgccgttccg gccttcgtgc tcctgaccga tcggcgcgtg
      241 tcttcgagga ccagactgct gctgaagggc ctcggcctct tcctgctcgg gctacttccc
      301 ctcctttacc tcccggtacg ggccatgatg cacgctcccc tgaacgaggc tgatccctcg
      361 acaccctggc gtctcctgat gctcgttacg ggcggcagtt tcctggccga gtcatcggag
      421 aaagggaggc aatgcagccc atcgaacctc gcgctcgcag atccttccac caagcttcag
      481 attctcgggg atcacctcct cgggcagttt cccttggtat tgatgctggt cggagtactg
      541 gcggccgtct atctactctt tacggaccgg gcaattgccg ccctcttcgg tacgctcttc
      601 ctcgggtgcc tgctccaggc catcgtgtac ctccagctgg ggatcgagga ctactacgtg
      661 ttcttgatcc ccgcctatct ggtcttcgcc ctgtgtattt cggtaggcct cgcagctttg
      721 ttccggctgg tggatagcct attggtctca cctgctatca gaaaggtctt gatcttcgcc
      781 atatctgcat tgatgctcgt cgtaccgctg ctgggactgc gcgaagccta cgccgcgcgc
      841 gaccgtagcg gggactacga aggtcgcaag acgatcgagg ccgtggcgag caatgtgaaa
      901 gaggatgcca cggtgctgca ccacaggagc cctctctggt acatggtgct tgtggagcga
      961 cgcaggagag acatcaccct catcgacccc ttctgcacct catgggaccg ccacaccgat
     1021 atcgtctggc ccgactccat caacgcgaac gaggccgcag cacgctacgg caccgacgac
     1081 accacaggcg tcgatgccgc gcgcagggcc gcgaagaaag ggcccgtcta ccttctcgcc
     1141 cacgaccgtg ctcgattgac gcttttcagg gaggcggact tcgacgtggt ccggaaagac
     1201 agcctcctgt acgaactggt gctccaacgg cctcaatgag gcggtggccg ggcgaataga
     1261 ggatcggtgc gtccctatcc gtccttccgg ccgagccctg cttcacgggc gcgcacgact
     1321 gcctgggcgc ggtcggcgac gtgcagcttg gtgaagatgt tggagacgta gttgcgtacg
     1381 gtcttcaggc tgaggaagag gctttcggcg atctcctggt tgttgcggcc cgcggcgacg
     1441 aggtccagga tctcacgctc ccggtcggtg agctcgggga agatggagcg gggcttcctg
     1501 gacggtccct gtcggggagc gctgaagtat tggatcaggc gccgcgcgat gccgggaccg
     1561 aagacggcct cgccgcgcgc cacggcccgg atcgcgagca acatctcatc cctgtccgct
     1621 cccttgagca ggtagccgcg agccccggcg cgcatggcgg cgaagacgga gtcgtcgtcc
     1681 tcgaccatcg tgacgacgag gatgcctgtc tccgggatct cctccagaac ctttcgggta
     1741 gcctcgatgc cgcccaggcc gggcatacgg aggtccatca ggaccacgtc aggtttcaac
     1801 ttcgccgcca gcgtaacggc ttcgtcgccg tcggtcgcct cgccgacaag ttccgtgtcg
     1861 ggtgtagcat ccataagaac gcgcagcccg tcgcgaaaga acggatgatc gtcggcgatc
     1921 aggattcgga tgcgatccat gctcactccc cggacctgga tcgtgcgggt gtcgtgcatg
     1981 gcaaggtggc ccgcacgcgc gctccggccc cgggcggcga ctctacgatg cagctaccgc
     2041 ccaactcctc tgcccgctcg cgcatcgagt agagtccgac gccctgacca cgttcagggg
     2101 agagcccgcg cccgtcgtcc tgtatctcca ggtcgagcgt ccccgcgtcc agtttgaggg
     2161 tgacggaaca ttctccggct gcggcgtggt gggcgacgtt ggtcagggcc tcctggacga
     2221 tccggtaggc tgcgacctcg acggcggcgg gcagcgatga tagctcctct ttgacactaa
     2281 ccgagacgcg cagcccattc tgggcgtact gcgctgcggt ctcgcgtatg gcgccgacca
     2341 gtccgagctc gtcgagtgct ggaggacgca gctcgtacac cagccggcgg atatccgcga
     2401 cggcgctctg ggcctgccat ttgagcccgt gcaacagggt acgcgtggca gtggggtctc
     2461 gctccagcag gtcgcctgcc acgtccaact tcataggcag gctacctaga gtcgggccga
     2521 gaccgtcgtg caggtcccgg ctcaggcgcc ggcgctcctc ttcccgcgtc ttgaccagcc
     2581 gttccctgga acgttgcaag tcggcggaga gccggacggc ttcgtcggtg atgcgcacgg
     2641 cgtgggcggc gacgctgatc tggtgcgcaa ggtcttccaa caagcgtcgg tctgatgttg
     2701 agaacgattc gtcgccggcg cggggttcca ggaccagacg gccaaccgtt tcgcccccgt
     2761 agacgagcgg aagcctgacc ggatctccgg cagagtcacc agccgtcgcc gcgatttcgt
     2821 acgcgccgtc tcgctcgagt tcgacggcaa cgtacggcag gttcaacgcc tccgcgaccg
     2881 tcctgacgac ggtgggtagt acggcgtcgg ggacgagcgt ggattcgagg cgctgtccga
     2941 gacgcgagag caccgcgtaa gggtcgtcgc gctccccgta cagcagatga tttacgctgc
     3001 gttgcaggcg aatgcgcaac ggcgcgaaca ggaccgcgac gagtcccgcg gcgagcaggg
     3061 agaccaggaa gttcccacgt gcctgcagta acgtccccag tcctcccaca acgagaacgt
     3121 agatcccgac gacgctcgtg gtcagcgcgc cgtagacgag cgtgcggttg atgatgacgt
     3181 cgatgtcgaa gagatgatga cgcagcacgg cgatgcctat ggataccggg atgaggagca
     3241 cggcgaggta actgaggagc gtgtaccaga tcatgtacat gaccaacgcg cccgccgacg
     3301 ttggcgcatc cgccccggag acgcccagcg caagctgcaa accgagaaat accgcgagcg
     3361 ccgccgagat tccgaagacg acccatttga tctgctgccg ctgtactgag ttcgaggcac
     3421 gccggtagcg atggatctgg gagtagatcg cggcgccaag agcacccaac cacaccactg
     3481 tctccgtccc gacttgccag gcattcgggt gagaggacca attggggaag aagtgctcgg
     3541 ctagttgcca cgctatccac accagcgcca cccagcgcgt ccagcgaggc acgaaccgtc
     3601 catccggaaa caggtacaga aacaggccga aggaggccgc gccaaggaag tggaggaagg
     3661 agaccggtat ctcccatgcg gagtgccgtg ctgccagcgc cgccagggtg aaagtgaagg
     3721 tcgccgtccc gaacgtcagg agcgcgagtg aggcgaacaa ccccatacgg tcgtccgact
     3781 tgcgccagaa tatgaggccc gcgaccgagc cgcagacggc agcaaacagg acgtccatcg
     3841 tgaccgtgta ggccgcgaag gatccgggcg agagatccag gtcccgaagc gcgcgcagac
     3901 cggccggaga gagctgccct gtggtgcaga tagaggtcgg gcacgatata cgcagcagcg
     3961 ccaactcggc ggggatgccg gcggcgaaca ggccgacggt tagcacagct aaggacaccc
     4021 atgccgtgcg tgcgactagc agaggtcggc cgcgcagagg cgcatcgtac gtccggtcgg
     4081 agcttgcggc ggatggcgcg agggaatggt tcacgtacaa actccccgaa tacgcgcccg
     4141 atgcccggtc cccggaaccg gccaggatct cacttccgtg ctcctctctt tcactcgaca
     4201 ttctatctcg gaagatgacc ttccggatcg ggattcttac tctttgcttg cctgagcaca
     4261 gaacccatct gattactcgt ttcccgtgcc gtcaggatca ctgtactcgg ggtagcgagc
     4321 cctgtacccg aacagcccgc cgacgatcgc gagacccact accggcgcga agagagtttt
     4381 ctgccccggc gtcagcacca gccagttggc gatctcacgc gccagcacag cgagctgaag
     4441 cgggttcctg gggagatgcc cgatggttcg cccttcgcgc atgaggccga gcagcgtctc
     4501 gaacatggtc tccacctcga tccccggaag gacggcgacc aggaatcgta ccctctcatc
     4561 gccgctgggg ttccaccatg cgtgaggaac accaggcggg ccgaccccgc gctggcccgg
     4621 acaaaggatc atctcctgac ctcccacgcg gacgcccagt ttcccggaga ggacctcgaa
     4681 acgctcctcc tggaagcgat ggacgtgctc tgagacgttc gcatccggcg gggcggagta
     4741 gtccaaccgc agcgatccgt cgtccgtgtc gcgggccgat tctaggacac ggaacgtgtg
     4801 tcccatgagc ttgaactcgt gaccgacgag tggtctgcta ctctcgtggc ccgtcttgcg
     4861 acgcatcttc cctggcctgg aacggaagcg cgacactatg acggctccgg caatgactgc
     4921 cgagccggcg acacctatgg cgaacccagc tcgctcccac atttgttcag gcatggctat
     4981 ctcctcaccc tgttggcgcg ctcatcctgg cgatggaacg cagccggatc gcggcgagga
     5041 tcagccacag cactatcgtc ccgatgaaga cccgctggta taacccggtc cattggccgt
     5101 cactcgtcag gaagaaggtt acgaacgccg cgaacgccag cactagcgag acgggccaga
     5161 acgatctcca cctgatgtcc tgtttgaacg tcctcgaaag tacgaacatc gccgcgataa
     5221 tcagtacgaa ggccagcgcg gagaccacga tgtgtatggc ccccacggcc gtcggcgaga
     5281 gcacccggcc ttccgcgtcg atggcgtctg tagggaagat ccccgcgagg aacacgttta
     5341 tgccgaagag gcccaccatg gcgaaaccag cgcgcgagcc ccaggagccc cgcgtcgtct
     5401 cgcggatgcc cagggccagt gccagagaac cgagcccggc ggcgaagaac gcgaccgtct
     5461 ggacgtagcc gaacctgccg atggcgagct cgctgatgta gtccccggtg aagctgtatt
     5521 ccgagactat cggcaagaat aaggcggatg ccgcgaagaa cgtccagccg acgatcgcga
     5581 gaatggccag aaggtcaatc cgattggaca cgtctatctc cttcacccca caggcgccgc
     5641 ccttccggtt ggcgttgccg tctcgaccgg gcttgcctct ccggcgcgac ggatcaggac
     5701 tacgctcgtt gcaatgaccc agaccgggta cacaagcatc ggcacttgcg cccactcgga
     5761 cgcaaggttg gcgcttccga ccagcccacc ggagagaaac gctgcggata gtgccgcggc
     5821 cagccaacca agccaccctg gcagatcgcc tgtccggatc gcaattatgg cgaaggcggc
     5881 cagcaggacc gccagtggga ggaaggcgat gaagtaggaa gcttcctcta tgtccagaag
     5941 cgacctcgcg agttgcgggt cgagccctcc gggagtcccg tagcgggccg cgagcacggg
     6001 tgcgacg
//
LOCUS       DATAIR010000080         7709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_4880, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000080 DATAIR010000000
VERSION     DATAIR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1143)
                     /locus_tag="VHM69_02085"
     CDS             complement(46..1143)
                     /locus_tag="VHM69_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739209.1"
                     /translation="MSDFVTRLAERAMGTAPVVQPLIASMFAPEPDGGLMEPEDETAT
                     VPEVQDLTPRAQTPPETPMPVPDEAAPQNEEPDTGVTPLSNAPGAAPSARPAPSKQTP
                     ASGQEDLDATDTPGPPMRPRTGEPGPLETGMESMQEDRQTSSSIPPTPIAPGPGSGTS
                     FPEAPDPSGRQVLPGIRPRRESLTTTADGRNPPPAPRFESAPRVESAPERRDVEETSR
                     RLVPKDSPSSSYAADYGRQRAMLSPTTELAEPVRVTDEAPAPPPGAGDPLETSEVTKV
                     EIVQAHRAVQGAATVVVPRIVRHQPDQREESGPREASVLAPEPPAPTIKVAIGRIEVR
                     SVTPPAPPRRETPARPPLSLDDYLAQRNGGR"
     gene            complement(1143..1871)
                     /locus_tag="VHM69_02090"
     CDS             complement(1143..1871)
                     /locus_tag="VHM69_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739210.1"
                     /translation="MRPLSAHDLLRVWELGEDQHPLDRALTILATACPELTWDELATL
                     SIGQRDARLLALREHTYGPKLEGFVECPTCGASLEFDVMAADLRATETAAEQDLALAS
                     DGFELRFRLPDSRDLAAVLGCRDLTEARDLLMQRCVLEARREGTPVADSELPADIAAE
                     VSRSMSEHDPQAEVLIDLRCPTCHYRWRALFDIVTFFWTELSAQAKRLLREVHALARA
                     YGWREADILGMSARRRQFYLELAT"
     gene            complement(1876..2412)
                     /locus_tag="VHM69_02095"
     CDS             complement(1876..2412)
                     /locus_tag="VHM69_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX2739211.1"
                     /translation="MAQFSVNVDPQRFDPYKNFKFRVRFGDNPDYVAGVSKISALRRT
                     TEVVSHREGGDPSSDRKSPGRTQYEAITLERGVTHDTEFEKWANKVWNFGSGLGAEVS
                     LKDFRKDIIIDFYNEAGQLAIAYKVFRCWVSEYQALPELDANANAVAIQTMKLENEGW
                     ERDYAVTEPTEPSFTEPG"
     gene            complement(2423..4180)
                     /locus_tag="VHM69_02100"
     CDS             complement(2423..4180)
                     /locus_tag="VHM69_02100"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX2739212.1"
                     /translation="MPITPTYPGVYIEEIPSGVRTITGVATSITALVGRTRRGPDNEP
                     FTINSFADFERTFGGLWEHSTVAYALRDFYRNGGSQAIVVRLFHPSFDSDEERQDAYE
                     EALEDAQTAAGNVAEAARTAAGEAGADADSVADAAEEAAGTPADPGPAATEAAEAAAP
                     VTRAQLSVDTLDLEARYPGTWGNELRARVDDDVADPGSGLFNLSILDGETGDIELFRN
                     VSVDASHPRRVDKVLEAESKLVRALGELPPDPPESNDDVEPGGNPFSDDTSIGVEDSG
                     VPASDGKDLVAADFTGAGMEAGKLGLFALEKADLFNILCIPPYSRTEDIGSDLWADAA
                     AYCERRRAMLLVDAPGDWESAADARDNLGTDIGLTSKNGAIFFPRLRQPDPARDNRPE
                     DFAPCGAVAGVFARTDTERGVWKAPAGLGATLVGVPELTVPLTDAENGMINPLGINAL
                     RTLPAAGRVVWGSRTLQGDDRLASEWKYVPVRRLALYIEESLYRGTQWVVFEPNDEPL
                     WAQIRLNIGAFMQNLFRQGAFQGKSPQEAYFVKADSESTTQNDINLGVVNIIVGFAPL
                     KPAEFVIIKIQQMAGQIAV"
     gene            4667..4798
                     /locus_tag="VHM69_02105"
     CDS             4667..4798
                     /locus_tag="VHM69_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739213.1"
                     /translation="MAKERRPYIVGVKKIAREARDTFNSYVPDRESMYEDIDGGGRG"
     gene            4809..5036
                     /locus_tag="VHM69_02110"
     CDS             4809..5036
                     /locus_tag="VHM69_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2739214.1"
                     /translation="MAVGGGETTMSGTSMATPIVARVIACLLATGRPVAEVRQALLAG
                     TVDTNYPVTSEGRGVVKALKALGKLPLTQAA"
     gene            5204..6541
                     /locus_tag="VHM69_02115"
     CDS             5204..6541
                     /locus_tag="VHM69_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEX2739215.1"
                     /translation="MSIAAPVFGIDVHPEYQKGFDFKRAKAQGYEYAFIKASEGPYRD
                     GTTYVPSGFKDFHDRAAASGLIVGLYHFLLESDGDDPQRGGRVQADLFHRTIETVGGT
                     QGKLIAVDFEAYSDKFPWLWPTNATLKAFISALRTRIGNHPIILYSARSYWNEGTPSG
                     DFDQYGAAVAWDARYADMKPHDAPKDNYKEVQDWGWGEPWGGVTPAFWQFTSSGLVAG
                     QYIDVDAYRLTLKDLRSLAVTPLPHPDEPEEEEPETPPSSDDGVMNNVQSVKQYGLAL
                     LEQQPKYWCWDQGNLNIPRPAVGRPACVDGPPPKLANIKRIFCADLISLQLRHLGKPV
                     PKNHGSFGNFDGGTRSFRLRYGNQMKPFKLSECREGDVAFVDFQTSWAPEGHIGFCLG
                     DGPDAKFLQSHLETPCVTGEPGLNNTYTLGQSNTRTGGGNYYTQRIPREVIWG"
     gene            6768..6977
                     /locus_tag="VHM69_02120"
     CDS             6768..6977
                     /locus_tag="VHM69_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739216.1"
                     /translation="MNDATQQRIITILAAAIAYLISQYVTDRLIDIPDERGIKDDVLE
                     AVLKGATTATSTILASVLVRRLFRG"
     gene            7087..>7709
                     /locus_tag="VHM69_02125"
     CDS             7087..>7709
                     /locus_tag="VHM69_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2739217.1"
                     /translation="MAGPLEGRVAIVTGGGGGLAEGVCARLATDGAAVAAVDISQEKA
                     ERVAGQLRAEGASAGAFEADVSDRRSVEAMVGEVTREFGGVDILVNNAAVYPRRAWTE
                     IEEEEWDRVMAVNLKGYFLCARAVYPYMLERGRGNIVNVASITFFIGWELLLDYVASK
                     GGIVGFTRTLAREVGPDNINVNAIAPGAFPTDAEKIHPDQEGYNRWVL"
BASE COUNT         1336 a         2414 c         2562 g         1397 t
ORIGIN      
        1 tcttcagcag cgcggttacg gcgccgattg ccaggaaatt gctcatcacc gcccaccgtt
       61 acgctgcgcg aggtaatcgt cgagggagag cggcggcctt gccggcgtct ctcgccgcgg
      121 gggcgccggt ggcgtgacgg aacgcacctc gatacggcct atcgccacct tgatggttgg
      181 cgcgggcggc tccggcgcga ggacgctcgc ttcgcgtgga ccgctttctt cccgctggtc
      241 aggctgatgg cgaacgatcc tcggcacgac caccgtggca gcgccctgca cggcgcgatg
      301 ggcctggacg atctcgacct tcgtcacttc gctggtttcg aggggatcgc cggcgcccgg
      361 cggaggcgcg ggcgcctcgt ccgtgacccg gacgggttcg gcgagctccg tcgtcggact
      421 cagcatggca cgctgccgtc cataatccgc cgcgtacgag ctggatggcg agtctttggg
      481 caccaggcgt cgtgaggtct cctcgacatc gcgccgctcc ggtgcggatt ctacgcgggg
      541 tgccgattcg aaccggggcg caggcggcgg gtttcggcca tctgcggtgg tggtcaggga
      601 ttccctgcgg ggccttatcc caggaaggac ctggcgcccc gacggatcag gtgcctctgg
      661 gaatgacgtt ccggatccgg gccccggtgc gatgggcgtg ggcggaatcg aggaggaggt
      721 ctgtctgtct tcctgcatgg attccatccc ggtctccagc gggccgggct cgcctgtgcg
      781 cggtctcata ggaggacccg gtgtgtcggt agcgtccagg tcctcctgcc cagaagcagg
      841 ggtctgcttt gaaggtgcag ggcgggcgga aggtgctgca cccggagcgt tggacagagg
      901 cgttacgccg gtgtccggct cctcgttttg aggcgccgct tcatcaggca ccggcatggg
      961 tgtttcgggt ggtgtctggg cgcgcggcgt cagatcctgg acctccggca cagttgccgt
     1021 ctcatcctca ggctccatca ggccgccgtc gggctccggg gcgaacatcg aagcgatcag
     1081 cggttgcacg acgggtgccg tgcccatagc tctttcggcc agcctggtca cgaaatcgct
     1141 catcaggtag ccaactccag gtagaactgg cgtcgtcggg cgctcatccc caggatgtcc
     1201 gcctcgcgcc agccgtaggc ccgcgccagg gcgtggacct cgcgcaacag tcgcttggcc
     1261 tgggcgctga gttcggtcca gaagaaggtc acgatgtcga agagcgcccg ccagcggtag
     1321 tggcacgtcg ggcaacggag gtctatcagc acctccgcct gcgggtcgtg ttctgacatg
     1381 gatcgggaga cctcggcggc gatgtctgct ggcaactcac tgtccgctac gggggtaccc
     1441 tcgcggcggg cctccagcac gcagcgttgc atcagcaggt cgcgtgcctc ggtgaggtcc
     1501 cggcacccga ggacggcggc cagatccctg ctgtctggca gccgaaaacg tagctcgaag
     1561 ccgtcgctgg ctaacgcaag gtcctgttct gctgcggtct ctgtcgcacg gaggtccgcc
     1621 gccatcacgt cgaactcgag gcttgcccca cacgtcgggc actctacgaa cccttccagc
     1681 ttcggtccgt acgtgtgctc gcgcagggcg agcaggcgcg cgtcccgctg gccgatgctt
     1741 agcgtggcca gctcgtccca ggttagctcc gggcaggccg tggcgaggat cgtcagcgcc
     1801 ctatcgaggg gatgctggtc ctctcctagt tcccaaaccc ggaggaggtc gtgggcggac
     1861 agtgggcgca tcgctctagc cgggctctgt gaagcttggc tcggtaggtt cggtaaccgc
     1921 atagtcgcgc tcccagcctt cgttctcgag cttcatggtc tggatggcga ccgcgttggc
     1981 gttggcgtcg agctcgggca gggcctggta ctccgagacc cagcagcgaa ataccttgta
     2041 ggcgatggca agctggccgg cctcgttata gaagtctatg atgatgtcct tgcggaaatc
     2101 cttgagcgac acctcggcgc caagcccgga gccgaagttc cataccttgt tcgcccactt
     2161 ctcgaactct gtgtcgtgtg tcacgccccg ctcgagcgtg atggcctcgt actgggtacg
     2221 tcctggcgac ttgcggtcgc tgctcgggtc tccgccctcg cggtgcgaga ccacctccgt
     2281 tgtccgcctc agggcactga tcttgctcac cccggcaacg tagtccgggt tgtccccgaa
     2341 cctgacccgg aacttgaagt tcttgtatgg gtcgaaccgc tggggatcga cgttgacgct
     2401 gaactgggcc atctcttcct ccttacaccg cgatctgtcc ggccatctgc tggatcttga
     2461 tgatcacgaa ctcggccggc ttgagggggg cgaacccgac gatgatgttg actacaccga
     2521 ggttgatgtc gttctgggtc gtgctctctg agtccgcctt gacgaagtag gcctcttggg
     2581 gagacttgcc ctggaacgcg ccctggcgga agaggttctg catgaaggcg ccgatgttca
     2641 gacggatctg cgcccacagc ggctcgtcgt tgggctcgaa gacgacccac tgtgtccccc
     2701 tgtagaggct ctcctcgatg tagagggcca gcctcctgac gggcacgtac ttccactccg
     2761 aggcgaggcg gtcgtcgccc tgcagcgttc gcgagcccca gaccacgcgt ccggcggcgg
     2821 gcagcgtacg caaagcgttg atgccgaggg ggttgatcat gccgttctcg gcgtccgtca
     2881 gcggcaccgt gagctccggt actccgacga gggtggcccc cagcccggca ggcgccttcc
     2941 agacgccgcg ctcggtgtcg gtgcgggcaa atacccccgc caccgcgccg cagggggcga
     3001 agtcctccgg cctgttgtcc cgcgccgggt cgggctggcg tagacgcggg aagaagatcg
     3061 ccccgttctt gctggtgagc ccgatgtcgg tcccgaggtt gtccctggcg tcggccgcgc
     3121 tctcccagtc cccgggcgcg tcgaccagga gcatggccct gcgccgctcg cagtaggcgg
     3181 ccgcgtccgc ccacaggtcg gacccgatgt cttccgtccg cgagtatggg gggatgcaca
     3241 ggatgttgaa gaggtctgct ttctccagag cgaacagccc gagtttgcct gcttccatgc
     3301 ccgcgcccgt gaagtcggct gcgacgagat ccttgccgtc agaggcaggt acgccggagt
     3361 cctcgacgcc gatcgaggtg tcgtcggaga acgggttccc acccggctct acgtcgtcgt
     3421 tggattcggg tgggtcgggg ggcagttcgc cgagggcacg gacgagcttc gattcagctt
     3481 ccagaacctt gtctaccctg cgcggatggc tcgcatcgac cgacacgttg cggaagagct
     3541 cgatgtcgcc ggtctcaccg tcgagtatcg aaaggttgaa aaggcctgag ccagggtccg
     3601 ccacgtcgtc gtcgaccctc gcccgcagct cgttgcccca ggtgccagga taccgtgctt
     3661 ccaggtccag cgtatccacg ctgagctgtg ccctggtgac gggcgccgcc gcttccgcgg
     3721 cctccgtggc agctggaccc gggtccgcgg gtgtgccggc cgcctcctcg gcggcatctg
     3781 ccacgctgtc ggcgtcggcg ccggcctctc ccgcggccgt ccttgccgcc tcggccacgt
     3841 tgcctgccgc tgtctgcgcg tcctccaacg cttcttcgta cgcgtcctgt cgttcctcat
     3901 ctgaatcgaa agaagggtgg aagaggcgaa cgacgatggc ctggctgcct ccgtttctat
     3961 agaagtcccg aagcgcataa gccaccgtgc tgtgttccca gagaccgccg aaggtgcgct
     4021 cgaagtcggc gaagctgttg atggtgaagg gctcgttgtc ggggcctcgc ctggtgcggc
     4081 cgacgagggc cgtgatcgag gtggcgacgc ccgtaatggt gcgcacgccg ctcggtattt
     4141 cctcaatgta tacgccgggg tacgtgggtg tgatcggcat taactgttct ccttgtaagt
     4201 ctcaagtctg ccaggtgtcg gcgtatgggc tgtcatccca atggttacct cctcgtcggc
     4261 gagccgttcg cagcccgcac ttcttgcctg ctcgttcgtt gtgcatcggg cgagccgttt
     4321 accaggagat aaaagcaggt gacgatgaat cgcggatgaa tcgcggatga atcgcggatg
     4381 agacgaacga caaaggcgcc gaccgatgaa gggcgtcgta ctgaggccgg aaccaacgcg
     4441 gaggtctgca tcggaccgga cacaggcgct tgctctcgaa aattcatccg tgattcatcg
     4501 ccttctggtg ttattagggg ctggagcctc gaccgaaagg agcggcctat cggttaaaca
     4561 tcgggtgtaa acggataggt gtcctccgtg cgggctgcat caagaaacct gacgagcgcc
     4621 gggcgcatgc aatcccgatc caaccgggag gataaaagga gtatagatgg caaaagaacg
     4681 cagaccctac atcgtgggcg tgaagaagat agcgcgcgaa gcccgcgaca cgttcaacag
     4741 ctacgtccct gaccgggaga gcatgtacga ggacatcgac ggcggcggcc ggggttgatc
     4801 tggccggcct ggcagtcggc ggcggtgaga ctactatgag cggaacgtcg atggctaccc
     4861 ccatagtggc cagggtcatc gcctgcttgc tcgcgacagg gcgccctgtc gcggaggtgc
     4921 gccaggccct cctggccgga accgtcgata caaactaccc ggtgacgagc gagggacgag
     4981 gtgtggtgaa ggctctgaag gccctcggca aactgccgct cacccaggct gcgtgagaag
     5041 acgaccactc ctcaatgcca gcagcgcagg agcaaacgac gtatgaccga ggcgcgggaa
     5101 cacagcctga gcacgagctt ctctggcgag gggtgccacc acacggatta cgacaacgcc
     5161 aacagcaacc gcgtctggac ggcgcacgac ctttaggagg agtatgagca ttgctgcccc
     5221 cgtattcggg atagacgtcc accctgagta ccagaaaggc ttcgacttca agcgcgcgaa
     5281 ggcgcagggc tacgagtacg cctttatcaa ggcttctgaa ggcccctacc gggacgggac
     5341 gacctacgtc ccgagcggct tcaaggactt ccacgacagg gccgcggcgt ctggcctcat
     5401 cgtcggcctc taccacttcc tgctggagtc cgacggggac gacccgcagc gcggcggcag
     5461 agtccaggcc gatctcttcc atcgcaccat agagaccgtc ggcggcactc agggcaaact
     5521 catagccgtg gacttcgagg cgtactcgga caaattcccc tggctttggc ccaccaacgc
     5581 gacgctaaag gcgttcatct cggccttgag aacgcgcata ggcaaccacc cgatcatcct
     5641 ctacagcgcg cgcagctact ggaatgaggg cacgccctca ggggacttcg atcagtacgg
     5701 cgccgctgtg gcatgggatg caaggtacgc cgatatgaaa ccgcacgatg cgcctaagga
     5761 caactacaaa gaggtacaag actggggttg gggcgagccc tggggcggcg tgacaccggc
     5821 gttctggcag ttcacctcct ccggcctcgt tgccggccag tatatcgacg tggacgccta
     5881 caggctcacg ctcaaagacc ttcgctcgct cgccgtcacc ccgctgccac accccgacga
     5941 gccggaggaa gaggagccag agacgccgcc cagctccgac gacggagtga tgaacaacgt
     6001 tcagagcgtc aagcagtacg ggctcgccct ccttgagcag cagcccaagt actggtgctg
     6061 ggaccagggg aacctcaaca tccctcgccc cgcagtgggc agaccagcct gcgtggacgg
     6121 tccacctccc aagttggcga acatcaagag gatcttctgc gctgacctga tctcactcca
     6181 gctgcggcac ctgggtaagc cggtcccgaa gaaccacggc tccttcggca acttcgacgg
     6241 aggtacccgc tccttccgcc tcaggtacgg caatcagatg aagcccttca agctctcaga
     6301 gtgcagggag ggcgacgtgg cctttgtaga cttccagacc tcgtgggcgc cagagggcca
     6361 catcggattc tgcctcggcg acggtcctga tgccaaattc ctgcagagcc acctggaaac
     6421 gccctgcgtg accggggagc caggcctgaa caatacgtac accctgggcc agagcaacac
     6481 gcgtaccgga ggaggtaatt actacacgca acgcatcccg cgcgaggtca tctggggcta
     6541 gtacaaaagc aaacccggca agcgcgagga gttcttgcaa gggttccata tgtgcgcgtt
     6601 cgaaggcacc ctcgaatagg cagccgacag gatagtccac gccgaggaaa atccgggtgc
     6661 gtcggggcgt tgatcgaagc cgccgccttg cgcttcgtcg tgggttttcg cattgcctgg
     6721 cgtgggtatc gattcaagcg gaatgtaaga agaccttagg aaggcgtatg aacgacgcga
     6781 cgcagcagcg gatcataacc atactggcgg cggcaatagc ctacctcatc agccagtacg
     6841 tcaccgacag gctcatcgac ataccggatg agcgtgggat caaggacgac gtcctggagg
     6901 cggtcctcaa gggggcgacg acggcgacct cgaccatcct cgcctcggtc ctcgtgcgaa
     6961 ggttgttcag gggctgaacc tagcgggagc ctgtggaagc gccggagccc cgccgggttc
     7021 cggctttctt gtctgagatg gcgtatatca tcgtcggcgg gtgagggatc taggaggttg
     7081 attctggtgg caggtcctct ggagggccgg gtcgctatcg tgacgggcgg aggaggggga
     7141 ctggccgagg gcgtatgcgc caggctcgcc acagacggcg cggccgtagc ggcggtagac
     7201 atctcgcagg agaaggccga gagggtcgcc gggcaactca gggctgaagg cgccagcgcc
     7261 ggggccttcg aggccgatgt ctcagacagg cggtccgtcg aggccatggt cggtgaggtc
     7321 acgcgcgagt tcggcggggt ggacatcctc gtcaacaacg ccgccgtcta cccgcgacgg
     7381 gcgtggacgg agatcgagga ggaggagtgg gacagggtga tggccgttaa cctcaagggc
     7441 tacttcctgt gcgcgagggc cgtatatcct tacatgctgg agcgggggcg gggcaatatc
     7501 gtgaacgtgg cctcgatcac gttcttcatc gggtgggagc tgctgctcga ctacgttgcc
     7561 tcgaagggag gcatcgtcgg cttcacgcgc acgctcgccc gcgaggtcgg ccctgataac
     7621 attaacgtga acgccatagc accgggcgcc ttccccacgg acgcggagaa gatccacccc
     7681 gaccaggaag gctacaaccg ctgggtgct
//
LOCUS       DATAIR010000081         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_281726, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000081 DATAIR010000000
VERSION     DATAIR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281726"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..490
                     /locus_tag="VHM69_02130"
     CDS             320..490
                     /locus_tag="VHM69_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739218.1"
                     /translation="MKWELDLWYELWDRAGSQGLDTWHDIRGDARDTPASGRTPDASS
                     WEMPEERPPERT"
     gene            487..795
                     /locus_tag="VHM69_02135"
     CDS             487..795
                     /locus_tag="VHM69_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739219.1"
                     /translation="MTGLIAEAKERSYRKGGGFRGRRLGSELGSVVLASLLLTEVASS
                     IVGAEMVFFSALAVAWLILPAIVGLRNVLDAGCLFMLVSLGIVVVVDVAYHGPGSLLT
                     "
     gene            complement(842..>1666)
                     /gene="sat"
                     /locus_tag="VHM69_02140"
     CDS             complement(842..>1666)
                     /gene="sat"
                     /locus_tag="VHM69_02140"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX2739220.1"
                     /translation="MVLRELYGYDKEREARMVYRTTDADHPGVAAVYRQGDVLLGGEV
                     ELLRPPDEGRFPRYYFTPAQLRAEFARKGWKRIVGFQTRNPVHRAHEYIQKSALETVD
                     GLLLNPLVGETKSDDIPADVRMKSYEVILDRYYPEDRTMLAVFPAAMRYAGPREAIFH
                     AICRKNYGCSHFIVGRDHAGVGSYYGTYDAQHIFEEFDPEELGITPLFFEHAFFCKEC
                     GGMGSSKTCPHEPTSHVFLSGTKVREMLSKGEYPPPEFSRPEVIEVLMDGQRTESR"
BASE COUNT          314 a          485 c          514 g          353 t
ORIGIN      
        1 tacgaggcgt cgcgcagatc aaatacgaca tctcaggctt cacctcgcca ccgcctcagt
       61 tagtccgcga tcactcataa agcctcctca ttacgtcacc aatctctaca aaaataactt
      121 tgtctattac cataataaag aatattcgaa acctttgcca gagggacagg cgggaatgaa
      181 ggacccgtac gcggcaccgc gcggacggaa ttcccctgaa agaatgtcct gacacttgac
      241 gccgcgcaca ccttcattta ccctgtttgt acatttacaa tataggcaat ataaagaata
      301 tgaaaaggag gtagcggcga tgaagtggga gttggatctc tggtacgagc tttgggacag
      361 ggcgggcagt cagggtttgg atacctggca cgatatccgg ggcgacgcgc gggatactcc
      421 ggcgtccgga cgtacgcctg acgcctctag ttgggagatg cccgaagaaa ggcctccaga
      481 gaggacgtga ccggtctgat agcagaagcg aaggagcgct cgtaccgtaa gggcggcggg
      541 ttccgcgggc ggcggctcgg gtctgagctc ggatcggtcg tactggcttc gctgctgctc
      601 accgaggtgg cttcttcgat agttggggcc gagatggtct tcttttcggc tctcgcagtc
      661 gcctggctca ttctcccggc gatcgtgggg ctaaggaacg tcctcgacgc gggatgcctc
      721 ttcatgctcg tctctctcgg catagtcgtg gtagtcgacg ttgcctacca cgggccaggc
      781 tctctactga cttaatacag gatggaggac tcgcaaggct gcgggtcctc cattcccgct
      841 cctacctgct ctcggtccgt tgtccatcca tgagtacctc gataacttcg gggcgggaga
      901 actcgggggg cgggtactcg cccttggaga gcatttcgcg cactttcgtc cccgagagga
      961 agacgtggga ggtgggttcg tgcgggcagg tcttcgagga gcccatgccg ccgcactcct
     1021 tgcagaagaa cgcgtgctcg aagaagagag gtgtgatccc gagctcctcg ggatcgaact
     1081 cctcgaagat gtgctgggcg tcgtaggtac cgtagtagct gcccacgccc gcgtggtcgc
     1141 gccctacgat gaagtgcgag cagccgtaat tcttgcggca gatcgcgtgg aagattgcct
     1201 ccctcggacc ggcgtaacgc atcgccgccg ggaagacggc gagcatcgtg cggtcttccg
     1261 gatagtagcg gtccaggatc acctcgtatg acttcatcct gacgtcggcg ggtatgtcgt
     1321 ctgacttcgt ctccccgacg agcgggttga gaagtagccc gtccaccgtc tcgagcgcgc
     1381 tcttctggat gtactcgtgc gccctgtgga cggggttcct ggtctggaaa ccgacgatgc
     1441 gcttccagcc cttcctggcg aactcggccc ttagctgcgc cggcgtaaag tagtagcggg
     1501 ggaagcggcc ttcatcgggc gggcgcagta gttccacctc gccgccgagc agtacgtcgc
     1561 cctgcctgta gacagcggcc acaccgggat ggtcggcgtc ggtcgtcctg tagaccatcc
     1621 tggcctcgcg ctctttgtcg tatccgtaga gctccctgag caccat
//
LOCUS       DATAIR010000082         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_373732, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000082 DATAIR010000000
VERSION     DATAIR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373732"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      3..163
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            252..>1630
                     /locus_tag="VHM69_02145"
     CDS             252..>1630
                     /locus_tag="VHM69_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX2739221.1"
                     /translation="MSAPATAPETNYPRFKRWLLADRIEQVEGPYEKESKDRQQSWWK
                     VVCLTGVDYFSTLGYIPGIAALAAGVLSPIATLLIVALTVFAMLPMYRRVAEESPHGQ
                     GSIAILERLLSFWPGKLMVLALLGFIATDFIITITLSASDAAVHLAENPLVPGSLRDQ
                     EILITLVLVAALGAVFLKGFKEAINLAVVIVGVYLLLNLVVVAVGLYVMVTNPQSIAD
                     WRSVLFTNYGNPLVMIGVALLVFPKLALGISGFETGVTVMPLVRGEEGDDPEHPAGRV
                     RNTRKLLTSAALVMSFYLITTSFITVVLIPAREFEKGGSANGRALSYLAHEHLGGAFG
                     TVYDLSTIAILAFAGASAMAGLLNVVPRYLPRYGMAPEWGRAVRPLVLVFTAVALAIT
                     IDFGADVDAQGGAYATGVLVIMTSAAFAVTLSSLWDRDSLKGTLGFGLVNLIFAYTTV
                     ANVIERPDG"
BASE COUNT          260 a          547 c          512 g          311 t
ORIGIN      
        1 agacgccgag cccccgggtg gcgccggggg gcgggggacc cacatcggac ctctcaaagg
       61 tcctcggggt gaatcgccgg tcagctaacg ctctccggcg tagggcttaa ctctttcggc
      121 ccgaatccga cagctaacct cgtaggcgcc gaagaaggag gttctgcggt gtccgcgaag
      181 ctaccccgag cccgttcgtc gggcgtcttg gcttgcccgt cgcgagacgt gagccgtgcg
      241 cgaggcacgc gatgagcgcg ccagctacag cccccgagac caactatccc cgcttcaagc
      301 ggtggctgct cgccgatcgc atcgagcagg tcgaaggccc ctacgagaag gaaagcaagg
      361 atcgccagca gtcctggtgg aaggtggtgt gcctaacagg ggtggactac ttctccactt
      421 taggctacat ccccggcatt gcggcgcttg cggccggggt tctatcgccg atagccacgc
      481 ttctcatcgt cgcgctcaca gtgttcgcca tgctaccgat gtaccggcgc gtcgcggagg
      541 agagcccgca cgggcagggc tcgatcgcaa tcctcgagcg tctgctctcg ttctggcctg
      601 gcaaacttat ggtactagcc ctcctcgggt tcatcgccac ggacttcatc atcaccataa
      661 ccctctcggc ctccgacgcg gccgtccacc tcgccgagaa cccgctggta ccggggtcct
      721 tgcgagacca ggagatcctc atcaccctcg tgctcgtggc cgccctgggc gcggtgttcc
      781 taaagggctt caaagaggct atcaacctcg cggtcgtcat agtcggggtc tacctcctct
      841 tgaacctcgt cgtcgtagcc gtcggcctct acgtgatggt taccaacccc cagagcatcg
      901 ccgactggcg aagcgtgctc ttcaccaatt atggcaatcc gctggtcatg atcggggtgg
      961 ctttgctggt gttcccgaag ctcgctctgg gcatctcggg cttcgagacc ggcgtcacgg
     1021 tgatgccgct cgtgcgcggc gaggaggggg atgatccgga gcacccggcg gggcgcgtgc
     1081 gcaacacccg caagctcctg acctcagccg ccctggtcat gagcttctac ctgatcacca
     1141 cgagcttcat cacggtggtc ctcattcccg ccagggagtt cgagaagggc ggcagcgcca
     1201 acggccgggc gctctcctac ctggctcacg agcacctggg cggggccttc ggcacggtct
     1261 acgacttgag cacgatcgcg atcctcgcgt tcgccggagc ctcggcgatg gcgggactcc
     1321 tcaacgtcgt gccccgctac ctgccccgtt acgggatggc gccggagtgg gggcgggcag
     1381 tgaggccatt ggtgctggtc ttcaccgccg tggccctcgc tatcactatc gacttcggtg
     1441 ccgatgtgga tgcccagggc ggcgcatacg ctaccggggt cctggtgatc atgacgtctg
     1501 cagccttcgc cgtgacgctc tcgtcactgt gggaccggga ctccctcaag gggaccctcg
     1561 gcttcgggtt ggtcaacctg atcttcgcct acacgactgt cgccaacgtc atagagcgcc
     1621 cggacggcat
//
LOCUS       DATAIR010000083         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_373765, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000083 DATAIR010000000
VERSION     DATAIR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373765"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VHM69_02150"
     CDS             <1..890
                     /locus_tag="VHM69_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2739222.1"
                     /translation="LHHPLIFDPLKSVDTSSYPGDLLARAVRMGLSIYAAHTSYDAAP
                     EGVSVALAETLGLRGPLRVVSPRGALRKLVVFVPEEDLDAVARALAEAGAGVIGDYTE
                     CTFRTRGTGTFRGGEDANPYLGEKGRLEKFEEVRLETVVPAYAVRRAVDAATAAHPYE
                     EVALDVYPLEGFPEGCGYGRIGALPEPLAAEDLREHVSDVLGFPARLVADQGGRIDLV
                     AVLGGSGGSFIPEVAASGAHAYVTGDVDYHDALLAESLGLTVIDAGHAATELPSLQPL
                     AARLADLVDVPVAVSRVRR"
     gene            complement(887..1414)
                     /locus_tag="VHM69_02155"
     CDS             complement(887..1414)
                     /locus_tag="VHM69_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739223.1"
                     /translation="MIGPGDADRFSIKTARLCLRPVSMDDLDGLHRLWTDPAVREFFW
                     DGETISRERAEAAVREGMGAFDLHGFGLWIAEVGEDLIGFCGLRPLDNAPGVEVLYGI
                     TPLRWGEGFATEVALAMLRYGFEEAGLDRIIGIADRENAASRRVLEKIGMTFDEYVLN
                     EGREEAHYSVRWAPP"
     gene            complement(1411..2361)
                     /locus_tag="VHM69_02160"
     CDS             complement(1411..2361)
                     /locus_tag="VHM69_02160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX2739224.1"
                     /translation="MGKIVTLGEVVSDIYRGEESSAVEMGFVARPGGAPANVAVAAAR
                     LGSGAAFVGSVGEDLFGDFILRALESEGVDTTGVSRQSPPTRTSLAFVEIADDGDRAF
                     TFYRSNPAADELLSPEDVTRDLVSGASFVNFGSIPLIREPSRSATHRIAELANELDVP
                     VAFDVNFREHLWESAGDAREAVDPLLDLSTIVKLGDDELSPLLGTQEIEEAAGLLLDR
                     GVSLVLVSMGPEGAFYATRDFSGEVPTFDVGEVVDATGAGDAFLAAALVHLSGEPFDE
                     ERVREATLRGCAAGALACTDYGAMSALPTKDRLRRFMEDV"
     gene            complement(2392..>3178)
                     /locus_tag="VHM69_02165"
     CDS             complement(2392..>3178)
                     /locus_tag="VHM69_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX2739225.1"
                     /translation="MLPVHLHADDETARTKHGEANGKTEAWHIIWAAEGASILAGVRE
                     GVGRDELREAFLAQDHDSVMPRYPIRAGDTVYVPGGVIHAFGPDTLVFEVQQTSDLGQ
                     FVTPMSLYGERLDDDTWRSNIEAALDELRPDYRPRPNPGLVLQDDGNRRVVCCAGPHF
                     ALERWTLTEPLTEPSHPERCLTLSNVGEGVFIEYEGGTETLGSGESCVLPAAIGEVRV
                     TPESSEAILIACYVPDLERDIVAPLREAGYSDEEIRALGEVGI"
BASE COUNT          539 a         1085 c         1000 g          554 t
ORIGIN      
        1 tcctgcacca cccgcttatc ttcgatccac tcaaatcagt agatacgtcc tcatatcccg
       61 gggaccttct ggcccgagcc gtgcggatgg ggctctcgat ctacgccgcg cacaccagct
      121 acgacgccgc ccccgaaggc gtctcggtcg ccctggcaga aacactcggt ctccgtgggc
      181 cgctgcgggt ggtctcgccg cgtggggccc tgcgaaagct cgtggtgttc gtccccgagg
      241 aggacctgga tgccgtggcg cgggcgctcg cggaggccgg tgccggtgtg atcggagact
      301 acacagaatg tacgttccgt acacggggca cgggcacctt tcgcggcgga gaggacgcga
      361 acccgtacct cggggagaag ggcaggctcg agaagttcga ggaagtacgc ctcgagactg
      421 tcgtccctgc ctatgccgtg cggcgcgccg tagacgcggc caccgcggcc catccctacg
      481 aggaagtagc tctcgacgta tatccactcg aaggattccc tgaaggctgc ggttacggca
      541 ggataggtgc tctgccggag ccgctcgccg cggaggactt gcgcgagcac gtctcggatg
      601 tactgggctt ccctgcacgc ctggtcgctg accagggagg acgaatagat ctcgtcgccg
      661 tcctcggggg gtcagggggt tcctttatcc ctgaggtggc cgcttcgggc gcgcacgcct
      721 acgtgacagg ggatgtcgac taccacgacg ctttgctggc ggagtctctg ggcctcaccg
      781 tgatagatgc cggacacgcc gccacggagc tgccttcgct gcagcctctc gccgcgcggc
      841 tcgcggacct tgtggacgtg cccgtcgcgg tcagccgggt gcggcgctag ggcggagccc
      901 accggaccga ataatgcgcc tcttcgcggc cttcgttcag tacatactca tcgaaggtca
      961 tcccgatctt ctccagcacg cgcctggatg cggcgttctc tctatccgcg atcccgatta
     1021 ttcggtccag cccggcctcc tcgaaaccgt agcggagcat cgccagggcg acctccgtcg
     1081 cgaacccttc gccccagcgc aaaggtgtga tgccgtagag aacctccacc ccaggcgcgt
     1141 tgtcgagagg acgcaggccg cagaagccga tgaggtcctc ccccacttcc gcgatccaaa
     1201 ggccgaaccc gtgcagatca aacgccccca tgccctccct gacggcggct tcagccctct
     1261 ccctgcttat ggtctcgccg tcccagaaga attcccgcac cgcgggatcg gtccagaggc
     1321 ggtgcagtcc gtccaggtcg tccatcgaga ccggacggag acaaagtctg gccgtcttga
     1381 tagagaaccg gtccgcgtcg ccaggaccaa tcaaacatcc tccatgaacc gccgcagtct
     1441 atctttcgtg ggcagggcgc tcatggcgcc gtagtccgtg cacgcgaggg cgcctgccgc
     1501 gcagcccctc agggtagcct cgcgcacccg ctcctcgtcg aacggttcgc cggagaggtg
     1561 cacgagcgcc gctgcgagga aagcgtctcc ggcgccggtg gcgtcgacga cttcgccaac
     1621 gtcgaacgtg ggaacctctc cgctgaaatc tctggtggcg tagaacgcgc cttcaggacc
     1681 catgctcacc agcacgaggg agacgccgcg atccaacagc aatccggcgg cctcttcgat
     1741 ttcctgcgta ccgaggagcg gcgagagctc gtcgtcgcct agcttgacga tcgtggagag
     1801 gtcaagtagc gggtccaccg cttcccttgc gtctccggca ctctcccaca ggtgctcgcg
     1861 gaagttcacg tcgaaggcca cgggcacgtc gagctcatta gccagctcag cgatgcggtg
     1921 tgtcgccgaa cgggagggct cgcggataag gggtatgctg ccgaagttca cgaaagacgc
     1981 tccggagacc aggtctctag tgacgtcctc gggcgagagc aactcgtccg ctgcggggtt
     2041 ggatctgtag aaagtgaacg cgcggtcccc gtcgtcggct atctcgacga aggccagcga
     2101 ggtgcgcgtc ggcgggctct ggcggcttac gcccgtggta tccacgccct cggactcgag
     2161 cgcccgcagg atgaaatccc cgaataggtc ttccccgacg ctgccgacga acgcagcccc
     2221 cgagccgagg cgggccgccg cgacagccac gttcgcaggc gccccgccag gcctggcgac
     2281 gaaccccatc tcgacggcag agctctcctc ccccctgtag atatccgaaa cgacctcgcc
     2341 caatgtgact atcttcccca aagctcgctc ctgtctggtc ctcgaaggct cctagatacc
     2401 gacctcgccg agagcccgga tctcctcgtc cgagtacccg gcctcccgca atggcgccac
     2461 gatgtcgcgt tcgaggtcag ggacgtagca agcgatgagg atcgcctcgc tactttcggg
     2521 cgtcaccctc acctcaccaa ttgccgcagg caacacgcac gactcgcccg acccgagcgt
     2581 ctccgtcccg ccctcgtact cgatgaatac accttcaccc acgttggaga gcgtgaggca
     2641 acgctccggg tgggacggct ctgttaaggg ctcggtgagg gtccagcgtt cgagggcgaa
     2701 gtgcgggccg gcgcagcaga ctacgcggcg attcccatcg tcctggagga cgaggccggg
     2761 gttcgggcgc ggccggtagt cagggcggag ttcgtcgagg gcggcctcga tgttcgacct
     2821 ccaggtatcg tcgtccaggc gctcgccgta gagactcatg ggcgttacga actggccgag
     2881 gtccgaagtc tgctggacct cgaatacgag cgtatccgga ccgaaggcgt ggatcacacc
     2941 tccaggtacg tacaccgtgt cgcccgccct tatcgggtag cgcggcatga cagagtcgtg
     3001 atcctgcgcc agaaaagcct cgcgcaattc gtccctgccg acaccttctc tgacgccggc
     3061 caggatcgag gccccctccg ccgcccagat gatgtgccac gcctcggtct ttccgtttgc
     3121 ctcgccgtgc tttgtgcgcg ccgtctcatc gtcagcatgc aggtggacgg ggagcatg
//
LOCUS       DATAIR010000084         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_5017, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000084 DATAIR010000000
VERSION     DATAIR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..652
                     /locus_tag="VHM69_02170"
     CDS             113..652
                     /locus_tag="VHM69_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2739226.1"
                     /translation="MSGRPIGMEQQAPLACLPYDPRAPEVASTVTGMIHQHLPHVLVE
                     HIGSTAVQGCVGKGVIDLLIPYREGELEPIKETLQELGFQRQSRRDPFPEDRPMRVGS
                     IRYDGDTFRLHVHVVPASSGEPDELRAFRDRLRNDPEMMEAYVRRKREIIQGGTTDAI
                     DYSIIKGAFVQEALEEMNR"
     gene            complement(675..1049)
                     /locus_tag="VHM69_02175"
     CDS             complement(675..1049)
                     /locus_tag="VHM69_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739227.1"
                     /translation="MPEYLNQLLADSATALVNESFTGVSAPWWWERRMGGGIEVCQEL
                     DPEAMSREISERTGHEASEVRRAIAEVLGLEDAEPVVLTFEIAGETETRQVAQMLAER
                     SSAPEGLASGLYRRVEEVVKTS"
     gene            1132..1782
                     /locus_tag="VHM69_02180"
     CDS             1132..1782
                     /locus_tag="VHM69_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX2739228.1"
                     /translation="MLLVKDSMTREIVTISPDETAEAALAACRERRIRHLPVLTEGRL
                     VGIVSDRDLRSAIPVLGDPARAAALREMLVKDVMVGDVVTALPDDPIEHAANVMREMR
                     IGCLPVVEGGELVGILTSSDVMAALVYLVGANEPGSRMEIALPDRPGSLAGAAGVFGM
                     CGINIVSVAMGATREPTEEGAPKERVVVFRVDTIDTAEVVGYLEEAGYPVLWPPKT"
     gene            1779..2939
                     /locus_tag="VHM69_02185"
     CDS             1779..2939
                     /locus_tag="VHM69_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX2739229.1"
                     /translation="MSGLAAIVHDLALEDYGFGGDHPFNPLRVRLALELCEALELLED
                     YPFISSEPATDEDLTTVHSLTYVRMVQDAGRSQGALADLMQYGLGTPDNPVFPDMHEA
                     CARVVGGVLAACRLVMRGEATHAMCISGGLHHALRSRASGFCVYNDAGVAIARLKQEH
                     PGIRVAYVDTDAHHGDGVQWMFYSDPDVLTVSMHESGRYLFPGTGEVGENGRNEGTGY
                     SVNVPLEPFTDDGSWISCFEAVVPDVLRAFGPDLILSQNGCDGHTLDPLTHLSATTRF
                     YEHVPNRMHDLAHDLCEGRWVATGGGGYDIWRVVPRAWTALWAAVSHQELPEKVSEDW
                     LSKWGAKSPVKLPRLMRDDPVDYPQGPRAAHIASRNERTVEEVIENVIPLIR"
     gene            3018..3347
                     /locus_tag="VHM69_02190"
     CDS             3018..3347
                     /locus_tag="VHM69_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX2739230.1"
                     /translation="MQTISPLHDVAIRSASSTETKPGIEIERSVTEEPPYKVILHNDD
                     YTPMEHVVAVLQKVIPRMSVRRAVDIMVEAHTKGKAVATKCHKELAELYQEGLNNEGL
                     IASIEPD"
BASE COUNT          679 a         1068 c         1076 g          605 t
ORIGIN      
        1 aagtgtaggt ttcaagaatg tcctcaacct cgaaggcggc ctcgacgcct actccttcat
       61 tgacacagcc gtttcgaggt actgaggtgt ccccaaaggc ggcgttggat ccttgtccgg
      121 gcgtcccata gggatggagc agcaggctcc gctggcatgc ctgccctatg atcctcgcgc
      181 ccccgaggta gcgagcacgg ttacggggat gatccaccag caccttcccc acgtgctcgt
      241 cgagcacatc ggtagtaccg cagttcaagg gtgcgtcggg aagggtgtga tcgaccttct
      301 cataccgtac agggaaggcg aactcgaacc catcaaggag acactacagg agctaggctt
      361 ccaacgtcag agcaggagag atcctttccc cgaagacagg ccgatgcgtg tgggatccat
      421 tagatacgac ggggacacgt tcaggctcca cgtccacgtc gtaccagcga gttccggaga
      481 gcccgacgaa cttcgcgcct tccgcgacag gctgcgcaac gacccggaga tgatggaagc
      541 atacgtccgg cggaagagag aaataataca aggcgggacg acggatgcga tcgactactc
      601 gataatcaag ggcgcgttcg ttcaggaagc attggaagag atgaaccgct agattcgctc
      661 cctgatccag gaacctaact ggtcttgacc acctcttcga cgcggcggta gaggccggac
      721 gccagaccct ccggtgccga ggagcgttcg gcgagcatct gtgcgacctg gcgggtctcg
      781 gtctcgcctg caatctcgaa ggtgaggacg acgggctccg cgtcctcgag gccaagcact
      841 tcggcgatgg cccgacggac ttcggaggct tcgtggcctg tccgttcgga gatctcacgg
      901 ctcatggctt cggggtcgag ttcctggcag acttcgatgc cgcctcccat gcgccgttcc
      961 caccaccacg gggcgcttac gcccgtgaag ctctcgttca ccagggccgt cgcgctgtcg
     1021 gcgaggagtt gattgaggta ttctggcatg aggacgttat accagtcgca ggcttacttg
     1081 cgatgatcca ctgctataca atgccatgag ggaaggagtt gagataatcc gatgcttctc
     1141 gtaaaggact cgatgacccg cgagatcgta acgatctcgc ccgatgagac ggcggaggct
     1201 gcgctcgccg cgtgcaggga gaggcggatc aggcacctgc cggtcctgac ggaggggagg
     1261 ctcgtcggga tcgtctcgga tagggatctg cgctcggcca tccctgttct cggagacccc
     1321 gcgcgcgccg cggccctgcg ggagatgctt gtaaaggacg tgatggtagg cgacgttgtg
     1381 acggcattgc ccgatgatcc catagagcac gcggcgaacg tgatgcgcga gatgaggatc
     1441 ggctgcctcc ccgtggtcga gggcggcgag ctggtcggca tattgacttc ctcggacgtg
     1501 atggccgcgc tggtctacct ggtcggcgcc aacgagccag ggagccgcat ggagatcgca
     1561 ctccccgaca ggcctgggtc cctcgccggc gcggcgggcg tcttcgggat gtgcggcata
     1621 aacatcgtga gcgtcgcaat gggggcaacg cgggagccca cggaggaagg agcgcccaag
     1681 gagcgcgtcg tcgtgttcag ggtggatacc atcgatacag ccgaagtggt cggatacctc
     1741 gaggaggcag gatatcctgt tctctggccg ccgaagactt gagcggcctg gccgccatcg
     1801 tccacgacct ggccctggag gactacggat tcggcggtga ccatcctttc aacccgcttc
     1861 gtgtcaggct cgccctggaa ctctgcgagg ccctcgaact gctggaggac tatcctttca
     1921 tctcctcgga gcccgcgacg gacgaggacc tgacgaccgt ccacagcctc acatacgtgc
     1981 gaatggtcca ggacgcgggc aggtcccagg gcgcgctcgc ggacttgatg caatacggtc
     2041 tcgggacccc ggataacccc gtctttcccg acatgcacga agcctgcgcc cgtgtggtgg
     2101 ggggagtact cgcggcgtgc aggctcgtga tgaggggaga ggctacccac gccatgtgca
     2161 tctcgggcgg gctgcaccac gcccttcgct ccagggcgag cggcttctgc gtctacaacg
     2221 acgccggcgt cgccatcgcg cggctcaaac aggagcaccc cggcatccgc gtcgcctacg
     2281 tcgacaccga cgcccaccat ggtgacgggg tgcagtggat gttctacagc gaccccgacg
     2341 tcctgaccgt ctccatgcac gagtcgggcc gctacctctt tcctgggaca ggcgaggtgg
     2401 gggagaacgg acgcaacgaa ggcacgggct actcggtcaa cgtaccgctc gaaccattca
     2461 cggacgacgg gtcctggatc tcctgcttcg aggccgtcgt ccccgatgtc cttcgcgcct
     2521 tcggtcccga cctgatcctc tcccagaacg gttgcgacgg ccacacgctc gaccccctga
     2581 cacacctctc ggctacgacc cgcttctacg agcacgtccc caacaggatg cacgacctcg
     2641 cccacgacct ctgcgagggc cgctgggtcg caactggcgg cggtggctac gacatctggc
     2701 gcgtcgtccc gcgcgcctgg acggccctct gggccgccgt ctctcaccag gaattacctg
     2761 agaaagtatc cgaagactgg ctctcgaagt ggggagcgaa gagccccgtg aaattgcccc
     2821 gcctcatgcg cgacgatccc gtggattatc cgcagggacc gcgcgccgct catatagcga
     2881 gccggaacga acgtaccgtc gaagaggtca ttgagaacgt gatccccctg atccgttaga
     2941 gaagaaaaag ggttgcgcat ccggcgtcgt cgcggtattt tgccttgagt gatcaacact
     3001 tcggaccgga gatgacagtg caaaccatat caccgttgca tgatgtggcg atccgttcag
     3061 cctcttccac agagacgaag ccggggatag agattgagcg cagcgtcacc gaggagccgc
     3121 catacaaggt catcctgcac aacgacgact acaccccgat ggaacacgtc gtcgccgtgc
     3181 tgcagaaagt catcccgcgc atgtccgtgc ggcgcgccgt tgatatcatg gtggaggccc
     3241 ataccaaagg caaagccgtc gccaccaaat gccacaaaga gctcgccgag ctctaccagg
     3301 agggactaaa caacgagggc ctcatcgcct ccatagagcc tgactagact acctcaggag
     3361 cacgccattg aaggttgagg aggccgaagt actgctgaga aattacctgc tcaacggcct
     3421 cgcgatgg
//
LOCUS       DATAIR010000085         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_5164, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000085 DATAIR010000000
VERSION     DATAIR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5164"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1556)
                     /locus_tag="VHM69_02195"
     CDS             complement(33..1556)
                     /locus_tag="VHM69_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2739231.1"
                     /translation="MNEEPLLIGADVGTTNIKVVAFDRSGRTVAGASSHTPTRYPRPG
                     RAHYDPDELWDTFAATLRRVMEKLDEPGRVASIAVASTGEAAVPLDSGGRPAYDIITW
                     FDGRARPQSERLGRVVGQDRLFGLTGLSLQPIFGLCKLLWLKENEPDAYSRTASWLNV
                     ADYMAFRLCGVAATDYSLASRTLALDLHNLRWAGELLEEVGISPDLFAPLREGGSFLG
                     PVTPEAASATGLPESAGVATGGHDHVCGALAVGITQTGTMLNSLGTAEAIFMPLERPL
                     TDPRVGHQGYTQGAHVAGRYYVFGGQYTSGASVEWLREALGGVDYDTLVSEAEGVPPG
                     SLGAFFLPHLRLANPPHDDPASRGAFVGLSTDVKSKELFRAVLEGLAYDSRNSLEPLL
                     AHSGVDRLRAIYAIGGGTQNRLLMRIKATVLNQEITVVGVEEATSLGAAILGGIGAGV
                     YADIPSALEELRYSESPVEPVPDEVPFYDAAFRRVYQRIYPSVRGVHHEIQDLQSDR"
     gene            1726..2760
                     /locus_tag="VHM69_02200"
     CDS             1726..2760
                     /locus_tag="VHM69_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX2739232.1"
                     /translation="MLFLASVGLAVHLILPQIPGLERSARLVAEAAPALIFAAFVAEF
                     MSEASYAELLGRSVAAASRVGTSARSRSRRGLGPWFMLRLTICGYGAAHVLPGGGAAA
                     ATVTYRALRARGVGRSSVGLAVASVSGLVYGALGVMFSGSLLYMLVLGDLTQAEEDLI
                     FVTAVLVVAASIAGYVAYRKPRVARRMLGDTVFRVIRLVRRRYGRREAGEIVDRLGEG
                     LRSLRRQVRSRPLEAVGWCCLAFAYWAFDALCLVLVFLALGVSAGTGELLVAYGIATA
                     AGALPLTPGGIGVFEATMLATLAVFGVGAEAAIPVLGYRLFNFWLPIPLSALLYPTLR
                     IGAGSEGGVR"
     gene            2798..>3021
                     /locus_tag="VHM69_02205"
     CDS             2798..>3021
                     /locus_tag="VHM69_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX2739233.1"
                     /translation="MIVDIAIYVEGRRTEPSELEGIRRTCRERGGFARIGLVDPDEEE
                     FASVSRELDLHEMAVRDAIKSHQRPKLERYG"
BASE COUNT          452 a          949 c         1074 g          546 t
ORIGIN      
        1 aagtggcagc ggtggttcac gaggtaggga agttaccggt cggattgcag gtcctggatc
       61 tcgtggtgca cgccgcggac gctcgggtat atcctctggt aaaccctgcg gaaggcggcg
      121 tcgtagaacg ggacctcgtc cggcaccggc tcgacggggc tctcggagta gcgcaactct
      181 tcgagggccg agggaatatc ggcgtagacg ccggcgccta tcccgccaag gatggctgca
      241 ccaaggctgg tcgcctcctc cacaccgacc acggtgatct cctggttcag gacggtggct
      301 ttgatgcgca tgagcagacg gttctgggtg ccgccgccga tggcgtagat ggcacgcaac
      361 cggtcaaccc ctgagtgggc gaggagcggt tcgagcgagt tgcgtgagtc gtaggccaga
      421 ccttcgagca cagccctgaa tagctccttg ctcttgacgt ccgtgctcag gccgacgaac
      481 gctcccctcg atgcgggatc gtcgtgcggc gggttcgcga ggcgcaggtg cggcaggaag
      541 aaggcgccca gagatcctgg cggaacccct tccgcctcgg agacgagggt atcgtagtcc
      601 accccgccca gggcctcgcg caaccactcg acgcttgcgc ccgaggtgta ctggcctccg
      661 aatacatagt accggccggc cacgtgggcg ccctgggtgt acccctgatg cccgacgcgg
      721 gggtccgtga gcgggcgctc caacggcatg aagatggcct ccgcggtacc gagcgagttc
      781 agcatcgtgc ctgtctgcgt gatgccgacg gcgagcgcgc cgcagacgtg gtcgtggccg
      841 ccggtcgcga caccggcgct ctcggggagc cccgtcgccg aggcggcctc gggggtcacg
      901 gggccgagga acgagccgcc ctctctcagg ggcgcgaaga gatcagggga gatgccaacc
      961 tcctccagca actccccggc ccaccgcagg ttgtgcaggt cgagggcgag cgtgcgcgag
     1021 gcaagcgagt agtccgtggc ggccacgccg cagaggcgga acgccatgta gtcggcgacg
     1081 ttgagccagg aggctgtgcg cgagtaggcg tcgggctcgt tctctttgag ccatagcagc
     1141 ttgcacaggc cgaagatggg ttgcaaagag aggcccgtca ggccgaagag gcggtcctgt
     1201 ccgacgacgc ggccaagccg ctcggactgc ggccgcgcgc gcccatcgaa ccaggtgatg
     1261 atgtcgtagg cgggccggcc gccggaatcc agcggcacgg ccgcctcgcc tgtgcttgcg
     1321 acggctatgc tcgccacgcg gcccggctcg tcgagcttct ccatcacccg gcgcagggtc
     1381 gccgcgaaag tgtcccacag ctcatcaggg tcgtagtgag ccctgcccgg tctcgggtat
     1441 cgggtgggag tatgagagct ggcccccgct acggtcctgc cgctcctgtc gaaggcaacg
     1501 accttgatgt tcgtcgtgcc tacatcggcc ccgatcagga gcggttcctc attcatcgga
     1561 ttggccaacc tctcaagatc cggcgccttc tcgcaaggtt tcctatcata ctgtgagtgg
     1621 aagttcgacg agagtacggc atacacggag taccctgtgc actatgctat gtcgcgtgtg
     1681 aagaatagca gaggggcacg gatctccagg actgttttgc gcaatgtgct gttcctggct
     1741 tcggtggggt tggccgtaca tctgatcctg ccccagatcc cgggcctgga acgctcggcc
     1801 aggctcgttg ccgaggccgc gccggctctg atattcgctg cgtttgtggc cgagttcatg
     1861 agcgaggcga gctacgcgga gttgctcggg cgctccgtgg cggctgcgtc gagggtgggg
     1921 acgtccgcgc ggtccaggag ccgcaggggt ctcggcccct ggttcatgct gcgcctgacg
     1981 atctgcgggt acggcgccgc ccacgtgctg ccaggcggag gtgccgccgc cgcgaccgtg
     2041 acctaccggg cgctgcgggc ccgcggggtt ggccgcagct ccgtcggcct cgcagtggcc
     2101 tcggtctcgg ggctcgtgta cggggcgctc ggcgtcatgt tctcagggag cctcctttac
     2161 atgctagtgc tgggcgatct gacgcaggct gaggaggacc tcatatttgt gaccgcagtg
     2221 ctcgttgtgg ctgcttccat cgccggttac gttgcctaca ggaagccgcg cgtcgcccgg
     2281 cggatgctcg gggacacagt cttcagggtg atccggctcg tgcgccgccg gtacgggaga
     2341 agggaggccg gcgagatcgt tgatcgcctg ggtgaggggc tgagatctct gcggcggcag
     2401 gtccgaagcc gcccgctgga ggccgttggg tggtgctgcc tcgccttcgc ttactgggcg
     2461 ttcgacgctc tgtgtctcgt cctggtattc ctggcgctgg gagtatccgc gggtaccggc
     2521 gagctgctcg tcgcctacgg catcgccacg gcggcaggcg cgctccctct gacaccgggc
     2581 ggcatcgggg tgttcgaggc caccatgctc gccaccctcg ccgtgttcgg ggtcggggct
     2641 gaggccgcga tcccggtcct cgggtaccgc ctctttaatt tctggctgcc catccccctc
     2701 tccgcactcc tgtacccgac cctgcggata ggagctggaa gtgaaggcgg agtgagatag
     2761 atcctttcgt cgggcgcccg gcgtataatg tgcgcccgtg atcgtggaca tcgccatcta
     2821 cgtcgagggg cggcggacgg aaccttccga gctggagggg atcaggcgta cctgcagaga
     2881 gcggggcggg tttgcccgga tagggctcgt cgatccggat gaggaggagt tcgcctccgt
     2941 gtcccgcgag ttggacctgc acgagatggc cgtgcgcgat gcgatcaagt cccaccagcg
     3001 tcccaagctg gagcgttacg g
//
LOCUS       DATAIR010000086         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_233129, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000086 DATAIR010000000
VERSION     DATAIR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233129"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2729)
                     /locus_tag="VHM69_02210"
     CDS             complement(<1..2729)
                     /locus_tag="VHM69_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2739234.1"
                     /translation="MRGRGAERGAAELERVPAVTYVQEVGHETASYISPQMEALTGYS
                     KKDFEKDPGLWYGAIHPDDIGRVIAEDERTDETGEPFSMEYRMVRRDGGVVWVRDEAV
                     LAEDHDGRHRLWQGVMIDVTERKRIEAALHESEQRFRGAFENASTGVALVGLDRRFLR
                     VNRALREMLGYSEEELLRKTSPEITHPDDRELSRDRSERLFAGDAGAEIIEKRYVRKD
                     GETLWVLSSVSMVRDPEGKPSHFVAQFQDITQRKGVEEAIKESEERFRALVQNASDIV
                     AIFEADGTVRYVSPAVEQVLGHRPEDLIGKSSFAIMHPEDVPRARQFLGDVVGRPGVT
                     FSIDLRLRHVDGSWRHFESRCTNLLGDPAVRGIVINSRDVTERKEAEARYRTLVEQIP
                     AGVYIQEIEHEGAISYISPRIEDILGYTAREYVENPGLWIETTHPEDREKVLAEDTRT
                     DATGGPFRMEFRKISREGRLVWIRDEAVLVRDSEGNPLQWQGLFVDITERKEAEERLR
                     ESKRRLSTLLSNAPAYLYRCRNEPGWPNEFVSDYAFELTGYTPDELTDGRVMFDDLIV
                     EEDRDRIWDEVQAALAASEGFELRYSVRRKDGEVRHVEERGQGIYGEDGDVEAIEGVV
                     YDVTERVRAEARLKEAEARYRTLVEQIPAVTYIDHADGSFESLYTSPQIEEMLGYTQE
                     EWRANKLWESSLHPDDRDRVLTADDRLEIEGEPFSEEYRLLAKDGSVVWVREDAVLIK
                     DAEGGPLYWQGVIFDMTERKEAEEELRKSQANLAEAQRMAHLGSWEWDLITGEVDWSD
                     EVFRIYGFEPQSFAPTFERLLDVVHPEDRKTLRKAVEGALEEGLPYDYEHRIVKPDGE
                     VRVVHRRAEVVRAEDGEPITMVGTVHDVTERKALEGQLQHQALHDPLTGLPNRT"
     gene            3025..3097
                     /locus_tag="VHM69_02215"
     tRNA            3025..3097
                     /locus_tag="VHM69_02215"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3058..3060,aa:Ala,seq:cgc)
BASE COUNT          590 a         1191 c         1004 g          728 t
ORIGIN      
        1 gtcctgttgg gcaaacccgt cagggggtca tgcagcgcct gatgctgaag ttggccttca
       61 agggccttgc gctcggtcac gtcgtgtacg gtgccgacca tcgtgatcgg ctcgccgtcc
      121 tccgcgcgca caacctcagc ccgacggtga accaccctga cctcgccgtc aggcttgact
      181 atgcggtgct cgtagtcgta aggtagcccc tcctcgagag cgccttcgac cgccttccga
      241 agtgtcttcc tgtcctcggg atgcacaaca tcgaggagcc tctcgaaggt gggggcgaaa
      301 gactgtggtt cgaagccgta gatgcggaag acctcgtcgg accagtcgac ctcgcccgtt
      361 atcaggtccc actcccaact cccgaggtgc gccatgcgct gcgcctcggc caggttcgcc
      421 tggctcttgc gaagctcctc ttcggcctcc ttgcgctcgg tcatgtcgaa gatgacgccc
      481 tgccagtaaa gaggcccgcc ctccgcgtcc ttgatcagga cggcatcctc ccgcacccac
      541 accacggacc cgtccttcgc cagcaaccgg tactcctcgc tgaaaggctc gccttcgatc
      601 tccaggcggt cgtccgcagt caggaccctg tcgcggtcgt cggggtggag gctactctcc
      661 cacaacttgt tcgcccgcca ttcttcctgg gtgtagccga gcatctcctc tatctggggg
      721 ctggtgtata aagactcgaa cgagccatcg gcatggtcta tgtaggtcac ggcgggtatc
      781 tgctcgacca gggtcctgta gcgggcctcg gcctccttca gcctcgcttc cgcccgcacg
      841 cgttccgtaa cgtcgtagac gacgccctcg atagcctcca cgtccccgtc ctcgccgtag
      901 attccctgac cgcgctcctc cacgtgcctt acctcgccgt ccttgcggcg taccgagtac
      961 cgcagttcga agccttcgga cgcggccaac gcagcctgca cctcgtccca gatcctgtcc
     1021 ctgtcctcct ccactatgag atcgtcgaac atgactctcc cgtcggtgag ctcatccggc
     1081 gtgtagcctg tcagttcgaa ggcgtagtcg ctgacgaact cgttgggcca ccctggctcg
     1141 ttgcgacaac ggtaaaggta ggcaggggcg ttggagagca gtgtcgagag gcgccgcttg
     1201 ctctccctga gcctctcctc agcctccttg cgctccgtga tgtcgacgaa gagaccctgc
     1261 cattggaggg gattaccctc ggagtccctc acgaggacag cctcgtcgcg tatccagacg
     1321 aggcggcctt cgcgtgagat cttcctgaac tccatcctga aaggcccgcc tgtcgcgtcc
     1381 gtgcgggtat cttcagcaag gaccttctcg cggtcctcgg ggtgcgtggt ctcgatccag
     1441 aggccgggat tctctacgta ttcccgcgcg gtgtagccga gtatgtcttc gatcctcggg
     1501 ctgatgtagg agatagcacc ttcgtgctct atctcctgga tgtagacgcc ggcgggtatc
     1561 tgctcgacca gtgtcctgta gcgggcctct gcctccttgc gttcggtgac gtcgcgggag
     1621 ttgatcacga tccctcgcac cgcgggatct ccgaggaggt tggtgcaacg cgactcgaag
     1681 tgccgccacg agccgtccac gtgcctcaga cgcagatcga ttgagaaggt gaccccaggc
     1741 cgccccacga catcgccgag gaattgccgc gcgcgcggta cgtcttcggg atgcatgatg
     1801 gcaaagctgc tttttcctat cagatcctcc ggcctgtgtc cgagcacctg ctcgaccgcg
     1861 gggctcacgt agcgcacggt tccgtcggct tcgaatatgg cgacgatgtc cgaggcgttc
     1921 tgcacgaggg cgcggaaccg ctcctcgctc tccttgatcg cctcctccac ccccttgcgt
     1981 tgcgtaatgt cctggaactg ggcgacgaaa tggctcggtt tgccctcggg atccctcacc
     2041 atcgaaacgc tcgacaacac ccacaaggtc tccccgtcct tgcggacgta gcgcttctcg
     2101 atgatttccg caccggcatc gccggcgaag agacgctcag agcggtccct gctcaactcg
     2161 cggtcgtcgg ggtgggtgat ctcgggcgaa gtcttgcgga ggagctcttc ctcagaatag
     2221 ccgagcatct cgcgcagcgc cctgttgacc ctcaggaagc gcctgtccag gccgacgagc
     2281 gcaacacccg tggaggcgtt ctcgaaggcg ccacggaagc gttgctcgct ctcgtgtagc
     2341 gcggcctcga tccgcttgcg ttccgtcacg tcgatcatca cgccctgcca caggcggtgg
     2401 cgtccgtcgt gatcttcggc cagcacggct tcgtcgcgta cccacaccac cccaccgtcg
     2461 cgacgcacca tacgatactc catgctgaaa ggctcgcccg tctcgtcagt gcgctcgtcc
     2521 tcggcgatca cacgccctat atcgtcaggg tgtatggcac cgtaccagag ccctggatcc
     2581 ttctcgaaat ccttcttcga gtagccggtc aacgcctcca tctgcgggct gatgtacgaa
     2641 gcggtctcgt gtccgacttc ctgtacgtag gttacggcag gcacacgttc gagctcggcc
     2701 gccccgcgtt ccgcacccct gcccctcaat ctcctgagca agttgcgtaa gatcatcatc
     2761 aaattagagg cccgctatta gttgcgatat gatagcgacc gatgtagtcg ccgcatcctc
     2821 catgcagaaa ccgctttccc atgcaagtat tctatccgat cacagcgccg cagacgtgcg
     2881 gtcggaccgc tgcatccccg agattccagg cctcccagcg agagatagcg gacgcatcct
     2941 caccggagaa gatccctcct cccatccccc tgtacctcca ccttgcaatc caggtgccgg
     3001 atgctaggat tgctgaggct tcacgggccc gtagctcagc cgggagagcg ccgccctcgc
     3061 atggcggagg tcacgagttc gagtctcgtc gggtccatac tctttttgtt ggcttttgca
     3121 ggcaaaacgt ggagcactct agaaaccctg ttcaacagca ctagatacag ggacgagttc
     3181 caggatgtag gcgcattgcg ggtacagtat gttctccccg cgcccctacc aggtggggca
     3241 cgacctggga gcaaaaagcg ccggcagatt aagcgcaaag gtgggtcccg gaaagctctt
     3301 gcgaagctag acccggaggg cggccatcaa ggccgggttt cagggtgccc ttgcgtagac
     3361 ggctgccggt gccaggctat aggccctctg cgcgctctct gccgcatgat cctgcgaaca
     3421 aggtctctcc tcgctcgtgc cagctaccta cccggtcagc agcttcccag cattcttcat
     3481 ccataggcgt tcgcataggg actctgggcg ccc
//
LOCUS       DATAIR010000087         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_48413, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000087 DATAIR010000000
VERSION     DATAIR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48413"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VHM69_02220"
     CDS             <1..1395
                     /locus_tag="VHM69_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739235.1"
                     /translation="YLTQWAMFRNGLRQEVAGTLVLGEPADGTWMILQSGGYYGATLL
                     LFLLYLWLLILCRSGLLRDGRTRTLALGFPVLFNAGLLFGDPYQSIDALTYIAHGYMG
                     NLPDVNPYVTNAAEVARFDFAGQLAPYGWLPVHGFSPYGPLWTYLEIAAFKVAGDVSS
                     TLLLIKSMVVASSLGSAALIWKILGRVRPEDQLLGTLVYLWNPVIVVEFAAEGHNDAL
                     MILCVLASLALCVSWRPAPTMVASVLGVLVKYVPVIFVPAQVVYLWHSRRSRMRLALS
                     IFVGLLIGVGLAFLIYDPFWVGLRTFEGVRVQGQPLYAPSPSGLLYGYLSLAYPQGVA
                     DRLTLWVLAGAFAVFVLFASLRARDAAGLFGACACIALAYVLVVSSHYWPWYASLPLA
                     LMALRPGGIFFPAALVLTLCSRLVAPLSTLYTNDFISAEFSLDLKMAVGVTGPLAVLL
                     LLSLLPRFRREAKE"
     gene            1491..2057
                     /locus_tag="VHM69_02225"
     CDS             1491..2057
                     /locus_tag="VHM69_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739236.1"
                     /translation="MELDWLKDWVQIAQGLATIAAILIAGIWAYYVFAVGRSATAQVQ
                     ITCHLKEVIASPGGKILCISVMLTNTGRTRVADLSCEIIAAPVANSGDDRAALALLEP
                     LPERILDEGEPGSDFAWGKMFRETVGLEPNEAVAEDILLSVRAGSPASADDPGAFAAE
                     VHCRGNILGGRKGDWGWRGILDSRKVGE"
     gene            2171..2416
                     /locus_tag="VHM69_02230"
     CDS             2171..2416
                     /locus_tag="VHM69_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739237.1"
                     /translation="MIFEIVEVLGRSGDIDEQTRTAILDGVHELRRDMLGASDEKLEE
                     WSTSVERCMKMSSVEFGSGLSQASAEDLRGCLDGIRH"
     gene            complement(2413..2769)
                     /locus_tag="VHM69_02235"
     CDS             complement(2413..2769)
                     /locus_tag="VHM69_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739238.1"
                     /translation="MPTTVRIRPEVITAHRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     RGWVYAGEPDFIYRQIREVVIALPDITFEPDAIRESVKTVIEKARTEEEREEGRLLLR
                     RAFDKTGQLAEAEEFL"
     gene            complement(2804..>3141)
                     /locus_tag="VHM69_02240"
     CDS             complement(2804..>3141)
                     /locus_tag="VHM69_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2739239.1"
                     /translation="FLDLPLPPARLLADEGAILALATDFNPGSSFCESLPLVMNLACT
                     RLGLSPAEALCACTANAAHVLGLEDVGRLAPGYMADVLLLDAPDWRHVAYHLGGDRFA
                     AKIKSWRIL"
BASE COUNT          518 a          933 c         1020 g          670 t
ORIGIN      
        1 tacctcacgc agtgggcgat gttccgcaac gggctgcgcc aggaggtcgc cggcacgctc
       61 gttctggggg aacccgccga cggcacatgg atgatcctcc agagcggcgg ctattacggg
      121 gcaacactac tcctcttcct actctatttg tggctcttga tcctctgccg cagcggcttg
      181 ctcagggatg gccggacacg cacgctcgcc ctcgggttcc ctgtcctgtt caacgccggg
      241 ctgctgttcg gggatccata ccagtcaatc gacgccctca cctacatcgc ccacggctac
      301 atgggcaatc tgccggacgt aaacccctat gtgacgaacg ccgcggaggt cgcgcgtttt
      361 gacttcgcag gccaactcgc gccctacgga tggctccccg tacacggctt ctcgccttac
      421 ggacccctgt ggacgtacct cgagatcgcc gcgttcaagg tcgcgggcga cgtgtcgtcc
      481 acgctgcttc tgatcaagtc gatggtggtg gcttcgagcc tcggttctgc ggccctcatc
      541 tggaagatcc tcggccgcgt gcgtcctgag gatcaactcc tcggcacgct cgtctacctc
      601 tggaaccccg tgatcgtcgt cgagttcgcc gcagaaggcc acaacgacgc gctcatgatc
      661 ctgtgcgtgc tggcctcgct cgcgctttgc gtctcgtggc gaccagcgcc gacgatggtg
      721 gcctccgtgc tgggggtgct cgtcaagtac gtgcccgtga tcttcgttcc cgcgcaggtt
      781 gtctacctct ggcattccag gcggagccgg atgcggcttg ctctctccat cttcgtcggg
      841 ctcctgatcg gggtcgggct cgccttcctg atctacgacc cgttctgggt gggattgcgc
      901 actttcgagg gcgtgcgcgt tcaaggacag ccgctgtacg ctccttcgcc ttccgggttg
      961 ttgtacgggt atctttcgct cgcgtatccg cagggagtgg ccgaccggct cacgctgtgg
     1021 gttctggccg gcgcttttgc tgttttcgtg cttttcgcca gccttcgtgc gcgcgacgcc
     1081 gcgggattgt tcggggcgtg cgcctgcatc gcgctggcct acgtgctcgt agtctcgtcc
     1141 cattactggc cctggtatgc gagccttccg ctggcgctca tggctctcag gcccggcggc
     1201 atcttcttcc ctgccgcgct cgtgctcaca ctttgctcgc ggctcgttgc cccgctcagc
     1261 acgctgtaca ccaacgactt cataagcgct gaattctctc ttgacctgaa gatggcggtc
     1321 ggcgtgacgg ggccgctcgc cgtactgctg ctactcagct tgctgcccag gtttcggagg
     1381 gaagcgaagg agtgatcggc gctttgggaa gtccgcttcc ggagcacgtg ctcgacatgg
     1441 cgctggtgta gcatgtgcgc tgagatagct cccctgggat tggaacaagc gtggaactcg
     1501 actggctgaa ggactgggtt cagatcgcgc agggtctggc gaccatagca gcgatcttga
     1561 tcgcaggcat ctgggcttac tacgtgttcg ccgtcgggcg cagtgccacg gcccaggtgc
     1621 agatcacatg ccacctcaaa gaggtgatcg catcaccagg cggaaagatc ctgtgtatct
     1681 cggtaatgct tactaacacg ggccggaccc gcgtggccga cctgtcgtgt gagattatcg
     1741 ctgcgcctgt tgccaattca ggagacgatc gagcagctct tgctttactg gagccactac
     1801 cagaacgcat tttggacgaa ggtgaacccg gctctgattt cgcatggggc aagatgttta
     1861 gagagacggt gggactggaa cccaacgagg cggtagctga agatattctg ctatcggtac
     1921 gggccggatc tcccgcgtca gcggacgatc ccggcgcgtt cgcggcagag gtgcattgcc
     1981 ggggcaatat cttgggaggc cgcaaaggtg attggggttg gcgcggcatc ctggattcac
     2041 gaaaggttgg agaataagtc gcaacctcga tgacattgac cgggaagagc cacgagtgta
     2101 gtctgcgata gagggctgat gggggataac tgatatcgaa gaatacaatc cgcgacggaa
     2161 cctgaggagc ttgatcttcg agatagtgga ggtgctcggg cgatctggcg acatcgacga
     2221 gcaaactcgc accgcgatcc tcgatggtgt gcacgagctt aggcgcgaca tgcttggtgc
     2281 cagcgacgag aagcttgaag agtggagtac gagcgtggag cggtgtatga aaatgtcgag
     2341 cgtcgagttt ggatcggggc tcagccaggc ttccgctgag gaccttcgcg ggtgtctaga
     2401 cgggatcagg cactagagga actcttcggc ctcggcgagt tggccggtct tatcgaaggc
     2461 cctgcgcagt aggagtcgtc cctcttcgcg ctcctcctcg gtgcgggcct tctcgatcac
     2521 ggtctttact gactccctga tggcgtctgg ctcgaacgtg atatcgggca gcgcgatgac
     2581 gacctcacgg atctggcggt atatgaagtc aggctcgccg gcgtataccc acccgcggcg
     2641 ggcgagtacc caggcccagc ctttcatgcg gatggtgttc tctatgtcct cgtcctcgag
     2701 gccgaacatc tcgatcctga gcctgtgggc ggttatgacc tccggtctaa tgcgtaccgt
     2761 cgttggcatg tggacagttt agcagcgacg agtcggctgg ctgttagagg attcgccatg
     2821 actttatctt cgccgcgaac ctgtccccgc cgaggtggta ggcgacgtga cgccagtctg
     2881 gggcgtcgag gaggagcaca tcggccatgt agcccggtgc gagcctgcca acgtcttcga
     2941 ggccgaggac gtgcgcggcg ttcgccgtac aggcgcagag ggcttccgcc ggggagagcc
     3001 cgaggcgggt gcacgccagg ttcatcacca gcggcagcga ctcgcagaag gagcttccgg
     3061 ggttgaagtc ggtggcgagc gccaggatcg ctccctcgtc ggccaggagg cgcgccggag
     3121 ggagcggcag gtcgaggaag a
//
LOCUS       DATAIR010000088         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_374149, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000088 DATAIR010000000
VERSION     DATAIR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374149"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /locus_tag="VHM69_02245"
     CDS             complement(<1..2501)
                     /locus_tag="VHM69_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2739240.1"
                     /translation="MVEVEVGVSVISYAGMDVICAVLRDITERKRVEEALRSSESRLA
                     AAQRLVHVGSWEHLLEEDRLHWSDELYRIFGFAPGAFDPTYETLMERIHPEDRGLLQR
                     TVREAVRDRKQPALVCRIVRPDGEVRVVQFQYEIEYGEGGAAVRMSGALQDITERKRT
                     EEALRDAEARFRTLVEQIPATTYVEEPSGKGKTLTYMSPQYEAMLGYTPEEGMSHPEH
                     WLELMHPEDRERVLAEDRRTDETGEPFAAEYRMFAAEGSVVWIRDEALLVRDDKDRPL
                     FWQGVMLDITGRKEAETKLREAEERYRTLVERMPAVTYIDRADDSDEPLYTSPQIEEL
                     LGYTQEEWLRGRLWIEHLHPEDRERVMSANERFEAGGEFFGEEYRIIARDGSVVWVRE
                     EAVLVSDERGGRRFRQGFILDITGRKEAEEALRSSEARFRSVVESVGEGLLITDDRDV
                     ILYVNSRMTELTGYSEEEMLDHPAYELFLPPEEWPDMMERNKRRTAGSPERYEMRLKR
                     RDGETFWAEISATPYTNASGEVVGTLGALTDITERKRTERSLEESEQRFRQLFEQSVD
                     ALFVHDERGRFVDCNSQATLLLGYSREELLTMSVWDVSEDMLTPEEKARKARDGGTLW
                     QRALSGAPGTFALSHEEMNRRKDGTKFPVEVRVGSVDYGGSRMLLTSVRDITKRKLSE
                     EDLRKSEGRHRRQARELSLLYRVSTALAQQFDLSAIFSTVVEAVAESFGYTQVSAYQL
                     EDGVLTLQHQVGYHEVIDRIPTSAGVAGRVASSGRPVLLEDVREDPSFLGAIDDITSE
                     VCVPLFDQDTVVGILNLESVGGVRLTRADLRLTSAL"
BASE COUNT          412 a          872 c          752 g          512 t
ORIGIN      
        1 agggcgctcg tcagccgcag gtcggcccgc gtgagcctca cgccgcccac gctctccaag
       61 ttgaggatgc cgacgaccgt gtcctggtcg aacagaggaa cgcacacttc cgaggttatg
      121 tcgtctatcg cgccaaggaa agaagggtct tcccgcacgt cttcgagcag aacgggtcgg
      181 cccgaggatg ccacccgccc tgcgacaccg gcggaggttg gtatgcgatc gatcacctcg
      241 tggtagccaa cctgatgctg gagcgtcagc accccgtctt cgagctggta ggcgctgacc
      301 tgggtatacc cgaagctctc agccacggcc tcgacgacgg tgctgaagat ggccgagagg
      361 tcaaactgct gcgccagcgc cgtgctcacc ctgtagagta gggagagctc ccgcgcctgg
      421 cggcggtggc gcccctcgct cttcctgaga tcctcctcgg agagcttgcg cttggttatg
      481 tcgcgaacgg aggttagcag catcctactt ccgccgtaat cgaccgagcc tacccgcacc
      541 tccacgggga acttggtgcc atccttgcgg cggttcatct cctcgtggct gagcgcgaag
      601 gtgcctggcg cgccgctcag ggcgcgttgc cagagcgtgc cgccatctct cgccttcctg
      661 gccttttcct cgggggtgag catgtcctcc gagacgtccc acacggacat agtgagcaac
      721 tcctcgcggg agtagccgag aaggagggtc gcctgcgagt tgcagtcgac gaaacggccc
      781 cgctcgtcgt ggacgaagag ggcgtcgacg gactgctcga agagctgcct gaagcgttgc
      841 tcgctctcct cgagagaccg ttctgtccgc ttgcgctccg tgatgtcggt gagcgcacct
      901 agcgtaccga cgacctcgcc ggatgcgttc gtgtaagggg tcgcgcttat ctcggcccag
      961 aatgtctccc cgtccctgcg cttgagacgc atctcgtagc gctcggggga ccctgccgtc
     1021 ctccttttgt tgcgctccat catgtcgggc cactcctcgg gcggcaggaa gagctcgtac
     1081 gcagggtggt cgagcatctc ttcctcggag tagccggtca gctccgtcat gcgtgagttc
     1141 acatagagga tcacgtcgcg gtcgtcagtg atcagtaatc cttcgcccac gctctcgacg
     1201 accgagcgga atcgcgcctc ggagctgcgc agcgcctcct ctgcctcttt gcggcccgta
     1261 atgtcgagga tgaagccctg cctgaagcgg cgtccacctc tctcatcgct tacaagcaca
     1321 gcctcctcac gcacccacac cacagacccg tccctggcga tgatgcggta ctcctcgcca
     1381 aagaactcgc ctccggcttc gaagcgctcg ttggccgaca taacgcgctc tctgtcctca
     1441 gggtggaggt gctcgatcca gagtcgtccc cgcagccact cctcctgggt gtagccgagc
     1501 aactcctcta tctgtgggct tgtgtagagt ggttcatcgg agtcgtccgc ccggtctatg
     1561 taggttacgg caggcatccg ctcgacgagg gtgcggtaac gttcctccgc ctccctgagc
     1621 ttcgtctctg cctccttacg gcccgtgata tcgagcatga cgccctgcca gaacagaggc
     1681 ctgtccttat cgtcgcgaac cagtaaggcc tcgtccctga tccagaccac agatccctcg
     1741 gccgcgaaca tcctgtactc cgcagcgaac ggttcgcccg tctcgtccgt gcgcctgtcc
     1801 tccgcgagta cccgctcgcg gtcctcggga tgcatcagtt cgagccagtg ctccggatgg
     1861 gacataccct cttcgggcgt gtagccgagc atcgcctcgt actgcgggct catgtacgtg
     1921 agcgtcttgc ctttaccgga aggctcctcg acataggtgg tcgctggaat ctgctccacg
     1981 agcgtccgga agcgagcctc ggcgtcacgc agcgcctcct ccgtccgctt gcgctccgtg
     2041 atgtcctgga gcgcaccgga catccgcacc gccgcgcctc cttcgccgta ctcgatctcg
     2101 tactggaact ggacgacgcg cacttcaccg tcaggcctga cgatgcggca cacgagggcc
     2161 ggttgtttcc tgtcgcggac cgcttcacgg accgttctct ggaggaggcc gcgatcttct
     2221 ggatggatgc gctccatgag ggtctcgtag gtggggtcga acgcgccagg cgcgaagccg
     2281 aagatgcggt agagctcgtc cgaccagtga agccggtctt cctccaagag atgttcccag
     2341 ctgccgacgt gcacgagccg ctgggcggcg gcgagacggc tttcgctgct ccgcagagcc
     2401 tcctctaccc gtttgcgctc ggtgatgtca cgcagaacag cacagatgac atccatccct
     2461 gcgtaggaga tcacactcac ccctacctct acctctacca ccttcccatc cttgcgtcga
     2521 tacctcctct cccctacata gcgtgccc
//
LOCUS       DATAIR010000089         5940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_97556, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000089 DATAIR010000000
VERSION     DATAIR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97556"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..573)
                     /locus_tag="VHM69_02250"
     CDS             complement(205..573)
                     /locus_tag="VHM69_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739241.1"
                     /translation="MESGRITRQWLEGLDPAEFQEISYMISDIQRERASARRRKLERR
                     RAAARKKAQNKRYLKLERVNCGKKGCKKCASGELHGPYWYLYRPKVNAPGRTSEYVGK
                     LLPETLAEEFGIPEVDRSAS"
     gene            complement(678..1100)
                     /locus_tag="VHM69_02255"
     CDS             complement(678..1100)
                     /locus_tag="VHM69_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2739242.1"
                     /translation="MDELRVPNLEEMDEQQAHEFLSELFSNLSAEAEYELRHEDYTME
                     MPQSGERIRGRDKMREFQEAYPTPPTIQLRRVVVSEGLWILEGVNDYGEGQIFNVVVI
                     IELKDGKMWRDTRYYAQPFNAPEWRAQWVERMEFSDTS"
     gene            complement(1286..1999)
                     /locus_tag="VHM69_02260"
     CDS             complement(1286..1999)
                     /locus_tag="VHM69_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX2739243.1"
                     /translation="MSETNLEQFREVTPVGRFTLQNSAMLKVRLENDQIQAKLGSMVA
                     YQGDVRFEHKSGGLGRFFKKAVTGEGVKLMVATGTGDLFLAQDKRKIMILDLENERMT
                     VNGDNILAFEPGIDWDIKRVEGAGRLAGGLFNVILEGTGKVAVSSDGEPVLLDTSTPT
                     FADPDSAIAWSGGVRTSVRSDVSFKTFTGRGSGESFQIAFQGAGWVLIQPSEGPVIPE
                     HSHAGQGSSSGGLGSLFED"
     gene            2213..3115
                     /locus_tag="VHM69_02265"
     CDS             2213..3115
                     /locus_tag="VHM69_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2739244.1"
                     /translation="MRKTILLGASMASALVLACVAASLTAVPGVGQTATATLVGAGDI
                     ASCNYNEDSATARLLGRIPGTVFTLGDNAYTYGTAADFKNCYDPTWGKYRKRTRPTAG
                     NHDYYTAGAKPYFDYFGWRAGRPSRGYYSYDRGAWHIVALNSNCNKVGGCGRRSEQGR
                     WLRKNLANHQARCTLAYFHHPLYATGNGTATAQVKPFWNILYDRGADVILSGHAHRYE
                     RYAPIRPGGATDQANGIRQFIVGTGGNSGGGDIHYNTAPGVQVVKLRTPGVLKLDLGA
                     GSYTWKFVPIAGKTWSDSGTDQCH"
     gene            complement(3267..3605)
                     /locus_tag="VHM69_02270"
     CDS             complement(3267..3605)
                     /locus_tag="VHM69_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX2739245.1"
                     /translation="MPEISWEDFEKVNIRVGKVVEAETFPEARKPSIKLVVDFGPQLG
                     IRRTSAQLTAHYDPQMLVGKQVVAVVNFPPKRIAGFKSEVLVLGVPDENGGVVLLSPD
                     HEVPPGGRMF"
     gene            complement(3670..4494)
                     /locus_tag="VHM69_02275"
     CDS             complement(3670..4494)
                     /locus_tag="VHM69_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2739246.1"
                     /translation="MYPGDQTREKEPEIGLDREHSEPLKGVPLYLQVERRIEDLLLGG
                     RYREGDRIPPEAELVDTLEVSRVTVRAGLARLVERGVLERRQGSGTFLVRPPGGSRLA
                     AGLERLETYTVHAKRLGLKLDSRDLSIVAGGAASDEAAALEVPGGTSLVKVSRVLLIE
                     GKPAAWMVDVVPEDVIGFEELRERFRPDAMVLDVLVSEGVPVRFSQMFIEAVVLDPDD
                     QIGRTLGLKALSAALSLTQTMYLVDGRPVQWSQDTFLPGHLNLHVVRELFEVRNLS"
     gene            4688..>5940
                     /gene="eat"
                     /locus_tag="VHM69_02280"
     CDS             4688..>5940
                     /gene="eat"
                     /locus_tag="VHM69_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEX2739247.1"
                     /translation="MSETERRAGQGAEYENVDTEYMEQRQLQSGAAGWVLLAGLGVAY
                     VISGDFAGWNFGLAEGGWGGLLIATLLMATMYTAMVFSLAELSSTIPTAGGGYGFARR
                     AMGPWGGFLTGTAILIEYAIAPAAIATFIGGYVESLIGIGGWIVYLAVYLIFIGIHLY
                     GVGEALKLMFAITAIAVVALAAFVIGMIPQFEPSNLFNIDPTNAAGASSFLPFGVVGV
                     WAALPYAIWFFLAIEGVPLAAEESRDPTRDMPRGLIAGMMALLVFAALILVFGPGAAG
                     SAAIQDSANPLPLAVEKAYGGGNALSTFVNIAGLAGLIASFFSIIFAYSRQTFALSRA
                     GYLPRLLSVTGSRRTPWAALAVPGLIGFLLTLLPPEVTGNGAILINIAVFGATISYVL
                     MMLSHIVLRRREPDLERPYRTPGGVL"
BASE COUNT         1082 a         1876 c         1751 g         1231 t
ORIGIN      
        1 ccagggtccg tccgcatatc gaaggcaagg aactccgtaa gagcgtctac gtgccaggaa
       61 gactcgtcaa cctcgtcgtc ggttagagca gggttctcca acgggagtag acggggaacg
      121 gaccgtatcg ccgcagcctg cgtcgtcatc ttatgtacag gcaacccgca tactctgcgt
      181 gtgcgtacat aagaatcaaa tttgctagct cgctgaccga tctacttcag ggatgccgaa
      241 ctcttcggcc agtgtctcag gcagcagctt accgacgtac tcgctcgtac ggccgggagc
      301 gttcaccttc gggcggtaga gataccagta aggaccgtgg agttctccgc tggcgcactt
      361 cttacagcct ttcttgccgc aattgacgcg ttccagtttg aggtagcgct tattttgggc
      421 tttcttgcgg gctgcggccc ttcgccgttc gagtttacgt cgtcgcgcgg acgcgcgctc
      481 acgttgtatg tcggagatca tgtacgagat ctcctggaac tccgctggat ccaagccctc
      541 caaccattgc ctggttatcc gccctgattc catgacgccc ttcatattag gtctcttatg
      601 tacatcctac cacggagtct acttcagtcc atacataaga gccccgtatg actgctgatt
      661 tcccgatgat cttcaggcta gctggtgtct gagaattcca tccgctcgac ccactgagcc
      721 ctccactcgg gcgcgttgaa cggctgcgcg tagtagcggg tatcccgcca catcttcccg
      781 tccttgagct cgataatgac cacgacgtta aatatctgcc cctcaccgta gtcgttgacc
      841 ccctctaata tccacaaccc ctcactgacg accacccggc ggagctgaat agtaggggga
      901 gtaggataag cctcctggaa ttctctcatc ttgtctctgc cccggatacg ctcgccggac
      961 tggggcatct ccattgtgta gtcctcgtgg cgtagctcgt attccgcttc cgcgctcagg
     1021 ttggaaaaca gctcggagag gaactcgtgc gcctgttgct catccatctc ttcgagattg
     1081 ggcaccctta gttcgtccat ctgcgcttct ccttccgccg ggctaccaac ccgcaccgcg
     1141 gtgctggatc agcgaagttc agtgcatctg acggcctctt tgtgcaagca gacctccggg
     1201 acctgcccga gcaacgcgta ctacagggga caatgaggac cgcgttttcg gccctcagta
     1261 gtccgcagtt ggactgaact tccgcctagt cctcgaacag cgagcccagg ccgccgctgc
     1321 tgctgccctg accggcgtgg gagtgttcgg ggatgacggg gccttccgaa ggttgtatga
     1381 ggacccagcc tgcaccctgg aacgctatct ggaacgactc tccgctgccg cgtcccgtga
     1441 aggtcttgaa ggaaacgtcg ctcctgacgc tagtcctcac gccgccgctc catgctatcg
     1501 ccgagtcagg gtcagcgaag gttggtgtcg aggtgtctag aaggacgggc tccccgtccg
     1561 agctcacggc gaccttgccc gttccctcca ggatcacgtt gaagaggccg cccgcgaggc
     1621 ggcccgcgcc ctcgacccgc ttaatgtccc agtcgatgcc tggctcgaag gcgaggatgt
     1681 tgtcgccgtt cacggtcatg cgttcgttct cgaggtcaag gatcatgatc ttgcgcttgt
     1741 cctgagcgag gaacaggtcg cctgttcctg tggcgaccat gagcttgacg ccctcgcctg
     1801 tgacggcctt cttgaagaag cgtcccaggc ctccgctctt atgctcgaaa cgaacgtcgc
     1861 cctggtaggc gaccatcgat cctagcttgg cctggatctg gtcgttctcc aggcgcacct
     1921 tgagcatcgc cgagttctgc agcgtgaatc gtcctacagg ggttacttcc ctgaactgct
     1981 cgagattcgt ctcgctcacc accgtacctc cctgactagt tttgtaacca tgatctacta
     2041 cgtttcgtcc gccgtccagg tttctgagcg cccggtcctt cgcgggccac aaaattgtcc
     2101 gaatacagtc cactcgggcg tagcgccgac ttcgagtcga gggtatgatc caccggtcac
     2161 gcaacggggc ggggaacaca cctttctctg cccgattgat gaagggatgt gaatgaggaa
     2221 gacaatcctt ctgggcgctt cgatggcatc agcactggtg ctggcgtgcg tggcggcctc
     2281 tctgacggcg gtgcccggcg tcggtcagac ggcgacggcc acgctggtcg gggcgggaga
     2341 cattgccagc tgcaactaca acgaggacag cgcaacggcg aggcttctgg gcagaatacc
     2401 ggggacggtc tttaccctcg gggacaacgc ctacacctac ggcaccgcgg cggatttcaa
     2461 gaactgctac gatcccacct ggggcaagta caggaagcgc accagaccca cggccggcaa
     2521 ccacgactat tacaccgccg gggcgaaacc ctacttcgac tacttcggat ggcgggctgg
     2581 caggcccagc cggggctact acagctacga tcgaggagcc tggcacatcg tcgccctcaa
     2641 cagcaactgt aacaaggtgg ggggctgcgg caggcgttct gaacagggac gctggctaag
     2701 gaaaaatctc gccaaccacc aggcgagatg taccctggcc tacttccacc acccgctgta
     2761 cgccaccgga aacggtaccg caaccgccca ggtgaaaccc ttctggaaca tcctctacga
     2821 ccggggcgcc gacgtaatac tctcggggca cgcccaccgc tacgagcgct acgccccgat
     2881 aagaccaggc ggcgcgacag accaggccaa cggtatccgc cagttcatcg tcggtaccgg
     2941 gggcaactcg ggtggcggtg atatccacta caacactgcc cctggagtgc aggtcgtgaa
     3001 gctcaggacg cccggtgtcc tcaagctaga cctcggcgcc ggctcttaca catggaaatt
     3061 cgtacccata gcgggcaaga cgtggagcga ctcgggcacc gaccagtgcc actaaggcga
     3121 tttgcagaag tgccttctgc aaatcgcgca atcagcagtc aggcatcagc tttcagcttt
     3181 ttgttctagc tgaacgctga ttgctgacag cgtttgcaaa gagggattca acctctttgc
     3241 aaactcgctt taggaggttg tcttcctcag aacatcctgc cgccaggcgg gacctcgtgg
     3301 tctggggaca ggagcacgac tcctccgttc tcgtcaggga cgccgaggac gaggacctcg
     3361 ctcttgaagc ccgcgatcct cttcggtggg aagttgacca ccgcaacgac ctgctttccg
     3421 acaagcatct gcgggtcgta gtgggcggtg agctgcgcgc tggtcctcct gatcccgagc
     3481 tgcggaccaa agtccacgac gagcttgatc gagggcttgc gggcctctgg gaaagtctcg
     3541 gcctcgacga ccttgccgac cctgatgttc accttctcga agtcttccca gctgatctcg
     3601 ggcaattctg gctcctttgt cgggggctac aagttcaacg catcaacgaa tcgttgcata
     3661 cctgatgcct tatgaaagat ttcgtacctc gaatagctct ctgacgacgt ggaggttaag
     3721 gtgtcctggg aggaaggtgt cttgcgacca ctgcacagga cgaccgtcga cgaggtacat
     3781 ggtctgggtc aacgagagcg cggccgagag agctttcagg cccagggttc tcccgatttg
     3841 gtcgtccgga tccagcacga cagcctcgat gaacatctgg gaaaagcgca cgggaacgcc
     3901 ctctgagacc aggacatcga gcaccatggc gtcgggacgg aagcgctccc gaagttcctc
     3961 gaacccgatg acgtcctcgg gaaccacgtc caccatccac gccgcgggtt ttccctcaat
     4021 gagcagaacg cgcgatactt tcacgagcga ggttcctccg ggcacctcca gcgccgccgc
     4081 ctcatctgaa gcggccccgc ccgctactat gctcaggtcc ctgctgtcga gcttgagacc
     4141 aagccgctta gcgtgaaccg tgtacgtttc cagccgttcc agacccgccg cgagccggct
     4201 tccccctggt ggtcgaacca ggaacgtacc gcttccctgg cgtcgctcga gtacccctcg
     4261 ctccacgagc cgcgccagtc cagcccgcac cgtgacccgc gaaacctcga gggtgtcgac
     4321 cagctcggcc tcgggcggta tcctgtcccc ctccctgtag cggcccccca gcaggaggtc
     4381 ctctatccgc cgctcgacct ggaggtagag cgggacgccc ttcagcggct cagaatgttc
     4441 tcgatccagc ccgatctcag gctccttctc tcgcgtttga tctcctgggt acaccagatg
     4501 ctacccgaca actcctgccc cgcaagagaa atacatgctc tgtctacttg tgtgacaaat
     4561 tggtattact tatgatgcga ttgtagccag cgcgctaacg cgagagttgg gcattgtctt
     4621 gagacggcgg gacgaggagt accgtcgcgg gttctgtagt tggcgatttg gggaaaggga
     4681 ggttgggatg agtgagaccg agaggcgtgc gggccagggg gccgagtacg aaaacgtaga
     4741 caccgagtac atggagcagc gccagctgca gagtggagcc gccggctggg tgctccttgc
     4801 tggcctcggg gtggcatacg tcatctccgg cgacttcgcg ggctggaact tcgggctcgc
     4861 cgaaggtggt tggggagggt tgctcatcgc caccctgctc atggccacga tgtacacggc
     4921 gatggtcttc tcgctggccg agctctcgtc aaccataccg accgccggcg gcggctacgg
     4981 gttcgccagg cgggcgatgg ggccgtgggg cgggttcttg acggggacgg cgatcctgat
     5041 cgagtacgcc atagcaccgg cggccatcgc tacgtttatc gggggatacg ttgagtcact
     5101 catagggata gggggttgga tcgtctacct ggccgtatac ctgatcttca ttggcatcca
     5161 tctctacggg gtcggcgagg cgctcaagct gatgttcgcc ataacggcca tcgccgtcgt
     5221 cgcgctcgcg gccttcgtca tcgggatgat cccgcagttc gagccttcca accttttcaa
     5281 catcgacccg acgaacgcgg ccggggccag ttcgttccta cctttcgggg tggtcggcgt
     5341 ctgggcggcg ctgccttatg cgatctggtt cttcctcgcg atagagggcg tgccgctcgc
     5401 cgccgaggag agccgcgacc ccacgcgtga catgccgagg gggctgatcg ccggcatgat
     5461 ggcgttgctt gttttcgcgg cgctcatcct cgtcttcgga ccaggcgcgg cgggctcggc
     5521 ggctatacaa gattccgcca atccgttgcc gcttgctgtg gagaaggctt atggcggcgg
     5581 caacgcgctc agtaccttcg tcaacatcgc gggcctcgcg ggcctgatcg cgagcttctt
     5641 ctcgatcatc ttcgcctact cgcgccagac cttcgcgctc tcgagggccg gttatttgcc
     5701 gagactgctc tcggtcacgg gtagccggag aacgccgtgg gcagcgctcg cggtgcccgg
     5761 cctcatcggc tttctcctga ctctccttcc gcccgaagtc acggggaacg gggcgatcct
     5821 gatcaacatc gcggtcttcg gagcgacgat ctcctacgtc ctgatgatgc tctcgcacat
     5881 cgtcttgagg cggcgtgagc cggatctcga acgcccatat cgcacacccg gcggcgtcct
//
LOCUS       DATAIR010000090         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_282532, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000090 DATAIR010000000
VERSION     DATAIR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282532"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM69_02285"
     CDS             <1..318
                     /locus_tag="VHM69_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2739248.1"
                     /translation="SLANPRGGVAHGWEQVSETIERAAASRRDGETVGFEIMAKYVTP
                     ELACVVQVERLKARFDGGEDITQYALRVTMIFRPENGVWKVVHRHADSLTTVQPAASY
                     EVG"
     gene            725..>1999
                     /locus_tag="VHM69_02290"
     CDS             725..>1999
                     /locus_tag="VHM69_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX2739249.1"
                     /translation="MHEIAFQIEFLIGLLGVVALLAQLARMVGVPYPIFLVLGGIGVG
                     LVPGLPQIELPPEIIFLVFIPPLLLSAALSSSPRDLRAHARPIGLLAVGLVLLTMTAV
                     ATVAHFVADLPWAAAFVLGAVVAPTDPVAAEATFRRLGVPDRVQTIVGGESIINDGTA
                     LVAYNIAVAAVVTGSFSVAGAGLEFLLVSGGGIILGAVVARLVTPLYRRLTDPTILVV
                     FTLIPAYFSYIVAERLGVSGILAVVTLGLYVGWQAPKLFTARARVQSYSFWEVLTFLM
                     DSLLFILVGLQFPVILDGMSEYTFAHLLLYAALVCGVVIGLRLLWFFLAPSSIPALDR
                     LLRTRYLRAPLKERFVMGWSGMRGAVSLAAALAVPLQTHQGVDFPQRDLIIFLTYCVI
                     FATLVLQGLSLPFLILSLGVRDEGHDTRMAELN"
BASE COUNT          308 a          662 c          622 g          407 t
ORIGIN      
        1 agcctcgcca atccccgtgg cggggtcgcg catggatggg agcaggtttc ggagaccata
       61 gagcgtgccg cggcgtctcg ccgagacggc gagaccgttg gcttcgagat catggcgaag
      121 tatgtaactc cggagcttgc ctgcgttgta caggtagagc ggctgaaggc caggttcgat
      181 ggaggcgaag acatcaccca gtacgccctg cgggtcacga tgatctttcg acccgaaaac
      241 ggtgtgtgga aggtcgtgca tcgccacgcg gactcgctaa ccacggttca acccgcagcc
      301 tcctacgagg ttgggtgaat acacctccgg cgagtcacgt tggcggagat ggacaatccg
      361 ccgccggctg aaggtgcccg atcaggcctc cgcgatgatc gacggttctc acgccatcat
      421 acttccggcg tagttaccgc aacctccgcg aggcccgcga atgacaccag cagaggttgc
      481 gtcctttccg acctgtgatc atggacgctg gagggcagag aggccatgta gaatactgat
      541 gcggtacgca agccggcgac gtgatcgtag aggttttcga ccgtgacgac gcggtaacgt
      601 tcggtggtgg agctctcgcc accacgcaga cggctaccac ctcggcgaga tcgtcatggg
      661 gtcttgtgat cagagaggag agagcagcta ttgtcgtaga acgccgcagg ttttcagggc
      721 gcccgtgcac gagatagcgt ttcaaatcga gttccttata ggtctccttg gcgtcgttgc
      781 cctgctcgcg caactggccc gcatggtcgg cgttccttac ccgatcttcc ttgtcctcgg
      841 cggcataggc gtggggctgg tgcccggtct cccgcagata gagctcccgc ccgagatcat
      901 cttcctcgtc ttcatcccgc cgctgctgct atcggccgcg ctctcgtcct ccccgcgcga
      961 cctgcgcgcc cacgcccgcc cgatagggct tctcgccgtc ggcctcgtgc tcctgacgat
     1021 gaccgcagtc gccaccgtcg cccacttcgt cgcggacctg ccgtgggccg ccgccttcgt
     1081 cctcggggcc gtcgtcgcgc ccaccgaccc cgtggctgcc gaagcgacct tccgaaggct
     1141 cggcgtcccc gaccgcgtgc agaccatcgt aggcggcgag agcatcatca acgacgggac
     1201 ggcgctcgtg gcatacaaca tcgccgtcgc tgctgtagtc acgggctcgt tctccgtggc
     1261 gggagccgga ctggagttcc tcctcgtgag cgggggcggc atcatcctcg gggccgtcgt
     1321 ggccaggctc gttacgccgc tatacaggcg gctgaccgac cccacgatcc ttgttgtatt
     1381 caccctgatc cccgcctact tctcctacat cgtcgccgag agattgggcg tctccggcat
     1441 cctcgccgtc gtcaccctcg ggctgtacgt ggggtggcag gcacccaagc tcttcaccgc
     1501 cagggcgcgc gtgcagagct actccttctg ggaggtcctc accttcctta tggactccct
     1561 gctgttcatc ctcgtcgggt tgcagttccc cgtgatcctg gacgggatga gcgagtatac
     1621 gttcgcgcac ctcctccttt acgccgcgct cgtgtgcggc gtggtgatag gtctgcggct
     1681 gctgtggttc tttctcgcgc cgagctccat cccggccctg gaccgtctgc tccggacgag
     1741 gtacctgcgg gcgcccctga aggagcggtt cgtgatgggc tggagcggga tgcgcggggc
     1801 ggtatccctg gctgccgcgc ttgccgtgcc tctgcagact caccaaggtg tggactttcc
     1861 ccagagagac ctcatcatct tcctcaccta ctgcgtcatc ttcgccacgc tggtactgca
     1921 gggactcagc ctgccgttct tgatcctctc tctgggcgtg agggacgagg gacacgacac
     1981 ccgcatggcc gagctgaat
//
LOCUS       DATAIR010000091         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_48569, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000091 DATAIR010000000
VERSION     DATAIR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48569"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHM69_02295"
     CDS             <1..592
                     /locus_tag="VHM69_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739250.1"
                     /translation="GGGLMEGRRERIVGGLAALSLVPGGLIAYMGYLWIRFGDPLLFY
                     SAQKNWGRQATGPLATAGRAWESAAEGAGRLIDPGLWAHPTLGNLADHLATANNLYNL
                     AFFVFALVVLLAGLRELPLSLSIYGLLLVAPATLFGTPESPLMGTPRYVLVSFPIFIV
                     LGLLCGRKLLFGGWLLLSTVLSLVLCGLFVSWRFVA"
     gene            complement(611..997)
                     /locus_tag="VHM69_02300"
     CDS             complement(611..997)
                     /locus_tag="VHM69_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739251.1"
                     /translation="MRQSGLLVAVVGLLLLSLAAGDALAGAELGGRGDDALRGSGRDD
                     RLTGFGGEDGIWGFAGDDGLSGGPGHDELYGGPGRDTLLGGTGDDFVETKDGERDYVD
                     CGPGKDAASVDMQDRVSHTCETLYAA"
     gene            1276..1974
                     /locus_tag="VHM69_02305"
     CDS             1276..1974
                     /locus_tag="VHM69_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739252.1"
                     /translation="MAEREFRLPGSSYEEIVNIIVAYGTRDEAARAGDVGKLDSVHQS
                     SVSRNNAFLTEIGVLQGESKKLITRRGRTLAVALARKDRAEIRSNWRTIVAASEFLQN
                     VVSAVKLREGMLYPTVQAYIAHAAGQPRNKPVMNGAGAIIEILKAAGLLKEEAGELVA
                     TFDEQPEDPASENGSPVEPSGTSVVSATVSGAVPAGTSPTLSIHVQVRCTADEIEDLA
                     PKLKALLRELSAED"
     gene            complement(1949..2260)
                     /locus_tag="VHM69_02310"
     CDS             complement(1949..2260)
                     /locus_tag="VHM69_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739253.1"
                     /translation="MANELTSYRQTIAFVISEIHPNVEVFEADPETLDREVARLRPDL
                     VICSRITRLVRDMVPNWVELYPDCESFSTLCIGGERSAMEDVQLDDLLSLCASPLQRA
                     P"
     gene            complement(2353..>2725)
                     /locus_tag="VHM69_02315"
     CDS             complement(2353..>2725)
                     /locus_tag="VHM69_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739254.1"
                     /translation="PAAGAASAGHRHGRPLVLMANEPRAYREGIAAVIGQLRPEVEVT
                     TVEPNALDASIIRFSPDMVICSKATDALKGRVPVWVELYPENAARSVASIGGRRMEYA
                     EIQLPDLLSIVDQAEELAGAN"
BASE COUNT          501 a          862 c          853 g          509 t
ORIGIN      
        1 gggagggggg ctaatggaag ggaggaggga gaggatagta ggggggttgg cggcactttc
       61 attggtaccg ggagggctga ttgcatacat ggggtatcta tggataaggt ttggagatcc
      121 cctcctcttc tactcagcgc aaaagaactg gggaaggcag gccaccgggc cgctcgctac
      181 cgcgggcagg gcgtgggagt cggccgccga gggcgccggc aggctcatcg accccgggct
      241 atgggcgcac cccactctgg ggaaccttgc agaccacctc gcgacggcga acaacctcta
      301 caacctggcg ttcttcgtat tcgccttggt cgtgctgctc gccgggctgc gagagttgcc
      361 cctctccctg agtatctacg gcttgctgct cgtcgccccg gccacgctct tcgggacgcc
      421 tgagagcccg ctgatgggca ccccgcgata cgtgctcgtc tcattcccca tctttatcgt
      481 gcttgggctc ctttgcgggc gaaagctcct cttcggcgga tggctactcc tgagcaccgt
      541 cttatccctg gtcctgtgcg ggctcttcgt gagctggcgg ttcgtcgcct gaacattggc
      601 tcgcgctgga tcacgccgcg taaagcgtct cgcaggtgtg cgagaccctg tcctgcatgt
      661 ccacgctggc agcgtctttg cctgggccgc agtccacata gtcccgctcg ccgtccttgg
      721 tctcgacgaa gtcatcccct gtaccgccaa gcagcgtgtc gcggccgggg ccgccgtaga
      781 gttcgtcatg gccgggtccg ccggagagac cgtcgtcgcc cgcgaagccc cagataccat
      841 cctcaccacc gaagcctgtc agcctgtcgt cccggcctga gccgcgcaac gcgtcgtcac
      901 cacgtccgcc caactcggca cccgcgagcg cgtcgccggc ggccagggag agcaacaaca
      961 gcccgaccac ggctaccagg agtcccgatt gccgcaagcc tttccccgcg gcccctggat
     1021 ctctgccgta tcgaagcggg atcgccggtc gaaccggctt cctcaggttc acacgacccg
     1081 tatccagtgt ctgcgcgctg ctgaaggccg tcggttcata caaagaaatt ctaggaacag
     1141 gcgggcgacc tgtggttaaa cgagggaaac ttccagctta aatcttcgtt aactaccctg
     1201 gcgccgaggg ctggcatctc cacgtcgcgg cgtccactat aatggcctga gcgcttgcga
     1261 taagagaggt acaggatggc ggagagggag tttcgacttc cgggatcctc ttacgaggag
     1321 atcgtcaata tcatcgttgc ttacgggacc cgcgacgagg cggcgagggc cggcgacgtt
     1381 ggcaagctcg actccgtgca ccagtccagc gtcagccgca acaacgcctt cctaacggag
     1441 atcggggtgc tacaaggtga gagcaagaag ctcatcacac gccgcggacg cactctggcc
     1501 gtggcgctcg cccgcaagga tcgggccgag atccggagca actggcgcac tatagtcgcc
     1561 gccagcgagt tcttgcagaa cgtggtctca gccgtgaagc tgcgcgaggg gatgctctac
     1621 cccaccgtcc aggcctacat agcgcacgcc gcgggccagc cgcgcaacaa gcctgtgatg
     1681 aacggcgccg gcgccatcat cgagatactg aaggccgcgg gtttgctgaa agaggaggcc
     1741 ggagagctgg tcgccacctt cgacgaacaa cctgaagacc ccgcctcaga gaatggttcg
     1801 cccgtggaac cttcagggac gtccgtggtc tcagcgaccg tgagcggagc cgtacctgcg
     1861 ggcacaagcc cgacgctaag catccacgtc caggtgaggt gcacggctga tgagatcgag
     1921 gacctcgccc cgaagctgaa ggccctgctc agggagctct ctgcagagga ctagcgcaaa
     1981 gcgagagaag gtcgtcgagc tgtacgtctt ccatcgcact gcgttctcct ccgatgcaga
     2041 gcgtagagaa ggactcgcaa tccggataga gttcgaccca gttcgggacc atgtccctga
     2101 ccagacgggt gatgcggctg catatcacga ggtcggggcg cagtcgcgcc acctccctgt
     2161 cgagggtctc cgggtcggcc tcgaaaacct cgacgttcgg gtgaatctcg ctgataacaa
     2221 aagcgatggt ctgacgatac gaggtgagtt cgttcgccac gagtatacgc ctggtttcca
     2281 tataagaata gtcgctttta cgagcaattt cggcatcacg aaaactagtt atctttttct
     2341 agccttattg ggctagttcg ccccggcaag ctcctcggcc tgatccacga tggagaggag
     2401 atcggggagt tggatctcgg cgtactccat gcgccttccg cctatgctgg ccacagatcg
     2461 cgcggcgttc tccgggtaga gctcgaccca tacaggcacc ctgcctttca acgcgtccgt
     2521 ggccttgctg cagatgacca tgtcgggcga gaagcggatt atggaggcat cgagggcgtt
     2581 cggctcaacg gtcgttacct cgacctcggg gcgtagctgg cctattaccg ccgctatgcc
     2641 ctcgcggtaa gcgcgcggct cgttggccat caagaccagc ggcctgccgt gccggtgccc
     2701 cgcagaggcc gcgcctgccg caggg
//
LOCUS       DATAIR010000092         6382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_282554, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000092 DATAIR010000000
VERSION     DATAIR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282554"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VHM69_02320"
     CDS             <1..340
                     /locus_tag="VHM69_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2739255.1"
                     /translation="HVVGSMHERKAMMSELSEGFIALPGGTGTMEEFFEVLTWAQLGE
                     HGKPCGLLNVAGYYDPLLDFFDQMVKERFLSKTNRSIVLVDAEPTALLGRFEEYKPPK
                     TPKWIDKSET"
     gene            complement(412..1440)
                     /gene="fba"
                     /locus_tag="VHM69_02325"
     CDS             complement(412..1440)
                     /gene="fba"
                     /locus_tag="VHM69_02325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX2739256.1"
                     /translation="MALVTMRQLLDEAANGGYGVGAFNVNNLEQVQAIMEAARETESP
                     VIIQASRGARKYANDRFLYHLMQAATEVYPEISVALHQDHGNSPETCKSAIELGFTSV
                     MMDGSLMDDGKTPADFEYNVRVTREVVEMAHEAGVTVEGELGTLGGIEDGVGSGEVNL
                     TEPDQAVEFVERTGVDALAVAIGTSHGAYKFTKEPDREVLAMHIIEEINERLPTTHLV
                     MHGSSSVPKELVDIINQYGGQIEPTWGVPVREIQEGIKHGVRKVNVDTDLRLAATGAI
                     RKAFAEDPAEFDPRYYLKPARAAMQEVVRARMEAFGQAGHAKDYDPIPLPDMAARYKE
                     QGHKLTVT"
     gene            complement(1586..2449)
                     /locus_tag="VHM69_02330"
     CDS             complement(1586..2449)
                     /locus_tag="VHM69_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2739257.1"
                     /translation="MGKRSDHRKFRRRRRWAGLILLATLALMVSGVLALTGEEPTNAQ
                     PSVSVDAVKKVKSPSVADAPEAVSEQKQDKTALLASEEEDRKTAAERNEVEREAAQKA
                     DEKRAAEKKADKKEATKEQAAHKPAPHKPAIPTPRTNDLWMSIPALGLYDNYVTNTNA
                     YSALDYGAVKLPESAFPWQDNANTYIAAHRVGWPGTGSDHQFYNLRLMEYGDKIYLGD
                     VNGTTYTYEVTEFHEITPDERWITGPQAGRDMVSLQTCIENYGDYWTMGPNWYVRYVV
                     QADRVSVDVAR"
     gene            2779..3744
                     /locus_tag="VHM69_02335"
     CDS             2779..3744
                     /locus_tag="VHM69_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739258.1"
                     /translation="MNTETERTTLGLSGESTLVASGLKKNYGDFAAVRGVDFRVSGGE
                     CFGFLGPNGAGKTTTMKMIYGAVIPTGGELTVAGLDVYHHEREIKRRIGVVPQENNLD
                     DELKVRENLLIYGRYYDLPRKVVLQRTDELLDFMQLSDKAEATVEQLSGGMKRRLLIA
                     RALINDPEIVILDEPTTGLDPQARLLVWDRLRKLAAEGKTLILTTHYMEEAARLCDRL
                     AIMERGLIIAEGTPKALVEEHVSPQVIELRTDRRSLERLLPIVEAASDAVERTGEDRL
                     LAYTEDVEALLEDVRSSGVEIENTAYRHTGLEDVFLRLTGRRLVE"
     gene            3744..4541
                     /locus_tag="VHM69_02340"
     CDS             3744..4541
                     /locus_tag="VHM69_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739259.1"
                     /translation="MNAIRIPSARGSYRVWQRNITIFRKYWKTILFPNFVEPLLYLAA
                     LGLGLGAFIQQGGINGQSYVQFVAPGLLASNAMFAASFESTFNTFVKLKIDKIYDAII
                     TTPVNAEDVVAGEYLWAGTRAALYGTAFLAMIVVLGIVFGEPLVTSWWALLLPLMLLV
                     IGIMFSVLGTLFTSLIERIDLYAYYFTLVVTPLFLLSGVFFPVDDFPAPVPQVAWFTP
                     LYHAVNVCRELATGPSPGVLVDVLWILVFTGVLALIPVQIMRRRLIG"
     gene            4569..5192
                     /locus_tag="VHM69_02345"
     CDS             4569..5192
                     /locus_tag="VHM69_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739260.1"
                     /translation="MEAEQFTVRFAGPDDDPAIASLVVEGFLDKFRPVFGKRMDRSLK
                     IMEKWIGLEHASGGVTSLVVEGYSTGELAGSVGVRTGASREDVLARGLWNSFTRNLGF
                     LRAVWATTLLSFPRYSSRSSEAYVERLVISPSFRRQGMGRRLLSAAEDLARDTGKKTV
                     GLHVTGNNLAALRLYEAEGYVEVSRQRSFLTARFMDIREWLYLRKEL"
     gene            complement(5346..>6382)
                     /locus_tag="VHM69_02350"
     CDS             complement(5346..>6382)
                     /locus_tag="VHM69_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739261.1"
                     /translation="YAFCAHFGLTRANVIGHDLGGAMAVKLAADHPDVVGRLVIVSAP
                     ADEDQIDVPTMLWLATLPVIGPIFYALGRVARPVRRAWMRPFVADADDLTDEVVDDAG
                     RSTPAAVSKTLSISRREISRGRLARQARIIKVPLLAVAGEEDQIVDPQSVGMWASGVD
                     KAEISLIDECGHLPMIERTAEFNANILAFLTGDARYLDYAHPASQKDEDAAGYEEEEA
                     ETHYLEEGEEYPTTTDSSDDTVDLTPRGMQPETRSDNVSNVVRKHEGRYPARTARHPE
                     ASADGVEERSGSRPRTRREPPDEDFLPELPDDLFDWPETRREFGTRERHRDGSPDGQG
                     DDCPEEPPRS"
BASE COUNT         1129 a         1964 c         1972 g         1317 t
ORIGIN      
        1 ccacgtcgtc ggctcgatgc acgaacgcaa ggccatgatg tccgagcttt ccgagggttt
       61 catcgccctc cccggcggca cgggcactat ggaagagttc ttcgaggtcc taacgtgggc
      121 gcaactcggc gaacacggaa agccgtgcgg cctcctcaac gtcgcgggat actacgatcc
      181 cctcctcgac ttcttcgacc agatggtcaa agagcgattc ctgagcaaga caaaccggtc
      241 gatcgtcctc gtcgatgcgg aaccgaccgc cttgctcgga aggttcgagg agtacaagcc
      301 tccaaagacc ccaaaatgga tcgataagtc ggagacgtag tacagggcaa gccgtaaagt
      361 agtaggagcg gccagccgcc agagtactct ggcggctgac acctgaagcc tctaagtaac
      421 tgtaagcttg tggccctgct ccttgtagcg ggcggccatg tccgggagcg ggatggggtc
      481 gtagtccttc gcgtggcctg cctgcccgaa ggcctccatg cgagccctga cgacctcctg
      541 catggcggcg cgggccggct tgaggtagta gcgggggtcg aactcggccg ggtcttcggc
      601 gaaggccttg cggatcgcac ccgtggcggc gagcctgagg tctgtgtcga cgttgacctt
      661 cctcacgccg tgcttgatgc cctcctggat ctcccgcacg ggcacgcccc aggtcggctc
      721 gatctggccg ccgtactggt tgattatgtc aacgagctcc ttgggcacgc tggaggagcc
      781 atgcatcacg aggtgtgtgg tgggtagccg ctcgttgatc tcctcgatga tgtgcatcgc
      841 gagcacctcg cggtccggct ccttggtgaa cttgtaagct ccgtggctcg tcccgatggc
      901 gacagcgagc gcgtcaacgc cggtcctctc cacgaactcg acggcctgat cgggctccgt
      961 caggttgacc tctcccgagc ccacgccgtc ctcgatgccg cccaaagtgc cgagctcgcc
     1021 ctcaaccgtt acaccggcct catgcgccat ctcgacgacc tcacgcgtga cccgcacgtt
     1081 gtactcgaag tcggcagggg tcttgccgtc gtccatcagc gagccgtcca tcatgacgct
     1141 cgtgaagccc agctcgatgg cgctcttgca ggtctcgggc gagttgccgt ggtcctgatg
     1201 cagggccacg gagatctccg gatacacttc cgtcgcggcc tgcatgaggt ggtagaggaa
     1261 gcggtcgttg gcgtactttc gcgccccgcg cgaggcctgg atgatgacgg gtgattccgt
     1321 ctcccgtgct gcctccatga tcgcctgtac ctgctcaagg ttgttgacgt tgaaggcccc
     1381 gacgccgtag ccgccgttcg ccgcctcgtc caaaagctgg cgcatcgtta ccaatgccat
     1441 gtgcgtaact ctcctctctc gaactgccag gatccggccc actataccgc aatgctcttg
     1501 cggaccagga cacgagcggc cagtatagcc gctctttacg catcattcca aaggtagagc
     1561 gcgaggcgct ccgggagagt atgccttatc tggctacgtc taccgagacc ctgtcggcct
     1621 gcacgacgta gcgcacgtac cagttcggcc ccatggtcca atagtcaccg tagttctcga
     1681 tgcaggtctg aagagagacc atgtccctgc cagcctgagg accggttatc caccgctcgt
     1741 ccggcgttat ctcgtggaac tccgtgactt cgtaggtata ggtagtcccg ttgacgtcgc
     1801 cgaggtagat cttgtccccg tactccatca gacgcaggtt atagaactgg tgatcgcttc
     1861 ccgtgccggg ccagcccacc ctgtgcgccg ctatgtaggt gttcgcgttg tcctgccacg
     1921 ggaaggcgga ctcgggcagc ttgacagctc cataatctag cgccgaataa gcgttggtgt
     1981 tggtcacgta gttgtcatag aggccgagcg cgggtataga catccacagg tcgttggtgc
     2041 gcggcgtagg aatcgccggc ttgtgtgggg caggcttgtg cgctgcctgc tctttggtag
     2101 cctctttctt gtcagctttc ttctcggcgg cccgtttctc gtcagctttc tgcgccgcct
     2161 ccctttctac ctcattcctc tcggccgcgg tcttcctgtc ctcctcttcg gaggcaagta
     2221 gcgcagtctt gtcctgcttc tgctcgctta cggcttcagg ggcatcggcc acactcggag
     2281 actttacttt cttcaccgcg tccacggaga cgctcggctg cgcgttcgtg ggctcctccc
     2341 cggtaagcgc gaggacaccg gaaaccataa gggccaacgt cgcgagcagg atcaaacccg
     2401 cccaccggcg gcgcctcctg aacttgcgat gatcagacct tttgcccatg caccctctcc
     2461 atccttctgc gcaaaagttg tcgttgacca gattgaacct gcgcgggatt ttaacagcac
     2521 ttttcgcaaa gtggtagagt aaataatcaa tattccttaa tacgcactaa tgtattcgca
     2581 tagacggcaa gaggtgtgtt ggcctgatta tcctgagcgc tggcgctcga attactggtt
     2641 agatcggcga gatacgatag tataattgcc gttcccgaga tcttccccgg ctgtggcgtc
     2701 cccctcctga gggtggagcg gttcgggcgc gcggtcaccg gggtcgggac gtttcgagga
     2761 tcatagagag gctgacggtt gaacacggag acagagagga ccactttggg cctatcgggg
     2821 gagtccaccc tcgttgccag tggtctcaag aagaactacg gagacttcgc tgcggtaagg
     2881 ggcgtcgact tcagggtatc aggtggagag tgtttcgggt tccttggccc caacggggct
     2941 gggaagacca cgacgatgaa gatgatctac ggggccgtaa tcccgacggg cggagagctt
     3001 acagtggcgg gcctcgacgt ctaccatcac gagcgggaga tcaaacgccg catcggggtc
     3061 gtcccgcagg agaacaacct cgacgacgag ctcaaggtca gggagaacct gctcatctac
     3121 ggcaggtact acgaccttcc acgcaaggtg gtacttcaga gaaccgacga gctcctcgat
     3181 ttcatgcagc tctcagataa ggccgaggcg acggtcgagc agctctcggg cgggatgaag
     3241 cggcgtcttc tcatcgcgcg ggccctgatc aacgaccccg agatcgtcat cctcgacgag
     3301 cctacgacgg ggctggaccc gcaggcgcga ctgctcgtct gggaccggtt gcgcaagctg
     3361 gcggccgagg gcaagaccct catcctcacg acccactaca tggaggaagc tgcgcggctg
     3421 tgcgaccggc tcgccatcat ggaaaggggt ctcatcatcg ccgaagggac gccaaaggcg
     3481 ctggttgagg agcacgtcag cccgcaggtc atcgagttga ggacggatcg caggtccctc
     3541 gaacggcttc ttcccatcgt cgaggccgcc tccgacgccg tcgaacggac cggcgaggat
     3601 cgtctcctcg cgtataccga agacgtcgag gcgctgctcg aggacgtgcg tagctcgggt
     3661 gtcgagatcg aaaacaccgc ttatcgtcac acggggctcg aggacgtctt cctgcggctc
     3721 acgggcagga ggttggtaga gtagtgaacg cgataaggat accctcggcc aggggatcgt
     3781 acagggtgtg gcagcgcaac atcaccatct tccgcaagta ctggaagacg atcctgttcc
     3841 ccaacttcgt cgagccgctg ttgtacctgg ccgccctcgg gctcgggctc ggggcgttca
     3901 tccagcaggg aggcatcaac ggccagagct acgtccagtt cgtcgcgccg ggacttcttg
     3961 caagcaacgc aatgttcgca gcctccttcg agagcacgtt caacacattc gtcaagctca
     4021 agatagataa gatctacgac gccatcataa cgacacccgt caacgccgag gatgtcgtgg
     4081 ctggggagta cctgtgggcg gggacgcggg cggcgcttta cggtacggcg ttcctagcga
     4141 tgatcgtggt tctcgggatc gtcttcggcg agccgctcgt cacgtcgtgg tgggcgttgc
     4201 tcttgccctt gatgctgctc gttatcggca tcatgttcag cgtgctgggt acgctgttta
     4261 ccagcctgat cgaacgcata gatctttacg cctactactt cacgctggtc gttacgccgc
     4321 tattcctgct ttccggcgtt ttcttccccg tggacgactt ccccgctccg gtgccgcagg
     4381 tcgcctggtt cacccctctc tatcacgctg tcaacgtgtg tagagagctc gccacgggcc
     4441 cttcgcccgg cgtcctagtg gatgtgctct ggatcctcgt cttcaccggc gtcctcgccc
     4501 tgatcccggt ccagatcatg cgccgtcggc tcatcggctg aggggcagga ctctggtagg
     4561 atggtccgat ggaggccgag caattcaccg tacgcttcgc cgggccggac gacgatccgg
     4621 cgatagcatc cctcgtggta gagggattcc tggacaagtt caggccggtc ttcggcaaga
     4681 ggatggaccg ctccctcaag atcatggaga agtggatcgg cctcgagcac gcctccggcg
     4741 gggtcacctc actggtggtc gagggatact cgacggggga gctcgccgga agcgtgggcg
     4801 tcaggacggg ggcatccagg gaggacgttc tcgccagggg attgtggaac tcgttcaccc
     4861 gcaatctcgg ctttctgcgg gccgtatggg cgacgacact gctctcgttt ccaagatatt
     4921 cctcccgctc ctcagaagcc tacgtcgagc ggcttgtgat ctccccctcc ttccggcgcc
     4981 agggtatggg acgacgcctg ctttcggcag ccgaagacct cgccagggac acaggcaaga
     5041 agaccgttgg cctgcacgtc acaggcaaca accttgccgc cctgcgcctc tacgaagccg
     5101 agggttacgt ggaagtctcg cgccagagat ccttcctaac cgcccgcttt atggacatac
     5161 gcgaatggct gtacctgagg aaggaactct gaagcgctat gcaagaagtg tacttcccac
     5221 atagcgcgct gcctgcggca gctctcagct cttcagcaat tcagtctctt ttccgggaag
     5281 ttttcgcatt tcgaatctct agctgaaggc gcgattagca gaagtattga cttctgatat
     5341 tcgcatcacg agcgaggcgg ttcttccggg cagtcatcac cctgtccgtc gggcgagccg
     5401 tccctgtgac gctcgcgtgt cccgaactcg cgtcgcgtct cgggccagtc gaagaggtca
     5461 tccggcagtt cgggcagaaa gtcctcatca ggtggctcgc gtcttgtccg cggtcgtgat
     5521 cccgagcgct cttcgacgcc gtccgcggac gcctcggggt gccgcgcagt tctggccggg
     5581 taccttccct cgtgtttgcg gacgacattg gacacgttgt cgcttcgggt ctcgggctgc
     5641 atgccgcgtg gggtgagatc caccgtatcg tccgaggagt ctgtcgtggt tgggtactcc
     5701 tcgccttctt ccaggtaatg agtctcggct tcttcctcct cgtatccggc ggcatcttcg
     5761 tccttctgcg aggcagggtg ggcgtagtca aggtagcggg catcgcccgt caggaaggca
     5821 aggatgtttg cgttgaactc agcggttcgc tcgatcatgg gcaggtggcc acattcgtcg
     5881 atgagagaga tctcggcctt gtccacgccg ctggcccaca taccgaccga ctgcgggtcg
     5941 acgatctggt cctcctcacc ggcgacggcg agcaggggga ccttgatgat ccgggcctgc
     6001 ctggcgagcc gcccgcgcga gatctcgcgc ctcgagatac taagggtctt ggagacggcg
     6061 gcgggcgtcg atctgcccgc atcatcgact acttcgtctg tgagatcgtc ggcatccgcg
     6121 acgaagggcc gcatccaggc gcgacggacc ggacgcgcta cgcgcccaag ggcgtagaag
     6181 atgggcccga tgacgggcag ggtggcgagc cacagcatgg tcggaacgtc tatctggtcc
     6241 tcatcagccg gagccgagac gataacgagg cgccctacga catcggggtg atccgccgcg
     6301 agcttgaccg ccatggcccc gccgaggtcg tggccgatga cgttggcgcg ggtcaggccg
     6361 aagtgggcgc agaaagcgta aa
//
LOCUS       DATAIR010000093         6150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_325644, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000093 DATAIR010000000
VERSION     DATAIR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325644"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHM69_02355"
     CDS             <1..315
                     /locus_tag="VHM69_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX2739262.1"
                     /translation="GADAMSATPGHSQHQLGTAVDFTNAMANYQVRRSFGRTTASTWL
                     QTHATDHGFVLAYPPGKDGTSYEFEPWHYRYIGVENAKRLEQSGLTPQEFLVREGVLP
                     NC"
     regulatory      452..526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            510..1226
                     /locus_tag="VHM69_02360"
     CDS             510..1226
                     /locus_tag="VHM69_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX2739263.1"
                     /translation="MAELPMFGITLDKVKMLSALCMGIAFAAALVVISAHSAQAQGTG
                     FRDDFDSFDSDRWLKSDHQQGLTDFDPNNVVVNSGYLRLKIPAGTTGGAEIESRDYYG
                     YGTYRARMRVADAPSAITGFFLYSGTDPFSEIDIEVYNDGTGNVDFVTYAEGQRTNAV
                     SKDFRFDPSADFHNYRIDYNPGSVQFYIDGNLRQAWTEGVPSTSMKLLVNTWFPSWLP
                     GQEPDTTRLTRVDWIDYDPS"
     gene            1356..1478
                     /locus_tag="VHM69_02365"
     CDS             1356..1478
                     /locus_tag="VHM69_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739264.1"
                     /translation="MATEYLQFIGALVFFLGILIGSYWFVYVPNRGDRTSDDDG"
     gene            1517..1981
                     /locus_tag="VHM69_02370"
     CDS             1517..1981
                     /locus_tag="VHM69_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2739265.1"
                     /translation="MQVSAKTDYALRAASELALAATEDAGPIKGDWISETQGIPKKFM
                     ENILHDLKRSGLVRTRRGASGGYWLALPAEEISLAAIIRAVEGPLANVRGEWPEAVEY
                     SGAAVHLQEVWIAVRANLRAVLESVTLADLVEGELPPSITELTRDPEAWVHH"
     gene            2058..2915
                     /locus_tag="VHM69_02375"
     CDS             2058..2915
                     /locus_tag="VHM69_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739266.1"
                     /translation="MLEFLSSATSTAPPGAYWHPGDAIWGIYQNAVFDPRHGVRLWED
                     GDDLLGFAWLEEPDGVVMQVHPRLRGSGMLEREMLDWASHQTRAVYGERAGDELWTRV
                     PEDDPRLPAFLAGLGFVRDPDHALMMFRELDGSLPRPAPPDGWTVREVGGEEEWEQRV
                     ETHREVWHPSRVTLEAYRRLRNAPGYNPSLDLVAVAPDGTFGSYCICWFDPGSRTGLF
                     EPVGTRPAHRGKGLGKSVMLEGMHRLRDLGARVAAVIAMHDNEAAAGLYESVGFRTVN
                     TERLYGKKL"
     gene            2996..4321
                     /gene="chrA"
                     /locus_tag="VHM69_02380"
     CDS             2996..4321
                     /gene="chrA"
                     /locus_tag="VHM69_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX2739267.1"
                     /translation="METETAREGTEVKPVGFWEAFWFWVKLGFINFGGPAGQIAIMHK
                     ELVEKKRWVSEGQYLRTLNFCMLLPGPEAQQVATYIGWRLHGTLGGIVAGAFFVIPSI
                     FVLWLLSYLAVAHSDVPVITGLLYGIQPVVIAIVVEAVLRIAKRALNHNLLVGFSVAA
                     FVALYFFSIPFPLVVLAAALGGLLLSRAVPEAFQVGGHGSSEEEESAVDRAEYNGRPS
                     MLRNLRLVGTFLVLWAVPVGALYLWRGSEDVLVKEALFFTGAAFVTFGGAYAVLSYIS
                     DVAVNQYGWLTADQMVQGLGLAESTPGPLIMVTEYVGFIGAYNDPGTFAPLLYGTLGA
                     LVTTYATFLPCFLFIFLLAPYIELLGNNQRIRAVLVGVTAAVVGVIANLAVFFATQVL
                     FPGGVSLEGLDLFALVVAVVSFVVLQRFKVPIYLMVPAGAVVGMVWTLL"
     gene            4306..4839
                     /locus_tag="VHM69_02385"
     CDS             4306..4839
                     /locus_tag="VHM69_02385"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEX2739268.1"
                     /translation="MDSPVGGGQKVRWLIMIYHLPREPSRHRVAVWRKLKTLGALYLQ
                     DGVAALPEDAVTREQLEWLQLRVREAGGEATLWEALPNTAAEDRDLVEEFRASREAAY
                     RELIAAAEKLRQKAMFGADDTVLLEELAGIERDFRAERRRDYFRSPLRKVADAAIREA
                     RRVLKEGAEQASREVTG"
     gene            4839..5255
                     /locus_tag="VHM69_02390"
     CDS             4839..5255
                     /locus_tag="VHM69_02390"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX2739269.1"
                     /translation="MLWATRRGCHVDRTACIWLIRRRIDPGAEFHFFRDPAEAPRGAE
                     LFDVAGARFSHRGDACSFETFLDEYELDDPVLHEISEIVHDADLMDDRYGRTESDGLD
                     AVIRGMQLALPDDVALSRHTDAIFDGLYTYLRRETL"
     gene            5257..5955
                     /locus_tag="VHM69_02395"
     CDS             5257..5955
                     /locus_tag="VHM69_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2739270.1"
                     /translation="MASLKIQVSGLGYAEILKGVDAGMPDGEITAVVGPSGAGKSTLL
                     RAINRLIEPTSGEIYLDGEPTSGLDPLELRRRVGMVFQIPALFGRSVEEAVLYGARLS
                     GRDADPGRLLEMVALDPSLARREPQALSVGQQQRVSVARALALEPEALLLDEPTSALD
                     EAARRRIEELVRDLNARLGLTMVFVSHDLSQVERVADRVVLMDDGRSVGEWSSEDFFS
                     TEGERARLFISGRL"
     gene            5957..>6150
                     /locus_tag="VHM69_02400"
     CDS             5957..>6150
                     /locus_tag="VHM69_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739271.1"
                     /translation="MEGLQTVGSALMTLGLVAVAVALSFYEKLDLEKDIGVAVVRSFV
                     QLAAIGYVIDFIFGLDNVGVV"
BASE COUNT         1098 a         1881 c         2021 g         1150 t
ORIGIN      
        1 ggagccgacg cgatgagcgc gacgccggga cacagccagc atcaactcgg taccgccgtt
       61 gacttcacga acgcgatggc aaactaccag gtacgccgga gcttcgggcg cacaaccgct
      121 tcgacgtggc tgcaaaccca cgcaaccgat cacggcttcg tcctcgccta cccgccgggc
      181 aaggacggaa cgagctacga attcgagccc tggcattacc gctatattgg cgtggagaac
      241 gcaaagcgtc tcgaacagag cggcctgacg ccgcaggagt tccttgtccg cgagggagtc
      301 ttgccaaact gctgaggctt ctgacacagc ggccctcctc gagttcaccg acggtataga
      361 aggcacaagc tcaggatgtt cgcgagccga cagggcatcg cgggaccgcg acgttgggag
      421 cccgactcct caccacctga tacactcaaa cgttacgaaa aggcaaaccc gccgcgaggc
      481 ggggacgcaa agctacggac ctccaggagg tggccgagtt gccgatgttc ggcatcacgc
      541 tcgataaggt caagatgctt tctgcgctct gtatggggat cgctttcgca gcggcgctcg
      601 tggtcattag cgcgcacagc gcccaggcgc aaggaaccgg cttccgcgac gacttcgact
      661 ccttcgactc ggaccggtgg ctcaagagcg accaccagca ggggctcacc gacttcgacc
      721 cgaataacgt cgtggtcaac agcggatatt tgagactcaa gatcccggcc ggaacgaccg
      781 gcggtgcgga gattgagtct agggactact acggctacgg tacttaccgc gcccgcatga
      841 gggtggctga cgccccctct gccatcaccg gcttcttcct ctacagcggc accgacccct
      901 tctctgagat agacatagaa gtctacaatg acggcacggg caacgtggat ttcgtcacgt
      961 atgcagaagg gcagaggaca aacgccgtga gcaaggactt ccgcttcgat ccgagcgcgg
     1021 acttccacaa ctaccgcatc gactacaatc ccggctccgt gcagttctac atcgacggga
     1081 acctcaggca ggcgtggacg gaaggcgtcc ctagcacatc gatgaagctt cttgtgaaca
     1141 cgtggttccc tagctggctt cctgggcagg agccggacac gaccaggctg acgcgcgtcg
     1201 actggatcga ctacgatcca agctgatccc tcttctcccc tgaccgtcgc gcaagagttg
     1261 agcaatcctc aagtagaccg gcggcgaagc gggcgcggct gtcgtagaat tgtgcaacga
     1321 ggcgtcggac ggtttgggaa cctgaaggag gttggatggc gacggagtac ctgcagttca
     1381 tcggggcgct ggtgttcttc ctggggatac tgatcgggtc ttactggttc gtctacgtgc
     1441 cgaaccgcgg cgacagaacc agcgacgatg acggttgagg gcgaggatag acgccttcag
     1501 gggagcgtga ggtagcttgc aggtttctgc caagaccgac tacgcgctca gggcagcctc
     1561 cgagctggcg ctggcggcga ccgaggacgc cggcccgata aagggggatt ggatctccga
     1621 gacccagggc atcccgaaga agttcatgga gaacatcctg cacgacctga aacgctccgg
     1681 gctcgtccgt accagacggg gcgcctcggg cggctactgg ctcgcgcttc cagcggagga
     1741 gatctcactc gccgccatca tccgcgccgt ggagggacca ctggcgaacg tcaggggcga
     1801 gtggcccgag gcggtcgaat actccggcgc cgccgtgcac ctgcaagagg tctggatcgc
     1861 cgtccgcgca aacctgcgcg ccgtcctcga atccgtcacc ctcgccgacc tcgtcgaagg
     1921 ggaactccca ccctcgatca ccgagctcac ccgcgacccc gaagcctggg tacatcacta
     1981 gcttgccggc tcccagactt tagcttcctg gcggcttgac gtcccgtccc tacggtggag
     2041 agggtgatct cgcccgaatg ctggagtttc tgtcttcggc cacctccacc gctccgcctg
     2101 gggcttactg gcaccctggc gacgcaatct gggggatcta ccagaatgct gtcttcgatc
     2161 cacgccacgg ggtacggctc tgggaagacg gcgacgacct actgggcttc gcgtggctcg
     2221 aagaaccaga cggtgtggtg atgcaggtgc atccacgcct tcgcggctct ggcatgctcg
     2281 aaagggagat gctcgactgg gcctcacacc agactcgcgc cgtgtatgga gagcgggctg
     2341 gcgacgagct gtggacacgc gtccccgaag acgatccacg gctcccagca ttcctcgccg
     2401 gtctcggctt cgttagagat cccgaccacg cgctgatgat gttccgtgag ctggacggct
     2461 cgctaccccg gcccgcacca cctgatgggt ggaccgtacg cgaggtaggc ggggaagagg
     2521 agtgggagca gcgcgtcgag acccaccgcg aggtgtggca tccttcccgc gtcaccctcg
     2581 aagcgtaccg caggctcagg aatgcgccgg gctacaatcc gagcctcgat ctcgtcgctg
     2641 tcgctccaga cggtacgttc ggttcctact gtatctgctg gtttgatcca gggagccgta
     2701 caggcctgtt cgagcccgtc ggtacccgcc ccgcgcatag gggaaagggt ctaggcaagt
     2761 ccgtcatgct cgaaggtatg cacaggctgc gggatctggg cgcccgtgtc gccgccgtta
     2821 tcgcgatgca cgacaatgaa gctgctgccg gactgtacga gtccgtcggt ttcagaacgg
     2881 tcaacacgga acgcctctac ggcaagaagc tgtagaacgg acgttttcct ccttgacatc
     2941 cgatgtaacc gtggttactc tggtctggat tgttcactag acgagtggag atagattgga
     3001 aaccgagacg gcaagagagg ggacggaggt caaaccggtc gggttctggg aggccttctg
     3061 gttctgggtg aagcttgggt tcatcaactt cggggggcct gcgggccaga tcgcgatcat
     3121 gcacaaggag ctggtggaga agaagcggtg ggtctccgag ggccagtact tgaggacgct
     3181 gaacttctgc atgttattac cggggccgga agcccagcag gtcgcgacct acatcgggtg
     3241 gaggctgcac gggacgttgg gggggatcgt ggccggtgcc ttcttcgtga taccgtcgat
     3301 cttcgtgttg tggctcctct cgtatctggc cgtagcccac tccgacgtac cagtgattac
     3361 gggtcttttg tacgggatcc agccggtcgt tatagccatc gtcgtcgagg cggtactgag
     3421 gatcgcaaag cgggccctga accacaacct gctcgttggt ttctcggtcg cggcgttcgt
     3481 cgccctctac ttcttttcga tccccttccc gctcgtggtg ctcgccgccg ccctcggcgg
     3541 gctgctcctg agccgcgcgg tgcctgaggc cttccaggtc ggtggccacg gctcctcgga
     3601 ggaagaagag tccgcggtag accgggccga atacaacggc cggccatcga tgctgcgcaa
     3661 cctgaggctc gtgggcacct tcctcgtgct ctgggcggtc cctgtgggcg ccctctacct
     3721 gtggcgcggg agcgaggacg tgctcgtgaa agaggcgctg ttcttcacgg gggcggcctt
     3781 cgtcacgttc gggggggcgt atgccgttct cagctacatc tccgacgtgg ctgtgaacca
     3841 gtacgggtgg ttgacggcag accagatggt gcagggactc gggctcgccg agtcaacgcc
     3901 ggggcccctg atcatggtca ccgagtacgt cggctttatc ggggcgtaca acgaccctgg
     3961 caccttcgcc cccctgctct acgggacgct cggggcgttg gtaacgacct acgcgacctt
     4021 cctgccgtgc ttcctgttca tcttcctgct cgccccttac atagagctgc tgggcaacaa
     4081 ccagaggatc agggccgtcc tcgtgggggt cacggctgcg gtcgtaggcg tgatcgcaaa
     4141 cctcgcggtg ttcttcgcca cgcaggtcct gttccccggc ggcgtctctt tggaggggct
     4201 ggatctcttc gcactcgtgg tcgccgtggt ctccttcgtt gtattgcaga ggttcaaggt
     4261 cccgatctac ctcatggtcc ctgccggcgc cgtcgtgggg atggtatgga ctctcctgta
     4321 gggggcggac agaaggtacg atggctcatt atgatctacc atctcccccg tgagccttcc
     4381 cggcacaggg tcgccgtgtg gcgcaaactg aagacgctgg gagccctcta cttacaggat
     4441 ggagtcgccg cgttgcccga ggacgccgtt acccgcgagc aactggagtg gttgcagctc
     4501 cgcgtcaggg aggccggggg agaggcgacg ctttgggagg ccctgcccaa taccgccgcc
     4561 gaggacagag atctcgtcga ggagtttcgc gcctcccgcg aggcggcgta cagggagctc
     4621 atcgccgccg ccgagaagct aagacagaag gctatgtttg gcgcggacga tacggttctt
     4681 ctcgaagagc ttgccgggat cgaacgcgac ttcagggcgg agcgccgccg cgactacttc
     4741 cggtcaccgc tgcgtaaggt ggcagacgct gcgatccggg aggcgcgacg cgttttgaag
     4801 gaaggcgcgg agcaggcgtc gcgggaggtg acaggataat gctctgggca accaggcggg
     4861 gatgccacgt cgacaggact gcctgcatat ggttgatccg tcgccgcata gaccccggcg
     4921 ccgagtttca tttcttcagg gacccggcgg aggctccacg cggggccgag ctcttcgacg
     4981 tggcgggggc caggttctcg caccgtgggg acgcctgttc cttcgagacc ttcctcgatg
     5041 agtacgaact cgacgacccg gtgctgcatg agatctccga gatcgtccac gatgccgacc
     5101 tcatggatga caggtacggc cgcacggagt ccgacgggct ggacgccgtg atccggggta
     5161 tgcaactcgc cctccccgac gatgtggccc tctcccgcca cacggacgcc atcttcgacg
     5221 gtctctatac gtatttgcga agggagacgc tctagcatgg cctccctcaa gatacaagtc
     5281 tccgggctcg gttacgcgga gatcctcaaa ggcgtcgacg cagggatgcc ggacggggag
     5341 atcaccgctg tcgtggggcc ttcaggggcg gggaagagta ccctgctacg cgccatcaac
     5401 cgcctcatag agccgacatc aggcgagatc tatcttgacg gggagccgac gagcgggctt
     5461 gacccgctgg agctgcggcg gcgggtcggg atggtcttcc agatccccgc cctcttcgga
     5521 cgttcggtcg aggaggctgt gctctacggt gcgcgcctct ccggcaggga cgctgacccg
     5581 ggacggcttc tggaaatggt ggcgttggat ccatcgctgg cgcggcgcga accgcaggcc
     5641 ctctctgtcg gacagcagca gcgagtctcc gtggcccgcg ccctcgccct ggagccggag
     5701 gcgctcttgc tcgacgagcc aacgagcgcg ctcgatgagg ccgcgcggcg caggatcgag
     5761 gagctcgtcc gggatctgaa cgccaggctg ggactgacga tggtcttcgt cagccacgac
     5821 ctttcccagg tcgagcgcgt cgccgacagg gtcgtgttga tggatgacgg gagaagcgtt
     5881 ggggagtgga gcagtgagga cttcttctcc acagagggcg agcgtgcgcg actgttcatc
     5941 tcggggagac tgtaggatgg aagggttgca gacggtgggc tcggcgctca tgaccctcgg
     6001 gctcgtagcc gtggcggtgg ctctcagttt ctacgagaag cttgacctgg agaaggacat
     6061 cggtgtcgcc gtggtccgct ccttcgtgca actcgccgcg atagggtacg tcatagactt
     6121 catcttcgga ctcgacaacg tcggggtggt
//
LOCUS       DATAIR010000094         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_233448, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000094 DATAIR010000000
VERSION     DATAIR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233448"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VHM69_02405"
     CDS             <1..661
                     /locus_tag="VHM69_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2739272.1"
                     /translation="FEEAGLRTVERRFGPKDTIFTPGDPDDQLYFLLSGTVRLYKIYG
                     DYKEATTALLRDGGVFGKLNLVEGRWQDVFAEAVTEARVASIQKASIERVIKSDPGFA
                     LKLFSTFSERLRQSDEVIESLLHREVSTRLATLLMNLGERFGENNGAGIQIDVRLTHQ
                     DLANMIASTREAVSKVMSELQREGVIESRNRRISILNEGALAEHASRLSGLSIDGQAK
                     I"
     gene            802..1395
                     /locus_tag="VHM69_02410"
     CDS             802..1395
                     /locus_tag="VHM69_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2739273.1"
                     /translation="MTLGGWRERLGSLSVRRAELDDNELAARTLAGDLRSYEELVSRY
                     ERLVGRVVYPYARREISVEDLVQETFLRAYDRLETFNPDYRFKTWLLAIANNLGVDTL
                     RRRREIVEFNPEAHAPVSRSPEAEAVDAERSRSVREAIATLPETYGVPLILRYTEGLS
                     YAEIAEVLSITVPAVKSRLFRARNMLAGRLEEAGERE"
     gene            1392..1889
                     /locus_tag="VHM69_02415"
     CDS             1392..1889
                     /locus_tag="VHM69_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2739274.1"
                     /translation="MKGLPCDPEPIFELADGSLAPKEEREVRAHLRGCRDCLALYEKE
                     LRLNASLGSLEFEETRSVCPGVAMALPTRSIKARLLWAGLAVVLLLTTSMTLLLYGTN
                     LAGLVVDALNFFLGFVAGFADVVQIVIAAIGPVLLVAFAVGALVDLVIAAVYLSVSRR
                     RAREA"
     gene            1886..2929
                     /locus_tag="VHM69_02420"
     CDS             1886..2929
                     /locus_tag="VHM69_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX2739275.1"
                     /translation="MSLSVPYLRVNALPLVTAVILGLVVLLPIPAHAAEKRLVGDVVV
                     GSGDVETGVSTLAGDVEIRGRVENDVRSVSGKVYVYANGVVEGDIDAGNGDVEIEGPV
                     DGDVRAEFGDVYVNAPVRGDVDVGRGDVTFGPRAVVDGDVELGSGKFTGDEGAVEGAI
                     ISRGMASDFHESEGPDILGFMGWLFAALAFAACTVLAAVLAPGPLAAAARRAEESPGR
                     SFVYGLVSLPAFFVLCVVLAVSIVGIPLLVLLAPAYLALLFFGALVAAFFIGTRLLMV
                     TGRYRVGNALAAVVGALILAATTLIPLLGDLILFALVLLGTGAAIQALFSRRRPRAAY
                     PSYEDYVRDRVGG"
     gene            complement(2956..>3357)
                     /locus_tag="VHM69_02425"
     CDS             complement(2956..>3357)
                     /locus_tag="VHM69_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739276.1"
                     /translation="RVAFLERSELRLHLYLKGTETLVGSSGLHRIDWEVPKFEIGYWC
                     RTLFTGRGYTTEAVRGITAFAFDALGARRVEIRCDSRNLPSARVAERAGFKLEGELRN
                     NEVATDTGLRDTLIRAMIPEERGTSRLYDIP"
BASE COUNT          553 a         1047 c         1100 g          657 t
ORIGIN      
        1 cttcgaggag gctggtctca ggaccgtgga gaggcgcttc ggtccgaagg acacgatctt
       61 tacgccggga gatcccgacg atcaactcta cttcctgctc tctggcacgg tgcgtctcta
      121 caagatctac ggcgactaca aggaggccac gacagcgctc cttagagacg gtggcgtctt
      181 cggcaagctc aacctcgtcg aagggcgctg gcaggacgtt tttgctgaag ccgtgacgga
      241 ggcgagggtc gcgagcatcc agaaggcatc catagagcgg gtcataaaga gcgacccagg
      301 cttcgccctc aagctcttct ccactttctc cgagcggctc aggcagtccg acgaggtgat
      361 agagagcctg ttgcacaggg aggtctcgac ccgtctcgcg acgcttctca tgaacctcgg
      421 cgagcgcttc ggggagaaca acggggccgg tattcagata gacgtgcgtc tcacccacca
      481 ggaccttgcc aacatgatcg cctccaccag ggaggccgtc tccaaggtga tgagcgagct
      541 ccagcgcgaa ggcgtgatcg agagtcgcaa ccgcaggatc tcgattttga acgagggcgc
      601 cctggccgag cacgcatcca ggttgtccgg actgagcatc gacggccagg ccaagatcta
      661 gccgtcctct ggccgcgctt ttagacggct ccctcttcgg ccatgacccc ctgagctcgc
      721 atggaccgaa ttgcgtgtct ctggggaaac tttctgatgc cgggggcgta tactcatcgt
      781 agtatggagt acacatccac agtgaccttg ggtggttggc gggagaggct cgggagtctg
      841 tcggtccggc gtgcggagct cgacgacaac gagctcgccg cccgcacgct cgcgggggac
      901 ctgcgttcct acgaggagct ggtaagccgc tacgagcggc tggtcgggcg cgtcgtctac
      961 ccttatgccc ggcgggagat ctccgtcgaa gacctggtgc aggagacgtt cctgcgcgct
     1021 tacgaccgtc ttgagacctt caaccccgac taccgcttca agacgtggct gctggcgatc
     1081 gccaacaacc tcggcgtgga caccctgagg cgcagaaggg agatcgtcga gttcaacccg
     1141 gaggctcacg ctcccgtctc gcggagcccg gaagccgagg ccgtggacgc cgaacgttcg
     1201 aggagcgtga gggaggcgat cgcaaccctg cccgagacct acggcgtccc tctcatcctc
     1261 cgctacaccg agggcctcag ctacgccgag atagccgagg tgctctcgat cacggtccct
     1321 gcggtaaaga gccgcctgtt ccgcgcccgc aacatgctcg ccggaagact ggaagaggcg
     1381 ggtgagcgag aatgaaaggt ctcccatgcg accccgagcc catcttcgag ctcgccgacg
     1441 gctcgctggc gccgaaggaa gagcgggagg tcagggccca cttgagaggt tgcagagatt
     1501 gcctggcact ctacgagaag gagttgcggc tcaacgcatc cctcggctcc ctggagttcg
     1561 aggagaccag gtctgtgtgc cctggcgtcg cgatggcgct gccgacacgg tccatcaagg
     1621 ccaggctcct ttgggccggg ctcgccgtgg tcctgctgct gacgacctcg atgaccttgc
     1681 tcctctacgg gacgaacctc gcgggcctgg tcgtcgacgc gttgaacttc ttcctgggat
     1741 tcgtggccgg cttcgcggac gtggtccaga tagtcatcgc cgctatcggt ccggtgctcc
     1801 tggtcgcctt cgccgtcggc gctctcgtgg acctcgtcat agccgcggta tacctctcgg
     1861 tctcccgccg gcgcgcccga gaggcatgag cctctccgta ccgtacttgc gggtcaacgc
     1921 cctgccgttg gtcacagcag tgatcctcgg tctcgtcgtt ctccttccga ttcccgctca
     1981 cgcagccgag aaacggcttg taggcgacgt cgtggtgggc tccggggacg ttgagaccgg
     2041 agtttcgacc ctagctggtg acgtcgagat acgcggaaga gtagaaaacg acgtgcgttc
     2101 cgtatcgggc aaagtgtacg tatacgcgaa cggtgtggtg gagggcgaca tagacgccgg
     2161 aaacggggac gtggagatcg aaggtcccgt cgacggcgat gttcgggccg agttcggaga
     2221 cgtatacgtg aacgccccgg tcagggggga tgttgacgtg gggcggggcg acgtaacctt
     2281 tggcccgagg gcagtggtgg atggggacgt cgagctcggg agtggcaagt ttacggggga
     2341 cgagggcgcc gtggagggcg ccataatatc cagggggatg gcctctgact tccacgagtc
     2401 cgaaggacca gacatactcg gcttcatggg ctggctcttc gcggcgctcg cgttcgcggc
     2461 gtgtaccgtg ctggccgcag tcctggcgcc gggaccgctg gccgcggcgg cgcgcagggc
     2521 ggaggagtct ccggggagat ccttcgtcta cgggctggtg tcgctgccgg cgttcttcgt
     2581 gctctgcgtc gtgctcgcgg tcagcatagt cgggataccg ctccttgtgc ttctcgcgcc
     2641 ggcttatctg gcgctacttt tctttggggc gctggtcgcc gctttcttca ttgggaccag
     2701 gctcttgatg gttacgggac gctacagggt tggcaacgcc ctggccgccg ttgtgggggc
     2761 cctgatcctc gctgcgacga ctttgattcc cttgctcggc gacctgatcc tgtttgccct
     2821 cgtcctgctc ggcacgggag ccgctatcca ggctcttttc tctcgtcgcc gtccacgcgc
     2881 cgcctaccca tcctacgaag actacgttcg cgaccgcgtc ggcggctgac cgcacgttgt
     2941 cctcggctct ggcggtcagg gaatgtcgta caagcgtgag gtcccgcgtt cttctgggat
     3001 cattgcgcgg atcagagtgt ctctaagccc tgtgtcggtt gcgacctcgt tgttgcggag
     3061 ctcgccttcg agcttgaagc cggcacgctc ggcgactctc gcgctcggga ggttgcgtga
     3121 gtcgcaccgg atctcgaccc gcctggcacc gagggcgtcg aaggcgaagg ccgtgatgcc
     3181 ccgcaccgct tcggtcgtgt agccgcggcc cgtaaacaga gtccggcacc agtagccgat
     3241 ctcgaacttc ggcacttccc aatctattct atgcaacccg ctgctcccta ccagcgtctc
     3301 cgtccctttg aggtaaaggt gcagccggag ctccgaccgc tccaggaaag ctaccct
//
LOCUS       DATAIR010000095         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_375043, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000095 DATAIR010000000
VERSION     DATAIR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375043"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1013
                     /locus_tag="VHM69_02430"
     CDS             135..1013
                     /locus_tag="VHM69_02430"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2739277.1"
                     /translation="MDKATDAVIIGNPNSGRAGDEDYLERFAKILRSAGLRIEVLNTE
                     YPDHATELAALAGDRLVVAAGGDGTVNEVLNGLSPGATLGVLPLGTANVLARELGLPL
                     DAEAACQRILKGEISRVDFGVATNEEGAERRFACMAGIGFDAKVVGTVTPRQKRYLRG
                     LAFQVTAFRVLFGNKFPPLEISHGDKVHVARFAIVANGHYYGGDFKISGPGLLTSGEL
                     ETVLVERVSVLLRPDVFAGIMTKRPLNRAMKSFTSTEVHATAKGAEVPVQLDGELWGG
                     LPMSFRIEPAALNVIR"
     gene            complement(1033..2064)
                     /locus_tag="VHM69_02435"
     CDS             complement(1033..2064)
                     /locus_tag="VHM69_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX2739278.1"
                     /translation="MTFVRAMILDAPGEPLRAAEAPEPEPGAGQVLLRVHCCAVCRTD
                     LHVVDGELPDPRLPLIPGHQIVGTVEKLGEHVTSFALGDRVGVPWLGWTDGECRYCLS
                     GRENLCENARFTGYQIDGGYAEYAVADARFCFPIHESYHDLQAAPLLCAGLIGHRSLR
                     FAGDAERLVLYGFGASAHIVAQVAAHQGRRVFAFTSPGDEEAQNFARELGAEWAGSSL
                     EAPPEELDAAIIFAPVGSLVPAALGVVARGGTVVCAGIHMSDIPSFSYDLLWGERSVR
                     SVANLTRRDGHEFLALAPEVPVRTEVVPFALDDANEALDALRGGRVRGAAVLVVNSPG
                     NGREEIPVG"
     gene            complement(2245..>2536)
                     /locus_tag="VHM69_02440"
     CDS             complement(2245..>2536)
                     /locus_tag="VHM69_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2739279.1"
                     /translation="YCPVVAVLGEGLIREKTLSNVEETIARGANVIAVAGEGDRAAHR
                     MARIVLAVPEAPEILAPFVCSVPLQLLAYHVAKDRGLDVDKPRNLAKSVTVE"
BASE COUNT          484 a          833 c          813 g          406 t
ORIGIN      
        1 tcggggtgcg atcgagggcg aactgcatcc tctcgacgtc aacgacatcg accccgatcc
       61 ctggtacgac gaatccatcc acggtgcgta tgttacggca cattggcctc aggcaaaagg
      121 gatatagtat gcggatggat aaggcaaccg acgcggtcat catcggcaac ccgaattcgg
      181 gacgcgcggg cgatgaagat tatctcgagc gattcgcgaa gatcctgcgc tccgcgggcc
      241 tgcgaataga ggtactgaac accgaatatc ccgaccacgc caccgagctc gccgccctcg
      301 cgggggaccg cctcgtcgtg gccgccggcg gcgatggcac ggtgaacgag gtattgaacg
      361 gactctcgcc gggagcaact cttggagtcc tgccactcgg cacggcgaac gttctggcga
      421 gggagctcgg cctgccgctc gacgccgagg cggcctgcca gcgcatcctg aaaggggaaa
      481 tctcccgcgt ggacttcggc gtcgcgacga acgaagaggg cgccgagcgc cgcttcgcgt
      541 gcatggccgg cattggcttc gatgcgaagg tcgtgggcac cgtgacgccg cgccagaagc
      601 gctacctgcg ggggctggcc ttccaggtta cggcgttcag ggtgcttttc gggaacaagt
      661 tccctccgct ggagataagc cacggcgaca aggtccacgt cgcgaggttc gccatcgtcg
      721 ccaacggcca ctactacggc ggggacttca agatctcagg ccccggactc ctgacgagcg
      781 gcgagctcga gaccgtcctg gtcgaacgcg tgagcgtcct attgagaccc gacgtgttcg
      841 ccgggatcat gacgaaaaga ccactcaacc gcgcgatgaa gtcctttacc tcgacggagg
      901 tacacgccac ggccaagggg gcagaggtgc ccgtacaact cgacggagag ctctggggcg
      961 gattgccgat gtccttccgc atcgagccag ccgccctgaa cgtcatccgc tagcgacgct
     1021 cgggcaaggc actcaaccca cggggatctc ttcgcggccg ttaccgggcg agttcacgac
     1081 gagcacggca gcaccccgca cccgtccgcc gcgcagagca tcgagagcct cgttggcatc
     1141 gtcgagggcg aacggaacga cctcggtgcg gacaggcacc tcgggggcga gcgcgaggaa
     1201 ctcgtggccg tcccggcgcg tcaggttggc caccgagcgc accgagcgct ctccccacag
     1261 cagatcgtag gagaaggagg ggatgtcgct catgtgtatg cccgcgcaga ccaccgtgcc
     1321 tcccctggcg acgaccccga gcgccgcggg tacgagggag cccacgggtg cgaagatgat
     1381 cgccgcgtcc agctcctccg gcggcgcttc tagagaactc cctgcccact cggcgccaag
     1441 ttccctggcg aagttctgcg cctcctcgtc tccagggctt gtgaaggcga agacccgcct
     1501 gccctgatgc gcggcgacct gggctacgat gtgggccgat gccccgaagc catagaggac
     1561 gagcctctcg gcgtcgccgg cgaagcgcaa tgaacggtga ccgatcaagc ccgcgcaaag
     1621 aagcggcgcg gcctgaagat catggtagct ttcgtggata gggaagcaga atcgcgcatc
     1681 ggccacggca tactctgcgt aaccgccgtc gatctggtac ccggtgaacc tcgcgttctc
     1741 acacaggttc tctcgcccgg agaggcagta acggcactcg ccgtcggtcc atcccagcca
     1801 cggcaccccg accctgtcgc ccaaagcgaa gctggtgacg tgctcgccga gtttctccac
     1861 ggtaccgacg atctggtggc cggggatcag gggcagcctg gggtcgggca actccccgtc
     1921 cacgacgtga agatccgtcc tgcacacggc gcaacagtgg acccgcagaa ggacctgtcc
     1981 agcgccgggc tccggctccg gcgcctcggc ggcacgcagc ggctcgcccg gagcgtcaag
     2041 tatcatcgcg cgcacgaacg tcacctcctg ctcgcattca gttcgtagtc agaactccat
     2101 tgtagtagta aggaacaggc tacagcgctt ctcggaaata atgatcccac aacaggtcga
     2161 gagaacgccc ttctggctca aatcgctcca agacaaaatg ctgaaaagcc gcctggggta
     2221 acccccaggc ggcgtgctga gaagctactc gaccgttacg ctcttggcga ggttgcgcgg
     2281 cttgtccacg tccaggccgc ggtccttcgc cacgtggtag gcgaggagtt gcagcggtac
     2341 gctacagacg aaaggcgcaa ggatctccgg tgcctctgga acagcgagca cgatgcgggc
     2401 catcctgtgc gctgccctgt ctccctcgcc ggcgacggcg atcacgttcg cgcctcttgc
     2461 gatggtctcc tcgacgtttg agagggtctt ctcgcggatg agaccctcac cgagcacggc
     2521 aacgacgggg cagtat
//
LOCUS       DATAIR010000096         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_375069, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000096 DATAIR010000000
VERSION     DATAIR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375069"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHM69_02445"
     CDS             <1..264
                     /locus_tag="VHM69_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HEX2739280.1"
                     /translation="LDDSTSAVDAETEAAIQETLDRLMREKHRTVFVIAQRVSTVRDA
                     DLILVLEGGSIAARGTHQELLRESELYNEILGSQLLSPSETAA"
     gene            323..943
                     /locus_tag="VHM69_02450"
     CDS             323..943
                     /locus_tag="VHM69_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX2739281.1"
                     /translation="MKIAVIGAGRIGANAARLFARAGHEVVISNSRSPETLSGIVEEI
                     GNDTQAATVEEAADFGDVVLEAIPFGRFEELPANRLEGKIVIDASNYYPHRDGEIDLD
                     GLTSSEAVARHLSGARVVKAFNTMHYERLANEGRPGFPVEQRQVIFVAGDDEEAKGIV
                     SRLIEEIGFSPIDTGSLKESHRQEPGAPIHNNPMTPERARAMLADD"
     gene            956..1498
                     /locus_tag="VHM69_02455"
     CDS             956..1498
                     /locus_tag="VHM69_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2739282.1"
                     /translation="MNETTEDRVPRDYIAFLRGLRAVRSFRPDPVPREVVDDILEVAR
                     WSGSAGNVQPWEILVVRDRDTLRALASFEGYARHLAGAPLGIVLVMAGERRERETYDE
                     GRLAERIMLAASAHGVGSSIGWIVGSGRDAARDLLGIPEALMVRTAISLGYPEARRPR
                     SGRGQARKALGDIVHEERYG"
     gene            1506..1886
                     /locus_tag="VHM69_02460"
     CDS             1506..1886
                     /locus_tag="VHM69_02460"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEX2739283.1"
                     /translation="MNDSFDTETLQLLDRTREVRIETRRDADSPVHRTIIWVVVVEGA
                     VYVRSVRGQKGRWYREISSNPEGALHAGGRRIPVRAALAADTNTVDAVSEAFRAKYER
                     SSPASTAAMLRPDVLPTTLKLSPA"
     gene            1901..2275
                     /locus_tag="VHM69_02465"
     CDS             1901..2275
                     /locus_tag="VHM69_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2739284.1"
                     /translation="MIHEHYARSHHFDLRLEREGVLVSWAVPKGMPTDTKKNRLAMHV
                     DDHPLSYLDFEDATPVEGGPEGAVKVSIWDRGTYEAEQFDEDMVIVVFRGGRLEGRYA
                     IFRTGGKNWLVHMMKSSEGDED"
     gene            2355..>2606
                     /locus_tag="VHM69_02470"
     CDS             2355..>2606
                     /locus_tag="VHM69_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2739285.1"
                     /translation="MRPYAGKFALVFGLVVLGALTQAGGPWLIGRAIDRYILRGDVEG
                     LSRTMLLLLGVYVLGAVASRGQVYQVGSIGQRLLASMRAR"
BASE COUNT          498 a          831 c          870 g          407 t
ORIGIN      
        1 ctggatgaca gcacatcggc tgtcgacgcc gagacggagg cggcgattca ggagacatta
       61 gaccgcctca tgcgcgagaa acaccgcacg gtcttcgtca tcgcccagag ggtctcgacc
      121 gtacgggacg cggacctgat cctcgtcctc gaagggggca gcatcgccgc gcgcggcacc
      181 caccaggaac tactgcgcga gagcgaactg tacaacgaga tcctcggctc ccaactgctc
      241 tccccctcgg agacggccgc atgaagccag ggctcggaac ggtggaccgg gatggtgcgt
      301 acgatcgaag gaaggactga gcgtgaagat agcggtgatc ggcgccggcc gcatcggcgc
      361 gaacgcggcg aggctcttcg cgcgggcagg ccacgaggtg gtgatcagca actcccgcag
      421 ccccgagaca ctgtcgggga tcgttgagga gatcggcaac gacacgcagg ccgcgaccgt
      481 agaagaagcg gcggacttcg gcgacgtagt gctggaagcc atccctttcg ggcgcttcga
      541 ggagttgccg gccaaccgtc ttgaaggcaa gatcgtgata gacgcctcca actactaccc
      601 gcatagggac ggtgagatcg atctcgacgg actcacgtcc tcagaggccg tagcccgtca
      661 cttgagcggc gcgcgggtgg tcaaggcgtt caacacgatg cactacgagc ggcttgcgaa
      721 cgaggggagg ccggggttcc ccgtcgagca gcgtcaggtc atcttcgtcg ccggcgacga
      781 cgaggaggcg aagggcatcg tctcgcgcct gatcgaggag atcggcttct ctcccatcga
      841 cacaggatcg ctgaaggaaa gtcacagaca ggaaccaggc gccccaatcc acaacaaccc
      901 catgacgcct gagcgggcgc gcgcgatgct cgccgacgac tgacaggaga gaaagatgaa
      961 cgaaacgaca gaagatcgcg tccccagaga ctacatcgcc ttcctccgcg gtctgcgcgc
     1021 cgtccggtcg ttccgtcccg accctgtgcc gcgggaagtg gtagacgaca tcttggaggt
     1081 ggcccgctgg tcggggagcg ccggtaacgt gcagccgtgg gagatcctgg tcgtccgcga
     1141 cagggacacg ctacgtgctc tcgcctcgtt cgagggctac gcccgccacc tggcgggagc
     1201 gcctctcggg atagtgctcg ttatggcggg cgagcgccgc gagcgggaga cgtacgacga
     1261 ggggcggctg gccgagcgga tcatgctcgc cgccagcgcg cacggcgtcg gatccagcat
     1321 cggttggatc gtcggctctg gcagggacgc cgccagagac ctgctcggca taccggaagc
     1381 actgatggtg cggaccgcga tctccctcgg ctaccccgag gctcgccggc cccgctccgg
     1441 acgcggacag gcgcgcaagg ccctcggcga catcgtccac gaagaaaggt acggttagga
     1501 gccacatgaa cgattccttc gacacagaaa ccctgcagct cctcgacaga acccgtgagg
     1561 tgcgcatcga gacccgccgt gacgcggact caccagtaca ccgcaccatc atctgggtcg
     1621 tcgtcgtgga aggcgcagtc tacgtgcgct cggtacgcgg ccagaagggc cgctggtacc
     1681 gtgagatctc ctccaacccc gagggcgctc tgcatgccgg cgggcgccgc atccctgtgc
     1741 gcgccgccct cgctgccgac acgaataccg tcgatgcggt cagcgaagcc ttccgggcta
     1801 agtacgagag atcctcaccc gcatcgaccg ctgcaatgtt gcgcccggac gttctgccaa
     1861 cgaccttgaa actctcgccc gcctaggtat gcctcggttc gtgatccatg agcattacgc
     1921 aaggagccac cacttcgatc tacggctcga aagggaaggc gtcctcgtct cgtgggccgt
     1981 gccgaagggt atgccgaccg acacgaagaa gaaccgcctc gccatgcatg tcgatgacca
     2041 cccgctatcg tacctggact tcgaggacgc gacgccggtc gagggaggtc ccgaaggcgc
     2101 ggtcaaagta tccatctggg accgcggcac ctacgaagcc gagcagttcg acgaggacat
     2161 ggtgatcgtc gttttccgag gcgggcgtct cgaaggcagg tacgcgatct tccgcaccgg
     2221 aggaaagaac tggctggtcc acatgatgaa atcttccgag ggggacgagg attaggggga
     2281 tagagtccat cgcccgcgca ccgcaggagc gcgccgcgga cctgggggcg acaacacggc
     2341 gtctgctcgg ggagttgcgt ccttacgctg gcaagttcgc gctcgtcttc gggctcgtcg
     2401 tgctcggtgc gctgactcag gctggcgggc cctggctgat cggacgcgcc atagaccgct
     2461 acatcctgcg cggcgacgta gaagggctct cacggacgat gttgttgctc ctcggggtct
     2521 acgtgctcgg ggccgtcgcc tctcggggac aggtctacca ggtagggtcc atcgggcaac
     2581 gcctcctggc ttcgatgcgg gcaagg
//
LOCUS       DATAIR010000097         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_187425, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000097 DATAIR010000000
VERSION     DATAIR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187425"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..475)
                     /locus_tag="VHM69_02475"
     CDS             complement(272..475)
                     /locus_tag="VHM69_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2739286.1"
                     /translation="MTVARVTELSAVSETSFDDAIKQGVSRATSTLRGIEGAWIKDMN
                     VLIEDGNITGYKVNIEVTFVLED"
     gene            complement(588..1304)
                     /locus_tag="VHM69_02480"
     CDS             complement(588..1304)
                     /locus_tag="VHM69_02480"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2739287.1"
                     /translation="MNEMNHERFEDLKDAYVLDALPDEERQSFEEYLAAHPERQAEID
                     ELGAIAGLLAFSPHEQEPSQELRHRVMDVVEAEAAPRQTRRRSWFAGLGDYLGARGLA
                     LGAAAVLVIGLLSWNVLLQNQVQDLQGEVKDAQVQTIELQGSWADQGAHAEVAAINEN
                     RVILVAEDMPSVPDNKTCQIWVIHEDEPKPSGLFQPDADMTATAITTPIKKSDVIAVT
                     VEPAGGSKKPTSDPVLAAKL"
     gene            complement(1301..1888)
                     /locus_tag="VHM69_02485"
     CDS             complement(1301..1888)
                     /locus_tag="VHM69_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2739288.1"
                     /translation="MAELGRQYMILADEDLISLVEAGDADAFATLYDRHIRSAFSLAY
                     RMMGERQAAEDLAQDAFIKVWRNAGSYRAERGSVRTWLLSIVHNRGIDQLRSTASRRR
                     TQEKIEASAPRSQPSEAFTETWRNSQRDQVREALRSLPPEQLKILELAYFSGYTHVEI
                     AELLSLPLGTVKGRMRLGLKKIRDYFDSRDVAVPR"
     gene            2176..>2488
                     /locus_tag="VHM69_02490"
     CDS             2176..>2488
                     /locus_tag="VHM69_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2739289.1"
                     /translation="MSGVESRSPIGLDRETSRRSFLKTAAWTGAALSAMGIGGFGAAV
                     RRAEAQAAQYKSYQGLGDAAILQFAYQLELLEGTFYDEGVNAGLFKDAFLDQIAAIRD
                     HE"
BASE COUNT          437 a          766 c          757 g          528 t
ORIGIN      
        1 tacgcttcgc ctcgaccatc acgtccgcct tcgagggcgg atagccgatc gtgccagttc
       61 cagttaacta ccgggcaggc gtgcgccctg ctccttcagc gcaaggccac gagaggagat
      121 cgtggtgaaa ggcgtctctg atcgccggca ctactgtaac ctgcttccca cggaagtctc
      181 ttaccctgtg ccgtaccgtg caatcttcag tcgagaacag aaaagccccg gccggtgggc
      241 cggggcaaag cgctgcgtgg agcctccgtc gctagtcttc caggacgaag gtaacctcta
      301 tattcacctt gtagcctgtg atgttgccgt cctcgatcag gacgttcatg tctttgatcc
      361 aggccccctc gataccgcga agcgtggaag tggcccggct cacaccttgc ttgatggcgt
      421 cgtcgaagct cgtttccgag acggcgctca actccgttac cctggctaca gtcatagaca
      481 ctccttccgg ttgatccgtt agctcctttc cctagatact tactaccccc ggacgctgat
      541 cataaacacc ctctggagca ggcgcgaagg gcatgagacg cgcttcgcta gagtttggcg
      601 gccaggacgg gatcgctcgt cggcttcttc gagcctccgg ccggctcgac ggtcaccgcg
      661 atcacgtcgg acttctttat gggagtggtg atcgccgtcg cggtcatgtc cgcgtcgggt
      721 tggaagaggc cgctaggctt gggttcgtct tcgtgaatca cccagatctg gcacgtctta
      781 ttatccggca ccgagggcat gtcctcggcg accaggatca cccggttctc attgatggcg
      841 gctacttcag cgtgagcacc ctgatccgcc cacgatccct gaagctcgat cgtctggacc
      901 tgtgcgtcct ttacctcgcc ttgcaggtcc tgtacctggt tctgcagcag cacgttccag
      961 gagaggagcc cgatcacgag caccgccgca gcgccaagcg cgaggcccct tgcgccgagg
     1021 tagtccccga gtccggcaaa ccatgacctg cgtctggtct ggcgaggcgc cgcttcggct
     1081 tcgaccacgt ccatgaccct gtggcggagt tcctgagatg gttcctgttc gtgcggggag
     1141 aacgccagaa ggcccgctat ggcgccaagc tcgtctatct cggcctgccg ctcggggtgg
     1201 gccgcgaggt actcctcgaa gctctgccgt tcttcatcgg gcagggcgtc gaggacgtag
     1261 gcgtctttga ggtcttcgaa gcgctcgtgg ttcatctcgt tcatctcggc accgccacgt
     1321 ctctagagtc gaagtagtcc ctgatcttct ttagtcccaa acgcattctg cccttgaccg
     1381 tcccaagggg caggctgagt agctcggcta tctcgacgtg cgtgtagccc gagaagtatg
     1441 ccagctccag tatcttcaat tgctcgggtg gtagagacct cagggcttct ctgacctgat
     1501 ctcgctgtga gttgcgccag gtctccgtga aggcctcgct cggttgggaa cgtggcgccg
     1561 aagcctcgat tttctcctgg gtcctgcgac gactcgccgt cgaacgcagc tggtctattc
     1621 ctctgttgtg gacgatggag aggagccagg tcctgacgct tccacgctcc gcacggtagc
     1681 ttcctgcgtt gcgccacacc ttgataaaag cgtcctgcgc caggtcctcc gccgcctgcc
     1741 gctcccccat catcctgtaa gccagtgaga aggctgagcg gatgtggcgg tcgtagagcg
     1801 tggcgaatgc gtcggcgtcg cccgcctcga cgagcgagat caaatcctcg tcggcgagga
     1861 tcatgtactg tctccccaac tccgccataa cctgattcta cggcacagca cagcacccgg
     1921 atcaaccctg ttcccgaaac agggctggtg tgtctctcca tggcaaaaaa tgtccaatgt
     1981 caaagatatc tctggaacgg gatgatctga agtatttatc cctattttcc aggcaaaacg
     2041 tcggtacaca ggtcttcctc cggcccggta gggcgcagcc agggtgtgat ctgggtccgg
     2101 gcggctcccg taaggctcgg gtgaaagcgc agtgagatgg cccgttgggg ccgagaatga
     2161 gggaggagcg tgtagatgag tggagtagag agccgcagcc cgatcgggtt ggaccgggag
     2221 acctcgcggc ggagcttcct gaagaccgcg gcgtggacgg gggcggcgct ctccgcgatg
     2281 gggataggcg gcttcggtgc cgccgtccgc agggcggagg cacaggccgc gcagtacaag
     2341 tcctaccagg ggctcggcga cgcggcgatc ctgcagttcg cttaccaact ggagttgttg
     2401 gagggtacgt tctacgacga gggtgtcaac gccggtctct tcaaggacgc gttcctcgac
     2461 cagatagctg ctatccgaga ccacgaaa
//
LOCUS       DATAIR010000098         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_421528, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000098 DATAIR010000000
VERSION     DATAIR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421528"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VHM69_02495"
     CDS             complement(<1..183)
                     /locus_tag="VHM69_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX2739290.1"
                     /translation="MSHVQRPPMLPTVSGNWVALAVRGVAAVLFGLAALIWPALTLSV
                     LIILYGAYAVVDGVFAI"
     gene            884..1537
                     /locus_tag="VHM69_02500"
     CDS             884..1537
                     /locus_tag="VHM69_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739291.1"
                     /translation="MAIRVLITDDHGVVRQGLRMFLSLDPDIEVVGEAENGEEALGMA
                     RELHPDVVLMDLLMPVMNGMEATKAIRSEMPDVEVIALTSVLEDVSVTGAMRAGAMGY
                     LLKDTQAEELHRAIRGAAEGRVQLAPEAVVKLMSDMSAPENPEALTDREIEVLKLLAR
                     GKANRQIAGDLFTDEKAVGTNVSSILRKLGVRSRTQAALHAVRTGLVSVEELSEEPW"
     gene            1531..5103
                     /locus_tag="VHM69_02505"
     CDS             1531..5103
                     /locus_tag="VHM69_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2739292.1"
                     /translation="MVRLPHRKKRTDLKPLRVLIVEDSENDALLLVRELKRGGYEPDY
                     ERVYIPEAMEEALDTSEWDVIVSDYRMPRFGALDALAMAQEASFEGPFIVVSGKVGEA
                     AAVGVMKSGAYDFLTKDNLALLIPMVERGLEETRERRRRDAILEAVRFAAERFLGETT
                     SWQDNVRAVLRRLGEAAEVSRAYIFENYRGADGEIWATQPYEWVAPGVSAQIENPLLI
                     AFPYRSAGFGRWIEMLGQGELVYGHTRDFPESEQPQLRGQDILSIVVVPIFVEELWWG
                     FVGFDECIAEREWSPAEMDTLKAAASTLGAAVRRRRAEDALRESEERYSLVVEASNDG
                     IFDWDIPTGELYWNDRLYEMLALPRLEVSPTFELFVKLLHPDDRQRVLDAITAHLERG
                     VKYSENFRLRRSSGDFIYCIGRGIAQRDDKGTSIRMVGSVSDVTERTKAEEALSQSQE
                     RYRAVIEQATDGIYLLDAQTRRILQTNPSFERMLGYAADELLGMEVYDFVAHPREDVD
                     SVIRRTLKQRRRLVGGRQYLRKDGSLLDVEVGVSVISIGGKAVICTIVRDVTERKKAE
                     EKVRSSEAELRAVFEAIADAIFVYDSEGRYLKVAPSNPSLLYRPAEEMLGKTLHEVLP
                     KEPADQLLGQIRRALETRQSASLEYGLRIGDEHLWFEATISPMTDDSVVAVARDVTER
                     KIAEEALRQSERLYRTVIEQVTENICLVDVETRDIVGSNPAFRETLGYNEEELEQMTL
                     YDIVAHDQDSVDRNIWLTVEEGRTFVGQRKYRRKDGSLIDVEASGSIIVRHGRETLCV
                     VAHDVTERARMQELLEERVSTLSTIAARVTLDLPMEDMMDTLAEGVVKASTAVACVVA
                     SVDESPGVVLRPEGAFGLPEGSKTGLEASWRAGARSPTIEAFRTQQSVLVPNTREFLL
                     GNEIYVPIHHFVREAAWDTAYIVPLISRGRSLGTLTLLYLPGQEPGEEERVFLKAVAD
                     QTAVAIENARLFSEARGKAALEERQRLARELHDSVSQALYGIALGTKTAHALVKQDPD
                     RASEPLEYVLSLAEAGMAEMRALIFELRPESLEREGLVTALEKQAAALRARHKSEVET
                     DLCTEPDVPLDVKEALYRIAQEALHNTVKHARASSVKIRMLCSSEYITLDISDDGIGF
                     ETKGDFPGHLGLRSMRERAERLGGTLAVESTPGKGTRVRTWMPV"
     gene            complement(5183..>5395)
                     /locus_tag="VHM69_02510"
     CDS             complement(5183..>5395)
                     /locus_tag="VHM69_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739293.1"
                     /translation="LGRLPLPMETSMPGVFAAGDVRYGSVNRVASAVGAGAIAVQSVH
                     EHLIKARLGLDHIVAPDHGTRGQSQR"
BASE COUNT         1119 a         1612 c         1671 g          993 t
ORIGIN      
        1 gatggcgaaa accccgtcga ccacggcgta ggcaccgtac agtatgatca gaaccgagag
       61 agtaagggct ggccagatca gcgccgccag accgaacagc acggcggcga cccctcgcac
      121 ggcaagcgcc acccagttac cggataccgt gggaagcatg ggtggtcgtt gcacatggct
      181 catcttttcc tcctcgtcct taccccgcac ctgactcttg gtgatctcct tttcatctcc
      241 ttcctcgcgt gcccacccgc cgccacgcga agatgccgct cccgagcagc agcgcggcag
      301 ccatgatctc cagcgaccag ggcccgcctg cgggagatct ctgccaggca ctcccgctgc
      361 gtggcgggta ggcctttttc aacggctgcc gcggagtgct tgctacgtca ctacgctcac
      421 tccaggagcg ccgcgttgca ggcactctaa agcgaggccc ccacgctaac ctcccccgag
      481 agtgtatttc ccctagggga aaagaagcgc tgaagtcact tcgcggagta ccgaagctaa
      541 tccccgatcc taattctcct ggcgcccttt cattcacgca gggtcgtaga gctcggcttt
      601 agccatcagg ctgcatagcg cagcgcatcg gtcaggtgtt ccagatatgc ccaattccct
      661 cgcccgatca gctcgtccct ggaagtttcg tttctctgct tgccccgtgt tgcacggtat
      721 tgcgtttccg gtggtgtcga ccatccatca ccttttcctc tattcattcg agaatttgga
      781 cacgagtaac agcatcaacg agggaataga gaattggctc agatcttatg ggggctgtac
      841 aattcgttca acgctcaagg gtgaacctat agtctgagga atcatggcga taagggtctt
      901 gataaccgac gaccacggcg tggtgcgcca gggactgcgt atgttcctct cgctggaccc
      961 tgacatcgag gtggttggcg aagccgaaaa cggcgaagaa gctcttggga tggcccgaga
     1021 gctccacccg gacgttgtct tgatggacct actgatgccg gtgatgaacg gtatggaggc
     1081 caccaaagcc atccgctcgg agatgcccga tgtggaggtc atagcactca cgagcgtgct
     1141 cgaggacgtc tcggtgaccg gggcgatgcg cgccggggcg atggggtatt tgctcaagga
     1201 cacccaggcc gaagagttgc accgagccat aaggggcgcg gccgaggggc gggtccaact
     1261 tgctcccgag gcggttgtca agctgatgag cgatatgagc gcccctgaga accccgaagc
     1321 gcttaccgac cgcgagatcg aggtcctgaa gctcctcgcg cggggtaagg ccaatagaca
     1381 gatagcaggc gacctcttca cagacgagaa ggcggtcggg actaatgtct ctagcatcct
     1441 gagaaagctt ggcgtaagga gtcgcacgca ggctgccctg cacgctgtgc gcaccggtct
     1501 ggtcagcgtc gaggagctca gcgaggagcc ttggtaaggc tgccccaccg aaagaaacgt
     1561 accgacctca aaccgctgcg ggtgctgatc gtggaagact ccgagaacga cgccctgctc
     1621 ctcgtgcggg aactcaagcg tggcggctat gagcccgatt acgagcgggt gtacattccc
     1681 gaggcgatgg aagaggcgct cgacacctcg gagtgggatg taatcgtctc cgactaccgg
     1741 atgccccgct tcggggcgct cgacgctttg gcgatggcac aagaggcgag ttttgagggg
     1801 ccattcatcg tggtgtccgg caaggtcggg gaggcggcgg ccgtaggggt gatgaagtcc
     1861 ggcgcatatg actttctcac caaggataac ctggcgctcc tgatccctat ggtggagcga
     1921 ggtttggagg agacccgaga gcggcgaagg cgcgacgcca tcctggaagc ggtgcgcttt
     1981 gccgccgagc gattcctcgg cgagacgacc agttggcaag acaacgtaag ggcggtcctg
     2041 cgccggctcg gggaggccgc cgaggttagt cgagcctaca tcttcgagaa ctaccggggg
     2101 gcggacggcg agatatgggc cacgcagccc tatgagtggg tggcgccggg cgtctcggca
     2161 cagatcgaga acccgttact aatcgctttc ccataccggt ctgcgggctt cggccgatgg
     2221 atcgagatgc tcggccaggg agagctggtg tacggccaca ctcgagactt tccagagagc
     2281 gaacagcccc aactccgggg acaggacatc ctgtccatag tggtagtgcc gatcttcgtc
     2341 gaagagctgt ggtggggttt cgtcggcttc gacgaatgta tagccgagcg cgagtggtcg
     2401 ccagcggaga tggacacgct gaaggccgcc gcaagcactc tgggcgctgc cgttcggcgc
     2461 cggcgggctg aggacgccct gcgggagagc gaagagcgct acagcctggt cgtagaggcg
     2521 tccaacgacg gcatcttcga ctgggacatc cctacgggcg aactctattg gaacgaccgg
     2581 ctctacgaga tgcttgccct ccctcgccta gaggtctccc ccacattcga gctgttcgtc
     2641 aaattgcttc acccggatga ccgtcaaagg gtccttgacg ccatcacagc ccacctcgag
     2701 cgcggcgtga agtacagtga gaacttccgg ctacgacgct cgtcaggaga ttttatctac
     2761 tgtattggcc ggggcattgc tcagcgcgac gacaagggga cctctattcg tatggtcggc
     2821 tcggtgagcg acgtcaccga gcgcacgaag gcagaggagg ccctaagcca gagccaggag
     2881 cgctatcggg ccgttatcga acaggcaacg gacggtatat acctcctcga cgcccagaca
     2941 agacgcatcc tgcagacgaa cccctcgttc gagaggatgc tggggtacgc ggcggacgag
     3001 ttgctcggga tggaggtcta tgatttcgtc gcgcacccgc gcgaggacgt tgattccgta
     3061 ataaggcgca ccctcaagca gaggcgtcgc cttgtcggtg ggagacagta tcttcgcaag
     3121 gatggctccc tcctcgacgt cgaggtgggc gttagcgtga tctccatcgg cggcaaagcg
     3181 gttatatgta ctatcgtccg cgacgtcaca gagcgaaaaa aggcagagga gaaggtccgg
     3241 tcctccgagg ccgaactgcg ggctgttttc gaggcgatag ccgatgccat attcgtctac
     3301 gactccgaag gtcgctacct taaagtcgcg ccttctaacc cctcgctgct gtatagaccg
     3361 gccgaggaga tgttgggcaa gacgctgcat gaagtcctgc ccaaagaacc ggccgaccaa
     3421 cttctcggcc agatccgacg cgcactcgaa acgcggcaaa gtgcctctct cgagtacggc
     3481 ctgcgcatag gtgatgagca tctgtggttc gaggccacca tatccccgat gacggacgat
     3541 tcggtcgttg ccgtcgcccg cgacgtcacc gagcgcaaga tagccgagga agcgctgagg
     3601 caaagcgaac gtctataccg cacggtcatc gagcaggtca ccgagaacat ctgcctcgta
     3661 gacgttgaaa cccgagacat cgtaggctcc aaccctgcct tccgggaaac cctcggttac
     3721 aacgaagaag agctagagca gatgacgctt tacgacatcg tcgcccacga ccaggatagc
     3781 gtagacagga acatctggct aaccgtggag gaaggccgca ccttcgttgg ccagaggaag
     3841 taccgtcgca aggacggctc tcttatcgac gtcgaggcca gcggaagcat aatcgtgcgc
     3901 cacggaagag agactctctg cgtcgtcgcc cacgatgtca cagagcgagc gcggatgcag
     3961 gaactgctcg aggagcgcgt gtccaccctc tcgaccatcg ctgccagggt gacattggac
     4021 ctgccgatgg aagatatgat ggacaccctg gcggagggag tggtaaaggc cagcaccgcc
     4081 gtcgcctgcg tagtggcctc ggtcgacgag tcgccgggcg tggtgctccg cccggaggga
     4141 gcattcggtc tgcccgaggg ctccaagacc ggcctggaag catcctggcg agccggggcc
     4201 cgatctccca ccatagaagc cttccgcacg cagcaatccg tgctggtccc gaacactcgc
     4261 gagtttctcc tgggaaatga aatctacgtc ccgatccacc acttcgtccg cgaggccgca
     4321 tgggataccg cctacatagt accgctcatc tccagaggtc gatccctcgg caccctcaca
     4381 ctcttgtatt tgcccggtca agagcccggc gaggaagaga gggttttcct aaaggccgtc
     4441 gccgatcaga cagccgttgc gatcgagaac gcccgcctgt tctccgaagc ccgcggcaag
     4501 gcggctctcg aagagcgtca aaggcttgct agagaactgc acgactccgt ctctcaggcg
     4561 ctctacggta tagccctcgg caccaagact gcgcacgcct tggtgaagca agacccggat
     4621 cgggcatccg agccgctcga gtacgtgctc tctttggctg aagccggcat ggctgaaatg
     4681 cgggcgctaa tcttcgagct aagacccgag tccctggaaa gggaaggact ggtcaccgcc
     4741 ctggaaaagc aggcggccgc cttgcgggcg cgtcacaaaa gtgaagtgga gaccgatctc
     4801 tgcactgaac ccgatgtccc gctggatgtt aaggaagctc tctaccgcat agcccaggag
     4861 gcattgcaca acaccgtcaa gcacgcccgt gctagcagtg tgaagataag gatgctctgc
     4921 tcttcagagt acataacact cgacatctct gacgatggta taggcttcga gaccaaagga
     4981 gacttccccg gccacttggg tttgcgctcg atgcgcgaga gggctgaacg cttgggcgga
     5041 acacttgcgg tggagagtac gcccgggaag ggaaccaggg tccgaacctg gatgcccgtc
     5101 tgagaaacct gcgtcaagca cccgcccgtt tacagcccga gacagaatac tccatcgtac
     5161 tcggatttct ggtgacatct tcttatcgtt gtgattgccc acgcgtccca tggtcaggag
     5221 caacaatgtg gtcgagtccc agcctggctt tgatgaggtg ctcgtggacg gattgcacgg
     5281 cgatggcgcc ggctcccact gccgaggcca ctctatttac cgacccgtac cttacgtcgc
     5341 cggcggcgaa gacgccgggc atgctcgtct ccattggcag gggaaggcgt ccgag
//
LOCUS       DATAIR010000099         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_98691, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000099 DATAIR010000000
VERSION     DATAIR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98691"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..895)
                     /locus_tag="VHM69_02515"
     CDS             complement(197..895)
                     /locus_tag="VHM69_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2739294.1"
                     /translation="MMENGRVALVSGGNRGIGLEVCRQLPERGITVVMGSRDGEQGLA
                     AAAGLSGGVVVRQLDVADPESVERLSRSIEEEFGRLDILVNNAGISNDDGQRGVDADL
                     DRVKEALEANLFGAWRLCEMAVPLMRRNGYGRIVNVSTGLAALEDMGGGSPGYRISKT
                     ALNALTRILASELRGSGILVNAVNPGWVQTDMGGSGATRSVEEGADALVWAATLPNNG
                     PTGGFFQDGRPIPW"
     gene            complement(961..1389)
                     /locus_tag="VHM69_02520"
     CDS             complement(961..1389)
                     /locus_tag="VHM69_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2739295.1"
                     /translation="MSDTRSEIQEIVDRETKGWDTQDVDLLLSVFHPDMVWPWPKTPK
                     SHDPMEWRLVLGRYDPERWGSTWRELFDTHELVHNDRETRKIEVSEEGDGALAVVDID
                     TLWRNAEGSESHWKGRVCKVYSRVQGEWKMTMHTGVLEYS"
     gene            complement(1403..1873)
                     /locus_tag="VHM69_02525"
     CDS             complement(1403..1873)
                     /locus_tag="VHM69_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX2739296.1"
                     /translation="MDPVRGTTYVAVVGSGTATGDLYEKAREVGKLVAERGGIIVCGG
                     LSGVMEAAARGVKEAGGTSIGILPDEDRRRQNEHLTYSVATGTGQARNLAVVCSGDVV
                     IAIGGEYGTLSEIGLARKVGRHVVVLESWDIGEHVSVASSPQEAVETAFGLIGR"
     gene            complement(1861..2796)
                     /locus_tag="VHM69_02530"
     CDS             complement(1861..2796)
                     /locus_tag="VHM69_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2739297.1"
                     /translation="MSPLSLPDFPDVTTETLQEISLRHGLDTNTFSLLPQVGIFNLIY
                     VLGEDFVLRVPRDHPISAADARKESVAVPAAREAGVRTPRIIAFDDALDLLPVPYTIY
                     ERVRGETLGLLPRDPAETPEVWRELGHDLALLHSGVAGDGPAAGIQLDEMPDPRPWPD
                     ELAEEGYFTATEARWLAQWLEHLASAALTPVSRRFLHGDSQSTNVMVREGSLEYLAVL
                     DWGSSGWGDPAWDFAGVPLRVVPSMLEGYRTGAPLEDGVVARILWRHLQLSLYLLRRD
                     PQPGSSWAERPLGMLLDVMRFLLDSPGPEWSKWTP"
     gene            complement(2804..>3392)
                     /locus_tag="VHM69_02535"
     CDS             complement(2804..>3392)
                     /locus_tag="VHM69_02535"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX2739298.1"
                     /translation="LEEIENRDLANGAVLHWGRVRTTDSVTYFKKVRVADDKEIGVYP
                     LDLPDVMLETQALWVTLPPLPREVRPSFESFGGALHAGEHGMIGLLPLFAMCDRADIG
                     GLSTPVHRQSGLPTVFVYDGYPGGVGISRRGYDAFESLARDTLGVIVRCPCERGCPAC
                     IQSPKCGNWNEPLSKDGAVGLLRYLLGQTSRYPTQ"
BASE COUNT          572 a         1173 c         1013 g          634 t
ORIGIN      
        1 ccggccagag gagcacctcc tgctcgaccg ccgcaagcaa ctcgtcatcc atcctgttcc
       61 tcctctcgcg agaatttcga actccctctg ccgatcactc tctgatcctc atgctagagc
      121 ggtcgccagc ctcgcgcgcc gtacggaggt acggttttcg acctgtacca ccgtacgggt
      181 ttgggccgcg ggagggttac caggggatcg gacgcccatc ctggaagaag ccgccggtgg
      241 ggccgttatt gggtagtgtc gccgcccaga cgagggcgtc agcgccttcc tcgacggagc
      301 gagtggcgcc agagccaccc atgtccgtct gcacccaccc agggttgacc gcgttgacga
      361 ggatgccgct cccgcgcagt tcggaggcaa ggatgcgcgt cagggcgttg agcgcggtct
      421 tcgagatgcg gtagccgggt gagccgcctc ccatgtcctc aagcgccgcg agccctgtcg
      481 agacgttgac gatcctcccg tagccgttgc gccgcatgag gggaacggcc atctcgcaca
      541 ggcgccaggc cccgaacagg ttcgcctcca gcgcctcttt caccctgtcg aggtcggcat
      601 ccacgccgcg ctggccgtcg tcgttggaga tgccggcgtt gttcaccagg atgtcgagcc
      661 tcccgaactc ctcctcgatg gagcgcgaga gacgttcgac gctctcgggg tcggccacgt
      721 ccagttgcct gacgaccacg ccgccggaga gtcccgccgc cgcggccagg ccctgctcac
      781 cgtcgcgaga gcccatgacc accgtgatcc cgcgctcagg gagctgcctg cagacctcca
      841 agcctatccc cctgtttccc ccgcttacca atgcgacccg cccgttctcc atcatgtaac
      901 ctcctccaga ttcatcctca acgatactag aagaacagtg cttctatccg actcggcgcc
      961 ttacgagtac tccagcacac ccgtgtgcat ggtcatcttc cattcgcctt gcaccctgga
     1021 atacaccttg cacacccttc ccttccagtg gctctcactg ccctccgcgt tcctccagag
     1081 ggtgtctata tcgacgacgg ctagcgcccc gtcgccctcc tcggacactt cgatcttcct
     1141 ggtctctcta tcgttgtgga ccagttcgtg tgtgtcgaac agctcgcgcc acgtactccc
     1201 ccacctttca gggtcgtacc tgccgagcac gaggcgccac tccatcggat cgtgggactt
     1261 cggggtcttg ggccagggcc agaccatatc aggatggaac acgctgagca gcaggtccac
     1321 atcctgcgta tcccagccct tcgtctccct gtcgacgatc tcctgtatct ccgacctcgt
     1381 atcgctcacg tcctcttcct ctctatctac cgatgagccc gaacgccgtc tcgaccgcct
     1441 cctgaggcga cgacgccacc gatacgtgct ctcctatgtc ccagctttcg aggaccacca
     1501 cgtgccttcc gactttcctg gcgagcccga tctccgagag cgtcccgtac tcgccgccga
     1561 tggctatgac cacgtcgccg gaacacacga ccgccaggtt ccgcgcctgc cccgttcccg
     1621 tggccacaga atacgtgagg tgctcgttct gcctcctcct gtcctcgtca ggcaggatgc
     1681 ctatggacgt acctccggcc tccttaaccc cacgggccgc ggcctccatt acgccagaga
     1741 gtccgccaca gacgatgatg cctcccctct ccgcgacgag tttgcccact tcccttgcct
     1801 tctcataaag gtcgccggtg gccgtaccag acccgacgac ggccacgtac gtcgtgcctc
     1861 ttacggggtc cacttcgacc actccggtcc gggggagtcg agcaggaaac gcatgacgtc
     1921 gaggagcatc cccagcggac gctcggccca tgagcttccc ggttggggat ctcgcctcag
     1981 taaatacagc gagagttgga gatggcgcca taggatgcgg gctaccacgc cgtcttcgag
     2041 cggtgcgccc gtacggtacc cttcgagcat cgagggtacg acccggagcg gaacaccggc
     2101 gaagtcccac gcggggtcgc cccagccgga actgccccag tccagcacgg cgaggtattc
     2161 cagggagccc tcgcggacca tcacgttcgt cgactgggag tcgccgtgca ggaaacgtcg
     2221 ggataccggc gtgagcgccg cggaggccag atgttctaac cactgcgcca gccacctcgc
     2281 ttccgtggcc gtgaagtagc cttcctcagc cagctcgtcg ggccagggtc gcgggtcggg
     2341 catctcgtcc aattgaattc cggcggccgg tccgtctccg gcgaccccag agtgtagtag
     2401 ggcgaggtcg tggccgagtt ccctccatac ttcgggggtt tcggcggggt cccgtgggag
     2461 gagaccaagc gtctcgccgc ggacgcgttc gtagatcgtg taagggacgg ggaggagatc
     2521 cagcgcgtcg tcgaaggcta ttatccgggg cgttcgtacc ccagcctctc tggccgcggg
     2581 cacggcgacg gactctttgc gcgcgtcggc ggctgagatc ggatggtctc tgggtacgcg
     2641 caggacgaag tcctccccga gaacgtagat caggttgaag ataccgacct ggggcaacag
     2701 cgagaaggtg ttggtatcga ggccgtgacg aagggagatc tcctgcaacg tctccgtcgt
     2761 cacatcggga aagtcgggca aagaaagagg cgacacgatg cacctattgc gtcgggtagc
     2821 ggctcgtctg cccgagcagg tacctcagta agccgacggc cccgtccttg ctcaggggct
     2881 cgttccagtt gccgcacttg ggggactgta tgcacgccgg gcagcctctc tcgcacgggc
     2941 accgaactat gacgcctaaa gtgtcgcggg cgagcgactc gaaggcatcg tagccgcgcc
     3001 tggagatgcc gacgccgcca gggtaaccgt cgtagacgaa gaccgtgggg aggccgctct
     3061 ggcggtgtac cggtgtggag agccccccta tatctgcacg gtcgcacatc gcgaagaggg
     3121 ggaggagccc gatcatgccg tgctcccctg cgtgcagcgc gcccccgaag ctctcgaagc
     3181 tcggcctcac ttccctgggc agcggaggga gagtgaccca gagcgcctgc gtctccagca
     3241 ttacgtcagg gagatccagg gggtagaccc cgatctcctt gtcgtcagcc accctgacct
     3301 tcttgaagta ggtgacggag tccgtcgtcc ttaccctgcc ccagtggagt acggccccgt
     3361 tcgcaaggtc acgattctcg atctcctcga ga
//
LOCUS       DATAIR010000100         8303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_6422, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000100 DATAIR010000000
VERSION     DATAIR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6422"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VHM69_02540"
     CDS             <1..370
                     /locus_tag="VHM69_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2739299.1"
                     /translation="SGPGVGSTFTFTVTFEKQSLDAHSEPGIPADLAGLRALVVDDSE
                     ANRTILEKQLDSWRVKNTGVEGGEQALEELHSAAEGGEPYDLAVLDMQMPGMDGMELA
                     RRIKADPDLSRLKLVLLTSM"
     gene            479..1276
                     /locus_tag="VHM69_02545"
     CDS             479..1276
                     /locus_tag="VHM69_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2739300.1"
                     /translation="MTQGEESPLVTRHSLREQRAGARARVLVAEDNPVNQKVAVRMLE
                     SLGYVADVAADGGEALEALSANPYEAVLMDVQMPEIDGYEATREIRRREGETRRTPII
                     AMTANAMQGDREKAFEAGMDDYVSKPVRPESLGAVLKRWVPSVEDAPLDPGVLAGLRE
                     LQGDDETDLVAELAVMFLDDARSGIQALEEALENGDAHGVRQVAHMLKGSSGNMGARM
                     MSDLCAQVEDVAASEDLGRLPDILERLREEFGRVDRALGAEVHGGRG"
     gene            1273..2217
                     /locus_tag="VHM69_02550"
     CDS             1273..2217
                     /locus_tag="VHM69_02550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2739301.1"
                     /translation="MKVLIAEDDAVSRMILRRSVEKLGHECLIAEDGVKALELYRKTP
                     EVDLVISDWMMPGIDGLELCRRIRDEVRDGYTYFIFLTALGDRDHLLMGLGAGADDYL
                     SKPLDRDELQVRLISAYRVTQLHRRLAVQNRELERLNDRLFEQSREDPLTRLGNRLRL
                     HEDLEVLQARAQRYGHTYSVVLCDVDRFKAYNDHYGHLAGDEVLKKVAATIARQRRCG
                     DTAYRYGGEEFLVVLPEQSLEAATAAADRLRKAVEELRIPHETNDPPGIVTVSVGVAA
                     MSTGGPANADELFKQADAALYKAKEAGRNRVASHGSGG"
     gene            complement(2232..2627)
                     /locus_tag="VHM69_02555"
     CDS             complement(2232..2627)
                     /locus_tag="VHM69_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2739302.1"
                     /translation="MDTSDSKILVADDDRIVRRIVVAKLSGLGYDVTEAQDGQEALRL
                     LEDGYVPDLVITDSLMPRKNGLELVRGIRSSPDLASLPVIMLTSRQNERDVIEGLETG
                     LDDYVTKPFSPDELAARVRTVLWRSRRDR"
     gene            2717..3937
                     /locus_tag="VHM69_02560"
     CDS             2717..3937
                     /locus_tag="VHM69_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX2739303.1"
                     /translation="MQSLSLTLWAVVAMIVLNVVLVTLTLGIKAVRSLNVRTTEKRIK
                     KLESALDDSLATGEVHPDLINLGDRDTDLLAIQMVDYLSVLRGADRERLTRLAEDSGL
                     VRQCFSRLRARNRWRKARAAEHLGYFGGPAAVGPLKELLSYPDETVRAVAARALARIG
                     TPDAAEGLARTLNDPSELTRLRMAENLERIGPLATPPLIETLESGDPRARVLAARILG
                     NLRAGEASRALGRAMGGGALTDVRAQAALALGRIGDPDDVPALKKAAQDDEWPVRAQA
                     ANALGMIGDVSAIPTLQRLSVDREWWVRLNGSRALANIGPAGERALAEILDGEDRYAR
                     DRAAATLEERGIVRRAVQELEAPGEKGAGARAMISAMVRAGAVRYLERLARTLPDEGT
                     RLALAKTMAEAHDA"
     gene            3927..5363
                     /locus_tag="VHM69_02565"
     CDS             3927..5363
                     /locus_tag="VHM69_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2739304.1"
                     /translation="MMLSDLIVFVNYFVLCYFLAINGVYLTLYVISFVEIADYVRRET
                     FSGFSELFGSNYAPPVSVIVPAYNEEATIASSVRSFLTLHYPLHQVVVVNDGSKDRTL
                     DVLIEEFDLHESDQPVRMQLDTAPLRCVYTATHERLVVVDKENGGKSDALNAGVCAAS
                     YPLVCCMDADIILEEDALLRVARPMIESGSVAAVGGIVRVANGCEFEKGRIVEVKTPG
                     KILPGFQIVEYLRAFIASRTAWSKLNCLMIISGAFGMFRRRDLISAGGYATDTVGEDM
                     ELVTRIHRVLLENEREYRISFVPDPIAWTEVPDTLRVLGRQRDRWHRGLIDTLFRHRR
                     MIFNPRYRTAGLLGLPYFLIFELFGPVVELLGYVAFVAGLALGVLNLEFALAFLLVAV
                     GLGVLLSTAAVFLEELRLERYPRWRDLLKLTAYGILENFGYRQINTVWRVKAIVSYLR
                     KNTEWGAMERKGFEEERRSGPDTSGDAR"
     gene            5379..7667
                     /locus_tag="VHM69_02570"
     CDS             5379..7667
                     /locus_tag="VHM69_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739305.1"
                     /translation="MYQHYEARRRASRRTRAFRLILALLVAVLLATALITLLRWGKVQ
                     RPVAEEDGIKQVSTVDGAHLAVYDGTGWKTQFWNGINMGATLPGHAPGELAPTREDYL
                     RWFAQMKEMNVDVLRVYTILNPEFYDAFHDFNSTREDPLWLIQGVWSPEEELIGPDQE
                     GRDAYTPEITHAFQNEIHDAVQVVHGDADLPERPGHASGRFRSDVSEYMLGWMVGTEW
                     FPYAVKVTDDSNQGMPPYSGRYFRGTAEASPFESWLAMMLDTLAEEEMEYGWQHPVCF
                     TNWLTTDPMSHPNEANEQEDLVPLDPMHVEPTLAWKAGYFAQYHVYPYYPDFLRYEPK
                     YQKYRNSEGEIDPYAGYLHELRAHHKGIPLFVGEFGVPSSRGMAHRGPLGRDQGYHTE
                     EEQGKMNAGLLEQIRNEGFDGALLFAWTDEWFKFTWNTTDLEMPKNRRQMWHNRLTNE
                     ENFGVLASEAGPSEEETIHLDGETYDWARRSGEAGGNFFKRLAQRALGRSYGVQEEHY
                     DDFDLSVSHDETYLYLMMDKRSGNWDFNRDEVDVGFGTLGSGSVTAKPAPGLTFPDGG
                     IQFLLQMKGKGDSRMLVNSAYDQHTWLWGYRYHYIPDTASHDTSAGEFLPWNLALSRE
                     LFLPQTRERIPFQEIEVGKMREGIGDPSSPDFNSLSDWYAKDDVLEVRIPWMLLGFTD
                     PSSLKVWDYPYETSANKDGLEAADVDGIRVYPAVRNTSEGDIDPLYYTWEEWNDPNFH
                     ERKKKSFETLSKAYEDKRLRQP"
     gene            complement(7944..8132)
                     /locus_tag="VHM69_02575"
     CDS             complement(7944..8132)
                     /locus_tag="VHM69_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX2739306.1"
                     /translation="MMPYKMITQLPDGVKNNLPKHAQEIYKEAFNSAEERYGEEARAH
                     RVVWSAVEQKYEKNEKGN"
BASE COUNT         1631 a         2639 c         2622 g         1411 t
ORIGIN      
        1 gagcgggcct ggcgttggca gcaccttcac cttcaccgtg accttcgaga agcagtcctt
       61 ggacgcccat tccgagccgg gtattcccgc agatctcgcg gggctgcggg cgcttgtggt
      121 cgacgacagc gaggccaacc gtacgatcct cgagaagcag ctcgactcgt ggcgcgtaaa
      181 gaacacgggc gtcgagggtg gtgagcaggc actcgaggag ctccactccg ccgcggaggg
      241 tggcgagccc tacgacctcg cagtccttga tatgcagatg ccgggcatgg acggtatgga
      301 gctcgccagg cggatcaaag ccgaccccga cctctcccga ctcaagctgg tacttctgac
      361 ctcgatgtga cgtcacgggg atggggcaga ggccagccag tcggggatag gtgcctatct
      421 cacgaagccc ataagacaat cggagctccg cgacgtcctc gcaacgatga tgggagaggt
      481 gacacagggc gaggagtcgc ccctcgtcac ccgccacagc ctcagggaac aaagggccgg
      541 tgcacgcgcc agggtgctcg tcgcagagga caaccccgta aaccagaagg tcgccgtgcg
      601 catgctcgaa agcctcggtt acgtggccga cgtcgccgcg gacggcgggg aggcgctgga
      661 agccctctcg gccaacccat atgaggctgt acttatggac gtgcagatgc ccgagataga
      721 cggctacgag gctaccaggg agataaggcg gcgtgaagga gagactcgtc gcactccgat
      781 catcgccatg acggccaacg ccatgcaggg cgacagggag aaggccttcg aagcagggat
      841 ggacgactac gtatccaagc cagtcaggcc agagagtctt ggcgctgtac tcaagagatg
      901 ggtaccctcc gtcgaggatg cgccactcga ccccggcgtc ctcgcggggc tgcgggagct
      961 gcagggtgac gacgagacgg acctcgtggc cgagctcgcc gtcatgtttc tggacgatgc
     1021 gcgctcgggg atccaggcgc tcgaagaggc cttggagaac ggcgacgcac acggcgtacg
     1081 gcaagtggcg catatgctca aaggcagctc aggcaacatg ggcgccagaa tgatgtccga
     1141 cctctgcgcg caggtggaag acgtagccgc ctcggaggac cttggacggc tccccgacat
     1201 cctcgaacgg ctcagagagg agttcgggag ggtcgaccgg gcgctcgggg ccgaggtcca
     1261 tgggggtagg ggttgaaggt actcatcgcc gaagacgacg ccgtctcgcg tatgatcctc
     1321 aggcgctcgg tagagaagct cggtcacgag tgcctcatcg ccgaggacgg agttaaagcc
     1381 ctcgaactct acaggaagac gcccgaggtg gacctcgtca taagcgattg gatgatgccg
     1441 ggcatcgacg gtctcgagct ctgccgccgc atcagggatg aggtacgcga cggatacact
     1501 tacttcatct tcctgacggc cctcggggat agggaccacc tgctaatggg cctcggggcc
     1561 ggcgccgacg attacctctc caaaccgctg gaccgcgacg agttgcaggt gcgactgatc
     1621 tcagcctacc gtgtgacgca gcttcaccgg cgtctggccg tccagaacag agaactggag
     1681 aggcttaacg acaggctctt cgaacagtcg cgggaggacc cgctgacccg ccttggcaac
     1741 cggctacgtc tccacgagga cctcgaggtc ctgcaggcca gggcgcagcg ctacgggcat
     1801 acctactccg tagtgctatg cgacgtcgac cggttcaaag cgtataacga ccattacggc
     1861 cacctcgcgg gcgacgaggt tctgaagaag gtggcggcga ccatcgcacg ccagcgccgt
     1921 tgcggggaca cggcctaccg ctacggcggg gaggagttcc tggtcgtact gcccgagcaa
     1981 tccctcgaag cggccactgc ggcggccgac cgcctccgca aagcggtcga ggagttgcgg
     2041 ataccccacg agaccaacga ccctcctggg atagtgaccg tcagcgtcgg cgtcgccgct
     2101 atgtctacgg gaggtcccgc gaacgccgac gagttgttca agcaggccga cgccgcactc
     2161 tacaaagcca aagaagcagg ccgtaaccgc gtggcgtccc acgggagcgg cggttgatgt
     2221 cctctacgtt ctcagcggtc gcgccgcgag cgccacagca ctgtcctcac gcgggcggcc
     2281 agctcgtcgg gggagaacgg cttggtcacg taatcgtcga ggcctgtctc cagaccttcg
     2341 atgacgtccc tctcgttctg cctggaggtg agcatgatga cggggaggct agcgaggtcc
     2401 gggctactcc ttataccgcg gacgagctcg aggccgtttt tgcggggcat caggctgtcc
     2461 gtgatgacga ggtcgggtac gtatccatcc tcgagcaggc gcagcgcctc ctgcccgtcc
     2521 tgggcctcgg taacatcgta gccaaggccg gagagcttcg cgaccacgat gcgccgcacg
     2581 atgcggtcgt cgtccgccac cagaatcttc gagtcgctcg tatccaaggg tctatcgcct
     2641 cgctaagtat tcgtgtacac accatcgaag gacaacgccg gttagaaatt atagggctac
     2701 aaggaattgt atgccgatgc agagtttgag tctaacactt tgggccgtgg tggccatgat
     2761 cgtcctgaac gtggtgctcg tgacgctcac gctcgggatc aaggccgtgc gctcgctcaa
     2821 tgtgcgcacg acggagaaga ggataaagaa gcttgaatcc gcgctcgacg actcccttgc
     2881 gacgggcgag gtccaccccg acctgataaa cctcggcgac agggacactg acctgctcgc
     2941 gatccagatg gtcgattacc tctccgtgct gcgcggggct gacagggaac gtctcaccag
     3001 gctcgcggag gattcgggtt tggtcaggca atgtttttcc aggctgcgcg cccgcaaccg
     3061 ctggcgcaag gcccgcgccg ccgagcacct cgggtacttc ggcggtccag cagccgtagg
     3121 ccctttgaag gagttgctct cgtacccgga cgagaccgtt agggcggtgg ccgcgagggc
     3181 gctggcccgt atagggaccc ccgatgccgc cgagggcctg gcgaggacgc tgaacgaccc
     3241 ctccgagctc acccgcctca ggatggccga gaacctggag cggatcggtc ccctggcgac
     3301 gccccccctg atcgaaaccc tcgaaagcgg agacccgagg gcgcgggtgc tcgcagccag
     3361 gatactcggc aacctcaggg caggggaggc aagccgggcc cttggcaggg cgatgggcgg
     3421 gggcgccctg accgacgtca gggcccaggc ggcgctggca ctcggcagga taggcgaccc
     3481 cgacgacgtg cccgccctca agaaggcagc acaagacgat gagtggccgg tccgggcgca
     3541 ggcggccaac gccctcggca tgataggcga cgtctcagcg atccctaccc ttcagaggtt
     3601 gagcgtggac agggagtggt gggtgcggct caacggaagc agggcccttg cgaacatagg
     3661 gcccgccggg gagagggctc tggcggagat cctggacgga gaagaccgct acgcgcgcga
     3721 cagggcggcg gccaccctcg aggagcgggg catcgtcagg cgggccgttc aggagctcga
     3781 agccccgggg gagaaaggcg ccggggcgcg ggcgatgatc agcgcgatgg tcagggcagg
     3841 cgccgtcaga tacctggagc gcctcgcaag gaccttgcca gacgagggca cgcgcctggc
     3901 cctggcgaag acgatggccg aggcccatga tgcttagcga cctcatagtc ttcgtcaact
     3961 acttcgtgct ctgctacttc ctggcgatca acggcgtcta cctcacgcta tacgtaatct
     4021 cttttgtcga gatagccgac tacgtcaggc gcgagacctt ctcggggttc tcggagctgt
     4081 tcggctcgaa ctacgccccg ccggtctccg tgatcgtccc ggcgtacaac gaggaggcga
     4141 ccatcgcctc gagcgtccgc tcgttcctca cccttcacta tcctctccac caggtcgtcg
     4201 tcgtcaacga cggctccaag gaccgaaccc tcgacgtctt gatagaggag ttcgacctcc
     4261 acgagtcgga ccagccggtc aggatgcaac tcgacaccgc gcccttgcgt tgcgtctaca
     4321 cggcgacgca cgagcgcctc gtcgtcgttg acaaggagaa cggcggaaag tcagacgccc
     4381 tcaacgcggg cgtctgcgcc gccagctacc cgctggtctg ctgcatggac gctgacatca
     4441 tcctcgaaga ggacgcgctg ctcagggtcg cgcggcccat gatcgaatca ggatccgtcg
     4501 cggccgttgg cgggatcgtc agggtcgcca acggctgcga gttcgagaag ggccgcatcg
     4561 tggaggtcaa gacgccgggg aagatcctgc cgggcttcca gatcgtcgag tacctgaggg
     4621 ctttcatcgc gagcaggacc gcgtggagca agctcaactg cctcatgatc atctccgggg
     4681 ctttcgggat gttcaggcgc cgcgacctca tctcagccgg cggctacgcc acggatacag
     4741 taggcgagga catggagctg gtcaccagga ttcaccgggt cctgctggag aacgagcggg
     4801 aatacaggat ctccttcgtc cccgacccca tcgcatggac cgaggtcccg gacacgctac
     4861 gcgttctcgg acgccagcgc gaccgctggc acaggggcct cattgacacg ctcttcaggc
     4921 atcgaaggat gatcttcaac ccgcgctacc gcacggcggg gctgctcggg ctgccttact
     4981 tcctgatctt cgagctcttc ggccccgtcg tcgaactgct cggctacgtg gccttcgtgg
     5041 caggtctcgc cctcggcgtc ctgaacctgg agttcgccct tgccttcctt ctcgtcgccg
     5101 tcggcctcgg ggtcctgctc tcgacggccg ccgtgttcct cgaggaactg cgcctggagc
     5161 gctaccctcg ctggcgagac ctcctgaagc tcaccgccta cggcatcctg gagaacttcg
     5221 gctacaggca gatcaacacc gtctggcggg tcaaggcaat agtctcttat ctccgcaaga
     5281 acaccgaatg gggcgccatg gaacgcaagg gcttcgaaga agaacgccgg agcgggcctg
     5341 acacttcggg cgacgctaga taaggagggg cgcaagggtt gtaccagcac tacgaggcca
     5401 ggaggcgcgc gagcaggagg acccgcgcct ttcggctcat tctggcgctg ctcgttgcgg
     5461 tccttttggc gacggccctc atcaccctct tgcggtgggg caaggtccag aggccagtcg
     5521 ccgaggagga tgggatcaaa caggtctcca ccgtcgatgg tgcccacctc gccgtctacg
     5581 acgggactgg gtggaagacc cagttctgga acgggataaa tatgggcgcc acccttcccg
     5641 gccacgcacc aggcgagctc gcgccaacca gggaggacta cctgcggtgg ttcgcgcaga
     5701 tgaaggagat gaacgtggac gtgctgcgcg tctacaccat cctcaacccc gagttctacg
     5761 acgccttcca cgatttcaac tccacccgcg aggacccgct gtggctcata cagggcgtat
     5821 ggtccccgga agaggagctc atcgggccgg atcaggaggg ccgcgatgca tacacgcccg
     5881 agataaccca cgcgttccag aatgagatcc acgacgcggt tcaggtcgtc cacggcgatg
     5941 ccgacctgcc cgagcgcccc ggacacgcaa gcgggcggtt ccgctccgac gtctccgagt
     6001 acatgctcgg ctggatggtc ggaaccgagt ggttccccta cgcggtaaag gtgacggacg
     6061 actcgaacca gggcatgcct ccctactcgg gccggtattt ccggggcacc gccgaagcct
     6121 ctccgttcga gagttggctc gccatgatgc tcgacacgct cgccgaggag gagatggagt
     6181 acggttggca gcaccccgtc tgtttcacca actggctgac caccgatccc atgagccatc
     6241 ctaacgaggc gaacgagcag gaagatctcg tccccttgga cccgatgcac gtggagccga
     6301 ccttagcgtg gaaggcgggc tacttcgccc agtaccacgt ctacccctac taccctgact
     6361 tcctccgcta cgagccgaag taccagaaat accgcaactc ggagggtgag atcgacccct
     6421 acgccggcta cctccacgag ctcagggcac accacaaagg catcccgctc ttcgtagggg
     6481 agttcggcgt cccgagctct cgtggcatgg cccaccgcgg tcccttggga cgcgaccagg
     6541 gataccacac ggaagaggag cagggaaaaa tgaacgcggg cctgttggag cagatccgga
     6601 acgaaggctt cgatggggct ctcctcttcg cttggaccga cgagtggttc aagttcacct
     6661 ggaacaccac cgacctggaa atgcccaaga acaggcgcca gatgtggcac aatcgcctga
     6721 ccaacgaaga gaatttcggt gttctggcct ccgaagccgg gcctagcgag gaggaaacca
     6781 tccacctcga cggcgagacg tacgactggg cgcggcgttc gggcgaggcc ggaggcaact
     6841 ttttcaagcg gctggcacag agggcactgg gacgctctta tggtgtccag gaagaacact
     6901 acgatgactt cgatctctcc gtctcccacg acgagaccta cctgtacctg atgatggaca
     6961 aacgtagcgg caactgggac ttcaatagag acgaggtgga cgtcggcttc ggcacgctcg
     7021 gaagcggcag cgtcaccgcg aagccagcac cgggcctcac ttttcccgat ggcggaatac
     7081 agttcctgct gcagatgaaa ggtaagggcg actcacggat gctcgtcaac agcgcctacg
     7141 atcaacacac ttggctttgg ggttaccgct accattacat ccccgatacc gccagccacg
     7201 atacgtcagc gggcgagttc ctcccgtgga acctggcgtt gagccgagag ctattcctcc
     7261 ctcagaccag ggagaggatt cctttccagg agatagaagt cggcaagatg cgtgagggca
     7321 ttggggaccc ttcaagccct gatttcaata gcctctccga ctggtacgcg aaggacgacg
     7381 tgctcgaggt ccgtatcccc tggatgctgc tcggctttac ggacccgagc agcctgaagg
     7441 tctgggacta cccttacgag acttccgcca ataaggacgg gctcgaagcg gcagatgtag
     7501 acgggatcag ggtctacccc gccgtgcgga acacgagtga gggagacata gatcccctct
     7561 actatacatg ggaggaatgg aacgacccga acttccacga gcgcaagaag aagagcttcg
     7621 agaccctcag caaggcatac gaggacaaac gactccgaca accataggcc gcacccgccc
     7681 tccggcgagg tgcgtggcct ccatgctcat catgggcgaa gcgacagccc ttctgccacc
     7741 gggcaccaac ctcacgctat atcggcgacg ccactttcca gatcacgtcc tggacaccgt
     7801 ccaatatacc tatttggctt cgacggagag tcgagagttc ctgaaggcgc cacgacgcgc
     7861 cggaccgcgg cgcacctcca ccgcaacaag cagcgcgtct acgacctggc tcccaataaa
     7921 tccagctcgg tctacgtctt gactcagttg cccttctcgt tcttctcgta tttctgctcg
     7981 acggcactcc agacgacgcg gtgggcgcgg gcctcttccc cgtatcgctc ctcggccgag
     8041 ttgaaggcct ccttgtagat ctcctgcgcg tgtttgggca ggttgttctt cacaccgtcc
     8101 ggaagctgcg tgatcatctt gtacggcatc atgctccttt cttcagagcc tccttatccg
     8161 taccctgatg tcccacgctc aggcgaagtg gtcccagccg gacaatccct cgaccggctc
     8221 actaccgcgc acgaagacga cttcgtcctt tgtgatctcc cctatcaccg tgacgggaac
     8281 ctcgacattc tctgtcagcg cgc
//
LOCUS       DATAIR010000101         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_48980, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000101 DATAIR010000000
VERSION     DATAIR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48980"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..222
                     /locus_tag="VHM69_02580"
     CDS             49..222
                     /locus_tag="VHM69_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739307.1"
                     /translation="MLPSIDMLGKTLQRSLRRVASLALKIGALRRVVLIVVAVAAGAV
                     IFSALLAVLVLRG"
     gene            422..1585
                     /locus_tag="VHM69_02585"
     CDS             422..1585
                     /locus_tag="VHM69_02585"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2739308.1"
                     /translation="MSEERTGGRRFNSATDREILNADVADSYFHRTMQILRSRGLDEV
                     PVHAEIAYKTSDPDDWFVVAGMDEVAHLLDGVDVEARAVPEGTICRPHEPVLTLSGPY
                     GAFAEHETAILGLLCQASGIATGAARCRLAAGEKPVISFGARRMHPSITPMIERSAYL
                     GGCDMVAVDLAVERLGIPATGTTPHALVIILGSTAETARAFDEVMAPEVPRTIIIDTF
                     DDEKFGALLAARAIPDSIYAIRMDTPGNRRGDFRDLMREVRWELDRNGFEHVKIFASG
                     GIDVDYILHLNPICDAYGVGGAIADAPMIDYSLDIVEVNGEDRSKRGKRGGRKRLIEL
                     EDGTRRVLPETASVPEGARDVLRPLQELYATMPDMHALRQHVLDQLGSGEFEL"
     gene            1719..>1899
                     /locus_tag="VHM69_02590"
     CDS             1719..>1899
                     /locus_tag="VHM69_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739309.1"
                     /translation="MDNSGGEARTLTCPFCSEAQLRAFGRNSLRCNECGGVLGGALLE
                     TLHRIVALPDAAGSHA"
BASE COUNT          384 a          587 c          580 g          348 t
ORIGIN      
        1 tcgacgaaac ttagactaaa cggtgagcga ataatcgaat gagcggctat gttaccgtcc
       61 attgacatgc tgggaaagac actacagcgg agcctaaggc gcgtggcttc gctcgcgctc
      121 aagatcggag ccctcagacg agtcgtgctc atcgtggtag ccgtggcggc gggtgctgtc
      181 atattctcgg ccctgctggc agtgttggtt ctccggggat aggtcccggg catggcgtga
      241 cgccgtacca gagcgtacag ccctggttca aaacgaccgg gcttgtaacg catcccgatt
      301 catgtccttg cgtttcgcct gcaaataggc acatttttga acaaggagct ttcgtattga
      361 cttgttaacg tgatttatgt ctatcatgta caggcaggca ggctagtgga aagggcgagg
      421 attgagcgaa gaaaggactg gcggcaggag gttcaactcc gccacggatc gggagatcct
      481 gaacgccgac gtcgccgact cctacttcca caggacgatg cagatcctgc gctcccgcgg
      541 cctggacgaa gtccccgtgc acgccgagat cgcgtacaag acctcagacc ccgacgactg
      601 gttcgtagtt gcgggcatgg atgaggtggc ccatctcctg gacggcgtgg acgtagaggc
      661 gcgggccgtg cctgaaggta cgatctgccg gccgcacgag ccggtcctga cgctctcggg
      721 tccatacggc gcgttcgccg aacacgagac ggcgatcctc gggctgcttt gccaggcatc
      781 cggaatcgca acgggcgcgg cccgatgcag gctcgccgcc ggtgagaagc ccgtcatctc
      841 cttcggcgcg cgccggatgc acccttctat cacaccgatg atcgagcgtt cagcctacct
      901 cggaggctgc gacatggtcg ccgttgacct cgccgtcgaa cgactcggca tcccggccac
      961 gggcacgacc cctcacgccc tcgtgattat cctcggctcg accgccgaaa ccgcccgtgc
     1021 tttcgacgag gtgatggcgc ccgaggtgcc gcgcactata atcatagaca ccttcgacga
     1081 cgagaagttc ggcgcgttgc tcgcagcgag ggcgatacct gattcgatct atgcgatccg
     1141 gatggatacg cctggcaacc gccgcggcga cttcagggac ctcatgcgtg aggtcaggtg
     1201 ggagctcgac cgcaacggct tcgagcacgt gaagatcttc gcctcaggcg gcatcgacgt
     1261 cgactacatc ctccacctga atcccatctg cgacgcctac ggtgtcggtg gcgcaatcgc
     1321 cgacgccccc atgatcgact actcgctgga catagtcgag gtaaacggcg aagaccgctc
     1381 gaaacgcggc aagcgtggcg gacgtaagcg cctgattgaa ctggaagatg ggacccgaag
     1441 ggtcctgcca gaaacggcct ccgttccgga aggagcgcgc gacgtgttgc gccccttgca
     1501 ggaactttac gccacgatgc cggacatgca cgccctgcgt cagcacgtgc tggaccagct
     1561 cggcagcgga gaattcgagc tctgaaaagt agatcccggg cactccgttc tcaccgtcta
     1621 gaccaaatag actagtaata aatcactcat ttgggcgatg tcaaaatgcc ccctttgccg
     1681 gataattagg acgtgaaaat cagtgaaggg acggtgacat ggacaactcg ggcggcgaag
     1741 cgagaaccct gacatgtccc ttctgctcgg aggcgcagct ccgcgccttc ggccgcaact
     1801 cactccgatg caacgagtgc gggggcgtcc tcggcggggc gctcctggag acactccacc
     1861 gcatcgtcgc tctccccgac gcagccggga gccacgcct
//
LOCUS       DATAIR010000102         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_233747, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000102 DATAIR010000000
VERSION     DATAIR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233747"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VHM69_02595"
     CDS             complement(<1..389)
                     /locus_tag="VHM69_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739310.1"
                     /translation="MNESRTGALKVPGATLHYEVCGSGRVLLLIPGGPADAGGFAPIR
                     DVLSDRYTVVTYDPRGLSRSPFDGEAQDTTVQTFAQDAHHLARGSPGDNAPMTDLSAE
                     FKALASYRPTHKVRFVTAASLFDGHDAA"
     gene            complement(429..1877)
                     /locus_tag="VHM69_02600"
     CDS             complement(429..1877)
                     /locus_tag="VHM69_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739311.1"
                     /translation="MTERSRWIALYVLCVGMLMIVLDVTVVNVALPSIQDDLGFSQAS
                     LAWVVNAYMIAFGGLLLLAGRFGDILGRKNVFMAGLGVFTVASLLCGAAQSQEMLVAA
                     RFVQGVGGAMTSAVILGMIVTMFPEPREQAKAIGVFAFVASAGGSVGLLAGGVLTQSI
                     NWHWIFFVNIPIGIVTAALAWRLLDREKGIGFGGGADIPGAVLITSSLLLGVYTIVKP
                     AAELGWSSGRTLGLGAVSLALLGAFIVREARASNPLVPLRIFRSRNVAGANVIQVLSV
                     AGMFGMFFLGALYLERVLGYDPLEIGLAFLPATIVMGTLSVRYSERLIMSFGARKIMI
                     PGLVLIGVALALFAQAPVDGSYVRHVFPVIVLLGFGAGVCFPALMTLAMSGATQSDAG
                     LASGLVNTTAQVGGALGLAVLATLSATRSESLIGGGETTASALTGGYHLAFLIASGLV
                     VAAIVVAVTVLESPESQEAYRDEADPAECEAA"
     gene            2042..2722
                     /locus_tag="VHM69_02605"
     CDS             2042..2722
                     /locus_tag="VHM69_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX2739312.1"
                     /translation="MPASGRRSYNDGCAAAHALDLIGERWALLVVRELLLGPKRFTDI
                     RAGVPNASPNVLAQRLRDLEASGVVRRRKLPPPAASRVYELTDWGRDLEPVIISLGRW
                     GVRSPSKPRDAELGTDSLILSLRTMFDPEATDGLSASYELRLGEDFFRAEVADGRFEI
                     ERGVADEPDAVVETDAGTLAALVYEGRQLAEALRLGDVMIEGDRQAVERLLTLFPLPE
                     PAAEAVRT"
     gene            2749..3054
                     /locus_tag="VHM69_02610"
     CDS             2749..3054
                     /locus_tag="VHM69_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2739313.1"
                     /translation="MRERNEAVFDRRKIRGLRLLQERVRRIEDLGAVAASHYREGDPE
                     KEVLKLASELDAGLIITGGQRRPWFERIFGAGFSEHVFKSADRPVSWSTKGNMKAPR"
     gene            complement(3064..3897)
                     /locus_tag="VHM69_02615"
     CDS             complement(3064..3897)
                     /locus_tag="VHM69_02615"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX2739314.1"
                     /translation="MISGKTRLLALIGQPVSHSLSPAMHNAAFMEDGLDFVYVCLDVD
                     PERLPAAVKGARALKLRGFNVTMPHKRAMVPLVDELDEGARISGAVNTVVIEDSVLHG
                     YNTDGGGMVMACNEAGIELSGRNVVLLGAGGTAAAIAVAFVNAGVGELHIANRSVEHA
                     VQLRDKLCRTGMERLAVHPLDALEEAVPHAEVVVNTTPLGMKEGDPMPVPSGYVQEGR
                     AFCDVVYRPGTETPLVRLARERGVPVVAGGRMLLYQGVLAQKLWTGREPNVKAMDRAI
                     T"
BASE COUNT          799 a         1361 c         1328 g          712 t
ORIGIN      
        1 gcggcgtcgt ggccgtcgaa cagcgatgcg gccgtgacga agcgcacctt gtgggtcggg
       61 cgatagctgg cgagcgcctt gaattcggcg gacaggtcgg tcatgggggc attgtctccg
      121 ggagagcccc gagccagatg atgcgcatcc tgcgcgaagg tctggacggt cgtgtcctga
      181 gcttcgccgt caaatgggct gcgggacaat cccctggggt cgtaggtgac caccgtatac
      241 cggtcagaaa gaacatccct gataggtgca aacccaccgg cgtccgcggg cccgcctggg
      301 atgagcagca agacacggcc gctgccgcat acctcgtagt gcagcgtcgc gccagggacc
      361 ttcagcgcgc cggtgcgaga ctcattcacg gcaacctcct ttggtctgaa gtctgtccgg
      421 ctcgcacctt acgccgcctc gcactcggcg ggatccgcct cgtcccggta cgcttcctgg
      481 ctctcggggg attccagcac cgttacggca accacgatgg ccgcgacgac gagccctgag
      541 gcgatgagga acgccagatg gtatccgccc gtcaacgcgg aggctgtggt ctccccgccg
      601 ccgatcaggc tctcgctgcg cgtcgctgac agtgtcgcga ggaccgcaag gccaagcgcg
      661 ccgcccacct gcgcggtggt attgacgagg ccggaggcga ggcccgcgtc gctctgcgta
      721 gctccagaca tcgccagcgt catcaacgcg gggaagcaga cgcctgcacc gaagccgagc
      781 aggactatca ccggaaaaac gtggcgcacg tagctgccgt ccaccggcgc ctgggcgaaa
      841 agggcgagcg cgacgcctat gagaaccaga ccggggatca ttatcttcct ggcgccgaag
      901 ctcatgatca gccgttctga gtaacgcacg gacagcgtcc ccatcacgat cgtcgctggc
      961 aggaaagcga gtccgatctc cagcgggtca tagccgagca cccgctccag atacagggct
     1021 cccaggaaaa acatcccgaa catcccggcg accgagagca cctggatcac gttggccccg
     1081 gcgacgttgc gcgagcggaa gatccggagc ggcacgagcg ggttgctcgc ccgcgcctcg
     1141 cgtacgatga acgcgccgag caacgcgagc gagacggccc cgagcccgag cgtgcgtccg
     1201 gaactccagc cgagctcggc ggcgggcttg acgatggtgt agacgccgag caacagcgag
     1261 ctggtaatga ggacggcgcc agggatgtca gctcccccgc cgaaacctat acccttctcc
     1321 ctgtcgagca accgccacgc cagggccgcc gtcacgatac cgatggggat gttcacgaag
     1381 aagatccaat gccagttgat cgactgggtc aggacacccc cggccagcag cccgacggac
     1441 ccaccagcgg aggcgacgaa cgcgaagacg cctatggcct tcgcctgctc ccgcggctcc
     1501 gggaacatgg tgacgatcat gccgagtatg acggctgagg tcatcgctcc gccgacgccc
     1561 tgtacgaaac gggccgcgac gagcatctcc tggctctgcg cggcgccaca gaggagcgag
     1621 gccactgtga agacgccgag accggccatg aagacgttct tgcgcccgag tatgtcgccg
     1681 aagcgccccg cgagcaacag caggccaccg aaggcgatca tataggcgtt gacgacccag
     1741 gcgaggctcg cctgcgagaa accgaggtcg tcctgaatcg acggcagcgc cacgttcacc
     1801 acggtcacgt cgaggacgat catgagcatc cccacacaga gcacgtagag ggcgatccaa
     1861 cggctcctct cagtcatgtg ccagctcctc acgcgtccac acctcgtcga ccgtcatact
     1921 tttagcctcc gtatttcggg gtcgtagaat catctatcta aggatactgg agaagtccta
     1981 aaatctaacc agcaaatctt ctatcaggac ttttggtggt aaaatctggt gtatggtaag
     2041 catgcccgca tcagggaggc gctcatacaa cgacggctgc gccgccgccc acgccctcga
     2101 cctcataggt gagaggtggg cgctgttagt cgtccgcgag ctcttgcttg ggcccaagcg
     2161 cttcacggat attcgggcgg gagtgcccaa tgcaagcccc aacgtcctcg cccagcgtct
     2221 gcgcgacctc gaggcatcgg gggtggtgag gcggaggaag cttcccccgc ctgcggcgtc
     2281 gagggtctac gagcttacgg attgggggag ggatctcgaa ccggtcatca tctctctcgg
     2341 tcgctggggc gtccgctcac cttcgaagcc tcgcgacgcc gaactaggca ccgattccct
     2401 gatcctctcc ctccgtacga tgttcgaccc ggaagcgacg gatgggctca gcgcaagcta
     2461 cgagctgagg ctcggtgagg acttcttcag agccgaggtc gcggatggcc gattcgagat
     2521 cgaacggggc gtcgccgacg agcccgacgc ggtcgtcgag accgacgccg gtaccctggc
     2581 tgcgctggtc tacgaaggtc gccagctggc cgaggcgctg cgcttggggg acgtcatgat
     2641 cgagggcgac agacaggcgg tggagcgctt gctcaccctc ttcccattgc cggaaccggc
     2701 cgcagaggcc gtccggacct agcatcctct ccgtatccgc ggggttcgtt gagagagcgc
     2761 aacgaagccg tgttcgaccg gagaaagata cgcgggctaa ggcttctgca ggaacgggtg
     2821 cggaggatcg aagacctcgg tgctgtcgcc gcttcgcact acagggaggg ggatcctgaa
     2881 aaagaggtac tcaagctcgc cagcgagctc gacgccggac tgatcatcac cggcggccag
     2941 aggcgtccgt ggttcgaacg gatattcgga gccggtttct cggagcacgt gttcaagagt
     3001 gctgatcgtc cggtctcgtg gtcaacgaaa gggaacatga aagctccgcg ttgatgcggt
     3061 agctcaggtg attgcccggt ccatcgcttt cacgttcggc tctcgccctg tccagagctt
     3121 ctgcgccagt acgccctggt agagcaacat cctgccgccc gcgacgacgg ggacgcctcg
     3181 ctcgcgcgcc agccggacca gaggcgtctc cgtccctgga cggtagacaa cgtcgcagaa
     3241 cgccctgccc tcttgcacgt accctgaggg cacaggcatg gggtcgcctt ccttcatccc
     3301 gagaggcgtg gtgttgacga cgacctcagc atgagggacc gcctcttcca gcgcgtcgag
     3361 gggatggacc gcgagccttt ccatacccgt ccggcacagc ttgtcccgta gctgcaccgc
     3421 gtgctcgacg ctccggttcg cgatgtgcag ctcaccgacc ccggcgttga cgaaggcgac
     3481 cgcgatggcg gcggcggtgc cccctgcgcc aaggagtacg acgttccggc ctgagagttc
     3541 gatgcccgcc tcgttgcagg ccatcaccat cccgcccccg tccgtgttgt agccatgcaa
     3601 tacggagtct tcgatcacca cggtgttcac agcccccgag atgcgtgctc cctcgtccag
     3661 ctcgtcaacg agcgggacca tcgcccgctt gtgcggcatc gtgacgttga agccgcgcaa
     3721 tttcagagcc cttgctccct ttaccgcggc gggcaggcgc tccgggtcca cgtccagaca
     3781 gacgtagacg aagtcgaggc cgtcctccat aaacgcggcg ttgtgcatcg ccggactcag
     3841 gctgtggctt acgggttgcc cgatcaaagc caacaaccgc gtcttgccgc tgatcatcca
     3901 tcccccgaca gtctctcctt cgacatgccc gccattatat agagggtggg aattgctacc
     3961 tggcctccgc tgatcgcatc catacggtta tgcaccgttc tcaccaaatg caccagaaag
     4021 actttgctgg tagcatgaga ttatgagacg agcatcggtg aacattacgg acgaattgga
     4081 gaaagcgctg gaggcgtaca ggcgggatca ggacatcccg cctcggctgg tcgatgtcgt
     4141 gcaggcggcc ctgggcgagt tcctggccgg acgaggatat ctggcttcgg atcacaggat
//
LOCUS       DATAIR010000103         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_325978, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000103 DATAIR010000000
VERSION     DATAIR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325978"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VHM69_02620"
     CDS             complement(<1..807)
                     /locus_tag="VHM69_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX2739315.1"
                     /translation="METRIPGATENLWDLRRAEGLSELEALAYRSNLLGRDRAVANYG
                     GGNTSTKSREKDHTGREVDVLWVKGSGSDLATIEANQFTGLKLDEVLPLMEREEMSDE
                     EMVAYLASCQLRPDMPRGSIETLLHAFVPYAQVDHTHPDAINMICCSEGGEELARECF
                     GEEAVWIPYIRPGFALSKQVGEAVRNNPNVKFVLLAKHGLVTWGETHEESYGRTIEAI
                     NRAAEFVASRAGEPFGGQVVEPPAPDRRETLLAEILPALRGELSSGSDESS"
     gene            complement(1007..2224)
                     /gene="rhaI"
                     /locus_tag="VHM69_02625"
     CDS             complement(1007..2224)
                     /gene="rhaI"
                     /locus_tag="VHM69_02625"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEX2739316.1"
                     /translation="MKSTDERSYDALAESLSSTGVEVASVEERLRSQKIETPSWGYGD
                     TGTRFAVFPQKGVPRDPFEKMADAAQVHGLTGVCPSVAIHIPWDRVDDYGELKGHAQS
                     LGLRIGAVNPNLFQEPEYKLGSVCHPDPSVRRKATDHLLECVEVARETGSDVLSLWFA
                     DGTNYAGQDSFTDRRHRMVESLGEVYAAMPEKMRMLLEYKPYEPAFYHTDLADWGAAL
                     TVCQRLGERAEVLVDLGHHAKEVNIEYIVALLLDEGRLGGFHFNNRKYGDDDLIVASV
                     NPFELYLIYVELVEAEERSETRIDYMIDQTHNIEPKIEAMLLSVMNLQEAYAKALLVD
                     RKTLAEARNAGDVLGAHRLLLNAYATDVRPLCAKVREDVGAAPDPIAAFTESGYAEKV
                     AQERGEGAGAGWS"
     gene            complement(2229..>3647)
                     /locus_tag="VHM69_02630"
     CDS             complement(2229..>3647)
                     /locus_tag="VHM69_02630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEX2739317.1"
                     /translation="GRVSLEEVHRFPNVPVRLPDGLHWDVLRIFREIKDGLAKAGREL
                     QIEGVGVDSWGVDFGLLDRDGALVSNPYHHRDARTDGMMDRAFGRVPREEIYRTTGIQ
                     FLPINTIYQLLAMRGSPLLAASETLLLIPDLINYWLTGERACEYTNATTTQLLDLEES
                     GWSQDLLNNLDLPSRIFAPIVPPATELGLPLPEVVEEVGAAPPVFAIASHDTASAVVA
                     VPARGDDFAYISSGTWSLVGVESPGPVVTQEAMDANFTNEGGFGNRTRFLKNVMGLWL
                     LQECRRQWAREGNEYSYEELARLAEDAPSGPLVDPDHPAFLAPGDMPSRIRRFCDETG
                     QRPPEEPAAVARCVFESLALKYRYAIEQAQSLTGRTIGMVNVVGGGSQNALLCQLTAD
                     AARLPVVAGPVEATAMGNVMVQAFAQDRVGSLEEIRAVVRDSFEASTYEPGGDADEWS
                     GLRERFSQLIDEAHALEISEGG"
BASE COUNT          624 a         1151 c         1112 g          760 t
ORIGIN      
        1 ggacgattca tctgaccccg acgagagctc cccgcgcagc gcgggcagaa tctcggcgag
       61 cagagtttct cttctatctg gtgccggcgg ctcgaccacc tgtccgccga agggctcacc
      121 ggcccggctc gcgacgaact cggcggcccg gtttatggcc tcgatggtgc ggccgtagct
      181 ctcctcatgc gtctcacccc aggtgacgag gccgtgcttg gcgagcagga cgaacttcac
      241 gttcgggttg ttccttacgg cttcgccaac ctgtttgctg agtgcgaagc cggggcggat
      301 gtaggggatc cagaccgcct cttcaccgaa gcactcgcgt gctagttcct cgccgccttc
      361 cgagcagcag atcatgttga tcgcatcagg atgcgtgtgg tcgacctgcg cataaggcac
      421 gaaggcgtgc aacagcgtct ctatggaacc cctcggcatg tccggcctga gctggcaact
      481 cgcgagatag gcgaccatct cctcgtcgct catctcctcg cgctccatga gcggcagaac
      541 ctcgtccagc ttgagcccgg taaactggtt cgcctctatg gtcgcgagat cggagcccga
      601 acccttcacc catagtacgt ccacctctcg ccctgtatgg tccttctctc tggacttggt
      661 cgacgtattg ccgccaccgt agttggcaac ggccctgtcc ctccccagaa gattggagcg
      721 gtaggcaaga gcttcgagct cggagagtcc ttccgccctc cgcaggtccc ataggttctc
      781 cgtcgctcct ggtattcgag tctccatgag tatctccctt cggtcgattt caggcttcag
      841 gcattaggtt tcaggtagtt gttctcgttt cgtcgtccgc tgacacggcg tcggacgtga
      901 gttcctcatg gcagatctcg ttggtgaggc gccgatcggt ctatcagcaa tgaactccct
      961 ccaattgatc ttcataagca ggaacctgaa gcctgaagcc ttaaatctaa ctccatcccg
     1021 cgcctgcgcc ttccccgcgc tcctgggcca ccttttctgc gtacccgctc tctgtgaagg
     1081 ctgctatggg gtcgggggcg gctccgacgt cctcgcgtac tttggcgcat agcggacgta
     1141 cgtcggtggc gtaggcgttg aggaggaggc ggtgggcgcc gagcacgtcg cccgcgtttc
     1201 gtgcttccgc cagtgtcttc cggtcaacca gcaaagcctt cgcgtaggct tcctgcaggt
     1261 tcattacgga gaggagcatg gcttcgatct ttggctcgat gttgtgcgtc tggtcgatca
     1321 tgtagtctat tcgggtttca gatctctctt cggcctcgac gagctcgacg tagatcaggt
     1381 acagttcgaa cgggttgacg ctggccacta tgaggtcgtc atccccatat ttcctgttgt
     1441 tgaagtggaa tcctccgaga cgcccctcgt cgaggaggag cgccacgatg tactctatgt
     1501 tgacctcctt ggcatggtga ccgaggtcga cgagaacctc ggccctctca ccaagcctct
     1561 gacacacggt gagggcagcg ccccagtcgg caagatcggt atggtagaag gcgggctcgt
     1621 acggcttgta ttcgaggagc atcctcatct tctctggcat ggcggcgtac acctcgccta
     1681 aagactcgac catcctgtgg cggcggtccg tgaaggagtc ctggccggcg tagttcgtgc
     1741 cgtcagcgaa ccacaaggag aggacgtcgg agcctgtttc ccttgccacc tcgacgcact
     1801 cgaggagatg atccgtggcc tttcgtcgca cgctgggatc tggatggcac acgctaccga
     1861 gtttgtactc aggttcctgg aataggttgg ggttgacggc cccgatgcga aggcccaggg
     1921 actgggcgtg tcccttcagc tcgccgtaat catctaccct gtcccagggg atatggatcg
     1981 ccaccgatgg gcatacgccg gtgagaccgt gcacctgggc tgcatcggcc atcttctcga
     2041 aaggatcgcg gggtacgccc ttttgcggga agaccgcgaa gcgcgtgccc gtatcaccgt
     2101 agccccacga cggggtttcg atcttctggg agcgcagccg ttcttccaca ctcgcaacct
     2161 ctacccccgt actggaaagg ctctcggcca gtgcatcgta ggaacgttcg tctgtgctct
     2221 tcaaatctct atcctccttc ggagatctcc agcgcatggg cttcatcgat gagctgcgag
     2281 aaccgctcgc ggagccccga ccattcgtcg gcgtcgccgc cgggctcgta cgtcgaagcc
     2341 tcgaacgagt cgcggacgac ggcccggatc tcttcgagtg aacctacccg atcctgcgcg
     2401 aaagcctgca ccatcacgtt tcccatggcg gtcgcctcga ccggcccggc caccacgggg
     2461 agcctggcag catccgcggt aagttggcac aggagcgcgt tctgagatcc tccaccgacg
     2521 acgttcacca tcccgatggt ccggcctgtc aggctctgcg cctgctcgat ggcgtacctg
     2581 tacttgagag ccaggctctc gaagacgcac cgcgccaccg ccgcgggctc ttcaggtggt
     2641 ctctgtccgg tttcatcgca gaatcgcctg attcgcgagg gcatgtctcc gggcgccagg
     2701 aacgcgggat ggtcgggatc gaccaacggg ccggacggcg cgtcttcggc gagacgggca
     2761 agttcctcgt atgagtattc attgccttcc cttgcccact gcctgcggca ctcctggaga
     2821 agccagagtc ccatcacgtt cttcaggaag cgcgtcctgt tgccaaagcc accctcgttg
     2881 gtgaagttgg catccatcgc ctcctgcgta accacgggcc ccggagactc gacgcccacg
     2941 agcgaccagg tgccgctgga gatgtaagca aagtcgtccc cccgggccgg aacggctacg
     3001 accgccgagg ctgtgtcgtg tgaggcgatc gcgaagacgg gcggtgccgc gccgacctcc
     3061 tcgaccacct cggggagtgg gagaccgagc tcggtggccg gcgggacaat tggtgcgaag
     3121 attcgggagg ggagatccaa gttattcaga aggtcctggg accagccgct ctcttcgagg
     3181 tcgaggagct gggtggttgt ggcgtttgtg tactcgcagg ccctctcgcc ggtgagccag
     3241 taattgatga ggtcggggat caggagcagc gtctctgagg ccgcaagcag cggcgagcct
     3301 cgcatggcga ggagctggta gatggtgttt atggggagga actgaatgcc cgtggtccgg
     3361 tagatctcct ccctaggcac gcgtccgaag gccctatcca tcatgccatc cgtgcgcgcg
     3421 tcacggtggt ggtaggggtt cgagaccagt gccccgtccc tgtccagaag gccgaagtca
     3481 acgccccagg aatccacgcc gacgccctcg atctgtagct cccgtccggc tttcgccaga
     3541 ccgtctttga tctcacggaa gatccgcaga acatcccagt gcaacccgtc aggtagacgg
     3601 accggcacgt tggggaagcg gtgaacctcc tcgagcgaga cgcgccc
//
LOCUS       DATAIR010000104         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_98922, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000104 DATAIR010000000
VERSION     DATAIR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98922"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="malQ"
                     /locus_tag="VHM69_02635"
     CDS             complement(<1..1073)
                     /gene="malQ"
                     /locus_tag="VHM69_02635"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2739318.1"
                     /translation="MPARIFGVTGPSAEMKPPEQTRRSAGILLHPTSLPGPHGIGELG
                     PEALRFVRFLKEAGQGIWQVLPLGPTEASPYSAYSAFAGNPLLISTDRLVEDGLLRPQ
                     AKKIAPGPVDYPAVTKAKERRLREAHGRWKPDKGFQRYREEHSGWLEDWALYAALKTK
                     FGGTPWSSWPEEYAGREKTTMARAREELKAEISFHRFVQYLFFRDWDDIKGAANAAGI
                     EVIGDVPIFVLHDSADVWANQDHFFLDEIGEPTVVAGVPPDYFSEVGQLWGNPLYRWE
                     RMRENGYSWWIARLRMALTLSDAVRVDHFRGFQAYWEVPAGEETAANGRWVEGPGAEL
                     FAALRHALGELPLVAEDLGDITP"
     gene            complement(1241..>3018)
                     /gene="glgP"
                     /locus_tag="VHM69_02640"
     CDS             complement(1241..>3018)
                     /gene="glgP"
                     /locus_tag="VHM69_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2739319.1"
                     /translation="SAEFGLHESLPIYSGGLGILAGDHLKSASDLGVPLFGVGILYAQ
                     GFFRQRLDAEGSQQEVYEPLEPENRPVAPALDVDGREVLVSVELPGRELLLKVWRAEV
                     GRVSLFLLDANLPENATEDRALTARLYGGDLSTRIAQELVLGVGGVRALRSVGIHPAV
                     FHMNEGHAAFSGLERMREFVSAGCSFEEAREEVTRRTIFTTHTPVPAGHDAFPPDLFR
                     EYTVGWPDALGTEHEDLWSLGHHDENWGPAFNMTVLAMNLSAARNAVSKLHRDVSLEM
                     WGYLFPGDEEPITHVTNGVHTWSWLAAEKGDLLNRHGGGRTWREKIEQAATWSFVDEI
                     SPGEFWETHRSVKRGMAEYVNSRLRGQEGSVALDPDALIIGFARRFATYKRATLLLTD
                     PERLRRISNDPDRPVWFIFAGKAHPADEPAKEFIRELYRRSTKDFAGHIVILEDYDMD
                     VARRLVQGVDVWLNNPRRPLEASGTSGQKASLNGAPNFSVLDGWWPEAYNGRNGWTIG
                     EEKEYSSLEEQDTVDAESLYATLESSIVPLYYDRGASGVPEGWIAMMKEAVKTVSPTF
                     STQRMVQDYVRELYVPHAMRKESRR"
BASE COUNT          516 a          952 c          986 g          564 t
ORIGIN      
        1 tcaggggtaa tgtcgccaag gtcttcggcg acgagcggca gctcccccag ggcatggcgc
       61 agggcggcga agagttccgc cccgggaccc tctacccacc ggccgttagc ggctgtctcc
      121 tcgccggcgg gaacctccca gtaagcctgg aatccccgga agtggtctac ccgcaccgcg
      181 tcgctgaggg tgagcgccat acgcaacctc gcgatccacc acgagtagcc gttctcgcgc
      241 atccgctccc agcgatagag gggattaccc cagagttgtc ccacctcgga gaagtagtca
      301 ggcggtaccc cggccacgac ggtcggctcg ccgatctcat cgaggaagaa gtggtcctgg
      361 ttggcccaaa catctgcgga gtcatgcaag acgaagatgg gtacgtcgcc tatcacttcg
      421 atgccggcag cgttggccgc ccccttgata tcgtcccagt ctcggaagaa caggtattgg
      481 acgaacctgt ggaaggagat ctccgccttc agctcctccc gcgcccgcgc catcgttgtc
      541 ttctcgcggc ccgcgtactc ctcgggccag ctgctccacg gcgttccgcc gaacttcgtc
      601 ttgagtgccg catacagcgc ccagtcctcc agccacccgc tgtgctcctc gcggtaccgc
      661 tggaagccct tgtccggctt ccagcgaccg tgggcttcgc gcagccggcg ctctttcgcc
      721 tttgtgaccg ccgggtagtc caccgggcct ggggcgatct tcttcgcctg cgggcgcagc
      781 agaccatcct ccacaaggcg gtccgtgctg ataaggagtg ggttgccggc gaaggccgag
      841 tatgcggagt agggcgatgc ctcggtggga ccgagcggga gcacctgcca gatcccctgc
      901 cccgcctcct tcaggaaccg gacgaaccgc aaggcttcgg ggcccaactc cccgatgccg
      961 tgcggaccag gcagcgaggt cgggtgtagc aggatgccgg cggagcgccg ggtctgctct
     1021 ggcggcttca tctctgcgga gggtcccgtc acgccgaaga ttctagcggg cacgccggac
     1081 ctcggccagc ggacgggacc ggtcgtggac tacttctggg tgggactcgg ccaaagggag
     1141 acattacctg agccttgagg ggcgaacaat gtagggtaca gagcccgtcc gtcatatgcc
     1201 agagaagtcc accttcgaaa tcagctcgag aggttcggta ctatcgacga gattcctttc
     1261 gcattgcgtg cggaacatag agctcacgca cgtagtcttg gaccatgcgc tgcgtgctga
     1321 aggtggggga gacggtcttc actgcctcct tcatcatcgc tatccatcct tccggtactc
     1381 ccgaggcgcc gcggtcgtag tagaggggaa cgatggagct ttcgagtgtc gcgtagaggg
     1441 actcggcgtc tacggtgtcc tgttcttcga gggaggaata ctctttctct tcgccgatgg
     1501 tccagccgtt gcggccgtta taggcctcgg gccaccagcc gtcgagcaca ctgaagttcg
     1561 gggcgccatt gagggatgcc ttctggcctg aggtgccgct cgcctccagg ggtcggcgag
     1621 ggttgttgag ccagacgtcc acgccttgca cgagacggcg cgcaacgtcc atgtcatagt
     1681 cctcgaggat cacgatgtgc ccggcgaagt ccttggtgga cctcctgtac agctccctga
     1741 tgaactcctt ggccggttcg tcggccgggt gtgccttgcc ggcgaatatg aaccaaaccg
     1801 ggcggtcggg gtcgttcgag atcctgcgca gccgctccgg gtcggtgagg aggagggtcg
     1861 cgcgcttgta tgtggcgaag cggcgggcga agccgatgat gagcgcgtcg gggtctagtg
     1921 ccacgctccc ttcttgtccg cggaggcggc tgttcacgta ctcagccatc ccgcgtttta
     1981 cggatctatg ggtctcccag aactcgccag gtgagatctc atccacgaac gaccaggtcg
     2041 ccgcttgctc gatcttctct cgccacgtcc gtccgcctcc gtggcggttg aggagatccc
     2101 ctttctcggc ggcaagccag ctccaggtgt gaacgccgtt tgtgacgtgg gttattggct
     2161 cctcatcacc cgggaagagg tatccccaca tttcgagaga aacgtcccgg tgcagtttgg
     2221 agacggcgtt gcgcgccgcc gaaaggttca tcgcgaggac ggtcatgttg aaggccggac
     2281 cccaattctc atcgtgatgg ccgaggctcc aaaggtcctc atgctcggtg cctagagcgt
     2341 caggccatcc gacggtatat tcgcggaaga ggtcgggggg gaaggcatcg tggccggccg
     2401 ggaccggggt gtgggttgta aagatcgtgc gtcgggtgac ctcctcccgg gcttcttcga
     2461 aggaacatcc cgcagagacg aactcgcgca tcctctcgag gccggagaag gcggcgtgac
     2521 cttcgttcat gtggaaaacg gccgggtgga taccgacgct gcgcagcgcc cggacgccgc
     2581 caactcccag gacaagctcc tgggcgatac gggtgcttaa gtcgccgccg tagagacggg
     2641 cggtgagcgc gcggtcttcg gtggcattct cgggtaagtt cgcgtcgagc aagaacaacg
     2701 agacgcgtcc gacctcggcc ctccagacct tcaggagcag ttcgcggcct ggcagctcga
     2761 cgctcaccaa cacctcgcga ccgtctacat caagggcggg cgcgacaggc cggttctccg
     2821 gctccagggg ctcgtaaact tcttgctgac tcccctcggc gtcgaggcgc tggcggaaga
     2881 atccctgagc atagaggatg ccgactccga agagggggac gcccagatcc gaggcgctct
     2941 tgaggtggtc gccggcgagg atgcccaatc caccggagta gatcgggagc gactcgtgca
     3001 gcccgaactc cgccgaga
//
LOCUS       DATAIR010000105         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_6663, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000105 DATAIR010000000
VERSION     DATAIR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6663"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VHM69_02645"
     CDS             complement(<1..923)
                     /locus_tag="VHM69_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX2739320.1"
                     /translation="MIRKGAPERRYAILAEGRFTDRRAKTAHGLIRYGKDEVACVIDS
                     TLAGKRVRDVLPDLGRDASIVGTLEEALELSPTSILVGLAPAGGRLPEEWMEMLRSAA
                     EAGLEIVSGLHQRLAPEFPGGNIWDVREPPESIPLFSGEGFDVEQRIALTVGTDSAIG
                     KMTATLEIERAARDAGLSPEFVPTGQTGIVIAGWGLCVDAVISDFVAGASEQLVLEAA
                     KTSPDLILVEGQGSLGHPAYSGVTLGLMHGSCPDCLILCAADPFEDVFRGVPRPSVAR
                     VARLYEEVASLIKPAPVVAVSLNTRGMDEEKA"
     gene            complement(920..1894)
                     /locus_tag="VHM69_02650"
     CDS             complement(920..1894)
                     /locus_tag="VHM69_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX2739321.1"
                     /translation="MTVHTERTFAIARSSADAFERVIFEIEEDGVVGMGEAAPTSYYG
                     QDAEGAALALESVEVMDPWDIEGTLAHNDALPPSALTALDNALHDIAARRLGVPVYRL
                     LGLGRPAPVSSYTLGIADRETTVADAKRLHAHPILKVKVGGWKDIETLRAVRESSGAE
                     LWVDANEAFSPEEALEVVAELREIGVGLIEQPVPASSGPEALRRATEAALPVPVIADE
                     SSITAGDVPRLAGSVSGVNVKLAKCGGIRRALQMIHTARAHGMLVMLGCMVETSLGIS
                     AAAQISGLVDFVDLDGAMLLADDPYSGLLYEDGRLFLPEGPGLGVESR"
     gene            1881..2063
                     /locus_tag="VHM69_02655"
     CDS             1881..2063
                     /locus_tag="VHM69_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739322.1"
                     /translation="MCTVTVFTLVFRLAPSLKDRIVASAGICVFAGKRRMRRVPTSGM
                     IAVLLKGQQRMFVSVI"
     gene            2097..2336
                     /locus_tag="VHM69_02660"
     CDS             2097..2336
                     /locus_tag="VHM69_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739323.1"
                     /translation="MAIDLSLFNSSVTTLLNHLTRHYAEDAKLETYVICARVTSAKIP
                     GGSGINWIVNPGGEELAGGLLRDVLNAVEGNGDRQ"
     gene            2370..2999
                     /locus_tag="VHM69_02665"
     CDS             2370..2999
                     /locus_tag="VHM69_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2739324.1"
                     /translation="MSIGDRELVARASRGDVEAFSKLVLAHTALVRGVTLRLLGRQEA
                     QDASQEVWVKVWANIKTFRGESAFSTWLHTITVNTCLSARRKESRRRAREVDEEMSYL
                     SEPSGGDADPEEAALDAERRGEILVALGNVRAEHRAAMVMRHMEGLSCAEISEALGVP
                     DGTVKGWASRGRSAMLAALTQGSSERIVRPHSAGQNTIPQDSKDAPGPV"
     gene            3202..3930
                     /locus_tag="VHM69_02670"
     CDS             3202..3930
                     /locus_tag="VHM69_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739325.1"
                     /translation="MASSTAEILIVEDDDVIMGTLAYNLSRSGFGVSQATSGADALRL
                     ARKLRPDLILLDIMLPGESGIEVCERIRERDQSVMIVMITAKDAEEDKVRGFEAGADD
                     YVTKPFGMKELVARINANLKRSETGGRGKIIEAGDLQLDTKNFTARAGGEQLDLRLKE
                     FELLAALASTPGELRSREELAKEVWGHAGVGSSRTIDVHIRRIRAALEVKSSYEYVHT
                     ARGLGYRFEAKPRSTATPLSERQE"
     gene            3934..>4500
                     /locus_tag="VHM69_02675"
     CDS             3934..>4500
                     /locus_tag="VHM69_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2739326.1"
                     /translation="MTLERKNGTRPVSPDELQRVNKIRRDFVANVSHELKTPATSLKL
                     LAESLEETLDEDPVQARLFAAQLKKETERLAHLITDLLDLARLESQEWVDYPTLVDVR
                     SVLMTVLARMRRVARKKDITLQWKRFGKAAEYTVYGDETQLTSMFTNLVDNAVKYTPP
                     GGKVEVVGGSEDSEIVISISDTGIGIPEG"
BASE COUNT          863 a         1421 c         1469 g          747 t
ORIGIN      
        1 gctttctctt cgtccatccc ccttgtgttg aggctgacgg cgaccacggg ggccggtttg
       61 ataagggatg cgacttcctc gtagaggcgc gccacgcggg cgaccgaagg gcgcggcacg
      121 ccccggaaga catcttcgaa agggtcggcg gcgcacagga tgaggcaatc agggcatgac
      181 ccgtgcatga ggccgagcgt cactcccgag tacgcaggat gccccaggga gccctggccc
      241 tcgacaagga tgaggtcggg cgaggttttg gccgcctcga gcacgagttg ctcggatgcc
      301 ccggcgacga agtccgagat cacggcgtcc acgcagaggc cccatcctgc gatgacgatt
      361 ccggtctgtc ccgttgggac gaactccggg gagagaccgg catccctggc tgcgcgttcg
      421 atctccaggg tcgccgtcat cttgccgatg gcgctgtccg tcccaacggt gagcgcgatc
      481 ctctgctcta cgtcgaagcc ctctccggag aagagcggga tgctttccgg cggctcccgc
      541 acgtcccaga tgttcccccc ggggaactcg ggtgcgagac gctggtggag gccgctgacg
      601 atctcgaggc ccgcctctgc cgccgaacgc agcatctcca tccactcctc aggcaaccgc
      661 cctcctgcgg gggcgagccc gacgaggatc gatgtcggcg ataactccag tgcctcttcg
      721 agcgtcccga ctatggaggc gtctcgcccg aggtcgggca ggacgtcgcg cacacgcttc
      781 cctgcgagcg tcgaatctat gacgcaggcg acctcgtctt tgccgtagcg gatcaggccg
      841 tgcgccgtct tggcgcggcg gtcggtgaag cggccttcgg ccaggatcgc gtacctccgc
      901 tccggagccc ctttccggat catcgcgact ccacccccag gcccggtccc tcaggcagga
      961 ataaacgtcc gtcttcgtag agtaatcccg agtagggatc gtcggcgagg agcatcgcgc
     1021 catcgaggtc gacgaagtcg acgaggcctg agatctgggc ggcagctgat atgccgagcg
     1081 aagtctcgac catgcagccg agcatgacga gcatcccgtg ggcgcgggct gtgtggatca
     1141 tctgaagcgc gcgccgtatc ccgccgcact tcgcgagctt gacgttgacc ccgctgacac
     1201 tccctgcgag ccgaggcacg tccccggcgg taatcgaact ctcgtcggcg atcacgggca
     1261 cgggaagggc tgcctccgtc gccctgcgca gggcctcggg acctgaagaa gcaggtacgg
     1321 gctgctcgat caatccaact cctatctccc ttagctccgc aacgacctcc agggcctcct
     1381 cgggggagaa ggcctcgttc gcgtccaccc agagctcggc cccggatgac tccctcacgg
     1441 cccgcagcgt ctcaatgtcc ttccatccac ccactttaac cttcaggatc gggtgcgcat
     1501 gcagcctctt cgcgtcggcg acggtcgtct ccctgtcggc tatgcccaac gtgtatgacg
     1561 acaccggcgc gggcctgccg agaccgagga gacggtagac gggcacgccg agcctcctgg
     1621 ctgcaatgtc gtgcagagcg ttgtcgaggg ccgtgagcgc cgacgggggc agggcgtcgt
     1681 tgtgcgcgag ggtgccttcg atgtcccatg ggtccatgac ctcgacactc tccagggcga
     1741 gtgccgcgcc ctccgcatcc tggccgtagt agcttgttgg ggcggcctcc cccatgccaa
     1801 cgacaccgtc ctcttctatc tcgaagatca cgcgctcgaa ggcgtcggcc gagctgcggg
     1861 cgatggcgaa ggtgcgctct gtgtgcaccg tcacggtctt tacgctcgtc ttcaggctgg
     1921 ccccctctct caaggaccgc attgtagcgt cggcgggcat ctgcgtcttc gccggcaagc
     1981 ggcggatgcg ccgggttccg acaagtggca tgatagcggt gttgcttaag gggcagcaac
     2041 gaatgttcgt cagtgtgatc tagcccggtg cgtccgggat gagaggacgg gtaagcgtgg
     2101 cgattgacct gtcgttgttc aacagctcgg tgacgacgct tctgaaccac ctcacccggc
     2161 actacgcaga ggatgcgaag ctggagacgt acgtgatctg cgcgagggtg acgagcgcga
     2221 aaatacctgg aggcagcggg atcaactgga tagtgaaccc cggcggcgag gaactggccg
     2281 gcgggttgtt gcgcgacgtg cttaacgccg tcgagggcaa tggagacagg cagtgaaccc
     2341 tctcaagatg tgaccgggag gatcctcggg tgagcatcgg agatcgagag ttggtcgcgc
     2401 gcgcgtcccg tggcgacgtc gaagcgttct cgaaacttgt gcttgcccac accgccctgg
     2461 tgcgcggggt gacgcttcgc ctgctcggga gacaggaagc ccaggacgcg agccaggagg
     2521 tctgggtcaa ggtctgggcg aacataaaga ccttcagggg agagagcgcc ttcagcacct
     2581 ggctgcacac gatcacggtc aacacctgcc tctccgcacg ccgcaaagag tcgcgccgga
     2641 gggcgcgcga ggtcgacgag gagatgtcgt atctctctga gccttcgggc ggagacgccg
     2701 atcccgagga ggctgcgctc gacgcggaac gccgcggaga gatcctggta gccctcggca
     2761 acgtccgcgc cgagcaccgc gccgccatgg tgatgcgcca catggagggg ctctcctgtg
     2821 cggagatatc cgaggcgctc ggcgtccccg acggtacggt caagggatgg gccagcagag
     2881 gaaggtcggc catgctcgcc gccctgacac agggctcgtc ggagcgaatc gtccgtcccc
     2941 acagcgcggg ccaaaacact attccacaag actccaaaga cgctcccggc cctgtatgac
     3001 acttcatctc gcaccttccg cacgagatga ccactaacac aatccgcaac ggccactaaa
     3061 tccttcaggt tacacgtttc agcccatgtt tgagcgtcgc tggcgttgtc tctgttaaaa
     3121 cgcggttaaa agaatgaacc ttgcgagagc agcatttata ctggctgcgg ccaattgccg
     3181 gatctagtag gagtgggagc tttggcgagc agtacggctg agatactcat agtcgaggac
     3241 gacgatgtga tcatgggcac cctcgcctac aacctctccc gcagcggctt cggggtgagc
     3301 caggcgacca gcggcgcgga cgccttgagg ctggcgagga agttgaggcc tgacctgatc
     3361 ctgctcgaca tcatgctccc aggtgaatcc ggtatcgagg tgtgcgagag gatccgcgag
     3421 cgcgaccaga gcgtgatgat cgtgatgatc acagccaaag acgcagagga agacaaggtg
     3481 cgcgggttcg aggcaggcgc cgacgactac gtgaccaagc ctttcggcat gaaggagctg
     3541 gtcgcccgca taaacgcgaa cctcaagcgc agcgagacgg gcggcagggg caagatcatc
     3601 gaggcagggg acctccagct cgacacgaag aactttacgg cccgcgccgg gggggagcag
     3661 ctcgacctgc gcctgaaaga gttcgaactg ctcgctgccc tggcctccac cccgggagag
     3721 ttgaggagcc gcgaagagct cgccaaggag gtctggggcc acgccggcgt cggctcctcg
     3781 cgcacgatag acgtgcacat acgcaggata agggcagcgc tggaagtgaa gtcctcctac
     3841 gagtacgtcc acacggcgcg gggcctgggc taccgcttcg aggcgaagcc gaggagcaca
     3901 gccacgcctc tatcagaacg ccaggagtag agtgtgacgc tggagagaaa gaacggcact
     3961 cgccccgtca gccccgacga gctccagagg gtcaacaaga taaggcgcga cttcgtcgcg
     4021 aacgtctcac acgagctcaa gactccagcg acgagcctga agctgctcgc ggagagcctg
     4081 gaggagaccc tggacgagga ccccgtccag gcgcgcctct tcgccgccca actcaagaag
     4141 gagaccgaaa ggctggccca cctcataaca gacctcctgg acctggcgcg tctcgagagc
     4201 caggagtggg tcgactatcc tacgctcgtg gacgtgcgca gcgtcctgat gaccgtgctc
     4261 gcccgcatga gacgcgtcgc ccgaaagaaa gatatcaccc tgcagtggaa acgcttcggt
     4321 aaggccgccg agtacaccgt ctacggagac gagacacaac tgacctctat gttcaccaat
     4381 ctagtggata atgccgtgaa gtacacgccg cccggtggca aggtcgaggt ggtcggaggc
     4441 tcggaggact ctgagatagt catcagcatc tccgacacgg gcatcggcat acccgagggg
//
LOCUS       DATAIR010000106         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_283638, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000106 DATAIR010000000
VERSION     DATAIR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283638"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..682
                     /gene="rfbC"
                     /locus_tag="VHM69_02680"
     CDS             122..682
                     /gene="rfbC"
                     /locus_tag="VHM69_02680"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX2739327.1"
                     /translation="MNVIETELPGVLIIEPKVFGDARGFFMETWNGARYEENGIPNHF
                     VQDNLSFSANGVLRGLHFQNPMAQGKLVSVLAGEVFDVAVDIRAGSPTFGRWTGVTLS
                     AENKRQFWVPPDFAHGFVVTGESALFSYKCTEYYAPEYDGSILWNDPEIGIEWPVESP
                     ALSEKDRAAPPLNEVPEGVLPRYTGG"
     gene            684..1076
                     /locus_tag="VHM69_02685"
     CDS             684..1076
                     /locus_tag="VHM69_02685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2739328.1"
                     /translation="MSGSFELLEGYRYVRMLTYRKSGEAVPTTVWFALVDGRAYVFTG
                     RQTGKAKRIRNNPRVNLTPSNFIGRPKVQSSVEAEARLMDEDEEAVADRAIDEKYGWQ
                     YRLYNFVLGKVASQHEHVFLELRPVGSE"
     gene            complement(1118..1957)
                     /locus_tag="VHM69_02690"
     CDS             complement(1118..1957)
                     /locus_tag="VHM69_02690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2739329.1"
                     /translation="MTFSVLIVNYASWPFTLRCIQSLRATGYGDFEIVVVDNDSVEPP
                     ELPPSVRLIRNKENVGFARAHNRGIAASKGEIVVLINPDTVVEKDFFESLAEYFEENE
                     RVGIAGPKIIDSDGQLQLSARREISALSGLLGRTSILTRLFPKSSLVKSQFPAVTDQT
                     HPTQVDWVSGACMVIRRETLREIGPLDERFFMYFEDADLCRRARAAGWLVYYLPHIEL
                     IHQTGASSRSRPKAIWLLHKSAFIYHRKHGAHGPFSLYSAMVLAGLTVRALAKLGASL
                     FAR"
     gene            complement(1954..2997)
                     /locus_tag="VHM69_02695"
     CDS             complement(1954..2997)
                     /locus_tag="VHM69_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2739330.1"
                     /translation="MSGRRGLAGQPEVLPPTVTVVIPNWNGVRLLELCLSSLRNQSFT
                     DFETLLVDNASTDGSLDFVAQTFPEVKTISLGENRGFSGAVNEGIRASGTELVALLNN
                     DTEQDPDWLRALVRAAQDHPDSGHFASKLVDFHDRRVLDGAGDAMRLSGLPYRLGHGE
                     RDGGRFDTPGYVFGACAAAALYRRDMLDEIGLFDEDFVSYCEDGDLSFRAQLAGYRCF
                     YVPGAVVYHMGSASTGGKRSPTATRLGTRNSFSLLVKNLPLSVVPHILPFFVLGQLAR
                     LLTAAATGSLRAHIEGLAGACRHLPLMLGKRAEIQKRRRTSDAEVRRLLRTSSLAATA
                     SIARRLRDGIVWR"
     gene            complement(2963..>3896)
                     /locus_tag="VHM69_02700"
     CDS             complement(2963..>3896)
                     /locus_tag="VHM69_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEX2739331.1"
                     /translation="DIEAFADIGEFIDQPVRTYSSGMRARLGFAVAINVEPDILVLDE
                     ALSAGDAAFKKKALQRMYDLRDSGTTVLFVSHSMGMVKRFCTEAVLLHKGHLVASGEP
                     EDVVEEYRTVIENAQENKESSARDRQLDYTIAHEDEEDMPDSPNGAGRRGSRPLTGEA
                     EILDLELLDERGKQVNRLPSGARLTVRVQLRYTEPVEGSTLGISLRSKRAGLEVFSTD
                     TDLEGAPLGRKEKGEQATVDFTFGVPLRSAAYIVDAVLTASQEEGSDLARTQASFRVT
                     ESDTLAPVRGLVQLPAEVRVHGLDVRQEGPGRPA"
BASE COUNT          704 a         1253 c         1243 g          696 t
ORIGIN      
        1 agatctccta ccgcaaaggc tacatcgacg ccgaagggct cgaacgcctc gcccacccga
       61 tgaggaagaa cgactacggc aggtacctgc tggagctcct gaggagcgag gagtacgtta
      121 cgtgaacgtg atcgagacgg aactccccgg cgtcctcatc atcgagccga aggtattcgg
      181 agacgccagg gggtttttca tggagacctg gaacggagcc cgctacgaag agaacggtat
      241 ccccaaccac ttcgtgcagg acaacctctc cttctcggcc aacggcgtgc tacgcggcct
      301 ccacttccag aaccccatgg cccagggtaa gctcgtctcc gtgctggccg gtgaggtctt
      361 cgacgtggcc gtagatataa gggccggctc cccgaccttc ggcaggtgga cgggcgtcac
      421 cctatcagcg gagaacaagc gccagttctg ggtacctcca gacttcgccc acggcttcgt
      481 cgtaaccggc gagagtgcgc tcttctcata caagtgcacg gagtactacg cccccgagta
      541 cgacgggtcc atcttgtgga acgatcccga gatcggtata gagtggcccg tcgagagccc
      601 ggccctctcc gaaaaggacc gagccgcccc cccgctgaat gaggtgcctg aaggggtgct
      661 acctcgctat acgggaggct gaggtgagcg gttcgttcga gttgctcgaa ggttatcgct
      721 acgtacgcat gctcacgtac cgcaagagcg gggaagcggt accaacgacg gtctggttcg
      781 cgctcgtgga tggcagggct tacgttttca ccggcaggca gacaggcaag gccaagcgca
      841 tccgcaacaa tccacgcgtg aatctgacgc caagcaactt catcggcagg cccaaagtgc
      901 agagcagcgt cgaggccgag gcccgtctca tggacgagga cgaggaggcc gtcgccgacc
      961 gggcgataga cgagaaatac ggctggcagt accgtctata taacttcgtg ctcgggaagg
     1021 ttgccagtca acacgagcac gtcttcctgg agctgcggcc cgtcggcagc gagtagtttg
     1081 ttgtggcagt atctcgggcc actcgaaatc gccgcgccta tcgcgcgaac agcgaggccc
     1141 cgagcttggc gagcgcgcgg acggtgagcc cggcgagcac catcgcgctg tagagactga
     1201 agggaccgtg ggctccgtgc ttgcggtggt agatgaaggc gctcttgtgc aggagccaga
     1261 tcgccttggg cctgctgcgg ctgctcgcgc ctgtctggtg aattaattcg atgtgcggca
     1321 ggtagtagac gagccagcct gccgcgcggg cgcggcggca gaggtcggcg tcctcgaagt
     1381 acatgaaaaa acgttcgtcg agagggccga tctctctcag agtctcccga cggatgacca
     1441 tgcacgcgcc ggagacccag tcgacctgcg tcgggtgggt ctggtccgtg acggcgggga
     1501 actggctctt caccagcgag ctcttcggga agaggcgggt cagaatggag gtgcgcccca
     1561 gcaggcctga gagggcactg atctcgcgcc gcgccgagag ctgaagttgt ccgtccgagt
     1621 cgattatctt cggccccgca atcccgaccc gctcgttctc ctcgaagtac tccgccaggc
     1681 tctcgaagaa gtccttctcc acgaccgtgt ctgggtttat gaggacgacg atttctccct
     1741 tcgaggcggc gatgcccctg ttgtgggcac gtgcgaagcc gacgttttct ttgttccgga
     1801 tcaaacgcac agacggtgga agctcgggcg gctccacact atcgttgtcg acgacgacga
     1861 tctcgaagtc tccgtagccc gtcgcacgca gagattggat gcagcgcagg gtgaaaggcc
     1921 aggaggcgta gttgacgatg agcaccgaga acgtcatcgc cacacgattc catcccgcag
     1981 ccgtcgcgcg atgctcgccg tcgcggcgag cgatgacgtc cttagcagcc tgcggacctc
     2041 ggcgtcggag gtccgcctcc tcttctgtat ctcggcccgc tttccgagca tgagcggcag
     2101 gtggcggcac gcgccggcga gtccctcgat gtgagcccgc aaagacccgg tcgccgccgc
     2161 ggtgagcagg cgcgcgagct gcccgagcac gaagaacggc agtatgtgag gcaccacgga
     2221 gaggggcagg ttcttcacaa gcaggctaaa ggagttgcgg gtgcccagcc gggtcgccgt
     2281 cgggctacgc ttgccgcctg tcgaggcgct tcccatatgg tagacgacgg cacccgggac
     2341 gtagaagcaa cgatagcccg cgagttgtgc gcggaagctc aggtccccgt cctcgcaata
     2401 agagacgaag tcctcgtcga agagcccgat ctcgtcgagc atgtccctgc ggtagagcgc
     2461 cgcggcggca cacgccccga agacgtagcc gggcgtgtcg aaccggcccc cgtcgcgctc
     2521 gccgtggccg agccgataag gaagaccgct caggcgcatg gcgtctcccg caccgtccag
     2581 cacccggcgg tcgtggaagt cgaccagctt gctcgcgaag tgacctgaat ccgggtgatc
     2641 ctgcgcggcg cgcaccagcg ctctgagcca gtccgggtcc tgctcagtat cgttgttgag
     2701 gagcgccacg agctctgtcc ctgacgccct tatcccctcg ttcacggccc ccgagaagcc
     2761 cctgttctct cccagggaga tggtcttcac ctcaggaaag gtctgggcga cgaagtcgag
     2821 ggagccgtcg gtcgaggcgt tgtctacgag tagcgtctcg aagtccgtga aagactggtt
     2881 ccgtaacgag ctcaggcaaa gctccagcag ccgcacgccg ttccagttcg ggatgacgac
     2941 cgtgacggtg ggtggcaaga cttcaggctg gccggccagg cccctcctgc cggacatcaa
     3001 gaccgtggac cctgacctcc gccggcagtt gcaccaggcc ccggacggga gcgagagtgt
     3061 cgctctcggt caccctgaac gacgcctgcg tacgggccag atccgagccc tcctcctggg
     3121 aggctgtgag gaccgcgtcc actatgtagg cggcggagcg gagcggcacc ccgaacgtga
     3181 agtccaccgt cgcctgctcc cccttctcct tccgcccgag cggggcccct tcgagatccg
     3241 tgtcagtcga gaagacttcc agcccggctc tcttgctccg cagcgagatg ccgagcgtgc
     3301 ttccctcgac gggctctgtg tagcgcagct gcaccctgac ggtcagacgc gcccccgatg
     3361 gcagcctgtt tacctgtttc cctctctcgt ccagaagttc gaggtccagg atctcagcct
     3421 ctcccgtgag agggcgagat cctcgcctcc ccgcaccgtt cggggagtcg ggcatgtcct
     3481 cttcgtcttc gtgcgcgatc gtgtagtcca gttgcctgtc cctggcgctc gattccttgt
     3541 tctcctgagc gttctcgatc acggtccggt actcctcgac aacgtcctcc ggctcgcccg
     3601 aggcgaccag gtgccccttg tggagcagca cggcctccgt gcagaacctc ttgaccatcc
     3661 ccatgctgtg cgagacgaac aggaccgttg tccccgagtc ccgcaggtca tacattcttt
     3721 gcagagcctt cttcttgaac gccgcgtcgc cggcggagag agcctcgtca aggaccagga
     3781 tgtcgggctc gacgttgatc gccacggcga agccgaggcg ggcgcgcatc ccgctcgagt
     3841 aggtcctgac aggctggtcg atgaactccc cgatgtcagc gaaggcctct atgtcg
//
LOCUS       DATAIR010000107         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_233822, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000107 DATAIR010000000
VERSION     DATAIR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233822"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..903)
                     /locus_tag="VHM69_02705"
     CDS             complement(235..903)
                     /locus_tag="VHM69_02705"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX2739332.1"
                     /translation="MGRISTMTQTFDPRVQDSILRTGSLIVITGSMFSGKTEELIRRV
                     RRALYARRSAQVLKHAIDTRSERTEIRSHNGVPHEAIAVSTSEELLGKVEETTDVVAI
                     EEAQFFDEGIVEACRRLADAGYEVIATGLDMDFRGEPFGPMPMLLAEADEVVKLRAIC
                     ARCGRDASRSQRLIDGRPAPVSAPTILVGAEESYEARCRHCHEVPEGVFQEGLFGSQT
                     PSPP"
     gene            complement(932..1645)
                     /locus_tag="VHM69_02710"
     CDS             complement(932..1645)
                     /locus_tag="VHM69_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX2739333.1"
                     /translation="MRLSARTVRWLRVMLLLAAATVFGLLYLLSAEFRSETNRALGIL
                     GRGDIAGLRDYIVSFGFWAPVASCFLMVLQALVAPIPSFLITFANGLAFGVFWGWMLS
                     LFGHVLAAAVCFWISRALGRGPVEVLVGKTGLESADRWFARWGVYAVFAGRLVPGIAF
                     DAISFAAGLTRMRFRSFIAATALGIFPQTFLYSYLGQQAPEYVFLFLTTSGLVLAGVV
                     AVAVIRYRRERRKPERNLR"
     gene            complement(1763..2005)
                     /locus_tag="VHM69_02715"
     CDS             complement(1763..2005)
                     /locus_tag="VHM69_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739334.1"
                     /translation="MEHERFAERAARRLELVADRLEEERRQYGAEGDGVMARAGELRV
                     AAHLVRAESEVVSRSEGEAPEDPMTSLGHLPLEAQA"
     gene            complement(2007..2702)
                     /locus_tag="VHM69_02720"
     CDS             complement(2007..2702)
                     /locus_tag="VHM69_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX2739335.1"
                     /translation="MRIAFIGSPGAGKSTQARRVARALPHWNTSPRLSSGDLVRAQIE
                     ADTGLGREIKSQYERGERVPDDTILSLVLPRVRRSGGWMLDNFPANVAQARALDEELE
                     ERGAGGLSRVICLEGPSEVELVERIMSGRVTSRATGEVYHFVNDPPPKPRERLDPGPF
                     ERRKDDTEEATRRHLEAYGQEADALKEHYEDRGVLSVVDARDSIDEVTGEILELLRHP
                     ETTAFYATSKGVR"
     gene            complement(2717..2896)
                     /locus_tag="VHM69_02725"
     CDS             complement(2717..2896)
                     /locus_tag="VHM69_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739336.1"
                     /translation="MKDELYSIALLNADRYEALARRAEKASDEELADFFRAMRNESRE
                     NAERAKELLAQRVAE"
     gene            complement(3361..4260)
                     /locus_tag="VHM69_02730"
     CDS             complement(3361..4260)
                     /locus_tag="VHM69_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2739337.1"
                     /translation="MRAARSLLAVPATRLKMVEKALASSADAVFLDLEDAVAPDNKAG
                     AREDVVHALKELDWRGRPRLYRANALDTPYFYRDLIEVLEEAGDALDAVMIPKVNGPE
                     DLHAVSILLDQLELARDLDPGKIKLEAQIESAEGLTNTDATARATPRLQALHFGPGDF
                     AASLRMPQTSIGVMDEWDEAYPGHRFHYAMQRIVVAARAAGLRVLDGPVADYGDEEGL
                     RRSCLIARSLGFDGKWCIHPAQIAVVNEVFSPTEKEVEWAEKVVAAYKEANAAGSGSV
                     SVDGQMVDAASIKLARNTLNLAG"
     gene            complement(4262..>4826)
                     /locus_tag="VHM69_02735"
     CDS             complement(4262..>4826)
                     /locus_tag="VHM69_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX2739338.1"
                     /translation="FEDLNLDNVIVNAAGCGSTMKEYGHLLRDDPEYAERAKAFSATV
                     RDVSEFLQELGPVAQRNPLPLTAAYHDACHLAHAQGVRRQPRQTLKQIPGMDVKEIRE
                     AEICCGSAGIYNMIEPEPASELGERKANNILKTGAQMIVTSNPGCMLQIQSSLKKMGH
                     EGLPMAHPMEVLDASLRGEPVETLLDK"
BASE COUNT          831 a         1555 c         1479 g          961 t
ORIGIN      
        1 ctgcaatgta ttcagcggac cttctccaaa tacaacacgg gcgcgctact caggttgtcc
       61 ggttgctttt gtaggctcgc tcactttgcg cttgttcatg ttgtcccaga aacggtgttc
      121 tgtgttcctg ttccgcgacg ggctgagagg acatacgtaa cgctggcggc tggccggagg
      181 tatgaatgat acacatacct atatagactc gacatggatg cgactggatt tagtctacgg
      241 aggggagggg gtctgtgaac caaaaagccc ttcctggaac acgccctcgg gaacctcgtg
      301 gcagtggcgg cacctggctt cgtagctctc ctcggccccg acaaggattg tcggcgcgga
      361 aacgggggcc ggccttccgt ctatgagccg ctgcgagcgc gaggcgtccc tgccgcagcg
      421 ggcgcagatg gccctgagct tgaccacctc gtcggcctcc gcaagcagca ttggcatcgg
      481 cccgaagggc tcacccctga agtccatgtc gaggcccgtt gctatgacct cgtagcccgc
      541 gtcagccaga cgccggcagg cttcgactat tccctcgtcg aagaactggg cctcttcgat
      601 ggctacgacg tccgtggtct cctcgacctt tccgagcagc tcttcgctcg tcgagacagc
      661 gattgcttcg tgcgggacgc cgttgtggga tcggatctcg gtccgctccg agcgggtgtc
      721 gatggcgtgc ttgaggacct gcgcgctgcg cctggcgtag agtgcgcggc ggacgcgccg
      781 gatcaactcc tccgtcttgc ccgagaacat cgagcccgta atgacgatca gagagcccgt
      841 ccgcaggatc gagtcctgta cccgtggatc aaaagtctgc gtcatggtgc ttattctacc
      901 catgagattg cccttcacag ggctctctta tctagcgcag gttcctttcg ggcttgcgtc
      961 gttccctcct gtacctgatg acagcgacgg ccaccacccc ggcgagcacc aggcccgagg
     1021 tcgtcaggaa cagaaagacg tactcgggtg cctgctgccc gaggtaggaa tagaggaaag
     1081 tctgggggaa gataccgagg gccgtggccg cgatgaagct cctgaaccgc atccgggtga
     1141 ggccggctgc aaaggagatg gcgtcgaagg cgatgcctgg cacgaggcgt ccggcgaaca
     1201 cggcgtacac cccccaccgc gcgaaccagc ggtccgccga ctcgagtccg gttttgccga
     1261 cgagcacctc gaccggaccc cgtccgagag cgcgtgagat ccagaagcac acggccgccg
     1321 caagcacgtg cccgaacagg gagagcatcc atccccagaa caccccgaaa gcgagcccgt
     1381 tggcgaaggt gatgaggaag gacgggatcg gcgcaaccag cgcctgcaac accatgagga
     1441 aacacgaagc cacaggcgcc cagaagccga acgagacgat gtaatcccgc aaccccgcta
     1501 tgtccccgcg gcccagtatc cccagagccc ggttcgtctc ggaacggaac tccgcagaca
     1561 ggagatacaa cagaccgaac accgtggccg ctgccaggag tagcatgacc cgcaaccagc
     1621 gtaccgtgcg ggcgctcagt ctcacggggc gcgccacata cggtagttgc tccggcccgt
     1681 tcaccgactc atattacacc gcctacaggc gaagataccg gataacttga ggcagatctg
     1741 ggactctcgt ggctcccctt tctcaggctt gcgcctccag cggcaggtgt ccgaggctgg
     1801 tcatgggatc ttcgggggct tccccctccg acctgcttac gacctcagac tcggcgcgta
     1861 ccaggtgcgc ggccacccga agctccccgg cccgcgccat gacaccgtca ccttcggccc
     1921 cgtattgccg gcgctcctct tcgagcctgt cagccaccaa ttcgaggcgc ctcgctgccc
     1981 gctcggcgaa ccgttcgtgt tccacgttac ctaacccctt tcgatgtggc gtagaacgct
     2041 gtcgtctcag ggtgcctgag tagttccagg atctcccccg tgacctcatc gatggaatcc
     2101 ctagcatcca cgacggagag cacacccctg tcttcgtaat gctccttcag cgcgtcggcc
     2161 tcctgcccgt aggcctccag atgccgcctc gtggcctctt cggtgtcgtc cttccggcgc
     2221 tcgaaggggc cgggatccaa cctctcccga ggcttcggcg gcggatcgtt gacgaagtgg
     2281 tagacctcgc ccgttgcccg gctcgtgacc ctcccactca tgatgcgttc gacgagttcc
     2341 acctcggagg gtccttcgag gcagatcaca cgcgatagac cccccgcgcc acgttcctcg
     2401 agctcttcgt cgagcgcacg agcctgcgcg acgttggcgg ggaagttgtc gagcatccag
     2461 ccccccgatc ttcttacgcg cggcagcacc agcgacagaa tcgtatcgtc aggcacccgc
     2521 tcgccacgtt cgtattgact tttgatctca cgcccgagcc cggtgtcggc ctctatctgc
     2581 gcccgtacga gatctcctga ggagagacgg gggctcgtgt tccagtgtgg aagagcccgc
     2641 gcgaccctcc tggcctgcgt gcttttgccc gcccctggcg agcctatgaa agctatcctc
     2701 acggtgtact cccttcttac tcagctaccc gctgggcgag cagctccttg gccctctcgg
     2761 cgttctcgcg actctcgttt cgcatagcgc ggaagaagtc ggcgagctct tcgtcgctcg
     2821 ccttctccgc ccggcgggcg agggcctcgt aacggtcggc attgagcaga gctatagagt
     2881 agagttcgtc tttcactgcg actcacctcc tgatgggcca gagcgcagaa gcccgatccc
     2941 atcccggcga tcggaccatc cgcgttgagc cccttgcggc gtatagtctg cggatccaag
     3001 ctttatcctc gcttcttttg ccatccatcc gcgtcgatgc cgcacaaaaa tagttgtggt
     3061 gcgcgtttac ctccgtatca gccgaaaacc ctgcttacgg ggacgcgcca gagcagtata
     3121 tgcgttacac atatactttc aatccacgcc ctgacccgga atgtgaacta cgtctccttt
     3181 ccggagggta ttgtgactta tgtattgatc ggcatttgat gcgagtggtt tggagaaccg
     3241 cagggtggcg gcacgtttga agggtattgg ttacagcgct tctcgtaggt agggatctcg
     3301 cagcaaggcg agagaggtca ctccgggatc cccagataga atgctgacca gagcgccggg
     3361 ctaccctgcc aggttgagcg tgttacgcgc caactttatg gaagcagcgt ccaccatctg
     3421 cccgtccacg ctcacggagc cggaacccgc ggcgttcgcc tccttgtagg ctgcgaccac
     3481 tttctctgcc cactccacct ctttctctgt gggcgagaag acctcgttga cgacggctat
     3541 ctgggccgga tggatgcacc acttgccgtc gaaaccgagg gagcgggcga tgaggcagct
     3601 ccggcgcagt ccttcctcgt ccccatagtc ggcgaccggg ccgtcgagga cgcgcaggcc
     3661 agcggctcgg gccgcgacga cgatgcgttg catggcgtag tggaaacggt ggccgggata
     3721 cgcctcgtcc cactcgtcca tcactccgat gctggtctga ggcatgcgca ggctagccgc
     3781 gaaatcgccc gggccgaagt ggagggcctg gaggcgcggg gtggcgcggg ctgtggcgtc
     3841 cgtgttggtc agtccctcgg cgctctctat ctgggcttct agtttgatct tgccggggtc
     3901 cagatccctc gccaactcca gttgatctag caggatggag acagcgtgga ggtcttccgg
     3961 gccgttcacc ttgggaatca tcacggcgtc tagtgcgtcg cccgcttctt ctaacacctc
     4021 gatcaggtcc cggtagaagt acggtgtatc cagggcgttg gcccggtaga gtcttgggcg
     4081 acctcgccag tcgagctcct tgagggcgtg gacgacgtcc tcgcgggccc cggccttgtt
     4141 gtcgggggcg acggcgtctt cgaggtcgag gaagacggcg tctgcgcttg atgcgagcgc
     4201 cttctcgacc atcttcagcc gcgtggcggg gacggcgagc agggatctgg ctgctctcac
     4261 gctacttgtc gagaagcgtc tcgaccggtt ctccacgcag ggaggcgtcg aggacctcca
     4321 tcgggtgggc catcgggagg ccttcgtggc ccatcttctt cagagaagac tggatctgga
     4381 gcatacagcc agggttcgac gtcacgatca tctgcgcgcc ggtcttgagg atgttgttgg
     4441 ccttgcgctc ccctagctcg gaggctggct cgggttcgat catgttgtag atgccggctg
     4501 atccgcagca gatctcggcc tcacggatct cctttacgtc catgcctggt atctgcttga
     4561 gggtctggcg cggctgacgg cggacgccct gcgcgtgcgc caggtggcac gcgtcgtggt
     4621 aggcggcagt aaggggcagc gggttccgct gcgcaacggg accaagctcc tgcaaaaact
     4681 ccgagacgtc gcggaccgtc gcactgaaag cctttgccct ctcggcgtac tcggggtcgt
     4741 ctcgcaggag atgaccgtac tccttcatcg tcgagccgca gccggcggcg ttcacgatca
     4801 cgttgtccag gttcaggtcc tcgaag
//
LOCUS       DATAIR010000108         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_421901, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000108 DATAIR010000000
VERSION     DATAIR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421901"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..917)
                     /locus_tag="VHM69_02740"
     CDS             complement(186..917)
                     /locus_tag="VHM69_02740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX2739339.1"
                     /translation="MGVGQKVTYFELPDQLDYPWSLSGQLETGPVMLVFYRGDWCPYC
                     NGQLASYARQFAAFEKRGTQLVGVSVDPPRNNARLVGKLQLPFPLLSDPEGELARSLG
                     LWNAEEGLAVPSIVVIDQSAEVRYLYSGSDFADRPGDDEVFAALDGLDVQMTRTTGGP
                     EIQVGAAQARERSVRPDRRVMDLDELITYYSGVFRTTVALKGRFGGWRRSGKKAFREV
                     DAYQQMVRHYIQALDETVKLTRQET"
     gene            complement(1048..2289)
                     /locus_tag="VHM69_02745"
     CDS             complement(1048..2289)
                     /locus_tag="VHM69_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2739340.1"
                     /translation="MNHVQVNTVDGRYVLVEPLGSGGMAQVYLAHDEVLDRDVALKVL
                     RSQYVRDEEFAERFRREARSAASLSHPNIVQVYDRGETEDGTCYIAMEYVSGGTLKKR
                     IEKGTLDPYTAAAVAAQIAEALQAAHERGVIHRDIKPQNVLVTSSGDLKVTDFGIARA
                     ASAVTISVTNAIFGTAGYLSPEQALGEPVSPASDLYSLGVILYEMLTGELPYTADNPM
                     AVCMKHVTEPVPQPRNLNPEIPEKMNALVVKLLAKDPADRYGSAAALLADLERMNRPA
                     SLITAEATTRLRREERQAAPRRSARPRRRRVSRGMTVAAAVALAVLGTALLGLSQEGA
                     GQGTLPRVQDVAGGLIDPPGDTPSIIAASGASATPHASASPSASASPASTFEEPVVPV
                     TPPPDDNTQTPVQSQYTGGGT"
BASE COUNT          423 a          835 c          831 g          478 t
ORIGIN      
        1 gaacccgcca gcaaccacgt gacgacgagc aggttgaaga gaagcgggac cagcaccatg
       61 taggccgcgt actggatcgc caaccgccac agcgccctga gcctgccctc ctcgagaaat
      121 ggtcccaagc agcctccgtc cttaacacgc ctctacggtc agcagatgtt ctctcggcgc
      181 gggcttcagg tctcctggcg cgttagtttg actgtctcat ccagtgcctg aatatagtgg
      241 cggaccatct gctggtaagc gtccacctcc ctgaacgcct tcttcccgga gcgcctccaa
      301 cccccgaacc tccctttgag ggccacggtg gtgcggaaga cgccgctgta gtaggtgatg
      361 agctcatcga ggtccatgac gcggcggtcg gggcggacgc tacgctcgcg cgcctgcgcc
      421 gctcctacct ggatctccgg tccgcccgtg gtgcgtgtca tctgaacgtc gagcccgtcc
      481 agcgccgcga agacctcatc gtctcccggc ctgtcagcga agtcgctgcc ggagtacagg
      541 tagcggacct cggccgactg gtcgatgacc acgatggagg gcacggccag gccttcctcc
      601 gcgttccaca ggccgaggct gcgggcgagc tctccctcgg ggtcgctgag gagggggaag
      661 ggaagctgga gcttcccgac cagcctcgcg ttattgcgcg gaggatcgac gctgacgcca
      721 acgagttgcg tccctcgctt ctcgaaggct gcgaattgcc tggcatagct ggccaattgc
      781 ccgttgcagt aggggcacca atcacccctg tagaagacga gcatcacagg tcccgtctcc
      841 agctgacctg agaggctcca tggatagtcc agttgatccg gcaactcgaa gtacgtaacc
      901 ttctgcccga cgcccaatct gcgctcctcc cctcgtggtc cctccaaggg tgcgaatata
      961 gcatcgtcgg cgggagagat ggaaccggca agtgatagtc caggacgact tcagtggcag
     1021 agacgccggg tctactgcgg gcggcgccta ggtgccgccg cctgtgtact gtgactggac
     1081 cggtgtctgg gtattgtcgt caggcggggg tgtaacgggg accacgggct cctcaaacgt
     1141 ggaagcaggc gaagccgagg cggagggcga agccgacgcg tgaggggttg cgctcgcgcc
     1201 tgacgcggcg attatcgaag gggtgtcgcc gggtgggtct ataaggccgc cggccacgtc
     1261 ttgcacgcgg ggaagagttc cctgtcccgc cccttcctgc gacaggccga gcagggccgt
     1321 accaagtaca gccagcgcca cggcggctgc gaccgtcatg ccccgggata ccctccgcct
     1381 tcgcggcctg gcgctcctgc ggggagcggc ctgccgttct tcccttcgca gtcgtgtagt
     1441 tgcctccgcc gtaattagtg aagcaggacg gttcatccgt tcgaggtcag ccaggagtgc
     1501 cgcggcgctc ccgtagcggt cggcggggtc cttggcgagg agttttacta ccagggcgtt
     1561 catcttctcg gggatttcgg ggttcagatt acgcggctgg ggtacaggct ccgtaacgtg
     1621 cttcatgcag accgccatcg ggttatcagc ggtgtaaggc agctcgcccg tgagcatctc
     1681 gtagaggatc acgccaagag agtagaggtc gctcgccggg ctgacgggct ctcccagggc
     1741 ctgctcgggc gagaggtagc cggcggtgcc gaagatggcg ttcgtcaccg agatcgtaac
     1801 ggctgaggcg gccctcgcta taccgaagtc cgtgaccttc aggtcgcccg atgaggtcac
     1861 gagtacgttc tggggcttta tgtcgcggtg gatcacccct cgctcgtggg ccgcttgcag
     1921 cgcctcggcg atctgggccg ccacggcagc cgccgtgtaa gggtcgagcg tccccttctc
     1981 gatacgcttt ttgagggtgc cgccggagac gtactccatc gcgatatagc aggtaccgtc
     2041 ctccgtctcg cccctgtcgt agacctgtac gatgttcgga tgcgataggc tcgcggcgct
     2101 gcgggcctcg cggcggaagc gctccgcgaa ctcctcgtcg cggacgtact ggctcctgag
     2161 caccttgagg gcgacgtccc tgtccagtac ttcgtcgtgg gcgaggtaga cctgagccat
     2221 gcccccgctg ccgaggggct cgaccagaac gtagcggccg tcgacggtgt tcacttgcac
     2281 gtggttcatt ctacccgccg cagacaaaag tggatacacc acgccggaag atcatcccgc
     2341 aaacacgggg tttcacgaac tccgagcgcg ctatccggtt ggagccgggt gtggccggcc
     2401 ggtccccggt ccgaccccgc tcaacgggac aggacccacc tcttcagtcc tcccgcccag
     2461 tacgtcacct tctgccacaa ggaggcctcc tcgtagcctt tctggacgac cagggggctg
     2521 ttgcccacgc tgcggccttc caccgtgacc tcgacggtgc cgagctt
//
LOCUS       DATAIR010000109        11288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_141655, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000109 DATAIR010000000
VERSION     DATAIR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141655"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHM69_02750"
     CDS             complement(<1..692)
                     /locus_tag="VHM69_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX2739341.1"
                     /translation="MIDLGKPFSEAYKDIIDALEDRVRNGVEQPDRVQFTYKGTVSTY
                     ELPRQAYAITRVTGVVGGPSERGRFFEFTPGQDFRFSNNRIIWLDGEAQPREGSRFEV
                     EYTYRERPAGLTDFNPGSVVGTLVRAVAREMKLIYDQMDEAYRRAFIDEATGAALDNV
                     VALLGVTRNPPLSAKGEVTFFLRGATDTPVVIPADTRVADESGRTFRTVEAGVILQEQ
                     DEFRVQSDGVLRV"
     gene            complement(689..1117)
                     /locus_tag="VHM69_02755"
     CDS             complement(689..1117)
                     /locus_tag="VHM69_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739342.1"
                     /translation="MTEERQRLFGDDLELYSRNETYDLRPNGAGDLGLAHGNENIIQA
                     LTLRLRVRRGDLTPLGWSDYGSRLHELIGEPNINRVHVRLMAYARSAIEEDPRVAEVS
                     EVTARVLPGERDIVRLTMDIRLIEEPNPLNMVFDFDLEGA"
     gene            complement(1128..1874)
                     /locus_tag="VHM69_02760"
     CDS             complement(1128..1874)
                     /locus_tag="VHM69_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739343.1"
                     /translation="MSAVALTIVQPQNGAVFFQGETQVRMVGQVGELPAELAGVDLFY
                     RWYSSLFPSQKDRYSINVNALGDPATPFDAPLGVGSHVISLAASDQEQETESAQNATL
                     HGGVTGGSQGEKQCVIHLFRATFVAPQPNATLNRAGSILDAEAPLQWWRTKPGTTDVY
                     ELNPDYHGINRILYRWIFEPSPADGRASRVLTPPVETLTRPSGTESRVRYAGPLPSGL
                     GTGNYTLKLRVEDKNDPNVGNQTSRAVVLT"
     gene            complement(1880..2305)
                     /locus_tag="VHM69_02765"
     CDS             complement(1880..2305)
                     /locus_tag="VHM69_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739344.1"
                     /translation="MMADLLLSGMLNLKGMLKLAGLVKANNLEVLVTGQRGAGPTHGN
                     APAPVPIPPPPAAPSDPGVGVWIFQSFNSTVKAGGKNIVAQGMCAQGNPGMATWPGMV
                     QPSIGNPGVTINNIPINVKTDMGLILPTGAPVTFSEHGQ"
     gene            complement(2302..3000)
                     /locus_tag="VHM69_02770"
     CDS             complement(2302..3000)
                     /locus_tag="VHM69_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739345.1"
                     /translation="MSRIVEIIRRVVLEELARSRGSLLGVVTTVYPHEAEDDENNYEA
                     NVRLKHEDLELRRVPIAIGHVGVAAPPRAGDLVLVQFLNGDSNQPLVTGRFYHADDRP
                     PLHKEDEILFEHRVADDTLNHLRFASDGAIYLQRDVTKPEDNSEAKTTVKIEAEGNVE
                     VKVGEKITVRLTLDDNIQVVADGLPITVDCDDMTVTAANKMTVEGDLDVNGDLKVSNG
                     STSTTISGNEITGG"
     gene            complement(2997..4040)
                     /locus_tag="VHM69_02775"
     CDS             complement(2997..4040)
                     /locus_tag="VHM69_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739346.1"
                     /translation="MPTLRPTFTLTIGSLSSSTDNPVAGPGTLVVERDMDVPADALRL
                     RLRERAGIAVDDTVTLALGHDGEEETVFTGAVETVRPAISGVVVRALGKTHDLLNLRT
                     SATYVNQSAGSIARDLIGQAGLSSGTVEDGPVLPRYVVDRAVNGYRHLKELADRLGYE
                     LYADRDGNIMFHALGPAANLDALGALGAVAGSLLGAGGESYAFGQQLLSAAAGRRGVA
                     WGTVEVGGESPMSGQGDTTEHWLTTNDADYMGSAGDAEPALLVRDPVARTKDLADRFA
                     AGRLAVAARVAREVSISVLGRPQVDIGHTISVADVPDETVNGSGYVRAIRHRFGDEAG
                     FVTEVRISVEPES"
     gene            complement(4045..7509)
                     /locus_tag="VHM69_02780"
     CDS             complement(4045..7509)
                     /locus_tag="VHM69_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX2739347.1"
                     /translation="MRRSGAVVETSSERTRLGDEAARRVEAWLLHDGQRREVFAPSGQ
                     IDAVEEAGGGRFEYAYDRRGDLTGIVEANGRSTAFEYDEARRLARVIHPDGTSTSYAY
                     AEDRLSSVAERGVVRRFEYEAEGRLTRIRHGNAGASVYRHDERGRVVEARTSTVSTTH
                     TYHQDGQIAAIRQSCDGVAVELRLEYDAAGRLAEFRLPGGDAPIRYTWDAKGRPHTVA
                     LGEEPLARFEYLDVPACSRVHLRNGVVEETGADEVDHRPVHLRIRKDRDLLFERMHAY
                     DLEGRLASDGARCFHYDAQDRLIGVDEVRTERRWRYLYDAQDNRVEAGGPDGTRKYSY
                     DADNRLVEVAGGDSFRISYDDFGRPVRKATPEGRWAYRYNDAGQLLEARHRGRSVARF
                     TYDHKGRLAAMKSAVRSERYLYGPDDQLVAVTDERGSPLRLYVWTPMGLLAEIHGSVE
                     TGSVFFHHQDHRGTRHLVTGEDGGVAARWEYDPFGVPSGPAEKHLPMFGGRVWYPEVG
                     LYYFGARWYDPGLGRFLTPDTYTGRPDDERLVHPCRPAGSQAIARSHILADWLKQPRV
                     RNRYAFCGNDPVGRIDANGHWSFGGTLLSILGAIWTLPNTIFGLLVEITCLVGEVLRW
                     LVFLVSFGNLSWETPGFDIAASGHLNAFALVFRGGWLGSFERLLGITFGNVFFVYGKW
                     DEQPYSSGPGDVYPPAYDGKVAIPKNEALYEHELRHTNQYGWFGPFFHLGLPIFGVYW
                     WDVILHGYEDAWLERDARDHAGLDEQATPPTEEPTDTTTPPAGDEAPLPPPTEILAFE
                     GKAIDKISRNPVANARVDLRGTQLRLTRKLSTFEKPDTKSKQTGSLNEGDYKVFEILR
                     NVGDADYVRVQSAGLPGGEGWVCSRSTDSHYALLYDTVEKEGAATTNAQGEYKVVVAE
                     KRLFRLRFVIENHFDAESERHSPPANDIEVELEAAPNSVKESFLIDRIDDFKNFSYKL
                     DDGRYPYELPDVSIQQAPPRQNNCVTMVEGLAIKAWKDARGDAFTWSLAKHNRMMITS
                     TDDYFSPVTETVASGLGAEIDANDLPPPWTLVQGWKTQWTGGHNFFIVDVHPDTERIL
                     TLESNSAYEMDGPGFRMLGDIDDFTDFNPGKDWWKDAKLWTWKKFKETYLHRKMARLK
                     VHDQKWVR"
     gene            complement(7506..10919)
                     /locus_tag="VHM69_02785"
     CDS             complement(7506..10919)
                     /locus_tag="VHM69_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739348.1"
                     /translation="MADGILQQLPDIQPLLVRRSAAEGQLEGFLGTLASFEPGNSSSP
                     VAPVGSVLVDLEAKLDIDTTGLSTDLSSAIDVIKNALPPGTLEYVEAIEGAYDAARDL
                     LQDNALVKEVAAGGDLKEVALAVIEEALGLFDQRIGQLGGNLIDADTLEGIRGVFASI
                     EEFRTDFESHSDDLLPFLTQHLLGVRPDLLSGPLQHLDETYAVLAPLAAEALNPARED
                     LTGSLTDLLAAVEEFDPADAAAYDELETLLGGLESAIRAMVAALTPVYGQVQTLVENH
                     AWDDVFSTYRDLLQEVTFDPPFTVDSVTDEMAEVLEEILARFYMTFGVQDLTQRIEAL
                     SAVIHDTFAASPLGQVRQTIRAFLGDIENAIDSVPTAEIQLTVEAMLERVGDEIEALG
                     ITQIKESISAALDEAREFVTENINSGLTEGVQGAVELVLANIKSLGLDTLISELTAAV
                     NKLDTLTAELQTAIEGYMEDLSSLLSQLDTLSFEPVGDQVIEQIDELKTRLEAIDPNT
                     LSDVEKLALKAALALLEEIDLEDQVISGLKQGYRAAEAEIKGLLEEITAALEQLRDTI
                     EGFDPGQVLGPVGEALDTAGAAVEKVNGQALVRPLQKQVDSLVEKLEEFSPGQVLDPL
                     QAPYDTMMETVNRLDPAQWVAPLNDLYEQIDNVIDLIDVTPLLDELDRMQRELLSNVR
                     TAIMDAFDAIDLPEPFAGFYTGMRPFVEDVTEAIFGDPDTALKQISTRVNTEVSLRRL
                     AEPLDAVFDELIGMVGEIPADDLTDAMNAVRTGLGFGLEALDPDAVIRVFREGQARLV
                     ELDPRRLLAPQALPPLKLAFEAKAEAAPPGRQSDVATVSARFDAVISLVAPGVDASLI
                     QPLIEAHDALADALRRRINALDASGAVEVYTDLRKSLDSVVPPFLRSPTPLTHDEIMA
                     GLRGMRPSVKFDRVEEVLERFLREVSPLGEALEPAFDGIFKGIREALMFLNPIDLKDA
                     VEDIYEAVRRKVRVLDPDALADSIRENFFDPLTGALQDINPATIKGEIDETYRETLDA
                     VSTTVREVLDDIAEALEEQLKAMREEIQKLLNQIETTLGTTAGRVKEVIERLEELVFV
                     ELLERLKRLIDNLGLSFDKELGRVRNAFDEMLAAIPLGSSSGASGGVAA"
     gene            complement(10922..>11288)
                     /locus_tag="VHM69_02790"
     CDS             complement(10922..>11288)
                     /locus_tag="VHM69_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739349.1"
                     /translation="FFTESVGEGYFTQVLISRLDISQRAGYLDQFDFVCDVVEYVEPP
                     EPVIADPFAALDTSLLEDAASFVDDVQNALEQVSQLADLLSTPAFGNPVESLPGIESG
                     FVQAAGEGAAVLAEIRKLF"
BASE COUNT         1811 a         3685 c         3611 g         2181 t
ORIGIN      
        1 acccgcagca cgccgtccga ctgcacacga aactcgtcct gctcctgtag gataacccct
       61 gcttcgacgg tacggaatgt gcggccgctc tcgtcggcta cgcgggtgtc cgcggggatg
      121 accacggggg tgtccgtcgc acctctcagg aagaaggtga cctctccctt tgcgctcagt
      181 ggagggttcc ttgtcacccc gagcagggcc acgacgttat ccagggctgc gcccgtggcc
      241 tcgtctataa atgccctgcg gtaggcctcg tccatctggt cgtagatcag cttcatctcc
      301 ctggcgacgg cgcggacgag cgtcccgacg acgctgccgg ggttgaagtc cgtcaggccc
      361 gcagggcgct cgcgataggt gtattccacc tcgaaccgtg agccctccct cggctgcgcc
      421 tccccgtcga gccagattat gcggttgttg ctgaaccgga aatcctggcc gggagtaaac
      481 tcgaagaacc gtcctctctc gctcggaccg ccgacgacgc cggtgacgcg cgtgatggcg
      541 tacgcctgcc ttggtagctc ataagtgctg accgtgccct tgtaggtgaa ctggacgcgg
      601 tcgggttgct cgacgccgtt gcggacgcgg tcctcgagcg cgtcgatgat gtccttgtac
      661 gcctccgaga aaggtttgcc caggtcgatc atgcgccctc cagatcgaag tcaaagacca
      721 tgttcaaagg gttgggttct tcgatcagcc ggatgtccat cgtcagacgg acgatatccc
      781 gctcgccggg cagcacgcgc gccgtgacct cgctcacttc agccaccctc gggtcctcct
      841 ctatcgcgct gcgggcgtag gccatcagcc tgacgtggac gcggttgatg ttcggctccc
      901 cgataagctc gtgcagcctg gagccgtaat ccgaccagcc gagcggggtg agatctcccc
      961 tacgcacgcg caaccgcaac gtgagcgcct gaatgatgtt ctcgttcccg tgcgccagcc
     1021 cgaggtctcc cgcgccgttg ggccgcaagt cgtacgtctc gttcctgctg tacaattcca
     1081 ggtcgtcgcc gaagagcctc tgccgttctt ctgtcatgtc accaacctca cgtcaggacc
     1141 accgcccgcg aggtctggtt tcccacgttc gggtcgttct tgtcctcgac ccgcagcttg
     1201 agggtgtagt tgcccgtgcc tagtccggat ggcaagggac ccgcgtagcg tacccgagac
     1261 tctgtcccgg acggtctggt cagcgtctcc acgggtgggg tcagaaccct cgacgcgcgt
     1321 ccgtccgccg ggctgggctc aaagatccag cggtagagaa tccgattgat cccgtgatag
     1381 tcgggattta gctcgtagac gtccgtagta ccaggcttcg tcctccacca ttgcagtgga
     1441 gcttcggcgt cgagaatgct gcccgccctg ttcagcgtgg cgttgggctg cggggcgacg
     1501 aaggtggccc tgaaaaggtg tattacgcac tgcttctcac cctgggaacc gccagtgacg
     1561 ccaccgtgaa gcgtggcgtt ctgagcgctc tctgtctctt gttcctggtc gctggcggcc
     1621 agagagatga cgtgacttcc gacacccagg ggggcatcga acggtgtggc cgggtcgcca
     1681 agcgcgttca cgttgatcga gtacctgtcc ttctgtgaag ggaacaggct ggaataccag
     1741 cggtagaaga ggtcaacccc ggcgagttcc gcgggcagtt ctcctacctg gccgaccatg
     1801 cggacctgcg tctcgccctg gaagaatacg gctccgttct ggggctggac gattgtcagt
     1861 gcgacggcgg acacagcaat cactgtccgt gctcgctgaa cgtcacaggg gcaccggtgg
     1921 gcaaaatgag gcccatgtcg gtcttcacgt tgatcgggat gttgttgatc gtaacgccgg
     1981 ggttcccgat gcttggctgc accatgccgg gccaggtagc catacccgga ttaccctgcg
     2041 cgcacatacc ctgggccacg atgttcttcc cgccggcttt caccgtcgag ttgaacgact
     2101 ggaagatcca gactcccaca ccggggtccg aaggagcggc cggtggcgga gggatgggga
     2161 cgggcgcggg ggcattgccg tgcgtcgggc cggcgccgcg ttggcctgtt acgagcactt
     2221 caaggttgtt cgccttcacc aggccggcaa gctttagcat ccccttgagg ttgagcatcc
     2281 cggacaacag caggtccgcc atcatccccc cgttatctcg ttgccgctaa tagtggtgct
     2341 cgtcgacccg ttggacacct tcaggtcgcc gttgacgtcg aggtcaccct cgacggtcat
     2401 cttgttcgcc gcggtcaccg tcatgtcgtc acagtcgacc gtgatgggaa gcccgtcggc
     2461 gacgacctgg atgttgtcgt cgagggtcag gcgcacggtt atcttctcgc ctaccttgac
     2521 ctcgacgttg ccctccgcct ctatcttgac agtggtcttc gcctcgctgt tgtcctcggg
     2581 cttggtgacg tcgcgctgaa ggtagatcgc gccatccgag gcgaagcgca ggtggttgag
     2641 ggtatcgtca gccacgcggt gctcgaagag gatctcgtcc tccttgtgca gcggcggcct
     2701 gtcgtcggcg tggtagaaac ggcccgtcac gagcggctgg ttggagtccc cgttcaggaa
     2761 ctgcacgagc acgaggtcgc ctgccctcgg cggagccgcg acgccgacgt ggccgatggc
     2821 gatgggcacg cggcgcagct cgaggtcctc gtgtttgagc cgcacgttgg cctcatagtt
     2881 gttctcgtcg tcctcggctt cgtgggggta cacggtggtc acgacgccga gcaggctccc
     2941 gcggctgcgg gcaagctcct cgagcaccac gcggcgtatg atctcgacga tgcggctcac
     3001 gactcgggct ccacggagat ccgcacctcc gtcacgaaac ccgcctcgtc gccgaaacgg
     3061 tggcgtatgg cgcggacgta cccgctgccg ttgaccgtct cgtccggcac gtcggcaacg
     3121 gagatggtgt ggccgatgtc aacctggggc ctgccgagga cggagatcga gacctcgcgc
     3181 gcgacgcggg cggcaacggc cagccggcca gcggcgaagc gatccgcaag gtccttggtg
     3241 cgcgccaccg ggtcgcgtac gagcagcgcc ggttctgcgt cacccgcgct gcccatgtag
     3301 tcggcgtcgt tcgtcgtcag ccagtgttcg gtggtgtctc cctgtcctga catcgggctc
     3361 tcgccaccga cctcgaccgt cccccacgcg acgccgcgac gccccgcggc ggcgctcagg
     3421 agttgctgcc caaaagcata gctctcgccg cccgccccga gaagagaacc ggcgacggcc
     3481 ccaagggcgc cgagcgcatc caggttcgcc gcgggtccga gagcatggaa catgatgttg
     3541 ccgtccctgt cggcgtacag ctcgtatccc aggcggtcgg cgagctcttt gagatgcctg
     3601 tagccgttca cggcgcggtc cacgacgtag cgcgggagca cgggcccatc ttcgacggtc
     3661 cccgacgaga gaccggcctg gccgatgagg tccctcgcga tgctgcccgc gctctggttg
     3721 acgtaggtgg ccgatgtgcg caggttcaac aggtcgtggg tcttgccgag cgcgcgcaca
     3781 acgacgcccg agatggctgg ccgtaccgtc tcgaccgcgc ccgtgaacac ggtctcttcc
     3841 tcgccatcgt gccccaacgc gagcgttaca gtgtcgtcga cggcgatgcc ggcacgttcc
     3901 cgcagtcgca ggcgcaacgc gtcggcggga acgtccatgt ccctctccac gacgagcgtg
     3961 ccaggacccg cgacggggtt gtcggtgctc gagctcaggc taccgatggt caacgtgaac
     4021 gtcggccgca gcgtcggcat ccgcctacct cacccacttc tggtcgtgca ccttcagccg
     4081 cgccatcttc ctgtggagat aggtctcctt gaacttcttc caggtccaga gcttcgcgtc
     4141 tttccaccag tctttgccgg ggttgaaatc cgtgaagtcg tctatgtcgc cgagcatgcg
     4201 gaaccccggt ccgtccatct cgtaagcgct gttggactcc agggtgagta tcctctccgt
     4261 gtcggggtgg acgtcgacga tgaagaagtt gtggcctcca gtccactgtg ttttccaacc
     4321 ctgcaccagc gtccacggcg gcggtagatc gttggcgtcg atctcggccc cgaggcccga
     4381 tgctacggtc tcggtcacgg gcgagaagta gtcgtcggtg gaggtgatca tcatcctgtt
     4441 gtgcttggcg aggctccatg tgaaggcgtc cccgcgcgcg tctttccacg ccttgatcgc
     4501 cagcccctct accatggtca cacagttgtt ctgcctcggt ggcgcctgct gaatgctgac
     4561 gtcgggaagc tcgtacggat accgtccgtc gtcgagcttg tacgagaagt tcttgaagtc
     4621 gtcgatcctg tctatcagga atgactcttt gacgctgttc ggggccgcct cgagctccac
     4681 ctcgatgtca ttcgcgggcg gggagtgcct ctcgctctca gcgtcgaagt ggttctcgat
     4741 gacgaaccgc aggcggaaaa gccgcttctc ggccaccacg accttatact ctccctgggc
     4801 gttcgtggtc gcggccccct ccttctcaac ggtgtcgtaa aggagggcgt agtggctgtc
     4861 cgtcgacctg gagcaaaccc acccctcgcc gccggggagc cccgcggact ggacccgcac
     4921 gtagtcggcg tcacccacgt tgcgcaggat ctcgaatacc ttgtagtcac cctcgttcag
     4981 gctgcccgtc tgcttcgatt tggtgtccgg tttctcgaag gtggacagct tcctcgtgag
     5041 cctgagctgc gtaccgcgca agtccaccct ggcgttggca accggattgc ggctgatctt
     5101 atcgatagct ttaccttcga acgcaagtat ctccgtaggc ggtggcaagg gagcctcgtc
     5161 gcccgcgggc ggcgtggtcg tgtcggtggg ttcttcagta ggaggagtcg cttgctcgtc
     5221 gaggccggcg tggtcgcggg cgtcgcgctc caaccaggcg tcctcgtagc cgtgcaggat
     5281 cacgtcccac cagtacacgc cgaatatggg cagtccaagg tggaagaagg gcccgaacca
     5341 gccgtactgg ttggtgtgcc tcagctcgtg ttcgtagagg gcctcgttct tagggatcgc
     5401 caccttgccg tcgtaggcgg gcgggtagac gtcgccagga ccgctggagt agggttgctc
     5461 gtcccacttc ccgtaaacga aaaagacgtt gccgaaggtg atcccgagca gacgctcgaa
     5521 ggacccgagc cagccccccc tgaacacgag cgcgaaggcg ttgaggtgcc ccgaggcggc
     5581 gatgtcgaag cctggcgtct cccagctcaa gttcccgaag ctgacgagaa agacgagcca
     5641 gcgcagaacc tcgccgacga ggcaggtgat ctccaccaac agcccgaaga tggtgttggg
     5701 cagcgtccag atggcaccta gtatcgagag cagcgtccct ccgaacgacc agtggccgtt
     5761 cgcgtcgatc ctcccgacag ggtcgttgcc gcagaaggcg tagcggttgc gcactctcgg
     5821 ctgcttgagc cagtcggcca ggatatggga tctggcgatc gcctgcgagc cggcggggcg
     5881 gcagggatgg acgagccgct cgtcgtcggg gcgtcccgta tacgtgtccg gtgtcaggaa
     5941 acgaccgagc ccgggatcgt accagcgggc accgaagtag tacagcccca cctcagggta
     6001 ccagaccctt ccgccgaaca tcgggaggtg cttctccgcg gggcccgaag gcactccgaa
     6061 cgggtcgtac tcccaccttg cggcgacgcc accgtcctcg cctgtaacga ggtggcgcgt
     6121 cccccgatgg tcctgatggt ggaagaacac gctgcccgtt tcaacgctgc cgtggatctc
     6181 ggccaggaga cccatgggcg tccagacgta cagccgcagt ggagagcctc tttcgtcggt
     6241 gacggcgacg agctggtcgt ccggtccgta gaggtatctc tcggagcgaa cggcagactt
     6301 catcgcggcc aggcgaccct tgtggtcgta ggtgaagcgg gcgacggatc ttccccggtg
     6361 gcgggcctcc agaagctgcc ccgcgtcgtt gtagcggtac gcccatcgcc cctccggagt
     6421 ggccttccgt acgggccgcc cgaaatcgtc gtaggagatc ctgaaggagt cgccgccggc
     6481 tacctcgacc agacggttgt ctgcgtcgta actatacttg cgggtaccat ccggcccgcc
     6541 ggcctcgacg cggttgtcct gcgcgtcgta gagataacgc caccgccgtt ccgtacgtac
     6601 ctcatccacc ccgatcaaac ggtcctgcgc atcgtagtgg aaacaccggg ctccatctga
     6661 agccagcctg ccctcgaggt cgtatgcgtg catccgctcg aagagaagat ctctatcttt
     6721 gcgtatccgc agatggacag gacggtggtc tacctcgtcg gccccggtct cttccacgac
     6781 accgttgcgg aggtggactc gcgaacacgc cggcacatcg aggtactcga aacgggcgag
     6841 cggctcctcg cccagcgcca cggtgtgcgg cctccccttc gcgtcccatg tgtagcggat
     6901 cggggcatcg ccaccgggaa gccggaactc cgcgagcctg ccagcggcgt cgtactcgag
     6961 tcgcagttct acggcgacgc catcacagct ctggcggatg gccgctatct ggccgtcctg
     7021 gtggtaggtg tgcgttgtcg agacggtcga agtcctcgct tcgacgaccc gacctcgctc
     7081 gtcgtggcgg tagacggagg ctcccgcgtt cccgtgcctg atcctggtga gcctgccttc
     7141 ggcttcgtac tcgaagcgcc gcactacgcc gcgttcggcc acgcttgaca ggcgatcttc
     7201 cgcgtaagcg tagctagtcg aagttccgtc gggatggatc acgcgcgcga ggcggcgggc
     7261 ctcgtcgtac tcgaacgcgg tgctccgccc gttcgcctcg acgatcccgg tgaggtcgcc
     7321 gcggcggtcg taggcgtact cgaagcggcc gcctcccgcc tcctccaccg cgtcgatctg
     7381 acccgagggg gcgaacacct cgcgccgctg cccgtcgtgc agcagccagg cctcgacacg
     7441 gcgcgcggcc tcgtcgccaa ggcgggtacg ctccgaactc gtttctacca ccgcgcctga
     7501 ccgtctcatg ccgccacccc gcctgatgcg cccgatgaag acccgagtgg gatggcggcg
     7561 agcatctcgt cgaaggcgtt acgcaccctg ccgagctcct tatcgaagct caggccgagg
     7621 ttgtcgatca ggcgcttcag gcgttcgagc agctcgacga agaccagttc ctcaagacgc
     7681 tctatgacct ccttcaccct tcccgctgta gttcccaggg tcgtttcgat ctggttgagg
     7741 agcttttgta tctcctcgcg catagccttg agttgttctt cgagggcctc tgcgatgtcg
     7801 tccaggacct ccctgaccgt ggtggacacg gcgtcgaggg tctcgcggta ggtctcatcg
     7861 atctctccct ttatcgtcgc aggatttatg tcctgcagcg ccccggtcag cgggtcgaag
     7921 aagttctcgc gtatggagtc ggcgagcgcg tcagggtcga ggacgcgtac cttcctgcgc
     7981 actgcctcat agatgtcctc cacggcgtcc ttcaggtcga tggggttcag gaacatcaga
     8041 gcttctcgta tgcccttgaa gatgccgtcg aaggcaggtt cgagcgcctc cccgagcgga
     8101 cttacctccc ggaggaaccg ttcgaggacc tcctccaccc tgtcgaactt cacggaggga
     8161 cgcatacccc ttagccccgc catgatctcg tcgtgcgtca ggggggtcgg gctgcgcagg
     8221 aaaggcggca ccacactgtc gaggcttttg cgcaggtcgg tataaacctc cacggcgccc
     8281 gaggcgtcga gggcgttgat cctgcgccgc agcgcgtccg ccagggcgtc gtgggcctcg
     8341 atcaggggct ggatgaggct cgcgtccacg cctggggcga ccagcgagat caccgcgtcg
     8401 aagcgcgccg atacggtggc tacatcactc tgccggcccg gcggagccgc ctctgccttc
     8461 gcctcgaagg cgagcttgag cggcggcagc gcctgcggcg ccagcagcct cctggggtcc
     8521 agctccacca gcctggcctg tccctcgcgg aagacacgga tgacagcgtc ggggtcgagg
     8581 gcttcgagcc cgaacccgag ccccgtacgc acggcgttca tggcgtccgt caggtcgtct
     8641 gccggtatct ccccgaccat cccgataagt tcgtcgaata cggcgtcaag cggctcggcg
     8701 agacggcgca gcgagacctc ggtgttcacg cgggtgctga tctgcttcag cgctgtatcc
     8761 gggtcgccga agatggcctc ggtcacgtcc tcgacgaagg ggcgcatgcc tgtgtagaag
     8821 cctgcgaagg gctccggcag gtcgatggcg tcgaaggcgt ccatgatcgc cgtccggacg
     8881 ttggagagaa gctccctctg catcctgtcg agctcgtcga gcagaggggt cacgtcaata
     8941 aggtcgatca cgttgtcgat ctgctcgtag aggtcgttga ggggagcgac ccactgggcg
     9001 gggtccaggc ggttgaccgt ctccatcatg gtgtcgtagg gcgcctgcag cgggtcgagc
     9061 acctgtccgg gggagaactc ctccagcttc tcaaccaggc tgtcgacctg cttctgaagc
     9121 ggacgcacca gagcctggcc gttcaccttc tcgacggccg cgccggcagt gtccaacgcc
     9181 tcgcccacgg ggccgagcac ctgtccgggg tcgaagccct cgatcgtatc gcgcagttgc
     9241 tcgagcgccg ctgtgatctc ttcgagcaaa cccttgatct cagcctcagc ggccctgtat
     9301 ccctgtttga gacccgagat gacctggtct tcgaggtcga tctcctcgag cagggcgagc
     9361 gccgccttga gggcgagctt ctccacgtcg gagagcgtgt tcgggtctat ggcctcgagc
     9421 ctcgtcttca gctcgtcgat ctgctcgatg acctggtcgc ccacgggctc gaagctcagg
     9481 gtgtcgagct gggagaggag gctgctcagg tcctccatgt acccttcaat agccgtctgg
     9541 agctccgcgg tcagggtatc gagcttgttc acggccgcgg tcagctcgct gatcagggtg
     9601 tcaaggccaa ggctcttgat gttggccagg acgagctcca cagccccttg cacgccctct
     9661 gtcagcccgg agttgatgtt ctctgtcacg aactcccgcg cctcgtccag cgcggccgag
     9721 atgctctctt tgatctgggt gatgccgaga gcctcgatct cgtccccaac ccgctccagc
     9781 atggcctcta cggtcagctg gatctccgcc gttgggacgc tgtcgatagc gttttcgatg
     9841 tccccgagga aagccctgat ggtctggcgc acctggccga gcggggaggc ggcaaaagta
     9901 tcgtggatga ccgcgcttaa tgcttcgatg cgctgcgtga ggtcctggac cccgaaggtc
     9961 atgtagaacc gcgccagtat ctcttcgagc acctcggcca tctcgtccgt gacggaatcc
    10021 acggtgaaag gcggatcgaa ggttacttct tgcaggagat cccggtacgt cgagaagacg
    10081 tcgtcccacg cgtggttctc caccagggtc tgaacctgcc cgtacaccgg cgtgagcgcg
    10141 gccaccatcg ccctgatggc ggactcgagc ccgccgagca gcgtctccag ttcgtcgtac
    10201 gccgcggcat ccgcggggtc gaactcctcc accgcggcca gaagatctgt cagggagccg
    10261 gtgagatcct cgcgggccgg gttcaacgcc tcggcggcca gcggcgcgag gacggcgtat
    10321 gtttcgtcga ggtgttgcag gggaccactc aagaggtcgg gcctgacgcc gagcaggtgc
    10381 tgcgtgagga aaggcaggag gtcatcgctg tgggactcga agtcggtacg gaactcctcg
    10441 atgctcgcga agacccccct gatcccttcc agcgtatcag cgtcgatcag gttccccccg
    10501 agctggccga tacgctggtc gaagaggccg agcgcctcct cgatcacggc gagcgcgacc
    10561 tctttgaggt cacccccagc ggccacctcc tttacgagag cgttgtcctg aagtaggtct
    10621 cgggccgcgt cgtatgctcc ctcgatcgcc tcgacgtact cgagcgtacc cggtggcagt
    10681 gcgttcttta tgacgtcgat ggcggacgac aggtccgtgg agagtcctgt ggtgtcgatg
    10741 tcgagcttcg cctccagatc gaccagcaca ctgcccacgg gcgcgaccgg ggaggaggaa
    10801 ttgcccggct cgaacgaggc gagcgtgccg aggaatcctt cgagttgacc ctccgccgca
    10861 cttcgcctaa ccagcagagg ctggatatcc ggcagctgct gtagtatccc gtccgccacg
    10921 gtcagaacag cttcctgatc tcggcgagca ctgccgcccc ttcgccggca gcctgcacga
    10981 acccgctctc tatcccgggc agggactcga cggggttccc gaacgccggc gtgctcagga
    11041 ggtcagccag ctgggagacc tgctcgagcg cgttctgtac gtcgtccacg aacgaggccg
    11101 cgtcttcgag aaggctcgta tcgagcgccg cgaaggggtc agcgatcacc ggttccggag
    11161 gctccacgta ctcgaccacg tcgcagacga agtcgaactg gtccaggtag ccggcgcgct
    11221 gggatatgtc gagccgcgag atgagcactt gcgtgaagta gccttcgcca accgattctg
    11281 tgaagaag
//
LOCUS       DATAIR010000110         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_6858, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000110 DATAIR010000000
VERSION     DATAIR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6858"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VHM69_02795"
     CDS             complement(<1..246)
                     /locus_tag="VHM69_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX2739350.1"
                     /translation="MQIQSNPYRTHGAGELRKGNVGETVRLAGWVHRRRDHGGLIFID
                     LRDRWGITQVTFHPERAETFSSAEKLRPEWSICIEGEV"
     gene            494..1447
                     /locus_tag="VHM69_02800"
     CDS             494..1447
                     /locus_tag="VHM69_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739351.1"
                     /translation="MALLIACVVLLAGCGGGDESEAPTPPAPSSPSPDAVSVPADNAA
                     VGVGQAESTGDLPFTLNTQQPVPPNFEEAYGRRARIVVQFYKLDADSLSYPQGLSVDR
                     QVNRSMEQLSAQYPTIEFFSYDISDPGPVSGERSLKQGEYGTLAAQLGVGITPFVAML
                     APSGDEYVITNLFQGYVPRPVLSQALFDLAAVEVEDNTSDVNVILDQVEMTESGGGIE
                     FFSVRNRSADAINLQGFTLRVLDPETAQVTPGAPGVTINSDIRVEPGQSVSIGRVPDV
                     VDDDGERVAGTFEGGTSLDLAPGDQIALLDPGGAVASTITV"
     gene            1509..2669
                     /locus_tag="VHM69_02805"
     CDS             1509..2669
                     /locus_tag="VHM69_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2739352.1"
                     /translation="MAEPVYLDNAATTPLDPRVLESMLPHLGGRRGNPSSLHALGYAA
                     RDALEDARESVASLIGASPEEILFTSGGTESDDLAVLGLARPATPEKRHIVVSRVEHA
                     AVREAAKRLHSEGFEVSWIGVDAKGMVDPDEFAAALRPDTALAAVVWANNEVGTIEPV
                     QELAQLCAGSEIPFHADAVQAAGRVPLDVSEVPVSTLALSGHKLYGPQGIGALYVRDG
                     VILEPLVYGGGQEKGLRSGTQNVAGIVGLGTAARLAREELDDRVRHESDLRDRILAGI
                     EVIPDVRVNGHREKRLSNNVHLTVDGVEAESLVLLLDSLGYAIGSGSACSSGGHKASQ
                     VLLAMGVPEREAFSVVRITVGKENTAEDVEGFLAAFSTAVAQLRELSPLYAG"
     gene            2682..>2888
                     /locus_tag="VHM69_02810"
     CDS             2682..>2888
                     /locus_tag="VHM69_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX2739353.1"
                     /translation="MSDRYTKQVMDHLVRPRNAGEIPDPSGRGESGDASCGDVACFTV
                     GISENVLEEVRYKVYGCAACIAAGS"
BASE COUNT          515 a          902 c          959 g          512 t
ORIGIN      
        1 cacctcgccc tctatgcaga tagaccactc cggtctaagt ttctcggctg aggagaacgt
       61 ctcagcacgt tcagggtgga atgtgacctg cgtgattccc cagcggtcgc gcaggtcaat
      121 aaagatcaga cctccgtggt ccctgcgcct gtgtacccag ccggcgagcc tgaccgtttc
      181 acccacgtta cccttgcgaa gctcgcccgc gccgtgcgtt cggtacggat tgctctggat
      241 ttgcaacttg gatctccttg tggtagccgg cgccccgctc ctgagcgggt ccacgaatgg
      301 tatccgaaaa ccgccgcgac cgaagcgtcg tttgtatcgg gcgggcgcgt tgcggtgttt
      361 tcacgaccgt gctaacttag cgcaaatcca gtcggaggcc cgtgcggttg cgccgggtct
      421 ccagagagct gcaggggaga ggtctatccg aagaaacggc aagggaggat cacccgcggg
      481 atcagggctg ctcgtggcgc tcctcattgc ttgtgtggtt ctccttgccg gatgcggcgg
      541 gggagatgag agcgaggccc cgactccgcc ggcgccgagt tccccttcgc cggacgccgt
      601 aagcgtcccg gcagacaacg cggctgtcgg ggtagggcag gctgagagca cgggagacct
      661 gcctttcacg ctcaacaccc agcaaccagt tccgcccaac ttcgaggagg cgtacgggcg
      721 cagggccagg atcgtggtcc agttctacaa gctggacgca gattcgctct cgtatccgca
      781 gggactctcc gtcgaccggc aggtaaacag gtcgatggag caactcagtg cgcagtatcc
      841 gactatcgag ttcttcagct acgacatctc cgaccctgga ccggttagcg gcgaacggag
      901 tctgaagcaa ggcgagtacg gtacgctcgc cgcgcagctt ggagtcggga tcacgccgtt
      961 cgtcgcgatg ctcgcgccga gtggggacga gtacgtgata accaacctgt ttcagggata
     1021 cgttccgagg cccgtattga gccaggcgct cttcgacctc gcggcggtag aggtcgaaga
     1081 caataccagc gacgtgaacg tgatcctcga ccaggtcgag atgaccgaaa gcgggggcgg
     1141 catcgagttc ttcagcgtac gcaaccggag tgcggatgcc attaatttgc agggcttcac
     1201 cctgcgggtc ctggatcccg agacggccca ggtcacccca ggcgcgcccg gcgtgacgat
     1261 caacagcgat atcagggtcg aacccggtca gagcgtctcg ataggccgcg tgcctgatgt
     1321 ggtcgacgac gacggcgagc gcgttgccgg cacgttcgaa ggaggcacgt cgctggatct
     1381 cgccccaggc gaccagatcg cactcctcga ccctggcggc gcggtcgcct cgacgatcac
     1441 cgtctgaacg ggagatatag cgctctacgg ctgacatttc ggccctttag gataaactag
     1501 gcgcgatcat ggccgaacct gtatacctgg ataacgccgc caccaccccg ctcgacccga
     1561 gggttctgga gtcgatgctt cctcacctcg gcgggcgacg ggggaatccg tcttcgcttc
     1621 acgccctcgg gtacgcagcg cgcgacgccc tggaggacgc gcgagagtcc gtggcctccc
     1681 ttatcggggc ctcgcccgag gagatactgt tcaccagcgg cggtacggag tcagacgacc
     1741 ttgcagtcct tggtctggcg cgacctgcca ccccagagaa gcggcacatc gtcgtctcgc
     1801 gcgtggagca cgcggcggtg cgggaggcgg cgaagcggct gcactccgaa ggattcgagg
     1861 tctcctggat cggggttgat gccaagggta tggtggaccc agacgagttc gccgccgcgc
     1921 tgcgccccga tacggccctc gccgccgtgg tgtgggcgaa caacgaggtc ggtaccatcg
     1981 agcccgtgca ggagctcgca cagctctgcg ccgggagcga gatccctttc cacgccgacg
     2041 ccgtccaggc tgcgggtagg gtcccgctgg acgtctccga ggtgccggtc tcgacgctgg
     2101 cgctctcggg acacaaactc tacggcccgc agggcatcgg cgcgctctac gtgcgtgatg
     2161 gcgtcatcct cgaacccctc gtttacggcg ggggacagga gaaggggctg aggagcggca
     2221 cccagaacgt cgcaggtatc gtcggattgg gcacggcggc gaggctggcc cgcgaggagc
     2281 tggacgaccg cgtcaggcac gaaagtgacc tgcgcgaccg catcctggcc ggcatagagg
     2341 tcattcctga tgtgcgggta aacgggcatc gcgagaagcg gctgtcgaac aacgtccacc
     2401 tgaccgtaga cggcgtcgag gccgagagcc tggttctctt gctggactct ctcggctacg
     2461 caatcggaag tggctcggcc tgctcgtcgg gcgggcacaa ggcgtcgcag gtactgctgg
     2521 cgatgggcgt gcccgagcgg gaagctttct ccgtcgtcag gatcacggtc ggcaaggaga
     2581 acacagccga agatgtcgaa ggtttcctcg cggccttctc gaccgcggtc gcccaacttc
     2641 gagagctctc cccgctctac gcgggctgaa ggctggatat tttgtcggat cgctacacaa
     2701 aacaggtcat ggaccatctc gtcaggccgc gcaatgcggg agagatcccc gacccttctg
     2761 gccggggaga gtcgggcgac gcatcctgcg gcgacgtcgc ctgctttacg gtcggcatct
     2821 cggagaacgt tctcgaagag gtccgctata aggtctacgg ctgcgcggca tgtatagccg
     2881 ccggctcg
//
LOCUS       DATAIR010000111         6103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_421981, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000111 DATAIR010000000
VERSION     DATAIR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421981"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHM69_02815"
     CDS             <1..328
                     /locus_tag="VHM69_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2739354.1"
                     /translation="ERTVLKLHPRIAPTKAAVFPLSKKEPVSTIARELYDDLKGDYRL
                     FYDDSGSIGRRYRRQDEAGTPFCVTVDFDTIEDKEVTIRDRDSLEQERVPIAAVRDRL
                     KALISG"
     gene            complement(329..739)
                     /locus_tag="VHM69_02820"
     CDS             complement(329..739)
                     /locus_tag="VHM69_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2739355.1"
                     /translation="MGEALITLDTSGIFALLNRRDRDHERVKTALFADKGPYLVPAGI
                     LAEIAYMVEERLDTRALESFILDLESGGLSLECGEEDLPRIRELVGRYEDLPLGFADA
                     SVISCAERNGGRVLTLDLRHFGVVAAEGEISLLP"
     gene            complement(724..948)
                     /locus_tag="VHM69_02825"
     CDS             complement(724..948)
                     /locus_tag="VHM69_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX2739356.1"
                     /translation="MEKTTVYLPAHLQRALKEAARSEKKAQAEVLRRALEEYLERRER
                     PRLRSVGIGEDEELSGAESEDWLRGEWGKR"
     gene            complement(1184..2812)
                     /locus_tag="VHM69_02830"
     CDS             complement(1184..2812)
                     /locus_tag="VHM69_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2739357.1"
                     /translation="MASNAAADSSTGDLFQRKATGLLRGWSVWDAFIYATFSINLITL
                     GLFILSYSPFIPDGALLPAIILSGLYLIFQAVTYASLIAVMPRAGGDYVWMSRILGGG
                     IGFVLAVTGWWFILWHWVPIYADILVKEVFVPISAILGFDGAVAFFGQPGGVFTASVI
                     VAVLASVLISLGMRSYARIQKLCFYGGLVGLAIMLVILLVSSRADFISAFNAEASQTL
                     GASGNAYQETIRAGDQAGGLPAGAGVYPTLLLIPFLAFFNLFSNWGATLYGEVRGASD
                     FRKNIYAMGGALVVTSVTAVIMLMLFAKTFGWDFYYGSNAAYWGLVYGTIDEAPVSAW
                     PYPALLVSFLLGNPMLQVILIALMSLWFFGWVGTVFMSSTRVVFATAFDRVLPEAVAK
                     TSRNGVPWVALLLMLIPSIPISYFYAYGENFATWTLNATLVIALTFFGSTVAAAILPW
                     RKPEIYNASPIARYNVLGIPLVTVCASAFGLFLAFCIYQWVTNDLYGINNTSSFIYLG
                     TMYVAALVIYVVSMFVRRAQGMDLKMVYDEIPED"
     gene            complement(2825..3799)
                     /locus_tag="VHM69_02835"
     CDS             complement(2825..3799)
                     /locus_tag="VHM69_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739358.1"
                     /translation="MISRLFSRGPETRIFFATDIHGSEVCWRKFLNSGKHYEAKVMIL
                     GGDMTGKALVPVVEEGSGRWHATLLENRREFESEDELEEFEDSVRNRGYYPFRTDPDK
                     MSELESDEELRDKLFHEEMLGTVERWMRMADEKLEGTGIECFVSPGNDDQFEVDEIIS
                     SAKLVRLAEGEVVEFGDFQMVSTGWSNRTPWDTYREEDEEDLAERLRKMTSQVTAPPE
                     KSIYNFHCPPYRSGLDDAPEIDEQMRPKHGGRSIVPVGSKAVREAIEEGQPGLALHGH
                     IHEARGNTRIGNTLCINPGSSYEQGQLLGAVVDLDGGKKVKRFVLTSG"
     gene            complement(3796..4569)
                     /locus_tag="VHM69_02840"
     CDS             complement(3796..4569)
                     /locus_tag="VHM69_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739359.1"
                     /translation="MDNQSTDGIRGDMVEEAVRVAEEAERKDVALRLLGGVAIKVRAR
                     DGLNPAFEREYADLDWITPKGKSAQAQKFFESLGYAPQKRFNAMYGRERLLYFDEEND
                     RQVDVLVGTFRMCHEIPFGDRMSLEPLTVPLAELLLTKLQIIELNEKDVRDALAILHD
                     HPVEEQDGDSVNAAHIAKLCGSDWGLWRTFTANLEALEGHLERYDLPAENKERIMDRI
                     RALLGRIEEEPKSFGWKMRAKIGDRKRWYDLPEEVEGGP"
     gene            complement(4575..5561)
                     /locus_tag="VHM69_02845"
     CDS             complement(4575..5561)
                     /locus_tag="VHM69_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2739360.1"
                     /translation="MQATEGEAAKGRDRPGGGYRPNGTNDPWKKTGMLAPLLTFLKTS
                     LLLLVAGVFAAGCGFGGAGELTLGYLGWDENVANSNLTKVLLEDELGYHNVELKLADD
                     VRPLYDDLIEGNTDAFLDAWMPNHQQFVDRGGIEVSKEPWYVGRTRYGIAVPKYMHDV
                     RTIPDLNSSGTEMLTGIEPGAVLMTKIESDVVPQYDLRFALVEASTPAMLAELEQAYT
                     MKEPFVFLAWSPHWMNQEYKFRYLSDPKNAMGGVDDPQRIHSVTREGLAGDEPAAYAL
                     INAMRLNEEQVGSIELAINRADDPEKGVRRWLGENRDLVRPWIEAAKNAQEG"
     gene            5826..5898
                     /locus_tag="VHM69_02850"
     tRNA            5826..5898
                     /locus_tag="VHM69_02850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5859..5861,aa:Arg,seq:cct)
BASE COUNT         1173 a         1935 c         1802 g         1193 t
ORIGIN      
        1 ggaaagaacg gtgctcaaac tacatccccg catagccccg acgaaggccg ccgtcttccc
       61 gctctccaag aaagagcctg tctctaccat agcccgcgag ctctacgacg acctcaaagg
      121 cgactaccgc ctcttctacg atgactcggg ctcgatcggg cgccgctacc gccgccagga
      181 cgaggcgggc acgcccttct gcgttacggt ggacttcgac accatcgagg acaaagaagt
      241 gaccatccgc gaccgcgact ccctggagca ggagcgtgta cccattgctg cggtgcggga
      301 ccgtctgaag gctctgatct cgggttaatt atggcagcag gctgatctct ccctccgcag
      361 ctacgacgcc gaaatgcctg aggtccaggg tcaagactcg tccaccattg cgctcggcac
      421 aggagatcac actggcatcc gcaaacccga gcggcagatc ctcgtagcgc ccaacgagtt
      481 cgcggatgcg aggcaggtct tcctcgccac attccaaact caagccgcca gactcgaggt
      541 ccaggatgaa actttcgagc gccctggtat cgagacgctc ttcgaccatg taggcgatct
      601 cggcgagtat cccggccgga accaggtaag gtcccttatc cgcgaaaagc gcagtcttta
      661 cccgctcgtg atctctatct ctacggttga ggagggcgaa gattcccgag gtgtcgagcg
      721 tgatcagcgc ttcccccact caccacgtaa ccagtcctcg gactcagcgc ccgagagttc
      781 ctcgtcttcg ccgatgccca cggagcgtag ccgtggacgc tccctgcgtt ccaggtactc
      841 ctccaatgcc ctccggagca cctccgcctg tgccttcttc tctgagcgag ccgcctcctt
      901 gagagcccgc tgcaggtgag caggtaaata tacggtcgtc ttctccatag tgatcagttt
      961 atcaccatat accatacgtc atatagtcag gagacaggct ggcaacttga tctaccagct
     1021 ctcgtcagcg ggcaaagtct gaggaactcc cggtcgaccg gacgggcagc gagtcataac
     1081 cccagcgtct gcggggctcc gttgacctgg caatcgatcg tgccaagacc atcgaagatc
     1141 cgcgggcgga acggtatgtt tatcctgttc gtaagttggg ctgctagtcc tccggtatct
     1201 cgtcgtagac catcttgagg tccatgccct gcgcgcggcg gacgaacatg gagaccacgt
     1261 agatcaccag tgccgccacg tacatcgtgc cgaggtagat gaaggaagac gtgttgttga
     1321 tgccgtagag gtcgttggta acccactggt agatacagaa ggcaaggaag aggccgaagg
     1381 cgctggcgca gaccgtgacc aggggaatac ccaagacgtt gtagcgcgct atgggggagg
     1441 cgttgtagat ctcaggcttg cgccagggca gtatcgcggc cgccaccgtc gagccgaaga
     1501 acgtcagcgc gattacgagc gtcgcgttga gagtccacgt ggcgaagttc tccccgtacg
     1561 cgtagaagta gcttatgggg atggacggga ttagcatgag caacaaagcc acccagggga
     1621 cgccgttgcg gctggtcttg gcgaccgcct cagggagaac ccggtcgaag gccgtggcga
     1681 acacgacgcg cgtcgaggac ataaagaccg tcccgaccca cccgaagaac caaagcgaca
     1741 tcagggctat caggatcacc tgcaacatcg ggtttccgag gaggaaggag acgagcaggg
     1801 ctggataggg ccacgctgag acaggagctt cgtcgatggt gccgtagacc agtccccagt
     1861 acgcggcgtt ggaaccgtag tagaagtccc agccgaaagt cttggcgaag agcatgagca
     1921 tgatgacagc cgtcacggag gtgacgacga gcgccccgcc catcgcgtag atgtttttac
     1981 ggaaatccga agcgccgcgg acctcgccgt agagcgtggc gccccagttg gagaacaggt
     2041 tgaagaaggc gaggaagggg atcaagagca gcgtcgggta gacgccagcg cccgcgggga
     2101 ggccgccggc ctgatctcct gcccttatgg tctcctggta ggcgttgcca ctggcgccga
     2161 gcgtctggct agcctcggcg ttaaaggcgg agataaagtc cgcgcgcgag ctcacgagca
     2221 ggatgaccag catgatcgcg agtcccacga ggccgccgta gaagcagagc ttctggatgc
     2281 gggcgtagga acgcatcccg agtgagatca ggaccgaggc caggaccgcc acgatcaccg
     2341 aggcggtaaa gacgccgcca ggctgtccga agaaggccac ggccccgtcg aagccgagga
     2401 tcgcggagat cggaacgaag acctccttga ccaggatgtc agcgtagatc gggacccagt
     2461 gccagaggat gaaccaccac cccgtgaccg cgaggacgaa cccgattccg ccgccgagga
     2521 tacggctcat ccagacgtag tctccaccgg cgcggggcat cacggctata agcgaagcgt
     2581 aggtgacggc ctggaagatc aggtagagcc ctgagaggat gatcgcgggc aagagcgcgc
     2641 cgtcgggaat gaagggcgag taagagagga taaagaggcc gagtgtgatc aggtttatgg
     2701 agaacgtcgc gtagatgaac gcatcccaca cggaccagcc gcgcaagagt cccgttgcct
     2761 tcctctgaaa gagatccccc gtactagaat ccgccgctgc gttgctggcc atcgatctcc
     2821 tcctttatcc gctggtcagt acgaaccgct ttactttctt gccgccgtcg aggtcgacga
     2881 ccgccccgag cagctgcccc tgctcgtacg agcttcccgg gttgatgcag agtgtgttcc
     2941 cgatgcgcgt gttgccccgc gcctcgtgga tgtgcccgtg cagcgccagg ccaggctgtc
     3001 cctcctctat cgcctccctc accgccttcg agccgacggg tacgatcgag cgcccgccgt
     3061 gtttgggccg catctgctcg tctatttcgg gcgcgtcgtc cagccccgag cgatacggcg
     3121 ggcagtggaa gttgtagatg gacttctcgg gaggggctgt tacctgcgag gtcatcttcc
     3181 gcaggcgctc ggcgaggtcc tcctcgtcct cctccctata agtatcccag ggcgtgcggt
     3241 tggaccatcc tgtggagacc atctggaagt cgccgaactc gacaacctcg ccttcggcaa
     3301 gccgcacgag cttggctgag gagatgatct catcgacttc gaactggtcg tcgtttccgg
     3361 ggctgacgaa gcattcgatc cccgtcccct ccagcttctc gtcggccatg cgcatccagc
     3421 gctcgacggt ccccagcatc tcttcgtgga agagcttgtc gcgcaactcc tcgtccgact
     3481 cgagctcgga catcttgtcg ggatcggtgc ggaacgggta gtagccgcga ttcctgaccg
     3541 agtcctcgaa ctcttctagc tcgtcctcgc tctcgaactc ccgccggttc tccaggagcg
     3601 tcgcatgcca gcggccagat ccttcctcga ctacggggac gagcgctttg cccgtcatgt
     3661 cgccgccaag gatcatgacc ttcgcctcgt agtgcttgcc cgagttcagg aacttgcgcc
     3721 agcagacctc cgagccgtgg atgtccgtgg cgaagaagat ccgggtttcg ggtccccggg
     3781 aaaagagtct agagatcatg gtcccccctc aacctcctca ggcaggtcgt accagcgttt
     3841 gcgatcacct atcttcgcgc gcatcttcca gccgaaagac ttcggctcct cctctatgcg
     3901 cccgagcaat gctctgatgc gatccataat gcgctccttg ttctccgcgg gcaggtcgta
     3961 gcgctccagg tgaccttcca gggcttccag gttggccgtg aaggtgcgcc agaggcccca
     4021 gtcggagccg cacagtttgg cgatatgggc ggcgtttaca gagtccccgt cctgttcttc
     4081 cacgggatgg tcgtgcagga tggctaaggc gtccctgacg tccttctcgt tcaactcgat
     4141 gatctgtagt ttcgtcaaca ggagctccgc tagtgggacg gtcagcggct cgagggacat
     4201 acggtcgccg aatgggatct catggcacat cctgaaggtt ccgacgagta cgtccacctg
     4261 cctgtcgttc tcctcgtcga agtaaagcag ccgctccctg ccgtacatcg cgttgaagcg
     4321 cttctggggt gcatatccca gagactcgaa gaacttctgc gcctgggcgg atttgccctt
     4381 cggtgtgatc caatcgaggt cggcgtactc gcgctcgaag gcagggttca ggccgtcgcg
     4441 cgcgcgcacc ttgatagcca cccctcccaa taggcgcagc gccacgtcct tgcgttccgc
     4501 ctcctctgcg acgcgcacgg cctcctcgac catgtcgccc ctgataccgt ccgtgctctg
     4561 gttgtccata tctactaacc ctcctgcgcg ttttttgcgg cctcgatcca gggccgcacc
     4621 aggtccctgt tctccccgag ccacctgcgg accccctttt cagggtcatc agccctgttt
     4681 atggcgagct cgatgcttcc tacttgttct tcgttgagcc tcatggcgtt gatgagggcg
     4741 taggccgcgg gttcgtcccc ggcgagtcct tcccgcgtga ccgagtgaat cctctgaggg
     4801 tcgtccacgc cgcccatcgc gttcttcggg tccgaaaggt agcggaactt gtactcctgg
     4861 ttcatccagt gcggcgacca cgcgaggaac acgaaaggct ccttcatcgt gtatgcctgc
     4921 tcgagctcgg cgagcatcgc cggggtggag gcctcgacca gggcgaagcg cagatcatac
     4981 tgcggcacga cgtcggactc gatcttcgtc ataagcaccg cgccgggctc tatgccggtt
     5041 agcatttcgg tccctgatga gttgaggtcg ggaatggtcc gcacgtcatg catgtacttg
     5101 gggacggcga tcccgtacct cgtccggccc acgtaccaag gttcctttga cacctcgatg
     5161 ccgcccctgt ccacgaactg ctgatggttc ggcatccagg cgtccagaaa ggcatcagtg
     5221 ttgccctcga tcaggtcgtc atacaggggt ctgacgtcgt cggcgagctt gagctcgacg
     5281 ttgtggtagc cgagttcgtc ttccagcagg actttggtca gattggagtt ggccacgttc
     5341 tcgtcccagc ccaggtaccc caacgtgagc tccccggcgc caccgaaccc gcagcccgcg
     5401 gcgaagaccc cggccacaag caagagcagc gaggtcttga gaaatgtcag gagtggcgca
     5461 agcatccccg ttttcttcca cgggtcgtta gtcccgttcg ggcgatagcc tcctccgggc
     5521 ctgtccctac ccttcgcagc ctctccttca gtagcctgca actaaggatc gcacgtcccg
     5581 ccgcctgcac ttctcccgcg gcgataacaa aatgcatgca acccttgcac cctacccacc
     5641 ctacctgggc ccgagtaccg ctgaatcata cgtcgttccc aacccccatt caactgaatg
     5701 cccgcccgat aaataacaaa cgtatctgaa gttcgagcct gttcctgatt gtgaatggtg
     5761 cgcaaggccc ctcgccacga cacggtggta agcatggtct tcccaggtta aactgttggc
     5821 gctttgcgcc tgtagctcag gggatagagc agcggtttcc taaaccgcgg gtcggaggtt
     5881 caaatccttc caggcgcgct tctcaaccgg cttatctaaa ctgatttcgt ccgccggcac
     5941 tgagccgaag gtcagacccc accagtcatt tcaccagcac agacatctca ggacgacccc
     6001 gagaagcggg cgccgaagcg gctcatggcc tccccggttg ctctgtcgga ggggtggaca
     6061 taactgttcg cgcaggtatg cggccacctt ctcctcggtg atc
//
LOCUS       DATAIR010000112         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_421990, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000112 DATAIR010000000
VERSION     DATAIR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421990"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..205
                     /locus_tag="VHM69_02855"
     CDS             2..205
                     /locus_tag="VHM69_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2739361.1"
                     /translation="MAQGTVKGFSDEKGYGFISPDDGGEDLFVHHTGIAGEGFKTLDE
                     GAKVTYEATQGRKGMQAENVTPA"
     gene            321..1103
                     /locus_tag="VHM69_02860"
     CDS             321..1103
                     /locus_tag="VHM69_02860"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX2739362.1"
                     /translation="MQYVIAVLVGLALAEVATLITTIYLHRVLSHKAIRLHPSLTMFM
                     RFGTWMLTSISPREWVAVHRKHHNFSDVEGDPHSPHIEGYWRIMIGNVYYYRREANND
                     NTLEKYAADLPFDRLDRLFLRRGTLGFSLTGVILVLLMGLGPGLVALTTLAVVYLLLN
                     AAINGAGHTFGYKSFENDATNLRLLALVTFGEGLHNNHHARPASAKLSAFRGEFDPAW
                     PVIKLLVALRLAEVLSKVDEGWEDHRRRIPTTIVTPLGVAKD"
     gene            complement(1313..2275)
                     /gene="argF"
                     /locus_tag="VHM69_02865"
     CDS             complement(1313..2275)
                     /gene="argF"
                     /locus_tag="VHM69_02865"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2739363.1"
                     /translation="MSVVIPDLQSSPPPPSPLSGRDCLTLAEFAPDEVSLILDEAIRI
                     KTLQKSRIPYRPLRGRTLAMVFQKPSNRTRVSFEVGMYQLGGHALHLSPEEIQIGKRE
                     TPSDTGRVLARYIDAIMVRTFDHGDLEELAGAADVPVINGLSDTHHPCQALADLMTVR
                     EELDTVEGTKIAYVGDGNNVAHSLALACALTGADLTIAHPEGHGPDEVIVELAGSLGA
                     APRLTEDPREAVHGARVVYTDVWASMGQETEAEERKKKFAPYQVDDELMDLAAEDAIF
                     LHCLPAHRGQEVTAEVIDGPRSRVFDQAENRLHAQKALLYLLMS"
     gene            complement(2272..>3369)
                     /locus_tag="VHM69_02870"
     CDS             complement(2272..>3369)
                     /locus_tag="VHM69_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HEX2739364.1"
                     /translation="IGGIATNSLGHGHPALVGAIQEQAGKLIHCSNLYEIPLQAEVAG
                     MLTGATDFDRVFFCNSGAESAEAAIKLSRKYAHDTFGPNKHEVLTFSKSFHGRTYGGL
                     TATAQPALQEGFGPMLPGMVHAPFGDLEAAKAMVGPQTAAVLVEPIQGEGGVNVAPEG
                     FLEGLREMCDKYGALLIFDEVQTGAGRTGHLYAYQGTDVVPDVLTSAKGLGGGFPVGA
                     MMVKEEYAALGPGNHGSTFGGNPLAMAAVKAVLGVVNDPAFLEEVRFKGTVLKNALEK
                     LAEQVPGAEVRGEGLLLGLDLPDADLAREVFEHCLDEGVLVNLVGGTTIRMAPPLTVS
                     RTEVRAALDTFRGGVYESMSAMESVPEAAVA"
BASE COUNT          612 a         1055 c         1069 g          633 t
ORIGIN      
        1 tatggcccaa ggaacagtga aggggttcag cgacgagaag ggctacggct tcatctcccc
       61 ggacgatgga ggtgaggacc tcttcgtgca ccacacaggg atagcaggtg aaggcttcaa
      121 gaccctcgac gagggcgcca aagtgactta cgaggcgacc caggggcgta agggcatgca
      181 ggcggagaac gttactccgg cttagaaccc gagaagcaaa agtaagcgca aaggccgggg
      241 tataaacccc ggcttttgtc gtgtaagcag gattatcgga acgtgctggg cgaggcctgg
      301 acccgttcga gggaggtctc gtgcaatacg taatagccgt gctcgtgggt ttggcgctcg
      361 ctgaggttgc gaccctgatc actacgatct acctgcacag ggtgctgtcg cacaaggcga
      421 tccggctcca tccctccctg acgatgttta tgcgctttgg gacgtggatg ctcacgagca
      481 tctcgccgcg cgagtgggtc gcggttcacc gcaagcacca caacttcagc gatgtcgagg
      541 gagatccgca cagcccgcac atcgaaggct actggcggat catgatcggc aacgtctact
      601 actatcgccg cgaggcgaat aacgacaaca ctctggagaa atacgcagcc gacctcccct
      661 tcgaccgcct ggacaggctc ttcctgcggc gcggaacgct aggcttctct ctgaccggtg
      721 tgatcctggt cctcctgatg ggcctcggcc ccggactcgt agcactgacg acgctcgcgg
      781 tcgtctactt gctcctgaac gccgccataa acggcgcagg ccataccttc ggctacaaga
      841 gcttcgagaa cgacgcgacg aacctcaggc tgctggcgct cgtgaccttc ggggagggcc
      901 tgcacaacaa ccaccacgcc cgtcccgcct cggccaagct ctccgccttc cgcggcgagt
      961 tcgatcccgc gtggcccgtg atcaagctcc tcgtcgccct cagattggca gaggtcctca
     1021 gcaaggtgga tgagggttgg gaggaccacc gccgccgcat ccccactacc atcgtgaccc
     1081 cgctcggcgt cgcaaaggac tagagcggtt tgcggaaaga gtgaacctga ggtgagtaaa
     1141 ggtttcaggc tacaggcatc agcaaaaaga cctgatgcct gtagccttct ccgaaggagt
     1201 agctcatcct tttcgagaac cgccatagag gatgggtgat cattgagctc aagccccgga
     1261 ggaaggtctg ttcctatatg ggtcccgccg cctctctcac tatggcctgg gatcaactca
     1321 tcagcagata gagaagcgcc ttctgggcgt gcagacggtt ctcggcctgg tcgaagacgc
     1381 ggctcctcgg accgtctatg acctcagccg tgacctcctg gcccctgtgc gccggcagac
     1441 agtgaaggaa gatggcgtct tctgcggcga ggtccattag ctcatcatcg acctggtagg
     1501 gagcgaactt ctttttgcgt tcctcggctt cggtctcctg tcccatgcta gcccaaacgt
     1561 cggtgtagac gacgcgcgcg ccatggacgg cctcgcgggg gtcttctgtc agacgcgggg
     1621 ctgcgccgag ggatcccgcc aactcgacta taacttcgtc gggaccgtgg ccttcagggt
     1681 gggcgatggt gagatcagcg cctgtgaggg cgcaggctaa ggccagggag tgggccacgt
     1741 tgttgccgtc tccgacgtag gctatcttcg tgccttccac cgtatcgagc tcctcgcgga
     1801 cggtcatgag gtcggcgagg gcctggcatg ggtggtgggt gtcggagagg ccgtttatga
     1861 cggggacgtc ggcggcaccg gccagctctt cgaggtcccc gtggtcgaag gtgcggacca
     1921 tgatggcgtc gatgtagcgg gcgaggacgc ggcccgtgtc cgaaggagtc tcgcgcttgc
     1981 cgatctggat ctcctccggg ctcaaatgga gcgcgtggcc gccgagctgg tacatcccga
     2041 cctcgaagga gacgcgggtc ctgttggacg gcttctggaa gaccatcgct agcgtgcgcc
     2101 cgcgcaacgg cctgtacggt atgcggctct tctgcaacgt ctttatgcgg atggcttcgt
     2161 ccaggatcag ggagacttca tcaggggcga actcggcgag cgtgaggcag tcgcggcccg
     2221 agagggggga tgggggaggc ggggaactct ggaggtcggg gatcaccacg ctcatgctac
     2281 cgccgcctcg ggcacggact ccattgcgct catcgactcg taaacgccgc ctcgaaaggt
     2341 atcgagcgca gctcgtacct ccgtcctgct gacggtgagc ggcggcgcca tacgtatggt
     2401 cgttcctccc accagattta cgagaacgcc ttcatcgaga cagtgctcga agacctcccg
     2461 cgctagatcc gcgtcgggca ggtcgagccc gagcaagagc ccctcacccc gcacctcggc
     2521 gccaggcacc tgctccgcaa gcttctcgag cgcgttcttc agaacggtcc ccttgaaccg
     2581 cacttcttcg aggaaggccg ggtcgtttac cacgccaagc accgccttca ccgcggccat
     2641 cgccaggggg ttccccccga aggtggagcc gtggttgccc ggtccgagcg ctgcgtactc
     2701 ttccttgacc atcatcgccc cgactgggaa gccgccgccg aggcccttgg cgctcgtgag
     2761 cacgtcaggt acgacatccg tgccctggta agcgtataga tgacccgtgc gtcccgcgcc
     2821 cgtctggacc tcgtcgaata tgaggagcgc accgtatttg tcgcacatct cccgcagccc
     2881 ttccaggaag ccttcaggtg cgacgttcac gccgccttcg ccctggatgg gctcgacgag
     2941 cacggccgcc gtctgcgggc ccaccatcgc cttcgccgct tcgaggtcgc cgaatggagc
     3001 gtggaccatt ccgggcagca tcgggccgaa gccttcctgg agcgctggct gcgccgtcgc
     3061 cgtgagcccg ccgtaggtac ggccgtgaaa ggacttcgag aacgtcagga cctcgtgctt
     3121 gttgggaccg aaagtatcgt gggcgtactt gcgcgagagt ttgatcgccg cctccgcgct
     3181 ctcggcgccg gagttgcaga agaagaccct gtcgaagtcc gtggcgcccg tcagcatgcc
     3241 tgcgacctcg gcctggagcg gtatctcgta aaggttggag cagtggatta atttacctgc
     3301 ctgctcctgg atagccccga cgagcgcggg atgaccgtga cccagagaat tcgtcgcgat
     3361 cccacctat
//
LOCUS       DATAIR010000113         8033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_99251, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000113 DATAIR010000000
VERSION     DATAIR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99251"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VHM69_02875"
     CDS             <1..591
                     /locus_tag="VHM69_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739365.1"
                     /translation="GTIGNCVLLLRGWTDPAIGALPEKPTGTLPADVQRAIATIYAEQ
                     TDTTTQAQLQQEYIWYSIRIRLSLGLHGPTIDDVLDKGGYWGKGTALYTTALADIALP
                     EPTMGAVVNIRSIVLGDWDTAMPADAGPFYFHWQAGKTAEPCFARKGDEKACAWEYAS
                     ASGIAGSVATADGWHHKIPGDTPAPRGRIGSMYIYK"
     gene            2407..3063
                     /locus_tag="VHM69_02880"
     CDS             2407..3063
                     /locus_tag="VHM69_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739366.1"
                     /translation="MTSFPGSPRLLKGAIVGLDPANPLASVIVFQYNPDSVTRRLDAR
                     TSGGGDGADRSEAFRLTGPPKETVTLSVEVDAADQLEEANPLAVASGVYPALSALEML
                     LYPKSRTVIANAALARLGNIEIIPPEAPMTLFIWGTQRVVPVRLTSFSVTEEAYDTTL
                     NPIRARVELSLQILSYADLKQGHPGYNLFLAHQIVKEIMATTNVLNSAQNIGASLKLF
                     "
     gene            3072..3368
                     /locus_tag="VHM69_02885"
     CDS             3072..3368
                     /locus_tag="VHM69_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739367.1"
                     /translation="MFPTTSRYHEIETAKYTTPEGRQYVYLRRRFLPATAPATVLAEH
                     VVAQGERLDNITALYLGDPGQFWRLCDANGAMHPDELTAEVGRRLRIPMPGVGP"
     gene            3368..4495
                     /locus_tag="VHM69_02890"
     CDS             3368..4495
                     /locus_tag="VHM69_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739368.1"
                     /translation="MVDLGIRMQLLIGPTLPRPAPFDVVDALMELEVENKDRDARDGF
                     QMLFSLGKDSSLDYGLLREGFFDPPNRVVIMVFVGISAQVLIDGVITHHQVIPSNTPG
                     ESTLYVTGRDIGLQLDLEEKNATFKNMSDSAIVEKIIGSPEYVAYGLKPQVTRTTEVP
                     VETSRIRTQQGTDLAYIQELAGQNGFVFYIEPTLVPGVNTAYWGVENRGRPAQPPLSM
                     NMGPDTNVDSPITFSLDALGPAAPQVTIVEPLTRTAIPIPVPSSPLPSLTGRPATPLR
                     RTIPRNAANLDTVQALLQAVSVVSASSSDAVTANGQVDAVRYGRVLQSRRLVGVRGVG
                     SSYNGEYYVTQVKHRIKRGQYTQSFQLRREGRGATSPTVTT"
     gene            4492..4983
                     /locus_tag="VHM69_02895"
     CDS             4492..4983
                     /locus_tag="VHM69_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX2739369.1"
                     /translation="MNGRPFYGKYRGVVTDNRDPLQLGRVRARVQDVLGDQESGWAMP
                     AVPYAGKNVGLFLIPPVDALVWFEFEHGDPDYPIWTGCFWATGELPVLPAVAEKKVLK
                     TDVGAITLDDTPGAGGITIETAVGMKIQINAQGIEITNGQGGTVKLSGPQVSVNNGAL
                     EVT"
     gene            4983..5306
                     /locus_tag="VHM69_02900"
     CDS             4983..5306
                     /locus_tag="VHM69_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739370.1"
                     /translation="MPGFLLHAGATVLCLHAGQAQPVVSNPRVKVGGQPTVTQPSTYT
                     VAGCTLPPPIAANGPCVTAQWITAATRVRSGGVPLLLQDSQAICAPTGTGLNVVVTQV
                     RARGT"
     gene            5306..5665
                     /locus_tag="VHM69_02905"
     CDS             5306..5665
                     /locus_tag="VHM69_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX2739371.1"
                     /translation="MNIDFPYHFEGRGRTASTDDDDHIRDMIEQLLFTTPGERVNRPD
                     FGSGLLGMVFEPNSPELAATLEFGMQAAVQRELSDLIELQALEVTSEEASLRVVVQYV
                     VRRTGEPRTETIERSGA"
     gene            5665..>8033
                     /locus_tag="VHM69_02910"
     CDS             5665..>8033
                     /locus_tag="VHM69_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739372.1"
                     /translation="MNDPVLNCETPSRRERVRKESLNGLDYLEVSDDQRTLTVFFLGK
                     APEVIEKQNVLVEGGRRVSGIRVVDLRIHREDEPDLDDWMDVVVDRPGDFSTYTLRVA
                     GLDEDGSPTEDPMPGFDPRYCRLEFSFKVGCPSDLDCKTQVTCPPPQREEPEIDYLAK
                     DYASFRTLILDRLALVMPDWQERHAPDLGIALVEVLAYVGDHLSYYQDAVATEAYLET
                     SRQRASVRRHARLVDYQMHEGCNARAWVCVNTSDDITLDPNDLAFATEHDDAPQSGTE
                     LDGRPGGRYEVFEPLTKPGIRLYLYAEHDEIHFYTWGDERCCLPRGATSATLEDGWTD
                     DQDEPSDKESASGSRERTLHLKEGDVLIFEEVIGPGTGEPADADPSHRHAVRLTRVET
                     GEDALFDQPILEIEWAPEDALPFPLCLSAVGPAPACELIENVSVARGNVVLADHGQTV
                     GPEDLGAVPEAETIEWCGDECHPLETVVIPRKFRPRLREVPQTFSQALSPDVPATDLL
                     KQDPREAMPGIELLGTRVAPEGAVDEQWTVRRDLLGSGSQDRHFVAEVDDEGRARLRF
                     GDGDLGWMPEAGTIFRATYRIGNGPAGNVGAEAITRLLFRRNRVSGVELRPRNPLPAR
                     GGTPPEPLTEVKLLAPHASRTDLRRAITAEDYARLAERHPRVQRAAAALHWTGSWYEV
                     LVAIDARSDTGPRQGLAASAATFVDAVRQTVERHLELEEFRISPGGEMAGRVIGILRE
                     LQESAERDEPPKRLAALVRESLPSLNEEHDRAVARDYERLAPWTGDIVAA"
BASE COUNT         1578 a         2613 c         2559 g         1283 t
ORIGIN      
        1 ggaacaatcg gaaactgcgt cctcctcctc agagggtgga cggatcccgc gatcggcgcg
       61 ctgcccgaga aaccaaccgg aacgttgccg gccgacgtgc agcgggccat cgccactatc
      121 tacgcggaac agacggacac cacaacccag gcacagctgc agcaagagta catctggtat
      181 tcgatcagga tacggctgtc gctcgggttg cacggcccga ccatcgacga cgtgctcgac
      241 aaaggcggct actggggcaa gggtacggca ctctacacga cggctctggc agacatcgcc
      301 ctaccagaac cgactatggg cgcggttgtg aacatcagga gcatcgtact gggtgattgg
      361 gacacggcga tgccggccga cgcaggcccc ttctacttcc actggcaggc aggtaagacg
      421 gctgaaccat gcttcgccag gaagggggac gagaaggcat gcgcctggga gtacgccagt
      481 gcatcaggaa tcgcaggttc tgttgccacg gcggacggtt ggcaccacaa gatccccggc
      541 gacacccctg cccccagggg ccgcataggc tccatgtaca tctacaagta gcgcaggagc
      601 gacgagttga aacagcttac gaaacccgga ccaaggcccc gaaaggcaac cgctcctact
      661 agatccattg ccggcaggcc gagcgcaagt ctcggaaaca gggggcaact agcgctacat
      721 cgcagagact cacacccggc cgaacctgat tcggtgccgc ccatagtcca tgaggtgctg
      781 cgctcgcccg gccggcccct ggacgccgct acgcgctcgt tcatggagcc gcgcttcggc
      841 cacgatttcg gcggagtgcg cgtgcacacg gacgcgaagg ccgctgagtc ggcgcggacg
      901 gtaatggcat cggcgtacac ggtggggcaa aaggtcgtct ttggtgctgg acagtatgcg
      961 ccggaggcaa gccgggggca gcatcttctg gctcatgagt tgacacacgt ggtgcaacag
     1021 aatcccatcg ccatcaggtg gtcaagcgat atcgagattg gtgaagcggg cacagctctg
     1081 gaactagagg cagacagaat ggccgacgct gtaataagta gggcgcagca gccatacagg
     1141 ccgacacgaa atgcccctgc catacttcag cgagcctgtc cacgaccacc gaccgggctc
     1201 ggcaacacta ttgcgtccga accgtgcgat ccaggcccac ccgactacgt ggctggcaaa
     1261 aacttgcagt tttgccagga ttcggatgaa ctaatggacg gccagacgac ttgggtgaac
     1321 gaagtgatag ccgatgcgcg acgtgccacg agcatcgttc tgcacggcaa cgcctctatg
     1381 gaggggccac cgggtgatta caatcgcaat cttgcctgca agcgggcggt tgctattgcc
     1441 actcggcttc gcgggagtgg tgtcaccact cccatgactc tggtttcgca tggtcctatt
     1501 agcgtctatg gatcagcaga ggctaaccgt aatgtcgttg ctgtcttgac cgtaccgtcg
     1561 cctcctccac cgcctccgcc cggaccatct gcaccagcgc ccgcggcctc tcagcatctc
     1621 gatcaacaga tctattgcgt gacgtatata cgcggcggct gcccccaaat tgtttccgga
     1681 ggggttcccg gcgaagaaga gattgcgggg cacgatacgc tttgccgggc tgacacaggt
     1741 tatgctggtc cgccggtgac actcgcagat cttcactgtg aggagcttgg cctcggtatt
     1801 gctctgagcc tcgagagaac tcatccgggt tggaagagcg cactcccacc ttgtccctgc
     1861 accgcgcaag cagcgagcat cgccggttgg agcacgtcca acacattctt gagcagcttt
     1921 catcctggtg cagcgacggc ttacaggcgc gacgcatccg ggtcgcacca tgcccaacag
     1981 tgttgctacg acgacagcct gggcagaggc atctctggcg gcaccggaga cggcctgccg
     2041 gatggacgcc tcatcacaga aggagctggt gcgggcacac ctgacttctg gtcgtcctca
     2101 agctccttct tcaaccatca acggatcgac gtcgagccgt ggcgatcgct gggatgggag
     2161 agatacaacc agtattggac ccccgataac gcgaatggct gtcctcgcaa tcgcaaacca
     2221 taggtctgga gtcgatagga tgagagcaca acgactacat cgcccagata cgtcgcttgg
     2281 gggcgcggac gcaaaatcaa cacttccggc gcaagctacg gttggttttg cagcgccgct
     2341 ccacggatca aacagaaccg ccgacatatc tgtccgtgca cgtatttgtg gagaggttgg
     2401 gttcccatga cctcgttccc cggctcacca cgtctgctca aaggcgccat agtcgggctg
     2461 gatccggcga accctctggc gagcgtgatc gtcttccagt acaacccgga ctcggtgacg
     2521 cggcgtcttg acgcgcgcac gtcgggtggc ggcgacggtg cagacaggtc cgaggccttc
     2581 cgcctgaccg gtcctccgaa ggagacggta acgctgagcg tcgaggtgga cgcagcggac
     2641 cagcttgagg aggcgaaccc gctggccgtg gcctccggcg tgtacccggc gctctccgcg
     2701 ctggagatgc tgctctaccc caagagccgg acggtcatcg ccaacgcggc gctcgcccgg
     2761 ctcggcaaca tcgagatcat cccgcccgag gcgccgatga cgctctttat ctggggtacg
     2821 cagcgggtcg tgccggtgcg gctgacgagc ttcagcgtca cggaggaggc atatgacact
     2881 acattgaacc cgatccgcgc cagggtcgag ttgagcctgc agatccttag ctatgcggac
     2941 ctgaagcagg gccatccagg ctacaacctc ttcctggcgc accagatcgt caaagagatc
     3001 atggcgacga ccaacgtact aaatagcgcg cagaacatcg gcgcgtcgct gaagctgttt
     3061 tgaggtgaga gatgtttcct acgaccagcc gctaccacga gatcgagacc gcaaagtaca
     3121 ccaccccgga agggagacag tacgtctacc tgcgccgccg cttcctgccc gccacagcac
     3181 cggcgaccgt tctggccgag cacgtcgtcg cccagggcga gaggctcgac aacatcaccg
     3241 ccctctacct cggcgaccct gggcagttct ggaggctgtg cgatgccaac ggggcgatgc
     3301 atcccgatga gctgaccgcc gaggtcggac ggcgcctgcg gataccgatg ccgggagtgg
     3361 gtccatagtg gtcgatctcg gcattcgcat gcagctcctg atcgggccta ccctcccgcg
     3421 cccggcaccc ttcgacgtgg tggacgccct catggagctc gaggtcgaga acaaagaccg
     3481 cgacgcgcgc gacgggttcc agatgctttt cagcctgggc aaggactcct cgctggacta
     3541 cgggctgttg cgcgaaggtt tcttcgaccc gccaaacagg gttgtcataa tggtcttcgt
     3601 cggcatatcg gcccaggtgc tcatcgacgg cgtaatcacc caccatcagg tcatccccag
     3661 caacaccccg ggcgaatcga ccctgtatgt cacgggcagg gatatcggcc tccagcttga
     3721 cctggaggag aagaatgcca ccttcaagaa catgtccgac tcggccatcg tcgagaagat
     3781 catcggctcg cccgaatacg ttgcctacgg gctgaagccc caggtcacac gcacgaccga
     3841 ggtgccggtt gagacgagcc gtatccggac tcagcagggc accgacctcg catacatcca
     3901 ggaactcgcc gggcaaaacg gattcgtctt ctacatcgag ccaaccctgg ttccaggtgt
     3961 caacaccgcc tactggggag tggagaaccg gggtaggccg gcccagcccc cgctgtcgat
     4021 gaacatgggg cctgacacca acgtggactc cccgatcacc ttcagcctcg acgcgcttgg
     4081 tccggcagca ccccaggtga ccattgtcga gccgctgacc aggacggcga tcccgatccc
     4141 cgtccctagc agtccgctgc cttcgctcac gggccggccg gccactccgc tacgcaggac
     4201 cataccccgg aacgccgcca acctcgacac cgtgcaggcc ctgctgcaag ccgtctccgt
     4261 ggtcagcgcg tcgtcctcgg acgcggtgac cgcaaacggg caggtggacg cggtgcgcta
     4321 cggccgtgtc ctgcagtccc gcaggctggt aggggtcagg ggcgtcggct caagttacaa
     4381 cggagagtac tacgtgacac aggtgaagca ccgcatcaag cgcggccagt acacgcagag
     4441 cttccagctc cgccgcgaag gacgcggcgc gacgtccccg acggtgacga cgtgaacggg
     4501 cgaccgttct acggcaagta ccgcggggtc gtcactgaca accgcgaccc tttgcaactg
     4561 ggacgggtgc gggccagggt gcaggacgtg cttggggatc aggagagcgg ttgggcaatg
     4621 cccgccgtgc cttacgcggg caagaacgtc gggctcttcc tgatcccacc cgtggacgcg
     4681 ctcgtctggt tcgagttcga gcacggcgac cccgactacc ccatctggac gggttgcttc
     4741 tgggcgacgg gcgagctgcc tgtgctgccc gccgtagccg agaagaaggt cctcaagacc
     4801 gacgtcggcg ccataaccct ggacgacacg ccgggcgctg gcggcatcac catcgagacc
     4861 gcggtcggaa tgaagatcca gatcaacgcg caggggatcg agatcacgaa cggccagggc
     4921 ggcacggtga agctctcggg cccgcaggtg tccgtaaata acggtgccct ggaggtgacc
     4981 tgatgcctgg tttcctgctc cacgccggcg cgacggtgct gtgcctgcac gccgggcagg
     5041 ctcagccggt tgtttcgaac ccgcgggtga aggtgggcgg ccagccgacc gtcacgcaac
     5101 cgagcacgta tacggtcgcc ggttgtaccc tgcccccgcc catcgccgcc aacggtccct
     5161 gcgtcacggc gcagtggatc acggcggcca cgagggtgag gtcggggggc gtacctcttc
     5221 tgctgcagga cagccaggcg atctgtgccc ccactggcac agggttgaat gtcgtcgtta
     5281 cgcaggtacg cgcgagagga acgtgatgaa catcgacttc ccataccatt tcgaaggccg
     5341 gggccgaacg gcctccaccg acgatgacga ccacatccgc gacatgatcg agcaactcct
     5401 cttcacgacc ccgggagagc gggtcaacag gccggacttc ggcagcggcc tgctcgggat
     5461 ggtcttcgag cccaacagcc ccgagctggc ggcgaccctg gagtttggaa tgcaggcagc
     5521 cgtccagcgc gagttgagcg acctgatcga gctgcaggcg ctggaggtga ccagcgagga
     5581 agcgagcctg cgcgtggtgg tgcagtacgt ggtgcgccga accggagagc cgcgtaccga
     5641 gacgatcgaa cggagcggcg cgtgatgaac gacccagttt tgaactgcga gacaccgagt
     5701 cgccgcgaga gggtcaggaa ggaaagcctg aacggactgg attacctgga ggtgagcgac
     5761 gatcaacgca cgttgaccgt cttcttcctg ggcaaagcgc cggaggtgat cgagaagcag
     5821 aacgtgctcg tggagggtgg gcgacgcgtc agtggcatca gggtcgtcga ccttcgtata
     5881 cacagggagg acgagcccga cctcgacgac tggatggacg tcgtcgttga ccgccctggc
     5941 gacttctcga cctacaccct gcgggtggcc gggctggacg aggacggcag tccgacggag
     6001 gacccgatgc ctggcttcga tccccgctac tgcagactgg agttcagctt caaggtggga
     6061 tgccccagcg accttgactg caagacgcaa gtgacgtgtc ccccaccgca acgcgaagag
     6121 cccgagatcg actacctggc caaggactac gcctccttcc gaaccctgat cctggaccgc
     6181 ctggcgctcg tgatgcccga ctggcaggaa cgtcacgccc ctgacctcgg catagccctc
     6241 gtcgaggtcc tcgcctacgt cggagatcac ctcagctact accaggacgc cgtcgccaca
     6301 gaggcttacc tggagacatc ccgccagcgc gcctcggtac gccgccacgc gcggctggtc
     6361 gattaccaga tgcacgaggg atgcaatgct cgcgcgtggg tctgcgtgaa taccagcgac
     6421 gacatcacac tcgacccgaa cgacttagca ttcgctaccg aacacgacga cgccccgcaa
     6481 agcggcacgg agctcgacgg gcgtcccggt ggccgctacg aggtcttcga gccgctgacc
     6541 aagccgggca tacgactgta cctgtacgcg gagcacgacg agatccactt ctatacctgg
     6601 ggcgacgagc gatgctgcct gccccgcggc gcaacctcgg cgacgctaga ggacggatgg
     6661 accgatgacc aggatgaacc ctcggataaa gaatctgcgt ccggttcgcg cgaacgaacc
     6721 ctgcacctga aggaagggga cgtcctgatc ttcgaggagg tcatcggccc tgggacgggc
     6781 gaacccgccg atgccgaccc ctcccaccgc cacgccgtac gccttacccg cgtggagacc
     6841 ggcgaggacg ctctcttcga ccagccgatc ctggagatag agtgggcgcc cgaggacgct
     6901 cttcccttcc cactctgcct gtccgccgtc ggtcccgctc ccgcctgcga gctcatcgag
     6961 aacgtcagcg tcgcgcgggg caacgtcgtg ctggccgacc acggtcagac ggtgggtccc
     7021 gaggaccttg gggccgtacc ggaggcagag acgatcgaat ggtgcgggga cgaatgccac
     7081 cccctcgaga ccgttgtgat accccgcaag ttcaggcccc gcctgcggga ggtaccccag
     7141 accttcagcc aggcgctttc gcccgacgtc cctgcgaccg accttctgaa gcaggaccca
     7201 cgcgaggcca tgcccgggat cgagctcctc ggcacccgcg tcgcacctga aggcgccgtc
     7261 gacgagcagt ggacggtaag gcgagatctg ctgggaagcg gtagccagga ccgccatttc
     7321 gtggccgagg tggacgacga agggcgggca aggctgcgtt tcggcgacgg agatctgggg
     7381 tggatgcccg aggccggcac catcttcagg gcgacgtacc ggattggaaa cggtcctgcc
     7441 ggcaacgtgg gggccgaagc cattacccgc ctcctcttcc gccgcaacag ggtaagcggc
     7501 gtcgagttgc ggcctcgcaa cccgctcccc gcccggggcg gcacgcctcc ggaaccgctg
     7561 accgaggtga agctgctcgc gccgcacgct tcccggaccg acttgcggcg cgccatcacc
     7621 gccgaagact acgcccgcct cgcagaacgc caccccaggg tacagcgtgc ggcggccgcg
     7681 ctgcactgga cagggagctg gtacgaggtg ctggtggcga tcgacgcgcg cagcgacacc
     7741 ggaccccggc aggggctcgc agcctcagcg gccaccttcg tggacgccgt gcgccagacg
     7801 gtggaacgac acctggaatt ggaggagttc cgcatctctc ccggcggaga gatggcaggc
     7861 agggtcatcg gcatcctccg ggaactgcaa gagagcgcgg agcgcgacga gccacccaaa
     7921 cgcctcgccg cgctggtgcg tgagagtcta ccgtcgctga acgaggagca cgaccgcgcc
     7981 gtcgccaggg actacgaaag gcttgcgccg tggacgggcg acatagtggc cgc
//
LOCUS       DATAIR010000114         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_99261, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000114 DATAIR010000000
VERSION     DATAIR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99261"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VHM69_02915"
     CDS             <1..537
                     /locus_tag="VHM69_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739373.1"
                     /translation="PRLLTSLWFIGLSPLLFSGLAVLVPLELYSLGWGAAAVGTVFLI
                     SAAFEALVHPLLGKWSDRSGYRPPVYSGLLASIGILLLLTWVGNPWIIGLLVVLAAGA
                     FNATLVPGTALFSRSTEKAGVEGAVAFAITNFAWASGYAVGAPLGGFLADLRGDALSY
                     LSLTAVCLLTLFLLYRTA"
     gene            600..1304
                     /locus_tag="VHM69_02920"
     CDS             600..1304
                     /locus_tag="VHM69_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX2739374.1"
                     /translation="MAQPIDLYYWPTPNGWKITIFLEETNLPYNIVPVDIAAGAQYEP
                     EFLEISPNNKIPAIIDPEGPDGEPISVAESGAILIYLAEKTGRFLPETPRERYLVLQW
                     LMFQMGHVGPMLGQAHHFRGYAPEKIPYAIQRYTDEAARLYRVIDGQLSETDYVAGDE
                     YTIADMAIFPWLVSHEKQGQDLDDYPNLKRWYEGMESRPAVNRAREVGKELRRSLENM
                     DDKTRQMLFGGGPKRD"
BASE COUNT          302 a          504 c          455 g          277 t
ORIGIN      
        1 ccgaggctcc tgacaagcct ctggtttata gggctctctc ccctgctctt cagcggtctt
       61 gccgtgctcg tcccactcga gttgtacagt ctcggatggg gcgctgctgc ggtggggacg
      121 gtcttcctga ttagcgcggc cttcgaggct cttgtacacc cgctactcgg caaatggtcc
      181 gaccgctccg gctaccgccc gcccgtgtac tccggtctgc tcgcctccat cggcatcctg
      241 ctcctcctga cgtgggtcgg gaacccgtgg atcatcggac ttctcgtcgt gcttgcggcg
      301 ggagctttca acgcgacgct cgtcccgggg acggcgctct tctcccgcag caccgagaag
      361 gcgggggtgg aaggggccgt tgccttcgcc ataacgaact tcgcctgggc atccggttac
      421 gccgtcgggg cacctctggg tggcttcctg gcagacctca ggggcgacgc cctctcctac
      481 ctctcgctta cggcggtctg cctgttgacc ctctttctac tctaccggac ggcttgacgg
      541 agccaggttc tcctatactg taacttcata gtggtatacc cggcgctagg agaaggctga
      601 tggcgcaacc gatcgatctc tactactggc cgacaccaaa cggatggaag atcacgatct
      661 tcctcgaaga aaccaacctc ccttacaaca tcgtgcccgt ggacatcgcg gccggcgctc
      721 agtacgagcc ggagttcctg gagatcagcc caaacaacaa gatccccgct atcatcgacc
      781 ccgaagggcc ggacggagag ccgatctccg tcgccgagtc gggggccatc ctgatctacc
      841 tcgcagagaa aacgggccgc ttcctacccg aaaccccgcg cgagcgctac ctggtgttgc
      901 aatggctgat gttccagatg gggcacgtcg ggccgatgct cggccaggcc caccatttcc
      961 gcggctacgc ccccgagaag atcccctacg caatccaacg ttacaccgac gaggccgcgc
     1021 ggctctacag ggtaattgac ggccaactct cggagacaga ttacgtcgca ggcgacgagt
     1081 acacgatagc ggacatggcg atcttcccct ggctcgtctc ccacgagaag cagggccagg
     1141 acctcgacga ttacccgaac ctgaagcgct ggtacgaggg catggagtca cgccccgcag
     1201 tcaaccgcgc gagggaggtc ggcaaggagc tacgccgctc gctggaaaac atggacgaca
     1261 agacccgcca gatgctcttc gggggcggac cgaagaggga ttaaacgctg accggcacgc
     1321 tgtgccttcc gcttgtcagc acttggggcg agggagaaaa gcttctctca accaagatgc
     1381 atacgactct acgtccaatc taatgatcgc gcagggcgat acggtatccg acggcgagca
     1441 gaggcgcgcc ccagaggatc acggatacga cgacaagaca gactccgggt accaggaagg
     1501 gggcgccgaa tccgaaggac gcggcaagca atccacct
//
LOCUS       DATAIR010000115         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_326180, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000115 DATAIR010000000
VERSION     DATAIR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326180"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="VHM69_02925"
     CDS             complement(<1..1923)
                     /locus_tag="VHM69_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX2739375.1"
                     /translation="MILERLFIENYKQLRDPLELYPPEGAIGIVGPNGAGKSTLFESI
                     LWAFFGSRGGGPRFANESIPWSGGTTKDPTTVEVTLATEGGSYTVRRRLKSGSTTAEA
                     LDAAGRTVVSGTADVTRWVEENLLRMDRTAFEATFYARQKELRFFANDEGIGRVRKIS
                     KLLGISSVETAQSFLREDRNTLRAEARLLEGRLAEADLDSLQTELKTASTECDRLEAE
                     LEKVSEEYEVAERDLKAAREARAGLEITYRQHSRLTGDLREAESEKRRSADRRAGAEK
                     DLADLVAAEQELGRLRPQTARLPEVAAELERLDEDKRRSEQRDRDRREYMQAQRRISD
                     IEGEVSDELEELDGDEVLPGFLALFDMDGLELLSETLSVLERAAGELKKAEARHDGLR
                     RLAEDHTSYQRAAQAVREADGLYQETRMEAARLAEELDDLSGGEDLERAERELREQEE
                     KLRELAALHRGRANADEREAKNVDKAREAIDSGAEEHCPTCHREFESGEQAEISDTLN
                     RQAAAIRRRADRETEEARKLAASADETAEKLKKVTSNLNRWRGLRETKVRAEDRAAGR
                     LETLEKARSTLEEIEAGIAGKQAPTEADLEVASARCDRLRELRDVRPHMKSLATEQSK
                     LTQRVAELADELEALSG"
     gene            complement(1920..>2303)
                     /locus_tag="VHM69_02930"
     CDS             complement(1920..>2303)
                     /locus_tag="VHM69_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739376.1"
                     /translation="RDMDAAALTEAVRERTSGVDLDGAIVRLRAYDVRHGVASGMDRE
                     LLRDLQRRCLNFSLEVHAEERPEDLEKGASSNAVFGPLGEEFAAFVSQRKERGELEAK
                     FAEEFLEKGRAYLTRAASEEPESAA"
BASE COUNT          294 a          810 c          725 g          474 t
ORIGIN      
        1 gcccgagagc gcttcgagct cgtcagcgag ctcggcgacg cgctgcgtga gcttcgactg
       61 ctcggttgcc aggctcttca tgtgcggacg gacgtcgcgc aactctctca aacggtcgca
      121 gcgggcgctg gccacttcga ggtcggcttc tgttggagct tgcttgcctg ctataccggc
      181 ttcgatctcc tcgagggtgg agcgggcttt ctcgagcgtt tcgagacggc ctgcggctcg
      241 gtcctcggca cggaccttcg tctcgcgcag cccgcgccag cggttcaggt tcgatgtgac
      301 ctttttcagc ttctcggcgg tctcgtccgc ggaggcggcg agctttctgg cctcttcagt
      361 ttcacggtcg gcgcggcgac ggatcgcggc cgcctgccta ttgagggtgt cggagatctc
      421 cgcctgctcc ccgctctcga actccctgtg gcaggtgggg cagtgctcct cggcgccgct
      481 gtcgatggct tctctggcct tgtcgacgtt cttcgcctcg cgctcgtccg cgttggcgcg
      541 gcccctgtgg agcgcggcaa gttcccgcag tttctcctcc tgctcacgca gctcgcgctc
      601 ggctcgctcc aggtcctcgc cgcccgagag gtcatccagc tcctcggcga gccgggctgc
      661 ttccatccgc gtctcctgat acagcccgtc ggcctcgcgg acggcttgcg cggcgcgctg
      721 gtaggaggtg tgatcctcgg cgagcctccg cagcccatcg tggcgagcct ccgctttctt
      781 caactcccca gcggcccgct cgagtaccga cagggtctcc gagaggagct caaggccgtc
      841 catatcgaat agcgcgagga agcctggcag gacctcgtcc ccgtccagct cttccagttc
      901 gtcggagacc tcgccctcga tgtccgagat gcgccgctgt gcctgcatgt attccctgcg
      961 gtcgcggtcg cgctgctcgg agcggcgctt gtcctcgtcc agcctctcca actccgccgc
     1021 tacttccgga agcctggcgg tctggggcct caggcgaccc agctcctgtt cggcggcgac
     1081 caggtcggcg aggtccttct cggccccagc acggcggtcg gcagaccggc gcttctcgga
     1141 ctccgcctcg cgcaggtcgc ccgtgaggcg cgagtgttgg cggtaggtga tctccaatcc
     1201 ggcgcgtgcc tcgcgggcgg ctttgagatc cctctcggcc acttcgtact cttctgagac
     1261 cttctccaac tcggcttcga ggcggtcgca ctcggtgctg gcggttttga gctcggtctg
     1321 gagagagtcg aggtcggctt ctgcgaggcg gccttcgagg agccgcgctt cggctcggag
     1381 agtattgcgg tcctcgcgca agaaagactg ggcggtctcg acgctgctga taccgaggag
     1441 tttggagatc tttcgcaccc tgcctatgcc ttcgtcgttg gcgaagaaac gaagctcctt
     1501 ctgcctggcg tagaacgtcg cctcgaaggc cgtcctgtcc atcctcagca ggttctcctc
     1561 gacccagcgc gtgacgtcgg ctgtcccgct gacgaccgtc ctaccagcgg cgtcgagcgc
     1621 ctctgccgtc gtcgaaccgc tcttgaggcg ccgccggacc gtataagatc ctccttccgt
     1681 cgccagggtc acctcgaccg tcgtcgggtc cttcgtcgtt ccgccgctcc aggggatgga
     1741 ctcgttcgcg aagcgcggtc cgccgccgcg cgatccgaag aaggcccaca ggatgctctc
     1801 gaagagggtt gacttgccag cgccgttcgg cccgacgata ccgatggctc cttcgggggg
     1861 atacagctcc aggggatccc tcagctgctt gtagttctcg ataaagaggc gctccaggat
     1921 cacgcggcgc tctccggttc ttcgctcgcc gcccgcgtca ggtatgcgcg tcccttctcc
     1981 agaaattctt cggcgaactt cgcttccagt tccccgcgtt ccttgcgctg ggagacgaac
     2041 gccgcgaact cctcaccaag gggtccgaag acggcgttcg aggaggcgcc cttctccaga
     2101 tcctccggcc tttcctcggc gtggacctcg agcgagaagt tcaggcaccg ccgttgcaga
     2161 tcccgcagca gctcgcggtc catcccgctc gctacgccgt gccgcacgtc gtaggcgcgg
     2221 aggcggacga tcgcaccgtc caagtcaacc ccggaagttc gctcgcgcac cgcctccgtc
     2281 aacgctgcgg cgtccatgtc gcg
//
LOCUS       DATAIR010000116         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_49260, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000116 DATAIR010000000
VERSION     DATAIR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49260"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..668
                     /locus_tag="VHM69_02935"
     CDS             141..668
                     /locus_tag="VHM69_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739377.1"
                     /translation="MRRAMILLATIATAVLVAAGVALAVEEIGTNSGETLRGTGNRDV
                     LYGRGGNDKLLGRGGNDELLAGSGNDTVQGSSGADELYGTSGADSMNGGDDNDGVYGG
                     VGDDPAIFGGGGTDDISGGWGDDTLNSRDNLVDSVSCGPNQDVANVDDDPSTPADPDD
                     SIDKVSPDCEDVNPG"
     gene            complement(702..1691)
                     /gene="tsaD"
                     /locus_tag="VHM69_02940"
     CDS             complement(702..1691)
                     /gene="tsaD"
                     /locus_tag="VHM69_02940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2739378.1"
                     /translation="MILAIETSCDDTCAAVVEPDGRRALSNVVHTQTEHARYGGVVPE
                     VASRAHLERLDGVVEKSLSDADISLDEVSRVAVTVRPGLIGALLVGVAAAKSLAFARA
                     LPLVPVNHLEGHVAAAYLSDPGLQPPLVALIASGGHTALYSVDPDRKMRLLGETLDDA
                     AGEALDKGARMLGLGFPGGPAISRAARDGDRDRYDFPVGLKGKDNLDFSFSGLKTSLL
                     YKLKAMDAEKVREELPHLAASYEAAVVEALSRKLLRAADIHDAPAVVVAGGVAANAFL
                     RSTLEDECAKLGTDLVIPPPELCTDNAAMIGAAAPHSTALAYPEYLSLNARSV"
     gene            2254..2850
                     /locus_tag="VHM69_02945"
     CDS             2254..2850
                     /locus_tag="VHM69_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739379.1"
                     /translation="MRRVILVAVICAVLIGVAVFLIGAPKTSAGGGEDDRVVGTAQSD
                     TRFGGSGVDLMVGRAGDDRLYGEANVDGLFGRTGDDTLDTGGVGGTANGGPGEDELYG
                     GYGLDRLDGSSGDDRISSAAGDDRLEGGLGNDTIRGEGGNDSLFGESGRDTMFGGDGD
                     DFIYAIDGTPGETVDCGPGANDMASADEGDVVVNCEIN"
     gene            2924..>4504
                     /locus_tag="VHM69_02950"
     CDS             2924..>4504
                     /locus_tag="VHM69_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2739380.1"
                     /translation="MDRAGAELTINPTTALWRSGPTATRVAWFMWVLCLALVASSLLL
                     DILTPDFLAPSERPDFVLALSTVLLSLACPTVGALVVSRLPANPVGWIFCGMGLLYGV
                     RRFAVAYADHALLYRPWLPGGEYAAWVSTWLGFSGLAALGVLLVMLFPEGRSLSRRWR
                     ALAWVAVCGAVMVGLGDALRFGPLQGYYYVRNPVGVAGTTVGALPTYRLFEASIFVGG
                     TLLSVGCLACVISLILRLRRSGGEVRQQLNWFACAAVPAVIGATVILVDRTIEEFTLL
                     FLDTPVRPALRVARNFEFLVRDDKSLGPLVDLRLETTFEFLAALALFIVPIFTGVAIL
                     RHHLYHIDLVINRTLVFGALTAVVISLYVFLVAIFGALFQMGSGDNLVVPLLATALIA
                     VLFQPLRERLQRSVNRLMYGERDDPYIALSRLGQRLEATMAPEVVLPTIVETVAGALK
                     VPYAAIELKQDESYRTAAAHGSPAGEPTVVPLVRHNQEVGRLVLSPRTPNESFSPADR
                     LLLQDLARQAGVAVHAVRL"
BASE COUNT          766 a         1375 c         1490 g          873 t
ORIGIN      
        1 tggtcaaata tccgactcgc gacgttctga gagaagacct ttgtgactat aaatgtagtt
       61 cttttggacg atgtgccgtc ccatgtacgc ccgctatcgt ggtgtcgtta gaaggtgcag
      121 tagacggaag gacgaagaag atgagaagag cgatgatctt gcttgcgacg attgcaacgg
      181 cggttctggt ggccgccggg gtcgctctcg cggtcgagga gataggcaca aactcgggag
      241 agacgctcag gggcacaggc aatcgcgacg tcctctacgg caggggtggc aacgacaagc
      301 tgctcgggcg gggaggaaac gacgagctac tggcaggttc tggcaacgat accgtgcaag
      361 ggtcttcagg ggccgacgag ctctacggca cctctggcgc cgactccatg aacggcgggg
      421 acgacaacga cggggtctac ggtggggttg gagacgatcc tgcgatcttc ggtggtggtg
      481 gcaccgacga tatctcgggc ggctggggcg acgacacact aaacagccgc gacaacttgg
      541 tcgactcggt cagctgtggt ccaaaccagg atgtcgccaa cgtggacgac gatccgtcca
      601 ctcctgcgga tccagatgac tcgatcgaca aagtctcccc ggactgcgag gacgttaacc
      661 ccgggtgagg gtggctttac ccacctgcct tccggcaccc ctcagacgct tctggcgttc
      721 aggctcaggt actcggggta ggcgagggcg gtcgagtggg gtgctgcggc cccgatcatc
      781 gcggcgttgt ccgtgcacaa ctcgggcggc ggtatgacga ggtcggttcc gagcttcgcg
      841 cattcatctt cgagggtgct gcggaggaag gcgttggctg cgactccccc tgccacgacg
      901 accgcgggcg cgtcgtggat gtccgcggcg cggaggagct tgcgggagag ggcctcgacg
      961 acggcagcct cgtagctcgc tgccaggtgc ggcagctcct cgcggacctt ctccgcgtcc
     1021 atcgccttca gcttgtacag gaggctcgtc ttgaggcctg agaagctgaa gtccaggttg
     1081 tccttgccct tgaggccgac cgggaaatcg tagcggtcac ggtctccgtc gcgcgcggct
     1141 ctggaaatcg caggtccgcc ggggaagccg aggcccagca tcctggctcc cttgtccagg
     1201 gcctcgcccg cggcgtcgtc gagggtctcg ccgaggaggc gcatcttgcg gtcggggtcc
     1261 acggagtaga gggccgtatg gccgccagag gcgatcaagg ctaccaacgg cggctgcaat
     1321 ccgggatctg agaggtacgc ggctgcaacg tggccttcga ggtggttcac cgggactagc
     1381 gggagcgcgc gggcaaacgc gaggctcttc gcggccgcga ccccgacgag cagagccccg
     1441 atcaaacccg gcctgaccgt caccgcgacg cggctgactt cgtcaagaga gatatcagcg
     1501 tcagacagag acttctcaac gaccccgtcg aggcgttcga ggtgggcgcg agaggcgacc
     1561 tcaggtacga cgccgccgta gcgcgcgtgc tcggtctgcg tatggacgac gttcgagagg
     1621 gcgcgtcttc cgtcgggctc cacgacggcg gcgcaggtgt catcgcagga agtctctatc
     1681 gccaggatca caggctgttt atagcacttg cgcccactac ggccgcccga tcacctatgc
     1741 tgccacgatg gtgtgtggca ccaacatggc ggtagatacc ggagcccgcc ttcttgttct
     1801 ggatatctct ccggcggcag aaggacgctc gcggaagcca cgcccagttt tccgcgcacc
     1861 gcgaactcga tcatggctgg cctggttttt cgatgctccg atgatactcc gggagatcag
     1921 ccgtagtcgt gggacggtgg tcccgttttg tcatgagact gtgatcccat gacctcttgc
     1981 ataccggata gagtacgttg ccccggcaca cacggcagta cgtggcgaaa ggctggcgag
     2041 accccctggt tctctggcgc atcgagatat tcgctaccgt gggttacgga gtagactgtg
     2101 gatcaagcaa ggtcaccgac tgccgcgaag gacagtgatc gggagcccat cgccaactat
     2161 ggaaaaccag cacggcgcgc gcgatcacgt ttcgaacgca tcatccctag gtggatgggc
     2221 caacggtcct gagaaacctg aggggaagat gtgatgagaa gggtcatctt ggttgcggtg
     2281 atctgtgcgg tcctcatcgg ggttgccgtg ttcctgatcg gcgcgccgaa gacttcggca
     2341 ggtggcggcg aggacgaccg ggtggtcggc accgcccaat ccgacaccag gttcggcggt
     2401 tccggcgtcg acctgatggt ggggagagca ggcgacgacc gtctatacgg cgaagcaaac
     2461 gttgatggct tgttcggccg caccggcgac gacactcttg acaccggagg cgtcgggggc
     2521 acggccaacg gtgggccggg cgaagatgaa ctttacggcg gatacggtct ggatcgcctg
     2581 gacggctcgt cgggtgacga caggatcagc agtgccgctg gcgacgaccg tttggaggga
     2641 ggactgggca acgataccat caggggcgag ggtggcaacg actcgctctt cggagagtcg
     2701 ggccgagata caatgttcgg cggtgacggc gacgacttta tctacgcgat cgacggcacc
     2761 ccgggcgaga ccgtcgattg cggccctgga gccaacgaca tggcctccgc tgatgaagga
     2821 gacgtagtgg tgaactgcga gatcaactga ctgccagagg gccgatctat accttcgtgt
     2881 accctgatag gaagaagagt cgtagccagg agacgggcca gtcgtggaca gagccggcgc
     2941 ggaactgaca attaatccga cgacggcgtt gtggaggagc ggtcctaccg ccactcgggt
     3001 agcctggttc atgtgggtac tgtgcctggc gctcgtcgct tcgagtctcc ttctggacat
     3061 actcaccccc gacttcctcg ccccctccga gagacccgac ttcgttctcg ccctctccac
     3121 ggtcctgttg tcgctggctt gcccgacggt tggcgccctg gtggtctcgc gcctccccgc
     3181 gaacccggta ggttggatct tctgtggcat gggcttgctt tacggggtgc ggcgtttcgc
     3241 ggtggcgtac gcagaccacg cattgctcta ccggccctgg ttgcctggag gagagtatgc
     3301 agcgtgggtc tccacgtggc tcggattctc gggcctcgct gcgttgggcg tgctccttgt
     3361 catgttattc cctgaaggcc ggtcgttgtc ccgacgctgg cgagccctgg cgtgggtagc
     3421 cgtctgcggg gccgtcatgg tcgggctcgg agacgcacta cggttcggtc cccttcaagg
     3481 ctactactac gttcgcaacc cggtcggggt ggccggcacg accgtgggcg cgctgcccac
     3541 gtacaggctc ttcgaagcgt ccatcttcgt tggcgggacg ctgctctcgg tggggtgcct
     3601 ggcctgcgtc atctccctga tactgcgtct caggcggtcc gggggcgaag tgcgtcagca
     3661 gctcaactgg ttcgcctgcg ccgcagttcc cgcggtaatc ggtgcgaccg tgatcctcgt
     3721 ggacaggacc atcgaagagt tcacgctgct ctttcttgac actccggttc gtccggcgct
     3781 tcgtgtggcc aggaacttcg agttcctcgt cagggacgac aaatcgcttg gcccccttgt
     3841 cgacttgcgg cttgagacga ccttcgagtt tctggccgcg ctcgccctct tcatcgttcc
     3901 catcttcacg ggcgtcgcga tactgcgcca ccatctctac cacatagacc tggtcatcaa
     3961 ccgtaccctc gtcttcggcg cgctgacggc tgttgtgatc tctctctacg tcttcctcgt
     4021 cgccatcttc ggcgcgctct tccagatggg atcgggggac aacctggtcg tccccctcct
     4081 ggctacggct ttgatcgccg tcctctttca gcccctgcgc gagcgccttc agcgcagcgt
     4141 gaatcgtctg atgtacggag agcgcgacga cccttacatc gcgctctcgc gtctgggaca
     4201 acggttggaa gcaacaatgg ctcccgaggt agttctcccg accatcgtcg agaccgtggc
     4261 gggggccctc aaagtacctt acgcggcgat agagttgaag caggacgaaa gctaccggac
     4321 cgccgcggct cacggctcgc ccgcgggcga gcctaccgtc gtcccgctcg tcaggcataa
     4381 ccaggaggta gggcgcctgg ttctctcccc acgaaccccg aacgagtcct tctccccagc
     4441 cgaccgcctc cttctccagg acctcgcccg ccaggccggg gtggcagttc acgcggtgag
     4501 gctg
//
LOCUS       DATAIR010000117         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_233969, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000117 DATAIR010000000
VERSION     DATAIR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233969"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VHM69_02955"
     CDS             complement(<1..649)
                     /locus_tag="VHM69_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739381.1"
                     /translation="MTEQPPQDEMREKQRREPRDAAYWARYAETLKVSGVAEGATNIN
                     VEGRRAVGPLQGFGKMWQKTFRVSLTGAEVTPAEVIRTWKDNYKDFWPEGNRFYAPLS
                     GIAPGEVALISGSLPGGVTLSTGVMVLYADDESFTLMTPQGHPFSGWITFSSFEDEDY
                     TVAQAQVLMRANDPLYELGLTMGGHKMEDEMWQRTLTNLAAHFGVHEQVETNVVCV"
     gene            complement(703..1152)
                     /locus_tag="VHM69_02960"
     CDS             complement(703..1152)
                     /locus_tag="VHM69_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2739382.1"
                     /translation="MKETSLDSSLHRLRGEFEGIREIYRTRRNEMPQAQVKMNVRNAG
                     EKASIIDVEGELTAFAEAVLMDAYNQASDGQVRAIILNFEGLEYMNSSGIGLLVTLLI
                     RINREKQQLLTYGLSEHYRSIFQITRLDDAIAIHDSEEEAVRAANSM"
     gene            complement(1149..1505)
                     /locus_tag="VHM69_02965"
     CDS             complement(1149..1505)
                     /locus_tag="VHM69_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2739383.1"
                     /translation="MAVRHLNVEVRREGSVAVLDLVGEINGFAEEALNAAYAEAEAKE
                     PQTILLNFEGVDYINSTGIALIVGLLARARATHRRLLACNLSEHYVEIFNITRLSDFM
                     SVFPDEESAVAEASVR"
     gene            complement(1505..1981)
                     /locus_tag="VHM69_02970"
     CDS             complement(1505..1981)
                     /locus_tag="VHM69_02970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2739384.1"
                     /translation="MTDPKPARTDAELRRTLADFSVPSEPGNERLAMETVAEAVRVLD
                     ITGENLERLKTTVAEATMNAMEHGNKYNPEKPVEIQVLVMDSDLIVRITDQGSGPPPP
                     EDGETPDLAAKLEGMQTPRGWGLFLIKSMVDELNVTGDEGHHTVELIVHLGSKGGK"
     gene            complement(1978..3147)
                     /locus_tag="VHM69_02975"
     CDS             complement(1978..3147)
                     /locus_tag="VHM69_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2739385.1"
                     /translation="MTTQEKSRQGFWQSLFGGDGREPEEKTEPAVEVPSQEAAPELEI
                     APNDPIVAYFAGASGVVEIEKLDLDSPALRSMKDADIKMVGPLVSQGELIGLLNLGPR
                     LSQQEYSADDRKLLNDLATQTAPAVQVAQLVRQQQQQAQERERIEQELRVARLIQQTL
                     LPKELPDLPGYQLAAYYQPAREVGGDFYDFLELDDGRFGLVVGDVTDKGVPAALVMAT
                     TRTMLRASAQRLDSPGEVLRRVNDVMVPDIPPNMFVTCLYAILDPETGRLHYANAGHD
                     LPYRRRATTSGAEELRATGMPLGLLPGMAYEEKEIVLERGESVLFYSDGLVEAHDPQR
                     EMFGFPRLQGLVGAHRSGGEAMVGFLLSELARFTGDDWEQEDDITLVTLERSETA"
     gene            complement(3247..4485)
                     /locus_tag="VHM69_02980"
     CDS             complement(3247..4485)
                     /locus_tag="VHM69_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739386.1"
                     /translation="MRDRFATPLAWSLWTVTVVFLAFTVAFTALYPLYRDVVSNVVNF
                     GIAVLFVLTFQTVGALIASRRPENPIGWVFCGMALALVAAVFFGNYAEYSLVVESGAL
                     PWAETAAWVGNWIWLVALSPLGFFLLLFPDGRPLSRRWRPLVWVQGAALVAWFVSSAF
                     VPGPLVNAGYESIRNPFGIEALGGILEVSGGVSGFILLGTVLASAISIVVRFRRSRGD
                     ERRQIKWVAYAGTLIILVLILQIAAETALPETDALVEVLNLGLIVSLTSVPVAAGVAI
                     LKYRLYDIDIIINRTLVYGTLTASLALVYVGSVVLLQTLFRTLTGQESQLAVVASTLA
                     IAALFNPLRRRIQTLVDRRFYRRKYDAARTLAAFNARLREETDLAALSGELVAVVKET
                     VQPARVSLWLLPREEEQRER"
     gene            complement(4482..>4742)
                     /locus_tag="VHM69_02985"
     CDS             complement(4482..>4742)
                     /locus_tag="VHM69_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739387.1"
                     /translation="LIAALFGPLRRRVQASIDRRFYRRKYDAAKTLEQFGGRLRDEVD
                     LDTLRDDLVGVVEETLQPAHVSLWLRLPDGDGGGLGAQKRNL"
BASE COUNT          829 a         1543 c         1422 g          948 t
ORIGIN      
        1 ctacgcacac tacgttcgtc tcgacctgct cgtgaacgcc gaagtgggcg gcgaggttcg
       61 tgagcgtccg ctgccacatc tcgtcctcca tcttgtggcc tcccatggtg aggccgagct
      121 cgtagagggg gtcgttggcg cgcatcagga cctgggcctg ggcgacggta tagtcttcgt
      181 cctcgaagga gctgaaggtg atccaacccg aaaaaggatg cccctggggc gtcatgaggg
      241 tgaaggactc atcgtcggcg tagagcacca tcaccccggt cgatagtgtc acgccgccgg
      301 gcagggaacc gctgatcagg gctacctccc ctggcgcgat accggagagc ggcgcgtaga
      361 agcggttgcc ttctggccag aagtccttgt agttgtcctt ccaggtcctg atgacctcgg
      421 cgggtgtaac ctcggcgccg gtcaggctga ccctgaaggt cttctgccac atcttcccga
      481 agccctgcaa tggaccgacg gccctgcggc cctcgacgtt gatgttcgtc gcgccctcgg
      541 caacgccgct gaccttgagc gtctcggcgt accttgccca gtaggcggcg tcgcggggct
      601 ctcgcctttg cttctctctc atctcgtctt gcggtggctg ctcggtcatc gtttgcctcc
      661 tttccgaagg tctccagttc cacggaaagc cggcgggcca gatcacatcg agttcgcggc
      721 cctgacggct tcctcttccg agtcgtggat tgctatggcg tcgtcaaggc gggtgatctg
      781 gaagatgctc ctgtagtgtt cgctgaggcc gtatgtcagg agctgctgct tctcgcggtt
      841 gatacggatg agcagcgtga ccagcagccc gatgccgcta ctgttcatat actcaagacc
      901 ctcgaagttc aggatgatcg cacgcacctg cccgtcgctg gcctggttgt aggcatccat
      961 tagcacagcc tcggcgaacg cggttagctc cccttccacg tctatgatgc tggccttctc
     1021 gccggcgttc ctgacgttca tctttacctg ggcctgtggc atttcgttcc tccttgtcct
     1081 gtatatctcc ctgatcccct caaactcgcc gcgcagtcta tggagcgagc tgtccagcga
     1141 tgtctctttc atctgaccga agcctcggcc acggcgctct cctcgtcggg gaagacgctc
     1201 atgaaatcag agaggcgtgt gatgttgaag atctccacgt agtgctcgct caggttgcag
     1261 gccagcagcc tgcgatgagt cgcgcgggcg cgggccagca ggccgactat cagggctatc
     1321 cccgtcgagt ttatgtagtc gacaccctcg aagttgagca gtatcgtctg tggttccttg
     1381 gcctctgcct cggcgtaggc ggcgttgagc gcctcctcgg cgaagccgtt gatctcgccg
     1441 acaaggtcga gtacggcgac gctgccttcc cggcggacct ccacgttcag gtgacgtacg
     1501 gccatcactt acctcctttc gatccgaggt gaacgatcaa ttcgactgtg tgatgtccct
     1561 cgtctcccgt gacgttcaac tcgtcaacca tgctcttgat gaggaacagt ccccatcccc
     1621 ggggcgtctg cataccttcc agcttcgccg ccaggtcagg agtttccccg tcctcgggcg
     1681 gcggcgggcc gctgccctga tccgtgatgc gcactatgag atcgctgtcc ataacgagta
     1741 cctgtatctc taccggcttc tccgggttgt acttgttgcc atgctccatg gcgttcatcg
     1801 tcgcctcggc cacggtggtc ttgagccgct ccaggttctc ccccgtgata tccaggaccc
     1861 ggaccgcctc ggcgaccgtc tccatagcca accgctcgtt ccctggttcg ctgggcacgg
     1921 agaagtccgc cagggtccgc ctgagctcgg cgtccgtccg ggcgggtttc gggtcggtca
     1981 tgccgtctcc gatctctcca gtgttacgag ggtgatgtcg tcctcctgtt cccaatcgtc
     2041 gcctgtgaag cgcgcgagct cggagaggag aaagccgacc atagcctccc cgcccgagcg
     2101 atgggccccg acgagcccct gcaagcgtgg gaatccgaac atctcccttt gcgggtcgtg
     2161 ggcctcgacc agaccgtcgc tgtagaacag cacgctctcg cctctctcaa gcactatctc
     2221 cttctcctcg taagccatgc ctggcagaag cccgagcggc atccccgtcg ccctgagctc
     2281 ctcagcgccg ctcgtggtag ccctacgcct gtagggcagg tcgtggcctg cattggcgta
     2341 atggagccgg cccgtctcgg ggtcgaggat ggcgtagagg caggttacga acatgtttgg
     2401 cggtatgtct ggaaccatca cgtcgttcac ccttctgagc acctcgccgg gcgagtcgag
     2461 acgctgggcc gaggcacgca gcatggttcg ggtcgtcgcc attaccagtg ccgccggcac
     2521 gcccttgtcg gtcacgtcgc cgacgacgag gccgaaccgg ccgtcatcga gctcgaggaa
     2581 gtcgtagaag tcgccgccca cctcacgtgc cggctggtag taggccgcga gctggtagcc
     2641 gggcaggtcc ggcagctcct tggggaggag tgtctgttgg atcaggcgcg caaccctgag
     2701 ctcctgctcg atgcgctcgc gctcctgggc ctgctgctgt tgctggcgga cgagctgggc
     2761 gacctgcacc gccggcgcgg tctgggtcgc gaggtcgttg agtagcttcc tgtcgtctgc
     2821 cgagtactcc tgctgggaga ggcgcggccc gaggttgagg agcccgatca actctccctg
     2881 gctgacgagc ggccccacca tcttgatgtc ggcgtccttc atggatctga gagccggaga
     2941 atcaaggtcg agcttctcga tctccacaac tcccgaggcg cccgcgaagt aagccactat
     3001 ggggtcgttg ggcgctatct ccagttcagg tgcagcctcc tggctaggaa cctcaaccgc
     3061 tggctctgtc ttctcctctg gctctcgccc atcgcccccg aagaggctct gccagaaacc
     3121 ctgcctgctt ttctcctgcg tcgtcatctt acctcctcga ctcgtattct agttggcgaa
     3181 cgtctcccaa acgttacgaa tagcgttacg agaatcccta tatcgtgaat atgcagcgta
     3241 attgtctcac ctctccctct gttcttcctc ccgcggaagc agccacagag acacacgggc
     3301 tggctgcacg gtctccttta caacggccac gagctcgccg ctcagggcag ccaggtcagt
     3361 ctcctcgcgc agcctggcat taaaggccgc gagcgtcctc gcggcatcgt acttgcgcct
     3421 gtagaagcga cggtccacca gcgtctggat gcgccgccgc aagggattga acaacgccgc
     3481 tatcgcaagc gtcgaggcga cgaccgcgag ttgcgattcc tgtccggtca gcgtccggaa
     3541 gagcgtctgc agcagcacga cactcccgac gtacaccagc gccagcgacg cggtcagggt
     3601 accgtagacc agggtgcggt tgatgattat gtctatgtcg tagagtcgat acttcagaat
     3661 ggcgacgccc gcggcgacgg gtacgctggt gagcgacaca atgaggccga ggttgaggac
     3721 ctcgacgagc gcgtcggtct ccggcaatgc tgtttcggcg gcgatctgca ggatgaggac
     3781 gagtattatc agagtccctg cgtaggcgac ccacttgatc tgccttcgct cgtctcccct
     3841 cgaacgacgg aacctgacga cgatggagat ggccgaggcg agcacggtcc ccagaagtat
     3901 gaagccggag acaccccctg acacctcgag gatgccaccc agggcttcga tcccaaaagg
     3961 attgcggatc gactcgtagc cggcgttgac cagaggaccg ggaacgaacg ccgacgaaac
     4021 gaaccaggca acgagcgcgg ctccctggac ccacactaga gggcgccagc gccgagacag
     4081 cggacggccg tcggggaaca ggagcaagaa gaacccgaga gggctcaacg cgaccagcca
     4141 gatccagttc cccacccacg ccgccgtctc agcccagggc agggctccgg actcgaccac
     4201 gagggaatac tcagcgtaat ttccgaagaa gacggctgcg accagggcca gggccatgcc
     4261 gcagaagacc cagccgatgg ggttctcagg gcggcgggag gcgatcaggg cccccaccgt
     4321 ctggaaggtc aggacgaaca ggaccgctat gccgaagttg acgacgttgc tgaccacgtc
     4381 tctgtacaac ggatacagcg ccgtaaacgc gacggtgaac gccaggaaca ctaccgtcac
     4441 ggtccacaat gaccacgcga gcggggttgc aaatctgtct ctcataggtt tcgcttctgc
     4501 gccccgagtc cgccaccgtc tccatcaggc agacgcaacc acagcgagac gtgcgcgggt
     4561 tgcaacgtct cctctaccac tcctaccaga tcatctctca acgtgtccag atccacctcg
     4621 tccctcaacc tgccaccgaa ttgctccagc gtcttcgccg cgtcatattt cctgcggtag
     4681 aagcggcggt ctatggaagc ctggaccctg cggcgcaaag gtccgaagag ggctgcgatc
     4741 aa
//
LOCUS       DATAIR010000118         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_99361, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000118 DATAIR010000000
VERSION     DATAIR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99361"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VHM69_02990"
     CDS             <1..702
                     /locus_tag="VHM69_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HEX2739388.1"
                     /translation="LLEEDRAARAQDKEPKKVEADPFDELVRAVETLKRGGKTRVLGQ
                     VKQQMIVQLGSFDERNLGFKKFKDFVVEAERRNLVNTVDQDLELQVYLPDEKVPDLQE
                     ADLAAEDPKETASSNRSKSNGKPAREAPETEDDFDLLEDLTPYELKTVCTSVASLEQP
                     ATFVEIFSSIRELDERGELELSREEIGDVITAMLDADYLNKVRQKPYKEAKADMTFYA
                     LNSDKKEVKSALNGG"
     gene            714..1469
                     /locus_tag="VHM69_02995"
     CDS             714..1469
                     /locus_tag="VHM69_02995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX2739389.1"
                     /translation="MGPERLSEPVRSIALQIKEELIGAGAQAVAVVGSHAVGDAGPDS
                     DLDLLAIGHPSYAWSLQRRCGLLVSVGSRPLDEYRKSFTLPQDVCAVVPGWRGAVILH
                     DPEGLAAALVREAGDWNWGPLERRCDAWVAEQVTGYAEEVHKLVAALRDGNTSTAAVQ
                     RSLLAVHLAPVLAVRRRILYGTENRLWELVSDAMGEEWRREQACALGQGDESFEETCT
                     AALRLYVLAADDVRPLLDKLQRDVVDYALAGGS"
     gene            complement(1466..1756)
                     /locus_tag="VHM69_03000"
     CDS             complement(1466..1756)
                     /locus_tag="VHM69_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739390.1"
                     /translation="MDTLLDIGVGILRLIPLILVFYIPALMGVAIWSERGEGYRLKAI
                     LWFAVGFGTIVFLHVLFSGASGFEAAAILGLSLAQILAALALATLTVYKLAD"
     gene            1857..2240
                     /locus_tag="VHM69_03005"
     CDS             1857..2240
                     /locus_tag="VHM69_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739391.1"
                     /translation="MARVGINQRVSLRKFIDDIRRWVDEGRSDEWIASALGTSPSSVQ
                     SFRSRNAIYRRGAGSTLHDPQDYSSYEGVIEPTGPGVWFDPEVGNDPRWQKRWLSTER
                     VELRLTPTRIVLLRRGGARSSTGGS"
     gene            2259..2648
                     /gene="rnpB"
                     /locus_tag="VHM69_03010"
     ncRNA           2259..2648
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHM69_03010"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          599 a          857 c          928 g          455 t
ORIGIN      
        1 ctcctggagg aagaccgcgc ggcccgcgcc caggataagg aaccgaagaa ggtggaagca
       61 gatcctttcg atgagctcgt gcgtgccgtc gagacgctga agaggggcgg caagacgcgt
      121 gttctcggtc aggtgaagca acagatgatc gtgcagctcg gatctttcga cgaacgcaac
      181 ctgggcttta agaagttcaa ggacttcgtg gtcgaagccg agcgccgcaa cctggtgaac
      241 accgtggacc aggacctcga actccaggtc tacctgcccg acgagaaggt ccccgacctc
      301 caggaggccg acctcgcagc cgaggacccg aaggagaccg cgtcctcgaa ccggagcaag
      361 agcaacggca agcccgctcg ggaggcgccg gaaacggaag acgacttcga cctcctcgaa
      421 gacctcacgc cgtacgagct caagacggtc tgtacgagcg tcgcctctct cgaacagcct
      481 gccactttcg tggagatctt ctccagcatc cgcgagctcg acgagcgcgg cgaactggag
      541 ctttcgcggg aggagatagg tgacgtcata accgccatgc tcgacgcgga ttatctgaac
      601 aaagtccgcc agaaaccata caaagaggct aaggcggaca tgaccttcta cgccctcaac
      661 tcggacaaga aagaggtcaa gagcgccctg aacggcggct gaggccacca cccttgggtc
      721 ctgagcgtct ctcagaacca gtacgctcta tcgccctgca gataaaggaa gagctcatag
      781 gggccggggc gcaggctgtc gccgtcgtcg gaagccacgc cgtcggtgac gcggggccag
      841 actcggacct ggatctcctc gccatcggcc atccatcgta cgcctggagc ctgcagcggc
      901 gctgcggcct gctcgtctcg gtgggctcgc gacctttgga tgagtacaga aagtccttta
      961 cgctaccgca ggatgtgtgc gccgtcgtgc caggctggcg aggggcggtg atcctccatg
     1021 atcctgaggg acttgccgcc gcgctcgtca gggaggccgg agactggaac tggggtccgc
     1081 tggagcggcg ctgcgacgcc tgggtggctg agcaggtcac gggctacgcc gaggaagtcc
     1141 acaagctcgt cgccgccctc agggacggga acacctcgac ggccgccgtg caacgctcgc
     1201 tgctcgcggt acatctggca cccgtcctcg ccgtccgccg ccgcatcctc tacggcacgg
     1261 agaaccgcct gtgggaactc gtctctgacg cgatggggga ggagtggcgc cgcgagcagg
     1321 cgtgcgcact ggggcagggt gacgagtctt tcgaagagac ctgtacggcg gcgctgcgtc
     1381 tgtatgtgct cgcggcagac gacgtaaggc ctctcctgga taagcttcag agggacgtcg
     1441 tcgactacgc gctggccggc ggaagctaat cagccagctt gtagacagtg agggtggcca
     1501 gggccagcgc ggcaaggatc tgtgccaggg agaggccgag gatggccgcc gcctcgaacc
     1561 ctgaggcgcc gctgaagagg acgtgcaaaa agactatcgt gccgaagccg acggcgaacc
     1621 acaggatggc cttgagcctg tatccttcgc cgcgctcgct ccatatcgcc acccccataa
     1681 gcgcggggat gtagaacacc aggatcaagg gtatcaggcg caggatacca acccctatgt
     1741 ccagcaaagt atccacgatc caagtctaca ctaacggttc cggaatactg gaaaacagtg
     1801 gaaaaatggg cgcatcatct gtaggcttag tacccagaac aattaaatag aaggcggtgg
     1861 cgcgcgtggg aataaatcag agagtttcgc tcagaaaatt catagacgac atccgccgtt
     1921 gggttgacga gggcaggagt gacgagtgga tcgccagcgc cctcgggacg agcccttcgt
     1981 cggtgcaatc gttccgctcg cgcaacgcca tctaccgtcg cggggcaggc tcgacgctgc
     2041 acgatccgca ggactactcc tcctacgaag gggtgatcga gcccacaggc ccgggcgtct
     2101 ggttcgaccc ggaagtcggg aacgacccgc gctggcagaa gcgatggctc agcacagaga
     2161 gggtcgagct gcgccttaca ccgaccagga tcgtactcct ccgccgggga ggcgccagga
     2221 gctcgacagg cggctcgtaa ggtataattt agagggtctg ggccgtccga cccgtcgctg
     2281 cgtcgcgggg cttaagccct gcgcgtagag caaagtccgg actcgtagta gcggccgctc
     2341 gggccaataa cccgagggac ggaagggcgc aagccctccg tcacgtcaag tgcaacagag
     2401 agcagaccag ccgatggccg tgtggcgaga gccacgcgaa acaggtgatg ggtgaaaggg
     2461 tggggtaaga gcccaccggc ggtatcccga gagggaccgg ccaggtaaac caccggtgcg
     2521 agcaagccaa aatgtccggg cgcgcagcgt ccggctggta ggcgcagctc ccctcagggg
     2581 agcccgggga tcccccggag atggatggcg ggatagagca gaatccggct tacgggacgg
     2641 cccggacctt tatgtaagcg attttcgcag acccgcgtac gttttgaccc cacgttttac
     2701 ccctaccggg attccaggca agctgtcctg tagcgctgca gcgtgctaat gttcgtttta
     2761 gagcttgcgc caataggcgg gccacttttc gcacatcaca actgtgaggg gctgcgcaaa
     2821 gtctctcgac tgccccttg
//
LOCUS       DATAIR010000119         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_422349, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000119 DATAIR010000000
VERSION     DATAIR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422349"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VHM69_03015"
     CDS             <1..1283
                     /locus_tag="VHM69_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HEX2739392.1"
                     /translation="AHVDKVPLGMLAAVEVAPQGHAVRQLSNPYGIATLFSLSSEETG
                     HVIPIAKALVGLRSAVVVRTPAGDVQSKQIPAGSIKFIGENQSLTVELERGADAIMDT
                     LAQVQPLSDVQGESGTNVGGMIENVRQTMADVTEKPLTEVNIHDVLAVDCLVPQQVVG
                     GIADEFSIETATGLAIMVDTDRLLMERVAETLAEKIGVRVELGGVEANTAILGALTTP
                     GIDVPVAIIDGGAGSTDGAELQKGKVVEHVHLAGAGNMVTLLIKNELGLERFDVAEQI
                     KIYPLAKVESLFHIRHEDNGVQFFEEPLDPGLFARVVLVTDDGDLIPIESKHNLDRIK
                     TVRQEAKRKVLLRNSIRVLERISPSGNIRFLDFVAIVGRFGLDFELPQMLMNELSNYG
                     IVVGVANVRGELGPTNAVATGLIINYCQRQGEAK"
     gene            1280..1636
                     /locus_tag="VHM69_03020"
     CDS             1280..1636
                     /locus_tag="VHM69_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HEX2739393.1"
                     /translation="MMDDDPDRPAILVSLAREVDERLYGWIAVGAEEEGIPCRRVPAE
                     DTDTAAIAYTAARNSRLGVGIGVASGQVAVHERHMPAERPVVATAVEEEHAKDTCRSA
                     GSNAARLVIGVPLQLE"
BASE COUNT          366 a          536 c          640 g          296 t
ORIGIN      
        1 tcgcccacgt cgacaaggtg cccctcggca tgctggcggc cgtcgaggtg gccccgcagg
       61 ggcacgcggt ccgccagctg agcaaccctt acggcatcgc cacgctgttc tccctctcgt
      121 ccgaggagac ggggcatgtg atcccgatcg ccaaggcgct ggtcgggctc cgctcggcgg
      181 tcgtggtccg cacgccggcc ggtgatgtac agagcaagca gattcccgca ggctccatca
      241 agttcattgg agagaatcag agcctcaccg tcgagctgga gcgcggggcg gacgcgatta
      301 tggatacctt ggcccaggtt cagcctctat ccgacgttca gggtgagtct ggtacaaacg
      361 taggaggcat gatcgagaac gtccgccaga cgatggccga cgtgaccgag aagccgctga
      421 ccgaggtcaa catccacgac gtgctggccg tggattgcct ggtgccgcag caggttgttg
      481 gcggcatcgc ggacgagttc tccatcgaga cggcgacggg gctggcgatc atggtagata
      541 ccgaccggct gttgatggag cgggtcgccg agacgttagc cgagaagatc ggggtgcgcg
      601 tagagttggg cggggtcgag gcgaacacgg ccatactggg ggcgttgacc acgccgggga
      661 tcgacgtgcc ggtggcgatc atcgacggtg gggcgggctc gacggatgga gccgagcttc
      721 agaaagggaa ggtcgtggag cacgtccacc tggcgggggc cgggaacatg gtgacgttgc
      781 tcatcaagaa cgagttgggc ctggagcgct tcgacgtggc ggagcagatc aagatctacc
      841 cgctcgccaa ggtcgagagc ctgttccata tccgccacga agacaacggg gtgcagttct
      901 ttgaagaacc gctcgatccg ggcctcttcg cgcgggtggt gctggtgacg gacgacggcg
      961 acctgatccc gatagagagc aagcacaacc tggatcgcat caagaccgtg cgccaggagg
     1021 cgaagcgcaa ggtgcttctc cgcaacagca tccgggtgct ggagcgcata tctccgtcag
     1081 gcaacatccg ctttctggac ttcgtggcta tcgtagggcg cttcgggctc gactttgagc
     1141 tgccgcagat gctcatgaac gagttgagca actacggcat cgtcgtcggg gtcgccaacg
     1201 tgcgcgggga gctcgggccg acaaacgccg tcgccacggg cctgatcatc aactactgcc
     1261 agcggcaagg agaggcgaaa tgatggacga cgaccccgac aggccggcga ttctcgtaag
     1321 cctggcccgc gaggtggacg agcgcctgta cgggtggatc gccgttgggg ccgaggaaga
     1381 aggcatcccc tgccgacggg tgcccgccga agataccgac accgccgcca tcgcctatac
     1441 agccgcccgg aactcccgtc tcggcgttgg tataggcgtt gcctccggcc aggtcgctgt
     1501 ccacgagcgg cacatgcccg ccgagcggcc ggtcgtcgct accgcagtag aggaggagca
     1561 cgctaaagat acgtgccggt cggcgggctc caacgcggcg cgtctggtga taggtgtgcc
     1621 cttgcagctc gaataactcg aaggggacgg gtcgagagca agattcacgt tgtcgtaaca
     1681 attgttttgc tatgaaagga gaggcaatgg cggaagcgaa gacacaggag acgatcacgc
     1741 tcgaggacgc aaagcgggtg atcgaggcgg ccgagaacaa agcccaagag atcggctgcc
     1801 cgatggacat agccgtaacc gacgccggcg gcaacctc
//
LOCUS       DATAIR010000120         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_422524, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000120 DATAIR010000000
VERSION     DATAIR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422524"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1092)
                     /locus_tag="VHM69_03025"
     CDS             complement(283..1092)
                     /locus_tag="VHM69_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739394.1"
                     /translation="MSEDTRRDVARQAANVVGAVFQLGTTAYMGARIQEVVDEGSRSL
                     VEPAAYAFTIWGPIFLLSLAYAVYQALPASRESALFREVGPFTAGSFACTGLWSIFVP
                     ARQFALAQVMLLAIFACLAVAFLRLMRSDRGVVGRADRWLVALPLGVFFGWITAANAV
                     SLTSEAVRLGLVEGGATGEVLLGTIMLLLGGILAASVVLASQAGPAHGYLAYGATVLW
                     ALAGVVANQYDASLVTTAAAAVAAVLVGRALLVAWRGGRSRREVGRNPRPV"
     gene            complement(1095..1577)
                     /locus_tag="VHM69_03030"
     CDS             complement(1095..1577)
                     /locus_tag="VHM69_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739395.1"
                     /translation="MAEVRYWLISGLSGDRSDVLTLELAGGGRVLPVFSFEEEAAEFL
                     RARGDHRGGLRINSTDVLELIVVLSYLEVQKVLLDPMHEVDDEMAAVLVGVGRERFAE
                     SLLGGPDSRSNRSGHTGALPVTFATTTRSRHSEGISSGAVWFRVESSSAEVADAAEGS
                     "
     gene            complement(1682..2323)
                     /locus_tag="VHM69_03035"
     CDS             complement(1682..2323)
                     /locus_tag="VHM69_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2739396.1"
                     /translation="MSEEHSRRPNTGRLRGDGSETKARVMDVGEAMLAEEGYRGVSLE
                     EIARRVGVSKPALYYHFPEGKEQLFVEIAHRALRQHREGLEREMASAEDGAGRLRAAA
                     RWLMGQNEVGNPTNELRDVARFVHERHRASLAEGFYGALYGPIRRAIAAAIDSGEFRR
                     NDPDFLTWAFLGLASSMLDVARTPAESPVPRPSLSSSEMADQMVKLFVEGVAA"
BASE COUNT          503 a          863 c          719 g          423 t
ORIGIN      
        1 caaccacgtg acgacgcgat cgaggacgcc ggcgtcgagg cccgtctcgt cgaaaacagg
       61 ggccctgaac gcggccatgt tgacgacgag aatcccgaag agcgcgaacc cacgcagcgc
      121 gtcgatcgtc tggatgcgct catgcatggt gatcggggcc gaagatgcac tcatttttct
      181 acctcgccct gcgctttcct ggtccatgac ggatcttgct ttgcggctcg ttgctcacgc
      241 ctgtcctcct atgatcttgg aaatgcgacc cgtccctact cctcaaacgg gccgcgggtt
      301 ccggccaacc tctcggcgcg accggccccc tcgccacgca acgaggaggg cccgtccgac
      361 gagcacggcg gcgaccgccg cggcggcggt agttaccagc gaggcgtcgt actggttggc
      421 gacaactccc gccaaagccc acaggacggt agcaccgtag gccaggtaac catgcgccgg
      481 cccggcctgg ctcgccaaca ccaccgaagc ggccagaatc cccccgagga gcagcatgat
      541 ggtgccgagc aggacctcgc ccgtcgcccc gccttccacc agcccgagac gcaccgcctc
      601 cgaggtgagg ctgactgcgt tggcggcggt gatccaaccg aagaaaaccc cgagcgggag
      661 ggcaaccagc cagcggtcgg ccctgccaac gacgccgcgg tcggaacgca tgaggcgtag
      721 aaaggcgacg gccaggcacg cgaagatggc gaggagcatc acctgcgcga gcgcgaactg
      781 ccgcgcggga acgaagatag accacaggcc ggtacaagcg aaggaccccg cggtgaaagg
      841 acccacttcc cgaaacagcg cactctcgcg actcgcgggc aacgcctggt agacggcata
      901 ggcgagcgag aggaggaaga taggacccca gatcgtgaat gcgtaagcag cgggttcgac
      961 cagcgagcgt gaaccctcgt cgactacttc ctggatccgt gcccccatgt aagctgtagt
     1021 tccgagctgg aagacagccc ccaccacgtt cgccgcctgg cgcgccacat cccttctggt
     1081 atcttcgctc atggtcaact tccttcggcc gcatcagcga cctctgccga cgaactctca
     1141 acacggaacc aaacagcgcc agagcttatg ccctcggagt gtctggatcg agtcgtggta
     1201 gcgaaggtca ccggtaacgc gcccgtgtgc cccgacctat tcgaccgcga gtctggacct
     1261 cccagtaggc tctccgcgaa acgctctctg ccaacaccga cgagcacggc ggccatttcg
     1321 tcatcgacct catgcatcgg gtccagcagg accttctgca cttccaggta tgagagcacc
     1381 acgatgagct ccagcacgtc cgtgctattg attcgcaagc cgccccggtg atcgccacgg
     1441 gctcgcagga actcggcagc ctcttcttcg aaactgaaga ccggcaacac cctcccgcca
     1501 ccagccagct ccaacgtgag aacatccgac cggtctccgc tcagccccga gatcaaccag
     1561 tacctaacct ccgccatact catctcgcat gctctccgtt ctcccgatca cgatcctggc
     1621 ctccatttcc ggatcgactt accgataagt cagttattgg gtcgaaaaaa atgcgctacc
     1681 ttcacgccgc gactccctcg acgaagagtt tcaccatctg gtcggccatc tcgctgctgc
     1741 tcaatgaggg cctgggcacg ggagactctg cgggtgttcg ggcgacgtcg agcatgctcg
     1801 aagcgaggcc gaggaaggcc caggtgagga agtcagggtc gttccggcgg aactcgccgg
     1861 agtctatggc ggcggcgatg gcgcgccgta tcggcccgta gagtgcaccg tagaaacctt
     1921 ctgccagcga tgccctgtgc cgctcgtgga cgaacctggc gacgtctcgc agctcgttgg
     1981 tcgggttacc cacctcgttc tggcccatca accaacgggc cgccgcccgc aatcgtcccg
     2041 ccccgtcctc agcagaggcc atctcacgct ccagaccctc gcgatgctgc ctcagggcgc
     2101 ggtgggcgat ctcgacgaag agctgctcct tgccctcggg aaagtgatag tacagcgcgg
     2161 gcttgctcac gcctactctc ctggcaatct cctccagcga gacaccccga tacccttctt
     2221 ccgcgagcat ggcctccccg acatccatta cccgcgcctt ggtctcagag ccatcgcccc
     2281 gcaaccgacc cgtgttgggt ctccgtgaat gctcctcgct catgagtgct ccataatatt
     2341 acttacttac tagtaagtca atgatctagg acagcgacat tatcggggcg cctagtgtac
     2401 cgtgctgtac gcggggcacg aaaagagaca acttaccatc cgtccgaaac gtggggagga
     2461 tcgagaatga atccgaagcc ggtgatcctg gacgtagacc ctggccac
//
LOCUS       DATAIR010000121         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_49449, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000121 DATAIR010000000
VERSION     DATAIR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49449"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHM69_03040"
     CDS             <1..282
                     /locus_tag="VHM69_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739397.1"
                     /translation="TRPSQEVVERAGGADLLVHDTYGSQGAAERAHAMGHSTATESGE
                     AARAAGARHLILTHLRSSRFVDPAALVAEAEGAFGGPVDAANDLDTIYF"
     gene            357..3122
                     /locus_tag="VHM69_03045"
     CDS             357..3122
                     /locus_tag="VHM69_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2739398.1"
                     /translation="MAGPHTGTITFLFTDVEGSTSLWERNAEAMSEALSRHDEILRSV
                     IEAHNGHVFKTVGDAFHAIFSAAPDALQAALQAQRALLREEWAETGPLRVRMALHTGA
                     AEERGGDYYGPSLNRVARLLSAGHGGQVLLSLATQELVRDALPEETWLRDLGERRLKD
                     LSRPERVFQITASDLPSEFSPLKTLEGRRNNLPAQPTPLVGRERELDEVLALLRSPVV
                     RLLTLTGPGGTGKTRLGLQAAAELTDEFEDGVFFVALAAVANPTLVAPTITRTLGLTE
                     SSNQPPEEILKGYLRDRQTLLVLDNLEQILASAALLDALLSSASGLKVLVTSRTPLRL
                     YGEHEFPVPPLSLPDPGSLPPFERLTQYEAIRLFVERARAVKPDFSLTQENAHAVAEI
                     CARLDGLPLAIELAAARIKLLPPQAMLPRLGNRLKLLTGGARNLPERQRTLRNAIEWS
                     YELLDEGEKTLFARLAVFAGGSTLEAIEAVCDARGDLPVDALEAVSSLLDKSLLRQEE
                     GLEAEPRFEMLETIHEFALEKLEESNEAETIRRAHAEYFLSLAERAEPKLWGPEDAAW
                     LDRLEQEHDNMRAALSWALEHEEAELALRLGGALRWFWNMGGYYDEGRSWLEALVARV
                     GWNSSEARAKVLEGLSWLADQQGDLELAEATAEEGLKLSAEAELGEVVAADFQNVLAN
                     AARQRGDYGRAAELLEESLALHRKARDTRGVAWSLGNLAIVSTDRGDYEQAKQLYEEG
                     LALSRDLGGAELLGAYLISLGYEYLLEGDPERATTLNEEAAELYRKRSRRGLLQIALD
                     NLGWAALVRGDHQEAEVLHEESLVLCRELGDKLNGSESMEGLACAAGAEGQAGRAARL
                     FGAAEALREAAGYKQATRARSLREPYLTAARSRVNETDWAKAWEEGRSMTFEDAIAYA
                     LEKGPNS"
     gene            3760..>4559
                     /locus_tag="VHM69_03050"
     CDS             3760..>4559
                     /locus_tag="VHM69_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2739399.1"
                     /translation="MSTTRKREKPVILAVDDEPTVLSAVARDLRREYGQHYRVMRADS
                     GESALELVRELKLRNVPVALFLVDQRMPGLSGVAFLENAIEVFPDAKRVLLTAYADTQ
                     AAISAINEVALNHYLMKPWDPPEELLYPVLTDLLDDWRASFAPPFEGIRIVGHQLSAQ
                     SHQIKDFLSRNLVPYQWLDVEIDEEAGDLLELSGSDGSRLPLVLFPDGSPLTQPTNAQ
                     IAERIGLRMHPGLPFYDLVIVGAGPAGLGAAVYGASEGLSTLLVEGEAP"
BASE COUNT          888 a         1344 c         1519 g          808 t
ORIGIN      
        1 acccgtccga gccaggaagt cgtcgagagg gcgggcgggg cggacttgct cgtccacgat
       61 acatacgggt cgcaaggcgc ggcagaaagg gcgcacgcca tgggacattc tacggcgacc
      121 gagtcaggtg aagcggcccg cgcggcaggt gcgcggcacc tgattctcac ccatctacgc
      181 tccagccggt tcgtcgaccc ggcagcactg gtggccgagg ccgagggtgc gttcggaggg
      241 cctgtcgacg cggcgaacga tctcgacacc atctacttct gagcgggtgc ccggggcgct
      301 ggacgtttgc cgtcccggtg ctttaattgc caatgagaga gaaggatgcg gagcggatgg
      361 cggggccaca caccggtacg atcaccttcc tgttcaccga cgtggagggc tcaacctcgc
      421 tctgggagag gaacgcggag gcgatgtcag aagccctctc ccgtcacgac gagatcttgc
      481 gtagcgtcat agaggcccat aatggccacg tcttcaaaac cgtcggagac gccttccatg
      541 ccatcttctc ggcggcccct gatgctcttc aggcggcgct ccaggctcag agggctcttc
      601 tgcgcgagga gtgggccgag acgggaccct tgcgagtgag aatggcgctg cacacggggg
      661 cggccgagga gcggggcgga gattactacg gtccctcgtt gaacagggtt gccaggctcc
      721 tctccgcggg gcacggagga caggtcttgc tttcactggc cacccaggaa ttggtgcggg
      781 acgcgttgcc cgaagagacc tggttgcggg atctgggcga gagacgactc aaggatctct
      841 ccagacccga gcgcgtcttc cagatcaccg cctcggacct tccttccgag ttctcgccgc
      901 tcaagacgct ggaaggccgg cgcaacaacc tgccggctca gccaaccccg ctggtgggac
      961 gcgaaaggga gctcgacgag gtcctggcac tgcttcgcag ccccgttgtg cgcctgctga
     1021 cccttacagg acctggcggg acgggcaaga cgcgactcgg cctgcaggcc gctgccgagc
     1081 tgacggacga gttcgaggac ggggtgttct tcgtcgcgct cgccgctgtc gcgaatccca
     1141 cgttggtggc accaaccatc acacgaactt tggggctgac cgagagtagc aaccagccgc
     1201 cggaggagat actcaagggc tatctgcgcg acaggcagac gctgctggtg cttgataacc
     1261 tcgagcagat actcgcatcg gcggcgctct tggacgcgtt gctctcgtcg gcgtccggcc
     1321 tgaaagtcct cgtcaccagc cgtactcccc tgaggctcta cggtgagcac gagttccccg
     1381 ttcctcctct ctcgctgcct gaccccgggt ccctgccgcc ctttgagcgc ctcacccagt
     1441 atgaagcgat cagactcttc gtggagcggg ccagggccgt caaaccggat ttctcattga
     1501 cgcaggagaa cgcccatgcg gtagcggaga tctgcgcgag gctcgatgga ttgcccctgg
     1561 ccatagagct cgccgcggcg aggataaagc tgcttcctcc gcaagcgatg ctgcctcgcc
     1621 tgggcaaccg cctcaagctg ctgaccggag gagcgcgaaa cctgccagag aggcaaagga
     1681 ccctcaggaa cgccatcgag tggagctacg aactgctcga cgagggagag aagacactct
     1741 ttgcgaggct cgcggtcttc gcgggtggaa gcaccctgga ggccatagag gcagtgtgcg
     1801 acgccagggg agatctcccc gtggacgcgc tcgaagcagt ctcctcactt ctggacaaga
     1861 gcttgctgcg gcaggaagaa gggctggaag ccgagccacg cttcgaaatg ctcgagacca
     1921 tccacgagtt cgctctggag aagctcgagg agagtaatga ggcagaaact ataaggcgtg
     1981 cacatgccga atacttcctg tccctggcag aacgtgccga gccgaaactg tggggacctg
     2041 aggatgcggc gtggttggac agactggaac aagagcacga caacatgagg gcggctctct
     2101 cctgggcttt ggagcatgaa gaggccgagc ttgccctgag actaggcggg gcgttgcggt
     2161 ggttctggaa catgggagga tactacgacg agggaagaag ttggctggag gcactagttg
     2221 ccagggtcgg atggaattcg tcagaggcgc gagccaaggt gctcgagggc ttgagctggc
     2281 ttgcggacca gcaaggcgac cttgaactag cggaggcgac cgccgaagag ggtctgaagc
     2341 tgagcgcaga agcagaactg ggtgaggtag tcgcggctga cttccagaac gtgttggcga
     2401 atgcagcaag gcaaaggggt gactacgggc gggcggcgga gttgctcgag gagagcctgg
     2461 cactccaccg gaaagccagg gacactagag gcgtcgcttg gtctctcggc aacctggcca
     2521 tcgtgtcgac cgaccgcggc gactatgagc aggccaagca actctacgaa gaaggtctgg
     2581 ccctgtctag agacctgggc ggtgctgagt tgctcggtgc ttacctgatc agcctgggct
     2641 acgagtacct gctcgaaggt gatcctgaaa gagccacaac gctaaatgaa gaagctgcgg
     2701 agttgtaccg gaagcggagt cgcaggggcc tactccagat tgccctcgac aacctggggt
     2761 gggctgcgtt ggtccggggc gaccaccagg aggctgaggt cttgcacgaa gagagcctcg
     2821 tcctgtgccg ggaactgggc gataagctca acggatcgga gagtatggag ggattggcat
     2881 gtgccgccgg agctgaagga caagccggga gggcagcgag gctgttcggg gccgctgagg
     2941 cattgcgaga agcagcaggc tacaagcaag caaccagagc ccgctctcta cgagagccct
     3001 accttacagc cgcccgctcc agggtaaacg agacggattg ggccaaggcg tgggaggaag
     3061 ggcgttccat gacgttcgag gatgctattg cctacgcgct ggagaagggg cctaactcct
     3121 gatccgcctg ccgacccaac ctctgagaaa gcgtcagcgc cgtaatccac atggatggac
     3181 gatccagtcg tcgacacccc tacgctggat caatcgtcat ggttagcgcc gggcggcaac
     3241 tctgcccagc atccctcctg gatccgatcg ggtgggtcgt tgatcaggtg tgcaccggta
     3301 tcgttttgag cgttcgccgt agcgaagcag ggcacgcgca ttggcaataa cacgccacct
     3361 cgcgcaactc tggccagcgc aggagagatg cttgccacca gaatcaccgc aacaagtacc
     3421 gccaccgcca ggaccttgat cattcgcctc accggctacc tccttgctct gtgcgcttcc
     3481 ggcacgcttg ccgtagccac cttagcagac cggatcacat ggcggtatat gccggatggc
     3541 gtattttcgg agtggctcga ggtgctcctc tgaaaccgtt tctcttgcgc gcggcgttcg
     3601 cgtcgttgcg gtggcttccg ctgatatagt agacacggaa gggcgtggtg ctcgcgccct
     3661 tccgtgcgag cgaggcggat gtgaagagac aatagtgacc ggtaaggtag gcgagacctc
     3721 ggcgtcgttc gagatcgcga accggtgcgc ggggctcaca tgagtactac gcgaaagaga
     3781 gagaagccgg tcatcctcgc cgttgacgac gagccgacgg tgctgagcgc cgtggcgcgt
     3841 gatctgcgcc gggagtacgg ccagcattac agggtgatga gggctgattc cggtgaatcg
     3901 gcgctcgagt tggtgaggga gttgaagctc cgcaacgtgc cggtcgccct gttcctggtg
     3961 gatcagcgga tgcctggtct gtcgggcgtg gcgtttctcg agaatgctat cgaggtcttc
     4021 cccgacgcca agcgcgtgct gctcaccgcg tacgcggata cccaggccgc cataagcgcg
     4081 atcaacgaag tagccctgaa ccattacctc atgaagccgt gggacccacc cgaagagctt
     4141 ctctatccgg tgctcacgga tctgctcgat gattggcggg cgtctttcgc cccacccttc
     4201 gaaggcatac gcatcgtagg ccaccagttg tcggcgcaat cgcaccagat caaggatttc
     4261 ctgtcccgca acctcgttcc ttatcagtgg ctggacgtcg agatcgacga ggaggccggc
     4321 gacctgcttg agctctctgg ctctgacggc tccaggttgc cactcgtact cttccccgac
     4381 ggttcgcccc tgacacagcc gacgaacgcg cagatcgcag agaggatcgg gttgaggatg
     4441 cacccggggc tgccgtttta cgacctggtc atagtcggcg ctggccccgc tggcctgggt
     4501 gccgccgtgt acggagcgtc agagggactg agcacgttgc tcgtcgaagg tgaggcgcc
//
LOCUS       DATAIR010000122         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_142012, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000122 DATAIR010000000
VERSION     DATAIR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142012"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VHM69_03055"
     CDS             complement(<1..471)
                     /locus_tag="VHM69_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739400.1"
                     /translation="MNRVGRRLSKEDKGFTLVEVLVAATMMFAMLFALYAIFDVSVRA
                     FGYGGDRLEAVGNARLAMSKMEREIRAAHPYDLTSNPQKRHLFLNQANPAQAAVPTAT
                     RISFGNEVGGDSRIGASEVIGYYLSGHDLKRSKGGSAQTLLDSVPTDGLRFTPCR"
     gene            complement(468..866)
                     /locus_tag="VHM69_03060"
     CDS             complement(468..866)
                     /locus_tag="VHM69_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2739401.1"
                     /translation="MRSPINRTAEESGYSLVEVMVAIVVLTVAIIPMVGMLDAAIRAT
                     NASGDYDEARMCAVQKLEQVKSLPYETIVDGLPGGACEPSGFGYTIATQSIGADLRDG
                     SGEDGLARITITVNWGGENSYSVAGVVSMW"
     gene            complement(850..1485)
                     /locus_tag="VHM69_03065"
     CDS             complement(850..1485)
                     /locus_tag="VHM69_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2739402.1"
                     /translation="MRTIRSKPARVSEDERGYTLPEVMTALAIMSIILAIGVIVLLAL
                     LERWRVEAAADQFAADLRLAHTRATEQLTDWRVVFMHDGTPLSRCYSADYCMVKLDAP
                     YGAGDPAPTLDDKTPPERRELPERTKIKEVTFDPDCSHGDPDAAVSPSDCGSAQTGAT
                     RTLEFNSNGTVRTLRPGQSGTVVIGSEDGIPECPVVFNAATSRIRIGEISY"
BASE COUNT          279 a          508 c          495 g          318 t
ORIGIN      
        1 cctgcagggc gtgaaccgga gaccgtccgt cgggacggaa tccaaaagcg tctgggcgct
       61 gccgcccttc gaccgcttca agtcgtggcc gctgaggtag tagccgatca cctcggaggc
      121 gccgatcctg ctgtctcccc cgacctcgtt gccgaagctg atgcgggtgg cggtaggcac
      181 cgcagcctgc gcggggttgg cctggttcaa gaacaggtgc ctcttctgtg gattcgaggt
      241 caggtcgtaa gggtgggcgg ccctgatctc acgttccatc ttgctcatgg cgagcctggc
      301 gttcccgacc gcttccagcc tgtcgccgcc ataaccgaag gccctgacgc taacgtcgaa
      361 gatcgcgtat agcgcgaaga gcatggcgaa catcatcgtc gcggcgacga gtacctccac
      421 gagcgtgaac cccttatcct ccttagacag ccgtctaccg acgcggttca ccacatggac
      481 actaccccgg ccacgctata tgagttctcg ccgccccagt tcaccgtaat ggtgatcctc
      541 gcaagaccat cttctccaga accgtcgcgg aggtccgcgc cgatggactg cgtggctatc
      601 gtgtatccga agccggaagg ctcgcaggcg ccgcctggta ggccgtccac gatggtttca
      661 tacggaaggc tcttcacctg ttccagcttc tgcacggcgc acatccgcgc ctcatcgtag
      721 tcgccgctcg cgttcgtggc ccggatcgcc gcgtcgagca tgccgaccat gggtatgatc
      781 gctacggtca ggacgactat ggctaccatc acctcaacaa gcgagtagcc ggattcttcc
      841 gcggtcctgt taataggaga tctcaccgat cctgatcctc gaggtcgccg cgttgaagac
      901 cacagggcac tcggggatac cgtcctcgga gccgattacg acggttccgc tctgccctgg
      961 cctgagggtc ctgaccgttc cgttggagtt gaactccagg gtgcgcgtgg cgccggtctg
     1021 cgcggaccca caatcgctgg gcgaaacagc cgcatccggg tccccgtgcg agcagtcagg
     1081 atcgaaggta acctctttta tcttcgtcct ttcgggcaac tcgcggcgct ccggcggagt
     1141 cttatcgtcc agcgtgggcg cgggatctcc tgcgccgtaa ggcgcgtcca gcttgaccat
     1201 gcagtagtcg gcggagtagc aacggctgag aggggtgccg tcgtgcatga acacgacccg
     1261 ccagtccgtc agttgctcgg tcgccctggt gtgggcgagc cgcaggtcgg cggcgaactg
     1321 gtcggcagcg gcctcgaccc gccagcgttc tagcagcgcg agcaggacga tgaccccgat
     1381 ggcgaggatg atgctcatga tggctaaggc agtcatgacc tcgggaaggg tatagcctcg
     1441 ctcgtcctca ctaactcggg caggtttcga ccgtatcgta cgcacgacct aattgtcggc
     1501 gctgagcttg gcgtacttta gcgggctctc acgagcccca ataaattgtt acgggcgctc
     1561 gatgaactct atacggtagc cgtcggggtc tttgacgaag
//
LOCUS       DATAIR010000123         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_326355, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000123 DATAIR010000000
VERSION     DATAIR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326355"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VHM69_03070"
     CDS             complement(<1..1008)
                     /locus_tag="VHM69_03070"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2739403.1"
                     /translation="MRVPLSWLHDYVDFDLSVDELVDLFSLRSQEIDGVYRLGVIEGE
                     VVVGEIVDFGPHPDADRLFVAKVDLGGNEVQIVAGAANPYPGARVPVVLPGSVMADGT
                     KLRKAKLRGLESYGMMMSERELGISTDHGGILLLDESYEVGRPVADYFPVGETIVDID
                     VTPNRPDLWGMIGVARELAAILKTDYRVPEPSIQTGGKPTSDFGLRVEAEDLCPRYDL
                     RRVSDLDPGRKAPLWMRRRIFAAGMRPINAVVDATNYVMLETGQPIHAFDAAKVRGGI
                     VVRRARTGEKITLLDGSTRSLDEEMLVIADEERGLVVAGVMGAEDAEVGDETEDALIE
                     VA"
     gene            complement(1021..2043)
                     /gene="pheS"
                     /locus_tag="VHM69_03075"
     CDS             complement(1021..2043)
                     /gene="pheS"
                     /locus_tag="VHM69_03075"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2739404.1"
                     /translation="MSERIEQLKSEALSAVASSDSTTALEDVRVRFLGRSAELTGIKK
                     SIGTLSPEERKEVGRSSNLASREIEAALDSRTEEVSTREREERLREEAVDVTLPGIPY
                     QQGHLHPSLRVIDDVVDFFVGLGYRVAEGPEVETDYYNFTALGIPPGHPARTMQDTFF
                     VDDGLVLRTQTSPMQIRTMLAQEPPVYVVCPGRTYRRDSDPTHTPVFHQIEGLAVDRG
                     LTLGHLKGTLAAMARHVFGENVKIRLRPSYFQFTEPSVELDVSCFICDGTDPGCKVCK
                     GAGWLEMLGAGMVDPGVLEEVGYDPEEYTGYAFGMGPDRMAMVKYGIPDLRLFFEGDL
                     RFLRQF"
     gene            complement(2059..>2755)
                     /locus_tag="VHM69_03080"
     CDS             complement(2059..>2755)
                     /locus_tag="VHM69_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2739405.1"
                     /translation="SFEMPGTPAFSPKRAAKLNMKKHRESERLFLAEGAVLIEEAPSP
                     PIRVFEEKELIRRVSALKTPAGPVGVFPFLDVSATRLLSSAQRVVLLHGVQDPGNVGT
                     AIRSAHAFGAGVALSRGSADLYNPKTVRATMGSIFHTPVAREIDSVQFLSEAGGAGFT
                     LVAATPEAGDTPRSLPAGRLVICIGAEGSGLPEEVLDACEMRVGIPSLAPSLNASVAA
                     SILLYEAYSRVLP"
BASE COUNT          503 a          905 c          860 g          487 t
ORIGIN      
        1 ggcgacctcg atcagggcgt cctccgtctc gtcccccacc tcggcatcct cggcgcccat
       61 cacgccagcg accacgagtc cccgctcctc gtcggcgatg acgagcatct cctcgtccag
      121 cgaccgcgtc gaaccgtcga gaagggtgat cttctccccc gtcctggcac gtcttacgac
      181 gataccacca cgtacttttg cggcgtcgaa agcgtggatg ggctggcccg tctcgagcat
      241 gacgtagttc gtcgcgtcga cgacggcgtt tatgggccgc atccctgcgg cgaagatacg
      301 gcgccgcatc caaagcggcg ccttgcgtcc ggggtccagg tccgacacac gccgcagatc
      361 gtagcgagga cacaggtcct ccgcctcgac ccgaagcccg aagtcgctcg tgggtttgcc
      421 gcccgtctgg atagatggtt ctggtactct gtagtcagtc ttgaggatgg cggcgagctc
      481 tcgtgcgacc ccgatcatgc cccacaggtc aggtctgttc ggggtaacgt cgatgtcgac
      541 gatagtctct ccgacgggga agtagtccgc gaccgggcgc cctacctcgt aggattcgtc
      601 gagcagcagt atccctccgt ggtcggtaga gatgccgagc tcgcgctcgc tcatcatcat
      661 cccgtaggat tcgaggccgc gcagcttcgc cttcctcagc ttcgtcccgt ccgccatcac
      721 gctgccgggc agcaccacgg gcacgcgggc gcctgggtaa gggttcgcgg cgccggcaac
      781 gatctgtacc tcgttgccgc cgaggtcgac cttcgcgacg aagaggcggt cagcgtcggg
      841 atgcggtccg aaatctacaa tctcccccac tacgacctcg ccctcaatca ctccaaggcg
      901 gtagacgccg tcgatctcct ggctgcgaag gctgaagagg tcgacgagct catcgacgga
      961 gaggtcgaaa tcaacgtaat cgtggagcca gcttaacgga acgcgcaagg tgattccctt
     1021 ctagaactga cgcaggaagc ggaggtcgcc ctcgaagaac agccgcaagt cagggatacc
     1081 gtacttgacc atcgccatcc tgtcaggacc catcccgaaa gcgtagccgg tgtactcctc
     1141 agggtcgtag ccgacctctt cgagtacgcc ggggtccacc atcccggccc cgagcatctc
     1201 cagccagcct gcgcccttgc agaccttgca accgggatcg gtgccgtcgc agataaagca
     1261 actgacgtcc agttcgacgc tcggttcggt gaactggaag taacttggcc gcagccggat
     1321 cttgacgttc tccccgaaga cgtgcctggc catcgccgcg agcgtcccct tgaggtgtcc
     1381 gagcgtgagg cctctgtcca cggcgagacc ttcgatctgg tggaacacag gcgtgtgcgt
     1441 cgggtcggag tcgcgcctgt aggtccggcc cgggcaaacg acgtagaccg gtggctcctg
     1501 cgcgagcatc gtcctgatct gcatcggcga ggtctgtgtg cgcaagacca gcccatcgtc
     1561 aacgaagaac gtatcctgca tcgtgcgcgc aggatgcccg ggcggaatac ccagcgcggt
     1621 gaagttgtag tagtcggtct cgacctccgg cccctcggcg acgcgatagc cgaggccgac
     1681 gaagaagtca acgacgtcgt ctatgaccct cagggacgga tgcaggtggc cctgctggta
     1741 cgggataccc ggaagcgtga cgtccacagc ctcctcgcgc agtcgctcct cgcgctcgcg
     1801 cgttgagacc tcttcggtcc tcgagtcgag tgcagcttcg atctcacgcg aagcgaggtt
     1861 ggacgacctg ccgacctcct tgcgctcctc gggcgagaga gtgccgatgc tcttctttat
     1921 ccccgtaagc tcagcggatc gaccgaggaa ccggaccctg acatcttcga gagcagtagt
     1981 cgagtcgctc gaggcgacgg cggagagggc ctccgacttc aattgttcga tacgctcgct
     2041 caccgtcata gtgggcgatt atggtagcac acgcgaatac gcctcgtaga gcaggatcga
     2101 agccgccacc gaagcgttca acgaaggcgc cagcgatggt atgccgaccc gcatctcgca
     2161 ggcatcgagg acctcctcag gaagcccaga gccctccgcg ccgatgcaga tcacgagcct
     2221 acctgcaggc agcgagcgtg gcgtgtcccc cgcctccgga gttgccgcga cgagagtgaa
     2281 accggcaccg ccagcctcac tcaggaactg gacggagtcg atctcccgcg ccaccggcgt
     2341 gtggaagatg gaccccatcg tagctctgac ggtcttgggg ttgtacaggt cagcggaacc
     2401 tcgggagagc gcgactccgg cgccgaaagc gtgggctgag cggatcgcgg tgcccacgtt
     2461 cccagggtcc tggaccccgt gcagcaagac gacgcgttgt gcggaggaaa gtagcctggt
     2521 ggcggatacg tccagaaaag ggaagacccc gaccggcccg gccggggtct tgagcgcaga
     2581 gacgcgacga atcagttcct tctcctcgaa gacccgtatg ggcggactag gggcttcctc
     2641 gatgagcacc gccccctcgg ccaggaatag ccgctcggac tcgcggtgct tcttcatgtt
     2701 gagcttcgcg gcccgtttcg gggagaacgc cggggtcccc ggcatctcga agcta
//
LOCUS       DATAIR010000124         5684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_7484, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000124 DATAIR010000000
VERSION     DATAIR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7484"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="lipA"
                     /locus_tag="VHM69_03085"
     CDS             <1..870
                     /gene="lipA"
                     /locus_tag="VHM69_03085"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2739406.1"
                     /translation="EWLKARIPDGEVYRDIKETMRGLNLHTVCEEARCPNIGECWNNR
                     TATFMILGNVCTRSCGFCAVLTGKPTELDLDEPHRVADAARQMGLEHAVITSVNRDEL
                     ADGGASIFAGTIRAIRDVVPGCAVEVLTPDFKGDRDAIKTVIDARPDTFNHNIETVPR
                     LYFAVRPQAKYRRSLEVLRYAKELNPDGLTKSGFMVGLGEVEEELHQTMRDLKDHEVD
                     ILTIGQYLRPSENHLPMSRYYTPKEFADLRKYGFSLGFKHVESGPLVRSSYHAHEQTA
                     DARRSPAAGVTSV"
     gene            880..1539
                     /gene="lipB"
                     /locus_tag="VHM69_03090"
     CDS             880..1539
                     /gene="lipB"
                     /locus_tag="VHM69_03090"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX2739407.1"
                     /translation="MTLERQTPSTSVDVRRLHGLTPYAEAWDLQTDLVRLRKNDEIPD
                     TFILCEHPPVYTLGRAAKDAGNLGAGEEYLRALGAEVFWSDRGGDATFHGPGQIVGYP
                     ILRLKVRDTHAYLRALEEVIIRVLMDYGLEGWHHPEYTGVWVGENKIAAIGVKFSSGW
                     ITSHGFALNVKTDLSWFDRITPCGIKEFGVTSLSRELDREVTLMEAEGKIVERFLEWF
                     S"
     gene            complement(1673..1954)
                     /locus_tag="VHM69_03095"
     CDS             complement(1673..1954)
                     /locus_tag="VHM69_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739408.1"
                     /translation="MRRFTAEPTHHPIAWLLMPAGLLLIVSAFTSAEGSSSLVFLALG
                     LGLAADGLAEVLPSRQMTAVVGLRIVSVAAFLVAFVTAILRIILRIVFS"
     gene            complement(2041..2529)
                     /locus_tag="VHM69_03100"
     CDS             complement(2041..2529)
                     /locus_tag="VHM69_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739409.1"
                     /translation="MTRDGKLALDQKGLTRDLKAGAARGIKPRTFTNLEEALAHTNAA
                     LRANRMEASDVFPSSEIDPSIQKTSFTTMGCRGRNGYEDFWWGRRSYLDDCRTHTLVD
                     ALRAGASICGAVPSIQTRLCALLGGVGSIYIARVDSRGGHDGIYINWSPALRVIYAIR
                     SQ"
     gene            complement(2843..3367)
                     /locus_tag="VHM69_03105"
     CDS             complement(2843..3367)
                     /locus_tag="VHM69_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2739410.1"
                     /translation="MAAWLERLGLGGREMTASGHSKNFLLRVVQPSLVGLMDGSVSTL
                     APLFATAFATGSTRVTFLVGLAAAVGAGISMGFSEGLSDDGSLTGRGNPFVRGAITGL
                     ATFVGGILHTMPFLIPHVDLALYVAFGVVGFELLAISFIRYRYFDMSFLMSALQVILG
                     GALVFASGILIGSA"
     gene            3863..4141
                     /locus_tag="VHM69_03110"
     CDS             3863..4141
                     /locus_tag="VHM69_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739411.1"
                     /translation="MKKRFMVLLAALAMMLAIGGPASAAAVGGDVTFKFWDASQTQAA
                     AATQAQHGDATAAAASSAGNFSSAADADASAAIDQSLTIEQRQWNGGF"
     gene            complement(4203..>5684)
                     /locus_tag="VHM69_03115"
     CDS             complement(4203..>5684)
                     /locus_tag="VHM69_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739412.1"
                     /translation="ANSTRKPVLASWMGGPAVAAGESILNGAGIPTFAYPDTAARAFT
                     NMWKYTYNLRSIYETPEPAEAEGADRERVEEIVGHVRDSGRTILTEFEAKQVLAAYGV
                     PTVQTELARSADEAVELADRFAYPVVLKLNSETITHKTDVGGVRLNLHSADEVRHAYE
                     EMESSIKEDFDGAAVQPMVSLDGYELIVGSSLDPQFGPVLLFGSGGSLVEVYRDRALA
                     LPPLTTTLALRMMERTRIFGALKGVRGRAPVDVLSLEKLLVRISQLVVEQPWIKDLDI
                     NPLLASPEHLIALDARVVLHDPQTREEVLPRTAIRPYPKQYVYHELLRDGTPVTIRPI
                     RPEDEPLMVKFHETLSEQSVYMRYFHMMNLDQRTAHERLTRICFIDYDREMALVAERT
                     DSETGEREIMGVARLSRRGGVPDEAEFSVLISDRFQRRGLGTMLLSRLLEVGHAEGLG
                     HITAEILFDNHPMQRIARKLGFRLRRDLEEMVMKAELDLFQTV"
BASE COUNT         1150 a         1716 c         1753 g         1065 t
ORIGIN      
        1 gagtggctca aggcacgcat ccccgacggc gaggtctacc gcgacatcaa ggagaccatg
       61 cgcggcctca acctccatac ggtctgcgag gaagcccgct gcccgaacat cggtgagtgc
      121 tggaacaacc gcacagccac cttcatgatc ctcggcaacg tgtgtacccg ctcctgcggc
      181 ttctgcgcgg tcctcacggg caaaccgaca gagctcgacc tcgacgaacc ccaccgcgtc
      241 gccgacgcag ccagacagat gggcctcgag cacgccgtta taaccagcgt caaccgtgac
      301 gagctggccg atgggggagc ttcgatcttc gcagggacga tacgcgcgat ccgggacgtc
      361 gtcccaggtt gcgccgtcga ggtcctcact cctgacttca agggcgaccg tgatgcgatc
      421 aaaaccgtca tagacgcccg tcccgacact ttcaaccaca atatcgagac ggtcccgcgc
      481 ctatacttcg cggtccgtcc tcaggccaag taccggcgct cccttgaggt gctgcgctac
      541 gccaaggagc tcaaccccga cggcctgacc aagagcggtt tcatggtcgg actcggcgag
      601 gtcgaagagg aactgcacca gaccatgcgc gacctcaagg accacgaagt ggacatcttg
      661 accatcggtc agtacctcag gccctccgag aaccacctgc cgatgagccg ctactacacg
      721 ccgaaagagt tcgccgatct ccgcaagtac ggcttctctc tgggcttcaa gcacgtagag
      781 agcggaccac tggtacgcag ctcctaccac gcccacgaac agaccgccga cgccaggcga
      841 agccccgcag cgggtgtcac gagcgtctag ggtacctgca tgaccctgga gaggcagaca
      901 ccgtccacat ccgtcgatgt gcggcgcctg catgggttga ccccttacgc tgaggcctgg
      961 gaccttcaga cagatctcgt acggcttcga aagaacgatg agatccccga caccttcatc
     1021 ctttgcgagc atccgccggt ctacacgctc ggtcgggccg cgaaggacgc agggaacctg
     1081 ggcgcgggag aagaatacct tcgggccctg ggggctgagg tcttctggag cgacagggga
     1141 ggggacgcga cattccacgg accggggcag atcgtaggtt atccgatcct tcgcctcaag
     1201 gtgcgcgaca cccacgccta cctccgcgcg ctggaagagg tcatcatcag ggtgcttatg
     1261 gactacgggc tcgaaggctg gcaccacccc gagtacacgg gcgtgtgggt gggggagaac
     1321 aagatcgccg ccatcggcgt gaagttttcg tcaggttgga tcacctccca cggctttgcc
     1381 ctgaacgtga agacggacct ctcctggttc gaccgaataa ccccctgcgg tatcaaggag
     1441 ttcggcgtca cgagcctgag ccgcgagctt gaccgcgagg tcactctcat ggaagcggaa
     1501 gggaagatcg tcgagcgttt tctcgaatgg ttcagttagc ttactgcctc caatggcatc
     1561 gtctttagcc tgagtcaggc gtaacaaacg aaggtgaaga atgtccgagc atccctgttg
     1621 ccgccagcca gttttagacc gaaaggttga ttcgcctgag cctgggcgcc cgttacgaga
     1681 agacgatcct caagatgatc ctcagtatgg ccgtcacaaa ggccacaagg aaagcggcga
     1741 cgcttacgat gcgtaagccc acgacggccg tcatctgtcg tgacggcaga acttcggcca
     1801 acccgtccgc tgcaagcccc aggccaagcg ccaggaacac caggctcgag gagccttccg
     1861 cagaggtaaa ggccgatacg atcagcagta agccggcggg catcaagagc caggcgatag
     1921 ggtggtgggt aggttctgcc gtaaagcgcc gcatcattcc ctcccggggt ttggtcccga
     1981 gtcgttccgc cggcgcggtg actccggaaa agtataagag ttgtcgctcg tcgggaacaa
     2041 ctactgggag cggatcgcgt agataacgcg gagcgcgggg gaccagttga tgtatatacc
     2101 gtcgtgccct ccgcgactat cgacgcgggc gatatagatt gatcccacac cgccgagtag
     2161 agcgcatagc cgcgtctgta tcgaagggac cgcgccgcaa atgctggcgc cggccctcaa
     2221 cgcatccacc agcgtgtgcg ttcggcagtc gtccaggtaa cttctccttc cccaccaaaa
     2281 gtcctcgtac ccgtttcttc cgcgacaacc catggtggtg aaactggtct tctggatgga
     2341 aggatctatc tcgctcgacg ggaacacatc cgatgcctcc atcctgttcg cccgcagggc
     2401 ggcgttcgtg tgtgccaggg cttcctcgag attggtgaag gttctgggct tgatccccct
     2461 cgcagcaccg gctttgaggt ctcgtgtgag acctttctgg tcaagcgcca acttcccgtc
     2521 tctcgtcacc cgtatgtgct cctccattag agcaacggcg cggtcaaagt cctccgctct
     2581 tgcctgccgc ccggatgatt gctctgccgt ggctgccttt gcggatgtcg tatgcgtggc
     2641 cacctgcgcc agaagaatag tcagcacaag cgacgctcct accgtaattg accgcttcat
     2701 cctgagcctc ctctcgccgg taccatagat aaggctgttt tcggacagga ccaggcgagc
     2761 ctatagttca tcgaggcaag ttgcgatcat cccctacgga gcaggctcct ggtttgtgcc
     2821 catcattctt tgagacagaa ttctaagcac ttccgatcag gatgcccgag gcgaaaacga
     2881 gcgcgccacc gaggatgacc tgcagcgcag acatgaggaa gctcatgtcg aagtagcggt
     2941 agcggatgaa ggagatggcg agtaactcga agccgacgac cccgaacgcc acgtacaggg
     3001 ccagatctac gtgcggtatg aggaaaggca tggtgtgcag tatgcctccg acgaaagtcg
     3061 cgaggcccgt gatcgcaccc cgcacaaagg gattcccgcg ccccgtgagc gacccatcat
     3121 ccgaaagacc ttccgagaaa cccatgctga tcccagcccc aaccgcagcc gctagcccga
     3181 ctagaaacgt gacccgcgta ctcccagtcg caaacgccgt cgcaaataac ggcgccagcg
     3241 tcgagacaga cccgtccatc aacccaacaa gactcggctg tacaaccctc agcaagaagt
     3301 tcttgctgtg ccccgaagcc gtcatctccc tgcccccaag ccccaaccgc tctagccaag
     3361 ccgccaaaga ctcgcctccc aaatctgcaa tgtgtaactc acctcaacat tctaatcgtc
     3421 ctcagcaata aatcaagaat aattcttgtt cctagttaat gggaaccatt tgcgattagg
     3481 agggtcaaaa ggggattgtg ggatgcgttc gtatgccata gtgggaccgg ttctcggcaa
     3541 ggtcgaggcc cggcatcaga gatggtggtc ggggcaacgg gaattggcgt tgggtggtag
     3601 agcagtgcgt cagtgtgatg gtgacctcgc ggtccctcat gcgacttcgc gagctctctc
     3661 gggggattgc tatggtacta atttgggcga aaagaacaac aggtaaggca aaaatacgca
     3721 atttggggga tacgcaaaag cgtatgatgc gtaataaata gttgtctaca gtcacacgac
     3781 cggaactgga gaagcgaggt gagggcacag gcataggtag atgtggctca gaggctgaca
     3841 aacgaagaga aaaggagaag agatgaagaa gaggtttatg gttctgctgg cggcgctggc
     3901 gatgatgctc gcgataggtg gtccggcgtc cgctgcggcg gtcggagggg acgtgacgtt
     3961 caagttctgg gatgcttcgc agacccaggc cgcggctgcc actcaggcgc agcatggcga
     4021 cgccaccgct gcggctgctt cctcggccgg caacttctct agcgccgctg atgccgacgc
     4081 gagcgctgcg atagatcaga gcctgaccat cgagcagcgc cagtggaatg gtggtttcta
     4141 aggcttagca ccggtagtca tcagccggtc cccttgcggg ggccggcttt tttgtgctct
     4201 gtctacacgg tttggaagag gtccagctcc gccttcataa ccatctcttc caagtcacgg
     4261 cggaggcgga agccgagctt cctggcgatg cgctgcatgg gatggttgtc gaagaggatc
     4321 tccgccgtga tgtgcccgag gccctcggcg tggccgacct cgaggagccg gctaaggagc
     4381 atggtgccga gtcctctgcg ctggaagcgg tcgctgatca ggaccgagaa ctcggcctcg
     4441 tctgggacgc cgccgcggcg gctcaggcgg gctacgccca tgatctcacg ctcacccgtc
     4501 tcggagtccg tccgctcggc gaccagcgcc atctcgcggt cgtagtcaat gaagcagata
     4561 cgggtcagac gctcgtgggc cgtgcgctgg tcgaggttca tcatgtggaa gtagcgcatg
     4621 tagacgctct gttctgagag ggtttcgtgg aacttcacca tcaaaggctc gtcctcgggt
     4681 cggatcggac gtatcgtcac gggtgtgccg tcgcgcagca attcgtggta gacgtactgc
     4741 ttggggtaag gacggatggc ggtgcgcggg aggacttcct ccctcgtctg ggggtcgtgc
     4801 aaaacgacgc gggcgtcgag ggctatcagg tgctcgggcg aggcgaggag cgggttgatg
     4861 tcgagatctt tgatccaggg ctgctcgacg acgagctggc ttatgcgtac gagcagcttc
     4921 tctaaggata aaacgtcgac cggagccctg cccctgacgc ccttgagcgc cccgaagatg
     4981 cgggtccgct ccatcatccg aagggcgagc gtcgtcgtga gcggagggag cgcgagggcg
     5041 cggtccctgt acacctcgac gagagagccg cctgagccga agagcaggac gggaccgaac
     5101 tgcgggtcca ggctgctgcc gacgatcagc tcgtaaccgt cgaggctgac cataggctgc
     5161 acggcggcgc cgtcgaagtc ctccttgata gaggactcca tctcctcgta agcgtgcctg
     5221 acctcgtccg cgctgtgcag gttcaggcgc acgccgccga cgtcggtctt gtgggtgatt
     5281 gtctcggagt tgagcttgag taccacgggg taggcgaacc tatcggctag ttccaccgct
     5341 tcgtccgcgg agcgggccaa ctcggtctgt acggtcggga cgccgtaggc ggcgaggacc
     5401 tgcttcgcct cgaactcggt gaggatggtg cgtccggagt cgcggacatg gcccacgatc
     5461 tcctctactc gctccctgtc ggcgccttcg gcttctgcgg gctcgggtgt ttcgtagatg
     5521 ctgcgcaggt tgtacgtgta cttccacatg ttcgtgaaag cgcgggccgc cgtgtcggga
     5581 taggcaaagg tagggatgcc tgcgccgttc aggatcgact ctcccgcagc gaccgcaggc
     5641 ccgcccatcc aactcgcgag cacgggcttg cgggtgctgt tcgc
//
LOCUS       DATAIR010000125         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_188020, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000125 DATAIR010000000
VERSION     DATAIR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188020"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VHM69_03120"
     CDS             <1..486
                     /locus_tag="VHM69_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2739413.1"
                     /translation="AALIEAGVAQPGRIGVTGTSYGGYSAWWAITHFEPHVAAAPICG
                     MTDLVVDYYATRPDLRPYSEEMMGGSPEEVPDQYQERSPINFVSNIQGDLLIVQGMKD
                     PNVTPDNVYAVTKALQRENIPYELLTFDDEGHGISRPENLRVLYPRLADFFHTAFDES
                     P"
     gene            719..1237
                     /locus_tag="VHM69_03125"
     CDS             719..1237
                     /locus_tag="VHM69_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739414.1"
                     /translation="MGQTAQTMVRTSQESARIFTDYTVKAQELNTQLAQRSVETWIDG
                     LRKQTELSQDMAQALFGKAEEQAEVYRSFFEKWGHLPVAGFPFAGNNVTFPFQKQGMR
                     LVDTATKNAEETLATATFPIAGYDEKNVEEISKQLDFLTDEQIRQLKAYERKNKNRQS
                     LIERFDSKLKAS"
     gene            1456..2820
                     /locus_tag="VHM69_03130"
     CDS             1456..2820
                     /locus_tag="VHM69_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2739415.1"
                     /translation="MDVMRGLGESGDSTPLRGVENLMLHFTPYAEDWSKLPVIVSGEG
                     CYVTDDKGNTYIDGLAGLFTTQVGHGRSELAEVAARQMRELGFFPNWNMHHPRSLELA
                     ARISEIAPGDLSSTFFVSSGSEAVETVIKLARQYHKTNGEAERYKVISRKIAYHGTTM
                     GALSVTGLPTFKAPFEPLLQGFHHVTNTQQDPEGAADAIEEAIEFGPPETVAAVILEP
                     VQNAGGCLVPPPDYWTRVREICDRHGVLLVSDAVICAFGRLGEWFGIERFDVVPDMTS
                     FAKGVTSGYLPMGGVVVNEKISSTLRSKAPMFMHGSTFGGHPVSSAVAIENINIIERE
                     GLLGNVHDLEGHFGDELRRMAADHPIVKEVRGMGFFWAVEINPERADGTPLEGDEYQK
                     YFKGVVSRKLLEGGLICRFDDKEDPVIQFSPALVADKEIISKIAGITDDALTELEREL
                     GYRS"
BASE COUNT          612 a          844 c          888 g          510 t
ORIGIN      
        1 gcagcgctca tcgaggctgg cgtggcgcag ccggggcgca tcggtgtcac gggcacctcc
       61 tacggagggt actcggcgtg gtgggcgatc acacacttcg agccgcacgt cgccgccgcc
      121 ccgatctgcg gtatgaccga cctcgttgtt gactattacg ccacacgccc cgacctcagg
      181 ccgtacagcg aggagatgat gggaggctcc ccggaagagg tcccagacca ataccaggaa
      241 cgctccccta tcaacttcgt aagcaacatc cagggagacc tcctgatcgt ccagggcatg
      301 aaggacccga acgtcacccc ggataacgtc tacgccgtca cgaaagccct gcagcgcgag
      361 aatatcccct acgaactcct caccttcgat gacgagggcc acggcatctc ccgccccgag
      421 aacctgcgcg tcctctaccc tcgcctcgca gacttcttcc acacagcctt cgacgaatct
      481 ccttgacgag aagccgagcg ccagcgctga gaatcgtcca gcgtgcgatc cgcggagacc
      541 ggggcaggtc ttgttgtatg agtatcaatc gtgcccgaaa cagagagatt cagcccaaaa
      601 aatctcggct ttgatgttta gtcctatatg tgcagggtac atataggtgc aggagggtaa
      661 gagacagcta cgaaatggag gcactacagt gacgcacgct aagagagact ttgagaagat
      721 gggtcagact gcgcagacga tggtcaggac ctcgcaggag tccgccagga tcttcacgga
      781 ctacactgtg aaggcgcagg agctcaacac gcagctcgcc cagcgctccg tcgagacctg
      841 gatagacggg ttgcggaagc agaccgagct ttcccaggac atggcgcagg ctctcttcgg
      901 caaggctgag gagcaggccg aggtgtaccg ctcgttcttc gagaagtggg gccatctacc
      961 ggtagcgggc ttcccgttcg cgggcaacaa cgtaacgttc cccttccaga agcaggggat
     1021 gcggctcgtt gacaccgcca ccaagaacgc ggaggagact ctggcgacgg caacgttccc
     1081 gatcgccggc tacgacgaga agaatgtcga ggagatctcc aagcagctcg acttcctcac
     1141 cgacgagcag atccgccagc tcaaggccta cgagcggaag aacaagaacc gccagagcct
     1201 gatcgagcgc ttcgacagca agctcaaggc ttcgtaacag agacagcacg aacgtaatac
     1261 agagggccgg gtcttagatc ccggcccttt ttcctgctct ctgaaatctt cgacctgcgc
     1321 tactcggatt gcaccggata caatgacact tcaggagagc cctacacagg tgccggatct
     1381 tgaccatatt acagtaactg tgtagtattc gtgcaaaccg aggggccgtc cggttccgcc
     1441 ttgaggaggt attacatgga cgtgatgagg ggtcttgggg agagcgggga cagcacgccg
     1501 ctccgggggg tcgagaatct gatgctccac ttcacgccat acgccgagga ctggtcgaag
     1561 ctgccggtca tcgtctctgg cgaggggtgc tatgtcacgg acgacaaggg caacacttac
     1621 atagacggac ttgcggggct cttcacgacg caagtcgggc acgggaggag cgagctggct
     1681 gaggtcgcgg ccaggcagat gagggagctc gggttcttcc ccaactggaa catgcaccat
     1741 ccgcgttcgc tggagcttgc ggccaggatc tccgagatag caccggggga tctctcctcc
     1801 accttcttcg tgtcttcggg ttccgaggcc gtcgagacgg tcatcaagct ggcgcgccag
     1861 tatcacaaga cgaacggcga ggccgagcgt tacaaggtta tttcgcgcaa gatcgcctac
     1921 cacggcacga cgatgggcgc gctctcagtg acggggttgc ccacgttcaa ggctcccttc
     1981 gagccgttgc tgcagggctt tcatcacgta accaatacgc agcaggaccc cgagggggcg
     2041 gctgacgcca tcgaggaggc catagagttc gggccgcccg agacggtggc ggcggtcatc
     2101 ctcgagcctg tgcagaacgc gggcgggtgc ctcgttccgc cccctgacta ctggacgcgg
     2161 gtgcgtgaga tctgcgaccg tcacggcgtc ctgcttgttt cggacgcggt gatctgtgct
     2221 tttggacgcc tcggcgagtg gttcggtatc gagcgcttcg acgtagtccc cgacatgacc
     2281 agctttgcca aaggtgtgac atcgggctat ttgccgatgg gcggggtcgt tgttaacgag
     2341 aagataagca gcaccttgag gagcaaagcc ccgatgttca tgcacggctc gaccttcggg
     2401 gggcatcctg tctccagcgc ggtcgccatc gagaacataa acatcatcga gcgcgagggg
     2461 ctgctaggga acgtccacga cctcgaaggg cacttcggcg acgagttgag gcggatggcc
     2521 gctgaccatc ctatcgtcaa agaggtcagg gggatgggat tcttctgggc tgtggagatc
     2581 aaccccgagc gggcagacgg gacgccgctc gagggcgacg agtaccagaa gtacttcaag
     2641 ggcgtcgtct ccaggaagct tttggagggc gggctcatct gccgcttcga cgacaaggaa
     2701 gaccctgtca tccagttctc cccggcgctc gtagcagaca aggagatcat ctcgaagata
     2761 gcaggtatca cggacgatgc cctcaccgaa ctcgaacgag agctcggcta ccggagttag
     2821 ggcttgaccc gcgccgtcag cgcaagtagg acag
//
LOCUS       DATAIR010000126        13639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_7568, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000126 DATAIR010000000
VERSION     DATAIR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7568"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VHM69_03135"
     CDS             complement(<1..247)
                     /locus_tag="VHM69_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739416.1"
                     /translation="MAIRKLEVCAHLLGDKDLQRWCTFHLGGYVYRLPRAPSELSSDC
                     AEKVISKLVELKVPFDTEEIFPRFGTSGGGFESIEFIE"
     gene            complement(420..1199)
                     /gene="istB"
                     /locus_tag="VHM69_03140"
     CDS             complement(420..1199)
                     /gene="istB"
                     /locus_tag="VHM69_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2739417.1"
                     /translation="MTTKRRGMTEEAADAAIDQACRMLRMPTIRNSFTDYADRAGREQ
                     MSYRGFLAELLLAECDDRARRRSERRIKAAKFPREKSLRAFDFDANPNIDPAVIHTLA
                     KCEWVQKGQPLCLIGDSGTGKSHLLIALGTEAAMAGYRVKYTLATQLANELVEAADEM
                     TLAKTIARCGRVDLLCIDELGYMQLDRRGAELLFQVLTEREEKASVAIASNESFGGWT
                     KTFTDPRLCAAIVDRLTFGGTILETGTDSYRLAQATKQRTT"
     gene            complement(1196..2872)
                     /gene="istA"
                     /locus_tag="VHM69_03145"
     CDS             complement(1196..2872)
                     /gene="istA"
                     /locus_tag="VHM69_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041767552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX2739418.1"
                     /translation="MTQPRSRVDLYAAIRRDARSGMSNRALQRKHGVGFRTVTAALES
                     AWPKERKQPPKRGSRLDAYRVVIDDWLRSDLDAPRKQRHTAKRIFDRLLDEHDGAGVV
                     SYWMVREYVATRRREIRVEVGQEPANAFIPQEHLPGREAEVDFGDVAIRLRGELVTCA
                     LFSLRLSYSGKAVHRVSASAGQEAFFEGHVHAFNVLGGVPTGKIRYDNLKAAVASVIG
                     FSRQRVEADRWTAVRSHYGIEAFYCQPGIQGAHEKGGVEGQIGWFRRNHLVPIPEVDS
                     LDELNAMVDAWDEADDARRIGSRARTVGEHFATEQPLLAPLPTEPFETGRWFTPRVDR
                     YAQVTVRMNKYSVPARMVGRQARVLLNASDLVVFDGRTEIARHERLMTKGSTRVDLDH
                     YLEVLLRKPGALPGATALEQARASGRFTSVHDAWWAAACKAHGDADGTRALIEVLMLH
                     RHLPHDHVVAGLATALRAGALTADAVALEARKYNDTADGGADDTTADHQSLMSGDRAR
                     GEGEIPAVRSLTERRLRAHIPADTRPLPSVEKYDQLLANRRDTPNEGAAP"
     gene            complement(2978..3211)
                     /locus_tag="VHM69_03150"
     CDS             complement(2978..3211)
                     /locus_tag="VHM69_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739419.1"
                     /translation="MSRFDVVGGITGLIQGVLVTNVFVTTQGRAVVLTWASVGLDLAY
                     SLLIGSVVGRTPHSGPTQYRPPKPCAAHDQAPR"
     gene            complement(3297..4130)
                     /locus_tag="VHM69_03155"
     CDS             complement(3297..4130)
                     /locus_tag="VHM69_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739420.1"
                     /translation="MNEMAFRVTSNDGTTIAYYRKGSGPAVVLVGGAQDDGAENAPLI
                     PALAEHFTVYNYARRGRGASGFTEPYAVEREIEDLDALIAEAGGSAHLFGASSGGALA
                     LEAAAAGSAIDTIAVWEVPYAVGDDIRPRFEEYVADTRRAFDAGRDEEVLELFMRMTG
                     ASDDNIAARKAAGKQTAHWADSVALAPTLVHDSLFLNRYQLPADRLATVTQPVLVTTG
                     GPITVPYMAGLPSDFFDRAADELADLLPHAQRETLEGPDHVVDPQIVGPLLLRFFSSE
                     H"
     gene            complement(4158..4580)
                     /locus_tag="VHM69_03160"
     CDS             complement(4158..4580)
                     /locus_tag="VHM69_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2739421.1"
                     /translation="MSTILKNNSETVAHTPGRASSIGVWILQVVLAAIIAGGGISKLA
                     GDPVMVDMFADIGAGQWLRYLVGALEVAGGVGLLIPALAGLASLGLAALLTGAVITDQ
                     FVLEQSPWLPLALLVVAAVIARARWSRTEAVVGTLLRR"
     gene            complement(4673..5245)
                     /locus_tag="VHM69_03165"
     CDS             complement(4673..5245)
                     /locus_tag="VHM69_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2739422.1"
                     /translation="MSATKLLVLGIVHLSGGAHGYQVRSELQSWRAEIWAKIKPGSIY
                     HALKKAAADGLLTEHAEPGNSGPERVLYRVTARGRDEIVELVRDGLRRTHDPTMLNAS
                     IAMLPMLTRTDAIAHVNERVARLEAELVEQAKWREHPNPDIPEHVRDQAELWAGHART
                     ELEWAKSLSKRLAEGAYTMADDPGSWRTVP"
     gene            5605..6573
                     /locus_tag="VHM69_03170"
     CDS             5605..6573
                     /locus_tag="VHM69_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739423.1"
                     /translation="MTDLPEGYETRAPAREDVEAVTAMISACQLADTGETDISVEEML
                     DDWHAVDLAEDAVMVTAPDGRIAGYADVLNRSFVMVSVYGYVHPDFREKGIGSYLVAW
                     GERWTRDRMPQAPENARVVVQHYINAANEEARRLMESSGYAPIRGVYVMETRLDDAPP
                     LPHWPTGISVRTFVPDRDERTTYEAVEEAFRDLWGRPTSPFERFLRETEKETFDPSLW
                     FIAEDGDEIAGVILCKTLAGEGWVEVVGVLRPWRKRGLGLALLRHAFAEFHTRGIHKV
                     SLSVDAESITGAPRLYGRAGMSVRESYLIHLKELRPGVDLGIRYEE"
     gene            6708..7355
                     /gene="lexA"
                     /locus_tag="VHM69_03175"
     CDS             6708..7355
                     /gene="lexA"
                     /locus_tag="VHM69_03175"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX2739424.1"
                     /translation="MREALHPRRVEILRYLARRARGEGIPPSVREIGLEVGLRSSQTV
                     HHHLGRLEEEGYVERVGRGARTLRLTGKGWEAAGHMALLGRIAAGRGLEAVAYGDDAY
                     SLAAELLSARSGRRRYLLRVVGQSMVGARIEDGDLLVVEEDEAPADGAVVVALLGGGD
                     EVTVKRLYREGENVRLRPENGEHQEILVPADEIQVQGRVVYVVHPPRRGPSGDGS"
     gene            7362..7889
                     /locus_tag="VHM69_03180"
     CDS             7362..7889
                     /locus_tag="VHM69_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739425.1"
                     /translation="MGYVLDLLWGMLSYVAFTGAGGLVGYYFARKRTEHEVGYGRRVE
                     TVERIQDSVAEVVEELVAAREYVFGSGPFDGGPAKRIGRILDDFETYCVRREIWLNRE
                     TFSRLEDLTVTLRAYQRELASLPLYYEDPDFEREHQRVGRELDLWLETGLPAAREELA
                     DAFRGMLGVGRWRRG"
     gene            8068..9933
                     /locus_tag="VHM69_03185"
     CDS             8068..9933
                     /locus_tag="VHM69_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX2739426.1"
                     /translation="MRDYLLTLRSGLGRTLRHLRLAVAAYGVLLISLLLTALAWYYVR
                     QTVDAQNQVRFDETVRATRAAVDRRTDAYLDALFGARGVFLASKAVEREEWESYVGGI
                     ETESRLEGLQALGYTEYVEPDERVAYEEEARKRGMPELRPDLDPGGERSAYFPLSLVA
                     PWDEANLTMINQDVYTDPVHRAAMDRARDTGSPQATKMVYVLTEPTPGSTADLALRPG
                     FAVYLPVYAQREPIETADERRRALRGFVVGYFRRDGLLDDVFGSGFDPAIDFEVYDGR
                     DVESSSLLYDEDGVERAAEEAYDPLFSEKSRIGVAGRVWSLYFATLPGFKKGTESNLP
                     AFVLASGIAVSLLLFGISWMLVRSRILAERASEELGDANRELEGTNRELEAFSYSVSH
                     DLRAPLRTIDGFSQILQEDYEDVLDEEGMDYLGRVRAASRHMATLIDDLLDLSRVGRR
                     PLRREPVDLSQLAAGIIEDFRASQPEREVEFVYGEDIVAWGDVSLLKVALENLLGNAW
                     KFSAREPEARIEFGADRKPGPGFLAPVYYVRDNGAGFDPAYSDKLFGAFQRLHTQDEF
                     EGTGIGLATVARIVHRHGGRVWAEGRVGEGATFCFTLGGRDLGNRAVPARKAELA"
     gene            9926..>13639
                     /locus_tag="VHM69_03190"
     CDS             9926..>13639
                     /locus_tag="VHM69_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2739427.1"
                     /translation="MRETRIKGSEAPVRESLRVLLVEDSENDALLLVRELRRGGYQPL
                     CERVYTPEDMERALGEAETRGEPFQVVISDYYMPRFGAPEALELLRELGHDVPFIVVS
                     GKIGEDAAVGVMKAGADDYLTKENMSRLCPAIERELREAEVRREREKAERALDRSEDR
                     FRRLVEQAADAIFVHDLDGRIVDVNRQACDSLGYSREELLSMSVQDFEANFVPGSFRR
                     LWEQITSGPPRTLDGIHRRKDGTTFPVEVRVGVFEAEEHPLMLALVRDVSERREAEKK
                     IRETEARYRTLVEQIPAVTYVQEPIESDNPKAVTYMSPQYEAMLGYPPEKEILDEEHW
                     LKVLHPDDRERVLAEELRTDETGEPFRIEYRQIARGGRVVWVRDEATLVRDEDGNPLY
                     WLGVQYDITEQKRTEEDLRSSEERFRVTFEQAAVGIVQVGLDGEWLRYNDKLCDILGY
                     TPEELETVSVFDLISPEDVEKDYDRGVSMLAGEISNYSEEKLIVGKDGRRVWINLTVS
                     LVHDGSDDPRYFIAVLEDIGKRKQAEKDFRLRDRAIAASSNGIVITDPNLPDNPIVYV
                     NPAFERITGYPVDEVLEQNCRFLQGEGDRDQPALDELREAIKEGRYSSVILRNRRKDG
                     TPFWNELSVSPVRDEEGKLTHFVGVQNDVTERQRTQEELRQSEERFKVLAQEVVEGIL
                     LIENGRIIDANRSVTEMFGYELEELIGKEAIELTLPEIRELVRRRIADEDTTVYESEG
                     LKKDGTAFPIEIRPRHLPYSGRRIRVTSVIDLTERKRAEDAVRESEKRFRALVQNASD
                     IILVLDAEGIIRYESPAVERVLGYSPEERVGINAFERIHPDDRARVLQVFSENLSRPG
                     PFPPVEYRVRHKDGAWRHLEAIGENLLHDPVIRGVVVNSRDVTERKRAEQTLKKSERR
                     FRHLFENSSDALFIHDERGRFVDCNAEACRALGYTREELLSLSVADVATRLISEEERR
                     EGETLWERALRGEPGRIVGLDDNEMRRKDGTTFPVEVGIGSIEYGERRMIFASARDIT
                     GRKRAEEALRQSEARLQAILDNTTAVVYIKDEEGRYSLINQRFKELFHVTRDEVLGKT
                     DHDIFPEEVADEFRTNDEEVLRSGVPLEVEEVVPQDDGIHTYVSVKVPLNGPDGTPYA
                     ICGISTDITERKRAEEALLMSEERYRAVVEQSAEGIYLLDAATKRLIESNPALQMMLG
                     YSASELKGMHIYDIAAHSQDDLDENVRVTLSKRA"
BASE COUNT         2480 a         4136 c         4653 g         2370 t
ORIGIN      
        1 cctcgataaa ctcaatgctc tcaaatcctc caccggaggt tccaaacctg gggaatatct
       61 cctcggtatc aaacgggact ttgagttcta ctagcttaga gataaccttt tctgcgcagt
      121 cgctacttag ctcggacgga gccctcggga gtcgataaac atatccacca agatggaagg
      181 tacaccatcg ttggagatct ttatcgccta gtaagtgagc acaaacctca agctttctta
      241 tcgccacgga aactgctctc ggctttgcgc tttgcagaga ctccaaggct tctgcgctaa
      301 gttcttggct gcggaggttt cgtccgacac cgcttgttgg cgagttctga caccgccggg
      361 cacgtgtcgg tgccgcggat gttggtttgc gtcgacaccg cttccgcgcc cgacgggtgt
      421 caggttgtcc gctgctttgt ggcttgggcc agacggtagg agtcggtgcc ggtctcgagg
      481 atggtgccgc cgaaggtgag tcggtcgacg atggctgcgc atagccgggg gtcggtgaag
      541 gtcttggtcc agccgccgaa ggactcattg gacgcgatcg cgaccgaggc cttctcctca
      601 cgctcggtga ggacctggaa gaggagttcc gcgccgcggc ggtcgagttg catgtagccg
      661 agttcgtcga tgcacagcag gtcgacgcgg ccgcagcggg cgatggtctt cgccagcgtc
      721 atctcgtcgg cggcctcgac gagctcgttg gcaagctggg tcgccagggt gtacttgacc
      781 cggtagccgg ccatggcggc ctcggtgccg agtgcgatca gcagatggga cttgccggtg
      841 ccggagtccc cgatcagaca cagcggctgt cccttctgga cccattcgca cttggcgagg
      901 gtgtggatca cggcgggatc gatgttgggg ttggcgtcga agtcgaaggc ccgcagggac
      961 ttctcgcggg ggaacttggc ggccttgatc cggcgttcgg accggcgtcg ggcgcgatcg
     1021 tcgcactcgg caagcaatag ttcggcgagg aacccgcggt aagacatctg ctcacgccct
     1081 gcccggtcgg cgtagtcggt gaaggagttg cggatggtgg gcattcgcag catccggcac
     1141 gcttggtcga tcgcggcgtc ggctgcttct tcggtcattc cacggcgctt ggtggtcacg
     1201 gtgcggctcc ttcgttcggg gtgtcacgtc ggttggccaa cagctggtcg tacttctcca
     1261 cgctcggcag cggccgagtg tcggccggga tgtgggcgcg gagccggcgc tcggtcagcg
     1321 atcgcacggc agggatctcg ccttctccgc gtgccctgtc gccgctcatc agggactggt
     1381 ggtccgctgt cgtgtcgtcg gctccgccat ctgctgtgtc gttgtacttg cgcgcttcca
     1441 acgcgacagc gtccgcggtg agcgcgcctg cacgcagcgc cgtcgcgagt ccggcgacga
     1501 cgtggtcgtg tggaaggtgc cggtgcagca tcaggacctc gatgagggca cgggtgccgt
     1561 cggcgtcacc gtgggccttg caggccgcgg cccaccaggc gtcgtgaacg gacgtgaacc
     1621 gcccggatgc cctggcctgc tccagcgctg tcgcaccggg tagcgcgccc ggtttgcgga
     1681 ggaggacctc gagatagtgg tccaggtcga cccgggtcga gcccttggtc atgagccgtt
     1741 cgtggcgggc gatctcggtc ctgccgtcga acacgaccag gtcgctggcg ttcaggagca
     1801 cccgggcttg acggccgacc atgcgcgcgg gcacggagta cttgttcatc cggaccgtga
     1861 cctgggcgta cctgtccacg cgcggggtga accagcggcc ggtctcgaac ggctcggtcg
     1921 gaaggggcgc gaggagtggc tgctcggtgg cgaagtgttc cccgaccgtg cgggcgcggg
     1981 acccgatccg ccgggcgtcg tcggcttcgt cccaggcgtc gaccatagcg ttgagctcgt
     2041 ctagggagtc gacttctggg atggggacga ggtggttgcg tcggaaccag ccgatctgcc
     2101 cctcgacgcc gcctttctcg tgcgcacctt ggattccggg ctggcaatag aaggcttcga
     2161 tgccgtagtg ggaacggacg gcggtccacc tgtcggcctc gacccgctgg cgggagaacc
     2221 cgatcacgct cgcgacggcg gccttgaggt tgtcgtagcg gatcttcccg gtcggcaccc
     2281 cgccgagcac gttgaaggcg tggacgtgac cctcgaagaa ggcttcctgc ccagccgagg
     2341 cactgaccct gtgcacggcc ttgcccgagt aggagagccg aaggctgaac agcgcgcagg
     2401 ttacgagctc gccgcgcagg cggatggcga cgtcgccgaa gtcaacctcg gcctcgcggc
     2461 ccgggaggtg ctcttgcggg atgaacgcgt tggcaggttc ctgtccgacc tcgacgcgaa
     2521 tctcacggcg tcgggtggcg acgtactcgc ggaccatcca gtacgacacc accccggccc
     2581 cgtcatgttc gtcaagcagt cgatcgaaga tccgcttcgc cgtgtgtcgc tgcttccgcg
     2641 gcgcgtcgag gtccgagcgc agccagtcgt cgatcaccac tcggtacgcg tcaagccgtg
     2701 agccgcgttt gggcggttgc tttctctcct tcggccatgc agattccagc gccgctgtca
     2761 cggttcggaa gccgacgccg tgcttgcgct gcagcgcgcg gttggacatc ccagatcggg
     2821 catcccgtcg gatcgccgcg tacaggtcga cccgggaacg cggctgcgtc atcggtggca
     2881 ctcctgacgt gagcacgccg tgggcacgac tttcggcacc gggccaccgc cccggggaac
     2941 ggtgaccgca tgcgaccaca gcccggaatc actggcccta ccgaggtgcc tggtcatgtg
     3001 ctgcgcaggg cttcggtggg cggtactgag tcggcccgga gtgcggggta cgaccgacga
     3061 cggagccgat gagcagcgag taggccaggt cgagtcccac tgaagcccag gtcagtacga
     3121 cggcccggcc ttgcgtggtg acgaagacgt tggtgacgag cacgccctgg atcaggcctg
     3181 tgatgccgcc gacgacatca aacctcgaca ccacctgtcc gccccgtgtc cctggcgcga
     3241 cgaagatctg cttgcgcggt gcctaatcgg tcttcccgca acacctcgcg ggcccctcag
     3301 tgttcgctgc tgaagaaccg tagcaacagc gggccgacga tctgtgggtc gacgacgtga
     3361 tccggcccct cgagggtctc ccgttgagcg tggggtagga ggtctgcaag ctcgtcggct
     3421 gcgcggtcga agaagtccga gggcaggcct gccatgtagg ggaccgtgat cggtcctccg
     3481 gtggtgacca ggaccggttg ggtgaccgtt gccagtcgat cggccggcaa ctggtagcgg
     3541 ttgaggaaga ggctgtcgtg gaccagcgtg ggcgcgaggg cgaccgaatc cgcccaatgc
     3601 gccgtctgct tgcctgccgc tttcctggcc gcgatgttgt cgtcggaggc gccggtcatg
     3661 cgcatgaaca gttcgagaac ctcctcgtct cgcccggcgt cgaaggcgcg tcgggtgtcc
     3721 gcgacgtact cctcgaatcg cgggcgtatg tcgtccccga ccgcgtacgg cacctcccac
     3781 acggcgatcg tgtcgatggc tgacccggcc gcggcggctt ccagcgccag agcgccacca
     3841 gaggacgccc cgaacaggtg tgccgagccg cccgcctccg cgatcaacgc atccaggtcc
     3901 tcgatctccc tttccacggc gtagggctcg gtgaagccgc tcgcgccgcg tccccgacgg
     3961 gcatagttgt aaaccgtgaa gtgctcggcg agagcaggta tcaagggagc gttctcggct
     4021 ccgtcgtcct gtgcgccgcc taccaggacg acagccgggc cgcttccctt ccggtagtag
     4081 gcgatggtgg tgccatcgtt tgatgtcacg cgaaaggcca tctcgttcat ctcacatctc
     4141 cgttcgagag gtttgtctca gcgcctgagc agcgtgccga cgacggcctc ggtgcgcgac
     4201 caccgcgccc ttgcgatcac ggccgccacg acgagaaggg cgagcggcag ccaagggctc
     4261 tgctcgagca cgaactgatc ggtgatgaca gcaccggtca gcaatgccgc caaccccaga
     4321 ctggccaggc ccgccagagc cgggatcaga agtccaaccc ctcccgcgac ctccagcgct
     4381 ccgaccaggt accggagcca ctggccagcc ccgatgtcgg cgaacatgtc caccatgacc
     4441 gggtcgccgg cgagcttcga gattccgccg ccggcgatga tcgccgccag cacgacttgc
     4501 agaatccaga ccccgatgct gctcgcccgc ccgggcgtgt gcgcaacggt ttccgagttg
     4561 ttcttgagga tggtgctcat tcggctctcc ttgccgtgtc cgggcctggg cgactagtca
     4621 agtttggtta ctcggccaaa gtacacgagg aggagcgtct agtcaaggtt gattagggga
     4681 ccgttcgcca cgaacccgga tcatcggcca tggtgtaggc gccctcagcc agtcgtttgc
     4741 tcaggctctt cgcccactcc agttcggtgc gtgcatgtcc cgcccacagt tcggcctgat
     4801 cgcggacgtg ttcgggaatg tcggggttcg ggtgctcccg ccacttggcc tgctctacga
     4861 gctccgcttc caggcgcgcg acccgctcgt tgacgtgcgc gatggcatct gtcctggtca
     4921 gcatgggcaa catggcgatc gacgcgttga gcatggtggg gtcatgggtg cgccgcagac
     4981 cgtcgcggac cagttcgact atctcgtcac gtccccgagc tgtcacgcgg tacagaacgc
     5041 gctccggccc ggaattgccc ggctcggcgt gttcggtgag gaggccatca gccgccgcct
     5101 tcttcagcgc gtgataaatc gagccgggct tgatcttggc ccaaatctcc gcacgccagc
     5161 tctgcagctc ggaccgcacc tggtagccat gcgcgccgcc ggagaggtgc acgataccga
     5221 gtaccaggag cttcgtcgcc gacatgcgcc cacctccgct caatctgctc ggactcgctc
     5281 cgcttgcgcg ccgccagggt ggtgtcaact cccaccaaca ctagggtgcc ctcctggtcc
     5341 gggtggtgtc agcactgacc tacaaacggt gctggtggag acctccaaag ccaaagttct
     5401 cgacaccttg catgcacact tgccatttta ctactcctgt tagtgcttcc gtcccgtaac
     5461 cattcgagag cacgagtcga ttgcatttta cctctgctcc tgtctattac atccaagtac
     5521 agacgctatg gtgcgtagag attgtgagta cgtcagtaca gaatagtaag gactaaggga
     5581 tcttgacgtg ctaagtttgt cgccatgact gacttgcctg aaggttatga gacgagagcg
     5641 ccagctcgcg aagacgtgga ggccgtgacg gccatgatct ccgcctgcca gctggccgat
     5701 accggcgaga cggacattag cgttgaggag atgctcgacg actggcacgc ggtggacctc
     5761 gcggaggacg ccgtgatggt gaccgcgccc gacggacgca tcgccggcta cgccgacgtg
     5821 ctgaaccgct ctttcgttat ggtctccgtc tacggctacg tccaccccga cttcagggag
     5881 aagggcatcg gttcctacct cgtcgcctgg ggcgagcgct ggacgcgcga ccgtatgccg
     5941 caagcccccg agaacgcgcg cgtcgtcgtg caacactaca taaacgcggc caacgaggaa
     6001 gcgcgacgcc tgatggaaag ctcgggctac gcgccgattc gaggtgtgta cgtgatggag
     6061 accaggctgg atgacgcccc gccactaccg cactggccca cgggcatctc cgtccgcacc
     6121 ttcgtccccg acagggacga gcgtacgacc tacgaggccg tcgaagaagc gttcagagat
     6181 ctgtgggggc gtcctaccag tccattcgag cgttttctcc gggagacgga gaaggagacc
     6241 ttcgaccctt ccttgtggtt catcgcagag gacggagacg agatagcagg cgtcatcctt
     6301 tgcaagacgc tcgccggcga gggatgggtg gaagtcgtcg gagtactgcg cccgtggcgg
     6361 aagcgcgggc ttgggctcgc cctacttcgc cacgccttcg ccgagttcca cacgcgcggt
     6421 atccacaagg tcagcctcag cgtggacgcc gagagcatca ccggcgcccc aaggctctac
     6481 ggacgggccg gtatgagcgt cagagagagc tacctcatac atttgaagga gcttcgcccg
     6541 ggcgtggatc tcggcatcag gtacgaagag tagacacgcc cgcaagagta tcgaccgccg
     6601 catctcgatc tgctgaggat gcgctgatgg cgttcgcaat gaggtcgaac cgagaatatc
     6661 aaacaaatgt ttgacgagga tgctcacttg cgttagggtg tgcattgatg agagaggcat
     6721 tacatccgag gcgggtggag atcctgcgct acctggcgcg tcgggcgagg ggtgagggga
     6781 ttccgccgag cgtgcgtgag atcggtctcg aggtgggctt gaggagctct cagaccgtcc
     6841 accaccacct gggcaggctt gaggaggaag gatacgtgga gagggtgggg aggggtgcga
     6901 ggacgctcag gctgacgggg aagggttggg aggcggcggg gcacatggca ttgcttggca
     6961 ggatcgccgc cggtcgtggc cttgaggcgg tcgcgtacgg tgacgacgcc tattcgctgg
     7021 cggccgagtt gctctcggcg cgttcgggga gacggcgata cctgctcagg gttgtggggc
     7081 agagcatggt aggggcgagg atagaggatg gggacctcct ggtagtggag gaggacgagg
     7141 cgccggcgga cggcgctgtg gtcgtcgcgt tgctcggggg tggggatgag gtgacggtca
     7201 aacggctcta ccgcgaggga gagaatgttc ggctcaggcc ggagaacggg gagcaccagg
     7261 agatcctggt ccctgccgat gagatacagg tacaggggag ggtggtctac gtcgtccatc
     7321 ctccgcgtcg aggcccttcc ggggacgggt cttgaattcg gatggggtat gttctggacc
     7381 tgctgtgggg tatgctctcg tacgtggcct tcacgggcgc gggaggtctc gtcgggtact
     7441 acttcgctcg taaacgcacc gagcacgagg tgggctacgg tcgcagggtc gagaccgtcg
     7501 agcgcatcca ggactcggtg gccgaggtag tcgaggagct ggtggcggca cgcgagtacg
     7561 tcttcgggag cggccccttc gacggggggc cggccaagag gatcggtcgg atcctagacg
     7621 acttcgagac gtattgcgta aggcgtgaga tctggctgaa ccgcgagacg ttctcccggt
     7681 tggaggacct caccgtcacc ctgcgcgcct accagcgaga gctcgcctca ctgcctctct
     7741 actacgagga ccctgacttc gagcgggagc accagcgcgt tggccgtgaa ctcgacctgt
     7801 ggctcgagac cgggcttccc gccgcccgcg aagagctcgc cgacgctttc agagggatgc
     7861 tcggggtcgg gcgctggcgg cggggatgaa taaatcttgc gggtcatctg tgcgacgggt
     7921 aacattgctc aatacggtgc gtcacgggcg gattgtccgg catcattcgt ccaagggaaa
     7981 ggttcgtgaa gcggtgagcg caacgttatc ggcgacggat gcgttcgtcg ccgcgttgcc
     8041 cggggctggg ggtctatcat aggcgggttg agagattacc tgctaactct gcgttccggg
     8101 ctcggccgga ccttgcgcca cctgcgcctc gcggtggctg cttacggggt gctcctgatc
     8161 tcgctcctgc tcacggcgct cgcctggtat tacgtgcgac agacggtcga tgcgcagaac
     8221 caggtccgct tcgacgagac ggttcgggcg acgagggctg ccgtggatcg gcggacggac
     8281 gcttacctcg acgcgctgtt cggcgcccgc ggcgtattcc tcgcgagcaa ggcggtcgag
     8341 cgtgaggagt gggagagtta cgtggggggc atagagacgg agagccggct cgaggggctg
     8401 caggctctcg gctacaccga gtatgtggag cccgatgaga gggtggctta cgaagaggag
     8461 gccaggaaac gcggaatgcc ggaacttcgg cctgacctcg accccggcgg ggagcgttcc
     8521 gcctacttcc cgctctcgct ggtcgcgcct tgggacgagg ccaatctgac catgatcaac
     8581 caggacgtct acaccgatcc cgtccacagg gccgccatgg acagggcgcg cgataccggt
     8641 tcgccgcagg ccacgaagat ggtctatgtg ctgacggagc caacgcccgg ctcgaccgcc
     8701 gacctcgccc tcaggcccgg tttcgccgtg tacctccccg tctacgccca gcgggagccc
     8761 atcgagaccg ccgacgagag gcggcgcgct ctcagggggt tcgtcgtcgg atatttcagg
     8821 agggacgggc tgctcgacga cgtgttcggg agcggcttcg acccggccat agacttcgag
     8881 gtctacgacg gcagggacgt tgagtcgagc tcgctcctct acgacgaaga cggcgtggag
     8941 cgtgccgcag aggaagcgta cgacccgctc ttctccgaga agagccgcat cggtgtggcg
     9001 gggcgcgtgt ggagcctgta cttcgccacc ttgcccggat tcaagaaggg cacggagagc
     9061 aacctgccgg ccttcgttct cgcgagcggc atagcggtca gcctcctgct gttcgggatc
     9121 tcctggatgc tcgtgcgcag ccgcatcctg gccgagcgcg cgagcgagga gctcggtgac
     9181 gccaacaggg agctcgaagg gacgaacagg gagctcgagg cgttctctta ttcggtctcc
     9241 cacgacctgc gggccccctt gaggaccata gacgggttct cccagatact gcaggaggac
     9301 tacgaggacg tgctcgacga agaggggatg gactacctcg ggcgcgtaag ggcggcgagc
     9361 aggcacatgg ccacgctgat agacgacctt ctcgacctct ccagggtcgg acgcaggcca
     9421 ctgcgcagag aacccgtcga cctgagccaa cttgccgcgg ggataatcga ggatttcagg
     9481 gcgtcgcagc cggagcggga ggtcgagttc gtatacggtg aggacatcgt ggcctggggc
     9541 gacgtgagcc tgctcaaggt ggcgctggag aacctgctcg gcaatgcgtg gaaattttcg
     9601 gccagggagc cggaggcacg catcgagttc ggcgcagaca ggaaacccgg cccaggtttc
     9661 ctcgcccccg tctactatgt gcgagacaat ggggccggct tcgatccggc gtactcggac
     9721 aagttgttcg gggcattcca gaggttacac actcaagatg agttcgaggg cacgggaata
     9781 gggttggcga cggtcgcgcg catcgttcac aggcacggcg gcagagtctg ggccgaggga
     9841 agggtcggcg agggggcaac cttctgcttc acgctcggcg ggcgggacct cgggaaccgc
     9901 gcggtcccgg cgaggaaggc cgaacttgcg tgagacccgc atcaagggat cagaggcgcc
     9961 cgtgagagag agcctgcgcg tgctgctcgt cgaggactca gagaacgacg cgttgctcct
    10021 tgtgcgggag ctcaggcgcg gcggatacca gcctctctgc gagagggtgt atacgccgga
    10081 agacatggag agggcgctcg gggaagcgga gaccaggggc gagcctttcc aggtggtgat
    10141 ctccgactac tacatgcccc gcttcggggc tcccgaggcc cttgagctgc tgcgggagct
    10201 cggccacgac gtacctttca tagtcgtctc gggcaagata ggagaggacg cggcggtcgg
    10261 ggtcatgaag gcgggggcag acgactacct caccaaggag aacatgagca ggctgtgccc
    10321 cgccatcgag cgcgagctca gggaggcgga ggtaaggagg gagcgcgaga aggcggagag
    10381 ggcgctggac cggagcgagg accgcttcag gcgcctcgtc gagcaggccg cggacgccat
    10441 cttcgtgcac gacctcgatg gcaggatagt cgacgtaaac cgccaggcgt gcgacagcct
    10501 cgggtactcc agagaggagc tcctctcgat gtcagtccag gatttcgagg cgaacttcgt
    10561 ccctggatct tttcgcagac tgtgggagca gatcacctct ggcccgccgc gcacgctcga
    10621 cgggatacac cgccgcaagg acggcaccac cttccccgtc gaagttcgcg tcggcgtgtt
    10681 cgaggcggag gaacacccgc tcatgctcgc cctggtccgc gacgtctccg agcgcaggga
    10741 agcggagaag aagatcaggg agacggaggc ccgttacagg acgctcgtgg agcaaatacc
    10801 ggccgtcacc tacgtgcagg agcccatcga gtcggacaac ccgaaggccg tcacgtacat
    10861 gagtccgcag tacgaggcca tgctcggtta tccaccggag aaggagatac tcgacgagga
    10921 gcactggctc aaggtgctgc atcccgacga ccgggagcgc gtgctggccg aggagctcag
    10981 gaccgacgag accggcgaac ctttcagaat cgagtaccgg cagatcgccc gtggcgggag
    11041 ggtggtgtgg gtgcgcgacg aggctacgct cgtcagggac gaggacggca atcctctgta
    11101 ctggctcggc gtccagtacg acatcacaga gcagaagcgg actgaagagg acctcaggag
    11161 tagcgaagag cgcttcaggg tcaccttcga gcaggccgcc gtcgggatcg tgcaggtcgg
    11221 gctggacggc gaatggctga ggtacaacga caagttgtgc gacatacttg gttacacacc
    11281 cgaggagctc gagaccgtga gcgtcttcga cctcatctcc cccgaggacg tcgagaagga
    11341 ctacgatcgg ggcgtcagca tgctggccgg ggagatcagc aactactccg aggagaagct
    11401 catcgtcggc aaggacggca ggcgcgtctg gatcaacctg accgtctccc tggtgcacga
    11461 cggctcggac gatccccgct acttcatcgc cgtgctcgag gacatcggaa aacgcaagca
    11521 ggccgagaag gacttccgcc tccgtgacag ggccatcgcc gcaagctcca acggcatcgt
    11581 gataactgac cccaatctcc ccgacaaccc catcgtctac gtcaaccctg ccttcgagcg
    11641 gataacgggc tatcctgtgg acgaggtcct ggaacagaac tgccgcttcc tgcagggtga
    11701 aggagaccgc gaccagcctg cgctggacga gttgcgcgaa gcgatcaaag aggggcgcta
    11761 cagcagcgtc atcctcagga accgcagaaa agacggcacg ccgttctgga acgagctctc
    11821 cgtctcgccc gtgcgcgacg aggaaggaaa gctcacccac ttcgtcgggg tacagaacga
    11881 cgtgaccgag cgccagagga cacaggagga gctcaggcag agcgaagagc gcttcaaggt
    11941 cctcgcacag gaggtcgtag agggcatcct cctgatagag aacggcagaa tcatcgacgc
    12001 caaccgaagc gtgaccgaga tgttcgggta cgagctagaa gaactgatag gaaaagaggc
    12061 catagagctc acgctgcccg agatcaggga attggtgagg cggcgcatcg ccgacgagga
    12121 caccacggta tacgagtcag agggactcaa gaaagacggc accgccttcc ccatagagat
    12181 aaggcccagg cacctgccgt actcggggcg caggatcagg gtgacctcgg tgatcgacct
    12241 gacggagcgc aagcgggctg aggacgccgt gcgggagagc gagaagcgct tccgcgccct
    12301 ggttcagaac gcctcggaca tcatactggt gctcgacgcc gagggcatca tccgctatga
    12361 gagccccgcc gtcgagcgcg tgctcggcta cagtcccgaa gagagggttg ggatcaacgc
    12421 gttcgagcgg atccaccccg acgaccgcgc gagggtcctg caggtgttct ccgagaacct
    12481 ctccagaccc ggtcctttcc ctcccgtgga atacagggtg cgccacaagg atggcgcctg
    12541 gcgccacctc gaggccatcg gcgagaacct gttgcacgac cccgtcataa ggggtgtcgt
    12601 cgtcaacagc cgcgacgtta ccgagcgcaa gagagcggaa cagacgctca agaagagtga
    12661 acgccggttc aggcacctct tcgagaactc ctccgacgcc ctctttatcc acgacgagcg
    12721 cggccgtttc gtggactgca acgccgaggc ttgcagggct ctgggttaca cccgcgagga
    12781 gctgctctcg ctctcggtcg ccgacgtggc cacgcgattg atctcggagg aggagcgcag
    12841 ggagggagag acgctctggg agcgggcgct gcgaggagag cccgggagga tagtagggct
    12901 ggacgataac gagatgaggc gtaaggacgg cacgacgttc cccgtggagg tggggatagg
    12961 ctccatcgag tacggagagc ggcgcatgat cttcgcctcg gcccgcgaca ttaccgggcg
    13021 aaagcgggcc gaggaggccc tcaggcagag cgaggcgcgt ctgcaggcca tcctggacaa
    13081 caccacggcg gtcgtatata tcaaggacga agagggacgg tactcgctga tcaaccagag
    13141 gttcaaggaa ctcttccatg tgaccaggga tgaggtcctt ggaaagacgg atcacgacat
    13201 cttccctgag gaggtcgctg acgagttcag gaccaacgac gaggaagtac tccgttccgg
    13261 agtcccgctg gaggtggagg aggtggtccc ccaggacgac gggatacaca cctacgtctc
    13321 tgtcaaggtg cccctgaatg gccctgacgg gaccccctac gccatctgcg gcatctcgac
    13381 cgacataacg gagcgcaagc gggcggaaga ggcgctcctt atgagcgagg aacgctacag
    13441 ggctgtcgtc gagcagtccg cagagggtat ctacctcctc gacgctgcta ccaagcgtct
    13501 catcgagtcc aacccagctc tgcagatgat gctcggctac tccgcttcag agctcaaggg
    13561 tatgcacatc tacgacatag cagcccactc ccaagatgac ctcgacgaga acgtaagagt
    13621 aacgctctct aagagggca
//
LOCUS       DATAIR010000127         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_422746, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000127 DATAIR010000000
VERSION     DATAIR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422746"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="trxB"
                     /locus_tag="VHM69_03195"
     CDS             complement(<1..958)
                     /gene="trxB"
                     /locus_tag="VHM69_03195"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2739428.1"
                     /translation="MTQRIYDVVIIGSGPAGYTAALYAARANLKTLVFQGFESGGQLM
                     LTSDVENYPGYRDGVMGPEMMDDFEAQAARFGAEMRPENIERVDFSERPFKLWAEDED
                     EPTLAKAVVIATGAKAKWLGLESEQQLMGKGVSGCATCDAFFYRGKKVAVFGGGDTAM
                     EEAGVLAKFADEVVLIHRRDEFRASKIMLQRARKNPRITFITDTVIEEILGEDSVEGV
                     HVKNTKTGEEQTIEVSGFFAAIGHSPATDLFKGQVEMDEGGYILQKENTMTSVPGVFA
                     AGDVSDTRYRQAVTAAGDGCRAAIDSERWLEEQGEAEGAEDPA"
     gene            complement(1080..2345)
                     /locus_tag="VHM69_03200"
     CDS             complement(1080..2345)
                     /locus_tag="VHM69_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2739429.1"
                     /translation="MHSESVVRDPTLAPEGHRKIDWAAEHSPVLNIVRDKYLKDSTFD
                     GLGVGVALPIEAKTAYLTVVLAEAGADVAVASPGPFFVQDDVAAALAERGVTVYASSE
                     NDPEDADRELERVLDRVTDGKEAVLIDDRAGLVRLAHTTRRNLLGYIRGASEETTSGV
                     AKFRAMAREGILEFPVIAANDARCKYRFDNRYGTGQSTLTAIMQNTNLMVGGKRVVVL
                     GYDWCGKGIARYAAGLGARVSVCEVDPVLGLEAYADGFDVLPALDAAEIGEVFITATG
                     SKDVLTAAHFERMRDGAILANAGGVDVEIDVDGLTATATGAREVRRNVEEFTMGDGRR
                     LHLVGRGMVVNLSAGDGHPVEIMDLTFAIQALCAYHLANEHASMENKVHLLPKEIDDE
                     VARTKLDAVGMGVDALTPGQEEFLAGWRE"
BASE COUNT          377 a          830 c          698 g          486 t
ORIGIN      
        1 cggctgggtc ctcggcgcct tccgcctcgc cctgctcctc gagccagcgc tcgctgtcga
       61 tggcggcccg gcagccgtcg ccagccgccg ttacggcctg gcggtagcgc gtatcggaga
      121 cgtcgccggc cgcgaacacg cccgggacgc tcgtcatggt gttctctttc tggaggatgt
      181 agccaccctc gtccatctcg acctgtccct tgaagaggtc agtcgcggga gagtggccga
      241 tggcggcgaa gaagccgctc acctcgatgg tctgctcctc accagtcttg gtgtttttta
      301 catgcactcc ctcgacggag tcctcaccca gtatctcctc gatcaccgtg tccgttatga
      361 acgtgatcct ggggttcttg cgagccctct ggagcatgat cttggacgcc ctgaactcgt
      421 cgcggcggtg gattaagacg acctcgtcgg cgaacttggc gaggactccg gcctcttcca
      481 tcgcagtatc tcccccgccg aagacagcga ctttcttgcc cctgtagaag aaggcgtcgc
      541 aggttgcgca gccgctgacg cccttgccca tcaactgctg ctcgctctcg agcccgagcc
      601 acttggcctt ggcgcccgtg gcgatgacga cggccttcgc cagggtcggc tcgtcctcat
      661 cctcggccca caacttgaac ggacgctccg agaagtccac cctctcgatg ttctcggggc
      721 gcatctcggc cccgaagcgc gcggcctggg cctcgaagtc gtccatcatc tcagggccca
      781 tcacgccgtc cctgtacccc ggatagttct cgacgtccga ggtcagcatt agctgcccac
      841 cgctctcgaa accctggaag accagcgtct tcaggttcgc ccgcgcagcg tacagggcag
      901 ccgtgtaacc cgccggaccc gagccgatga tcacgacgtc gtatattctc tgggtcatct
      961 aaaacactcc tcttatatgc aaagtctcta atctccctat tttaatacat ctaacaaacc
     1021 gtatagttca acctttcaca gtgggtgtcg ttgtatcaca cggtcgggcg cgaacccatt
     1081 cactcccgcc agccggccag gaactcctcc tgcccgggcg tcagggcgtc gacgcccatg
     1141 ccgaccgcgt cgagcttcgt gcgggcgacc tcgtcgtcta tctccttggg aagaaggtga
     1201 actttgttct ccatcgaggc atgttcattc gcgaggtggt aggcgcagag ggcctgtatc
     1261 gcgaaggtga ggtccatgat ctcgaccggg tgcccgtctc cggccgagag gttgacgacc
     1321 ataccgcgcc cgacgaggtg cagccgcctg ccgtcgccca tggtgaactc ctcgacgttg
     1381 cgccgtacct cgcgggctcc ggtcgccgtg gccgtcaggc cgtcaacgtc tatctcaacg
     1441 tccacgccgc ccgcgttggc caggatcgcc ccgtcgcgca tccgctcgaa gtgcgcggcc
     1501 gtcaacacgt ccttgctgcc cgtcgccgtg atgaagacct cccctatctc cgccgcatcg
     1561 agggccggca gcacgtcgaa cccgtccgcg taggcttcga gaccgagcac agggtccacc
     1621 tcgcagaccg agacgcgggc gcctagccca gcagcatagc gcgcgatgcc cttgccgcac
     1681 cagtcgtagc cgagcaccac gacgcgcttg ccgccgacca tcaggttggt gttctgcatg
     1741 atcgccgtaa gggtggactg gcccgtcccg tagcggttgt cgaaacggta cttgcacctg
     1801 gcatcgttcg ccgcgatgac cgggaactcg agtatcccct cccgggccat cgccctgaac
     1861 ttcgcaaccc cgctagtcgt ctcctccgac gcgccgcgga tgtacccgag caggtttcgt
     1921 cgcgtcgtgt gcgcgagcct cacaagcccg gccctgtcgt ctatcaagac cgcttccttg
     1981 ccgtctgtca cccggtcaag gacacgttcg agttccctgt cggcatcttc agggtcgttc
     2041 tcggaggagg cgtagaccgt cacgccgcgt tccgcgaggg ctgcggccac gtcgtcctgt
     2101 acgaagaatg gtcccgggct cgcgacggcg acatcggccc ccgcctccgc gagcacgacc
     2161 gtcaggtagg ccgtcttcgc ctcaataggg agggcgacgc cgacgccgag tccatcgaag
     2221 gtgctgtctt tcaggtattt gtcgcggacg atgttcagga cgggcgagtg ttcggcggcc
     2281 cagtctatct tcctgtggcc ttcaggggcg agggttgggt cccttacgac ggattcgctg
     2341 tgcataacgt taccttaagt tcctgtctct tcggcggcca tctcctcatg g
//
LOCUS       DATAIR010000128         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_100115, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000128 DATAIR010000000
VERSION     DATAIR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100115"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHM69_03205"
     CDS             <1..289
                     /locus_tag="VHM69_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739430.1"
                     /translation="LDVQTTRTTGGPEIQVGAAQARERSVRPDRHVMDLDELITYYSG
                     VFRTTVALKGRFGGWRRSGKKAFREVDAYQQMVRRYIQALDETVKLTRQET"
     gene            349..1329
                     /locus_tag="VHM69_03210"
     CDS             349..1329
                     /locus_tag="VHM69_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2739431.1"
                     /translation="MGPFLEEGRLRALWRLAIQYAAYMVLVPLLFNLLVVTWLLAGSG
                     GQAVSGGLDTSAFLASPALPLVSAVAGLVGAALTVWLAGRFLDRRPFSEFGFHLGVGW
                     WLDFLFGMVLGALLITIVFLVELGLGWVRITGAFEAYGTGAPFAISMLLPVAAFLCVG
                     FSEETVFRGYQLKNAAEGLNYPAIGPRGAILVAWVSSSVFFGVLHADNPSATPISTFN
                     IVLAGLMLGFGYVLSGELAIPIGLHITWNLFQGAVYGFPVSGFGPFGATLLATKQVGP
                     DLWTGGSFGPEGGLLAPAVMLLGFCLIALWTYLRTGDFSLHSRIAEGPHR"
     gene            1485..2810
                     /locus_tag="VHM69_03215"
     CDS             1485..2810
                     /locus_tag="VHM69_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739432.1"
                     /translation="MEREVERLAGGKMGGETRIGTVATASFIGTAIEAYDLYIYGFAA
                     AFVLGPLFFPGFSETAQTLAAFATFAASFLSRPLGAIIFGHFGDRLGRKAMLVLSLLV
                     MGFATFCIGVLPGFDTLGVAAPLSLVVLRFLQGVGFGGEWGGAVLMSAEHAPRGGRGF
                     YASFPQLGPPVGFLFASGAFLLVTTAFSDAGFQSFGWRIPFLISIVLVGVGLYVRVRV
                     AETPVFRLAMEEQSAPGVPVIEVVRRYPKELLLTTGTMVVLFGTFYVFSTFAPSYETT
                     ELGLQTSTTLYITMSAIIFMGLTIPVSAAVSDRVGRRNLCLAAAALFGLWAFPMFWLI
                     DTGNPILIILAFAVLMVIYGMMYGPVAAFFSEQFETRVRYSGISLGYNLGGIIGGAFT
                     PIIATELLTSTGASWSISLYLVALAAISFFSVFLLSETHSKDLSKIQSR"
     gene            complement(3041..4453)
                     /locus_tag="VHM69_03220"
     CDS             complement(3041..4453)
                     /locus_tag="VHM69_03220"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEX2739433.1"
                     /translation="MMGWLSTLMERRPLRSWAIVAIVGALAMAAVLLAGMQTAQASHP
                     EVSLAGSDFEIDTDANLKVDDAAPSIDWASVPQGSSANGFEVRKAEEFTGAQDNSFGQ
                     GTKEDTAVPTVVNGSIPPNKSDLLTFGVTLEENANGRFLHMYWHRVQEPSGTTNMDFE
                     FNHATATSSNGVTPVREEGDILIQYDLAQGGTNPQLFLSEWVTTGDKSQCQAANSTPC
                     WSDRINLSAADLDGDGVNDATGSINTSAIPASESDGLGAISPRTFGEATVDFDTVAGD
                     DPCVGFGSAYLKSRSSDSFTAALKDFIAPEPLNIDRCGAIQVTKTAKHADQSGATSPN
                     LEATFTVTQGSTTFGTITTDPDTGIGCLGDLPLGSYTVTETVAASGYALDTNNPKTAN
                     VTSTGSCSSGFTNVSFENTPLTDITVSVDSQVPGGTASTISCDDPDSTSGSTGAGGDG
                     SITADDLAPGTYTCTVVVDP"
BASE COUNT          760 a         1543 c         1444 g          994 t
ORIGIN      
        1 gctcgacgtt caaacgactc gcaccacggg cggaccggag atccaggtag gagcggcgca
       61 ggcgcgcgag cgtagcgtcc gccccgaccg ccacgtgatg gacctggatg agctcatcac
      121 ctactacagc ggcgtcttcc gcaccaccgt tgccctcaaa gggaggttcg ggggttggag
      181 gcgctccggg aagaaggcgt tcagggaggt ggacgcttac cagcagatgg tccgccgcta
      241 cattcaggca ctggatgaga cagtcaaact aacgcgccag gagacctgaa gcccgcgccc
      301 agagagaaca tctgctgacc gtagaggcgt gttaaggacg gaggctgctt gggaccattt
      361 cttgaggaag gcaggctcag ggcgctgtgg cggttggcga tccagtacgc ggcctacatg
      421 gtgctggtcc cgcttctctt caacctgctc gtcgtcacgt ggttgctggc gggttcaggt
      481 ggacaggccg tatcaggcgg cctcgacacc tcagccttcc tcgcctctcc cgcgctcccg
      541 ctcgtcagcg ccgtagccgg gctggttgga gccgccctga ccgtgtggct cgcgggacgc
      601 ttcctcgaca ggcggccctt ctccgagttc gggttccatc tcggggtcgg gtggtggctc
      661 gactttctgt tcgggatggt actcggcgcg ttgctcataa cgatcgtatt cctcgtcgag
      721 cttgggcttg ggtgggtcag gatcaccggc gcgttcgagg catacgggac gggcgctccg
      781 ttcgcgatct ccatgctcct gcccgtcgcg gcgttcctgt gcgtcggctt ctccgaggag
      841 acggtcttcc gcggctacca gctaaagaac gccgccgagg gcctcaacta ccctgccatc
      901 ggtccccgcg gcgccatttt ggtggcctgg gtctcctcct cggtcttctt cggcgtgctg
      961 cacgccgaca accccagtgc cacgcccatc agcactttca acatcgtcct cgcaggcctg
     1021 atgctcggct tcggctacgt actctcggga gagctcgcta tccccatagg tctgcacatc
     1081 acctggaacc tcttccaggg cgcggtctac ggcttcccgg tcagcggctt cgggcctttc
     1141 ggggcgaccc ttctcgccac gaagcaggtc ggcccggacc tctggaccgg gggttctttc
     1201 ggccccgagg gagggctgct cgcccccgcc gtcatgctcc ttgggttctg cctcatagcg
     1261 ctctggacgt acctgcgcac aggtgacttc tcactccact cacgcatcgc cgaaggcccg
     1321 caccgctagc cggatctcca cgggaacgta cttaccttgc gtgctgtacc atacggctcc
     1381 ggctgagctt tgatccatgc atgaaaggat agagtgctgt atcccggcag ccccacaagc
     1441 acggtcatgt gattcgacca gtgacctgac gacaggagga tgggttggaa agagaagtcg
     1501 agaggctggc cggcgggaag atgggcgggg agacccggat cgggaccgtc gcgaccgcca
     1561 gctttattgg caccgctatc gaggcctacg acctctacat ctacggtttc gcggccgctt
     1621 tcgtacttgg accgctgttc ttcccggggt tctcggagac agcccagacg ctcgcggcct
     1681 tcgcgacttt cgccgcctcg ttcctgtcca ggcccctcgg cgccataatc ttcgggcact
     1741 tcggcgacag gctgggccgc aaggctatgc tcgtgctttc gctccttgtt atgggcttcg
     1801 cgacgttctg tataggggtc cttccaggct tcgacacgct gggcgtcgcg gcgcctttgt
     1861 cgctggtcgt gctgcgcttc ctgcagggcg tcgggttcgg aggggagtgg ggcggggcgg
     1921 tcctgatgtc ggcggagcac gccccgcgag gcgggcgcgg attctacgcg agcttccccc
     1981 agctcggccc gcctgtcggg ttcctgttcg ccagcggagc cttcctcctc gtcaccacgg
     2041 ccttctccga cgccgggttc cagtctttcg gctggcggat accgttcctc atcagcatcg
     2101 tgctcgtcgg cgtcggcctc tacgtcaggg tccgcgtcgc cgagacaccg gtcttcaggc
     2161 tggcgatgga ggagcaatcc gcgcctggag tgcccgtcat cgaagtagta cgccgttacc
     2221 cgaaggaact gctcctgacc actggtacca tggtcgtcct cttcggcacc ttctacgtct
     2281 tcagcacctt cgcgccctcc tacgagacga cggagctggg tcttcagacg agcacgacgc
     2341 tctacataac gatgagcgcc ataatcttca tgggcctgac catcccggtc tcggccgctg
     2401 tctccgaccg cgtgggcagg cgcaacctct gcctcgccgc cgctgccctc ttcgggctgt
     2461 gggccttccc catgttctgg ctcatcgaca caggaaatcc gattctgatc atcctcgcct
     2521 tcgccgtgct catggtgatc tacggcatga tgtacggacc agtggccgcc tttttctccg
     2581 agcagttcga gacgcgcgtg cgctacagcg gcatctcgct cggctacaac ctcggcggta
     2641 tcataggcgg cgccttcacc ccgataatcg ccaccgagct gctcacctcg acgggcgcat
     2701 catggtcgat ctcgctctac ctcgtcgccc ttgccgccat ctccttcttc tcggtcttcc
     2761 tcctctcgga gacacactcg aaggatctct cgaagataca aagtcgatag caacgggcgc
     2821 ggtcccgtgg agcgcagccc cgcgacgggc agatcaggga acgcctgacc ccaagtccaa
     2881 taacaaaggc tatccatttg gggcccgatg tgtagccgga tagaacagct aactctggaa
     2941 acaacaaggc ggcccctggc ggaaaggagt ccgtctctcc ctcgcaaagt gagagggtgg
     3001 actgcgccca ccctctcgat tgactccctt ctctttcaaa ttatgggtcg accacaaccg
     3061 tgcatgtgta tgtacctggt gccagatcgt ctgccgtgat cgagccgtca ccgccggcgc
     3121 cggtcgagcc actagtgctg tccgggtcat cgcagctgat ggtcgaagcc gtgccgccag
     3181 gcacctgcga gtcgacggag accgtgatgt cggtcagcgg cgtgttctcg aagctcacgt
     3241 tggtgaaccc cgacgagcaa cttcccgtac tggtcacgtt agccgttttc ggattattgg
     3301 tatccagcgc gtagccggat gctgccacgg tctctgtcac ggtgtacgac ccgagcggca
     3361 gatcgcccag gcagcctatg cccgtatcag ggtcagtcgt gatcgtgcca aacgtcgtcg
     3421 agccctgtgt gacggtgaat gttgcctcca ggttcgggct ggtggccccc gactggtccg
     3481 cgtgcttcgc ggtcttggtc acctggatcg cgccgcaacg gtcgatgttc agcggctcag
     3541 gagcgatgaa gtccttcagc gcagcggtga acgagtcaga cgagcggctc ttgaggtagg
     3601 ccgaaccgaa cccgacgcac gggtcgtcgc cggcgaccgt atcgaaatcg acggtggcct
     3661 cgccaaacgt gcgtggcgag atggctccca gcccgtcgga ctctgatgcc ggaatggccg
     3721 aggtgttgat cgaaccggtg gcgtcattga cgccatctcc gtcgaggtcg gcagcgctca
     3781 ggttgatcct atcgctccag cacggcgtag agttcgcggc ctggcactgg ctcttgtcgc
     3841 cagtggtcac ccactccgac aggaacagct ggggattggt cccgccctgc gcgaggtcgt
     3901 actggatcag gatgtcgccc tcctcgcgga ccggcgtcac gccattcgac gacgtggctg
     3961 tggcgtgatt gaactcgaag tccatgttcg tcgtgccgct cggctcctgc acgcggtgcc
     4021 agtacatgtg gaggaagcgt ccgttggcgt tctcctccag ggtaacgccg aaggtgagca
     4081 ggtcgctctt gttcggcggg atgctaccgt tcaccacggt cggaacggcc gtgtcctcct
     4141 tggtgccctg gccgaacgag ttgtcctggg cgccggtgaa ctcctcggct ttgcggacct
     4201 caaacccatt ggcggagcta ccctgcggga cactcgccca gtcgatggac ggcgctgcat
     4261 cgtctacctt gaggttggcg tcggtgtcga tctcgaagtc gctgcccgct aggctcacct
     4321 cagggtgact tgcctgtgcg gtctgcatgc cagcgagcag cacagccgcc atggcgagtg
     4381 ccccaactat cgcgacgatc gcccaggatc tgagcgggcg tcgctccatc agcgtgctta
     4441 accaccccat catgtcctcc tcctgatgga ggtacatcga tcttgcattc tgttgctcct
     4501 ttcccttcac cgccagaaag ctagtaaatg gcggatcaca gggtagtaga atgcttacgg
     4561 ggtcgggtct ttcttgtatc ggtgcctact cgaaaacaag catccggccc cttggtaatc
     4621 tgagtacctc ttgggctaac cgtatctcta cctccttctc ctacaccagt aggggagcaa
     4681 ggaccctacc tcgtcaggca aggatctcgc cccattcgca gctattgcta cgaacgttta
     4741 g
//
LOCUS       DATAIR010000129         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_422951, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000129 DATAIR010000000
VERSION     DATAIR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422951"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VHM69_03225"
     CDS             <1..376
                     /locus_tag="VHM69_03225"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2739434.1"
                     /translation="MDQGTSAISVDEAKRHGLAREIVEVVLADEKYPLEGHGPWSVGR
                     SQENDIVVNDPNVSRKHARISRADNGFVVEDLGSTNGTLLDGAPIDRERIEGGDELTF
                     GQTTARFIRRIDSPGEESGGRR"
     gene            380..865
                     /locus_tag="VHM69_03230"
     CDS             380..865
                     /locus_tag="VHM69_03230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2739435.1"
                     /translation="MIDLQVPLLAAKALLLLLLFAFIYAVMSRSIGDLRKIPDDEPYV
                     PGQGRNGRGAYARRGSASGSPELVVEDSEILAPETRFEIEDGETSVGRSSGSNIVLKS
                     DDYASGRHAQLTRHGGLLYVEDLGSTNGTFVNGRKTVGATPLRNGDTVRVGSTTFRYE
                     E"
     gene            867..2051
                     /locus_tag="VHM69_03235"
     CDS             867..2051
                     /locus_tag="VHM69_03235"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX2739436.1"
                     /translation="MPFLELQPFGVTDAGKVRQNNEDALLVGEGEDETLFVVADGIGG
                     FEAGEVASSLAVDVLKDLQPDESFKMAIGEANRRILAAGRGDEKLSGMGTTVVAIRFG
                     GTQREPVAEVAHVGDSRAYLMRGGKMDPITEDHSLVAELVRSGDLTRAQAAEHPQKNL
                     ITRALGADEDVDVDTAVLPIEADDRILLCSDGLSDMVPEARISDILAESPNDPERTAR
                     RLLSAALDAGGNDNITVVIVDVKERPSRDDVERAGGEERSGGTSEMPALGPSGRNRGA
                     QSRSRRSAKAARRKRPSGFRKALGKLVRGLAILAVLAIAVTPAYLWGSSRYFFEFDEN
                     GEVVAYRGLPYAPLGVELNQEWRRPGLAESEIKEPYEQPIDNHKLYTRAEAERVLEDL
                     GS"
     gene            2058..3371
                     /locus_tag="VHM69_03240"
     CDS             2058..3371
                     /locus_tag="VHM69_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2739437.1"
                     /translation="MTQRAFTPMILALLVTTLGFATLIFVQEAASPPLIYGAYYAGTL
                     FVLYLGVRLLLPHSDVLLLPIITLLTGIGLVMIFRLTYDIEGAENLAMTQAVWILIGS
                     GTMFLMVLFFRNYYRLFDYKYLLAIAAVFLIASTFTPMGYTVNGARLWVDLGPVNFQP
                     SEFARIALVVFFAGYLAEKRDVMAATSRSVLGVELPAIKYFGPVALVWAASLGLLVFE
                     KDLGSSLLFFAVPLLMLYAATGRIAYVLLGGILFTIGSFVTFLLFDHVQVRVQSWIDP
                     WQDPDGTGFQILQSIFNIADGGLLGTGLGAGFAQTIPEVETDFVFSAIASELGLLGAT
                     AVLLAFLAFTYRGTKIALLAEDDASKLLAFGLTAMFALQTLVIVGGVTKAIPLTGITL
                     PFVSYGGSSVVGNFILTGLLLLVSERAGRREAEGLAYRDDPWGST"
     gene            3356..>3947
                     /locus_tag="VHM69_03245"
     CDS             3356..>3947
                     /locus_tag="VHM69_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2739438.1"
                     /translation="MGVHVNAHLRRTFYLFAAGFVGLVVMLAYWQVYSREALANNPEN
                     GFQTQRSISSPRGLILAGDGETVLAKSVKHHTPAGPIYDRIYPLGEPFSNVVGYWSTR
                     YGATGIEIGYNSDLSSIAGEPQTLDDLINRATGGPQPGNNVVLTLDPELQQLAYQELR
                     ESTTGRGSVVAVDPKTGEILAMATSPSFDPNNIDENY"
BASE COUNT          710 a         1316 c         1246 g          675 t
ORIGIN      
        1 gatggaccag ggtacgtccg cgatctcggt cgatgaggcg aagcgccacg gtctggcgcg
       61 ggagatcgtc gaggtcgttc ttgcggacga gaagtacccg ctggagggac atggtccgtg
      121 gagcgttggc cgctcgcagg aaaacgacat cgtcgtaaac gaccccaacg tgtccagaaa
      181 gcacgcgcgg atctcgcgcg ccgacaacgg cttcgtagtc gaggaccttg gttcgacgaa
      241 cggcaccctc ctcgacggcg cccccattga ccgagaaagg atcgaaggcg gcgacgagct
      301 gaccttcggc cagactaccg cccgcttcat ccgccgcata gactctccgg gcgaggagtc
      361 tggcgggaga cgctagggga tgatcgatct ccaggtgccg cttctggcgg cgaaggctct
      421 gctcttgctc ctgctcttcg ctttcatcta cgcggtaatg agcagaagca taggagacct
      481 caggaagatc cccgacgacg agccctacgt gcccggccag ggccgtaacg gacgcggcgc
      541 ctacgcccgc cgcgggtccg cttcggggtc tcccgagctg gtagtcgagg actcggaaat
      601 cctcgccccg gagacaaggt tcgagatcga ggacggcgag acgagcgtcg ggcgctcatc
      661 ggggagcaat atcgtcctga agagcgacga ctacgcctcg gggcgccacg cccagctaac
      721 caggcacggg ggattgctct acgtcgagga ccttggctcg accaacggga ccttcgtcaa
      781 cgggcgcaag acggtgggcg caacgccctt gaggaatgga gacaccgtca gggtcggctc
      841 gacgaccttc aggtacgagg agtagggtgc ccttcctcga gctgcagccg ttcggcgtca
      901 cagacgctgg caaggtccgt cagaacaacg aggacgccct gctcgtcggc gagggtgagg
      961 acgaaaccct cttcgtcgtc gccgacggca taggcggctt cgaggcgggg gaggtcgcga
     1021 gctccctcgc cgttgacgtg ctcaaggacc tccagccgga cgaatccttc aagatggcca
     1081 taggagaggc gaaccgcagg atcctggccg cgggccgcgg cgacgagaag ctctcgggga
     1141 tgggtacgac cgtggtcgcc atccgcttcg gcgggacgca gagggagccc gttgccgagg
     1201 tggcgcacgt cggggactcc agggcctacc tgatgcgcgg cgggaagatg gacccgataa
     1261 ccgaggacca ctcgctcgtc gccgagcttg tgcgtagcgg cgacctgacc agggcccagg
     1321 cagcggagca cccccagaag aacctcatca cccgtgctct gggcgctgac gaggacgtcg
     1381 acgtcgacac cgccgtcctt cccatagagg cggacgaccg catcctgctc tgttccgacg
     1441 ggctctcgga catggtcccg gaggccagga tctcggacat cctcgccgaa tcaccgaacg
     1501 acccggagag gacggcgcga cgcctgctct ccgccgccct cgacgcgggc ggcaacgaca
     1561 acatcaccgt cgtgatcgtc gacgttaagg agcggccctc acgcgatgat gtcgagcgag
     1621 ccggaggcga ggaaaggtcc ggaggcacca gcgagatgcc ggcactcggg ccttccggaa
     1681 ggaacagggg cgcccagagc cgctcgcggc gatctgcgaa agccgcacgc cggaagcggc
     1741 ctagcggctt caggaaggcg ctcgggaagc tcgttcgcgg gctcgcaatt ctcgcggtcc
     1801 tcgccatagc cgtaacgccc gcgtacctgt ggggctcgag ccggtacttc ttcgagttcg
     1861 acgaaaacgg agaggtggtg gcgtacaggg gtctgccata cgccccgttg ggcgtcgagc
     1921 tcaaccagga atggcgcaga ccaggcctcg cggagtcgga gatcaaagag ccctacgagc
     1981 agcccataga caaccacaag ctatacacca gggcagaggc cgaaagggtc ctcgaggacc
     2041 ttggtagcta gggtatcgtg acccagcgcg ctttcacgcc aatgatcctg gccctcctcg
     2101 tgaccacttt aggattcgct accctgatct tcgtccagga ggcggcgagc ccgcccctca
     2161 tctacggcgc ttactacgct ggaactctct tcgtgctcta cctcggcgta aggctactcc
     2221 tgccccactc ggacgtgctt ctgctaccga taatcacgct cctgacgggc atagggctcg
     2281 tgatgatctt ccgtctgacg tacgacatag agggggccga aaacctcgcg atgacgcagg
     2341 ccgtgtggat ccttataggg agcggcacga tgttcttgat ggtgcttttc ttccgcaact
     2401 actaccgcct cttcgactac aagtacctgc tcgccatcgc cgccgtattc cttatcgcat
     2461 cgacgttcac gcccatgggc tacacggtga acggggcgcg gctgtgggtc gacctcggtc
     2521 ccgtcaactt ccagccctcg gagttcgcac gtatcgcact cgtcgtcttc ttcgcgggat
     2581 acctggcgga gaagcgcgac gtcatggccg cgacgagccg cagcgtcctc ggcgtcgagt
     2641 tgccggcgat caagtacttc gggcccgtcg ccctcgtctg ggcggcgtca ttgggtctcc
     2701 tggtcttcga gaaggacctt ggttccagcc tgctcttctt cgccgtcccg ctgctgatgc
     2761 tctacgccgc gacgggccgg atagcctacg tgctgctcgg cgggatcctg tttaccatcg
     2821 gctccttcgt taccttcctc ctcttcgacc acgtacaggt gcgcgtgcag agctggatcg
     2881 acccctggca ggaccctgac gggacggggt tccagatcct ccagagcatc ttcaacatcg
     2941 cggacggcgg cctgctcggc accgggctcg gcgcgggctt cgcccagacc atccccgagg
     3001 tcgagacgga cttcgtcttc tcggccatag ccagcgagct tggcctgctc ggggccacgg
     3061 cggtcttgct cgccttcctc gccttcacct acagggggac caagattgcc ctgctcgccg
     3121 aggacgacgc gtccaagctc ctggccttcg ggctcacggc catgttcgcc ctccagaccc
     3181 tcgtcatcgt cggcggggtc accaaggcca taccgctcac cggcataacg ctccccttcg
     3241 tctcctacgg cggctcctcg gtcgtaggca acttcatcct gaccggccta ttactgctcg
     3301 tctccgagag ggcggggcgg cgcgaggccg agggtctcgc ctacagggat gacccgtggg
     3361 ggtccacgtg aacgcgcacc tcaggcgcac cttctacctg ttcgctgcgg ggtttgtggg
     3421 gctcgtcgtg atgctcgcct actggcaggt ctactccagg gaggcgcttg ccaacaaccc
     3481 cgagaacggg ttccagaccc agcgcagcat ctcctcgccg cggggtttga tcctggccgg
     3541 cgacggcgag accgtgctcg caaagagcgt caaacaccac acccccgcag gtcccatcta
     3601 cgaccgcatc tacccgctcg gagagccttt ctccaacgtg gtcggctact ggagcacccg
     3661 ctacggggcc actgggatcg aaatcggcta taactccgac ctatcgagca tcgctgggga
     3721 gccgcagacc ctggacgacc tcataaatcg ggcaacgggc ggccctcagc ctggtaacaa
     3781 cgtcgtcctc accctcgatc cggagctcca gcaactcgcc tatcaggagc tacgcgaaag
     3841 cacgacgggc aggggctccg tcgtcgccgt ggacccgaag acgggggaga tcctggcgat
     3901 ggccacctcc ccctccttcg acccgaacaa catagacgag aactacc
//
LOCUS       DATAIR010000130         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_7781, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000130 DATAIR010000000
VERSION     DATAIR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7781"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHM69_03250"
     CDS             complement(<1..215)
                     /locus_tag="VHM69_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2739439.1"
                     /translation="MNGELEAALGAAKAAGEVLRDGFGARRQVRYKGEVDLVTEMDER
                     AEGIIKEALLGAYPSYGMLAEEGGTLPG"
     gene            420..902
                     /locus_tag="VHM69_03255"
     CDS             420..902
                     /locus_tag="VHM69_03255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739440.1"
                     /translation="MVIRHSEPEDYRAVHLVYSSPRAMAETLGVPFASEQVWRKELAR
                     RRDDNFSIVACVRDEVVGHLSLSVYMNPRTRHAGHFGIAVRDDWQGKGVGTELMKACV
                     DLADKWLGLTRLDLRVYVDNEPAISLYKTFGFEIEGTHKRFAFRDGEYVDALVMARLR
                     "
     gene            complement(1010..1363)
                     /locus_tag="VHM69_03260"
     CDS             complement(1010..1363)
                     /locus_tag="VHM69_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739441.1"
                     /translation="MADLKNQIDNFRLLLSSVEAEISKSHEQAALADGAEAWLYMLRE
                     RLAEVEEDSEEGFEARRWLVKLLVSGIAVGRQEDGSPEIKITYRFGPPDGLPEAAEED
                     SNEEMFVGNMENALS"
     gene            1964..2119
                     /locus_tag="VHM69_03265"
     CDS             1964..2119
                     /locus_tag="VHM69_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739442.1"
                     /translation="MDERMRKARELETLERIAAELARLRRLKEHELGVRVENDAGTLN
                     VKPVKKK"
     gene            2294..2737
                     /locus_tag="VHM69_03270"
     CDS             2294..2737
                     /locus_tag="VHM69_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739443.1"
                     /translation="MNRAYQQICPIYPDYATRPIQDGFSWSSLSGCDFDRLYLVVFRS
                     VLRPSADLDLLREHDDRAYEEALNSGGLLRYFKGSANERGECLSFCLWESREQARNAA
                     GAASHRSAASLTGQMYESYVLDRYWLKKVVTALGERLIFEPIQPA"
     gene            2958..3389
                     /locus_tag="VHM69_03275"
     CDS             2958..3389
                     /locus_tag="VHM69_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2739444.1"
                     /translation="MSAEENKALILRVFEEGVNGGDDAVFDEVISPDYVNHSMPAPTP
                     GLEGFKQLIGMFRAAFPDLSVTVVDVVAEGDRVATRGTMSGTHRGDFMGVPATAGTVE
                     VPYIDIWRVEGGKGVENWVQMDMLGIMQQLGVVPMPEQATS"
BASE COUNT          714 a         1084 c         1095 g          713 t
ORIGIN      
        1 ccaggcagcg tacccccctc ctccgcgagc atgccgtacg acggatacgc cccgagcaga
       61 gcctccttga tgatcccctc ggccctctcg tccatctccg tgaccaggtc cacctcgccc
      121 ttgtacctga cctgacgacg cgccccgaat ccatctcgaa gcacctcgcc ggccgccttc
      181 gccgccccca gcgccgcttc caactctccg ttcaaagctc gaaactcctt ccgtacaatt
      241 tcgtcctcgc ttagtagtac ccgctgtcac gagtagttaa ttcagcggga catccgctcc
      301 tttcagtacg ccctgggacc catagcttcg gcaggcatac acaagcgcaa tagcgagctg
      361 ccacagactc gtgcgaagat accggcctat aaaagcgaag gagcatctgt gtgagtgata
      421 tggtaattcg acactccgaa ccggaggatt acagggcagt tcacctcgtt tactcctccc
      481 cgcgggctat ggccgaaacg cttggcgttc ccttcgcctc agagcaggta tggcgcaaag
      541 aactggcccg ccgacgtgac gacaacttct ccatcgtagc ctgcgtacgc gatgaggtcg
      601 tcgggcatct ctcgctcagc gtctacatga accctagaac acgacatgcc ggacacttcg
      661 gaatagcggt gcgggatgac tggcagggaa agggtgtcgg taccgagttg atgaaggctt
      721 gcgtggacct cgcggacaaa tggttgggcc ttacgaggtt ggatctccgg gtatacgtcg
      781 ataatgagcc cgcgatctcc ctctacaaga cgttcgggtt cgagatagaa ggaacacata
      841 aacggtttgc cttccgcgac ggcgaatacg tagatgctct cgtcatggcc cgtctacggt
      901 gagggtcact ctagtgagat tgcgatcctg cggcacaggg aatgatctac gacacgcaaa
      961 cgcagtaagg gagtagtgcc atgtcccagt gcgaagtctg cggaaacgac tacgacaagg
     1021 cgttctccat gttacccaca aacatctcct cgtttgaatc ctcttccgcc gcctccggta
     1081 ggccgtctgg cggcccgaag cggtaggtaa tcttgatttc ggggcttccg tcttcctgcc
     1141 ggcctaccgc aatgccggag acgaggagct tcacgagcca acggcgcgcc tcgaaccctt
     1201 cctcagaatc ctcctccact tccgccaggc gttcacgcag catgtagagc caggcttcgg
     1261 cgccatcggc gagcgccgcc tgttcgtggc ttttggatat ctcggcctcg acagatgaga
     1321 gcaggagccg gaagttgtcg atctgattct tgaggtccgc catataaact gccaactcct
     1381 cctcggagat gtgcgcttga gcgtacagac ggacgtagcg gtccttctca gcctgcttcg
     1441 cggccagcgc ggagcgggcg cgctcctgta cactatcgct ggagtgtgga gcctgtcgcg
     1501 atattcagaa ggatctcgcg cactacctcg gtctcctcgg gcacgatctc agcttgtgca
     1561 gtgtggccga cgctctgctg ctcgacccgc tgatcctggc tctcacggat gccaaggtac
     1621 gggcagagga ggagcttgag cgcctggagg atcgtctctc aagaccagac ggtgagcagc
     1681 tgcatccgct gcgaggagga gcgcctgcga gggcttctga ggcgctgtga cgtgatcgga
     1741 aacgctcaca ccatcgtcct cgacgcggag gggatatacc cagaggcgaa agcacgtacg
     1801 ctggaaggcc tggcggagat gcgcaaggag gcaggcgagc agttcagcct ctacaagcaa
     1861 gaattcggca tctccgatcg gagcgccagc tcatcgtgtg attacatcgg gccggggcta
     1921 tccttctcca ggtgtcccgg ccctttactt tgggaggaga gaggtggacg agaggatgcg
     1981 caaggcccgc gaactcgaaa ccttggagcg cattgccgcc gagctagcgc ggcttaggag
     2041 gctaaaggaa cacgagctcg gcgtgcgggt cgagaatgat gcgggaacct tgaacgtaaa
     2101 gccggtcaag aagaagtagc cgcagacgag ccggggtcct ctacggggct ccggcctttg
     2161 tgcgtggcat acacaaagat atgccgctag atacgccatg tggcagatgc ggcggcctct
     2221 gtacctcttc taagatgccc tcgaggtcgt gaacacagtc gatgtccgca gcgaagcctt
     2281 agaggtgcct gtcgtgaaca gagcctacca acaaatctgc cccatctatc cagactatgc
     2341 aacgcggccc atccaggacg gctttagctg gtcttccctc tcaggctgcg acttcgaccg
     2401 cctttacctg gtcgtcttcc gctccgtcct tcggccgagc gccgacctcg acctcctgcg
     2461 cgagcacgac gaccgcgcgt acgaggaggc tctgaactca ggcggcttgc tgcgctactt
     2521 caaagggagc gcgaacgagc gtggggagtg cctctccttc tgcctgtggg agagccgcga
     2581 gcaggcaagg aatgctgccg gagccgcctc gcaccggtcc gctgctagcc ttaccgggca
     2641 gatgtacgaa tcctacgtgc tcgaccgcta ctggttgaag aaggtcgtaa ctgcgctagg
     2701 agagaggctc atcttcgagc cgatccagcc ggcgtagatg agtcagagat gaaaggcggc
     2761 attgctggcc cgtcaagagg caagggccgt gtgctgcgtc tagtgtagat aagctggtcg
     2821 ggccggggta tcctttgggg tgtcccggcc gttctgtatc ctcggtaaaa tcatccattt
     2881 gggtgatccc gccgcttacg ccaggcgtct atcatggcac cgtaagcaaa cccgctagga
     2941 aagcgaggag gctgagcatg tctgcggaag agaacaaagc ccttatcctc agggttttcg
     3001 aggagggcgt aaacggaggc gacgatgccg tgttcgacga ggtgatctct ccggattacg
     3061 tcaaccacag catgcccgct cccacgcctg gcctcgaggg cttcaagcag ctgatcggca
     3121 tgttccgcgc cgcgttcccc gacttaagcg tcacggtggt ggacgtggtg gccgaaggtg
     3181 acagagtggc gacccgaggg acgatgagcg gcacccaccg gggagatttc atgggcgttc
     3241 ccgccaccgc aggaacggta gaggttccgt atatagatat ctggcgagtc gagggcggca
     3301 agggcgtgga gaactgggtg cagatggaca tgctgggcat tatgcagcag ctcggcgttg
     3361 tccctatgcc tgagcaggct acgagttaac ctctgcagac atctcacgaa gcgctaggcc
     3421 ggggtcctac ggggctccgg ctctttcgcg cccggccttc gctttgccgc gtacccctga
     3481 agcgccacta ccttgccggc ggtcctgggc cgcctggacc ggggctatgg gcttcgtatt
     3541 gggagttcgc cgaggttcgg cgctttactc gcagttggtg actgtgtcca aaccagcatc
     3601 gaaagt
//
LOCUS       DATAIR010000131         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_284944, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000131 DATAIR010000000
VERSION     DATAIR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284944"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="thyX"
                     /locus_tag="VHM69_03280"
     CDS             complement(<1..828)
                     /gene="thyX"
                     /locus_tag="VHM69_03280"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2739445.1"
                     /translation="METTERNTERARQLFEEFGGSVTDGFPAIHSPAYHTEAGTPYLK
                     RPGVVVLARPQTNVAGLGGFLEGFDPALRFPEYIEDPTELPDSTQLCKTAGQTCYMSF
                     GPRRTTNENAAAYIERLTSAGHGSVLEHASFSFLLYGISRSVTHELVRHRAGTGFSQI
                     SQRYVSGSVLRFVERPEYGEDEELHRLFEERADMAAAGYEEMAQRLLERQEGGASMLT
                     ADFKTDARKKVQQTARSLLPNETEAPMVFTGNVRALRHIIEMRADAHAESEIRNLALR
                     "
     gene            complement(885..1727)
                     /locus_tag="VHM69_03285"
     CDS             complement(885..1727)
                     /locus_tag="VHM69_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2739446.1"
                     /translation="MMEAQDVLTQYMGRVRGGRLLDAGEEKDLARRARGGDMRARQKL
                     IESNLRLVISIAKKYRGRGVLFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAVADHARTVRLPAHVVDAIYRLRRTENTLSIELGRDATEEEVVESLGVKPEEAR
                     RLREVSQPISSINARINTDEGSEMGELLPDEHSGDDYTRVEVGQWEVALVEAVRSLPE
                     REARIIEMRHGLDGSDTRTLREVSEELGISQERARQVEIKALRTIRTGRYASTLRRAL
                     SEAV"
     gene            complement(1758..2051)
                     /locus_tag="VHM69_03290"
     CDS             complement(1758..2051)
                     /locus_tag="VHM69_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739447.1"
                     /translation="MGGKSVRGRTRRSALRPDTALIPVGEPEREIYVWSLPACCLRSL
                     KEKAMAEAGGRRRLELECSCGRSWRVTSSLDGRILDRFVTHGGPRVGGSYPAA"
     gene            complement(2067..2243)
                     /locus_tag="VHM69_03295"
     CDS             complement(2067..2243)
                     /locus_tag="VHM69_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739448.1"
                     /translation="MYGIADLEVSRLHREEIERDIRFNRMSGGRGGGFRVMLELKRDF
                     GLFLRLFQTAKNAG"
     gene            complement(2271..>2487)
                     /locus_tag="VHM69_03300"
     CDS             complement(2271..>2487)
                     /locus_tag="VHM69_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739449.1"
                     /translation="EGRIVGGSVWVAALGQEVLYEEDLPACCRRSARDGDRTFECPAC
                     GAMWQEALPVEPEQDAFAERDERRGAA"
BASE COUNT          383 a          872 c          734 g          498 t
ORIGIN      
        1 gcgcagggcg aggttgcgga tctcgctctc cgcgtgcgcg tctgcccgca tctcgatgat
       61 gtgccttaga gcgcgcacgt tccccgtgaa gaccatcggg gcctcggtct cgttcggcaa
      121 caaggaccgc gcggtctgct gtaccttctt cctggcgtct gtcttgaagt cggcggtgag
      181 catgctcgcc ccgccctcct ggcgctccag caggcgctgc gccatctcct cgtagcctgc
      241 ggcggccatg tcggcccgct cctcgaagag cctgtgcaac tcctcgtcct ccccatactc
      301 aggacgctcc acgaagcgca gtacacttcc cgaaacatag cgttgagaaa tttgggaaaa
      361 acctgtgcct gccctgtgac gcacgagctc gtgtgtgacg ctgcggctga taccgtatag
      421 caggaaactg aagctcgcgt gttcgagtac ggagccatga cccgccgagg tcagccgctc
      481 gatgtacgcg gcggcgttct cgttcgtcgt tcgccggggt ccgaatgaca tgtagcacgt
      541 ctggccggcc gtcttgcaga gttgggtgga atccggcagc tcggtggggt cttcgatgta
      601 ctcggggaag cggagcgcgg gatcgaagcc ctccaggaaa ccgcctagtc cggccacgtt
      661 ggtctgcggc ctcgccaaca cgacaacgcc tggtcttttg aggtaagggg ttcccgcctc
      721 cgtgtgatac gccggtgagt ggatggccgg aaagccgtcc gtgacgctcc cgccgaactc
      781 ctcgaatagt tgccgcgccc gctcggtgtt acgctccgta gtctccaatg ggctcccttc
      841 tccgtagtgc tgctagagag caggttagcc catttggctc ggttctacac ggcctcagat
      901 agagcgcggc gaagggtgct ggcgtagcgg ccggtacgga tcgtccttag agctttgatc
      961 tcgacctgcc tggctctctc ctgggagatg ccaagctcct cggagacctc gcgcagggta
     1021 cgggtatcgc tgccgtcgag gccgtggcgc atctcgatga tcctggcctc gcgctcgggc
     1081 agcgacctca cagcctcgac gagtgccacc tcccactggc cgacctcgac gcgggtgtag
     1141 tcgtcgccgg agtgctcgtc gggcagaagc tcgcccatct cggacccttc gtcggtgttg
     1201 atcctggcgt tgatgctgga gatgggctgg ctgacctcgc gcaggcgtct ggcctcttca
     1261 ggttttacgc ccagactctc gacgacctcc tcctcggtcg cgtcgcgtcc gagctcgatg
     1321 ctcaacgtgt tctcggtgcg gcgcaagcgg tagatggcgt caacaacatg cgcaggcagc
     1381 cttacggtgc gtgcgtggtc ggcgacggcg cgggtcacgg cctgccttat ccaccacgtc
     1441 gcgtaggtcg agaaacggtt gcccatctcg gggtcgaact tctcgacggc ccggatcagg
     1501 cccgcgttcc cctcctggat gaggtcctcg aagagcacgc cgcgccccct gtatttcttg
     1561 gcgatggaga tcacgagccg cagattggac tcgatgagct tctgacgggc cctcatgtct
     1621 cccccgcgtg cgcgcctggc gagatccttc tcctcgcctg cgtcgagcag cctccctcct
     1681 cgcactctgc ccatatactg tgtgagaacg tcctgtgcct ccatcatcgt ctatcccctc
     1741 tcttagatct caggtattca tgccgccggg tacgagcccc cgacccttgg gccgccgtgc
     1801 gtcacgaagc ggtccaggat gcgtccgtcg aggctcgatg tgacccgcca gctccgcccg
     1861 cacgagcact ccagctcgag gcgcctccgc ccgcccgcct ctgccatggc cttctccttg
     1921 agtgagcgca ggcagcacgc aggcaatgac cacacgtaga tctcccgctc aggctcgccc
     1981 acagggatga gcgcggtgtc gggcctgaga gcgctccgcc ttgtgcgtcc ccgcaccgat
     2041 tttccaccca ccttggatct ccctcactaa cccgcgttct ttgcagtctg aaagagcctg
     2101 aggaacagac cgaagtctcg ctttagctcc aacatcacgc ggaaaccccc gccgcgccct
     2161 cccgacatcc tgttgaacct gatgtcgcgc tctatctcct cccgatgtaa cctcgatact
     2221 tcgagatccg ctatgccgta catccccaaa acctcactcc cgtcgtgttc ctacgccgct
     2281 ccccgacgct cgtcacgttc cgcgaaggcg tcctgctctg gctccacggg caacgcctcc
     2341 tgccacatcg cgccgcaggc cgggcactcg aacgtgcggt cgccgtcccg cgcgctgcgc
     2401 ctgcagcagg cggggaggtc ctcctcatag aggacctcct ggccgagggc agccacccag
     2461 acagagccac ccactatgcg gccctcc
//
LOCUS       DATAIR010000132         5747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_100250, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000132 DATAIR010000000
VERSION     DATAIR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100250"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="mutM"
                     /locus_tag="VHM69_03305"
     CDS             <1..718
                     /gene="mutM"
                     /locus_tag="VHM69_03305"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2739450.1"
                     /translation="VDPEEFGRRLVGRTLKSTGRRGKIIVLDFGEVVGLVHLVISGRV
                     LRLPEWKEPDRMNTAVLEFDGEPLVLAFTRLWLGYFDLYEPGEVENHPLISRLGPDPF
                     SAEFTVDYLASAFGRKASVKGLLLDQSVVAGLGNIYVDEVLFAAGVHPTRKAATLDIE
                     EVSKVHAATLDILGRAIEQRGTTFDSYHDAFGETGKYQRQLMVFDRAGEPCLECGTEI
                     VKSRVAGRGTHTCPSCQNVS"
     gene            complement(907..1494)
                     /locus_tag="VHM69_03310"
     CDS             complement(907..1494)
                     /locus_tag="VHM69_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739451.1"
                     /translation="MMHMINRLAPAVVIVLSAFMLAGCSGMFGEDPRDQANKAISDAN
                     KSISEHNKLFDEARSTYADVKQKIESGDDPSKEKGRITEAKRALEEARKDLQDASDSL
                     DSVKDLDVDPTIKKYAGLLSEAMDAQLAAEAKEIEFYGILEKDPALEDNREKALDLLS
                     EVGEGYQEAERTYADAQDLANSHPKLIEAANKAES"
     gene            1562..1909
                     /locus_tag="VHM69_03315"
     CDS             1562..1909
                     /locus_tag="VHM69_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739452.1"
                     /translation="MLGRLFGRRRRREEGPDPALISLAASAISEYARDHTGLFERAER
                     LRNKADRLESAGIPSESAANRAERATREVEAGLLALRASFVASAGGVAGESAFDHEVE
                     RLYPTLGISGRQP"
     gene            complement(1948..2406)
                     /locus_tag="VHM69_03320"
     CDS             complement(1948..2406)
                     /locus_tag="VHM69_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739453.1"
                     /translation="MFGRKSRAEKLKEQAESNSLIPVATLATAYAGAKPVIERLLYDD
                     DLRDNIRIFIESARSIIDELSGEDPSDIVTRLWDDDKLRRHVEAAAGAASQGSKRVRG
                     EKVRERGGRGGRLLFLLLVGAVGFLFLHPKTGPQARRFAMDAYGSLTSGE"
     gene            complement(2453..5641)
                     /gene="ileS"
                     /locus_tag="VHM69_03325"
     CDS             complement(2453..5641)
                     /gene="ileS"
                     /locus_tag="VHM69_03325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX2739454.1"
                     /translation="MKFDKVSPQVDFPRLEEGILDLWKKSGVFEKSVQRRPEDRPFVF
                     YEGPPTANGTPGFHHALARSFKDLIPRYKTMQGYRVERKGGWDCHGLPVELQVEKELG
                     LEGKADIERYGVEEFNRLCRESVFRYVESWQKMSDRLGFWVDMDDPYQTLEDSYIESV
                     WWALKTLHEKDLLYEGFKVSPHCPRDQTSLSSAEVALGYQQYPDPVVDPSVYVRMPLE
                     DGTSLLIWTTTPWTLIANAAVAANPDVKYAAVQVGDERLILAEDLLGKVLGEVDYEVL
                     STTRGRNLEGKRYRRPFDHVPVEESENLWTVVLGEHVTTTEGSGLVHTAPAYGEEDAR
                     IGRKYGLPTIHPVRTDGTFDERVGPFAGQFVKDADAGLVEELRDKGLLFKAEELEHAY
                     PHCWRCGTPLLYYAKKAWYGRTTAVLDQLLTENQEINWVPEHMKWGRFGDWLKNNIDW
                     ALSRERYWGTPLPIWRTASGATVVVGGVEELKDLAIDPVPEDLHRPHIDRVRIRHPET
                     GEEATRVPEVLDAWFDSGSMPFAQWGYPHYEAGEERFRRQFPADFICEAVDQTRGWFY
                     SLLAVSTMLFGKGSYRNCLVLGHILDAEGKKMSKSLGNVIDPWDVFEKQGADALRWAF
                     YTATSPGNTRRFSREQVDEAVRKYLLTLWNTYSFFVTYARIDGFDPKSDFVPPEERSL
                     MDRWALSEMQLTIRTVTQKLDAYDVTAAGRAIGEFVDELSNWYVRRSRRRFWKGEDDQ
                     DKKAAHSTLYECLVAVAKLTAPFTPFVAESLYQNLVVNADETAPESVHLADWPEYGEE
                     LVDQSLSERMSAARRVVGLGRAARNAAAIKTRQPLREVVVVDETSDAIGVKEGVQSLR
                     EIVLDELNVKDLAFGEAEDVVAYDLKPNLGVVGPKYGKLVPGLRAALAEAAPEVGARA
                     AAGESVSVSVEGEEISLSPDELLVEPREREGYALEREGGLSVALRTQLDSELVDEGLV
                     RELVHRVQNLRREKGFEIEESIRVGLSGNKRISNLLEGHWGDYFRAEVLARELDLDPN
                     TPDGDYESVTVDGEALWVRVEPLGGRGPGKRV"
BASE COUNT          962 a         1862 c         1759 g         1164 t
ORIGIN      
        1 cgtagatccc gaggagttcg ggcggcgact cgtcggacgg acgctgaaga gcaccggacg
       61 gcggggcaag atcatcgtcc tcgacttcgg ggaggtcgtc gggctcgtgc atcttgtgat
      121 ctccggaagg gtgttgcgcc tccccgaatg gaaggagcca gacaggatga acaccgcggt
      181 cctcgagttt gacggggaac cgcttgtact agcattcacc cggctctggc tcgggtactt
      241 cgacctgtac gagccgggcg aagtagagaa tcaccctctg atctcacgtc tcggccccga
      301 ccctttttcc gcggagttca ccgtcgatta ccttgcctcg gccttcggca ggaaggcgag
      361 cgtgaagggt ttgctcctcg accagtcggt cgtggcgggc ctcggcaata tctacgttga
      421 cgaggtcctc ttcgccgccg gagtccatcc cacgcgcaag gccgctacgc tggacataga
      481 agaggtcagc aaagtccacg ccgccaccct ggacatcctc ggccgcgcca tcgagcagcg
      541 cgggactacc ttcgactcct atcacgacgc attcggggag acgggcaagt atcagcgcca
      601 gctcatggtc ttcgaccggg cgggcgagcc ttgcctcgag tgcggcacag agatcgtcaa
      661 gtcgcgcgtc gcaggaaggg gaacccacac ctgcccctcg tgtcaaaatg tcagctagtc
      721 agcacgctac gccctatggg cttcgcttgt cagcaattca gcacgccgcc tgacggcggc
      781 tctcagcatt tcagccgggt tgtcacagtc acacgaggct cccgagcgga tgaagctgaa
      841 ggctgaaagc gagggtcgcc aggcccgaag cgagctgaaa ggcgcccgcc gtgctgacaa
      901 gctgtttcaa gactcggctt tgttggcggc ctcaatgagc ttgggatgag agttggcgag
      961 gtcctgggca tcagcgtagg tcctctcggc ttcctgataa ccttctccga cctcggagag
     1021 caggtcgagg gccttctccc gattgtcttc gagggcggga tctttttcca ggatgccgta
     1081 gaactctatc tccttggctt cggcggcgag ctgggcgtcc atcgcttcag agagcaggcc
     1141 ggcgtacttt tttatcgtag ggtccacatc gaggtctttc acggaatcga gcgagtcgct
     1201 ggcgtcctgt aggtcctttc gtgcctcttc gagggcgcgc ttggcctccg tgatgcggcc
     1261 cttctccttc gaggggtcgt cgccggattc gatcttctgc ttgacgtccg cgtaggtaga
     1321 gcgtgcctca tcgaagagct tgttgtgctc ggagatggac ttgttcgcgt cgctgatcgc
     1381 cttgttggcc tgatcgcggg gatcctcccc gaacatgccg gagcacccgg caagcatgaa
     1441 tgccgagagc acgattacaa cagcgggcgc cagacggttt atcatatgca tcatgagcga
     1501 aagtagccta cctcttttag ggtctctcag aatacgttcc ttcccttcaa ctgccaggcg
     1561 tgtgctcggc cggctgttcg gtcgcaggcg gcgtagagaa gaagggcctg acccggcgct
     1621 gatctcgctg gcggcttccg ccatcagcga gtacgcccgg gatcacaccg gcctcttcga
     1681 gcgggcggag cgactgcgga acaaggccga caggctggag agtgccggga taccgagcga
     1741 gagcgccgcc aatcgcgcgg agcgggccac gagagaggtc gaggccgggc tcctcgccct
     1801 ccgggcttct ttcgtcgcgt cggcgggagg ggttgccgga gagagcgcct tcgaccacga
     1861 ggtcgaacgc ctttacccga cgcttggcat ctccggccgt caaccctgac ccccctcaaa
     1921 gtctccgtct ggcggcttcg tccagcttta ttcgccggaa gtcagcgagc cgtaggcgtc
     1981 catggcgaac ctgcgtgcct ggggacctgt cttcgggtgc aggaaaagga agccgacggc
     2041 cccgacgagt aacaggaaca gcagcctgcc gcccctgccg ccacgctcgc gcaccttctc
     2101 gccccgaacc cgcttgcttc cttgactggc cgcccccgcg gcggcttcga cgtggcggcg
     2161 cagcttgtcg tcgtcccaca gcctggtgac gatgtcgctg gggtcctcgc ctgagagctc
     2221 gtcaataatg gagcgggccg actcgatgaa gatgcggatg ttgtcccgaa ggtcgtcgtc
     2281 gtagagtaga cgctcgatca ccggcttggc tcccgcgtaa gccgtggcca gcgtcgccac
     2341 aggaataagc gagttggact ctgcctgctc ttttagtttc tccgcccggc tctttctgcc
     2401 aaacacgcga aacctccctc tcctggttcc tttcgtatct ctgatccgta catcataccc
     2461 gtttccccgg gcctctaccc ccgagcggct ccaccctgac ccacaaagcc tccccgtcga
     2521 ccgttacgct ctcgtagtcc ccatcaggag tgttcgggtc gaggtcgagc tccctggcaa
     2581 gcacctcggc ccggaagtag tcaccccagt gaccctctag tagattcgag atcctcttgt
     2641 tccccgagag gccgacgcgg atgctctctt ctatctcgaa gcccttctcg cgcctcaggt
     2701 tctgcaccct gtggacgagc tctctgacca gcccctcgtc gacgagctcc gagtcgagtt
     2761 gcgtgcgcaa agcgacggag agtccgccct cgcgctctag ggcgtaccct tctctctccc
     2821 tgggctcgac gagcaactcg tcgggggaga gtgagatctc ctccccttcc acgctcacgg
     2881 atacgctctc tccggcagca gcccgggcac cgacctcggg cgcagcctcg gcgagggcgg
     2941 cacgaagtcc tggcacgagc ttgccgtatt tcggaccgac gacgccgagg ttgggcttga
     3001 ggtcgtaggc gaccacgtcc tcagcctccc cgaaggcgag atccttgacg ttcaactcgt
     3061 cgagtacgat ctcccgaagg ctctgtacgc cttctttgac tcctatggca tccgaggttt
     3121 cgtcgacgac gacgacctct ctcaaaggct ggcgggtctt tattgcggcg gcattgcggg
     3181 cggcccgccc gagacccacc acgcgccttg ccgctgacat ccgctccgag agactctgat
     3241 ccaccagctc ttccccgtac tcgggccagt cggcgaggtg cacgctctcc ggggccgtct
     3301 catccgcgtt cacgacgagg ttttggtaga gggattcggc gacgaatggg gtgaaggggg
     3361 ctgtgagctt ggcgacggct acaaggcact cgtagagggt ggaatgggct gccttcttgt
     3421 cctgatcgtc ctcccccttc cagaagcgcc tgcggctgcg cctgacgtac cagttggaga
     3481 gctcatccac gaactccccg atggccctgc ccgccgcagt cacgtcgtag gcgtcgagct
     3541 tctgtgtcac ggtccttatc gtgagttgca tctcgctgag cgcccagcgg tccatcagac
     3601 ttcgctcttc gggcgggacg aaatccgact ttggatcgaa gccgtcgatg cgcgcgtagg
     3661 tcacgaagaa agagtaggtg ttccagagtg taaggaggta cttgcggacg gcttcatcga
     3721 cctgttccct ggagaaacgc cgcgtattgc ccggactcgt ggctgtgtag aacgcccagc
     3781 gcagggcgtc tgcgccctgc ttctcgaaga cgtcccacgg gtcgatgacg ttgccgagcg
     3841 acttgctcat cttcttgccc tcggcatcca ggatgtgacc gaggaccagg cagtttctat
     3901 acgagccctt gccgaacaac atcgtcgaga cggcgagcaa cgagtagaac cagccccgcg
     3961 tctggtcgac ggcctcgcag atgaagtccg cagggaactg ccgcctgaaa cgctcctcgc
     4021 cggcttcgta gtgtggatag ccccactggg cgaaaggcat gctccccgag tcgaaccacg
     4081 cgtcgaggac ctcggggacg cgggtggcct cctcgccggt ctcggggtgt ctgatacgca
     4141 ccctgtctat atgcggcctg tgcaggtctt cggggacggg gtctatggcg aggtccttca
     4201 gctcctcgac gcctccgacc acgaccgtcg cgcccgaggc ggtgcgccag atcgggagcg
     4261 gcgtccccca gtagcgctcg cggctcaagg cccagtctat gttgttcttg agccagtctc
     4321 caaagcggcc ccacttcatg tgctcgggga cccagttgat ctcctggttc tcggtgagaa
     4381 gctggtcgag cacggcggtc gtccgtccgt accacgcttt cttggcgtag tagagcaatg
     4441 gtgtcccgca ccgccagcag tgagggtaag cgtgctccaa ctcctccgct ttgaacagga
     4501 gtcctttgtc cctcaactcc tccacgagcc ccgcgtccgc gtccttgacg aactgcccgg
     4561 cgaaaggccc gacccgctcg tcgaacgtgc cgtctgttct gacagggtgg atggttggca
     4621 ggccgtactt gcgtccgatg cgggcgtctt cctcaccata cgccggcgcg gtgtgcacga
     4681 gtccggaccc ttccgtggtc gtcacgtgtt ccccgagcac gactgtccac aggttctccg
     4741 actcctccac gggcacgtga tcgaagggac gacggtacct cttaccttcg aggttccgcc
     4801 ccctcgtcgt ggaaagaacc tcgtagtcga cttctccgag caccttcccg aggaggtcct
     4861 cggccaggat cagtcgttcg tcgccgacct ggacggccgc atatttcaca tcagggtttg
     4921 cggcgacggc ggcgttggcg atgagggtcc acggagtcgt ggtccagatc aggaggctag
     4981 ttccatcttc caggggcatc ctcacgtaga cgctcgggtc tacgacggga tctgggtact
     5041 gctggtagcc cagagctacc tcggcggagg agagggaggt ctggtcgcgg gggcagtgag
     5101 ggctgacttt gaaaccctcg tagaggaggt ccttctcgtg gagggtcttc agggcccacc
     5161 agacgctctc gatgtatgag tcctcgagcg tttggtaggg gtcgtccatg tccacccaga
     5221 agccgagccg gtcactcatc ttctgccagc tctccacgta gcggaagacg gactcgcggc
     5281 acaggcggtt gaactcctcg acgccgtagc gctctatgtc ggccttgcct tcgaggccga
     5341 gctccttctc tacctgaagc tcaacgggca gaccgtgaca gtcccagccg cctttgcgct
     5401 cgacgcggta accctgcatg gtcttgtagc gggggattag atctttgaaa gacctcgcga
     5461 gcgcgtggtg gaaccctggt gtgccgttgg ccgtcggcgg cccctcgtag aagacgaagg
     5521 gacggtcctc cggccttctc tgcacggatt tctcgaagac tcccgacttc ttccagagat
     5581 caaggatccc ctcctccagc ctcgggaagt ctacttgcgg gcttactttg tcgaacttca
     5641 cgtcgcggac ctcctcacac aaaagctccc acccctcggg cggagcgtcg ctccaccgta
     5701 gggacgggag cctgcacaag gctctcgcgg taccacccta cttgccc
//
LOCUS       DATAIR010000133         6453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_234362, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000133 DATAIR010000000
VERSION     DATAIR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234362"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1815)
                     /locus_tag="VHM69_03330"
     CDS             complement(238..1815)
                     /locus_tag="VHM69_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739455.1"
                     /translation="MKRMAIVRLTAMPALALLGVLLLALLSGVADAATTFTVNKSSDA
                     KDRRISDSVCDTSRNKGKQCTLRAAIQESNDTSGADTIKFNIGGTASVKTINVGSSGL
                     RALPDITGPVTINGYTQPGASENTLATGNDAVLKIQLNGTDAGFGANGLKITASNSTI
                     KGLVINSFFDNGVQIAGSGATGNKIEGSFIGTNAAGTGALANGNAGVDISNAPNNTVG
                     GTQPAQRNVLSSNVADGVQIAGSLATGNKVQGNFIGTSASGTQDLGNSGDGVVIDIAP
                     NNTVGGTASGARNVISGNRFGVFISGSGATGNKVEGNRIGTKADGSGDLGNDGDGVII
                     TAGASNNTVGGTTSGAGNAIAGNGDDGVSIVEGRGNSVLSNFIFSNTGLGMNLFPDGV
                     TANDTDDPDTGANNLQNFPVIASAIKSSTSPFFTTITGTLNSNPNQTYTIQCFVAVPD
                     PSGHGEGQIPVGQDTIQTDAGGDADGTDSFSCASPVPQLGQAVTATATNTSGTGSGTS
                     IGDTSEFSLNVGVSPGP"
     gene            complement(2133..4412)
                     /locus_tag="VHM69_03335"
     CDS             complement(2133..4412)
                     /locus_tag="VHM69_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2739456.1"
                     /translation="MSNFIGREAEILEIKRHLSMTRLLTLTGTGGVGKTRLALEVAGD
                     LTTVYPAGVWLVEFAPLSDPDLAPQAVAAALGVREEQGRPLFTTLVEELRSSELLLVL
                     DNCEHLVGACARLADTLLSSCAGLRVLATSREPLGITGEVCWPVRSLSMPDVNYRPTV
                     EHLAGYESVRLFVDRARTRLPGFELTSANSGAVAKICRKLDGLPLAIELATARMTVLA
                     IEQVAVRLDDALELLTGGSRTADPRHQTLRAALEWSYEPLSDRERRLFERLSVFAGGW
                     TLEAAEAVGIGDGIESADVLDLLGRLVSKSLVTLHTGSLDTARYGMLEPVRQYARERL
                     DAARHERARERHAMYFLTLAEEAEPGLLGPRPGPWLERLSEERDNLRAALSWALDAED
                     RAEAGLRISVALVRFWGVFGVSEGRRWLEKALSRSRALRTPLRARALNETGFLILFGG
                     GFEQAIAMLEESLALSRELKDSSSAAGAVRSLVYALAHAGDSVRVAQLREEAEALVRG
                     PLDQRSRAGLLEALTLAALDAFDYEGALDYAAEAYAAYRALGDVQGTVVALAALGMVS
                     LTAGNPEQAQAYLAENLELLRGMGHKMGIAYCLLGMAGVAGERARPDRAARLWGAAEA
                     LREAIGMHLTPFDRSHYRYEERLAAARALIEEDAWERVWAEGRAMSPEEAIEYALAEE
                     GAFRIPERPTAGHAEVLTPREREVAEEVAKGLTNRQVATELGISERTVHAHVRRILRK
                     LSLGSRAQIAAWVIEQRGS"
     gene            complement(4409..5302)
                     /locus_tag="VHM69_03340"
     CDS             complement(4409..5302)
                     /locus_tag="VHM69_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2739457.1"
                     /translation="MTSFREAAPGSAAPLVRGNGDAPAADLEPLRIGLLGGFQVRVGS
                     RVVGENEWRLRKAASVVKLLALAPGHHLHREQAMDLLWPDLGAKAAANNLHYALHVAR
                     GVLDPGPRAPAGYLRLRGGRLQLCPESPLWVDVEAFEEAAATARREREPAAYRAALDL
                     YAGDLLPEDRYGVWTEERREELRQLYLALLVELARAYEERGERERAILSFERVVTEQP
                     IHEKAHTGLMRLYAAAGHPVKAIIQYERLKETFRHQLDRTPDNATRRLYRRILAQQPP
                     TARPPAPEKHSTTVGTTCRLP"
     gene            5801..>6453
                     /locus_tag="VHM69_03345"
     CDS             5801..>6453
                     /locus_tag="VHM69_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2739458.1"
                     /translation="MNTAVHREFKDRLFEQFARIGKALASPKRLEIVDLLAQGERTVE
                     EISRETAMPVANASQHLQALKGVRMVESRREGLYAYYRLADEGVFLTWQAVRALGETR
                     LAEVDRVVETYLEDRGALEAVEATELMERLGDGSAVVLDVRPVEEYRAGHIPDALSVP
                     VDTLEAALQTLPRDKEVVAYCRGPYCVFSDEVVRALSARGYRASRLADGFPEWRAAG"
BASE COUNT         1053 a         2158 c         2063 g         1179 t
ORIGIN      
        1 cccgccgcct cttctccgac ccgctctacg ccgtcgtcgg cgagagcacg gggccatacg
       61 cggggggacg agaagcgccc cgaacctaat ctacggcagg atcaccctga acgacagcct
      121 cgtggggttg tgagacgggg catcatggca acaggtgcac gcagcttgaa tgagatgccg
      181 caactccgcg cagggtgccc gcatgcgccg gcactacctg gatatctgct ccttcgctca
      241 cgggccagga ctgacgccga cgttcaggga gaactcagaa gtgtcgccga tcgacgtgcc
      301 gctacccgtc ccactcgtgt tagtcgccgt cgcggtcaca gcttgtccga gttgcggaac
      361 agggctggcg caggagaagc tgtcggtccc gtcggcgtcc ccaccggcat cggtctggat
      421 cgtatcctgt cctaccggga tctggccctc gccgtggcct gaggggtcag gcacagccac
      481 gaagcactgg atggtgtagg tctggttggg gttgctgttt agcgtgccgg tgatggtcgt
      541 gaagaagggg gaggtggagc tctttatggc cgaggctatg acggggaagt tctggaggtt
      601 gttggctccg gtatccgggt catcggtgtc gttggctgtg acgccgtcgg ggaaaaggtt
      661 catcccgagg ccggtattcg agaagatgaa gttggagagg acgctgttgc ccctcccctc
      721 aacgatcgag acgccatcgt ccccgttgcc ggcgatggcg tttcccgctc cgctggtcgt
      781 gcccccgacg gtgttattgg acgcaccagc agtgatgatc acaccgtcac cgtcgttgcc
      841 aagatcaccg ctcccgtcgg ctttggtgcc tatgcggttt ccctccacct tgttgcccgt
      901 cgccccggaa ccactgatga agacgccaaa gcggttgccg gagatgacgt tgcgtgcccc
      961 actggccgtc cccccgacgg tgttgttggg cgcgatatcg atgaccacgc cgtcaccgct
     1021 gttgcccagg tcttgggtgc ctgaggcgct ggtgcctatg aagttgccct gtaccttgtt
     1081 gcctgtcgcc aaagagccag cgatctggac gccatcagcc acgttgctgg agaggacgtt
     1141 gcgctgggcg ggttgggtgc ctcccaccgt gttgttgggc gcgttagaga tgtccacacc
     1201 ggcgttgccg ttggccaggg ctccggttcc ggcggcattg gtgcctatga agctgccctc
     1261 tatcttgttg cccgtagccc cggagccagc gatctggacg ccgttgtcga agaagctgtt
     1321 tatcaccagg cccttgatag tgctgttcga ggcggtgatc ttgagcccat tcgcgccgaa
     1381 gcccgcgtcg gtcccgttca actgtatctt gagtaccgcg tcgttgcccg tggctagcgt
     1441 gttctcgctc gcccccggct gggtgtagcc gtttatcgtt acggggccgg ttatgtcagg
     1501 aagcgccctt aacccggaac tacccacgtt gatggtcttc acagaggctg tgccgcctat
     1561 attgaacttg atcgtatccg cccccgaggt gtcgtttgac tcctgaatcg ctgcccgcaa
     1621 ggtgcactgc ttgcccttgt tgcgcgaggt gtcgcacacg ctgtcggaga tccttctgtc
     1681 ttttgcgtca ctggacttgt tgacggtgaa ggtggtcgca gcatcggcca cgccggataa
     1741 gagcgcaagg aggagcaccc ccaaaagcgc gagcgccggc atagccgtca gcctgacaat
     1801 cgccattcgt ttcatctcac gctccacatc gtgggttgca tcccaggata gagcacggcc
     1861 cttagcccac acttagcccg aaagcccagt agtgtaggca taaatgccta gaagcacggc
     1921 gctccgcgcc tttcagcacg ccgtcttcga cggcttgtca gcttcagagg cgcggtccgt
     1981 tgctcccgcg cgtcgagcga accgggtaag tcctgaacag cccgaagctg aaatgccgaa
     2041 agcgaggccg ccggccgaag cgtgctgaca agcggaggcg ttagccggag cgggctgaaa
     2101 gcgaagcccg aagggcgtag cgtgctgaaa tgctatgaac cgcgttgctc gatgacccag
     2161 gcggcgatct gggctctgga gcccagactc agcttccgca gtatgcgccg gacgtgcgcg
     2221 tgtaccgtgc gttcagagat accgagttcg gtagcgacct ggcggttcgt caggcccttt
     2281 gccacttctt ccgccacctc ccgctcgcgg ggggtgagga cctccgcgtg cccggcggtc
     2341 ggccgttcgg ggatgcggaa ggctccctct tccgcgaggg cgtactcgat ggcctcttcg
     2401 gggctcatcg ctctcccctc ggcccacact ctctcccacg cgtcctcttc gatgagagcg
     2461 cgtgcggcgg ccaggcgctc ctcgtagcgg tagtgtgagc ggtcgaaggg cgtgaggtgc
     2521 atgcctatgg cctctctgag ggcctccgcg gcgccccaca ggcgcgccgc gcgatccggc
     2581 cgcgcccgct ctccagccac ccccgccata cccaacaggc agtaagctat gcccatcttg
     2641 tggcccattc cacgcagcag ttcgaggttc tccgcgaggt aggcctgcgc ctgctcgggg
     2701 ttgcccgccg tcaggctgac catgccgagg gcggcgaggg cgacgacggt gccctggacg
     2761 tccccgagcg cccggtacgc ggcgtaggcc tcggcggcgt agtccaacgc cccttcgtag
     2821 tcgaacgcgt ccagcgccgc cagcgttaga gcttcgagca gacccgcgcg cgaacgctgg
     2881 tccaggggac cccggaccaa cgcctcggcc tcttcccgca actgcgccac gcggacgctg
     2941 tcgcccgcgt gcgcgagcgc gtacaccagg ctgcgaaccg cgcccgcggc gctcgagctg
     3001 tctttcagtt ccctggaaag ggcgaggctc tcttcgagca tggcgatcgc ctgctcgaat
     3061 cctcctccga acagaataag aaaaccggtc tcgttgagcg ccctcgcgcg caagggcgtc
     3121 cgcagcgccc tgctcctgga gagcgccttc tccagccacc ggcgaccctc gctcacgccg
     3181 aagacgcccc agaaacgcac gagcgcgacc gagatccgca accctgcctc cgcccggtcc
     3241 tccgcgtcca gcgcccacga gagagccgcc cgtaggttgt cgcgttcctc cgacaaccgc
     3301 tccaaccacg gtcccggtcg cggtcctagc agcccgggct cggcctcctc cgcgagcgtg
     3361 agaaagtaca tcgcgtgacg ctcccgcgcc cgctcgtgcc gggcggcatc caaccgctcc
     3421 ctcgcgtact gccggactgg ttcgagcatc ccgtaccgcg ccgtgtccag actccctgtg
     3481 tgtagggtca ccaacgactt gctcaccagc ctgccgagaa ggtccagtac gtcagcgctc
     3541 tctatcccgt cacctatccc cacggcctca gcggcctcca gcgtccatcc gccggcgaac
     3601 accgagagcc gttcgaacag cctgcgttcc cggtcgctca acggctcgta gctccactcc
     3661 agagccgctc gcagggtctg gtggcgaggg tccgccgtcc ggcttccgcc ggtgagcagc
     3721 tccagggcgt cgtccagccg gactgcgacc tgctctatcg ccagcaccgt catccgcgcc
     3781 gtcgccagct ctatcgccag cgggaggccg tcgagcttgc ggcagatctt cgcaaccgcc
     3841 ccggagttcg cggacgtcaa ctcgaagccc ggaagccggg ttcgcgcccg gtccacaaac
     3901 agccgcaccg actcgtagcc ggcgaggtgt tcgacggtgg gccgatagtt aacgtcgggc
     3961 atcgagagcg aacgtaccgg ccagcagact tcgccggtga tccctagcgg ctcccggctg
     4021 gttgccagca cgcgcaggcc ggcacatgag gaaagtagcg tgtccgccag ccgggcgcag
     4081 gcgcccacga gatgctcgca gttgtccagg actagcaaga gttcgctgga tctcagttcc
     4141 tccaccaggg tagtgaagag cggacggccc tgctcttcgc ggacccccag cgcggcggcc
     4201 actgcctgcg gcgcgagatc agggtccgag agcggcgcga actccaccag ccaaacgccc
     4261 gccgggtaga cggtcgtcag gtcccccgcg acctccagtg ccagcctcgt cttccccacg
     4321 ccgcccgtcc cagtgagggt gaggagccgg gtcatgctca ggtggcgttt gatctccagg
     4381 atctccgctt ccctgccgat gaaattgctc acggcagccg gcaggttgtg ccgacggtcg
     4441 tcgaatgctt ttccggagcg ggaggccgtg cggtcggagg ctgttgcgcc aggatacgac
     4501 ggtagagacg cctggtggca ttatccggcg tcctgtcgag ctgatgccgg aaggtctctt
     4561 tcaaccgctc gtactggatt atggccttga cgggatgtcc ggcggccgcg tacaggcgca
     4621 tcaagccggt gtgggctttt tcgtggatcg gttgttccgt caccacccgc tcgaaggaca
     4681 gtatggcccg ttcgcgctca ccacgctctt cgtacgctcg ggccagctcg acgagtagcg
     4741 cgagatacaa ctgccgcaac tcttcccgcc tctcctccgt ccagaccccg tagcggtcct
     4801 cgggcagcag gtccccggcg tacaggtcca gcgccgctcg atacgcggcg ggctcccgct
     4861 ctcgccgagc ggttgccgcg gcctcctcga aggcttccac gtcaacccac agagggctct
     4921 caggacagag ctggagacgg ccgccgcgca ggcgcaggta tccagcaggg gcgcgagggc
     4981 ctggatccag gaccccgcga gccacgtgca gagcgtaatg gaggttgttg gccgcggcct
     5041 tcgccccgag gtcgggccag agcaggtcca tcgcctgctc ccggtgcaga tggtggcccg
     5101 gagccagcgc gagcagcttg accacgctcg cggccttgcg caaccgccac tcgttctcgc
     5161 caacgacccg cgacccgacc cggacctgga accctcctag taaacctatc cgaaggggtt
     5221 cgaggtccgc cgcgggagca tccccgttgc cacgcaccag cggagccgcc gaaccgggcg
     5281 ccgcctctcg gaaggacgtc atgagatcct ccttccggcc ctgctccgat gcctccacgt
     5341 accggaccca gatgaactct caggtaagat cgtgacaact cccccgctag agaaggacgc
     5401 ttatcgagga tctccgagag atggtgtcag catcggcggg gccggatgag agcgcgaagc
     5461 gaatccccca agggccagga gcgcaagcag cgccctccat ttcgggcttc ttatggccgc
     5521 tggccgagtc atagcctcga tcatctttcc cctgattgag gcttctcccc atttctcgcc
     5581 ccgtctctca tgtaatttat gcagcttgtg cctgccttat agcagttcgg gagaggtatc
     5641 gcgtccccca aattgcgtat ctttcgctag ctcaggacgc ctccggcgtt cgagcgttgg
     5701 gcgcccccag gcgtgcgcac gtggttagag ctacatcagc gccaccccgg aaccatcgtg
     5761 tcttgacgtc acgcgactat tcaattaatc tattgaatat atgaatactg ccgtgcacag
     5821 ggagttcaag gaccggctgt tcgagcaatt cgccaggatc ggcaaggctc tggcgagtcc
     5881 caagcgtttg gagatagtgg acctcctggc gcaaggcgag cgcacggtcg aggagatctc
     5941 gcgggagacg gcgatgccgg tggccaatgc gtcgcagcac ctccaggcgc tcaagggcgt
     6001 gcggatggtc gagtctcgcc gtgaggggct ctacgcatac taccggctgg cggacgaagg
     6061 tgtgttcctg acgtggcagg ccgtccgcgc cctcggggag acgcgcctgg ccgaggtcga
     6121 cagggtggtg gagacctacc ttgaggacag aggggctctg gaggccgtgg aggcgacgga
     6181 attgatggag cgactgggcg acgggagcgc ggttgtcctc gacgtacgcc cggtggagga
     6241 gtaccgggcc gggcacatcc ccgacgcgct ctccgtgccc gttgacaccc tcgaagcggc
     6301 cttgcagacg ctacccagag acaaggaggt agtcgcctac tgccggggac cctattgcgt
     6361 attctccgac gaagtggtgc gtgctctgag tgcccgagga taccgggcga gcaggctcgc
     6421 tgatgggttc cccgagtggc gggcggcggg ata
//
LOCUS       DATAIR010000134         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_423104, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000134 DATAIR010000000
VERSION     DATAIR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423104"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VHM69_03350"
     CDS             <1..533
                     /locus_tag="VHM69_03350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX2739459.1"
                     /translation="WNAKRMCTISRSKRPEGDAPVKNPPALARLEIRYGDLDPYGHVN
                     NAVYLEFFEKVRICYWRALADLAGIEKLEAGDVPGARYVIAETTVRFKTPIFFDDILH
                     GSARVRTVGNRSYTMDFELRVGETFESGTLAAEGSAAHVFFDPLENAVHPRPDWFLPT
                     VAKLEGRPEESFSPEA"
     gene            740..1270
                     /locus_tag="VHM69_03355"
     CDS             740..1270
                     /locus_tag="VHM69_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2739460.1"
                     /translation="MMRMLARVAALSLMMLVACAPAAWAQGEEVTVKMEDNFFSPANI
                     TVEPGTTVTWVQEGNNPHTTTSYDGLWDSGMIQPGSGGSFSFTFDEPGTFDYYCIPHE
                     SMGMIGSVTVSDASASASASGSASASASGSASASASESAPALTGTGGPSLALAAALLL
                     VGSGLASFAVLRRRAS"
     gene            1299..1490
                     /locus_tag="VHM69_03360"
     CDS             1299..1490
                     /locus_tag="VHM69_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739461.1"
                     /translation="MPLPCYTVFSTYSGVDSLCIGRLARARGPEGSQVCARQEGHFLE
                     RVADAPVTGRTLLAHLASG"
     gene            1622..>1920
                     /locus_tag="VHM69_03365"
     CDS             1622..>1920
                     /locus_tag="VHM69_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2739462.1"
                     /translation="MEYQTIKDEKVPSLGLGTYRLTGETCVRAVERALSMGYRHVDTA
                     QMYDNESEVGRGIQEAGIDRAEIFLTTKVWPSDFAHDRVIRKSKESLKKLRTGYV"
BASE COUNT          368 a          600 c          611 g          341 t
ORIGIN      
        1 gatggaacgc aaagagaatg tgtacaatct cccgcagcaa acgaccagaa ggggatgcgc
       61 ccgtgaagaa cccgcccgct ctcgcgcggc tcgagatccg gtacggggac cttgacccct
      121 acggccacgt aaataacgcc gtctacctgg agttcttcga gaaggtccga atctgctact
      181 ggcgcgccct ggccgacctc gccggcatcg agaagctgga agcaggcgac gtgcctggtg
      241 cccgctatgt catcgccgag acgaccgtcc gcttcaagac cccgatcttc ttcgatgaca
      301 tcctccacgg ctcagcgcgg gtacgcacgg ttggcaaccg ctcctatacc atggacttcg
      361 agctgcgcgt gggcgagact ttcgagagcg gcacgctcgc cgccgaaggc tccgcagccc
      421 acgtcttctt cgaccccctg gagaacgccg tccatccgcg ccctgactgg tttctcccca
      481 ccgttgcgaa gctcgaaggc cgtcccgagg agtctttctc ccccgaggcc tgatgccttc
      541 ggcgtccctg gcgccaccgt attcgccgag ccaggaggcc gacttcgcgc agctcggtat
      601 tcgggaacat ggagcaacaa aaagcacaag atcgcaaacc gaagggccgg agtcacgcgt
      661 ataaagcagc gtacggcggt gaagtcaggt gggcaacttc ccgtccgggg tgtcgtccgc
      721 gacgataagg aggagccata tgatgcggat gctagcgcgg gtggccgcgc tctccctcat
      781 gatgctcgtt gcctgcgcac cagcggcatg ggcgcagggc gaggaggtca cggtgaagat
      841 ggaggacaac ttcttctccc cggccaacat cacggtcgaa cccggcacca cggtgacgtg
      901 ggtccaggaa ggcaacaacc cgcacaccac gacctcctac gacgggttat gggactcggg
      961 aatgatccag ccgggctccg gtggatcgtt ctctttcacc ttcgatgagc ctgggacgtt
     1021 cgattattac tgcataccgc acgagtccat gggtatgata ggcagcgtca ccgtgagcga
     1081 cgcatcggca agcgcctccg cgagcggttc ggcaagcgcc tctgcgagcg gctccgcgag
     1141 tgcttcggca agcgagagtg cgcctgccct gacggggacc ggtggtccat cgctcgcgct
     1201 cgctgccgcc ctcttgcttg tgggatccgg tctcgcctcg ttcgctgtgc tgcggcgacg
     1261 ggcttcgtag gcatttatcc ggagaggagg ggtatcccat gcccctcccc tgttacacgg
     1321 tcttctccac gtactccgga gtagactctc tctgcatcgg caggctggcc cgcgcccgtg
     1381 gtcccgaagg gagtcaggtc tgcgccaggc aagaaggaca ctttctggag cgtgtcgcgg
     1441 atgcccccgt cacgggccgg acgcttctcg cacaccttgc ttccgggtga agcgtcggag
     1501 cattacctat cccgttcatc gaaaaacgac ggcgaaggct gtccggaagg ttcctccaca
     1561 cgggcacgac gtgttacggt tcacgggtcc ggttggcgtt ccgtcgtagg aggaggccgt
     1621 gatggagtac cagacgataa aggacgagaa ggttccttcc ctggggctgg gtacctacag
     1681 gctcacaggg gagacgtgcg tgcgggccgt ggagcgcgcc ctctcgatgg gatacaggca
     1741 cgtcgatacg gcccagatgt acgacaatga aagtgaggtc ggaaggggca tccaggaggc
     1801 ggggatagac cgggcggaga tcttcctgac aacgaaggtc tggccgagcg acttcgccca
     1861 cgaccgtgtg atccggaagt ccaaagagag cctgaagaag ctacgcacag gttacgtcga
//
LOCUS       DATAIR010000135         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_49796, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000135 DATAIR010000000
VERSION     DATAIR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49796"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            835..1506
                     /locus_tag="VHM69_03370"
     CDS             835..1506
                     /locus_tag="VHM69_03370"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2739463.1"
                     /translation="MRFKNLILILAALTVVFALAACSSAGQSSGDGGKKASEEQAQKQ
                     EPPKKMTEEAKNKQVAPKKGAEGGPGNGETKLEPPKSTELKLTVPKMKEIKNDTIPTG
                     IGTNEALFHDYAGVHLRRTGFPWHKTANVYIAGHRIGFPGTNSDLAFYDLEDLRKGDK
                     VYVKDADGRQYTYEVFKKLVVEPTDLSVLKPIKGKNILSLQTCTLPDYTDRVIYQAEL
                     KDIKS"
     gene            1696..2571
                     /locus_tag="VHM69_03375"
     CDS             1696..2571
                     /locus_tag="VHM69_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2739464.1"
                     /translation="MAAHSRDGGPVFGAHAFIRAGEWSREGAGCVISSAAEAGLDFVE
                     IPLLDPGSIDVEHTRSLLRHHDLGCTCSLGLPPDCHMPSNPEGAGRFLDGAVETAASL
                     DSPILTGPLYAHVGTLTGRPPQPEELETVARVLKETARRAAGHGVEIGIEAINRYETY
                     LVNLASQASELLDRIDEPNVFVHLDTYHMNIEEKGFYGPITGLGSRLGYIHLSESDRG
                     TPGTANVNWDEVFRGLKDIGYRGALAMESFAAVNEALMGATALWRDVVGDSDIFVRDG
                     LAFLHAKAEEHGVLG"
     gene            2604..>3878
                     /locus_tag="VHM69_03380"
     CDS             2604..>3878
                     /locus_tag="VHM69_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2739465.1"
                     /translation="MLLGLDLGTSSVKALLMEESGAAIGEGSAPYSVHSARPGWAESS
                     PDDWWAAVLRATGAAVGGRGAEVKALGLSGQMHGVVLADERGRPLRPAVLWADARSGG
                     QLSAYLRLDESLRHRLANPPAAGMAGPTLLWLRDNEPGDYAGARWALQPKDWLRMLLT
                     GEAAAEPSDASATLLYDLIADDWSHAAVDDLGLRAELLAPLVPSGGVAGTLARDAAGA
                     LGLREELPVAAGAADTAAAMIGTGLLHPGPVQLTVGTGGQIVTPKGAPHPDPHGRTHL
                     YRAAVPGLWFSMAAIQNAGLALEWVRKILGVSWEQVYEEAFAVPPGSGGVVFLPYMSG
                     ERTPRFDPGARGAWTGLGLDNTRGHLLRAALEGVAFALREGLEALEDLGINAPELRLA
                     GGGTGGSSGGPWRQLLADVLGRPLRLLPDEVSA"
BASE COUNT          739 a         1213 c         1259 g          667 t
ORIGIN      
        1 tgacccaaca atagcgggcg gcaatgaatc gacggtgaat caccagggat gaggtgactc
       61 accttcggag aatctactta accccgaggt tgaatagctc atagccccgg acggccaagg
      121 acggccggcg gggttagggg ccgggtacag cgaaggccgc tctacggggc tgcggctgtg
      181 tccttcctac acgtaattca gcacgagaaa tgcaagggcg gctcccacag cgatgccgag
      241 ggcgagccct gcgattagtc tcgcgacgcg tcggcggtcc acctgaactc tcctctcagc
      301 cgggggctgg gacacccgat caggataccc cggcccgcca ggtacgtgac actactactt
      361 gcgagggccg acgtcttccc tgtagcgcct gatctgcttt ccggccttcc tccacgattg
      421 cccgttcact gtggccgggt ataagcatgt gtggtgagtg gggcgtcaca ttgcctcaaa
      481 gctactttca ggcgcttgtt ggtcgcgctc cagctcctcc ctccgggtcc ttggaagcca
      541 tgacttctca agaacgcaac caactctaac ggatgctgcg tattaacgag agagtgcacg
      601 tagatggcgc aatcgacatc ggagggtcgc caagagtttg caattcggat agtattcctt
      661 aatatatcgt ccgacaaggt agatccaggc gtgacgtggg caagtccgtg gcgcgggagg
      721 tctcttgcgg tgaggcttgt aacggagttt tgatccaaat gttaccgcct tgttattttt
      781 gacctgtatg ctttcaaagt acggcgaccg ggtaagacag cgagaggtga tgagatgcgt
      841 ttcaagaacc tgatattgat actggcggca ctgacggtgg tgtttgcgct ggcggcctgc
      901 tcttccgcgg gccagtcctc aggagatggg ggaaagaaag cttcagagga acaggcgcag
      961 aagcaggaac cgccgaagaa gatgacggag gaggccaaga acaagcaggt cgccccgaag
     1021 aagggtgccg agggaggccc cggcaacgga gagaccaagc tcgagccccc caagagcaca
     1081 gaactcaagc tcacggttcc gaagatgaag gaaatcaaga acgacaccat cccgaccggt
     1141 atcggcacca acgaggcgct gttccacgac tatgccggcg tccatctcag gcggacgggc
     1201 tttccgtggc ataagacggc caacgtgtac atcgccggac accgcatcgg gttcccgggg
     1261 acgaacagcg acctggcttt ctacgacctc gaagacctca ggaagggcga caaggtctac
     1321 gtgaaggacg ctgacggcag gcagtacaca tacgaggtct tcaagaagct agtagtcgag
     1381 cccaccgacc tctccgtgct gaagcccatc aagggcaaga acatactcag cctgcagacc
     1441 tgcacgctgc ccgactacac agaccgcgtc atctaccagg ccgagctcaa ggacatcaag
     1501 tcgtaagcta gagacaacca cagagacgag tacagggccg gagcaacgct ccggccctct
     1561 tgttttgtgt gaggtgcgtt gattacaaag aagcgatgaa cctacaataa gagccgcggc
     1621 gcgcgacctg ccggtaacgt tcggggagac tcgcttcgaa gcccgtgcgc ccggggaaca
     1681 gaaagtggag gagagatggc tgcgcactca agggacggcg gtccggtctt cggggcacac
     1741 gcctttatcc gggctggaga gtggtcgcgc gagggcgccg ggtgtgtgat ctcctccgcc
     1801 gccgaggctg gcctggattt cgtcgagata ccactcctgg atccaggctc gatagacgtc
     1861 gaacatacac gttctctgct gcgtcaccac gatctcggct gtacctgctc cctcggcctg
     1921 ccgccggatt gccacatgcc atccaaccca gaaggcgcgg gacgcttcct cgacggggcg
     1981 gtggagacgg ccgcctctct ggactcaccc atcctcaccg gtcctctgta tgcgcacgtt
     2041 ggcacgctga cgggcaggcc gccgcagccg gaagaactag agaccgtcgc ccgcgtcctc
     2101 aaggagacgg cccggcgggc cgccgggcac ggcgtcgaga tcgggatcga ggccatcaac
     2161 cgctacgaga cgtacctcgt caacctggct tcgcaggcct cggaactgct tgaccggata
     2221 gacgagccga acgtcttcgt ccacctcgac acgtaccaca tgaacatcga agagaagggc
     2281 ttctacgggc cgatcaccgg cctcgggtct cgtctgggat acatccacct ctccgaatcg
     2341 gaccgcggca cgccggggac ggcgaacgtc aactgggacg aggtcttccg cggcctgaag
     2401 gacatcggct acagaggtgc actcgcgatg gaatccttcg ccgccgtgaa cgaggcgctc
     2461 atgggtgcga cggcgctctg gcgcgacgtg gtcggggatt ccgacatctt cgtcagggat
     2521 ggcctcgctt tcctgcacgc gaaggcagaa gagcacgggg tgctcggatg aataaccatc
     2581 cggtgtcggc gtaaactttc tctatgctcc tcggcctcga cctcgggacg agttctgtga
     2641 aagcgcttct catggaggaa agcggcgccg ctatcggtga ggggtccgcg ccctattctg
     2701 tccactcggc acgtccaggg tgggccgagt cttctccgga cgactggtgg gcagcagtcc
     2761 tgagggcgac gggcgccgcc gtgggcgggc gtggcgcgga ggtgaaggcg ctcgggctct
     2821 cgggacagat gcacggcgtg gtcctcgcgg acgagcgggg gcgtcccctg agacccgccg
     2881 tcctctgggc cgacgcccgg tcaggaggac agttatcagc ctacctcagg ctggatgaga
     2941 gcctgcgtca tcgtctcgcc aacccgccgg ccgccggcat ggcgggcccg accctcctgt
     3001 ggctgcgcga caatgaacct ggtgactacg cgggtgccag gtgggcactg cagccgaagg
     3061 actggctcag gatgctactc actggggagg ccgccgcgga accctccgac gcctcggcca
     3121 ccctgctcta cgacctcatc gccgacgact ggtcccacgc ggctgtcgat gacctcggcc
     3181 tgcgagcgga gctgctcgcc ccgctggtcc cctcaggggg cgtcgcaggg acgctagcca
     3241 gagacgctgc aggcgccctc gggttgcggg aggagttgcc tgtcgcggca ggagcagccg
     3301 ataccgctgc cgccatgatc ggcaccgggc ttctgcatcc cggtcccgtg cagctcaccg
     3361 tcggcacagg agggcagatc gttacgccga agggagctcc gcatccagac ccgcatggtc
     3421 gcacccacct ctaccgcgcc gcggtccccg gactctggtt ctcgatggcg gcgatccaga
     3481 acgcgggtct cgccctggag tgggtacgca aaatactcgg cgtctcctgg gaacaggtgt
     3541 acgaggaagc tttcgccgtc ccgccgggct ctggaggcgt ggtgtttcta ccgtacatga
     3601 gcggggagag gacgccgcgc ttcgatcccg gcgcacgtgg cgcgtggacc ggcctcggcc
     3661 tcgacaacac gcgcggtcac ctcctgcgcg cggccctcga aggcgtcgct ttcgccctgc
     3721 gcgagggcct cgaagcgctt gaggacctgg ggatcaatgc tcccgagctc cgcctcgccg
     3781 gtggtggaac cggaggaagc tcgggcgggc cctggcggca actgctggct gacgtgctcg
     3841 gccgcccgtt gcggctcttg cccgatgagg tctccgcc
//
LOCUS       DATAIR010000136         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_8067, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000136 DATAIR010000000
VERSION     DATAIR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8067"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VHM69_03385"
     CDS             complement(<1..1799)
                     /locus_tag="VHM69_03385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2739466.1"
                     /translation="MSRRAVESLGDTVASLPPREATLVKLPDLHPELSEALHKMGASR
                     LYSHQLEAYERVRAGENVVVATATASGKSLCYKIPAFQNALDRANRRAVFLYPTKALA
                     QDQLGKIKAFGLRGVHPATYDGDTPHALRADIRRRSNILLTNPDMLNIGILPNHEAWA
                     DFLRNLEIVAVDEAHVLRGVFGSHVAAVLRRLRRVAMLHGGDPQFVLTSATIANPQEL
                     AESLTGLPFALVDNDGASTGERRVVFRNPPLLDKEKGERRSLLTEGALVFANLVSQGV
                     RTIAFAKTRKSAELIYRYAADRLGVDGARRISPYRAGYTPRERREIESRLFRGDLLGV
                     VSTNALELGVDVGALDAVVCCGYPGSVASIWQQWGRAGRGKDPALAVYIAGRDSLDQF
                     LFENPPRVLGRRVEAARVTMENPYILGPHLLAAANEAPLDADDEEYFGPAYRSVAKTM
                     MEARLLASSGGRLVYVGGDNPARNISLRSASSDTVLIADGEGELIGTAEATRAPSELH
                     PGATYLHRGTAYEVDELDLRTHRAVARRVPNRYYTRPRVETDVEVLEEIENRDLANGA
                     VLHWGRVRTTDSVTYFKKVRVADDKEIGVYPLDL"
     gene            complement(1834..2433)
                     /gene="recR"
                     /locus_tag="VHM69_03390"
     CDS             complement(1834..2433)
                     /gene="recR"
                     /locus_tag="VHM69_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX2739467.1"
                     /translation="MATYARPVERLITELSKLPSIGPRSAQRIAFHVIRGKKEDAIGL
                     AEALQEVKERIKPCKRCFNLTEADECEICRDARRDRSVICVVEDPYDIGPMERTHEFR
                     GLYHVLGGALSPLDGVEPEDLRIAELIERVRNEGTSELVIATNPNTTGEATALFIAQE
                     VKDLPVRVTALASGLPVGGDLEYADEVTLGRAFAARREL"
BASE COUNT          379 a          795 c          819 g          453 t
ORIGIN      
        1 agatccaggg ggtagacccc gatctccttg tcgtcagcca ccctgacctt cttgaagtag
       61 gtgacggagt ccgtcgtcct taccctgccc cagtggagta cggccccgtt cgcaaggtca
      121 cgattctcga tctcctcgag aacctccacg tccgtctcga cccgtggcct cgtgtagtag
      181 cggttcggga cgcggcgggc gacggccctg tgggtgcgga ggtccaactc gtccacctcg
      241 tatgccgtcc cgcggtggag gtacgtcgcg cccggatgca gctcgctcgg ggcgcgggtg
      301 gcctcggcgg tcccgatgag ctctccttca ccatccgcta tgaggaccgt gtcggacgac
      361 gccgaacgca atgagatgtt gcgcgccggg ttatccccgc caacgtagac gagccgtcca
      421 ccgctcgaag cgagcaacct ggcctccatc atcgtcttcg ccacgctcct gtaggcgggc
      481 ccgaagtact cctcgtcgtc ggcgtcgagc ggggcctcgt tggccgcggc gaggaggtgc
      541 ggcccaagga tgtaggggtt ctccatcgtc accctggcgg cctccacgcg gcgtccgagg
      601 acccgtggcg ggttctcgaa gaggaactgg tccagcgagt ctcgtcctgc gatgtagacg
      661 gcgagggcgg ggtccttgcc acgtcctgcg cgtccccact gttgccagat ggaggcgacg
      721 ctgcccgggt atccgcagca gacaacggcg tccagggcgc ccacgtccac cccgagctcc
      781 agggcgttcg tcgagacgac gccgaggagg tcgccgcgga agaggcggct ttcgatctcc
      841 cttctttcac gcggggtgta tcccgcacgg taaggggaga tccttcgcgc cccgtcaacg
      901 ccgagcctgt cagcagcgta gcggtagatg agctccgccg acttcctggt cttggcgaaa
      961 gcaatggttc tcaccccttg tgagacgagg ttcgcgaaga cgagagcccc ctctgtgaga
     1021 aggctccgcc tctccccctt ctccttgtcc agcaggggag ggttgcggaa gacgacgcgc
     1081 cgctcgcccg ttgaggcacc gtcgttgtca acgagggcga aagggaggcc agtgaggctc
     1141 tctgcgagct cctgcgggtt ggctatcgtg gcgctcgtaa ggacgaactg cgggtcgccg
     1201 ccgtgcagca tcgccacgcg tctgagacgg cgcagtacgg cggcgacgtg cgagccgaag
     1261 acgccgcgca ggacgtgcgc ctcgtcgacg gccacgatct ccaggttgcg gaggaaatct
     1321 gcccatgctt cgtggttggg cagtatgccg atgttcagca tgtcagggtt ggtaaggagg
     1381 atgttcgacc ggcgccggat gtcggcgcgg agtgcatgcg gtgtgtctcc gtcgtaggta
     1441 gccggatgga cgccgcgcag accgaaggcc tttatcttgc ctagctgatc ctgggccagc
     1501 gccttcgtag gatagaggaa gaccgcacgc cgattcgccc tgtccagcgc gttctggaag
     1561 gccggtatct tgtagcagag cgacttcccg ctcgccgtcg ccgtcgccac gacaacgttc
     1621 tccccggccc gtacccgctc gtaagcctcc aactgatgcg agtacagcct ggaggcgccc
     1681 atcttgtgca gggcctcgga aagctcgggg tggagatcgg ggagcttcac gagcgtcgcc
     1741 tcacgcggcg ggaggctcgc aacggtatcc ccgagggact cgaccgcgcg gcggctcatc
     1801 cctgcgaggg gctttggccc ggtggtcctc gtcttagagc tcacgccttg cggcgaaggc
     1861 ccgtcccagg gtcacctcgt cggcgtactc gaggtctccg cccacgggga ggccgcttgc
     1921 gagcgccgtc acccgcacag gcaggtcctt cacctcctgc gcgataaaca gcgcggtcgc
     1981 ctcgcccgtg gtgttcgggt tggtggctat gaccagctcc gaagtgcctt cattacggac
     2041 gcgctcgatg agctcggcaa tccgcaggtc ttccggctcg acgccgtcca ggggagagag
     2101 ggcgccgccc aggacgtggt agaggccgcg gaactcgtga gttcgttcca tcggaccgat
     2161 atcgtatggg tcttcgacga cgcagatcac ggaccggtcg cggcgggcgt cgcggcagat
     2221 ctcacactcg tcggcttcgg tcaggttgaa gcagcgcttg cacggtttga tcctctcctt
     2281 gacctcttgc agcgcctcgg cgagcccgat ggcgtcctct ttcttccccc tgatgacgtg
     2341 gaaggcgatg cgctgggcgc tcctcggtcc gatggagggt agcttggaga gttcagtaat
     2401 gagccgttct acgggccgcg cgtacgtcgc caaagaaccc ctaaag
//
LOCUS       DATAIR010000137         5080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_423253, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000137 DATAIR010000000
VERSION     DATAIR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423253"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VHM69_03395"
     CDS             <1..237
                     /locus_tag="VHM69_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739468.1"
                     /translation="QVCAAFLGAGANISAGALGVGLGLLGYFLGSRRLATVTVFLCVA
                     AILFGLAASQGQVPGIAPSDHAIPSIFWANENAG"
     gene            221..505
                     /locus_tag="VHM69_03400"
     CDS             221..505
                     /locus_tag="VHM69_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739469.1"
                     /translation="MRTRVDRTATLVRPENATPGRRPGRRWSIAGIICGFVAVVLLPI
                     VLGPLGVILGIVGLVKGDRALGVVAIVVGIVGLVLGFVFSAFVISLTNQT"
     gene            541..1299
                     /locus_tag="VHM69_03405"
     CDS             541..1299
                     /locus_tag="VHM69_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX2739470.1"
                     /translation="MERVRLTPPGEKRARGGRARLYVRWMPHLGAEAVALYELLRILP
                     DLGNDEVEVEELAELLRSTTESVEASFETLTEAGFIAVREGWMEVAENPPSAGTKVRS
                     HETQTNKVPDRDVEVIRAAPEGVSPDDYFRYMGSLPAPHILEFLNGYCERDGLTPDAV
                     REALKIASERDARRVGYVRSILERWVERGVKTVEDVERFEQDRKLRLVEQSGAVKGGA
                     LRIREAREGVSGGSDRQSAARRREGYEWLFGETE"
     gene            1271..2095
                     /gene="zapE"
                     /locus_tag="VHM69_03410"
     CDS             1271..2095
                     /gene="zapE"
                     /locus_tag="VHM69_03410"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HEX2739471.1"
                     /translation="MSGSSGKRNRGEARVLRLDDQVCPHCGRELQAEWDEFPPALQKK
                     YGKPGEWYYHPCAPECEKKNERREWELMRRDARVAALRERSGLSKRMKGYALDNYDHQ
                     LSKSAEKAFARAMDYIMRWKENREEGRGLYFCGDVGTGKTHLAVAVMNELMTRKRVPS
                     LFVTVPELLDNMRGTYNNPGRDIDEWMDSVKNADLLLLDDLGAERANEWVRERIFVIV
                     NHRYREELPTIFTSNTGPKDLAAQLGERTASRIIAMCDWISLEGEDYREAEVRRGL"
     gene            2646..3326
                     /locus_tag="VHM69_03415"
     CDS             2646..3326
                     /locus_tag="VHM69_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739472.1"
                     /translation="MRDEFLITRSGKQYVLFAGLLDEAHSLGLRGIDTDLIQVPDESN
                     GNVAIVKAMAEMEDGRRFSGIGDASPENVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TALEELSEGDDAPPASSSRGFEGSSSRGKPTPIRGGRQNSSQGPSQSAPAEQDTPNGQ
                     ADQPAPSKRGGKARKSQVDLLKTLAVEWRGENGVERLESRLGKPLTDLTRAEADEWID
                     RLTPEGRE"
     gene            3430..3810
                     /locus_tag="VHM69_03420"
     CDS             3430..3810
                     /locus_tag="VHM69_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739473.1"
                     /translation="MNAGDDAHFGHPRDRFGGWVEVYCDRLDPDALLGSMKAGRYYST
                     QGPALREILVDGDRLRVETSGVYAITLTGGGDRWQSGSERHSETGEPIFEAEFDLDPF
                     RGSYCRVTVVDTAGRRAWSNPVWP"
     gene            complement(4161..>5080)
                     /locus_tag="VHM69_03425"
     CDS             complement(4161..>5080)
                     /locus_tag="VHM69_03425"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2739474.1"
                     /translation="TGGTMNETITMPGKTRELHNHHFDSTIWNDLRFRDDDVIISTYA
                     KSGTTWTQQIVAQLLIGPDPDLEVAEMSPWLDLRVPPKEVKLPMVEAQTHRRFLKTHL
                     PLDALVFSEKAKYIYIGRDGRDVVWSMYNHHVNANGLWYEALNDTPGRVGPPIEPPPG
                     DIRQYWREWMDRDGHPFWPFWENVRTWWEIRHLPNVLFVHFANLKRDMPGQMRRIAEF
                     LDVPIDETRWETILEYCSFDWMKEHATKSVPLGGAFWDAGAQVFINKGVNGRWTATLT
                     PEAVAEYETRAVQELGPEAARWLTTGEGP"
BASE COUNT          988 a         1533 c         1694 g          865 t
ORIGIN      
        1 caggtctgcg ccgctttcct cggcgcgggc gcgaacatct cggccggcgc gctcggcgtg
       61 gggctcgggc tgctcggcta cttcctgggc tcaagaaggc tcgcgaccgt taccgtcttc
      121 ctttgcgtcg ccgccatcct cttcgggctc gccgcgagcc agggccaggt cccgggtatt
      181 gcgccctcgg accacgccat tcccagtatc ttctgggcaa atgagaacgc gggttgaccg
      241 aaccgcgacc ttggtacgcc ccgaaaacgc cacgccgggc cgccgtcccg gcaggcgctg
      301 gtccatagcg ggaataatct gcggcttcgt tgccgtcgtc ctgctcccca tcgttctagg
      361 accgctcggc gtcatactcg gcatagtcgg gctggtgaag ggcgaccggg ctctcggcgt
      421 cgtcgcgata gtcgtcggca tagtgggcct cgtgctcggc ttcgtgttca gcgcgttcgt
      481 gatctccctg accaatcaga cgtagagagc ctcgcccatc gccgctatac tggcctcagg
      541 atggagagag ttcggcttac acctccgggg gagaagaggg cgcggggagg ccgcgcccgc
      601 ctctacgtgc gctggatgcc acaccttggg gccgaggcag tcgccctcta cgagctgctg
      661 cgcatcctcc ccgacctcgg taacgacgag gtcgaagtcg aggaactggc ggagctactg
      721 cggtctacca ccgagagcgt cgaagcgtct ttcgagaccc tgaccgaagc gggcttcatc
      781 gccgtgagag aaggctggat ggaggtcgcc gagaacccgc cttccgcagg gacgaaagtg
      841 agatcccacg aaacacagac aaacaaggtc cccgaccgtg acgtcgaggt gataagggct
      901 gcgcccgagg gagtttctcc cgacgattac ttccgctata tggggtcgtt gccggcgccg
      961 cacatccttg agttcctgaa cggctactgc gagagggacg ggctgacgcc cgacgctgtg
     1021 agggaggcat tgaagatcgc cagcgagcgt gacgcgcgca gagtcggcta cgtacggagc
     1081 atcctggagc gttgggtcga gcggggcgtg aagaccgtcg aggacgttga gcgcttcgag
     1141 caggatagaa agctgcgtct cgtcgagcag agcggggccg tgaagggcgg ggcgctgaga
     1201 ataagggaag ccagagaggg ggtgagtggt ggatcggatc gacagtctgc tgccagacgc
     1261 cgggagggct atgagtggct cttcggggaa acggaatagg ggcgaagcca gggtactgag
     1321 gctggacgat caggtctgcc cccactgcgg gcgcgagctg caggccgagt gggacgagtt
     1381 cccgcccgcc ctccagaaga agtacggcaa acccggcgag tggtactacc acccgtgcgc
     1441 gcccgaatgc gagaagaaga acgagcggcg cgagtgggag ctgatgcgcc gcgacgcgag
     1501 ggttgctgcc ctcagggagc gcagtggact gtccaagcgc atgaaaggct acgcgctcga
     1561 caactacgac caccagctca gcaagagcgc cgaaaaggcc ttcgccaggg ccatggacta
     1621 catcatgagg tggaaggaga acagggaaga gggtcggggg ctgtacttct gtggtgatgt
     1681 gggaacagga aagacgcacc tcgccgtcgc ggtgatgaac gagctcatga caaggaagag
     1741 ggtgccctcg ctcttcgtca cggtgcctga gcttctggac aacatgaggg ggacgtacaa
     1801 caaccctggt cgggacatcg acgagtggat ggactccgta aagaacgcag acctcttgct
     1861 gctcgacgac ctcggcgccg agagggcgaa cgagtgggtg cgcgagcgca tcttcgtcat
     1921 cgtcaaccat cgctacagag aggagctgcc gaccatcttc acctccaaca cggggcccaa
     1981 ggacctcgcc gcccagctag gagagaggac ggccagcagg atcatcgcga tgtgcgattg
     2041 gatctccctc gaaggcgaag attaccgcga ggccgaagtg aggcgtggcc tgtgagagac
     2101 gagttcatga tcacccgtca gggaaagctc tgcgagtgtg gatgcggcat gcctgcaccc
     2161 atagctacac gcaacaactc atcgaaggga tatgtcatcg gcgagccgat gcgttttgtg
     2221 cgtgggcatg caacacgcaa gaatgtcccc gatttcgtca tagatgcgaa tggttgctgg
     2281 atatggcagc gaggacttaa tccagtaagc ggctatgggc agatgagttc aggtgattat
     2341 gctcatcgtg taatgtatcg tgagtacaaa ggccagatac cagaaggcat gcagatcgat
     2401 catctgtgcc gagtccgagc ttgtatcaac ccagatcacc tcgaggttgt gaccagaagc
     2461 gagaacgcgc ggcgcggtct gactggacga caaccaaagt tagcaattcg acggctaact
     2521 gatgcccaga ttcgagaaat cagaatatcc ggacagtcta atcgtgcttt agcaagtcag
     2581 tacggtgtca gtcacgagac gattcgctgt gtgaggaacg gacgccacta tgcggagatc
     2641 gcgcaatgag ggacgaattt ctcattacta ggagtggcaa gcaatacgtc ctgtttgcgg
     2701 gcctcctcga cgaggcccat agcctcggcc tcaggggcat agacaccgac ctgatccagg
     2761 tccccgacga atcgaacggc aacgtagcca ttgtgaaggc gatggccgag atggaggacg
     2821 gccgccgctt ctcgggaata ggtgacgcga gccccgagaa cgtcgggcgc aacatcgccc
     2881 cgcacctgat ccggatggcc gagacccgtg cgaaagcccg tgccctgcgc gacgcggtga
     2941 acgtcggcgc gacagccctg gaagaactct cggagggcga cgacgccccg ccggcctctt
     3001 cttcccgcgg cttcgagggc tcgtcctcgc gcggcaagcc aacccccatc cgcgggggta
     3061 gacagaattc ttctcaaggc ccttcccaaa gcgctcccgc cgagcaggac accccgaacg
     3121 ggcaggctga tcagcctgcc ccgtcgaaga gaggcggtaa ggcgcgtaag agccaggtag
     3181 atctcctgaa gacgctggcc gtcgagtggc ggggcgagaa cggcgtcgag cgcctggaga
     3241 gccgcctcgg caagcctctc actgacctca cccgcgccga agccgacgag tggatcgacc
     3301 gcctcacccc ggaaggacgc gaataaccgc ttccaccgcg agggtccgat gcgcgaaccg
     3361 gcagcgtatc atcggagatc agacctacat acgtgggatg aagtcgaact ccagggccgc
     3421 agggtgctgg tgaacgcggg ggacgacgca cacttcggcc accctcgcga ccgcttcggg
     3481 ggctgggtgg aggtctactg cgaccggctc gacccggatg cccttctggg ctcgatgaag
     3541 gcggggcgtt actactcgac gcagggaccg gcattgcggg aaatactcgt ggacggcgac
     3601 cgcctgcgag tggagacgag cggcgtttac gcgatcacgc tgaccggggg cggtgaccgg
     3661 tggcagagcg ggtcggagcg ccatagcgag actggcgagc cgatcttcga ggcggagttc
     3721 gacctggacc cgttccgcgg ctcctactgc cgcgtcaccg tggtcgacac ggcgggtagg
     3781 cgtgcctgga gcaaccccgt gtggccgtga ggcgtgcaag aagcaggttg gcagagtgcg
     3841 aatcggcggc ttgagatcac ccacccggcg attatccgca tccaccacga gactgagcct
     3901 tctattttcg ccgggatgcg tagcgcgaac ccccactgaa ttcgaggaga aatacccgaa
     3961 agtcgaccga cctcggaatg ctgtggaagc agggcgtgcc gccagcttcg ctgccgcagc
     4021 attccccagc gacagccagg gctgacgcgc ctcatccggc ggaagtctca ggggaccccg
     4081 caccggcaga cgatcccgaa acgcccgcga ccagctcatc accagcgctc cgctcccgca
     4141 gacggtcagc caggcaagct ctacgggccc tctcccgtgg tgagccatcg cgcggcttcg
     4201 gggccgagct cctgcaccgc ccgcgtctcg tactcggcca cggcctccgg tgtgagggtc
     4261 gcggtccagc ggccgttcac tcctttgttg atgaagacct gcgcgccagc gtcccagaag
     4321 gcgcctccga gggggacgct cttggtggcg tgctctttca tccagtcaaa ggagcagtac
     4381 tcgaggatcg tctcccaccg cgtttcgtcg atgggaacgt cgaggaactc ggcgatgcgg
     4441 cgcatctgtc caggcatatc gcgcttgagg ttcgcgaagt gcacgaacag gacgttcggg
     4501 aggtgccgga tctcccacca ggtgcgaaca ttctcccaga acggccagaa ggggtgtccg
     4561 tcgcggtcca tccattcgcg ccagtactgc ctgatgtcgc ctggaggcgg ctctatcggc
     4621 ggcccgacgc gccccggcgt gtcgttgagc gcctcgtacc aaagcccatt cgcgttgacg
     4681 tggtggttgt acatgctcca cacgacgtcg cgcccgtcgc gcccgatgta gatgtacttt
     4741 gccttctctg agaagacgag ggcgtccagg gggaggtgcg tcttgaggaa ccgccggtgt
     4801 gtctgcgcct ccaccatcgg cagcttcacc tccttcgggg ggacgcgaag gtcgagccag
     4861 ggcgacatct cggccacctc gaggtcgggg tcggggccga tcagcaactg cgcgacgatc
     4921 tgctgcgtcc aggtggtgcc ggacttggcg taggtcgaga tgattacgtc gtcgtcgcgg
     4981 aagcggagat cgttccagat cgttgagtcg aagtggtggt tgtgcagctc ccgggtcttc
     5041 cctggcatgg tgatcgtctc gttcatggtt cctcctgttc
//
LOCUS       DATAIR010000138         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_423256, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000138 DATAIR010000000
VERSION     DATAIR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423256"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1207
                     /gene="sucC"
                     /locus_tag="VHM69_03430"
     CDS             56..1207
                     /gene="sucC"
                     /locus_tag="VHM69_03430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX2739475.1"
                     /translation="MDLYEYQGKELLRSYGLETLPGIVAGTPEEARRAAETLEGTVAI
                     KAQVLTGGRGKAGGIKVVNTPEEAEEAAEQILGMDISGHTVRRVYVEGGAEIDREFYL
                     SITVDRTAKRPLILFSTEGGVDIEEVAETKPEAIVKLHVDPLLGLLPYQVREVVFAAG
                     LEGDAAKGVGRTLTNLYKAFDGLDASLVEINPLVVTEDGRVLTLDAKVTVDNSSLYRH
                     PDIAELHDVEAADPQEQRAQEVGLQYVKLDGDVGILGNGAGLVMSTLDVVAQAGGEPA
                     NFCDVGGGADAEQIATALDIVTSNEQVESVLFNIFGGITRGDEVARGLLSAIERTGIT
                     LPIVVRLDGTNAEEGRQILAENTPENVVTEETMLDAARRAVELARDGGR"
     gene            1211..2086
                     /gene="sucD"
                     /locus_tag="VHM69_03435"
     CDS             1211..2086
                     /gene="sucD"
                     /locus_tag="VHM69_03435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2739476.1"
                     /translation="MAILVDEGTRLLVQGITGREGAFHTGRMLESGTKVVAGVTPGKG
                     GQDYEGVPIFNTVAEAVEETGANASVIFVPPPFAADAIFESLDAGLSTICCITEGIPV
                     HDMMRVADYLKMKDATLIGPNCPGVLSPGKSNVSIMPRDIFTPGNVGVVSRSGTLTYQ
                     IVNELTQRDIGQSTAVGIGGDPIIGTDFIEILSKFEADPETECAVMIGEIGGNAEQLA
                     AEYVQSEMSTPVVAYIAGFTAPEGKTMGHAGAIVSGGESTAEAKSEALRAAGIRAATN
                     PSEVAELVAEALGKG"
     gene            2222..>3182
                     /locus_tag="VHM69_03440"
     CDS             2222..>3182
                     /locus_tag="VHM69_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2739477.1"
                     /translation="MAEVSKERPARRFMRWLLRERIEEVQGPEAREGEEQHAWWQVVC
                     LTGVDYFSTLGYIPGIAALAAGSLSPIATLLIVLLTLFGMLPMYRVVAAESPHGQGSI
                     AMLERLLSFWQGKLFVLCLLGFVATSWIITITLSASDATAHVVENPLVPEAFRNHEVA
                     ITIILLAVLGAVFLKGFREAIGVAVAIVGVYLLVNIVVVVAGLYEIFTHPATFIGWQD
                     ALFTNYGNPLIMIGVSLLVFPQLALGLSGFETGVSMMPLVRGDPDDDHEQPEGRIRNT
                     RKMLFVAAIIMSFYLITTSFVTSVLISAQAFEEGASDRALAYLA"
BASE COUNT          628 a          990 c         1037 g          527 t
ORIGIN      
        1 gcgagtctct gtataatcac cggcgtcatg gagggcttcg cgggagtctt ggatcttgga
       61 tctctacgag tatcaaggta aggagctgtt gaggagctac ggtctggaga cgctgcccgg
      121 catcgtcgcg ggaacgccgg aggaggccag acgggcggcc gagacgcttg aagggaccgt
      181 ggcgataaaa gcccaggtcc tgacaggcgg gcgcggcaag gccggcggca tcaaagtagt
      241 caatactccg gaggaggccg aggaggcggc cgagcagatc ctcggcatgg acatcagcgg
      301 gcataccgta aggcgcgtct acgtcgaggg cggcgcggaa atagacaggg agttctacct
      361 ctcgatcacg gttgacagga ccgccaaacg gcccctgata ctgttctcca ccgagggcgg
      421 agtggatatc gaggaggtcg cggagacgaa acccgaggcc atcgtcaagc tgcacgtgga
      481 cccgctcttg gggctcctgc cataccaggt gcgcgaggtc gtcttcgccg cgggtctcga
      541 aggagatgcc gcgaagggag tcggaaggac gcttacgaac ctctacaaag ccttcgatgg
      601 gctcgatgcg agcctggtcg agatcaaccc tctcgtcgtt acggaggacg gtcgtgtact
      661 gacgctggat gcgaaggtca cggtggataa ttcgagcctg tacaggcacc ctgacatagc
      721 cgagctgcac gacgtagagg ccgcggaccc gcaggagcag cgggcgcagg aggtgggcct
      781 gcagtacgtc aagctcgacg gcgacgtcgg catcctcggc aacggggcgg gcctcgtgat
      841 gagcacgctc gacgtggtcg cacaggccgg cggcgagccc gccaacttct gcgacgtggg
      901 cggcggtgcc gacgccgagc agatcgctac ggccctcgac atcgtcacct ccaacgagca
      961 ggtggagagc gtgctcttca acatcttcgg cggcataacg cgcggcgacg aggtggcaag
     1021 gggccttctc tcggcgatag agcggaccgg gatcacactc cccatagtcg tcaggctcga
     1081 cgggacgaac gccgaggagg gacgccagat cctggccgag aacacgccgg agaacgtggt
     1141 cacggaggag acgatgctcg acgcggcccg gagggccgtg gagcttgcta gagacggggg
     1201 acggtagaga atggcgatac tcgtagacga agggacgcgg ctcctcgtac agggcataac
     1261 aggtagggag ggcgcgttcc atacagggag aatgctcgaa agcggcacga aggtggtcgc
     1321 cggggtgacg ccgggcaagg gcgggcagga ctacgagggc gtccccatct tcaacacggt
     1381 ggcagaggcg gtcgaagaga cgggtgcgaa cgcgagcgtc atcttcgtcc ccccgccgtt
     1441 cgccgccgat gccatcttcg aatccctgga cgctggcctc tcgaccatct gctgcatcac
     1501 ggaaggcatt cccgtgcacg acatgatgcg tgtcgccgac tacctcaaga tgaaagacgc
     1561 gaccctgatc gggcccaact gtcccggcgt cctctcgccg gggaagtcca acgtctccat
     1621 catgccccgc gacatcttca cgccaggcaa cgtcggcgtc gtcagccgta gcggtacgct
     1681 aacctaccag atcgtcaacg agctcaccca gagagacata gggcagagca ccgcggtcgg
     1741 tatcggcgga gaccccataa tcgggacgga cttcatcgag atactgagca agttcgaggc
     1801 tgaccccgag acagagtgcg ccgtgatgat cggggagata ggcggcaacg ccgaacagct
     1861 cgcggccgag tacgtccaga gcgagatgag cacccccgtc gtcgcctaca tcgctggctt
     1921 cacagccccc gagggcaaga ccatgggaca cgctggcgcg atcgtctcgg gcggcgagag
     1981 caccgccgaa gccaagagcg aggccctcag ggctgccggc atcagggccg cgacgaaccc
     2041 ttccgaggtc gccgagctcg tggccgaagc gctaggcaag ggctgacgcc cgaggaggcg
     2101 caaacggcga gagatcctgt ccccttgatc tccgataact tatacttcct gggctcacca
     2161 tgaacgggta ctgctcgacc caggcaagcc ggaccacgcc agcctccaag gcgggtgcat
     2221 agtggccgag gtctctaagg aacgcccggc caggcggttc atgcggtggc tcctcaggga
     2281 gcgcatcgag gaggtacagg gccccgaggc aagggaaggc gaagagcagc atgcctggtg
     2341 gcaggtcgtt tgcctgaccg gcgtcgacta cttctccact ctgggctaca taccaggaat
     2401 cgccgccctc gccgcaggct cgctctcgcc catcgccacg ctcctgatcg tcctgttgac
     2461 ccttttcgga atgctcccga tgtacagggt cgtcgccgcg gagagcccgc acgggcaggg
     2521 ctccatcgcg atgctggaac gactgctctc cttctggcag ggaaagttgt tcgtactctg
     2581 cctccttggc ttcgtcgcca catcctggat catcacgatc accctctccg cctccgacgc
     2641 cacggcccac gtagtagaga accctctggt ccccgaagcg ttcagaaacc acgaagtagc
     2701 catcacgatc atattgctcg ccgtgctcgg cgccgtcttt ctcaaagggt tcagggaggc
     2761 catcggtgtc gccgtcgcca tcgtcggggt ctatctcctc gtcaacatcg tcgtcgtcgt
     2821 tgccggcctg tatgagatct tcacccatcc cgcgaccttc atagggtggc aggacgcgtt
     2881 gttcaccaat tacggcaacc cgctgataat gatcggcgtc tccttgcttg tgttcccgca
     2941 actcgccctc gggctctctg gtttcgagac cggcgtgagc atgatgccgc tcgtgcgagg
     3001 cgaccctgat gacgaccacg agcagcccga gggacgtatc aggaacacgc gcaagatgct
     3061 cttcgtcgcg gccatcatca tgagcttcta tctgatcacg accagcttcg tgacctcggt
     3121 gctcatctcc gcacaagcgt tcgaggaagg tgccagcgat cgcgcgctcg cgtacctggc
     3181 gc
//
LOCUS       DATAIR010000139         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_49878, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000139 DATAIR010000000
VERSION     DATAIR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49878"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VHM69_03445"
     CDS             <1..342
                     /locus_tag="VHM69_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX2739478.1"
                     /translation="RPDLQQKVFDVIGIDPEEANRRFGTILTAFRYGAPPHGGIAPGV
                     DRLIMLLTDQPNIREVMAFPKTQAARDEMMDAPGPVSEEQLKELNISLRRRPEGERGS
                     DPASRPAEGNN"
     gene            407..1501
                     /locus_tag="VHM69_03450"
     CDS             407..1501
                     /locus_tag="VHM69_03450"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2739479.1"
                     /translation="MQTRFGASAPYTVGVEEELQLVDPASLELVPVIEEVLAARDAAG
                     LREDSVASELSASCVEVRTPAYATVAELATGLPALRGRVRDLAEGCGARLSAAGSHPF
                     SEATAQRITYEERYRKVDKEMGWPARMQAIYGLHVHVAVPDEERAILAVAALSRHVPL
                     FVALSANSPFWAGADTRLASVRTEVFGLVPRTGLPPAFRAWEEFEGYVDALVASGSIP
                     DYTLCWWDARPHPRLGTVELRAPDVQTDSTRTASLAALAQCLVATADEHPPENPLFTA
                     ENKWRATRHGLDASFHDFVSGRSSKARDSARTLVERLRPVAQDLGCENELDGVSEMAA
                     GGTGADRQRAAFMQRRSFKDVVEDLIAETA"
BASE COUNT          281 a          555 c          513 g          250 t
ORIGIN      
        1 cgccccgacc tgcagcagaa ggtcttcgac gtcatcggga tcgaccccga ggaggcgaac
       61 aggaggttcg gcacgatcct caccgccttc cgctacggcg ctccgccgca cggcgggata
      121 gcgccagggg tggaccgcct gatcatgctg ctcaccgacc agccgaatat acgcgaagta
      181 atggccttcc cgaagacgca ggcagcccgc gacgaaatga tggacgcacc cggtccggtc
      241 tcggaggagc aactgaagga gctcaacatc tccttgcgcc gccgcccaga gggcgaacga
      301 ggatcggacc cggcatcccg cccggctgaa ggcaacaact aggcttaaag ctgcggtcgg
      361 aggggactcc gggccacgga ccctgccatg ctgttaaggt agtctgatgc agaccagatt
      421 cggcgcttct gcgccgtaca cagtcggcgt tgaagaggag ctccagctcg tagatcctgc
      481 gtcgctcgag ctcgtccctg tgatagaaga ggtcctcgcc gccagagacg cggcggggct
      541 tcgcgaggac tcggtggcct ccgagctgtc ggcctcctgc gtggaagtac gcacgcccgc
      601 ctacgcgacg gtggccgagc tggcgaccgg gctgccggcc ctgcgcggac gtgtcaggga
      661 cctcgccgag ggttgcggag cccggctctc cgcggcgggg tcacacccct tctcagaggc
      721 taccgcgcag cggatcacct acgaagaacg ctaccgcaag gtggacaagg agatgggctg
      781 gccggcgcgg atgcaggcga tctacgggct gcacgtccac gtcgccgtcc ccgacgagga
      841 gcgcgccatc ctggccgtcg ccgcgctctc caggcacgtc cccctgttcg tggccctctc
      901 ggccaactcg ccattctggg ccggagccga cacccgcctt gcctcggtgc gtacggaggt
      961 cttcggcctg gtcccgcgca ccggcctgcc tcccgccttc cgcgcctggg aggaattcga
     1021 gggatatgtg gatgccctcg tcgcctcggg cagcatcccc gactacactc tgtgctggtg
     1081 ggacgcaagg ccccacccca gactcggcac cgtcgagctc cgcgcgcctg acgtgcagac
     1141 cgactccaca cgcaccgcct cgttggcagc gctcgcccaa tgcctcgtcg ccaccgccga
     1201 cgaacatccg cccgagaacc ccctgttcac ggctgagaac aagtggcgcg cgacccgcca
     1261 cgggttggac gccagcttcc acgatttcgt cagcgggcgc agttctaaag ccagggactc
     1321 cgccagaacc ctcgtagaga ggctgcgtcc cgtcgcgcaa gatctcggct gcgagaacga
     1381 gctcgacggt gtttcggaga tggccgcggg cggaaccggc gcagatagac aacgtgccgc
     1441 cttcatgcaa cgccgctcat tcaaagacgt cgtcgaagac cttatcgcgg agaccgcctg
     1501 aagtgtaatc aggcagccga gtagtttgcc ggcaggagct tgtgcgagat ccaccgggcc
     1561 atggccgccc tcgtggtgct tttttgtgga acgtacaac
//
LOCUS       DATAIR010000140         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_377326, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000140 DATAIR010000000
VERSION     DATAIR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377326"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..615)
                     /locus_tag="VHM69_03455"
     CDS             complement(1..615)
                     /locus_tag="VHM69_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2739480.1"
                     /translation="MNLLYPTLGALVVLAVLTFLIGRQNRDLLRVEAYRDFFLRLIPP
                     LSTGVFIAGLPFVMDLATVENYGPVLLVYLGGAAVLTALMTRAVTPDERRAGTAFRKG
                     EYEKAAATYDEIVSRRPLPRYYSALGASLDASGNPGAALEATDQAIKLDPRLGIAYYN
                     RASALASLGEKASARADLQKVFQVDSSRSLRRAAAEALASLEKR"
     gene            complement(612..1214)
                     /locus_tag="VHM69_03460"
     CDS             complement(612..1214)
                     /locus_tag="VHM69_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2739481.1"
                     /translation="MGSGAITIVLIFLLLFVSWQTIFVSNKDLARVKAWGDLFRRSLP
                     FFALLVMVLTLFVIQTYQQGGLLVGWMVVSGVAIVLASLLAIPSLERRASKAFRGGDY
                     ARAAELYGELAEEKPLARNNAFLGAALGASERYEESVDASTKAIELDPKYGLAYYNRA
                     LVLQRMGKKSGAVKDLKKALEADLPRRFRSAAQKMLDELT"
     gene            complement(1223..1807)
                     /locus_tag="VHM69_03465"
     CDS             complement(1223..1807)
                     /locus_tag="VHM69_03465"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX2739482.1"
                     /translation="MDSLQHALKEWAVAVMALERGETALVVRKGGIREKAFAVPRTRF
                     LLLPGYEHQRPDLLKPEYRELMREIPSLTDDGPLRFSSFAEVEGAYEVSEAEDLTSLD
                     PYHMWTPEYAGSRFKWRPKKPLTVLVLRTYLMPESVELPYVDEYGGCKSWIELQETVS
                     VEGARPALSDEDFDELVAPALGVLRGLEPAPVGT"
     gene            1881..3278
                     /locus_tag="VHM69_03470"
     CDS             1881..3278
                     /locus_tag="VHM69_03470"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX2739483.1"
                     /translation="MTQQETPHRVERDSMGEIEVPRDALFGAQTRRALDNFPISDLRF
                     SRRFIQALGAIKLEATNVNNELGLLDDELKDAIVEAAEEVVEGRLDNQFVLDIFQTGS
                     GTSTNMNANEVISNRAIQLVGGELGSKDPLPPNDHVNQGQSSNDVIPTAIHLAALIAI
                     KEDLLPGLQKLQSALEEKADEFDDVVKTGRTHLQDATPIRLGQEFRGYAGQIERGVQR
                     VESALEDLAEVALGGTAVGTGVNTHPEFASKVAERLSERFGVEVRETENHFQAQSAMD
                     AAVFTSGALKTVAVSLLKISNDIRWLGAGPRANLAEMALPEVQPGSSIMPGKVNPVIA
                     ESATMVAAQVIGNDATIVVAGQSGNFELNVMLPVIAYNLIQSIEILGSAASNLTDQCV
                     AGLEATERGPALVEQGLMLATALAPVVGYDKAAEISKEAYKTGKTIREVAREKTDLSE
                     DKLDELLDARKMTQS"
BASE COUNT          686 a         1099 c         1066 g          563 t
ORIGIN      
        1 ctatctcttc tccagcgacg ccagggcttc ggcggcggcg cgacggaggg aacggctgga
       61 gtcgacctgg aagaccttct gcaggtcggc gcgtgcgctg gccttctcgc ccaacgaggc
      121 gagggctgag gctctgttgt agtaggcgat accgagtctg gggtcgagct ttatcgcctg
      181 gtccgtagct tcgagggcgg cgccagggtt gcccgaggcg tcgaggctcg cccccagtgc
      241 cgagtagtac cgtgggaggg gacggcggct gacgatctca tcgtaggtgg cggcggcctt
      301 ctcgtactcg cccttgcgga aggcggtacc ggcccggcgt tcatcggggg tgacggcccg
      361 ggtcatcagg gccgtaagta cggcggcgcc gccgaggtag accagcagga cggggccgta
      421 attctcgacg gtcgcgaggt ccatcacgaa ggggaggccg gctataaaca cgcccgtgga
      481 gaggggtggg atcagccgca ggaaaaagtc cctgtaggcc tccacgcgca acaggtcccg
      541 gttctggcgg ccgatcagga aagtgaggac cgcaaggact actagcgcgc cgagcgtagg
      601 atagagaagg ttcacgtgag ctcgtccagc atcttctgcg cggccgacct gaagcgtcgg
      661 gggaggtcag cctcaagcgc ctttttgagg tccttgacgg ccccgctctt cttccccatc
      721 ctttgcaaga cgagggcgcg gttgtagtaa gcgaggccgt acttcgggtc cagctctatc
      781 gccttcgtcg aggcgtccac ggactcctcg tagcgctccg aggccccgag agcagcccct
      841 aggaaagcat tgttgcgggc gagtggcttc tcctccgcca gctccccgta cagttccgcc
      901 gccctcgcgt agtcgccgcc gcggaaggcc ttgctggccc tgcgttccag ggaggggatg
      961 gcgaggaggc tcgcgaggac tatcgcgacg ccagagacga ccatccatcc cacgagcaac
     1021 cctccctgct ggtaggtctg aatcacgaag agggtcaaga ccatcaccag cagcgcgaag
     1081 aaggggaggg acctgcggaa gaggtctccc caggccttca ccctggccag gtccttgttg
     1141 gagacgaata tcgtctgcca gctcacgaag aggagaagga agatcaagac tatagttatg
     1201 gctcccgaac ccatatcccc tcttaagtgc cgaccggcgc gggctctagg ccccgcaaca
     1261 ccccgagcgc aggggcgacc agctcgtcga agtcctcgtc ggagagcgcg ggccgcgccc
     1321 cctcgaccga gacggtctcc tgaagttcga tccaggactt gcatcccccg tactcatcca
     1381 cgtacggaag ctcgacggac tccggcatca aataggtgcg caagacgagg accgtgagcg
     1441 gcttcttggg gcgccacttg aagcggctcc ccgcgtactc gggcgtccac atgtggtacg
     1501 ggtcgagcga cgtgagatcc tccgcctcgg agacctcgta agctccctcg acctcggcga
     1561 acgacgagaa gcgcaagggg ccgtcgtccg tgagggaggg gatctcccgc ataagttccc
     1621 gatactccgg cttcagaagg tccggcctct ggtgctcgta gccggggagg agcaggaatc
     1681 gcgtccgagg caccgcgaaa gccttctccc taatgccgcc tttcctgacg acgagcgccg
     1741 tctccccgcg ctccagtgcc atcaccgcga ccgcccactc cttcagcgcg tgctgtaaag
     1801 aatccaagac atcacctctc gtataatgcg accgttgcac gccagattat atgactaatc
     1861 gcttcgacag gaggcttcac atgacgcagc aagagacgcc acaccgcgta gagcgagact
     1921 cgatggggga gatcgaggtc ccccgcgacg ccctcttcgg cgcccagacc cgccgcgccc
     1981 tggataactt ccctataagc gacctccgct tctctcgccg cttcatccag gccctcggcg
     2041 ccataaagct ggaggcgacc aacgtgaaca acgagcttgg tttgctcgac gacgagctca
     2101 aggacgccat cgtcgaggcg gctgaggagg tcgtcgaggg acggctggac aaccagttcg
     2161 tgctcgacat cttccagacg gggagcggca cgtctaccaa catgaacgcc aacgaggtca
     2221 tttccaacag ggccatccaa ctcgttggcg gagagctcgg ctcaaaagac ccgctccctc
     2281 ccaacgacca cgtcaaccag ggccagtcct cgaacgatgt gatcccaacg gccatccacc
     2341 tcgccgccct catcgctatc aaggaggatc tgctccccgg cttgcagaag cttcaaagcg
     2401 ccctcgaaga gaaggcagat gagttcgatg acgtagtcaa gacgggacgg acgcatcttc
     2461 aggacgccac cccgatccgt ctcgggcagg agttccgggg ctacgccggc cagatcgaac
     2521 gcggagtgca gcgcgtcgag agcgccttag aagacctcgc cgaagtcgcc ctcggaggca
     2581 cggccgtcgg cacaggcgtc aacacccacc ccgagttcgc ctcgaaagtt gccgagcgtc
     2641 tatccgagcg cttcggcgtc gaggtgcgtg agacggagaa ccacttccag gcccagagcg
     2701 cgatggacgc agccgtcttc acgagcggcg ccttgaagac tgtcgcggtg agcctgctca
     2761 agatctccaa cgacatccgc tggctcgggg ccgggcctcg cgccaacctc gccgagatgg
     2821 cgctccccga agtgcagccc ggttcatcca tcatgcccgg caaggtgaac cctgtgatcg
     2881 ccgagagcgc aacgatggtc gcggcgcagg tcataggcaa cgacgccacc atcgtagtgg
     2941 cggggcagag tggcaacttc gagctcaacg tcatgctacc cgtgatcgcg tacaacctga
     3001 tccaaagcat agaaatattg ggttccgctg cttccaacct caccgaccag tgcgtcgctg
     3061 gcctcgaagc gacggagcgc ggccccgccc tcgtcgagca gggcctcatg ctcgccacgg
     3121 ccctcgcccc cgtggtcggc tacgataaag cggccgagat ctccaaagag gcgtacaaga
     3181 caggcaagac aatccgcgaa gtcgcacgcg aaaagaccga cctctccgaa gataagctgg
     3241 acgagctact ggatgctcgc aagatgacgc aatcgtagcc ggaatggtga atagaaagta
     3301 gctctcatgg tgtcataata tgatactatg aaactcggta gcaagcatcg caagacgctc
     3361 cgggccgtct tcgaggatcc ggtccgttcg gatgtagcgt gggccgacgt ggag
//
LOCUS       DATAIR010000141         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_100674, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000141 DATAIR010000000
VERSION     DATAIR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100674"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VHM69_03475"
     CDS             <1..1222
                     /locus_tag="VHM69_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HEX2739484.1"
                     /translation="GILGAVVGDQIGYWIGREGGRPFVLRWGRYVFITPERLARAEAF
                     FARHGGKAVFMARFFSGLRVFGALIAGMSRMRWGTFIFYNALGGAVWATAAVLVGYFL
                     GSSLGVVERWTGRASILLLALALLALVLYLTYRWVTRHPEQVKRAFERIGGRRVYALL
                     ESPVGLWLKRRFSPNEVYGLTLTVGFILMGLFSWAFGGIVQDVIASDPLVRLDLTVLR
                     FFHSHGEPYLTTSVEVLEAVFSPVVLLLVATLAGFGFLLLARGDFDAGLSGAALLATA
                     FGTVVLSQLLKALFHRPRPPASLQLVHETGYGFPSTHAMAVVALGAVVWYIFGLRPLE
                     RWGGSWRARTRIGVAVVAVSLLVGLGRVYTGAHYPSDVLAGWALGGVWASVCLTAAEV
                     LRRLREEKIGDSGG"
     gene            1262..>1783
                     /locus_tag="VHM69_03480"
     CDS             1262..>1783
                     /locus_tag="VHM69_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2739485.1"
                     /translation="MLEAILHPLIEFVTETIGSYGVPAVFVLMLLESMGILIPSEAIS
                     PFAGYLVSEGKMSLFAAVAAGVLGNLVGSWIAYFIGLWGGRELWFHYGKYVGVRAHHL
                     HIAEKWFDKYGEFTVFISRCLPVVRTFISFPAGTAKMNFAKFSFYTLLGCIPWVFALT
                     YLGYVLGENWETIG"
BASE COUNT          257 a          558 c          574 g          394 t
ORIGIN      
        1 cggcatcctc ggcgctgtgg tgggggacca gatcggctac tggataggcc gcgaaggggg
       61 acgccccttc gtcctcaggt ggggacgata cgtctttatc acccccgaac gcctcgcacg
      121 cgccgaagcg ttcttcgcca ggcacggggg gaaggccgtc ttcatggcgc gcttcttctc
      181 gggcctcagg gtatttggag cactgatcgc agggatgagc cgtatgcgct gggggacttt
      241 catcttttac aatgccctcg ggggagcggt gtgggccacg gcggccgtcc tggttggata
      301 cttcctcggt agcagcctcg gtgtcgtgga gcgctggacg gggcgggcct ccatactgct
      361 actcgcgctc gccctactgg ctctggtcct ctacctgact taccggtggg taacacgtca
      421 tcccgagcag gtcaaaaggg cgttcgagcg gatcgggggc cggcgcgttt acgctctact
      481 ggagagcccg gtaggactct ggctcaagcg gcggttctcc cccaacgagg tctacggtct
      541 cacgctcacg gtcggattca tcctcatggg acttttctcc tgggccttcg gcggcatcgt
      601 gcaggacgtg attgccagcg accctctcgt gaggttggat ctcacggtgc ttcgtttctt
      661 ccactcgcac ggcgagcctt acctcaccac cagtgtggag gtgctggagg ccgtcttctc
      721 accagtggtg ctcctactgg tggcgaccct cgctggcttc gggtttcttc tcctcgcacg
      781 cggggacttc gacgcgggac tctccggggc cgcgctcctg gcgacggcgt tcggcacggt
      841 cgtcctttcg cagttgctca aagccctctt ccacaggccc agaccgcccg cttccctgca
      901 gctggtgcac gagaccggct acggttttcc cagcacccat gcaatggctg tggtcgccct
      961 cggggcggtc gtctggtaca tcttcggcct gcgtcctctc gaacgctggg gcgggtcatg
     1021 gcgggccagg acgaggatag gggtggccgt cgtggcggtc tcgttgctgg tcggtctggg
     1081 tcgcgtgtac acaggggcgc actatccgag cgacgtcctg gcaggatggg cgctcggcgg
     1141 cgtctgggcc tccgtctgcc tgacggctgc cgaggtccta agaaggctgc gggaggagaa
     1201 aatcggcgat agcggcggat agtcgttgta cattctcgcc ctcaagtggt agtttctccc
     1261 cgtgcttgaa gcaatcctac atccgctcat agagttcgtc accgagacca taggcagcta
     1321 cggcgtcccc gcggtcttcg ttctcatgct gctcgagagc atgggcatcc tgatcccttc
     1381 cgaggcgatc tcacccttcg ccggctacct ggtctcggag gggaagatga gcctgtttgc
     1441 tgccgtcgcc gccggtgttc tcggaaacct cgtcgggtcc tggatcgcct actttatcgg
     1501 gctctgggga gggcgcgagc tctggttcca ctacggaaaa tacgttggcg tgagggcgca
     1561 ccaccttcac atcgccgaga agtggttcga caagtacggc gaattcaccg tgttcatctc
     1621 ccgctgcctg cccgtggtcc gcacgtttat ctcgtttccc gccggcaccg ccaagatgaa
     1681 cttcgccaag ttcagcttct acaccctgct cgggtgcatc ccgtgggtct tcgcgctgac
     1741 ctacttgggg tacgtgctcg gtgagaactg ggagaccata ggg
//
LOCUS       DATAIR010000142         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_423618, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000142 DATAIR010000000
VERSION     DATAIR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423618"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..435
                     /locus_tag="VHM69_03485"
     CDS             70..435
                     /locus_tag="VHM69_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2739486.1"
                     /translation="MPRLEVEGVAPVKVEDGKRLVLAIEEDAGVDIQHQCGSYAHCTT
                     CRVEFLEGEPEDMTQAELLALEMRDLVEKMRLSCQIYCEEDMKLKVGLTVSGSKANSA
                     GGKPEEEITPEPVWIERPY"
     gene            511..840
                     /locus_tag="VHM69_03490"
     CDS             511..840
                     /locus_tag="VHM69_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739487.1"
                     /translation="MENSGGSVQAAKEPVISEWVGYWVFVSYMAGPNLDADEPAKPVR
                     DKPEAVTASFLLEDYGDLGIEVKRNPTDPTIFMSWGAVLYVQGPPPEVRKQIDLENAK
                     QAKGNGK"
     gene            876..2021
                     /locus_tag="VHM69_03495"
     CDS             876..2021
                     /locus_tag="VHM69_03495"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEX2739488.1"
                     /translation="MQENGGRPLTAVFASPGKYIQGAGAVGRIGEVLEQLGSEKPLIL
                     GDPIVEEIMGGTLDNISGATRTEFGGECSPQEIDRVAAAASENGADALVGIGGGKTID
                     TAKAVAHPAGLPLVIVPTIASTDAPTSAVSVVYEEDGAFKEYRFFSKNPSAVVVDTAV
                     IASAPVRFLVAGIGDGLSTYFEADASSRTRKQTMAGGAPTLASLALARLCYDTLLEHG
                     LAARLAVEKQAVTPAVEKVVEANTLLSGLGFESGGLAAAHSIHNGLTALEGTHHYWHG
                     EKVAFGVISMIMLEERPAEVLAEVVDFCLEVGLPVTLEDIGLDDVSREDLEKVAEAAC
                     IEGETIHNMPIEVHPPMVVDAMIAADAYGHQRRALLAGEAALPEVAR"
BASE COUNT          422 a          657 c          743 g          340 t
ORIGIN      
        1 aaggaggcgt cggcgctgat ccagaacggg cggtagggag atgggcccac aaagcgagag
       61 ggggacggtt tgcccaggtt agaggtcgaa ggcgtcgcgc ccgtcaaggt cgaggacggg
      121 aagcggctgg tcctcgccat agaggaggac gcaggcgtag acatccagca ccagtgcggc
      181 agctacgccc actgcacgac gtgccgggtc gagttcctgg aaggggaacc cgaggacatg
      241 acgcaggccg agctactggc gctggagatg cgggacctcg tggagaagat gaggctgtcg
      301 tgccagatct actgcgaaga ggacatgaaa ctgaaagtcg ggctcaccgt gagcgggagc
      361 aaggcaaact cggcgggagg caagcctgaa gaggagatca cgccggagcc cgtctggata
      421 gagaggccgt attgaaggcg acagctatca gcggtcggct ttcggctcct ggctggaaag
      481 cggagatgtg tccgtcaggg ggaggggtac gtggagaata gcggcggaag cgtgcaggcc
      541 gcgaaggagc ccgtgatctc ggagtgggtt gggtactggg tcttcgtctc gtacatggcg
      601 gggccgaacc tggacgccga cgagccggcc aagcctgtca gggataagcc tgaggccgtg
      661 accgcctcgt tcttgctcga agattatggg gacctgggca tcgaggtgaa gcgcaatccc
      721 acggacccga cgatctttat gtcctggggg gccgtactct acgtgcaggg gccgccgccg
      781 gaggtgagaa agcagatcga cctcgaaaac gcgaagcagg caaagggcaa cggaaagtaa
      841 ctggtacgtc cactaactgt aaggagaacg agctgatgca agagaacggt ggaaggcctc
      901 tgacggccgt gttcgcgtcg cccggcaagt acatccaggg tgccggtgct gtcgggagga
      961 tcggggaggt tctcgaacaa ctcgggtcgg agaagcccct gatcctgggc gacccgattg
     1021 tcgaggagat catgggcgga acgctggaca atatctccgg cgcgacccgc accgagttcg
     1081 gcggcgagtg ctcgccccag gagatagacc gggtggcggc cgcggcctcg gagaacggcg
     1141 ccgatgccct tgtcggcatc ggtggcggca agaccataga caccgccaag gccgtagccc
     1201 acccggcggg gctgccgctc gtcatagtac cgaccatagc ctcgaccgac gccccgacga
     1261 gcgccgtctc cgtcgtctac gaggaggacg gcgctttcaa ggagtaccgc ttcttcagca
     1321 agaacccgtc ggccgtcgtc gtcgacacgg cggtcatagc cagcgccccg gtccgtttcc
     1381 tggtcgccgg tatcggggac ggcctgagca cgtacttcga ggccgacgcc tcgtctcgta
     1441 cccgcaagca gacgatggcg ggcggcgccc cgacgctcgc ctccctcgcc ttggcccgcc
     1501 tctgctacga caccctcctc gaacacggcc tcgccgcccg cctcgccgtc gagaagcagg
     1561 cggttacgcc cgccgtcgag aaggtcgtcg aggcgaacac gcttctctcc ggactcggct
     1621 tcgagtcggg cgggctcgcg gcggcccact ccatccacaa cggcctcacg gcgctcgaag
     1681 gcacgcacca ctactggcac ggggagaaag ttgcgttcgg ggtcatctcc atgatcatgc
     1741 tggaggaacg ccccgccgag gtccttgccg aggtcgttga cttctgcctc gaagtgggtc
     1801 tccccgtaac cctcgaagac ataggcctcg acgacgtttc gcgcgaggac ctcgaaaagg
     1861 tcgcggaggc ggcgtgcatc gagggggaga cgatccacaa catgcccatc gaggtccacc
     1921 cgccgatggt ggtcgacgcc atgatcgccg ccgacgccta cggccatcaa cgccgcgccc
     1981 tgctcgcggg cgaagccgcg ctgccagagg tcgcgcgctg atagcgagtc cggtcggaga
     2041 gtatgtcact cgagtgcgta cgtagggagt agagaaaatt actactccct actcctttag
     2101 acttgcggtc tcgaggtctt caaactgctc ccgcctcagg cagagccgtt cagcctctgt
     2161 tt
//
LOCUS       DATAIR010000143         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_49976, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000143 DATAIR010000000
VERSION     DATAIR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49976"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..536)
                     /locus_tag="VHM69_03500"
     CDS             complement(333..536)
                     /locus_tag="VHM69_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739489.1"
                     /translation="MVGRLMNGGAGEVLVVSLSGMIAVMVANGPGRASLGRVLSGALS
                     NPVPRRETSGGVSPTGAERRAAA"
     gene            complement(607..2013)
                     /locus_tag="VHM69_03505"
     CDS             complement(607..2013)
                     /locus_tag="VHM69_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2739490.1"
                     /translation="METIIQGSTLETLRAGLRGTAYAPGEEGYEEASRAWNLNAQQRP
                     ALVVVAEGASDVMTAVRFAREGGLGVGVMATGHGTGTECDGGVLINTSRMRGVRVDPV
                     ARTARVEAGALWKDVIPEGQAHGLAGLVGSSSNVGVVGYTMGGGFGWLGRKYGLNSAG
                     VREADVVTAEGKFLRVSAEENEDLFWGLKGGGGNFGIVTSLVFDLYPLTSVYGGNVFY
                     PVEKAGEVLARYASWSADLTDETTTGIAFLNIPPLPFLPEPLRGKSVIAVRGCYSGES
                     PEAGEELFRAVREGLGEPIMDTFGVMPYAAMDAISMDPVDPIGAVQYSELLQDLTPEA
                     IDALVEVAGAGSGSPLIVLELRQLGGALSGTPEDLNPMGSGDARFIMNGVGATFTPEM
                     MEGVRAHLARVAEATRPFQTGDTYVNFMELDFAGADRVKAAYPPEDFERLVALKDRYD
                     PNNVFRYNRNIAPSKSEG"
     gene            complement(2158..3126)
                     /gene="corA"
                     /locus_tag="VHM69_03510"
     CDS             complement(2158..3126)
                     /gene="corA"
                     /locus_tag="VHM69_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX2739491.1"
                     /translation="MIVDNAIYVDGHRTEEPGTLGETYEAVRERRGVAWIGLYRPTEE
                     EFSSVAGEFGLHGLAVEDATEAHQRPKLERYGETLFVVLKPARYLDEEERVEFGEVHV
                     FVGDDFVVTIRHGEAPDLGEVRKQLEADPELLRRGPETILHAIMDRVVDDYAPVVDGL
                     GNDIQEIEAEVFGGSPNVSRRIYELSREVIEFQRATEPLDGMLGDLIREETIQVDPEV
                     RRYLRDVKDHVLQVTERLAGFRELLQNILSVNLTLASLSQNEEVKKISAWAAILFAPT
                     LIGTVYGMNFRYMPELNWFFGYPFALLLMGLVSLTLYLVFKRRGWL"
     gene            complement(3160..3969)
                     /locus_tag="VHM69_03515"
     CDS             complement(3160..3969)
                     /locus_tag="VHM69_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739492.1"
                     /translation="MSAATGNVRSRRYGGLYWAFADGFVLAKRNLVQIPRIPELVVFA
                     TIQPVMFVLLFRYVFGGAIDVGGESYVNYLMAGIFVQTVAFGSVTTGIGLSEDLHKGL
                     VDRFRSLPMAHSAVLTGRTIADLVRNLFVVLVMLLVGILVGFRPEAGLAGWAAAIGLL
                     LLLSFAFSWIGATIALLMRSTEAVQSAGFIWLFPLTFASSAYVQTNNMPGPLRAFANH
                     QPMTQVMDAVRGLLLGQSVGSHGWLALAWCVGILVIFIPLSVSLYRRVATR"
     gene            complement(3966..4967)
                     /locus_tag="VHM69_03520"
     CDS             complement(3966..4967)
                     /locus_tag="VHM69_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2739493.1"
                     /translation="MTEGRTAILAEGLRKSYGKTLALDGLDLEVREGTVLGMLGPNGA
                     GKTTAVRIFTTLLKPDSGRAEVAGLDVVRQADELRSRIGLTGQYAAVDEYLTGRENLE
                     MVGRLYHLPKKEARRRADELLERFDLVDAASRLVKTYSGGMRRRLDLSASLVLSPPVL
                     FLDEPTTGLDPRSRLAMWDIIGELVSGGTTLLLTTQYLDEADRLADRIAVVDAGRVIA
                     DGTSSELKAQVGGERLEVTVARGDGLEDAARVLESYAHGDGGVRVDVERRYVVATVAG
                     GAGLLAAVVRDLDAAGVLVEELGLHRPTLDDVFLILTGHAAEPEATDDERPDEKEIA"
     gene            complement(5067..>5585)
                     /locus_tag="VHM69_03525"
     CDS             complement(5067..>5585)
                     /locus_tag="VHM69_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2739494.1"
                     /translation="AKARQLGARIVEEPFDVMGLGRMAVFADPGGAMLCVWEPQAHIG
                     AGRVNDVGCMGWNELQTRDSGAAGDFYSVLFGWEIEPIEQDGMLVYTTIKNSGSQNGG
                     FMPMSEQHGDAPSFWLPYFTVSSRDGAVKKVQELGGALLAGPLDLPSGKVAIVSDPQG
                     AAFAIFEGETDE"
BASE COUNT          990 a         1880 c         1726 g          989 t
ORIGIN      
        1 ggtaagtaaa gagtcgacat agagcctaca cgcattccaa agcctcatgt actccccgaa
       61 tatcgaggct gattgtaaat ctcaaatcat ctagcacact gtagaggtcc atactatccg
      121 cacgcagtgc agcaagtggt aggcctgagg cgggagttgg tagagtacac tcctcagggc
      181 tccaagtcgc ttgcttagcg gctagccttg gtcgggccgg ttcaacatgg cccgggtagc
      241 cgcggtgaca tgatgccagg tcagtctgaa gaccggtgtg actctcgctc tgtgctcgac
      301 ccggcttgcg ccgtgccgcg ggcggcctgg gatcaggcag ccgcccgccg ttcggcgccg
      361 gtcggcgaga ctccgccgga ggtctcgcgc cggggtaccg gattggacag ggcccccgac
      421 agtacacgcc cgagcgaggc gcggcctggg ccgttggcca ccatgaccgc gatcatcccg
      481 gagagggaga ccacgaggac ttcgcctgcc ccgccattca tcagtctgcc gaccatcctt
      541 tctcctttcg cgttcgatat atcccgaccg ggggccgcat gcgacccccg atcaatgttt
      601 cagcgcttat ccttccgact tcgatggggc gatgttgcgg ttgtagcgga acacgttatt
      661 cgggtcgtag cggtccttca gggcgacgag gcgctcgaag tcctcgggcg ggtaggctgc
      721 cttgaccctg tctgcgcccg cgaagtcgag ctccataaag ttgacgtagg tgtcgccggt
      781 ctggaaagga cgcgtcgcct ccgccacgcg ggcgaggtgc gctctgaccc cttccatcat
      841 ctcgggtgta aaggtcgccc cgaccccgtt catgatgaac ctggcgtcgc ccgagcccat
      901 cgggttcaga tcctctggcg tacccgagag tgccccgccg agctgacgga gttcgagcac
      961 gatcaggggc gaccccgatc cggcacccgc gacctctacg agagcgtcta tggcctcggg
     1021 cgtgaggtcc tgcagcaact cagagtactg gactgcgcct atcgggtcca cagggtccat
     1081 gctgatcgcg tccatcgccg cgtagggcat gaccccgaat gtgtccatga tcggctcccc
     1141 gagcccctcg cgcacggcac ggaagagctc ctctccggcc tcgggcgact ctcctgagta
     1201 gcatccccgc accgctatga ccgacttgcc acgcaggggc tcggggagga acggaagagg
     1261 cgggatgttg aggaaggcga tccccgtggt cgtctcgtcc gtcagatcgg cgctccagct
     1321 cgcgtaacgg gcaagcacct cgcctgcctt ctcgaccgga tagaatacgt tgcctccgta
     1381 gacactcgtc agagggtaga ggtcgaacac gagggacgtg actatcccga agttaccgcc
     1441 accgcccttg agaccccaga acaggtcctc gttttcctcg gcgctcacgc gcaagaactt
     1501 gccttcggcg gtcacgacgt cggcctcacg cacgcccgcg gagttcaagc cgtacttgcg
     1561 cccgagccac ccgaacccgc cgcccatcgt gtagccgacg accccgacgt tggaggatga
     1621 gcccacgagc ccggcgagcc cgtgcgcctg tccctcgggg ataacgtcct tccacagcgc
     1681 acctgcctcg acccgcgcgg tccgcgccac ggggtctacc ctgacgcccc tcatgcggga
     1741 ggtgttgatg aggaccccgc cgtcgcactc cgtccccgtg ccgtggcccg tcgccatcac
     1801 cccgacgcca agtcctcctt cacgggcgaa acgcacggcg gtcatcacgt cggaggcgcc
     1861 ttcggccacg accaccagcg cgggacgctg ctgagcgttg aggttccacg cccggctcgc
     1921 ctcctcgtag ccctcctcac ccggcgcgta tgcggtgccg cgcaggccgg cccgcagcgt
     1981 ctctagcgtc gagccctgga ttatcgtctc cataatcgtc tcccgtctct ctcacggttg
     2041 cgagccggtt ggccattcct accggccggt tcaggcaacg gatgcagcct agctttcgag
     2101 gttagacgac acatccccca aatgggctat ctttcgggat gcgggtggag cgaaaagcta
     2161 aagccagccg cggcgtttaa agaccaggta gagcgtgagg gagacaagcc ccatgagcaa
     2221 cagggcgaag gggtagccga agaaccagtt cagctcgggc atgtagcgga agttcatacc
     2281 gtagacggtt ccaatgagcg tgggcgcaaa gaggatggct gcccaggcgg agatcttctt
     2341 tacctcctcg ttctgggaga gactggccag ggtcaggttc acgctgagga tgttctgcag
     2401 cagctcgcgg aacccggcga gccgttccgt gacctgcaag acgtggtcct tgacgtcgcg
     2461 caggtacctc cttacctcag ggtcgacctg tatcgtttct tcccgaatca ggtcccccag
     2521 catgccgtcg agcggctccg ttgccctctg gaattcgatg acctcgcgcg agagctcgta
     2581 gatcctgcgc gacacattgg ggcttccccc gaaaacctcg gcctctatct cctggatgtc
     2641 gttgcctagg ccgtcgacca caggggcgta gtcgtccacg acgcggtcca tgatggcgtg
     2701 cagaatagtc tcaggtcctc tgcgcaggag ctcgggatca gcctccaact gcttgcgtac
     2761 ctcgccgagg tcgggggcct cgccgtggcg gatggttacg acgaaatcgt cccccacgaa
     2821 gacgtggacc tccccgaact cgaccctctc ctcctcgtcc aggtaacggg caggtttgag
     2881 aacgacgaag agggtctcac cgtagcgctc cagcttgggt cgctggtggg cctcggtcgc
     2941 gtcctcgacg gccagcccgt gcaggccgaa ctcccccgcg acggaggaga actcctcttc
     3001 cgtcggcctg tagaggccga tccaggccac cccccgccgc tccctgacgg cctcgtacgt
     3061 ctccccaagg gtgcctggtt cctcggtcct gtggccgtcc acgtagatgg cgttgtccac
     3121 gatcacgggc gcattatacg cccgtgatcc tcgtccgcct cagcgcgtgg cgacgcgcct
     3181 gtacagggag acggagaggg gtatgaagat aacgagtatc ccaacgcacc aggcgagggc
     3241 cagccatccg tgcgagccga cggactggcc gaggagcaac ccacgtaccg cgtccatgac
     3301 ctgtgtcatc ggctggtggt tggcgaaggc cctcaagggg cctggcatgt tgttcgtctg
     3361 gacgtacgcg ctgctcgcga aggtcaaagg gaatagccag atgaagcctg ccgattgcac
     3421 ggcctcggtg ctgcgcatga ggagtgcgat cgtcgccccg atccatgaaa atgcgaagct
     3481 gagcagcaac agaagcccga tggccgcggc ccagccagcg agaccggcct cgggtctgaa
     3541 ccctacgagg atgccgacga ggagcatcac gagcacgacg aaaaggttgc ggacgaggtc
     3601 ggcgatggtg cggccagtga ggacggcgga gtgggccatg ggcaacgagc ggaaccggtc
     3661 gacgagtccc ttatggaggt cctcggacag cccgatgccc gtggtcaccg acccgaaggc
     3721 gacggtctgg acgaagatcc ccgccatcaa atagttgacg tagctctcgc cgccgacatc
     3781 gatcgcaccc ccgaacacgt aacggaacag gagcacgaac atgacaggct gtatcgtcgc
     3841 gaagaccacg agctcgggta tgcgtgggat ctggacgagg ttgcgctttg ccaggacgaa
     3901 tccgtcggcg aaggcccagt agagcccgcc gtaccgacgt gaccgcacgt tgccggtcgc
     3961 cgcgctcatg cgatctcctt ctcgtcgggc ctctcgtcat ctgtggcttc gggctccgcc
     4021 gcgtggcccg tgaggatgag gaagacgtcg tccaaggtgg gacggtgcag gcccagttct
     4081 tcgaccagca cccctgcggc gtcgaggtcg cgtacgacgg cggccagcaa ccccgcacct
     4141 cccgctaccg tcgctaccac gtacctgcgt tcgacatcga cgcgcactcc gccgtcgccg
     4201 tgggcgtaac tttcgagtac cctggcggca tcctcgagcc cgtcaccccg tgccacggtc
     4261 acctcgaggc gctcgccgcc tacctgcgcc ttgagctcgc tagatgtacc atcggcgatc
     4321 acacgccctg cgtcgactac ggcgatcctg tcggcaagac ggtccgcctc gtcgaggtat
     4381 tgcgtcgtga ggagcagcgt cgtcccaccg gagacgagct ccccgatgat atcccacatg
     4441 gcgagacggc tcctcgggtc caaccccgtg gtcggctcgt cgaggaagag cacgggcggg
     4501 gagaggacga ggctcgccga gaggtcgagg cggcggcgca ttccgcccga gtacgtcttt
     4561 acgagccgag aggcggcgtc cacgaggtcg aagcgctcga ggagctcgtc ggccctcctg
     4621 cgcgcctctt tcttcggcag atggtagagc cgcccgacca tctcaaggtt ctcgcgaccc
     4681 gtgaggtact cgtctacggc ggcgtactgg cccgtgagcc cgatgcgcga gcgcagctcg
     4741 tcggcctgcc gcacgacatc gaggcccgcg acttcagccc gcccggagtc aggcttcagt
     4801 agcgtggtga agatgcggac cgctgtcgtc ttgccagcgc cgttcggccc gagcatcccg
     4861 aggaccgtac cctcccgaac ctccaggtcc agaccgtcca gcgccagggt cttgccgtag
     4921 ctcttcctca gaccttcggc cagtatggcg gttctgcctt cagtcacgcg atgcccttcc
     4981 acgagtattc ttcgctgatc aaactagaaa agtcggctga tacacctgaa gatctcacaa
     5041 tcctcgcagg tttatgactg cataccctat tcgtcggtct cgccttcgaa gatcgcgaac
     5101 gccgcgccct ggggatcgct cacgatggcg accttgcctg aaggtagatc cagcggcccg
     5161 gccagcaaag caccgccaag ctcctgcacc ttcttgacgg cgccgtcgcg cgaggagacc
     5221 gtgaagtaag gtagccagaa ggacggcgca tcgccgtgct gctccgacat cggcatgaag
     5281 ccgccgttct gagaccctga gttcttgatc gtggtgtaga ccagcattcc gtcctgctcg
     5341 attggctcga tctcccagcc aaaaagcacg ctgtaaaagt cgcccgccgc ccccgagtca
     5401 cgggtctgga gctcgttcca gcccatacag ccaacgtcgt tgacccgtcc ggcgccgatg
     5461 tgcgcctgtg gctcccacac gcagagcatc gcgccaccgg gatcggcgaa aacggccatg
     5521 cggcccaagc ccatcacgtc gaagggctcc tcgactatcc tggcgccgag ttgcctcgcc
     5581 tttgc
//
LOCUS       DATAIR010000144         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_8604, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000144 DATAIR010000000
VERSION     DATAIR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8604"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VHM69_03530"
     CDS             <1..195
                     /locus_tag="VHM69_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2739495.1"
                     /translation="PFIMVSNTEMMTIPVGLATVQSSYGVRYADIMASAVLGGLPLII
                     VFLFFQRQIVQGIANTGLKS"
     gene            256..1269
                     /locus_tag="VHM69_03535"
     CDS             256..1269
                     /locus_tag="VHM69_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2739496.1"
                     /translation="MRDVASKAGVSFQTAGKVLNGKGTVSQETRERILDAAKALGYVP
                     NALARSLVTRSTCTIGIVASDLSDAELARFVVGAEREARRQGHGVLIGSIDREGTDAA
                     RYLRILLERRVDGILTAAPQMEHDARIGELLRGPVPAVSIHRIPGGGVPLVGSDHVET
                     ALLATRHLLSLGHRRIAAITGSEDRQVTSMRLKGYERALEEAGVTYESSLVESGNWEA
                     DGAFDATLRLLDRAPGITAFYAQNDTMALGVLSALHERGLGVPDDCAVVGCDDIPMAA
                     HAIPPLTTVHVPFYETGETAVRLLLEVIAGRMDRPKRILLPVRLVRRSSCGAGMEATD
                     RLS"
     gene            1285..>2026
                     /locus_tag="VHM69_03540"
     CDS             1285..>2026
                     /locus_tag="VHM69_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX2739497.1"
                     /translation="MAKIKVDLDRRIGDLDKRVFGGFVEHLGRCIYGGVFDEGSPLSD
                     ERGYRIDVLEAAKDLRIPILRWPGGNFVSGYHWTDGIGPREERPRKTELAWFTEESNR
                     FGTDEFIEYCRLLGTEPYICVNMGTGTMDEAQAWVEYCNGTGDTYWANLRRENGHEEP
                     FKVKYWGLGNEMYGEWQIGALSAEDYVRKAREFAKVMRWTDPNIELVGCGLSGLTDWD
                     RVVIEGLAPFVDYHSIHIYTGSDDYWSNV"
BASE COUNT          388 a          588 c          699 g          351 t
ORIGIN      
        1 cctttcatca tggtttcaaa cacggagatg atgacgatac cggtcgggct ggcgacggtg
       61 cagagctcat acggggtccg ctatgccgac atcatggcct ccgccgtgct cggcgggctg
      121 cccctgatca tcgtcttcct gttcttccag cgccagatcg tgcagggaat cgcgaacaca
      181 ggcctgaaga gctagtctga gtagtccagc gtacgggccg gtgttgaaag gagtgatcct
      241 ctgacgcacg tatcattgcg ggacgtcgcc tcgaaggcgg gggtgagctt ccagaccgca
      301 ggcaaggtgc tgaacggcaa ggggaccgta tcccaggaga cgagggagcg catcctcgac
      361 gccgcgaagg ccctcggata tgtcccgaac gcccttgcca ggagcctcgt gacccggagc
      421 acctgcacga taggcatagt agccagcgac ctgagcgacg ccgagctggc gcgtttcgtc
      481 gtcggcgccg agcgcgaggc gcgtcgccag ggccacgggg tcctgatcgg cagcatcgac
      541 cgggagggga ccgacgcggc gcgctacctg cgcattctgc tggagcggcg cgtcgacggc
      601 atcctgacgg cggccccgca gatggagcat gacgccagga taggggaact gctgcgcggt
      661 ccggttcctg cggtgagcat ccaccggatt cccggcggag gcgtcccgct cgtcggctcc
      721 gaccacgtcg agacagccct gctggcaacc cggcacctgc tcagcctggg gcaccggcgc
      781 atagcggcga tcacagggtc ggaggaccgt caggtcacct ccatgcggct gaagggctac
      841 gagcgtgccc tcgaagaggc gggcgttact tatgaatcat ccctcgtcga aagcggcaat
      901 tgggaggcgg acggggcgtt cgacgcgacg ctgcggctac tcgaccgggc acctggtatc
      961 accgccttct acgcccagaa cgacacgatg gcgctagggg tgttgagcgc gttgcacgag
     1021 cgggggctcg gggtgcctga tgattgcgcg gtcgtcgggt gcgacgatat tcctatggcc
     1081 gcgcacgcga tcccaccact taccacggtg cacgtgcctt tctacgagac gggagagacc
     1141 gccgtacgac tcctgctcga agtcatcgct ggcaggatgg acaggccgaa gcgcatactc
     1201 ttgccggtcc gactggtgcg ccgcagctcg tgtggagcag gtatggaagc tacagacagg
     1261 ctatcttaaa gggaggagtg aggggtggca aagatcaaag tcgacctcga ccgcaggatc
     1321 ggggatctgg acaagcgtgt gttcgggggg tttgtcgagc accttggccg ctgtatatac
     1381 ggcggcgtct tcgatgaggg ctctcccctg agcgatgaac ggggctacag gatagacgtt
     1441 ctcgaagccg cgaaggactt gcggataccc attctgcgct ggcccggtgg caacttcgtc
     1501 agcggctacc actggacgga tgggatcggc ccgcgcgagg agcgtccgag gaagacggag
     1561 ctcgcctggt ttacagaaga gtccaaccgc ttcggcaccg acgagttcat cgagtactgt
     1621 cgcctgctcg ggaccgaacc ctatatctgc gtgaacatgg gcactggcac gatggatgag
     1681 gcgcaggcct gggtcgagta ctgcaacggt acgggcgaca cctattgggc gaacctgagg
     1741 cgcgagaacg gccatgaaga gcctttcaaa gtcaagtact ggggtctcgg caacgagatg
     1801 tacggggaat ggcagatcgg ggctctctca gccgaggact acgtgaggaa ggcgcgcgag
     1861 ttcgcgaagg tgatgcggtg gacggacccg aatatagagc tcgtcggttg cggtctgagc
     1921 gggctcacgg attgggaccg cgtcgtgatc gaaggtctcg ccccgttcgt ggactatcac
     1981 agcattcaca tatatacggg cagcgacgat tactggtcga acgtcc
//
LOCUS       DATAIR010000145         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_50075, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000145 DATAIR010000000
VERSION     DATAIR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50075"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VHM69_03545"
     CDS             complement(<1..1053)
                     /locus_tag="VHM69_03545"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HEX2739498.1"
                     /translation="MPHVYVTGHKNPDTDTIASAIGYAEYKNLVDPENHYVSARLGEV
                     NTQTEWALKKSGAEAPNLLEHIMLRVKDVMSDDVAIANKNDPLRNVGLTMAKRNISQL
                     PIVEDDGSLTGIITERNLARMYVRESRGASSFDDTPVSVGAIVEVLEGELLVGEESCE
                     SSGQLWVISMSVASMGQSMKPGDIVVIGDRPKSQRRAIELGAEVLVVSNGVRPEEEVL
                     ELAREKGTTVVLSPLDSYVTSRLIQLAVPCWEVMSENPLTVNPDDLITDITEQVMEVH
                     YRAAIAVDDRYVPIGVITRTDLLNPKPRRVLLVDHAEVGQSVKGVEKAEVVEILDHHH
                     VGDIETTTPILATFDPV"
     gene            1164..1385
                     /locus_tag="VHM69_03550"
     CDS             1164..1385
                     /locus_tag="VHM69_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739499.1"
                     /translation="MPGNRSRRYDLLCIREDMGVPPDTHGGIFMSQGPVLLLGTHQIV
                     ASGMRGIAAFVACERLWVVSFLGKATILT"
     gene            1723..3075
                     /locus_tag="VHM69_03555"
     CDS             1723..3075
                     /locus_tag="VHM69_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739500.1"
                     /translation="MLHEALVPHADLSARELVVRLALDGEEHRASFSCQQGFGSASDS
                     LAAFTLLAAMKAGGSLELPEPLSPRLLSTMPRIQDIYHAWDRGRFKRVAVRARPRRGQ
                     ATRASGVGCLFSGGLDSFYTLLKHRDEVTHVIFADGYDIPLQDTARRERAVNAARTVA
                     DELGKSLIEVHTDLQIFTRDVGLMWLFYHGAALAALALLFQDRLGRVLIPASFSYADL
                     FPWGSHPMLDPLWSTEQTAIEHSGCDVTRVDKAGYVSAFPVAMRNLRVCSQHRADYNC
                     GHCEKCLRTMINLRAAGASERCETLPEVPDPETIANLSLQGENGRAYALENLKALERL
                     GTEPELARALDTALENSFGGEHLRRRLDETRAELLETRRRAHKGVTKHRERAERFKSL
                     AEELEMDNKRLAEANAHLKGHYSSRRYRLVDLLANGALKMPGLKRLLRKAATLTGASR
                     "
BASE COUNT          636 a         1076 c         1021 g          639 t
ORIGIN      
        1 gacggggtcg aaggtggcaa ggatgggagt ggtcgtctcg atgtctccga cgtggtggtg
       61 gtccaggatc tcgacgacct cagccttctc gacgcccttg acgctctgcc cgacctcggc
      121 gtggtctacg aggagcacgc gccgcggctt cgggttcaaa agatccgtcc tggtgatcac
      181 cccgataggc acgtatctgt cgtccacggc tatggccgcc ctgtagtgga cctccatcac
      241 ctgctcggtg atatccgtga tgaggtcgtc ggggttgacg gtcagagggt tctcgctcat
      301 gacctcccag caggggacgg cgagctgtat caggcgactc gtcacgtagg agtcaagcgg
      361 actgagcacg accgtcgtcc ccttctccct tgccagctca aggacctctt cctccggacg
      421 caccccgttg gagacgacga gcacttccgc gccgagctct atggccctcc tctggctctt
      481 cgggcggtcg ccgatcacca cgatatcccc gggcttcatg gactgcccca tcgaagccac
      541 gctcatcgag atcacccaca actggcctga ggactcacag ctttcctccc cgaccagcag
      601 ctcgccttca agcacctcga ctatcgcccc gaccgagacc ggagtatcgt cgaagctgga
      661 ggccccgcgc gactcccgca cgtacatacg cgccaggttc cgctccgtta tgatccccgt
      721 gagcgatccg tcgtcttcca cgatggggag ctggcttatg ttccgcttgg ccatcgtcag
      781 gccaacgttg cgtaagggat cgttcttgtt ggctatcgcc acgtcatcgg acataacgtc
      841 ctttacgcgc agcatgatgt gctcgagcag attcggcgcc tcggccccgc tcttcttcaa
      901 agcccactcg gtctgggtgt tgacctcacc gagccgggcc gacacgtagt ggttctccgg
      961 atcgacgagg ttcttgtact cggcgtatcc gatcgccgag gcgatagtgt ccgtatcagg
     1021 gtttttgtga cctgttacgt atacgtgtgg caaggtgtcc tccatccccc tttgtctgaa
     1081 ctggtcacat acggaagtcc catattactg acgcccacct gacggcaacg cgggtgctca
     1141 ggtgccgaga aaagtctacc agaatgcctg gaaaccgtag ccgccgctac gacctgctgt
     1201 gcatccgaga ggacatgggc gtacctccgg atactcatgg cggaatcttt atgtcccagg
     1261 gaccggtgct ccttctcggc acgcatcaga tcgtagcgtc aggtatgcgc ggcatcgcag
     1321 ctttcgtcgc ctgcgagcgc ctatgggtcg tctccttcct gggcaaggcg accatcctca
     1381 cgtaacgggc gcacgaggag gacctgcctc ccgaggggcg gcgctactcc ctttcactgc
     1441 acgacaccgg cgaacgggcg gtgcagtgag ggtcattacg agcacgcggc gggaacgcag
     1501 ccgggctaag gtacgccggg cttgctgacc tcgaagcaga ggaacccggg cgtccgggag
     1561 agcttctcgt acgcttcggg atcgcctggt gagcgtatgg atcaggagat cagacaccag
     1621 gccggagggg tgatctccac acgaacctgt gccgtacggt ctttaacctt accccgcgat
     1681 ggtgctacgc ttaccgtttt cgagtaccag ggaggtcagg acttgcttca cgaagcgctt
     1741 gtgccacacg ctgatctgtc agcgcgagag ttggtcgtgc gcctcgcgct ggatggcgag
     1801 gagcaccgcg cttcgttttc gtgccagcag ggcttcggtt ccgcgtcgga ctctctggcg
     1861 gcttttaccc tgttggcggc gatgaaggcg ggaggatctc tggagctccc cgagccgctc
     1921 tcgcccaggc tcctttcgac catgccccgg atccaggaca tctaccacgc ctgggatcgg
     1981 ggcaggttca aacgcgtcgc agtgcgggcc aggccacggc gagggcaggc gacgcgggcc
     2041 tccggcgtcg gctgcctctt cagcggcggg ctcgactcct tctacacgct tctcaagcac
     2101 cgcgatgagg ttacgcacgt gatcttcgcg gacggctacg acattccgct gcaagacacg
     2161 gcgcgcaggg agcgggccgt caatgcggca aggacggtgg ctgacgagct aggcaagtcc
     2221 ctgatcgagg tccacaccga ccttcaaatc tttacccgcg atgtcgggct gatgtggctt
     2281 ttctaccacg gcgccgcgct cgccgcgctc gccctgctct tccaggaccg cctcgggagg
     2341 gttctgatcc cggccagctt ctcgtatgcc gacctgttcc cgtggggctc gcatcctatg
     2401 ctcgatcccc tctggagtac ggaacagacc gccatcgaac actccggctg tgacgtgacg
     2461 cgtgtagata aggccggcta cgtctccgcc tttcctgtcg ccatgaggaa cctgcgcgtc
     2521 tgttcccaac accgcgccga ctataactgc gggcactgcg agaagtgcct gcgaaccatg
     2581 ataaacctgc gcgccgcagg ggcttccgaa agatgcgaga cgctgccgga agtacctgac
     2641 ccggagacca tcgccaactt gtcactgcaa ggggagaacg gcagggccta cgcgctcgaa
     2701 aacttgaagg cactcgagag gcttgggacc gaacccgagc tcgcccgcgc cctggatacc
     2761 gccctcgaga acagcttcgg aggagaacat ctacgcagaa ggctcgatga gacccgcgca
     2821 gagcttcttg agacaaggag aagggcgcat aaaggggtca cgaagcacag agagcgcgcc
     2881 gagcgcttca agtcactggc cgaagagctg gagatggaca acaagcgcct cgccgaggcc
     2941 aacgcgcacc tgaagggtca ttactccagc cggcgctacc ggctcgtgga cctcctcgcc
     3001 aacggcgctc tcaagatgcc agggcttaaa cgtctgctcc gcaaggccgc gacactgact
     3061 ggcgcatcac gatagcattc aggctttgat gaggtgacta tccgacctgt gtataaggcc
     3121 gattgctaca atgatgtcgg tgggtacaca agcgtcccga gaggaacacc tgcatcggag
     3181 catggaaggt aaagccgccg gcttcgtgca aaacgtgagg ccggccaacc gaaccgagat
     3241 acggtgagcc ccggtagaag acaccagtct caaggtactc ctcagcataa cctacctgcc
     3301 ccaaggagca gcttcgtagg ccgtgagcgc gagatgctgg aggtcaagca agcgctgacc
     3361 acgacacggc tg
//
LOCUS       DATAIR010000146         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_424215, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000146 DATAIR010000000
VERSION     DATAIR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424215"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHM69_03560"
     CDS             <1..592
                     /locus_tag="VHM69_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX2739501.1"
                     /translation="NTATAAALRATQRSFEVPVIGVIEPGARAAVEETLNRRVGVLAT
                     PLTAASGAYRRAVHSHDAGVEVHEQACPEFVPLIERGVVEGPELERGARGYLEPLMDR
                     RVDAVVLGCTHYPLISAPINRILGPEVRLISSAGQTALEVGRVLSRRDRLRRPRHIDD
                     EGEATYVCSADPEEFAALGSRFLDEEIKAVAPATVI"
     gene            631..704
                     /locus_tag="VHM69_03565"
     tRNA            631..704
                     /locus_tag="VHM69_03565"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:665..667,aa:Pro,seq:tgg)
     gene            749..819
                     /locus_tag="VHM69_03570"
     tRNA            749..819
                     /locus_tag="VHM69_03570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:781..783,aa:Gly,seq:tcc)
     gene            844..916
                     /locus_tag="VHM69_03575"
     tRNA            844..916
                     /locus_tag="VHM69_03575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:877..879,aa:Arg,seq:tct)
     gene            complement(1073..1291)
                     /locus_tag="VHM69_03580"
     CDS             complement(1073..1291)
                     /locus_tag="VHM69_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739502.1"
                     /translation="MASSFEKSYPNITRWVTSQGWIELGQDEYSSSLVRVLDEGGMIW
                     EGSTYYGTLDEAFDALETALAEWMEQGA"
     gene            1409..1660
                     /locus_tag="VHM69_03585"
     CDS             1409..1660
                     /locus_tag="VHM69_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739503.1"
                     /translation="MRDHNYTTEEIIRRGHEIYDRDIHDRLEASHEGQFVVVDVTTGT
                     WEIDEDDVAASDRALARNPDAVLYFARVGHPAAYRLGVR"
     gene            1686..2261
                     /locus_tag="VHM69_03590"
     CDS             1686..2261
                     /locus_tag="VHM69_03590"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clan AA aspartic protease"
                     /protein_id="HEX2739504.1"
                     /translation="MRGTVTSDGREAVLRVEVLSARGTSSLRVETVVDTGFTGHLTLP
                     SATVEALGLPTIGSAESILADGSMVMEDVCMARVLWHGEERPVRVLVSDATPLLGMAL
                     LRGSELRIECLGGGEVSIEKLEEPGGQRNRETIPSFLRRDLYHVPIRVARSHLRAVFP
                     LLPGRHTDHQILDHAGSGIGGSPDHSGAFGR"
     gene            complement(2348..>2610)
                     /locus_tag="VHM69_03595"
     CDS             complement(2348..>2610)
                     /locus_tag="VHM69_03595"
                     /inference="COORDINATES: protein motif:HMM:NF022970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739505.1"
                     /translation="ERERAIGWYYVEGNLNFPFLARCAARRSISHLKVGDEVEVIGMA
                     PVEECEHEMFVEMDWDEEELAVPLSQLEVISADDETREAVED"
BASE COUNT          499 a          824 c          798 g          489 t
ORIGIN      
        1 caacacagcg acggctgccg ccctgcgggc gacgcaacgc tccttcgagg tacccgtgat
       61 aggcgtcata gagccgggcg ccagggccgc ggtcgaggag acgctcaaca ggcgcgttgg
      121 cgtccttgcg acgccgctga ccgcggccag cggggcatac aggcgtgccg tgcactccca
      181 tgacgccggg gttgaggtcc acgaacaggc atgtcccgag ttcgtgccgc tcatcgaacg
      241 cggcgtcgtc gaaggtcccg agttagaaag gggcgcgcga ggctatctgg aacctctcat
      301 ggatcgcagg gtcgacgcgg tggtgctcgg gtgcacccac tatccgctca tctcagcgcc
      361 gatcaaccgc attttggggc ctgaggtgcg cctgatctcc tccgcaggcc agacagcact
      421 tgaagttggc cgcgtcctct cccgccgcga ccgcctccgt cgcccccgcc acatcgacga
      481 cgagggcgag gccacctacg tgtgcagcgc cgaccccgaa gagttcgccg ccctcggaag
      541 ccgcttcctc gacgaggaaa taaaggccgt cgccccggcg accgtaattt aaacacgaac
      601 acccctttgt tatactcatg cccggtctcg cggggcgtgg cgcagcctgg tagcgcacct
      661 gctttgggag cagggggtcg gaggttcaaa tcctctcgcc ccgacagcga agctggcgag
      721 gccacgcgta cttcggccag gccgccgcgc gggtgtagct caacggtaga gccccagcct
      781 tccaagctgg tgatgggggt tcgattcccc tcacccgctc tctcgcgcca accggcgcga
      841 agggcgcccg tagctcaggg gacagagcag cggacttcta atccgccggt cgcaggttcg
      901 aatcctgccg ggcgcgcatc taaaagtcct gcaaaacgcg gaaaatcgaa aggaccgaaa
      961 ctacctgttc tcggccctgt ggcagcagta gggcagcagt agacgaaaaa ttcgccccgg
     1021 atgtacggga aaatcgtggc cgagcaggac cgggcgaggc acgcgtttct catcaagcgc
     1081 cttgttccat ccactccgct agagccgtct ccaaagcgtc aaaggcttcg tccagggttc
     1141 cgtagtaagt gctgccttcc caaatcatgc cgccttcatc cagcaccctt accagcgaag
     1201 aactgtattc gtcctgcccc agttctatcc aaccttgcga tgtcacccag cgggtgatgt
     1261 tcggatagct tttctcgaat gagctcgcca cttgcctcat cctctcgcat caagaccttc
     1321 gcctccggtc gccacggaac tgctaatcgg tgcccattga cactaacaag ttaacccgga
     1381 agcgtcctcg gacgctagaa tgtagcgcat gagagaccac aactacacca ccgaggagat
     1441 aatccggcgc gggcacgaga tatacgatcg cgatatccat gaccgcctgg aggcttccca
     1501 cgagggtcag ttcgtagtcg tggacgtgac aacggggacg tgggagatcg acgaagacga
     1561 cgtggccgca tcggataggg cgctcgccag gaatccggac gccgtgctct atttcgccag
     1621 ggtaggccat cccgcggcat accgcctggg cgtccgctag agtcttcgtt ggcggcactc
     1681 tggcggtgag gggtacggtc acctccgacg gccgcgaggc agttctgcgc gtcgaggtcc
     1741 tgtctgcgcg tggcacctct tcgctccggg tagagacggt ggtggacacc ggcttcaccg
     1801 gccacctcac gctcccatcc gcgacggtcg aggccctggg tctgccgacc atcggaagcg
     1861 cggagtcgat tctcgcggac ggtagtatgg tgatggaaga cgtttgtatg gcgagggtgc
     1921 tctggcacgg tgaggagcga ccggtacggg ttctcgtgtc cgacgcgacg ccgctgctgg
     1981 gcatggcgct tttgcgtggc agcgaactgc gtatcgaatg cctgggcggt ggggaggtta
     2041 gcatagagaa gctagaggag ccgggtggcc agcgaaatag ggaaacgatc ccgagctttc
     2101 tcagaagaga tctctaccat gttcccatca gggtggcgag gtcccatctg cgagcggttt
     2161 tcccactact gcctggccga catacagatc atcagatcct ggaccacgcc ggctccggca
     2221 tcggcggaag ccctgatcac tctggagctt tcggcagata gacaaaggcc gcggtgtcgg
     2281 tgatgctaag gagattcggc agcgcaacgt tcgctcagaa ctggtagccc ttcttggtcc
     2341 agtagagcta gtcctcgaca gcctccctgg tttcgtcatc ggcagagatc acctcgagct
     2401 gggagagagg aactgccagt tcttcctcgt cccagtccat ctcgacgaac atctcgtgtt
     2461 cgcattcctc cacgggagcc atgccgatca cctcgacctc atctcctact ttcaggtggg
     2521 agattgatct tctggcagcg caccgggcga ggaaagggaa gttcaaattc ccttcgacgt
     2581 agtaccaccc tatggcccgt tctcgctcat
//
LOCUS       DATAIR010000147         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_424223, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000147 DATAIR010000000
VERSION     DATAIR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424223"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VHM69_03600"
     CDS             complement(<1..237)
                     /locus_tag="VHM69_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739506.1"
                     /translation="MSEGRNAPEVFRRDALRVAFIGVASAALYLVVYLTQWAMFRNGL
                     RQEVAGTLVLGEPADGTWMILQSGGYYGATLLLFL"
     gene            complement(524..1315)
                     /locus_tag="VHM69_03605"
     CDS             complement(524..1315)
                     /locus_tag="VHM69_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739507.1"
                     /translation="MMSEAGERQVERGQKDGGAVMKRIFSKAIMAGRTTALTLGVAVM
                     LALVLGIGTTAVAATGANFLLGKANSARAVSKLTSAVAGPSLQLVNSGTAAAATALNL
                     KVPAGHAPLTVNAEAGTATNLSADRLDGKDSNDFQQANATASGDLSGTYPNPQLDQGA
                     VGSDEVANWSLGIEDVTKSAGFASLDPGVVPASGCIDQSVMGMDVAQWDFILVNPPRS
                     LPEGLVASPMLASATGPFTFRICNMKSSSIDPPAGVWGIAAFDAN"
     gene            complement(1787..>2184)
                     /locus_tag="VHM69_03610"
     CDS             complement(1787..>2184)
                     /locus_tag="VHM69_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739508.1"
                     /translation="GDTLASSQGNDRLYGDSNPSGAKDYVYDWEGRNVLSGGPGDDHL
                     LGHNRLSGGSGDDTIRGSRPNGTERVRTMSGGPGTDRITSEGFVKDTIYAQDGEKDNI
                     NCGGGFDIVYFDEGIDFASTKTCENRIGN"
BASE COUNT          389 a          683 c          671 g          441 t
ORIGIN      
        1 taggaagagg agtagtgttg ccccgtaata gccgccgctc tggaggatca tccatgtgcc
       61 gtcggcgggt tcccccagaa cgagcgtgcc ggcgacctcc tggcgcagcc cgttgcggaa
      121 catcgcccac tgcgtgaggt acacgaccag gtacaacgcg gcgctggcca cgccgataaa
      181 agctacccgg agcgcgtcgc gccggaagac ttccggcgcg ttcctcccct cgctcactgg
      241 tggtcgagca acggggaggc tcgcacgctg accttagttg ccgcaaagtt agccatcagc
      301 ggcagactag cgtattgcca gtctgacgga aagaccggct ggatgcgctg gcaccggcag
      361 acctgcaatg tcatgctcca catatcctcg ccttccatgc aacacgcgaa gcgcccaata
      421 gagcccggtt cgcgtatcgt ctgcatccgt caccggcgta tctctccacg gagagatacg
      481 ccggcctctg aggcgtctac aggagagttc ccgtggataa gagtcagtta gcgtcgaagg
      541 cggcgatacc ccagacaccc gccggtggat ctatgctgga actcttcatg ttgcagatcc
      601 ggaacgtgaa cggccccgta gcgcttgcca gcatggggga tgccacgagt ccttccggca
      661 acgaacgcgg ggggttcacg aggatgaaat cccactgggc gacgtccatg cccattacgg
      721 attgatctat gcaccccgag gcgggcacga cgccaggatc gaggctggca aaacctgcgg
      781 acttggtcac gtcttcgatg ccgagcgacc agttggctac ctcgtcggat cccaccgcac
      841 cctggtcgag ctgcgggttg ggataggttc cggaaaggtc tccactcgcc gtggcattcg
      901 cctgctggaa gtcgttcgag tccttgccgt ccagcctgtc ggcagagagg ttggtggccg
      961 tgccggcctc ggcgttcacc gtgaggggag cgtgaccggc ggggactttg aggttcaggg
     1021 cggtggctgc cgcggcggtg ccactattga cgagttgcag gctcggtcct gctactgcgg
     1081 aggtcagttt gcttacggca cgtgccgagt tggccttacc gagcagaaaa tttgcccccg
     1141 tggccgcgac cgccgtcgtg ccgattccga gcaccagggc cagcatcacc gcgacaccca
     1201 gggtcagcgc ggtggtcctt cctgccatta tcgctttcga gaagatccgc ttcatcaccg
     1261 cgcctccgtc cttctgtcct cgttcgacct gccgttcgcc agcttcactc atcattcgtt
     1321 tgtgctcctt ccctttctgc ttctactccg tcatgctctg gtccgtagtc tgtaaccaag
     1381 ccaggcggcg aaccatccac agcacaccca cagaacaccc acaccgtccc cacacagccg
     1441 gttgtgggag cgaggcgagg agtagacggc gtgcggggac aaccccgatg ccgcagtgct
     1501 agacgcccgc ggaggcgagt tcagggtcac ggaaactgcc gatatccagg atcagacccg
     1561 gcgcttctga gcgccgacgg gcgtactgta cgctcacctt tcaagaacgg ctgagggtta
     1621 ttgaggccct tcttgtaacg agcttgacgg cgctgccttc cgaggtgccg acttcgacgc
     1681 cctgatgagc gagcaaggaa ctacgttcgg gttcatgctg ggagaagacg agatcgtcta
     1741 tggggtgtga gaataaccag gccctgagtc gcctcggaga agggagctag ttgccgatgc
     1801 ggttctcgca ggtcttggtg ctggcgaagt ctatcccctc atcgaagtag acgatgtcga
     1861 agccaccgcc gcagttgatg ttatctttct ccccatcttg tgcgtagatg gtgtccttga
     1921 caaagccctc cgaagtaatc cggtcggttc cggggcctcc actcatggtc ctgactctct
     1981 cagtgccgtt ggggcggctt cctctgatcg tgtcgtcgcc ggagcctccg ctgagcctgt
     2041 tgtgtccgag gaggtgatcg tcgccgggac ctccagagag cacgtttcgc ccctcccaat
     2101 cgtagacgta gtctttcgcg ccgctgggat tcgagtcgcc gtagagcctg tcgttgccct
     2161 ggctagaggc aagggtgtcg ccac
//
LOCUS       DATAIR010000148         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_8978, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000148 DATAIR010000000
VERSION     DATAIR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8978"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..243)
                     /locus_tag="VHM69_03615"
     tRNA            complement(171..243)
                     /locus_tag="VHM69_03615"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Ala,seq:tgc)
     gene            654..896
                     /locus_tag="VHM69_03620"
     CDS             654..896
                     /locus_tag="VHM69_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2739509.1"
                     /translation="MALYEYKCAGCEDHFDLMRPMSAADEPAECPECGSTDSRRVISN
                     FASITPGASALSTNPVMDARMAGGGGGCCGGGCGCG"
     gene            856..1302
                     /locus_tag="VHM69_03625"
     CDS             856..1302
                     /locus_tag="VHM69_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX2739510.1"
                     /translation="MAAVAVAGAAAAARGSVTGEAPEGSPEGLPDLALKGIEEFNRGE
                     FYECHEYLEEAWMQEPRRVRFLYQGILQVGVGFYHQQNGNWRGATGLLRNGTQRLKEF
                     EPETLGIDVARLVRESERCLRELEELGRERVGEFDQSRVPKVEWTK"
     gene            complement(1529..2125)
                     /locus_tag="VHM69_03630"
     CDS             complement(1529..2125)
                     /locus_tag="VHM69_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2739511.1"
                     /translation="MDEILSLIEHYGYLFVFFGVMLGCSGIPFPGATILLAAGILVQQ
                     GHLELGYAIAFGILGAILGSQIGYWAGQRGGRSFVLKWGRYVKITPERLERVERFFVL
                     YGGKAVFASRFVSVFRVLGAPVAGISRMRGSTFFLYSALGGMVWTTVVVLLGYFFGQS
                     WAGAQHWTGRGPLLLALLLAVALGLYVAYRWVLRYRRS"
     gene            complement(2462..>3087)
                     /locus_tag="VHM69_03635"
     CDS             complement(2462..>3087)
                     /locus_tag="VHM69_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX2739512.1"
                     /translation="IRDQIDEILQMYRRTLQDDRLCLLDRYRFVDVARKVVGVGSVGT
                     RDYVVLFEGRDEDDPLILQVKEAEASVLEGHLPQSTYEHQGQRVVAGQRLMQAASDIF
                     LGWLRGPGGRDFYWRQLRDMKGSAKIERMSPDELAIYASFCGWALARAHARSGDRVQI
                     SAYLGKSERFDGAIVDFAVSYADQIERDHAALCAAVKSGKIAGDVVA"
BASE COUNT          639 a          914 c          941 g          593 t
ORIGIN      
        1 gcgaaagtca gaagcctccg ccggcggcgg ctcctagggt attctatctc catatactgg
       61 aaagcaaact tcctgcgttg gtgtcaggtt ggtgtcaaaa tcagttcgac gaaatatggg
      121 cagaaccaac tgctcggcgg ttttacctgc aaatcgccaa ctttttgaag tggagccgag
      181 gggattcgaa cccctgacct ccgccgtgca aaggcggcgc tctcccaact gagctacggc
      241 cccagaccta gcctaggggg cgagtttacc gcagttacgc gaataggact aacgagggtc
      301 gagtgtgtct gctgcgccgg atctcatgct ctcggaagtc aacttcatca cacgagatgg
      361 tgcgcctggc cttgacacat tggctatgtg cgatatgaaa atagtagccg gtgtccacgc
      421 ttctcctttc cctttcccca ttacccctga aaggcgggca ccggcttcga gcttatcatt
      481 ccccttcgtc gatgccgccg tcctttcagc agacttggcc gaaccttaat gccgggtaag
      541 gtccctgacc tctccgtcaa ccgatgatcc tcgtacgggc gggctccttc agggcgcgac
      601 agactgtaca atacctttgc cagaaaataa tcggaccgag aaggtgggag aagatggccc
      661 tctacgagta caaatgcgca ggctgtgaag atcacttcga cctcatgcgc ccgatgagcg
      721 ccgcggacga gcccgcggag tgtcccgagt gcggctcgac cgactcgcgg cgtgtgatct
      781 ccaacttcgc ctccataact ccgggcgcct cggccctctc gacgaaccct gtgatggatg
      841 cccgcatggc gggtggtggc ggcggttgct gtggcggggg ctgcggctgc ggctagggga
      901 agtgtgacag gagaggcgcc ggagggatct cccgaagggc ttcccgacct cgccctcaag
      961 ggtattgaag agttcaacag gggcgagttc tacgagtgcc acgagtacct tgaagaggcc
     1021 tggatgcaag agcccagacg cgtccgcttc ctctaccagg gcatcctcca ggtcggcgtg
     1081 ggcttctacc accagcagaa tggcaactgg cgcggggcca cgggcctgct gcgcaacggc
     1141 acacaacgcc tcaaggagtt cgagccagag accctgggta tagacgtggc gaggctcgtc
     1201 cgcgagagtg agcgttgcct cagagagttg gaagagctgg ggcgtgagag ggtcggagag
     1261 ttcgaccaat ctagggtgcc gaaggtggag tggacgaagt agacggcccg gagcgcgtgc
     1321 gatccgtttg aatcggtgcg tctggtagga ataatatggc ctgatgcgcg gggacgtagg
     1381 tgggcatatg agacaggaat atagtctctc gctgtactgt atccacagct cacggatctt
     1441 ccggctcgca atatctgcgt tcgtagccgt tgcgagaacg cgaacatcgc gcggatggat
     1501 aactggcacc gtcgaagaag cgcaagacct atgacctcct gtagcgaagc acccaccgat
     1561 aggcaacata aaggcctagc gccacagcga gtagtagagc cagcaagagg gggccccgtc
     1621 ctgtccaatg ctgcgccccg gcccagcttt gaccgaagaa gtaccccaac aagacgacga
     1681 ccgtagtcca caccatcccg ccgagagcgc tgtagaggaa gaacgtgctt ccgcgcatgc
     1741 ggcttatacc tgcaaccggc gctccgagca cgcggaagac cgagacgaag cgggacgcga
     1801 agacggcttt gccgccgtag agtacgaaga accgttcgac tcgctccagg cgctctgggg
     1861 tgatcttcac atagcgcccc catttcaaga cgaacgatcg tccccctcgc tggcctgccc
     1921 agtaacctat ctggctgccg aggatggccc caaggatgcc aaatgctatg gcgtatccga
     1981 gttccaggtg gccctgctga accagaatgc cggcggccag gaggatggtt gcgccaggga
     2041 atgggatgcc cgaacaccct agcatcacgc cgaaaaacac gaacaggtaa ccgtagtgct
     2101 cgatcaggga gaggatctca tccattgcag cagattatag tggtggcagc cgcctcaggg
     2161 atgccgccgg gacatgggca gatccccaag tggacctttg ttttggggcg atcaggtgac
     2221 gaggcgccgc tatgggttgc cggcttccgc cgagtcatag tgtcgagaca agaatgacct
     2281 ctgtgccacg ggcaggcatg tggatcaggg acctcagaaa ggttgatttg cgacgggcac
     2341 gagcgtctgc gagtgcggaa agatttgcta tgtccttctc gtacaccttc gtaccttctc
     2401 cgagcgtttc gcatcgttga ggaggcggct ccgctctggt tcaaagggtc tgatgaactc
     2461 ctcaagcaac tacgtctccc gcgatcttac ccgacttcac ggcagcgcac agtgcagcat
     2521 ggtcgcgttc gatctggtct gcgtaggaca ccgcgaagtc gacgatggct ccatcgaagc
     2581 gctcgctctt gccgaggtag gccgagatct gcacgcggtc gccggagcgg gcgtgcgcgc
     2641 gggccagcgc ccagccgcag aagcttgcat agattgccag ttcgtcgggc gacatgcgct
     2701 ctatcttcgc cgagcccttc atgtctcgca gttgacgcca gtagaagtcg cggccgcctg
     2761 gtcctcggag ccagccgagg aagatatcgc tcgcagcctg catcagccgc tgccccgcca
     2821 cgacgcgctg cccctgatgc tcgtaggtac tttgaggaag atggccctcc agtaccgacg
     2881 cttccgcctc tttgacctgg aggatgagtg ggtcgtcttc atcgcggcct tcgaacaata
     2941 cgacgtaatc cctggtgccc acgctgccaa ccccaacgac ctttcgcgcg acgtccacga
     3001 aacggtatct gtcgagcagg caaagacggt cgtcttgcag ggtgcgccta tacatctgca
     3061 gaatctcgtc gatctgatcc ctgatct
//
LOCUS       DATAIR010000149         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_101324, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000149 DATAIR010000000
VERSION     DATAIR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101324"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VHM69_03640"
     CDS             complement(<1..1285)
                     /locus_tag="VHM69_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX2739513.1"
                     /translation="MTPITRRQFLRRTGKAALAVTVLSQAEFLGGCGDTTIWNELAQK
                     LRGRLVRPGDSYYGPLHLPFNRRYADVRPQGIASCLDPSDVRESILWAREHGMPVAIR
                     SGGHNYAGYSTTDGLLIDLSRMRRIVVDDGTGIATVQAGARNTGVYAGLQPHEAAISA
                     GRCPTVAIGGLVLGGGIGFSSRKLGLTCDSLRETVIVTADGKILTCNERENEDLFWAC
                     RGGSGGNFGVNVSYTFQSTPVKDVSIYDLQWDLKDGEKVLPALQDVVLQAPDDFSCRM
                     GGGRSGKDGGGRSPANVNALGQYFGPREELLEILEPVLRLAKPTKSVIEDKTFWQAKD
                     YFFHNTPTDRFQVKSAFVQKTLPEQGIEELLRYVDRWPGSSNEDGAGFAMFALGGAIS
                     RIPEEKTAYAHRDSRFLLAIDSSWASSDSHRTEQEN"
     gene            1632..>2382
                     /locus_tag="VHM69_03645"
     CDS             1632..>2382
                     /locus_tag="VHM69_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX2739514.1"
                     /translation="MVSGKDLILRGWPQGRTIGLALAAAERLRSRGMDDDAILRELEK
                     VRAAPYSTPDEALVPLARELASLRDEESAALQDRLRDEALAYGVWGQDLIEAGTTAQM
                     EGAMRLPVSVGGALMPDAHLGYGLPVGGVLATEGAVIPWAVGVDIAPVRGDTKIPTLD
                     GRSYPVKDLCEKGEEFGVWSCKPDGKVVAAKARAHQTQRDAPLVRITLNNGEAVDCTP
                     EHRFMLRDGSYLEADRLSPGASLMPFYTSVDR"
BASE COUNT          443 a          727 c          757 g          455 t
ORIGIN      
        1 cgttctcctg ctcggtgcgg tgcgaatcgc tgctggccca ggagctgtcg atggccagaa
       61 ggaaccgcga gtcgcgatgc gcgtaggcgg tcttctcttc ggggatgcgt gagatggcac
      121 cgccgagggc gaacatggca aagcctgcgc cgtcctcgtt cgagcttccc ggccagcggt
      181 caacgtaccg gagtagctct tcgatcccct gctcgggaag tgtcttctgg acgaaggccg
      241 acttgacctg gaagcgatcg gttggggtgt tgtggaagaa atagtccttg gcctgccaga
      301 aggtcttgtc ttcgatgaca ctcttcgttg gcttcgccag gcgcagtacc ggctccaata
      361 tctcgagaag ttcctcgcgc gggccgaagt actggccgag ggcgttgacg ttcgccggac
      421 tacgaccgcc gccgtctttc cccgaacggc cgccgcccat gcggcaagag aagtcatcgg
      481 gagcttgcag gacgacatcc tgcagcgcag gtagcacctt ctctccgtct ttgaggtccc
      541 attggagatc gtagatcgac acgtccttga ccggagtcga ctggaaagta taggagacgt
      601 tgacgccgaa gttgcctccg ctccctcccc ggcaggccca gaacagatcc tcgttctctc
      661 tctcgttgca ggtgagtatc ttcccgtcgg ccgtgacgat cacggtctcc ctcaagctat
      721 cgcatgtcag tccgagcttg cgggaactga agccgatccc accccccaga accagaccgc
      781 cgatcgcgac cgtcggacaa cgccccgcgg agatggccgc ctcgtgcggc tggagccctg
      841 cgtagacgcc ggtattgcgt gcgcccgcct gcacggttgc aatccccgta ccgtcgtcga
      901 cgacaatgcg cctcatgcgg ctgaggtcga tcaggagtcc atcagtggtc gagtacccgg
      961 cgtagttgtg tcctcccgag cgtatggcga ccggcatgcc gtgctcgcgc gcccacagta
     1021 tcgactcgcg cacgtcggaa gggtcgagac aggaagcgat cccttgaggg cgcacgtccg
     1081 cgtagcggcg gttgaaggga agatgcagcg ggccatagta ggagtcgccg ggacgtacaa
     1141 gacggcctcg aagcttctgg gcaagctcat tccagatggt ggtatcccca cacccgccca
     1201 ggaactcggc ctgagagaga acggtgacgg ccagggctgc cttccccgtg cggcgaagga
     1261 attgtctgcg cgtaatgggg gtcatggcca ggccgaacta gcggatttat tcatctatga
     1321 agcttccctt tcgattggtt tatggttgtc cctcaagaga ggtccccggc attgcgtccc
     1381 cgcctcacaa cggactcacc tgttcgcccg ccgcaatgac tgtagcaggt gaaaagacgc
     1441 gccgcaccac ccgatagagg gactcttgct gtcaatctca ctaccgtccc gtatttccat
     1501 agagaaggga atcctggctg cccggcgggg cgatctcgtc cgaagtcaca cgcacgaagc
     1561 gcgcttcttc tctttcgggg gtcagtcccg cgtgctcgga cggtgtataa ctttcgaagg
     1621 tgagaagaga gatggtgagc gggaaggacc tgatcttacg cggttggccg cagggacgca
     1681 cgatcggtct cgccctcgcc gcagccgaga gacttcgttc gaggggaatg gacgacgacg
     1741 caattctccg cgagctggag aaggtcaggg cggccccata ctccaccccc gacgaggccc
     1801 ttgtacctct cgcccgagaa ctggcaagtc ttcgggacga ggagagcgcc gcgctccaag
     1861 acaggctgcg cgacgaggct ctggcttacg gcgtgtgggg ccaggatctt atcgaggcgg
     1921 gcacgaccgc ccagatggag ggcgctatgc ggctccccgt ctccgtcggt ggcgccctta
     1981 tgccggacgc ccacctcggc tacggactac ccgtcggcgg cgtcctcgct acggagggcg
     2041 cggtcatccc atgggccgtg ggggtggaca tcgctcctgt gcgaggagac acgaagattc
     2101 cgaccctcga tggccggagc tacccagtga aggatctctg cgagaagggc gaggaatttg
     2161 gtgtctggtc gtgcaagccc gatggcaagg tggtcgctgc caaagccagg gctcatcaaa
     2221 cgcagagaga tgctcctcta gtaaggatta cgctgaataa tggagaggcc gtagactgca
     2281 cacccgaaca tcgctttatg ctgagggacg gttcctacct ggaagctgac aggctttcac
     2341 cgggtgcctc gcttatgcct ttctacacca gcgtggatag gg
//
LOCUS       DATAIR010000150         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_424305, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000150 DATAIR010000000
VERSION     DATAIR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424305"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VHM69_03650"
     CDS             <1..424
                     /locus_tag="VHM69_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739515.1"
                     /translation="GFSSTSYLVSVQNAVPWRRRGVATSSVVFFRTVGGSLGVAVMGA
                     LLNASLGERYRAAVDKAAGGDAGLERLLSDPNALLQPAVRSRIPEAVYSELAGALAAS
                     LSPAFWVLLFFGLSALAVATLFPRGTAKDLVSREEDYA"
     gene            417..1064
                     /locus_tag="VHM69_03655"
     CDS             417..1064
                     /locus_tag="VHM69_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739516.1"
                     /translation="MRETGRELTLDDLPRPPLFKLVDPEGRDVFQQTEVGGESARVGV
                     LFSDRELAGEFSAGASAHGMQDLSGLDPREVSDWGSVEDFALSGADFVLVVSEIGAGL
                     FYAGDLAHVAQEVSGEIPFPLYLISDEMGEAPLISVEVDEGEVLVAALFSSVERARDF
                     RERAAHLDLPGSLGTIEDREGLRRHALIARQAGAAYAVVDPASGLTEAIPVEELI"
     gene            1091..1645
                     /locus_tag="VHM69_03660"
     CDS             1091..1645
                     /locus_tag="VHM69_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739517.1"
                     /translation="MDSTIWDTGAWVCAAVLEVTGETLDVAEVTTWTHVLDAYGERIT
                     TTIFDRVFDPARIREREPYAGASKVLRALQVERGLEVHFVTHNDPEVMTPHLEPWLTT
                     HFGSGVGLTVTMEDKLRILENFDAFGLIDDRPDTLRRAADAGLWAATKIQPWNRDLVE
                     RRDDVHGFKEWWEVPELLPPLRGG"
     gene            1681..>2296
                     /locus_tag="VHM69_03665"
     CDS             1681..>2296
                     /locus_tag="VHM69_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739518.1"
                     /translation="MFRSPSVRRLMLLGPSLALAVLMLFHPSPYDDVAGELLPIAGWW
                     TLLHTVQFFLFAFVGASVWILVDGLRGAAAVVARVAAVVFILFYDIGDAVAGIATGIL
                     AGDAAGGALPEDAAVTAIDAIFSNLTKDVFFGIGISAWILAFVASAVALWRTGAPRLP
                     VVLLALPAFLLTFDHAFPFGALTFASFFFIALWLEMPRRDRIRPR"
BASE COUNT          338 a          734 c          753 g          471 t
ORIGIN      
        1 tggcttctcg agcacgagct atctggtcag cgtacagaac gcggtgccct ggcggcgccg
       61 cggcgtcgcg acttcttctg tggtcttctt ccgcacggta ggcggctcgc tcggggtcgc
      121 cgtgatgggc gccttgctga acgcttctct cggggagcgt taccgggccg ccgtggataa
      181 ggccgcaggg ggcgacgccg gcctcgagcg tctgctctcc gacccgaacg ccctactgca
      241 gcccgccgtg cggagccgca tccccgaggc tgtgtactcg gagctggccg gagccctcgc
      301 agcctcgctc tccccagcgt tctgggtgct gctcttcttc ggcctctcag ccctcgcagt
      361 ggcgacgctc ttcccgcgcg gaacggccaa ggacctggtg agtcgagagg aggattatgc
      421 gtgagacagg acgcgagttg accctcgacg acctccctcg tccgccgcta ttcaagctgg
      481 tcgaccccga gggaagggac gtcttccagc agaccgaagt cgggggagaa agcgccaggg
      541 tcggcgtcct cttctcggac agggagcttg cgggggagtt ctccgccggt gccagcgctc
      601 acggcatgca ggacctctcc ggcctcgatc cacgggaagt gtcagactgg ggttcggtgg
      661 aagacttcgc gctctcaggg gccgacttcg tgctggtcgt ctcggagatt ggcgcaggtc
      721 tcttctatgc cggggacctg gcgcacgtgg cacaggaggt gtcaggtgag atccctttcc
      781 cgctatattt gatctccgac gaaatggggg aggcgcccct gatctccgtc gaggtcgatg
      841 agggagaggt gctcgtggca gcgctgttca gttccgtgga gagagcccgc gacttccgag
      901 aaagggccgc ccaccttgac ctgcccggga gcctcgggac catagaggac agggaaggcc
      961 tcaggcgcca cgccctcata gcgcgacagg cgggggcagc ctacgctgtc gtggacccgg
     1021 cgtccggcct caccgaggcg atccccgtcg aggagctgat ctaaccctga aaccgacact
     1081 ctgctgtgat gtggactcta cgatctggga tacgggcgcc tgggtctgtg cggcggtcct
     1141 cgaggtcacg ggcgagacgc ttgatgtggc tgaggtgacg acctggaccc acgtcctgga
     1201 tgcttatgga gagaggatca ctaccacgat cttcgaccgc gtcttcgacc ctgcccgcat
     1261 ccgtgagcgg gaaccttatg cgggcgcttc gaaggtgctg cgtgcgttgc aggtagagcg
     1321 tggactagag gttcatttcg taacccacaa cgaccctgag gtcatgacgc ctcatctgga
     1381 gccctggctc acgacgcact tcggctccgg cgtgggtctc acggttacga tggaggacaa
     1441 gctccgtatt ttggagaact tcgacgcttt cggcctcata gacgacaggc cggataccct
     1501 ccggcgggcc gccgatgcag gcctgtgggc ggcgaccaag atacaaccct ggaaccgcga
     1561 cctcgtcgag aggagggacg acgtgcacgg tttcaaagag tggtgggagg tcccggagct
     1621 cctgccgcct ctccgaggtg gttaaatcca tgctcgatgt ccgcttctgg aggtgatgcc
     1681 gtgttccgtt ctccatccgt gcggcgcctg atgctgctcg gaccctccct cgctctggct
     1741 gtgctgatgc tcttccatcc gtcgccttac gacgacgtag ccggcgagct tttgcccatc
     1801 gccggatggt ggacccttct gcacacggtg cagttcttcc tgttcgcgtt cgtgggcgct
     1861 tcggtgtgga tactggtcga tggcttgcgc ggggccgccg ccgtagtcgc gcgggtggcc
     1921 gcggtggtct tcatcctgtt ctacgatatc ggggacgccg tggcgggtat tgccacaggc
     1981 attctcgccg gcgatgcagc gggcggcgct cttcccgaag acgccgccgt caccgcgata
     2041 gatgcgatct tctccaacct cactaaagac gtgttcttcg gtattggaat atcggcctgg
     2101 atcctggcct tcgtcgcgtc ggcggtcgcg ctgtggcgca ccggcgcgcc ccgtctgccc
     2161 gtagtcttgc tggccctacc ggcgttcttg ctcaccttcg atcacgcctt ccctttcggt
     2221 gccctcactt tcgcctcgtt cttcttcatc gccctctggc tggagatgcc gaggcgggac
     2281 cgcattcgcc cccgaa
//
LOCUS       DATAIR010000151         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_234990, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000151 DATAIR010000000
VERSION     DATAIR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234990"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VHM69_03670"
     CDS             complement(<1..753)
                     /locus_tag="VHM69_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2739519.1"
                     /translation="MIGATSRATEGFAPELFAVALYKGESPQDEAAAGVVSSGDFSGK
                     VGETALLFGQQGPAPRLLLVGLGDRDLVSLEKLRRAAATVARRARTLRLREAALSLPT
                     LPDTGTRDVARAAAEGAKLGLYRFDRHKTAVKDHALETFWLVAEDKNLEEAGEGAEVG
                     ESVAEGALFARDLANEPSNVATPEYLAERARGIAEKYAMELDVFDRAGIEEEGLTGLA
                     TVGRSASNEPRFIVLEHRKGGDDAPVVLVGKAV"
     gene            856..1629
                     /locus_tag="VHM69_03675"
     CDS             856..1629
                     /locus_tag="VHM69_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2739520.1"
                     /translation="MKLRPYLEFCVQTAYEAGRLTLGYFGTEASRPEFKADYTPVTAA
                     DREAEKLIRDRIWARYPAHAVVGEEYGTDDGADPDYRWIVDPIDGTKAFVRGVPLYGV
                     LIGLEIGGVCEVGAAYFPALDEMVCAASGEGCYRNGRRAHVSSTPTLAHGIAAFTDAA
                     SFEEHGRADAWGRLLSTAGTSRGWSDAYGHALVATGRVDLMLDPIMNPWDCGPFPPIL
                     REAGGYFGDWSGNETIYANEAMSTTTTLLPEVLRLLQGR"
     gene            complement(1674..>2556)
                     /locus_tag="VHM69_03680"
     CDS             complement(1674..>2556)
                     /locus_tag="VHM69_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX2739521.1"
                     /translation="GYFDLTTLDAATALRRETVQSLQQLGIKVEYSHHEVGISQHEID
                     LRFDDAMTMADKVMTYKTVVKEVATRHGVYATFMPKPLRDQNGSGMHTHQSLFSGGKN
                     AFFDPDNEYYLSSEAQAFIAGQLRHAREISIIFAQHVNSYKRLVPGYEAPVYIAWSRR
                     NRSALVRVPLYHPGQERATRMELRCPDPAANIYLCFAALLHAGLEGIEKGYELPEPME
                     RNLYHLTSEEREKMGIQSLPADLGEAIKEAENSELLHKALGEHTYTRLLQLKREEFED
                     YRVQVTPYELEKYLPVL"
BASE COUNT          427 a          840 c          819 g          470 t
ORIGIN      
        1 aacggccttc ccgacgagga cgacaggggc gtcatcgccc cctttgcggt gctccagcac
       61 gatgaaccgc ggctcattcg aggcggagcg cccgaccgtg gcgagccctg tgagcccttc
      121 ttcttcgatg ccggcccggt cgaaaacgtc tagctccata gcgtacttct cggcgattcc
      181 tcgcgccctc tcagccaggt actcgggggt cgccacgttg gacggctcgt tggcgaggtc
      241 gcgtgcgaac aaggccccct cagctacgct ctcaccaact tcggcaccct ctccggcctc
      301 ctccaggttc ttgtcttcag cgacgagcca gaaggtttcg agagcgtgat ctttcaccgc
      361 cgtcttgtgc ctgtcgaagc ggtaaagtcc gagcttcgcg ccctccgcgg cagcccgcgc
      421 gacatcccgt gtcccggtat ccggcagggt cggcagggag agggccgcct cgcgcagcct
      481 gagggtgcgg gcgcgtcttg cgaccgtcgc ggcggcgcgg cgcagcttct ccaggcttac
      541 gaggtccctg tcaccgagac cgacgagcaa gaggcgtggc gccggaccct gctgcccgaa
      601 gagcagggcc gtttcgccta ccttgccgct gaaatctccg ctcgagacca ccccggctgc
      661 ggcttcgtcc tgcgggctct cccccttgta cagggcgacc gcgaacaact cgggcgcgaa
      721 tccttccgtt gcgcggctag tggcgccgat catgaccaac tctggaccac ctctctagga
      781 cacacggccg cgtagattat aggggaaagg cagaatctat ctaatccgct gctcgtacaa
      841 tgctatctta ctggagtgaa gctgcgacct tacctggagt tctgcgtcca gaccgcgtac
      901 gaggccggca ggctcacgct cggctacttc gggacggagg cgtcgcgtcc tgagttcaag
      961 gccgactaca cgcccgtcac ggcggccgac cgcgaggccg aaaagctcat ccgagacagg
     1021 atctgggccc gctacccggc gcacgccgtg gtgggagaag agtatggcac cgacgacggg
     1081 gccgacccgg actaccgttg gatcgtcgat cccatagacg gcacgaaggc attcgtgcgg
     1141 ggcgttccgt tgtacggagt cctgatcggg ctcgaaatag gtggcgtgtg tgaggtgggc
     1201 gccgcgtatt tccccgccct cgacgagatg gtctgcgccg catcaggcga gggatgctac
     1261 cgcaatggcc gcagggcaca cgtttcgtcc acgccgactc tggcgcatgg catagccgcc
     1321 ttcaccgacg ccgccagctt cgaggagcac ggccgcgctg atgcgtgggg gcgtctgctc
     1381 tcgaccgccg gaaccagccg cggctggtca gacgcctacg gccacgccct cgtcgccacg
     1441 gggagggtgg atctcatgct cgaccccatc atgaacccct gggactgcgg cccattccca
     1501 cccatactgc gagaggccgg cggctacttc ggcgactggt cgggcaacga gaccatctac
     1561 gcgaacgaag ctatgagcac caccacgacg ctgctgccag aggtgctcag gctgttacag
     1621 ggccgatagg cacgtacaag aggcgcggct cacgccgcgc cccttgctga agcctacaag
     1681 accggcaagt acttctccag ctcgtacggt gtcacctgta ccctgtaatc ctcgaactcc
     1741 tcgcgcttga gctgcagcag gcgggtgtaa gtgtgctcgc cgagggcctt gtgcagtagc
     1801 tccgagttct ccgcctcctt gatcgcctct cctaggtcgg cggggaggct ctggatgccc
     1861 atcttctcgc gctcctcgga cgtcagatgg tagaggttgc gctccatcgg ctctggcaac
     1921 tcgtagccct tctcgatgcc ttcgaggccc gcgtgcagaa gcgcggcgaa gcagaggtag
     1981 atgttggccg cggggtcggg acagcgaagc tccatcctcg tggccctctc ctggccgggg
     2041 tggtacagag gaacgcggac cagagcggag cggttgcggc ggctccaggc gatgtagacg
     2101 ggggcctcgt agccggggac caggcgcttg taggagttaa cgtgctgggc gaagattatg
     2161 ctgatctcac gcgcgtgcct cagctggccc gcgatgaacg cctgcgcctc gctcgaaagg
     2221 tagtactcgt tgtcaggatc gaagaaagcg ttcttgccgc ccgagaaaag gctctgatgg
     2281 gtatgcatcc ccgagccgtt ctggtccctc aagggcttcg gcatgaacgt cgcatacacg
     2341 ccgtggcggg tcgcgacctc cttgacgacc gtcttgtagg tcatcacctt gtcagccatc
     2401 gtcatcgcgt cgtcgaagcg gaggtcgatc tcgtgctgtg agatgccgac ctcgtggtgc
     2461 gagtactcga ccttgatgcc gagctgctgc agcgactgga cggtctcacg cctcaatgcc
     2521 gtcgccgcgt cgagcgtcgt caggtcgaag tagccg
//
LOCUS       DATAIR010000152         7513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_378346, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000152 DATAIR010000000
VERSION     DATAIR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378346"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1225
                     /locus_tag="VHM69_03685"
     CDS             86..1225
                     /locus_tag="VHM69_03685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2739522.1"
                     /translation="MTHEDLRRGVREVCSRFPNEYWRDLDTRRRYPEEFVQAMTRAGY
                     LAALIPEEYGGMGLDLSAACAILEEVNLSGANAGPAHAQMYTMGTLLRHGSEKQKCTY
                     LSKIATGELRLQAFAVTEPEAGTDTTSIRTTATREGEDYVVNGRKVYISRVEQSDLMI
                     LLARTTPLDETERRSRGLSVFLVDLREAEGLTIKPRRVMMNNATSELVFEDLKVPMDS
                     IIGEEGEGFRYVLDGMNAERILIAAECIGDGRWFVQKSAERAGEREVFGRPIGKNQGI
                     QFPIARAYASVEAASLMVDKAASLFDRGEPCGAEANLAKLLAADASWEAANAALQTFG
                     GYGFDAEYDVERKFRETRLYQVAPISTNLILSHVAEHVLGLPRSF"
     gene            1228..2412
                     /locus_tag="VHM69_03690"
     CDS             1228..2412
                     /locus_tag="VHM69_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX2739523.1"
                     /translation="MLPLEGITVVSVEQAVAAPFATRQLADLGARVIKVERTGSGDFA
                     RSYDDVVKGLSSHFVWLNRSKESLALDLKKQAACQILDHLLERADVFVQNLAPGAAGR
                     LGFGTEVLRERQPGLIVCDVSGYGATGPYRDKKAYDLLVQCEAGLVSVTGTPETPSKA
                     GIAVADIAAGMYAYSSILAALFRRERTGEGAAIEVSLFEALAEWMGFPAYYAMYGDKE
                     PPRSGASHSSIAPYGPFECADGEVVFLGIQNQREWEKFCEGVLGRPEVAGDERFHNNS
                     RRVENREVLEREISTVFSGLSSEQAIDKLESAGIANARLRSVLDFLNHPQLKARDRWR
                     EVGSAAGPLHALLPPATMSGAEPVMAPVPEVGEHTANILSELGYDDTSLARLHREKVV
                     GG"
     gene            complement(2552..3082)
                     /locus_tag="VHM69_03695"
     CDS             complement(2552..3082)
                     /locus_tag="VHM69_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX2739524.1"
                     /translation="MKRTFGAVTVECVRGDITSQDDVDAVVNAANALLGPGGGVAGAI
                     HRIAGPGLAEEARPLGPIEPGEAVITGAHGLPNRHVIHTLGPVYGQDRPEAELLAGCY
                     RNSLALAEENGIESIAFPAISTGIFGYPVEAAARVALGAVRDEADSLDIVRLVRFVLH
                     SPSDLETHEKVLSEFE"
     gene            complement(3079..4236)
                     /gene="nagA"
                     /locus_tag="VHM69_03700"
     CDS             complement(3079..4236)
                     /gene="nagA"
                     /locus_tag="VHM69_03700"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX2739525.1"
                     /translation="MRLVALRGQIVTDYEVWPEGTLLLGEGSIADVSPDDSLVADAHE
                     VHDYGESLILPGFVDLQVNGAFGVDVASESSRLPQLSEALLSTGTTSYLPTVISSPEG
                     IYEESLPAIGAAVNGSSTGAEVLGVHLEGPFINLRKRGAHAAAYVVMPDKELLARLLD
                     LGPVRMITLAPELAGADKLMDLAANRGVVVSAGHSDVAFEPAYGALDGQVAGVTHLFN
                     AMSPMHHREPGLPGAAFAHPRAVCGLIADGLHVHPEMVGLAFRMLGPDRVCLVTDAIA
                     ATGMGDGEYRLATRTVYADGGVPRLGSGAIAGSVLTMKEAFKNILAFTGCTVPEAARM
                     TSTSPARLVGEGRRKGRLVPGYDADVTVLAPDLSVRAVWRCGKRAYSRDGG"
     gene            complement(4301..5638)
                     /locus_tag="VHM69_03705"
     CDS             complement(4301..5638)
                     /locus_tag="VHM69_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739526.1"
                     /translation="MSEARISGGKASRLAAFSWILYDFSNTIFSIAVLSFFFPLWVEE
                     QVGSGGFLSGPGLVNSATAISALFVVVTAPVLGAVADLRQRRVPYLILLTVIAVALTA
                     ALDVAGGVVVGLALFIAADLAYQSALVFYNALLPVVSAGRGAGRISGYGTAAGYIGTI
                     LALVVLTYFVENGEALRPLLGPLGGWLETGEEVYSNAFVPTAVLYLVFSLPAFFLVPD
                     PALRPRRPVGLGAAYRDVVRTVKNLRAYVGVGTFILATVLYTDAANTAIANMALYGRV
                     VFHMDGTQVRNLLLFSTVFAVVGSAGFGFLTDRIGPKRSLVGVLVLWLVAIVLSATAL
                     GAWMLFLAGPIVGIALGGMWTVGRVMLVALSPPEKIGEFFGLFSLAGKVSAVFGPALT
                     AVLLFVFGGGAFGYRISIGSLAVIMAAGLFFLLRVPDVSPESNVNEFSPESAS"
     gene            5809..6273
                     /locus_tag="VHM69_03710"
     CDS             5809..6273
                     /locus_tag="VHM69_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739527.1"
                     /translation="MKPWIKVALVTAAFAIPAMALGQVIWPPAPGGPSPTAGQLPFFL
                     FLAVFEALTFGLGVSFLLFGLAPLRRAVGGSTWRTWAIYITVGWILVSWWPHDNMHIH
                     NGDDLQGLLYIEYGFHVTLMLSGLILAYSLLTLLRPGDPGVKPGGATAARVR"
     gene            6304..6789
                     /locus_tag="VHM69_03715"
     CDS             6304..6789
                     /locus_tag="VHM69_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739528.1"
                     /translation="MIRPARGRASTPLHVVAALSLLAAMIHLWVIPEHFEVWWGYGTF
                     FLVSAIAQGLYAPGMLLWPNRVILLAGVAGNLVIVILWLVTRTSGIPLFGPHAGESED
                     VGTLDLVCTLAEVGIIAGLGALTLRDLPTERRIQIVVVLAVSALFFWHLLHLLAGSSA
                     H"
     gene            complement(6830..>7513)
                     /locus_tag="VHM69_03720"
     CDS             complement(6830..>7513)
                     /locus_tag="VHM69_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2739529.1"
                     /translation="TPQFAGTESVLLAAGILGATVMPHVIYLHSALTQHRVVGRTEEE
                     KKKIFRFELVDVVIAMAIAGTINASMLIMAAALFHSSGLTGVADIDKAFEQLRVLEGN
                     TASILFGVALLASGLSSSSVGTMSGQVVMQGFINRRIPLFLRRLVTMAPALVILAVGL
                     NPSRSLVISQVVLSFGIPFALIPLLIFCRNRGLMGALVNHRLTTAIATVVVTLIVSLN
                     VFLLYQTFS"
BASE COUNT         1417 a         2446 c         2399 g         1251 t
ORIGIN      
        1 gtaaagtcga tttcgtggtc atcgtctcgc agacaggccc ggaatgccac ccggcctcat
       61 cagcagcgcc ggggtaccac agcccttgac acacgaggat ctgcgcaggg gcgtcaggga
      121 ggtgtgctcc cgcttcccga atgagtactg gcgagatctc gacaccaggc gccgctaccc
      181 cgaagagttc gtgcaggcga tgaccagagc aggctacctc gccgctctca tacccgagga
      241 atacggcggg atggggctcg acctctcggc ggcctgcgcc atcctggagg aggtcaacct
      301 gagcggcgcc aacgcaggcc cggcccacgc ccagatgtac acgatgggta cactcctcag
      361 gcacggctcc gagaaacaga agtgtacgta cctgtcgaag atagccacgg gcgaactgag
      421 gctgcaggct ttcgccgtca ccgagcccga ggccgggacc gacacgacct ccatacggac
      481 gaccgcgacg cgcgaaggcg aggactatgt agtaaacggg cgcaaggtct acatctccag
      541 ggtcgagcag tccgacctca tgatcctgct cgcccgcacg acgcccctcg acgaaacaga
      601 gagacgctcc cgcggcctct ccgtcttcct cgtggacctg agggaggccg aaggtctcac
      661 catcaagccc aggcgcgtaa tgatgaacaa cgcgaccagc gagctcgtct tcgaagacct
      721 caaggttccc atggacagca tcatcgggga ggaaggagag ggtttccgct acgtactcga
      781 tggcatgaac gcggagcgca tcctcatcgc cgcagagtgc ataggcgatg gaaggtggtt
      841 cgtacagaag tccgcggagc gcgccggcga gcgcgaggtc ttcgggcggc ctatcgggaa
      901 aaaccagggc atccagttcc ccatagcgag ggcttacgcc agcgtcgagg ccgcttcgct
      961 tatggttgac aaggcggcct ccctcttcga caggggagag ccgtgcggcg cggaggcgaa
     1021 cctggcgaag ctcctggcgg ccgacgcttc atgggaggcc gccaacgcgg ccctccagac
     1081 cttcggcggc tacggcttcg acgcggagta cgacgtggag cgcaagttcc gcgagacgcg
     1141 tctctaccag gtggccccta tctccaccaa cctcatcctc tcccacgtcg ccgagcacgt
     1201 cctcggactc ccgaggtcgt tctgagtatg ttgccactcg aaggcatcac ggtcgtctcc
     1261 gtcgagcagg cggtcgccgc gcccttcgcc acgcgccagc tcgccgacct cggtgcgcgg
     1321 gtcatcaagg tggagcgcac ggggtcgggg gacttcgccc gctcctacga cgacgtcgtc
     1381 aagggtctct cgagtcactt cgtctggctc aaccgctcca aggagtcgct cgcgctcgac
     1441 ctcaagaagc aggccgcctg ccagatcctg gaccacctcc ttgaacgggc cgacgtcttc
     1501 gtccagaacc tcgcgccagg cgccgcggga cggctcggtt tcgggacgga agtcctgcga
     1561 gagaggcagc cagggctcat cgtgtgcgac gtctccggct acggcgccac tggcccgtac
     1621 agggacaaga aagcctacga cctgctcgtc cagtgtgagg ccggtctcgt ctccgtcacg
     1681 ggcacacccg agaccccgtc gaaggcaggc atcgccgtcg ccgacatagc ggcggggatg
     1741 tacgcctact cgagtatcct ggctgccctg ttcaggcgcg agcggactgg cgagggagca
     1801 gccatagagg tctcgctctt cgaggcgctc gccgagtgga tgggcttccc ggcatactac
     1861 gcgatgtacg gagacaaaga gccgccccgc agcggcgcca gccactcctc catagcccct
     1921 tacggtccct tcgagtgcgc cgacggcgag gtcgtcttcc tcggcatcca gaaccagagg
     1981 gagtgggaga agttctgcga aggggtgctc ggacggcccg aggtggccgg cgacgagcgc
     2041 ttccacaaca attcccgccg cgtcgagaac cgcgaggtcc tggagaggga gatctccacc
     2101 gttttcagcg ggctctcctc cgagcaagcc atagacaaac tcgaaagcgc cgggatcgcc
     2161 aacgccaggc tcaggtcggt gctggacttc ctgaatcacc cacagctaaa ggcccgcgac
     2221 cgctggcgcg aggtcggctc cgcggcaggt cccctccacg ccctcctccc gccggcaacg
     2281 atgtccggcg cagagcccgt gatggccccg gtccccgagg tcggcgagca caccgcaaac
     2341 atactctcgg agctcggcta cgacgacaca tccctcgccc ggctgcaccg cgaaaaggtc
     2401 gtgggcggct gaccccaaag tctgccgggt ttagatcctg cgccattggg tagtattaaa
     2461 tcttacgccc ggtggcccac agagtgagat atctttgtgt atcccccctc ctaagccacc
     2521 gggtcttctg ctatctcgaa gcgggtctcg cctattcgaa ctcggagagg actttctcgt
     2581 gggtttcgag gtcggacggg ctgtggagca cgaaccgcac caggcgtacg atgtccaggc
     2641 tatctgcctc atcccgtaca gcaccgagcg caacacgcgc cgccgcctcg acagggtagc
     2701 cgaagatccc ggtcgagatg gcaggaaagg cgatggattc tatcccgttc tcctcggcga
     2761 gggcgagaga gttgcggtaa cagcccgcca gtagctcagc ctcgggacgg tcctggccgt
     2821 agaccggtcc cagggtgtgg atgacgtggc ggttcggcag tccgtgggcg cctgttatta
     2881 cggcttcgcc aggctctatg gggccgagcg gtctggcttc ttccgcgagt ccgggtcctg
     2941 cgatcctgtg gatggcccct gcgacccctc cgcccggccc gagcagcgcg ttcgccgcgt
     3001 tgaccacagc atccacgtcg tcctgggaag ttatatctcc cctgacgcac tcgaccgtca
     3061 cggctccgaa cgtccttttc atccgccgtc cctggaatag gcgcgcttgc cgcaccgcca
     3121 gacggcccgc acggagaggt cgggcgcaag gaccgtcacg tcggcatcgt agccggggac
     3181 gaggcgaccc ttgcggcggc cctctccgac taacctcgca ggcgaagtcg aagtcatgcg
     3241 cgcggcctcg gggacggtgc agccggtgaa ggccaggatg ttcttgaatg cctccttcat
     3301 ggtgaggacg ctgcccgcga tggctccgct tccgagcctc gggacgccgc cgtcggcgta
     3361 aacggtcctg gtagccaggc ggtattcgcc atcacccatg ccggttgcgg caatggcgtc
     3421 tgtaacaagg cagacgcggt cagggccgag catacggaag gcgagcccga ccatctcggg
     3481 atggacgtgc agaccgtcgg cgatcaggcc gcataccgcc ctcgggtggg cgaaggctgc
     3541 ccctggcagg ccaggctcgc ggtggtgcat cgggctcatc gcgttgaaca ggtgcgtgac
     3601 gccggcgacc tgcccatcga gggcgccgta ggctggttcg aaggcgacgt cggagtggcc
     3661 tgccgagacc acaaccccgc ggttcgcggc caggtccatc agtttgtccg cgcctgcgag
     3721 ctcgggggcg agtgtgatca tacgcacggg accgaggtcg aggaggcggg ctagcaactc
     3781 cttgtcaggc attacgacgt aggccgcggc gtgcgcgccc cgcttccgca agtttatgaa
     3841 tggtccttcg agatgcacgc cgagcacctc ggcgcctgtg gaagacccgt tgaccgcggc
     3901 accgatcgct ggcagggact cctcgtagat gccttctggc gaggagatga ccgtcggcag
     3961 gtaggaggtg gtacccgtgg agagaagggc ttcggagagc tggggcaggc gcgagctctc
     4021 cgaggccacg tcaacaccga atgccccgtt cacctgcagg tccacgaagc cgggcaggat
     4081 cagggactcg ccgtaatcgt ggacctcatg cgcatcggcg acgagagagt cgtccgggct
     4141 cacgtccgcg atggaccctt ccccgagaag gagcgtaccc tcgggccaga cctcgtagtc
     4201 cgtgacgatc tgccccctga gcgcgactag cctcaactga gcgcccctgg catattgttc
     4261 cccctcatgc cgtacatctt ccgcccacgg cgcccgcgaa tcaagaggcc gactcagggg
     4321 agaactcgtt gacgttgctt tccgggctga cgtcggggac gcgtaggagg aagaagagcc
     4381 cggcggccat gataacggcc agcgagccta tggagatcct gtagccaaaa gcgccacccc
     4441 cgaagacgaa gagcaacacc gccgtgagcg ccggtccgaa gacggcggag accttccccg
     4501 caagcgagaa gagcccgaag aactccccga tcttctcggg cggcgagagg gcgaccagca
     4561 tcacccgccc caccgtccac atcccgccga gcgcgatccc cacaatggga cccgcgagga
     4621 agagcatcca cgctcccaga gccgtggctg agagcacgat agcgacgagc cagagaacca
     4681 gcacgccgac gagcgatctc tttggtccta ttctatccgt caggaacccg aaaccggccg
     4741 agcctacaac ggcgaagaca gtcgaaaaga ggagcaaatt cctcacctgc gtgccgtcca
     4801 tgtggaagac gacccgcccg tagagcgcca tgttggcgat cgccgtgttc gccgcgtcgg
     4861 tgtagaggac cgtcgccagg atgaacgtcc ccaccccaac gtatgcacgc aggttcttca
     4921 cggtacggac cacgtcgcgg tacgccgctc cgaggcccac gggacggcgc gggcgcagtg
     4981 ccgggtccgg cacgaggaag aaagcgggaa gggagaagac gaggtagagt acagccgtcg
     5041 gaacgaaggc gttggagtag acttcttcac ccgtctccag ccacccccca agcggtccga
     5101 gcagcggccg cagagcctcg ccgttctcga caaagtacgt gaggacgacg agggcgagga
     5161 tggtgccgat gtagccggct gcggttccgt agccactgat cctacctgcg ccccgccccg
     5221 ccgagacgac cggcagcagc gcgttgtaga agaccagcgc agactggtag gcgaggtccg
     5281 cggcgatgaa gagcgcgagt cccacgacga ccccgccggc cacgtcgagt gcggcggtga
     5341 gagcgaccgc gattacggta agcaggatca ggtaaggcac ccgccgctgg cgcaggtcgg
     5401 ctaccgcccc gagcaccggc gcggtaacga cgacgaacaa cgcggagatg gccgtggcgg
     5461 aattgacgag acccggcccc gagaggaatc ccccactgcc gacctgctcc tcgacccata
     5521 gcggaaagaa gaaggagagt acggctatcg agaatatagt gttcgagaaa tcgtagagga
     5581 tccaggaaaa cgccgcgagc ctcgaagcct ttccaccgga tatccgcgct tcgctcatct
     5641 ccgcggcagt atatagcagc cttctcgcca gatatcgttg gctgacttcg cgggcgttac
     5701 tcaatgattc cacgcgaccg aaagataccc catttggggg atgcgtagcg atatcttcgc
     5761 ccgctatttt gttagacgac gactcgaatt cgacagaagg agcgatcctt gaagccctgg
     5821 atcaaagttg ccctcgtcac agccgccttc gccatacccg ctatggccct cggtcaagtc
     5881 atctggcccc ccgcaccagg cggaccgtcg cccactgcgg gacagctccc gttcttcctc
     5941 ttcctcgccg tcttcgaggc gctgacgttc ggccttggcg tctcgttcct gcttttcggc
     6001 ctcgccccct tgaggcgggc cgtgggcggc tcgacctgga ggacgtgggc gatatacatc
     6061 acggtcggct ggatcctcgt ctcctggtgg ccgcacgaca acatgcacat ccacaacggc
     6121 gatgatctgc agggcctcct ctatatagag tacggcttcc acgtgacgtt gatgttgtcc
     6181 ggccttatcc tggcctattc actgctgacc ctcctcaggc cgggagatcc aggcgtcaag
     6241 ccaggcggcg ctaccgcggc gcgcgtcagg taatctcccc tggtgaaaag tggcgcaccg
     6301 gggatgatcc gcccagcgcg ggggcgtgcc agcacgcccc tgcacgtggt cgcagccctc
     6361 tctctgctgg cggcgatgat ccacctttgg gtgattcctg agcacttcga ggtgtggtgg
     6421 ggctacggca cgttcttttt ggtctcagcc atcgcccagg gactgtacgc ccccggcatg
     6481 ctgctctggc cgaaccgggt catcctgctc gccggcgtag ccggcaacct tgttatcgtg
     6541 atcctctggc tggttacacg cacgtcgggg ataccgcttt tcgggccgca cgccggtgag
     6601 agcgaggatg ttgggaccct ggatctcgtc tgcacgctcg ctgaggtcgg gatcatcgcc
     6661 ggcctcgggg cgctgacctt gagggacctt ccgactgaga ggcgcataca gatcgtggtc
     6721 gtcctggcgg tgtccgcgct gtttttctgg caccttctgc acctgctcgc cgggtcatcc
     6781 gcccactgat cttgacggag aacccggccg tttctaaccc acaagccggt tacgagaagg
     6841 tctggtagag aagaaatacg ttgagggaga cgatcagggt gaccaccacg gtagcgatag
     6901 cggtggtcaa acgatggttg accagcgctc ccatcagacc cctgttgcgg cagaagatga
     6961 gcaacggtat cagggcgaag ggtatgccga aagagagcac gacctggcta atgaccagcg
     7021 agcggctcgg gttgagcccg acggcgagga tgaccagggc gggcgccatc gtcaccagtc
     7081 gcctgagaaa gagcggtatc cgccggttta tgaaaccctg catcacgacc tgccccgaca
     7141 tggtgccaac ggatgaactg gagagccctg aggcgagaag ggcaaccccg aagaggatgg
     7201 aggcggtgtt gccttcgagg acacgcaact gctcgaaggc cttgtctatg tcggcgaccc
     7261 ctgtcaggcc actggagtgg aaaagagcgg cggccattat aagcatcgag gcgttgatgg
     7321 tacctgctat ggccatcgcg atgaccacgt cgacaagctc gaagcggaag atcttctttt
     7381 tctcctcctc cgtcctcccg accacccgat gctgcgtgag cgcggagtgg agatagatga
     7441 cgtgcggcat gacagtagct ccgaggatgc cggcggccag cagaacgctc tctgttcccg
     7501 cgaactgcgg cgt
//
LOCUS       DATAIR010000153         9061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_378350, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000153 DATAIR010000000
VERSION     DATAIR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378350"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..1273
                     /locus_tag="VHM69_03725"
     CDS             479..1273
                     /locus_tag="VHM69_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2739530.1"
                     /translation="MEALDRTGLINNPRIGVTAALREETGSIAQNLLGRFVRTDLDYV
                     KGVSEAGGVPVVLPPSLSLQAAEALLDSVDGLLLSGGPDLEPGYYGEGPIPELGTTIP
                     EWDALEMALLRLALQRGMPIFGICRGMQMLNVALGGTLYQDMPSQLGADVINHWQTTP
                     KNEVTHEVEVLDDSYLAKITDQQTVEVNSYHHQGIKGLAGVLTVAARSVDGVIEAIES
                     RDFSERWLVGVQWHPEGMRDIASGHRNLFEAYVCAAERHASHRTAA"
     gene            1626..2513
                     /locus_tag="VHM69_03730"
     CDS             1626..2513
                     /locus_tag="VHM69_03730"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX2739531.1"
                     /translation="MRHAVLGAGGVGGLVGGALAKAGEPVTLLVRPDRRGLYPERLSV
                     QSEMFGSFETPVRVADRLDDRFDVVWITVKATALDPALGTIPPEELGDGVVVPLLNGV
                     DHIERLRDRYGAERVLPGTIRVEAEKMGPGRVRHLSSFADVQVSPNPATRELAEALCE
                     ELRGAGLGCEVQDDEVTMLWSKLCFLAPFALATTASEGSLGVVRSDAGRWASLEECVY
                     EACAVGVAEGARVAPEPILTTLEGLPDGLRSSMQKDVAAGRVPELDAISGPILRGGSE
                     HGIDVPATRALVDRVVALE"
     gene            complement(2553..2903)
                     /locus_tag="VHM69_03735"
     CDS             complement(2553..2903)
                     /locus_tag="VHM69_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739532.1"
                     /translation="MQPLHLAPCMLEVEISPPPVGLQASMLVGVEQAVEVEACRPLIL
                     GLKDCLGVIQANLPNVPGKLAVGCGQFLRGGAQPTVRCVDLLDERVAGYECLLSSTPG
                     NSTIVPTNNDRRRN"
     gene            2988..3524
                     /locus_tag="VHM69_03740"
     CDS             2988..3524
                     /locus_tag="VHM69_03740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEX2739533.1"
                     /translation="MPEPEAHAYTDGASTGSRGPGGYGAVICWNGKSEEISGSEKHTT
                     NLRMELTAACVALETIDAGHTVTMYSDASYLVNCMRRGWYKKWRENGWLNHRKEPVAN
                     RDLWERLLEATQRHQEVRWRKVKGHSKTGGAHKTGNDRADELAVAAKKEAAGERGGGL
                     SRPGKREAPKRAGEATRQ"
     gene            3521..4399
                     /locus_tag="VHM69_03745"
     CDS             3521..4399
                     /locus_tag="VHM69_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX2739534.1"
                     /translation="MKETEPSCIFVCPERLYEGYVFDLDGTIYLGDELLPGARRLLEK
                     LRELRKKIIFLSNNPTRDPKMYAEKLTRLGLPTSEVEVVNTVLTMTRWLVQNHPEATV
                     FPISEEPLKRSLREAGIRMSERAEEIDVVIASFDRGFEYRKLQIAFDAIRFHKRARLV
                     TTNPDPYCPLPGGRGEPDAAAIVGAIEACTGARCEVNVGKPSPIMLKTVLDMLELESS
                     ECLMTGDRLYTDVRMALDAGMPSAVVLTGETTAEILAAETEENRPDWVVDRIDRLIPP
                     QMWEELGWTQDEGQSQ"
     gene            complement(4421..5767)
                     /locus_tag="VHM69_03750"
     CDS             complement(4421..5767)
                     /locus_tag="VHM69_03750"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX2739535.1"
                     /translation="MAGTMDLGRQHESSDERQTKIVDVLVDAFSKLMDADPDAFRQKF
                     RKMAAGPFAFYRGSACLFYADMEREEDRWADERTSRVWIQGDLHAENFGTYMDGNGVF
                     VFDVNDFDEAYLGHFTWDIKRMVASVALLAWMKAISDADIAYLIETYVRAYVEQVRYF
                     VDSDRDHEYALRLETTDGEIREILLETRLSTRVALLDRMTLIDENFERRFRRGPGIQE
                     LDDAERAKVQAAYEAYLETIPETKRFRSLTYEVKDIVGRRGFGIGSAGLPAYNVLVEG
                     PTQALENDVVLSLKQGNVAAPSRIVHDERIERYFKHHGHRTAVSQRALQAHADPWLGY
                     TEIDGVGFVVTELSPYVEDLDWSDLTEPEQMAPVLDYLGRATAKVHCVADKDSDPAIV
                     GFQTEDEIIEAVSGNEDEFVKEMVDFGTGYSEISRDDHRLFVDAFRNGQIPGLLDR"
     gene            6522..7007
                     /locus_tag="VHM69_03755"
     CDS             6522..7007
                     /locus_tag="VHM69_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739536.1"
                     /translation="MQPRIRPFEARDAEAVVDLSLRAWAPVFASLEQVLGSEIFRRLH
                     PDWREDQRRAVEDVLAAKKGRVWVAEVGASAVGFVAVEIYDPERRMGEISMLAVDPDY
                     QGGGIGTALTGFALERLKDAGMRVAMVETGGDPGHAAARRTYEKAGYVLLPIARYFKN
                     L"
     gene            7287..8123
                     /locus_tag="VHM69_03760"
     CDS             7287..8123
                     /locus_tag="VHM69_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739537.1"
                     /translation="MTTENTADPSAPDTIVLIHGLWVTSRSWEKWAERYEARGYRVLT
                     PAYPGFEVEVEVLNEDPSPIEALTIPGVVEHLESIVAALENPPIIMGHSAGGLFTQIL
                     LDHGYGAAGVVIDSAPAEGVRVTPVAQIRSFFPILSHPSTRHKAVGFTKEQFHNSFAN
                     TLSREDSDEAYERYHIPAPGSFVWAGPLANFTPGHQDTYVNFRNEERAPLLFIAGSED
                     NIMPPAVNQSNVKHYRHVKSVTDYKEFEGRSHFTVGEDGWEEVADYALEWAVEHAKLP
                     AG"
     gene            8326..>9061
                     /locus_tag="VHM69_03765"
     CDS             8326..>9061
                     /locus_tag="VHM69_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2739538.1"
                     /translation="MNIAIVGAMGLVGSQLVSLAKSRGHKVISLDIQTGTDVTAPEGL
                     EALAGAEAMVDVTQSPSLSEEDATAFFTRGAENLGKAATEARVQRSVVLSIIGVDKIV
                     AAETEPGTGFDGYYRAKFAQEQATLAFAPGPHVVRSSQFHDIARQAIGWGRDGDRSTI
                     RDLTIQPVAVSAMVEVLLAAATGAADAEVTEVAGPQREQLADLSTRYAVHVGDSVSIV
                     AAPVGDAVRHGILLPGSRARLVGPSFA"
BASE COUNT         1847 a         2642 c         2921 g         1651 t
ORIGIN      
        1 ggacgaggag aaagtaactg atacgggaag caagtgggct aaaggaggcg ttgagcgata
       61 aagttcctgg tgacctgctc ctcatctttc attgacttgt accgatgagc ttggcaccga
      121 cctgagatag ggaccacctc ccataagcag taaccgagac tcgaactgac gctgtgctac
      181 gcaagttggc ggaatataag acgaacgcta tgcatcggat ccagcgtcct tccttctgtg
      241 cctaatctcg cattcagctt tactcggtaa cctcgtgacg tacgtcatct ggcacatacc
      301 ctccggccct gaaaaagcct tcgaaaatac gtcgaatggg ggatgtggat gatccggggc
      361 gttgctagcc ttcttgcatc cggcaatatg ccaggatcgg agaagcgcgg agtatgaaaa
      421 gaaaggccag gactgatttc aaagtgaagc gctcgcgaaa agggtgagga tgggctcctt
      481 ggaagcacta gaccggacgg ggctgatcaa caacccaagg ataggggtga cggctgctct
      541 aagagaagaa acggggtcca tcgcgcagaa ccttctgggg cgttttgtgc gaacagactt
      601 ggattacgtc aagggtgttt ccgaagccgg aggggtgccc gtagtactgc caccttcact
      661 cagccttcaa gcggctgagg cgctcctcga tagtgttgac ggcctgctgt tgagtggtgg
      721 ccccgatctc gagccagggt actacggcga gggaccgata cccgaactcg ggaccactat
      781 ccccgagtgg gacgcgctgg agatggcgct gctcaggttg gctctccagc gggggatgcc
      841 tatcttcggc atttgccggg gaatgcagat gctcaacgtg gcgctaggcg gcactctcta
      901 tcaggatatg ccctcgcaac tcggcgctga tgtcatcaac cactggcaga cgacgcccaa
      961 aaacgaggtc actcacgaag tcgaggtcct ggacgactcc tacctggcga agatcacgga
     1021 tcagcaaacc gtcgaagtca actcgtacca tcatcaaggg atcaagggtc ttgccggcgt
     1081 ccttacggtg gccgctcgtt ctgttgatgg agtgatagag gcgatcgagt cacgcgactt
     1141 ctccgagcgc tggctggtgg gcgtccagtg gcatccagag gggatgcgcg acatcgcctc
     1201 cgggcaccga aacctcttcg aggcctatgt ctgtgccgcc gagcgccacg cctcacacag
     1261 aaccgcagct tagagaacgc tgttcgactt tcgtgctgga agccgacgcg taataagcag
     1321 gtcagccatc ggagtctgct cgaacggctt tactgcaacc gggctgatac gcaatggtac
     1381 gcaacggaca aagcgactcc gccagatcgt cgaaaacccg cctgaacaag cgcaattccc
     1441 agtcacagta ggacggccta gaacgagcgc aagcaaactc gtagctggag atgaggtcag
     1501 cggttcgagt ccgctcgtcg gctctcttcg ctgggcttag ataagccgaa ttctcggaac
     1561 gcatagcgtt cgctactcct ggaggctgta tacaatgggg tacaaacagt tgataggggt
     1621 gggagatgcg acacgcggtt ctcggagcgg gtggggttgg cggacttgtg ggcggtgcgc
     1681 tcgcgaaggc tggtgagccg gtgaccctgc tcgttcggcc cgaccgcagg ggcctctatc
     1741 ccgagcggct gagcgtacag agcgagatgt tcgggagctt cgagacgccg gtgcgggtcg
     1801 ccgacaggct cgatgatcgg tttgacgtcg tctggatcac agtcaaggcg acggcgctcg
     1861 atccggcact tggcaccatt cctccggagg aactgggaga tggggtcgtt gtgccgctgc
     1921 tgaacggcgt agatcatatc gaacggctgc gcgaccgcta cggtgccgag cgggtcctgc
     1981 cggggactat acgggtcgaa gccgagaaga tggggcctgg acgagtacga catctctctt
     2041 cctttgctga cgtgcaggtt tcgccgaatc cagcgacgcg agagttagcg gaggctttgt
     2101 gtgaggaact gcgtggcgcc gggttgggct gcgaagtgca ggacgacgag gtcaccatgc
     2161 tctggagcaa gctctgcttc cttgcgccgt ttgcactggc gaccacggcg tcggagggct
     2221 cgctcggggt cgtccggtcg gacgcgggtc ggtgggcaag cctggaggag tgcgtttacg
     2281 aggcctgcgc cgtgggagtc gccgaggggg ccagggtcgc tcccgagccg atcctcacaa
     2341 cactcgaagg tctgcctgac ggtttacgga gctcgatgca aaaagacgtg gcagctggga
     2401 gagtgccgga actcgatgct atctcgggac ctattctacg gggtggtagc gagcacggca
     2461 tcgacgtgcc agcgacccgt gccctcgtgg accgggttgt ggcgctagag taagggcgaa
     2521 caagctgtca ggcggtcctc tgccttctgc agtcagttcc gccgtctatc gttgttcgtt
     2581 ggtacgatcg tggagttgcc tggagtcgag gagaggagac actcataacc agcgacacgc
     2641 tcatcgagga gatcaacgca gcgtaccgtc ggctgagcgc cgccacggag gaactggcca
     2701 cagccgaccg cgagcttgcc gggcacgttc ggaaggttcg cctggataac gccgaggcaa
     2761 tccttgaggc caagaatgag aggacggcaa gcctctacct cgacggcctg ctcgacaccg
     2821 acgagcatcg acgcctggag gccgaccggg ggagggctga tctcgacctc cagcatgcaa
     2881 ggcgcgaggt ggagcggttg cacctgatcg ttcggctgct ggggacccaa gcgagcgagg
     2941 ggataccagg atgagtggag gggacatgat cgggagggtg tgcgtccttg cctgagccgg
     3001 aggcccacgc ctacaccgac ggggcatcca caggctcccg tggtcccgga ggctacgggg
     3061 ccgttatctg ctggaacgga aagtccgagg agataagcgg cagtgagaag cacaccacta
     3121 acttgaggat ggaactgacc gccgcttgcg tcgccctcga gacgatagac gcggggcata
     3181 cggtgaccat gtactcagac gcgtcctacc tcgtcaactg catgaggaga ggctggtaca
     3241 agaagtggcg ggagaacgga tggctcaacc accgcaaaga gccggtagcg aacagggacc
     3301 tatgggagag gctcctggaa gcgacgcagc gccaccagga agtgcggtgg aggaaggtca
     3361 aggggcactc caagaccggc ggggctcaca agaccgggaa cgaccgggcc gacgagctcg
     3421 ctgtggccgc gaagaaggag gccgcaggtg agagaggagg cggcctgagc cgacctggaa
     3481 agcgggaggc cccgaaacgt gcaggggaag ctacgcgaca atgaaggaga ctgagccatc
     3541 gtgcatattc gtatgtcccg agaggcttta cgaaggctac gtcttcgacc tcgacggcac
     3601 catctacctg ggcgatgagc tcttgcccgg ggcgaggcgc ctcctcgaaa agctgcgaga
     3661 gctccgtaaa aagatcatct tcctctccaa taaccctacg agggatccca agatgtacgc
     3721 cgagaagctc accaggctcg gtctccccac gtcagaagtc gaggtcgtca acacggtcct
     3781 cactatgacg cggtggctcg tacagaacca cccagaagcg accgtattcc cgatctcaga
     3841 ggagcccctc aagcgttcgc tccgggaggc tggtatcagg atgtcggaaa gagcggaaga
     3901 aatagacgtc gtgatcgcca gcttcgaccg tgggttcgag taccggaaac tccagatcgc
     3961 cttcgacgcc atccggttcc acaagagggc caggctcgtg accaccaacc cggaccctta
     4021 ttgcccgtta cctggaggtc gcggcgagcc ggacgcggcg gcaatagtcg gcgccatcga
     4081 ggcgtgcacc ggggccaggt gcgaggtgaa cgttggcaaa cccagcccca tcatgctcaa
     4141 aaccgtcctg gacatgctcg agctcgagtc gtccgagtgc ctcatgaccg gcgaccgact
     4201 ctacaccgac gtgcggatgg ctctggacgc cggaatgccc tcggctgtgg tcctgacagg
     4261 ggagacgacg gccgagatac tcgccgccga gaccgaggag aacaggccgg actgggtggt
     4321 cgatagaata gaccgcctca taccgcccca gatgtgggag gagcttggat ggactcaaga
     4381 cgaagggcag tctcagtaaa gatgggaccg cggcggcgtc tcatcggtcc aacaggcccg
     4441 ggatctggcc gttgcggaaa gcatcgacga acagcctgtg atcatcgcgg gagatctcgg
     4501 agtagccggt accgaagtcc accatctcct tcacgaattc gtcttcgttg ccgctgaccg
     4561 cctcgatgat ctcatcctcg gtctggaagc caacgatggc cgggtcggag tccttgtccg
     4621 cgacacagtg aaccttggcg gtcgcccgac cgaggtagtc cagcaccggc gccatctgct
     4681 ctggctcggt gagatctgac cagtccaggt cctcgacata gggcgaaagt tcggtgacga
     4741 cgaagccgac accgtctatc tcggtgtagc caagccaggg gtcggcgtga gcctgcaacg
     4801 cgcgctgcga gactgccgtg cggtgcccat ggtgtttgaa gtagcgttcg atgcgctcgt
     4861 cgtgcacgat ccggcttggc gccgcgacgt tgccttgctt caacgaaagc accacgtcgt
     4921 tctccagcgc ctgggttggg ccctccacca agacgttgta ggcggggagc ccggccgagc
     4981 cgatgccgaa gcccctgcgt ccgacgatgt ccttcacctc gtaagtcagg ctccggaagc
     5041 gcttggtttc ggggatcgtc tccaggtacg cctcgtacgc agcctgaact ttagcccgct
     5101 cggcgtcgtc gagttcctgg atcccggggc cgcgccggaa ccgccgctcg aagttctcgt
     5161 cgataagggt catcctgtcg agtagggcga cgcgagtact cagcctggtc tcgagcaata
     5221 tctcccggat ctcaccgtct gtggtttcca gccgcagcgc gtactcgtgg tcgcggtcgc
     5281 tgtcgacgaa gtagcgcacc tgctctacgt aggcccggac gtacgtctcg atcaggtacg
     5341 cgatgtcggc gtcagagatg gccttcatcc acgcaagcag ggccacgctg gcgaccatcc
     5401 gcttgatgtc ccaggtgaag tggcccaggt aggcctcgtc gaagtcgttg acgtcgaaga
     5461 cgaacacccc gttgccgtcc atgtaggtgc cgaagttctc cgcgtgcagg tcgccctgga
     5521 tccacactcg gctggtacgc tcatccgccc aacggtcctc ctcccgctcc atgtccgcgt
     5581 agaacaggca cgcgctgcca cgatagaatg cgaacgggcc agcagccatc ttccggaact
     5641 tctgacgaaa cgcatcggga tccgcgtcca ttagcttgga gaaagcgtca accagcacgt
     5701 cgacgatctt cgtctgacgc tcatcggagg actcgtgctg ccgtcccagg tccatagttc
     5761 cggccatggg gctcctttca ccgtcagtcg ccacggttca tgtctattag tccactaaac
     5821 agtagcacga cgtcaatcgg catgtgcccg tgtgattggc gtaggtggac cctcctcgcc
     5881 ggtatgggag agggagtcca ccagccgatc acttaaggag aagcgtccct gattgagacg
     5941 gaaacgaacc acatatgaac gacggtgtta cgataattcg agggtgctgg agacaccact
     6001 gtgacaccac cgtggacgtg atacggtgca acacagggca aggcgtagcg aagaaaaccg
     6061 gcctagatat gcgggatctg caacctgctg cacactcctg caacgcatga attatcactc
     6121 gtaatagcag gaaagcggtt cgagtccgct cgttggttct cttgctacgc cctagataag
     6181 tcgaatactc taatcgatac gggtctcgtt gctcgacagc ggaggccttc cgacaccact
     6241 gaggtagatc tggaaagtcg ttatccctcg gcagttcacg cgatggaccg tcgtgtctac
     6301 accgcggccg ccggcttgct gctgtcgttt tgatcgtggg gaaggagttc cccgagtctg
     6361 gtgatcgagg tcccagtgcg agctgagcta gaggtagcgc tgcgggctca agcactcact
     6421 cacgtgagtg cgtggaaagg ggatctcgga aggcaggttc gtaggtgccg cctgcacgct
     6481 atatgcaata cttcgttgcg ggtatcggga ggtaaaatcc tatgcagcca cggattcgac
     6541 catttgaagc gcgagacgcc gaggcggtag tcgacctctc gctgcgggcg tgggcgccgg
     6601 tcttcgcgtc acttgagcaa gtgctgggat ccgagatttt caggcgactt catccggact
     6661 ggcgagaaga tcagcggcgg gcggtggagg acgtcttagc cgcgaaaaag gggagagtgt
     6721 gggtcgccga ggtgggtgca agcgctgtcg gcttcgtggc ggtcgagatc tatgatcccg
     6781 agcgcaggat gggtgagatc tccatgctgg ccgtcgatcc cgactaccag ggcggtggca
     6841 tcgggaccgc cctaaccggg ttcgcgctcg agcggctcaa ggacgccggg atgagggtag
     6901 caatggtcga gacgggagga gatcccggac acgccgcggc gcggcgcacc tacgagaaag
     6961 ccggctacgt cctcttgccg atagccaggt acttcaagaa cctgtaggta cgaggggtca
     7021 tgatgtcccc ggcggcgatt accgatatgg ggaggcccgg ccttagccac aggaggccat
     7081 tattcacccg agtggagaag ctgtccaaaa actcctcata gacaccaacc gaaggcctta
     7141 cggagttcta cgaagttcgc gcgacgttcg cttgagtgct tcttcactac ttcgctcggc
     7201 gtagcttctc actacgctct cactacgctc ccctagtagg cttcgggtta ccccgaagtg
     7261 gtgcggtcca agaaaaggag gtcatcatga ctaccgagaa caccgccgac ccgagcgccc
     7321 cggacaccat cgtcctgatt cacgggctgt gggtgacctc acgaagctgg gagaagtggg
     7381 cagagcgcta cgaggctcgc ggctaccgcg tgctaacccc ggcctaccca ggcttcgagg
     7441 tagaggtgga ggtgctcaac gaggatcctt cgcctatcga ggcgttgacc atccctgggg
     7501 tcgtcgagca cctcgagagt atcgtagccg cgctagagaa cccgccgatc atcatgggcc
     7561 actcggcagg cggcctgttt acccagatcc tgctcgacca cggttacggc gcagcaggcg
     7621 tggtcataga ctccgcgcca gccgagggcg tcagggtaac cccggtcgca cagatccgct
     7681 cgttcttccc aatcctgagt cacccctcca cccgccacaa ggctgttggg ttcaccaaag
     7741 agcagttcca caactccttc gccaacactc tgagccggga ggactcagac gaggcctacg
     7801 agcgctacca catccccgct ccgggcagct tcgtgtgggc tgggcccctc gcgaacttca
     7861 cgcctggcca ccaggacacc tacgtgaact tcaggaacga agagcgggca cccttgctct
     7921 tcatcgcagg gagcgaggac aacatcatgc cgcctgcggt caaccagtct aacgtcaagc
     7981 actaccgtca cgtcaagagc gttacggact acaaagagtt cgaaggccgc tcgcacttca
     8041 cggtagggga ggatggctgg gaggaggtcg ctgactacgc cctcgagtgg gccgtcgagc
     8101 acgcgaagct cccggcgggt tgagcgaagc gcccgtcacg cacacggatc gtctcaacga
     8161 agatctgctg gcgttcatta aggagtgaga ttcatcagcc cgggcggaga ggtccgaagc
     8221 aggcctctct gcctcaagga ggcactctgg gagaggatct agtattgcga agaatggaag
     8281 catcggcatc ggtggcaatt gcaacccttc ggcaaggagg aagtcatgaa catcgctatt
     8341 gttggggcca tgggcctggt cggttcgcag ctggtcagcc tggcgaagag tcgtggccac
     8401 aaggtgatct ccctcgatat acagaccggc accgacgtga ccgcgcccga aggcctggag
     8461 gctctggcag gggcagaggc tatggtcgac gtcacccaga gcccaagcct gagtgaggag
     8521 gatgccacgg cgttctttac gcgcggtgcc gagaacctcg gtaaggccgc gaccgaggct
     8581 cgggttcagc gctcagtggt gctctcgatc atcggcgtcg ataaaatcgt ggccgcggag
     8641 acagagccgg ggaccgggtt cgacggctac taccgggcga agttcgctca ggagcaggcg
     8701 actcttgcgt tcgcgcccgg accgcacgtt gtccgctcgt cacagtttca cgacatcgcg
     8761 cggcaggcga tcggatgggg ccgcgatggg gatcgatcta ctatcagaga tctcacgatc
     8821 caacccgtgg cggtcagcgc aatggtggag gtgctattgg ctgccgccac gggcgccgcg
     8881 gacgccgagg tcacggaggt cgccgggccg cagcgcgaac agcttgcgga cctctcgacc
     8941 aggtatgcgg tccacgtcgg ggactccgtc tcaatcgtcg ctgcgccggt gggcgatgcg
     9001 gtgcgacacg gcatcctact gcccggatct cgggctcgcc tggtcggtcc gtcctttgcc
     9061 g
//
LOCUS       DATAIR010000154         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_101722, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000154 DATAIR010000000
VERSION     DATAIR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101722"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VHM69_03770"
     CDS             complement(<1..653)
                     /locus_tag="VHM69_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739539.1"
                     /translation="MPEENEGAVAKILEARGISKLFGGLVAVSSVDFDIPERSIVSLI
                     GPNGAGKTTFFNMVSGLYEPSAGTFTFEGRDITRLGAHKRAQLGIGRTFQNIRLFDTM
                     SAQDNVLAGMHTRLHSTILGSIFRTPGHRREEREARKRAGDYLDFVGISGRETFAKNL
                     SYGDQRRLEIARALASEPSLLLLDEPTAGMNPQETARLTDLIRRLREERGISILLIE"
     gene            complement(646..1806)
                     /locus_tag="VHM69_03775"
     CDS             complement(646..1806)
                     /locus_tag="VHM69_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2739540.1"
                     /translation="MTLLRRYWLPSLLIAIAFVFPIVDEAFDLLLIYPVIIITVYVML
                     ALGLNIVVGFAGLLDLGFVAFYALGAYVVGWFASSQFKQVSFSFGTAASSLTGEPLQG
                     IHLSFWFLLILAGSFTALCGVIIGAPTLRLRGDYLAIVTLGFGEIIPRFFQNGDDLGG
                     FNLTNGTIGIKAVDPPGFPFLPESLDAWKQFGTLDLHPWYYTILVMVLIAIYVNIRLR
                     DSRLGRAWISVREDETAAAAMGINPVTTKLWAYALGAVFGGLAGAFYGSFIASIFPTS
                     FSFNISILVLCMVVLGGMGNIYGVILGAIALQGLNFYLLPKVNEWVHAIGDAVGSPLL
                     SQADIPKYNFFLCGIILVLMMLLRPEGIIPNRQRAEELHGEDAGAMQGVSRA"
     gene            complement(1808..2893)
                     /locus_tag="VHM69_03780"
     CDS             complement(1808..2893)
                     /locus_tag="VHM69_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2739541.1"
                     /translation="MTTDEATKTSVGTLIESAKSSSWQSRIAVALVVALLVLLLREAA
                     QEPSQFASQLIIGITNGAIIALIALGYTLVYGIIELINFAHGDNFMIGSFVGLTVLSG
                     TFLGLEFFAPIGAGAGTFLKVAGVLLALLVAMIVCGLLNVGIERVAYKPLRNSPRLAV
                     LITAIGMSFILQNLGLVWKGPAQVPFPELISDANILSGDSIIFRWKDLFIVGVTVPLL
                     VGLSYFISNTKQGKAMRAVAQDKEAASMVGINVNRTISITFLLGGILAGASGVMYGLF
                     NGITVFNVGFTQGLYAFTAAVLGGIGNLSGAVVGGFLIGIIGSMADQYIGVEWTAIVI
                     FSVLILVLVFRPRGLLGDRSAVQGGGE"
     gene            complement(2951..>4023)
                     /locus_tag="VHM69_03785"
     CDS             complement(2951..>4023)
                     /locus_tag="VHM69_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2739542.1"
                     /translation="NAIELAIQQRDGKAGDIKIDYESFDDATAQAGQWDEAKCGENAQ
                     KAAQDEEIVGWIGPFNSGCAAVEIPILNRAGLGMISPANTYIGLTKPGGASDEPEKYY
                     PSGERNYARVIVADDKQGKAGVALMQEEGFESVYILDDKETYGKGLADQFEKAAEDQG
                     VEVLGREGIDGSAPNYRSLMNKINQADPDAIYFGGIIENNAAQLIKDKVGAGMSNEDV
                     AFIGPDGIFVDELLTQAGGAAEGIYVTFGGLPESELSAEGQEFVQTYESKYDDQIQPY
                     TAYAYEAANVMLDAIARAGKDGEVDRDEVVQEIFATEDYEGVLGTWSFDDEGDTSLTE
                     LSVQKVENGKFVLDNVLDVADL"
BASE COUNT          775 a         1263 c         1226 g          759 t
ORIGIN      
        1 tgctcgatga gcagaatgga gatcccacgt tcttctctga ggcgccggat cagatccgtc
       61 agcctggccg tctcctgcgg gttcatgccg gccgtcggct cgtcgagcag gagcaagctc
      121 ggctcagagg cgagcgcccg cgctatctcc aggcgccgct ggtcgccgta ggagaggttc
      181 ttggcgaacg tctcccgccc gctgataccg acgaagtcga ggtaatctcc ggccctcttt
      241 ctggcttccc tctcctctcg acgatgcccc ggcgtacgga agatcgagcc gagaatggtg
      301 ctgtgaagac gggtgtgcat cccagcgagc acgttgtcct gggcgctcat cgtgtcgaag
      361 agcctgatgt tctggaacgt gcgcccgatt cccagctgcg ccctcttgtg ggcgccgagc
      421 ctggtgatgt ccctgccctc gaacgtgaag gtgcccgcgc tgggctcgta gagtccgctg
      481 accatgttga agaacgtcgt cttgcccgcg ccgttgggcc cgatcaggct gacgatagag
      541 cgttcgggga tatcgaagtc gactgagctg actgccacga gcccgccgaa gagcttgctt
      601 attcccctgg cttcgaggat cttcgcaaca gcgccttcgt tctcctcagg cacgggacac
      661 tccttgcatc gcgccggcat cttcaccgtg caactcctcc gcgcgctggc ggttgggaat
      721 tatgccttcc ggtctcaaga gcatcatcag gaccaggatt atgccgcaca ggaagaagtt
      781 gtacttcggg atatccgcct ggctgagaag tggactcccg acggcgtcgc cgatggcgtg
      841 gacccactcg ttgactttcg gcagcaggta gaagttcagg ccctgcaggg ctatagctcc
      901 aaggatgaca ccgtatatgt tgcccatacc gccgaggacg accatgcaaa gcaccaggat
      961 cgagatgttg aaagagaagc tcgtcgggaa gatgctcgcg ataaacgagc cgtagaaggc
     1021 gccggccagt cctccgaaga ccgcaccgag ggcgtacgcc cagagcttcg tcgtcaccgg
     1081 gtttatgccc atcgcagcgg cggcggtctc gtcctcgcgc accgagatcc aggcccggcc
     1141 gagccgcgag tccctaagac ggatgttgac gtagatggcg atcaggacca tcaccagtat
     1201 ggtgtagtac cagggatgaa ggtccagggt cccgaactgc ttccaggcgt ccagggactc
     1261 ggggaggaaa gggaagcccg gcgggtcgac ggccttgata ccgatggtcc cgttcgtcag
     1321 gttgaaaccg cccaggtcgt cgccgttctg aaagaagcgg gggatgatct ccccgaaccc
     1381 gagcgtgact atggccaggt agtctcctct gaggcgcagc gtgggcgcgc cgatgatcac
     1441 gccgcacagc gcggtgaaag atccggcgag gatcaggagg aaccagaatg agaggtgtat
     1501 cccctgcaac ggctctcccg tcagcgagct cgccgcagtc ccgaacgaga agctcacctg
     1561 tttgaattgg ctggaggcga accagccaac gacgtaggcg ccgagggcgt agaaggcgac
     1621 gaagccgagg tcgaggaggc ccgcgaaccc aaccacgatg ttcaacccga gcgcaagcat
     1681 cacatacacc gtgataatga tgacggggta gatcaggagc aggtcaaaag cctcgtccac
     1741 gatcgggaag acgaacgcga tcgcgatcag caacgaaggt agccagtacc tcctgaggag
     1801 ggtcaagcta ctcgcctccc ccctgcacgg ccgagcggtc gccgagtagc ccgcgcggcc
     1861 tgaacacaag gaccagtatc aagacgctga agatcacgat cgccgtccac tcgacgccga
     1921 tgtactggtc tgccatggag ccgatgatcc cgatgaggaa cccgccgacc acggcaccgc
     1981 tcaggttgcc tataccgcca agaacagccg ccgtgaaggc gtacagtccc tgtgtaaagc
     2041 cgacgttgaa gaccgtgatg ccgttgaata gcccgtacat cacgccggac gccccggcca
     2101 ggatccctcc gaggaggaag gtgatggaga tcgtgcggtt cacgttgatg ccgaccatcg
     2161 aggcggcctc cttgtcctgc gcgactgccc gcatcgcttt gccctgcttg gtgttactga
     2221 tgaaatagga gaggccgacg aggagtggca ccgtcacccc gacgatgaac aggtccttcc
     2281 agcggaagat tatcgagtcc cccgacagga tgttcgcgtc ggagattagc tcggggaaag
     2341 gcacctgggc cggtcccttc cacacgagcc cgaggttctg caagataaaa gacatgccta
     2401 tagcggtgat caggacagcc agccgcggcg aattgcgcaa gggcttgtac gccacacgct
     2461 ctatcccaac gttcaacagg ccgcatacga tcatggcgac gagtagggcg agcagcacgc
     2521 cggcaacctt caggaacgtt ccggcgccgg cccctatcgg cgcgaagaac tcgaggccga
     2581 gaaacgtgcc tgagagcacc gtcagcccta cgaaagagcc gatcatgaag ttgtcgccgt
     2641 gggcgaagtt gatcagctcg atgatcccgt aaaccagcgt gtagccgagt gctatgagcg
     2701 cgatgatcgc cccgttggtg atcccgatga tgagctggga ggcgaactgg ctgggctcct
     2761 gagcggcctc cctcagcagc agaaccagta acgcgaccac gagggccacg gcgatgcggg
     2821 actgccagct cgaagatttc gcgctctcga tgagggtccc gacgctggtc ttcgttgctt
     2881 catccgtcgt catcccggat cctccataga ggtccggggg ccggtaagac cggcccccgg
     2941 ctctccccct ctagagatcg gcaacgtcga ggacgttgtc gagcacgaac ttgccgttct
     3001 cgaccttctg gaccgagagc tccgtcaggg acgtatcgcc ctcgtcgtcg aagctccagg
     3061 tcccgagaac cccctcgtag tcctctgtgg cgaagatctc ctgcaccacc tcatcgcggt
     3121 ctacctcgcc gtctttgccc gctcgtgcta tggcgtcgag catgacgttg gccgcctcgt
     3181 aggcgtaagc ggtgtagggc tgtatctggt cgtcgtactt ggactcgtac gtctggacga
     3241 actcctggcc ctccgccgag agttcgctct cgggcaagcc gccgaacgtg acgtagatgc
     3301 cctcggccgc gccgcctgcc tgagtgagga gctcgtcgac gaagatgccg tcaggcccga
     3361 tgaaggcgac gtcctcgttg ctcatcccgg cgccgacctt gtcctttatg agctgcgcgg
     3421 cgttattctc gatgatgcct ccgaagtaga ttgcatcggg gtctgcctga ttgatcttgt
     3481 tcatcagcga gcgatagttg ggcgcagatc cgtcgatacc ctcgcgtccg agaacctcta
     3541 caccctggtc ctctgcggcc ttctcaaact ggtcggcgag acccttgccg taggtctcct
     3601 tgtcgtcgag gatgtagacg gactcgaagc cctcctcctg cataagggcg acgccagcct
     3661 taccctgctt atcgtctgcc acgatcacac gcgcatagtt gcgctcgccc gaaggatagt
     3721 acttctcggg ctcgtcagac gcgccaccgg gcttggtcag gccgatatac gtgttcgcgg
     3781 ggctgatcat gccgagaccc gctcgattga ggatcggtat ctcaactgct gcgcagcccg
     3841 agttgaaagg cccgatccaa cccactatct cctcgtcctg ggcggccttc tgcgcgtttt
     3901 ccccgcactt ggcctcatcc cactgcccgg cctgcgccgt ggcgtcgtcg aaggactcat
     3961 agtcgatctt tatgtccccg gccttgccat cccgctgctg tatggcaagc tctatggcgt
     4021 tga
//
LOCUS       DATAIR010000155         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_101734, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000155 DATAIR010000000
VERSION     DATAIR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101734"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHM69_03790"
     CDS             <1..244
                     /locus_tag="VHM69_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739543.1"
                     /translation="PSFVLGIVPAQNQVVIHTRDGTAGAVSSVLDRVKGLKILGTIAG
                     QDTVLAITPDNHAAGEVANLIADVCRAKTRPAGNVE"
     gene            245..1159
                     /locus_tag="VHM69_03795"
     CDS             245..1159
                     /locus_tag="VHM69_03795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739544.1"
                     /translation="MKAGERAVYRVRPAGMEDVEFLWDMLYEAACWRPEGPRPPKEEV
                     FTTPELAHYVDGWGRPGDAAVIAFDPDDGAKVGAAWYRLMPPGDPGYGFVEAATPEIS
                     IAVVPDRRGTGAGEVLLGALMERARSEGFGSLSLSVEEDNQAIKLYRRAGFKELHLSD
                     GALTMSADLKDAAATKPTLSLTVLDGRLAVCRLDVGAEIPSWATGASFFSVTRTEDEL
                     SIVCPEENVPENISQEKGWRALKLEGPFELSTVGILASVAEPLAAAGASIFAVSTFDT
                     DYVLVRETQQDLAISTLREHGHEVREGG"
     gene            1156..1935
                     /locus_tag="VHM69_03800"
     CDS             1156..1935
                     /locus_tag="VHM69_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2739545.1"
                     /translation="MSAKVRITFLRHATFVFEAGGLRVMVDPMLAPAGAAEPVANTPN
                     QRRNPLVDLPFDEEETLALLEEIDAVLVTHTHNDHWDGRARDLIPKQTQILCQPEDEN
                     KIRAAGFQQVTPVEDDLEWRGLRISRTGGRHGTGEIGRHMAPVSGFVVGNEDSPGLCI
                     AGDTIWCPEVEEALGVHGPDVIVVNAGAARFVEGDPITMTAEDVVRVCRALPDALVVA
                     VHMEAINHCLLTRAGLEAALREESLVSRIKVPADGETFEVP"
BASE COUNT          393 a          582 c          658 g          343 t
ORIGIN      
        1 ccctagcttc gtcctcggca tcgtgccggc gcagaaccag gtcgtcatcc atacccgcga
       61 cgggactgcc ggcgccgtct ccagcgtcct cgaccgggtg aaggggctca agatcctggg
      121 caccatcgca ggccaggaca cagtgctggc gatcacgccc gacaaccatg ccgcaggcga
      181 ggtggctaac ctcatcgccg acgtctgccg ggcgaagacg cggccagccg gtaacgtcga
      241 gtagttgaag gcgggtgaga gagccgtata cagggtccgt cctgccggga tggaggacgt
      301 agagttcctg tgggatatgc tctacgaggc cgcatgctgg cggcccgaag gcccgaggcc
      361 tccgaaagaa gaagtcttca ctacacccga actcgcccac tacgttgatg gctggggcag
      421 gccgggagac gcggcggtta tagcgtttga tcctgatgat ggtgcgaagg tcggggccgc
      481 gtggtacagg ctgatgccgc ctggagatcc cggctacggt ttcgtagagg ccgcaactcc
      541 ggagatctcc atcgccgtag tccctgaccg gagggggacc ggagcgggtg aagtcttgct
      601 cggagccctg atggagaggg ctcgttccga gggtttcggg tcgttgagcc tgagcgtcga
      661 ggaggacaac caggcgatca aactctacag gcgcgcaggc ttcaaggagc tgcatctctc
      721 cgatggcgca ttgaccatga gtgcggacct caaagacgcc gcggcgacga aaccgacact
      781 ctccctgacg gtgctcgatg ggcgtcttgc cgtctgccgt ctggatgtcg gggcggagat
      841 cccgtcttgg gcaaccggag cctccttctt ctccgtaacg cgcactgagg atgagctctc
      901 catcgtctgc cctgaggaaa acgtacctga gaacatctct caagagaaag gctggcgcgc
      961 cttgaagctg gagggcccgt tcgagctctc gacagtcggt atcctggctt cggtggcgga
     1021 gccgctcgca gcggcaggcg cgagcatctt cgccgtctcc accttcgaca cagactacgt
     1081 gctcgtcaga gaaacgcaac aggatctcgc cattagtact ctgcgcgaac atggacacga
     1141 agtacgagag ggagggtgag cgcaaaagtg cggatcacgt tcctcaggca cgcgacgttc
     1201 gtcttcgagg cgggcggtct tcgggtgatg gtggacccca tgctggctcc tgccggggcc
     1261 gcggaacccg ttgccaacac gccaaaccag cggcgcaacc ctctggtgga ccttccgttc
     1321 gacgaagagg agaccttagc gctgcttgaa gagatcgacg cggtgctcgt cacacatacc
     1381 cacaacgacc attgggacgg gagggcgcgg gaccttatcc ccaaacagac ccagatcctg
     1441 tgccagccgg aagacgagaa caaaattagg gccgccggct tccagcaggt cacaccggtc
     1501 gaggacgatc tggaatggag aggtctgcgt atctcacgga cgggggggcg gcacggcaca
     1561 ggtgagatcg gacgccacat ggctcctgtt tcaggtttcg ttgtggggaa tgaagactcg
     1621 cccggtctct gcatagcggg agatacgatc tggtgccccg aggtcgagga agcacttggt
     1681 gtccatggcc ccgatgtaat cgtcgtgaac gccggcgcgg cgcggttcgt ggaaggcgat
     1741 cccatcacaa tgaccgcgga agacgtggtc agggtgtgcc gcgccctgcc tgacgcgctg
     1801 gtcgtcgcgg tgcacatgga ggcgataaac cactgcctgc tcacacgcgc cggcctcgaa
     1861 gctgcgctgc gcgaggagtc tctagtctca cggataaagg tccccgctga cggcgaaacg
     1921 ttcgaagtgc catagatacg tgtcaggagg aatcatgctg gaaggaaaga aggtcg
//
LOCUS       DATAIR010000156         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_9483, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000156 DATAIR010000000
VERSION     DATAIR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9483"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VHM69_03805"
     CDS             <1..724
                     /locus_tag="VHM69_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739546.1"
                     /translation="GTGKTNLTLGSGGGLQPAWSPDGNRIVYARVDVTSGVNNQNIYV
                     MDADDGGNKTPVDTTPRKDAEHDWQPMPDCTNTNATTGNDVITGTAGKDVLCGGDGND
                     TINGAGGNDIIMGEAGNDKLVGGSENDILNGGSGADTALYTGTIPVTANLTTLFATGV
                     GSDTLPGIERLTGSSAGDELRGSLAANVLVGGDGADEILGLGGNDKLHSRDGRKNDTV
                     NGGPGTDRCTTDNREISIKSCE"
     gene            781..1422
                     /locus_tag="VHM69_03810"
     CDS             781..1422
                     /locus_tag="VHM69_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739547.1"
                     /translation="MASRVRSGAIRLGRKTIFALGLAVILAVVLGVATTALAAVPGDP
                     FRLGRINSIDRLSTLAGTTANAMLRVDNDGTGPALSLGAGANRPPIVVNAAAGTATNL
                     DADQLDGQDSTDFLPAEIYEKSASQTISPDSSAGTTATCDTGDVAVSGSYAILGSRLD
                     VTSERRVDDRFDEETGQTNPSGWHVIAENPDTSAEGTITVYVNCADRAPVHSP"
     gene            1525..>2135
                     /locus_tag="VHM69_03815"
     CDS             1525..>2135
                     /locus_tag="VHM69_03815"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEX2739548.1"
                     /translation="MVAFLILLLFAVIFLVAGGVMLYFRNRTKQKSALMSQTETSASA
                     DVSGLAPGTLVEVKGTLRCEEPLTSEMAEKSCAYYSSKVIREYMERDYDDDDHGGSDR
                     RSEVIAQNEQFAPFLVEDTSGTVAVNAEGAEVDARQVVDRFERDTGNEGSIALGGMTI
                     NLGGGERTIGYRYTESILPIDSSVYVLGTVQDGGGIGAPSSGEG"
BASE COUNT          460 a          651 c          701 g          323 t
ORIGIN      
        1 cggaaccggc aagacaaacc tgaccctagg cagcggcggc ggcttgcagc ccgcctggtc
       61 gcccgacggg aacagaatcg tctacgcgcg cgtcgacgtg accagcggcg tgaacaacca
      121 gaacatatac gtcatggacg ctgacgacgg cggtaacaag acgccggtcg acactacccc
      181 acgtaaagac gcagagcatg actggcaacc gatgccggac tgcaccaata ccaacgccac
      241 cacaggcaac gacgtcatta ccggaacggc aggaaaggac gtgctctgcg gcggagacgg
      301 caacgacacc ataaacggcg ccggcggcaa cgacatcatc atgggcgagg ccggcaacga
      361 caagctcgtc ggcggatcgg agaacgacat cctcaacggc ggcagcggcg cggacaccgc
      421 actctacacg ggcaccattc ccgtcacagc caacctgacg acgctgttcg ctacgggcgt
      481 ggggtcggac actctgcccg ggattgaaag gctcaccggc tcgagtgctg gtgatgagct
      541 cagaggctct ctcgccgcga acgtgctcgt cggcggcgac ggtgctgacg agatcctcgg
      601 tctcgggggc aacgacaagc tccactcccg cgacgggcgc aagaacgaca ccgtgaacgg
      661 cggacccggt acggacaggt gcaccaccga caaccgggag atctcgatca agagctgcga
      721 gtaggcgggg cgaagcaacc ggccgggatc gccccccggc cgggaaagga gagacagcgg
      781 atggcgagca gggtgagatc cggagcgata aggttgggca ggaagacgat cttcgccttg
      841 ggccttgcgg tcattctggc cgtggtgctc ggcgtcgcga ctactgccct ggccgcggtg
      901 ccaggcgatc cgttcaggct cggtaggatc aactccatag accggctctc gaccctggcc
      961 gggacgacgg ctaacgcgat gctacgcgtt gacaacgacg ggacaggtcc tgcgctctcg
     1021 ctgggggcag gcgcaaacag gccgcccatc gtggtcaacg ccgccgcggg cacggcgacc
     1081 aacctcgacg cggaccagct cgacggccag gactccaccg acttccttcc tgccgagatc
     1141 tacgagaagt ccgcctccca gacgatatcg cctgactctt cggcaggcac gacggccacc
     1201 tgcgataccg gagacgtggc cgtgagcggg agctacgcca tattgggatc aaggctggac
     1261 gttacgagcg agcggcgcgt ggacgaccgt ttcgatgagg agacgggcca gaccaaccct
     1321 tctggctggc acgtgatcgc agagaatcca gacacgagtg cggaaggaac gataaccgtc
     1381 tacgtcaatt gcgccgaccg ggctcccgtg cattctccat agtacgcggc aatgaaagtc
     1441 catccggggt tgcttctaga tgggcggcga tagtagagtc ggggcgtagt atgtctatgt
     1501 cagcgagaag aatgaaggta tgaaatggtg gcgttcctga tcctgctgtt gttcgcggtt
     1561 atcttcctgg tagccggcgg cgtaatgctc tacttccgaa acagaaccaa gcagaagtcc
     1621 gccctcatga gccagaccga gacctcagcc tcggcggacg tctctggtct cgcacccggc
     1681 acgctggtcg aggtcaaggg aacgctgcgc tgcgaggagc ctcttacgag cgagatggcc
     1741 gaaaagagct gcgcctacta ctcctccaag gtaatacgcg agtacatgga gagagactac
     1801 gacgatgacg accacggcgg atcggacagg cgctctgaag tgatcgccca gaacgagcag
     1861 ttcgcgccgt ttttggtcga ggataccagc gggacggtgg ccgtgaacgc ggagggtgcc
     1921 gaggtagacg cccgtcaggt ggtggaccgc ttcgaaaggg acacggggaa cgagggttct
     1981 atcgccctcg gcggcatgac catcaacctc ggcgggggcg agcgcaccat cggctaccgc
     2041 tacaccgaga gtatcctacc catcgactca tctgtctacg tgctcggcac cgtgcaggat
     2101 ggaggtggga tcggggctcc ttcgtctggc gaagg
//
LOCUS       DATAIR010000157         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_378546, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000157 DATAIR010000000
VERSION     DATAIR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378546"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VHM69_03820"
     CDS             complement(<1..683)
                     /locus_tag="VHM69_03820"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX2739549.1"
                     /translation="MDLAVRKMTLERFGVENVGAVHENLPPARLVEASVRRREGMISG
                     SGALVVKTGKRTGRSPKDRFIVENEITRDTVDWGTGNKPFPSEAFGALLEKAATYVEN
                     LEEIYVVDAYAGADPRYSVNVQVVTEHAWQALFTRQLFRRPSKAEIDAFEPDWTVISV
                     PGLLTDPEEDATESETFIGIDFERKVVLVCGTAYAGEIKKSIFTVLNFVLPTNCDVFP
                     MHCSANAGQ"
     gene            877..>2169
                     /locus_tag="VHM69_03825"
     CDS             877..>2169
                     /locus_tag="VHM69_03825"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2739550.1"
                     /translation="MTQQINQETAGAEQWRALAQQLRADSIRSSSGAGSGHPTSSMSA
                     ADLIAVLMSKYLRYDFDGPENPNNDHLIFSKGHASPLLYSAYKAAGAISDEELMTFRE
                     FGSRLQGHPTPQIPWVDVATGSLGQGLPIGVGVALAGKYLDRLPYKVWVLCGDSEMAE
                     GSMWEAFQHASFYKLDNLIAILDMNRLGQTRETMDGWNGDHYEARARAFGWHTVQIDG
                     HDPEQIDRAYAEALEQTDTPTLIIAKTKKGRGVSFLEDVDGMHGKPVGPDQEQAALEE
                     LGGTNDLLVEVRKPDPGSDDNFRSGLTGELALPTWEVGDEEATRGAYGAALKALGSAR
                     EDVVALDGEVSNSTHSQEFAKEYPERYFEMFIAEQQMVAAAVGMSVRHRVPFASSFAA
                     FFSRAYDFIRMGAISGANIRLSGSHAGVSIGEDGPSQMA"
BASE COUNT          404 a          712 c          679 g          374 t
ORIGIN      
        1 ttctgtcccg cgttggccga gcagtgcatc gggaagacgt cgcagttggt cggcagcacg
       61 aagttaagca cggtgaagat gctcttcttg atctcccccg cgtaggcagt gccgcagacg
      121 aggaccacct tgcgctcgaa gtctatccct ataaacgtct ccgactccgt ggcgtcctcc
      181 tcagggtcgg taaggaggcc agggaccgag atcacggtcc agtcgggctc gaaggcgtcg
      241 atctcagcct tcgacggacg cctgaagagc tgcctggtga agagggcctg ccacgcgtgc
      301 tcggtcacca cctggacgtt cacgctgtag cgggggtctg cgccggcgta ggcgtcaacg
      361 acgtagatct cctcgaggtt ctcgacgtag gtggccgctt tttcgaggag cgccccgaac
      421 gcctcgctcg ggaagggctt gttgccagtg ccccagtcca cggtatcacg agtgatctcg
      481 ttctctacta tgaagcggtc cttcggggag cggcccgtgc gtttccccgt cttcacgacg
      541 agcgccccag aaccggagat cataccctcc ctcctcctca cggaagcctc gacaaggcgc
      601 gcgggcggca gattctcgtg cacggccccg acattctcaa ccccgaaacg ctccagcgtc
      661 atcttgcgaa ccgccaggtc catatatttc cctttcaccc agtcgaaccc gacgagtatg
      721 acggtcaggc ccgggttttg tcccttcgcc ttcgggattc taacaaagcc ggcataccgt
      781 cgtctgcacc tctggcacag gccgtttccg gatcggcggt gcggggtagg agcatataca
      841 atccgtttgg atggcgaatc tgccgaggag gtagtggtga cgcaacagat caaccaggag
      901 acagcaggtg ctgagcaatg gcgcgcgctc gcgcaacagc tgagggccga tagcattcgc
      961 tccagctccg gcgccggctc gggacatccc acctcgtcca tgtctgcggc agacctcatc
     1021 gcggttctga tgagcaagta cctgcgatac gacttcgacg ggccggagaa cccgaacaac
     1081 gaccacctga tcttctcgaa gggtcacgcc tcaccactgc tgtactcggc gtacaaggcg
     1141 gcaggcgcga tctccgacga agagctgatg acgttccgcg agttcggcag ccgcctgcag
     1201 gggcacccaa ccccgcagat cccctgggtc gacgtcgcca cgggctcgct aggccagggc
     1261 ctcccgatag gcgtcggggt ggcgcttgcc ggcaagtacc tggacaggct tccatacaag
     1321 gtctgggtgc tctgcggcga ctccgagatg gcggagggct cgatgtggga ggctttccaa
     1381 cacgcctcct tctacaagct ggacaacctc atagccatcc tggacatgaa ccgcctcggc
     1441 cagacccgtg agacgatgga cggctggaac ggcgaccact acgaggcgcg ggcgcgtgcc
     1501 ttcggctggc acaccgtcca gatcgacggc cacgaccccg agcagatcga ccgagcctac
     1561 gccgaggcgc tggagcaaac cgataccccg accctgatca tcgcgaagac caagaaggga
     1621 cgcggcgtct ccttcctgga agacgtggac ggcatgcacg gcaagcccgt cgggccggac
     1681 caggagcagg ccgcactcga ggagcttggc ggcaccaacg accttctggt cgaggtgcga
     1741 aagcccgacc ctggctcgga cgacaacttc agatccggcc tgaccggcga gctggcgctg
     1801 ccgacctggg aggtcggtga cgaggaggcc acccgtggcg cgtacggcgc ggccctcaaa
     1861 gcacttggct ccgcaaggga agacgtcgtc gcgctcgacg gcgaggtgag caactcgacg
     1921 cactcccagg agttcgccaa ggagtatccc gagcgctact tcgagatgtt catcgccgag
     1981 cagcagatgg tcgcggctgc cgtggggatg agcgtgcgcc acagggtacc gttcgcatcg
     2041 agcttcgccg ctttcttctc ccgcgcctac gacttcatca ggatgggcgc tatctcgggc
     2101 gccaacatcc gcctctcagg ctcgcacgca ggcgtctcca tcggtgagga cggtccgtcg
     2161 cagatggcc
//
LOCUS       DATAIR010000158         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_286696, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000158 DATAIR010000000
VERSION     DATAIR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286696"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHM69_03830"
     CDS             <1..292
                     /locus_tag="VHM69_03830"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine phosphoribosyltransferase family protein"
                     /protein_id="HEX2739551.1"
                     /translation="PNKLPRETISATYELEYGTDALEAHADSIPEGTRILVADDLLAT
                     GGTARAMCQLVENAGGIVAGVAFLIELGYLEGRDQLRGYDVVSLITYHDPDD"
     gene            285..1238
                     /locus_tag="VHM69_03835"
     CDS             285..1238
                     /locus_tag="VHM69_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2739552.1"
                     /translation="MTNLPQSLEDTTPSPQPVPPQAERLIAVYLRTLTSPQTIKTYNT
                     ELTLFLSYLEQKMGKRLEDLTAEDISLYREHLINNYAAATAAKKLAVLRRFLMFTYMA
                     GATTVNPESLRFFAKSPRVRQDPAYNVLTEDELSRMLNAARASNYRDYVMLAVMAACG
                     LREAEVVALKIGDFKEAGADQVLLRVLGKGDKVRNVSISPDLWRLVQRYVLLTERSFT
                     SHPDSRKPLFTSRVGKNKALTTRSVQYMVKKYVRAAGITKAISPHSIRHTVGTNMAVN
                     EAPLLVIQQFLGHSDPKTTMRYIRRAEELATRAYQYNTLPM"
     gene            complement(1267..2778)
                     /locus_tag="VHM69_03840"
     CDS             complement(1267..2778)
                     /locus_tag="VHM69_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2739553.1"
                     /translation="MPVRWRLTVFNALVIGTILTLLGVSVFLLLRDALLSGVEYTVRD
                     RAASVARTVEMGQSLSAGDIDQLTLEGVFVVVRDSEGRILARTVNTGPAGGPGRPFWQ
                     TAVESGEAVDGQAELSPGERGYVYAVPVDPPDLGNLSRSPYLYKALTRDRRATDSKSA
                     PVLGEAARIPFPVEARVIEVGKSYEAAGETVSTFAVLLAAVIFIALLISAGGAYLLAH
                     AALSPVERVVASARGITAGDLSKRVPVQHPKDEVGRLAATINDLLGRLEAAFARREEA
                     LAHQRRFVADASHQLRTPLTSIEGYARMLAEWGFDDPATTREGAATIHREARHMKKLV
                     QDLLSLARGDEGAPLNPQVHNLDALAANASKLAANGKAPIRHTPATEPVLASFDRDRI
                     EQAVSILVENAVKYSPEGGEIVLNASQHNGTVRIEVSDAGMGIPSEHIPLIFERFYRS
                     DAARPMRGAGLGLSIALQIAEAHGGTLAVESEVGKGSTFTLQLPKDGPVRPES"
     gene            complement(2780..2920)
                     /locus_tag="VHM69_03845"
     CDS             complement(2780..2920)
                     /locus_tag="VHM69_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2739554.1"
                     /translation="MWGHESGVETNVVDVYVGYLRKKLEVAGEEALIRTVRGAGYALR
                     EN"
BASE COUNT          542 a          965 c          873 g          549 t
ORIGIN      
        1 gccgaacaag cttccgaggg agaccatctc cgctacctac gaactcgagt acggcacgga
       61 cgccctggag gcccacgcag actcgatccc ggaaggtacc cgcatcctcg tcgcagacga
      121 cctgctcgcc accggcggaa cggcccgcgc gatgtgccag ctcgtcgaaa acgccggggg
      181 tatagtcgcc ggcgtggcct tccttataga actcggctac cttgaaggtc gcgaccaact
      241 ccgcgggtac gatgtggtct ccctcattac ctaccacgac ccggatgact aacctcccac
      301 aatcgctaga ggacacaact ccatctcccc agcccgtccc cccacaagcc gaacgcctca
      361 tagcagtcta cctgcgtacc ctcacaagcc ctcagacgat aaaaacctat aatacagaac
      421 tcacgttatt cctctcctat ctcgagcaga agatggggaa gaggcttgag gatttgactg
      481 cggaggacat ctcactgtat cgggagcacc tgatcaataa ttacgcggcg gccacggcgg
      541 ccaagaagct cgcggtgctc aggcggttct tgatgttcac gtacatggcc ggggcgacga
      601 cggtgaaccc tgagtcgttg cgcttcttcg ccaagagtcc gcgcgtacgc caggaccctg
      661 cctacaacgt gctgaccgag gacgagttgt cgcgcatgtt gaacgccgcg cgtgcctcca
      721 actaccgcga ctacgtcatg ctcgccgtca tggctgcatg tggtttgcgg gaggccgagg
      781 tcgttgcatt gaaaatcggg gacttcaagg aggccggtgc agatcaggtc ctcctccgcg
      841 ttctaggcaa gggtgataag gtgcgcaacg tctcgatctc acccgacctc tggcggctgg
      901 tccagcgcta cgtcctgctg acagagcgca gcttcacctc gcacccggac tcgcgcaagc
      961 ctctattcac ctccagggtc ggcaagaata aagcgctcac cacccgctcc gtccagtaca
     1021 tggtgaagaa gtacgtgcgg gccgcgggca tcaccaaagc catctcaccc cactccatcc
     1081 gccacacagt gggtaccaat atggccgtaa acgaagcgcc cctcctcgtc atccagcagt
     1141 tcctcggcca ctcggacccg aaaacgacca tgcgctacat ccgccgtgca gaagagctcg
     1201 cgacccgcgc atatcaatat aatactctac cgatgtagga aggtgggggt gtgaactccc
     1261 tgtcgcctaa ctttctggcc ttaccgggcc gtccttcggg agttgcaggg tgaaggtgga
     1321 gcccttgccg acctcgctct cgaccgcaag agttcctccg tgggcttcgg cgatctggag
     1381 cgctatcgag aggccgagtc cggcaccgcg catcgggcgc gccgcgtccg acctgtagaa
     1441 ccgttcgaag atcaaaggta tgtgctcgct tgggataccc atacccgcgt cggagacctc
     1501 gattctaacc gtgccgttgt gttgggaggc gttcaagacg atctcacctc cttcgggcga
     1561 gtatttcacg gcgttctcga caaggatgga cacggcctgc tcgatgcggt ccctgtcgaa
     1621 gctggcgagc acgggctctg tggccggtgt gtggcgtata ggggccttgc cgttcgccgc
     1681 gagtttcgac gcattggctg cgagggcgtc caggttgtgt acctgcgggt tcaacggggc
     1741 gccttcgtcg cccctcgcca gggagagaag gtcctgtacc agcttcttca tgtgtcgcgc
     1801 ttccctgtgg atcgtcgccg cgccctcacg cgtcgttgca gggtcgtcga atccccactc
     1861 ggcgagcata cgagcgtagc cttcaataga ggtaaggggc gtgcggagtt gatggctcgc
     1921 gtctgcgacg aagcggcgct ggtgcgcgag tgcctcctct cggcgggcga aggctgcttc
     1981 cagcctgccg agaaggtcgt ttatagtggc tgcgaggcgc ccgacctcgt ccttcgggtg
     2041 ctgaaccggc acccgcttcg agagatctcc cgccgtgata ccgcgcgccg aagcgactac
     2101 cctctccaca ggagagagcg ccgcgtgtgc gagcaggtag gcgccgcccg ctgatatgag
     2161 gagggctata aagatcaccg cggcgagcag cacggcgaac gtcgagaccg tctcgcctgc
     2221 cgcctcgtat gacttgccca cctcgatcac ccgtgcttcc accgggaagg ggatcctggc
     2281 cgcctctccg aggactggcg cgctctttga atcagtcgcc cgtctgtctc gtgtgagcgc
     2341 cttgtagagg taaggtgagc gagataggtt cccgaggtcg ggagggtcta cgggcaccgc
     2401 gtagacgtag cccctttcgc cgggcgacag ctcggcctgt ccgtctaccg cttcgccgct
     2461 ctcgacggca gtctgccaga agggtctccc tgggccgccc gccggacccg tgttcacagt
     2521 gcgggcgagg atcctgccct cgctgtcacg cacgaccacg aacacgccct ccagggtgag
     2581 ctgatcgatg tcgccagcgc tgagcgattg tcccatctcc accgtgcggg ctacgctcgc
     2641 ggcacggtcc cgcaccgtat attcgacccc ggagagcagg gcgtcgcgca ggaggaggaa
     2701 gacggagacc ccgagcaagg tgagtatcgt gcctattaca agggcattga agaccgtaag
     2761 gcgccacctt acaggcatgc tagttttccc tgagcgcgta ccccgcgccc ctgacggtcc
     2821 ggatcaaagc ctcctcgccc gcgacttcca gcttcttgcg caggtacccg acgtagacgt
     2881 ccacgacgtt ggtctccacg ccggattcgt gcccccacac cctcgatag
//
LOCUS       DATAIR010000159         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_378622, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000159 DATAIR010000000
VERSION     DATAIR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378622"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(577..1158)
                     /gene="lepB"
                     /locus_tag="VHM69_03850"
     CDS             complement(577..1158)
                     /gene="lepB"
                     /locus_tag="VHM69_03850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2739555.1"
                     /translation="MSDKPYYHEQFQTSRQERRRASKKGGGALEYLVILLVSFALVFG
                     FVRPFVMEAFWIPSGSMIPTLEIGDRVLVNKFIYRFTEPTRGDIVVFESVDNPEEDLI
                     KRVVGLPGDRIAVRNGRLFVNGEPQKEPYTNKRLPDRSFFAQVTVPKDHIFVMGDNRA
                     NSADSRVFGPLPEENIEGEAFVRFWPPDRIGLL"
     gene            complement(1213..1983)
                     /locus_tag="VHM69_03855"
     CDS             complement(1213..1983)
                     /locus_tag="VHM69_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2739556.1"
                     /translation="MDYLEFLILGVIGVIGGMLSGLVGVGGGIVFVPGLMYAGGWHIQ
                     EAVAASLVIIVFSSLSGTLRNARSSDPVNWRVAGLLSLAVAPSSLIGVFISRVSPDMV
                     VKVAFATLLIALAYPTARGGGEYDGNRKSIPLPLVFLAGIFTGTLSGLVGVGGGVVMV
                     PLMVLGMGLSTKKAVSTSLAVVMFTGIIGAAGYVATGFRDPQYLLSLPPLILGSIIGA
                     PLGVRIRDWLPENVVRIGFGVFMVIVALRLFGEALGVF"
BASE COUNT          446 a          600 c          659 g          348 t
ORIGIN      
        1 gtggctggac gacagacacg cggtcttcgg ggaggtcatc gaaggtatgg atgtggtgaa
       61 cgccatcaag gagcgcgacc cgcagcgcaa ccgcgagccc ggcgacaaga tagagcgggt
      121 cgagatcgaa gaggtccccc ggtcctgatt ccacccttga gtgcgcattc acagtttgtc
      181 cctagggtcg cacgataccc tcgtcgggca aacgagatcg cacgagagag gtggtcctgt
      241 gtccatagac gtagcggaca ggttcgtgga ggcactctgg aagatggcag gatcaggcgt
      301 ttccgtgcct acttcgaccc ccgcgccgtc agcgaaaagg tggttgatta ggtttcaggt
      361 attaggcatc aggtttcagg ctacagggct tgttgcggga aggaatttcg tttgctggat
      421 gggatgtccc gaggcgatgg atcaacccgt ccgacgggga gaaggtggcg gaccggcgag
      481 atcagggtcg ggcaagcgcc gctctgacgc gagagaccct ctccgtccac cgagggccga
      541 cagctgatgc ctgaaatctg atgcctgtag cctctatcag agcagcccga tacggtcagg
      601 cggccagaag cgcacgaaag cctcgccttc tatattctcc tcgggcaggg gtccgaagac
      661 gcgggagtcc gctgagttag cgcggttgtc gcccataacg aagatgtgat ctttcgggac
      721 cgttacctgt gcgaagaagc tcctgtccgg gagcctcttg ttggtgtaag gctccttctg
      781 aggctcgccg ttcacgaaga gcctgccgtt gcggacggcg atcctgtcgc cgggcagtcc
      841 gacgacgcgt tttataagat cctcttcagg gttatcgacg ctctcgaaga cgacgatgtc
      901 gccgcgcgta ggttccgtga aacggtagat gaacttgttt accagcacgc gatcgccgat
      961 ctcgagcgtc gggatcatgc tccccgaggg gatccagaag gcctccatga cgaaggggcg
     1021 gacgaagccg aagaccagcg cgaaggagac gagcagtatg accaggtact cgagcgcacc
     1081 cccgcccttc ttggaggcac gccgacgctc ctgcctggag gtctggaact gttcgtggta
     1141 gtaaggctta tcggacatga agcattagga tatcgcaccg ggacggatat ggctgaaaaa
     1201 atcctgaatt cctcaaaata cgccgagcgc ctctccgaag aggcggaggg ccacgatcac
     1261 catgaagacg ccgaagccga tccgtacgac gttctcgggc agccagtcac ggatgcgcac
     1321 cccgagaggg gccccgatta tcgacccgag tatcaggggc ggtagactga ggagatactg
     1381 cgggtccctg aagcccgtcg ccacgtagcc cgccgccccg ataatcccag tgaacatcac
     1441 gacggcgagg ctcgtcgaga ctgccttctt cgtggacagg cccatgccga gcaccatcag
     1501 cgggaccatc accaccccgc cacctacccc gacgagccct gagagcgtcc ccgtaaagat
     1561 gcccgcaagg aagacgagcg ggaggggtat gctcttgcgg ttgccatcgt actcgccccc
     1621 gccgcgggcc gtcgggtagg cgagggctat gaggagcgtg gcgaaggcaa ccttcacgac
     1681 catgtcgggg gaaacgcggc ttatgaacac ccctatgagg gacgatgggg cgacggcgag
     1741 ggagaggagg ccggcaaccc gccagttaac agggtcggag cttctggcgt tgcgtaacgt
     1801 gcccgagaga gagctgaaga ctatgatcac caggctggcg gcgacggcct cctggatgtg
     1861 ccatccaccc gcgtacatta gaccaggcac gaagacgata ccgccgccaa cgccgacgag
     1921 accggagagc atacccccga tgacgccgat aaccccaagg atcaggaact caagataatc
     1981 catgctagct taacgccgcg ctaggacgcg gagcgggact ttctccagag ccagaacaga
     2041 tagactgccc cgg
//
LOCUS       DATAIR010000160         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_425083, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000160 DATAIR010000000
VERSION     DATAIR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425083"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHM69_03860"
     CDS             <1..281
                     /locus_tag="VHM69_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2739557.1"
                     /translation="SLEDIRRQAGDAFDIRVPQGRRLPFSPLAKKSLERALRESVRLR
                     DGRIGTEHILLGILRNEEGMAVRMLARMGVSPGALEDRLFELRGRATG"
     gene            501..1217
                     /locus_tag="VHM69_03865"
     CDS             501..1217
                     /locus_tag="VHM69_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739558.1"
                     /translation="MEDPTTEEFMIEATGVQKVYASNGLRVHALKGLDLGIRRGEMVA
                     IMGPSGSGKTTLLNCLSGLDDLDGGEVLIAGDPISKMSDRKRTRFRAQRMGFIFQSYN
                     LIPVLSAVENIELPLLVAGVKPKAARIRAEEALEMVGLADQTKKRPAQMSGGQQQRVT
                     VARSLVNEPDIVWADEPTGALDSETSKEIMDLLVRLNEEKGQTFVLVTHDAAVAARAH
                     RTIRMRDGRIASDAPNGKKG"
     gene            1221..4211
                     /locus_tag="VHM69_03870"
     CDS             1221..4211
                     /locus_tag="VHM69_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2739559.1"
                     /translation="METLFGVPTQQLMWVLLAVFGAGALILILSALRNRVAFKMAVRN
                     IPRRKSQSVLIVIGLMLATMLFSASFTTGDTLTNSLRVQALQNIGQVDVAIKPDAPES
                     AGQFAGPAARPTYFDQDVTQDVREDLAGDEKVAGVAPLISETVPVLSAESDLSEPQAD
                     VLGIDEASMQAFDHPTTASGTALSLDDLSKNEVYISSETAKNLDVKKGDEVEAVLGAR
                     PAKLEVAGIYESGANPASDTSMVMPLAELQKLTGNEGRINQVIITHKGPSVEGGAHTS
                     ATVEALKPLLKENDLRADPLKKDAMKQADDVGKSISSIFLLFGQFSTAAGILLIFLIF
                     VMLAAERKHELGIARGVGMRRGHLMRMFAFEGALYALVASAIGSVAGVGVGWVMVKVI
                     GKAFAQFDEFSFQIAFATSPENVIIAFSLGMVLTFLIVLLSAWRVSRLNVVRAIRDIP
                     EPETQGRSVKGILLALATPLAGAFLVWQGLQVDQMGLYMLGLSLLIVGAALIARILRL
                     PDRISFSLAGLGLLALWLLPYDFAPPEMTQGIDLFFISGIMIVIGAVWVVIYNSDLLL
                     GAVVAVLGRIRGLPPVLKTAVSYPMQSRFRTGMTLAMFSLVVFTIVTMSFITTSISGI
                     YDDTDRLSGGFDIRADAGYTAPVSDMESALKNTKGVDSDDISAVGATSSLSVKVKQEG
                     TDRKMKDLVVQGADEGYTQNVGYGFNVTAAEYDSSRDVWNALQKERNVAVISASIAPS
                     RSSFDVGDEEPPLKLSGFYQEDKALPDDLYIHVRDPKSGEERDLRVIGVVDDAAFYVG
                     NIMTSQKTLDALAGAPVPPQSYMFELADGASAPATARDLEEGFARNGLQATVLEEEIL
                     DSASTNNILNNLLTGFMGLGLLVGIAALGVIAARSVVERRQQIGMLRALGFQKGQVRL
                     AFLLESSFIALLGIGLGVALGAALSGGLIDSLSEDIAGVTYKVPWFALALVVALSYAA
                     SLLTTFLPARGASKVYPAEALRYE"
     gene            4339..>5118
                     /locus_tag="VHM69_03875"
     CDS             4339..>5118
                     /locus_tag="VHM69_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2739560.1"
                     /translation="MGEIFALRRLRWKLTLSYTLVAAVTLLALELILAGLVVAFLNSP
                     LLPRIVVQNVRDEVAPRLEKPLNQNPPDVEGIREELGAIADPRGIGRGPGPPTFANYT
                     VDQGALFVVDDRRRLLVSAPEIQDFPEGRHFDAGSITGLKPLVNAAFDGEENSGRLSS
                     NSDGRVLSVTPIEGKKGHIAGVLVFTLRMPNLTGPLLVAIGASATLLTIPAAFLGALF
                     GFFTAWGLTRRIGRLARAARSWSRGDFSVTTKDRSKDELGQL"
BASE COUNT          941 a         1652 c         1590 g          935 t
ORIGIN      
        1 tctccctcga ggacatccgc aggcaggcgg gtgatgcctt cgatattcgc gtaccccaag
       61 gacgccggct tccgttctca ccgctggcga agaagtctct cgagcgggcg cttagggagt
      121 cggtgcgcct ccgagacggc cgcatcggaa ccgagcacat cttgctaggc atactgcgca
      181 acgaagaagg gatggcggtc aggatgctgg cccgtatggg ggtttcaccc ggggcgctgg
      241 aggacaggct cttcgagttg cgggggcggg cgacaggcta atgggttgga gcgattagtt
      301 tccaactacg ccggcgcatt cggccaccat cctcggaggt cgagatcaca tccgtctacg
      361 gacctagcca acttcaaccc tccgcccgat gtacgcatcg agcaaccagc ctatcgttac
      421 tgcgtagtgt gtagacgacc gaggagaaaa tcgtgcccga gcggagagag cctgtttcta
      481 ccgaacgagg aggaagtctc gtggaggatc ccacgacaga ggagttcatg atcgaggcca
      541 ccggcgtgca gaaggtgtac gcctcaaatg gccttcgggt gcacgcgctc aaagggttgg
      601 accttgggat ccggcgcggg gagatggtcg ccatcatggg accgtcgggc agcggcaaga
      661 cgacgctgct caactgcctt tcgggtctcg acgatctgga cgggggcgag gtgctcatcg
      721 caggagatcc gatcagcaag atgtcggaca ggaaacggac ccgcttcagg gcccagagga
      781 tgggcttcat cttccagtcg tacaacctga tcccggtcct ctccgcggtc gagaacatcg
      841 agctgcctct gcttgtggca ggcgtgaagc cgaaggcggc ccgcatccgc gccgaggagg
      901 cgctggagat ggtggggctc gcggaccaga cgaagaagcg tcctgcccag atgagcggcg
      961 gccagcagca gcgcgttacg gtcgcccgct cgctggtcaa cgaacccgac atcgtatggg
     1021 ccgacgagcc gacaggcgcc ctcgactctg agacgtcgaa ggagatcatg gacctcctcg
     1081 tacgcctgaa cgaggagaaa ggtcagactt tcgtcctcgt cacccacgac gccgccgtcg
     1141 ccgcccgcgc ccaccgcacg atccgaatgc gcgacggccg catcgcgagc gacgcgccga
     1201 acgggaagaa aggctagaag atggagactc tcttcggcgt tccgacgcag cagctcatgt
     1261 gggtcctcct cgccgtcttc ggcgcgggcg ccctgatcct gattctttca gccctccgca
     1321 accgggtcgc cttcaagatg gccgtaagga acattccccg acgcaagagc cagtcggtcc
     1381 tcatagtgat cggcctgatg ctcgcgacca tgctcttctc cgcctctttc acgacaggcg
     1441 acaccctgac gaactccctc agggtccagg cgctccagaa catcggccag gtggacgtgg
     1501 cgatcaaacc agacgcaccc gaatctgcgg gccagttcgc gggaccggcc gcgcgcccga
     1561 cctacttcga ccaggacgtc acacaggatg tcagggaaga ccttgccggc gacgagaagg
     1621 tagccggggt cgcgcccctg atctccgaga ccgtcccagt gctctcagca gaaagcgacc
     1681 tctccgagcc gcaggccgac gttctcggca tcgacgaagc ctcgatgcag gctttcgacc
     1741 accccactac cgcgtcaggc acggcgctct ccctcgacga cctctccaaa aacgaggtct
     1801 acataagctc tgagacggcg aagaacctgg acgtgaagaa gggagacgaa gtagaggcgg
     1861 tcctcggcgc gcggcctgcg aagctcgagg tcgcaggcat atacgagagc ggcgcaaacc
     1921 ccgcgtcgga cacgtcgatg gtcatgccgc tcgcagagtt gcagaagctc acgggcaacg
     1981 aagggaggat caatcaggtt atcatcaccc acaaggggcc ttctgtcgag ggcggagcgc
     2041 acacgagcgc gacagtcgag gcgttgaaac cgcttctcaa ggagaacgac ctcagggcgg
     2101 atcccctgaa gaaagacgcc atgaagcagg ccgacgacgt cgggaagagc atctccagca
     2161 tcttcctgct cttcggccag ttctccaccg ccgcggggat acttttgatc ttcctgatct
     2221 tcgtgatgct cgccgccgag cgcaaacacg agctgggtat cgcgcgcggt gtgggcatgc
     2281 ggcgcgggca tctgatgagg atgttcgcct tcgagggcgc actctatgca ttggtagcct
     2341 cggccatcgg cagcgtggcg ggtgtcgggg tcggttgggt gatggtaaag gtcatcggga
     2401 aggccttcgc gcagttcgac gagttcagct tccagatagc gttcgccacc agcccggaga
     2461 acgtgatcat cgcgttctcg ctcggcatgg tcctgacctt cctgatcgta ctcctctccg
     2521 cctggcgcgt aagccgcctg aacgtggtca gggccatccg cgacatcccg gaacctgaaa
     2581 cacagggccg cagcgtgaag ggcatccttc tcgcgctcgc gaccccgctc gccggggcat
     2641 ttctcgtatg gcaggggctc caggtcgacc agatgggcct ttacatgctt ggactttcgc
     2701 tcctgatcgt cggggccgcg ctcatcgcgc gcatcctgcg cctgcctgat aggatctcgt
     2761 tctcgctcgc cggcctcggg ctgctcgccc tgtggctcct gccctacgat ttcgcgccgc
     2821 cggagatgac gcaggggatt gatctcttct ttatctcggg gatcatgatc gtcatcgggg
     2881 ccgtgtgggt ggttatctac aactctgacc tgctgctcgg cgccgtcgtc gccgtcctcg
     2941 gacgcatccg gggccttcct ccggtcctga agaccgccgt ctcgtacccg atgcagagcc
     3001 gcttccgcac gggcatgacg ctcgcgatgt tctcgctggt cgtcttcacc atcgtgacga
     3061 tgagctttat cacgacgtcg ataagcggca tctacgacga cactgaccgg ctctccggcg
     3121 gtttcgatat ccgcgccgac gcaggctata cggcccccgt ctcggacatg gagagcgccc
     3181 tgaagaacac gaagggcgtg gactccgacg atatctccgc agtcggcgcc acctcaagcc
     3241 tctctgtgaa ggtcaagcag gaggggacgg acaggaagat gaaggacctc gtcgtacagg
     3301 gcgccgacga agggtacacg cagaacgtcg gctacggctt caacgtcacc gccgctgaat
     3361 acgactcgtc tcgggacgta tggaacgccc tccagaagga acgcaacgtc gcggtgatct
     3421 ctgcctccat cgcgccttcg aggtcgagct tcgacgttgg tgacgaggag cctcctctca
     3481 agctcagtgg cttctaccag gaggacaagg cgcttcccga cgacctctac atccacgtca
     3541 gggacccgaa gtccggcgag gagcgggacc tgcgggtgat cggggtcgtc gacgacgcag
     3601 cgttctacgt aggcaacatc atgacctcgc agaagacgct cgacgcttta gccggcgctc
     3661 ccgttccacc ccagagctac atgttcgagc tcgctgacgg cgcgagcgcc ccggccacgg
     3721 cgagggacct cgaagagggc ttcgcccgca acggtcttca ggccacggtt ctcgaggagg
     3781 agatcctgga ctcagcatcg acgaacaata tcctgaacaa cctgctcacg ggctttatgg
     3841 gccttggact cctcgtcggc atcgccgccc tcggtgtcat cgcggcccgc tctgtcgtcg
     3901 agcgcaggca gcagataggg atgctcaggg ctctcggttt ccagaagggc caggtgcgcc
     3961 tcgctttcct cctcgagtcg tcgttcatcg ccctgctcgg catcgggctc ggcgtcgccc
     4021 tcggcgcggc gctctcgggc ggcctcatag actccttgag cgaggacatc gccggcgtca
     4081 cctacaaggt gccatggttc gccctggcgc tcgtcgtcgc tctctcctac gccgcttcgc
     4141 tcctcaccac cttcctcccg gcccgcggag cctcgaaggt ctaccccgcc gaggcactgc
     4201 gctacgagta gagagctaat ggagggtccg ctcggaccct ccatttcgtt ccatggaccg
     4261 accctcctta cgtcgcccga ctgatgcgct ctgtcgcatg cgggactaaa cttgccgtaa
     4321 tcatgagaaa cagtgatcat gggtgagatc ttcgcgctcc ggcggctgcg gtggaagctg
     4381 acgctgagct atacgctcgt ggccgcggta acgctgctcg ctctggagtt gatcctcgcg
     4441 ggcctcgtgg tcgcattcct taattctccg ctcctgccga ggattgtggt tcagaacgtc
     4501 agagatgaag tagcgcctcg ccttgagaag ccactcaacc agaacccccc tgacgtcgaa
     4561 ggcatacgag aggaacttgg agcaatcgct gaccctcgtg gtattggccg cggaccgggc
     4621 cccccgacct tcgccaacta tacggtggat cagggcgccc tcttcgtcgt cgacgacaga
     4681 cgccgtctat tggtctccgc ccctgagata caagacttcc ctgaggggag gcacttcgat
     4741 gccgggagca tcaccggctt gaagccactc gtgaacgcgg cgttcgacgg cgaggagaac
     4801 tcgggccgcc tctcttcgaa ctccgatggg cgggtgctca gcgtcacgcc catcgaggga
     4861 aagaaagggc acatcgccgg cgtacttgtt tttacgttgc ggatgccgaa ccttacgggt
     4921 ccgctcctcg tagccatcgg tgccagcgcg accttactga cgataccagc cgccttcctg
     4981 ggtgccctct tcggcttctt tacggcctgg ggcctgacgc gtcgcatagg gcgtctcgcc
     5041 agggcggccc ggtcgtggag ccggggcgac ttctcggtga cgacgaaaga ccgctcgaag
     5101 gacgagctcg gccagctc
//
LOCUS       DATAIR010000161         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_9846, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000161 DATAIR010000000
VERSION     DATAIR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9846"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VHM69_03880"
     CDS             complement(<1..114)
                     /locus_tag="VHM69_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739561.1"
                     /translation="MTDRNQELVTPEGLEKLQKELAYLTEVRRKEVADRIRQ"
     gene            complement(326..1090)
                     /locus_tag="VHM69_03885"
     CDS             complement(326..1090)
                     /locus_tag="VHM69_03885"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2739562.1"
                     /translation="MAVDVGNTQTVLGLFDGKEPRGHWRIATEVHRTSDELAVFCAGL
                     LALEGLRLDDVSAMVVSSVVPGLTSSYRHLASENFGVPFYAVTAEMETGLDNRYDDPR
                     AVGADRIVNAVAAGRQYGFPAIIVDIGTATTVEAVNGEGCYLGGAILTGLYVSLEALV
                     SRTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAVDALIRRSREELGEDGVRVV
                     ATGGQAGVIVPYCQEIQEFDPDLTLKGLLALYEMNA"
     gene            complement(1090..1593)
                     /gene="folK"
                     /locus_tag="VHM69_03890"
     CDS             complement(1090..1593)
                     /gene="folK"
                     /locus_tag="VHM69_03890"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2739563.1"
                     /translation="MSRTFVSLGSNLGDRLGYLRAAVSALRRGPGMRVTDTSKVYETA
                     PVEVEEEQPDYLNCVVELECGLPAVELLRYCQGIEGALGRERGEAGEKAPRTLDIDVL
                     LFGEETIEKTELQVPHRGVMRAFNLTGLADLDVDLYIPDLGVVAELLADVEGGGIRVF
                     GEAEELC"
     gene            complement(1590..2402)
                     /locus_tag="VHM69_03895"
     CDS             complement(1590..2402)
                     /locus_tag="VHM69_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2739564.1"
                     /translation="MFERFTERARKVMVLAQDEARHFNHNYIGTEHILLGLVSEGRGV
                     AAQSLSSLGVTLGAVRDQVESIIGYGEDSGRQIPFTPRSKKVMELGLRESMQLGHNYI
                     GTEHILLGLVREGEGVAARVFSNLGIAPGRVRREVLRRLGEETEPEPVGETEAEAAGN
                     RMAFRGRVDSLQVGGRVGGRAMTLLVGLDYEYEVRDTDEPSEALNHGDLLGGVVEVFE
                     GSDLGSVEAGILEAGRLVLDGFPAVHEVVIGATRELEGRAATGITVNRTFRR"
     gene            complement(2443..3315)
                     /gene="folP"
                     /locus_tag="VHM69_03900"
     CDS             complement(2443..3315)
                     /gene="folP"
                     /locus_tag="VHM69_03900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2739565.1"
                     /translation="MSRTPAARNTPGDGTFLPGPVLVGILNVTPDSFSDGGEFLDPEP
                     AANHAAAMLDEGAGIIDVGGESTRPGSDPVAQDEEIRRVIPVIERLLAVRPEAVISID
                     TYRAETAAAALGAGASVVNDVTALRGDPRMASFVAEAGCPVVLMHMKGEPKTMQEEPF
                     YDDVVREVRDFLAQRAEYAVAAGVRPENIIVDPGIGFGKSLEHNLALLRDLDAIVDLG
                     FPVIVGASRKRFIGTITGVEVASERVFGTVATTVLAYQKGARYFRVHDVRANRDALAV
                     AEAVLRRSEGSRRR"
     gene            complement(3316..>4374)
                     /locus_tag="VHM69_03905"
     CDS             complement(3316..>4374)
                     /locus_tag="VHM69_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2739566.1"
                     /translation="GHDEREQTLNQLLVEMDGFDSKSGIIMLAATNRADILDPALLRP
                     GRFDRQIVVDRPDLPGREKILRVHTRGKPLGPDITIDTLARGTPGFTGADLANLVNEA
                     ALLAARHDKDQIDMAEMEEAVDRVIAGPERKTRLISEKEKEITAYHEAGHAIVGALLP
                     EADPVHKITIIPRGQALGITMSLPTEDRFMMSRGQLMAQLAMMLGGRAAERVVFEEIT
                     TGASNDLERVTQTARQMVTRFGMSEKLGPMALGHQQGQVFMGRDFNNQPDYSDEIAFQ
                     IDKEIRRIVDESYDTAEDLLIRNRELLNKLSAELIEYETVDAKHLKRLIEEYAVDGVS
                     PNGALPDSDGHHRNRYLD"
BASE COUNT          717 a         1458 c         1343 g          856 t
ORIGIN      
        1 ctggcggatg cggtcggcga cctctttgcg gcgcacctcg gtgaggtatg cgagttcctt
       61 ctggagcttc tcgagaccct caggggtaac gagctcctgg ttcctgtccg tcataagccg
      121 cgagattata agggcgggtg cgaagggtgt caacgaaaat aggtttcagg tttcaggctt
      181 cagcttttgt ggtgcgaggg gaaatggtgt tcgaggagta gttccgtcgt gtctggcgca
      241 cctctctgag aggttgcttg atgcgcggga cgagcctcac atgcgccgcg aggacactac
      301 ctgatgcctg aaacctgtag cctattcacg cattcatctc gtatagcgcg aggaggcctt
      361 tcagagtgag atcagggtcg aactcctgga tctcctggca gtagggtacg atgacgccgg
      421 cctggccgcc ggtcgcgacg accctgacgc cgtcctcacc cagctcctcg cgggagcgtc
      481 ttatgagggc atccacggcg cctgcgtagc cgtagatgaa accgctgcgg atggagtcgg
      541 gggtgttggt ggctatgacc ctcggcggtt cttcttcgag gtcaacgctc gggagcttgg
      601 cggtcctgga gacgagcgct tcgagtgaga cgtagaggcc cgtcagtatg gcgccaccga
      661 ggtagcatcc ttcgccgttc acggcctcga cggtcgtcgc cgtgccgatg tccacgatga
      721 tcgcggggaa cccgtattgc ctccctgccg ccactgcgtt gacgatcctg tccgccccga
      781 ccgctctcgg gtcgtcatag cggttgtcga ggcccgtctc catctccgcg gtgaccgcgt
      841 agaagggaac cccgaagttc tcggaggcga ggtgccggta cgagctcgtg agaccgggca
      901 cgacgctgga gacgaccatc gcgctcacgt catccaggcg cagcccctcc agagccagga
      961 gcccggcgca gaagacggcg agctcgtcgg aggtgcgatg cacctccgtg gcgattcgcc
     1021 agtgtccccg cggctccttc ccgtcgaaga gccccagtac cgtctgcgta ttcccgacgt
     1081 ccacagccat caacaaagct cttcagcctc cccgaacacc cggatcccgc ctccttctac
     1141 gtccgcaagc agctccgcga ccacgccgag gtccggaata tacaggtcca catccaggtc
     1201 agcgagcccc gtgaggttga aggcgcgcat aacccccctg tgcggcacct gaagctcagt
     1261 cttctcgatg gtttcctccc cgaacagcag cacgtctatg tccagggtac gtggggcctt
     1321 ctcgcccgct tcgccgcgct cgcgcccgag ggcgccctcg atcccctggc agtagcggag
     1381 caactcgacc gcgggaagac cgcattccaa ttcgaccacg cagttcagat aatccggttg
     1441 ctcttcttct acctcgaccg gtgccgtctc gtagaccttc gaagtatcgg tgaccctcat
     1501 ccctggacct cgccgcaaag ccgataccgc cgcccgcaga tacccgaggc ggtcgcccag
     1561 gttgcttccc agactgacga aggtcctgct cacctccgga aggtgcggtt gaccgtgatg
     1621 cccgtggccg cgcgcccttc gagctccctg gtcgcgccga tcacgacctc atgtacggcg
     1681 gggaacccgt ccaggaccag cctgcccgcc tccagtattc cggcctcgac cgatccgaga
     1741 tcgctccctt cgaagacctc cacgacaccg ccgaggaggt cgccgtgatt cagggcttcc
     1801 gaaggctcgt ctgtgtcgcg tacctcatac tcgtaatcca ggcccacgag cagcgtcata
     1861 gcccgtcccc cgacgcgtcc gcctacctgc agcgaatcca cccgtccccg gaacgccatc
     1921 cggttccccg cggcctcggc ttcggtctcg cctaccggct ctggttccgt ttcctcaccg
     1981 agccttcgca acacttccct gcgaaccctg cccggggcga tacccaggtt ggagaagact
     2041 ctggccgcca caccctcacc ttccctcacc aggccaagaa ggatgtgctc ggtgccgatg
     2101 tagttgtggc ccagctgcat cgattctctg agcccgagct ccatcacctt cttcgagcgc
     2161 ggcgtgaacg gtatctgcct gcccgagtct tctccatagc cgatgatgct ctcgacctga
     2221 tctcgcacgg cccccagggt gacgcccagc gaagagagcg actgggccgc aacgcctcta
     2281 ccctcgctca caagccccag caaaatatgc tccgtgccta tgtagttgtg gttgaagtga
     2341 cgggcctcat cctgcgcgag caccatgacc ttgcgggccc gttccgtgaa cctctcgaac
     2401 atgctggtga acctcctgcg cccccttgca aacacactca gtctagcgcc ttcggctccc
     2461 ctctgaccgc cgaagtacgg cttcggcgac cgcgagcgcg tcgcggttgg cgcgcacgtc
     2521 gtgcacgcgg aagtacctgg cgcccttctg gtatgcgagc accgtggtag ccacggtacc
     2581 gaagacgcgc tcactcgcca cttctacgcc cgtgatagtg cctatgaacc tcttgcgcga
     2641 ggcgccgacg atgacaggaa aacccagatc tacgatggcg tccaggtcgc gcagtagcgc
     2701 caggttgtgc tcaaggctct tcccgaagcc gatgccggga tcgacgataa tgttctcggg
     2761 cctgacaccc gcagcgacgg catactcggc cctctgcgcg aggaagtccc tgacctctcg
     2821 caccacgtca tcgtagaagg gttcctcctg catggtcttc ggctcgcctt tcatgtgcat
     2881 caggacgacg gggcaccctg cctcggccac gaaggaggcc atgcgaggat cgccccgcag
     2941 cgcggttacg tcgttcacaa cactggcacc ggctccgagt gccgccgccg cggtctcggc
     3001 gcgataagtg tcgatggaga tcacggcctc cggcctgacg gccagtagtc tctcgatcac
     3061 ggggatgacg cgccggatct cctcatcctg cgctacgggg tcggaaccgg ggcgggtcga
     3121 ctctccgccg acgtctatga tcccggctcc ttcgtccagc atggccgctg cgtggttggc
     3181 tgctggttcg ggatctaaga attccccgcc gtcggagaag gagtcggggg tgacgttcaa
     3241 gataccgacg aggacgggac caggtagaaa ggtcccgtcc cctggggtgt tccgggccgc
     3301 aggcgtgcgg ctcatctagt ccaggtagcg gttccggtgg tgtccgtcgg agtcggggag
     3361 agcgccgttc ggcgagacgc cgtccacggc gtactcctcg atcagacgct tcaagtgctt
     3421 ggcgtcgacg gtctcgtact cgatcagctc cgccgagagc ttgttcaaga gttcacggtt
     3481 gcgaatgagg aggtcttccg cggtgtcgta tgactcgtcg acgatcctgc ggatctcctt
     3541 gtctatctgg aaggcgatct catccgagta gtcaggctgg ttgttgaagt ctcgtcccat
     3601 gaagacctgt ccctgctggt gcccaagagc catcgggccg agcttctcgc tcatcccgaa
     3661 gcgggtgacc atctgccgtg ccgtctgcgt cacgcgctcg aggtcgttgg acgctccggt
     3721 ggtgatctcc tcgaagacca cccgctctgc ggccctgccg ccgagcatca tcgcgagctg
     3781 ggccatgagc tgaccgcggc tcatcatgaa gcggtcctct gtggggaggc tcatggtgat
     3841 gccgagcgcc tgcccgcgtg ggattatggt gatcttgtgt acgggatcgg cctcggggag
     3901 gagcgcgccg acgatagcgt gacccgcctc gtggtacgcg gtgatctcct tctccttctc
     3961 ggagatgagg cgcgtcttgc gctcgggacc ggcgatcaca cggtccacgg cctcctccat
     4021 ctcggccatg tcgatctggt ccttgtcatg acgcgcagcc aatagcgcag cctcgttgac
     4081 gaggttcgca aggtccgcgc cggtgaaacc cggagtcccc ctggcgagcg tatctatggt
     4141 gatgtcgggg cccaagggct tgccgcgggt gtggacgcgc aggatcttct ccctgccggg
     4201 aaggtcgggc ctgtcgacca cgatctgcct gtcgaaacgg ccaggccgca ggagcgcggg
     4261 gtcgaggatg tcggcgcggt tggtcgcggc gagcatgatg atcccgctct tcgaatcgaa
     4321 accgtccatc tcgacgagga gctggttgag ggtctgctcg cgctcgtcgt gacc
//
LOCUS       DATAIR010000162         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_235311, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000162 DATAIR010000000
VERSION     DATAIR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235311"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VHM69_03910"
     CDS             <1..1057
                     /locus_tag="VHM69_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX2739567.1"
                     /translation="FERKVVLVCGTAYAGEIKKSIFTVLNFVLPTNCDVFPMHCSANA
                     GQNGDVALFFGLSGTGKTTLSADSERGLIGDDEHGWSDSGVFNFEGGCYAKTIDLSRE
                     KEPQIWEAIRFGAVLENVGMDRRTREVDYSDRLLTENTRVAYPLEYITGAVPEGRGGH
                     PSAVLFLTADAFGVLPPIGALSPEQAAYYFLSGYTAKLAGTEAEMETDVEATFSTCFG
                     APFLPLPATTYAEMLSERLREHGVRCYLINTGWSGGPYGVGSRIDIVATRQMVRAVMG
                     GRLDDAETRKDPFFGLNVPVEVPGVDSSVLDPRGTWDDGDAYDEQARKLADLFRENFQ
                     RFESQVSEEVRNAGPHS"
     gene            1136..>1782
                     /locus_tag="VHM69_03915"
     CDS             1136..>1782
                     /locus_tag="VHM69_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2739568.1"
                     /translation="MADKPYLFANDVPGRREEVIALAREAEARGFPGLFTESPGDNVA
                     LSLALLDGTERITVGTGIAGIYLRHSHTMATGASLIEELHPGRFLLGVGVSHIPFHEE
                     MGISPGKPLADLSQYVESIRSVTDGMKCPPIIVAALRKKMTALAGQIADGVMWANAAL
                     SHVPFSLAQIAPERRAAMIISNSAPACVSEDRRAALEAIRRYLLFYVKLPNYQNY"
BASE COUNT          336 a          561 c          566 g          319 t
ORIGIN      
        1 cttcgagcgc aaggtggtcc tcgtctgcgg cactgcctac gcgggggaga tcaagaagag
       61 catcttcacc gtgcttaact tcgtgctgcc gaccaactgc gacgtcttcc cgatgcactg
      121 ctcggccaac gcgggacaga atggggacgt cgccctcttc ttcgggctct cgggcacggg
      181 caagacaacc ctctccgccg actccgaacg cggcctgatc ggggacgacg agcacggatg
      241 gtcggatagc ggggtcttca acttcgaggg cggctgctac gcgaagacta ttgacctctc
      301 ccgtgagaag gaaccccaga tctgggaagc catccgcttc ggagcggtgc tcgagaacgt
      361 cgggatggac cgccgcacgc gcgaggttga ttactcggac cgcctcctga cggagaacac
      421 ccgcgtcgcc tacccgctag agtacataac aggagccgtc cctgagggcc ggggtggcca
      481 tccttccgcg gttctcttcc tgaccgccga cgcctttggg gtgttgccgc caataggtgc
      541 cctctctccg gagcaggcgg cctactactt cctctcaggc tacacggcga agctcgcagg
      601 gaccgaagct gagatggaga ctgatgtcga ggcgaccttc tccacgtgct tcggtgcccc
      661 gttccttccg ctgcccgcca cgacctacgc agagatgctc tcggagaggc tgcgggagca
      721 cggcgtgcgg tgctacctga tcaacaccgg ctggtcgggc ggtccatatg gtgtcggtag
      781 ccgcatagat atcgtcgcca cccgccagat ggtccgcgcc gtgatggggg gcaggctgga
      841 cgatgccgag accagaaagg atcccttctt cggcctcaac gtacccgtcg aggtgcctgg
      901 agtcgacagc agcgtcctcg acccacgcgg gacctgggac gatggcgacg cctacgacga
      961 acaggccaga aaactcgccg acctgttccg cgagaacttc cagaggttcg agtcgcaggt
     1021 ctcggaagaa gtgaggaacg cgggaccgca ctcgtagagg catcagcaag taaggtctgc
     1081 cgcacttggg cacacccttt cgtcagacgc gccctggtat ctgctggagg acgtgatggc
     1141 cgataaaccg tacttgttcg caaacgatgt ccccggacgc cgtgaagagg tgatcgcgct
     1201 cgcccgcgag gcggaggcgc gcggttttcc tggcctgttc actgaaagtc caggggataa
     1261 cgtggcgctc tcgctggcgc tcctcgacgg gacggagcgg atcacggtcg ggaccggcat
     1321 agccggcatc tacctgcgcc attcccacac gatggcgacc ggggcgtcat tgatcgagga
     1381 gctccacccc gggcgctttc tgctgggtgt cggcgtctcg cacataccgt tccacgagga
     1441 gatgggcata agccccggaa agcccctcgc cgacttgagc cagtacgtcg agagcatacg
     1501 ttcggtgaca gatgggatga agtgtccgcc gataatagtc gccgcacttc gcaagaagat
     1561 gacggccctg gccgggcaga tcgctgacgg ggtaatgtgg gcgaacgcgg cactctccca
     1621 cgtgccattt tcgctggccc agatagctcc cgagcgccgc gcggcgatga taattagcaa
     1681 ctcggctccc gcctgcgttt cggaggatcg cagggcggcg ctagaggcga tacgaaggta
     1741 cctgctcttc tacgtaaagc tgcccaacta ccagaattac tt
//
LOCUS       DATAIR010000163         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_9903, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000163 DATAIR010000000
VERSION     DATAIR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9903"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VHM69_03920"
     CDS             complement(<1..507)
                     /locus_tag="VHM69_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2739569.1"
                     /translation="MVEVSFLASFVGGLLSLLSPCSALLLPAFFAYAFQSRTELVGRT
                     AIFYVGLCATLVPLGMGLSAVSALVYGHRSTLITVSGLVIIAFGVIQLLGGGFAFGPI
                     ERLRGGIKGHSLAATFVLGAVYGFAGFCSGPILGAVLTVAASSGSVLRGAVLLATYAA
                     GMAAPLFLM"
     gene            complement(500..949)
                     /locus_tag="VHM69_03925"
                     /pseudo
     CDS             complement(500..949)
                     /locus_tag="VHM69_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(962..1276)
                     /locus_tag="VHM69_03930"
     CDS             complement(962..1276)
                     /locus_tag="VHM69_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739570.1"
                     /translation="MPGGIPLIGKLLDGCAQARQGRWTLLILVTLVAAVFAGCGGAGG
                     VGSAQSSGQEGQRASQEAGPSEEKSVAASGEDRATSERLGHPSFGSADAPVVLIEFGD
                     YQ"
     gene            1463..>2353
                     /locus_tag="VHM69_03935"
     CDS             1463..>2353
                     /locus_tag="VHM69_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2739571.1"
                     /translation="MEKQKVLITGGAGFLGINLARHLLSRGYAVSSLDVEDFDYPERD
                     RVEIVKGDIRNKALVDRAVESADFVVHTAAALPLYSPEDIYTTDVEGTRNVLDASLRH
                     GVKRFVHISSTAVYGIPDHHPLYETDKLEGVGPYGQAKIQAEMLCLEYRAKGLNVPII
                     RPKSFIGPERLGVFALLYDWAHTGHNFPMIGSGNNRYQLLDVEDLCDAIALTLTLPEE
                     RVNDTFNIGAREFTTMKEDYQAVLDYAGHGKKVVGFPAAPAIWGLRVLDKLKLSPLYK
                     WVYETASKDSFVSIEKAERQL"
BASE COUNT          483 a          778 c          701 g          391 t
ORIGIN      
        1 catcaggaag agaggagcgg ccataccggc tgcgtaggtg gcgagtagta cggcgccccg
       61 aaggactgag cccgaagacg ctgcgacggt gaggaccgcg ccgaggatgg gtccggagca
      121 gaagcctgcg aaaccgtaga cggcaccgag aacgaacgta gcggcgaggg aatggccttt
      181 gatcccaccc cgcagacgct cgataggtcc aaacgcgaac ccgccgccca ggagttgaat
      241 aacgccgaag gcgattatga cgaggcccga gacggtgatg agggtgctcc tgtgcccgta
      301 gacgagcgcg ctgacggcgg agaggcccat cccgagcggc acgagcgtcg cacagagacc
      361 gacgtagaat atggccgtcc tcccgacgag ctcggtacgc gactggaagg cgtaggcgaa
      421 gaacgcgggc aggagaagcg cgctgcacgg gctgaggagg gagagcaggc ccccgacaaa
      481 ggaggccaga aacgatacct caaccacccc cagcctcccg acgggcatcc tcgatggcgt
      541 gctcgaagac atccaggggc tggaagccaa ccagcgcctt tccgttgatg acgaaggtcg
      601 gggttccgct gattcccagg ctctggccct cctggaagtc cgcctgcacc accccggcgt
      661 aacgcgcggt tctgaggtct tcctcgaacc tctgtgcgtc gagacccgcc ttcgtagcga
      721 gcgcgatgag gttctcctcg ctgtagccgc cgctgttacc tgaggattgg ttgtagtaga
      781 gcaggtcgtg gtactcccag aacttgcctt gcgcctgggc tgagcgcgcc gcaagggccg
      841 cgcgcaccga ctcctctccc tgataaggga agtctcgcca ttcgatcctg agcgtcccgt
      901 ccttcacgta cttctcgatg agctccggtt ccacctcacg ggtgaacttg ccgcagtacg
      961 gtcactgata gtccccgaac tctatcagca ccaccggcgc gtcagccgac ccgaacgaag
     1021 ggtgcccgag ccgctcactc gtggcccgat cctccccgga cgccgccacc gacttttctt
     1081 cagaaggtcc ggcctcttgc gaagcccttt gcccctcctg cccggaagat tgcgcggatc
     1141 ctacgccacc cgcgccgccg caaccggcga agaccgccgc aacgagcgtg accaggatga
     1201 gaagagtcca cctcccttgc cgcgcctgcg cgcatccgtc cagcaatttt ccgatcagcg
     1261 gtatccctcc tggcaagcct cagatcttca gcaactgtta taaaccatac gtcgccagag
     1321 cgtaaacgga ctggtaatca ggcacttcgt cgggagcaaa gctgccacac ggcctctttc
     1381 agggagatga gtgctaaaat tcgccgcttg cgcggacctc tatgcgtacg ccagttcgat
     1441 acacacaagg agattcgatt tcatggaaaa gcagaaagtc ctaatcaccg gcggggccgg
     1501 gttcctgggg atcaacctcg caagacacct cctctcgcgg ggctacgccg tttcctccct
     1561 cgatgtcgaa gacttcgact atccggagcg cgaccgcgtc gagatcgtca agggcgacat
     1621 ccgcaacaag gccctggtgg acagggctgt agagagcgcc gacttcgtcg tccacactgc
     1681 ggcggccctg ccactctatt cgcccgagga catctacacg accgacgtgg aggggacccg
     1741 caacgtcctc gatgcttcac tgcggcacgg agtaaagcgc ttcgtccaca tctcttcgac
     1801 ggcggtctac gggatacccg accaccatcc cctctacgag accgacaagc tggagggcgt
     1861 cgggccttac gggcaggcca agatccaggc cgagatgctc tgcctggagt accgcgcgaa
     1921 ggggctgaac gtcccgatca tccgcccgaa gtcgtttatt gggcctgagc gtctcggcgt
     1981 cttcgcgctc ctctacgact gggcgcacac ggggcacaac ttccccatga tcggctcggg
     2041 caataaccgc taccagttgc tcgacgtcga ggacctgtgc gacgcgatag ccctgaccct
     2101 gaccctgccg gaagaacggg tcaacgacac cttcaacatc ggagcccgcg agttcaccac
     2161 gatgaaggaa gactatcagg ccgtcctcga ctacgccggg cacggcaaga aagtcgtcgg
     2221 cttccccgcc gcaccggcga tctggggcct gcgcgttcta gacaaactga aactctcccc
     2281 cctttacaag tgggtctacg agacggcgtc gaaggactcc ttcgtctcca tagagaaggc
     2341 cgagaggcag ctc
//
LOCUS       DATAIR010000164         7499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_327533, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000164 DATAIR010000000
VERSION     DATAIR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327533"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHM69_03940"
     CDS             complement(<1..692)
                     /locus_tag="VHM69_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2739572.1"
                     /translation="MTRYEPADSFETPRFSGVRTFMRLPNTQDLENADVAIVGAPFDT
                     GATFRAGARFGPEGIRGASHLLRRYNPSLDVAIFDHLSVIDYGDVPVVPGYIEESYRR
                     IEEGLGLIHRAGVVPIVLGGDHSIALPELRAAAAVHGPLALVQFDSHPDTWDAYFGEK
                     HTHGTPFRRAVEEGLLDTSRSIQVGMRGSIYDHNDWDDAREMGFDLVPTDEVRELGIP
                     RMIDRIRERAGDA"
     gene            complement(732..2156)
                     /locus_tag="VHM69_03945"
     CDS             complement(732..2156)
                     /locus_tag="VHM69_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2739573.1"
                     /translation="MAEAGAEKRDRGRQEGHLQQGLKRRHMTMIAIGGVIGAGLFVGT
                     GPILNAAGPATILTYLLTGCILILIMRMLGEMAVAQPSVGSFSDYSRMALGNWAGFAV
                     GWLYWYFWAIVVGFEATVAASILGEYIPGVAPWLIALLLILLLTATNLYSLGSYGEFE
                     FWFAGIKVAAIILFIVLAALFVIGILPGGGIGLTNLYAKGGFFPNGGLVMFSGVVTVI
                     FAFVGAEIVTIAASESNEPEQGVARATNQVIYRILLFYVVSVFFLAAVVPWNTTFAEE
                     VIKSPFTIAFEKMGIPFAPTLMNFVVLTAVLSVLNSSLYTTSRMLFALTRHNDAPGFF
                     TDTTRRGVPMWAILAGTSFGYVSVVIYYFFPENVFTWLINASGAIALFVYLLIAISEL
                     VMRRRLERDAPERLQLKMWLYPWLTYLSIAAMVAVLIAMFVIPDQRPLLIASLAALVV
                     ILVAYLLRRQFGPPEKSPEEVAER"
     gene            complement(2250..3404)
                     /locus_tag="VHM69_03950"
     CDS             complement(2250..3404)
                     /locus_tag="VHM69_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2739574.1"
                     /translation="MSIHFSADELAGRRMAAVEELAERGLDGLLMFRQESMYYLTGYD
                     TFGYVFFQCLYMGADGETLFLLTRTPDVRQAGHTSVIEDIRVWYDVEDADPARDLKEI
                     LDEHGCRGRRLGIETDAYGLTGYNLKRVEAALDGFCELVEASDLVTRLRSVKSPAEIE
                     YVREAARLADLGLDRAVEVAGPGVFEGEVLAALQGAIFEGGGDFPANEVIIGSGPRAL
                     LFRSAAGMRRMNPVDQLSLEWAGSYRHYHAAMMRTLAVGEASEYQRHLFSAVREALEE
                     MTEALTPGRPVGEVDDAHRRVLDAAGLGEHRFAACGYSLGTTFSPNWMDWPMLFSGNS
                     VQAEPGMVFFLHCVVADSERGVAMSLGRTCLVTDAGREVLSARPLELIVV"
     gene            complement(3445..4671)
                     /locus_tag="VHM69_03955"
     CDS             complement(3445..4671)
                     /locus_tag="VHM69_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2739575.1"
                     /translation="MKASTVIVGGGIWGLSTAYHLARAGWAKVQVLERNGTLFDETTS
                     QAAGLVGQIRATPLMRRAIRYAIDLFLDFERETGHDPGFRQAGSLLLALTPERMASFE
                     EHVEHANSGGVEARFVDGTEMSELAPHLDMRRVEGGYFVPEDGYLDPRQCARAFCAAA
                     EDLGVRILPGTAVTGLTIGDGRVIGVETDRGFVEAEHVVVTAGPWTGTVAKMAGHRTA
                     LVPIRHQRVTTASVSGIPDHHPVVRVTDASCYLRPEEGGYLYGIFEPDPTSYDPDLPS
                     GFHTSDIEEPVEVMEEARKRLSPIFPVLKELGIAGYKRGLTTFAPDGAYLVGPVPQVE
                     GLFAATGCAALGIAGSAAVGRWLADWIVRGDPGEDLSSIGLDRFGDKACDNDWIRRES
                     EEFYGGYYSIPAMSVT"
     gene            complement(4709..6088)
                     /locus_tag="VHM69_03960"
     CDS             complement(4709..6088)
                     /locus_tag="VHM69_03960"
                     /inference="COORDINATES: protein motif:HMM:NF005812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2739576.1"
                     /translation="METRKDPRARELAEKDDRYVWHALSRYTPTEGGGAAPAPRMIVA
                     EGEGAWISDTEGNRYLDGMSGLWCVNVGYGREELAKAAYAQLTKLPYYPLTMSHEPAV
                     ELGEKLNEWLDDDYIVYYSNSGSEANEVAFKVARQYQRQNGEPGRHKFVSRYRAYHGN
                     TFGSLAATGQAQRKHRYEPLASGFLHVPPPDRYRCAYCADRSACNLECAREIGRTIAW
                     ELEETVAGVVMEPIITGGGIIIPPEGYVEEVAQICERTGTLLIIDEVICGFGRTGKKF
                     GHQHHAVKPDIVTMAKGITSGYLPLSATAVRRKVFEAFKGTEEYDHFRHVNTFGGNPA
                     ACALAIRNLQIMEEENLPGRSAEMGERLWERLRELADHPNVGEIRHRGLLFGIELVED
                     SESRAPARAEKVASVIAGCKERGIIVGKNGDTVAGFNNILTLAPPLIVSDDDLEFISS
                     ALKESVRSL"
     gene            complement(6102..7220)
                     /gene="ald"
                     /locus_tag="VHM69_03965"
     CDS             complement(6102..7220)
                     /gene="ald"
                     /locus_tag="VHM69_03965"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2739577.1"
                     /translation="MATVIGVPKEIKDMEGRVSMQPDGVAELVHHGHEVVVQAGAGAG
                     AGFTDEEYAEAGARVVEGPDEVFADSELIVKVKEPVPEEYDRFKEGQQLFTYLHLAAD
                     KRLTEFLMERKINSIAYETVEADDGSLPLLTPMSEVAGRMAIQAAAHHLESPQGGAGL
                     LLGGVPGTPAAKVTIIGGGVVGTEAAKIAVGMRAIVRILDVDPKRLAYLSDIFEGRVD
                     LVIPNRARTAFYVAESDVVVGAVLVHGAKAPKLVTREMVASMRPGSLVVDVAIDQGGC
                     IETARPTTHSEPTYVEEGVVHYCVANIPGAVARTSTLALTSATLPYLIKIADKGIEGA
                     AEDPALAKGLSTLRGDLVSEPVAEAHGLSFTDAGKLLS"
BASE COUNT         1360 a         2458 c         2325 g         1356 t
ORIGIN      
        1 gcgtcgccgg cccgctcgcg gatgcggtct atcatcctcg gtatcccgag ttcgcggact
       61 tcgtccgtcg ggactaggtc gaagcccatc tcgcgcgcgt catcccaatc attgtggtcg
      121 tagatcgaac cccgcatccc gacctggatg gaacgcgagg tgtccaaaag tccctcttcg
      181 acggcacgcc ggaacggcgt gccgtgcgtg tgcttctcac cgaagtacgc gtcccaggtg
      241 tcagggtgcg aatcgaactg cacgagcgcg agcggtccgt gtacggccgc cgctgccctg
      301 agttccggta gcgcaatgga atggtcgccg ccaaggacta tgggaactac tccagctcgg
      361 tggatcagcc cgagaccctc ctctatgcgc cggtagctct cctcgatgta gccggggacg
      421 accggcacgt ctccatagtc gatgaccgag aggtggtcga agatcgccac gtccaggctg
      481 gggttgtagc gcctgaggag gtgagaggcg ccgcgtatcc cctcagggcc gaagcgggcc
      541 ccggccctga aggttgcgcc cgtgtcgaac ggcgcgccga ctatcgcgac gtccgcgttc
      601 tccaggtcct gcgtgttagg caggcgcatg aaggtgcgga cgccggagaa gcgcggcgtc
      661 tcgaaagagt cggcaggttc gtagcgggtc aagagactat ccctctttac tcaggatgcg
      721 cggggcgtcc cctacctctc ggccacttcc tccggagatt tctccggggg tccgaactgg
      781 cggcgaagca agtaagctac caggatcacg acgagagcag ccaagctcgc tatcagcagg
      841 ggccgctgat cggggatcac gaacatcgcg atgaggacag caaccatggc ggctatcgac
      901 aggtacgtaa gccatggata gagccacatc tttagctgga gcctctcggg cgcatcacgc
      961 tccaggcggc ggcgcatcac gagctccgag atggcgataa gcaggtagac gaagagcgcg
     1021 atggcacccg aggcgttgat cagccacgtg aatacgttct cggggaagaa gtagtagatc
     1081 accacggaga cgtagccgaa gctggtcccc gcgaggatgg cccacatcgg cacgccgcgg
     1141 cgggtggtgt cggtgaagaa ccctggcgcg tcgttgtgcc tcgtcagggc gaagagcatc
     1201 cgggaagtcg tgtacagact ggagttgagg acggagagca cggcggtcag taccacgaag
     1261 ttcatcagcg tgggcgcgaa gggtatcccc atcttctcga acgcgatggt gaacgggctc
     1321 ttgatgacct cttcggcgaa cgtggtgttc cacggaacga ctgcagcgag gaagaagacc
     1381 gagacgacgt agaagagcag gatcctgtag atgacctggt ttgtcgcgcg ggccacgccc
     1441 tgctcgggct cgttggactc ggacgccgca atggtgacga tctcagcccc gacgaacgcg
     1501 aagatcacgg tgacgacacc ggaaaacatc accagaccac cgttggggaa gaagccgccc
     1561 ttggcgtata ggttcgtgag gccgatgcca ccgccaggca aaatgccgat cacgaacagc
     1621 gcggccagga cgatgaacag gatgatcgcg gcgaccttta tacctgcgaa ccagaactcg
     1681 aactcaccgt aggacccgag agagtagagg ttggttgcgg tgagcagcag gatcaggagc
     1741 aaagctatga gccagggcgc tacacccggt atgtactccc ccaggatgct cgccgccacg
     1801 gtggcctcga agccgaccac gatcgcccag aagtaccagt agagccaccc gacggcaaaa
     1861 ccggcccagt taccgagagc catccggcta tagtcggaga aggagccgac cgaaggctgg
     1921 gcaaccgcca tctccccgag catccgcatg atcaggatca ggatgcaccc cgtcaacaaa
     1981 taggtgagga tggtcgccgg accggccgca ttgagaatcg gtcccgtgcc cacgaagaga
     2041 ccggccccga tgaccccgcc gatggcgatc atggtcatgt gtcgtctctt gagaccctgt
     2101 tgcaggtgac cttcctggcg gcccctgtcc cgcttctctg cgcccgcttc agccatcttt
     2161 ccccttccct atgtaccttc ccttcccgtg catcttacag cttatgcacg ctcttttaca
     2221 gcttcagagg gagcttctcc gatccaactt cagacgacga tcaactcaag tggccgcgcg
     2281 gagagaacct cccttccggc gtccgtgacg aggcaggtgc ggcccaggct catggcgact
     2341 ccgcgctcgg aatcagccac cacgcaatgg aggaagaaga ccatccctgg ctcggcctgc
     2401 acggagttcc ccgagaacag catcggccag tccatccagt tggggctgaa ggtcgtgcct
     2461 aaagagtatc cgcacgcggc gaagcgatgc tccccgagcc ccgccgcgtc gagcacacgg
     2521 cggtgggcgt cgtcgacctc gccgacagga cgtccgggag tgagcgcctc ggtcatctct
     2581 tcgagagcct cgcggacggc tgagaaaagg tggcgctgat actcagaagc ctctcccacc
     2641 gccagggtac gcatcatggc ggcgtggtag tgacggtagg aaccggccca ctccagggac
     2701 aactggtcta cggggttcat gcgacgcatg cctgcggcgg agcggaacag gagggcgcgg
     2761 gggcctgagc cgatgatcac ctcgttcgcg gggaagtccc cgcctccctc gaagatggcg
     2821 ccctgcaaag ccgcgaggac ctccccctcg aagacgccgg gccctgctac ctctacggcc
     2881 cggtcgaggc cgaggtcagc gagccgtgct gcctcccgca cgtactcgat ctccgcaggt
     2941 gacttgacgg accgcaatcg ggtcacgaga tccgaagcct ccaccagctc gcagaatcca
     3001 tcgagcgcag cctcaacgcg tttcaggttg tagcccgtga ggccgtaggc gtcggtctct
     3061 atccccagac gcctgccacg acacccgtgc tcatcgagga tctccttcag gtcccgggcg
     3121 ggatccgcgt cctctacgtc gtaccagacc cggatgtcct cgatcaccga cgtgtgcccg
     3181 gcctggcgca cgtcgggagt tcgggtcagg aggaagaggg tctcgccgtc agcccccatg
     3241 tagaggcact ggaagaagac gtagccgaag gtatcgtacc ctgtgaggta gtacatcgac
     3301 tcctggcgga acatgagcag accgtcgagg ccgcgctccg ccagctcttc gacagcggcc
     3361 atgcgacggc ccgccaactc atcggctgag aagtgtatgg acacaataat cggctcctcg
     3421 gacagggata gctctgatcg gcgcctaggt cacggacatg gcggggatac tatagtagcc
     3481 gccgtaaaac tcctcgctct cgcgccttat ccagtcgttg tcgcaagcct tgtccccgaa
     3541 gcggtcgaga ccgatcgaag acaggtcctc cccagggtca ccccgtacga tccagtctgc
     3601 cagccagcgg cccaccgcag cggagcccgc gataccgagg gccgcgcacc cggttgcggc
     3661 gaagaggccc tccacctgcg gcacagggcc gaccaggtag gcgccgtcag gggcgaaggt
     3721 ggtcaggccc cgcttgtagc cggcgatccc aagctccttc aacacgggga agatcggaga
     3781 cagcctcttg cgggcctcct ccatgacctc cacgggctcc tcgatatccg acgtgtggaa
     3841 ccctgacggt agatccggat cgtagctcgt cggatcaggt tcgaagatcc cgtataagta
     3901 tccgccctct tccggcctca gatagcaact cgcgtcggta acgcggacga cagggtgatg
     3961 gtccggtata cccgagacgg aagccgtagt gacacgctgg tggcgtatgg gcaccagcgc
     4021 cgtccggtga cccgccatct tcgctacggt ccccgtccag ggtccggccg tcaccacgac
     4081 gtgttcagcc tcgacgaaac cccggtccgt ctcgacccct atcacccgcc cgtcacctat
     4141 ggtaagcccg gtcaccgcgg tgccgggcag tatcctcacg ccaaggtcct ccgcggcggc
     4201 gcagaaggcc cgagcgcatt gccgggggtc gaggtagccg tcttcgggga cgaagtaccc
     4261 gccctcgacg cgcctcatat ccaggtgtgg cgccagttcg gacatctccg tcccgtccac
     4321 gaagcgggcc tccacgccgc cgctgttcgc gtgctcgacg tgctcttcga aggaggccat
     4381 ccgttcgggc gtaagggcaa gcaaaaggct tccggcctgg cggaatccgg gatcgtgtcc
     4441 ggtctcccgc tcgaagtcca ggaacaagtc tatggcgtac cttatcgccc gcctcattag
     4501 aggggtggcc cggatctggc caaccaatcc ggcggcctgg gaggtcgtct catcgaacag
     4561 agtgccgttg cgctcaagaa cctgcacctt cgcccatccg gctcgcgcca gatggtaggc
     4621 ggtactcaaa ccccagatac cgcccccaac gatcacggta ctagctttca ttgctatctc
     4681 ccactatgtc agactatgtg ggagcggttc atagactacg cacgctttcc tttagcgcgc
     4741 tggagatgaa ctccaagtca tcatcgctta cgatcagcgg aggagcgagg gtcaggatgt
     4801 tgttgaaccc cgccaccgta tcgccgttct tgccgactat gatacctcgc tccttgcaac
     4861 cggcgatgac cgatgccacc ttttccgccc tggcgggcgc gcggctctca ctatcctcca
     4921 cgagttcgat gccgaagagt aggcccctgt gccgaatctc tccgacgttc ggatggtctg
     4981 cgagctcgcg aagccgctcc caaagtcgct cgcccatctc cgccgaacgt cctggcagat
     5041 tctcctcttc catgatctgg aggttcctta tagccagagc gcatgccgcc gggttaccgc
     5101 cgaaggtgtt gacgtgacgg aagtggtcgt actcttccgt gcccttgaag gcctcgaaga
     5161 ccttacgcct gaccgccgtg gccgagagcg gcaggtagcc gctggttatg cccttggcca
     5221 tcgtcacgat atccggcttt acggcgtggt gctggtggcc gaacttcttg cccgtccttc
     5281 cgaaaccgca gatcacctcg tcgatgatca gcagggtccc ggtgcgttcg cagatctgtg
     5341 cgacctcttc gacgtagccc tcgggcggga tgatgatccc acctccggta atgatgggct
     5401 ccatgacgac ccctgccaca gtctcctcaa gctcccacgc gatggtgcgc cctatctccc
     5461 tcgcgcactc caggttgcag gcggagcggt cggcgcagta ggcgcagcgg tatcggtcgg
     5521 gcggcgggac gtgcaggaag ccggacgcga gcggctcgta gcggtgcttg cgctgggcct
     5581 gcccggtcgc ggcgagcgag ccgaaggtgt tgccgtggta ggcccggtag cggctgacga
     5641 acttgtgcct gccaggctcc ccgttttgcc tttggtactg gcgggccacc ttgaaggcga
     5701 cctcgttggc ctcggagccg gagttggaat agtagacgat atagtcgtca tccagccact
     5761 cgttcagctt ttcgccgagt tctacggcgg gttcgtggct catggtgaga gggtagtaag
     5821 ggagcttcgt gagctgcgcg taggcggcct tcgccaactc ctccctaccg tagccgacgt
     5881 tgacgcacca caggccggac atgccgtcga ggtagcggtt gccttcggtg tcggagatcc
     5941 aggctccctc gccctctgcg acgatcatcc tcggcgccgg ggctgcgccg cctccctcgg
     6001 tcggcgtgta gcgggagagg gcgtgccaga cgtagcggtc gtccttctcg gccaactcac
     6061 gggccctcgg gtcctttctt gtctccactg cggttacctc ctcagctcag gagcttgccg
     6121 gcgtcagtga aggagaggcc atgggcctcg gcgaccggct cggagacgag atctcccctc
     6181 agcgtagaca atcctttggc cagcgccggg tcttctgccg cgccctctat acctttgtcc
     6241 gcaatcttga tcaggtaggg caacgtcgcg ctggtcaggg cgagcgtcga ggtcctcgcc
     6301 acggccccgg gtatgttggc cacgcagtag tgcacgacac cctcttccac gtacgtcggc
     6361 tcggagtgcg tggtcggcct cgcagtctct atgcacccac cctggtctat ggcgacgtcg
     6421 actaccaggc tacccggtcg catgctcgcg accatctcac gcgtgacgag cttgggcgct
     6481 ttcgccccgt gcaccagcac ggccccgaca acaacgtccg actcggcgac gtagaaggcg
     6541 gtccttgcgc ggttggggat caccaggtcc accctgccct cgaagatgtc cgagaggtag
     6601 gcgaggcgct tggggtccac gtcgaggata cgcacgatgg cccgcatgcc gaccgctatc
     6661 ttggccgcct ccgtcccgac gacgccgccg ccgatgatcg tgaccttcgc cgcaggagtc
     6721 cctggcacgc ccccgagcag gagcccggcc ccgccctgcg ggctctcgag gtggtgcgcc
     6781 gccgcctgaa tcgccatcct ccccgccacc tcgctcatcg gcgtgagcag cggcagagac
     6841 ccgtcatcag cctcgaccgt ctcgtatgcg atggagttga tcttccgctc catcaagaac
     6901 tccgtgagcc tcttgtccgc cgccagatgc aggtaggtga agagctgctg accctccttg
     6961 aagcggtcgt actcctcggg gacgggctcc ttgaccttca cgatcaactc agaatccgcg
     7021 aatacctcgt cgggtccttc gacgacgcgc gccccggcct cggcgtactc ctcgtcggtg
     7081 aagccagcgc cagcgccggc cccggcctga acgaccacct cgtgcccgtg gtgtaccagc
     7141 tcggcaacgc cgtcaggctg catggagacg cgcccctcca tgtccttgat ctccttcggt
     7201 acccctatca ctgtcgccat ggatcttcct ttccctcgtt gcccccacgc ccttcttata
     7261 gcaaggttac tatagtcgtg cacacatgct ggcaaggggc ccgtggtgct tgcttttgtg
     7321 gtgggaacat tacaataatg tttcaagcgt acagggaacg gcattgggag gagcgttggc
     7381 gaagggaagc ggttcgatgg ggttctccat aggcgaggtc tcgcaggcgg taggtctagc
     7441 gccgcagacc ctgcggttat gggagaggga gcgcctgatc acaccccgca ggacggacc
//
LOCUS       DATAIR010000165         5261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_50679, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000165 DATAIR010000000
VERSION     DATAIR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50679"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..363
                     /locus_tag="VHM69_03970"
     CDS             103..363
                     /locus_tag="VHM69_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX2739578.1"
                     /translation="MAEREMVIGAEEGLHARPAAQFVNKAKQYSSDIVVVKGGREANA
                     KSPLKIMTLGARKGDKVTIRAEGDDAEEAVEVLAQLVSADGQ"
     gene            376..1995
                     /gene="ptsP"
                     /locus_tag="VHM69_03975"
     CDS             376..1995
                     /gene="ptsP"
                     /locus_tag="VHM69_03975"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX2739579.1"
                     /translation="MKLTGIGASEGVTVGLAFVPATGKLEPERQRIAECEIESELQRF
                     QQAVEAVAAKLTETGERLRATGNEEEAGIFDAHVELAEDPELGSGVEERVRNQESPEA
                     AVLAVGEEYAAELADLDDEYMSARADDVRDVAKQIAAELMGGGASAFETLETPSVVLA
                     RSLAPSETARIPKGMALGFVIAEGSRTSHVSIMARSMGIPAVVGVGPALEEALDAKMV
                     AVDGGEGYAITDPDADTLAEFERMSEAMAEERAALDEYRHVEARTSDGRRIEVAANLG
                     SASEAEDALERGAEGVGLFRTEFLFMERDDLPSEDEQYEAYSAVARVFGKKPVIIRTL
                     DVGGDKDLPGVDQHHEQNPFLGWRGIRMSLDVPGLFKPQLRAILRAAVHGNLKVMFPM
                     VVDVEELKAAKKMLEESSAELEVEGKEIGEVETGVMVETPAAALCAEELAKEAAFFSI
                     GTNDLVQYTLAADRGNERLRRLQSADHPAVLELIRQTCEAASEAGIPVGVCGEAAGEP
                     EMIPKLVDLGVTELSMGAPSIPRAKKVVSEL"
     gene            1992..2732
                     /gene="nagB"
                     /locus_tag="VHM69_03980"
     CDS             1992..2732
                     /gene="nagB"
                     /locus_tag="VHM69_03980"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX2739580.1"
                     /translation="MIAHVFPDYDAMSAAVADAVAQRLREDPSTVFLLPTGTTPLGMY
                     RRLVEMHRSGEISFSRATFFNLDEYLGLPPDHPASYHVYMKENFYGHIDADPARIFVP
                     NGAAPDPEAECERYEAAITEAGGIDVCVLGIGRNGHIGFNEPGAPFDSRTRVVRLSES
                     TRLVNAGDFESDRAPECAITVGMATIFESKEVLLLASGTNKAAAVAAAVEGEVTEEVP
                     ASMLKIHPNATLFLDEDAASDLGQGVAG"
     gene            2947..3942
                     /locus_tag="VHM69_03985"
     CDS             2947..3942
                     /locus_tag="VHM69_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2739581.1"
                     /translation="MKVGLLGAGRIGALHADVLARDEDVDTILVGDADPERAESVAGE
                     VGGEAGKIEDVLASEPDAVVIAASTTAHADLIRASVEAGIPAFCEKPIALGYDETVEI
                     VDLVESSGATLQIGFQRRFDAGYVEARRLVESGALGTLYSIRLATHDPEPPPEEYIPA
                     SGGIFRDLHIHDFDILRWLTGGEVEEVFATGSVRKFDFFADHGDVDTSAALITMEDGV
                     VAVLTGGRHDPLGYDVRAEVFGSGDSVVVGLDRRTPLRSVEPGMPPSEKPAYPNFQER
                     FLDAYRAEMGHFLSLVRGEAENPCTARDALEALRIAMAADLSLAEHRPVLLSEVG"
     gene            3939..4823
                     /locus_tag="VHM69_03990"
     CDS             3939..4823
                     /locus_tag="VHM69_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2739582.1"
                     /translation="MKAKPRIAGAPISWGVCEAPGWGFQMEAERVLREAAGLGLTAVE
                     AGPDGFLPPDPEKMSGLLSGYGLELVGGFVPAVLHRPEARDPELASVERQARLFAAAG
                     ASVLVLAADTDQEGYEESAGLSDNEWEELFETLAMVEEIGARHGLSVVLHPHYGTEIE
                     SPEQVQRFLEGCDTALCLDTGHSMVGGGDPVELAESAAGRIRHVHLKDVDQSLTERVV
                     AGTLDYEKAVRDGLYRPLGDGDVDVRRVLEVMDGAGFDGWYVLEQDIMLEAEPDDGPA
                     DEVGRSLTFLKGVLEVEV"
     gene            4820..>5261
                     /locus_tag="VHM69_03995"
     CDS             4820..>5261
                     /locus_tag="VHM69_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2739583.1"
                     /translation="MNPEVVTMGRVSVDLYPEQIGVPLAEVKTFAKSLGGSSTNVAVA
                     AARLGHPSAVITKVGDDGFGPYIRTALEDFGVDAAWVGTDPDLRTPLAFCEIHPPDDF
                     PLLFYREPKAPDMNLTVEDLDLEAIAAAPLFWMTGTGLSDEPSRT"
BASE COUNT          977 a         1558 c         1873 g          853 t
ORIGIN      
        1 agtaagaaac aggagcggcc ctgtcgtgcc ggaacttcat cccggttcgg gtggggtatg
       61 atgggtgccg cgtcctggat cttatggtga gaggagcgac tcgtggctga gagggagatg
      121 gttatagggg ccgaggaagg actgcacgcc aggccggctg cgcagttcgt caacaaggcg
      181 aagcagtatt catcggatat cgtggtggtc aagggcgggc gtgaggcaaa cgcgaagagt
      241 ccgctcaaga tcatgaccct cggggccagg aagggagaca aggtcaccat aagggcagag
      301 ggcgacgacg ccgaggaggc cgtcgaagtc ctggcccagc tcgtctccgc cgacgggcag
      361 tagtcgagcc catacgtgaa gcttaccggc ataggcgcat cggaaggggt gacggttggt
      421 ctggcgttcg tgccagccac gggcaagctg gagccagagc ggcagcgaat agcggagtgt
      481 gagatcgagt ccgagctgca acggttccaa caggcggtcg aggcagttgc agccaaactt
      541 acggagacgg gggagcgtct ccgcgcaacg ggtaacgagg aggaggccgg aatcttcgac
      601 gcccacgtcg agctcgctga ggaccccgag ctgggatcgg gtgtcgagga gcgcgtgagg
      661 aaccaggaga gccccgaggc cgctgttctc gccgtcggcg aggagtacgc tgccgagctt
      721 gcagacctgg acgacgagta catgtcagcc cgcgcagacg acgtgcgcga tgtggcgaag
      781 caaatagcgg ccgagctgat gggtgggggc gcctcagcct tcgagactct ggagacgccc
      841 tcggtcgtgc tcgcgcggag cctcgcgcct tcggagacgg cgcgcatccc caaggggatg
      901 gcactcgggt tcgtcatcgc agaggggtcg aggacttcgc acgtctcgat catggcccgc
      961 tccatgggca tccccgctgt agtgggtgtg ggaccagccc tggaagaggc gctggatgcg
     1021 aagatggtcg ctgtggatgg cggggagggc tacgccataa ctgaccctga cgcggatacg
     1081 ctcgccgagt tcgagaggat gtcggaggcg atggccgagg agcgggcagc cctcgacgag
     1141 taccgtcacg tggaggcccg caccagcgac ggacggcgca tcgaggtcgc tgcgaacctt
     1201 ggctctgcgt cggaggcgga agacgcgctg gagcgggggg cggaaggcgt cgggctcttc
     1261 aggacggagt tcctgttcat ggagcgcgac gacctgcctt ccgaggacga gcagtacgaa
     1321 gcatacagcg ccgtcgcgcg ggtgttcggt aagaaacctg tcatcatccg tacgctcgac
     1381 gtgggaggag acaaggattt gccaggtgtg gatcagcatc acgaacagaa tcccttcctc
     1441 ggctggcggg gtataaggat gagcctggac gtcccagggc tgttcaagcc tcaactgcgc
     1501 gcgatactgc gcgcggcggt ccacggcaac ctcaaggtca tgttcccgat ggtagtcgac
     1561 gttgaggagt tgaaggccgc gaagaagatg ctggaggaga gcagtgcgga gctcgaagtg
     1621 gaaggaaagg agatcgggga ggtcgagacg ggcgtgatgg tagagacacc ggcggccgca
     1681 ctctgcgccg aggagcttgc gaaggaagcc gcgttcttct ctatagggac gaacgacctc
     1741 gtgcagtaca cgctcgccgc ggacaggggc aacgagcgac tgcgccggct gcagagtgca
     1801 gaccatcccg cggttctgga gttgatccgg cagacttgcg aggctgcgag cgaagcgggc
     1861 atccccgtag gcgtgtgcgg cgaggcggcc ggcgaacccg agatgatccc gaagctagtg
     1921 gatctaggcg tcacggagtt gagcatgggc gcaccatcca taccgcgggc caagaaggtc
     1981 gtctccgagc tatgatagcg cacgtctttc ccgactatga tgccatgagc gcggccgtcg
     2041 ccgatgccgt cgcccagagg ctcagggaag acccttcgac ggtcttcctc ctgccgacgg
     2101 ggacgactcc cttggggatg tacaggaggc tcgtcgagat gcaccgctcg ggcgagatct
     2161 ctttctccag ggccaccttc ttcaacctcg acgagtatct ggggctgccg cctgaccatc
     2221 cggcgagcta ccacgtgtac atgaaggaga acttctacgg gcacatcgac gcagatccgg
     2281 ccagaatctt cgtcccgaac ggggcagcgc ctgacccaga ggcggagtgc gagcgctacg
     2341 aggcggccat aacggaggcg ggtggtatcg acgtgtgcgt gctcggcatc ggacgcaacg
     2401 gtcacatagg cttcaacgag ccgggcgccc ccttcgactc caggacccgc gtcgtgaggc
     2461 tctccgaatc cacccgtctg gtcaacgcgg gcgacttcga gtccgaccgc gcgcctgagt
     2521 gtgcgatcac ggtcggcatg gcgaccatct tcgagtcaaa ggaggtcctg ctcctcgcct
     2581 ccggcacgaa caaggccgct gccgtggcgg cggccgtgga gggtgaagtc acggaggagg
     2641 tgccagcatc gatgctgaag attcacccga acgccacgtt gttcctcgac gaagacgccg
     2701 cctcggacct gggacagggc gtcgcgggct agcggatgcc ggcggttggt ccgacccccg
     2761 ttgacgcggg ttggtcgctc aagtagcatc gttgtgggtt gaaatggggg ctagagggaa
     2821 ggttgatttc gtcgcgcact ggacaggttt gtccctcgtg tgccctctaa tacgatggga
     2881 gaggaaccga gatgagagac aagcaattct gctcgccgcc gggcgtcgag gagtgagggg
     2941 gtccgaatga aggtcggact gcttggagct ggccgcatcg gggccctcca cgccgatgtg
     3001 ctcgcgcgcg acgaggacgt ggataccata ctcgtggggg acgcagaccc agagcgcgcc
     3061 gagtcggtcg ccggggaggt cggaggcgag gcagggaaga tcgaggacgt gctcgcttcc
     3121 gagccggacg ccgtcgtcat cgccgcctct accacggcgc acgcggattt gatccgggcc
     3181 tccgtcgaag ccggtatccc ggccttctgt gagaagccta tcgcactggg ctacgatgaa
     3241 accgtcgaga tagttgacct ggtcgagtca tccggtgcca ccctccagat cggcttccag
     3301 agacgcttcg acgcgggata cgtcgaggcg aggcgcctgg tcgagagcgg tgcccttggt
     3361 accctatact cgatccggct cgccacccac gaccctgagc cgccgcctga agaatacata
     3421 ccggcctccg ggggcatctt ccgcgacctc cacatacacg acttcgatat cctgcgctgg
     3481 ctgacagggg gcgaggtcga ggaggtgttc gccacgggca gcgtgcgcaa gttcgacttc
     3541 ttcgccgatc acggcgacgt ggatacctct gcggccttga tcacgatgga agacggcgtg
     3601 gtggccgtcc tgacgggcgg gcggcacgac ccacttggct acgacgtgcg ggccgaggtc
     3661 ttcggctctg gcgacagcgt cgtcgtcggc ctggaccggc gcacgcccct gcgttccgtc
     3721 gagcccggga tgccgccttc cgagaaaccc gcctacccga acttccagga gcgcttcctt
     3781 gacgcctaca gggcggagat ggggcacttc ctgtctttgg ttcgcgggga ggccgaaaat
     3841 ccgtgtacgg cccgcgacgc gctggaggcg ctcaggatcg cgatggccgc agacctctcg
     3901 ctcgcagaac accgcccagt cttactatcg gaggtcggat gaaggcaaag ccacgcatag
     3961 ccggagcgcc catctcctgg ggcgtgtgcg aagcgccggg atggggcttc cagatggagg
     4021 cggaaagggt gctgcgcgag gccgcgggcc tgggcctgac tgcggtagag gccgggcccg
     4081 acggttttct gccgcccgac cccgagaaga tgtccgggtt gctctcaggg tatgggctgg
     4141 agctggtcgg gggcttcgtt cccgccgtcc tccaccgtcc cgaggcgcgc gacccggagc
     4201 tcgcctcggt cgagcggcag gccaggctgt tcgcggcagc cggagcaagc gtgctcgtgc
     4261 tcgccgcgga taccgaccag gaaggatacg aggagtcggc agggctgagc gacaacgagt
     4321 gggaggagtt gttcgagacg ctcgcgatgg tggaggagat cggggccagg catggcctga
     4381 gcgtcgtgct ccatccccac tacgggaccg agatcgaaag ccccgagcag gtacagcggt
     4441 tcctggaagg ctgcgatacc gcgctgtgcc tggacacggg acattcgatg gtcggtgggg
     4501 gggatcccgt cgagctggcc gaatccgcgg cagggaggat ccgtcacgtc cacctaaagg
     4561 acgtggatca gagcctcacg gagcgggtcg tcgccggcac tctcgactac gagaaagccg
     4621 tcagagacgg cctctacagg ccgctcggag acggagacgt ggacgtcagg cgtgtgctcg
     4681 aggtaatgga tggggccggc tttgacggat ggtacgtgct tgaacaggac atcatgctgg
     4741 aggcggaacc agacgatgga cccgcggatg aggtaggcag gagcctcacc ttcctgaaag
     4801 gcgtgctgga ggtagaagta tgaatcccga ggtcgtgacg atggggcggg tgagcgtgga
     4861 cctttatcca gagcagatcg gggtccccct cgcggaggtc aagacgttcg ctaaatccct
     4921 cggcgggagc tccacgaacg tggccgtcgc agcggcccgc ctcggccacc cctcggcggt
     4981 aataacgaag gtcggcgacg acggcttcgg cccatacatc cgcaccgcgc tcgaagactt
     5041 cggggtggac gccgcatggg tcggaacgga cccggacctt cggaccccac tcgctttctg
     5101 tgagatccac cctcccgacg acttcccgct gctgttctac cgcgagccca aagccccgga
     5161 catgaacctg accgtcgagg acctcgacct cgaagccatc gccgccgcgc ctctgttctg
     5221 gatgacgggt actggcctct ccgacgagcc gagccgcacg g
//
LOCUS       DATAIR010000166         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_10318, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000166 DATAIR010000000
VERSION     DATAIR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10318"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>2616
                     /locus_tag="VHM69_04000"
     CDS             49..>2616
                     /locus_tag="VHM69_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2739584.1"
                     /translation="MTIPTNYEPIPQPPGYPVLGNLFDLRGADTAVQALMKLASQYGP
                     IFQLRVGPTTMIVVSGFDLADELCDDTRFDKMLGRGQINARAVVGDGLFTSWTHEPNW
                     KKAHNILLPNFGMRAIRNYFPMMTDIAMQLVQKWERLNPEQDIDVPADMTRLTLDTIG
                     LCGFDYRFNSFYSEEPHPFVASMIRCLDLSMRRQTRLPIGERLRVREHRQLQEDTTYM
                     NSLVDEIISERKASGGESETKDLLNSMLTGVDKETGEHLDDVNIRYQIITFLIAGHET
                     TSGLLSFALYYLLNNPEVLAKAYEEVDRVLGDDTSSPPTNVQVNHLRYVSQVLKETLR
                     LWPTAPGFTRYPYEPTVLGGKYQIDKQNSLLVLTPMLHRDPKIWGDDPDAFDPDRFTP
                     EAEQERPANAYKPFGTGQRACIGRQFAMLEATLVLGMILQRFELVDHTNYGLKIKQTL
                     TIKPADFHIKVRARTNRKAAPLAPAPQPAIVQEELEAEPVTMVQGNTPLLVLYGSNLG
                     TTEGIAHQIADDATTRGFTTTIAPLDDYVDKLPKEGAVVIASSSYNGTPPDNAAGFFD
                     WLCNDSIGEGALKDVKYTVFGCGDRDWAATYQRVPKMIDSQMAAYGAERIYQRGEADA
                     SDDFDGQFRGWYDHLWSSLADALGTSVEAMDDVPQGHRYEVEFVTGPVEVSPLVAEYD
                     ARPMEVLANRELQNKSGPHPSERSTRHIELAVPEGISYKAGDHLGILPRNSEELVGRV
                     LGRFDLADGALIRIRNNTQSKTFLPVDEPVSASALLSSYVDLQGVARRSEIAVLAEYT
                     ECPPEKERLLALGGDDPDSVELYRKEVLEKNVSLIDLLEEFMACEVPFNVYLELVPLL
                     "
BASE COUNT          575 a          809 c          756 g          476 t
ORIGIN      
        1 atttggacag acccaaatag gtgctgctga ccaggaggag gccggatctt gaccatccca
       61 accaattacg aaccaatccc gcaaccgcct ggatatccgg tgcttggcaa cctgttcgac
      121 ctgagagggg ctgataccgc cgttcaagcc ctgatgaagc tcgcgagcca gtacgggccg
      181 atcttccagt tacgcgtggg tccgaccaca atgatcgtgg tatcgggctt cgatctcgcc
      241 gacgagttat gcgacgacac gcgtttcgac aagatgttgg gacgaggtca gatcaacgcc
      301 cgcgcagtgg taggcgacgg gttgttcaca tcctggactc acgaacccaa ctggaagaag
      361 gcccacaaca tactccttcc caacttcggt atgcgggcga tcaggaacta cttcccgatg
      421 atgacggaca tcgctatgca gctcgtccag aaatgggaga ggctgaatcc ggaacaagac
      481 atcgacgtgc ccgcggacat gacccgtctc acgctcgaca ccataggctt gtgcggtttc
      541 gactaccgct tcaactcttt ctacagcgag gagcctcacc cgttcgtcgc gagcatgatc
      601 cgttgtctgg atctgtccat gcggcggcag acgcgcctgc ctatagggga gaggttgagg
      661 gtgcgcgagc atcgccagct ccaggaagac accacgtaca tgaactcgct cgttgacgag
      721 atcatctccg agcgcaaagc aagcggaggc gagtctgaga cgaaggactt gcttaactcc
      781 atgctcacgg gggtggacaa ggagaccggc gagcacctgg acgacgtcaa catccgctat
      841 cagatcatca ccttcctcat agccggccac gagaccacga gcggactgct ctccttcgcg
      901 ctgtactact tgctcaacaa ccccgaagtg ctcgccaaag cctacgagga ggtagatcgg
      961 gtactcggag acgacacttc gtcgccgccg accaatgtcc aggtgaacca tctgaggtac
     1021 gtctcccaag tgctcaagga gactctgcgg ctctggccaa cggcgcctgg ctttaccagg
     1081 tatccatacg agccgacggt ccttggcggc aagtaccaga tagacaagca gaactcgctg
     1141 ttggtgttga cgccgatgct ccaccgcgac cccaagatat ggggcgatga tccggatgcg
     1201 ttcgatcctg acaggttcac tcccgaggcc gaacaggaga ggccagccaa cgcctacaag
     1261 ccctttggca ccggacagcg ggcgtgcatc gggcgccagt tcgcgatgct cgaggccacg
     1321 ctggtgctag ggatgatcct gcagcgtttc gaactcgtcg accacacgaa ctacgggctg
     1381 aagataaagc agaccctcac catcaagcca gccgacttcc acatcaaggt gcgggcgcgc
     1441 acaaaccgca aagcggcacc cctggccccc gctccgcagc cggcaatagt tcaggaggaa
     1501 ctagaagccg aaccggttac gatggtgcag ggcaacaccc cgctgctggt cctctacggc
     1561 tccaacctgg gcaccacaga aggcatcgcc caccagatcg ccgacgacgc gacgacccgc
     1621 ggtttcacca ccaccatcgc ccccctcgac gactacgtgg acaagctacc gaaggaaggg
     1681 gccgtggtaa tagcctcctc ctcttacaat ggtacccccc ctgacaacgc cgctggtttc
     1741 ttcgactggc tgtgcaacga ctctatcggc gagggtgctc tcaaggacgt gaagtacacc
     1801 gtcttcggct gcggcgaccg tgattgggct gcgacgtacc agagggtgcc caaaatgatc
     1861 gacagccaga tggcggccta cggggcagag cgcatctacc agcgtggtga ggccgacgcg
     1921 agcgacgact tcgatgggca gttccgcggt tggtacgatc acctgtggag ctctcttgcg
     1981 gatgcgctcg gcacctctgt tgaagcgatg gatgacgtac cacaggggca tcgctacgag
     2041 gttgaattcg tcaccgggcc cgtcgaggtc agtccgctcg ttgctgaata cgacgcaagg
     2101 ccgatggaag tactcgccaa cagggagttg cagaacaaga gtggtcctca tccttccgag
     2161 cgctcaaccc gccacatcga gctggcagtt cccgaaggaa ttagctacaa agcgggcgac
     2221 cacctcggga tattgccgcg taacagcgag gagctggtgg gccgcgttct agggcgcttc
     2281 gaccttgctg atggcgcact cattcgcatc cgcaacaaca cccagagcaa gacgttcctg
     2341 cctgtcgacg agccggtctc cgcctcggcg ctgctttcaa gctacgtgga cctccaaggc
     2401 gttgcaaggc gctctgagat agccgttctt gccgaataca ccgagtgccc cccggagaaa
     2461 gagcgactgc tcgcactcgg tggcgatgat cccgacagcg tggagctcta cagaaaggag
     2521 gtccttgaga agaacgtctc cctgatagac ctgcttgagg agttcatggc ttgcgaggtg
     2581 ccttttaacg tctacctcga gctagtgccg cttctg
//
LOCUS       DATAIR010000167         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_379465, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000167 DATAIR010000000
VERSION     DATAIR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379465"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHM69_04005"
     CDS             <1..275
                     /locus_tag="VHM69_04005"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2739585.1"
                     /translation="DFVEVFVDAPIEVCAERDVKGLYKKAYAGEIEQFTGVSDPYEPP
                     NAPDLVLKTDEELPQESARKVIEKLEFFGYLWPREAQDTEYNWSSR"
     gene            304..1050
                     /locus_tag="VHM69_04010"
     CDS             304..1050
                     /locus_tag="VHM69_04010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2739586.1"
                     /translation="MNAAQLQDRVAVVTGASSGIGAAVARALNAGGARVALAARREDA
                     LIEVREGFAGHGKSIIIPTDVTDREQVRSLVARAEEELGPVEILVNCAGVMYYTLMKN
                     LREEEWERTVEVNCKGAINCVGAVLPGMLERGRGHIVTISSDAGRKVFPGLAVYSASK
                     FFVEALSQGLRLETAGTGLKVTTVQPGNVATDLLSMSVDKEALEMYGQPGGARLLEPE
                     DVAASVVHALVQPEHVAVNEILVEPRDEPV"
     gene            1071..1535
                     /locus_tag="VHM69_04015"
     CDS             1071..1535
                     /locus_tag="VHM69_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2739587.1"
                     /translation="MNKFPTHILLATDGSPHADLAARMAIDLAHSTGSRLHVVAVGRT
                     FPPAALYDEYAESRSEDLRREAQEILDEQVRKIEEAGGTVAIAHLKMGEQRDEAIVHL
                     AEDIDAGLIVIGSRGFGGMKRALLGNVADSVVRHAHCPVLVVRPTEGASRPF"
     gene            complement(1542..>1911)
                     /locus_tag="VHM69_04020"
     CDS             complement(1542..>1911)
                     /locus_tag="VHM69_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739588.1"
                     /translation="AASLTMTRIRDDVSEQRLKVEAALALGATSRQAVAPILKTALQS
                     ALIPLIDATKTTGIIFLPGAMVGMIIAGADPLEAVRLQIVVLYMLLGSVSIAAILVGT
                     LSYRSFFTPRHQLRTAQNQS"
BASE COUNT          361 a          564 c          674 g          312 t
ORIGIN      
        1 tggacttcgt cgaggtattc gtcgatgccc caatcgaggt ttgcgccgag cgggacgtga
       61 agggcctgta caagaaggct tacgctggtg agatagagca gttcacgggc gtctcggacc
      121 cttacgagcc gccgaacgcc cccgacctcg tcctcaagac cgacgaggag ttgccacagg
      181 agagcgcgag gaaggtcatc gaaaagctcg agttcttcgg gtacctgtgg ccgcgagagg
      241 cgcaagacac ggagtacaac tggagctccc gctgagggtc agtcccgacc ggaattcttc
      301 cctttgaacg ccgctcagct ccaggaccgg gtcgccgtgg tgaccggagc ttcgagcggc
      361 atcggcgccg ccgtagcccg cgctctgaac gcagggggag cgcgcgtcgc gcttgcggcc
      421 cggcgcgagg acgcgctcat cgaagtcagg gaaggtttcg cgggccatgg gaagagcatc
      481 atcatcccca ccgacgttac ggaccgcgag caggtccgtt cgctggtggc gcgtgcggag
      541 gaggaactcg ggcccgtgga gatcctcgtc aactgcgcgg gggtcatgta ctacaccctg
      601 atgaagaacc tgcgcgagga ggagtgggag cggaccgtcg aggtcaactg caagggcgcc
      661 atcaattgcg tcggggcggt gctgccgggg atgctggaga gaggacgggg gcacatcgtc
      721 accatctcct ccgacgcagg acggaaagtc ttccccggtc tcgcggtcta ctcggcgagc
      781 aaattcttcg tggaggcgct ttcgcagggc ttgcggctgg agaccgcagg cacgggcttg
      841 aaagtaacga ccgtacagcc aggcaacgtg gcaaccgacc tcctctcgat gagcgtggat
      901 aaggaggcac tggagatgta cgggcaaccg ggtggagcca ggctgctgga acctgaggac
      961 gttgcggcgt cggtggtgca cgcgctcgtg cagcccgagc acgtcgccgt aaacgagatc
     1021 ctggtcgagc cccgcgatga accggtctga ggacctcgaa aggaggaaga ttgaataaat
     1081 ttccaacgca catcctcctg gccaccgacg gctctccgca cgcggatctg gcggccagga
     1141 tggcgataga cctcgcccat agcaccggct cgcgcctgca cgtcgtcgcg gtcggacgta
     1201 cgttcccacc cgccgccctc tatgacgaat acgccgaatc acggagcgag gatctccggc
     1261 gcgaggccca ggagatcctc gacgaacagg taaggaagat cgaggaggca gggggtaccg
     1321 tcgcgattgc ccacctgaag atgggcgaac agcgggacga ggcgatcgtc cacctcgccg
     1381 aggacatcga cgcgggcctc atagtgatcg gcagccgcgg gttcggaggg atgaaacgcg
     1441 ccctcttggg caacgtcgcc gactcagtcg tccgtcacgc acactgcccc gtcctcgtcg
     1501 tgcgcccgac cgagggtgcc agccggcctt tctgaagcac ttcagctctg gttctgcgcc
     1561 gttcgtagct ggtggcgtgg ggtgaagaag gagcggtacg agagcgtacc gaccaggatg
     1621 gcggcgatgg agacgcttcc gaggagcatg tagaggacga ctatctggag gcgtacggct
     1681 tcgagcgggt cggcgccggc gatgatcatg cctaccatcg cgccagggag gaagatgatc
     1741 cccgtggtct tcgtggcgtc tatgagggga atgagggcgc tttgaagcgc ggtcttgagg
     1801 attggcgcga cggcctggcg gctcgtggcc ccgagtgcga gggcggcctc gaccttgaga
     1861 cgttgctcgg atacgtcgtc gcggatacgg gtcatcgtca ggctcgcggc g
//
LOCUS       DATAIR010000168         9414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_235520, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000168 DATAIR010000000
VERSION     DATAIR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235520"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..824)
                     /locus_tag="VHM69_04025"
     CDS             complement(126..824)
                     /locus_tag="VHM69_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2739589.1"
                     /translation="MARKKMKGAKIGVKVGRRVAPVAGRAAGKVAWKAGKGQARLVRR
                     AMSSQEPKKSKYLKYGFFALLGLAVGAAIARSKSGDTSSESPSYTDTTGNHAPDPGSP
                     AGRRGETWGSGTPTGTAGGGGLQSPEEQNRTGAEREYSDPSSGPLIGEQHGGSVEGVG
                     EQQEEVEQRIRTSIGEDSRTAELPRVNVEVNDGIAELRGEAPSEDAKLAAGEIAQQVE
                     GVREVRNLITVSTE"
     gene            complement(993..1595)
                     /locus_tag="VHM69_04030"
     CDS             complement(993..1595)
                     /locus_tag="VHM69_04030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2739590.1"
                     /translation="MQGSLPDIQVPAQLVLYSIPCVIYAIVQRLRGRAWSHIASNLGL
                     RGSRPVYYGWALVFFVVLGLISWIMIPVFFPGYFDSPGIAASQYAGVELGILTVLYAF
                     FRESVYVAFGEELFFRGLVGGWLMRHLGFWVGNALQSLVFLVPHLFVLSGGLNLWPLL
                     LAPLISGWLNGWLLYRSGSILPAWLGHSLGVAFAAAGAMS"
     gene            complement(1963..2781)
                     /locus_tag="VHM69_04035"
     CDS             complement(1963..2781)
                     /locus_tag="VHM69_04035"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HEX2739591.1"
                     /translation="MSETLSGRQNVAVDSREAFAAWIDSAPDGRIFGQVFVRRVSPSG
                     YSLRHSADAETDDLEVREDPRSAREIAKLTENGEYRPLKSSPNLRRGWEIRVSDTPGL
                     ATAMNYLYPAGVVHWYLHREGRLDVTSFRENAARQSGIYKRVQRLSDGGVHDAVRACC
                     EDAVCLKKTLWDVDESTPLDIDRGEGEIPCPEPCSVFVSFARRVRLFEREAKRDLDAI
                     GLSPSEREDLAALVEAAAKGEVRFAREAEFEKPLNERRMRYRRLTLVPKLRQDG"
     gene            complement(2774..3529)
                     /locus_tag="VHM69_04040"
     CDS             complement(2774..3529)
                     /locus_tag="VHM69_04040"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX2739592.1"
                     /translation="MRALVIVGHGSHLNEDSSLPVYEHAGRIRETGEFDEVVECFWKE
                     EPSMRHVLDTVESEVVYVVPAFISEGYFTQQVIPRELGLEGPVTRKGDKTVHYAGPLG
                     TFEGMVDVILERTDDLMRGKEVSGRTALVLLGHGTDLNKNSGGVIYLNAGRIRERGIY
                     DLVDVGFLDQDPEIGGVVDSVEAENAILIPVFIAEGWHTRETIPEDLGLTGEVTVREG
                     KTIYYGAPVGTHPSMANLIAARARETMAEEHVV"
     gene            complement(3600..4487)
                     /locus_tag="VHM69_04045"
     CDS             complement(3600..4487)
                     /locus_tag="VHM69_04045"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX2739593.1"
                     /translation="MERPSTQFVPEGMVAACVATALTLVYSAPTPGTGSRYIDGRVAH
                     EARVLSVVPVRDALAVSGRRGVGETVLEATLSSLSLSGFADPRGAGYLAPLARAAVRG
                     EPSARVLAGLGHDEIAPFARALDATRGEGALAGSLSYALGAGKDATLRDAMRFSASRD
                     ALAREYARDFEVTRELARPALLSALSRSDSVRGALVQAYLEVLSEVPDLDVAGRSGRH
                     EAEDVSRMARGVLKAGGVHSRRGLEGIANLAGLLRADPRLSPTTTEYPVIAAAFLVAV
                     EYGPDALSHRLRPATGVDR"
     gene            complement(4494..4631)
                     /locus_tag="VHM69_04050"
     CDS             complement(4494..4631)
                     /locus_tag="VHM69_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739594.1"
                     /translation="MKGARTRREICLAPGARGLREGHSTFESSDREDVGAVPGDLGGG
                     R"
     gene            4718..5851
                     /locus_tag="VHM69_04055"
     CDS             4718..5851
                     /locus_tag="VHM69_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX2739595.1"
                     /translation="MHTRDDISLIPKDAFEKGIREGTLGAEEELWLADPETFKLADGA
                     QKILALEPEGQFSGELIDCEIESNTGVHEQPTGVVADLVSRRRVLLGHADRLGRVLGT
                     SGTHPIGDWREQEIINKPHYQRLKKKLGWLIRRNNTFSVHVHYAVHGRGKAVYLFNRL
                     REYIPHMLALSVNSPFWQGEYTDMHSARVLMFSRAYHRAGLPGPLGSWREYADYVDYL
                     KRSGLIDALGEIWWDVRLHPGLDTVEIRAFDAQTDPARTEALVALAAALCDMLVLEHE
                     SGEPRPIHPNREIEENKWSAQRYGLEGVFIDHVTHEAVDTRAAIEDLVSLLSATTERD
                     LSGLRRVLDEPTESARQLEVWRETGSTVEVTKDLAARTRASAL"
     gene            complement(5857..5988)
                     /locus_tag="VHM69_04060"
     CDS             complement(5857..5988)
                     /locus_tag="VHM69_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739596.1"
                     /translation="MKKAIAIRLLKSKKARQMAVKVLKNKKVRKIVVNQAKRRVVGN"
     gene            complement(6013..7047)
                     /locus_tag="VHM69_04065"
     CDS             complement(6013..7047)
                     /locus_tag="VHM69_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739597.1"
                     /translation="MGGHVLFETGIRRLGSKENGFFYRYPGTGETVREERVLARIENL
                     KVPPAWEDARIAHSPSAKVQAVGYDSAGRLQYLYNARYREKKELEKFERVLRFADALP
                     EMRKATSDHLRRKELGYEKVLAAMTRLMNAAYFRVGEERYAKHNGTYGIATLRRKHLR
                     IQGDTMIFEYGGKWGKTQRKVVTDRRLREVVEECVELPGYEVFKYLDENAEVRDVKSR
                     DLNRYVKEVMGDEFTPKDFRTWAGTMIAAVKLAELGPATGSKDAEKNVLEAVDAVAER
                     LGNTRDIARSSYISPRVIDHYLEGSVVAYHGEHLEEVIAAEQGNLTEGEKALLELLNK
                     KLRREIRKAA"
     gene            complement(7156..7989)
                     /locus_tag="VHM69_04070"
     CDS             complement(7156..7989)
                     /locus_tag="VHM69_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739598.1"
                     /translation="MSDRPEESREDRRRTGPLGDDDDETRRVPPGENDPEATRQVPKA
                     RSGEEGGASAGTRQVPQRATEPEDKETRVIRTQGYADTSGEATPYPRGYFEAAEEREA
                     RLRDMYGGVDWLASFLGFVFAVVLGAVFSGIAGLVLVPFGFPPDLGTGRIGASVITGL
                     VLLGVLIFLTYFFGGYVAGRLARFDGGRNGAMVLAWNFIVVLILALAALVFSGFLPSV
                     AAESVGNIVDGAVSNVQNLAGAGAVGFVAALAAILIALLGGFLGGRMGSRYHTEIDRA
                     A"
     gene            8280..>9414
                     /locus_tag="VHM69_04075"
     CDS             8280..>9414
                     /locus_tag="VHM69_04075"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2739599.1"
                     /translation="MDTADRQNVLQQAQENNVQFIRLWFTDVLGTLKSFAITVEELED
                     ALDGGMGFDGSSITGYQDIEESDMIAMPDPSTFQVIPWSPQDAPTARMICDVRTPDGD
                     PYVGDPRHVLRRALERANEMGFDKFYCGPELEFFYFKDSKGTEPLDYGGYFDLTTLDA
                     ATALRRETVQSLQQLGIKVEYSHHEVGISQHEIDLRFDDAMTMADKVMTYKTVVKEVA
                     TRHGVYATFMPKPLRDQNGSGMHTHQSLFSGGKNAFFDPDNEYYLSSEAQAFIAGQLR
                     HAREISIIFAQHVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGQERATRMELR
                     CPDPAANIYLCFAALLHAGLEGIEKGYELPEPMERNLYHLTSEE"
BASE COUNT         1669 a         3156 c         2834 g         1755 t
ORIGIN      
        1 ctgagagcat ttcagcctgt cagcacgctc gggccttcgg ccctctcatt cagcgagaac
       61 gccccgccaa gacggcgggg cgtctgtaca accggtgggt gccacactct gcgtatgctg
      121 actagctatt ctgtgctgac agtaatcagg ttacgcacct cgcgcacgcc ttcgacttgc
      181 tgggcgatct caccggccgc gagcttggca tcctcggagg gcgcctcccc gcgcagctcg
      241 gcgataccgt cgttgacctc gacgttcacc cgcggcagct ccgctgtgcg cgagtcctcg
      301 ccgatgctgg tccggatgcg ttgttcgacc tcctcttgct gctcgcctac gccctcgacg
      361 ctaccgccat gctgctcgcc gatcaggggt cctgacgagg gatccgagta ctctcgctcg
      421 gcaccggtgc ggttctgctc ctccgggctc tggaggccac cgccgcccgc ggtgcctgtc
      481 ggggtaccgc tcccccaggt ctcgccccgc cggccagccg ggctccccgg gtcgggggca
      541 tgatttccgg tcgtgtccgt gtaggagggg gactcactcg aggtatcacc gctcttggaa
      601 cgcgcgatcg ctgccccgac ggcgagcccg agcagggcga agaagccata cttgagatat
      661 ttggacttct tcggctcctg agagctcatc gcccggcgaa cgagcctggc ctggcccttg
      721 cccgccttcc acgcgacctt ccctgcggcc cttccggcca cgggcgctac ccttcttccg
      781 actttcacgc caattttggc gcctttcatc ttcttcctcg ccatgcacta cctccatctt
      841 cgcggagtag gtttcgaaag aacgagatac cccgaaacgc ctctcgggaa acagggcgca
      901 ttacctgcgt ccccctcagc ctgttcgtca cgccttctac gcatagcacc gccaggacga
      961 ctgccgcggt ggccctctcc cagacgcggc ccttaactca tcgcgcctgc ggcggcgaaa
     1021 gcaacgccca ggctgtgccc caaccatgcc ggcaggatgc tccccgaacg gtacaacagc
     1081 cagccgttga gccagccgga gatcaacggt gccaacagca gaggccagag gttcagacca
     1141 ccgctcagca cgaacaggtg gggcaccagg aaaacgaggc tctgcaaagc gttgcctacc
     1201 cagaatccca ggtgcctcat caaccagccg ccgaccagcc cacggaagaa gagttcctct
     1261 ccgaatgcta catagacgga ctctcggaag aacgcataga ggacggtgag tatgcccagt
     1321 tctacgcccg catactggga agcggcgatg ccgggactgt cgaagtaacc tgggaagaat
     1381 acgggaatca tgatccagga aatcaacccc aaaacgacga aaaagacgag tgcccagcca
     1441 tagtagacgg gccggctgcc tcgcagcccg aggttggaag ctatgtgtga ccatgcccta
     1501 cctcgaagcc gctggacgat agcgtagatc acgcaaggaa tagagtaaag tacgagttgt
     1561 gcggggacct gtatgtcagg tagagagcct tgcacggccg aacctccggt ctcgccacaa
     1621 tatcactcgc ctggtattgt gcctggaacg gcccctatca gccagcgacg ttttcctgtt
     1681 caccgatgcc acgagacggg gtcttccgac gttcgccgca tgttcatgcg ccatgctgga
     1741 tggtcatttg tacattggcg tccgatgaga gtcgttctgg cggttccctg gcgatgttac
     1801 aaggccgcaa aaggtcgtgt accgcgctgg tgcatcgggg gtcatctttc cgccaccagg
     1861 atggcggaca attgcaggct cggccgtgcc caggatcaga cgttgttccc aggcaccggc
     1921 tggacgagca tggcgtcatc aaccaggaac tcgaaggctt ctctatccgt cctgacggag
     1981 cttggggacg agggtgagcc tccggtagcg catcctcctc tcatttagcg gcttctcgaa
     2041 ctcggcctcg cgggcgaacc ggacctctcc cttggctgcg gcctcgacga gtgccgcaag
     2101 gtcctctctc tccgagggcg agaggcctat tgcgtccagg tcgcgcttcg cttcccgttc
     2161 gaagagcctc acacgcctgg cgaacgagac aaagacacta catggctcgg ggcaggggat
     2221 ctcgccttcc cctcgatcga tgtcgagcgg ggtgctctcg tccacgtccc aaagcgtctt
     2281 cttcaggcac accgcgtcct cgcagcaggc gcggaccgca tcatgtactc cgccatccga
     2341 gagccgctga actctcttgt agataccgct ctgcctggct gcgttctcac ggaagctcgt
     2401 gacatcgagc ctaccttccc gatgtagata ccagtggacg acaccggccg gatacagata
     2461 attcatggcg gtcgcgagcc cgggcgtatc ggagaccctt atctcccagc cgcgccgcag
     2521 gttcggggag ctcttcaggg gcctgtactc tccgttttcg gtgagcttgg cgatctcacg
     2581 ggccgatctt ggatcttcgc gtacttcgag gtcgtccgtt tctgcgtccg cactgtgacg
     2641 cagcgagtag cctgatggtg agacccggcg cacgaacacc tgcccgaaga tgcggccatc
     2701 gggggcgcta tctatccagg cggcgaaggc ctcgcgcgaa tcaacggcga cgttctgccg
     2761 cccggagagt gtctcagaca acgtgctcct ctgccatcgt ctcgcgggcg cgggcggcta
     2821 tgaggttcgc catcgagggg tgggtcccga ccggggcgcc gtagtagatc gttttgccct
     2881 cgcggacggt aacctcgccc gtgaggccga gatcctccgg tatcgtctcg cgagtatgcc
     2941 agccctcggc gatgaagacg gggatcaaga tcgcgttctc agcctccacg ctgtcgacga
     3001 cgccgcctat ctcgggatcc tggtcgagga agcccacatc gacgaggtcg tagatcccac
     3061 gctccctgat gcgtcctgcg ttcaggtaga tgacaccgcc cgaattcttg ttgaggtcgg
     3121 tgccgtggcc gagcaacacg agggccgttc gtcccgagac ctcctttccg cgcatgaggt
     3181 cgtcggtacg ctccaggatg acgtcgacca tcccctcgaa ggtgccaagg ggcccggcgt
     3241 agtggacggt cttgtcgcct ttgcgcgtaa caggtccttc caggccaagc tccctcggga
     3301 tgacctgctg ggtaaagtag ccttccgaga tgaaagctgg gacgacgtag acgacttcgg
     3361 actcgacggt gtcgaggacg tggcgcatgg agggctcctc tttccagaag cactcgacga
     3421 cctcgtcgaa ctctccagtc tcccttattc gtccggcgtg ttcgtatacg ggcaggctcg
     3481 agtcttcgtt gaggtgagat ccgtgtccaa cgatcacgag agccctcata cgcctcctct
     3541 gaagtttctt gactccagtc cgattctatc aatcgagaag ccaggtcggt cgaacggctt
     3601 cacctgtcga cccccgtggc aggcctgagc ctgtggctca aggcgtcggg gccgtactcg
     3661 acggcgacga ggaaggccgc ggcgatgacg ggatactctg tggtggtcgg agagaggcgg
     3721 gggtcggccc gcaagagccc agcgaggttg gctattcctt ccaggccccg tctagagtgg
     3781 acgccgccgg ccttgagtac gccgcgcgcc atgcgggaga cgtcttcggc ctcgtgcctg
     3841 ccggagcgtc ccgccacatc gaggtccggc acctccgaaa ggacctccag gtaggcctgg
     3901 acgagggcac cgcgcaccga gtctgaacga gagagggccg agaggagcgc gggcctggcg
     3961 agctcgcggg ttacctcgaa gtccctggcg tactcgcggg cgagggcgtc gcgcgacgcc
     4021 gagaagcgca tcgcgtctcg cagcgtcgcg tccttgccag ccccgagggc gtacgaaagc
     4081 gagccggcga gagccccctc gccgcgcgtt gcatcgagcg ccctcgcgaa aggggctatc
     4141 tcgtcgtggc cgaggcctgc aagtacgcgc gccgatggct ccccgcgcac tgccgcgcgg
     4201 gccagcggag caaggtaccc agcgcccctg gggtctgcga agccggacag ggagagcgag
     4261 gagagggtgg cctcgagtac cgtctccccc acaccccgcc ggcccgacac ggcgagcgcg
     4321 tcccttacgg gcacgacgga gagcaccctt gcctcgtggg cgacgcggcc gtcgatgtag
     4381 cgggaccccg tccccggggt cggtgcgctg tagacgaggg tgagggccgt cgcgacgcag
     4441 gccgctacca tcccctcagg cacgaactgc gtgctgggac gctccatccg gctctagcgc
     4501 cccccgccca gatcacctgg cacggcccct acgtcttccc tgtccgagga ctcgaatgta
     4561 ctgtgccctt cgcgcagtcc acgtgcgccc ggggcgaggc agatctcacg acgtgtcctg
     4621 gctcccttca tctgcacctc ctgtcgagct atcaagggca tcattctaac ccgaggccgc
     4681 catctctccc cacgtacggg gtaatatact cgtcggcatg cacacccgtg acgacatctc
     4741 actcattccg aaggacgcct tcgagaaggg cattcgggag ggcacccttg gcgcggagga
     4801 ggagctgtgg ctggcggatc ctgagacctt caaactcgcc gacggggcgc agaaaatcct
     4861 ggctctcgag cctgaaggtc agttctccgg ggagctcata gactgtgaga tcgagtcgaa
     4921 cacgggcgtg cacgagcagc ccaccggggt cgtcgccgac ctcgtttccc gaaggcgtgt
     4981 actgctcgga cacgcggatc gtcttggcag ggtcctcggt accagcggta cgcaccccat
     5041 cggtgactgg cgggaacagg agatcatcaa caagcctcac taccagcgtc tcaagaagaa
     5101 actggggtgg ttgatccggc gcaacaatac gttctccgtg cacgtccact acgccgtgca
     5161 cgggcggggg aaggccgttt acctcttcaa ccgactgcgc gagtacatac cgcacatgct
     5221 cgcgctctct gtcaactcac ccttctggca gggtgagtac accgacatgc actcggcgcg
     5281 agtcctgatg ttctcgcgcg cctatcaccg cgccgggctt ccaggcccac tgggctcctg
     5341 gagggagtac gcagactacg tggattacct caagcgctct ggcctcatag acgcactggg
     5401 tgagatctgg tgggacgttc ggctccatcc cggcctcgac acggtcgaga tccgggcctt
     5461 cgacgcccag accgaccccg cccgcacaga ggccctcgtg gccctcgccg cagccctctg
     5521 cgacatgctc gtgctggagc acgaatccgg cgagccaagg cccatacacc cgaaccgcga
     5581 gatcgaagag aacaagtgga gcgcccagcg ttacggtctc gaaggcgtct tcatcgacca
     5641 cgtgacccac gaggcggtcg atactcgtgc cgccatcgag gatctcgttt cgctcctctc
     5701 agcgacgacc gagagggacc tctctggact tcggcgcgtt ctggacgaac cgacggagtc
     5761 ggcgcgccag ctcgaggtgt ggcgggagac ggggtctacg gtggaggtca cgaaggacct
     5821 ggcggcgagg acgcgggcct cggcgctcta gcatccttag ttgccaacca ccctgcgctt
     5881 cgcctggttg acgacgatct tgcggacttt cttgttcttc agaaccttga cggccatctg
     5941 acgcgccttc ttgctcttca gtagcctgat ggcgattgcc ttcttcatga tatgcctcct
     6001 gtttcgtcta tcttaagcgg ctttcctgat ctcacgtcgc agcttcttgt tcagcagttc
     6061 gaggagcgcc ttctcacctt ctgtcaggtt gccctgttcg gcggctatga cctcctcgag
     6121 gtgctctcca tggtaggcta ccacggaacc ctcgaggtaa tggtcgataa cgcgcgggct
     6181 gatgtaggaa gagcgggcga tatcccgggt gttcccgagc cgctcggcaa cggcgtccac
     6241 agcttccagc acgttcttct cggcgtcctt cgacccagtc gcgggcccga gctctgccag
     6301 cttgactgcg gcgatcatgg tccccgccca ggtccgaaag tccttgggcg tgaactcatc
     6361 gcccatgacc tccttgacgt acctattcag gtccctggac ttgacgtctc gcacctcggc
     6421 gttttcgtcg aggtacttga atacctcgta gcccggcagc tccacgcact cctcgaccac
     6481 ctcgcgcagc cttcggtcag tgacgacctt cctttgggtc ttaccccatt tgccgccgta
     6541 ctcgaagatc atggtgtcgc cctgaatccg gaggtgcttg cgcctgaggg tcgctattcc
     6601 gtaggtcccg ttatgcttcg cgtagcgctc ctcgccgacc cggaagtacg ccgcgttcat
     6661 aaggcgcgtc atcgcggcga ggaccttctc gtagcctagc tccttgcgcc tgaggtgatc
     6721 gctggtcgct ttacgcatct cgggcagcgc gtccgcgaaa cgcaggaccc gctcgaactt
     6781 ctcgagctct ttcttctccc tgtacctggc gttgtacagg tactgcaacc tgcctgctga
     6841 gtcgtagccg acggcctgca ctttggcgga tgggctgtgg gcgatccgcg cgtcttccca
     6901 ggcaggcggc accttcaggt tctcgatgcg cgcgaggacc cgctcctcgc gcacggtctc
     6961 tcccgtgcca ggatagcgat agaagaatcc attctccttc gagccgagac gccttatgcc
     7021 agtctcgaat agtacgtgac cgcccaaaac accattcctc tatgacgaca gcgttatcgt
     7081 agccctttac aagcgcgacc catcctttcc cgcaagggcc cgcatacgaa acctgccgaa
     7141 cgaactacgg cgtcttcagg ccgccctgtc tatctccgtg tgatacctgc tgcccatgcg
     7201 cccccccagg aacccgccga ggagtgctat cagtatcgcg gccagggcag cgacgaagcc
     7261 gacagccccg gcgcccgcga gattctggac gttagacacc gctccgtcga cgatattacc
     7321 gacggactcg gcggccaccg agggcaggaa gccgctgaac accaacgcgg cgagggcgag
     7381 gatcaggacc acgataaagt tccatgcgag aaccatcgcc ccgttgcgcc ctccgtcgaa
     7441 ccgtgcgagg cgcccggcca cgtacccgcc gaagaagtag gtcaggaaga tcaggacccc
     7501 cagcaacacg aggcccgtaa tgacggaggc cccgatccgg cccgtcccaa ggtccggagg
     7561 gaagccgaac gggacgagca cgaggcctgc gatccccgag aagacggccc cgaggacgac
     7621 ggcgaagacg aacccgagaa agctcgcgag ccagtcaacg ccgccgtaca tgtcgcgaag
     7681 cctggcctcg cgctcctcgg cggcctcgaa gtaacctctc ggataggggg tcgcctcccc
     7741 gctcgtgtca gcgtatccct gcgtcctgat gacgcgtgtc tccttgtcct caggctcggt
     7801 agccctctgg ggaacctgcc gggtcccagc cgacgctccc ccctcctccc ctgaccgcgc
     7861 cttcgggacc tggcgcgtgg cttcagggtc gttctccccg ggtggcaccc ggcgcgtctc
     7921 atcgtcgtcg tctcccagag gacccgtcct gcggcgatcc tcgcggctct cttccggtct
     7981 atcgctcatc tctctccctt tacttcttat cgtcgcccgc taaatcttct tctgtagtac
     8041 agttacggat tctaccctgc gcgagtctcg ggcacccgca acgcccgcgc gctatcgcga
     8101 ttcgaggcag ctccaacgcg gatttttata catctggccg agaatcgggc tggatcccgg
     8161 cgcctggctt gtacgtcagg ccatatgcaa ccggtgcgcc cgcttcatat catggcgggt
     8221 gttgccagta tgtccccgcc cgggggcaga gtacagagcg aatatccgag gaggtaggct
     8281 tggacacggc tgacaggcag aacgtgttgc agcaggcaca agagaacaac gtacagttca
     8341 tccggctctg gttcacggac gtgctgggga cgctcaagag cttcgccata acggtcgagg
     8401 agctcgagga cgccctggac gggggcatgg gcttcgacgg ctcatcgatc acgggctacc
     8461 aggacatcga ggagtcggac atgatcgcca tgcctgaccc atcgaccttc caggtcatcc
     8521 cctggagtcc gcaagatgcg ccgacggcgc gaatgatctg cgacgtaagg acccccgacg
     8581 gcgaccccta cgtcggcgac ccgcgccacg tgctgcggcg cgcgcttgaa cgggcgaacg
     8641 agatgggctt cgacaagttc tactgcgggc ccgagctgga gttcttctat ttcaaggact
     8701 ccaagggtac ggagccgctc gactacggcg gctacttcga cctgacgacg ctcgacgcgg
     8761 cgacggcatt gaggcgtgag accgtccagt cgctgcagca gctcggcatc aaggtcgagt
     8821 actcgcacca cgaggtcggc atctcccagc acgagatcga cctccgcttc gacgacgcga
     8881 tgacgatggc tgacaaggtg atgacttaca agacggtcgt caaggaggtc gcgacccgcc
     8941 acggcgtgta cgcgacgttc atgccgaagc ccttgaggga ccagaacggc tcggggatgc
     9001 atacccatca gagcctgttc tcgggcggca agaacgcttt cttcgaccct gacaacgagt
     9061 actacctttc gagcgaggcg caggcgttca tcgcgggcca gctgaggcac gcgcgtgaga
     9121 tcagcataat cttcgcccag cacgtcaatt cctacaagcg cctggtcccc ggctacgagg
     9181 cccccgtcta catcgcctgg agccgccgca accgctccgc tctggtccgc gtccctcttt
     9241 accaccccgg ccaggagagg gccacgagga tggagcttcg ctgccccgac cccgcagcca
     9301 acatctacct ctgcttcgcc gcgcttctgc acgctggcct cgaaggcatc gagaagggct
     9361 acgagttgcc ggagccgatg gagcgcaacc tctaccatct gacgtccgag gagc
//
LOCUS       DATAIR010000169         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_425684, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000169 DATAIR010000000
VERSION     DATAIR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425684"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1169..1627
                     /locus_tag="VHM69_04080"
     CDS             1169..1627
                     /locus_tag="VHM69_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2739600.1"
                     /translation="MSAQENKQLALILIEALNARDMSLWSQYLSEDYSAEHPGVSVLL
                     DKTRSIAYHQRFVTAFPDIRFEVLGVLAEGDRVLVQWRGSGTHMERLATVTGETIPPT
                     RRRATVSGVMVTEVRDGKIVRGWFYWDQLSLLAQLGITEQPGLFLSAEGF"
     gene            complement(2061..2822)
                     /locus_tag="VHM69_04085"
     CDS             complement(2061..2822)
                     /locus_tag="VHM69_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX2739601.1"
                     /translation="MALENPALNERSFQRGIEETGAVPRGMTASGTYAITGLLFVIVV
                     VAAAFGWSQVEIITVGGREEALTPAWTWLAFLLTFVVAIVGAFAYRAAPVTSILYALG
                     EGSLLGIASRYYDLRFDGIVSQALVATLCIFLAVYLLYSLRIVRVTSRFATGVIAATG
                     GFLLLYLMVWLLSLFGVNFAFLNAPTPLGIGISVLIVILGALNLTLDFAFIEEAAAKG
                     APRFMQWYAAFGLMVSLIWIYVSVLRLIALLRARQ"
     gene            complement(3166..3390)
                     /locus_tag="VHM69_04090"
     CDS             complement(3166..3390)
                     /locus_tag="VHM69_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HEX2739602.1"
                     /translation="MGEKRDRDVEKVYSTSEFVAKLRRLADALEAGKRFEIQVAGERV
                     YVPAGAEFNVEHEREGEEEEIEFQLEWTNR"
     gene            complement(3774..>4057)
                     /locus_tag="VHM69_04095"
     CDS             complement(3774..>4057)
                     /locus_tag="VHM69_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739603.1"
                     /translation="DRPSCRPLSSEKVMESGGRQVSHIGTRTVYRIVVRDELSERYTA
                     AFEGMEMKIKNGQTILTGEIIDQPHLHGILDRIGALGLKLVSVEDLSEG"
BASE COUNT          838 a         1244 c         1147 g          828 t
ORIGIN      
        1 acgcagtcgg tgtcaaccgg gttcagaaca ctagagcggt ttgcggaaag tgtgaacctg
       61 aggtgagtaa aggctacagg catcagcaaa aagacctgaa gcctgaaacc tgtagcctga
      121 tgcctcctcc gaaggagtag ctcatccttt tcgagaaccg ccatagtgta agtctcattg
      181 tgttgggccc tccgacgtgc agtaacgcgt cacccgagag ccgttgtctg tgaatccgta
      241 acgatttcca agcttcgctg catctctaga gcagctgacg ccgagacggc tcggggtgag
      301 cgagcctgca gatggttggt gatcgcgtgg aggcgttcga gacggcgcac gtcgtcatct
      361 tgcccgtgcg tctcgagcca tcacctgccc atgggtcgtt ccgcactcac cgagtagacg
      421 tggctgatgc gtccacggat gaaccggaaa gtgtcgatcc ctccgacgtt gaggtcgccc
      481 cagccggcgt cccggacgca gtagccccac tgttcggtgg tgtgggcttc gactacccga
      541 tgcgtgagcc caggtcgcgt cgattaggtc ggcaaggccg ctggcatccc cggcctcggc
      601 gatggcgcgc ccgccgaagg gaatggtgac ctgcccggcg cagatcgtgg ccgcctggct
      661 caggtcacca ttccacagtg cggtccatcc ctcgaccaag gaggaattcg gatcatttcg
      721 aggcgttgta atcgtcatgc caaccatcct cgcgagcggt tgcgacaacg gagtgtcgga
      781 gttttccgaa gacctcgcgg tttggcccac cccatgcagc ctgagcgccg aacggactcg
      841 cggacctcaa tcaatgtccc cggtcagtac acctggtaac cgacctgccg cgcaggacag
      901 gcatgtgctg gaaggcgttc ttcctcttca gtgccttcgg cggtatagtg tgggtcgtgg
      961 cggtagtcct ggcagggtac ttcgtaggta attacacgct tactcggtaa aggagtgctc
     1021 cgacaggcgc aaatacggcg gttctcatgg ggattgctca acgcgcacgt agccacggtg
     1081 gttaaagtag cccaccgcgt cttcggccgg tgccgtcatg agcgccggtt ggctcaacat
     1141 caattcgggc ggaaggaggt cgactcttat gtcggcacaa gaaaataagc aactcgcgct
     1201 gatcctgatc gaagcgctca acgcccgcga catgagccta tggtcgcagt atctatcgga
     1261 ggactacagc gcagaacatc ccggcgtgtc cgtcctcctg gacaaaacca ggagcatcgc
     1321 ataccaccag cgcttcgtga cggccttccc agacatccgc ttcgaggtac tcggcgtcct
     1381 cgccgagggc gatcgcgtcc tcgttcagtg gagagggagc ggcacccaca tggaacggct
     1441 ggccaccgtc acgggagaga ccatcccccc gacccgccgg agggcgacgg tgtcgggggt
     1501 gatggttacc gaggtgaggg acggcaagat cgtacgcgga tggttctact gggatcagct
     1561 ctcactcctg gcccagctgg gcatcaccga acaacccgga ctcttcttgt ccgccgaggg
     1621 cttctagatc tggctcggat cgttatgatc agagccagat ctagcttcta aggctctatt
     1681 ctgggcacta cggtcagcaa ggctacgcaa ggggatccag catccaccct ggctaatgga
     1741 atgcaccggc aaccatgcat gatcccatga acgcgtttcc gacgatacac attccaggaa
     1801 tatgtgacaa cgggactaga acggcgaaca cgtagaggtt gtagatcgtg ccggatgcgc
     1861 tggattgcgg agaagggctg gcggcgcaac cacacaaatc tacagacgaa atcgcggccg
     1921 aagcgggttg tcgctgccgc tccgtaaacg ggagcctagt ttctcgagtc tggttttcga
     1981 cgtgctctga tctttagcta gttgcctcgg ttcgagtcaa tcttacttga ggaggacact
     2041 gctgatggag ccggcctctc ctattgtctg gcccgaagga gcgcaatcag cctcaggacc
     2101 gagacgtaga tccagatcag agagaccatc agcccgaacg cggcgtacca ctgcatgaac
     2161 ctcggcgcac ccttcgccgc tgcctcctcg atgaaagcga aatcgagcgt gaggttcagc
     2221 gcgcccagga tgacgatcaa gacgctgatc ccgatgccca atggtgtcgg ggcattcagg
     2281 aaggcaaaat tcaccccgaa cagcgacaac agccagacca tcaagtagag cagcaggaac
     2341 ccacccgttg ccgctatgac ccctgtggcg aatcgtgacg taactctgac gatgcggagg
     2401 ctgtaaagga ggtacacggc aagaaatatg cacaacgtgg ctaccagcgc ctgcgacacg
     2461 atcccgtcga acctcagatc gtagtagcgg gaagcgatac cgaggagcga accctcgccc
     2521 aaagcgtaca ggatggaggt gaccggggcg gccctgtagg cgaacgcgcc cacaatggcg
     2581 acgacgaacg tgagcaagaa ggcgagccag gtccaggccg gggtcaacgc ctcctcccgt
     2641 ccgcctacgg tgatgatctc gacctgcgac cagccgaaag ctgccgcaac caccacgatc
     2701 acaaacaaca gaccggtgat ggcgtaggtg ccggaggcgg tcatcccgcg gggcactgcg
     2761 cccgtctcct cgatcccccg ctggaaggaa cgttcgttaa gtgccggatt ctcgagggcc
     2821 attttctctc cttattcctg gtgcgctatg ggaggtgtgc cctcctcgat tagagcgcaa
     2881 tgaccagctt ctcaccactg gctgtggagt cgccgtactt ctgatcaact cgaggaaccc
     2941 gccgcaaaca cgagtacgcc gaggacatct gctttcctcg tctcttccaa gggctgcagc
     3001 gctaccgccg tatccatctc tggtacccat atacgagtag cgctcccaca gctccgccag
     3061 ctgttagcgc aactacttcc cgcaaaccat tctccatacc cctttactcc atgaaagtga
     3121 aacgtttgcg cattagccgg acaggacgtg aagctagtga gggggtcagc ggttagtcca
     3181 ctcgagttgg aattcgatct cctcctcttc cccttcccgc tcgtgctcga cgttgaactc
     3241 ggccccggcg ggcacgtaga ccctctcccc agccacctgg atctcgaatc gcttacctgc
     3301 ttccaaagcg tcggccagcc tgcgcagctt cgctacgaat tcagaggtgg agtagacctt
     3361 ctcgacgtcc ctatcgcgtt tttcgcccat caaacctcca atgttcgctt aacactcgcc
     3421 ctgctggatt caattggaga atccgctttc tcgatgtctc tctgtgcctc agtcatccga
     3481 ctttctccag accaagaggg acacacccat ggcaggatgg gtgtgcgaga tcaactcgaa
     3541 gccattctgg tggcgagcgt tcatgaggtc ctgcaaccgg aggcagtcca caccgtaggt
     3601 gcccagtcca acacgatcga tcttgtaggg accttcgacg tgtccgggtt ctcgagtttg
     3661 cgctacctgc tgatgctgcc tcccgaacat ctcggttcct ccttcgtccg tcttctcttg
     3721 attagggtgg tacgtaggac gatcccactc tctgagtttg gcggctcatt gcctcagcct
     3781 tcggacagat cctcaacgct caccagcttg agccccaaag cgcctatacg gtcgaggatg
     3841 ccatggaggt gaggctgatc gatgatctcc ccagtaagaa tcgtttgccc gttcttgatc
     3901 ttcatctcca tcccttcgaa tgccgcagtg tatcgctcac tgagctcgtc acgaaccacg
     3961 atccggtaca ccgtcctcgt acctatgtgg ctcacctgtc tgccaccgct ttccataact
     4021 ttctccgagc ttagaggacg gcacgacggt ctgtcat
//
LOCUS       DATAIR010000170         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_327824, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000170 DATAIR010000000
VERSION     DATAIR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327824"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHM69_04100"
     CDS             <1..622
                     /locus_tag="VHM69_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2739604.1"
                     /translation="EPKAPDMNLTVEDLDLEAIAAAPLFWMTGTGLSDEPSRTATLAA
                     MEQRPSGITVFDLDYRPMLWESEEEAGYWYREALRHATVAVGNQSEAEVAVGTGDPLD
                     ASEALLELGLDLAIVKRGPDGVLARTKDGVAEAPPIEVEVVNGLGAGDAFGGALCHAL
                     LSGWDAERTIRYANAAGAIVASRLACADDMPTREEVEELLEKSAGV"
     gene            615..1517
                     /locus_tag="VHM69_04105"
     CDS             615..1517
                     /locus_tag="VHM69_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2739605.1"
                     /translation="MSDLVERLTRIRAENPAAVMEALAARERRPLLGESGTMFLLAVD
                     HPARGSLRVGDDPMAMSDRGDLLERTMIALDRPGVDGLMASPDIVDDLALLGALDGKV
                     VIGSMNRGGLAGAVFELDDRFTGYTAGAIAAANLDGGKMLLRIADDVHDTLPTIAACA
                     EAVDALASRGLMAMIEPFASRREDGKVKNVLEPDPTIRAIGITSALGPTSAYTWLKLP
                     VVPEMERVMAATTLPTLLLGGDPGSDAQTTFDGWRRAMKIPQVRGLVAGRALLYPQDG
                     DVAAAVDSAAEIVAASSGRETEVR"
     gene            1519..>2853
                     /gene="iolD"
                     /locus_tag="VHM69_04110"
     CDS             1519..>2853
                     /gene="iolD"
                     /locus_tag="VHM69_04110"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX2739606.1"
                     /translation="MGTIRLTAAQALVRFLTQQYVERDGIEHRFFTGCFGIFGHGNVA
                     GVGQALQQYADGLPYYQARNEQAMVHTASAFARQKNRLQAFACTSSIGPGATNMVTGA
                     ALATINRLPVLLLPGDVFASRGPDPVLQQLEAPHDGTLSVNDSFRPVSRYWDRVVRPE
                     QVIPAALAAMRVLTNQAETGAVTLAFPQDVQTEAFDYPDEFFEKRVWHVGRPLPDASA
                     LERAGDMIRAARRPMIVAGGGVIYSEATDALRRLSGRTGISVGETQAGKGSMPYDHPC
                     SLGAVGATGTSAANRAAREADLVIGVGTRWSDFTTASKTAFQNPDVRFVNINVADFDA
                     NKHAGLGLVGDARATLEALDDALAGYEVDQGYREQNTQASREWDREVERLYSLDHGLL
                     PAQSEVIGAVNSFSEAEDVVVCAAGSMPGDLHKLWRTRDPKGYHVEYGYSCMG"
BASE COUNT          496 a          929 c          978 g          450 t
ORIGIN      
        1 cgagcccaaa gccccggaca tgaacctgac cgtcgaggac ctcgacctcg aagccatcgc
       61 cgccgcgcct ctgttctgga tgacgggtac tggcctctcc gacgagccga gccgcacggc
      121 gaccctcgcc gcgatggagc agcgcccctc cggcatcacc gtcttcgacc tcgactacag
      181 gcccatgctc tgggaatccg aagaggaggc cggatattgg tacagggagg cgctgcggca
      241 cgccacggtc gcggtcggga accagtcaga ggccgaggtg gccgtgggta caggcgaccc
      301 gctcgacgcc tccgaggcgc tcctggagct ggggctcgac ctcgccatcg tcaaacgggg
      361 acccgacggt gtgctcgccc ggacgaaaga cggcgtcgcc gaggcacccc cgattgaggt
      421 cgaggtcgtc aacgggctcg gggcgggtga cgcgttcgga ggggcgttgt gccacgcgct
      481 gctctctgga tgggacgcgg agaggaccat tcgttacgcg aacgccgcgg gggccatcgt
      541 cgcctcgcgt ctggcgtgcg ccgacgacat gcccacccgc gaagaggtgg aggagcttct
      601 ggagaagtcc gccggtgtct gatctcgtcg agaggctgac caggatcagg gctgagaacc
      661 cggccgctgt catggaggcg ctggccgcgc gcgagcggag accgctgctc ggcgagagcg
      721 gtaccatgtt cctgctggcc gtcgatcacc cggcgcgcgg cagcctcagg gtaggggatg
      781 acccgatggc gatgtcggac aggggtgatc tcctcgagcg aaccatgatc gccctcgacc
      841 gtccaggcgt ggacgggctc atggcgagcc ccgacatagt ggacgacctt gctctactcg
      901 gggcgttgga cggcaaggtc gtgatcggct cgatgaacag gggcgggctc gccggagccg
      961 tcttcgaact cgacgaccgt tttacaggat atacagccgg ggccatagcg gcggccaacc
     1021 tcgacggcgg caagatgctc ctcaggatcg ccgacgacgt ccacgacacg ctcccgacta
     1081 tcgcggcctg cgcagaggcg gtagacgccc tggcgtcgcg gggcctgatg gcaatgatcg
     1141 aaccgttcgc cagccggcgt gaggacggca aggtaaagaa cgtacttgag ccggatccca
     1201 cgatcagggc catagggata acatccgccc tcggcccgac ttccgcctac acctggctga
     1261 agctgcccgt ggtcccagag atggagcggg tgatggcagc gacgaccctt cctacgctgc
     1321 tgctcggcgg cgaccccgga tcagacgccc agacgacgtt cgatggctgg aggagggcga
     1381 tgaagatacc ccaggtgcgt ggcctcgttg ccggacgtgc cctgctgtac ccgcaagacg
     1441 gcgacgtcgc ggccgcggtg gactccgcgg cggaaatagt cgcggcaagt tcgggacgag
     1501 agacggaggt gcgttgaagt gggcaccata cggttgaccg cggcccaggc gctggttcgt
     1561 ttcctgacac aacagtatgt ggagcgcgat gggatagagc atcgcttctt caccgggtgc
     1621 ttcggtatct tcggccacgg caacgtcgcg ggcgtgggcc aggcgctcca gcagtacgcg
     1681 gatggcctcc cttactacca ggcccgcaac gagcaggcga tggttcacac ggcctcggcc
     1741 ttcgcccgcc agaagaaccg actccaggcg ttcgcctgta cctcctccat cggtccaggt
     1801 gcgaccaaca tggtcacggg agccgcgctc gccaccataa accggctgcc cgtgttgctc
     1861 ctgccgggcg acgtcttcgc ctctcgcggc cccgaccccg tcctgcagca gctcgaagcg
     1921 ccgcacgacg ggacgctctc cgtaaacgac tcgttcaggc ccgtgtcgcg gtactgggat
     1981 agggtggtgc gccccgagca ggtgatcccc gcggcactcg cggccatgcg cgtgcttacg
     2041 aatcaggccg agaccggcgc cgtcaccctc gctttccccc aggacgtgca gaccgaggcc
     2101 ttcgactatc ccgatgagtt cttcgagaag cgggtatggc acgtcgggcg tcctctgcct
     2161 gatgcaagtg ctctggagcg tgccggagac atgatccggg ctgcccgcag gccaatgatc
     2221 gtcgccggag ggggtgttat ctactcggag gccaccgatg cgctgcgtcg tttgagcggg
     2281 cggacgggca tctccgtcgg cgagacgcag gcaggaaagg gctccatgcc ctacgaccac
     2341 ccctgctcac tcggtgccgt gggcgcgacg ggcacctcgg ccgcgaaccg cgcagcccgc
     2401 gaggccgatc tcgtcatagg ggtcggcacc cgctggagcg acttcacgac ggcctcgaag
     2461 acggctttcc agaacccgga cgtacgtttc gtcaacatta acgtcgccga cttcgacgcc
     2521 aacaagcatg ccggcctcgg cctcgtaggc gacgcccgcg ccacgctcga agctctcgac
     2581 gatgccctcg ccggttacga ggtggatcag gggtacaggg agcagaacac acaggcatcg
     2641 agggagtggg accgggaggt cgagcggctg tattctctgg atcacggtct gctgcccgca
     2701 cagagcgagg tcataggcgc ggtcaactcg ttctcagaag ccgaggacgt ggtggtatgc
     2761 gcggcgggtt ctatgccggg cgaccttcac aagctatgga ggacgcgcga cccgaaggga
     2821 taccacgttg agtacggcta ctcgtgcatg ggg
//
LOCUS       DATAIR010000171         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_426010, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000171 DATAIR010000000
VERSION     DATAIR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426010"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..706)
                     /locus_tag="VHM69_04115"
     CDS             complement(53..706)
                     /locus_tag="VHM69_04115"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2739607.1"
                     /translation="MKAAVSLWSADLLEVGTALDRLTGVADAFHVDVMDGHFAPDLLF
                     GPDFVRAVRKRTRGVVDVHLIVSEADRWVKPFAEAGADVLTVYPQSCPDLRGTLEEIR
                     RLGVSPGVSIDLGYPLERVRDAVLGLADRIVVMGTETGVKGVGLEPEALERVREVVRW
                     RGRPEIYVDGGIRRHTVPEIARAGADGVVPGSLVFGSESWEEAVRWVQSQDARGATR"
     gene            complement(703..1557)
                     /locus_tag="VHM69_04120"
     CDS             complement(703..1557)
                     /locus_tag="VHM69_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739608.1"
                     /translation="MPAFERGGLTFNFLNKGDTLGWRPFIFQHGLGGDVSQPAGVFPP
                     PRGFRLISLDCRGHGGTRPLGPADKLSFSSFADDVVALMDHLGLQRAVVGGISMGAGV
                     ALNLASRYPKRVAGLVLSRPAWLDSPRPENLKAYSVIARLLTEHGARLGRELFEGTEE
                     YAELESAAPGAGRSLASSQFGRPRAREYAPVLESLAGDAPGRDLAQWRDDVRAPALVL
                     ASWLDPTHPHSYAEILARTLPSSQFAELTPKAEDEGRHAEDARAAIAGFLGRVFPPAA
                     AQEEAVGR"
     gene            complement(1557..2414)
                     /locus_tag="VHM69_04125"
     CDS             complement(1557..2414)
                     /locus_tag="VHM69_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2739609.1"
                     /translation="MRLGIFARTFSRPSLEEVLDAVAGYGLHETQFNMSVAGLPSMPE
                     EIEPALADRVREAAAGRKVSVAAVSGTFNMAHPNQEIRRDGLRRLCVIAGSCERLGTS
                     TITLCTGTRDRENMWCRHPDNGTPEAWQDMLATMQEALETAEEHGVTLAFEPEIGNVV
                     DSAQKGRRLLDEMRSPRLKVVMDAANLFDAEDPARGLSRSKEILYEAFELLGDDIVIA
                     HAKDVRASGEIVAAGKGDLDYDLYLEHLFEAGYHGPLILHGLAEEEVAGSLTFLRGKL
                     AGTAEEAGR"
     gene            complement(2416..3489)
                     /locus_tag="VHM69_04130"
     CDS             complement(2416..3489)
                     /locus_tag="VHM69_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2739610.1"
                     /translation="MSEEQRALRVGVLGAGPISQAAHFEACRKAENAELYAICDVAED
                     LLERVAAIHAPENTYGDYDAMLAAPDVDAVIVAIADQFHVPMALKALSAGKHVLVEKP
                     MGVTVEECRRLRDAAEASGLVVQVGTEKRFDEGIAFARDFIREQMGEMIALKAWYCDS
                     THRYTMTEALQPMIETSKKAKRPEGDPKADLRRYYVLGHASHLLDTARFLGGQIESIR
                     TRLVEKFGAYCWFSEVEFADGSAGHLDLTLKIRMDWHEGFQVYGEHGSVIGKTYNPWY
                     YKASDVECFSEKDGQYHRPLGADGHFFRRQIEGFAETILDGAPMRGASAEDGLAAMRG
                     MVAMARSVETGEVVRLAEVEGGV"
     gene            complement(3798..>4338)
                     /locus_tag="VHM69_04135"
     CDS             complement(3798..>4338)
                     /locus_tag="VHM69_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739611.1"
                     /translation="GGLFLAWEDSATERAPLWLGLLQNTVLISYAAVPIAVGVAVLRY
                     RLYDIDLLINRTLVYGPLTAMLALMYVGSVVGLQALFRALSGQESTLAVVASTLAIAA
                     LFNPLRRWVQALVDRRFYRRKYDAAKTLATFNTRLREVTDLDSLRDEVVGVVRETMQP
                     AHASLWLRTDSTDAEDVPG"
BASE COUNT          712 a         1526 c         1374 g          726 t
ORIGIN      
        1 atcacagggc cggaggtgcc gactggatgc ggcccctccg cgtccgacac tcctaccgcg
       61 tcgccccgcg ggcatcctgc gattgcaccc accgtaccgc ctcctcccag ctttcagagc
      121 cgaagacgag cgagcccggc acgaccccgt ccgcgccggc ccgcgctatc tcgggcaccg
      181 tgtggcgccg gatgcccccg tccacgtaga tctcggggcg cccgcgccag cgcaccacct
      241 ccctgacgcg ttcgagggcc tcgggttcca gaccgacccc cttcacgccc gtctcggtac
      301 ccatgacgac gattcggtcg gcgagcccca gaacggcgtc gcgcacgcgc tccaacgggt
      361 agccaagatc gatggacacc cccgggctta ccccgaggcg tctgatctcc tcgagcgtgc
      421 cccgaaggtc cggacacgat tgcgggtaaa cggtcaggac atcggctccc gcctcggcga
      481 agggcttcac ccatcggtcc gcctccgaga cgatcagatg cacgtctacc acgcccctgg
      541 tgcgtttcct caccgcccgc acgaaatccg gaccgaacag gagatcgggc gcgaagtggc
      601 cgtccatcac gtccacgtgg aacgcgtccg ccacccccgt cagccggtcg agggccgtgc
      661 ccacctcaag gaggtctgcc gaccagagcg agaccgccgc cttcatcgtc ccaccgcctc
      721 ctcttgcgcc gccgcaggcg ggaagacgcg gcccaggaag cccgcgatgg cggctcgcgc
      781 gtcctccgcg tggcggccct cgtcttcggc cttcggggtc aactcggcaa actgcgacga
      841 aggcagcgtg cgcgccagga tctcggcgta gctgtgaggg tgggtcgggt ctagccacga
      901 tgcgagcacg agcgccgggg cgcgcacgtc gtcccgccac tgcgccaggt ccctgccggg
      961 agcgtctccc gccaacgact cgaggaccgg cgcgtactcc cgggcgcgcg gcctaccgaa
     1021 ctgcgaggag gccagcgagc ggcccgcccc gggagcggcg ctctcgagct ccgcatactc
     1081 ctcggttccc tcgaagagct cccgccccag cctggcgccg tgctccgtca acagccgggc
     1141 gatcaccgag tatgccttga ggttctcggg tctcggcgaa tcgagccacg ccgggcgcga
     1201 gaggaccagc cccgccaccc tcttggggta acgtgaggcc aggtttagcg ccaccccggc
     1261 gcccatagag atgcccccga ccacggcacg ctggagcccc aggtggtcca tcagcgccac
     1321 gacgtcgtcg gcgaaggacg agaaggagag cttgtccgcg gggccgaggg ggcgcgttcc
     1381 cccgtggcct cggcagtcca gtgagatcag ccggaaacca cggggcggcg ggaagacgcc
     1441 ggcgggttgg gagacgtcgc ccccgaggcc gtgctggaag atgaagggcc gccaccccaa
     1501 ggtgtccccc ttgttcagga agttgaaggt caggccgcca cgctcgaagg cgggcatcag
     1561 cgtcccgcct cctcggccgt accggccagc tttccccgga ggaaggtcag gctgccggcc
     1621 acctcctcct cggccaggcc gtgcaggatc agcggcccgt ggtagccggc ctcgaacaga
     1681 tgctccaggt acagatcgta gtccagatcg cccttgcccg cggccacgat ctcgcccgac
     1741 gccctcacgt ccttggcgtg ggcgatgacg atatcgtcgc ctagcagctc gaacgcttcg
     1801 tacaggatct ccttcgagcg ggagagtccg cgggcggggt cttcggcgtc gaagaggttg
     1861 gctgcgtcca tgaccacctt gaggcggggc gagcgcatct cgtcaaggag gcgtcgtccc
     1921 ttctgtgccg agtcgacgac gttgccaatc tcgggctcga aggcgagcgt gacaccgtgc
     1981 tcctctgccg tctccagcgc ctcttgcatc gtggcaagca tatcctgcca cgcttcgggc
     2041 gtcccgttgt ccgggtgccg gcaccacatg ttctccctgt ccctggtacc cgtacagagt
     2101 gtaatcgtgg acgttccgag cctttcgcag gaacccgcga tcacacagag gcgacgcagt
     2161 ccgtcccggc ggatctcctg gttagggtgg gccatgttga aggtgcctga gacggcggcc
     2221 accgagactt tgcgcccggc ggcggcctcc cgcaccctgt ccgccagggc cggctctatc
     2281 tcttccggca tggagggcag accagccacc gacatgttga actgcgtctc gtgcagaccg
     2341 tacccggcca cggcgtcaag gacctcctcg agcgaggggc gggagaaggt cctggcgaag
     2401 atccctaacc gcaccctaga cgcctccctc gacctcggcg agcctcacga cctcgcccgt
     2461 ctccaccgag cgggccatcg caaccatccc gcgcatcgcc gcgagaccgt cctccgcgct
     2521 cgcaccgcgc atcggggcgc cgtcgagtat cgtctccgcg aacccctcaa tctgcctccg
     2581 gaagaaatgg ccgtccgcgc ccaggggacg gtggtattga ccgtccttct ccgagaagca
     2641 ctccacgtcg ctcgccttgt agtaccaggg gttgtaggtc ttcccgatca cgcttccgtg
     2701 ctcgccgtag acctggaagc cctcgtgcca gtccatgcga atcttcagcg tcaggtcaag
     2761 gtgccccgca ctcccgtcgg cgaactcgac ctcggagaac cagcagtacg cgccgaactt
     2821 ctcgacgagc ctggtgcgga tggattctat ctgcccgccg aggaaacggg ccgtgtccag
     2881 caggtggctc gcgtgcccga gcacgtagta gcgtcttaag tcagccttcg ggtccccctc
     2941 gggtcttttt gccttcttgc tcgtctcgat catcggctgc aaagcctcgg tcatggtgta
     3001 gcggtgggtc gagtcgcagt accaggcttt aagagcgatc atctcgccca tctgctccct
     3061 gatgaagtcg cgggcgaatg cgatgccctc gtcgaagcgc ttctcggtac cgacctgcac
     3121 gacgagcccg gacgcttcgg cggcgtcacg cagcctcctg cactcctcga ccgtgactcc
     3181 catcggcttc tccaccagga cgtgtttgcc tgccgaaagg gccttgaggg ccatcgggac
     3241 gtggaactgg tcggcgatgg cgacgatcac cgcgtcgacg tcgggggcgg cgagcatggc
     3301 gtcgtagtcg ccgtaggtgt tctcgggcgc gtggattgcg gccaccctct caaggaggtc
     3361 ctcggccacg tcgcagatgg cgtagagctc ggcgttctcc gccttgcggc aggcctcgaa
     3421 gtgcgcggcc tgcgaaatcg gcccagcgcc tagcacccct accctaagcg ctctctgttc
     3481 ctcgctcatg cgacaagaat actccgggcc gctatctgcc gcacgagcac ttcggtaaac
     3541 cccctctacg cggagctcgt cctgcccagc gtggcggcga agcggacctt cgggaccatt
     3601 gccgcatgtc gcggcatctt gccgcttttc cgtccagggt cagtgtcgtg caggcgttct
     3661 cagcgtcgcc acagtgtgaa agcctcgacg gctcccggtc gaggactacc cacaaacgga
     3721 gggagggaag gtgttgcaat ccggtcgtag gggtggaatc aggagcaggc gaacactttg
     3781 atcggaggct ccttcagtta gccgggtacg tcctccgcat ccgtggagtc ggtacgcagc
     3841 cacagcgagg cgtgggcggg ctgcatggtc tccctgacta cccctaccac ctcgtccctc
     3901 aaggagtcga gatcggtcac ctcgcgcaac cttgtgttga aggtggcgag cgttttggcg
     3961 gcatcgtact tccttcgata gaatcgccgg tctaccagcg cttgcaccca ccgacgtaga
     4021 gggttgaaaa gagctgcgat cgccagagta gatgcgacga ccgccagggt ggactcctgg
     4081 ccgctgaggg cacggaagag ggcctgcagg ccgaccacgc ttccgacgta cattagagca
     4141 agcatcgcgg tgagaggccc gtagacgagg gtgcggttta tcagaagatc tatgtcgtag
     4201 aggcgatacc tcaacacggc gacgcccacg gcaatcggga ccgccgcgta gctgatcagg
     4261 accgtgttct gcaggagccc gagccacagc ggtgctcgtt cggtggccga atcttcccat
     4321 gccagaaata gcccgcca
//
LOCUS       DATAIR010000172         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_189506, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000172 DATAIR010000000
VERSION     DATAIR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189506"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VHM69_04140"
     CDS             complement(<1..737)
                     /locus_tag="VHM69_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX2739612.1"
                     /translation="MSISASPSGGETSQEFHEFVCDVALRAGELQLSRYDRPGEIREK
                     APKDLVTEVDLLCEDLMVSRIRERYPEDAILSEERGGEISDTGRTWLLDPVDGTANFA
                     RSNPLFCSCVTVLRDGEVAHAAVAAPRLGDLYHARRGAGAFRDSAGGQSTPLKVGDAE
                     SLEYAFVGADLSFTGLPGHTDGSGPFGRIFDAAWQLRALGSAGIRGAWVAAGYLDVSV
                     GTRNTVWDYAPTALLVSEAGGVATDLSG"
     gene            complement(721..1824)
                     /locus_tag="VHM69_04145"
     CDS             complement(721..1824)
                     /locus_tag="VHM69_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2739613.1"
                     /translation="MSSESTMMLTARTDELLSRGRSQGFVSAEEVADLLQAGDLSATE
                     IEEFQATLEEEEISVVETASPEASGTNGAAPTNGHAPETPTLQIAHAVATGDSIRMYL
                     AEIGRVKLLTHADEIRLANGIARGCKRSKDRLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDPTKGFKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKF
                     HRTHRRMTQTLGREPLDEEIATELDVPVAEILRLREISQRAISLETPVGEDDSSLLGD
                     FLEDAATVTPTDAVSESLLKLHLREALNELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLR"
BASE COUNT          305 a          611 c          622 g          376 t
ORIGIN      
        1 ccggacaggt ctgtggcaac cccgccggcc tcggagacga ggagcgctgt gggggcgtag
       61 tcccacacgg tgttgcgggt gccgacggag acatcgaggt atccggcggc cacccaggct
      121 cctcgaatgc ctgcgcttcc cagggccctg agttgccatg cggcgtcgaa gatccgcccg
      181 aaaggcccgc tgccatccgt gtgcccgggg agtcctgtga aagagaggtc cgcgccgacg
      241 aatgcgtact ccaggctctc cgcgtcaccg accttgaggg gggtgctctg gcccccggct
      301 gaatcccgga aggcgcctgc cccgcgcctg gcgtgataga gatccccgag gcgcggggca
      361 gcgaccgcgg cgtgggccac ctcgccatcc cgaagcaccg tgacgcagga acagaagagg
      421 gggtttgacc tggcgaagtt ggcggttcca tccaccgggt cgagcaacca ggtacgtccg
      481 gtatcggaga tctcaccgcc gcgttcttcc gagaggattg cgtcctcggg gtagcgttcc
      541 ctgatgcgcg agaccatcag atcttcgcat agaaggtcaa cctcggtcac gaggtctttg
      601 ggggctttct cccggatctc acccggacgg tcgtagcggg agagttgtag ttctcccgcc
      661 cgcagggcca cgtcgcagac gaactcgtga aattcctggg aggtttcccc cccggaaggg
      721 ctagcggaga tactcacgca ggttctgggt gcggcgctgt tcgcggagta ggttgagggt
      781 cttcatctgg atctgacgca cgcgctcgcg ggtgatgtcg aactcgcggc caacctcttc
      841 gagggtgcgt ggcctgtcgt ccttcatccc gaagcgcaac tcgatgatct ggcgttcccg
      901 ctccggaagc tcgttgagcg cctcgcgcag gtggagcttc aagagcgatt ctgagacggc
      961 gtccgtcggg gtgacggtgg cggcgtcctc caggaagtcc cccagcagcg atgaatcatc
     1021 ctcgccaacg ggggtctcaa ggctgatggc gcgctggctg atctcacgca gcctcagtat
     1081 ctcggcgacg ggaacgtcga gctccgtggc tatctcctcg tcgagcggct cgcggccgag
     1141 cgtctgggtc atgcggcggt gggtgcggtg gaacttgttt accttctcca ccatgtggac
     1201 ggggatgcga atggtgcgcc ccttgtccgc tatggcgcgg gtgatggcct gacgaatcca
     1261 ccacgtagcg taggtcgaga acttgaaacc cttggtcggg tcgaacttct cggcggcccg
     1321 gatcaggccg aggttcccct cctggatgag atccagaaac gacaccccgc gattcctgta
     1381 cttcttggcg atggagacaa ccagcctgag gttcgcctcg acgagccggt ccttgctgcg
     1441 cttgcagcct cgggcgatgc cgttggcgag ccggatctca tcagcgtgcg taagcagctt
     1501 gacccttccg atctccgcga gatacattct gatggagtcg ccggtcgcga cggcgtgtgc
     1561 gatctgaagc gtcggtgtct ccggcgcgtg gccgttggtc ggcgcggccc cgttggttcc
     1621 tgaggcttcg ggagatgcgg tctctaccac cgagatctcc tcctcttcga gcgtagcctg
     1681 gaactcctca atctcggtgg ccgacaggtc gcccgcctgc aacaggtcgg caacctcctc
     1741 cgcactgacg aagccttgac ttctcccccg gctcaaaagc tcgtcggtgc gcgccgttaa
     1801 catcatggtg ctctcgctcg acattcgtct cctctccggc cgccagaccg atatgagttt
     1861 gctacaaccc tccgccgggg cttcccgaag gccaccgatt gttaacgttc catg
//
LOCUS       DATAIR010000173         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_51020, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000173 DATAIR010000000
VERSION     DATAIR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51020"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VHM69_04150"
     CDS             complement(<1..674)
                     /locus_tag="VHM69_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2739614.1"
                     /translation="MEVQISEGHVATYEVIGGGGEPLLSFVGGPGLSAKLMRPDAELF
                     AEQFCCYLIDPHGSGGSTPPQDETSYDPAGHARFYDEVRKALDLGPVNVHGESFGGTV
                     ALTYAALFPESTTRCIAVSAFAVGEELDEEEGGDAAAEMEAALARHADAEWYPEAREV
                     WDTWTQRARAATDPEEIRDMLATVFPLYTAYPDRPDVRKALDEARAMLEVDLHAVKVW
                     ESGLYQR"
     gene            1111..2715
                     /locus_tag="VHM69_04155"
     CDS             1111..2715
                     /locus_tag="VHM69_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739615.1"
                     /translation="MVAVIQPCDVGVIKSTRGAAPCQQSSRPWVIAAASLGSGMAFLD
                     STVMNVALPAVQTDLELSAREVQWVFGAFALVVAAFLLVGGSLGDRYGRQRLFVLGAA
                     IFAVASAWCALASGPGQLIAARAVQGTGGALLVPASLAIIGASFEGRLKAKAIGLWGA
                     FSGAAMAVGPVLGGWLVEVVSWRAAFLITPAMALVAIPIALRHVPESRDPEAHRLDLI
                     GAVLATAGLGGLVYGLIESSASGFADPVVLAALLLGALALSAFVFAERHENDPMVPPS
                     LFRSRDFDGATLVTLLFYMALTGSLYFVPFLMMQVHGYSALAAGSVFLPFVAMALLLG
                     RLSGYIMARFGARMPLVVASLAIAMGFVLFALPGVEHGSYWTSFFPAMVVQGFGMALA
                     ITPLTTVALNSVEGEHSGMASGVNNAAARVAAPLAVAVLGVFVFGVFSANLDARLDSM
                     NLPGEVRGEMEAAKAHLGAAEVPNGVDAGTAAHLERAIDESFVAGFRIVMLICIGFAL
                     ASATAAVLLVGDRRLQIASSEPYPSD"
     gene            3637..3843
                     /locus_tag="VHM69_04160"
     CDS             3637..3843
                     /locus_tag="VHM69_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2739616.1"
                     /translation="MSRGTVKWFSGEKGYGFITPDEGGEDLFVHYSAIEGSGFRSLEE
                     GERVSYEPTRGRKGEQAENVRRVE"
     gene            4119..4340
                     /locus_tag="VHM69_04165"
     CDS             4119..4340
                     /locus_tag="VHM69_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739617.1"
                     /translation="MQWVGDGEGTAVRVRIGYGFPPGRTFRRCARQPGAPATPATATQ
                     VGACREAPVILTVDAAAMHAAGDLSGWPG"
     gene            complement(4337..>4558)
                     /locus_tag="VHM69_04170"
     CDS             complement(4337..>4558)
                     /locus_tag="VHM69_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX2739618.1"
                     /translation="ILVTAYTDPSWTPLFVAIKGLVTEVGGLMTHGAVIAREYGLPAV
                     VGVEHATRLIRDGQRIRVHGTDGYVEILP"
BASE COUNT          777 a         1386 c         1472 g          923 t
ORIGIN      
        1 cgctggtaga ggccgctctc ccacaccttg actgcatgca ggtccacttc gagcatcgca
       61 cgggcttcgt ctagcgcctt gcgcacatca ggacggtcgg gataggcggt gtagagcgga
      121 aagacagtgg cgagcatatc cctgatctcc tcaggatcag ttgcagcccg cgcgcgctgc
      181 gtccaggtat cccagacctc gcgagcctcg ggataccatt ctgcatctgc atgtcttgct
      241 aacgcggcct ccatctccgc agctgcatcc ccgccttctt cttcgtcgag ttcttccccc
      301 acggcgaagg cgctaacggc gatgcagcgg gtggtggact ctggaaatag ggctgcgtac
      361 gtgagcgcga ccgtgccgcc aaaagactcc ccatgcacgt tgaccggacc gaggtccagc
      421 gctttacgca cctcgtcgta gaagcgtgcg tggcccgcag gatcgtagga ggtttcgtct
      481 tgcggcggtg tggagccgcc cgagccgtgc ggatcgatga ggtagcagca aaattgctcg
      541 gcaaagagtt cggcgtccgg gcgcatgagc tttgccgaaa gcccgggtcc gccgacgaag
      601 gagagcagtg gctcaccgcc accaccgata acctcgtagg tcgcaacgtg accttcggag
      661 atttgcactt ccatgcgcct cagcccttct cagggatcgt ggaaaccgtc aaacgcccgg
      721 atagcaactt cctcaccaac acttcgattc tcctgcccct cggagacagc tagccagtat
      781 agtaacaagt gtggagtacc gaattcaccc cgacaggatc tgttcctaga cattccatcg
      841 acgaaccctt gcagaacgct tctcttcccc gccattggct aaataaagtc catcgctggc
      901 ttcctcattc acccaggtgc ctagaaaaga tagaaaagag gagttctcag aacttttcca
      961 aggaaagtcc ttcgaagatc actcatttgg accatgcatg attctcagtg tcctgttacc
     1021 ttctccgatg gtaagcgtca ggtggtgacc agatcgcggg ctgttgcgaa tgagcacatc
     1081 gagtagcagg gctatacggg tgaagccata atggttgccg tcattcagcc gtgtgatgtg
     1141 ggcgtcatca agagtacgcg gggtgcagcg ccgtgccaac agtcctcgcg gccctgggtg
     1201 atagcggccg cctctttagg ctccggcatg gccttcctcg acagcacggt gatgaacgtg
     1261 gcactgcctg cggtgcagac ggacctcgaa ctgagcgcac gcgaggtgca gtgggtcttc
     1321 ggagccttcg ccctggtggt tgctgctttc ttgctcgtcg ggggctctct cggagaccgc
     1381 tacgggaggc agcgcctctt cgtcctgggc gcggcgatct tcgctgtcgc ctctgcctgg
     1441 tgcgccctcg cctccggtcc ggggcaacta atcgctgctc gggccgtgca gggcacgggc
     1501 ggagcgctcc tcgtgccggc ctcgctggcg atcatagggg catctttcga ggggaggctc
     1561 aaggcgaagg ccatcgggct ttggggggcg ttcagcggcg cggcgatggc tgtgggaccg
     1621 gttcttggcg gctggctggt cgaggttgtc tcctggcgtg ccgctttcct gatcacccct
     1681 gcgatggccc tcgtggccat acccatcgcg ctgcggcacg tgcccgagag ccgggatcct
     1741 gaggcccaca gactggacct catcggcgcg gtcctcgcaa cggccggact cggaggtttg
     1801 gtctacggtc tgatcgaatc ctctgcttcg ggtttcgccg atcccgtggt gcttgccgcc
     1861 ttgctccttg gcgccttggc actttcggcc ttcgtctttg ccgagcgaca cgagaatgat
     1921 cctatggtgc cgccctcgct gttccggtcg agggatttcg acggtgcgac ccttgtcacg
     1981 ctgcttttct acatggccct taccggctcg ctgtattttg tacctttcct gatgatgcag
     2041 gtccacggct attcggccct ggccgcgggc agcgtgttcc tgcctttcgt ggccatggct
     2101 ctgttactcg gtcgcctgag cggatacatc atggcccgct tcggggcgag gatgccgcta
     2161 gtagtagcct cgctcgccat tgcgatggga ttcgtgctgt tcgccctgcc gggcgtggag
     2221 cacggctcct actggacctc gtttttccct gcgatggtcg tccagggttt cgggatggcg
     2281 ctggccatta ccccgcttac cacggtggcc ctcaactcgg tggagggtga gcactccggg
     2341 atggcctcgg gggtgaacaa cgccgcagcc cgggtagcag cgcctctggc ggtcgccgtt
     2401 ctgggcgtgt tcgtcttcgg ggtattctcc gcgaacctgg acgcccgcct cgacagcatg
     2461 aacctgcctg gtgaggttcg cggcgagatg gaggcggcga aggcccatct cggggcggca
     2521 gaggtgccga acggggtgga cgcggggacg gcggcgcacc tcgagcgcgc catagacgag
     2581 tccttcgtcg ccgggtttcg aatcgtgatg ctgatctgta tcgggttcgc gcttgccagc
     2641 gccaccgccg cggtgctcct ggtcggtgat cggaggttac agattgcgag tagtgaaccc
     2701 tacccgtcgg actagcatag gttggctttg ctcgaagcgc gggcctttgc ccacgggcat
     2761 atattgagac agcggtagga tcgagatggt tacaccctgt tccaaaatcc gagtttgtta
     2821 ctctcgaggg tgttggatcg gggctatgct aacttccaag aaccccctca cgcgaagttt
     2881 gacacgctgt gcaaggcgat taccccagcc tccacgctag ccaccctcgt gcaaagtcca
     2941 cggctcgtct ctgagagaag agctttgctt ccgcagcccc atgcagtgaa tgaaacggcg
     3001 ggatgagagc tccgggcaag acgcggaagg tctcggggat gtggcccatc ctgcgggcga
     3061 aggctggcat gttctcacgg atgggttcta accaggaggc tgctcccaca tcccggggtc
     3121 ggagtactcc gcagagtgag tgggcatcat cagggttccc gactccgtaa cggcgtccat
     3181 cagagccttc accaccgcta cggaaccacc acagtcccca gtcctagctg gcgcaggtcc
     3241 gctgtcaggt tccggcgggt gcggggctcc ggcgtccggt ctatgagctc tttctctccc
     3301 taactcgaga gcgaggcgga tgctcaaccg tcatgagggt ttggggtcgt tcgaggcggg
     3361 tatgtacttt ttattagccc ggcggcccgc ggaggagtac taacccttcg tggacccccc
     3421 ctccaaagaa gccgccgggc cactctcatc tcgggccgga ggagatccta cccattgtct
     3481 tgcctttatt caccgaatct cgtagaagta gataaattct cggtaggccg cctgtacggg
     3541 gatgggctga ggtcgaagga ctcccacgcc ctcctagaaa gtgtaggatg tgggcagtag
     3601 ggaagcgaga acccgaacaa aggggcttga aggcgcatga gtaggggaac ggtaaagtgg
     3661 ttcagcgggg agaagggtta tggtttcata accccagacg agggtggcga ggatctcttc
     3721 gttcactact cggccataga ggggagtggg ttcaggagcc ttgaggaagg cgagagggta
     3781 agctacgagc cgaccagggg caggaaggga gagcaggcag agaacgtgcg cagggtcgag
     3841 tagggaggga ctctggtcct cctccgccgg ggcggtggtt tacccccatt ggcatctcgg
     3901 agaggacggt agtttggtcg atcccaccgc gggttggtga acggggcata tccggccacc
     3961 tgcggaggtt gctaaccctc tgtagaaacc gcaggcgcac cggcaaacct ccccgcggca
     4021 ggtccgccag agcgtccgtg tgtagcggtc gtggaattcc ggcgcgaaac ctcgctgcac
     4081 accggtcagc gagtccccgt cgaagtagag ggcgccacgt gcagtgggtc ggggacggcg
     4141 aaggtaccgc tgtgcgggtc cgtattgggt atgggttccc tcccggtagg accttcagac
     4201 gatgtgcgcg tcaacctggt gcgcccgcca ccccggcgac cgcgactcag gtgggcgcgt
     4261 gccgcgaggc gcccgtcatc ctgacggtgg acgctgcggc catgcacgcg gccggcgacc
     4321 tgtcgggctg gcccggctag ggcaggatct cgacgtaacc gtccgttccg tgcacgcgga
     4381 tccgctgccc atcccggatc agccgggtgg catgctccac ccccacgacg gccggcaagc
     4441 cgtactcccg tgcgatcacc gcgccatggg tcatcaggcc ccccacctcc gtcaccaggc
     4501 ccttgatcgc gacgaacagg ggcgtccagc tggggtccgt gtaggcggtg accaggat
//
LOCUS       DATAIR010000174         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_380129, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000174 DATAIR010000000
VERSION     DATAIR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380129"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VHM69_04175"
     CDS             <1..433
                     /locus_tag="VHM69_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739619.1"
                     /translation="TQVTFSATGVTFIGTSRSVTVTATYFGSTRAASLTVVGQEKVEK
                     ELAIAKEIETEFRARQSTGVERTEAPEERSGESTEEGEQSTAEEPEGSASGRAFVQPA
                     ERDLPGQEALDQPPAQKEEKAEKKEEKTRARRGRKKKRPEG"
     gene            393..1106
                     /locus_tag="VHM69_04180"
     CDS             393..1106
                     /locus_tag="VHM69_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739620.1"
                     /translation="MLVVVARRSDQKARDLAERWANYGGAVLTCDDLSVAGWRQLLGG
                     HETSMVVIDGQVVEARRIAGVLMLLPYVTEGELVRIAREDRRYVASEMTAFLHFWLSG
                     LPCPVLNRPTPVCLTGPGWRREQWVYVAARLGIPVEAVAWRLDRAAGVPEKKNSSAPG
                     ITVTMVGSRCLGSADGTLVSYVRRLSRAAGVEMLSVDFRETETGFRLYNARSSWADIS
                     SPEVADAILEYFLARSAGC"
     gene            1100..2107
                     /locus_tag="VHM69_04185"
     CDS             1100..2107
                     /locus_tag="VHM69_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739621.1"
                     /translation="MLILLWGLSTERPLAAVREELLSLDLPTITVDQHAVLDTEIRLN
                     VGRDLEGEIRVAKGRWIDLSEVSAVYLRPYDSRRVPAVRREGPDSTASRHAQAVDNVM
                     ASWSEMTSALVVNRLQAMATNDSKPYQLEQIRSSGFNVPETLVTTDPSAVQDFWEQHR
                     EVIYKSVSGTRSRVARLRLEHLERLGDVAFCPTQFQRYIPGTDHRVHVVGEEVFASEV
                     ISEADDYRYPGQHSVEIHARRLPRDVEERCRLLSVALQLPVAGVDLRRTPEGDWYCFE
                     VNPSPAFTHYASRTGQPIGRAVANLLATGLQTTMEPAETCPTREEKQLSRNAKLVNLP
                     V"
     gene            2426..>2921
                     /locus_tag="VHM69_04190"
     CDS             2426..>2921
                     /locus_tag="VHM69_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739622.1"
                     /translation="MPSVGQELLDTPFPEMVAKLAMGIAQAQQALDENSVNTAVILAD
                     TEIDVVLNITQVIAADGTVSFQQADPVSISLIQIGLIPTFYQFSEATIEVTMDIKTTT
                     SRETNVKVSASAKVGFAAWSASVTADVSHNRKFGKEVRGTSRLFTRLAPVPPPPRIAP
                     ELTVV"
BASE COUNT          629 a          857 c          928 g          507 t
ORIGIN      
        1 cacgcaggtt accttcagcg cgacgggcgt gacctttatt ggtacgagca ggagcgtaac
       61 cgtaaccgcc acctacttcg ggtcgacgcg ggcggccagt ctgaccgtcg tcgggcaaga
      121 gaaggtcgaa aaagagcttg cgatagcaaa ggagatcgaa acagagttcc gagcccgtca
      181 aagcaccggc gtagagcgca cagaggcgcc agaagagaga tctggcgaga gtactgagga
      241 aggcgagcaa tccactgctg aggagccaga aggttctgct tccgggcggg cgtttgtcca
      301 accggccgag cgcgaccttc ctggacagga ggccctggat cagcctccag cgcaaaagga
      361 ggagaaagcg gagaagaagg aggagaagac tcgtgctcgt cgtggtcgca agaagaagcg
      421 accagaaggc tagggacctg gccgagcgat gggcaaacta tggcggcgcc gtcctgacct
      481 gcgacgacct ctccgtggcg ggatggcgcc aactcctggg cggccacgaa acttcaatgg
      541 tggtgatcga cggacaggta gtcgaagccc gccggatcgc aggcgtcctc atgctactgc
      601 cgtacgtaac cgagggcgag ctggtcagga tcgccaggga ggatcgccgg tacgtggctt
      661 cggagatgac ggcattcctg cacttctggc tatccggcct gccgtgcccc gtgctgaacc
      721 ggccaacacc ggtctgcctg acgggaccag gctggcgccg tgagcaatgg gtgtacgtgg
      781 cggcccggct cggtatcccg gtagaggccg tggcgtggcg ccttgatcgt gcagcgggcg
      841 ttcctgagaa gaagaattct tccgcacccg gcattaccgt aaccatggtc ggctcgaggt
      901 gcctgggtag cgcggacggt acgcttgtct catacgtgcg acgcctgtcg agggcagccg
      961 gggtagagat gctgtctgtc gacttcaggg agacggagac cgggttcagg ctgtacaacg
     1021 cgaggtcctc ttgggcagac atctcctcgc ccgaagtcgc cgacgccata ctggagtact
     1081 ttcttgcgag gtcagccggg tgctaatcct cttatgggga ctgagcacgg agcgaccgct
     1141 ggcggcggtg cgcgaagaac tgctaagtct ggacttgccc acgatcacgg tcgaccaaca
     1201 cgccgtgctt gacaccgaga taaggctgaa cgtcgggcgc gacctagaag gagagatccg
     1261 ggtggccaaa ggcaggtgga tcgacctctc ggaagtgtcc gcggtgtacc tgcgccctta
     1321 cgactcccga cgtgtacccg cggtgaggcg agaggggcct gacagtacgg catcgcgcca
     1381 cgcgcaggcg gtggacaacg tgatggcttc ctggtcggag atgacctccg ccctggtggt
     1441 gaacaggctg caggcgatgg caaccaacga ctcgaagccg tatcagctag agcagatccg
     1501 aagctccggc ttcaacgtcc ccgagacact cgtcactacc gacccgagcg cggtgcagga
     1561 cttctgggag cagcacaggg aagtgattta caagtcggtg agcggcacgc ggagcagagt
     1621 cgcgcgcctg cgactcgaac acctcgaacg cctcggggac gtggcatttt gcccgaccca
     1681 gttccagcgg tacatcccgg gaacagacca tcgagttcat gtagtcggcg aggaggtttt
     1741 cgcaagtgag gtgatctcag aagcggacga ctaccggtat ccaggtcagc actcggtcga
     1801 gatccacgcc cgtcgtctcc ccagagacgt ggaggaacga tgcaggctgc tctctgtagc
     1861 cttgcaactt ccggtcgcgg gagtcgatct tcgtcggacg ccggagggag attggtactg
     1921 cttcgaggtc aacccgtctc ccgcttttac gcactacgcg agcaggacgg gccagcccat
     1981 cggtcgcgcc gtcgcgaacc ttcttgcgac cgggctacaa acgacgatgg agcccgctga
     2041 aacctgcccg acgagagagg agaaacaact atcgcgaaac gccaaactgg tgaacctacc
     2101 cgtgtaagtg ctctctgagg agccgtgggc gtgacgcggc tgtggcctcg gggaccccat
     2161 cttgcggcgc tagaagctac ggagtttgcc caatttgagc tattcaacca acactcatcg
     2221 caaattcatc cagaattcat cgctgtgcgc tatctttgca ggtcgcccga tgcatcacgc
     2281 cgccgcaggc gggcggagag gaggtccaat attcagggtg tcaaagaccg gtttgccgct
     2341 ttcggcaagc tcacgggact cgacccactc catcctcagt agaacagcca gatccctaca
     2401 aagtctttca aaggagtaga cactcgtgcc aagcgtagga caggaacttc ttgatacccc
     2461 gttcccagag atggtggcca agctcgccat gggcatagcc caggctcagc aagccctcga
     2521 tgagaactct gtaaacaccg cggtcatcct cgctgataca gagatcgatg tagtcttgaa
     2581 catcacccag gtgatagccg ctgacggcac ggtcagcttc cagcaggcag atcccgtctc
     2641 catctccctc atccagatag gtctgatccc caccttctac cagttctcgg aagcgaccat
     2701 cgaagtaacg atggacatca agacgacgac gagcagggag acgaatgtaa aggttagtgc
     2761 ctccgccaag gtaggattcg ccgcgtggag cgcatcagtt acggctgacg tgtcccacaa
     2821 ccgcaagttc ggcaaggaag tgagaggaac gagccggctg ttcacgcgcc ttgcgccggt
     2881 accgccgcca cccaggatag cgccggagct tactgtggtg g
//
LOCUS       DATAIR010000175         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_103288, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000175 DATAIR010000000
VERSION     DATAIR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103288"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..550)
                     /locus_tag="VHM69_04195"
     CDS             complement(137..550)
                     /locus_tag="VHM69_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2739623.1"
                     /translation="MRTSTNYGIQREYDSRLETPALGVMSIEGERMWSTLAHLSTFLN
                     LFTGFLGPVVAFVIWLVYKDDSPTVAAHALRSVFYQVAWLSAIFIGWTITVALMVVLI
                     GFLLVPVMALVTLAPFVQASYEAYRAYMGGGRRYI"
     gene            752..2089
                     /locus_tag="VHM69_04200"
     CDS             752..2089
                     /locus_tag="VHM69_04200"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2739624.1"
                     /translation="MTEEAQGTGSGQGSTATGAESLTVTDNRTGKNYDVKISDGTVRA
                     MDFRQIKVDEDDFGLMTYDPGYSNTASCRSAITYIDGEKGILEHRGIPIEQLCEHSTY
                     LEVAYLLNYGELPTEAELDRWIYEITHHTYVHENIKNFMNGFRHDANPMGMLQASVGA
                     LSTFYPEAQDIDDEYQRHVSAVRLIAKMPTLAAFAYRHSLGLPYVYPDNDLSYAGNFL
                     SMLFKMAEPKYEPDPRLEKALDVLFILHADHEQNCSAAAVRVVGSSRVDPFSAITAGC
                     AGLYGPLHGGANVAVLRMLKRVGSVDNIPAFLEGVKQGKERLMGFGHRVYKNYDPRAR
                     IIRGHVDEVLEATNKESPLLDIAIELEKHALDDDYFTSRKLYPNVDFYSGLIYEALGI
                     PTDAFTVMFAIPRTSGWMAQWMEMMVDPELKIARPRQIYTGPRDIDYVPIDER"
     gene            2362..2682
                     /locus_tag="VHM69_04205"
     CDS             2362..2682
                     /locus_tag="VHM69_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX2739625.1"
                     /translation="MDNKKLAASAVAVVLALGAGGAAYAVGQGEKVTGPDAEKAKSAA
                     LRAVGGGTVTEVERSDGDGAFEVEVERSDGSQVEVRLDRTLDVVAKAAGDDGPNDDEG
                     PNED"
     gene            2752..>3106
                     /locus_tag="VHM69_04210"
     CDS             2752..>3106
                     /locus_tag="VHM69_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739626.1"
                     /translation="MGGKRMRPMMRVVAMIVVLAGLSVIGACGADTATKKDQSSRDAA
                     AGSKTAEGESGSKSIPQPVMLEDLATAPEGARVDISMPVFSNPTEITNPLFPVSKQES
                     VLMVGRVDDKPFRTEV"
BASE COUNT          695 a          943 c          937 g          531 t
ORIGIN      
        1 cgctcgagga ggaagtttcc aggaactaca gctgaactcc tggcgatggg accgctgaaa
       61 tgtagaccga cgtcacccgg cttctgaacg gacgactagc tcaatcgggc tcacctcagg
      121 tatgcgtatc gacactttag atgtagcggc ggcccccgcc catgtaggcc ctgtaagctt
      181 cgtaagaggc ctggacgaac ggtgccagcg tcaccagagc catgacgggc acgagcagga
      241 acccgatcaa gacgaccatc aaagccaccg taatagtcca gccgatgaag atggcggaaa
      301 gccaggccac ctggtagaag accgacctca gcgcgtgcgc ggccaccgtg ggcgagtcgt
      361 ccttgtagac cagccagatc acgaacgcaa caacaggccc aagaaacccc gtgaagaggt
      421 tcaggaaagt gctcaaatgt gccagcgtcg accacatgcg ctcgccctcg atgctcataa
      481 caccaagggc cggcgtttcg agccgcgaat cgtactcgcg ctgtatcccg tagttcgtgc
      541 tagtcctcat gaacgctcct ttctcaccgc ggataaccgc gtatacgctg gcgccggaag
      601 aaggtttcca acccaccatc gtccttcgtc gcaaggctac tttccgtttt actcctggta
      661 ggcgataata cgcgcacctc aggaggcggt tcgacgtaat cacatatgtg tcacgagcat
      721 atataatccg tgcaagagaa aggggcgaag gatgaccgaa gaggcgcaag gcacggggag
      781 tggccagggt tcgacagcca cgggggctga gagcctcacg gtaacggaca accggactgg
      841 caagaactac gacgtcaaga tatcggacgg cacggtccga gcgatggact tcaggcagat
      901 caaggtcgac gaggacgact tcgggttgat gacctacgac cccggttact ccaacacggc
      961 gagttgtcgt agcgccataa cgtacataga tggggagaag ggaatcctcg agcacagggg
     1021 catccccatc gagcagcttt gcgagcattc cacgtacctc gaggtcgcct atctcttgaa
     1081 ctacggcgag ctcccgaccg aggcagagct tgaccgctgg atctatgaga tcacccacca
     1141 cacctacgtc cacgagaaca tcaagaactt catgaacggc ttcaggcacg atgccaaccc
     1201 gatggggatg ctgcaggctt cggtaggcgc gctctccacc ttctaccctg aggcccagga
     1261 catagacgac gagtaccagc gtcacgtctc cgctgtgcga ctcatcgcca agatgccgac
     1321 cctcgccgcc ttcgcctaca ggcacagcct cgggttgccg tacgtctacc cggataatga
     1381 cctctcatac gcgggcaact tcctttccat gctcttcaag atggccgaac ccaagtacga
     1441 gccggacccg cgtctcgaga aggctctcga cgtactgttc atcctgcacg ccgaccacga
     1501 gcagaactgt tccgctgcgg ccgtaagggt cgttggatct tcgagggtgg atcccttctc
     1561 agccatcacc gccggttgcg ccgggctcta cggaccgttg cacggcgggg ccaacgtcgc
     1621 cgtcctgagg atgctaaagc gggtagggag cgtcgataac atcccggcct tcctggaggg
     1681 tgtcaagcag ggcaaggagc gtctcatggg cttcggccac agggtgtaca agaactacga
     1741 cccgcgagcc aggatcatcc gcggccacgt ggacgaggtg ctcgaggcca ccaacaaaga
     1801 gagcccgctg ctcgacatcg ctatcgagct cgaaaagcac gccctcgacg acgactactt
     1861 cacgagccgc aagctctatc ccaacgtgga cttctactca ggtctcatct acgaggccct
     1921 cgggataccg acagacgcat tcaccgtcat gttcgccata ccccgcacct cgggttggat
     1981 ggctcagtgg atggagatga tggtagaccc cgagctgaag atcgcccgtc cgcgccagat
     2041 ctacacgggt ccccgcgaca tcgactacgt acccatcgac gaaagatagg tagcgtccgc
     2101 actcagccgg gcttcactgt caagcacctc tgcggaggcc gaatcagcct ccgcagaggt
     2161 ccgcgccata taggtcgccg cgggtacgga gcaggccgtt gctctggatc gggcgctctt
     2221 tcggacatcg tgcacgacga ataaagacat acagcctcct actctagaaa atctcatgct
     2281 gctctaagct gcctctaaag ccactctcag ggttcggcgg aatactccat ccagataagc
     2341 ccccgacgag tggaggagag tatggacaac aaaaagctcg ctgcgagcgc cgttgcggtc
     2401 gtgctggctc tcggcgcggg cggagccgcc tacgccgtcg gccaggggga gaaggtcacc
     2461 gggcccgacg ccgagaaagc caagagcgcc gcactccggg cggttggtgg cggaaccgtc
     2521 accgaagtcg aacggtcgga cggggatggc gccttcgagg tcgaggtcga gcggtcggac
     2581 ggcagccagg tcgaggtgcg cctggatcgg acactcgacg ttgtcgccaa ggcagcgggc
     2641 gacgatggtc ccaacgacga cgaaggtccg aacgaggatt gatcccggag cgaattagtt
     2701 cctgacgcgt cgctcgtgga caagcgcgga cgcggctata gaggaggagt catgggtggc
     2761 aagcgcatga gaccgatgat gcgcgtcgtt gcgatgatag ttgtattggc cggcctgtcg
     2821 gtgatcgggg cgtgtggggc cgatacggcg accaaaaagg atcagagttc ccgcgatgcg
     2881 gcagccggct ccaagacggc agaaggggag agcggatcaa agtccattcc ccagccagtc
     2941 atgctagagg accttgcgac ggcgccggag ggagcgcgag tagatatcag catgccagtc
     3001 ttctcgaacc ctaccgagat caccaacccg ctatttcctg tctcgaagca ggagtcggtc
     3061 cttatggtcg gccgggtcga tgacaagccg ttccggaccg aggtca
//
LOCUS       DATAIR010000176         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_380233, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000176 DATAIR010000000
VERSION     DATAIR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380233"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VHM69_04215"
     CDS             complement(<1..273)
                     /locus_tag="VHM69_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2739627.1"
                     /translation="MDDSAQQRLFAETPGSIEPPERGLYLGTSGWSYADWEGTLYPEA
                     LPSGSRLAEYVKRFATVEIDSTFYGTPRRSTVQNWREIAPDGFLFAA"
     gene            382..1320
                     /locus_tag="VHM69_04220"
     CDS             382..1320
                     /locus_tag="VHM69_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX2739628.1"
                     /translation="MKVFYSDRFVLPLPEGHRFPMVKYSMLRERVADDGICGPGDLRI
                     PRAVTDEEILRAHDPHYLKRVVSGTLTEKEMRRIGFPWSEKMVERSRRASGGTLGACL
                     AALDDGFSANLAGGTHHAFSDRGEGYCVFNDSAIAARAVQAGGLVERVVVIDTDVHQG
                     NGTAAILRPDPTVFTFSIHGAKNFPFRKEESDLDAPLPDGADDTEFLTSLEEGLEAAL
                     DAADAGLAIYLAGADPYEGDRLGRLSVTKAGLAERDRIVLETCRERGIPVAITMAGGY
                     ARNVVDTVDVHFESIKRAAQLLERNPSAENPGRAHL"
     gene            1320..2327
                     /locus_tag="VHM69_04225"
     CDS             1320..2327
                     /locus_tag="VHM69_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2739629.1"
                     /translation="MRDIPNRRSCIVVGAGISGLLSASALQSKGWNVTVLEKRPGVGG
                     RMATVQLGEGNFDHGAQFFTVRGERFAGLVKGWLAAGVAVEWSRGFADADGRPNEDGH
                     PRYRGAEGMTSIPRFISRGLDVRTGESVVTVDAEDDGWEVACTSGMRVAGDALVITAP
                     VPQALRLISSGNHVLPDEARRRLEAISYDPCLALMVSLDGPSGVPEPGGMQIKGEPLD
                     WIADNHRKGISEAPGITIHAGPGWSRTHFDDEEVGIRHNLISLAGDHLGTDLAPRVVE
                     TRLTRWRHSWVTSPHPDLCLAASEDPPLILCGDAFGQPKVEGAALSGLAAADHLLAYK
                     A"
     gene            2549..>2766
                     /locus_tag="VHM69_04230"
     CDS             2549..>2766
                     /locus_tag="VHM69_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739630.1"
                     /translation="MGNRRVALKPHASKIRRWVEEGRGDKWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHPAVLDETEDG"
BASE COUNT          522 a          891 c          872 g          481 t
ORIGIN      
        1 ggccgcgaag aggaagccat cgggtgcaat ttcacgccaa ttctgtacgg tcgagcggcg
       61 cggggtgccg tagaaggtcg agtctatctc gacggtcgcg aatcgcttga cgtactcggc
      121 gagccgcgac cctgaaggga gcgcttcggg atagagtgtt ccctcccagt ccgcatacga
      181 ccagcccgag gtcccgaggt acaggcctct ttcaggcggc tcgatgctgc ctggtgtctc
      241 cgcgaagagc ctttgttgcg cggaatcgtc cattcgctta caagctacca tactgtccag
      301 ggaggctctt atgaacgttg tcgctgttcg ccctcgccgc gtgtactaca ttattgcgat
      361 ggacgagaga cgccttatat catgaaggtc ttctacagcg accgcttcgt gctcccgttg
      421 ccggagggcc accgcttccc gatggtcaaa tactcgatgc tgcgcgagcg tgtggccgac
      481 gatggtatct gcgggccggg agatctccgg attccgcgcg ctgtgacgga cgaggagatc
      541 ctgcgcgccc atgatccaca ctacctgaaa cgcgtcgtct cgggcacgct gacggagaag
      601 gagatgcggc gcatcggttt cccatggtcg gagaaaatgg ttgagcgctc ccgccgcgcc
      661 tcaggcggca ctttgggtgc atgcctcgcc gccctcgatg acgggttctc cgcgaacctc
      721 gcaggcggga cgcaccacgc gttttccgac cggggagagg ggtactgcgt cttcaacgac
      781 tcggccatcg cggcccgagc cgtccaggcc ggaggactcg tcgaacgggt cgtcgtcatc
      841 gacaccgacg tccatcaggg gaacgggacg gcggcgatcc tgcgcccaga ccccactgtc
      901 ttcactttct cgatccacgg cgcaaagaac ttccccttcc gcaaggagga gagcgacctc
      961 gacgcgcctc tcccagacgg cgcagacgat acagagttcc tcaccagcct cgaagaggga
     1021 cttgaggcgg cgctggacgc cgctgacgcg gggctggcga tctacctcgc cggtgcggac
     1081 ccttatgaag gcgatcgcct cggccggctc tctgtcacga aggcaggcct cgccgagcgc
     1141 gaccgaatcg tcctcgaaac gtgccgtgaa cgcggcatcc ccgtcgcgat aacgatggcc
     1201 gggggctacg cccgcaacgt cgtggacacg gtggacgtgc acttcgagag catcaaacgc
     1261 gccgcacagc ttctggagag aaacccctca gcggaaaacc ccggaagggc gcatctataa
     1321 tgagagacat ccctaatcgg agatcctgca tcgtcgtggg agcgggtatc tccggcctgc
     1381 tctccgccag cgcgttgcag agtaaaggct ggaacgtaac ggtcctcgag aaacgtcccg
     1441 gcgtcggcgg caggatggct actgtacagc taggcgaagg caacttcgac cacggcgccc
     1501 agttcttcac agtgagggga gaacgcttcg ccggccttgt gaaaggttgg ctcgccgccg
     1561 gcgtagccgt ggagtggtcg cgaggcttcg ccgacgcgga cggtcggccc aacgaagatg
     1621 gccatccacg ctacaggggc gccgagggca tgacctccat ccccaggttc atctcccgcg
     1681 gcctcgacgt ccgtacagga gaatcggttg tgacggtcga tgccgaagac gatggctggg
     1741 aggtcgcctg cacctccggc atgcgggttg cgggcgatgc tctcgtgatc accgctcccg
     1801 ttcctcaggc tctgcgcctc atctcgtcgg gaaatcatgt cctgcctgac gaagccaggc
     1861 gacggctcga agccatctcc tacgaccctt gcctcgcgct catggtttcg ctcgacggtc
     1921 cgagtggcgt acccgagccg ggagggatgc agatcaaagg cgagccgctt gattggatcg
     1981 cagacaacca ccggaaaggt atctccgaag cccctggcat cactatccac gccggaccag
     2041 ggtggagccg cacgcacttc gatgacgaag aggtggggat caggcacaac ctgatctcgc
     2101 tcgccggcga ccacctcggc accgacctcg cgccaagggt cgttgagacc cgcctcacca
     2161 ggtggcgcca tagttgggtc acgagccccc atcccgacct atgcctggca gcctccgaag
     2221 acccgcccct gatcctctgt ggcgacgcct tcggacagcc aaaggtcgaa ggcgcagccc
     2281 tctcgggcct cgccgccgcc gaccacctcc tggcctacaa agcctgagcc accgtcgacg
     2341 cgagccactc tagcctgcta tcaggagcct cttcgcactg tttccgctct cgttcccatc
     2401 gtgcgggtct tcgtcgcggg ccaggtgagc cagtcggttc cggtacattg gcgccccgcc
     2461 cgacaagtgt gaagatgtat ctggcgtcgg gtaacaaaaa gtggtacata taacctagaa
     2521 aactagtcgg gcgaaggaga agggggccgt gggaaacaga agggtggcgc taaagccaca
     2581 tgcgtccaag atccgtcgct gggtcgaaga gggtcggggt gacaaatgga tcgcgcagga
     2641 gttgaacacc acgccgtcga gcgtgcagag cttccggtcg cggaactcga tctaccgccg
     2701 cgaccccgtc aggcgcggcc agctctcgga gcatcccgcg gtgctcgacg agactgaaga
     2761 tggcat
//
LOCUS       DATAIR010000177         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_103471, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000177 DATAIR010000000
VERSION     DATAIR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103471"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VHM69_04235"
     CDS             <1..395
                     /locus_tag="VHM69_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2739631.1"
                     /translation="GHIHDHAKETYPEECSGVMVGMDVGEMKIVVDVWRAENTFEADE
                     RGHRFLIDPKEYIAFEKRAGERDMDILGVYHSHPDHPAEPSDYDREHAWPGWSYVIAS
                     VSENGVEDMRSWLLKDDRSGYEDEPIVG"
     gene            424..717
                     /locus_tag="VHM69_04240"
     CDS             424..717
                     /locus_tag="VHM69_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX2739632.1"
                     /translation="MPKVRIPTPLRQYTEGNSEVEVGGDTAGAALDDLTGQHPALRQH
                     LYTGDGRLRSFVNVYKGDEDIRYLDGSDTEVADGDELSIIPSIAGGAAGRRCR"
     gene            781..>1854
                     /gene="moeB"
                     /locus_tag="VHM69_04245"
     CDS             781..>1854
                     /gene="moeB"
                     /locus_tag="VHM69_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX2739633.1"
                     /translation="MKTRQPLVESPNGAVELSNDEIARYSRHLIMPEVALGGQKQLKQ
                     AKVLTVGTGGLGSPLALYLAAAGVGTIGIVDFDVVDESNLQRQILHGTSDVGRPKVES
                     ARDRIKDINPNVNVIVHEEALTSENALDILKDYDVIVDGTDNFPTRYLVNDACVILGK
                     PNVYGSIFRFEGQASVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQ
                     ATETVKLILGIGEPLIGRLLLYDALGMRFREMKLRKDPNCPVCGENPTVTELIDYQEF
                     CGIPQANAQAQADEVPEITVQELEGRLRNGSKVSVLDVREPHEYEVANIGARLIPLAE
                     LPERLVELDRDETLAVHCKSGGRS"
BASE COUNT          393 a          579 c          584 g          298 t
ORIGIN      
        1 tcggccacat ccacgaccat gcgaaagaga cgtatcccga ggagtgctcg ggggtaatgg
       61 tgggcatgga cgtcggggag atgaagatcg tggtcgacgt gtggcgcgcg gagaatactt
      121 tcgaggcgga cgagcgcggt caccgttttc tcatagatcc gaaagagtac atagctttcg
      181 agaagcgggc tggcgagcgg gacatggaca tccttggcgt gtaccattcg cacccggacc
      241 atcctgcgga gccgtctgac tacgaccgcg agcacgcgtg gcctggctgg tcgtatgtga
      301 tcgcctccgt ctcagagaat ggggtcgagg atatgcgatc gtggctgctc aaagacgacc
      361 gctcgggcta cgaagacgag cccatcgtag gttagaaact cagatacacg gaaggaaaat
      421 ctaatgccga aagtcagaat tccaacacct ttaagacagt acacagaggg caactccgag
      481 gtcgaagtcg gcggcgacac cgccggggcg gccctggatg acctcacagg acaacacccc
      541 gccctgcgac aacacctcta tacgggcgac gggaggctcc gctcctttgt taacgtgtac
      601 aagggcgacg aggacatccg ctacctcgat ggctctgaca ccgaggtagc ggatggcgac
      661 gaactctcca tcatcccctc catcgccggt ggcgccgccg gaaggcgatg taggtaattt
      721 cttagataca gatttttctg ggcaatcagt agtttcagaa ccggaaggac attgcatacc
      781 atgaagacgc gacagccatt agtagagtct cccaatgggg cggtcgagct ctcaaacgac
      841 gagatcgcac gctacagccg ccacctcatc atgccggagg tggcgctcgg cgggcagaag
      901 caactcaagc aggcgaaggt gctcaccgtc ggcaccggcg gcctcgggag cccgctcgcg
      961 ctctatcttg cggcggccgg ggtcgggacc atcgggatcg ttgacttcga tgtggtcgac
     1021 gaatccaact tgcagcgcca gatcctgcac ggaacctccg acgtgggccg cccgaaggtc
     1081 gagagcgcga gggatcgcat caaggacatc aacccgaacg tgaacgtcat agtgcacgag
     1141 gaggcgctca cgagcgagaa cgcgctcgac atcctcaagg attacgatgt gatcgtggac
     1201 ggcacggaca acttcccgac gcgctacctg gtcaacgacg cctgcgtaat cctcggcaag
     1261 ccgaacgtat acggctccat cttccgtttc gaaggacagg cgagcgtttt ctacgccgag
     1321 gaaggcccat gctaccgctg cctctacccc gagccgccgc cgccgggcct cgtcccgagc
     1381 tgcgccgagg gcggggtcct cgggatcctt ccgggcgcga tcggcacgat ccaggctaca
     1441 gagaccgtca agctgatcct cgggatcggg gagcccctga tcggacgcct cctgctctac
     1501 gacgccctcg ggatgcgctt ccgcgagatg aagctgcgca aggacccgaa ctgcccggtc
     1561 tgcggcgaga acccgaccgt caccgagctc atcgactacc aggagttctg cggcatcccc
     1621 caggccaacg cccaggcgca ggccgacgaa gtgccggaga tcacggtcca ggagctcgag
     1681 ggacgcctgc ggaacggctc gaaggtctcc gtcctcgacg tgcgcgagcc gcacgagtac
     1741 gaggtagcga acatcggcgc gaggctcatc cccctcgccg agctgcctga gcgcctggtc
     1801 gagctcgacc gggacgagac cctggccgtc cactgcaaga gcggcggccg cagc
//
LOCUS       DATAIR010000178         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_103533, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000178 DATAIR010000000
VERSION     DATAIR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103533"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VHM69_04250"
     CDS             complement(<1..213)
                     /locus_tag="VHM69_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739634.1"
                     /translation="MSAGYLAVDLGAESGRVVLGRFDGGRVSLEEVHRFPNVPVRLPD
                     GLHWDVLRIFREIKDGLAKAGRELQIE"
     gene            complement(210..>2938)
                     /locus_tag="VHM69_04255"
     CDS             complement(210..>2938)
                     /locus_tag="VHM69_04255"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HEX2739635.1"
                     /translation="MSMIAAGIQPVRLRCEYREDPLGIDERVPRLSWALESEARNQRQ
                     SAYRILVAANEEDLEAEQNLLWDSGRVESGRTVGVEYEGEALRSGSRCVWKIRVWDGA
                     DGASPFSELAVLEMGLLERSDWNGTWISAGKDPDKDFDPPSGDEYDDLANGLTPSPYL
                     RMEFSLDSSVRTARLYATARGVYELYLNGKRVGADVLAPGWTDYDQRVQYQSYDVTPL
                     LADGPNALGAVLGDGWFAGFVGFDPKHRGALYGPRPQLLAQLNLEYEDGTKETIATDG
                     SWSCSTGPILFSDLLMGESYDARKEMPGWNESGFDDSAWFGVEAEEIGDTNLVAQPDE
                     GIRVTEEIEAKTVTEPESGVYVFDLGRNMVGWVRLKVEGEAGTEVTLRHTEALNPDGS
                     VYTTNLRSARATDHYVLAGDGEEVYEPRFTFHGFCYVEVTGYPGEPPLGAVTGRVVHS
                     ATPPTGSFECSSEMVDKLQENILWGQRGNFLSVPTDCPQRDERLGWMGDAQVFVRTAA
                     FNMDVAAFFEKWMVDVEDAQSPEGAFPDVAPLLRGSGLIDLRWGAPAWGDAGVIVPWT
                     IYRTYDDTRIVERHYDAMTRWMEYLYEGNPNFLRKNRMGNNYGDWLSPRGDHTPKHLL
                     ATAYWAYDAKLMAEMAGVTGRHEDAKKYRDLHERIRAAFNDAYVAPDGRIEGDTQTCY
                     LLALHMGLLPEELRATAAAHLVRAIEREDWHLSTGFAGVGYLCPVLTETGHTDVAHRL
                     LNNETYPSWGYTIKNGATTIWERWDGWTEEDGFQSPNMNSFNHYSLGSVGEWLYRYVA
                     GIDLGTPGYGRIVIRPRPGGGLTHAKAEYDSVRGRISSAWKIEDDRFELEVIIPPNTT
                     ATVHVPVADGVSEGGKPVEEAEAVEFISAGAGETVLAVGSGRYEFAGMVVRE"
BASE COUNT          498 a          976 c          902 g          562 t
ORIGIN      
        1 ctcgatctgt agctcccgtc cggctttcgc cagaccgtct ttgatctcac ggaagatccg
       61 cagaacatcc cagtgcaacc cgtcaggtag acggaccggc acgttgggga agcggtgaac
      121 ctcctcgagc gagacgcgcc cgccgtcgaa gcgccccaga acgacgcgtc cgctctcggc
      181 cccgaggtca accgcgaggt accctgcgct cattcccgta ccaccatgcc agcgaactcg
      241 taacgtcccg acccgacggc aagaacggtc tcccccgctc cggcgctgat gaactcgacg
      301 gcctcagcct cttcgaccgg cttgccgccc tcggagacac cgtccgcgac cggcacatgg
      361 acagtggcgg tggtgttcgg agggatgatg acctctaact cgaaacggtc gtcctcgatc
      421 ttccaggcgc tggagatcct gcctcgtaca gaatcgtact cggccttagc atgcgtcaga
      481 ccgccgccgg gacgcgggcg gataacgatg cggccatagc ctggcgtccc aaggtctatc
      541 cccgccacgt aacggtagag ccactcgccc accgaaccca gagagtagtg gttgaaggag
      601 ttcatgttcg gcgactggaa gccgtcctcc tctgtccagc cgtcccagcg ctcccagatc
      661 gtggtcgccc cgttctttat ggtgtatccc cacgaggggt aggtttcgtt attgagcaaa
      721 cgatgggcga cgtccgtgtg gccggtttcg gtcagaacgg ggcacaggta gccaaccccc
      781 gcgaatccgg tcgagaggtg ccagtcctcc ctctctatgg ccctgaccag gtgagccgcg
      841 gcggtggccc ttagctcctc aggcaagagg cccatgtgca gggcgaggag gtagcaggtc
      901 tgggtgtcgc cctctatccg gccatcgggc gcgacgtagg cgtcgttgaa cgcggctctg
      961 atgcgttcgt ggaggtccct gtatttcttt gcgtcctcgt ggcggcccgt gaccccggcc
     1021 atctcggcca tgagcttcgc gtcgtatgcc cagtaggccg tggcgagaag gtgtttgggt
     1081 gtgtgatccc ccctcggtga gagccagtcg ccgtagttgt tccccatcct gttcttgcga
     1141 aggaagttgg ggttgccttc gtagagatat tccatccagc gggtcatggc gtcgtagtga
     1201 cgctcgacga tgcgcgtgtc gtcgtaggta cggtagatcg tccacggcac gataacgccc
     1261 gcgtcacccc aggcgggcgc gccccacctc agatcgatga ggccagagcc cctgagcaaa
     1321 ggggccacgt cggggaaagc accctccggg gactgggcgt cttcgacgtc gaccatccac
     1381 ttctcgaaga aggctgcgac gtccatgttg aaggccgctg tgcggacgaa gacctgcgca
     1441 tcccccatcc agccgaggcg ctcgtcgcgc tgcgggcagt cggtcggcac gctgaggaag
     1501 ttgccgcgct ggccccagag gatgttctcc tgcagcttgt cgaccatctc actagaacac
     1561 tcgaaggacc ctgtaggcgg cgtggccgag tggacgacac ggcctgtgac ggccccgaga
     1621 ggaggctcgc caggatagcc ggtgacctcg acgtagcaga agccgtggaa cgtgaagcgt
     1681 ggctcgtaga cctcctcccc gtcgccggcc agcacgtagt gatccgtggc cctggcggac
     1741 cggaggttcg tggtatagac cgagccgtcg gggttcaggg cctcggtgtg gcgcagggtc
     1801 acctccgttc cggcctcgcc ctcgaccttc agacgtaccc acccgaccat gttcctacca
     1861 aggtcgaaga cgtagactcc gctctctggc tcggtgacgg tcttcgcctc tatctcttct
     1921 gtaaccctga tgccctcgtc aggctgggca acgaggttgg tgtcccctat ctcctcggct
     1981 tccaccccga accacgccga atcgtcgaag cctgattcgt tccagccagg catctccttg
     2041 cgggcgtcgt acgactcgcc catcaggagg tcggagaaga ggatcgggcc cgtcgagcaa
     2101 ctccacgaac cgtcggtcgc gatagtctcc ttcgtgccgt cctcgtactc gaggttcagc
     2161 tgtgcgagga gctgcgggcg gggaccatag agggctcccc tgtgctttgg gtcgaacccg
     2221 acaaaccctg cgaaccagcc gtcgccgagt acggcgccaa gggcgttggg gccatcagct
     2281 aatagcggcg tgacgtcgta gctctggtac tggacgcgct ggtcgtagtc cgtccagccg
     2341 ggggcgagta cgtctgcccc cacccttttc ccgttcaaat agagctcgta gacgccgcgg
     2401 gccgtcgcgt agaggcgcgc cgtgcggact gaactgtcca gcgaaaactc catgcggagg
     2461 tacgggctcg gggtaagccc gttggccagg tcgtcgtact cgtcccctga aggggggtcg
     2521 aagtctttgt cggggtcttt gccagcggag atccaggttc cattccagtc tgatctctcg
     2581 agcaaaccca tctctaagac ggcgagttcg ctgaaaggtg aggcgccatc tgccccgtcc
     2641 cagacacgta tcttccacac acaccgcgag ccagaccgaa gcgcctcgcc ctcgtactcg
     2701 acgccgacgg tacgccccga ctcgaccctg ccgctgtccc acaggaggtt ctgctccgct
     2761 tcgagatcct cctcattggc cgctaccagg atacggtagg ctgactgcct ctggttccgt
     2821 gcttccgact ccagcgccca gctcaacctc ggaaccctct cgtcgatgcc gagtgggtct
     2881 tcgcggtact cgcacctcaa ccggactggt tggatcccgg ccgcgatcat actcatct
//
LOCUS       DATAIR010000179         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_288498, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000179 DATAIR010000000
VERSION     DATAIR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288498"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHM69_04260"
     CDS             <1..723
                     /locus_tag="VHM69_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2739636.1"
                     /translation="PFASSFAAFFSRAYDFIRMGAISGANIRLSGSHAGVSIGEDGPS
                     QMALEDIAMMRAVHGSTVLQPCDANQTAKLVAEMADTDGIVFLRTLRPKTPVIYAPTD
                     DFPIGGSKIVRSSDDDAVTVVATGITVHEAVKAADELKESGVAVRIIDAYSIKPIDGE
                     TLRAAARATDGKVVAVEDHWPEGGLGEAVLSALSEETNALHFEHLAVGIMPGSGKSDE
                     LMDAAGISANHIADAARKLANG"
     gene            933..3854
                     /locus_tag="VHM69_04265"
     CDS             933..3854
                     /locus_tag="VHM69_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2739637.1"
                     /translation="MVRTRILLLGAPHVEHDGVPVEVDTRKAIALVAYLAVTQRGHTR
                     DALAGLLWPEYNQSRARAALRRTLSSLGKARAEGWLRVDREGVDLADDAGLWVDVDRF
                     RGLLAECRTHGHADPEVCPECLPPLTEAATLYRDDFMAGFGLRDSVAFDDWQFFQSES
                     LRRELAGALEKLARGRGALGEWESAVAHARRWLALDTLHEPAHRLLMALYAWSDQRAA
                     ALRQYRECVRILGQELGVAPLEETTLLYHAIQENDLPPQPVLSEHHRPAPREAQSVSL
                     VETSTVATYSPDHPLVGRATEWAALLGSYGAVGNKGRVVILEGEAGIGKTRLAEEFVA
                     HLRTEGATAVAARCYAGEKNLAYGPFVEGLSSTLGGENAGRLKGLPAGSLQEAARLLP
                     DLASLSPDSPPAPPLDTPGARSRFFGELVRVLLAVLDGPPPGILFLDDLHWADDASLS
                     LLTYLVRRLEDKPLLVLLTWRAEEVREGHRLRGLLTEARRSGVATALTLERLSPASVE
                     ELVGSAVPGAWTLGSRLSDETEGLPLFLTEYMAAVEKGELSAGDDAWALPGGVQDLLR
                     TRLLTVGETAGQVLAAAAVVGRSFDFDTVRAASGRGEEETLTALEELSSRGLIREVGS
                     AAGGAPAYDFDHDKLRTLVYEETSLARRRLLHRRAAAALAGQARGREADSLTGQIARH
                     HRLAGQDEEAAQYYRLAGDYARSLHANSDALAHYEEALALGYPDTTTLHEAIGDLRTR
                     AGEYGAALASYEAAASSSDAGDLSILEHKIGNVHARRGEWDLARSHYQLALEAFGEQE
                     GGAELARLYADWSLLSHGQDERDEATEFASRALELAESAGDTRALAQAHNMLGILAGR
                     SGDQDAALRHLQVSLDLAETLGDSDAKVAALNNLALAREHGGEPGEAMKLAETALALC
                     VASGDRHREAALHNNLADLLHAEGREQESMAHLKRAVELFAEIGEEDELQPEIWKLVE
                     W"
     gene            complement(3873..4349)
                     /locus_tag="VHM69_04270"
     CDS             complement(3873..4349)
                     /locus_tag="VHM69_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739638.1"
                     /translation="MWDEINEYLPDFRSAVLTGVDIEGYPFSVRCWPYPDPAGAEVLR
                     VQLPADTPLQPGSAGLLCHKHDENLWHLKSFLVRGVLGRDERGWKLEPLRFIPGAGIG
                     GLPAMVRFFTSSRRNAARYLDKRGIARPRIPWDEINAVKAQAFAPSDNGGEAGRRG"
     gene            complement(4339..>4767)
                     /locus_tag="VHM69_04275"
     CDS             complement(4339..>4767)
                     /locus_tag="VHM69_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2739639.1"
                     /translation="ARDGTPITWPTLPFWKPDEGRFMITTSISLSQKVSNVRRNPRVS
                     LLFSNPTASGLKDPPAVLVQADAEAADEIMTSVEGFEDELRMVYQRQPSSRVYSSNPL
                     MRYFMDWYYMRLVIHVTPRRILWWPKCNFSQSPFEVDHVG"
BASE COUNT          853 a         1581 c         1581 g          752 t
ORIGIN      
        1 ccgttcgcat cgagcttcgc cgctttcttc tcccgcgcct acgacttcat caggatgggc
       61 gctatctcgg gcgccaacat ccgcctctca ggctcgcacg caggcgtctc catcggtgag
      121 gacggtccgt cgcagatggc cctcgaggac atcgcgatga tgcgggccgt gcacggctcg
      181 accgtactcc agccctgcga cgccaaccag accgcaaagc tcgtcgccga gatggcggac
      241 actgatggca tcgtcttcct gaggaccctg cgcccgaaga cgcctgtgat ctacgccccc
      301 acagacgact tccccatcgg tggcagcaag atcgtacgct cctccgacga cgacgcggtg
      361 acggttgtcg caacgggcat cacggttcac gaagccgtaa aggccgcgga cgagttgaaa
      421 gagagcggcg tagcggtgcg catcatagac gcctactcca taaagccgat agacggcgag
      481 acgttgcgcg ccgccgcacg cgcaacggac ggcaaggtcg tggcggtcga agaccactgg
      541 cccgagggcg gcctgggtga ggccgtcctg tccgcgctct ccgaggagac caatgccctc
      601 cacttcgagc acctcgccgt tggcataatg ccaggctcgg gtaagtccga tgaactgatg
      661 gacgcggccg gcattagcgc gaaccacata gccgacgccg ctcgcaagct ggcaaacggg
      721 tagagcgtag ccccataagt atcgggaagg cgccgggcaa gtcggcctgg cgccttcctg
      781 ggcctcagcg ccggacctgt atccctcccg gaccggggta atcggagaca ccttcgaaac
      841 gttacggatc tcttcatcgc gcagaaaccg ttgcgatccc ttatctttgg cagcataatg
      901 tcgccgtcgc agagtcagca ggagatgagg ccgtggttcg aacgaggata ttgttgctcg
      961 gcgcgccgca cgtcgagcac gacggcgtac ctgtcgaggt cgacacgcga aaggcgatcg
     1021 cgctcgtcgc ctacctggcc gtaacgcaac gaggccacac gcgcgacgcg ctcgccggcc
     1081 tgctctggcc cgaatacaac cagagccgtg cccgcgccgc cctgcgtcga actctctcgt
     1141 cgcttggaaa ggcgcgtgcg gagggttggt tgcgcgtcga cagggagggc gttgatctcg
     1201 ccgacgacgc cggtctctgg gtggacgttg acaggtttcg cgggctcctg gccgaatgca
     1261 ggacccacgg acacgctgat cccgaagtgt gcccggaatg cttacccccg cttaccgagg
     1321 cggcgacgtt gtaccgcgac gatttcatgg ccggtttcgg cctgcgcgac agcgtcgcct
     1381 tcgacgactg gcagtttttc cagtcagaga gcctgcgccg agagctggca ggcgccctgg
     1441 agaagctcgc gcgtggccgc ggggcacttg gcgagtggga gtcggccgtc gcccacgccc
     1501 ggcgctggct ggccctcgac accctgcacg aaccggccca ccgtctgctc atggcgctct
     1561 acgcatggtc cgaccagcgc gccgccgccc tccgtcagta cagggagtgc gtccgcatcc
     1621 tcggccagga gcttggcgtg gccccgctag aagagacgac cctgctatac cacgccatac
     1681 aggagaacga tctgccgcca caacctgtcc tctccgaaca tcatcgtccc gccccgcgcg
     1741 aggcgcagtc agtgtccctc gtcgaaacat caaccgtcgc aacatattcc cccgatcacc
     1801 cgctcgtcgg aagggccacg gagtgggcgg ccctgctcgg atcttacggg gcggtcggga
     1861 acaagggccg cgtcgtaatc ctcgaaggag aggccggtat cggcaagacc aggctcgcag
     1921 aggagttcgt ggcccatctg cgcacagaag gcgcgaccgc cgtcgccgcc cgctgttacg
     1981 cgggcgagaa gaacctggcc tacgggcctt tcgtcgaagg tctctcctcc accctcggcg
     2041 gggagaatgc cggccgcctg aagggtttac ccgccggctc gctgcaagag gctgcccgcc
     2101 tcttgcctga cctcgcgtcc ctctccccgg actcgccgcc ggctccgccg ctcgacacgc
     2161 cgggtgccag gagccgcttc ttcggggagc tggtcagggt tttgctcgct gtcctggacg
     2221 gccctccccc cggcatcctc tttctcgacg acctgcactg ggccgacgac gcctctctga
     2281 gtttgctgac ctacctggtg cgtcggttgg aggacaagcc gttactcgtt ctcctcacct
     2341 ggcgggccga ggaggtccgg gaagggcaca ggctgcgagg actgctgacc gaggcaagga
     2401 gatcgggagt agcgacggcc ctgaccctgg agaggttgag cccggcctcg gtcgaggagc
     2461 tggtcgggag cgccgtgccc ggcgcgtgga cacttggctc gcggctcagc gatgagaccg
     2521 agggcctgcc gctcttcttg acggagtaca tggctgccgt cgagaaaggc gagttgagcg
     2581 caggtgacga cgcctgggcg cttcccggtg gggtgcagga tctcctgcgt acccgtctcc
     2641 tgaccgtcgg tgagaccgcg gggcaggtgc tcgccgcggc cgcggtcgtc ggccgatcct
     2701 tcgacttcga cacggtcagg gcggccagcg gcaggggcga ggaagagacc ctgacggccc
     2761 tcgaagagct gagctcgcgc ggtctgatcc gggaggtagg tagcgctgca ggaggggcac
     2821 cggcctacga cttcgaccac gataagctgc gaaccctggt ctacgaggag acgagcctgg
     2881 cccgcaggcg tctcctgcac cgtcgcgccg cggccgcgct cgcgggacag gcccgcggac
     2941 gcgaagcgga ctccctaacc ggccagatag cccgccacca caggctggca ggtcaggacg
     3001 aggaggccgc gcaatattac agactggcag gagattacgc gcggagcctg cacgccaata
     3061 gtgacgccct cgcccactac gaggaggcgc tggcgctggg ataccccgac accaccacgc
     3121 ttcacgaagc catcggggac ctgcgtactc gcgccggcga gtacggcgcg gcgctcgcca
     3181 gctacgaagc ggcggcctcc tcgtccgacg cgggcgacct gtccatactc gaacacaaga
     3241 tcggcaacgt ccacgcccgc aggggcgagt gggacctcgc caggagccat taccagttgg
     3301 ccctcgaagc gttcggggag caagagggag gggcagagct cgcccggctc tacgccgact
     3361 ggagcctgct ctcccacggc caggacgagc gagacgaagc cacagagttc gcaagcaggg
     3421 ccctggaact ggcagaaagc gcgggcgata cgcgggctct cgcccaggcc cacaacatgc
     3481 tcggcatcct ggccggcaga tccggcgacc aggacgccgc gctgcgccac ctccaggtca
     3541 gccttgacct cgcggagacg ctaggcgact cggacgcgaa ggtggccgcc ctcaacaacc
     3601 tggcactcgc ccgcgaacac ggcggggagc cgggggaggc catgaagctc gccgagaccg
     3661 cccttgcgtt gtgcgtggcg tcaggcgacc gtcacaggga ggccgcgcta cacaacaacc
     3721 tggcagacct gcttcacgcc gaaggccgcg agcaagaatc gatggcgcac ctcaagcggg
     3781 ccgttgagct cttcgccgag atcggcgagg aagacgaact gcagccggag atctggaaac
     3841 tcgtggaatg gtagacgaac cggagaccga acctatcccc gccgacctgc ttcgcctccg
     3901 ttatctgaag gtgcgaatgc ctgtgctttg acggcgttga tctcatccca ggggatgcgg
     3961 gggcgcgcga tcccgcgctt gtccagatag cgcgcggcgt tcctacgtga gctggtaaag
     4021 aagcgcacca tcgcgggaag tcccccgata ccggctccag ggatgaaccg aaggggctcc
     4081 agtttccatc cccgctcgtc tctgccaagg acgccgcgta cgaggaagct cttgaggtgc
     4141 cacaagtttt cgtcgtgttt gtggcagagc aggcccgcgg agccgggctg gagaggggtg
     4201 tcggcgggta gctggacccg cagcacctcc gctcccgcag gatcagggta cggccagcaa
     4261 cgcacgctga aagggtagcc ttcgatatcc acgcccgtca ggacggcgct ccggaagtca
     4321 gggaggtact cattgatttc atcccacatg gtccacctcg aacggcgact gcgaaaaatt
     4381 gcacttcggc caccacagga tccggcgcgg cgtcacgtgg atcacgagcc gcatgtagta
     4441 ccagtccatg aagtagcgca tgagcgggtt gctgctgtac acgcgggaag acggctgtcg
     4501 ctggtagacc atgcgcaact cgtcctcgaa tccctcgacc gaggtcatta tctcatcagc
     4561 ggcttcggcg tcggcctgca cgagtacggc gggcgggtcc ttcaggccgc tggccgtcgg
     4621 gttggagaag aggagagaga cgcgcgggtt gcggcggacg ttggacacct tctgagagag
     4681 ggaaatcgag gtcgttatca tgaaacgtcc ctcatcgggc ttccagaagg gcagcgtggg
     4741 ccaggtgatc ggggtgccgt ccctggc
//
LOCUS       DATAIR010000180         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_328167, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000180 DATAIR010000000
VERSION     DATAIR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328167"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..447)
                     /locus_tag="VHM69_04280"
     CDS             complement(67..447)
                     /locus_tag="VHM69_04280"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX2739640.1"
                     /translation="MYEIFVAARFEAAHRLVGDFGPASRMHGHTYRMEVIVRGQHLGD
                     DVTLYDIGELGQAVEGLAASLDYRDLNEVPGLAGVNTTAEAVADYCWESLASPLRNDG
                     LDSLVVRIWESPDAYAAREDSLAR"
     gene            complement(449..1492)
                     /locus_tag="VHM69_04285"
     CDS             complement(449..1492)
                     /locus_tag="VHM69_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX2739641.1"
                     /translation="MASPIEDNLKTRALWFTAPRSAAIREETVPPPGPGEVRVEAIAS
                     AVSAGTEMLVYRGEVPEDLVLDLPTLEGSYAFPIKYGYAAVGRVLDTGSHVEHLSPGD
                     AVFVHHPHQEIFVVPARMPVRIPDHLDPSLGVFVANLETALNIVHDTPVRLGETALVF
                     GQGVVGLLVARLLKLAGAEQVLVVDPIEKRRNLALTAGADGAFEPEVLNERGMEITGG
                     RGADVAVETSGSGAALQSAIDTVATEGTVVVASWYGTKPVTLALGGHFHRGRVRLRSS
                     QVGRLNPGLAPRWDRARRMETVIGLLDRLELGDLISHRIPFDGAPEAYRLIDERPGEA
                     VQVVFTYESSRGG"
     gene            complement(1470..2195)
                     /locus_tag="VHM69_04290"
     CDS             complement(1470..2195)
                     /locus_tag="VHM69_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX2739642.1"
                     /translation="MIGKEQVGEVVTENSVRPAVTVSYAQTLDGRVATVTGESKWISC
                     PDSLRFAHEMRAGHDAVLVGAGTVCKDDPRLTVRHVPGKSPLRVVVDSTMRTPPGAAV
                     LADGAAAGTLLAVTERAPTDRREHALALGATVLILPSDLDGRVDLEALLDRLAALGVG
                     TVMVEGGATMITAFLRGRLADRLAVCVAPKILGAGIDAVGDLGIRDLSRSVILTDTAV
                     EPYGVDLILSGRVEYPDGVSDRG"
     gene            complement(2192..>2438)
                     /locus_tag="VHM69_04295"
     CDS             complement(2192..>2438)
                     /locus_tag="VHM69_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739643.1"
                     /translation="EMLLDLGVRRAAVLTNNPAKIDGLRCHGIEVVERLPVQVEPNPF
                     NLGYLRTKKEKLGHLFPVISGGPPDERSGGDSSKGVS"
BASE COUNT          431 a          792 c          777 g          438 t
ORIGIN      
        1 ggatgcggaa gcggataatt gtgtttgtcg aatgttccta tatagtgacg aaggcgacga
       61 gcacctctat cgggccagcg agtcttcgcg ggcggcgtag gcgtcaggag actcccagat
      121 gcgtaccacg agcgagtcga gcccgtcgtt gcgcagaggc gaggccaaac tctcccagca
      181 gtaatctgcc acggcctcgg ccgtcgtgtt gacgccagcc aggccgggga cctcgttcag
      241 atccctgtag tcgagggagg cggcgagccc ttccacggcc tgaccaagct cgccgatatc
      301 gtagagcgtg acatcgtctc cgagatgctg gccgcgtacg atcacttcca tgcggtaggt
      361 gtgtccgtgc atcctgctcg caggtccgaa gtccccgacg agcctgtgtg cagcctcgaa
      421 ccgcgcggcg acgaagatct catacatcct accctcctcg ggaactctcg taggtaaaga
      481 caacctggac agcctctcct ggtctctcgt cgattagcct gtacgcttcc ggggccccgt
      541 cgaacgggat acggtgcgag atgagatccc ccagttcgag cctgtctagc agaccgatca
      601 ccgtctccat acgcctggcc cggtcccacc tcggcgcaag cccggggttc agccgcccga
      661 cctgcgagga gcggagccta acccttcccc tgtgaaaatg tcccccgagc gcgagcgtga
      721 ctggcttcgt gccgtaccac gaagccacga cgacagtacc ttcggtcgcc acggtgtcaa
      781 tggcactctg caatgcagcc ccggaaccac tggtctcgac agccacgtca gcccctcgtc
      841 cgccggtgat ctccattcca cgctcgttca gcacttctgg ctcgaacgcg ccgtcggcgc
      901 cagcggtgag cgccaggttg cggcgtttct cgattggatc tacgacgagc acctgttcgg
      961 cgcctgccag cttcaggagc cgggccacga gcaacccgac gacgccctgg ccgaagacca
     1021 gcgccgtctc tcccaggcga acgggcgtgt cgtggacgat gttcagagcc gtctccaggt
     1081 tggcgacgaa gaccccgagg gatgggtcca gatgatccgg aattcgaacg ggcatccgag
     1141 cggggacgac gaatatctcc tggtgcggat ggtggacgaa gacggcatcc cctggagaaa
     1201 ggtgctcgac atgggagcct gtgtcgagga cgcgtcccac agcggcgtag ccgtatttga
     1261 tcgggaaggc gtagctacct tcgagcgtcg gaagatccag caccaggtcc tccggtacct
     1321 cgccgcggta gacgagcatc tccgtgcccg cgctcactgc ggaggcgatg gcctcgacgc
     1381 gcacctcgcc aggtccggga ggtggaactg tctcctcacg gatcgcggcg ctacgcggtg
     1441 ctgtgaacca gagcgccctg gtcttcaggt tatcctcgat cggagacgcc atcagggtat
     1501 tcgacgcgtc cgctgaggat cagatctacc ccgtaaggct ccacggcggt gtcggtgagg
     1561 atgacggacc tggacaggtc gcggatacca aggtcgccga cggcgtctat gcctgctccc
     1621 agaatcttgg gcgctacgca aacggccagc cggtcggcca gccttccgcg gaggaaggcg
     1681 gtgatcatgg ttgcccctcc ctcgaccatc accgtcccga caccaagcgc cgcaagcctg
     1741 tcgagcaggg cttcgaggtc cacgcggcca tcgaggtcgg agggcaggat caggacggtc
     1801 gcaccaagtg cgagagcatg ttcgcgtcga tctgtgggtg cgcgttcggt aaccgcgagc
     1861 agcgttcctg cggcggcgcc gtcagcgagc acggccgcgc cgggtggcgt gcgcatggtg
     1921 ctatccacga ccacgcgcag ggggcttttg ccaggcacgt gacggaccgt cagacgggga
     1981 tcgtccttgc agaccgtacc tgctccgacg agcacggcat cgtggccggc gcgcatctcg
     2041 tgggcgaaac gcagggagtc ggggcagctg atccacttgg attcccccgt gacggtagcc
     2101 acgcgcccgt ctagcgtctg ggcgtagctc acggtgacgg caggacgtac ggaattctcc
     2161 gtcaccactt cgccgacctg ctcttttcct atcacgacac ccctttcgaa gagtcgccgc
     2221 cggaccgttc atccggcggt cctccagaaa tgacggggaa gagatgtccg agcttctcct
     2281 tcttcgtccg tagatatccg aggttgaagg ggttcggttc gacctgtacg ggcaggcgct
     2341 ccaccacctc gatgccgtgg caccttaggc cgtcgatctt ggccgggttg ttagtgagca
     2401 cggcggctct tcgcacgccg aggtcaagga gcatctcg
//
LOCUS       DATAIR010000181         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_380453, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000181 DATAIR010000000
VERSION     DATAIR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380453"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..556)
                     /locus_tag="VHM69_04300"
     CDS             complement(236..556)
                     /locus_tag="VHM69_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739644.1"
                     /translation="MQAHGAAQGVGTIPRRRRLEVLLAIAVIVAIVALTALLVRGVPP
                     ATSGVGAGDANLKAGAGTAVVHDDAGNMPPGAGAAVVHDDAGNMAPGADAVVVHDDAG
                     NMKP"
     gene            complement(791..1195)
                     /locus_tag="VHM69_04305"
     CDS             complement(791..1195)
                     /locus_tag="VHM69_04305"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2739645.1"
                     /translation="MGRRVAACLLDSCLVLLVSCPAFAPLLFVRPSISAILSVLLLFL
                     LVNLVVYLGYLVVLEGLSGRTLGKMLWDIEVVRAEGGGPPGTGRSALRALMFLAVDGV
                     AGLFVILASSRHQRLGDMAANTLVVRRHSRIT"
     gene            complement(1286..2242)
                     /locus_tag="VHM69_04310"
     CDS             complement(1286..2242)
                     /locus_tag="VHM69_04310"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX2739646.1"
                     /translation="MSRVAVLRGGHSMERDVSFLTGRRVQHALERLGHEVHPLDIEDS
                     TTGTLMEISPDAAFICLHGPGGEDGTVQALLETLGIPYTGSGPLSSIRCMDKDYAKRA
                     IRASGIQTPPFRTFLRRAMNEMGASGALDVAADDLGYPLVVKPAREGSSFGLSRVEDP
                     EGLLDAVYEALGYDAKILLERWVDGTEISIPIIEPAGEEPKALGLVEIRPREGAYNFE
                     AKYTPGATDFAIPAEIPPEAAARVEETALTVYRTLGCSGFARVDTIYAGGTPWVLDIK
                     TIPGFTETSTFPLAAEAAGISFEKLVETILDAALQTEATAKL"
     gene            complement(2245..3549)
                     /locus_tag="VHM69_04315"
     CDS             complement(2245..3549)
                     /locus_tag="VHM69_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2739647.1"
                     /translation="MPLVDLDRFDFLYANRVKGMKSAATRDLMATLSRPGIISLAGGF
                     PDTRAFGEEAFREISRNIASDAAQALQYGPTAGLEAVKDVIVEVMGAEGTPARQEDVF
                     VTTGAQQGLDLIAKVFLDEGDAVLCEGPTYAGALNAFAAYRPRIAHVPMDRAGIIPVA
                     ARETLKRARKGGIHVKFMYTVPNFQNPAGVTMVAERRRELLDMAREFDLIIVEDNPYG
                     MLRFEGEPLPTLAALEQEEGDLDRVVYLGTFSKIFAPGVRLGWVHAQPGILHKINVGK
                     QGADLCSSNLSQMMISSYFQNADWRAYVRRLTSMYKERRDAMLDALVEFMPKEVHWTH
                     PEGGLFVWATLPSYLDATAMLPRAIAKNVAYVPGEGFYGGNPGTGKNNMRLNFSFVEP
                     ERIRRGIELLSEVIRERMELRSDLERGSHRGSAHRSTVGTDG"
     gene            complement(3553..4137)
                     /locus_tag="VHM69_04320"
     CDS             complement(3553..4137)
                     /locus_tag="VHM69_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739648.1"
                     /translation="MHEVRVESLDKLETQGGQMRLSANAGSLRITFSQEVWEDLISNA
                     PAFEEPTPEELEELDLMKQQLERYARQRSFSTVHRGVRCGSSGLDYDLVLRPAGLFAM
                     TTGYHFTPLVGPELVAKIGAGLEDDKSVEQAFLVGAVADDAALAAGDESLRVLSVRGR
                     SCSTSRFEMPPELWQVLSGRLGWRNVETMTRGER"
     gene            4186..>4405
                     /locus_tag="VHM69_04325"
     CDS             4186..>4405
                     /locus_tag="VHM69_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX2739649.1"
                     /translation="MEAIFVTTNEHKRREVQEILGVELERGDLDLPEIQAIDPAGVAA
                     EKARAAREALGEPESPIIVEDSGLMVDAW"
BASE COUNT          777 a         1407 c         1397 g          824 t
ORIGIN      
        1 ctgaggaacg acctgatagg cgtagtgagg ggcaccatgc agcccgagca cgtctctttg
       61 tggctgcgta ccgatacgtc ctccagagga gagcagccag actagacacc catccgtgtg
      121 aaggggcttg ctcagatgac agagggccgg aacaacccgg ccctctgtct ctcccaaagg
      181 ttcgcctctg accctaaccc taccgtcggt agtgctcttg gcccggtgca ctttatcagg
      241 gcttcatgtt acccgcgtca tcgtggacaa cgacggcgtc cgcgccgggg gccatattgc
      301 ccgcatcgtc gtggacgacc gcggcgcccg cgccaggagg catattgcca gcgtcatcgt
      361 ggaccacggc ggtgcccgcg cccgccttga ggttggcgtc ccccgccccg accccgctag
      421 tcgcaggggg gactccccgc accagcagcg ccgtcaacgc cacaatcgcc acgatcaccg
      481 ctattgcgag cagcacctcc agccgcctgc gtcgaggtat cgtgcccacc ccttgtgccg
      541 cgccatgtgc ctgcatcgca gttcctcctt tctttggcgg gatactctgc ggctagctta
      601 gcggacgctc gatacaagaa cgttacgaga aacgttacga agagttactt tagaatgttt
      661 ttgagatttt tctggctatt tcggcctatt cagcctttct cgacttgatc gatcccgccg
      721 ttgctcgaga aaggctgaag tgctgagaat ctgccgaagg cggcgtgctg acgggcgcga
      781 atcagaggat ctacgtgata cggctgtgtc tgcggacgac cagggtgttt gccgccatgt
      841 ccccgaggcg ttggtggcgg gacgaagcca ggatcacgaa tagtcccgcc actccgtcga
      901 cggccaggaa catgagcgcc cggagcgccg atctgccagt gcctggcggt ccgccgccct
      961 cggccctgac cacctctatg tcccagagca tcttacccaa cgttctccct gacaggcctt
     1021 cgagcacgac caaatagccg aggtagacga ctaggttaac gagaaggaag agaagaagga
     1081 cggagaggat cgcggagatg ctcggccgca cgaacaggag cggcgcgaac gccggacaag
     1141 atacgagtag caccaggcaa ctgtcgagca gacaagccgc taccctgcgg cccactacat
     1201 tcgtatcacg cgccgaaata tcaggctcct gcgttttgta tgctcgcttt cagttctcgc
     1261 ggtcttcggg agatacgcgc cgtctctaca gcttggccgt tgcttccgtc tgcaacgccg
     1321 cgtccaggat cgtctctacc agcttctcga aggatatgcc cgcggcctcc gccgcgaggg
     1381 ggaaggtgga ggtctcggtg aagccgggga tggtcttaat gtcgaggacc caaggggtgc
     1441 ctccggcgta gatcgtatcc accctggcga aacccgagca gccaagcgtt cgatagacgg
     1501 tgagcgccgt ctcttcgacg cgggctgcgg cctcgggagg tatctcggcg gggatggcga
     1561 agtccgtcgc gccgggggtg tatttggcct cgaagttgta ggcgccttcg cgaggtcgga
     1621 tctcgaccag tccgagcgct ttcggctcct cgcccgccgg ctcgattatg gggatggaga
     1681 tctccgtccc atcgacccag cgttcgagca gtatcttggc gtcgtagccc agcgcctcgt
     1741 agacggcgtc gagaagtcct tcggggtcct cgacccgcga gagcccgaag ctggaaccct
     1801 cgcgcgccgg tttcacgacg agcgggtaac cgaggtcatc ggctgccacg tcgagcgccc
     1861 cggaggcgcc catctcgttc atcgcccttc tgaggaacgt gcggaagggc ggggtctgta
     1921 tgccggaggc acggatcgcg cgcttggcgt agtccttgtc catgcagcgg atggacgata
     1981 ggggcccgct gcctgtgtac gggatgccga gggtctcgag taacgcttgc acagttccgt
     2041 cctcgccgcc gggaccgtgc aggcagatga aagccgcatc cggcgagatc tccatcagcg
     2101 ttcccgtggt cgagtcctcg atatcgaggg gatggacctc gtggccgaga cgttcgaggg
     2161 cgtgctggac gcgacgaccc gtcaggaagg agacgtcgcg ctccatcgag tgcccgccgc
     2221 gcagtaccgc aacgcgtgac acctctagcc gtccgtgccg actgtcgagc gatgcgcgga
     2281 gcccctgtgc gagccgcgtt ccaggtcgct gcgcagttcc atacgctccc tgatcacctc
     2341 tgagagaagc tctatgccgc gccggatcct ctccggctcc acgaaggaga agttgagcct
     2401 catgttgttc ttgcccgtcc ccggattgcc cccgtagaac ccttcgccgg gcacgtaggc
     2461 gacgttcttc gctatggcgc gcgggagcat cgcggtcgcg tcgaggtacg aagggagggt
     2521 ggcccagacg aagagcccgc cctcggggtg ggtccagtgg acctccttcg gcatgaactc
     2581 cacgagggcg tcgagcatcg cgtcgcggcg ctccttgtac atcgaggtca ggcgcctgac
     2641 gtacgctctc cagtccgcgt tctggaagta agaggagatc atcatctgcg agaggttcga
     2701 cgagcacagg tcggcgccct gcttgccgac gttgatcttg tgcagtatcc caggctgcgc
     2761 gtgcacccac ccgagcctga cgccaggggc gaagatcttg gagaacgtcc cgagatatac
     2821 gactcggtcc agatcgccct cttcctgctc cagcgccgcc agagtgggga gcggctctcc
     2881 ctcgaagcgg agcatcccgt atgggttgtc ctcgacgatg atcaggtcga actctcgcgc
     2941 catgtcgagc agctctctgc gcctctctgc gaccatcgtc acgcccgccg ggttctggaa
     3001 gttcgggacg gtgtacatga acttgacgtg aatccctcct ttgcgggctc tcttgagggt
     3061 ctcgcgggcc gcgacgggga tgatgcccgc cctgtccatc gggacgtggg ctatgcgcgg
     3121 cctgtacgcg gcgaaggcgt tcagagcccc ggcatatgtc gggccctcgc agagcacggc
     3181 gtcgccctcg tcgaggaaga ccttggcgat gaggtcgagg ccctgctggg cacccgtggt
     3241 gacgaagacg tcctcctgcc tcgcgggcgt gccctccgcg cccatgacct ccacgatgac
     3301 gtcctttacg gcttcgaggc ctgcggtcgg cccgtactgc agggcctggg cggcgtctga
     3361 ggcgatgttg cgtgagatct ctcggaacgc ctcctcgccg aaggccctcg tgtccgggaa
     3421 gccgccggcg agcgagatga tgcctgggcg cgacagcgtg gccatgaggt cgcgggtcgc
     3481 agcggacttc atgcccttga ccctgttggc gtagaggaaa tcgaagcggt cgaggtctac
     3541 gagcggcaag ggttatctct cccccctggt catggtctca acatttctcc agccgagcct
     3601 ccctgagagg acctgccaga gctcgggcgg catctcgaag cggctcgtgc tacacgagcg
     3661 accacggacg gagaggaccc tcaggctctc gtcacccgcc gcaagggccg cgtcatcggc
     3721 cacagcccct acgaggaaag cctgctcgac agacttgtcg tcttcgagac cggccccgat
     3781 cttcgccacc agttcgggtc cgacgagggg cgtgaagtgg tagcctgtcg tcatggcgaa
     3841 gaggcccgcc ggacgcagca cgaggtcgta atccagaccc gaactcccgc acctcacacc
     3901 tctgtgcacg gtgctgaagg accgctgtct cgcgtaccgt tcgagctgtt gcttcatgag
     3961 gtccagctct tccagctcct caggagtcgg ttcttcgaag gccggggcgt tggagatgag
     4021 gtcctcccag acctcctggg aaaaggtaat gcgcagggaa ccggcgttgg ccgagagtcg
     4081 catctggcct ccctgcgtct ccagtttgtc gaggctctcc accctcacct catgcatgga
     4141 cgcgactgta tcataaagaa tcgctcttat gtagactctt acgcagtgga agccatcttc
     4201 gtgaccacaa acgagcacaa gcgacgcgag gtgcaggaga tcctcggcgt cgagctggag
     4261 aggggggatc tcgacctgcc cgagatacag gcgatcgacc ccgccggtgt agcagctgag
     4321 aaagctcgcg cagcccgcga agccctaggt gaaccagaat ctcccatcat cgttgaagac
     4381 tccggactca tggtagatgc gtggg
//
LOCUS       DATAIR010000182         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_11433, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000182 DATAIR010000000
VERSION     DATAIR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11433"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VHM69_04330"
     CDS             complement(<1..556)
                     /locus_tag="VHM69_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739650.1"
                     /translation="MPYASNVLDGNLVYFEDDGGDGEAVVLHGGLVDSVELVRASNIA
                     QALQELPEEFRLIYVDHRGVGRSDKPHEVEAYAMPLRVADAVAVLDELNIERAHFIGN
                     SWGGRLGFGIGEHAPERVLSLVIGGQQPYAIRLDGPLARAVTETLAESRRKGSLEPFV
                     DMLESFAGGRLPDALRERWLDNDPV"
     gene            complement(703..1494)
                     /locus_tag="VHM69_04335"
     CDS             complement(703..1494)
                     /locus_tag="VHM69_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739651.1"
                     /translation="MASPRGHERAAGTSERAYRSLLRAYPRELRDEYGGEMARCFQDL
                     CREELEDRGGLGLAALWANTLPDLIYTALKERSTMLARNTYRSVVGVALATAFILLIP
                     LLAAPAWSLADFVTAGAVIFGTGLTYVLVARKAGNIAYRAAVGVALAAAFLLVWINLA
                     VGIIGHSGDLANSMYVGVLAVGIIGAIIARFQPRGMARTLFATALAQALVAVIVLIFG
                     LGLPWSPPVEILGLTGFFVALFVGSALLFRYAAREQPPAGVGQEG"
     gene            complement(1494..1886)
                     /locus_tag="VHM69_04340"
     CDS             complement(1494..1886)
                     /locus_tag="VHM69_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2739652.1"
                     /translation="MGGKDVSGERSRDPGDLLPLTPVALNVLLALADGERHGYGIMLE
                     VRERTGGQVRLGPGTLYGAIKRLKEGGVIEESGERTEADDERRRYYRLTGFGGEVLAA
                     EVERLDGLVRAARRKGAFPAPKPSPEGV"
     gene            2351..>3198
                     /gene="glgP"
                     /locus_tag="VHM69_04345"
     CDS             2351..>3198
                     /gene="glgP"
                     /locus_tag="VHM69_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2739653.1"
                     /translation="MMQDVPRRLERLPELAHNLFWTWQPETQDLFKRLDPELWEVTEH
                     NPVRLLKETTNLEAAAGDADFVEAYNRTLRDLDGYLGRRDTWMAHTYPDFPGPVAYLS
                     AEFGIHESLPIYSGGLGILAGDHLKSASDLGVPLFGVGILYAQGFFRQRLDAEGRQQE
                     VYEPLEPENRPVATALDVDGREVLVSVELPGRELLLKVWKAEVGRVSLFLLDADLPEN
                     APEDRALTARLYGGDLRTRIAQELVLGVGGVRALRSVGIHPAVYHMNEGHAAFSGLER
                     MREFVSA"
BASE COUNT          604 a         1099 c          934 g          561 t
ORIGIN      
        1 ccacagggtc attgtcgagc caccgctccc gcagagcgtc tggcaaccgg ccaccagcaa
       61 acgactccag catatcgacg aaaggctcca aactaccctt ccttcgcgac tcggccagag
      121 tctccgtgac tgcgcgagcg agcggtccat cgaggcggat ggcgtacggc tgctggcccc
      181 cgatcacgag cgaaagcacc cgctcgggag catgttcgcc gatgccgaag ccgagcctcc
      241 ctccccatga gttgccgatg aagtgtgcgc gctcgatgtt gagttcatct aagaccgcca
      301 ccgcatcagc cacacgcaac ggcatcgcat atgcctccac ctcgtggggc ttgtcgctgc
      361 gcccgacacc acgatgatcg acgtagatga ggcggaactc ctcaggcagt tcctgcaacg
      421 cctgggcgat gttcgaggct cgcaccagct caactgaatc aactagccct ccatggagga
      481 cgaccgcctc tccgtcgccg ccgtcatcct caaagtacac caggtttcca tccagcacgt
      541 tgctggcgta aggcatgagt gtccttcccc gctccctacc ggagcgcact gcacctattg
      601 tagcgaagac tgcgaagata atgctactta caagaaatcc atacttctat gcactccggt
      661 gaataggggg ctatgcctga ttctctcgga cgtagctgcg ggtcaaccct cctgccctac
      721 gccagcggga ggttgctccc gcgcagcata ccgaaacaac aacgccgatc cgacaaacag
      781 tgcgacgaaa aacccagtca ggcccaggat ctccaccggc gggctccacg ggagacccaa
      841 cccgaagatc agcacgatca cggcgaccaa cgcctgggcg agcgccgtcg cgaacaacgt
      901 gcgtgccatt ccacgtggct gaaagcgcgc gatgatggca ccgatgattc cgacggcgag
      961 cactccaaca tacatcgagt tggcgaggtc gcccgaatgc ccgatgatgc caacggcaag
     1021 gtttatccag acgaggagga acgctgccgc gagcgcaacg ccgacggcgg ctcggtacgc
     1081 gatattgccc gccttcctcg ctaccagcac atacgtgaga ccagtgccga agatgacggc
     1141 accggcggta acgaaatcgg ccaggctcca ggccggtgcc gctaacaagg gtatcagcaa
     1201 tatgaacgcc gtcgcgagcg caacgccaac gacggatcgg tacgtgttcc tggccaacat
     1261 agtgctcctc tccttgagcg cggtatagat taaatccggc agggtattgg cccacagcgc
     1321 cgccagaccc aggccgccgc ggtcctccaa ctcctcgcgg cacaggtcct gaaaacagcg
     1381 ggccatctcg ccgccgtact cgtctcgaag ctcgcgcggg taggcccgca ggagcgaccg
     1441 gtaggcccgc tccgatgtcc ctgcagcgcg ttcgtgtccc ctcggagagg ccatcacacc
     1501 ccctccgggc tcggctttgg cgcggggaag gcgccctttc ggcgcgcggc gcgcaccagg
     1561 ccgtcaagcc tttcgacctc cgccgccagg acctcgccgc cgaaaccggt aaggcggtag
     1621 tagcggcgcc gctcgtcgtc cgcctcggtc ctctctccgg actcctcgat cacgcctccc
     1681 tccttgaggc gtttgatcgc cccgtagagc gtgcccggcc ctagccgcac ctgccctccg
     1741 gtcctctccc tcacctccag catgatccca tagccgtggc gttccccgtc ggccaacgcc
     1801 aaaagcacgt tcagcgcgac gggcgtcagg ggcagaaggt cgcccggatc tctcgatcgt
     1861 tcccccgaca cgtccttacc acccatgaca ccatccctct gcgttgctcc gttcctcgat
     1921 gcatcgtgag acgatgcatt tcctgcggca tagtacgccg catcggaacg cctgtcaagt
     1981 cgagttaagc gccgacgctg gcggcgtgga gcgcgggatg aagaggaacg ttccgggcgg
     2041 cacactaatc cggcgctcgc cgaataggaa gcgaaactct ctgtcgaaga cgtacaagag
     2101 ttcatcgcga ccagggtgaa tatggcggag tgccaaagtc tccgggacgc tcgacgctac
     2161 gaggaaccag atctagcttc ctgtttcctc gccggtcgct ttcagcgtaa cggcgtccct
     2221 ggttgggttc ggcactttcc ccgcaagcgg ccaggctcgc cttcccgggc gtcgccggac
     2281 atcggttttg caccccgcgc gtttccctat agaatgcaca ctcgttcacc ggagaaggga
     2341 gcgttaaaac atgatgcagg acgtaccccg ccggttggag cggcttcccg agctcgcgca
     2401 caatctcttc tggacctggc agcctgagac ccaggacctg ttcaagcgct tggatccgga
     2461 gctgtgggag gtcacggaac acaatcccgt ccggcttctg aaggagacta ccaacctgga
     2521 agccgccgcc ggggatgcgg acttcgtgga ggcctacaac cgtactttgc gagacctgga
     2581 cggctacctc ggccggcggg acacctggat ggcgcacacc tacccggact ttccgggtcc
     2641 ggtagcgtac ctctcggcgg agttcgggat acacgagtcg ctcccgatct actccggtgg
     2701 attgggcatc ctcgccggcg accacctcaa gagtgcttcg gatcttggcg tccccctctt
     2761 cggagtcggc atcctctatg ctcagggatt cttccgccag cgcctcgacg ccgaggggcg
     2821 tcagcaagaa gtttacgagc ccctggagcc ggagaaccgg cctgtcgcga ccgcccttga
     2881 tgtagacggt cgcgaggtgt tggtgagcgt cgagctgccc ggccgcgaac tgctcctgaa
     2941 ggtctggaag gccgaggtcg gacgcgtctc gttgttcttg ctcgacgcag acttacccga
     3001 gaatgccccc gaagaccgcg cgctcaccgc ccgcctctac ggcggcgact tacgcacccg
     3061 tatcgcccag gagcttgtcc tgggagttgg cggcgtccgg gcgctgcgca gcgtcggtat
     3121 ccacccggcc gtttaccaca tgaacgaagg tcacgccgcc ttctccggcc tcgagaggat
     3181 gcgcgagttc gtctctgc
//
LOCUS       DATAIR010000183         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_11597, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000183 DATAIR010000000
VERSION     DATAIR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11597"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1335)
                     /gene="aroA"
                     /locus_tag="VHM69_04350"
     CDS             complement(13..1335)
                     /gene="aroA"
                     /locus_tag="VHM69_04350"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX2739654.1"
                     /translation="MNRVAGVPGVDFSVEDVRGTFPDELEIVPLDRPPDATVLIPGSK
                     SVTNRALLIAALADGRSRVSNPLFSDDSYWLMEALVRLGIDVSAERGEVCVSGQGGEI
                     QASGVDLFVGNAGTVARFLPPALALGKGPYTVDGIPRMRERPVEDLVNAMRQLGAAVE
                     YAREDGRFPLVISGGGMKGGTARVSGSKSSQFVSGLLMAAPYAEVPVTLYAEGRKEWP
                     YVGITTTVMRAFGVDVDEGGGRFEVRPAVYSAREYHVEPDASGASYFMAAAAVTGGRV
                     RIPGLGSGSPQGDLRFTGVLREMGCEVEIAHDFVEVQGPDQLRGVEVDMNAFSDTMIT
                     LSAISPFATTPTTINNVGHTRLQETDRLAAVATELERLGVETQQTPTSIRIIPRQVRP
                     AVIETYGDHRMAMAFAVTGLVGGGIRIRDPGCVTKTFPDYFERLESLR"
     gene            complement(1332..2027)
                     /locus_tag="VHM69_04355"
     CDS             complement(1332..2027)
                     /locus_tag="VHM69_04355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2739655.1"
                     /translation="MRLQAFLARAGAVPSRRKAEAPILEGRVKVNGETATLGTTVTQE
                     DEIELDGRPVHLPTDHVYLALNKPAGYLTTMSDDRGRTTVADLMPRVPGLVPAGRLDA
                     DTTGLLILTNDGPLAHRITHPSSEIEKHYRLTLENPVPDDALASLAAGPALEDGKMYP
                     PKLTNLDRTPDTTTLDLTIHEGRNRIIRRACLAVGLRLISLQRTRVGPVALGDLREGK
                     YRALTPDELGALR"
     gene            complement(2027..2623)
                     /locus_tag="VHM69_04360"
     CDS             complement(2027..2623)
                     /locus_tag="VHM69_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX2739656.1"
                     /translation="MSESEHIPPSPRPEHLIEAVLLVSARPVSPSDLRSATGLSEEDT
                     ESALASLTGRYAPESSGIVLRNVAGGYLLSTNPACSGAVERFREEARPTPLSGAAHEV
                     LACALYLGPLTRGAVSRVRGVNSDAVIRNLIERGLLIEVGADYDAPGSPALLGVTDEF
                     LAATGASSPEDFPPVDSLVTQEELTRVRERLVTPPEAG"
     gene            complement(2620..>2837)
                     /locus_tag="VHM69_04365"
     CDS             complement(2620..>2837)
                     /locus_tag="VHM69_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739657.1"
                     /translation="LGPITVTLQELARIIRTALTGGPVSYESLTSDMDRLNKAVAFAA
                     ALSLAQEGSLKLSQTEPLGPLTLEPAG"
BASE COUNT          466 a          862 c          987 g          522 t
ORIGIN      
        1 agcccgcgct cttcaccgta aggattccag ccgctcgaag taatcgggaa aggtcttggt
       61 cacacacccg gggtccctga tccttattcc tccccccacc agccccgtta ccgcgaacgc
      121 catcgccatc ctgtggtcgc cgtaggtctc aattacagca ggccgtacct gtcgaggtat
      181 tatgcggata gaagtagggg tttgttgggt ctcgacaccg aggcgctcga gttctgtggc
      241 gacggcggcg aggcggtcgg tctcctggag gcgggtatgg ccgacgttgt tgatcgttgt
      301 tggagttgtg gcgaaggggg atattgcgga gagagtgatc atggtgtctg agaaggcgtt
      361 catgtcgacc tcgacgccgc gcagctggtc aggtccctgt acttcgacga agtcatgggc
      421 tatctcgact tcacagccca tctcgcgcag cactccggtg aagcgcaggt cgccctgcgg
      481 cgagccggag ccgaggccgg gtatcctcac gcgcccgccg gtaacagccg ctgctgccat
      541 gaagtaggaa gcccctgagg cgtcgggctc gacgtggtat tcgcgcgcag agtagacggc
      601 gggtcggact tcgaagcgtc caccaccctc gtctacgtcc accccgaaag cccgcatcac
      661 cgtggtggtg atgccgacgt agggccactc ctttctgccc tcggcgtaca acgtgaccgg
      721 gacttccgcg tagggggctg ccatgagcag gccgctcacg aactggctgc tcttgctccc
      781 agagacgcgc gccgtcccgc ctttcatacc gccgccactt atgacgaggg ggaaccttcc
      841 gtcctccctg gcgtattcca cggccgcgcc gagctgcctc atcgcattca cgagatcttc
      901 gacgggacgc tcgcgcatcc ttggtattcc gtcgaccgtg tacggaccct tgccaagcgc
      961 gagcgcgggc ggcaagaagc gggcgacggt gccagcgttt cccacgaaga ggtccacgcc
     1021 ggaagcctgg atctcacctc cctgcccgct tacgcagacc tctccccttt cggcgctcac
     1081 gtcgatgccg agcctgacga gggcctccat gagccagtac gagtcgtccg agaagagcgg
     1141 gttgctgact cttgaacgcc cgtcggcgag ggcggcgatg agtagcgcgc gattggtaac
     1201 gcttttggaa ccggggataa gtaccgtcgc gtccggcggc ctgtccaatg ggactatttc
     1261 gagctcgtca gggaaagtgc cgcgtacgtc ttcgacagaa aaatccacgc ccggcacgcc
     1321 ggcgacgcgg ttcaccgcaa agcccccagc tcgtccggcg tcagggcgcg atacttgccc
     1381 tcacggaggt cgccgagggc taccgggcca acgcgcgtcc tctgcagtga gatcaatcgc
     1441 agccccaccg ccaaacatgc tctacgtatt atgcggttac ggccctcgtg gatggtcagg
     1501 tcgagtgtcg tggtgtcggg tgtgcggtcc aggttggtca gtttcggagg gtacatctta
     1561 ccgtcttcga gggccgggcc tgcggcgagg gaggccaggg cgtcgtcggg gacggggttt
     1621 tcgagggtga ggcggtagtg tttctcgatc tccgatgagg ggtgggtgat gcggtgggcg
     1681 agcgggccgt cgttggtgag gatcagaagt cccgtggtat ccgcgtcgag gcggcccgcg
     1741 ggaacgagtc ccggcacgcg aggcatgagg tcggcgacgg tagtgcggcc acgatcgtcg
     1801 ctcatggtcg tcaagtagcc ggctggcttg ttaagggcaa ggtagacgtg gtcggttgga
     1861 agatggacgg gtctgccgtc gagttcgatc tcatcttcct gcgtcacggt cgtgccgagg
     1921 gtagcggtct caccgtttac cttcacgcgg ccctccagga tcggggcctc agccttgcgg
     1981 cgagacggga ccgcgccggc gcgggcgagg aacgcctgca ggcgcattac ccagcctcgg
     2041 gcggggtcac cagacgttcg cgtacgcggg tcagctcttc ctgtgtcacg agcgaatcga
     2101 ccggtgggaa atcctcgggg gaagaagcgc ccgtggcggc caggaactcg tcggtgacgc
     2161 cgaggagagc gggtgatccg ggggcatcgt agtcagcgcc cacttcgatc aggagtccgc
     2221 gttctatgag gttccgaatt acggcgtcgg agttgacgcc tcgcacgcgc gagactgcgc
     2281 cgcgtgtcag cggtccgaga tacagggcac aggccaggac ctcgtgggcc gcgccggaga
     2341 gcggtgtcgg gcgggcctct tcgcggaaac gctccacggc gcccgagcat gcagggttcg
     2401 tggagagcag gtagccgccg gctacgttgc gaagcacgat gcccgaagac tccggcgcgt
     2461 agcgcccggt caggctggcg agagcagatt cagtatcctc ctccgagagt cccgtggccg
     2521 agcggaggtc cgaggggctc acagggcggg cgctcacgag caggacggct tcgatcaggt
     2581 gctcgggccg aggcgaaggg ggaatgtgct cactttcgct caccctgccg gctccagggt
     2641 caggggtccc aggggctcgg tctgggagag cttcaggctt ccttcctggg cgagcgagag
     2701 cgcggctgcg aacgcgacgg ctttgttcag acggtccatg tcgctcgtga ggctctcata
     2761 ggagacaggt ccaccagtca gggcggttcg gataatgcgg gccagttcct ggagggtcac
     2821 tgttatcggc ccgaggt
//
LOCUS       DATAIR010000184         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_144128, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000184 DATAIR010000000
VERSION     DATAIR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144128"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VHM69_04370"
     CDS             <1..1035
                     /locus_tag="VHM69_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX2739658.1"
                     /translation="GSDLHLKVGARPAIRVRGLLTPLESHEVLRPEDTRDILGQMIPE
                     NLVGEFEADGEADFSYAVPGRGRFRVNAFRQRGSVSIVMRFIPFGVPRFEDLNLPDAI
                     RRLAIEERGIVLVTGTTGSGKSTTLASMIDLVNRTMRKHIVTIEDPIEFLHSDQSSLI
                     NQREVGTDTVSFARALRRVLRQDPDIILIGEIRDSETAQIALSAAETGHLVLSTLHTV
                     DATETVNRIIDLFPPHERSQVRTMLAGTLKGIIGQRLIRTKDGGRVAACEIMVTTGRI
                     QDFIMDPAQTGQIQQAISEGEYYGMQTFDQALLKLVEEDRVEYEEALTASSRPQDFKL
                     MVQSLGLPSL"
     gene            complement(1070..3697)
                     /locus_tag="VHM69_04375"
     CDS             complement(1070..3697)
                     /locus_tag="VHM69_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2739659.1"
                     /translation="MSGSETRGATNETLRFLSKASTLLSSSLDYRETLASVARLAVPR
                     LADWCVVDVVEEDGSLQRLAAEHEDPEKVALARRLQERYPPDPDAPYGVNHVVSTGRS
                     ELVPEIPESGLDEVARDEEHRDLLRTLGLKSYMVVPLLARGRMLGAISFVAAESGRRY
                     GKEDLELAEDLARRAALAVDNARLYEEAQNEIAERRRTEEALREAEARKTAIMDAALD
                     CIITMDEAGLVTDFNPAAEITFGHRRDEVLGRELAEVIIPPAFRDAHRRGLAHYLATG
                     DGPVLNRRVELRGMRSDGSEFPVELTIVPIQVAGRPAFTGYLRDITERERAQARLQRA
                     ERLYRTLVDQMPAVTYIEALDAGERETNLVYASPQIEDLFGYSAEEWISAPDLFVRLL
                     HPEDRERVLAADDLTERTGEPFREEYRQFTRDGRVLWIRDEAILVRDEEGNPLYWQGI
                     IMDISEQKRVEEALRTQNEYLASLHETTLGLVERLDPTHLLRGILERAAALVGTTHGY
                     IYLEDEGILQMSLGAGLFKDYVGKQIEPGDGLAGKVFVSAEPLSVDDYRTWENRVELF
                     PDTIRALAGVPLWSGSRVTGVLALSYDDPDRRFGEQEMGVLVRFADLASVALDNAWLH
                     DSARREIAARERTERDLAALVDELRRSNAELEQFAYVASHDLQEPLRMVSSYTQLLAR
                     RYQGRLDEDADEFIGFAVDGANRMQTLINDLLQYSRVGTRGRDLAPTETGAILDAACA
                     NLRVLLEESDAKVTSDELPIVMGDPVQLSQLFQNLIGNAVKFSKEGEQPRIHVGAERR
                     DGDWLFSVRDNGIGIEPEYRERIFVIFQRLHARTEYSGTGIGLAVCKKIVERHGGKIW
                     VDSVPGEGSTFYFTLRAGE"
     gene            complement(3694..>5256)
                     /locus_tag="VHM69_04380"
     CDS             complement(3694..>5256)
                     /locus_tag="VHM69_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2739660.1"
                     /translation="LLDLSLPDSAGLQTVERMRRAAPELPLVVLSGRDDEEIALQALQ
                     GGAEDYLVKGQGDGDLISRSIRYSIERKIAEEKLARLAQFDPLTGLANRALFHDRLEH
                     AVARVERHGGMLALLFLDLDRFKAVNDTVGHIGGDALLTQVARRIDARVRESDTVARL
                     GGDEFAIIIEDLVEAREAARVAEDLIDIISEPFVVNGHEIPIGVSIGIAVSPPSMGDR
                     LLKDADAAMYRAKGEGGNDHQFYTEEMNVQASERLLLERDLRHALDREEFLLYYQPQV
                     DLATGDIVGAEALLRWHNHERGLVSPSDFIPVLEESGLIIPVGAWVLEEACRQASSWH
                     DSGLAPVTVAVNLSARQLDRGSLIYAVARAVEESGLDPGYLELEITESLLVRHSEAGL
                     RSVELLKKAVNGLKISIDDFGTGYSSLYRLKTLPIDRLKIDRSFIRGVTTDPEDSAIT
                     ATVISLAHELHLQVTAEGVETAEQLAFLRERGCDEAQGFYFSRPLPAHEFVRLLDGTR
                     NLLGASGEVSGR"
BASE COUNT          889 a         1769 c         1647 g          951 t
ORIGIN      
        1 ggcagcgacc tgcacttgaa ggtgggtgcc agaccggcga tccgggtacg cggtctcctc
       61 acgcccctgg agagccatga ggtgctgcgt cccgaagaca cgcgggacat cctcgggcag
      121 atgatccccg agaacctggt cggcgagttc gaggcggatg gggaggctga cttctcctac
      181 gccgtgcccg gaaggggccg cttcagggtc aacgccttca gacaaagagg ttccgtgtcc
      241 atcgtcatgc ggttcatccc gttcggggtg ccgaggttcg aggacctgaa tctgcccgat
      301 gcgatccgca ggctcgccat cgaggagcgt gggatagtac tcgtcacggg cacgacgggg
      361 agcggcaagt ctacgacgct ggcctcgatg atagacctcg tcaaccgcac gatgcgcaag
      421 cacatcgtca ccatcgagga cccgatagag ttcctgcaca gcgatcagag cagcctcata
      481 aaccagcgcg aggtcggcac ggacaccgtc tccttcgcgc gcgccctcag gcgcgtcctc
      541 aggcaggacc ccgacataat cctcataggc gagatcaggg actccgagac agcccagatc
      601 gccctctcgg cggctgagac ggggcacctc gtcctctcca cgctccacac tgtcgacgcg
      661 acggagacag tcaaccgcat catcgacctc tttcctccgc acgaacggag ccaggtgcgc
      721 accatgctcg caggcaccct caaaggcatc atcgggcagc gattgatccg caccaaggac
      781 ggcggacgcg tcgcggcctg cgagatcatg gtcaccaccg ggcgcatcca ggacttcatc
      841 atggacccgg cgcagaccgg ccagatccag caggcgatct ctgagggcga gtactacggt
      901 atgcagacct tcgaccaggc ccttctgaag cttgtggagg aggacagggt cgagtacgag
      961 gaggcgctca cggcgtcgag ccgtccgcag gacttcaagc tgatggtcca gtccctcggt
     1021 ctccccagct tgtagggaag ggttctcata gagatcgact tttcgcaccc tactcaccgg
     1081 cccgcaacgt gaagtagaag gtgctgccct cgcctggcac cgaatcgacc catatcttcc
     1141 cgccgtggcg ctcgacgatc ttcttgcaga ccgccaagcc gatgcctgtc cccgagtatt
     1201 ccgtcctcgc gtgcagacgc tggaagatca cgaagatcct ctcgcggtac tcgggctcga
     1261 tgcctatgcc gttgtctctc accgagaaga gccagtcacc gtcgcgccgc tctgccccga
     1321 cgtggatcct cggctgctcc ccttccttgc tgaacttgac ggcgttgcct atcaggttct
     1381 ggaacaactg cgagagctgg acggggtcgc ccatcactat cggaagctca tcggatgtga
     1441 ccttcgcgtc gctctcttcc aggagcaccc gcaggttcgc gcacgccgcg tcgaggatcg
     1501 cccccgtttc cgttggtgcg agatccctac cgcgtgtacc gacccgagaa tattgcagga
     1561 ggtcgttgat gagggtctgc atccggttcg cgccgtccac ggcgaagccg atgaactcgt
     1621 cggcgtcttc gtccagccgg ccctggtaac gtcgggcgag gagctgggtg tagctcgaca
     1681 ccatcctcag cggctcctgc aggtcgtgtg acgcgacgta cgcgaactgc tccagctcgg
     1741 cgttcgaccg ccgcaactca tcgacgaggg ctgcgaggtc cctctcggtc cgctcgcgcg
     1801 cggcgatctc ccgcctggcc gagtcgtgca gccaggcgtt gtccagtgcc accgaggcca
     1861 ggtcggcgaa gcgcacgagc acccccatct cctgctcgcc gaacctgcgg tcaggatcgt
     1921 cgtaagagag ggccaacacg cccgtcaccc tgctccctga ccagagaggg acccctgcca
     1981 gcgcgcgtat ggtgtcgggg aagagctcga ccctgttctc ccaggtcctg tagtcgtcca
     2041 cgctcagcgg ctccgcgctc acgaagacct tgccagcgag cccatcgccg ggttcgatct
     2101 gcttccccac gtagtctttg aagagccctg cgccgagact catctgcaaa attccttcgt
     2161 cctccaggta gatgtagccg tgcgtggtgc cgaccagcgc ggctgcccgc tccaggatgc
     2221 cgcgcaggag gtgcgtcgga tcgaggcgtt cgaccaggcc aagggtcgtc tcgtgcaggc
     2281 tcgcgaggta ctcgttctgc gtgcggagcg cctcctcgac ccgcttctgc tcggagatgt
     2341 ccatgattat gccctgccag tacagcgggt tcccttcttc gtcacgtacg agaatggcct
     2401 cgtccctgat ccacaggacc ctgccgtccc tggtgaactg gcggtactcc tcacggaacg
     2461 gctcgcccgt gcgctccgtg agatcgtcgg cagcgagcac ccgctcgcgg tcttcggggt
     2521 gcagcagcct gacgaagaga tcgggggcag agatccactc ctccgccgaa tagccgaaga
     2581 ggtcttcgat ctgggggctc gcgtacacga gattcgtctc gcgttccccc gcgtcgagcg
     2641 cctcgatgta ggtgaccgct ggcatctgat cgacgagcgt ccggtacagt ctctccgccc
     2701 tctgcagccg tgcctgtgcc cgctcgcgct ctgtgatgtc gcgcagataa ccagtgaacg
     2761 cgggccgccc ggctacctgt atggggacta tggtcagctc gacggggaac tcgctcccgt
     2821 ctgaccgcat cccccgcaac tcgacccgac ggttgagtac gggtccatcc cccgtcgcca
     2881 gataatgagc caggccccgg cggtgcgcgt cacggaaagc tggagggatt atcacctcgg
     2941 cgagttccct accaagtacc tcgtcgcgcc tgtggccgaa cgtgatctcc gcggcggggt
     3001 tgaagtccgt gacgagacct gcctcgtcca tggtgatgat gcagtcgagg gcggcgtcca
     3061 tgatcgctgt cttgcgcgcc tcggcctccc tgagtgcctc ctccgtcctc cggcgctcgg
     3121 cgatctcgtt ctgggcctcc tcatagagac gggcgttgtc cacggcgagc gccgcgcggc
     3181 gggcgaggtc ctccgcaagc tccaggtctt ctttcccgta gcgcctcccc gactcagcgg
     3241 cgacgaagga gatcgcgcct agcatccgcc cccgagcgag gagcggcacg accatgtacg
     3301 acttgaggcc gagcgtcctc agcaaatctc gatgctcctc gtcgcgggcc acctcgtcga
     3361 ggcctgactc ggggatctcg ggtacgagtt cagaccggcc cgtgctgacc acgtggttca
     3421 cgccgtacgg cgcgtcagga tcgggcgggt agcgttcttg caggcggcgg gcgagggcga
     3481 ccttctcggg gtcctcgtgc tcggcggcca gccgctgcag tgagccgtcc tcctcgacga
     3541 cgtcgacgac acaccagtcg gcgaggcgcg gcacagcaag gcgcgccacg ctcgcgagtg
     3601 tctccctgta gtcgagggag gacgagagca gcgtgctcgc cttcgacagg aaacgtaacg
     3661 tctcgttggt cgcgccgcgc gtctccgagc cgctcacctc cccgagactt cgccggacgc
     3721 gccgagcagg ttgcgcgtgc cgtcgagcag cctgacgaac tcgtgcgcag ggagcgggcg
     3781 gctgaagtag aagccctggg cctcgtcgca gccccgctcg cgcaagaagg cgagttgctc
     3841 ggcggtctcg acgccctctg ccgtcacctg gaggtgcaac tcgtgggcga gcgagatcac
     3901 cgtcgctgtg atcgccgagt cctcaggatc ggtcgtaaca cccctgatga acgagcggtc
     3961 gatcttcagc ctgtcaatgg ggagagtctt gagccgatag agcgaagaat agccggtccc
     4021 gaagtcgtct atggagatct tcagcccgtt cacggctttc ttgagcagct ccaccgacct
     4081 cagaccagct tcggaatgcc tcaccaggag gctctcggtt atctccagct cgaggtatcc
     4141 agggtcgagg ccgctctctt cgacggctcg cgcgacagcg tatattaggc tcccgcggtc
     4201 gagctggcgg gccgagaggt tgaccgcgac cgtgaccggc gccaggcccg agtcgtgcca
     4261 cgagctggcc tggcggcagg cttcttcgag cacccacgcg ccgacgggga ttatgagacc
     4321 tgactcttcc agcacaggga tgaagtcgga cggagatacc aggccacgct cgtggttatg
     4381 ccaacgcaag agcgcctccg cgccgacgat gtcgcccgtc gcgagatcga cctgaggctg
     4441 gtagtagaga aggaactcct ccctatccag cgcgtgcctg agatcgcgct caagtagcag
     4501 gcgctcagac gcctgaacgt tcatctcctc cgtatagaac tggtggtcgt tcccgccttc
     4561 tcctttggcg cggtacatcg cggcgtcggc gtcctttagt aacctgtcgc ccatggaggg
     4621 cgggctcacg gcgatgccga tgctaacgcc gatcggtatc tcgtggccgt tgacgacgaa
     4681 aggctcggag ataatatcta tgaggtcctc cgcgacccgc gcggcctccc ttgcttcgac
     4741 aaggtcctcg atgatgatcg cgaactcgtc gcctcccagg cgggcgacgg tgtcgctctc
     4801 acgcacccgc gcgtcgatac gccgcgctac ctgagtcagc aacgcgtccc cgccgatgtg
     4861 ccccacggtg tcgttcacag ccttgaaccg gtcgaggtcg aggaagagaa gcgcgagcat
     4921 tcctccatgg cgctcgaccc tggcgaccgc gtgctccagg cggtcgtgga agagagcgcg
     4981 gttggccagg cccgtcaggg gatcaaactg cgccagtcgg gccagcttct cctcggcgat
     5041 tttgcgctct atcgagtagc gtatggagcg ggagatcagg tccccatccc cctgtccttt
     5101 gacgaggtag tcctccgcac cgccctgcaa cgcctgcagg gcgatctcct cgtcgtccct
     5161 tccgctgagg accacgagcg gaagttcagg cgcggccctc cgcatccgct cgacggtctg
     5221 gagtcccgct gaatcgggta gcgagaggtc gaggag
//
LOCUS       DATAIR010000185         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_104030, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000185 DATAIR010000000
VERSION     DATAIR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104030"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHM69_04385"
     CDS             complement(<1..405)
                     /locus_tag="VHM69_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739661.1"
                     /translation="MADLRRTAWTAAKAFEKHFDAMRFRLKQRFGLLDPFEILPYRGH
                     GTARELFLKGRVLEEAGITRAGQDDTVWHNVRNMARRFASDEVAGARVRASFGGLQVE
                     TEADVEGFFEVGFRLPEALDGPAGWYPVKLELL"
     gene            complement(426..767)
                     /locus_tag="VHM69_04390"
     CDS             complement(426..767)
                     /locus_tag="VHM69_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739662.1"
                     /translation="MVIISVRQVRFIQGMLVKQKIRGDMVDEGGAPDARGDSQAPKIS
                     DALKKGVFVSLVTWGGRELKGVICDREQTGLLLDISESADGSEGYLFLPWSSIEQVYI
                     QSVSPRRVKVL"
     gene            complement(868..1086)
                     /locus_tag="VHM69_04395"
     CDS             complement(868..1086)
                     /locus_tag="VHM69_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739663.1"
                     /translation="MADTDNVMRYDVELKDHSETNATCEGDLSFAAFSLAHAPCGDKV
                     VEVRLGEHTLLEWCTACAVMEIFGLREE"
     gene            complement(1125..2186)
                     /gene="ruvB"
                     /locus_tag="VHM69_04400"
     CDS             complement(1125..2186)
                     /gene="ruvB"
                     /locus_tag="VHM69_04400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX2739664.1"
                     /translation="MNAEEMDDFTVRGEEDITGATGFGEHPLDPEPYPEDDEPTLRPR
                     SLDEFVGQEELKQNLRIFVQAAKQRSEPLDHVLFAGPPGLGKTSLCRILAEEMGVGLR
                     PTSGPSLERAGDMAAILTALEEGDFLFIDEIHRLNRQIEEVLYPAMEDYAMDIVLGQG
                     PSARTIRMDLPRFTLVGATTRTGLMTKPLLDRFGFSARLDYYGPEDLEKIVVRNARIL
                     DIPITGSGAWQLARRSRGTPRVANRLLRRVRDYAQVVGDGNIDEETANAALEMLGVDH
                     LGLDRADRDYLGLVIDKFDGGPVGVGTISVALGEARDTVEDVYEPYLLQSGLIQRTPR
                     GRIATHRAYDHLGVPVPND"
     gene            complement(2183..2776)
                     /gene="ruvA"
                     /locus_tag="VHM69_04405"
     CDS             complement(2183..2776)
                     /gene="ruvA"
                     /locus_tag="VHM69_04405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX2739665.1"
                     /translation="MIYRLRGTLVEKDTEGVVIDVGGVGYRSSTSLATLRALPSLGEE
                     CVIHTRMVVREDAMLLFGFAGREERAAFDALTAVSKVGPKLALSVLSSMSPPEISEAV
                     ARGDVIKLSSVPGLGKKTAERLVLELKGKSLAAFGPEPVAASGEGGGGPYMEARDALT
                     ALGYRLEEAEKALNDVPPQDTVEKYIKEALRRIGSRR"
     gene            complement(2800..3321)
                     /gene="ruvC"
                     /locus_tag="VHM69_04410"
     CDS             complement(2800..3321)
                     /gene="ruvC"
                     /locus_tag="VHM69_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX2739666.1"
                     /translation="MLATGGRGHTILGIDPGTATMGWGVIRQEGNRLRYVQHGAITTP
                     SDWEMPRRLGRLFDGVTELVRGYRPETVAVEELFFNTNVTTAITVGQARGVALLATYK
                     AGIDVAEYTPLQVKQAITSYGRADKRQVQEMVKALLNLPAIPRPDDAADGLAIAICHA
                     FSSSLPGKVGVGK"
BASE COUNT          597 a         1173 c          993 g          642 t
ORIGIN      
        1 gagcaactcc agcttaacag ggtaccaccc ggccggcccg tcgagcgcct ccggcagccg
       61 gaagcccacc tcgaagaacc cctctacgtc ggcctcggtc tcgacctgca accctccaaa
      121 cgacgcccgt acccgcgcgc cggctacctc gtcgctcgcg aaccgccgcg ccatgttgcg
      181 cacgttgtgc caaacggtgt cgtcctggcc ggcacgcgtg atgccggcct cctccaggac
      241 gcggcccttc aggaacagct cccgcgccgt gccgtgtccg cggtagggga ggatctcgaa
      301 tgggtcaagc agcccgaagc gctgcttcaa cctgaaccgc attgcatcga agtgtttctc
      361 gaaggctttc gccgccgtcc acgccgtccg tctcaagtcg gccaaagaaa cccgatccag
      421 ccgacctaga ggaccttgac ccgcctcggc gatacagact gaatgtacac ctgctcgatg
      481 ctggaccatg gaaggaataa gtacccttct gatccgtctg cggactcgct gatatcgagc
      541 agtaatcctg tctgttcccg gtcgcagatc acacccttca actcccggcc gccccacgtg
      601 actagagaga cgaaaacgcc cttcttcaac gcatcggaga tcttcggggc ctgcgaatct
      661 cccctcgcgt cgggggcacc accctcgtcg accatgtcgc ctctgatctt ctgcttcacc
      721 agcatcccct ggatgaacct aacctgtcgt acggaaatta tcaccatgtg gaggcctctt
      781 caactatagg tcaagaggcg aaagtgcgcc cggtccgggt tctacacccc tagcggaagc
      841 aggtatggga gaactccgaa atccgggtta ctcctcgcgc aggccgaaga tttccatgac
      901 ggcgcacgcc gtgcaccact caagcagcgt gtgctcgccg agacgcacct ccaccacttt
      961 gtccccgcac ggcgcgtggg ccaggctgaa agcggcgaaa gagaggtctc cttcgcatgt
     1021 agcattggtc tcagagtggt ccttcagttc tacgtcgtag cgcatcacgt tatcagtatc
     1081 ggccacttca aggtcgggct gtagcgaccg gtcttccggg gctatcagtc gttcgggacg
     1141 gggacaccga gatgatcgta ggccctgtgg gttgcgatcc ggcccctggg ggtgcgttgg
     1201 atcaggccgc tctgcaacag gtacggctcg tagacgtcct cgacggtgtc gcgcgcctca
     1261 ccgagggcga cggagatcgt gccgacgccg accggcccgc cgtcgaactt gtctatgacg
     1321 agcccgaggt aatccctgtc ggccctgtcg aggccgaggt ggtccacgcc cagcatctcc
     1381 agcgcggcgt tggccgtctc ctcgtctata tttccgtcgc cgacgacctg ggcgtagtcg
     1441 cggacccgcc tgaggagtct gttggcgacc ctcggggtgc cgcggctgcg gcgcgcgagc
     1501 tgccaggcgc cggagccggt gatcgggatg tcgagaatcc tggcgttgcg gacgacgatc
     1561 ttctcgaggt cctccggtcc gtagtagtcg agcctggccg aaaacccgaa gcggtcgagc
     1621 aagggcttgg tcataagccc ggtgcgggtc gtggcgccca cgagagtaaa gcgcgggagg
     1681 tccatcctga tggtgcgcgc ggagggaccc tgcccgagca ctatgtccat cgcgtagtcc
     1741 tccatcgcgg ggtagaggac ctcttcgatc tgccggttga gacggtggat ctcatctatg
     1801 aacaggaagt caccctcctc cagggcggtc aggatggcgg ccatgtcccc ggcacgctcc
     1861 agactcggac cgctggttgg cctgagcccg acacccattt cctcggcgag gatgcggcag
     1921 aggcttgtct tcccgagccc cggcggaccg gcgaacaaca cgtggtccag cggctccgaa
     1981 cgttgtttgg cagcctgaac gaagatcctg aggttctgtt tcagttcttc ctgcccgacg
     2041 aactcgtcga gcgagcgcgg cctgagcgtc ggctcgtcgt cctcgggata gggctctggg
     2101 tcgagcggat gctctccgaa acccgttgcc cctgttatgt cctcctcgcc acggacggtg
     2161 aaatcgtcca tctcctcggc attcaacgcc tgctccctat ccgacgtaac gcctctttga
     2221 tgtacttctc gaccgtatcc tgcggcggca cgtcgttcag cgccttctca gcctcctcga
     2281 gcctgtaccc gagcgcggtg agcgcgtccc gcgcctccat gtaaggaccg cccccgcctt
     2341 caccactcgc ggcgaccggt tccggcccga aggccgcgag gctcttgccc ttaagctcca
     2401 aaacgaggcg ctccgccgtc ttcttcccta accctggcac ggaggagagc ttgatcacat
     2461 cccccctcgc tacggcctcg gagatctccg gcggcgacat ggaggaaagg acggaaaggg
     2521 ccagcttcgg cccgaccttg ctcaccgctg tcagcgcgtc gaacgcagcc cgctcctcac
     2581 gtcccgcgaa cccgaagagg agcatcgcgt cctcccgcac caccatcctg gtgtgaataa
     2641 cgcactcctc gccgagcgaa ggcagggccc gcagcgtagc gagcgaggtc gaggacctgt
     2701 agcccacgcc ccccacgtcg ataacgacgc cttcagtatc tttctcgacc agcgtaccgc
     2761 gcagcctgta tatcatgggc taattatccc gcaccttcgc tatttcccca ccccaacctt
     2821 gccaggcaga ctactggaga aagcgtggca gatggcgatt gccaggccgt cggcggcgtc
     2881 gtcgggacgt ggtatcgcag gcaggttcaa cagcgccttg accatttcct gcacctggcg
     2941 cttgtcggcc cttccatacg aggtgatcgc ctgcttcacc tgcaacggcg tgtactcggc
     3001 cacatcgata ccggccttgt aagtcgcgag tagcgcgaca ccccgcgcct gcccgacggt
     3061 gatcgccgtg gtcacgttgg tgttgaagaa gagctcctcg accgcgacag tctcgggcct
     3121 gtagcctcga accagctcgg ttacgccgtc gaagaggcgc ccgagcctgc ggggcatctc
     3181 ccaatccgaa ggcgtggtga tggccccgtg ctgcacgtag cgaaggcggt tgccctcctg
     3241 acggatcaca ccccacccca tcgtcgccgt ccccgggtct atgccgagta tggtatgtcc
     3301 cctgccaccg gtcgcgagca tggactaaga ttaccccctc ccgccacacc ttgcaccggc
     3361 ttctacatct tgtaggttcg atagatctct ctactacgcc aaaca
//
LOCUS       DATAIR010000186         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_11893, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000186 DATAIR010000000
VERSION     DATAIR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11893"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VHM69_04415"
     CDS             <1..378
                     /locus_tag="VHM69_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX2739667.1"
                     /translation="AFLRASTALADKKEVSADDAAGAIEAALGGVKQRGKAEVGDKTI
                     VDALEPAARDAKEAAGEGSVAGVFGAAARAAKEGAESTVPLTARRGRASYLGARAQGH
                     KDPGATSTYLLLDAAARSLEGAS"
     gene            378..773
                     /gene="dhaM"
                     /locus_tag="VHM69_04420"
     CDS             378..773
                     /gene="dhaM"
                     /locus_tag="VHM69_04420"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEX2739668.1"
                     /translation="MVGIVLVSHSPKIAEGTADLVLQMAGEVEISAVGGDAEGGFGTD
                     SERIEAAIRDIGADEVLVFMDLGSAVLSAETVLEMLPPEEREKVTLVDAPFVEGAFAA
                     GVIASTGSDAEECVEAAMEARTEPKLQEG"
     gene            792..1463
                     /locus_tag="VHM69_04425"
     CDS             792..1463
                     /locus_tag="VHM69_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2739669.1"
                     /translation="MAVSQEREDPWGEVWGSLSEPWRACVELAWEAYRAGSLPIGAVV
                     ADAQGNVLSRGRNRIYERSGEDGYLFGHKLAHAEINALITLDYDRSDPCVLYTTTEPC
                     PLCVGAMRMSDLRELRYAAREPWGGSAAMFETVPYLKRGNVRVVGPEDGRLEEILVAM
                     QIERFLRLKPEVLERFLGTYEAVIPDAVLAGRNLYRSGTLKEMSVVEVPAVLRVVSQE
                     LRAVA"
     gene            1517..>2830
                     /locus_tag="VHM69_04430"
     CDS             1517..>2830
                     /locus_tag="VHM69_04430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2739670.1"
                     /translation="MTRQIPEDIGDYRFDMIVIGAGINGAGIARDAAMRGLNVLLLDK
                     GDISAGTTQWATRLIHGGLRYLEHYEVHLVRESLAEREKLLHIAPHLVKPLRFVVPIY
                     ERSKRGPALIRLGMVGYDTLSFDKSVPNHRMLSREEALEQYPGLNPEGLLGAATYYDG
                     QVEYAERVAVENAISAREHGATVLTYAEVKRLLFEENEGLTRVSGVEFEDRLGSGTYT
                     AHAPVTVNVAGPWVDRVLSGAVETTESDQMIGGTKGSHIIVDPFPGAPRDALYVEART
                     DGRPYFIVPWNGRYLIGTTDFRYRDDLDYVSADDQEISYLLEETDAVIPEANLGLDDV
                     LFTYSGVRPLPFKPEGSESSITRSHVVYDHATGKSAAGGRLTVEGAGPKADGLISIVG
                     GKLTTYRNLGRQTVDVAYKKLGREAPKSRTDKVPLPGGATQDFAAF"
BASE COUNT          513 a          875 c          982 g          460 t
ORIGIN      
        1 gccttcctcc gcgcctcgac ggcacttgcg gataagaagg aagtctccgc cgatgacgcc
       61 gcaggagcga tcgaggctgc cctcggcggc gtgaagcagc gcggaaaagc cgaggtgggg
      121 gacaagacga tcgtcgacgc cctggagccc gcggcacggg acgcgaagga ggccgcaggc
      181 gaagggagcg tggccggggt gttcggggcc gcggcgcggg ccgcgaagga gggggccgag
      241 tcgactgtcc cgctcacggc gagaaggggg agggcgagct atctcggcgc cagggcccag
      301 ggacataagg accctggggc gacatcaacg tatctccttc tggatgcggc ggcgcggtcc
      361 ctcgaaggag cctcctgatg gtgggaatcg tgctggtctc gcacagcccc aagatcgccg
      421 aggggacggc tgatctggtc ttgcagatgg caggggaggt cgagatctcg gccgttgggg
      481 gcgacgccga aggagggttc ggcacggact cggagaggat cgaggccgcc atacgcgaca
      541 tcggcgccga cgaggtgctc gtgttcatgg acttgggcag cgccgtcctc tccgccgaga
      601 ccgtgctcga gatgcttccc ccggaggagc gagagaaggt cacgctcgtg gacgctccct
      661 tcgtcgaagg agccttcgcc gccggggtca tcgcctccac aggctcagac gcggaggagt
      721 gcgtggaggc ggcgatggag gctcgcacgg agccgaagtt gcaggagggg taggggaggc
      781 cagcggcccg catggctgta tctcaggaac gagaggaccc gtggggcgag gtgtggggga
      841 gtctctccga gccgtggcgc gcttgcgtgg agcttgcctg ggaagcgtat cgtgcgggct
      901 cgctgccgat cggggccgtc gtggcggatg cgcaaggcaa cgtcctctcg cggggcagga
      961 accgcatcta tgaaaggtca ggcgaggatg gctacctgtt cgggcacaag cttgcccacg
     1021 ccgagatcaa cgccctgatc acgctggact acgaccggtc cgatccctgc gtcctttaca
     1081 ccactacgga accgtgcccg ctctgcgtgg gtgcgatgcg gatgtctgat ttgagggaat
     1141 tgcgatacgc cgcgagggag ccctggggcg gctctgccgc gatgttcgag acggtgccct
     1201 acctcaaacg cggcaacgtc agggtagtcg gtcccgagga cggacgtctg gaggagatcc
     1261 tggtcgctat gcagatagag cgtttcctgc gcctgaagcc cgaggtcctc gaacgctttc
     1321 tcgggactta cgaggcggtt atacctgatg cggttctggc gggccggaat ctataccgct
     1381 ctggtaccct gaaagagatg agcgtggttg aggtcccggc cgtgctacgg gtcgtgagcc
     1441 aggagctgcg ggccgttgcg taattacata gctgttctga cggattcgtc aggacgcctg
     1501 gatggaagga gctgtcgtga cccgtcagat tcctgaagac ataggggatt accggttcga
     1561 catgatcgtg atcggggccg gcatcaacgg cgccgggata gcgagggacg ccgccatgcg
     1621 cggcctgaac gtattgctcc tcgacaaggg cgatatctcg gcgggcacga cccagtgggc
     1681 gacgcgtctc atccacggcg gactgcgcta cctggagcac tacgaggtcc atctcgtgcg
     1741 cgagtcgctc gccgagcgcg agaagctcct gcacatcgcg ccgcacctcg taaaacccct
     1801 tcgcttcgtc gtcccgatct acgagaggag caagcgcggt ccggcactga tacggctagg
     1861 catggtcggc tacgatacgc tctctttcga caagtcggtc ccgaaccaca ggatgctctc
     1921 ccgcgaagag gccctcgaac agtaccctgg cctgaaccca gaaggcctgc tcggcgccgc
     1981 cacctattac gacggccagg tcgagtacgc ggagagagtt gccgtcgaga acgcgatctc
     2041 cgcccgcgag cacggcgcga ccgtcctgac ctacgccgag gtgaaacgcc tgctcttcga
     2101 ggagaacgaa ggtcttaccc gcgtgagcgg cgtcgagttc gaggaccgac taggtagtgg
     2161 gacatacacc gcccacgctc cagtgaccgt caacgtggcg gggccctggg tcgacagggt
     2221 gctctccggt gccgtcgaaa ccacggaatc ggaccagatg atcgggggca ccaaagggtc
     2281 gcacatcata gtagatccgt tccccggcgc gccgcgtgac gcgctctacg tcgaggcccg
     2341 cacggacggg cggccttact tcatcgtccc gtggaacggc cgctacctca tcggcaccac
     2401 cgacttccgc tacagggacg acctcgacta cgtctccgcc gacgaccaag agatctccta
     2461 tctcctcgaa gagaccgacg ccgtgatccc cgaggccaac cttggcctcg acgacgtgct
     2521 tttcacgtac tcgggcgtga ggccgcttcc tttcaaaccg gagggttcgg agagctccat
     2581 aactcgcagc cacgtcgtct acgaccacgc gacgggcaag tcggcggcgg gtggccgcct
     2641 caccgtcgag ggggcgggac ctaaggctga cggtctcatc tccatcgtcg gcggcaagct
     2701 gaccacgtac cgtaacctcg gccgccagac ggtggacgtc gcgtacaaga agcttggccg
     2761 ggaggccccg aagagccgta cggacaaggt tccgctgcca ggtggcgcca cgcaggactt
     2821 cgccgccttc
//
LOCUS       DATAIR010000187         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_289101, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000187 DATAIR010000000
VERSION     DATAIR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289101"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..613)
                     /locus_tag="VHM69_04435"
     CDS             complement(320..613)
                     /locus_tag="VHM69_04435"
                     /EC_number="4.2.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF005475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate lyase"
                     /protein_id="HEX2739671.1"
                     /translation="MKPPEECASIEDVRHGIDALDREVIALLGRRARYVEVAAQFKTG
                     ESSVRAPERQRTMLEARRRWADEEGLSPDVIEKLYRTLISHFIDRELGHWRRA"
     gene            complement(610..1578)
                     /locus_tag="VHM69_04440"
     CDS             complement(610..1578)
                     /locus_tag="VHM69_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739672.1"
                     /translation="MRDLRVSQDAARPNDHSGLSALREAAAVVDRSRRILHLTGRDPI
                     GMLNAILTNDVPTQEDRGAYAMLLNPKGRIQTDLRVIKAGEGILIDTEPEGSEAAKEI
                     LGRYAPFSRVKFEDIPESWSVLGLYGPRAGELLGDLGLAEHQSKEIEIEGRTLLVVGV
                     EVPIGGFDLIGPPKSLAAARGHLIETGATQADSATYETARIEAGVPRFGADITPENFP
                     GETGVIDRAVSFEKGCYPGQETVARMHYRGHPNKSLYHLTIEGAEVVAGAKILQGEKV
                     VGTITSVAPIPVEDRAPALGYLSRNADTGGSLRAGEARVRVLGAVS"
     gene            1610..2251
                     /locus_tag="VHM69_04445"
     CDS             1610..2251
                     /locus_tag="VHM69_04445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2739673.1"
                     /translation="MVKTIVLDVDGTLMDTNYLHTEAWARAFERVGHRVPRFRLHKQI
                     GKGAELLIREFVEDEETLQKIQDLHSEIYGELQEYGHPLPGAKELISSLKERNYGVWF
                     VTSAKDEELEHHMQELEAEGIIDGVVNSSAVENSKPAPDIFYEALKRAGATADESVAV
                     GDAVWDVEAARGAGIRTVAVLSGGAYHRDDLEEAGAVAVYEDCAALLDSDFPE"
BASE COUNT          440 a          666 c          716 g          464 t
ORIGIN      
        1 ctacttgaag attttctatc ttgagcatca aacccctgcc gttatataaa gaactttgct
       61 acagtagagc tattaaatca cagcaacata cccctaacaa cgtaaagaga tcacgaccag
      121 gaatgcctcc tgaaatccac gaaatccgtc tctttatgca gatttcgtga cctggatcac
      181 accacccgga tacctcttgg gagtcgttgt cagctcactc cactaatgtc cgcttgtaac
      241 agccgcagag taccatgacc ggctcttatt gagaatgatt cccgctaacg cgcaggcatc
      301 cggatgcagc tcgttgttgc taagctctgc gccagtggcc cagttcgcgg tcaatgaagt
      361 gagagatcaa agttctgtag agtttttcga tcacgtcagg gctcagcccc tcctcgtcgg
      421 cccatcggcg gcgtgcttcg agcatcgttc tctgtcgttc gggggcgcgg acgctcgatt
      481 cgccggtttt gaactgcgct gccacctcga cgtagcgggc gcggcgtccg agcagggcga
      541 tgacctcgcg gtccagcgcg tcaatgccgt gtcgcacgtc ctcgatgctc gcgcactcct
      601 ccggtggttt caagagaccg ccccgagaac acggaccctg gcttctccgg ctctaagaga
      661 tcctccggta tccgcattgc gggaaaggta gccgagcgcg ggtgcccgat cttccacggg
      721 tattggggcg acgctcgtga tggtgccgac gaccttctcg ccctggagga tcttcgcgcc
      781 ggctacgacc tccgctccct cgatagttag gtggtacagg gacttgttgg gatggccgcg
      841 atagtgcatg cgggctaccg tctcctgtcc agggtagcat cccttctcga agctcacggc
      901 gcggtcgatg actcctgtct cgccggggaa gttctcaggg gtgatgtcgg ctccgaagcg
      961 gggaacgccg gcttcgatgc gggccgtctc gtaggtggcg gaatcggcct gggtcgcgcc
     1021 ggtttcgatc aggtgcccgc gggccgcggc gagggatttt ggtggtccga tcaggtcaaa
     1081 tcccccgatg ggaacctcca cgccaacgac gagcagggtc cggccttcga tctctatctc
     1141 tttggattgg tgttcagcga gcccgaggtc cccgagcaat tcaccggcgc gtggaccgta
     1201 caggccgagc acggaccacg actccgggat gtcttcgaac ttcacgcggg agaaaggagc
     1261 gtagcggccc aggatctcct tcgccgcctc cgatccttcc ggttcggtgt cgatcagaat
     1321 accttcgccg gccttgataa cgcgcaggtc ggtctggatg cggcctttgg gattgaggag
     1381 catggcgtag gcgccgcggt cttcctgcgt tggcacgtcg ttcgtcagga ttgcgttcag
     1441 cataccaatg gggtccctgc ctgtgagatg caggatcctt cgcgagcggt ccacgacggc
     1501 ggcggcttct cgtagcgctg aaaggccaga gtggtcgttt ggtcttgcgg cgtcctggga
     1561 aactctcaga tctctcatag gattattcta aggcacgaga ggagctttca tggtgaagac
     1621 aattgtactc gacgtggacg ggacgctgat ggacacgaac tacctgcaca cggaggcgtg
     1681 ggcgcgggcg ttcgagaggg tgggccatcg ggtgccgagg ttcaggctcc acaagcagat
     1741 tggtaagggc gcagaacttt tgatccgcga gttcgtcgaa gacgaggaga ccctgcagaa
     1801 gatccaggac ctccacagcg agatctacgg ggagctgcaa gagtacggac atccgcttcc
     1861 gggggcgaag gagttgatct cctccctcaa ggagcggaac tacggggtct ggttcgtgac
     1921 cagcgcaaag gacgaagaac tggaacatca tatgcaggag ctcgaagcgg agggcatcat
     1981 agacggcgta gtcaactcca gcgccgtcga gaactccaag ccagcccccg acattttcta
     2041 tgaggccctc aaaagagccg gagccacggc ggacgaaagc gtcgccgtcg gtgacgcggt
     2101 ctgggacgtc gaggctgcca ggggagccgg tatacgcacc gtcgccgtcc tctccggcgg
     2161 cgcctaccac agagacgacc tcgaagaggc cggggcggtg gcagtctacg aagattgcgc
     2221 cgcactcctc gactccgact tccctgagta gcgccgggtt cgcgtgaaag cgggtttggg
     2281 aagggt
//
LOCUS       DATAIR010000188         6555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_51524, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000188 DATAIR010000000
VERSION     DATAIR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51524"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1074
                     /locus_tag="VHM69_04450"
     CDS             112..1074
                     /locus_tag="VHM69_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2739674.1"
                     /translation="MVLFRQLECFLAVERLGNLSRAAEEMFLTQPTLTARVKALEEEL
                     GDALFVRTSRGMRLTEVGREFLPYAERIVGSFEEGKRRLAELRGASGGRLRIGASPGV
                     GTYALPGLLERFAAAHPRVSISVRTGHSEAILEMTLKEEVQLGLTRAMRHPEIESLPL
                     YEDELVLIVDPGHRFTRRGIASLTEIGKEQLIFFDHDSSYFEQTHALFRNAGIGELRT
                     MEVDNIEAAKRMVEHRLGVAFLPRTAVVRSVSTGNLVLVSIEENPEMSRSIVALKRRD
                     VPSTGPVAAFLEVADSMGEKRDSTQLSPALATEFENLQLIDLFT"
     gene            complement(1182..2060)
                     /locus_tag="VHM69_04455"
     CDS             complement(1182..2060)
                     /locus_tag="VHM69_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739675.1"
                     /translation="MSKRLHESKVMVNVKRRIPIIAIVAAFAMLIASGCNANDGANAG
                     SDQSQGGAGSSSARDLPQGAEPVDLDPKDFTTRIDNPYWPMDPGTRWTYRETDEEGAE
                     LKVVVTVSNQTKKIANDVTARVVRDTVTEDGELIEDTFDWYAQDREGNIWYLGENTAE
                     FENGKLVTKEGSFEAGVDGAQPGIIMPADPRDGMQYRQEYYKGEAEDNGEVLSTDEMA
                     QVPFGQYDNALLTKDTITIEPNVLEYKLYARDVGPVLTLGVSGGPGSREELINLDTAS
                     QKETEEAGSTPLGSAS"
     gene            complement(2241..3632)
                     /locus_tag="VHM69_04460"
     CDS             complement(2241..3632)
                     /locus_tag="VHM69_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2739676.1"
                     /translation="MPIRWRLTLYIALVIGAILLALGLALYFLSRDVLLSGVEDTARS
                     RATAAARTIESGESLSDAYVEQLSLDGVVVIVRDGDGGVLLKVNIPAKGDADSAVWRE
                     AIGSGEPASGTAMLSGDDPYYVYAVPVDPPEGPARVVEAAKRYEPAQDALEVFGTVLV
                     AGIGAAILLSIGGAYLLAGAALRPVEAVTRAAQEMGEGELSKRLPVANPGDEVGRLAA
                     TINGLLSRLEAAFARREEALERQRRFAADASHELRTPLTAISGHARMLDEWALEEDPQ
                     RAKRSVRAIRAAAARMRTLVESLLILTRGDESTSLDVGRNDLASVAEEATQAARTADE
                     GKVSIEYVRPKRPVEATFDRDRVFQVATILLDNAVKYTNEGGSVLVRVEEEDGGSVLE
                     VSDTGVGIPEDELPLVFERFYRTDRSRTDSGVGLGLSIARQIAEAHGGEIRARSAPGK
                     GSTFTLHLPQPDP"
     gene            complement(3638..4315)
                     /locus_tag="VHM69_04465"
     CDS             complement(3638..4315)
                     /locus_tag="VHM69_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739677.1"
                     /translation="MRAGTRVLLVEDDASIVSFVEPELERVGFHVRSAYDGVSGLDEA
                     RRFGPDIVVLDIMLPGLDGVGVLKRLRSEGSRVPIVMLTARDATIDKVHSLDLGADDY
                     LTKPFDIDELLARMRALLRRVEGDEILRVADLEVNTSTREVRRGEREIDLTAREYDLL
                     EFMAVNPRRVLSRDLLLSRVWDREFGVTANAVEVYIAYLRKKVDAPGERKLIHTIRGA
                     GYALKGT"
     gene            complement(4438..4776)
                     /locus_tag="VHM69_04470"
     CDS             complement(4438..4776)
                     /locus_tag="VHM69_04470"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX2739678.1"
                     /translation="MVDAKKRKVLLAALAAAVMLLVAGVAYASGDDSSEKQATGPGIE
                     KAKSVALDHTNGGRVSATEVGDEEGYYEVEVIRDDGSQVDVHLDPDFNVLGAQADRES
                     PDDKDAPNDD"
     gene            complement(4927..5160)
                     /locus_tag="VHM69_04475"
     CDS             complement(4927..5160)
                     /locus_tag="VHM69_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739679.1"
                     /translation="MEVFLTIVAIVVVGVVVGLVFGFRGRGGGDGGSGAASGYYGTFG
                     DSEHHGDGGGGWFGGGGGDGGGGGDGGGGGGGN"
     gene            5331..6407
                     /locus_tag="VHM69_04480"
     CDS             5331..6407
                     /locus_tag="VHM69_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2739680.1"
                     /translation="MDPKTIVVMEGDQTGQELLEEALRVLDPAVTGLDLDFTRYDLSL
                     ENRRATGNEVVHQAAAAMKESGFGIKAATVTPEDPDDVGSPNAILRKGIDGTVIVRTG
                     RRIPGVNPTSGVHAPISVVRMAVDDAYGAREWREGEGQDEVAYRTEKISRRVCRGVSE
                     FAFIQARKMRAKVFGGPKWTVSPVYEGMFKEEMDRAAANSRDVRYDPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDEEENPETVMAEAPHGTA
                     PSLQGKNVANPMAMILAGAALLTFFHEEESSRVSRAIYEATFETVLDGVRTADLGGSA
                     GTTEFTDEIIRHVRTKLEVWSALA"
BASE COUNT         1185 a         2115 c         2010 g         1245 t
ORIGIN      
        1 atagtttctc ctctcaactt cttcgtcgtg aaagcaagat accaacaccc ttgatattta
       61 tccaatcgtt tgcttgaatt atctcaatag agaaaatcta tatcggagga tatggtgctc
      121 tttcggcagt tggaatgctt cctggcggtg gagagattag ggaacctgag ccgggctgct
      181 gaggagatgt tcctgacgca accgacgttg acggcgcgag taaaagcgct agaggaggag
      241 cttggggacg cgctcttcgt gcgcacgagc cgtgggatga ggctaacgga ggttggcagg
      301 gagtttctac cttatgcgga gaggatcgtc ggcagtttcg aggaaggcaa gcgccggttg
      361 gccgagctca ggggggcttc cggggggcgg ctcaggatcg gggcttcgcc cggtgttggg
      421 acctatgcgt taccagggct attggagagg ttcgcggcag cgcatccgag ggtttcgatc
      481 tccgtaagga ccgggcattc cgaggcgatc ctggagatga cactcaagga agaggtgcaa
      541 ctcggcctca cgcgggctat gcgccatccc gagatagaaa gtctgcccct ctacgaggat
      601 gagctggtgc tgatcgtgga tcctggacac aggttcacca ggagaggaat tgcgagtctg
      661 acggagatcg ggaaggagca actcatcttc ttcgaccacg actcgagtta cttcgagcag
      721 acacatgcgc tgttcaggaa cgccggcatc ggggagctca ggaccatgga ggtcgacaac
      781 atcgaggccg ccaagaggat ggtcgagcac cgcctcggag tggcgttttt gccccggaca
      841 gccgtggtca ggtcggtctc gaccgggaac ctcgtcctcg tctcgataga ggagaacccc
      901 gagatgagcc gttcgatagt cgccctcaaa cgtagagatg ttccgtcgac gggcccagta
      961 gccgccttcc tcgaagtcgc ggactctatg ggcgagaagc gcgacagcac ccagctctcc
     1021 cccgccctcg cgaccgagtt cgagaacctg cagctcatag accttttcac ctgatggaga
     1081 accgccagag ttcagcaggt acgtacggtc tgtagaccac gcaggagcgc cgagtccttc
     1141 tcacctgccg ggcttccccg ggtacccatc gtccgtcacg cttacgaagc ggaacccagc
     1201 ggtgtcgaac cggcctcttc cgtctccttc tggctggccg tgtcgaggtt gatgagctcc
     1261 tcgcggctgc ctggaccgcc cgagactcca agggtgagca caggtccgac gtccctggcg
     1321 tacagcttat actcgagtac gttcggctcg atcgtgatcg tgtccttcgt caggagcgcg
     1381 ttgtcgtact gtccgaaggg gacctgggcc atctcgtcgg tgcttaagac ctcgccgttg
     1441 tcctcggcct cacccttgta gtattcctgt cggtactgca tcccgtccct gggatcagcc
     1501 ggcatgatga tgcctggctg cgcgccgtcg accccggcct cgaaggagcc ttccttcgtt
     1561 acgagcttgc cgttctcgaa ctcggcggtg ttctcgccga ggtaccagat gttgccctcc
     1621 ctgtcctggg cgtaccagtc gaaggtgtct tcgatcagct ctccgtcttc cgtaacagtg
     1681 tcgcgcacga ccctggcggt gacatcgttc gcgatcttct tcgtctggtt gctaaccgtt
     1741 acgacgacct ttagctcggc gccctcctcg tcggtctcgc ggtaggtcca tcgcgtgccc
     1801 gggtccatcg gccagtaggg gttgtcgatc ctggttgtga agtccttcgg gtcgaggtcc
     1861 acgggttcgg ccccctgcgg caggtctctc gcactgctgg atcccgcacc gccctgcgac
     1921 tgatccgagc cggcattcgc accgtcattc gcgttgcaac cgctggcgat gagcatggcg
     1981 aacgcggcca cgatggcgat gattgggatg cggcgcttga cgttgaccat cactttcgac
     2041 tcgtggagcc ttttgctcat ccacttcctc cgtccgtcgt gtgctttatg gcgcagtatc
     2101 ccgtcgaaac ctgagaagcg gctgaggatg acgccgggac tgcataagtg atggccaggc
     2161 acacggcgaa ccgggatctt ggatccgccc gggatcgaaa cgttcgagta cctgcttcgg
     2221 cgagcgtcgg ggcgtagcgc tcaggggtct ggctgcggga ggtgaagcgt gaacgtcgaa
     2281 cccttccctg gtgcgctccg ggcccgtatc tcgccgccgt gggcttcggc gatctggcgc
     2341 gctatcgaga gccccaggcc gacgccgcta tccgtgcggg agcggtcagt ccggtaaaaa
     2401 cgctcgaaga cgagtggcag ctcatcctcg gggataccga cgcccgtgtc cgaaacctcc
     2461 agcacgctcc caccgtcttc ctcctcgact ctaaccagga cgctcccgcc ttcattggtg
     2521 tacttcacag cgttgtccag aagaatggtg gctacctgga agacgcggtc cctatcgaag
     2581 gtggcctcga cggggcgctt cgggcggacg tactcgatgg agaccttccc ttcgtcggcg
     2641 gtccttgccg cctgcgtggc ctcttccgcg accgaggcaa ggtcgttgcg tccgacgtcg
     2701 aggctcgtgc tctcgtcccc gcgcgtcagg atcaggagcg actcgaccag ggtacgcatc
     2761 ctcgcggccg ccgcgcggat ggcccggacg ctccgcttcg ccctctgggg gtcctcttcg
     2821 agcgcccatt cgtccagcat cctggcgtgg ccgcttattg cggtaagcgg cgtacgcagc
     2881 tcgtggctgg cgtcggcggc gaagcgccgc tggcgttcca gagcctcctc gcggcgcgcg
     2941 aacgcggctt cgagacgaga gaggaggccg ttgatcgtgg ctgccagacg accgacttcg
     3001 tcaccggggt tcgcaacggg cagacgcttc gacagctcgc cttcgcccat ttcttgtgcg
     3061 gctctcgtca cggcctcgac gggcctgagg gcagcccccg ccagcaaata ggcgccaccg
     3121 atggaaagta agatagccgc accgatgccg gccacaagca ccgtcccgaa gacttcgagg
     3181 gcatcctggg caggctcgta acgtttggcg gcctcgacca cccgcgccgg tccctccggc
     3241 gggtccacag gcacggcgta gacgtagtag ggatcatcgc ccgagagcat tgccgtcccc
     3301 gaggccggct ctcccgagcc tatcgcctcg cgccagacgg cgctgtcggc atcacctttc
     3361 gcaggaatgt tcaccttcag taggacgccg ccgtctccat cacggacgat cacaacgacc
     3421 ccgtcgaggg aaagttgctc gacgtaggcg tcgctcaagc tctctccgga ctcgatggtc
     3481 ctggccgcgg cggtcgcgcg gctgcgggcc gtgtcttcga cgcccgagag aagcacgtcc
     3541 ctgctcagga agtagagcgc gagccccagg gccagcagta tcgcgccgat cacaagtgcg
     3601 atgtatagcg tcagccgcca ccggatcggc atgcgtgcta cgttcccttc aaggcgtatc
     3661 ccgcgcccct gatggtgtgg atgagcttac gctcccccgg tgcatcgact ttttttctga
     3721 ggtaggcgat gtagacctcg acggcgttcg ccgtgacgcc gaactccctg tcccataccc
     3781 tgctgaggag gaggtccctg gagaggaccc ggcggggatt gacggccatg aactcgagca
     3841 ggtcgtactc gcgcgcggtg aggtctatct cccgctcccc gcgccgaacc tcacgggtgg
     3901 acgtgtttac ttcaagatcg gcgaccctga gtatctcatc gccctccacg cgcctgagga
     3961 gagcacgcat acgtgcgaga agttcgtcga tatcgaacgg cttggtgaga tagtcgtcgg
     4021 cgccgaggtc caggctgtgg accttgtcga tagtcgcgtc gcgggctgtg agcatgacga
     4081 tcgggaccct gctaccctcg cttcgcagac gcttgagcac ccccaccccg tccagaccag
     4141 gcagcatgat gtccagtacc acgatgtccg gtccgaaacg tctcgcctca tcgagtccgg
     4201 agacgccgtc gtaagcgcta cgcacatgga agccgacacg ctccagctcg ggttcgacga
     4261 agctgacgat cgaggcgtca tcctcgacga gcagcacgcg cgttccagct ctcattttct
     4321 caccacctct cgcatcgata ttctaatccc cgatctcccg gcaagagaaa ggccgggaca
     4381 actgatcccg gccctcacca agtgcagaat tgcatccagg ccttcaaggc tcggatgcta
     4441 gtcgtcgttg ggcgcgtctt tgtcgtcggg gctttcgcga tcagcctgag caccgaggac
     4501 gttgaagtcg gggtcgaggt gaacgtcgac ctggcttccg tcatcacgga tgacctcgac
     4561 ctcgtagtag ccctcttcgt cgccgacctc ggtggcactc acccggcctc cgttggtgtg
     4621 gtcgagcgcg acgctctttg ccttctcgat gccgggtccc gtggcctgtt tctcagagga
     4681 gtcgtcgccg ctggcgtaag ccacgccggc caccaggagc attaccgcag cggccagcgc
     4741 cgcaaggagc actttgcgct tcttcgcatc taccacttcg caatcccctt tcttctatgt
     4801 tcgaacagtc gggcgaagga taggagacga acctgagaag agcctgagaa ccgcatgagg
     4861 acggtgccca gcacaatcat gccggcgagg ccgaagatac ggcagcgatc ctgctacagt
     4921 gcgcggctag ttgccgcccc caccaccacc gccgtctcct cctccaccac catcgcctcc
     4981 cccgccgccg aaccagcccc cgccgccgtc gccgtggtgc tcactatctc caaaagtccc
     5041 gtaataaccg ctcgccgccc cgctcccgcc gtctcccccg ccacgaccgc ggaagccaaa
     5101 aacgagacct acgaccacgc cgaccacgac tatcgccacg atagtaagga acacttccac
     5161 cccaaccctc cattcacaaa cttccggaat aaaaccctat tctcccggca agaaccccgt
     5221 cattataact tgagatgcaa gaaggcgggt ggcgggacac gaatgcgtgc aggtgaggat
     5281 cgtatactta gcaggatcat tcacagagac tattcacaga ggtgcgacga gtggacccaa
     5341 agacgatcgt tgtaatggag ggtgaccaga cgggtcagga gctgctcgag gaggccctga
     5401 gggtactcga ccctgcggta acggggctcg acctggattt cacgcgctac gacctgtcgc
     5461 tcgagaaccg gagggccacg ggcaacgagg tcgtgcacca ggcggctgcg gcgatgaagg
     5521 agtcaggctt cggcatcaaa gcggcgaccg tgacgcctga agaccctgac gacgtggggt
     5581 cgccgaacgc catcctgagg aaaggtatag acgggaccgt catcgtacgt actggacggc
     5641 gcatccccgg cgtcaacccg acgagcggcg tgcacgcccc gatctccgtg gtccgcatgg
     5701 ccgtagacga cgcctacggg gccagggagt ggcgcgaggg cgaggggcaa gacgaggtcg
     5761 cctacaggac ggagaagatc tcgcgtcgag tatgccgggg cgtctccgag ttcgcgttta
     5821 tccaggcgcg caagatgcgt gccaaggtct tcggcggtcc caagtggact gtctctccgg
     5881 tctacgaggg gatgttcaag gaggagatgg accgcgccgc cgcaaacagc cgcgacgtcc
     5941 gctacgaccc ccagctcata gacgccacct acgcattgct cctcaccacc tacggcgagc
     6001 cgctcgtcat ccctacattg aaccgcgacg gtgactgtat ctcagacctc gtgatgcaga
     6061 tgttcggctc catcgcaggg gcagagtcgc tgctcatctc cctcgacgag gaggagaacc
     6121 ccgagaccgt catggctgag gccccgcacg gtacggcgcc ttcgcttcag ggcaagaacg
     6181 tggccaaccc gatggcaatg atcctggccg gagccgccct tctgaccttc ttccacgaag
     6241 aggaatcgag ccgcgtctcg cgtgccatct acgaggcgac cttcgagacc gtcctagacg
     6301 gcgtgaggac cgcagacctc ggtggctccg ccggcacgac cgagttcacc gatgagatca
     6361 tacgccacgt gcgcaccaag ctcgaggtgt ggagcgcgct agcgtgagaa cgtccagagc
     6421 cctcggtctg ggcgcgatct caggcctgcg ctctttctcc ggccccgcct tcgttagccg
     6481 cgccgcgagc cagggatacc tgaagcttgg aggaagcccg ctcgctttcc ttggctcgaa
     6541 gcgtctctcg caggc
//
LOCUS       DATAIR010000189        12869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_12006, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000189 DATAIR010000000
VERSION     DATAIR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12006"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2505
                     /locus_tag="VHM69_04485"
     CDS             133..2505
                     /locus_tag="VHM69_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2739681.1"
                     /translation="MNQEQNGTFGPRLRRLREAAGLTQEELASRAGLTAKAVSALERG
                     ERKRPYPHTVRSLAEALQLSEQERTVLTEAVPSRDGDSPEAAVTPMSTLPSSLTPLLG
                     REREVEEIDKLLRRAAVRLLTLTGPGGIGKTRLVTEVARMADGDFPDGVAFVALAPLG
                     DAALVMPTVSQVLGLREVAGVHPIESLRQHLREKTFLLVLDNFEHVAEAAPEVVDLLG
                     SCPNLSVLVTSRAPLRVRGEREYSVSSLAVPDPTRLPEADEVARTPAAELFVERAEEA
                     SPSFELTEANAASVAAICWRLDGLPLALELAAAQTRFLGPTALLSRLDRALEAGGARD
                     LPERQRTMRSTLDWSHDLLHEPEKELFRRLSAFAGGFTLETAEDVGAAGSVEAEDVFV
                     LLGNLVEQSLVVVETTLEGRTRYRMLEPVRQYALERLRESGEADEARKRHVRHFLALA
                     EQAEPHIKGLDQATWLDRLETENDNLRAAIGWSIETGDAQSASRFGWVLSMYWVMRSR
                     HSEGRLWMEQTVARNDLPDETRAKALWALAACVYGSGDDERLMAISEEGVAVSRRVRD
                     ARAEAYLVGMGGFAALQLGDLDRATRDLEESLRLDRELGDDWAAAHILTHLAVVPFRQ
                     GNFPRVAAYAEEALELTERTGDRLAANIALYLLAQSALASGEHEQARVYFRDALVLTF
                     EVSDLTNAAYCLQGLAAVAEAQGEPSRAATLLGAAEALLEAVGTHHYAQMDHELYDRV
                     ADAARERLGEPAWTSARREGRAMSFDEVVAYVREGDEVLPKARSETPASE"
     gene            2791..5091
                     /locus_tag="VHM69_04490"
     CDS             2791..5091
                     /locus_tag="VHM69_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX2739682.1"
                     /translation="MGAVAITLVLFSVLVAAQAQGVESSYTNPVSRAAFDAFPDPAII
                     KGKDGYWYAYGTTDPVRQSFGDNAFHFLPTARSKDMVHWEYAGDAFTESTFPSWLPGE
                     PGENTFLWAPDIRYLDGKYYLYYSVAHFGETSADSLFTIGLATAPTPTGPWTDSGGSV
                     IEGEDCPATTNIDPAEFTDKDGTRYMYWGSYGAIFAAKLTPDGTRLASGTTQISYGGQ
                     AEAPYVVRRGDYYYLFISEGGCCSGALSSYEVFVGRSEKISLGGSPLGPFVDREGVSM
                     MASRRGGTLVLSANGNKWVGPGHHSMATDLSGQQWFAYHAIDKANPYLKDPWEGINRR
                     PMLIDRLDWIDGWPTVRAGAWASEDSQVAPVSSFAVGDDFNGDTSLGAEWRREGAAQN
                     GWQLANEQDAGTYVRQSAPASRPSFLVSTGQAPADFRAEADLRLPAGSSGAAGLVAAY
                     RDPANHVEAWLDAGTKALVTDVIVNGRHSSQKTPLPTGFRFDTWHNVAIEARGTAMTV
                     EVTDARLGDPLASQQLTLPGGAGGQGAVGVASRSAQAEADNVGATPLYTPVTQRVPDP
                     TVGALDTNYSDEFNDEKLDNAWSWVRKRAGREESGVFRWPTQDADLFGDRNDASVLLR
                     DAPQGAYTVETRLTMDLGINTDRSFQQAGLVAYDNDDLYTKLVHVAIGTGRRTEFAKE
                     AGGYGGMMVGPPSDTTWLRISHRIDPNNGKPEHEYRAATSRDGEHWVWGGVWTFPEGT
                     STRLGLVSMGGSGATAEFDYFRVFRP"
     gene            complement(5098..5508)
                     /locus_tag="VHM69_04495"
     CDS             complement(5098..5508)
                     /locus_tag="VHM69_04495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2739683.1"
                     /translation="MIDVSQALEPFVRQWAVLLTTYKRDGIPVGTAVNIVVEGDHAYF
                     RTWDTAWKFRRIRNNPEVEVAPCTPRGRPTGPATQARARILEGKESAHARRLLARKYP
                     FSHGIMVPLAHRLLGYRTMHLELRCPDDASRDRV"
     gene            complement(5505..5966)
                     /locus_tag="VHM69_04500"
     CDS             complement(5505..5966)
                     /locus_tag="VHM69_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739684.1"
                     /translation="MNETVDVRRPASAGWIRGGLALISVGALSVGVWALFLPASFYED
                     FPLPGRGWVSALGPYNEHLVRDVGAMNLAFGMLLALSAGLLDLRLVQVSLAAYLVYTV
                     PHFLFHLTHTRAFSVGDNLAILILLGLLVVLPVAILIHSGLHGRNRREEGQ"
     gene            complement(5963..6760)
                     /locus_tag="VHM69_04505"
     CDS             complement(5963..6760)
                     /locus_tag="VHM69_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX2739685.1"
                     /translation="MNAPRVLVTGGTGVLGQHVVERLGAAGLQSRVLSRSGKPGTIRG
                     DLRTGEGLEVAVKDVNIIIHCATSPFRKARQVDVEGTGRLLAAATRAGVSHIVYISIV
                     GIDRAPSYPYYRIKLETERVVESSPVPHTILRATQFYDLVFMAMRFLDRLPVMPIPRG
                     FLGQPIDAGEVAGHLVALSGPAGRVPDIGGPEVMTLADAARTYLQIKGRRRRIFEVPF
                     PGKTARAFREGALVCPDRAYGKIRWEDFLRSQIETQIDLHGAEGVTR"
     gene            complement(6757..7650)
                     /locus_tag="VHM69_04510"
     CDS             complement(6757..7650)
                     /locus_tag="VHM69_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX2739686.1"
                     /translation="MSRIEVFDRNRPLLFSIAYRMLGSVMEAEDVVQEAFLRWEGASE
                     DEVRTPSAYLSTVVTRLCIDRLRSARARREQYVGPWLPEPLLEEQEIAGKADLDDSLS
                     MAFLVLLESLTPVERAVFLLREVFAYDYSEIATLVGKSEANCRQVARRARQSVAARRP
                     RFESSPEQEERLMDSFLRASLSGDMEALLALLSEDVVLYSDGGGKTRAALKPIYGSDK
                     VARFLSGILRTAPPDFAVRQTRVNARPGFVGYFGDGSPQSVVTIEVAEGSITAIRLLV
                     NPEKLANVPPLEQAESGEGSG"
     gene            complement(7746..8501)
                     /locus_tag="VHM69_04515"
     CDS             complement(7746..8501)
                     /locus_tag="VHM69_04515"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX2739687.1"
                     /translation="MNPLVAQYADRLLDAGVAGACTSHGAENNLYKIGLLLEKDAANS
                     FGMEELLQDVSFEEAYNAVAFQIGNPPDKEETEGRGCIDPSRTASGLVEAGERIREVA
                     GSGGRLIFATGHPGALILYYLGLARWAEELGGEVVTVETRGRYQRGVSLDWAESVAAL
                     GDGASLFHTHDTGPMRDLLRQTGDVDLVVADHGFAGAAIASGVPTVAVMDTNDPALAV
                     VVGRGADVTVVPMDDNRPLNAYAAALDVVFGGV"
     gene            complement(8557..8919)
                     /locus_tag="VHM69_04520"
     CDS             complement(8557..8919)
                     /locus_tag="VHM69_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2739688.1"
                     /translation="MRSTGDIYSERFCPRYQRAVEIVGRRWSGAILRAMLTGASRFGE
                     IQEVIPDLSNRMLSERLREFEAEGIVERIVVPERPVRVEYRLTEKGRSLNEVVEALSR
                     WSQDWIPLEEVEESRAAV"
     gene            9017..9427
                     /locus_tag="VHM69_04525"
     CDS             9017..9427
                     /locus_tag="VHM69_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2739689.1"
                     /translation="MVDVGLLVLRVVLGVIFAAHGAQKLFGAFGGPGLQGTAGFHGQL
                     GIRPPYLMAVLAGLAEFAGGILIAVGFLTPLAAVALILTMAVAVFTVHLKNGFFSMNG
                     GYEFNLALAGMALTLLITGAGTLSLDAVLGIVLW"
     gene            9449..10087
                     /locus_tag="VHM69_04530"
     CDS             9449..10087
                     /locus_tag="VHM69_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX2739690.1"
                     /translation="MNVTIIGSGNMARGIGSRLVAGGNSVRIIDRNEERADALAGELR
                     AQDGVEVSTGSFGDRIEDDVVVLAVPYDAVESIADQYSSGLSGKVVVDITNPVDWASF
                     DRLVTPPDSSAAEEIDSLIPEAAVVKAFNTTFASTLVGGEVGGQPLDVFVASDDPDAK
                     QKISGLIEAGGLRPVDAGPLRRARQLEGLGFLHMTLQQPLGTGFGSAVKVVA"
     gene            10178..10678
                     /locus_tag="VHM69_04535"
     CDS             10178..10678
                     /locus_tag="VHM69_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2739691.1"
                     /translation="MSSASIPAQARIGHVHLRVADLDRAIRFYRDLLGFDLLMRFGDE
                     AAFLSAGGYHHHIGLNTWQSKDGEPPAPGTTGLYHFAINYPTRGDLAVAFKRLLDNGW
                     SIDGASDHGTHEAIYLRDPDSNGIELAWDRDQSQWPRKGEELTFTRRPLDFDGLLAEL
                     EGPVAR"
     gene            complement(10697..11095)
                     /locus_tag="VHM69_04540"
     CDS             complement(10697..11095)
                     /locus_tag="VHM69_04540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2739692.1"
                     /translation="MIYETKIRVRYAEIDAQGHVNNATYLSYFEVGRVEWLRATGQSY
                     REMEDRGRGLVVVEALLHYRRPAFFDDELTLRTDLTDLGKVSLRFEYEVLRDGEVLTT
                     GHTRHACVDLSTGKPVRMPQELLRLAPDTP"
     gene            complement(11092..11865)
                     /locus_tag="VHM69_04545"
     CDS             complement(11092..11865)
                     /locus_tag="VHM69_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2739693.1"
                     /translation="MDYVKLEREDGVAVLTIDRQEKLNALDQQVVEEIGQALLELEAE
                     GPRAIVVTGAGERAFIAGADIRAMSVMDPIEAKRFSEIGHAAMALLDRSPVPTIAAVN
                     GFALGGGCEVAIACDVRIAAENATFGFPEVGLGILPGMGGTQRLPRLIGPALAKELIF
                     TGRRIGAEEAREIGLVNRVVSQGEAFDTAMELAAEISANGPVAVRHAKAAANRALDVD
                     LVSGLEYEADQFALLFATEDAREGMNAFGEKRKPNFEGR"
     gene            complement(12092..12490)
                     /locus_tag="VHM69_04550"
     CDS             complement(12092..12490)
                     /locus_tag="VHM69_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2739694.1"
                     /translation="MAHTIRVVVAKVGLDGHDRGAKIIARALRDAGMEVIYTGLHQTP
                     EQVVEAAIQEDADAIGISILSGAHMTLVPRIVDLLKENESEDILVFVGGTIPKEDIAS
                     LKEIGVGEVFTPGTPTKKAVEYVEQAVPAS"
     gene            complement(12536..>12869)
                     /locus_tag="VHM69_04555"
     CDS             complement(12536..>12869)
                     /locus_tag="VHM69_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739695.1"
                     /translation="PASEDELRVSRVAEAWAAVSVSLMLISFVGIWMFEREHLISMLV
                     VSIAFFAFAEAGFRGRLVNLVSSANIGLAVVATLVIFYEFFWQLVVAAVLVVGLYVLW
                     DNLRELRR"
BASE COUNT         2374 a         4145 c         4094 g         2256 t
ORIGIN      
        1 cgtgggagga agggcgcgcg atgacgaccg aggaagccgt cgggtacgcc ctggagaagg
       61 agaccgcacg ctgacgaggt gctgctccgc gttctcagac ggaagcaaca ctacggggga
      121 cggacaagcc acatgaacca ggaacaaaac ggtactttcg gcccgcggct acggaggctg
      181 cgcgaggccg cggggcttac gcaggaggag ctggcttcga gggccgggct gacggccaag
      241 gccgtgagcg ccctcgagcg cggcgagagg aagcgcccgt acccccacac cgtgcgctcc
      301 ctggctgaag cgctgcagct ctccgagcaa gagcgcaccg tcctcaccga ggccgtaccc
      361 agccgggacg gcgactcccc cgaggccgcc gtcaccccga tgtccaccct cccttcgtcg
      421 ctcacccccc tgctgggtcg ggagagggag gtggaggaga tagacaaact gctccgccgg
      481 gcagcggtgc ggctgctgac gctcaccggc cctgggggga tagggaagac gcgcctggtc
      541 acggaggtgg cgcgaatggc ggacggcgac ttcccggacg gcgtggcctt cgtcgccctg
      601 gcgcccctgg gtgacgcggc gctggtaatg ccgaccgtct cacaagttct tggcctgagg
      661 gaagtggcgg gcgtgcaccc tatcgagtct ctccgccagc acctgcgcga gaagaccttt
      721 cttctggtgc tcgacaactt cgagcacgtg gccgaagcgg cgccagaggt ggtggatctg
      781 ctcggctcct gtccgaacct ttccgtgctc gtaaccagcc gcgcaccctt gagggtaagg
      841 ggtgagcgcg agtactccgt ctcctccctc gcggtgcccg accccacccg cctgcctgaa
      901 gcggatgaag tggcccgaac accggccgcg gagctcttcg tcgagcgggc agaggaagcc
      961 tccccttcct tcgagctcac agaggcgaac gccgcttctg tggcagcgat ctgctggcgc
     1021 ctcgacggtc tgcctctcgc cctcgagctc gccgcagccc agacgaggtt cctcggaccg
     1081 acggcgctgc tctcgaggct cgacagggct ctcgaagcag ggggagcaag ggacttgccc
     1141 gaacgccaga ggacgatgcg gagcaccctc gactggagcc acgacctgct tcacgaacca
     1201 gagaaggagc tcttcaggag gctctcggct ttcgcaggag gattcacgct cgagaccgcc
     1261 gaggatgtag gggctgccgg atcggtcgaa gccgaggacg ttttcgtact gctcgggaac
     1321 ctggtcgagc aatccttggt cgtagtggag acgaccctcg aagggagaac gcgttacagg
     1381 atgctcgaac cggttcgcca gtacgcgctg gagcggctgc gtgagagcgg cgaggccgac
     1441 gaagcgcgga agcggcacgt ccggcacttc ctcgcgctcg ccgagcaggc cgagccccat
     1501 ataaaggggt tggatcaagc cacctggctc gacaggctcg agaccgaaaa cgataacttg
     1561 agggccgcta tcgggtggtc gatcgagaca ggcgacgcgc agtcggcttc caggttcggc
     1621 tgggtgctca gcatgtactg ggttatgcgc tccagacaca gcgaaggacg actgtggatg
     1681 gagcagacgg tggcaaggaa cgatcttccg gacgaaacga gggcgaaagc tctctgggca
     1741 ttggctgcgt gtgtgtacgg atcgggcgac gacgaacggc tgatggcgat ctccgaagag
     1801 ggtgtcgccg tctccagaag ggttcgggac gcccgcgcgg aggcgtattt ggtcgggatg
     1861 ggcgggttcg ccgcgctgca gctgggcgat ctcgaccggg cgacccggga cctcgaagaa
     1921 tcgctacggt tggatcggga gctgggagac gattgggccg ccgcccatat cctgacccat
     1981 ctggcagtcg ttcccttcag gcaagggaac ttcccgcggg tggccgcgta cgcggaggag
     2041 gccctcgagc tcacggaacg gacgggcgac aggctggccg ccaacatcgc gctctacctc
     2101 ctggcgcagt cggccctcgc gtcgggtgag cacgagcagg cgcgcgtcta ctttcgagac
     2161 gccctcgtgc tcaccttcga ggtctcagac ctgacgaatg ccgcctactg cctgcaagga
     2221 ctcgccgcag tcgccgaagc acagggcgag ccgagccgag ccgccacgct gctgggcgcc
     2281 gcggaggcgc tgctcgaagc cgtggggaca caccactatg cccagatgga ccacgagctc
     2341 tacgaccgcg tggccgacgc cgcgcgtgag cgactcgggg agccggcctg gacttcggcg
     2401 cgacgcgaag gacgcgcgat gtccttcgat gaggtggtgg cgtacgtgcg cgagggtgac
     2461 gaagtccttc cgaaagcgag atccgagaca cccgcctcag agtaaccgaa cgcgattcgt
     2521 tgacgcgttc gagattcgtg gggttcttat ctcggcagtc gtcacgacga tcacccctct
     2581 cgttccaggg tttgacatga tcgctgtgcc tccctttcag gctacttgac ggcgcttgtg
     2641 gagtcccccg gtcaacagta ataccttcct cgaaaccggt ttgctcatct tcattttcct
     2701 tcaaggccat gctataaagg tcataagggg tctctcggag cgggtggaag gataggcggg
     2761 gtggagaatc acggcttggc gtatcgatgg atgggtgctg tcgcaatcac cctggttcta
     2821 ttctcggttc tcgtcgccgc gcaggcgcaa ggagtagaga gttcttacac caacccggtg
     2881 tcccgcgccg ctttcgacgc tttccccgac cccgccatca tcaagggcaa ggacggctac
     2941 tggtacgcat acggcacaac cgaccctgtg cgccagagtt tcggcgacaa tgcgtttcac
     3001 tttctgccca cggcgagatc caaggacatg gtccactggg agtacgcggg cgacgcgttt
     3061 accgagtcaa cctttccgag ctggctaccc ggcgagcccg gcgagaatac gttcctctgg
     3121 gccccggata ttcgctacct ggacggcaag tactacctct actactccgt cgcccacttc
     3181 ggcgaaacga gcgcggatag cctcttcacg atagggctcg ctacggcgcc aacccccacc
     3241 ggaccgtgga ccgactcggg cggctcggtc atagagggtg aagactgtcc agctaccact
     3301 aacatagacc ccgcggagtt cacggataaa gacggcaccc gctacatgta ctggggcagc
     3361 tacggtgcga tcttcgcggc gaagctcaca cctgatggta cacgccttgc tagcgggacg
     3421 acgcagatct cctacggagg ccaggcggag gccccatacg ttgttcgccg cggagactac
     3481 tattacctgt tcatctccga gggcggctgc tgctccggtg ctctgagcag ctacgaggtc
     3541 ttcgtcggcc gatcggagaa gatctcgctc ggggggagcc cgctcgggcc gttcgtggat
     3601 cgggagggcg tctcgatgat ggcctcgcgc aggggaggta ccctggtgct ctcagccaac
     3661 ggcaacaagt gggtcggtcc gggccaccat tccatggcca ccgacctctc gggccagcag
     3721 tggttcgcct accacgccat cgacaaggcc aacccttacc ttaaggatcc gtgggagggc
     3781 atcaaccgca ggcccatgct catcgaccgg ctcgattgga tcgacggctg gcccacggtc
     3841 cgggccggcg cctgggcctc cgaggacagc caggtagccc ccgtcagctc cttcgccgtc
     3901 ggcgacgact tcaacggcga tacgagcctc ggcgccgagt ggcgccgcga aggagcggcc
     3961 cagaacggct ggcaactcgc gaacgaacag gacgcaggga cctacgtgcg ccagagcgct
     4021 cctgcaagca ggccgtcctt cctggtgagc accgggcagg cgcccgcgga cttcagggcg
     4081 gaggccgacc tgcgactgcc tgcagggtct tcgggagccg ccgggctcgt cgcagcctac
     4141 cgcgaccctg ccaatcacgt cgaagcctgg ctcgacgccg gtacgaaggc gctggtgacg
     4201 gatgtgatcg tgaacggacg ccacagctct cagaagacgc cgctgcccac cgggttccgt
     4261 ttcgacacct ggcacaacgt cgcaatagag gctcgcggga cggcaatgac agtcgaagta
     4321 acggacgcgc gcctgggtga tccactcgcg agccagcaac tcacgttgcc gggcggagcc
     4381 ggtgggcagg gagccgtcgg tgtagcgtcg cgatccgccc aggcagaggc ggacaacgtg
     4441 ggcgcgaccc cgctgtacac gccagtcacc cagagggtgc cggatcccac ggtcggagca
     4501 ctggacacga actacagcga cgaattcaac gatgagaaac tcgacaacgc atggtcgtgg
     4561 gtgagaaaac gagcaggccg ggaggagtcc ggcgtgttcc gctggccgac acaggacgct
     4621 gacctcttcg gagaccgtaa cgatgcctcg gtgttgctga gggacgcgcc ccagggtgcg
     4681 tacacggtcg agaccaggtt gactatggac ctcggcataa acacggaccg cagcttccag
     4741 caagcgggtc tggtagctta cgacaacgac gacctttaca ccaagctggt ccacgttgcc
     4801 atcgggacgg gcaggcgcac cgaattcgcc aaagaagctg gcggctacgg cgggatgatg
     4861 gtaggtccac catcagacac cacctggctg cgcatctccc accgcataga tcccaacaac
     4921 ggcaagcccg agcacgagta cagggctgcg acgagccgtg acggcgagca ctgggtgtgg
     4981 ggaggcgtat ggactttccc cgagggaacg agtacgcgcc tcgggctggt ctcgatgggt
     5041 ggatccgggg cgacggcgga attcgattac ttccgcgtat tccgcccgta accttcctca
     5101 gacacggtcc cgcgaggcgt cgtccgggca tctcagctcg aggtgcatcg tccggtagcc
     5161 cagcagacgg tgggcaagcg gaaccatgat gccgtgcgag aaaggatact tgcgggcgag
     5221 cagccgcctg gcgtgggccg actctttacc ctcaagtatc ctggcacggg cctgggtcgc
     5281 gggtcccgtc ggcctgccgc gaggcgtgca aggggcgact tcgacctccg ggttgttgcg
     5341 gattcgcctg aacttccacg ccgtgtccca ggtacggaag tacgcgtgat ccccctccac
     5401 cacgatgttg accgcggtac cgaccgggat gccgtcgcgc ttgtacgtcg tcagcaagac
     5461 tgcccactgc cggacgaatg gttcgagagc ctgcgaaaca tcaatcattg tccctcctcc
     5521 ctacggttcc tgccatgcaa gcctgaatga atgaggatcg ctacgggtag cacgacgagt
     5581 agacccagaa gtattaggat cgccaggttg tctcccacgg agaaggcgcg ggtgtgcgtg
     5641 aggtggaaga ggaagtgcgg cacggtgtag acgaggtagg cggcgagcga gacctgcacg
     5701 agacgaagat ccagcaagcc cgcggagaga gcgaggagca tgccgaaggc gaggttcatg
     5761 gcgccaacgt cccgcaccag atgctcatta tacgggccga gtgccgagac ccatcccctg
     5821 ccaggtagcg ggaagtcctc atagaaagat gcaggcaaga acagcgccca gacaccgacg
     5881 gataacgccc caacagaaat aagagcgagg ccgcctctga tccagcctgc cgacgccggg
     5941 cgtctgacgt ctacggtctc gttcatcgcg tcactccttc cgcgccgtgc aggtctatct
     6001 gcgtctctat ttgagagcgc agaaagtcct cccaccggat cttcccgtag gcacgatccg
     6061 ggcacaccag cgcgccttcg cggaacgcgc gcgccgtctt tccagggaag ggaacctcga
     6121 agatcctgcg ccgccttccc ttaatctgca ggtaggtgcg ggcggcgtcg gcaagcgtca
     6181 tgacctcggg accaccgata tcagggacgc ggcccgcggg cccggagagc gctaccaggt
     6241 ggcccgcgac ctcgccggcg tctatgggct ggccgaggaa acctcttggg atcggcatta
     6301 cgggcaacct gtctaggaac cgcatggcca tgaaaaccag gtcgtagaat tgcgtcgccc
     6361 gcaggatggt gtgcgggacc ggagagcttt cgactacacg ctcggtctcg agctttatcc
     6421 ggtagtaggg atatgacggg gcccggtcta taccgacgat ggagatgtac acgatatgtg
     6481 agacaccggc gcgggtagct gccgcgagca ggcgtcctgt cccctctacg tctacctgac
     6541 gagccttgcg gaaagggctc gttgcacagt ggatgatgat attgacatcc ttgacagcca
     6601 cttccagtcc ctcacccgtg cgaagatcac ccctgatcgt gcccggtttt ccactccggc
     6661 tgagtacccg cgactggaga cccgcggccc cgagacgctc caccacgtgc tggccgagca
     6721 cccccgtccc tcccgtaacg aggactcgcg gcgcgttcat cccgaaccct ccccgctttc
     6781 cgcctgctcc agcggcggca cgttcgctag cttctcgggg ttgacgagga gacggatggc
     6841 cgtgatgctc ccctcagcca cttcgatggt cacgacgctc tgggggctgc cgtctccgaa
     6901 gtagccgacg aagcccggcc tagcgttgac ccgcgtttgc cttacggcga agtctggagg
     6961 ggccgtccta agtatccctg agaggaaccg ggcgaccttg tccgaaccgt agatcggctt
     7021 cagcgcggcc cgggtcttcc ctccgccgtc ggagtagagg accacgtcct ccgacagcag
     7081 cgcgagcaga gcctccatgt cgccgctgag gctggccctg aggaagctgt ccatcaggcg
     7141 ttcctcctgc tccggggaac tctcgaagcg gggccttcgc gcggccacag actgtcttgc
     7201 gcggcgcgcg acctggcggc agttggcctc gctcttgccg acaagagtag ctatttccga
     7261 gtagtcgtag gcgaagacct ccctgagcag gaacaccgcc cgctcgaccg gggtgaggct
     7321 ctccagcagt acgaggaacg ccatcgaaag cgagtcgtcg aggtctgcct tgcctgcgat
     7381 ctcctgttct tcaagcagag gctcggggag ccaggggccg acgtactgct cgcggcgcgc
     7441 cctcgcggaa cgcagccggt cgatgcaaag gcgggtgacg accgtcgaga ggtaggctga
     7501 aggagtgcgc acctcgtctt cagaagcccc ttcccagcgc aggaaagctt cctggaccac
     7561 gtcctcggcc tccatgacgc tcccgagcat cctgtaggcg atcgagaaca acagcggcct
     7621 gttcctgtcg aagacctcga ttcggctcat cgcggccaaa gctcctcact atttcgctct
     7681 acaaataaga cgaaacagcc aggtccattg tgacaaaacg caaggtgaag attaacgctg
     7741 agggatcaga cgcccccaaa aaccacgtct agcgccgccg cgtaggcgtt cagcggcctg
     7801 ttgtcgtcca tgggcacgac cgtcacgtcc gcgccccgcc ccacgacaac ggcgagggcc
     7861 gggtcgttgg tgtccatgac cgcgaccgtc gggacaccgg aggcgatcgc cgccccagcg
     7921 aagccgtgat ccgcgaccac aagatctacg tccccagttt gccgcagaag gtcccgcatg
     7981 ggtcctgtgt cgtgggtgtg gaaaaggctt gccccgtctc cgagggctgc caccgactcc
     8041 gcccaatcca gcgacacgcc tcgctggtag cgcccgcgcg tctcaacagt aaccacctcg
     8101 ccgcctaatt cctccgccca cctggcgagc ccgagatagt acaggatcag ggctcccgga
     8161 tgcccagtcg cgaagatgag gcgtccgcct gagcccgcga cctccctgat acgctctccg
     8221 gcctccacga gacctgaggc cgtgcgcgac gggtctatgc acccccgccc ctcggtctcc
     8281 tctttgtcag gggggtttcc gatctgaaag gcaacggcat tgtatgcctc ttcgaagctc
     8341 acgtcctgca gcaattcctc catgccgaac gagttggccg cgtccttctc cagcaacaac
     8401 cctatcttgt agaggttgtt ctcggcaccg tgggaggtgc aggcaccggc aacgcctgcg
     8461 tcgaggagcc ggtccgcata ctgtgcgaca aggggattca cagagcaaga gtttagcaga
     8521 gtacgactat tgcaagagga ccgtcgacgc caggatctaa accgcggcgc ggctctcttc
     8581 gacctcttcg agggggatcc aatcctgcga ccagcgcgag agggcctcga ccacctcgtt
     8641 cagagaacgt cctttctctg tgagacgata ctccacgcgc acaggccgct caggcacgac
     8701 tatacgctct actatcccct cagcctcgaa ctccctcagc cgctccgaga gcatcctgtt
     8761 cgaaaggtcg gggatcacct cctgtatctc cccgaaccgc gaagcaccag tgagcatcgc
     8821 ccgcaatatc gcaccactcc acctgcgccc aacgatctca accgcccgct gatacctggg
     8881 acagaacctc tcagaatata tgtcgcccgt gctcctcatc gtgtgtggat tctaccataa
     8941 tcgcgacctt gacgtaacta agatatgtaa ttatacttgg ttacgttatg taactagtga
     9001 ccctgggagg ttgcggatgg tagatgttgg cttgcttgtt ttgagggtgg tattgggagt
     9061 gatcttcgcc gcccacggcg cccagaagtt gttcggtgcg ttcggggggc ctgggcttca
     9121 gggcacggct ggtttccacg ggcagctagg cataaggccg ccatacttga tggcggttct
     9181 cgccgggctc gcggagttcg ccggggggat tctgatcgcg gtcgggttcc tcacgccgct
     9241 cgcggctgtg gccctgatcc tgacgatggc cgtggccgtg ttcacggtgc atctgaagaa
     9301 cggtttcttc agcatgaacg gtggctacga gttcaacctg gcactcgccg ggatggccct
     9361 gacactgctg atcaccggcg ccgggactct cagcctcgac gccgtcctcg gcatagtcct
     9421 ctggtaaaag ttcttgaaag gagaaagcat gaacgtaact atcatcggtt cgggcaacat
     9481 ggcgcggggc atcggcagcc ggctcgtcgc cggagggaac agcgtccgca tcatcgaccg
     9541 caacgaggag agagctgatg cgctcgcggg cgagctgcgg gcgcaggacg gcgtcgaggt
     9601 cagcacaggg tcgttcgggg accggatcga ggacgacgtc gtcgtgctcg ccgtccccta
     9661 cgacgccgtc gagtcgatag cggatcagta ctcctccggg ctctctggca aggtagtcgt
     9721 ggacatcacc aaccctgtcg actgggcgtc cttcgacagg ctcgttacgc cgcccgacag
     9781 ctccgcggcc gaggagatcg acagcctgat ccccgaagca gcggtcgtga aggcattcaa
     9841 caccaccttc gccagcacgc tcgtcggtgg ggaggtgggc ggccagccgc tcgacgtatt
     9901 cgtcgcctcc gacgatcccg atgcgaagca gaaaatctcc gggctcatcg aggccggtgg
     9961 gctacggccc gtggacgccg gtccgctcag gagggcgcgc caactcgaag gactcgggtt
    10021 cctgcacatg acgctccagc agccgctcgg taccggcttc gggagcgcgg tcaaggtcgt
    10081 cgcctgaaga cctctcgcat aaccaaaggg cggcggcctt cgccgccctt ctacgtgtgc
    10141 cgataagttt cgatgacctc acgaaaggaa gtcgcttatg agtagtgcat ccatccccgc
    10201 acaagccagg atcgggcacg ttcacctcag ggtcgccgac ctcgaccggg cgatccggtt
    10261 ctaccgcgac ctgctgggct tcgacctttt gatgcgcttc ggcgatgagg ctgctttcct
    10321 ctctgcaggc ggctatcatc accacatcgg attgaatacc tggcagagca aggatggaga
    10381 gcctcccgcg ccgggaacta cgggcctgta ccacttcgca atcaactacc ctacgcgcgg
    10441 agatctggcc gttgccttca agcggctcct ggacaacggg tggtctattg acggggcctc
    10501 ggatcacggc acccacgagg ccatctacct gcgcgacccc gactccaacg gcatcgagct
    10561 ggcctgggac agagaccaga gccagtggcc gcggaagggt gaagagctga ccttcacgag
    10621 acggccgcta gacttcgatg gcctgctcgc cgagctggag gggcccgtcg cgcgctagcg
    10681 cgaggaagac actctatcag ggcgtgtccg gcgcgaggcg cagcaattct tgcggcatcc
    10741 tgacgggttt gcccgtggac aggtccacgc aggcgtggcg cgtgtggcct gtggtgagta
    10801 cttctccgtc gcgaagcacc tcgtattcga agcgcagaga gaccttgccc aggtcggtga
    10861 ggtctgtgcg gagggtcagc tcgtcgtcga agaaggctgg tctgcggtaa tgcagcaggg
    10921 cctcaacaac gacgaggccg cggccgcggt cttccatctc tctgtaagat tggcctgtgg
    10981 cgcgcagcca ctcgacgcgg cctacttcga agtaggagag ataggttgcg ttgttgacgt
    11041 gcccctgggc gtcgatctcg gcgtatcgga cccggatttt cgtctcgtag attatcttcc
    11101 ctcgaagttc ggcttgcgct tctcgccgaa ggcgttcatg ccctcgcgtg cgtcctccgt
    11161 ggcgaagagc agggcgaact ggtccgcctc gtactccagg ccgctaacca ggtcgacgtc
    11221 gagcgccctg ttggctgcgg ctttcgcgtg cctgacggcg acgggcccgt tggcggagat
    11281 ctcagccgcc agctccatag ctgtgtcaaa agcctctccc tggctgacga cgcggttgac
    11341 gagacctatc tcgcgggctt cttccgctcc tatacggcgg cctgtaaaga tcaactcctt
    11401 cgccagagcg ggtccgatca gacgcggcag gcgctgggta cctcccatgc caggcagtat
    11461 cccgaggccg acctcgggga aaccgaaggt cgcgttctcg gcggctatcc tgacgtcgca
    11521 cgcgatcgcc acctcgcagc cgccaccgag ggcgaagccg ttgacggcgg ctatcgtcgg
    11581 cacggggctc ctgtcgagca gggccatcgc ggcgtgaccg atctccgaga agcgcttggc
    11641 ctcgatgggg tccatgacgc tcatggccct tatgtcggcc cccgcgataa aggcccgctc
    11701 acccgcgccg gtcacgacga tcgcgcgcgg cccttcggct tcgagttcca gcagggcctg
    11761 cccgatctcc tcgaccacct gctggtccag ggcgttcaac ttctcctggc ggtcgatggt
    11821 caggacggcg acaccgtcct cgcgttccaa cttcacgtaa tccatttgcc tccctctggt
    11881 cggcagtttg tcagctagtc agcatttcag ctagtcagca cgccgccttt ggcggctttc
    11941 agcaagctgc ctggtcgcag tctgaaccct aacctcaagg gtataccctc ctccggcctt
    12001 ccctcaagca caccagcgtt ccaccacgca ctacaaatag ctgacaggct gacaagcgga
    12061 ggccgcgggc cgtagcgtgc tgaaatgctg actagctagc aggcactgcc tgctcgacgt
    12121 actctacagc tttcttcgtc ggagttccgg gggtgaagac ctcgccgacg ccgatctcct
    12181 tgaggctggc aatgtcttct ttggggatgg tcccgcccac gaacacgagg atgtcctcgg
    12241 attcgttctc cttgaggagg tctactatgc gtggcaccag cgtcatgtgg gcgcctgaga
    12301 ggatggagat gccgatggcg tccgcgtcct cctggatcgc cgcctcgacg acctgttcgg
    12361 gggtctggtg gaggccagtg tagatgacct ccatccccgc gtcgcggagg gcgcgggcga
    12421 tgatcttggc ccccctatcg tgcccgtcga ggccgacctt cgcgacaacc acccttatcg
    12481 tatgcgccat agtgacccca ttctaaccgc agtcacaatc ccgcgatacc ggccgttacc
    12541 ttctgagttc gcgcaggttg tcccagagga cgtagaggcc gaccacgagc acggcggcga
    12601 cgacgagttg ccagaaaaac tcgtagaaga tcacgagcgt ggcgaccacc gcgagcccga
    12661 tgttggcgct gctcaccagg ttgaccaggc ggcccctgaa accggcctcg gcgaaggcga
    12721 agaaggcaat gctgaccacg agcatggaga tcaggtgttc ccgctcgaac atccagatcc
    12781 cgacgaagct gatgagcatg aggctgacgc tcacggcggc ccacgcctcg gccacgcggc
    12841 ttaccctcag ctcgtcttcg gaggcgggc
//
LOCUS       DATAIR010000190         5669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_328489, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000190 DATAIR010000000
VERSION     DATAIR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328489"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VHM69_04560"
     CDS             complement(<1..404)
                     /locus_tag="VHM69_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739696.1"
                     /translation="MMETLFGTWCVCWRRAVLAVFAALLAVLAMTVPAHAGTVRSNTT
                     AITVNDNAAASVYPSTITVSGLPRKITDLNVSLNGISHTFPDDLDILLVGPGGQKVML
                     MSDAGGDPNLNNANLIFDDDPAPPLDNGGISC"
     gene            complement(583..831)
                     /locus_tag="VHM69_04565"
     CDS             complement(583..831)
                     /locus_tag="VHM69_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739697.1"
                     /translation="MTVFRYGREHFEEGEYVRRVDAMPGDDLDPVVRIVEDLGTVEIP
                     CGKVDCRSNCLEYMVRAAGRRDDYTLAECKISALVSII"
     gene            974..2467
                     /locus_tag="VHM69_04570"
     CDS             974..2467
                     /locus_tag="VHM69_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2739698.1"
                     /translation="MEKTLGRTLTRREFLKVAGAGMAGASLLAGCGRSHLLPDLPGSN
                     PSADRDTNVVLIIIDSLRKDHVGVYGNDRIRTPNLDALAKESLRFTRAYPESAPTICA
                     RRAIHTGLRTWPFTDWKRYKGIDVGLQGWQPIPNDQTTLAEMMRRAGYETVFVTDNLQ
                     QYDASMNFHRGFDAFDFIRGQTTDPYRPMWTCPPGKVNGTLVRGEGSGTGGSAYFKQY
                     FANTADRRVEDDWFAPQVFTRASESLEILSKGGGPFFLTVDCYDPHAPWDPPDEYVAM
                     YSDGYEGPEPYAPADGSIEGFTERHLGRMGALYSGEVTMMDRWLGRFLDKMEELNLFE
                     NTLLILLSDHGVAHGEHGIVGKTPSSLWPEVTDIPFFIRHPGGKGAGQTNDYYASTHD
                     VAPTILGFLGVETQQELDGQDLSVMIEGDEPEARPHFTLGYHDHVFTRDEEYAMISAN
                     DGTEAKLYNLREDPGMYKNVAVERPDVTRRMFDDYVLEDAGGPLPSY"
     gene            2506..3369
                     /locus_tag="VHM69_04575"
     CDS             2506..3369
                     /locus_tag="VHM69_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX2739699.1"
                     /translation="MSRTFYIVDVFAEEKYAGNQLAVVRGGADLPDEVLLKIAMEMNY
                     SETTFILTEKERDGGYDVRIFTPGGEVPFAGHPTLGTAYVIRQEILAEPVESVTLNLK
                     AGRVPVTFGDVLWMRQLQPTFGPTLDPAPFTGVLDLDEADLDDLYPVQEVSTGLPAII
                     VPLRSLDALRRCRFDRDRYLELGGSGKNLYAYCPESHGENTGEMSARMFANDLGFAED
                     PATGSAAGCLAGYLVEHRYFGTDSIDVRVEQGYEIDRPSLLYLRATRDGEETSVHVGG
                     KVQMVARGELV"
     gene            3366..3878
                     /locus_tag="VHM69_04580"
     CDS             3366..3878
                     /locus_tag="VHM69_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2739700.1"
                     /translation="MSRDTGKVEVRDAGVDDAREIKSVARTTWDHTYRDTIPEDVRAA
                     FVDQAYSADSLRRRIESNVFLVAAEGEEVLGFADFRPLSDTEAGLAAIYVLPAMQGRG
                     IGARLLEAGIGAFPSRTRFVLRVEQDNALAQRFYEAHGFRRTGVHTDAFYGQVVHEVE
                     MTLHAPDFAP"
     gene            4019..4846
                     /locus_tag="VHM69_04585"
     CDS             4019..4846
                     /locus_tag="VHM69_04585"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2739701.1"
                     /translation="MPDATSPVPADQPQSSTTGDLRGRVALVTGVGRKRGIGSAVCRE
                     LASRGADVVLSYWKPYDREMPWASDEDEPETLLGELRAAGVRAEGVEADLSRPDSPQL
                     LLDAAEEMIGRPSILVNVAAYSARDGFEALDAETLDAHYAVNVRAMALLSVLFARRYP
                     GGPGGRIVNFSSGQSLGPMPGELAYIMTKGAIEAFTRTLAAEVGHKGITVNAINPGAT
                     DTGWMTEEMKREIVTKSPSGRIGQPEDAARLVAFLAGDEGAWITGQIIHSEGGFFRS"
     gene            4901..5290
                     /locus_tag="VHM69_04590"
     CDS             4901..5290
                     /locus_tag="VHM69_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739702.1"
                     /translation="MPKLFRGPPKTLFDDAEDEDFEAPAKGPLFSGSVFYALLAFFGV
                     SLMLTADWFGLLLLNSEGVFGRIGPLNSVRVTAILMTGIAIPSALLMFHYKRLYSYTS
                     DENVFFAITFSLSFLLGCPCALASIFG"
     gene            complement(5333..>5669)
                     /locus_tag="VHM69_04595"
     CDS             complement(5333..>5669)
                     /locus_tag="VHM69_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2739703.1"
                     /translation="HEHVPRTGASAYCASKGGLGMLTKVMAMELAEYGILVNAVAPGE
                     ISTPMTGAEDEDPTTIERPTLPLGRPGHAREIAAWVAFLASEKSSYATGASFVVDGGL
                     MLMAAELSS"
BASE COUNT         1090 a         1773 c         1792 g         1014 t
ORIGIN      
        1 caacacgaga tcccaccatt atccagtggt ggtgctggat cgtcgtcgaa gatcaggttc
       61 gcattgttca ggttgggatc gccgccggcg tcggacatga gcatcacctt ctgacccccg
      121 ggacccacga ggaggatatc aaggtcatcc ggaaaggtgt ggcttatgcc gttgagcgac
      181 acgttcaggt cggtgatctt gcgcggcaag ccactgacgg tgatcgtcga tgggtacacc
      241 gacgccgcgg cgttgtcgtt tacagttatg gccgttgtat tggaacgtac ggtgcctgca
      301 tgtgcaggga cggtcatcgc aagcacggcc aacaaggcgg caaagacagc caacacagcc
      361 cggcgccagc agacgcacca ggtcccgaat aaggtctcca tcatccgatt gctccttcta
      421 tttactcgtg gggagaagag atcccccgca acagaaacag agtgcaggat acgccctgga
      481 ccggcgcggc gtatcttaca aatgggttat cttcttctag cccaaatggg ctagtttcgt
      541 ccgaggtcag gcaccacgca ggccggtacc agaaggctat gcctagataa tgcttacgag
      601 cgcggagatc ttgcactccg ccagggtgta atcgtcgcgc cttcctgcgg cgcggaccat
      661 gtactccagg cagttgctgc ggcaatccac cttgccgcag gggatctcga ccgtccccag
      721 atcctctacg atgcgcacca cggggtcgag gtcgtcgccc ggcatcgcgt ccacgcggcg
      781 cacgtattcg ccctcctcga agtgttcccg cccgtagcga aaaacagtca taaataaatt
      841 gtacaccggt ttgacctgga tcacatcgag agcctggatg ccatgctcgc ggcgattgcg
      901 ggtacgatga gatttcccgc ctcggcttgc tatatgattg gcggggcctc taaagaggta
      961 tcgagtaaac aggatggaga agaccctggg acggacgtta acgcgaagag agttcttgaa
     1021 ggtggcgggt gccgggatgg ccggggcatc gctgctcgca gggtgcggac gcagtcatct
     1081 actgccggac ctcccgggca gcaacccgtc cgcggacagg gacaccaacg ttgtccttat
     1141 catcatcgac agcttgagga aggaccacgt cggcgtctac gggaacgatc gcatccggac
     1201 gccgaacctg gacgcccttg cgaaagagag cctgcgcttt acccgcgcct accctgagtc
     1261 ggcgccgacg atctgtgcga ggagggccat acacacgggc ctgaggacct ggcctttcac
     1321 tgactggaaa cgttacaagg gcatagacgt ggggctccag ggttggcagc cgatccccaa
     1381 cgaccagacg acgctcgcgg agatgatgcg aagagcgggt tacgagacgg tgttcgtcac
     1441 tgacaacctg cagcagtacg atgcctcgat gaacttccat cgcggctttg acgcattcga
     1501 ctttatcagg ggacagacca ccgaccccta ccgccccatg tggacatgtc cccctgggaa
     1561 ggtgaatggg acacttgtga ggggcgaagg gagcgggacg ggaggcagtg cttacttcaa
     1621 gcagtatttc gccaacaccg cggaccgcag ggtggaagac gattggttcg cgccccaggt
     1681 cttcacgcgg gcctcggagt ccctggagat cttgagcaag ggcggcggac ccttctttct
     1741 cacggtggac tgctacgatc cccacgcccc atgggacccg ccagacgagt acgtcgcgat
     1801 gtacagcgac ggttacgaag gccccgagcc ctatgcgccg gccgacggca gtatcgaagg
     1861 ctttacggag aggcacctcg ggcggatggg cgccctctac tccggcgagg taacgatgat
     1921 ggaccgctgg ctcgggcgct tcctcgacaa gatggaggag ttgaacctct tcgagaacac
     1981 cctcctcatt ctcctctccg accacggcgt cgcccacggc gagcacggca tcgtcggcaa
     2041 gaccccctcc tctctgtggc ccgaggttac ggacatcccg tttttcatcc gccatccagg
     2101 gggcaaagga gccgggcaga cgaacgacta ctacgcctcg acccacgacg tggcgccgac
     2161 gatcctcggc ttcctcggcg tagagacaca gcaggagctg gacggccagg acctctccgt
     2221 gatgatcgag ggcgacgagc ccgaggcgag gccgcacttc acgctcggat accacgacca
     2281 cgtcttcacc agggacgagg aatacgccat gataagcgcc aacgacggca cggaggccaa
     2341 actctacaac ctgagagagg accccggcat gtacaagaac gtcgcagtag agcgccccga
     2401 cgtaacgcgg aggatgttcg acgactacgt tctggaggat gccggaggac ccctgcccag
     2461 ctactgaccc tcagcagaca gtccgactac ggtgaggaga gatctttgag ccggaccttc
     2521 tacatcgtgg acgtgttcgc ggaggagaag tatgcgggca atcagctcgc cgtggtccgc
     2581 ggcggcgcgg acctgcccga cgaggtcctc ctgaagatag ccatggagat gaactactcc
     2641 gagaccacgt tcattctcac ggagaaggag cgcgacggcg gctacgatgt ccgcatcttc
     2701 acgccgggcg gggaggtgcc tttcgccggc cacccgacgc tcggcacggc gtatgtgatc
     2761 cggcaggaga tcctggccga gccggtcgag agtgtcacgc ttaacctgaa ggcgggcaga
     2821 gttcccgtga cttttggcga tgtgctctgg atgcgccagc tccagccgac cttcggcccg
     2881 acccttgacc ctgccccctt cactggcgtt ctcgacctcg atgaagcgga tctggacgac
     2941 ctctatcctg tacaggaagt atccacgggc ctgcccgcca tcatcgttcc actgaggagc
     3001 ctagacgccc tgcgacggtg caggtttgac agggatcgtt acctcgaact ggggggttct
     3061 gggaagaacc tgtacgcgta ctgtccagaa tcccacggcg aaaacaccgg cgagatgtca
     3121 gccaggatgt tcgcaaacga cctcggcttc gcggaggacc ccgcaacagg aagcgccgcg
     3181 ggctgcctcg ccggatacct cgtcgaacat cgctacttcg ggaccgactc catagacgtg
     3241 cgcgtcgagc aggggtatga gatcgaccgc ccctccctcc tgtacctgcg ggccacaagg
     3301 gacggagaag agacgagcgt ccacgtcggg ggcaaggtgc agatggtggc gagaggcgag
     3361 ctggtgtgag ccgggacacc gggaaagtgg aggtccgtga cgctggcgtc gatgacgcac
     3421 gggagatcaa gagtgtggcg cggacgacct gggatcatac ctaccgggat actatccccg
     3481 aagacgtgcg ggcggcgttc gtcgaccagg cctactcggc ggactcgctc aggcgcagga
     3541 tagagtcgaa cgtcttcctc gtcgcggcgg agggtgagga agtcctcggt ttcgcggatt
     3601 tccggcccct ctccgacacg gaagcggggc tcgcggccat ctacgtcctt cctgcgatgc
     3661 agggacgggg gatcggggcg cgcctcctcg aggccggtat cggcgccttc ccgtcgcgta
     3721 cgaggttcgt gttgcgggtg gagcaggaca acgcgctggc ccaacgcttc tacgaggccc
     3781 acgggttcag gcgcaccggg gtgcatacgg acgcgttcta cgggcaagtg gtgcacgagg
     3841 tggagatgac cctgcacgcc ccagatttcg ccccttgaac gggctcgact ttggagcagc
     3901 gtcaggcggc ttgagacgcg ctcctcgccg tgctagcttg atacgctggc tataggttta
     3961 ctgaccctca gccgagttct tcatgcccgc caggcaggag ctacaggagg agaagcccat
     4021 gcccgacgcc acatccccgg tcccagccga tcaacctcaa tcctccacca caggtgacct
     4081 gcgtggccgc gtcgcgctcg tgaccggcgt agggaggaaa agggggatag ggtccgcggt
     4141 ttgccgggag ctcgcctccc gcggcgccga cgtcgtccta tcatactgga agccatacga
     4201 ccgcgagatg ccctgggcgt cggacgagga cgaaccggag acgctgctcg gggagttgcg
     4261 ggccgccggc gtcagggcag agggggtcga ggccgacctc tccaggcccg attctccgca
     4321 gttactcctc gacgcggccg aggagatgat cgggaggccc tccatcctcg tcaacgtcgc
     4381 ggcctactct gctcgggacg gcttcgaggc actcgacgcc gagacactgg acgcccacta
     4441 cgccgtcaac gtgcgcgcaa tggcgctcct cagcgttcta tttgccaggc gttacccggg
     4501 cgggcctgga ggcaggatcg tcaacttcag ctcggggcag tcgctcggtc cgatgcctgg
     4561 ggagttggca tacatcatga ccaaaggcgc catcgaggcg ttcacaagga cgctcgccgc
     4621 cgaagtcgga cacaagggta tcaccgtaaa cgccatcaac cctggtgcca ccgacacggg
     4681 ctggatgacc gaggagatga agcgtgaaat cgtgacgaag tccccgtcgg gcaggatcgg
     4741 gcagcccgag gacgcggcga ggctcgtggc tttcctggcc ggtgacgagg gggcctggat
     4801 caccggccag atcatccact ccgagggagg tttcttccgt tcttgagcgg cacggcctcc
     4861 ttgcgttacc atgacgtcct tcatggagac ccccagaaaa atgccgaagc tcttccgtgg
     4921 accgccgaag acgctgttcg acgacgctga ggacgaggac ttcgaggcgc cggcgaaggg
     4981 tccgctgttt tcgggctccg tgttctacgc gctacttgcc ttcttcggtg tctccctcat
     5041 gcttaccgcc gactggttcg ggctcctgct cctcaactca gaaggcgtct tcgggcggat
     5101 cgggcctctc aattccgtgc gcgtgacggc catcctgatg acggggatcg ccatcccctc
     5161 agccctcctg atgttccact acaagcgcct ctactcctac acctcggacg agaacgtctt
     5221 cttcgccatc accttctcgc tctctttcct cctcggctgt ccctgcgccc tcgcgagtat
     5281 attcggttag ctgatgacga ggtttgtggt actgaacttc cgcaagaccg ctttagctcg
     5341 acaattccgc ggccatgagc atcaggccgc cgtccacgac gaatgaggcg ccggttgcgt
     5401 aggatgactt ctccgaggcg aggaaggcca cccaggcggc gatctcacga gcgtgtccgg
     5461 gacgtccgag cggcagggtc gggcgctcga tagtcgtcgg gtcctcgtcc tcggcgccgg
     5521 tcatgggagt agagatctca cctggagcca ccgcgttgac caggatgccg tactccgcca
     5581 gctccatcgc catgaccttg gtcagcatcc caaggccacc tttcgacgcg cagtacgcgg
     5641 aggcacccgt gcgcgggacg tgctcgtgg
//
LOCUS       DATAIR010000191         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_51594, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000191 DATAIR010000000
VERSION     DATAIR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51594"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHM69_04600"
     CDS             <1..588
                     /locus_tag="VHM69_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2739704.1"
                     /translation="PDRIEGQKTAAFEVAEVLGHPPDALALPVGNAGNITSYWKGFRE
                     LRESGRSDSVPRMLGFQAEGASPLVAGHDFENPETVASAIRIGSPASKEGALSAARDS
                     GGGIESVTDAEILAAQEMLAHEEGVFCEPASAAGIAGLLKLVGEGRGPEGTVVSVLTG
                     HGLKDPDVILGRVQLPDPVPATFEAVAGQMGRAIA"
     gene            594..1511
                     /gene="thrB"
                     /locus_tag="VHM69_04605"
     CDS             594..1511
                     /gene="thrB"
                     /locus_tag="VHM69_04605"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2739705.1"
                     /translation="MRVPATSANLGPGYDAVGLALSLSMRIGLDRAPQPAIEVHGTGA
                     DLIPTGPEHPAYRAARFVAELVGESDIHFSLVQENHIPPTRGLGGSAAALVGGAVAAN
                     DLFGGQIAAPDLLNIVCELDGHPDNAAPALLGGLVIGTLTPTGVSAVRLEPKNLKVVV
                     AVPDFAVSTTAARQALPDSVPHRDATFNVGRSGLLLGALATGEYHLLRIAMQDRLHQP
                     YRSHLIPGLEDVIEAALASGAYGACLSGSGPTVLAFAPETNAPEIASDMRAAFEARGV
                     RAESWALDVDLAGARVEPPEVVPTPSPVF"
     gene            complement(1540..>1898)
                     /locus_tag="VHM69_04610"
     CDS             complement(1540..>1898)
                     /locus_tag="VHM69_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX2739706.1"
                     /translation="LLLAEGVLGVLAGLVALFWPGISALVLLYVIAAWAIFTGILKVV
                     MAISLRREIENEWLMILSGVLSVVFGVVLGALPGVGLLSLVWLVGIYALIFGVALIVL
                     GFRVRGHRRENSGEMS"
BASE COUNT          339 a          696 c          588 g          275 t
ORIGIN      
        1 cccgaccgca tcgagggcca gaagaccgcg gccttcgagg tcgctgaagt cctcggacat
       61 ccccccgacg ccctggccct gccggtgggg aacgccggca acatcacctc gtactggaaa
      121 gggttcaggg agttgagaga atcgggacgc agcgactcag tcccccgtat gctcggcttc
      181 caggccgagg gtgcctcacc tctcgtcgca ggccacgact tcgagaaccc cgaaaccgtg
      241 gcgagcgcca tccggatagg ttcgccggcg agtaaagaag gggccctctc agccgcgcgc
      301 gattccggcg gcggcatcga gagcgtgacg gacgccgaaa tcctcgctgc ccaggagatg
      361 ctcgcccacg aggagggcgt cttctgcgag cccgcatcgg cggcggggat cgccgggctc
      421 ttgaaactcg tgggcgaggg cagagggccc gagggtacgg tcgtaagtgt actcaccgga
      481 cacgggctca aggaccctga cgtgatcctc ggccgtgtgc agctgccgga tcccgtcccc
      541 gcgaccttcg aggcggtcgc cggacagatg gggagggcta tcgcttgatc tccgtgcgcg
      601 tcccagccac ctcggcgaac ctgggaccag gctacgacgc ggttgggctc gcgctctctt
      661 tgagcatgcg gatcgggctg gacagggcac ctcagccagc gatcgaggta cacggtaccg
      721 gggccgacct catccctacc gggccggagc atccggcata cagggcggcg cggttcgttg
      781 ccgaactcgt cggcgaatcg gatatccact tcagcctcgt gcaggagaac cacattccgc
      841 cgacgcgggg cctgggcggg agcgcagccg cgctcgtcgg cggtgccgtc gcggcgaacg
      901 atctgttcgg ggggcagatc gccgcccccg acctcctcaa catcgtctgc gagctcgacg
      961 gacaccctga taacgcggcc cccgcactcc tcggcgggct ggtaatcggg acgctcacgc
     1021 cgacgggcgt gagcgccgtg cgcctggaac cgaagaacct caaggtcgtc gtcgccgtcc
     1081 ccgacttcgc ggtctcgacc accgcggccc gccaggccct ccccgactcc gtcccccacc
     1141 gcgacgccac ttttaacgta ggacgctctg gcctcctgct aggggctctg gcgaccggcg
     1201 agtaccacct tctgcgtatc gccatgcagg acaggctcca ccaaccctac cgctcccacc
     1261 tcatccccgg cctcgaagac gtcatagaag ccgcgctagc cagcggcgcc tacggcgcct
     1321 gcctctccgg ctcgggcccc accgtcctcg ccttcgcgcc cgagacaaat gcccccgaga
     1381 tagcgtcaga tatgcgggcg gccttcgagg ctaggggcgt gcgcgccgag tcgtgggcgc
     1441 tcgacgtcga cctcgcaggg gcccgcgtcg agccgccgga agtcgtcccg acaccgagcc
     1501 ccgtgttcta gggtctactg ctcggataat tgagccgggc tagctcatct ctccgctgtt
     1561 ctccctgcga tggccccgca ccctgaaacc caggacgatc agcgccaccc cgaagatcag
     1621 cgcgtagatg ccgaccagcc acaccaacga gagcagcccc acgccaggca atgcacccag
     1681 gaccaccccg aagactacag agagcacacc gctcaagatc atcagccact cgttctctat
     1741 ctcccgcctg agcgagatgg ccatgaccac cttcagtatg cccgtgaaga tcgcccacgc
     1801 cgcgatcaca tacagcaaca ccagggccga tatgccgggc cagaacagag caacgaggcc
     1861 cgccagcact ccgagcacgc cctcggccag aagcagcc
//
LOCUS       DATAIR010000192         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_236428, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000192 DATAIR010000000
VERSION     DATAIR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236428"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1183)
                     /locus_tag="VHM69_04615"
     CDS             complement(215..1183)
                     /locus_tag="VHM69_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2739707.1"
                     /translation="MQPVGEVAEVVDGVYQLKLPVPFPLRFVSVYLIKGGDGWTLVDT
                     GYDYPPNYEVWEAGAVEVGCDLALDVARIVVTHFHPDHLGGARWLQEKSGAPVFMLEE
                     EISFSRELWTAPDASPFQDHLVRNGMPREMAREGAVAMRSSLPLPDEMRPLRPGEKLS
                     LGDGAARVIHTPGHADHQFVLHDEARGILFVADQVLLGITPNIGLWLESEPHPLCRYL
                     ESLDELRGLDVALVLPGHGPVFHDLQGRIGEISTHHYGRLEAMHAVIEAGPMTPYAVS
                     RLVFRGALTVHQRCFALAETLAHLDHLALEGRAKRIENARVAYLAM"
     gene            complement(1280..2458)
                     /locus_tag="VHM69_04620"
     CDS             complement(1280..2458)
                     /locus_tag="VHM69_04620"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2739708.1"
                     /translation="MREAVIVSGARTAVGRAPRGTLRASRPDDMAAAAIREAVERAEG
                     LAGEHVEDVIIGCAMPEGTQGHNVARLSSLLAGMPDTVPAQTVNRFCSSGLQTIAMAS
                     ERIMVGNATTIVAGGTEHMSSTIEMPNFSPNPRLVEVNPAAYMGMGLTAEQVASEFDV
                     SREDQDEFALRSHTLAKKAVESGVFDEEIIPIDVEFDWVDESGESKHMETTFARDEGP
                     RDTSAEKLANLKPAFQNGGTVTAGNSSQRSDGAAAVVVMDRAEAERRGLTPLARFVGF
                     AVGGVRPEVMGIGPTVAIPKVLKQTGLTMEDIDLIEFNEAFAAQALAVIREVGMDLEK
                     TNVNGGAIALGHPMGATGTKLTIQLLHEMKRRGSKYGLVTMCIGGGMGAAGIFENLQN
                     "
     gene            complement(2533..>3323)
                     /locus_tag="VHM69_04625"
     CDS             complement(2533..>3323)
                     /locus_tag="VHM69_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2739709.1"
                     /translation="SVGANLGEIAHAVQNGDLDQVGKSVEALQNLVMAFRFAPKPVVA
                     APRGQTLGGGLEVCLHSDRIVAAGETYTGLVEVGVGLIPAGGGTKEMARRLISPALHT
                     APNSPPLPFLQRAFEQIALAKTAASALDAREMGFLTEDDRVVMNPDHLISAAKREAID
                     LADGYAPPEHGKNVYAAGRSARAALEVGVKTMQWARYASDYDGVIAGHVARVLAGGSL
                     SLPQWVPEEYILELEKEAFLDLLKQEKTQERIEALLKTGKPKRN"
BASE COUNT          573 a         1097 c         1009 g          644 t
ORIGIN      
        1 ggatcggcgc tccttcatca ggtgctgaag gacatcgttt cgtcgtcagc caccgctccg
       61 aggaatcgct ggggcagagc ctcggcaaga ccatcctctg gttgggcgtt gggggcgccg
      121 gggcgctcgt cctcggtgtg atcctgctgg tcatcggtat catcgttctg ctaggctgag
      181 atactcgcgc atttggctat cctgaagtga gtcgttacat agccaggtag gcgacgcgag
      241 cgttctcaat ccgctttgcc cggccttcga gggcgagatg gtccaggtgc gccagggtct
      301 cggccagggc gaagcatcgc tggtggaccg tgagagcacc ccggaagact aaacgcgaga
      361 cggcataagg cgtcatgggt cctgcctcga tgacggcatg catggcttcg aggcgcccgt
      421 aatggtgggt ggagatctcc cctatccgtc cttgcagatc gtggaagaca ggaccatgtc
      481 cggggaggac gagtgccacg tcgagaccgc gcaattcgtc cagggattcg aggtacctgc
      541 ataacggatg cggttcggat tcgagccaga gcccgatatt cggtgtgatc ccgagcagta
      601 cctgatccgc gacgaagaga atgcccctcg cctcgtcgtg gagcacgaac tggtggtcgg
      661 cgtgaccggg agtatggatg acacgggccg ccccatcccc caacgagagt ttctcaccgg
      721 ggcggagcgg gcgcatctcg tctggcagcg gaaggctcga cctcatcgcg accgcgcctt
      781 cacgggccat ctcgcgcggc atgccattac gtacgagatg atcctgaaac ggagaagcat
      841 ccggcgcggt ccaaagttcc ctggaaaagg agatctcctc ttcgagcata aacaccggcg
      901 ccccagactt ttcctgaagc caccgcgctc ccccgagatg gtcgggatgg aagtgggtga
      961 caacgatgcg ggccacgtcc agcgccagat cacagcccac ctccacggcg ccggcctccc
     1021 agacctcgta attgggcggg tagtcgtagc cggtgtccac gagcgtccaa ccgtcgccgc
     1081 ctttgatcag gtagaccgac acgaagcgca aagggaaggg taccggcaac tttagctggt
     1141 aaacgccatc gactacttca gccacctcac cgacaggctg cataccaacc ttggatggct
     1201 gaactccatc gatcacagga tttcgttctt cataggacca gcggccaacc ccgcaaagct
     1261 gaccgctgat gcctgaaacc taattttgca ggttctcgaa gataccggcc gcccccatcc
     1321 cgccaccgat gcacatggtg accaggccgt acttcgagcc gcgccgcttc atctcgtgga
     1381 gaagctgtat cgtgagcttc gtacccgtcg cgcccatcgg gtgtccgagc gcgatcgcac
     1441 caccgttcac gttggtcttc tcaaggtcca tgccgacctc acggatcacg gcgagagcct
     1501 gcgccgcgaa cgcctcgttg aactctatga ggtcaatgtc ctccatcgtg agccctgtct
     1561 gcttcaggac cttcgggatc gccaccgtcg gaccgatccc catgacctcg ggccgcaccc
     1621 cgcctaccgc gaacccgacg aagcgggcga gcggcgtgag ccctcgcctc tcggcctctg
     1681 ccctgtccat aacaacgacc gcagcagccc cgtcactgcg ctgcgaagag ttgcctgccg
     1741 tgacggtgcc cccgttctgg aaggcgggct tgaggttggc gagcttctcg gccgaggtgt
     1801 cccgcggccc ctcgtcgcgc gcgaacgtcg tctccatgtg cttcgattcg ccgctctcgt
     1861 ccacccaatc gaactccacg tcgatgggta tgatctcctc gtcgaacaca ccgctctcga
     1921 cggctttctt cgccagagta tggctgcgga gcgcgaactc gtcctggtcc tcgcggctca
     1981 cgtcgaactc gctggcgacc tgctccgccg tcaatcccat ccccatgtag gccgcagggt
     2041 tgacctccac gagccgcggg ttcggcgaga agttgggcat ctcgatggtc gaggacatgt
     2101 gctcggtccc acccgccacg atggtcgtcg cgttgccgac cataatgcgc tcggaggcca
     2161 tcgcaatcgt ctggagcccc gacgaacaga acctgttgac cgtctgcgcg ggcaccgtgt
     2221 caggcatccc cgcgagcagc gatgagagcc tggcgacatt gtgcccctgc gtcccctcgg
     2281 gcatcgcaca cccgatgatg acgtcctcga cgtgttctcc cgccagccct tccgcccgct
     2341 cgacagcctc ccggatcgcc gcggcggcca tgtcgtcagg tcggctggcc ctcaatgtcc
     2401 ccctcggagc tcgtccaacg gcagtccgcg cccccgaaac aattaccgct tctcgcattt
     2461 ttctctccct tcggtcgctc aggtttctct aacgcaagtc ctacctgaca cctgaagcct
     2521 gatgcctaat agctaattcc tcttcggctt tcctgtcttt agtaaggctt cgatgcgctc
     2581 ttgggtcttt tcttgtttta ggagatccag gaacgcttct ttttccagct cgaggatgta
     2641 ctcctcaggc acccactgcg ggagcgagag gctgccgccg gcaaggacgc gggcgacgtg
     2701 gcccgcgata acgccgtcgt agtcgctcgc gtagcgagcc cactgcatgg tttttacgcc
     2761 aacctccagc gcggctcggg cggagcggcc cgcggcgtag acgttcttgc catgctccgg
     2821 cggcgcgtag ccgtcggcca ggtcgatggc ctcgcgtttg gctgcggaga tgaggtggtc
     2881 tgggttcata accacgcggt cgtcttcggt gaggaagccc atctccctgg catcgagggc
     2941 gctcgcagct gtcttggcga gcgctatctg ctcgaaggct ctctggagga aaggcagtgg
     3001 cggggagttt ggcgcggtat gaagcgccgg ggagatgaga cggcgcgcca tctcctttgt
     3061 gcccccgccg gcggggatca ggccgacccc aacctcgacg agacccgtat acgtctcgcc
     3121 cgcggcgact atgcgatcgg agtgcaggca gacttcgagt ccgccaccga gagtctgtcc
     3181 gcgcggcgcc gcgacgacgg gcttcggggc gaagcggaag gccatcacta ggttctgcag
     3241 ggcttcgacg ctcttgccga cctgatccag gtccccgttc tggacggcgt gggcgatctc
     3301 gccgaggttc gcacctactg aga
//
LOCUS       DATAIR010000193        22965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_12298, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000193 DATAIR010000000
VERSION     DATAIR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22965
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12298"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1154)
                     /locus_tag="VHM69_04630"
     CDS             complement(159..1154)
                     /locus_tag="VHM69_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX2739710.1"
                     /translation="MRPRGLYVLASDAFDRIYGEPERADIAALVEVEDVPQTAETVAK
                     NLDVLDGVEVILSGWGAPVFDGAFLEAAGDLRAVFYGAGSVRSFVTDALWKRNVRVVS
                     AYSANAVPVSEYALAAILFSLKRGWRLAASVKQERSFPTVSAMPGAYGSTVGIISLGA
                     TGRMLRERLRPFDVRVVAHDPYVTIEEAAELGVELMTLDPLFMEADVVSLHTPWLPET
                     EGMIRGSHLASMKQGATLVNTSRGAVVREGEMVSVLKDRPDLTAVLDVTHPEPPEPDS
                     PLYDLSNVVLTPHIAGSQGDECRRMGRLVVDELRRYVAGEPLEHEITKERAAVMA"
     gene            complement(1151..1984)
                     /locus_tag="VHM69_04635"
     CDS             complement(1151..1984)
                     /locus_tag="VHM69_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2739711.1"
                     /translation="MSGDLSRLSLNQATTRQWGVREAAEGCARAGIPWIGLWRDKVSE
                     TGLEKSARIVREMGLKVSSLCRGGMFPAATERERLARLDDNRRAVDEAAALGAEVLVL
                     VCGGAPDRDIGAARGNVLDGVEAIAPYASDAGVILGVEPLHPAFAADRSVVCTLGEAT
                     GIVRRVGSPGVGVVVDAYHVWWDPALYDEIRRAAGAIVGFHVDDWPKGGGDPLLSRAL
                     MGDGVIELRRMRAAIDAADYEGPIEVEIFNEAVWATPGEEVLETVKRRYLEHVLAGVH
                     A"
     gene            complement(1981..3150)
                     /locus_tag="VHM69_04640"
     CDS             complement(1981..3150)
                     /locus_tag="VHM69_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2739712.1"
                     /translation="MLKELILPRSDGSLMQYVPQGEPLPVVSVAPPWTRVAYAAVHVV
                     ADPLADNDPTLDVALDWEATLAYRRYLWSLGFSVAEAMDTAQRGMGLDWGTAEELIHR
                     SIAEARAEGAGDRIACGAGTDHLGMSPSVTLRDVSSAYEEQCASVERAGGRVILMASR
                     ALAAAARGPEDYASVYGEVLSRLSEPAILHWLGEDFDPALAGYWGHDDLDLAMESCLA
                     IIAEHASKVDGIKVSLLDAEREIEMRRSLPEGVRMYTGDDFNYPELIRGDDEGFSHAF
                     LGIFDAIAPAASAALHALDAGDTDRYEELLAPTVPFSRHVFKAPTRFYKTGVVFLAYL
                     NGHQDHFRMVGGQEGARSVVHLSQLFVLADKAGLLADPERAAGRMRPVLDLAGVS"
     gene            complement(3143..4315)
                     /locus_tag="VHM69_04645"
     CDS             complement(3143..4315)
                     /locus_tag="VHM69_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2739713.1"
                     /translation="MRTVTVAMNGVTGRMGTRQHLVRSLVAIREQGGVTLPGGDVVVP
                     EPILVGRDETKLRRLAEAHGIERWTTDLDKCLSDPEVEIYFDSQTTTRRTESVKAAIG
                     AGKHVYCEKPTATTVEEAIEIARLAGEGRVKNGVVQDKLFLPGMMKLKRLVDSGFFGR
                     ILSVRGEFGYWVFPGDGRPSQRPSWNYREEDGGGIILDMLCHWRYVLDNLFGEVRSVS
                     CLGATHVPKRWDENGEPYVATAHDAAYATFELDGGIVANINSSWCVRVNRDELFELQV
                     DGTDGSAVAGLRECKAQHGANTPKAVWNPDLPDTNGYRSQWLDVPQVEDPDNAFKVQW
                     EMFLRHVVADEPFPYDLWEGARGVQLAELGLRSSRERRWLDVPALDAGRTRELDHA"
     gene            complement(4323..5234)
                     /locus_tag="VHM69_04650"
     CDS             complement(4323..5234)
                     /locus_tag="VHM69_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEX2739714.1"
                     /translation="MTLRLMHALGESVEARDGRDLLFRYVYEARVDASESPKPYFHPL
                     RTLAGEEVTLFRPHDHPWHTGLAMTSAYLSGENFWGGPTYVRGKGYQWLENQGMIRHD
                     DWCEIRGEGSFLKERLSWISHGGETWIEEERGISVGGIYPSEGFWSLDLSFRLKNVTE
                     RSLTFGSPTTEGRPAAGYGGLFWRGPRSFDDGEILAADGLEGPETMGRTSPWLAYIGL
                     HDGTGRASTVLFLDSPTNVRFPGKWFVRNDPYACASCSFMFDEEYVLEPEEELALDYR
                     VVLGDGRWSRERIEEYVDSIHSRVGKE"
     gene            complement(5231..6373)
                     /locus_tag="VHM69_04655"
     CDS             complement(5231..6373)
                     /locus_tag="VHM69_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX2739715.1"
                     /translation="MRKIENIEVFPVLLPVIETFQFASGSAGKAGGKAPHVFVKVTDS
                     EGEVGWGEGRPVPQWSYETLETVSTTIRNYLAPPLLGLPVADRWGLHRRMHAAIGRGP
                     STGQPVAKAAVDLAVHDLCARAAGMSLRSFLGGADERASVELSYTVTGRDAATVRDDV
                     DAARQDGFRHFNFKAGSGADLEVASCVREAAGPEAFVWADANQGYGLHEARTAAPRLA
                     GAGVDVLEQPLPADHIHLMRELRRSVQIPLAVDESIVSPADFFAHAAEGLVDYLVVKV
                     TRSGGVWPTLAQISVAEAAGLPLLVSGLTDGLLTKVAVCQVAAAFGCAGPAALNGSQF
                     TDESAIFPDKAKVESGGTVHLPADAGIGVRPDEDALRDFLAKEGER"
     gene            complement(6370..7446)
                     /locus_tag="VHM69_04660"
     CDS             complement(6370..7446)
                     /locus_tag="VHM69_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2739716.1"
                     /translation="MRKIRAAIIGTGNIARAHVAALGKVGKRVELVAAVDVDSALAAA
                     FCSEHGIPRSYVDPQQMLEEEAPDLVHVCTPPQSHRELSIQCMEAGAWVLCEKPLCAS
                     LEEMDEIEAAERATGKYCSSVYQWRFGAGARHLKALISSGELGRALVGLCQTTWYRDA
                     AYYQVPWRGKWETELGGATMGQGIHAIDLFLWMLGDWHEVRAMAGTLDRDIEVEDVSM
                     AVVRFENGAMGSVVNSVLSPRQRTHLRFDFQEATVEVDSLYGYSNSDWRYSIPDGAVH
                     ADSLARWQKIPDEALSMHAGQVADLVKCIEENRRPPTSGAEARRAIEFITGLYKSAMT
                     GEPVRRGTIGEDDPFYHRLNGAPR"
     gene            7581..8330
                     /locus_tag="VHM69_04665"
     CDS             7581..8330
                     /locus_tag="VHM69_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2739717.1"
                     /translation="MRLSVSEGMAYKTKQEFVYQTLRRAILRCELAPGQRLPTQEIAN
                     RLGVSLIPVREALQLLQSEGLVENRAHVGAAVARISGSSVEEVFTLLEGLEAVAVRVA
                     ARRMKPEAVEDLAVLLAEMDAAVENGEYELWGELNTRLHLSIARSTEMPVLRAMTEKV
                     FDLWYRVQRCYFTEVLVQRVLQSQQEHHAILRALRDRNEAELERLVKIHNRNAFKAYA
                     EHLSNGSAGSGEDRIIDDASKMREVGTETAS"
     gene            8380..10371
                     /locus_tag="VHM69_04670"
     CDS             8380..10371
                     /locus_tag="VHM69_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2739718.1"
                     /translation="MRHVRQILGVVVVLCVVTIVGCDFLSTEAESGGQNEQGGQGGGG
                     AGGSEEKGKEAPELAELVDAGDLPPVEERLPAQPMEVETVDSIGQYGGTWNSAILGTA
                     DVVWLWRTVSYEPLVRWDPEWTEPIPNVAENIEVADGGREYTFKLRKGIKWSDGKPFT
                     ADDIAFAYEDVILNEDLTPDVPTTFLGGDQPAEFEKIDDYTVKFTFPDEPNGLFLEQL
                     AYNGLITAWPRHYLEQFHEKYNPDVQQLAEEEDAADWIELFTAKAGLAGLVDPVHYQN
                     NDLPTITAWDVTQPLGEGGRVVLERNPYYWKVDPEGSQLPYIDRVEFDIIPENEAILQ
                     RTLEGSIDMHIRHINTFTNKPVLAQNRDSGGYDFFDATAANMNTMNIALNLTHEDPVK
                     REIFNNKDFRIGLSYALDRQEIIDVVFQEQGEPFQAAPRPDSPFYDEGLAKQYTEYSV
                     EKANQALDRAGYTERNSQGIRLGPDGEPISFTIEFATDFLPTWPDALELVQGYWEKVG
                     VDVQIKPVDRTIFYERKEANQPDATVWTGDGGGKDVTIEGRWYFPFSNESNYAIPWAE
                     WYVSRGESGQEPPEAAKRQMELYDKLHATLNEDEQAKIMGEILNIAEEEFYVIGTSLP
                     PESYGIVKNNFHNVPDMYDAALYPQPGPTNPEQYYITGE"
     gene            10394..11386
                     /locus_tag="VHM69_04675"
     CDS             10394..11386
                     /locus_tag="VHM69_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739719.1"
                     /translation="MVKYIVRRVLYMIPTLILISMISFAIIQLPPGDFLSTLVSQMSA
                     QGESVDQAQLEALENRYGLDEPFYVQYFKWISNIVLHGDFGESFEWNRPVSELLAERL
                     PLTIGLATATLLFTWAVSLPVGVYSAVRQYSIGDYIITFLGFLGIATPNFLLALVIAY
                     ISFKYFGQSVVGLFSPEFVDAEWNLGKLLDLLAHIWVPVFVLGAAGMAGIIRIVRANL
                     LDELHKPYVTTARSKGVAERTLLIKYPVRVALNPFVSTVGWVLPLLLSGEVIVAQVLG
                     LQTTGPLLLRALLSQDMYLAGSIILMISVLTVIGTLISDILLAWLDPRIRYQYQ"
     gene            11396..12541
                     /locus_tag="VHM69_04680"
     CDS             11396..12541
                     /locus_tag="VHM69_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739720.1"
                     /translation="MTDQMPPRVQEEAPVQAPDQKEAEIYVASQWKLVWWRFRKHRLA
                     VIGTVIVILIYMIALFAEFFAPFTPGSFDADYTYAPPQRLHFFDDDGFGLYVYGYSSK
                     LNEETLVRTFVVDESKKIPVRLFASGNEYEMWGLFKSDLHLLGPVEKGAPMYLLGAND
                     QGQDMLSRIVFGARVSMSIGLVGVAMSFVFGILLGGISGYYAGTTDTVIQRLIELLMS
                     IPTLPLWLGLSAALPPEWSPLRIYFGITIILSLIGWTDLARVVRGRFLSLRGEDFVMA
                     AELDGSSKPRIILRHMLPSFASHVIASLTLAVPAMILAETSLSFLGLGLQPPIVSWGV
                     LLQEAQNIRSVATAPWLLLPGLAVIIAVLALNFMGDGLRDSADPYAR"
     gene            12552..13646
                     /locus_tag="VHM69_04685"
     CDS             12552..13646
                     /locus_tag="VHM69_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739721.1"
                     /translation="MDPNEALLEVRGLKTHFFLDEGIVKAVDGVDLNIRRGETLCVVG
                     ESGCGKSVTARSILQLVDRPGRVVEGEILPPPPPNGEGKKNGAATDTRKVLDLASLDP
                     RSKAMRDIRGKEISMIFQEPMTSLSPVHTIGDQIMENVLLHLPMNKQEARERAVEELG
                     RVGIPNPERRLDAYPFQLSGGMRQRAMIAMALACNPSLLIADEPTTALDVTTQAQILE
                     LMRGLQEEMGMAIMFITHDLGVVAELADDVAVMYLGIVVERGDVDSIFHDPKHPYTQA
                     LLRSIPRMGRNTGQRLDSIRGMVPHPYNRPMGCPFHTRCDEFMEGYCERILPPTIPLE
                     GTSPESPRETRCLIYGGKDEEGAKGEGEAE"
     gene            13647..14696
                     /locus_tag="VHM69_04690"
     CDS             13647..14696
                     /locus_tag="VHM69_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2739722.1"
                     /translation="METKRNGDEALLELRDLKMHFPIKQGFLSRTVGHVKAVDDVDLK
                     IRRGETLGLVGESGCGKTTLGRCIMRAYEPTAGEILYRKKDGDTADLAKLGTGALKPY
                     RREIRMIFQDPNSSLNPRMTLLQIVGEPLKVNKIASGKDIEDRVAELLRRVGLRPEYM
                     SRYPHAFSGGERQRVSIARALALEPRFVVADEAVSALDVSVRAQILNLLQDLQEEFDL
                     TYLFISHDLSVVEHLCDRVAVMYVGKVVEVAETAEIFDNPKHPYTEALLSAVPKPDPR
                     LRDRAWRIVLKGDVPDPANPPSGCYFHTRCPYAEDRCKDEEPFLRELRPGHHAACHFS
                     EDLDLRGVGDQGAVR"
     gene            14717..15871
                     /locus_tag="VHM69_04695"
     CDS             14717..15871
                     /locus_tag="VHM69_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEX2739723.1"
                     /translation="MPKGEVFPAEWLSFADPATGVEVRQLTNHKGHSHHLYFTNPGWY
                     DGGRRLLFGSDRTNRTNLFGVDLESGEIIQLTDLDQPPPPAETSFLFTSVNPTREEAY
                     FWHGRRLVALDLGSLEERTLYEAPEGFLTNMTNVTADGKQVCVLVFEDLSHEFGVDLL
                     HGYVGFEEYWEAGPLSRIVRVGIEGGEAETVFEEDYWIGHANTSPTLPNLLTFCHEGP
                     WHRVDNRIWGLDLDTGRAWKIRPRSNREAVGHEYWLADGVRIGYHGTGINGEHFYGSI
                     RHDNTDRVEAPFPHGSTHFHSNDLDLIVGDGSRDAPRLLLWRFRDGRFEGPRVVLTHR
                     GSFHTQILHVHPRFSPDGNQILFASDASGYGNLHLVDTPDFDTLPDEGNA"
     gene            15868..17505
                     /locus_tag="VHM69_04700"
     CDS             15868..17505
                     /locus_tag="VHM69_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739724.1"
                     /translation="MNEEQYSEEHRMLGVSWAGPDYHTRVPQGAWVHPTRESLDYALN
                     LLKSESQERAAAVVRKVLELQDTDPTSETYGIWPWLLEEPLSEMDPPDWNWADFCGAR
                     LAQMLVEHAGVLPGDLIRDTRTALGHAAATIFRRNVGPAYTNIAVMGAGVTLAAGELL
                     DEPRLSDYGRRRLRNIVEHTEHHGGFNEYNSPTYTVVTLSECERMLQLVRDPSARADA
                     EMLRLLAWRTIAERYHPGTHQWSGPHSRAYSDRLDAGISGFLSERTGVEVRPHPKAGL
                     RDRFEGTAPLPCPSGLVDRFRSPPHGETELRSTFIRRAGEGDSIQGTTWMSEDACLGS
                     VSRDNLWDQRHARLGYWRTEEDPAVVLRLRFLHDGRDFSSACARNAQSGPRILSAIGL
                     LTGMGDAHHQLDRTADGAFDAEDFRVRYELVGEGVAGRALEGSRFELSAGSCQAIVHT
                     APGRFGPHELVWEMERAEGRVCLDGVCYRGPRRRFEPVELGDVVIFAGLELLRADQPP
                     SASGPAACEPDGGMVDVSWPVAGGLRILAPTRAQPYP"
     gene            17519..19219
                     /locus_tag="VHM69_04705"
     CDS             17519..19219
                     /locus_tag="VHM69_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX2739725.1"
                     /translation="MPGRFRRHDRRPVTQLNGIWDFAFLGDEDPDAVDAASIGFDDVM
                     AVPGCFDATPRYAGRRGLAAYRTRAFLPEHGRYRLILDGVHHWCRVFVNGEKMRDHVG
                     GFTRFCVDFTGRVDEEVEIVVLVDNRFDYDRCPLHLEYFDWYHFGGIARPVELHRLAD
                     PWIEGLTIITEAVESRRVAVTIDYDATAPPAKTDLTITCDGRVVLDETVDLGQTPGRI
                     ERVLELPGTRLWSPDEPNLHVIQATLGEDDARERIGIRQISVEGRSLLVNGEPVRLLG
                     FNRHEAHPQFGHFQPEALLVSDVQQLRDAGCNFVRGSHYPQDVRFLDLCDEAGICVWQ
                     EAIGWQHTTEHLTDETFVRAQLTNIEEMVAASANRPSVILWGILNESESHDPACRLAY
                     ERLLGRLRELDPTRPVTYASNHPFDDACLDLADVVSINSYPGWYFGGLEDVPDELDRI
                     SEHLDSVGQGDEPLIISEIGAGAIPGWRDPHAARWTERYQAELLDTVIRHLFVDRDRA
                     CGLAIWLYNDFRTTEEIGRALGRPRGFNDKGVVDEYRRPKLAYETVRRRFLTLTDDGD
                     "
     gene            19206..21065
                     /locus_tag="VHM69_04710"
     CDS             19206..21065
                     /locus_tag="VHM69_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX2739726.1"
                     /translation="MTETRETMLGRHDPASIERALNEASPSPPFPPASDRAAWDEVRE
                     VIGEERVSELFDRASANTDVPALPATLYLEFARNGSREGYERPANRRREMLSDLALAE
                     CLEGEGRFLDPLLDVAWAICEESSWSWPAHQGDLAVMDHPVIDLSAAMTALYLAELDL
                     LLGDRLDPVLGRRIRHEVDRRCLAPYMRRHDHWWLFDSPERPVNNWTAVCNAGVAGAA
                     IHLETDAARLAEILARAARSLDDYLSTFDEDGGSSEGPAYWSYGFGYFTVLAHLVGHR
                     TGWKVDIFEGEHVRRVARFPSRTRLGPGLYVNFSDCDRHISFIPAHLSYLSRWLGLPD
                     LARLAREQTGDVERERELTWSLRNLFWRPEPVEGHFVPARHDWFGGMMWMISRQDPDD
                     PDALVLATKGGHNGEMHNQNDVGNVIVHVGAESVIADIGRGRYTKAYFGPERYEHIAN
                     SSQGHSVPVPNGQEQLPGGEHGARLLQHRADDPADLAAFELRDAYPTEADLDSLKRTA
                     VLHRGPPAWVELTDEVRFTSEPGSFESPLITFGEAEVSPDSVLLRGERGTLRVYFDPE
                     SVVPRVEVVEGVDLAEGPADVRRVVFALQERVQEGSVHLRIEPEKSSSGSDPG"
     gene            complement(21094..22854)
                     /locus_tag="VHM69_04715"
     CDS             complement(21094..22854)
                     /locus_tag="VHM69_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739727.1"
                     /translation="MRKDVDKGSGRSLDTPDAAEAVHPVEILRDAVESYAPHLQPSGE
                     LHDPVFGEPTQYGTPYHALCNAVLAMGDGGERRSAYVDRAVRGLKVALDHVSDPGLPP
                     TASGFDPATGAVTRSNHRDFFWPPIIKTFRILRSMRVDGTEVFARRISGVDIERAFAK
                     RPPSNWASVWLSGEWLRLREGLSPFSKEDIDLWLDAFFDAHILTEQGLYQEPGHPNSY
                     DLFTRYHLADMLMEGYDGRRREDLERLIRTGLRRSLGVQLSDGSLASAHRSTGQTWTL
                     GAQCAYFTHVANHFRGKDPELARHADEAAYLALSSFRRWQRRDGPYSPVENLLPPGYR
                     VGYERYTADGHYANLAAAFLAIAVSNGLDLPVTQPSGERESSVWLEHDPTYRAVLHNG
                     AYSLHVNARPAPDYDAFGLVDLTFGPGRFLHFASVAKHLESGKFINLGMAGSTGPASQ
                     GDLMLIGRIETDWTPAGLRLEARGSGSPYRYRLAARVETDGVYVEEATPGLEEPKALL
                     VPYLRDPGTGTFTEARVQAGGVCLIHGREQVEISYDAPVKRVVHLTGGFQSRRGLCGL
                     LRIEFRHKSEGISYRIRAIH"
BASE COUNT         4197 a         7592 c         7242 g         3934 t
ORIGIN      
        1 ctctggtcac gcgccactcg acgccgtcgt agccggcatc ctttaactcg cgcacagcct
       61 cctcgggcgt gaggtcaggc aggccgactg taaagactcc tactttcata gaccctctct
      121 tcggtcgtct ggtctacgtc ctttcgggct tcgtttgctc aggccatcac ggctgccctc
      181 tcctttgtga tctcgtgctc caacggctcc cccgccacgt accggcgaag ctcgtccacg
      241 acgagtcggc ccatgcgccg acactcgtca ccttgagagc ctgcgatgtg gggtgtgagc
      301 acgacgttgg acaggtcgta aaggggagag tccggttccg gcggttcagg atgcgtcacg
      361 tcaagaacag ccgtgaggtc cggccggtcc ttcagaacgg agaccatctc gccttccctg
      421 accacggccc ctcgcgaggt gttgacgagc gtcgcgcctt gcttcatcga ggcgaggtgt
      481 gagccccgga tcataccctc cgtctcaggc aaccacggcg tgtgcaacga gaccacgtcc
      541 gcctccatga agagcggatc caacgtcatc agctcgacgc caagttcggc cgcctcctcg
      601 attgtcacgt atgggtcgtg ggcaaccacc cgcacgtcga aaggccgaag cctctcgcgc
      661 agcatccgac cggtcgcacc gagggagatg atccccacgg tgctcccgta ggcaccgggc
      721 attgcggaga ccgtggggaa gctgcgctcc tgttttacgg aggcggccaa gcgccagccg
      781 cgcttgaggg agaaaagtat ggcggccagc gcgtactccg agacggggac ggcgtttgcc
      841 gagtaggcgc tgactacccg cacatttctc ttccagagtg cgtccgtcac gaacgagcgg
      901 acggaacccg cgccgtagaa gacggctctg agatcgccgg cagcctcaag gaatgctccg
      961 tcgaataccg gcgcccccca cccggagagt atgacctcca ccccgtccaa cacgtcgagg
     1021 tttttcgcga cggtttcggc cgtctgcgga acgtcttcga cttcgacgag ggcggctatg
     1081 tcggcccgct cgggctcgcc gtaaatcctg tcgaaggcgt cggaggcaag cacgtagagg
     1141 ccgcggggtc tcacgcgtga acaccagcca agacgtgttc aaggtagcgg cgttttaccg
     1201 tttcgaggac ctcctcgcca ggcgtcgccc agaccgcctc gttgaagatc tcgacctcta
     1261 tcggaccctc gtagtctgcg gcatcgatcg ccgctcgcat ccgccgcagt tcgatgacac
     1321 cgtcgcccat cagggcgcga gagaggagtg ggtccccgcc tccctttggc cagtcgtcca
     1381 cgtggaaacc gacgatggcg cccgcggcgc gccggatctc gtcgtacaac gcgggatccc
     1441 accacacgtg gtaggcgtcc acgacaaccc ctaccccggg cgacccgacc cgcctgacga
     1501 tgcccgtcgc ctcgcccagg gtgcacacga cggagcggtc ggcggcgaag gcagggtgca
     1561 ggggctccac gccaagaatg accccggcat cgcttgcgta cggcgcgatg gcttccacgc
     1621 cgtccagcac gttgcccctg gcagccccga tatcccggtc gggagccccg ccgcacacaa
     1681 gcacgagcac ttccgcgccg agggccgcgg cttcgtccac ggcgcggcga ttgtcgtcga
     1741 ggcgcgcgag ccgctcccgc tcggtggcgg cgggaaacat gccgccacgg cacaggctgg
     1801 acaccttgag ccccatctca cgcacgatgc gggcgctctt ctccaggccg gtctcggaga
     1861 ccttgtcgcg ccacaggccg atccagggta tcccggccct cgcgcagccc tcggccgcct
     1921 cgcgcacgcc ccactgtctg gtcgtggcct ggtttaggct gaggcgcgag aggtccccac
     1981 tcaagagaca cccgccaggt cgagaacggg ccgcatcctc cctgcggccc gctccgggtc
     2041 ggccaggagc cccgccttgt ccgcgagcac aaaaagctgg gagaggtgga cgacggagcg
     2101 tgccccctcc tgtccaccga ccatgcggaa gtgatcctgg tgtccgttga ggtaggcgag
     2161 gaagacgacg cccgtcttgt agaagcgggt cggtgccttg aagacgtggc ggctaaaggg
     2221 aacggttggg gcgagtaact cctcgtagcg atccgtgtcg cccgcgtcga gagcgtgcag
     2281 cgcggccgag gcggcgggcg cgatggcgtc gaagatgccg aggaaggcgt gggagaagcc
     2341 ttcatcgtcg ccccgtatga gttcgggata attgaagtcg tcgcctgtgt acatcctgac
     2401 gccttcgggg agactgcggc gcatctcgat ctcgcgctcc gcgtcgagca gggagacctt
     2461 tatgccatcc accttcgacg catgttccgc gatgatggcg aggcaagact ccatcgcgag
     2521 atctaggtcg tcgtgacccc agtatcccgc gagggccggg tcgaagtcct ccccgagcca
     2581 gtgcagaatg gcgggctcgg agagccgcga gagcacctcc ccgtagacac tggcgtagtc
     2641 ttcggggcca cgggcggcgg cggcgagcgc ccggctcgcc atgaggatga cgcgcccacc
     2701 cgctcgctcg acggaggcgc actgctcctc gtatgccgag gacacgtctc gcagcgtgac
     2761 ggatggggac attcccaggt ggtcagtgcc ggctccgcag gcaatgcggt ctccggcgcc
     2821 ctccgctcgg gcctcggcga tggagcgatg gatcagctcc tctgccgtcc cccagtcgag
     2881 gcccattccc cgctgggcgg tgtccatcgc ctcagccacg gagaagccga gggaccacag
     2941 gtacctccgg taggcgagcg tggcctccca gtcgagggcg acgtcgagcg tcgggtcgtt
     3001 gtcggcaagg gggtcagcga cgacgtggac cgcagcgtaa gccacgcgtg tccacggcgg
     3061 cgcgacggac actacaggca aaggctcccc ctgcgggacg tattgcatga gcgacccgtc
     3121 ggagcgtgga aggatcagtt ctttaagcat gatcaagctc ccgcgtccgg cctgcgtcga
     3181 gagccggcac gtccagccag cgtctctccc tcgaggaacg cagccccagc tcggcgagct
     3241 ggacgcccct ggcgccctcc cagaggtcgt atgggaacgg ctcgtcggca acaacgtgtc
     3301 tcaggaacat ctcccactgg actttgaagg cgttgtcggg atcttcgact tgcggtacgt
     3361 caagccactg cgaacggtag ccgttcgtgt cgggcaggtc ggggttccag acggccttcg
     3421 gggtgttcgc gccgtgctgg gccttgcact ctcgcaggcc ggccaccgcg ctcccatcgg
     3481 tgccgtccac ctggagctcg aatagctcgt cgcggttgac gcgcacgcac cacgaggagt
     3541 tgatgttggc cacgataccg ccgtcgagct cgaaggtggc gtaggccgcg tcgtgggccg
     3601 tcgcaacgta gggctcgccg ttctcgtccc aacgcttcgg cacgtgcgtc gcaccgaggc
     3661 aagagacgga tctgacctcg ccgaataggt tatccagtac ataacgccag tggcagagca
     3721 tatcgaggat gatcccgccg ccgtcctcct cgcggtagtt ccaggagggc cgctgggacg
     3781 gacggccgtc gcccgggaag acccaatatc cgaactcgcc acggacggag aggatccttc
     3841 cgaaaaaacc gctgtccacc agacgcttga gcttcatcat gccaggaaga aagagcttgt
     3901 cctgcacgac gccgttcttt actctcccct cgccggcaag gcgagctatc tcaatcgcct
     3961 cttcgacagt cgtcgccgta ggcttctcgc agtagacgtg cttacccgcc ccgatagccg
     4021 ccttgacgct ctcagtcctg cgggtggtgg tctgggaatc gaagtagatc tctacctcgg
     4081 ggtcggagag acacttgtcc aggtcggtgg tccaccgctc gatgccatgc gcctccgcca
     4141 ggcgtcgcag ttttgtctcg tcacggccga cgaggatggg ttcaggtacg accacgtcgc
     4201 ctccgggcaa cgtgaccccg ccctgttccc tgatggcgac cagggagcgc acgagatgct
     4261 ggcgggttcc catcctccct gtgaccccat tcattgcgac ggttacggta cgcatgacct
     4321 catcactcct tccccacgcg gctgtgtatg ctgtccacgt actcttctat ccgctcgcga
     4381 gaccaccggc cgtcgcccaa cacgacgcgg tagtcgaggg caagctcctc ctcgggttcc
     4441 agcacgtact cttcatcgaa catgaacgag caactcgcgc aggcgtaggg atcgttgcgc
     4501 acgaaccatt tgccagggaa acgcacgttc gtcggggaat ccagaaatag tactgtggac
     4561 gcgcgcccgg ttccgtcatg gagcccgata taagccagcc atggggaggt ccggcccatc
     4621 gtctccggtc cttccagccc gtccgcggcg agtatctcgc cgtcgtcgaa ggagcgcggc
     4681 ccccgccaga acagcccgcc gtagccagcc gccggccttc cctccgtggt tggcgagccg
     4741 aaagtcaggg agcgctccgt gacgttcttc agccggaaag agaggtccag gctccagaag
     4801 ccctccgaag ggtagatccc ccccaccgag atcccccgct cctcctcgat ccaggtttct
     4861 cccccgtggg agatccagga caggcgttct ttgaggaatg agccttcacc acgtatctca
     4921 caccagtcgt cgtgccgtat catcccctgg ttttcgagcc actggtatcc cttgccccgc
     4981 acgtacgtcg gaccgcccca gaagttctcg cctgagaggt acgctgaggt catcgccagt
     5041 cccgtatgcc aggggtgatc gtggggtctg aagagcgtca cctcttcccc cgccagggtg
     5101 cgcaaaggat ggaagtatgg cttgggagat tccgaggcat ctactctggc ctcgtagacg
     5161 taccggaaca ggaggtcccg gccgtctctt gcctcgaccg actcacccag cgcgtgcatt
     5221 agccgaagag tcaccgttct ccttctttcg cgagaaagtc ccgcagcgcg tcctcatccg
     5281 gccgcactcc gatgcccgcg tccgccggca ggtggacggt gccgcctgat tcgacttttg
     5341 ccttgtcggg gaagatggcc gactcgtcgg tgaactggct gccgttgagg gcggccggac
     5401 ccgcgcagcc gaaggccgcg gcgacctggc agacggccac cttcgtgagg agaccgtcgg
     5461 tgaggccgct cacgagcagc ggcaacccgg cagcctcggc cacggagatc tgggcgagcg
     5521 tcggccacac tccgccgctg cgcgtcacct tgacgacgag gtaatccacc aacccctctg
     5581 cggcgtgcgc gaagaagtct gccgggctca cgatcgactc gtccaccgcg agcggtatct
     5641 ggacgcttct cctcagctcc cgcatgaggt gtatgtggtc ggcgggcagg ggctgctcga
     5701 gcacgtccac acccgctccg gcgaggcgtg gcgccgccgt acgcgcctcg tggagcccgt
     5761 agccctggtt ggcgtcggcc cagacgaaag cctcgggccc cgccgcttcg cgcacgcagc
     5821 tggcgacctc gagatcggcg cccgatcccg ccttgaagtt gaagtgtctg aacccgtcct
     5881 gccgcgcagc gtccacatcg tcccttacgg tcgcggcgtc tcgccctgtc accgtgtagc
     5941 tcaactccac cgaggcacgt tcgtcggcgc cgccgaggaa agaccgcaag gacatccccg
     6001 ccgccctggc gcacaggtcg tggaccgcca ggtcaacggc cgccttcgcg acgggttgcc
     6061 ctgtgctcgg gccgcgacct atggccgcgt gcatccggcg gtgcaatccc cacctgtcgg
     6121 cgaccggcaa cccgagcagt gggggggcaa ggtagttgcg aatggtcgtg gatactgtct
     6181 ccagcgtctc gtaggaccac tgcgggacgg gacgaccctc gccccagccg acctctccct
     6241 cgctgtccgt gaccttgacg aagacgtgcg gcgccttccc gccggccttg cccgccgacc
     6301 ctgatgcgaa ctggaaggtc tcgatcaccg gcaggagcac cgggaagacc tcgatgtttt
     6361 cgatcttcct catctcggcg cgccgttcag ccgatggtag aagggatcat cttctcctat
     6421 cgtgccgcgg cggactggct cgccggtcat ggcggacttg taaaggccgg tgatgaactc
     6481 gatcgcccgt cttgcctctg ccccgctggt cggcggccgc cggttctctt cgatgcactt
     6541 caccaggtca gccacctgcc cggcgtgcat ggagagggct tcgtcgggga tcttctgcca
     6601 ccgggcgaga gagtccgcgt ggaccgcgcc atcgggtatg ctgtagcgcc agtcgctgtt
     6661 cgagtaaccg tagaggctgt ccacctccac ggtggcttcc tggaagtcga atcgcaggtg
     6721 ggttctctgg cgcggggaga gcacgctgtt gacaacgctt cccatggccc cgttctcgaa
     6781 ccgcacgacc gccatcgaga cgtcctcgac ctcgatatcc cggtcgagcg tgccagccat
     6841 cgcgcgcacc tcatgccagt cgccaagcat ccacaggaac aggtcgatcg cgtgaatgcc
     6901 ctggcccatc gtggcgccgc cgagctccgt ctcccacttc ccgcgccagg gcacctgata
     6961 gtaggccgcg tccctgtacc aagtggtctg gcacaatccg accagcgcac gtcccaattc
     7021 accagaggag atcaaagcct tcaggtgccg agcccccgca ccgaaccgcc actggtatac
     7081 gctgctgcag tacttcccag tcgccctctc ggcggcctcg atctcgtcca tctcctcaag
     7141 cgacgcgcac aagggcttct cgcacagcac ccaggctccg gcctccatac actggatgct
     7201 caactcccga tgcgactgcg ggggcgtgca tacgtgtacc agatccggcg cctcctcctc
     7261 caacatctgc tggggatcca cgtatgaccg cggaatgccg tgctcagagc agaacgcagc
     7321 cgccaacgcg gagtccacgt ctaccgccgc gaccaactcc accctcttgc caaccttccc
     7381 cagggccgcg acgtgcgccc tggcgatgtt ccccgtacca atgatcgcag cccggatctt
     7441 cctcactccg acccccgcct cccggaagcc cggccttttc ctatctcgtc cccaatatcg
     7501 tgcaccagct tgacgcctcc tgtccgacat tatataatat atattaagaa agtgaaggaa
     7561 gggggaagga aggtatcggg atgcggctct cggtctccga ggggatggca tacaagacga
     7621 agcaagagtt cgtctaccag accctgcggc gggccatatt gcgttgtgag ctggctccgg
     7681 gtcaaaggtt gccaacacaa gagatcgcga accggcttgg cgtcagcctc ataccggtaa
     7741 gggaagcgct gcaattactg cagtccgagg gtctggtgga gaacagggca cacgtggggg
     7801 ctgcggtcgc ccgaatatcc ggctcctctg tcgaggaggt cttcacgttg ctggagggat
     7861 tagaggccgt tgctgtaaga gtggcggcgc ggcggatgaa gcccgaggcg gtggaggatc
     7921 tggcggtgct actggccgag atggacgctg ccgtagagaa cggcgagtac gagctctggg
     7981 gagagctcaa cacgaggctg cacttgtcca tagctcgcag tacggagatg cctgtgttgc
     8041 gggcgatgac cgagaaggtg ttcgacctgt ggtacagggt gcagcgttgc tacttcacgg
     8101 aggtactggt acagcgcgtc ctgcaatccc agcaagagca ccatgccata ttgcgcgccc
     8161 tgcgggatcg taacgaggca gaactggagc ggttggtcaa gatccacaat cggaacgctt
     8221 tcaaagctta cgcggagcat ctgtcgaacg gttcggcggg ctccggcgaa gatcggatca
     8281 tagacgatgc gagcaagatg cgagaggtgg ggaccgagac ggcatcatag cttgcccggc
     8341 gtggggacgt cggatcttgt ccaaaggaaa ggggtaggtg tgaggcatgt aaggcagatt
     8401 ctgggtgtgg tcgtggtcct gtgtgtcgta accatcgtag ggtgcgactt tctctcgacg
     8461 gaagcggagt ccggcgggca gaacgagcag gggggtcagg gtggcggcgg cgccggtggg
     8521 tctgaggaga agggcaaaga agcgccggag ttggccgagc tcgtagacgc cggggacctt
     8581 ccccctgtcg aggagcggct accagcgcag ccgatggagg tcgagacggt cgacagcatc
     8641 ggccagtacg gcggcacctg gaactcggcc atactcggca ccgccgacgt cgtgtggctc
     8701 tggcgcaccg tgtcgtacga gccgctcgta cgctgggatc cagagtggac cgagcctatc
     8761 cccaacgtcg cagagaacat cgaggtcgcc gatggcggca gggagtacac cttcaagcta
     8821 aggaagggca taaagtggtc cgacggcaag cccttcacgg ccgacgacat cgccttcgcc
     8881 tacgaagacg tcatcctcaa cgaggacctc accccggacg ttcccactac cttcctcggc
     8941 ggagaccagc ctgctgagtt cgagaaaatc gacgactata ccgtcaagtt caccttcccc
     9001 gacgagccca acgggctctt cctcgagcag ctcgcataca acggactcat caccgcctgg
     9061 ccgcgccact accttgaaca gtttcacgag aagtacaacc ctgatgtaca gcaactcgcc
     9121 gaagaagagg acgccgctga ctggatcgag ctcttcaccg ccaaggctgg gttagctggc
     9181 ctggtcgacc ctgtccacta ccagaacaac gatctgccga ccatcacggc ctgggacgtc
     9241 acccagccgt tgggcgaggg cggcagggtc gtcttagagc gtaacccgta ttactggaag
     9301 gtggatcccg agggcagcca gctgccatac atagaccggg tcgaattcga catcataccg
     9361 gagaacgagg cgatccttca gaggacgctc gaaggcagca tagatatgca catccgccat
     9421 atcaacacct tcaccaacaa gcccgttctg gcacagaaca gggacagcgg cggctacgat
     9481 ttcttcgacg ccacggccgc caacatgaac accatgaaca tcgccctcaa cctcacgcac
     9541 gaggatcccg tgaagcgtga gatcttcaac aacaaggact tccgcatcgg gctctcctac
     9601 gcgctagacc gccaggagat aatcgacgtc gtcttccagg aacagggcga gccattccag
     9661 gctgccccgc gcccggattc accgttctac gacgagggac tcgccaagca gtacaccgag
     9721 tactcggtgg agaaggcgaa ccaggccctc gaccgcgccg ggtacacgga gcgcaactca
     9781 caaggcatca ggcttggtcc cgacggcgag cccatctcct tcactataga gttcgcaacc
     9841 gacttcttgc ccacctggcc agacgccctc gagctcgtgc agggatactg ggagaaagtc
     9901 ggggtcgacg tgcagatcaa gccggtggac cgcacgatct tctacgagcg caaggaagcc
     9961 aaccagcccg acgcgacggt gtggaccggg gacggcggcg ggaaggacgt caccattgaa
    10021 ggaagatggt acttcccgtt cagcaacgag tccaactacg ccataccgtg ggcggagtgg
    10081 tacgtcagca gaggggagag cggacaggag cctcccgagg cggccaagcg ccagatggaa
    10141 ctgtacgaca agctccacgc cacgctcaac gaggacgaac aggccaagat catgggcgaa
    10201 atcctgaata tagccgagga ggagttctac gtcataggca cgagcctgcc gcccgagagc
    10261 tacggaatcg tcaagaacaa cttccacaac gtgcctgaca tgtacgacgc cgcactgtac
    10321 ccgcagccgg ggccaaccaa cccggagcag tactacatca ccggtgagta gggtcggcag
    10381 gagggagtag gacatggtca agtacatcgt gcggcgggtg ctttacatga tcccgacgct
    10441 catcctgatc tcgatgatct ccttcgccat catccagctc ccccccggcg acttcctgtc
    10501 gacgctggtt tcccagatgt ccgcccaggg cgagtcggtg gaccaggcgc agctcgaggc
    10561 gctggagaac cgctacggac tggacgagcc gttctacgtc cagtacttca agtggatctc
    10621 gaacatcgtt ctgcacggcg acttcgggga gtcattcgag tggaaccgtc ccgtatccga
    10681 gttgctcgcc gagagactgc cgctgaccat aggtctcgcc acggccacgt tgctgtttac
    10741 ctgggccgtg tccctgcccg tcggcgtcta cagcgccgtc cggcagtact cgattggcga
    10801 ctacataatc acgttcctcg ggttcctggg aatcgccaca ccaaacttcc tgctggcgct
    10861 ggtgatcgca tacatcagct tcaagtattt cggccagagc gtggtcggac tcttctcgcc
    10921 ggagttcgtg gacgccgagt ggaacctggg caagttactc gacctgctgg cacacatctg
    10981 ggtgcccgtg ttcgtgctcg gcgcggccgg tatggcgggg atcatccgca tcgtgcgggc
    11041 gaacctgttg gacgagctgc acaagccgta cgtgacaacc gcccgctcca aaggggtcgc
    11101 cgagcgcacg ctcctgatca aatacccagt gcgggtggcc ctcaacccct tcgtcagcac
    11161 ggtggggtgg gtgctgccgc tgcttctctc cggagaggtg atagtggcgc aggtccttgg
    11221 cctgcagacg acggggccgc tgctcctgcg ggcactcctg agccaggaca tgtacctggc
    11281 gggcagcatc atcctcatga taagcgtgct gacggtcata gggaccctga tatccgacat
    11341 cctgctggca tggttggatc cccgcatccg ctaccagtac caataaggag gagtgatgac
    11401 ggatcagatg ccgccgcggg ttcaggagga agccccggtg caggcacccg accaaaagga
    11461 ggccgaaatc tatgtcgcct cccagtggaa gctggtgtgg tggcgcttcc gcaagcaccg
    11521 gctggccgtc atcggcacgg tgatagtgat tctgatctac atgatcgccc tcttcgctga
    11581 gttcttcgcc ccgttcaccc ctgggtcctt cgacgccgat tacacctacg ccccacccca
    11641 gaggctgcac ttcttcgacg acgacggttt cggactctac gtctacggct acagctcgaa
    11701 gctgaacgag gagacgctcg ttcgcacgtt cgtcgttgac gagtccaaga agatccctgt
    11761 cagactgttc gccagtggga acgagtatga gatgtgggga cttttcaagt ccgacctcca
    11821 cctgcttggt ccggtggaga agggcgcgcc gatgtacctc ctcggggcca acgaccaggg
    11881 gcaggacatg ttgagccgca tcgtcttcgg cgcccgcgtc tcgatgtcca taggcctggt
    11941 cggggtggca atgagcttcg tgttcggcat actcctcggc ggcatctccg gctactacgc
    12001 ggggacgacg gacaccgtga tacagcgcct gatcgagctg ctcatgtcca taccgacgct
    12061 gccattatgg ctcggactct ccgccgcgtt gccgccggaa tggagcccct tgaggatcta
    12121 cttcgggata acgatcatcc tctccctgat aggctggacg gaccttgcca gggtcgtcag
    12181 gggacgcttc ctctccttga ggggtgagga cttcgttatg gccgcggagc tcgacggctc
    12241 gagcaagccg cgtatcatcc tgcgccacat gctcccttcc ttcgcgagcc acgtcatcgc
    12301 gtcactgacc ctggccgttc ccgccatgat cctcgccgag acgtccctct cgttccttgg
    12361 gcttggtctt cagccaccga tcgtaagctg gggcgtgctc ttgcaggagg cccagaacat
    12421 acgctccgta gcaacggctc cctggcttct gctgcccggg ctcgccgtga taatcgcggt
    12481 cctggccctg aacttcatgg gtgacggcct gagggactcg gccgatccct atgcccggta
    12541 agaaggcgag tatggacccg aacgaagcgc tgctggaagt cagggggctc aagactcact
    12601 tcttcctgga cgagggtatc gtaaaggccg tggacggcgt cgacctcaac atccggcgcg
    12661 gggagacact gtgcgtggtc ggggagagcg gctgcggcaa aagtgtcaca gcccgctcca
    12721 tcctgcaact agtggaccgc ccagggcgcg tcgtcgaggg tgagatcctg cccccccccc
    12781 cccccaacgg tgagggtaag aagaacggag ccgcgaccga tacacggaaa gtccttgacc
    12841 tcgcatccct tgaccccaga agcaaggcca tgcgcgacat ccgcggcaag gagatctcca
    12901 tgatcttcca ggagccgatg acgtccctaa gcccagtgca cacgatcggc gaccagataa
    12961 tggagaacgt gctgctccac ctgcctatga acaagcagga ggcacgcgag cgggcggtgg
    13021 aggagctcgg acgcgtcggc atccccaatc ccgaaaggag gttggacgcc tacccgttcc
    13081 agctctccgg cggcatgcgc cagagggcca tgatcgcgat ggccctcgcc tgcaacccga
    13141 gcctgctgat agccgacgag ccgaccacgg ccctcgacgt gaccacccag gcccagatcc
    13201 tggagcttat gcgcgggctg caggaggaga tgggcatggc gatcatgttc ataacccacg
    13261 acctcggcgt ggtcgccgag ttggccgacg atgtggcggt gatgtacctt gggatagtgg
    13321 tcgagcgcgg ggacgtagac tccatcttcc acgatcccaa acacccttac acccaggcgc
    13381 tgttgcgctc gatcccgagg atgggccgca acacgggtca gaggcttgac tctatccgcg
    13441 ggatggtgcc ccatccctac aaccgcccca tgggctgccc cttccacacc cgctgcgacg
    13501 agttcatgga gggctactgc gagaggatcc tgcctcccac gatccccctg gaaggaacct
    13561 cgccggagag tcccagggag acccgttgcc tgatctacgg tgggaaagac gaggaaggag
    13621 caaagggcga gggggaggcg gagtaaatgg agacaaagcg caacggggac gaagccctgc
    13681 tggagttgag agacctcaag atgcacttcc ctataaagca gggcttcttg agcaggacag
    13741 tgggccacgt aaaggcggtt gacgacgttg acctcaagat ccggcgcggg gagactctgg
    13801 ggcttgtcgg agagagcggc tgcggcaaga cgaccctggg tcgttgcatc atgagggcct
    13861 acgagcccac cgctggcgag atcctctacc gcaagaagga cggggacacc gcggacctcg
    13921 cgaagctcgg aaccggcgca ctcaagccct accgcaggga gatccggatg atcttccagg
    13981 acccgaactc ctccctcaac ccgcgcatga cgctgctcca gatcgtcggc gagccgctca
    14041 aggtcaacaa gatcgcctcg gggaaggata tcgaggacag ggtggctgag ctcctgcgca
    14101 gggttggcct taggcccgag tacatgtccc gctacccgca cgccttctcc ggcggcgagc
    14161 gccagcgtgt cagcatagcc cgcgcgcttg ccctcgaacc ccgcttcgtg gtggccgacg
    14221 aggctgtgag cgccctcgac gtctcggtga gggcccagat cctcaacctc cttcaggact
    14281 tgcaggagga gttcgacctc acctacctgt tcatctccca cgacctctcc gtcgtcgagc
    14341 acctctgcga cagggtggcg gtaatgtacg tagggaaggt ggtcgaggtt gccgagactg
    14401 cggagatctt cgacaacccg aagcatccct acaccgaagc actgctctcg gcggtgccca
    14461 agccggaccc gcggctcagg gaccgagcgt ggcgcatcgt gctcaaagga gacgtgccag
    14521 acccggcgaa cccgccatcg ggctgctact tccacacccg ttgcccatac gccgaagacc
    14581 gctgcaagga cgaagaacct ttcctgcgcg agctacgacc aggccaccac gccgcatgtc
    14641 atttctccga ggacctcgac ctgaggggcg tgggcgacca gggggccgtg cgatagacga
    14701 accagaggag gctctgttgc cgaagggcga ggtcttcccc gcggagtggc tctcgttcgc
    14761 tgaccccgca accggcgtgg aggtcaggca actcaccaac cacaagggcc acagccacca
    14821 tctctacttc accaaccccg gctggtacga cggcgggcgc aggctcctct tcggctccga
    14881 caggaccaac cggaccaacc tgttcggcgt ggacctcgag agcggggaga tcatccagct
    14941 aacggacctc gaccagcccc cgccgcctgc cgagacctcg ttcctcttca ccagcgtcaa
    15001 cccgacgcgc gaggaggcgt acttctggca cggccgcagg ctcgtcgccc tggacctggg
    15061 atcgctcgag gagcgcacgc tatacgaggc gccggaaggc ttcttgacca acatgaccaa
    15121 cgtgaccgcc gacggcaagc aagtctgcgt cctcgtcttc gaggatctct cacacgagtt
    15181 cggggtggac ctgctgcacg ggtacgtcgg gttcgaggaa tactgggagg cggggccgct
    15241 atcgcggatc gtccgtgtcg gcatcgaggg cggggaggca gagacagtgt tcgaggagga
    15301 ctactggatc gggcacgcca acacctcccc cacgctgccg aacctcctga ccttctgcca
    15361 cgaaggccct tggcacaggg tggacaaccg catctggggc ctcgacctgg ataccggccg
    15421 cgcctggaag atacggccac gctctaatag agaggccgta ggccacgaat actggctcgc
    15481 cgacggggtg cgtatcggct accacggcac cggcattaac ggcgaacact tttacgggag
    15541 catccgccac gacaacacgg acagggtgga ggcccccttc ccccacggct ccacgcactt
    15601 ccatagcaac gatctcgacc tgatcgtcgg cgacggtagc cgcgatgcgc cgcgactcct
    15661 gctctggcgc tttcgcgacg gacgcttcga gggaccgcgg gtcgtgctca cgcaccgggg
    15721 ctcgttccac acccagatcc tgcacgtcca ccctcgcttt tcacccgacg gaaaccagat
    15781 acttttcgcg agcgacgcga gtggttacgg caacctccac ctggtagaca cgccggactt
    15841 cgacaccctg cctgatgaag ggaacgcgtg aacgaggagc agtacagtga ggagcacagg
    15901 atgctcggcg tctcgtgggc ggggccggac taccacacgc gcgtcccgca gggcgcctgg
    15961 gtgcatccga cgcgcgagtc gctggactac gcgctgaacc tcctcaaatc ggaaagtcag
    16021 gagcgggcgg cggccgtagt ccgaaaagtt ctggagctac aggacaccga cccgacgagc
    16081 gagacctacg gcatctggcc gtggctcctc gaggagccgc tctccgagat ggacccgccg
    16141 gactggaact gggcggactt ttgcggcgcc aggctggccc agatgctcgt cgagcacgcc
    16201 ggggtgttgc ccggagacct gatccgagac acgcgaacag ccctcggcca cgctgcggcc
    16261 actatcttcc gccgcaacgt cgggcccgcg tacacgaaca tcgccgtgat gggcgcaggc
    16321 gttacgctcg ccgccggaga gctgctcgac gagccgcgac tctcggatta cggacgccgt
    16381 cgcctgcgca acatcgtcga gcacaccgag caccacggcg ggttcaacga gtacaacagc
    16441 ccgacctaca ccgtcgtcac cctgtccgag tgcgagcgga tgctccaact cgtccgggac
    16501 ccttccgccc gcgcagacgc cgagatgttg cgcctcctgg cctggcggac catcgccgag
    16561 cgctaccatc ccggcaccca ccagtggtcg ggcccccaca gccgcgccta cagcgaccgg
    16621 ctcgacgccg gaatctccgg cttcctgtct gaacggacgg gcgtcgaagt ccgcccccat
    16681 ccaaaggcgg gcctgagaga caggttcgaa ggcaccgcgc cgctgccctg cccgtccgga
    16741 cttgtggacc gtttccggtc gccgccgcat ggggagacgg aactgcgaag cacgttcatc
    16801 cgccgtgcgg gagaaggcga ctccatccag ggcacgacct ggatgtccga ggatgcctgc
    16861 ctgggatcgg tcagtcgcga caacctgtgg gaccagcgcc acgcgcgtct cgggtactgg
    16921 cggacggagg aggatcccgc agtagtgctg cggctgcgat tcctgcacga cgggcgcgac
    16981 ttctcatccg cctgcgcgcg caacgcccag agcggcccgc gcatcctctc cgcgatcggc
    17041 ttgctaaccg gaatgggcga cgcccaccat caactcgacc gcacggccga cggcgcattc
    17101 gacgctgagg atttccgcgt ccgctatgag ctggtcggag agggcgtcgc gggaagggct
    17161 ctcgaaggca gccgcttcga gctctccgca ggctcctgtc aggcgatagt ccacaccgcc
    17221 cccggccggt tcggtccgca cgagctggtc tgggagatgg agcgcgccga agggcgagtg
    17281 tgccttgacg gggtgtgcta ccgcgggccg cgccggaggt tcgagccggt ggagctcggc
    17341 gacgtggtga tcttcgccgg gctggagttg ctccgggccg accagcctcc atcggcgtcg
    17401 ggccctgcag cgtgcgaacc agatggaggg atggtggatg tttcgtggcc cgtcgcggga
    17461 gggcttagga tcctggcacc cacacgcgca caaccttacc catgagagag gacagagctt
    17521 gcccggaaga ttccgccgcc acgaccggcg ccccgtcacg caactgaacg gaatctggga
    17581 cttcgcgttc cttggcgacg aggaccccga cgcggtggac gcggcctcca tcgggttcga
    17641 cgacgtgatg gccgtgcccg gctgcttcga cgccacgccc cgctacgcgg gcaggagggg
    17701 gctggcggcc taccgcaccc gcgcgttcct ccccgagcat gggcgatacc gactgatcct
    17761 tgacggcgtg caccactggt gccgcgtctt cgtgaacggc gagaagatgc gcgaccacgt
    17821 cggtggcttc acccgcttct gtgtggactt cacgggccgc gtggacgaag aggtcgagat
    17881 cgtcgtgctc gtggacaatc gcttcgatta cgaccggtgc ccgctccacc tcgagtactt
    17941 cgactggtac cactttggcg gtatcgcgcg cccggtcgaa ctacatcgac tggctgaccc
    18001 ctggatcgag ggcctcacca taatcacaga ggccgtcgaa tcgcgcaggg tagccgtcac
    18061 catcgactac gacgccaccg cccctccggc aaagaccgac cttaccatca cctgcgacgg
    18121 ccgggtcgtc ctggacgaga ccgtggactt gggccagacg ccgggccgga tagagcgcgt
    18181 gctggagttg ccagggacaa gactctggtc gcccgatgag ccaaacctgc atgtgatcca
    18241 ggctacgctg ggcgaggacg acgccagaga acggataggc atacgacaga tcagtgtaga
    18301 agggcgtagc ctgctcgtaa acggcgagcc cgttcgtctg cttggcttca acaggcacga
    18361 ggcgcaccca cagttcggcc acttccagcc ggaggccctg ctcgtctcgg acgttcaaca
    18421 gctgcgggat gcaggctgca acttcgtacg gggcagccac tatccccagg acgttcgctt
    18481 cctggacctg tgcgacgagg ccggcatctg cgtctggcaa gaggccatcg gctggcaaca
    18541 tactacagag cacctgactg acgaaacctt cgtccgggcc caactgacca acatcgagga
    18601 gatggtagcc gcctccgcca accggccctc cgtgatcctg tggggcatcc taaacgagag
    18661 cgagagtcac gatcccgcgt gccgtctggc ttacgagcgt ttacttggtc gactgcgaga
    18721 gctcgaccct acgaggcccg tcacctacgc cagcaaccac cctttcgacg atgcgtgcct
    18781 ggacctggcg gacgtcgtct cgatcaacag ctatcccggc tggtacttcg gcggccttga
    18841 ggatgtaccg gatgagcttg acagaatctc cgagcatctg gactccgtcg gccaggggga
    18901 cgaaccgctg atcatctccg agatcggcgc cggtgcgatc cccggctggc gcgatcctca
    18961 cgcggcccgc tggacggagc gctaccaggc cgagttgctc gacacggtca tccgccacct
    19021 gttcgtggac agggaccggg cctgtggcct cgccatctgg ctgtacaacg actttcgtac
    19081 cacggaagag atcggtaggg cgcttggccg tcccaggggc ttcaacgaca agggcgtcgt
    19141 tgacgagtac cggcgtccca agctcgcata cgagacggtc aggcgccgct tcctgacgct
    19201 caccgatgac ggagactaga gagaccatgc tcggcaggca cgatcccgct tccatcgaac
    19261 gcgcactgaa cgaggcgtca ccatcgccgc ccttcccacc ggcgtcggac cgggcagcgt
    19321 gggatgaggt gcgcgaagtc atcggggagg aacgggtatc ggagctcttc gacagagcca
    19381 gcgccaacac agacgtcccc gctctcccgg ccacgctcta cctggagttc gcgcgcaacg
    19441 gtagccggga ggggtacgag cggcccgcga accgcaggcg ggagatgctg tcggacctgg
    19501 ctctggccga gtgtctggaa ggagaagggc gcttcctgga ccctctactc gacgtggcgt
    19561 gggcgatctg cgaggagagc agctggtcgt ggcccgcgca ccagggagac ctggccgtta
    19621 tggaccaccc ggtgatcgac ctgagcgccg ccatgaccgc gctgtacctg gctgaactcg
    19681 acctgctcct gggagaccgc ctcgaccccg tgctgggacg gcgcatcagg cacgaggtag
    19741 accgccggtg tctggctcct tacatgagac gccacgacca ctggtggctc ttcgactccc
    19801 ctgagaggcc ggtaaacaac tggacggcgg tgtgcaacgc gggggtggcg ggggcggcta
    19861 tccacctgga aaccgacgcg gcccggctgg cggagatcct ggcccgcgcc gcccgctccc
    19921 tggacgacta cctctcaacg ttcgacgagg acggcggctc ctcggagggg ccggcctact
    19981 ggtcttacgg gttcggttac ttcaccgtcc tcgcccacct ggtgggacac cgcaccggct
    20041 ggaaagtgga catcttcgag ggcgagcacg tgcgcagggt cgcacggttc ccttcgcgca
    20101 cccggctcgg cccgggcctc tatgtaaact tctcggactg cgaccggcac atctccttca
    20161 tccccgccca cctgtcttat ctttcgcggt ggttggggtt gcctgatctc gcgcgtctcg
    20221 cccgcgagca aaccggcgat gtcgaacgcg aacgtgagtt gacctggagt ctacgcaacc
    20281 tcttctggcg tcctgaaccc gtcgaggggc attttgtccc cgcgcgccac gactggttcg
    20341 gcggcatgat gtggatgatc tcccgccagg accccgatga tcccgacgcc ctggtactcg
    20401 cgaccaaggg cggccacaac ggtgagatgc acaaccagaa cgacgtgggc aacgttatag
    20461 ttcatgttgg cgctgagtcc gtaatcgccg acatcggacg cggacgttac acgaaggcct
    20521 acttcggccc agagcgatac gagcacatcg ccaactcttc ccaggggcac tccgttcccg
    20581 tccccaacgg gcaagagcaa cttcctggcg gcgaacacgg cgccagactg ctgcaacacc
    20641 gggctgatga tcccgccgac ctggcggcgt tcgagctacg ggacgcctac ccgactgagg
    20701 ccgacctcga ctcgctcaag cgcacggctg tcctgcatcg cgggccgccc gcctgggtag
    20761 agttgacgga cgaggtgagg ttcacgtcgg agccaggtag cttcgagagc cccctgatca
    20821 ccttcgggga agcggaggtc tcccccgact cggtgctgct acgcggagag cgtggcaccc
    20881 tgcgcgtcta cttcgacccc gaatcagtgg tcccgcgcgt cgaggttgtc gagggcgtgg
    20941 acctggccga gggaccagcc gacgtgcgcc gcgtggtatt cgcgctccag gaacgagtgc
    21001 aggaggggtc cgtccatctg cgcatcgaac cggagaagtc atcgtcaggc tcggaccctg
    21061 gatagacgtc atcgcggagg cccgtatgcc ggctcagtgg atcgcccgga tacggtaaga
    21121 gatcccctcg ctcttatgcc gaaactctat cctcagcagg ccgcagagcc cgcgcctgct
    21181 ctgaaagccg ccggtcaaat ggaccacgcg cttcacggga gcgtcgtagg agatctcgac
    21241 ctgttcccgg ccgtggatca gacacacccc tccggcctga accctcgcct ccgtgaaggt
    21301 tcctgtgcca gggtcccgca ggtacgggac tagcagcgcc ttgggttctt cgagccccgg
    21361 cgtagcctcc tcgacgtaga ccccatccgt ctccacgcgc gcagcgaggc ggtaccggta
    21421 cgggcttcca ctgccccgcg cttcgagcct caggccggcg ggcgtccagt ccgtctcgat
    21481 acgcccgatc agcatcaggt ctccctgcga cgcgggtcca gtacttccgg ccatgccgag
    21541 gttgatgaac tttccgcttt cgaggtgctt cgcgaccgag gcgaagtgca ggaaccgccc
    21601 tgggccgaag gtgagatcga cgagcccgaa ggcgtcgtaa tccggggcag ggcgggcgtt
    21661 gacgtgcagg ctgtaggcgc cattgtgcaa gaccgctctg taggtggggt cgtgctctag
    21721 ccagacggaa gactctcgct cgccggacgg ctgcgtaacg gggagatcca acccgttcga
    21781 caccgcaatc gcgaggaatg ccgccgccag gttagcgtag tgaccatcgg ccgtgtaacg
    21841 ctcgtagccg accctgtacc ccggtggtag aaggttctcg accgggctat acggcccgtc
    21901 ccgccgctgc cagcgccgga acgacgaaag ggcgaggtag gccgcttcgt cggcgtgtct
    21961 cgcaagctcc gggtcctttc cgcgaaagtg gttggcaacg tgggtaaagt acgcgcactg
    22021 ggccccgaga gtccacgtct gcccggtgct gcgatgggcg gaggcaaggg atccgtcgga
    22081 gagctgcaca ccgaggcttc ggcgcaggcc cgtgcggatg aggcgctcca ggtcttcgcg
    22141 ccgccggccg tcgtagcctt ccatcagcat gtcggccaga tggtagcggg tgaagagatc
    22201 gtaggagttc gggtgtccgg gttcctggta gagtccctgc tcggtcagga tgtgcgcgtc
    22261 gaagaacgcg tctagccaga gatctatatc ttccttcgag aacggagaga ggccttcacg
    22321 aagacggagc cactcgcccg agagccagac ggaggcccag ttcgagggag gtcgtttggc
    22381 gaaagcgcgc tcgatgtcca ccccggagat ccgtcgtgca aagacttcgg tgccgtccac
    22441 cctcatgctc ctgaggatgc gaaacgtctt gatgatcggc ggccagaaga agtccctgtg
    22501 gttgctgcgg gtcaccgcgc ccgtcgccgg gtcgaagccc gaagcggttg gagggaggcc
    22561 ggggtccgag acatggtcaa gggctacctt cagaccgcga acggcgcggt caacgtaggc
    22621 cgaacgtctc tcaccaccgt cccccatggc gagcacggcg ttgcagagag cgtgataagg
    22681 agtcccgtac tgcgtcggtt caccgaagac cggatcgtgc aattctccgg aaggctgcag
    22741 gtggggtgcg taactctcga cggcgtccct caagatctcg accggatgga ctgcttccgc
    22801 ggcatccggc gtatcgagac tccgcccgct tcccttatca acatctttcc gcacgttcct
    22861 accttttcgc ggccaggcgc gtcgggtgaa tggcgcctcc gatccactcg ccttccgccg
    22921 gaaggcggat caagccttct cctcctgaac accttttgta aaggg
//
LOCUS       DATAIR010000194         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_12408, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000194 DATAIR010000000
VERSION     DATAIR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12408"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VHM69_04720"
     CDS             <1..634
                     /locus_tag="VHM69_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX2739728.1"
                     /translation="VTPLAGQGKVRLRITTRAATEREAQEKIEPVEKEVIARLSDYYF
                     GEDDETLEGAVGRLLKEREATLALAESCTGGLLAKRLTDMPGSSAYFIEGLVTYSNES
                     KERLLGVSHELIIEHGAVSEPVARAMAEGARTTSGADYGLSVTGVAGPDGGTEDKPVG
                     LVFVGISDQKGTFAEKLDLSAWARSRDSIRERSANRAFDLLRLRLDEHKS"
     gene            complement(913..>1649)
                     /locus_tag="VHM69_04725"
     CDS             complement(913..>1649)
                     /locus_tag="VHM69_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2739729.1"
                     /translation="WRILEGKEPVAPDPELGHAANYLYMLSGEVPGSEYTRALETYFN
                     TVTDHGMNASTFAARVIVATRSDFVSAVVGAIGALKGPLHGGAPGPALDVVFEIGESS
                     RAEAYLREKLGRGERLMGFGHRIYRVRDPRADVLAAAAKRLYTTDGDRELYELALEVE
                     QTALWLLAEHKPGRNLQTNVEFYTALLLHGLGLPAQLFTPTFAAGRVAGWTAHCFEQR
                     ALDRLIRPQSAYVGERDRRWVPLEER"
BASE COUNT          320 a          522 c          510 g          297 t
ORIGIN      
        1 ggtcaccccg cttgccggcc agggcaaggt tcgtctgcgc ataacgaccc gcgccgccac
       61 cgagagggag gcgcaggaga agatagagcc tgtagagaag gaagtcatcg ctcgcctcag
      121 cgactactac ttcggcgaag acgacgagac cctcgaaggc gccgtcggac gcctcctcaa
      181 agagcgggaa gcgacccttg ccctcgcgga gagctgcaca ggcggactcc tcgccaagcg
      241 cctgacggac atgccgggat cctcggctta cttcatagaa ggactcgtaa cctactcgaa
      301 cgagtccaaa gagcgtctgc tcggggtatc tcacgagctg atcattgagc acggcgcagt
      361 ctccgagccc gtggcacgcg cgatggctga gggcgcaagg acgacttcag gcgctgacta
      421 cgggctctcc gtcacaggcg tcgccggtcc agacggcggg acggaggata agcccgtcgg
      481 cctcgtcttc gtcggcattt ctgatcagaa gggcacgttc gcggagaagc ttgacctcag
      541 tgcatgggcg agatccaggg actccataag ggaaaggagc gcgaacagag ccttcgacct
      601 cctcaggctt cgcctggacg aacacaaatc ttgacgatca cccttgctgg atacaaaacg
      661 tggtagtaaa cttcgcgaag ttgacggcac gcgaaaggag gtgatccaga tagacgcatc
      721 gagtagtagt cgcgggtatc ttctgggaga ggtggctctg acctaggccc accaaaatac
      781 ccggcgggat cctgggttac tttatccgcc tcccatgtag tgaaggcgga cgtgctgaaa
      841 gacgggatac ctgcgtaaca ctcagaaggg tacggccccc ggatttctcc gggggtcgtt
      901 tttttgtctc atctacctct cttcgagcgg aacccagcgc ctgtctctct cccctacgta
      961 agctgactgc ggcctgatga gacggtctaa tgctctctgc tcgaagcagt gcgccgtcca
     1021 gccagcgacc ctgcctgcgg cgaacgtcgg ggtgaagagc tgcgcgggca acccaagtcc
     1081 gtgcagcagg agcgccgtgt agaactcgac gttggtctgc aggttccggc caggtttgtg
     1141 ctccgcgagc aaccatagcg ccgtctgttc tacttccagg gccagttcgt acagctccct
     1201 gtccccatcg gtcgtgtaca gacgctttgc ggcggcggcg agtacgtccg ccctcgggtc
     1261 gcgtacacgg tagatcctgt gcccgaaccc catgagacgc tcgccgcgcc cgagcttctc
     1321 cctcagatac gcctcggccc tgctcgactc cccgatctcg aacaccacgt cgagcgcagg
     1381 ccccggcgca cccccgtgta gcggtccctt gagcgccccg atggccccga ccacggcaga
     1441 gacgaagtcc gagcgggtgg cgacgatgac cctggccgcg aaggtggagg cgttcatgcc
     1501 gtggtcggtc acggtgttga agtaagtctc cagcgcccgc gtgtattccg agccaggcac
     1561 ctcgcccgaa agcatgtaaa ggtagttggc cgcatggccg agctccgggt cgggggccac
     1621 tggctccttt ccttcgagaa tccgccagt
//
LOCUS       DATAIR010000195         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_12471, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000195 DATAIR010000000
VERSION     DATAIR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12471"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VHM69_04730"
     CDS             complement(<1..695)
                     /locus_tag="VHM69_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739730.1"
                     /translation="MEDFLGSIGPILQSTVRNATPLVFAALGGLFSERSGVVNIGLEG
                     LMLISAFAGVVGAHFSGSALVGLGTALAAGLLFALIHALMCVTFEADQIISGTAINLL
                     ALGGTGFLMVVIFGAGGTSPRVNGFASVAIPLLSDIPIIGRALFDQSLLVYLMYVLVP
                     VVFFVVFRTPFGLRLRATGEVPEAVDTAGVNVARMRYYGVALSGLLAACGGVYLSMGL
                     LSAFTENMTGGRG"
     gene            complement(695..1816)
                     /locus_tag="VHM69_04735"
     CDS             complement(695..1816)
                     /locus_tag="VHM69_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739731.1"
                     /translation="MSEGPGVTEGRGSALGRMVAGAAGALLFPLIAIVLSFIIGAVIV
                     LATGNNPIAAYAALLEGAVGDPFALGRTLLYTTPLIFTGLAVAVAFRAGLFNIGGEGQ
                     LFIGAVTAAWLGVSLGFLGPVAIPLVLAASAITGFLWGSIPGVLKAYFGAHEVITTIM
                     LNYVGIYFAYYLAQHPLGQKGPIPGTETIDLAARVPTISAALGRANFGFFIAIAAAVV
                     VYLLLWRTRRGFELRAVGLSPGAANYAGMRLGLNTVLALAIGGSLAGLGGGVEVLGVY
                     GNMDVPFVTNLGFNGIGVALLGRNHPVGVVLGAILFGALTSGAQEMQFATDVPLQLAN
                     VLLAVILLLVTATKLVELIVGKRARDFAAGTHLENERGL"
     gene            complement(1813..3375)
                     /locus_tag="VHM69_04740"
     CDS             complement(1813..3375)
                     /locus_tag="VHM69_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739732.1"
                     /translation="MAEESGGVVGAADAPVVLEMRGVTKRFGPVTANDDVSLTLHRGE
                     VLGILGENGAGKSTLMKILYGLYTPDEGEIFVDGERVEIRDPKDAVTRGVGMVHQHFT
                     LIPPLTVAENIVLGAEPRKGLSLNLEEAVRATEELSERYGLRVDPRARVADLSVGVRQ
                     RVEILKALYRNVRILVLDEPTAVLTPQETEDLFAVLKELVADGLSIILITHKLGELLG
                     VGDEITIIRDGRVVGTVKAAETNERELARLMVGREVLLRVEKEEAQTSDEVRLSAENL
                     TVRSDTGEEAVAGVSLEVRGGEILGVAGVDGNGQTELAEALAGTRAIEGGAVYLDGED
                     VTRLGADALQERGLAYVPEDRASKGLVQDFALYENNALKTYDAPPFSRFGWIFPKVMR
                     RRAAESLRAYDVRPADPDARAGSLSGGNQQKAVLARELSGDPGVVIAAQPTRGVDVGA
                     IEFIHRQLLKQRSEGKAILLISLELEEIRSLSDRILVIYAGRIVGEVGPDATDEELGL
                     LMAGHDIEEKSA"
     gene            complement(3391..4428)
                     /locus_tag="VHM69_04745"
     CDS             complement(3391..4428)
                     /locus_tag="VHM69_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2739733.1"
                     /translation="MRTRILVITLALALSLAAVGCGGSGGGGEGGAEVRPGLVLDVGG
                     LGDDGFNDSAYAGFKRAEKDLGVKGDFLESTAPTDYADNLTQLAENGFDPVIAVGYLM
                     TEDITEVSEQFPDTQFAIVDSVVDTPNTMDLVFREQEGSYLAGIVAGLMTQEDTEYTT
                     SDEKVVGFLGGQTGPLIEKFQAGYEAGVKSVCPDCTVLVKYAGATPDAYNEPAKGKEI
                     SLQQISQGADVIYHAAGNTGAGLFDAASQEKIFAIGVDLDQAKLFPKDPILTSVVKRV
                     DNAVYQTIDSVSKGEKPKGTTVDRGLKEKGISLAPFGRFDSMVPQEVKDEVDKAEKGI
                     ISGDIEVPTKP"
BASE COUNT          799 a         1505 c         1458 g          769 t
ORIGIN      
        1 aagcctcggc ctccggtcat gttctcggtg aaggcggaga gcaggcccat cgagaggtag
       61 acgccgccgc aggctgccag caggccggag agggcgacgc cgtagtagcg catccttgcc
      121 acgttgacgc cggccgtgtc caccgcctcg gggacctcgc ccgtggccct gagcctcagt
      181 ccgaaggggg tgcggaagac cacgaagaag accacgggga cgagcacgta catcaggtag
      241 acgagcaggc tctggtcgaa gagagcgcgg cctattatgg ggatgtccga aagaagtggg
      301 atcgcgacgg acgcgaagcc gttcacgcgc ggtgatgttc ctcccgcgcc gaagatcaca
      361 accatcagga agccagtacc gcccagtgcc agcaggttga tcgcggtgcc cgagatgatc
      421 tggtcggcct cgaatgtcac gcacatgagc gcgtgtatga gggcgaagag cagtcccgcc
      481 gcgagagccg taccaagtcc cacgagcgcg ctcccgctga agtgcgcccc tacgaccccc
      541 gcgaaggctg agatgagcat cagcccttcg aggcctatgt tgacgacccc gctcctctcg
      601 ctgaacagac cgccgagggc ggcgaagacg agcggcgtcg cgttcctgac ggtggactgg
      661 aggataggac ctatggagcc gagaaagtct tccactacaa gccccgttca ttctcgaggt
      721 gggtgcctgc ggcgaagtcg cgggcgcgct tgccgacgat cagctcgacc agcttcgtgg
      781 ccgttacgag cagcaggatg acggcgagca gcacgttggc aagctgaagc ggcacgtccg
      841 tggcgaactg catctcctgt gcgcccgagg tgagcgcacc gaataggata gcgccgagga
      901 ccaccccgac agggtggttg cgtccgagga gtgcgacccc gatgccgttg aagccgaggt
      961 tggtgacgaa gggaacgtcc atgttgccgt agaccccgag cacctcgacg ccgcctccga
     1021 ggccggcaag agagccgccg atggcgaggg ccaggaccgt gttcagtccg agccgcatac
     1081 cggcgtagtt cgcagccccc ggcgagaggc cgaccgcgcg tagctcgaat ccgcggcgtg
     1141 tcctccatag caggaggtag accacgacgg cggcggcgat cgcgataaag aaaccgaagt
     1201 tcgcccgccc gagggccgcg ctgatcgtcg gtacgcgggc ggccaaatct atcgtctccg
     1261 taccagggat cggccccttc tggcccaaag gatgctgggc caggtagtac gcgaagtaga
     1321 taccgacgta gttgagcatg atcgtcgtga tcacttcgtg cgccccgaag taggccttga
     1381 gcacaccggg gatggatccc cacaggaagc cggttatcgc gcttgccgcc aggacgaggg
     1441 ggatggcgac cggcccgaga aagccgagcg agaccccgag ccaggccgcg gtgacagccc
     1501 ctatgaagag ctgtccctcg cccccgatgt tgaagagccc ggcccggaac gccacggcga
     1561 cggccagccc ggtgaagatc aggggcgtcg tgtacagcag ggtgcgtccc agcgcgaagg
     1621 gatctccgac cgcgccctca aggagggccg cgtaggctgc gatagggtta ttgcccgtcg
     1681 cgaggactat gaccgccccg atgataaaag agaggacgat ggctatgagg gggaagagca
     1741 gcgcccccgc ggccccggcg accatccgcc cgagggccga gcctctcccc tcagtcacgc
     1801 cgggcccctc gctcacgcgc tcttctcctc gatgtcatgc cccgccatca ggaggccgag
     1861 ctcttcgtcc gtcgcgtccg gcccgacctc gccgacgatc ctgcctgcgt agatcacgag
     1921 tatgcggtca gagagggagc ggatctcttc gagctccagg gagatcagca atattgcctt
     1981 gccctcggag cgttgcttca ggagttggcg gtgtatgaac tctatcgcgc cgacgtcgac
     2041 gccgcgggtc ggctgggccg ctatcacgac gccgggatcg cccgagagct cgcgcgcgag
     2101 gacggccttc tgctggttac cccctgagag agatcctgcc ctggcgtcag ggtcggcagg
     2161 acgcacgtcg taggcgcgca gcgactccgc cgcccggcga cgcatgacct tcgggaagat
     2221 ccaaccgaac cgcgagaaag gcggcgcgtc gtaggtcttc aaggcgttgt tctcgtagag
     2281 ggcgaagtcc tgcaccagtc ccttggacgc cctgtcctcg ggcacgtagg cgagcccgcg
     2341 ctcctggagg gcgtcagcgc cgagccgggt aacgtcttcg ccgtcgaggt agacagcgcc
     2401 gccctcgatg gctcgggtgc cggccagagc ctccgcaagc tccgtctggc cgttcccgtc
     2461 aacgccggcc acgccaagga tctcaccgcc gcgtacttcg aggctcacgc ctgccacagc
     2521 ctcctcaccc gtgtcggagc ggacggtcag gttctccgcc gacaggcgca cctcatcgga
     2581 agtctgggcc tcctccttct ccacgcgcag gagcacctcg cgcccaacca tcaggcgcgc
     2641 cagctccctc tcgttcgtct cggcggcctt caccgtgccc accaccctgc cgtcgcggat
     2701 gatcgtgatc tcgtcgccga cgccgaggag ctcccccagc ttgtgggtta tcaggatgat
     2761 ggagagtccg tcggcgacca gttccttcaa gacggcgaag aggtcctcgg tttcctgggg
     2821 tgtcaggacg gctgtgggtt cgtcgaggac aaggatgcgc acgtttcggt agagggcctt
     2881 caagatctcg accctctggc gcacccccac ggagaggtcg gccacacgcg cccgcgggtc
     2941 taccctgagg ccgtagcgct ccgagagttc ctccgtcgcc ctcacagcct cctccaggtt
     3001 cagggagagg cccttgcggg gctccgcgcc gaggacgatg ttctcggcga ccgtgagcgg
     3061 cgggatcagg gtgaagtgct ggtgtaccat cccgacgccg cgagtgacgg cgtccttggg
     3121 gtccctgatc tccaccctct cgccgtcgac gaagatctca ccctcgtcag gggtgtagag
     3181 gccgtacagg atcttcatca gggtagactt gccagcgccg ttctcgccga ggatgccgag
     3241 gacctcaccg cggtgcagcg tcagggagac gtcgtcgtta gccgtgacgg ggccgaacct
     3301 ctttgtaaca ccccgcatct ccagtacgac aggggcgtct gccgccccga ccaccccgcc
     3361 actctcctcg gccaagccta tctcccgcta ttaaggcttc gtggggactt cgatgtcgcc
     3421 gctgatgatc cctttctcgg ccttgtcgac ctcgtccttg acttcctggg ggaccatgct
     3481 gtcgaagcgc ccgaacgggg cgagggagat ccccttctcc ttcaggccgc ggtccacggt
     3541 cgtgcctttg ggcttctcac cctttgagac gctgtctatc gtctggtaga cggcgttgtc
     3601 cacccgcttg accacggagg taaggatggg gtctttgggg aacagcttgg cctggtcgag
     3661 gtcgacgccg atagcgaaga tcttctcctg gctcgccgcg tcgaagaggc ccgcgccagt
     3721 attacccgca gcatggtaga taacgtcggc gccctggctg atctgctgga ggctgatctc
     3781 ctttcccttc gccggttcgt tgtacgcatc cggcgtcgcg cccgcgtact tgacgagcac
     3841 cgtgcaatcg gggcacacgg acttcacgcc agcctcgtag ccggcctgga acttctcgat
     3901 caatggcccc gtctggccgc cgaggaagcc gacgactttc tcgtcggagg tggtgtactc
     3961 ggtatcttcc tgggtcatga gcccagcgac tatcccggcc aggtagctcc cctcctgctc
     4021 cctgaagacc aggtccatag tgttcggcgt atccacgacg gagtcgacga tggcgaactg
     4081 ggtatcgggg aactgctcgg agacctcggt aatgtcctcg gtcatgaggt agccgacggc
     4141 gatcaccggg tcgaacccgt tctcggcgag ctgtgtgagg ttgtcggcgt agtccgtcgg
     4201 ggcggtcgac tccaggaagt cacccttgac cccgaggtcc ttctcggccc gtttgaaccc
     4261 cgcgtaggca gagtcgttga agccatcgtc acccagacca cctacgtcga gcaccagccc
     4321 gggacgaact tcggcaccgc cctcaccccc gccgcctgag ccgccgcagc ctaccgctgc
     4381 aagagacagc gccagggcta gagtaatcac aagtattcgc gtgcgcacag aacctcctca
     4441 ttcgccgagc ccgagcctct ccgggcggct tgacgcttcg actgactaat cggtaaccgc
     4501 atcatataga gtaataaaga ccggcgcaaa g
//
LOCUS       DATAIR010000196         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_427594, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000196 DATAIR010000000
VERSION     DATAIR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427594"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2114
                     /locus_tag="VHM69_04750"
     CDS             <1..2114
                     /locus_tag="VHM69_04750"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2739734.1"
                     /translation="LEETSDFLGRTMDLSLTAERVATLERGTEGWIVGLQLAAHALRG
                     GDARPVEAFTGSARHVVDYLAEEVLSRQPEDVREFLLKTCIVETLSGPLCEGLTDTTD
                     GQEMLERLERANLFLVPLDEGGRYYRYHHLFAAFLRERLRRMHPDAVPELHRRASLWY
                     EGDGCLSAAIEHALDAEDFDRAAGLIEEETGVRRRYVDASLLLRWLGTLPDWLVRQQP
                     QLCLLYAWALAHSGELEDAELRLRDTEAALKLEDGVSTDALTDEERTMLGEICIVRAR
                     IAAMRENAPLTTELSNRALELLPRGEQHLRGDVSLDLGHAYCSIGDLESASEAFAMAA
                     AIGRDVDDLRTALFALRYQASLEISRGRLREAEALLQDGRRLAESRPEGVPSVAGIIH
                     TGMGELLYERGALDEAQRLLETGIEHGRRSGEAKILVYGYVNLARVLMARGDAEEAHS
                     LIREAGRLTPKWPLIWAWQARFYLAQGDVASAARWAREYGATRDYLSYPRHFERITMA
                     RVLLGEGRTDEALSSLGQLHEDAVSDGRMAHVIELLVLLALASERQGAREVALGHLDL
                     ALALAEPEGFVRVFVDEGPPMAALLERLLRESRHQGSYATTDGYAGRLLERFATGLYD
                     NPGNGAPRSGHVPGLEPLSQREIEVLELVAAGLSNAQIARELYLSVGTVKAHVHHIFG
                     KLIVRNRSQAVARARELRLLT"
BASE COUNT          403 a          704 c          732 g          362 t
ORIGIN      
        1 ctctcgaaga gacgtcagat tttctcggcc gtacgatgga ccttagcctc accgcagaga
       61 gagtcgcaac gttggaaaga gggacggagg gatggatcgt cggcctgcag ttggcggcgc
      121 acgccctccg ggggggcgac gccagacccg tcgaggcttt cacgggaagt gcccgccacg
      181 ttgtcgatta cctggccgag gaggttctct cccgccagcc ggaggacgtg cgggagtttc
      241 ttctcaagac ctgcatcgtc gagactttga gcggtccact atgtgagggt ctcactgaca
      301 ccaccgacgg acaggagatg ctggaaaggc tggagagggc caacctcttc ctggtgccgc
      361 tggacgaagg gggacgctac taccgttacc atcacctttt cgccgccttc ctgcgcgaac
      421 gcctgcgacg catgcacccc gacgcagttc ccgaactgca tcgccgcgcc agcctctggt
      481 acgagggcga cggatgcctc tccgcggcga tcgagcacgc cctggacgcg gaggacttcg
      541 accgggcggc gggcttgatc gaggaggaga caggcgtgcg gcgcaggtac gtagacgcct
      601 ctttgcttct ccgctggctg gggacgttgc cagattggct ggtgcgccaa cagccgcagt
      661 tgtgcctgct ctacgcctgg gcgctcgcgc attctggaga gctggaagac gccgaactcc
      721 ggctccgaga caccgaagcc gccttgaagc tcgaagacgg agtttcgacg gatgccctca
      781 ctgacgaaga gcgcacgatg ttaggcgaga tctgcatcgt gcgcgcccgc atagcggcca
      841 tgcgggagaa cgcgccgctt acgacggaat tatccaatcg ggccctcgaa ctgctgccgc
      901 ggggcgagca gcatctgcgc ggcgacgtct cgttggacct cggacacgcc tactgctcga
      961 tcggggatct cgagtctgcc agcgaagcgt tcgccatggc cgcagccatc ggcagggacg
     1021 tcgacgacct tcggacggcc ctgttcgccc tcagatacca ggcctcactg gagatctcca
     1081 ggggacggct cagggaggcc gaagccctgc tgcaggacgg acggcgactt gccgaaagta
     1141 gaccggaggg ggtgccgtcg gtagccggca tcatccacac agggatgggc gagttgctct
     1201 acgagcgcgg agcactggac gaagcccaac ggctcctgga gacgggtatc gagcacggca
     1261 ggcgcagcgg cgaggcgaag atactggtct acggctacgt caacctcgcc cgcgtgctga
     1321 tggcccgcgg agacgccgaa gaggcgcact ccctcatccg cgaggccggc aggctcacgc
     1381 cgaagtggcc gctaatatgg gcgtggcagg cgcgtttcta tctggcgcaa ggcgacgtgg
     1441 cgtcggcggc gcgttgggca cgagagtacg gggcgacccg ggattacctg agctacccac
     1501 gccacttcga gcggatcacg atggcccgcg tgttgctcgg ggagggcagg acagacgagg
     1561 cgctctcctc cctcggccag cttcacgagg acgccgtatc cgacggacgg atggcgcacg
     1621 tgatcgagct gctcgtgctg ctcgctctcg cctccgagcg acagggtgcg agagaagtgg
     1681 cgttgggcca cctcgatctc gcgctcgctc tggccgaacc ggaaggcttc gtgcgcgtgt
     1741 tcgtggacga gggaccgccg atggccgcat tactcgagag gctactccgg gagtcgcggc
     1801 accaaggatc ctacgccacc accgacggct acgcgggtag gctgttggag cgctttgcca
     1861 cgggccttta cgacaaccca ggcaacgggg caccacgaag tggccatgtc cccgggctcg
     1921 aacccttgag ccagcgcgag atcgaggtgc tcgagttagt cgccgccggc ctgtcgaacg
     1981 cccagatcgc cagggaactc tacctctccg tggggaccgt caaggcgcac gttcaccaca
     2041 tcttcggcaa gctcatagtg cgtaaccgct cccaggccgt cgcgagggcg cgagagttgc
     2101 gcttgctcac ctagcctctc caaattaaac cggcagtaaa ccaccagata caaccttcgc
     2161 tagattaagc ccgggacccc ggctcctaga ctggcctcat t
//
LOCUS       DATAIR010000197         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_12487, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000197 DATAIR010000000
VERSION     DATAIR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12487"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VHM69_04755"
     CDS             complement(<1..758)
                     /locus_tag="VHM69_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2739735.1"
                     /translation="MNESMNEPAGKRTGIGEAVTSALRQPSFLRVMLVLAATVVVLVG
                     IRLSAPILNPILFAIVLSLLFSPVYAWLKNRGLPSPLALVIMLILVGAIFAVLFFILG
                     VSISRFSERIGYYTTQLNGQLDNIDALIKRSGLSNVDVRDVVKPSALADALGTVLAGI
                     AGFLSDLFLILMIMLFLLGEGPAMLDRLRAGSGRDNPQVQRLTTVGRSVVRQFGLRAV
                     LNLFTAAGITVLLFLLGVDFPLMWGILAFFLSFVP"
     gene            complement(842..1942)
                     /locus_tag="VHM69_04760"
     CDS             complement(842..1942)
                     /locus_tag="VHM69_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2739736.1"
                     /translation="MARTVAIALLTAVVVLGALFLLWQVHTFIGWFVISLFLAAVLNP
                     AVNWLQRRHRLMKRPLAIGLTYLGVLVALLLVVGIFVPVLVDQINGFIKFVTTAANAP
                     EGPTEYIKGLAKENGLGGLFERFSDQIAQLRQQLGDLLRNLFSASGQVAVGLAGFVAA
                     LATVLTLTFFLLLGSERYVNAGVGLFPEAHRPLVRRLLTQSAGAISGYITGNLAISVI
                     CGITTFIVLLVLGMPYAAPLALLVAVLDLVPLVGATLGGALLVVVGLFVEPWKAVVLL
                     IFVLVYQQVESNFLQPIVYSKAVQLNGLVILIALLVGGQLLGIPGALLAIPVAEIIRI
                     VVTELLAYRRTRQEAEEPAVASPPPAPSEVAE"
     gene            complement(2209..3489)
                     /locus_tag="VHM69_04765"
     CDS             complement(2209..3489)
                     /locus_tag="VHM69_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2739737.1"
                     /translation="MVDLVVLALVTFAFSLVSRRLEGTILTAPIVFVVAGAILGPGGL
                     GLVEIRLDDHTVLLVGEIALAIVLFTAAASINLSSLRRNEGLPLRLLCVGMPLTIALG
                     AAAAVLLLTDIAFWEAAIVGTVLAPTDAALGQSVVSNPRVPIRVRQALDVEAGLNDGL
                     SVPFLALFLTLAVAEEELQPASYWLLFAVEQIGLGTLVGVGVGLVGGWLVSLASKREW
                     MTDSFQRLALLALAIVAWALADQIGGNGFIAAFVGGLVVGPTVERVGERLLRFTEAEG
                     QLLNLSVFFIFGVLAIGVIQPLSWEVAVYALISLTLVRMLPVAVSVVGTRLSGISTLF
                     IGWFGPRGLASIVLGLIVVAEAPELAGREEMEAVVALTVLLSVLLHGVTAAPLAEAFA
                     RRVDEMSLDAPEKGERIEVHTREDTLPSSDPKGR"
BASE COUNT          797 a         1136 c         1123 g          611 t
ORIGIN      
        1 ggcacgaagc tcaagaagaa ggccaggatg ccccacatca gggggaagtc gaccccaagc
       61 aggaacagca agacggtgat gccggcggcc gtgaacaggt tgagtacggc gcgcagcccg
      121 aactggcgca ccacgctccg accgacggtg gtcagacgct ggacctgcgg attgtccctg
      181 ccagagccag cgcgcagccg atccagcatg gccggtccct cgccgagcag gaacagcatg
      241 atcatcagga taaggaacag gtcgctaaga aaccctgcga tgccagcgag tacagtgcca
      301 agggcatcgg cgagggcact tggtttgacc acgtctcgaa cgtcaacgtt cgagagccca
      361 gaacgcttga tcagggcatc gatgttgtcc aactgtccgt taagttgcgt ggtgtagtac
      421 ccgatgcgct cgctgaacct gcttatagac acaccaagga tgaagaagag aaccgcgaag
      481 atcgcaccaa caagtatcag catgatcacg agggcaagcg gagaagggag cccgcggttc
      541 ttgagccagg catatacagg gctgaacaga agggaaagga cgatcgcaaa aaggatgggg
      601 ttgaggatcg gtgcagaaag gcgtatccca acgagcacga cgaccgttgc ggcgagtacg
      661 agcatcaccc gcaagaagga gggttggcgc agcgcgctgg tcacggcctc accgatgcca
      721 gtcctcttgc ctgccggctc gttcatggat tcgttcatga ctcagcctcc acgtcgctcc
      781 tggctctgtt gccaaactcg atacacagtc ttcccaagtt ctcctgcgtg gctcctcccc
      841 tctattcagc cacctcagat ggagcaggtg gcggcgacgc gacggcgggt tcttccgcct
      901 cttgcctcgt tcgccggtaa gccagtagtt cggtgactac tatgcggatg atctcggcta
      961 ccgggatggc taacagagcg ccagggatgc caagtagctg tccgcctact agcagggcga
     1021 tcagaatcac cagcccgttg agctggaccg ccttgctgta gacgatgggc tggaggaagt
     1081 tgctctcgac ctgctgatac accagtacga aaatgagcaa caccactgcc ttccacggct
     1141 cgacgaacag tcctactacg acgagtagcg ccccgccaag cgtcgcgccg accagcggta
     1201 cgaggtcgag caccgcgacc aagagtgcca gcggtgcggc gtagggcatg ccgagtacca
     1261 gcagcacgat gaaggtggtg ataccgcaga tcacgctgat tgcgaggttg ccggtgatgt
     1321 agccgctgat cgccccagcc gactgggtga gcaggcgtcg cacgagaggc cggtgtgcct
     1381 ctggaaagag ccctacaccg gcgttcacat accgctcgct gccaagcagc aggaagaagg
     1441 tgagcgtcag aacggtcgcc agagctgcga cgaagcctgc gaggccaacg gctacctgtc
     1501 cgctggccga gaagaggttc ctgagcaagt ctccaagctg ctgcctgagt tgggcgattt
     1561 ggtcgctgaa cctctcaaag agtccaccaa gcccgttttc cttggcgagc cccttgatgt
     1621 actccgtcgg tccctcgggg gcgttggccg cggtggtgac gaacttgatg aagccgttta
     1681 tctggtcgac gagcacgggc acgaaaatcc ccacgaccaa gagcagcgcc accagtacac
     1741 ccaggtaggt gagccctatg gcgagggggc gcttcatcag tctgtgtcgg cgctggagcc
     1801 agttgaccgc ggggtttagc acggcagcca ggaagaggga gatgacgaac cagccgatga
     1861 aagtgtgcac ctgccacagc aggaagagcg cacccagcac cacgactgcg gtcagtagag
     1921 cgatggcgac cgtacgcgcc acccgtgcgg gcggggcgcc ggcccaccca ccctcctgac
     1981 cgagcgctgg agcccttggc cccggagctg ccgctggagg tcgcacctgg ggttgtactt
     2041 gcgcttctac ttgtggtttt gtttgcttgt ccgcatgcgt ggccttttcc tcactcatat
     2101 ctatagcctc actgttctcc agggcgcgac tccctgggtg cgcccggtcg gtccgcgatc
     2161 gaagacctca tgtgccggga ttcctccatc tggacatctc accgtggttc atctgccctt
     2221 tggatcgctt gagggtagag tgtcttcgcg agtatgcacc tcgatccttt ctcccttctc
     2281 aggcgcgtct aaggacatct catcgacccg gcgggcgaac gcctcagcga gcggagcagc
     2341 cgtaacaccg tgcaagagca cgctgaggag caccgtcagc gccacaaccg cctccatctc
     2401 ctctcgccct gcaagttcgg gagcctctgc caccacgatc aggcccaaaa cgatcgaggc
     2461 aagtccccgc ggtccgaacc agcctatgaa cagcgtggag atgccgctca accgggtgcc
     2521 gaccacagaa actgcgaccg ggagcatgcg gactagggta aggctgatca gggcatacac
     2581 ggcaacctcc cagctcaagg gctggatcac tcctatcgcc agcacgccga agatgaagaa
     2641 tacgctcagg ttgagcaact gcccttctgc ttcggtgaag cggagcagcc gctccccgac
     2701 ccgttccacg gtcggcccta ccaccagacc cccaacaaag gccgcgataa agccattgcc
     2761 cccgatctgg tcggccagcg cccaggcaac gattgccaga gcaagtagag cgaggcgctg
     2821 gaacgaatcg gtcatccact ctcgcttaga ggccaggctc accagccagc ctcccacaag
     2881 ccctacgcct acgccgacga gagttcctaa accaatctgc tctacggcga agagtaacca
     2941 gtagcttgcg ggttgaagct cctcttcggc cactgccagt gtcaagaaca gcgcaaggaa
     3001 gggcacagag aggccatcat tgagcccggc ctcgacgtcc agggcctggc gcacgcggat
     3061 tgggactcgc gggttgctca ccaccgactg accgagcgcc gcgtccgtgg gggcgaggac
     3121 ggtgcccacg atggcggcct cccagaaagc gatatccgtg agcaacagta cagcggcagc
     3181 ggctccgagc gcgatggtga ggggcatccc cacacacaat aggcgcaagg gtagtccctc
     3241 gttccggcgt agggaggaga ggttgatgct cgctgcggcg gtaaacaaca cgatcgcgag
     3301 ggcgatctca cctaccagga gcacggtgtg gtcgtcgagc ctgatctcga ccaggcctag
     3361 gccccccgga ccgaggatgg cgccggccac gacaaagacg atgggcgctg tgaggatcgt
     3421 tccctccaag cgccttgaaa cgaggctaaa ggcgaaggtg accaaggcca gcacgacgag
     3481 gtcaaccacc gtaccctccg gtggtgcgag tgattcccgg actatacaaa ccaggtacgg
     3541 ccgtcacttg gaaacggcag acaatcgtcg accgagcaac cgtgtctgcg agggtcatga
     3601 gaatagctca ctcaaggttg cccatcgcag atctggacgg atctaccaat ggtctaaggg
     3661 tcgcgag
//
LOCUS       DATAIR010000198         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_328749, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000198 DATAIR010000000
VERSION     DATAIR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328749"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..1725)
                     /locus_tag="VHM69_04770"
     CDS             complement(508..1725)
                     /locus_tag="VHM69_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HEX2739738.1"
                     /translation="MLSYRRRSALRLFAMVFALAAGLVIVRGAMLSEAQAQTAPQYTF
                     TKVADSVEDDFAPFSFGCAAINTRGDIAFKAGRIASDGFNTIPGIYRANADGTLTTIA
                     EDQKKFVTIGFNPSMNDLGQVSFAARLDGGKKPDTEAILRGSGKKLTTIASTADEFRF
                     FGFDTSINNDGVVAFRAELDEEFNSDEGLFSGSGAGTTTHYLNSTDVSLDGQTARFGG
                     RDSRPSINSVGDIAFDEPIQPNFDSGIFVGREGVFRTIAAPDPDVFVQKPVLNDEGTV
                     AFYRSFFDEANQVFVDQIVTGNGGPLTTIADTGGAFSSFGFRPPSLNNEGDVAFHATL
                     DDFSTSGIFVGPDPVADRVIATGDTLDGRTVQNITFCEEGLSDTGELAFVAQLEDSST
                     PEGFRVAVFRATP"
     gene            complement(1921..2223)
                     /locus_tag="VHM69_04775"
     CDS             complement(1921..2223)
                     /locus_tag="VHM69_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2739739.1"
                     /translation="MYVVLWRFRPLRGRESEFERAYGPSGEWARLFQRGEGYLGTELF
                     RRSEDPREYLALDRWTSGAAYEEFRVRFSSAYRRLDRRLEELTEEEVPLGAFEALP"
     gene            2449..2844
                     /locus_tag="VHM69_04780"
     CDS             2449..2844
                     /locus_tag="VHM69_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739740.1"
                     /translation="MSKPQQFDDWPESIQPGFGPYRAVCERIVDGDTLYVFCDLGLSQ
                     YSYQSLRLLDVFAPELFSGTNREAGAEARDRLTSICPPGTKLQMYTEKDASTFGRYVA
                     TLEMADGTIVNEAMRAWLEEKGFTGGSGA"
     gene            2950..3420
                     /locus_tag="VHM69_04785"
     CDS             2950..3420
                     /locus_tag="VHM69_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX2739741.1"
                     /translation="MKGRRLFLSLVLAALVALALAGAASAARLDGADHGGRPLSTTMT
                     GAAEVPGPGDPDGSGTAKLTFNLGREEVCWEITAQDLTLPATAAHIHVGAAGTAPANN
                     VVVTLSAPDASGFTSGCTTVERELAEDILDNPENYYVNVHNSDFPGGAIRGQLS"
     gene            3577..>4185
                     /locus_tag="VHM69_04790"
     CDS             3577..>4185
                     /locus_tag="VHM69_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2739742.1"
                     /translation="MSSLPTGTVTFLFTDIEGSTRLWERSPEAMQGALIRHDQILREA
                     VEERGGYVFKTVGDAFCCAFTTATDALEATLKAQRALFAEEWNVEGGVRDRMALHTGV
                     AEERDGDYFGPPVNRVARLLHAAHGGQVLLSAATHEMVRDQLPEEVSLRDLGEQRLKD
                     LFRPERIFQLTASGHLGPNGAGKTTTIKLLMGLIHATRGSATI"
BASE COUNT          872 a         1235 c         1293 g          785 t
ORIGIN      
        1 ctgcgttggc aagcgtagca gtggcagacg gagacaccga tcggttctcc gcggacctcc
       61 gcctgaagct gaccacaact acaagatgcg atgcgaactg acatagggac tcccataggg
      121 tgacctgtaa cacccggctg caaatcttac cgctcgacgc agcataacat cccataaaga
      181 ggggccatgc cgatcagtct ttccgtaggc ttgtcctgca tcattttcct tgcaccatgt
      241 tgtcccaata actccaatgc ggagtttcag gaatggaagg aagatcgtcg aatcaccatt
      301 tagaggactg cccgggtgtc ggacttcgca tctccataga acccctacaa gcgaaggtct
      361 gagaaaaacg ccttctccgg gaggttgggt gaataggcgt tagagcaagg gccggggtcg
      421 cgtacgaccc cggcctctgc gtccctatct caacgcctcg tcgccgttgg gaccattcgt
      481 tggtctcgaa caacgacgct cccttcgcta cggcgtcgcg cggaagacgg ccacgcggaa
      541 gccctccggc gtactggagt cctccaactg cgccacgaac gccagctcgc cggtgtcgct
      601 caggccctcc tcgcagaagg tgatattttg aacggtccgg ccgtccaacg tgtcccccgt
      661 ggcgatcacc cgatcggcga ccggatcggg gcccacaaag atcccgctgg ttgagaagtc
      721 gtcgagggtt gcgtggaagg ccacgtcgcc ttcgttgttg agcgaggggg gcctgaagcc
      781 gaaggagctg aaagcgcccc ccgtgtctgc gatcgtcgta agcggaccgc cgttgccggt
      841 cacgatctga tccacgaata cctggttcgc ctcgtcgaaa aatgatctgt agaaggccac
      901 cgttccctcg tcgttgagca ccggcttctg gacgaagacg tccgggtctg gcgccgcgat
      961 cgtccggaag actccttccc tccccacgaa gatgccagag tcgaagttgg gttggatcgg
     1021 ctcgtcgaag gctatgtcgc cgacgctgtt tatcgacgga cgggaatcgc ggccaccgaa
     1081 gcgcgccgtc tgtccgtcga gggagacgtc ggtcgagttg agatagtgcg tggtggtccc
     1141 ggctcccgac cctgagaaga gaccctcatc agagttgaac tcctcgtcga gttcggctct
     1201 gaaagcgact acaccgtcgt tgttgatgga ggtgtcgaac ccgaagaatc tgaactcgtc
     1261 cgccgtgctg gcgatggtgg tcagtttttt gccgctaccg cgcaagatgg cctcggtgtc
     1321 gggcttctta ccgccatcca gcctcgccgc gaaggagacc tgcccaaggt cgttcatcga
     1381 ggggttgaaa ccgatagtaa cgaacttctt ctggtcttcg gcgatggtcg tcagggttcc
     1441 gtccgcgttg gcccggtaga taccggggat ggtattgaac ccgtcggagg ctatgcgtcc
     1501 cgctttgaaa gcgatgtccc ctcgcgtgtt gatcgcggcg catccgaaac tgaagggagc
     1561 gaaatcgtcc tcgacgctgt ccgccacctt ggtgaaggtg tattgcggag cggtttgtgc
     1621 ctgcgcctct gagagcattg ctccgcgtac tatgaccagt cctgccgcca gggcgaagac
     1681 catcgcaaac aaacgcaagg cggagcgtcg tcggtacgaa agcatagata cctccttctc
     1741 agggggcgcg tacgtaagtt gcctttccgc attcccctca tcagcgtacc cacgtctata
     1801 cgtacaagaa gcttcttgcg gattggctgg atctcgaaac ttactcaacg aagaacactt
     1861 cttccacgca ttcgggtgaa gaaggacaga aatggaaggg ctagacagcg atcggagcct
     1921 ctaagggagt gcctcgaaag cgcccagggg cacctcttcc tctgtgagct cttccaaccg
     1981 acggtcgagc cgtcgatatg cgctgctgaa gcgaactcgg aattcttcgt aggcggcgcc
     2041 agaggtccag cggtccagag ccaggtactc cctgggatct tcggagcgcc ggaacagttc
     2101 ggtgccgagg tagccctcgc cgcgctggaa cagccttgcc cactccccgg atgggccgta
     2161 ggcccgctcg aattcgctct cccgacccct gaggggccgg aaccgccaga gcacgacata
     2221 catggtcagc ctcctcaggt tgaccttagc ctacgcttcc tcacgggcga cttctacggt
     2281 gaccttcaca gaaggctctg gggcgaagtt cgcagagaac cctcccctgg caattgagtg
     2341 catagagcct gcgactcatc gtcctggcta cactacaatg tgcaggcacg aaggagtgac
     2401 cgaggaaagc agaagcatgt attgtcagct accgacgggg cggtaccgat gagcaagccg
     2461 cagcaattcg acgactggcc agagtccatc caaccgggct tcggcccata cagagctgtg
     2521 tgcgagagga tcgtagacgg tgacacgctc tacgtctttt gtgacctggg actgagccag
     2581 tactcgtatc agtccttgag actactggac gtgtttgctc ccgagctatt ctcaggcacg
     2641 aacagggagg ccggagcgga ggcgagagac cgcctgacct caatctgtcc gccagggacg
     2701 aagctacaga tgtatacaga gaaggacgct tccactttcg gtcgctacgt cgcaacgctc
     2761 gagatggccg atggcaccat agtcaatgag gcgatgaggg cgtggcttga ggagaaaggc
     2821 ttcactggag gttcgggagc atgagcaact aagactcttg actttgcgga agtgaacaag
     2881 gcgcgatcca ctacggactt ccggtacgta ttaaatattg caagggaaag cgtctcagga
     2941 ggtattgagt tgaagggaag acgactgttc ttgtcgttgg tcctggcagc gctggtggcc
     3001 ctggcgctcg cgggggcggc atccgccgcc aggctagacg gtgctgacca tggaggacgc
     3061 cctctctcga ctacgatgac cggtgccgcc gaagtaccag gtcccggcga tcccgacggc
     3121 agcggcacgg cgaagctgac tttcaacctt gggcgggaag aggtctgctg ggagatcacg
     3181 gcgcaggacc tcacgctacc cgccacggcc gcgcacatcc acgtaggtgc agcaggcacg
     3241 gcgccagcca ataacgtggt cgtgacactc tcggcgcccg acgcgagtgg attcaccagc
     3301 ggctgcacga ccgtggagcg cgagctggcc gaggacatcc tcgacaaccc cgagaactac
     3361 tacgtcaacg tccacaatag cgacttccca ggcggggcga taaggggaca gctctcataa
     3421 cgcgagcacc agcggagcga gggcggcaaa tcccgaggac gctgcaaagg cgaagctcgt
     3481 agtaggtgtg ctacgaggtt gggtgaatac gggtaaggag aaggtccgct agtagctgct
     3541 gctgctatat tgtcccctta gaggaaaagg gagccaatga gcagcctacc cacgggcacc
     3601 gtcaccttcc tgttcaccga catcgagggc tccaccaggc tctgggagag gagtcctgag
     3661 gcaatgcagg gggccctcat ccgccacgac cagatcctga gggaagccgt cgaggagcgt
     3721 ggcggttacg tcttcaagac cgttggagat gctttctgct gcgcgttcac taccgccacg
     3781 gatgctctcg aagcaaccct caaggcccaa cgggcgcttt tcgctgaaga gtggaacgtc
     3841 gaaggcggcg tgcgggacag gatggccttg cacacgggtg ttgccgagga gcgtgatggc
     3901 gactacttcg gtcctcctgt caacagggta gcgaggctcc tccacgctgc gcacggaggg
     3961 caggtactgc tctcagcggc tacccacgag atggtgcgcg atcaactgcc tgaggaagta
     4021 agcctcaggg acctggggga gcagcgcctc aaggacctct tcaggccgga gaggatcttc
     4081 cagcttacgg cgtccggaca cctcggcccg aacggggcgg ggaagaccac cacgatcaag
     4141 ctcctgatgg gcctgatcca cgcgacgaga ggatcggcca cgatc
//
LOCUS       DATAIR010000199         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_381593, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000199 DATAIR010000000
VERSION     DATAIR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381593"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VHM69_04795"
     CDS             <1..461
                     /locus_tag="VHM69_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2739743.1"
                     /translation="NEEEVSRQSDVEMSEDDNVLVQGVSGDENALGYFGYSYYENNLD
                     TLKALAVDGVKPTEDSIRSGEYVLSRPLFIYVSTDALKNNKAVKPFVDFYVSEQNLDR
                     LVEAAKYVTLPSSLAQESRAQYEDRTTGTVYTEEGEPKGGDLETALKQSQ"
     gene            557..1489
                     /gene="pstC"
                     /locus_tag="VHM69_04800"
     CDS             557..1489
                     /gene="pstC"
                     /locus_tag="VHM69_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX2739744.1"
                     /translation="MAIEAGGTRSGTGAWKRNSRTRELRERVIKLLLTACAYLSIFTT
                     VGIVIVLFEETARFFVEVSPVEFLFSTRWAPQQGSFGVLPLVYGTLMATIIAIAVALP
                     IGLLTAIYLSEYAPKALRKWLKPGLEILAGVPTVVYGYFALTFITPFFRDGIFSGIES
                     FNALAAGIIMGVMIIPTVASVSEDAIYAVPRSLREGAYALGATRRETATKIVVPAALS
                     GIVAAVILGVSRAIGETMIVTIAMGSQPNFGGSPLESMQAMTAYIVQVVGGETPRESV
                     TYKSIFAVGSALFLMTLALNLASYWFVRRYRETY"
     gene            1489..2382
                     /gene="pstA"
                     /locus_tag="VHM69_04805"
     CDS             1489..2382
                     /gene="pstA"
                     /locus_tag="VHM69_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX2739745.1"
                     /translation="MAQQKGGQVFKPAMSTRYGLDRAFTALVTVAALVGIAVLVVLLV
                     DVIRDGSTMLSWQFLTSFPSQIFPENGGIYPALIGSVWLLGLTALISVPLGLGAAVYL
                     EEYAEDTRINRIIEINISNLAGVPSVIYGLLGLGIFVQLLAPVTGGTSVLTGALTLSL
                     LILPVIIVATREALRAIPGDIREGGYALGATKWEVIWSHLLPMALPGALTGIILALSR
                     AIGEAAPLLVVGVSLYQTYATAGPFDGYMALPTQIYDWISRPQQIFQDSAAAGIVVAM
                     AVLLLANSFAIWLRNRHQQRS"
     gene            2416..3285
                     /gene="pstB"
                     /locus_tag="VHM69_04810"
     CDS             2416..3285
                     /gene="pstB"
                     /locus_tag="VHM69_04810"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX2739746.1"
                     /translation="METEKTGRGRKVSEVSRDQREGRQEGDGAPPGDGAALGMKVQDL
                     SAFYGSFKALTDVSMDIQANKVTALIGPSGCGKSTFIRCLNRMHEVIPGARAEGHVML
                     GEKDIYAKTADPVNIRRRIGMVFQKPNPFSKTIYENVAWGARINGYRGDMDELVERSL
                     KQAALWEEVKDRLKESGHGLSGGQQQRLCIARTLAVEPEVILMDEPASALDPVSTQKI
                     EDTMDQLKESYTVVIVTHNMQQAARISDYTGFFYIQNMGDPGRLWEFDDTEKMFSNPD
                     RKETEDYVTGRFG"
     gene            3289..3987
                     /locus_tag="VHM69_04815"
     CDS             3289..3987
                     /locus_tag="VHM69_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX2739747.1"
                     /translation="MPRETFQQELDGLIQDLLGLGKEVEGSLENMVVAMEGRDADVAG
                     RELGVDIRYKAWGMEIERECMILQARQAPVARDLRLLYTVQSVTNHLVRSGTLSEHIC
                     HAIVDTAECERDEDLETALVEMARTARNVFNEGLDVFENRDIDRARDLQAKDDKVDLL
                     YSEAMNLIANPSDGRGGDPEWRMRAALMVHYLERISDHGVDIGGSTVFLVTGERIEDA
                     MRQYLDRDVDEDGI"
     gene            4060..4509
                     /locus_tag="VHM69_04820"
     CDS             4060..4509
                     /locus_tag="VHM69_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2739748.1"
                     /translation="MRLTQKSKYAVRALMELALDERDCPLGVAEIARRQRIPERFLEQ
                     IFGELRRANIIESRRGAHGGYRFAMPTEEISVLDIVEILDGEIRPARCSAGGVCYISD
                     APLCATSKVWDEARQALESVFGRYSIAQLAAAELEERAAHETAPAAR"
BASE COUNT          891 a         1432 c         1542 g          835 t
ORIGIN      
        1 ggaacgagga ggaagtcagc cgccaatccg acgttgagat gagcgaggac gacaacgtgc
       61 tcgtgcaggg tgtctcgggc gacgagaacg cgctcggtta cttcgggtac tcgtactatg
      121 agaacaacct cgacaccctc aaggcgctcg ccgtcgacgg cgtaaagccc accgaggatt
      181 ccatccgctc cggcgagtat gtgctctccc gccccctgtt catctacgtg agcaccgacg
      241 cgctcaagaa caacaaggcg gtcaagccct tcgttgactt ctacgtctcg gagcagaacc
      301 tcgacaggct cgtcgaggcc gcgaagtacg tgacgctgcc gagcagcctg gcgcaggaat
      361 cgcgggccca gtatgaggac cgcaccacag gcaccgttta caccgaggaa ggtgagccga
      421 agggcgggga cctggagacg gcgctcaagc agtcgcagta accgtttgta cctggatggg
      481 tctgcttgta acatatgggg gccggcacaa tagccggtcc tcataattat gatcacgagg
      541 tttcggagga ttttcggtgg cgattgaagc ggggggcacg cggtcaggga cgggtgcctg
      601 gaagcgcaac agccgcacca gggagctaag ggagcgggtc ataaagctcc tgctcacggc
      661 ctgcgcgtac ctgtcaatct tcacgacggt cggtatcgtc atcgtcctct tcgaggagac
      721 cgcccggttc ttcgtggagg tctctcccgt cgagttcctc ttctcgacgc gctgggcgcc
      781 gcaacagggc agcttcggtg ttctgccgct cgtctacggc acgttgatgg ctacgattat
      841 cgcaatagcc gtggcgctcc cgatcgggct gctcacggcc atctacctca gcgagtacgc
      901 cccgaaggcg ctaaggaagt ggttaaagcc gggccttgag atcctggccg gcgtgcccac
      961 cgtggtctac gggtacttcg ccctgacgtt tatcacgcca tttttcaggg acggcatctt
     1021 ttcgggaata gagtcgttca acgcgctcgc cgcgggcatc atcatgggcg tcatgatcat
     1081 ccctacggtg gcctcggtca gcgaggacgc catctacgcg gtgccgcggt cgttgcggga
     1141 gggcgcctac gcgctcggcg cgaccaggcg cgagacggcg acgaagatag tggtgccagc
     1201 ggccctctcc gggatcgtgg ccgcggtaat cctcggcgtc tcgcgcgcca tcggggagac
     1261 gatgatcgtg actatagcga tgggctctca gcctaacttc ggcggttccc cgctagagag
     1321 catgcaggcg atgacggcct atatcgtgca ggtggtcggc ggcgagacgc cgcgcgagtc
     1381 ggtgacctac aagtccatat tcgccgttgg gtcggcgttg ttcctgatga cccttgccct
     1441 caacctcgcg agttactggt tcgtccggcg atacagggag acctactaat ggcgcagcag
     1501 aagggcggac aggtgttcaa gcctgcaatg tccaccaggt acggcctgga cagggccttt
     1561 accgcgctcg tgacggtggc tgcgctcgtt ggcatcgccg tcctcgtggt gctcttggtg
     1621 gatgtgatcc gggacggctc cacgatgttg agctggcagt tcctgacgag tttcccgtcc
     1681 cagatcttcc ccgagaacgg tggcatatat cccgcgctga tcggatctgt atggctgctc
     1741 gggctgaccg ccctgataag cgtgccgctg gggctggggg ccgccgtgta cctcgaagag
     1801 tacgccgagg acacacgcat caaccggata atcgagatca acatctccaa cctcgccggg
     1861 gtgccttctg tgatctacgg actcctcggc ctcggcatct tcgtgcagct cctggcgccc
     1921 gtaacgggcg gcacgagcgt gctaaccggc gcgctcacgc tgagtttgct catccttccg
     1981 gtcatcatcg tcgcgaccag ggaggctttg cgggctatcc ctggcgacat cagggagggt
     2041 ggctacgcgc tcggggcgac gaagtgggag gtgatctgga gccatttgct acctatggcg
     2101 ctgccgggcg ccctcacggg catcatcctg gctctctcgc gggccatcgg cgaggcggcc
     2161 ccgctcctcg tcgtcggggt ctccctgtac cagacctacg ccaccgcagg accgttcgac
     2221 ggttacatgg cgctgccaac ccagatatac gattggatca gccgcccgca gcagatcttc
     2281 caggactccg ccgccgccgg catagtggtt gcgatggcgg tgctcttgct ggcgaactcg
     2341 ttcgcgatct ggctgcgtaa caggcatcag cagcggtctt gatggcggtt tgtaaaggag
     2401 aaaggtgtta gcctaatgga gactgagaag accgggaggg gacggaaggt gtccgaggtt
     2461 tcgagggacc agcgcgaggg gcgacaggag ggcgacggcg cgccccctgg cgacggtgcc
     2521 gccctcggca tgaaggtcca ggacctctcg gcgttctatg gttccttcaa ggccctaacg
     2581 gacgtgagca tggacataca ggccaacaag gtgacggcac tcatcgggcc ttcggggtgc
     2641 ggaaagagca cgttcataag gtgcctgaac aggatgcatg aggtgatccc tggcgcgcgc
     2701 gccgaagggc atgtgatgct cggcgagaag gacatctacg ccaagaccgc tgaccctgta
     2761 aatatccggc gccgcatcgg catggtcttc cagaagccca acccattctc caagaccatc
     2821 tacgaaaacg tcgcgtgggg ggccaggatc aacggctacc gcggcgacat ggatgagctt
     2881 gtcgagcgct ctttgaagca ggcagccctc tgggaggagg tcaaggaccg cctcaaggag
     2941 agcgggcacg ggctctcggg cggccagcag cagcgcctat gcatcgccag gacgctcgcg
     3001 gttgagcccg aagtgatcct catggacgag ccggcgagcg ccctcgatcc ggtctcgacc
     3061 cagaagatag aggacaccat ggaccagctc aaggagagct acacggttgt catcgtgacg
     3121 cacaacatgc agcaggcggc gcgcatctcc gactacacgg gtttcttcta catccagaac
     3181 atgggcgacc ccgggcggct ctgggagttc gacgatacgg agaagatgtt ctcgaaccct
     3241 gatcgcaagg agacggagga ctacgtgacg ggccggttcg gctagagtat gccgcgggag
     3301 accttccaac aggagctcga cgggctgatc caggacctgc tcggcctcgg caaagaggtc
     3361 gaggggtctc tcgagaacat ggtcgtggcg atggagggac gtgacgccga cgtggccgga
     3421 cgagagctcg gggtggacat ccgctataaa gcgtggggca tggaaatcga gagggagtgc
     3481 atgatcctgc aggcacgcca ggcccctgtc gccagagacc tcaggctgct gtacaccgtg
     3541 cagtccgtga cgaaccacct cgtgcgctcg ggcaccctca gcgagcacat ctgccacgct
     3601 atcgtcgaca ccgcagagtg cgagcgggac gaggatctcg agaccgctct cgtagagatg
     3661 gcgaggaccg cccgcaacgt attcaacgag ggcctcgacg tgttcgagaa ccgcgacatt
     3721 gaccgcgccc gcgacctgca ggcgaaggac gataaggtcg acctcctcta ctcggaggcg
     3781 atgaacctga tagcgaaccc ttcggacggg cgcggtggcg acccggagtg gcggatgcgg
     3841 gcggccctga tggtccatta ccttgaacgc atctccgacc atggggtgga catcggcggg
     3901 agcacggtct tcctggtgac gggggagcgc atcgaagacg ccatgcgcca gtacctggac
     3961 cgcgacgtgg acgaggacgg catctaaaaa cggcggttgt cgcctgtgca cttctgatac
     4021 aatctatcgg aagcggttcc tgcaggcggt gattattcct tgagacttac tcagaagagc
     4081 aaatatgcgg taagggcgtt gatggagctc gcgctcgacg agcgcgattg cccgctgggg
     4141 gtggccgaga tcgcgcggcg ccagcgcatc cccgagaggt tcctggagca gatcttcggc
     4201 gagcttcgac gggcgaacat tatagagagc cgccgcgggg cgcacggagg ataccgcttc
     4261 gcgatgccca cggaagagat ctccgtcctc gacatcgtcg agatcctgga cggtgagatc
     4321 cgtcccgccc gctgctccgc cggcggggta tgctacatct ccgacgcccc cctttgtgcc
     4381 acgagcaagg tctgggatga ggcccgccag gccctcgaga gcgtcttcgg gcgttacagc
     4441 atcgcccagc tcgccgccgc cgagctcgaa gaacgcgcgg cccacgagac cgccccagcc
     4501 gcccgctgac gacttctgga tagactgcga gcccatctgc taggccgtac tgggctggcc
     4561 gaatccacgg tcgaggtcgg agacaggacc tatcgcatca cgcatcccgt ctccgccgac
     4621 gacctgatcg acgaggaaga cttcgaccgc gacgagcggc tcccttactg ggccgagctc
     4681 tggccgagcg ccatcgccct
//
LOCUS       DATAIR010000200         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_427747, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000200 DATAIR010000000
VERSION     DATAIR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427747"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..597)
                     /locus_tag="VHM69_04825"
     CDS             complement(283..597)
                     /locus_tag="VHM69_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX2739749.1"
                     /translation="MAQYAWVLEVRPGYEEEYKRRHDEIWPEMLDALKEAGIRNYSIF
                     RHGLTLFGYFETDDLEKTQKYLAESETNKRWSEWMDPIMKVDIDPTTDFPYLLPLQWH
                     MD"
     gene            complement(635..>2053)
                     /gene="rhaD"
                     /locus_tag="VHM69_04830"
     CDS             complement(635..>2053)
                     /gene="rhaD"
                     /locus_tag="VHM69_04830"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX2739750.1"
                     /translation="EFVASRAGEPFGGQVVEPPAPDRRETLLAEILPALRGELSSGSD
                     ESSHKILRVDHTSEEVLTFVCGRDSKELSQVGAACPDHLVRTKVRPLWVDFDPEREGV
                     EELKERLREDVTRYREDYEAYFSRHEEADEEMFDPNPRVVIIAGLGLVAAAKNAKEAN
                     LSRDFAYRAINVMRGAHALGGYVSLTEEESYAVEYWPLELYKLTLAPPPDELAGRIAL
                     VTGGAGGIGSAVARSLASRGACIVVCDLDEEGASEVAESLPEPGIPARADVTDENEVA
                     GAYRRAILEFGGVDVVVSNAGLASSAPIEETSVEMWDKNHAVLAKGYFLVAREAFRIM
                     KDQGVGGSIVFVASKNSMAAGTNASAYSSAKAAELHLARCLAEEGGPVRIRVNTVNPD
                     AVLQGSRIWGSDWREERARAYGIEPDELEDYYRKRNVLKVNVLPENIAEAVLHFASEA
                     RSSRSTGNVLNVDGGVKDAYPR"
BASE COUNT          333 a          663 c          626 g          431 t
ORIGIN      
        1 agaggccaga ctggcctccg gcatggtgct tgtcaagatg tgcttgttcc tctctatcgc
       61 tgtgtagtgg tcgccaccat aaggagcgta cgtcgcgggc taccactcaa cgggagagga
      121 caggcacgag gcacaaaaaa agagatgctc ttcggacatc cgacatctcg atgatcgtgg
      181 acgtatgttc tttatcgcgt agcgtcccga gtataccgga cgcgcgtcct taatcaaagt
      241 agcggacgcc ctaacctggt tcggtcgccg tcacgggctg gatcaatcca tatgccattg
      301 cagggggagg aggtacggga agtcggtcgt ggggtcgatg tctactttca tgatggggtc
      361 catccactcg ctccaccgct tgttggtctc gctctcggcc aggtacttct gggttttctc
      421 aaggtcgtcg gtctcgaagt agccgaagag ggtgaggccg tggcggaaga tagagtagtt
      481 acggatgcct gcctctttga gtgcgtcgag catctcgggc cagatctcat cgtgccgcct
      541 cttgtactct tcctcatacc ccggcctgac ctccaggacc cacgcgtact gcgccatacg
      601 tccctccttc ggcaactccg tccggctgtc ccctctatct gggataggcg tctttcacgc
      661 ccccgtcgac gttcagtacg ttgcccgtgc tccgcgagga ccgtgcctcg gaggcgaagt
      721 ggagcacggc ctcggcgatg ttctcgggca gcacgttgac tttgagcacg ttgcgcttac
      781 ggtagtaatc ttcgagctcg tcaggctcta tgccgtaggc gcgggcgcgc tcctcgcgcc
      841 agtctgagcc ccagatcctg gacccctgca ataccgcgtc ggggttgacg gtgttgaccc
      901 tgatgcgcac cggccctcct tcctcggcca gacaccgcgc cagatgtagc tcggccgcct
      961 tggcggagga gtacgcgctg gcgttcgtgc ccgccgccat ggagttcttc gaggcgacga
     1021 agacgatgct gccaccaacg ccctgatcct tcatgatgcg gaaggcctcg cgcgccacga
     1081 ggaagtaacc tttcgcgagg acggcgtggt tcttatccca catctcgacg ctcgtctcct
     1141 cgatgggagc gctggaggcg aggcccgcgt tcgagacgac gacgtctacc ccgccgaact
     1201 cgaggatcgc gcgccggtag gcgccggcta cctcgttttc atccgtcacg tctgccctcg
     1261 cagggatgcc aggctcagga aggctctccg cgacttcact tgcgccctcc tcgtccaggt
     1321 cgcagaccac gatgcaggcc ccgcgcgacg caagcgacct ggcgacggcg ctcccgatgc
     1381 cgcctgcgcc gcccgtgacc agggcgatcc tgcccgcgag ttcatccgga ggaggggcga
     1441 gggtgagctt gtagagttcg agcggccagt actcgaccgc gtaggattct tcctctgtaa
     1501 gtgagacgta gcccccgagg gcgtgggcgc cgcgcatgac gttgatcgcc ctgtaggcga
     1561 agtccctgga gaggtttgct tctttggcgt tcttcgcagc ggcgacgagt ccgagtcccg
     1621 ctatgatgac gaccctcggg ttcgggtcga acatctcctc gtccgcctcc tcgtggcgcg
     1681 agaagtatgc ctcgtagtct tccctgtagc gcgtgacgtc ctcgcggagc ctctccttca
     1741 gttcctcgac gccttctctc tcaggatcga agtccaccca gaggggtctg accttcgtcc
     1801 tgaccaggtg gtccgggcag gccgctccga cctgggagag ctccttcgag tcgcgtccgc
     1861 aaacgaaggt gagaacttct tctgatgtgt gatccaccct cagtatcttg tgggacgatt
     1921 catctgaccc cgacgagagc tccccgcgca gcgcgggcag aatctcggcg agcagagttt
     1981 ctcttctatc tggtgccggc ggctcgacca cctgtccgcc gaagggctca ccggcccggc
     2041 tcgcgacgaa ctc
//
LOCUS       DATAIR010000201         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_190108, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000201 DATAIR010000000
VERSION     DATAIR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190108"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..934
                     /locus_tag="VHM69_04835"
     CDS             134..934
                     /locus_tag="VHM69_04835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX2739751.1"
                     /translation="MPQTSASVFDLEQPRTDGMPIHPAHKQTGYSYLLHRHHEDEYRP
                     DESGPRTGAAGVLICSEHTGTHIDALCHQSDALVLCGGVPVEEVQSPRGFTRHGVEEI
                     PPIVASGVLLDVAAKDGVESLEPGRVVTAADLEECCERQGVIVEAGSVALVRLGNARF
                     WDDEERYLAGPGMDGSAAHWMAGRGVLAVGADNMAWDAPGYKDPELGLQLMGHLILLA
                     RQGIYIIENLALDELAASGHYRFEFVCTPLKLVGATGSPVRPVAVVSG"
     gene            complement(931..2082)
                     /locus_tag="VHM69_04840"
     CDS             complement(931..2082)
                     /locus_tag="VHM69_04840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2739752.1"
                     /translation="MEERSKLPLDGVRVLDLSRVLAGPYATMVLADLGADVLKVEHPE
                     RGDDTRHWGPPFAGNESAYFLSVNRNKRSIGVDLKDPEGLERVKKLAAGADVVIENWR
                     RGALDRLGLGYEVLSENNPDLIYCSITGFGPGPDEDRPGYDFLVQARGGVMGITGQPG
                     GEPTKVGVAISDIVCGLFACNAILAALYRRSATREGARIEVPLFESTLGWLANRGQEY
                     LVSGDDIGLIGNAHPSIVPYQTFDASDRPLVVAVGNNTQFANLCRVIGRPELAEDERY
                     TTNPDRVANRETLIQQLQEEFRKRTADEWAEEIRASGVPCGPVNTLANVFTDEHVLAS
                     GMLQDLDHPSAGPLKMLASPVLIDGERLPIRRPPPTLGQHTEEAHDDWS"
     gene            2198..>2443
                     /locus_tag="VHM69_04845"
     CDS             2198..>2443
                     /locus_tag="VHM69_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2739753.1"
                     /translation="MAVTRAGRNSDTGAFGTPEAGDFLNIDAHLSEEEIGVRERVRSF
                     VHERIRPNIKEWYDEAVFPRDIVPGFAELDLLGMHLSG"
BASE COUNT          429 a          750 c          809 g          455 t
ORIGIN      
        1 ggtgctcatg agatcacctc ttattcgttc ggcttcctca gacaaaaccc taacatctct
       61 cgccatttcc ggcacgatgc tggcgcgtgg cggggaagag aattaacctg aatggagagc
      121 ggaagggagc tttatgccgc aaaccagcgc cagcgtcttc gaccttgagc aaccacgcac
      181 cgacgggatg ccgatccacc cggcccacaa acagaccggc tactcctacc tactgcatcg
      241 ccaccacgag gacgagtacc gtccggacga gtccggccca cgtaccggtg cggcgggtgt
      301 cctcatctgc agcgaacaca cgggcaccca catagacgcc ctctgccacc agtcagacgc
      361 cctcgtgctc tgcggcggtg tccccgtcga ggaggtgcag tccccgcgtg gcttcacgcg
      421 gcacggcgtc gaggagatac cgcccatagt agcctccggc gtcctcctcg acgtcgccgc
      481 gaaggacggc gtggagtccc tggaaccagg tcgcgtcgtc acggccgcag acctcgaaga
      541 gtgctgtgaa cggcagggcg taatcgtcga ggccgggagc gtggcgctcg ttcggctcgg
      601 caacgcccgc ttctgggacg acgaggaacg ctatctggcg ggaccaggga tggatgggag
      661 cgcggctcac tggatggccg ggcgcggcgt gctcgcggtc ggggcggaca acatggcgtg
      721 ggacgcgcca ggatacaaag accccgagct ggggctccag ctgatgggac acctgatcct
      781 tttggccagg cagggtatct acataatcga gaacctcgcg ctcgacgagc tggctgcgtc
      841 aggacattac cgcttcgagt tcgtatgcac tcccctgaag ctcgtcggcg cgaccggctc
      901 ccctgtcagg ccggtggccg tcgtttcggg ctagctccag tcgtcgtgtg cttcttcagt
      961 atgttgaccc agggttggtg gagggcgccg gatcggcagg cgttcgccgt cgatgagtac
     1021 tggcgaggct agcattttca ggggtccggc tgacggatga tcaaggtcct gtagcatgcc
     1081 cgaggcgagg acgtgctcgt ccgtgaagac gttggccagg gtattgaccg ggccacacgg
     1141 cacgcctgag gcccggatct cctccgccca ctcgtccgca gtccgtttgc gaaactcttc
     1201 ctgtagctgc tggatcaacg tttcccggtt cgccacgcgg tcggggttgg tggtgtagcg
     1261 ttcgtcttct gccagctcgg ggcggcctat gaccctgcac aggttggcga actgggtgtt
     1321 gttgccgact gccacgacca gtggtctgtc cgaggcgtcg aaggtctggt aggggacgat
     1381 ggagggatgg gcattgccta tgaggccgat gtcatcgccg ctgacgaggt attcctgccc
     1441 gcggttggcg agccagccga gcgtggactc gaagagcgga acttcgatgc gcgcgccctc
     1501 cctggtcgcc gagcgcctgt agagggcggc caggatcgcg ttacatgcga acaggccgca
     1561 cactatgtcc gagatcgcga ctcccacctt cgtcggttcg cctcctggct ggcccgttat
     1621 gcccatgacc ccgcccctcg cctgcaccag gaagtcgtag ccaggacggt cctcgtccgg
     1681 gccaggtccg aaacccgtga ttgagcagta gatgagatcc gggttattct cactcagcac
     1741 ttcgtagccg aggccgagcc tgtcgagggc gccgcgtctc cagttttcga tcaccacatc
     1801 agcccctgcc gccagcttct tgacacgctc caggccctca gggtctttga ggtcaacgcc
     1861 aatagagcgc ttgttgcggt tgacggagag gaagtaagcc gactcgttgc cagcgaaggg
     1921 cggtccccag tggcgggtgt cgtccccgcg ctcgggatgt tcgaccttca gcacgtcggc
     1981 gccgaggtcg gccagaacca tcgtagcata aggtcccgca aggacgcgcg agaggtcgag
     2041 gactctgacg ccgtcgaggg gcagcttcga gcgttcttcc atgctgccag tgtagcgtgg
     2101 ggtatccaca gcggccagag taggtgttcg tgacgcgcgg atgcgcgctg tggaataatc
     2161 cgtggtcgat gggcggactc ttaagagagg tgagacgatg gctgtgacga gggccgggcg
     2221 aaactctgac actggcgctt tcggtacccc ggaggcggga gacttcctga acatcgacgc
     2281 ccacctctcc gaagaggaga taggcgtcag ggagagggtc cgttcctttg tacacgagag
     2341 gatcaggccg aacatcaaag agtggtacga cgaggccgtc ttcccgaggg acattgtgcc
     2401 agggttcgcg gagcttgacc tgctcgggat gcacctctcc ggc
//
LOCUS       DATAIR010000202         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_381659, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000202 DATAIR010000000
VERSION     DATAIR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381659"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VHM69_04850"
     CDS             complement(<1..516)
                     /locus_tag="VHM69_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2739754.1"
                     /translation="MSLKVRIIPCLDVDAGRVVKGTNFTNLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEQRDTAATLARRAAEEVFVPYTIGGGVRTADDMRAMLRAGADKVS
                     VNSAAVRNPDLITQGAERFGSQCVVLSVDVKRRSTSPGWEVYLNGGRVNTEMDAVSWL
                     VEGEKRGAGEFV"
     gene            complement(513..1151)
                     /locus_tag="VHM69_04855"
     CDS             complement(513..1151)
                     /locus_tag="VHM69_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2739755.1"
                     /translation="MNVALPSTQRYGREEVRTGGAKRPVPSAVLRSEGFDGDGQADLL
                     NHGGAEKAVCVYPFDHYAHWERVFGHRLEWGAFSENLTVSGAVETEVCIGDVFRVGDT
                     VVQVSQPRTPCSKLAGKNGQRLLTKWVGKTGYTGYYLRVLSEGLVRSGDAFERVENHP
                     DLISIADVNDVIYGRSEDAGLIERLANLPEFGADGRALFVERLARMREKSLP"
     gene            complement(1242..>1592)
                     /locus_tag="VHM69_04860"
     CDS             complement(1242..>1592)
                     /locus_tag="VHM69_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739756.1"
                     /translation="NKESRASALRSAIEVLWDTPGLRLRTGNLSSDISAPENVDVSKL
                     DVSASMSHAELDRTVSLVEGTLAAYTVHRHWKGRDGSMVVGAGDEGSVLLPAKTALRE
                     RLAEECGTASVPYV"
BASE COUNT          261 a          553 c          471 g          307 t
ORIGIN      
        1 gacaaactcc ccggcccccc gcttctcgcc ctcgactagc cagcttaccg catccatctc
       61 tgtgttgacc cgccctccgt tcaggtagac ctcccagcct ggggaggtac tccggcgctt
      121 cacgtcaacg ctcaggacga cgcactggct cccgaagcgt tccgctcctt gtgtgatgag
      181 atccgggttc ctcacagcgg cgctgttgac ggagaccttg tcggccccgg cgcgcagcat
      241 ggcgcgcatg tcgtccgccg tcctgacacc gcctcctatt gtgtatggga cgaatacctc
      301 ctcggcggcg cggcgggcga gcgttgctgc tgtatccctc tgttcgtggg aggctgtgat
      361 gtcgtagaag acgatctcat cggccccctc cctgtcgtag agggccgcaa gctccacggg
      421 atcaccagcg tccctcaggt tcgtgaagtt agtccccttc acgacacgcc cggcgtccac
      481 gtcgagacaa ggaattatgc gcaccttgag gctcacggga ggcttttctc tcgcatgcgg
      541 gccaggcgct caacgaagag ggcgcgcccg tcggcgccga actcggggag gttggcgagg
      601 cgttcgatca gtccagcgtc ctccgaccgc ccgtagatga catcgttcac gtcggcgatg
      661 gagatgaggt caggatggtt ctcgacgcgc tcgaaggcgt cgcccgatct cacaagacct
      721 tccgacaaga cccgcaggta gtaccccgta tagcccgtct tccccaccca cttcgtcagc
      781 agcctctgcc cgttcttgcc ggccagcttg ctgcaaggcg tccgtggctg gctgacctgg
      841 accacagtgt ctccaaccct gaacacgtcg ccaatgcaga cctcggtctc tacggcgccc
      901 gagacggtca ggttctctga gaaggccccc cattccaacc gatgtccgaa caccctctcc
      961 cagtgcgcgt agtggtcgaa ggggtagacg cagacggcct tctcggcccc accgtggttc
     1021 agcaggtcgg cctgcccgtc gccgtcgaat ccttcggaac gaagcacggc actcggcacg
     1081 ggcctttttg ctccacctgt cctcacctct tctcgcccgt agcgctgcgt cgagggtaag
     1141 gctacgttca acgaaacgac cttcacagcc gtcctggtcc tttcagggta gcttgccgtt
     1201 acagggtagg gtacgaccgg agaacttgcc aacaccgggg gctacacgta gggtacgctg
     1261 gctgtgccgc actcttcggc caggcgctcg cgcagggcgg tcttcgcggg cagcaggacg
     1321 gagccctcgt cccctgctcc caccaccatg gaaccatcac ggcccttcca gtgacggtgc
     1381 accgtgtagg cggcaagcgt cccctccacc aggctcacgg tcctgtcaag ttcggcgtgg
     1441 gacatcgaag cggagacgtc gagcttggag acgtccacgt tctcaggcgc cgagatgtcc
     1501 gaggagaggt tgccggtcct tagcctcagc ccgggcgtgt cccagaggac ctcgatggcg
     1561 ctcctgagcg ccgacgccct tgactccttg tt
//
LOCUS       DATAIR010000203         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_236597, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000203 DATAIR010000000
VERSION     DATAIR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236597"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VHM69_04865"
     CDS             complement(<1..516)
                     /locus_tag="VHM69_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2739757.1"
                     /translation="MSLKVRIIPCLDVDAGRVVKGTNFTNLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEQRDTAATLARRAAEEVFIPYTIGGGVRTADDMRAMLRAGADKVS
                     VNSAAVRNPDLITQGAERFGSQCVVLSVDVKRRSTSPGWEVYLNGGRVNTEMDAVSWL
                     VEGEKRGAGEFV"
     gene            complement(513..1235)
                     /locus_tag="VHM69_04870"
     CDS             complement(513..1235)
                     /locus_tag="VHM69_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2739758.1"
                     /translation="MLASSTVVPYPVTASYPERTRTAVKVVSLNVAQPSTQRYGREEV
                     RTGGNKRPVSKAVLRSRGFDGDGQADLVNHGGPEKAVCVYPFDHYAYWERVLGHRLQW
                     GAFSENLTVSGAVETEVCIGDVFKVGDAVAQVSQPRMPCGKLAGKNGQRLLTKWVGQT
                     GYTGFYMRVLKEGLVRSGDAFEIVENHHDFISIADVNGIIYGRSDDVGLIERLANLPE
                     FGADGRALFAERLVRGREKSIK"
     gene            complement(1242..2090)
                     /locus_tag="VHM69_04875"
     CDS             complement(1242..2090)
                     /locus_tag="VHM69_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX2739759.1"
                     /translation="MGCALAWRPGEARDKVSCLMVIDERGSIIANSFAASVEDIAGAI
                     EGYGTDRRGVLVGVDAPLAVPNERGTRKIERILSKVALPAYSASRRMFNNEPYSEELL
                     AELEKVGVEYTDYPFPRERGQRVVVEVDSAASLKVLSLERSGAADNGDLAQRLRAMND
                     PKFRKGNKESRASALRSAIEVLWDTPGLRLRTGNLSSDISAPENVDVSKLDVSASMSH
                     AELDRTVSLVEGTLAAYTVHRHWKGRDGSMVVGAGDEGSVLLPAKTALRERLAEECGT
                     ASVPYV"
BASE COUNT          336 a          760 c          646 g          409 t
ORIGIN      
        1 gacaaactcc ccggcccccc gcttctcgcc ctcgactagc cagcttaccg catccatctc
       61 tgtgttgacc cgccctccgt tcaggtagac ctcccagcct ggggaggtac tccggcgctt
      121 cacgtcaacg ctcaggacga cacactggct cccgaagcgt tccgctcctt gcgtgatgag
      181 atccgggttc ctcacggcgg cgctgttgac ggagactttg tcggcccccg cgcgcagcat
      241 ggcgcgcatg tcgtcggccg tcctgacgcc gcctcctatg gtgtatggga tgaagacctc
      301 ctcggcggcg cggcgggcga gcgtggcggc tgtgtccctc tgttcgtggg aggctgtgat
      361 gtcgtagaag acgatctcat cggcgccttc cctgtcgtag agcgccgcta gttccacggg
      421 atccccggcg tccctcaggt tcgtgaagtt ggtccccttc acgacgcgcc cggcgtccac
      481 gtcgaggcac gggatgatgc gaactttaag gctcacttta tgctcttctc ccgcccgcga
      541 accagccgct ccgcgaagag ggcgcgcccg tcggcgccga actcggggag gttggcgagg
      601 cgttcgatca accctacgtc atccgaccgc ccgtagatga tgccgttcac gtcggcgatg
      661 gagatgaagt catgatggtt ctcgacgatc tcgaaggcgt cgccggatct cacgagtccc
      721 tccttcaaaa cccgcatgta gaagcccgtg tagccggtct gtcccaccca cttcgtcagc
      781 agcctctggc cattcttacc ggcgagcttg ccacaaggca tacgtggctg gctgacctgg
      841 gccaccgcat ctccaacttt gaacacgtcg cctatgcaga cctccgtctc gacggcgccc
      901 gagacggtca ggttctcaga gaaggcgccc cattgcagcc ggtggccgag caccctctcc
      961 cagtacgcat agtggtcgaa ggggtagacg cacacggcct tctccgggcc accgtggttc
     1021 accagatcgg cctgcccgtc tccgtcgaaa ccacgggaac ggagcacggc cttagatacc
     1081 ggcctcttgt tcccgccagt cctcacctct tctcgcccgt agcgttgagt cgagggttgg
     1141 gccacgttca aagaaacgac cttcaccgcc gtcctggtcc tttcggggta gcttgccgtt
     1201 acagggtagg gtacgaccgt agaacttgcc agcacctggg gctacacgta gggtacgctg
     1261 gccgtgccgc actcttcggc caggcgctcg cgcagggcgg tcttcgcggg cagcaggacg
     1321 gagccttcgt cccccgcacc caccaccatg gagccgtccc ggcccttcca gtgacggtgc
     1381 accgtgtagg cggcgagcgt cccttccacc aggctcacgg tcctgtcaag ttcggcgtgt
     1441 gacatcgaag cggagacgtc gagcttggag acgtcgacgt tctcaggcgc cgagatgtcc
     1501 gaggagaggt tgccggtcct tagcctcagc ccgggcgtgt cccagaggac ctcgatggcg
     1561 ctcctgagcg ccgacgccct tgactccttg ttgcccttgc ggaacttggg gtcgttcatc
     1621 gcgcggagcc gttgggcgag gtcaccgttg tccgccgcgc ccgagcgttc gagggacagg
     1681 accttcagcg aagcggccga gtccacctcg acgaccactc tctgtcccct ctccctcggg
     1741 aaagggtagt ccgtatactc gacgccgacc ttctccagct ccgcgagcag ctcctccgag
     1801 tacggttcat tgttgaacat cctgcggctt gcggagtagg cggggagggc caccttggag
     1861 aggatgcgtt ctatcttgcg cgtccccctc tcgttgggga ccgcgagcgg cgcgtcgacg
     1921 ccgacgagga cgccgcgccg gtccgtgccg tagccctcga tggcccccgc tatgtcctcc
     1981 acgctcgccg cgaaggagtt ggcgatgatg ctcccccgct cgtctatcac catcaggcac
     2041 gagaccttgt cccgcgcctc gcccggcctc cacgccaggg cacagcccac aaacctcaag
     2101 ctcactcctc tccggccacg acccgcgaga cctcctcgca gactcggcgg c
//
LOCUS       DATAIR010000204         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_236604, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000204 DATAIR010000000
VERSION     DATAIR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236604"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VHM69_04880"
     CDS             <1..619
                     /locus_tag="VHM69_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2739760.1"
                     /translation="EAFGISDLADPANYMNSQVFGFAPLALSFFPIMVLAGSIAGAEE
                     RGTIDILLGNPMPRWQLVIGNFIATALSLLAICAIVGLLTWGTAVLIDVDLSLQQVTD
                     AVLNMWPISLLAGAVALLCSALFHRRGLAIAIPAFLLFGMYLLDTIGRASEDLEDLRP
                     ISVFYYYGSAIEKGIDWTNFGSITLVALVLALLAVLVFRRRDVYT"
     gene            734..2032
                     /locus_tag="VHM69_04885"
     CDS             734..2032
                     /locus_tag="VHM69_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2739761.1"
                     /translation="MDTVLLRVRGLAALRGVLDSPVARDFLALLELLRTGRPDPEALA
                     RVVGRLWEGLAVEDEYLLPDAWQSHIVSRVLDDENPFSLGAEKRETRPVVLEQAGRDL
                     RALREVFALDAATLIRRVESTVPDLAGVWVPWKNPEPAEETPRHTIARKLSAAGDWGA
                     CAELLLGHYARHGAGPFGHHRAFHWKEGRLRAISRPDPVRLAELVAYEREREPLIKNT
                     ERFLAGLPAHHALLYGLPGTGKSSTVKAVLNEYAHDGLRLVEVTKEDLSELPGVLETL
                     RGRGPRFVLFIDDLSFEEHEVEYKTLKALLEGSVEEPPENVRLYATSNRRNVVRENFS
                     DRGDAGDDVHVRDTMQEKLSLAARFGLRLTFPSPDQSRYLQIVAGLARERGLEVPAEE
                     LRERALLWDRWHAGRSGRTARQFVDELQAELAERADRLPG"
     gene            2294..>3162
                     /locus_tag="VHM69_04890"
     CDS             2294..>3162
                     /locus_tag="VHM69_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739762.1"
                     /translation="MKGEMMFAGSMQGSHRVLTLIVALVAAALTVALIVLPANRADAL
                     VAGSLTPTISSDQADYPPGATVTLTGSNWQPGESVHINVNDESGRTWSRDVDVTADES
                     GAIRDEFQLPNWFVATYKVTATGAQSGEATTSFTDAAVTVQGQSNPVCTSGGQCDGGY
                     QSGNLTGWKELDQVPFRLKFTSTGHYTVVVDFDHVLNSDSTKTGIQNLYDWAGGNGVT
                     LNSATLTDSTGPVWAYTVDVNVTSIPQPAGLAAVTFKGNLSAGAHNFTGSSLAIGADN
                     GGGNVQIVKPGAAP"
BASE COUNT          596 a          989 c          999 g          578 t
ORIGIN      
        1 ggaggcgttc ggtatatccg acctcgccga cccagccaac tacatgaact cgcaggtctt
       61 cgggttcgcg ccgctggcgc tatctttctt cccgataatg gtgctcgcag gctccatagc
      121 cggtgccgag gagcgcggca ccatcgacat ccttctcggc aaccccatgc cgcgctggca
      181 actggtcata ggtaacttca tcgccaccgc cctctcgctg ctggcgatct gcgccatagt
      241 gggattgctc acttggggca cggccgtgct catagacgtg gatctctccc tgcagcaggt
      301 gacggatgcc gtactaaaca tgtggcctat atctcttctg gccggcgctg tggcgctgct
      361 ttgctccgcc ctcttccaca ggcgggggct cgccatcgcc atcccggcct tcctgctctt
      421 cggtatgtac ctgctcgaca ccatcggccg ggcctccgag gacctcgaag acctgcgccc
      481 catctccgtc ttctactact acggttccgc catcgagaag ggcatagact ggacgaactt
      541 cggcagcatc accctcgtgg cgctcgtgct cgcgctcctg gctgtgcttg tcttccgccg
      601 gagggatgtt tatacatagc atctcagcac gctgaggctt cgccttcgct tgtcagcacg
      661 gcgcgaaggg cgcagcgtgc tgaaaggcgg cgtgctgcga tgctaaagtt gcttcgtcat
      721 ggagcgcggg gacatggata cggttctgct cagggtgcga ggactcgcgg cgttgcgcgg
      781 tgtcctggat tcgcctgtcg cgcgggactt cctggcgctc ctcgaactgc tccgcacggg
      841 gcgcccggat cccgaggctc tcgcgagggt cgtcggacgg ttgtgggagg ggctagctgt
      901 cgaggacgag tatctgcttc ccgacgcctg gcagtcacac attgtcagca gggttttgga
      961 cgatgagaac ccgttcagcc tgggggctga gaagcgcgag acgaggcccg tcgtgctcga
     1021 gcaggcgggg cgggatctgc gcgcgctgcg agaggtgttc gcgttggacg cggcgacgtt
     1081 gatccgtagg gtcgaatcaa ctgtgccgga tctggccggg gtctgggtac cctggaagaa
     1141 tcccgagccc gcggaggaaa ccccgcgaca cacgatcgcg cgcaagctct ctgcggccgg
     1201 agattgggga gcatgcgcgg agcttctttt gggtcactac gccaggcacg gcgcaggacc
     1261 tttcggccac cacagggctt tccactggaa agaaggacgg cttcgagcca tctcacgtcc
     1321 cgaccccgtg cgtctcgcgg aactcgtcgc ctacgagcgc gagcgggagc ccttgatcaa
     1381 gaacaccgag cgatttctcg ccgggctacc cgctcaccac gccctcctgt acggcctgcc
     1441 ggggacgggc aagtcttcca ccgtcaaagc cgtcctgaac gagtacgcgc acgacgggct
     1501 gcggctggtg gaggtgacga aggaggatct ttcggagttg cctggagtgc tagagaccct
     1561 gcgtgggcgt gggccgcgtt tcgtcctctt catcgacgac ctctccttcg aggagcacga
     1621 ggtcgagtac aagaccctca aggcgctcct cgaaggcagc gtcgaggagc cgcccgagaa
     1681 cgtcaggctc tacgcaacct ccaaccggag gaacgtggtc agggagaact tctccgaccg
     1741 tggcgacgca ggcgacgacg tgcacgtccg cgacaccatg caggagaagc tctccctggc
     1801 ggcccgcttc gggttgcgtc tgaccttccc ctcgcccgac cagagccggt acttgcagat
     1861 cgtagccggg cttgctcgcg aacgcggcct cgaggtccca gcggaagagt tgagggagcg
     1921 cgccctcctg tgggaccgct ggcacgccgg acgctcgggg cgcacagcca ggcagttcgt
     1981 ggacgagctc caggccgagc tggccgagag ggcggaccga ctcccagggt agcgctgagc
     2041 ggcggaatcc gatccggcgc ccgcagcgca ctgccgcgta gcccagataa gctagagagg
     2101 gcacgtattt cgaacgtttc tcgtctttcc gaacgtttcc tagctcaatt ggactagtca
     2161 tgtggactag tcatgtggac tagtaaaaga tcattgattt aagcgaggtt ggcgaaggct
     2221 ccgcgaggca aaatccctgc atcgtcctat atcaggtaat ccgtgggcac gggacacaaa
     2281 agtagcaagg tccttgaaag gggagatgat gttcgcagga tcgatgcagg gatcacaccg
     2341 agtacttact ctaatagtgg cgctcgtcgc ggccgcactc acggtcgcat tgatcgtgct
     2401 ccccgcgaac cgcgccgacg ccctggtagc agggtcactt acgccaacga tctcaagcga
     2461 ccaggcggac tacccacccg gagccacagt aactttgaca ggctccaact ggcagcctgg
     2521 cgagtcagtc cacataaacg tgaacgacga gtcgggcagg acctggagcc gcgacgttga
     2581 cgtaaccgca gacgaaagcg gcgctatccg cgacgagttc cagctcccca actggttcgt
     2641 cgccacctac aaggtaaccg caacaggcgc gcagtcgggt gaggccacta cctcattcac
     2701 tgacgcggcc gtaacggttc agggacagtc caatcctgta tgtacttcag gaggacagtg
     2761 cgacggaggc taccagagcg ggaacctgac aggctggaag gagctggatc aggttccctt
     2821 caggctgaag ttcacttcga cgggacacta tacggtcgtc gtcgatttcg accatgtcct
     2881 caactctgac agcacgaaga cgggcatcca gaacctctac gactgggcgg gcggaaatgg
     2941 tgtgacgttg aacagcgcca cgctgaccga cagcacggga ccggtgtggg cgtacacggt
     3001 tgatgtcaat gtcacgagca tcccgcagcc ggcaggcctg gcagcagtga ccttcaaggg
     3061 caacctctca gcaggtgctc ataacttcac aggcagcagc ctcgccatcg gcgccgacaa
     3121 cggcggcggc aacgtccaga tcgtgaagcc aggcgcagcg cc
//
LOCUS       DATAIR010000205         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_289865, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000205 DATAIR010000000
VERSION     DATAIR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289865"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VHM69_04895"
     CDS             <1..110
                     /locus_tag="VHM69_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX2739763.1"
                     /translation="VPVATVAVNGAANAGVLAAQILALSDPELAQRLEG"
     gene            114..1430
                     /gene="purB"
                     /locus_tag="VHM69_04900"
     CDS             114..1430
                     /gene="purB"
                     /locus_tag="VHM69_04900"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2739764.1"
                     /translation="MIERYTLPEIGAVWTEEAKYRAWLKVELAVCRARAQLGEIPMDE
                     VEELAEKADCTVERIHESEEETNHDVIAFVSTVAEAVDSPVSRHFHFGLTSSDVLDTA
                     GALQLRDALDLILAEAKNLTLLLTEMALEHRNTIMVGRTHGVHAEPTTLGHRFAVWAF
                     EMERNLDRLSHAGEVAAVGKISGAVGTYANVDPKVEAITCQELGLSSSPASTQVVQRD
                     RHAEVLAAIAILGSTMEKIALEIRGSQRTEVRELAEPFGRGQKGSSAMPHKRNPILAE
                     RLCGMARILRANASVGFENNALWQERDISHSSAERVVLPDSTILAFYMLRTTGRVLRG
                     LQVDKDRMLENLNSGGGIVFSGRVLLALVESGMGRDDAYQIVQGAAMRAWEGEGGFRE
                     LLEEDEEIRERLGDDLDDLFDPSYALRNLGVVFERVEELKERLEHD"
     gene            1423..>2108
                     /gene="purC"
                     /locus_tag="VHM69_04905"
     CDS             1423..>2108
                     /gene="purC"
                     /locus_tag="VHM69_04905"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2739765.1"
                     /translation="MTEELLYEGKAKKVFTTDDEGVLLHRYKDDATAFNAQKRGSWKD
                     KGKTNATMSAAIFAYLETHGVPTHFVEQIDDTAIKTKKLDMLPVEIVVRNVAAGSLSR
                     LLGFEEGRKLKAPIVELCYKDDELGDPLLNWYHFRELGVSDEDLEFCEELGLKVNEVL
                     APFFDERGIILVDFKIEAGRDADGDLMLADEISPDTCRFWDKETGEKLDKDRFRNDMG
                     GVEEAYAEMLR"
BASE COUNT          436 a          610 c          694 g          368 t
ORIGIN      
        1 gcgttccagt tgctaccgtc gccgtcaacg gcgccgccaa cgcaggagtc ttggctgccc
       61 agatcctggc tctctcagat cccgaactgg cgcagaggtt ggaagggtaa ggcttgatcg
      121 aacgctacac cctgccggag ataggggcgg tctggaccga agaggcgaag taccgcgcct
      181 ggctgaaggt ggagcttgcg gtttgtcgag caagggcgca actaggtgag atcccgatgg
      241 atgaagtcga agaactcgcc gagaaggcgg actgtaccgt cgagcgcatc cacgagagtg
      301 aggaagagac caaccacgac gtaatagcct ttgtctcgac cgtagccgaa gcagtggaca
      361 gccccgtctc tcgccacttc cacttcggac ttacttcctc agatgttctg gacactgcgg
      421 gggcgttgca gcttcgggac gcgctggatc tgatcctggc cgaggcgaag aacctgacgc
      481 tgttattgac agagatggcc ctggaacatc ggaatactat tatggtcggg cggacacacg
      541 gggtgcacgc ggagccgacg acgctggggc acaggttcgc tgtctgggcc ttcgagatgg
      601 agcggaacct ggacaggctc tcgcatgccg gggaggtcgc ggctgtcggc aagatctcgg
      661 gtgccgtcgg gacttacgcg aacgttgacc cgaaggtgga agccattacc tgccaggaac
      721 tagggctgtc ctcgtcgccc gcatcgacac aggtggtgca gcgcgacagg cacgccgagg
      781 tgctggccgc catagcgatc ctcggttcga cgatggagaa gatcgcgctc gagatcaggg
      841 gttcccagcg cacggaggtc cgcgaacttg ccgagccgtt cggccgcggg cagaagggct
      901 cctcagcgat gccccacaag aggaacccga tcctggccga gcgcctgtgc ggcatggccc
      961 gtatcctgcg cgcgaacgcc tctgtcggct tcgagaacaa cgcactgtgg caggagcggg
     1021 atatctccca ctcctcggca gagcgcgtcg tcctccccga ctcaacgatc ctggctttct
     1081 acatgctccg cacgacgggc cgcgtcctgc gcggtctcca ggtcgacaaa gaccgcatgc
     1141 tggaaaacct gaacagcggg ggcggcatcg tcttctcagg tcgcgtcctg ctcgcgctgg
     1201 tggagagcgg tatgggccgc gacgacgcgt accagatagt gcagggcgcg gcgatgcgtg
     1261 cctgggaggg agagggaggc ttccgagaat tgctggaaga ggatgaagaa atacgtgaac
     1321 ggttgggcga tgatcttgac gatctcttcg acccttcgta cgcgttgcgt aacctcggcg
     1381 tggtcttcga gcgtgtcgag gagctgaaag agaggttgga gcatgactga ggaactgctt
     1441 tacgagggca aggccaagaa ggtcttcacg acggacgacg aaggcgtgct actgcaccgc
     1501 tacaaggacg atgccacggc tttcaacgcc cagaagcgcg gatcgtggaa ggacaagggc
     1561 aagactaacg ccaccatgag cgctgccatc ttcgcctacc tggagacaca cggtgtccct
     1621 acccatttcg tcgagcagat cgacgatacg gccataaaga cgaagaagct ggacatgctg
     1681 ccggtcgaga tcgtggtccg caacgttgcc gctggctctc tcagcaggct gctcggcttc
     1741 gaggagggca ggaagctcaa ggctccgata gtcgagttgt gctacaagga cgacgagctc
     1801 ggcgatccgc tgctcaactg gtaccacttc agggagctcg gcgtctccga cgaggacctc
     1861 gagttctgcg aggagctcgg cctcaaggtc aacgaagtcc tcgcaccctt cttcgacgag
     1921 cgcggcatca tcctcgtcga cttcaagata gaggctggcc gggacgccga cggcgacctc
     1981 atgcttgcag atgagatcag ccctgatacc tgccgcttct gggacaagga gacaggcgag
     2041 aaactggaca aggaccgctt ccgcaatgac atgggcggcg tcgaagaggc ctacgccgag
     2101 atgcttcg
//
LOCUS       DATAIR010000206         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_51951, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000206 DATAIR010000000
VERSION     DATAIR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51951"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VHM69_04910"
     CDS             <1..294
                     /locus_tag="VHM69_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739766.1"
                     /translation="LVVYVTLGVLLAFRGGWHGVLAAIAMAFVATSVEWALYPFSYAW
                     ASIEDPAGYADTFGGVQRPDYAFWTTYDIIGVGISAALAQGLRTMAHVNPRDL"
     gene            complement(275..544)
                     /locus_tag="VHM69_04915"
     CDS             complement(275..544)
                     /locus_tag="VHM69_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739767.1"
                     /translation="MRTITIRYTAFRECPNCEGRGDDGASWRPTECKRCRGRGRLVTG
                     VEVEDSVEGVLQRSRYDFNALGEVYLLDFDEDEDLEVVRPRDPWG"
     gene            579..1004
                     /locus_tag="VHM69_04920"
     CDS             579..1004
                     /locus_tag="VHM69_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2739768.1"
                     /translation="MNYVVFLAVSAGMAIALQVTINSIGLKDLGIGGLIGVSGLATAV
                     VGFLLTISTQRPEYTGKALLCALASGVLGAFILGSIVLAAGHGGVARTLSLVIASQLI
                     AGLIVDRIGLLGPASQEIGAGKVLGIVFILIGGVLVVRS"
     gene            complement(1011..>1757)
                     /locus_tag="VHM69_04925"
     CDS             complement(1011..>1757)
                     /locus_tag="VHM69_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2739769.1"
                     /translation="PFPVLAANDARMKHLFDNRYGTGHSSIVALLSNTNLFLSGKAVV
                     VMGFGWVSRGLAKYADGMGARVIVCEPDPVKLLEAYSEGYQVMNSLQAAEVGDVFLTG
                     TGNLKVLGTEHFARMKDGALLANAGHYDHEFDLAALRETAARSREVRRNITEYELTDG
                     RRLHVLARGRLVNIAAADGHPVEIMDLTFAVQALAAHHLANHAAGIEPGIQPIPEEID
                     DLVARAKLASLGVEPETLTEEQISYQQSWR"
BASE COUNT          279 a          586 c          554 g          338 t
ORIGIN      
        1 cttgtcgtct acgtgacgct cggcgtcctg ctcgcgttcc gcggcggctg gcacggggta
       61 ctcgccgcca tcgcgatggc tttcgtggcc acctccgtgg agtgggcgct ctatcccttc
      121 tcgtacgcct gggcatccat agaggatccc gcaggctacg cggacacctt cggcggcgtg
      181 cagcgtcccg actacgcctt ctggaccacc tacgacatta tcggggtggg gatctccgca
      241 gccctcgccc agggactacg cacgatggcc cacgtcaacc ccagggatct ctaggccgca
      301 cgacctccag atcttcgtcc tcgtcgaagt cgaggagata tacctcgccg agggcgttga
      361 aatcgtagcg cgaacgctgc aggactccct ccacggagtc ctcgacctcg acgccggtca
      421 cgagcctgcc ccttcccctg caacgcttgc actccgtcgg gcgccaggaa gcgccgtcgt
      481 cgccccgccc ttcgcagttg gggcactccc ggaaggccgt gtaccgtatc gttatagttc
      541 tcatgcttcg gatactatca gcaaggtggg aggcagcctt gaactacgtc gtgtttctgg
      601 ccgtgtccgc ggggatggcc atagcgttgc aggtgacgat aaactccata ggactcaagg
      661 acctcggtat tgggggcctg atcggggtct ccggactcgc gaccgcagtg gtcggcttcc
      721 tgttgacgat ctccacgcaa cgcccggaat acacgggcaa ggcgctgcta tgcgccctcg
      781 cctcgggcgt gctcggggcg ttcatcctcg gctccatagt cctcgccgca ggtcacggcg
      841 gcgtcgcccg cacgctctcg ctggtcatcg caagccagct catcgccggg ctcatcgtcg
      901 acaggatcgg actgctgggt ccggcctctc aggagatcgg cgcgggcaaa gtcctcggta
      961 tagtcttcat cctcatcggc ggcgtcctcg tcgtcaggag ctaggagcat ctaccgccag
     1021 ctctgctggt aggagatctg ctcctcggtc agcgtctccg gctccacgcc tagtgaggcc
     1081 agcttggcgc gggcgacgag gtcgtctatc tcctcgggga taggctggat gcccggctct
     1141 atgccggcag cgtggttggc gaggtggtgc gccgcgagcg cctgcacggc gaaggtgagg
     1201 tccatgatct cgaccggatg gccgtccgcc gcggcgatgt tgacgagccg cccacgcgcc
     1261 agaacgtgca gacgccgccc gtcagtgagc tcgtactcgg taatgttgcg ccgcacctct
     1321 ctcgatcgcg ccgcagtctc ccgtagggca gccagatcga actcgtgatc gtagtgtccc
     1381 gcgttcgcca gaagggcgcc gtccttcatc ctggcgaagt gctcagttcc caggactttg
     1441 agattccccg tgcccgtcag gaacacgtcc cccacctcgg cggcctgtag cgagttcatc
     1501 acctggtagc cctcggagta cgcctccagg agcttcacgg ggtcgggctc gcacactatg
     1561 acacgggcgc ccatgccgtc ggcgtacttg gcgaggccgc ggctgaccca accgaagccc
     1621 atcaccacta ccgctttgcc cgagaggaag aggttggtat tcgagagaag ggcgacgatg
     1681 gatgagtgac ccgtaccgta gcggttgtcg aagagatgct tcatcctggc gtcgttggcg
     1741 gccaggacag ggaacgg
//
LOCUS       DATAIR010000207         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_381811, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000207 DATAIR010000000
VERSION     DATAIR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381811"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /gene="purD"
                     /locus_tag="VHM69_04930"
     CDS             complement(<1..1063)
                     /gene="purD"
                     /locus_tag="VHM69_04930"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2739770.1"
                     /translation="MKVLVVGSGGREHALVEALAASPLQPEMYAAPGNPGISRIAETV
                     DIPVDDLVSLRDYAAENGIDLTVVGPETPLIGGISEAFWEADLKVFGPSRAAARIEGS
                     KVFAKELMRHAGVPTASFEAFDREEAAVAYLRAHRDYPVVVKADGAAAGKGVTIAHSR
                     EEAEEAVRAAFGGKFGSAGERIVIEECLEGREASVFVVTDGQEILPFLPAQDYKRAYD
                     GDEGPNTGGMGAYSPIMWMEPATYTAILDDIIRPTIHQLALTGAPYTGLLYAGVMVTE
                     TGPKALEFNCRFGDPETQVILPRLGSDLLELMLAAGNEDLAGREVVWSADKAVCVVLA
                     SEGYPETSSSGDEISGLENI"
     gene            complement(1082..2368)
                     /locus_tag="VHM69_04935"
     CDS             complement(1082..2368)
                     /locus_tag="VHM69_04935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX2739771.1"
                     /translation="MTATVVLGLAWGDEGKGRVCDALASDVGYVSRYSGGNNAGHTVR
                     VGEEEFKLHLVPSGIVREGVVCTIGNGVVVNPEVLVEEVTALEERGLSVRDRLKVDGR
                     AHLIMPYHIALDSHREIALGEARIGTTNRGIGPTYEDKVARYGIRVQDIFDEGILKTK
                     LKAALREKNAIFENVYGEDHYEVADVASWLLSFKEFIEPMVADTGTLLRDALENRERV
                     LLEGGQATLLDNDHGTYPFVTSSNPTVGGAVVGSGIPARHLEYVVGVTKAYTTRVGDG
                     PMPTELFDETGETIRSVGREYGTTTGRARRVGWLDLPAIKWASSLNGITHVAITLLDV
                     LSAVDEINICIGYEIDGKAVEGYPMNQTDLHHAKPVYKTLPGWGVDITGCRMRGDLPE
                     AAQDFVAFIEAEVGAPVCMISVGPERDQAIVERIGI"
     gene            complement(2579..3310)
                     /locus_tag="VHM69_04940"
     CDS             complement(2579..3310)
                     /locus_tag="VHM69_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX2739772.1"
                     /translation="MDLECLILHEAHDEDRLARLRGRIEAEGEQRNPVIVSPHEDRYL
                     VLDGAHRVRALGELGLRFALVQIVEPPERAEGWGHLLNGVGREDLGSIEGIKASEEPG
                     NAALAEVETGSGTTFFLWTEEEGLSSRVDAMWDLQSLYPEGVVVRRVEPDGAASLSDG
                     EALIRYQPFTPEELAAIVDSGSVLPAGVTRFRVRERVLGVRYPLQRMKDGDARTRNAE
                     LREFVESRWHENRVRYYREPVVLFE"
     gene            complement(3530..3603)
                     /locus_tag="VHM69_04945"
     tRNA            complement(3530..3603)
                     /locus_tag="VHM69_04945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3567..3569),aa:Pro,seq:ggg)
     gene            3664..4044
                     /locus_tag="VHM69_04950"
     CDS             3664..4044
                     /locus_tag="VHM69_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX2739773.1"
                     /translation="MALAASYIRTDPEGGLPTRDEQISAIAAYAAQNDHDLVAHYEDL
                     AAPGLLLYHRPSLKEAINNIKEIEDWEVLVIADPRCISDTESALHEFVHKLSLYGNRL
                     ESPGRPWEEFLADMKSYRREMSRK"
     gene            4093..4464
                     /locus_tag="VHM69_04955"
     CDS             4093..4464
                     /locus_tag="VHM69_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739774.1"
                     /translation="MSFILTVIGLLLLAIALAGIAFGIFMATEPGTREAGKLCAIWWV
                     PGVAAASGVLMRDVVTFTVGALCFLVAGAVFVFAGGGPRKPRVRRSRGDLARGPDGNR
                     RLDSENTTKENKTQSSGKAAS"
     gene            4626..4904
                     /locus_tag="VHM69_04960"
     CDS             4626..4904
                     /locus_tag="VHM69_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739775.1"
                     /translation="MGLIRTILALVILLILAHVALVYLDVEQGANVLTNAVYGLGTLL
                     ESPAEFLLNTVTALQEYLDPNSFYVVAITAAVLYFILYLLLGVGRRSG"
BASE COUNT          963 a         1682 c         1565 g         1009 t
ORIGIN      
        1 ggatgttctc caggccggag atctcatccc cagaagatga agtctcgggg tagccttccg
       61 aggcgagtac cacgcaaacg gctttatcgg cggaccagac gacctcgcgc ccggcgaggt
      121 cttcgtttcc agcagccagc atcagctcca ggagatcgga gccgagtcgg ggcagtatca
      181 cctgcgtctc ggggtcgccg aagcggcagt tgaactccag agccttcggg cccgtctcgg
      241 tgaccatcac gccagcgtag aggaggcctg tgtacggtgc cccggtcagg gcaagctggt
      301 ggatggtcgg gcgtatgatg tcatccagta tcgccgtgta cgtggcgggc tccatccaca
      361 tgatgggcga gtatgctccc atgccccccg tgttcggccc ctcgtcgccg tcgtatgcgc
      421 gcttgtagtc ctgggccggg aggaagggca ggatctcttg cccgtcagtg actacgaaga
      481 ctgaagcttc ccgcccttct aaacactcct cgatgacgat tcgctctcca gcggacccga
      541 acttgccccc gaatgcggcc ctcaccgctt cctcggcctc ttcgcgggag tgggcgatgg
      601 taacgccttt ccccgcagcg gcgccgtcgg cctttacgac gacaggataa tcccggtgcg
      661 cccgcaggta ggcgaccgcg gcctcctcgc ggtcgaaggc ctcgaagctg gcagtcggga
      721 cgccggcgtg ccgcatcagt tccttggcga acaccttcga accctctatg cgggcggcgg
      781 cacgcgaagg tccgaagacc ttgagatccg cctcccagaa cgcctccgag atacccccga
      841 tcagtggcgt ctcgggacct acgaccgtga ggtcgatacc gttctccgcg gcgtagtccc
      901 gcaaggagac caggtcgtct acgggtatgt ccaccgtctc ggcgatgcgt gagatgcccg
      961 ggttgcctgg ggccgcgtac atctcgggtt gcagggggct ggcagccagg gcttcgacca
     1021 gcgcgtgctc gcgcccgccg ctcccgacta ccagcacctt cacgggctcg gcctcacgac
     1081 tttagattcc gatcctctcg actatcgcct gatcccgctc cggtccgacg ctgatcatgc
     1141 acacgggggc cccgacttcg gcctcgatga aggcgacgaa gtcctgcgcg gcctcgggga
     1201 gatctcccct catcctgcac cccgtgatgt ccacgcccca tcctggcaac gtcttgtaga
     1261 ccggcttcgc gtgatgcagg tcggtctggt tcatcgggta gccttcgacg gccttgccat
     1321 ctatctcgta gccgatgcag atgtttatct catcgaccgc cgagagtacg tcgagcagcg
     1381 tgatggcgac gtgtgtgatc ccatttagcg aagaggccca cttgatggcc ggaagatcga
     1441 gccaccctac cctcctggca cggcctgtgg tcgtgccgta ctcgcgcccc acgctcctta
     1501 tggtctcgcc cgtctcgtcg aagagctcgg tcggcatcgg cccgtcgccc acccgcgtcg
     1561 tgtacgcctt ggtgacgccg acgacgtact cgaggtgccg cgctgggatg cctgaaccca
     1621 cgaccgcgcc gccgaccgtc gggttcgacg aggtgacgaa aggataagtg ccatgatcgt
     1681 tgtccagcaa cgtggcctgt cccccttcga gcaggactcg ctccctgttc tccagggcat
     1741 ctcgcaacaa cgtccccgtg tcggcgacca tcggctcgat gaactccttg aaggagagta
     1801 gccaggaggc gacgtctgca acctcgtaat ggtcctcgcc gtagacgttc tcgaagatcg
     1861 cgttcttctc cctgagggcc gccttgagct tggtcttcag gatcccttcg tcgaagatat
     1921 cctggacgcg gatgccgtag cgggcgacct tgtcctcgta tgtcgggcct attccgcggt
     1981 tcgtcgtccc gatcctggct tctcccagcg cgatctcgcg gtgcgagtcg agcgcgatgt
     2041 ggtaaggcat gatcaggtgc gcccgcccgt ccaccttgag acggtccctg acgctcaggc
     2101 cccgttcctc cagggccgtg acctcctcga cgagtacttc ggggttgacg actaccccgt
     2161 ttccgatagt gcagacaacg ccttcgcgca cgatgcccga tggaacgagg tgcagcttga
     2221 actcctcctc gccgacacga acggtgtgcc ccgcgttgtt gccgccggag tatcgcgaga
     2281 cgtatcccac gtcggacgcg agtgcgtcgc acaccctgcc cttgccctca tccccccagg
     2341 ccaatccgag gacgaccgtg gccgtcaaag tctcacctcc gcggtgacgt gaatcgtttc
     2401 aagatggacg cggctacccg gaaaaggaag tagccctgat ccgggaccga aacattctgt
     2461 ccgccgatcg gggccgccgc tctctgccac ggccgcagcg tagcacatag atttaacgtt
     2521 ttgtatcgcc ctggcaatat tcaacgaatc ccgaaccata tatctcgcgg gcctgcgatt
     2581 actcgaacag cacgacgggt tctctgtaat atctgacgcg gttctcgtgc cagcggctct
     2641 caacgaactc cctgagctcg gcgttcctgg tgcgcgcgtc tccgtctttc atcctctgga
     2701 gtgggtagcg tacgccgagg acacgctccc gcacgcggaa gcgcgttacg ccggcaggca
     2761 gaacgctacc cgagtccact atcgcggcaa gctcttctgg ggtgaacggc tggtagcgga
     2821 tcagggcctc gccgtctgag aggctggcag cgccgtccgg ttccacgcgg cgcacgacga
     2881 cgccctcagg gtagagcgac tgcagatccc acatcgcgtc cacgcgggaa ctcagaccct
     2941 cctcttctgt ccagaggaag aaggtcgtgc ccgagccggt ctccacctcg gcaagagccg
     3001 cattgccggg ctcctcggac gctttgatcc cttcgatgct tcccaggtcc tcccttccga
     3061 ccccgttcag gaggtggccc cagccctccg ccctctcagg aggctcgacg atctggacga
     3121 gggcgaagcg caagccaagc tctccgaggg cgcgcaccct gtgggcgccg tcgaggacca
     3181 ggtagcggtc ctcgtgcggg gagactatta cgggattgcg ctgttctcct tcggcctcga
     3241 tgcgcccgcg cagacgggcc aggcggtctt cgtcgtgtgc ctcgtgcagg ataagacact
     3301 ccaggtccac caggcgaagg tctttaagag aggggatcac gaacctggag ctagcgggac
     3361 gatttcgtcc ttcaggcgtc tcccccgcgg cggccccaga ggaagtcgag cagaacgaac
     3421 ccgccgaaga agaagatcgc gatgatgagg aagaacccta tgatgcccat gagcaggtag
     3481 agtgtgtcca attctcgcct ttcaacggaa caggtctcgc cgcaaacagt cgggacggcc
     3541 ggatttgaac cggcgaccac tcggccccca gccgagtgcg ctaccagact gcgccacgtc
     3601 ccgacgcgcc ttacttgaga cctggatcat accagacagg ccccatgcta tcatgcgatt
     3661 tgcgtggcgc ttgcagcatc gtacataagg actgatcccg agggcgggct ccccacgagg
     3721 gatgagcaga tttccgctat cgcggcctac gccgcccaga acgatcacga tctcgtagcc
     3781 cactatgaag acctcgcggc ccctgggctt ctcctctacc atcgcccctc tctgaaagaa
     3841 gcgataaaca acataaagga gatcgaggac tgggaggtgc tggtgatagc cgatccccgt
     3901 tgtatctctg atactgagtc ggcgctgcac gagttcgtcc acaagctctc actgtacggc
     3961 aaccgactcg aatctcccgg gcgtccctgg gaggagttcc tcgccgatat gaagtcctac
     4021 aggcgagaga tgagccggaa atagtacctg catccgcgtg aaacgcgtga cctcgtccgg
     4081 tagtatttaa aagtgagctt cattctgact gtaatcgggc ttctattgct ggcgatagcc
     4141 cttgccggca tcgctttcgg catcttcatg gctacggagc caggaacccg tgaggcaggc
     4201 aagctctgtg ccatctggtg ggtgcccggg gtggccgccg cgagcggcgt tctgatgcgc
     4261 gatgtcgtga cgttcactgt cggggcactc tgctttctgg tcgcgggcgc cgtgttcgtg
     4321 ttcgcgggcg gcgggccgcg caagccgagg gtgaggagaa gtaggggcga cctggccagg
     4381 ggaccagatg gcaatagacg cctggactcc gagaacacga ccaaggagaa caagacccag
     4441 agttcgggca aggccgcctc ctaaaccact cccaaaagcg gcggggatat tgctatactg
     4501 cccgcggaga acggccctga ggaggctcaa ggggagatat ggcccggtaa gatgggaacc
     4561 gcgcaaatcc ctcctgaccg tcctgggcaa aacagcgttg gtcacggagg caaggagata
     4621 gacgcatggg tctcatccgc acgatactcg ccctggtcat cctgctgatt ctcgcccacg
     4681 tcgcgctcgt ctatttggac gtggaacagg gggccaacgt actgacgaac gccgtctacg
     4741 gcctcggtac actgctggag tcacccgccg aattcttgct caacacggtg acggcgcttc
     4801 aggaatacct cgaccccaac agcttctacg tcgtcgccat caccgccgcc gtgctctact
     4861 tcatccttta cttgctgctc ggtgtaggcc gccgcagcgg ctagaagtcc agggatcaat
     4921 agatgtccct tcggcctgac ccatagctcc actagaaacc gtaaggttgc ggcatgcttg
     4981 cacgcaacaa caggggatgc tctatacttc atgtcaatcg ttttgccgca agaacggagg
     5041 catggtggat gtggcaatgg aggcaaggaa ggcttactgg gtagatttct atccatggat
     5101 cggctggaga acctatgatc gagtttaaga acgtaagcaa gacctacgcc ggctcggaca
     5161 ggtccgtggt caacgacctc accttcacgg tacaaaacgg ggagatctgt gtccttgta
//
LOCUS       DATAIR010000208        11035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_51980, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000208 DATAIR010000000
VERSION     DATAIR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51980"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHM69_04965"
     CDS             <1..311
                     /locus_tag="VHM69_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2739776.1"
                     /translation="IHHSDQGVQYASAEYVERLERAGARISMTSVGNPYENAKAESFF
                     RTLKVEEVYLKDYRNFEQAEQNIAEFIEEVYNRKRLHSSLGYLPPVEFEAIHALKMGS
                     "
     gene            complement(430..1290)
                     /locus_tag="VHM69_04970"
     CDS             complement(430..1290)
                     /locus_tag="VHM69_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2739777.1"
                     /translation="MMRRAYTTDLSNVEWGLIEPHLPVPKAPGRPRLHPLREIFDAVF
                     YILRSGCAWRLLPHDFPPWKTVYHYFRAWRLDGTWERINAAIRQRLRVHLGRDPQPSA
                     GIVDSKSVKSTGVRGEERGYDAGKQIKGRKRHILVDTEGFLLKVKVHAANVFDRDGIK
                     PLLQDAKELFPRLSHLWLDAGYNGRGKGKDWVEKALGLTAEIVRHPPKPRYVWVKEGE
                     EPDWEKLKELLPKPGFKVLPRRWVVERTFSWTDQNRRMSKDYERVPETGEAFIYTAMI
                     RLMARRLARS"
     gene            complement(1622..2728)
                     /locus_tag="VHM69_04975"
     CDS             complement(1622..2728)
                     /locus_tag="VHM69_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739778.1"
                     /translation="MARAVTLGLIPAPDIPEKIAEELASELPDLLSSRVDEGVSWDVA
                     VVVDPLTGTDREAPEILDECREKMLSEGWDLAICLTDLPVYRGGKLVAADVSAGRGVA
                     GLSLPALGAMRLRPKARDLTLQLAGELYERTKEHAAGDPPARGSQPRGSVGPFQRVDP
                     PDEDMRAMDVDARFAAPGASGHLRLWSGMVLANRPWKMLPAFKGAIAAAFATGAYALV
                     ITTLWLLADSVGWARLLLLMVTAIVAMVAWIIVAHHLWERPEDPDQQKWAALYNGVSV
                     LTVTSAVLCAYAILFALILLAAWVFVPGDYFQTILKHPVGFGEYLMLSWLAASLATIA
                     GALGASLEDEETVRKASYGYRQRRRHENDDAETQ"
     gene            complement(2884..5556)
                     /locus_tag="VHM69_04980"
     CDS             complement(2884..5556)
                     /locus_tag="VHM69_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2739779.1"
                     /translation="MATRMCDVCGIRPAVVKVRRVVPGEGQRVEHLCEIHAAEAGVGR
                     STFGGSSPLGGGSLFDDFFDRFFEEEPRGGGVVPRRQAEQVDITQFFNDSTTELLQRA
                     AGQAVEWGNLDLSSEHLLYAALEDNVVRRVLEGVEADPEAIRAQLEEEADKGGRTDVT
                     PSLAPDAKRALLAAYEESQALGSSYIGPEHVLLALARDDESEAGELLGRFGLSHTRLR
                     GAVMRGVDTATGAAREPASATPTLDEYSRDLTQMAREGKLDPVIGRAEEVETTIEILS
                     RRTKNNPVLIGDPGVGKTAIVEGLAQRIVNDEVPETLVGRRVLALDLSGLVAGTQYRG
                     QFEERLKQVVDEASENPEGQILFMDELHTVVGAGAAEGSMDASNMLKPALARGELHVV
                     GATTVDEYRKNVEKDAALERRFQPVLVGEPSVDDTIDILRGLKDRYEAHHRVKITEEA
                     IVAAAELSDRYVTDRFLPDKAIDLMDQASARVRLRSKTKPRDTKELEEEVARLGREKD
                     QAVSAEDFVRAQELKGRVEGLRSELDGSRDGARRPVAEVTAEDIAEVVSRATGIPVSQ
                     LTQEERERLMRLEEQLHERVVGQDEAVTAVAEAIRRARAGLSDPNRPIGSFLFLGPTG
                     VGKTELARTLAEALFGDEAAMVRIDMSEFQERHTVSRLVGAPPGYVGYEEAGQLTESV
                     RRRPYSVLLLDEIEKAHPDVFNILLQILDDGRLTDSQGRTVDFKQTVIIMTSNMGAQR
                     IQAHARRDESFEELKDDMMQILKNTLRPEFVNRIDEVIVFRSLGKEQIGEIARLLLDR
                     TTRRLRAQGIEVEFTEGAVGLIGEEGFDPEFGARPLRRTIQRRVDNELASMVLSGSLD
                     PGDRVVVDAAGEAGGLTFEVVAGAAAAEPPGEGG"
     gene            complement(6012..6491)
                     /locus_tag="VHM69_04985"
     CDS             complement(6012..6491)
                     /locus_tag="VHM69_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2739780.1"
                     /translation="MSAIAEKAGVQRHTYYAHFPKLKDLYQACAAHNLERNPVPDTSR
                     WAEISDPEERLRVAFSEVYAYFSREEPMLSNVLRDAPQDPILQQTMAPFYQYWQTVRD
                     VIAEAFEASAERREALLGAIALALDFQTWRTLVRQQGLDQDRAVELMVGMVRCLMHS"
     gene            6891..7142
                     /locus_tag="VHM69_04990"
     CDS             6891..7142
                     /locus_tag="VHM69_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739781.1"
                     /translation="MADAQRHLVRITPERLLLLVDLKDDRTLSALDLDARISVGDDYE
                     ACRQWSLAFNDEWPEMRGIAYAARWGCVRTTNVALFVER"
     gene            7449..7521
                     /locus_tag="VHM69_04995"
     tRNA            7449..7521
                     /locus_tag="VHM69_04995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7504..7506,aa:Ser,seq:cga)
     gene            complement(7711..8109)
                     /locus_tag="VHM69_05000"
     CDS             complement(7711..8109)
                     /locus_tag="VHM69_05000"
                     /inference="COORDINATES: protein motif:HMM:NF022970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739782.1"
                     /translation="MEWFAVVEWFGRPEIETDEARERRIVMEIVVDAYDERERAIGWY
                     YVEGNLNFPFLARCAARRSISHLKVGDVVEVIGMAPVEECEHEMFVEMDWEGDELAIP
                     LSQLEVIEADDETREAVEDWLYWTKKGYQF"
     gene            8629..8946
                     /locus_tag="VHM69_05005"
     CDS             8629..8946
                     /locus_tag="VHM69_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2739783.1"
                     /translation="MSGRKSFSKPRAKIDADPERRARMDEKRKSHDVLDKLSELRRER
                     RMTRAELAEALGLSQPAISKLETAASRGSVSGSEMFLSTLAGYIEALGGRLELHATFP
                     GLP"
     gene            complement(9151..10059)
                     /locus_tag="VHM69_05010"
     CDS             complement(9151..10059)
                     /locus_tag="VHM69_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739784.1"
                     /translation="MPNSIYKTPEGEAEIRTLYDEALAELGLVYESLTVSTRLGDTHV
                     LALGPEDSPPAVFLPGGNALNPTCLKWFLPLAERHRLYALDIVGQPGRSAQERPSPKG
                     DGHAFWVEDVLDGIGLSEPAPLVGISYGAGIAIRTMGLAPERVSRAALVSPAAIATGP
                     FLRILVEIGFPTLLYRLRPTEERLMRAAMPLYTEPEDPAFTPAVRQLGAALRHLRLDA
                     DLPRTATEEELRSFRGPVAVFASEDDAFFPARAVLQRAREIFPHLALAEILEGCRHVP
                     SKAAFGRVNERILAFLADPDETSSGR"
     gene            10443..>11035
                     /gene="budA"
                     /locus_tag="VHM69_05015"
     CDS             10443..>11035
                     /gene="budA"
                     /locus_tag="VHM69_05015"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEX2739785.1"
                     /translation="MPIDTGLVGALHVEYLMKRDLIDGQPHHHSLFQTSTIETLLEGN
                     YDGDVSFAELGARGDFGLGTLNALDGEMIALDGSFYQIRSDGRAYEVDEMARTPFAVV
                     TFFEPGLSQTLDTPIDFDGLCGRVDELLKTTSCCAVRVDGQFEYVKTRSVPRQRKPYP
                     LLVDVVKDQPTFELRDVAGSLVGFRFPDYAEGLNVTG"
BASE COUNT         2043 a         3519 c         3352 g         2121 t
ORIGIN      
        1 tgatccacca ctcagatcag ggggtgcaat acgccagtgc agagtacgta gagcggcttg
       61 aacgagcggg agcccggatc agcatgacat ccgtgggcaa cccctatgag aacgccaagg
      121 ccgagagctt tttcaggacg ctgaaggtgg aggaggttta cctgaaggac taccgcaact
      181 tcgagcaagc agaacagaac atcgctgagt tcatagagga ggtctacaac cggaagcggt
      241 tacactccag cctcgggtat ctgccaccag tagaattcga ggccatacac gccctaaaga
      301 tggggagttg acttgctcac tggtccggaa aatggggttc acaccaacaa aatagttacg
      361 gcctcttcca agaggaaaag ctgtggggta agctcggatt gggccatctg agaaactgtc
      421 tgaaaagcct catgagcggg ccaaacgtct tgccatcaga cgtatcatcg ccgtgtagat
      481 aaacgcctcg cccgtctcgg gcactcgctc gtagtctttg ctcatcctgc ggttctgatc
      541 cgtccacgaa aatgtccgct ccaccaccca acgtctcggc agcaccttga agccgggctt
      601 gggcaacagt tccttgagtt tctcccagtc gggctcctcc ccttccttaa cccatacgta
      661 gcggggcttt ggtggatgtc gcactatctc cgccgtcaac cccagcgcct tctctaccca
      721 gtccttgcct ttgccccggc cgttgtagcc ggcatccagc cacaggtgcg acagtcgcgg
      781 gaacagctcc tttgcgtcct gcaacagcgg ctttatcccg tcacggtcga agacgttggc
      841 tgcgtgaacc ttcaccttga gcaaaaagcc ctccgtatca accaggatat ggcgttttct
      901 ccccttgatc tgcttgcctg cgtcgtagcc gcgctcctct cctctcacgc cggtgctctt
      961 caccgatttg gaatccacaa tacctgcgct gggttgagga tctcgcccga ggtggacccg
     1021 cagacgctgg cggatggccg cgttgatcct ttcccaggtg ccgtcgaggc gccaggcgcg
     1081 gaagtaatgg tagacggtct tccacggtgg gaagtcgtgg ggaagtagcc gccaggcgca
     1141 gccgctacgg agaatgtaaa agacagcgtc gaagatctcg cgcagcgggt ggagcctggg
     1201 acgtccggga gccttcggaa ctggcaggtg gggctcgatg agtccccatt cgacgttgga
     1261 gaggtcggta gtgtatgctc ttctcatcat gctcggtgtc ctaccgagct accgcctcag
     1321 aacccctttt cagacagttt ctgatgctcc tttggacggg gaactcggat ggccctcgtt
     1381 ttaccttaca aacctcgcaa cactcatcta gttggagcac agagagaccg ctgagaagat
     1441 tgcagcacct tttatagtcc gaaagatgga tctcataatg caccgagtca ccgaagagtg
     1501 tgggctcact gaaaaagtgc ggatggaagc atagcgaagg acacgagccg ttgcgcttta
     1561 ccgtgagggg accgccacgt cggccttgac aaacagtgcc ttctcatgga agttcgtgcg
     1621 gctactgtgt ctcggcgtcg tcgttttcgt ggcgtcgtcg ctgccggtag ccgtaggatg
     1681 ccttgcgcac cgtttcctcg tcttccaggc tggcccccaa ggctcccgcg atcgttgcca
     1741 gggaggccgc aagccaggag agcatcaggt actccccgaa gcccaccggg tgcttgagga
     1801 tggtctggaa gtagtcgcca ggcacgaaca cccacgccgc cagcaggatc agggcgaaca
     1861 agatggcgta ggcacacagc accgccgacg tgaccgtcag cacgctcacc ccgttgtaga
     1921 gcgctgccca cttttgttga tccgggtcct cggggcgctc ccacaggtgg tgggccacga
     1981 tgatccaagc gaccatcgcc acgatcgccg tgaccatcaa cagcagtaga cgcgcccaac
     2041 ccacggagtc ggccagcaac cagagggtgg tgatcaccag cgcgtaggcg cccgtagcga
     2101 aggccgcggc tatggctccc ttgaaggcgg gaagcatctt ccaggggcgg ttggcaagta
     2161 ccatccccga ccaaagcctg agatgcccgg acgcccctgg ggcggcgaag cgagcgtcga
     2221 cgtccatggc tctcatgtcc tcatcaggcg gatctaccct ctggaagggt ccgaccgacc
     2281 ccctaggctg cgaacctcga gcggggggat cgcctgctgc gtgctccttg gtcctctcat
     2341 acagctcgcc cgcgagctgg agagtgaggt cccgcgcctt cggacgcagc cgcatcgcgc
     2401 ccagggccgg aagcgagagg ccagccaccc cgcgccctgc gctgacgtcc gccgcgacga
     2461 gcttgccacc cctgtagacc ggcaggtccg tcaggcagat agcgaggtcc cagccctcgc
     2521 tcagcatctt ctcccggcac tcgtccagta tctccggcgc ttcccgatcg gtgccggtga
     2581 gggggtcgac cactaccgcc acgtcccacg agactccctc gtcgacccgg ctgctcagga
     2641 gatcgggcag ttcggaagcc agctcctcgg cgatcttctc ggggatgtcg ggggcgggta
     2701 tcagtcccag ggtcacagcg cgggccacga gcacctcctg gcagtgcctg tcgtaacgag
     2761 tactaccaca gtcctaccac tcgcgtagcg gggttaaacc gagggctcgg tcgtgcttca
     2821 cggttggcgt aaccccctat tcagcggagt acgtggacat tgaaaactgc tctagacagt
     2881 cgttcaccct ccctcgcccg ggggctccgc cgcggccgcc cccgcgacga cctcgaaggt
     2941 gagcccgccg gcttcgccag cagcgtccac aaccaccctg tcgccgggat ccaaggatcc
     3001 cgagaggacc atgctggcca gctcgttgtc tacccgccgc tgtatcgtac gcctcaaggg
     3061 cctcgccccg aactcggggt cgaagccctc ttcgcctatc agcccgaccg ccccttcagt
     3121 gaactcgacc tctatgcctt gggccctcag gcgcctcgtc gtccggtcga gcaggaggcg
     3181 agcgatctcg cctatctgct ccttgccgag ggagcggaag acgatgacct cgtcaatgcg
     3241 gttgacgaac tcgggacgca gggtgttttt gaggatctgc atcatgtcgt ccttgagctc
     3301 ctcgaaggac tcatcacgcc tggcgtgcgc ctgtatcctc tgtgcgccca tgttggaggt
     3361 catgatgatc accgtctgct tgaagtccac cgtccggccc tgcgagtccg tcaggcgtcc
     3421 gtcatccagg atctgcagga ggatgttgaa gacgtccggg tgagccttct caatctcgtc
     3481 caggagcaag actgagtagg gcctcctcct aacgctctcg gtgagctggc cggcctcctc
     3541 gtagcccacg taaccgggag gggccccaac caggcggctc acagtgtgcc gctcctggaa
     3601 ctcgctcatg tctatgcgca ccatcgccgc ctcgtccccg aagagggcct cggccagcgt
     3661 gcgggcaagc tcggtcttgc cgacgcccgt gggacccaag aacaggaagg agccgatcgg
     3721 gcggttgggg tccgagagtc cagcccttgc cctgcggata gcctcggcaa cggccgtgac
     3781 ggcctcgtcc tgccccacca ccctctcgtg cagttgctcc tcaaggcgca tgagccgctc
     3841 gcgctcctcc tgggtaagct gtgacaccgg gatgccggtg gcgcggctca ccacctccgc
     3901 tatgtcctcg gccgtcacct cggcgacggg gcggcgggcg ccgtcgcgcg acccgtccag
     3961 ctcggacctt aagccctcga cccggccctt gagctcctgg gcccgtacga agtcctcggc
     4021 cgagaccgcc tggtccttct ctcggcccag gcgcgccacc tcttcctcga gctccttcgt
     4081 gtcgcgtggc ttggtcttgg aacgcagcct gaccctcgcc gaagcctggt ccatgaggtc
     4141 tatggccttg tcggggagga agcggtccgt gacgtagcgg tccgagagct cggcagcggc
     4201 cacgatggcc tcctccgtta tcttcaccct gtggtgggcc tcgtagcggt ccttgaggcc
     4261 cctgaggatg tctatggtgt cgtccacgga gggctcaccc accaggaccg gctggaagcg
     4321 gcgctccagg gcggcgtcct tctctacgtt cttgcggtac tcgtcgacgg tggtcgcccc
     4381 gacgacgtgc agctcgcccc gtgccaaagc gggcttgagc atgttggagg cgtccatcga
     4441 gccttccgcc gcccccgcac ccaccactgt gtgcagctcg tccatgaaca gaatctgccc
     4501 ctcggggttc tcgctcgcct cgtctacgac ctgcttgaga cgctcctcga actggccgcg
     4561 atactgggtg cctgccacca gcccggagag gtcgagggcc aagaccctcc tgccgaccaa
     4621 ggtctccggc acctcgtcgt ttacgatcct ctgggcgagg ccctccacga tggctgtctt
     4681 gccgacgccg gggtccccga tgaggacggg gttgttcttg gtccgcctgg agaggatctc
     4741 gatggtggtc tctacttcct cggcccgccc gatgacggga tctagcttgc cctcacgggc
     4801 catctgggtt aggtcgcggc tgtactcgtc gagggtgggt gtggcgctgg ccggctcgcg
     4861 cgcagcgccc gtggcggtgt ctaccccgcg catcacggcc cccctgagcc tcgtgtgcga
     4921 gagtccgaaa cgacccaaca gttcgcccgc ctccgattcg tcatctctcg ccagcgcaag
     4981 caggacatgc tcaggaccta tgtaggagga gcccagagcc tgcgactcct cataggcggc
     5041 taagagcgcc cgcttggcat cgggggccag ggagggggtg acgtcggtgc gcccgccttt
     5101 gtcggcctcc tcctcgagct gggcccggat cgcttcgggg tcagcctcca ctccttcgag
     5161 cacgcgccgc accacattgt cttcgagcgc cgcataaagg aggtgctcgc tactcaggtc
     5221 taggttgccc cactcgacgg cttgcccggc cgcacgctgc aatagttccg tcgtggagtc
     5281 gttaaagaac tgggttatgt ccacctgttc tgcctgcctc cgggggacta ccccgccccc
     5341 gcggggttcc tcctcgaaga acctgtcgaa gaagtcatcg aaaagacttc cgccccccag
     5401 gggagagctg cccccaaagg tcgaacgccc gaccccggcc tccgcggcgt ggatctcgca
     5461 gaggtgctca accctctggc cttccccggg aacaacccgc ctcactttga cgactgccgg
     5521 cctgatcccg cagacgtcgc acatcctcgt ggccatcttc ttttgcctcc tactcaaggt
     5581 ttctgtccgg cgtggtggag gtcgtgctcc gtgcccgcgt aacggtccga tcggacgttt
     5641 cgtgacctac tctcgggtcg gcgctccgct ttaccccgcc cacccctcgt tacatacgtt
     5701 acccttttgc cggtcgccga aacgcgcttc ttcctcacgg aggggtattc acccaacgtc
     5761 gtggaagttt aattctcgga agtgagccgt agcaagcggc tcctctacgc accgccaagc
     5821 cattgacaat agaccctgcc gcccccctgt gtcgccgcgt ccgggcgact tggtagcagt
     5881 agttgcagca atgtgtatca aggggttgcg actcgtaacg atgcaactac ccaagaaatt
     5941 ctcgcccatc ggaccgggac tcaaccaaac tttgcctagg aacggagttc taagaaggtg
     6001 gtagagcgtc cctaggagtg catcaggcag cgcaccatcc ctaccatcaa ctccacagcc
     6061 cgatcttggt ccagaccctg ctgacgcacc agcgtgcgcc acgtctggaa gtcaagggcg
     6121 agggctatgg ccccgagcag cgcctcacgc cgctcggcgc ttgcctcgaa cgcctcagcg
     6181 atgacatccc ttaccgtctg ccagtactgg tagaagggtg ccatggtctg ttggagtatg
     6241 ggatcttgcg gtgcgtcacg cagcacgttg gacaacatcg gttcttcgcg agagaagtaa
     6301 gcgtagacct cagagaacgc taccctaagg cgctcctcgg gatcggagat ctccgcccaa
     6361 cgagatgtgt caggcacggg gttccgctcc aggttgtggg ccgcgcacgc ctggtagagg
     6421 tccttgagct tggggaagtg ggcgtagtag gtgtgcctct gcacccccgc cttctcggct
     6481 atggcgctca cggtggcccg tgccggtcac caaaagtact tagacagcat tccaagagct
     6541 aatggtagat agggtacctc ctggtgaaga gcacaaagct cgagagcatt atgaaaagct
     6601 atccgcgata gcgcgggctc gaacgttaaa cgtgtttgag gccttggacc cgatacgtaa
     6661 aggcaccatc atacgactct tggtctacgc tggctcgcta atggaccccg agcagaagaa
     6721 ggccgagccg gagaaacccg cgaagggtga tgcctcaggg acgatccgag tgtggttggg
     6781 cacggccgtc ccctcgcatc acaatttttc atcaggccgt cctttacgcc aacgacacca
     6841 aggtggccac cttcaacgag aggctacccc gagaagcgta ggcgcatccc atggcggacg
     6901 cgcagaggca cctggtgagg atcacgcctg agaggctgct tctactcgta gacctcaagg
     6961 acgaccgaac gctctcggct ctggatctcg acgcccgcat ctcggtcggg gacgactacg
     7021 aggcgtgccg ccagtggtcg ctagccttta acgatgagtg gccggagatg cgtggcatcg
     7081 cctacgcggc ccgctggggc tgcgtccgca ccacgaacgt cgccctgttc gtcgagcgct
     7141 gacgcgaaga cctcgtagtg gattccctcg gacgtctcag ggaccctgcc atcgaggatc
     7201 tagtgctaga agccgcggat cggtacaatc tgagggtcgc tttcctcacc taaagcgttg
     7261 ggcgtcctca ctcggcatta tgtgccggac ggggcatcag cgaagcgccc ctcggccgcg
     7321 ccccaaccgg gacacggcgt ggcagcagta gggcagcaat agggatgaca tgaggcggta
     7381 atcggtggta cacaattcgg ctcgtgggag gaaaatttcg gcttacctaa gccactttac
     7441 ggcacttggc ggcatccggc gacaaccgcc cttgacggac ttctaatccg ccggtcgcag
     7501 gttcgaatcc tgccgggcgc gcttctaaaa gccctgcaaa taacggaaaa ctaagagaaa
     7561 ctcgattatc ggttcttggc cctgtggcag cagtaggtgg aaaatttacc ccgattgaac
     7621 gggaaaatcg tggccgagca ccatcatctc cgactgccac agagcttgcc ccgatgaggt
     7681 gccatgagtg cccaggagcg cagcgttagc tcagaactgg tagcccttct tggtccagta
     7741 gagccagtct tcgaccgcct cccgcgtttc gtcatcggcc tcgatcactt cgagctggga
     7801 gagaggaatc gccagctcgt caccctccca gtccatctcg acgaacatct cgtgctcgca
     7861 ttcctccacg ggagccatgc cgatcacctc gaccacatct cctactttca ggtgggagat
     7921 tgatcttctg gcagcgcacc gggcgaggaa agggaagttc aaattccctt cgacgtagta
     7981 ccaccctatg gcccgttctc gctcatcgta agcatcgacg acgatctcca tgacgatccg
     8041 acgttctctg gcttcgtccg tttcgatctc aggtcttcca aaccactcga ccactgcaaa
     8101 ccactccaac cttgctggcc tttcacccca taccataagt atctgggctt ggatgcacaa
     8161 ccttcgaaac gtgggctcag gcgctcgacg acagggggcg cggtctcggt gcgggcgctc
     8221 gccccctttg caccgcccgt ggcagttggg aaccttattt cattatcgag ccacattctg
     8281 caataaggtg atcagatggc gccgcttatt tcattggcat gaggtaatga ggtagccagg
     8341 tattgtctgc tgcacgcttg cctcaaccat ttctggtcac cgttactctc ggttttcgcc
     8401 cgaaccaggt aagccccgtc tgcgccttgc tccattattt acccgcgggc tataccatcg
     8461 acggtgcagt gggaggtcga ccacaccgac gagttcgagg agtggtggga tgcgatctcc
     8521 gaggaggtgt acatagcact cgcgaccctg aaaagctcga ggagcagagg cagagcctcg
     8581 cgactacgac gagcgcctga acgagcttcg ggaggaagga gagataccgt gagtgggcga
     8641 aagagcttca gcaagccccg cgcaaaaatc gatgcggatc cagaacgccg ggcacgcatg
     8701 gacgagaaac ggaagtccca cgatgtcctc gacaagctct ccgagctgcg ccgggagcgc
     8761 aggatgactc gggccgagct agccgaggct ctaggcctta gccagccggc gatctccaag
     8821 ctcgagaccg ccgcctcccg ggggagcgtc tccggcagcg agatgttcct ctctacgctc
     8881 gctggataca tcgaggcgct cggcggccgc ttggagctac acgccacctt tccaggcctc
     8941 ccctaggacg agatcgccta cccgtgggag gaacctcgac ttcaccggat gcgaaggacg
     9001 cacaaaccta gacaaagtcc tggtggatca ccgtcaatcg gtgctgaggc tggaaggcca
     9061 tgttccacgg aggcccggat cgacgcggag tctgcaggga ccttgttccc cttcctcgtg
     9121 gtgtggtcgt gaggagcatc cagggcgcct ttagcgaccg ctcgaggttt cgtcgggatc
     9181 cgcgaggaag gcaaggatgc gctcgttcac acgcccgaac gccgccttcg aggggacgtg
     9241 gcggcagcct tcgaggatct cggctagggc gaggtggggg aagatctctc tcgccctttg
     9301 gaggaccgcc cgagctggaa agaacgcgtc atcctctgag gcgaacaccg caacaggccc
     9361 tctgaagctt ctgagttcct cctcggtggc cgtacgtgga agatcagcgt ccagccggag
     9421 gtgccggagg gccgctccta gctggcgtac ggcgggggtg aaagcgggat cctcgggctc
     9481 cgtgtacaga ggcatggccg cgcgcatgag tcgctcctcg gtgggacgga gccggtagag
     9541 gagcgtgggg aagcctatct cgacgagaat ccgcaagaag gggcccgtgg ctatcgcggc
     9601 gggggagaca agcgctgccc tagagacgcg ctccggggcg aggcccatgg tcctgatggc
     9661 tataccagcc ccgtaggaga tgccgacgag gggcgcgggt tcgctcaggc cgatgccgtc
     9721 aagcacgtcc tcaacccaga aggcgtgtcc gtctcccttc ggcgagggcc tctcctgagc
     9781 gctcctgccc ggctggccga cgatgtcgag tgcataaagg cggtggcgct ccgccagcgg
     9841 caggaaccat ttcaggcaag tggggttcag ggcgttcccg ccagggagga acaccgcggg
     9901 tggggaatcc tcggggccga gagcgaggac gtgcgtgtcg ccaaggcgcg tgctgacggt
     9961 caggctttcg tagacgaggc ccagctcggc cagcgcctcg tcgtagagcg tacgtatctc
    10021 agcctccccc tcgggcgttt tgtagatgga attgggcatg gtgtacctct cacgagagga
    10081 gccgacaagt cccgtcagtg tcgcaggctc cctcggccgc gtccatattg agccagatgg
    10141 acctccgacg gtggctgtcg caatccgaga tccactcttt cgacagcgtc gggctcgcta
    10201 tgctgcgcgc actttacggt aggttaggtg aggagcaggt catatgtcac gctaattctt
    10261 cctctggata gcagggtggc gagggcctgg ctaacaagat atcagggagc tggcgcggga
    10321 tgtgtgcgcc gtggatatgt catcaagatc ctggtcgtaa cgtcccgtcg acagctaccg
    10381 caggcggtgt tgcaacgcgt ctgctaaggt ccgaggcata acctactgaa ggaggggatc
    10441 ccatgccgat cgataccggg ttggtaggcg cgctgcacgt cgagtatctt atgaagcggg
    10501 atctcataga cggccagccc caccaccatt cgctgttcca gacttcgacc atcgaaactt
    10561 tgttagaggg caactacgac ggcgacgtca gcttcgcgga gctcggggcg cgtggagatt
    10621 tcggcctggg aactctgaac gcgctggacg gagagatgat cgccctcgat ggatctttct
    10681 accagataag gtcggacggg cgcgcctacg aggtcgacga gatggcgagg acgcccttcg
    10741 cggtggtgac cttcttcgag ccggggcttt ctcaaacgct cgacacgcct atagattttg
    10801 acggtctctg tggtcgcgta gacgagctcc tgaagacgac gagctgctgc gccgtcaggg
    10861 tggacgggca gttcgagtac gtcaagacac gcagcgtacc ccgccagcga aagccttatc
    10921 ctcttctcgt agacgtcgtc aaagaccagc cgaccttcga gcttcgcgat gtcgcgggaa
    10981 gcctcgtcgg cttccgcttc cccgactacg ccgagggtct gaacgtaacc ggcta
//
LOCUS       DATAIR010000209         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_105407, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000209 DATAIR010000000
VERSION     DATAIR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105407"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VHM69_05020"
     CDS             complement(<1..1128)
                     /locus_tag="VHM69_05020"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2739786.1"
                     /translation="MPGGSVSTELVIRHANVLDPVAGLDGIMDVRLSSGRISEVGDGL
                     RGSHELDADGLHLFPGFVDVHAHWRTPGREDEEDIESGSAAAAAGGFTGVVMMPNTDP
                     IVDRPVVVSGLMRRVERESRIRARVSAALHVGLAGERLTEMRLLKEAGAFCVSDDGLG
                     TASAGVLRNGMLYARSAGLPVILHCEDHTLATGVVHDGVAASLAGLPGSPASAEDAAT
                     ATALILAAETGAKVHITHVSTALSADLVGFFKDRADVTADTTPHHLTLTDDLVSTLEG
                     LYRVNPPLRPDDDRGGVEAALREDILDFVATDHAPHASEEKELPIEEAAPGFLGHETA
                     FGALYTDLVLEGRLSLARLVEAMSCAPGRWVGEGGNLAVDAP"
     gene            complement(1128..2045)
                     /locus_tag="VHM69_05025"
     CDS             complement(1128..2045)
                     /locus_tag="VHM69_05025"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2739787.1"
                     /translation="METRSFLTLEDATREDLRELLDLARACAQGHIRDILAGKSVCLA
                     FFEASTRTAVSFELAAKRSGADVISLSDKGSAIAKGESLIDTVVTLDRLGADAIVLRH
                     PAAGAAALASRHTSAPVINAGDGCGQHPTQALLDLYALSEALGGFEELAGPRLAIVGD
                     VLHSRVARSVIPGFLRAGMELALVAPRTLLPPDARVWGLPVLSSVDDAIDWGADVLYM
                     LRLQRERMTGARIPSVAEYARFYGVARRHLDKGVLVMHPGPVNRGVEISGDVVLDASS
                     LIPDQVAAGVCVRSAVLALSTGAAQKVAA"
BASE COUNT          371 a          684 c          683 g          358 t
ORIGIN      
        1 gggcgcatcg acggcaaggt ttcctccctc accgacccac ctgcccggtg cgcaactcat
       61 cgcttcgacc agacgcgcga gcgagagtct accttcgagg accagatccg tgtagagcgc
      121 cccgaaagcc gtctcgtggc cgaggaatcc aggcgcggcc tcttctatgg gcagttcctt
      181 ctcctccgag gcgtgcggcg cgtggtccgt cgcgacgaag tccagaatat cttctcgcag
      241 cgccgcctca actccgccgc ggtcatcgtc tggcctgagc ggcggattga cacggtagag
      301 cccttctaac gtggatacga gatcgtctgt cagcgttaaa tggtgcggcg tcgtgtctgc
      361 ggtcacgtcg gcccggtcct tgaagaatcc gacgagatcc gccgagagcg ccgtcgagac
      421 gtgggtgatg tgcaccttcg caccagtctc ggcggcgagg atcagagccg tcgccgtcgc
      481 cgcatcctcg gcgctcgcgg gggagcctgg aagtcctgcg agcgaggcgg ccacgccgtc
      541 gtgcacgacg cctgtggcga gcgtgtggtc ctcgcagtgc aggatcaccg gtagccccgc
      601 cgagcgggcg tacagcatgc cgttcctgag gacgccggcg gacgccgtcc ccaggccgtc
      661 gtcagagaca cagaaagctc ccgcttcctt caggagacgc atctcggtca ggcgttcacc
      721 cgccagcccg acgtggaggg ctgcggagac acgcgctctt atcctggatt cacgctctac
      781 tcggcgcatc aggccgctca ccacaacagg gcggtccact atgggatccg tgttcggcat
      841 catgactacg cctgtgaatc cgcccgctgc ggcggcggcg gagccgctct ctatatcctc
      901 ctcgtcctca cgtccaggtg tacgccagtg ggcgtgtacg tcgacgaagc cggggaagag
      961 gtgaaggccg tcggcgtcca gttcgtggga gccgcgcagt ccatcaccga cctccgagat
     1021 cctgcctgat gagaggcgta cgtccatgat cccatcgagg cctgcgaccg gatctagcac
     1081 gttggcgtga cgtatgacga gttcggtaga gacgctgcct cccggcatca agctgcgact
     1141 ttctgggctg cgcctgtcga gagggcgagg acggcagagc ggacacacac tcctgcggcg
     1201 acctggtcgg ggatcaggga ggaggcgtcg aggacgacgt cgcctgagat ctcaaccccc
     1261 ctgttgacgg gtcctggatg catgacgagc acacctttgt ccagatggcg ccgcgctacg
     1321 ccgtagaagc gggcgtactc tgcgaccgac ggtatccgag cacccgtcat cctctcccgc
     1381 tgcagccgca gcatgtagag aacgtcagcg ccccaatcaa ttgcgtcatc gacggaggag
     1441 agtactggca gtccccagac tcgggcgtcg ggcggcagca acgtgcgtgg ggccaccagg
     1501 gccagttcca tccctgcccg aagaaaaccc ggaatcacgc tgcgcgcaac gcggctatgt
     1561 aatacatcac cgacgatggc gaggcggggt cctgcgagct cttcgaaacc gccaagcgct
     1621 tcgctcaacg cgtagaggtc gaggagggcc tgagtagggt gctggccgca accgtctccg
     1681 gcgttgatca ccggcgcgga agtgtggcgg gaagcgaggg cggctgctcc ggcggccgga
     1741 tgccgaagca cgatcgcgtc agcacccagg cggtcgaggg tgacgacggt gtctatcaag
     1801 gactcgccct tggcgatggc cgagcctttg tcggagaggg agatcacatc agcccccgag
     1861 cgcttcgcgg cgagctcgaa ggagactgct gtgcgagtcg atgcctcgaa gaaggccaga
     1921 catacgctct tgcccgcgag tatgtctctg atgtggccct gtgcgcacgc gcgcgcgagg
     1981 tcgagcagtt cacgaaggtc ctcgcgggtc gcgtcttcga gggttaaaaa gctcctagtc
     2041 tccaacgacg atcaccccgt cctccccatc cgtctcctcc aggttgacaa gcaccc
//
LOCUS       DATAIR010000210         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_105465, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000210 DATAIR010000000
VERSION     DATAIR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105465"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHM69_05030"
     CDS             <1..190
                     /locus_tag="VHM69_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739788.1"
                     /translation="LDCGEQGVEAAKRAGGDGHADDGEVRPRGDRARAYAAVEAIHFP
                     GMQYRTDIALEAMELEDF"
     gene            190..612
                     /gene="purE"
                     /locus_tag="VHM69_05035"
     CDS             190..612
                     /gene="purE"
                     /locus_tag="VHM69_05035"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX2739789.1"
                     /translation="MPAVGILVGSKSDLPTMEKCTKRFEDLGVEHELEVLSAHRNPEA
                     VADYVAKAPDRGIKVFICAAGMAAHLAGAVAARTNLPVIGVPIAAGSLGGFDALLATV
                     QMPTGVPVATVAVNGAANAAVLAAQILALSDAELARRL"
     gene            618..1934
                     /gene="purB"
                     /locus_tag="VHM69_05040"
     CDS             618..1934
                     /gene="purB"
                     /locus_tag="VHM69_05040"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2739790.1"
                     /translation="MIERYTLPEIGAVWTEEAKFRAWLEVEIAVCRARARLGEIPMDE
                     VEELAEKVGFSVERIEEIEAETNHDVIAFVSNVAETVNSPVARHFHFGLTSSDVVDTA
                     GALRLRDALDLIREEAKDLARLLVEMSLEHRETVMVGRTHGVHAEPTVLGHKFAVWAF
                     EMERNLDRLEHAREVATVGKISGAVGVYANVDPKVEALACEELGIAPAQASTQVVQRD
                     RHAEVLSTLAIVGSTVEKIALEIRGSQRTEIRELSEPFGRGQKGSSAMPHKRNPILCE
                     RLCGMARLMRGYAQVGFEDNALWQERDISHSSAERVVLPDATIAALYMLRTVGRVLRG
                     LDVSRERMRENLNLGGGIVFSGRVLLALVEAGMSRDDAYRIVQGAAMRAWEGEGGFRD
                     LLSEDEEVRERLGSKLVGLFDPAYAVRNAGVIFERVEELKGRLERD"
     gene            1927..>2337
                     /locus_tag="VHM69_05045"
     CDS             1927..>2337
                     /locus_tag="VHM69_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2739791.1"
                     /translation="MTEALLYEGKAKKVFTTDEPDVLVHRYKDDATAFNAKKRGSWEG
                     KGRTNATMSAALFAYLEQNGVPTHFVEQVDDTSIKTKKLTMLPVEIVVRNLATGSIKR
                     LLGFEEGRRFKAPIIELCYKDDDLDDPLVNWYHLR"
BASE COUNT          462 a          678 c          803 g          394 t
ORIGIN      
        1 tctggactgt ggcgagcagg gcgtcgaagc cgccaagcga gccggcggcg atgggcacgc
       61 cgatgacggg gaggttcgtc ctcgcggcga ccgcgcccgc gcctacgccg ccgtcgaggc
      121 gatccacttc cccggaatgc agtaccgtac ggatatagcc ctggaagcca tggaactgga
      181 ggacttttag tgccagcagt aggaatactc gtcggcagca aatccgacct gcccacaatg
      241 gagaaatgca ccaaacgatt cgaagacctc ggcgtcgaac acgaactcga agtcctcagc
      301 gcccaccgca accccgaagc cgtcgcagac tacgtcgcca aagctccgga tcgcgggata
      361 aaagtcttca tctgcgccgc cgggatggcc gcccatctcg cgggggcagt agccgcgagg
      421 acgaaccttc cggtgatcgg cgtacccatc gccgccggat ccctgggcgg cttcgatgcc
      481 ctgctcgcca ccgtccagat gccgacgggt gttccggtcg caacggtcgc ggtaaacggc
      541 gctgccaacg ccgccgtgct cgccgcgcag atacttgctc tgtccgacgc cgagctcgca
      601 aggaggctct gagcagcttg atcgaacgct acaccctacc cgagatcggg gccgtctgga
      661 ccgaggaggc gaagttccgc gcctggcttg aggtggagat tgcagtgtgc cgcgcccggg
      721 ccaggctcgg tgagataccg atggacgagg tcgaagagct tgcggagaag gtcggtttct
      781 cagtggagcg tatcgaggag atcgaagcgg agaccaacca cgacgtgatc gccttcgtct
      841 ccaacgttgc ggagacggta aatagcccgg tagcccggca ttttcacttc ggcctgacga
      901 gttcggacgt cgtggacact gcgggcgcgc tgcgactgag ggacgccctg gatctcatcc
      961 gcgaggaagc gaaggatctc gcccggctgc tcgtcgaaat gtcgctggag caccgcgaga
     1021 cggtgatggt cggccggacg cacggggtac acgcggagcc tacggtcctc ggtcacaagt
     1081 tcgccgtgtg ggctttcgag atggaacgga acctggaccg gctggaacac gcgcgcgagg
     1141 tcgcgaccgt cgggaagatc tccggggccg tcggcgtata cgcgaacgtg gacccgaagg
     1201 tggaggcact ggcctgcgag gagcttggca tcgcaccagc gcaggcttcg acgcaggtcg
     1261 tgcagcgtga caggcacgcc gaggtgctct cgacgcttgc catcgttggt tcgacggtgg
     1321 agaagatcgc gctggagata agaggttcgc agcgaaccga gatccgggag ctgtccgagc
     1381 ctttcgggcg cggccagaaa ggctcatcgg cgatgccgca caagcggaac cccatcctgt
     1441 gcgaacgcct ctgcggcatg gcacgcctga tgcggggcta cgcgcaggtt ggcttcgagg
     1501 acaacgccct gtggcaggag cgggatatct cccactcctc ggctgaacgg gtcgtattgc
     1561 cggacgccac catcgccgcc ctctacatgc tccgtactgt gggccgcgta ctgcgcggcc
     1621 tggacgtgag ccgggagagg atgcgggaga acctgaacct tggtggaggc atagtgttct
     1681 ccgggcgcgt gttgctcgcg ctggtcgagg cggggatgag ccgggatgat gcgtacagga
     1741 tcgttcaggg tgcggccatg cgggcctggg agggcgaggg tggattcagg gatctgctgt
     1801 cggaagatga ggaagtacgc gaaaggcttg gttcgaagct ggtcgggcta ttcgatcccg
     1861 catacgcggt gcgaaacgcc ggtgtgatat tcgagcgagt ggaagaactg aaaggaaggt
     1921 tggaacgtga ctgaggcgct attgtacgaa ggcaaggcga agaaggtatt taccaccgat
     1981 gagccggatg ttctggttca tcgctacaag gacgacgcga cggcgttcaa cgccaagaag
     2041 cgcggttcgt gggaggggaa gggccggacc aacgccacga tgagcgcggc gctctttgcg
     2101 tacctggagc agaacggcgt gcctacccat ttcgtagagc aggtggacga cacgtccatc
     2161 aagaccaaga agctcacgat gcttccagtc gagatcgtag ttcgtaacct cgccacgggt
     2221 tcaataaaac ggctgctcgg gttcgaggaa gggcgacggt tcaaggctcc cattatcgag
     2281 ttgtgctaca aggacgacga cctcgacgat ccgctcgtca actggtatca cctgcgc
//
LOCUS       DATAIR010000211         8797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_144857, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000211 DATAIR010000000
VERSION     DATAIR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144857"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VHM69_05050"
     CDS             <1..609
                     /locus_tag="VHM69_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2739792.1"
                     /translation="LMLYAATGRTAYVLLGGVLFSLGSFVAFLLFEHVQLRVQSWIDP
                     WRDPEGTGFQILQSIFNIADGGLLGTGLGAGFAQTIPEVETDFVFSAIASELGLLGAT
                     AVLLAFLAFTYRGIKIALLAEDDASKLLAYGLTAMFALQTLIIVGGVTKTIPLTGITL
                     PFVSYGGSSVVGNFILTGLLLLVSERAGRREAEGLVYRDDPW"
     gene            615..2054
                     /locus_tag="VHM69_05055"
     CDS             615..2054
                     /locus_tag="VHM69_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2739793.1"
                     /translation="MNTHLRRTFYLFVAGFAGIVMMLVYWQVYSREALANNPENGFQT
                     QRSISSPRGLILAGDGETVLAKSVKHNTPAGPIYDRIYPLGEPFSNVVGYWSTRYGAT
                     GIEIGYNSDLSSIAGEPQTLDDLINRATGGPQPGNNVVLTLDPELQQLAYQELRESTT
                     GRGSVVAVDPKTGEILAMASSPSFDPNNIDENFPELAQNTGDPLLNRATQSLYPPGSV
                     FKVITAAAALEAGVSPEDTFFDDGSYELPGYTVHNFQGKDYGEVTFTKALAYSINVIF
                     AKIAVNIVGAEALTQMALDFGFADPYDDFSIPVSGSSVNSLPPEQWTTGTLAQTAFGQ
                     GEVQSNSFQMALVAAAIANDGKLMEPRLVREVRSPDGIILDKPTASVRERAIPAGTAI
                     TLNEMMQQVIIQGGLTEAEIPGVKVAGKTGTAESGNGLPHSWWITFAPADDPKIAMCV
                     MIENGGRADEGALPIAAPLMRAYLEGGSE"
     gene            2086..4155
                     /gene="pknB"
                     /locus_tag="VHM69_05060"
     CDS             2086..4155
                     /gene="pknB"
                     /locus_tag="VHM69_05060"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX2739794.1"
                     /translation="MQQLVDNRYRLLRPLGSGGMADVYLAHDAILDRDVALKVMSTRY
                     ASDEEFVERFKREAQSAAALSHPNIVSIFDRGESEDGTYYIAMEYLPGGTLKDRILKR
                     GALPARTAAAVALQMAEALRAAHDRDVIHRDIKPHNILITGSGDVKVTDFGIARAASS
                     STMTRTGHILGTAHYISPEQAMGEHVGPASDLYSLGVVLYEMLTGELPFDADTPLGIA
                     MKHVNGHARPPKELDPSIPDGINAITLRLLAKNPEDRYPNDSELIEDLERVSAGLDPA
                     GATTEMMTRAMPVAATARMNSPLPPQRVSRTKKRRGTAPLLIALLALLILAPLAWIGY
                     GLIQDRQDAQTLPRIGVPDLVDMTLDEARNEVGDDFDIQVTDSVENEKPVDTILSQDP
                     STGKAEKGSTITVTVVGTQVAGVPNVVGSERAAAEQTLKDAGFEVQVDEKESTFDDQG
                     LVMSQDPGSGARVEVGSGVTITVGTGPSSVEVPELYGNTPAQAESILQQTGLKLGART
                     QDYSNDVAEGSIFFQDPSEGENVEPGTAVDVTVSLGPEQVKIPEVYGLSLAAAQTTLS
                     NAGLNSTPVEVEGDEAAGTALSTDPGVGALLDPGSTVTLYYSAGPPPETTASPDASAS
                     QDPKKEEEKSDEGGNSESNNRPDGTNSGPGASGPGNGRPGRGDLLENVRENAPAAKKD
                     KKGKRKN"
     gene            complement(4160..4423)
                     /locus_tag="VHM69_05065"
     CDS             complement(4160..4423)
                     /locus_tag="VHM69_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739795.1"
                     /translation="MERAAADETVRIRAHSPGKYPILVVELPSGGLRAIYFETDYDLA
                     RAKPVEEDWLRENALGRHSFVEVNPPVETAAASLADYVRRELL"
     gene            4513..7005
                     /locus_tag="VHM69_05070"
     CDS             4513..7005
                     /locus_tag="VHM69_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2739796.1"
                     /translation="MTFIFVEGVTMSDPIPKDEGTEAGASEGTTDESLLNRLPPDGLP
                     EELLLRRLGRLDTRRALAELCRNVPGLEEILLPRGIQADALTRKSGGGAPLLRGIARR
                     VSQDAAAWGAFRTAVQEQIPPETFEALEGFSQENLEELTRAHTTESLLLAALSAEEEP
                     ADGVVDTLIEAWREEKGEAEKRASEDARVRHLESELERLKQDNERLSFASRAARERAA
                     ALSEEVEVLTGERTDTSELVRKAEERAASALDLRSQLDERIEKLEKRGAQLEKALEGE
                     RTAYSRAAERVDELHEELGRVVDERDRIKNALQDARFTDRGFGELLVRAIKNEVAALP
                     ASLESTAQAARLLEFMGKVLQAHADLRSPGEPRTVEEDPDDSDPSPASRPEETNGPRG
                     RSTFRRANGYARPRPTLSFRALGGAGEVGGSSHLLDFGNTRILVDAGIKPDGRGPLTP
                     DFGGLDGIDAAIITHAHLDHCGALPVLVRDRPDMPIYCTPPSAKLIVSALNDHAAMGG
                     GLPGGAPIHEVRKRLVPVPFGKPLDVGDAKVTLTESGHILGAASVLFTSGPATVFHTG
                     DICMENHFSIPSARLPEVKDIDLLIMEATLADQKPQPFSESIRTMVEVINDTTLERGG
                     TVLIPTYALGQAQEIILGIKHYSKEYGLDRDVFIYVDGSVVTTSERLYAEQLGYMKPY
                     LQHSDPREIFFSENIRAVANDDRARERILSNPCAIIASPVTMQGGASAFYRKRLEKHS
                     KNAVILPSNAASSYGTHRAEGEEEQWRVERVSFAAHCTQDELLGITEKLSPRQIILIH
                     GSRRRISDLAFRLAPNHKIHTPAVGETVRTVL"
     gene            7029..7337
                     /locus_tag="VHM69_05075"
     CDS             7029..7337
                     /locus_tag="VHM69_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739797.1"
                     /translation="MRLLDTRGERPANAAGIGEFWYEPEVWALPLSTDARVLYAGLCS
                     FLGHREINRKDLRNTLKGHPDSEISKALEELVRHDLLVPADNGTFRGYEVRPVGDFSV
                     "
     gene            7384..8301
                     /gene="coaA"
                     /locus_tag="VHM69_05080"
     CDS             7384..8301
                     /gene="coaA"
                     /locus_tag="VHM69_05080"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX2739798.1"
                     /translation="MHFSREEWSRLRDNTPLTLREEDLASLRGRNEKVSLEEVVEVYL
                     PLSRLLNLHITAAQGLYSVTDSFLGNPAAKVPYVLGIAGSVAVGKSTTARILQSLLAN
                     RPDHPEVALITTDGFLYPNHVLEERGLMKRKGFPESYDLRRLVRFVADVKAGHPEVSA
                     PVYSHLAYDILPGEMQYVRQPDLLIVEGLNVLQTGNGDPRNAPTAFVSDFFDFSIYVD
                     AEESHVERWFSERFLTLRDTVFKDESSYFHRYAHLSTEEARRVAAGIWADINGVNLRE
                     NIVPTRERAQLILQKGEDHQVLGVSLRKL"
     gene            complement(8307..>8797)
                     /locus_tag="VHM69_05085"
     CDS             complement(8307..>8797)
                     /locus_tag="VHM69_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739799.1"
                     /translation="YTAVLGRQAGFEIETTYAGDALDLRVVAGSEAGVSFGVVPGAAA
                     ETDENLMHEELSRYSLEPSGYSGRVLAWSQEAGDEPCQVFAVYSDGVWKTRGLFVAKA
                     RGRVDDPDDVLNGFCLRILGRLIDLASLTGGAGRRWADEPYTLEDLLDGRRSPTELRW
                     PG"
BASE COUNT         1722 a         2904 c         2756 g         1415 t
ORIGIN      
        1 ctgatgctct acgccgcgac gggccgcacg gcctacgtgc tgctcggggg agtacttttc
       61 tccttgggct ccttcgtggc cttcctcctc ttcgagcacg ttcagctcag agtgcagagc
      121 tggatcgacc cgtggaggga ccctgaaggg acggggttcc agatcctcca gagcatcttc
      181 aacatcgctg acggcgggct gctcggtacc ggtcttgggg ctggcttcgc ccagaccatc
      241 ccggaggtgg agaccgactt cgtcttctcc gcgatagcaa gcgagctcgg cctgctcggg
      301 gcgacggcgg tcttgctcgc atttctggcc ttcacctaca gggggatcaa gatcgccctg
      361 ctcgcggagg atgacgcctc caaactcctg gcctacgggc tcacggcgat gttcgccctc
      421 cagaccctca ttatcgtggg cggggtaacc aagaccatac cgctgacggg gatcacgctg
      481 cccttcgtct cctacggcgg ctcctccgtc gtcggaaact tcatactcac gggcctcctc
      541 ctgctcgtct cggagagggc gggccggcgc gaggccgagg ggctcgttta cagggatgat
      601 ccttggtgag gcccatgaac acccacctca ggcgtacctt ctacctgttc gtggcggggt
      661 tcgcaggtat tgtaatgatg ctcgtctact ggcaggtcta ctccagggag gcgctggcca
      721 acaaccccga gaacgggttt cagacccagc gcagcatctc ctcgccgcgg ggtttgatcc
      781 tggccggcga cggcgagacc gtgctcgcga agagcgtcaa acacaatacc cctgcaggtc
      841 ctatttacga tcgaatctat ccgcttggag agcctttctc caacgtggtc ggctactgga
      901 gcacccgcta cggggccact ggaatcgaga tcggctataa ctccgacctc tcgagcattg
      961 caggggagcc gcagaccctg gacgacctca taaatcgggc aacgggcgga cctcagccgg
     1021 gtaacaacgt cgtcctcacc ctcgatccgg agctccagca actcgcctat caggagctac
     1081 gcgaaagcac gacgggcagg ggctccgtcg tcgccgtgga cccgaagact ggggagatcc
     1141 tggcgatggc ctcctcccca tccttcgacc ctaacaacat agacgagaac ttcccggagc
     1201 tcgcccagaa caccggcgac cctctactca accgcgctac ccagagcctc tatcctcctg
     1261 gctccgtctt caaggtgata accgccgccg cggcgctcga ggccggggtc agcccggagg
     1321 acaccttctt cgacgacggc tcatacgagc ttcctggcta caccgtccac aacttccagg
     1381 gcaaggacta cggggaggtg accttcacga aggcgctggc ctactctatc aacgtgatct
     1441 tcgcgaagat cgcggtcaac atagtgggtg cggaagccct gacgcagatg gcactagact
     1501 tcggcttcgc cgacccttac gatgacttct cgatacccgt ttcagggagc agcgtcaaca
     1561 gccttccgcc cgagcagtgg accacgggca ccctcgcgca gacggctttc ggccagggcg
     1621 aggtacagtc caattccttc cagatggcgc tggtcgccgc ggccatcgcc aacgacggca
     1681 agctcatgga accccgcctc gtgcgcgagg tccgctctcc cgacggcata atcctcgaca
     1741 agccgacggc gagcgtgcgc gagcgggcga tcccagccgg caccgccatc acgctgaacg
     1801 agatgatgca gcaggtcatc atccagggcg ggctgaccga ggccgagata cccggcgtga
     1861 aggtcgcagg gaagacaggg accgccgagt cggggaacgg gctaccgcat tcgtggtgga
     1921 tcaccttcgc ccccgccgac gacccgaaga tcgcgatgtg cgtgatgatc gagaacggcg
     1981 gcagggccga cgagggcgcc ctgccaatag cggcccccct gatgagagcc tacctcgagg
     2041 gtggctctga atgatgaccg ccaccccgtt tataatattg agttagtgca gcagctcgta
     2101 gacaatcgtt acaggctcct gaggccgctc ggcagcggcg gtatggccga cgtatatctg
     2161 gcccatgacg cgattctgga cagggacgtg gccctgaagg tgatgagcac gcgctacgcg
     2221 agcgacgagg agttcgtcga gcgcttcaag cgcgaggccc agagcgccgc agccctctcc
     2281 caccccaaca tcgtctcgat cttcgacagg ggcgagtccg aggacgggac gtactacatc
     2341 gcgatggagt atctgccggg cggcaccctg aaggaccgca tcctgaaacg cggggcgctc
     2401 cccgcccgca cggcggcggc cgtggcgctc cagatggctg aggccctcag ggccgcgcac
     2461 gatcgcgacg tgatccaccg cgacataaag ccgcacaaca tcctcattac agggtcgggg
     2521 gacgtgaagg tgacggactt cggcatagcc cgcgcagcct cgtcctcgac gatgaccagg
     2581 accggccaca tccttggtac ggcccattac atctcccccg agcaggccat gggcgagcat
     2641 gtcggtccag cgagcgacct ctactctctt ggcgtcgtcc tctacgagat gctcacgggc
     2701 gagctgccct tcgacgccga tacgcctttg gggatcgcga tgaagcacgt caacgggcac
     2761 gcgcgcccgc cgaaggagct tgacccttcg atccctgacg gcataaacgc cataacgctc
     2821 aggttgctcg ccaagaaccc ggaagaccgc tacccgaacg actccgagct catagaagac
     2881 ctcgagcgcg tctcagcggg ccttgatccg gcgggcgcca ccacggagat gatgacccgg
     2941 gcgatgcctg ttgcggcgac cgcgcgcatg aactcccctc ttcctccgca gagggtgagc
     3001 cggaccaaga agcgcagggg gactgcgccg ctcctgatcg cgcttctggc gctcctgatc
     3061 ctggccccgc tcgcctggat aggctatggc ctgatccagg accggcagga tgcccagacc
     3121 ttgccgcgga tcggcgtccc cgaccttgtc gatatgaccc tggatgaggc cagaaacgag
     3181 gtcggtgacg acttcgacat acaggtaacc gacagcgtcg aaaacgagaa acccgtggat
     3241 actattctga gccaggaccc gagcaccggg aaggctgaga agggctcgac gatcacggtc
     3301 accgtcgtcg ggacgcaggt ggccggggtg cccaacgtcg tcggctcgga acgcgcggcg
     3361 gcggagcaga cgctgaaaga cgccggcttc gaggtccagg tggacgagaa ggagagcact
     3421 ttcgacgacc agggactggt catgagccag gacccaggga gcggggcgcg ggtggaagta
     3481 ggctcagggg tgaccatcac cgtcggtacg ggcccttcga gcgtggaggt tccagagctc
     3541 tacggcaata ccccggcgca ggctgagtcg atacttcagc agaccggcct gaagctcggc
     3601 gcccggaccc aggactacag caacgacgtc gcggagggca gcatcttctt ccaggatccc
     3661 tcggagggtg agaacgtcga gccggggacc gccgtcgacg tcacggtcag cctgggtccc
     3721 gagcaggtca agatccctga ggtctacggc ctgagcctcg cggcggcgca gacgaccctc
     3781 tccaacgcag ggttgaactc aacccccgtc gaggtggagg gcgacgaggc tgcgggcacc
     3841 gcgctctcga cggacccagg ggtcggagcg ctgctggatc caggctccac agtgaccctc
     3901 tactacagcg ccggtcctcc tcctgagacc acggcctctc cagacgcctc ggcctctcaa
     3961 gatccgaaga aggaggagga gaagtccgac gaaggcggca acagcgagtc taacaacaga
     4021 cctgacggta ccaacagcgg tcccggcgcc agcggacctg gcaacggccg acctggcagg
     4081 ggcgacctcc tggagaacgt aagggagaac gcgcccgcgg ccaagaagga caagaaaggc
     4141 aagcgaaaga actagggtct cagagtagct cacgcctcac gtagtctgcg agggacgctg
     4201 cggcggtctc tacgggaggg ttcacctcga cgaagctgtg gcggcccaag gcgttctccc
     4261 gcagccagtc ctcctcgacc ggcttcgctc tggcgaggtc gtagtccgtc tcgaagtaga
     4321 tggcgcgtag cccgcccgag ggcaactcga ctacgaggat ggggtacttt ccgggagaat
     4381 gagcccggat cctgaccgtt tcgtccgccg ctgcgcgctc cataccggga gtttatacga
     4441 gcgccgcagg caagagaagg caccgggagt aaagaagtgc cgtgatagaa ttggggagta
     4501 tgaagacgct ctatgacatt catattcgtg gagggagtca ccatgtcaga tcccataccc
     4561 aaagacgaag gaactgaggc cggagcctca gagggaacca cggatgaatc cctgctcaac
     4621 aggctacctc ccgatggact gcctgaggaa ttgctcctgc ggcgtctcgg gaggctcgac
     4681 accaggcgcg cgctcgcgga gctctgcagg aacgtgccag gcctcgaaga gatactcctt
     4741 ccaaggggca tccaggccga cgccctgacc cgcaagtcag gcggcggcgc gccgctcctc
     4801 agggggatcg cgcgcagggt ctcccaggac gccgccgcct ggggcgcgtt ccgcaccgcc
     4861 gtccaggaac agatcccccc cgagaccttc gaggccctcg aaggtttctc ccaggagaac
     4921 ctcgaggagc tcacacgggc ccacaccaca gagagccttc tgctcgcggc cctgagcgcg
     4981 gaggaggagc ccgccgacgg cgtagtcgat accctcatag aagcctggag ggaggagaaa
     5041 ggcgaggctg agaaacgggc atccgaggac gcgcgcgtca ggcatctcga atccgagctg
     5101 gagcgcctga agcaggacaa cgagcgactc tcgttcgcat cgagggccgc cagagagagg
     5161 gccgccgccc tctcggaaga ggtcgaggtc ctgacaggcg agcgcacgga cacctccgag
     5221 ttggtcagga aggcggagga gagggccgct tcggccctgg atcttcggag ccagctcgac
     5281 gagcgcatcg agaagctcga gaagcggggt gcccagctcg aaaaggcgct ggaaggcgaa
     5341 cgcacggcct actccagggc cgccgagcgc gtcgatgagc tgcacgagga gctcgggcgt
     5401 gttgtcgacg agcgcgacag gataaagaac gccctgcagg acgcccgctt caccgacagg
     5461 ggcttcggag agctcctcgt gcgggccatc aagaacgagg tcgcggccct gcccgcttct
     5521 ctggagagca cggcgcaggc agcacgcctg ctcgagttca tgggcaaggt gctgcaggca
     5581 cacgcggacc tgcggagccc cggggaacca aggaccgtgg aggaagaccc cgacgactcg
     5641 gacccttctc cagccagccg gcctgaagaa accaacgggc cgagaggaag gtccacgttc
     5701 cgcagggcca atggctatgc caggccccgc ccgacgctct ccttccgcgc ccttggcggg
     5761 gccggggagg tcggtggcag cagccacctc ctcgacttcg gcaacaccag gattctggtc
     5821 gacgcaggga tcaagccgga cggacgcggt cccttgaccc ctgacttcgg agggcttgat
     5881 gggatcgatg cagcgatcat aacgcacgcg caccttgacc actgcggggc gctgcccgtg
     5941 cttgtgcgcg acaggcccga catgccgatc tactgtacgc cgccttctgc gaagctcatc
     6001 gtcagcgcgc tcaacgacca cgcggcgatg ggcggcgggc tgcccggcgg ggctcccatc
     6061 cacgaggtcc gcaagcgact cgtccctgta cctttcggca agccgctgga tgtcggggac
     6121 gcgaaggtca cgctcaccga gagcggccac atcctggggg cggcgagcgt actgttcacg
     6181 tcagggccgg ctaccgtctt ccacaccggg gacatctgca tggagaacca cttctcgatc
     6241 ccttcggccc ggcttcccga agtcaaggac atagacctcc tcatcatgga ggcgaccctc
     6301 gccgaccaga aaccacagcc gttctccgag tcgatcagga ctatggtcga ggtcataaac
     6361 gacacgacgc tggagcgggg cgggacggtc ctcatcccga cctacgccct ggggcaggcg
     6421 caggagatca tcctcggcat caagcactac agtaaggagt acggcctgga cagagacgtc
     6481 ttcatctacg tcgacggctc cgtggttacc acatcggaga ggctctacgc cgagcaactc
     6541 gggtacatga agccctatct gcagcacagc gacccgcgtg agatcttctt ctccgagaac
     6601 atcagggccg tcgccaacga cgacagggcg cgcgagcgta tcctctcgaa cccctgcgcc
     6661 atcatcgcct cgccggtaac gatgcagggc ggggcgagcg ctttctaccg caagcgcctg
     6721 gagaagcatt cgaagaacgc cgtgatccta ccctccaacg ccgcctcttc ctacgggacg
     6781 caccgcgccg agggcgagga ggagcagtgg agggtcgagc gggtcagctt tgccgcgcat
     6841 tgcacccagg acgagttgct cggcatcaca gagaaattga gcccgcgtca gatcatcctg
     6901 atccacggct ccaggcgcag gataagcgac ctggccttca gactcgcccc gaaccacaag
     6961 atccacaccc ctgccgtcgg cgagaccgtc cgcaccgtgc tgtagtgtca ggagaactgc
     7021 ctgaggtcat gaggctcctt gacactcgcg gcgagcgccc cgcaaacgcc gccggcatcg
     7081 gggagttctg gtacgagccg gaggtctggg ccctccccct ctccaccgac gcacgtgtgc
     7141 tctacgccgg cctgtgctcc ttcctcgggc accgcgagat caaccgcaag gacctccgca
     7201 acacactcaa aggccacccg gacagtgaga tttccaaggc cctcgaggag ctggtacgtc
     7261 acgatttgct cgtacccgca gacaacggca cctttcgggg ctatgaggtg cgcccggtgg
     7321 gcgatttcag cgtatagttc ctgcatggtt cacgggaccc aggaatctaa tctctcacgg
     7381 tacatgcact tctcacgcga ggagtggagc cggctgcggg ataatacgcc tctcaccctg
     7441 agggaggagg acctcgcctc cctgcgaggt cgcaacgaga aggtctcgct ggaagaagtg
     7501 gttgaggtct acctgcccct ctctcgcctc ttgaacctgc acataaccgc ggctcaggga
     7561 ctctacagcg tgacggactc tttcctcgga aaccccgcgg caaaggtccc gtacgtcctc
     7621 ggcatcgccg gcagcgtcgc cgtcggcaag agcacgacgg cgcgcatcct gcagtccctc
     7681 ctcgccaacc gccctgacca tcctgaggtg gccttgatca ccaccgacgg ctttctctac
     7741 ccgaaccacg tcctcgagga acgcggcttg atgaagagga agggattccc cgagagctac
     7801 gacctcaggc gcctcgtacg ttttgtggcc gacgtcaagg ccggtcaccc cgaggtctcg
     7861 gctccggtct actcgcacct ggcctacgac atactcccag gggagatgca atacgtcaga
     7921 cagcctgacc tgctgatcgt agagggactc aacgtccttc agacggggaa cggagatccc
     7981 aggaatgcgc ctaccgcgtt cgtctccgat ttcttcgact tctcgatata cgttgacgcc
     8041 gaagaatcac acgtcgagcg ctggttttcg gagcgcttcc tgacgctgcg ggacaccgta
     8101 ttcaaggacg agtcctcgta cttccaccgc tacgcccacc tctccacgga ggaagccagg
     8161 cgtgtggcgg ccgggatctg ggccgacatc aacggcgtga acctgcggga gaacatcgtc
     8221 cctaccaggg agcgcgcgca gttgatcctc cagaagggcg aggaccatca ggtcctcggg
     8281 gtgagtcttc gcaaattatg aggcagctat cccggccagc gtaactccgt aggagatctc
     8341 ctcccatcta ggagatcctc tagcgtgtag ggttcgtcgg cccagcgcct gcccgcgccg
     8401 cccgtgaggg aagcaaggtc gatgagccgt ccgagtatcc tgaggcagaa gccgttcaac
     8461 acgtcgtcgg ggtcgtcgac gcgcccgcga gccttcgcga cgaagagccc tctggtcttc
     8521 cacacgccgt cggaatacac ggcgaagacc tggcagggct cgtccccggc ctcctgtgac
     8581 caggcgagca cgcgtccgga gtaacccgac ggctccaggc tgtagcggct caactcctca
     8641 tgcatgaggt tctcgtcggt ctcggccgcc gcgccgggaa ctaccccgaa ggagacgcct
     8701 gcctccgagc ctgctacgac acgcaggtcg agcgcgtcac cggcgtaggt ggtctctatc
     8761 tcgaatccgg cctgccttcc gagtaccgcc gtatatt
//
LOCUS       DATAIR010000212         5600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_105584, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000212 DATAIR010000000
VERSION     DATAIR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105584"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="VHM69_05090"
     CDS             complement(<1..2114)
                     /locus_tag="VHM69_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX2739800.1"
                     /translation="MRVYLIDGYSLLYRAFYALPQSISTSSGLPTNALYGFTSMLLKL
                     LDTEDEVGLGVVWDGGMPKFRLEIFPEYKAQRSAMPEELRSQLDHLDEILDAMNIPAI
                     RAEGFEADDALATMSRQVPEGVELYIVTGDQDAMQLVDGQVKVLRTTRGVSETKTYGR
                     EEVVEEYGVTPEQIPDYKALTGDSSDNIPGVRGIGPKGAANLLEQFGTLEGVYENLEN
                     ISAKGTRKKLEEGRESAFMSLELARMRFDVPVKFDHEALRFEGISPEIRDITRRYEFR
                     TLESRFDTLPVSGGEKLPPRERLEVHVSDEPLDLSFEPGAAAPVGDGRWAVAVSGDEV
                     RLTGGLPDVPIKVHDSKKLGVRGADFDTYLAAYLVKPGLGSYELEALASERGMAEVEV
                     GHEDRAVTEAARRAALVYVLSPRLQAELEEMGLARLYYDVELPLADVLGDIEGIGMPV
                     DASTLEEVGADLEERISSVERHIYEEVGHPFNIGSPKQLGEVLFEEMGIDPIRKTKTG
                     YSTDAKVLQQLAIEHPIADRIIEYRELTKLMGTYIEGLLKLISEDGRIHSTLQQTRAA
                     TGRLSSDSPNLQNIPIRTEMGVRIRDAFTASPGRRLVVADYSQIELRILAHMTGEPAL
                     VESFANGEDIHTRTASEVFDVRMESVTPELRRRAKMVNFGILYGISGFGLATRLGNVH
                     PAEAERYIKRYFERYPKVTDFM"
     gene            complement(2165..2632)
                     /locus_tag="VHM69_05095"
     CDS             complement(2165..2632)
                     /locus_tag="VHM69_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739801.1"
                     /translation="MTLDLRADLYKACQRISPTHSDYATLPIRDGFDWSSSLSGCDFD
                     RLYLIVFRSVRRKSADLQLLREHDDRAYEEALNSGGLLRYFKGSANERGECLSFCLWE
                     SREQARNAAGAASHRSAASLTEQMYESYVLDRYWLKKVVTARGERLDFEPIRI"
     gene            complement(2899..3606)
                     /locus_tag="VHM69_05100"
     CDS             complement(2899..3606)
                     /locus_tag="VHM69_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739802.1"
                     /translation="MLEIENIHSYYGHIEALRGISLTVEKGEVVTLIGSNGAGKTTTL
                     RSIHGILPPREGRIIFDGEEIQGTPAHDMITKGIAQSPEGRKIFHRMTVLENLEMGAY
                     HRNDRDGIREDMDRVFDLFPRLKERMKQESGTMSGGEQQMLAIGRALMSRPKLLLLDE
                     PSMGLAPVLVERIFDIIKDINEQGTTILLVEQNANVALEIATRGYVLETGTIVNAAPA
                     NELREDPKVREAYLGEI"
     gene            complement(3622..4179)
                     /locus_tag="VHM69_05105"
     CDS             complement(3622..4179)
                     /locus_tag="VHM69_05105"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2739803.1"
                     /translation="MGQTFAKIPDDFVGWFEKQPVFFVATSGPGTHINVSPRGLDTFR
                     VLDANRVAWLDLTGSGVETIAHLKAEEAGGRITLMFCAFEGPPRIVRLYGRDRVREPG
                     DYDGLRSRFPDLPGERAVIDVAVDRVSSSCGFGVPLMEFVAQREELVEFARRKGETKL
                     ASYRARKNTESIDGLPGLTTAGETR"
     gene            complement(4181..4975)
                     /locus_tag="VHM69_05110"
     CDS             complement(4181..4975)
                     /locus_tag="VHM69_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739804.1"
                     /translation="MPDDEGHEGAKILVARGISKLFGGLVAVSSVDFDIPERSIVSLI
                     GPNGAGKTTFFNMVSGLYEPSAGTFTFEGSDITKLGAHKRAQLGIGRTFQNIRLFDTM
                     SAQDNVLSGMHTRLKGSILGSIFSTPGHRREERESRKRAGDYLDFVGISGRETFAKNL
                     SYGDQRRLEIARALASEPKLLLLDEPTAGMNPQETARLTDLIRRLREERGISILLIEH
                     EMRVVMSISDRVTVLDHGEKISEGSPQEVREDPRVIEAYLGTARTG"
     gene            complement(4968..>5600)
                     /locus_tag="VHM69_05115"
     CDS             complement(4968..>5600)
                     /locus_tag="VHM69_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2739805.1"
                     /translation="GIPFLPESLDAWKQFGTLDLYPWYYTILVMVLIAIYVNIRLRDS
                     RLGRAWISVREDETAAAAMGINPVTTKLWAYALGAVFGGLAGAFYGSFIASIFPTSFS
                     FNISILVLCMVVLGGMGNIYGVILGAIALQGLNFYLLPKVNEWVHAIGNAIGSPLLSQ
                     ADIPKYNFFLFGIILVLMMLLRPEGIIPNRQRAEELHGEDAGAMQGASRA"
BASE COUNT          997 a         1765 c         1687 g         1151 t
ORIGIN      
        1 tgcatgaaat cggtaacctt cggatagcgc tcgaagtagc gtttgatgta acgctcggcc
       61 tcggccggat gcacgttgcc cagcctcgtg gccagcccga acccggaaat tccgtagagg
      121 atgccgaagt tgaccatctt cgccctccgc ctgagctcag gcgtcacgct ctccatgcgc
      181 acgtcgaaga cctcggaagc ggtacgtgta tgtatgtcct ccccattggc aaaagactcg
      241 accagcgcgg gctctccggt catgtgcgcc aggatccgaa gctctatctg ggaataatcg
      301 gcgaccacga gcctgcggcc aggcgaggcg gtgaaagcgt ccctgatgcg aacgcccatc
      361 tccgtacgga tggggatgtt ctgcaggttg ggcgagtcgc tcgataggcg gccagtggcc
      421 gcccgcgtct gctgcagggt gctatgtatg cgaccgtcct cggagatgag cttcagtagc
      481 ccttctatat aggttcccat gagcttggtc agctcacggt actcgatgat gcggtcggct
      541 atgggatgct cgatggcgag ttgctgcaga accttcgcgt ccgtcgaata gcctgtcttg
      601 gtctttctga tcggatctat gcccatctcc tcgaatagga cctcgccaag ctgtttagga
      661 gatccgatat tgaaagggtg tccgacctct tcgtagatgt ggcgctccac cgaggagata
      721 cgctcctcaa ggtccgcgcc gacttcttcg agggtgctcg catcgaccgg catcccgatc
      781 ccctctatat caccgagcac gtcggcgagc ggcaactcca cgtcgtagta gagcctggcg
      841 agccccatct cctcgagctc cgcttggagc ctaggcgaga gcacgtacac cagcgcagcc
      901 ctcctcgccg cctctgtgac agccctgtcc tcgtgcccga cctcgacttc agccatcccc
      961 ctctccgaag ccagcgcctc gagttcgtag ctcccgagac cgggcttgac gaggtaagca
     1021 gcgaggtacg tgtcgaaatc agccccccgc acccctagct tcttggagtc atgcactttg
     1081 atggggacgt caggcaaacc gccggtgagg cgtacctcgt cgccggaaac ggcgacggcc
     1141 cagcgcccgt ctccaaccgg cgcggccgca cccggctcga acgagaggtc gagcggctcg
     1201 tccgagacgt gtacctccag tcgttccctg ggaggcagtt tctcgccgcc tgagacgggc
     1261 aacgtgtcga agcgggactc tagagtgcgg aactcgtagc gccttgtaat gtcccggatc
     1321 tccggtgaga tgccctcgaa tcggagcgct tcgtggtcga acttcacggg cacgtcgaag
     1381 cgcatcctcg cgagctctag cgacatgaag gcgctctccc tgccctcttc gagcttcttg
     1441 cgagtgccct tcgcggagat attctccagg ttctcgtaga cgccttccaa cgtgccgaac
     1501 tgctcgagga ggttcgccgc gcccttcggg ccgatgccgc gcacgccggg gatgttgtcc
     1561 gaagaatctc ccgtgagggc tttatagtcg gggatctgct ccggcgtaac accgtactcc
     1621 tcgacgacct cctcgcgccc gtaagtcttc gtctcggaga cgcccctggt cgtgcgaagt
     1681 accttgacct gtccatccac gagttgcatc gcgtcctggt cgccggtaac tatataaagc
     1741 tcaacgcctt cgggcacctg tctcgacatc gtcgccaggg catcgtcggc ctcgaagccc
     1801 tcggccctga tggcaggaat gttcatggcg tccaggatct catcgaggtg gtcgagctgc
     1861 gacctgagct cctcgggcat agccgagcgc tgcgctttgt actcggggaa gatctcgagc
     1921 ctgaacttcg gcatcccccc gtcccagacg acaccgagac cgacctcgtc ctcggtgtcg
     1981 agcagcttca gcaacatgct cgtgaagccg tagagggcgt tcgtcggcag tccgctggag
     2041 gtgcttatgc tctgcggcag cgcgtagaac gccctgtaca ggagtgagta gccgtcgatc
     2101 agataaactc gcactgccga aattataccg taggggcggt ccgattcccg ccccgatcgg
     2161 gaccttagat ccgaatcggc tcgaagtcga gcctctctcc tcgtgcggtg acgaccttct
     2221 tcaaccagta acgatcgagc acgtaggatt cgtacatctg ctcggtaagg ctagcagcgg
     2281 accggtgcga ggcggctccg gcagcattcc ttgcctgttc gcggctctcc cacaggcaga
     2341 aggagaggca ttctccacgc tcgttcgcgc tccctttgaa gtagcgcagc aaaccgcctg
     2401 agttcagagc ctcctcgtac gcgcggtcgt cgtgctcgcg caggagttgg agatccgcac
     2461 tcttccgcct gacggagcgg aagacgatca ggtaaaggcg gtcgaagtcg cagcctgaga
     2521 gggaggaaga ccagtcaaag ccgtcccgga tgggtaacgt cgcgtagtct gaatgcgtgg
     2581 gggagattcg ctggcaagcc ttgtaaaggt ccgcccggag atcgagggtc ataagttacc
     2641 tttctggatt cttctctaaa agtcacctcc tctagttgtc tgcagacaac ggaaccggcc
     2701 cttcttgccg gtcacggtcc cacgtacgtc gccaggctgc ctgcgggacg tgtgcccgaa
     2761 cccgtagccg ctccaggcga gctgcttgag ccggtacgaa ggcgcgtaga acgaatgccc
     2821 catatccaag cgctcgcgtg cgttaatgcc cgacatggcc acagtgtaca ttagttcact
     2881 gatactatga agaaatccct aaatctcgcc caggtatgct tcgcggacct tcgggtcttc
     2941 gcgcagctcg tttgcggggg ctgcgttgac gatggtgcct gtttcgagga cgtagccgcg
     3001 ggtggcgatc tccagggcga cgttggcgtt ctgttcgacg agcaggatcg tggtgccctg
     3061 ttcattgatg tccttgatga tgtcgaagat acgctcgacc aggacgggag ccaggcccat
     3121 cgagggttcg tcgagcagca gcagtttcgg gcggctcatc agggcgcggc cgatggcgag
     3181 catctgctgc tcgccgccgc tcatcgtgcc cgactcctgc ttcatgcgct ccttcaggcg
     3241 cgggaacagg tcgaagacgc ggtccatatc ctcgcggata ccgtcgcggt cattgcgatg
     3301 gtatgccccc atctccaggt tttcgaggac cgtcatgcgg tggaagatct tacgtccctc
     3361 ggggctctgg gctatgccct tcgtgatcat gtcgtgggcg ggggtgccct gtatctcctc
     3421 gccgtcgaag atgatcctgc cctcgcgcgg gggcagtatg ccgtggatgg agcggagggt
     3481 cgtcgttttc cctgccccgt tagagcctat gagcgtgacc acctctccct tctcgaccgt
     3541 gagtgagatc ccccgcaggg cctcgatatg accgtagtag ctgtggatgt tttcgatctc
     3601 gagcaggggc atagcttctt cctacctcgt ctccccggcg gtcgtcagtc caggcaggcc
     3661 gtcgatgctc tcggtattct tgcgcgccct gtaggacgcc agcttcgtct cccctttgcg
     3721 cctggcgaac tcgacgagct cctcgcgctg tgcgacgaac tccatgagcg gcacgccgaa
     3781 cccgcatgaa ctcgataccc ggtccaccgc tacgtctatg acggcccgct ctccagggag
     3841 atcagggaac ctggagcgta gcccgtcgta gtcgccgggc tcgcggaccc tgtcccgccc
     3901 gtagaggcgg actatcctcg ggggaccttc aaaggcgcag aacatgagcg tgatgcgccc
     3961 tcccgcttct tccgccttga ggtgcgcgat cgtctctacg ccgcttcccg tcagatccag
     4021 ccatgcgacg cgattcgcgt ccagaacccg gaaggtgtcg agtccgcgcg gcgagacgtt
     4081 gatgtgcgtc ccgggacctg aggtggcgac gaagaacacc ggctgctttt cgaaccagcc
     4141 gacgaaatcg tccgggatct ttgcgaacgt ctgacccatc ctaacctgtc ctggccgtgc
     4201 cgagatacgc ttcgatgacc cgagggtcct cgcgcacctc ctggggggag ccttcggaga
     4261 tcttctcccc gtggtccagg accgtcaccc tgtctgagat actcatgacc acgcgcatct
     4321 catgctcgat gagcagaatg gagatcccac gttcttctct gaggcgccgg atcagatccg
     4381 tcagcctggc cgtctcctgc gggttcatgc cggccgtcgg ctcgtcgagg agaagcagct
     4441 tgggctcgga agcgagcgcc cgcgctatct cgaggcgccg ctggtcgccg taggagaggt
     4501 tcttcgcgaa cgtctctcgc ccgctgatac cgacgaagtc gaggtaatct ccggccctct
     4561 ttctggattc cctctcctct cgacgatgcc ctggcgtact gaagatcgaa cccaatatgg
     4621 agcctttgag gcgggtgtgc atccccgaga gcacgttgtc ctgggcgctc atcgtgtcga
     4681 agagcctgat gttctggaac gtgcgtccga tgcccagctg cgccctcttg tgggcgccga
     4741 gcttggtgat gtcgctgccc tcgaaagtga aggtgcccgc gctgggctcg tagagtccgc
     4801 tgaccatgtt gaagaacgtc gtcttgcccg cgccgttggg cccgatcagg ctgacgatag
     4861 agcgttcggg gatatcgaag tcgacggagc tgacggccac gagcccgccg aagagcttgc
     4921 ttataccccg tgctacgagg atcttcgcgc cttcgtgtcc ttcatcgtca ggcacgggac
     4981 gctccttgca tcgcgccggc atcttcaccg tgcaactcct ccgcgcgctg gcggttggga
     5041 atgatgcctt ccggtctcaa gagcatcatc aggaccagga ttatgccgaa caggaagaag
     5101 ttgtacttcg ggatatccgc ctggctgaga agaggactcc cgatggcgtt gccgatggcg
     5161 tggacccact cgttgacctt cggcagcaga tagaagttca gaccctgcaa ggctatggct
     5221 cccaggatga caccgtatat gttgcccatg ccaccgagca cgaccatgca aagcaccagg
     5281 atcgagatgt tgaaactgaa gctcgtcggg aagatgctcg cgataaacga gccgtagaaa
     5341 gccccggcca gtcctccgaa gaccgcaccg agggcgtaag cccacagctt cgtcgtcacc
     5401 gggtttatgc ccatcgccgc ggcggcggtt tcgtcctcgc gcaccgagat ccaggcccga
     5461 cctagccgtg agtccctcag acggatgttc acgtagatgg cgatcaggac catcaccagt
     5521 atcgtgtagt accaggggta aaggtccagg gtcccgaact gcttccaggc gtccagagac
     5581 tcggggagga atggaatgcc
//
LOCUS       DATAIR010000213         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_428520, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000213 DATAIR010000000
VERSION     DATAIR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428520"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..329)
                     /locus_tag="VHM69_05120"
     CDS             complement(126..329)
                     /locus_tag="VHM69_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2739806.1"
                     /translation="MKAVFAVTDACAGCGACIKTCPEKAIHPAPRGHDVPLVVIEDRC
                     SGCAECAEVCPVAACVEVLLETE"
     gene            complement(326..2011)
                     /gene="cobJ"
                     /locus_tag="VHM69_05125"
     CDS             complement(326..2011)
                     /gene="cobJ"
                     /locus_tag="VHM69_05125"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX2739807.1"
                     /translation="MIGLIAATANGRRNAEHLADVWPEARYYEGRAKEALQKAWNECD
                     GIVLFLATGAAVRLVAPLLESKHQDPGVVTVDDAARFAVALCGGHDGGANALAARVEE
                     ALGATPVITTASDSIGVPALDSLGRNLGLRLEEGSDLAPVGAALVSGEKVHLVSDRRW
                     PLGPLPENVARSDVPEVPLIFVSDRFVETPHPAVVYRPPSLVAGIGCSRDTGADEILD
                     LLALSLEEARLSEKSVAALATIDVKSEEAGLLEVARSLGVPVHFHPAEVLSAVEAPNP
                     SEVVRDAVGTPSVAEAAVISCGAELIVEKRKSANVTVAMGRLPVRGRLALVSLGPGDD
                     ALIPPLARDALVSSELVVGLDQYVDRVRHLLRQGTRVLTPPLGSELERAELALAEARS
                     GGSAALVSSGDVGVYAMASPVLELAGEEVDVVVVPGVTAAQAAASLLGSPLGHDHCSI
                     SLSDLLTPWEVIQDRVMAAAEGDFVISLYNPRSKGRDWQLGKVREVLLRHRSPDTPVG
                     IVKDAYRPAQEVTLTDLASLRQEDVDMLTIIVVGSSQTRVVAGRMVTPRGYMA"
     gene            complement(2008..3204)
                     /gene="cbiE"
                     /locus_tag="VHM69_05130"
     CDS             complement(2008..3204)
                     /gene="cbiE"
                     /locus_tag="VHM69_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX2739808.1"
                     /translation="MSRITVVGLDGRPPDEEIEDLLRDAALVAGGERHLKMLGVKRGV
                     VLKGDLSEALARIEGTEGPVVVLASGDPGFFGIVRLLGGRFGRENLRVVPGLSSVALA
                     FARVGLSWDDAVTVSAHGRDPRRGVNVCRAHPKVAVLTSPDFGPAELAQTLDGLGRTF
                     VVAEKLGWPDERVFQGDATTVAGMQWEDPNVVLILERGQNSGSKGWISSGVRSPERWG
                     LSEDQFEHRSGMITKSATRAFVLSQLGPGRGDLIWDVGSGSGSVAIECARLGAATIAI
                     ERDPESCARILRNTERHGVYVQLIEDEAPGALRDLPEPDSVFVGGTGGDFEEIVKLAA
                     VRARRAVVLALVGLERVVPAAHILEDCGFEVETTFLQTSRMKEVGSLHRLAAESNVLI
                     VSGSRP"
     gene            complement(3201..4367)
                     /locus_tag="VHM69_05135"
     CDS             complement(3201..4367)
                     /locus_tag="VHM69_05135"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX2739809.1"
                     /translation="MPPRKLREPSMPPSMEKVKGDKLRTGWTTGTCAAAAAKAAACAL
                     VSSEPQVRVDVKLPKKGAERRVEFEVERCELGDSWAEAVVVKDAGDDPDVTDGAHLTA
                     RVSWREESGLALERGEGVGVVTKPGLGLPVGGPAINAVPKRMISYSVEEVLDPGERGV
                     RVVISVPGGEKMAEKTTNARLGIVGGISILGTTGIVRPFSTAAWAASVVQAVNVMGAQ
                     GHKTFVLSTGGLTEKAAMRLLPDLEEVNFVEVGDFTGQAMKRAVENNLERCFFVGMVG
                     KISKLAAGVMMTHWTRSKVDNDLLAEITTKAGGSPDLIGEVKNANTARHAYELWRSAK
                     LNGAADLLCRQAAENLDEYAEGKLEVHVILVDFDTLDPAGASPGALELTTWSPS"
     gene            complement(4354..5133)
                     /gene="cobM"
                     /locus_tag="VHM69_05140"
     CDS             complement(4354..5133)
                     /gene="cobM"
                     /locus_tag="VHM69_05140"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX2739810.1"
                     /translation="MWFIGAGPGSPDLMTVRGAKLIGEADVVIWARSLVHEEILEYAR
                     PDAQIVESTTIPLEGVRGLYEWAVDEDLKVARVHSGDPSLYGAVMEQIELCDEIGLEW
                     EIVPGVSSLGAAAAAIGRELTVPEVSQSVILTRRASRTPMPEGEDIRGFAQHGTTMAI
                     FLSAAKPRALQEELLEGGYAPETPCAVVYRASWPDEEVIECRLEDLAHRIREAGFTRQ
                     ALILVGPGLGAGGTRSHLYSPEFSHMFRRAKPAEKGADAAT"
BASE COUNT          913 a         1852 c         1616 g          915 t
ORIGIN      
        1 ttcaagatcc gccgaagcgt gaatgattcg ttccgccacg gcgcgggaga gcggcccgag
       61 atgcgagaga tccaccatcc cgcgcaggat gcggtagctc tcggtctcta ttggatggac
      121 gcgcttcatt cggtctccag caatacctcg acgcaggcgg cgaccgggca gacctcggcg
      181 cactcggcgc atcccgagca acggtcttct atgacgacga ggggtacgtc gtggcccctg
      241 ggagccgggt ggatcgcctt ctcaggacag gtctttatgc aggcgccaca accagcgcag
      301 gcgtctgtca cagcgaatac cgccttcacg ccatgtaccc tctcggagta accatgcgac
      361 cagccacgac ccttgtctga gagcttccga cgacgatgat cgtcagcatg tccacatctt
      421 cctggcgcag agaggcgagg tccgtgaggg ttacctcctg cgcagggcgg taggcgtcct
      481 tcacaatgcc gaccggcgta tcaggcgaac ggtgtctgag caggacctcc ctgaccttcc
      541 cgagttgcca gtcgcggccc ttcgagcgcg ggttgtagag tgagatcacg aagtcccctt
      601 cggccgccgc catgacccgg tcctggatca cctcccacgg cgtgagcagg tctgagaggc
      661 taatggaaca atggtcgtgc ccgagcggcg agcccagcaa cgaggcagcc gcctgcgccg
      721 cggtcacgcc aggaacgacg accacatcca cttcctcgcc agcgagctcg agaacagggg
      781 aagccatcgc atacacccct acgtccccgc tcgacacgag cgcggcgctg ccaccggagc
      841 gagcctcggc gagcgccagt tccgcccgct ccaactcgct gcccagcggc ggcgtaagta
      901 cccgcgtacc ctgccgcagc agatgcctca cacggtccac atactggtcc agcccgacga
      961 ctagctctga agagacgagg gcatcgcggg caagcggcgg gatgagggcg tcatcccccg
     1021 gtcccaggct aaccagtgcg agccgcccgc gcacaggcaa ccgtcccata gcaaccgtaa
     1081 cgttcgccga tttgcgcttc tcgacgatga gctcggcgcc gcaggagatc accgccgcct
     1141 ccgccacgct cggtgtcccg accgcatccc ttaccacctc cgacggattt ggcgcctcca
     1201 ccgcagagag cacctcggca gggtggaagt ggaccggcac gccaaggctc ctggcgactt
     1261 cgagcaaccc tgcttcttcg ctcttcacgt cgatggtagc gagggctgcg acgctcttct
     1321 cggacaacct ggcctcctcc agagacagag cgagcagatc gagtatctcg tcagcgcccg
     1381 tgtccctgct gcaaccgata ccggcgacga gcgagggtgg acggtagacg actgccggat
     1441 gcggagtctc tacgaaccgg tcggagacga agatgagtgg cacctctggc acgtccgaac
     1501 gcgcgacgtt ctccggtagc ggcccgagcg gccaacgcct gtcggagacg aggtggacct
     1561 tctcgccgga gacgagtgca gccccgaccg gcgcgaggtc cgagccttct tccaggcgga
     1621 gaccaagatt ccttcccaga gagtcgaggg ctggaacgcc tatcgagtcg cttgcggtgg
     1681 tgatcaccgg cgtcgcgccc agtgcctcct cgacccgtgc ggcgagagcg ttcgcgccgc
     1741 cgtcgtgtcc tccacagagg gcgacggcga atctcgcggc gtcgtccacg gttaccacgc
     1801 cgggatcctg atgcttgctc tccagtagag gagcgacgag acggacggct gccccggtag
     1861 cgaggaagag cacgatcccg tcgcactcgt tccatgcctt ctgaagcgct tctttagccc
     1921 tgccttcgta gtagcgcgct tcgggccaga catcggcgag gtgctcagcg ttgcggcggc
     1981 cgttcgcggt ggctgctatg agcccgatca cggtcggctc cccgagacga tgaggacgtt
     2041 gctctcggcg gcaaggcggt gcaaactacc gacttccttc atcctggacg tctgcaggaa
     2101 ggtcgtctcg acctcgaagc cgcagtcttc gagaatgtgc gcggccggga cgaccctttc
     2161 cagccccacg agagccagca cgaccgcccg gcgtgccctg acggctgcta gcttgacgat
     2221 ctcctcgaaa tcgccgcccg tcccgccgac gaagacggag tctggctcgg gcaggtctcg
     2281 cagagccccg ggggcctcgt cctcgatgag ttgcacgtag acgccgtgcc gttcggtgtt
     2341 gcggaggatt cgggcgcaac tctcggggtc gcgttcgatg gcgatagtgg cggcgcccag
     2401 acgggcacac tcgatggcaa ctgagccgct gccagaaccg acgtcccaga tcaggtctcc
     2461 tctaccgggc ccgagttgcg atagaacgaa agcgcgggtc gcggacttgg tgatcatgcc
     2521 cgacctgtgc tcgaactggt cttcggatag cccccaccgc tctggactcc ggacgcccga
     2581 tgagatccaa cctttggacc cagagttctg ccctcgttcc aggatcagta ccacgttcgg
     2641 gtcttcccac tgcatgcccg cgaccgttgt ggcgtcgcct tgaaacaccc gctcatcagg
     2701 ccaaccaagc ttctcggcga cgacgaaggt gcggccgaga ccgtcgagcg tctgcgcgag
     2761 ctcggccggt ccgaagtcag gggatgtgag gacggctacc ttcggatgcg cgcggcagac
     2821 gttgacgccg cggcgaggat cgcgcccgtg ggcgctgacc gtgaccgcat cgtcccacga
     2881 gagaccgaca cgggcgaaag caagcgccac cgagctgagg ccaggcacga cccgcaggtt
     2941 ctccctcccg aaccgcccgc cgagcagacg cacgatgccg aagaagccgg ggtcccccga
     3001 cgcgagcacg accacaggtc cctcagtacc ttcgatccgc gcgagcgcct ccgagaggtc
     3061 tcccttcaaa actacgccgc gcttcacacc gagcatcttc aggtgccgct cgccgccggc
     3121 aacgagcgcc gcgtcccgca gcaggtcttc gatctcctcg tcaggcggtc ttccatcgag
     3181 accaacgacg gttatgcggc tcacgaggga ctccaggtcg tcagttcaag ggcaccgggg
     3241 ctcgctccag cggggtcgag ggtgtcgaag tctacgagga tcacatgaac ctctagttta
     3301 ccttcagcgt actcatcgag gttctctgcg gcctgcctac aaagaaggtc cgcggcccca
     3361 ttcagcttcg ccgaccgcca gagctcgtag gcgtggcggg cggtattggc gttcttgacc
     3421 tccccgatga gatcggggga gccaccggct ttcgtcgtga tctccgccag cagatcgttg
     3481 tctactttcg agcgggtcca gtgggtcatc atgacgccgg cggcgagctt ggagatctta
     3541 cctaccatcc cgacgaagaa gcaacgctcc aggttgtttt ccacggccct cttcattgcc
     3601 tgtcccgtaa agtcccctac ctctacgaag ttcacctctt cgaggtcggg caacaggcgc
     3661 atcgccgcct tctctgtgag accgccggtc gagaggacga aggtcttgtg cccctgcgcg
     3721 cccatgacgt ttacggcctg cacgacgctt gcggcccacg ccgccgttga gaatgggcga
     3781 actatacctg tggtgccaag gatggatatg ccgccgacga tgccgaggcg tgcgttggtc
     3841 gtcttctcgg ccatcttttc gccgccgggg acgcttatga cgacgcggac gccgcgctcg
     3901 ccggggtcca ggacttcttc cacggagtag gaaatcatac gcttcggcac cgcgtttatg
     3961 gccggtcccc cgacgggaag accgagcccc ggcttcgtga cgacgccgac gccctcgccc
     4021 cgctccagag ccagacccga ctcttcccgc cacgagacgc gggccgtgag gtgcgcgccg
     4081 tcggtcacgt cggggtcgtc gcccgcgtcc ttgacgacga cggcctcggc ccaggaatct
     4141 cccagctcgc accgctccac ctcgaactcg acgcgccgct ccgcgccctt tttgggcaac
     4201 tttacgtcga cgcggacctg cggctcgctg ctcacgagcg cgcaggcggc ggccttggcg
     4261 gcagcggcag cgcaggtacc tgtggtccag ccggtcctga gcttatcgcc cttgaccttc
     4321 tccatgctcg ggggcatgga cggctcgcgg agcttacgtg gcggcatctg cccccttctc
     4381 cgcaggcttc gcccggcgga acatgtgcga gaactcgggg ctgtagagat gtgagcgtgt
     4441 cccaccagcc ccgagcccgg gtccgaccag gatgagcgcc tgcctggtga agccggcctc
     4501 cctgatcctg tgggcgaggt cttcgagacg gcactcgatg acttcctcgt caggccagct
     4561 cgcccggtag acgacggcac acggagtctc cggtgcataa ccgccttcca ggagttcttc
     4621 ctgcagcgcg cgcggcttcg cggcagagag aaagatcgcc atggtcgtcc cgtgctgcgc
     4681 gaaacccctt atgtcctcgc cttcgggcat cggggttcgg ctcgcgcggc gcgtaaggat
     4741 caccgactgg gatacttcgg ggacggtcag ctcgcgccct atggctgcgg ccgcagcgcc
     4801 tagcgacgac acaccgggaa ctatctccca ctccagccct atctcatcgc agagttcgat
     4861 ctgctccatc acagccccgt acaggctggg gtcgcccgag tgcaccctgg cgaccttcag
     4921 gtcctcgtcc acggcccatt catagagccc gcgcacgcct tcgagcggta tggtcgtcga
     4981 ttcgacgatc tgcgcatcag gccgggcgta ttccagaatt tcttcgtgga cgaggctcct
     5041 ggcccagatc accacgtcag cctcgccgat gagcttcgcc cccctgaccg tcatcaggtc
     5101 cggtgacccg ggtccagccc cgataaacca cactttaccc aaactccgag ctcctctcgg
     5161 taaagatcac agtagataga taggttccct cccgcccgac catctcccgc gcagcaacga
     5221 cctcttcacc ctcgatgccg agccgggccc cgtacacacc gccctccaac cgtcctccct
     5281 cctccgcaac cccgag
//
LOCUS       DATAIR010000214         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_13557, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000214 DATAIR010000000
VERSION     DATAIR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13557"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VHM69_05145"
     CDS             <1..571
                     /locus_tag="VHM69_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2739811.1"
                     /translation="LAPFTYLAAKALTFIFLPWAGYPLNVASGSLFGLFWGYVLTATG
                     DTLGGCILFGLSRWKGRPVVARIVGESRMARVDGVLDRGLGGWQELLFFRVVVPIPYN
                     FVSLAAGLAPSLRLRQYVLVTFLTTIPKVFTVGIGAGLVTGEWMEVAVAGLLAVIAVA
                     ALLARRDIRKALKRALWWRWEERRLKGRS"
     gene            complement(594..1649)
                     /locus_tag="VHM69_05150"
     CDS             complement(594..1649)
                     /locus_tag="VHM69_05150"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX2739812.1"
                     /translation="MIEIGVIGGDGIGPEVVHAGMKVLEAAASDDPSLELGFTEFPWG
                     CEYYLEHGRMMPEDGLESLAACDEIYLGAVGWPTVPDHVSLWGLLLPIRRTFDQYVNL
                     RPARLLPGVRSPLADPRDLDITVVRENTEGEYSDSGGRVYKDTPYEIAVQESIFTRRG
                     VERIIRYAFEEAKGRRGLLVGATKSNGISITMPFFDEIFNEITADYPDVEASLMHADA
                     LAARLVLAPQSFDVVVGSNLFGDLLSEITAAIAGAIGIAPSANLNPEGTYPSLFEPIH
                     GSAPDIAGEGIANPAGAIWAGAMMLDHAGYPDAGARVLTALEETLASGTKTRDLGGTA
                     STDEMADAVTGRLLNAV"
     gene            2002..2337
                     /locus_tag="VHM69_05155"
     CDS             2002..2337
                     /locus_tag="VHM69_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739813.1"
                     /translation="MDERDVKRARNASKDLDRLTRESYKTAVDRAFDAQKSGMRLSRR
                     FFENWVETLEDQAELNRRTLGDLQELVREQREVFRALSRESVDAYDGFLDSLDAYEQE
                     VSKLEGRED"
     gene            2427..>3451
                     /locus_tag="VHM69_05160"
     CDS             2427..>3451
                     /locus_tag="VHM69_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739814.1"
                     /translation="MREEELQKSLSGRPGTRSFDGVNPRAADVLRRNGLKSWTKPAPS
                     LYPHQWSWDSAFIALGLAHVDNRRATDELETLFRGQWSTGKVPHIVFNPEAPPKSYFP
                     DAERWNSSDLSKDAPTGPRTSGLCQPPVHAIAVRRIWQTARGKDEERVARARSFLGGN
                     YPRLFSWHRYLATARDPEGSGLVTIYHPWESGTDNSPRWDATLDRISVGDVPAYTRYD
                     LQHVSDPSHRPTDLEYDRFLWLLELLKRARYDEADIYERHPFLIKDVLFSAILVAANE
                     ALLEISEVVDAPREDRALIEGWIERGRAGLEDQWDPDLGLCLDRDVLAGESVQVRTIA
                     GFAPLIAG"
BASE COUNT          602 a         1059 c         1130 g          660 t
ORIGIN      
        1 cctggctccg ttcacgtacc tcgcggcgaa ggcgctcacg ttcatcttcc tgccgtgggc
       61 tggctacccg ctcaacgttg cctcgggctc tctcttcggg ctcttctggg ggtacgtact
      121 cacggcaacg ggggataccc tggggggctg cattctgttt ggattgtcga ggtggaaagg
      181 ccggcccgtc gtggcgcgga tcgtgggtga gagccgcatg gcgcgcgtgg acggggtact
      241 cgaccgcggc ctcggcggct ggcaggagtt gctcttcttc agggtagtcg tgcccattcc
      301 gtacaatttt gtcagcctcg ccgccggtct ggctcccagt ctgcgcttac ggcagtacgt
      361 actcgttacc ttcctgacga cgatcccgaa agtcttcacg gtggggatcg gggccggcct
      421 cgtcaccggt gagtggatgg aggtggcagt cgccggcttg ctagctgtga tcgccgtcgc
      481 ggcgctgctc gcgcggcgcg acatcaggaa ggctctgaag cgcgccctgt ggtggcgctg
      541 ggaagagagg cggctcaagg gacgttcata gagcgtcctg gtgttgctat cgttcacacc
      601 gcgttcaaga gtcttcctgt aacggcgtcg gccatctcgt ccgtactcgc cgtgccgccg
      661 aggtcgcggg tcttcgtacc tgatgcgagg gtctcttcga gggccgtgag gacccgcgcg
      721 ccggcgtccg ggtatcctgc gtggtcgagc atcatggcgc cggcccagat cgcgcccgcg
      781 gggttcgcga taccctcgcc ggcgatgtcg ggggccgagc cgtggatggg ctcgaacagg
      841 gaaggataag ttccttcagg gttcaggttg gctgaaggcg cgatcccgat cgcccctgcg
      901 atggcggccg ttatctcgga gagcaggtct ccgaagaggt tggagccgac gacgacgtcg
      961 aacgattgtg gagcaaggac cagcctggca gccagggcgt cggcgtgcat cagtgaggcc
     1021 tccacgtcgg gatagtcggc ggtaatctcg ttgaagatct catcgaagaa aggcatcgtt
     1081 atgctgattc cgttcgactt ggtagccccg acgagcagac ctcgtctacc cttcgcctcc
     1141 tcgaaggcgt aacgtattat cctctccacg ccgcggcggg tgaagatgct ctcctgtacg
     1201 gctatctcgt acggcgtatc cttgtacaca cgcccgcccg agtccgagta ctctccctcg
     1261 gtattctccc ttaccacggt gatgtcgagg tctctcgggt cggcaagggg gctcctcacg
     1321 cccggcaaca ggcgtgccgg acgcaggttg acgtactggt cgaaggtgcg tcttataggg
     1381 agcagaagac cccagaggga gacgtgatcc gggaccgtcg gccagccgac ggcgccgagg
     1441 tagatctcat cgcatgctgc cagggattcg agtccgtctt caggcatcat gcgcccgtgt
     1501 tccaggtagt attcgcagcc ccacgggaac tccgtaaatc ccagttcgag cgagggatcg
     1561 tcacttgcgg ccgcctcgag aaccttcatt ccggcgtgca cgacctcggg gccgatgccg
     1621 tcgccgccga tgactccgat ctcgatcact gcctcgctcc accggaaaag ttcatgggat
     1681 gcctcctctt tgctcgccca tcttccgacc catgctagca accaacaacg tccccgttca
     1741 acggtggccc gtctgccggg cagccccagc ggctgcactt cggcacaaga agatcacata
     1801 agtacggcgt gtttcaaagg cgcgtggcgt cgaaatcgtt gcccacagcg gcgagatcta
     1861 accgctcttc actgcgcccg ctctacttgc agggccatca gcagaatgaa gggaaaatgt
     1921 aatgtcggcg aggaatgtta agggttttgg tcgtaccggg catgagcggt tatcataagg
     1981 gtttggataa ggaggcaggt tttggacgag agggacgtca agcgggccag gaatgcctcg
     2041 aaggacctgg ataggctcac acgcgagtcc tacaagacgg cggttgacag ggcattcgac
     2101 gcccagaaga gtggcatgag gctctcccgg agattcttcg agaactgggt cgaaactctg
     2161 gaggaccagg ccgagttgaa ccgccgtacg cttggtgatc tgcaggagct ggtcagggaa
     2221 cagcgcgagg tcttcagggc gctgtcccgg gagtcggtcg acgcttacga cggcttcctg
     2281 gactccctgg acgcctatga gcaagaggtc tccaagctag aggggcgcga ggattgagcg
     2341 cgatatcttc cggcggggct tgaaggcagg ccggtcgttg tatacattgt ccgggtcaag
     2401 ctaggcgacg ggagtagcac cggttcatgc gagaggaaga gcttcaaaaa agcctttccg
     2461 gacggccagg gacccgcagc ttcgacggcg taaacccgcg cgcggcggac gtcttgcgcc
     2521 gcaacggctt gaagtcgtgg acgaagcccg ctccttctct gtatccgcac cagtggagct
     2581 gggattcggc gtttatcgcg cttggtctgg cgcacgtgga caaccgccgg gcgacggacg
     2641 agctggagac cctgttcaga gggcagtggt caacgggcaa ggtcccgcac atagtcttca
     2701 accctgaggc accgcccaag agctactttc ctgacgcgga gcgttggaac tcctccgatc
     2761 tctcgaagga cgcgcccacg ggcccgcgca cgagtggtct ttgccagccg cccgtacacg
     2821 ccatagccgt ccggcgcatc tggcagaccg ccaggggcaa ggatgaggaa cgcgtcgccc
     2881 gcgcccgctc cttcctcggc ggcaactacc cgcggctttt ctcctggcac cgctaccttg
     2941 cgaccgcgcg ggaccccgag ggatccgggc tggtgaccat ctaccatccg tgggagagcg
     3001 gtacggacaa ctccccgcgc tgggacgcga ccctcgacag gatctccgtg ggggacgtgc
     3061 cggcgtatac ccgctacgac ttgcagcacg tctcggatcc ctcacaccgc ccgaccgacc
     3121 tcgagtacga ccgctttctg tggctgctcg agttgctcaa gcgggcccga tacgacgagg
     3181 ccgatatcta cgagaggcac cccttcctca tcaaagacgt gcttttcagt gcgatcctgg
     3241 tcgcggccaa cgaggcgctg ctcgagatct ccgaggtcgt cgacgcgccg cgcgaggaca
     3301 gggcgctcat agagggttgg atcgaacgcg gacgcgcggg cctggaggat cagtgggacc
     3361 ctgacctcgg gctctgcctg gacagggacg ttctcgctgg cgagtctgtg caggtgagaa
     3421 ccatagccgg cttcgcgccc ctgatcgccg g
//
LOCUS       DATAIR010000215         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_190492, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000215 DATAIR010000000
VERSION     DATAIR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190492"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VHM69_05165"
     CDS             <1..692
                     /locus_tag="VHM69_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2739815.1"
                     /translation="YTSPTAEFWFGTDEFGRDMFARLWEGTRVSLYIAFLAAFLDLAV
                     GVIYGAVSGLLGGRVDSVAQRIIEILVGIPSLVVAILAMVVFDPGILTLSIAIGLTGW
                     VTMARIVRGRVMQLKEQEFTLASRSLGAGKRRLLLRHLIPNSLGPIIITVMFTVPSAV
                     FYEAVLSFIGLGIQVPNASLGALIDSGAGEMRFHPYLLLFPAAVFSLLMLCFNVFGDG
                     LRDALDPRMRR"
     gene            694..1722
                     /locus_tag="VHM69_05170"
     CDS             694..1722
                     /locus_tag="VHM69_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2739816.1"
                     /translation="MATQGESILEVNDLRISFSTYAGEVQAVRGASFELRHGETLAIV
                     GESGSGKSVAARSIMRLNPEANTIVRGGEILFEGEDLLKLSEKQMQEIRGPKIAMVFQ
                     DPMTSLDPTMRIGRQISESLKKHLGLSGRAAAERSVELLKLVGIPNPEHRLKQYPHQF
                     SGGMRQRVVIAIALSCDPQILIADEPTTALDVTIQAQILELLRELQDRLGMSIILITH
                     DLGVVASTAHRIGVMYGGMIVETGTVRDIFYNPQMPYTWGLLASIPLPTADRSLELIP
                     IPGSPPSALHPPKGCPFTARCPYAMRICVDEMPEYTTFSDEHRAACWLHHQMAPNVEP
                     PARVRGTR"
     gene            1719..>2404
                     /locus_tag="VHM69_05175"
     CDS             1719..>2404
                     /locus_tag="VHM69_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2739817.1"
                     /translation="MTEQNGSGGENDRPLLEIKDLRKYFKVGREDLKAVDGISLDIKK
                     GETFGLVGESGCGKSTAGRVLVRLYDPSSGEVLYGGKNIHEATGDEGRVLNRKMQMIF
                     QDPQASLNPRMIVGDIIAEGIDIHGLVASRKERTERVQELLDTVGLSKEHASRYPHEF
                     SGGQRQRIGVARALAVDPDFIVADEPVSALDVSIQAQVINLMKRLQRERDLTYLFISH
                     DLSVVKYISDR"
BASE COUNT          462 a          762 c          760 g          420 t
ORIGIN      
        1 cctacacctc gcccacggca gagttctggt tcggcacaga cgagtttggc cgagacatgt
       61 tcgcccgtct ttgggaaggg accagagtct cgctctacat agcgttcctc gcggcctttc
      121 tagacctggc cgtgggcgtt atctacggcg cggtctccgg tttgctcggt ggcagggtgg
      181 acagcgttgc gcagcgcata atcgagatac tcgtcggcat cccaagcctc gtggtggcca
      241 tcctggcaat ggtggtcttc gatcccggga tcctcacgct gagcatagcc atcgggctga
      301 ccggctgggt gacgatggcc aggatcgtaa ggggccgggt catgcagctc aaggagcagg
      361 agttcaccct cgcttcgcgt tcgctcgggg caggcaagcg caggctcctc ctcaggcacc
      421 tgatccccaa ctcgctgggg ccgatcataa tcacggtcat gttcacggtg ccgagcgccg
      481 tcttctacga ggcggtattg agctttatcg ggctcggaat ccaggtgccc aacgcgtcgc
      541 tcggggccct gatcgactcc ggcgccggcg agatgcgctt ccacccatac ctcctgctct
      601 tcccggccgc cgtcttcagc ctgctgatgc tctgcttcaa cgtcttcggc gatggcctgc
      661 gcgacgccct cgatcccaga atgcggaggt agggtggcga cacaaggcga aagcatcctc
      721 gaggtcaacg acctgcgtat ctccttctcg acctacgctg gggaggtgca ggcggtgcgc
      781 ggcgcttcgt tcgagctcag gcacggcgag accctcgcga ttgtcgggga gtcaggctcg
      841 ggcaaatcgg tggcggcaag gagcatcatg cgcctgaacc ccgaggcgaa caccatcgtc
      901 aggggtggcg agatcctctt cgagggagag gacctcctca agctcagtga gaagcagatg
      961 caggagatca ggggtcccaa gatcgcgatg gtcttccagg acccgatgac atcgctggac
     1021 ccgaccatga ggatagggcg ccagatctcc gagagcctga agaagcacct cgggctgtct
     1081 ggccgcgcgg cggcggagcg gtccgtggaa cttctcaaac tggtcggcat ccccaacccc
     1141 gagcaccgcc tcaagcagta cccgcaccag ttctccgggg gcatgcgcca gagggtggtc
     1201 atagccatag cgctctcctg cgacccccag atactcatag cggacgagcc gaccacggcc
     1261 ctcgacgtga caattcaggc ccagatcctc gaactcctgc gcgagttgca ggacaggctg
     1321 ggcatgtcca tcatcctgat cacgcacgac ctcggcgtgg tcgccagtac cgcccaccgc
     1381 atcggtgtga tgtacggcgg tatgatcgtg gagacgggta ccgtgcgcga tatcttctac
     1441 aacccccaga tgccctacac ctggggactt ctggcttcaa tccccctccc gacggccgac
     1501 cggagcctgg agctgatccc gatccccggc tcgccgccga gcgcgctcca tccgccgaag
     1561 ggttgcccct tcacggcccg ctgcccttac gccatgcgga tctgcgtcga tgagatgccc
     1621 gagtacacca cgttctccga cgagcacagg gcggcgtgct ggttgcacca ccagatggcc
     1681 ccaaacgtcg agcctccagc cagggtcagg gggacgagat gaccgaacag aacgggagcg
     1741 gcggggaaaa cgacaggccg ctgctggaga tcaaggacct caggaagtac ttcaaggtcg
     1801 gcagggagga cctgaaggct gtagacggga tctcgctcga catcaagaag ggcgagacgt
     1861 tcggcctcgt tggcgaatct ggttgcggca agtcaaccgc aggtagggtt cttgtcaggc
     1921 tctacgatcc ctccagcggc gaggttctgt acggcggcaa gaacatccac gaggcgacag
     1981 gcgacgaggg tagggtcctc aaccggaaga tgcagatgat cttccaggac ccccaggctt
     2041 ccttgaaccc gcgcatgatc gtcggcgaca ttatcgccga gggcatagac atacacggtc
     2101 tggtcgcaag caggaaggag cgcacggaac gtgtgcagga gctccttgat acggtaggcc
     2161 tgagcaagga gcacgcaagc cgctacccgc acgagttctc gggcggccag cgccagagga
     2221 tcggggtggc aagggcgctc gccgtcgacc ccgacttcat agtggccgac gagccggtga
     2281 gcgcacttga cgtctcgatc caggcccagg tcatcaacct gatgaagagg ctccagagag
     2341 agcgggacct gacgtacctg ttcatctccc acgacctctc ggtggtgaag tacatctccg
     2401 accg
//
LOCUS       DATAIR010000216         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_105785, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000216 DATAIR010000000
VERSION     DATAIR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105785"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1479)
                     /locus_tag="VHM69_05180"
     CDS             complement(106..1479)
                     /locus_tag="VHM69_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX2739818.1"
                     /translation="MGTFFKFSERGTNLRTEVVAGLTTFMTMAYIVFVNPAILGTEGT
                     GLPIPGVFFATCVAASVACIAMGLVANFPLALASGLGLNAIVAFTLILGQGLSWQEAM
                     AVIVVEGVLVTVLVLTNLREAVLNAIPPSLKFAIAVGIGLFIAFIGLKNAGLVVPNPD
                     TYVTLGDLTQGPVLLAAFGLIVTLALVARGMRGGILLGIVVTGIVGMIFGVVPPPEGI
                     VDFSFDTSTVGGGVLAVPQVLQISLLPVIFALFMTDFFDTMGTMFAVAQEGNLLDEEG
                     KVPQAKRILLVDSLAAVLGGAMGASSVTSYIESGSGVAEGGRTGMTSVVVGVLFLLAL
                     PFAPLISVFGGSLPIPNPMGGNEPLFVSPVTAPALIVVGFLIMTAVRFIPWERFDEAI
                     PAFLTILTLPLTFNISYGIGFGFISYVLIKLSRGKASEVHPLLYGTAVLFALAFVWPA
                     LQSLLSG"
     gene            complement(1683..2354)
                     /locus_tag="VHM69_05185"
     CDS             complement(1683..2354)
                     /locus_tag="VHM69_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739819.1"
                     /translation="MDTRDKRPLSKEEHPYEQRGLGPWIGRNPWLLMRLFTVIVFSFA
                     ASTVAANFSYGLEGEPTVLSVEQIRSGRLPSGTEVGDYVQVKGTPNVGDVSTNTIGRA
                     ESKIAVVSRYSTSYFYFPLGETGDNLLIQTTQGLPSGLESSGERVWRGKLETVGTVIF
                     HDTTQDGLKSAGLPRDESIPVVNTGETPEYLRKIFPAYSAIIVLWLLSVAWLAWKKNK
                     PLLGL"
     gene            2538..3161
                     /locus_tag="VHM69_05190"
     CDS             2538..3161
                     /locus_tag="VHM69_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739820.1"
                     /translation="MEEGREVQERDSWYTVVGVIDDGTGLNQAVKEIRDLGVGRDDLT
                     VVLKRVDSSEPEPFPEGTRYIVVPADRRGLEVPLGFAISFIVFGIFFAITTPAIGIPT
                     LLVFVSLAIILFAASLTRVGVNPILMEMEAPQEEADAWNEQFEHGKVLIFASTRERRL
                     LRPAREAMQRGGATYYIVDRRLEPRAVHQATLHRTGGRRVGESVLET"
     gene            3176..4525
                     /locus_tag="VHM69_05195"
     CDS             3176..4525
                     /locus_tag="VHM69_05195"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2739821.1"
                     /translation="MGVITTTSLHLLAQLNLTDAFSAVGGTRFIVGLTMLIHMFWAEI
                     FVGFALAAPVLQAWGARTGSPRMDRLAHSMARFNVLTFSTGATFAVLFIVLLVGLYPQ
                     VTTSLFTHFFYAIAIAMLSMILALWGMYSYYYKWDRYSVLNKKRHIALGFMMGFFIWI
                     WMVIMTGIDTYMVTGGPGVRPLSGSTIESFGTAIQGVFNPMFTELVLHRTFANLSWPA
                     FAVAAWASFMYIRSKTAEERAYYDWSSSVGLTWGVGFLMLQPIVGFTIVYALKLSQPE
                     SPVSGAASGGSFERLTSGETSNILLINLILVVVLIVLSNAAMYFGAERHPDHAGRVPI
                     RFFGLVAAVAGIYAISPLADFPYESPLFYLRYIALLVMVLATLGAFITYLRGRLRFRY
                     GSPGRTYRIVLMAAGVLAAVIALNMGLMKSNSRVPFTVYNQPGYTVEPGPPPTGFGR"
     gene            4561..5274
                     /locus_tag="VHM69_05200"
     CDS             4561..5274
                     /locus_tag="VHM69_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2739822.1"
                     /translation="MAELQKDPITRSDFIGFGLMGAIVGAILTIPPVAFVMSPVIKTN
                     VLGQSDIGSGWQEVGPVSEVPVEEPAVFEIEFPIEQIYGERSVQKQFPESQKSQTEFE
                     LKNAVWLSWKAPIKEQGRQGSSGDVIGKPERPAILDQKSEGFTESEREEILNQLNVLS
                     NSCAHLGCPVRWFPDQQLFLCPCHGGLYDINGGWVGGPPPRGMYRYTEAEVREDGKLY
                     VKHAYNIEPGLNKQHPYVV"
     gene            5284..6081
                     /locus_tag="VHM69_05205"
     CDS             5284..6081
                     /locus_tag="VHM69_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX2739823.1"
                     /translation="MIGRIRTQTTETSKFLVDWMEHRTGIVTMLEHFLYEPVPKRGAW
                     LYTLGSAVLFTITLQFLTGILLLFYYAPTTDDAWNSVYYIMNEAYFGQLIRGIHYWSA
                     NFLMAFIGLHMGQVFFSGGYKAPRELNWVVGVILLLLVIGLAFTGYLLRWDQDGFWAS
                     VVGMKIGSYSPFVGGYVINFLMGGDAVGPATLSRFFAIHVWLLPAAIAPLIGVHLYLL
                     RRHGVFGSEFEYTDRLAKLHERQRLEEYTRYEEDEEEPYVGDRADRE"
     gene            6156..6641
                     /locus_tag="VHM69_05210"
     CDS             6156..6641
                     /locus_tag="VHM69_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochromesubunit B of the bc complex-like
                     protein"
                     /protein_id="HEX2739824.1"
                     /translation="MYAPATGKAKPVEEEVITEENVFDRPDETIAFFPGQVLKDGYVS
                     TFILIACVVLSYAAPAPLTEPADTATVTYVPRPEWFFFFYEQMLMFFPGYALISFGAV
                     VVPTIFIGLLFAVPWLDRSPRHHNAVSRPFATVIGVLVVIVIVLNMLLAVSRIMNFPT
                     S"
     gene            6655..>7208
                     /locus_tag="VHM69_05215"
     CDS             6655..>7208
                     /locus_tag="VHM69_05215"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2739825.1"
                     /translation="MPIGNLDVPIIGKNVVIAVLVQSHILFATFIIGAVLIAATSEYL
                     GLVTKQINYERFARNLARFVVLLFASGAALAITFVLALVTLFPVFFSILQNIFFWVFL
                     VEAFMFLGQIIIVYAWYNVWDKLAYRKSLHVVFGFIAGFLGLMAMTMIDAVASFMLTP
                     AEVESQADLVARSFLNQTMVPLNM"
BASE COUNT         1380 a         2203 c         2224 g         1401 t
ORIGIN      
        1 gacacccgca agcgcaatat aacagatgcc gtacctgacg ctactatcgc ccgttttacc
       61 ctcgaagtgg cccggataaa ccccgaacgc gcataggttc ggggtttatc cggagagcag
      121 gctctgcaac gccggccaca cgaaggcgag ggcgaagagc acggcggtgc cgtagagcag
      181 cggatggacc tcactggcct tgccgcggga gagtttgatc aggacgtagg agataaagcc
      241 gaagccgatg ccgtaggaga tgttgaaggt cagaggcagc gtcaggatgg tcaggaacgc
      301 ggggatggcc tcgtcgaagc gctcccaagg gatgaacctt accgcggtca tgatcaggaa
      361 cccgaccacg atcagggcag gggcggtcac cggcgagacg aacagaggct cgttccctcc
      421 cataggattc ggtatcggga gcgagcctcc gaagaccgag atcaggggcg cgaacggcag
      481 ggcgagcagg aagaggacac cgacgactac gctggtcatg cctgtcctgc caccttctgc
      541 gacccccgac ccgctctcta tgtagctcgt caccgagctc gcacccatgg cgccacccag
      601 caccgccgcc agcgaatcga cgagcaatat acgcttggcc tgcggcacct tgccctcttc
      661 gtcgaggaga ttgccctcct gggcgacggc gaacatcgtc cccatcgtgt cgaagaagtc
      721 agtcatgaac agggcgaaga ttacgggcaa gagcgagatc tgcaagacct gcggcaccgc
      781 gagcaccccg cccccgacgg tcgaggtgtc gaagctgaag tccactatac cttcaggcgg
      841 aggcacgacc ccgaagatca tgccgacgat cccggtgacg acgatcccga gcaggatgcc
      901 gcccctcatg ccccgcgcaa ccagcgcgag ggtgactatg agcccgaacg ccgcgagcag
      961 tacaggaccc tgcgtgaggt ctcccagagt cacgtaggtg tcggggttcg gcacgacaag
     1021 cccagcattc ttgagcccga tgaacgcgat gaacagcccg atgccgacgg cgatggcgaa
     1081 cttcagagag ggggggatgg cgttgagcac ggcctcgcgc aaattggtca gcacgagaac
     1141 ggtgacgagc acgccttcca caacgatgac cgccatcgcc tcctgccagc tgagtccctg
     1201 gcccaggatg agagtgaagg ccactatcgc gttcaggccg agccccgagg cgagggcgag
     1261 cgggaagttc gcaaccaggc ccatcgcgat gcacgccacc gaggccgcga cgcacgtcgc
     1321 gaagaacacg cctggtatgg ggaggcccgt gccctcggta ccgaggatcg ccgggttcac
     1381 gaagactatg taggccatcg tcatgaaagt ggtcagcccg gcgaccacct ccgtcctgag
     1441 gttcgtcccc cgctcggaga acttgaagaa ggtgcccaag gtctagctct cccctctgca
     1501 gtctatatga acgagcaata tagcaactac atgctacagg tttcaggcat caggttgtgg
     1561 ccgtgcaaga cggggcgttg ctcttggtca cctctggcgg gtctttgtgg ctggtcgtcc
     1621 gaaatcgaga tatggaccgg ggccggctcg cgccgcgaac ggtactgcct gaagcctgaa
     1681 acctacaaac ctaaaagcgg cttgttcttc ttccaggcga gccaggccac agagaggagc
     1741 cagaggacga ttatggccga gtaggcgggg aagatctttc gcaggtactc tggcgtctcg
     1801 cccgtgttga cgaccggtat gctctcgtcg cgggggaggc ctgcgctctt gagcccgtcc
     1861 tgggtggtgt cgtggaagat gacggtcccc accgtctcga gcttgcccct ccagacgcgc
     1921 tcccccgagc tctcaagacc gcttgggagg ccctgggtgg tctggatcag gaggttgtcg
     1981 cccgtctccc cgagagggaa gtagaagtag ctcgtgctgt agcgggagac gaccgcgatt
     2041 ttcgattcgg ctcgcccgat cgtatttgta ctcacatcgc caacgttggg agtgcctttc
     2101 acttgcacgt agtccccgac ctccgtgccg gacggcagcc tgcctgaccg tatctgctcg
     2161 acgctcagga cggtgggctc gccttcgagg ccataggaga aattggcggc taccgtcgag
     2221 gctgcgaaag agaagacgat gacggtaaac agccgcatga gcaaccacgg gtttcgcccg
     2281 atccagggcc ccagccccct ctgttcgtag ggatgttctt cctttgagag tggtctcttg
     2341 tctctggtgt ccacgcacgc agtgtacccg agcgcgcggg gcgtgatgag taaggcttca
     2401 catatcacgg attgtgaaag agattcccgc gtgaggtggg gaacggtcta gtatgcaggg
     2461 actcgacaat gtatgctgtt gggcggctga aaccgccgcg cacggcaggc tcgtatccct
     2521 ctctggaggt tggagttttg gaggaagggc gggaggtaca ggagcgggac tcctggtaca
     2581 ccgtagtagg ggtaatagat gacgggacgg ggctcaacca ggccgtcaag gagatcaggg
     2641 acctcggcgt gggcagggac gacctgacgg tcgtcctcaa acgcgtggat tcaagcgagc
     2701 ctgagccttt ccctgaaggc acgcgctaca tcgttgtgcc ggcggacagg cgcgggctcg
     2761 aggtgccttt gggcttcgcg atatccttca tagtcttcgg catcttcttc gccatcacga
     2821 caccagcgat cgggataccg accctcttgg tcttcgtctc gctggcgatc atactcttcg
     2881 ccgcctcgct cacgagggtc ggggtcaacc ctatcctcat ggagatggag gcgccacagg
     2941 aggaggcgga cgcctggaac gagcagttcg agcacgggaa ggtcctgatc ttcgcctcta
     3001 cgcgcgagcg gcgactgctg cgtcctgcac gcgaggccat gcagcgcggc ggcgcgacgt
     3061 actacatagt tgaccggcgg ctggagccca gggccgtcca ccaggccacc ctgcaccgca
     3121 ctggaggacg cagggtgggc gaatcggtcc tggaaaccta gaaggagtcg tgagcgtggg
     3181 cgtgataact actacgagcc tgcacctcct ggctcagttg aaccttactg acgccttctc
     3241 ggccgttggc ggcacccgat tcatcgtcgg actcacgatg ctaattcaca tgttctgggc
     3301 ggagatattc gttgggttcg ccctcgccgc gcctgtgttg caggcctggg gggcacgaac
     3361 ggggagccca cggatggacc gtctcgccca ctccatggcc cgcttcaacg tgctcacctt
     3421 ctcgacaggg gcgaccttcg cggtcctctt tatcgtactg ctcgtcggcc tttatcctca
     3481 ggtgacgacg tcgctcttca cgcacttctt ctacgctatc gccatcgcga tgctctccat
     3541 gatcctcgcg ctctggggga tgtattccta ctactacaag tgggaccgtt acagcgtgct
     3601 caacaagaag cggcacatag cgctcgggtt catgatgggc ttcttcatct ggatctggat
     3661 ggtcatcatg accggcatag atacctacat ggtcaccgga ggaccaggcg tcaggccact
     3721 ctcgggctct accatcgaga gcttcggcac ggcgatacag ggtgtcttca acccgatgtt
     3781 cacggagctg gtgttgcaca ggaccttcgc caacctctcg tggcccgcct tcgccgtcgc
     3841 cgcgtgggca tcgtttatgt acatccggtc gaagacggcc gaggagaggg cttactacga
     3901 ctggagcagc tcggttggcc ttacgtgggg cgtcgggttc ctcatgcttc agccgatcgt
     3961 aggcttcacg atagtctacg ctttgaaact ctcccagcct gaaagccctg tgtccggcgc
     4021 ggcctcgggg ggctcattcg agcgactcac ctcgggggag acatcgaaca ttctcttgat
     4081 aaacctgatc cttgtcgtcg tactcatcgt gctctccaac gcggccatgt atttcggggc
     4141 ggagaggcac cctgaccatg cagggcgcgt gccgatccgg ttcttcgggc tcgtggccgc
     4201 cgtggccggg atctacgcga tctcaccgct cgctgatttc ccctacgagt cgccgctctt
     4261 ctacctgcgc tacatagcgc tgctcgtgat ggtgctcgcc accctcgggg cgttcataac
     4321 gtatttgcgc ggcaggctgc gtttcaggta cggcagtccc ggcaggactt acaggatcgt
     4381 gctgatggca gcgggggttc tcgcggcggt gatcgcgctc aacatggggc tcatgaagtc
     4441 caattcgagg gtccctttca cggtatataa tcagcctggc tacacggtgg aaccaggccc
     4501 cccgccgacg ggattcggca ggtgagcttc gagtctctgg gactctagaa ggggcgaagc
     4561 gtggcggagc tacagaagga tcccataacc aggagcgact tcatcggctt cggcctgatg
     4621 ggggcgatag tgggggcgat tctgaccatc ccccccgtcg ccttcgtgat gagcccggtc
     4681 ataaagacga acgtgctcgg ccaatcagac atagggtccg gttggcagga ggtaggcccc
     4741 gtctccgagg tccccgtaga ggagcccgcg gtcttcgaga tcgaattccc catagagcag
     4801 atctacgggg aacggtcagt acagaagcag ttccccgaat cccagaagtc tcagactgag
     4861 ttcgagctga agaacgccgt ctggctctca tggaaggcgc caataaaaga gcagggcagg
     4921 cagggatcga gcggagacgt gatcggtaaa cccgagcgcc ccgcgatcct tgaccagaag
     4981 agcgaagggt tcacggagtc cgagcgcgag gagatcctga accagctcaa cgtgctctcc
     5041 aactcctgcg cccacctcgg ctgccccgtg aggtggttcc ctgatcagca actcttcctt
     5101 tgcccttgcc acggtgggct ctacgacatc aacggcgggt gggtcggagg gccgccgccg
     5161 cgcgggatgt accgctacac cgaggcggag gtcagggagg acggcaaact ctacgtcaag
     5221 cacgcgtaca acatcgagcc cggcctcaac aagcaacacc cctacgtggt ctaggagggt
     5281 agcatgatcg gcaggataag gacccagacg acggagacaa gcaagttcct ggtcgactgg
     5341 atggagcacc ggactgggat agtcacgatg ctcgaacact tcctctacga gcccgtgccc
     5401 aaacgcggcg cctggctcta caccctgggc agcgccgtgc tcttcaccat aacgttgcag
     5461 ttcctgacag ggatcctcct tctcttctac tacgcgccga ccaccgacga cgcctggaac
     5521 tcggtctact acatcatgaa cgaggcgtac ttcgggcagc tcatccgcgg catccactac
     5581 tggtcggcga acttcctgat ggcgtttatc gggttgcaca tgggccaggt gttcttctcg
     5641 ggcggctaca aggcgccgcg agagctcaac tgggtcgtcg gggtcatcct gctcctgctc
     5701 gtcatcgggc tcgcgtttac ggggtacctg ctgcgctggg accaggacgg tttctgggcc
     5761 tccgtggtgg ggatgaagat cgggtcttac tcgccgtttg tcggggggta cgtgatcaac
     5821 ttcctcatgg gcggcgacgc tgtcgggcct gccacgttgt cgcgcttttt cgccatacac
     5881 gtctggcttc tgcccgccgc gatagcgccg ttgatcggcg tgcacctgta cctgctcagg
     5941 cgtcacgggg tcttcggcag cgagttcgag tacacggacc gcctcgccaa gctgcacgag
     6001 cgccagcgcc tcgaagagta cacccgctac gaggaggacg aagaggagcc ttacgtggga
     6061 gatcgcgcag acagggagta acagctttta ccgagaggac ggacgtacca ctggtacgat
     6121 tggggcacag aacgtaatag agagaggtgt tgaaagtgta cgctccggcg actggcaagg
     6181 ccaaaccggt tgaagaagag gtaatcacgg aagagaacgt gttcgatcgt cctgacgaga
     6241 ccatagcgtt cttccccggt caggtgctca aggacggtta cgtaagcacc ttcatcctca
     6301 tcgcctgcgt cgtgctctcg tacgccgccc cggcgcctct aaccgagccc gcggacacgg
     6361 cgacggtcac ctacgtgcca aggccagagt ggttcttctt cttctacgag cagatgctca
     6421 tgttcttccc cggatacgcc ctgatctcct tcggggccgt cgtcgtgccg acgatcttca
     6481 tagggctgct cttcgcggta ccctggctcg acaggagccc gcggcaccac aacgccgtca
     6541 gcaggccctt cgccactgtc ataggcgtcc ttgtggtcat agtcatcgtc ctcaacatgc
     6601 tgctggctgt cagcaggatc atgaacttcc caacgagcta gaggagaccg agcggtgccc
     6661 ataggcaacc ttgatgtacc gataatcggc aagaacgtcg tcatcgccgt tcttgtccag
     6721 tcgcacatcc tctttgcgac cttcattatc ggggccgtcc tgatagcggc cacgagcgag
     6781 tacctcgggc tggtgaccaa gcagatcaac tacgagcgct tcgccaggaa cctggcgcgc
     6841 ttcgtcgtgc tccttttcgc ctcgggcgcc gcactggcga tcaccttcgt gctcgccctc
     6901 gtgacgctgt ttccggtctt cttctcgatc ctgcagaaca tcttcttctg ggttttcctg
     6961 gtcgaggcct tcatgttcct cggccagatc atcatcgtct acgcctggta caacgtgtgg
     7021 gacaagctcg cctaccgcaa gagcctgcac gtcgtcttcg ggttcatagc gggcttcttg
     7081 gggttgatgg cgatgacaat gatcgacgcc gtggcctctt tcatgctcac gcccgccgag
     7141 gtcgagtccc aggccgacct cgtcgcccgc agcttcctga accagacgat ggtgccgctc
     7201 aacatgca
//
LOCUS       DATAIR010000217         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_13640, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000217 DATAIR010000000
VERSION     DATAIR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13640"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VHM69_05220"
     CDS             <1..709
                     /locus_tag="VHM69_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HEX2739826.1"
                     /translation="LGLLWYPFLRRLGTAGMNFVLALTVGLLVFLVLDTLLEAVEIAG
                     ELPGAFSGVPMVILVALLTLLGLLGVERLFRRGREEASRLATSYRIAFGIGLHNLGEG
                     LAIGAAFALGEAALGTFLILGFTLHNITEGVGIAAPVLREKRPNFAHFAALALLGGGP
                     AIVGTWIGGFAYSNLLSALFLAVGAGAILQVVYEVGRLLLRDSARSKTPTLSGTNLGG
                     LTAGRAIMYVTALLVSV"
     gene            910..>1875
                     /locus_tag="VHM69_05225"
     CDS             910..>1875
                     /locus_tag="VHM69_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2739827.1"
                     /translation="MKDRQEDYFEEIEGVLPGETATARAAHESLKGDRRGLRRLWPFL
                     GPAFIAAVAYVDPGNFATNISGGAQFGYLLLWVILTSNLMAMLIQSMSAKLGIATGKN
                     LPEVCRERFPRLVTLGLWVQAEVIAMATDLAEFIGAALALNLLFGIPLFPAGLLTAVG
                     AFGILELQRRGFRPLEAVIAAMVGLIVVAFAFQMFYAEPEADRILAGLFTPQFAGTES
                     VLLAAGILGATVMPHVIYLHSALTQHRVVGRTEEEKKKIFRFELVDVVIAMAIAGTIN
                     ASMLIMAAALFHSNGLTGVADIDKAFEQLRILEGNTASILFGVALL"
BASE COUNT          319 a          566 c          603 g          387 t
ORIGIN      
        1 gcttgggctt ctgtggtacc cgttcctccg gcgcctcggc accgctggca tgaacttcgt
       61 cctcgctctg acggtcgggt tgctggtctt cctggtgctc gacacgctgc tcgaggccgt
      121 ggagatcgcc ggtgagctgc cgggagcttt ctcaggggtc ccgatggtga ttctcgttgc
      181 cctcttgacg ctgctcgggc tcctcggtgt cgagcgactc ttccggcgcg ggcgtgagga
      241 ggcgagtcgg ctcgcgacct cgtaccggat agcctttggg atagggttgc ataacctggg
      301 tgagggtctc gcaatagggg cggcgttcgc gctaggcgag gcggcactgg ggaccttctt
      361 gatcctcggc ttcaccctcc acaacatcac cgagggagtc ggcatcgccg cgccggtact
      421 gagggagaag cggccgaact tcgcgcactt cgccgcactc gcgctcctcg gagggggacc
      481 ggccatcgtc ggaacctgga tcgggggctt cgcttactcg aacctcctct ccgccctctt
      541 cctcgctgta ggagccgggg ccatactcca ggtcgtctac gaggtgggac gcctcctcct
      601 gagagactcg gcccgctcca agacgcccac cctctcgggc acgaacctcg gagggctcac
      661 cgccggaaga gcgatcatgt acgtgacggc cctgctggtg agcgtgtagg ccgccagaga
      721 gggaggttca tcctctctcc tgcacagaac ggcaaaatac gcaggctgga gacggtatag
      781 gtccgtccgc gagcgcgcct gaaaacgcaa ctctgtgcgt gagagcggct atgaactttc
      841 tttaggtctg gtagactccg cgattagcca aggctaaaaa tatcccaaaa cgatagtgca
      901 gaggtatatg tgaaggaccg tcaagaagac tattttgagg agatcgaagg cgtactccct
      961 ggagagactg cgacggcgcg ggcggcgcac gaatcgctca agggagaccg caggggcttg
     1021 cgcaggctct ggccgttcct cgggcctgcg ttcattgcgg cggtggccta cgttgaccca
     1081 ggcaacttcg cgacgaacat ctcgggtgga gcgcagttcg ggtatctgct gttgtgggtg
     1141 atcctgacct ccaacctgat ggctatgttg atacagtcca tgtcggcgaa actcgggatc
     1201 gccaccggaa agaacctgcc cgaggtgtgt cgcgagcggt tcccgaggct ggttacgctg
     1261 gggctctggg tacaggcaga ggtcatcgcg atggcgacgg atctcgcgga attcatcggc
     1321 gcggccctgg cgctaaattt gcttttcgga ataccactct ttcctgctgg gcttctgacg
     1381 gcggtcgggg cgttcggcat actggagtta cagcgccggg gcttcaggcc gctcgaggcg
     1441 gtcatcgccg cgatggtcgg cttgatcgtg gttgctttcg ccttccagat gttctacgcc
     1501 gagcctgagg cggacaggat actcgccggt ctcttcacgc cgcagttcgc tggaacagag
     1561 agcgttcttc tggccgcagg catcctcggg gcaaccgtca tgccgcacgt catctacctc
     1621 cactcggcgc tcacgcagca tcgggtggtc gggaggacgg aggaggagaa aaagaagatc
     1681 ttccgcttcg agcttgtcga cgtggtcatc gcgatggcca tagcaggtac catcaacgcc
     1741 tcgatgctca tcatggccgc cgcactcttc cactcgaatg gcctgaccgg cgtcgccgat
     1801 atagacaagg ccttcgagca gttgcgtatt ctcgaaggca acaccgcctc catcctcttc
     1861 ggggtggccc ttctc
//
LOCUS       DATAIR010000218         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_52331, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000218 DATAIR010000000
VERSION     DATAIR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52331"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="aceA"
                     /locus_tag="VHM69_05230"
     CDS             complement(<1..665)
                     /gene="aceA"
                     /locus_tag="VHM69_05230"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX2739828.1"
                     /translation="MENGKNGARRIEQEWEGERWEGIERPYSADDVARLRGSIVIEHT
                     LARLGAERLWRLMHERPFVRSLGALTGNQAVQQVKAGLEAIYLSGWQVAADANLAGHM
                     YPDQSLYPANSVPHVVKRINQALQRADQIHHSEGRNGINWFAPIVADAEAGFGGALNV
                     FELMKAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTSQFVRTLTAARLASDVLEVP
                     TVI"
     gene            complement(880..2484)
                     /gene="aceB"
                     /locus_tag="VHM69_05235"
     CDS             complement(880..2484)
                     /gene="aceB"
                     /locus_tag="VHM69_05235"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX2739829.1"
                     /translation="MADTTRTYAEGIEFTAEIPDEFAQILTPEAVAFVATLSREFRGR
                     VDEILAKRAERQERISAGEMPDFLPETREIREGDWKIAPIPDDLQDRRVELTGPPDRK
                     MTINALNSGASCWMADFEDANCPTWNNMLESQLNMKEAIERTISFDDPKSGKHYELHE
                     DVAVILARPRGWHLFEKHMLVDGKQVPGGLFDFGLYFFHNAQTLLDNGSGPYFYLPKM
                     ESHLDARLWNDVFNLAQDGLGIPRGTIKATVLIETILATFEMHEILYELRDHSAGLNC
                     GRWDYIFSYIKKFREHDMLLPDRNSVTMTVPFMRAYSLLTIKTCHQRGAHAMGGMAAQ
                     IPRSDDPEWTEFANNKVREDKEREAKDGHDGTWVAHPGMVGLAKEAFDEYMPGPNQLD
                     KIPDVNPSAADLLEKPEGPITMDGFRNNISVGVQYLAAWLAGRGAVPVFNLMEDAATA
                     EISRAQVWQWIHHPKGILEDGTEVTEDLFNQVLDEELEKIKTDIVGAERWEQDEFGKA
                     AELFARISTDDEFVEFLTLPGYEYLD"
     gene            complement(2568..2777)
                     /locus_tag="VHM69_05240"
     CDS             complement(2568..2777)
                     /locus_tag="VHM69_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739830.1"
                     /translation="MIPVQYRDPQTEEILERRYEESAPSIGTRVMIGFDEFQVLYRWR
                     CVPTSCIVYVHRVVREIRQEARSAA"
     gene            2887..3849
                     /locus_tag="VHM69_05245"
     CDS             2887..3849
                     /locus_tag="VHM69_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2739831.1"
                     /translation="MVLFRQLECFLAVERLGNLSRAAEEMFLTQPTLTARVKALEEEL
                     GDALFVRTSRGMRLTEAGREFLPYAERIVGSFEEGKRRLAEFRGASGGRLRIGASPGV
                     GTYALPGLLERFAAAHPRVSISVRTGHSEAILEMTLKEEVQLGLTRAMRHPEIESLPL
                     YEDELVLIVDPGHRFTRKGTASLAEIGEEQLIFFDHDSSYFEQTHALFRNAGIRELRT
                     MEVDNIEAAKRMVEHRLGVAFLPRTAVVRSVSTGNLVLVSIEENPEMSRSIVALKRRD
                     VPSTGPVAAFLEVADSMGEKRDSTQLSPALATEFENLQLIDLFT"
     gene            complement(3950..>4789)
                     /locus_tag="VHM69_05250"
     CDS             complement(3950..>4789)
                     /locus_tag="VHM69_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739832.1"
                     /translation="VKRRIPIIAIVAAFAMLIASGCNANDGANAGSDQSQGGAGAGSP
                     RDLPQGAEPVDLDPKNFTTRIDNPYWPMDPGTRWTYRETDEEGAELKVVVTVSDQTKK
                     IANGVTARVVRDTVTEDGEIVEDTFDWYAQDKAGNIWYLGEDTAEFENGKLKTKEGSF
                     EAGVDGAQPGIIMPADPRDGMQYRQEYYNGEAEDNGEVLSTDEMAQVPFGQYDNALLT
                     KDTITIEPNVLEYKLYAREVGPVLTLGVSGGPGSREELINLDTASEKETEEAGTAPLG
                     SAS"
BASE COUNT          808 a         1522 c         1452 g         1007 t
ORIGIN      
        1 atgatcaccg tcggcacctc gagcacgtcg gaggccaggc gcgccgccgt cagcgtgcgg
       61 acgaactgcg aggtcggcac cagcaccttg ccgcccaggt ggccgcactt cttctcggag
      121 gcgagctggt cctcgaagtg gacgccagcc gctccggcct cgatcatggc cttcatcaac
      181 tcgaagacat tgagcgcgcc accgaaaccg gcctccgcgt cggcgactat cggggcgaac
      241 cagttgatgc cgttcctgcc ttcggagtgg tggatctggt ccgccctctg caacgcctgg
      301 ttgatcctct tgacgacatg cggcacgctg ttggccggat acaggctctg gtcaggatac
      361 atgtgccctg ccagattggc gtcagcagcc acctgccagc cggagaggta gatcgcctcc
      421 agcccagcct tgacctgctg cacagcctga tttcccgtca atgccccgag agaccgcacg
      481 aatggccgct cgtgcatcag cctccacaaa cgctcggcac caagccgcgc cagcgtgtgc
      541 tcgatcacga tcgagccccg caaccgcgca acgtcatcag ccgaatacgg ccgctcgatg
      601 ccctcccagc gctcgccttc ccactcctgc tctatccgcc tggcgccgtt cttcccgttc
      661 tccaccactt cctccttcag tcttcgatcc tgcatctcta gcttacatta tgcacttata
      721 tgttcacata tgtgcaaagg aggcgcgaaa agcgcctgtc tccgtggcat gaggcgtggg
      781 tacgtgaccc acgctccggg gcaggaggac ggggaccgtc ggtcccgtga cggcccctgt
      841 tctgctcgcc tggacgatgt ctaggatact ttctcctctc tagtccaggt actcgtaacc
      901 tgggagggtc aggaactcga cgaactcatc gtcggtcgag atacgggcga agagttctgc
      961 ggccttgccg aactcgtcct gttcccagcg ttcggcgccg acgatgtcgg ttttgatctt
     1021 ctccagttcc tcatcgagga cctggttgaa gaggtcttcc gtcacctcgg taccgtcctc
     1081 caggatacct ttggggtggt ggatccactg ccacacctgg gcgcggctga tctccgccgt
     1141 cgccgcgtcc tccatgaggt tgaagacggg gacagcgccg cgaccggcga gccacgccgc
     1201 gagatactgc acgcccacgg agatgttgtt gcggaagccg tccatggtga tgggaccctc
     1261 gggcttctcc agcaggtccg ccgctgacgg gttcacgtcg gggatcttgt cgagctggtt
     1321 cggaccaggc atgtactcgt cgaaggcttc tttggcgagc ccgaccatgc ctggatgggc
     1381 cacccaggtc ccgtcgtggc cgtccttcgc ttcgcgctcc ttgtcctcgc gcaccttgtt
     1441 gttggcgaac tcggtccact cggggtcgtc gctgcgtggg atctgggccg ccatcccccc
     1501 catcgcgtgc gcaccgcgct gatggcaggt cttgatagtg agcagcgaat acgcgcgcat
     1561 gaacgggacg gtcatcgtca ccgagttgcg gtcggggagg agcatgtcgt gctcgcggaa
     1621 cttcttgatg taggagaaga tgtagtccca cctgccgcag ttcagcccgg cggaatggtc
     1681 gcgcaactcg tagaggatct cgtgcatctc gaacgtcgcg agtatcgtct caatgaggac
     1741 cgtcgccttt atggtccccc tcggtatacc gagcccgtcc tgcgccagat tgaagacgtc
     1801 gttccagagc ctggcatcca ggtggctctc catcttgggt agatagaagt acggacctga
     1861 accgttgtcc aggagcgtct gcgcgttgtg gaagaagtac agtccgaagt cgaagaggcc
     1921 gccggggacc tgcttaccgt caacgagcat gtgcttctcg aagaggtgcc agccgcgggg
     1981 ccgggccagg atgacggcca cgtcctcgtg cagctcgtag tgcttgcccg acttcgggtc
     2041 atcgaagctg atggtgcgct cgatggcctc tttcatgttc agctggctct ccagcatgtt
     2101 gttccacgtc gggcagttgg cgtcctcgaa gtccgccatc cagcagctcg cccccgagtt
     2161 gagagcgttt atggtcatct tgcggtcggg aggaccggtc agctcgactc tacggtcttg
     2221 aaggtcgtcg gggatcggcg ctatcttcca gtcgccctcc cggatctcac gcgtctcagg
     2281 caggaagtcg ggcatctcgc cagcgctgat cctctcctgg cgctcggccc gcttcgcgag
     2341 gatctcatcg acccttcctc tgaactcccg tgagagcgtc gccacgaaag cgaccgcctc
     2401 cggcgtaagt atctgcgcga actcgtcggg gatctccgcc gtaaactcga tcccctcggc
     2461 atacgtgcgc gtggtatcag ccataacctc cggctcctct ctacagcttc cagagcgagg
     2521 ctactctagc aagccacgcc tccggccgct tggacccgga ggccgcacta agccgcggac
     2581 ctggcctcct gacgtatctc cctgacgacc ctgtggacgt aaacgatgca cgaggtcggc
     2641 acgcatcgcc acctgtagag gacctgaaac tcgtcgaaac ctatcatcac ccgcgtcccg
     2701 atcgaaggcg cgctctcctc gtagcggcgc tccaggatct cctccgtctg cgggtctctg
     2761 tactgtaccg ggatcatagt ttctcctctc aacttcttcg tcgtgaaagc aagataccaa
     2821 tacccttgat atttatccaa tcgtttgctt gaattatctc gatagagaaa atctatatcg
     2881 gaggatatgg tgctctttcg gcagttggaa tgcttcctgg cggtggagag gttagggaac
     2941 ctgagtcggg ccgctgagga gatgttcctg acgcaaccga cgttgacggc gcgagtaaaa
     3001 gcgctagagg aagagcttgg ggacgcgctc ttcgtgcgca cgagccgtgg gatgaggctc
     3061 acggaggctg gcagggagtt tctaccgtat gcggagagga tcgtcggcag tttcgaggaa
     3121 ggcaagcgcc ggttagccga gttcagaggg gcttccgggg ggcggctcag gatcggggct
     3181 tcgcccggcg ttgggactta tgcgttgccc gggctattgg agaggttcgc ggcagcgcat
     3241 ccgagggttt cgatctccgt taggacgggg cattccgagg cgatcctcga gatgacactt
     3301 aaagaagagg tgcagctcgg cctcacgcgg gccatgcgcc atcccgagat agaaagtctg
     3361 cccctctacg aggatgagct ggtgctcatc gtggatcctg ggcacaggtt cacgaggaag
     3421 ggtacggcga gcctcgcgga gatcggggaa gagcagctca tcttcttcga ccacgactcg
     3481 agctatttcg agcagaccca tgcgctgttc aggaacgccg gcatcaggga gctgaggaca
     3541 atggaggtcg acaacatcga ggccgccaag aggatggtcg agcaccgtct cggagtggct
     3601 ttcttgcccc ggacagccgt ggtcaggtcg gtctcgacag gcaacctcgt cctcgtctcg
     3661 atagaggaga accccgagat gagccgctcg atagtcgccc tcaaacgtcg agatgttccg
     3721 tcaacaggcc ccgtagccgc cttcctcgaa gtcgcggact ccatgggcga gaagcgcgac
     3781 agcacccagc tctccccagc cctcgcgacc gagttcgaga acctgcagct catagacctg
     3841 ttcacctgag gggaaccgtc ggagttcggt tttggcctct tgcgacgcag taccgtgcgg
     3901 ccatgcagat catcccttct cctcgacggg cacccatcgt ccgtcacgct tacgaagcgg
     3961 aacctagcgg cgccgtgccg gcctcttccg tctccttctc gctggccgta tcgaggttga
     4021 tgagctcctc gcggctgcct ggaccgcccg agactccaag ggtgagaaca ggtccgactt
     4081 ccctggcgta cagcttgtat tcgagtacgt tcggctcgat cgtgatcgtg tccttcgtca
     4141 ggagcgcgtt gtcgtactgt ccgaagggta cctgggccat ctcgtcggtg ctgaggacct
     4201 cgccgttatc ctcggcctca ccattgtagt attcctgtcg gtactgcatg ccgtccctgg
     4261 gatcagccgg catgatgatg ccaggctggg cgccgtcgac cccggcctcg aaggagcctt
     4321 ccttcgtctt gagcttgccg ttctcgaact cggcggtgtc ctcgcccagg taccagatgt
     4381 tgccagcttt gtcctgggcg taccagtcga aggtgtcctc gacgatctct ccgtcctccg
     4441 tgaccgtgtc gcgcacgacc ctggcggtga caccgtttgc gatcttcttc gtctggtcgc
     4501 tcaccgttac gacgaccttt agctcggcgc cctcctcgtc ggtctcgcgg tatgtccatc
     4561 gcgtgccagg gtccatcggc cagtatgggt tgtcgatcct ggtggtaaag ttcttcgggt
     4621 cgaggtccac gggttcggcc ccctgcggca ggtcgcgtgg gctcccggct cccgcaccgc
     4681 cctgcgactg atccgagccg gcattcgcgc cgtcattcgc gttgcaaccg ctggcgatga
     4741 gcatggcgaa cgcggccacg atggcgatga ttgggatgcg gcgcttgac
//
LOCUS       DATAIR010000219         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_290885, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000219 DATAIR010000000
VERSION     DATAIR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290885"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..868
                     /gene="raiA"
                     /locus_tag="VHM69_05255"
     CDS             296..868
                     /gene="raiA"
                     /locus_tag="VHM69_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX2739833.1"
                     /translation="MDILIKGRNISVTEALERYAWEKVERVSRFFDDERSASRAEVEL
                     IHERNPSIPEAEVAEATLFINGSVLKAKEASEDMYASIDRMSDKLERQVKRFRGRQID
                     RWQGQLKNSSDEAPDAAQPFVVEEEEEIETRIVRTKQFQMKPMSAEEAVLQLELLDHD
                     FYVFTSADTGDINVVYRRRDGDYGLIEPAR"
     gene            902..3493
                     /gene="secA"
                     /locus_tag="VHM69_05260"
     CDS             902..3493
                     /gene="secA"
                     /locus_tag="VHM69_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX2739834.1"
                     /translation="MANLLTKILRMGEGRKVKVLQQRVEAVSALEPEMEKLRDEELRA
                     KTDEFRGRLAGGETLDDLMPEAFAVVREVSRRTLKMRPFDEQIMGGIALHEGKIAEMK
                     TGEGKTLAATMPVYLNALEGKGVHVVTVNDYLARRDAGWMGEIYEFLGLEVGLVQESM
                     DFEERKVAYHSDITYGTNAQFGFDYLRDNIATSVDQLVQPELNFAIVDEVDSILVDEA
                     RTPLIISGMPESAADVYYRFAAIVPRLKAGEDYEVDEKKQQVAPTESGVDKVEKALGL
                     ENLYDDVNTNLVNHLNQALRAQTLFHKDDEYIVQDGQVYIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVEIKEENQTVATITIQNFFRMYDKLSGMTGTAATEADEFMHTYKMEVVS
                     IPTHKEMIRDDKDDLVYKSEKSKHRAVVEDIVERHKKGQPVLVGTVSVDVSERLAGLL
                     KRRGIRHNVLNAKQHEREAEIIAEAGERGAVTIATNMAGRGTDIKLGEGVSELGGLYV
                     LGTERHEARRIDNQLRGRSGRQGDPGESRFYLSFEDELLRLFGGQKMQGIIDRIGLEE
                     DVPIEAGMISNSVRRAQEQVESRNFQIRKRILEYDDVLNKQREVIYTIRRDILMGEDV
                     DTMSYVEDVLTEAVSEHVSADVYPEDWDLDALTADVNRFYPSRVDLKSLDVERLTATE
                     VLAMVLEDARERLDERKAEWDERTAELEKRGLARGDGVATFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKEDYVTYIYRVENIKL
                     REEEMRQLSYSGGEDPSQSTRSPRQRSSSVKVGRNEPCPCGSGKKYKKCHGMPGAPPL
                     GSEARAG"
     gene            3493..4584
                     /gene="prfB"
                     /locus_tag="VHM69_05265"
     CDS             3493..4584
                     /gene="prfB"
                     /locus_tag="VHM69_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX2739835.1"
                     /translation="MTELDDRRSELEGRLAELEAFFGVEGLRAEAEGLGEEMSRPGFW
                     DDPEEAKTVSARFSRVQGRIELLDGLRERLSDAGELLELAEEDGELLREVGEELRRVA
                     RVIEEQEVARLFAGEYDDGDAILTINSGAGGVDSQDWAEMLARMYRRWVERRGFGLEV
                     IEYTEGEEAGIKSATFTVSGEYAFGLLSAERGVHRLVRISPFDAGARRHTSFASVAVA
                     PVVGEAVEVQIEEKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQH
                     QNREVAMRVLRARLFELEREKREREIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRT
                     GEETANVDRVLDGDIDAFIYAYLKNRTAA"
BASE COUNT         1000 a         1354 c         1593 g          746 t
ORIGIN      
        1 cgacgttttc accactggag caacgatgag cgcgtgcgcc gcaacactcg tcgaagcagg
       61 agcgagagag gtccacgcgc tgagcctgtg caggacatgt taggtttcag ctttcagcca
      121 tcagcagtca gcaagagagt gtggcttgac actccgtggc caaccctcga acctcttgct
      181 gaagcggtcc tgcgcatgga gtgcatcgcg gcaccactta gttgcggatc ggatggaaca
      241 gctgatagct gagtgctgat agctgacggc taaagccgac cggagggagg ctacattgga
      301 catactcatc aagggacgca acatctcggt gacggaggcg ctcgagcggt acgcctggga
      361 gaaggtggag cgggtctccc ggttcttcga cgacgagagg agcgcctcgc gggccgaggt
      421 cgagctcatc cacgagcgga acccatctat cccagaggct gaggttgcag aggcgacgct
      481 gttcatcaac gggtctgtgc tgaaggctaa ggaggcgtcc gaggacatgt acgcctccat
      541 cgacaggatg tcggacaagc tggagcggca ggtcaagcga ttcagggggc gtcagatcga
      601 ccgctggcag ggccagctca agaactcctc cgacgaggct ccagacgcgg cgcagccctt
      661 cgtcgtcgag gaagaagagg agatcgagac gcgcatagtc aggaccaagc agttccagat
      721 gaaaccgatg agcgcggagg aggcggtcct ccagcttgag ctgctcgacc acgacttcta
      781 cgtgttcacg agcgccgata ccggcgacat aaatgtggta taccggcgtc gtgacggaga
      841 ctacgggctc attgagcctg cccgttagtc cggtgcgacc acaactatga ggtagcttgt
      901 cttggcgaac ttgttaacaa agatactgag gatgggcgag ggccgcaagg tcaaggtcct
      961 tcagcaacgc gtggaggctg tctcggctct ggagccggag atggagaagc tacgcgatga
     1021 ggagctgcgg gcgaagaccg acgagtttcg cgggaggctc gcaggggggg agacgctcga
     1081 cgaccttatg cccgaggcct tcgctgtggt gcgtgaggtc tccaggagaa cgctcaagat
     1141 gcgtcccttc gacgagcaga tcatgggcgg tatcgctttg cacgagggta agatcgcgga
     1201 gatgaagacc ggcgagggca agacgcttgc cgccaccatg cccgtctacc tcaacgctct
     1261 cgaaggcaag ggcgttcacg tcgtcacggt caacgactat ctggcacgtc gcgacgcagg
     1321 gtggatgggg gagatctacg agttcctcgg gctcgaggtc ggtttggtgc aggagtctat
     1381 ggacttcgaa gagcgcaagg tcgcctacca ctccgacata acctacggca cgaacgctca
     1441 attcggcttc gattacctca gggacaacat cgcgacgtcc gtagatcagc tcgtgcagcc
     1501 cgaactcaac ttcgccattg tggacgaggt ggactcgatc cttgtggacg aggcgcgcac
     1561 cccgctcatc atctcgggta tgccggagag cgccgccgac gtctactatc gcttcgccgc
     1621 catagttccc aggctcaaag ccggcgagga ctacgaggtc gacgagaaga agcagcaggt
     1681 agcaccgacc gagagtggcg tcgataaggt ggagaaggcg ctcggccttg agaacctata
     1741 cgacgacgtc aacaccaacc tcgtcaacca cctcaaccag gctttgcggg cgcagacgct
     1801 cttccacaaa gacgacgagt acatcgtcca ggacgggcag gtttacattg tcgacgagtt
     1861 cacgggacgt gttctggagg gcaggcgcta ctccgaggga ctccaccagg ccatagaggc
     1921 caaagaaggc gtggagatca aagaggagaa ccagaccgtc gccactatca cgatccagaa
     1981 cttcttcagg atgtacgaca agctctcagg catgacgggt acggccgcga ccgaggccga
     2041 cgagttcatg cacacataca agatggaagt cgtctccatc cccacgcaca aggagatgat
     2101 ccgggacgac aaggacgacc tggtatataa gagcgagaag tccaagcaca gggccgttgt
     2161 agaggatatc gtcgagcgcc acaagaaagg ccagcccgtg ctcgtcggca cggtctccgt
     2221 cgacgtctcg gagcgcctcg ccgggctgct caagcggagg ggcatcaggc acaacgtact
     2281 caacgccaag cagcacgaga gggaggcgga gatcatcgcc gaggccggcg agcggggcgc
     2341 cgtgaccata gcgaccaaca tggccggacg cggtaccgac atcaagcttg gcgagggggt
     2401 gtccgagctc gggggactct acgttctcgg cacagagcgc cacgaagcac gccgcataga
     2461 caaccagctc aggggtcgtt ccggcaggca gggggacccc ggagagtcgc ggttctatct
     2521 gtctttcgag gacgagctgt tgaggctctt cggcgggcag aagatgcagg ggatcatcga
     2581 ccgcatcggg ctggaggagg acgtgccgat cgaggccggt atgatctcca actcggtgag
     2641 gcgcgcccag gagcaggtcg agagccgcaa cttccagata aggaagcgca ttctcgaata
     2701 cgacgacgtc ctcaacaagc agcgagaggt gatatacacc ataaggcgcg acatcctgat
     2761 gggcgaggac gtggacacga tgtcctacgt cgaggacgtg ctcaccgagg ccgtctcgga
     2821 gcacgtctcc gcggacgtct atcccgagga ctgggacctt gacgcgctca ccgcagacgt
     2881 gaaccgcttc tatccgagca gggtcgatct caaatctctg gacgtggaga ggctgaccgc
     2941 caccgaggta ctcgcgatgg tcctcgaaga cgcccgcgag cgcctggatg agcgcaaggc
     3001 ggaatgggac gaacgtaccg ccgagctgga aaagcgcggg ctcgctcgcg gcgacggggt
     3061 ggctactttc gaggaggccg agcgcaggac gctgctatcg gtcgtagact cccgctggag
     3121 ggagcatctg tatgagatgg actatctgcg tgagggcatc ggctggcgcg gtctctccca
     3181 acgcgacccg ctcgtcgagt acaagcgcga gggcttcgac atcttccagg agatggagcg
     3241 tggcctgaag gaagactacg taacctacat ctaccgcgtc gagaacatca agctgcgcga
     3301 agaggagatg cggcagctct cctacagcgg tggtgaggac ccgagccaga gcaccaggag
     3361 cccccgccag cgaagctcgt cggtcaaagt cggccgcaac gagccgtgtc cgtgcggcag
     3421 cggcaagaag tacaagaagt gtcacgggat gccgggcgcg ccgccgctcg ggtcagaggc
     3481 ccgggcaggt tagtgaccga gctcgatgac aggagatccg agctggaagg gcgcctcgct
     3541 gaactggagg cgttcttcgg cgtggaaggg ttgcgggcag aggccgaagg gctgggcgag
     3601 gagatgagcc gtcccggttt ctgggacgac cccgaggagg ccaagacggt ctccgcccgc
     3661 ttctcacgcg tgcagggcag gatcgagctc ctcgacggcc tgcgagagcg cctctcggac
     3721 gcgggggagc tcctggagct ggccgaggaa gatggggagt tgctccgcga agtcggagaa
     3781 gaactacggc gggtcgcgcg ggtgatcgaa gagcaggagg ttgccaggct cttcgccggc
     3841 gagtacgacg atggcgacgc catcctaacc ataaactcgg gcgccggcgg cgtcgactcg
     3901 caggactggg ccgagatgct ggccaggatg tacaggcgct gggtcgagcg gcggggcttc
     3961 gggctcgagg tcatcgagta cacagaaggc gaggaggccg gcattaagag cgccaccttc
     4021 accgtctcgg gggagtacgc cttcgggctc ctctcggccg agcgcggggt gcatcgcctc
     4081 gtgaggatca gccctttcga cgcgggcgcc aggaggcaca ccagcttcgc ctccgtcgcc
     4141 gtcgcgcctg ttgtgggcga ggccgtcgag gtccagatcg aggagaaaga cctcaaggtg
     4201 gatacctaca gggcgtcggg tgccggcggt cagcacgtca acaagaccga ctccgcggtc
     4261 aggatcacac acctgccgac gggcatagtg gcgcagtgcc agaacgagcg gagccagcac
     4321 cagaaccgcg aggtcgccat gcgggtcttg agggctcggc tcttcgagct cgagcgcgag
     4381 aagcgggagc gggagatcgc agcgcagagc ggagagaagg ctgatataga gtgggggtcc
     4441 cagatccggt cctacgtcct tcacccttat aagctggtga aggaccaccg caccggcgag
     4501 gagacggcga acgtggacag ggtgctcgac ggcgacatag acgctttcat ctacgcctac
     4561 ctgaagaacc ggaccgcagc ctagcgccat gcggggcgtc ctcgctgccg tgctcgtgcc
     4621 cgtcgtgctc gtctgcctga tcgcgcctta cactttcctg ccttccctgc tcgagtacgc
     4681 ggtcgcccgc gac
//
LOCUS       DATAIR010000220         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_106059, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000220 DATAIR010000000
VERSION     DATAIR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106059"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VHM69_05270"
     CDS             <1..893
                     /locus_tag="VHM69_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2739836.1"
                     /translation="RENPVAWMQAHPDAIAHVMEYVRENGSVRSAEFRRADGRAGGWW
                     DWKPEKKALEHLFAAGELMVSRRENFHRVYDLRERVLSKALPLWEDAFAPTEQEVRRT
                     LALKAVRSLGVAVARWVPDYFRTPKKGVAALLEELADEGSLLRATIGDEPAYVHPDNA
                     RLAEAIVYGGVTSSVTTLLSPFDPVVWDRARALELFGFEYKIEVYTPAAQRRYGYYSL
                     PILHEGSLVGRLDAKAHRKDGLFEVKAIHLEPGVPVSDGLVSGIAGALRDCAGWHGTP
                     EVVVRGSDPPELAGPLRARI"
     gene            complement(932..2137)
                     /locus_tag="VHM69_05275"
     CDS             complement(932..2137)
                     /locus_tag="VHM69_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2739837.1"
                     /translation="MSGSAKKAIIVGGGIAGPVTAMALQRAGIEPVVYEAYASGAGDA
                     GAFLTVAVNGLDALRVLDAHRPVLATGFPTRNIEFFSGSGKRLGEVPIGGTLPDGTVT
                     HTIKRSDLYNALHEETIRRGIRIEHGKRLVDARTTPDGGVIAFFEDGTHAKGDLLIGA
                     DGIHSRTRAIIDPASPKPRYSGLGNIGGYSRDAPVTARTGQYEMVFGKRAFFGYAVSP
                     SGEIWWFANPPSERELTRAELAAATTEQWKERLIGLFSGDATPAVDIIRSTTGELTGT
                     NQYDMPSVPTWHRGPMIIVGDAAHATSPSSGQGASMAIEDAVVLARCLRDLPDVTQAF
                     AAYEQLRRQRVERVVAYGAKTNNSKAAGPVARVLRDLMLPIFLKRAAGDESFAWVYDY
                     HIDWQQKVA"
     gene            complement(2134..>2561)
                     /locus_tag="VHM69_05280"
     CDS             complement(2134..>2561)
                     /locus_tag="VHM69_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX2739838.1"
                     /translation="LDDWGRGDGDAYGSKFTEDADYVAFDGTHTKGRSDIAASHQQLF
                     DKYLKGSRLTGRIQSVRFLSPDTALVHAVGGTVMRGKTRPSPERDSIQTLVAVRRDGH
                     WRFAAFHNSRVRPISGGVAAFLVWAITDRLWKVFGRRSA"
BASE COUNT          416 a          823 c          845 g          477 t
ORIGIN      
        1 acagggaaaa tcccgtggcc tggatgcagg cccaccccga tgcgatagcg cacgtgatgg
       61 agtatgttcg cgagaacggg tccgtgcgct cggccgagtt ccggcgcgcc gacgggcgtg
      121 ccggcggctg gtgggactgg aaacccgaga agaaggcgct ggagcacctg ttcgccgcgg
      181 gagagctcat ggtttcgcgc cgcgagaact tccaccgcgt ctacgacctg cgcgaacgcg
      241 tgctgtcgaa agcgttacca ctctgggaag atgccttcgc gccgaccgaa caggaggtgc
      301 ggaggacgct cgcgctcaag gccgtgcgct ctctcggcgt cgccgttgcc cgctgggtcc
      361 ctgactactt ccgtacgccc aagaagggcg tagccgcgct gctggaggag ttggctgacg
      421 agggctctct acttcgggcc acgatcgggg acgagcccgc atacgtgcat ccggacaatg
      481 cgaggctagc cgaagcgatc gtctacggtg gggtgacatc ttccgtcacc actttgcttt
      541 cccccttcga tccggtggtt tgggatcggg cgcgagcact ggaactcttc ggcttcgagt
      601 acaagatcga ggtctacacc ccggctgccc aacgccggta cggatactac tcgctcccca
      661 tactgcacga aggctcgctc gttggcaggc tcgacgcgaa ggcccatcgc aaagatggcc
      721 tgttcgaggt aaaggcgata cacctcgagc ccggcgtacc ggtctccgac ggtctcgtct
      781 ccggcatcgc aggggcgctg cgcgattgcg cgggctggca cggcacccct gaggtagtgg
      841 tgcgaggctc cgatccgccg gagctcgccg ggcctctgcg ggccaggatc tagcgtacac
      901 cggagcgccg ggtccacgtg tcactccaca gtcacgccac cttctgttgc cagtcgatgt
      961 ggtagtcata gacccaagcg aaggactcgt cgccggcggc acgcttgagg aagatgggta
     1021 gcatcaggtc gcgcagcacc cttgcgacgg gtccggcggc tttactgttg ttcgttttcg
     1081 ctccgtaagc caccacgcgt tccaccctct ggcggcggag ctgctcgtag gcggcgaacg
     1141 cctgcgtgac atcggggaga tcccgcaggc acctggcgag gacgacggcg tcttcgatgg
     1201 ccatggaggc gccctgtcct gagcttggcg aggtggcgtg tgcggcgtcg ccgacgatga
     1261 tcatcggtcc cctgtgccac gtcggcaccg aaggcatgtc gtactggttg gtaccggtaa
     1321 gctctcctgt ggtggaacgg atgatatcga cggccggtgt cgcgtctccc gagaacagcc
     1381 cgatcaggcg ctctttccac tgctcggtcg tggcggccgc cagctcggcc ctggtcagtt
     1441 ctctctcgct gggtgggttg gcgaaccacc agatctcacc cgatggactg accgcgtagc
     1501 cgaagaacgc ccgcttgccg aagaccatct cgtactgccc ggtccttgcc gtgaccggag
     1561 cgtcgcgaga gtacccacct atgttaccca ggccagagta gcggggtttc ggtgaggcgg
     1621 gatctatgat ggcgcgggtg cgggagtgga tcccgtccgc gccgatgagc agatcgcctt
     1681 tggcatgtgt gccgtcctcg aagaatgcga tgacgccgcc atcgggcgtg gtcctggcgt
     1741 cgacgagccg cttgccgtgc tcgatacgaa tgccgcgccg gatcgtctct tcgtgcaacg
     1801 cgttgtatag atcagagcgc ttgatcgtgt gggtaacggt gccgtcaggc aacgtccccc
     1861 caattggaac ctcgcccaga cgcttgccgc tgccgctgaa gaactcgatg ttcctggttg
     1921 ggaagccggt cgccagcacc ggcctgtggg catcgagcac gcgcagggca tccaggccgt
     1981 tgacggcgac cgtgagaaac gcgccggcgt cgccagcgcc actggcgtac gcttcgtaga
     2041 cgaccggctc gatccctgct cgttgcaacg ccatcgcggt aaccggaccg gcaatgccgc
     2101 cgccgacgat aatggccttc tttgccgagc cgctcatgcg cttctccttc cgaagacctt
     2161 ccagagccgg tcggtgatcg cccagacgag gaacgccgcg accccgccgc ttatgggcct
     2221 gacgcggctg ttgtggaagg ctgcgaagcg ccagtgaccg tctcggcgca cggcgacgag
     2281 cgtctggatc gaatcgcgct ccggagaggg ccgcgtcttt ccgcgcatca cagtgccccc
     2341 gaccgcgtgt accagggcgg tgtcgggact caggaacctc acgctctgga tccggccagt
     2401 gagtcgtgag cctttgagat atttgtcgaa gagttgctgg tgcgaggcgg cgatgtcact
     2461 ccgccctttg gtgtgggttc cgtcgaacgc cacgtagtcc gcgtcctccg tgaacttcga
     2521 tccgtaggca tcgccgtcac ccctgcccca atcgtcgagc a
//
LOCUS       DATAIR010000221         4746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_13992, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000221 DATAIR010000000
VERSION     DATAIR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13992"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VHM69_05285"
     CDS             <1..229
                     /locus_tag="VHM69_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2739839.1"
                     /translation="SGEIGIHALRGGAVVGEHRLIFYSGGEEVEVVHRALSRRTFADG
                     AMRAARFVVRSQPGLYSIADVLAGGVQDLRD"
     gene            complement(246..1022)
                     /locus_tag="VHM69_05290"
     CDS             complement(246..1022)
                     /locus_tag="VHM69_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX2739840.1"
                     /translation="MMDEERGRSGHVRRRRTEDERLLSVPEREARRESTSWRVLRIMG
                     EFVEGFDQLAELGPAVTLFGSARISPGDPMYAAAVEVGRLLGEAGFTIITGGGPGAME
                     AGNMGAREAGAPSVGLNIELPFERHVNPYVDVEIDFRYFFVRKVMLVKYSRAFVIFPG
                     GFGTMDELFEALTLIQTGKIRDFPVVLFGSDYWRGLIEWLRANMVADGKATEADLGLI
                     ALTDSPEAVLEIIMSSAREQRERVDYEEEAREATRKALGG"
     gene            1093..2943
                     /locus_tag="VHM69_05295"
     CDS             1093..2943
                     /locus_tag="VHM69_05295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX2739841.1"
                     /translation="MKDTTSTVPLIPREVLFGNPEKARPLLSPDGERLAYLAPRDGVL
                     NVWVGPVGGDDFEPVTEDTRRGIRVYFWAEDNRHIVYLQDEGGDENWRVHAVDASTKE
                     DRDLTPFENVQAQIVDTNRRFPDTLLVALNRENPELHDVYRLTLSTGELDLAAKNPGN
                     VVGWVVDAQFRVRVAMAATPEGGFDLLLRDAEDAGWRKFLGWDKEDALSSSPVGFTGD
                     GEKMYLLDSRGANASRLVLLDLESGTSETLVEDPRYDVGRVMMHPETHEAQAAAVERA
                     RTDWVVLDDDIREDFEIIEGMNRGDFGVASRDRADENWLVAFTADDGGASYYAYDRKS
                     KQGEHFFDARPDLSEYHLARMEPVSFTSRDGLRIEGYLTLPPGSSTGPMPMVLNVHGG
                     PWVRDGWGYDPEAQWFANRGYASLQVNYRGSTGYGKKFLNAGNKEWGGRMHDDLVDAV
                     EWAVKQGVADPERVAIYGGSYGGYAALVGATFTPDLFRCAVDIVGPSSLMTLITSIPP
                     YWKPLVSTFHERVGNPETEEDFLKSRSPLFFVDRIRIPMLIAQGANDPRVKQAESEQI
                     VAPMKEKGIDYEYLLFEDEGHGFARPENRLKFYAAAERFLAKHLGGRAEE"
     gene            complement(2968..3132)
                     /locus_tag="VHM69_05300"
     CDS             complement(2968..3132)
                     /locus_tag="VHM69_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739842.1"
                     /translation="MEERGEVCLAEEVEQLRQLGLSDQEVALRLGVDAAWVETVISPE
                     DETAQEREAD"
     gene            complement(3199..3429)
                     /locus_tag="VHM69_05305"
     CDS             complement(3199..3429)
                     /locus_tag="VHM69_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX2739843.1"
                     /translation="MAAAITVALYTGGGAGAESQSIHYTVAPGDTLWGIAIEHTPPWE
                     DPRPKVEAIREANDLSGYEIFPGMSLELPSSV"
     gene            4139..4525
                     /locus_tag="VHM69_05310"
     CDS             4139..4525
                     /locus_tag="VHM69_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2739844.1"
                     /translation="MGGEGFTARVITNDIPGCPKGGLLLGTDKRDFLNGVDGDDVIRG
                     LGGKDGLMGGDGNDVIYGGPGGDSLLGGRGEDVLYGGDGNDFLWAKDGDRDKIYGGKG
                     KDMCTFDKLDHVDSSCEKKSRRMPQV"
BASE COUNT          927 a         1507 c         1473 g          839 t
ORIGIN      
        1 gtcaggtgag atcggcatcc acgccctgcg aggcggcgcg gtcgtcgggg agcaccgtct
       61 catcttctac tcgggtggcg aggaggtcga ggtcgtgcac cgcgccctct cccgccgcac
      121 cttcgccgac ggcgcaatga gagccgcccg gttcgtcgtg agatctcagc ctggcctgta
      181 ctccatagcc gatgtgctgg ccgggggcgt gcaggacctt cgagattagc gccggcccgg
      241 ttgggttatc ccccgagcgc ctttctggta gcctcgcggg cctcttcctc gtagtcgacc
      301 ctctcccgct gctcgcgcgc cgaggacatg atgatctcga gcaccgcctc gggtgagtcg
      361 gtgagggcta tcaggccaag gtcggcctcg gtggctttcc cgtctgcgac catgttggca
      421 cgcagccatt cgatcagacc tcgccagtaa tccgacccga acaggacgac ggggaagtcc
      481 cggatcttgc ccgtctgtat gagggtgagc gcctcgaaga gctcgtccat ggtcccgaag
      541 ccgccgggga agatgacgaa tgcccgcgag tacttcacga gcatgacctt gcggacgaag
      601 aagtacctga agtctatctc cacgtccaca tagggattta cgtgacgttc gaaaggcagc
      661 tctatattca gcccgacaga aggcgcaccc gcctcccttg cccccatgtt gcctgcctcc
      721 atcgctcccg gtcctccgcc agtgatgatg gtgaaacccg cttcgccgag gagccttccc
      781 acctcgacgg ccgccgcgta catcgggtcg ccggggctta tgcgcgcaga cccgaagagc
      841 gtcacggcgg gaccgagttc cgccagctga tcgaagccct cgacgaactc gcccatgatc
      901 ctcaagaccc gccacgaggt cgactccctg cgagcctccc gctcaggcac ggacagcagc
      961 cgctcgtcct ccgtgcgacg ccgacgtacg tgccccgacc tcccgcgttc ctcatccatc
     1021 attccaataa tctaccgcac atgtctcgcg gcgcgctaaa cttcgtggcg ctgactagat
     1081 agagaggaac acatgaaaga caccacttcc acagtacccc tgatcccacg cgaagtcctt
     1141 ttcggcaatc cggagaaggc aaggccgctc ctttcccccg acggggaacg cctcgcctac
     1201 ctcgccccga gagacggcgt cctgaacgtc tgggtcgggc ccgttggcgg cgacgacttc
     1261 gagcccgtga ccgaagacac gaggcgcggc atcagggtgt atttctgggc cgaggacaac
     1321 aggcacatcg tctacctcca ggacgagggc ggcgacgaga actggcgcgt gcacgccgtg
     1381 gatgcgtcaa cgaaagagga ccgcgacctc acccccttcg aaaacgtgca ggctcagatc
     1441 gtggacacca accgccgctt cccggatacg ctgcttgtcg cgctgaacag ggagaaccct
     1501 gagctccacg acgtctaccg cctgaccctc tcgaccggag agctggacct cgcggccaag
     1561 aaccccggca acgtcgtcgg ctgggtcgtc gacgcgcagt tcagggtgcg cgtcgcgatg
     1621 gcagccaccc cggaaggcgg cttcgacctg ctcctgcgcg acgcggaaga cgcgggctgg
     1681 cgcaagttcc tcggctggga caaggaggat gcgctctcca gttcccccgt cggctttacc
     1741 ggggacggcg agaagatgta ccttctcgac tcgcgcgggg ccaacgcctc acgcctcgtc
     1801 ctgctagatc tcgaaagcgg tacttcagag acgctcgtcg aggacccccg ctacgacgtt
     1861 ggccgggtca tgatgcatcc ggagacccac gaggcccagg ccgccgccgt cgagcgcgcg
     1921 aggaccgatt gggtcgtgct cgacgacgac atcagggaag atttcgagat catagagggc
     1981 atgaaccgcg gggacttcgg cgtggcgagc cgcgacaggg ctgacgagaa ctggctcgtc
     2041 gccttcaccg cggacgacgg cggcgcctcg tactacgcct acgaccgaaa gagtaagcag
     2101 ggcgaacact tcttcgatgc ccgtccagac ttatctgaat accacctggc caggatggag
     2161 cccgtctcct tcacctcccg cgacggcctg aggatagagg ggtacctgac gctgccgcca
     2221 ggaagttcca ccggtcccat gccgatggtc ttgaacgtgc acggaggtcc gtgggtgcgg
     2281 gacgggtggg gctacgaccc cgaagcgcag tggttcgcca atcgcgggta tgcctccctg
     2341 caggtcaact acaggggctc gacagggtac ggcaagaaat tcctcaacgc ggggaacaag
     2401 gagtggggcg gcaggatgca cgacgacctc gtggacgcgg tcgagtgggc cgtaaagcag
     2461 ggcgtggccg accccgagcg tgtcgccatc tacggcggct cctacggagg atacgccgcg
     2521 ctcgtcgggg ccacgttcac gcctgacctc ttcagatgcg ccgtagacat cgtgggtccg
     2581 tcgagcctga tgaccctgat aaccagcatc ccgccgtact ggaagccgct ggtctccacc
     2641 ttccacgaac gggtcggcaa ccccgaaacg gaggaggact tcctcaagag ccgctcgccg
     2701 ctctttttcg tggaccgcat caggataccg atgctcatag cccagggtgc caacgacccg
     2761 cgcgtgaagc aggccgagtc ggagcagatc gtcgccccca tgaaagagaa aggcatcgac
     2821 tacgagtacc ttctcttcga ggacgaaggc cacggcttcg cccgccccga gaaccgcctg
     2881 aagttctacg cagccgcaga acgcttcctc gcgaagcacc tcggcggacg agcagaagaa
     2941 tagaggatgt cgcgagcggg gagtcgctca gtccgcttcc cgctcctggg cggtttcatc
     3001 ctccggtgag atcaccgtct caacccaggc cgcgtccaca cccagcctga gcgcaacctc
     3061 ctggtcggag aggccgagct gcctcagttg ctcgacctcc tcggccagac agacttcgcc
     3121 gcgttcctcc acgttctcac ctcagtcact cagcgatcct acgattggtg gagggtacca
     3181 cggacgtggt acggcggttc aaacggacga aggaagttcc aggctcatgc cggggaagat
     3241 ctcatatccc gagaggtcgt tggcctcacg gatagcctcg actttcggcc tcgggtcctc
     3301 ccagggcggc gtgtgctcga tggctattcc ccagagcgtg tctccgggcg cgacggtgta
     3361 atggatggac tgagactccg cccctgcccc gccgcccgtg tagagcgcga cggtgatagc
     3421 ggcggccacg aacaaggccc ctagcctgcg gcgacgatat attgcgcggt cctttctcac
     3481 cggacaaata tacgggacga acgcctctct cccaacggtc ttttcaagct ctccgtcagg
     3541 tagaattccc atatgcactt caccaagatg caacttcgtg ggtgctaacg gagtcacgtt
     3601 ccacgtccgc cttgaacccg acggcgtggc gagagtgcga ctcatcgggt agccgccaag
     3661 tgccggagaa gaaaacgagt cgcgttcacc ccagtcgtga ggtatccagc agggtgaatc
     3721 cttattcact ctgcccacat gttgtgttgg ggctacagtt ctcacgggac ttgtaggaac
     3781 tcttctccgc taccgacact gagttataca ggatgctgga ttcggacttc cgagaattag
     3841 ccttccactc cggtgaataa gcctccatag agatgcgcca gggtgcgttg tgtggcatgc
     3901 ttggaggtgc tagtagcaag gaacacatgt aagggaaggc gggtgcgtcg tagttcgccg
     3961 agggcacctc atagccttgg tgagagcctc cctgatcggg tggaccgttc ttcttctagc
     4021 ggttggctgt gcagggacga gctcggaaac ctccaagaag gagcagggac gctcacccct
     4081 ggcaacggcc tcagaagaag cccgatgcca ggggacgagg accaccaagg acaacccgat
     4141 gggcggggaa ggctttaccg cgcgcgttat cacgaacgac atacccggct gtcccaaggg
     4201 cggcctgctc ttgggcaccg acaaacgaga cttcctaaac ggggtggacg gcgatgacgt
     4261 gatacgtggc cttggcggta aagacggcct catgggggga gatggcaacg acgtcatcta
     4321 cggtgggccg ggcggcgaca gccttctagg aggccggggc gaggatgtcc tctatggcgg
     4381 ggatggcaat gattttcttt gggccaaaga tggggatcgg gacaagatct atggcggcaa
     4441 aggcaaggac atgtgtactt ttgacaagct tgaccatgta gacagcagtt gcgaaaagaa
     4501 gtcgcgccga atgccgcagg tataggcccg gggctatgcg gacttctgag aactccgtca
     4561 aggcaaagtt cgcagagtca caattctgcg tcgttgggtg aatgccgcga acgtagccct
     4621 cgcgaacctg tcgcgatggt gcacgggcgt gcacctgatc aactatcatg gctgcatggc
     4681 cgttgacctg tacgcgggca tcccggttaa tgactacccg gcggcgctgg cctggtacga
     4741 aaagct
//
LOCUS       DATAIR010000222         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_14008, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000222 DATAIR010000000
VERSION     DATAIR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14008"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1729
                     /locus_tag="VHM69_05315"
     CDS             70..>1729
                     /locus_tag="VHM69_05315"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739845.1"
                     /translation="MRGHPLQDTDKYRLLLELSACLAGRIKEGGTHSGGMVFGTAEHH
                     LSELVPLEPSGTEGLLRCQYDKYDLERVGLPKLDLLGLRMHTALHRAGVLASARLGHR
                     VDPYDPPPDDKKTYALIRTGLNAGMFQLESPGQMHLSRRLGPRRLADLVAQISLFRPG
                     PVRGDLVTPYVMRKNGQEPYSAILPELEEVLRRTYGVLVFQEQVLEVANAVAGFTLAE
                     GDLLRRAMTKDRGPGAMQDIRREFLRRARERGVPAGKADEVFSWMEGFSVYGFSAAHG
                     ASFAELAYASAYMRCHYPAEFFAAVLDSQPMGFYSPRVLLNEARRTGLRVLPPDIHLS
                     DEGFTVEEGSALRVGLRYCKGLSGRAISSIIAERRKRPFVSVADLYGRTAVEKDSIEN
                     LIRGGFLDSLSERSRLLGESAALPKKRRGRQEEIPVEHPASWWVSREMRSVEHLPLTD
                     TAKEGMEWEVLGLNVHRHPLVPYRGALEELGVVPSEQIRSLPHGTRASAAGMIECLQC
                     PPTKSGHSVYFFVVEDEWGMLQATSFRPVYERYGDLLHHEGAFLV"
BASE COUNT          311 a          528 c          593 g          297 t
ORIGIN      
        1 cgcttttcgg accggaacag ggtctacgca ggcctctccg gttgggaaga atcactggcg
       61 gagcccgcca tgcgcggaca tccacttcag gacacggata agtatcggct ccttctggag
      121 ctctcggcgt gtctcgccgg gcgcatcaag gagggaggaa cccatagcgg gggtatggtc
      181 ttcgggacgg cggagcatca tctctccgaa ctcgtgccgc tggagccttc ggggacggag
      241 gggctattgc gctgccagta cgacaagtac gacctggaac gtgtgggcct tccgaagctg
      301 gacctgctgg gactaaggat gcacacggcg ctgcacagag ccggtgtcct cgcatcagcg
      361 aggctcggac acagggttga tccatacgac ccgccgccgg atgacaagaa gacgtacgcc
      421 ctcatcagga ccggtctgaa cgcggggatg ttccagttgg agtcgccggg gcagatgcac
      481 ctgagccggc ggctggggcc caggagactc gctgaccttg tggcccagat ctccctcttc
      541 aggccgggcc ccgtgagggg agacctcgtc accccgtacg tcatgaggaa gaacggacag
      601 gaaccatact cggcgatcct gcccgagctc gaagaggtgc ttcgccgcac ctacggcgtc
      661 ctcgtcttcc aggagcaggt tctcgaggtt gcgaacgccg tggccggctt caccctggct
      721 gagggagacc tcctgcggcg cgccatgacc aaggaccgcg gccctggcgc gatgcaggac
      781 atccgcaggg agttcctgcg gcgggcgcgg gagagaggtg tccccgccgg gaaagcggac
      841 gaggtcttct cgtggatgga ggggttcagc gtctacggtt tctccgccgc gcacggcgcc
      901 agcttcgctg agctcgccta cgcgagcgcg tacatgaggt gccactatcc ggctgagttc
      961 ttcgctgccg tcctcgactc ccagcctatg ggtttctaca gcccgcgggt actactgaac
     1021 gaggcgcgca ggaccgggct ccgcgtgctc ccgcctgaca tccatctctc ggacgaaggt
     1081 ttcaccgtgg aagaaggctc ggcgctccgc gtgggactcc gctactgcaa agggctctcg
     1141 gggagggcta tctcctccat aatcgccgag cgtcgcaaga ggccgttcgt ttccgtcgcc
     1201 gacctgtacg ggaggacggc tgttgagaag gactcgatcg aaaacctcat caggggaggc
     1261 ttcctcgatt ctctgtcgga gcggtcgcgg ctgctcggag agagtgcggc gctgccgaag
     1321 aagcggcgcg gaaggcagga ggagatcccg gtagagcatc ctgcgagctg gtgggtatcg
     1381 agggagatga ggagcgtcga gcatctcccg ctcacggaca cggcgaaaga agggatggag
     1441 tgggaggtac tcggcctcaa cgtccatcgt catcctctcg tgccataccg gggggcactc
     1501 gaagagctcg gggtggttcc gagcgagcag atcaggtcgc ttccgcacgg cacccgcgcc
     1561 agcgccgccg gcatgatcga atgtctccag tgcccgccga cgaagagcgg gcacagcgtg
     1621 tacttcttcg tcgtcgagga cgagtgggga atgctgcagg cgacgagctt ccgccccgtc
     1681 tacgagaggt acggcgatct gctgcaccac gaaggagcgt tcctcgtag
//
LOCUS       DATAIR010000223         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_190717, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000223 DATAIR010000000
VERSION     DATAIR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190717"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHM69_05320"
     CDS             <1..244
                     /locus_tag="VHM69_05320"
                     /inference="COORDINATES: protein motif:HMM:NF037143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739846.1"
                     /translation="EVLFRGATHDIVKDGGGYDVVFHLPLVEKRNVDLSKKGAELFIK
                     VGNYRRNILLPDALARLPAAGASFDGDLLKVRLNYE"
     gene            237..440
                     /locus_tag="VHM69_05325"
     CDS             237..440
                     /locus_tag="VHM69_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739847.1"
                     /translation="MSEERERRGSLLGVVAYVLVPGERRVRAAGHFRKAGFEALRGVG
                     ALMRPEGSGESKETTRRERIEIE"
     gene            500..820
                     /locus_tag="VHM69_05330"
     CDS             500..820
                     /locus_tag="VHM69_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739848.1"
                     /translation="MTTASFVKWPTPFQVKRVSNSVLGKELSFFFVSGVLRPGQYEEL
                     KRVIPVLAVHEVEDKLIVSTLITQEKCEELYREFEPLSRRWRNQYYVLANEIVRERRK
                     ALED"
     gene            872..1072
                     /locus_tag="VHM69_05335"
     CDS             872..1072
                     /locus_tag="VHM69_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739849.1"
                     /translation="MKDSSEKQEGAATEPAVRARVLRKVADFARQETTLALPAAKRAA
                     VHGAQAYFKKFSEEYAKERKKL"
     gene            1169..2065
                     /gene="pdxS"
                     /locus_tag="VHM69_05340"
     CDS             1169..2065
                     /gene="pdxS"
                     /locus_tag="VHM69_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2739850.1"
                     /translation="MAENGQLTGTFRVKSGMAQMLKGGVIMDVVDAEQAKIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEKIVEIQEAVTIPVMAKARIGHFVEAQILEALEV
                     DYIDESEVLTPADEKHHIDKAAFEVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRRLGALEHEELMTEAKDLGAPYELVRWVAENGRLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSGDPALRARAIVKATTHFKDAKVVAEASRGLG
                     EAMVGREMGDLAEGERLATRGW"
     gene            2055..2669
                     /gene="pdxT"
                     /locus_tag="VHM69_05345"
     CDS             2055..2669
                     /gene="pdxT"
                     /locus_tag="VHM69_05345"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2739851.1"
                     /translation="MAGNGETNGAARKGPKRVGVLALQGDFREHAEILRKLGVEPVEV
                     RAVEDLDGLAGIIVPGGESTTIGKMMVSSGLLDGIRSYFYKGGPVWGTCAGMVLAASA
                     TTGQRQPLLGLMNALVERNGFGRQVHSFEKDLDVEGFDEPFTGIFIRAPYFEDVGPGV
                     EVLSKIGDRVVAARGENILVTAFHPELTDDMRFHEYFLREVCSI"
     gene            2666..3430
                     /locus_tag="VHM69_05350"
     CDS             2666..3430
                     /locus_tag="VHM69_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2739852.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSRAITVAAREGGGDVE
                     MNPALGLAVHKAKAGNMPNDNIQRAIDKGTGAGADADAFERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYIFSKNGGKLGTSGSVAYLFERKGVILVPKDSTDEDELMEVALEA
                     GADDVEEHQNDYRVVTTPEGFTAVRDALAEADIAFENAEITMEPQNSVDLDASTAKQT
                     LRLIDALEENDDVQEVYANFDISDEVMAVTIDEEVT"
     gene            3630..3929
                     /locus_tag="VHM69_05355"
     CDS             3630..3929
                     /locus_tag="VHM69_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2739853.1"
                     /translation="MTYLRCTMGSWDIDLNSEEGREAFRLIDDDGLRVFRNQPGFIRY
                     RLMVADPTTTIAVAEWESEELGKPGAQRYREWLTSSGIAEKLSLETFDGPVVVAS"
     gene            3954..4886
                     /locus_tag="VHM69_05360"
     CDS             3954..4886
                     /locus_tag="VHM69_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2739854.1"
                     /translation="MDYGAHLPLIALSGRRWSLRSLLEYTELAERLGFSALSANDHLL
                     FPRPWLDGPTAMAAVLARTGEMALATTVALPVVRGPVALAKSLAALDLLSGGRLVVGV
                     GPGSSARDYDAAGIPFGERWKRLEEAVQAMRVLWNREGPAFEGEFYSTEGIVLEPYPA
                     QQPGPPIWIGSWGSEAGLRRTARLGDGWLASAYNTTPEAFANALGRLGELLSAAGKDP
                     DHFPNAVATMFFYVTEDRTIAGRIIREVLSPTLNRPEQEVSQRLLVGSPGECAEKLAA
                     YQGARAQRIYLWPVKDELRQLEIFMERVAPAVCT"
     gene            5075..>5573
                     /locus_tag="VHM69_05365"
     CDS             5075..>5573
                     /locus_tag="VHM69_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739855.1"
                     /translation="MLKTDTVVDASGIDTLNFSAMSSGIRASLHPGDGLFPANWVNDS
                     SFARLVTVSSNSGNVIEKVVGSASGDDFIQTGRAANILQPGPGRDGATLIDIGGCNSS
                     NQSSPPDCGAPPVPVSTDTYSGFSASGYGAVRISDLGGTADKLILPFATADAYFEASD
                     EDGNGS"
BASE COUNT         1173 a         1532 c         1860 g         1008 t
ORIGIN      
        1 ggaagttctg ttccggggcg ccacgcatga catagtgaag gacggcggcg gatacgatgt
       61 cgtcttccac ctcccgctgg tggagaaacg gaacgtggac ctctcgaaga agggcgcgga
      121 gttgttcata aaggtgggca attatcggcg caacatcctg ctccccgatg ctctcgcccg
      181 tctcccggcg gccggggcga gcttcgacgg agacctgctg aaggtgaggt tgaattatga
      241 gtgaggagag ggaacgcaga ggctcgctgc ttggcgtggt cgcctacgtc ctcgtaccgg
      301 gcgagcggcg cgtgcgtgca gccgggcact tccggaaggc ggggttcgag gcacttagag
      361 gcgtcggggc gctgatgcgc cccgagggat cgggcgaatc caaggaaacg acgcggcgcg
      421 agcggatcga gatcgagtag gattgtcgct gttggcctga caaaaataca acagtttggc
      481 aatgaaaaga ggagttgagt tgacgaccgc atcgtttgtt aagtggccta ctccattcca
      541 ggtaaagagg gtctctaact ctgttcttgg caaagagttg tcgttctttt ttgtgtcggg
      601 ggtattgaga ccgggacagt atgaggagct caaaagagtg atacccgtgc tagccgtgca
      661 tgaggtggag gacaaactga tcgtctctac gctaatcacc caggagaagt gcgaagaact
      721 ctaccgggag tttgaaccac tttcgagacg ttggagaaat cagtattacg tacttgcgaa
      781 cgagatcgtt cgggaacgta ggaaggcgct ggaagactaa cctgaaggca cctggcagct
      841 tacaatctga agacgggtcg cacgagatcg aatgaaggat agttccgaaa aacaggaggg
      901 cgcggcgacg gagccagccg taagagcccg agtgctgcgg aaggtggcgg atttcgcgcg
      961 ccaggagaca accctggcgc tccctgcggc gaagcgtgcg gctgtacacg gagcacaggc
     1021 ttacttcaag aagttttccg aagagtacgc gaaagagcgc aagaagctgt agaagattct
     1081 ggctttcgat ctgcgctggt ttggcgcgat ggggcgctgg tagtaagata tcccttacaa
     1141 attctagtag atacccattg gagattatat ggcggagaac ggacagttaa cagggacctt
     1201 cagggtcaag agcgggatgg cccagatgct caagggcggg gtcatcatgg acgtcgtcga
     1261 cgctgagcag gcgaagatag ccgaggaggc tggggccgtc gcggtcatgg cgctggagcg
     1321 cgtgccggcg gacataaggg ccgatggtgg cgtcgccagg atgagcgatc ccgagaagat
     1381 cgtggagatc caggaagccg tcaccatacc cgtgatggcg aaggcgcgca tcggacactt
     1441 cgtggaggcc cagatcctcg aggccctcga ggtcgactat atagacgagt ccgaggtgct
     1501 aacccccgcc gacgagaagc accacataga caaggccgcc ttcgaggtgc ctttcgtgtg
     1561 cggcgcgacg aacctcgggg aggcgctgcg ccgcatcggc gaaggggccg ctatgatccg
     1621 ctcgaagggt gaagcgggaa cgggcaacgt cgtcgaggcc gtgcgccaca tgcgggccat
     1681 agtcggcggc atccggcgcc tgggcgccct cgaacacgag gagctgatga cggaggcgaa
     1741 ggacctcggg gcgccatacg agctggtgcg gtgggtcgcc gagaatgggc ggcttcccgt
     1801 ggtgctgttc accgccggcg gcatagcgac tccggccgac gctgcgctca tgatgcagct
     1861 cggggcggac ggcgtgttcg tggggagcgg catcttcaag agtggggatc ccgcccttcg
     1921 cgcccgggcc atcgtgaagg cgaccaccca cttcaaggac gccaaggtcg tcgcggaggc
     1981 gagcaggggt ctcggcgagg cgatggtagg cagggagatg ggcgacctcg cggaaggaga
     2041 gcggctcgct acccgtggct ggtaacggcg agacaaacgg cgcggcacgc aagggcccaa
     2101 aacgggtcgg ggttcttgcc ctgcagggag acttcaggga gcacgcggag atactgagaa
     2161 agctaggcgt tgagcccgtc gaggtaaggg ccgtcgagga cctcgatggt cttgccggga
     2221 tcatcgtccc gggcggcgag tcgactacca tcgggaagat gatggtctca tccggcctcc
     2281 tcgacggtat caggagctac ttctacaagg gcggcccggt gtggggcacg tgcgccggga
     2341 tggtcctcgc ggcgtctgcg acgacggggc agcgccagcc cttgctcggt ctgatgaacg
     2401 cgctcgtcga gcgaaacggg ttcggacgtc aggtccattc cttcgagaag gacctcgacg
     2461 tcgaggggtt cgacgaaccg ttcaccggga tctttatccg tgcgccgtac ttcgaggacg
     2521 tcgggccagg ggttgaagtt ctttcaaaga taggcgacag ggtcgttgcg gcgaggggag
     2581 agaacatcct cgtaacagcg tttcaccccg agctcacgga cgacatgagg tttcacgaat
     2641 atttcttgcg ggaggtttgc tcaatatgag tggtcatagc aagtggtcga cgatcaagcg
     2701 caaaaaaggc gctgcagacg cgaagcgagg ggcgctgttc gggaagctgt cgagggcgat
     2761 cacggtcgca gcccgcgagg gcggcgggga cgtggagatg aatccggcgc tcggcctggc
     2821 ggtacacaag gcgaaggccg gcaacatgcc caacgacaac atccagcgcg ccatcgacaa
     2881 ggggaccggc gcgggcgccg acgccgacgc cttcgagcgg atcacctacg aaggatacgc
     2941 cccaggcggg gtcgccgtcc tcgtcgacgt gcttaccgac aaccgcaaca gggccgcgtc
     3001 ggacgtgcgc tatatcttct ccaagaacgg gggcaagctc ggtacgagcg gctcggtcgc
     3061 ctacctcttc gagcgcaagg gcgtcatcct cgtccccaag gactccacgg acgaggacga
     3121 gttgatggaa gtcgccctcg aagccggggc cgatgacgta gaggaacacc agaacgacta
     3181 cagggtcgtt accacgcctg agggcttcac ggctgtgcgc gacgccctcg ccgaggcgga
     3241 catcgcgttc gagaacgcgg agatcacaat ggagccccag aacagcgtcg acctcgacgc
     3301 ctcgaccgcg aagcagacgc tgcgcctgat cgacgccctc gaagagaacg acgatgtgca
     3361 ggaagtctac gcgaacttcg atatctcgga cgaggtcatg gcggttacaa tcgacgaaga
     3421 agtaacgtag gctaacaaga cgtgcgggga taggaatgga tcctcgcact cccgcaaaac
     3481 gtttcggaag gcaaatcctt ccgaaacgtc cttgaaaaac ggacgtattg atgtgagcct
     3541 tcgtcagaat acgcgatccg gaaaatgcgc ctacctcgta gtttcgggta tcgagaagac
     3601 aatttcatag cggcaatcag ggaggatcgg tgacgtattt gcgctgcacg atggggagtt
     3661 gggacatcga tctaaactct gaggaaggtc gggaggcgtt tcgtctcata gatgatgatg
     3721 ggctccgggt cttcaggaac cagccagggt ttatccgtta caggctgatg gtcgcagacc
     3781 ccaccacgac aatcgcggtc gccgagtggg agtccgagga gcttggtaag cccggcgcac
     3841 aacgctacag ggaatggtta acaagcagtg ggatagccga gaagctgtca ctggagacat
     3901 tcgacggtcc ggttgtggtc gcttcctgaa ttaccagggt gagaggaggc caggtggatt
     3961 acggagcaca cctaccgcta atagccctca gcgggcggcg ctggtctcta cgaagtctcc
     4021 ttgagtatac ggagttggcc gaacgcctgg gcttcagtgc tctctcggcc aacgaccacc
     4081 tgctctttcc ccgtccttgg ctcgatggac cgacggccat ggccgcggta cttgcccgca
     4141 cgggagagat ggctctcgca accacggtag cgctccccgt cgtgcgaggg ccggtggcgc
     4201 tggcgaagag cctggctgcc ctcgacctcc tctccggcgg tcgcctcgtc gtaggagtgg
     4261 ggccaggctc ttccgccaga gattacgacg cggccggcat ccccttcggg gagcgctgga
     4321 agcgtctgga ggaggcggtg caggcgatgc gcgtcctctg gaatagggag ggtcccgcgt
     4381 tcgagggtga gttctactcc acagaaggca tcgtgctgga gccttatccg gcacagcaac
     4441 cagggcctcc tatctggatc gggagctggg ggtcagaggc cggtctcagg agaacggccc
     4501 gccttggcga cggctggctg gcttctgcct acaacaccac cccggaagcg ttcgccaacg
     4561 cattgggacg cctcggagaa ctcctgagcg ccgcaggcaa ggaccccgat catttcccca
     4621 acgccgtagc gacgatgttt ttctatgtga ccgaggaccg caccattgcg ggaaggatca
     4681 ttcgggaggt cttgagcccg acgctcaacc ggcctgaaca agaggtgagc cagcgtctcc
     4741 tggttggctc ccctggagaa tgcgcggaga agcttgcagc ctaccaggga gcaagagcac
     4801 aacggatcta cttgtggcca gtgaaggacg agttgcgcca gctggaaatc tttatggagc
     4861 gagtggcccc ggcagtctgc acgtagtttc gactcactgt tcgagatagc gagtcgtttg
     4921 gccgaccttc tgaaaacgta gtgcaggcga actctggaaa atatgtcacc tggcgtatgt
     4981 acaaaccctc attcatctgg cacgttgaga gcgatggcat cactatgagg cggcacttgc
     5041 ctttctcccg cctccagact cccagggaaa gaaggtgctg aagacagaca cggtcgttga
     5101 tgcctctggc attgacaccc tcaacttctc tgcgatgtcc agcggaatcc gtgccagcct
     5161 gcatccaggt gacggtttat tcccggcaaa ctgggtgaac gatagttcgt ttgcacgact
     5221 ggtcactgtt tcttccaact cagggaatgt catcgagaag gttgtgggtt ccgcaagtgg
     5281 ggatgacttc atccagactg gaagggcagc caatatattg cagcccggtc ccggacggga
     5341 cggtgccacc ttgatcgaca ttggcggctg caacagcagc aaccagagtt cccctcctga
     5401 ctgcggcgcc ccgcctgtcc ccgtgagcac tgacacttac tcgggcttct cggccagtgg
     5461 ttacggagcg gtccgtattt cggaccttgg gggcacggca gacaaactca tccttccgtt
     5521 tgccactgcc gatgcctact tcgaggcttc cgacgaagat gggaacggta gtc
//
LOCUS       DATAIR010000224         6441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_14118, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000224 DATAIR010000000
VERSION     DATAIR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14118"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..988)
                     /locus_tag="VHM69_05370"
     CDS             complement(107..988)
                     /locus_tag="VHM69_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2739856.1"
                     /translation="MAQATVRTESEKSRRSPEQLSRLVLSYSALILLTLVFVTPLLWM
                     LSTSFKTNVEATQIPPNWIPEEFTLENYSTIFSTGESQTPVLRWFLNSMIAASAHTAL
                     VLVTASMASYALARMNFPGKSVLFATIITTLFIPTFIFLMPNYLIVDSLGWLDTLWAV
                     IVPGAAGAFGVFFLRQFFLSLPYELEEAALIDGASRIQIFLRIILPLSKAPLATLAVL
                     SFLNNWNDFLWPVYVLFSPRSLVLPPGLSKLQGAYDTDYAVIMAGGVLASIPVIILFI
                     IAQRYIIEGVSRSGLKG"
     gene            complement(992..1906)
                     /locus_tag="VHM69_05375"
     CDS             complement(992..1906)
                     /locus_tag="VHM69_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2739857.1"
                     /translation="MSKTLVGARKPTDPHPVTPYLFMAPYLVLLGVFILYPVFFGVWI
                     SLHNWDYLLPGKPWVGLENYTDLFTPGSTTSGPFWQSMEATGKFTLYSVPLLVVIPLL
                     VALMLNQSFRGRAFFRTVYFAPYVLSVAVIGILWRYLLDANVGLVNYYLGQLGLPDNI
                     PWTNALPWAWIALVGPTVWWTLGFNSVIYLAGLQDIDPQLYDAAKVDGANRWRRFLHV
                     TLPGLRPILLFVIVVTILASANMFGQAYLITQGGPGDDTRPAIMYLAEEGLQNFNMGS
                     AAAMSYVLAFCLAVISLANFALLRNRED"
     gene            complement(1955..3304)
                     /locus_tag="VHM69_05380"
     CDS             complement(1955..3304)
                     /locus_tag="VHM69_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2739858.1"
                     /translation="MDGTRARGRRLTRQDFLLLGAGAGAGLMLAGCGGGPENNPAVQQ
                     QGGGGGGKEYNGPKVDLAFWNGFTGGDGPLMRDLVEQFSSEHDNIDVTMNTVEWETYY
                     QKVPASVSTGEGPDVGIMHIDTVATNAARSVIIPLDDVANALKLKESDFVPIVWNAGI
                     YEGSRYGIPLDVHPFGDFYNKDVMEKGGLDPNKPPQTKDEFMAALEELKGKGVQGFWV
                     PTVPAGSTWIFETLLWQYGGELYNEDVTAATWNSDAGVDALTWLVDLIEQGYSPKNVG
                     DGAELIAFQNNNNAFYWSGIWNINPLKEAGVNYGAVPVPQIGSQKATWANSHNFVITN
                     KRGQDPNKITASKVFINWVSTHSAEWAKAGQVPARNSVRESPEFQKLEFQSNLAEQLP
                     YVRFPPSVPGIGDVQLNALDPAVNEALLLKKDPKTALDESASQAKQLLEENRQKYQA"
     gene            complement(3529..5442)
                     /locus_tag="VHM69_05385"
     CDS             complement(3529..5442)
                     /locus_tag="VHM69_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX2739859.1"
                     /translation="MLDSMPRPPVVVDTSRSRHARLRPLSLPDVRLSDGFWEPRRRIN
                     REKTLPSQFEHIEETGRLDNFRRASGKIDAPFRGLYFNDSDVYKWLEASSWSLAEGPD
                     PELTRMIDIAITEIEDAQQTDGYLNTYFTFERASERWTDFDLHEMYCAGHLFQAAVAH
                     FYATGSERLLGVAVRFADHICDVFGQDEQGKRVAVDGHEEIEMALVELFRATGNRRYL
                     EQARFFVDARGHGFLGRPYGLHEPSYSQDHEPLREQSETVGHAVRAMYLYCGAADVYA
                     EIGDGALLAALQNLWRNMTTRRMYVSGGLGSRYEGEAFGDDYELPNERAYAETCAAIG
                     SVMWNWRMLMLGGDARYADLIEHTLYNAVLLGVSLDGGHYFYQNPLADEGKHRRQPWF
                     GCACCPPNVARLLASLPGYFYGASEDAVWVHLYAEGVATVELDRNRVVRLRQRTGYPW
                     DGGVEVTVEGDGEFTMMLRIPSWCEEGASVQVNGEPLGEEVSPGTYLPIRRVWRPGDT
                     VSMNLPMLVRRVACHPYVAENEGRVALMRGPILYCAEQTDNPDVDLHMLVLEDEDPST
                     RFEPDLLGGVVVLEVGAQNVEPDDGWEGRLYRNAHRRERNIKPGATKLTAVPYFAWAN
                     REQGSMRVWLKTR"
     gene            complement(5451..>6441)
                     /locus_tag="VHM69_05390"
     CDS             complement(5451..>6441)
                     /locus_tag="VHM69_05390"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2739860.1"
                     /translation="VKYWGLGNEMYGEWQIGALTAEEYVRKAREFAKVMRWTDPNIEL
                     VGCGLSGLTDWDRVVIEGLAPFVDYHSIHIYTGSDDYWSNVLAPHQADRALRTTRAMI
                     ERARYEQRIDHPIHVAYDEWNVWFREREQTSGLEERYNLSDALAVATYLNVFARHCET
                     VKIANLAQMVNVIAPIFTNEEGLFLQTIYHPLRLCAEHMHDVVLDAHVASEKHDLTTE
                     ESSWPHRVADMGPFDLLDVSATCDAEGRSLTLVVVNRDPEQAMQTTIQLADSTFEGNA
                     TVYEVTGKDPAGINDFDHQTVGVSERSVRTEGRSFEHSFPACSVTVLVAGLAG"
BASE COUNT         1171 a         1978 c         2021 g         1271 t
ORIGIN      
        1 atgatgtagc ggccgtcgtc cttcaattcg cgctccttca cgaaagtagc ctctccctct
       61 tcggggcgcg atgcctcctc gcggggagcg gggtctctct tctcgctcat cccttcagcc
      121 cgctacgcga gacgccctcg atgatgtagc gctgggcgat tatgaacagg atgatgacgg
      181 ggatgctggc gagcacgccg ccggccatga tgactgcgta gtcggtgtcg taggcgccct
      241 ggagcttgga gaggcccggc ggcaggacga ggctcctcgg ggagaagagc acgtagaccg
      301 gccagagaaa gtcgttccag ttattcagga agctcaatac cgcaagcgtt gccagaggag
      361 ctttggagag tggcaggatg atgcgcagga agatctgtat gcggctggct ccgtctatga
      421 gggccgcctc ctccaactcg tagggcaacg aaaggaagaa ctggcgcagg aagaaaaccc
      481 cgaaagctcc ggcggccccg ggcacgatta cggcccacaa agtgtctagc caccccagcg
      541 aatcgactat caggtaattg ggcatgagga agatgaacgt ggggatgaac agcgtggtga
      601 tgatggtcgc gaacagaacg ctctttccgg ggaagttcat cctggccaga gcgtaggatg
      661 ccatggaggc cgtgacgagc accagcgccg tgtgcgcgga ggcggctatc atgctgttga
      721 ggaaccagcg gaggacgggc gtctgcgact cgcccgtcga gaatatggtg gagtagttct
      781 cgagcgtgaa ttcctcgggg atccagttcg ggggtatctg ggtggcttcg acgttagtct
      841 tgaaggaggt cgagagcatc cacagcagcg gggtcacgaa cacgagggtc aacaggatca
      901 gcgcggagta ggataggacc agccgggaga gctgctcggg actccgccgg ctcttctccg
      961 attctgtgcg gaccgtggct tgagccatct ctcagtcctc tctattgcgg aggagggcga
     1021 aattggcgag gctgatcacc gctaggcaga aggccagcac gtagctcatc gccgccgcgc
     1081 tgcccatatt gaagttctga agcccctctt ccgcgaggta catgatggcc gggcgggtat
     1141 cgtcgcccgg gccgccctgg gtaatgaggt aggcctggcc gaacatgttc gcggaggcca
     1201 gtatcgtcac gacgatgacg aagagcagga tcggccgcag ccccggcaga gtcacgtgca
     1261 agaacctccg ccaccggttc gcgccatcca ccttggccgc gtcgtagagc tgcggatcta
     1321 tgtcctggag gccggccagg tagatgaccg agttgaaccc gagcgtccac cagaccgtag
     1381 gcccgaccag ggcgatccag gcccagggca aagcgttcgt ccagggtatg ttgtcgggta
     1441 atcccagttg ccccaggtag tagttcacga gtcccacgtt cgcatcgagc aggtaccgcc
     1501 acaggatgcc gataacggcc acgctcagca cgtagggcgc gaagtacacg gtccggaaga
     1561 acgcccgtcc gcgaaaactc tgattgagca tcagggccac cagtaatggt atcaccacga
     1621 gcagcggtac ggagtagagc gtgaacttgc ccgtagcctc catgctctgc cagaagggtc
     1681 cgctggtagt cgaaccgggg gtaaacaggt cggtgtaatt ctcgagtcca acccagggct
     1741 tgccaggcag gaggtagtcc cagttgtgga ggctgatcca caccccgaag aaaaccggat
     1801 aaaggatgaa gaccccaagc aacaccaggt aaggcgccat gaacagatac ggcgttacgg
     1861 ggtgagggtc ggttggcttt cgcgcaccga caagggtttt gctcaccggt ggccgagcct
     1921 cgctcccctc gacattttgc gtggccacgg ttcgctacgc ctggtacttc tgacggttct
     1981 cctccaggag ttgtttggcc tggctcgctg actcatccag cgccgtcttc gggtccttct
     2041 tgagcaggag tgcctcgttg acggccgggt ccagggcgtt gagctggacg tcaccgatgc
     2101 caggcaccga aggaggaaag cgcacgtacg ggagctgctc ggcgaggttg ctctgaaact
     2161 cgagtttctg gaactccggg ctctcgcgga cgctgtttcg cgccggcacc tgaccggcct
     2221 tggcccactc cgcggagtga gtgctcaccc agttgatgaa cactttggaa gccgtgatct
     2281 tgttggggtc ctggccccgc ttgttggtga tcacgaagtt atgcgagttg gcccaagtcg
     2341 ccttctgcga acctatctga ggcaccggga cggcaccata gttcacacct gcctctttca
     2401 gcgggtttat attccagata ccgctccagt agaaggcgtt gttgttgttc tggaaggcta
     2461 tcagctcggc tccatcgccc acgttcttgg ggctatatcc ttgctcgatc aagtccacca
     2521 gccacgtgag tgcatccacg ccggcgtcgg agttccaggt cgccgcggtc acgtcctcgt
     2581 tgtacagctc gccgccgtac tgccagagca gcgtctcgaa gatccacgtc gatccggccg
     2641 ggaccgtggg cacccagaag ccctgcactc ctttcccttt tagctcctcc agcgccgcca
     2701 tgaactcgtc ttttgtctgg ggcggcttgt tcggatccaa cccacccttc tccatgacgt
     2761 ccttgttgta gaagtcgccg aacgggtgca cgtccagagg tatcccgtac cgcgagccct
     2821 cgtagatccc cgcgttccac acgatcggga cgaagtcgct ctccttcagc ttcaacgcgt
     2881 tggccacgtc gtcgagaggg atgatcacac tccgcgcggc gttggtagcc acggtgtcga
     2941 tgtgcattat ccctacgtca ggaccttctc cagtcgagac tgaagcggga actttctggt
     3001 agtaggtctc ccattcgact gtgttcatgg tgacgtcgat gttgtcgtgc tcggagctga
     3061 actgctccac gaggtcgcgc atcaaggggc cgtccccacc ggtgaagccg ttccagaacg
     3121 ccagatccac cttcggcccg ttgtactcct tgccaccgcc tccgccaccc tgctgctgca
     3181 cggcgggatt gttctccgga ccaccgccac aacccgccag catgagcccc gccccggcac
     3241 ccgcgccgag tagcaggaaa tcctgccgag tcagcctgcg gcctctcgca cgagttccgt
     3301 ccacgccttc ctccttctgc cgcaccccta ccgaacgtcc cctaccgctc tcgatgaccg
     3361 taaacccacg gtaacagcgg tctaatcacg ggtcaacctt aactaacagg aaacatgttg
     3421 actttgtttc gcacgccgtc ctacgcaggc tgcagacctt tcggcaacct gtgatccgga
     3481 catatgtgca catgcgtcgg agtggcgacc acgatgactc gtcgcgggct aacgcgtctt
     3541 caaccacacg cgcatcgacc cttgctcgcg gttcgcccag gcgaagtaag gtactgctgt
     3601 gagcttcgta gcgcctggct tgatgttgcg ctcgcgccga tgggcgttac ggtagaggcg
     3661 tccttcccac ccatcatcgg gttcgacgtt ctgtgcgccg acttccagca ctaccacgcc
     3721 gccgagcagg tcgggctcga agcgggtgga gggatcttcg tcttccagga caagcatgtg
     3781 caggtcgacg tcggggttat ctgtctgttc cgcgcagtag aggatggggc cgcgcatgag
     3841 cgcgacgcgg ccctcgttct cggcgacgta gggatggcat gcgacgcggc ggaccagcat
     3901 cgggaggttc atgctaacag tgtcgcctgg acgccacacg cggcgtatcg gcaggtacgt
     3961 tccgggagag acctcctccc cgagcggctc gccgtttacc tggaccgatg cgccctcctc
     4021 gcaccaggaa ggtatccgga gcatcatggt gaattcgcca tcgccttcta cggtgacctc
     4081 gaccccgcca tcccaggggt atccggtacg ctgccggagc ctgacgaccc tgttccggtc
     4141 cagttcgacc gtcgcgacgc cttcggcgta gaggtgtacc cagacggcgt cttcggaggc
     4201 accgtagaag tagcccggta gcgaggccag cagacgggcc acgttgggcg ggcagcaggc
     4261 gcagccaaac cacggctggc ggcggtgctt cccttcgtcg gcgagagggt tctggtagaa
     4321 gtagtgtcct ccgtcgaggg agacgcctag caggacggca ttgtagagag tgtgttcgat
     4381 caggtcggcg tacctggcgt ctcctcctag catgagcatg cgccagttcc acatcacgct
     4441 gccgatggct gcgcacgtct ccgcgtaggc gcgttcgttg ggaagctcgt aatcgtcgcc
     4501 aaacgcttca ccctcgtagc gagatcccag gccgccgctc acgtacatgc ggcgggtggt
     4561 catgttgcgc cacaggttct gcagcgccgc gagtagggcg ccatcaccaa tctcggcgta
     4621 aacgtcagcg gccccgcagt agaggtacat ggcacggact gcgtggccga cggtctcact
     4681 ctgctcgcgg aggggttcgt ggtcctggct gtaggagggc tcgtgtagtc cgtagggtct
     4741 gccgagaaag ccgtgtccgc gcgcgtcgac gaagaaccgc gcctgttcca ggtagcgccg
     4801 gttgcccgtg gccctgaata gttcgaccag ggccatctcg atctcctcgt gcccgtcgac
     4861 ggcgacccgc ttgccctgct catcctggcc gaacacgtcg cagatgtgat ccgcgaacct
     4921 cacggctact ccgagcaggc gctccgagcc ggtcgcgtag aagtgggcca cggccgcctg
     4981 gaagagatgg cctgcgcagt acatctcgtg caagtcgaag tccgtccagc gctcggacgc
     5041 cctctcaaag gtaaagtacg tgttcaggta accgtccgtc tgctgggcgt cttcgatctc
     5101 ggttatggca atgtcaatca tccgggtgag ttcagggtcc ggtccttcgg cgagagacca
     5161 cgacgacgcc tccagccatt tgtacacgtc cgagtcgttg aaatagaggc cgcggaacgg
     5221 cgcgtcgatc ttgcccgatg cgcgtctgaa gttgtccagc cgtccggtct cctcgatgtg
     5281 ctcgaactgc gagggcagcg tcttctcccg gttgatgcgc ctccgcggct cccagaagcc
     5341 gtcagagagc cgtacgtcgg gtaacgagag aggtcgcagt ctggcgtgtc ggctgcggga
     5401 ggtatcaacc acaacaggcg ggcgtggcat cgagtcgagc attgtcctcc ttatccggcg
     5461 agtccggcga cgaggaccgt gaccgagcac gcggggaagc tgtgctcgaa agaacggcct
     5521 tcagttcgta cggagcgctc gctcacccct accgtctgat ggtcgaagtc gtttataccc
     5581 gccgggtcct tgcccgtcac ctcgtagacc gtggcattgc cctcaaaggt cgagtcggcg
     5641 agctggatcg tcgtctgcat cgcttgctcc gggtctcggt tgacgacgac gagcgtcaga
     5701 gagcggcctt ctgcatcgca ggtcgccgac acgtcgagca ggtcgaaggg tcccatgtcg
     5761 gcgacgcggt gcggccatga agactcctcc gtggtcaggt catgcttttc ggaggcgacg
     5821 tgggcgtcga gcaccacgtc gtgcatatgc tcggcgcaca gcctgagggg atgatagatc
     5881 gtctgcaaga acaggccttc ctcgttggta aagatcggtg ctatcacgtt gaccatctgg
     5941 gcgaggttgg cgatcttgac tgtctcgcag tggcgggcga agacgttgag gtatgtggcg
     6001 acagcgaggg cgtcggacag gttgtagcgt tcttcgaggc ccgaggtctg ctctcgttcg
     6061 cggaaccaaa cgttccactc gtcgtaggcg acgtggatgg gatggtcgat gcgttgctcg
     6121 taacgggcgc gttcgatcat ggcccgcgtc gtgcgaagcg cgcggtcggc ctggtgaggg
     6181 gcgagtacgt tcgaccagta gtcgtcgctg cccgtatata tgtgaatgct gtgatagtcc
     6241 acgaacgggg cgagaccctc gataactacc ctgtcccaat ccgtcagccc gctcaggcca
     6301 caaccaacga gctctatatt cgggtcagtc cacctcatca ccttcgcgaa ctcgcgggcc
     6361 ttcctcacgt actcttccgc ggtgagcgcc ccgatctgcc actccccgta catctcgttg
     6421 ccgaggcccc agtacttgac g
//
LOCUS       DATAIR010000225         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_429244, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000225 DATAIR010000000
VERSION     DATAIR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429244"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHM69_05395"
     CDS             <1..222
                     /locus_tag="VHM69_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739861.1"
                     /translation="VPQGARDVRSFYAGSTLQQRMQILRHYNPDFVLVHANSPPERQL
                     QHLPGFTDLNAPGKRYSLLAVDAERLPSP"
     gene            281..718
                     /locus_tag="VHM69_05400"
     CDS             281..718
                     /locus_tag="VHM69_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX2739862.1"
                     /translation="MRGAVLAMRKVGAVDIEAVLKRGEGITKNLGVEFVELTRERVVA
                     TMPVDERHHQPLGYLHGGVSVVLAESVASTGAFLNCPPGKVAFGSEISASHVRPKQSG
                     TLTAVAVPAHVGRRSQVWEVRITDEDDKTVCVSRCTVAVVDGG"
     gene            715..1317
                     /locus_tag="VHM69_05405"
     CDS             715..1317
                     /locus_tag="VHM69_05405"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2739863.1"
                     /translation="MMKKGERTRRRILERSAPVFNTKGYFGASMNDLVLASGLEKGGI
                     YNHFQSKEQLALEAFDYAVEVMSGRFRETLAGREGAIERLFAIVDVLGALADDPPVPG
                     GCPVLNTAVEADGANPALKERAAEAMDGWLRLVGREVKEGVSRREISSGTDPRAVASV
                     VVATLEGAVMLSRLEDDPEHMRRAVKHLKDHLRSLAKEDV"
     gene            1314..1847
                     /locus_tag="VHM69_05410"
     CDS             1314..1847
                     /locus_tag="VHM69_05410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2739864.1"
                     /translation="MKGKIGRTRTISWEDPAPAAEAARKMGGLEFLRAIMRRELPAPP
                     MADLMDFGLVEVDEGRVVFECTPAEYHYNPIGAVHGGFACTLFDSAMGCAVHTELPEG
                     AGYTTVELKVNLLRPITAETGRLLCEGESIHVGRRIATAEARLKDESGRLYGHATTTC
                     MVFREGSKERSVGEGGG"
     gene            complement(1860..2816)
                     /locus_tag="VHM69_05415"
     CDS             complement(1860..2816)
                     /locus_tag="VHM69_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739865.1"
                     /translation="MKLRTAVMVGGGIWGLALYNRRLERSGGELPNRLGGETRYYGWK
                     GGDLAYSVAGEGEPLLLVHGVYAGASSFEFRKNFEELSRSFRVYALDLLGCGMSEKPG
                     RRYEPEDVTSQVEDFVREEIGGQAHLVASSLSAALVVPAAVRNPRLFKKLVLICPTGF
                     GTLDRPSGRLGDVIYRLFMAPVLGKALYHAIVSRLGIRYYLGSMAYHDPGLVTEGIVE
                     GYYRTSHQLGASYFPAAFVSGKLNLGVAGLWPRVPHRTLICWGIDAKTVPVNEAERFV
                     QHNPRSEPRIFKDSALLPHDERADTFNEEVKKFLLKKTGSRG"
     gene            2954..>3346
                     /locus_tag="VHM69_05420"
     CDS             2954..>3346
                     /locus_tag="VHM69_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX2739866.1"
                     /translation="MDRDLLLDETLMLLPTLMRLVERPSPVELGEIERRGGPKEDVQV
                     SPGHIQVLIALTGGPRSVGQLAEELDVSPPAATQLVDKLAEHGMVDRHHDPSDGRVVL
                     VDYVDGMHDVARRIIEDRRHHLQDALSKM"
BASE COUNT          667 a          999 c         1094 g          586 t
ORIGIN      
        1 gtgccccagg gcgcgcggga cgtgcgtagc ttctacgccg gatccaccct ccagcagaga
       61 atgcagatcc tgcgccacta caaccccgac ttcgtgctgg tccatgcgaa ctcaccaccg
      121 gaaagacaac ttcaacacct ccccggcttc acggacctca atgccccagg gaagagatat
      181 agcctgctcg ccgtggatgc agagaggctc cccagcccgt aacactacga accccgccac
      241 aaaaccgacc gattggtctt aaacgaagag atcgggtaat atgcgtgggg ctgttctggc
      301 gatgcggaag gtaggggccg tggacataga agccgttctc aagagggggg aaggtatcac
      361 caagaatctg ggcgtcgagt tcgtggagct cacacgggag cgggtggtgg cgacgatgcc
      421 cgtggacgag aggcaccacc agccgctcgg gtatctgcac ggtggggtga gcgtggtatt
      481 ggctgagagc gtggcgagca cgggggcgtt cttgaactgt ccgccgggga aggtggcgtt
      541 cggatcggag atcagcgcga gccacgtcag gccaaagcaa agcgggacgc tcacggcggt
      601 cgcggtaccc gcgcacgtcg ggcgcaggag ccaggtatgg gaagtccgca tcacggacga
      661 ggacgacaaa acggtatgcg tctcgcgctg caccgtcgcg gtcgtggacg gcggatgatg
      721 aagaaaggcg agaggacgcg gcgcaggatc ctggagcgtt ccgcgccggt cttcaacacg
      781 aagggctact tcggggcctc gatgaacgac ctcgtgctcg catcgggcct ggagaagggc
      841 ggcatataca accacttcca aagcaaggag caactggcgc tcgaggcctt cgattacgcg
      901 gtggaagtaa tgagcgggcg tttcagggaa accctcgccg gcagggaggg tgcgatcgag
      961 cgtctcttcg cgatcgttga cgtgctgggc gccctcgccg atgacccgcc cgttcccggc
     1021 ggttgtccgg tcctcaatac ggccgttgag gctgatggtg ccaaccctgc gctcaaagag
     1081 agggcggctg aggcgatgga cggctggttg cggctcgtcg ggcgagaggt caaggagggc
     1141 gtaagtagaa gggagatttc gtccgggacc gatccgaggg ccgtcgcgag cgtggtcgtg
     1201 gcgacgctcg aaggggctgt gatgttgagc aggctcgaag acgacccgga acacatgaga
     1261 agggccgtga agcatctcaa ggaccatctg cgttctctgg caaaggagga cgtatgaaag
     1321 ggaagatcgg acgcacccgc actatcagct gggaggaccc cgcgccggct gctgaggcgg
     1381 ccaggaagat gggcgggctc gagttcctgc gggcgataat gcggcgggag ctgcctgcgc
     1441 cgccgatggc ggatctcatg gacttcggac tcgtcgaggt cgatgagggg cgcgtagtct
     1501 tcgagtgcac gcccgccgag taccactaca atcccatagg cgccgttcac ggtggtttcg
     1561 cctgcacgct cttcgactcg gccatgggct gcgccgtgca tacagagtta cctgaaggcg
     1621 caggctacac taccgtcgag ctcaaagtta acctgctgcg tccgatcacg gccgagaccg
     1681 gacgccttct ttgcgagggt gagagtatcc acgtcggtcg acgcatcgcc accgccgagg
     1741 cccgcctgaa ggatgaatcc ggcaggctct acgggcacgc cacgacgacg tgcatggtgt
     1801 tccgcgaagg ctcgaaagaa cgctccgtcg gtgaaggtgg aggatgacgg ggcgtcgccc
     1861 tagccccggc ttcccgtctt ctttaatagg aacttcttca cctcttcgtt gaacgtatcc
     1921 gcccgctcgt cgtgtggcaa gagggccgag tctttgaaga tgcgcggctc cgagcggggg
     1981 ttgtgctgaa cgaaacgctc cgcttcattc accgggactg ttttcgcatc gatgccccag
     2041 cagatcaggg tccggtgcgg cacccgcggc cataggccgg ctaccccgag gttgagcttg
     2101 cccgagacga aggccgcggg gaagtaactc gccccaagtt ggtggctggt cctgtaataa
     2161 ccctcgacaa tgccttccgt cacgagtcca ggatcgtggt acgccatgct cccgaggtag
     2221 taccggatgc ccaacctgga gacgatcgcg tggtatagcg ctttcccgag gacaggcgcc
     2281 atgaagaggc ggtagatcac atcgcccaga cgccccgagg gacggtcgag ggtcccgaag
     2341 cccgtcggac agatcaaaac gagcttcttg aagagccggg ggttacgtac ggccgccggc
     2401 acgacgagcg cagctgagag cgagctggct acgaggtgcg cctgcccccc gatctcctcc
     2461 cgcacgaagt cctcgacctg cgatgtaacg tcctcaggct cgtagcgcct ccccggcttc
     2521 tccgacatgc cgcacccgag cagatccagg gcatacaccc tgaagcttct cgacaactcc
     2581 tcgaagttct tgcggaactc gaaggaagac gcacccgcat acacgccgtg taccagaagc
     2641 agcggctcac cttccccggc tactgaatac gcgagatccc cacccttcca cccgtagtag
     2701 cgcgtctcgc cgccgagccg gttcggaagt tccccgccgc tcctctccag tcgccggttg
     2761 tagagcgcga gcccccagat ccccccgcct accatcaccg ccgtcctcaa cttcatcctt
     2821 ctacctccaa atctctcgac gcccagggga tcctacctga aagcgcccgc cagattgcgt
     2881 cttgacaaac cgcgaacaca ggattacatt taagtaggtt atataagtag cttaaatttg
     2941 taggagaatt cacatggaca gggatctgct cctcgacgag acattgatgt tgttacctac
     3001 gttgatgcgg ctagtcgagc ggccaagtcc tgtggagttg ggtgagatcg agcgccgtgg
     3061 aggaccgaag gaggatgtcc aggtatcgcc tggtcacatt caggttctga tcgcgcttac
     3121 gggcgggccg cggtcggtgg ggcagttggc agaggagctt gacgtctcgc cgccggctgc
     3181 gacgcagttg gtggataagc tggccgagca cggaatggta gaccgtcacc acgatccgtc
     3241 ggacgggcgg gtggtgctcg ttgattacgt ggacgggatg cacgatgtgg cgcggcgcat
     3301 tatcgaggac cgccgccacc accttcagga tgcgctgagc aagatg
//
LOCUS       DATAIR010000226         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_291292, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000226 DATAIR010000000
VERSION     DATAIR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291292"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="VHM69_05425"
     CDS             <1..2397
                     /locus_tag="VHM69_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2739867.1"
                     /translation="TFKTFMESTHPEDRAYVDKIIRGAASKREPSLSFEHRILRPDGE
                     VRFVQVWMEFVYGEDGEAIKSFGTVLDITERKQAEEALRESERDLLAAQRIASVGNWS
                     FDVAENRAHWSEEMYRIFGLTPQERLTFRRFLSHVHPEDRRHIVEAAREALKGDGRTG
                     LDYRAVRPDGEVRYVHAQYEVERDASGWATQLIGTLQDITGLRRTEAELRKSEERFRS
                     LVQNSLDVITVVDARGTIVYYSPSAERVMGYRPEEFIGKDALEESQIHPEDFPRVRDI
                     FSYLVENPGVNYSMELRMRHADGSWRVIEAMGNNLLDDPSVNGIVINSRDVTERQAFE
                     EPLSPQPFHDPRTDLPNRALLMDRLEHALARADRHPESIAVLFLDLDNFKVINDSLGH
                     KAGDELLVATAGRLRRCLRPGDTVARLSGDEFTILLEDMTDRKDAIRLAERIAERMDK
                     PFTLKGRDAFTAASIGIAFAASSADRPDDLLRKADIAMYRAKSRGKAGHIVFDPGMDA
                     EVHKRLRMESDLRRAVEREEFVLHYQVKARLDTSVQRHIRSTRSPALVTPTGADGARA
                     VEVEALVRWKHPRHGLVLPMEFIPLAEETGLIIPMGRWILAEACRQARSWREQSAEDL
                     PHTVCVNLSARQFQHPKLVEEVGEVLREVGLDPGSLMLEITESTLIEDIKSATTTLQQ
                     LRDLGVKLAVDDFGTGYSSLSYLKRFPVDFLKIDLSFVENLETDPADREIVSAVINLA
                     HALGMKVTAEGVETAGQLAQLTSMGCDMAQGFYFSKPLPSEEIRSLLSSPLTDGVSPR
                     "
     gene            complement(2420..>2837)
                     /locus_tag="VHM69_05430"
     CDS             complement(2420..>2837)
                     /locus_tag="VHM69_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2739868.1"
                     /translation="IPVPVISAIIGEGCSGGALAMCATDYVGMLENSYLSVIAPEACA
                     SIIFRDPSRASEAAEALKLTATDMKGRGIVDEVLPEPLGGAHNDPEAAIETVGKALNR
                     VLTGLSDADPDTLVQARYERYRRIGAHTAIGEDPSG"
BASE COUNT          584 a          846 c          906 g          501 t
ORIGIN      
        1 acgttcaaaa ccttcatgga atccacccac cccgaagaca gggcgtacgt ggataagatc
       61 ataagaggcg ccgcatccaa aagagagcct tccttatctt tcgagcaccg catactgcgt
      121 ccggacgggg aggtgcgctt cgtccaggtg tggatggagt tcgtctacgg cgaggatggt
      181 gaagcgatca aaagcttcgg tacggtcctc gacatcacgg agcgcaagca agcagaggag
      241 gcgctcaggg agagcgagag agatctcctc gccgcccaga ggatagccag cgtcggaaac
      301 tggagcttcg atgtggcgga gaaccgtgcc cactggtcag aggagatgta caggatcttc
      361 ggcctgactc cacaagagcg actcacgttc aggaggttcc tctcccacgt ccatccggag
      421 gaccgcaggc acatcgtgga ggctgcccgc gaagctttga aaggagatgg tcggaccggc
      481 ctggattacc gggctgtcag gcctgatggc gaagtgcgct acgtacacgc gcagtacgag
      541 gtggagcgcg atgcctccgg gtgggcgacg caactcatcg gtacgctcca ggacatcaca
      601 ggactgcgaa ggacggaggc ggagctacgc aagagcgagg agcggttccg ctcgctcgtc
      661 cagaactcgc tcgatgtgat cacggtggtc gatgctagag ggacgatcgt gtactacagc
      721 ccctccgccg agagggtcat gggctacaga cccgaggagt tcatcggcaa ggacgcgcta
      781 gaggagtccc agatccatcc ggaagacttc ccaagggtgc gagacatctt cagctacctg
      841 gtcgaaaacc ctggggtcaa ctactcgatg gagctgcgga tgcggcacgc tgatggctcg
      901 tggcgcgtga tcgaggctat gggcaacaac ctgctcgacg accccagtgt caacggtatc
      961 gtgatcaact cccgcgacgt tacggagcgc caggcttttg aggagccgct atctccccag
     1021 cctttccacg atcctcggac ggatcttcca aaccgcgccc tactcatgga ccgcctcgag
     1081 cacgccctgg cccgcgccga ccgccacccg gaatccatcg ccgtcctgtt cctcgacctg
     1141 gacaatttca aggtcataaa cgactccctc ggccacaagg cgggagacga gctgctcgtc
     1201 gccaccgccg ggcgcctgcg gcgttgtctg cgccccggag ataccgtggc acgcctcagc
     1261 ggggatgagt tcacgatcct gctcgaagac atgacggacc ggaaggacgc tattcgcctg
     1321 gcggaacgga tcgcggaacg gatggataaa ccgttcaccc taaaaggtcg cgacgcgttc
     1381 accgcggcca gcatcgggat agctttcgcc gcttccagcg ccgacaggcc cgatgacctc
     1441 ttgcgcaagg cggacatcgc catgtaccgg gccaagagca ggggtaaggc tggacacata
     1501 gtgttcgacc cgggcatgga cgcggaggtc cacaaaagac tccgtatgga gagtgacctg
     1561 cggcgggccg tcgagcgcga ggagttcgtt ctccactatc aggtgaaggc gcggctggac
     1621 acgagcgtgc agcggcacat caggtccacc cgaagtcccg ccctcgtgac accgacggga
     1681 gcggatggag ccagggccgt ggaggtcgag gcgctggtgc gctggaagca tccccgtcac
     1741 gggttggtgc ttccgatgga gttcattccc ctcgctgagg agaccggttt gatcatcccc
     1801 atggggcgtt ggatactggc ggaagcctgc aggcaggccc gcagctggcg ggaacaatca
     1861 gccgaagacc tgccccatac cgtgtgcgta aacctctccg cacgacagtt ccagcatccg
     1921 aaactggtcg aggaggtcgg cgaggttctg cgagaggtag ggttggatcc cggtagcctg
     1981 atgctggaga tcacagagag tacgctgata gaagacatca aatccgccac caccacactt
     2041 caacaactga gagatttagg cgtgaagctg gcggtggatg acttcggtac gggctactcg
     2101 tccctctcat acctcaagcg cttccctgtc gattttctga aaatagatct ctcgttcgtg
     2161 gagaatctcg agacagaccc cgcggacagg gagatcgtct cggcggtgat caacctggca
     2221 cacgcccttg gcatgaaagt aaccgccgag ggcgtagaga ccgccggcca actggcacag
     2281 ttgacctcta tgggatgcga catggcccag ggcttctact tctcgaaacc gctcccgagc
     2341 gaggagatac gctccttact ctcatctcct ctcaccgatg gagtatcgcc acgctagatg
     2401 agagtaagga tcgttcagcc taaccggatg ggtcctctcc tatggcggtg tgcgcgccga
     2461 tgcggcggta gcgttcgtag cgggcctgga caagcgtgtc agggtcagcg tccgagaggc
     2521 ctgtgagcac gcggttcaga gctttgccca cggtctcgat cgctgcctcc gggtcgttgt
     2581 gggcgcctcc gaggggctcg gggaggactt cgtccacgat cccgcgtccc ttcatgtccg
     2641 tagcagtgag ctttagagct tcggcggctt ctgaagcccg gctggggtcg cggaagatga
     2701 tcgaggcgca cgcctcgggc gctatgacgg agaggtagga gttctctagc atcccgacgt
     2761 agtcggtggc gcacatggcg agcgctccgc cggaacagcc ctcgccgatg atggccgaga
     2821 tcacgggcac agggatc
//
LOCUS       DATAIR010000227         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_429476, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000227 DATAIR010000000
VERSION     DATAIR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429476"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VHM69_05435"
     CDS             <1..1317
                     /locus_tag="VHM69_05435"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEX2739869.1"
                     /translation="VLGPTSVANLDALRALLPWLRPRPLGIRASAGFGDRLGLATPGH
                     VRAMRAAGRGLAPIFAQQSIREMERTGRSPQEVLDDATWGVFAEGWREGFGADADHLK
                     TAEDVDACVAAGYTFFTFDPGEYVDDGAEAADASALISGLEDLPWDDLEDRPEDLKRR
                     YLDRAFGTEEYEVRFDDLSLARAAVKYGGAIAHVARLYRHLEAAMGDRDFEVEVSVDE
                     TESPTTHAQHVYIAGELRRLGVRWISLAPRYVGTFEKGVDFIGDVGQFEEDFAVHAAI
                     ARDDSSGGPYKLSLHSGSDKFSIYPAVTRQAGGLVHLKTAGTSYLEALRTAAGLDPRF
                     FREVYAFARDRYEEDRASYHVSASLKRAVSPEDVPDEELPVLLTRFDEREILHVTFGS
                     ILGERRLRERLFGLLHSHPEAYAAGLEAHFVRHLEPFAKGVQEEKT"
     gene            1393..>1960
                     /locus_tag="VHM69_05440"
     CDS             1393..>1960
                     /locus_tag="VHM69_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739870.1"
                     /translation="MRKDVEKGSGRSRDTPDAVEAVHPVEILRDAVESYSPHLQPSGE
                     LHDPVFGEPTQYGTPYHALCNAVLAMRDGGERRSAYVDRAARGLKAALDHVSDPGLPP
                     TASGFDPATGTVTRSNHRDFFWPPIIKTFRILRSMRVDGTEVFAQRISGVDIERAFAK
                     RPPSNWASVWLSGEWLRLREGLSPFSKED"
BASE COUNT          344 a          629 c          637 g          350 t
ORIGIN      
        1 gtgctgggtc cgactagcgt cgccaacctg gacgccctgc gcgccctcct gccctggctg
       61 cgccctcggc cgctcggcat acgagcctcg gcgggcttcg gtgacagact tgggctcgct
      121 acccccggtc acgtccgggc aatgcgcgcc gccgggagag gactggcgcc catcttcgcc
      181 cagcagtcga tccgggagat ggaacgtacg gggcgcagcc ctcaggaagt cctcgacgac
      241 gcaacgtggg gcgtcttcgc agagggctgg agggagggct tcggagcgga cgccgaccac
      301 ctcaagaccg ctgaagacgt ggacgcgtgc gtcgctgccg gctatacgtt ctttaccttc
      361 gatcccggag aatacgtcga cgacggggct gaggccgccg acgcttccgc cttgatctcc
      421 ggtctagaag atttgccgtg ggacgacctc gaagaccgtc ctgaggacct gaaacgacgc
      481 tacctcgaca gagcgttcgg gacggaggag tacgaggttc gcttcgacga tctctccctg
      541 gctcgggcag ccgtcaaata cggtggcgcc atcgcgcatg tggcccgact ttaccgccat
      601 ctcgaggccg cgatggggga cagggacttc gaggtcgagg tctccgttga cgagacagag
      661 agcccgacga cgcacgccca gcacgtctac atagccggcg agttgcggcg tctaggcgtg
      721 aggtggatca gcctggcacc gcgctacgtc ggtaccttcg agaagggcgt ggacttcatc
      781 ggggacgtcg gacaattcga ggaggacttc gcagtccacg cggccatcgc ccgcgatgac
      841 tcgtcgggtg ggccgtacaa gctgagtttg cactccggtt cggacaagtt ctcgatctac
      901 cctgccgtca cccgccaggc cggcggcctt gtccacctca agaccgcagg cacgagctac
      961 ctggaagcgt tgagaacggc cgccgggctc gaccctcgat tcttccgcga ggtgtacgct
     1021 ttcgcccgag atcgctacga agaggaccgc gcgagctacc acgtctcggc gagtctgaag
     1081 cgggccgtct cgcccgagga cgtgcccgac gaggagcttc ctgttctgct cacacgcttc
     1141 gacgagcgtg agatcctgca cgtcaccttc ggctcgattc tcggtgaaag gcggctgcga
     1201 gagcgcctct tcgggctgtt gcactcccac cccgaagcct acgccgccgg cctggaggcc
     1261 catttcgtgc gccatctgga gccctttgcc aaaggtgttc aggaggagaa gacttgatcc
     1321 gccttcaggt ggaaggagag tggatcggag gtgccattca cccgacgcgc ctggccgcga
     1381 agaggtagga acgtgcggaa agatgttgaa aagggaagcg ggcggagtcg cgatacgccg
     1441 gatgctgtgg aagcagtcca tccggtcgag atcttgaggg acgccgtcga gagttactcg
     1501 ccccacctgc agccttcggg agagttgcac gatccggtct tcggtgaacc gacgcagtac
     1561 gggactcctt atcacgccct ttgcaacgct gtgcttgcca tgagggacgg tggtgagaga
     1621 cgttcggcct acgttgaccg cgccgctcgc ggtctgaagg cagcccttga ccatgtctcg
     1681 gaccccggcc tcccgccaac cgcttccggc ttcgacccgg cgacgggcac ggtgacccgc
     1741 agcaaccaca gggacttctt ctggccgccg atcatcaaga cgtttcgcat cctcaggagc
     1801 atgagggtgg acggcaccga agtctttgca caacggatct ccggggtgga catcgagcgc
     1861 gctttcgcca aacgacctcc ctcgaactgg gcctccgtct ggctctcggg cgagtggctc
     1921 cgtcttcgtg aaggcctctc tccgttctcg aaggaagata
//
LOCUS       DATAIR010000228         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_329425, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000228 DATAIR010000000
VERSION     DATAIR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329425"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..625
                     /locus_tag="VHM69_05445"
     CDS             179..625
                     /locus_tag="VHM69_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX2739871.1"
                     /translation="MALAISKITVLAMLAGGNVFTGNWGGVSYGGVEEVGNNGVRHST
                     NTGGVPQQEIAPATTMLASYYGREFEGQPMASGQPYDADAYTAAHKSLPFGTQLQVSY
                     GGESVRVTVMDRGPYVAGRDLDLSLAAAQELGVTVPGEAPVRVRVL"
     gene            complement(638..1432)
                     /locus_tag="VHM69_05450"
     CDS             complement(638..1432)
                     /locus_tag="VHM69_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739872.1"
                     /translation="MADVNGARIYYEVAGEGEPLVLVHAGIADSRMWEAQLAEFANHY
                     QVIRYDMRGFGRTAMVEGPYSHHDDLRGLLDSLDVGRTYLVGCSMGGGAVLDFALEFP
                     ERSGNLVLVGPAVGGFRPDFDPPRQWDELVAADEAGDLGRISELEVQIWVDGPKRSPE
                     DIEASIRDLVREMNLIALENEASGLGEEWEPEPPTVDRLPDIQAPSLLIVGDEDQPRV
                     FAAADLLERELPNVRRVVIPGTAHLPNMERPEHFNRLVLDFLEDFR"
     gene            1648..2472
                     /locus_tag="VHM69_05455"
     CDS             1648..2472
                     /locus_tag="VHM69_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX2739873.1"
                     /translation="MALTVESGPFGERSKGTFNFDTTLLQPHTLYFEDSPRRVRVMFG
                     GETVADSRHVKLLHETGHLPIYYFPEEDVRMDLLEATDHTTYCPFKGDASYWSVRVGD
                     RVAENAVWSYAEPIEPCPPIGGHLAFFWNAMDHWFEEDEEVFVHARDPYHRVDVLQSS
                     RHVRVSVNGEVVAETDRPKILFETGLPPRYYIPPEDVREEVLAGSEKTTGCPYKGVAS
                     YYSIQAGGDRVDDLVWYYPEPIPEAAKIEGHLAFFNEQVDIEVDGEAQERPRTRWS"
     gene            complement(2544..>3680)
                     /locus_tag="VHM69_05460"
     CDS             complement(2544..>3680)
                     /locus_tag="VHM69_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739874.1"
                     /translation="RAYSEDYDVPTSAERSTMAAAYDAIEAGDLSQAASLADPLDYDV
                     VRYEDTSNGRTLIMLSERSTEDAFGKKIWPHAWGMYIFSPHAGSDITVEVTHPVFDSR
                     TEDFGLAVFQKANAEDLLIAGAHRYANCDEQQPCSSPDPNAAADVAHAPDSVFEGIHE
                     AAVESPTNVFQPHGFNSANHQGEDCGEVFVSAGKTPPSPLAEEVHHTLGNVGFTARLF
                     GAADGTTCKGLGATTNVQGTWTNQIGADFLHTEMRESIRRDPDDPNYDPIRESLLTST
                     IADSLLPPTVTSLRPAPGSITRDRTPAVGATVRDAHTDLTKANVRLSVDGRQILTFTY
                     DQGTDKLSYTPGRNLSLGGHKIRVQATDGAGNSATRRWGFKVAR"
BASE COUNT          683 a         1124 c         1144 g          729 t
ORIGIN      
        1 gtcgtcaccg gccatgtgct tcctcctctt ccaggactcg cccacatact acgcgaccga
       61 ctcccgaacg ttcctgatat gtgagtttgg acactacaag ctcgcaacaa tgtgttacat
      121 tcccccgctt acgctgaaag agaatacttg aagtagacca cgacatgctg gaggggtttt
      181 ggcattagcg atctcgaaaa taacagtcct ggcgatgctc gctggaggca acgtgttcac
      241 aggtaactgg ggcggagtga gctatggcgg cgtggaagaa gtcggcaaca acggcgtccg
      301 tcactctact aatacgggcg gcgtccctca acaggagata gcgcctgcga ctacgatgct
      361 tgcctcctac tacgggcgcg agttcgaggg tcagccgatg gctagcgggc aaccctacga
      421 cgctgacgcc tacacggccg cccacaagag tctgcctttc gggacgcagc tgcaggttag
      481 ctacggcggc gagtcggtgc gggtaaccgt catggacagg gggccctacg tggcgggccg
      541 cgacctcgac ctctcgctgg ccgcggcgca ggagctcggt gtgacagttc cgggcgaggc
      601 gcctgtccgg gtcagggtac tatagacccg gcgcgcgtta tctgaagtct tcaaggaaat
      661 ccaacacgag cctgttgaag tgctcgggcc gctccatgtt gggcaggtgg gctgtgcctg
      721 gtatcaccac cctgcgcacg ttcggtagct ccctttcgag caggtcggcg gccgcgaaga
      781 ccctcggctg gtcctcgtca ccgactatca gcagggatgg agcctggata tcggggagcc
      841 ggtccaccgt gggaggttcc ggttcccact cctcgccaag ccccgaggct tcgttttcga
      901 gggcgatcag gttcatctcc cgaacgaggt cccggattga agcttctatg tcctcaggag
      961 acctcttggg gccgtctacc cagatctgga cctcgagctc ggatatccgc ccgaggtctc
     1021 cggcctcgtc cgcggcgacg agctcgtccc actgcctcgg ggggtcgaaa tccggcctga
     1081 acccgcccac tgccggccca actagtacga gattcccaga tctttcaggg aactccagag
     1141 cgaagtcgag cacggcccct ccccccatcg agcagccgac gagatacgtc cgcccgacgt
     1201 ccagagagtc gagcagaccg cgcaggtcat cgtggtggga gtacggcccc tccaccatcg
     1261 ccgtcctgcc aaagccacgc atgtcgtaac ggataacctg gtagtggttc gcgaactccg
     1321 cgagttgggc ttcccacatc ctgctgtcag cgatccctgc gtgcacgagg acgagaggct
     1381 caccttcgcc cgcgacctcg tagtagatcc tcgcgccgtt gacatcggcc attcctgtct
     1441 cccgaagcat cacttccctt tccacgatac ggtttcgccc acgtcgagct tcttcccggc
     1501 tggccggggt actgttctgt ttctttggaa cactcttttg aagacgcatc ggtggcaccg
     1561 gcagtatacg ccacccgaga cacaggaggg ccgtccggag catcgggtat aaagtatggt
     1621 cgggacgacg catgagagga gcaagagatg gcattgacgg tcgagagcgg gcctttcggt
     1681 gagcggagca agggcacgtt caacttcgac acgacgttgc tacagccgca cacactgtac
     1741 ttcgaggact ccccgcgcag ggtgcgggtg atgttcggtg gagagaccgt cgccgatagc
     1801 cggcacgtga agctcctgca cgagacggga cacctgccga tctactactt ccccgaggag
     1861 gacgtgcgta tggaccttct cgaggccacg gaccacacga cgtactgtcc cttcaagggc
     1921 gacgcctcgt actggtctgt gcgggttggc gaccgcgtcg ccgagaacgc cgtgtggagc
     1981 tatgctgagc cgatcgaacc ctgcccgccc atcggggggc acctcgcgtt cttctggaat
     2041 gcgatggatc attggttcga agaggacgag gaggtcttcg tccatgcccg tgacccttac
     2101 catcgggtgg acgttcttca gagctcgcgg cacgtcaggg tctccgttaa cggcgaggta
     2161 gtagccgaaa ccgatcggcc caagatcctg ttcgagaccg gactacctcc gcgctactac
     2221 atcccgcctg aggacgtgcg cgaggaggtg ctcgcaggga gcgagaagac gaccggatgt
     2281 ccgtacaaag gcgtcgcctc gtactactct atccaggccg ggggagatag ggtcgatgat
     2341 ctcgtctggt actacccgga gcctataccg gaggcagcca agatagaggg gcatctggcc
     2401 ttcttcaacg agcaggtgga catcgaggtg gacggcgagg cccaggagcg ccccaggacg
     2461 cgctggtcgt agccctaccg gacctgccac cgcccggttc ttcgggcaag gtcggcagta
     2521 tctcctttct ggttcacagc cttctaacgc gccactttga agccccaccg cctggtcgcg
     2581 ctgttgcccg caccgtccgt ggcttgcacc ctgatcttgt gaccacctag agaaaggttc
     2641 ctccccggtg tatagctcag cttgtcagtg ccctggtcgt aggtgaaggt cagtatctgc
     2701 cggccgtcga cggacagccg cacattggcc ttcgtcagat ccgtatgcgc gtccctcacg
     2761 gtcgctccca cggccggcgt gcggtctctg gtaatcgaac cgggagcggg gcgcaaagac
     2821 gtaacggtgg gcggtagcag ggaatccgcg atggtactgg tcaggagcga ttcacggatg
     2881 ggatcatagt tgggatcatc gggatcacga cgaatagact ccctcatctc ggtgtggaga
     2941 aagtccgccc cgatttggtt ggtccaggtt ccttgaacgt tggtggtcgc gccgagcccc
     3001 ttgcaggtag tgccgtccgc tgcgccgaac aaacgcgcgg tgaaaccaac gtttcccagg
     3061 gtgtgatgca cctcttctgc gagtggggat ggaggggtct tccccgctga gacgaagacc
     3121 tcgccgcagt cttctccctg gtggttcgcc gaattgaatc catgaggctg gaacacgttc
     3181 gtaggagatt cgaccgccgc ctcgtggatg ccctcgaaca cggaatcagg cgcgtgggcg
     3241 acatcggccg ctgcattggg gtccggcgag ctgcagggct gctgttcatc gcagttcgcg
     3301 taccgatggg ctcccgcgat gagaaggtcc tcggcgttcg ctttctggaa gacagcgagg
     3361 ccgaagtcct cggtgcgtga gtcaaacacg gggtgggtaa cctctacggt aatatcggat
     3421 cccgcgtgag gagagaatat atacataccc caggcgtgag gccagatctt tttcccgaag
     3481 gcgtcttccg ttgaccgctc ggaaagcata attagcgtcc taccgttgct cgtgtcctcg
     3541 tagcgtacga catcgtaatc gaggggatcc gcgagtgatg ccgcttgcga caggtcgcca
     3601 gcctcgatgg cgtcgtacgc ggctgccatg gtcgacctct cagcggaggt cggcacgtcg
     3661 tagtcttcac tgtatgctct
//
LOCUS       DATAIR010000229         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_291637, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000229 DATAIR010000000
VERSION     DATAIR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291637"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..389
                     /locus_tag="VHM69_05465"
     CDS             213..389
                     /locus_tag="VHM69_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739875.1"
                     /translation="MPRDPQSRTADDSAQPAGRPSGLAPEEVEEPGRTQEEADWLSMA
                     TADPALFLKDSGSE"
     gene            complement(418..684)
                     /locus_tag="VHM69_05470"
     CDS             complement(418..684)
                     /locus_tag="VHM69_05470"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX2739876.1"
                     /translation="MDEHRLDGNAAAGVLAEIFTFEMTTAHCACANCGATGAVGAQMA
                     YVSEIGTVVRCAACDNALIRVASGSGRYWLDLRGVEYVQLEDRL"
     gene            complement(687..1427)
                     /locus_tag="VHM69_05475"
     CDS             complement(687..1427)
                     /locus_tag="VHM69_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX2739877.1"
                     /translation="MAPRMLTWRVGEVVETIPDTQRTKRLFLEVPGWEGHRAGQHVDV
                     RLTAEDGYQAQRSYSIASAPDDGLVELLVERLEDGEVSPYLTDELRIGDGLELRGPIG
                     GWFAWEAREGGPLLLIAGGSGIAPLMAMIRHHKAAGSDVPVRLLFSSRSYEDVFFREE
                     LESLAAENNTLEITHTLTRSTPPGWTGYDRRIDEEMLGEVAFSPEDRPLAFVCGPTPL
                     VESVATDLVGLGHDPTRVKTERFGPTGG"
     gene            complement(1429..2040)
                     /locus_tag="VHM69_05480"
     CDS             complement(1429..2040)
                     /locus_tag="VHM69_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX2739878.1"
                     /translation="MGRGFFSRGFRGRRRQVEGDVRIPPGQYLVDGFPVLSAGPTPRT
                     RLSEWTFSIAGEVDEPKTWSWEEFRALPSEEISRDIHCVTKWSKLGTTWEGVSVDTLL
                     EGVDTAAEYVVAFCDGGYTTNLPLEDVTGGKAWIAHTYEGEPLAPEHGGPARLLVPHL
                     YFWKSAKWVRGLKLAAEDEPGFWESLGYHNYGDPWREQRYWGD"
     gene            2228..2458
                     /locus_tag="VHM69_05485"
     CDS             2228..2458
                     /locus_tag="VHM69_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739879.1"
                     /translation="MTAERYTAVFTGLLSLKKPGEYLYVRMSEDPFGSGSYTINRGRP
                     PYGELGRGVTFGDLPEGCKSLVLETYRKVWNL"
     gene            2952..>3953
                     /locus_tag="VHM69_05490"
     CDS             2952..>3953
                     /locus_tag="VHM69_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739880.1"
                     /translation="MTVCDELAQAYSTIAKVDAFAYASMNTSIGKLSPDPDWLGPVRN
                     ELKLLSNAGAGWQQKKPDIWAPLLSQFNTYHALFSSYAEMASQMGDNKDAWLEALGQL
                     RTSLANAEIASKAAEGQFVLQINNLNNVRQVFDSSLNQAWKSLADEEQEMIALASEVT
                     ALQDKVNSLQEKINSTEISSGQSYIQSSVSISYTLVTTAGAEVPYLAIVGLVFTVGKL
                     AYDLIVTDEEITETIGKIVELRNKASQEAQAAAMSKGIIQLIDNFDKSLLAVQRRLPA
                     LGTMWANEKAKVDGAITALNSGAQPTQYLELLSMASAAATWKKLADFATQITQKSEQG
                     "
BASE COUNT          811 a         1202 c         1205 g          735 t
ORIGIN      
        1 cgccgaggag catggctaca ccgcgaccaa gcaccagcgc gaggtcggcg cgggatactt
       61 cgacgaggtc gcccagatcg tggccggcgg cagcgcctcc acgacggccc tcacaggctc
      121 caccgaggag gctcagttca ctaaataggc gtcaggctac aggcatcagg gggagccggg
      181 tcttccggct ccctccgaaa aaggaggaga caatgccgag agacccgcag agccgtacgg
      241 cggacgatag cgcgcagccg gctggaagac cctctggtct tgctccggaa gaggtggaag
      301 agccgggacg gacgcaggag gaagcggatt ggctatccat ggccacggcg gacccggcac
      361 tgttcctcaa agactcgggt tctgagtaga gagcctttcc cgagatgctg ccagagttta
      421 cagtcgatct tccagctgca cgtactctac gcccctgaga tccaaccagt aacgtcctga
      481 gccgctcgcg acgcggatga gggcgttatc gcacgcggcg cagcgcacca cggtacctat
      541 ctcgctcacg taagccatct gggccccgac cgcgccggtc gcgccgcaat tggcgcaggc
      601 gcagtgggct gtggtcatct cgaaagtgaa gatctctgcc agtacgcctg ctgctgcgtt
      661 gccgtcgagc ctgtgttcgt ccatcactat cctccagtgg gtccgaagcg ttccgtcttg
      721 actcgcgtgg ggtcgtggcc gagcccgacg agatccgtcg cgaccgactc gaccagcggg
      781 gtaggaccgc atacgaaggc gagggggcgg tcttcgggcg agaaggcgac ctctccgagc
      841 atctcttcgt cgatcctgcg gtcgtacccc gtccagcccg gtggcgtcga gcgggtcagg
      901 gtgtgggtta tctccagagt gttgttctcc gcggcgaggc tttccagctc ttcgcggaag
      961 aagacatcct cgtaggagcg cgaggagaag agcaggcgga ccggaacatc gctgcccgcc
     1021 gccttgtggt gccggatcat cgccatcagc ggcgcgatac ctgatccacc tgcgatgagg
     1081 agcaaagggc ctccttccct ggcttcccag gcgaaccagc ccccgatcgg accccgcaac
     1141 tccagcccgt ccccgatcct gagctcgtct gttagatagg gggagacctc gccgtcctcc
     1201 agacgctcga cgaggagttc caccagtcca tcgtccggcg ccgaggctat ggagtagctg
     1261 cgctgcgcct ggtagccgtc ctcggcggtg agcctgacgt caacgtgctg gcccgccctg
     1321 tgcccctccc agccagggac ctccaggaac aggcgcttcg ttcgctgtgt atcggggatg
     1381 gtctcgacga cctcgccgac gcgccaggtg agcatgcgcg gggccatctc agtctcccca
     1441 gtagcgctgc tcgcgccacg ggtcgccgta gttgtggtag cccagggact cccagaatcc
     1501 aggctcgtcc tctgccgcga gcttgaggcc gcgtacccat ttggcgctct tccagaagta
     1561 gagatgtggg acgagcaggc gggcaggacc gccgtgctca ggcgcgagcg gctccccctc
     1621 ataggtgtga gcgatccagg ctttccctcc agtgacgtct tccagcggaa ggttcgtcgt
     1681 atagcctccg tcgcagaaag cgaccacgta ctcggccgcc gtatctactc cttccaacag
     1741 agtatccacg gagacgcctt cccaggtcgt tccgagtttc gaccacttgg tgacgcagtg
     1801 gatgtccctg gagatctcct cagacggcaa cgcgcggaac tcttcccacg accacgtctt
     1861 tggctcgtcg acctcgccag cgatcgaaaa agtccactcc gagagccgcg tgcgtggtgt
     1921 ggggcctgcg gagaggacgg ggaagccatc cacgaggtac tgtcctggcg ggatcctgac
     1981 gtcgccctcc acctgcctgc ggcggccccg aaagccgcgg gagaagaatc ctctgcccat
     2041 gccgataccc cctgctgact cgctatgccg ggaaagtgtt cattgtggca tgatgcgtct
     2101 ggtggggtca acgtgcgtac ccggcagatt tcctcacgag ccttacaatg tggccatgaa
     2161 ggaaaggcga gagagactct tacccaacgg agtaccgcac tacgtctgac tctacgacga
     2221 cggcggcgtg accgccgaac gttatacggc ggtcttcact ggtcttctct cgctcaagaa
     2281 gccgggcgag tacctctacg tgcgcatgag cgaggaccct ttcggttccg ggagctatac
     2341 gatcaaccgt ggccgtccgc cttacgggga gttggggcgc ggggtcacgt tcggggatct
     2401 tccggagggt tgcaagagcc tcgtactgga gacgtaccgg aaggtgtgga acctgtagcg
     2461 ataacgaatc cctgcgcacg gagcaaacct attcaggcga gtagaatctg cctgtcttgc
     2521 ccattgtttt cgcctactta cacgtaacat gatgcctgag ctctgatcgg ttcgcggaag
     2581 ccggccaatt caccatcact tgaatcaaca gaccactgag aattggcgga attcattgcg
     2641 aattcatccc ggattcaccc cggactcatt gctctttgcg tcaatgccag gcgcatgggc
     2701 cggacaaccc gaggaaagga gtgagctatg gggcgtgatc gcgattagaa gcgcggcgga
     2761 tcagggctta ggtcaacacc actggtgagg ggccggaaaa ccgattcgtc ggtgaccggt
     2821 catgccagct ctcccacaac acgaggcgga tcgctttgct gagatcgttg tgaaaaggag
     2881 aggatttcca tgagtaacag gcgcgttgct gaattagaag ggaggtaagg gccatgctat
     2941 gcccaaacgt catgaccgtc tgcgacgagc tagcgcaggc ctattcgacc atcgccaagg
     3001 tggatgcgtt cgcttacgct agtatgaata cttccatcgg gaagctttcg ccagatccgg
     3061 attggctcgg cccagttcgc aatgaactca agttgttgag caacgccggg gcaggatggc
     3121 agcagaaaaa gccggacatc tgggcgccgc ttctgtccca gttcaatacc taccatgcgc
     3181 tgttctcgag ttatgccgag atggcgagcc agatggggga taacaaggat gcctggctgg
     3241 aagctctggg ccagctaaga acttcgctgg ccaatgcgga aatcgccagc aaggccgccg
     3301 aggggcaatt cgttctacag atcaacaacc tgaacaacgt caggcaagtc tttgattcca
     3361 gcctgaacca ggcctggaag tctctcgccg atgaggaaca ggagatgatc gcactggcgt
     3421 ccgaggtgac cgcgcttcag gacaaggtca actccctcca ggagaaaatc aacagcacgg
     3481 agatatccag cggacagagc tatatccaat catccgtcag tatcagctac accctcgtca
     3541 ctacagctgg ggccgaagtg ccctatcttg cgatcgtcgg tctcgtcttt accgtaggca
     3601 agcttgccta cgacctgatc gtcaccgacg aagagatcac cgagacgatc ggcaagatcg
     3661 tcgagcttcg gaacaaagct tcacaagaag cacaggccgc ggcgatgagc aaggggatca
     3721 ttcaactcat agataacttt gacaagagct tgctcgccgt tcaacgtagg cttcctgctc
     3781 tcggcacgat gtgggcgaac gaaaaggcga aggtcgatgg ggctattacc gcgctcaact
     3841 ccggagccca accgacccag tatctcgagc tgctctccat ggcgtcggcg gccgcaacct
     3901 ggaagaagtt ggcggacttc gcaacgcaaa ttacccagaa gtccgagcag ggc
//
LOCUS       DATAIR010000230         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_329477, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000230 DATAIR010000000
VERSION     DATAIR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329477"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VHM69_05495"
     CDS             complement(<1..1068)
                     /locus_tag="VHM69_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX2739881.1"
                     /translation="MRESRPRNLKEMLVEAKNTSELMVDLSYTAIFYNSEDLSEEVFR
                     LEERLNDLVFDMRTLAILAARSPSDAEQMAGILQVVQDIEKIGNAAYDIAKIVVKRLG
                     IPPELLHDIPEAEEIPSRVRVHAESALDGRPLGDVDLPVETGMRPIALRSEDEWNFDP
                     EPEDVLRAGDVLFLQGPPEGVDEVRELAGARPLGDAPESTPPPGELSEIDRAVDILIE
                     MKNFSEVAVGLAYSALLYDDAGLAREVVAIEDEMDEMRYRLERWVLLAVPHVEDPSRL
                     RGLLHLATAAETIANCAMEMVWVVEKGEEVHPVLSAAVEESDEVVLKLTVSPGSPADG
                     KTLGSLRIETETGMYVLAVNRG"
     gene            complement(1073..2437)
                     /locus_tag="VHM69_05500"
     CDS             complement(1073..2437)
                     /locus_tag="VHM69_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX2739882.1"
                     /translation="MLRADTYTALYIVCGNGYTSTVAEAETKGSKSRSGEGGIRQRLR
                     DRPRLRPKVYGYLTEEKESLWQGFAALFLSSAGELIAGIVLAGIAGILDELVGLAVLI
                     PAAIGMRGAIFGAMGSRLSTSIQTGLFSFNLRRGVLAENVQAAAVLSLVSGVFLAVLA
                     RILCGILGVRTELSVVDYIVISTVGGIIAGVALLVITVLVARLSVARGWDMDNIASPM
                     LTAAGDILTLPALVLATHLIGIPVFSWILGVALVLAAGAAAYYGFRPRVENLRRILAE
                     SFPILALTGAVTILVGVALQNREEQFTTLLALSILLPAFLQEGGALGGILSSRLSSKI
                     HLGLIAPRGIPQLAAFRDFTLTYIFAAGVYVFIGGASHVIAVALFPEGASPGFLAMIG
                     VSALAGLIATTAAVLAAYYGSTVSYRLGLDPDTCGIPIITAAVDLLGFMSLIIAMIAF
                     GLTG"
BASE COUNT          455 a          779 c          825 g          407 t
ORIGIN      
        1 gcccctgttc accgcgagga cgtacatccc tgtctcggtc tcgatcctta aagacccgag
       61 cgtcttgccg tcggcggggg agcccgggga gacggtgagc ttcaggacga cctcgtcaga
      121 ctcttcgacg gctgcggaga ggacaggatg gacctcctcc cctttctcga cgacccagac
      181 catctccatc gcgcagttgg cgatggtctc cgcggcggtg gcgaggtgca gcaggcccct
      241 caggcgagag gggtcctcca cgtgggggac ggcgagaagg acccagcgtt cgaggcggta
      301 acgcatctcg tccatctcgt cctcgatggc gacaacctcg cgggcgagac ccgcgtcgtc
      361 gtagagcagg gccgagtacg cgagcccgac ggcgacctcc gagaagttct tcatctcgat
      421 caggatgtcc acagcgcggt cgatctccga gagctcgcca gggggcggcg ttgactcggg
      481 agcgtccccg agcgggcggg cgccggccag ctccctgacc tcgtcgacgc cctcgggcgg
      541 gccttgaagg aagaggacgt cgccggcccg cagcacgtcc tcgggctcgg ggtcgaagtt
      601 ccactcgtcc tcggatcgga gggcgatggg gcgcatcccc gtctccacgg ggaggtccac
      661 gtcgccaaga gggcggccgt caagggcgct ctcagcgtgc acgcggacgc gggacggtat
      721 ctcctcggcc tcggggatgt cgtgcaggag ctccggcggt atcccgagtc gcttcacgac
      781 gatcttggcg atgtcatatg cggcgttgcc tatcttctct atgtcctgga cgacctggag
      841 tatgcccgcc atctgctcgg cgtccgacgg gctcctcgct gccagtatgg cgagggtacg
      901 catgtcgaag accaggtcgt tgaggcgttc ctccaggcgg aacacctcct ccgagaggtc
      961 ctctgagttg tagaagatgg ccgtgtagga gaggtctacc ataagctccg aggtgttctt
     1021 cgcctcgacg agcatctcct tgaggttccg cggtcgcgac tctcgcatgt ccttaccccg
     1081 tcagtccgaa agcgatcatg gctattataa ggctcataaa gccgagcagg tccaccgcgg
     1141 cggtgatgat cgggatgccg cacgtgtccg ggtcgagccc gagcctgtag cttaccgtcg
     1201 agccgtagta ggctgcgagg actgccgccg tcgtcgctat gagcccggca agagcgctta
     1261 ccccgatcat cgcgaggaaa ccggggctcg cgccttcagg gaaaagcgcc acggctataa
     1321 cgtgcgacgc tcccccgata aacacataca ccccagcggc gaagatgtat gtgagcgtga
     1381 agtccctgaa cgccgcgagc tgcgggatcc cacgcggcgc tatgagcccg aggtgtatct
     1441 tcgaagagag gcggctggag aggatcccac ccagcgcgcc gccctcctgg aggaacgcgg
     1501 gcaggaggat gctcagggcg agcagtgtcg tgaactgctc ttcgcggttt tgcagggcga
     1561 ctccgaccag gatggtcacg gcccctgtga gggcaagtat ggggaacgac tcggccagga
     1621 tgcgcctgag gttctcgaca cgaggcctga agccgtagta tgcagcggcc cccgccgcga
     1681 ggacaagcgc gactcccagg atccaagaga agaccgggat gccgatcagg tgggtcgcga
     1741 ggaccagtgc ggggagcgtc aatatgtcgc cggccgcggt cagcatcggc gaggcgatgt
     1801 tgtccatgtc ccacccgcgg gcgaccgaga ggcgggctac gaggaccgtg atcacgagca
     1861 gcgcgacgcc ggcgatgatc ccacccaccg tcgaaatgac tatgtagtcg acgacggaga
     1921 gctccgtcct tacgccgagt atgccgcaca gaatgcgcgc caggacagcg aggaaaacgc
     1981 ccgagacgag cgagaggacg gcagcggcct gaacgttctc ggcgagaacg ccgcgtcgca
     2041 ggttgaaact gaagaggccg gtctggatgg aggtcgagag cctgcttccc atcgccccga
     2101 agatggcgcc gcgcatccct atggccgcag ggatgaggac ggcgagcccg acgagctcgt
     2161 cgaggatgcc ggcgatgcca gcgagcacga tccccgcgat gagctcccca gcagaggaga
     2221 ggaagagcgc agcgaacccc tgccagagcg actctttctc ctccgtgagg tagccgtaga
     2281 ccttggggcg caggcgcggc ctgtcgcgca gcctctggcg tatcccgccc tctccgcttc
     2341 gggacttgga tccctttgtc tctgcctcgg ccacggtaga ggtatagccg ttgccacaga
     2401 ctatgtaaag cgctgtgtaa gtatcagctc tcagcacgcg cagatccaca aagcatctac
     2461 atcatc
//
LOCUS       DATAIR010000231         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_429773, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000231 DATAIR010000000
VERSION     DATAIR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429773"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VHM69_05505"
     CDS             <1..781
                     /locus_tag="VHM69_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739883.1"
                     /translation="TGYPNISVYHDFTYEPKKYITGAFDDWAYDYYGVFAFTIEFWSM
                     ARAAGVEVEDFIEFFRNPPEEASLKMLAWNDRELGGEGFVPWSPFDHPQLGRVEIGGW
                     KTKFTFQNPPPKYLEAECEKLARFALSHASTAPRLRTRLATEELSPGLHRLELIVENA
                     GYLPTNVSRVATDKKLARPVEVRIELPQGASLVSGEPEVELGHLAGRSALTGNRWKSP
                     AFFEGLSSDYASRTVWVVRGDGPVEVEVRGGRAGTARLRTE"
     gene            complement(841..1122)
                     /locus_tag="VHM69_05510"
     CDS             complement(841..1122)
                     /locus_tag="VHM69_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739884.1"
                     /translation="MYYPKRRSAIREGRYRTPDRRQQIRMREVLKDRFESIPVQVLRS
                     DIEALGVEIGLDDPAEAARIFDRLKGISWRGDYVRSDEGWVAAWVREVD"
     gene            complement(1274..>4391)
                     /locus_tag="VHM69_05515"
     CDS             complement(1274..>4391)
                     /locus_tag="VHM69_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2739885.1"
                     /translation="ELPSQAGEPEVKSQSASQFPILTLSLAARDKDLSGLTEYAEDEV
                     VPSIEEVDGVASVDLIGGAEKRVEVDLDPEKLKEKGISTDAVVGALSGAKVDAPVGSV
                     SVEGRETPVSATSEIGDTEALEDLPVGVSGGATGGAPPGVIPGAASAPPAGASVAPSS
                     IPTASASPAAGAPEPVLLGDVAEVREVGSALSGISRTNGEPSLGLNVVKETDANTVEV
                     AADVEKVLDDVRKRIGEDQVVVVANSATDVEESVNGLVEEGLVGAVLAILVIFAFLRS
                     IRATLVTAVSLPTSVLAALLFSWADNLTLNIITLAGLTIAVGRVVDDAIVVLENSYRY
                     VQGGYEPEEAALKGTTEVASAITSSTLTTTAVFVPLALVGGIVSKFFVPLSLTVALAL
                     LASLVVAVTIIPVLVSLFLKRRTAVDASQRTDEDFEEVPEYESRRMRRAGTRNGGGFA
                     RFVAGVLMLALASALALAVASRAGLLDDVPGLPSGFVDAVDGFVSEIGPGSPIFLVVV
                     AVIGALLLVGLLLLVVRMARRSRGESEGLLVRLYTPMLRWSLRHRLAVLLFALLAFVG
                     GLGLVQLLPVSFFPPSEERLLVADVELAAGTGLDQTSQKLRPFEDFLEGDESIKSYQV
                     SIGGEDTLDPESPVRPGNKAQAFINVKESADVRRALDRVDHKGDELYGDNFQVQIISN
                     GPPQGGLEAVLTGGSKQDLAKAADLVAREFRKLDDVNNVQSDLSGGNPEVEVKVDPER
                     AAKAGLSPGVVSSSLGSLLGDSTVTTLGDTPVVVGVPESSVDSLDEVRELPVGSGITV
                     GDVAEVREVESPAAVSRTDGERAVTVTGEITSTDTQSVSTEAQEAMADLDLPGNVKAQ
                     VGGENEDIDESFRNLFLSIIVALVLVFLILVVFFGSLLVPMVILLAVPLTTVGAFGAL
                     YLTNTAISVPSLLGILLLIGIVVSNAILLVDFAIKAHDRYETSDEAILAAGQARLRPI
                     LMTAFATVFALLPLALGLSGGGNGLISSSLAITVVGGLATSTFLTLLVVPVGYSLLKS
                     GRRRRKKI"
BASE COUNT          815 a         1467 c         1360 g          749 t
ORIGIN      
        1 cacaggctac ccgaacatct ccgtctacca cgatttcacc tacgagccga agaagtacat
       61 aaccggcgcc ttcgacgact gggcctacga ctactacggc gtcttcgcct tcaccatcga
      121 gttctggtcc atggctcggg ccgcaggcgt cgaggtcgaa gacttcatcg agttcttccg
      181 aaacccgccg gaggaggctt cgctgaagat gctcgcctgg aacgacaggg agctgggcgg
      241 cgagggcttc gtcccgtgga gtcccttcga ccacccccag ctagggcgcg tggagattgg
      301 cggctggaag accaagttca cattccagaa tccgcctccg aagtacctcg aagcggagtg
      361 cgagaagctg gcccgcttcg ccctctccca cgcctccacg gccccacgcc ttcggacccg
      421 ccttgcgacc gaggagctct cacccggact ccaccgtctg gagctgatcg tcgagaacgc
      481 gggatacctg ccgacgaacg tcagcagggt agccacggac aagaagctcg ccaggcccgt
      541 cgaagtcagg atcgagttgc cgcagggcgc gagcctcgtg tcgggagagc cggaggtcga
      601 gctgggacac ctcgcggggc gctccgcgct caccggcaac aggtggaaga gcccggcctt
      661 cttcgagggt ctgtcctccg attacgccag caggacggtc tgggtcgtgc ggggcgacgg
      721 tccagtcgaa gtcgaagtgc gcggcgggag ggccggcacc gcgcggctca ggacagaata
      781 ggagacctct cgcatcgtga catcagcttt cagtctttta ttcttgctga ctgaagtgct
      841 ctaatccact tccctgaccc aggctgcgac ccagccttcg tcggacctga cgtagtcacc
      901 cctccacgaa atgcctttca gccggtcgaa gatcctggcc gcttcggccg ggtcgtcgag
      961 cccgatctcg actcccaaag cctcgatgtc actccgcaga acctggaccg ggatgctctc
     1021 gaacctgtcc ttcagcacct cgcgcatcct gatctgctgc cgcctgtcgg gggtccggta
     1081 cctgccctct ctgatggccg acctcctctt cgggtagtac aacgacagtc tgcctccccc
     1141 ctcgaaacaa aactgtcgta caaaacacta cgcaaggtct attgtcggcc gatcatctcc
     1201 aatactgaat ctacaaaccg cgctgaaacg ttggcgtccc cggtttcgag tctcttctgc
     1261 gagcgccgga cttctagatc ttcttcctcc gcctcctgcc gctcttgagc agcgagtagc
     1321 cgacgggaac gacgagcagc gtcaggaacg tcgaagtcgc aagacccccg acgaccgtta
     1381 ttgcgaggct cgacgagatg agcccgttgc cgccgccaga cagcccgagc gcgagcggca
     1441 gcagggcgaa gacggtggcg aaggccgtca tcaggatggg cctaagacgc gcctggccgg
     1501 ccgccaggat cgcctcgtcc gaggtctcgt agcggtcgtg ggccttgatc gcgaagtcaa
     1561 cgagcaggat agcgttcgag accacgatcc cgatgaggag caggatgccg agcaaggaag
     1621 gcacggagat ggccgtgttc gtcaaatata gagcgccgaa ggccccgacc gtcgtgagag
     1681 gtacggcgag aagtatcacc atcgggacga ggagcgagcc gaagaagact accaggatca
     1741 ggaacacgag caccagcgcc acgatgatgg agaggaagag gttcctgaag ctctcgtcga
     1801 tgtcctcgtt ctctccacca acctgcgcct taacgttgcc aggcaggtcg aggtcggcca
     1861 tggcctcctg ggcctccgtc gagacgctct gggtgtcggt cgaggtgatc tcaccggtaa
     1921 ccgtgacggc acgctcgccg tcggtgcggc tgacggccgc gggcgactcc acctccctga
     1981 cctcggccac gtccccgacg gtgatcccgc tcccaacagg caactcacgc acctcatcca
     2041 gcgaatctac ggaactctct ggcacgccaa cgacgaccgg cgtatccccg agagttgtca
     2101 ccgtgctgtc acccagcagg gagccgagcg aactcgagac cacgccgggc gagagtcctg
     2161 ccttcgccgc tctctcgggg tctaccttga cttcaacttc agggttacca ccggagaggt
     2221 cactctgtac gttgttcacg tcgtcgagct tgcggaactc tcttgcgacg aggtcggcgg
     2281 ctttggcgag atcctgcttc gatccgcccg tgaggacggc ttcgagtccg ccctgcggcg
     2341 ggccgttgga gatgatctgg acctggaagt tgtccccgta gagctcgtcg cctttgtggt
     2401 ccacccgatc cagcgctcgc ctcacgtcgg cgctctcttt tacgttgatg aacgcctgtg
     2461 ctttgttccc cggcctgacg ggggactcgg ggtcgagggt gtcctccccg ccgatggaga
     2521 cctggtagct ctttatgctc tcgtctcctt ccaggaagtc ctcgaacggt cgcagcttct
     2581 ggctcgtctg atccaacccc gtcccagcgg ccaactcgac gtccgcgacg aggagccgct
     2641 cctcgctcgg cgggaagaag ctgacgggca aaagctgaac gagcccgagc cccccgacga
     2701 aagccaggag cgcaaacagc aacacggcca gcctatgcct gaggctccag cgcaacatcg
     2761 gggtgtagag ccggacgagc agcccctcag actcaccacg agaacgccgc gccatccgca
     2821 cgacgagcag caggagcccc acgaggagca gtgctccgat cacagcgacg accacgagga
     2881 agatgggtga accgggacct atctccgaga cgaacccgtc aacggcatca acgaagccgg
     2941 aaggaagtcc gggcacgtcg tcgagtagac cggccctcga ggcaacggcg agtgcgagcg
     3001 cggaggcaag cgcgagcatc aggacgccgg ccacgaagcg ggcgaaaccg ccgccgttcc
     3061 tcgtcccggc ccgccgcatc cgcctgctct cgtactccgg cacctcttcg aagtcttcgt
     3121 cggtcctctg tgacgcatct actgcggtcc tgcgcttgag gaagaggctg acgagcacgg
     3181 ggatgatggt gacggcgacg acgagggagg cgagcagcgc gagggcgacg gtgagcgaga
     3241 gcgggacgaa gaacttggat acgatgcctc cgacgagggc gagtgggacg aagaccgccg
     3301 tagtcgtgag cgtcgacgag gtgatcgcgc tggcgacctc tgtggtcccc ttcagcgcag
     3361 cctcctcggg ctcgtaaccg ccctgaacgt agcggtacga gttctcgagc acaacgatcg
     3421 cgtcgtcgac caccctgcct accgctatcg tcagtccagc gagggtgatg atgttcagag
     3481 tcaggttgtc ggcccacgag aagaggagtg cagcgagtac agaagtaggc aaagagacgg
     3541 ccgtgaccag ggtcgcgcgt atcgagcgca ggaaggcgaa gatcaccagt atcgccagta
     3601 cagccccgac gagcccctcc tcgacgagcc cgttcaccga ctcctcgacg tccgtggccg
     3661 agttggcgac gactacgacc tgatcctccc caatcctctt ccgcacatcg tccaacactt
     3721 tctctacgtc ggcggcgacc tctacggtgt tggcgtcggt ctccttgacg acgttgaggc
     3781 cgaggctcgg ctccccgttg gtgcgtgaga tccccgaaag ggccgacccc acctcacgca
     3841 cctcagccac gtccccgagc agcaccggct ccggagcccc ggccgcaggc gaagcgctcg
     3901 ctgtagggat gctggaaggc gcgacggatg ctccggccgg tggagcgctt gccgcgcctg
     3961 ggataactcc cggcggggct ccgccggtcg cgccgcctga aactccgacg ggcaggtctt
     4021 cgagggcttc ggtgtcgccg atctcgctgg tggcgctgac gggggtttcg cgcccttcca
     4081 cggagacgct acctacgggc gcgtcaactt tcgccccgct cagggcgccg accacggcgt
     4141 cggtcgagat gcccttctcc ttcagcttct ccgggtcgag atccacctcg acacgcttct
     4201 ccgcgccccc gatcagatcc acgctcgcaa cgccgtcgac ctcctcgatg gagggaacga
     4261 cttcgtcctc ggcgtactcg gtgagcccgg aaaggtcctt gtccctggcg gccagggaga
     4321 gcgtgaggat gggaaactgc gacgccgatt ggctcttgac ttcgggctct ccggcctgcg
     4381 acggtaactc t
//
LOCUS       DATAIR010000232        10102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_383677, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000232 DATAIR010000000
VERSION     DATAIR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383677"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1447)
                     /locus_tag="VHM69_05520"
     CDS             complement(173..1447)
                     /locus_tag="VHM69_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX2739886.1"
                     /translation="MASQAVSSSGAGNRVIIAIAGVVMQVALGAVYAWSVFADPLAQA
                     FGGASATAVNSTFSITIFTLGFAAFVGGLWMGNVGPRTVALTAGVLYGLGIFLAGFAS
                     NSLLLLYLTYGFIAGAGIGLGYIVPVATLIKWFPDKRGFITGIAVAGFGGGAFVTALV
                     AKSLVSSVGPFRTFTILGIIYLVMVVGAALFMKNPPEGWKPEGWEPETTESGDSSEVN
                     YDLGGALKTWQWFALWALLFLNVTAGISIITVASPMAVTLAGVSATVAAGLVSIISVG
                     NALGRFFWAWLSDAIGRKWVFFVMFLLQAVLFILLPFLGTVYVLLAIISFAIISCYGG
                     GFGTMPAFAADYFGSANVGKIYGLMLTAWSFGGVLGPLLISRVIDVTSSYTLAFYIIA
                     AIMVGSAVVAFIVNPPKQKSEASATQTEGAKA"
     gene            complement(1574..2437)
                     /gene="fdhD"
                     /locus_tag="VHM69_05525"
     CDS             complement(1574..2437)
                     /gene="fdhD"
                     /locus_tag="VHM69_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX2739887.1"
                     /translation="MLEKTSGKPRRGSKTKTRIRVVEEGRMRMRPDTLATEEPMEIRL
                     LAGDTKQTVAVTMRTPGADFELAAGFLYGEGIVSSSDDILKISYCVDSDLDAEQRYNI
                     VNVELRGNREYDLRPLERHFYTTSACGVCGKASLEQLELRGCPIIPPGPTLTARTIYG
                     MPEKLREAQGLFDVTGGLHAAALFDNNGELIALREDVGRHNATDKLVGWALLEGRLPI
                     SDHVVMVSGRSSFEILQKCLTAGVPIVCAISAPSSLAVDVAKEFGMTLVGFLREGRFN
                     VYAGSDRILPE"
     gene            2526..2747
                     /locus_tag="VHM69_05530"
     CDS             2526..2747
                     /locus_tag="VHM69_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739888.1"
                     /translation="MGLPNVARYPEATVVRDETHILITFGGLHGEHTMGVPLEYVGGD
                     EEIAELRLLAQLQHIGYRVRRGERDPSDP"
     gene            complement(2773..3414)
                     /locus_tag="VHM69_05535"
     CDS             complement(2773..3414)
                     /locus_tag="VHM69_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX2739889.1"
                     /translation="METVKTPAEQRVVLHNIGWNTYERLLADHENNSAPRFTYDRGEL
                     EIMSPSPEHEAFNRSIALIVEFLAAELGIDVYDLGSTTFRREDLERGFEPDSCFYIQN
                     EEQVRGKDRIDLAVDPPPDLVIEIDITSPSFSKLPIYAQIGVCEVWRYDGKRLTILVL
                     EGSDYAESTDSTVLPPATNTALTGFVEKSKATRRTIWLKTIREWARKQIEPAP"
     gene            complement(3522..4730)
                     /gene="fdhA"
                     /locus_tag="VHM69_05540"
     CDS             complement(3522..4730)
                     /gene="fdhA"
                     /locus_tag="VHM69_05540"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEX2739890.1"
                     /translation="MADNRVVVYKGPGEVAVEDIEYPKLEVPGEVADAMGMSKEAPHA
                     AILKIVSTNICGSDQHMVRGRTTAPVGQTLGHEITGEVVEKGNDVQFIDVGDICSVPF
                     NITCGRCRNCNEGKTGVCLNVNPARAGSAYGYVDMGGWTGGQAEYVMVPFADFNLLKF
                     PDRDQALEKILDLTMLSDIFPTGYHGAYTAGTTTGSTVYVAGAGPVGLAAAYSAQLLG
                     AAVVIVGDLNDERLAQARSFGCETVNVGEGAELPDQISEILGVPEVDCAVDAVGFEAS
                     ARPGEESPAVVLNQVQTITRAGGALGIPGLYVTGDPGAADPDAQEGTLKIRFGLGWAK
                     SHAFYTGQCPVKRYNRGLMMSILHDKAHIADAVNATVITLDEAPQGYKDFDKGAAKKY
                     VLNPNGYIDA"
     gene            5042..5608
                     /locus_tag="VHM69_05545"
     CDS             5042..5608
                     /locus_tag="VHM69_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739891.1"
                     /translation="MLFKVTEKDGLWNKIRNHVGTTVKHPISPATRDIRSRISDDGVE
                     KTVSVCPYCAVGCSTLVYHRDGRIIDIEGNPDSPVNGGTLCPKGSATFGMHVSPHRKT
                     KVMYRRPYSDQWEHLTLDQALDMIADRVKDAREETWEEKDEQGKKLNRSMAVASIGGA
                     TIDNEENYLITKLFHSLGFVRITNQARI"
     gene            5621..8149
                     /locus_tag="VHM69_05550"
     CDS             5621..8149
                     /locus_tag="VHM69_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2739892.1"
                     /translation="MPGLGITYGRGGATSNLQDLQNADCILIEGSNMAEAHPVGFRHP
                     MIAKKKGAKLIHVDPRFTRTSAMCDIYAPLRPGTDIVFLGGLINYVLENEEYFEEYVK
                     IYTNAATIITDDFEDADASGLFSGWDEESKQYDPSTWRYEGHPIDYASVGGSQGRIVG
                     EAGEGEHGEMHPGMPRPTDMSLQHPRCVFQILKRHFAKYTPEMVEKVCGTPKEAFLEV
                     AKTLAENSGREKTSSLCYAVGWTQHSKGVQVIRTAAILWLLLGNIGRPGGGIMALRGH
                     ATIQGSTDIATLYNILPGYLPMPDTNKDHDTWQDYVENETASSGWWSNFPKYITSQMK
                     AWYGEKFDAESGALFDQFPRLSGDHSYYPTIMDMKDGKVRGAFIFGQNLAVGGPHAKL
                     ARDGLRNLDFLVVLDAYEIETSTVWKLDGVKPEECGTEVFFMPMALVAEKDGSFTQTQ
                     RMLQWHDKSVDPPGEARSDAWFVFHLGRRMKELYKGSRKHRDRLIRAMTWDYPTEGER
                     EEPDIESVLKEVSGYKVETGEPVGGFAELKDDGSTACGGWIYSGVYAGGENKARNRKP
                     GGNPNLEWGWAWPMNRRILYNRASADPEGKPWSERKKYVWWDEEENKWVGDDVPDFQA
                     DKPPSYRPDEDAGGVDAIPGDGPFIMNGDGKGWLFAPVGLKDGPLPVHYEAVESPVPN
                     LLYEKVQHDPVAKTFDRPDNPYNPPEDPRFPYVVTTYRLTEHHTSGAMSRWIPWLNEL
                     QPELFCEISPTLAAEKGVKNTDWVTITTSRGKVHCKALVSDRIPVYKFDGKDLHTIGL
                     PYHWGPNGLVKGDVVNDLIPVSLEPNVAIHEAKAFTANLEAGRV"
     gene            8154..8621
                     /locus_tag="VHM69_05555"
     CDS             8154..8621
                     /locus_tag="VHM69_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739893.1"
                     /translation="MGTKQRALAGAVSGLGGTLVLSGLREAWARIGLVFETAPMQVVA
                     RVEELGLVGDLSPAGRRLLTVIAHFAYGAGTGTAFGLLRRETGGPEEEASVGSALGIL
                     AWGAGWASWLPLTGVHEPPWTQRTPRVLLPVIDHAVFGAAWGLLYWRLIRGRV"
     gene            8618..8902
                     /locus_tag="VHM69_05560"
     CDS             8618..8902
                     /locus_tag="VHM69_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739894.1"
                     /translation="MSVEEIRSREMMEQELMDLGVWLIEHPDVVEKNLKPIRSAKILE
                     PGVYYNMGTGMVDRIYSPQRVALGHKMFRITRDPASPVEEIRRIALEGKG"
     gene            8902..9738
                     /locus_tag="VHM69_05565"
     CDS             8902..9738
                     /locus_tag="VHM69_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2739895.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELTGDSYDNTK
                     QLSGTTWRHVAFIENPKKRRLTGDVDLVALAEDVTEQLPGGAELDALGRWAFMSDVCK
                     HCVEAPCQQACPTGSIIRNEFDNVYIQPDICNGCGYCVPACPYGVITQVPHDGRAFKC
                     TLCYDRQKDGLEPACAKACPTNSIMFGEVEELKQIAEERVQELHDKGYSQAYVWGTPE
                     VGGTGGIKGNHSKFILMDEPEVYNLPRHPYLPQEKVMGGTIASMVAATVLGAVVALAF
                     RD"
     gene            9749..>10102
                     /gene="nrfD"
                     /locus_tag="VHM69_05570"
     CDS             9749..>10102
                     /gene="nrfD"
                     /locus_tag="VHM69_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2739896.1"
                     /translation="MSETTRDQVQQSNGKVETSHHPETDPQRKDNNYYGIPPIKHAHW
                     TWQIPVYFWIGGIGAGVHMFSTIAQILGHEDDDLTRISRYTVLATMMISPVLLIWDLG
                     RPERFYNMLRILKLRS"
BASE COUNT         2037 a         3149 c         3113 g         1803 t
ORIGIN      
        1 tcgtcgttcc tctccacaaa actcctttcc cacactcctc cgattgactg ggcactatat
       61 ggcatgcttt gtatacagtc aagcgtatac gattggtcgt atgtgcttgg gtacacgggc
      121 ggacgaaggc cctccggcac agagtaccgg agggctctgg tagtgagggg ttctaagcct
      181 tcgctccttc cgtttgggtg gcgctggcct cggatttctg ctttggcggg ttaactataa
      241 aggccacgac agcgctgccg accatgatcg ctgcgatgat gtagaaggcg agcgtgtagg
      301 agctcgtcac atcgatgacg cgggagatca ggagcggccc gagcacgcca ccgaagctcc
      361 aggcggtaag catgaggccg tagatcttgc ccacgttcgc agaaccgaag tagtccgccg
      421 cgaaggccgg catcgtcccg aagcccccgc cgtagcagga gatgatagcg aacgagatga
      481 tggcgagcag gacgtacaca gtgccgagga agggcagtag gatgaagagc acagcctgca
      541 ggaggaacat cacgaagaag acccacttgc gcccgatggc gtccgagagc caggcccaga
      601 aaaagcgccc cagtgcgttg ccgacggaga tgatgctgac aagcccggcg gctacggtcg
      661 cgctcacgcc ggcgagcgtc acggccatcg gagaggcgac cgtgatgatg gagatacctg
      721 ccgtgacgtt gaggaaaagc agcgcccaca gcgcgaacca ctgccaggtc ttgagcgccc
      781 cgccaaggtc gtagttcacc tctgagctgt cgccgctctc ggtagtctcg ggttcccagc
      841 cttcgggctt ccagccctcc ggcggattct tcatgaacag cgcggctccg acgaccatga
      901 ccaggtagat gatacccaga atagtgaacg tccggaaagg cccgaccgag gagaccaggc
      961 tcttggccac cagcgccgtc acgaacgcgc caccgccgaa gcccgctacg gctatgcccg
     1021 tgatgaaacc gcgcttgtcg gggaaccatt tgatcagggt ggcgacaggg acgatgtacc
     1081 ctagcccgat gccggcgccg gcgatgaagc cgtacgtcag gtagaggagc aagaggctgt
     1141 tgcttgcgaa accggcaagg aagatcccca gcccgtacaa cacgcccgcg gtaagggcta
     1201 ctgtgcgcgg tccaacgttg cccatccaca gcccgcccac gaaggccgcg aacccgagcg
     1261 tgaagattgt aatgctgaac gtcgagttca ccgcggtcgc ggatgcgcca ccaaacgcct
     1321 gcgcaagcgg gtcggcgaac acgctccatg catagactgc cccgagcgcc acctgcataa
     1381 cgacgcctgc tatggcaatg atcacgcggt tacccgcgcc ggaactgcta actgcttggc
     1441 ttgccacttg actcctccct catgaacctt ccccagaacg aattacgaac gtacttgcca
     1501 gaatcggctg actattgcac ctccccctta cacattcaaa cctgtcccgg caccaaccag
     1561 aatggttctt accctattcg gggagaatcc tatcagaccc ggcgtagaca ttgaatcgcc
     1621 cctcgcgcag gaacccgacg agcgtcattc caaactcctt cgccacgtcg acggcgaggc
     1681 tgctcggggc tgagatggca cacacgatgg ggacgcctgc cgtgaggcac ttctgcagga
     1741 tctcgaagct gctgcgcccg ctgaccatga cgacgtgatc cgagatcggc agcctgcctt
     1801 cgaggagcgc ccatccgacg agcttgtccg tcgcgttgtg tcttcccacg tcttcccgca
     1861 gcgcgataag ctccccgttg ttgtcgaaga gcgccgccgc gtgcagaccg cccgtcacgt
     1921 cgaagagccc ctgcgcctcc cgcagtttct caggcattcc atagatggtc cgtgccgtga
     1981 gcgtcggtcc aggcggaatg atggggcatc ctcgcagttc caactgctcc aggctcgcct
     2041 ttccgcacac gccgcaggcg ctcgtggtgt agaaatgcct ctccagcggg cgcaagtcgt
     2101 actcgcgatt gccacgaagc tcgacgttca cgatgttgta ccgttgttcg gcgtcgaggt
     2161 cggagtcgac gcagtagctg atcttcagta tgtcatcact agagctgact atgccctcgc
     2221 cgtacaggaa ccccgccgca agctcgaagt cggcgcctgg ggtacgcatc gtgacggcga
     2281 cggtctgctt cgtatccccg gcgagcagcc tgatctccat cggctcctcg gtcgctaaag
     2341 tgtcgggacg catgcgcatc cggccttctt ctacgacccg tatccgcgtc ttggtcttgc
     2401 tcccgcgcct gggtttgccc gaggtcttct ccagcatcag cctctcctcg tctgccgaca
     2461 agtgtatcag cgacggcgtg ttagcatgca tagaccatgt acaccggacg gacactttga
     2521 ggcttatggg tctcccgaac gtcgcccgct atcccgaggc gactgtcgtc cgagacgaaa
     2581 cccacatcct gataacgttc ggcggtctcc acggggagca cacgatgggc gtacccctcg
     2641 agtacgtcgg cggggacgag gagattgcgg aactgcggct gctcgcgcaa ttgcagcaca
     2701 tcggctacag ggtcaggcgt ggagaacggg atccctcaga cccgtagcgg cctgaaagct
     2761 gctgagacta tcctacggtg cgggctcgat ctgctttcgt gcccattccc ttatcgtctt
     2821 gagccagatc gtgcgccttg tggccttact cttctcgacg aagccggtaa gtgcggtgtt
     2881 cgtggcgggc gggaggacgg tgctatctgt ggactcggcg tagtcggaac cttcgagaac
     2941 caggatcgtc aggcgctttc catcgtagcg ccagacctcg cagacgccta tctgggcgta
     3001 gatggggagt ttgctgaacg aaggactggt gatgtctatc tcgatcacca ggtccggcgg
     3061 cgggtccaca gccaggtcta tacggtcctt gccgcgaacc tgctcctcgt tctgaatgta
     3121 gaagcaggag tccggctcga atccgcgctc gaggtcttct cgcctgaagg tagtggagcc
     3181 gagatcgtaa acgtcgatgc ccagttccgc agccaggaac tcgacgatta gtgcgatgct
     3241 gcgattgaac gcttcatgct cgggtgaagg gctcattatc tccaactctc cccgatcata
     3301 ggtaaaacgg ggcgcgctgt tgttctcatg gtcggcgagc agacgttcgt aggtattcca
     3361 accgatgttg tgaaggacca ctctttgctc cgccggagtc tttaccgttt ccatcgaata
     3421 ctcttttcga cgcttacgtc atgattctat agcgtacctg agacaaggaa agagggccgg
     3481 agagatccgg ccctctaaaa ccgtcctcga tgtccgaggt gctacgcgtc gatgtagccg
     3541 ttcgggttga ggacgtactt cttggccgcg cccttgtcga agtccttgta gccttgcggg
     3601 gcttcgtcga gggtgatgac ggtggcgttc acggcgtcgg cgatgtgggc cttgtcgtgc
     3661 aggatgctca tcatcaggcc gcggttgtac ctcttgaccg ggcattggcc tgtgtagaag
     3721 gcgtggctct tggcccatcc caatccgaac cggatcttga gcgtaccctc ctgggcgtcg
     3781 gggtccgcag caccagggtc gccggtcacg tagaggccag gaatacccag agcgccgccc
     3841 gcgcgggtga tcgtctggac ctggttgagg accacggccg gggattcttc accggggcgt
     3901 gcagaggcct cgaagccgac cgcgtcgacc gcgcaatcga cctcgggcac gccgaggatc
     3961 tcggagatct ggtcaggcaa ctcggcgccc tcgccgacgt tgaccgtctc gcagccgaaa
     4021 gatcttgctt gcgccagcct ctcgtcgttg aggtcgccga cgatgacgac ggcggcgccg
     4081 agtagctgcg ccgagtaggc cgcagccagt cctacagggc cggcgccggc gacgtagacc
     4141 gtagatcctg tcgtcgttcc agccgtgtat gcgccgtggt agccggtcgg gaagatatcc
     4201 gagagcatgg tgaggtcgag gatcttctcc agtgcctgat ccctatccgg gaacttgagc
     4261 aagttgaagt cagcgaaagg caccatgacg tactccgcct gtccgccggt ccagccgccc
     4321 atgtccacgt agccgtacgc cgagcccgcg cgcgccgggt tcacgttgag acagacgccg
     4381 gtcttgcctt cgttgcagtt acggcagcgt ccgcaggtga tgttgaaggg gacggagcag
     4441 atgtcgccca cgtcgatgaa ctggacgtcg ttgcccttct cgacgacctc gccggtgatc
     4501 tcatgcccga gcgtctgtcc gacgggtgcc gtcgtcctcc cgcgcaccat gtgctggtcc
     4561 gagccgcaga tgttggtaga gacgatcttg aggattgcgg cgtgcggggc ctccttgctc
     4621 atccccatcg cgtccgctac ctcaccagga acttccagct tcgggtactc gatgtcctct
     4681 acggctacct cgccggggcc tttgtagacc actaccctgt tgtcggccat aagctgattc
     4741 tctccttctg tctagccgga gataattgac aatcgtcatt gctttcttcg tcggccacgc
     4801 atgaaaaggc atcttcggcg cggctgcgtg tcagattttc aggcaacctc cttccctctc
     4861 cccgatagtt gctccggttg tcagcccggc aaagtaccat gtgttgcgta gcggcgcaag
     4921 gatgggacat acacattcgc atctcaagtt atgtcgaggt ttcgcgatag gccgaataag
     4981 gttagaatct aaaaaggagg aaacccggac aatacaatct ttcgcgcaga aggggtcacg
     5041 tatgctcttc aaggtgacgg agaaagacgg tctctggaat aagatccgga accacgtcgg
     5101 aacgacggtg aagcacccga taagcccggc gactcgcgac attcgttcgc gcatctccga
     5161 cgatggcgtt gagaagaccg tgagcgtctg tccctactgc gccgtgggtt gctccacgct
     5221 cgtctaccac cgcgacgggc gcatcataga tatcgagggg aaccccgaca gtcccgtaaa
     5281 cggcggcacg ttatgcccca aggggtcagc taccttcggt atgcacgtct cgccgcaccg
     5341 caagacgaag gtcatgtacc gcaggcccta ttccgaccag tgggagcatt tgaccctgga
     5401 tcaggcactc gacatgatcg ccgaccgtgt gaaggatgcc cgcgaggaga cctgggaaga
     5461 gaaggacgag caaggcaaga agctgaaccg ctcgatggcc gtagcctcca tcggcggcgc
     5521 cacgatagac aacgaggaga actacctcat aaccaagctc ttccattctt tagggttcgt
     5581 gcgaattacg aaccaggcgc gaatatgaca cagctccacg gtgcccggtc tgggcatcac
     5641 gtacggtagg ggcggggcca cctccaacct ccaggacctg cagaacgccg actgcatact
     5701 catcgaaggt tccaatatgg ccgaagccca ccccgtcggc ttcaggcacc ccatgatcgc
     5761 caagaagaag ggcgccaagc tcatccacgt cgatccgcgc tttacgcgca cgtcggcgat
     5821 gtgcgacatc tacgcgcctt tgagacctgg caccgacatc gtgttccttg gaggtctgat
     5881 caactacgtc ctggagaacg aggagtactt cgaggagtac gtcaaaatct acacgaacgc
     5941 agccacgatc atcaccgacg acttcgagga cgccgacgca tccggactgt tctcgggctg
     6001 ggacgaggag agcaagcagt acgacccttc gacctggcgc tacgagggac accccataga
     6061 ctacgcctcg gtcggcggga gccagggccg catcgtcggc gaggccggcg agggcgagca
     6121 cggggaaatg cacccaggca tgccgcgccc gacagacatg tcgctacagc acccgcgctg
     6181 cgtgttccag atcctcaagc ggcacttcgc caagtacacg cccgagatgg tcgagaaggt
     6241 ctgcggtacc ccgaaggagg ctttcctcga agtagcgaag accctggccg agaactcggg
     6301 tcgcgagaag acctcttcgc tctgctacgc ggtcggctgg acgcaacact ccaagggcgt
     6361 acaggtcatc cgcacggcgg cgatcctgtg gcttcttttg ggtaacatcg ggcgcccggg
     6421 cggcggcatc atggcgctgc gcggtcacgc gaccatccag ggctcgacgg acatcgccac
     6481 cctctacaat atcctcccag gctacctgcc gatgcctgat accaacaagg accacgacac
     6541 gtggcaggac tacgtcgaga acgaaacggc gtcgagcggc tggtggtcca acttccccaa
     6601 gtacataacc agccagatga aggcctggta tggcgagaag ttcgacgcgg agagcggcgc
     6661 tctattcgac cagttcccgc gtctttcggg agaccactcg tattacccga cgatcatgga
     6721 tatgaaggat ggcaaggtaa ggggagcctt catcttcggg cagaacctcg ccgtcggcgg
     6781 cccgcacgcg aagctcgcgc gcgacggcct caggaacctc gacttcctcg tcgtgctcga
     6841 cgcctacgag atcgagacct caacagtctg gaagttggac ggcgtgaaac ccgaggagtg
     6901 cggcaccgag gtcttcttta tgccgatggc gctcgtggcg gagaaggatg gttctttcac
     6961 ccagactcag cgcatgttgc agtggcacga caagtcagtc gacccgcctg gcgaggcccg
     7021 ctccgacgcc tggttcgttt tccacctcgg acgcaggatg aaggagctgt acaagggttc
     7081 aaggaagcac cgcgacaggc tcataagggc gatgacctgg gattatccga cggagggcga
     7141 gcgcgaagag cctgacatcg agagcgtcct caaggaggta agcggttaca aggtcgagac
     7201 cggcgagcct gtcggaggat tcgccgagct aaaggacgac ggttcgacgg cgtgcggggg
     7261 ttggatctac tccggagtct acgccggtgg cgagaacaag gcccgcaacc gcaagcccgg
     7321 cggtaacccg aacctcgaat ggggctgggc gtggccgatg aaccggcgca tactctacaa
     7381 ccgcgcctcc gccgaccctg aagggaagcc ctggtccgag cgcaagaagt acgtctggtg
     7441 ggacgaggaa gagaataagt gggtcggcga cgacgtaccc gacttccagg ccgacaaacc
     7501 accttcgtac cgccccgacg aggacgcggg cggggtggac gccatccccg gcgacgggcc
     7561 tttcatcatg aacggcgacg ggaagggctg gctcttcgcg cccgtcgggc tcaaggacgg
     7621 tcccctgcca gtccactacg aggcggtcga gtcgccggtt ccgaacctgc tctacgagaa
     7681 agttcagcac gaccccgtgg ccaagacctt cgacaggccg gacaacccgt acaacccgcc
     7741 agaagacccg cgctttcctt acgtcgtcac gacgtacagg ctaacggagc accacaccag
     7801 cggggcgatg agccgttgga tcccctggct caacgagctc cagcccgagc tgttctgcga
     7861 gatcagcccc acgctcgccg ccgagaaggg cgttaaaaac actgactggg tcacgatcac
     7921 gacgagccgc ggcaaggtcc actgcaaggc cctcgtctcc gaccgcatcc ctgtctacaa
     7981 gttcgatggg aaggacctcc acaccatcgg tctcccctac cactggggac cgaacggcct
     8041 cgtaaaaggc gacgtggtga acgacctgat ccccgtctca ctcgaaccga acgtggccat
     8101 ccacgaggcc aaagccttca ccgccaacct cgaagcgggc agggtgtaga ggcgtgggca
     8161 cgaagcagag ggctctggcg ggagccgtga gcgggctcgg ggggacgctc gtcctgtcgg
     8221 gactgcgtga ggcatgggcc aggatcgggc tcgtcttcga gacagcccct atgcaggtcg
     8281 ttgcccgcgt cgaagaactg ggcctggtcg gggatctctc gccggcgggc cggcgcctgc
     8341 tgaccgtcat cgcccacttc gcgtacggcg ccgggacagg tacagccttc ggcctgctgc
     8401 gcagggagac gggtggaccg gaggaagaag cctctgttgg ttcggccctt ggcatactgg
     8461 cctggggagc gggatgggca agctggctgc cgctcaccgg cgtccacgag ccgccctgga
     8521 ctcagcgcac gcccagggtc ctgctgcccg tgatagacca cgcggtcttc ggcgccgcgt
     8581 ggggacttct ctactggaga cttataaggg ggcgcgtgtg agcgtcgagg agatccgcag
     8641 tagggagatg atggagcagg agttgatgga cctcggggtg tggctcatcg agcatcccga
     8701 cgtggtggag aagaacctca agcccataag gagcgcgaag attctcgaac cgggcgtcta
     8761 ctacaacatg ggcacgggga tggtcgacag gatctacagt ccacagcgcg tcgcgctcgg
     8821 gcacaagatg ttccgcataa ccagagatcc ggcgtcgccg gtcgaggaga tccggcgcat
     8881 tgcgctagaa gggaagggct agtggcgaca ggattcctga ccgacacaac ggtctgcatc
     8941 ggatgcaagg cctgcgaggt cgcatgcaag cagtggaacc agctccccgc cgacggctac
     9001 gagctgacgg gcgactcgta cgacaacacc aagcagctct cgggcacgac ctggcgccac
     9061 gtcgcgttca tagagaaccc gaagaagcgc cgcctgacag gcgacgtcga cctcgtcgcc
     9121 ctcgcggagg acgtgacgga acaactgcca ggcggggccg agctcgacgc cctcggacga
     9181 tgggcgttca tgagcgacgt gtgcaagcat tgcgtcgagg cgccgtgcca gcaggcgtgc
     9241 ccgacgggct ccatcatccg caacgagttc gacaacgtct acatccagcc cgacatctgc
     9301 aacggctgcg gctactgcgt ccccgcctgt ccctacggcg tcataaccca ggtcccccac
     9361 gacgggcgag ccttcaagtg caccctctgc tacgaccgcc agaaggacgg cctcgaaccg
     9421 gcctgcgcca aggcgtgccc gaccaactcc atcatgttcg gcgaggtcga ggagctgaag
     9481 cagattgccg aggagcgggt gcaggagctc cacgacaagg gctacagcca ggcctacgtc
     9541 tggggcacgc ccgaggtggg ggggaccggg ggcataaagg gcaaccactc caagttcatc
     9601 ctcatggacg agcccgaggt ctacaacctg ccccgtcatc cctacctgcc gcaggagaag
     9661 gtcatgggag gaaccatagc aagcatggtc gctgcgacgg tgctcggcgc ggtagtggca
     9721 ctggctttca gggactaggg ggagacggat gagcgagacg accagggatc aggtccagca
     9781 gagcaacggg aaggtcgaaa cttctcacca tcccgagacg gacccgcaga gaaaggacaa
     9841 caactactac ggcatcccgc ccataaagca cgcgcactgg acgtggcaga tccccgtcta
     9901 cttctggatc ggcggtatag gggccggggt ccacatgttc tcaacgatag cccagatcct
     9961 cggccatgaa gacgacgatc tcacccgcat aagccgctat acggtgctcg ccaccatgat
    10021 gatcagccct gtgcttttga tctgggacct cgggcgtccc gagcgcttct acaacatgct
    10081 ccgaatcctc aagctccgtt cc
//
LOCUS       DATAIR010000233        15946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_291856, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000233 DATAIR010000000
VERSION     DATAIR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291856"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="dhaK"
                     /locus_tag="VHM69_05575"
     CDS             <1..947
                     /gene="dhaK"
                     /locus_tag="VHM69_05575"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX2739897.1"
                     /translation="ERAHGGLIRLIPEHNVVVRADAPVQGKVALVSGGGSGHEPTHGG
                     YVGPGMLDAACAGAVFTSPTPDQMLEATKAVDGGAGVFYVVKNYTGDVLNFEMAGELA
                     EAEEIETDYVVTNDDVAVEDSTYTSGRRGIAGTIFVHKICGAAADDGKNLAGIKELAE
                     RVNSNVRSMGMALTSCTPPESGEPIFDIGEDEMEIGIGIHGEPGIERKQVEPADRIVD
                     ELLAKILGDSVDYSGSEVAVMVNGMGGTPLMELYVAYAHVADVLDREGIQVWRMPYVG
                     DYMTSLEMAGFSITLLKLDDEMKGYLEAPCDVASGRPF"
     gene            961..1698
                     /locus_tag="VHM69_05580"
     CDS             961..1698
                     /locus_tag="VHM69_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX2739898.1"
                     /translation="MQAYLGEFIGTMILIILGDGVVAGVLLRNSKAENSGWIVITFGW
                     AMAVAVAVYCVGQFSGAHINPAVTIAFAATGQFEWAMVPGYIIAQFLGAFTGGVIVWL
                     AYLAHWGVTEDAGLKLGVFCTAPAIYNTPANIITEIIGTFVLVFGVSGIVANAGAVPG
                     DLSAVIASGLNPLLVGLLVLGIGLSLGGPTGYAINPARDLGPRIAHAVLPIAGKGGND
                     WGYSWIPVVAPIIGGILGAVTFSVLFG"
     gene            1766..2533
                     /locus_tag="VHM69_05585"
     CDS             1766..2533
                     /locus_tag="VHM69_05585"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2739899.1"
                     /translation="MAVLDAFDLTGKTAVVTGGNTGLGEGFAKALAEAGAGVALAART
                     REISETVAKEISDKGGTAIVVDLDVTDPVQVDTALDEVTERLGPVDILVNNAGACYHR
                     PAVEVPLEEWHDVFDVNVHGLWHCAQAVGRRMIQGGGGSIINVGSISAMIVNRPQWQP
                     AYNASKAAVHQLTKSLAAEWAPHNVRVNALAPGYVKTAMAPVDEPRFRRHWIEDAPMQ
                     RYATPEELGPSIVYLASDASSFMTGSILVVDGGYTLF"
     gene            2555..3304
                     /locus_tag="VHM69_05590"
     CDS             2555..3304
                     /locus_tag="VHM69_05590"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2739900.1"
                     /translation="MEEFSLQDRVAVVTGGGQSLGLAISRSLHSAGAEMVIAEVNDET
                     GPEAAEELKGMFVHTDVTDPASVRGMVETVIDEHGRIDVLVNNAGIVHNIPSEEVPDE
                     EWLAVISVNLNGVFWCCREVGKAMLQRGSGSIVNIASMSGIVSNHPQPQAAYNASKAG
                     VITLTKSLAGEWASRGVRVNSVSPGYIRTPLTELGMSKTEWAEVWLDSTPLGRLAEPQ
                     EIAPAVLYLASDAASFATGTNLVVDGGYTSW"
     gene            complement(3652..4245)
                     /locus_tag="VHM69_05595"
     CDS             complement(3652..4245)
                     /locus_tag="VHM69_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739901.1"
                     /translation="MQRLILLLSLMAVTLVVTSGVALAVARSGGPGDDTLRGTNGPDA
                     LSGNGGDDVLLGLRGRDALDGGPGRDAVLGGNEFGPLEGDRSLDGGDGGDFVGSGLGS
                     DAMAGGPGRDFLLDAEFEVGHQDVISAGSDDDAIDAIQRPAARDVIDCGSGFDGVLVD
                     SKDITSGCERIFTRVNRFYGRFLENSNYNYFEPLNRL"
     gene            complement(4650..5816)
                     /locus_tag="VHM69_05600"
     CDS             complement(4650..5816)
                     /locus_tag="VHM69_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX2739902.1"
                     /translation="MSRQGHLIAVVGAILIAFVTVACGGGGQGNAAGRDSERDPATNE
                     ETAASKVDRSTAKTTREPVGLDVETVTPGKAYEPAGFVEGSLWATDIVACNDTGSASS
                     SAGSYDSTVNGSAPVSLVACAAPKKTLLKRLDPRTGEEVAEIPLKFFYADSTRVGVGA
                     GSVWVSGAGVVTRIDPETNRVVDRIPVDSPTGLAFGHGSVWVSSAQNGTVSRVDPQTG
                     KVAAKIEVGEGAVDIATDESSGDVWVACSYQAEDNKLSRVDPATNRVVAEIPIEAHSR
                     YGGAWRVALGEGVVWAQSVDGRLFKVDPATNEVAAVVPLGGYSSDLAVYGGAVWATAQ
                     PSSGTRLYRIDPSTARVVATEDGPEPATGGYRGLVAGGGYVWLASGGGLARVAP"
     gene            complement(6069..7307)
                     /locus_tag="VHM69_05605"
     CDS             complement(6069..7307)
                     /locus_tag="VHM69_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739903.1"
                     /translation="MSSRTASRLAWSVCALSLALIAATLVLIFLGWSTRLPSGWTTWQ
                     EQALSVAGFIGAPIVGGLIASRRPANIYGWLWLGLGMAQALLQFGQAYAAYALVVDPG
                     SLPAPRTLVTVLGQGWIASVVIIPFLWLLFPDGRLPSPRWKVVAWTVVLVGVLLLLVA
                     PLAPGKSGTAPAGNPIGMEGAVGRTIGALVDPGVLVLFVAIVLSALSLVFRYRRASGI
                     QRQQIKWFAYAAALNGCLVTIDILDFSQYLGDALWTLLGSLAFASLYAAVGIAVLRYR
                     LYDIDVVINRTLVYGLLSLTLAGVYFGGVAATQALLSASTGQEQLPQLAIVVSTLVIA
                     ALFNPLRRRIQSFIDRRFYRRKYDARKTLQEFSTRLREETDLDALSDEMVGVVRETMQ
                     PEHVSLWLRPAISQKGERPK"
     gene            7538..8113
                     /locus_tag="VHM69_05610"
     CDS             7538..8113
                     /locus_tag="VHM69_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739904.1"
                     /translation="MALRRLLLLGTPLVLAPLMVVHQLIDQFERPEAFLALHLILLPL
                     FALMGVAIWELLAGIGGAVAWTARAAAFIFIVGYVALDAISGIAASAILASGAPWSAE
                     AARALFASGPAALPTTVAVLAWRLSVLLTAWALYRNGRPLAPLVLLLATALWLNNDHG
                     GLRGVVVFGCFALAVAWLEFSSGAPRRPVRG"
     gene            8391..9464
                     /locus_tag="VHM69_05615"
     CDS             8391..9464
                     /locus_tag="VHM69_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739905.1"
                     /translation="MLLQRRSSILRPFALLFVLVAGLAVAHVATLSAAQAAPLGGLKQ
                     FRVPTADSAPRHITVGSDRNLWFTEGNEIFTPDPDPDMGGTFHRNIGMITPAGDITEF
                     RIESGIEPNQCFCSPNDIVQGPDGILYFTTNDPGLGRITTEGEVLPFVAPDNSLANGG
                     GIAAHLDDIWYTDFNNDSIWRYDIPSGEFTEFPVTPGATPVDVAVASDGFVWFTERGD
                     GAIGRLDPQSGAFTETSVPGGDAALLSQIAIAPDGSVWFTELLGHAVGRLVPATNQVT
                     EFPTPGVGPEGIVATQGGVLWFTQAVTGNIVRITPNADGTITFSEGKIVKNSNPLGIT
                     IGPDGNPWYAMESANKIATLQLR"
     gene            9642..10907
                     /locus_tag="VHM69_05620"
     CDS             9642..10907
                     /locus_tag="VHM69_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739906.1"
                     /translation="MSRRSAAWLAWSLWALSVGLTALSLVLLVLIRSHPNVHIFDWWL
                     GNSLVVIDVTVGAIVASRRPENLVGWLLCLFGVAVGTSSFCSQYAIYAMLAKPGELPA
                     GEALAWVAAWLLPILISLQVFYLLLFPTGRLPSRRWRWLVWMTAAFIVVGVVTSAFAP
                     GAYFGALGSIRNPLGIEGFTNLYRAVLYTISPVLFAAAALSVLMRLRRAVGVERQQIK
                     WLAYAAAIYAMSILLNIVNSAVDTPLWFERTGAAIFTAAGLAIPVAIGIAILRYRLYD
                     IDILINQTLVYGLLTATLIALYFGGVTLLQSLLGSLTGQESQLAVVVSTLVIAALFNP
                     LRRRIQSFIDRRFYRRRYDARRTLEAFHARLREEVDLDSLAANLVSVVKDTMQPAHVS
                     VWLRPDPDSERFDRVESEPKGAEKQVRVS"
     gene            10904..11746
                     /locus_tag="VHM69_05625"
     CDS             10904..11746
                     /locus_tag="VHM69_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX2739907.1"
                     /translation="MKEAGRKVRFEEMFPWEIAPAMAEAPLCYLPLGTLEWHGEHAAV
                     GLDALKAHAVCVLAAQRSGGLVVPPLYWSTDWREDLTDGGYLTGGIERGERYHVPGNM
                     FWLRQETFHNLLLDIYEAMRRRGFLAIVVLSGHWSIDHNIAAIRETGERFLRAHPAMG
                     WLLLTDQEVVPDLHYPVEHAAGGETSLLMAIRPDLVAIDKTFETDRSLRPNYAGEPDH
                     LRRRRETPHRYIGVNPAVEDASNDPEGATTERGRLLLDTIVERVALRASSLLAEVTDD
                     DEPA"
     gene            complement(11762..12721)
                     /locus_tag="VHM69_05630"
     CDS             complement(11762..12721)
                     /locus_tag="VHM69_05630"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX2739908.1"
                     /translation="MSRLYSAGNNAVGVIENGGVRLSLEGRGARCVAVDPEDPDTLYV
                     GTSDEGLFKSEDGGETWQKLSGIEHSRVTAIAVSPADGAIYAGTEPSTLFVSRDGGGS
                     WRELEDMKKLPSAPTWSFPPRPWTSHVRAIALSYTDPDLVVAGIELGGVLRSTDGGET
                     WQDQRPGAQADCHSLVAHPEDPRLLYESGGGGFARSRDFGDSWEAADEGMRLHYAWGL
                     AVDAEDPSLVYASAAPDPWRAHGRGFSGAAIYRRTPDERWEPLLEDLAAFPYALCPDP
                     VEPGTLYAGFGDGKVLRSQDTGQSWDEIGRVPSGLAALAAVAA"
     gene            complement(12872..13105)
                     /locus_tag="VHM69_05635"
     CDS             complement(12872..13105)
                     /locus_tag="VHM69_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739909.1"
                     /translation="MSEQARKVKKEAIPQWAEGLYYNKGLFSEVLVYYDDEAVYFECR
                     GKDSGIVRFTFEMLQTLTTLIEEEQEEQEKTSE"
     gene            13426..13794
                     /locus_tag="VHM69_05640"
     CDS             13426..13794
                     /locus_tag="VHM69_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2739910.1"
                     /translation="MSMQDPGSALDFEVLRGAIEDRDAETLLSLYADDAEVRTINRNT
                     TPSSPLVLRGKEEIGEYLRDVSSREMTHKVENEVVGTDRISFQEACEYPDGVRVLGAE
                     TLDLRNGKIVRQVNVEAWDE"
     gene            14406..14591
                     /locus_tag="VHM69_05645"
     CDS             14406..14591
                     /locus_tag="VHM69_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739911.1"
                     /translation="MDHHLQGGQLHGLRQGGRADEPAQTVANRARWGDFLAFLAVPHV
                     LDEVANFHEQICPEELA"
     gene            complement(14648..15676)
                     /locus_tag="VHM69_05650"
     CDS             complement(14648..15676)
                     /locus_tag="VHM69_05650"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2739912.1"
                     /translation="MHNRAAVMYGTHDVRLEEVPVPEPGPKEVLVEIRAVGVCGSDVH
                     YYEEGRIGSFVVREPLILGHESMGTVVALGDGATKHEVGERVTLEPGVPDGTCRECRA
                     GRYNLCPNVRFFATPPIDGAFTNYVSIHEDFAYSLPDELSDNAGALMEPLSVGIWACK
                     KGEVTAGDHVLVTGAGPIGLLAMQVAFAQGATEVTVTDVADERLQIARRLGATRAMNV
                     AEEPLDEASVEADVLIECSGNERALGDGIRCVRPAGFVVVVGMGPNEETSVPLAFIQT
                     REITLTGTFRYANTYPTAIDLAATGKVDLDAMVTSHYGLDDTEEALKASRQDPANVKP
                     MVLPDGTS"
     gene            complement(15691..>15946)
                     /locus_tag="VHM69_05655"
     CDS             complement(15691..>15946)
                     /locus_tag="VHM69_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739913.1"
                     /translation="PGIVTTAILCLVFSWNDYALAATFAGPNSQTLPIAAAQLNTQTG
                     LSWGQLSAIGTIVVLPMIIVGLAVRRYLVRGLTLGAVTGE"
BASE COUNT         2972 a         5066 c         4952 g         2956 t
ORIGIN      
        1 tggagcgtgc ccacggaggc ctgatccggc tgatccccga gcacaacgtc gtcgtacgcg
       61 ccgacgcccc cgtgcagggc aaggtcgccc tcgtctcagg cggcggctcg ggccacgagc
      121 cgacccacgg cggctacgtc ggtccgggca tgctcgacgc ggcgtgcgcg ggcgccgtgt
      181 tcacgagccc tactcccgac cagatgttgg aggcgacaaa ggcggtcgac ggcggagcag
      241 gagtcttcta cgtcgtcaag aactacacgg gcgacgtcct caacttcgag atggcgggag
      301 aactggccga ggccgaggag attgagacgg actacgtcgt aaccaacgac gacgtagcgg
      361 tcgaggactc cacgtacacc agcgggcggc gcggcatcgc aggcaccatc ttcgtgcaca
      421 agatctgcgg cgccgccgca gacgatggca agaacctcgc cggcatcaaa gagctcgccg
      481 agagggtgaa ctccaacgtt cgctccatgg ggatggcgct cacgagctgt accccgcccg
      541 agagcggaga gcccatcttc gacatcggcg aagacgagat ggagatcggc atcggcatcc
      601 acggcgagcc aggcatcgag cgcaagcagg tcgagccggc ggaccggatc gtggacgagt
      661 tgctcgccaa gatcctgggc gactccgtgg actactcggg ctccgaggtg gcagtgatgg
      721 tcaacggcat gggcggcacg ccgctcatgg agctctacgt cgcctacgcc cacgtagcgg
      781 acgtcctcga ccgcgagggc atacaggtct ggcggatgcc ctacgtcggg gattacatga
      841 cctcgctcga gatggccggt ttctccatca cgctcctgaa gctcgacgac gagatgaagg
      901 gctacctcga agcgccgtgc gacgttgctt ccggacgacc attctagaaa ggagggatag
      961 atgcaagcat atctcggtga gttcatcggc accatgatcc tgatcatcct cggcgacggc
     1021 gtggtggccg gggtgctgct caggaattcc aaggccgaga attccggctg gatcgtcatc
     1081 accttcggat gggcgatggc cgtggccgtg gccgtctact gcgtcggcca gttctccggc
     1141 gcccatatca accccgccgt gaccatcgcc tttgccgcaa cgggccagtt cgagtgggcc
     1201 atggtacctg gctacataat cgcgcagttc ctgggcgctt tcaccggcgg cgtcatcgtg
     1261 tggctcgcgt acctggcgca ctggggagtg accgaagacg ccggcctcaa gctcggcgta
     1321 ttctgcacag cgccggcgat ctacaacacc cccgccaaca tcatcacaga gatcatcggc
     1381 accttcgtgc tggtgttcgg tgtttcgggc atagtcgcaa acgcgggggc agtccccggc
     1441 gacctctcgg cggtcatcgc gagtggtctt aatccgctcc ttgttggcct gctcgtcctc
     1501 ggcatcgggc tttcgctggg cggtcccacg ggctacgcca tcaacccggc gcgcgacctt
     1561 ggcccgcgca tagcccacgc cgtcctgccg atagcaggca agggcggaaa cgactggggg
     1621 tactcctgga tcccggtcgt agcccccata atcgggggta ttctcggcgc ggtaaccttc
     1681 agtgtcctgt tcggatagcg ggttttgacg atgtcgtggg gagcgcgcac tccccacggc
     1741 gctctcatgt aggaaggatg acccagtggc cgttctcgac gcgttcgatc tcacaggcaa
     1801 gaccgccgtc gtgaccggtg gcaacacggg cctcggagag gggttcgcca aggcgctcgc
     1861 agaagccggc gcgggcgtcg ccctggcagc ccgcacccgc gagatctccg agacggtggc
     1921 gaaggaaatc tcggacaagg gcggcacggc tatcgtggta gatctcgacg tgaccgaccc
     1981 ggttcaggta gacacggccc tggacgaagt aaccgaacgc ctcggaccgg tagacatcct
     2041 ggtgaacaac gctggtgcct gctaccacag gcccgctgtc gaggtgccgc tcgaggaatg
     2101 gcacgacgta ttcgacgtca acgttcacgg gctctggcac tgcgcccagg cggtggggag
     2161 gcggatgatc cagggcggcg gaggctccat tatcaacgtc ggttcgatct ccgccatgat
     2221 cgtcaaccgt cctcagtggc aaccggcata caacgcctcg aaagccgcgg tccaccagtt
     2281 gaccaaatcc ctggcggcgg agtgggcgcc gcacaacgtt cgcgtgaacg ccctggcgcc
     2341 tggctacgtc aagacggcga tggcccctgt ggacgagccg cgcttcaggc ggcactggat
     2401 cgaggacgcc cccatgcagc gctacgctac gccggaggag ctgggtccga gcatcgtcta
     2461 ccttgcgagc gacgcctcca gtttcatgac aggctccatc ctggtcgtgg acggtggcta
     2521 cacgctgttt tgagcgtcag aacggaggga tgagatggaa gagttctctt tgcaggacag
     2581 ggtcgctgtg gtcaccggcg gcgggcagag cctgggactt gcgatctcac gctcccttca
     2641 cagcgccggc gcggagatgg tcatcgccga ggtcaacgac gagacaggtc cggaagccgc
     2701 cgaggaactc aagggcatgt tcgttcacac cgacgtaacg gaccccgcct ccgtgcgggg
     2761 tatggtagag acggtcatcg acgagcacgg tcgcatagac gtactcgtca acaacgccgg
     2821 gatcgtccac aacatacctt ccgaagaggt ccctgacgag gagtggctgg ccgtcatctc
     2881 cgtcaatctc aacggcgtgt tctggtgctg ccgcgaggtc ggcaaagcta tgctacaacg
     2941 cggctcaggc tccatcgtca acatcgcctc gatgtccggt atcgtctcga accaccccca
     3001 accccaggct gcatacaacg cctccaaagc aggggtcatt acactcacaa agtccctcgc
     3061 gggcgaatgg gcctcgcgcg gcgtcagggt caacagcgtc tcgcccggct acataaggac
     3121 gccgctcacc gagctcggca tgtccaagac cgagtgggcc gaggtgtggc tagattcgac
     3181 tccgctcgga cgtctcgccg agccgcagga gatcgcaccc gccgtcctct acctcgcctc
     3241 cgacgcggcg agcttcgcca cgggcaccaa cctcgtcgtc gatggaggct acacctcctg
     3301 gtgagaaaca ggtccagtag atgccaggcg gcgggaagca gatatcctgg catgcgtaag
     3361 tggccttctc gtggttggga cggaacagac ggtacgtaag gccgtgcgac cggtggagcg
     3421 ttcgtctacg cgcccatgat tgcccgatag taagacggtt ggcccgctcg cgaagctttt
     3481 tcagacactc tccgcacgct cgccacccat gccctctagg tgatcctgat tcgccccctg
     3541 cccttctcgt ataggaatgg ctaatgtcga gctctgggca ctcggatgaa taggctagag
     3601 gaagggccgg aaaagctgac ccttcctcgg aagaacaggg gaaacatcaa actacagtcg
     3661 gttcagaggt tcgaagtagt tgtagttcga gttctcaaga aagcggccgt aaaatctatt
     3721 gactctagta aatatccttt cacagccgct cgtaatgtcc ttgctatcca ccaatacgcc
     3781 gtcgaagccg cttccacaat cgatgacgtc cctggcagcg ggccgctgta tggcgtctat
     3841 cgcatcgtcg tcgctaccgg ctgagatgac gtcctgatgt cctacttcga attcggcgtc
     3901 cagaagaaaa tccctgccgg ggccgccggc cattgcgtca gaaccaaggc cgctgcctac
     3961 gaagtcgccg ccatcgccgc cgtcgaggct gcgatcaccc tccagaggac caaattcgtt
     4021 tcctccgagc accgcatccc tgccaggccc cccgtccaat gcgtcgcggc ccctgaggcc
     4081 caacaggacg tcgtcgccgc cattaccgga gagggcatcg ggaccgttgg ttcccctcag
     4141 agtatcgtcg cctggtccac cggagcgggc aaccgcaagt gccaccccgc tggtcacgac
     4201 caacgtcacg gccatcagcg acaacagaag gatgagcctt tgcatcctta gctcctttcc
     4261 tctagaagcg cgtgtaacac cacgccgtcc gttgtgacga tagcagcata cttcccgagt
     4321 gagagcagcg gatcacccaa atgagtgatc tttgagaacg tcctttgtag cgaacagttc
     4381 atatcatgag agggaccgac gctcatcgag gtttcggttt ccctctgcag gttgtcgtgc
     4441 gttttggaaa accgggttac gctactcctg gggtcatgca acggtactct tcatctcgca
     4501 cctgctcgac acacgttctt gatccctggt gtcttcagtg tgttcttgtt cttcctgcgg
     4561 agcttccgag cgcactcctg aatagccaat caccataagg aataccatca ggaggaggac
     4621 gcataagagc tttgaggtgc cttgacgaat cacggcgcga cccgagccag ccctccgcca
     4681 ctcgcgagcc agacgtaccc tccgcccgct accagccctc tgtagccgcc agtggctggc
     4741 tcgggcccgt cttcggtggc gacgacgcgc gccgtgctgg ggtcgatacg gtagagacgc
     4801 gttccagaag agggttgggc cgtggcccac acagcacccc cgtagaccgc gaggtcggag
     4861 gagtacccgc ccaagggcac gacggcggcg acctcgttgg tagcagggtc caccttgaag
     4921 agcctgccgt cgacgctctg cgcccagact actccttcgc ccaaggcaac gcgccacgcc
     4981 ccaccgtacc gcgagtgcgc ctctatcggg atctccgcca ccacgcggtt ggtcgccggg
     5041 tccacccgcg agagcttgtt gtcctcggcc tggtacgagc acgcgaccca cacgtccccc
     5101 gagctctcgt cggtcgcgat gtccaccgcg ccttcgccga cctcgatctt tgccgctacc
     5161 ttgccggttt gggggtctac acgcgacacc gtgccgttct gtgcgctact tacccacaca
     5221 gaaccgtgcc cgaaggccag gccggtgggg gagtccacag gtatgcggtc cacgacccgg
     5281 ttagtctccg ggtcgatccg ggtcacgacg cctgcgccgg agacccacac cgatcccgcc
     5341 cctaccccca cccgcgtgga gtcggcgtag aagaacttga gcgggatctc cgccacctcc
     5401 tcgccggtgc ggggatctag cctcttgagg agcgttttct tgggcgcggc gcaggcaacc
     5461 aaagaaacag gggcggagcc gttcacagtg gaatcgtacg agcccgcaga agaagaggcg
     5521 gagccggtgt cgttgcaggc gacgatgtcg gttgcccaca gggatccctc aacgaagccc
     5581 gcgggctcgt aggctttgcc aggagtgacc gtctccacgt ccaggccgac gggctctcgc
     5641 gtggtcttgg ccgttgatcg gtctactttt gacgctgcgg tctcttcatt ggtggcgggg
     5701 tcacgctcgc tatcgcgccc ggcagcgttc ccctgaccgc cgccaccgca ggccaccgtc
     5761 acaaaggcga tcaggatggc acctaccact gctatgagat gtccttggcg actcaaagcg
     5821 cacccgcctt ctgcatgtgt tctctgctac caggatctcg aagcatgcca cacgacgaag
     5881 ccaggcgcat ttgtagcaag gcgcattcac tgagaaacgg tccaataacc tcgcaagttg
     5941 cggcttacgg ttacaggccg ccgaaacgag atatacaggc cgtgtcgccc tgcattgacc
     6001 aaccggtctc ggagcgtcgt ccgaggcccg ttcgatttct cagcgagatt cgactaacga
     6061 gccggaaact acttcggtcg ctcgcccttc tgggagatag ccggacgcag ccatagcgag
     6121 acgtgctcag gttgcatcgt ctccctcacg acccctacca tctcgtcgct caatgcgtcc
     6181 aggtcggttt cctccctgag cctggtcgag aactcttgga gggtcttcct cgcatcgtac
     6241 ttgcgtctgt agaagcgacg atcgatgaaa gactggatgc ggcgtctcag agggttgaac
     6301 agcgctgcta tcacaagtgt ggagacaacg atggcgagct gcggcagttg ttcctggccg
     6361 gttgacgcgc tcagcaatgc ttgcgtcgcc gcaactccgc cgaagtacac ccctgccagc
     6421 gtcaggctca gtaatccata gacgagggta cggttgatga ccacgtcgat gtcgtagagt
     6481 cggtatctca agacggcgat accgacggcc gcatagaggc ttgcgaaggc taacgagcca
     6541 agaagtgtcc acaatgcgtc gccgagatat tggctgaagt ccagtatatc gatcgtgacg
     6601 aggcacccgt tcagggcggc ggcgtaggcg aaccacttga tctgctggcg ctggatgccc
     6661 gaagcgcgtc ggtaacggaa cacgagcgag agcgcagaga gaacgatggc gacgaagagc
     6721 accagcactc cgggatctac cagggcgccg atcgtcctgc ctactgctcc ctccatgccg
     6781 atggggttcc ctgcgggcgc cgtaccgctc ttacctgggg ccaggggcgc aacgagcagt
     6841 aataggaccc caaccaggac gactgtccac gccacgacct tccagcgggg tgaaggtagc
     6901 ctaccatcgg ggaacagcag ccagaggaag ggaatgatga ccacggaggc aatccacccc
     6961 tgtcccagga ccgtgacgag cgtccggggg gcaggcagag atccagggtc aacaaccagg
     7021 gcgtaggccg cgtaggcctg gccgaactgc aagagggcct gggccatccc caggccgagc
     7081 cacaaccacc cgtagatgtt ggcaggtcgt cgagaggcga tcagacctcc cacgataggc
     7141 gctccgatga acccggcgac ggagagggcc tgttcctgcc atgtggtcca gcccgaaggc
     7201 agccgcgtgg accatccgag gaagataagc accagggttg ccgcaatgag cgccaggctc
     7261 aaggcgcaca cagaccatgc caatcgggag gcggtgcggc tgctcatctc ccgactcctc
     7321 gttcctgttg gattgcccct tctgtcacaa cacaaccact ctacagtaag accgtccccc
     7381 aaacgttacg gcgcccgtta cgaggacgag cttatgggta agggcgaacg tcggagagca
     7441 ccacttcttc gggtgcgccg tgcttcgcca ggtggcatca tgctcatgca gaaatggatg
     7501 gcccacccgc cgaggcggga gtaaggagga gcgactcttg gcattgcgcc gcctgctgct
     7561 actcgggacg ccgctggttc ttgcgccgct catggtcgtc catcagctca tcgaccagtt
     7621 cgagcggccg gaggcgttcc tggcgctgca cctgatccta cttccgctct tcgcccttat
     7681 gggcgtcgcg atctgggagc ttctagcggg catcggcggc gccgtggcct ggacggcgcg
     7741 cgcggcggcc ttcattttca tcgtaggcta cgtcgccctt gacgcgatct ccgggatagc
     7801 tgccagcgcg atactcgcct cgggggcgcc gtggtcggcg gaggcggcaa gggcgctgtt
     7861 tgcctcaggg ccggcggccc tccccaccac ggtggcggtc ctcgcctggc gcctttcggt
     7921 gctcttgacc gcctgggcgc tgtacaggaa cggtcggccg ctcgcgccac tggtcttgtt
     7981 gttggcgacc gctctgtggc tgaacaatga tcacggcggg ctgcgcgggg tcgtcgtctt
     8041 cggctgcttc gcgctggccg tggcgtggct ggagttctct tccggcgcgc cgcgtcgacc
     8101 cgtgcgcggg tgactcccta gtactgcgcc acaggttcat ccgagatctt cggaggaagc
     8161 aggttgagcc tgtctaaagg gtagcggcac atggattcag ccgtacgtgc atgggtccgt
     8221 aacgttgcga agcacggcaa agtataacga gaccccatcc ggcagatgcg ccctgcacgt
     8281 ataagaccgt agaaagagac gctggagttg ccggtcgccg tagaggagag ggaaggagcg
     8341 ccacgacgcg gcagggaacg cgcccgcttg tggccatgga aggaggaaga atgttactgc
     8401 aacgaagatc gtccatcctg cgcccgttcg cgctgttgtt cgtcctggtg gcggggcttg
     8461 ccgtggcgca cgtcgcgacg ctctcggccg cccaggccgc tccgctcggg gggctcaagc
     8521 agttccgcgt acccaccgcc gacagcgcgc cgaggcacat caccgtcggc tccgacagga
     8581 acctctggtt caccgagggc aacgaaatct tcacgcccga ccccgaccct gacatgggag
     8641 ggactttcca ccgcaacatc ggaatgatca cgccggcggg cgatatcacg gagtttcgca
     8701 tagagtccgg catcgagccc aatcagtgct tctgctcacc gaacgacatc gttcaggggc
     8761 cggatggcat cctgtacttc accaccaatg accctggtct gggacgcata acgacagagg
     8821 gagaggtcct cccgttcgtg gccccggaca actccctcgc caacggtggc ggcatcgccg
     8881 cccacctcga cgacatctgg tacacggact tcaacaacga cagcatctgg cgctacgaca
     8941 tcccatccgg ggagttcacg gagttcccgg tcacacccgg cgccacccct gtcgacgtgg
     9001 ccgtcgcctc ggatgggttc gtgtggttca ccgagcgcgg tgacggggcc atcggcaggc
     9061 tagatcccca gagcggcgct ttcaccgaga cgtccgtccc tggcggtgac gccgcccttc
     9121 tcagccagat cgctatagcc ccggacggca gcgtgtggtt caccgagctc ctcggccacg
     9181 ccgtagggcg cctcgtcccg gccacgaacc aggtgacgga gttccccacg cccggcgtcg
     9241 gtcctgaagg catcgtcgcg acgcaaggcg gcgtcctgtg gttcacacag gccgtcaccg
     9301 gtaacatcgt gaggatcacc cccaacgcgg acggtacgat cacgttcagc gagggcaaga
     9361 tcgtcaagaa cagcaacccc ttagggatca ccatcggccc cgacggtaac ccgtggtacg
     9421 cgatggaatc ggcaaacaag atcgccacac tgcagttgag gtaggcgagt agaggacggg
     9481 caacaaaggg ccggggacgc gcaggagcct cggccctttt tctcgccttc ctttccacca
     9541 cactattccg taacgattgc cgtaacgttc gagagacgca cgcggtgtaa agtggaatcg
     9601 tgaccgcggc gaccagagaa caggggcgag ggatcggggg gatgagccgt cgcagcgcag
     9661 cctggctggc gtggtcgctt tgggcgctct ccgtgggtct gaccgcgcta agcctcgttc
     9721 tcctggtcct gatccgctct cacccgaatg ttcatatctt cgactggtgg ctcggcaact
     9781 cactcgtcgt gatcgacgtg acagtgggcg ctatcgtggc ctcccgccga cccgagaacc
     9841 tcgtgggttg gctcctctgc ctcttcggcg tcgccgtcgg cacgagcagc ttttgttcac
     9901 agtacgcgat ttatgccatg ctggcaaagc ccggcgagct acccgcaggc gaggcgctgg
     9961 cctgggtagc cgcctggctc ctgccaatac tgatcagcct ccaggtgttc tacctcctgc
    10021 tattccctac tgggcgattg ccgagcaggc gctggaggtg gttggtatgg atgaccgcgg
    10081 ctttcatagt tgtcggagtg gtcacttctg cattcgctcc cggggcgtac tttggcgcac
    10141 ttggctcgat acgaaacccg ctcgggatag aaggcttcac caacctttat agagcggttc
    10201 tgtacacgat ttctcctgtt ctgttcgccg ccgcagccct atcagtgcta atgcgactgc
    10261 gccgggccgt aggggtagag cgccagcaga tcaaatggct cgcttacgcc gcggcgatat
    10321 acgctatgag catcctcctt aacatcgtca actcggcggt agacacacca ctctggttcg
    10381 agaggaccgg agcagctatc ttcacagccg caggcctggc tattcctgtc gccatcggga
    10441 tagccatcct gcgctacagg ctctacgaca tagacatcct cataaaccag accctcgtct
    10501 acggcttgct tacggcgacg ctgatagctc tgtatttcgg gggcgtgaca ctgctccaga
    10561 gcttgctcgg cagcctgacg ggacaagagt ctcagctagc ggtagtggtc tcgacccttg
    10621 taatagccgc cctgttcaat cctctgaggc gtcgcattca gtccttcatc gaccgtcgat
    10681 tctacaggcg cagatacgac gccagaagga ctctcgaagc gtttcacgcc cggctgagag
    10741 aggaggttga cctggatagc ctcgctgcta acctcgtctc ggtagttaaa gatacaatgc
    10801 aacctgccca cgtctcagtg tggctacgtc ccgatcctga ctcggagcga tttgatcgcg
    10861 ttgaaagcga gccaaagggt gcggagaagc aggtgagagt atcatgaagg aggccgggcg
    10921 gaaggtccgg ttcgaggaga tgtttccctg ggagatcgct cctgctatgg ccgaggcgcc
    10981 tctttgctac ctgccgctag ggacgctgga gtggcacggg gagcacgccg ccgtcgggct
    11041 cgacgcgctc aaggcccacg ccgtctgcgt cctcgcggca cagcgttcgg gcgggctcgt
    11101 cgtgccgcct ctctactggt cgaccgactg gcgtgaagac ctcactgacg gcggctacct
    11161 gaccggcggc atcgagcgcg gggagcgcta tcacgtgcca ggcaacatgt tctggctgcg
    11221 tcaggagacc ttccacaacc tgctgctcga catctacgag gcgatgcgcc gccgagggtt
    11281 cctggcgatc gtcgtcctca gcggccactg gtcgatagac cacaacatcg cggcgatccg
    11341 ggagaccggc gagaggttcc ttcgggcgca cccggctatg ggatggctcc tgctcacgga
    11401 tcaagaagtc gtgcccgacc tgcactaccc cgtagagcac gcggcaggcg gcgagacgtc
    11461 gctacttatg gcgatccgac ccgacctcgt cgccatcgac aagacgttcg agaccgaccg
    11521 ctcgctgcgt cccaactacg ccggggagcc ggatcacctg cggcgtagga gggagacccc
    11581 acacaggtac atcggggtaa atcccgcggt cgaggacgcc tccaacgacc ctgagggggc
    11641 gacgaccgaa aggggccggt tgttgctaga caccatcgtc gaacgcgtcg ccctgcgggc
    11701 gagctcgctc ctggcagaag tcacggatga cgatgaacca gcctagcgcc gcgccaatgc
    11761 gctaggcggc gaccgcggcc agcgccgcga gcccagaagg cacgcgtccg atctcatccc
    11821 agctttgccc cgtgtcctgg ctgcgcagta ccttcccgtc tccgaagcca gcgtacagcg
    11881 tccccggctc gacggggtca gggcagagtg cgtaagggaa ggcggccagg tcctccagta
    11941 gaggctccca tctctcgtca ggtgtccgcc ggtagatggc agcgcccgag aaaccgcgcc
    12001 cgtgcgcccg ccagggatcc ggggcggccg acgcgtagac gagcgagggg tcctccgcgt
    12061 cgacggcgag tccccatgcg tagtgcaggc gcataccctc gtccgcggct tcccaactat
    12121 cgccgaagtc cctgctgcgg gcgaacccgc cgccgcctga ttcgtaaagg agtcgcgggt
    12181 cttccgggtg ggcgaccagc gagtggcagt ccgcctgtgc gccggggcgc tggtcctgcc
    12241 aggtctcccc gccgtctgta ctcctgagaa cccccccgag ctcgatgccg gcgaccacaa
    12301 gatctgggtc cgtgtacgag agcgcgatgg cgcgcacgtg ggaggtccaa ggacggggag
    12361 ggaagctcca tgtgggcgcc gaggggagtt ttttcatgtc ttcaagctcc cgccaggagc
    12421 ctcctccatc acggctgacg aagagtgtcg aaggctccgt gcccgcgtag atggctccgt
    12481 ccgcgggcga gaccgcgatg gcggtgaccc tcgagtgctc tataccggaa agtttctgcc
    12541 acgtctcccc gccgtcctcg ctcttgaaga gaccttcgtc cgaagtgccg acgtagaggg
    12601 tgtcgggatc ttccggatcc accgcgacgc accgggcgcc gcgcccctct agcgatagcc
    12661 tcaccccacc gttctctata acaccaacgg cgttgttccc tgccgagtac aggcgcgaca
    12721 tggcggaccc tcctgttctc attatggttc cagatcattc ctttgggaag agtatgcgtc
    12781 atcattcacc tgtccacaag accgcagtac gtttgaggca gtaccctctg cagcgtgtag
    12841 gagcggtgtt ctcagtcgct cgacctcgtt gctactccga tgttttttct tgttcttcct
    12901 gttcctcttc tattagtgta gtcagggttt gaagcatctc aaacgtgaag cgcactatgc
    12961 cgctgtcctt gcccctgcac tcgaagtaga cagcctcgtc gtcgtagtag acgagtactt
    13021 cggagaagag ccccttgtta tagtagagtc cctcagccca ctgtgggatg gcctccttct
    13081 tcaccttgcg cgcttgctca ctcatcttct acgcctcctt ctttcttcta tgacaagttt
    13141 ctatcatgaa acagcgttac gcatactgga agggtcttgt agccgatatt cagccgagtg
    13201 tgaggaaaat tgtttgaaag agactttgcc gcccacgact ctctgtggct gcggaagccg
    13261 catccgagcg cagccacaac tcatgcgtcc gagcccctgg aaaaggtgca aaactagttc
    13321 atttagggga tacgctggct atcgtcgagg ggtagcctgt tgtgggaaag gccatcccgc
    13381 tgttgacgtg cctgagcggt accagggcgg ggaaaggaga acgccatgtc catgcaagat
    13441 cctggaagtg ctctcgactt cgaggtcctg cgcggggcta tcgaggatcg cgatgccgag
    13501 accctcctaa gcctctacgc agacgacgct gaggtgcgca cgatcaaccg caacaccacg
    13561 ccgagctctc ccctggtatt gcgcgggaag gaggagatcg gcgagtacct gcgcgacgtg
    13621 agtagccgcg agatgaccca caaggtcgag aacgaggtcg tgggaacgga caggatctcc
    13681 ttccaggagg cgtgcgagta tcccgacggg gtcagggtcc tgggagccga gaccctggac
    13741 ctgcgcaacg ggaagatcgt ccgtcaggtg aacgtcgagg cctgggacga gtagggaagc
    13801 gcctgtccga agagaacaag ccatcttcag gcgttaggtc gaggaggtcg gaaacaaggg
    13861 cagtcttgaa gtggatgacg agatcttctg aagcttcctc gcccacccgt cggaagattc
    13921 aactctcgag cgcgaccccg atgacgacaa agtggcgacc cgctggagga tgcgcgggac
    13981 tcacggaagc tcgttcaggg gtatggggac cacagtggag ctttcgggaa tagggatctt
    14041 cctcgcccag cccggcatta tgcaacagcc ctgtgccctg gcaggacaac cttacaaagg
    14101 gtgccccatc gggccggagt actcaacgat tcaggccctt ttttcctgcg tctaaacagc
    14161 gttgacggtg aatgacgcga cctgttgaac tttgccgccg gagttcaagt gagtgacata
    14221 caggatgccg gcgcttctgc taaagttggg cgattcccgg cccgttgaac aggagcatgg
    14281 attgataagt acgaacaggt atcgccgcag atgaatatac gccaggctaa cgggatcggc
    14341 agggttcacg tcgccaaacc ccagatgaag ttgacgtact gcggcaggcc gatagacgag
    14401 gaagcgtgga tcaccacctc cagggaggcc aactgcacgg gttgcgccag ggcgggcgcg
    14461 ccgacgagcc agcccagacg gtagcaaacc gggcgcgctg gggtgacttc ttagcgttcc
    14521 tggcggtgcc tcacgtcctc gacgaggtcg ctaacttcca cgaacagatc tgtcccgagg
    14581 agcttgcgta gtcctctatc ttgaagtcct gggaccaggc tcactgaagt tcttcgcacc
    14641 tctagcgtta cgaagtccca tcaggcagca ccattggctt gacgtttgcg gggtcctgtc
    14701 tgctcgcctt cagggcctcc tcggtgtcgt ccaggccgta gtggctcgtc accatcgcgt
    14761 cgaggtccac cttgcctgtc gcggcgaggt cgatggcagt ggggtacgtg ttcgcgtagc
    14821 ggaacgtgcc tgtcagtgtg atctcccttg tctggatgaa cgccagcggc acgctagtct
    14881 cttcgttcgg gcccatcccc acaacgacta cgaagccggc gggcctgacg cagcggatgc
    14941 cgtcgccaag agcgcgctcg ttgccggaac actcgatcag gacgtccgcc tccacgctgg
    15001 cctcatcaag gggttcctcg gcgacgttca tggcgcgcgt cgcgccgagg cggcgggcga
    15061 tctggagccg ctcgtcagcg acgtcggtga ccgttacttc agtggcgccc tgggcgaacg
    15121 cgacctgcat cgcgaggagc ccgatcggcc ctgctcccgt aaccaggacg tggtcgcctg
    15181 ccgttacctc tcctttcttg cacgcccaga tcccgactga cagaggctcc atgagcgcgc
    15241 ccgcgttgtc agatagctcg tctgggagtg agtatgcgaa gtcctcgtgg atgctgacgt
    15301 agttggtgaa tgcgccgtcg atggggggcg tggcgaagaa ccgcacgttc ggacacaggt
    15361 tgtagcggcc cgcccggcac tcgcggcagg tcccgtcggg gacgccaggt tcgagggtga
    15421 cgcgttctcc gacctcgtgc ttcgtcgcgc cgtctcccag agcgacgacc gtacccatcg
    15481 actcgtggcc caggatcaaa ggctccctca cgacgaacga gccgattcgt ccctcctcgt
    15541 agtaatgaac gtcggaaccg cagacaccga cggccctgat ctcgaccagg acctcctttg
    15601 ggccaggttc aggaacggga acctcttcga gcctgacgtc gtgggtcccg tacatgaccg
    15661 ctgctctgtt gtgcatgagg ttcctttctc ttactccccc gtgaccgcgc cgagggtgag
    15721 gcctcttacc aggtagcgcc tcacggccag cccgacgatt atcatgggga gcacgactat
    15781 cgtgcctatg gctgagagct ggccccagct caggcccgtc tgcgtgttca gctgcgctgc
    15841 ggcgataggg agggtctggc tgttcggtcc ggcgaaggtt gcggctaagg cgtagtcgtt
    15901 ccacgagaag accaggcaga ggatggccgt cgtcactatg ccgggc
//
LOCUS       DATAIR010000234         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_52872, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000234 DATAIR010000000
VERSION     DATAIR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52872"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHM69_05660"
     CDS             <1..333
                     /locus_tag="VHM69_05660"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739914.1"
                     /translation="AYEIQVESLPGDIPQEITIDVSGLGMHENLHLGDLTLPEGVTLL
                     SEAEGVAVTVTSPTEITEEEMEAAGIVEEPTEEEEAAAAAEEAAEGEEAPEEEAPEEG
                     TADQDEQA"
     gene            320..919
                     /gene="pth"
                     /locus_tag="VHM69_05665"
     CDS             320..919
                     /gene="pth"
                     /locus_tag="VHM69_05665"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX2739915.1"
                     /translation="MNRLRLVRSFFKREERDDGGPTRSPVVVGLGNPGRSYERTRHNA
                     GYLVADELAKRHGGSWRRKKRSEAAPVSLGLKNITILKPTTFMNNSGSAVSSYSPEDL
                     IVVHDDLDLEAGTVRVKVGGGAGGHNGLRSIIQSLGNDFVRVRIGIGRQPAGVTVTDY
                     VLSRMDSVVKDAIPRAADAVEFVIEEGPEAAMNRFNVRA"
     gene            complement(943..>1548)
                     /locus_tag="VHM69_05670"
     CDS             complement(943..>1548)
                     /locus_tag="VHM69_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2739916.1"
                     /translation="RMILGVGIGAMPYEWDYLGEEADMRVRGAMLDEGLEVLTGLWTG
                     EPFAHRGEHYSIHGESPDEDWRAVFYPPPLQRPRVPIWVAGTWPVKPPFRRAARWDGV
                     FPMKAEGRRILPMTPEDVRDVTRYVAEHRTEEGPFDVVVAGETRGDDRATGADIVEPY
                     EEAGLTWWIESIDPWRFGWTDNRPWPTGRMRERVRQGPPES"
BASE COUNT          287 a          510 c          496 g          255 t
ORIGIN      
        1 gcatacgaga ttcaggtcga gtcgctccct ggcgacatcc cgcaggagat caccatcgac
       61 gtctcgggtc tcggtatgca cgagaacctc cacctcggcg acctcaccct tcccgagggc
      121 gtgactcttc tctccgaggc cgaaggggtc gcggtcacgg tcacgtcgcc gacggagatc
      181 accgaggagg agatggaggc cgcaggcatc gtcgaggagc cgacggaaga ggaagaggct
      241 gccgctgcag cggaagaggc tgccgagggt gaagaagctc ctgaggaaga agctcccgag
      301 gagggcaccg cagaccagga tgaacaggct taggctggtc cgctctttct tcaagcgaga
      361 ggaacgagac gacggggggc cgacaaggtc ccccgtcgtc gtcggcctcg gaaaccctgg
      421 ccgctcatac gagcgcaccc gccacaacgc cggctacctg gtcgcggacg agctcgcgaa
      481 acgccacggc ggctcctggc gcaggaagaa gagatcggaa gccgcacccg tctcgctcgg
      541 cttgaagaac ataactatcc tgaagccgac gacgttcatg aacaactcgg gctccgccgt
      601 atcgagctac agtcctgagg acctgatcgt ggtccacgac gacctcgacc tggaagcggg
      661 tacggtgcgc gtgaaggtcg gaggcggcgc aggcggccac aacggcctgc gctcgatcat
      721 ccagagcctc ggcaacgatt tcgtccgggt ccgtatcggc atcgggcgtc agcccgccgg
      781 cgtcaccgtc acggactacg tgctctctcg tatggactcg gtcgtgaaag atgccatccc
      841 cagagccgcc gacgcggtgg agttcgtgat cgaggagggc ccagaggcgg ctatgaaccg
      901 ctttaacgtc cgtgcctgac aatccgtgac cggccgtgcc tcctacgatt cgggcggacc
      961 ttgtcgcacg cgctcgcgca tccttccggt cggccagggc cggttgtccg tccatccgaa
     1021 gcgccaggga tctatgctct ctatccacca ggtgaggcca gcttcctcgt aaggttcgac
     1081 gatgtcagca ccagttgccc ggtcgtcgcc gcgggtctct ccggcgacga cgacgtcgaa
     1141 aggcccttct tcggtacgat gctccgctac gtagcgtgtc acgtcgcgta cgtcctcggg
     1201 ggtcatcggg agtatcctcc ggccttcggc cttcatcggg aaaacgccgt cccagcgcgc
     1261 ggcgcgtcgg aacggaggct tgacgggcca ggtaccggcg acccagatcg ggacgcgggg
     1321 gcgttgaagg ggcggcggat agaacaccgc ccgccagtct tcatcaggac tctctccatg
     1381 gatactatag tgctcgccgc ggtgtgcgaa gggctcgcct gtccacagtc ccgttagcac
     1441 ctcgagtccc tcatcgagca tggcgccgcg gacccgcata tccgcctcct cacccagata
     1501 gtcccactcg tagggcatag cgccgatccc gacgcccagt atcatgcg
//
LOCUS       DATAIR010000235         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_237610, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000235 DATAIR010000000
VERSION     DATAIR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237610"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VHM69_05675"
     CDS             <1..443
                     /locus_tag="VHM69_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739917.1"
                     /translation="EDRGGLGLAALWAHTLPDLIYTALKERSTMLARNTYRSVVGVAL
                     AAAFLLVWINLAVGIIGHSGDLANSMYVGVLAVGIIGAIIATALAQALVAVIVLIFGL
                     GLPWSPPVEILGLTGFFVALFVGSALLFQFAARVQPPAGAGQEG"
     gene            628..1401
                     /locus_tag="VHM69_05680"
     CDS             628..1401
                     /locus_tag="VHM69_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2739918.1"
                     /translation="MYFEDDGGDGEAVVLHGGLVDSVELVRASNIAQALQELPEEFRL
                     IYVDHRGVGRSDKPHEVEAYAMPLRVADAVAVLDKLNIQRAHFIGNSWGGRLGFGIGE
                     HAPERVLSLVIGGQQPYAIRLDGPLAHAVTETLVESRRKGSLEPFVDILESFAGGRLP
                     DAMRERWLDNDPVAIEAAWSAGLAEGEISEDLRGWQIPCLIYAGSGDVDFQDQARRAA
                     NEIPNAEFIALDELDHVSAHLAQVDPLLPAVVRTLRRNG"
     gene            1865..>2387
                     /locus_tag="VHM69_05685"
     CDS             1865..>2387
                     /locus_tag="VHM69_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2739919.1"
                     /translation="MLLVLVLLSCLVVAAIFLWYRERPAETVTLTALDRPASEEPPEP
                     PVPPARVHAVEELDEEFRAISEAHTGTHGVVVFDLYSGKSASLNADRRFVAASLSKLY
                     ALLTLYRAASRGELSLNDEITMRASDVWAEGTGVLYRYPVGHTMTLRECATFLIKESD
                     NTAEVMLNRYLGEE"
BASE COUNT          455 a          699 c          755 g          478 t
ORIGIN      
        1 tggaggaccg cggcggcttg ggtctggcgg cgctgtgggc ccacaccctg ccggatttaa
       61 tctataccgc gctcaaggag aggagcacta tgttggccag gaacacgtac cgatccgtcg
      121 ttggcgttgc gctcgcggcc gcgttcctcc tcgtctggat aaaccttgcc gttggcataa
      181 tcgggcattc gggcgacctc gccaactcga tgtatgtcgg agtgctcgcc gtcggaatca
      241 tcggtgccat catcgcgacg gcgctcgccc aggcgttggt cgccgtgatc gtgctgatct
      301 tcgggttggg tctcccgtgg agcccgccgg tagagatcct gggcctgact gggtttttcg
      361 tcgcactgtt cgtcggatcg gcgttgttgt ttcagtttgc tgcgcgggtg caacctcccg
      421 cgggcgcagg gcaggagggt tgacccgcag ctacgtccga gagaatcagg catagccgcc
      481 gcttttcacc ggagtgcgta gaagtatgga ttccttgaaa gtagcataac cttcgcactc
      541 ttcgatacaa taggtgcaat acgctccggt agggagcggg gaaggacact catgccttac
      601 gctggcaacg cgctggatgg aaacctagtg tactttgagg atgacggcgg cgacggagag
      661 gcggtcgtcc tccacggagg gctagttgat tcggttgagc tggtgcgagc ctcgaacatc
      721 gcccaggcgt tgcaggaact gcctgaggag ttccgcctca tctacgtcga tcaccgcggt
      781 gtcggacgca gcgacaagcc ccacgaggtg gaggcatatg cgatgccgtt gcgtgtggct
      841 gatgcggtgg cggtcttaga taaactcaac atccagcgcg cacacttcat cggcaactca
      901 tggggaggga ggctcggctt cggcatcggc gaacatgccc cagagcgggt gctctcgctc
      961 gtgatcgggg gccagcagcc gtacgccatc cgcctcgatg gaccgctcgc tcacgcagtc
     1021 actgagactc tggtcgaatc gcgaaggaag ggtagtttgg agcctttcgt cgatattctg
     1081 gagtcgtttg ctggtggccg gttgccagac gctatgcggg agcggtggct cgacaatgac
     1141 cctgtggcta tcgaagcggc atggagcgca ggtctggctg aaggggagat ctccgaagac
     1201 cttcgggggt ggcagatccc atgcctcatc tacgcaggca gtggggatgt cgacttccaa
     1261 gaccaggcgc ggcgtgcggc aaacgagatc ccgaacgcag agttcatcgc gcttgatgaa
     1321 cttgatcacg taagcgcaca cctggctcag gtcgatccgc tgctccccgc cgtcgtgcgc
     1381 acattgcgca ggaatggctg atagctcctc ctcgtaactt ctgaggggaa ctgagtacta
     1441 gaaggatgga attctcagca cgcattgacg actacccatg tactccaacc gggctgatac
     1501 gcactagtac agaatggaca aagcgactcc gccagatcgt cgcaaaccgc ctgaacaagc
     1561 gcaatttcca gactcagaag gtcaggctag aatgtgttca agcaaactcg taatgagcag
     1621 taaagcggtt cgagcacgct cttcggctct cactattccg cttagataag ctggactttc
     1681 ctggcaatcc agggccccat tcctgcgccg gggccttttg accctaatgg tgctgtaaag
     1741 tgcgtgtcta gatatggccg gacgcgagag acgaccttat ggacatattc gccgagcatg
     1801 gggacaggct cctggggtcc ctgcgggggg ccgaagtagg cggacgccgc gacgcagtct
     1861 gtgggtgctg ctggtcctag ttctcctctc ctgcctggtc gtggccgcga tcttcctgtg
     1921 gtaccgcgag agaccggccg agactgtgac tctgacggcc ctggacaggc cggcctcgga
     1981 ggagccacca gaacctcccg tgcccccggc gcgagttcac gccgtcgaag agctcgacga
     2041 agaattccga gccatctccg aggcgcacac gggtactcac ggggtagtgg tctttgatct
     2101 ctactctgga aagagcgcct cgctcaacgc cgaccgacgc ttcgtggccg ccagcctcag
     2161 caaactgtac gccctgctta cgctctacag ggccgcctcc cgaggcgagt tgagcctgaa
     2221 cgacgagatc accatgcgcg cctcggacgt ctgggcagag ggcacgggcg tgctctaccg
     2281 ctaccctgtc ggacacacca tgaccctgcg cgagtgcgcc accttcttga tcaaggagag
     2341 cgacaatacc gcggaggtca tgctcaaccg ctacctgggt gaggagg
//
LOCUS       DATAIR010000236         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_52899, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000236 DATAIR010000000
VERSION     DATAIR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52899"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VHM69_05690"
     CDS             <1..583
                     /locus_tag="VHM69_05690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2739920.1"
                     /translation="TEAWARAFERVGHRVPRFRLHKQIGKGAELLISEFVEDEETLHK
                     VQDLHSEIYEELQEYGHPLPGAKELISSLKQRNYGVWFVTSAKDEELEHHMQELEAED
                     IIDGVVNSSAVENSKPASDIFNETLKRAGATADETVAVGDAVWDVEAAREAGIRTVAV
                     LSGGAYDKEALEEAGAVAVYEDCAALLDSGFPE"
     gene            716..919
                     /locus_tag="VHM69_05695"
     CDS             716..919
                     /locus_tag="VHM69_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2739921.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHFTGIEGTGFKTLEE
                     GAKVAFETSQGKKGLQAVNVTPA"
     gene            complement(1090..2289)
                     /gene="larC"
                     /locus_tag="VHM69_05700"
     CDS             complement(1090..2289)
                     /gene="larC"
                     /locus_tag="VHM69_05700"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX2739922.1"
                     /translation="MTHVHFQPVGGAAGDMTLAALVAAGAPLPEITASLRGLGVDFGL
                     STERVEVNGVGALQANVEHPVEHSHRTFRDIRALIQRASLPDRASSRALEAFRRLAVA
                     EGAVHDENPEEVTFHEVGAVDSIVDVVGSCVALELLDAASVSCGPLPMGTGVVDAAHG
                     SIPVPGPATLEILKGSRLRWTDEPRETTTPTGAALMRSFTGGEFTDAAPPMTLRATGY
                     GAGRAHLRSAPNLLRAVVGELEGQVGDVELIEANIDDAPGEFLGAAVERLLAAGALDA
                     WLEPIVMKRGRGAYKACALALSSDRERLARLLMRETGTLGVRHHAVGRTVAERRRVLV
                     ELPYGKCRIKVGSIDGEDFVVSPEYADAVHLATETGLPLPKVYEDANEGYRHQATGIS
                     KNQVSGT"
     gene            complement(2311..2658)
                     /locus_tag="VHM69_05705"
     CDS             complement(2311..2658)
                     /locus_tag="VHM69_05705"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2739923.1"
                     /translation="MSEIPERIEREMFEIRSRMAPDVRDLKKHTEPQVIVKQTSKKVK
                     GRVRDAVIRFGKSLKESASRQARMVGEAGRKRNPAPLTDAVKRDPRPLILLAISLAVT
                     MMAVRKISNGRGE"
     gene            complement(2658..3095)
                     /locus_tag="VHM69_05710"
     CDS             complement(2658..3095)
                     /locus_tag="VHM69_05710"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2739924.1"
                     /translation="MDGRDRVAEFGRELREGPEPSDRSIKEIIDALRPQLQELASKQV
                     ELAKLEMAPVGKRAGLATGLLVAAGVFMFVFLIFLSLTGVYLLAIFFPLWVSALIVSG
                     ILLLVGGILAGAGASILRRLDPKPHRTIHTLQQNINWIKGQLR"
     gene            3313..4059
                     /gene="yqeC"
                     /locus_tag="VHM69_05715"
     CDS             3313..4059
                     /gene="yqeC"
                     /locus_tag="VHM69_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HEX2739925.1"
                     /translation="MKLHVALGISSGDVVAFVGAGGKSSAIVAASGELTQAGMKVIVA
                     PTTKMLLSEAKSIGPLVTSEDAGELRAKTENALSGAPGVVVGSGMLSKNRVGGVDPEW
                     VGDLAPLADVVLIEADGSRRRPIKGTASHEPAIPEAATLVVAVANVHALGTPVDEEHV
                     HRPDVFSNLTGVEQGQSITPRAFALALAKGSLAHVPQGARIAVLITGVEPGRSMSDAS
                     VITRELWRLGAKNVVLTSLPNESPAHVWVP"
     gene            4156..>4436
                     /locus_tag="VHM69_05720"
     CDS             4156..>4436
                     /locus_tag="VHM69_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2739926.1"
                     /translation="MRVVAVWIDVDPRSGGPIYVQIVDQVRHAIEVGGFGPGERLPTV
                     RRLAGDLAIAPNTIVKAYNELQRMGLIESRPGVGTVVADGVAEVARERQV"
BASE COUNT          850 a         1370 c         1409 g          807 t
ORIGIN      
        1 cacggaggcg tgggcgcggg cgttcgagag ggtgggccat cgggtgccga ggttcaggct
       61 ccacaagcag attggtaagg gcgcagaact tttgatcagc gagttcgtcg aggacgagga
      121 gaccctgcac aaggtccagg acctccacag cgagatctac gaggaactgc aggagtacgg
      181 acacccgctt ccgggggcga aggagttgat ctcctctctc aaacagcgga actacggggt
      241 ctggttcgtg accagcgcaa aggacgaaga actggaacat cacatgcagg agctcgaagc
      301 ggaggacatt atagacggcg tcgtcaactc cagtgccgtc gagaactcca agccagcctc
      361 cgacattttc aatgagaccc tcaaaagagc cggagccacg gcggacgaaa ccgtcgccgt
      421 cggtgacgcg gtctgggacg tcgaggcggc cagggaagcc ggtatacgca ccgtcgccgt
      481 cctctccggc ggcgcctacg ataaggaggc cctcgaagag gccggagcag tggccgtcta
      541 cgaagactgc gccgcgctcc tggattccgg cttccctgaa tagcgcccgc cttgcgtgaa
      601 aggaacgggg ttttgtacta gagtgctact cgcaagctgg aacagggttt gatctgggac
      661 gtcaaataat gatctctcgg tctggcccgg gaaaagaaag gtgggcgcaa acggcatggc
      721 ccagggaacg gtaaagtggt tcagcgacga gaagggctac ggcttcatct ccccggacga
      781 cgggggtgaa gacctcttcg ttcacttcac gggaatagag gggaccgggt tcaagaccct
      841 agaagaaggc gccaaggtag ccttcgagac gtcccagggg aagaagggac tacaggcggt
      901 aaacgtaaca ccagcctgag aacaaaaagg gatgatgctt ggagggtcgg cggggctccg
      961 ccggcccctt tttgtaggtt tcaggctata ggcatcaggc ttcagctctt gcaggaggat
     1021 gcccgagcct tcgacgaagc atggagtggg ggataccggt actttcgggg tgtgccgcga
     1081 tagcgggtat caggtaccgg aaacttgatt cttgctgatg cctgtagcct gatgcctgta
     1141 gccttcattt gcatcctcgt acactttggg cagagggagc ccggtctccg tggcgaggtg
     1201 gacggcgtca gcgtactcgg gggagacgac gaagtcctca ccgtcgatgc tgccgacttt
     1261 gatcctgcat tttccgtaag gcagctccac gaggacgcgg cgtcgttcgg ctacggtgcg
     1321 tccgacagcg tggtgacgca cgccgagggt gccggtctcc cgcatgagta gtctggcgag
     1381 acgctcgcgg tcgcttgaga gggccagagc acaagctttg taagcaccgc gaccgcgctt
     1441 cattactatg ggctcgagcc aggcgtcgag ggcgccggcc gcgaggaggc gctccaccgc
     1501 cgccccgagg aactcaccgg gggcgtcgtc tatgtttgcc tctattagct cgacgtcgcc
     1561 cacctggcct tcgagctccc cgacgacggc gcgcagcagg ttcggtgcgc tccgcagatg
     1621 cgcccgcccg gccccgtagc ccgtggcgcg cagggtcatg ggcggggccg cgtcagtaaa
     1681 ctcgccgcct gtgaaggatc tcatgagcgc ggcgccggtt ggtgtggtcg tctcgcgggg
     1741 ctcgtctgtc cagcgtagtc tcgaaccctt gaggatctcc agcgtggccg gtccgggcac
     1801 ggggatggac ccgtgggcag catcgactac gcccgtgccc atcggcaagg gaccgcagct
     1861 cacggaggcc gcgtcgagca gctccagcgc cacgcagctc ccgaccacgt cgactatgga
     1921 gtccaccgcc ccgacctcgt ggaaagtgac ttcttcgggg ttctcgtcgt ggacggcgcc
     1981 ttccgcgaca gccaggcgac ggaacgcctc gagtgcacgc gaggaagccc ggtcaggcag
     2041 acttgccctc tgtataaggg cgcggatatc gcggaaggtg cgatgggagt gctcgacggg
     2101 gtgttcgacg tttgcctgga gcgcgccaac gccgttcacc tcgacccgct cagtgctcag
     2161 gccgaagtcc acgccgaggc cgcggaggga ggctgtgatc tcaggtagtg gcgctccagc
     2221 agcgacgagc gccgcgaggg tcatgtcgcc ggctgctccg ccgacgggtt ggaaatggac
     2281 gtgggtcaaa ccaggactga gggtactacc tcattcccct cgtccgttgg agatcttgcg
     2341 caccgccatc atggtgacgg cgaggctgat ggcgagcagg atcaggggcc ttggatccct
     2401 cttcacggcg tccgtgagcg gtgcagggtt cctcttcctg cctgcctccc cgaccatcct
     2461 ggcctggcgg ctggcggatt ccttgaggct cttgccgaag cggatcacgg cgtctctgac
     2521 gcgccccttg accttcttag aggtctgctt gacgatgacc tggggctcgg tatgcttctt
     2581 gaggtcccgg acgtccggcg ccatgcggct ccggatctcg aacatctccc tctctatgcg
     2641 ctcaggtatc tcgctcatta cctcaactgc ccctttatcc agttgatgtt ctgctgaagg
     2701 gtatggatcg tacggtgggg cttgggatcg agcctcctca gtatgctggc accggctccg
     2761 gcaagtatcc ccccgacgag cagcagtata ccactgacga tcaaagccga gacccacaac
     2821 gggaagaaga tagccagcag atatacgccg gtgagagaga ggaaaatgag gaagacgaac
     2881 atgaagacgc cggcggccac gagaagcccc gtcgccagcc ctgccctctt gcctacgggg
     2941 gccatctcca gcttggcaag ctcgacctgc ttgctggcga gctcctgaag ctgcggcctc
     3001 agagcgtcta ttatctcctt gatgctcctg tcagaaggct cgggaccctc ccttaattcc
     3061 ctgccgaact ccgctacccg atcccgcccg tccatcctca cgctcctcgc atctgttccg
     3121 aaaaccacta gtctttgtta gttaccctcg ccccgccccg cgtaatccga caggtcccgc
     3181 aggtacgacc atccctatcc tacactacat cctagtatcc aacatgccat aaagcacgcg
     3241 aagtgacggg ttgatgtaac atgaatacag ttgtacaagg cccgccgctt cctggcgggg
     3301 aggggagtgg gtttgaagct gcacgtggcg ctaggtatct cctcgggaga tgtggtcgcc
     3361 ttcgtcggtg ccggaggtaa gtccagtgcg atcgtcgccg cctcgggcga gctcacgcag
     3421 gctggcatga aagtcatcgt ggccccgacg acgaagatgt tactgagcga ggccaaaagc
     3481 ataggcccgc tcgtcacctc cgaagacgcc ggcgaactgc gcgcgaagac cgaaaacgct
     3541 ctctccggag cacccggggt cgtagtcggc tcggggatgc tctcaaagaa cagggtgggc
     3601 ggtgtggacc ccgaatgggt cggagatctc gccccacttg ccgacgtggt cctcatcgag
     3661 gcggatgggt cgcgccggcg cccgatcaaa ggcacggcca gccacgaacc agccatcccc
     3721 gaagccgcca ccctcgtcgt cgcagtcgcc aacgtccacg ccctcggtac gcccgtggac
     3781 gaagaacacg tccaccgccc cgatgtcttc tccaacctca ccggcgtcga acaaggccag
     3841 agcattaccc caagggcctt cgccctggcc ctcgcaaagg gctcgctcgc ccatgtcccg
     3901 caaggggcca ggatcgccgt cctcatcaca ggcgtcgaac caggccgctc tatgtccgac
     3961 gcctccgtta tcacccgcga actctggcgc ctcggcgcca agaacgtcgt ccttacctcc
     4021 ctacccaacg aatctccagc ccacgtctgg gtcccgtaaa actctcccgc caaacgtcct
     4081 tctaaaccct gactccacac ttatagacac gctctgtgtc attgtgttat aaatatgcga
     4141 cattaataca gggaggtgag agtcgtagct gtctggatcg acgttgaccc gcgtagtggc
     4201 ggtccgatct acgtgcagat cgtggatcag gtaaggcatg cgatcgaggt cggaggtttc
     4261 gggcctgggg agcgcttgcc gacggtacgc cggctcgccg gggacctcgc tatagcgccg
     4321 aacacgatcg tcaaggcgta caacgagctg caacgtatgg gactcatcga gagccggcct
     4381 ggcgtgggga cggtcgtggc tgatggggtt gcggaggtgg cgcgagagcg gcaggt
//
LOCUS       DATAIR010000237         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_430030, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000237 DATAIR010000000
VERSION     DATAIR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430030"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VHM69_05725"
     CDS             complement(<1..1175)
                     /locus_tag="VHM69_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2739927.1"
                     /translation="MATSEADVNRQHDDDSRVTGQQWKWTVLAGMASYLDAGSIVALA
                     AGLALWQEYLGMSSGTVGLLAALGPNAFGAAIGALIGGRLGDLFGRKRIYQYDLLVYA
                     LGILLIVFSVNLPMLMIGTLIVGVAVGADVPTSLALIGEFSPSKARGKLMGLSQVAWS
                     LGPIVVFVLAFALSSYGVLGSRIVFAHLFVVAIVTWALRRGMVESAIWTAASGAGETA
                     PTTDPEAQPEEAGVPADPLAASRLRGLFSGPTLAAILFTLIVYLFWNLAAGTLGVFFP
                     YILKTLGAQSQAASVALSCAGFVITLLAVVLIFMPFSDRSDRARRIMWGVGAIMNVVA
                     FAQLLIFPFNTVTAITMIVLFGIGAAIAGEPFYKTWSQELFPTMLRTTAQGLTFGVA"
     gene            complement(1251..2036)
                     /locus_tag="VHM69_05730"
     CDS             complement(1251..2036)
                     /locus_tag="VHM69_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX2739928.1"
                     /translation="MSEKRADLPEQRQQRIAMLLRASGSVTVAGLEAELGISAATARR
                     DLAVLERQGKVKRTHGGAVLPGVAQHEDSFQQRLGEAVEAKKRLARAASALLEAEETV
                     FIDSSTTAYYATQRMLAESPSVTYLTNLVPGMELFSVADPSGTSMVGLGGIFRPLTLS
                     FVGPCTIRMIESYLADRAFFSVKGITPEGHLTEPNPLEAEVKRAMIRRADNPVLLVDG
                     RKFERRGASVIAHVSEVSLVITTEARRSYVEALEDLGVEVQSI"
     gene            complement(2263..>3229)
                     /locus_tag="VHM69_05735"
     CDS             complement(2263..>3229)
                     /locus_tag="VHM69_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2739929.1"
                     /translation="SGQVLVKMKASGLCGSDLRAIYHEHTGSGAERYQNVIAGHEPAG
                     QVEAVGPGVKGFEPGDRVVVYHILGCGRCEECLKGFMIGCRSPERAAYGWQRDGGHAD
                     YLLAEARTLLHLPDELTYTDGALVACGFGTAYQGILRAGVSGRDRVLVVGLGPVGLGA
                     VMLAASSGAEVIGVDLIPERLELAKRAGAAHVLMGGESTTEEVLDLTDGHGTEVGIDC
                     SGSASGRKMCLEAAREWGRVVYIGEGGSVTFEPSPLLLHKQLTLHGSWVCGINEMGEL
                     LEHLARKELHPEATVTHTFPLSDTKQAYETFDAGRTGKVVILWQD"
BASE COUNT          607 a         1084 c          964 g          574 t
ORIGIN      
        1 ctggcaacac cgaacgtgag tccctgcgcc gtggtcctga gcatggtggg gaacagctcc
       61 tgcgaccacg tcttgtagaa tggttctccc gcgatcgctg ccccgatgcc gaagaggacg
      121 atcatggtga tggcggtcac agtgttgaag ggaaagatga gcagctgggc aaacgcgacc
      181 acgttcatga tggcaccgac gccccacatg atgcggcggg cgcggtcgct gcggtcgctg
      241 aagggcatga agatcaggac gacggccaac agtgtgatga cgaagcctgc gcacgacagg
      301 gcgacgctgg ccgcctgact ctgtgcaccg agggtcttca ggatgtaagg aaagaagaca
      361 ccgagggtcc ccgcggccag gttccagaac aggtacacga tcagggtgaa caagatggct
      421 gccaaggtcg gcccggagaa cagcccgcgc agacggctcg cagccagcgg gtcggcaggc
      481 acgcccgcct cctcgggctg cgcttccggg tcggtcgtgg gcgccgtctc gcctgcgcct
      541 gaggcggctg tccaaatagc agactccacc atgccccgcc tgagagccca cgtaactatc
      601 gcgaccacga acaggtgggc gaagactatc ctgctcccta gcacgccgta cgacgagagc
      661 gcgaatgcga gcacaaatac gactatcggg ccgaggctcc aggccacctg gctgaggccc
      721 atcagcttgc cgcgggcctt gctaggcgag aactcgccga tcagcgcgag cgaagtaggc
      781 acgtcggcgc cgacagcgac accaactatt aaggttccga tcatgagcat cggcaggttg
      841 actgaaaaga cgatcagcaa gatgcccaag gcgtatacga gcaggtcgta ctggtagatg
      901 cgcttgcgcc cgaacaggtc gccgagccgc cccccgatca gggccccgat cgccgccccg
      961 aaggcgttag ggcccagggc tgcaaggagt cctacggtgc cgctgctcat acccaggtac
     1021 tcctgccaca gcgcgaggcc cgcggcgagg gcgacgatgg agccagcgtc gaggtaggaa
     1081 gccatgccag ccagtacggt ccatttccat tgctggcccg tgaccctgct gtcgtcgtcg
     1141 tgctgtctgt ttacatccgc ttccgaagta gccatggtct agataaagcc tccccctgcg
     1201 cccttccaca ttcccatcta ccgacgtaac gcgtgatcag gtccctcgtg tcatatgctc
     1261 tgcacttcca cccccaggtc ctcgagagct tccacgtacg agcggcgggc ttctgtggtt
     1321 atcacgaggg acacctccga gacgtgggct atcacgctcg ccccacgccg ctcgaacttc
     1381 ctgccgtcca ccaaaagcac ggggttgtcc gcgcgccgga tcatggcgcg cttgacctcg
     1441 gcctccagcg gatttggctc cgtgagatgc ccttcgggtg tgatcccctt gacgctgaag
     1501 aaggccctgt ccgcgaggta agactcgatc atgcggattg tgcatggccc cacgaatgag
     1561 agcgttagcg gcctgaagat cccaccaagc ccgaccatgc tcgtgccgga cggatcggcc
     1621 acagagaata actccattcc aggcaccagg tttgtcagat aggtgacgct cggagactcg
     1681 gcgagcatgc gctgagtcgc atagtacgcc gtggtcgacg agtcgataaa gacggtctcc
     1741 tccgcctcca acaacgccga tgccgcacgc gccagtcgct tcttcgcctc caccgcctcg
     1801 cctaaccgct gctggaagga gtcctcgtgc tgcgccaccc caggcaagac agccccacca
     1861 tgagtcctct tgaccttccc ctgacgttcc agcaccgcaa gatcccgcct cgcagtcgcc
     1921 gccgaaatgc caagctccgc ttctagcccg gcgaccgtga cactccccga tgcacgaagg
     1981 agcatcgcta tccgttgctg acgctgctcc ggtagatctg cgcgtttttc cgacatattg
     2041 tgattgaacg agcagttttt cttctaaacc gctcaaatcc ttccctcgcc cgctcatact
     2101 gtaacggagg ctgcatttcg ctgcaagcct gtggtggctt tacgctggct tcgggatctg
     2161 ccaacgcagg gtgagggttg gggtcgagga ctccccaggg acggccacga ttctctcggt
     2221 cactggcgga gatgcttcag ttgggagcgt gagccgtgca agtcagtcct gccagaggat
     2281 cacgactttg cccgtcctac cggcgtcgaa ggtctcgtag gcctgtttgg tgtcggagag
     2341 ggggaaggta tgggtcacgg tcgcctcggg gtgcaattct ttgcgggcaa gatgttcgag
     2401 gagttccccc atctcgttta tgccgcagac ccatgagccg tggagcgtga gctgtttgtg
     2461 caacagcagc gggctcggct cgaaggtgac ggaaccgccc tctccgatat agaccacacg
     2521 cccccactcg cgggccgcct ccaggcacat cttcctccct gaggcgctcc ccgaacagtc
     2581 gatgccgacc tcagtgccgt ggccatcggt gaggtccagc acctcctcag tcgtgctctc
     2641 tccgcccata aggacgtgcg cggcgcccgc ccgcttcgcc agctcaagtc gctcggggat
     2701 caggtccacc cctattacct cggcaccgct cgatgcggct agcattaccg cccccagtcc
     2761 gacaggtccg agcccgacga cgaggaccct gtcccggccc gagaccccgg cgcgcaatat
     2821 cccctgatac gcggtaccga acccgcaggc cacgagggcg ccgtctgtgt aggtaagctc
     2881 gtcaggcagg tgaagcaggg tgcgcgcctc ggccagtaaa tagtcggcgt ggcctccgtc
     2941 gcgctgccat ccataagccg cccgttccgg agaacgacac ccgatcatga accccttcag
     3001 acactcctca cagcgcccgc agccaaggat gtggtagacg acgaccctgt cgccgggctc
     3061 gaatcctttc acaccgggtc ccacggcctc gacctgcccg gccggctcgt ggcccgcgat
     3121 gacgttctgg tagcgttccg cccccgagcc cgtgtgctcg tgatagatgg cgcgcaggtc
     3181 gctcccgcag agccccgacg ccttcatctt gacaagtacc tgaccagat
//
LOCUS       DATAIR010000238         4384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_145580, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000238 DATAIR010000000
VERSION     DATAIR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145580"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHM69_05740"
     CDS             <1..657
                     /locus_tag="VHM69_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2739930.1"
                     /translation="APGILEILARNGIRDGLVVDLGCGSGQWARELVDAGYRVLGIDI
                     SEDMIELSRRRVPEAEFRVGSLFDADIPPCGAVTAISEVLNYLFDEEAGGLRPLFGRV
                     FEALAPGGVFVFDVLGPGQVPPGTTAKGFRVGEDWAVLSEREEDSGRGTMTRRIVSFR
                     KVGEHYRRDDEVHRVRLYDPSELSAELGEAGFRVNTVRAYGDYPLGENHVAFEARKPV
                     "
     gene            703..1563
                     /gene="mutM"
                     /locus_tag="VHM69_05745"
     CDS             703..1563
                     /gene="mutM"
                     /locus_tag="VHM69_05745"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2739931.1"
                     /translation="MPELPEVETIKGDLRELVVGSNVERAEVLDSSLVEQPSTEEFIN
                     RLDGVRITGARRRAKHLIVELDSGDSLVFQLKIGGQLLLVPPVEEPNTALMLVLHLDG
                     ERRLFLRDETGFTRARLLDAGELEERLSPLGPEPLEEGFDEGYLQRVIGSRRAQIKPL
                     LLDQKVVSGIGNIYADEILYDARLHPRRKANTLSEGEWVALHAAIRTNLAAGVEHRGT
                     TVRLYRDVLDRPGEHQNYLRVFEKHGRPCPGCEGEVVREKVGGRPSHFCPECQREDGP
                     EEEGTLESLW"
     gene            1582..2997
                     /gene="lpdA"
                     /locus_tag="VHM69_05750"
     CDS             1582..2997
                     /gene="lpdA"
                     /locus_tag="VHM69_05750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX2739932.1"
                     /translation="MADKFDLVVIGGGNGGYIPAIRASQLGMKVALIDKREGGHLGGT
                     CLNVGCIPTKALLQTAHLLHDAKNGADFGVKISDVEFDYPQAARRREQVVNQLRKGVQ
                     GLMKKNKVTVYNGVGSFVEPKKIKVETADGEEELEADHVLISTGSTVSTLPGLEFDGE
                     KVISSDDIVTNDESYPKSVIVLGSGAVGVEFASMYHDFGVEVTVVELLDRIVPLEDPE
                     ISAALEKEFEDRGMRVLTGTKADPESLEKTDSGVKITVAGGDDGEETLEAEALLVAVG
                     RRTVTEDLNLDVTKVELDDRGIIQVDGYGRTGEDGIYAAGDVIGGYWLAHAAGHEGII
                     AVEHMAGENPMPMNQDLVPRVTFCRPEVASFGLSKAQAEEEGYEVKESKFPFRAIGKA
                     LIEGEPNGFFKIVADAETDLILGMHAIGPKVTDLITEGVFAKLVEGTPEELAMTIHPH
                     PSLAEIVGESAMAVEGHPIHF"
     gene            3191..3889
                     /locus_tag="VHM69_05755"
     CDS             3191..3889
                     /locus_tag="VHM69_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739933.1"
                     /translation="MTSDVVTGLHNPGELAFDPVRDGFGFRNPVGEVAIGNGRGPLLR
                     RLGAILYGKGLCFGMVTMALRRFAEQPADASERPLAGLPPTPELLGEIRRYHVRQYMP
                     RAVLAAVKDWLRSRGGKPDSVLRRLRLAGTDSHLICFGPAVNRRFLWCLMRAHAVAPY
                     RVETENREARVYVYDPNYPGDRGRYVNFRRDGSGRAREFEYEGFGTRWGWGISLFPHS
                     AIGDTARHGLRAIP"
     gene            4040..>4384
                     /locus_tag="VHM69_05760"
     CDS             4040..>4384
                     /locus_tag="VHM69_05760"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2739934.1"
                     /translation="MPFRQQSRGVPGAHGRPEWLKARIPDGEVYRDIKETMRGLNLHT
                     VCEEARCPNIGECWNNRTATFMILGNVCTRSCGFCAVLTGKPTELDLDEPHRVADAAR
                     QMGLEHAVITSVN"
BASE COUNT          882 a         1282 c         1488 g          732 t
ORIGIN      
        1 gcgccgggga tactggagat cctggcacgt aacgggataa gggacggcct ggtggtcgac
       61 ctgggatgcg gcagcggcca gtgggcgcgg gagctcgttg acgccggcta tcgcgtcctc
      121 ggcatagaca tctcggagga catgatcgag ctttcgcgca ggagggtgcc cgaggccgag
      181 ttcagggtgg gttctctgtt cgacgccgac atcccaccgt gcggcgccgt cacggcgatc
      241 agcgaggtac tcaactacct gttcgacgag gaagcaggcg gtctgaggcc gctcttcggt
      301 cgcgtcttcg aggcactcgc ccccggcggc gtcttcgtct tcgacgtcct gggtccgggg
      361 caggtgccgc caggcacaac ggcgaagggt ttccgcgtgg gggaggattg ggcggtgctc
      421 tccgagaggg aggaggactc cgggcgggga acgatgacgc gtcggatcgt cagtttcagg
      481 aaggtggggg agcactacag gcgcgacgat gaggtccacc gcgtgcgtct gtacgacccg
      541 tcggagctgt ccgccgagct cggcgaagcg ggcttcaggg taaatacggt gcgcgcctac
      601 ggcgactatc ccctgggtga gaaccacgtg gcgttcgagg cgcgcaagcc agtctgagac
      661 agtggcagga gtggagaccc ccggactggg tataactaca tcatgccgga actgccagag
      721 gtcgagacca taaaaggaga ccttcgcgag ctggtcgtgg gctcgaacgt agagcgcgcc
      781 gaggtcctcg actcctcgct cgtggagcag ccttcgaccg aagagttcat caaccggctc
      841 gatggtgtcc gcatcacagg ggcgcggcgg cgggccaagc atctcatcgt ggagcttgat
      901 tcgggagact cgctcgtctt tcagctcaag atcgggggcc agttgttgct ggtgccgcct
      961 gtcgaggaac cgaatacggc gctcatgctc gtcctgcacc tcgacggcga gaggaggctc
     1021 ttcctcaggg acgagacagg ctttacgcgg gcgcggctac tcgatgccgg ggaactcgag
     1081 gagcggctct ctcctttggg accagagcct ctagaagaag ggttcgacga ggggtatctg
     1141 caacgagtca tcggttcgcg gcgggctcag atcaaaccac tgctactgga tcagaaggtc
     1201 gtctcaggca tcgggaacat ctacgcggat gagatactgt acgatgcgag gctgcatccc
     1261 cgaaggaagg cgaacacgct atcggagggg gaatgggtgg cgcttcacgc ggcgatacgg
     1321 acgaacctcg cggcgggcgt ggagcatcgg gggacgacgg tgcggttgta cagggacgtt
     1381 ttggacaggc cgggcgagca tcagaattac ctgcgggtct tcgagaaaca cgggaggccg
     1441 tgtcctgggt gtgaaggaga ggtagtcagg gaaaaggtag gggggaggcc ttcgcacttc
     1501 tgtccagagt gtcagcggga agatggcccc gaagaagagg gaacgttaga atcgttgtgg
     1561 taaacgtgaa aggagatagg ggtggcggat aagttcgacc tcgtagttat cggcggcggg
     1621 aacggtggat acataccggc gatccgggcc agccagctcg gaatgaaggt ggcactgatc
     1681 gacaagcggg aaggcggtca cctgggcgga acgtgcctga acgtcggctg tatcccgacc
     1741 aaagccttgc tccagacggc ccacctgctg cacgatgcaa agaacggcgc ggacttcggt
     1801 gtcaagataa gcgacgtcga gttcgactat ccgcaggcgg ccaggcgccg cgagcaggtc
     1861 gtcaaccagc tccgcaaggg cgtgcagggt ctgatgaaga agaacaaagt aaccgtatac
     1921 aacggcgtcg gcagcttcgt cgagcccaag aagatcaaag tggagacagc agacggcgag
     1981 gaggagctcg aggccgatca cgtcctgatc tccaccggat caactgtaag caccttgccg
     2041 gggctcgagt tcgacggcga gaaagtcatc tcctcggatg acatcgttac caacgacgag
     2101 agttatccca agtccgtcat cgtccttggc tcaggggccg tcggcgtcga gttcgccagc
     2161 atgtaccacg acttcggtgt cgaggtcacg gtcgtcgaac tgctcgaccg catagtgcct
     2221 ctggaggacc cggagatctc agcagccctc gagaaggaat tcgaggacag gggaatgaga
     2281 gtgctcaccg gcaccaaggc cgatcccgag agcctcgaga agaccgacag cggcgtcaag
     2341 atcaccgtgg ccggggggga cgatggtgaa gagacactcg aagctgaggc gctgctcgtc
     2401 gccgtcggac gaaggaccgt cactgaggac ctcaacctcg acgtgaccaa agtcgagctc
     2461 gacgaccgtg ggatcatcca ggtagacggg tacggacgga caggcgagga cggaatctac
     2521 gcggcgggag atgtgatcgg gggctactgg ctcgcccatg cagcgggcca cgaaggcatt
     2581 atcgccgtcg agcacatggc gggcgagaac cccatgccga tgaaccagga cctcgtccca
     2641 cgcgtcacct tctgccgccc agaggtcgct tccttcggac tcagcaaggc gcaggccgag
     2701 gaagagggct acgaggtcaa ggagtccaag ttcccattca gggctatcgg aaaggcgctc
     2761 atcgagggcg agccgaacgg cttcttcaag atagtggccg acgccgaaac cgacctcatc
     2821 ctcgggatgc acgccatcgg gccgaaggtg acggacctga tcaccgaggg cgtcttcgcc
     2881 aagctcgtcg aaggcacgcc cgaagagctc gccatgacga tccacccgca cccgagcctc
     2941 gccgagatcg tcggcgagtc cgcgatggcc gttgaaggac atccgattca tttctaggtt
     3001 tcaggcatca ggcttcagga ggggtcgcgc gcagcggccc ctttcgcttg tcgcatggta
     3061 ccgcccacga ctcctgaact ttcctgaagc ctgatgcgcc ctgaagagct gccagaactc
     3121 gatgtgatcg agaccgcgcg acccatcctg gagagttatc tcggcgcgga ctcccgtcgg
     3181 ggcatcttgc ttgactagcg acgtggttac gggtctgcac aaccccggag agctcgcctt
     3241 tgatcctgtt cgcgacggct tcggcttccg aaaccctgtc ggagaggtgg cgattggaaa
     3301 tggacgcggc ccgcttcttc gaagactcgg cgccatactt tacggcaagg gcctgtgctt
     3361 cggcatggtc accatggcgc ttcgcagatt cgcggagcag ccggcggatg cgtcggagcg
     3421 accattggcc ggactgccgc ctacgcccga attgctcggc gagatccgtc gctaccacgt
     3481 ccgccagtat atgccgcggg ctgtgctcgc cgccgttaag gattggctgc gttcgcgggg
     3541 cgggaaaccc gatagtgtat tgcgccgact ccggcttgca ggcacggact cgcatctgat
     3601 ctgcttcgga ccggccgtca accggaggtt cctatggtgc cttatgagag cgcatgccgt
     3661 cgcgccgtat cgcgtagaga cggagaaccg ggaggcgcgc gtgtacgtct acgacccgaa
     3721 ctatccggga gacagaggcc ggtatgtgaa ctttcgtcgc gacgggtcgg gacgtgccag
     3781 ggagttcgag tacgagggct tcgggacgcg ttgggggtgg gggatctcac tcttccctca
     3841 ctctgcgata ggcgacacgg cacggcatgg cctccgtgcg ataccataag gcgcaaaatg
     3901 tgagggagat aggagagccg tggctcgcag gaagatcaac ctgaaagtat tggacgacgg
     3961 cacgaagacg ttcgaggtcc gccaccgctg ggaggacagg ccggtacccg cgccccccga
     4021 cggaaccccg acggagtacc tgcctttccg gcagcaatcg aggggagtcc ccggcgcgca
     4081 cggccgtccc gagtggctca aggcacgcat ccccgacggc gaggtctacc gcgacatcaa
     4141 ggagaccatg cgcggcctca acctccacac ggtctgcgag gaagcccgct gcccgaacat
     4201 cggtgagtgc tggaacaacc gcacggccac cttcatgatc ctcggcaacg tgtgtacccg
     4261 ctcctgcggc ttctgcgcgg tcctcacagg caaaccgacg gagctcgacc tcgacgagcc
     4321 ccaccgcgtc gccgacgcag ccagacagat gggcctcgag cacgccgtta taaccagcgt
     4381 caac
//
LOCUS       DATAIR010000239         9026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_383997, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000239 DATAIR010000000
VERSION     DATAIR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383997"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1172)
                     /locus_tag="VHM69_05765"
     CDS             complement(273..1172)
                     /locus_tag="VHM69_05765"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2739935.1"
                     /translation="MAETLTQRIVEAMPFLDRIADIVQPEVQKAVAAGGTTVRNVLDG
                     TPLELPLHPALTDVPVGAWTAAITFDALDIATGSRAMRNSADATLALGVAGGFAAAAT
                     GLSDWRYLSGGARRMGVAHALLNTAGLSLSAASLLLRAAGRRNAGRLAFLAGYSISGT
                     AAHLGGELSYNYGLRVNRNAFESVGPEEFVPVLPESELPEGAMRGVTSDDGANILLSR
                     FSGGEVCAISGVCGHLGGPLAEGDRQGDTIVCPLHNSRFDLCSGRVIDGPAVFPQSRY
                     ETRVRNGSIEVRAAEENVQRKVT"
     gene            complement(1248..2420)
                     /locus_tag="VHM69_05770"
     CDS             complement(1248..2420)
                     /locus_tag="VHM69_05770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2739936.1"
                     /translation="MRKAGDSRIWDAVMITAGFWVLVASLWMEVSYMSGVLDRISTSS
                     MEFHVDFESFWRSAEAMLEGTNIYDTGVRLVNLNPPIWTVLISPLGLLSPLTAYRIFV
                     VISLVVSVGYLAWTVEELRLRPGWTAVGVVMLLLSSPMLATLALGQVYPVLALGLVAA
                     WVADRRDRQEVSGAALGLVVAFKPSLLPVLLWPLARRRWKALVAALVSGAVATLVGVI
                     VLGPGTTLDYIEVLRERPVSAYWDNASLPSAAARLFTENPYAQNAATLPWMVPVGYAL
                     GIAAIVITAMKVRHGPEVGLWALVAASLLASPIAWHNYLVLLGPGILLLLARGMVAPA
                     FLLLALQSIPAQWPLIWNDRGTVAATLAMTLYLYILIAHWLALLAATREPAAENPG"
     gene            complement(2562..2651)
                     /locus_tag="VHM69_05775"
     tRNA            complement(2562..2651)
                     /locus_tag="VHM69_05775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Ser,seq:cga)
     gene            2857..3051
                     /locus_tag="VHM69_05780"
     CDS             2857..3051
                     /locus_tag="VHM69_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739937.1"
                     /translation="MIDTVVYQTLIINFRYTLYTSLRALVRIARLGEFLEAGTLARGA
                     IAGFGVGGVLATIAVPGTPV"
     gene            3382..4665
                     /locus_tag="VHM69_05785"
     CDS             3382..4665
                     /locus_tag="VHM69_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739938.1"
                     /translation="MGQRAREQGQLVGGMSTRASISAATLRGRWLFLARAAWVTIATI
                     TFGLFVASVPGYVSNVLKLGQADWMGAPIEAPAGLVFAFDLLGVLSSVTTVLVCLTLA
                     VVLFWQRSDEWMVMFVSCYLLLYGTIMAGPLEWAEAFYPAWPALAVDVVQPLLLTTPT
                     VALIVLFPDGRLVPRWTRWLILLSIPLVVAISYLPLSYWGVFMTTILLGSIYAQVYRY
                     RHVSTLAERQQTKWVLFGFLLWFLLMVILFVPYSIEMNLPSSSPRPWWTLVNSALWWL
                     ALTIVPLSVSIAVLRYRLYDIDVVINRTLVYGSLTVMLGLVYFGSVASLQYALRVLTG
                     HEEQPQLAIVVSTLVIASLFNPLRHRIQSFIDRRFYRRKYDAAKTLEAFGSRLRDETD
                     LGTLRDDLIGIIRETMQPEHVTLWLRPDSKDRRTN"
     gene            4844..5806
                     /locus_tag="VHM69_05790"
     CDS             4844..5806
                     /locus_tag="VHM69_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2739939.1"
                     /translation="MTKGSTTTGWTARRYALLSIAAAVLTIGLKLGAYYLTGSVGLFS
                     DAAESVVNLVAAVAALWALTFAIRPPDEEHAFGHNKAEYFSSGLESALIIIAAVWIGV
                     TAWDRLIDPQPLQNVGLGLSITLVAAALNGGVALVLLRAGRRLRSITLRADAQHLLTD
                     VWTSAGVVLGVVMVQLTGWLVLDPLIGLLVAANIVWTGFRLLRDTAQGLLDRSLPAED
                     QEIIFKVLSRYEREGIRFHAVRTRAAGQRRFVSMHVLVPGRWTVQQGHDLSEKIEGDV
                     AEELPQSTFFIHVEPSEDPASFEDQSLDRERPGGGPEAQKRGRT"
     gene            complement(5842..8157)
                     /locus_tag="VHM69_05795"
     CDS             complement(5842..8157)
                     /locus_tag="VHM69_05795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX2739940.1"
                     /translation="MEVDRNRLVDIELSENARAVLKKRYLKKNEKGEAIEEPIEMFRR
                     VASNIAEGEFRFKEGEEAQRLYEESRERFLQMMLSRRFMPNSPTLMNAGRELQQLSAC
                     FVLPVEDSIDGIYDTLKHQAIIHKSGGGTGFGFSRLRPKNDLVKSTMGVSSGPVSFMA
                     IYDASTDKIKQGGTRRGANMGILRVDHPDVEEFITCKNENDQINNFNISVAITDEFME
                     AVEADTDFELRNPRDGKVMKTVRARDLFRKIVEGAWLNGEPGVVFIDKINDDNPTPQF
                     PIESTNPCSEAHLPPYDSCNLGSINLERFYREDTGEVDWDELRETVHTAIRFLDNVIE
                     QNNYPLPDIDRMSRGNRRIGLGVMGFADLLIQLGITYDSEEGLAFAERVMKFVDDEAW
                     EESRALADERGVMPNFEGSRHEARGDRVRNATVTTIAPTGTISMIAGCSGGIEPLFAV
                     AFMRRQADMQMPDVNPEFVKLAKDRGFYSEDLMKKVAEHGSVRDVEEVPADVRSVWVT
                     SHDISSGWHVRMQAAFQKYTSMGISKTINLPNDATPEDVEDAYRQAYALNCKGIAVYR
                     DGSRDAQVLSTGKTEKKPGETPTMTPASPEAPTAPPVTPPAGTEGQKQTFSSLAEARN
                     GRPRTLSGVTKKIQTGHGPLYVTMNDDEFGPRECFVILGKPGGTAAAFSDALGRMISL
                     AMTHGATPAELVHQLRGIQDGHPAGVGPNAVLSVPDGVARAMSEHYLIEDSLPDTREL
                     PIIGACPDCGSGLTRQEGCVVCHSCGYSKCS"
     gene            complement(8244..8720)
                     /gene="nrdR"
                     /locus_tag="VHM69_05800"
     CDS             complement(8244..8720)
                     /gene="nrdR"
                     /locus_tag="VHM69_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX2739941.1"
                     /translation="MQCPYCDFEDTKVIDSRLSETKDAVRRRRECLSLSCGKRFTTYE
                     RREPLRLMVIKRDGGKEPFDREKLRSGLAKACAKQQVTDEQVELIVDEIEADLRERRR
                     HEVTSRRLGDMVLMRLRRMDMVAYLRFASVYRQYTDVDQFRTELVRLAGSGIGNSR"
BASE COUNT         1662 a         2853 c         2693 g         1818 t
ORIGIN      
        1 tacaacagag cctcagccct cgcctcgttg ggcgagaagg ccagcgcacg cgccgacctg
       61 cagaaggtct tccaggtcga ctccagccgt tccctccgtc gcgccgccgc cgaagccctg
      121 gcgtcgctgg agaagagata ggtagcattc agcacgccgc ctgcggtggc ttgtcagcac
      181 ttcagcaagt aatgtggttc gttcctccgt agagatcggc aaaggtacta tccagaagtc
      241 gtctcgcaaa acgctcgtaa gtgctgactg ggtcacgtaa cctttctctg cacgttctcc
      301 tcggcagccc tgacctcaat gctcccattg cgcacgcggg tctcgtagcg cgactggggg
      361 aagacggctg gaccgtctat cacccgaccg ctgcacaggt cgaagcggga gttgtgaagg
      421 ggacagacga tcgtgtcgcc ctgcctatct ccctccgcca gcggccctcc gaggtggccg
      481 cagacgccgg agatcgcgca tacttcgccg cctgagaagc ggctgagcag tatgttcgcc
      541 ccgtcatccg acgtcacccc gcgcatcgcg ccctcgggca actcgctctc gggcaagacg
      601 ggcacgaact cctcgggacc aacgctctcg aaggcgttgc ggttgaccct cagcccgtag
      661 ttgtaggaca gctcgccgcc cagatgggcc gccgtgccgc tgatagagta tcccgccagg
      721 aatgccaggc gtccggcgtt gcgccgtccc gccgcccgca ggaggagcga ggcagcgctc
      781 agagaaagcc cggctgtgtt caagagggcg tgggccacgc ccatcctcct cgcccctcct
      841 gagaggtaac gccagtcgga gagtccagtt gccgccgcgg cgaacccgcc cgccacgccg
      901 agcgcgagcg tcgcgtctgc tgaattgcgc atggcccttg agccggtggc gatgtccaga
      961 gcgtcgaagg ttatggccgc cgtccacgcg ccaaccggaa cgtccgtgag cgcggggtgt
     1021 agcggcagct ccagcggcgt gccgtcgagg acgttgcgca ccgtggtccc gcccgccgca
     1081 accgccttct gcacctcggg ctgtacgatg tcggcgatcc tgtccagaaa aggcatcgcc
     1141 tccacgatgc gttgcgtgag ggtttctgcc atctcgtccg cctttccacg aaggaaccct
     1201 tgcccagtac ggaagtatgc cacgccgcca ggagaggctc agtaatccta tcctgggttc
     1261 tctgcggcgg gctcgcgtgt cgcggcaagg agggcgagcc agtgcgcgat caggatgtac
     1321 agatagagcg tcatcgcgag ggtcgccgcg accgtgcctc tatcgttcca gatcagaggc
     1381 cactgcgcgg gaatggactg caatgcgagc agcaggaagg ccggagccac catgccccga
     1441 gccagaagca gcaggatgcc ggggccgagc aacactagat agttgtgcca ggctattggc
     1501 gaggccagaa gcgacgccgc gacgagcgcc cacaacccca cctcggggcc gtgcctgacc
     1561 ttcatggcgg taatgacgat agcggcgatc ccgagcgcgt agccgacggg gaccatccac
     1621 ggaagcgtcg cggcgttctg cgcgtacggg ttctccgtga acagccgcgc cgcagcagac
     1681 ggcagggagg cgttgtccca gtatgcgctg accggacgct ctctcaagac ctcgatgtag
     1741 tcgagcgtcg tccctggccc gagcacgatg accccgacga gcgtggccac ggccccggag
     1801 acgagcgcgg ccaccagcgc cttccaccgc cgccgcgcga gaggccacag gagcacaggg
     1861 agcagcgagg gcttgaaggc aacgacgagg cccaacgcgg cgccggagac ctcctgcctg
     1921 tcccggcggt ccgccaccca cgccgcaacg agcccgaggg caaggaccgg atagacctgc
     1981 ccgagagcca gcgtggcgag catcggtgaa gagaggagca gcatcacgac cccgaccgcc
     2041 gtccaccccg gcctcaggcg tagctcctcc acggtccacg ccaggtaacc taccgatacg
     2101 acgagagaga tcacgacgaa gatcctgtat gccgtcagcg gcgacagcag cccgagcggc
     2161 gagatgagca cagtccagat cggcgggttc aggttcacca gcctgacccc agtgtcgtag
     2221 atgttcgtcc cttcgagcat cgcctccgcc gagcgccaga agctctcgaa gtcgacgtgg
     2281 aactccatcg agctcgtcga gatcctatcc aggaccccgc tcatgtagct gacctccatc
     2341 cagagcgagg ccacaagcac ccagaagcca gccgtgatca tcaccgcgtc ccagatcctg
     2401 gaatcgccag ccttccgcac gacctagatc tccacccaga aataaagcca gaacataaag
     2461 ctcatgatac gcgttttgta cgagccagcc attcagtcaa aaggcttcag gctacaggct
     2521 tcagcaagaa cctcgaccct gattcctgat acctgaatgg gcggagaggg ggggattcga
     2581 acccccgacg ccgggattac cgacgtaacg gttttcgaga ccgccgcatt caaccactct
     2641 gccacctctc cgcggcagag tgtatccact cgaccccgac ccttcaacca ccgctctctc
     2701 ccagacacgg ctagagtcca taaccctgta cctcgacttc cagtttactc agacggccgt
     2761 atcacctcgc ttcccgtcaa cgattctcga cccaaatcca agtgtctgga gagaaaaacc
     2821 tgcaattagc ggatttccgg agtccaacgg gactggatga ttgatacggt cgtctatcaa
     2881 acattaatca taaacttcag gtacactctt tatactagcc ttcgtgcgct ggtgaggata
     2941 gcgcggctcg gagaatttct ggaggctggg acgttggctc gtggcgcgat cgccgggttc
     3001 ggcgtcggcg gagttctcgc gacgatcgcc gtgcccggca cccccgtctg attcattctc
     3061 cgagaatact cgacgcgctc taacctgggg gatcaggaag atattcgccc atcagggacc
     3121 ccggatcgag cgtttcaccg gccttttaag acggtctaac agcgcggttc ataagtattg
     3181 atccacttcg ccccagaggt gcaccttagc gaccaccgtc cgggaagacc ttcctgatgc
     3241 ctgaaacctg ttgcccggct tgtgggagga tcaatccttc cgcaagccgc tgtcagaagc
     3301 tcacttctcg gaaggtggct caacactgac ttcggaacgg ttctcgtaac gtttgggaga
     3361 cggtagtgtt gtagagtggt ggtgggacaa cgggcaagag aacaggggca actagtcggt
     3421 gggatgagca cgcgtgcttc gatatcagcc gctaccctgc gtgggcgctg gctcttcctc
     3481 gcccgcgcag cctgggtcac tatcgccacg ataacattcg gcttgttcgt cgcttctgtc
     3541 cctggctacg tttccaacgt tctcaagctc ggccaggccg attggatggg agctcccatc
     3601 gaggctcctg ccgggttggt attcgcgttt gatctgctgg gtgtgctctc gtccgtgacg
     3661 acggtcctgg tgtgtctgac cctcgcagtc gtgctcttct ggcagagatc agacgagtgg
     3721 atggtcatgt tcgtctcgtg ctacctgctt ctgtacggga cgataatggc cggcccgttg
     3781 gagtgggcgg aagccttcta cccggcgtgg cctgcattag cagtagacgt agttcaaccc
     3841 cttttgctca cgacgccaac cgtagcgctc atcgtcctct ttcccgacgg gaggctcgtc
     3901 ccccgctgga cacgttggct gatcctcttg tcgattccat tggtggtggc gatttcatac
     3961 ctgccactct cctactgggg cgtatttatg accacgatct tgctcggcag tatttatgcg
     4021 caggtatacc gctaccggca cgtttccaca ctagccgagc ggcaacagac caaatgggtg
     4081 ttgttcgggt tcctgttgtg gttcctgctg atggtgattc tcttcgtgcc ttacagtatc
     4141 gagatgaacc ttccatccag cagtccccgt ccctggtgga ctctcgtcaa ttcggccctc
     4201 tggtggctcg cgttgaccat cgtgccactc tcagtgagca tcgctgtcct tcgttatcgc
     4261 ctctacgaca tagacgtcgt gatcaaccgc accctcgtat acggctcgct gaccgtgatg
     4321 ctgggactgg tgtatttcgg gagcgttgcc tccctgcaat acgccttgcg tgttctcaca
     4381 ggtcatgaag aacagccgca actagccatc gtagtctcca ccctggtgat agcctcgctg
     4441 ttcaacccac tgagacatcg catccagtcc ttcatcgatc gtcgcttcta ccgccgcaaa
     4501 tacgacgccg cgaagacgct tgaggcattc ggctcccgac tgagggacga aaccgacctt
     4561 ggcaccctca gggacgactt gatcgggata atcagggaaa cgatgcagcc ggagcacgtc
     4621 acattgtggc tgcgtcccga ctccaaggac aggcggacaa actagcagtc gccttccggg
     4681 ctctgcggag gaatgcatga tgtcgtgatg taagggttgg gccgcggggt ttgtggttag
     4741 gttggtcgac aagatggtag aaagtgggga tgttgttcgg gagcacaagg tccgaggcgg
     4801 ctcattcggg ttcgcgatac gaatgtccca ggatgaggcg gttgtgacga aagggagcac
     4861 gacgaccggg tggactgcgc gccggtacgc cctgctctcc atcgcggctg ctgtattgac
     4921 cattgggctc aagctgggag cctactacct cacggggtcg gtcggacttt tctctgatgc
     4981 ggccgagtcg gttgtcaacc tggtggccgc ggtggcggcg ctgtgggcgc tgaccttcgc
     5041 gatacgcccg ccggacgagg agcacgcctt cggccataac aaggccgagt acttctccag
     5101 tggattagag agcgccctga ttattatcgc tgcggtctgg atcggggtta cggcgtggga
     5161 caggttgata gaccctcagc cgcttcagaa cgtgggactg gggctctcca tcacgctcgt
     5221 tgcggcggcg ctcaacggtg gtgtggcgct cgtgctgttg cgggcgggca ggaggttgcg
     5281 ttcgatcacg ctcagggctg acgcgcagca cctgctcacg gacgtgtgga cctcagccgg
     5341 cgtggtgctc ggcgtcgtga tggttcagct cacaggatgg ctcgtgctgg accccctgat
     5401 aggactcctc gtagctgcga atatagtctg gacgggcttc cggctactgc gcgacaccgc
     5461 gcagggactg ctggatcggt cccttcctgc ggaggatcag gagattatct tcaaggtcct
     5521 ctcgcgctat gagagagagg ggatacggtt ccacgccgtg cgtacgcggg cggccggaca
     5581 gcgccgcttc gtctcgatgc acgtgctcgt gccaggccgg tggaccgtgc aacagggcca
     5641 tgacctctcg gagaagatag aaggagacgt cgccgaagag ttgccccaga gcaccttctt
     5701 catccatgtg gaaccctctg aagaccccgc atcattcgag gatcaaagcc tcgacaggga
     5761 gcgccctggc ggagggccgg aggcacagaa aaggggacgg acctaaccgg tccgtccctg
     5821 gcaggccgcc gtcggtggtc tctaactaca cttcgagtac ccgcaggagt ggcagacgac
     5881 gcagccctcc tggcgggtga ggccgctgcc gcagtcgggg cacgccccga tgattggcag
     5941 ctcgcgggtg tccggaaggc tgtcctcgat caggtagtgc tcggacatgg ccctcgcgac
     6001 gccgtcgggg acggagagga cggcgttcgg tccgacgccc gccggatggc cgtcctggat
     6061 gccgcggagc tggtgtacca actcggctgg agtcgcgccg tgggtcatgg cgagtgagat
     6121 catacgccca agtgcgtctg agaacgcggc agccgtgccg ccgggcttgc cgaggatgac
     6181 gaagcactcc ctcggaccga actcgtcgtc gttcatggtg acgtagagcg gaccatgccc
     6241 cgtctgtatc ttcttggtca caccggagag ggtgcgcggc ctgccgtttc gcgcctcggc
     6301 gagcgaggag aaggtctgct tctgcccttc agtgcccgcc gggggtgtaa ccgggggagc
     6361 cgtcggggcc tcgggggagg ccggcgtcat ggtcggcgtc tctccaggct tcttctcggt
     6421 cttgcccgtg gaaaggacct gggcgtcgcg cgagccatcg cggtagacgg ctatcccctt
     6481 gcagttcagg gcgtaggcct gacggtaggc gtcctcgacg tcctcgggag tcgcgtcgtt
     6541 cgggaggttg atggtcttgg aaatacccat cgaggtgtac ttctggaagg ccgcctgcat
     6601 cctgacgtgc caccctgaac ttatgtcgtg cgaggtgacc cacacgctgc gcacgtcggc
     6661 gggaacctct tcgacgtcgc gcacggagcc gtgctcggcg accttcttca tcaggtcctc
     6721 cgagtagaag ccgcgatcct tggcgagctt cacgaactcc gggttgacgt cgggcatctg
     6781 catgtcggcc tgacggcgca tgaaggccac ggcgaagagc ggctcgatgc cgccggagca
     6841 gccggcgatc atcgagatcg ttcccgtagg agcgatggtg gtgacggtcg cgttccggac
     6901 gcggtcgccg cgggcttcgt ggcgggatcc ctcgaagttc ggcatgacgc cgcgctcgtc
     6961 ggcgagggcc cggctctcct cccaggcctc gtcgtcaacg aatttcatca cccgctcggc
     7021 gaaggcgaga ccctcttccg agtcgtaggt aatgccgagt tgtataagca ggtctgcgaa
     7081 gcccatgacc ccgagcccga tcctgcggtt cccccggctc atacggtcga tatcgggcag
     7141 cgggtagttg ttctgctcga tgacgttgtc gaggaaccgg atcgcggtgt ggaccgtctc
     7201 cctgagctcg tcccaatcga cctcgcccgt gtcctcacgg tagaagcgct ccaggttgat
     7261 cgaaccgagg ttgcacgagt catacggcgg caggtgcgcc tcggaacagg ggttcgttga
     7321 ctctatcggg aactgcggcg tcgggttgtc gtcgttgatc ttgtcgataa acacaacgcc
     7381 gggctcgccg ttgagccacg ccccctcgac gatcttcctg aacaggtcgc gcgccctgac
     7441 ggtcttcatg accttgccgt ctctgggatt tctcagctcg aagtccgtgt cggcctccac
     7501 agcctccatg aactcgtcgg ttatggcgac ggagatgttg aagttgttga tctggtcgtt
     7561 ctcgttcttg caggtgatga actcctctac gtcagggtgg tcgaccctga ggatgcccat
     7621 gttcgccccg cgcctggttc cgccttgctt gatcttgtcc gtcgaagcgt catatatagc
     7681 catgaaagag acggggccac tcgaaacgcc catcgtgctc ttgacgaggt cgttcttcgg
     7741 acgcagacgg ctgaagccga agccggtacc gccgccgctc ttgtggatga tcgcctggtg
     7801 cttgagggtg tcgtagatgc cgtctatgga gtcctcgaca ggcaacacga aacacgcact
     7861 gagctgctgc agctccctgc cggcgttcat gagggtcggc gagttcggca tgaacctccg
     7921 gctgagcatc atctggagga acctctccct cgactcctcg tagagcctct gggcctcctc
     7981 gccctccttg aagcggaact cgccctccgc gatgttcgac gcaacgcgcc ggaacatctc
     8041 gataggctcc tctattgcct cgcccttctc gttcttcttg aggtagcgct ttttgagaac
     8101 ggcgcgcgcg ttctctgaga gttcaatgtc taccaacctg ttacggtcta cctccatacc
     8161 catcctccct attttactgc tagttcctct aattccatcc ccagatccgc ccgcgttccc
     8221 cccggttcac gggcggatcc tatttacctg ctgttgccaa tcccggagcc tgcgagacgc
     8281 acgagctcgg tgcggaactg gtccacgtcc gtgtactgcc tgtacacgga ggcgaaccgc
     8341 aggtacgcca ccatgtccat cctcctgagc cgcatcagca ccatgtcccc gagacgcctc
     8401 gaggtaacct cgtgccgacg cctctcgcgc agatccgcct caatctcatc aactatgagc
     8461 tcgacctgct cgtccgtgac ctgctgcttg gcacacgcct tcgccaaccc cgaccgtaac
     8521 ttctccctgt cgaacggctc tttcccaccg tcgcgtttga tcaccatgag cctcaacggc
     8581 tcgcggcgct cgtacgtcgt gaacctcttc ccacacgata gtgatagaca ctcccgcctc
     8641 cgccgaacag cgtccttggt ctccgaaagt cgcgaatcta tgactttggt gtcctcgaaa
     8701 tcacagtacg gacactgcat ctcattaccc ccatcactcc aacaccacat ctggtaccgt
     8761 gaaaacctag cacactacat tttcgagatg caataccctc agaatgtatc taaccctcag
     8821 gttaaattga cgctaaatcc ccgaatgcct ctacgctagt ggaatacgcg ccaaagtcct
     8881 cagttacaaa atttttcaaa gattttttca aatttacgtg ataatgggga atatcccgca
     8941 aatcacgcac ctttatgttc aggtctactc atggccctaa agtaacaatt gagactaaca
     9001 ttactatgtc agtaactctt tacctc
//
LOCUS       DATAIR010000240         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_15115, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000240 DATAIR010000000
VERSION     DATAIR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15115"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VHM69_05805"
     CDS             complement(<1..743)
                     /locus_tag="VHM69_05805"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2739942.1"
                     /translation="MVEHLSRGSIESLNSPLARVVEVHDALASTQERARELARAGTPR
                     GTLVVAEVQTGGRGRLGRHWGSPKGGLWMSLVLRPGFDASLASRITQTAAVGVAKALW
                     EIGVEASIKWPNDLLAGGKKICGILAESSAEKSAGSSAERRLDYVVLGVGMNANLDPA
                     ALGVPDREVTTIRSELGRNVDLLDLLRTLLSNLDAELGRIEDFGAVLEGWRNLNSTLG
                     EKVRVRRFGETIEGMAVDLSPEGALLLATP"
     gene            complement(736..>1736)
                     /gene="cobT"
                     /locus_tag="VHM69_05810"
     CDS             complement(736..>1736)
                     /gene="cobT"
                     /locus_tag="VHM69_05810"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX2739943.1"
                     /translation="AEAARERHLRLTKPPGSLGRLEELGARLAGMAGENPPPVPESPA
                     VVVCAGDHGVLERGVSPWPQAVTAAMVRNFCNGGAAVNAIAKTVGARVSVLDVGVASE
                     LERHPLLRAAKVRRGTDDLSRGQAMSREEAARAVMAGAGVAEELVESGGVDLLVTGDM
                     GIGNTTPAACLISAFTGYPPEETTGRGTGIDDETFKLKVSVIQEAVGLHAPDPEDPLG
                     VLAAVGGLEHAAIVGVILAGAVYGVPVVLDGVVSNSAALVARAFAPHSVGYAIAGHLS
                     AEPGARIALQSLDLAPLLDLDMRLGEGTGGLLAVPLVQAAARTLGEMATLGEMGIG"
BASE COUNT          289 a          630 c          536 g          281 t
ORIGIN      
        1 ggggtagcga gcagcagagc gccctcgggg ctcaggtcaa cagccatgcc ttcgatagtc
       61 tctccaaagc gcctgacgcg caccttctct cccagcgtag agttcaggtt tcgccagcct
      121 tcgaggacgg ctccgaagtc ctcgatccgg ccaagctcgg cgtcgaggtt cgagagcagg
      181 gtgcggagga gatccagaag gtcaacgtta cgtccaagct ctgaacggat ggtcgtgacc
      241 tcgcgatccg ggaccccgag agcggcggga tctaggttgg cgttcatccc caccccgagg
      301 acgacgtagt cgaggcgcct ctctgctgaa gatccggcag atttctccgc gctcgattcg
      361 gcgaggatgc cgcagatctt cttccccccg gcgagcaggt cgttgggcca cttgatgctg
      421 gcttcgaccc cgatctccca gagcgccttc gccaccccta ccgccgccgt ctgggtgata
      481 cgtgaagcga gactcgcgtc gaacccgggg cgcagcacga gcgacatcca gagcccgccc
      541 ttcggcgacc cccagtgccg cccgagccgg ccccttcctc ctgtctggac ttcagcgacg
      601 acgagggttc cccggggtgt tccggcacga gcgagctcgc gggcgcgttc ctgggtcgag
      661 gccagcgcat cgtgtacctc tacgacgcgg gccagcggac tgttcaggga ctcgatggat
      721 ccccggctca gatgctcaac caatgcccat ctcccccagc gtagccatct ccccgagtgt
      781 ccgggcggcg gcctggacca gaggcacggc gaggagacct ccggtccctt cgccaaggcg
      841 catgtccaga tccagtagcg gggcaaggtc cagggattgg agggcgatac gtgctcccgg
      901 ttctgcggag aggtggcccg cgatcgcata tccaacagaa tgaggggcga atgcgcgggc
      961 gacgagcgcg gcggagttgg agacgacgcc gtcgagtacg acgggcacgc cgtacacggc
     1021 gccagccagg atcacaccta cgatagcagc gtgctccagc ccacccaccg ccgccagcac
     1081 cccgagagga tcttcgggat caggcgcgtg caaccccacc gcctcttgta tgacgctaac
     1141 cttgagcttg aaggtctcgt cgtcgatgcc ggtgcctcgg cccgtagtct cctccggcgg
     1201 atatccggta aaggcggaga tcaaacatgc cgcgggcgtg gtgttaccga tgcccatgtc
     1261 accggtgacc aggagatcta ccccaccgct ctcgacgagc tcctcggcca ctcccgcgcc
     1321 cgccattacg gcccgcgcgg cctcctctct gctcatggcc tgcccacgac tcaggtcgtc
     1381 cgttccacgg cgcaccttcg cggcgcgtag aagcggatgc ctctccagct cggaggcgac
     1441 ccccacgtcg agcacgctca ccctcgcccc caccgtcttc gcgatggcgt tgaccgcagc
     1501 gcccccgtta cagaagttcc tcaccatcgc cgccgtaacg gcctgcggcc acggcgagac
     1561 gccgcgttcg aggacaccat ggtcccccgc gcagaccacc actgccggac tctccgggac
     1621 aggaggcgga ttctctccag ccatccccgc gagccgcgca ccgagctcct ccagccgtcc
     1681 caggctgccc ggcggtttcg tgagccggag gtgtcgctcc cgagctgcct ccgcta
//
LOCUS       DATAIR010000241         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_430324, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000241 DATAIR010000000
VERSION     DATAIR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430324"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VHM69_05815"
     CDS             complement(<1..216)
                     /locus_tag="VHM69_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX2739944.1"
                     /translation="MRFLCDAMLGGLAKWLRAAGYNAYYAREGTDVSDAALTRKALEE
                     SRVLLTSDGGFLERKPVRDGRVGFLKVP"
     gene            complement(218..766)
                     /gene="dut"
                     /locus_tag="VHM69_05820"
     CDS             complement(218..766)
                     /gene="dut"
                     /locus_tag="VHM69_05820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX2739945.1"
                     /translation="MHSGVSPELPDSSSLKPVASYPKPPDATMLAMDFGRPLHVPFRR
                     LVPEASMPERAHAGDAGYDLRSVEEVDLAPGARALVRTGISDAIPDGYPGLVLPRSGL
                     AVRHGISLVNTPGLIDSGYRGEIQVPLINHDREATFRIEVGTRIAQLVLVQAATADFT
                     EVQFLESSSDGRGEGGFGSSGH"
     gene            833..>1882
                     /gene="metG"
                     /locus_tag="VHM69_05825"
     CDS             833..>1882
                     /gene="metG"
                     /locus_tag="VHM69_05825"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX2739946.1"
                     /translation="MSEQRQRYLVTSALPYANGPLHVGQIAGAYLPADALVRYLRARG
                     EDVVYACGTDEHGVPITLTAEREGKDPQEVVDYWWDNMKETFERFYISFDNFSRTSTP
                     LHAKNTQLFYRKLKEGGYISEAESKQMYSPTEGRFLPDRYVEGTCPVCGYKEARGDQC
                     DNCGSWYEAYELIDPHSKVTGGPVEVRETTHLYLDLPKFSERLREYVAGQEHWRTSVK
                     NFILGIIDGGLEKRPITRDIHWGVPVPEPGFEDKRFYVWFDACIGYVSSTMEWAENVG
                     EPDAWREYWQEPETKLIHFIGKDNTVFHTLIWPAQLMGTAEDGDEPFILPYDVPANEF
                     MNLEVVVDGERRAMQM"
BASE COUNT          379 a          586 c          585 g          332 t
ORIGIN      
        1 cggcaccttc agaaagccga ctctcccgtc acgtaccggt ttgcgttcga ggaagccgcc
       61 atcgctcgtc aggagcacac gcgactcttc gagcgctttg cgggtgagcg cggcgtccga
      121 gacgtccgtc ccctccctgg cgtagtatgc gttgtatccg gcggcgcgca gccacttcgc
      181 cagcccgcca agcatcgcgt cacagaggaa ccgcacgcta gtgtcccgac gagccaaagc
      241 ccccctctcc acgaccgtcg gaggaagact cgaggaactg tacttcggtg aagtctgccg
      301 tggctgcctg cacgagaacg agctgcgcga tcctggttcc cacttcgatc ctgaaggtcg
      361 cctcgcggtc gtggtttatg aggggaacct ggatctcacc gcggtagcct gaatctatga
      421 gccctggcgt gttgacgagg gagatcccat gcctcacagc gaggccgctg cgcgggagca
      481 cgagccccgg gtagccgtca gggatggcgt ccgagatccc tgtccgcacc agcgcccgcg
      541 cgccaggcgc aagatccacc tcctcaacgg aacgaaggtc gtagccggca tcccccgcat
      601 gggcacgctc gggcatgctg gcctcaggga cgaggcggcg aaaagggacg tgcaatgggc
      661 gtccgaagtc catcgcgagc atagtagcat cgggaggttt cgggtatgag gccacaggct
      721 tcagactact ggaatcaggt agttcgggag atacaccact atgcaagcct cgccttcgaa
      781 tagcccttct gacggctgat acctgaagcc tgaagcctgt tatcctttat ttatgagcga
      841 gcagagacag cggtacctcg taacctcggc gctgccttat gccaacgggc ctttgcacgt
      901 cggccagatc gccggcgcgt acctgcctgc tgacgcgctc gtgcgctacc tgcgagcgcg
      961 cggcgaggac gtggtctacg cctgcggcac cgacgagcac ggggtcccca tcacccttac
     1021 ggcagagcgc gaggggaagg acccccagga ggtcgttgac tactggtggg acaacatgaa
     1081 ggagactttc gagcgtttct acatctcctt cgataacttc tcgcgcacct cgacgccgct
     1141 gcacgcgaag aacacccagc tcttctaccg caagctcaag gagggcggct acatctcgga
     1201 ggccgagagc aaacagatgt acagcccgac ggagggacgt ttcctgcccg accgttacgt
     1261 cgaggggacg tgccccgtgt gcggttacaa ggaggccagg ggcgatcagt gcgacaactg
     1321 cggctcgtgg tacgaggcgt acgagctcat cgacccgcac tccaaggtaa cgggcggacc
     1381 tgtggaagtc agggagacga cgcatctcta cctcgacctc cccaagttct cagagcgctt
     1441 gagggagtac gtcgcgggac aggagcactg gcgcacttcg gtcaagaact tcatcctcgg
     1501 catcatagac ggaggtctcg agaagcggcc cataacccgg gacatacact ggggcgtccc
     1561 agtccccgag cccggcttcg aggacaagcg cttctacgtg tggttcgacg cctgcatcgg
     1621 ctacgtctca tcgacgatgg agtgggccga gaacgtcgga gagcctgacg cgtggaggga
     1681 gtactggcag gagcccgaga ccaaactcat ccactttatc gggaaggaca atacggtttt
     1741 tcacaccctg atctggcccg cccagctcat gggcacggcc gaagatggtg atgaaccttt
     1801 tattcttccc tacgacgtgc cagccaacga gttcatgaac ctcgaagtcg ttgtcgatgg
     1861 agagcggcgg gcgatgcaga tg
//
LOCUS       DATAIR010000242         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_430413, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000242 DATAIR010000000
VERSION     DATAIR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430413"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1401)
                     /gene="trkA"
                     /locus_tag="VHM69_05830"
     CDS             complement(76..1401)
                     /gene="trkA"
                     /locus_tag="VHM69_05830"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX2739947.1"
                     /translation="MIIGAGEVGLNTARMLSREGHDVVLVEQDEKLVEKASEELDALV
                     ISGNGANPKLLSEAGIKRSDLLIAASSSDEVNIIACLAAKSQGVSRTVARIHNPDYYE
                     TGEPFAREMLGIDFIIHTEQMAAEEIKEALLVPGAINVDSFAGDTIEVAEVILDEGSS
                     AVGQPLRDVRLPDDSLVVGVVRGGEALVPRGGTVLEGRDHVLLISGRRQISEAVGAVA
                     TNTAPVREVTIYGGGRIGLRLALSLEKAGMSIRVIERDEARARYVASQLRRGFVLHDE
                     GISRDFLLQEGVDRTDAFVAVTGDDRANLLAAMYARQLGARMTIAGISRGEFAPLADA
                     LGVDLTISPRMLAAEAILRFVRKGEVIDVALLASGAEMIELRVPERCRVAGRPLSEVG
                     FPEGAIVGALLRDGAVVIPTGSEVLRPGDDAVVFTIEDAVEEVENLFAP"
     gene            complement(1442..>2512)
                     /locus_tag="VHM69_05835"
     CDS             complement(1442..>2512)
                     /locus_tag="VHM69_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX2739948.1"
                     /translation="YLITTSFVTSVLISAQEFEEGASDRALAYLAHEYLGNAFGTIYD
                     LSTITILWFAGASAMAGLLNIVPRYLPRYGMSPEWGRAVRPLVIVYTAISMIVTMFFA
                     ANVTAQAGAYATGVLAMMTSAAFAVTLTTRRNGSRPGSIFFGVITVVFVYALISNIYK
                     RPDGITIALFFIGAIIFISLVSRVSRSLELRQERIDLDETAQRFIEEAANRGEIHLVA
                     HRRRLGTKEEYARKEREQREDNHIPKEEPILFLEVQVEDASEFMDVLEVRGVEVDGHK
                     VLRAVSSVVPNAIAAFLLHLRDITGKTPHCYFGWTEGNPIVYLIRYILFGEGDTAPVT
                     HEVLREAEPIAERRPAIHVGGR"
BASE COUNT          440 a          815 c          785 g          472 t
ORIGIN      
        1 gagggacaag gagcgacaac cctatcgcaa ggatcacacc cgcgttcacc ctgatgatga
       61 cccttgggtt caaggctatg gggcgaaaag gttctcgacc tcttccacgg cgtcctcgat
      121 ggtgaatacg acggcgtcgt caccgggcct gagaacctca ctgcccgttg gaatgacgac
      181 ggctccgtcg cgcaggagag cccccacgat cgctccctca gggaacccga cttcggagag
      241 cgggcggccg gcgaccctgc atcgttcagg gaccctgagc tcgatcatct cggccccgct
      301 cgccagcagg gcgacatcta tcacctcgcc cttgcgcacg aagcggagga tggcttctgc
      361 ggcgagcatg cgcggtgaga tcgtcaggtc gaccccaagg gcgtcggcga gcggtgcgaa
      421 ctcgccgcgg ctaatgcctg cgatggtcat gcgggcaccc agctggcggg catacatcgc
      481 ggcgagaagg ttcgccctgt cgtctcctgt gacggcgacg aaggcgtcgg tgcggtcaac
      541 gccctcctgg aggaggaaat ctcgggagat gccctcatcg tggagcacga aacccctccg
      601 cagctgggac gccacgtagc gcgccctggc ctcgtccctc tctatgaccc tgatcgacat
      661 tccagccttc tccagcgaca gggcaagacg caacccgatg cgcccaccgc cgtagatcgt
      721 cacctccctc acaggtgccg tgttcgttgc gacggcgccc acggcctccg agatctgcct
      781 gcggccgctg atgagcagga cgtggtccct gccctcgagc accgtccctc ccctcgggac
      841 cagcgcctcc ccgcccctca cgaccccgac aaccaggctg tcatccggca gtcgaacgtc
      901 gcgcagcggt tggccaacgg ctgacgatcc ctcgtcaagg atcacctccg ccacctcgat
      961 tgtgtccccg gcgaagctgt cgacgttgat cgcaccaggc accagcagcg cttccttgat
     1021 ctcctctgcc gccatctgct ccgtgtggat tatgaagtcg atgccgagca tctccctggc
     1081 gaaaggctcg ccggtctcgt agtagtcggg gttgtggatg cgcgccacgg tgcgggaaac
     1141 tccctgcgat ttcgccgcca ggcacgcgat gatgttcacc tcgtccgagc tgctcgcggc
     1201 gatgaggagg tcggatctct tgatgcctgc ttcgcttagc agcttggggt tggcgccgtt
     1261 acccgagatc accagcgcgt cgagctcttc ggaggccttt tcgacgagct tctcatcctg
     1321 ttcgacgagc acgacgtcgt ggccctctcg cgagagcatg cgggccgtat tgagacctac
     1381 ttcccctgct ccgataatca cagtgcgcaa cgaaacctcc tgctatccgt cgggcctcac
     1441 ttcatctgcc accgacgtgg atcgccggtc tccgttcggc gatcggctcg gcctcgcgca
     1501 gtacctcgtg ggtgacgggg gccgtgtcgc cttctccgaa gaggatgtag cggatcaggt
     1561 agacgatggg gttgccttcg gtccacccga agtagcagtg cggcgtcttc cccgtaatgt
     1621 cgcgcaggtg gagcaggaag gccgctatgg cgttggggac gaccgagctg accgcgcgca
     1681 gtaccttgtg gccgtccacc tccacgccgc gcacctcgag aacgtccatg aactccgagg
     1741 cgtcctcaac ctggacttcg aggaagagta tgggctcttc cttcgggatg tggttgtctt
     1801 cccgctgctc cctctccttc ctcgcgtact cctccttcgt gcccaagcgg cggcggtggg
     1861 cgacgaggtg tatctcaccc ctgttcgcag cctcctcgat aaagcgttgg gccgtctcgt
     1921 cgaggtcgat cctctcctgc ctcagctcga gcgaacggct gacgcgtgag accagggaga
     1981 tgaagatgat cgccccgata aagaagaggg cgatggtgat gccgtcggga cgcttgtaga
     2041 tgttcgagat aagcgcgtag acgaagacta cggtgatcac gccgaagaag atgcttccgg
     2101 gcctggaccc gttccggcgg gtggtcagcg tgacggcgaa ggccgccgag gtcatcatcg
     2161 cgaggacgcc cgtcgcgtaa gctcccgcct gcgcggttac gttggcagcg aagaacatgg
     2221 tgacgatcat ggagatggcg gtgtatacga ttacgagcgg cctcacagcc cttccccact
     2281 cgggagacat accgtaacgt gggaggtagc gtggcacgat gttgaggagc cctgccatcg
     2341 ccgaagcgcc tgcgaaccag agaatggtta tcgtggagag gtcgtagata gtgccgaagg
     2401 cgtttccgag gtactcgtgc gccaggtacg cgagcgcgcg atcgctggca ccttcctcga
     2461 actcttgcgc ggagatgagc accgaggtca cgaagctggt cgtgatcaga ta
//
LOCUS       DATAIR010000243         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_292557, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000243 DATAIR010000000
VERSION     DATAIR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292557"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VHM69_05840"
     CDS             complement(<1..524)
                     /locus_tag="VHM69_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739949.1"
                     /translation="MEATARKQLTVSQNTRLYKFIRYIEPAVGKASYWVWKGGDVPDG
                     PGAYAFNGKVPDISVIIREGLFCAAVPNLALRCDGKLVPTKGNPHYDGGIAAYFTGFY
                     GPGYFSAYDVPFDLAKAKQWATETRSGVLLGKGYWGPQLSQQGHTGILLPSGYVLQSQ
                     AIPGLNWDSHIDYAR"
     gene            complement(826..1023)
                     /locus_tag="VHM69_05845"
     CDS             complement(826..1023)
                     /locus_tag="VHM69_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739950.1"
                     /translation="MEQRKSEAEVALELMRLVLEQAGSRRLSPTRTLALYHLCKEAVA
                     YTPEGPVTADYLEDNLKKLLQ"
     gene            1176..1844
                     /locus_tag="VHM69_05850"
     CDS             1176..1844
                     /locus_tag="VHM69_05850"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HEX2739951.1"
                     /translation="MGEARGSYVVCPICDHIFKTTNASTYVTVGREADLCPKIPGRPS
                     DGARLIRSAVTMCPRCSFAAGESFTDLDLTFEERYGIEDRLRDDGLLKVFRKGQPPWL
                     GFHAAEVCGKERSLKSRELGDLCLRASWVCRKERERPFESTFQLRALRHFMRALQEND
                     LVGRELSVTTYLVGELNRRLGNHREALNWFVNAGRTTEGDPRTAWLDRLIDRQSKLAR
                     EQAA"
     gene            complement(2140..2352)
                     /locus_tag="VHM69_05855"
     CDS             complement(2140..2352)
                     /locus_tag="VHM69_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739952.1"
                     /translation="MPYGDEVLTKQALDHLLPVFLGRVLQFGTHEVEAEGWPPVHNVA
                     DEDAVFPVRETTPRERGERTDEVEAV"
     gene            complement(2528..3358)
                     /locus_tag="VHM69_05860"
     CDS             complement(2528..3358)
                     /locus_tag="VHM69_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2739953.1"
                     /translation="MKVYRAGSQDVDGERRGGVLRPVLLVLLALFVLALIYLIVPFGG
                     QRAVLLGSDARAGEASRSDTIVVTKAGGGMLAVPRDTLVDIPGVGEDKINAAFASGGP
                     ELTVKTLENLTGVPLEDYVVVHFGGVEEIVDALGGITLEVDKPIRVGIDGRRVYIPSG
                     TQKLDGLEALAYVRYRGGPTADIGRIGRQQKFLRELARESTSPAKLPRLPATARATWR
                     NIDTNMNPLAAARFAVRTRLSGIEDAELYPGEPRYIEGISYWVPDKKAGDRVVSQTIE
                     "
     gene            3455..4306
                     /locus_tag="VHM69_05865"
     CDS             3455..4306
                     /locus_tag="VHM69_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739954.1"
                     /translation="MEKLGTASFEIAEERVAWEGLASSCSPPIRRFAAFMLVGFISFI
                     VVTTFVILFYNIFGPRFIAGQGVTVPRTAFDATMLIGVLVAVGGYLVWLIRSLRSYDA
                     FSAMLRRGGLDPVRPTAGGLRAYSDEQLLALRSRYERMHEGKLKDRLGRTFGFHGDDQ
                     FSLGPLSVLPGTFEMGTLRVEWETQLILRSDEPMPEVSWWTEGRHSLLPRKPDETRKL
                     LYASRYTGDSVRELKRRYGFRTERWHATVPEGKLFDAVRDLESAHRIQLALGRSSRIT
                     LSEPDRS"
BASE COUNT          799 a         1395 c         1383 g          856 t
ORIGIN      
        1 cgcgcatagt cgatgtggct atcccagttc aggccgggta tagcctggga ctggaggacg
       61 taaccagagg gcaggagtat gccggtgtgt ccttgctggg agagctgcgg cccccagtat
      121 cctttaccga ggaggacgcc ggagcgggtc tcggtcgccc actgcttcgc cttggcaagg
      181 tcaaagggca cgtcgtaagc ggagaagtat cccggcccgt agaacccggt gaagtaggcc
      241 gcaatgccgc cgtcgtagtg aggattgccc ttcgtcggga ccagcttgcc gtcgcaacgg
      301 agcgcgaggt tgggcacggc ggcgcagaag agtccctctc ttatgattac ggagatgtca
      361 ggcaccttac cgttgaaggc gtaggctccg gggccgtcgg gaacgtctcc acctttccag
      421 acccagtagg aggccttgcc cacggccggc tcgatgtagc ggatgaactt gtacagcctt
      481 gtgttctggc tgacggtgag ttgcttcctg gcggtggctt ccattacgtt atcaactcct
      541 tctcgtttcg atcgctggcc ccatatcccg gcgcacggat attcggccct cggcgaaacg
      601 tccagaatgt cttactgctc gttcgacaac gggcctcgaa acactctctc acctccgcat
      661 tcttgctccg gcttaagtta ctgcccccat cgtagtgggg gcgaatgaaa ctgcgatgaa
      721 atatcgatga attgacagag aatccaaaaa actttccagg ggcgacaacg cgtggggagg
      781 tcgaacgaag cgactacccc tgtgaggacc gcacaggtct gaaacctatt gaagcagttt
      841 cttcaggttg tcttcgaggt agtctgcggt tacggggcct tccggagtgt aggcgacggc
      901 ctccttgcag aggtggtaaa gggccagggt tctggtcggg gagagcctgc ggctccctgc
      961 ctgttccagg accagccgca tcagctccag ggcgacctcg gcctcgctct tgcgttgctc
     1021 catacggacg ccttcctctc agccgctctc gggtaggaat tataccggcg gaacgcgtga
     1081 ctatggtaga atcggaggcg ctttgatccc ggatttcagc cccaaaaggg agtccgaaac
     1141 gcggccctgc ggcccgctag aggcgggggc cgtctgtggg tgaggcgagg ggatcttacg
     1201 tcgtctgtcc gatctgcgat cacatcttca aaaccaccaa cgcgagtaca tacgtcaccg
     1261 tcggcaggga ggccgatctc tgtcccaaga tcccaggccg cccctcggac ggggcgcgtt
     1321 tgatccggag tgcagtgacc atgtgtccga ggtgctcttt cgcggccggc gaatccttca
     1381 ccgacctcga cctgaccttc gaagaacgct acgggataga ggaccgcctg agggatgacg
     1441 gcctgctgaa ggtcttccgc aagggccagc cgccgtggct aggcttccac gcggccgagg
     1501 tctgcggcaa ggagaggagc ctgaagtccc gcgaactcgg cgacctttgc ctgcgcgcct
     1561 catgggtatg ccgcaaggag agagagcggc ccttcgagag caccttccag ctcagggccc
     1621 tccgtcactt tatgcgcgcc ctccaggaga acgaccttgt cgggcgcgag ctctcggtca
     1681 ccacctacct cgtaggggag ctcaaccgcc gcctcggcaa ccatcgcgag gccctcaact
     1741 ggttcgtaaa cgcgggccgc accacggaag gcgacccccg tacagcctgg ctcgaccgcc
     1801 tcatcgatcg ccagagcaag ctcgccaggg agcaggcagc ttagtaaggc ttcaggtctc
     1861 aggtttcagg catcagccag cgtgtgggag gcgaaactgc cgcacagcgg cgattattac
     1921 gaacggatcc gcgccctgct tgaaggagcg tacctcggta ccgatgatcc gcgtgcgcag
     1981 tcagggtttc gtggcgacga ggctcgttgg gaacgggcta ggcgtcccat tacctccgcc
     2041 atagaccgga acgtgacttt tctcgacgtg ggctgcgcca acggactgtt gttggagtgc
     2101 ctcagccagt ggtccgggga ggaaggatac gggatcgaat catacggcct cgacctcatc
     2161 ggttctctcg ccgcgctcgc gaggcgtcgt ctccctcact ggaaagaccg catcttcgtc
     2221 ggcaacgtta tgaactggcg gccatccttc cgcttcaact tcgtgcgtac cgaactggag
     2281 tacgcgcccc aggaatacag gcaggagatg gtcgagcgcc tgcttcgtga gtacctcgtc
     2341 gccgtagggc atctgatcgt ctgcccctac ggcagctctc gccgccccac accgaaagtc
     2401 aaacccgtcg gagagatcct acgcagttgg gatcacgaag tcaccggcga agccgcaggt
     2461 accgacacaa acggcgtgat cttcacccgc gtcgcgtgga cggacgtacc tgatgcctgt
     2521 agcctattca ttcgatcgtc tgtgaaacga ccctatcccc ggcctttttg tccgggaccc
     2581 agtaagagat gccttctatg tacctgggct cgccggggta gagctcggcg tcctcgatgc
     2641 ctgagaggcg tgtgcggacg gcgaagcgtg ctgctgcgag cgggttcata ttcgtgtcga
     2701 tgttgcgcca cgtggcgcgg gccgtagcgg ggaggcgcgg cagcttggcg ggtgaggtgg
     2761 attcgcgcgc cagctcccgc aggaacttct gctggcggcc tatcctccct atatccgcgg
     2821 tcggtccgcc acgatagcgg acgtaggcaa gcgcctcaag gccgtccaat ttctgagtgc
     2881 ctgacggtat gtatacacgg cgtccatcga taccgaccct tatcggcttg tctacttcga
     2941 gtgtgatccc acccagggcg tcgactatct cctccacccc gccgaagtgc accacgacgt
     3001 agtcctccag cgggacgccg gtgaggttct ctagagtctt tactgtcagc tctgggcctc
     3061 cgcttgcgaa ggcggcgttg atcttgtcct ccccgacacc tgggatgtcc acgagcgtgt
     3121 cacgcggtac ggcgagcatg ccgcctccag ccttggtcac cacgatggtg tccgagcgcg
     3181 acgcctcccc ggcccgcgcg tcgctcccga gcaagacagc ccgctgtccg ccgaaaggta
     3241 cgatgaggta gatgagcgcg aggacgaaga gcgcgagcag gaccaggagt acgggccgaa
     3301 gaactccgcc ccgccgctcg ccgtccacgt cctgacttcc agccctgtaa actttcatcc
     3361 cgccattgta gtgaacgaga gcgaggtcag ggccacacat cggcgtaatc tctaacccac
     3421 cctcgaccgg cgggatgcga gtatactgag ccgcgtggaa aagctcggga cagcatcgtt
     3481 cgagatcgcc gaggagcgcg tggcatggga gggcctggcc tcgtcttgtt ctccgcccat
     3541 acgcaggttc gcggctttca tgctggtcgg tttcatctcc ttcatcgtcg ttacgacctt
     3601 cgtcatcctg ttctataaca tcttcggccc acggttcatc gcgggccagg gtgttacggt
     3661 accgaggact gcgttcgacg cgacgatgct catcggcgtc ctggtcgcgg tcgggggtta
     3721 cctggtctgg ctcatcagga gcctgcgctc ctatgacgcg ttctcagcga tgctacgaag
     3781 ggggggcctg gatcccgtac gcccgaccgc agggggactg agggcatact cggacgagca
     3841 gcttctcgcc ctgcgttcgc gctacgagcg catgcacgaa ggcaagctta aggatcgtct
     3901 cgggcgcacc ttcgggtttc atggcgacga ccaattctcc cttggacctt tgagcgtcct
     3961 ccctggcacg ttcgagatgg gcactctgcg ggtcgaatgg gagacgcagc tgatcctgcg
     4021 ctccgacgaa ccgatgcccg aggtctcctg gtggaccgag ggccgccaca gcttactacc
     4081 tcgcaagccg gacgagaccc gcaagctact ctacgccagc cgctacaccg gcgactcggt
     4141 gcgcgagctg aagcgccgct acgggttcag gaccgagcgc tggcacgcca ccgtccctga
     4201 aggcaagctc ttcgacgccg tccgcgacct cgagagcgcc caccgcatcc agctcgccct
     4261 cggccgcagt tctcgcatca ccttgagtga gccagacagg agttgactct ggggtcgccg
     4321 gccatccccc atggctgcta gagtacgtgg atggagagcg gattcggcgc gacggcgccg
     4381 tatacggtgg gggtcgagga ggagttccag ctcgtagacc ccggcacccg cgc
//
LOCUS       DATAIR010000244         6754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_430642, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000244 DATAIR010000000
VERSION     DATAIR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430642"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..945
                     /locus_tag="VHM69_05870"
     CDS             187..945
                     /locus_tag="VHM69_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2739955.1"
                     /translation="MDDSLLFIQTTSTKIAPATVITLIIVSTIMVLGLLVLIGIFAVR
                     TKAPEGPLVYGNFFVVALGAMTTLIGFLVAFPLLVSGVFKDPTQVIALLSALFGTIVG
                     LVGTFFGVKASSDATKQAHQLASDTISGDTSQPTVTAVSPRDRAVDVLPDARITATFS
                     RDMDRVTINTSTFKLLEQGELTPVPGRVDYNVATRVATLTLSAPLQGGKNYEATITAR
                     VKDLSGNTMAEDHTWHFTVVEQGEDQSRRSLPNR"
     gene            1111..1470
                     /locus_tag="VHM69_05875"
     CDS             1111..1470
                     /locus_tag="VHM69_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739956.1"
                     /translation="MKVIPDRIVTLGFGKYVRADKIVALVPIEEDRGSGRRTLVYVDG
                     IPEPLVAGRTEGTILRDMVGAESGAAELLRELQLQIGQVRPLLKSSIRSEAGLDLDEL
                     SRRITDLTGEQSSPQMF"
     gene            1467..2036
                     /locus_tag="VHM69_05880"
     CDS             1467..2036
                     /locus_tag="VHM69_05880"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2739957.1"
                     /translation="MKSDFYDRDPRHVAVELLGCILSHETQEGRASGVIVETEAYRPE
                     DPACHAYNGPTMRNRNIFGRPGIAYVYLSYGTHNLLNAVCEPEGVGSAVLIRALRPVE
                     GEELMQRRRGRARDLCNGPGRLTQALGVDLAYDGQDLTTGNLTISDGGTPPDGIVATT
                     RIGITRGTELPWRYQVRGEKHVSVPPRII"
     gene            complement(2029..4446)
                     /locus_tag="VHM69_05885"
     CDS             complement(2029..4446)
                     /locus_tag="VHM69_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2739958.1"
                     /translation="MARTYSRKTSRDKGAGGGRRPAARRPVRLPAHRKKRGSASKGLA
                     ARILRVIGIFTLCVLVAGAAFAAGGYLGLVNSVEKLDEPRTFETHPTYIYSAPLGGNE
                     DSRRVIGTVFQGQNRKTASLSQMPPHLLNAVVAKEDERFREHGGVDIWGIMRALWVDI
                     RAGEAVEGASTITQQYVRNAYLSQDRTIIRKIKEALIAIEVERKEDSKDQIIADYLNT
                     VYFGNNAYGVEAAAETYFNKSVEDLSIAESATLVGLLWSPSTLGQDREGAKYQRDLVL
                     RKMFDTGYIPSQDYNEALEVPMPNQWPAAPPTQSGFSGPALTRDFAEYAHGELIERYG
                     VNTVLEGGLSVYTTIDLQMQVTAHDIAYGPSGYLPNAEDPDLALVSIEPGTGRIKTMI
                     GDRDEDSQFSLVTQGRRQPGSAFKPFAFIAALEQGIDPNTKYVSEKKEYEVDVGLDEP
                     EKWVVENYDGIVRGEISLKKALWWSDNTVFADLAMNAGGRGLTNGPEKIADVARRCGI
                     STELPEDPRPSIVLGAYEVSPLDMASAYATIANGGRRVEPTAISRVVSNEGMKDEKVL
                     YVAPEHPEGEQVIDEDIAHKATEIMIGDVTEGIAKQASLKDRPVAGKTGTSENFFDAW
                     FIGYTPELVTGTWMGYAEGGDTLEYVLDYARKLHDLSGGVTPAEIWKTYTQEVLEGRP
                     IKQFEGVKLPEKEEHDDEPVSAPEPGATNATDAPRNRATPRTREAAASPSPARARAAR
                     SSAAPSRAAASSAPPSSAASSVAPRDARAPRAQRSRAERARAASARAARASAPPSSAS
                     PSSVAPR"
     gene            4514..5680
                     /gene="tyrS"
                     /locus_tag="VHM69_05890"
     CDS             4514..5680
                     /gene="tyrS"
                     /locus_tag="VHM69_05890"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2739959.1"
                     /translation="MKAGQELQPIYRNAVDVIPRAELDRRLASGESLRVKLGIDPTAP
                     DIHLGFAVVLKELRAFQDLGHTAVLVIGDYTARVGDPSGRSKTRPILSSEQIEENTRT
                     YLEQAYVILDRDRTEVRHNSEWLAPLTMEDLIHLTRATTVARILERDDFSKRYAANDP
                     ITLTELLYPLMQAYDSVAIDADVELGGTDQLYNLLMGRQVMEYYGKTPQCVVTTPLLV
                     GTDGTIKMSKSVGNYIGVTDPPTEMFGKAMSIPDRLMPEYYSLLLDREPPDAEPVEQK
                     RELARSLVSKFHGQSAVAEAERTFDAVVRREVPEDAPVVELPAGEEIWIIDLITLAGF
                     ANTNGEARRFIKGGAVRLGGEVIRDERRSLPTADLAGLVLQVGKRRYARLEVSP"
     gene            6176..>6754
                     /locus_tag="VHM69_05895"
     rRNA            6176..>6754
                     /locus_tag="VHM69_05895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1280 a         2065 c         2094 g         1315 t
ORIGIN      
        1 tgaagccgca cgaccacgaa tataccgggt ttggcggggc tgcgccatgc tggaaccctc
       61 gtgcccggct ctgaagcgga ctcgcgcagc gaagtagcgg ctcgcgttca cctgagcatg
      121 acgattcatc ctcaattcat cggcgtatgc gacattggcg gttgagcgag cgtggaggat
      181 agacagatgg acgactccct gctattcatt cagacgacgt ctacaaagat agcaccagcg
      241 accgtgataa ccctgatcat agtttcgacc ataatggtgc tcgggctact tgtccttatc
      301 gggatatttg cagtaagaac gaaagcgccg gaagggcctc tagtctacgg gaacttcttt
      361 gttgttgccc tgggtgccat gacgaccctt atcgggttcc tcgttgcatt tcctctgctc
      421 gttagtggcg tgtttaagga tccaacgcaa gtgatagcgc tcctgagcgc cctgtttgga
      481 acaatcgtcg gactggtcgg cacttttttc ggagtcaagg cgagtagcga cgcgacaaag
      541 caagcacatc aattggccag cgacaccatc tcgggcgaca cgtctcagcc cacggtcacc
      601 gcggtgagtc cccgggatcg cgccgtcgac gttcttcctg atgctcgtat caccgccact
      661 ttctccagag acatggaccg cgtgaccatc aacacaagta ctttcaagtt gctcgagcag
      721 ggtgaactca cgcctgtccc cggtagagtg gactacaacg tagccacgag ggtggccact
      781 cttactctgt ctgcccctct acaaggcggc aagaattacg aggccacaat tacggctcgt
      841 gtaaaagacc tttcgggcaa cacgatggcc gaggaccata cgtggcactt cacggtggtc
      901 gagcagggcg aagatcagtc gcgacgctcc ttacctaaca gatgacgggc tggggccccg
      961 cttctcgacg tcgctcgacg ccatcttcca acctatccag gagggccgcg acattccgca
     1021 gcaccaggtc ggtgccgcga attcgtccga cgggtcgctg caagtgggcg gggatgttat
     1081 aagtatttct gacatggaga tttcatccag atgaaggtga taccggaccg tatcgtgacc
     1141 cttggattcg ggaagtacgt gagggccgac aagatagttg ccctcgtccc gatagaggag
     1201 gatcgcggaa gcggcaggcg cacgctcgtc tacgtagatg gcatacccga acctctcgtg
     1261 gcgggccgga cggaagggac catcctcaga gacatggtcg gcgccgagag cggggcggcc
     1321 gagctgctgc gcgagttgca gctccagatc ggccaggtcc gtcctctcct caaatcctcg
     1381 attcgctccg aggcggggct cgaccttgac gagctctccc gccgcataac ggacctcacc
     1441 ggagagcaat catctccgca gatgttctga agtccgattt ctacgacaga gatcccaggc
     1501 atgtcgcggt cgagctactc ggctgtatcc tctcacacga aacccaggaa ggacgcgcat
     1561 ccggcgtgat cgtcgagacc gaagcctacc gtccggagga ccctgcatgc cacgcgtaca
     1621 acggccccac gatgcgcaac cgcaacatct tcggccggcc aggcatcgcc tacgtctacc
     1681 tctcctacgg tacccacaac ctcctcaacg ctgtctgcga gccagagggt gtcgggagcg
     1741 cagtcctgat cagggccctt cgtcccgtcg aaggggagga gctgatgcag cggagacgag
     1801 gccgggcgcg cgacctctgt aacggtccag gccgcctgac gcaggccctc ggcgtggacc
     1861 tggcgtacga cggccaagat ctcacaacag gtaacctgac tatctcggac ggaggaacgc
     1921 ctccggacgg gatagtcgcg acgacccgga tcggcattac gcgcggaacc gagttgccgt
     1981 ggcgctacca ggtccgcggc gaaaagcacg tctccgtgcc acccaggatt atctaggggc
     2041 gacgcttgaa ggcgatgcgc tggaaggcgg tgcgctcgct cgtgcggccc tggcactcgc
     2101 ggccctggcg cgctcagcgc gagatcgttg tgccctggga gctctggcat ctcggggcgc
     2161 gacgctcgac gctgcgctcg atggcggcgc gctcgacgca gcagctctcg acggagcagc
     2221 gctcgaacgt gcggctctag ccctggccgg gctgggactt gccgcagcct cgcgtgttct
     2281 cggggtcgcg cggttacgag gggcatcggt ggcgttcgtc gcgccaggtt ccggggcgct
     2341 tacgggctcg tcgtcgtgct cctctttctc gggtaacttg acgccctcga actgtttgat
     2401 cggccgacct tccaggacct cttgtgtgta ggtcttccag atctcggccg gggtcacgcc
     2461 gcctgagagg tcgtgcagct tacgggcgta gtcgagtacg tattcgaggg tatccccgcc
     2521 ttccgcgtag cccatccagg tgcccgtaac cagttccggt gtatagccaa tgaaccaggc
     2581 gtcgaagaag ttctcgctgg taccagtttt gccggcgacc ggcctgtctt tcagcgaggc
     2641 ctgcttggcg ataccttctg ttacgtctcc tatcatgatc tccgtcgcct tgtgggctat
     2701 gtcctcgtct atgacctgct cgccttccgg gtgctcgggg gccacataaa gcaccttctc
     2761 gtccttcatg ccctcgttgc tcacgaccct ggagatcgct gtcggttcga cccgcctccc
     2821 gccgttagct atggtcgcgt aggccgacgc catgtccagc ggggagacct cgtaagcgcc
     2881 gagcacgatc gagggcctgg gatcctcggg gagttcggtg ctgatgccac acctcctggc
     2941 gacgtcggcg atcttctccg ggccgttggt gaggccgcga ccgccggcgt tcatcgccag
     3001 gtcggcgaac accgtattgt ccgaccacca cagcgccttt ttgaggctga tctcaccgcg
     3061 cacgatcccg tcgtagttct cgaccaccca cttctcgggc tcgtccaggc cgacgtcgac
     3121 ctcgtactcc ttcttctcgg agacgtactt ggtgtttgga tctatgccct gctccaatgc
     3181 ggcgatgaag gcgaagggct tgaaagcgct gccgggctgg cgcctgccct gggtgacgag
     3241 gctgaactgg gaatcctcgt ccctgtcgcc aatcatggtc tttatgcggc ccgtccccgg
     3301 ctcgattgag acgagggcga ggtcgggatc ttccgcattt ggcagatagc cggagggccc
     3361 gtaggcgatg tcgtgggcgg tgacctgcat ttgcaagtct atggtcgtgt acacgctgag
     3421 gccaccctcg aggacggtgt ttacgccgta gcgctctatg agctcgccgt gcgcgtactc
     3481 cgcgaagtct cgggtgagcg caggacctga gaaccctgac tgggtcggcg gcgccgcggg
     3541 ccattgattc ggcatcggga cttcgagtgc ttcgttgtaa tcctggctcg ggatgtaccc
     3601 cgtgtcaaac atcttcctca gaacgaggtc ccgctgatac ttcgctcctt cgcggtcctg
     3661 tcccagggta gacggagacc agaggagccc gaccagggtc gccgactcgg cgatgctcaa
     3721 gtcctcgacc gacttgttga agtacgtctc cgccgcggcc tccaccccgt atgcgttatt
     3781 gccgaagtag acggtgttca ggtagtcggc gatgatctgg tccttcgagt cctctttgcg
     3841 ctctacttca atggcgatga gggcctcttt gatcttcctg atgatggtac ggtcctggga
     3901 gaggtaggcg ttcctgacgt actgctgtgt gatggtactc gctccctcga ccgcctcgcc
     3961 ggccctgatg tcgacccaga gcgcacgcat gatcccccag atgtccacgc cgccgtgctc
     4021 cctgaaacgc tcatcctctt tggctacgac ggcgttgagg agatgcggcg gcatctgcga
     4081 gagcgacgcc gtcttgcggt tctggccctg gaataccgtg ccgatcactc gccgcgagtc
     4141 ctcgttgccg ccgagcgggg ccgagtatat gtaggtggga tgggtctcga aggtccgcgg
     4201 ctcgtcgagc ttctctacac tattgacaag acccaggtaa cctcccgcgg cgaacgccgc
     4261 gcccgcgacc agcacgcaca aagtgaagat gcctatgacg cgcagaatcc tcgcagcaag
     4321 gcccttgctg gcggaacccc gcttcttgcg atgcgccggc aaccttacag ggcgtcgggc
     4381 cgccggtcgc ctgccgccac cggctccttt gtcccgactg gttttcctgg aataggtgcg
     4441 ggccatcgag ccggtatact acactacgcc gacgcaggtt acagtgacat tgcgagaagg
     4501 ttgcgatccg ttaatgaagg ctggtcaaga gctccaaccc atctaccgca acgccgtcga
     4561 tgtgatccca cgcgcagagc ttgaccgccg cctcgcgagc ggcgagtcct tgcgggtgaa
     4621 gctcggtata gaccccacgg ctccagatat tcacctcgga ttcgccgtcg tgctcaagga
     4681 gcttcgtgcg ttccaggacc tcggacacac ggcggtgctc gtcatagggg attacacggc
     4741 gcgtgtaggc gacccgtcgg ggcgttcgaa gacgcgtccg atcctctcct ccgagcagat
     4801 agaagagaac acgcggacgt atctagaaca ggcgtacgtg atcctcgacc gcgacaggac
     4861 cgaggtccgc cacaactcgg agtggctcgc gccgctcacg atggaagacc tgatccacct
     4921 gacccgcgca acgacggtgg cccgcatcct ggagcgcgac gatttcagca agcgctacgc
     4981 cgcgaacgat cctattacgc tcacagaact actctatccg ctgatgcagg cctacgactc
     5041 ggtcgcgata gacgccgacg tcgagctcgg cggcaccgac cagctctaca acctgctcat
     5101 gggccgccag gtgatggagt actacgggaa gaccccgcag tgcgtcgtca cgaccccgct
     5161 cctcgtcggc accgacggga ccatcaagat gagcaagtcc gtcggcaact atataggcgt
     5221 aaccgacccg ccgaccgaga tgttcgggaa ggccatgagc atccccgacc gcctgatgcc
     5281 cgagtactac tctctcctgc tggacaggga accgccggac gccgagcccg tcgagcagaa
     5341 acgcgagctc gcccgctcgc tagtgagcaa attccacggg cagtccgccg tcgccgaagc
     5401 cgagaggacc tttgacgccg tcgtgcgccg cgaggtcccc gaggacgccc ctgtcgtcga
     5461 gttgcccgcg ggggaggaga tctggataat cgacctcatc accctcgctg gtttcgccaa
     5521 caccaacggc gaagcgcgcc gcttcataaa gggcggggcc gtcaggctag gcggcgaggt
     5581 catcagggac gagaggcgct cgctccctac cgcagacctc gcgggtctgg tgctccaggt
     5641 tggcaagcgc cgctatgcgc gtctcgaagt tagtccgtag agcaggttct aggcgctttg
     5701 agcagcgttg cttgacgacg gcgttcgggg gagtataatc ctgcttcgcc gagcagcccg
     5761 aaggtcgtcg agaggcgctg gagggcgcgc ggtagggtgg agaagaaagc ccaaaaaact
     5821 cccgaaactg gtttgacacc ttgggggctg gtgctatact tctctagctg atgacgggga
     5881 actacttgaa aatcgagagc ggcatataag gtaggactcc ctacacattg ggagtgtgta
     5941 cctggcccgc cgaaaggtgg gcgttttagt cgccttcaga aggtagtctt cgtcggctcc
     6001 gccgagaggc aggggcttga ggccttgaaa actgaatagc agtaaaagat tgggtagaga
     6061 acccgtctaa ccgccgagac tgactctcag cggtccgaga gtgtaatgcg gatcgccgag
     6121 cgggtggcgc ccgcaccaac cattacctta aatggtgctt cggcaccatc ggtatagttc
     6181 ggagagtttg atcttggctc aggacgaacg ctgacggtgt gctttaggca tgcaagtcga
     6241 acgagaaagc ccttcggggt gagtaaagtg gcgaacgggt gagtaacacg tgggcaacct
     6301 gcccctcgca gggggacaac cggaggaaac tccggctaat accccgtaag cttgtcgagt
     6361 cgcatggctc gtcaaggaaa ggtagcttcg gccatccggc gagggatggg cccgcggtgc
     6421 attagctagt tggtggggta acggcccacc aaggcaacga tgcgtagctg gtctgagagg
     6481 atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc agcagccagg
     6541 aatcttgggc aatgggcgaa agcctgaccc agcaacaccg tgtgagcgat gaaggccttc
     6601 gggtcgtaaa gctctgttga tagggacgaa gggcgaaggg ttaatagccc ctagcctgac
     6661 ggtacctttc gaggaagccc cggctaacta cgtgccagca gccgcggtaa tacgtagggg
     6721 gcgagcgttg tccggaatta ttgggcgtaa agag
//
LOCUS       DATAIR010000245         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_384524, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000245 DATAIR010000000
VERSION     DATAIR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384524"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VHM69_05900"
     CDS             <1..616
                     /locus_tag="VHM69_05900"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2739960.1"
                     /translation="ESTVEVGDRTYRITHPVSADDLIDEEDFDRDERLPYWAELWPSA
                     IALARHLYERDLSGKRAIELGCGVGLSTIIALARGAEVLATDHYEAALDFTTHNAGTN
                     QEREPEVSVLDWREPDIRGLGVFDLVLAADVLYERKNAAALADLVPKLLAPGGEAIFA
                     DPRRDEAPVFLKAMEEQGFENMMEDSMVEQGTREVKVLLHRMRR"
     gene            665..>2713
                     /gene="pcrA"
                     /locus_tag="VHM69_05905"
     CDS             665..>2713
                     /gene="pcrA"
                     /locus_tag="VHM69_05905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX2739961.1"
                     /translation="MVLLDSLNPTQLDAVERTEGPLLILAGAGSGKTRVLTHRVAYLL
                     DQGFAAPEEVLAITFTNKAAREMKERVALLVGPDSRKMWVSTFHSFCARILRVHAEKL
                     GYKREFTIYDGADQVRLVKRCIVELGKDPKRFNPRSFQAQISSAKNVLMAPDDFLRNT
                     EGYIAENVAEVYDLYQRRLYENNAMDFDDLIMQTVALLEIFPEVRERYQTRFRYIHVD
                     EYQDTNHAQYRLVNILAAQHRNLCVVGDDDQSVYSWRGADIRNILDFERDYPEAKVVK
                     LEQNYRSTQTILDAANAVVANNASRKPKALWTAGDEGERIRVFAASDEYAEARFVVSE
                     IERLTDSGASAREISVFYRTNAQSRALEDVLVREGVPYQVVGGVRFYERAEIKDAMAY
                     LSVISNPDDSVSLERIINVSKRGLGATSVAKLQDYARRNGVSLYEAISEVDAVDLTGA
                     AKKACRAVRDLFEGWRVAAREVPPAELIGAVLDESGYRKELEAEDTVEAESRLENLEE
                     LINAAREYERVEPEPTLGGFLQEQALYSDQDSLTSEGGSVTLMTLHNAKGLEFGHVFV
                     VGMEEGTFPHARSLDEHNLEEERRLAYVGITRARKTLTLTHAKLRSSWGEREYRMPSR
                     FLSEIPDQYKSGTVPAGSFMNTSGRGGWGVAVPGRKGPERAASGVVAYRTGEKVRHAK
                     F"
BASE COUNT          546 a          837 c          878 g          452 t
ORIGIN      
        1 cgaatccacg gtcgaggtcg gagacaggac ctatcgcatc acgcatcccg tctccgccga
       61 cgacctgatc gacgaggaag acttcgaccg cgacgagcgg ctcccttact gggccgagct
      121 ctggccgagc gccatcgccc tagcgcgaca cctctacgaa cgagacctca gcggcaagcg
      181 cgccatcgag ctgggctgcg gcgtggggct gtccaccatc atcgccctcg cccgcggcgc
      241 ggaagtactc gcgaccgacc actacgaggc cgccctcgac ttcaccaccc acaacgccgg
      301 cacgaaccag gagcgggaac ccgaggtctc tgtactcgac tggcgcgaac cggatatcag
      361 gggtctcggc gtcttcgacc tcgttctcgc cgctgacgtc ctctacgaac gcaagaacgc
      421 cgcggccctg gcggatctcg tacccaagct cctcgcgcca ggcggcgagg cgatcttcgc
      481 cgacccccgt cgagacgaag ctccggtctt cctgaaagcg atggaggagc aaggtttcga
      541 gaatatgatg gaagattcga tggtggagca gggtacgaga gaggtgaaag tgctcctgca
      601 caggatgcgg cgctgaacga gtactgccgg cgggatctga aggtccctgc tgttatactc
      661 tcatatggtg cttctcgact cgttaaaccc gactcagctt gacgctgtag agcgcacaga
      721 agggccgctc ctgatcctcg ccggggcagg gagcgggaag acgcgcgtgc tcacgcatcg
      781 cgtcgcctac ctcctcgacc agggattcgc cgctcccgag gaggtcctgg ccataacctt
      841 cacgaacaag gccgcccgcg agatgaaaga aagggtggcc ctcctcgtcg gccccgattc
      901 gaggaagatg tgggtctcta ccttccactc cttctgcgcg cgtatcttgc gggtgcacgc
      961 ggagaaactc ggctacaagc gggagttcac gatctacgac ggcgccgacc aggtcaggct
     1021 cgtcaagcgg tgcatcgtcg agctcggcaa ggaccccaaa cgcttcaacc cgcgctcttt
     1081 tcaggcccag atcagttccg caaaaaacgt cctgatggca cccgacgact tcctccgcaa
     1141 cacggagggg tacatcgcgg agaacgttgc cgaggtctac gacctctacc agaggcgtct
     1201 ctacgagaac aacgccatgg acttcgacga cctgatcatg cagaccgtcg ccctcctcga
     1261 aatcttcccc gaggtccgcg aacgctacca gacgcgtttc cgctacatcc acgtggacga
     1321 atatcaggac acaaaccacg cccagtaccg tctggtcaac atcctggcgg cacaacatcg
     1381 taacttgtgt gtcgttggtg atgacgatca gtcagtgtac tcatggcgcg gagcggatat
     1441 ccggaacatt ctggacttcg agcgggacta tcccgaggcc aaggtcgtca agctggagca
     1501 gaactatcgt tcgacgcaga ccatactgga cgcggcgaac gcggtcgttg cgaacaacgc
     1561 ctcgcgcaag cccaaggcgc tgtggacagc gggggatgag ggggagcgca ttcgggtgtt
     1621 cgcggcctcg gacgagtatg cggaggcgcg gttcgtagtc tcggagatcg agcgtctgac
     1681 cgactctggc gcttctgcgc gggagatctc ggtcttctac aggacgaacg cccagagcag
     1741 ggctctggag gacgtgctgg tgcgcgaggg tgtaccttac caggtcgtcg gcggggtcag
     1801 gttctacgag cgggcagaga tcaaagacgc catggcctac ttgtcggtga tctccaaccc
     1861 cgacgactcg gtctctctgg agcggatcat caacgtgtcg aagcgaggcc tcggcgcgac
     1921 ctcggtggcg aagctccagg actacgctcg caggaacggc gtctcgctct acgaagcaat
     1981 atcggaggtt gacgcggtcg acctgacggg ggccgcgaag aaggcctgcc gggctgtacg
     2041 cgacctgttc gagggctgga gggttgcggc gagggaggtg ccgcccgcgg agctgatcgg
     2101 ggccgtcctc gacgagtccg gctacaggaa ggaactcgag gcagaagata ccgttgaggc
     2161 tgagtcgcgc ctcgagaacc tcgaggagct gataaacgca gcaagggagt acgagcgcgt
     2221 ggagcccgag ccgacgctcg gcggtttcct gcaggagcag gccctgtact ccgaccagga
     2281 ttctctgacc tcggagggcg ggagcgtcac tttgatgacg ctccacaacg ccaaggggct
     2341 cgagttcggg cacgtattcg tcgtcgggat ggaggagggg accttccctc acgcccgttc
     2401 gctcgacgag cacaacctgg aggaggagcg gaggctcgcc tacgtcggca tcacgcgcgc
     2461 caggaagacg ctcacgctga cgcacgcgaa gctgcgctct agctggggcg agcgcgaata
     2521 caggatgcca tctcgcttcc tctcggagat cccggaccag tacaagtcag gcacggtgcc
     2581 tgctggcagt ttcatgaaca cctcggggcg cggaggatgg ggcgttgccg tgccgggacg
     2641 gaaaggtccc gagagagcgg cttcgggagt cgtggcctac aggacagggg agaaggtcag
     2701 gcacgcaaaa ttc
//
LOCUS       DATAIR010000246         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_430766, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000246 DATAIR010000000
VERSION     DATAIR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430766"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..662)
                     /locus_tag="VHM69_05910"
     CDS             complement(135..662)
                     /locus_tag="VHM69_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2739962.1"
                     /translation="MQEMPDLRDVPIFKHLDADERAEFEELLEPASFETGEMIFEEGG
                     PEERLYVITSGTVEVYKSILPGRRQHLATIGAPTVVGEMGLLTEPRAAASVEATSNIE
                     AHGVDRDRLLEMLDADSPAACKLVYEIGRTLAERMAATNATVAEVISRLENADAGGLG
                     DVDLFRDQLVQDWSF"
     gene            complement(675..1199)
                     /locus_tag="VHM69_05915"
     CDS             complement(675..1199)
                     /locus_tag="VHM69_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2739963.1"
                     /translation="MMEEVLQGVRFFEFLTEEEREEFADVSEPVSFGAREDIIEEGTE
                     PGNLFVLISGTVEVRKGLPGGRSRVLAEIDAARERTIVGERGLLGETGASATVRAVGE
                     VEAIKIPRGTFRRMVEEGRPAAFKLSYRIARTLARRLTRLDEEVVEVIRELETRGETD
                     MEAFRDRLITDWSV"
     gene            complement(1380..2078)
                     /locus_tag="VHM69_05920"
     CDS             complement(1380..2078)
                     /locus_tag="VHM69_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2739964.1"
                     /translation="MARAGQEIYNPVQNDWIVFRQTAQDTGGELMRGELIVSPGGGNP
                     LHVHPLQEEHFKALSGTLGVQMGEDHRSLEEGEEAVVPPGTPHRWWNAEDQAARVLVE
                     LRPALNTEIFFETLYGLARDGKTDENGVPNLLQQAVTLNGINKGEVYLARPPIPVQKA
                     SLAVLAPVGRMLGYKDHYPKYSGAETPSGEGAGPPSKGSVMARGVAMVASLLVAFLFL
                     RGLMRRHVRAELRR"
     gene            complement(2247..2759)
                     /locus_tag="VHM69_05925"
     CDS             complement(2247..2759)
                     /locus_tag="VHM69_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2739965.1"
                     /translation="MAAKEDGKIFIGPYEGVHLPTLDIAHKVPSESFGGALTIIEWSL
                     SPGVLIPPHTHSREHECNYVLQGELTCDIGGEIVVAPVGSYVVKPRGVYHALCNAGTE
                     PVRVIEIHVPGGFERWYDEYEEIASKFALGEIDEGEHLRARADLSERYGVIFHDERIP
                     ETRARFGIGP"
     gene            complement(2913..3350)
                     /locus_tag="VHM69_05930"
     CDS             complement(2913..3350)
                     /locus_tag="VHM69_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2739966.1"
                     /translation="MSAVDDLDQEVEQYQVALGEFMKGNSEPAKKVWSHREDVTLANP
                     LVGSPARGWEQVAKTMEHAASQVRDGEITGFETVAQYVTPDLAYVVWVERARAKVAGR
                     EDVVPLDLRVTMIFRPEDGEWKIVHRHADPITTAQPAESVIQQ"
BASE COUNT          623 a         1117 c          913 g          716 t
ORIGIN      
        1 gggagacctc gtgaacgccg tagacaggat cgcacagagc aacgacggaa gccccggcgt
       61 gaagagccgc ggcacgggtc aggacagctg atacctgatg gagcgatttt cagaagtacc
      121 tcctgaaaat cgctctagaa actccagtcc tgaacgagct ggtcgcggaa caggtcaacg
      181 tcccccagcc ctccggcgtc cgcgttttcg agacgggaga tgacctcggc caccgtggca
      241 ttcgtcgctg ccatacgctc tgcgagggtt cggcctatct cgtagacgag cttgcaggcg
      301 gccggggagt cggcgtcgag catctcaagg agacggtccc tgtccacgcc gtgggcctcg
      361 atgttcgatg tcgcctcgac gctcgccgcg gccctgggct cggtcagtag tcccatctcg
      421 cctaccacgg tcggagcccc gatcgtagca aggtgttgcc tgcgtccggg gagaatactc
      481 ttgtacacct cgactgtgcc tgaggtgatc acatacagac gctcctcggg gccgccctcc
      541 tcgaagatca tctcccctgt ctcgaagctc gcaggttcga gaagttcctc gaactcggcc
      601 cgctcgtccg cgtcgaggtg cttgaagatc ggcacgtccc tcaggtctgg catctcttgc
      661 ataaaagagc tcctttacac ggaccagtcc gtgatcagcc tgtcgcggaa ggcctccata
      721 tccgtctccc ccctggtctc gagctccctg atcacctcaa cgacctcttc gtccagacgc
      781 gtgagcctcc tcgcgagggt gcgggcgatg cggtatgaga gcttgaaggc ggcgggcctc
      841 ccttcttcga ccatgcgcct gaaggtgccg cgtgggattt ttatggcctc gacctcgccc
      901 acggccctga ccgtcgccga cgctcccgtc tccccgagaa gccctctctc tcctacgatc
      961 gtccgctcgc gcgcggcgtc gatctcggcc aggaccctgc tacgtccccc gggcaacccc
     1021 tttctgacct cgacggtgcc cgagatgagt acgaacaggt tcccaggctc agtaccctcc
     1081 tcgatgatgt cctccctcgc cccgaaggag acaggctccg agacatcagc gaactcctcc
     1141 cgctcctcct cggtcaggaa ctcgaagaac ctgacgccct gtaacacttc ctccatcatc
     1201 gcatcaccct gtatctcaac ctggcaactc ctactcaaga ttatatgcgt cccgttaccc
     1261 cgacgaaacg ccacggctca ctttacccaa cgcccgccat ttgtttccta catctcgtca
     1321 cccttgcctg aatagcgtag gatgctggga ccgcgcaacc tgtgagccct taggttgtcc
     1381 taccgccgaa gctcggcgcg tacatggcgc cgcatcagcc cgcgcaagaa taagaacgcc
     1441 accaatagcg aagctaccat ggctactccc ctcgccatta cactcccctt ggacggcggc
     1501 ccggctccct cgccactcgg agtttccgct ccactgtatt tcgggtagtg gtctttgtac
     1561 ccgagcatcc tccccaccgg agccagcacc gccagggacg ccttctgtac cggtatgggt
     1621 ggccttgcga ggtagacctc acccttgttg atgccgttga gcgtgactgc ctgctgcagg
     1681 aggttgggta cgccgttttc gtcggtcttg ccgtctcggg caagtccgta gagagtctcg
     1741 aagaagatct ccgtgttcaa agcaggccgc aattcgacca agacgcgggc cgcttggtcc
     1801 tcggcattcc accacctgtg tggcgtgccg ggcgggacga ccgcctcttc cccctcctca
     1861 aggcttcggt gatcttcgcc catctgaact ccgagtgtgc cggagagggc cttgaaatgc
     1921 tcctcttgta gaggatggac gtgcaacggg ttgccgccgc cgggcgaaac gataagctcg
     1981 cccctcatca gctccccacc cgtatcctga gcggtctgcc tgaacactat ccagtcattc
     2041 tggaccggat tgtagatctc ctgtcccgcc ctcgccataa tgtcctacct cctcgctctc
     2101 cttggtaaaa gtatggcagt agatgaggta aatgctaccc gggaaggcgt gtttttggga
     2161 ctttcatagc gagaagacaa attatcgaga gagccccatg tacgcgtact tcagaaggat
     2221 gggccacagt cggcactatg gtgcgactaa ggcccgatgc cgaagcgggc tctagtctcg
     2281 gggatgcgct catcatgaaa gatcaccccg tagcgctcgc tgaggtcggc tctagccctt
     2341 agatgttcgc cctcatcgat ctcccccaac gcaaacttcg acgcaatctc ctcgtactcg
     2401 tcgtaccacc tctcgaagcc gcctggtacg tggatctcga tgacccggac gggctcggtg
     2461 cccgcgttgc acaaggcatg gtagacccct cttggcttta ccacgtagga gccgaccggg
     2521 gcgaccacga tctcgccgcc gatgtcgcac gtcaactcac cctgcaatac atagttgcac
     2581 tcatgctcgc ggctgtgggt atgcggaggt atcaacacgc cgggagagag gctccactcg
     2641 atgatcgtga gcgcaccgcc gaagctttct gatgggacct tgtgggctat gtccagggtg
     2701 ggaaggtgga cgccctcgta cggaccgatg aagatcttcc cgtcctcttt cgccgccacg
     2761 ggatgctccc ttccgcttcg catacgtaac gaaggtatcg tagaccggaa gtgaagccgg
     2821 cgcatgcccc aattgagtga tcttcgcctc agctgctcct cgaacttctc agattctcgg
     2881 aatctggttg tggaggttcg ccgagggtag agctattgct gaataaccga ctctgctggt
     2941 tgagccgtgg taatcgggtc cgcgtgtcta tgcacgatct tccactcgcc gtcctcgggt
     3001 ctaaagatca tcgtaacccg cagatccaat ggaacgacgt cctcccttcc ggcaaccttg
     3061 gccctggccc gctctaccca cacaacgtag gcaagatccg gagtcacgta ctgcgccacg
     3121 gtctcgaagc cggtgatctc gccgtctctg acctgcgatg cggcatgctc catggtcttg
     3181 gcaacctgct cccatccacg cgcaggagag cctactaagg ggttagcgag ggttacatct
     3241 tctcgatggg accatacttt cttcgcgggc tcggagttcc ctttcatgaa ctcgcccagc
     3301 gctacttggt attgctcaac ttcctggtca agatcatcta ccgcggacac ctgtagagat
     3361 cacctccat
//
LOCUS       DATAIR010000247         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_329984, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000247 DATAIR010000000
VERSION     DATAIR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329984"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VHM69_05935"
     CDS             complement(<1..1541)
                     /locus_tag="VHM69_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX2739967.1"
                     /translation="MLVKEELLDKVVARIREQMPEDQAPQVEEFARQYYGWVDEDDLE
                     DRSDIDAYGAAVSHWSFAGRRAPGEWKIRIYNPHFDEHGWQSTHTVIEMVNDDMPFLV
                     DSTRMEINRQGYAIHMILHPVIKVRRDGEGRLLEVLPPDSEDEDAISESVIHVEVDRQ
                     TESSELEDLKECIQKVLADVQAAVEDWPDMRENVGDLVSWLENDPPDLEPEDLAEARA
                     FLKWVDDNNFTFLGYREYDLTTQEGEDVLCAVQDSGLGILRQTRAGPVSHSFAELPPE
                     VRRLARTPKLLNLTKANSRATVHRPSYLDYIGIKKFDESGEVTGEKRFLGLYTFTAYS
                     SSAFDIPLVRRKVRYVLERAGFPEGSHNEKDLIEILETYPRDELFQISKEELFDMAMG
                     ILHLQERQRVKIFVRRDTYGRFFSCLVFVPRDRYNTVIRERMQDILLSGFDGENVEFN
                     VRLSESVLARIHFIIYTKPGEIPEYEEEEIEVRLVETTRSWPDNLYDALIEQYGEERG
                     TELFH"
     gene            complement(1677..>3179)
                     /locus_tag="VHM69_05940"
     CDS             complement(1677..>3179)
                     /locus_tag="VHM69_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX2739968.1"
                     /translation="VMLVLCTTVLGASAQENPGGSGGFVALSGEEASKYDVPGDVREV
                     WRASYAGGLAQIRYQQYVGDAAVYGGEITVLLKDGEQVAVTGNHYPNLQPSNEVDVTK
                     ADARNAVQRERGNSGRFVTELFINPETGLRFYQVDSQRDDSRRVFHINAQTGKKIKEY
                     GNLQETEETPSDPGIGVKGDTKQIDTTFNGTVHEMISDDDRQATYDAQNKKMLPGLLF
                     ADPDGTWDSAGRISPGQPAGVDAHYYANVTDDYYTDIFSRNSLDDQGMQMVSTAHFSK
                     DYNNAFWNGQQITYGDGDGVATFRELSGGLDVATHEFTHGVTEFTSGLIYQNESGALN
                     EAFSDMLGNSSEFYAQTNGLDPAGRPDWYIGEDIYLPRDEKPGFRNMADPAEDQDPDH
                     YSERFLGKADNGGVHINSGIPNHAYYLLVNGGSNAGEARGHDHTGPKVKGIGLPDAEQ
                     IFYAGFTSLPSTATMSQARQATEAAAGALYGERSRQKTSTSNAWEAVGVS"
BASE COUNT          494 a         1003 c          978 g          704 t
ORIGIN      
        1 ctgtggaaca gctccgtgcc ccgctcctcg ccgtactgct cgatcagggc gtcgtagagg
       61 ttatccggcc acgagcgggt cgtctcgacg agccgtactt cgatctcctc ctcttcgtat
      121 tccggtatct ctcccggctt cgtgtatatg atgaagtgga tacgggcgag cacggactcc
      181 gagagcctga cgttgaactc gacgttttcg ccgtcgaagc cgctgaggag tatgtcctgc
      241 atgcgctccc tgatcaccgt gttgtaacgg tcgcggggta cgaagacgag gcaggagaag
      301 aagcgcccgt aggtgtcgcg cctgacgaag atcttgacgc gctggcgctc ctgcaagtgt
      361 agtatgccca tcgccatatc gaagagctct tctttggaga tctggaaaag ctcgtcccgt
      421 ggataggtct ccaggatctc gatgaggtcc ttctcgttat ggctcccctc cgggaatccg
      481 gccctctcga gcacgtaccg caccttgcgc ctgacgagcg ggatgtcgaa ggcgctggag
      541 ctgtaggcgg tgaaggtgta aagacccaga aaccgcttct cccccgtgac ctcgcccgac
      601 tcgtcgaact tcttgatgcc tatgtagtcg aggtaggatg gcctgtgaac cgtcgcgcgc
      661 gagttcgcct tcgtcaggtt gaggagcttg ggcgtgcggg cgagcctcct cacctcgggc
      721 ggtagctccg cgaagctgtg cgagacaggc cccgccctcg tctgcctcaa gatgccgagc
      781 cctgaatctt gcacggcgca gaggacatcc tcaccctcct gcgtggtcag gtcgtactcg
      841 cggtagccga ggaaggtgaa gttgttgtca tctacccact tcaagaacgc gcgggcctca
      901 gccagatctt caggctccag atcaggcggg tcattttcga gccaggagac gagatctccg
      961 acattctcgc gcatatcggg ccagtcttcg acggccgcct gcacgtcggc gagtaccttc
     1021 tggatgcact ccttcaagtc ctccagctca gacgactcgg tctggcggtc cacctcgacg
     1081 tggatcacgg actcggagat ggcgtcttcg tcctccgagt cgggggggag gacttcgaga
     1141 agccgcccct ccccgtcgcg gcgcaccttt atgaccgggt ggaggatcat gtgaatggcg
     1201 taaccctggc ggtttatttc catcctcgtc gagtcgacca ggaagggcat gtcgtcgttg
     1261 accatctcga tgacggtatg ggtcgactgc cagccgtgct cgtcgaagtg ggggttgtag
     1321 atccggatct tccactcccc gggcgcgcgc cgcccggcga agctccagtg agagaccgcg
     1381 gccccgtacg catctatgtc cgaacggtcc tccaggtcat cctcatcgac ccatccgtag
     1441 tactggcgcg cgaactcctc cacctgcggt gcctggtcct caggcatctg ctccctgatt
     1501 cgggccacga ccttgtccag aagctcctct tttaccagca tcggcacgcc tccctcggcg
     1561 gttcccttct acctggaagc ttcattatag tctggcgata gtttcccggt caggggcatg
     1621 aagagaggct ccccggtacg gagagcctcc ggctttcgac tgtttgacgt ttgtttctag
     1681 ctgactccca cggcctccca ggcgttgctg gtcgaggtct tctgcctcga cctttcgccg
     1741 tagagggcgc ctgccgccgc ttcggttgcc tgccgagcct gggacatggt cgcggtcgat
     1801 ggcaggctcg tgaagcccgc gtagaagatc tgctccgcgt cagggaggcc gatgcctttc
     1861 accttcggtc ctgtgtggtc gtggccgcgc gcttccccgg cgttactgcc gccgttgacg
     1921 agcaggtagt aagcgtggtt ggggatgccg ctgttgatgt gcacgccgcc gttgtccgcc
     1981 tttccgagga aacgctcgga gtagtggtcc ggatcctggt cctcggcggg atcggccatg
     2041 ttgcggaacc cgggtttttc gtccctgggt aggtagatgt cctctcctat ataccagtcg
     2101 ggcctgcccg ccgggtcgag gccgtttgtc tgggcgtaga actcactgga gttgccgagc
     2161 atgtccgaga acgcctcgtt cagcgcgcct gactcgttct ggtagataag acccgaggtg
     2221 aactcggtga cgccgtgggt gaactcgtgc gtcgccacgt ccaatccgcc agaaagctcg
     2281 cggaacgtgg ccactccgtc accgtcgccg taggtgatct gctgtccgtt ccagaacgcg
     2341 ttgttgtagt ccttgctgaa gtgggccgtc gagaccatct gcattccctg gtcgtcgagg
     2401 ctgttgcggc tgaagatgtc cgtgtagtag tcgtcggtga cgttcgcgta gtagtgggcg
     2461 tcgacgcccg ctggctggcc cggcgagata cgtcccgccg agtcccacgt gccgtcgggg
     2521 tcggcgaaga gcaagcctgg cagcatcttc ttgttctgcg cgtcgtaggt cgcctggcgg
     2581 tcgtcgtcgg agatcatctc gtggaccgtg ccgttgaacg tggtgtctat ctgcttggtg
     2641 tcgcccttga cgccgattcc gggatcgctc ggggtctcct ccgtctcctg gaggttgccg
     2701 tactctttga tcttcttgcc cgtctgcgca ttgatgtgga agaccctgcg cgagtcgtcc
     2761 cgttgggagt ctacctggta gaacctgagt ccggtctcgg ggttgatgaa gagctccgta
     2821 acgaacctgc cgctgttgcc cctctcgcgc tgcacggcgt tgcgcgcatc tgccttcgtc
     2881 acgtcgacct cgttggacgg ctggaggttc ggatagtggt tgcccgtgac ggccacctgc
     2941 tcgccatcct tgagcaggac cgtgatctca cccccataga ccgccgcatc cccgacgtac
     3001 tgctggtagc gtatctgtgc gagcccgccg gcatacgacg cccgccacac ctcgcgcacg
     3061 tctccaggaa cgtcgtactt gctggcctcc tccccggaca gggccacgaa cccgccggag
     3121 ccgccgggat tctcctgggc cgaagcacct agtacggtcg tacacaacac caacatcac
//
LOCUS       DATAIR010000248         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_107845, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000248 DATAIR010000000
VERSION     DATAIR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107845"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHM69_05945"
     CDS             complement(<1..426)
                     /locus_tag="VHM69_05945"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2739969.1"
                     /translation="MREGNGVTSDGAGPMGEREFLVGAALDNLPTRVAIIDESGAIVA
                     VNRAWHEFAATNDGAAIATEGVNYLRACEAATGDGAREAARFAEGVRLVLSGQRQEFT
                     MDYPCHSPGERRWFVGWVARLLVGDARWAVISHEDITGRK"
     gene            961..3138
                     /locus_tag="VHM69_05950"
     CDS             961..3138
                     /locus_tag="VHM69_05950"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2739970.1"
                     /translation="MARPRLLDLLGESLHRKATLISAPAGYGKTTLLAQWSHAVEDST
                     SFAWVSLDEQDNDPVRLWRHILEALRRVAPEENFGADVLVGMSAAWQTLAATSLPMLI
                     NVLTDLPRRIVLVLDDYHCVTEDDSHESVAFFMEHLPENVHLVVASRSDPRLPLGRLR
                     ARGEMGEIRTEQLAFSEEEAATLLNEKMGLDIEPDDLLLLMNSTEGWPAGIYLASLTL
                     RHREDKHTFIESFGGSNRYVVDLLGEEVLARLPEDVREFLLTTSVLRSMTGSLCEEVT
                     GREDSGQVLRELARSNLFVVPLDEQGEWYRYHQLFSEFLHYELKSDRPELVPVLHGRA
                     SSWCESAGYLEGAIRHAIAAADHERTGRLIARYWHVYFFAGRTVTVERWLDTLPEDLI
                     YTDTALILVKAWIAATHGRGEERERYLALAESSSHEGRLPDGTASVESNVALIRAVFA
                     VGGVQSVVEAARRAAALEAEQSSPQAAWVRLGLGNGLYLSGDTSAARKPLEEALTLTK
                     AGPPLLRLAVLFMLSTVALDGGHLGEAESLARESHELVDRFRLSTIPQASLAAIALGR
                     VLAERGNLADAQVELEKGLSVRLRIPALSPWPTFLGLLALASVHRARGERAEARQAIA
                     QARAILEPFADDAGIFPELLERQERKLRMSKERNGHLDEELTEREGAVLRLLAGELAT
                     RQMADSLYVAPSTVRTQIKSIYRKFGVSSRDEAVAEARARGLI"
     gene            3185..3499
                     /locus_tag="VHM69_05955"
     CDS             3185..3499
                     /locus_tag="VHM69_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739971.1"
                     /translation="MKIDHPAVPYALRTLERGDTQRTRVGTRTVYRIVMCGELSPRYA
                     AAFEGMNMEARNGCTILTGEVIDQPHLHGILDHIGALGLEILSVESFSDEPYDNEARI
                     VG"
     gene            3546..3860
                     /locus_tag="VHM69_05960"
                     /pseudo
     CDS             3546..3860
                     /locus_tag="VHM69_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3966..>4387
                     /locus_tag="VHM69_05965"
     CDS             3966..>4387
                     /locus_tag="VHM69_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX2739972.1"
                     /translation="MCRWIAYSGSPILLEELLYKPDHSLIDQSLHARLGVETTNGDGF
                     GVGWYGANVDTPAVFHSVEPAWNERNLRELAGHVESPLFLAHIRASTGTAVQQTNCHP
                     FRYGRWLWVHNGLVRDFPRVKRELAIQVDESLYPYIEGS"
BASE COUNT          861 a         1361 c         1322 g          843 t
ORIGIN      
        1 cttgcgcccg gtgatgtctt cgtgcgagat cacggcccac cgcgcgtcgc caacgagaag
       61 gcgcgcaacc catccgacga accagcgtcg ttctccgggg gagtggcaag gatagtccat
      121 ggtgaactcc tggcgctggc ccgatagcac caaacgaacc ccctccgcga agcgagcagc
      181 ctcccgagcc ccgtcacccg tcgccgcttc gcatgcccgg aggtagttga cgccctccgt
      241 cgctatggcg gcgccgtcat tggtcgcggc gaactcatgc cacgccctgt tcacggcgac
      301 tatggctccg gactcgtcga tgattgccac tcgcgtcggt agattgtcca gcgccgcgcc
      361 gacgagaaac tcgcgctcgc ccatcgggcc cgccccgtcg gaggtcacac cgttaccctc
      421 acgcatcttt ccggctaatc gtctgcaaca gacgcctcct tcgacacgcc ggctgaagtg
      481 catttgttct tagatggtat ctaatcttac cgccgttcca tcctgtttgt attacatcac
      541 gagctttctc aacaacgaga cttggtcaac tccagcgttc ctgcccccca cagaagcgca
      601 acgggcacaa ttgtcaagaa acctttatca aaactttatc gtccgttgac caagacgagc
      661 cagtgacgga ctacattctt cctgaaagga gaaagagtaa gcgacgggcc acgtgatcca
      721 tggaagtggt ccatggtcgt catgcaaaag atgcgcaagc tgggcacagg tccttaccct
      781 gagcgtatga accggaaagt agcgcgatgt ctcgctggtc ggacgttgag cacgcgcaac
      841 tctagttagc ggcaccggac agaggagact gtaatccagg cttccagtga tccgaacgtc
      901 tatccttcga acatcgtcat caagcccaag ttgcgagcgc cgtcgccacg ctccgagcag
      961 ttggcccgac ccaggttgct cgatctcctc ggagaatcct tgcaccgcaa ggccaccctc
     1021 ataagcgccc cggccggcta cgggaagacg acgctcctgg cccagtggag tcacgcagtg
     1081 gaagatagca catctttcgc ctgggtctca ctagacgaac aggacaacga tccagtcagg
     1141 ctgtggaggc acatattaga agcgttgcgc cgggttgcac cagaagagaa cttcggagcc
     1201 gatgttcttg tgggaatgag tgccgcctgg cagacgctcg ctgcgacatc gcttcccatg
     1261 ctaataaacg tgctcacaga cttgccccgt cgcatagtac tggtgctcga tgactaccat
     1321 tgcgtaaccg aggacgactc tcatgaatcg gtggctttct tcatggagca tctccctgaa
     1381 aacgtccacc tcgtcgtcgc tagccgttct gacccacggt tgcctcttgg gcggttaagg
     1441 gcaagaggcg agatgggtga gatccgcacc gagcagctcg ccttctccga ggaggaggcc
     1501 gccaccctgc tgaatgagaa gatggggctg gacatcgaac ctgatgacct cctcctcctg
     1561 atgaacagca ccgagggttg gcccgcgggg atctaccttg cctccctcac tttgcggcac
     1621 agggaggata agcacacctt catcgaatcc ttcgggggca gcaaccgcta cgtcgtcgac
     1681 ttacttggag aggaggttct ggcacgtctc cccgaggatg tgagagagtt tctgctcacg
     1741 acctcggtgc tgaggagcat gacaggctct ctttgcgagg aagtgacggg tagggaggat
     1801 tcaggccagg tgctgcgtga gctcgcccgc tccaacctgt tcgtggttcc cctggacgag
     1861 cagggtgagt ggtatcgcta tcatcaactc ttctccgaat tcctgcacta cgagctcaag
     1921 agcgaccgtc ccgagctggt ccccgttttg cacgggcggg cgagctcctg gtgtgagagt
     1981 gcggggtact tagagggggc gatccggcac gccatcgcgg ccgcggacca tgagcgcacg
     2041 ggccggctca tcgcgcggta ctggcacgtt tacttcttcg ccggccggac ggtgaccgtg
     2101 gaacgatggc ttgacacgct acccgaagat ctcatctata ccgacacggc tctcatcttg
     2161 gtgaaggcat ggatcgccgc aacgcacggc cggggagagg agcgagaacg ctatttggcc
     2221 ctcgccgaga gtagctccca tgaagggagg cttcccgacg gaaccgcctc ggtcgagtca
     2281 aacgtggccc tcatacgggc cgtcttcgct gtcggcggcg tccagagtgt cgtcgaggcg
     2341 gctcggcggg ccgcggcatt ggaggccgag caatcctcgc cgcaggccgc gtgggtacgc
     2401 ctggggctgg ggaatggctt gtacctttcc ggagatacat cagccgctcg aaagccgctc
     2461 gaagaagccc ttactctgac taaggctggc ccgcctttgt tgcggctggc cgtgctgttc
     2521 atgctctcga ctgttgcctt agacggaggg cacctggggg aggccgagtc gcttgctcgc
     2581 gagtcgcacg agttggtgga cagattccgg ttgtccacga tacctcaagc tagtttggcg
     2641 gccatcgcac tcggtcgcgt gctcgcggaa cgcggaaacc tggctgatgc tcaggtcgaa
     2701 ttggagaagg gcctttcggt ccggctgcgg attcccgctt taagcccctg gcccaccttc
     2761 ctcgggttgc tggcgctcgc gtcggtgcac cgcgcacgcg gcgagcgggc cgaagcacgg
     2821 caggcgatcg cccaggcgcg agcgatcctg gagcccttcg ccgacgacgc cggtatcttc
     2881 cccgagcttc tcgagcgcca ggagcgcaag cttcgcatga gcaaggagcg caatgggcat
     2941 ctcgacgagg aactcacgga gcgcgagggg gctgtgcttc gcttactcgc cggcgaactc
     3001 gccaccaggc agatggcgga cagcctctac gtcgccccca gtacggtaag aacccagatc
     3061 aaatccatct accgcaagtt cggggtttcc tcacgtgatg aagccgtggc ggaagctcgc
     3121 gccagagggc tcatctaggc ttcccacaga atccctaaat cccctagggg aaatgttgca
     3181 ttgggtgaag atagaccatc ctgctgtgcc atatgctctg aggacattgg agcgcggcga
     3241 cacacagagg acccgtgtgg gcacgaggac ggtctaccgg atagtgatgt gcggggagct
     3301 tagcccgcgc tatgcggcag cctttgaagg gatgaatatg gaggcgagaa acggatgtac
     3361 gatcctcaca ggggaggtca tcgaccagcc ccaccttcac ggcatcctcg accacatagg
     3421 agctttgggg cttgaaatac tgagcgttga gtccttttcc gatgaaccct acgacaacga
     3481 agcgagaatc gtagggtagc aagcacgtac gggcgaaggc tacggtccgt cgtaggcgga
     3541 acagggtcag acaggaggtc aacacgatgg gttgtctatt ggccatcttc gctgggttct
     3601 tcccacgcct cgcgctgttc ctcctctgga tcgccaggcc ggccatcgtc ggtgcggcgt
     3661 tcaacacgtt ccttatcccg ttgctaggca tcatcttcct ccctttcgcg accctgatct
     3721 acgtgctctt ctatacaccc ggcgtcgggc tgaccggctg ggagtggctc tgggtgatca
     3781 tcgccgtgct gctcgacatc ggtcactggg gggccaccta cacccagcgc aaccagatac
     3841 cgggcacacg gggcgcgtga acgcagtagc gcacagcacg gatctcggcg agcggatcgt
     3901 cgatgccacc gcagcgatga tgcggcagta gccacggtcg acacacggac ggaaaggagt
     3961 agaagatgtg ccgctggata gcctactctg gctccccgat cctactagag gagcttctgt
     4021 acaagccgga tcactccctc atagaccaga gcctccacgc ccgcctgggt gtagagacga
     4081 ccaacgggga tggcttcggc gtcggctggt acggcgcaaa cgtcgacaca ccagcggtct
     4141 tccacagcgt cgagcctgcc tggaacgagc gcaacctgcg cgagcttgcc ggccacgtcg
     4201 agtcacccct gttcctcgcc catatccgcg cctcgacggg tacggccgtc cagcagacta
     4261 actgccaccc cttccgctac ggcaggtggc tatgggtaca caatggcctc gtgcgcgact
     4321 tcccgcgggt caagcgagag ctcgcaatcc aggtagatga gtcgctctat ccatacatcg
     4381 aaggatc
//
LOCUS       DATAIR010000249         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_330013, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000249 DATAIR010000000
VERSION     DATAIR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330013"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VHM69_05970"
     CDS             <1..1971
                     /locus_tag="VHM69_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX2739973.1"
                     /translation="HHYPVQDVPHEFMSLQGGFRRDLEQAKSVVRGWLEEADAARRTE
                     EPASELKIGLQCGGSDAFSGVSANPLVAWVSGEVVKNGGIANLAETDELIGAEHYVLK
                     NVRELATARRFLSTVERFKERVSWHGHTAEDNPSGGNNYRGLYNISVKSIGAAMKKHP
                     DVRIDRVIEYAERMDEGGFYFMDSPGNDLESVAGQVASGANMIFFTTGNGSITNFPFV
                     PTIKFVTTTGRYELLSKDMDVNAGAYLEGTPMEELGRETFERTLRAASGERTVGERAG
                     HSQVSIWRDWKQTSDENLALLRNAPEPDGEPLAVRKAVPDVEFSFEAIRSGRGPVVDQ
                     VGLVMPTSLCSGQISRRIANRLNERDATQGRVTRFVALPHTEGCGVSAGSAETIYSRT
                     VLGHLASPTVRFGLLLEHGCEKTHNDYFRNRLEEAGVDPARFGWASVQLDGGIDSVVE
                     KVEDWFADTLESAEELEYEHAGLESLRLGLYAAGPISDEAALSLAQITLAVVGSGGTV
                     IVPERAAVLSSPVYLDAVLGGRSVENTLSYGQDATKPGLHVMEAPTDHWVETTTGLGA
                     TGVEVMRTHVAGHPLQAHRMIPLIQASSNPETLAKHAEDLDVILEGDPSEWAGQMLET
                     VSAVASREYVPKLFEAGNTDFQFTRGLLGVSM"
     gene            complement(2032..>2512)
                     /locus_tag="VHM69_05975"
     CDS             complement(2032..>2512)
                     /locus_tag="VHM69_05975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX2739974.1"
                     /translation="HDVGLVILDQAIRLPEYGVLATAGSLDRLAAQQAKREVIFTSSG
                     YGLSESNPVHVTSFRERLMAGSRLTNLGNALTDGYNLQTNGNGSDRGGTCSGDSGGPV
                     FYGGFTSNTIVAVTSFGQNAYCRGVDFSYRTDTQEVIDWILATVPEREADKIQFVTL"
BASE COUNT          465 a          777 c          837 g          433 t
ORIGIN      
        1 caccactatc ccgttcagga cgtgccgcac gagttcatga gcctgcaggg tgggttcagg
       61 agggatctcg aacaggctaa atccgtcgtg cgcggatggc tcgaggaggc ggacgcggcg
      121 cggcgcaccg aggaaccggc ctccgagctc aagatagggc tccagtgcgg agggtccgac
      181 gccttctcgg gggtctccgc gaacccgctc gtggcgtggg tgagcgggga ggtcgtcaag
      241 aacggcggca tcgccaatct ggccgagacg gacgagttga tcggggccga gcactacgtc
      301 ttgaagaatg taagggagct cgcaaccgcg cggcgcttcc tctctacggt cgagcgcttc
      361 aaggagcgcg tctcgtggca cggacacacg gccgaggaca atccgtccgg gggcaacaac
      421 taccgcgggc tctacaacat ctccgtcaag tccatcgggg cggcgatgaa gaagcaccct
      481 gacgtacgga tcgaccgtgt gatcgagtac gccgagagga tggacgaggg cggcttctac
      541 ttcatggaca gcccgggaaa cgacctcgag agcgtcgcag ggcaggtcgc ttcgggtgcg
      601 aatatgatct tcttcaccac gggcaacggc tccatcacca acttcccctt cgtcccaacg
      661 ataaagttcg tcaccaccac aggccgctac gaactgctct cgaaagacat ggacgtgaac
      721 gcaggcgcct acctcgaagg cacgccgatg gaagagcttg gccgcgagac tttcgaacgg
      781 acgctcaggg cggcttcggg cgagaggacc gtcggggagc gcgcgggcca ctcccaggtc
      841 tccatctggc gcgactggaa gcagacgagc gacgagaacc tggcgttgct caggaacgcg
      901 cccgagcccg atggcgagcc gctagccgta aggaaggcag ttcctgatgt cgagttctca
      961 ttcgaggcaa taaggagcgg gcgggggcct gtcgtggatc aggtcgggct cgtcatgccg
     1021 acgagcctgt gctcggggca gatctcacgc cgcatagcga accgcctgaa cgaacgcgac
     1081 gccacgcagg gcagggtaac ccgcttcgtc gccctgcctc ataccgaggg ctgcggcgtc
     1141 tccgccggct cggctgaaac gatctactcc cgcacggtac tgggtcatct cgccagcccc
     1201 accgttaggt tcggcctgct actcgaacac ggctgcgaga agacccacaa cgactacttc
     1261 cgcaaccgtc tcgaagaggc cggcgtcgac cctgcccgct tcggctgggc gagcgtccag
     1321 ctcgacggcg gcatagacag cgtggtagag aaggtagagg actggttcgc cgataccctg
     1381 gagagcgccg aagagctgga gtacgaacac gccggccttg agtctctgcg gcttggtctt
     1441 tacgctgccg gtcccatatc cgacgaggcc gccctgagcc tcgcgcagat aacgctggcc
     1501 gtcgtgggct cgggcggcac tgtgatcgtc ccggagaggg ccgcggttct ctcttcaccg
     1561 gtctacctcg atgctgtgct cggcggccgt tccgtagaga acaccctgtc ttacgggcag
     1621 gacgcgacga agccggggct tcacgtcatg gaggcgccga cggaccactg ggtcgagact
     1681 actacggggc tcggggcgac gggtgttgag gtgatgcgga cgcacgtcgc cgggcatcct
     1741 ttgcaggcgc accgtatgat cccactaatt caggcttcct ccaaccccga gacgctggcg
     1801 aagcacgccg aagacctcga cgtgatcctg gaaggggatc catccgaatg ggccggacag
     1861 atgcttgaga cggtctcggc cgtggcctcg cgcgagtacg tccctaaact gttcgaggcc
     1921 ggcaacaccg acttccagtt cacacgcggt ctgctcggcg tctcgatgta gcctggcaga
     1981 gtagagggcc ggtgagaagc cggccctcca catagttgcg ttcctctggg gttagagggt
     2041 gacgaattgg atcttgtccg cctcgcgttc gggtacggtc gcgaggatcc agtcgatcac
     2101 ctcttgagta tccgtcctgt acgagaagtc cacgccccgg cagtaggcgt tttggccgaa
     2161 ggatgtcacg gcgactatgg tgttcgaggt gaatccgccg tagaagacgg ggccacccga
     2221 gtctccggag caggtgccgc cgcggtcact accgttgccg tttgtctgga ggttataacc
     2281 atccgtcagc gcgttcccca ggttcgtgag cctcgacccg gccatgagcc gttcgcggaa
     2341 agacgtcacg tgcacggggt tgctctcgga gagcccgtag ccgctggagg tgaagatgac
     2401 ctcccgcttt gcttgctgcg cggccagcct gtcgagcgag cctgccgtgg cgagcacacc
     2461 gtactcgggc agtctgatgg cctggtcgag gatgacgagg ccgacgtcgt gc
//
LOCUS       DATAIR010000250         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_107915, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000250 DATAIR010000000
VERSION     DATAIR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107915"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1026
                     /locus_tag="VHM69_05980"
     CDS             142..1026
                     /locus_tag="VHM69_05980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2739975.1"
                     /translation="MESVGFIGLGAMGAPMAGRIVAAGFDLSVFDVRPENAEPLVEAG
                     AARAGSPREVADGVDALVLMVVNAGQAEDALFGSEGAIEALSAGSAVVIMSTVGPEAV
                     RALEGRLAERGVRLLDAPVSGGVSRAERGDLLIMAGGTADLFEELRPVLQAMGSTVAH
                     CGGSGGDGQSVKLVNQLLCGVHIAAAAEALAYAEAMGLDPRSVFETVRHGAANSFMLE
                     DRGERILEREFVPAKSALDIFVKDMGLVREAAGEQGFTTPLADAAHRLYEVGSSLGLG
                     GEDDSGVVRVFERTDDAV"
     gene            1222..1578
                     /locus_tag="VHM69_05985"
     CDS             1222..1578
                     /locus_tag="VHM69_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2739976.1"
                     /translation="MKRVMIVEDHSAFAQALELVLGKVDGTEVALARTLEEGRGLIQN
                     GGPFDLVVLDLALPDGEGSDLVPELRQLHPKTPIAVLSARDDIAEVASKAGADAAIPK
                     ETPLPEIISSFRRLAG"
     gene            1655..1960
                     /locus_tag="VHM69_05990"
     CDS             1655..1960
                     /locus_tag="VHM69_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2739977.1"
                     /translation="MDLVRLDPSSGFEVLAGTERSQAATMVLAPGTSTGGPDNRHPES
                     DQWVYVISGDGSAVVEGTERELGAGSLLLIEAGEAHEIKSAGDGPLQTLSIYAPAVY"
     gene            complement(1974..2447)
                     /locus_tag="VHM69_05995"
     CDS             complement(1974..2447)
                     /locus_tag="VHM69_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739978.1"
                     /translation="MGYVDLRRRREERKPGGLGYYALNNPHLLLATGFVLGLLSSPVF
                     YIGVNSPDPFAALLLLIAAVTLLGYVSWLLLGQGSKIIRPRVGGEKQLLMAILEAGGS
                     ITPVEAALQTSLTVDEAEEILTRFADRGHLLVESRNGALFYALPGRRLPSETWTH"
     gene            2542..3297
                     /locus_tag="VHM69_06000"
     CDS             2542..3297
                     /locus_tag="VHM69_06000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX2739979.1"
                     /translation="MAFSEEVAWVTGSSTGIGRAVAVALAEGGRRVVVHYNASEDEAR
                     EVVEDIESSGGEASLVGGDVSDAGEVGRMVGEIEDRYGRLDVLVNNAGSLIERRTLEE
                     MTEDLWDRVMDVNLKSVYLVSRAVLPMMKRKGRGRIINLTSIAARNGGGPGSVAYATA
                     KGGVSTLTRAMAKELISENILVNGVAPGVITTPFHDRFTPPEVRESLKSAIPMGREGT
                     PEETAGVVAFLASPAADYLVGEIIEVNGGQLMS"
BASE COUNT          708 a         1097 c         1308 g          707 t
ORIGIN      
        1 cagatgcttc ccggcataat atccgtctgg atcctgcccg acgacagcgc ctttcccggc
       61 ctgccctacg tcatcttcgc cggcaacgtg ggcgaggagg actcgcttgc cagggtcata
      121 gagatcctca gggagggata gatggaaagc gtcgggttta taggactcgg ggcgatgggc
      181 gccccgatgg cagggcggat cgttgccgcc ggtttcgacc tctcggtctt cgacgtacgc
      241 cccgagaatg cggagccgtt ggtcgaggcc ggggccgcac gcgcgggctc accgcgggaa
      301 gtggctgacg gagtagacgc gctagtcctt atggtcgtga acgcagggca ggctgaggat
      361 gcgctgttcg gaagcgaggg cgccatcgaa gccctgtcgg cgggatcagc cgtggtgatc
      421 atgagcactg tcgggcccga ggcggtccgc gctctcgaag ggcggttggc cgagcgtggg
      481 gtacggttgc tcgacgcgcc tgtcagcggt ggggtgtcgc gggccgagcg gggagacctg
      541 ctcatcatgg ccggcggtac tgctgatctg ttcgaggagc ttcggcccgt gctgcaggcg
      601 atggggtcca cggtcgcgca ttgcgggggg tcggggggcg acgggcagtc cgtcaagcta
      661 gtcaaccagt tgctgtgcgg ggtgcacatc gctgccgcgg cagaggcgct cgcttacgcg
      721 gaggcgatgg ggctcgatcc ccggtcggtg ttcgagacgg tcaggcacgg agcggccaac
      781 tccttcatgc tcgaggacag aggcgagcgg atcctcgagc gtgagttcgt ccccgcgaag
      841 agcgccctcg acatcttcgt caaggacatg gggctggtgc gcgaggccgc gggtgagcag
      901 ggtttcacga cgccgctcgc agacgccgcg caccgactct acgaggtggg atcctcgctc
      961 ggcctaggtg gggaggacga ctcgggtgtc gtacgcgtct tcgagaggac ggacgacgct
     1021 gtctgaggct cgacttcgtg gtcgaggcgg acctcaatgc gcgggaggac atccacatcc
     1081 gggattcgcc ggtttccgga agtgtcatcg tctgctatcc tccagatctg gttcatttgc
     1141 acgatttacg ttatgctcgt tctccgttag ggttgtgctc gatgggcttg tcaggcaggg
     1201 aaatggcgcg gaggtgtcgc gatgaagcgc gtgatgatcg tagaggacca ctcggccttc
     1261 gcgcaggccc ttgagctcgt tctcggcaag gtggacggta ctgaggtcgc cctggcccgc
     1321 acgctggagg agggacgggg actgatccag aacggcgggc ctttcgacct cgtggtcctc
     1381 gacctggcgc tccctgacgg ggaagggtcg gatctcgtcc ccgagctcag gcaacttcac
     1441 ccgaagacgc cgatcgccgt cctcagcgcc agggacgaca tcgcggaggt cgcctcgaag
     1501 gccggtgccg atgccgcgat tcccaaggag acgcccctgc ccgaaataat ctcctccttc
     1561 agacgcctcg ccgggtagac gagcagcaaa ccgtgtcgcc actgattgtg gtaccgtttt
     1621 gcgtgtattg ggtccgtatt cagggaggtt cagcgtggat ctcgtacggt tagatccctc
     1681 gtccggattc gaagtcctgg cagggacgga acgctcgcag gccgcgacga tggtgctcgc
     1741 accaggcacg tccacgggag ggccggacaa caggcaccct gagagcgacc agtgggttta
     1801 cgtgatctcc ggcgatggca gcgcggtcgt ggagggcacg gaacgcgagt tgggcgcggg
     1861 gtctctcctg ctcatcgagg caggagaggc gcacgagatc aaaagcgcag gggacggtcc
     1921 tctgcaaacg ttgagcatct atgcacctgc ggtttactag attcggtcag gctctaatgc
     1981 gtccacgtct cggaaggcag acgcctgcct ggtagtgcgt agaagagggc gccgtttcgg
     2041 ctctcgacca ggagatggcc gcggtcggcg aagcgggtga ggatctcctc ggcctcgtcc
     2101 accgtgagcg aggtctggag cgcggcctcc accggcgtga tgctaccgcc cgcctccagg
     2161 atcgccatga gcaactgttt ctctccaccg actcttggcc tgataatttt ggaaccctgt
     2221 ccgagcagaa gccagctcac ataaccgagc agcgtgaccg cggcgatcaa aagcagcaag
     2281 gcggcgaaag gatcagggct gttgacgccg atgtagaaga cgggcgaact gaggaggcca
     2341 aggacgaacc cggtcgccag gagcaggtgt ggattgttta gcgcgtagta cccgaggcca
     2401 ccaggcttgc gctcttcgcg tcgtctccga aggtccacgt agcccacgtc gcgcctcctg
     2461 cgttgttttc cccgaccgga accattatgt taccggagca tcaattgtag aggagaaagg
     2521 ttacagggag gtaacggtcg catggctttc tcggaagagg tcgcgtgggt gacagggagc
     2581 agcacgggga tcgggcgcgc cgtagccgtg gcgctcgcgg aagggggacg ccgggtggtg
     2641 gtccactaca acgcgagcga ggacgaggcg cgggaggtgg tagaggacat cgagtcctcc
     2701 ggcggagagg cttcgctcgt cgggggcgac gtctcggatg ctggcgaggt cgggcggatg
     2761 gtcggggaga tcgaggaccg ctatggaagg ctcgacgtgc tggtcaacaa cgccgggtcc
     2821 ctgatcgaac gccggacgct cgaagagatg acagaggacc tgtgggaccg cgtgatggac
     2881 gtgaacctga agagtgtcta cctggtctcg cgggccgtct tgccgatgat gaagcggaag
     2941 ggtcgaggca gaataatcaa cttgacctcg atcgctgccc gcaacggcgg agggcctggc
     3001 tcggtcgcct acgccacggc caagggcggt gtcagcaccc taacccgcgc gatggcgaag
     3061 gagcttatat ctgagaacat cctggtaaac ggggtcgccc ccggcgtcat aaccacgcct
     3121 ttccacgacc gcttcacgcc tcccgaagtg cgcgagagcc tgaagagcgc catccccatg
     3181 ggacgcgagg gcacccccga ggagaccgca ggggtcgtcg cgttcctcgc ttctccggct
     3241 gcggactatc tggtcggtga gatcatcgag gtcaacggcg gccagttgat gagctaacca
     3301 cacaaaggtg tctcaagcgt atgtttccga tccggagccg ctgccagcca acgaccaccc
     3361 gacatggcta cctggctggg catgtcctgg cgggcctctt gtaccgagag ttcgcactcc
     3421 aagccgcacc gaaagcgatg agatgcctgc atgtttattc gacctgcgaa catcatgtgt
     3481 tcggtcatcc ggtgccgagg gcgcgctggc gccgcttact gaaccattgc aaggaattta
     3541 tgcaggatgc tgaaaaggga tttctgagat ttcttcattc cgcgcactag ggtgaataag
     3601 ccagccatgc atctagcgcc ccacttgtaa accgctttca accgtactat gcaacaaaaa
     3661 tcactcattt gggggaaaca tccctcttcg gattccacta ccatagtagc aagcactcta
     3721 gtggggagtg taagaaagga ggtagcgctc atgaagcgct tgatcatact atcgacgttg
     3781 gttgccacca gccttgtcat actgacattg gcaggtccgg
//
LOCUS       DATAIR010000251         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_330129, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000251 DATAIR010000000
VERSION     DATAIR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330129"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1570
                     /locus_tag="VHM69_06005"
     CDS             353..1570
                     /locus_tag="VHM69_06005"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2739980.1"
                     /translation="MTNDHRRPDAQMAQGILLDDPDFLREIVERVLQELMEAEMTQHV
                     GAAPHERTEARKGHRNGYKPRTLRTRVGTLNLLVPQDREGTFSTGLFSRYQRNEKALV
                     LALMEMYVEGVSTRKVKDVTEELCGTSFSKSLVSSLAGSLDSELSAWRDRRLEAKAYP
                     YLFVDARYEKARVDSRVVSQGVLVASGVRDDGMREILALEVADTESEATYHELFRSLK
                     ARGLRGVELVVSDDHEGLKSAVERHFQGASWQRCQVHYARNLLGMVGHGRRKELAEGL
                     RGVFAAPSREVALGLASELATRWRQSHPGVADHLEEHIEECLSCLPFPESHRRRIRTT
                     NGLERLNQEIKRRTRVVRIFPNRRACLRLVTALAVEQSEEWLTGRRYLDMRELQGRRQ
                     GEHDTEGVALMER"
     gene            1804..2475
                     /locus_tag="VHM69_06010"
     CDS             1804..2475
                     /locus_tag="VHM69_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2739981.1"
                     /translation="MTEARRTRVLLADDHTMFREGLARLLATYGGLEVVGETSNDEEA
                     IILAQRTRPDVIIMQVQMPFEKAQDSLRRMREILPEPKIVIVTMFENPQMMRGLLKLG
                     ASAYVLKSSSSRHLISAVRAAVFDPNSNNMVVGMPLEALEESDDGSGGILSARQLEIL
                     LLAARGLSNHQISTSLHIAEGTVKRHLADVYKKMKVRSRSEATRKALRDEWITIMDVT
                     GTDEG"
     gene            complement(2508..>2691)
                     /locus_tag="VHM69_06015"
     CDS             complement(2508..>2691)
                     /locus_tag="VHM69_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739982.1"
                     /translation="GADMRLHTGTLCWIEILYSDGLNARISLDGEVWEIEDISIDDLL
                     EIVERVQRSIPEGASE"
BASE COUNT          614 a          768 c          841 g          468 t
ORIGIN      
        1 cgaagcgaca gatgagtatg caactttgct tgaagggctt ctcaacatct catgattgtc
       61 aatacgcgtg ttgagagccc ttattagatg gatgaaaaga tatggtcagt cgactaaact
      121 gaggactgct ttgacacgac tagccagtca atactttccg acataaacgc cgctgtacaa
      181 ggcttcggca acatacagcc tggtttgcga accaagcata cagggacagc gacggttgag
      241 gtactccggt tgtgcccgta aagttctgta aattggtgag ccgctagaaa gagaggccat
      301 cgtcctccaa gatggttggt gtcttatgtc aaccgatcga aacggagtga cgatgaccaa
      361 cgatcatcgt aggccggatg cacagatggc gcaagggatc ttgctggacg acccggactt
      421 cttgcgcgag atcgtcgagc gggtgcttca ggagctgatg gaagccgaga tgacacagca
      481 cgtaggcgcc gctccccacg agcgcaccga agcccgcaag ggccatcgca acggatacaa
      541 gcccaggacg cttcgtacca gggtgggcac cctaaacctg ctcgtacccc aggacagaga
      601 ggggacgttc tccaccgggc tgttctcacg ttaccagcga aacgagaagg cgctcgtcct
      661 ggccctgatg gagatgtacg tggagggggt ctcaacaagg aaggtcaagg acgtgaccga
      721 agagctgtgc ggcacctcct tctccaagag tctcgtctct tctttggccg gctctctgga
      781 ctcggagctt tcggcgtgga gggatcgccg gctagaggcg aaggcttacc cctacctgtt
      841 cgtggatgcc cgctacgaga aggcgagggt ggactctcgg gtggtgagcc agggcgtcct
      901 ggttgcctcg ggcgtgaggg acgatggcat gcgggagatc ctcgccttgg aggtcgcgga
      961 caccgagagc gaggcaacct accacgagct cttccgctcc ctgaaggctc gaggcttgcg
     1021 cggggtcgag ctggttgtct ccgacgacca cgagggccta aagagcgccg tggagcgtca
     1081 cttccagggg gcaagctggc aacgctgcca ggtccactac gcgcgtaacc tcctcgggat
     1141 ggtgggccat ggcaggcgca aggagctcgc cgaaggcctg cgcggggtgt tcgccgcgcc
     1201 gagcagggaa gtggcgctcg ggctcgcaag cgagctggcg acccggtggc gacagagcca
     1261 ccccggggtc gccgatcacc tcgaagagca catcgaggag tgcctctcgt gcctgccctt
     1321 ccccgagagc cacagaaggc gcatccgcac caccaacgga ctcgaacgcc tcaaccagga
     1381 gataaagcgc agaacgcggg tggtgaggat cttccccaac cgccgggcgt gcctacgact
     1441 ggtcacggcg ttggcggtcg agcaatccga ggaatggctg acggggagac gctacctgga
     1501 catgcgcgaa ctccaagggc ggcgccaagg agagcacgat acggaggggg tggcgctcat
     1561 ggagcggtag aggagggata gggcttggag gaaattacag aaaattcggg acttgaccta
     1621 ggctggatct ccctgcctga cccaaaatgt acaaaaatat gtagtataag gtacatagca
     1681 atacgtaaga tgtgacgcgg gtttgagttt ggattacact cacgtgctcc aatgaagcag
     1741 gcaagccgcg tcgaaagaag cgggaagcga ggaaaccgtg cagccacaaa ctcctcacaa
     1801 cctatgacag aagcccgccg tacgagggtg ctactcgccg acgaccacac catgttccgc
     1861 gaagggctgg cgcgcctgct cgccacctac gggggacttg aggtggtcgg ggagacatcc
     1921 aacgacgaag aggccatcat actggcccaa cgcaccaggc cggacgtgat catcatgcag
     1981 gtgcagatgc ctttcgaaaa ggcgcaagat tcgctgcggc ggatgcgtga gatcttgccc
     2041 gagcccaaaa tcgtaatagt gaccatgttc gagaacccgc agatgatgcg tgggctactg
     2101 aagctagggg ccagcgccta cgttctcaag agtagctcct ccaggcatct catcagcgcg
     2161 gtgcgggccg cggtgttcga ccccaactcg aacaacatgg tcgtcgggat gccgctggag
     2221 gccctggagg agtcggatga cgggtcggga ggcatactct cggccaggca actggagatc
     2281 ctgcttctgg ccgccagggg cctctccaac caccagatat ccaccagcct gcacatcgcc
     2341 gaagggaccg tcaaacgtca cctcgcagac gtgtacaaga aaatgaaggt ccgttcacgg
     2401 agcgaggcca ccaggaaggc cctgcgggac gagtggatca ccataatgga cgttaccgga
     2461 acggacgaag ggtagacgga aaaccaggcc ggacaacggc taacaagcta ctcggacgcg
     2521 ccttccggga tcgatctctg tacccgctcc actatctcta acaggtcatc tatgctgata
     2581 tcttctatct cccacacctc gccatcgagg ctgatcctgg cgttcaagcc gtcgctgtag
     2641 agtatctcga tccagcacag ggtgcccgta tgcaacctca tgtccgcccc g
//
LOCUS       DATAIR010000252        11934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_384973, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000252 DATAIR010000000
VERSION     DATAIR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384973"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1873
                     /locus_tag="VHM69_06020"
     CDS             293..1873
                     /locus_tag="VHM69_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2739983.1"
                     /translation="MDAYKSLSRADQTTPLGGEELGLLATSAFMVGRDDDFLGYLERA
                     HHAYMDAGETMRAVRCAFWMGINFALRGEMSRATGWLGRAHRLVEREEGDCVERGYLL
                     VPVMLRHEANGNWDAAYTAAAEAAEIAERFSDADLLALALHEQGLILVNQGRVEEGLG
                     LVDEAMVAVASGELSPIVTGIVYCSVISGCQEVYALRRAQEWTAALTQWCEEQPEMVA
                     FSGQCLVHRAEIMQLHGAWLDALDEALRARKRCEQGKNRMATAQAFYRQGEVHRLQGN
                     YAAAGDAYRDASRYGWEPQPGLALMWFAEGKGDAACAALRRALGETTKPLKRARLLPA
                     YIEIMLAVGDVEEARSACREFGEISARYESGMLDAMVAHAKGAVDLTEGDARDSLLAL
                     RRALQLWQELEVPYEAARARVLLGLACSALGDHDTAELELEAARSSFSDLGAAPDLAR
                     VDSLSRRAPSGDIHGLTPRELQVLRLVAAGETNKAIAAGLVLSERTVDRHVSNIFAKL
                     GVSSRTAATAYAYKHQLV"
     gene            1997..2539
                     /locus_tag="VHM69_06025"
     CDS             1997..2539
                     /locus_tag="VHM69_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX2739984.1"
                     /translation="MTFTLDALSLQVLPPEMALREAVPILYRLVGDPTFLDSHVLAHL
                     EEAGRAEDWYVAFRDDRSCSLQVFVWPPGSRTQIHDHSTWGAFCCVVGTVLEERFERA
                     DDCSLTDFARLKKLWRLEWRRKDGISTVLPYERGIHRVGNPTGEPAISVHLYGPRLGE
                     IDGRDYDPSQNYVCDRPEDR"
     gene            2751..3902
                     /locus_tag="VHM69_06030"
     CDS             2751..3902
                     /locus_tag="VHM69_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2739985.1"
                     /translation="MSKHRYVKDHDRHLADSTEYVETVIVGGGQAGLSVGYHLARRDR
                     PFVILDANERVGDAWRKRWDSLRLFTPARYDGLEGLPFPAPAVTFPTKDEMADYLESY
                     AAHFDLPVRTGVKVDGLSRQDGRYALTSGDRRFEAEHVVVAAGAHQIPKIPAFANELD
                     SSILQLHSSQYRHPSQLHEGTVLVVGAGNSGAEIAFEVSSTHPTYLSGKPSGQLPVRH
                     GPAMARFVFPVVRFVGHHVLTLRTPIGRKIQPKFISHSAPLIRIKLKDLAVAGVEQVP
                     RTVGIEGGRPALEEGRVLDVSNVLWCTGFREVFPWIDLPIFGEDGRPLHERGVVVGEP
                     GLYFVGLVFQYAVSSDVLPGVGRDAEYIAKYIASREPKGRSRSHALARA"
     gene            4018..4629
                     /locus_tag="VHM69_06035"
     CDS             4018..4629
                     /locus_tag="VHM69_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739986.1"
                     /translation="MSQKILWVGRTTIFLVGLAVMLAVVLGLASAALAGTGVGDVFNL
                     GKKNTVGQLSQLVGRSDSAMLKVENNSAGGDATALNLQVEPGNAPMKVNSSTRVSNLN
                     SDQLDGKDASTFMPASIYTVDETVEMGSNVTGTVRVVFCDEGDVPISGGFSGLGSQTQ
                     IKQSSRVFYVGRPTWWVAANKPVGPEDTWTAQAVCADFGEMHK"
     gene            4778..5242
                     /locus_tag="VHM69_06040"
     CDS             4778..5242
                     /locus_tag="VHM69_06040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2739987.1"
                     /translation="MEESKEKTVERESPEVRNRYPHFLTVPTRWMDNDVYGHVNNVVY
                     YSYFDTVVNEYLISTGVLDIETSPVIGLVVETQCRFFKPITFPDVVHAGLRVARLGRS
                     SVRYEIGLFRNEDDGAAAQGHFVHVYVERESRKATELPPGMRAALEKILVSC"
     gene            complement(5239..5727)
                     /locus_tag="VHM69_06045"
     CDS             complement(5239..5727)
                     /locus_tag="VHM69_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2739988.1"
                     /translation="MSEKTGLREDLKTEVEDLVASDAPDDEVLNEVVRRVHEAHPSWD
                     WSGIYLLVGDTLVLGPRTAPADHSLIGIGEGVCGTAVSEDRNQIVEDVREVENYLACS
                     IHTRSELVVLIRDAGKVVGEFDIDSDTVGAFTQVDEAMLEETAALISGRVASLAASYE
                     SS"
     gene            6093..6569
                     /locus_tag="VHM69_06050"
     CDS             6093..6569
                     /locus_tag="VHM69_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2739989.1"
                     /translation="MRRVQMTVDGRAHVAEVEPRLLLLHHLRENLGIGGVHSGCNTTN
                     CGACTVHIDGESVKSCTVLTVQADGHEITTVRGIMDNGLWHPMQQAFHEAHGLQCGYC
                     TPGMIMASISLLKENPDATEQEIRDGLEGNLCRCTGYENIVKAVQLVQQHTNGAAS"
     gene            6583..9156
                     /locus_tag="VHM69_06055"
     CDS             6583..9156
                     /locus_tag="VHM69_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2739990.1"
                     /translation="MTEAPEKFVGGGVPRKEDPALVTGRANWTDNIKLPGMLHATLMR
                     SPFAHAKITSIDVSAAREQPGVVAVFTGDDLADDWDKNPQANPASVTGYGISLAERAQ
                     GASSATDTADTTDGQPADEEPKTFVSWIPTDDTKIPNHWPVARDEVNYAGDIVAVVVA
                     TDRYKAQDALEFIEVDYDPMTPVLDVEEAVAEGSPLVHEDIGTNESFTWTLGTGDIDD
                     AFNRADVVVKERYLQQRLIPNAIETRGVVANPDPVTGGYTLYSSTQIPHILKYCLAVY
                     CGVPEQQLRVVAPDVGGGFGSKLNVYAEEAIALVLARKLGVPVKWIEDRSENYLATIH
                     GRGQVQDIELAATSEGKILGMRVNLLADMGAYLMLDGPGIPILGAFVYPGVYTFGAYD
                     FSVTGVFTNMTPTDAYRGAGRPEAIFAIERIIEALAREVGLDPLEVRRRNFYEPFDEP
                     TDTPAGIQYDSMNMQPALDRVLELADYEGLREEQRRRRENNDPVQLGIGFSTYTEICG
                     LAPSQVLAGLRTGGSGWETAKVQMLASGKVEVVTGTSPHGQGHVTSWSQIAADALGVD
                     VNDVEILHGDTALSPYGRDTYGSRSLPVGGVAVHLAAQDVVNKARKIAAHMLEAAEGD
                     MEFEGGKFSVAGSPDQSVTIQEVATAAYMNYDLPAGMEPVLSEEVFWDPPNFTFPFGA
                     HVCVTEVDTETGFVKIRDYFGVDDCGPVINPTIVDGQLHGGIAQGVAQALYEEAVYDE
                     EGNLTTGSMVDYLIPGAPELPNYTLERTVTPSPTNVMGVKGVGEAGTIGSPPAVINSV
                     VDALSPFGITHIDMPASPWKVWQAIQDAQGQDAGTRDADLSGRPSQAPDSTDTEGGST
                     S"
     gene            9153..10001
                     /locus_tag="VHM69_06060"
     CDS             9153..10001
                     /locus_tag="VHM69_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX2739991.1"
                     /translation="MIPIAFDYEVAESVDHAIELLGQHGDEAKLLAGGHSLLPLMKLR
                     LAAPTVLVDLGRIGDLNYVRDEGDHLAIGAMTRHCDMERDATLKEHCGLISYTASLVG
                     DPQVRHRGTIGGSVSHGDAASDLPSALLALDAIFVIKGPGGERTVAAGDFFEDYLQTT
                     LAPDEVLTEIRVSKLGGAGWSYKKFNRRAQDWAVVGVAAVVERSNGTIGSARIGLTNM
                     GSTPVRATAAENALAGADPASVAEATGSADEGTSPSSDIAASAEYRRHLARVLSRRAV
                     EEALGR"
     gene            10007..10942
                     /locus_tag="VHM69_06065"
     CDS             10007..10942
                     /locus_tag="VHM69_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2739992.1"
                     /translation="MSSERRGALPPGVSHFRELGEQLREHDYLSDEGLSTAIFLSLKL
                     EKPLFLEGEAGVGKTEVAKVLSRILDTPLIRLQCYEGIDVYQAVYEWDYARQLLHIRA
                     AEAFGGEDRERVERDLYDREFLVSRPLLQALEHRGPMPPVLLIDEVDRSDDEFEAFLL
                     EILSDFAVTIPEIGTIRAERPPVAVLTSNRTREVHDALKRRCLYHYIEHPSFEREVEI
                     LRLRVPDAEERLAREVAAATEKMRAMDLYKPPGVAETLDWTEALVALGARELDEQLVE
                     ATLGSVLKYREDQQRAANAGLAALIGGVRVVGNDA"
     gene            10935..>11934
                     /locus_tag="VHM69_06070"
     CDS             10935..>11934
                     /locus_tag="VHM69_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2739993.1"
                     /translation="MPEGSTPPMLDAAVAFGRVLRGAGLRVGTDRLVEFARAIEEMDP
                     SRRDDVYWSGRITLTSRPEDIEVYDRAFELFWEKGGAAKARPRQKVRISTPQPDRSVM
                     PPKKTVERNESGTEAVRLRYSPVEVLRRKDFALYSPEEFAALQRLLADLRLSGALRRT
                     RRLEPATRGRHDPRRTLRGAMRTGGEAVRHRFRKARVQPRRVVLLCDVSGSMAPYSRA
                     LLRFLHAGVVAGGHLEAFSIGTRLTRITRELATHDPDRALRQAAGAVQDISGGTRLGD
                     AIKEFVDRWGQRGMARGAVVVIFSDGWDRGDVSVLAEQMQRLSRLAYRIVWVNPLKA"
BASE COUNT         2253 a         3736 c         3924 g         2021 t
ORIGIN      
        1 ggccgccacg tccgcactct aattccgcgc agcttaagca tagtctaatc cggccaggaa
       61 cttggcgcag gggcccgtca cgccctcgtg agaggttggc gtgatccccg aatattgcga
      121 gcagggcgcc ggggttgcgt gtgttgctcg acgcggactg tggctgccgt cccgactcat
      181 tcctggggcg cgcagcgggc ccaggcgctc tgccagaacg attggagtag ctgtggtgga
      241 tgccatcgac gaactcggaa ggggccgtga gtcctacgca aggcgggcgt ggatggacgc
      301 ctacaagtcg ctctcacgag cggatcaaac gactccgctg ggaggggagg aactcgggct
      361 gctcgcgacg tcggccttca tggtcggtcg cgacgacgac ttcctgggct acctggagcg
      421 agcccatcac gcgtacatgg acgccggcga gacgatgcgc gcagtgcgct gcgcgttctg
      481 gatgggcatc aatttcgcgc tccggggaga gatgagtcgt gcgaccggat ggctgggccg
      541 cgcccatcgg ctcgtcgaac gcgaggaggg tgactgcgtc gagcggggct atctgctcgt
      601 gccggttatg ttgcggcatg aagccaacgg taattgggac gccgcgtaca ccgccgcggc
      661 cgaagctgca gagatagccg agcgctttag tgatgcggac ctgctcgccc tcgccttaca
      721 cgagcagggc ctcattctgg tcaaccaggg tcgggtcgag gaaggtctcg gactggtgga
      781 cgaggccatg gtcgcggtgg cctcgggtga gctgtcgccc atcgtcaccg gtatcgtgta
      841 ttgcagtgtg atctctggct gtcaggaagt gtatgcgctg cgccgcgccc aggagtggac
      901 cgccgccctg acacagtggt gcgaggagca gcccgagatg gtggccttct caggccaatg
      961 cctggtgcat cgcgccgaga tcatgcaact gcacggtgcg tggctggacg cgctggacga
     1021 ggcgcttcgg gctcgcaagc gttgcgagca ggggaagaac cggatggcta ccgcccaggc
     1081 cttctaccgg cagggggagg tccatcgttt gcaggggaat tacgccgcgg ccggcgacgc
     1141 gtaccgggac gccagccgat acgggtggga gccgcagcct ggactcgccc tgatgtggtt
     1201 tgccgaaggg aaaggcgacg ccgcgtgcgc cgcgctccgc cgggctctgg gcgagaccac
     1261 caagcccctg aagcgtgcga ggctgctgcc cgcctacatc gagatcatgc ttgccgttgg
     1321 cgatgtcgag gaggcgcgca gcgcctgccg cgagttcggg gagatctcgg ctcgctacga
     1381 gagcggcatg ctggatgcga tggttgcgca cgccaaggga gcggtagatc tgaccgaagg
     1441 cgacgcacgg gactctctgc tcgcgctgcg tcgcgcgttg cagttgtggc aggaactcga
     1501 ggtgccgtac gaggccgcac gcgcgcgtgt gctcctgggt cttgcgtgca gcgcccttgg
     1561 tgaccacgac accgccgagt tggaactgga agccgcccgg agcagcttct cagacctggg
     1621 agcggcgccg gacctcgccc gcgtcgactc gctcagccgg cgcgctccgt ccggcgatat
     1681 tcatgggctg acgccgcgtg agctgcaggt gctgcgcctg gtggccgccg gcgagaccaa
     1741 caaggccatc gccgccgggc ttgtcctcag cgagaggacg gtggacaggc acgtgagcaa
     1801 cattttcgcc aaactgggcg tgtcctcgcg cactgcggcc acggcctacg catacaagca
     1861 ccagctcgtc taagcacact cctcatgggt ggaattaccc acgccgccac atggcggaag
     1921 ttgggtggtt accccgatgc gccacctcct cgtcggctcg tatcttgtga tcgcacagag
     1981 gcgagcgagg agcaacatga ctttcacgtt agacgcactt agtctccagg ttcttcctcc
     2041 ggagatggct ttgcgggagg ccgttcccat tctgtaccgc ctggttgggg atcccacctt
     2101 tctggattcg cacgtacttg ctcatctgga ggaagcggga agggccgagg attggtacgt
     2161 cgcctttcgc gacgaccgct cctgttcgtt gcaggtcttc gtctggcctc cgggaagccg
     2221 gactcagatc cacgaccatt ccacctgggg agccttctgt tgcgtcgtgg gcaccgtcct
     2281 ggaagaacgc ttcgagcgtg ccgacgactg ctccctgacc gacttcgccc gcttgaagaa
     2341 gctctggcgg ctcgagtgga gaaggaaaga tgggatctcg accgtgctgc cttatgagag
     2401 aggcattcac agggtgggta acccgacggg agaaccagcc atctcggtac acctctacgg
     2461 tcccaggctc ggggagatag acggaagaga ctacgaccct tcccaaaact acgtctgcga
     2521 cagaccggaa gaccgatgag atgcgaggca cacaaactga caggggagtc caggttgtat
     2581 cacaagcagg tatcggccag gggcgacatg agaagcggtg tgggaatccc tgccctatcc
     2641 gggcctggcg aggaccttgg ttacctgacc catcaccgga ttttcgcgtg cctgccgagg
     2701 gctgcacgtt acaggaaatc acaccggaaa aatccagcaa ggaggagaag atgagcaagc
     2761 ataggtacgt gaaggaccat gaccggcatc tagctgacag cacggagtac gtcgagacgg
     2821 tgatcgtagg cggcggtcag gctggcctct cggtcggata ccatttagcg agacgagatc
     2881 ggccgttcgt gatactggat gcgaacgagc gcgtcggcga cgcctggcgc aagcgctggg
     2941 actcgcttcg cctgttcacc cctgcccgat acgacggttt ggagggcctg cccttccccg
     3001 cgcccgccgt gaccttccca acgaaggacg agatggccga ctacctcgag tcctacgcag
     3061 cgcacttcga tctgcctgtt cgaaccggcg tcaaggtgga cggactctcc aggcaagatg
     3121 gccgctacgc cttgacatcc ggcgatcggc gtttcgaagc ggaacacgtg gtcgtcgcgg
     3181 ccggcgctca tcaaattccg aagatacccg ccttcgccaa cgagctcgat tcgagcatct
     3241 tgcagctgca ttcgagccag tatcgtcacc cctctcagct ccatgaaggt accgttctgg
     3301 tggtgggcgc gggcaactct ggggcagaga ttgctttcga ggtgtccagc acgcacccga
     3361 catatttgtc gggcaagcca tcggggcagc tccctgtacg tcacgggccc gctatggcgc
     3421 ggttcgtctt ccccgtggtg cggtttgtcg gccaccacgt gctgaccttg aggacgccca
     3481 taggccggaa gatccagccg aagttcatct cccacagcgc gccgctaatc cgcatcaagc
     3541 tgaaggacct cgctgtcgcc ggggtcgagc aggtgccgag aaccgtgggc atcgagggcg
     3601 gccgccctgc gctcgaggaa ggccgcgtcc tggacgtgtc gaacgtgctc tggtgcacgg
     3661 ggtttcggga agtgttcccc tggatcgacc ttcccatctt cggtgaggac ggacgtcctt
     3721 tgcacgagcg cggcgtcgtg gttggtgagc ctggcctgta cttcgtagga ctggttttcc
     3781 agtacgcagt ttcatccgat gtactacctg gcgtcgggag ggacgcggag tacatcgcga
     3841 agtacatcgc ctcgcgcgaa ccgaagggcc ggtcgaggtc gcacgcgctg gccagagcat
     3901 gaggcgaggc aggagtagcc ggactgtgag gaggagatga tgatccacag ttacacgatg
     3961 accgattcgg tggagggaac gcggcgtacg agtaaaagag aaagcatgtt gaggagcgtg
     4021 agtcaaaaga tcctatgggt ggggaggacc acgatttttt tagttggcct cgccgtgatg
     4081 ctggctgtgg ttctggggtt ggcctctgcc gccctggctg gaaccggggt gggcgatgtc
     4141 ttcaacctcg gcaagaagaa caccgttggc caactgagcc agctggttgg caggtcagac
     4201 agcgccatgc tcaaagtgga aaacaatagc gctggtggtg atgctacggc actcaatttg
     4261 caggttgagc ctggcaacgc ccctatgaaa gtcaactctt ctaccagggt gtccaacctg
     4321 aactccgacc agctggacgg caaggatgcg agtaccttca tgccggccag tatctacacg
     4381 gtggatgaaa cagtcgagat gggttctaac gtgaccggca cggttcgcgt ggtcttctgc
     4441 gatgagggag acgtgccgat aagcggtggc ttcagcgggc tggggtccca gacgcagatc
     4501 aaacaatcct ctcgtgtatt ttacgttggg cgtcctacgt ggtgggtagc ggcgaacaag
     4561 ccggtcggtc ccgaggacac atggacagca caggcagtct gtgcagactt cggggagatg
     4621 cataagtaag gagtgactac gtgaaacctc gcagggctgc ctctcgtttc gcccgacaag
     4681 ggtttaggta gccccaccag gtggggttcc cgagaaccac ctactgctac cggcctttgg
     4741 gttaacatac cccgttggaa cgaaggaacg agggtggatg gaagaatcga aagagaagac
     4801 tgtggagagg gagagccccg aggttcggaa ccgctatccc cacttcctga ccgtaccgac
     4861 gcgctggatg gacaacgacg tctacggaca cgtcaacaac gtcgtctact actcgtactt
     4921 cgacacggta gtgaacgagt acttgatctc cacgggcgtc ctcgacatcg aaacgagccc
     4981 cgtcatcggg ctcgtcgtcg agacgcagtg ccggttcttc aagccgatca ccttccccga
     5041 tgtcgtccac gctggcctgc gggtggcgcg tctgggacgc agtagcgtgc ggtacgagat
     5101 cggactgttc aggaatgaag acgacggcgc ggcggcccag ggacatttcg tccacgtcta
     5161 cgtcgagcgc gaatctcgca aagcgaccga actgccgccc gggatgcgcg cggcgctcga
     5221 aaagatcctc gttagctgct agctcgattc gtacgaggcg gccagcgagg cgacgcggcc
     5281 cgagatcagg gccgccgtct cttccagcat ggcttcgtcc acttgcgtga aggccccgac
     5341 ggtatccgag tcgatatcga actcgccgac caccttgccc gcgtcccgga tcaggactac
     5401 gagctcggag cgcgtgtgga tggaacatgc caggtagttc tcgacctcgc gcacgtcttc
     5461 gacgatctgg ttcctgtctt cggagaccgc ggtgccgcag actccctccc cgatgccgat
     5521 gagcgagtga tccgccggcg cggtacgagg acccaagacc agagtatctc ccacgagcag
     5581 atagatgccg ctccagtccc aggacggatg cgcctcgtgc acccttcgca cgacctcgtt
     5641 caagacctca tcgtcgggcg catcggaggc tacgagatcc tccacctctg tcttcaagtc
     5701 ctcgcgaagc cctgtcttct cactcatcga tccctccaca tagtacgacg tctcccacca
     5761 atgataacgc cgccacacgg caaaatccgt gaatcgtctc ccgaaagaaa ccccacaggg
     5821 acgacgacct cgctcgtcgg gtcttggggc acgctgatcg acttccggca tgtgggagat
     5881 agctcgggac aaagtaccct cgaaatgcac gataaaaggc gtcgtgctag actgcccgca
     5941 cttccgatga ggaaggggaa tggggagcaa aatcgccgga tacagtgggg atcgcggcgc
     6001 atgtaatctc gttgacggcg gtctggggaa ggccggagga acgtggattt caggcaggtt
     6061 atacggggtc tgtttcggga aaggagagag ggttgcgtag agtccagatg acggtagacg
     6121 ggcgcgccca cgtggcggag gtagagccga ggttgctcct gctccaccac ctcagggaga
     6181 atctggggat aggcggcgtg cattcgggat gcaacacgac caactgcggg gcgtgcacgg
     6241 tccacatcga cggggagtcc gtcaagtcgt gtacggtcct aaccgtgcag gctgacgggc
     6301 acgagatcac caccgtcagg ggcatcatgg acaacgggct ctggcacccg atgcagcagg
     6361 ctttccacga ggcgcacggg ctccagtgcg gctactgcac gccgggtatg atcatggcct
     6421 caatcagcct cctgaaggag aaccctgacg cgacggagca ggagatcaga gacggactgg
     6481 aagggaacct ctgccgctgt accggctacg agaacatcgt gaaggccgtc cagctcgtcc
     6541 aacagcacac aaacggcgcg gcgtcctaga ggaggaagtc aagtgaccga agcaccggag
     6601 aagttcgtcg gaggcggcgt accgcgcaag gaagaccctg ccctggtaac tgggcgggcc
     6661 aactggacag acaacatcaa gctgcctggg atgttgcacg cgacgctaat gcgcagcccc
     6721 ttcgcccacg cgaagatcac atccatagac gtatcggccg cgagggagca gcctggcgtc
     6781 gtcgctgtct ttacgggcga cgacctcgct gacgattggg acaagaaccc ccaggcgaat
     6841 cccgcatccg tcacaggcta cggcattagc ctggcggagc gggcccaggg cgcctcctcc
     6901 gccacggaca ccgccgacac cacagacggg cagcccgcgg acgaagagcc gaagaccttc
     6961 gtctcctgga ttcctacgga cgacacgaag atccccaatc actggcccgt cgcccgcgac
     7021 gaggtcaact acgcgggaga catagtggcc gtcgtcgtgg cgaccgaccg ctacaaggcg
     7081 caggacgccc tcgagttcat cgaggtcgat tacgacccga tgacgcctgt actcgacgtc
     7141 gaagaggccg tggctgaagg ctccccgctc gtgcacgaag acataggcac gaacgagagc
     7201 tttacctgga cgctcgggac aggggacata gatgacgctt tcaacagggc cgacgtagta
     7261 gtcaaggagc gctacctgca gcagcgtctg atccctaacg ccatcgagac gcgcggcgtc
     7321 gtcgcgaacc ccgatccggt aaccggcggc tacacgctct acagctcgac ccagatccca
     7381 cacatcctga agtattgtct cgccgtgtac tgcggcgtcc cagagcagca gctccgggtg
     7441 gtcgcgcccg acgtcggcgg gggattcggc tccaagctca acgtttacgc cgaagaggcc
     7501 atagcgctgg tactggcgcg caagctcggc gtgcccgtca agtggatcga ggacaggtcc
     7561 gagaactacc tggcgacgat ccacggtcgc gggcaggtac aggacatcga gctcgccgcc
     7621 acttctgagg gcaagatcct cgggatgcgg gtgaaccttc tcgccgacat gggcgcctac
     7681 ctgatgctcg acgggccagg catccctatc ctcggcgctt tcgtctatcc gggcgtctac
     7741 actttcggcg cctacgactt cagcgtcacc ggcgtcttca ccaacatgac cccgacggac
     7801 gcctaccgcg gcgctggccg tcccgaggcg atcttcgcca tagagcgcat catcgaggct
     7861 cttgcgcgtg aggtcgggct ggaccctctg gaggtccggc ggcgcaactt ctacgagcct
     7921 ttcgacgagc cgacagatac ccctgcgggt atccagtacg actcgatgaa tatgcagccg
     7981 gcgctggacc gcgtcctgga gctcgccgac tacgaggggt tgcgcgagga acagcggcgg
     8041 cggcgtgaga acaacgaccc cgtccagctc ggcatagggt tctcgaccta tacggagatc
     8101 tgcggcctcg ccccctcgca ggtgctcgcc ggcctgcgca ccggcggctc gggatgggag
     8161 acggccaagg tccagatgct cgcctcgggc aaggtcgagg tcgtcacggg cacctcgccc
     8221 cacggccagg gccacgtcac gagctggtcg cagatcgccg ccgacgccct cggcgtcgac
     8281 gttaacgacg tcgagatcct gcacggcgac accgcgctgt caccgtacgg acgtgacacc
     8341 tacggctcga ggagcctgcc cgtcggcggg gtcgcggtac acctggctgc ccaggacgtg
     8401 gtcaacaagg caaggaagat agcggcgcac atgctcgaag cggccgaagg cgacatggag
     8461 ttcgaaggcg gcaagttcag cgtcgcaggc tctccggatc agagcgtcac catacaggag
     8521 gtcgcgacgg cggcctacat gaactacgac ctgccagcgg gtatggagcc tgtcctgtct
     8581 gaagaggtat tctgggaccc gcccaacttc acgttcccgt tcggggcgca cgtctgcgtc
     8641 acggaggttg atacggagac tgggttcgtc aagataaggg actacttcgg cgtggacgac
     8701 tgcgggcccg tcatcaaccc gacaatagtg gatggacagc tccacggcgg catcgcccag
     8761 ggcgtggcgc aggccctcta cgaggaggcc gtctacgacg aggaggggaa cctcacgacg
     8821 ggctcgatgg tggactacct gatccccggc gcccccgagc ttccgaacta cacactggag
     8881 cgcaccgtga cgccgtcgcc cacgaacgtg atgggagtga agggcgtcgg cgaggcaggg
     8941 accatcggat cgcctcccgc ggtcataaac tctgtcgtgg acgctctctc gcccttcggc
     9001 ataacgcaca tagacatgcc ggcctcgccg tggaaggtgt ggcaggcgat ccaggacgca
     9061 caggggcagg atgcgggcac gcgcgacgca gacctctcgg gccgcccgtc ccaggcgcct
     9121 gacagcacgg ataccgaagg agggagcacg tcatgatccc catcgccttc gactacgagg
     9181 tcgccgaatc cgtcgaccac gccatcgagc tgctgggcca acacggcgac gaagccaagc
     9241 tcctcgccgg cggccacagc ctgctcccgc tgatgaagct caggctcgcc gccccgacgg
     9301 tcctcgtcga tctcggacgc ataggcgacc ttaactatgt acgagatgag ggggatcacc
     9361 tcgccatcgg cgcgatgacc cgccactgcg acatggaacg cgacgctact ctcaaggagc
     9421 attgcggtct gatctcctac acggcctcgc tcgtcggcga cccgcaggtg aggcaccgcg
     9481 gtacgatcgg cggctccgtg agccatggcg acgcagcttc ggacctgccc tcggctctgt
     9541 tggctctcga cgccatcttc gtcatcaagg gccccggagg agagcggacc gtggccgcgg
     9601 gagacttctt cgaggactac ctgcagacca ccctggcccc cgacgaggtg ctcactgaga
     9661 tacgggtctc caaactgggc ggcgcgggct ggtcgtacaa gaagttcaac cgcagggccc
     9721 aggactgggc cgtcgtcggc gttgccgccg tagtcgagcg ctcgaacggc accataggct
     9781 ctgcccgcat cgggctgacc aatatgggct cgacgccagt gagggcgacg gccgccgaga
     9841 acgctctcgc cggcgccgac cccgcctcgg tagccgaggc gacaggctcg gccgacgagg
     9901 gcacgagccc ttcctcagac atcgcggcct cggccgagta ccgtcgccac ctggcacgcg
     9961 tcctcagcag gcgggccgtc gaggaggcat tgggacgttg agcgaagtgt ctagcgaacg
    10021 ccggggggcc ctgcctcccg gcgtttctca tttccgggag ctgggcgagc agcttcggga
    10081 gcacgactat ctttctgacg aaggtctatc caccgcgatc ttcctctccc tcaagctcga
    10141 gaagccgctc ttcctcgaag gcgaggcagg ggtcggcaag acggaggtag ccaaggtact
    10201 ctcccgcatt ctggatactc ctttgatccg acttcagtgc tacgagggca tcgacgtcta
    10261 ccaggccgtc tacgagtggg actacgcccg ccaactgctg cacatccgtg cagccgaagc
    10321 ctttggcggc gaggaccggg agcgcgtgga gcgcgacctc tacgaccgcg agttcctggt
    10381 gagcaggccg ctcctgcagg cgctggagca tcgcggtccc atgccgcccg tcttgctcat
    10441 agacgaggtg gaccgctccg acgacgagtt cgaggctttc ttgctggaga tcctctctga
    10501 tttcgccgtc accatccccg agattggcac catcagggca gaacgcccgc ccgtcgccgt
    10561 actgacctcg aaccgcaccc gcgaggtgca cgacgcgctc aagcgccgct gcctctacca
    10621 ctacatcgag caccccagct tcgagcggga ggtcgagatc ctacgcctca gggtgcctga
    10681 cgcggaggag aggttggcac gcgaggtcgc ggcggcgacg gagaagatgc gcgccatgga
    10741 cctctacaaa ccacctggcg tcgccgagac actcgactgg accgaggcgc tcgtcgccct
    10801 cggcgcgagg gagctcgacg agcagctcgt ggaggccact ctaggctccg tcctcaaata
    10861 tcgtgaggac cagcaaaggg ccgccaacgc cgggctcgcg gcgctcatcg gaggggtgag
    10921 agtagtaggg aacgatgcct gaagggagca caccgccgat gctcgacgct gcggttgcgt
    10981 tcgggcgcgt gctgaggggg gccggattgc gggtcggtac ggaccggctc gtcgagttcg
    11041 cccgcgccat agaggagatg gacccatcgc gccgcgacga cgtgtactgg tcgggaagga
    11101 tcaccctgac ctcccgaccc gaggacatag aggtctacga ccgcgccttc gagcttttct
    11161 gggagaaggg aggcgccgcg aaggcgaggc cgcgccagaa ggtgcgcatc tccacgccgc
    11221 agcccgaccg ctccgtcatg ccgccgaaga agaccgtcga gagaaacgag tcgggcacgg
    11281 aggcagtcag gctccgctac agccccgtcg aggtgctgcg tcgcaaagac ttcgccctct
    11341 actcgcccga ggagttcgcc gcgctccagc gccttctcgc cgacctcagg ctctcggggg
    11401 cgctcaggcg cacccggcgg ctggagcccg ccacgcgcgg acgccacgac cctaggcgca
    11461 cgctgcgagg cgcgatgcgc accggcgggg aggccgtgcg gcatcgcttc cgcaaagcgc
    11521 gggtgcagcc gaggcgcgtc gttttgctct gcgacgtctc cggttcgatg gccccgtaca
    11581 gcagggcgct cctgcgcttc ttgcacgccg gcgtcgtcgc agggggacat ctcgaagcgt
    11641 tctccatagg aacgcgcctg accaggatca cacgcgaact cgcgacccac gacccggaca
    11701 gggcgctcag gcaggcggcc ggcgcggtac aggacatctc cggcgggacg cggctggggg
    11761 atgcgatcaa ggagttcgtt gaccgctggg ggcagcgtgg catggcgcgc ggtgccgtcg
    11821 tcgtgatctt ctccgacggc tgggacaggg gcgatgtctc tgtgctcgcc gagcagatgc
    11881 agcgcctgag ccgcctcgcg tacaggatcg tctgggtaaa tcctctcaag gccg
//
LOCUS       DATAIR010000253         5033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_146115, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000253 DATAIR010000000
VERSION     DATAIR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146115"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHM69_06075"
     CDS             complement(<1..227)
                     /locus_tag="VHM69_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739994.1"
                     /translation="MSDRSLERMGAACGILYVVLIYVGSSIGSLESSTARTVLVFLGL
                     LVFMFFLGSLWSAMRRAEGGSGWLSTIVFGAG"
     gene            complement(276..1211)
                     /locus_tag="VHM69_06080"
     CDS             complement(276..1211)
                     /locus_tag="VHM69_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2739995.1"
                     /translation="MAVPAATQQPAPHSALRRLVARHPVAAFLVMVYTVSPAVALVPF
                     LTRRDILPFDLALYDSFAPIFGVALPAFIVVAATGGRDGVQDLARRCLRWRVGVRWYL
                     FAFFSVPVAVMLCASIVFGRAQLDMLVDKWELLFTAVLPQLLFLILFFIVAEEVGFMG
                     FLQARLQDRYGPLKASVIVTLPFALYHLPTLMVENGFGLAQLHIALGFLGILAVLQMF
                     GRIVIMWLYNVTGYSVLLVGLFHSSFDATTSAFGRTFVVPGPAGTAFLSGFVIPSAVV
                     ALLAVMVVVFTRGRLSYKPDRLSQSTTLARPRAHG"
     gene            complement(1266..1844)
                     /locus_tag="VHM69_06085"
     CDS             complement(1266..1844)
                     /locus_tag="VHM69_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2739996.1"
                     /translation="MVNRIRTTGILLATGGVFFILSFGPEAIGLVRPERDGQIVNFPL
                     YAFFTGFMVVATVLLGVGLWRMRAWTSGIGRIGRVGLYFCIASVAGIGLTAVTLFVSA
                     VQAAQPPEYTFVFFALGLLFSIIGPVLLGTGLRHVGWLGPGRMLPFVVAGGTILAWVP
                     TDPWHDIGLLVLGLSWAGLGTSMLLRRPDEGK"
     gene            2409..>5033
                     /locus_tag="VHM69_06090"
     CDS             2409..>5033
                     /locus_tag="VHM69_06090"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2739997.1"
                     /translation="MANDPFGARATLNTSTGDVGFYSLKKLDENVSGDVFSLPFSIRV
                     MIESLLRNAGGKFVSKEDVEALADWPESIGGELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMRNVGGDPGRINPLVPTDLVIDHSVQVDAFGSPAALQFNAEREFERNRERYEFLKW
                     GQQAFDNFSVVPPATGIIHQVNLEYLAKGVIVKDGVAMPDTLVGTDSHTTMINGLGVL
                     GWGVGGIEAEAVTLGQPYYMLVPEVIGFKLTGALQEGVTATDLVLTVTQMLRSHGVVG
                     KFVEFYGEGLSKLSLPDRATIANMSPEFGATCTFFPVDTETMRYLRGTGREEELVELV
                     EAYSKEQGLWRTDETPEPRFSEVLDLDLTSVEASLAGPRRPQDRVALDDMQPSFRQAL
                     AEMVDPDHPLLGNGHADAYDEADEESFPASDPPNPDIHIADDTESFPASDTPSGSADV
                     TNEGGGDPTSGGEPEGTGPEAEPDGSVTVTVDGDELHLKHGSAVIAAITSCTNTSNPS
                     VMMGAGLLAKKAVEKGLSVAPHVKTSLAPGSKVVTEYLQTSGLLDPLERLRFDVVGYG
                     CTTCIGNSGPLAEEISAAVEENDLVVAAVLSGNRNFEGRINPDVRANYLASPPLVVAY
                     ALAGTVDIDLSRDAIGEDRDGSPVYLKDIWPSQEEVAREIENALDPEIYREQYADVYT
                     GNEQWNDVEVPSGDLYEWDPASTYIQEPSFFKDMGPEAEDLKDIEGARVLVKVGDSVT
                     TDHISPAGAIPSKMPAGKYLIEKGVDPRDFNSNGSRRGNHEVMVRGTFGNIRLRNQLV
                     EKEGGYTIHLPDGEETTIYEASLKYEEENVPLVVLAGKEYGSGSSRDWAAKGSFLLGV
                     KAVIAESFERIHRSNLIGM"
BASE COUNT         1081 a         1581 c         1536 g          835 t
ORIGIN      
        1 cctgcaccaa agacgatggt cgaaagccat ccgctgcctc cctcggctcg gcgcatcgcg
       61 ctccacaggc tcccgaggaa gaacataaag acgagcaaac cgaggaacac caggacggtc
      121 ctggccgtgg acgactcgag gctgccgatg ctgctcccga catagatcag caccacgtac
      181 aagatgccgc acgcagcccc catcctttct aacgaccggt cagacatgac gatctctcct
      241 ttccttccga gggttttctt gctgcccgaa gagcatcagc cgtgagcccg aggccgagcc
      301 aaagtcgtcg attgggaaag gcgatccggt ttgtacgaga gccgtcccct ggtgaacacg
      361 actaccatca ccgcgagcaa ggcgaccacg gcgctgggga tcacgaatcc cgataggaac
      421 gcagtccctg caggtcctgg gacgacgaat gtgcggccaa acgccgaagt cgtcgcgtcg
      481 aagctggagt ggaagagtcc caccagaagt acgctgtatc cggttacgtt gtagagccac
      541 atgatgacga tgcgtccgaa catctggagt accgccagga tgccaaggaa cccgagcgcg
      601 atgtggagct gcgcgagccc gaatccgttc tcgaccatca gggtgggcag atggtagagc
      661 gcgaatggga gtgtgacgat gacgctggcc ttgagaggcc cgtacctgtc ctgtagcctc
      721 gcctgcaaga accccataaa gccgacctcc tcggcgacga tgaagaacag gatcaggaac
      781 agcagctgcg gcaacaccgc ggtgaacagt agctcccact tatccaccag catgtccagc
      841 tgtgctcgac cgaagactat acttgcgcac agcattaccg ctacgggcac gctgaagaac
      901 gcgaagagat accagcggac gccgacgcgc cacctcagac acctgcgcgc cagatcctga
      961 acaccgtctc tcccaccagt ggccgcaacc acgatgaagg ccggcaacgc caccccgaag
     1021 atcggggcga acgaatcata cagggcgagg tcgaacggca agatgtctct tcgtgtcagg
     1081 aacggcacca gcgcgaccgc ggggctgacg gtatagacca tgaccaggaa ggccgccacc
     1141 ggatggcggg ccaccagacg cctgagcgcc gagtgaggag cgggttgctg ggttgcggcg
     1201 ggtacggcca tgatcggtat cctttctggt caggcgctgg cgcagttcgg tagcatagag
     1261 tccgcctatt tgccttcatc cggtctacga agcagcatcg aggtccccaa ccccgcccag
     1321 gaaagcccaa gtactagcaa gcctatgtca tgccagggat ccgtgggaac ccaggcgagg
     1381 atcgtgcccc cggcaacaac gaaggggagc attcgaccag gcccaagcca acccacatgc
     1441 cgcaatcccg tgccaagcag aacagggccg ataatcgaga aaaggagacc aagggcgaag
     1501 aacacgaaag tgtattcagg aggctgcgcc gcctgaacgg cggagacgaa aagggtaacg
     1561 gccgtcagtc cgatgcccgc aaccgatgcg atgcaaaaat agagtcctac ccggccgatc
     1621 cgaccaattc cagatgtcca ggcacgcatg cgccacaacc cgacacccag caagacagtt
     1681 gccacgacca taaagccggt aaagaaggcg tagaggggga aattgacgat ctggccatca
     1741 cgttctggac gtaccaggcc gatcgcttcg ggaccgaagg aaaggatgaa gaacacccca
     1801 cccgtcgcga gcagaattcc agtagttcta attcgattta ccatctttac ctccgtcagg
     1861 gtcttcgatc tgagaagcga tcacgcatct aagccccttc ccaaacaagg cgcgccgggc
     1921 attcgataac agcccgcaac gaggttcctg tcagcggtga cacgggaacg ggtagtaccg
     1981 gatacggacg tgatctcagt tagaatctta gcctcacctg ctacggaagt cgtttgcatt
     2041 tttgcttcct tcgagcgttt tcctgtagcc ctttgtgaac atggtacgag acggctcttc
     2101 ctaccgtcag aggcagaagt cctgaattat taggaaatgt tcccgaagtt tgggaacggt
     2161 ttcccgaagc gatggcccgg agaagcgtgt ccataccatg aataatcgcc ccaagcgcca
     2221 tggactgtca cctgcgtcgt cacggcctga tgctgctcca cggcgacacg gactggttga
     2281 ggtggtcccc tggcgcgccc tctactgaac gggtgcggca ctctgagagc cggtaaacgc
     2341 ggtccgatca tctataatct tctatgcggg tcacacatag cgaatcttag agaagaggtt
     2401 gcgaatatat ggcgaacgat cccttcggtg cacgcgcgac actgaacacc agcactggtg
     2461 acgttggctt ctatagcctg aagaaacttg atgagaatgt ctcgggagac gtgttctcgc
     2521 tgccgttctc gatccgggtg atgatcgagt cactgctgcg taacgccggc gggaaattcg
     2581 tctcgaagga ggacgtcgag gcgctggccg actggcccga gtctatcggc ggggagcttg
     2641 cctatctgcc tgccagggtg gtcatgcagg actttacggg tgtgcccgcg gtcgttgacc
     2701 ttgcggctat gcgcagcgcg atgcgaaacg tcggggggga ccctggcagg atcaaccctc
     2761 tcgtgcctac ggatttggtc attgaccact cggtacaggt cgatgctttc gggtccccgg
     2821 cggcgctcca gttcaacgcc gagcgcgagt tcgagcgcaa ccgcgagcgc tacgagttct
     2881 tgaagtgggg ccagcaggcc ttcgataact tctccgtcgt cccgccggcc acggggatca
     2941 tccaccaggt caacctggag tacctggcga agggcgttat cgtgaaggac ggagtggcga
     3001 tgccagacac gctcgtcggc accgacagcc acaccaccat gatcaacggc ctcggcgtcc
     3061 tcggctgggg cgtcggaggc atcgaggcgg aagcagttac cctcggccag ccttactata
     3121 tgctcgtccc cgaggtgatc ggcttcaagc tgacgggcgc actgcaggag ggcgtaacgg
     3181 ccacggacct cgtcctcacc gtgacgcaga tgctccgctc gcacggcgtc gtcggcaagt
     3241 tcgtcgagtt ctacggcgag ggactctcta agctctcgct ccccgaccgc gccacgatag
     3301 cgaacatgtc ccccgagttc ggggcgacgt gcacgttctt ccccgtggac accgagacga
     3361 tgcgctacct gcgcggcacc ggccgcgagg aggaactggt cgagctggtc gaagcgtaca
     3421 gcaaagaaca ggggctctgg cgtacagacg agacgcccga gccgcgcttc tcggaggtgc
     3481 ttgatctgga tctgacatcg gtcgaagcga gcctcgcggg gccgcgccgc ccgcaggaca
     3541 gggtcgccct ggacgacatg caaccctcct tccgccaggc gctggcggag atggtcgatc
     3601 cggaccaccc tctcctcggg aacgggcacg ccgatgccta cgacgaggcg gacgaggaat
     3661 ccttccctgc gagcgatccc ccgaacccgg acatccacat cgccgacgac accgagtcgt
     3721 tccccgcgag cgacacgcct tcggggtcgg ccgacgttac caacgagggt gggggagacc
     3781 cgacctcggg tggcgagcca gagggcaccg ggcccgaagc tgaacccgac gggtccgtca
     3841 ccgttaccgt tgacggagac gagcttcatc tcaagcacgg ctcggccgtc atcgcagcca
     3901 ttacgagctg cacgaacacc tcgaaccctt ccgtgatgat gggcgccggt ctgctggcga
     3961 agaaggccgt cgagaaaggc ctctccgtcg cgcctcacgt caagacaagc ctcgcgcctg
     4021 gctccaaggt cgtcacagag tacctgcaga cctcggggct cctcgacccg ctggagcggc
     4081 tcaggttcga cgtcgtcggc tacggttgca cgacgtgcat cgggaactcc ggtccgcttg
     4141 cggaggagat ctccgccgcc gtcgaggaga acgacctcgt cgtcgcggca gtcctctcag
     4201 gcaaccgcaa cttcgagggg cgcatcaacc ctgacgtcag ggcgaactac ctcgcctccc
     4261 cgccgctcgt cgtcgcctac gcgctcgctg gcacggtgga catagacctg tcgagagacg
     4321 ctataggcga ggatagagat ggcagccctg tttacctcaa ggacatctgg ccctcgcagg
     4381 aagaggtcgc cagggagatc gagaacgccc tcgaccctga gatctacagg gagcagtacg
     4441 ccgacgtcta cacgggcaac gagcagtgga acgacgtgga ggtgccctcg ggcgatctct
     4501 acgagtggga ccccgcctcg acctacatcc aggagccctc tttcttcaag gacatgggcc
     4561 ccgaggccga ggacctgaag gacatcgagg gggcgcgcgt gctcgtgaag gtcggcgact
     4621 ccgtgaccac ggaccacatc tcgcccgccg gcgccattcc ctcgaagatg ccggcaggaa
     4681 agtacctcat cgagaagggc gtggacccgc gcgacttcaa ctccaacggc tcccgccgcg
     4741 gcaatcacga ggtcatggtg cgcgggacat tcggcaacat caggctccgt aaccagctcg
     4801 tggagaagga gggcggctac accatccacc ttccagacgg tgaggagacc acgatctacg
     4861 aggcgagcct gaagtacgaa gaggagaacg tgccgctcgt cgtcctcgct ggcaaggagt
     4921 acggctcagg ctcctcgcgt gactgggccg cgaagggctc attcctcctc ggggtcaagg
     4981 ccgtcatcgc cgagagcttc gagcgcatcc atcgctcgaa cctgatcggc atg
//
LOCUS       DATAIR010000254         7016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_293313, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000254 DATAIR010000000
VERSION     DATAIR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293313"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..975)
                     /locus_tag="VHM69_06095"
     CDS             complement(136..975)
                     /locus_tag="VHM69_06095"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2739998.1"
                     /translation="MNLPIRSYLYVPGYDARKIEKALASESDAVVLDLEDAVAPNRKE
                     EARETVVQVLKSEPEKPVFVRVNAVGTELAAADIDAVVGRHLAGLRLPKIEYVESVRL
                     VAETLKELRSEAGIQCLIESALGLELAFEIANSHVRVTGISLGEADLAADLGVSGEAG
                     LLYARSRIVTAARAAGLPHPVQSVYTNVRDLDGLRRSTEEGKRLGFVGRSTIHPSQVS
                     VVNEVFTPTEEEVTEAQELVDRLDSGSTSGAFLLGDGRFVDRAIVESARLTLDLARRS
                     GAR"
     gene            complement(972..2162)
                     /locus_tag="VHM69_06100"
     CDS             complement(972..2162)
                     /locus_tag="VHM69_06100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2739999.1"
                     /translation="MTEQKALAGIKVIDAATLFAGPLAATLLGDFGAEVIKVEHPRGD
                     PSRSHGYSKDGVGLWWKMLGRNKKCVTLNLSKPEGQEIFERLVRDADVVIESFRPGTL
                     ERWNLGYERLAEINPGLILARVTGFGQFGPYKNRPGFGTLAESMSGFAHVTGLPDGPP
                     TLPPFGLADGIAALATAVSILTALRARDTNGRGQVVDLAIIEPIFTVLGPQPTVYDKL
                     GVIQQRSGNRSVNNAPRNTYETSDGHWVAVSTSAQNIAERVMRLVGHPEYIEEPWFQK
                     GSERAKHADELDEVVGAWISARTQEEVVAAFEEAGAAVAPIYDIEGIMQDPQYEALNS
                     IITVDDPELGPTKMQNVLFRLSETPGEVRWSGPTLGEHNEEVYREIGIDKEELGKLAE
                     KGIL"
     gene            2298..3758
                     /locus_tag="VHM69_06105"
     CDS             2298..3758
                     /locus_tag="VHM69_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX2740000.1"
                     /translation="MGRFDTVVLGGRAVLPGRGLVRADVGIKDGRVAAVGEDLSPNDA
                     DESINAEGKLVLPGAVDAHFHLGIYRDITEDTKSETLSSLAGGVTSIISYFRTGSHYL
                     EKSGPYAEIFPEVLEATNGNSRVDFGYHLAPMVRDQVGEIGRLVDSEGVTSFKYYMFY
                     KGLDLSGASSDASGYTMSENYDLGHLFEIMEQVAKAQEERTDGSRVSLSIHCEQPELI
                     RVFSERVKEEGVLSGLEAYSASRPTLTEHLAVAEVAVLAGHTQCPINLLHLSSEEAFE
                     AALELKALNPYLDVRLETTLHHLALTYENYSDQRGKVNPPIRAQSDVDALWRGVVRGE
                     IDWICSDHACCSEEHKEGDLWQALPGFGGTPLIYPFILTEGPRHGLSLERCVELVATN
                     PARAYGLSPRKGAIIVGADADLAIVDMDEIHPVTTERLLSAQDYTPFEGMELTGWPVR
                     TILRGRTVFADGEPVGEPSGTYLQRPLTTEQPLAAP"
     gene            3780..5144
                     /locus_tag="VHM69_06110"
     CDS             3780..5144
                     /locus_tag="VHM69_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2740001.1"
                     /translation="MNYASLVVVGAGACGMVAALAAAKRGVRVLVLEKGNEPAGNTAR
                     STGLIPAAGTRFQREVGILDDSPELMAEDIFRKNGYESDPGITKLLCEQSGPLVEWLV
                     DEAGCEMVCYTDFLYPGQSLHRMHGPKDGYGSELVQQLENAIRDDPRIELFTNAAVDG
                     LLSEDGMVTGVETSDGEILADAVVLALNGFGGDREMVERYLGPEAAAALYFGSPNNTG
                     EGIRWGMALGAATGHMGSYQGHASVAFPDGPLVSWGVVVNGAILVNREGRRFGFEIVG
                     YSEYAGAVMAQPGGEAWEIFDAEVYSASLGTRFDEVVEAGKVVTSETVEDLAETLGLP
                     AGALAETIEETNRYARGEAQDPFGRDDILGGPLESPFYGIHVRGALFHTQGGLRVDAS
                     ARVLRPDGTHIQSLYAGGGTAVGVSGSGYTGYSSGNGLLAAAVLGKVAGEAAADELSQ
                     RPTK"
     gene            5315..6100
                     /locus_tag="VHM69_06115"
     CDS             5315..6100
                     /locus_tag="VHM69_06115"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX2740002.1"
                     /translation="MGEASVVSGRSSVNVDLTGRSALVTGAASGIGRACAERFSRAGA
                     AVTVLDLDGDAAGRVAENIGGEALQADLADYEVLDDLEVDADIVVNNAGLQYVAPVEE
                     FPPERFSMILRVMLEAPFRIVRKALPGMYERGWGRVINISSIHGMVASPYKSAYISAK
                     HALEGFSKTVALEGGGKGVTSNCICPAYVRTPLVENQISDQARRHGIPEDEVIEKIML
                     TEPAIKRLIEPEEVAEFAAFLCVPEASFINGASLVMDGGATAH"
     gene            6132..>7016
                     /locus_tag="VHM69_06120"
     CDS             6132..>7016
                     /locus_tag="VHM69_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2740003.1"
                     /translation="MTETAEGTLLWQPSEEFKENARISDYMTWLKEEKDLSFGDYNEL
                     WEWSVTDLEGFWGSVWEYCGVEASKPYERVLAKREMPGAEWFPGTELNYAERVLKHAA
                     GRLEEPAILHQSEVRDLGEMTWGELQEKTAALAAALKEMGVQRGDRVAAYIPNIPEAV
                     IALLACSSIGAVWSSCSPDFGAGSVVDRFKQIEPKVLIAVDGYRYGGKDYDRTQVIAR
                     LQEEIPTLQKTVVLPYLTEEPDTSSLQNAVMWDELLSRHEGADLAFEQVPFDHPLWIL
                     YSSGTTGLPKAIVHSQGGI"
BASE COUNT         1268 a         2182 c         2312 g         1254 t
ORIGIN      
        1 ccgtgtcgcc gtggcggcgc atgagcgcca tcctgaacgg aggatggttg ggcgagtggg
       61 gcgtctcggc ggcgagcggc tgcgccaggt cgaagacctc gacgccggcc tcgatcgccc
      121 gtagcagacc gtcgctcatc gtgcaccact tcggcgggcg aggtcgagtg tgaggcgcgc
      181 ggactcgaca atcgcccggt ccacgaaccg gccgtccccg agcaagaacg cgccggaggt
      241 ggacccgcta tcgagccggt cgaccaactc ttgcgcttcg gtcacctcct cctccgtcgg
      301 tgtgaagacc tcgttgacga cggagacctg ggaaggatgg atggtcgagc gcccgacgaa
      361 gccgaggcgt ttcccttcct cggtggaacg gcgcagtccg tcgaggtcgc ggacgttcgt
      421 gtatacgctc tggaccggat gcgggagccc ggcggcgcgc gccgcggtca cgatgcgcga
      481 gcgagcgtag agcagccctg cctcgccgct gacgccgagg tcggccgcga ggtcagcctc
      541 gccgaggctg atgcctgtga ccctcacgtg ggagttcgcg atctcgaacg cgagctccag
      601 gccgagggcg gattcgatca ggcactggat gccggcctcg ctgcgcagtt ccttgagggt
      661 ctccgccacg agccttacgc tctcgacgta ctctatcttc ggtagccgca acccggcgag
      721 gtggcgtccg acgacggcgt cgatgtcagc cgccgcaagc tcggtcccca ccgcgtttac
      781 ccttacgaag accggcttct caggctcgga ttttagaacc tggacgaccg tctcgcgggc
      841 ctcctctttg cggttcgggg cgacggcgtc ttcgaggtcg aggacgacgg cgtcggactc
      901 gctcgcgagc gccttctcga tctttcgcgc gtcgtagcca ggcacgtaga gatagctccg
      961 tatcggaagg ttcacaggat gcccttctcg gcgagcttgc ccaactcttc tttgtcgatc
     1021 ccgatctccc tgtagacctc ttcgttgtgc tctccaagag ttgggcctga ccatctgacc
     1081 tcgcccggcg tttcggagag cctgaatagg acgttctgca tcttcgtggg gccgagctcg
     1141 gggtcatcga cggtgatgat ggagttcagg gcctcgtatt gggggtcctg catgatgccc
     1201 tcgatgtcgt agatgggtgc gacggccgcc cctgcctcct cgaaggcagc gacgacttct
     1261 tcctgggtgc gggccgagat ccaggctccc accacctcgt cgagctcgtc cgcgtgtttg
     1321 gcacgctccg agcctttctg gaaccagggc tcttctatgt actcggggtg cccgactaga
     1381 cgcatcacgc gctcggcgat gttctgggcg ctggtggaga ccgcgaccca atgaccgtcg
     1441 ctcgtctcgt acgtgtttcg cggggcgttg ttcacagaac ggttgccact cctttgctgg
     1501 atcacaccga gcttgtcgta gaccgtcggc tggggcccga ggacggtgaa gatgggctcg
     1561 atgatcgcca ggtcaacgac ctggccgcga ccgttcgtgt cgcgtgccct gagtgcagtc
     1621 aggatcgaga ccgccgtcgc gagcgccgcg atgccgtcgg cgagcccgaa cggcgggagg
     1681 gtaggcggcc cgtcaggcag gcccgtgacg tgcgcgaagc cgctcatgga ctctgctaaa
     1741 gttccgaaac ctggcctgtt cttgtacggg ccgaactgcc cgaagcccgt tacgcgggcg
     1801 aggatgaggc cggggttgat ctcagcgagt cgctcgtagc cgaggttcca cctctccaga
     1861 gtgcccggcc tgaagctctc gatcacgacg tcggcgtccc tcacgagacg ctcgaagatc
     1921 tcctgtccct cgggtttgct caggttcagg gttacgcact tcttgttgcg cccgagcatc
     1981 ttccaccaga gtcccacgcc gtccttggag tagccgtgcg agcgcgaggg gtcgcccctg
     2041 ggatgctcga cctttatcac ctcggccccg aagtccccga gcagcgtcgc ggcgagcggg
     2101 ccggcgaaca gcgtcgccgc gtctatgacc ttgatgccgg cgagcgcctt ctgttccgtc
     2161 aatctctgat ctccttcgat ctctcccaag aaccagcctc tatctttgcc cacgcggtgt
     2221 cctgccgcaa ggttttgacc gggccgcgcg taccgtgcta tgttggcaaa ggaagtactt
     2281 cgggggaagg ggtggctgtg ggtagattcg atacggtcgt tcttggcgga cgcgcagtcc
     2341 tgccgggcag gggtctggtc agggcagatg tgggcatcaa ggacggacgt gtggccgccg
     2401 tgggagaaga cctctccccg aacgacgccg acgagtccat taatgcggaa ggcaagctcg
     2461 tgctccccgg cgcggtggac gcccatttcc acctcgggat ctaccgcgac atcacggagg
     2521 atacgaagag cgagacgctc tcgtcgctgg ccgggggcgt cacgagcatc atcagctact
     2581 tcaggacggg gagccattac ctcgaaaaga gtggtcccta cgcggagatc ttccccgagg
     2641 tcctcgaagc caccaatggg aactcgcggg tggatttcgg ctatcacctt gcgccgatgg
     2701 tccgtgacca ggtcggggag ataggccgtc tcgtcgatag tgaaggcgtt acctcattca
     2761 agtactacat gttctacaaa ggcctcgacc tctcgggcgc ctccagcgac gccagcggct
     2821 acacgatgag cgagaactac gacctcggcc acctcttcga gatcatggag caggtcgcga
     2881 aagcgcagga agaaaggacc gacggctcgc gtgtctcgct ctccatccac tgcgaacagc
     2941 cggagctgat ccgggtcttc tcagagaggg tgaaggaaga gggcgtactc tccggcctcg
     3001 aagcatacag cgcctcccgc ccgacgctca cagagcacct cgccgtcgcc gaggtcgccg
     3061 tgctcgcagg ccatacgcag tgccccataa acctcctcca cctctccagc gaggaggctt
     3121 tcgaggcggc gctcgagctg aaggcgctga atccctacct cgatgtgcgc ctggagacga
     3181 cgctccacca cctcgcgctc acctacgaga actacagcga ccagcggggc aaggtaaatc
     3241 ctcccataag ggcccaatcg gacgtggacg cgctgtggcg tggagtggtg cggggtgaga
     3301 tagattggat ctgctccgac cacgcctgct gctcggagga gcacaaagaa ggcgacctct
     3361 ggcaggcgct ccccggtttc ggcgggacgc ccctgatcta ccccttcatc ctgacggaag
     3421 gcccgagaca cggcctctct ttagagcgct gcgtggagct cgtcgcgacg aaccctgctc
     3481 gtgcctacgg cctgagcccg cgcaagggtg cgatcatcgt cggcgccgac gccgaccttg
     3541 ccatcgtgga catggacgag atccatccgg tgacgaccga acgcctgctc tccgcccagg
     3601 actacacccc gtttgagggg atggagctga ccggctggcc cgtacgcacc atcctgcgcg
     3661 gccggacggt cttcgccgac ggagaacccg ttggggagcc ttccggcacg tacctccagc
     3721 gtccgctcac cacggagcag ccgctcgccg cgccatagag tacggaggac ccgcacagga
     3781 tgaactatgc atctctcgtc gtggtcggcg caggcgcgtg cggcatggtc gcggcgctcg
     3841 ccgcggcgaa gcgcggggta agggttctcg ttctggagaa gggaaatgag cctgccggca
     3901 acaccgcgcg ttcgactggt ctgatccccg ccgcagggac ccgcttccag cgcgaagtgg
     3961 gcatcctcga cgattctccg gagcttatgg ccgaagacat cttcaggaag aacggctacg
     4021 agagcgaccc tgggatcaca aagctcctgt gcgagcagtc tggcccactc gtcgagtggc
     4081 tcgtagacga ggccgggtgc gagatggtct gctatacaga cttcctctat ccgggacaga
     4141 gcctccacag gatgcacggc ccgaaagacg gctacggatc ggaactcgtc caacagctgg
     4201 agaacgcaat ccgcgatgac cccaggatag agttgttcac gaacgccgct gtggacggac
     4261 tcctgtccga agacggcatg gtcacaggcg tagagacctc ggatggggag atcctggccg
     4321 atgcggtcgt cctcgcgctg aacggcttcg gcggcgaccg cgagatggtg gagaggtatc
     4381 tggggcccga ggccgcagcc gcgctctatt tcggctcgcc gaacaacacg ggcgagggca
     4441 tacggtgggg catggctctg ggagcggcga cagggcacat gggttcctac caggggcacg
     4501 cctccgtcgc cttccccgac ggacctctcg tttcctgggg tgtcgtcgtc aacggagcca
     4561 tcctcgtcaa cagggaaggc cgtcgcttcg gtttcgagat cgtcggctac tccgagtacg
     4621 caggcgcggt catggcccag cctggcgggg aggcatggga gatcttcgac gcggaggtct
     4681 atagcgcatc actcggtacg cgcttcgacg aggtcgtgga ggcgggaaag gtggtgactt
     4741 cggagactgt tgaggacctg gccgaaacct tgggtctccc cgccggtgca ctggcagaga
     4801 ctatcgagga gacgaacagg tatgcccgcg gagaggcaca ggaccccttc gggcgggacg
     4861 atattctcgg cggccctctg gaatcgccgt tctacggcat ccacgtgcgc ggggcgctgt
     4921 tccacacgca gggtggtttg agagtggatg cgagtgccag ggtgctccgc cccgacggca
     4981 cgcacataca gagtctatac gcgggtggtg ggacggccgt cggtgtcagc gggagtggat
     5041 acacaggcta ctcgagcggt aacgggcttc tggcggctgc cgtgctggga aaggtcgccg
     5101 gcgaagccgc agcagacgag ctctcgcaac gtccgacgaa gtagacattc ctttgggcaa
     5161 gcgagatccc tacccaaacc ctgatacctg aaacctgtag cctgaagcct ctgtcaggaa
     5221 actctccagg ctcactttac cctatactgg tttttcagcg gcattctggg gaaagatgct
     5281 atcagggaca caagatctcg tatgaaggag ggacatgggc gaggcttccg tagtatcggg
     5341 aaggtcatcg gtgaacgtgg atctcacggg gcgcagcgcc ctcgtcacgg gcgcggcgag
     5401 tgggatcgga cgcgcctgcg cagagaggtt ctcgcgggcc ggggcggcgg tcaccgttct
     5461 cgacctcgac ggcgatgccg cgggaagggt cgctgaaaac atcggcggcg aggcgctgca
     5521 ggctgatctc gcagactatg aggtgctcga cgatctcgag gtggacgcgg acatagtggt
     5581 caacaacgcg ggattgcaat acgttgctcc cgtcgaggaa ttcccgcccg agcgtttctc
     5641 gatgatactc agggtcatgc tcgaggcgcc gttcaggatc gtgcggaagg cgttgccggg
     5701 gatgtacgag aggggctggg gacgcgtcat caacatctca tccatccacg gcatggtcgc
     5761 ctcgccgtac aagtccgcct acatctcggc caagcacgcc ctggagggct tctccaagac
     5821 cgtcgccctc gagggagggg gcaaaggtgt cacgtccaac tgcatctgtc ccgcctacgt
     5881 caggacgccg ctcgtggaga accagatctc cgaccaggcc aggaggcacg ggatccctga
     5941 ggacgaggtg atcgagaaga tcatgctgac cgagccggcg ataaaacgcc tgatagaacc
     6001 cgaggaggtc gcggagttcg ccgccttcct ctgcgtcccc gaggcatctt tcataaacgg
     6061 cgcgtccctg gtcatggacg gcggggcgac tgcccactag acagatcgta tatagagggg
     6121 aagggatact agtgactgaa actgcggaag gaactctgct ctggcaacct tcggaggaat
     6181 tcaaggagaa cgccagaatc tccgactaca tgacgtggct gaaagaagag aaggacctct
     6241 ccttcgggga ctataacgag ctctgggagt ggtcggtcac cgacctggaa gggttctggg
     6301 gttcggtctg ggagtactgt ggcgtcgagg cctcgaaacc ctacgagagg gtgctcgcga
     6361 agcgggagat gccaggggcc gagtggttcc ccgggacgga gctcaactac gccgagcgcg
     6421 tcttgaagca cgccgcgggc cgcctggaag agcccgcgat cctgcatcag tcagaggtga
     6481 gagacctcgg cgagatgacc tggggcgagc tacaggagaa gacggccgcg ctcgccgcgg
     6541 ccctcaagga gatgggcgta cagcgcggcg acagggttgc tgcttacatt ccgaacatac
     6601 ccgaggccgt catcgccctg ctcgcctgct catccatcgg cgcggtctgg tcgagctgct
     6661 cccccgactt cggcgcgggc agcgtcgttg accgtttcaa gcagatagag cccaaggtcc
     6721 tgatcgccgt cgacggctat cgctacggcg gcaaggacta cgacaggacc caggtaatcg
     6781 cgaggttgca ggaggagatc ccgactctgc agaagaccgt ggtcctgccc tacctcacgg
     6841 aagagcctga cacgagctcg ctgcagaacg cagtaatgtg ggacgagctg ctctcccggc
     6901 acgaaggcgc tgatctcgct ttcgagcagg taccgttcga ccatccgctc tggatcctct
     6961 actcatctgg cacgacgggc ctcccgaagg ccatcgtgca cagccagggc ggcatc
//
LOCUS       DATAIR010000255         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_431455, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000255 DATAIR010000000
VERSION     DATAIR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431455"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VHM69_06125"
     CDS             <1..732
                     /locus_tag="VHM69_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX2740004.1"
                     /translation="GSRRVDVETEIEWHERQVLLRALFPVNVRSHEATFETMYGAQKR
                     PTHGNTSWDSTRFEVSAHRYADLSEPGYGVALLNDGKYGHSATGNVLGLSLLRSPMYP
                     DPLADEGDHHFTYSLFPHQGDWTEAGVVEEAFALNSPLFTAEGTQDGPHPAESGLVSV
                     EGVEVALGSLKMAEEGNGVILRLYEPHGARGECTLRFAHGVESAERVDLLEETESPVE
                     VLEGKVRLDVRPFEVVTLRLELTTQ"
     gene            765..1628
                     /locus_tag="VHM69_06130"
     CDS             765..1628
                     /locus_tag="VHM69_06130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740005.1"
                     /translation="MEHRRLGRLGRENSVLIFGGAALAESTEEAGDRAIRQALDAGVD
                     HFDTAADYGDSELHYGRWMPEIRDSIFLSTKTGLREKDPAKRQIHASLERLRVDNVDL
                     IQLHAVGDLEDLDRATGPGGSLEAAIEAKEEGLVGAIGITGHGNKAPATHVEALRRYP
                     FETVLTPWNYILSTDEAYRADYEALVEEIRRQDAGLMIIKTISRRNWPDGDQRYTTWY
                     EPFDQREHVDAAVSWVLSHDEVTGLAMVGDVTLVPMVLEAEGRRMSREEAEQVLTRAR
                     DYSSPFISMPI"
     gene            1683..>2264
                     /locus_tag="VHM69_06135"
     CDS             1683..>2264
                     /locus_tag="VHM69_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740006.1"
                     /translation="MQKVTFGKTELEITPIGFGAWAIGGTGWGGAWGHQDDDEAVGAI
                     RRAVELGINWVDTAAVYGLGHSEELVARALKGVPDSDRPYVFTKCSLVWDEKSDISNI
                     LDKNSVKRECEDSLRRLQTDVIDLYQIHWPNPDEDIEEGWSALAELKEEGKVRHIGVS
                     NFDVSQLERANGIAPVETLQPPYNMLNRDVEEEI"
BASE COUNT          468 a          699 c          738 g          359 t
ORIGIN      
        1 ggttcgcggc gcgtcgacgt ggagacggag atcgagtggc acgagcggca ggtgctgctg
       61 cgggcgctgt tccccgtgaa cgtcaggtcc cacgaggcca cgttcgagac gatgtacggt
      121 gcccagaaac ggcccacgca cggcaacacg tcgtgggact cgacccgctt cgaggtctcg
      181 gcccatcgct acgccgacct ctccgagccg ggctacggcg ttgccctgtt gaacgatggc
      241 aagtacggcc acagcgccac gggaaacgtc ctcggcttaa gcctcctgcg gagcccgatg
      301 taccctgatc cgctcgccga cgaaggagac caccacttta cctacagcct cttcccgcac
      361 cagggagact ggacggaggc tggcgtcgtg gaggaggcgt tcgcgctgaa cagccctctg
      421 tttaccgcgg aaggcacgca ggacgggccg catcctgcgg agtccgggct cgtgagcgtg
      481 gagggcgtcg aggtcgcgct cgggagcctc aagatggccg aggaggggaa cggtgtgatc
      541 cttcgcctct acgaacccca cggcgcgcgc ggcgaatgta cgctccgctt cgcccacggt
      601 gtcgagagcg ccgagcgcgt cgatctgctc gaagagacgg aaagtccagt cgaggtgctg
      661 gaagggaagg taaggctcga cgtaaggcca ttcgaggtag tcacgctgcg cctggagttg
      721 acaacacagt aagatagctt ggagagcgag gaaagggacg agggatggaa catcgtcgtt
      781 tagggcgtct tggacgcgag aactccgtgc tcatcttcgg tggggctgcg ctcgccgagt
      841 cgaccgagga ggccggagac agggccatcc ggcaggcgct cgacgctggc gtagaccact
      901 tcgatacagc ggccgactac ggggattccg agctgcacta cgggcgctgg atgcccgaga
      961 tcagggacag catcttcctc tccaccaaga cgggcttgag ggagaaggac cccgcgaagc
     1021 gacagatcca cgcctccctc gaacgcctgc gcgtggacaa cgtcgacctc atccaactcc
     1081 acgccgtagg cgacctcgaa gacctcgata gggctacagg ccctggcggc tccctcgagg
     1141 ccgccatcga ggccaaagaa gaaggactgg tcggggcaat agggatcacg ggccacggca
     1201 acaaagcccc agcgacccac gtcgaagccc tgcggcgcta cccgttcgag actgtgctta
     1261 cgccatggaa ctacatcctc tccacggacg aggcctatcg cgcggactac gaggcgctcg
     1321 tcgaagagat aaggcgccag gacgccgggc tcatgatcat caagaccatc tcgcgccgca
     1381 actggcccga tggcgaccag aggtacacca cctggtacga gcccttcgac cagcgggagc
     1441 acgtagacgc cgccgtctca tgggtgctct cccacgacga ggttacagga cttgccatgg
     1501 tcggggacgt caccctcgtc ccgatggtgc tcgaggccga ggggcgcagg atgtcgcgcg
     1561 aagaggccga acaggttctc acccgagccc gcgattactc ctcgccgttc atctcgatgc
     1621 caatctaggc ttcaggtatc aggctacagg tcttctgatg cctcaacaag aatggagaat
     1681 ccttgcagaa ggtaacgttt ggaaagacgg aacttgagat aacgcccatc ggcttcggag
     1741 cctgggcgat aggtggaacc ggctggggtg gcgcgtgggg ccatcaggac gatgacgagg
     1801 ccgtcggcgc gattcgccgt gccgtcgagc tcggcatcaa ctgggtcgac accgccgccg
     1861 tctacgggct cggtcactcg gaggagctcg tagccagggc gctcaagggt gtccccgact
     1921 ccgaccgtcc ttacgtattc accaagtgct cattggtatg ggacgagaaa agcgacatct
     1981 cgaacatact cgacaagaat tccgtcaagc gcgagtgcga ggacagcctc aggcgtctgc
     2041 agacggacgt catcgatctg taccagatcc actggcccaa ccctgacgag gacatcgagg
     2101 aggggtggag cgcgctcgcc gagctcaagg aggaaggcaa ggtccgccac atcggtgtct
     2161 ccaacttcga cgtctcccag ttggaacggg cgaacggtat tgcccctgtc gagacgctcc
     2221 agccaccgta caacatgctg aaccgtgacg tcgaggagga gata
//
LOCUS       DATAIR010000256         4800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_238333, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000256 DATAIR010000000
VERSION     DATAIR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238333"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VHM69_06140"
     CDS             complement(<1..279)
                     /locus_tag="VHM69_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2740007.1"
                     /translation="MFLSLFQENPSVAVAFLLGLVVGITVHEASHATSAYLLGDDTAY
                     RDGRVTLNPVSHLDVLGSLMLLMAGFGWGRPTPVVPSKLRGGVFGPVAV"
     gene            complement(463..1239)
                     /locus_tag="VHM69_06145"
     CDS             complement(463..1239)
                     /locus_tag="VHM69_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740008.1"
                     /translation="MQPGRPGHRAGSPVGNFIELREARKAKRDEVYRRRRLMAVSLLV
                     LGAVALVFAVFVQTKASDTAERAVPIDPNNAGPDTVLAEAARVSISTPIRPASLTGLG
                     YHPEGESLAVMEPRGKNLSANALVGLLSRGETPERIHYYVMDAAGRSGPQTGALDVGA
                     GTGTTVYTPVTGTVTAIRPDPMVQNANVIEIKPDANPDVRVNVSLVRSDGEAGVNDYV
                     TAGITELGTVADSAEVLDPQLSSYTTDAGNHVTVSVSKGG"
     gene            1427..2602
                     /locus_tag="VHM69_06150"
     CDS             1427..2602
                     /locus_tag="VHM69_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2740009.1"
                     /translation="MSTRKLVISTYFQYIVIAVGVVLLVSFVRQVSGVLLTFLAAGVL
                     AYALNPVVRRLEGMRVPRALAVVGVFLVLVLVVIVALLLIIIPTIEQIQTLVRNPQII
                     ARQATSLTEQAQRLPYVGVYVVELDQDRILQLIRDNAPSAGQVFSVATGVIGGVFGVF
                     GTLFNLLLMVLISIYLLLEREKIPRALLGAIPETVRDHFVELFFAVEQALVRYLKGQF
                     FLCAIMGVIGWGIMFFTVGEYALLVGAWVAATEIIPVLGAFLGAVPAVGIALFVPDGG
                     FIPALVVAGLFLVAQQVEGNILVPRIQGGSTGVHPLWVLFATLAGTALYGIIGAIFAV
                     PIVAIVAETLRYLRRTLVFESWANPPIRPADEEPAELAAASPSKAAGVGRTDEKEDR"
     gene            2602..3216
                     /locus_tag="VHM69_06155"
     CDS             2602..3216
                     /locus_tag="VHM69_06155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2740010.1"
                     /translation="MTIPHRGPRKPASFRVASKDALLFVLHPLVRWLSVMRVRPDTLT
                     VIGWTLAVFSAVLFAMGYARIAGVVMLLGGLFDALDGAVARESNRMSSFGAFLDSTLD
                     RLSESTIFVGLVFFYASANRPYGALLAGTAMTFSLLTSYARARAEGLNIDCEVGLLER
                     AGRIVILSVLSIVGLPTVGLVLVAAGAIVTTAQRILHVRRATRG"
     gene            3200..3721
                     /locus_tag="VHM69_06160"
     CDS             3200..3721
                     /locus_tag="VHM69_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740011.1"
                     /translation="MRPAGREERTRDAAGDYREKTAYEISRISRSMKEAGHPAPLGDP
                     TSGVVLVVEQPVGPRALEALERSLHAVGLPDAYVTYESTGLLAEEMQATEPHALIAIG
                     AGAARDIDAIDYPLVRNPFSEAEPGIWFAWTKGTAGLVLPALAPALDDDATKRRFWRA
                     FLTLRDLASPTRP"
     gene            4051..>4800
                     /locus_tag="VHM69_06165"
     CDS             4051..>4800
                     /locus_tag="VHM69_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740012.1"
                     /translation="MVVILGLAIALIFVTGISAGAEPEGGIRAKKADVAAAQDRLMEI
                     RMEASSAQASYNNALYEMNQLNGQITKATEDLDAAKERLAEAKKSLEERASQVYKSGN
                     VAFMDVLVGVDNFSEFASRLDLWVRLLAQERAEFIEVREAKNELASRKDELEAQRKQR
                     VKAVERAIAQKERAIKAEAQAEAYLNSLNAELRAEIQAAQDRQAERARAAAAAMAEEK
                     PASEPEPEPIPEVQVSEVKRVPVPQPDLESEQ"
BASE COUNT          846 a         1486 c         1533 g          935 t
ORIGIN      
        1 gacggccacg gggccgaata ccccgcccct cagcttggac ggcaccacgg gcgttggcct
       61 gccccatccg aagcccgcca tgaggagcat caggctgcca agcacgtcga ggtgcgagac
      121 ggggttgagg gtcaccctcc cgtcacgata ggccgtatcg tcgccaagta ggtaggccga
      181 ggtcgcgtgg gacgcctcgt gtaccgttat gcccacgacg aggccgagga ggaaggccac
      241 ggcgacggac gggttctctt ggaacaggct taggaacatg gatgctcgta tgatacacgc
      301 cagccgggac gatggagcat ggccagcagt cggactggtc taaagcgttg tgcaatgtag
      361 ctgaatgctc cttgcagacg cagtcagctt ctcaccaaga acaaagaagc tgacggctga
      421 tacctgatgg ctgacagcgc gattagcaga aggccaatcg ccttaaccgc ccttcgagac
      481 cgagacggtg acgtggttgc ccgcgtccgt ggtgtaagag gagagctggg gatccaggac
      541 ctccgccgag tcggccacgg tgccgagctc ggttattccg gccgtcacgt agtcgttgac
      601 gcctgcttcg ccgtcgctgc gtaccaggga gacgttgacg cggacgtccg gattggcgtc
      661 cggttttatc tctatgacgt tggcgttctg aaccattggg tcgggccgga tggccgttac
      721 cgtgccggtc acaggcgtgt agacggtggt gccagtccct gcgccgacgt ctagggcgcc
      781 ggtctgggga ccgctccgcc ccgcggcgtc catgacgtag tagtggatcc tctccggggt
      841 ctctcccctg gagaggaggc cgacgagcgc gttcgccgag aggttcttgc cccgcggttc
      901 catcacggcc aggctctcgc cctcggggtg atagccgagc cctgtgaggc tggcagggcg
      961 gatcggggtg gagatgctga ccctggcggc ctcagcgagc actgtgtctg gacccgcgtt
     1021 gttcgggtcg atggggaccg cgcgctcggc ggtgtccgaa gccttggtct ggacgaacac
     1081 agcgaagacc agcgcgacag cgccaagcac cagcagggaa accgccatca ggcgccgcct
     1141 ccggtagact tcgtcccgct tcgcctttct ggcctcgcgc agctcgatga agttgcctac
     1201 cgggcttccg gcgcgatggc ccggccggcc cggttgcata gctcttccgg tggtccgtcg
     1261 aggtcccggc gtttcccacc gctccccaac atgttgggct ctctgaacat ttccccctct
     1321 catactgggc gaagattata cagaggggtt tggggagtgt aaactcgcgc gtcgcttcgt
     1381 tcggggctgg tatatttcgt atcgtagaga tcagatcgga gaccgggtgt ccaccagaaa
     1441 actggtcatt tcgacatact tccagtacat cgtgattgcc gtgggtgtgg tgctgctcgt
     1501 gtccttcgta cgccaggtaa gcggcgtcct gctcaccttc ctcgccgccg gcgtccttgc
     1561 ctacgccttg aaccccgtcg tgcgccgcct cgaagggatg cgtgtgccga gggctctcgc
     1621 cgtggtcggg gtgtttctgg tccttgtcct cgtggtaatt gtggcgctgc ttttgatcat
     1681 catcccgacg atagaacaga tacagaccct ggtccgtaac ccccagatca tcgcgcgcca
     1741 ggcgacgtcg cttacggagc aggcccagag gcttccctac gtcggcgtgt acgtggtgga
     1801 gctggatcag gacaggatct tgcaactgat cagggacaac gcgccgtcgg ccgggcaggt
     1861 cttcagcgtg gctactggcg tcatcggggg ggtgttcggg gtcttcggga ccctgttcaa
     1921 cttgcttctc atggtactta tctccatcta tttgctgctg gagagggaga agataccgcg
     1981 tgcactgctc ggcgcgatac cggagacggt gcgcgaccac ttcgtcgaac tgttcttcgc
     2041 cgtcgagcag gcgcttgtca ggtacttgaa gggacagttc tttctgtgcg cgatcatggg
     2101 cgttatcggg tgggggataa tgttcttcac cgtcggggag tacgccctcc tggtcggggc
     2161 ctgggtggcc gccacggaga tcatccccgt cctcggggcc ttcctcgggg cggtgcctgc
     2221 ggtcgggatc gccctcttcg tccccgacgg gggctttatc ccggccctcg tcgtcgccgg
     2281 tctcttcctg gtcgcccagc aggttgaggg gaacattctg gtgccgagga tccagggcgg
     2341 ctcgaccggc gtgcatcctc tctgggtgct cttcgccacg cttgccggca cggctctgta
     2401 cgggatcatc ggcgcgatct ttgccgtccc gatcgtcgcg atcgttgcgg agactctgcg
     2461 ctacctgcgc aggacgctgg tcttcgagag ctgggcgaac ccgcccatca ggccggccga
     2521 cgaggagcct gccgaacttg cggcggcctc gccctcgaag gcggccggtg taggacgcac
     2581 cgacgagaag gaggaccgct gatgaccatc cctcaccgcg gaccccgcaa gcccgcttct
     2641 tttagagtgg cctcgaagga tgcgctcctc ttcgtcctgc atccgctcgt gcggtggctc
     2701 tccgtcatgc gggtgcgtcc cgatacgctc acggttatcg ggtggactct ggcggttttc
     2761 tccgccgtcc tgttcgccat gggatacgcg aggatcgccg gtgtggtgat gctccttgga
     2821 ggacttttcg acgcgctcga cggcgcggtc gcccgtgagt cgaaccgtat gagctccttc
     2881 ggggcctttc tcgactcgac cctggaccgg ctctccgagt cgacgatctt cgtcgggctc
     2941 gtgttcttct acgccagcgc gaaccgtccc tatggagccc ttctggcagg taccgccatg
     3001 accttctcgc tactcacctc ctacgcccgc gcccgcgcgg agggtctcaa catagactgc
     3061 gaggtcgggc tcctggagcg cgcggggagg atagtgatcc tctctgtcct ctcgatcgtc
     3121 ggcctgccca ccgtcggcct cgtactcgtc gcggcaggcg ccatcgtaac cacagcccag
     3181 cgcatcctcc acgtccgccg tgcgacccgc gggtagggag gagcggaccc gcgacgcggc
     3241 gggcgactat agggaaaaaa cggcgtacga gatctcccgc atctcccgca gcatgaaaga
     3301 ggcaggacac cccgccccgc tcggcgatcc cacctcaggc gtcgtgctgg tcgtcgagca
     3361 gcctgtaggt ccccgcgccc tggaggccct ggaacgcagc ctgcatgccg tcggactgcc
     3421 tgacgcctac gtgacctacg agtcaaccgg cctgctcgct gaggaaatgc aggccaccga
     3481 gccccacgcc ctcatcgcaa taggcgcagg cgcagcccgc gatatcgacg ccatcgacta
     3541 cccactggta aggaatccgt tctccgaagc cgaaccaggc atctggttcg cctggacaaa
     3601 gggcacggca ggcctcgtcc ttcccgccct cgcccccgcg ctggacgacg acgcaaccaa
     3661 gcgacgcttc tggcgggcct tcctgaccct gagagacctc gcgtccccta cacggcctta
     3721 gaagaatacg aagatcccac ccggacaagg ggcgaggtca agtccacagc gcattgcagt
     3781 ttgtttctct atctacaaac gcttttcaga tacgttacaa atattaaatt ttgttaaaaa
     3841 ttcatttcgt gtaggaaaca cggatctccc atatttaagc cactttcata aacagaggag
     3901 aacgctggta acagaagtgc atattgcgcg gcgtaagagc ctcttctaaa gtgccggact
     3961 taggaggggg gattcttcct aacaggtccc cttacagagt ttatataacc tactggaaag
     4021 gggcctgtac ctatgcctcg aagggcgagt ttggtcgtaa ttcttggttt ggctattgcg
     4081 ctcatattcg tgacgggcat ctcagcaggg gctgagcctg aaggcggcat acgggcgaaa
     4141 aaggctgatg tcgctgcggc ccaggaccgt ttgatggaga tacgcatgga ggcgagttcg
     4201 gcccaggcgt cctacaacaa cgcgctctac gagatgaacc agttaaacgg gcagataacg
     4261 aaggcgacgg aagatctcga cgccgccaag gagcggctcg ccgaggccaa aaagagcctg
     4321 gaggagcgtg cttcgcaggt ttacaagagt gggaacgtag ccttcatgga cgtgctcgtg
     4381 ggggtagaca acttctccga gttcgcatcg aggctggacc tttgggtgag gttgctagcc
     4441 caggagaggg ccgagttcat tgaggtccgc gaggccaaga acgagctggc gtcgcgcaag
     4501 gacgagctcg aggcccagcg caagcagagg gtcaaagctg tcgagagggc cattgcccag
     4561 aaagagcgtg ccatcaaggc cgaggcgcag gccgaggctt atctgaactc cctcaacgcc
     4621 gagctcaggg ccgagatcca ggccgcccag gaccggcagg ccgagcgggc ccgcgccgca
     4681 gcagctgcga tggccgagga gaaaccggct tctgagccag agccagagcc catacccgag
     4741 gttcaggttt cagaggtcaa gcgggttccc gtgccgcagc ccgacctgga gtccgagcag
//
LOCUS       DATAIR010000257         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_330342, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000257 DATAIR010000000
VERSION     DATAIR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330342"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1502)
                     /gene="acs"
                     /locus_tag="VHM69_06170"
     CDS             complement(<1..>1502)
                     /gene="acs"
                     /locus_tag="VHM69_06170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2740013.1"
                     /translation="EQGRGDKTAILWEGDEPGDSRTFTYSELKAEVEKFANVLKGLGV
                     EKGDPVAIYLPMIPELPMAMLACARIGAPHSVVFGAFSADSLRDRINDCEAKVLVTAD
                     GGPRGGKVTPLKANAEEALQDTPSIESVVVAQRSGEDVPMTEGRDHFWNDLMADADAE
                     CPAEEMDSEDILYILYSSGSTGKPKGIVHTTGGYLTQVKATTRWVLDLKEDDVYWCQA
                     DIGWVTGHSYLVYGPLSNGATTVQFEGTPSYPGPDRHWDVIERHGVTIYYSAPTVIRA
                     FMKQGTEPLEKHDLSSLRLLGTVGEPINPRAWEWYQENVGGGRCPVVDTWWQTETGAI
                     MIAPLPGIVPTKPGSATRPFPGIFVDIYDEDDKPIEGPGKGNLVITRPWPGMLRTIYK
                     DPERYRKTYWERLGDKYFVEDGAERDEDGYYTITGRIDDVMNVSGHRIGTMEVESALV
                     SHQGVAEAAVIGRHDEDKGQAIVAYVILEGDREGTDDLIKELNDQVRKAI"
BASE COUNT          231 a          498 c          468 g          305 t
ORIGIN      
        1 ctatggcctt cctgacctgg tcgttgagct ccttgatgag gtcgtcggtt ccctctctgt
       61 cgccctcgag gatcacgtag gcgacgatgg cctgaccctt gtcctcgtcg tgacgcccta
      121 taacggcggc ctcggccaca ccctggtggg agacgagcgc gctctcgacc tccatcgtgc
      181 cgatgcggtg acccgagacg ttcatcacgt cgtctattct gcctgtgatg gtgtagtaac
      241 catcctcatc gcgctcggcg ccgtcctcga cgaagtactt gtcgccgagt ctctcccagt
      301 aggtcttgcg gtagcgctcg gggtccttgt agatcgtgcg tagcatgccc ggccagggcc
      361 tggttatgac caggttcccc tttccaggac cctcgatggg cttgtcgtcc tcgtcataga
      421 tatcaacgaa gattccgggg aaaggccggg tggcgctgcc aggcttggtc gggacgatgc
      481 cggggagagg cgcgatcatg atcgcgcccg tctccgtctg ccaccacgta tccacaacgg
      541 ggcaacgtcc gcctccgacg ttctcctggt accactccca cgcccgcggg ttgatgggct
      601 cgccgacagt cccgagcagc cgcaaggacg agaggtcgtg cttctccagc ggctcggtgc
      661 cctgcttcat gaacgcccgg atgacggtcg gggcggagta gtagatcgtt accccgtgcc
      721 gctcgatgac gtcccagtga cggtccggac cgggatagct aggcgtcccc tcgaactgca
      781 ctgtggtggc accgttgctc aagggcccgt agacgagata ggaatgtccc gtgacccagc
      841 cgatgtcggc ctggcaccag tagacgtcgt cctccttgag gtcgagcacc cacctggtcg
      901 ttgccttgac ctgcgtcagg tagccgcccg tggtgtggac gatccccttc ggcttgcccg
      961 tcgagcccga ggagtagagg atgtagagga tgtcctccga gtccatctcc tccgcgggac
     1021 actcggcgtc ggcgtcggcc atcaggtcgt tccagaagtg gtcgcgcccc tcggtcatgg
     1081 gcacgtcctc gccgctgcgc tgagccacga cgacgctctc gatggacggc gtgtcctgga
     1141 gagcctcttc ggcgttcgcc ttcaaaggcg tcaccttgcc gccacgcggc ccgccgtctg
     1201 cggtgacgag taccttcgcc tcgcagtcgt tgatgcggtc gcgcagagaa tcggccgaga
     1261 atgccccgaa gacgacagag tgcggagccc ctatgcgggc gcacgcgagc atcgccatcg
     1321 gcaactcggg gatcatcgga aggtagatcg cgaccggatc ccccttctct acgccgagac
     1381 ctttgaggac gttggcgaac ttctcgacct cggccttcag ctcggagtag gtgaaggtcc
     1441 tgctgtcacc gggctcgtca ccctcccaga ggatagcggt cttgtcgccc ctgccctgct
     1501 ct
//
LOCUS       DATAIR010000258         5261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_330346, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000258 DATAIR010000000
VERSION     DATAIR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330346"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VHM69_06175"
     CDS             complement(<1..1318)
                     /locus_tag="VHM69_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2740014.1"
                     /translation="MSESGPASIAEAFVRNAERAPGRLCLRFEGEEISYKSMRERAER
                     FGAALEAWGLRPGERVALFLGNQPDLLAAYLGTHLAGGVVVPVNTQYRRGELRHIFGD
                     AGVRLCLTDGERKPELDRADLPELEALIEAGPQLEDFLGSGDTLQSGMPGGDDLAVIA
                     YTSGTTGSSKGAMLLHGNLVANAEAVCAAWRWTDQDMLLLALPLFHTHGLMVGAHGTF
                     FTGAGAELHRKFDAAEAYDALLEGRVTMFFGVPTMYTRLLREAATRDERPRPIRLYVS
                     GSAPLSPQAFDEFESIFGERILERYGMTETIMNLTNPYEGERRPGTVGRPFPGQEARV
                     VGVKTHEPLPEGEIGEIEVRGPHVFPGYWNRPNATDESFDDEGWFATGDLGSVGEGGY
                     ITISGRAKELIISGGYNVYPREVEEAIEGCPGVSDVAVVGLPDPEFG"
     gene            1649..3091
                     /locus_tag="VHM69_06180"
     CDS             1649..3091
                     /locus_tag="VHM69_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2740015.1"
                     /translation="MTGAGLAGASLLGAAGCGGSASSGSSGGGMWKQFAGTTLNFVSE
                     NTAPTTAIAANLNPFRELTGINVNVLQLELGALVQKVALDIGSGEGAYQVVYADPYQI
                     MAPYHEALVDLNEFNDDDNLPSIPKGLDDFIPTQLDAAGRFENEKNLYAIPYDCPTMI
                     WMYRMDLFDKYGDQMKQDLGFDPTPSPSVTWEQYYQIAKWFNENQDEVPYGTGHQARQ
                     YDSLMCDFSNILWAYGGDYFDNDTVGSLGTATPGPCTLDEPEALEAAAFYKKLIDIAH
                     PGSVSWDWNATDEAFRTGQTAMTPNWHEFAAVDQDPAESTIVGKIGYSPLPRGPVRNA
                     NMWGGTGVGINGASPIEEQKAAWLFLVWATSPETMLMGMKSDVGGGTPTRGSLYKLPE
                     VEKAMEPPSDMPNLLTYDAVSEAWQPENIGLRPKIQSWNECDTAIYTEVSKMLAGQKS
                     PEEAMKTAKVYFDEAQTRVKNLASRPRGVA"
     gene            3092..4003
                     /locus_tag="VHM69_06185"
     CDS             3092..4003
                     /locus_tag="VHM69_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2740016.1"
                     /translation="MAATQGSGTSNDKSAGGLSLEMKMLLPVLLLLAVLSLFPFFYII
                     WMSLNTVSLINGISFDFAGLTNWTRMFTDVVVGAAWVRSVIYFVATVGLEMILGIAIA
                     LLVHELVWGKNLVLSLILLPMFMAPVIVGLLGRFLTDSSYGLYAFVLRETGIFESDIL
                     GTSSSALAAVILMDVWEWTPLIVLIVLAGLASVNQYVIEASKVDGANYIERLRHIVAP
                     SIMGVVIVALLVRSMDAIRYFDIITVTTNGGPGNTTKIIPIRLYETAFRFFDLGYAAA
                     IGLGMLVFSILVATVFVRILESRGLAK"
     gene            4000..4842
                     /locus_tag="VHM69_06190"
     CDS             4000..4842
                     /locus_tag="VHM69_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2740017.1"
                     /translation="MTTPSSTRGTGKQAVLYAILVLVLLWTLVPIVWMVLSSFKPGVA
                     QLSPTPVFIFKPTMQHYVALFTGGNLGTYFLNSVLAAGISTIIAVSLGCLAGYGLARS
                     HFRGKRHVAFWIISTRMAPIAAVVLPLFILFRYVHLLDSIPGLIVAYLTFNLPFAIWI
                     MNAFFADLPPSLEEAAMVDGASRFQAFYRIALPLVTPGIVTTAILCLVFSWNDYAFAA
                     TFAGPNSQTLPIAAAQLNTQTGLSWGQLSAIGTIVVLPMIIVGLAVRRYLVRGLTLGA
                     VTGE"
     gene            4857..>5261
                     /locus_tag="VHM69_06195"
     CDS             4857..>5261
                     /locus_tag="VHM69_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2740018.1"
                     /translation="MHNRAAVMYGTHDVRLEEVPVPEPGPKEVLVEVRAVGVCGSDVH
                     YYEEGRIGSFVVREPLILGHESMGTVVALGEGATKHEVGERVTLEPGVPDGTCRECRA
                     GRYNLCPNVRFFATPPIDGAFTNYVSIHEDFAY"
BASE COUNT          966 a         1710 c         1597 g          988 t
ORIGIN      
        1 ctccgaactc cgggtccggc aggccgacga cggcgacatc ggagacgccc gggcagcctt
       61 cgatcgcctc ctccacctcg cgggggtaga cgttgtagcc gccgctgatt attagttctt
      121 tggcccgccc gctgatggtg atgtagccac cctcgccgac ggatccgagg tcccctgtcg
      181 cgaaccagcc ctcgtcgtcg aagctctcat cggtggcgtt gggcctgttc cagtagcccg
      241 ggaagacgtg cggcccgcgg acttcgatct cccctatctc cccttcaggc aagggctcgt
      301 gtgtcttcac gccaacgacc cgcgcctcct ggcctgggaa gggccgcccc accgtaccgg
      361 gcctgcgctc gccttcgtat gggttggtga ggttcatgat cgtctcggtc atgccgtatc
      421 gttcgaggat tcgctccccg aagatgctct cgaactcgtc gaacgcctgg ggacttagag
      481 gcgcgctgcc tgagacatac agtcggatcg gccgcgggcg ctcatcgcgg gtcgcggcct
      541 cccgcagaag acgcgtgtac atggtcggga ccccgaagaa catcgtgacc ctcccctcca
      601 gaagggcatc gtacgcctca gccgcgtcga acttcctgtg cagttcggcc ccggcaccgg
      661 taaagaaagt gccgtgcgcc ccgaccatga gaccgtgggt gtggaaaagc ggcagggcaa
      721 gaagcaacat atcctggtcg gtccaacgcc aggccgcgca caccgcttcc gcgttggcga
      781 cgaggttgcc gtgcagcagc atcgccccct tgctgctgcc cgtcgtcccc gaggtgtaag
      841 cgatgacagc caggtcatca ccgcccggca tccctgactg aagggtgtca ccagagccga
      901 ggaagtcttc gagctggggg ccggcttcga tcaacgcctc cagctccggc aggtccgcgc
      961 gatcgagctc gggcttgcgt tccccgtcgg tgaggcagag acgtaccccg gcgtccccga
     1021 atatgtgccg cagctcgccc ctgcggtact gggtattcac ggggaccacg acgcctccgg
     1081 ccaggtgtgt tccgagatag gcggccagca ggtcgggctg gttgccgagg aagagcgcga
     1141 cgcgctcgcc gggtcgaagt ccccaggctt ccagggcggc accgaacctt tccgcccgct
     1201 cgcgcatgct cttgtaggag atctcctccc cctcgaagcg caggcacagc ctcccgggcg
     1261 cgcgctccgc gttgcggacg aacgcctcgg cgatcgaagc cggaccgctc tcgctcatgg
     1321 gcatcctttc agctttccgt ggccgatgcg ctcaaagaag ggtacatccg cgcgcgacgc
     1381 tcgcgcaacg ggcgccggcc ggatccaaac ttgttgacac ggattggcgc aagctctatc
     1441 ctcgatcagg acaccgggga tagcaggggt gctcttctgg gagatacggc gacgaagaag
     1501 tcaggcgggg gcgttatcat gacacgcccg tttacgcctg ttacgtggct tgcatctact
     1561 cctgccgtga ttcgcggccc ggcgctactg gggcggagga gggggttttc tgatgcggaa
     1621 gacatattct cggagaggct ttctaaagat gaccggcgcc ggtctggccg gcgcctcgct
     1681 gctcggcgcc gcggggtgcg gcgggtcggc gtcttcaggc tcctccggag gcggtatgtg
     1741 gaagcagttc gcgggaacga cgctcaactt cgtctccgag aacaccgccc ccaccacggc
     1801 tatagccgcg aacctgaacc cgtttagaga gctcaccggg atcaacgtca acgtactgca
     1861 gctcgagctc ggtgcgctcg tacagaaagt cgcgctggat attggatcgg gagaaggagc
     1921 ctaccaggtc gtctacgccg acccttacca gatcatggcc ccctaccatg aggcgctcgt
     1981 cgacctcaac gagttcaacg acgacgacaa tttaccctcg atccccaaag gcctcgacga
     2041 cttcatcccg acccagctcg atgcggcggg ccgcttcgag aacgagaaga acctctacgc
     2101 tatcccctac gactgtccga ccatgatctg gatgtaccgc atggacctct tcgataagta
     2161 cggcgaccag atgaaacagg acctcggctt cgacccgacg ccttcgccat ccgtgacgtg
     2221 ggagcagtac taccaaatcg caaagtggtt caacgagaat caggacgagg ttccctacgg
     2281 tacgggccac caggcccgcc agtacgactc gttgatgtgc gatttctcca acatcctgtg
     2341 ggcttacggc ggcgactact tcgataacga cacggtcggc agcctgggga ccgcgacccc
     2401 tggcccttgc acgctggatg agcctgaggc gcttgaggcc gcggcgttct acaagaagct
     2461 catagacata gcccatccag gtagcgtctc ctgggactgg aacgccaccg acgaggcgtt
     2521 caggacgggc cagacagcca tgacacccaa ctggcacgag ttcgccgccg tggaccagga
     2581 cccggcggag tcgacgatcg tggggaagat cgggtactcc ccgctcccca gggggcccgt
     2641 gaggaacgcc aatatgtggg gcgggacggg cgttggcata aacggggctt cgcccatcga
     2701 ggaacaaaag gccgcgtggc tcttccttgt gtgggcgaca tctcccgaaa cgatgctcat
     2761 gggcatgaag agcgacgtcg ggggcggtac cccgacgcgg gggtctctct acaagctacc
     2821 ggaggtggag aaggcgatgg aaccgccgtc agacatgccg aacctgctca cctacgacgc
     2881 cgtctccgag gcctggcaac ccgagaacat cggcctcagg cccaagatcc aatcctggaa
     2941 cgagtgcgac accgccatct acaccgaggt ctccaagatg ctcgcaggcc agaaatcccc
     3001 cgaggaggcg atgaagacgg cgaaggtgta cttcgacgag gcacagacga gggtgaaaaa
     3061 cctcgcctca aggccaaggg gggtcgcgta gatggcagcc acacaaggaa gcggaacctc
     3121 aaacgataag agtgcaggcg gactctcgct cgagatgaag atgctcctgc ccgtcctcct
     3181 gttgctcgcc gtactctcgc tcttcccgtt cttctacatc atctggatga gcctgaacac
     3241 ggtctcgctc atcaacggca tctccttcga cttcgcaggg ctaacgaact ggacccgcat
     3301 gttcaccgac gttgtcgtcg gggcggcatg ggtccggtcc gtgatctact tcgtcgccac
     3361 cgttggcctg gagatgatcc tcggtatcgc catcgccttg ctcgtgcacg agctggtgtg
     3421 ggggaagaat ctggtacttt cgttgatttt gctgccgatg ttcatggcgc ccgtcatcgt
     3481 ggggctcctc gggcgcttcc tcaccgactc ctcctatgga ctctatgctt tcgtcctgcg
     3541 cgagacgggc atcttcgaga gcgatatttt gggcaccagc agctccgccc tggccgccgt
     3601 catcctgatg gacgtctggg agtggacgcc tttgatcgtc ctgatcgtgc tcgccggcct
     3661 cgcctccgtg aaccagtatg tgatcgaagc ctccaaagtg gacggcgcga actacatcga
     3721 gaggctacgc cacatcgtcg cgccctcaat aatgggcgtg gtcatcgtgg ctttgctcgt
     3781 ccgctcgatg gacgccatac gctacttcga catcatcacg gttacgacca acggaggacc
     3841 cggcaacacg accaagatca tccctatcag actgtatgag acggcgttcc gctttttcga
     3901 cctcggttac gccgccgcca tcgggctcgg gatgctggtg ttctcgatcc tggtcgccac
     3961 ggtgttcgtc aggatcctcg agagcagggg gctagcgaaa tgaccactcc ttcttcaacc
     4021 cgaggcacgg gcaagcaagc cgtactctac gccatattag tactcgtcct gctgtggacg
     4081 ctggtcccga tagtgtggat ggtcctctcc tcgttcaagc caggcgtggc ccagctctcg
     4141 ccgacgcccg tgttcatatt caagcccact atgcaacact acgtggcgct cttcacggga
     4201 ggaaacctcg ggacctactt cctcaacagc gtactcgcgg cgggcatctc gacgatcata
     4261 gcggtatctc taggctgtct cgccggttac gggctcgcga ggagccattt caggggcaag
     4321 cggcacgtgg cgttctggat catctcgaca cgcatggccc cgatagccgc cgtggtactg
     4381 ccgctcttca tcctgttccg ctatgtacac ctgctcgact cgatccccgg ccttatcgtc
     4441 gcctacctga cgttcaacct gcctttcgcg atctggatca tgaacgcctt cttcgctgac
     4501 ctgccccctt cgctggaaga ggcggcgatg gtcgacggcg cttcgaggtt ccaggcgttc
     4561 taccgcatcg ctctgccgct ggtcacgccc ggcatagtga cgacggccat cctctgcctg
     4621 gtcttctcgt ggaacgacta cgccttcgcc gcaaccttcg ccggaccgaa cagccagacc
     4681 ctccctatcg ccgcagcgca gctgaacacg cagacgggcc tgagctgggg ccagctctcc
     4741 gccataggca cgatagtcgt gctccccatg ataatcgtcg ggctggccgt gagacgctac
     4801 ctcgtacgag gcctcaccct cggcgcggtc acgggggagt aagagaaagg aacctcatgc
     4861 acaacagggc agcggtcatg tacgggacgc acgacgtcag gctcgaagag gtaccagttc
     4921 cggagcctgg cccgaaggag gttttggtag aggtcagggc cgtaggggtc tgcggttccg
     4981 acgttcatta ctacgaggag ggacgaatcg gctcgttcgt cgtgagggag cctttgatcc
     5041 tgggccacga gtcgatgggt acggtcgtcg ctctgggaga aggcgcgacg aagcacgagg
     5101 tcggagaacg cgtcaccctc gaacctggtg tccccgacgg gacctgccgc gagtgccggg
     5161 cgggccgcta caacctgtgt ccgaacgtgc ggttcttcgc cacgcccccc atcgacggcg
     5221 cattcaccaa ctacgtcagc atccacgagg acttcgcata c
//
LOCUS       DATAIR010000259         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_431640, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000259 DATAIR010000000
VERSION     DATAIR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431640"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHM69_06200"
     CDS             <1..201
                     /locus_tag="VHM69_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2740019.1"
                     /translation="LLRETRGDDFAGSVRILYGGSVKPNNAAEIMGQQDIDGALVGGA
                     SLEVESFVELVEAAKNSVAKSE"
     gene            226..453
                     /gene="secG"
                     /locus_tag="VHM69_06205"
     CDS             226..453
                     /gene="secG"
                     /locus_tag="VHM69_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2740020.1"
                     /translation="MLTYIIAFFHVVFCVALVGLILLHSGKGGGLSSSLGGGMGGTFS
                     GTTIMEKNLTRLTLIVVGLFLLTNISLYFLG"
     gene            complement(727..814)
                     /locus_tag="VHM69_06210"
     tRNA            complement(727..814)
                     /locus_tag="VHM69_06210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(778..780),aa:Leu,seq:gag)
     gene            890..1732
                     /locus_tag="VHM69_06215"
     CDS             890..1732
                     /locus_tag="VHM69_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2740021.1"
                     /translation="MGRKWFVRAVLAGLACFVALTLRKGGHRTIEGGWPVNLAHRGAS
                     SLAPENTLEAFRMAVGAGAGGLELDVHLTRDGYVVVIHDATVDRTTSGTGAVAEMTLD
                     ELRGLDAGHGQYGDRSLRVPTLAEVLQEFPGVPINIDIKKAHPGVEEAVLGVLRDAGA
                     EGRILVASTPHGIISRFRKLSGGRVSTGASKWEIAVFYILSRLHLERIIRPAYDALQV
                     PLRHRGIPLVTRRFVEAAHARGVRVDVWTINQAGEMRRLLDLGADVIMTDRPETLAGV
                     LRER"
     gene            complement(1734..2033)
                     /locus_tag="VHM69_06220"
     CDS             complement(1734..2033)
                     /locus_tag="VHM69_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740022.1"
                     /translation="MSYVTIQVIWLLCIFITPPALKLYPSKLWAVPFCMVTVANGLHM
                     IFFRYEYNAVLRRAARILPYAQYLSPLREDPRYFLPLGTAYTLFGVASIVLLIAA"
     gene            2192..3442
                     /locus_tag="VHM69_06225"
     CDS             2192..3442
                     /locus_tag="VHM69_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2740023.1"
                     /translation="MIQESLIKDPSLAPEGHRKIDWVAQHAPVLNKIAGDQLADGSLR
                     GRKIAMTIHLEAKTAYLAILLSEAGAEVTVSGSNPLSTQDDVCAALAERGVRVFATHD
                     PSDEDFEQYLHRTIETGPDLIVDDGAELVGRLIEHHPDLVGKVIGASEETTTGVLKLK
                     AMTAEGVLPFPVLAANDARMKHLFDNRYGTGHSSIVALLSNTNLFLSGKAVVVMGFGW
                     VGRGLAKYADGMGARVIVCEPDPVKLLEAYSEGYEVMNSLQAAEVGDVFLTGTGNLKV
                     LGSEHFARMKDGALLTNAGHYDHEFDLAALRETAAQSREVRRNITEYELPDGRRLHVL
                     ARGRLVNIAAADGHPVEIMDLTFAVQALAAHHLANHAGEIAPGIQPIPEEIDDLVARA
                     KLASLGVEPETLSEEQISYQQSWR"
BASE COUNT          704 a         1032 c         1127 g          603 t
ORIGIN      
        1 ctattgaggg agacgcgggg agacgacttc gcaggcagcg tccgtatact ttacggcggc
       61 tccgtcaagc cgaataacgc ggccgagatc atgggccagc aggatatcga cggggcactg
      121 gtcggcgggg cgagcctcga agtcgagtcg ttcgtcgaac tggtcgaggc ggcgaagaac
      181 tccgtcgcca agtcggaata gccgagcgtg tggtaaggta ggcacatgct gacttacatc
      241 atagctttct ttcacgtcgt gttctgtgta gccctggtcg gcctgatcct tctccactca
      301 ggtaaggggg gcgggctatc ctcgtcgctc ggaggcggga tgggcggcac cttctcaggg
      361 acgacgatca tggagaagaa cctgaccagg ctgacgctga tcgtcgttgg actcttcttg
      421 ttgaccaaca tatccctgta ctttctggga tagcgtctac agatagaagc gctttttgct
      481 gcgtccctgg cattgtcgcc accaagcatc gcttgtggca ggcggcttct gcgatggaag
      541 cacaggcgtc gccggtttga cggagatctc ccgcggaata atcgccgccg gcgaagacaa
      601 aaagaaggac tcgcccgggg aggatgggga gaacaggcga gtccacgcta attatacacc
      661 ccgatgttaa tcagggcgta tatggggtta cagagtcgac cgaccgttca gatccgacgt
      721 agaacgtggt gtcggagggg ggactcgaac ccccaccccc ttatcgggga ctaggccctc
      781 aacctagcgc gtctaccaat tccgccactc cgacagggta gcccgcagag tatacgagag
      841 acatatagta gggtcaacgc cacacactcc attgtgctaa gttctgtgaa tgggtagaaa
      901 gtggttcgtt agggctgtgc tcgctgggtt ggcctgcttc gtcgcgttga ccctgcggaa
      961 gggggggcac cgtaccatcg aaggcggatg gcccgtcaac ctggcccaca ggggcgcgtc
     1021 gtccctggcg ccggagaaca ccctggaggc gttcaggatg gcggtggggg ccggagccgg
     1081 cggcctcgaa ctcgacgtcc acctgacccg ggacggatat gtggtcgtca tccacgacgc
     1141 gaccgttgac cgcacgacga gcgggacggg cgcggtcgcc gagatgaccc tcgatgagct
     1201 tcgcgggctc gatgccggac acggccagta cggcgacagg agcctgcggg tgccaacgct
     1261 cgcagaagtg ctccaagagt tccccggagt cccgatcaac atagacataa agaaggccca
     1321 tcccggagtc gaggaggccg ttctaggagt cttgcgtgac gccggcgccg agggacgtat
     1381 cctcgtagcc tccacgcccc acggtatcat tagccggttc cgcaagctgt caggaggacg
     1441 agtctccacc ggggcgtcca aatgggagat agccgtgttc tacatactga gcaggctcca
     1501 tctggagcgg atcatacgcc cggcctacga cgcgctccag gttccgctgc ggcatagagg
     1561 cataccgctc gtcacgcggc gcttcgtcga agcggcccat gccaggggag tgagggtgga
     1621 cgtgtggacc ataaaccagg caggcgagat gcggcgtctt ctcgacctcg gcgccgacgt
     1681 catcatgaca gatcgccccg agacgctcgc tggcgtgcta cgggagcggt gactcacgcc
     1741 gcgatgagca gcacgatgga ggccacgccg aagagcgtgt aggcggtgcc gagtggaagg
     1801 aagtatctcg ggtcttcccg caacggagac aggtactggg cgtaggggag gatacgggcg
     1861 gcgcgccgga ggacggcgtt gtactcgtag cggaagaaga tcatgtgcag cccgttcgcc
     1921 accgtgacca tgcaaaaagg cacggcccag agcttcgaag ggtacagctt cagagcaggc
     1981 ggcgtaatga agatgcagag cagccagatc acctggatgg tcacgtagct cacccaggcg
     2041 gctgtgatcc tgtatctgtg cctatcgtcg atggtgggat tctaatagag gcgtcgaggc
     2101 gacagcattc tttttgcccg gaatgtaaac tgatctcgac gtaatggatc caccattcgg
     2161 ctcgtagagc ggcatacgga gtaggagaaa catgatccag gaatcactca tcaaagaccc
     2221 ctccctcgcc cctgagggac accgcaagat cgactgggtc gcccagcacg cgccggttct
     2281 gaacaagatc gcaggcgacc aactcgccga cggatctctg cgtggacgca agatcgcgat
     2341 gactatacac ctggaagcga agacggcata tctggcgata ctcctctctg aggccggggc
     2401 tgaggtcacc gtctcgggaa gcaacccgct ctccacccag gacgacgtct gcgcggcact
     2461 cgcggagcgg ggcgtgcggg tcttcgcgac ccacgacccg tccgacgagg actttgaaca
     2521 gtatctgcat cggacgatcg agaccggccc ggacctgatc gtcgacgacg gcgcagagct
     2581 cgtaggacgc ctgatcgaac accaccccga cctcgtcggc aaggttatcg gggcttcgga
     2641 ggagactacg accggcgttt tgaagctgaa agcgatgacg gcggagggcg tactaccgtt
     2701 ccccgtcctg gccgccaacg acgccaggat gaagcacctc ttcgacaacc gctacgggac
     2761 gggtcactca tccatcgtcg ccctcctctc gaacaccaac ctcttcctct cgggcaaggc
     2821 ggtcgttgtg atgggcttcg gctgggtagg gcgcggcctc gccaagtacg ctgacggcat
     2881 gggcgcccgc gtcatagtat gcgagcccga ccctgtgaag ctcttggagg cgtactccga
     2941 gggctacgag gtgatgaact cgctacaggc tgccgaggtg ggggacgtgt tcctgacggg
     3001 cacggggaat ctcaaagtcc tcggcagtga gcacttcgcc aggatgaaag acggcgccct
     3061 tctgacgaac gcgggacact acgatcacga gttcgatctg gccgccctgc gggagactgc
     3121 ggcgcaatcg agagaggttc ggcgcaacat taccgagtat gagctccccg acgggcggcg
     3181 tctgcacgtt ctggcacgtg ggcggctcgt caacatcgcc gcggcggacg gccatccggt
     3241 cgagatcatg gacctcacct tcgccgtgca ggcgctcgcc gcgcatcacc tcgccaacca
     3301 cgccggcgag atagcaccgg gcatccagcc tatccccgag gagatagacg acctcgtcgc
     3361 ccgcgccaag ctggcctctt tgggcgtgga gccggagacg ctgagcgagg agcagatctc
     3421 ctaccagcag agctggcggt agagtaccct agctcctgac gacgag
//
LOCUS       DATAIR010000260         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_385483, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000260 DATAIR010000000
VERSION     DATAIR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385483"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..811
                     /gene="ftsE"
                     /locus_tag="VHM69_06230"
     CDS             125..811
                     /gene="ftsE"
                     /locus_tag="VHM69_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX2740024.1"
                     /translation="MIRFNNVTKVYGNDTVALERVNLEITDGEFVFLVGASGSGKSTM
                     VRLILKEMEPSAGAIYVNGTKLSSVPRRRIPRLRRDIGCVFQDFKLLPNKTAAENVAY
                     AMEVTGQKRRNIRTKVPQILDLVGLSGKMDKYPDMLSGGEQQRVSIARAFVSQPPILV
                     ADEPTGNLDPNTSVGIMQLLHRINRIGTTVVVATHDREMVDVMRKRVVALEDGRIIRD
                     KVRGAYADEV"
     gene            801..1685
                     /locus_tag="VHM69_06235"
     CDS             801..1685
                     /locus_tag="VHM69_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2740025.1"
                     /translation="MRFNFGFFLAEAFKNIKLNLLMSITAVTTTFICILVFGLGLLVS
                     AHIQGVIGSVREDVSVEAFMPDASSEEIDDLANKVEGWPEVASVTQVSEEQAFAEFKD
                     KFKEQPDLYADLDQGVFPASVQIQLNDPGAANDVAERLKQEGFTEDNLSYPQQTIDRL
                     NAVTSYVIWGLYAATLLFLVASVLLISNAIRLSIFARRKEIEVMKLVGASDGFVRTPF
                     VFEGLLQGLVGAGLTAFAVVWINFLFVDWSHNALPYVPISSGAVNAPFVLIVLVTVGV
                     GIGVIGSFLSVTRFLRKI"
     gene            1890..3503
                     /locus_tag="VHM69_06240"
     CDS             1890..3503
                     /locus_tag="VHM69_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX2740026.1"
                     /translation="MILPAMLVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDLVL
                     VNAREKNRSLRGEVIRVIGSAEDPRNVYEALFASIGTSREFPKNVEEEAETVAGREIG
                     ERQDLRYLPTVTIDGADAKDFDDAISVERSGDGYRVWVHIADVTHYVEPGSALDKQAA
                     YRGNSVYLPGTVSPMLPRRLSNDVCSLRPHADRAAVTVEIEVDAEGEAKGYRAFRSVI
                     VSDARLTYEGVDAFLSGEGMVERPDLVQTAYELSRRLKIRAAKRGRLVLGGKEPDYEI
                     DEDGVPVGAGVRRSTPARELIEELMVLANESVAKMLGRKKGGVFRVHERPDEESLELL
                     GQRLAAVGMQVEPTPENLGTISQMAKSDAVNYLILRSIPRAMYSPDDLGHYGLALEHY
                     THFTSPIRRYADVLVHRALFDEEPPDDLADIADYVSEREYRSMICERTSDEYTLMWLM
                     RDRVGEVLEGTVVSTAAFGLFVELETGATGLVHVSKLPGWWELEPNGVVLSNEKIGAS
                     YKVGDRVLVELLDVLPLMQRAELRVVKRL"
     gene            3500..3952
                     /gene="smpB"
                     /locus_tag="VHM69_06245"
     CDS             3500..3952
                     /gene="smpB"
                     /locus_tag="VHM69_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX2740027.1"
                     /translation="MTDFARNKKALHDFSIEETYEAGIQLTGPEVKSVREGRANLKES
                     YARVRDGEVFLIGAHISPYANARQERQLPTRDRKLLLHRKEIDRLIGKSQEEGKTLIP
                     LKLFSKRGIVKLQLAVASRKKQYDKRREIAKKTADREIERAMKERLRR"
BASE COUNT          812 a         1174 c         1260 g          715 t
ORIGIN      
        1 ccggcggttg ataacccggg ccgttgttgc tatcttctcc gatggccagt cgagtccctg
       61 gaggggggac ctcggactaa gctccgtcgt tcgcggcggc cggcaagcca cgcgtggaga
      121 ggtaatgatt cgattcaaca acgtcacaaa agtctacggg aacgacaccg tcgccctcga
      181 gcgggtgaac ctggagataa ccgatggcga gttcgtgttc cttgttgggg cgagcggctc
      241 gggcaagtca accatggtgc gcctgatcct taaggagatg gagccttccg cgggggctat
      301 ctacgtcaac ggaacgaagc tttcatccgt gccgcggcgg aggataccgc gcctcagacg
      361 cgatatcggg tgtgtcttcc aggacttcaa gctgcttcca aacaagacag cggccgagaa
      421 cgtcgcctac gcgatggagg tcacggggca gaagcggcgc aatatccgca cgaaggtgcc
      481 ccagatcctc gacctcgtcg ggctatccgg gaagatggac aagtaccctg acatgctctc
      541 tggcggcgag cagcagcgcg tctccatagc cagagcattc gtctcgcagc cgccaatact
      601 cgtcgcggac gagccgacag gaaacctgga tccgaacacc agcgtgggca tcatgcagct
      661 cctgcacagg atcaaccgca tcggcaccac cgtggtcgtt gcgacgcacg acagggagat
      721 ggttgacgtg atgcgcaagc gggtcgtcgc cctcgaagac gggcgtatta tccgcgacaa
      781 ggtgagggga gcttacgcgg atgaggttta acttcgggtt ctttctggcc gaggctttca
      841 agaacatcaa actgaacctc cttatgagca tcaccgccgt tacgaccacg ttcatctgca
      901 tcctcgtctt cgggctagga cttctggtga gcgcccacat tcaaggggtc ataggctcgg
      961 tgcgcgagga cgttagcgtc gaagcgttca tgcctgacgc gagcagcgag gagatcgacg
     1021 atctcgcgaa caaggtcgaa ggctggcccg aggtcgccag cgtaacccag gtctcagagg
     1081 aacaggcttt cgccgagttc aaggacaagt tcaaggaaca gcccgacctc tacgctgacc
     1141 tcgaccaggg tgtgttcccc gcatcggtcc agatccaact aaacgatccc ggcgccgcca
     1201 acgacgtggc ggagaggctc aagcaagagg ggttcaccga ggacaacctc agctatccgc
     1261 agcagaccat cgaccgcctc aacgcggtca cgtcgtacgt gatctggggc ctctacgcgg
     1321 ccacgctgct cttcctggtc gccagcgtgc tcctgatctc aaacgccata cgactttcca
     1381 tcttcgccag acgtaaggaa atagaagtca tgaagctcgt cggcgcctct gacggcttcg
     1441 tgcgcacgcc tttcgtcttc gaggggctgc tgcagggtct cgtcggcgcc ggcctcaccg
     1501 cgtttgcggt cgtctggatc aacttcctct tcgtcgactg gtcgcacaac gcccttccat
     1561 acgtcccgat atcgagcggc gcagtgaacg cccccttcgt actgatcgtt ctagtaaccg
     1621 tgggagtcgg catcggcgta ataggaagct tcctctcagt aacgaggttt ttgaggaaga
     1681 tctagcgcct gaaggcgcta gatcttcggc ttcaggcaac aggcttcagg tatctgctct
     1741 gtagtggtga tcgctatggc ccgtgctcat gagccggaga cgctggtagc gaacgcccga
     1801 acgcttttgg tgtcaacctg aagcctgtaa cctttagcgt gatagactca tagagtgccc
     1861 tgccagacgt ctctgatcgg agcgctctct tgatattacc ggccatgctg gtccgtcggg
     1921 ggaagtactc ggtagcagac ccgctcttcg tcgaggagcg gcgtccggtg ctggtcgcac
     1981 gcaagttgcg tcgtggggcg gaggccggcg acctggtcct cgtcaacgcc cgcgagaaga
     2041 accgctcgct gaggggcgag gtcatccgcg tcatcgggtc tgctgaggat ccgcgcaatg
     2101 tctacgaggc gctcttcgcc tctattggga cctcccgcga gtttccgaag aatgtcgaag
     2161 aggaggcaga gacggtcgcg gggcgggaga tcggggagcg ccaggacctc aggtatctcc
     2221 ctactgtgac aatagacgga gccgacgcga aggacttcga cgatgcgatc tcggtcgagc
     2281 gttccggaga cggatataga gtctgggtcc acattgccga cgtgacccac tacgtggagc
     2341 caggctcagc gctggacaaa caggccgcct acaggggaaa ctcggtttac cttccaggaa
     2401 ccgtgtcgcc gatgctcccc aggcgtctct cgaacgacgt gtgctccttg aggccgcacg
     2461 ccgacagggc tgccgtaacg gtggagatag aagtcgacgc cgagggcgag gcgaagggct
     2521 accgggcttt ccggagcgtc atcgtcagcg acgccaggct cacgtacgag ggcgttgacg
     2581 cgttcctgag cggtgagggg atggtggaga ggccggatct ggtacagacc gcttacgaac
     2641 tgtcgcgcag gctgaagata agggccgcga agcggggcag gctggtactc ggcgggaagg
     2701 agccggatta cgagatagac gaagacgggg tgcccgtggg tgcgggagtc cgccggtcca
     2761 ctccggccag agagttgatc gaggagctaa tggtcctcgc gaacgagtcg gtcgcgaaga
     2821 tgctgggacg taagaagggc ggcgtcttca gggtgcacga gagacccgat gaggaatccc
     2881 tggaactgct gggacagagg ctcgccgccg tcgggatgca ggtcgagcca acgccggaga
     2941 accttggcac catctcccag atggccaaat ccgacgctgt gaactacctg atcctgcgtt
     3001 ccatccctcg cgctatgtat tctcccgatg accttggcca ttacgggctg gcgctcgagc
     3061 actacacgca cttcacgtcc ccgatccggc gctacgcaga cgtcctcgtc caccgcgccc
     3121 tcttcgatga ggagcccccc gatgacctcg ccgacatcgc cgactacgtc tcagagcggg
     3181 agtacaggtc catgatctgc gagcgcacct cagacgagta cacgctgatg tggctgatgc
     3241 gcgaccgggt cggggaggtt ctcgaaggaa ccgtcgtgag cactgccgcc ttcggcctct
     3301 tcgtcgagct cgagacgggc gccacgggcc tcgtccacgt gagcaagctc cctgggtggt
     3361 gggagctcga gccgaacggt gtcgtacttt ccaacgagaa gatcggagcc tcgtacaaag
     3421 tgggggacag ggtcctggta gagttgctcg acgttctccc gctgatgcag cgggccgagc
     3481 tcagggtggt gaagcgcctg tgacggactt cgcgcgcaac aagaaagccc tgcacgactt
     3541 cagtatcgag gagacgtacg aggccggcat ccagctcacc ggccccgagg taaagtccgt
     3601 cagggagggt agagcaaacc tcaaagagag ctatgcgcgg gttcgggacg gcgaagtgtt
     3661 cttgatcggg gcgcacatct ctccgtatgc gaacgccagg caggaacgcc agctccctac
     3721 ccgcgaccgc aagctgctct tgcacagaaa agagatcgac cgtctcatcg gcaagagcca
     3781 ggaggaggga aagaccctca tcccgctgaa gctcttctct aagagaggca tcgtcaagct
     3841 ccagctcgca gtggcaagcc gcaagaagca gtacgacaag cggcgtgaga tcgccaagaa
     3901 gaccgcggac cgcgagatcg aacgcgccat gaaagagcgc ctccgccgct agagcgaatt
     3961 g
//
LOCUS       DATAIR010000261        11873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_108805, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000261 DATAIR010000000
VERSION     DATAIR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108805"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..4283
                     /locus_tag="VHM69_06250"
     CDS             348..4283
                     /locus_tag="VHM69_06250"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2740028.1"
                     /translation="MAGRARPLRALADGVWAVSALAARRHLASDSGDLAGATVQISSG
                     FVDGQDDLLFDDQNGITGSVSGDTLTLSGSSSVANYQTALRSIRYDNASDTPDTNQRT
                     VSFQVDDGSSVSNLSNTATKDVSVAAANDAPEIDLNGAGTGYSATFTEDGPAVSIVDS
                     ANLTVTDADDTNIESATVTITNLQDDAESLDADVGATGITKSYDSSTGVLTLGNSATK
                     AQYRQVLRTVTYDNSSQNPGTTGRNVTFVVNDGEDDSNTATSDVAVNAVNDAPELDQP
                     DDKLTYTEDTPSENHALAVAPNLTVDDVDDANLQSGTVQITTNLQSPQDELLFTNQNG
                     ISATGSGTGSLTLSGSATKANWRNALRSVQYRNTSDAPDTSQRTVSFRVSDGHTGQSP
                     DSNIVTRDIEVVATNDAPVADNESFSGTDEAVGNTSLVVNDLTDGAPDPTGPQKTVTG
                     DILDGDTDPDNAPAELTVKVETKTTADGGSITFQADGDFTYHPPAGCDGGSSDTVQYT
                     VEDNHPTDEKTGNGTLTINISQCVWYVDDSAPAGGDGRSHLPYNSLTPLNNSATDADV
                     AGERIFLYDGTYSGGLALENNQPLFTEKHGLVVGDGGAGNLTLEPNDGTGSTLNGGLA
                     AASGNNVQGLSLGTTGSSSVYALSGTSVGTFTMNNVTGGDLNNPAGGAINIGGTGNAL
                     NLQFGTLSSSGGSGNAVSILAASGTVSGQSGTLSNATGTTVNLNGGSLDFTLNGAVSD
                     DAGQLISISNKTSGTTDFNGPVDDVTTTANNGGGISLQSNGTALTRFDGGVNLSTGSA
                     NAISASNGGTLAIPDPAGAATNSIVSTTGTPLNIASTTIHGDDLVFEKISSNGSTNGI
                     VLNNTGTSGNLSVTGNGGVCTIATPTCSGGTIQSTTGEGISLTNTSSPSFNLMRVLNT
                     AGHGISGTTTSGLSLSNSLVQGAGDADNEAGLFFATVNGTNLTGNSSITNTVVHAPAD
                     VGAFIQNYGGNLNLTVSGSTFSGTQKNTPGVVTGDDLLRMIADRTNAGSATITARIIG
                     STFRNSESDDILANAQSSTGSGGGTMNLTVDGNTFDGNGVFETCDTIGCNSFGIVTQG
                     TQGATIRYTIQNNNIVNHLDSAIAARSDNSSNVQGFIKNNTIGTAGSPRSGSYRDRGI
                     FLASDDSSDFIASVENNVIHSTFFEGISASTTDGTGLSPMMDLTILGNTTPTPADTSG
                     TVFGSIGMTASGNASACARAENNTAQPGGGGGGSIDFLLFRDTATSTATFKLEGLPAS
                     TADASAFVKSKNPASADPVFAIAGKDGSNPSTPQFTVGTCRTPAATPTP"
     gene            4673..5173
                     /locus_tag="VHM69_06255"
     CDS             4673..5173
                     /locus_tag="VHM69_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX2740029.1"
                     /translation="MSEPFIGETRWISWNYAPRGWAFCNGQLLPINQNQALFSLLGTM
                     YGGDGRVNFALPDLRGRAPVHVGGGFTQGQRGGEEAHALTGTEMPQHIHGVTASGGSG
                     TGAAPAQSFLANRQSNIYVPPANLQPMNPGAVSQTGGSQPHPNMAPYLVLNCVIALIG
                     IFPSRN"
     gene            5186..5701
                     /locus_tag="VHM69_06260"
     CDS             5186..5701
                     /locus_tag="VHM69_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX2740030.1"
                     /translation="MSQPFIGEIRTVGFSFAPVGWAFCNGQTIPISENEALFNLIGTT
                     YGGDGQETFALPNLQSRFPIHAGQGPGLTNHTLGEVGGTESVTLTVQQMPTHTHQLLG
                     SGNSATEETPQANVTGQPVTNIYGNLPPFQEMDPNVVGPAGGSQPHENVHSFQVINFI
                     IALEGIYPPQS"
     gene            5716..6234
                     /locus_tag="VHM69_06265"
     CDS             5716..6234
                     /locus_tag="VHM69_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX2740031.1"
                     /translation="MADPFVGEIRTFGFNFPPTGWAQCNGQLLPISQNTALFALLGTY
                     YGGDGKSTFALPNLQGASPMDQGQGPGLSDRYIGELGGAGSVTLIQSEMPSHTHVVSG
                     SGRPANENDANNFYWGAGVANMYNAAANPPQQMAPQTLGPAGGSTPHNNMQPYLVLNF
                     CIALQGIFPARG"
     gene            6236..6727
                     /locus_tag="VHM69_06270"
     CDS             6236..6727
                     /locus_tag="VHM69_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740032.1"
                     /translation="MESPITYDLKLRPVEPGDEAFLYRVYASTREEELARTGWDETQK
                     AAFLRMQFDAQSSYYEEHYRGAEFSVILSGGHPAGRLYVARLREEIRIVDIALLPGHR
                     GMGIGTRLLNDLISESEASGQFLSIHVERFNPALRLYERLGFRAVEDKGVYLLMERPP
                     RSR"
     gene            complement(6720..7025)
                     /locus_tag="VHM69_06275"
     CDS             complement(6720..7025)
                     /locus_tag="VHM69_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740033.1"
                     /translation="MLENFTIETFSEHLGSTFNIHPDDTGQVEVELISATGLGGSAGE
                     EPPDRKRPFSIIFRGPGDILLPQRIYRMEHEEIGSFELFLVPIGPDEKGLRYEAIFT"
     gene            complement(7072..7785)
                     /locus_tag="VHM69_06280"
     CDS             complement(7072..7785)
                     /locus_tag="VHM69_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740034.1"
                     /translation="MNSRVAAVLGVGPGLGAAVAGRFAREGFDVALMARTEESVAPVR
                     EEIVATEGRALSVSADATDPDSVAAAFEKVRGELGDPEVFVYNAGAFQMGGILELSPG
                     QFDECFKANCAGAFYAAQQVLPAMVEAERGTVLLTGATASLRGSARFSALAVGKFGLR
                     ALAQSMAREFGPQGIHVAHVIIDGQINTPRMREMSPDREDHTMLSPESIAETYWQLHS
                     QDRTAWTLELDLRPAVESF"
     gene            complement(7782..9092)
                     /locus_tag="VHM69_06285"
     CDS             complement(7782..9092)
                     /locus_tag="VHM69_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HEX2740035.1"
                     /translation="MTTITGVVARDIRFPTSEDLAGSDAMNQAPDYSAAYVTIKTDTD
                     LEGHGLTFTIGRGNELCVAAIDALAPLVAGRSLETFKRDMGAFWRHVTGDSQLRWVGP
                     EKGVIHLATAAVVNAVWDLYAKSEGKPLWKLLSDMSPEELVRCVDFRYITDAITPDGA
                     LEILRENEETKQAREAEMLSDGFPAYTTSAGWLGYPDEKVRRLSREAVEAGWGHVKIK
                     VGLDVEDDARRAAIVREEIGPERKLMLDANQVWDVDEAIENMEILRQFDPYWIEEPTS
                     PDDVLGHAAIAKAVDPIGVATGEHCQNRILFKQLFQANAISFCQIDSCRLGGVNEVIA
                     VLLMAKKFGVPVCPHAGGVGLCEYVQHLSIFDYICIGASLEDRILEYVDHLHEHFLDP
                     VKIKDGRYMPPTVPGYSIEMKPESLDAYEFPKGSAWTEQTRSGV"
     gene            complement(9089..9742)
                     /locus_tag="VHM69_06290"
     CDS             complement(9089..9742)
                     /locus_tag="VHM69_06290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740036.1"
                     /translation="MSEAHRRTVESGYDYMAKQYLATKDPKDPLALAALEDLASVVPQ
                     GAAVLDLGCGAGVPVTRWLADRGFAVTGVDVSARQLDLARSNVPTATFIKADMTALAF
                     VPHTFDAAVAFHSIIHVPRTEQPALLARIHRWLKPGGVFLATMTVTEYEGREEDWEGW
                     GAPMAWSHYDRDANVAMLREARFEIQYAEPRTGKGTGDEDETWLWVLARKSSREGAT"
     gene            complement(9739..10494)
                     /locus_tag="VHM69_06295"
     CDS             complement(9739..10494)
                     /locus_tag="VHM69_06295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2740037.1"
                     /translation="MERLRGKRAIVTGGGSGIGRAIALRFSQEGARVVLSDVDEEAAR
                     VVGAVAGNETLVHKTDVTRSDEVEALVQTAVEAWGGLDIMVNNAGIGVAGTATNTSEE
                     DYERVMDVCLRGTFLGMKYAIPAIRDSGGGSVINMSSVAALVGITDRAVYSAAKGAIL
                     AITRAAAIDHVSEGVRVNSIAPGTVDTPWIGRITAGYDDPEEARARMQARQPHGRFVT
                     PEEIAAMAAYLASDESASVIGACMIVDGGVTAR"
     gene            10582..10911
                     /locus_tag="VHM69_06300"
     CDS             10582..10911
                     /locus_tag="VHM69_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740038.1"
                     /translation="MNPYAGWIQDLPQVDTPFEGLDGRMISGPDGQAVFFRAAERFEV
                     PPHAHGAQWGIVVTGLLRLTIEGEPATYEPGETYDIPAGAEHAAILEAGTCVIDVFQD
                     PDRYSPK"
     gene            10911..11627
                     /locus_tag="VHM69_06305"
     CDS             10911..11627
                     /locus_tag="VHM69_06305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX2740039.1"
                     /translation="MRSPFDGYEWVDLSHTLEEDIPAYPTHARFGKTLYESYEFGDVA
                     RHYGLTMSEHTGTHMDAPLHFISEGPSHYGTDEIPLDRLAGRAATIDATELGVGDLLA
                     VEHVEGWEEEHGPIEAGDRVLIRYGWDERWTTGPEGRRFLEDWPGLSGGAAEYLVGKG
                     VSLVGCDTLAVDATVSESNPAHYALLGNEVYIVENLKNLDLLPPFCLFIALPLKIKGG
                     SGSPVRAVALIGRAPRPHVQ"
     gene            11661..>11873
                     /locus_tag="VHM69_06310"
     CDS             11661..>11873
                     /locus_tag="VHM69_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2740040.1"
                     /translation="MKGIVWHGPEQMSVEEVPEPEIEPGAVIVRPTATGICGSEIEGY
                     LGRMGNRTPPLVMGHEFAGTVTEVGEG"
BASE COUNT         2263 a         3909 c         3727 g         1974 t
ORIGIN      
        1 cccgttcggc cagggcggcc cggatcgagc ctgagcggct ccactggcca agggtgcgca
       61 cgtcctcggg gggtatggcg agtgggggtg cgatggcagg catgaacaat cctgccgcca
      121 ccactcctca accgtcaagt aagttcagag acgacctact agggtgtgtt tcaagagtgg
      181 ggggtcccgt gcgatgctcc cgccatggga cgaggagacc tgaccaacga gcaatgggct
      241 aggctggcgc cgctgctgcc caccggtaag aagcacggcc ggccacccgc ctggggcaag
      301 cggcaactca ccaacggaat ccgctggcgg acccggaccg gggcgccgtg gcgggacgtg
      361 cccgcccgct acgggccctg gcagacggtg tatgggctgt ttcggcgctg gcagcgcgac
      421 ggcacctggc ctccgactcc ggcgacctcg ccggggcgac ggtacaaatt tcgtcaggct
      481 tcgtcgacgg ccaggatgac ctcctgttcg acgaccagaa cgggatcacg ggcagcgtaa
      541 gcggcgacac tctgacgctc agcggctcat ccagcgtggc caactaccag acggctctgc
      601 ggagcatccg ctacgacaac gcgagcgaca cgcctgacac gaatcagcgc acggtctcgt
      661 tccaggtcga cgacggctct tcggtgagca acctctccaa caccgcgacg aaggacgtct
      721 ctgtcgcggc ggcgaacgat gcacccgaga tcgacctcaa cggcgcgggc accgggtact
      781 cggccacgtt caccgaggac ggcccggcgg tctccatcgt ggatagcgcc aacctcaccg
      841 tcaccgacgc cgacgatacg aacatagagt cggccaccgt gaccataacc aacctccagg
      901 acgacgccga gtccctcgac gccgacgtgg gcgccaccgg catcaccaag agctacgact
      961 cctcgaccgg cgtccttacg ctcggcaact ccgccacgaa ggcccagtac cggcaggtgc
     1021 tgcggacggt cacgtacgac aattcttcac agaaccccgg caccaccgga cgtaacgtta
     1081 ccttcgtggt caacgacggc gaggacgact ccaataccgc gacgagcgac gtcgccgtca
     1141 acgcggtcaa cgacgcgccg gagctcgacc agcccgacga caagctgacc tacaccgagg
     1201 acaccccgtc cgagaaccac gctttggccg tcgcgccgaa cctgacggtg gacgacgtcg
     1261 acgacgccaa cctgcagtca ggcacggtcc agatcaccac caacctgcag agcccgcagg
     1321 acgagctgct gttcaccaac cagaacggca tctccgccac gggaagcggc accgggtcgc
     1381 tcaccctgag cggcagcgcc acgaaggcca actggcggaa cgcactgcgc agcgtccagt
     1441 accgcaacac cagcgacgct cccgacacgt cgcagcgcac ggtctcgttc cgggtgagcg
     1501 acggccacac cggccagagc cctgacagca acatcgtcac gcgcgacatc gaggtcgtgg
     1561 ccaccaacga cgcgccggtc gcggacaatg agtcgttctc cggtaccgat gaagccgtcg
     1621 gcaacacctc gctcgtggtc aacgatctca ccgacggcgc gcccgacccg accgggccgc
     1681 agaagacagt cacaggcgac atcctcgacg gcgacaccga cccagacaac gctcccgccg
     1741 agctcaccgt caaggtggag accaagacga ccgcggacgg cggatccatc accttccagg
     1801 cggacggcga cttcacgtac cacccgccgg ccggttgtga cggcggctcc tcggacacgg
     1861 tccagtacac ggtcgaggac aaccacccga ccgacgagaa gaccggcaac ggaacgctta
     1921 cgatcaacat ctcccagtgc gtctggtacg ttgatgactc cgcgcctgcc ggcggcgacg
     1981 ggcgctcgca cttgccgtac aactcgctca cgccgctgaa caacagcgcc accgacgccg
     2041 atgtcgcggg agagcggatc ttcctctacg acggcaccta ctccggtgga ctcgcgctcg
     2101 agaacaacca gccgctgttc accgagaagc acggcctcgt cgtcggcgac ggcggcgcgg
     2161 gcaacctcac tctggagccg aacgacggca ccggcagcac gctgaacggc ggcctcgcgg
     2221 cggcgtccgg caacaacgta caggggctga gcctcggcac caccggctcc tcgtcggtgt
     2281 acgccttgtc gggaacctcg gtcggcacct tcaccatgaa caacgtcacc ggcggcgacc
     2341 tcaataatcc ggccggaggt gcgatcaaca tcggcggcac cggcaacgcg ctgaacctcc
     2401 agttcggcac gctctcctcg agcggcggct caggcaacgc ggtgtcgatc ctcgcggcct
     2461 caggcaccgt gagcgggcag agcggcacgc tctcgaacgc gaccggcacg accgtcaacc
     2521 tcaacggcgg ctcgcttgac ttcactctga acggcgcggt cagcgacgac gcaggccagc
     2581 tcatctcgat ctcgaacaag acgtcgggca cgacggactt caacggcccg gtcgacgacg
     2641 tcaccaccac cgccaacaac ggcggcggga tctcgctcca gtccaacggc accgcgctca
     2701 cgcgcttcga cggcggggtg aacctgtcca ccggcagcgc gaacgccatc tccgcgtcca
     2761 acggcggcac gctcgcgatc cccgacccgg ctggcgcggc gaccaactcg atcgtgtcca
     2821 cgacgggcac gcctctgaac atcgcgtcca cgactatcca cggcgacgat ctcgtgttcg
     2881 agaagatcag ctcgaacggc tccaccaacg gcatcgtgct caacaacacc ggcacgagcg
     2941 gcaacctgtc ggtgacgggc aacggcgggg tctgcaccat cgccactccg acgtgctcgg
     3001 gcggcacgat ccagagcacc accggcgaag gcataagcct gacaaacacg agcagcccgt
     3061 cgttcaacct catgcgcgtc ctgaacaccg cggggcatgg gatctcgggc accaccacct
     3121 cggggctgtc gctgtcgaac tcgctggttc agggagcggg cgacgcggat aacgaggcgg
     3181 ggctcttctt cgccaccgtg aacgggacga acctcacggg taacagctcg atcacgaaca
     3241 ccgtggtcca cgcgcctgcc gacgtcggtg cctttatcca gaactacgga ggtaacctga
     3301 acctgacggt ttcgggctcg accttctccg gtacgcagaa gaacacgccg ggcgtggtaa
     3361 caggggacga cctgctgcgc atgatcgccg accggacgaa cgcgggctcg gccacgatca
     3421 ccgccagaat aatcggcagc accttcagga actcggagag cgacgacatc ctcgccaacg
     3481 cccagagcag caccggctcg ggtggcggca cgatgaacct gaccgtggat ggaaacacct
     3541 tcgacggcaa cggcgtcttc gagacgtgcg acaccatagg ctgtaactcc ttcggcatcg
     3601 tcacgcaggg tacccaggga gctacgatac ggtacacgat ccagaacaac aacatagtca
     3661 accacctcga ctcagcgatc gcggcgcgct ctgacaacag ctcgaacgtg cagggcttca
     3721 tcaagaacaa cacgatcggc acggcggggt ccccgcgctc tggttcgtac cgcgaccggg
     3781 gtatcttcct ggccagcgac gacagctccg actttatagc gagcgtggag aacaacgtca
     3841 ttcactccac cttcttcgag ggtatctccg cttccacgac cgacggtacg gggctctccc
     3901 ccatgatgga cttgacgatc cttggcaata caacaccgac gccggcggac acgtcaggca
     3961 ccgtctttgg ctccatcggc atgactgcta gcggtaatgc cagtgcctgc gccagggcgg
     4021 agaacaacac agcccagccg ggcggtggcg gtggaggctc catcgacttc ctcctgttcc
     4081 gcgatacggc tacgagtacg gcgaccttca agctcgaggg tcttcctgcg tcaacggccg
     4141 atgcgagcgc cttcgtgaag agcaagaacc ccgcctcggc ggatcccgtc ttcgccatcg
     4201 cgggcaagga tgggagcaac ccgagcacgc cccaattcac cgtcggcacc tgccgcaccc
     4261 cggcggccac gccgacgccc tgagagccgg cagcgcggga gggacgaacc cctcccgcgc
     4321 tgagacgatc caaagggtga ctacgtcatc actgtagaga acgggcaatc gggcataagg
     4381 ctacgactga gatggtcagg tggcgggatg taggaacacc gtttcgccag gcgtgagcat
     4441 acgcagcgcg tttcctcaca aggagtggaa gcttgagtct ttcgcggcta cttgccgcta
     4501 tggcggcgat tttcttaagt atttgccaca agattcattg gggattcatc cctatgtgtt
     4561 ctcatgaagc tgctttaaga cgagagtaag gaggaaaggc agccagagcc cagtcaaagt
     4621 tgtccaatac attgatggag caatcgtcat ccatcgacag aaaggagcca cgatgtccga
     4681 gccgtttata ggagagacga ggtggatcag ctggaactac gcgccgaggg gttgggcttt
     4741 ctgcaacggc cagctactcc cgatcaacca gaaccaggcg ctgttttcgc tgctgggaac
     4801 catgtacggc ggcgacggac gcgtgaactt cgcgctgcct gacctgcgcg ggcgcgcgcc
     4861 ggtgcacgtc gggggcgggt tcacccaggg gcagaggggc ggcgaggagg cgcatgcgct
     4921 gaccggtact gagatgcctc agcacataca cggtgtcacg gcgagcgggg gaagtgggac
     4981 tggggcggcg cccgctcaga gcttcctggc gaacaggcag tcgaacatct acgtgccgcc
     5041 ggcgaacctg caaccgatga acccgggcgc ggtgagccag acgggcggca gccagccgca
     5101 cccgaacatg gcgccctact tagttctcaa ctgcgtcatc gccctgattg gcattttccc
     5161 gtcgaggaac tgagaggagg caaggatgtc gcaaccattc ataggcgaga tccggacggt
     5221 gggcttcagc ttcgccccag tgggctgggc tttctgcaac ggacagacta ttcccatctc
     5281 tgagaacgag gcgctcttca acctgatcgg gacgacgtac gggggtgacg gacaggagac
     5341 gttcgcgctg cccaacctgc agagccgctt ccccatccac gcgggacagg ggccggggtt
     5401 gacgaaccac acgctgggcg aggtaggcgg cacggaaagc gtgacgctga ccgtgcagca
     5461 gatgcccacg cacacccacc agctgctcgg ctcggggaac tcggccacgg aggagacacc
     5521 gcaagcgaac gtgacggggc aacccgtgac caacatctac gggaacctgc caccgtttca
     5581 ggagatggac ccgaatgttg tgggaccggc cgggggcagc cagccgcacg aaaacgtcca
     5641 ttcgttccag gttatcaact tcatcatcgc gttggaaggc atctatcctc cgcagtccta
     5701 gaaaggaggc acaccatggc agatccattc gtcggcgaga tccggacctt cggcttcaac
     5761 ttccctccta ccggctgggc gcagtgcaac gggcagttgc tcccgatctc gcagaacacg
     5821 gcgctgttcg cgctgctcgg cacgtactac ggtggcgacg gcaagtcgac cttcgcgttg
     5881 cccaacctgc aaggagcctc acccatggac cagggacagg gaccggggtt gtcggatcgg
     5941 tatatcggtg agctgggcgg ggcgggttcc gtcacgctaa tacagtcgga gatgccgtca
     6001 cacacgcacg tggtcagcgg gtcagggcgg ccggcgaacg agaacgatgc gaacaacttc
     6061 tactgggggg cgggggtggc gaacatgtac aacgctgccg caaacccgcc gcagcagatg
     6121 gcgccccaga cgcttggacc cgccggcggc agcacgccgc acaacaacat gcaaccttac
     6181 ctggtgctca acttctgcat cgcgctgcag ggaatcttcc ccgcgcgcgg ttgacgtgga
     6241 gagcccgatc acgtacgacc tcaagctgcg gcccgtcgag ccgggcgacg aggcgttcct
     6301 ctaccgggtg tacgcgagca cgcgggagga ggaactggcc cggacgggct gggacgaaac
     6361 gcagaaagca gccttcctgc ggatgcagtt cgacgcccag agcagctatt acgaggagca
     6421 ctatcggggg gctgagttct ccgtgatcct gtccggcggt catcccgccg gcaggctcta
     6481 cgtcgcccgg ttgcgggagg agatccggat cgtggacatc gccctcctgc ccgggcacag
     6541 gggcatggga atcgggacgc gcctgttgaa cgatctcatc tccgagtcag aagcgtcggg
     6601 ccagttcctc tctatacacg tcgagcgatt caatccggcg ctgcgcctct acgagaggct
     6661 cggctttcgt gcggtggaag acaagggcgt ctacctgctc atggagcggc cgccacgctc
     6721 taggtaaaga tcgcctcgta gcggagccct ttctcgtccg ggccgatggg gacgaggaac
     6781 aactcgaagc tccctatctc ctcgtgctcc atgcggtaga tgcgctgggg gagcaggatg
     6841 tctcctggac cgcggaatat tattgagaag ggccgcttgc gatctggcgg ctcttcgccg
     6901 gcgcttccgc ccaacccggt ggccgagatc agctcgactt ccacctgtcc cgtgtcgtca
     6961 ggatgaatat tgaacgtgct gccaaggtgt tcggagaagg tctcgatggt gaagttctcc
     7021 agcatgtaca gatcctctct tcggaagcct ctcagccctg aatgtcctct atcaaaagct
     7081 ctctacggcg ggacgcaggt ccagctccag cgtccaggcg gtcctgtcct gcgagtgtaa
     7141 ctgccagtag gtttccgcta tagactctgg cgagagcatg gtgtggtcct cacggtcggg
     7201 ggacatctcg cgcatcctgg gagtgttgat ctggccgtct atgatgacgt gcgcgacgtg
     7261 tatgccttgt ggaccgaact cccgcgccat cgactgcgcc agcgcacgca aaccaaactt
     7321 gcccacggcg agtgcagaga accgcgccga gcctcgcaaa gatgccgtcg cgccggtaag
     7381 aagtacggtg cccctctctg cctcgaccat cgcgggcagt acctgctgcg ccgcgtagaa
     7441 ggctcccgcg cagttggcct tgaagcactc gtcgaactgc cctggcgaca gctccaggat
     7501 gccacccatc tggaaagcac ccgcgttgta gacgaagacc tcggggtcgc ccagttcgcc
     7561 acgcaccttc tcgaaggcgg cggcgacaga gtcggggtcc gtggcgtcgg cggagacgga
     7621 cagcgccctg ccttcggtgg ctactatctc ttccctgaca ggcgcgacgc tctcctccgt
     7681 gcgcgccatg agggcgacgt caaagccctc gcgggcgaag cgcccggcca ccgccgcgcc
     7741 gagcccgggt ccgacaccga gaaccgccgc taccctgctg ttcatactcc gctccttgtc
     7801 tgttcggtcc acgcgctccc tttcgggaac tcgtatgcgt ccagtgactc gggcttcatc
     7861 tcgatgctgt agccaggcac ggtcgggggc atgtagcggc cgtctttgat cttcaccggg
     7921 tccaaaaagt gctcgtggag atggtcgacg tactccagga ttcgatcttc gagcgaggcg
     7981 ccgatacaga tgtagtcgaa gatcgagagg tgctgtacgt actcgcacag cccgactccg
     8041 ccggcgtgcg gacagacggg gacgccgaac ttcttcgcca tcagcaacac ggcgatgacc
     8101 tcgttgacgc caccgaggcg gcatgagtct atctggcaga aagagatagc attcgcctgg
     8161 aagagttgtt tgaagaggat gcggttctgg cagtgctcgc ccgtggcgac ccctataggg
     8221 tcaaccgcct tcgctatcgc ggcgtggccg agcacgtcgt ccgggcttgt gggctcctcg
     8281 atccagtacg ggtcgaactg cctcaggatc tccatgttct ctatcgcttc gtccacgtcc
     8341 cagacctggt tcgcatccag catcagcttg cgttctgggc ctatctcctc cctgacgatg
     8401 gcggcacgtc tggcgtcgtc ctccacgtcc agacccactt tgatcttgac gtgcccccag
     8461 ccagcctcca ccgcctcccg cgagagtcgc ctgaccttct cgtccggata gccgagccag
     8521 cccgcgctcg ttgtgtaagc ggggaaaccg tccgagagca tctcagcttc ccgagcctgc
     8581 ttcgtctctt cgttttcacg caggatctcc agcgccccat ccggtgtgat ggcgtctgtt
     8641 atgtagcgaa agtcaacgca gcggaccagt tcctcagggc tcatgtccga gaggagcttc
     8701 cagagcggtt tgccctcgct ctttgcgtag aggtcccaga cggcgtttac gaccgcggcg
     8761 gtggcgaggt ggatcacacc cttctcaggc ccgacccacc tgagctggct gtctccggtc
     8821 acgtgccgcc agaaggcgcc catgtcccgc ttgaacgttt cgaggcttct tccggcgacg
     8881 agcggggcga gcgcatctat tgcggcgacg cacagctcgt tgccgcgccc gatggtgaag
     8941 gtgagaccgt ggccttcgag gtcggtgtcc gtcttgatgg tcacgtacgc cgcagagtag
     9001 tctggggcct ggttcattgc gtccgagccg gcaaggtcct cggaggtggg aaagcggatg
     9061 tcgcgggcta ctaccccggt gatggtggtc acgtagcacc ttccctcgaa ctcttgcggg
     9121 caaggaccca gagccaggtc tcgtcctcgt cgccggtgcc cttgccggtg cgcggctcgg
     9181 cgtattggat ctcgaatcta gcctcgcgta gcatcgccac gttggcgtct ctgtcgtagt
     9241 ggctccatgc catcggcgcc ccccagcctt cccagtcctc ttcccggcct tcatattcgg
     9301 tgacggtcat ggtcgccaga aagactccac ctggcttcag ccagcggtgg atgcgcgcaa
     9361 ggagcgctgg ctgctcggtg cgtggcacgt ggatgatgga gtggaaggcg accgcggcgt
     9421 cgaaggtatg gggtacgaag gccagtgcgg tcatatccgc cttgatgaag gtggccgtgg
     9481 gcacgttgct cctggcaaga tccagttgcc tggccgagac gtccacgccg gtgacggcga
     9541 accctctgtc ggcgagccag cgcgtgacgg gcacgccggc gccgcaaccc agatcgagca
     9601 cggcggctcc ctgcggcacg acagaggcca ggtcctcaag agccgcgagg gcgagcgggt
     9661 ctttggggtc tttggtggcg aggtactgtt tggccatgta gtcgtagcca ctttcgacgg
     9721 tgcgcctgtg ggcttcgctc acctggctgt cacgcccccg tccacgatca tgcaggctcc
     9781 gatcaccgag gccgactcgt cggaggcgag gtaggccgcc atcgccgcga tctcctcagg
     9841 cgtgacgaac cggccgtgtg gctggcgggc ctgcatcctc gcgcgcgctt cctcggggtc
     9901 atcgtatcct gcggtgatgc gcccgatcca gggcgtatcc accgtaccag gcgcgatgct
     9961 gttgaccctc acgccttctg agacgtgatc tatggcggcg gcgcgggtga tggcgaggat
    10021 cgcgcccttt gccgcgctgt agacggccct gtcagtgatc ccgacgagag cggcaacgga
    10081 cgacatgttg atgacagacc caccaccaga atctcttatg gcggggatcg cgtacttcat
    10141 ccccaggaac gtgcctcgca ggcacacgtc catcaccctc tcgtagtcct cctccgaggt
    10201 gttggtggca gtccccgcga cgcctatccc cgcgttattg accataatgt cgaggccgcc
    10261 ccacgcttcg accgccgttt gcacgagcgc ctcgacctcg tccgagcggg tgacgtcggt
    10321 tttgtggacg agggtttcgt tgccggctac tgcgccaacg actctagccg cttcctcgtc
    10381 cacgtccgag aggactacgc gggccccctc ctgcgagaac ctgagggcga tggcgcggcc
    10441 tatgccggaa ccaccgcccg tgactatggc cctcttccct ctgaggcgtt ccatctcacc
    10501 cccggatgta gtaggaacga ccagtataat gacgccctga ccggcggcgc aaacgcgccg
    10561 tccgcgagga aaggagacgc aatgaaccca tatgccggtt ggatccagga cctaccccag
    10621 gtggatacgc ctttcgaggg tctggacgga cgtatgatct caggcccgga cgggcaggcc
    10681 gttttcttcc gggccgcgga gaggttcgag gtcccgccac acgcacacgg cgcccagtgg
    10741 ggaatagtag ttactggtct actgcgcctg accatagaag gcgagccggc cacctacgag
    10801 cctggcgaga cctacgacat acccgccggg gcagagcatg ccgccatact cgaagccgga
    10861 acctgcgtca tagacgtctt ccaggacccc gaccgctaca gcccgaagta atgaggtcgc
    10921 cgttcgacgg ctacgagtgg gtggacctct cccatacgct ggaggaagac attcccgcct
    10981 acccgacgca cgcccgcttc ggcaaaaccc tctacgagtc ctatgaattt ggtgacgtcg
    11041 cacgtcacta tggcctcacc atgagcgagc atacagggac ccacatggac gcgcccctcc
    11101 acttcatatc tgaggggcct tcccactacg ggacggatga aatccccctg gaccggctcg
    11161 ccggacgcgc cgcgaccatc gacgctacgg aactcggggt gggcgacctg ctggccgtag
    11221 agcacgtcga gggttgggag gaggagcacg gtcccatcga ggcgggggac agagtcttga
    11281 tccgctacgg ctgggacgaa cgatggacga cgggacccga gggacggcgc tttctcgaag
    11341 actggccggg cctctcaggc ggggcggccg agtacctggt cgggaaggga gtgtctctcg
    11401 tcggctgcga tacgctagcc gtagacgcca ccgtttcaga gtcgaacccg gcccactacg
    11461 cgctgctcgg caacgaggtg tacatcgtgg agaacctgaa gaacctggat ttgctaccgc
    11521 cgttttgcct ctttatcgcg ttgccgctaa agatcaaagg tgggtcggga tcgccggtcc
    11581 gggctgtcgc gctgatcgga cgcgcgccga ggccgcacgt acaatagagt ggggaagttc
    11641 gctacgggaa ggtggaaaac atgaaaggga tagtgtggca cgggcctgag cagatgtccg
    11701 tcgaggaggt gccagagcct gagatcgagc ctggggccgt gatcgtccgc ccaacggcga
    11761 cggggatctg cggctctgag atcgaaggct acctcggaag gatgggcaac cgtaccccgc
    11821 ccctggtgat gggccacgag ttcgcaggca ccgtcacgga ggtaggggag ggt
//
LOCUS       DATAIR010000262        10300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_108839, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000262 DATAIR010000000
VERSION     DATAIR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108839"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VHM69_06315"
     CDS             <1..200
                     /locus_tag="VHM69_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740041.1"
                     /translation="WNPIGAAGAALLFGFAQAITFSVNEQAIPIEFIEMIPFVLTIIV
                     LAAFGGRAIAPAAIGKPYRKE"
     gene            complement(202..480)
                     /locus_tag="VHM69_06320"
     CDS             complement(202..480)
                     /locus_tag="VHM69_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2740042.1"
                     /translation="MDLPRLRRIRQGAVMSQEELAERSGVARDTISKLETGRRGAYPS
                     TIRKLAAGLEVRPQMLMGGVEYLEESPEGESGEKDLPDKPERDRKVGF"
     gene            583..1209
                     /locus_tag="VHM69_06325"
     CDS             583..1209
                     /locus_tag="VHM69_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX2740043.1"
                     /translation="MGLDPRLVVPYVTEQSPRGERTMDIYSRLLADRIIFIGMPVDDQ
                     VANVVMAQLLHLDSEDPEQDINLYINSPGGSVYAGMAIYDTMQFVNADVATTALGMAA
                     SMGSFLLTAGAKGKRSALPNARILLHQPHIGGGLGGQVSDVEIHARELVRTKRRMNEL
                     YAEMTGQPVEKIERDTDRDYMLGPEEAVEYGVIDRVVNRPAESVAAGR"
     gene            1193..2197
                     /locus_tag="VHM69_06330"
     CDS             1193..2197
                     /locus_tag="VHM69_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2740044.1"
                     /translation="MRQAGKMDVLILGGTVFLGRHLVEAALGRGHRVTLFNRGIHNPE
                     LFPDVEKIKGDRDGDLSSLEGRRWDAAIDTCGFVPRVVRASAGLLAVDHYTFVSSISV
                     YSDDIKPGADEDAPVQELSDPTVEEVTGETYGGLKALCEKAAEEEMPGRVFNVRPGLI
                     SGPHDPTDRFTYWPRRISAGGEVLVPDRPEIRVQFIDVRDLAAWIIEMAQEQRTGTYN
                     ATGPDYELTMGRFLEECRTVGGGGAEFVWVSEDFLLERGVEPFTELPLWVSREDAAML
                     AVDCGRAIAAGLAFRPLSETVKDVFEWDLARSADTESAAGLRPEKERELLRAWHGATL
                     "
     gene            2227..2634
                     /locus_tag="VHM69_06335"
     CDS             2227..2634
                     /locus_tag="VHM69_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740045.1"
                     /translation="MNLTHEYMHHRTGYRLGAGCCWIRIYKGTDSDAPVVVCEELPKV
                     GGVATEEVSGYLAAEVVREHFSGGLPDLPRPLLWIEHRPARRRGPGKYFLHTFPSYRP
                     RTVGAGFVRRVTLGASRRELLHPAEVAILTREV"
     gene            complement(2716..3018)
                     /locus_tag="VHM69_06340"
     CDS             complement(2716..3018)
                     /locus_tag="VHM69_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740046.1"
                     /translation="MITVTEEAKELFLNVEHPEGTILRLDPVIDQETGETQIGLAAGE
                     PRGDDQVVERDGESLLHIAAPVSEALDGSTLNMVDTPDGPAIGIGIPDDKPTTDDS"
     gene            3200..3394
                     /locus_tag="VHM69_06345"
     CDS             3200..3394
                     /locus_tag="VHM69_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740047.1"
                     /translation="MGEERGTTQYAIKQVGDRYYPVIIDREAGGHYEIKNPMTGGVLS
                     YNNPEAAEKYIERASQAEGS"
     gene            complement(3410..4249)
                     /locus_tag="VHM69_06350"
     CDS             complement(3410..4249)
                     /locus_tag="VHM69_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX2740048.1"
                     /translation="MIQLPGGTFLMGTDDGQGFPQDGEGPVRRIRLDPFFISPYATTN
                     DEFSTFVEETGYETEAERFGWSFVFYRFLSAKAKKKVLGAVDSAPWWRAVGGACWRHP
                     EGPGSGIKPRMNHPVVHVSYNDALAFCDWAGKRLPTEAEWEYAARGGLEQRRYPWGNT
                     LTPNQEHRCNIWQGEFPTSNTAKDGYKGTAPVDSFPPNGFGLYNVSGNVWEWCLDWFS
                     FHAGGPRENPSGPPTGSSRVIRGGSYLCHKSYCNRYRVAARSHNTPDSSTGNAGFRCA
                     ADT"
     gene            4612..6174
                     /locus_tag="VHM69_06355"
     CDS             4612..6174
                     /locus_tag="VHM69_06355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2740049.1"
                     /translation="MRGWRTSEKKPERAHRSATLTGALPRERWALLALLAIMLLGAAL
                     RFYGLGVQSLWGDELASWDFSERDALSQVIQGVRSDIHPPLYFLILHFTQWIFGDSEW
                     ALRLPSAIAGWLCIPAIYLLGKRLYSEREGIIAALLIAVLWAPIYFSQEARSYSMLIL
                     FSILTSYFWWDVMQNLRYRRELPKREAIWYVICAVLCAYLHYFGLILVVLQGVALAAL
                     AHGMLRKVVFLYVPVAVAYLLWLPSMIYQFQYSKQRGDWIGDPTLSALPDYFQFLFGR
                     SGLLSLAAWTLLSFLLIRGWDDLRPRRKGSGIPPGALLAAWALGPFVVAYAVSQSSVQ
                     LLTEKNLLISLPAVYLLLARSITRAFSGKAAAVFQGTVAVGLAAACLAYLLFSMDYYT
                     DPTKEQVRQAASYVVNYEGPDTLVVRCDVDDRLDYYLKTGERDDVEACEAEDFTKIEE
                     KVREGDYHEVFHFISHTDPEPQLISKLQRDFQPVHYERFRGAAVVVYKVRKHAPAGLP
                     RPEPPSSLPKRK"
     gene            6295..7935
                     /locus_tag="VHM69_06360"
     CDS             6295..7935
                     /locus_tag="VHM69_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2740050.1"
                     /translation="MGEPEDRGLTRKNFLKAGGGLLAGAYTLGLTGCEQEESTGAPQE
                     GASLAGRTASSRGPNILFLMSDQHRWDALGSVNPLIKTPHLDSLAARGVRFTQATCNA
                     PMCVPSRYSMMTGLYPSQVGVRHNTQMIPTDEGLPAPVLAQRLREMGFQTAGFGKTHW
                     YLGEKLAPNILTKTSTRGFEVRAQRNTDEPGRVEPGAIQMGHERPEDFAALRKESAPF
                     GSGETIPGYKGITSSVPADRHTEAWLTDEAVEFLENGRDESRPFFLYLSFDFPHAAFN
                     VPPEYEALYEMDEIPARPEPPPGTNLSGHAEEWQWDEWLTRTTPQERRRTTLRYYALC
                     TYVDAEFGRVLKKIEEMGETENTFVIFCSDHGEMLGDRHRYSKYCLYDASVRVPMIVA
                     GSGVPDELHGTTDDRHAELVDVLPTLLSVAGEATPPEYPGVSLLSDPVRAAGFAEMHG
                     TGYEPEQRAPAYMWRTRDWKLISYLPGYAANARAPSQTGELYDLRTDPDEYHNLYDAP
                     DHLKTRERLKGELMMHLALVWAGFPRQPAKAPLDSPSV"
     gene            7954..8889
                     /locus_tag="VHM69_06365"
     CDS             7954..8889
                     /locus_tag="VHM69_06365"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2740051.1"
                     /translation="MYRKESASSSLPTLPRGVLAAVLTPMDEDLAPNHRAFTAHCHRL
                     LAAGCHGLSVFGTTGEGTSLSTDERLAALEALVENGISGDTLLPGTGSCALTDTVRLS
                     RAALEAGAAGVLVLPPFYYKDTGDDGLFRFFAEVVERVGDDRLRLYLYHIPQMTGIDL
                     GLPLISRLIDAYTGVIVGTKDSSGDRERITTLCREFPGFSVLAGTETLLLDTLRSGGD
                     GCISATVNVTSRLARRVHDAHASGKVDEADALQERLTDLRTSIEAYPVIPALKFLMGE
                     LTGDGAWRNLRPPLSGLDESRAKELLSGLPLSELL"
     gene            complement(9025..>10300)
                     /gene="ligA"
                     /locus_tag="VHM69_06370"
     CDS             complement(9025..>10300)
                     /gene="ligA"
                     /locus_tag="VHM69_06370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX2740052.1"
                     /translation="MLSALKRYGLRVNPYEVHDDIDSVIEECERWAKKRESVGYQIDG
                     VVVKVDSREQQLALGSVARSPRWAIAYKFEPLAGRTKLLDIHVKVGRTGALTPQAVLE
                     PVNVGGVVISRATLHNEDYVKEKGILIGDTVVVERAGDVIPQVVKAVIEERDGDERTF
                     EMPKDCPVCGEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKSAMDIDGLGEKLATR
                     LFDLGFIKDAADIYDLEAEKLVPLEGFGEKSAQNLIQAIQRSKEQPFSRVLYALGIRH
                     VGSVTATLIATRFGGEDMMSGIDVERLTGIDGVGEVVARAVVEYFGLEDNRDLVKRLM
                     QRGLDFGREEDGTSDGPLSGKRVVITGTLDRPRSHYVEQLEAAGGTFTSSVSKNTDYV
                     LAGEEAGSKLEKARELGVPILDEAGFEELLSS"
BASE COUNT         1869 a         3281 c         3225 g         1925 t
ORIGIN      
        1 gctggaaccc catcggcgcg gcgggagcgg ccttgctctt cggcttcgcc caggccataa
       61 ctttcagcgt caacgaacag gccatcccca tcgagtttat cgagatgatc ccatttgtcc
      121 ttaccataat agtcctcgcc gccttcggcg gacgcgctat agcacccgct gccatcggca
      181 agccgtacag gaaagagtaa tctagaaccc gaccttcctg tcccgttcgg gcttgtccgg
      241 aagatccttc tcgccgctct ctccttcagg actctcttcc agatactcga cgccgcccat
      301 gagcatctgt ggccgtactt cgagtcccgc ggcgagcttg cgaatcgtgg acggatacgc
      361 cccacgccgc cccgtctcca gcttcgagat ggtgtcccga gccaccccac tcctctcagc
      421 caactcctcc tggctcatca cagccccctg ccgaatgcgc cgcaaacgcg gaaggtccat
      481 gctccttacc tccaaaacac tgcaaataac ttgcagaaac actacaacaa tgttacgcta
      541 ttgcaagtat agcctgttgg cggaagcaga gggagtttga tgatgggttt ggatccgagg
      601 ctggtcgttc cttatgtaac tgagcagagt ccgcgtggag agcggacgat ggacatctat
      661 tcgcggcttc tggctgacag gatcattttt atcgggatgc cggtagacga tcaggtagcg
      721 aacgtggtga tggcgcagct tttgcacctg gattccgagg accctgagca ggacattaat
      781 ttgtacatca attcgcctgg cggaagtgtt tacgcgggga tggcgatcta cgacaccatg
      841 cagttcgtca acgcggacgt ggcgacgacg gcgctgggga tggcggcttc gatggggtct
      901 ttcctcttga cggccggggc gaagggcaag cggagcgcgc tcccgaacgc gcggatactg
      961 ctgcaccagc cgcacatcgg cggtggtctc ggcgggcagg tctcggacgt ggagatccac
     1021 gccagagagc tcgtgcggac caagcggcgg atgaacgagc tgtacgccga gatgacgggg
     1081 cagccggtcg agaagatcga acgcgacacc gaccgtgact atatgcttgg accggaagag
     1141 gcggtcgagt acggcgtcat cgacagggtc gtcaaccgcc cggcggagag cgttgcggca
     1201 ggcaggtaag atggacgtac tgatcctggg cggcacggta ttcctcggac ggcatctcgt
     1261 cgaggctgcg ctcgggcgcg gtcatcgggt cacgttattc aacaggggaa tccataaccc
     1321 ggagctcttc cccgatgtag agaagatcaa gggcgacagg gacggtgacc tctcgtcgct
     1381 cgaagggcgg cgctgggacg cggcgatcga cacctgcggc ttcgtgcccc gcgtagtcag
     1441 ggcgtcggcg gggctgctcg cggtcgacca ttacaccttc gtctccagta tctccgtcta
     1501 ctccgacgac atcaagccgg gggctgacga ggacgcgccc gtgcaggagt tatctgaccc
     1561 gacggtcgag gaggtcacgg gggagacgta cggggggctc aaggcgctgt gcgagaaggc
     1621 tgccgaggag gagatgcctg gcagggtatt caacgtcagg ccaggtttga tctcgggtcc
     1681 ccacgatccc acggaccgtt tcacgtactg gccgcgcagg atctccgccg gcggcgaggt
     1741 tctcgtcccc gaccgcccgg agatcagggt tcagttcatc gacgtgaggg acctggcagc
     1801 gtggatcata gagatggcac aggagcagcg gactggtaca tacaacgcga cagggccaga
     1861 ttacgagttg acgatgggac ggttcctcga agagtgcagg accgtcggtg gcggcggggc
     1921 ggagttcgtc tgggtctccg aggacttcct tctggagcgg ggggttgagc ctttcacgga
     1981 gctgccgttg tgggtgtctc gggaggatgc cgccatgctg gccgtcgact gcggcagggc
     2041 catcgctgcg gggctcgctt tccggccctt gtccgagacg gtaaaggacg tgttcgaatg
     2101 ggaccttgcc cggagtgcgg atacagaatc ggctgctggt ctgcggcctg agaaggagcg
     2161 ggagttgctt cgagcctggc acggtgccac cctgtagcag gcgggcttcg cgaagagcga
     2221 gatggaatga acctcacgca cgagtacatg caccacagga cgggctacag gctcggggcg
     2281 gggtgctgct ggatccggat ctacaaaggt accgacagcg atgcgccggt agtcgtgtgc
     2341 gaggaactac cgaaggtcgg aggcgttgcc acggaggagg tctcagggta cctggcggcc
     2401 gaggtcgtca gggagcactt ctctggcggc ctgcccgacc tgcctcgtcc tctgctctgg
     2461 atcgaacacc gcccggcccg caggcgcgga ccaggcaagt acttcctcca cactttcccc
     2521 tcttaccgtc ccaggaccgt gggcgcgggc ttcgtgcggc gcgtcaccct cggggcctcg
     2581 cgccgcgaac tcctccaccc ggcagaagta gccattctca cacgagaggt ctagaaggca
     2641 cggcttcgca tgaggcagga gccgccggta aaggttcggc ggctcctgcc tacacgataa
     2701 ataaggtcgg agctactaag agtcgtccgt agtaggcttg tcgtcgggga tgccgatacc
     2761 gatggccgga ccgtcggggg tatccaccat gttgagcgtg ctaccgtcga gggcttcgct
     2821 cacgggggcg gcgatgtgca ggaggctctc gccgtcgcgc tcgacgacct gatcgtcgcc
     2881 gcgaggctcg cctgctgcga gccctatctg ggtctcaccc gtttcctggt ctatcacagg
     2941 atcgaggcgg aggatcgtac cctccgggtg ctccacgttc aggaagagct ccttcgcttc
     3001 ttcggttacc gtgatcatgc cttctcctct ctcgccgaca ccgcaagagg ccgcaacgcc
     3061 ggtctggagc ttgcattacg gtcaaacacc ctaccatttc aacgtctgat ttaaaccaca
     3121 acctcaacct gggcttaggt cttggcgacg cacggcgcgg taggacgtat agtgaccgcg
     3181 agcgtccgaa ggaggcgagt tgggcgagga gagaggtact acgcagtacg ccataaagca
     3241 ggtcggagac cgctattatc cggtcattat cgacagggaa gccggtggtc actacgagat
     3301 aaagaacccc atgaccggcg gcgttctcag ctacaacaac ccggaagccg cagagaaata
     3361 catagaacgc gccagccagg cagaagggtc gtagacgctg gtgccgccct tatgtatccg
     3421 ccgcgcagcg aaagcctgcg ttacccgttg agctgtcagg ggtattgtgg ctgcgcgccg
     3481 ccacccggta gcggttgcaa taggacttgt ggcacagata ggagcctccg cggatgaccc
     3541 tggaggatcc cgtgggtggt cccgaggggt tctcgcgcgg tccaccggcg tggaaggaga
     3601 accagtcaag gcaccactcc cacacgttgc cagagacgtt gtagagtccg aagccgttcg
     3661 gagggaagga atccacgggc gccgtgcctt tgtagccgtc ttttgcggtg ttgctggtcg
     3721 ggaactcccc ctgccagatg ttgcagcggt gctcctggtt cggggtcagc gtgttgcccc
     3781 aggggtagcg cctctgctcg agaccgcccc tggccgcgta ttcccactcg gcctcggtcg
     3841 gcagcctctt tcccgcccag tcgcagaagg ccagggcatc gttgtacgag acgtggacaa
     3901 cggggtggtt catgcggggc ttgatgccgg agcccggacc ttccggatgc ctccagcaag
     3961 ctcccccgac ggccctccac cacggggcgc tgtcgaccgc cccaagcact ttcttcttcg
     4021 ctttcgctga gagaaacctg tagaagacga aagaccagcc gaacctctcg gcctccgtct
     4081 cgtaacccgt ctcctccacg aacgtagaga actcgtcgtt cgtagtcgcg tatggggaga
     4141 taaagaaggg gtccaacctg atcctacgga ccggcccctc accgtcctgc gggaacccct
     4201 ggccgtcgtc tgttcccatc aggaaggtgc ctccaggaag ctggatcatg ccttccaata
     4261 acgtattctc cgcccgtaca cccgttcgcg gttttccaga cgattccccc gaagccagcc
     4321 gcgcgggcgc gcaacaagaa ggtgggatct cgtgctgtgg ggttcgacct gcccactttc
     4381 gtgcgccatc tcccgcgctc atcggctgta cgatacctcc gtcgttttcg ttgacccata
     4441 gggtatcaat gaagggagcg ttgccaatgg gcacaccaat gccaatttgc gtgctcccaa
     4501 gagttgtgtg ttcagttcat ttcgatcagg acgttgatgt agcttgagaa cctgtttaac
     4561 atctcagaac gtccactacc cgccaccgca cgagacaggg aggagcgtac ggtgcgagga
     4621 tggagaacga gcgagaagaa gccggagcgc gcccaccgca gcgcgacctt aaccggcgcg
     4681 ctccctcggg agagatgggc gcttctggcg ctgctggcga tcatgctcct cggcgcggcg
     4741 ttgcggttct acggccttgg cgtccagtcg ctgtggggag atgagcttgc gagctgggac
     4801 ttcagcgaac gggacgcgct ctcgcaggtc atccagggcg tacgtagcga catccaccct
     4861 ccactgtact tcctcatcct ccacttcacg cagtggatct tcggcgactc cgagtgggcg
     4921 ctgaggctgc cttcggccat cgcagggtgg ctgtgcatac ctgcgatcta cctcctcggc
     4981 aagaggctct actccgagcg cgaggggatc atcgccgcgc tgctgatagc ggtgctgtgg
     5041 gccccgatct acttcagcca ggaggcccgc tcttactcga tgctgatcct gttctccatc
     5101 ctcacgtcct atttctggtg ggatgtaatg cagaatctgc gctaccgcag ggagctgccg
     5161 aaacgagaag ctatctggta cgtcatctgc gccgtactct gcgcctacct gcactacttc
     5221 gggttgatcc tcgtggtgtt gcagggcgtc gcgctggcgg cgctcgccca cgggatgctt
     5281 cggaaggtcg tattcctata tgtccctgta gccgttgcct acctcctctg gctgccgagc
     5341 atgatctacc agtttcagta cagcaagcag aggggggact ggatcgggga tccgaccctc
     5401 tcagccttgc ctgactactt ccagttcctc ttcggccgct ccggtctact ctcgctcgcc
     5461 gcgtggacgc tcctctcctt cctcctcatc cgcggttggg acgatctgcg gcctcgccgg
     5521 aaggggagcg ggatcccgcc gggcgcgctg ctcgccgcgt gggcactagg gccattcgtc
     5581 gtggcctatg cggtgtcgca atcttccgtc cagttgctaa ccgagaaaaa cctcctgatc
     5641 tcgcttcctg ccgtctacct gctcctcgca cgctccatca cccgcgcgtt ctcgggcaag
     5701 gccgcggcgg tcttccaggg gaccgtggca gtggggcttg ccgccgcgtg cctcgcgtac
     5761 ctgctcttct cgatggacta ctacaccgac ccgaccaagg aacaggtccg ccaggccgcc
     5821 tcctacgtcg tgaactacga aggcccggac accctcgtcg tgcgctgcga cgtcgacgac
     5881 aggctggact actacctcaa gacgggagag agggatgacg tggaagcctg cgaggctgaa
     5941 gactttacga agatagagga aaaagttcga gagggggact atcacgaggt cttccacttc
     6001 atctcccaca cagacccgga gccgcagttg atctccaagc tccagaggga cttccagccg
     6061 gtccactacg agcggttccg cggcgccgct gtggtcgtgt acaaagtgag gaagcacgcg
     6121 cccgcaggac tcccgcggcc cgaaccacca tctagcctcc cgaagcgcaa gtgacccgca
     6181 ctcgcaacga cgcttgccgt cctcaggggg ccggtatacg atgggccgtc gatcccggcc
     6241 ttccccgaat gggtgctctg aaggccgaag ctacgatgcg cgagaggaca gggcgtgggt
     6301 gagcccgaag accgcggtct gacgcggaag aattttctga aagcgggcgg aggtctgctc
     6361 gccggcgcgt acaccctcgg gttgaccggt tgcgagcagg aggagtccac aggggcgccg
     6421 caagagggcg cgagcctggc cggaaggacc gcctcttcac gaggtccgaa catactgttc
     6481 ctgatgagcg accagcaccg ctgggacgct ctagggagcg tgaaccctct gatcaagact
     6541 ccccatcttg attccctggc cgcacgtggc gtccgcttca cccaggccac ctgcaacgct
     6601 ccgatgtgcg tgccgagccg ctattcgatg atgacgggcc tgtatccctc gcaggttgga
     6661 gtccgccaca acacccagat gatccccacc gacgagggcc tgcctgcgcc cgtgctcgcc
     6721 cagcgcctgc gcgagatggg cttccagaca gccgggttcg gcaagaccca ctggtacctg
     6781 ggtgagaagc tcgcccccaa catcctcacg aagacctcta ccaggggatt cgaggtccgt
     6841 gcccagagga acacggacga gccggggagg gtggagccgg gggcgatcca gatggggcac
     6901 gaacgtcccg aagactttgc cgccctacgg aaggagtcag cgcccttcgg ttcgggggag
     6961 actatccctg gctacaaggg catcacgagc tccgtgcccg cagacaggca caccgaggcg
     7021 tggctcacgg acgaagccgt cgagttcctg gagaacggca gggacgagag cagacccttc
     7081 ttcctctacc tctccttcga cttcccgcac gcggccttca acgtcccgcc cgaatacgaa
     7141 gctctttacg agatggacga gataccagcc cgacccgagc cacctccagg gacgaacctc
     7201 tcgggccacg cggaggagtg gcagtgggac gaatggctga cccggacgac gccacaggag
     7261 cgcaggagga cgaccctccg ctactacgcc ctctgtacct acgttgatgc cgagttcggc
     7321 agggtgctga aaaaaataga ggagatgggg gagacggaga acaccttcgt catcttctgt
     7381 tcggaccacg gcgagatgct cggcgaccgt caccgttaca gcaagtactg cctctacgac
     7441 gcgagcgtga gggtgcccat gatcgttgcc ggctcgggtg taccggacga actccacggc
     7501 accacagacg accgtcacgc agagcttgtg gacgtgctgc cgacactcct ctcggtcgct
     7561 ggagaagcga ctccacccga atatcccggc gtgagcctgc tctccgatcc ggtacgtgcg
     7621 gccggtttcg ccgagatgca cgggacgggc tacgagccgg agcagagagc cccggcatac
     7681 atgtggcgca cccgcgactg gaagcttatc tcgtacctgc ccggctacgc ggcgaacgcg
     7741 cgagcgccct cacagacggg cgagttgtac gacctgcgca cagatcccga cgaataccat
     7801 aacctctacg atgcccccga ccacctgaaa actcgtgagc ggctcaaggg cgagctaatg
     7861 atgcaccttg ctctagtttg ggcaggcttc cccaggcagc cggcgaaggc gccgctagac
     7921 tcaccgtccg tctgactttg ttgagaggag atcatgtacc gcaaagagag cgcgagcagt
     7981 tcacttccaa ccctgccgag aggagtgctc gcggccgtcc tcacccccat ggacgaggac
     8041 ctggcgccca accatcgggc cttcacggct cattgccaca ggctgctagc ggcgggctgc
     8101 cacggcctgt cggtcttcgg gacgaccggg gagggcactt ccctctccac ggacgagcgg
     8161 ctcgcggctc tcgaagcgct cgtcgagaat ggcatctccg gcgacaccct gctgccgggt
     8221 acaggttcat gcgccctcac cgacaccgtc cgcctgagcc gggcggcgct cgaggcgggc
     8281 gcggccggcg tgcttgtgct gccgcctttc tactacaagg acacaggcga cgatggtctg
     8341 ttccgcttct tcgccgaggt cgtcgagcgc gtcggcgacg accgtctgcg cctgtacctc
     8401 taccacatcc cgcagatgac gggcatcgac ctcggacttc ccctgatctc acgcctcatc
     8461 gacgcctata caggcgtgat cgtcggcacg aaggacagct ccggcgacag ggagcgcatt
     8521 acgaccctct gcagggagtt ccccggcttc tccgtcctcg caggcacgga gacactcctg
     8581 ctcgacaccc tgcgtagcgg cggcgacggt tgtatctccg ccaccgtcaa cgtcacctcc
     8641 aggctcgccc gtcgcgtcca cgatgcgcac gcctcaggca aagttgacga ggcggatgca
     8701 ctgcaagagc gtcttacgga tctccgtact tccatcgagg cgtatcctgt gataccggcg
     8761 ctcaagttcc ttatgggcga gctgaccggc gacggggcat ggcgcaacct gcgtccgcct
     8821 ctctccgggc tggacgaaag tcgcgcgaag gaacttctct cagggctgcc gctctccgag
     8881 ttgctctgac tagaaagttg cagaaggtgc tgcttcggac gcagaggcga acgcgaataa
     8941 acgttccgcc aggctcttcc cgtcgcgcct gcgcagcagg gccgcaacaa gaacgttccc
     9001 tacctgatgc ctgaagcctg cggttcagga ggagagcagc tcttcgaatc ccgcttcgtc
     9061 gaggatggga acgccgagct ctcttgcctt ctctagcttc gagccggcct cctcgcctgc
     9121 gagcacgtag tctgtgttct tgctgacgga ggaggtgaat gtaccacccg ccgcttcgag
     9181 ctgttcgacg tagtggctgc gaggacgatc cagggtccct gtaataacga cgcgcttgcc
     9241 tgagagtggt ccatccgagg tgccgtcctc ctcgcgcccg aagtcgaggc cgcgctgcat
     9301 gaggcgcttg acgaggtcgc ggttgtcctc caggccgaag tactcgacga cggcgcgggc
     9361 gacgacctcc ccgacgccgt cgatcccggt cagccgctcc acatcgatgc cgctcatcat
     9421 gtcctcgccg ccgaagcgcg ttgcgatgag ggtggccgtg accgatccga cgtggcggat
     9481 tccgagggcg tagaggacac gggagaacgg ctgttccttg ctcctctgga tggcctggat
     9541 caggttctgc gcgctcttct ctccgaagcc ttcgaggggg acgagctttt cggcctccag
     9601 gtcgtagatg tcggcggcgt cctttatgaa gccgaggtcg aagaggcggg tggcgagctt
     9661 ctcgccgagt ccgtcgatgt ccatggctga cttcgaggac cagtggatta tgtgctcgag
     9721 ggcctgggcc ggacagcggg cattcacgca gcgggtaacg gcttcgccct cggggcgcga
     9781 gacaggctcc ccgcagactg ggcagtcctt cggcatctcg aaggtgcgtt cgtctccgtc
     9841 gcgttcctct ataacggcct tgacgacctg cgggatcaca tcccccgccc gctcgacgac
     9901 gaccgtgtcg ccgatgagga tacccttctc cttgacgtag tcctcgttgt gcaatgtagc
     9961 ccgcgagatc acgaccccgc cgacgttcac gggctcgagc actgcctgcg gcgtgagcgc
    10021 gcccgtccga cccaccttca cgtggatgtc gagcagcttc gtgcgccctg ctagcggctc
    10081 gaacttgtac gctatcgccc accgcggaga cctggcgacg gagccgaggg cgagttgttg
    10141 ctcgcgcgaa tcgaccttca cgacgacgcc gtcgatctga taaccgacag actcccgctt
    10201 cttcgcccac cgctcgcact cctcgatcac ggagtctatg tcgtcgtgga cctcgtatgg
    10261 gttgacgcgc agtccgtatc tctttagcgc ggagagcatc
//
LOCUS       DATAIR010000263         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_146405, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000263 DATAIR010000000
VERSION     DATAIR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146405"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHM69_06375"
     CDS             <1..293
                     /locus_tag="VHM69_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740053.1"
                     /translation="SIMFGEVEELKQIAEERVQELHDKGYSQAYVWGTPEVGGTGGIK
                     GNHSKFILMDEPEVYNLPRHPYLPQEKVMGGTIASMVAATVLGAVVALAFRD"
     gene            304..1341
                     /gene="nrfD"
                     /locus_tag="VHM69_06380"
     CDS             304..1341
                     /gene="nrfD"
                     /locus_tag="VHM69_06380"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX2740054.1"
                     /translation="MSETTRDQVQQSNGKVETSHHPETDPERKGKNYYGIPPIKHAHW
                     TWQIPVYFWIGGIGAGVHMFSTIAQILGHKDDDLTRISRYTVLATMMISPVLLIWDLG
                     RPERFYNMLRILKLRSPMSTQSWSVFAFGNLSGLISARQAAEDGLLGEDNLLVRLVKL
                     IPARFLSILGLPVGLNVGSNTGNLISATSVPMWARNWLFMGPTFLASGLSTALSWLSL
                     VLHLTNSGEEKTLRVLRRAEKAALVIEAGLIAASLVRMSKWSKPLFSREVAPLFVGGT
                     LLGGILAPMALLFGKQSRSKSLLASVLALAGGLAFRFAIIKGGRISADDPEAYFTFAS
                     AENAPQPEDKI"
     gene            complement(1351..1749)
                     /locus_tag="VHM69_06385"
     CDS             complement(1351..1749)
                     /locus_tag="VHM69_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740055.1"
                     /translation="MRVVTAKDMQTGRGPEDWFTGTTWRDAAPVGSSPDVAVNRVFFE
                     PGARTHWHTHTEGQILYIVTGTCRVGNEGEPPVEVEAGGVVYFAPDEKHWHGATPDTY
                     MVHMAINVATTTDGGTDWFDPVTDEEYAGG"
     gene            complement(1836..3485)
                     /locus_tag="VHM69_06390"
     CDS             complement(1836..3485)
                     /locus_tag="VHM69_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2740056.1"
                     /translation="MAPELNLGPLLRYTGESDATVWVESDAACEVEVLGHKTRTFEIE
                     GHHYALVEITDLEPGETYEYQVSLDGAKVWPDDEHDFPPSVIRTIAPDGELNLSYGSC
                     RVSVPHEPPYTLPADAHKEAYERDALQALALRMMNEPHERWPDALLLLGDQIYADEVS
                     EGALEFIHSRRDTSEPPGEQIADFEEYTRLYWDAWRDPVTRWLLSTVPSAMIFDDHDV
                     DDDWNISETWVRQKRSTPWWEERIIGAFMSYWIYQHLGNLSPRELEGDGLFQKVREAE
                     NATRILREFAYKADREVAGTRWSYHRDFGRVRLIMMDSRAGRVLDEGHRAMLDAEEWD
                     WIVEKATGDFDHLLFGTSLPFILGPGMHHLEAASEAVCAGAWGRPAARIGESVRQLID
                     LEHWAAFNDSFTGLGHLLRSVAAGERSNGDPPASITVLSGDVHHGYLAKMDFGDDVKT
                     PVYQSASSPLRNPLGLPERLVMRLGWSEKGKRIGEFLSRLAGVEEPDFNWRLMHEEPW
                     FHNHVSSLEIRGRKARLKVEKTTPEDNDEPYLYRILEHRLA"
     gene            complement(3514..4278)
                     /locus_tag="VHM69_06395"
     CDS             complement(3514..4278)
                     /locus_tag="VHM69_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX2740057.1"
                     /translation="MHHPDPAQPSRGAKDLGYIAEERSKLGPFSRGERNTLIAFIVAV
                     SLWVLPGLSAVIFGDESTVYSVLYSRLDEGTVAIIGAALLFILPINWAERRFTMNWNQ
                     AARIDWGTIILFGCGIALGTLLSDTGLAETIGTGVADALGFSSLVAVSAVSALIAILI
                     SETTSNTASATIVVPIVIPIAAAAGLDPLVPALAAVFGASFGFMMPVSTPQNAVVYGS
                     GMIPITKMVRSGIVFDIVGLILIVLLIPVMAGLIIA"
     gene            complement(4221..4856)
                     /locus_tag="VHM69_06400"
     CDS             complement(4221..4856)
                     /locus_tag="VHM69_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX2740058.1"
                     /translation="MTAVLALALCVLFNVPSVGPNAEDAPGDIVFAGFADPVIFLFIG
                     AFIIAWAMITHGLDRRFACLVLSLPGVGRSTYGVIIAFGFIAASISAFVSNTTTAAML
                     LPIGLGMMATLGGMVTEQSDTDKDVSRLRFGTALMLMISYAAGVGGLLTPIGTPPNLI
                     GIGFIEEETGVRFSFFDWVLTAFPIVVLMFIALCTILILLNHPEVRRISGT"
     gene            complement(4881..5096)
                     /locus_tag="VHM69_06405"
     CDS             complement(4881..5096)
                     /locus_tag="VHM69_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740059.1"
                     /translation="MSEDVRPQGTYRTLDEQREVLTPAEERFERARQTTGLILGPVAF
                     LIMYFLPLPLPRDQHTLAAILVFTIIY"
     gene            complement(5165..6253)
                     /locus_tag="VHM69_06410"
     CDS             complement(5165..6253)
                     /locus_tag="VHM69_06410"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2740060.1"
                     /translation="MESGFGATAPYTVGVEEEFQLVDPGTRALTPAIDEVLAAGEGTQ
                     LITSELSQSCVEMLSPVFTSVSDLARRLPGLRREVRDLARSRGVEIVAAGTHPFSEPT
                     EQPLTSGDRNRRVEEEMGWVARTQAIYGLHVHVAVPDGDAAIRAVNALARHVPLLVAL
                     SANSPFWRRFDTRLASTRIKIFELFPRSGLPPVFRSWEDFENHVETLVASGSMPDYTW
                     CWWDARPHQRFGTVELRSPDVQTDASYTASLAALVQCLVVSADEYPPENPLLIAENKW
                     RAVRYGMDAGFHDFVAGREVQARSIARGLVEELRPVAQELGCEEELGGVLEIVELGTG
                     AELQRATLDRSGSLEGVVDYLIEGTAPD"
BASE COUNT         1139 a         1971 c         2042 g         1159 t
ORIGIN      
        1 actccatcat gttcggcgag gtcgaggagt tgaagcagat tgccgaggag cgggtgcagg
       61 agctccacga caaaggctac agccaggcct acgtgtgggg cacgcccgag gtgggaggga
      121 caggaggcat aaaggggaac cattccaagt tcatcctcat ggacgagccc gaggtctaca
      181 acctgccccg ccatccctat ctgccgcagg agaaagtcat ggggggaaca atagccagca
      241 tggtcgctgc gacggtgctc ggcgcggttg tggcgctggc tttcagggac tagggggaga
      301 ccggtgagcg agacgacgag ggatcaggtc cagcagagca acggtaaggt cgagacgtct
      361 caccatcccg agacggaccc ggagagaaag ggcaaaaact actacggcat cccgcccata
      421 aagcacgcgc actggacgtg gcagatcccc gtctacttct ggatcggcgg tataggggcc
      481 ggggtccaca tgttctcaac gatagcccag atcctcggcc ataaagacga cgatctcacc
      541 cgcataagcc gctatacggt gctcgccacc atgatgatca gccctgtgct tttgatctgg
      601 gacctcgggc gtcccgagcg cttctacaac atgctccgaa tcctcaagct ccgttccccg
      661 atgtccaccc agagctggtc ggtcttcgcc ttcggcaacc tgagcggtct gatctccgcc
      721 cgccaggccg cagaagacgg attgctcgga gaagataatt tgctggtgcg actggtaaag
      781 ctcatccctg cgcgcttcct ctctatcctg ggcctgcctg tcgggctcaa cgtcggctcg
      841 aacacgggga acttgatctc cgcgacctcc gtcccgatgt gggcgcgaaa ctggctgttc
      901 atgggaccga cgttcctcgc ctcggggctc tcgacggctc tctcgtggct ctcactcgtc
      961 ctccacctga cgaactcggg cgaggagaag acccttcgcg tgcttcgtcg cgccgagaag
     1021 gcggccctcg tcatagaagc agggctcatc gccgcctccc tggtaaggat gagcaagtgg
     1081 agcaaaccgc tcttctccag agaggttgcc ccgctcttcg tcggcggaac cctcctcggc
     1141 ggaatactcg cgccgatggc cctgctcttc ggcaagcaaa gccgctccaa aagtcttctc
     1201 gcttccgtgc tcgcgctcgc cggcggcctc gccttccgct tcgccataat aaagggaggc
     1261 aggatcagcg ccgacgaccc cgaagcgtac ttcaccttcg ccagcgccga gaacgccccg
     1321 cagccggagg acaagatcta gcgagcttct ctagccgccc gcgtactcct cgtcagtgac
     1381 gggatcgaac cagtccgtgc cgccgtcagt ggtggtggcg acgttgatgg ccatgtggac
     1441 catgtatgtg tctggagtgg cgccgtgcca gtgcttctcg tccggcgcga agtagacgac
     1501 gcccccggct tcgacctcga ctgggggctc gccttcatta ccaacgcggc atgtgcccgt
     1561 gactatgtag aggatctgac cttcagtatg cgtgtgccag tgcgtgcggg cgccaggctc
     1621 gaagaagaca cggttgacgg ccacgtccgg ggaggagcct acgggcgcag catccctcca
     1681 agtcgtcccc gtgaaccagt cctcgggtcc acgtccggtc tgcatatctt tcgcggttac
     1741 gactctcatg ggaaatctcc cttcggtcga tttcaggctt caggtctcag gctacaggtt
     1801 ccaggttttt cctgatgcct gacacctgaa gcctatcatg cgaggcggtg ttcgaggatt
     1861 ctatacaggt acggttcgtc gttgtcttcg ggcgtcgtct tctccacctt caggcgggct
     1921 ttgcggcccc ggatctcgag gctcgagacg tgattgtgga accagggttc ttcgtgcatg
     1981 aggcgccagt tgaagtccgg ttcttcgacg ccggccaggc gggagagaaa ctcgccgatt
     2041 cgctttccct tctccgacca gcccaagcgc atgacgagac gttcggggag tccgagaggg
     2101 ttccgcaacg gggagctggc cgactggtag acgggggtct tcacgtcgtc gccgaagtcc
     2161 atttttgcca ggtagccgtg atgcacgtcg ccggagagga ccgttatgga tgccggaggg
     2221 tcgccgttcg agcgttcgcc tgcggcgacg gaccgtagaa ggtggccgag gccggtgaag
     2281 gagtcgttga aggctgccca gtgctcaagg tcgataagct ggcggaccga ctcgcctatc
     2341 ctcgccgcag ggcggcccca ggccccggcg cagacggcct cgctggcggc ctcgaggtgg
     2401 tgcataccgg gacccagtat gaatgggagc gaggtaccga agaggaggtg gtcgaagtcc
     2461 ccggtggcct tctccacgat ccagtcccac tcctcggcgt cgagcatggc gcgatgaccc
     2521 tcgtcgagca ctcttcctgc gcgcgagtcc atcattatta gacggaccct gccgaagtcg
     2581 cggtggtagc tccagcgggt gcctgctacc tctctgtccg ccttgtaggc gaactcacga
     2641 aggatacggg tcgcattttc agcttcgcgc accttctgga agaggccgtc gccttcgagt
     2701 tcccgcgggg agaggttgcc gaggtgctgg tagatccagt aagacataaa cgccccgatg
     2761 atgcgctcct cccaccatgg cgtgctgcgc ttctgcctta cccaggtctc ggagatattc
     2821 cagtcgtcgt cgacgtcgtg gtcgtcgaag atcatggccg aaggcacggt cgagaggagc
     2881 cacctggtta cgggatctct ccatgcatcc cagtacaggc gtgtgtactc ctcgaagtca
     2941 gcgatctgct cgcccggcgg ctcgctcgta tcgcgccgcg agtggatgaa ctccagggcg
     3001 ccctcggaga cctcgtccgc gtagatctga tcccccaaca ggagcagcgc gtcgggccag
     3061 cgctcgtggg gttcgttcat catgcggagg gcgagtgcct ggagggcgtc gcgctcgtag
     3121 gcttctttgt gcgcgtcggc ggggagcgtg tatggcggct cgtgcggtac ggagacgcgg
     3181 caggacccgt aggagaggtt caactcaccg tcgggcgcga tcgtgcggat cacagacggc
     3241 gggaagtcgt gttcatcatc gggccagacc ttcgcgccgt cgagggagac ctggtactcg
     3301 tatgtctctc ccggttcgag gtccgtgatc tcgaccaggg cgtagtggtg accctcgatc
     3361 tcgaaggtcc tggtcttgtg gccgaggacc tcgacctcgc acgcggcgtc gctctcgacc
     3421 cagacggtgg cgtcgctctc tccggtgtaa cggagcagcg ggccgaggtt cagttcagga
     3481 gccatcccca gttcttaccc gccgccggtt ttcttacgct atgatcaaac cggccatgac
     3541 gggtatgagc aggacgatca ggatcaggcc gactatgtcg aagacgatgc ccgagcgaac
     3601 catcttggtt atcgggatca tgcccgagcc gtagacgacg gcgttctgcg gcgtgctgac
     3661 aggcatcatg aatccgaagg acgccccgaa gacggcggcg agcgcgggta cgagcgggtc
     3721 gagcccggcc gcggctgcta tcgggatcac gattggcacg acgatggtgg cgctcgcggt
     3781 gttcgatgtg gtctccgaga tgaggattgc gatgagcgcg gagaccgccg ataccgcgac
     3841 caggctcgag aagccaaggg cgtcggccac gcccgttccg atggtctcgg cgaggccggt
     3901 gtccgagagg agcgtcccga gcgcgatgcc gcagccgaag aggatgatgg tcccccagtc
     3961 aatcctggcg gcctggttcc agttcatggt gaagcgccgc tctgcccagt ttatcggcag
     4021 gatgaacagg agcgccgcgc cgatgatcgc gaccgtcccc tcgtcgagcc tgctgtagag
     4081 cacgctgtag actgtggact cgtcgccgaa gatcaccgcg gagagcccgg gcaggaccca
     4141 caggctgacg gcgacgatga aggcgatcag ggtgtttcgt tcgccccttg agaacgggcc
     4201 tagcttgctc cgctcctcag ctatgtaccc gagatccttc gcacctcggg atggttgagc
     4261 aggatcagga tggtgcacag cgctatgaac atgagcacga ctatcgggaa ggctgtcagg
     4321 acccagtcga agaagctgaa cctcacgccg gtctcctcct cgataaatcc gatgccgatg
     4381 aggtttggag gggtccctat gggcgtgagc agtcctccta cgccggcggc ataggagatc
     4441 atgagcataa gggccgtccc gaagcgtagc cggctcacat ccttgtcggt gtccgactgc
     4501 tcggtcacca tcccgccgag ggtcgccatc atcccgagcc ctatcggcag cagcatggcc
     4561 gccgtcgtgg tgttggagac gaacgcgctt atcgaggcgg cgatgaagcc gaaggcgatg
     4621 atcacgccgt aggtcgagcg ccctacgcca ggcagcgaga gcacgaggca agcgaacctg
     4681 cggtcgaggc cgtgtgtgat catcgcccag gcgatgataa atgccccgat gaacaggaaa
     4741 attacggggt cggcgaaacc ggcgaagacg atgtcgccgg gagcgtcctc ggcgttcggg
     4801 ccgacgctcg ggacgttgaa aagcacgcag agggccagcg ccaggaccgc cgtcacgggg
     4861 atggggattg cctcggataa ctagtagatg atggtgaaca cgagtatggc ggcgagcgtg
     4921 tgctgatccc tcgggagcgg aagcggaagg aagtacatga tcaagaacgc cacaggcccg
     4981 aggatgagcc ctgttgtctg ccgtgcccgc tcgaaccgtt cttcagcagg cgtcagcacc
     5041 tcacgttgct cgtcgagcgt cctgtacgtg ccctgcggtc ttacgtcctc actcatgcaa
     5101 tctcccccta taacacaagt gttccttccc atcgggaagt atagcgattg tcatacttgc
     5161 ggcgctagtc gggcgcggtg ccttcgatca ggtagtccac cacgccttct aaggagccgc
     5221 tcctgtctaa cgtggcccgc tgcaactcgg cccccgtgcc tagctccacg atctcgagca
     5281 cgccaccaag ctcctcctcg catcccaatt cctgcgcgac gggccgcaac tcctccacca
     5341 gcccgcgtgc aatggagcgg gcctgcacct ccctgcctgc gacgaaatcg tggaagcctg
     5401 cgtccatccc gtagcgcacg gcgcgccact tgttctcggc gatgagcaga ggattttccg
     5461 gcgggtattc gtcggcgctc accacgaggc actggacgag cgcggcgagg gacgcggtgt
     5521 aggaagcgtc cgtctgcacg tcgggcgaac gcagctcgac ggtgccgaac ctctggtgtg
     5581 gacgcgcgtc ccaccagcac caggtgtagt cgggcatgct gcccgaggcg acgagggtct
     5641 cgacgtggtt ctcgaagtct tcccaactcc ggaagacggg cgggaggccc gagcggggga
     5701 acagctcgaa gatcttgatc cgcgtcgagg cgagcctggt gtcgaacctt cgccagaaag
     5761 gtgagttggc ggagagcgcg acgaggagcg ggacgtgcct tgcgagtgcg ttgacggcgc
     5821 gtatcgccgc gtccccgtcg ggcacggcga cgtggacgtg cagcccgtag atcgcctgcg
     5881 tcctggcgac ccagcccatc tcctcctcga cgcgccggtt gcgatcccca ctcgtgagag
     5941 gctgctccgt cggctcggag aaagggtggg tgccagccgc gacgatctcg accccacggc
     6001 tgcgcgcgag atcccgcacc tcccgacgca acccgggtag cctgcgcgcg aggtcggaga
     6061 cggaagtgaa gacgggggag agcatctcca cgcatgactg cgagagctcc gaggtgatga
     6121 gctgcgtacc ctctcccgct gcgagcacct cgtcaatggc cggagtcagg gcgcgggtgc
     6181 cggggtctac gagctggaac tcctcctcga cccccaccgt atacggcgcc gtcgcgccga
     6241 atccgctctc catccacgta ctctagcagc catgggggat ggccggcgac cccagagtca
     6301 actcctgtct g
//
LOCUS       DATAIR010000264        10444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_53719, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000264 DATAIR010000000
VERSION     DATAIR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53719"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VHM69_06415"
     CDS             <1..612
                     /locus_tag="VHM69_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX2740061.1"
                     /translation="AYHEGGHAILGLLVPGADPVNRVTIVPRGQALGVTYQRPEDDRY
                     NYPEAYLRARIVGALGGRSAEEIVYGTKTTGAESDIEQATNLARRMVTRWGMSDELGT
                     VELAPRDNPYLGGSGGYGGERTFSEETARIVDSEVLRIINECHNEARRLLTEHRKELE
                     ALAAALLERETLDEEEILEVTGLPPAPPLENRKVPIPDADSHA"
     gene            663..1619
                     /locus_tag="VHM69_06420"
     CDS             663..1619
                     /locus_tag="VHM69_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2740062.1"
                     /translation="MTAENTANPAALNTIVLIHGLWMTPLSWEHWIDLYEARGFRVLA
                     PAWPGMGGDIDELRRDPSGIEHLGIREIVDHYAAIIRELVCPPIIMGHSFGGAFTQIL
                     LDRGLGAAGVAIDSAAVKGVLTLPPWSTLKSGFPVLKSPANNHRAVALSLEEFRYAFT
                     NTLSEEESQAAYERYAVPGGYSSRLHWRPSTHAPQLMSTSTTTTAHLCCSLQEGRITS
                     VPPRSTSPTSDVISSPRRSRCTRSSLSALISRWARRAGSRSTPSPGPSRTLLCTSCPR
                     PPNRPHLGYTSTSALTRRNSRRRVTGCSVKMRFRTYDRRHRI"
     gene            1788..2075
                     /locus_tag="VHM69_06425"
     CDS             1788..2075
                     /locus_tag="VHM69_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740063.1"
                     /translation="MRGLPLGTSDGPAWLIGEFIGSDILINNAGVIRPRPGGFLSLTD
                     EDWMWGLNMSFLVAVRTTCAALQHLLDHGAGTNVNTCSVHAVQPKPPVIDT"
     gene            complement(2223..2441)
                     /locus_tag="VHM69_06430"
     CDS             complement(2223..2441)
                     /locus_tag="VHM69_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740064.1"
                     /translation="MKVAAAPDESVALLMEGVLKEVGIPSLIQRAAGFDAPDFLAAGP
                     RDVLVPGSLVEEAKQILEDTTGLGSYAP"
     gene            complement(3307..4218)
                     /locus_tag="VHM69_06435"
     CDS             complement(3307..4218)
                     /locus_tag="VHM69_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2740065.1"
                     /translation="MNYLVALTALGGRDALSLGRMLARAGDVTLTVCVVVPQTWDYPS
                     PARVDAEYAAFLKQYAEDAIAEAREFLGDEVRAEYTSISASSASEGLIATATEKGAAL
                     IALGSARYGPLGRITVGGIANEMLHVSPVPVALAPRGYRLPSDTRLRRVTCAFSGSMQ
                     SRSAFNAALQLSSRHGVPLQLTTFVVRDRQMYPSQVGYDAERLVAEQWRAQALEAQEK
                     ALATLPDNVTEVEAGIVEGRDWDDALDSLPWEEGEVLVVGSSRLGPVARVFLGSNSTK
                     IVRSSPVPVVVIPRGADVHIAEDAPHA"
     gene            complement(4215..5666)
                     /locus_tag="VHM69_06440"
     CDS             complement(4215..5666)
                     /locus_tag="VHM69_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2740066.1"
                     /translation="MSGTRMRRGSLFRRKPVEAFVTETGPDAEGGELSRSIGLFQLTM
                     FGVGATIGTGIFIVLTEAVPEAGPAVIVSFALAGITAALTAVCYAELASTIPVSGSSY
                     SYTYATMGEIVAFGVAACLLLEYGVSAAAVSVGWGQYLNELLFDVFGFRIPGAINAAP
                     GEGGFINLPAVVLVTLCGLLLIRGVRESATANTIMVLIKLAVLALFIVVAFTAFSAEN
                     LEPFAPFGIAGIGAAAGSIFFSFIGLDAVSTAGEEVHNPRRNLPLAIIFATIIVTTFY
                     ILVALAGVGAQPYQQFAGQEAGLSVIMRNITGSGWPAAVLALGATISIFSVTLVVLYG
                     QSRILFAMGRDGMLPEVFHRVDPRTLAPVRNTIIVCAFVGLLAGLVPLGVLIDLTSMG
                     TLVAFTVVSIGVIILRRTQPDLPRGFRVPGYPVTPILSAAFALYLISGLPYSTFALFA
                     VWLGVAAIIYFTYSARHSRLARGIDVATEEEPR"
     gene            6164..7027
                     /locus_tag="VHM69_06445"
     CDS             6164..7027
                     /locus_tag="VHM69_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740067.1"
                     /translation="MADPVALQTLGFESCSNGWWKTIGERRLRFTRLISHLDLEKTER
                     LQYGVFGVSDRDLASSSILVTVHETGGEVLGAFDGEQLVGFVSGWGGYVDGRARLVSD
                     LMAVEAGYRGGIGFTLKALQAVVASQAGFEEIVWTVDPLRAANARLNFERLGAHSRKY
                     MRNVYGEHFGEGLYGGLPSDRLFVTWPLRSERVRSRLLGRYEPLAPGALATLPEYAPG
                     TSESRCRLAFPGDFDGLLASDPAQAREWRFRLRDNLEAAFASGYVITGFAGRRGEPHG
                     YYMLERDFVFE"
     gene            7081..8202
                     /locus_tag="VHM69_06450"
     CDS             7081..8202
                     /locus_tag="VHM69_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX2740068.1"
                     /translation="MRITGVEIIPVKLPLLEPFVVSYGTFPDLATVLVRLETDGGLIG
                     WGEGTPDPHVTGETFEGVVATLRHLAPVLLGRDPLDRSTAMRLLGSRVAGAPTAKAAL
                     DIALHDLAGHVASLPVWALLGGRARETLSISRVISLKSPEAMADDATRHVAAGFRTVK
                     LKVGDAGNIRGDVRRVAAVREAVGPDIGIKIDVNGGWRTAGAAVRAARGIAPYDPEYV
                     EQPVDRRDLEGMAEVRRLSGVPVMADESVLDARHALRAVRLRACDLINIKLMKCGGLL
                     AALTLDAVAETAGVGCQIGTMVESSVASAAGLHLALALHNAATVEMGGPIMLAEDVSD
                     LRAYYERDRVTVPDGPGLGVEPDETVLQRYAGERDWITA"
     gene            8612..9043
                     /locus_tag="VHM69_06455"
     CDS             8612..9043
                     /locus_tag="VHM69_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740069.1"
                     /translation="MTTRKPDLPVIPFVSRDAWEEWLEEHQATSDGLWLRIAKKGSGI
                     ESVTFADALEVALCYGWIDSQANSFDEQYWLQRFTPRRPRSKWSMRNRARATKLIEEG
                     KMKYAGQREVERARADGRWEAAYLPHNSTATVKVYGGRRAK"
     gene            complement(9265..>10444)
                     /locus_tag="VHM69_06460"
     CDS             complement(9265..>10444)
                     /locus_tag="VHM69_06460"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX2740070.1"
                     /translation="VVGIISGTSCDAIETAAAELHLESDTLVMRPLGARGFDYDVELR
                     EAVMATLPPSATSVEDVVKLDTRLGQAFADAAAGAVDEFCGGRADLVVSHGQTVFHWV
                     EGSRALGTLQLGQPAWISARTGLPVVSDLRSRDIAAGGHGAPLVSLFDILLLGESPKT
                     RAALNLGGIANITVVSEKRPPLAYDTGPANALIDAAVQRFSGGSEHFDKGGLRGARGN
                     VDERLLERLLADPYYDALPPKSTGKEHFHLPYLLDKLDESSPLDEDDVIATLTALTTR
                     TIAREIERWQIEEVVAAGGGTANPTLMGMLAQAAPEVELRTTEAWGIPPGSKEAYAFA
                     LMGFLTMHGLPANVPSCTGADSPVVLGSITPGGSPLVMPDPAPGAPGRLRIEAAVPGI
                     "
BASE COUNT         1995 a         3299 c         3334 g         1816 t
ORIGIN      
        1 gcataccacg agggcggtca cgccatcctg ggcctcctcg tgccgggggc ggaccccgtc
       61 aaccgggtga cgatcgtacc gcgcggtcag gcacttggcg taacctacca gcgccccgag
      121 gacgaccgct acaactaccc ggaagcatac ctgagggcga ggatagtcgg cgccctggga
      181 ggacggagcg cggaggagat cgtctacggc accaagacca caggggccga aagcgacatc
      241 gagcaggcca ccaacctcgc acgcaggatg gtcacccgct ggggaatgag cgatgagctc
      301 gggacggtag aactcgcgcc ccgcgacaac ccttacctgg gtggatctgg cggttacggc
      361 ggggagagga ccttcagcga ggagaccgcc cgcatcgtgg actccgaggt gctcaggatc
      421 atcaacgagt gccacaacga ggcgcggcga ctcctgaccg agcaccgcaa ggagctcgag
      481 gcgctcgctg cggccctgct ggagcgggag accctcgacg aggaggagat cctggaggtc
      541 acgggactcc cgcccgcgcc gccgctggag aacaggaagg tcccgatacc cgacgccgac
      601 tcccatgcat aggcgcgaga cacggcttga gccgcaccac ggccctttat agggagaacc
      661 ccatgaccgc cgaaaatacc gccaacccgg ccgccctcaa caccatcgtc ctgatccacg
      721 ggctgtggat gacgcccttg agctgggagc attggataga cctctacgag gcgcgcggct
      781 tccgagtgct cgcccccgca tggcccggga tgggcggcga catcgatgag ctgcgccgcg
      841 atccgtcggg catcgagcac ctgggtatca gggagatcgt cgaccactac gccgccatca
      901 tccgcgaact cgtctgcccg ccgatcatca tgggccactc ctttggcggt gcgttcacgc
      961 agatcctgct tgaccgtggt cttggcgccg cgggggtggc gatcgactcc gctgccgtca
     1021 agggcgtcct gacgttgccg ccgtggtcga cattgaagtc cgggttcccg gtgctcaaga
     1081 gccccgccaa caaccatagg gcggtcgcgc tgagccttga ggagttccgg tatgcgttca
     1141 cgaacacgct aagcgaggag gagtcacagg cggcctacga gcgctacgcc gtccctggcg
     1201 ggtactcttc caggctgcac tggcgacctt caacccacgc gccgcaacta atgtcgactt
     1261 caacaacgac gaccgcgcac ctctgctgtt cattgcagga gggaaggatc acgtcagtcc
     1321 cgccgcgatc aacgagtcca acgtcagacg ttatctcaag tccaaggcgg tcacggtgta
     1381 cgaggagttc cctctccgcg ctcatttcac gctgggcgag gagggctggg agcaggtcga
     1441 cgccctcacc tggaccatcg agaacgctgc tgtgcaccag ctgcccgcga ccacctaatc
     1501 ggcctcacct tggctacacc tctacttccg cccttactcg ccggaattcg cgccggcggg
     1561 taacaggatg ctctgtgaag atgcggtttc ggacatacga tcggcgccac cgtatatagc
     1621 ggcgagagga gatacttgtt ttggaccttc gaatggcagg aaggtcgttg tcgtcaccgg
     1681 agcaagggca ttggcttggc catgaccagt gccctgatta gcgagggtgc tagcgtggtc
     1741 gtcgcttcgc gaaacctcac caacgagttg tcacagctca tcagccaatg cgcggtctac
     1801 ctctgggcac ctcggacgga ccagcatggc tgataggcga gttcatcggg tcggatatcc
     1861 tgatcaacaa tgcgggcgtg atccgtccgc gtcccggcgg gttcctctcc ctcaccgacg
     1921 aggactggat gtggggactg aacatgagct tcctggtcgc tgttcgcacc acttgcgctg
     1981 cgcttcaaca cctgctcgac cacggcgccg gcaccaacgt caacacgtgc tcggtccacg
     2041 ccgtgcagcc aaagccgccg gtaatcgaca cgtaacagaa cccgcgactt tgtgttcctg
     2101 ggcaccaatg cccactccgt ctgtgtgtcc ggtagggaaa tgaacaacga aaagaccgtg
     2161 cgccgcgtgt acgaggattt gctgggttac ggcgaggtga agcccgacct acttcaggag
     2221 cgctacggcg cgtacgaccc aagacccgtg gtgtcttcga ggatctgctt ggcttcctcc
     2281 accaacgacc caggcactag cacgtccctg gggccggctg ccaggaagtc cggcgcatcg
     2341 aagccggctg cacgttggat cagcgaaggg atgccgacct cctttagcac accctccatc
     2401 agcagcgcaa cagattcgtc cggggccgca gctactttca cccatctctc ctccatgcgc
     2461 ggattctacc ctaatggcta tccaccttgc ctataagaag tcaggtccga agtgtgcatc
     2521 cagaatcctc tatgaaccgc ctgtggcgag atgtggtagc gactgttcgg tagatttgac
     2581 gggaatattg acaccactct gacaccaccg ggaacgcaat tccgtgcaac acacagccag
     2641 ccgaagaaag gaaaatccct tagatatgcg gcaattgcag gcccgtgcaa gcccctgcgc
     2701 cacctgaatt atcactcgta atgagatgag gtcagcggtt cgagcccgtt tgccccgatt
     2761 cgttagccgc atatcgagga tctctcgtgt ggaagcatgg cacgggattg tgagatgcga
     2821 tcaaagtgag gcgtccgctt ccacggggct tacaggctcg attctcgtcg agtttgctct
     2881 ccccggccgc cctcgagatc tcaggtaaga ggcgcattca cgtcgacgac tgccccggtc
     2941 atgaaatccg gagcatccga tgccaggaac gccaccatac gcacgacttc ctcgaccccg
     3001 ccttaacggg atcgtccgcc gtagcttcgt taagagccgc cgatgccgga tcatccatgc
     3061 actgaagagc attttattca acacctcaag atgttgtggt cggcaccgcc tcgcgaatcg
     3121 gcgggaaccg ggtctgtgcg ccactcggga ggctttctat gcaggaactt ggatttgaac
     3181 ttcgcagaag gggtgagagg cgaagctcgt tgggtgagct cgctgtctga ctcgcgatgc
     3241 ggtcggggga acagaatacg gtcgtgctga tctggtgttc tcttgcaagc aatacttatc
     3301 cccgagtcag gcatgaggcg cgtcctcggc gatgtgcacg tccgcgccgc ggggaatcac
     3361 gacgaccggc accggagaac tacgcacgat cttggtcgag ttcgagccga ggaataccct
     3421 cgccacaggc ccgaggcggc tcgatcccac cacgagcacc tcaccctcct cccacgggag
     3481 cgagtccagc gcgtcgtccc agtcgcgacc ttcgacgatg ccggcctcca cctccgtgac
     3541 gttgtccggc agtgtggcca gcgccttctc ctgcgcctcc agggcctgcg cgcgccactg
     3601 ctcggcgacg aggcgctctg catcgtagcc tacctgcgag ggatacattt gccgatcgcg
     3661 gactacgaag gtggtaagct gcagcggcac gccgtgtcgg ctgctcagtt gtaaagcagc
     3721 attgaacgcg ctcctcgatt gcatggaccc ggagaacgcg catgtgacgc ggcgtagccg
     3781 cgtgtcggaa ggcagccggt atccccgggg ggcgagcgcc acggggaccg gagatacgtg
     3841 tagcatctcg ttggcgatgc cgccgaccgt gatccgcccc aaaggcccgt atcgtgcgga
     3901 gcccaaagcg attagcgccg cgcccttctc cgtggcagtg gcgataagcc cttcggacgc
     3961 cgacgatgcg gagatggaag tgtactctgc ccgtacctca tccccgagga actcgcgcgc
     4021 ctcagcaatt gcatcttcag cgtactgctt caggaacgcg gcgtactccg cgtccacgcg
     4081 cgccggcgat gggtagtccc aggtctgcgg cacgacaacg cacacggtga gggtgacgtc
     4141 gccggcgcgg gccagcatcc ggccgagact gagcgcgtct cggccgccca gagcagtcaa
     4201 cgcgacgagg tagttcatcg cggctcttcc tccgtggcga cgtcgatccc cctcgccagc
     4261 cgcgagtgcc tggcgctgta ggtgaagtag atgatcgccg cgaccccgag ccacaccgcg
     4321 aacagcgcga acgtcgagta aggcagaccg gagatcaggt atagggcgaa ggcagcgctg
     4381 agtatgggcg ttaccgggta gccgggaacc ctgaacccgc ggggcaaatc cggctgggtg
     4441 cggcgcagga ttatcacccc gatcgagacc accgtgaacg cgaccagggt tcccatgctc
     4501 gtcaggtcta tgagcacgcc gagcggcacg agcccggcaa gcagcccgac gaaggcgcag
     4561 acgatgatgg tgttgcgcac cggggcgagg gtgcgcgggt ccacccggtg gaagacctcg
     4621 gggagcatcc cgtcgcgacc catggcgaac agaatgcgcg actgcccgta caagacgacc
     4681 agcgtaacgc tgaagatcga gatggtcgcg ccaagggcca acacggcggc cggccagccc
     4741 gacccggtga tgttgcgcat gatcaccgag aggccggcct cctggcctgc gaactgctgg
     4801 tagggttgtg cgcccacccc ggcaagcgcc acgagaatgt agaacgtggt cacgatgatg
     4861 gtcgcaaaga tgatcgccag cgggaggttc cggcgggggt tgtgaacctc ctcgcccgcg
     4921 gtcgagacgg cgtccaaccc gatgaaggag aagaagatcg acccggcggc cgcaccgatg
     4981 cccgcgatcc cgaagggcgc gaacggctcg aggttctccg ccgagaaggc cgtgaatgcg
     5041 acgacgatga acagggcgag aacggcgagc ttgatgagca ccatgatcgt gttcgccgtg
     5101 gcggactccc gcaccccacg gatgagcagc aaaccgcaca gcgtcacgag cacgacggca
     5161 ggcaggttga tgaacccgcc ctcccccggt gccgcgttga tggcgcccgg aatgcgaaag
     5221 ccgaacacgt cgaagagcaa ctcgttgagg tactgtcccc accccacgga cacggcggcc
     5281 gcggagaccc cgtattcgag gagcaagcaa gccgccacgc cgaacgccac gatctctccc
     5341 atcgtcgcgt aggtgtagga gtaggacgag ccggagacgg gaatggtcga ggcgagctcc
     5401 gcgtagcaca ccgcggtcag ggcagccgtg atcccggcga gggcgaagga cacaatgacc
     5461 gctggaccag cctccggcac cgcctctgtg agcacgatga agatgccggt gccgatcgtg
     5521 gccccgaccc cgaacatagt aagctgaaac aatccgatcg agcggctgag ctcgccgccc
     5581 tcggcgtcgg ggccggtctc ggtgacgaac gcctcgaccg gcttcctccg gaacaggctc
     5641 cctcgtcgca tgcgcgtccc tgacatcttt gccccttcca ctcaaccccg agaatggtat
     5701 tgtaacctat gtgccaggat gtaggtggag cagatgacca tgggaccggg ttaggggacc
     5761 acgcgacagc cggatacaat agacgcatga gtatcgaaga ccttctgctg gaggtgacgc
     5821 gtttgtacag gaccaacggt ctcaccggta ttcaaccctg cgacgaatat ggtatgagcg
     5881 gaaccggctc cagagccggc agtccggata gtttgctgtt tgcacacgct cacccggacg
     5941 gtggtcgaga ggttttgacg taggtacgca cggatatggc atacgaggac agacgtggat
     6001 tgcaaccacg tgggaggacg cgtcgaggat ggctcggtac cctatgttga cacgatctcg
     6061 tcagcttgcc ccgcgcaaac gttcgccaat ctagaagtcg ctttccgttg acgctaagta
     6121 tctcttacca gtgtttcgag aggcaacatt gagtgcgccg tctttggcag atccggttgc
     6181 tctccagacc ctgggtttcg agagctgctc gaacggttgg tggaagacca ttggtgagcg
     6241 gcgtctgcgc ttcacgcgcc tcatctcgca cctggacctg gagaaaaccg aacggcttca
     6301 gtacggggtc ttcggggtgt ctgaccgtga cctggcttca tcgagcatcc tcgttaccgt
     6361 acacgaaacc ggcggggagg tcctcggggc tttcgatgga gaacagctgg tgggcttcgt
     6421 atcgggttgg ggcggttacg tggacggccg ggcgcgcctt gtctccgacc tgatggcagt
     6481 cgaggccgga taccggggcg gtatcgggtt caccctgaaa gcgctccagg ccgtcgtcgc
     6541 ttcccaggct ggtttcgagg agatcgtgtg gaccgtcgat ccactgcggg ccgcgaacgc
     6601 acggctgaac tttgagcgtc tcggcgccca ctcgcgcaaa tacatgcgta atgtgtacgg
     6661 ggagcatttc ggagaaggcc tctacggggg cctgccaagc gaccgtctgt tcgtcacctg
     6721 gccgctccgt agcgagcgtg tgaggtcgag gctgctggga aggtacgaac cgctcgcgcc
     6781 cggcgccctc gccacgctac cggaatatgc tcctggcaca tccgagagcc ggtgccggct
     6841 cgcctttccc ggcgacttcg acggtctcct cgcctccgat ccagcccaag cgcgcgagtg
     6901 gcgctttcgg ctgagggata acctcgaggc agcctttgcg tccggatatg tcatcacggg
     6961 tttcgctggt cggcgcggcg agccccatgg ttactatatg ctcgaacgcg atttcgtgtt
     7021 cgaatgagac cgagcacctc tacctgattc tctgcagtcg aagatattcg aggaggcaaa
     7081 gtgcgaatca caggcgtcga gatcataccc gtcaagctgc ccctcctcga gccgtttgtc
     7141 gtctcttacg gaacgtttcc ggacctggcg accgtgttgg tgcgtttgga gacggacggg
     7201 gggctgatcg gttggggaga ggggacaccc gacccgcatg tgaccggcga gaccttcgag
     7261 ggagtcgtcg ccacgctgcg ccacctcgcc ccggtgttgc ttgggcgaga cccgctagac
     7321 cgttccaccg ccatgcggct gctgggatcg cgggtcgcgg gcgcgccgac ggcgaaggcc
     7381 gcgctcgaca tcgcgctgca cgacctcgcc gggcacgtcg ccagcctgcc ggtctgggcg
     7441 ttgctgggcg ggcgcgccag ggaaaccttg tccatctccc gggtcataag cctgaagtcg
     7501 cccgaggcga tggccgacga tgctaccagg cacgtcgcgg caggctttcg cacggtcaag
     7561 ctcaaggttg gggatgccgg caacatccgg ggcgacgtgc ggcgggttgc ggccgtgcgc
     7621 gaggcggtcg ggccggacat cgggatcaag atagatgtca atggagggtg gcgtacagcg
     7681 ggcgcagcag tccgcgccgc gaggggcatt gcaccctacg atcccgagta tgtcgagcag
     7741 ccggtcgacc gacgcgacct cgaaggtatg gctgaggtgc gtcgtttgag cggcgtgccc
     7801 gtgatggccg acgaaagtgt actggacgcc cgccacgctc tgcgagcggt tcgattgcgg
     7861 gcttgcgacc tgatcaacat caagctgatg aagtgtggag ggttgcttgc cgcgctgacg
     7921 ctcgacgccg tcgccgagac cgctggagtc ggctgccaga tagggaccat ggtcgaatct
     7981 tcagtggcat cggcggccgg cctgcacctc gcccttgcgc tccacaacgc cgcgacagtg
     8041 gagatgggtg gaccgatcat gctggccgag gacgtcagtg acctgcgcgc ctactacgaa
     8101 cgcgaccggg tcaccgtacc cgacggtccc ggcctcggtg tcgagccgga tgagaccgtg
     8161 ctgcagcgct acgccggcga acgtgattgg atcactgctt gaccccaacc gcgcttcctg
     8221 gtgatgctcg tatgcatcca ggtgcggtgc cgcgggaagc cgcgtgcggt tttgtggggg
     8281 cttcgtacga ttcggcgcac cggcttcggt gatggaagtt cgacgtgctt gccgaccccg
     8341 agcgtctgag acaccttgac gactgacgat cggccacgct gcgcgattcc attgccccta
     8401 cactcatgtc ggaaacttcg cgatgcacca tgcggtctcg gttgacggca aaagggccac
     8461 cgatgtacgg attccgcttg ggagtccgcg gcaagtcccg tagactttca gcatagtcct
     8521 tccaacggaa ggacgggaaa ggccgctcca ccgcgcgctt ccccgatcct tctcggcgcg
     8581 cagcggcaca tgtgagtgaa aaggagacgc cgtgaccacc aggaagcccg acctgccggt
     8641 aattccgttt gtttcacgcg acgcgtggga ggagtggctt gaagaacacc aagcgacctc
     8701 ggacgggttg tggctcagga tcgccaagaa gggttccggc atcgagagcg tcaccttcgc
     8761 agatgccctc gaagtcgccc tctgctacgg ttggatcgac agccaggcga acagcttcga
     8821 cgagcaatac tggctgcaac ggttcacccc gcgccgtccg aggagcaagt ggtccatgcg
     8881 caaccgcgcc agggcaacga agctgatcga ggaaggcaaa atgaagtacg ccggccaacg
     8941 agaggtggag cgtgccagag ccgacggccg ctgggaggca gcctacttgc cccacaatag
     9001 caccgcgact gtgaaggttt acggcggaag aagagcgaag tgacaatgga gttttcgtca
     9061 cgcgctgcac gtctgggccg aaggagatga cgtgccagtc cttccgccga gccaatcacg
     9121 tcacgccgtc atccgacaag acgacggaga gactctcgaa atgatggatc tcgcattgcg
     9181 tcgaaaaagc tgaagtgctg agaagctgcc tcaggcagcg tgctgacgag cgcggaatgc
     9241 agaaatgatc attgtgccat ccgtttagat gccggggacg gcggcttcga tccgcagacg
     9301 accgggtgcc ccgggtgccg gatccggcat taccaggggc gagccgcctg gtgtgatgct
     9361 gccgagcacc acaggcgagt ccgctccggt gcaagagggc acgttcgcgg gcaggccgtg
     9421 catcgtcagg aagcccatga gggcgaaggc gtatgcctcc ttgctgccag gcggtatgcc
     9481 ccatgcctcc gtcgtgcgga gctccacctc cggtgccgct tgtgcgagca tgcccatcaa
     9541 agtcgggttg gccgtgcctc ctccggcggc cacgacctcc tctatctgcc agcgctctat
     9601 ctccctcgct atcgtacgcg tggtgagcgc cgtcagggta gcgatgacat cgtcctcatc
     9661 caggggactg gactcgtcga gcttgtctag caagtacggc aaatggaagt gctccttgcc
     9721 cgtcgatttc ggcggaagcg catcatagta agggtcagca agcaatcgct ccagtagacg
     9781 ctcatcgacg ttgccgcgcg cgccccgcag tccaccctta tcgaagtgct cggaaccgcc
     9841 ggagaagcgt tgcaccgccg cgtcaattaa agcgttggca gggccggtgt cgtaagccag
     9901 cggtggacgc ttctcggaaa cgaccgtgat gttagcgatc ccgcccaggt tcagcgctgc
     9961 gcgcgtcttc gggctctcgc ccagcaacag gatatcgaag aggctcacga gcggcgcacc
    10021 gtgtccgccg gcggctatgt ctctgctccg caggtccgac accaccggca atccggtgcg
    10081 ggcggagatc caggccggtt gcccgagttg cagcgtcccg agggccctgc tcccctctac
    10141 ccaatggaac acggtctgcc cgtgcgaaac gacgaggtcc gccctcccgc cgcagaactc
    10201 atccacggcc ccggcagcag cgtcggcgaa cgcctgtccc agacgtgtat ccaacttgac
    10261 cacgtcttcc acactggtgg cggagggtgg aagggtagcc atcaccgcct ccctcagctc
    10321 cacgtcgtag tcgaaaccgc gcgcgccgag cggccgcata acgagggtgt cgctttcgag
    10381 atgcaactcc gccgccgcgg tctcgatggc atcgcatgac gtccctgata tgatgcccac
    10441 tact
//
LOCUS       DATAIR010000265         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_53884, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000265 DATAIR010000000
VERSION     DATAIR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53884"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VHM69_06465"
     CDS             <1..758
                     /locus_tag="VHM69_06465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2740071.1"
                     /translation="LPALTTRPSRKKRRKATDEKPGEIAAIFDVDGTLVGSNVVSYFA
                     WLRMRELPPALRPLWLAGFLTKIPYYWGLDKLSRAHFNRAFYKNYAGWEPARARHLGQ
                     ESFAGFTLDRIFPDALRQLRDHKAAGHRVVLLSGALDFLLEPMKDLVDDVLCATLAEE
                     NGAYTGELTGAPVAGDARARMLASFARRRGLDLSRSYAYADSISDLPMLEAVGNPVAV
                     NPDRRLGAAAKDRGWRVEHWDKNGVPNGVAKKI"
     gene            760..1995
                     /locus_tag="VHM69_06470"
     CDS             760..1995
                     /locus_tag="VHM69_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2740072.1"
                     /translation="MQSLVYRKSVPRYLLMRAVSKRIKKLDTSRFSPLQLEDIPNPEL
                     PTPEWVRVKPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEVVGEVVEDNSGFST
                     GERVVLEPALGCRVRGIDPPCPYCASGRQALCLNVAKGDIGPGIQTGFCHDTGGGWSQ
                     RTLVAHPSQLHRVPEDVPDEAAVAIEPFACAVHAALKAAPSPDDTSLVIGTGSVGLLT
                     VAALRRLTDAGRIICVAKHERQKTEALRLGADEIVSPKEIYTGLPELLGTDVYRPELG
                     KPVVMGGADKVFECVGAPGTMEDAVRLTKPGGEATLVGMPGAKSSLDLTALWYKEVRF
                     TGSYAYGIEEYEGENTSSFALALRTANEIRLDTLVGPRFHLEEYREAIAAARSAGREG
                     HVKVVFDHRGHRLQASGVR"
     gene            2153..>3702
                     /locus_tag="VHM69_06475"
     CDS             2153..>3702
                     /locus_tag="VHM69_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX2740073.1"
                     /translation="MSRPGFISVLEKNSPPMMFNAGDGFHYEKLPEGTRVIYPPGPQD
                     PLADPNVAIERALLEPLDMEPLHDLLFPGMKLTIAFDDLSLPLPPMQRPDARQLVIEK
                     VLEKALAKGVEDIHIIAALGLHRRMTEGELRHCLGKKIMTQYYPDRLYNYDAEDEENH
                     VTIGETEKGEEVTINRRVAEGDLLVYVNVNYIPMDGGHKSVHTGLSPYSSIRHHHTPD
                     TLSHTRSLMDPPNSAMHASINRMGRLLDENVKVFHIETTLNNAAFPAFADFMAKPEHQ
                     WSPVTKANFLAMHNATQRAPRRISRRIFHTIKGPHRMTSVHAGATDPVHAETLKNNYE
                     QQVVPVEGQADVLLLGIPYLGPYNVNSIMNPLLVYNMLLGYLFNLYRGKPLVREGGVL
                     IGTHPMPEDFNKIHHPSYIDLWNEAFTETHDIREIHERYEKKYAYDPWYRTLYRSSYA
                     YHGAHPFTVLYWAAHALDHLGGAILVGGDPATTKRMGLGRADTVVEALQMAQDTVGPN
                     PSTTYMHIP"
BASE COUNT          749 a         1272 c         1087 g          594 t
ORIGIN      
        1 acctgcccgc gctcaccaca aggccgagcc gcaagaagcg aaggaaggcg acggacgaaa
       61 aaccggggga gatcgcagcg atcttcgacg ttgacgggac tctcgtcggc tccaacgtcg
      121 tctcgtactt cgcgtggctc aggatgcggg agcttcctcc ggccttgcgc ccgctctggc
      181 tcgcggggtt cctcaccaag ataccgtact actgggggct tgataagctc agccgcgccc
      241 acttcaaccg cgccttctac aagaactacg cgggctggga acccgcgcgc gcccgtcacc
      301 tcgggcagga gagcttcgcc ggttttacac tcgaccgcat ctttcccgac gccttgcggc
      361 agctacggga ccacaaggcc gccggacacc gcgtcgtcct gctctcggga gccctcgact
      421 ttctcctcga accgatgaag gaccttgtag acgacgtgct atgcgccacg ctcgccgaag
      481 agaacggcgc ctacaccggc gagctcacgg gtgcccccgt ggcaggcgac gcacgcgcca
      541 ggatgctcgc gtccttcgcc aggcgccgcg gcctcgacct atcgcgatcc tacgcttacg
      601 cggactcgat ctccgacctc ccgatgctcg aagccgttgg aaatcccgtc gccgtcaacc
      661 ccgaccggcg gctcggcgcg gcggcgaaag acaggggatg gagggtcgag cactgggaca
      721 agaacggcgt ccccaacggc gtggccaaga agatctaatt tgcaatccct cgtctaccgg
      781 aaatccgtcc cgcgctacct tctgatgcgc gccgtctcga agcggatcaa gaagctagat
      841 acgagtcgct tctccccgct gcaactcgaa gatattccga acccggagct cccgaccccc
      901 gagtgggtgc gcgtcaagcc gctcctctcc ggtatttgcg gctcggacct ggggacactc
      961 tcctccgaga actcgccgta tttctccccg atcacctccc cacccttcgt gatgggtcac
     1021 gaggtcgtcg gtgaggtagt cgaggacaac tcaggcttca gcaccggcga gcgggtcgtc
     1081 ctcgaacctg ccctcggatg ccgcgtgcgc ggcatagacc cgccttgccc gtactgtgcc
     1141 tcgggccgcc aagctctgtg cctgaacgtg gcgaagggtg acataggtcc gggcatccag
     1201 acgggtttct gccacgacac aggaggcggg tggtcgcaac ggacgctcgt cgcccaccct
     1261 tcgcaactgc atcgcgtgcc cgaggacgtc cctgacgagg ccgcggtcgc catcgagccc
     1321 ttcgcctgcg ccgtccacgc cgcgctgaag gcggctccat caccagacga tacgagcctg
     1381 gtgatcggga ccggcagcgt cggcctcctg accgtcgccg ccctccgccg actcacggac
     1441 gccggacgca tcatctgtgt cgccaagcac gaacgccaga aaaccgaggc ccttcgtctg
     1501 ggcgcggacg agatcgtcag tccgaaagag atctacacgg gactccccga gctgctcggc
     1561 acggacgtct acaggcccga gcttggcaag cccgtcgtca tgggcggggc agataaggtc
     1621 ttcgagtgtg tcggcgcgcc aggcaccatg gaagacgctg tacggctcac gaagcccggc
     1681 ggcgaggcaa cgctcgtcgg gatgcccggg gcgaagagca gcctggatct gacggcactc
     1741 tggtacaagg aggtccgttt cacaggaagc tacgcctacg gcatcgagga gtacgaggga
     1801 gagaacacga gcagcttcgc actcgcgctg cgcaccgcaa atgagatacg actcgatacg
     1861 ctcgtcggcc cgcgctttca cctcgaggag tacagggagg ccattgcggc cgcgcgttca
     1921 gcgggtcgcg aagggcacgt caaagtagtc ttcgaccaca gaggccacag gctacaggct
     1981 tcaggtgtca ggtaaggtct aagcgctgcc gcatgggagg aaaccgaaca tgtgtcctgg
     2041 agaacgggtg ccactaaggg tacgttcctc ggccgtggca atcctggcca ccagcccctg
     2101 aagcctgtag cctgatgcct gaaacctaag agcgaccgaa gggagcgacg acatgagccg
     2161 acccggattc attagcgtat tggagaagaa cagcccgccc atgatgttca acgcggggga
     2221 cgggttccac tacgagaagt tgcctgaggg gacgcgtgtg atctaccccc caggacccca
     2281 ggaccctctg gcggacccga acgtagccat cgagcgggcg ctgctcgaac ccctggacat
     2341 ggagccgttg cacgatctgt tgttccctgg catgaagctg acgatcgcct tcgacgacct
     2401 ctcgttgccg ctcccgccga tgcagcggcc cgacgcgagg cagctcgtca tcgagaaggt
     2461 gcttgagaag gccctcgcga agggcgtcga ggacatacac atcatcgcgg cgctcgggtt
     2521 gcaccgcagg atgaccgaag gcgagctgcg gcattgcctg ggcaagaaga tcatgaccca
     2581 gtattacccc gaccgcctct ataactacga cgccgaggac gaggagaacc acgttaccat
     2641 cggcgagacc gaaaagggcg aagaggttac gatcaaccgc cgcgtcgcag aaggcgacct
     2701 gctcgtctac gtgaacgtga actacatccc tatggacggc ggccacaagt ccgtccacac
     2761 gggtctctcg ccttattcct ctattcgcca ccatcacacc cccgacacgc tctcgcacac
     2821 ccgctccctg atggacccgc ccaactcggc gatgcacgcc tccataaaca ggatgggcag
     2881 gctgctcgac gagaacgtca aggtcttcca catcgagacg accctgaaca acgcggcctt
     2941 ccccgctttc gccgacttca tggcgaagcc ggagcaccag tggagtcctg tgacgaaggc
     3001 gaattttctc gctatgcaca acgcgacgca aagagctccg cgaaggatct cgcgccggat
     3061 cttccacacc ataaagggcc cgcacaggat gacgagcgta catgccgggg caacggaccc
     3121 cgtccacgca gagactctga agaacaacta cgagcagcag gtggttcccg tcgagggcca
     3181 ggccgacgtg ctgctcctcg gcatcccgta cctcggacct tataacgtca actcgatcat
     3241 gaaccccctc ctcgtttaca acatgctcct cggctacctc ttcaacctct acaggggcaa
     3301 gccgctcgtg cgcgagggcg gagtcctcat cggtacccac cccatgcccg aggacttcaa
     3361 caagatccat caccccagct acatcgacct ctggaacgag gcgttcacgg agacgcacga
     3421 tatacgcgag atccacgagc gctacgagaa gaagtacgcc tacgaccctt ggtaccgtac
     3481 cctctaccgc agctcctacg cctaccacgg cgcccacccc tttacggtcc tctactgggc
     3541 cgcccacgcc ctcgaccacc tcggcggcgc catactcgta ggcggcgacc ccgcgaccac
     3601 gaagcgcatg gggctcggac gggccgacac cgtagtcgag gctctccaga tggcgcagga
     3661 caccgtcggc ccgaatccaa gcacgaccta catgcacatc cc
//
LOCUS       DATAIR010000266         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_109576, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000266 DATAIR010000000
VERSION     DATAIR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109576"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1121)
                     /locus_tag="VHM69_06480"
     CDS             complement(75..1121)
                     /locus_tag="VHM69_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HEX2740074.1"
                     /translation="MNRYRPQRNWGARVIEYRYRGMRAVFLENEILRVGVLADKGTDV
                     FEFNYKPCDMDFVWLTAGGVRNPTSYLSTSPDPLATFMDYYQGGWQEIFPNGGAPSSY
                     LGARFGQHGELSNLPWDYEIVEDDEDSVAVRFSVRTQKTPFYLEKELRLRSGEGRLLV
                     EETLTNESETPLHAMWGHHLAFGRPFLDEGCRIRLPGGATVMPHDVPTHPKGRRIKGD
                     KHYVWPLAEGESGEEVDLSVLPQRGTLPEMLYLTDLSDGLYEIENPEKGLGLRVGWDV
                     GQMPYLWFWQEYGASDFYPWYGRHYNIGLEPFSSFPTNGLEEAVDNGTAISLGAREMR
                     RFSLQAEVFESGPD"
     gene            complement(1160..>3363)
                     /locus_tag="VHM69_06485"
     CDS             complement(1160..>3363)
                     /locus_tag="VHM69_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX2740075.1"
                     /translation="RSAADRCDEYPEFVFTRGESWLFEQVERIDPGLFERVRRQVESG
                     QWHITGGQYLQPDVNLSTVMGLHRQIMHGQRYFKDRFGVSPKVGYNVASFGHPATMPD
                     VLASHGYVGYVFHRPGAHQVELPAQTFRWRGANGGEVIGFRISPAYVTFSDDLYGQIM
                     LSIEAANPGLGHTMCFYGVGNHGGGPTKANIEYILENVHSFPDAELRFSTPQAFFDAI
                     AESRDRLPVVTKELQHTFPGCYSVMHDIKQQQQRGEHLLDQCGRLVDGLVYDEVERRE
                     LNGRLDAAWDDLLFTEFHDVLAGTSIPAAWDSIRAMQGRARIIGEEVAVEATRRWAVR
                     KLPPIDDQQIVVINPDDASWEGMVQTEPFISFDAWGKRWLSDLEGRPIDFQVVQPDAA
                     AHVERVIFPLRLDARGTSQILVRTDERPPTEAPDTDLEASSQLIANSHLRAELDGSGI
                     RRLISEGRDLLGEDGVRLHLRRDHTDTWTFHTDRFVEPVETVLHTDGWVVEESGPLRA
                     RVRSEGWLGRSAVRWTLTLHRDDPRIHISLEVNFNERLKLLQMPFHLAASPARRTDGL
                     PGGHVERTAGPTEWPVQGWSRVELEDRQLALVTGDAYSLSLDGDRWQWTLLRSPKMAW
                     GGGEPEVYAGRDTYTDQGPHTFDFVLYTGGQLDETMLHTAARQQMQPPVVFDRYEGMD
                     RPPWGNSPPRALWLGAVERALADGRMGHVLDNQDAHAVRPLFERVERDQGE"
BASE COUNT          574 a         1106 c         1033 g          650 t
ORIGIN      
        1 cgccctatac ccagagcccc accggcgacg atcgccactt tcccggagaa ttccgccacc
       61 ttcttcctcc tctcctagtc tggtccactt tcgaacacct cagcctggag tgagaaacga
      121 cgcatctccc tggcgcccag agagatagcc gtgccgttgt ccaccgcctc ttcgaggccg
      181 ttcgtcggga agctggagaa gggctccagc ccgatattgt agtggcggcc gtaccacggg
      241 tagaagtccg aggcaccgta ctcctgccag aaccagaggt aaggcatctg tcccacgtcc
      301 catcccaccc tgaggccgag acccttctcg gggttctcga tctcatataa gccatcggag
      361 aggtccgtga ggtagagcat ctcagggagg gtcccacgct gcgggagcac gctcaggtcg
      421 acctcttcgc cggattctcc ctctgcgagg ggccacacat agtgcttgtc tcctttgatc
      481 cggcgtcctt tgggatgcgt cggcacgtcg tgaggcatga ccgtcgcccc tcctggaagg
      541 cgtatccggc agccctcgtc gaggaaggga cgaccgaagg cgaggtggtg gccccacatg
      601 gcgtgcaaag gcgtctcgct ctcgttcgtg agcgtctcct ccacgagcag acggccctct
      661 ccggaccgga ggcgcagctc cttctccaga tagaacggcg tcttctgggt cctgacggag
      721 aacctgacgg ctacggagtc ttcgtcgtcc tcgacgatct cgtagtccca cgggaggttg
      781 ctgagctcgc catgctggcc gaacctggct ccgaggtagg aagacggagc gcccccattt
      841 gggaagatct cctgccagcc gccctgatag tagtccatga aggtggcaag agggtcggga
      901 gaggtcgaaa gataggaggt cgggttcctc acgccacccg ccgtcagcca cacgaagtcc
      961 atgtcgcacg gcttgtagtt gaactcgaag acgtcggtgc ccttgtccgc tagcaccccg
     1021 acacgcagta tctcgttctc caggaacacc gccctcatac ccctgtagcg gtactcgatg
     1081 acgcgagccc cccagttcct ctgtggcctg tatctgttca cactcgcggc tccttgaccc
     1141 gcacctccac cgtctttctc tattccccct gatcccgctc gaccctctcg aagagaggtc
     1201 ggaccgcgtg cgcgtcttgg ttatccaaca cgtgacccat cctcccgtcg gccagggcgc
     1261 gttctaccgc gccgagccac agggcacgcg ggggactgtt cccccagggc gggcggtcca
     1321 tgccttcata acggtcgaag acgaccgggg gctgcatctg ctgccgtgcc gcggtgtgca
     1381 gcatagtctc atccagttgc ccgcccgtat agaggacgaa gtcgaaggtg tgcgggccct
     1441 ggtccgtgta cgtgtcacgt cctgcgtaca cctccggctc accgcctccc caggccatct
     1501 tcggactcct gagcaaggtc cattgccagc ggtcgccgtc caggctgagg ctgtaggcgt
     1561 cgccggtcac gagggccagt tgccggtcct ccagctccac acgggaccag ccctgcaccg
     1621 gccattccgt cgggccggcg gtgcgctcta catggccgcc gggcaacccg tcggtccggc
     1681 gggcggggga agccgcgaga tggaagggca tctgcagcaa cttcaagcgc tcgttgaagt
     1741 tgacctctag gcttatgtga atgcggggat cgtcgcggtg cagggtgagg gtccatcgca
     1801 cggcggagcg gccaagccat ccctccgagc gtactcgcgc ccgcaatgga cctgactcct
     1861 ctacgaccca tccgtcggtg tgtagaaccg tctctacggg ctcgacgaaa cggtcggtgt
     1921 ggaaggtcca ggtgtccgtg tggtccctgc gcaggtgaag acgaacacca tcctctccca
     1981 gcaggtctcg tccttcagag atcagacgcc ggatccctga tccgtccaac tccgcgcgca
     2041 ggtggctgtt cgcgatgagt tgggacgatg cttccagatc cgtgtcagga gcttcggtgg
     2101 gagggcgttc atccgttctc acgagaatct gggatgtccc cctggcgtcg aggcgcaggg
     2161 ggaagatcac gcgctccacg tgcgcggctg cgtcgggctg cacgacctga aagtctatgg
     2221 gccttccttc gaggtcactc aaccaccgct tgccccatgc gtcgaagctg atgaacggtt
     2281 ccgtctggac catgccttcc caggaggcgt cgtccgggtt gatgaccact atctgctgat
     2341 cgtcgatcgg cggcagctta cgaaccgccc agcgccgggt ggcctccacg gcgacctcct
     2401 ccccaattat cctggccctg ccctgcatcg ccctgatcga atcccacgcc gcggggatcg
     2461 aggtgcccgc gagaacgtcg tgaaactcgg tgaacaagag gtcgtcccat gcggcatcga
     2521 gccgtccgtt gagctcccgt ctctccactt cgtcgtacac caaaccgtcg acgagacgcc
     2581 cacactggtc caggaggtgc tctccgcgct gctgttgctg cttgatgtcg tgcatcacgc
     2641 tgtagcagcc tgggaaggtg tgctgcaatt ccttggtgac gacagggaga cggtctctgc
     2701 tctccgcgat ggcgtcgaag aaagcctggg gcgtgctgaa tcgtagttcc gcatcaggga
     2761 atgagtggac gttctccagg atgtattcga tgttcgcctt cgtcggtccg ccgccgtggt
     2821 tgccgacgcc gtagaagcac atcgtgtgtc cgagcccggg gtttgccgcc tcgatggaga
     2881 gcatgatctg gccgtacagg tcgtccgaga acgtgacgta ggccggggag atacggaacc
     2941 cgatcacctc ccccccattt gcgccgcgcc agcggaaagt ctgtgctggc aactccacct
     3001 ggtgggctcc gggacgatgg aagacgtatc cgacgtaacc gtggctcgcc aggacgtcag
     3061 gcatggtcgc cgggtggccg aaggaggcca cgttgtagcc gaccttcggg ctcaccccga
     3121 aacggtcctt gaaatagcgc tggccgtgca tgatctgacg gtggaggccc attaccgtgg
     3181 agaggttgac gtcgggttgg aggtactgtc ctcccgtgat gtgccactgg ccgctctcga
     3241 cctgccgccg cacccgctcg aagagcccag gatctattcg ctccacctgc tcgaatagcc
     3301 aggactcccc gcgcgtgaat acgaactcgg ggtactcgtc gcagcggtcc gcggcggaac
     3361 gga
//
LOCUS       DATAIR010000267         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_238708, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000267 DATAIR010000000
VERSION     DATAIR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238708"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..531
                     /locus_tag="VHM69_06490"
     CDS             127..531
                     /locus_tag="VHM69_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2740076.1"
                     /translation="MTTAGDKQNPLESDRGTTTIQDAVVLALASVAAQEVEHVHLSHG
                     GTRLPGDNSPTVGEFLGNVTGGMGRSRGISVEVGEQQTAVDLTLNVDYGQPIARVTET
                     VRQNVIGRVENLTGLEVTEVNITVNDVNIPEQ"
     gene            complement(1334..1810)
                     /gene="ssb"
                     /locus_tag="VHM69_06495"
     CDS             complement(1334..1810)
                     /gene="ssb"
                     /locus_tag="VHM69_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2740077.1"
                     /translation="MVSFNRVILAGNLTRDPELRFTNDGIPVCSFGLAVNRVRSRSEE
                     VDFFDISAWRELGETIANYKKKGDPILVEGRLQYRTWEAQDGSKRSKVDVVADNVQFL
                     GGRADSDDGAPSGGGQARRGGGRGGRREEVDINEEDFEDIPFRSFSIKQLIARDIL"
     gene            complement(1910..2770)
                     /locus_tag="VHM69_06500"
     CDS             complement(1910..2770)
                     /locus_tag="VHM69_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2740078.1"
                     /translation="MVALILLSPACAELELLTGGSRGGPGPPPSDSLSVSFIDVGQGD
                     GVLVQASDESYLIDAGRPEEGPNVVDFLRSRGVDALDGIVVSNPDADHIGGFLDVFDA
                     FPVETVFVSGDPNTTLTYNTFLRGVRDEGASTKVLRAGMRMDWAGVQADVIGPPTDAE
                     GGLFTEINDNSVAILLTYGTARILLAGDAEANEEEYMANGPYTGPLTLLKVTHHGSNT
                     SSTPLFLSRFPPDVAVIQCGVDNPYGHPTPETLDRLQRAGARVFRNDEDGDVIATIRD
                     QELEVAVTRP"
     gene            2992..>3250
                     /locus_tag="VHM69_06505"
     CDS             2992..>3250
                     /locus_tag="VHM69_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2740079.1"
                     /translation="MWLLSRVVAQMLFGPDSDLEVAQMSPWLDLRVPPKAVKLPMVAA
                     QTHRRFLKTHLPLDALVFSQEAKYLYIGRDGRDVVWSMYNHH"
BASE COUNT          592 a         1038 c          941 g          679 t
ORIGIN      
        1 cctccgtcga tcttgcaaca ccatgctttg ccggtgctca tcaggcgaga aggcgtgcta
       61 cgcgccgcga ggtgccagat aagatattct acggtatcac ggaaagagga cgaaagggga
      121 ggcgccgtga cgacagccgg agacaagcag aacccgcttg agagcgatcg ggggaccacg
      181 accatacaag acgcggtggt gctcgccctc gccagtgtgg cggcccagga ggtcgaacac
      241 gtccatctct cacacggggg caccaggctg cctggtgaca actcccctac cgtcggggag
      301 ttcctcggca acgtgacagg cgggatggga cgcagcaggg gcatctccgt cgaggtcggc
      361 gagcagcaga ccgccgttga cctgacgcta aacgtggact acggtcagcc gatcgccagg
      421 gtgactgaga cggtccgtca gaacgtgata gggcgcgtgg agaaccttac aggcctcgag
      481 gtcaccgaag tcaacatcac cgtcaacgac gtaaatatcc ccgagcagta gcccagtccc
      541 tggcgttgga tgacgaaccc tctctgtagg gcgtactctc gctcggccag gatgacgcca
      601 gcctgcccga gtgcgccctc cctgagccgg actcatgagg atctcatact ccggtgagag
      661 ctcacacgcc gtagcatggt cagtgttcgc agccaaaagc gacctgtagt caatggtgtt
      721 tacgaacttt ttcctgttac aggacccgca caacggctgt atgttgccga tgtcgtccga
      781 gccacctttg gtcaaaggta cgacatggtc agcctctaac gcggcttcgc cgtcaccgca
      841 agccagacat ttattgccgt acctctcgca cagttcggca aactccttgg ccgtgaaact
      901 gccgcgaccc gcgttctttg cagctctcct cttgcggcga ttgttggtct tggcttgcct
      961 gaccttctcg ggattactct cggcccactt cttgctgcga gcgattacgt cttcgcgccc
     1021 acgttcgtag taagcaagct tcgaagcagc cacgctatcc ttgttttctg ccgcccacct
     1081 cttcttgtac tcggatatct ctcgttcgtg ttcttgctgg tattttcggc gcgcctcgac
     1141 ccggtgtggc aggtttgctc tggctttctc gtatcgagcg tactgttctg agcgctcacg
     1201 acgattcttc cgtacacgca acctgacaca gaccttgcag cggttcaggt ggccgtctgc
     1261 catcctatga tgtttgtaga agcccgaaag gtctttttcg actccacagc ccctgcagac
     1321 cttcgagctc acctcacagg atatctcggg ctattaattg cttgatgcta aaggaacgaa
     1381 atggaatgtc ctcgaagtct tcttcattga tatccacctc ttctcgccgg cctccacgcc
     1441 cgccaccacg gcgggcctga ccaccgcctg atggagcgcc gtcgtcagag tcggcccgcc
     1501 cgccgaggaa ctgcacgttg tcggcgacga cgtctacctt gctccgcttg gacccgtcct
     1561 gggcctccca ggtgcggtat tgcaggcgtc cttcgacgag gataggatcg cccttcttct
     1621 tgtagttggc gatggtctcg ccgagctccc gccacgcgct gatgtcgaag aagtccactt
     1681 cttcgctgcg cgacctcacc ctgttgacag cgagcccgaa agagcagacg ggtattccgt
     1741 cgttggtgaa acgtagttcc gggtctctgg tcaggttccc agccagtatg acacgattga
     1801 agctgaccac ttctcttcct ccttctctgt accctacgag ggcccttaca agggtaacaa
     1861 tacaggaaca ttctcggttc tcaagcgcaa tagctttagc ctgcgcgctt caaggtcgcg
     1921 taacggccac ctccagctcc tggtctctta tcgtggcgat cacatccccg tcctcgtcgt
     1981 tgcggaagac cctggcgcct gcccgctgca gcctgtcgag ggtctcgggt gtgggatgcc
     2041 cgtaggggtt gtccaccccg cactggatga cggctacgtc aggcggaaag cggctgagga
     2101 agagcggggt cgaagaggta ttggagccgt ggtgggtgac cttcaatagc gtcagtggac
     2161 ccgtgtacgg cccattcgcc atgtactcct cctcgttcgc ctcagcgtcg cccgcgagca
     2221 ggatacgggc cgtgccgtag gtcaggagga tggccacgga gttgtcgttg atctctgtga
     2281 agagcccacc ctcggcgtcg gttggcggac cgatgacgtc ggcctgtacc ccagcccaat
     2341 ccatccgcat cccggcacgc aagaccttag tacttgctcc ctcgtcgcgc accccgcgca
     2401 aaaacgtgtt gtacgtcagg gtggtgttgg ggtctcctga gacgaagacg gtctcgaccg
     2461 ggaaggcgtc gaacacgtcg aggaagcccc cgatgtggtc tgcgtcgggg ttgctgacca
     2521 ctatgccgtc gagtgcatcg acgcccctgc tgcggaggaa atccaccacg ttcggacctt
     2581 cctccggcct ccccgcgtcg atgaggtagc tctcgtcact cgcctgcacg agcacgccgt
     2641 ctccctggcc aacgtcgatg aaactcacgg ataatgaatc cgacggcggt ggtccaggcc
     2701 cgccgcggga tccgccggtg agcagctcca actccgcgca cgcgggtgac agcaggatca
     2761 gggccaccat cagagccacg aacggtcctc cgtgaaaagc tacgcagggc tcccgcttac
     2821 ctccggtatc gggcgatgga tccatcgatc ggccttccac gtgtgcgtcg cgctgccggc
     2881 tggtcctcgg cgggattctt ttccagcgtc gttcgttacc aggcactgcc caatgatcga
     2941 ccgcgcaccg cgattcctta agttcggcca agagattgtg gggcatgggc cgtgtggcta
     3001 ctcagtaggg tcgtcgcgca gatgctcttc ggaccggact ccgacctcga ggtggcacag
     3061 atgtctccgt ggctcgacct ccgcgtccct ccgaaggccg tcaagctgcc gatggtcgcg
     3121 gcacagacgc accgacggtt cctgaagacg catctccccc tcgatgccct ggtcttctca
     3181 caggaggcga aatacctcta catcggacgc gacgggcgcg acgtcgtgtg gagcatgtac
     3241 aaccaccacg
//
LOCUS       DATAIR010000268         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_17430, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000268 DATAIR010000000
VERSION     DATAIR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17430"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..2013
                     /gene="ilvB"
                     /locus_tag="VHM69_06510"
     CDS             307..2013
                     /gene="ilvB"
                     /locus_tag="VHM69_06510"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX2740080.1"
                     /translation="MENTAFNIEYKPRTGSEAICEALLDEGVEYLFGYPGGAIMPFYD
                     ALPDYPLKHILTRHEQAAAHAADGYARATGRVGVCVATSGPGATNLVTGLATAQMDSV
                     PLVAITGQVGRHAMGMDSFQETNVMGMTLPFTKHSFLVENPDELYPTMRRAFEIARSG
                     RPGPVLIDVPKDVQFAPCNDNGYRRLREAPPPVESPGLERAAMLLRNARRPLILAGHG
                     VTLGGAEMELRLLAERTGIPVVTTLLGISAFPDDHPLYLGWPGMHGQASVNRAIDRSD
                     LLFAVGMRFDDRITGAAHKFAPNAKIIHIDVDPSELGKVIKPTVGIAADAKAAMRSIL
                     GHLDNATEEGEGCARWREELATSQEPERQRQESARDEYGPIQVMDAIKAAAGPDLRLV
                     TDVGQHQMWAAQFFEFTKANSHITSGGLGTMGFALPAAMGVAFAYPEDPVWVVAGDGG
                     FQMNIQELATIREFGLNIKVAVLNNGYLGMVRQWQQFFHNRRYSETPITGPDYEQLTA
                     AYGLAGRTVREGDDVEAAVRWAQEQEGCTILDFHIDPEANVYPMIPSGMSVNEMIEED
                     GA"
     gene            2006..2470
                     /locus_tag="VHM69_06515"
     CDS             2006..2470
                     /locus_tag="VHM69_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740081.1"
                     /translation="MPESVTGVAMNTIEVRFTGGMLALNRLLMTLQNKRMPVAGFTLA
                     RDGDGMRATILLDCPPESALRYTALISALEDVQEAGPAETVEVALIWTSGDWRTAAEQ
                     SGVGAHENGGMVVTSGEPENVEDFLASLGDSVEDIVRLGPLARPEGEARGGN"
     gene            2471..3484
                     /gene="ilvC"
                     /locus_tag="VHM69_06520"
     CDS             2471..3484
                     /gene="ilvC"
                     /locus_tag="VHM69_06520"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2740082.1"
                     /translation="MARVYREGDIGNEITTRRVAILGYGSQGHAHALNLKESGCDVTV
                     GLYEGSQSWPKAEQDGLPVKTIAEAVQGADVIMMLLPDEKQPAVFEAEVGPNLSEGDL
                     MLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYVEGKGMPSLFAVENDASGQA
                     RDVILSYARGIGSGRAGIIETTFAEETETDLFGEQAVLCGGLTALLKAGFDTLVEAGY
                     QPELAYYECVNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTAGPKIIDEAAKERMRG
                     ILADIQSGRWAKDWVLENKAGGSSFLAMRRREAETQVEKVGQELRALAAEGIETPAGG
                     DNR"
     gene            3481..>3738
                     /locus_tag="VHM69_06525"
     CDS             3481..>3738
                     /locus_tag="VHM69_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEX2740083.1"
                     /translation="MSRTAFAASDAEWAYHGGELIRMGDIRLSPATHALNYGTGVFEG
                     IRAYWSEERGTLQVLKMREHYERFEKSCRLLRIDFPSTVDEL"
BASE COUNT          765 a         1162 c         1219 g          592 t
ORIGIN      
        1 accttgacaa ccctctaccg ttgtgtaacc cttcaccaca cttgcgggct aaacgagcga
       61 gggcttttga cggaagtctc gaaaacggcg cagaagaagg tctcgggttt gatgctcaag
      121 atctcacgcc tgctcgctcc ccccatccat ccgcgagtat ccacggtagc cgcaccgtcc
      181 gccctacgac taattagccg ggcgggccgg gacggcgctc tccggtccgc ctagagccgc
      241 aagacgggca cgccccgttc tacggcaagg ccgaggcaag actatttatc tagaacgggg
      301 taaaccgtgg aaaacaccgc attcaacatc gaatacaagc cgcgcacagg cagcgaagcc
      361 atatgcgagg cgctactcga cgaaggggtc gagtacctct tcgggtatcc tgggggcgcg
      421 atcatgcctt tctacgacgc cctccccgat tacccgctga aacacatcct tacccgccac
      481 gaacaggcgg cggcacacgc ggcagacggc tacgcgaggg cgaccggaag ggtcggggtg
      541 tgcgtggcga cgagcgggcc aggggcgacg aacctggtta cagggctggc cacggcgcag
      601 atggactccg ttccgctggt tgccataacg ggtcaggtcg ggcgccatgc gatgggcatg
      661 gactccttcc aggagaccaa cgtcatgggc atgacgctgc ccttcaccaa gcactcgttc
      721 ctggtcgaga accccgacga actctaccca acgatgcgac gggcgttcga gatcgcacgc
      781 tccggcaggc cgggccccgt cctcatcgac gtgcccaaag acgtgcagtt cgcaccctgc
      841 aacgataacg gctacaggag actgcgcgag gccccgcctc ccgtcgagtc gcctgggctc
      901 gaacgtgccg cgatgctcct gcggaatgcc aggcgacccc tcattctggc gggacacgga
      961 gtcactctcg ggggcgcgga gatggagctc aggctgctcg ccgagaggac gggcatccct
     1021 gtcgtgacga cgctgctcgg cataagcgcg ttccccgacg accatccgtt gtacctgggc
     1081 tggcctggga tgcacgggca ggccagcgtc aaccgcgcca tagaccgctc cgacctcctc
     1141 ttcgcggtcg ggatgcgctt cgacgacagg atcacgggcg cagcccacaa gttcgccccg
     1201 aacgccaaga tcatccacat cgatgtggat ccgtcagagc tcgggaaggt cataaagccg
     1261 accgtaggta ttgcggcgga cgcgaaggca gcgatgcgct cgatcctcgg acacctggat
     1321 aacgccacag aagagggcga aggatgcgcc cggtggcgcg aagaactagc gacgagccag
     1381 gaaccggaga gacaacgcca ggagtcggcg cgggacgagt acggtccgat ccaggtgatg
     1441 gacgccataa aggcggcggc aggacccgac ctgcggctcg tcacggacgt cgggcagcac
     1501 cagatgtggg ccgcccagtt cttcgagttc acgaaggcca acagccacat aaccagtggt
     1561 gggctcggaa cgatgggctt cgcgctgcct gcggctatgg gtgtggcgtt tgcctatccc
     1621 gaagatcccg tgtgggtcgt ggccggcgac ggcggtttcc agatgaacat ccaggagctc
     1681 gcgacgatca gggaattcgg tttgaacatc aaggtcgccg ttttgaacaa tgggtacctg
     1741 gggatggtcc ggcagtggca gcagttcttc cacaaccgcc gctactctga gacgcccata
     1801 acgggacctg attacgagca gctcaccgca gcctacggcc tcgctggacg caccgtgcgc
     1861 gagggcgacg acgtcgaggc tgccgtgcgg tgggcgcagg agcaggaagg ctgcaccatc
     1921 ctggacttcc acatcgaccc cgaggcgaac gtctatccca tgatcccatc gggcatgtcc
     1981 gtcaacgaga tgatcgagga agacggtgcc tgaatcagtc accggcgtgg ctatgaatac
     2041 catcgaagtc cgtttcacag gcgggatgct cgccctgaat cgcctcctga tgacgctcca
     2101 gaacaaaagg atgcccgtcg ccggcttcac gctagcccgc gacggagacg gaatgcgggc
     2161 caccatcctg ctcgactgcc cgccggagtc cgcgctgcgc tacacggccc tcatctcggc
     2221 cctcgaagac gtgcaagagg ccggaccggc agagacggta gaggtagccc tgatctggac
     2281 gtccggagac tggcgcacgg ccgcggagca gtcaggcgtc ggagcccacg agaacggcgg
     2341 gatggtcgtg acttcaggcg agcccgagaa cgtcgaggat ttcctcgcct ctctgggcga
     2401 tagcgtagaa gacatcgttc ggctgggtcc tttggcccgt ccggaaggcg aagcccgagg
     2461 agggaattag atggcacggg tataccgcga aggagatata gggaatgaga tcacgactcg
     2521 gagagtcgcg atccttggtt acggcagcca gggccacgcc cacgccctga acctgaagga
     2581 gtcgggctgc gacgtgaccg tcgggctata cgagggctcg cagagctggc cgaaagccga
     2641 gcaggacggc ctgccggtga agactatcgc cgaggctgta caaggggcgg acgtaattat
     2701 gatgctcctc ccagacgaga agcagccggc cgtcttcgag gccgaggtcg ggccgaacct
     2761 ctccgagggc gacctcatgc tcttcgccca cggcttcaac atccatttca accagatcgt
     2821 cgttcccccc gaggtagacc tcggcctcgt cgccccgaag ggaccgggcc acgtactcag
     2881 acgcctctac gtcgaaggca aggggatgcc ctcgctcttc gccgtggaga acgacgcctc
     2941 ggggcaggcg agggacgtaa tcctctccta cgcccgcggc atcgggagcg gtcgcgcagg
     3001 gatcatcgag actaccttcg cagaggagac cgagaccgac ctcttcggcg agcaggccgt
     3061 cctctgcggc ggattgacgg cgctcctcaa ggccgggttc gacacgctgg tggaggcagg
     3121 ttaccagcct gagctcgcgt actacgagtg cgtaaacgag ttgaagttga tcgtcgacct
     3181 catctacgag ggcgggctgg caggtatgcg gtactcgatc tcgaacacgg cagagtacgg
     3241 cgactacact gccgggccga agatcatcga cgaagccgcc aaagagagga tgcgcggcat
     3301 cctcgctgac atccagagcg gcaggtgggc caaagactgg gtactcgaga acaaggccgg
     3361 gggttcgagc ttcctcgcga tgcgccgcag ggaggccgag acgcaggtcg agaaggttgg
     3421 acaggagttg cgggcgctcg cggctgaggg catcgagaca cccgctggag gtgataacag
     3481 atgagtcgta cagcattcgc tgcgtcagac gcagagtggg cctaccacgg cggcgagtta
     3541 atcaggatgg gagatattcg cctctccccc gcgacccacg ccctgaatta cggcaccggc
     3601 gtcttcgagg gcatccgtgc gtactggagc gaggaacgcg ggacgctaca ggtcctgaag
     3661 atgcgcgagc actacgagcg cttcgagaag tcctgccggc tgctacgcat agatttcccg
     3721 agcaccgtcg atgagctg
//
LOCUS       DATAIR010000269         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_54054, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000269 DATAIR010000000
VERSION     DATAIR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54054"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VHM69_06530"
     CDS             complement(<1..632)
                     /locus_tag="VHM69_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2740084.1"
                     /translation="MFERFTERARKVVVLAQDEARHFNHNYIGTEHLLLGLLREDEGV
                     AAQALSSLNVSLDEVREQVESIVGYGEEGTGAQAPFTPRSKKVLELALREALQLGHNY
                     IGTEHILLGLVRESEGVAARVLSNLDVDPDKVRREVVRRLGGGRSRSRGGGEAGGRGA
                     EAKRPKTRQLDQYGRNLTAYAEEDKLDPVIGRSQEIARIMQILVRRTKNNP"
     gene            complement(954..2423)
                     /gene="lysS"
                     /locus_tag="VHM69_06535"
     CDS             complement(954..2423)
                     /gene="lysS"
                     /locus_tag="VHM69_06535"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX2740085.1"
                     /translation="MNETGIRIPGWAERVAENLGDGPHVVVTGISTSGNIHVGNLREV
                     LVGEAVANALRARGAEVRFIFHADTIDPLRKIAPGIPEHFEEYIGHSLSRVPDPEGCH
                     VSYAEHFLAPFEEALAQMEMDIEVVRSHELYESGVYTWAIREALEHTDELRDILQDVS
                     GRQMPEHWSPFLPRTASGKLTGARVLEHVAEEKKVVYVDEDGWEDAADYSRGEGKLGW
                     RVELAARWKALGVTFEPFGKDHTSRGGSTETADRMSGEVFDYPVPGRYEYEWIGIKGR
                     GDMSSSKGIVLLPKDLLRIMPPEALRRMVLGRDPARRSDLELGEGFPRFMDEYRAEVG
                     EPYVPFTHLVTVAQTVGEVTDAAAEMLRRGGYEEAAEDRDKLARDLYYARNWAREWAP
                     ESMRLGLLDSDESREEAAGLDDEQREYLREVSEKLTPEMDGEAVQDVLYSTAIERGIK
                     PKRAFAAVYKVLLGKSSGPKAGPFIAGLTLDQVRQRFSV"
BASE COUNT          401 a          829 c          778 g          517 t
ORIGIN      
        1 gggttgttct tggtgcgcct gacgaggatc tgcatgatcc tcgctatctc ctggctcctg
       61 ccgatgacgg ggtcgagctt gtcttcctcg gcatacgccg tgaggttcct gccgtactgg
      121 tcgagctgcc tggtcttggg acgcttggcc tcggccccgc gtccgccggc ctcgccgccg
      181 ccccgactgc ggctgcggcc gccgccgagc ctgcggacga cctcgcggcg gactttgtct
      241 ggatctacgt cgaggttgga gagaactcgg gccgcgacgc cctcgctctc ccgtaccaga
      301 ccgagcagga tgtgctcggt gccgatgtag ttgtggccca gctgcagagc ttctctcaga
      361 gcgagttcga gcaccttctt cgagcgcggc gtgaaaggcg cctgcgcgcc ggtgccttcc
      421 tcgccgtaac cgacgatgct ctcgacctgt tcgcgcacct cgtcgaggga gacgttcagg
      481 gaggagagcg cctgggcggc gacaccctcg tcctcgcgca ggaggccgag gaggaggtgc
      541 tcagtgccga tgtagttgtg gttgaagtga cgggcctcat cctgcgcgag caccacgacc
      601 ttgcgggccc gctccgtgaa tcgttcgaac attagcggga tcctcctcta tttcgagggt
      661 ccttcacggc gtcgggggat ataacggtct gtatctgtat acgagtgtcg aagataggtg
      721 gattatacat ctgcaaaaat ctgcctttcg aaatgacgtg cgcctctcta ataagtgcct
      781 ctctcgctcg tgacggctga cccaaagtat acatccgttg ggccagtatt tcggcaccag
      841 ccagaacctt tacgctctga gcgctatacc tcgtgtggcg gtggggcgaa tggtcctttt
      901 gtagcgcttt ctatgagaat accgccgccg agccatctat tcggtgtctc gtctcacacc
      961 gagaacctct ggcggacctg atccagggtc aaccccgcga tgaaaggccc ggccttgggt
     1021 ccgctgctct tgccaagcag tactttgtag acggctgcga acgccctctt gggcttgatc
     1081 ccgcgctcga tggcggtcga atacagtacg tcctggacgg cttctccatc catctcaggc
     1141 gtgagcttct cagatacctc gcgcaggtat tcgcgctgct cgtcatcaag cccggcagcc
     1201 tcctccctcg actcgtcaga gtcgagcaac ccgagccgca tcgactcggg agcccactcc
     1261 ctggcccagt tgcgggcgta gtagaggtcc ctcgcaagtt tgtccctgtc ctcggcggcc
     1321 tcctcgtacc cgccgcgacg cagcatctca gcggcggcgt cggtgacttc gcccacggtc
     1381 tgagcgacgg tgaccaggtg ggtaaaagga acatagggtt cgcccacctc cgcgcggtac
     1441 tcgtccataa agcgcgggaa gccttcgccg agttcgaggt cggacctgcg ggccgggtcg
     1501 cgaccgagca ccatccgccg cagagcctcg ggaggcatga tcctcaggag gtccttcgga
     1561 agcagcacta tgcccttcga gctgctcatg tcgcccctgc ccttgatccc gatccactcg
     1621 tactcgtagc ggccaggcac agggtagtcg aagacctccc ctgacatgcg gtcagccgtc
     1681 tccgtggacc caccccggct tgtgtggtct ttgccgaagg gctcgaaggt cacgccgagc
     1741 gccttccacc gcgccgcgag ttcgaccctc cacccgagct tgccctcgcc ccttgagtaa
     1801 tccgcagcgt cctcccagcc gtcctcgtcg acgtagacga ccttcttctc ctcagccacg
     1861 tgctccagga cgcgggcccc cgtcagcttc ccggacgcag tgcgcggcag gaaaggagac
     1921 cagtgctcag gcatctgcct gccgctgacg tcctgcaaaa tgtcgcgtag ttcgtctgtg
     1981 tgttccagag cctccctgat ggcccaggtg tagacgccgc tctcgtagag ttcgtgcgac
     2041 cggacgacct cgatatccat ctccatctgt gcgagcgcct cctcgaaagg cgcgaggaag
     2101 tgctcggcgt aggacacgtg gcaaccctcg gggtcgggga cgcgggagag gctgtggccg
     2161 atgtactctt cgaagtgctc ggggatgcct ggtgcgatct tgcgcagggg atctatggtg
     2221 tccgcgtgga agatgaaacg gacctcggcg ccccgcgccc tcagggcgtt cgccaccgct
     2281 tcgccaacga gaacctcccg caggttgccg acatggatgt tgccggaggt gctgatgccc
     2341 gtgaccacaa cgtggggacc atcgcccagg ttctcggcga ccctctcggc ccagccgggt
     2401 atgcggatgc cggtctcgtt catgcggtgc tccatgtaaa gtcggttgtg gggatcgtgg
     2461 agaggtctgt cagcgaccta gctagccgcc tcgacgttga cgatctcgta ctccgtcgcg
     2521 ccgcg
//
LOCUS       DATAIR010000270        13336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_17532, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000270 DATAIR010000000
VERSION     DATAIR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13336
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17532"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHM69_06540"
     CDS             complement(<1..191)
                     /locus_tag="VHM69_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX2740086.1"
                     /translation="MRLDALLVERGLAESRSRAEAMILSGSVRVRDRLVTKAGSRVAP
                     GEPLSVERSERFVSRAGEKL"
     gene            complement(240..1142)
                     /locus_tag="VHM69_06545"
     CDS             complement(240..1142)
                     /locus_tag="VHM69_06545"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX2740087.1"
                     /translation="MASGASGSQIRWEGHRRTFEKYLHGLRFTEEPRLGALEEAMRYS
                     MLGGGKRVRPALCMETAWIFGADPSIVLPSAAAIELIHTYSLIHDDLPAMDDDDYRRG
                     RPTAHKKFGEAMGILAGDAFFGEALTLITVHQKGSCEQVLEVVRDLADSTGVNGMVGG
                     QVLDMDHTGQGEVTEPETLYMIHRYKTGALIKSSARIGAILAGATHEEQAAVSEYASE
                     LGLCFQIVDDVLNATSTSEELGKSAGSDAEQGKATFVGVFGLEGATREADGAAKRALG
                     ALSTLDGDTSGLQELAYFVRNRES"
     gene            complement(1126..1545)
                     /gene="nusB"
                     /locus_tag="VHM69_06550"
     CDS             complement(1126..1545)
                     /gene="nusB"
                     /locus_tag="VHM69_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2740088.1"
                     /translation="MSRRTARKQAFLNLYQSDVNDSPLGTTMQRWREYRGDLEEYAVN
                     LALGVEQEQVHLDEVLSEVSVGWPVHRMSAVDRSILRLALYEMLYVDDVPPDVAVNEA
                     VELARGFSGDESPAFVGGVLRGAEVLARGKVGHGERG"
     gene            complement(1551..2108)
                     /gene="efp"
                     /locus_tag="VHM69_06555"
     CDS             complement(1551..2108)
                     /gene="efp"
                     /locus_tag="VHM69_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX2740089.1"
                     /translation="MISTNQFRNGTAIRVDGKRFTILFFQHVKPGKGGAFVRTRLRNI
                     DSGAVVEKTFRAGEKLENVRTESRQMTYLYRDGDLLYFMDSDNYEQVPVPVDVVGEAV
                     GYIVEGSTVSVLSADGEVVSVEPPAHVDLEVSETDPGLKGDTAQGGNKPATLETGVVV
                     QVPLFVNVGDSVRVDTRSNEYLTRV"
     gene            complement(2239..2673)
                     /locus_tag="VHM69_06560"
     CDS             complement(2239..2673)
                     /locus_tag="VHM69_06560"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX2740090.1"
                     /translation="MSKVFVLNGVNLGTLGTRRPEVYGTMTLDDIERLLMDEFQGLDL
                     EFRQTDFEGEMVGFIREADGSGGLVINAGAWTHYSYALHDALEAVGVPKVEVHLSNVH
                     TREAWRRVSVISPAVDAVIAGMGVHGYVAGVRYVLQREGADA"
     gene            complement(2666..3751)
                     /gene="aroB"
                     /locus_tag="VHM69_06565"
     CDS             complement(2666..3751)
                     /gene="aroB"
                     /locus_tag="VHM69_06565"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX2740091.1"
                     /translation="MPVEKIEVPVAYPYPVIVGEGLDLGVVLSDSLRPGLCAIFTDLT
                     VGPLHAAKVRATLENSGWRVEGVIEVPSGESSKSMEAYAEALRRLARLGLTRDSTLFA
                     LGGGVVGDLGGFVAGTYMRGIDLVMLPTSLLAMVDSSVGGKVGVDLPEGKNLAGAFVR
                     PRLVAADLDWLGTLPAREISNGLAETIKMGLLSGGEYFEDLDRIEAARDGDLAAMHAL
                     VLHSIRFKAQVVAEDELESGLRAILNYGHTIGHALEAAAGYNLPHGTAVAAGMVAAAQ
                     LSRERFGNDLTGTHEDLLRSAGLPRRVPDVGVDRALQAMARDKKRQGADGSSQHRFVL
                     LQDIGKPVRNVPVGDDEARQAIGAVLE"
     gene            complement(3733..4236)
                     /locus_tag="VHM69_06570"
     CDS             complement(3733..4236)
                     /locus_tag="VHM69_06570"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX2740092.1"
                     /translation="MDGTLAIVGYMGSGKSTLGRVLARDLNREFVDLDRAIVKKAGKG
                     IPEIFAEQGEDAFRDIEHETLIYAIRGRTPTVVACGGGIVVRNDNRALLRDVTTVFLR
                     EDTDVLYNRTRGPGRPLRASSREEFERRYTERLPLYREVADLEIEVDGRPTAQVAEEV
                     LACLSKR"
     gene            complement(4240..5403)
                     /gene="aroC"
                     /locus_tag="VHM69_06575"
     CDS             complement(4240..5403)
                     /gene="aroC"
                     /locus_tag="VHM69_06575"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2740093.1"
                     /translation="MRFGFSTAGESHGPAEVALVHGVPAGLGLLAADVNRDLARRQKG
                     YGRGGRQKIEKDEVEFLGGVRHGYTLGSPVAMLIRNMDYANWEHRMTPEPVEDPPDKI
                     TLPRPGHADLAGMQKYDFDDLRNVLERSSARETTARVAAGGVARKLLAEFGVEISSAV
                     YRIGAVAYPKERAALDSSRSDESEVRCPDPDISEKMKAEIDAARYARDALGGEFVVVA
                     RGCPPGLGSYVDWRDKLDARLAAAMMSINAIKGVEFGMGFDLAARRSSEVQDEIVSEE
                     GRLARNSNRLGGLEGGMSNGEPVIVAAAMKPISTIAKALRTVDLQTGEPTRAFKERAD
                     SCAVPAAAVIGESMAAIVLAEAFLEKFGMDNMTDIRAAYDAYTRRVAKTGKRD"
     gene            complement(5579..6244)
                     /locus_tag="VHM69_06580"
     CDS             complement(5579..6244)
                     /locus_tag="VHM69_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740094.1"
                     /translation="MSGVLQRMSSIGSGFMDTFVRNNRVLPPVLALLALFVFAWILAG
                     AFFDEPGQEPVAHRDDLAQSNADPAAPEIENRDVDSYAAYRNKDPFRQLIAPAQAPSE
                     QTTPEGTTDQTTPEETTGTPPDDSSGNDAGGARPADADNDGVPNRREQALGLNPRKPD
                     SDGDGTPDGEDDANGDGRPDGTAGNGAGGKNGPADGSGGRDGRRPGRSGDLLDSGGIL
                     YPR"
     gene            complement(6246..6878)
                     /gene="pilO"
                     /locus_tag="VHM69_06585"
     CDS             complement(6246..6878)
                     /gene="pilO"
                     /locus_tag="VHM69_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX2740095.1"
                     /translation="MSRNDRNMLILGSLGIVLVVVAFYFLLLSPLLARLDEQAQARED
                     KEAQLAQLQQEVNELEEVRRNSPEIERQLLELNKRIPTQPEVPTLVVQIEEIADAAGV
                     TQVSIEPGPPGPPQGGGEFSVQPITMSFEGTYDQLQEFLLRTRNLARLVTVNSVNYEA
                     AEAEATTAEPTIEQTLQVVIEADVYFQPTGVPSGEAPVAPETTTQSTTGG"
     gene            complement(6875..7627)
                     /locus_tag="VHM69_06590"
     CDS             complement(6875..7627)
                     /locus_tag="VHM69_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740096.1"
                     /translation="MRRINLLPPEERGRPGERFRGGVLGILLIAGALALIVMVGLYLI
                     MLMRLGDLEDRVAELDDEIAQQNAHLAELSPYRDLKTRLEEKKPVADGIYRTRFAWDQ
                     FLQGLAFVIPESTALQTLTAEAAPIDIDAPVEETLDPPGAVTFTGIALPRYENVADFV
                     VRMNNLEFLANSELNQAELDRQTFQQPAINFEVASELVTMVGENGTEVRLDGGSRDEV
                     AGNGTQEVASLEFLNADASRDPHAGRAGDEQR"
     gene            complement(7624..8742)
                     /gene="pilM"
                     /locus_tag="VHM69_06595"
     CDS             complement(7624..8742)
                     /gene="pilM"
                     /locus_tag="VHM69_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX2740097.1"
                     /translation="MERLRRFRRVGSRKNIGIDIDAGSIKAVQVSGSGGGYVLQHVGY
                     HRLPPGTVIEGEVADHDLLAAEIKEFWDSHSFKGKSVTIGVSNQKVVVRLLDFPHMEP
                     DDLQGAISFEAQDHIPMPLDEAVLDYVVLGPREERSDLDRVLVVAAQREMLSRYTSAV
                     RTGGLRPSGVDVKALSLTRSTLPDPFFGEEGAVLLLDVGAEITNLVVADLGNPVLTRF
                     VPVGLGDFVAAVAELANLPQDEAEKQALNSRVMMGDEEDVADETQSDGFDPALAFDVR
                     RGLESAGQALADEVQRSIEHHRSQEVAREVSRVLISGEAALIPGLDRYLGELLNVPTG
                     RGNPVEKLSANRSNISDEQLKAMEPVLAVALGLAMEDE"
     gene            complement(8748..9494)
                     /locus_tag="VHM69_06600"
     CDS             complement(8748..9494)
                     /locus_tag="VHM69_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX2740098.1"
                     /translation="MIAPASAVLGLVVGSFLNVVIHRVPLKRSVVWPSSRCPQCGEPI
                     SPKDNIPLVSYLLLRGRCRSCGERIPMRYPVIEALTALLFGAAGYQFGASIQLISALV
                     LISALISLAAIDLEHGLLPNAIVLPCAALGLVLSVIRDPDGWWIYVISAFAIGGGLLV
                     LALAYPAGMGMGDVKMGGMLGAFLGPYGALAVFLGALCGTFVAVILLALGRIRRRSAL
                     PFGVFMALGGLVTLFQGPEIFALYLRLAGI"
     gene            complement(9491..10063)
                     /locus_tag="VHM69_06605"
     CDS             complement(9491..10063)
                     /locus_tag="VHM69_06605"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740099.1"
                     /translation="MGAGGYGRVALDVLLASGLGDRVLGFYDDAHAAISEKVRGYPVL
                     GDIEMLKSMLSVEPVHIVVAITDNGARLRVANSLRALGGQFFTVVHPLAYLSDVATVE
                     GGCVVSSGVVVHPDAAVGSHCFLGPACVVDRDAEVGAGSWISAGAVVGSGARVGARVV
                     LGPNSSVGRKASVGADVEVSALRHVAREGA"
     gene            complement(10282..11199)
                     /gene="fmt"
                     /locus_tag="VHM69_06610"
     CDS             complement(10282..11199)
                     /gene="fmt"
                     /locus_tag="VHM69_06610"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2740100.1"
                     /translation="MKVAFAGTPDFAATVLRGLISSPHEVGLVISQPDPRKGRGRKTT
                     QAPVAEVAASHGLTLRQPARISEISDELSQYDALVVAAYGQILRPDTLYAARNGALNV
                     HASLLPKYRGAAPVERAIMNGETETGVTIMQMDEGLDTGPIAIQRHVPISPDMTGGEL
                     ADLLAHIGADAIVESLTLVERDALTLIEQDSLYATYASKVLDEDRVIKWEREVGEVHD
                     LIRALAPHIGARTFHPMVDGPVKIWRSRILDKERSSTEIGRIKAENGSILVSCGLGTL
                     EVLELQLPGGRRIAARDYLLGNSLEGAFR"
     gene            complement(11209..11706)
                     /gene="def"
                     /locus_tag="VHM69_06615"
     CDS             complement(11209..11706)
                     /gene="def"
                     /locus_tag="VHM69_06615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2740101.1"
                     /translation="MALEIRTFGDPVLKTRAAPVESFDESLLRLTEDMLITMREYEGV
                     GLAANQVGRLKRVLVAAIEDDEYVIVNPVLTDAAETTETGPEGCLSIPGIQVEVERPI
                     AVTVSGQDTSGKPLRIEATDLLARVLQHEVDHLDGVLILDRTDRESRKAAMREWRERL
                     LAQQG"
     gene            complement(11712..>13336)
                     /locus_tag="VHM69_06620"
     CDS             complement(11712..>13336)
                     /locus_tag="VHM69_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740102.1"
                     /translation="LLSETGASRSALRELVRRGAVRLVRRPGPAQLLATMGDGAVPEH
                     LSSFTRNAGRAVDSGGTFLWRTARSEELDAVTTIAKATLERGDQALILAPEIAVVERM
                     VDTLRRELPAGHTVAAYHGGLGRGRAAVYEAARAGEVDVVVGTRTAALLALDRPGSIC
                     VVDEPNEAHRAGPGHEGLPLHVRDVALERSRLERAAVFFLSPFPSLRLYAVEVRKRER
                     IRELPASRSVRWPAVRIVDMRGSGATFSSTLIDACRRCVDEGGKVGLILKRLGYATAV
                     SCSHCGAVKRCPKCDLPLVMHGRGGPLICSRCGYREKAGPCTQCGSSRVRPTGLGVER
                     VRDELSELLGERVGLITADEQDHADASVVVGTAPRILDSEWDAVILPDIDAFLAGNNI
                     GAVEKSFRLLYGAAEAAKELLLIQTHLPEHYALRAGVRGDYEKFAAAELSRLRTLGYP
                     PFAHVAYLDLEGSEEVVSVTVKSRVRPALEPGVEMSAPVPLRRTGGTTVWRVLLRSRG
                     RPAVARAAALAARMAARTHGLKVRVEVDPEEV"
BASE COUNT         2342 a         4460 c         4010 g         2524 t
ORIGIN      
        1 agcttctcgc cggcgcgcga cacgaagcgt tcgctcctct ccacggagag gggttcgcca
       61 ggggccacgc gcgagcccgc cttggtcacc aggcggtcgc gcacgcgcac gctgccggag
      121 aggatcatag cctccgcacg gctcctgctc tcggcgagac cccgttcgac gagcagggcg
      181 tcgagccgca tgcggccccc atgctggctc cctctcgggc tacttttcat gccggacggc
      241 tagctctccc tgttgcgcac gaagtacgca agctcctgca ggcccgaggt gtccccgtcc
      301 agggtcgaga gcgcaccaag ggccctcttc gcggccccgt cggcctccct tgtggcgcct
      361 tcgaggccga agacgccgac gaaggtcgct ttgccctgct ccgcgtccga accggcgctc
      421 ttgccaagct cctcgctggt cgatgtggca ttaagtacgt catcgactat ctggaagcaa
      481 aggcccagtt ccgaggcgta ctccgagacg gcggcctgct cttcgtgtgt ggcccccgcc
      541 agaatcgccc cgatacgggc tgaggatttg atcagggctc cagtcttgta cctgtggatc
      601 atgtacaggg tctcgggctc tgtcacctca ccctgacctg tatggtccat gtcgaggacc
      661 tgtcccccga ccatcccgtt tacgcctgtc gagtcggcga gatcgcgcac gacctcgagt
      721 acctgctcgc aactcccttt ctggtggacc gtgatgaggg tgagtgcctc gccgaagaag
      781 gcgtcgcccg cgagtatgcc catcgcttcc ccgaatttct tgtgagcggt cggcctgccc
      841 ctcctgtagt cgtcgtcgtc catggcgggt aggtcgtcgt ggatgaggga gtaagtatgg
      901 atgagttcga tagccgccgc ggaaggcagc acgatggaag gatcagcacc gaagatccag
      961 gccgtctcca tgcacagcgc gggccttaca cgcttgccgc caccgagcat cgaatacctc
     1021 atggcctcct cgagcgcccc gaggcgcggc tcctcggtga accggagccc gtgaagatat
     1081 ttctcgaagg tcctcctgtg tccctcccac cggatctgcg acccgctagc cccgctcgcc
     1141 atgccccacc ttgccgcgcg caagtacttc ggccccacgc aagaccccgc cgacgaacgc
     1201 aggcgactcg tcgcctgaaa atcctctcgc gagctccacc gcctcgttta ctgcgacgtc
     1261 agggggtacg tcgtccacgt agagcatctc gtagagggcg agtcgcagta tggaacggtc
     1321 cacagcgctc atgcggtgca cgggccaccc cacagagacc tcggagagca cttcgtcgag
     1381 atgaacctgc tcctgctcga cgccgagggc gagatttacg gcatactcct caaggtcgcc
     1441 cctgtactcg cgccagcgct gcatggtcgt gccgagcggg ctgtcgttga cgtcgctctg
     1501 gtacaggttc aggaacgcct gcttgcgggc agtccggcgg ctcaagtgac tcagaccctc
     1561 gtcaggtact cgttggagcg cgtgtcgacc cgtacgctgt cgccgacgtt gacgaagagc
     1621 ggcacctgta cgacgacccc ggtctccagg gtggctggct tgtttccccc ctgggccgta
     1681 tcgcccttga ggccaggatc tgtctctgag acctcgaggt cgacgtgcgc cggcggctcc
     1741 acactaacga cctccccgtc agccgagagc acacttaccg tagagccctc gacgatgtac
     1801 cccactgcct cacctacgac gtccaccggc acgggcacct gctcgtagtt gtccgagtcc
     1861 atgaagtaca acaggtcacc gtcgcggtag aggtacgtca tctgccgcga ctcggtccgc
     1921 acgttctcca gcttctctcc agccctgaag gtcttctcga ccacggcccc actatctatg
     1981 ttcctcaggc gcgtcctgac gaacgccccg cccttgcccg gcttgacgtg ctggaaaaac
     2041 aggatcgtga accgcttgcc gtctacccga atggcagtgc cgttcctgaa ctggttcgtg
     2101 gatatcatgc tcgaaatctc cccttcaaag cgccaggtcc caaccatacc gtgctacgta
     2161 aacccggtgc agacgccgcg acattatcgc acaattttcc gggctttaag cctcatctcc
     2221 accggtgatc cccctgcttc acgcgtcggc accttccctc tggaggacgt acctgacgcc
     2281 ggcgacatat ccgtggaccc ccatgccggc tatcacggcg tccacagccg gtgagatgac
     2341 cgatacccga cgccacgcct cccgcgtgtg cacgtttgat agatgaacct cgactttcgg
     2401 tacacccacc gcttccagag catcatgcaa agcgtaagag tagtgggtcc aggcgcccgc
     2461 gtttatgacg agcccgccag aaccgtcggc ctcccgaatg aatccgacca tctcaccctc
     2521 gaaatccgtc tgcctgaact cgagatccag accctgaaac tcatccatga gtagtctctc
     2581 tatgtcgtcg agggtcatgg ttccgtatac ttcgggacgg cgggtgccca gagttccgag
     2641 gttcacgccg ttgaggacga ataccttact caaggactgc tccgatcgcc tggcgggctt
     2701 catcatcgcc cacggggacg ttcctgacgg gcttgccgat gtcttggagg agcacgaacc
     2761 tgtgctgtga gctcccgtcg gcaccctggc gtttcttgtc acgggccatc gcctgcagcg
     2821 cccgatcgac gccgacatca ggcacccggc gaggcagccc tgctgaacgc aacaggtctt
     2881 cgtgcgtgcc ggtcaggtcg ttaccgaacc gttcgcgcga gagctgggcg gcggcgacca
     2941 tgcccgcggc gaccgcggta ccgtgcggca ggttgtagcc cgccgcggct tcgagggcat
     3001 ggccgatagt gtgaccgtag ttcaggatcg cacgcagccc gctttccagc tcgtcctcgg
     3061 cgacaacctg tgccttgaag cggatggagt ggaggacgag ggcgtgcatt gcggcgaggt
     3121 ccccgtccct cgctgcctcg atcctgtcga ggtcttcgaa gtattctccc cctgaaagaa
     3181 gtcccatctt gatcgtctct gcgaggccgt tggagatctc acgcgctggc agcgtgccta
     3241 gccagtcgag gtccgccgct accagacgtg gcctgacgaa agctccggcg aggttcttgc
     3301 cttccggcag gtctacgccg accttcccac cgaccgagct gtcgaccatc gcgaggagcg
     3361 acgtcggaag catgacgagg tcaattcccc gcatgtaggt accggcgacg aagccgccaa
     3421 ggtccccgac caccccgccg ccgagggcga agagggtact gtcgcgggta agccccagcc
     3481 tggcgaggcg cctgagggct tcggcgtaag cctccatgct cttcgaggat tcgccggatg
     3541 ggacctcgat caccccttct actcgccagc cgctgttttc gagggttgcc cggaccttcg
     3601 ccgcgtgcag cggtccgacc gtcaagtccg tgaagatcgc gcacaagcca ggccgcagcg
     3661 aatcagagag gaccacacca aggtcgaggc cctcgccgac gatgacggga taaggatagg
     3721 cgacggggac ctctatcttt tcgacaggca cgcgagcacc tcctctgcta cctgcgccgt
     3781 cgggcgtccg tcgacctcta tttcgaggtc cgcgacctcc ctgtacaagg ggagacgttc
     3841 cgtatagcgg cgctcgaact cctcacggct ggaggcccgc agtgggcggc caggaccgcg
     3901 ggtcctgtta tagagaacgt cggtatcctc cctcaagaac accgtggtca cgtcccgcag
     3961 cagcgcgcgg ttatcattgc gaaccacgat gccgccgccg caggcgacga cggtgggcgt
     4021 ccggccccgg atcgcgtaga tcagagtctc atgttcgatg tccctgaagg catcctcccc
     4081 ttgttcggcg aagatctccg gtatcccctt ccctgccttc ttcacgatcg cgcggtcgag
     4141 atcgacgaac tcccggttca ggtctctggc caggacgcgg ccaagcgtgc tcttgccgct
     4201 tcccatgtac ccgactatag ccagggtccc atccaatcct cagtccctct tccctgtctt
     4261 cgcgacgcgc cgtgtgtagg cgtcgtaggc ggcgcggatg tcagtcatgt tgtccattcc
     4321 gaacttctcg aggaatgctt cggccagcac gatcgcggcc atggactccc ctatcaccgc
     4381 ggccgccggt acggcgcagg agtctgcccg ttccttgaag gcgcgggtcg gctctccggt
     4441 ttggaggtcc accgtacgaa gggccttcgc aatcgtcgag atcggcttca tcgccgcggc
     4501 gacaatgacg ggctctccgt tcgacatccc gccctccagc ccgccgaggc ggttactgtt
     4561 gcgtgccaga cgcccttcct cagagacgat ctcgtcctgt acctcgctcg accttcgggc
     4621 ggccaggtcg aagcccatcc cgaactcgac gcccttgatc gcgttgatag acatcatggc
     4681 ggcggcgagc ctggcgtcca gcttgtctcg ccagtctacg taggagccga ggccgggagg
     4741 gcaaccgcgc gccacgacca cgaactctcc gcctagcgcg tcgcgcgcgt agcgggcggc
     4801 gtcgatctcg gccttcatct tctcagagat gtcggggtcg gggcagcgta cctcggactc
     4861 gtccgagcgg gacgagtcga gcgcggccct ttctttcggg taggcgacgg caccgatcct
     4921 gtagacggcg ctgctaatct ccacgccgaa ctcagcaagg agcttcctcg ccacgccgcc
     4981 cgcggccacc ctggccgtcg tctcccgggc gctggagcgt tccaggacgt tgcgcaggtc
     5041 gtcgaaatca tatttctgca tccctgcgag gtcggcgtgt cccggtcggg gaagtgtgat
     5101 cttatccggt ggatcctcga cgggctctgg ggtcatgcgg tgttcccagt tcgcgtagtc
     5161 catgttgcgg atgagcatgg cgaccggcga gcctagagtg tatccgtggc gtacgccgcc
     5221 gaggaactcg acctcgtcct tctcgatctt ctggcgccca ccacgaccgt aacccttctg
     5281 cctgcgggcg agatcccggt tcacgtccgc tgcgaggaga ccgagacccg ccggaactcc
     5341 atgcaccagg gctacctcgg cgggaccgtg cgactccccc gccgtcgaaa aaccgaatct
     5401 cacaaacgcc tccgagcctg tacgatggac aatctactgc aacgctaata taaaccacga
     5461 gcaagcgtag cacccgctcc agacggacgt aatggttgcg acttacgagg gtaacggtat
     5521 tccggactgc agggttcccg aatattcggg cggccggaca ccgctccggc acgttgacct
     5581 atcgcgggta caatattccg ccgctgtcga gcagatcgcc gcttcggcct ggccggcgac
     5641 cgtcgcgtcc acctgagccg tcggcgggac cgttctttcc gccggctccg ttacctgccg
     5701 tgccgtcggg ccgtccgtcg ccgtttgcgt cgtcttcgcc gtcgggagtt ccatctccgt
     5761 cagagtcggg tttgcgcggg tttagtccca gggcctgttc cctgcggttg ggcacgccgt
     5821 cgttgtccgc gtctgcgggc ctggcgccac cagcgtcgtt gcctgaggaa tcgtccggcg
     5881 gcgtccctgt cgtttcctcc ggcgttgtct gatccgtcgt cccctccggg gtggtctgct
     5941 cggaaggtgc ctgggcaggg gcgatgagct gcctgaaggg gtccttgttg cggtaagcag
     6001 cataagaatc cacatcgcgg ttctcgatct ccggggcggc gggatctgcg ttcgactggg
     6061 ccaggtcatc ccgatgggcg accggctcct gaccaggctc gtcgaaaaag gcgcctgcca
     6121 ggatccaggc gaacacgaac agggcgagca gggccagaac cggcggcaat accctgttgt
     6181 tgcgcacgaa cgtgtccatg aaccccgaac ctatagacga catccgctgt aggaccccgc
     6241 tcaccctaac caccagttgt cgactgtgtc gttgtttcag gcgcgaccgg tgcctcaccc
     6301 gacggcacgc cggtaggttg gaagtacacg tccgcctcta tcacaacctg tagcgtctgc
     6361 tctatcgtcg gttccgccgt ggtagcctcc gcctccgccg cctcgtagtt caccgagttg
     6421 accgtaacca ggcgcgccag gttgcgcgtc cggagcagga actcctgcaa ttgatcgtag
     6481 gtgccctcga acgacattgt aatcggctga acggagaact cgccaccccc ttgcggcggt
     6541 ccagggggtc caggctcgat agagacctga gtcacccctg ccgcgtcggc gatctcctcg
     6601 atctggacga cgagcgtggg gacctccggc tgggtcggga tccgcttgtt caactcgagg
     6661 agctggcgtt cgatctcggg cgaattgcgc cttacttctt cgagctcgtt tacctcttgc
     6721 tgcaactgcg caagttgcgc ctctttgtct tcacgcgcct gggcctgctc atcgagccgc
     6781 gccagcagcg gactgagcag gaggaagtag aaggcgacga cgacgagcac gatgccaagc
     6841 gagccgagga tgagcatgtt gcggtcgttg cgactcaccg ttgttcgtcc cctgcgcggc
     6901 ccgcatgcgg atctctggac gcatcagcgt tcagaaattc caagctggcg acctcctgag
     6961 taccgttgcc tgcgacctcg tcccgcgatc caccgtccag ccttacctcc gtaccgttct
     7021 cgccgaccat ggtgactagt tctgaagcga cctcgaagtt tatcgcaggc tgctggaagg
     7081 tctgccggtc cagttcggcc tggttcaact cggagttggc caggaactcg aggttgttca
     7141 tccgtaccac gaagtctgcc acgttctcgt agcggggcaa ggcgatgccc gtgaacgtga
     7201 ccgcacctgg tggatccagc gtctcctcca cgggggcgtc gatgtcgatg ggagcggcct
     7261 cggccgtgag ggtctgcagc gccgtgctct ctggtatgac aaaggcgaga ccctgaagga
     7321 actgatccca ggcgaaacga gtgcggtaga tgccgtctgc gacgggcttc ttctcttcga
     7381 ggcgcgtctt gaggtcccta tacgggctca actcggcgag gtgggcgttc tgctgcgcta
     7441 tctcgtcgtc cagttccgcg accctgtcct cgaggtcacc aagtctcata agcattatca
     7501 ggtacaaccc gaccatcacg atcaaagcga gcgcgcctgc gatcagcagt ataccgagga
     7561 ccccgccgcg gaacctttcg ccagggcgtc cccgctcctc gggtgggagg aggttgatcc
     7621 gcctcattcg tcctccatcg ccaggcccaa cgcaacggcg agcacaggct ccatcgcctt
     7681 tagctgctcg tccgagatgt tggagcggtt ggcggagagc ttctccacgg gatttccacg
     7741 gcccgtagga acgttcagca gttccccgag gtacctgtcg aggccgggga tcaaggccgc
     7801 ttctccggag ataagcaccc tggagacctc gcgggctact tcctgagacc tgtggtgttc
     7861 gatagaacgc tgcacctcat cggccagcgc ctggccagcg ctctcgaggc cgcgccgtac
     7921 gtcgaaggcg agcgccgggt cgaagccgtc ggactgcgtc tcatccgcca cgtcctcctc
     7981 atcgcccatc atgacccgcg agttgagcgc ctgcttctct gcttcatcct gtggaaggtt
     8041 agcgagctcc gcaactgctg ccacgaagtc tccgaggccg acgggcacga agcgcgtgag
     8101 tacgggattg ccgagatccg cgacgacgag gttggtgatc tccgctccga catcgagaag
     8161 cagtacggcg ccttcctcgc cgaagaaagg atcaggtagg gtagagcgtg tcagcgagag
     8221 cgccttgacg tcaacgccgg aaggccgcaa gccaccggtg cgaacggccg aagtgtagcg
     8281 tgagagcatc tccctctggg cggcgactac gaggaccctg tcgagatccg agcgctcctc
     8341 cctggggccg agcacgacgt agtcgagaac ggcttcatct agcggcatcg ggatgtggtc
     8401 ctgcgcctcg aagcttatgg cgccctgcag gtcgtcgggc tccatgtgcg gaaagtcgag
     8461 gagcctcacg actactttct ggttggagac gccgatagtg accgatttgc ccttgaacga
     8521 gtgcgagtcc cagaactctt ttatctccgc cgccagcagg tcgtggtcgg cgacctctcc
     8581 ttcgatgacg gtccctggcg gaagcctgtg ataaccgacg tgctggagga cgtaccctcc
     8641 ccctgaaccg gagacctgga cggctttgat cgacccggca tcgatgtcga tgccgatgtt
     8701 cttgcgcgaa ccaacccgtc tgaagcgtcg caaacgctcc atggcgccta gatccctgcc
     8761 agcctgagat agagggcgaa tatctcgggc ccctggaaca gagtcacgag gccgccgagg
     8821 gccataaaca ccccgaacgg caacgcgctc ctgcgacgta tcctcccgag cgccagaagt
     8881 atcacggcga cgaaggtacc gcacaatgct ccgaggaaga ccgccagcgc gccgtagggc
     8941 cctagaaagg ccccgagcat accgcccatc ttgacgtcgc ccatgcccat tccggccggg
     9001 tacgccagtg ccaggacgag caggccacct ccgatagcga aagcggagat cacgtatatc
     9061 caccagccgt cagggtccct gatcaccgag aggaccaatc ctaaggccgc acagggcagg
     9121 acgatcgcgt tcgggagcag gccgtgctcc aggtctatcg cggccaggga gatcagggcc
     9181 gagatcaaaa ccagcgccga gattagctgg atgctggctc cgaactggta accggctgcc
     9241 ccgaatagca aggccgtaag cgcttcgatc accgggtacc gcattgggat ccgctccccg
     9301 caactccggc atctgccgcg gagcagcagg tatgagacga gcggtatatt gtctttggga
     9361 gagatcggct ctccgcattg cggacagcgg gatgagggcc acaccacgga acgctttaga
     9421 ggcaccctgt ggataaccac gttaaggaag ctcccgacaa ccagtcccag aacggctgac
     9481 gccggtgcga tcaagcgccc tcccgggcaa cgtgtcgaag cgcgctcacc tccacgtcgg
     9541 ccccaacgga agctttgcgc ccgacgcttg agttgggacc cagaactact ctcgccccga
     9601 cccgcgcacc cgacccgaca accgcgccgg cggagatcca gctccccgcc cccacctcgg
     9661 cgtcgcgatc cacaacgcat gcaggtccca gaaaacagtg gctccccacc gccgcgtcag
     9721 gatgtacgac aacccccgaa gagaccacgc aaccaccctc gacggtggcg acatcggaaa
     9781 gataagcgag cggatgcacg accgtaaaga actgtccccc gagagccctc aaggaattgg
     9841 caacccgaag tcttgccccg ttgtcagtga tcgccacgac gatgtgaacg ggctcgacgg
     9901 agagcatgct cttgagcatc tctatgtccc caaggaccgg ataccctctc accttctccg
     9961 agatcgcagc gtgcgcatcg tcgtagaacc cgagcacccg gtctcccaac ccgctcgcca
    10021 atagaacgtc cagcgcgacc cttccgtagc cgcctgcccc cacgatggcg accctgatct
    10081 cttcctcgag acttatttcc tgtatctcga cttcgggttg aggtacgcct gtagtagttt
    10141 cgggctctct tatctctccc tcctcccaac agagattact ccagacgcct caggcagttt
    10201 agcaccctcg ctggcatctt gctcctatac caacatcgta ggtctagaag ctctttttgg
    10261 accctggatc aagggctgga gctaccgaaa cgctccttcg agggagttgc cgagaaggta
    10321 atctcgcgct gcaatgcgcc tgccacccgg taactgtagt tccaacacct cgagggtccc
    10381 gagcccacaa gagaccagta tggacccgtt ttcggctttg atacgaccaa tttccgtcga
    10441 tgaacgctct ttgtccagga ttctggaacg ccagatcttg accggcccat cgaccatagg
    10501 atggaacgtg cgcgccccta tgtgcggcgc cagggcacgg atcagatcgt gcacctcccc
    10561 aacctctcgc tcccacttta tgaccctgtc ttcatctaat actttagaag catatgtagc
    10621 ataaagactg tcctgttcga tgagggttaa ggcgtctctc tctactaaag ttagactctc
    10681 aactatagcg tcagctccga tatgggcgag gagatcggcg agttctcccc ctgtcatatc
    10741 cggtgatatc gggacgtggc gctgaatggc gattgggccc gtgtcgaggc cttcgtccat
    10801 ctgcatgatg gtcacgccgg tctcggtctc gccgttcatg atcgcgcgtt ccacgggggc
    10861 tgcgccgcgg tacttgggca gtagcgaggc gtgaacgttc aaggcgccgt tacgggcggc
    10921 gtagagtgtg tctgggcgca ggatctggcc gtaggcggcg accaccaggg cgtcgtattg
    10981 ggaaagttcg tcgcttattt cggagatgcg ggctggttgc cggagggtga ggccgtgaga
    11041 tgcggcgacc tcggcgaccg gggcctgtgt ggtcttgcgt ccccttccct tacgagggtc
    11101 gggttgcgag atcacgagcc ccacttcgtg gggagaggag atcaggcctc gcaggacggt
    11161 tgccgcgaag tccggggtac ctgcgaaggc taccttcaag gcgtgcgatc agccctgctg
    11221 ggcgagcagg cgctctcgcc actcgcgcat ggcggccttg cgtgattctc tgtctgtacg
    11281 gtccaggatc aagactccgt cgaggtggtc gacctcgtgc tggaggaccc tggcgagcag
    11341 gtcggtcgct tctatccgca gcggcttacc cgaggtgtcc tggcccgaga cggttacggc
    11401 gatcgggcgt tctacctcta cctggatacc cggtatcgag aggcaccctt ccggtcctgt
    11461 ctcggtcgtc tctgccgcgt cggtgaggac cgggttgacg atgacgtact catcgtcctc
    11521 gatcgcggcg acgagcacgc gcttgaggcg ccccacctgg ttggcagcga ggccaacccc
    11581 ctcgtactcg cgcatggtga tgagcatatc ctccgtaagg cgcagcagcg actcgtcgaa
    11641 gctctcgacc ggcgccgcgc gggttttgag cacagggtct ccgaacgtcc tgatttcgag
    11701 tgccacctct tctagacctc ctcgggatcc acctcaacgc gaaccttgag cccgtgcgtc
    11761 ctggctgcca tgcgggcagc aagggccgcc gcgcgggcca cggcgggccg ccccctggac
    11821 cggagcagga cccgccacac tgtcgttccc ccggtgcgcc tgagtggaac cggggccgac
    11881 atctcgaccc caggctcgag agccggccgc acacgggatt ttaccgtaac agagaccact
    11941 tcctcagacc cttcgagatc caggtaggcg acgtgagcga acggagggta gccgagcgtg
    12001 cgcaatcgtg aaagctcggc ggccgcgaac ttctcgtagt cgccccgtac tcccgcgcgc
    12061 agcgcgtagt gttcggggag atgagtctgg atgaggagca gctctttggc cgcctcagca
    12121 gcgccgtaga gtaacctgaa tgatttctcg accgcaccga tgttgttacc cgcaagaaac
    12181 gcgtcgatat cggggaggat cacggcgtcc cactcgctgt ccagtattcg gggtgcagtt
    12241 cccactacca ccgaggcgtc agcatggtct tgctcgtcgg cggtgatcag acctacccgt
    12301 tctccgagga gttccgagag ttcatcgcgc acccgctcga cgccgagtcc cgtcggtctc
    12361 actcgggacg aaccgcactg ggtgcatggg cccgccttct cccgatagcc gcaacggctg
    12421 cagatcaaag gtccacctct tccgtgcatg acgagcggga ggtcgcattt cgggcaacgc
    12481 tttacagccc cacaatggct acatgagacc gctgtcgcgt aaccgagccg cttgagaatg
    12541 aggccaacct ttcccccttc gtccacgcac ctgcggcacg cgtcgatcag ggtggagcta
    12601 aaagtcgcgc ccgagccccg catgtctacg atacgcaccg ccggccaccg cacggacctg
    12661 ctcgcaggaa gctctcgaat gcgctccctt ttgcggactt ccacggcgta tagcctgaga
    12721 gaagggaacg gtgagaggaa gaataccgcg gcccgctcca ggcgggagcg ttcgagcgcc
    12781 acgtcccgca cgtgcagagg tagtccctcg tggccgggcc cggccctatg ggcttcgttc
    12841 ggttcgtcca cgacgcagat agaacccgga cgatccagtg ccagcagcgc cgccgtacgg
    12901 gtccctacga cgacgtctac ctctcccgcg cgggcggcct cgtagacggc agccctgcca
    12961 cgacccaggc cgccgtggta tgcggcgacg gtgtgtcctg cgggaagctc acggcgaagg
    13021 gtatccacca tacgctcgac gacggcgatc tccggcgcca ggatcagggc ttgatcccca
    13081 cgttccagcg tagcctttgc tatggtagtc accgcgtcca actcctcgct cctggccgtc
    13141 ctccagagga aagtcccgcc gctatccacg gcccgccctg cgtttcgtgt gaacgacgag
    13201 aggtgttccg gcacggcacc gtcgcccatg gtggcaagca gttgcgccgg cccaggacgg
    13261 cgtactagcc gcaccgcgcc tctgcggacg agctcccgca gagcacttcg gctcgccccc
    13321 gtctcggaga gcaagg
//
LOCUS       DATAIR010000271         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_432991, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000271 DATAIR010000000
VERSION     DATAIR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432991"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VHM69_06625"
     CDS             complement(<1..290)
                     /locus_tag="VHM69_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2740103.1"
                     /translation="MTTAGDKQNPLQSDRGTTTIQDAVVLALASVAAQEVEHVHLSHG
                     GTRLPGDNSPTVGEFLGNVTGGMGRSRGISVEVGEQQTAVDLTLNVDYGQPIA"
     regulatory      520..597
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            733..1503
                     /locus_tag="VHM69_06630"
     CDS             733..1503
                     /locus_tag="VHM69_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740104.1"
                     /translation="MRARSGIEHNEVVHLVEAYRERDDRAAIERILSIHGRLLNHIVR
                     RHAASSGETYEDLLQVGYVGLIKAVNGYRIDSDARFDSYAYSMIEGEIRHHLRDTGLV
                     KKPRWARSLYAKVSEATSRLTAELGRPPLVEEVAGDVNVSEKGVLELMKLYLDTNVSS
                     LDEGEEGVDVSAIRSLHHESFSLPIEDRIQLEEAFESLTELQRKVIYLFFYKDLSQTE
                     IGRMLGLPQRKISRVVASGIKTMKDSQDAEGKGLGWRN"
     gene            1491..2015
                     /locus_tag="VHM69_06635"
     CDS             1491..2015
                     /locus_tag="VHM69_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2740105.1"
                     /translation="MAQLKDIPRSERGFTLPEVMMTIVVLGILLTIASSTWFRVIESR
                     TVDSAANQLAADLRLAHTRSSNELTDWRVQIFTNRGDPGQTDYKVMRPSDGFTSNRAL
                     PEDSMVSSTGTELNESGGSRTFRFQSTGAVEAVGGFGDSDGDGEIRIAVTVDGDPIRK
                     LTVVPATSRVRIVP"
     gene            2005..2487
                     /locus_tag="VHM69_06640"
     CDS             2005..2487
                     /locus_tag="VHM69_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2740106.1"
                     /translation="MFRKLLRDEGGYSLVEVIVSIMILAIAILPMVSMFDAGLRAAAV
                     GSNYDTARALANEKLEEIKALSFRDPDSPAGAPANSILEKYKPVNEPSTAAGGLGPGS
                     PVTGTKGIFTYTVTTRYVQQDFNTTPATPIRTSFVKVEVKVSWQGNSITVTSVRVVNV
                     "
     gene            2499..3110
                     /locus_tag="VHM69_06645"
     CDS             2499..3110
                     /locus_tag="VHM69_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2740107.1"
                     /translation="MREEGGFTLAEVMVTMVIMVTVMFALYSIFDMSLRVYGVGNDKV
                     EATQNARIGLDKIARELRAAYPASKAGGKTQLFWAPGACLTAVMPSQTQITFGNDSNG
                     NRMICNATTGLMDAGEEITYSVSGSTLQRNGQPVVEFLQDLDGDGKALTFTYMDAKGN
                     TTSNEGAIARVHVKLEVAVDRGIHEEPVKQTLETDVALRNRKP"
     gene            3125..4525
                     /locus_tag="VHM69_06650"
     CDS             3125..4525
                     /locus_tag="VHM69_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740108.1"
                     /translation="MRDESGMTMAIAIMMIVIVGVMGAGLLTFVNRDLESVITVNRGQ
                     RALELADAGAQAAKQQLRADPDRSHYDADTSNNVAWSYVVPSGQPGVTRSLAGGTTRV
                     TIQTLQNSTIDAHLTDPNYAPELFNVLAPKDYYKVTSDATVGNVRRKVEAIFVSSQAQ
                     FPKAYLATMDINIGSQSTRINNVSMYAGRDINGLYLNNLQGEDIAYGNWQNAYNDKAR
                     PGNGVTPMPAGAAAERSITYSPTSENVTQKDPPAAPTDRFRKLDFDNSSSTVPGYKFC
                     RKGVTKNPDGTICWPSGSPQPANVITYPFEANTRDINLDLLKELAVQQGHYFNPSGGT
                     THTINDTTYPLSASSKQTIYYVEFTGSTRGSVDYSASGTREGTIVVINGDFKTSASAD
                     GFTGVVIMRDPNNFAATANPLQYTNQGDFTFTGYLNIEGSATIKGSANPILTQDVLRR
                     PGFFDVTLWSWRERYQ"
     gene            4621..5076
                     /locus_tag="VHM69_06655"
     CDS             4621..5076
                     /locus_tag="VHM69_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2740109.1"
                     /translation="MTVRKQPTTGGTLSMLHFFAKRLNELHKVKREERGFTLIELLVV
                     IIIIGILAAIAIPAFLAQRERAQVAAVESDLRNAGTAYTQCITDKSDFAQCNDQTKLE
                     AYGYNKSADVTFVSVTGTADAAVITAKHTDNPAVTGTYKSDTGQVSVTS"
     gene            5154..5300
                     /locus_tag="VHM69_06660"
     CDS             5154..5300
                     /locus_tag="VHM69_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740110.1"
                     /translation="MKVDKGRGEDTAARDASTRRLGVWAAGVIFALVGDNSAFLCGVS
                     HGFR"
     regulatory      5325..5404
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5630..6085
                     /locus_tag="VHM69_06665"
     CDS             5630..6085
                     /locus_tag="VHM69_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740111.1"
                     /translation="MSNGPNTEPINILLVEDNPGDARLAVEALRDSKVHNKLYHVEDG
                     VEAMQFLRHRVKNDHVPPPDLILLDLNLPKKDGREVLAEIKEDPELRLVPVVVLTTSA
                     AERDLLKSYDLHANAYIVKPIDLDQFIDVVQAIESFWFSIVKLPPPPDD"
     gene            6095..>6879
                     /locus_tag="VHM69_06670"
     CDS             6095..>6879
                     /locus_tag="VHM69_06670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2740112.1"
                     /translation="MTAVGVARRAPVRVLLVEDNPGDARLVEILLSGTGQDFDVKHVG
                     TLGEALDELDQPFGVVLLDLSLPDSAGLQTVERMRRAAPELPLVVLSGRDDEEIALQA
                     LQGGAEDYLVKGQGDGDLISRSIRYSIERKIAEEKLARLAQFDPLTGLANRALFHDRL
                     EHAVARVERHGGMLALLFLDLDRFKAVNDTVGHIGGDALLTQVARRIDARVRESDTVA
                     RLGGDEFAIIIEDLVEAREAARVAEDLIDIISEPFVVNGHEIP"
BASE COUNT         1503 a         2029 c         2153 g         1194 t
ORIGIN      
        1 gcgatcggct gaccgtagtc cacgtttagc gtcaggtcaa cggcggtctg ctgctcgccg
       61 acctcgacgg agatgcccct gctgcggccc atcccgcctg tgacgttgcc gaggaattct
      121 ccgacggtag gggagttgtc accaggcagc ctggtgcccc cgtgagagag atggacgtgt
      181 tcgacctcct gggccgccac actggcgagg gcgagcacca ccgcgtcttg tatggtcgtg
      241 gtcccccgat cgctctgaag cgggttctgc ttgtctccgg ctgtcgtcac ggcgcttccc
      301 ctttcgtcct ttttccgtga taccgtagaa tatcttatct ggcaccttgc ggcgcgtagc
      361 gcgccttctc gcgctgagga gcaccagcaa agcatggtgt tgcaagatcg acggaggggc
      421 tggtatcgcg cacgcttcaa tgaggaacgg gcacggggaa aacaggcaac aaaccattat
      481 ggttgcttag atctgcaaat gggtatatat ttgtgccagt ccacgaaaag gcaaacccgc
      541 cgtgaggcgg ggacgcaaag ccagcggacc tcgtgcagag gtggccgggc tgccgacggg
      601 aggttcacca cttgggaatc ataggcaggt ggagagtcta catccctcca gtgcccgttt
      661 actctgggta cgacagaccc caaaatatgt tggccacggt ggccgtatgc catatggatg
      721 tcttgcggat cgatgcgagc taggtcgggc atagagcaca acgaggtcgt tcatctcgtc
      781 gaggcgtaca gggagcggga cgaccgggcg gcgatcgagc gcatcctctc catccacggc
      841 aggctgctca accacatcgt gcgcaggcac gccgcttcct cgggtgagac ctacgaggac
      901 ctcctccagg taggttacgt cgggctcata aaggcggtca acggctacag gatcgattcc
      961 gatgccaggt tcgactcata cgcctactcg atgatagagg gtgagatccg tcaccacctg
     1021 agggacacgg ggctcgtgaa gaagccgcgt tgggcgcgca gcctctacgc gaaggtctca
     1081 gaggcgacga gcaggctcac cgccgagctg ggcaggccgc cgctggtcga agaggtagcg
     1141 ggggacgtga acgtctcaga gaagggtgtc ctcgagctga tgaagctcta tctcgacacc
     1201 aacgtctcgt cgctcgacga gggcgaagaa ggagtcgacg tttcggcgat ccggagtttg
     1261 caccacgaga gcttctcgct gcccatcgaa gaccgcatac aactcgaaga ggctttcgag
     1321 tcgttgacgg aattacagcg caaggtcatc tacctcttct tctacaagga cctctcccag
     1381 accgagatag gcaggatgct gggactcccg cagcgtaaga tctcacgcgt tgtggcttcg
     1441 gggatcaaga ccatgaagga ctcccaggac gcggaaggca agggtcttgg gtggcgcaac
     1501 tgaaggatat cccgaggagc gaacgcggct tcacgctgcc cgaggtgatg atgactatag
     1561 tcgtcctggg tatcttgctc accatagcat catcgacctg gttcagagtc atcgagagcc
     1621 gcacggttga ctcggccgcc aaccagctcg cagcggatct gcgtctggcg cacacccggt
     1681 ccagcaacga actaaccgac tggcgggtgc agatcttcac caaccgtgga gacccaggtc
     1741 agacggatta caaggtgatg cgaccgagcg acggcttcac ctccaaccgg gccctgccgg
     1801 aggacagcat ggtgagcagt acaggcaccg agctcaacga gtcaggcggc agccggacct
     1861 tcagattcca atcgacggga gctgtggaag ccgtgggcgg attcggcgac agcgacggtg
     1921 acggagagat ccgcatagca gtcaccgtag atggagaccc gatccggaaa ctgaccgtgg
     1981 tgccagcgac ttcgagggtg cgcattgttc cgtaagctcc tgagagacga gggcggctac
     2041 tcactggtgg aggtcatagt ctccattatg atcctcgcca tcgccatcct tccgatggtg
     2101 agcatgttcg acgcgggcct gcgtgccgcg gccgtgggca gcaactacga tacggcgcgg
     2161 gcgctggcca acgagaagct ggaggagatc aaagcgctgt ctttcaggga tcccgacagt
     2221 cccgctggtg ctcccgcgaa cagcatactc gagaagtaca agcccgttaa cgaaccatcc
     2281 actgcggcag gaggactggg accgggttcg ccggtaaccg gtacgaaggg gatcttcact
     2341 tacaccgtca cgacgaggta cgtgcagcag gacttcaata cgacccctgc gaccccgata
     2401 cgaacgtcgt tcgtcaaggt ggaggtgaag gtgagctggc agggcaacag catcacggtc
     2461 accagcgtca gggtggttaa cgtatgaagt ggcgaatcat gagagaagag gggggattta
     2521 ccctcgccga agtcatggtg actatggtga tcatggtcac cgtcatgttc gccctttaca
     2581 gcatcttcga catgagcctc agggtctacg gcgtcggcaa cgacaaggtc gaggccacgc
     2641 agaacgcccg cataggactg gacaaaatcg ctcgggagct tcgggccgcc taccccgcga
     2701 gcaaagccgg cggcaagacg cagctgttct gggctcctgg cgcctgcttg acggcggtca
     2761 tgccgagcca gacccagatc accttcggga acgactctaa cgggaaccgc atgatctgta
     2821 acgcgaccac ggggctcatg gacgccggag aagagatcac ctacagcgtg agcggaagca
     2881 cactccagag gaacggccag ccggtggtcg agtttttgca ggacctggac ggtgacggca
     2941 aggcgctgac gttcacatac atggacgcca agggcaacac gacgtccaac gagggcgcca
     3001 tcgcgagggt acacgtaaag ctggaggtgg cggtggaccg tgggatccac gaagagccag
     3061 taaagcagac cctggagacc gacgtggccc tcaggaaccg gaagccgtag agatggaccg
     3121 gttcatgaga gacgagtcgg ggatgacaat ggcgatcgcg atcatgatga tcgtcatcgt
     3181 gggggtcatg ggcgcagggc ttcttacctt cgtgaacagg gacctcgagt ccgtgataac
     3241 ggtgaaccgg ggacagaggg cgctcgagct ggccgacgcg ggggcccagg ccgccaagca
     3301 gcagttgcgc gcagatccgg acaggtcgca ctacgacgcc gacacctcca acaacgtcgc
     3361 ctggtcgtac gtagtgccat ccggtcagcc tggtgttacc cgcagcctgg caggcgggac
     3421 gacgagggtt acgatacaga ccctccagaa ctcgacgatc gacgcccact tgacggaccc
     3481 gaattacgcg cccgagctct tcaacgtcct cgccccgaag gactactaca aggtgacctc
     3541 cgacgcgacc gtgggcaacg tcaggcgcaa ggtcgaggcc atctttgtct cgtcgcaggc
     3601 ccagtttccg aaggcatacc tcgctaccat ggacatcaac atcggcagcc agtcgaccag
     3661 gatcaacaac gtcagcatgt acgccgggcg cgatataaac ggcctgtatc tcaacaacct
     3721 ccagggcgag gacattgcat acggcaactg gcagaacgcg tacaacgaca aggccagacc
     3781 gggcaacggg gtaacaccga tgcccgcggg agccgccgca gaacgctcca taacctactc
     3841 gcccacgagt gagaacgtca cccagaaaga tccgcccgcg gcgcctaccg accgcttcag
     3901 aaagctggac ttcgacaaca gctcctccac cgtgccaggc tacaagttct gcagaaaagg
     3961 cgttacgaag aaccctgacg gtacgatctg ctggccgtca gggagcccgc agcccgccaa
     4021 cgtaattaca taccctttcg aggccaacac gcgggatata aaccttgacc ttctcaagga
     4081 gctggccgtt cagcagggac actacttcaa cccgtccggc gggacgactc acacgataaa
     4141 cgacacgacg tacccgcttt cggcatcttc gaagcagaca atctactacg tggagttcac
     4201 gggaagtaca aggggcagtg tggactactc ggcatccggc acgagggagg ggaccatagt
     4261 agtcataaac ggcgacttca agaccagtgc ttcggctgac ggctttacgg gggtcgttat
     4321 catgcgagat cccaacaact tcgcggccac ggctaacccc ttgcagtaca ccaatcaggg
     4381 tgacttcacc tttaccggct acctgaacat cgagggtagc gcgacgatca agggtagcgc
     4441 gaacccgata ctcacgcagg acgtgctgag acgccccggc ttcttcgacg tgaccctctg
     4501 gagctggcgg gagcggtacc agtagcaaaa ctcttccaca cggccactgg ccggccatct
     4561 ccaacatttt gcagaatatt ctctcctaaa gtgtctaatt tcggtgatag tgggcctaac
     4621 ttgactgtaa ggaagcaacc aaccacagga ggaacactgt ccatgctaca tttttttgcg
     4681 aagaggctca atgagcttca caaggtaaag agggaggagc ggggcttcac cctgatcgag
     4741 ctgctggtcg tcatcatcat catcggcatc ctcgccgcca tcgcgatccc ggcgttcctc
     4801 gcacagcggg agcgggcgca ggtagcggcg gtcgagagcg atctgcgaaa cgccggaacc
     4861 gcttacaccc agtgcatcac ggacaagagc gatttcgccc agtgtaatga ccagacaaag
     4921 ctcgaggcct atggctacaa caagagcgca gacgtcacct ttgttagcgt taccgggact
     4981 gcagacgcgg ctgtgataac ggcaaagcac accgacaatc ccgcagtaac aggcacgtac
     5041 aagagcgata ccggacaggt cagcgtaacc tcgtagccct gagcagcgag tagtaaagtg
     5101 gcaggccccg cgctccagag cgcggggcct gttttcgagt cgagggacgg gacatgaagg
     5161 tcgataaagg ccggggtgaa gatacggcgg cgcgggacgc gtcaacgaga cggctcgggg
     5221 tgtgggcggc gggggtgatc ttcgcgctcg tgggcgataa ttccgccttt ctgtgtggcg
     5281 tgagtcacgg ttttcgctga aaggcgcatg gtatacaatg atgcctacga caaaggcaaa
     5341 cccgccgcga ggtgggggcg caaagccacg ggtcttacga aaggaagata gccgggttgc
     5401 cgaatggaag gtcgatctcc atagttccac cgttcgatac gcgtggggtg aaggacgggg
     5461 cagcaagaat agcctggtct cggacctgcg ttcgcgaagc tcttatcaag catggtcgcg
     5521 gttggcggca aacctccacc agatcgctcg tcttgagcga gggccgcgct tttgggggct
     5581 cgcatctaat tcgtgaagat cctgaagtcc gatcggggag gggattacgg tgagtaatgg
     5641 accgaacact gagcccatca acatcctcct tgtggaggac aaccctggcg acgcgcgcct
     5701 ggcggtcgag gcgctcaggg atagcaaggt ccacaacaag ctctaccacg tcgaggacgg
     5761 cgtcgaggcc atgcagttct tgcgccaccg ggtcaagaac gaccacgtgc cgccgccgga
     5821 tctgatcctg ctcgacctca accttccgaa gaaggacggt cgcgaggtgt tggctgagat
     5881 aaaggaggac cccgagctca ggctcgtccc tgtggtcgtc ctcacgacct cggctgcgga
     5941 gcgggacctc ctcaagagct acgacctgca cgcgaacgcc tacatcgtca agcccataga
     6001 cctggaccag ttcatcgatg tcgtacaggc catcgagagc ttctggttct ccatcgtgaa
     6061 gctgcctccg ccgcccgatg actgagggga ccccgtgaca gcggtcggcg tggcgcggcg
     6121 cgctcccgta agggtgttgc ttgtggagga caacccaggc gacgctcggc tcgtcgagat
     6181 tctgctctcc gggaccggcc aggacttcga cgtgaaacac gttggcactt taggcgaggc
     6241 gctcgacgag ttggaccaac ccttcggcgt cgtgctcctc gacctctcgc tacccgattc
     6301 agcgggactc cagaccgtcg agcggatgcg gagggccgcg cctgaacttc cgctcgtggt
     6361 cctcagcgga agggacgacg aggagatcgc cctgcaggcg ttgcagggcg gcgcggagga
     6421 ttacctcgtc aaaggacagg gagatgggga cctgatctcc cgctccatac gttactcgat
     6481 cgagcgtaaa atcgccgagg agaagctggc ccgactggcg cagttcgatc ccctgacggg
     6541 tctggccaac cgcgcactct tccacgaccg cctggagcac gcggtcgcca gggtcgagcg
     6601 ccacggagga atgctcgcgc tcctcttcct cgacctcgac cggttcaagg ccgtgaacga
     6661 caccgtgggg cacatcggcg gggacgcgtt gctgactcag gtagcacggc gtatcgacgc
     6721 gcgggtgcgt gagagcgaca ccgtcgcccg cctgggaggt gacgagttcg cgatcatcat
     6781 cgaggacctt gtcgaagcaa gggaggccgc gcgggtcgcg gaggacctca tagacattat
     6841 ctccgagcct ttcgtcgtca acggccacga gataccgat
//
LOCUS       DATAIR010000272         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_295009, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000272 DATAIR010000000
VERSION     DATAIR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295009"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VHM69_06675"
     CDS             complement(<1..725)
                     /locus_tag="VHM69_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740113.1"
                     /translation="MALAIAIFVMVLVGVMGAGLLVFVRNDLESVIGVNQGQRAFAIA
                     EAGIQIAEVQQLSDVARRHYDRDRTNDCTNGQIRATAVDWSPNTTIYSDPRNCGSVTT
                     TRSAGGVTRDFAGGKFNVTIECFDQVGDASDICSGISEMAPENVEASRKAFFKITSTG
                     YYPADGSGAKRKVEAIYYTNSLDVPTAYYTPKDIEFNGSPTVSGVSFFAGGNIKVGNA
                     DINPTVPALYRDWDTTNPANFSST"
     gene            complement(758..1357)
                     /locus_tag="VHM69_06680"
     CDS             complement(758..1357)
                     /locus_tag="VHM69_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740114.1"
                     /translation="MRTIRVQVRRLLREESGFSLAELLVTMLMMLLVMFALYSIFDMS
                     LRVFSFGNDKTEAVENARLGLERMERELRGAYPYERSAGNLTLFPGFAANPSNSITFG
                     NDLDADRQIQSDTSTEQVAYSLSGDEILRNGKPLVEFVQPDGLTFTYCKTLTSCPSSI
                     SESEIKLVRIDLAVSVDRGTGNPATQTLSTDVTLRNRGG"
BASE COUNT          287 a          455 c          430 g          341 t
ORIGIN      
        1 gtggagctga agttggctgg gttggtagta tcccagtctc tgtagagtgc gggtactgtt
       61 gggttgatgt ccgcgttgcc gaccttgatg ttgcccccag cgaagaagct gacgccagat
      121 acggtggggc tcccgttgaa ctctatatcc ttgggggtat agtacgccgt cggtacgtcc
      181 agtgagttgg tgtaatagat agcttcgacc ttgcgcttcg cgccgctgcc gtccgcaggg
      241 tagtagccgg tggaggtgat cttgaagaat gctttcctgc ttgcttcgac gttctccggc
      301 gccatctcgc tgatcccgct gcagatgtcg gaagcatcac cgacttgatc gaagcattct
      361 atggtaacgt tgaacttgcc tcctgcgaag tctctggtaa ctccaccggc ggaccgcgta
      421 gtcgtcacgc taccacagtt tctcggatcg ctatagatcg tcgtgttcgg agaccagtcc
      481 actgccgtgg ctcgtatctg cccgttggtg caatcgttgg tacgatccct atcgtagtgc
      541 ctgcgggcga cgtcggagag ctgctgtacc tcagcaatct gtatcccggc ttcagctatc
      601 gcgaacgccc tttgcccttg atttaccccg attacggatt ctaggtcatt ccgcacgaag
      661 acgagaagtc ccgcccccat cacaccgacc agcaccatca cgaagatcgc tatggcgagc
      721 gccatccccg attcctcctt agacagtcgc ctgagcacta gcctccccga ttcctcaggg
      781 taacgtcggt ggaaagtgtc tgagtcgccg ggtttccggt tccgcggtcc acgctaaccg
      841 cgaggtcgat tcgcactaac tttatctcac tctctgagat cgaggaggga caactggtaa
      901 gagtcttgca gtaggtgaac gtgagaccgt caggctggac gaactcgaca agcggtttgc
      961 cgttccgcag aatctcgtcg ccgctcaggc tgtacgcaac ctgttccgtt gacgtatctg
     1021 actgtatttg gcggtctgcg tcaaggtcgt tgccgaaggt gatcgagttc gaagggttgg
     1081 cggcgaagcc ggggaagagc gtgagattgc ctgcggacct ctcataagga taggcccccc
     1141 tgagctcccg ctccatcctc tccaggccca accgcgcgtt ctcgaccgcc tcggtcttgt
     1201 cgttaccgaa ggagaaaacc cggaggctca tgtcgaagat cgaatagagg gcgaacatga
     1261 ccaggagcat catcagcata gtgacgagca gttcagccag gctgaagccg ctctcctcac
     1321 gcagcagccg ccgtacctgt acacggattg tcctcacttc ccgatcaaac ccgtcgtggt
     1381 aagcggcccg ccggaaggcc agctcaccgt caccgtgacc tgcgccagcc tcgtctcgta
     1441 ggccgcttcc tcgaccgcgc tcgggccaag acgcatgtac tcggtccgaa cctgacaggt
     1501 gaattgcgac cct
//
LOCUS       DATAIR010000273         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110101, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000273 DATAIR010000000
VERSION     DATAIR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110101"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VHM69_06685"
     CDS             <1..1007
                     /locus_tag="VHM69_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX2740115.1"
                     /translation="RDSGSVASALITLGIVLALFLCVLLHEFGHSLVAIRLGSEVQDI
                     TLLPIGGLARMKSLPERPIDEVKIAVAGPLVNVVLAPIFYGLALLFGGNLSVPTDILS
                     GAGSLGQVFAALGTINVALVVFNMIPAFPMDGGRVLRGLLATRLGQVRATDISSTIGQ
                     AFAIIFILLGFFGNNFLLAIVGFFVFLGASGEAQLVRQKEQMRGLAVADVMGSKGRTE
                     TVTPYHNFGQILDSVIHGYQMDFPVVDEEERLVGIITRNEIMSAAHSPARFSSVHDLM
                     KTDFPTISPDADLFTEGQRVLRESGLRALPVVKDGNLVGMLTIEDVGQAALLRDLRKR
                     "
     gene            1014..1922
                     /locus_tag="VHM69_06690"
     CDS             1014..1922
                     /locus_tag="VHM69_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX2740116.1"
                     /translation="MRIASLLPSATELVHFAGGTLVGVTHECDFPPGVEELPKLTSSK
                     IESHSMTSREIDAAVGALTDEGSIYALDAELLERLAPDLIITQGLCEVCAVSMNLVET
                     ATSQMSEVPEILSLNPTSLGDVLGDAVGIGEALGHGEETRAKVAALKERLASVQDAVA
                     GLSRPRVGCIEWLDPPFSAGHWVPEMVRLAGGEEVFAGPGEPSARLTWDEVFEAAPEV
                     LILMPCGFDAERTIKEALVLPELPDWGELPAVKIGRVWAVDANSYFSRPAPRLVDGVE
                     ILARILHPEAFPDAPENALRLTNDSW"
     gene            2022..>2539
                     /locus_tag="VHM69_06695"
     CDS             2022..>2539
                     /locus_tag="VHM69_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2740117.1"
                     /translation="MEEIEALVARVGTHPVYGYAHCQRVYALAEDLASSEGVSYDAEI
                     LRVAALLHDIGLYKAYALREPPDHAKRSAAVARRILQDWDFPPQASQAVIEAIERHPP
                     GASTGTASETILLKDAVGLDYLGAIGISRMLAMVGTEDDVPDIPTAIWNAESLRQRIP
                     DLLILESSKELAR"
BASE COUNT          441 a          869 c          770 g          459 t
ORIGIN      
        1 acagggattc tggcagcgtc gccagcgcgc tcatcaccct gggcatcgtc ttggcgctgt
       61 tcctctgcgt cctgcttcac gagttcggcc actcgctcgt tgccatacgt ctcgggagcg
      121 aggtccagga cataacgctc ctgcccatcg gcgggctcgc caggatgaaa tcgttgcccg
      181 agaggcccat cgacgaggtg aagatcgccg tagcaggtcc gcttgtaaac gtcgttctcg
      241 caccgatctt ctacgggctt gccctgctct tcgggggcaa cctctccgtt ccgacagaca
      301 tactctctgg ggcaggttcc ctggggcagg tcttcgccgc gcttggcacc atcaacgtcg
      361 ccctcgtcgt cttcaacatg atccccgcct tccccatgga cggtgggcgc gtgctgcgcg
      421 gcctgctcgc cacccgcctg gggcaggtgc gcgccacgga tatctcatcg actataggac
      481 aggccttcgc catcatcttc atcctcctgg ggtttttcgg caacaacttc ctgctcgcca
      541 tcgtcggctt cttcgtcttc ctcggcgcca gcggcgaggc ccaactggtc cgccagaagg
      601 agcagatgcg cgggctcgcc gtagctgacg tcatgggttc caagggcaga accgagaccg
      661 tgacgccgta tcacaacttc ggccagatcc tcgactccgt catccacggc taccagatgg
      721 acttccccgt cgtcgatgag gaagaaaggc tcgtcggcat catcacccgc aatgagatca
      781 tgtccgcggc ccattcccct gcccgtttct ccagcgtcca cgacctgatg aagactgatt
      841 tcccaacgat ttcgcccgac gcggacctct tcacggaggg ccagcgcgtc ctgcgggaga
      901 gcggcctgag ggccctgccc gtcgtgaagg atggcaacct ggtcgggatg cttacgatag
      961 aagacgttgg ccaggccgcc ctgctccgcg acctccgcaa gcggtagggc agcttgcgca
     1021 tagcttccct gctccccagc gcaaccgagc tggtccactt cgccggaggt accctcgtcg
     1081 gcgtcaccca cgagtgcgac ttcccgcccg gcgtcgagga gctcccgaag ctcacgagca
     1141 gcaaaataga aagccactcc atgaccagcc gcgagataga cgccgccgtc ggcgccctga
     1201 cagacgaggg cagcatctac gccctcgacg cggaactcct agaaagactc gcccccgacc
     1261 tcatcatcac ccagggtctg tgcgaggtct gcgccgtctc catgaacctc gtcgaaacgg
     1321 cgacctcaca gatgagcgag gtgcccgaga tactctcgct caacccgacc tcgctcgggg
     1381 acgtcctcgg cgacgcggtc gggataggag aagccctcgg ccacggagaa gagacccgcg
     1441 cgaaggtcgc cgcgttaaag gagaggctcg ccagcgtgca agacgcggtc gccggtcttt
     1501 cccgtcctcg cgtcgggtgc attgagtggt tggatccgcc gttctccgcc ggtcactggg
     1561 ttcccgagat ggtgcgcctc gcgggcgggg aggaggtctt cgccggtccc ggcgagcctt
     1621 ccgcccggct aacctgggac gaggtattcg aggccgcgcc tgaggtcctg atcctcatgc
     1681 cctgcggctt cgacgcggaa cggacgataa aggaggcgct tgtcctgccc gagctccccg
     1741 attggggcga gcttccagca gtaaagatcg ggagggtgtg ggccgttgac gccaactcgt
     1801 acttctcccg tcccgccccc cgcttagtgg atggggtcga gatcctggcc cgcatactcc
     1861 atcccgaggc attccctgac gcgccggaga acgccttgcg tctgactaac gactcctggt
     1921 aagaggtagt accccaggtc gagggtcgta gaggttctgg cgaagttact ctcgcaccgg
     1981 cgagatctat tcgctagact tccactgtga tgaagccgct tgtggaggag atagaggcgc
     2041 tcgtcgcgcg ggtggggacg catccggtct acggctacgc ccactgtcag cgggtctacg
     2101 cccttgcgga ggatctggcg tcgtcggaag gtgtctccta tgacgcggag atactgcgcg
     2161 tcgcggccct cctgcatgac atcgggctct acaaggccta cgccctgcgg gagccgcccg
     2221 accacgccaa acgctctgcc gcggtcgccc gccgcatctt acaggactgg gatttcccac
     2281 cgcaggcgag ccaggcggtc atagaggcga tcgaacgcca cccgccgggc gcctccacgg
     2341 gcactgcatc cgagaccatc ctcctgaagg acgcggtcgg cctcgattac ctcggcgcca
     2401 taggcatctc ccgcatgctc gccatggtcg gcacggaaga cgacgtgcca gatataccga
     2461 cggcgatctg gaacgcagag agcctcaggc agcgaatccc cgacctcctc atcctcgaga
     2521 gcagcaagga actggcccg
//
LOCUS       DATAIR010000274         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_17859, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000274 DATAIR010000000
VERSION     DATAIR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17859"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VHM69_06700"
     CDS             <1..608
                     /locus_tag="VHM69_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX2740118.1"
                     /translation="DTTEMIHQSMRHAASSVLGRDDIPRETLLANVGQPLPRQMELID
                     AERAELLLEAYRSHHEEHHDDLIGEFPGIEESLSRLHSAGIKVAVVTSKRRVSVEMAL
                     QNYPGLRNVVDRFVTMEDTTEHKPDPEPLLRGLELLGNVPKEEAVYVGDSPFDVEAAK
                     AAGLRSVAVSWGAFSEDTLRAAGPDHLVPDIDAVVDVLLTL"
     gene            complement(628..1743)
                     /locus_tag="VHM69_06705"
     CDS             complement(628..1743)
                     /locus_tag="VHM69_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2740119.1"
                     /translation="MFPALADNDFAYLNSGSSGPPPHYVLEAMRDADDLCSGPAYLEG
                     VGLIARQAAYSSRAREAAASLVGVRPDDVALTLNTTHGMNLGVESIEWREGDEVVSVT
                     TEHPGCLVPLHNLRKRFGVKVNLVSPTVTPERIQAALTHKTRLIALSHVDWTNGEILP
                     LGEICGLARERGVLTLIDGAQSVGNIQVDAPDTGADMYAFTGHKWVLGPEGMGAFYVR
                     PGLPVHSPNVGFMSLHTPTDFDAEGDYELRSDARRFEASTMSPTLAGGLAAAAEAAHE
                     RGEAGFEGIRHRADLLMDLLSTLPRVTLRSPKPAQSGLVSFDIEGVTSKDAADWLSQQ
                     RFILRYIPGPNSCVRASTHLFNSEEELEALAKSVGRL"
     gene            1795..>2361
                     /locus_tag="VHM69_06710"
     CDS             1795..>2361
                     /locus_tag="VHM69_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2740120.1"
                     /translation="MAVLYNSPFMQNGNLVRIGRRRRRIRNKSKLWQRLRTAFLLLCL
                     VAILGTVGFVASAVSTYQTLAANMPDLDNYHSTELAQTSLIYDTNGNVVDQLYGVQNR
                     FVVPLDQVDPTLQKAVISIEDHRFYQHRGLDFEAIGRAAVENVQSLSIQEGGSTLTQQ
                     LIKNTYIAQQERYIPSFERKFVEASLAWQ"
BASE COUNT          432 a          773 c          730 g          426 t
ORIGIN      
        1 tcgacacgac ggagatgatc caccagtcca tgcgccacgc cgcgagctcc gtactcgggc
       61 gcgacgacat cccccgcgag acgctgctcg ccaacgtcgg ccagcccctc ccgcgccaga
      121 tggagctgat cgacgccgaa agggccgagt tgctcctcga agcctaccgc agccaccacg
      181 aggaacacca cgacgacctg atcggggagt tcccaggtat cgaggagtcg ctttcacgcc
      241 tccactccgc cgggataaag gtcgccgtag taacctccaa gcgccgtgtc tccgtggaga
      301 tggcccttca gaattatccg ggcctccgta acgtcgtcga ccgcttcgtc acgatggagg
      361 acacaacgga gcacaaaccc gatcccgagc ctctcctgag agggctggaa ctcctcggca
      421 atgtcccgaa agaagaggcc gtttacgtgg gcgactcacc gttcgacgtg gaagcggcga
      481 aggccgcggg cctgaggagc gtcgccgtca gctggggcgc cttctccgag gacaccctgc
      541 gcgccgcagg acccgaccac ctcgtccctg acatcgacgc ggtggtggac gtcttgctga
      601 cgttgtagat acttctggct ggcaccgcta cagccggcca acggatttcg ccagcgcctc
      661 cagctcttcc tcgctgttga acaggtgtgt gctcgccctg acgcaggagt tcgggcccgg
      721 gatgtaacgc agtatgaacc tctgctgcga cagccaatct gccgcgtctt ttgacgttac
      781 gccttcgata tcgaagctta cgaggccgct ctgggccggc ttcggagagc ggagggtgac
      841 gcgtggcagc gtcgagagga ggtccatcag gaggtcggcc cggtgcctga tcccttcaaa
      901 gccagcctct ccgcgctcgt gcgcggcttc cgctgccgcc gcgaggccgc ccgcgagggt
      961 agggctcatg gtcgacgcct cgaagcgacg ggcgtcggac ctcagctcgt agtctccctc
     1021 tgcgtcgaaa tccgttggcg tatgcagcga catgaagccg acgttcgggc tgtggacggg
     1081 tagtcctggc ctgacgtaga aggcgcccat cccctcgggg ccgagtaccc acttgtgtcc
     1141 ggtgaacgcg tacatgtccg cgcctgtgtc cggcgcgtca acctgaatgt tccctaccga
     1201 ctgggcgccg tcgatcagtg tcaaaacgcc tctctcgcgg gcgagaccac agatctcccc
     1261 gagcggcagt atctccccgt tcgtccagtc aacgtgcgag agggcgatga gacgagtctt
     1321 atgcgtaagg gcggcctgga tccgctccgg cgttacggtg ggagagacca gatttacctt
     1381 caccccgaag cgcttcctca ggttgtggag cggcaccaga cagccaggat gctccgtagt
     1441 gaccgagacg acctcgtctc cctctcgcca ttcgatagat tcgacgccga ggttcatgcc
     1501 gtgggtggtg ttcagggtca gggccacgtc gtcgggtcgg accccgacga ggctggctgc
     1561 ggcctcgcgg gctcgcgagg aatacgccgc ctggcgggcg atgaggccga cgccttcaag
     1621 ataggcgggc cctgaacaca ggtcgtcggc gtcgcgcatg gcctcgagga catagtgggg
     1681 tggaggtccg ctgctgcccg agttcaggta tgcaaaatcg ttgtcggcga gcgccgggaa
     1741 catctcgcgg gggaggctct tgtcggtgat gacgcgtctc cttccgggat cagaatggcc
     1801 gttctgtata atagcccctt catgcagaac ggtaacctgg tcaggatcgg ccgccggcga
     1861 aggcggattc gcaacaagag caagctatgg cagaggctca ggactgcttt cctgctgctc
     1921 tgcctggtgg cgatcctcgg tacggtcggt ttcgtcgcca gcgccgtgag cacctaccag
     1981 actctcgccg ccaacatgcc cgacctcgac aactaccact ccaccgagct ggcccagaca
     2041 tctctgatct acgatacgaa cggcaacgtc gtcgaccagc tctacggcgt ccagaaccgc
     2101 ttcgttgtgc ccctcgatca ggtcgacccc acgctccaga aggcggtaat ctccatcgag
     2161 gaccaccgtt tttaccagca tagagggttg gacttcgagg cgataggccg cgctgccgtc
     2221 gagaacgtgc agagcctctc catccaggag ggcggctcca ccctgaccca gcagctcatc
     2281 aagaacacat acatagccca gcaggaacgc tatatcccta gcttcgagcg gaagttcgtc
     2341 gaagcctcgc tcgcctggca g
//
LOCUS       DATAIR010000275         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_192591, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000275 DATAIR010000000
VERSION     DATAIR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192591"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..495)
                     /locus_tag="VHM69_06715"
     CDS             complement(100..495)
                     /locus_tag="VHM69_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740121.1"
                     /translation="MAEVEHGSLTLTVDQQVLESAPTLCDLDAAPGETLYLRSTTMRA
                     LGGELDRILHFIEETIIPVTELQEGFCGGGLISDRRARKIVTLSWWNSPEKMQASERC
                     AHLPEAITSLLLYLAEFPKIENYQFDAII"
     gene            478..633
                     /locus_tag="VHM69_06720"
     CDS             478..633
                     /locus_tag="VHM69_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740122.1"
                     /translation="MFYFGHDFYRNEAHKHAHSDIHEPKGPVVVYVDVLHVTEEAALG
                     VEDAPLA"
     gene            complement(1105..1563)
                     /locus_tag="VHM69_06725"
     CDS             complement(1105..1563)
                     /locus_tag="VHM69_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740123.1"
                     /translation="MAETEFLYSDLLPTGVDDTPYRLDLVAQGAQDPAVRLRVWLLVA
                     GFGGIEDAEEALLEPQSMLESSGTWLETTKWVHLVEGGEDAREHPPLRGIHDGLAHEP
                     GRDARRHIRPLQDREEDVPPLLRRVQVRVVWGVPGVAGSQMPAQVLPAHQ"
     gene            1649..2239
                     /locus_tag="VHM69_06730"
     CDS             1649..2239
                     /locus_tag="VHM69_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2740124.1"
                     /translation="MTSNTRIPPAEITGIKGALMKRMATRMMGQVPTPLGVYWHNPKV
                     LKASFAIGSKVQKWDTCDENLKSFAHMTVASLVGCTWCLDFNYFQAHNENLDLEKARE
                     VPRWRESDVFTPLERDVMAYAEAMSQTPPTVTDELAARLLEALGAAALVELTAVISFA
                     NLTTRGNVALGIESDGFAEACDLKPLASPSSVASQA"
     gene            2236..>2401
                     /locus_tag="VHM69_06735"
     CDS             2236..>2401
                     /locus_tag="VHM69_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX2740125.1"
                     /translation="MSDDPFVAHRSLLFTVAYEMLGSAADAEDVVQETWLRWAAVDRT
                     EVRDPRAYLVR"
BASE COUNT          439 a          735 c          804 g          423 t
ORIGIN      
        1 ccatggtagc gcgggagcga ggcgggcgct acttcattga agcgcgtggg aatcgggctc
       61 tgcgaacttc gccttgtcga aagtctgcga agtctgcact tagattatgg cgtcgaactg
      121 gtagttctct attttgggga actccgcaag gtagagtagg aggctggtga tcgcctccgg
      181 gaggtgcgcg cagcgctcgc tggcttgcat cttttccggg ctgttccacc aagagagggt
      241 gacgatcttg cgcgctctcc ggtcgctgat caggcctccg ccgcagaacc cttcttgcag
      301 ctcggtgacg ggaatgatgg tttcctctat gaagtgtagg attcggtcca actcgcctcc
      361 gagggcgcgc atggtggtgc tccgtaagta cagggtttca cctggcgcgg cgtccaggtc
      421 gcacaacgtt ggtgcgctct ccaggacctg ctgatctacc gtcagggtga gagagccgtg
      481 ttctacttcg gccatgactt ctaccggaac gaagcccaca aacacgccca cagcgacatc
      541 cacgaaccga aaggtcctgt cgtcgtctat gtagacgttc tccacgtgac cgaggaggcg
      601 gccctcgggg tcgaagacgc ccctctggcg taggtcgcgt tgggggacgc gggtgctggt
      661 cgtaggccag ccgggttagg agcgctccta accgcgctat tacctacagc gtggtcagga
      721 gccatcaggc cgactggtgc gaccggttcg ccgctcgaag tggagcggac gggcgggacc
      781 catcgcccta ttccatatct gcgcccgcgt attctagctc cgcaccaacg gtcctcacaa
      841 cgctgatggt ctattcaccc gagtgcggga gggtggcgcg agagcgtggc gggcgctagg
      901 ctagagcggt ttgctgaaag agtgaacctg aggtgagtaa aggtttcagg ctacaggcat
      961 cagcaaaaga cctgaagcct gaagccttct ccgaaggagt agctcatcct tttcgagaac
     1021 cgccaaagga aagtcaaacc caggatgtcg gggggtggta tcgcgggcgg gagtgtctta
     1081 cagcctgctc gggcggcacc gggactactg gtgagcaggg aggacctgcg ctggcatctg
     1141 cgaaccggct acccctggca cgccccagac cacccggacc tggacacgcc gcagaagtgg
     1201 tgggacgtcc tcttcccggt cctggagcgg gcgtatgtgg cggcgggcgt cgcgcccggg
     1261 ctcgtgcgcg aggccatcgt ggatcccgcg cagtggcggg tgttcccggg cgtcttcccc
     1321 gccctcgacg agatggaccc acttggtggt ctccaaccac gtccccgagc tctccagcat
     1381 agactggggc tcgaggagcg cttcctccgc gtcttcaatt ccgccgaaac cggctacgag
     1441 aagccacacc cggaggcgta ccgccgggtc ctgcgcacct tgggcgacga ggtcgaggcg
     1501 gtacggcgtg tcatccacac cggtcgggag gaggtcggag tacaggaact ctgtttcagc
     1561 cacggcgatc aagaaattcc gccgaaactg tcacaaccga ttcccggccg gtgtctccat
     1621 gttcagaacc accgacaaag gaggagacat gaccagcaac acccggatcc cgccggccga
     1681 gatcaccggc atcaaggggg cgctgatgaa gcgaatggcc acaaggatga tgggacaggt
     1741 gccgacgccg ctcggggtgt actggcataa ccccaaggtg ctgaaagcca gcttcgcgat
     1801 cggcagcaag gtccagaagt gggacacgtg tgacgagaac ctgaagtcgt ttgcccacat
     1861 gaccgtcgcg tcgctggtcg gttgcacctg gtgtctcgac ttcaactatt tccaggcgca
     1921 caacgagaac ctcgacctgg agaaggcgcg cgaggtgccg cgttggcgcg agtcggacgt
     1981 gttcacgccg ctggagcgcg acgtgatggc gtacgccgaa gcgatgagcc agacgccgcc
     2041 gaccgtcacc gacgagctgg cggcccggct gctggaggcg ctcggcgcgg cggcgctggt
     2101 cgagttgacg gcggtcatct cgtttgccaa cctcaccacg aggggcaacg tggcactcgg
     2161 catcgagtcg gacggcttcg cggaggcgtg cgacctgaag ccgttggcca gcccgagcag
     2221 cgtagcgtcg caggcatgag cgacgacccg ttcgtcgccc accgcagcct gctcttcacc
     2281 gtcgcctacg agatgctcgg ctcggccgcc gacgccgagg acgtggtaca ggagacctgg
     2341 ctgcggtggg ccgccgtcga ccgcaccgag gtgcgggacc cgcgtgccta cctggtccgg
     2401 a
//
LOCUS       DATAIR010000276         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_433173, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000276 DATAIR010000000
VERSION     DATAIR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433173"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VHM69_06740"
     CDS             <1..561
                     /locus_tag="VHM69_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740126.1"
                     /translation="WLAYGGVLVVGAVFASGVISIWSHNVSIAVVSVGLLGVPIFTGV
                     AIARYRLYDIDIVINRTLVYGPLTAILAVIYVGSVVGLQAVFRILSGQESTLAVVAST
                     LLIAALFTTLRHRIQSFIDRRFYRRKYDARKTLEAFSARLRDETDLEALNDELVGVVR
                     ETIQPAHVSLWLRPLADAARSSGSSR"
     gene            558..1832
                     /locus_tag="VHM69_06745"
     CDS             558..1832
                     /locus_tag="VHM69_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740127.1"
                     /translation="MSRRAATWFAWALAGLTGVMFISGVALYLLARSSQVAPTTGGVL
                     SELLFYVAFLAFPIVGALIASRRPHNPIGWICLAVGLLWMLGGLSEQYTAYGIAKPGS
                     VPYLATIVALIQWMWIIPVGLLGTYLLLLFPDGRLPSRRWRPLAWLSGAVITLLGVDI
                     VLAPGPLIDLEGVRNPFGLEGAPWLVDAGIVLLLLFVTCILASAVSLVSRYRRSGGEV
                     REQIKWIALAGAFVGLLAFLSILLGLLIVPEVMRGGGSLPFWLWFLIYVLNVSFAGVP
                     VAIGFAILRNRLYDIDIIINRTLVYGSLTLMLALVYFGSVTMTQALFQTFTSQEQLPQ
                     LVVVASTLVIAALFNPLRRRIQSFIDRRFYRSKYDAAKTLAAFNSRLREETDLDSLGE
                     EVLGVVKETMQPEHASLWLRPYKADAKEDVPG"
     gene            2132..2497
                     /locus_tag="VHM69_06750"
     CDS             2132..2497
                     /locus_tag="VHM69_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740128.1"
                     /translation="MREPVFNQTLQLGIVVRDLEAMVRRYEDDYGIGPWKFAQIDLGE
                     ANDYREYGRPVERSNRVAFATPDFDETVARAERKDGVMLSCEHSGIDIAYLDTQSDLG
                     VTIEIFSGTPGAEQEPDAT"
     gene            2752..3678
                     /locus_tag="VHM69_06755"
     CDS             2752..3678
                     /locus_tag="VHM69_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2740129.1"
                     /translation="MTNSDHAPEQPPIPGPEVGPMKPNERIIRANGIDLCVQTFGDMA
                     DPPILLVMGGASSMVWWEDGFCERLMVGSRFVIRYDHRDTGRSISYELGAAPYSLRDL
                     AEDAVGLLDALGLESAHLVGMSVGGWIGQLMALDHPDRVASLTLISTSPTAGPGDPDL
                     PEMSEELRAFFAEEAPEPDWSDRDAVIESIVEGERRFAGSRPFDEAAIREIAARAFDR
                     TTNLASSITNHAGIDTGDRWRERLGEVSAPTLVIHGTEDPMFPYGNAVALAKEIPGAR
                     LLGLERVGHEVPPRDLWDVVVPAILRHTASAP"
     gene            complement(3697..4389)
                     /locus_tag="VHM69_06760"
     CDS             complement(3697..4389)
                     /locus_tag="VHM69_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2740130.1"
                     /translation="MEERDQAYLFEQFRVERGCLGYIVASARSAAIIDPELEMVEPML
                     DYVFEHGLKPKYVIDTHTHADHFSGARELKSKTTAKIVMHEKAPASFVDIRVEDGDRL
                     QIGDLSIKFIHTPGHAKDLVSVMLPGRILTADALLIGSCGRTDLPNGNAVRQYHSLYY
                     TYRALPDELLVFPGHDYNGREHSTLEEEKESNPKMLFDTEEEFVEYMERENPEKLTPV
                     YHLADALKANMA"
     gene            complement(4557..>5359)
                     /locus_tag="VHM69_06765"
     CDS             complement(4557..>5359)
                     /locus_tag="VHM69_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2740131.1"
                     /translation="DVALAHATYCGEVTMVDRWVGHLLSKLEVLGLAENTVVVFTSDH
                     GFYFGEHDYFGKAEWVHDPEACVTEDSSVPDWLTESWLLTVERSPLYKELTNVPLMVR
                     GPGLEPGRTQAMTTAPDLAPTIMDLVGLGGLPTTMTGDSFGGVLDGSRKEHRPLVVSS
                     WPLYLAEGEIVTAIDSKARRISDYMPVTVTTPERSLILGGPDDEPELYDLTRDPGEQH
                     NVWRNSGGEGELLGEQALTFLEGVNTPEEYLAPRRESIDRWRVVRKSA"
BASE COUNT          935 a         1655 c         1666 g         1103 t
ORIGIN      
        1 tggctcgcct acgggggcgt gctcgtcgtt ggcgccgtct tcgcgagtgg cgtgatctcc
       61 atctggagcc ataacgtgag catcgcggtc gtcagcgtcg gcctgctcgg ggtgccgatc
      121 ttcacgggag tcgcgatcgc gaggtaccgc ctctacgaca tcgacatcgt cataaatcgc
      181 accctcgtct acggacctct caccgcaata ctggcggtga tctacgtggg gagcgtggtt
      241 ggtctgcagg ccgtcttccg aatcctcagt ggccaggagt ccaccctcgc cgtcgtggcc
      301 tccaccctct tgatcgctgc gctgttcacc accttgaggc atcgtatcca gtcgttcata
      361 gataggcgct tctaccgcag gaagtacgat gccaggaaga ccctggaagc tttctctgcc
      421 aggctcagag acgagacgga cctggaggcg ctaaacgacg aactggtggg agtggtcagg
      481 gagacgatac agcccgccca cgtttcgttg tggttgcggc ccctcgccga cgcggctcgg
      541 agtagtgggt caagtagatg agccgccgcg ctgccacttg gtttgcctgg gcgctcgccg
      601 gtctcactgg ggtcatgttc atatccggcg tcgcactgta tttgctcgcc cgttcatcgc
      661 aggtggctcc caccaccggc ggcgttctca gcgaactctt attctacgtt gcattcctag
      721 cattccccat agtgggtgcg ctgatcgctt ccaggcgccc gcacaacccc ataggctgga
      781 tttgcctggc agtcggcctt ctgtggatgc tcggtggatt gagcgagcag tacacagcgt
      841 acggcattgc caagcccggc tcggtcccat acctggcaac tatcgtcgcg ctcatccagt
      901 ggatgtggat aatccccgtt gggctccttg gcacctacct gctcctgctg ttccccgacg
      961 ggaggctccc ctcaagaagg tggcgtcctc tggcgtggct atctggggct gtgatcacct
     1021 tgctcggtgt cgatatcgtc ctcgcccctg gaccattgat cgaccttgag ggagtgcgta
     1081 accccttcgg gctagaggga gcaccttggt tggtcgacgc agggatagtc cttctcctgc
     1141 tgtttgttac gtgcatcctc gcttcagcgg taagcctggt ctcgcgttac aggcgctctg
     1201 ggggagaagt gcgagagcag ataaagtgga tagcgcttgc cggtgcgttc gttggccttc
     1261 tggcattcct gagcatccta ctcggcttgc tcatcgtacc tgaggtaatg aggggtggcg
     1321 gctccctacc attttggctt tggttcctga tctacgtgct gaacgtgagt ttcgcaggag
     1381 ttcctgtggc gataggcttc gctatcctca gaaaccgcct ctacgatata gacatcatta
     1441 tcaaccgcac gctcgtctac ggctcgctca cgctgatgct tgcgctggtg tacttcggga
     1501 gcgtgacgat gacccaggcc ctcttccaaa cgttcacgag ccaagagcag cttccccaac
     1561 tcgtcgtcgt ggcctctacc ctagtgatcg ccgcgctgtt caatcctttg agacgccgca
     1621 tccaatcctt catagaccgt cgcttctaca ggagcaagta cgacgcggcc aagacgctag
     1681 ctgccttcaa ctccaggttg cgcgaggaaa ccgacctcga ctccttgggc gaggaggtgc
     1741 tcggggtggt caaggagacg atgcagcccg agcacgcctc cctatggcta cgtccctaca
     1801 aggcggacgc gaaggaggac gtgcccggct gaggaggtga acgcctaact gagatgccat
     1861 cgtacctcag tggccggcgg ctgctacctc accatgttgt ttccaaatgc aacgcatccc
     1921 cggctgcggt tcagcgccag gttgggagag tagccggaga tcgaggtgac gctggattag
     1981 gtacttcgcg ctcctgcctt gctcacggaa gtgcgtacca gacgtttcct tgatcgtctg
     2041 acgtcccgtt tgaagcgttc tcagaagttt accggtggtc ggggttccga gttccagtga
     2101 gatcgcctca tcgacttagg ggaggggaac aatgcgagag cccgtcttca accaaacgct
     2161 gcaactcggg atcgtcgtgc gcgatctgga ggcgatggta cggaggtacg aagacgacta
     2221 cgggatcggt ccttggaagt tcgctcagat cgaccttggt gaggcgaacg actaccgcga
     2281 gtacggtcgg ccggtcgagc gctccaaccg cgtcgccttc gccaccccgg acttcgacga
     2341 gaccgttgcg cgggcggagc gcaaggacgg cgtgatgctc agctgtgagc acagcgggat
     2401 tgacatcgcc tacctcgaca cccagagcga ccttggggtg accatcgaga tcttcagcgg
     2461 tacgcctggc gccgaacaag agccggatgc gacgtagccg cgatcttcgt agcgcgcaac
     2521 gagacgaagc gggcacgcct gtccggtcat cagcttcaac ctcccgctct atgcacccaa
     2581 ctgctcagag ttggtgttct aggtagttag gatccaagat cttgcacagc cgaggtcgga
     2641 taggcccctg agagtccatc tgatggcctc ggttagatcg atagtggatc ttaagaatat
     2701 cgccgtatgc tccgagtaga cgttgccgca accattaagg agcgccccgt catgacgaac
     2761 agcgaccacg ccccagagca gccgcccata ccgggtcccg aggttgggcc tatgaaaccc
     2821 aacgagagga tcatccgggc caacggtatc gacctctgcg tccagacatt cggcgatatg
     2881 gccgaccccc caatcctcct cgtcatggga ggggcatcct cgatggtctg gtgggaggac
     2941 gggttctgcg agcgactgat ggtcggctcc cggttcgtca tccgctacga ccaccgcgac
     3001 accggcagat cgatcagcta cgaactgggg gctgcaccct attcgctgcg cgatctggca
     3061 gaggacgccg tcggcctact cgacgccctc ggcctggaga gcgcccatct ggtgggcatg
     3121 tcggtgggcg gttggatcgg ccagctcatg gcgctagacc atcccgatcg ggtcgcatcg
     3181 ctgaccctga tctcgacaag ccccaccgct gggccaggcg atccggacct acccgagatg
     3241 tccgaggaac tacgagcctt cttcgcagaa gaggcccccg agccagactg gtcggatcgg
     3301 gacgccgtga tcgaatctat agtcgaagga gagcgtcggt ttgcgggctc tcgacccttc
     3361 gacgaggcgg ccatacgcga gatcgcagcc cgcgccttcg atcgcacgac caacctcgcc
     3421 tccagcatca ccaaccatgc aggcatcgac acgggagatc gatggaggga gcgactcggg
     3481 gaggtgagcg ccccgacgct cgtcatacac ggcaccgagg atcccatgtt cccctacggc
     3541 aacgcggtcg cgctggcgaa ggagattccc ggcgcccggc tgctcgggct ggagcgggtg
     3601 ggtcacgagg tgccccctcg ggacctctgg gacgtcgtcg tgcccgcaat attgcggcac
     3661 acggcgtctg caccctgaga gcagacctga agcctatcaa gccatgtttg ctttgagggc
     3721 gtcggccaga tggtagacgg gcgtcagctt ctccgggttc tcgcgttcca tgtactcgac
     3781 gaactcttcc tccgtgtcga agagcatctt ggggttgctc tccttctctt cttccagggt
     3841 cgagtgctcg cggccgttgt agtcgtggcc agggaagacg agcagttcgt cggggagagc
     3901 gcggtaggtg taatagaggc tgtggtactg gcggacagcg ttgccgttcg ggaggtcggt
     3961 gcggccgcag gagcctatga ggagagcgtc ggcggtgagg atgcggccag gcagcatgac
     4021 ggagacgagg tccttcgcgt ggcccggagt gtggatgaac ttgatcgaaa ggtctccgat
     4081 ctggagtcgg tcgccgtctt ccacccttat gtctacaaag cttgcaggag ccttctcgtg
     4141 catgactatc ttcgccgtgg tcttcgactt cagctcccgc gctcccgaga agtggtcggc
     4201 gtgggtgtgg gtgtcgatca cgtattttgg cttgagaccg tgctcgaaga cgtagtcgag
     4261 catgggctcg accatctcga gctcggggtc gatgatcgcc gccgaacggg cgctggcgac
     4321 gatatagccg aggcagccgc gctcgactcg gaactgctcg aacaggtagg cctgatctct
     4381 ctcctccaag ggcgctcctt catcccgata ttagcctcat caccttggag gtatcctagt
     4441 tcattcctct ccacttcgtc gggtttgatc tcgcgtccca cgaaaggggc cggcgcgccc
     4501 gcggccggcc cctttccacg tactcttaga tgcatttccg cccttgcggc agaagatcaa
     4561 gcgctctttc ttaccaccct ccaacggtcg atcgactcgc ggcggggggc caggtactcc
     4621 tccggtgtgt tgacgccttc gaggaaagtg agggcctgct cgccgagcaa ctcgccctcg
     4681 ccgcctgaat tcctccagac gttgtgctgc tcgcccgggt cgcgcgtgag gtcgtatagc
     4741 tcgggctcgt cgtcggggcc gcccaggatc agggaccgct ccggcgtggt tacggtgacc
     4801 ggcatgtagt cggagatcct gcgcgccttg gagtcgatgg ccgtgacgat ctcaccctct
     4861 gccaaataga gcggccagga gctcacgacg agcggcctgt gctccttcct gctcccgtcg
     4921 agtacgcccc cgaaggagtc gcccgtcatg gtggtcggca ggcccccgag cccaacgagg
     4981 tccatgatcg tcggcgccag gtctggggcg gtagtcatcg cctgggtgcg ccctggctcc
     5041 agcccaggtc cgcggaccat cagcggtacg ttcgtgagct ccttgtagag cggagatcgc
     5101 tccacggtca gtagccacga ctctgtgagc cagtctggga cagacgagtc ctccgtcaca
     5161 caggcttcag ggtcgtgtac ccactcggct ttgccgaagt agtcgtgctc cccgaagtag
     5221 aagccgtggt cggaggtgaa cacgacgacc gtgttctcgg cgagaccgag cacctcgagc
     5281 ttcgagagca ggtgcccgac ccagcggtcg accatcgtca cctcgccgca gtacgtcgcg
     5341 tgtgcgagcg cgacgtcgt
//
LOCUS       DATAIR010000277         7769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110311, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000277 DATAIR010000000
VERSION     DATAIR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110311"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VHM69_06770"
     CDS             <1..1496
                     /locus_tag="VHM69_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740132.1"
                     /translation="RDTGIGMTAEQEGRLFQSFTQADTSTTRRYGGTGLGLAISKQVV
                     EMMGGEIGVETEPQVGSTFWFTLPLYKQTVGTYKRADPVPRANLGELRVLVVDDNETN
                     RKILHEQVVPWGMESVAVENGWEALQALREAAKSDEPYDLAILDREMPNMDGMELATR
                     IKADPSIASTRLIMLSSAAGRGESEEARRIGIEACLSKPVRQSRLYDTIAALMGSPEA
                     QATTREPEQEAPLLRGEASEAGFRERRVPVLVVEDNVVNQRVAVKMLQKLGYRADVAA
                     NGLEALEALSRIPYSAILMDVQMPEMDGYAATREIRGRQSGKDRTPIIAMTANAMQGD
                     KEKALKAGMDDYVSKPVKLEALAAVMQRWIPREYEAVAEPAAPEADEESADLAESLDL
                     RGLQEQWEPDILDELIWLFLDSAPSQLVALREAVEAGDASAVERIAHTLKGSSASMSA
                     ARMAALCTRVEQAGLSEDPGRAWPLIPLLDEEFARVREALERELSRR"
     gene            1628..2875
                     /gene="hisZ"
                     /locus_tag="VHM69_06775"
     CDS             1628..2875
                     /gene="hisZ"
                     /locus_tag="VHM69_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX2740133.1"
                     /translation="MNRETPRKFSTTPGTRDVLPPESTRLLDVQATIRDRFRLFGFRE
                     VITPALEYSEVIEEHNLRDASYKLFDPDNQMLLLRPEMTTPIARLISQRLAGSPPPHK
                     LSYVMPAYRRASVGRGQSAELYQAGVEVVGTPSPREDASTIALLVDVLQGVGLSATSD
                     FAVVLGQSAFYSGYLRHAAPEAATAVTAALAAKDLVAVDAIAGDLTAEAAAGVRGIPR
                     LVGPATDGSIVEEAGRYAAGEEAAAALENLKEILRHLEAHGALGAVILDLGLIGRHDY
                     YTGAVYEAYAAGLGFTIANGGRYDNLLRRFGKQMPATGFAIYLERLLSVLPEEEPPPL
                     LVLVGGDVEGIEAAAELRRLGVPVLHLSEDLPPGDAAEYARSVEAAWISYPTTGGVKL
                     ATADPPGELVFMDPESAARTVLS"
     gene            2872..3519
                     /gene="hisG"
                     /locus_tag="VHM69_06780"
     CDS             2872..3519
                     /gene="hisG"
                     /locus_tag="VHM69_06780"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2740134.1"
                     /translation="MSRLRVAVPKGAIFEDALLALEAAGLPADVVRENGRRLVYRTGD
                     AEFIVSRPSDVPVFVEYGAADVGIVGKDVLEEQRPNVVELKDLGTGRCRMILAAPDSG
                     AEHVRRAIRHAEVVRVATKFPDTARRYFERMGRQAEVIELHGSIELAPLVGLAECIVD
                     LTATGTTLRENHLVELDEISTSTARLISNRGAYRLRHSEVAALADAMPKGGTTVD"
     gene            3521..4756
                     /gene="hisD"
                     /locus_tag="VHM69_06785"
     CDS             3521..4756
                     /gene="hisD"
                     /locus_tag="VHM69_06785"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2740135.1"
                     /translation="MLDARDLSAEEVAGHLRRPGGFLSEDVRDIARAIVTDVRKGGDH
                     ALLKHTERLDGVRPDPIRVPEEEIEAAMSALEPEVEDSFRLAIENVRAFHEQEMDSPW
                     HIERDGATVGQRVRPLRRVGLYVPGGHGAYPSTLIMAAVPAQVAGVGEICICVPPGPD
                     GRVSTPVLAVAGLLGLEEVYRVGGAQAIGAMAFGTESIARVVKIVGPGNDYVTAAKLE
                     VFGTVGIDAPQGPSEIVVIADASAFPERVASDLMAQAEHLSGASAVLLSPSAGLLREV
                     MPLLTGEPAKLVTLVHVEDVAQAVEISNHYAPEHAHVISEDPESILQDLDAAGMVAVG
                     DFSPVALGDYAAGPSHALPTGGAAAWASPLGTHDFLARTNYIHFTRDALQVLGPHVER
                     LARLEGFENHAASVSTRMG"
     gene            4795..5379
                     /gene="hisB"
                     /locus_tag="VHM69_06790"
     CDS             4795..5379
                     /gene="hisB"
                     /locus_tag="VHM69_06790"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX2740136.1"
                     /translation="MARKGSAERKTEETFVRVNLDLDGEGRAEVATGVGFLDHLLHLL
                     AHHSGIDLTVEAKGDTWVDDHHTVEDTGLVLGRAFDEALGDRGGIVRFADASAPLIEA
                     LSTVVVDLGARSHLTCNLGPMPEKIGTFDVELFPEFLRAFTQYGRFTLHFNCQYGENA
                     HHKVESGVKALAVALREGIAPRASGSASTKGVLD"
     gene            5381..6019
                     /locus_tag="VHM69_06795"
     CDS             5381..6019
                     /locus_tag="VHM69_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2740137.1"
                     /translation="MSARPEGVEAVVFDLGGVLIDWDPRHLYRKIFVSEEEVDDFLST
                     VATPEWHSEQDRGRTLEEATALLLSQHPGYGPEIKAFYGRWDEMFGGPIEDSVCVLQD
                     VRERGHPLYALTNWPAEIFPRARKRWDFLSWFDDIVVSGEERATKLDREIYDVLVERT
                     GLDPATTVFIDDREPNIRAAEDLGFIALKFEDAAKLRKELVQLGVLNGHSGA"
     gene            6000..6635
                     /gene="hisH"
                     /locus_tag="VHM69_06800"
     CDS             6000..6635
                     /gene="hisH"
                     /locus_tag="VHM69_06800"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX2740138.1"
                     /translation="MATPVPDAMNVAVVDYDAGNTLSVTRALEKVGAKVDLTPDPERV
                     LAADAVVLPGVGAFGDCMRKLRDRGMDEACRETFRSGKPFLGVCVALQVVFEASEEST
                     GVEGLGLMPGRVVRFERDGLKVPHIGWNELSVVRDHPILEGLDGEAFYFVHSYFPEPE
                     EKADIIGETDYGTRFCSAAGRENLVAVQFHPEKSSRAGLKLYENFIAWARA"
     gene            6658..7242
                     /locus_tag="VHM69_06805"
     CDS             6658..7242
                     /locus_tag="VHM69_06805"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2740139.1"
                     /translation="MSNEAFLRGPRVRLRTLERADLPRYQELFMEPEINMLYGGLGLP
                     ENLAKLQRCFDAGEYDTTDRFLTLVIEADGTIIGAMVLKNTENLPSRSATFGIIIGDP
                     EYLDRGYGTEASTLLLDYAFGVLGYHKINLDLFEYNARALAMYEKLGFVVEGRRRENH
                     WSRGRFWDEILMGVTAEEWWGKYGPPATPGPARR"
     gene            7239..>7769
                     /locus_tag="VHM69_06810"
     CDS             7239..>7769
                     /locus_tag="VHM69_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2740140.1"
                     /translation="MTGRSFTVFPAIDLRGGRCVRLKQGDFGRSKEYDADPVERAREW
                     ERQGARAIHVVDLDGAEEGRPVQLELIRRIAGAVDVPLQVGGGIRAIEDLRAVRAAGA
                     VRVVMGTAAVEDRELRLMVVEEAGDALVVAIDARGGIVATHGWLRSSGLPVLDLAKEL
                     AADGVAQVLYTDVARDG"
BASE COUNT         1450 a         2407 c         2637 g         1275 t
ORIGIN      
        1 tcagggatac aggcataggg atgaccgcag aacaagaggg acgcctcttc cagtctttca
       61 cccaggccga cacctcaacc acccgccgct acgggggcac ggggctcggg cttgccatta
      121 gcaaacaagt ggtcgagatg atgggtggag agataggtgt agagaccgag ccccaggtcg
      181 gaagcacatt ctggttcacc ttgccgctgt acaagcagac cgtcggcact tacaagcgag
      241 cagatcccgt gccacgcgcg aatctgggcg agctgcgcgt gcttgtcgtt gacgacaacg
      301 agaccaaccg caagatcttg cacgagcagg tcgtaccgtg ggggatggaa agcgttgccg
      361 tcgagaacgg gtgggaagcg ctccaggcgc tgagggaggc cgcaaagagc gacgaacctt
      421 acgacctggc gatccttgac agggagatgc cgaatatgga cggcatggag ctggcaacca
      481 ggatcaaagc cgacccctcg atagcctcca ccaggctcat aatgctcagt tcggccgccg
      541 ggcgcggcga gagcgaggag gcaaggcgga taggcatcga agcttgcctt tccaagccgg
      601 tgaggcaatc caggctctac gacacgatag cagccttgat gggttcgcct gaagcgcagg
      661 ctaccaccag agaacccgag caggaagctc cccttctgcg tggcgaagcg tcggaagctg
      721 gttttcgtga acgacgtgtt cccgtgcttg ttgtcgagga taacgtggtc aaccagaggg
      781 tggccgtgaa gatgctccag aaactcggct accgcgcgga tgtcgcggcg aacggcctgg
      841 aagccctcga agctctatcc cgcataccct actcggctat cctcatggac gtgcagatgc
      901 cagagatgga cgggtatgcg gcgaccaggg agataagagg aaggcaatcg ggcaaggatc
      961 gcacacctat catcgccatg acggccaacg cgatgcaggg agacaaggag aaggccctaa
     1021 aggcgggtat ggacgactac gtctccaagc cggtcaagct agaggctctg gcggcggtga
     1081 tgcagaggtg gatcccgcga gaatacgagg cagtggctga gcctgcagct cccgaagcgg
     1141 acgaagagtc cgctgatctc gctgagagtc tggacctccg cggtttgcaa gagcaatggg
     1201 agccggacat cctggacgag ttgatctggc tattcctgga tagcgcgcct tcgcagctcg
     1261 ttgccctcag ggaagcggtg gaggccggtg atgccagtgc agtcgagcgg atcgcgcaca
     1321 cactcaaggg cagctcggcc agcatgagcg ccgcaaggat ggcggcgctc tgcacgcgag
     1381 tcgagcaggc cgggctctcg gaggatcccg gccgtgcctg gcccctgatc ccccttctcg
     1441 acgaggagtt cgcccgcgtc agggaggcgc tggagcggga gctttcgagg cgctaggata
     1501 ctcgcgtcag tacggcatga tggcgcacta tcctgcttcg cgcgtcccga cccgtctcat
     1561 ccccaaagaa gtcctggggg acgggctcct ttaacgtggg gggacatcgg ttaagatagc
     1621 gtctcctatg aatcgtgaga cgccgcggaa attttctacc acccctggca cgcgcgacgt
     1681 gctgccgccc gagtccacgc gcctgctcga cgttcaggcc acgatcaggg atcggttcag
     1741 gcttttcggg ttcagggagg tcatcacacc tgcgctcgag tactccgaag tcatcgagga
     1801 gcacaacctg agggacgcct cgtacaaact cttcgacccc gacaaccaga tgctcctcct
     1861 gcgtcccgag atgacgaccc cgatagcgcg cctgatctcc cagcgcctgg ccggcagccc
     1921 gccgccgcac aagctctcct acgtcatgcc tgcctaccgc cgcgcgagcg tcggcagggg
     1981 acagagcgcg gagctgtacc aggccggcgt tgaggtggtc ggtaccccga gtccgaggga
     2041 agacgcctcc accatcgcgc tcctcgttga cgtactgcag ggcgtcggcc tcagcgctac
     2101 ctcggacttc gcggtcgtgc tcggccagtc ggcgttctac tccggctacc ttaggcacgc
     2161 cgccccggag gcggcaacag ccgtgacggc tgccctggcc gcgaaggacc tcgtggccgt
     2221 cgacgcgata gccggggacc tgaccgctga ggccgccgcc ggtgtccgcg gcataccgcg
     2281 tctcgtcggg cccgcgacgg acggttccat tgtcgaggag gcaggacgtt acgccgcggg
     2341 ggaggaggcg gctgcggcgc tcgagaacct gaaggagata ctgcgccacc tggaggcgca
     2401 cggggcgctc ggggccgtca tccttgacct cggtttgatc gggcgccacg actactacac
     2461 gggcgctgtc tacgaagcct acgccgccgg gctcgggttc accatcgcca acggcgggcg
     2521 ctacgacaac ctgctcaggc gtttcggaaa gcagatgcca gcgaccggct tcgccatata
     2581 tctggagagg ctcctctccg tgctccccga ggaagaaccg ccacccctgc tcgtcctcgt
     2641 cggcggagac gtggagggca tcgaagcggc ggctgaacta cgcaggctcg gcgtgcccgt
     2701 ccttcacctc tccgaagacc tgccgccggg ggacgcggcc gagtacgcca ggagcgtcga
     2761 ggcggcctgg atctcctacc ccaccacagg aggcgtcaaa ctggctacag cagacccacc
     2821 aggagagctc gtgttcatgg atcccgagtc cgcggccagg acggtgctct cgtgagcagg
     2881 ctgcgcgtcg ccgtgccgaa gggcgccatc ttcgaggacg cgctcctggc gctcgaagcg
     2941 gcgggactcc ctgccgacgt cgtacgtgag aacggaaggc ggctcgtcta caggacagga
     3001 gatgcagagt tcatagtcag caggccctcc gacgtgcccg tcttcgtcga gtacggggcg
     3061 gcggacgtcg gtatcgtcgg caaggacgtg ctagaggagc agagacccaa cgtggtcgag
     3121 ctcaaggacc tcggcacggg gaggtgccgt atgatcctcg ccgccccaga tagcggggcc
     3181 gaacacgtcc ggagggccat ccgccacgcc gaagtcgtca gggttgcgac caagttcccc
     3241 gacacagcca ggcgctactt cgagcgcatg ggccgccagg ccgaggtcat cgagttgcac
     3301 ggctccatag agctcgcccc cctcgttggc ctcgcggagt gcatcgtcga cctcaccgcc
     3361 acaggcacga cgctgcgcga gaatcacctg gtcgagcttg atgagatctc cacctccacg
     3421 gcgcgcctga tctccaaccg cggtgcctac cgcctgcgac acagcgaggt cgcagccctc
     3481 gcggacgcca tgccgaaggg ggggacgacc gttgactgaa gtgctggatg ccagggacct
     3541 ctccgcggag gaggtcgccg gccatctaag aaggccaggc gggttcctct cagaagacgt
     3601 ccgcgacatc gcgcgcgcca tcgtcaccga cgtgcggaag gggggagacc atgccctctt
     3661 gaaacacacg gagcgcctcg acggggtgag gccggatcct attcgggtcc ctgaggagga
     3721 aatcgaggct gcgatgtcag cactcgaacc tgaagtagaa gacagcttca ggttagccat
     3781 cgagaacgtc cgcgcattcc acgagcaaga gatggacagc ccgtggcaca tcgagcgcga
     3841 cggcgccacg gtggggcaga gggttcggcc tttgaggcgg gtcggcctgt acgttccagg
     3901 tgggcacggc gcctacccga gcaccctgat catggctgct gtgcctgcgc aggtggccgg
     3961 ggtcggggag atctgcatct gcgtcccgcc gggacccgac ggacgcgtga gcacccccgt
     4021 actcgcagtc gcgggcctac tgggattgga ggaagtgtat cgggtcggtg gagcccaggc
     4081 gatcggggct atggccttcg ggacggagag catcgcgcgg gtggtcaaga tcgtgggtcc
     4141 tggcaacgac tacgtgacgg ctgcgaagct cgaagtcttc ggaaccgtcg gcatagacgc
     4201 ccctcagggg ccgagcgaga tcgtcgtgat cgccgacgcg agcgcctttc ccgagcgcgt
     4261 cgccagcgac ctcatggccc aggcggagca cctttccgga gcctcagccg tgctgctctc
     4321 accgagcgcg gggttgctca gggaggtcat gccgctcctc acgggcgagc cggcgaagct
     4381 cgtgacgctc gtccatgtgg aagacgtggc gcaggcggtc gagatctcca accactacgc
     4441 cccggagcat gcacacgtga tctcggaaga tcccgaatcc atcctccagg acctcgacgc
     4501 cgcaggcatg gtcgccgtgg gagatttcag ccccgtcgcc ctcggcgact acgccgccgg
     4561 tccttcgcac gccctgccga cgggcggagc ggcggcgtgg gcctcgcccc tcgggaccca
     4621 cgactttctc gcccgcacga actacatcca cttcacgcgc gacgccctgc aagtcctcgg
     4681 accgcacgta gagcgcctgg cacgtctcga aggcttcgag aaccacgccg cgagcgtctc
     4741 gacgcggatg ggatagcggt agaatgcttg ggttctaaga gatccgggag gaaaatggcg
     4801 cgaaagggca gtgcggaacg gaagacggaa gagacgtttg tcagggtgaa cctcgacctc
     4861 gacggcgaag gacgggccga ggtcgccacg ggcgtagggt ttctggacca tctgttgcac
     4921 ctgctcgccc accactcggg catagacctc acggtggagg ctaagggtga cacgtgggtg
     4981 gacgaccatc acacggtcga ggacacgggg ctcgttctcg gacgcgcctt cgacgaagcc
     5041 ctgggcgacc gtggcggcat cgtgcgcttc gccgacgcga gcgcgccgct catagaggcg
     5101 ctctcgacgg ttgtcgtcga cctcggggcg cggagccacc tcacctgcaa tctcggcccg
     5161 atgcccgaga agatcgggac cttcgacgtc gagctatttc cggagttcct gcgggctttc
     5221 acccagtacg ggcgcttcac cctccacttc aactgccaat acggtgagaa cgcccaccac
     5281 aaggtcgaat ccggtgtgaa ggcgctcgcg gtcgccctgc gagagggcat cgccccgcgc
     5341 gcatccggca gcgcttcaac gaagggagta ctcgattagc gtgtccgctc gcccagaagg
     5401 tgtcgaggcc gtagtgttcg acctgggtgg cgtcttgata gattgggatc ccaggcacct
     5461 ttaccgcaag atcttcgtca gcgaggaaga ggtagatgac ttcctatcca cggtcgcgac
     5521 gcccgaatgg cacagcgagc aggacagggg ccgtacgctg gaagaggcga ccgcgctgct
     5581 gctctcccaa caccccgggt acgggccgga gatcaaagcg ttctacgggc gctgggacga
     5641 gatgtttggc ggacccatag aagactccgt gtgcgtcctg caggacgtac gcgagcgagg
     5701 gcatccgctg tatgccctca cgaactggcc cgccgagatt ttcccgcgtg ccaggaagcg
     5761 ttgggatttc ctgagctggt tcgacgacat cgtcgtctcg ggtgaggagc gcgcgacgaa
     5821 actggacaga gagatctacg acgtactcgt cgaacgtacc ggcctggacc cagcaacgac
     5881 cgtgtttata gacgatagag agcccaacat ccgcgccgca gaagatctgg ggttcatagc
     5941 actaaagttc gaggacgccg caaagctccg gaaggagttg gtgcaactgg gagtcctaaa
     6001 tggccactcc ggtgcctgat gccatgaacg ttgcggtcgt ggattacgat gcgggtaaca
     6061 cgctctccgt gacgcgggcg ctcgagaagg tcggtgcgaa ggtggacctg acgccagatc
     6121 cggagcgggt cctcgcggct gacgctgtgg tgctgcccgg cgttggtgcc ttcggggact
     6181 gcatgcggaa gctgcgggac cgagggatgg acgaggcttg cagggagacg ttcaggtcgg
     6241 gcaagccttt cctcggcgtc tgcgtcgcgt tgcaggtggt cttcgaggcc tccgaggagt
     6301 cgaccggggt ggagggactc gggcttatgc ctgggagggt agtgcgcttc gagagggatg
     6361 ggctcaaggt gccgcatatc gggtggaacg agttgtctgt ggtgcgggat cacccgatcc
     6421 tcgaaggcct cgacggtgag gctttctact tcgtccactc ctacttcccc gagccggagg
     6481 agaaagcgga catcatcggc gagacggatt acggcacgcg cttctgttct gccgcgggca
     6541 gggagaacct cgtcgccgtc cagttccatc ccgagaagag cagcagggcc ggcctcaagc
     6601 tgtacgagaa cttcatcgcg tgggcgaggg cttaggtaga tggtcgaagg agaacgggtg
     6661 tcgaacgagg ctttcctgcg tggtccccga gtcaggctac gcacgttgga acgcgccgat
     6721 cttccccgct accaggagct gttcatggag ccggagatca acatgctcta cggcggactc
     6781 ggcctgccag agaacctcgc caaacttcag cgctgcttcg acgcggggga gtacgacacg
     6841 acggaccggt tcctcaccct tgtgatcgag gccgatggca ccattatcgg ggcgatggtg
     6901 ctgaagaaca cggagaacct gccgagtcgc tcggcgacgt tcgggatcat tatcggggac
     6961 cctgagtacc tggacagggg ctacggcacc gaggcgagca ccctccttct cgactacgct
     7021 ttcggtgtcc tcgggtacca caagatcaac ctggatctct tcgagtacaa cgcccgcgcg
     7081 ctcgcgatgt acgagaaact cggcttcgtg gtagaaggac gccgccgcga gaaccactgg
     7141 tctcgcggtc gcttctggga cgaaatcctc atgggtgtca cagcggaaga gtggtggggg
     7201 aagtacggac ctccggcgac accgggaccc gcgcgtcgat gaccgggaga tccttcaccg
     7261 tctttcccgc tatcgacctg cgcggcggga gatgtgttcg tctcaagcaa ggggacttcg
     7321 ggcgtagcaa ggagtacgat gccgaccccg tcgagcgtgc ccgcgagtgg gagaggcagg
     7381 gggccagggc gatccacgtc gtcgacctcg acggggccga ggagggaaga cccgtccagc
     7441 tcgagctcat ccgccgtatc gccggcgccg ttgacgttcc gctccaggtg gggggcggaa
     7501 tccgcgccat cgaagacctg cgtgccgtgc gtgcggcggg cgcggtgcgc gtcgtgatgg
     7561 gtaccgccgc ggtcgaggac agggagttgc gtctcatggt ggtcgaggag gcgggcgatg
     7621 ctctggtcgt ggccatcgac gcccgcggag ggatcgtcgc gacgcacggc tggctgaggt
     7681 cgagcgggct cccggtcctc gacctcgcga aggagttggc tgctgacggc gtcgcccagg
     7741 tcctctacac cgacgtggcg cgagacggc
//
LOCUS       DATAIR010000278         8426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_239096, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000278 DATAIR010000000
VERSION     DATAIR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239096"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VHM69_06815"
     CDS             <1..675
                     /locus_tag="VHM69_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2740141.1"
                     /translation="NGIPVLAYNANGKGPGTNPALAYVGQDLFQSGVEMGNRIVELVD
                     EGLVALFIATPGQLNIQPRIDGAKQAIEDSGANIEIQQVTTGAELPEERSRIEAWYNG
                     HQDVAGMFAVDAGSTQGVAQVMEQFGLHDKGVKAGGYDLLPEILKLMKAGHIDFTIDQ
                     QPYLQGFFPVMQLYLYNLSGGVTGPAETNTGLIFITPDTVDRYLETTSRFEGDSEAQK
                     IVKAPS"
     gene            703..1719
                     /locus_tag="VHM69_06820"
     CDS             703..1719
                     /locus_tag="VHM69_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740142.1"
                     /translation="MSETTAQETASTGTQERGFGGRLMDSLVRVREVSILIVLVALVL
                     YFQAANSAFLSEQNILSLSQYAVPYVVIASSLCLLLICGEIDLSVGQSFAFAPIVMYL
                     AYDKLFLVLPLAVLVGLFAVAVVGLINGLITVYLGVSSLITTLGMFFLLAGLNVTLTG
                     GFPATTPETSPVTQVLGEYSWSGTIWALVIVAVLQIVLLRTRWGLHTYATGGNPLGAR
                     EAGVSIARIKIGNFILCGVLGGFVGIIESFRINSIDPLAGGPDIVLLCIAAAVIGGTA
                     LLGGVGTVTGAFLGALVLTILRNGFTLQGISVYTYNIILGVAILAAMILNVYVARLRK
                     AGKV"
     gene            1716..2495
                     /locus_tag="VHM69_06825"
     CDS             1716..2495
                     /locus_tag="VHM69_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2740143.1"
                     /translation="MMANGSDFLRVEGIVKRFGAVEVLKGVDLHVEEGEVLGLIGDNG
                     AGKSTLLKTITGFHRPDEGRILIEGEEVNLRSVSQARSMGIQTVYQDLALVNELSVFH
                     NMFLNGEMTVGPFLNNRKMKEKTTQYLDDMGVNIPSIDTTVARLSGGQRQAIAVARAV
                     YSDAKMLLLDEPLAAMGAKEGALILDLIQRLKEERGIPMILIVHNYAQVFDVCDRVNL
                     LRNGRIEYDKPVAESSVEELTELVVSEYRKARQGNSGNGQR"
     gene            2687..3841
                     /locus_tag="VHM69_06830"
     CDS             2687..3841
                     /locus_tag="VHM69_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2740144.1"
                     /translation="MSNPTASDRTIERTVYTAIVLAFVLVVGAYFVYKISGVVLAFLL
                     TILLSIILSAPVNYLVRRGLPRGWGVLTVIVALAVLLWLFGLALVPAVETQSRQFAEA
                     FPTLLEEALALANQVQSFFGLGTRIGLDPKSLAGIGREVLTGSTVSTAAGVGLTVATA
                     LSLGVVVLISTVYLVIRPEPWVNGFVSLFPAEWRPRAREVLQEMYNTVQRWFIGQLAA
                     MTFIAVFWAISLSVIGIPFALLIGIFSGLLAFIPYVGALISVVIPVLLALISDPFSAI
                     WVILAFVIIQQIEGNLLQPIVMSRAVDLHPALVVFAILVMGTLFGLVGVLLAVPLMAA
                     LQVLVRELWVKRMDRTGTDPDPPSQEPPRAKLPDPIRKFLSTLQGKRLRP"
     gene            complement(3875..4084)
                     /locus_tag="VHM69_06835"
     CDS             complement(3875..4084)
                     /locus_tag="VHM69_06835"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEX2740145.1"
                     /translation="MRDPALAAVLSAIIPGVGQFYNGRILAGILWLIITPGLWIGTGG
                     LLGWTAHVISAYTAYSYARDNPVRT"
     gene            4544..4774
                     /locus_tag="VHM69_06840"
     CDS             4544..4774
                     /locus_tag="VHM69_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740146.1"
                     /translation="MIARAVLCHCRQRLVADDVETLHVLVREHLEQEHPSIPPSAEQV
                     REIVSTRAYDLQYAELYVGRDGITEDFGPEPY"
     gene            4795..5028
                     /locus_tag="VHM69_06845"
     CDS             4795..5028
                     /locus_tag="VHM69_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740147.1"
                     /translation="MSREQTVREMVERVLLRQARRLADRTGMSLDDALQTVAATEAGV
                     QLRELGEGEHRHEQASHWQANLRLERVSEWGRS"
     gene            5114..5320
                     /locus_tag="VHM69_06850"
     CDS             5114..5320
                     /locus_tag="VHM69_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740148.1"
                     /translation="MNSEEALVGVRVMVREEHRSAHLRGQKGTIKHKWGAPDYAALDV
                     LLDNGRLQLFWFHELQAIENQYPI"
     gene            5363..5605
                     /locus_tag="VHM69_06855"
     CDS             5363..5605
                     /locus_tag="VHM69_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740149.1"
                     /translation="MDTQAAPESGTSERQVEPNGRHRAPVVGYYLDESDPDIMILRRR
                     DSTFVAAFSASGATREGICEAAENDYYRASGDGPSS"
     gene            complement(5724..6725)
                     /locus_tag="VHM69_06860"
     CDS             complement(5724..6725)
                     /locus_tag="VHM69_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX2740150.1"
                     /translation="MYGYRPTTRLLSMLELLQARGRMGGPELARRLEVGERTVRRYVA
                     MLQEMGVPVEAERGRYGAYSLRPGFTLPPMMFTDEEAIALALGLLSARRLGLSGAAPA
                     VEGAQAKLERVMPEGLRDRVRTFEEVVVPAAVAPARLPAGDIVVTVSAAVRERRRVKM
                     RYRSGTSEETERVVDPYVVVQGDAFWYTLGYCHLRRGIRLFRLDRVLAVERIDEKFEL
                     PPGFDTPQGVLDALADMREDRWSVEVLLETTVGEASGQLPKMGVSLQETPEGVVMRSS
                     TSDLGWMARVLAGLSFPFVVRRPSELRQSLRRLAAEVAALAERTEDPGHQEAGKGAI"
     gene            6825..7046
                     /locus_tag="VHM69_06865"
     CDS             6825..7046
                     /locus_tag="VHM69_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740151.1"
                     /translation="MRGVHEWFSVELSRERNASMLAEAEERRLARLVPRRSVLRARVA
                     RRLFAFAVALESEETWRAVWERLEAPRHP"
     gene            complement(7084..8295)
                     /locus_tag="VHM69_06870"
     CDS             complement(7084..8295)
                     /locus_tag="VHM69_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX2740152.1"
                     /translation="MKISDVKTICTAPEGIRLVVVKVETDEPGLYGLGCATFTQRPLA
                     VVTAVEEYLKPFLIGRDPDEIEDIYQSAYVSSYWRGGPVLNNALSGVDMALWDIKGKK
                     AGMPVYQLFGGRCRNAVSLYAHATGRDFSEVEDEARRYMEEGYRYVRCQISVPGYSTY
                     GSTISAGPQPLGLGDDPWEPTPYCRLIPRLFEHLRNSLGDEVELLHDVHERIPPIQAI
                     GLARELEPYHLFFLEDPFAPEDVDYFRLLRQRTDTPVAMGELFVNQAEYVPLIRERLI
                     DFIRVHISDIGGLSMARKLAALCEFFGVRTAWHGPGDVSPVGHAANLQLDLACHNFGV
                     QEQHVFDEITREVFPGCPEIRDGYMWPSELPGLGVDIDEELAAEYPFPEDPLNGGWPP
                     VRRLDGTVIRP"
BASE COUNT         1591 a         2685 c         2612 g         1538 t
ORIGIN      
        1 aacggcatcc ctgtcctcgc ctacaacgcc aacggcaagg gaccaggaac gaatccggcg
       61 ctggcttacg tcgggcagga tctcttccag tccggcgtgg agatgggcaa caggatagtc
      121 gagctcgtcg acgaggggct cgtggctctc ttcatagcga cgccgggcca gctcaacatc
      181 cagcctcgca tagatggcgc caagcaggcc atagaggact cgggggcgaa catcgagatc
      241 cagcaggtca ccacgggcgc ggagttgcct gaggagaggt cgaggatcga ggcctggtac
      301 aacggtcacc aggacgttgc ggggatgttc gccgtcgacg cggggagcac ccagggcgtc
      361 gcccaggtga tggagcagtt cggcctgcac gacaagggcg tcaaggcggg tgggtacgac
      421 cttctgcctg agatactcaa gctcatgaag gcggggcaca tagatttcac gatcgaccag
      481 cagccctacc tgcagggctt cttccccgtc atgcagctct acctctacaa tctctccggc
      541 ggggtaacgg gacctgcgga gaccaatacg ggcctcatct tcatcacgcc agacacggtg
      601 gaccgctacc tcgagaccac gtcgcgcttc gaaggggact cggaggcgca gaagatcgtg
      661 aaggcgccct cgtaacctcg cgaagagcgg gaggaggaaa acttgagcga gaccaccgcg
      721 caggagacgg caagtaccgg aacacaggag cgcggtttcg gcggcaggct gatggacagc
      781 ctggtcaggg tgcgggaggt gagcatcctc atcgtcctcg tggcgctggt gctctacttc
      841 caggcggcga acagtgcctt cctctcggag cagaacatcc tctcgctctc ccagtacgcc
      901 gttccgtacg tggtcatcgc gtcgtcgttg tgtttgctgc tcatctgcgg tgagatcgac
      961 ctctccgtcg gacagtcctt cgcgttcgcg cccatcgtca tgtacctggc ctacgacaag
     1021 ctcttcctgg tcctgccgct cgcggtcctc gtcggactct tcgccgtggc ggtagtcggc
     1081 ctgataaacg gtctgatcac cgtctacctc ggcgtgtcct cgctgattac gacgctaggg
     1141 atgttcttcc ttctcgctgg tttgaacgtc acgctcacgg gcggtttccc cgcgaccacg
     1201 cccgagacga gccccgtcac ccaggtcctt ggagagtact cgtggtcggg gaccatatgg
     1261 gcgctcgtca tcgtggccgt tttgcagatc gtccttctgc gcaccaggtg gggtctccac
     1321 acctacgcta cgggcggcaa cccgctcggg gcgagggagg cgggcgtgag catcgcccgc
     1381 atcaagatcg gcaacttcat cctgtgcggc gtgctaggag gtttcgtcgg cattatcgag
     1441 tctttccgca taaactccat agacccgctc gcgggcggac ctgacatcgt tctcctttgc
     1501 atcgccgcgg cggtcatcgg tgggactgcg ctcctcggcg gagtcggcac cgtcacaggc
     1561 gcgttcctcg gtgcgctggt gctcaccatc ctccgcaacg gcttcaccct gcagggcatc
     1621 agcgtctaca cctacaacat catcctcgga gtcgccatac tcgccgccat gatcctcaac
     1681 gtctacgtcg cccgcctgcg caaggcaggg aaagtatgat ggcgaacggc tcggacttcc
     1741 tgcgggtcga aggcatcgtc aagcgcttcg gcgccgtcga ggtcctcaag ggcgttgacc
     1801 tgcacgtcga ggaaggtgag gtgctcgggc tcatcgggga caacggggca ggcaagagca
     1861 cgctcctcaa gacaataacg ggcttccatc gtcctgacga aggaaggatc ctgatcgagg
     1921 gcgaagaggt caacctccgc tccgtctccc aggcgcgctc catgggcatc cagaccgtct
     1981 accaggacct cgccctagtc aatgaattat ccgtcttcca caacatgttc ctgaacggcg
     2041 agatgaccgt gggaccgttc ctcaacaacc gcaagatgaa ggagaagacg acccagtacc
     2101 tcgacgacat gggcgtgaac atcccgtcca tagacaccac ggtcgccagg ctctcgggtg
     2161 ggcagcgaca ggcaatagcg gtggcgcgag cagtctactc ggacgcgaag atgctcctgc
     2221 tcgacgagcc gctcgcggcg atgggggcca aggagggagc cctgatcctc gacctcatcc
     2281 agcgcctcaa agaggaacgg ggcataccca tgatcctcat cgtccacaac tacgcccagg
     2341 tcttcgacgt ctgcgacagg gtcaacctgc tcagaaacgg ccgcatagag tacgacaaac
     2401 cggtcgccga aagctccgtc gaggaactca ccgagctcgt agtctccgag taccgcaagg
     2461 cccgccaggg taactcaggt aacggacagc gctaggccgg acgcttgggt tgtgctcctc
     2521 gcgcatctca tggagcaccg tgagagacgg gctctctacg gttcgcgata aggatggaac
     2581 ccctgcccca cgagcgtagc gtggagaccc ccgtatgtct cttggcggca ccaattgccc
     2641 ggagccgcta aactcaaacc gtacgagcat ccgtaggagc aacgtaatgt caaaccccac
     2701 agcgtcggat cgcacgatag agcgcacggt atacacggcc atcgtgctgg ccttcgtgct
     2761 ggtcgtgggt gcgtattttg tctacaagat cagcggcgtg gtgctcgcgt tcttgctgac
     2821 catcctcctc tcgatcatac tcagcgctcc ggtcaattac ctcgtccggc gagggttgcc
     2881 gcgggggtgg ggagttctga cggtgatcgt ggcgctcgcc gtattgctct ggctctttgg
     2941 tctggcgctc gtccccgccg tcgagacaca atcccgccag ttcgcggagg cttttccgac
     3001 gctccttgaa gaagcgctcg ccctcgccaa ccaggtccag agcttcttcg ggctgggtac
     3061 caggatcggc ctcgacccca aaagcctggc aggcatcggg cgagaggtcc tcacggggag
     3121 cacggtatcg accgcggcag gggtgggact gacggttgcg accgcgctct cgctcggggt
     3181 ggtggtgctc atctccaccg tctacctggt aatccggcca gaaccctggg tcaacgggtt
     3241 cgtctcgctg ttcccagcag agtggcgtcc tagggcgcgg gaagtgctgc aggagatgta
     3301 caacacggta cagcgctggt tcatcggtca gctcgcggcc atgacgttca tcgccgtctt
     3361 ctgggctata tcactctccg tgatcgggat accttttgcc ctgctcattg gtatctttag
     3421 cggtctgctc gcgtttatcc cttacgtggg tgcgctcatc tcggtcgtca tccccgtgct
     3481 tctggccctg atctcagacc ctttctcagc gatctgggta atcctggcct ttgtcatcat
     3541 ccagcagata gagggcaacc tcctccagcc tatagtgatg agccgggccg tcgacctgca
     3601 tcccgccctg gtggtcttcg cgatcctcgt gatgggcact ctcttcggcc tcgtgggggt
     3661 cttgctggcc gttccgctta tggccgcctt gcaggtcctc gtgcgcgagc tctgggtgaa
     3721 gaggatggac cgtacaggaa ccgaccccga cccgccctct caagaacccc cgagagccaa
     3781 acttcccgac ccgatcagaa agttcctgag caccctacag ggaaagaggt tgagaccgta
     3841 ggccggatgg acccgacccc tgaattgcgt agcttcaggt gcgaaccggg ttgtcgcggg
     3901 cgtagctata ggcagtgtag gcagaaatga cgtgcgcggt ccagccgagc aggccacccg
     3961 taccgatcca caaacccggc gtgatgatga gccacaagat tccggccagg atgcggccgt
     4021 tatagaactg tcccactccg gggatgatcg cactcagaac cgccgctaat gctggatccc
     4081 tcaccgtcta ttcacctccg tgctctcgcg aagcgcatac ctctagccgt gcgattacac
     4141 cgatattcta tcgcagcagg gcgaccgcta gaccggacaa atgacctcac agcgcagtac
     4201 ataagtattg acccacctcg ccccagaggt gcaccttagc gaccaccgtc agtcagggaa
     4261 gaccttcctg atgcctgaaa cctgttgccc ggcttgtggg aggatcattc cttccccaag
     4321 ccgctgtcag gcgaccgatg ggtacgatga aggagaaccg cttcctcgtc cggcggtgtt
     4381 ttccaccgca cccgacgctc tcgtccatcg cgccggagtg gctcgacgcc atgtcgaaat
     4441 aagggatcgt caggcactct cacgcactta gtatcctgag acaccgaatt acgccacggc
     4501 catgtgatag gaaggtgtat gggaagaggg aaaggaggag cttatgatcg cgcgtgcggt
     4561 gctatgccac tgccgtcagc gtctggtagc cgacgacgtg gaaacccttc acgtcctggt
     4621 tcgcgagcac cttgaacaag aacacccatc catcccgcca tccgcagagc aggtcaggga
     4681 gatagtctcc acccgtgcct acgacctcca gtacgcggaa ttgtatgtgg ggagagacgg
     4741 tataaccgaa gacttcggtc cggagcccta ttgagtcgct ctggagagct ccatatgagc
     4801 cgggagcaga ccgtccgtga gatggtcgag cgagttctcc tgaggcaagc acggaggctg
     4861 gcggaccgga ccgggatgtc cctcgacgac gcgctgcaga ccgtagccgc caccgaggcc
     4921 ggtgtgcaac tcagggaatt gggcgaaggg gagcacagac acgagcaggc gagccactgg
     4981 caagccaacc tcaggctcga gcgcgtcagc gagtggggca ggtcatgacg tcaaggagaa
     5041 gccgtccgga ttcctctgga ccagtccggc ttcagaagac atcagaagga gagccggtga
     5101 gagagtattc ctcatgaact ccgaagaggc gctggtcggt gtgcgggtga tggtgcgcga
     5161 agagcaccgc tccgcgcact tgcgggggca gaagggcacg atcaagcata aatggggcgc
     5221 ccccgactac gcggccctgg acgtgttgct ggacaatgga cgtttgcaac tgttctggtt
     5281 ccacgagctg caggcgatcg agaaccagta tcccatctag aacgattgcg cgcgtaacgg
     5341 gtctcggaaa ggaggacatt acatggatac tcaggccgca ccggaatccg gcacgtccga
     5401 gagacaagta gagccgaacg gtagacacag agcacccgtc gtgggctatt acctggacga
     5461 gtcagacccg gacatcatga tcctgcgtcg tcgggacagc actttcgtgg cggccttcag
     5521 cgcgagcgga gccacgagag aaggcatctg cgaggccgcg gagaacgact actatcgcgc
     5581 ctcaggcgac ggaccctcca gttaaccggc cggatgggca acttagtccg gttgagttcg
     5641 cgccgccgga caagtcacct tccgggcgaa ccgtcttcgg cttgcgcagg ccgcttgcat
     5701 agccaccttc tcccccaaat agatcagatc gcccctttgc ctgcctcctg atggccggga
     5761 tcttcggtac gctcagcgag cgccgcgacc tccgccgcga ggcgtcgcag cgactggcgc
     5821 aactcggatg gcctgcgtac cacgaagggg aaggagagac cggcaagcac ccgcgccatc
     5881 caccccaggt ccgaggtcga ggaacgcatg acgacacctt cgggcgtctc ctggagcgag
     5941 acccccatct tcgggagttg tccgctggcc tcccccaccg tcgtctcgag taacacctcc
     6001 acggaccagc ggtcctccct catgtccgcg agcgcatcca gcaccccctg aggagtgtcg
     6061 aagccgggcg gtagctcgaa cttttcatcg atcctttcca ccgcgagcac cctgtccagc
     6121 ctgaagaggc gaattcctct gcgtaggtgg cagtaaccga gtgtgtacca gaacgcatcc
     6181 ccctgtacga cgacgtaggg atccacgacc cgctccgtct cctcggaggt cccggaccgg
     6241 tagcgcatct ttacccgtct ccgctcccgc acggcggcgc tcaccgtcac gacgatatcc
     6301 ccggcaggaa ggcgcgcggg ggcgacagca gcagggacaa cgacctcctc gaaggtccgc
     6361 acccgatcgc gcaacccctc gggcatgacc cgctccagct tcgcctgggc gccctctacg
     6421 gcaggggccg cgcccgagag gccgagcctg cgggccgaga gcagcccgag agcgagcgcg
     6481 atggcctcct cgtcggtgaa catcatcggc ggtagcgtga agcccggcct caaagagtac
     6541 gcgccgtacc tgccccgctc ggcctcgacg ggaacaccca tctcctgcag catcgccacg
     6601 taacggcgca cggtcctctc tccgacctcg agcctcctcg caagctcggg accgcccatc
     6661 ctcccgcgcg cctggagcag ctccagcatc gagagcagcc tcgtcgtcgg gcggtaaccg
     6721 tacaccctct ctctacctcc gaaaaacctc gcctctctac aggttatcag gaaggattat
     6781 gaccgctttc gccggtactc tcatcaggta ggcgagacag ggagatgcgt ggcgtgcatg
     6841 agtggttttc tgtggagctc tctcgggaga ggaacgccag tatgctggct gaggcggagg
     6901 agcggcggct ggcgcgtctg gtcccaagga ggtcggtact gcgggctcgg gtagcgcgga
     6961 gacttttcgc gttcgcggtg gcattggaaa gtgaagagac ctggcgggct gtctgggaga
     7021 ggctggaggc gccgcggcac ccgtgagtcg caggcgtctc gcgcaactcc gcggtctgcc
     7081 cgttcaagga cggatcacgg tcccgtccag acgcctgaca ggcggccatc ccccgttcag
     7141 tgggtcctcg gggaacgggt actccgccgc gagttcctcg tcgatatcga cgccgagacc
     7201 gggcaactct gaaggccaca tgtagccgtc acggatctca gggcatccgg ggaagacttc
     7261 gcgggtgatc tcgtcgaaga cgtgctgctc ctgtaccccg aagttgtggc acgcaaggtc
     7321 gagctgcagg ttggcggcgt ggcccacagg tgagacgtct ccgggcccgt gccaggctgt
     7381 gcgtacaccg aagaactcgc agagggccgc cagcttgcgg gccatgctca ggccgccgat
     7441 gtccgagata tgcacccgga taaagtcgat cagtcgttcc ctgatgagcg gcacgtactc
     7501 ggcctggttg acgaacagct cccccatcgc gaccggcgta tcggtccgct ggcgcaggag
     7561 ccggaaatag tccacgtctt ccggggcgaa agggtcctcg aggaagaaca gatggtaggg
     7621 ttccaactct ctggcgagac ctatcgcctg gatcggcggt atgcgctcgt gtacgtcgtg
     7681 gagcaattcg acctcgtcac ccaaggagtt tcgcaggtgc tcgaagaggc gggggatcaa
     7741 tcggcagtaa ggcgtcggct cccacgggtc gtctcccagt ccgagcggct gcggtccggc
     7801 tgagatcgtg gacccgtacg tggagtagcc gggaacggag atctggcacc gcacgtacct
     7861 gtaaccctcc tccatgtacc tgcgcgcctc gtcctcgacc tcgctgaagt cccgtcctgt
     7921 agcgtgggca tagagggaaa ccgcgttccg gcacctgccg ccgaagagct ggtacacggg
     7981 catgcccgcc ttcttgccct ttatgtccca cagggccatg tccacgcccg agagggcgtt
     8041 gttcaggacg gggccgccgc gccagtacga actcacgtag gctgactggt agatatcctc
     8101 gatctcgtcg gggtcgcgtc cgatgaggaa cggcttcaga tactcctcga cggccgtgac
     8161 caccgcgagc gggcgctggg tgaaggtggc gcacccgagg ccgtagagtc caggctcgtc
     8221 cgtctcgacc ttcacaacca cgagcctgat gccctcgggt gcggtgcaga tggtctttac
     8281 gtcggagatc ttcaattgcc tgccctcgta aggacgcttt caagcgccgc ttcggggtcg
     8341 tagtccgccg ggacgggcac gaagggcggc cggacggtcc ccatcggcgt cccgtgcctt
     8401 gccccagcga gctttttgag gaccgc
//
LOCUS       DATAIR010000279         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110528, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000279 DATAIR010000000
VERSION     DATAIR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110528"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VHM69_06875"
     CDS             <1..1155
                     /locus_tag="VHM69_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2740153.1"
                     /translation="VEAGGQGAIMAPTEVLAEQHYLSISAALAELPVNVVLLTGSQGA
                     SERRAALEQIAGGGAHVVVGTHALIQKGVDFKDLSLVVVDEQHRFGVGQRTVFREKGT
                     TPDTLVMTATPIPRTLSLTLYGDLEVSIIDELPPGRKPVETRIVDPAERLGAYNEVRL
                     EMEDGRQAYVICPLVEESEALEDVRAAEELREELAEEVFPERRVGLLHGRMKAQDKRE
                     VMASFRAGEVDVLVATVVVEVGVDVPNASAIVIEGSERFGLSQLHQLRGRVCRGLHSP
                     KCFLVGAPKTDDSERRLQALVEYQDGFKLSEVDLAIRGEGTLFGSRQSGMPDLKVAKL
                     LRDVDVLVEARREAFSLVAGDPTLKRPDHRPLRREIRELLGANVEWLFRE"
     gene            1152..2186
                     /gene="coaD"
                     /locus_tag="VHM69_06880"
     CDS             1152..2186
                     /gene="coaD"
                     /locus_tag="VHM69_06880"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2740154.1"
                     /translation="MRVISGSARGRRLAPVPKNVRPTSDRVRESLFNSLGQYFEGGSV
                     LDLYAGTGALGIEALSRGSERAVFVERDRRVRAVIHENLRRTGFVEQAEVIGDDVESV
                     LGRLVERGELFNLIFADPPYRIAAAEVGGILGRLRSLLTPGGRVVIESGEAPVAGVEK
                     GVTRRYGGTFLTILERSELTMIVAVCPGSFDPVTTGHLDIIRRAAKIFDHVVVAVGSN
                     MRKQSRLPAVERARLIEKVTADMDNVSIEVMEGLLVDFAREQGAQAIVKGLRAVSDFE
                     SEFEQAQLNRTMFPELETVFIMASAEHSFLSSSAVREIASFGGEVRGLVPDGILETVR
                     QIYSGSDGKI"
BASE COUNT          446 a          678 c          814 g          424 t
ORIGIN      
        1 gtcgaggcag gggggcaggg tgcgatcatg gcccccaccg aggtcctcgc cgagcaacat
       61 tacctctcga tctcagcggc tttggccgaa ttgccggtca acgtcgtgct cctcacaggc
      121 tcgcagggag cttcagagag acgagccgcc ctcgagcaga tagcgggcgg cggggcccac
      181 gtcgtcgtag gtacgcacgc cctcatacag aagggcgtcg atttcaaaga cctctccctc
      241 gtcgtcgtgg acgagcaaca ccgcttcggg gtcggccagc ggacggtgtt ccgcgagaaa
      301 gggacgaccc ccgatacgct cgtcatgacg gcgactccta taccgcggac actttcattg
      361 acgctatacg gcgacctcga agtctccata atcgacgagt tgccgcctgg acgaaagcct
      421 gtcgagacga ggatcgtcga ccccgctgag cggctcgggg cgtacaatga ggtcaggctg
      481 gagatggagg acgggaggca ggcgtacgtg atctgccctc tcgtcgagga gtccgaagcc
      541 cttgaagacg tgcgggcggc tgaggagctg cgcgaggagc tggccgagga ggtcttcccc
      601 gagagaaggg tcgggctcct gcacggcagg atgaaggcgc aggacaaacg ggaggtcatg
      661 gcatctttcc gcgctgggga ggtcgacgtg ctcgtcgcta ccgtggtcgt cgaggtcggt
      721 gtcgacgtgc ccaacgcgag cgcgatcgtg atcgaaggct cagagcgctt cggcctctcc
      781 cagctccacc agctaagggg ccgcgtgtgc cgggggctcc attccccgaa gtgtttcctc
      841 gtcggcgccc cgaagaccga tgactccgag cgccgcctcc aagcgctggt ggagtaccag
      901 gacggcttca agctctccga ggtcgacctc gcgatccgtg gcgagggcac cctcttcggg
      961 agccgccaga gcggcatgcc tgacctcaaa gtagccaagc tgctgcgcga cgtggacgtc
     1021 ctggtcgagg caaggcgcga ggctttctcg ctcgtggccg gggatcccac actaaagcgt
     1081 cccgaccaca ggcccctgcg gcgtgagatc cgggagctcc ttggcgcgaa cgtcgagtgg
     1141 ctcttcaggg agtgagggtc atatccgggt ctgcccgcgg taggaggctc gcccccgtcc
     1201 cgaagaacgt gcgtcctacc tctgaccgcg tgcgggagag cctgttcaac tcattagggc
     1261 agtacttcga aggcggcagc gtgctcgacc tgtacgcggg gacgggcgcg ctcgggatag
     1321 aggcactcag ccgcgggagc gagcgggccg tcttcgtcga gcgcgacagg cgggtgcggg
     1381 ctgtgatcca cgagaacctc cggaggactg gcttcgtgga gcaggccgag gtgatcggtg
     1441 acgacgtcga atccgtgctc gggcggctcg tcgagcgtgg tgaactattc aatttgatct
     1501 tcgcggaccc accatataga atcgctgcgg cggaggtcgg tggtatcttg ggccggctac
     1561 gttctctgct cacgcccgga ggaagggtcg tgatcgagag cggcgaagct ccggttgcag
     1621 gtgtcgagaa aggggtgacg cgccgctacg gcggaacgtt tttaaccata cttgagcggt
     1681 ccgagctgac aatgatcgtg gctgtgtgtc ccggtagctt cgaccccgtt acgacggggc
     1741 acctggatat catccggcgt gccgccaaga tcttcgacca cgtcgtcgtg gctgtgggta
     1801 gcaatatgcg caagcaatct cgtctgcctg cggtcgaacg ggcgcggctc atagagaagg
     1861 tgaccgccga catggacaat gtgtccatcg aggtgatgga aggtttactg gtagatttcg
     1921 ccagggaaca gggtgcgcag gcgatcgtga agggcttgcg ggccgtctcg gatttcgagt
     1981 ctgagttcga gcaggcgcag ctcaacagga ccatgttccc cgagctcgaa acggtcttca
     2041 taatggcctc cgcggagcac agcttcctct cttcgagcgc ggtcagggag atcgcttcgt
     2101 tcggagggga ggtgcgcggg ctcgtgccgg acggcatact tgagaccgta aggcagatat
     2161 attcaggttc ggacggtaag atatagggat cgtaggcaaa ggataggtag tagaagatgg
     2221 acgtactggt actcatagac aggttggaag aactagtcga ggacgcgcgg agcttccccg
     2281 gtttcggaaa cacggcgatg atcgaccggg acgcagcctt cgacatcata gaccagatgc
     2341 gccagaccat acccgaggag ct
//
LOCUS       DATAIR010000280         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110561, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000280 DATAIR010000000
VERSION     DATAIR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110561"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..707
                     /locus_tag="VHM69_06885"
     CDS             75..707
                     /locus_tag="VHM69_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX2740155.1"
                     /translation="MNRVGRRLSKEEKGFTLVEVLVAATMMFAMLIALYAIFDVSVRA
                     FGYGGERLEAVGNARLAMSKMEREIKAAHPYDLTSDPQKRHLFLNQANPAQPAVPTAT
                     RITFGNEVGGDRRIGASEVIGYYLSGHDLKRSKGGSAQTLLDSVPTDGLRFTPCRSAE
                     ECPPTLAITDEAQVKFVRIELTIEVRRRGEDPTRQTLTTGVHLRNRWEQR"
     gene            737..1117
                     /locus_tag="VHM69_06890"
     CDS             737..1117
                     /locus_tag="VHM69_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740156.1"
                     /translation="MALGLAIIVVVLVGVMASGLLTLVATDLQAVVEANRGEQAFELA
                     EAGIEVAKAHLANAPSAADWSSGELHLDGMGENAVTVTVDHEGGTFEAVSTGQYGQTK
                     RKIEATFSIEDGRPELLSWRELYE"
     gene            complement(1279..1788)
                     /locus_tag="VHM69_06895"
     CDS             complement(1279..1788)
                     /locus_tag="VHM69_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740157.1"
                     /translation="MIIRKDQVPLDRGTEDGTQKYGASESLRYSEAGGLTQFGAYVQT
                     LQPGSRSSDRHWHEEEDEFLYILSGEATVIEEDGAHPLGPGDAACWPASTANAHQVVN
                     RSGAPCTYLIFGTRMARDVVHYPEAGRVLHDEDGRWRLLRTDDGSLIKGGEIEQVRPM
                     TWRGPGVDR"
     gene            complement(1946..2614)
                     /locus_tag="VHM69_06900"
     CDS             complement(1946..2614)
                     /locus_tag="VHM69_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740158.1"
                     /translation="MDRKKVPTPDQRTSARYLLEMPGDRLYSEPRAFPKICSASLFGD
                     EKPMELEVGCGSGEFLCSLAQRDRETNFVGIEVKHKSVYEAVATAASIDLDNILFLRA
                     NFRLLYPLLMTNSLRAVYLHFPDPNTRQKFRKHRIFSDRFLDEMHAATVAEGRISVMT
                     DHAGSFAEMLELAESDDRWEKAHEERYLVGFEDESRSRYQRIWEGHGIPTLRFELVKS
                     GGRG"
     gene            complement(2939..3079)
                     /locus_tag="VHM69_06905"
     CDS             complement(2939..3079)
                     /locus_tag="VHM69_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740159.1"
                     /translation="MALSRSQSVAFGEGVVFAQPIDVLKTRVFRGTTPFRCRLDRRST
                     LW"
     gene            3179..3418
                     /locus_tag="VHM69_06910"
     CDS             3179..3418
                     /locus_tag="VHM69_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740160.1"
                     /translation="MRTRVGGPDDPREGLEAVVALRRMLEALEAAQVENAFVAGWSWA
                     KIAEVLGVSKQAVHKKHARRIKDKYPGEPPGRKRT"
BASE COUNT          751 a         1178 c         1262 g          724 t
ORIGIN      
        1 cttgcgagga tcaccattac ggtgaactgg ggcggcgaga gctcatatag cgttgccggg
       61 gtagtgtcca ggtggtgaac cgcgtcggta gacggctgtc gaaggaggaa aaagggttca
      121 cgctcgtgga ggtactagtc gccgcgacga tgatgttcgc catgctcatc gcgctctacg
      181 cgatcttcga cgttagcgtc agggccttcg gttatggcgg cgaaaggctg gaagcggtcg
      241 ggaacgccag gctcgccatg agcaagatgg agcgtgagat caaggccgcc cacccttacg
      301 acctgacctc ggatccacag aagaggcacc tgttcttgaa ccaggccaac cccgcgcagc
      361 ctgcggtgcc taccgccacc cgcatcacct tcggcaacga ggtcggggga gaccgcagga
      421 tcggcgcctc cgaggtgatc ggctactacc tcagcggcca cgacttgaag cggtcgaagg
      481 gcggcagcgc ccagacgctt ttggattccg tcccgacgga cggtctccgg ttcacgccct
      541 gcaggagcgc ggaagaatgt cctccgactc tggcgattac ggacgaggca caggtcaagt
      601 tcgtccggat agagctgacc atcgaggtca gacgtcgtgg tgaggaccca acccgtcaga
      661 cgctgacgac gggtgtccat ctgaggaacc gctgggaaca acggtgacct gccacctcct
      721 gcgcgaagag aggggtatgg ctctcgggct cgctattatc gtggtcgtgc tcgtcggggt
      781 gatggcctcc ggcctcctga ccttggtggc cacggacctt caggcagtcg tggaagcaaa
      841 caggggagag caggccttcg agctggccga agcgggcatc gaggtcgcga aggcgcacct
      901 cgcgaacgcc cccagcgccg ccgactggtc ttccggggaa ctccacttgg acgggatggg
      961 tgagaatgcc gtcaccgtca ccgtcgatca cgagggcgga actttcgagg ctgtctctac
     1021 gggccagtac ggtcagacga agcgcaagat cgaagctacc ttttccatcg aagacggcag
     1081 gccagagcta ctgagctggc gtgagctata cgagtagtcg gtcttagacc cacgatgagc
     1141 gatgtgtgcc gtggccagaa tcagcacagg tcccaccaga gttgctcatg cactctgcga
     1201 agcatggctg tcgcgacgct gagaagactg cgggggattc tacggcctca cggtcgtcct
     1261 cctgattcgg tcctcgggct accggtcgac ccctggaccc cgccatgtca tggggcgaac
     1321 ctgttcgatc tctccacctt tgatcaggga gccgtcgtcg gtacgcagta gccgccatct
     1381 tccgtcctcg tcgtgcagga cccgtcccgc ttcggggtag tgaacgacgt cgcgcgccat
     1441 ccgggtgccg aagatgaggt aggtgcaggg cgcgcccgaa cggttcacga cctggtgggc
     1501 gttcgccgtg ctcgctggcc agcaggccgc atcgccagga ccgagcggat gggcaccgtc
     1561 ctcctcgatc acggtcgctt cgccagaaag tatatagagg aactcgtcct cctcttcgtg
     1621 ccagtgccgg tcggaagagc gtgagcccgg ttgcagcgtc tggacgtagg cgccgaactg
     1681 cgtcaggccg cccgcttcgg aataccgcaa tgactcggag gcgccgtact tctgtgtgcc
     1741 gtcctcggtc ccccgatcca gcggtacctg gtcttttcgg atgatcatct cggtcctccc
     1801 aagacgtgac tttaggtggt ctcaactgtg cccgcctcga agtacccgcg caaggtgctg
     1861 gtccgacgct atgaggcagc gcaccatcaa gcccgcggca acaggtctat ggcccgcgct
     1921 gccgtcggga gagagtggcc ccaggctacc cgcggccccc gctcttcacg agctcgaatc
     1981 gcagcgtggg tatcccgtgt ccctcccaga tacgctggta ccttgaccgg ctttcgtcct
     2041 cgaatcccac caggtagcgc tcctcatggg ccttttccca gcgatcgtcg ctttctgcca
     2101 gctccagcat ctcagcgaaa gaccctgcgt ggtcggtcat aacgctgatc ctcccttcag
     2161 cgaccgtggc ggcgtgcatc tcgtccagga agcggtcgga gaagatccgg tgcttccgaa
     2221 acttctggcg cgtgttgggg tcggggaagt ggaggtagac ggcgcgaagc gagttcgtca
     2281 tgagcaaagg gtagaggagc ctgaagttgg ccctgaggaa caggatgtta tctaaatcta
     2341 tggaagcggc cgtggcgaca gcctcgtaga cggacttgtg tttcacctcg atgccgacga
     2401 agttcgtctc gcggtctctt tgggccagcg agcagaggaa ctccccgctc ccacagccta
     2461 cctccagctc catgggtttt tcgtcgccga acagcgaggc agagcagatc ttcgggaagg
     2521 cccgcggctc gctgtagagc cggtccccgg gcatctcgag caagtacctg gcggacgtcc
     2581 tttggtcggg cgtgggaacc ttcttgcggt ccaaactcct tcttggcatg cgcgttcccc
     2641 ttgctccccc cgcaggggga tctagagagg ggcagaccgc ggacggatgg gtggcgcggc
     2701 ccgattcgcg cggaggtcgg gaggccccga gccgggaggt tcctacgaat tgccttgaaa
     2761 cggggctacg ccggggcgca aaaggagagg ccgcgggcgc aaggggagcg cccgcggcct
     2821 gccgggtaaa ccagaaaccc taccttgagc ggacgaactc ctcctgccgg aaccgcgaaa
     2881 ccgtcttaac gtttaccggg ttggcggagt tctgattctc gctcatcacc caggcaagct
     2941 accagagtgt cgagcgccgg tcaagacggc atctgaacgg cgtggtgccg cgaaagacgc
     3001 gggtcttcaa aacgtctatc ggctgggcga agacgacgcc ctcgccgaag gcaaccgact
     3061 gggacctcga aagcgccatg ccgggccgcc cgaactgccg tgagggaaaa gtcgagcaaa
     3121 tatgtcaata atagttgaca aatacaatat cgtcaactat tattgacgca tggacaggat
     3181 gagaacacgg gtaggtgggc cggacgatcc gcgggagggt ctcgaagccg tcgtcgcctt
     3241 gaggcggatg ctggaggcgc tcgaggcggc gcaggttgag aacgctttcg tggctgggtg
     3301 gtcgtgggcg aagatcgccg aggtgttggg ggtgagcaaa caggcggtgc acaaaaagca
     3361 cgccaggagg atcaaggaca agtaccctgg agaaccgccc gggaggaagc gcacatgacg
     3421 tttggccgtc tgttacgcgc tagccggttg tcccgccggc tcgtccgcat ctatcgatca
     3481 cacctcctct tcgctctctc ctcgttacgg ggcggccaac cggcgtcatc aacgtgaagg
     3541 gaggaggagt aaaaacacat tgtgacgcca ggttgggccg ctgcggctct gccgcgctct
     3601 tctcaggcaa cctctagggg tcaaccccga agtgcgcgct tcgagagccc cacatcgccc
     3661 ctcttccggg gcgacctcct ttgttgtcga gagtatcaag ctggagagtc atcgagcatc
     3721 gtccgaatga ctcgtttttg agtgagtcat attctctatt cttccgggtc acgaacgctc
     3781 taacgggaaa tgaggtatgt gcgcgatgcg ccccgctggt ggtctctgcc accatctctc
     3841 atgacgcaaa acggccaacc gtcaaggagg cgacaagcat ggtcgacgag aagctacagg
     3901 agaagctcgc agatt
//
LOCUS       DATAIR010000281         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_295491, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000281 DATAIR010000000
VERSION     DATAIR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295491"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VHM69_06915"
     CDS             complement(<1..894)
                     /locus_tag="VHM69_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740161.1"
                     /translation="MVGGSGRRELLVSRGWVQAVAIVVLFGFFVLGLLAYRTYSGQPP
                     IPERVVDPSGEVVFTGADITAGQGVFLRNGLMQYGSIFGHGAYLGPDFTADYLRRSAL
                     SVRESYGGEGSDRAASRTVEDFKQNRYDARSGTLEFTAPQSAAFEELTRHYGRYFGEP
                     STRYGLRPEAIKDPEEIRQLTAYFAWTSWAASTLRPGLDYTYTNNWPPEPLVENEPTA
                     NVVVWSVLSLIALLGGIGLLFAAFGRWNFLGWRGREQATLSFRAPGDVALTPAQRACA
                     WFFLVMAALFLIQTLVGAASQH"
     gene            complement(1031..1456)
                     /locus_tag="VHM69_06920"
     CDS             complement(1031..1456)
                     /locus_tag="VHM69_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX2740162.1"
                     /translation="MELSSEGRYALRALLYLAWIGERVTAQRISAEAHVPRRLLARIL
                     AKLSHARLVESEQGRGGGSRLARPATEITLREAVEAVEGPFGVTRCILEDRACGEGAP
                     CILHEAWDEGQRSILEYLDTQTLEDFLMQNPSNQLPPLT"
     gene            1649..2008
                     /locus_tag="VHM69_06925"
     CDS             1649..2008
                     /locus_tag="VHM69_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740163.1"
                     /translation="MHGLLQSFDLEAEVSRLREEQEWQKGLRNAITLRKGQGLSVVLV
                     VMKAGDVLDEHSAPGPISLVVREGRVRFTAAEKTVEAGTEALLTCDAGVKHSVEAVSD
                     AVCLLHIAAGETARRSG"
     gene            2021..2362
                     /locus_tag="VHM69_06930"
     CDS             2021..2362
                     /locus_tag="VHM69_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740164.1"
                     /translation="MQENSVNVGEIAASTAGQKGVVWTLDGSVDLNANLVRFGVDGGV
                     EEHVNDEVDVIFVGVSGSGRVEVDGEEYPLSNGTMVFVPRGVKRSVRSTSEDFVYLSV
                     HRRRGPLRIGG"
     gene            complement(2402..3457)
                     /locus_tag="VHM69_06935"
     CDS             complement(2402..3457)
                     /locus_tag="VHM69_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2740165.1"
                     /translation="MEDFHERALEAIEEVFGDRMKSTPPENTGEASGLELASVFPTSA
                     EEVVLLAEVARRYSMPLAALGAETSPDTPVKEGRILVRFDLMRGHQLPDSDEPWAEAE
                     PGALWLELDDQLRLRGRGLTVYPTSAPKATVGGWLAQDGVGVGSFEYGRLSENVVSAD
                     VVTPDGGRRTVEGEELRLVVGPENGGAIVVGARLRTRRAEDDVPYVLAFGDSEDLARA
                     VASILDAGVPLWHLGLLSLQMARLRGLGEEYLLFGAYPSEREALARDALGEVASLNDG
                     RLLPSPDAYRVWGERFFPVMPSHPTPILSDRTFVPVTEISAFLAKRPEMAVQGTVTRS
                     GEVLLLAFDPHEMTMAR"
     gene            3679..3942
                     /locus_tag="VHM69_06940"
     CDS             3679..3942
                     /locus_tag="VHM69_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740166.1"
                     /translation="MAQSADRSIGEHVRRAHEAIRGEHQLAEYVAGLEIEECARILAS
                     DPDGLPRLAALRDECRVGSAEKRSFERVIRTWTEAVLSGIEGG"
     gene            3966..>4257
                     /locus_tag="VHM69_06945"
     CDS             3966..>4257
                     /locus_tag="VHM69_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2740167.1"
                     /translation="MFPTKILLAIDGSPHSELAARMAADFATKTGSELHVVHVGHVPS
                     AYAAAESEILDYEFWKEMREFAKREVSQNLEDEVRKIEGVGGEVEKSHVAVGR"
BASE COUNT          840 a         1350 c         1319 g          748 t
ORIGIN      
        1 atgctgcgag gcagccccaa cgagggtctg tatgagaaac aacgccgcca tgaccaggaa
       61 gaaccacgcg cacgccctct gggccggcgt cagcgccacg tcgccagggg cgcggaacga
      121 cagcgtcgcc tgctcgcgcc cgcgccagcc aaggaagttc caccgtccga aggccgcgaa
      181 cagtagcccg atgccgccaa gcaaggcgat cagggacaga acgctccaaa ccacgacgtt
      241 ggcggtcggc tcgttctcga ccagtggctc gggcggccag ttgttggtgt acgtgtaatc
      301 caacccaggc cgcagggtcg aagccgccca cgaagtccac gcgaagtacg ccgtgagctg
      361 cctgatctcc tccgggtcct tgatcgcttc tggcctgagc ccgtacctgg tgctcggctc
      421 cccgaagtac cgcccgtaat gcctggtgag ctcctcgaag gccgcagact gcggcgccgt
      481 aaactcgagg gtcccgctcc tggcgtcgta gcggttctgc ttgaagtcct ccacagtgcg
      541 cgacgccgcc ctgtccgacc cctctcctcc atagctctcg cgcacggaaa gcgcggagcg
      601 cctcagatag tcggcggtga agtcaggacc caggtaggcg ccgtggccga agatggaacc
      661 gtactgcatg agaccgttcc gtaagaacac cccctgcccc gccgtgatat ccgctccggt
      721 gaagaccacc tcgcccgacg ggtcgactac ccgctccggt atcggcggct gccccgaata
      781 ggtccggtac gccagaaggc cgagcacgaa gaagccgaac aacactacta tcgcaacagc
      841 ctgcacccag cccctggaca caagcaactc ccgacgcccg gaccctccga ccataagcca
      901 cctccacaca acgccggacc agcggatctc ggctcaacta ttacataatc agtaatataa
      961 tcatgagctt ggcgagcgcg caagtattcc aggatacagg ccggacaata cgcgggctgg
     1021 cgtaaaggca ctaggtaagt ggaggcagtt gattggaggg gttctgcatg aggaaatctt
     1081 ccagcgtctg ggtatcgagg tattcgagaa tagagcgctg gccctcgtcc caggcttcgt
     1141 gcaggatgca gggagcgcct tccccgcagg cacgatcttc taatatgcag cgggtgacgc
     1201 cgaacggccc ctccacggct tccacagcct ccctgagcgt tatctcggtc gctggtcgcg
     1261 ccaggcgtga gccgccgccg cggccctgtt cgctttcgac caggcgtgcg tgtgatagct
     1321 tggccaggat gcgggccaga aggcggcgtg gaacgtgggc ctcggcggag atcctctgcg
     1381 ccgtcacccg ctccccgatc caggccaaat acagcagagc cctcaacgcg taccgcccct
     1441 cactgctcaa ctccaaccgc aaatcctgcg ctcctccaca cggtactgaa ccgccgtctt
     1501 ccacctgatc ccattttttc tgaaccgaag attacaggat cagcaagggt tctcccaaat
     1561 ctattactat atttgtcata atatacgagt gttccgagtc aaagggggta agatcttgac
     1621 aggagacagt ccgagatcag gacggcagat gcacggcctg cttcagagct tcgatctcga
     1681 agccgaggtt tcgcggctgc gcgaggagca ggagtggcag aaagggctac gcaacgccat
     1741 tacgctacgg aaaggacagg ggctcagcgt ggtcctggtg gtgatgaagg ctggcgacgt
     1801 actcgatgag cattccgctc ctggtcccat ctcgttggtt gtacgcgagg ggcgcgtacg
     1861 cttcacggcg gcggagaaga ccgtcgaggc tgggacggag gcgctgctca cgtgcgacgc
     1921 gggtgtaaaa cattcggtgg aagcggtgag tgacgccgtt tgtttgctac acatcgctgc
     1981 gggagaaaca gcgcgccgtt caggatagga tatcggccat atgcaggaaa attccgtgaa
     2041 cgtcggcgag atcgccgcct ctactgccgg gcagaaaggg gtggtctgga ctctcgatgg
     2101 gagtgtggat ctcaacgcca acctggtgcg tttcggcgtg gacggcggcg tcgaggagca
     2161 cgtcaacgac gaggtagacg tgatcttcgt cggcgtctcc ggttctggcc gggtcgaagt
     2221 ggacggtgaa gagtaccctt tatcgaacgg aaccatggtc ttcgttcctc gtggcgtcaa
     2281 acgctccgtc cgtagcacgt ccgaggattt cgtgtacctc agcgttcatc gtcgtcgggg
     2341 tccgcttcgg atcggcggtt gagccgaagc ggagcagacg gggatcgcac cttcgccgaa
     2401 cctatcgcgc catggtcatt tcgtgggggt cgaaggcgag cagcaaaacc tcgcccgagc
     2461 gggtgacggt gccctgtacg gccatctccg ggcgcttcgc aaggaaagcg gagatctccg
     2521 taacagggac gaacgtacga tccgatagga tgggcgtggg atgcgagggc atgactggga
     2581 agaacctctc cccccacacc cgatacgcat cgggtgaagg aagcagacgc ccgtcgttga
     2641 gcgaggcgac ctccccgagc gcgtctctgg ccagcgcctc ccgctcgctc gggtatgcgc
     2701 cgaagagcag atattcctcc cctagacccc tgagtcgcgc catctggagg ctcaacaagc
     2761 cgaggtgcca gagcggcacc ccggcgtcga ggatgctcgc caccgcgcgt gccagatcct
     2821 cgctgtctcc gaaggcgagg acgtagggaa cgtcgtcctc ggcgcgcctc gtacggagcc
     2881 tggcgccgac cacgatcgcc ccaccgttct caggtccaac gaccaaccgc agctcctcac
     2941 cctccacggt ccggcgcccg ccgtcgggcg tcaccacgtc ggccgagacc acgttctcgc
     3001 tcagcctgcc gtactcgaag gagcctaccc cgacaccgtc ctgggcgagc catcctccga
     3061 ccgtggcctt cggagcgctc gtcggataga ccgtgagtcc ccgtcctcgg aggcgcagct
     3121 ggtcgtccag ttcgagccac agcgcgccag gttccgcctc agcccagggt tcgtccgagt
     3181 cgggcagctg gtggccgcgc atgaggtcga agcggaccag gatcctcccc tccttcaccg
     3241 gcgtatcggg ggaggtctcg gccccgagcg ctgccagagg catggagtag cgtcttgcga
     3301 cctccgccag aagcactacc tcctccgcgc tcgttgggaa gacggaggca agctcgaggc
     3361 ccgaagcctc tccagtattc tctggcggcg tgctcttcat gcggtcgccg aagacctctt
     3421 cgatggcttc aagtgccctc tcgtgaaaat cttccatctt cgtacacctc actcgtcgat
     3481 tacctttaca gcaagattac cggctcgtac gagctcccgc atgatccatg acgattcaac
     3541 ctgactgaat tgcccaactt cgccctccct ctattggtca atgcgcgcca tgaagatgag
     3601 ccaatttgct caagtcacct ccattctcgg agactacact gtctacgcag gcaagcgatg
     3661 aacggaggga gtaccggcat ggcacaatcg gcagaccgtt ccatcggcga gcacgtcaga
     3721 agggcgcacg aagccattcg tggggaacac cagctcgccg agtacgtcgc cggtctggag
     3781 atcgaagaat gcgcgaggat actggcttcc gatcctgacg gcctcccacg ccttgccgcg
     3841 ctaagagacg agtgccgcgt cggttcggca gagaagaggt cctttgaacg ggtgatccga
     3901 acctggacgg aggccgtgct ctctggcatc gagggcggct gaggtagaaa gggggtacgg
     3961 acgccatgtt cccgacgaag attctgctgg cgatcgatgg tagcccgcat tcggagctgg
     4021 cggcccggat ggcggcggac ttcgcgacga agacgggctc ggagctgcac gtcgtccacg
     4081 taggccacgt gcccagtgcc tacgcagccg cggagtcaga gatcctcgat tacgagttct
     4141 ggaaagagat gcgagagttc gccaagcgtg aggtctcaca gaatctcgaa gacgaggtgc
     4201 gcaagataga gggtgtcggc ggagaggtcg agaagtccca cgtcgcggta ggtcgtc
//
LOCUS       DATAIR010000282         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_433484, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000282 DATAIR010000000
VERSION     DATAIR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433484"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1979
                     /locus_tag="VHM69_06950"
     CDS             318..1979
                     /locus_tag="VHM69_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2740168.1"
                     /translation="MAKPVILAVDDDPQVLRAVERDLKRKYAREYRVLRADSGASALD
                     ALDRLKVRGDPVALFLVDQRMPKMTGVEFLEKAIEVFPEAKRDLLTAYADTDAAIRAI
                     NEVGLDYYLQKPWDPPEENLYPILDDQLEEWHSGFNPPFEGIRVVGDRWSPDSHRLRD
                     FLARNRVPYKWLDVEGSEEARQIVARLDSGSPKLPAVVFQDGTHLEAPENREVAERIG
                     LKTRAERPFYDLIIIGGGPSGLAAAVYGASEGLKTVLIEREAPGGQAGTSSFIENYLG
                     FPQGLKGSELARRAVDQAKKFEVEILTPQEVADVRVEDPYRIVRLGDGTELSCHAMII
                     ATGVSYRKLKAPGVDRLAGAGVYYGASLTEAQSCKNEDVYIVGGANSAGQAAMYFSEH
                     ARKVTILYRGDSLRKSMSEYLVKQVEGKDNIEVRLNSEVIEVEGEGRLERIVVRDSAS
                     GASETVPAQSLFIFIGAAPHTDWLGDRVVRDERGFILSGSDLSRNAERPRGWRLERDP
                     FLLETSVPGIFVAGDVRHGSVKRCASAVGEGSIAVQFVHQYLKEV"
     gene            1981..>3097
                     /locus_tag="VHM69_06955"
     CDS             1981..>3097
                     /locus_tag="VHM69_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2740169.1"
                     /translation="MQGAAVIETLRKVPLFSELPDDGLAWISERGEEVRLDAGTTISA
                     QGDPPDGLYVVLEGETEWTREVAGQEVIVAKLGEASTFAGLFLVLDAPYPTTGRALTP
                     LRLFRLDAPLFWEMLRTFPEVLRGILANCFERPVPRDPVTQQHASLISLGTMAAGLAH
                     ELNNPAAAVTRSAQEARAAFRESISRAIKLGELGLSPAERAFVAGLPDEVASRAETMP
                     ALDSLEQGDREDEVALWLEDRGIDEAWELCSVFVGAGLDTDWLEELADHLPDERALGD
                     VLAWLASVINGDALLREIKQASARVSELVGAVKSYSHMDKASRKEADVQKGLDNTLVM
                     LGHKLKKGNVRVTREYEEDLPTVCAHGSQLNQVWTNLI"
BASE COUNT          611 a          919 c         1020 g          547 t
ORIGIN      
        1 ttacgtgtca tacacacgag ggtatcggag cgcgaagtca attggaaggt ctgaatcttc
       61 cagcaggttg agtcgtgccg tatgcgttga gcgtacgggc agaaaacgga gcttcatgtc
      121 atcataatgt tgcgcacttg atcgctggta ggcattctct atagccagcg cacaaagcca
      181 agaaggcacc aggagaccta cccgaacgga caggttgccg gggatgctag aatttttgta
      241 gagcgtctcc gcaggctatt cacacgtttt gggggcgctg agtggtcctc gattgtggca
      301 tccaaggagc cgattgcatg gccaaaccgg tgatactcgc tgtggacgac gacccgcagg
      361 tgctgcgggc tgtggagcgg gatcttaaac gcaagtacgc ccgcgagtac agggtgctga
      421 gggcggattc tggagcgtcg gccctcgacg ccctcgacag gctcaaggtc cgcggcgacc
      481 ccgtcgccct gttcctggtt gaccagcgga tgccgaagat gaccggcgtc gagttcctgg
      541 agaaagccat agaggtcttc cccgaggcga agcgcgatct cctgactgca tacgcagaca
      601 ccgacgcggc gatccgggcc ataaacgagg ttggcctcga ctactacctc cagaaaccgt
      661 gggacccgcc tgaggagaat ctctacccca tccttgacga ccagctcgaa gaatggcact
      721 cgggcttcaa cccgcctttc gagggcatca gggtcgtcgg ggatcgctgg tcacccgact
      781 cgcacaggct cagagatttc ctggcccgca acagggtccc ctacaagtgg ctggacgtgg
      841 agggctccga ggaggcgcgt cagatcgtcg ctcgactgga cagcggctcg ccgaagctgc
      901 ctgccgtcgt cttccaggat ggaacgcacc ttgaggcgcc cgagaacagg gaagtcgccg
      961 agagaatcgg gctcaagacc agggcagaga ggccttttta cgacctgatc atcatcggcg
     1021 gcggaccatc ggggttggcg gcagcagtct acggtgcctc cgaaggtctg aaaacggttc
     1081 tcatagagcg tgaagccccg ggtggacagg ccggtaccag cagcttcata gagaactatc
     1141 tcggttttcc ccagggattg aaaggcagtg agttggcccg cagggcggtg gatcaagcca
     1201 aaaaattcga ggtcgagatc ctcacgcccc aggaggtcgc ggatgtgagg gtggaggacc
     1261 cttaccgaat cgtcaggctc ggcgacggca cggagctgtc ctgtcacgcc atgatcatcg
     1321 cgaccggcgt ctcctaccgc aaactgaagg cgccgggggt agatcgcctg gcaggggcag
     1381 gggtgtacta cggcgcgtcg ctcacagagg cgcaatcatg caagaacgag gacgtgtaca
     1441 tcgtcggcgg ggccaactcg gcaggacagg ccgcgatgta cttctccgag cacgcccgca
     1501 aggtgacaat actgtatcgc ggcgacagcc tgaggaagag tatgtccgag tacctcgtga
     1561 aacaggttga gggcaaggat aacatcgagg tgcgcttgaa cagtgaggtg atcgaggtcg
     1621 agggcgaggg ccgcctggag aggatagtcg taagagactc agcctccggc gcgtcggaaa
     1681 ccgttcccgc ccaatcgctc ttcatcttca tcggggctgc gccgcacacc gactggctcg
     1741 gcgaccgcgt cgtgcgtgac gagcgtggct tcatactctc gggttctgac ctgtcgagga
     1801 acgcggaacg ccccagaggc tggaggctgg agcgagatcc cttcctcctg gagaccagcg
     1861 tcccaggcat cttcgtcgct ggagacgtgc gtcacggctc ggtaaagcga tgcgcctccg
     1921 ccgtcggcga gggctccatc gcggtccagt tcgtccacca gtaccttaag gaggtctgag
     1981 gtgcagggcg cggcggtgat cgaaactctc cgcaaggtgc cgctcttctc ggagttgccg
     2041 gatgacggac tcgcctggat ctccgagcgg ggcgaagagg tcaggctgga tgcagggacc
     2101 acgatctcag cccagggcga cccgcccgac ggtttgtacg tcgtactcga aggggagacc
     2161 gagtggacgc gcgaggtcgc tgggcaggag gtcattgtgg cgaaactagg cgaggcttcg
     2221 accttcgcag ggctcttcct cgtgctcgac gcaccgtatc cgactacagg ccgcgccctc
     2281 actcctttac gcctcttcag gttggacgca cctttgttct gggagatgct ccgtacgttc
     2341 ccggaggtct tgcgaggcat cctcgccaat tgcttcgagc gccccgtccc gcgcgatccg
     2401 gtcacccaac agcacgccag cctcatctcc ttaggcacga tggccgctgg cctggcccac
     2461 gagctcaaca acccggccgc ggccgtcacc cggagcgcgc aggaggcccg cgcggcattc
     2521 cgggagtcga tttcgcgtgc catcaagctc ggcgagctgg gtctgagccc tgccgaaagg
     2581 gcgttcgtcg ccggccttcc ggacgaggtt gccagccggg cggagaccat gcctgccctc
     2641 gattcgctgg agcagggcga cagggaggac gaggtcgcgc tctggctgga ggaccgcggt
     2701 atcgatgagg cgtgggaact ctgctccgtc ttcgtaggcg cgggcctcga caccgattgg
     2761 cttgaggaac tcgcggatca tctccccgat gagagagcgc tgggagacgt gctggcctgg
     2821 cttgcctcgg tcataaatgg ggacgcgctg ctgcgcgaga taaagcaggc ctcggcccgc
     2881 gtctccgagc ttgtgggtgc ggtgaagtcc tactctcaca tggacaaggc atcccggaaa
     2941 gaggcggacg tccagaaggg actggataac accctcgtca tgctcggtca caagctcaaa
     3001 aagggcaacg tcagggtgac gcgcgagtac gaagaggatc tgccgaccgt ctgcgcacac
     3061 ggaagccagc tcaaccaggt gtggaccaac ctcatag
//
LOCUS       DATAIR010000283         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_18305, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000283 DATAIR010000000
VERSION     DATAIR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18305"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VHM69_06960"
     CDS             complement(<1..1575)
                     /locus_tag="VHM69_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX2740170.1"
                     /translation="MSKDVAERMRIGNFEEHFDRVSEADWIVEAIIEKLEPKRELMQR
                     VEDTAKVDAIISTNTSGIPLHQISEERSEGFKKRFVGTHFFNPPRYLKLMEIIPTEDT
                     GPEVVEAVRNFGERVLGKGGVIAKDTPNFIGNRLGSFAGMQSARYAFENGYGIEEIDA
                     ITGPLIGHPKTATFRLNDQVGLDIAVGVAENLYEAVPEDESREDLKPPEKLKEMQERD
                     LLGNKTGAGFYKRDKREGKTVFDVLDLETFEYRPAENPEIPIAKDAQQQGDLASKLRF
                     LIEKADEDRYARYIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFAHQTGPFRTWD
                     LLGVRETVEKMESMNIDVGDWVKEMLQAGNESFYKSEEGTELQFSPVSKEYEPVREDP
                     MYISLDRLRDEGKEIARNDSASLLDLGDGVLCLEFHSKGNSIDGQTVEMGNRALEELE
                     RDDVVGLVIGNEGRNFSVGANLGEIAHAVQNGNLDQVGESVEALQDLVMAFRFAPKPV
                     VAAPRGQTLGGGLEVCL"
BASE COUNT          249 a          515 c          467 g          358 t
ORIGIN      
        1 caggcagact tcgagtccgc caccgagagt ctgtccgcgc ggcgccgcga cgacgggttt
       61 cggggcgaag cggaaggcca tcacgaggtc ctgcaaggct tcgacacttt caccgacctg
      121 atccaggttc ccgttctgga ctgcgtgggc gatctcgccg agattcgcac ctactgagaa
      181 gttgcggccc tcgtttccga taacgagccc gaccacgtcg tcgcgctcca gctcctcgag
      241 cgccctgttg cccatctcga ctgtctggcc gtcgatggag ttgcctttgg agtggaactc
      301 cagacagagc accccgtctc caagatctaa caggctcgcc gagtcgttgc gggcgatctc
      361 cttgccctcg tcgcgcaatc tatccagcga gatgtacatc gggtcctcgc ggacgggctc
      421 gtactccttg ctgaccgggc tgaactggag ctccgtgcct tcctcggatt tgtagaagct
      481 ctcgttgccg gcctggagca tctcttttac ccaatcgccg acatctatgt tcatggattc
      541 catcttctcg acggtctctc gtacaccgag caggtcccac gtcctgaaag ggccggtctg
      601 gtgggcgaag ccccactcca tcgcgtggtc cacgtcctcg agggtgtccg agatctccgg
      661 tacccgccgc gacgcgtacg ccatgtaagg cagcaacgta tccctgatgt agcgcgcgta
      721 cctgtcctcg tcggccttct ctatcaaaaa acgaagcttc gaagcaaggt cgccctgctg
      781 ttgcgcatct tttgcgatgg ggatctcggg gttctcggca ggacggtact cgaaggtctc
      841 caggtccagc acgtcgaaga cggtcttgcc ttcgcgtttg tccctcttgt agaagccggc
      901 gcccgtcttg ttgccgagca ggtccctctc ctgcatctcc tttagcttct cgggcggctt
      961 gagatcctcg cgcgactcgt cctcggggac ggcctcgtag aggttctcgg cgacgccgac
     1021 ggcgatgtcc agcccgactt gatcgttgag cctgaacgtg gccgtcttcg ggtggccgat
     1081 gagcggcccg gttatggcgt cgatctcctc gatcccgtat ccgttttcga aggcgtagcg
     1141 cgccgactgc atccctgcga agctccccag gcggttcccg atgaagttcg gggtgtcctt
     1201 ggcgatgacg ccgcccttgc cgagcacgcg ctcgccgaag ttgcggacgg cctcgacgac
     1261 ctcgggaccc gtatcttccg taggtatgat ctccatgagc ttcaagtagc gcggcgggtt
     1321 gaagaagtgc gtccccacga accgcttctt gaagccctcg gaccgctcct cggagatctg
     1381 gtgcagcggt atgccagagg tgttggtcga gatgatggcg tccactttgg ccgtatcctc
     1441 gacacgctgc atgagctctc ttttaggttc gagtttctcg atgatggcct cgacgatcca
     1501 gtcggcctcg ctaacgcggt cgaagtgctc ctcgaagttc ccgatcctca tcctctcggc
     1561 gacgtcctta ctcatgagcg cggcgggcc
//
LOCUS       DATAIR010000284         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110663, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000284 DATAIR010000000
VERSION     DATAIR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110663"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VHM69_06965"
     CDS             complement(<1..685)
                     /locus_tag="VHM69_06965"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740171.1"
                     /translation="MGERRYRQVHLDFHTSAGCEGVGADFDPEAFARTVKIGHVDSMT
                     VFAKCHHGFSYYPTEIGTAHPNLVVPDLMGAQIEALHGIGVRAPIYVSIMWDDLAGEK
                     ESGWIIARKDGSLMIRPPLSNESPLTGGWGWTTLDVSTGYGDYLVAQTEEICDRYDVD
                     GFFFDICFPVPNYSPWGQARMRDAGVRLEDEKAVLRFAEEKLQRFLQRMSNAVRARVP
                     DATIFYNGTV"
     gene            complement(690..1979)
                     /locus_tag="VHM69_06970"
     CDS             complement(690..1979)
                     /locus_tag="VHM69_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX2740172.1"
                     /translation="MAQEMTRWATADTEWFTRDRFGMFIHWGIYALPARHEWVKQREK
                     IRDEDYQRYFDHFYPDLYDPEKWAREAKNAGMRYLVVTTKHHDGFCLWDSDLTDYKVT
                     NTPWGEDLIAPMVEAFRAEGMKVGFYHSLLDWHHPEFPVDGLHSRRDDKEYIEANSDR
                     DISRYREYLHGQTRELLTRFGKIDHMWFDFSYPNRVWGGKGKEDWGSEELMAMVRELQ
                     PGIIVNDRLEIGGDLKTPEQYQPRSWPEVDGEPVLWEACQTLNGSWGYDRDNLDWKPT
                     DMLVRMLVDTVSKGGNMILNVGPNARGEFEPRAVERLRGVGEWMRLHEISVRGCTASD
                     LTPPPDCRYTRNDDRLYLHLFAWPFKHVHLEGLADKVEYAQLLNDASEIKMQVIDPDQ
                     QAQNTTMGGLPPGTLTLELPVQRPPVEVPVVELFLKG"
     gene            complement(2011..>2346)
                     /locus_tag="VHM69_06975"
     CDS             complement(2011..>2346)
                     /locus_tag="VHM69_06975"
                     /inference="COORDINATES: protein motif:HMM:NF028067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2740173.1"
                     /translation="GIFGSRPWEISGEGPSSAEGGAFKEAAIAWTAEDFRFTSREETL
                     YAYQMRPPEDGRSIIRALASGKTPRVVGVRLLGYGSLPFEQTAEGLRITLPQFTPSDG
                     PHGFAIEQG"
BASE COUNT          387 a          758 c          721 g          480 t
ORIGIN      
        1 tgaccgtgcc gttgtagaag atggtggcgt ccgggaccct ggcgcggacg gcgttgctca
       61 tccgctgcag gaaacgttgc agcttctctt cagcaaaacg gagtacagcc ttctcgtctt
      121 ccaggcggac gcctgcgtca cgcatcctag cctgtcccca cggagagtag ttcgggacgg
      181 ggaaacagat gtcgaagaaa aacccgtcca cgtcgtagcg gtcacagatc tcctcggtct
      241 gcgccacgag gtagtcgccg tatccggtgg agacgtcgag ggtcgtccat ccccatcccc
      301 ctgtcagggg cgactcgttc gagaggggag gccggatcat gaggctcccg tccttgcggg
      361 ctatgatcca gcccgactcc ttctcgccgg cgaggtcgtc ccacatgatg ctcacgtaga
      421 tcggagcccg caccccgatc ccgtgaagcg cctctatctg cgcgcccatg agatccggca
      481 ccacgaggtt cggatgcgcc gttccaatct ccgtcgggta gtacgagaag ccgtggtggc
      541 acttggcgaa gaccgtcatc gagtcgacgt ggccgatctt taccgtcctg gcgaaggctt
      601 ccggatcgaa gtcagcaccg actccctcgc agcctgccga ggtgtggaag tcgaggtgga
      661 cctgccggta tcgtcgctcg cccatccgcc tatcccttca ggaatagctc gacgacaggc
      721 acctcgacag gcggcctctg caccggcagc tcgagggtca gagtacccgg cgggaggcct
      781 cccatggtag tgttctgggc ctgctggtca gggtctatga cctgcatctt tatctccgag
      841 gcgtcgttga ggagttgtgc gtactcgacc ttgtccgcca gcccttcgag gtggacgtgt
      901 ttgaatggcc acgcgaaaag gtgcaggtag agtctgtcat cgttccgggt atagcggcag
      961 tcgggcggcg gggtgagatc gctcgccgtg cagccccgca cggagatctc gtgcaaccgc
     1021 atccactccc cgacaccgcg cagtctttcc acagcccgag gctcgaactc gccgcgggcg
     1081 ttcggaccta cgttgaggat catattaccg cccttggaga ctgtatccac gagcatcctg
     1141 acaagcatgt ccgtaggctt ccagtccagg ttgtcccggt cgtagcccca tgatccgttg
     1201 agagtctggc aggcttccca gagcaccggt tcgccgtcca cctcgggcca gcttcgcggc
     1261 tggtactgct ccggagtctt gaggtcgccc ccgatctcca acctgtcgtt gacgattatg
     1321 ccgggctgaa gctcgcggac catcgccatc agttcctccg agccccagtc ctctttgccc
     1381 ttgccgcccc agacccggtt cgggtacgaa aagtcgaacc acatgtggtc tatcttcccg
     1441 aacctggtca ggagctcgcg ggtctggccg tgcagatact cgcggtagcg ggagatgtcg
     1501 cggtctgagt ttgcctcgat gtactccttg tcgtcacgcc tggagtggag gccgtctacg
     1561 gggaactcgg gatggtgcca gtcgaggagg gaatggtaga agccgacctt catgccttcg
     1621 gcgcgaaacg cctcgaccat cggggcgatg agatcctcac cccacggagt attggtgacc
     1681 ttgtagtcgg tgaggtcaga gtcccagagg cagaagccgt cgtggtgctt ggtggtgacg
     1741 accaagtacc tcatccctgc attcttcgcc tccctcgccc acttttcggg atcgtagagg
     1801 tcggggtaga agtggtcgaa gtaccgctgg tagtcctcgt cccggatctt ctcgcgctgc
     1861 ttgacccact cgtggcgggc cgggagggcg tagatccccc agtggataaa catgccgaag
     1921 cggtcacgcg tgaaccattc cgtgtcggcg gtcgcccacc gcgtcatctc ctgcgccatt
     1981 tcctccccat tcctttcgct tttcctgcca tcacccttgc tcgatggcga agccgtgcgg
     2041 cccatcggaa ggcgtgaatt gcgggagcgt tatccgcagc ccctcggccg tctgctcgaa
     2101 tggtaagctc ccgtacccga gcaggcggac cccgacgacc cgcggcgtct tgcccgaggc
     2161 cagcgctcgg atgatggacc gtccgtcttc gggtgggcgc atctggtagg cgtagagcgt
     2221 ctcctccctg ctcgtaaagc ggaagtcctc agccgtccac gcgatcgcgg cctccttgaa
     2281 ggctccccct tctgcgctgg acggtccttc gccggagatc tcccaggggc ggctgccgaa
     2341 gatgcc
//
LOCUS       DATAIR010000285         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_192777, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000285 DATAIR010000000
VERSION     DATAIR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192777"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHM69_06980"
     CDS             complement(<1..536)
                     /locus_tag="VHM69_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740174.1"
                     /translation="MQAAAALWDGGFQAVELTYFAVRGNRWLIPILKEMDLLVVVGSI
                     TRSAQVREPDVIGADLFATSVIAPDVLLACEEADVPCILGALSPTEIWRAHEMGPDFV
                     KVTDPEALGGSHYAATLRESLPTLHLVGGNMPLHGYLSYLEEGVEVLEFGGALLLPQP
                     TLRDEWVELSRRASEIVAA"
     gene            complement(898..1611)
                     /locus_tag="VHM69_06985"
     CDS             complement(898..1611)
                     /locus_tag="VHM69_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2740175.1"
                     /translation="MVSRILLVEDDPAVRDIIKIALEREGMTVEDAGDGESALRLFRS
                     FRTPDLVVLDIMLPGIDGITVCQEIRRMSNVPIVVLTARDDERDVVVGLEVGADDYVT
                     KPVSPAELVSRVRAHLRRRRMDARDSDQRLEFPGLVIDLLRRQVWVGEVNVYLTATEF
                     EILTLLAAHPGRVFSRHQIMEQLWDGAYFGEAHSTVVHIQHIREKIEPDPRKPHYILT
                     VRSTGYKFVEDVVEPPYDS"
     gene            complement(1586..>2716)
                     /locus_tag="VHM69_06990"
     CDS             complement(1586..>2716)
                     /locus_tag="VHM69_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX2740176.1"
                     /translation="RNKPVRDESGNLVAIEGIARDVTRRKLADGEREQFLDRERAAHQ
                     HLEAILDNLGEGVLVIGSGESVLFANPSAQVMLGMTDDSTRAELPDPCEDFSLRDAVD
                     RCIRTGEAIEARARCGGKFLQISLDRLGWLEENEVLVVLQDLSEGHRLEANQQRFLSN
                     AAHQLKTPLTVILGAAELLASGRDEDPALRERLLNHILSEGRRMKRLSDVLLGLARIG
                     WGEREPMIEPLNLGAAASYAAELVEPLAESAGLGITVEGEGTRVYADSEWLREVLLVL
                     LSNSIQHSGRGGFLRLVVRGATVTVEDVGVGISPEDLPHVFERFYRGRDNSEGFGIGL
                     SICKELTERMGGSISIRSREGVGTAIKIALPEVGDGVTDTPG"
BASE COUNT          516 a          884 c          762 g          554 t
ORIGIN      
        1 gccgcgacta tctcagaggc cctccgagag agctcgaccc attcatctcg caatgtcggc
       61 tgcggcagca acagtgcacc cccgaactcg agcacctcga ccccctcttc aaggtacgaa
      121 aggtatccgt gcagtggcat attcccgccc acaaggtgca gcgtcggcaa cgactcgcgc
      181 agcgtagcgg cgtagtgcga ccctccaagc gcttcgggat cggttacctt cacaaaatcc
      241 ggtcccatct cgtgtgcacg ccagatctcg gtcggactca gagcgccaag gatgcaaggc
      301 acgtctgcct cctcacacgc aaggagcaca tcaggggcta tcaccgaggt ggcgaaaaga
      361 tcggcgccga tcacatcagg ctcgcgaact tgcgctgagc gggttatgga ccctacgacg
      421 accaggaggt ccatctcctt tagaatcggg atcagccagc ggttacctcg aacggcgaag
      481 taggtcaact ccacagcctg gaaacctccg tcccacaacg ctgcggccgc ttgcactagg
      541 cgccgttcgt cctgtagagg caagcgagcc atcatgcctg cgtccctgag tgccgttgcc
      601 gaatccatcc tattcaacgt ctgcaggtcc tctcactccg gatgcttgtt gttgcgaccc
      661 ttccggtatc cgatgcttcg gtacaacctc cataccggca ataatcatcg ccaggtttgg
      721 attggcctcg tagtgtattg tgacttgcct gtctaaacct ttagcatatt tcggatcaaa
      781 ctatctttat tcttgctaga taggggtcgc ctgatgatgg tgcgcgacct tggcgaaatg
      841 aaggtcgaaa tcaccttttc aggccgcgct atctcaggtg gacttttcta agtccgatta
      901 tgaatcgtaa ggaggctcga ctacatcttc tacgaacttg tacccggtgc ttcgcacggt
      961 gagtatgtag tggggcttcc tgggatcggg ctcgatcttc tcgcggatat gctgaatgtg
     1021 cacgaccgta gaatgggcct cgccaaagta ggctccgtcc cagagctgct ccatgatctg
     1081 gtggcggctg aagacccgtc ccgggtgcgc cgcgagtaag gtgaggatct cgaactccgt
     1141 ggcggtaagg tagacattta cctcacccac ccatacctgc cggcgcaaga gatcgatgac
     1201 cagcccgggg aattccagtc tctggtcaga gtccctggcg tccatgcgcc tccggcgcag
     1261 gtgggcccgg acgcggctca ccagctccgc ggggctgacc ggtttggtga cgtagtcgtc
     1321 ggcgccgacc tcgagcccaa cgacgacgtc tctctcgtcg tcgcgcgccg tgagcacgac
     1381 gatcggtacg ttgctcatcc tcctgatctc ctggcagacg gtgatgccgt cgatccccgg
     1441 caacattatg tcgagcacga cgaggtcggg agttctaaac gaacggaaaa gtctcaacgc
     1501 cgactcgccg tccccggcgt cctccaccgt cattccctcg cgttcgaggg caatcttgat
     1561 gatgtccctt accgcgggat cgtcttcaac caggagtatc cgtgacacca tcacctacct
     1621 cgggcaaagc gatcttgatc gccgtgccga caccttcccg cgagcggatg gagatactgc
     1681 ctcccatgcg ctcggtgagc tctttgcaga ttgaaagccc gatgccgaat ccctcggagt
     1741 tgtccctccc ccgatagaac cgttcgaaga cgtggggaag atcttctggg cttatgccga
     1801 cgcccacgtc ttccacggtg acggtggctc ctctcactac tagccgaagg aacccgcccc
     1861 gcccggagtg ttggatagag ttgctgagca gtaccagcag tacctcccgc aaccactcgg
     1921 agtccgcgta cacgcgcgta ccctcacctt cgactgttat gccgagtcca gcgctctccg
     1981 cgagcggctc caccagctcg gcggcatagc tggcggcggc gccgaggttc agcggctcga
     2041 tcatgggctc cctctctccc caccctatgc gcgccaggcc gagcaagacg tccgacagac
     2101 gcttcatccg ccgaccttcg gaaaggatgt ggttcagcag gcgctctcgc aaggctggat
     2161 cttcgtccct accggaggcc agcagctctg cggcaccgag gatgacagtg agcggcgtct
     2221 tgagctgatg ggcggcgttg gacaagaaac gctgttggtt ggcctccagc cgatgccctt
     2281 ccgagaggtc ctgcaaaacc acgagcacct cgttctcctc cagccagccc agccgatcca
     2341 ggctgatctg aaggaacttc ccaccgcaac gcgcccgggc ttctatcgcc tccccggtcc
     2401 ggatgcacct atccaccgcg tcccgcaggc tgaaatcttc gcaagggtcc ggtaattccg
     2461 cacgtgtaga gtcatccgtc atgcccaaca taacctgggc ggaagggttg gcaaagagga
     2521 ccgattcccc agacccgata acgagtacgc cctcgccgag gttgtccagt atggcctcga
     2581 ggtgctggtg agccgctcgt tcccgatcca ggaactgctc gcgctccccg tcggccagct
     2641 ttcttcgggt cacgtcgcgg gcgatgccct cgatggccac caggttgcct gattcgtcgc
     2701 gtaccggctt gttgcg
//
LOCUS       DATAIR010000286         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_18411, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000286 DATAIR010000000
VERSION     DATAIR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18411"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="malQ"
                     /locus_tag="VHM69_06995"
     CDS             <1..874
                     /gene="malQ"
                     /locus_tag="VHM69_06995"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2740177.1"
                     /translation="GAANAAGIEVIGDVPIFVLHDSADVWANQHHFFLDERGEPTVVA
                     GVPPDYFSEAGQLWGNPIYRWERMRENGYSWWIARLRMALTLCDVVRVDHFRGFQAYW
                     EVPAGEETAANGRWVEGPGAELFAALRHALGELPLVAEDLGDITPEVVALREEIGLPG
                     MKVLQFAFSDPGSPFLPHNHAGVDWVVYTGTHDNDTTAGWWNSASLEERTFARHYVGK
                     EYVGVWDFVRLAYCSVAKRTVVPMQDVLGLGSEARMNTPGTVEGNWAWRMSGTELTPE
                     LAARLKRLAEIYGR"
     gene            1397..2086
                     /locus_tag="VHM69_07000"
     CDS             1397..2086
                     /locus_tag="VHM69_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740178.1"
                     /translation="MQAIDEEKSVLRWGGLAGILGGIVFILVFVVVIAFVGPEPVQPE
                     GKVMRFPDIRAARTVEDGLYLVVLILWVTHFLALYRALRGTSLAPALFGSVLGIVGLG
                     VLAAGALPHVAQAPISDLYHAPGATTEDRAPLVLVWQATEGIFDAMFGAGLLLVPLGF
                     IALGVAMLRVPAFGRGFGGVSVALGVVGIVAATVLLVDPLSPSAFVGVLALIVFHLVL
                     GWKVYSLSRSP"
     gene            2152..3129
                     /locus_tag="VHM69_07005"
     CDS             2152..3129
                     /locus_tag="VHM69_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2740179.1"
                     /translation="MKAIVQDKYGSADVLELRDVEKPEPGDDEVLIRVHAAGIDPGVW
                     HLMTGLPYLVRVMGFGLRKPKIRIRGSDVAGTVEAAGRNVTQFKEGDPVYGTCDGSFA
                     EYACARAERFAPKPANLSFEQAAAVPISSMTALNGLRDAGKLQPGQNVLIIGAAGGVG
                     TYAVQLARAFGSMVTGVCSTSKAELVKSIGADEVIDYTREDFTDGTRQFDLILDTAGR
                     RPLSHLRRALTPQGTLVIVGGEGGDRWLGGFQRQIFAPVRSRFSQQKLLGLISKERQQ
                     DLLTLKDLIEEGKLTPVIDRTYPLSEAAKAIRYLEQGHARGKVVLSISE"
BASE COUNT          861 a         1358 c         1405 g          883 t
ORIGIN      
        1 gggggcggcc aacgctgccg gcatcgaagt gataggcgac gtacccatct tcgtcttgca
       61 tgactccgca gatgtttggg ccaaccagca ccacttcttc ctcgatgagc gcggcgagcc
      121 gaccgtcgtg gccggggtac cgccggacta cttctccgag gcggggcaac tctggggtaa
      181 tcccatctac cgttgggagc ggatgcgcga gaacggctac tcctggtgga tcgcgaggtt
      241 gcgtatggct ctcaccctct gcgacgtggt gcgggtagac cacttccggg gcttccaggc
      301 ttactgggag gtccccgcag gcgaggagac agccgcgaac ggccggtggg tagagggtcc
      361 cggggcggaa ctcttcgccg ccctgcgcca tgccctgggg gagctgccgc tcgtcgccga
      421 agaccttggc gacattaccc ctgaggtcgt cgcgctgcgc gaggagatcg ggttgcctgg
      481 catgaaagtc ctgcagttcg ccttctcgga cccgggcagc ccgtttctgc cgcacaacca
      541 tgccggagtc gactgggtcg tctacaccgg cacccacgac aacgacacta ccgccggctg
      601 gtggaactcg gcgagcctcg aagagaggac cttcgcccgg cactacgtcg gcaaggagta
      661 cgtcggcgtc tgggacttcg tgcgcctcgc gtactgctcg gtggcgaaaa ggaccgtagt
      721 gccgatgcag gacgtgcttg gcctgggctc cgaggcccgg atgaacactc ccggcacggt
      781 ggagggaaac tgggcgtggc gaatgagcgg cacggagctt acccccgaac ttgccgctcg
      841 cctgaagcgg ttggccgaga tctacgggcg ctagagggac gtccggcacc gcgactcgtt
      901 atttcctgag agccgacgag cggactcgta ccgctgacct aatctcgtta cgggtgatag
      961 ttcatgagtt gccgggggct caggtagact gcaacgtgct gttgatctct cgccgttgct
     1021 gctgagcttc cgagggtgcg gcaatagttc ctcaccctct gcgcttcact cacgggccac
     1081 gtcgccccac atggagatga ggtaaacgca ccttcaggtc ccacgtttct taaggtgttg
     1141 aagatagtag gcaatgtccg tgaggtcttg caggggcttc ttctccgaga gtcgcccgct
     1201 ctcgtagttc tcgagaccca acatctttcg atctccggac cgatggcaaa caaccctctc
     1261 cagagtaagg tccttgaata ggtgacaggc ggcaagaagg gagcagggag atgaacaatt
     1321 cgttcagcgt caacctactt ggtttaaaga agtttgggtc taagggatag tcgccaaaca
     1381 aacctggggg aaacccatgc aagcaataga tgaggaaaag agcgtcctac gatggggtgg
     1441 tctggcaggt atcctgggcg gcatcgtctt cattctcgtc ttcgttgtgg tgatcgcgtt
     1501 cgtgggaccg gagcctgttc aacctgaggg gaaggtcatg agattccccg acataagagc
     1561 agcgcgcacg gtagaggatg gactgtacct ggtggtcctc atcttgtggg tcacccactt
     1621 cctggccttg tatcgtgctc tgcgggggac aagcctcgcg cccgctctct tcgggagcgt
     1681 cctgggcatt gtgggactcg gcgtgctcgc ggcaggagct ctcccgcatg tagcccaagc
     1741 cccgatctcc gacctctatc atgctcccgg ggcgaccacc gaggatcggg cgccactcgt
     1801 cctcgtgtgg caggcgactg agggcatatt cgacgcgatg ttcggcgcag gactcctcct
     1861 ggtgccttta ggtttcattg ccctcggcgt ggccatgctc agagtcccgg ccttcggcag
     1921 gggctttggc ggggtgagcg tggcgctcgg ggtggttggg atcgtagcag caactgtcct
     1981 tctcgtcgat cccctctctc caagcgcctt cgtcggagtt ctcgcactga tcgtcttcca
     2041 tctagtcctt ggatggaagg tgtacagtct gtcgaggagc ccgtagtgag tcttgaagtt
     2101 aggagagaag ccgggcgctg aagtcaagag aggaaggaag taacggcagc catgaaagcc
     2161 atcgttcaag acaagtacgg ctcggccgat gtgctcgagc ttcgcgacgt cgagaaaccg
     2221 gagccagggg acgacgaagt gctcatccgc gtgcatgctg cgggcatcga cccgggcgtc
     2281 tggcatctga tgacgggcct tccctatctg gtccgcgtca tgggttttgg cttacgcaag
     2341 cccaagatcc gcatacgtgg cagtgacgtc gcagggaccg tcgaggcagc ggggagaaac
     2401 gtcacccagt tcaaggaggg tgatccggtt tacgggacgt gcgatggctc cttcgcggag
     2461 tacgcatgcg ccagggcgga gcgcttcgca ccgaaaccgg cgaacctaag cttcgagcag
     2521 gccgcagccg tccctatctc cagcatgacc gccctcaacg gcctgcgcga cgcgggcaag
     2581 ctccagccag ggcagaacgt cctcatcatc ggtgccgcgg gcggcgttgg gacctacgcg
     2641 gtgcagcttg ccagggcgtt cggatcgatg gtaacaggcg tgtgcagcac gtccaaagcg
     2701 gagctagtga agtccatcgg cgccgacgag gtcatcgact acacccgaga ggacttcacc
     2761 gacggcacgc gccagttcga cctgatcctc gacaccgcgg gacggcggcc attgtcgcac
     2821 ctgcggcgag ccctcacgcc gcaaggcacc ctcgtcatcg tcggcggtga aggcggagac
     2881 cgctggctcg gtggcttcca acgccagatc ttcgcccctg tgcgctcgcg cttttcgcag
     2941 cagaagctgc tcggcctcat ctccaaggag cgccagcagg acctcttgac actcaaagac
     3001 ctgatagagg aaggcaagct cacgccggtc atcgacagga cgtacccgct gagcgaggcc
     3061 gctaaggcca ttcgctatct cgagcaaggc cacgcgcgtg ggaaggtcgt gctcagcatc
     3121 tcagaataga ggggcctcat accttgcgcg gataggtcat gggctgcgag gcgatcggct
     3181 ctcccaacgg tagcggctcc atgcttcgtg ggtcgacctc ctcacgatga tgaccacctc
     3241 agagaaggcc acccaagatc gatcaccctc ccaccacgct ccgtgcacca cacgagcttt
     3301 ccctggtcta gcttcgcttg ccactaactc gtcctctcca cccatcgact ggtggcccag
     3361 aacggtgccg actcgccctt catgaagagc tctcatctat attgagagag ccgacgagcg
     3421 gactcgaacc gcttgctcat tacgagtgag cggtcagcgg tcgcggagcg ttgcacgggc
     3481 ttgcgaatcg cgcataaata ggcgattcat tgttctctcg attgcccggt cttgcagcgt
     3541 gttgcatccg ggtcagggtc aaactagaga caagagagta tagattacgc gtcgccggtt
     3601 cctttgcaaa ccaggtgcgg caggcgctag agtgtccgcc gcacctacga ggcgaagtcc
     3661 ctgggagaga cttccatctc tgtacaggcc tctccgtccc attcaccagg gcggataccc
     3721 atgcagggct aacacatgta gcgttgacta caatgcgtag gactgggact tagggtcttt
     3781 tctggctccg taggaccgcg gctatgcagg atcctggata tggacttctg agaatccacc
     3841 atcctgttgt aaaagtcggc attgagcagg atgcgaccac aaatcgtgct cgaaacgccc
     3901 caaaaccaac gcatttggtg cccaatctgg ggcgaaagcg ggtaccaaga aagtgtttca
     3961 acaggctgca accttccacg ctgtgagtat cctggagaat ggctgtgggc ggcgactcgg
     4021 accggcctga ccctctttcc acgtgggccc taaagtgcct tcggagtgac tcgtcggccg
     4081 gacgccttgg tgtcgcccaa cagtcaacgc tagcgtgagc acttcgggcg ccccaaaaga
     4141 atattcccgg ctctttttcg gcgctcccgc gagcgtccgc ccgattttgc ttctcgatca
     4201 tcattatttt cctcgacgag ccttggaggt tgcgctgata ggctcgcagc gctttatgtt
     4261 tgccgattct ccgaacgatc agatgatcgg cctctcgatc gaggatgctg gggacacatg
     4321 cgacgtcaaa cctgagcgct aggcactggc acggaagcta gccacgcggc aaaggacgga
     4381 ccgaccaggc gagcccgaga tccgggcagt aggatgccgt gtcgcaccgc atcgcccacc
     4441 ggcgcagcga cgattgagac ggagtccccg acgtggaccg catacctggt cgagaggtcc
     4501 gcaagct
//
LOCUS       DATAIR010000287         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_18467, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000287 DATAIR010000000
VERSION     DATAIR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18467"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VHM69_07010"
     CDS             complement(<1..1439)
                     /locus_tag="VHM69_07010"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2740180.1"
                     /translation="MSERTKSRSLRAPRRVRAYLAANSERPALLLAASEEAAERYARE
                     AAVFTDGLVVHLPSRGVPYGDVFDPAVTRVGRRQRALRSLRSARVVVAGPLAFMERTP
                     LYEPMDLASGTEIELDATLERLVVLGYERVDRVSRPGEFAVRGGIVDLFPSTRRSPVR
                     VEWWGDEIESVRAVSLATQRVVRGLEAVTVYAAREGDLAGLAATDEELPEEARRGVRV
                     PGLDRLLLGLNPVSPRTLLPERVEVWREEPREDIPEDVDGLVPDLYEGEMPEPDVEFA
                     LGEEGETVSAPPVAAFADTLREAARRLDSLVESGLAVFVACSSGGEARRTVYAFGEIH
                     RTMREAERVDASLPPGLYALPGEVEEGFTYPEGGVAVVRRDSLLGRRREARRRTGGRA
                     LASFADLKVGDLVVHAMQGIGRFEGLVAQEVLGATRDYMQVSYRGGDTLFVPYEQMEL
                     LHKYVGGDGARLDKLGGATWAQVTDRVRKR"
     gene            1536..1946
                     /locus_tag="VHM69_07015"
     CDS             1536..1946
                     /locus_tag="VHM69_07015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2740181.1"
                     /translation="MSILEDLNKRGIGTMPGLIGLEILEAEAGRVASRLELRQELMAP
                     NGYLHAATVIALADTSCGYGTFVNLPEGAHSFTTIELKSNFVGTKREGAINCEANLVH
                     GGRTTQVWDARVTDDENGKPIALFRCTQMILYPR"
     gene            complement(1951..2823)
                     /locus_tag="VHM69_07020"
     CDS             complement(1951..2823)
                     /locus_tag="VHM69_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2740182.1"
                     /translation="MKPGDFAGLILLGALWGGSFLFIRVAVPALGPFLLVDLRVGLAA
                     AALFLYALAASRVPKIRYRWKSFLALGFLNAAVPFSLISAAEIHLTASLAAIMNSTTV
                     MFTAIVAAVWMGEALTARKLVGIVLGIFGVAVLVGWDPISVNGMVLLSVAAMLGASLA
                     YALGATYAKQAFSGIPPLGMAVGQLTAATALLLPLSAASVPTEVPSLAVALCVLGLAL
                     LSTAVAYLIYFRLIENVGPTSTVTVTLLVPVFGLLFGVLLLDEPFGFGTLAGLGIILS
                     SVVLITGFAPGRSR"
     gene            complement(3039..3560)
                     /locus_tag="VHM69_07025"
     CDS             complement(3039..3560)
                     /locus_tag="VHM69_07025"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2740183.1"
                     /translation="MKAYVDEIDSPAGPLAFAVDEEGALIRLHFMESDQGSEMEEDLE
                     DEGYAVEADETRTASVREELSEYAHGRRKTFDASLAFVGSEWQKAVWMELTRIPFGET
                     RSYGEIADALGRPGAARAVGRANASNVLPLVVPCHRVIAADGTLGGFNGGLHLKERLL
                     EHESRVLAGHGEQ"
     gene            complement(3557..5044)
                     /locus_tag="VHM69_07030"
     CDS             complement(3557..5044)
                     /locus_tag="VHM69_07030"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX2740184.1"
                     /translation="MLKDFEHRYRAVRGRDVRFDGWFYVAVTSTGIYCRPSCPAVTPK
                     RSNVRFYPTAAAAQYAGFRACKRCRPDAVPGSPEWDVRADVVARAMRLIADGVVDREG
                     VAGLARRLSYTERHLHRLLSAELGAGPLALARARRAHTARLLIETTDLPITEIAFAAG
                     FTSIRQFNDTIREVFAVTPRELRRARRHSGESARGEISLRLPFRTPFDAKGLLHFLGG
                     RAVPGVETFHGETYMRTLRLPHGAGTVALSDGGDHVHCVLRLENLRDLGTAVQRCRRL
                     LDLDADPVAVADVLRADHVIGNLVRRSPGRRVPGSVDGAELAVRAVLGQQVSVAGAST
                     LAGRLVAGCGDPVPESLAGNELTHLFPGPDTVAETAPEQFAVPEARRETLRSLAGALA
                     GGEIVLDPGSDREEAERLLLSLRGIGPWTASYIAMRALGDPDAFLPTDLGVRRAVSRL
                     AGDQRSVAALSERWRPWRAYATQYLWASLGDVARTEDTTMREVIT"
BASE COUNT         1003 a         1780 c         1688 g          846 t
ORIGIN      
        1 cgcttgcgga ccctgtcggt gacctgcgcc cacgtcgcgc cgccgagctt gtcgaggcgg
       61 gccccgtcgc cacctacgta cttgtgaagg agttccatct gctcgtacgg aacgaagagc
      121 gtatcgcccc cacggtagct aacctgcatg tagtcgcggg tggcgccgag tacctcctgg
      181 gcgacgagcc cctcgaagcg tccgatgccc tgcatggcgt gtacgacgag atccccgacc
      241 ttcaggtccg cgaatgaagc cagcgcacgg ccgcccgtac ggcgacgagc ctcgcgtctg
      301 cggccgagga gcgagtccct gcggacgacc gcgacaccgc cctcgggata tgtgaacccc
      361 tcctcgacct cccccggcaa cgcgtagagt cccggcggaa gagaggcatc cacccgctcg
      421 gcctcgcgca tggtgcggtg gatctccccg aacgcgtata ccgtccgcct cgcctccccg
      481 cccgaggaac aggcgacgaa gacagcgagc ccgctctcga cgagcgagtc gagcctgcgg
      541 gcggcttccc tcagggtgtc ggcgaacgcc gcgaccggcg gggcggagac ggtctccccc
      601 tcttcaccga gggcgaactc gacgtccggc tcgggcatct caccttcgta gagatcgggg
      661 acgagcccgt ccacgtcttc cggaatgtcc tcccgcggct cctcgcgcca gacttcgacg
      721 cgctcgggca gaagggtgcg cggcgagacc gggttcaggc cgagcaacag gcggtcgagg
      781 ccggggacgc gaactccgcg ccgtgcctcc tccggaagtt cttcatccgt cgccgcgagc
      841 cctgcgaggt cgccttcgcg ggcggcgtag accgttacgg cttccagccc gcgcacgacc
      901 cgctgggtcg ccagggagac ggcgcggacg ctctctatct catcccccca ccactcaacc
      961 cgcacgggcg agcggcgggt gctcgggaag aggtccacga tcccaccacg caccgcgaac
     1021 tcgccggggc gagagactct gtcaacccgc tcgtagccga gtaccacgag ccgctccagc
     1081 gtcgcgtcga gctctatctc cgtcccggag gcgagatcca tcggctcgta aagcggcgtc
     1141 cgttccatga aggccagcgg tccagccaca acgacgcggg ccgaacggag cgaacgcagc
     1201 gccctttgcc gccgcccaac ccgcgtcacg gcagggtcga agacatcacc gtaagggacg
     1261 ccgcgggagg gcaggtgaac cacgagaccg tccgtaaaga cggcggcctc acgagcgtag
     1321 cgctcggccg cctcctcgct cgcggcgagc aggagcgcgg gacgctcgct gttcgcggcc
     1381 aggtaagccc ggaccctgcg cggggccctg agggatctcg acttagttct ttcgctcacc
     1441 ggtagattct accgcctgag cgtggatctc aagcaacgaa acgcgcctct gggataatcc
     1501 cttgagctgg aacatggacc ctggaggcgc gtcaaatgtc tattctcgaa gatctaaaca
     1561 agcgcggtat cggaaccatg cccggtctca ttgggctgga gatattggag gcggaggccg
     1621 gcagggtcgc cagcaggctc gaactgcgcc aggaactaat ggccccaaac ggctacctgc
     1681 acgcggcgac ggtcattgca ctggcagata cctcgtgcgg ctacgggacc ttcgtcaacc
     1741 tgcccgaagg agcacatagt ttcacgacga tagagctcaa gagcaacttc gtcggcacca
     1801 aacgagaagg tgcgatcaat tgcgaagcca acctcgtcca cggtggccgc acgacccagg
     1861 tatgggacgc cagggttaca gacgacgaga atggcaagcc catagccctc ttccgatgta
     1921 cgcagatgat cctctacccg cgctgaacga tcatctactt cttcctggag cgaaccccgt
     1981 gatgaggacc acgctcgaca agatgatccc gagcccggcc agcgtgccga acccgaaagg
     2041 ctcgtccagc agcagaacgc cgaacaggag cccgaagacc ggcacgagca gcgtgaccgt
     2101 gacggtgctc gtcggcccga cgttctcgat gagccggaag tagatcaagt aagctactgc
     2161 cgtcgacagc aatgccagcc caagcacgca gagggccacg gccaacgatg gcacctccgt
     2221 gggaacgctg gcagcagata gaggcaagag gagcgcagta gccgccgtca gttgcccgac
     2281 cgccatcccg agtggtggta tgcccgagaa tgcctgtttg gcgtaggtgg cccccagcgc
     2341 gtaggcgagg gacgcaccga gcatcgccgc cacagacagc agcaccatcc cgttcacgga
     2401 gatcgggtcc caaccaacga gcacagcgac cccgaagatc ccgagtacta tcccgacgag
     2461 cttcctggcc gtcagggctt ctcccatcca gaccgcggcc actatcgcgg tgaacatgac
     2521 ggtggtcgag ttcatgatcg ctgccagcga ggccgtcagg tggatctcgg ccgcggagat
     2581 cagggagaaa ggcacggccg cgttcaggaa ccccagggcg aggaacgact tccaacggta
     2641 ccggatcttg ggcacgcggc tcgctgcaag ggcgtagaga aacaaagccg ccgcggcgag
     2701 tccgacacgc aagtccacga gcaagaacgg tcccaacgcc ggcacggcga cgcggataaa
     2761 caggaacgaa ccgccccaga gcgcgccgag caatatgaga cccgcgaaat cccctggttt
     2821 cacagaatgc tactttgaga tggaaggagc acttttctac gcatcatgcg gcagacgacg
     2881 gggccgcgcc acaagttcac cgccacaatt ggggcagatg ttacccattt cggacgtaca
     2941 cgcggcgcag aatgtgcatt cgtagctgca aatgaaggcg attccgtcat ctttgagtgc
     3001 ggcgttgcag atctcgcacc cggggcgcat ctccaggctt actgttcgcc gtgcccggcg
     3061 agtacccggc tctcgtgttc cagcaaccgc tccttgaggt gcagcccgcc gttgaaaccg
     3121 ccgagcgtgc cgtctgcggc gatcacacgg tggcagggca cgacgagtgg aaggacgttg
     3181 gaggcgttcg ccctgccgac ggccctcgct gcgcctggac gtccgagcgc gtcggcgatc
     3241 tccccgtagg acctggtctc gccgaagggg atgcgagtca actccatcca tacggccttc
     3301 tgccactccg agcccacgaa cgcgagagat gcgtcgaagg tcttgcgcct tccgtgcgcg
     3361 tactcggaca actcttcccg cacgctggcg gtcctggtct cgtccgcttc cactgcgtaa
     3421 ccttcatcct ccaggtcctc ttccatctcc gatccctggt cactctccat gaagtgaagc
     3481 cggatcaggg ctccttcctc gtctaccgcg aatgcgagcg ggcctgcggg agaatctatc
     3541 tcgtcgacgt aagctttcac gttatcacct ctctcatggt cgtatcttcg gtcctggcca
     3601 cgtccccgag gctggcccat agatattgcg tggcgtaggc gcgccatggc ctccaccttt
     3661 cggatagcgc ggcgacgctc cgttggtcgc ccgcgagcct ggagacggcc cgcctgacac
     3721 cgaggtccgt tggaaggaac gcgtcggggt cgccgagcgc gcgcatcgct atatacgaag
     3781 ccgtccaggg tccgatccct cgcagactca acagaagccg ttctgcctcc tcgcgatctg
     3841 agccggggtc gagcacgatc tctcctccgg cgagagcacc cgccagactt cgcagggtct
     3901 cgcgacgggc ctcgggtacg gcgaactgtt ctggagcagt ctcggctacg gtatccggcc
     3961 cagggaagag gtgggtcagt tcgtttccgg cgagcgactc gggtaccgga tccccgcaac
     4021 cggcgacgag acgacccgct aaggtgctcg cgccggccac gctgacctgc tgcccgagca
     4081 ccgcccgaac ggcaagctcg gcgccatcta cgcttcccgg cacgcgtcgg ccgggtgaac
     4141 gacgcacgag gttgccgata acgtgatctg ccctgagtac gtcggcgacg gcgacagggt
     4201 ctgcgtcgag gtcgaggagc cggcggcaac gctgtaccgc ggtaccgagg tcccgcaggt
     4261 tctcgagcct gagcacgcaa tggacatggt cgccgccgtc cgagagggcc accgtcccgg
     4321 cgccatgcgg caggcgcaac gtccgcatgt acgtctcccc gtgaaacgtt tcgacgccag
     4381 gcacggcgcg accaccaagg aaatggagca gacctttcgc gtcaaatggc gtcctgaagg
     4441 gcaatcggag tgagatctcc ccccgcgcgc tctcgccgct gtgtcgtcgc gcccggcgca
     4501 actctctcgg ggtaacggcg aagacctcgc ggatcgtgtc gttgaactgg cggatgctcg
     4561 tgaagcccgc ggcgaacgcg atttcggtaa tgggtaaatc cgtggtctct atgagaagcc
     4621 tggcggtgtg cgcccggcgc gcacgggcga gcgcgagcgg accggccccg agctcggccg
     4681 agagcagacg gtgcaggtga cgctccgtat aggagagcct gcgcgcaagc cccgcgacgc
     4741 cctccctgtc aacgacgccg tcggcgatga gccgcatggc cctggcgacg acgtcggcgc
     4801 ggacgtccca ctcgggcgaa cccggcacgg cgtccggcct gcaccgcttg cacgccctga
     4861 aaccggcgta ctgcgctgca gcggccgtcg gataaaagcg tacgttggaa cgcttcggcg
     4921 taacggcagg acaactgggc cggcagtaga tcccggtcga agtaacagcg acgtaaaacc
     4981 acccgtcgaa gcggacgtcg cgcccgcgca cggccctgta gcgatgttcg aagtccttga
     5041 gcacgccctg attgtcgcat ccgaaagaca cgattactgg cggaaatcgg acatgggcat
     5101 catcatagca tcacgagcgg caggacgagc tcgtagccct gccgctcttg gctggagagg
     5161 gcttcggcga ccggttctgc atcgcatggt gcataattag gctgtctcgc ggaggagata
     5221 ggaggataag ggttgatagg tggtctgctc gccgaactcg tacggtgggt atttgacgta
     5281 gtccactctt tcgggtacgt cggcatattc gtactca
//
LOCUS       DATAIR010000288         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_110844, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000288 DATAIR010000000
VERSION     DATAIR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110844"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /gene="rplE"
                     /locus_tag="VHM69_07035"
     CDS             <1..126
                     /gene="rplE"
                     /locus_tag="VHM69_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2740185.1"
                     /translation="EISYDSIDATRGLDVAVVTTTESDEEARELLRLLGMPFRQS"
     gene            137..322
                     /locus_tag="VHM69_07040"
     CDS             137..322
                     /locus_tag="VHM69_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2740186.1"
                     /translation="MTRKALLVKQQRKQKYAVREYNRCQRCGRAHGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            329..727
                     /gene="rpsH"
                     /locus_tag="VHM69_07045"
     CDS             329..727
                     /gene="rpsH"
                     /locus_tag="VHM69_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2740187.1"
                     /translation="MTVTDPIADFLTRIRNAHMAKQETVEIPHSKMKAEIARILQEEG
                     YVQDVSERGSGVEKRLVINLKYGPDGRRGITGLRRMSRPGRRVYRKQTSIPRVLDGLG
                     VAILSTSQGVLTDHQARRRGVGGEVLCFVY"
     gene            742..1275
                     /gene="rplF"
                     /locus_tag="VHM69_07050"
     CDS             742..1275
                     /gene="rplF"
                     /locus_tag="VHM69_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2740188.1"
                     /translation="MSRIGKAPVEVPGAVNVELSGRSVKVTGPKGELTVPVGRGVEVK
                     QEDGNVIVERASDTPEHRAMHGLTRSLIQNAVTGVTDGFTKTLNIAGVGYRANLQGQN
                     VNLQVGYSHPVSISPREGIEFEVPNATTIIVRGIDKQRVGQTAAEIRMVRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            1287..1634
                     /gene="rplR"
                     /locus_tag="VHM69_07055"
     CDS             1287..1634
                     /gene="rplR"
                     /locus_tag="VHM69_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2740189.1"
                     /translation="MAVLQKRAHREKRHRRVRRKVVGTATRPRLSVYRSNVHIYAQLI
                     DDDAGSTIAAADSREVQAENRKDAARKVGELIANKASDAGIEVAVFDRGGNKYHGRIA
                     ALAEGAREGGLKL"
     gene            1651..2196
                     /gene="rpsE"
                     /locus_tag="VHM69_07060"
     CDS             1651..2196
                     /gene="rpsE"
                     /locus_tag="VHM69_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2740190.1"
                     /translation="MGRPRSDNRQGGRGQGGRGGRQRDGASDVQDRVVEIRRVAKVVK
                     GGRRFNFSALVVVGDGKGRVGAGLGKANTVPAAIQKGQERAKRNMFDVPMRNTTIPHE
                     VLGKFESSSVLLKPASEGTGVIAGGGVRAVLELAGIKDVLTKALGSTTSVNLVQATVE
                     GLQRLRSKAEIEQMRGVKITL"
     gene            2193..2375
                     /gene="rpmD"
                     /locus_tag="VHM69_07065"
     CDS             2193..2375
                     /gene="rpmD"
                     /locus_tag="VHM69_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2740191.1"
                     /translation="MSPLKVTQVRSVIGSKQGHKRTVRALGLKRIRDSRVHQDTPQIR
                     GMVHKVQHLVHVEEVE"
     gene            2378..2839
                     /gene="rplO"
                     /locus_tag="VHM69_07070"
     CDS             2378..2839
                     /gene="rplO"
                     /locus_tag="VHM69_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2740192.1"
                     /translation="MRLNDLSPAPGSKKARKRVGRGTGSGHGKTSGRGHKGAGARSGA
                     SAFAGYEGGQMPLQRRLPRLKGEARGRHTVARPTVYAPVNLGELDGVPGDTIGPDELR
                     AAGLVRKKAELVKILGNGDLGRAVTVRAHAFSRSAREKIEAGGGSVEILER"
     gene            2842..>3566
                     /locus_tag="VHM69_07075"
     CDS             2842..>3566
                     /locus_tag="VHM69_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2740193.1"
                     /translation="MLGSLANAWKIPELRQRLLFTAAMIAAYRLGAYIPLPGVNPEIL
                     GSGLQTNNPITGLFGTFTGGAFNHFSVFSLGIMPYITAAIVMQLMTVAIPRLQELAKE
                     GETGQQKITQYTRYFTLALALIQSVAMVFFFRSAQGGGALAGTSPIEILVAVVTLTAG
                     VMLTMWLGELISQRGLGNGISLIITASILSQAPRAIQSLIENGDVLTMVILGLLAVMI
                     VAAIVFVNEGQRRIPITYAKRQVG"
BASE COUNT          726 a         1065 c         1240 g          535 t
ORIGIN      
        1 gagatctcct acgactccat agacgcgacc cgtgggctgg acgtggcggt cgtcacgacg
       61 acggagagcg acgaagaggc gcgggagcta ctacggctcc tcgggatgcc gttcaggcag
      121 agttgagagg tagggtatga cgagaaaggc gttgctggta aagcagcaga gaaagcagaa
      181 gtacgcggtc cgggagtaca accggtgcca gcggtgtggc cgggcgcacg gttactacag
      241 gaagttcggg ctgtgccgga tctgcctcag ggagctggcg cacgagggga agatccccgg
      301 cgtaacgaaa gcgagttggt agggaaatat gacggtcaca gatcctatag cggatttcct
      361 gacgcgcatc cgcaacgcgc acatggcgaa gcaggagacg gtggagattc cccattcgaa
      421 gatgaaggcg gagatcgccc gaatccttca ggaggagggc tacgtccagg acgtctcgga
      481 gcgcgggagc ggggttgaga agaggctcgt catcaacctg aagtacggcc ccgacggtcg
      541 tcgcggcatc acgggcctga ggcggatgag ccgccccgga cgccgggttt accgcaagca
      601 gaccagcatc ccccgcgtgc tcgacgggct cggggtggcg atactctcga cctcccaggg
      661 ggtcctgacc gaccaccagg ccaggcgcag aggcgtcggc ggcgaagtcc tgtgcttcgt
      721 ctactaggga ggtattgagg gatgtcaaga atcgggaagg ctccggtcga agtcccggga
      781 gcggtgaacg tcgagctctc gggccgcagc gtgaaggtga cgggcccgaa gggcgagctc
      841 acggtgcccg tcggacgcgg ggtcgaggtc aaacaagagg acggcaacgt aatcgtcgag
      901 cgggcctcgg acacgccaga gcacagggcg atgcacgggt tgacgcggtc cctgatccag
      961 aacgccgtca ctggcgtgac ggacgggttc acgaagacgc tgaacatcgc gggcgtcggc
     1021 tacagggcga acttgcaggg gcagaacgtc aacctgcagg tcgggtactc gcacccggtc
     1081 tcgatctcac cccgcgaggg gatagagttc gaggttccca acgcaacgac catcatcgtg
     1141 cgcgggatag acaagcagcg ggtaggccag acggcggcgg agatcaggat ggtccgtccg
     1201 cccgagccgt acaagggcaa gggcatccgc tacagcgacg agcagatccg gcgcaaggtc
     1261 ggcaaggccg gatagtagga ggattgatgg ctgtacttca gaagcgggcg cacagggaga
     1321 agcggcacag gcgcgtcagg cgcaaggtgg tcggcacggc cacccggccg cgtctctccg
     1381 tgtaccgatc gaacgttcat atctacgccc agctgatcga cgacgacgcg ggaagcacca
     1441 ttgctgctgc ggactcccgt gaggtccagg ccgaaaatcg caaagacgcg gcccgcaagg
     1501 tgggcgagtt gatcgccaac aaggcgtcgg atgcggggat cgaggtcgct gtcttcgata
     1561 ggggcggcaa caagtaccac ggacgcatcg cggcgctcgc cgagggcgcg cgagaaggcg
     1621 gactgaagct ctaatacagg aggaaacgtc ttgggaagac caagaagcga taacagacaa
     1681 ggcggacgcg gacagggcgg ccgtggaggc cggcaacgcg acggcgcctc tgacgtccag
     1741 gacagggttg tcgagatccg ccgcgtcgcc aaggtcgtca agggcggccg gcgcttcaat
     1801 ttctcggccc tcgtggtcgt cggcgacggc aagggcaggg taggcgcggg cctcgggaag
     1861 gccaacacgg tccccgccgc catccagaag ggccaggagc gggccaagcg caacatgttc
     1921 gacgtgccga tgcgcaacac aacgatcccg cacgaggtcc tcggcaagtt cgagagctct
     1981 tcggtgctcc tcaagccggc ctccgagggt actggagtta tcgcgggcgg cggagtgcgt
     2041 gccgtcctcg aactcgcggg gattaaggac gtgctcacga aggcgctcgg ctcgaccacc
     2101 tctgtgaacc tggtccaggc cacggtcgag ggcctccaga ggctgcgcag caaggcggag
     2161 atcgagcaga tgcgaggggt gaagatcaca ctatgagccc cctgaaggta acgcaggtaa
     2221 ggagcgtcat aggctccaag cagggtcaca agcggacggt gcgcgctctg ggtctgaagc
     2281 gcattagaga ctcccgggta catcaggaca caccccagat ccggggcatg gtccacaagg
     2341 tgcagcacct cgtccacgtc gaggaggtag agtagctgtg aggctgaacg atctctcccc
     2401 tgccccaggc tcgaagaagg cgcgcaagcg cgtcggacgc ggcaccgggt cggggcacgg
     2461 caagacttcg gggcgcggcc acaagggcgc cggcgcccga tccggcgctt ccgcgttcgc
     2521 cggctacgag ggtggacaga tgccgctgca gcggcggttg cctcgactca aaggcgaggc
     2581 gaggggccgt cacacggtag cccgcccgac cgtctacgcg ccggtgaact tgggcgaact
     2641 agatggcgtc cccggtgata cgataggtcc tgacgagctg cgggccgccg ggctcgtcag
     2701 gaagaaggcc gaactcgtca agatcctcgg caacggggac ctcggacgcg ccgttaccgt
     2761 cagggcgcac gcgttctcca ggtcggcgag agagaagatt gaggcgggcg gcggaagcgt
     2821 cgagatactg gagcggtaga gatgttggga tccctagcca acgcctggaa aatcccggag
     2881 ctgcgacaga ggctcctgtt cacggctgcc atgatagccg cctacaggct cggggcgtac
     2941 ataccccttc ccggggtcaa ccccgaaatc ctcggtagtg gtctccagac caacaacccc
     3001 ataacgggcc tgttcgggac cttcaccggc ggggccttca accacttctc ggtgttctcg
     3061 ctcgggatca tgccttacat cacagcggcg atcgtgatgc agcttatgac ggtcgccatc
     3121 ccccgtttgc aggaactggc caaagagggc gagacggggc agcagaagat cacacagtac
     3181 acccgctact tcacgctcgc gctcgcgctc atccagtccg tcgcgatggt cttcttcttc
     3241 cgttcggcgc agggcggcgg cgcgctcgca gggacgagcc ctatagagat actcgtcgcc
     3301 gtagtcaccc ttacggcggg cgtgatgctc acgatgtggc tcggcgagct cataagccag
     3361 cgcggcctcg gcaacggcat ttcgctcatt attacggcct cgatcctctc ccaggcgccg
     3421 cgcgccatac agtctctcat cgagaacggg gatgtcctga ccatggtgat cctcgggctg
     3481 ctcgcggtca tgatcgtcgc cgccatcgtc ttcgtcaacg agggccagcg caggatcccg
     3541 atcacctacg caaagcgtca ggtcgg
//
LOCUS       DATAIR010000289         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_147373, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000289 DATAIR010000000
VERSION     DATAIR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147373"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VHM69_07080"
     CDS             complement(<1..1580)
                     /locus_tag="VHM69_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2740194.1"
                     /translation="MTSDGTGREDGAMGERRFLAGVALDNLPARVAIIDESGAIVAVN
                     RAWHEFAATNDGAPIATEDVNYLRACEAAKGDGAREAARFAEGVRSVLSGQRQEFTMD
                     YPCHSPGERRWFVGWVARLLVGDARWAVISHEDITGRKLAEEELLRSETRNKAMLEAV
                     PDLMFLISRDGEYLDFRARDEDKLYVPPEEFVGRNLREIMPPELAGPMMDGIARALDT
                     GETQLIEYNLPMPEGVRDFETRLVASGPEEILSVVRDVTGRKVAERKLRESEERFRLL
                     AENARDVIFRYRLDPALGYDYVSPSVEALTGYAPEEFYADPNLAIRLIHPEDRPLVET
                     IFKAPESPLLLRCRHRDGRLVWIEQRNKPVRDESGNLVAIEGIARDVTRRKMADRERE
                     QFLDRERAAHQHLEAILDNLGEGVLVIGSGESVLFANPSAQVMLGMTDDYTHAELPDP
                     CEGFSLRDAVDKCIRTGETIEARARCGGKFLQIRLDRLSGPKANEVLVVLQDLSEGHR
                     LEANQQRFLSNAAHQLKTP"
BASE COUNT          282 a          572 c          498 g          354 t
ORIGIN      
        1 ggcgtcttga gctgatgggc ggcgttggac aagaaacgct gttggttggc ctccagccga
       61 tgcccttccg agaggtcctg caaaaccacg agcacctcgt tcgccttcgg tccagataaa
      121 cggtccaacc tgatctgaag gaacttccca ccgcaccgcg cccgggcttc tatcgtctcc
      181 ccggtccgga tgcacttatc caccgcgtcc cgcaggctga aaccttcgca agggtccggt
      241 aattccgcat gtgtatagtc atccgtcatg cccaacatga cctgtgctga ggggttggca
      301 aagaggaccg attccccaga cccaataacg agtacgccct cgccgaggtt gtccagtatg
      361 gcttcgaggt gctggtgagc cgctcgttct cgatccagga actgctcgcg ctccctgtcg
      421 gccatctttc ttcgggtcac gtcgcgggcg atgccctcga tggccaccag gttgcctgat
      481 tcgtcgcgta ccggcttgtt gcgctgctcg atccagacca ggcgtccgtc cctgtgtctg
      541 cagcgtagca gcaggggtga ttcgggcgcc ttgaatatgg tctcgaccag cggccggtct
      601 tccggatgaa ttagccggat agccaggttc ggatcggcgt agaactcctc gggggcgtag
      661 ccggtaagcg cctcgacgga aggacttaca tagtcgtacc caagcgctgg gtcgagccgg
      721 tagcggaaga tgacgtccct ggcgttctcg gccagaagcc ggaagcgctc ctcactctcg
      781 cgcagcttcc gctcagcaac cttgcgtccg gtcacatcgc gcacgacgct caggatctct
      841 tccggaccac tggccacgag ccgcgtctcg aagtcccgca cgccctccgg catggggagg
      901 ttgtattcga tgagttgcgt ctcgcccgtg tcgagcgccc tggcgatgcc atccatcatc
      961 ggcccggcca gttcgggcgg cattatctcg cgcaggttgc ggccgacgaa ctcttctggc
     1021 gggacgtaca gcttgtcttc gtctctggcc cggaagtcca ggtactcgcc gtcgcggctg
     1081 atgaggaaca tcaggtcagg gacggcctcg agcatcgcct tgttcctggt ctcgctcctc
     1141 agtagctctt cctcggccag cttgcgtccg gtgatgtctt cgtgcgagat cacggcccac
     1201 cgcgcgtcgc caacgagaag gcgcgcaacc cacccgacga accagcgccg ttctccgggg
     1261 gagtggcaag gatagtccat ggtgaactcc tggcgttggc ccgagagcac cgaacgtacc
     1321 ccctccgcga agcgagcagc ctcccgagcc ccgtcaccct tcgccgcttc gcaggcccgg
     1381 aggtagttga cgtcctccgt cgctatgggg gcgccgtcat tggtcgcggc gaactcatgc
     1441 cacgccctgt tcacggcgac tatggctccg gactcgtcga tgattgccac tcgcgccggt
     1501 agattgtcga gcgctacgcc ggcgagaaac ctgcgctcgc ccatcgcccc atcttcgcgg
     1561 cccgtcccgt cagaggtcac actgctacca ccactcgtct ttccggctga tcgaacgcga
     1621 cagtctcctc cttcgactcg ccggctgaag tgcatttgtt cttagatagt atctaatctt
     1681 accgccgttc catcccgatt tatagg
//
LOCUS       DATAIR010000290         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_387750, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000290 DATAIR010000000
VERSION     DATAIR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387750"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..424
                     /locus_tag="VHM69_07085"
     CDS             56..424
                     /locus_tag="VHM69_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX2740195.1"
                     /translation="MRDFEQLKVWERSHRLTLVVYEATAAFPKEEKYGLKSQIRRSCA
                     SVPANIAEGCGRGSNTDLARFLQMSLGSASELQYHLRLACDLSYLNHHAYERLTEEVI
                     EIKRMLTSFIDNLRAFASQT"
     gene            471..2015
                     /gene="zwf"
                     /locus_tag="VHM69_07090"
     CDS             471..2015
                     /gene="zwf"
                     /locus_tag="VHM69_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2740196.1"
                     /translation="MADSLKTESLQNILRDEQRLPRVPEPGVMVIFGASGDLTSRKLV
                     PALYDLAAQRRLPLEFAVVGISRTEMSHEDFRGKLRKALEEHRPGEVSDDVWESFAGG
                     IFYLPGDSMKTETFDELKQFLQQLDSERGTQGNRAFYLSSSPSLFPAIVEHLGEAGLN
                     QGEDGGWSRLVVEKPFGRDLESARELNAELQRFFDETQIYRIDHYLGKETVQNILALR
                     FANGIFEPIWNQHYVDHVQITVAEDIGVGTRGAFYEEAGALRDIVQNHVMQVLCLTAM
                     EPPVAFDAESVREEKVKVLKAVRPIPEDDVENYAVRGQYERGWVWGEEVPGYRKEKNV
                     DPESVTETYVALKVFVDNWRWAGVPFYIRASKRMPKKATEVAIQFHSSPHTPFAHSDT
                     EGLEPNVLVVRVQPEEGLSLKIGAKVPGTGFEVSSVNMDLLYGTAFLEEAPDAYQRLL
                     LDLMLGDPTLFIRADEAEGAWKILDPVMNHWSQKREVSFYPAGTWGPEEADALLERDG
                     REWRRP"
     gene            2012..3085
                     /locus_tag="VHM69_07095"
     CDS             2012..3085
                     /locus_tag="VHM69_07095"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2740197.1"
                     /translation="MIGESRQAPGGGRMSVAQIEHELAKLRTNDDGTLALRSSVLNLI
                     VVTDEDSATEITNSVSKLAGRYPARAVVFIADPDGESNVDVRLSAFCNVRGGGAHVCA
                     EQVTIHAEGPPATHLESLAGPLLIPDLPVFLWYPGEFSPSSPEFAAMAELADRIIVDS
                     AATERGEGCLREISALLEDPEAPAIGDLQWVGLSPWRSLLADTFDAPERAGELQKINR
                     LEVLYAPGGENRALLLIGWLASTLGWKAESAASRDGGARLLGFSGPPGDISVELSPGS
                     PDARLRRIRLYSEEYSFQVSRHRALSDVRTTVMRGEDLLAERTVHLGSFDLGVLVGEE
                     LSYRGHDEAYEGALRAAVEVLDL"
     gene            3082..>3544
                     /gene="pgl"
                     /locus_tag="VHM69_07100"
     CDS             3082..>3544
                     /gene="pgl"
                     /locus_tag="VHM69_07100"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2740198.1"
                     /translation="MSVRVYESPEKLAEAAAREFATKAAEAIEERGRFAVVLAGGSTP
                     RATYKILASDYSDSIDWSKVHVFFGDERTVAPDHEDSNYRMAREALLDHIRVGSVHRM
                     RCELLPDEAAADYEEQLREFFGGLPQFDLILLGIGGDGHTASLFPETPALEV"
BASE COUNT          672 a         1131 c         1135 g          606 t
ORIGIN      
        1 caggcatcag gcatcaggct tcaggtaaag gggcatgcag ccgattactt tgggtatgcg
       61 cgatttcgag cagctcaaag tgtgggagag gagccaccgg ttgacgctgg ttgtttacga
      121 agccaccgcg gcctttccca aggaagaaaa atacggcctg aagagtcaga tacgccgatc
      181 ctgtgcctct gttcccgcca atattgccga aggttgcggc agaggcagca acacagatct
      241 ggcacgcttt ctccagatgt cactgggttc tgcgagcgag cttcagtacc atcttcgcct
      301 cgcctgcgac ctctcgtatc tgaaccacca cgcctacgaa cgtttgaccg aggaagtgat
      361 cgagataaag cgtatgctca catcctttat cgacaacctc agggccttcg ccagccagac
      421 ttgaaacctg aaacctgttg cctgaagcct aaagtgaaag ctgaccgctg atggctgaca
      481 gcttgaagac cgaaagtcta caaaacatcc tgagagacga gcagcgtctc cctcgcgtac
      541 ccgagcccgg ggtcatggtg atcttcggcg cgtcggggga cctgacctcc cgcaagctcg
      601 tccccgccct ctacgacctc gccgcccagc gcaggcttcc gctcgagttc gctgttgtcg
      661 gcatctcgcg gaccgagatg agccacgagg atttcagggg gaagctgcgc aaagcgcttg
      721 aggagcaccg ccccggagag gtctccgacg acgtctggga gtccttcgcc ggcggcatct
      781 tctatctccc cggcgactcg atgaagaccg agacattcga cgagctcaag caattcctgc
      841 agcagctcga ctccgagcga gggacgcagg ggaacagggc tttctatcta tcgtcttcgc
      901 cttcgctctt ccccgccatc gtggagcacc tgggcgaggc gggcttgaac cagggcgagg
      961 acggcggctg gtcgcggctc gtcgtggaga agcctttcgg gcgggacctg gagagcgcca
     1021 gggagctaaa cgcagagctt cagcggttct tcgatgagac ccagatctac cgcatcgacc
     1081 actacctcgg caaggagacg gtccagaaca tccttgcctt gcggttcgcc aacggcatct
     1141 tcgagccgat ctggaatcag cactacgtcg accacgttca gatcacggtc gcggaagata
     1201 tcggcgtcgg tacccgcggc gcgttctacg aggaagccgg cgccctgcgc gatatcgtcc
     1261 agaaccacgt gatgcaggtc ctgtgtctta cggcgatgga gccgccggtc gccttcgacg
     1321 ccgagtcggt gcgcgaggag aaggtgaagg tactgaaggc ggtccgcccc atccccgagg
     1381 acgatgtcga gaactacgct gtgcgcgggc agtacgagag gggttgggtg tggggcgagg
     1441 aggtccccgg ctaccgcaag gagaagaacg tagatcctga gtccgttacc gagacttacg
     1501 tggcgctcaa ggtgtttgtg gacaactggc ggtgggccgg ggtcccgttc tacatacggg
     1561 ctagcaagag gatgccgaag aaggcgacgg aggtcgcgat ccagttccac tcctcgcccc
     1621 ataccccctt cgcccacagc gacacagagg gccttgagcc gaacgtgctc gtcgtccgcg
     1681 tccagccgga ggagggcctg tcactaaaga tcggggccaa agtcccaggc acaggcttcg
     1741 aggtcagctc ggtgaacatg gacctgctct acggcactgc cttcctcgag gaagcccccg
     1801 acgcgtacca gcgcctccta ctggacctga tgctcggcga ccccaccctg ttcatccgcg
     1861 cggacgaggc cgagggtgca tggaagatac ttgaccccgt gatgaaccac tggtcacaga
     1921 agcgggaggt gtctttctat ccggccggga cctggggacc ggaagaggca gacgcgcttc
     1981 tcgagcgcga cggaagggag tggaggaggc cgtgatcggg gaatcccgcc aggcccccgg
     2041 aggcggacgg atgagcgtcg cgcagataga gcacgagctt gccaagctcc gcacgaacga
     2101 cgacgggacg ctcgccctcc gctccagcgt gctcaacctg atcgttgtga cagacgagga
     2161 ttcggccacc gagatcacga actcggtctc gaagctggcg ggccgctatc ccgcgcgggc
     2221 ggtcgtgttt atagctgatc ctgatggcga atcgaacgtg gacgtgcggc tctcggcctt
     2281 ctgcaacgtg aggggcggcg gcgcgcacgt ctgcgccgag caggtgacga tccacgccga
     2341 aggtccccca gcgacgcatc tggagagcct cgcgggcccg ctcctgatcc ctgacctccc
     2401 cgtcttcctg tggtacccgg gcgagttctc gcccagttca ccagagttcg ccgcgatggc
     2461 cgaattggcg gatcggatca tagtggactc cgccgcgaca gagagggggg aaggttgcct
     2521 gcgcgagata tcagccctgc tcgaagaccc cgaggcaccg gccatcgggg atctgcagtg
     2581 ggtcggactc tcgccatggc gctcccttct cgctgatacg ttcgacgccc cggagcgcgc
     2641 cggagagctc cagaagatca accgcctgga ggtcctctac gcgcctggcg gggagaaccg
     2701 cgcgttgctc ctgatcggct ggctcgcctc caccctggga tggaaggccg agagcgcggc
     2761 gagccgcgac ggtggcgccc gcctgctagg gttctcgggt ccccctggag acatctccgt
     2821 cgagctctct cccggctccc ccgacgccag actcaggcgc atccggctct actcggaaga
     2881 gtacagcttc caggtctcgc gccatcgcgc cctctctgac gtccgcacca cggtaatgcg
     2941 cggcgaggat cttctcgccg aacgcacagt ccacctcggc tcgttcgacc tcggcgtcct
     3001 ggtcggagaa gaactgagtt accgaggtca cgacgaagcc tacgagggcg ccctccgcgc
     3061 ggctgttgag gtcctcgacc tttgagcgtt cgcgtctacg agagcccgga gaagctcgca
     3121 gaggccgccg cgcgcgagtt cgcgacgaag gccgcggagg ccatcgagga gcgcgggcgt
     3181 ttcgccgtcg tgctcgccgg cggctccacc ccgagggcga cctacaagat actggccagc
     3241 gactactccg acagcataga ctggagcaag gtccacgtct tcttcgggga cgagcgcacg
     3301 gtcgcgcccg accacgagga ctcgaactac aggatggccc gcgaggcgct gctcgaccac
     3361 atccgcgtcg ggagcgtcca caggatgcgg tgcgagctac ttccggatga agcggcagcg
     3421 gactacgaag aacagttgcg ggagttcttc ggaggacttc ctcagttcga cctgatcctg
     3481 ctgggcatag gcggcgacgg ccacaccgca agcctcttcc cggagacccc ggcgctggag
     3541 gtcc
//
LOCUS       DATAIR010000291         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_434071, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000291 DATAIR010000000
VERSION     DATAIR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434071"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /gene="rpoB"
                     /locus_tag="VHM69_07105"
     CDS             complement(<1..1550)
                     /gene="rpoB"
                     /locus_tag="VHM69_07105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2740199.1"
                     /translation="MQEAFRIGMYRMERVVRERMTTQDEESITPQTVVNTKPVASAIK
                     EFFGSSQLSQFMDQTNTLSGLSHRRRLSAMGAGGLSRERAPIEVRDVHPTHYGRMCPI
                     ETPEGPNIGLIGQLSTFATVDDYGFVQTPYRKVVNGKVTDEIEYLSADEEEEFTIAQA
                     NTPVDQQSGKIAEGAQILARRKGGDVATVAAEEVDYVDVAPLQTVSVGTALIPFLEHD
                     DANRALMGANMQRQAVPLLRSEAPYVGTGMEFRAAADTGDVLLARHAGTVERVVSDKI
                     TVRREGGELDEYFLRKFARSNQGTCVNQRPLVTEGEEVEAGTVMADGSSTDQGELALG
                     KNLLVAFMPWEGFNFEDAIIISERIVKDDTLSSIHIEEYEVQARDTKLGPEEITRDIP
                     NASDDVLMNLDVDGIIRIGAEVGSGDVLVGKVTPKGESEPTPEEKLLRAIFGEKAREV
                     KDSSLKVPHGEGGVVIDVKRFSRDGGDELQPGVNEMVRVFVAKKRKIAEGDKLAGRHG
                     NKGVISRIV"
BASE COUNT          240 a          535 c          479 g          315 t
ORIGIN      
        1 acgatcctcg agatgacgcc tttgttgccg tggcgcccgg cgagcttgtc gccctcggcg
       61 atcttgcgct tcttggccac gaagaccctg accatctcgt tgacgcctgg ctgtagctcg
      121 tcgccaccgt ctcgactaaa gcgcttgacg tcgatgacga cgccgccctc gccgtgggga
      181 accttgaggg aagagtcttt gacctcacgc gccttctcac cgaagatcgc gcgcagcagc
      241 ttctcctctg gcgtcggctc ggactcgccc ttgggcgtga ccttgccgac cagcacgtcc
      301 ccggaaccta cctcggcgcc gatacggatg atgccgtcaa cgtccaggtt catcaggacg
      361 tcgtcggaag cgttggggat gtcgcgggtg atctcctcgg gacccagctt cgtatcccgc
      421 gcctgtacct cgtactcctc gatgtgaatg gaggagaggg tgtcgtcctt gacgatccgc
      481 tcggagatga taatggcgtc ctcgaagttg aacccctccc acggcatgaa agccacgagc
      541 aggttcttac caagggcaag ctcgccctgg tccgtcgatg agccgtcggc catgacggtc
      601 cccgcctcga cctcctcgcc ctcggtgacg agcgggcgct ggttgacgca ggttccctgg
      661 ttggagcggg cgaacttcct caggaagtac tcgtcgagct cgccgccctc gcgacgcacc
      721 gttatcttgt cggagacgac ccgctcgacg gtgcctgcgt ggcgagccag caacacgtct
      781 ccggtgtcgg ccgcggcccg gaactccatc ccggtaccca cgtacggagc ctcgctcctc
      841 aggagcggta cagcctggcg ctgcatgttc gcgcccataa gcgccctgtt ggcgtcgtcg
      901 tgctcgagga acgggatcag ggccgtgccg acggagaccg tctgcagtgg cgccacgtcg
      961 acgtagtcca cctcttccgc ggccacggta gcgacgtcac cgcccttcct gcgggccagg
     1021 atctgggctc cctcggcgat cttgccgctc tgctggtcaa cgggcgtgtt ggcctgcgcg
     1081 atcgtgaact cctcctcctc gtcggcggag aggtactcga tctcatccgt caccttgccg
     1141 ttgacgacct tcctgtaagg cgtctgcacg aagccgtaat cgtccacggt ggcgaacgtc
     1201 gaaagctggc cgatgagccc gatgttcggg ccttcgggcg tctctatggg gcacatcctg
     1261 ccgtagtgcg tcggatgcac gtcgcgcacc tctatgggcg cccgctccct gctgagccca
     1321 ccagctccca tcgctgagag ccggcgcctg tgcgagagac ctgagagcgt attagtctgg
     1381 tccatgaact gcgagagctg cgaggagccg aagaactcct tgatcgcgct cgccacaggc
     1441 ttggtgttga cgacggtctg gggggttatg gactcctcgt cctgtgtcgt catccgctcg
     1501 cgcaccacgc gctccatgcg gtacatcccg atccggaacg cttcctgcac caactctccg
     1561 acggtcctg
//
LOCUS       DATAIR010000292         3946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_193009, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000292 DATAIR010000000
VERSION     DATAIR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193009"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHM69_07110"
     CDS             <1..228
                     /locus_tag="VHM69_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740200.1"
                     /translation="DTQSGEIMEEGRLRTTPEVFRRRFSSEQPMRIAIETGTHSPWVS
                     RVLEECGHEVLVANARKLRLIYARASARPTR"
     gene            249..1001
                     /locus_tag="VHM69_07115"
     CDS             249..1001
                     /locus_tag="VHM69_07115"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2740201.1"
                     /translation="MARVDPKLLYPLKHRGEESQAHMGLIRSRQALVDCRTQLVNHVR
                     GAVKSFGARLPKCPARSFHKRASEHIPDALRPALGPILEQIGSLTERIHQYDRKLETL
                     CEEHYPETELLRQVEGIGSLTALTFVLTLENPHRFEKSRSVGAYLGLVPATDRSGERD
                     PQKRISKEGDEMLRKLLVSSAHYVLGPFGSDSDLRRHGEKIAARGGKNAKKRAVVAVA
                     RKLSVLLHRLWVTAETYDPLHHAHRCQQKGAA"
     gene            1334..1555
                     /locus_tag="VHM69_07120"
     CDS             1334..1555
                     /locus_tag="VHM69_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740202.1"
                     /translation="MPSHRRRIQSFIDRRFYRSKYDARRTLEAFSVKLRDVTDLNSLN
                     SELLSAVRDAMQPEHVSLWLCPEVVRRDE"
     gene            2152..2811
                     /locus_tag="VHM69_07125"
     CDS             2152..2811
                     /locus_tag="VHM69_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2740203.1"
                     /translation="MNDTSSDYASRGHAGFLHLLSCGDPTMASLVATIGPLSIEQRRR
                     GRPDDAYGSLVRSIVGQQLSTVAARTIYGRLTSLFDHRPPTPAELLAADEETLRACGL
                     SRPKISYLRDLARHVLEDGLDLPRLRELPDDEVASRLVAVKGIGQWSADMFLMFHLTR
                     PDVLPVGDLGIRRAVERAYGLDGLPDAETLREIAVPWRPQRTLACLYLWGSLDNVPGS
                     G"
     gene            complement(2874..>3946)
                     /locus_tag="VHM69_07130"
     CDS             complement(2874..>3946)
                     /locus_tag="VHM69_07130"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740204.1"
                     /translation="RTQRKKQLLALFAYIGAISTMGLYLIQGSAYLFGGMLFVIANLS
                     FGASIVVYNAFLPEIAEPDERDRVSSRAWALGYLGGGLLLAANLALFTVHESLGLSAG
                     HAVRISLLSAGAWWALFTLIPLRTLRDRGPVHAGEQGAAVVTAGFRQLLGTLRASRAY
                     PQTLLFLAAYLLYNDGIQTVITLTAVYGEEELKFGTSTIISVILMVQFVAFLGALLLG
                     MCARFFGAKRVVLASLILWTGAVAVAYGLEAGAVWQFYLLATFIGIVLGGSQALSRSL
                     FSHMIPRGQEAEYFSLYEISERGTSWLGPLLFGLTLQLTGSYRSALASLVIFFFVGFV
                     LLALVNVRRAIVEAGNTPPERV"
BASE COUNT          846 a         1235 c         1195 g          670 t
ORIGIN      
        1 gacacacaaa gcggcgagat tatggaagag ggcaggctgc gcaccacccc cgaagtcttc
       61 agacgacgct tctcctccga gcagcccatg cgtatcgcga tagaaacagg aacccactcg
      121 ccttgggtga gcagggtgct cgaggagtgt ggccatgagg ttctggtagc caacgctcgc
      181 aaattgcggc tcatctacgc cagagcaagc gcaagaccga cgaggtagat gccgagaacc
      241 tggcccgcct ggctagggtg gatccgaagc tcctgtatcc gctcaagcac cggggcgaag
      301 agagccaggc ccacatgggc ctgatccgct cgaggcaggc gttggtggat tgccgcacgc
      361 aactcgtgaa ccacgttcgg ggagccgtga agtccttcgg ggctcgcctg cccaagtgcc
      421 cggcaaggag cttccacaag cgagcgtccg agcacatccc cgatgcgctt cggccagccc
      481 ttgggcccat cctcgagcag atcggctcgc tcaccgagcg tatccatcaa tacgaccgaa
      541 agctggaaac gctctgcgaa gagcattacc ccgaaacgga gctcctgcgc caggtcgagg
      601 ggatagggtc gctgacggcg ctcacgttcg tgctcaccct ggagaacccc caccgcttcg
      661 aaaagagccg ctcagtgggg gcgtacctgg ggctcgtgcc agccaccgac cgctcgggag
      721 agagggaccc ccaaaaacgc atatccaagg agggcgacga gatgctgagg aagcttctgg
      781 tctcaagcgc ccactacgtc ctgggtccct tcggctccga ctcggacctc cgtcgccacg
      841 gagagaagat agccgcccgt ggcggcaaga acgcaaagaa gagggcggtg gtggccgtag
      901 cgaggaagct ctcggtgctc ttgcaccgcc tgtgggttac ggcagagacc tacgatccgc
      961 tccaccacgc acatcggtgc caacagaagg gggctgcttg aacagaggcg atgggccaga
     1021 cgagcgcaga ggaggtgaag atagcgctac ggcagccagg ctagcctaga gtggcagata
     1081 gccacggctg atctcgcccg aacgcgctcc tgatccgaag gcgactgcgc caagcgaact
     1141 tggtccatcc tcaaagagga gagaccgctg agaagaagat tgcagcgcct ttggctcgaa
     1201 aagacggatc tcatcctatg caccgagcca cagaagagag ggctccttca gagtgcggat
     1261 agaagcatag cgcgaagggc agaacagttg gcgttcacac cacacaacgg ccgccacgtc
     1321 ggcttgacaa acagtgcctt ctcatagaag acgcatccag tcgttcatag accgccgctt
     1381 ctaccgcagc aagtacgacg ccagaaggac cctggaagcc ttctccgtca agttgcgcga
     1441 tgtaacggac ctgaacagct tgaacagcga acttctgtcc gcggttcggg acgcgatgca
     1501 gcccgagcac gtatccttgt ggctgtgtcc cgaggtggtc cgtagggatg agtaagcaga
     1561 ctcgccgccg tcttattcac ccaacgtcgg ggaagggaaa ttctcagaat tccatatcca
     1621 ggatcctgta ttggccgcgt ccatcgggcc gggttttcct cctgtttccc ggccccacgc
     1681 attctagtca acgcaccatg tagtagcgct ggatagatat gcgccatctt gtatgtcgct
     1741 cggggtgttg ctccgatccc tgcatcttga cccgctgcac ttcgttgaac gtgtagtgct
     1801 gaggtggcac tccaattacg tgacgtgcag cagctttttg atggtgagat tctggaggtg
     1861 cggataatcc tgcactcatc ccgacgagtt gtagaagcct atgctaaggc tgccatcctc
     1921 atgtcttggc taacttttca ttactccaca aatccttcgg ctcaactgag aggttccctt
     1981 cccacaaaga ttgtagggta catgttactg atgaaggata ataaaggaaa gcgggccggg
     2041 cttcggcaaa aaattctcta gaaggaaggt atctaatgac gaaggcattc ggcgcaaacc
     2101 accgggcgcg tcgagctttt cagcaactga gctagtcgac caggagaacg catgaacgac
     2161 accagcagcg attacgcctc ccgcgggcac gcgggctttc tgcacttgct ctcctgcggt
     2221 gacccgacga tggcctcact tgtggcaact ataggtccgc tcagcatcga gcagcgtcgc
     2281 cgtggacggc cagacgacgc ctacggctcc ctcgtccgct ccatcgttgg ccaacaactc
     2341 tcgacggtag ccgcccgcac catctacgga cgccttactt cgctcttcga ccaccggcct
     2401 cccactcctg cggaactcct cgccgcggac gaggagaccc tgcgcgcctg cgggctctcc
     2461 cgccccaaaa tctcttacct ccgcgacctg gcccggcacg tgctggaaga cggcctagac
     2521 ctcccaaggc tgcgcgagtt gcccgacgac gaggtcgcct caaggctcgt cgccgtcaaa
     2581 ggcattgggc agtggagtgc tgatatgttc ctgatgttcc acctcacccg tcccgacgtt
     2641 ctgcctgtcg gggacctcgg catccgccgc gccgtcgagc gggcatacgg cctggacggc
     2701 ctccctgacg cggaaaccct gcgcgaaata gccgtcccct ggcgccccca gcgcacgctc
     2761 gcgtgcctgt acctgtgggg atctctcgac aacgtccccg gctcgggata gggcgcgtgc
     2821 cgctatcatg gcgcatgcgc gtcaggggct gctagtggcc ctctacgtcg ccctcacacc
     2881 cgctcgggcg gcgtattgcc ggcctcgacg atggcgcgac gcacgttgac aagggccagc
     2941 aagacgaatc cgacgaagaa gaagatgacc agagaggcaa gcgcactacg ataactcccg
     3001 gtaagttgca gcgtaagacc gaacagaagc gggccgagcc agctcgtccc gcgctcgctg
     3061 atctcgtaca ggctgaagta ctccgcttcc tgaccgcgcg ggatcatgtg cgagaacagc
     3121 gaccgggaga gcgcctggct gccgccgagt acgatcccaa tgaacgtcgc gagcaggtag
     3181 aactgccaga ccgcacccgc ctcgaggccg tacgctaccg caacggcccc ggtccacagg
     3241 atgaggctcg ccagcacgac ccgcttggcg ccgaaaaagc gcgcacacat gccaagcagg
     3301 agcgcgccga ggaacgcgac gaactgcacc atgaggatca cggagataat agtgctcgta
     3361 ccgaacttca gctcctcttc accgtagact gcggtgagcg taatgacggt ctggatgccg
     3421 tcgttgtaga gtaggtacgc cgcgaggaag agcagggtct gcgggtacgc cctcgaggcg
     3481 cgcagggtgc ctagcaactg gcgaaaacct gcggtgacga ccgccgcgcc ctgttcgccg
     3541 gcatgcacgg gcccccgatc tcgaagcgtt ctaagtggga tcagggtgaa cagcgcccac
     3601 catgctcctg ccgaaagcag gctgatgcgt actgcatgac cagccgagag cccgagggac
     3661 tcatgcacgg tgaacagagc gaggttggcg gcgagaagca agccgccgcc gaggtaacca
     3721 agagcccagg cccgagagga gacgcggtcg cgctcgtcgg gctcagcgat ctcgggcagg
     3781 aacgcgttgt agacgactat cgacgcacca aacgacaggt tcgcgattac aaagagcatg
     3841 ccgccgaaca ggtatgcgga accctggatc aggtaaagtc ccatcgtgga gatcgcgccg
     3901 atgtaggcga acagcgccag gagctgcttc ttgcgctgtg tccggt
//
LOCUS       DATAIR010000293         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_19062, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000293 DATAIR010000000
VERSION     DATAIR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19062"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..123
                     /gene="rrf"
                     /locus_tag="VHM69_07135"
     rRNA            7..123
                     /gene="rrf"
                     /locus_tag="VHM69_07135"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            195..581
                     /locus_tag="VHM69_07140"
     CDS             195..581
                     /locus_tag="VHM69_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX2740205.1"
                     /translation="MTLGNTDLDQEFIERQRERLESLREELMRIKDSLEEEERQIGEE
                     EGDTQLDSGDLSQDMFTRELDATVEQTAEKRLHYVERALQKIEEGSYGICDDTGEKIP
                     KGRLEAVPEAIRTVEAQERFERRPPV"
     gene            607..1326
                     /locus_tag="VHM69_07145"
     CDS             607..1326
                     /locus_tag="VHM69_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2740206.1"
                     /translation="MDNRSSGEETREPIFRSDLEGPRPEPRYEPLRPEGGPWSVIKRL
                     LAPLAVVGLLLAKFKGLALLLLKVKFLGTALTMLVSIGAYALLFPVWFAVGIVVLIWV
                     HEMGHVLQLRREGIPASAPMFIPFLGAFVAMKQMPKDALAEARVGLAGPVLGTLGGLA
                     TLGLYAATKEPLFLGLAYFNSIINLFNLAPMLPLDGGRAVGALSPLFQVLGLVFMVAL
                     FFVAPIMVLVALIGLPELWHR"
     gene            1478..2647
                     /locus_tag="VHM69_07150"
     CDS             1478..2647
                     /locus_tag="VHM69_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX2740207.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALKAAQAGKKVLVMSTDPAHSLA
                     DAFDTEIGPDPKEMATGVWAQEMDHTSMIEDSWSEIQGYMTTLFEWQGADTLAAEELA
                     MLPGMDELFGLLMVRRHNKEGRYDALILDAAPTGETLKLLSLPDHMSWYIEKIFPIQR
                     RAAKLVRPFASRAKSLPPLPQDSVFAAGQRFYEAIASVEEILTNRESASVRLVLNAEK
                     MVIAEARRAYTYLNLYDYGVDAVVVNRLLPESVSDPYFARWREAQARHMQTIEESFAP
                     IPILTARLFDREMFGLEALGALAEDVFEDAEPLEVLFRGATHDIVKDGGGYDVVFHLP
                     LVEKRNVDLSKKGAELFVKVGNYRRNILLPDALARLPAAGASFDGDLLKVRLNYE"
     gene            2640..2843
                     /locus_tag="VHM69_07155"
     CDS             2640..2843
                     /locus_tag="VHM69_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740208.1"
                     /translation="MSEERQRRGSLLGVVAYVLVPGERRVRAAGHFRKAGFEALKGVG
                     ALMRPEGSGEPKETTRRELIEIE"
BASE COUNT          529 a          852 c          959 g          524 t
ORIGIN      
        1 ttttgaaagg gtgacgatgg cggcggggat acacctcttc ccattccgaa cagagaagtt
       61 aagcccgcca gcgccgatgg tactgcgggg tagactccgt gggagagtag gtcgtcgccc
      121 attttttctg cttttttgac ccgggaccta tcttcgtatg ggtccctgcg ggcaagtgtt
      181 ctacaaggat tggagtgacg ttgggcaaca cggacctcga ccaggagttc atcgaacgac
      241 agcgggagcg gctcgagtcg ttgcgcgaag agttgatgcg tataaaggac tccttagagg
      301 aagaagagag gcagatcggc gaggaagagg gcgataccca actcgactca ggagacctca
      361 gccaggatat gttcacgcgc gagctcgacg ccaccgtcga gcagacggcg gagaagcgcc
      421 tccattacgt cgaacgcgca ctgcagaaga tcgaagaggg atcttacggt atctgcgacg
      481 acaccgggga gaagatcccg aagggtaggc tggaggccgt gcccgaggcc atcaggaccg
      541 tggaggccca ggagcgcttc gagcggcgcc cgccggtata gcgccgcctg acggtataat
      601 ccgaccatgg ataatcgttc gtcaggtgaa gagacaagag agccaatctt ccgcagcgac
      661 ctcgaagggc cgaggcccga gcccaggtac gaacccctga ggccggaagg cggcccgtgg
      721 agcgtcatca agcgtttgct cgccccgctg gccgtggtcg ggttgctgct cgcgaagttc
      781 aaggggctcg cgctccttct cctcaaggtc aagttcctcg gcacggcgct gaccatgctg
      841 gtaagtatcg gcgcctacgc cctgctcttc cctgtatggt tcgcggtggg gatagtggtc
      901 ctgatctggg ttcacgagat ggggcacgtc ctgcaactca ggcgcgaggg gatccccgct
      961 tcagccccta tgttcatccc gtttctcggg gctttcgtgg cgatgaagca gatgccgaaa
     1021 gacgccctgg ccgaggcgag ggttgggctg gcaggcccag tgctgggtac gctcggcggc
     1081 ctcgccaccc tcggcctgta cgccgctacg aaagagcccc tgttcctcgg actggcgtac
     1141 ttcaattcca tcatcaacct gttcaacctc gcgccgatgc tgccgctcga cggcggcagg
     1201 gcggttgggg cgttgtctcc gctcttccag gtgctcggcc tcgttttcat ggtggcgctg
     1261 ttcttcgttg ccccgatcat ggtgttggtc gcactgatcg gtctgcccga actctggcac
     1321 cgctagaaga cgcgcaagac gccagaaggc agggcttact acgaccttcc agcgagcaag
     1381 cgcgtggctg tcggtgtcgt ttacctgggt ctcatcctgg tcctcgggtt gctgacgggg
     1441 gcgacgttcg ttcctgaccc ggcggctctg cagtagcttg cgcaccatcc tctacactgg
     1501 caagggcgga gtcggcaaga ccagcgtggc cgcggcgacc gccctgaagg ccgcgcaggc
     1561 cgggaagaag gtcctcgtga tgagcacgga ccctgcgcat tcgctcgctg acgccttcga
     1621 cacggagatc ggacccgacc cgaaggagat ggcaaccggc gtctgggcac aggagatgga
     1681 ccacacctcc atgatcgaag atagctggag cgagatccag ggctacatga ccaccctttt
     1741 cgagtggcag ggcgccgaca ccctggctgc tgaggaactg gcgatgctgc ccgggatgga
     1801 tgagctcttc ggactcctga tggtcaggcg tcacaacaag gagggtcgat acgatgcgtt
     1861 gatcctcgac gcggcgccca ccggcgagac gctcaagctg ctgagcctcc ccgaccacat
     1921 gagctggtac atcgagaaga tcttccctat acagcgccgc gccgcgaagc tcgtgaggcc
     1981 gttcgcgagc cgggcgaagt ccttgcctcc gctaccgcag gacagcgtct tcgcggcggg
     2041 ccagaggttc tacgaggcca ttgcgagcgt cgaggagatc ctgacgaacc gcgaaagcgc
     2101 gtctgtgcgc ctggtgctca atgcggagaa gatggtcatc gctgaggctc gccgcgccta
     2161 cacgtacctg aacctctacg actacggcgt ggacgctgtg gttgtgaacc ggctgcttcc
     2221 cgagtccgtc agcgacccgt acttcgcccg gtggcgcgag gcgcaggcga ggcacatgca
     2281 gaccatcgag gagtcgttcg cccccatccc gatcctgacc gcgcggctct tcgaccgcga
     2341 aatgttcggg ctggaggcgc tcggcgccct ggccgaggat gtcttcgagg acgctgaacc
     2401 tctggaagtt ctgttccggg gcgccacgca tgacatagtg aaggacggcg gcggatacga
     2461 tgtcgtcttc cacctcccgc tggtggagaa acggaacgtg gacctctcga agaaaggcgc
     2521 ggagttgttc gtaaaggtgg gcaattatcg gcgcaacatc ctgctccccg atgccctcgc
     2581 ccgtcttccg gcggccgggg cgagcttcga cggagatctg ctgaaggtga ggttgaatta
     2641 tgagtgagga gaggcaacgc agaggctcgc tgctcggcgt ggtcgcctac gtccttgtac
     2701 cgggcgagcg gcgggtgcgg gccgcggggc acttccggaa agcggggttc gaggcgctga
     2761 aaggcgtcgg ggcgctgatg cgccccgagg gatcgggcga accgaaggaa acgacgcggc
     2821 gcgagcttat cgagatcgag taggattgtc gctgtcgacc tgac
//
LOCUS       DATAIR010000294         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_111480, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000294 DATAIR010000000
VERSION     DATAIR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111480"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VHM69_07160"
     CDS             complement(<1..303)
                     /locus_tag="VHM69_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740209.1"
                     /translation="MGLKVGIYGGSGYAGGELVRLLAGHPEVGAMSVASRGYAGVGIS
                     EVYPQLAVDGSYMAPEEIEAGGLDVAFVAYGHGESAGAVKELLRNGTHLVIDLSADF"
     gene            486..1619
                     /gene="argJ"
                     /locus_tag="VHM69_07165"
     CDS             486..1619
                     /gene="argJ"
                     /locus_tag="VHM69_07165"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX2740210.1"
                     /translation="MRNSGRRDLGLLFSELPCETSAVFTRNAVKGAPLHVTREAVDTG
                     GVRAVVANSGNANAATGERGVEDARVMQALAAEALGIGTGEVAVASTGVIGVHLPMEK
                     IAGGIQAASGELGESGAGFAESILTTDTRAKEAAVRVEIGGRTVTVGGTAKGSGMIHP
                     NMGTMLAFLTTDAAVEKDCLQETLSRVTDSTFNRVTVDGDTSPSDMALLMANGAAGNE
                     PLTLDSPEYPAFAEAVESAARVLAKEIARDGEGATRLVEVVVEGAASEESAAALAKSV
                     VGSSLVKAAVFGEDANWGRVLTAMGYSGVAFDPDGVELWFGPVKVFSGGEPVPHEEAE
                     ANATLAAGEVRITARLGEGDASATAWGCDLSYEYVRINGSYRT"
     gene            1616..2383
                     /gene="argB"
                     /locus_tag="VHM69_07170"
     CDS             1616..2383
                     /gene="argB"
                     /locus_tag="VHM69_07170"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2740211.1"
                     /translation="MKPVVIKVGGGTLAGGALEDLPGLLLEGHRVAVVHGGGVQLTRM
                     LDALGIPTRFHAGLRVTDEKALEVAEIVLAGGVNKTLARGLNAIGVQAAGISGTDGPT
                     LLVEPVPDLGRVGKVVRVEPHLVKTLWAGGFVPVVAPLGLGTDGAYNVNADMAAAALA
                     VGLGAGNLFLLTDVDGLLEGGETVQTIAPEECEGYVRSGLAAGGMVPKLRAAAEAARG
                     GVEARIINGNKKGALAAAIAGEQVGTLISEGVAACKQ"
     gene            2371..>3174
                     /locus_tag="VHM69_07175"
     CDS             2371..>3174
                     /locus_tag="VHM69_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2740212.1"
                     /translation="MQAVMETYKRLGIAPTSGKGSWLFDEEGNRYLDFIGGIATNSLG
                     HGHPALVEAIQDQAEKLIHCSNLYEIPLQAEVAGMLTGATDFDRVFFCNSGAESVEAA
                     IKLSRKYAHENFGPYKHEILTFSKSFHGRTYGGLTATAQPALQEGFGPMLPGMVHAPF
                     GDLEAAKAMVGPQTAAVLVEPIQGEGGVNEPPEGFLEGLRELCDEYGALLIFDEVQTG
                     AGRTGHLYAYQGTGVVPDVLTSAKGLGGGFPVGAMMVKVEYAALGPGNHG"
BASE COUNT          577 a          964 c         1106 g          527 t
ORIGIN      
        1 gaagtccgcg gagaggtcga tgaccaggtg agtcccgttc ctgagtaact cctttaccgc
       61 cccggcgctc tcaccgtgcc cgtacgccac gaacgccacg tcgagcccac cggcttcgat
      121 ctcctccgga gccatgtacg acccatcgac ggcgagctgc ggatagacct cactgatacc
      181 aacccccgca tacccgcgcg acgcaacgct catcgcccca acttccggat gccccgcaag
      241 cagccgcacg agctcgcccc ccgcgtaccc actcccccca tagatgccta ctttcagtcc
      301 catgcagccc attgtagaac cttcggatat ttattcaagt ttctgcatat tttggttgac
      361 gccctcgcac gcgggtgcga caatagcgcc ccgtgatgaa taaagattca acaatcggaa
      421 taaaatctct ctccggtggt gcggcgtctc cgtctggatt catttcggct ggggtcgtat
      481 gcggggtgcg gaactctggg aggcgggatc tcgggctcct cttctccgag cttccttgtg
      541 agacgtcggc cgtgttcacc cgcaatgcgg tcaagggcgc gccgcttcac gtgacgcggg
      601 aggcggtgga taccggtggc gtgcgtgcgg tcgttgcgaa cagcggcaac gccaacgcgg
      661 cgacggggga gcgcggcgtc gaggacgccc gtgtcatgca ggcgctcgct gccgaggcgc
      721 tgggaatcgg gactggtgag gtagcggtag cctcgaccgg tgtgatcggg gtccatctgc
      781 cgatggagaa gatcgcgggc ggaatacagg cggcgtccgg cgagctgggt gagagcggcg
      841 cgggcttcgc cgagtccatc ctcaccaccg atacccgtgc gaaagaagcg gctgtgcgag
      901 tggagatcgg tggcaggacc gtcaccgttg gcggtacggc caagggtagc ggcatgatcc
      961 accctaacat gggcaccatg ctcgccttcc tgaccaccga cgccgcagtc gagaaggact
     1021 gtttgcagga gacactgagc cgtgtgacgg acagcacgtt caaccgcgtc accgtggacg
     1081 gcgacacctc gccgagcgac atggcccttc tcatggcgaa tggcgcggct ggtaatgagc
     1141 cgctgacact cgactcgcca gagtaccccg ccttcgcgga ggccgtcgag agcgcggcgc
     1201 gagtactggc caaggagatc gcacgcgacg gcgagggggc aacccggctc gtcgaggtcg
     1261 tcgtcgaggg agcggcgagt gaggagtccg ccgctgcgct ggcgaaaagc gtcgtgggga
     1321 gcagcctcgt caaggccgcg gtcttcggcg aggacgccaa ctgggggcgg gtgctcacgg
     1381 cgatgggtta ctccggagtg gctttcgatc ccgacggagt agaactgtgg ttcggtcccg
     1441 tcaaggtctt ctctggtgga gagccagtgc cacatgagga ggccgaagca aatgcgacgc
     1501 tcgccgcggg ggaagtcagg atcacggcgc gtttgggcga gggtgatgct tcggcgactg
     1561 catggggctg cgacctgagc tacgagtacg tcaggatcaa cgggagttac cggacgtgaa
     1621 gcctgtggtg atcaaagtcg gcggcggcac cctggcggga ggggcgctcg aggatctgcc
     1681 tggtctcctg ttggagggtc acagggtcgc cgtggtgcac ggtggcggcg tccagctcac
     1741 caggatgctc gacgcgctcg ggatccccac ccggtttcac gcggggctcc gcgtgacgga
     1801 cgagaaggcg ttggaagtcg ccgagatagt gctcgccggg ggcgtgaaca agaccctcgc
     1861 gcgcgggctg aacgctatcg gggtgcaggc ggccggtatc tctgggaccg acgggccgac
     1921 gttgctcgtc gagcccgtcc ccgatctcgg ccgggtaggg aaggtcgtgc gggtggagcc
     1981 acatctcgtc aaaaccttgt gggccggcgg gttcgtcccc gtcgtcgcgc cacttgggct
     2041 cggcacggat ggcgcgtaca acgtcaacgc ggacatggcc gcggcggcgc tcgccgtcgg
     2101 gctcggggcg ggcaacctgt ttcttctcac cgatgtggac gggctactcg aaggcggcga
     2161 aaccgtgcaa acaatagccc cggaagagtg tgagggctac gtcaggagcg ggctcgccgc
     2221 tggcgggatg gtgcccaagt tgcgggccgc ggctgaagcc gcgcgcggcg gggtcgaggc
     2281 aaggatcatc aacggcaaca agaaaggcgc gctcgcggcg gccatcgcag gcgagcaggt
     2341 tggaactctc atatccgaag gagtcgcggc atgcaagcag taatggagac gtacaaacgc
     2401 ctcgggatag cgccaacgag cggaaagggt agctggctct tcgacgaaga aggcaaccgc
     2461 tatctggact ttataggtgg gatcgcgact aactctttgg gtcacggtca tcccgcgctc
     2521 gtcgaggcga tccaggatca ggccgaaaag ctcatccact gctcgaacct ttatgagata
     2581 ccgctccagg ctgaggtcgc agggatgctg acgggcgcga cggacttcga cagggtcttc
     2641 ttctgcaact caggagccga gagcgtggag gcggcgatca aactctcgcg caagtacgcc
     2701 cacgagaatt tcggtccata caagcacgag atcctgacgt tctcgaagtc cttccacggc
     2761 cgcacctacg gcgggctcac agcgacggcg cagccagcgc tccaggaagg cttcggcccg
     2821 atgctgcccg gaatggtcca tgctccattc ggcgacctcg aagcggcgaa ggcaatggtg
     2881 ggcccgcaga cggcggccgt gctcgtcgag cccatccagg gcgagggcgg tgtgaacgag
     2941 ccgcccgaag gtttcctgga agggctgcgc gagctgtgcg acgaatacgg tgcgcttctc
     3001 atcttcgacg aggttcagac gggtgccgga cgcacgggtc acctgtacgc ttaccaggga
     3061 acgggcgtcg tccccgatgt gctcacgagc gccaagggcc tcggcggcgg cttccccgtc
     3121 ggggcgatga tggtcaaggt agagtacgca gcgctcggac cgggcaacca cggc
//
LOCUS       DATAIR010000295         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_296458, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000295 DATAIR010000000
VERSION     DATAIR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296458"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VHM69_07180"
     CDS             complement(<1..677)
                     /locus_tag="VHM69_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2740213.1"
                     /translation="MRILLTGATGLLGGELLKLLLAEGHEIRCLLRRDSPNASRLDGV
                     ETFRGDAAEEEAVYRALGGADALLHVAGIEYSPGVLAASRRAGVERVVVVGSTSVHSA
                     YAFRAGPKLRMEELVRGSGLAWTIVRPSMIYGSERDRNVHRLLRFLDRSPVFPIFGPG
                     TNLWQPVFHEDCARRTRSPETPIVRTPQLRPAWSRTPCVSRHGENGGRGPGQESTHRQ
                     AADRTSA"
     gene            complement(696..1124)
                     /locus_tag="VHM69_07185"
     CDS             complement(696..1124)
                     /locus_tag="VHM69_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740214.1"
                     /translation="MRLRESPAISVLLSLALCILVAGCGGGQSGSGGDEQGQEQKGEA
                     AKKEPLQQKIAIGTIRSFKEDKRRISLRPAANAQSEKPLGFKVRKNASITLGGKKVEL
                     TDVKEGQQAQIEYVVQNNVNRAVAVHLFEVQDSGGGEESG"
     gene            complement(1235..>1715)
                     /locus_tag="VHM69_07190"
     CDS             complement(1235..>1715)
                     /locus_tag="VHM69_07190"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX2740215.1"
                     /translation="RLRKKSTPYEEAAGVPLVIRGPGVPAGEVRDQLVITNDFAPTFA
                     DWAGLDTPRFVDGRSLAPLLEPDPPASSAWRTAILNERNQKTPSHIPDYEAVRTRTHT
                     YVQWETGERELYDLRRDPYELDNIQETADPTLVARLKTRLKLLQNCAGTTCRAAEGP"
BASE COUNT          272 a          548 c          557 g          338 t
ORIGIN      
        1 tgcgcactgg ttcgatcggc agcttgacga tgcgtggact cctgcccagg gccgcggccg
       61 ccgttttcac catgtcgaga tacgcaaggg gttcggctcc aggcaggtcg tagctgcggt
      121 gtacggacga tgggcgtttc agggcttcga gtacgccgcg cgcagtcttc gtgaaagacg
      181 ggctgccaga ggttcgtacc tggaccgaag atcgggaaga cgggcgagcg gtcgaggaaa
      241 cgcagcaggc ggtgcacgtt cctgtcccgc tcggagccgt agatcatgga cgggcgcact
      301 atcgtccacg cgagcccgct gccgcgtaca agttcctcca tcctgagctt cggaccggcg
      361 cggaaagcgt aggccgagtg cacgctcgtg ctgcctacca cgacgactcg ttcgacaccg
      421 gcgcggcggg acgccgccag gacacctgga gagtattcga tccctgcgac gtgcaggagg
      481 gcgtcggcgc cccccagtgc gcggtagaca gcttcctcct cggcggcgtc cccacggaag
      541 gtttcgaccc cgtcgagccg ggaggcgttg gggctgtccc gacggaggag gcagcgtatc
      601 tcgtgccctt cggccagcaa gagcttcagg agctcgcccc cgagcagccc cgtggcgccc
      661 gtgagaagta tcctcatgcg gtcgccgcgg gggacctagc ccgactcctc gcctcccccg
      721 gagtcctgta cctcgaacag gtgcacggcg acggccctgt tcacgttgtt ctggacgacg
      781 tactcgatct gggcctgctg gccctccttc acgtcagtga gctcgacctt cttcccgcca
      841 agtgtgatac tggcgttctt cctgaccttg aagccgagcg gtttctcgct ctgggcgttt
      901 gcggcgggcc gcagggagat cctgcgcttg tcctctttga aggaccttat cgtcccgatc
      961 gctatcttct gctggagcgg ctccttcttg gcggcctcgc ctttctgttc ttgaccttgc
     1021 tcgtcaccgc cggaaccaga ttggcctccc ccgcaaccag ccaccaggat gcagagggcg
     1081 agcgagagga gtacggaaat ggccgggctt tctctgagac gcatgggagg atctccttag
     1141 actgtcaacc gagacgatta gcccggtcaa actaacagaa atgtctacgg attatcaacc
     1201 ggtcccggcg cccaccatga tgcctccagg cggtctaagg tccctcggcc gccctgcacg
     1261 tcgttcccgc gcagttctgg agcaatttca ggcgcgtctt caaacgcgct accagggtgg
     1321 gatcggcggt ctcctggata ttgtccagct cgtagggatc ccggcgcagg tcgtagagct
     1381 ctcgttctcc ggtctcccat tgcacgtagg tgtgtgtccg ggtacggacg gcctcgtaat
     1441 cggggatgtg ggatggtgtc ttctgattgc gctcgttgag gatagccgtc ctccaggcgg
     1501 agcttgccgg cgggtcgggc tcaagaagcg gcgcgaggct ccggccgtcc acaaatctgg
     1561 gggtgtctag gccggcccaa tcggcgaacg taggcgcgaa gtcgttggtg atcaccagct
     1621 ggtctctgac ctcgccagcc ggtacaccgg gtccgcggat gacgagcggc accccggccg
     1681 cctcctcgta aggcgtggat ttctttcgca gccga
//
LOCUS       DATAIR010000296         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_54780, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000296 DATAIR010000000
VERSION     DATAIR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54780"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VHM69_07195"
     CDS             <1..1486
                     /locus_tag="VHM69_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HEX2740216.1"
                     /translation="YGERRYTYAELEERVNRLSSRLRDSGLEKGDRVAFLCFNTPPQL
                     EAHFAVPAAGLVLVAINTRLGKDEVTYIVEHSGAKMVFVDAELEKLLAEVDEEVERIR
                     VDDTGEEGDPYEDYIAEGSPEPVESVLEDEEETISINYTSGTTGRPKGVMYTHRGAYL
                     SALGNVIEIGMGYDTRYLWTLPMFHCNGWTYPWAVTAIAGTHVCLRKVESPRIWELFE
                     SEGITHYCAAPTVQIGIVNEDASHPLDTPVIAAIAGAPPSPTLLSGLLELNISPMHIY
                     GLTETYGPMTTSGVHPEWEDLDMEERARLMARQGQGYCTADLVRVVDENMNDVERDGE
                     AMGEIVMHGNMVAKGYFENEEATEEAFEGGWYHSGDVAVWHPDGYIEIRDRNKDIIIS
                     GGENISTIEVEQVVDQHPAVMEAAVVAMPDEKWGERPKAFVTLKKGQEATEQEIIDFC
                     KEHIARFKAPAAVEFTELPKTSTGKVQKFVLRDKEWAGQAKKVN"
     gene            1647..2438
                     /locus_tag="VHM69_07200"
     CDS             1647..2438
                     /locus_tag="VHM69_07200"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX2740217.1"
                     /translation="MIEAEKTYEHIHFEQDGRVAYVTMNRPDKRNALSLDHMRELTSC
                     FKEIGDSREVSVVILRGEGPAFCAGHDLSEMVGRDPEFYRHLFDVCCELMETIQAIPQ
                     PVIAQVHGVATAAGCQLAATCDLVVASEEARFATPGVRIGLFCSTPMVALSRAVGQKK
                     SMEMLLTGDFISAKEALAEGLVNKVVAADDLESETRALAEKIVEASPLVVGVGKQAFY
                     RQLEMPVEQAYAYTREVMSFNATFADAQEGICAFLEKRKPEWKGR"
     gene            2456..2779
                     /locus_tag="VHM69_07205"
     CDS             2456..2779
                     /locus_tag="VHM69_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX2740218.1"
                     /translation="MTGTIARPVEKKEQKTEGLPPYNVVLLDDDDHSYEYVILMLKRI
                     FGHPVEKGYEMAREVDATGRVIVATTNLEQAELKRDQIQSFGPDPLIPRCKGSMSATV
                     EPAQA"
     gene            complement(2794..4092)
                     /locus_tag="VHM69_07210"
     CDS             complement(2794..4092)
                     /locus_tag="VHM69_07210"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740219.1"
                     /translation="MEDTRRTTQDAYPADLALFVRERWDEGKGGPLPDTDTLETLIST
                     CYQASLLREEERAVTFRVILCEPDRLPPREGPPGGIHRLEFPELRQFDLQEMRRLSPA
                     ADYYRSLIGILWDEEEGLRIWGLVHSGPRWLRADQGGRNAPPPLPPVPILRVRGPGRI
                     AVDVGSEALGKLDEGRLSDVSMDVFTSRWFPATFAPVRGELAEIHARLREQARKKGEV
                     WARLDNDVTRVIGQHTIKRIISAVRGSHHGGTLVIVPPELAEDILQDRYVSLKYKFID
                     GEPRRRFRTLIVDVMNTLARSYAGTPARDHVGWDDYEASSDGNLSELDEAIFEVAHLI
                     TGLTAVDGAVVMTQRFELLGFGGEISGELPAVETVAKALDVEADEIVEEPAESFGTRH
                     RSAYRLCNELPDVIAVVISQDGNIRFVKCKAGAVTYWNQI"
     gene            complement(4103..>5229)
                     /locus_tag="VHM69_07215"
     CDS             complement(4103..>5229)
                     /locus_tag="VHM69_07215"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2740220.1"
                     /translation="LLAVLLALACASCDRTNEERGENGNDFSPITTGGAETTGAVATR
                     VERVEVSTLVTNLDVPWSFAFLPDGDALVTERDSGRLLRISPSGNVREIQTLPESGSG
                     EGGLLGVAISPDYEEDHYVYAYYTTEVDNRVVRFRLGERPQPILTGIPANTFHDGGRI
                     KFGPDGMLYVSTGDAGDSASSQDRNSLGGKILRIEPDGSVPSGNPFPGNPVYTYGHRN
                     VEGLAWDAEGQLYASEFGQNAWDEVNRIEPGQNYGWPEVEGRGGEDLGYVDPITVWPT
                     SEASPSGAEIMLGGAIPQWEGDLFVTALRGERLWRLELNGRGEVVEREKLLDHEVGRV
                     RDVAQAPDGSLWVSTSNHDTYGSPVSEQDDRILRLAPAGE"
BASE COUNT         1011 a         1654 c         1649 g          915 t
ORIGIN      
        1 ctacggcgag cgccgctaca cctacgcgga gcttgaggag cgggtcaacc gtctctcctc
       61 gcgcctgcgc gactccgggc tggagaaagg tgaccgggtg gcgttcctgt gcttcaacac
      121 cccgccccaa ctggaggcgc acttcgccgt gccggccgca gggctcgtgc tcgtcgccat
      181 aaacacccgc ctcggcaaag acgaggtcac ctacatcgtc gagcactcag gcgcgaagat
      241 ggtcttcgtc gacgccgagc tagagaagct gctcgctgag gttgacgaag aggtggagag
      301 aatacgcgtc gacgatacgg gtgaggaggg cgacccgtac gaggactaca tcgccgaagg
      361 gtcgcccgag cccgtggaga gcgtgctgga agacgaggag gagacgatct ccataaacta
      421 cacctcgggc accacgggac gtcccaaagg cgtgatgtac acccaccgcg gggcgtacct
      481 cagcgccctc gggaacgtca tcgagatcgg gatggggtac gacacccgct acctgtggac
      541 gctcccgatg ttccactgca acggctggac ctacccgtgg gccgtaacgg ccatcgccgg
      601 gacccacgtc tgcctgagga aagtcgagtc gccccgcatc tgggagctct tcgagtccga
      661 gggcataacg cactactgcg ccgccccgac ggtccagatc gggatagtca acgaggacgc
      721 ctcccacccg ctcgacacgc ccgtgatagc agccatcgcg ggcgctcccc catcccccac
      781 actcctgagt gggctcctcg agttgaacat cagcccgatg cacatctacg gtctcacgga
      841 gacgtacggt ccgatgacga cgagcggcgt ccacccagaa tgggaggacc tggacatgga
      901 ggagcgggcg cggctcatgg cgcgccaggg acagggatac tgcacggcgg acctcgtgcg
      961 cgtggtcgac gagaacatga acgacgtcga gcgcgacggg gaggccatgg gtgagatcgt
     1021 gatgcacggc aacatggtcg ccaaaggcta cttcgagaac gaggaggcca ccgaggaggc
     1081 gttcgaaggc ggctggtacc actcgggcga cgtcgccgtg tggcatcccg acgggtacat
     1141 cgagatccgg gaccgaaaca aggacatcat catctccggc ggcgagaaca tctcgaccat
     1201 cgaggtggag caggtagtcg accagcatcc cgccgtgatg gaggccgccg tagtcgcgat
     1261 gcccgacgag aagtggggcg aacgtcccaa agctttcgta accctgaaga agggtcaaga
     1321 ggccacggag caggagatca ttgacttctg caaagagcac atcgcccgct tcaaggcccc
     1381 cgccgccgtc gagttcacag aattaccaaa gacatcgacg ggcaaggtgc agaagttcgt
     1441 cctgcgcgac aaggagtggg caggccaagc gaagaaggtg aattagaggt ttcaggcatc
     1501 aggtttcagg tctttgctcc ggcggatctc gtagcgcaaa accgtccgcc gtgcgaagcc
     1561 gatccactct ccgcagccgg atatacgaca tcgaggccct gatgcctgta acctgatgcc
     1621 tgaagcctat cttcaaggag gcgaacatga ttgaagccga gaagacctac gagcacatcc
     1681 acttcgagca agacggacgg gtcgcctacg tgacgatgaa ccgtcctgac aagcgcaacg
     1741 ccctctcgct cgaccacatg agggagctga cctcctgctt caaggagatt ggagatagca
     1801 gggaagtctc ggtcgtcatt ctgcgcggcg agggtccggc gttctgcgca gggcacgacc
     1861 tttccgagat ggtcgggcgc gaccccgagt tctaccgcca tctcttcgac gtgtgttgcg
     1921 agttgatgga gacgatccag gcgatcccgc agcctgtgat cgctcaggtc cacggcgtcg
     1981 ccaccgcagc aggctgccag ctcgccgcga cctgcgacct cgtcgtggct tctgaggaag
     2041 cccgcttcgc cacgcctggc gtgcgcatcg gactcttctg ctccactccc atggtcgccc
     2101 tctcccgcgc cgtcggccag aagaagtcga tggagatgct cctcacgggc gacttcatct
     2161 cagccaagga agccctcgcg gaaggcctgg tgaacaaggt cgtcgcggca gatgacctgg
     2221 agagtgaaac gcgggcgctt gcggagaaga tagtagaggc gagcccactc gtcgtcgggg
     2281 ttggcaagca ggctttctac cgccagctcg agatgccagt cgagcaggcg tatgcctaca
     2341 ccagggaggt catgtccttc aacgcgacct tcgcagacgc ccaggagggc atttgcgcct
     2401 tcctggagaa gcgcaagcca gagtggaagg gccgctgagt cagtagaggt ggagaatgac
     2461 cggcacgatc gcgcgtccag tcgaaaagaa agagcagaaa acggaggggc ttccgcctta
     2521 taacgtggtc ttgctagacg atgacgatca cagctacgag tatgtgatcc tcatgctcaa
     2581 gaggatcttc ggccatcctg tcgagaaggg ttacgagatg gcgcgtgagg tcgacgcgac
     2641 ggggcgggtg atcgtcgcga ccacgaacct ggagcaggcc gagttgaagc gcgaccagat
     2701 ccagagcttc ggtccagacc cgctcatacc acgttgcaag ggctctatgt cagcaaccgt
     2761 cgagcccgct caggcctgat cgacggagcg gagctatatc tgattccagt aggtgacagc
     2821 gccggccttg cacttaacga agcgtatgtt gccgtcctgc gagatgacga cggcgatgac
     2881 gtcgggaagc tcgttgcaga ggcggtatgc ggagcgatgg cgggttccga agctttccgc
     2941 cggctcttcg acgatttcgt cagcctccac gtcgagggcc ttcgcgaccg tctcaacggc
     3001 tggtagctcg ccggagatct cacctccgaa gccgagcaac tcgaagcgct gggtcatcac
     3061 gaccgcgccg tccacggcgg tgaggccagt gatcaggtgc gccacctcga agattgcctc
     3121 gtccagctcg gacaggttcc cgtcgctgct cgcttcgtag tcgtcccacc cgacatggtc
     3181 tctggctggg gtgcctgcgt aggaccgggc caacgtgttc atcacgtcga caatgagggt
     3241 gcggaagcgc ctgcgcggct caccgtcgat gaacttgtat ttcagggaca cgtagcggtc
     3301 ttgaaggatg tcctcggcca gctccggcgg tacgataacc agggtcccgc cgtggtgaga
     3361 gccacggacc gcggagatga tgcgctttat cgtatgctgc cctataacgc gggtaacgtc
     3421 gttgtcgagg cgggcccaga cctccccttt cttccgcgcc tgctcgcgca gccgtgcgtg
     3481 gatctccgcg agttcgcccc gaacgggagc gaaagtagcg gggaaccagc gtgaggtgaa
     3541 cacgtccatc gagacgtcag agagcctgcc ctcgtccagc ttgccgaggg cttcggaacc
     3601 aacgtcgacc gcaatgcgtc cgggtccgcg cacgcgcagg atcgggaccg ggggcagcgg
     3661 aggcggggca ttcctcccgc cctggtccgc cctcaaccac ctcggccccg aatggacgag
     3721 accccagatc cgcaatccct cttcctcatc ccagaggatc ccgatgagcg agcggtagta
     3781 gtcggcggcg ggcgagagcc gcctcatttc ctggaggtcg aactgtctga gttcggggaa
     3841 ctccagtcga tgtatcccgc cgggaggccc ttcgcgcggc ggcagacggt caggctcgca
     3901 gaggatcacc ctgaacgtca cggcacgttc ctcctcgcgc agcaggctcg cctggtagca
     3961 ggtcgagatc agggtttcca gtgtatcggt gtcgggtaga gggccacctt taccctcgtc
     4021 ccaccgttca cggacgaaaa gagcgaggtc cgcagggtac gcatcctgtg tggttctcct
     4081 cgtgtcttcc acggaaccca ttctattccc ccgccggtgc caggcgcagg atgcggtcgt
     4141 cctgttcgct caccgggctt ccgtaggtat cgtggttgga ggtcgatacc cacagagacc
     4201 cgtcgggggc ctgcgccacg tcccgcacgc ggccgacctc atggtcaagt aacttctccc
     4261 tctccacgac ctcgccccgg ccgttcagtt ccagccgcca gagccgctct cctctcagtg
     4321 ccgtcacgaa gaggtcgcct tcccactgcg ggatggcccc gcctaacatg atctcagccc
     4381 cgctcgggga ggcttctgaa gtcggccaga cggtgatggg atcgacatac ccgaggtcct
     4441 cgccacccct gccctcgacc tctggccagc cgtagttctg gccgggctcg atcctgttga
     4501 cctcgtccca ggcattctgc ccgaactcgc tggcatatag ctgtccctcc gcatcccagg
     4561 caagtccctc cacgttgcga tggccgtagg tgtaaacggg gttgccgggg aaagggttgc
     4621 ccgaaggaac ggacccgtcg ggttctatcc gtaggatctt acctcccaaa gagttcctgt
     4681 cctgggagct tgcagaatct ccggcgtcgc ccgtactcac gtagagcatc ccgtcgggtc
     4741 cgaactttat cctacccccg tcgtgaaagg tattggcggg gatgccggtc agtatgggct
     4801 gcggcctctc gccgagccgg aagcggacca cccggttgtc gacctcggtc gtgtaatagg
     4861 cgtagacgta gtggtcctct tcatagtcgg gggagatggc gacgccgagc aacccgccct
     4921 cgcccgagcc gctctccggc agggtctgga tctcccgaac gttgcctgat ggactaatcc
     4981 tgaggagcct tcccgagtcg cgctcggtga cgagagcatc cccgtcaggc aaaaaagcga
     5041 aagaccaggg cacatcgagg ttcgtgacga gtgtactcac ctcgacccgc tccacgcgtg
     5101 tcgcaacagc acccgtggtt tcggctcctc cggtagtgat cgggctgaag tcgttaccgt
     5161 tctcccctct ctcttcgttg gttctgtcgc acgacgcgca cgcgagagcg agcaggacgg
     5221 cgagcagca
//
LOCUS       DATAIR010000297         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_296496, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000297 DATAIR010000000
VERSION     DATAIR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296496"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..331)
                     /locus_tag="VHM69_07220"
     CDS             complement(155..331)
                     /locus_tag="VHM69_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740221.1"
                     /translation="MHRRLSILLAVALMLALMLASSGMASAAALANGKGGEMASTSAA
                     FGIATAISNTIKHG"
     gene            complement(406..600)
                     /locus_tag="VHM69_07225"
     CDS             complement(406..600)
                     /locus_tag="VHM69_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740222.1"
                     /translation="MTVNIVGAARDRGLFASPIGDEETFLLLLAAKDARYIREQEEAA
                     IDEEDFWTQAEEANAIDPIG"
     gene            complement(615..806)
                     /locus_tag="VHM69_07230"
     CDS             complement(615..806)
                     /locus_tag="VHM69_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740223.1"
                     /translation="MIGNLLSSEEANAALVVVLSATVMALVAKGNGPLSPERLLARVL
                     PDPVARRGPRIEQSSRVAA"
     gene            1034..2473
                     /locus_tag="VHM69_07235"
     CDS             1034..2473
                     /locus_tag="VHM69_07235"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX2740224.1"
                     /translation="MGHSGRTIVVSNRGPVTFSRGESGEREHSRGAGGLVTALNAVLR
                     RAENAVWIASAQSEEDVAVSKEPAPYEVEDLRVRLVEHDPESYDLMYNHLANPLLWFV
                     HHGLYNLPYSPELGEDTRRAWEEGYVPVNRNFAEAVIETAAGDDAPVVFLHDYQLYMA
                     PLFVRERLGEDALLSLFVHIPWPEPDLWRVLPRYIREGVLESLLSADIVAFHTHRYAH
                     AFAETAAAVLGAEVEEGVIHYRGREVWVRAYPISIDPEEFEDLALSEAVLEQERFVKD
                     LPGKLLLRVDRTDLSKNVVRGFVAYGRMLERHPEMKGEVTFLAQLQPSRTDIPEYAAY
                     MEAVGRTAEEVNQKHGTGSWRPIELFMEDHFPRSVAAYKNFDVLLVNAVRDGMNLVAK
                     ESAVINEKNGVLVLSENAGAHEELGEYALTVNPYDIDEQADAIHEALTMPEEERERRS
                     RALHDTVMSNTIEHWVEAQMKDIEAYRER"
     gene            2479..2934
                     /locus_tag="VHM69_07240"
     CDS             2479..2934
                     /locus_tag="VHM69_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740225.1"
                     /translation="MRIFLMAAAVVVGLANATLYSLIGNNTFDDLFEWQTDPWSLYVM
                     YALCAVFVGLLISGGLLKFAERDLQDRFFARYGVMVLAICIGGAMLAIFLTTATFMFD
                     ENAYVPRRVSETFYTLALVTIPGAMLGAIEGVVLAFPLAWVLGLFRKRS"
     gene            complement(2931..3731)
                     /gene="otsB"
                     /locus_tag="VHM69_07245"
     CDS             complement(2931..3731)
                     /gene="otsB"
                     /locus_tag="VHM69_07245"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX2740226.1"
                     /translation="MNSHGREAWERLRTWGEEPGSVAVLTDIDGTLAPIVPTPDMSEV
                     SEELRDLLRRLSEKVLLVAGISGRKTEDAYELIGLEDVLYFGNHGFEILRDGEVEVIP
                     DALPYLDRVQELERMAREELTPEGALVEEKGITASVHYRNAPREVGERSVEFVKREGE
                     RLGLRITVGRDVVEARPPIKADKGTAVRTLVEEYRPDMAMFIGDDTTDLDAFRELEKL
                     REEGALREILRVGVASGEGPPEIETEADIVVDGVDGVAGVLRALLREG"
     gene            complement(3772..3972)
                     /locus_tag="VHM69_07250"
     CDS             complement(3772..3972)
                     /locus_tag="VHM69_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEX2740227.1"
                     /translation="MATWTNKHWGAVIGALVVFSMFLIGFEETLVVVLFGIIGYFIGK
                     YLDGEIDTEDIRARAQGRENGM"
     gene            complement(3990..>4631)
                     /locus_tag="VHM69_07255"
     CDS             complement(3990..>4631)
                     /locus_tag="VHM69_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740228.1"
                     /translation="LREGVAGEQTQRQARRSGPETDENEGAVNAFNRLILLILSLLLL
                     AVPIVLLLITYGVLPADVVNQYTGYRVALEALEGFSASSLTITTRVIIAVVGALVALI
                     ALLLLLRELTFGRRVTRSTVMDDTPGKETVITANAVKTLAESAAREAGAESPSASLAS
                     DNDSYIVFAGIQVPSSENYSELATRARENIHSVLHDQSVPVKEVEVTVRGTAS"
BASE COUNT          843 a         1498 c         1385 g          905 t
ORIGIN      
        1 cttattcagg ggtttagcat atcttcggaa cggtctccga gagactttag ggactcgtat
       61 gcgttgatag ggggagaaag taaagagaac cggggctccg tgtgaggtcg ccccggctag
      121 cgtgattcta agagttgcga ctcaacatta gatgtcaccc gtgctttatg gtgttggaga
      181 tggccgtcgc tataccgaac gccgcgctgg tgctggccat ctcgccgccc tttccgttgg
      241 cgagagcggc ggccgaagcc atgccggacg aggccagcat cagcgccagc atcagggcca
      301 cagccaacag tatcgatagt cttctgtgca tcgcgtttcc tcctttgacc tggtgggagg
      361 tatgggtcgc tgttctccgc ttgatcgaga gcagccgaac ctcatttacc cgatgggatc
      421 gatggcgttt gcttcttcgg cttgcgtcca gaaatcttct tcgtctatgg ccgcctcctc
      481 ttgctctcgg atgtacctcg cgtccttggc ggcgagcaag agcaagaacg tctcctcgtc
      541 gcctatgggg gacgcgaaca gacccctgtc cctcgctgcg ccgacaatgt tgaccgtcat
      601 ggtctcctcc ttcctcaagc cgcgacccgc gacgactgct cgatcctcgg cccacgcctg
      661 gcgacgggat ccggcaagac ccttgccagg agccgctcgg gtgagagcgg gccgtttccc
      721 ttggccacca acgccatgac ggttgccgaa agaacgacca caagggcggc gttggcttcc
      781 tcgctactca acaggttgcc gatcatccct ttctctcctt tctgtagctc tcctatcctg
      841 gcctctagtc tgcggcgtgc gacggatcag gaccacccac agcgcaccca cagtccacct
      901 acattgatac tgtgggcctt tatcgaccgc aatctcggcg cagtatgcgg gcccgatacg
      961 ggggaccggc gcgtgtgatg tcagtcttgg ttaaactgct cgggaaaggc agccgcgaac
     1021 gagaggggag atcttggggc acagtggacg gaccatcgtg gtctcgaaca gggggcccgt
     1081 gacgttctct cgcggcgagt cgggcgagcg tgagcactca aggggagccg gtggtctcgt
     1141 caccgccctc aacgcggtct tgagacgcgc cgagaacgcg gtctggatcg cctcggcaca
     1201 gagcgaggag gacgttgcgg tctcgaagga acccgcgccc tacgaggtcg aagacctgcg
     1261 cgtcaggctc gtcgagcacg atcctgagtc ctacgacctg atgtacaacc acctcgccaa
     1321 cccgctgctc tggttcgtcc atcacggcct ctacaacctg ccttactcgc ccgagctcgg
     1381 ggaggatacc aggcgggcct gggaagaggg ctacgtcccg gtcaaccgca acttcgcgga
     1441 agccgtgatc gaaaccgccg caggcgacga tgcgcccgtc gtcttcctcc acgattacca
     1501 gctgtacatg gcccccctct tcgtcagaga gcgtctcggt gaggacgctc tcctctccct
     1561 gttcgtccac atcccctggc cggagccgga cctctggcgc gtacttccac gctacatcag
     1621 ggagggcgtg ctcgagagcc tcctgtcggc cgacatcgtc gccttccaca cccatcgcta
     1681 cgcccacgcc ttcgccgaga ccgccgccgc cgtactgggc gctgaggttg aggaaggtgt
     1741 gatccactac agaggacgcg aggtgtgggt acgtgcgtac cctatctcca tagacccgga
     1801 ggagttcgag gacctcgcac tcagcgaggc cgtgctcgaa caggagaggt tcgtcaagga
     1861 cctccctgga aagctcctct tgcgcgtgga ccgcacggac ctctcgaaga acgtcgtccg
     1921 cggcttcgtg gcatacggac gcatgctcga gcgccacccc gagatgaagg gcgaggtcac
     1981 tttcctggcc cagctccaac cctcccgcac cgacatcccc gagtacgccg cctacatgga
     2041 ggccgtggga aggaccgccg aggaggtcaa ccagaagcac ggcacgggct cctggaggcc
     2101 catcgagctc tttatggagg atcacttccc ccgctccgtc gccgcctaca agaacttcga
     2161 cgtactgctc gtcaacgccg tccgcgacgg catgaacctc gtcgccaagg agtctgccgt
     2221 tatcaacgag aagaacggtg tactggtgct ctccgagaac gcgggcgccc acgaagagct
     2281 tggggagtac gccctcaccg tcaaccctta cgacatcgac gagcaggccg acgccataca
     2341 cgaggcgctc accatgcccg aggaagaacg cgaacgcagg tctcgcgccc tgcacgacac
     2401 cgtcatgtcc aacaccatag aacactgggt agaggcgcag atgaaggaca tagaggcgta
     2461 tcgggaacgg taaggtacat gcgtatcttc ttgatggccg ccgctgttgt ggtcgggctc
     2521 gcgaacgcga ccctgtatag tttaataggc aacaacacat tcgatgatct cttcgagtgg
     2581 cagaccgacc cgtggtccct ctacgtcatg tacgcgctct gcgccgtctt cgttgggctt
     2641 ttgatctcgg gcggccttct caagtttgcg gagagggatc ttcaagaccg cttcttcgcg
     2701 cgttacgggg tgatggtgct tgcgatctgt atcgggggag cgatgctggc tatcttcctg
     2761 actaccgcca cgttcatgtt cgacgagaat gcatacgtgc cgaggcgtgt ctcagagacc
     2821 ttctacacac tcgccctggt caccatacca ggggcgatgc tgggggcgat agaaggagta
     2881 gtcctggcct tccccctggc ctgggtcctt ggcctgttca ggaagcggag ctagccttcg
     2941 cgcagaagcg cccgcagcac cccggcgacg ccatcgacgc cgtcaacgac gatgtcggcc
     3001 tcggtctcga tctccggcgg gccttcgccg ctcgccaccc cgacgcgaag gatctcccgc
     3061 agagcgcctt cctcgcgcag cttctccagc tcgcggaatg cgtcgaggtc cgtggtgtcg
     3121 tcgccgatga acatggccat gtcgggacgg tattcctcga ccagagtgcg gacggcggtg
     3181 cccttgtcgg ccttgatggg tggacgcgcc tcgacgacgt cgcgtccgac cgtgatcctg
     3241 agccccaggc gctctccttc gcgcttcacg aactcgacgg accgctcgcc gacctcacgc
     3301 ggcgcgtttc ggtagtggac ggaggccgtt atgcccttct cctcgacgag ggcaccctcg
     3361 ggggtgagct cctccctcgc catccgctcc agctcctgga ccctatccag atagggcagg
     3421 gcgtcgggga tcacctcaac ctcgccgtcg cgcaggatct cgaacccgtg gttgccgaag
     3481 tacagcacgt cttcgagccc gatgagctcg taagcgtcct ccgtcttccg tccgctgatg
     3541 ccggccacaa gcaagacctt ctcgctcagc cgtctcagca agtccctgag ctcctcagag
     3601 acctcggaca tgtcaggcgt aggcacgatg ggggcgagcg tcccgtctat gtccgtgagc
     3661 accgccaccg aaccaggctc ctcgccccag gtcctcagac gctcccaggc ttctctacca
     3721 tgactgttca agacataccg tacggcacgc catcccgacg ctgacgtcga tctacatgcc
     3781 gttctcgcgg ccctgggccc gggcgcggat atcctcggtg tcgatctccc cgtcgaggta
     3841 tttgccgatg aagtagccga taatgccgaa gagcacgacg acgagtgtct cctcgaagcc
     3901 gatcaaaaac atcgagaaca ccacgagggc cccgatcacg gcgccccaat gtttgttggt
     3961 ccaggtggcc atctcctacc ttcctttcac taagaagcgg ttcctcttac cgtgacttcg
     4021 acttccttta cgggcacgct ctggtcgtgg agtacgctat ggatgttctc cctggcgcgc
     4081 gtcgcgagct cggagtagtt ctcagacgag gggacctgta tcccggcgaa aacgatgtag
     4141 gagtcgttgt ctgaagcgag agaggccgaa ggggactcgg cccccgcctc cctggctgcg
     4201 ctctcggcga gcgtcttcac ggcgttggca gtgatgacgg tctccttacc gggcgtatcg
     4261 tccatcacgg tgctcctggt gaccctgcgc ccgaaggtga gctccctgag cagcaggaga
     4321 agcgcgatga gagccacgag cgctccgacg acagcgatga tgacccgtgt ggttatcgtg
     4381 agagaggagg ccgagaaccc ttcgagggcc tctagcgcca ccctgtatcc cgtgtactgg
     4441 tttacgacgt cagcagggag gacaccgtag gtaatgagta gcagcacgat ggggaccgca
     4501 agcagcagca gggacagtat cagaaggatg agacggttga aggcgttcac tgcaccctcg
     4561 ttttcgtctg tctcgggtcc gcttcgacga gcttgacgtt gagtttgctc accggcaacc
     4621 ccctctcgga g
//
LOCUS       DATAIR010000298         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_434469, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000298 DATAIR010000000
VERSION     DATAIR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434469"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VHM69_07260"
     CDS             <1..450
                     /locus_tag="VHM69_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740229.1"
                     /translation="HRGPNGLAPDYAATLSELDPLDFARVSASEGEPDLPWMLAAETL
                     SAAVSPARAYHLDHHAYAMIGDPETEVVTLTALVVPRVHRRKGWGNRIMQALYAAFAK
                     QSLAIPATVPEDLAPAFFTRCGWRLQDTSQLEMGLELSQRLEGGTDD"
     gene            443..1678
                     /gene="hutI"
                     /locus_tag="VHM69_07265"
     CDS             443..1678
                     /gene="hutI"
                     /locus_tag="VHM69_07265"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX2740230.1"
                     /translation="MTEVSLLIHDLEAAVTPIGTGPLRRDEFGAMRVHSPASIAIAGD
                     RILAVGPPEEVLRQIPSNSHYESLDGRGKVALPGLVDCHTHAAFLGDRAEEFELRSSG
                     ATYEEIHASGGGIRSTVAATRAGSEEDLTSAVERHLGWMLDHGTMTAETKSGYGLDRE
                     TELKSLRAIHAAGQSSPVDVYPTFLGAHTVPPEFDGAAGYVEFVIRDVLPEAAPLTRA
                     ADVFLERGSFEAPEARRYLEACREHGLALRLHADQFSERGALPLAIEMGARSVDHLEA
                     TGDEGARRLARSDVAAVILPACALFLDLPLPPARLLADEGGIVALATDFNPGSSFCES
                     LPLVMNLACTRLGLSPAEALCACTANAAHVLGLEDVGRLAPGYMADVLLLDAPDWRHV
                     AYHLGGDRFAAKIKSGRVL"
     gene            1713..2069
                     /locus_tag="VHM69_07270"
     CDS             1713..2069
                     /locus_tag="VHM69_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740231.1"
                     /translation="MPTTVRIRPEVITAHRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     RGWVYAGEPDFIYRQIREVVIALPDITFEPNAIEESVKTVLEKARTEEEREEGRLLLH
                     QAFEKTGQLAEAEEFL"
BASE COUNT          385 a          705 c          646 g          339 t
ORIGIN      
        1 catcgcgggc ccaacggatt ggcccccgac tatgcggcca cgctctccga actcgacccc
       61 ctggacttcg cacgcgtctc ggccagtgag ggcgaaccag acctgccgtg gatgctcgcc
      121 gcggagacgc tctcagctgc ggtctctcca gcgcgggcct atcacctcga ccatcacgct
      181 tacgcaatga tcggagaccc ggagacggag gtcgtaaccc tgaccgcgct cgtcgtaccc
      241 cgtgtccatc gtcgcaaggg ctggggcaac cgcatcatgc aagccctgta tgcggcgttt
      301 gcaaaacagt cgttggcgat ccctgccacc gtccccgaag atcttgcccc agcgttcttc
      361 acgcggtgcg gctggaggct tcaggacacg agccagttgg agatgggcct ggaactttcg
      421 caaagactag aaggcggcac ggatgactga ggtctcgctc ctgatccacg acctcgaggc
      481 cgccgtcacg ccgattggga ccggaccgct tcggagagat gagttcggag ccatgcgagt
      541 ccactccccc gcttcgatag ccatcgccgg ggaccgcatc ctcgcggtcg gcccgccgga
      601 ggaagtcctg cggcagattc catcaaactc ccattacgaa agtctagatg gacgcgggaa
      661 ggttgccctg cccggtctcg tcgattgcca cacgcatgcg gccttcctgg gagacagggc
      721 ggaggagttc gagttgcgct cctcaggagc tacctacgag gagatccacg cttccggcgg
      781 cggcatccgc tcgacggtgg ctgcgacgcg ggccggtagc gaggaagacc tgacatcagc
      841 cgtcgagcgt cacctcggct ggatgctcga ccacggaacg atgaccgcgg agacgaaatc
      901 aggctacggc ctcgaccgcg agacggagct gaagtccctg cgtgcgatcc acgccgcggg
      961 acagagtagt cccgtggacg tatatccgac cttcctcggg gcgcacacgg tcccgcccga
     1021 gttcgacggc gcagcagggt acgtggagtt cgtgatccgg gatgtgctgc ctgaagccgc
     1081 acccctcacc cgcgcagccg acgtcttcct ggagcggggc tctttcgagg caccagaggc
     1141 gagacgctac ctcgaagcgt gcagggagca cggactcgcg ctccggttgc acgccgacca
     1201 gttctccgaa cgaggggcgc ttccactcgc aatcgagatg ggcgcgcgca gcgtggacca
     1261 cctcgaagcc acgggggatg aaggagcccg ccgtcttgcc aggagcgacg tcgcggctgt
     1321 gatcctcccc gcgtgcgcgc tcttcctcga cctacctctc ccccccgcgc gcctcctcgc
     1381 cgacgagggt gggatcgtcg cgctcgccac ggacttcaac cccggtagct ccttctgcga
     1441 gtcgctgcca ctcgtgatga acctggcgtg cacccgcctc gggctctccc cggcagaagc
     1501 cctctgcgcc tgtacggcga acgccgcgca cgtcctcggc ctcgaagacg tcggcaggct
     1561 cgcaccgggc tacatggccg atgtgctcct cctcgacgcc ccagactggc gtcacgtcgc
     1621 ctaccacctc ggcggggaca ggttcgcggc gaagataaag tcagggcgag tcctctaaca
     1681 gccagccgac tcgtcgctgc taaactgtcc acatgccaac gacggtacgc atcaggcctg
     1741 aggtcataac cgcccacagg ctcaggatcg agatgttcgg cctcgaagac gaggacatag
     1801 agaacaccat ccgcatgaag ggatgggcct gggtcctcgc ccgccgcggg tgggtatacg
     1861 cgggtgaacc cgattttatc taccgtcaga tcagggaagt cgtcatcgcc ctgccggata
     1921 tcacattcga acccaacgct atcgaggaat ccgtaaagac cgtgctggag aaggcccgta
     1981 cagaggagga acgcgaagag ggacgcctgc tcttgcacca ggccttcgag aagacaggcc
     2041 agctcgccga ggctgaagaa ttcctgtagc cgcct
//
LOCUS       DATAIR010000299         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_54831, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000299 DATAIR010000000
VERSION     DATAIR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54831"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VHM69_07275"
     CDS             <1..448
                     /locus_tag="VHM69_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740232.1"
                     /translation="QISGRGGDDVLLGRRGSDAMDGGTGRDAVLGGNEFGPLSGDKTL
                     DGGKDGDFVGGAYGSDSLAGGGGKDYLTLGPIDDFAQDVASGGGGNDAIFAKNIHADK
                     DIVDCGPGFDRALVDSKDITSRCEREYTKTSRFFRAISPNYFAPLP"
     gene            complement(557..868)
                     /locus_tag="VHM69_07280"
     CDS             complement(557..868)
                     /locus_tag="VHM69_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740233.1"
                     /translation="MPELNCTVRRVGDYESRTEEYEVAFAVGESTPPRGLRIKYQAAR
                     AYEINAALDAAVEVARAAISDADPNLKGPTHEAISKFSWRVIQSSKYEEGGARSSSLE
                     F"
     gene            954..2921
                     /locus_tag="VHM69_07285"
     CDS             954..2921
                     /locus_tag="VHM69_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2740234.1"
                     /translation="MNNFFETSKKTSKGRPLGRFVRGLRALFNLALILGVAAVIGASC
                     LYLFVIKEYETRLDRTYPELAENSYIYDVEGNKVGEIPVIESRETVGFEGLGEHLPEA
                     VIAVEDRRFYDHFGVDFEGLARAAWTDLRSMEVQEGGSTITEQLMKNLYVPEDERFVT
                     SFRRRFVQSALAFAYERKHTKNEVITAYLNTVYFGGGAYGAEAAAQHYFGKSADELSL
                     SEAATLAGFLHAPSTYSTWEGDVLVDSARARRDRVLRLMQEQGIISSSARDEAEAAPM
                     EFAPDPPPEDSAYGPFFDKVRREVEERLGPRALALGGLRIHTTMDPALQHAAVETSED
                     VLFAPDDPSAAVVTVEPQSGAIRALAGQEGDFNLALDARRQPGSSFKPVVLAAALRED
                     ISPESIYVSHALAFDFADEHYVINNYDFVERGEITVSKAMAESDNTVFVQLAADVGLD
                     NVVRTAGDLGITTQVEPYPSTAIGGLGTGVSPLDMASAYSTFAGGGIHREPYSIENID
                     RNSYGESETVYDHRIGGRRVLTGNEAAVANQVLRGVVEEGTATMFHDLDEEIGLPSAG
                     KTGTTDNFADAWYVGYTPRLCTSVWVGYPDGRRSLVGVHGLAEPNGETLPMDIWAAYM
                     ARATEGDLSLDFPEADTSDLNILYGGYSDGF"
     gene            3096..3542
                     /locus_tag="VHM69_07290"
     CDS             3096..3542
                     /locus_tag="VHM69_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740235.1"
                     /translation="MDLQRLYAGSFIDWEAVAASWNKRTVPSRLLLFAARRYLEEKTR
                     VPDPERVELLAPARLPDEIKRAFASPPDTPASSRWGEFVDAAVAAELEMVSYGERPPL
                     LHELRLGLEQAASEAGEGTDAGRWFLARRDALPGTDLPEDPGYLPI"
     gene            3741..5186
                     /locus_tag="VHM69_07295"
     CDS             3741..5186
                     /locus_tag="VHM69_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX2740236.1"
                     /translation="MESIPNASYSLTLRVDFPHEAGALGKILTVIGEADGLVGAVDIV
                     RMRSDRTIRDITVNARDSEHGQQIVQVVENLPQVSVVNVSDRTFLMHLGGKIEVRSKL
                     QIRTRDDLSMVYTPGVARVCRAIANDPERAFNLTIKRNTVAVVSDGTAVLGLGDIGPQ
                     AAMPVMEGKAALFKQFAGVDAFPICLDTKDADEIVETVKHLAPAFGGINLEDISAPRC
                     FEIEERLKKELDIPVFHDDQHGTAVVVLAALINSLKIVGKKVEDLRIVVNGVGASGVA
                     CAKIMMAAGAKNIVGCDSKGIVHEGREDLNESKRWFAGNTNPEGRTGDLSEAVTGADL
                     FLGLSVPDVLTLDHVESMNPDPIIFAMANPDPEIRPEVAIGKARIIATGRSDYPNQIN
                     NVLCFPGIFRGALDVRAREIDEPMKLAAAEAIAGVIPEEDLSEDYIVPSVFDERVAPA
                     VAEAVAETAKETGMARRIQERAETGLTATSF"
     gene            5236..5511
                     /locus_tag="VHM69_07300"
     CDS             5236..5511
                     /locus_tag="VHM69_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX2740237.1"
                     /translation="MRKLRLIFVCATPGEGRCGAKGGGELLAAFKDEVERLGIPPSSV
                     LRNACTRRHEEGPVVFVYPDDVWYTRVRPEEVSEIVERHLAPLRVEG"
     gene            complement(5583..>5862)
                     /locus_tag="VHM69_07305"
     CDS             complement(5583..>5862)
                     /locus_tag="VHM69_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740238.1"
                     /translation="SRRGRPPFRFFDRGYVVSLGPDHAVADAVGLRLRGAPAQALYRS
                     VLLYYMKSRRDRILTGADWAMERTLGRVGFGTRQPSSDGGEEDLEGEG"
BASE COUNT         1108 a         1870 c         1873 g         1011 t
ORIGIN      
        1 ccagatctcc ggtcggggcg gtgacgacgt gttgctcggg cggaggggaa gcgacgccat
       61 ggacggaggg accggcaggg acgccgttct cggcggcaac gagttcggtc cgctaagcgg
      121 cgacaagacc ctcgacggcg gcaaagatgg cgacttcgta ggcggcgctt acggctccga
      181 ctccctggca ggcggaggcg gaaaagacta cctgaccctc ggtccgatcg acgacttcgc
      241 acaggacgtt gcctctggtg gcggtggaaa cgatgccatc ttcgccaaga acattcatgc
      301 cgacaaggac atcgtagatt gcggtcccgg cttcgacagg gcgctcgtcg acagcaagga
      361 catcaccagt cgctgcgaga gggagtacac caagacatcc aggttcttcc gcgccatctc
      421 gccgaactac ttcgccccgc tcccataacc tggtagggtg ctcttcagcc tgaaactacc
      481 cgacaggggg ctctaaaaag ggccccctgt ccctttcaag aagtagtcgg gctggcagtg
      541 tacgcaggcg tcttcatcag aattccaggc tggaagacct ggccccgccc tcttcgtact
      601 tggaggattg gatcacacgc caggagaact tggagatggc ttcgtgggtg gggcccttca
      661 agttcgggtc ggcgtcggag atggccgctc tcgccacttc gaccgcggcg tcgagggcgg
      721 cgttgatctc gtaggcccgc gctgcctggt atttgatgcg cagcccgcgc ggcggcgtcg
      781 attctcccac agcgaaggcg acctcgtact cctcggtacg gctctcatag tctccgacgc
      841 gcctgaccgt gcagttcaac tccggcatga ggtcatccta ccatttgcgt gggagagcag
      901 gcgcgccccg caccggcccg gacccgattg gggtagacta cggccggagg gccgtgaaca
      961 acttcttcga aaccagcaag aagacgtcca agggacgtcc tctcggacgc ttcgtacgag
     1021 gactacgtgc cctgttcaac ctggctctca tcctcggcgt agccgccgtg ataggcgctt
     1081 cctgtctcta cctgttcgtc atcaaggagt atgagaccag gctcgacaga acctaccccg
     1141 agctcgccga gaactcctac atctacgacg tggaggggaa caaggtaggg gagataccgg
     1201 tcatcgagag ccgcgagacc gtcggtttcg aagggctcgg ggagcatctc cccgaggcgg
     1261 tcatcgccgt cgaggacagg cgcttctacg accatttcgg ggtggacttc gagggactgg
     1321 cccgcgcggc atggacggac ctgaggtcca tggaggtgca ggagggcggg tccaccatca
     1381 cggagcaact catgaagaac ctctacgttc ccgaggacga gcgtttcgtg acctcctttc
     1441 gcaggcgctt cgtacagtcc gcgctcgcat tcgcctacga gcgcaagcac acgaagaacg
     1501 aagtcatcac cgcctacctc aacaccgtgt acttcggtgg cggcgcttac ggggccgagg
     1561 cggcggcgca gcactacttc ggcaagagcg ccgacgagct cagtctttcg gaggcggcca
     1621 cgctcgccgg tttcctccac gccccctcga cctattcgac ctgggagggc gacgtcctcg
     1681 tggatagcgc cagggcgcga cgcgatcgcg tactcaggtt gatgcaggag cagggcatca
     1741 tctcttcaag cgctcgcgac gaggccgagg cggcacccat ggagttcgcc cccgacccgc
     1801 cgcccgagga ctctgcctac gggcctttct tcgataaggt caggcgcgag gtggaggaga
     1861 gactgggacc acgggccctt gcgctcgggg gcttgaggat ccacacgacc atggatcccg
     1921 ccctgcagca cgcggccgtc gagacctcgg aggatgtact cttcgcaccg gatgaccctt
     1981 ctgccgccgt cgttaccgtc gagccccaga gcggcgcgat acgagcactc gcggggcaag
     2041 agggggattt caacctcgcg ctcgacgcca ggcgccaacc tggcagctct ttcaagcccg
     2101 tcgtgctcgc cgccgccctc agggaagata tctcccccga gagcatctac gtctcccacg
     2161 cgctggcttt cgacttcgcg gacgagcact acgtcatcaa caactacgac ttcgtcgagc
     2221 gcggggagat cacggtctcc aaagcgatgg cggagtcgga caacacggtc ttcgtgcaac
     2281 tcgccgccga cgtcggcctc gacaacgtcg ttcggaccgc gggagacctc ggcatcacca
     2341 cgcaggtcga gccgtaccca tctacggcga taggaggcct gggtaccggc gtcagcccgc
     2401 tcgacatggc ctcggcctac tcgactttcg ccggcggcgg tatccatcgg gagccttatt
     2461 ccatcgagaa catcgacagg aacagctacg gcgagagcga gacggtatac gaccacagga
     2521 tcggcggaag gcgcgtgctc acgggcaatg aggccgctgt cgcgaaccag gtcctcaggg
     2581 gcgtggtcga agaggggacg gctaccatgt tccacgacct tgacgaggag ataggactgc
     2641 cctccgcggg caagactggc acaacggaca acttcgccga cgcatggtac gtgggttata
     2701 cgcctcgcct ctgcacctcc gtgtgggtcg gctatccgga cggcagacgc tccctcgttg
     2761 gagtccacgg gctcgcggag ccgaacgggg agaccctgcc gatggatatc tgggcggctt
     2821 acatggcaag ggcaacggag ggggatctgt cgctcgattt ccccgaggcc gacacgagcg
     2881 acttgaatat cctctacggc ggctactcgg acggcttctg agcgccccaa caggacacga
     2941 agcatcgctt atggtggttc tcgaaaagga tgagctacgc cttcggagga ggcatcaggc
     3001 tacaggtttc aggcttcagg tctttttgct tatgcctgat gcctttactc acctcacgtt
     3061 cactctttca gcaaaccgct ctagaatgag cggctatgga cttgcagagg ctctatgctg
     3121 gatcctttat agactgggag gcggtcgccg catcctggaa caaacgcacc gtcccctcgc
     3181 ggttgttgct cttcgccgca cgccgctacc tggaggagaa gactcgtgtc ccggacccgg
     3241 agcgcgtcga gttgctcgcg ccagcccggc tccctgatga gataaaacgc gccttcgcct
     3301 caccccccga cacgccggcc tcttcccgat ggggcgagtt cgtggacgcc gccgtcgccg
     3361 ccgagctcga gatggtttcg tacggcgaga gaccgccgct attgcacgag ctgaggttgg
     3421 gtctcgaaca ggccgcctcg gaggcgggag aaggcaccga tgcagggaga tggttcctcg
     3481 cccgccgcga cgcacttcca ggcacggatc tccccgaaga tccagggtat ctgccgattt
     3541 gaggcttcag gtttcaggca aagggttgtt ggcggggcgg tccactgtgg ccatcgaggg
     3601 acggcgacaa tgattttgct gaaacctgac tgctgatgcc tgaagtcttc atagccattt
     3661 accgtggttc gcgactgcaa tacaatcacg ctcattgaga cccgcgtggg gcgggttcac
     3721 gagggagaga ggtaacagaa gtggagtcga tacccaatgc aagctacagc ctgaccttgc
     3781 gggtagattt cccgcacgag gcgggagccc tcggaaagat tctcacggtc ataggggagg
     3841 cggacggcct ggttggcgcg gtcgacatcg tgcggatgcg cagcgacagg accatccgcg
     3901 acatcaccgt caacgcccgc gactccgagc acggccagca gatcgtccag gtcgtcgaga
     3961 acctgcccca ggtcagtgtc gtcaacgtct cggacaggac gttcctcatg cacctcgggg
     4021 gcaagatcga ggtccgctcc aagctccaga tccgcacccg cgacgacctc tcgatggtct
     4081 acacgccggg cgtcgcccgc gtctgccgcg cgatagccaa cgatccagag cgggccttca
     4141 acctgacgat caagcgcaac accgtcgccg tcgtctcgga cgggacggcg gtgttgggcc
     4201 tgggcgacat cgggccgcag gcagcgatgc cggtcatgga gggcaaggcc gcgcttttca
     4261 agcagttcgc cggcgtcgac gctttcccga tctgcctcga caccaaggac gcggacgaga
     4321 tcgtcgagac cgtgaagcac cttgccccgg cgttcggcgg tattaatctg gaggacatct
     4381 cggccccacg gtgcttcgag atcgaagagc gcctcaagaa ggagctcgac atccctgtat
     4441 tccacgacga ccagcacggc acggcggtcg tcgtgcttgc cgccctgatc aactccctca
     4501 agatagtcgg caagaaggtc gaggacctcc gcatagtggt caacggcgtc ggcgcttccg
     4561 gtgtggcgtg cgccaagatc atgatggcag cgggcgcgaa gaacatcgtc ggctgcgact
     4621 cgaagggtat cgtccacgag gggcgcgagg acctcaacga gtcaaagcgg tggttcgcgg
     4681 ggaacaccaa cccggagggc aggaccggcg acctctccga ggccgttaca ggggccgacc
     4741 tcttcctcgg gctctccgtg cccgacgtcc tcaccctgga tcacgtcgag agcatgaacc
     4801 ccgacccgat catattcgcc atggccaatc ccgaccccga gataaggcct gaggtagcga
     4861 tagggaaggc caggatcatc gccacgggac gcagcgacta cccgaaccag atcaacaacg
     4921 tcctctgttt ccccggcatc ttcaggggcg ccctcgacgt aagggcccgc gagattgacg
     4981 agccgatgaa gctcgccgcg gccgaggcga tagcgggcgt tatccctgaa gaagacctct
     5041 ccgaggacta catcgtgccc tccgtcttcg acgagcgcgt cgcacccgcc gtcgccgaag
     5101 cggtggcgga gaccgcgaag gagacgggga tggcgaggcg catccaggag cgtgcggaga
     5161 cgggtcttac ggcgaccagt ttctaggggc agccgcgtct ctggacttct cagggacgcg
     5221 gcatagaatc tgggtatgag gaagctgcgc ctgatcttcg tgtgcgccac gcccggcgag
     5281 ggacgctgcg gggcgaaggg cggcggcgaa ttgctggcgg cgttcaagga cgaggtggag
     5341 cgactcggca tcccgccttc ctccgtcctc cgcaacgcat gcacccgccg ccacgaggag
     5401 ggtccagtcg tcttcgtcta tcctgatgac gtctggtaca cccgcgtacg tccagaagag
     5461 gtgtcggaga tagtcgagcg gcaccttgcc ccgttgcggg tcgagggcta gcctgaggct
     5521 ctccccacga acagtgggtt gaataacgca gccgtcacgt tgccgatcca caggaacccc
     5581 ggtcagccct cgccttcgag gtcttcttca ccgccgtcgg atgatggctg gcgggtaccg
     5641 aaacccacgc ggcccagggt ccgctccatt gcccagtcgg cgcctgtgag tattctgtcg
     5701 cgcctgctct tcatgtagta gaggagcacg ctgcgataaa gggcctgcgc cggggcgcca
     5761 cgcagtctca ggccaacggc gtcggccacg gcgtgatccg gacccaggct cacgacgtag
     5821 ccccggtcga agaagcggaa gggcggcctg ccgcgccggc tc
//
LOCUS       DATAIR010000300         5306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_296576, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000300 DATAIR010000000
VERSION     DATAIR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296576"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VHM69_07310"
     CDS             complement(<1..736)
                     /locus_tag="VHM69_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX2740239.1"
                     /translation="MAENLVGKILKDHMVEGSLDPDDEVGLRIDQTLLQDATGTMACL
                     QFERMGVDRAQVDFAVQYVDHNVIQLDFKNPDDHRFLQAFAARYGIHYSRPGNGICHY
                     LHVERFAKPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQTPAPKVVGVE
                     LTGALPQWVTPKDVILELLRRRGVRGGLGRIFEFYGEGVANIDVTGRTTICNMIAELG
                     ATTGVFPSDERTKEWLRMQQRLDDFVE"
     gene            complement(1087..1473)
                     /locus_tag="VHM69_07315"
     CDS             complement(1087..1473)
                     /locus_tag="VHM69_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740240.1"
                     /translation="MGVASASPDDRHGTVTPLYRAPAKGLDGIQRPRCEFVDIFSGRG
                     CGDPGEERHQGRLLCPTHAKILALEHRMGILLRSLAVLDDWLDDNAGKVTDEVRVRRM
                     KRWQLEVLEQLDSTGLQLEAVRQEVE"
     gene            complement(1476..1682)
                     /locus_tag="VHM69_07320"
     CDS             complement(1476..1682)
                     /locus_tag="VHM69_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740241.1"
                     /translation="MATLVCTCPHGHRLWAEFWWVGREHRWVFFDDDETSETYTEQVT
                     HCPGCGRLLERKNLKGSSPFVRQG"
     gene            complement(1810..2541)
                     /locus_tag="VHM69_07325"
     CDS             complement(1810..2541)
                     /locus_tag="VHM69_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEX2740242.1"
                     /translation="MGALAAGFWGFVGGASLLIGALVGIYANASRRTIATIMALGAGV
                     LISSVAFELMDEAYRLGGFDASAFGLLLGALAFFFADRQVNRRGGTRRKNSADKQGDS
                     ATAIAIGALLDGIPESAAIGISLLGGGEISAALVAAVFLSNVPEGLSSASGMKQAGRS
                     IPHIIGLWGGVTMVSAVAALLGYFFLAGAPANVVASIQSFAAGAILTMLASTMMPEAF
                     EEGGEVVGLVTAVGFLLSFVLSHLE"
     gene            complement(2563..3153)
                     /locus_tag="VHM69_07330"
     CDS             complement(2563..3153)
                     /locus_tag="VHM69_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740243.1"
                     /translation="MSNVALIVIIVVVLIVILLLALYFASRRRAAQRSEEQRERTREE
                     FGPEYERTVRERDSEEEAERELRERRGSVEREVEPLSDESRARYEEQWGEVERVFVDN
                     PERSIEMADRTVSDIMNERNFVSHAGQSDEETERRLGAMHPDVADDYREARRIRAVVV
                     ARSAGHADESSEEESTEDLRQAIRKYRAVYQRLVRE"
     gene            3339..3515
                     /locus_tag="VHM69_07335"
     CDS             3339..3515
                     /locus_tag="VHM69_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740244.1"
                     /translation="MTESRPGGTEDNVRDELDEAAERGKSASKEAVEKEEETGGTGGV
                     KDNVHDEWDESQKH"
     gene            complement(3689..4120)
                     /locus_tag="VHM69_07340"
     CDS             complement(3689..4120)
                     /locus_tag="VHM69_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740245.1"
                     /translation="MPRTYIIGLILFCIALNAAGFLWKLFEPIPLYDEIAHFVTPFTL
                     VMILAEVIYRGGGDDEFFNTPVRAIITGAVIGLLGAAGWEGVEVVMSMMGLSIANALP
                     DTIFDIVLGVLGGAAGAWAADRYLDRIFRRSRTNPHQPRVR"
     gene            4599..>5306
                     /locus_tag="VHM69_07345"
     CDS             4599..>5306
                     /locus_tag="VHM69_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740246.1"
                     /translation="MGKRVIVLFASMALALALSSGAAFAHPLMFESDLTENGIVNFCN
                     PEQIKPVLFEDAISDWNSYASDNSFPRVVDVTGNQGAFCELRADAQGGDSAGYFARVV
                     FNAHPDNIDVSTRINDLSVTLRGAVLRHELGHAVVGLDHNQLCSSSIMPTLRFCNENE
                     TPRRTTVGPHDADDRSEYWSSGDPPLYPIHDKCWTNEDADGDGVCDRHGPPTAFNRSA
                     TLYSVSSVSDQPMWAPPA"
BASE COUNT         1063 a         1688 c         1533 g         1022 t
ORIGIN      
        1 gttccacgaa gtcgtcaagg cgctgctgca tccgcagcca ctccttcgtc ctctcgtccg
       61 aggggaagac gccggtggtg gctccgagct cggcgatcat gttgcatatc gtggtgcgtc
      121 cggttacgtc tatgttcgcg acgccttctc cgtagaactc gaagatgcgc cccaggccgc
      181 ccctcacgcc gcgccgcctc aagagctcca ggatcacatc cttcggcgtc acccactgag
      241 ggagagcccc ggtaagctct acgccgacaa ccttgggagc cggagtctgg aagggctggc
      301 cggccatcac gagggccacg tcgaggccgc cggcccctat ggcaatggag ccgagagcgc
      361 cggaggaagt cgtatgagaa tccgccccga cgagcgtggc gccaggtttt gcgaaccgct
      421 cgacgtgcag gtagtggcag atgccgttac cagggcggga gtagtggatg ccgtatctgg
      481 cggcgaaggc ctgcaggaaa cggtggtcgt ccgggttctt gaagtcgagt tggatgacgt
      541 tgtggtcgac gtactgcacg gcgaagtcca cctgagcgcg gtcgacgccc atccgctcga
      601 actgcaagca ggccatcgtg ccagtcgcat cctgaaggag tgtctgatct atcctcagtc
      661 caacctcgtc gtcgggatcc agcgacccct cgaccatgtg gtctttgagg attttcccga
      721 caaggttctc cgccatggca aacccccgac atactttccc tgacctgccc acttattcta
      781 cacacctgcg cttccgagcc agcccgacac cttcggttcc gctggatcgg atgtaccggg
      841 aatggtccgt tcgagtgatg tgccctcccc taatggcatg ctgatctaca ccagatatgg
      901 accccgggga actcgggcag ccggcaaaaa taccggcccc ctgcttgcac ggaagacccg
      961 gcttttgatc ctcatgctgc gccgccatgg agacgcgaaa gctcgggggc gaaggtggaa
     1021 cgcaagatgc atgagattca tggcgatctg gtcaccgtct atgcctgacc ggcactctac
     1081 tatccctcac tccacttcct ggcggacggc ttcgagttgc agtccggtag aatcgagttg
     1141 ctcgagcacc tccaattgcc agcgcttcat acgccgaacg cgcacctcgt ccgtcacctt
     1201 ccctgcgtta tcgtccagcc agtcatccaa cacggcaaga gacctcagca ggatccccat
     1261 tcgatgctcc aacgcgagaa tctttgcgtg ggtcgggcac agcagcctgc cctggtggcg
     1321 ctcctcaccc gggtcgccgc acccccgccc cgagaagata tccacgaatt cacagcgcgg
     1381 ccgctggata ccgtccaacc ccttcgccgg tgcccggtac aggggcgtca ccgtaccgtg
     1441 cctgtcgtcc ggtgaggcgc ttgcgacgcc caccactacc cctgacgcac gaatggggat
     1501 gaccccttga ggttcttgcg ctcgaggagc ctgccgcacc ccggacagtg cgtgacctgc
     1561 tcggtgtagg tctcgctcgt ctcgtcgtcg tcgaagaaca cccatctgtg ctcccgacca
     1621 acccaccaga attcagccca gagccggtgc ccatgcgggc acgtgcacac caacgtagcc
     1681 atcgaccctc ctccttatcg ggtacttgcg tcgatagtaa cacacatgac aatacaaatg
     1741 gcggcgtatg gttcggctgg ccaagaatgt cccaaagtat gtagtgctct gcgcgtttac
     1801 ggtcgtcccc tactccagat ggctcaggac gaaggagagg aggaagccaa cggctgtgac
     1861 gagcccgacg acctcgccgc cctcctcgaa ggcttctggc atcattgtag aagcgagcat
     1921 cgtcaggatg gcccctgccg cgaaagattg gattgaggcg acgacgttcg ctggtgcgcc
     1981 tgcgagaaag aagtagccga gcagcgcggc cacggccgag accatagtaa ccccgcccca
     2041 gagcccgatg atgtgaggaa tcgagcgtcc ggcctgtttc attcccgagg ccgaggacag
     2101 cccttcaggg acgtttgaga ggaagactgc ggcgacgagg gcggcgctga tctcacctcc
     2161 cccgagaagg ctgatcccga tcgccgccga ctcggggatg ccgtccagga gcgcgcctat
     2221 ggcgatggcc gtcgccgagt caccctgttt atctgccgag ttcttgcgtc gtgtccctcc
     2281 cctcctgttc acctgcctgt cggcgaagaa gaatgcaagg gcaccgagca acagcccgaa
     2341 agccgacgcg tcgaagcctc caagccggta ggcttcgtcc atgagctcga aggcgaccga
     2401 ggagatcaag acgcccgccc caagcgccat gatggtcgcg atggtacgcc tggaggcatt
     2461 ggcgtagata cccacgagcg cccctatgag gagcgacgcc cccccgacga atccccagaa
     2521 tccggcagct agagcgccca tgccgccact tcaggaacct ctctactccc gcacgagccg
     2581 ctggtagacc gcgcgatact tgcggatggc ctgccgcagg tcttctgtgg actcctcttc
     2641 cgagctttcg tccgcgtggc cggccgaacg cgccacgact acggcacgga tgcgcctcgc
     2701 ctccctgtag tcgtcggcga cgtcggggtg catggcgccc aggcgccgct cggtctcctc
     2761 gtctgactgg cctgcgtggg agacgaagtt gcgctcgttc atgatgtccg agaccgtcct
     2821 gtcggccatc tctatggacc tctccgggtt gtccacgaag acccgctcga cctctcccca
     2881 ctgctcctcg tagcgtgcgc ggctctcatc agagagcggc tcgacctccc gctccacgct
     2941 cccgcgacgc tccctcagct cccgctcggc ctcctcctca gagtccctct cccggaccgt
     3001 ccgctcgtat tccgggccga actcctccct ggtccgctcc ctctgctcct cgctcctctg
     3061 cgcggcccta cggcgcgaag cgaagtacag tgccaacagc agtatcacta tcaagacgac
     3121 cacaatgatc acgatcaggg ccacgttact catgttttct cctcccatta agttggtctg
     3181 ccctgcattg ttccccgaaa agtaatttgc gtaacgtctt cgcacaggtc tatccggcaa
     3241 aataccggca ccggctcgtg tgccaggctg cggagatccc ctcggggatc gtggtagcat
     3301 gaatcaggca tagccgaagc gaccgagaag gaggacgcat gaccgagagt cgtccgggag
     3361 gaaccgagga caacgtacgc gacgagctgg acgaggctgc tgagcggggc aagagcgcct
     3421 cgaaggaggc cgtcgagaaa gaggaggaaa ccggcggcac aggaggcgtg aaggacaacg
     3481 tccatgacga gtgggatgag tcgcagaagc actagagcga ttttcagatg tacttctgca
     3541 aatcgcggca tcaggaatcg aacttttgat tattgctgaa gagttgagcg cgttgcaaag
     3601 aggggttcaa cctctctttg caaagtgctt ttgacatcgt agccgcgtgg gagaggcgcc
     3661 ggagcgtaag ggctcaggtc tattccttct aacggacgcg gggttggtgg gggttcgtac
     3721 gcgagcgcct gaagatacgg tcgaggtagc ggtctgcggc ccacgcaccg gccgcgccgc
     3781 cgaggacgcc gagaacgatg tcgaagatcg tgtcaggcag ggcgttggct atgctgaggc
     3841 ccatcatgga catcacgacc tcgacgccct cccaaccggc ggcgccgagg agcccgatca
     3901 ccgcccccgt gatgatggcc cgcaccggcg tgttgaagaa ctcgtcatct cccccgccgc
     3961 ggtaaatgac ctcagccagt atcatcacga gcgtgaacgg cgtgacgaaa tgggcgatct
     4021 catcgtatag cggaatcggc tcgaagagct tccacaagaa cccggccgcg ttcagagcga
     4081 tgcagaacaa gatcagacct ataatgtacg ttcgcggcat tatccctgcg cctccgtgta
     4141 gatctcagtc cccaataccc cggggactct gtatttatac gctttcccag ccacaaacac
     4201 actggccctg cgtttcagcg cccggcctgt agagaggact cagcaattct tctatcgata
     4261 gtctagctga ggtcgttggg cacaacctcg tcatgtgatg cgtaaatgca ccgcatattc
     4321 gaacttcgcc actctgccgc gctgagaatt gagccggaga tcgagctacg gttacacgag
     4381 ctcttgcgcc tgcataaccg cctcacgtga gcgtgggcaa attaccagtg agtgatgtcc
     4441 gatattggtc ccaggaacgt attccatcca atactgaaaa atagctcatt tggggtatgt
     4501 gtcgttgcac cagggatgct acgctcaagg gcaagagtca caatattcta tcgttgcgcg
     4561 atcaccattg ttgcgcaatg ggagaaaggt acgagacgat gggaaagcgg gtcattgtct
     4621 tattcgcgtc gatggctttg gcactggcgc tatctagcgg tgccgcgttt gcccatcccc
     4681 tgatgttcga gagcgacctt actgagaacg gcatagtcaa tttttgcaat cctgaacaga
     4741 tcaagccagt ccttttcgaa gatgctatct cagactggaa ttcctacgct tccgacaatt
     4801 cctttcccag ggttgtcgac gtcaccggta atcagggtgc tttctgtgag ctgagggcgg
     4861 acgctcaggg tggagactca gcaggatact tcgcccgtgt cgtcttcaac gcccaccctg
     4921 acaatataga cgtttccacc cgcatcaatg acctctcagt gactctgagg ggcgccgttc
     4981 tgcgccacga actcgggcac gcagttgtgg gcttagatca caaccaattg tgctcatcgt
     5041 cgatcatgcc gacattgcga ttctgcaatg aaaacgagac gccgcggcgc acgacggtcg
     5101 gaccccacga cgcagacgac cgcagcgagt actggagctc tggagatcca cctctctacc
     5161 caatacacga caagtgctgg accaacgagg atgcggacgg cgacggcgtg tgcgaccgtc
     5221 atggcccgcc gacagcgttc aacagatcgg caaccctgta tagcgtcagc tcggtcagcg
     5281 atcagcccat gtgggcgcca ccggcc
//
LOCUS       DATAIR010000301         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_19352, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000301 DATAIR010000000
VERSION     DATAIR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19352"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHM69_07350"
     CDS             <1..485
                     /locus_tag="VHM69_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX2740247.1"
                     /translation="LRDLKAKGELPRDLVLKVSVQLAAANAAAVKAMEDLGANTYNVP
                     TDLSLAQLAAIRAAAELPLDVYVEVPDDFGGFVRHYEIPDLVRVASPVFVKFGLRNAP
                     NIYPSGTHLEATAIALGRERVRRARIGQEMLERYYPEAKTTEKGATFPGIPVETKERA
                     "
     gene            482..1645
                     /locus_tag="VHM69_07355"
     CDS             482..1645
                     /locus_tag="VHM69_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX2740248.1"
                     /translation="MKITHVDTFVVGTEWRNLTIIRAHTDEGLTGLGEARMVNHTDAL
                     VGYIAEAAPRHLVGHDPFRIEDLVKKMGRDDFSRAGEVMSSGIAMFETACWDIVGKAL
                     GQPVYNLLGGPVRDRVKAYANGWYRVERTPEEFHAAAKKVVERGYRALKLDPFGPGHY
                     ELEYEEKIKAVSLVEAVRDAIGPEVEILLEMHGRFSPATAIEMARMLHPFGLGWVEEP
                     VPPVNLKALKKASDGIEATVATGERIHQRLEYREVFELQAADVIQPDISHIGGLLETK
                     KLASWADAYYVTIAPHNVGGQINTAAALHLAACTTNFRIQEYFNDFADPWVRETAPGL
                     PEVVDGYFELPKGPGLGVELDEEVIEAHPKQNVHFNLFAEGWERREGARTEAN"
     gene            1645..2418
                     /locus_tag="VHM69_07360"
     CDS             1645..2418
                     /locus_tag="VHM69_07360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2740249.1"
                     /translation="MSDLSGKKALVTGAGMGIGQGIAVELARRGAEVAVHYAHTEPEE
                     TVSRIEKLGGKAATVQGDLSKVGECKRTVDAAAEALGGLDTLVNNAGVTKTLDFLDTD
                     EETYDLVFDLNMKGYFFLARRAVPFMMERDGGSIVNVTSVHGFAGFPNHTAYAATKGA
                     IGAFTRQLAIELTDRKVRVNAVGPGIIEVPRYFDIPNYTTEFGNTLVPWGRVGHPEDV
                     APAVAFLVSDAADFITGQILYIDGGTTARMGLWWDQGTE"
     gene            2501..>2778
                     /locus_tag="VHM69_07365"
     CDS             2501..>2778
                     /locus_tag="VHM69_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2740250.1"
                     /translation="MSERGSGINGLGDYRLGRRQFLAGSALASMGLLLAGCRREAAQQ
                     ANQGGGGGSGGNFPDTPEWNFVFVNHVTTNPFFVPTQYGIEDAEALLGT"
BASE COUNT          587 a          852 c          901 g          438 t
ORIGIN      
        1 tcttgcgcga cctcaaggcg aagggagagt tgccgcggga cctcgtactc aaggtctccg
       61 tgcagcttgc tgcggccaac gcggcggccg tcaaggcgat ggaggacctt ggggcgaata
      121 cgtacaacgt gcccacagac ctgtcgctcg cccaactcgc cgcgatacgc gccgctgccg
      181 agctaccgct cgacgtgtac gtcgaggtgc ctgacgactt cggcgggttc gtgaggcact
      241 atgagatccc cgacctcgta agggtggcat cgcccgtctt cgtcaagttc ggcctgagga
      301 acgcgccgaa tatctacccc agcggaacgc acctggaggc gacggcgatc gccctcggaa
      361 gggagagggt caggcgagcc aggatcgggc aggagatgct ggagcgctac taccccgagg
      421 ccaagacaac ggagaagggc gcgaccttcc ccggaatccc ggtcgagaca aaggagaggg
      481 catgaagata acgcacgtgg acacgttcgt cgtaggcacg gagtggcgca acctgaccat
      541 catccgcgcc cacaccgacg agggattgac cgggctcgga gaggcgcgga tggtcaacca
      601 caccgacgct ctggtggggt acattgccga agccgcaccg aggcacctcg tcgggcacga
      661 cccgttcagg atagaggatc tggtaaagaa gatgggccgc gacgacttct cgcgcgccgg
      721 agaggtgatg agcagcggca tcgccatgtt cgagacggcg tgctgggaca tcgtcggcaa
      781 agcgctcggc cagcctgtct acaacctgtt gggtggtccg gtcagagacc gcgtcaaagc
      841 ctacgccaac ggctggtaca gggtcgagcg cacgcccgag gagttccacg cagcggcgaa
      901 aaaggtcgtt gagcgtgggt acagggcgct caaattggat cctttcggtc cggggcatta
      961 cgagctcgag tatgaggaga agatcaaagc cgtctcgctc gtcgaggctg tcagggacgc
     1021 cattggccca gaggtggaga tcctgctcga gatgcacggc cgcttctcac ctgctaccgc
     1081 catagagatg gcccggatgc tccatccttt cggactcggc tgggtcgagg agccggtacc
     1141 tccggtcaat ctcaaagcat tgaagaaagc gtctgacggc atagaagcga ccgtcgcaac
     1201 gggcgagcgt atccaccagc gtctggagta cagggaggtc ttcgagcttc aggccgcgga
     1261 tgtgatccaa cccgacataa gccacatcgg cgggctcctg gagacgaaga agctcgcatc
     1321 gtgggccgac gcttactacg tgaccatagc cccgcacaac gttgggggcc agatcaacac
     1381 cgcagcagcc ctccacctgg ccgcctgtac gacgaacttc cgcatccagg aatacttcaa
     1441 cgacttcgcc gacccctggg tccgcgagac cgccccgggc ttgccggaag tcgtagacgg
     1501 ttacttcgag ctcccgaagg gacccggcct gggcgtcgag ttggatgaag aagtcatcga
     1561 ggcccacccc aagcagaacg tccacttcaa cctcttcgcc gagggctggg agagacgcga
     1621 aggcgcccgt acagaggcaa actaatgagc gacctctccg gcaagaaagc cctggtcacg
     1681 ggcgcaggca tgggcatagg tcagggcatc gccgtcgagc tcgcccgccg cggcgccgag
     1741 gtcgccgtcc actacgccca cacagaaccg gaagagaccg tctcgcggat agagaagctc
     1801 ggcggcaaag ccgccactgt gcagggcgac ctgagcaagg tcggagagtg caaacggacc
     1861 gtcgatgcgg ctgcggaggc ccttggcggg ctcgacacgc tcgtcaacaa cgcaggcgtc
     1921 acgaagacgc tggacttcct cgacaccgac gaggagacct acgatctcgt cttcgacctg
     1981 aacatgaaag gctacttctt cctggccagg agggccgtgc ccttcatgat ggagagggat
     2041 ggcgggagca tcgtcaacgt aacatcggtg cacgggttcg caggcttccc caatcacacc
     2101 gcctacgccg ccacgaaggg cgccataggc gccttcaccc gccagctcgc catcgagctc
     2161 acggaccgca aagtacgtgt caacgccgtc ggaccaggca tcatcgaagt tccccgttac
     2221 ttcgacatcc ccaactacac cacagagttc ggcaataccc tcgtcccctg gggccgggtt
     2281 gggcaccccg aagacgtcgc ccccgcggtc gccttcctcg tctccgacgc ggcggatttc
     2341 ataacaggcc agatcctcta tatagacggc ggtaccacgg cccgcatggg cctctggtgg
     2401 gatcagggga ccgagtaaat tctcttgaca agaatacata ttggtatgac aatgccgggt
     2461 gggaaaggca tctggaaaaa cgaatagaca ggggtaatag atgagcgagc gtggatccgg
     2521 cataaatggg ctcggggact acaggctggg ccgcaggcag ttcctcgcgg ggagcgcgct
     2581 ggcgtcgatg gggctgttac tggcggggtg caggcgcgag gcggcgcagc aggcgaacca
     2641 gggtggcgga ggcgggtctg gcggcaactt ccctgacacg ccggagtgga acttcgtctt
     2701 cgtcaaccac gtgacgacga acccgttctt cgtgccgacc cagtacggta tcgaggacgc
     2761 ggaggcgctt ttagggac
//
LOCUS       DATAIR010000302        10411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_147616, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000302 DATAIR010000000
VERSION     DATAIR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147616"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /gene="dnaE"
                     /locus_tag="VHM69_07370"
     CDS             complement(<1..1883)
                     /gene="dnaE"
                     /locus_tag="VHM69_07370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2740251.1"
                     /translation="MAVTSGGAFAHLHCHSEYSMLDGASRIKDLISFARDQGMPGLAL
                     TDHGVMYGAVKFYRAAKEAGIKPLMGCEVYVTADRNDRSRAPYYHLTLIARTAEGYRN
                     LMKLSTCGFLEGFYYKPRVDMEMLKKHGRGIICLSGCLSAEVPVRILEGRPDEARRLL
                     MEYEDIFDAVYLEMQDHGIEQQRRVNEGLIRLNKETGIPLVAANDSHYTTRNDAKMHD
                     VLLCIGTGKFYNDPKRMKFSGEEFYVKTVEEMARIFPDHPEALENTVKVVETVEDVGI
                     ELGKTRLPNFPKPEGYTADQYLREQCERGLIRRYGGRARSHEVRRRLEFELETIGKMG
                     FADYFLIVWDFVKYAKDRKIAVGPGRGSAAGSIVAYALEITDLDPLQYSLLFERFLNP
                     DRINMPDVDIDFSVSGRSEVMRYVTDKYGGHEHVAQIITFGTIGAKAAIRDSGRIYQY
                     PYSDTDKLAKFIPDKPVGTTLRDVLLPENGVYVPGEKHPGAAREIIQFVQQDEAAKQV
                     LDTAFEIEGFARHAGTHAAGVVISEEPLTDIVPLQAVKKASVKSDPMAGEEEEQLAVM
                     VQHPMSDVEALGLLKVDFLGLRNLDVIEESLETIRRGGGEEPDIRTIPLDDEKTLKLF
                     ARG"
     gene            complement(2201..2452)
                     /locus_tag="VHM69_07375"
     CDS             complement(2201..2452)
                     /locus_tag="VHM69_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740252.1"
                     /translation="MARGADLPQSGGRRDGLIRRSLRAVVLGVTLLFVVSALVVSCAL
                     VVYTAWRHDFSHGLFALLFAVLSAQIAMNWFLWKDEDDE"
     gene            complement(2457..2750)
                     /locus_tag="VHM69_07380"
     CDS             complement(2457..2750)
                     /locus_tag="VHM69_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX2740253.1"
                     /translation="MPIYEYKCENGHVFDVMRKLSEEPLTSCIECGAPVRKVLHPVGI
                     SFKGSGFYSTDYSSKGSTETAPSTTNGASKSGSGDEKSKEKPAGSSTSTGKKD"
     gene            complement(2818..3093)
                     /locus_tag="VHM69_07385"
     CDS             complement(2818..3093)
                     /locus_tag="VHM69_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740254.1"
                     /translation="MPEGEKRSFWKRLFATDRPSQREERVLEYIMHRIGEGAHLGDIV
                     EEEYVRRNASPQEIEEICARPELVQAAREHLQQDFESGELDPTRRPE"
     gene            3396..6521
                     /locus_tag="VHM69_07390"
     CDS             3396..6521
                     /locus_tag="VHM69_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2740255.1"
                     /translation="MKQEVVRLWLLGGFRMSVGSRAVASDRWRLTKAENLIKLLALAP
                     RHRLHREQVMDTLWPDIDAKHASNNLHRTLHFARGVLESEGADVGSDYLALRKDLLEL
                     CPDGPIWIDVEAFEKAAASARMSGAPASFRAAISLYAGELVPENLYEEWAYERRESLR
                     HLYKALLVELGALYEERDEYEKGIQTLRSAVAQEPTDEDAHAGLMRLYALSGRRPEAL
                     RQYERLRKTLTDKLDIHPTGASVRLYEQIRSGERPERHRADRPSAAPPPNNLPASLTS
                     FVGREDEVLEVKRSLAMTRVMTLTGAGGSGKTRLALEVARDLVGTYPEGVWLVELAAL
                     SDPTLVARAVAATLGVREQPDRSLTQTLRGHIGSGRMLLVLDNCEHLVDAAAWLAKDL
                     LGACPELRVLATSREPLRVSGEIVWPVPTLSLPDPETALTVDDLMGSEAVRLFVDRAR
                     SRLPAFELTRENARSVAAICLKLDGIPLAIELSTARMGALAVEQVAERLEDSLKLLTG
                     GDRTVAHRHQTLRATLDWSYELLSRPEQVLFRRLSVFAGGWTLDAAEVVGAGEGIQED
                     DVLDLLSQLVNKSMVVVDSADGSGLRYRMLEPVRRYGLERFGASGEADAVRRRHACWY
                     FELCKEVEPWLRGARHEVWLEQLEREYGNLRAALSWALERGEVDLGLWFGAALGESWY
                     MSGNLSEGRRWLEAALSNSGDAPPTPSRIKALLRAGWIAWEQGDYESSVAMSEESLML
                     SREFGDEAGTVAALSNLGWAALLVNDLQKASALAEEAVTLGSAMDDTGGIARALLIPG
                     LTAVAGGDQEQAIALHEESLALARKAGDDVAESLSLGMGVFAYLGQGDIRRAKALCEQ
                     SLALPPQPRVMNATSFQLHASAALASSQGLPLRSARLWGAAAALRENIGASLSPVELR
                     VYRPYIEAARQQVGETAWEEAWAEGKAMTVEQAEEYAVTDKEARARPRRVPREVVPGK
                     QSDDGLTGREWEVAHHVAAGLTNRQIASKLTISENTVANHVASIARKLNVPSRSRIAV
                     WVTQRELREVG"
     gene            6742..7350
                     /locus_tag="VHM69_07395"
     CDS             6742..7350
                     /locus_tag="VHM69_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740256.1"
                     /translation="MVKTIFSGAWQIGRATALALGVAVMLALVVGVASTAFGANGDDW
                     KLGRSNFATKITALGGALGVNGPMVRLTNNNAGTDDTALDLQVQAGEAPMTVNSSTRV
                     DSLNADQVDGKSASEFVASNIYKRESAVAAGTDKGDGTFVIGQACDPGDILLSGGPAN
                     VNGSSAMVESFPSPGTTNSWSARIQKNGLADNFSVVVLCADQ"
     gene            complement(7439..7921)
                     /locus_tag="VHM69_07400"
     CDS             complement(7439..7921)
                     /locus_tag="VHM69_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740257.1"
                     /translation="MTEVLVRSASSEDVPLIHSFIKELAEYERLSHEVFATEEMLSEQ
                     LFGERPVAGVLIAELGGDAAGFALFFHTFSTFLGKPGIYLEDLYVRPEFRGSGVGRAL
                     LDHLGRLAKERGCGRIEWAVLDWNEPAIGFYRSIGASPISGWTVYRVTGKALEELGEH
                     "
     gene            complement(7937..8893)
                     /locus_tag="VHM69_07405"
     CDS             complement(7937..8893)
                     /locus_tag="VHM69_07405"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX2740258.1"
                     /translation="MREGYTVAIVGAGLVGDRLVAELRRRNFPLKELRILARSARTAV
                     LAGETFEVGVAEPEAFEGVDIAFFAGTEGEKGAAVQLADEAISRGAVVIDNGSDFRLD
                     PKVPLVVPEVNAGALEGHEGLIANANCSTTQMVVTLAPLAREFGLGKVVVSTYQAVSG
                     AGRSGVEALENGREDAFPKPIVGNALPLIGGVGGDGNTTEETKMREESRKILSLPQLP
                     VFATAVRIPVHTGHAESIYVETEREVTLSEVLEALRSAPGVELPDDFPTPLEVAGEPG
                     THVGRVRVEGNVIQYWCVADNLLKGAATNAVQIAESLVSVPA"
     gene            complement(8895..9980)
                     /locus_tag="VHM69_07410"
     CDS             complement(8895..9980)
                     /locus_tag="VHM69_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740259.1"
                     /translation="MKRLEIVQLGLGHVGRAVAQIVLEERKRWLERRGIEVRYRAVSD
                     TSGALVGEDLLPRAIRLKEEGGRLAELGVEPLEGVLDSEPEPGYTRVVVDVAVHGGTF
                     DLDLLGIRGGAYLVLSNKGPLSGSTAQYLQLVRMIPDRLWHEATVGAGMPIISTIGGL
                     IETGDEVLEIQASPSGTLGFIMSAVESGRSFSEAVREAVALHYAEPDPRDDLSGLDVA
                     RKAIILARMMGREIEPEEIPYESLVPEGLEDVPLQEFMHRLPEHDEAFAERLLAVEEH
                     HMLRYLVRIPKEGRVTVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTLTVRGPGAG
                     PDRTASGVVWDLLDIAHKAVEDDEKDD"
     gene            complement(9977..>10411)
                     /locus_tag="VHM69_07415"
     CDS             complement(9977..>10411)
                     /locus_tag="VHM69_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740260.1"
                     /translation="GVFAGLGAKVLHPRTMEPASEAGIEVYVRNTFAPDGAGTHVTGF
                     DPGAGVRCVALRQNVAVQVPCSDGRAGRAAMVVCIGSPDEDDLANGRKVLRKAGIAPI
                     HFGIAAAGLVFVVSAEAGMDALRALHAGLIPPEGHFAGEEVA"
BASE COUNT         1893 a         3286 c         3332 g         1900 t
ORIGIN      
        1 tcgcccctgg cgaagagctt cagggtcttc tcgtcgtcga gaggaatcgt gcggatgtcc
       61 ggctcctcgc cgccgccgcg gcggatcgtt tcgagggact cttcgatcac atccaggttc
      121 ctgagtccga ggaagtccac cttgagcagc cccagcgcct ccacgtcgct catgggatgc
      181 tggaccatca cggcgagttg ctcctcttcc tcacccgcca taggatcgct cttcacgctg
      241 gctttcttca ccgcctgcaa aggaactatg tcagtgagcg gctcctcgga gatgacgacg
      301 ccggcggcgt gtgtccccgc gtggcgggcg aacccctcta tctcgaaggc ggtatcgagg
      361 acctgcttcg ccgcctcgtc ctgctggacg aactggatga tctcgcgcgc agcaccggga
      421 tgcttctccc cagggacgta gacaccgttc tccggaagca acacgtcccg cagcgtggtc
      481 cctacgggct tgtcggggat gaactttgca agtttgtccg tatcggagta gggatactgg
      541 tagatgcgcc ccgagtccct gatggccgcc ttagcgccga tcgtcccgaa cgtgatgatc
      601 tgggctacgt gctcgtggcc gccgtatttg tccgtgacgt agcgcatcac ctcggagcgc
      661 cccgagaccg agaagtctat gtccacgtca ggcatgttta tgcggtctgg attgaggaag
      721 cgctcgaaaa gcaacgagta ctgaagcggg tcgaggtccg tgatttccag ggcataagcg
      781 acgatggagc ccgccgccga gccgcgacct ggtccgacgg cgatcttcct gtccttggcg
      841 tacttgacga agtcccagac gatgaggaag taatcggcga agcccatctt accgatggtc
      901 tcgagctcga actccagcct gcgccgcact tcatggctcc tggcccgccc gccgtaacgc
      961 cggatcagac ccctctcaca ctgctctctg aggtactggt cggccgtata cccttcaggc
     1021 ttggggaagt tcggaaggcg cgtcttgccg agctctatcc cgacgtcctc gaccgtctcc
     1081 acgaccttga cggtgttctc cagcgcctcg ggatggtccg ggaagatcct ggccatctcc
     1141 tccactgtct tgacgtagaa ctcctcgcca gagaacttca tccgcttcgg atcgttgtag
     1201 aacttgcccg tcccgatgca aagcagcacg tcgtgcatct tcgcgtcgtt cctcgtcgtg
     1261 taatgtgaat cgttggcggc gaccagtggt atcccggtct ctttgttgag cctgatcaaa
     1321 ccctcattca cgcgccgctg ctgctcgatg ccgtggtcct gcatctccag atatacggcg
     1381 tcgaagatat cctcgtattc cataagcagc cttcgcgcct catccggacg cccctcgagt
     1441 atccgaacgg gcacctcggc agagaggcac cctgagaggc agatgatccc acgcccgtgc
     1501 ttcttcagca tctccatgtc cacccgaggc ttgtagtaga agccttcgag gaacccgcag
     1561 gtcgagagct tcatgaggtt cctgtagccc tcggcggtcc gcgcaatgag cgtgagatgg
     1621 tagtacggcg cgcggctgcg gtcgttgcgg tcggccgtga cgtagacctc gcaacccatg
     1681 aggggcttta tgcccgcttc ttttgccgcc ctgtaaaact tgaccgcgcc gtacatcacg
     1741 ccgtgatccg taagcgccag cccaggcatc ccctgatccc tggcaaatga gatgaggtct
     1801 ttgatgcggc tcgccccgtc gagcatcgag tactccgaat gacagtgcag gtgcgcaaac
     1861 gcgccgccgg acgtaaccgc caaactacct ccctcttctc ccgggtttcg gcgacgcccc
     1921 gatagagcgc cgccgttcgc ctgttctccc cgtcgatact cgacgcgggc tactatacgg
     1981 cactgacccg cccgttgtcc agagcacctc gaccacaagt tgagacctat tttgcaggac
     2041 ttttcaggga taggttacag gcttcaggta tcaggtttca ggttggagtt gggtggcgtt
     2101 gctacttttt gaagacgcat cgatgggtgc ttcttcaggc tatttgcggg tttcagagag
     2161 ttacgtgtgc agtgagaagc tgactgctga tacgtgaagt ctactcatcg tcctcgtcct
     2221 tccagaggaa ccagttcatg gctatctggg cgctcaggac ggcgaatagg agggcgaaga
     2281 ggccatggct aaagtcgtgg cgccaggcgg tatagacaac gagggcgcaa gagacgacga
     2341 gggccgagac gacgaacagg agtgtgacac cgagaactac ggcgcggagg gatcgccgga
     2401 tcaggccgtc ccggcggcct ccgctttgtg gcaggtcggc gccacgcgcc acctgctcag
     2461 tcctttttcc ccgtgctggt ggaggagccg gccggcttct ctttgctttt ctcgtcgccc
     2521 gaaccggact tcgaagcccc gttcgtggtg gaaggggctg tctccgtcga gcctttgctg
     2581 ctgtagtctg tcgagtagaa gcccgagccc ttgaacgaga taccgacagg gtggaggacc
     2641 ttccttacag gcgcgccgca ttcgatgcag gatgtcaggg gttcttcgga cagcttcctc
     2701 attacgtcga agacgtgtcc attctcacac ttgtattcgt agatcggcat ccagcctcca
     2761 gtttaaatgc tcgcgactcc agattataca ggctggatgg ctttctggtc cgcgcggcta
     2821 ctcgggacgg cgcgtcgggt cgagttcccc tgactcgaag tcctgttgga ggtgttcgcg
     2881 cgcagcctgt accagctcgg gtcgggcgca gatctcttct atctcctggg gagaagcgtt
     2941 ccgccggacg tactcttctt cgacgatgtc gccgaggtgc gcgccttcgc ctatccggtg
     3001 cattatgtat tcgagcaccc gctcctcacg ctgcgatggc cggtccgtag caaatagccg
     3061 tttccagaaa gatctcttct cgccctccgg catccttctc tccttgtcct ggatctcacc
     3121 cgaagtttat acgagtcgca ggctattcgt acagggcagg acaaggtgta cctccgcaga
     3181 taaccgttca tctccacgaa gcgcgccgtc accggaagat cttccggcac gagaaaatac
     3241 gtaatttgtg ggaggcgggt cacgaaattc gtatatatac tctgtaagta tacattgtaa
     3301 gttacgggtg agaggacacg ttcaccacgc aaatgaggag caataggggg aaggtgcacc
     3361 agccacacga ggcccgcacc cgcaggaata ttggcgtgaa acaggaagtt gtccggctgt
     3421 ggctgcttgg cggcttcaga atgtcggtcg ggtccagggc cgtcgcatcg gacaggtggc
     3481 gcctgacgaa ggccgagaac ctcatcaagc tactcgccct cgcgccccgg cacaggctgc
     3541 atcgggagca ggtcatggac actctatggc ccgatattga cgcgaagcac gcgtcaaaca
     3601 acctccatcg caccctccac ttcgcgcgcg gcgtgctcga aagcgaaggg gctgatgtcg
     3661 ggtcggacta tctcgcgctg cgcaaggacc tgctcgagtt gtgccccgac ggacccatct
     3721 ggatcgacgt cgaagccttc gagaaggcgg cggcaagcgc ccgcatgagc ggggctccgg
     3781 cgtccttcag ggctgctatc tctctctacg ctggcgagct cgtgccggag aacctctatg
     3841 aagagtgggc atacgagagg agggagagtc tgcggcactt gtacaaggcg ctgctggtag
     3901 agctgggcgc actgtacgaa gagcgtgacg agtatgagaa gggtatacaa acgctccgaa
     3961 gcgcggtcgc gcaggagccc accgacgaag atgcacacgc cgggctaatg cgcctgtacg
     4021 ccctctcagg gcggcggccg gaggcgctca ggcagtacga gcggctgcgc aagacgctca
     4081 ccgacaagct cgacatacat cccaccggcg ccagcgtgcg cctttacgag cagataaggt
     4141 caggggagcg cccggagcgt caccgtgcgg atcgcccgtc agcggctcct ccacccaaca
     4201 acctgccggc ctccctgacc agcttcgtcg gcagggaaga tgaggtactg gaggtcaaga
     4261 gatccctggc tatgaccagg gtcatgaccc tcacaggcgc cgggggctcg ggcaagacgc
     4321 gcctcgcgct ggaggtagcg cgcgacctgg tgggaaccta tccggaagga gtatggctcg
     4381 tcgagctggc ggcgctctct gatcccacgc tcgtggcgcg ggctgtggcg gcgactctgg
     4441 gggtgcgcga gcaacccgac cggtcgctca cacagacgct ccgcggtcac atcggatcgg
     4501 ggcgaatgct cctcgtgctg gacaactgcg agcacctcgt ggacgccgcc gcgtggttgg
     4561 cgaaagacct gctcggggcc tgtcctgagc tcagagtgct cgcgaccagc agggaaccct
     4621 tgagggtctc gggtgaaatc gtctggcccg tgcctaccct ctccctgccc gaccctgaaa
     4681 cggcactcac ggtcgatgac ctcatgggct cagaagcggt gcgcctcttc gtggaccgcg
     4741 cccgctcccg gctgccggcc ttcgagttga ccagggagaa cgcgcgctcc gtcgcggcga
     4801 tctgcctgaa gttggatggg ataccactcg caatagagct ctctaccgca cggatgggtg
     4861 cgctggccgt ggagcaggta gccgagaggc tcgaagactc cctcaagctc ctgacgggcg
     4921 gagatcggac ggtagcccac agacaccaga cgctccgcgc gacgctggac tggagctacg
     4981 agctgctcag taggccggag caagtattgt tccgccggct ctcggtgttt gccggcggct
     5041 ggacgctcga tgcggcggag gtcgtaggag cgggtgaggg catccaagaa gacgacgtgc
     5101 tcgacctcct ctcgcagctc gtgaacaagt caatggtcgt cgttgactcc gccgacggaa
     5161 gcgggttgcg gtacaggatg ctggagccgg ttcgccgcta cggcctggaa cgattcgggg
     5221 cgagcggtga ggccgatgcc gtccggcgca ggcacgcttg ctggtatttc gagctgtgca
     5281 aggaggtcga gccgtggttg aggggagcgc ggcatgaggt gtggctggag cagttggagc
     5341 gagagtacgg caacctgcgg gcggcgctga gctgggcact cgaaagagga gaggtggatc
     5401 tgggcctgtg gttcggcgcg gcgctgggag agtcctggta catgagcggg aacctgagcg
     5461 agggacgcag gtggctggaa gcggcgctgt ccaacagcgg cgatgcgcca cccactccat
     5521 caagaatcaa agcgcttctc cgcgcaggtt ggatcgcctg ggaacagggt gactacgaaa
     5581 gctccgtggc catgagcgag gaaagcctca tgctgtcgcg tgagttcggg gatgaggccg
     5641 gcaccgtcgc cgcgctctcc aacctgggct gggctgcatt gctggtgaat gatctccaga
     5701 aagcctcggc gcttgccgaa gaggccgtaa cactcgggag tgccatggac gataccggcg
     5761 gcatcgcccg cgcgctcctg atccctggcc tgacggcggt ggccggaggc gaccaggaac
     5821 aggccattgc actccacgaa gagagcctgg cgctggcgcg gaaagcggga gatgatgtag
     5881 cggagtccct ctcgctgggg atgggtgtat tcgcatacct cggccagggc gatatccgac
     5941 gagcgaaggc gctctgcgag caaagcctcg cgctgccgcc gcaacccagg gtgatgaacg
     6001 cgacctcgtt ccagttgcac gcatcggccg ccctggcaag ctcgcaaggg ctcccgctac
     6061 gctcggccag gctgtgggga gcggcggcgg cgttgcgcga gaacatcggt gcctccctct
     6121 cacccgtcga gctccgtgtg tacagaccct acatcgaagc cgcccgccag caagtaggtg
     6181 agaccgcgtg ggaagaggcc tgggccgaag ggaaggcgat gacggtcgaa caggccgagg
     6241 agtacgccgt caccgataaa gaagcgcggg ctcgtccccg tcgagttccc agggaagttg
     6301 tgccaggcaa gcaatctgac gacgggctaa ccgggcgcga gtgggaagtc gcgcaccacg
     6361 tcgcagcggg gctgaccaac cgccagatcg cctcgaagct gacgatttcc gagaacacgg
     6421 tcgccaacca cgtagccagt atcgccagaa aactcaacgt cccctccaga tcccgcatag
     6481 ccgtgtgggt gacccaacga gagctgcgcg aggtgggcta aagggtccct ttgggaccat
     6541 tccaggcaag agcaggcttc tcggaaaatc ttctgacgaa tagtcagaag taactacgcc
     6601 ggaacagcaa gaggtaagag cccggtaaga agcgctcgct acagtccggt caacaacaag
     6661 agccggagtc ggcgggcggc attctcccac gtcgatgagt ccggacggtc gaaagatccg
     6721 tcctggagaa aggaaggaac gatggtgaag acaatcttct cgggcgcatg gcaaatagga
     6781 agggctacgg ccctggctct gggcgtggct gtgatgctcg ccctggtcgt gggtgtggcc
     6841 tcgacggcct tcggggccaa cggcgatgac tggaagctgg gcaggagcaa cttcgccacc
     6901 aagataacgg cgctcggagg cgccctcggc gtgaacggtc cgatggtgcg cctgacgaac
     6961 aacaacgccg gcaccgacga caccgccctg gacctgcagg ttcaagcggg tgaggctccg
     7021 atgaccgtca actcgtccac aagggtcgac agcctcaacg cggatcaggt agacggcaag
     7081 agcgcctccg agttcgtcgc cagcaacatc tacaagcgcg agagtgcggt cgcggccggc
     7141 accgacaagg gtgacggcac cttcgtcatt ggccaggcgt gcgaccccgg ggacattctc
     7201 ctctccggcg gtccggcgaa cgtcaacggg tcgagcgcga tggtggagag cttcccttca
     7261 ccgggcacca ccaactcctg gagcgccagg atccagaaga acggcctggc ggacaacttc
     7321 tcggtcgtgg tgctctgcgc cgaccagtag gctgacagac agtctgtttt cgagcgggga
     7381 cctgggcttc cgggtccctg ttcctgatcg ccgaacgttc tcaggtagcg gatccagctc
     7441 agtgctcgcc cagttcctcc agtgccttcc cagtcacgcg gtagactgtc catccgctga
     7501 taggggaagc gccgatactt ctgtagaagc cgatggccgg ctcgttccag tccagtaccg
     7561 cccactcgat gcgtccacag ccgcgctcct tcgccagccg tccgaggtgg tccagtagcg
     7621 cgcggccaac acctgaccca cggaactcgg gacgcacgta gaggtcctcg aggtagatgc
     7681 ccggcttccc gaggaacgtc gagaatgtgt ggaagaatag ggcgaagccc gccgcatcgc
     7741 caccgagttc tgcgatcagc accccggcaa cgggacgctc gccgaagagc tgctcgctca
     7801 acatttcctc ggtcgcgaag acttcgtgcg agagacgctc gtactcggcg agttccttga
     7861 tgaaagagtg gatcaggggc acgtcctcag aggaagcact acgtacgagg acctcggtca
     7921 tctgatctct attcgcttac gccggcacgc tgaccagtga ctcggcgatc tggaccgcgt
     7981 tcgtcgccgc gcccttgagc aggttgtcgg cgacgcacca gtactggatc acgttccctt
     8041 ccacgcgcac cctcccgacg tgggtccccg gctctccagc gacctcgagc ggcgtcggga
     8101 agtcgtccgg caactcgacg cccggcgcag atctgagggc ttcaagcacc tcggagagcg
     8161 taacctctcg ctccgtctcg acgtagatgc tctcggcgtg gccagtgtgg acagggatgc
     8221 gtacggcggt cgcgaagacc ggaagttggg gtagagagag gatcttgcgt gactcctccc
     8281 gcatcttggt ctcctcggtc gtgttgccgt cccccccgac acccccgatc aagggtagag
     8341 cgttcccgac tatgggcttg gggaaggcgt cctccctgcc gttctccagc gcctcgacgc
     8401 cgctgcgtcc cgcccccgag acagcctggt acgtcgagac gacgaccttg ccgaggccga
     8461 actcgcgcgc gagcggggcc agggtgacga ccatctgggt ggtcgagcag ttggcgttgg
     8521 cgattagacc ttcgtgacct tcgagcgccc ctgcattcac ctcggggacg acgaggggca
     8581 ccttcgggtc gagcctgaag tcgctgccgt tgtctatgac gacggcgcca cgggagatgg
     8641 cctcgtcggc gagctggacg gcggccccct tctcaccttc tgtaccggcg aagaaggcga
     8701 tgtcaacgcc ttcgaaggcc tcgggttctg ccacaccgac ctcgaaggtc tcccctgcga
     8761 ggaccgcggt gcgggcgctc cgggcgagga tccgcaactc cttcagcggg aagttcctgc
     8821 gcctcaactc ggcgacgagc ctgtcgccta cgagtccggc gcctacgatc gctacggtgt
     8881 atccctcacg catgtcagtc gtccttttcg tcgtcctcta ctgctttgtg cgcgatatcc
     8941 agaaggtccc agacgacgcc gctcgcggtc cgatccgggc cggctcctgg gccgcgcacg
     9001 gtgagcgtaa catccgagta gcggcgcgtg cggaagtcga agacgttctc ggggccgcta
     9061 atagggccga aggggctctc cacgggtgtg tcctgcaggc cgaccgtgac gcggccctcc
     9121 ttcgggatgc ggaccaggta gcggagcatg tggtgttcct cgacggcgag aaggcgctcc
     9181 gcgaaagcct cgtcgtgctc ggggaggcgg tgcatgaact cttgcagagg gacgtcctcc
     9241 aggccctcgg ggaccagtga ctcgtagggg atctcctcag gttcgatctc ccgccccatc
     9301 atgcgcgcga ggatgattgc tttgcgggcc acgtccagcc ccgagaggtc gtcgcggggg
     9361 tcaggctccg cgtagtggag cgcgacagcc tccctcaccg cctcggagaa ggagcggccg
     9421 gactcgaccg cgctcatgat gaagccaagc gtgccgctcg ggctcgcctg tatctcgagc
     9481 acctcgtcgc ccgtctcgat gaggccgcct atcgtcgaga tgatgggcat gccggcccct
     9541 accgtcgcct cgtgccacag acggtcgggg atcatgcgca cgagctgcag gtactgggcc
     9601 gtgctccctg agagcggtcc cttgttggag agcaccagat aggcccctcc acgtataccg
     9661 agcaggtcga ggtcgaaggt tccgccgtgg accgccacgt cgacgaccac acgcgtgtag
     9721 ccgggctcgg gctccgagtc gagaacgcct tccagcggct cgacaccgag ttcggcgagc
     9781 cttccgcctt cctccttgag gcgaatcgcc cgcggcagga gatcttcccc cacgagcgcg
     9841 ccggaggtgt ccgagaccgc acgatacctc acctcgatac cacggcgctc caaccagcgt
     9901 ttgcgctctt ccaggactat ctgggcgacc gcgcgtccaa catggccaag gccgagttgg
     9961 actatctcca gtcgcttcac gcgacctctt cgccggcgaa gtgtccttct ggtgggatca
    10021 ggcccgcgtg caacgcgcgt agtgcgtcca tgcccgcctc ggcggacacg acgaacacca
    10081 gccctgccgc cgcgatgccg aagtggatcg gcgctatgcc cgcctttcgc agaaccttcc
    10141 gcccattggc gaggtcatcc tcgtcgggcg agccgatgca gactaccatc gccgcacgac
    10201 cggcgcgacc gtccgaacag ggcacctgga cggcgacgtt ctgccgcagg gcgacgcagc
    10261 ggacgccggc acccggatcg aagcccgtaa cgtgggtgcc agcgccatcg ggcgcgaacg
    10321 tgttgcggac atagacctcg ataccggcct cggaggcggg ctccatcgtc ctcgggtgca
    10381 ggaccttcgc gccgaggccg gcgaagacgc c
//
LOCUS       DATAIR010000303         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_388383, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000303 DATAIR010000000
VERSION     DATAIR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388383"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VHM69_07420"
     CDS             <1..1559
                     /locus_tag="VHM69_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740261.1"
                     /translation="WLLEQAALSRVSGLDTIELVLRYLTPTLVVVALLMVYALARTLL
                     KNERAAVLTASGYALFHIIFIQPSVQNVGVELAARISEDKYAARFLILPATLLCAALY
                     IESRKWRYLGLFTFFCWAAIAVHPSVLPAIGLCMLGFGVAHVAANLRGRAAWTGMVAL
                     ALAMWSVVLGPALLLFAGRSPAAVLYSADINSTPPKVLEYTVFITESWRHIYELGAGY
                     YIMHPWLLVNPVILGTYVFGVPFLLWRVRTSVAAQFLLGGLAVVSVAVYVPPIATFIG
                     NDLIVPGLLWRLAWPIPLLALLTAGWMVWEALGYAEARLSGLGMQRGVTRVLPLALVV
                     LLTAAAAPPSVEKAVGLYRKFDVARTTDYHPDPIYPWIRDNMNEPGVLLARDSANNAV
                     PAYSASLNVVSQRGEGMIRDREKLEKLAGSEIDIPRRYLDVHSFFFGPTLDEEAYDIL
                     RRYQIDYLMVYAGSPLDERLKSLPGFSHMEDAPRYKYSLYTVDLRKLGEPARGSAKPR
                     ETAPPAVERR"
     gene            1612..3024
                     /locus_tag="VHM69_07425"
     CDS             1612..3024
                     /locus_tag="VHM69_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX2740262.1"
                     /translation="MSPAFDPRLSAAILAARSAQFASRTLKTGGGSTVPGVVARRVDP
                     MVLTKLSRRLRLHSAAVTGTNGKTTTTRMVLKVLQAAGVRAVNNSTGANLVTGVTAAL
                     VSDSGFSGEPSSEMGLFEVDEASVPRVAAEAEIKILAVLNLFRDQLDRYGELAYTGKV
                     IASAFGDLPPDGAVVLNADDPLVASLGRSAKRPIFYGVDEPALDTGRLQHVADSKDCP
                     VCGTPLVYETVYMGHVGVYDCRHCDFSRPEPAYRASRVRLHGARGAEFLLSTPKGGAA
                     VRIGLPGLYNVYNALAAAAVAGESGIGLPEISRGLREFGGAFGRVERVKAGDREAFLL
                     LIKNPVGFNEILRTFVTGADAKNVLIAINDNDADGRDVSWLWDVDFEMLAEAKAEGKS
                     ESAPFTVSGTRAGDMAVRLKYAELPVGPVIPDREEAIKAALEATPPGETLYVLPTYTA
                     MLEIRKTLSEMGYTHPFWED"
     gene            3282..4421
                     /locus_tag="VHM69_07430"
     CDS             3282..4421
                     /locus_tag="VHM69_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2740263.1"
                     /translation="MNQRIHGEKREKPEDVVRWLGAVQAQDYMQALWAIGLRSTSATV
                     ADVEQAILDGKIVRTWPMRGTLHFVPPEDAKWMLKLSASRMLAKDGRRLKQLGLDEEI
                     MERCKEIFYEALKGNRRLSRQDMMGLLEDAGISTENQRGYHIMWYVSQGGLICLGPMR
                     DKQQTFVLLDEWVPDSKSLSREESLTELASRYFASHGPATVHDLAWWAGLTVTDARAG
                     LEAAKPGLLSETLDGKEYWMTDDSQADLAFDESNVSLLPGFDEYLLGYRDRSAVLAVE
                     HAPRVVPGRNGVFRPIIVVGGLVIGTWKRRLKTNAIDMTLSLFTHTDDSQKRVIEAAR
                     SYSEFLGLPLSSTRVEEGTRRQWIPSKSGGAPYASHAPTVNIAGS"
BASE COUNT          866 a         1315 c         1419 g          865 t
ORIGIN      
        1 ggtggttgct cgagcaggcg gccctctccc gcgtctccgg cctggacacc atcgaacttg
       61 tgctcagata cctgaccccg acgctcgttg ttgtagccct gttgatggtc tacgccctgg
      121 caaggaccct attgaagaac gagcgggccg ccgtcctcac agcctcgggc tacgccctgt
      181 ttcacatcat cttcatccaa ccatccgtac agaacgttgg ggtggagctg gcagcccgta
      241 tctcggagga caagtacgca gcaaggttcc tgatactacc cgcgacgcta ctctgcgccg
      301 cgctctacat agagagccga aaatggagat acctcggcct cttcacgttc ttctgctggg
      361 ctgcgatagc cgtacatcct tctgtgctgc ctgccatcgg gctgtgcatg ctcggtttcg
      421 gggtggcgca cgtggccgca aatctacgcg ggagagccgc ctggacgggt atggtcgccc
      481 tcgcgctcgc gatgtggagc gtcgtcctcg ggcccgcgct gttgctgttt gcgggcaggt
      541 ctccagccgc cgtgctgtac tccgctgata tcaactcgac gcctccgaaa gttctcgaat
      601 acaccgtctt cattacagag agctggaggc atatctacga gctaggcgcc ggctactaca
      661 taatgcaccc atggttgttg gtgaacccgg tgatcctggg tacttacgtg ttcggcgtcc
      721 ctttcctgtt gtggcgtgtg agaacgagtg tcgcggccca gttccttctc ggagggctgg
      781 ctgtcgtgtc agtcgccgtc tacgttcctc cgatcgccac ctttatcggg aacgacctca
      841 tcgtccccgg tctcctgtgg cgtctcgcgt ggcccatacc gctcctcgcg ctactgacgg
      901 ccggctggat ggtgtgggag gcgttgggtt acgccgaggc gcgcctgagt gggctcggta
      961 tgcagcgcgg tgttacaagg gtcctgccgc ttgcgctcgt ggtccttctg acggccgccg
     1021 ccgcgccgcc ttccgtcgag aaggccgttg gactgtaccg caagttcgac gtcgcaagga
     1081 ccaccgacta ccacccggat cccatctacc cctggatcag ggacaacatg aatgagcctg
     1141 gcgtcctgct cgcgcgcgat agcgcgaaca acgccgtccc cgcgtactcg gcctcgctca
     1201 acgtggtgag ccagcgggga gagggtatga tccgggaccg cgaaaagctg gagaagctgg
     1261 cagggtccga aatagacatc ccccgacgct acctcgacgt acacagcttc ttcttcggac
     1321 ccacgctgga cgaagaagcc tacgacatac tgcgccgcta ccaaatagat tacctgatgg
     1381 tctacgctgg cagcccgctc gacgagcggc tgaagtccct gcctggattc tcacacatgg
     1441 aggatgcccc cagatataag tacagtctct acacggtgga cctccggaaa ctaggcgagc
     1501 cggcccgtgg ttccgccaaa cctcgcgaga ccgcgccgcc ggcagtcgag cgtcgctagg
     1561 agcaacgagc ctctcccgca agatcacacc cggacccata taatccgcac tatgagtcct
     1621 gcgttcgatc cgcgtctatc ggcggctata ctggccgccc ggtctgcaca gttcgcgagc
     1681 cggacgctga agaccggcgg gggctcgacg gtgcccggtg tcgtggcacg cagggtggac
     1741 ccgatggtcc tgacaaagct ctcgcggcgg ctgcgcctgc attcggcggc cgtcacgggc
     1801 accaacggca agactactac gaccaggatg gtattgaagg ttctccaggc cgccggtgtc
     1861 agggccgtga acaactcgac gggggcgaac ctggttaccg gcgtgaccgc ggctctggtc
     1921 tcggattcgg gcttctccgg ggagccgtct tcggagatgg ggctcttcga ggtggacgag
     1981 gcgagcgtcc cgagggtggc cgctgaggct gagatcaaaa tcctcgccgt ccttaacctg
     2041 ttcagggacc agctcgaccg ctacggggaa ctcgcctata cgggcaaggt catcgcctcg
     2101 gctttcggag acctgccgcc agatggcgcc gtggtcctga acgcggacga ccccctcgtc
     2161 gcgagcctcg ggcgctcggc gaaaagaccc atcttctacg gggtcgacga gccggccctg
     2221 gatacagggc gcctgcagca cgtagctgac tcgaaggact gtcctgtgtg cgggacgccc
     2281 ctcgtctacg agaccgtgta catggggcac gtcggagtct acgattgccg tcattgcgat
     2341 ttctcgaggc cagagccggc ataccgcgcg agccgggtca ggctgcacgg ggcgcggggt
     2401 gccgagttcc tcctatcgac gccgaagggc ggggcggccg tgaggatagg gcttcccggc
     2461 ctgtacaacg tctacaacgc ccttgccgcc gccgccgtgg ccggggagtc agggatcggg
     2521 cttcctgaga tctcgcgcgg cctgagagag ttcggggggg ctttcgggag ggtcgagcgg
     2581 gtcaaggcgg gggacaggga ggccttcttg ctgctcatca agaaccccgt ggggttcaac
     2641 gagatcctgc gcaccttcgt caccggcgcc gacgcaaaga acgtcctcat cgccataaac
     2701 gacaacgatg ccgacggccg cgacgtgtca tggctctggg acgtggactt cgagatgctc
     2761 gccgaagcga aggcggaagg caagtccgag agcgccccct tcaccgtcag cggcaccaga
     2821 gccggggaca tggcagtgcg gctcaagtac gcggagcttc ccgttggccc cgttatccca
     2881 gaccgcgaag aggccataaa ggccgcgcta gaggcgaccc cgccaggcga gacgctctac
     2941 gtgctcccca cctacacggc catgctggag atccggaaga ccctgagcga gatgggctat
     3001 acgcatccgt tctgggaaga ttgacccgca gacttcggcg cggacgacgc tttcaccttc
     3061 gtccaggtag gcttggagaa atacagatcg aggatgggag cgccgcgtgc cacccgtaga
     3121 atctattggc gggtggtcaa agaaaggcgg cgtgtgcaaa tggatgtagt ctctatgccc
     3181 gaagccgggc gagtcggtgg gccgttgcgg tgttctgtcg gcgcagcaaa ctgttgtctg
     3241 gagtaggagt gataactacg gacatcgtca ggcgaaggct gatgaaccag cgcatccacg
     3301 gtgagaaacg tgagaagccc gaagatgtgg tgagatggct gggcgctgtt caggcgcaag
     3361 attacatgca ggcattgtgg gcgatcggcc tgcgctcgac atccgcgacg gtggcggatg
     3421 tcgagcaagc catactcgac gggaagatcg tccggacatg gccgatgcgt ggcacgcttc
     3481 atttcgtgcc gccggaagat gccaagtgga tgctgaagct ttcagcgtcg cgcatgcttg
     3541 ccaaggatgg gcgaaggctg aagcaactgg gactcgatga agagatcatg gaacgttgta
     3601 aggagatctt ttacgaagca ctcaaaggca acaggagact atcccgccag gacatgatgg
     3661 ggttgttgga ggacgcaggt atcagtaccg agaaccagcg cggttatcat attatgtggt
     3721 acgtatccca gggtggcctg atctgtctgg ggccgatgcg agacaagcag caaacattcg
     3781 tgctgttgga cgagtgggtg cccgattcca agagtttgtc tcgggaggag tcgctcaccg
     3841 aactagccag tcgctacttt gccagccatg ggccggcaac ggttcatgat ctcgcctggt
     3901 gggcgggatt gacggtcacc gatgctagag caggacttga ggcggcaaag cctggactct
     3961 tgtcagagac tctggatggc aaagaatact ggatgacaga tgattcgcag gccgatttgg
     4021 cgtttgacga gtctaacgtc agcctactcc cgggtttcga tgaatatctg ctcggctaca
     4081 gagaccgtag tgccgtgctc gcggtcgaac atgcgcctag agtcgtcccg ggcagaaacg
     4141 gcgtctttcg accgattatc gtcgttggtg gactagtcat tggtacctgg aaacgacgct
     4201 tgaagacgaa cgctatagac atgacattga gcctgttcac acacactgat gattcgcaga
     4261 agcgagtcat cgaggctgct agatcttata gtgaatttct tggcttgccg ttgtcgtcaa
     4321 cacgagtaga ggaggggact aggcgccaat ggattccatc aaagtctggc ggagctcctt
     4381 atgcaagcca cgctccaaca gtgaacatcg ccggatcatg aaaaaatcag gagggaacgg
     4441 aaactatgcc gttccctccc atgag
//
LOCUS       DATAIR010000304         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_147704, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000304 DATAIR010000000
VERSION     DATAIR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147704"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VHM69_07435"
     CDS             <1..550
                     /locus_tag="VHM69_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2740264.1"
                     /translation="IARALSYAARNDADVINLSLGSRSRSTLLQQAIRDATDKGVLVT
                     AAAGNSNSALPHYPAAGNGAAASSDGLVSVTSVDMYGKKSNFANYGTWVDLAAPGDAI
                     RSAFPNDRYAYWSGTSMATPFVSGEAALIHAVYGSFDPVSVEGKIRCSTQSLLTSDLI
                     YSAVLGTGRADVGASLAPGVCS"
     gene            791..1261
                     /locus_tag="VHM69_07440"
     CDS             791..1261
                     /locus_tag="VHM69_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740265.1"
                     /translation="MLDRYERLVYSVPRRYRLSPEDAADITQLTFTILIQSMETLSED
                     SNLGAWLITVARRHSWRLLERNRRKVAGEYAIRGETNTLPASEEIDRWERSEWLNHGL
                     SLVSRDCRELLSALYLEPEQPSYVDVAARLGMAVGSVGPTRIRCLKRLRQALGE"
     gene            1383..2009
                     /locus_tag="VHM69_07445"
     CDS             1383..2009
                     /locus_tag="VHM69_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740266.1"
                     /translation="MAVPKKIDFSLLVDWVEGRLSAEETRGVEERLATADSETLSDIA
                     WLRKFGAATQEAALESPPAGVRSALVARFVAYAEGRRTPGFLERAVATLTFDSGLRPA
                     VGARSAGGQSTRRQLIYSVDALDIAINFWPRPRDKNLDLEGQILPRDGLEPESFSVQM
                     LQDETELAMTTTDELGGFSFESIMPGVYTAIFSTDQIEVSLDPVDLTA"
     gene            2061..>3832
                     /locus_tag="VHM69_07450"
     CDS             2061..>3832
                     /locus_tag="VHM69_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740267.1"
                     /translation="MLAAPTLEEISSVLRSANLLNPDGLERLLDFADRLIYSDPGKAQ
                     RLATVCTELADRAAAPSAVPHALYIRAQAHAENGAFAAALRMAETARQGYLACGKNLE
                     ALRTHVGRMSVLLDLGRYQEALDSGQAVLDTLDGKGEFEVTPTRQQASLLTALVHQNR
                     GVCYEYVGRYEEALEAYEVAEVLYRTLGMIERIGEILDNRGAILLYLGRGHEALAAHQ
                     AAAVVFADAGLNFSRARALSNIGEAHRQLANYMQSLEAFEQARHLYDSLDALADKGLL
                     MVDTAGAYQELNLYAEALASYREAHALLSNVGMAHDRARASWGMGSALSARWEFEEAE
                     RALVEAADLFAAADNAPLLSGVMLEQAALQEARGDHGAAVTTAGRALDLVSAGDWPAQ
                     RVYAHLRLADLLPDEAEAHLLEAQKLAERLALPHLRYRLYERLGRLRRLQDRDEEARG
                     LLEAAVDDIERLRGTVAHETLRASFLRDKTAAYEELLKLYLARGTREDSCRAFAVAER
                     AKSRALADLLTGVAGEPAMIADDALEGRIRDLQSELNVAYTQMLGGTEEPGIPLPDLR
                     GRAAELEAEISRLQLRATVVHDPFS"
BASE COUNT          722 a         1213 c         1215 g          682 t
ORIGIN      
        1 gatagcccgg gcgctctcct acgccgcgcg caacgacgct gacgtgatca acctctccct
       61 cggctcaagg agcaggtcta cgttgctcca gcaggcgatc agagacgcca ccgataaagg
      121 tgtccttgtc accgccgccg caggcaactc caacagcgcc ttgccacact accctgctgc
      181 cggaaacggt gccgcagcct cttctgatgg gctcgtgtcc gtaacttctg tagacatgta
      241 cggcaagaaa tccaacttcg ccaactacgg aacctgggtg gacctcgccg cgcccggcga
      301 tgccatcagg agcgcgttcc cgaatgaccg ctacgcttac tggagcggca cctcgatggc
      361 gacgccgttc gtctccggcg aggcagccct catccacgcg gtctacggct ccttcgatcc
      421 tgtgagcgtg gaagggaaga tacgttgcag cacgcagtcg ctcctcacgt cagacttgat
      481 ctacagtgcc gtgctcggca ccggacgcgc cgacgtaggc gcgagcctcg ccccaggcgt
      541 ctgctcatag aaaccgggga gtcgggcagc ctccgcccgc cctgtcagac gatgaccggt
      601 accgccgggt tcatcgtcga acgcgctcaa acccgcgacc gctcgccaac cacgaggggc
      661 ccgtccggcg ttgtcttgca tgtagggtac ggccatgctc tccgtccgga agcacatgcg
      721 aagtgagcca agatcagacc gaagcctgat ccgggcctgt cgcaggggcg acgttggcgc
      781 ctggcggaac ttgcttgaca gatatgagcg gctcgtttac tccgtaccgc gaagatacag
      841 gctctccccc gaggatgccg cggatatcac acagcttacg ttcaccatct tgatccagag
      901 catggaaacg ctgtcggagg acagcaacct tggcgcgtgg ctgatcaccg tggcccgccg
      961 ccactcctgg cgtctgcttg agcgcaaccg tcgtaaggta gcgggcgagt acgcgattcg
     1021 gggcgagacc aacacgcttc cggcaagcga ggaaatagat cgctgggagc gtagcgagtg
     1081 gttgaaccat ggcttatctc ttgtgagcag ggattgccgc gagcttctat ccgcactcta
     1141 tttggaaccg gagcaaccat cttacgttga tgtcgccgcg cgtctgggta tggccgtagg
     1201 tagcgtgggc ccgacacgga tccgctgcct gaagcgtttg aggcaggcac ttggtgagta
     1261 gtatgggcca ccatcgctgc gcagggctct cgcaccggtt caggtcggtg tatttcttgg
     1321 tgtcgtggta agcatgtaca gggtaagatg aattgcgaag gagcgccgga ggtggtcaga
     1381 ctgtggccgt acccaagaag atagatttct ccctgctcgt cgactgggta gaagggcgtc
     1441 tgtccgcgga ggagacacgc ggcgtcgaag agcgattggc gactgccgac agcgagacgc
     1501 tctctgatat cgcctggctg cgcaagttcg gcgcggccac ccaagaagcc gcccttgagt
     1561 cgccgcctgc cggggtaagg agtgcgctgg tcgcccgctt cgtggcctat gcggagggcc
     1621 gtcgtacgcc cggtttcctc gaacgggctg tcgcgacgct gaccttcgac agcggcctgc
     1681 gtcctgcggt cggcgcgagg tcagccggcg ggcaaagtac aaggaggcag ctcatctact
     1741 ccgtggacgc cctggatata gccattaact tctggccgcg cccccgcgac aagaacctcg
     1801 acctcgaggg ccagatcctt ccccgcgacg gcctggagcc cgaatccttc agcgtccaga
     1861 tgttgcagga cgaaaccgag ctggccatga ccactacgga cgagctgggc ggattctcgt
     1921 tcgagtctat aatgcccggc gtgtacacag cgatcttcag cacagaccag atcgaggttt
     1981 cgctcgaccc cgtcgatctg accgcctagc gcgcgacttt ggtctccggg cagagtgccg
     2041 ttgccggata cgtcgaggag gtgctcgcag caccgacgct ggaggagatc tcgtctgtat
     2101 tgcgctccgc gaacctgctc aatcccgacg gccttgagcg gctgctcgac ttcgccgacc
     2161 gcttgatcta cagcgatcct ggcaaggcgc aacggttggc aactgtctgc acggagctgg
     2221 cggaccgggc agccgcgccg tccgcagtac cccatgcttt atacattcgt gcacaggcgc
     2281 acgcggagaa cggcgcgttc gctgctgcac ttcgcatggc cgaaaccgcc cgccaggggt
     2341 atctcgcctg cggcaagaac ctcgaggcac tgcgtaccca cgttggccgg atgtccgttc
     2401 tcttggacct tggacgctat caggaggcac tcgattccgg gcaagccgtt ctcgacaccc
     2461 tcgatggaaa gggcgaattt gaggttacac ctacaaggca gcaagccagc ctgctgaccg
     2521 cgctcgtgca ccagaaccgc ggcgtttgct acgaatatgt aggccgttac gaagaggcac
     2581 tcgaggcgta cgaggtcgca gaagtgcttt accggaccct tggcatgatc gaacgcatcg
     2641 gcgagatcct cgacaataga ggcgccatac tactgtacct cggtcgtgga cacgaggcgc
     2701 ttgctgcaca tcaggcggcc gcggttgtct tcgccgatgc gggacttaat ttctctcgtg
     2761 ccagagcgct tagtaacatc ggggaagcgc accgtcagct tgcgaactac atgcagagcc
     2821 tggaagcttt cgaacaagcg cgtcacctgt acgactcgct cgacgcgctc gccgacaaag
     2881 gcttgctcat ggtcgatacg gcaggggctt accaggagct caacctgtac gccgaggcgc
     2941 tggcgagcta cagggaggcc cacgccctgt tgagcaacgt cggtatggcg catgaccgtg
     3001 cgcgggcatc atggggaatg ggctcggcgc tgagcgcaag gtgggagttc gaggaggccg
     3061 agcgtgcgct ggtcgaagcg gccgacctct tcgcggcggc cgacaacgcg cccctgctct
     3121 cgggcgtgat gctggaacag gccgccctgc aggaggcgcg aggggatcat ggagcggcgg
     3181 taactaccgc gggtcgggcg ctggatctcg tctccgcggg agattggccg gcgcaacgcg
     3241 tctacgccca cctgcggctc gccgacctgc tgcccgacga agcagaagct cacctcctgg
     3301 aggcacagaa gcttgccgaa cgccttgcgc tgccccacct gcgctaccga ttgtacgagc
     3361 ggctaggtcg cctgcggcgt ctgcaagacc gcgacgaaga ggcgcgcggc ctgctcgaag
     3421 cagctgtgga cgacatcgaa cgcctgcggg gtaccgtcgc ccacgagacg ctgcgtgcct
     3481 cctttctacg agacaagacc gccgcctacg aggaactgct gaaactctac ctcgcaaggg
     3541 gaacccgaga ggactcgtgc cgtgcctttg ccgtcgccga aagggccaaa tccagagccc
     3601 tggcggatct gctcaccgga gtggccggag aaccggctat gatcgcagac gacgcgctgg
     3661 aaggccgaat ccgggatctt cagtcggagc taaacgtggc ttacacacag atgctcggag
     3721 gcaccgagga acccggcatc cctctaccgg acttgcgcgg gcgggcggcc gagctcgaag
     3781 cagagatcag ccgactgcag ctgcgcgcta ctgttgtgca tgacccgttc tc
//
LOCUS       DATAIR010000305         8827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_331738, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000305 DATAIR010000000
VERSION     DATAIR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331738"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VHM69_07455"
     CDS             complement(<1..527)
                     /locus_tag="VHM69_07455"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740268.1"
                     /translation="MSTTSATPEADKRTSNREQRGWYFYDWANSAFSTTVLTVFLGPY
                     LIAVTENAADTGGYVHPLGIPILAGSFFPYVLSLSVVLQVFLLPVTGAIADRTQRKKR
                     LLALLAYIGAISTMGLYLIQGSAYLFGGLLFVIANLSFGASIVVYNAFLPEIAEPDER
                     DRVSSRAWALGYLGGG"
     gene            complement(653..3409)
                     /locus_tag="VHM69_07460"
     CDS             complement(653..3409)
                     /locus_tag="VHM69_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2740269.1"
                     /translation="MDDRNEGTGLVMSEERTLRWLVDTLSWRGGRMAVVALREEGVER
                     WSYLELSDNVRRLGRGLRWAGVERGDHVAIFAGNRPEWVVACLAVIHAGAIVVPLDAQ
                     LDDAALGRIIDDSGTRFIFTTADRAERVERLDLDDGPRSILLDAGEGDERSWRLLLAD
                     DEVELPRVEPGDPAALFYTSGTTGTAKGVPLSHRNLVFQIDALLGADLVSEDDSILLP
                     LPLHHVYPFVMGMLTPLAAEVPIVMPQSLTGPQLVRALQEGEVTLIIGVPRLYGALYS
                     GIEERIKSSGRVAAALFGAGIQFCVRLRRRTGFDVGKVPLRPLRERFGPRLRVLASGG
                     AALDPDLAWKLEGMGWRVAVGYGLTETAPLLTLNPPDGEKLGSVGRPVPGVEISVDPS
                     AVPDESQRRLDGKLRREGEILARGPNVFSGYRNRPEETSKVFTDDGWFRTGDLGYFDD
                     DGYLYVTGRASTLIVTASGKNIQPEDVEGAYQENPYIREVGVLQKDGRLVALIVPDIV
                     QIRRLGDGDIQQSIREAVEEGSKRLPSYQRISDYAVSREPLEYTRLGKLRRHVLDERY
                     ERAKRGKESQDEAATGPIAVEEMSEADRVLMGNPAAATVWDLLANRYPDRGLTPESSP
                     QLDLDIDSLGWINLTLEIAEKAGVDLDEEAIQHIDTVRDLLREVAREAEAGGTEVHTS
                     PLARPEEVLNDRQKRWLAPLGPAASALAGGMFTLNRMLMRGPFRLRVEGLENLPDEGP
                     FIAAPNHVSYLDSFAVAAALDDRVLRRTYWAGWTGAAFGNPLTRLVSRLAQVVPVDPD
                     RAGLSSLAFGAAVLKRGRNLVWFAEGERSPTGRLQQFKPGMGILLNYYSVPVVPVFIS
                     GTYEAMPRGKFLRRLEQVTVTFGEPLVAGDLETASSQQEVVQAVRERVAKLANTSDSR
                     RDAS"
     gene            3641..4405
                     /locus_tag="VHM69_07465"
     CDS             3641..4405
                     /locus_tag="VHM69_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX2740270.1"
                     /translation="MSVRGVVLDLDGTLYVGGEPVPGARDAVERLKASGLRLRYVTNT
                     TRMPRRAVVRSLRTLGFQVAEGEVFTPAWAAARLIGDGACLPLVDDALREDLDGVRFT
                     EDSPEFVLVGDLGEGFTYARLDKAFRRLMDGAELVALQKNRYWRTQSGLSLDAGPFVA
                     ALEYASGKRATVVGKPEASFFRIALEDLGLAANEVAMVGDDVEVDVAGARRAGLSGIQ
                     VRTGKWRPGTEVEEADLVIDSIADLPEALELGGGER"
     gene            4402..4800
                     /locus_tag="VHM69_07470"
     CDS             4402..4800
                     /locus_tag="VHM69_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX2740271.1"
                     /translation="MKIVDVPVRTSGRYELVSITDEVKQAVREAGVDEGICIISSTHT
                     TAAVTVNEDYDRDVPRDLAAACRAFLDVLDVRFEHAEGNSDSHFLTSLFGQSQTLLVR
                     GGELDLGRWQGVFLAEFDGPRSRTVSVAVS"
     gene            4915..6306
                     /locus_tag="VHM69_07475"
     CDS             4915..6306
                     /locus_tag="VHM69_07475"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2740272.1"
                     /translation="MNGLVKGGLALGGVVLGLGALRRALNPTPRYASWEKPPYSEFEK
                     KVLVVGGGFGGYTVATDLCKMTQGRDDVGVLVIARDNYFTFWPMVPGIVSSDIDARNV
                     AQPLRRALILAGASFRRATIEGIDAERKVARAEGGIEFPYDQLIISLGGMPNFFGIPG
                     VEEHSMTMRGVEDAERVRNRVIERFEEVSLIREEVPDSKLTFVVIGGGATGVEVASEI
                     HSLVHENLAPDYPNIDPYRVRIYLIEAMPNILPELDPVLRRAARTRLVNERIEVLTNA
                     MAEEITGDLVKLKGGRQIATENVIWTAGNRPNQTIQDLGLPYDERTGIKVDLTLRAEG
                     HRDIWAVGDCAAVQDVRREDGAIVPPTAQAAIQEGHVAARNVLKTLDGEGELEEFKYK
                     PIGQLVELGSDFAVNEVMGVRFSGVIAAIFWRLAYLVRLNSPQSKVRVAADWIFGLLL
                     RPAVTQIRGSSNK"
     gene            6370..6939
                     /gene="pgsA"
                     /locus_tag="VHM69_07480"
     CDS             6370..6939
                     /gene="pgsA"
                     /locus_tag="VHM69_07480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX2740273.1"
                     /translation="MSLANQLTLIRLVAVVPVMVALYLPFEWARPLAVVLYAAAILTD
                     YFDGKIARRNNEVTGFGKLMDSIADKALIVSLFFALVGEGSMAVWMAAVMVIREFAVT
                     GLRMVALEAGEVIAANKWGKAKMNAQSIAVFILLLGQPGAQGWQGIVSQIGWWAMLVA
                     VVLTVLSGWSYLKDTPRILSITSKRDQRL"
     gene            6961..7806
                     /locus_tag="VHM69_07485"
     CDS             6961..7806
                     /locus_tag="VHM69_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740274.1"
                     /translation="MADGTEKRLRPAEVEMILRRASELNSRRWYRNSKETPSVSPEVL
                     VQVAAAAGIPEQDVRRAMWDMASEKTAEPHSFARKFYGQARLRAVREVERPAERTREH
                     LEGLLRLEQGLKLRRKTEVGSLWDSGDELGVVRRTLDFSGHRTLLKARIIELRVEGVE
                     EERCEANLTADLSNQRGEYLSLAGILGATLAVLFILAGMQSPLFFLGVLPGLAAPALG
                     FKFAYSRASLEIRRALDDILDAVEEVPPHEEEPQDRGDRPPGQIQGLKPIPRFAPDRS
                     KKDRP"
     gene            7857..>8827
                     /locus_tag="VHM69_07490"
     CDS             7857..>8827
                     /locus_tag="VHM69_07490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX2740275.1"
                     /translation="MPLSVLKEIFGFDSFRPGQEQAIRAVLDGRDTLAVMPTGGGKSL
                     CYQVPALMQESLTVIVSPLISLMKDQVDSLLQSSVTDAATLHSGLSPEERWEVERRVR
                     TGEVKMLYVAPERLRSLEFVLALRRAGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVR
                     DLGSPPVLALTATATPRVRQDILRSLKMRSPEVVVTSFNRPNLTYRVVPAKDKKHKLP
                     RILDIIRGYPPPGIVYATTRKECEELAASLRRSGVDAAPYHAGMGSADRAYVQERFMT
                     DEVAVVVATIAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGRDG"
BASE COUNT         1630 a         2743 c         2864 g         1590 t
ORIGIN      
        1 ccgccgccga ggtaaccaag agcccaggcc cgagaggaga cgcggtcgcg ctcgtcgggc
       61 tcagcgatct cgggcagaaa cgcgttgtag acgacgatcg acgcgccgaa cgacaggttc
      121 gcgattacaa agagcaggcc gccgaacagg taagctgagc cctggatcag gtaaagtccc
      181 atcgtggaga ttgcgccgat gtaggcgagc agcgccagga gtcgcttctt gcgctgtgtc
      241 cggtcggcga tggcgccggt gacaggcaac aagaagacct gcaagacgac ggagagcgag
      301 agcacgtaag ggaagaacga cccggcgaga attgggattc cgagcgggtg cacgtatccc
      361 cccgtgtccg ccgcgttctc ggttacggcg atgagatagg gacccagaaa gaccgtgaga
      421 accgtcgtag agaacgcgga gttggcccag tcgtagaagt accagccgcg ctgctcgcgg
      481 ttgcttgtcc gtttatctgc ctcaggcgtg gcactcgtgg tcgacacaat ccctccccac
      541 gctggtcgat ctctccgtcc gccggtgcgg cccggcgagg aggccactct gatctcttgc
      601 taactttttc atacgtaacc ttctcgatac cggcatgatt acacgagatg aatcatgagg
      661 cgtcccgcct cgagtcactg gtgttcgcca gtttcgccac tcgctcgcgc acggcctgga
      721 cgacctcctg ttgcgacgag gcagtctcaa gatcaccggc aaccagcggt tcgccgaagg
      781 tgacggtaac ctgctccaga cggcgcaaga actttcccct gggcatcgcc tcgtaggtgc
      841 cactgatgaa cacaggcacc acaggcactg aatagtagtt gagcaatatg cccatgcccg
      901 gcttgaactg ctgcagtcgt cccgtcggtg aacgctcgcc ttcggcgaac cagaccaggt
      961 tccttccgcg cttgaggacc gcggcgccga aagcaaggct cgagaggccg gcccgatcgg
     1021 ggtctaccgg taccacctgc gcgagacggc tgacgagacg ggttaacggg ttgccgaaag
     1081 ccgctcccgt ccatcctgcc cagtacgtgc ggcgcagaac gcggtcgtcg agcgctgcgg
     1141 ccaccgcgaa cgaatccagg tagctgacgt ggttcggtgc ggcgatgaaa gggccttcat
     1201 caggcaggtt ctccagaccc tccacccgca gacggaaggg cccgcgcatg agcatccggt
     1261 tgagggtgaa catgcctccc gccagcgccg aggccgctgg ccccagcggc gcgagccacc
     1321 gcttctggcg gtcgttgagg acctcctcgg gccgtgcgag aggcgaggtg tggacttcgg
     1381 tcccgccggc ttcggcctcc ctggctacct cgcgcagcag gtcgcggacg gtgtcgatgt
     1441 gctgtatcgc ttcttcgtca agatccacgc cggccttctc agcgatctcc agcgtcaggt
     1501 tgatccatcc cagcgagtcg atatcgaggt cgagctgtgg gctcgactca ggggtgaggc
     1561 cccggtcggg gtagcgattg gcaagcagat cccagacggt tgccgccgcc gggttaccca
     1621 tcagaacgcg gtccgcctcg gacatctctt ccaccgcgat gggtcctgtg gccgcctcgt
     1681 cctggctttc cttacccctt ttcgcacgct cgtagcgctc gtccaggacg tgcctgcgga
     1741 gcttgcccag gcgggtgtac tccagcggtt cgcggcttac cgcgtagtcg gagatccgct
     1801 ggtaggaggg caggcgtttc gacccctcct cgaccgcctc gcggatggac tgctggatgt
     1861 cgccgtctcc aagacgccgg atctgaacta tatcgggcac gatgagcgcg acgagacgac
     1921 cgtctttttg cagcacgccg acctcacgga tgtacgggtt ctcctggtag gctccttcga
     1981 cgtcctcagg ctggatgttc ttcccgctcg cggtcacaat cagggtggag gcccggcccg
     2041 tgacgtacag gtaaccgtca tcgtcgaagt agccgaggtc gccggtgcgg aaccatccat
     2101 cgtccgtgaa gaccttgctg gtctcttcag gcctgttgcg gtagccggag aagacgttcg
     2161 gcccgcgcgc gaggatctca ccttcccggc gaagcttgcc gtccaatcgt ctctgagatt
     2221 catcgggcac ggcagaggga tctacgctta tctccacgcc aggaaccggg cgtccgacac
     2281 tcccgagctt ttctccgtcg ggcgggttga gggtcagaag cggggcggtt tcggtgagac
     2341 cgtaaccgac ggcgactcgc catcccatgc cctccagctt ccatgccagg tcgggatcca
     2401 gggccgcccc gcctgaggcg agtacccgta gccttggccc gaaccgctcg cgcaatgggc
     2461 gcaagggaac cttgccgacg tcaaaccccg tcctgcggcg cagcctgaca cagaactgga
     2521 tgcctgctcc gaatagcgcg gccgccacac gtccactgga tttgattcgc tcttctatac
     2581 ccgaatacaa cgcgccgtag agcctgggga caccgatgat caaagtgact tccccttcct
     2641 gcaaagcgcg tacgagctgc ggtcccgtaa gggattgggg catgacgatc ggcacttccg
     2701 cggcgagcgg agtgagcatt cccatcacga aagggtagac gtggtggagc ggcaagggca
     2761 acaggatact gtcgtcctca gatacgagat ccgccccgag aagcgcatcg atctggaaga
     2821 ccaggttcct gtggctcagg ggtactccct tggcagtgcc tgtggtgccg gaggtgtaga
     2881 acagcgccgc agggtcgcca ggttcgaccc gaggcaactc cacttcgtcg tccgcgagca
     2941 ggagccgcca actgcgctcg tcgccttctc cggcgtccaa caagatagac cttggcccgt
     3001 catcaagatc gaggcgctcc acacgctcgg ccctgtcggc agtggtaaag atgaacctcg
     3061 tcccgctgtc atcgatgatg cgaccgagcg ccgcatcatc gagttgcgca tccaggggca
     3121 cgacaatggc cccggcgtgt atcacagcca ggcatgccac gacccactca ggacgattgc
     3181 cggcaaatat cgccacgtgg tcgccgcgtt cgacgccggc ccaccgcagg ccacgcccca
     3241 agcgccgcac gttgtcggag agctccaaat aggaccagcg ctccacgcct tcctcgcgta
     3301 gcgccactac ggccatccgc ccaccgcgcc aggagagcgt atcgaccagc catcgcagcg
     3361 tgcgctcctc gctcatgacc agccccgtac cctcgtttcg atcatccatg agatctctcc
     3421 tttacaccgt aaacttacaa cgtaagtata gaagccattg ttttcggttg tcaagggttt
     3481 cggatggttt ctttcgagga ttttgcgagg tgtttggggc aaagcttgga ggttgggagt
     3541 ggcgaggagc gcatccgggg ctttccgtgt ggcggtggag tagatgggtg ggcgctcttg
     3601 gggcgtggct tcaagtatgg cgcggcacta tgattaacgc atgagcgtca ggggtgtggt
     3661 attggatctc gacggaacgc tctacgttgg gggagagcct gtgcctggtg cgagggatgc
     3721 cgtcgagcgt ctaaaggctt ccggtctcag gctcaggtac gtcaccaata cgaccaggat
     3781 gccgcgtcgc gcggtggtcc ggagcctgag gacgctcggg ttccaggttg ctgagggtga
     3841 ggtcttcacg ccggcgtggg cggctgcgcg gttgatcggg gacggggcct gcctgccgct
     3901 cgtggacgac gctcttcgcg aggacctgga cggagtgagg ttcaccgaag attcacctga
     3961 gttcgtactc gttggcgatc tgggagaagg ttttacctac gcccgcctcg acaaggcatt
     4021 caggcgcctc atggacggtg ccgagctggt ggcgttgcag aaaaaccgtt actggcgcac
     4081 gcagagcggg ctctccctgg atgcagggcc gttcgtggcg gcactggagt atgccagcgg
     4141 gaagcgggcg accgtcgtag ggaagccaga ggcgagcttc ttccggatcg cgctcgaaga
     4201 tctcgggctc gcggccaacg aagtcgccat ggtgggtgac gacgtcgaag tcgacgttgc
     4261 aggagccagg agagcagggc tctcaggcat acaggtgaga actggaaaat ggcggcccgg
     4321 taccgaagtg gaggaggctg acctcgtaat cgacagcata gcggatctgc cggaggctct
     4381 ggagcttggg ggaggagaga gatgaagatc gtggacgttc ctgtaaggac gagcgggcgt
     4441 tacgagctcg tttcgatcac ggacgaggtc aagcaggcag tcagggaggc cggcgtggac
     4501 gagggtatct gcattatctc ctcgacgcac actaccgcgg ctgtgactgt aaacgaggac
     4561 tacgatagag acgtgccgcg tgacctggcc gccgcctgcc gcgccttcct cgacgttctt
     4621 gacgtgcgct tcgagcacgc cgagggtaac agcgactctc acttcctgac gagtcttttc
     4681 ggacagtcgc agaccctgct tgtgaggggt ggggagcttg acctcggacg ctggcagggc
     4741 gtcttcctcg ccgagttcga cggaccgcgg agcaggacag tcagcgtggc tgtctcgtag
     4801 tcggcttccc aggtctgccc tgtttagtta tatccaaaca ttcggtaaga tgtgatacag
     4861 ggcgcgggct cggatgtcgt gaccggcctg aattagtctg tgtgggaggc ttgcatgaac
     4921 ggattggtca agggcgggct ggcgctcggc ggtgtggtct tggggctcgg ggctctgagg
     4981 cgggcgctca acccgacgcc caggtacgct tcctgggaga agcctcccta cagtgagttc
     5041 gagaagaagg tgcttgtcgt tggcggaggt ttcgggggat atacggtcgc aaccgacctg
     5101 tgcaagatga cacagggccg cgacgacgtt ggggtcctgg tcatagcgcg cgataactac
     5161 ttcactttct ggccgatggt gccaggcatc gtcagcagcg acatagacgc ccgaaacgtc
     5221 gcccagccct tgaggcgagc cctcatcctc gcaggcgcca gcttcaggcg ggccacgatc
     5281 gagggcatcg acgccgagcg gaaggtcgcc agggccgaag gcggtatcga gttcccttac
     5341 gaccagctca tcatctccct cggcgggatg cccaacttct tcggcatccc cggcgtcgag
     5401 gagcacagca tgaccatgag aggcgtcgaa gacgccgagc gcgtccgcaa ccgggtcatc
     5461 gagcgcttcg aggaggtctc cctcatcagg gaagaggtgc ctgattccaa gctgaccttc
     5521 gttgtcatag gaggcggggc tacgggggtc gaggtggcct ccgagatcca cagcctcgtc
     5581 cacgagaacc tcgcaccgga ttatccgaac atcgacccgt acagggtaag gatctacctc
     5641 atagaagcca tgccgaacat actgcccgag ctcgaccccg tgctgaggcg cgccgctcgc
     5701 accaggctcg tcaacgaacg catagaggtg cttaccaacg cgatggcgga ggagatcacc
     5761 ggtgacctcg tcaaactcaa gggcggcagg caaatcgcca cggagaacgt gatctggacg
     5821 gctggcaacc gtccgaacca gacgatacag gatctcggcc ttccctacga cgagcggacc
     5881 ggcatcaagg ttgacctgac gctgcgggcg gaaggccatc gtgacatctg ggccgtcgga
     5941 gactgcgcag ccgttcagga cgtgagacgt gaggacggcg caatcgtccc gcccaccgcc
     6001 caggccgcga tccaggaggg ccacgtggcc gcccgcaacg tgctcaagac cctggacggc
     6061 gagggcgaac tcgaagagtt caagtacaag cccataggcc agctcgtcga gctcgggagc
     6121 gacttcgccg tcaacgaggt gatgggcgtc aggttcagcg gggtgatcgc ggccatcttc
     6181 tggcggctcg cctacctggt gcgcctgaac agcccgcaaa gcaaggtgcg ggtcgccgcg
     6241 gactggatct tcggcctcct cttgcgaccc gccgttaccc agatccgggg ctcctccaac
     6301 aaatagtctt gagacgcggc tacaatggtg ctacgttggc catgaccgaa agaagagaga
     6361 agagtacgcg tgagcctcgc taaccagctg accctgatcc ggctcgtcgc cgtcgtgccc
     6421 gtaatggtcg cgctatacct gccgttcgaa tgggcgcgtc ccctcgccgt ggttctgtat
     6481 gcggcagcga tcctgaccga ctacttcgac ggaaaaatcg cccggcgcaa caacgaggtc
     6541 acgggcttcg gcaagctgat ggactccatc gccgacaagg ccctcatcgt ctcgctgttt
     6601 ttcgcgctcg tcggggaggg ttcgatggcc gtgtggatgg ctgcggtcat ggtcatccgg
     6661 gagttcgccg tgacgggact caggatggtc gccctcgagg cgggggaggt catcgccgcg
     6721 aacaagtggg gcaaggcgaa gatgaacgcc cagagcatcg ccgttttcat actcctgttg
     6781 ggccaaccag gcgcccaggg ctggcaggga atcgtgtccc agatcggatg gtgggcgatg
     6841 ctcgtcgccg ttgtcctcac cgtactctcg ggctggtctt atctgaagga cacgccgagg
     6901 atcctttcga tcacctcgaa gcgggatcaa aggctctaaa agctgtagaa tcacgtccca
     6961 atggcggacg gtacagaaaa acgcctgaga ccggcggagg tcgagatgat cctgcgccgc
     7021 gcctcagaac tgaactctcg tcggtggtac cgcaactcca aggagacacc ttcggtctct
     7081 cccgaggtcc tcgtgcaggt ggccgcggcg gcgggtatcc ccgaacagga cgtgcgcagg
     7141 gcgatgtggg acatggcctc tgagaaaacg gccgaacccc actcgttcgc ccgcaagttc
     7201 tacggccagg cacgtctgcg ggccgtgcgc gaggtcgagc gcccggccga gcggaccagg
     7261 gagcacctgg aaggactgtt gcgcctcgaa cagggtctca agctcaggcg caagacggag
     7321 gtcggctccc tctgggattc tggggacgag ctcggggtcg ttcgcaggac gctcgacttt
     7381 tcggggcacc gcacgctgct gaaggccagg atcatcgaac tgcgcgtcga aggcgtcgaa
     7441 gaggagcgat gcgaagcgaa cctgacggcc gacctctcca accagcgtgg ggagtacctc
     7501 tcgctcgccg gcatcctcgg ggctacactg gccgtcctat ttattctcgc cgggatgcag
     7561 agcccgctct tcttcctggg cgttcttcca gggctcgccg ctccggccct cgggttcaag
     7621 ttcgcctaca gcagggcgtc tctggagata aggcgcgccc tcgacgacat cctggacgcc
     7681 gtcgaagagg tcccgcccca cgaagaggag ccccaggatc gcggcgaccg tcccccaggc
     7741 cagatacagg gcctcaaacc tatccccagg ttcgcccccg atagaagcaa gaaggataga
     7801 ccctagtcct cacctgcact ttgtgtatgt ggggatgagg ccgtatattt agcttcgtgc
     7861 ctctaagcgt cctcaaggag atcttcggct tcgactcgtt caggcccggc caggagcagg
     7921 cgatccgggc cgttctcgat ggcagggaca ccctggccgt gatgccgacg gggggaggca
     7981 agagcctctg ctaccaggtg cccgcgctga tgcaggagag cctgaccgtg atcgtatcgc
     8041 ccctgatctc actgatgaag gaccaggtgg actccttgct tcaaagctcg gtcacggatg
     8101 ccgcgaccct gcactccggg ctctcgcctg aggagcgctg ggaggtcgag cggagggtgc
     8161 ggacgggaga ggtgaagatg ctctacgtcg cccccgagcg gctcaggtct ctcgagttcg
     8221 tcctcgcgct gcggcgcgcg ggtgtcggcc tcttcgtcgt tgacgaggcc cactgcatct
     8281 cggagtgggg ccacaacttc cgccccgact acctgttctt gccgcgcgcg gtgagggacc
     8341 tcggtagccc gcccgtcctc gccctgaccg cgacggcgac cccccgcgtc cgccaggaca
     8401 tccttcgctc tctgaagatg cgctcgcccg aagtggtggt gactagcttc aatcgcccga
     8461 acctgacata cagggtcgtg cctgcgaagg ataagaagca caagctcccc cgcatactcg
     8521 acatcatccg tggctacccg ccgcctggga tcgtctacgc gacgacgcgc aaggaatgcg
     8581 aggaactcgc cgccagcctg cgccgatccg gcgtcgacgc cgccccttac cacgccggca
     8641 tgggctccgc cgacagggcc tacgtccagg agcgcttcat gaccgacgag gtcgccgtcg
     8701 tcgtcgcgac catcgccttc gggatgggcg tggacaagcc gaacgtgcgc ttcgttatcc
     8761 attcgagcgt cccgggaagc ctccccgcct acatacagga gtcgggcagg gcgggaaggg
     8821 acgggga
//
LOCUS       DATAIR010000306         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_296984, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000306 DATAIR010000000
VERSION     DATAIR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296984"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VHM69_07495"
     CDS             complement(<1..612)
                     /locus_tag="VHM69_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2740276.1"
                     /translation="MEKVDLYKVLEVSKEASQDEIRRSYRRLARKYHPDANPGDRQAE
                     ERFKEIQHAYEVLSKPEKRREYDEGPRTFFGSQNAGQRRSGTADFEDLSDLLGGFGNL
                     GDIFGRTSSTTQRAPAKGENITVNVRLNFKDALNGVTTRVGVPVEEACDECRGTGAAP
                     GTAPRTCPECGGRGVQSRDQGFFALSTPCSRCGGEGRIIEKPCA"
     gene            complement(616..1260)
                     /locus_tag="VHM69_07500"
     CDS             complement(616..1260)
                     /locus_tag="VHM69_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX2740277.1"
                     /translation="MSSEENKRDPELRGDDVEPEEEAVEESKDSQAPVENTAHDSDAP
                     PDTEGEPAPVPDEESEPEQDELAVILQELEAARQERDQYLDALRRLKAEFENSRKRQE
                     RERTRILSLASERLVQELLPVLDNLDRALEAGGDIREGVQATREQLADVLGNEGLLPV
                     ASDGQPFDPNVHEAVMGQPSEEHEEGTIIQTFQRGYLLNGKPIRPAKVVVAKQV"
     gene            complement(1337..>2181)
                     /locus_tag="VHM69_07505"
     CDS             complement(1337..>2181)
                     /locus_tag="VHM69_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX2740278.1"
                     /translation="HKGINPDEVVAVGAAIQAGVLAGEVKDVLLLDVTPLSLGIETKG
                     GVFTKLIERNTTIPTRKSEVFTTADDNQGSVEIKVYQGEREIAAYNKIIGNFQLVGIP
                     PAPRGVPQIEVAFDIDANGIVNVGAKDLGTGKEQRITITASSGLSDAEIQQMMQDAES
                     HAEEDQRRREEADVRNNADNLVYSIERSLKDVDGKVDPNTKLEIEQAIKETKEALAGG
                     DVEEIKRKQEALLTASHKLSEVLYSQAQQQQDASSAGATDNDDLVEDAEVVDDEDEDG
                     EERK"
BASE COUNT          306 a          729 c          646 g          500 t
ORIGIN      
        1 cgcgcacggc ttctcgatga tcctgccctc gcccccacac ctcgaacaag gcgtcgagag
       61 cgcgaaaaac ccctgatccc ggctctgcac ccccctgcca ccacactcgg gacacgtcct
      121 cggcgccgtc ccaggtgccg cccccgtgcc cctgcactcg tcacacgcct cctcgacagg
      181 gaccccgacc cgcgtcgtga cgccgttcaa cgcatctttg aaattcaatc tgacgtttac
      241 ggttatgttt tcgccttttg ccggcgccct ctgggtggta ctggacgtac gtccgaagat
      301 atcgcccagg tttccgaagc cgcccagtag atcagagagg tcctcgaagt ccgcggtgcc
      361 agaccttcgc tgtccggcat tctgcgagcc gaagaaggta cgggggcctt catcgtattc
      421 ccgtcttttc tcgggtttcg agaggacttc gtaggcgtgc tgaatctcct tgaagcgctc
      481 ctcggcctgc ctgtcaccag ggttggcgtc ggggtggtac ttgcgcgcca ggcggcggta
      541 ggagcgccgg atctcatcct gcgaggcctc tttggatacc tcgagaactt tgtagagatc
      601 caccttctcc ataccctaaa cctgcttcgc aactacaact ttcgccgggc gtatcggctt
      661 gccgttgagc aggtatcccc tctggaaggt ctggatgatg gttccctcct cgtgctcttc
      721 ggagggctgt cccatgacgg cctcgtggac gttcgggtcg aatggctggc cgtccgaggc
      781 gacggggagc agtccctcgt tgcccagtac gtcggcgagc tgctcgcggg tagcctgcac
      841 gccctcgcgg atatctccgc ctgcttcgag ggcgcggtcc aggttgtcca gtactgggag
      901 tagctcctgc acgagcctct cggaggccag ggagagtatg cgggtgcgct cccgctcctg
      961 gcgcttgcgc gagttctcga actcggcctt caggcggcgc agggcgtcca ggtactggtc
     1021 ccgctcctgg cgcgcagcct cgagctcttg caaaatcacc gctagctcgt cctgctccgg
     1081 ttcagactcc tcgtcgggga cgggggccgg ctcgccttcc gtgtctggtg gcgcgtccga
     1141 atcatgtgcc gtgttctcga cgggggcctg cgagtccttg gactcctcta ccgcctcttc
     1201 ttccggctcg acatcgtcgc cccggagttc ggggtcgcgc ttgttctcct cgctgctcaa
     1261 agctccctag tcctcctgtg gctgccgtgc gggggcagcg gagcgagtct cctcactcgc
     1321 gcggccgccc ggcctcttat ttgcgctcct cgccgtcctc gtcctcatcg tcgacgacct
     1381 cggcgtcttc cacaaggtcg tcgttatccg tggcgcctgc ggagctggca tcttgctgct
     1441 gctgggcctg gctgtagagg acctccgaga gcttgtggga ggctgtcagc agcgcctctt
     1501 gcttgcgctt gatctcctcg acgtcgccgc cggcgagcgc ctccttggtc tcctttatgg
     1561 cctgttctat ctccagcttg gtgttcgggt ccaccttgcc gtcaacgtcc ttcaaagaac
     1621 gctcgatgga gtacaccagg ttgtccgcgt tgttgcggac atcggcctcc tcgcggcggc
     1681 gctggtcctc ctcggcatga gactccgcgt cctgcatcat ctgctggatc tccgcgtcag
     1741 agaggccgct cgaggcagta atcgtgatcc tctgctcctt gcccgtcccg aggtccttcg
     1801 ccccgacgtt gacgatgccg ttcgcgtcta tgtcgaacgc aacctcgatc tgaggtacac
     1861 ccctgggcgc cggcggaata ccgacgagct ggaagttgcc gatgatcttg ttgtacgcag
     1921 ctatctcgcg ctcgccctgg tagaccttga tctcgaccga accctgattg tcgtcagcgg
     1981 tggtgaatac ctcactctta cgtgtaggta tagtggtgtt tcgctcgatg agcttggtga
     2041 acaccccacc cttggtctct ataccgaggg agagtggcgt gacatcgagg agcagcacgt
     2101 ctttgacctc tcctgcgagg acaccggcct ggatggctgc tccgacggcg acgacctcgt
     2161 cagggttaat acccttgtga g
//
LOCUS       DATAIR010000307         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_297074, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000307 DATAIR010000000
VERSION     DATAIR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297074"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..585)
                     /locus_tag="VHM69_07510"
     CDS             complement(193..585)
                     /locus_tag="VHM69_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740279.1"
                     /translation="MAAIIYAAVSAGVVAFQVALAAGAPWGSYAMGGAFPGQFPPTLR
                     IAVLVPAALIVGMAAVVMSRAGLVLAGWSHASRWLVWVVVAFAAVSLVLNLITPSAGE
                     RTIWAPVALLLLVSSAVVAIERPSGRQS"
     gene            complement(684..1385)
                     /locus_tag="VHM69_07515"
     CDS             complement(684..1385)
                     /locus_tag="VHM69_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX2740280.1"
                     /translation="MNTSHADTLDTRWNWLYKVGGAAALFGVAIIPAQLAIFIAWGQP
                     GTALGWFTLFQENKLAGLLAFEFLFVVNAALGISTTLALYIALRQVNESLMTLALALG
                     LVEAIALIMARPAFEMLSLSNQYAGATTDAQRAMFLAAGEAMLATFDGTAFHVSYNIF
                     SVFLLMVSLVMLRSNIFGRVTASMGILAAILNWGLYVPDIGIFLSILSIVPLAIWNVL
                     IARRLFQLGSAIRKG"
     gene            complement(1396..2388)
                     /locus_tag="VHM69_07520"
     CDS             complement(1396..2388)
                     /locus_tag="VHM69_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740281.1"
                     /translation="MRPARSKVARLPVKRGLTLPRRLSLVVAVLTAAASTAGLVLGPA
                     GLYGTDPKRALGVTEGEAGLLLPGFLGQDVFNLVVALPLLLGTMWLAQRGSLAGLLLW
                     PGALFYVLYWYVLYLIGAPFSVLFLLYVPLVTLSACAIIALVSSIDDEEVRRRLAGAV
                     PARIIGGILVALALLTIAQDGSGAFVTALGENAPIDPVARHVWIADLALQAPAVLAGG
                     VLLWRREALGYVAGAGLLLQYGLSVVGFVASMALRAVLTASSLDMVALVILLVFGVIC
                     FVPLASFVRAAMIGDANSRSAVVVGASNEPVEPVVKGAEAADLAVQSQPHRELE"
     gene            complement(2490..>2840)
                     /locus_tag="VHM69_07525"
     CDS             complement(2490..>2840)
                     /locus_tag="VHM69_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740282.1"
                     /translation="GSVPQSVTTNGTPTNGVHVLDMAWMLPAMGLTAIWLWRGLALGY
                     ALAGALLTFMPLLLLSIMGMVVSMNLHGQPVAPGMAVVFGVVAATSLGLMVWYLKGLS
                     GDPSTSTNHSAGQL"
BASE COUNT          630 a          902 c          867 g          441 t
ORIGIN      
        1 tccctcctcc ctagcaatct ctctgtgctc cgatgggtcc gggctcgaat ccgtccacgt
       61 tcgtgataac actctcttca accgaagtcc ttccccataa tggctcggaa ggtggccatc
      121 ttcgcgggcg gtaaagcgaa agcatccaaa cacgccacat cgccctgggc cttaccacat
      181 catcgcctcc attcagctct ggcgtcccga gggccgttcg atggctacca ccgcgctgct
      241 caccagcagc agcaacgcca ccggtgccca gatggtgcgc tcaccggcgc tgggcgtgat
      301 caggttgagc accagactca cggccgcaaa cgcaacgacg acccacacga gccagcgcga
      361 cgcgtgagac catccggcaa ggacgagccc ggcccgcgac atcacgaccg cggccatccc
      421 gacgatgagc gcggctggga caagcacggc gattcgcagt gtcggcggaa actgtccagg
      481 gaacgcgccg cccatcgcgt acgagcccca cggagcgcca gcggccaacg ccacctgaaa
      541 ggcgacgacg cccgcggaaa ctgccgcata gatgatcgct gccacacgtc tgacgctcat
      601 gcggtcaggg cgatggcgta gagtgaatag gagatcaagg gatagtttga aacaatgttc
      661 ccacccgtca gcgacatcac agactacccc tttcgaatcg cgctgcccag ttggaagagc
      721 cttcgggcga taaggacgtt ccagatcgcc agagggacaa tggagagtat cgagaggaag
      781 atacctatgt ccggcacgta aagcccccag ttgagtatgg ccgcaagaat tcccatagag
      841 gcggtcactc ggccaaagat gttgctccgc aacatgacga gcgagaccat cagaagaaat
      901 acgctgaaga tattgtaact aacgtgaaag gcagtgccgt cgaaggtagc cagcatcgct
      961 tcccctgccg cgaggaacat cgctctctgc gcatccgtcg tcgcgcccgc gtactggttg
     1021 ctgagggaga gcatctcgaa ggccggcctc gccataatga gagcgatggc ttccaccagg
     1081 ccgagggcaa gggctaaagt catgagggat tcgttgacct gtctgagggc gatgtagagg
     1141 gcgagtgtag tcgagatccc gagagcggcg ttgacgacga aaaggaactc gaaggccagg
     1201 agccccgcga gcttattttc ctgaaacagg gtgaaccagc cgagtgctgt gccgggctgc
     1261 ccccacgcga tgaagattgc gagctgggcc gggatgatgg ccaccccaaa cagggccgcc
     1321 gcacccccga ctttgtagag ccagttccag cgcgtatcca gggtatctgc atgagatgta
     1381 ttcatcgcga tgtactcatt ccaactcccg atgtggctga ctctgcacgg caagatcagc
     1441 tgcctccgcc cctttcacaa ccggctccac gggttcattc gatgcaccta ccacaaccgc
     1501 acttctacta tttgcatcgc cgatcatcgc agctcgcacg aaagacgcga gtggcacaaa
     1561 gcagatcacc ccgaagacta ggagtatgac aagggcgacc atgtcgagcg acgaggcggt
     1621 cagcacagct cgaagcgcca tgctggccac gaaccctacg actgagaggc cgtactggag
     1681 cagcaacccc gcgccggcca cgtaccctag agcttcccgc cgccacaaca gcacgcctcc
     1741 tgcgagcacc gcaggagcct gcagcgcgag gtcggcgatc cacacgtggc gcgccacagg
     1801 gtcgatcggc gcgttctcgc caagagcggt cacgaaggcg ccgcttccat cctgggctat
     1861 cgtcaggagc gccagggcta ccaggatgcc gccgatgatc cgcgcgggca cggcacccgc
     1921 gagccgccgc cttacttcct catcgtcgat gctagagacg agggcgatga tcgcgcaagc
     1981 actcagcgta acgaggggga catatagcaa gaacagcacg ctgaacggcg cgccgatgag
     2041 gtagagcacg taccagtaca gaacgtagaa gagcgcccca ggccacagca gcagcccggc
     2101 gagcgagcca cgctgggcga gccacatagt accgagcagg aggggcaaag ccacgaccag
     2161 gttgaacacg tcctggccaa ggaacccggg gaggagtaga cctgcctcgc cttctgtcac
     2221 gccgagggcg cgcttcgggt cggtgccgta gagtccggcg gggccgagca cgaggccggc
     2281 ggtggaggca gcggccgtga ggacggcgac cacgagcgag aggcgacggg ggagcgtcag
     2341 tccacgtttg acaggtagcc tggcgacctt ggatctggct ggcctcatag agtacctcct
     2401 tgcctccggc ggatcggtgg tccgctacta aggcgaagcg tatcggcatg cagctcttgc
     2461 ttcctgagca agatggctca tttgtatggt cagagttgac cggccgaatg gttcgtactg
     2521 gtagaaggat cgcccgaaag ccctttcagg taccagacca tcaggcccag gctggtcgcc
     2581 gccacgacgc cgaagactac ggccatccca ggcgccacgg gctgaccgtg gaggttcata
     2641 gagaccacca tccccatgat ggagagcagg agcaagggca tgaaggtgag cagggcaccg
     2701 gccaacgcgt agcccagggc caacccgcgc cacagccaga tcgccgtcaa tcccatcgca
     2761 ggtagcatcc acgccatgtc aagcacgtga acgccgttgg tcggggttcc gtttgtggtc
     2821 acgctctgcg gcacacttcc
//
LOCUS       DATAIR010000308         5103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_434821, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000308 DATAIR010000000
VERSION     DATAIR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434821"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VHM69_07530"
     CDS             complement(<1..819)
                     /locus_tag="VHM69_07530"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740283.1"
                     /translation="MGEGKGRGWSLGLTGLLTPVTLWLGLFFLVPLLLILAYSFGTSG
                     IYGGITLGFNPGNYLKVFDPLYLEIIGRTFFIAALNTLLCLALGYPLAYFIVFRARRW
                     RNALILLVMVPFWTSLLIRAYAWVVILGGNGVANRTLQFLGITDEPLTLIFTPEAVLM
                     GMVYSYLPFMVLPLYAALEKFDTRLKEAAQDLGASRWHTFWRVTFPLSMPGVIAGSIL
                     VFIPSAGEFVIPDLLGGSRTMMTGNLIQQQFLQARDWAFGSALSVLLAVLLLGAI"
     gene            complement(821..1927)
                     /locus_tag="VHM69_07535"
     CDS             complement(821..1927)
                     /locus_tag="VHM69_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2740284.1"
                     /translation="MGRGKRISRGDFLRAMGAASVAGSTLSILACQPNTTPQGGGGGG
                     GPEEKQLNFYNWADYVAKSTIPSFEKKTGIQVTQDYFSSNEDLLAKLQAGGTGYDVIV
                     PSDYMVAIMAKEEIIQKLDKSKIPNAKNVGPEYEGLSYDPNNEFSLPYQWGTTGILYN
                     KKEIGQLDESWDPMWDPEFDGKVAMLNDTRETLGAALYKLGYSVNATDQEQLDEAKAE
                     LIKQKPLLSGYFDSTESRPLVVNGDLLLGHVFSGDAFLALSSNDDLDYIIPKPAATRW
                     TDNMCIPNGAEHPDNAHKFINHILDAGVGAKLSNYTYYNTPNAAALPKISGALKNLPG
                     YDLPPEVFERLEVIEDTGEVTREYERIFTEVKSS"
     gene            complement(1930..3057)
                     /locus_tag="VHM69_07540"
     CDS             complement(1930..3057)
                     /locus_tag="VHM69_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2740285.1"
                     /translation="MEERRISERPPEDTGQDAKRVVAVELSGVTKRFGEFVAVDDLTL
                     DIYEGEFFSLLGPSGCGKTTTLRMIAGFEEPTEGGISVDGDPVRGVPPYRRPVNTVFQ
                     SYAIFPHLNVFDNVAFGLRRSGVKGEELQHRVTDACEMVQLSGFEKRKPNMLSGGQQQ
                     RVALARALVNRPKVLLLDEPLGALDLKLRKEMQLYLKNLQHEVGITFIYVTHDQEEAL
                     TMSDRIAVMNEGKVQQVADPPTLYELPKNRFVADFIGQTNVFSGTVESVSGEYVALRI
                     PAGTKVEAISREVGLEVGAQAHAAVRPEKVRFGSEGDNVSTASIRQIVYLGVSTQYIA
                     ELPGGEKLVLYQQNSREENTPDVGQEVPVAWDARNCLILGG"
     gene            complement(3213..4637)
                     /locus_tag="VHM69_07545"
     CDS             complement(3213..4637)
                     /locus_tag="VHM69_07545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2740286.1"
                     /translation="MTRAVSASRTARAGGKDYRSYSFWLETAGEELAPRSSLPGPMEA
                     DVAILGAGFTGLWTAYYLLKKEPSLRVVILEAEISGFGASGRNGAWCNSAFPVSPGDL
                     QRRFGRAATRGLLLEMRDAVEEVGKVAAAEEIDAQYFHGGQLRVARGEHQLPGIEEAY
                     ESLRALGLEEGLRLLDAEEMAGRVRITGARGALYNPHCATIHPARLARGLGRAVERLG
                     GEIFEQTPVTDYESGTNPRLMTDSGEVRARTVVLAGEAYLARLPKLRRQVLPIYSLIV
                     LTEPLSEEQWAEIGWEGRECVASNRYTVDYLSRTADGRILFGGRGAPYHYGSRIEDEY
                     DRHEPTHEKLRRTAREWFPAIEGARFTHAWGGPLAVPRDWMPTMSYDPASGVATARGY
                     TGQGVATANLSGRTLADLILGRQTAITRLPTVNHETRPWEPEPLRWLGTRYVQRGLMR
                     VDDRAEKTGEPPTGGTLAEKLGRH"
     gene            complement(4641..>5103)
                     /locus_tag="VHM69_07550"
     CDS             complement(4641..>5103)
                     /locus_tag="VHM69_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2740287.1"
                     /translation="RSKAPMFMHGSTFGGHPVSSAVAIENINIIEREGLLQNVHDLEG
                     HFGDELRRMAADHPIVKEVRGMGFFWAVEINPEREDGTPLEGDEYQKYFKGVVSRKLL
                     EGGLICRFDDKEDPVIQFSPALVADKEIISKIAGITDDALTELERELGYRS"
BASE COUNT          842 a         1636 c         1602 g         1023 t
ORIGIN      
        1 gatcgcgccc agcagcagga cggccaggag caccgagagg gcgctcccga aggcccagtc
       61 gcgggcctgg aggaactgct gctggatgag gttgcccgtc atcatggtgc gcgagccgcc
      121 gaggaggtca gggatcacga actcccccgc ggacgggata aaaacgagta tgctgccggc
      181 gatcacgcca ggcatgctca gggggaacgt gacccgccag aaggtgtgcc accggctcgc
      241 gcccaggtcc tgggcagcct ccttcaggcg ggtgtcgaac ttctccaggg cggcgtaaag
      301 cggcagtacc atgaacggca ggtaggaata caccatgccc ataaggaccg cctcaggggt
      361 gaagatcagg gtgaggggct cgtcggtaat acccaagaac tgcaacgtcc tgttcgctac
      421 gccgttgcca cccaggatca ccacccacgc gtaggcccgg atcaggaggc tcgtccagaa
      481 agggaccatg acgagcagga tcagggcgtt cctccatcgc cttgctctga agacgatgaa
      541 gtaggcaaga ggatacccga gcgccagaca cagcagggtg ttgagcgcgg ctatgaagaa
      601 ggtgcggccg atgatctcga ggtagagcgg gtcgaagact tttaggtagt tgcccgggtt
      661 gaagccgagc gttatgccgc cgtagatgcc gctcgtcccg aaggagtaag ccaggatgag
      721 caggagcggc acgaggaaga agagcccgag ccacagcgtg acgggggtga gcaaccccgt
      781 cagccccaga gaccagcctc gtcctttgcc ctctcccatg ctaggagctc tttacctcgg
      841 taaagatacg ctcgtattcg cgcgtcacct cacccgtgtc ctcgatgacc tcgagcctct
      901 cgaagacctc gggcggcaag tcgtaacccg gcaggttctt cagcgccccg gatatcttcg
      961 gcagcgcggc cgcgttgggc gtgttgtagt acgtgtagtt cgagagcttc gcgccgaccc
     1021 cggcgtccag gatgtggttt atgaacttgt gggcgttgtc ggggtgctcg gcgccgttgg
     1081 ggatgcacat gttgtccgtc cagcgggtcg ccgcgggctt cgggattatg tagtcgaggt
     1141 cgtcgttcga agagagcgcg aggaaggcgt cgccggagaa cacatggccg agcaagaggt
     1201 cgccgttcac gacgagcggc ctgctctccg tcgaatcgaa gtagccgctt agcaagggct
     1261 tctgcttgat cagctcggcc ttggcctcgt ccaattgctc ctggtccgtg gcgttgacgg
     1321 agtagccgag cttgtagagg gcggcaccca gggtctccct ggtgtcgttg agcatggcga
     1381 ccttgccatc gaactcgggg tcccacatag ggtcccagga ttcgtcgagc tgtccgatct
     1441 ctttcttgtt gtacaggatg cccgtggtgc cccactggta ggggaggctg aactcgttgt
     1501 tggggtcgta ggagagaccc tcgtactcgg ggccgacgtt cttggcgttc gggatcttgg
     1561 acttgtccag cttctggatg atttcctcct tggccatgat cgccaccatg tagtcgctag
     1621 gcacgatgac gtcgtagcct gtccctccgg cctgcagctt ggccagaagg tcctcgttgg
     1681 aggagaagta gtcctgcgtg acctgtatgc ccgtcttctt ctcgaagctc gggatggtgc
     1741 tcttcgcgac gtagtcggcc cagttgtaga aattgagttg cttctcttcg ggaccaccgc
     1801 cacctccgcc gccctgtggc gtcgtgttcg gctggcatgc caggatggag agcgtggagc
     1861 cggccacgct cgctgccccc atcgccctca ggaagtctcc acgcgagatc ctctttcctc
     1921 tacccatgtc taacctccta gtatcaagca gttacgggcg tcccacgcca cgggcacttc
     1981 ctgtcccacg tcgggggtgt tctcctcacg tgagttctgc tggtagagca ccagcttctc
     2041 accgccggga agctccgcga tgtactgggt actgaccccg aggtagacga tctgcctgat
     2101 gctggctgtg ctcacgttat cgccctcgct gccgaaccgg accttctcgg gtctcacggc
     2161 ggcgtgcgcc tgggccccta cttccaggcc gacctcacgg gagattgctt ccaccttcgt
     2221 ccccgcaggg atgcgcaacg caacgtactc gccgctcaca gactcgaccg tgccggagaa
     2281 gacgttcgtc tggccgatga agtcagcgac gaagcggttt ttcgggagtt cgtagagggt
     2341 ggggggatcc gcgacctgct gcaccttgcc ttcgttcatc accgcgatcc tgtcgctcat
     2401 cgtgagcgcc tcctcctgat cgtgggtgac gtagatgaag gtgatcccga cctcgtgctg
     2461 caaattcttg aggtagagtt gcatttcttt tcttagcttg aggtcgagtg cccctaaggg
     2521 ctcgtcaagg agcaacacct tcggcctgtt gacgagtgct cttgcgaggg cgaccctctg
     2581 ctgttggccg cccgagagca tgttcggctt gcgcttctcg aagccggaga gctgcaccat
     2641 ctcgcaggcg tccgtgaccc tgtgctgcaa ctcctccccc ttgacgcctg accgtctcag
     2701 gccgaaggcc acgttgtcga agacgttgag gtgggggaag atggcgtacg actggaagac
     2761 ggtattgacc ggcctcctgt atggagggac gccgcgtacc gggtctccat cgacggagat
     2821 gccgccctcc gtcggctctt cgaagccggc gatcatgcgc agcgtcgttg tcttgccgca
     2881 gccgctcggg ccgagcagcg agaagaactc gccctcgtag atgtcgagcg tgaggtcgtc
     2941 gaccgccacg aactccccga agcgcttggt aaccccggac aactccacgg cgacgacccg
     3001 tttcgcatcc tgcccggtgt cttcgggtgg acgctcactt atacgccgct cttccactat
     3061 cagcctcctg tagtgtccat aaaggcctgg agaaccgttc tcatccggag tccaccccgg
     3121 ccggcgaccg tgctgcgcgc gacgccgttc gtccccgtgt cctgcggttg tctgatgatt
     3181 tgtttctcat tgttcgtgcc ccaggattct actcagtgtc tgccgagctt ttctgcgagg
     3241 gtcccgcctg tcgggggctc gcctgttttc tccgcgcggt cgtccaccct catcaggccg
     3301 cgctgcacgt acctggtgcc caaccagcgc aagggttccg gctcccaggg acgcgtctca
     3361 tggttgacgg tgggcaggcg ggttatggcg gtctggcggc cgaggatcag gtcggccaga
     3421 gtccgccccg agaggttggc ggtagcgacc ccctgccccg tatatcctcg cgcggtagcg
     3481 accccgctgg ccgggtcgta ggacatggtc ggcatccaat cgcggggcac ggcgagcggc
     3541 cctccccacg cgtgggtgaa ccgcgcccct tcgatggccg ggaaccactc cctcgccgtg
     3601 cggcgcagct tctcgtgcgt gggctcgtgc ctgtcgtatt cgtcttctat gcgcgagccg
     3661 tagtggtatg gtgcgcccct gcccccgaaa agtatgcgcc cgtcggccgt acgtgagagg
     3721 taatctacgg tgtagcggtt ggacgcaacg cactcgcgtc cctcccatcc gatttcagcc
     3781 cactgctcct cggagagcgg ctcggtgaga acgattagag agtagatggg caacacctga
     3841 cggcgcagtt tgggtagacg cgccaggtac gcttcccccg cgagcacgac cgtgcgggcg
     3901 cgcacctcgc ccgagtccgt catcagcctc gggtttgtgc cgctttcgta gtccgtgacg
     3961 ggggtctgct cgaagatctc accgccgagc ctctcgacgg ccctgcctag cccgcgcgcg
     4021 agccgcgccg gatggatggt cgcgcaatgc gggttgtaca gggcgcccct ggcgcccgtg
     4081 atcctgaccc gccctgccat ctcctcggcg tcgagcaaac ggagaccctc ctccaggcca
     4141 agcgcacgga gcgattcgta ggcctcctct atgccaggta gctggtgctc tccgcgggcg
     4201 acgcggagct gcccgccgtg gaagtactgc gcgtcgatct cctcggccgc tgcgacttta
     4261 ccgacctcct cgaccgcgtc tctcatctcc agcagaagcc cccgcgtggc agctcttccg
     4321 aagcgtcgct gcaggtcacc agggctgacg gggaaggccg agttgcacca ggcgccgtta
     4381 cgccccgacg ccccgaagcc ggagatctcc gcctctagta tcaccacgcg taacgaaggt
     4441 tctttcttta gcaggtagta ggccgtccac aggcccgtga aacccgcacc cagaatagcc
     4501 acgtcggcct ccatcgggcc gggcagcgaa gacctggggg cgagctcctc ccctgccgtc
     4561 tccagccaga agctgtagga acggtaatcc ttacccccgg cgcgggcggt cctacttgcg
     4621 ctgacggcgc gggtcaagcc ctaactccgg tagccgagct ctcgttcgag ttcagtgagg
     4681 gcatcgtccg tgatacctgc tatcttcgag atgatctcct tgtctgctac gagcgccggg
     4741 gagaactgga tgacagggtc ttccttgtcg tcgaagcggc agatgagccc gccctctaaa
     4801 agcttcctgg agacgacgcc cttgaagtac ttctgatact cgtcgccttc gagcggcgtc
     4861 ccgtcctccc gctcggggtt gatctccaca gcccagaaga accccatccc cctgacctct
     4921 ttgacgatgg gatggtcggc ggccatccgc ctcagctcgt cgccgaagtg cccttcgagg
     4981 tcgtggacgt tctggagcag cccctcgcgc tcgatgatgt ttatgttctc gatggcgacc
     5041 gcgctggaga caggatgccc cccgaaggtc gagccgtgca tgaacatcgg ggctttgctc
     5101 ctc
//
LOCUS       DATAIR010000309         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_388882, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000309 DATAIR010000000
VERSION     DATAIR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388882"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VHM69_07555"
     CDS             <1..192
                     /locus_tag="VHM69_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2740288.1"
                     /translation="VVTACTKCNARKGNRLPMECGMYPKSTPKEPRYVVMVWYSRDLN
                     EVQRRYVDQFYGQGSLTAS"
     gene            423..1376
                     /locus_tag="VHM69_07560"
     CDS             423..1376
                     /locus_tag="VHM69_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2740289.1"
                     /translation="MDHSDFAERVERIVGNVERAVSGKRDGVLLAVVAMLAGGHVLIE
                     DVPGVGKTLLAKSLARSLRAEFRRIQFTPDLLPADVTGLNVYNQGEGDFEFWPGPVFA
                     NVVLADEINRASPKTQSALLEAMEEGSVTVDGVTRHLAQPFCVVATQNPIEHEGTYPL
                     PHAELDRFMIKMHIGYPDEDAETEMLKLSQDPVADLTPVVGVEEFLEMRRLVEEIYTS
                     EAVLRYVVSVTSATREHDEVYLGASPRASRMLLAASRARAAANGRSYCAPDDVKAMAS
                     SVLSHRIIPSASSSEDGIGPSEVTREIVRDILRSVPVTEAV"
     gene            1383..2624
                     /locus_tag="VHM69_07565"
     CDS             1383..2624
                     /locus_tag="VHM69_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2740290.1"
                     /translation="MSGASRLSVRPTARGWQALVAGAVTLFVARLIGTTQFHQLAYAL
                     LALPIASLVLGVMASRGLRYSRALPPGTRITAGSTAKLRLLLERTRFGATKASVVDSL
                     PEPRSFEFSETREKSRIEVPINFARRGVYELGPAEIRVADPFGLLRFSRRFWERTEVV
                     VYPKVYELRGFPFRGGNVEAGARGARGRRGEEFANLREYRRGDDRRHIHWKSLARTGE
                     LFVKEFSLEAPRRHTVALDLRREGIRTQNNEVEDAVSTTASVLTHLVRDGLPFRLLSA
                     GSGVADTGFGSDEDAYWEAMRILATARVDGGRPLGEIVLGERGGLGEGVVLVSRTRDD
                     ELPHCVRKLREAGISVIVVLLATYTYRAHPGTGGAAQGREAEFLRSVGRLEAAGATVR
                     VVGHPAGVAGLSGARGVETLR"
     gene            2621..>3460
                     /locus_tag="VHM69_07570"
     CDS             2621..>3460
                     /locus_tag="VHM69_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740291.1"
                     /translation="MSGAGTKWWLYGAALAAGSAFSILFTGETYDGSDGGFGGLSVVA
                     IIAGYSAFLPLLFAAVVAALVGSAGRWRFMLLFPTIVVYTLIAVYGRVPLSVSGWREF
                     FMVVGGDVYAAANTMYAEPIPYDLAPGLFVVLVPLVMILVAFATSATLFEESPVISIA
                     VLGLTIGVLSTISFEDGAGPFFAVFLVCTVGLLLSSSQPGPSGPGRPVIVAAALVIAF
                     VLFVPRMPLSDLTVSTGMIDWTRIGTGGTSRLDVQADVGDYLTAGRDAELLRIQSSEP
                     LLWR"
BASE COUNT          547 a         1075 c         1194 g          644 t
ORIGIN      
        1 gtggtcacgg cgtgcaccaa gtgcaacgcg aggaagggca accgactgcc gatggagtgc
       61 ggcatgtacc caaagagtac gcccaaagag ccgcgctacg tcgtgatggt atggtactcg
      121 cgcgacctca acgaggtcca gcgccgatac gtggatcagt tctacggaca ggggagcctg
      181 acggcgtcgt agctttcagc ggtcagcttt cagcggtcag ctttcagcat tcagtaatca
      241 gccctgttgg cggtcgtggt gtgcgatgct tggcgttgtg gatagtggac gcatttcgcc
      301 ggaagaggac gtcgcgttgt gcggaaggga tggccgaagg gttcttgctg acggctgaat
      361 gctgaccgct gacggctata ggctataatt ccctgcgtca cgcgaacata gacaggactc
      421 ctttggacca tagtgatttt gcggagaggg tcgaacgcat cgtcgggaac gtcgagcgtg
      481 ctgtttcagg caaacgcgat ggtgttttgc ttgccgtggt ggcgatgctc gcgggaggac
      541 acgtccttat agaggatgtc cccggtgtgg gcaagacgct tctggccaag tcgctcgccc
      601 gttccttgcg ggcagagttc cgcaggattc agttcacacc cgacctgttg cctgccgacg
      661 tgacgggcct caacgtctac aaccagggcg agggggactt cgagttctgg cccggtcctg
      721 tcttcgccaa cgtggtgctc gcggatgaga tcaaccgcgc cagccccaag acccagagcg
      781 cgctgctgga ggcgatggag gagggttccg tgacggttga cggggtgacc cgccacctcg
      841 cccagccctt ctgtgtcgtc gcgacccaga accccatcga gcacgagggt acctacccct
      901 tgccgcacgc cgagctggac cggttcatga tcaagatgca cataggctac cccgacgagg
      961 acgccgagac cgagatgctc aagctctccc aggaccctgt cgccgacctc actcccgtgg
     1021 tgggagtcga agagttcctc gagatgcgcc gtctcgtcga ggagatctac accagcgagg
     1081 ccgtcctgag atacgtggtc tccgtcacct ccgcgacccg cgagcacgat gaggtatacc
     1141 tcggcgcctc cccgcgcgcc agccggatgc ttctcgccgc ctccagggcg cgggcggcgg
     1201 cgaacggacg ctcgtattgc gcacccgacg acgtcaaggc catggcttcg agcgtgctct
     1261 cgcacaggat cataccctcg gccagcagct ccgaggacgg aattggtccg agcgaagtca
     1321 cccgtgagat cgtgcgggac atcctgcgct cggtgccggt gaccgaggcg gtgtaagaac
     1381 ccttgtcggg cgcgtcgcgt ctctccgtca ggccgacggc ccgcggctgg caggcgctgg
     1441 tggcgggggc cgtcacgctg ttcgtggcgc gcctgatcgg tacgacccag ttccaccagc
     1501 tcgcctacgc gctccttgct ctgccgatag cgtccctggt cctcggtgtg atggcctcca
     1561 ggggcctgcg ttactcgcgc gccttgcctc ccggtacccg catcacggcg ggcagtaccg
     1621 cgaagttgcg tctcctgctc gaacgcacgc gtttcggcgc cacgaaggcg agcgtggtgg
     1681 acagtctccc ggagccacgc agcttcgagt tctccgagac gagggagaaa agcaggatcg
     1741 aggtccccat caacttcgcc cgcagggggg tctacgagct gggtccggcc gagatcaggg
     1801 tggccgaccc tttcgggctc ctccgtttct cgagaaggtt ctgggagagg accgaggttg
     1861 tcgtctaccc gaaggtctac gagctgcgag gcttcccctt ccggggcggg aacgtcgagg
     1921 ctggtgccag gggggcgcgt ggccggcgag gtgaggagtt cgcgaacctg cgggagtacc
     1981 gcagggggga cgacaggcgg cacatccact ggaagagcct ggcgcgcaca ggtgagcttt
     2041 tcgtcaagga gttctccctg gaggccccga ggcgccacac cgttgccctg gacctgcgca
     2101 gggaggggat aaggacccag aataacgagg tcgaggacgc cgtctcgacg acggcctccg
     2161 tgctcacgca cctcgtaagg gatggtctgc ctttccggct tctgagcgcg ggtagtggtg
     2221 tcgccgacac gggtttcgga tccgacgaag acgcctactg ggaggcgatg aggatccttg
     2281 cgacggcccg cgtcgacggg gggcgtcccc tcggggagat cgtgctcggg gagaggggag
     2341 ggctcgggga gggcgttgtc ctcgtctcgc gcacccgcga cgatgagcta ccccattgcg
     2401 tccgcaagct cagggaggct ggcatatcgg tgatcgtggt actcctcgcg acctacacgt
     2461 acagggcgca ccccggaaca gggggcgccg cacagggccg cgaggcggag ttcttgagga
     2521 gcgtcgggag gctggaggcg gccggggcaa cggtgcgcgt cgtgggccac cctgcggggg
     2581 tggcagggct ctccggcgct cggggggtcg agacgttgcg gtgagcggag cagggacgaa
     2641 atggtggctc tacggggcgg cgctcgcggc aggcagcgcc ttcagcatcc tgtttacggg
     2701 cgagacctac gacggttccg acgggggttt cggcgggctc tcggtggtcg cgatcatcgc
     2761 agggtattca gccttcctgc cactgctctt cgccgcggtg gtggctgcgc tggttggctc
     2821 ggcgggccgg tggcggttca tgctcctctt ccccacgata gtcgtgtaca cgctgatcgc
     2881 cgtgtacggg agggttccgc tctcggtctc gggatggcgc gagttcttca tggtcgtagg
     2941 gggcgatgtc tacgctgcgg cgaacaccat gtacgccgag ccgataccct acgacctcgc
     3001 gcccgggctc ttcgtcgtgc tcgtgcccct ggtcatgatc ctcgtggcct tcgccacctc
     3061 ggcgaccctc ttcgaggaga gccccgtcat ctctattgcc gttctgggac taaccatagg
     3121 cgtgctctcg accatctcct tcgaggacgg ggcaggacct ttcttcgccg tcttccttgt
     3181 ctgcacggtt ggactccttc tctcctcgag ccagccagga ccatcggggc ctggccgtcc
     3241 tgtaatagtc gctgcggcgc tcgtgatcgc gttcgtgctc ttcgtgccca ggatgcccct
     3301 ctcggacctg accgtcagca cgggcatgat agattggacg cgcatcggga cgggcggcac
     3361 ctccaggctc gacgtgcagg ccgacgtcgg agattacctc acggcgggga gggacgccga
     3421 gctcctgcgg atacagagct ccgagccgct cctgtggcgc
//
LOCUS       DATAIR010000310         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_112290, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000310 DATAIR010000000
VERSION     DATAIR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112290"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VHM69_07575"
     CDS             complement(<1..848)
                     /locus_tag="VHM69_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX2740292.1"
                     /translation="MYRIRINERCEVSALDIGLVAAVAMLGPFWAAVAALPCAVVVGR
                     RDWLRTLYEASRNSVQVFLAGIVFSTVAEPLLEKDPVLTAPVVYATFASGVVLLAVNH
                     ATDAGLLKVKYQRGLGIFWREQVEPYLLADGVNVLTAGLGVLALLEVSPVAALVVIAG
                     SVGSQVLAYRSREQMKENSELQARVVSLEGALATSNATFGTMIIRDLGQRDGYTHRHA
                     AATARYAADLAREMKLDETHAERLRMAGLLHNIGLFGLPEELLVTTGRLNSIARSHLD
                     EHPARGA"
     gene            complement(1352..>2606)
                     /locus_tag="VHM69_07580"
     CDS             complement(1352..>2606)
                     /locus_tag="VHM69_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740293.1"
                     /translation="APVAGRIFDRVRHDLAPGVGGMDSTNDSLLKNVRFMRLWIGQGT
                     SFLGDAVSMVALVVLVFQITGSASAVGGALVARLVPTIASPLAGVLADRVDRRMVLVA
                     SDLARAVLILGMVFTRDLATIYVLAFLMGLARTLFNPTIRAAFPGVVGGGDLTRANAL
                     ISGTFSVAETAGPALGGLLVATVGVEAAFILDAATYLISAALLSLNPLPRPQREDDDA
                     GFGEDLKAGFAYLAGARVPLAMVAGGFLTIVTINITIPAEVFLAKETFGAGDAGYGLL
                     VGIFGGGMVLGSAMMAALGDRVRLLPLYFLGIFISALALVGTGLSPAFVIALGALMAA
                     GFANGMENVTTDTILQKRVPDAFLGRVFSVRYLGFSVGEILAYPLGGAIVDAAGARFT
                     YLLAGAATAVAGLAVIIYLGLSTDA"
BASE COUNT          565 a          831 c          757 g          453 t
ORIGIN      
        1 gcgccgcgtg cgggatgctc gtcgagatga ctcctggcga tggagttgag cctgcctgtc
       61 gtcaccagca actcctcagg caacccgaac aacccgatgt tgtgcaagag accggccatc
      121 cgcaaccttt cggcgtgggt ctcgtccagc ttcatctcgc gggcaaggtc agcagcatac
      181 ctggcagtgg cggcagcgtg cctgtgcgta tacccgtccc tctggccgag gtcccggatg
      241 atcatggtcc cgaacgtagc attggacgtc gcgagagccc cttctagaga cactactcgc
      301 gcctgcaact cgctattctc tttcatctgc tcacgcgagc ggtaagcgag tacctggctc
      361 ccaacagagc cagcgatcac gaccagcgcc gcaacgggac tgacctcgag taaggccaac
      421 acacctagcc ccgctgtgag cacgttgaca ccgtcggcaa gcaggtacgg ttctacttgc
      481 tctctccaaa agattccaag ccctcgctgg tacttcacct tcagtaggcc ggcgtcggtc
      541 gcatggttga ccgccagcaa gaccacccca gaagcaaagg ttgcgtatac gaccggggcc
      601 gtaagtacag ggtctttctc cagcaggggc tcagcgacgg tggagaatac tatgcctgcc
      661 aggaatacct gcacggagtt gcgggacgcc tcgtagagcg tgcggagcca gtccctcctt
      721 ccgacgacta cggcgcatgg caaggctgct accgcagccc aaaacggccc aagcatcgct
      781 accgccgcca cgagccctat gtccagggcg ctgacctcac atcgctcatt aatccgtatg
      841 cgatacatac atgcgataca caagataccg ccgaagacca gcgtgcctaa gataagggaa
      901 gcctctggtg cctcaccgga ccagtaggcc caagacgcag caaatgttat ggcaagagcc
      961 gccaaacctc caacataggt tttcacggca gtttcgccca aaggttcctc taacggtagc
     1021 gtgcgaagcc tacactcata cgtaacgcta tattctgcac gctaattgcg ctttgcacaa
     1081 gcgcaatctg accatttcct accaggtgtg cttctggctg tacatattca gtacctcctc
     1141 tctccgttta gctagtcgtg ctacatcgaa cgtttattgc tcggccctcg cttaccaggt
     1201 gtgcttctga ctaagcatca cttgccacct cccctctgtt gatccttctg atacctggat
     1261 actacctgaa ctaaggtgta acaggaaacg gcgaggtgct agtactcgga accaatactt
     1321 cttagtctgc atcaggcagc atgaacagag gtcaggcgtc ggtagaaaga ccgaggtaaa
     1381 tgatgacggc gagacccgca actgcggttg cggctccggc gagtaggtaa gtaaaacgcg
     1441 ctcccgcggc gtccacgatc gcgcctccca aggggtaggc gaggatctcc ccaacgctga
     1501 accccaagta cctcacggag aagacgcgtc caaggaaagc gtcgggcacc cgcttttgca
     1561 ggatggtgtc cgtggtcacg ttctccatgc cgttggcgaa cccagccgcc atcagcgcgc
     1621 caagcgcgat gacgaacgcg ggagagagac ccgttcccac aagcgccagc gccgagatga
     1681 agatcccgag gaagtagagg ggcagaagcc tgacccggtc cccgagcgcg gccatcatgg
     1741 ccgagccgag caccatgccg cccccgaaga tgccgaccag gagaccgtaa ccggcgtcgc
     1801 cggccccgaa ggtctcctta gcgaggaaca cctcggcagg gatcgtgatg ttgatcgtga
     1861 ctatggtgag aaagcccccg gcgaccatcg cgaggggcac gcgcgccccc gcaaggtagg
     1921 cgaatccggc cttcagatcc tcaccgaaac cggcatcgtc gtcctccctc tgaggtcgcg
     1981 ggagggggtt gagagagagc agggcggcgg agatcaggta ggttgcggcg tctagaatga
     2041 aggccgcttc gacgcccacg gtggccacca gaaggccccc tagcgccggg cccgccgtct
     2101 ccgcgacgct gaacgtaccg ctgataaggg cgttggcccg cgtgagatcc cctcctccca
     2161 ccacgcccgg gaaggcggcc ctgatagtag ggttgaacaa ggtgcgtgcc aggcccataa
     2221 ggaaggccag tacgtagatg gtcgcgaggt ccctggtgaa gaccatcccc agaatgagaa
     2281 ccgccctcgc gaggtcgctc gctaccagga ccatcctgcg gtcgacgcgg tccgcgagga
     2341 ccccggctaa ggggctcgcg atggtaggca cgagccgggc gacgagcgcg ccgcccaccg
     2401 ccgaggcgct gcccgtgatc tggaaaacaa ggacaacgag cgcaaccatg gagacggcat
     2461 ctcctaagaa agatgtcccc tggcctatcc agagccgcat gaagcgaacg ttttttagaa
     2521 gtgagtcgtt tgtggagtcc atgcccccaa ctcctggagc gagatcgtgc ctgacccggt
     2581 cgaatatcct cccggcgacc ggagcc
//
LOCUS       DATAIR010000311         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20032, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000311 DATAIR010000000
VERSION     DATAIR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20032"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VHM69_07585"
     CDS             complement(<1..1033)
                     /locus_tag="VHM69_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX2740294.1"
                     /translation="MTILNESGISDVLPPLHAVSGLAERLPGVLVVVAGTQAEAHLVQ
                     SLPAAPSPNLPPYPRNQRVILAVLDPDDPPQQGLLASRIVETAAGIRGTKLVLLVAGR
                     TAESHGIDAAFEATLVARKLDLPAEVVNPDAPYEVPGTLSTDLEDRALAALVNLCPKR
                     KTSDLVETAPQAKRGGLFGGLLGRGREASESSQARPVVLLGGTFSPGAARGLTSELSR
                     AGVGVTGAVPGSGIGELPPLGEGTVVAVMDPNLTTAAAEAERRGATVVRTLMPVGVDG
                     TARFVQDVAEEAGSRISEVVRARSAWEDLGALRNRIRGKRIFFAGDTGMEIPLARFLA
                     DAGAVVLEVG"
     gene            complement(1095..1808)
                     /locus_tag="VHM69_07590"
     CDS             complement(1095..1808)
                     /locus_tag="VHM69_07590"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HEX2740295.1"
                     /translation="MSPVHVRAEPGDFAESVLLPGDPLRAKYIAKNFFEEARLVTEER
                     GLLGYTGTYEGKPVSVQATGMGCPSAAIVTEEMIQLGAKNLLRVGTCGGYSGEMSLGD
                     LIVATSATPQDGTVSSLTRGVSYAPAAHFDLVHAAHHAAEGAGRRTFVGPIVSSDLFY
                     DPTEDPAKLWSGLGVLAVEMEAAAIFTIAAMHGVRAGCLLTVSDTIGAEVVRISDEEL
                     RNAVDNMMALALDTLHALN"
BASE COUNT          341 a          698 c          635 g          383 t
ORIGIN      
        1 cgccgacctc cagtaccaca gcccccgcgt cggcgaggaa acgggcgagc gggatctcca
       61 tccccgtgtc gccggcgaag aagatcctct ttcccctgat gcggtttctc aaggccccaa
      121 ggtcctccca tgctgacctg gcccgcacga cctcgctgat ccgcgacccc gcctcctcgg
      181 ccacgtcctg cacgaagcgg gccgtgccgt ccacgccgac gggcatgagg gtcctgacaa
      241 cagtggctcc ccgcctctcg gcctcagcgg ccgccgtcgt caggttcggg tccatcaccg
      301 cgaccaccgt gccctctccc agtggcggga gctccccgat gcccgacccc ggcacggccc
      361 ctgtcacccc caccccagcc cttgagagct ccgatgtgag tcctcgcgca gcacctggtg
      421 agaaagtacc gccgagcaga accacgggac gggcctggct cgactctgac gcttcgcgtc
      481 ctcgtcccag caggccgccg aacagcccgc cgcgcttcgc ctgaggtgca gtctccacga
      541 ggtctgaggt cttcctcttc gggcagaggt tcaccagggc cgcgagggca cggtcctcga
      601 ggtcggtgga gagggtgccg gggacttcgt aaggggcgtc agggttcacg acctccgcgg
      661 gcaggtccag cttgcgggcg acgagcgtgg cctcgaacgc ggcatctatg ccgtgagatt
      721 ccgcggtccg tccggcgacg agcagcacga gcttcgtgcc gcggataccc gcggcggtct
      781 ccacgatgcg ggaggcgagc agaccctgtt gcggcgggtc gtcagggtcg aggaccgcga
      841 ggatcacccg ctggttacgc gggtaagggg ggaggttcgg agaaggcgcg gcgggtaggg
      901 attggaccag atgagcttca gcttgcgtac ctgcgacgac cacgagtaca ccaggaagcc
      961 tctctgccag gccacttacg gcatgcaaag gggggagaac gtccgatatc cccgactcgt
     1021 tgaggatcgt caatctactt tcctatctag cggaggccag tccacacgac ccgcctcgtc
     1081 cccacatgag gttcctagtt taaggcgtga agggtatcca gcgctagtgc catcatgtta
     1141 tccactgcgt tacgcagttc ttcgtcgctg atccttacga cttccgcgcc gatggtgtcg
     1201 gagaccgtca acaaacatcc cgccctcaca ccgtgcatcg ctgctatggt gaagatcgcc
     1261 gctgcctcca tctcgacggc gagcaccccg aggccgctcc acagctttgc cgggtcttcg
     1321 gtggggtcgt agaagaggtc cgagctcact attgggccga cgaaggtcct gcgtcccgcg
     1381 ccctctgcgg cgtggtgggc ggcgtggacg aggtcgaagt gggccgcagg ggcgtacgaa
     1441 acgccgcgtg tgagcgagga gaccgtccca tcctgcggcg tcgctgaggt cgcgacgatc
     1501 aggtccccca gactcatctc cccgctgtat cccccgcagg tacccaccct gagcaggttc
     1561 ttcgccccga gctgtatcat ctcctcagtc acgatggcgg cgctcgggca tcccatccca
     1621 gtggcctgga cggagaccgg tttcccctca taagtgcccg tgtacccgag caaaccgcgc
     1681 tcttccgtca ccagcctcgc ttcttcgaag aagttcttgg cgatgtattt cgccctcaaa
     1741 gggtccccgg gcaagagaac gctctccgca aaatctccag gctccgcacg cacatgaaca
     1801 gggctcatga tcgactccct tcggtcgtct caaggcttca ggtatcaggt ttcagccgtc
     1861 aggttctttg ctgtgcctgt aacctgaagc ctgataccta cttctcacag gctgtccagg
     1921 atagcagggg gaggctctat gggctcgtcg gagatttcta gcgcctcgag aacgaggcgg
     1981 ccggctcgct ccgcttcgcg ttcgccgtag aggtgggcta tgggttggcc ctcttcgatg
     2041 tggtctccag gctttat
//
LOCUS       DATAIR010000312         5871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_55181, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000312 DATAIR010000000
VERSION     DATAIR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55181"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VHM69_07595"
     CDS             complement(<1..1088)
                     /locus_tag="VHM69_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2740296.1"
                     /translation="MAVQTARKIPLKRVRNIGIMAHIDAGKTTTTERILLYTGLTHKL
                     GEVHDGNAVMDWMAQERERGITITSAATTVFWGGIEGSSKNGKGSREGVHSRIADQHR
                     VNIIDTPGHVDFTVEVERSLRVLDGSIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYKAVGTMVDRLGANAVPVQLPIGAESEFQGIVDLVEMRAIVYTDDLGA
                     TWEVTDIPEEMRGLADEYREKLLEAVADQDEELMVMYLEGEEIEPDQIRAAIRKATLN
                     IQMTPVFVGSAFKNKGVQPLLDGVVDYLPSPLDVPPVEGKTLDGEEITREADENGPLA
                     ALAFKVQADPHVGKLTYMRVYSGTLKAGS"
     gene            complement(1101..1571)
                     /gene="rpsG"
                     /locus_tag="VHM69_07600"
     CDS             complement(1101..1571)
                     /gene="rpsG"
                     /locus_tag="VHM69_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2740297.1"
                     /translation="MPRRAAPPKRKITGDPVYGSVLVQQLVNKIMLDGKKSTSEKIVY
                     DALSYIEERTGTSPVQVLNNALDNIRPRLEVKSRRVGGATYQVPVEVDARRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
     gene            complement(1583..1951)
                     /gene="rpsL"
                     /locus_tag="VHM69_07605"
     CDS             complement(1583..1951)
                     /gene="rpsL"
                     /locus_tag="VHM69_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX2740298.1"
                     /translation="MPTTNQLVRNEREKQTKKAATPALQGSPQRRGVCTRVSTTTPRK
                     PNSALRKVARVKLTNGQEVTAYVPGEGHNLQEHSVVLVRGGQVKDLPGVRYKVVRGAL
                     DTLGVNNRKQGRSKYGTKKS"
     gene            complement(2283..>5871)
                     /locus_tag="VHM69_07610"
     CDS             complement(2283..>5871)
                     /locus_tag="VHM69_07610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX2740299.1"
                     /translation="IELAAPVAHVWFVKGVPSRMGYLLDISPKELDRVLYFASSIVTW
                     VDREERANDIDLLRGRVEDEIQQLEADRDEEIDQIQALRTKRESVIKGESSPDELTEE
                     YADIPEEDRESAIAKVHKEAEEAVADIGRSVTEQIGLVRRAWEEFQTLEPRQIVDDEE
                     LFREMKDRFGDEYGYGVYFRGGMGAEAIRDLISQVDLEDEAQSLRDVVASSKGQKRQK
                     AIKRLKVVDAFRTSGNGPEWMIMEAIPVLPPDLRPMVQLDGGRFATSDLNDLYRRVIN
                     RNNRLRRLLDLGAPDVIVNNEKRMLQEAVDALFDNGRRGRAVTGAGNRALKSLSDMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPGLKMHQCGLPRLMAVELFKPFVMKVLVDRAL
                     APNIRSAKQMVERLRPEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQ
                     VHPLVCSAFNADFDGDQMAVHVPLSPEAQAEARVLMLSTQNILLPANGFPIAVPSQDM
                     VLGLYYITYGGAEGFEDAEVKALISSYDEAEAAYKEGTLKIHDKVITRRNGERFETTL
                     GRVLFNENVEQTLRDYLGDSYDPASYPYINHTLKKGEIKDLVAEYVDRYPTELVSQLL
                     DTLKRVGFHTATKAGISVGKNDIVVPEQKGEILERYQHMVDEIEDQWDQGFISDEERL
                     ERVIGLWTQAKNEVEQAMKDNLWRLNPIYMMADSGARGNYNNFTQLAGMRGLMTNTKG
                     EIIDEPVKSNFMEGLSVLEYFTSTHGARKGQADTALRTADSGYLTRRLVDVSQDVVVN
                     DEDCGSDGYVDLPLFLEGGKGDPNDSVAARFLARDLVNPETGEVVAEAGTDITPRLLA
                     SWREELPRETLVSVRTPSKCESPHGVCQTCYGRALSTYRPVDVGEAVGIIAAQSIGEP
                     GTQLTMRTFHTGGIAGEDITAGLPRVVELFEARHPKAEATLAEIDGIVSLEEADSDRM
                     IKVVITAPDNSEVREYKVERQLLRPQFVEGAEITANTPITEGSVYPHAILENDLRYDR
                     GSTTTERYLVDEVQKVYRAQGVDIHDKHIEVIVRQMLRKVVVDDPGDTKLLPEQVADR
                     FTVLAENARVEEEEGRIATFHTVLMGITKASLATDSFLSAASFQETARVLTDAAIEGK
                     VDGLAGLKENVIIGKLIPAATGLPRYRQLTVAVEGYKAQEVDELDIAAS"
BASE COUNT          949 a         1911 c         1773 g         1238 t
ORIGIN      
        1 gagccggcct tgagcgtgcc cgagtacacc cgcatgtacg tcagcttccc gacgtgcggg
       61 tcggcctgca ccttgaaggc gagcgccgcc agcggaccgt tctcgtcggc ctcgcgggtg
      121 atctcctctc cgtcgagagt cttgccctcc acgggcggca cgtcgagcgg gctcggcagg
      181 tagtcaacga cgccgtccag cagcggctgc acgcccttgt tcttgaaagc agatccaacg
      241 aacacagggg tcatctggat gttcagggtc gcctttcgga tggcggcgcg gatctggtcc
      301 ggctctatct cctcaccctc cagatacatg accatgagct cctcgtcctg atcggcgacc
      361 gcctcgagaa gcttctcccg atactcgtcg gcgagcccac gcatctcctc cggtatgtcc
      421 gtgacctccc acgtcgcacc aaggtcgtcc gtgtagacga tggccctcat ctcgacgagg
      481 tcgacaatac cctggaactc actctcagca cctatcggca actgcactgg taccgcgttc
      541 gcccccagcc tgtcaaccat cgtgcccaca gccttgtaga agtcggcccc gatgcggtcc
      601 atcttgttga cgaacgctat gcgcggcacg ccgtacttgt cagcctgcct ccacaccgtc
      661 tcggattgcg gctccacgcc agcaaccgag tcgaacaacg cgatcgaacc gtcgagcacg
      721 cgtagactgc gctccacctc tactgtgaag tccacgtgtc cgggcgtgtc tataatattg
      781 acgcgatgtt gatcggcgat acgggaatgg acgccctccc gggagccctt accgttcttg
      841 cttgagcctt cgataccgcc ccagaagacc gttgttgcgg cggaagtgat cgtaatgccg
      901 cgctcgcgct cctgggccat ccagtccata acggcgttac cgtcgtggac ctcacccagt
      961 ttatgggtca ggcctgtata gagcaggata cgctcggtcg tcgtggtctt accggcatcg
     1021 atgtgggcca tgatgccgat attcctgact cgttttaatg ggatctttct cgcagtttgt
     1081 acagccataa tagttcctcg tcaccacctg taatgggcga aagcccggtt ggcctcggcc
     1141 atccgatgcg tgtcttcctt acgcttcacg ctagagccga tgttgtcctt ggcgtccagg
     1201 atctcccctg ccagcctgcg gcccatcgtc ttctcgcgcc tggcgcgggc gaagccgacc
     1261 atccagcgca gcgccagcgt gttggccctg cgggcgtcga cctcgacggg cacctggtag
     1321 gtggcgccgc cgacccggcg gctcttaact tccaggcgag gtctgatgtt gtccagggcg
     1381 ttgtttagta cctggacggg gctcgtgccc gtgcgctcct caatatacga gagggcgtcg
     1441 tagacgatct tctcggaggt gctcttctta ccgtcgagca tgatcttgtt gaccaactgc
     1501 tgcaccagca cgctcccgta gacgggatct ccggtgatct tccttttcgg tggcgctgct
     1561 cgcctcggca tctcctatcc tcctacgact tctttgtccc gtacttggag cgtccctgct
     1621 tgcggttgtt caccccgagg gtgtcgaggg caccgcgcac tactttgtag cgaacgcctg
     1681 gcaggtcctt gacctgtccg ccgcgaacca gcaccacgga gtgctcctga aggttgtgtc
     1741 cctctccagg aacgtatgcc gtaacctctt gtccgttagt cagcttcaca cgcgcgacct
     1801 tgcgcagcgc ggagttcggc ttcctcggcg tcgtcgtcga gacgcgcgtg cagacgccgc
     1861 gacgctgggg cgaaccctgc agggccggcg tcgccgcctt cttcgtctgt ttctcacgct
     1921 cattgcgcac aagctgattg gtcgtcggca tggacgcgcc tctctctcta cacctggaaa
     1981 aggggactac tactacaaag tcgtgcgccg cggcacgaag accgattacc ctattggcaa
     2041 ccgctcgtaa ccgcgacgca ttattttagg ctcaggaagc tgctatgtca agctgaaaga
     2101 gctatcagct tttagccatc agcaatcagc atttttcctc ccaccgatgg cctctccggc
     2161 cggtttcggc cttcctgcac tgtggtttaa gtcgtggaac tcgtccaacc gcttcgctct
     2221 gtccaaaagt ggtcagcccc cagcaacaga agctgaaggc tgtccgctga ttgctgacgg
     2281 ctctaagaag ccgctatgtc aagctcgtca acttcctgtg ccttgtatcc ctcgacggct
     2341 acggtgagct gcctgtagcg cggcagtcca gtagccgcgg ggatgagctt accgatgatc
     2401 acgttctcct tcaggcctgc gaggccgtcc accttgccct cgatggccgc gtctgtcagg
     2461 acacgcgcgg tctcctggaa cgaggctgcg gagaggaagc tgtcggttgc gagcgaggcc
     2521 ttggttatgc ccatcagcac cgtgtggaag gtggctatcc ggccttcctc ctcctcgacg
     2581 cgcgcgttct cggcaagcac cgtgaaacgg tcggcgacct gctcggggag cagcttcgta
     2641 tcgccggggt cgtcaacgac caccttgcgg agcatctggc gtacgatcac ctcgatgtgc
     2701 ttgtcatgga tgtccacgcc ctgcgcgcgg tagaccttct gcacctcgtc caccaggtag
     2761 cgctcggtgg tggtgctgcc acggtcgtag cggaggtcgt tctcgaggat cgcgtgcggg
     2821 tagacggacc cctccgtgat cggggtgttc gccgtgatct cggcgccctc gacgaactgg
     2881 ggcctgagga gctggcgctc gaccttgtac tcgcgtacct cggagttatc cggcgcggtg
     2941 atgacgacct tgatcatacg gtccgagtca gcctcctcca gggagacgat gccgtcgatc
     3001 tcggcgagag tcgcctctgc cttggggtgc cttgcctcga agagctcgac gaccctcggg
     3061 aggcctgccg ttatgtcctc gccggcgatg ccgccggtgt ggaaggtacg catcgtgagc
     3121 tgcgtgccag gctcgccgat ggactgcgcc gcgatgatgc cgacggcctc gcccacatcg
     3181 acgggcctgt aggtcgagag cgcccgaccg taacaggtct ggcacacgcc gtgcgggctc
     3241 tcgcacttcg agggcgtccg cacggagacg agagtctcgc gcggcaactc ctcacgccag
     3301 gaggcgagca ggcgcggcgt gatatccgta ccggcctctg cgacgacctc gcctgtctca
     3361 gggttcacca ggtcgcgggc aaggaagcgt gcggcgacgg agtcgttcgg gtcgcccttg
     3421 ccgccttcga ggaagagcgg caggtcaacg tagccgtccg agccgcaatc ctcgtcgttc
     3481 accacgacat cctgggagac gtcgacgagg cggcgggtca ggtagcccga gtcggcggta
     3541 cgaagcgccg tgtcggcctg gcccttcctg gcgccgtggg tcgatgtgaa gtactccagt
     3601 accgagaggc cctccatgaa gttggacttc acgggctcgt cgatgatctc acccttcgtg
     3661 ttagtcatga ggccgcgcat accggcgagc tgggtgaagt tgttgtagtt accacgggcg
     3721 cccgagtcgg ccatcatgta gatcgggttc aggcgccaga ggttgtcctt catcgcctgc
     3781 tcgacctcgt tcttggcctg ggtccagaga cctatgacac gctccaggcg ctcctcgtcg
     3841 gagatgaaac cctggtccca ctggtcctcg atctcgtcga ccatgtgctg gtagcgctcg
     3901 aggatctccc ccttctgctc aggcacgacg atgtcgttct tgccgacgga gataccggcc
     3961 ttggtggccg tgtggaaacc gaccctcttc agggtatcga ggagctggct gaccagctcc
     4021 gtcgggtagc ggtcaacgta ttcagcgacg aggtccttga tctcaccctt cttcaacgtg
     4081 tggttgatgt aagggtagga cgccggatcg taggagtcgc cgaggtagtc cctcagggtc
     4141 tgctcgacgt tctcgttgaa aagaacgcgc ccgagcgtgg tctcgaaacg ctccccgttg
     4201 cgacgggtga tcaccttgtc gtggatcttg agcgtcccct ccttgtacgc ggcctcggcc
     4261 tcatcgtagg aggagattag cgccttgacc tcggcgtctt cgaagccctc ggccccaccg
     4321 tatgtgatgt agtagagccc gagaaccatg tcctgcgagg gaacggcgat cgggaacccg
     4381 ttcgccggca gcagtatgtt ctgcgtagag agcatgagga cgcgggcctc ggcctgcgcc
     4441 tcggggctca gtggaacgtg cacggccatc tggtcgccgt cgaagtccgc gttgaacgcg
     4501 gagcagacga gcgggtggac ctggatggcc ttaccctcga cgagcacggg ctcgaaagcc
     4561 tggatgccga ggcggtgaag cgtcggtgcg cggttcagga gcacggggtg ctccttgatc
     4621 acgtcctcaa ggacgtccca tacctcaggc ctcagccgct cgaccatctg cttggcgctc
     4681 ctgatgttcg gcgcgagcgc ccggtcaaca aggaccttca taacgaaagg cttgaagagc
     4741 tcgaccgcca tcaaacgagg caggccgcac tggtgcatct tcaaaccagg accgacaacg
     4801 atcaccgacc gtccggagta gtcgacgcgc ttgccgagca ggttctgcct gaagcgcccc
     4861 tgcttgccct tcagcatgtc agagaggctc ttcaatgcac ggttgcctgc gccggttacg
     4921 gcgcggcccc ttcttccgtt atcgaagagg gcgtcgacgg cctcctggag catccgcttc
     4981 tcgttgttca cgatcacgtc cggcgccccg aggtcgagca gacggcgaag cctgttgttc
     5041 ctgtttatga cgcgccgata gagatcgttg agatcactcg tcgcgaagcg tccgccgtcg
     5101 agctggacca tcggacggag gtcgggcggg agtaccggga tggcctccat gatcatccac
     5161 tccgggccgt tgccgctggt gcggaaggcg tcgacgacct tgaggcgctt gatcgccttc
     5221 tggcgcttct gtcccttgga ggaggccacg acatcgcgca ggctctgggc ctcatcttcg
     5281 aggtcgacct ggctaatgag gtcccttatc gcctcggctc ccatgccgcc gcggaagtac
     5341 acgccgtagc cgtactcgtc gccgaaacgg tccttcatct cgcggaagag ctcctcgtcg
     5401 tccacgatct ggcgcggctc gagcgtctgg aactcctccc acgcacgcct gacgagccct
     5461 atctgctcgg tcacggagcg ccctatgtcc gcgacggcct cctcggcctc cttgtggacc
     5521 ttcgctatgg cggactcgcg gtcctcctcg ggtatgtcgg cgtactcctc cgtcaactcg
     5581 tcgggcgagc tctcgccctt tatcacgctc tcgcgcttcg tcctgagggc ctggatctgg
     5641 tcgatctcct cgtccctgtc ggcctcgagc tgctggattt cgtcctctac cctgcccctg
     5701 agcaggtcaa tgtcgttggc ccgctcctca cggtctaccc acgtgacgat ggagctggcg
     5761 aagtacagta cccggtcaag ctccttcggg cttatgtcga gcagatagcc catccggctc
     5821 ggcacgccct ttacgaacca cacgtgcgcc actggtgcgg cgagctcgat g
//
LOCUS       DATAIR010000313         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_147926, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000313 DATAIR010000000
VERSION     DATAIR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147926"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VHM69_07615"
     CDS             complement(<1..351)
                     /locus_tag="VHM69_07615"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX2740300.1"
                     /translation="MIGIVEERMMEVADLCEKYGVKRLDLFGSAAGDGFDPEVSDLDF
                     VVSFERRDPPGLFNRYFGLEEDLEALFGRSVDLVMEGALQKSRRFAESVEGSRVLLYE
                     ACQRVAEGQALPQPA"
     gene            complement(507..>1601)
                     /locus_tag="VHM69_07620"
     CDS             complement(507..>1601)
                     /locus_tag="VHM69_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX2740301.1"
                     /translation="GSIKIDPYGSLRAPYKIPSGSTYSVISRVPNSSPDQLRSAGTAY
                     PEEILSKYTQLPPTGLERTRSLAARLTEGTTNPYEAVLRMNQYLKTTYPYDLSIAPQG
                     EDMDAVEYFLFEQRRGYCEQFSSSLAVMARSQGIPARVATGYAPGEYNPFTGLYEIKA
                     SDAHAWVEVYFPQYGWTTFDPTPSFDSTPWQYKAAGNLQGGKAFGFIAKRLGEAAGPV
                     AGAAGTLMRGVARLDPVSIIVVGVLVGGAYLAFLYTRKLVVRYRREPLSVRPIKVSDA
                     RLYSRYKAITTALEEAGIAREPQETPEQYARRAAESLAEPRIERLGEIYLHARFRDAV
                     PATLVEEFDNLEPGTHAAIQRLREAQTVRG"
BASE COUNT          241 a          500 c          538 g          322 t
ORIGIN      
        1 cgcgggttgc ggaagggctt gcccttccgc aacccgctgg caggcctcat acagcagcac
       61 ccggctgccc tccacgctct cggcgaagcg gcggctcttc tggagggcgc cctccatcac
      121 cagatctaca ctgcgtccga agagcgcctc caggtcttct tcgagcccga agtaccggtt
      181 gaaaagcccc ggcggatctc gcctctcgaa agacacgacg aagtccaggt cgctaacttc
      241 ggggtcgaac ccgtcacctg cggccgagcc gaagaggtcc agccgcttta cgccgtactt
      301 ctcgcagaga tctgcgacct ccatcatcct ctcctcgacg atgccgatca tttcagcacg
      361 ttccagcctt cggcttccgc tttcagcatt tcagcacgct cagcccttcg ggcttcgctt
      421 gtcagcacgg cgcctttggc gccttgtcag cattttagct tgcttttctg aagcctgatg
      481 cctgtaacct gatgccttag ccgcggtcat ccacggaccg tctgcgcctc ccttagccgc
      541 tgtatagccg catgagttcc tggttccaga ttgtcgaact cctcgaccag ggttgccggg
      601 acggcgtcgc ggaagcgggc gtggaggtag atctcaccta gccgctctat cctcggctcg
      661 gccaggcttt cggcggccct tcgagcgtac tgctcggggg tctcctgtgg ttcgcgggcg
      721 atgcccgctt cctccagggc ggtcgtgatc gccttgtatc tgctgtagag gcgggcgtct
      781 gagaccttga tcgggcgcac ggataggggc tcgcggcggt agcggacgac gagttttctg
      841 gtgtagagga aggccaggta agcaccgccg accagcactc ccacgacgat gatgctcacg
      901 gggtcgaggc gggcgacgcc gcgcatgagc gtccccgcag ccccggccac gggccctgcc
      961 gcctcgccga gcctcttcgc gatgaaaccg aaggccttgc ctccttgcag gtttccggcc
     1021 gccttgtact gccagggtgt ggagtcgaag ctcggggttg ggtcgaaggt cgtccagccg
     1081 tactgcggga agtagacctc gacccaggcg tgggcgtctg aggccttgat ctcgtagagg
     1141 ccggtgaagg ggttgtactc gcctggcgcg tagccggtgg ccaccctggc cggtatcccc
     1201 tgggagcggg ccatgacggc gagcgaggag ctgaactgct cgcagtagcc gcgccgctgc
     1261 tcgaagagga agtactcgac ggcgtccatg tcctccccct ggggcgcgat ggagaggtcg
     1321 taagggtagg tggtcttgag gtactggttc atcctgagca cggcctcgta ggggttggtc
     1381 gtgccctccg tcagcctggc ggcgagagat cgggtgcgtt cgaggcccgt cggggggagc
     1441 tgcgtgtatt tgctcagtat ctcctcgggg taggccgtgc cggcggagcg gagttggtcg
     1501 ggggaggagt tcgggactcg ggagatgacc gagtacgtgg agccagaggg gatcttgtac
     1561 ggcgccctca gggagccgta ggggtcgatc ttgatgctgc c
//
LOCUS       DATAIR010000314         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20060, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000314 DATAIR010000000
VERSION     DATAIR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20060"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VHM69_07625"
     CDS             complement(<1..722)
                     /locus_tag="VHM69_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2740302.1"
                     /translation="MKKSFGGWMRQRKVWAWRRAAVRYPALPAMKRRLSGITLGITRF
                     VSSVRGRSGVADTAALGLDPANITWIFCVGRSGSTWLSNVMGELGGHKIWREPKIGQL
                     FGEFYANTPKQKRDSANFIMGAPTRKAWISSIRNFVLVGARHTFPLLRPDQQLVIKEP
                     GGGVGAPLLMEALPESRMILLVRDPRDIVSSWLDAAGEGGWKRQNRRDSSNRESLAEK
                     RPDAVVRKLARRYLNNLQMARQA"
     gene            913..1845
                     /locus_tag="VHM69_07630"
     CDS             913..1845
                     /locus_tag="VHM69_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX2740303.1"
                     /translation="MARTHYYPDDRVHHVWDNEQEPVLTVEPGDTVVYNTREVSDGQI
                     TPDSDAGALDNLDWDRLYPLAGPVAMANAVPGDILAVEVLDIHTKGWGWTAVIPGFGL
                     LPEDFPDAYLKIFDLTNGDYTLFKDDIAIPIEPFFGTMGVSPAGPEKPIIMPPGPFGG
                     NMDTRHLTKGSILYLPVQHEGALFSCGDAHAAQGDGEVCVTGIESPMYAALRFDLIKG
                     RSIPAPQFQTPGPLTPVVEEAGWYATMGVNGDLMQAARDSLRAMVEHISTTYGLEPVE
                     AYVLASLVVDLKISEVVDQPNWIVSSYLPLAIFG"
     gene            1930..2262
                     /locus_tag="VHM69_07635"
     CDS             1930..2262
                     /locus_tag="VHM69_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX2740304.1"
                     /translation="MVTISKDNGVVTLINVFTVAPEDQQRLVDVLVDATQRVMNEQPG
                     FVSASIHRSFDGRRVTNYAQWRSREAFEAMLRNQEAAEHMGKAARIAERFEPHLYEVS
                     FVDENSRA"
BASE COUNT          481 a          752 c          684 g          420 t
ORIGIN      
        1 gcttgcctgg ccatctgcag gttattcaga taccttctgg ccagtttcct gacgaccgcg
       61 tcaggccgct tctccgcaag tgactcgcgg ttcgaggagt cgcgacggtt ctgcctcttc
      121 catccaccct cgcccgctgc gtcaagccat gaagacacta tgtccctcgg gtcccgaacg
      181 agcaggatca tccggctctc cggcagagct tccatcagca gaggcgcacc gacgccgcca
      241 ccgggctcct tgataaccag ctgttggtcc gggcgaagca gggggaaagt gtgtcgtgcg
      301 cccaccagca cgaagttcct aatagaggag atccaggcct tcctcgtcgg ggcgcccatg
      361 atgaagttcg cggagtccct cttctgtttc ggggtgttgg cgtagaactc gccgaagagc
      421 tgtccgatct tcggttccct ccagatcttg tgcccaccga gctcccccat cacgttgctc
      481 agccacgtgc tcccgcttct gcctacgcag aatatccagg tgatgttggc gggatcgagg
      541 cccaacgctg cagtgtcagc gacgccagat ctcccgcgta ccgaggacac gaaacgggtt
      601 atcccaaggg tgatgcctga cagcctgcgc ttcatggcgg gaagcgccgg ataccggacc
      661 gcggctcgcc gccacgccca cactttcctt tgcctcatcc aaccgccgaa agacttcttc
      721 atacgctcca aactatcgca accaatagga aagcagccgc atcccgtagc gtttctccct
      781 ggcaggttac acgaagatac ccggcgggaa aaggtcgcag acagcgcccc ttgagacact
      841 aaaccggtgg acggaagcgt gcagaggtgc gaaagtaact atcgggaaag gacgaacagg
      901 aggtcaagcg gaatggccag gacgcactac tacccggatg acagggtcca ccacgtctgg
      961 gacaacgagc aggagcccgt gctcacggtg gagcccggcg acacggtcgt ctacaacacc
     1021 cgggaggtct ctgacggcca gatcacaccc gactcagacg ccggtgccct ggacaacctc
     1081 gactgggaca ggctctaccc gctcgccgga cccgtcgcga tggcgaacgc cgtgcccggc
     1141 gacattcttg cggtcgaggt acttgacatc cacaccaaag gctggggctg gaccgcggtg
     1201 atccccggct tcgggctgct tccggaggac ttccccgacg cctacctcaa gatcttcgac
     1261 ctgaccaacg gcgactacac cctcttcaag gacgacatcg cgatccccat agagcccttc
     1321 ttcggcacga tgggcgtgag cccggccggt cccgagaagc ccatcatcat gcccccagga
     1381 cccttcggcg gcaacatgga cacccgccac ctcaccaagg gctcgattct ttaccttccc
     1441 gtgcagcacg agggcgcgct cttctcctgc ggagacgcac acgcggccca gggcgacggc
     1501 gaggtctgcg taacgggcat cgagagcccc atgtacgccg cgttgcgctt cgacctgatc
     1561 aagggcagga gcatcccagc cccccagttt cagacccccg ggcccttgac tcccgtggtc
     1621 gaggaggccg gctggtacgc cacgatgggt gtgaacggag acctgatgca ggcggccagg
     1681 gactccttac gcgccatggt cgagcacatc agcacgacct acgggctgga gcccgtcgag
     1741 gcttacgtgc tcgcgagcct cgtcgtagac ctcaagatct ccgaggtcgt ggatcagccg
     1801 aactggatcg tctcctcgta cctgccgctc gcgatcttcg ggtagcggcg gctccaggtg
     1861 cggggatgtt cttgtattag atacgagcta tcctagacaa gtcgcgcagc agcaggaggt
     1921 agacagcgca tggtcacgat ctcgaaggac aacggtgtag tgaccctcat caacgttttc
     1981 accgtcgcgc cagaggatca gcagcgtttg gtggatgtgc tggttgatgc cacccagaga
     2041 gttatgaatg aacaacccgg cttcgtctca gccagtatcc acaggagctt tgatggtagg
     2101 cgagttacca actacgcgca atggcgtagt cgagaggcgt tcgaggcaat gcttcggaac
     2161 caagaagcgg ccgaacacat ggggaaggcg gccagaatag ccgagaggtt cgagccgcac
     2221 ctctacgaag tctcattcgt agacgagaac tcaagggcat aggacgggaa aagagtcctc
     2281 tgaccgtccg gtcctgaata actttcaccg aacgccgagt tgccttgaga gctcctc
//
LOCUS       DATAIR010000315         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20085, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000315 DATAIR010000000
VERSION     DATAIR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20085"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1429
                     /locus_tag="VHM69_07640"
     CDS             158..1429
                     /locus_tag="VHM69_07640"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX2740305.1"
                     /translation="MWKPAPDRLDPIELLQGQEKTRQPDLARHRYARMMPSPFTFMRG
                     AAIIMANDLGGTPHTGIQAQLCGDCHLSNFGLYASPERTLLFDVNDFDETLPGPWEWD
                     LKRLATSMVVAGRENGFGDSGCRRAARAAATSYRKRMAEYSEMGELETWYSRVRAEEI
                     FDLISDTKSRKRVKKALTKARGRDSLQALSKLTRVVDGRRIFADDPPFLVRVVEDELR
                     EQIDEILQMYRRTLQDDRLHLLERYRFVDVARKVVGVGSVGTRAFVVLLEGRDEDDPL
                     ILQVKEAEASVLEGYLPQSTYENQGQRVVAGQRLMQAASDIFLGWIRGPGGRDFYWRQ
                     LRDMKGSAKIERMSPDELAIYANFCGWALARAHARSGDRVQISAYLGKSERFDGAIVD
                     FAVSYADQIERDHAALCAAVKSGKIAGDVGA"
     gene            1791..2387
                     /locus_tag="VHM69_07645"
     CDS             1791..2387
                     /locus_tag="VHM69_07645"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2740306.1"
                     /translation="MDEIFSLIEHYGYLFVFFGVMLGCSGIPFPGATILLAAGILVQQ
                     GHLELGYAIAFGILGAILGSQIGYWAGQRGGRSFVLKWGRYVKITPERLERVEQFFVR
                     YGGKAVFASRFVSVFRMLGAPVAGISRMRGSTFFLYSALGGMVWTTVVVLLGYFFGQS
                     WAGAQHWTGRGPLLLALLLAVALSLYVAYRWVLRYRRS"
BASE COUNT          518 a          809 c          826 g          534 t
ORIGIN      
        1 gagggcgatg aaagcccatc gagacccaca tggcgcagcg gacacgagag cgcctatcgt
       61 ggcggcagat cctcggggaa atactgcctc gagttatcag cagcggcgcg agcgtgggcg
      121 cgccgcgcgc cagagaacgc ctcggagcag ccacgcggtg tggaagccgg cgcctgatcg
      181 gctggatccg atcgaactcc tgcaagggca ggagaagaca cggcaacccg acctggcgcg
      241 acaccgctac gcgcgtatga tgccctctcc gttcacgttc atgcgcgggg cagcaatcat
      301 tatggccaac gacctggggg gaacgcccca taccggtatc caggctcagc tgtgtggcga
      361 ctgccacctc tcgaacttcg ggttgtatgc ttctccggag cgcactctac tgttcgacgt
      421 caacgatttc gacgagacat tgccggggcc atgggagtgg gacctaaaac gcctcgccac
      481 gagcatggtc gtggcaggca gggagaatgg tttcggcgat tccggttgcc gaagagcggc
      541 gcgcgcagcc gcgacttcgt atcggaagcg aatggccgag tattcggaga tgggagagct
      601 cgagacctgg tactcgcgcg tcagggcgga agagattttc gacctgatct ccgacacgaa
      661 gtccaggaaa agagtgaaaa aggccctgac gaaggcacgc ggccgcgaca gcctgcaagc
      721 tctctcgaag ttgaccaggg tcgtcgatgg tcgccggata ttcgccgacg atccaccttt
      781 cttggtccgc gtcgtggagg acgaactgag ggaacagatc gacgagatcc tgcagatgta
      841 cagacgcacc ctgcaagacg accgtcttca cctgcttgag aggtaccgat tcgtggacgt
      901 cgcgcgtaag gtcgtggggg ttggcagcgt gggcaccagg gctttcgtcg tattgctcga
      961 gggccgcgac gaagatgacc cgcttatcct ccaggtcaag gaggcggaag cgtcggtatt
     1021 ggaaggctat cttcctcaga gcacctacga gaaccaggga cagcgcgtcg tcgcaggcca
     1081 gcggctcatg caggcagcga gcgacatctt cctcggctgg atccgaggac caggcggccg
     1141 cgacttctac tggcgtcaac tgcgagacat gaaaggctcg gcgaagatag agcgcatgtc
     1201 tcccgacgag ctggcaatct atgcaaactt ctgcggctgg gcgctggcac gcgcgcacgc
     1261 ccgctccggc gaccgcgtgc agatctccgc ctacctcggc aagagcgagc gcttcgatgg
     1321 agccatcgtc gacttcgcgg tgtcttacgc ggaccagatc gaacgcgacc atgctgctct
     1381 gtgcgccgcc gtgaagtcgg gtaagatcgc aggagatgta ggcgcttgag aagttcatca
     1441 gaccctttga gccaaagtgg ggcggacgac cttatcagag ggcggagccg cctcctcaac
     1501 gctgcgaaac gctcggagat ggtacgaagg tatacgagaa ggacatagca tatctttccg
     1561 cactcgcaga cgctcgtgcc cgtcgaaaat caacccttct gaggtccctg atccacatgc
     1621 ctgcccgtgg cacagaggtc attcttgtct cgccactatg actcggcaga agccggcaac
     1681 ccatagcggc gcctcgtcac ctgatcgccc caaaacaaag gtccaattgg ggatctgctc
     1741 atgtcccggc ggtatcccgg agacggctgc cccctctata atctgctgca atggatgaga
     1801 tcttctccct gatcgagcac tacggttacc tgttcgtgtt tttcggcgtg atgctagggt
     1861 gttcgggcat cccattccct ggcgcgacca tcctcctggc cgccggcatt ctggttcagc
     1921 agggccacct ggaactcgga tacgccatag catttggcat ccttggcgct atcctcggca
     1981 gccagatagg ttactgggca ggccagcgag ggggacgatc gttcgtcttg aaatgggggc
     2041 gctatgtgaa gatcacccca gagcgcctgg agcgagtcga acagttcttc gtacgctacg
     2101 gcggcaaagc cgtcttcgcg tcccgcttcg tctcggtctt ccgcatgctc ggagcgccgg
     2161 ttgcaggtat aagccgcatg cgcggaagca cgttcttcct ctacagcgct ctcggcggaa
     2221 tggtgtggac tacggtagtt gtcttgttgg ggtacttctt cggacagagt tgggccgggg
     2281 cgcaacattg gacgggacgg ggccccctcc tgctggctct gctgctcgct gtggcgctaa
     2341 gcctttatgt tgcctatcgg tgggtgcttc gctacaggag gtcataggtc ttgcgcttct
     2401 tcgacggtgc cggtcatcca tgttcgcgtt ttcgcaacgg ctacgaacgc ggagattgcg
     2461 agccagaaga tccgtgagcc gtggatgccg tacgtcgaac gcctatattc ctgtcttata
     2521 tgcccacctt cgtccccgcg catcaggcca tattattctt acccgacgca ccgatgtaaa
     2581 tgggctgcgc gcgctccggg ccttctactt cgtccactct accttcggca cccccgattg
     2641 gtcgaactct ccgaccctct cacgcccgag ctcttccagc tctctga
//
LOCUS       DATAIR010000316         9257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_112570, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000316 DATAIR010000000
VERSION     DATAIR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112570"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VHM69_07650"
     CDS             <1..1309
                     /locus_tag="VHM69_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX2740307.1"
                     /translation="EVAVVVATIAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGR
                     DGERAECVVLYRGADLGRRKRLVTLGAAGEREVSSFFRALAGVENGGRVNVPPSSLSA
                     LGGVDAESAGIVMGSLEESGLISRGYDLWAEVEVRALEEEPRGLREEVAAVHAALPGS
                     GSVGLPELARRAGLRPAVVQGALYRMMVDGIVEIIPRGSLVDVRLKTSSLDEASRRGI
                     AGRLKNRATTAYAQIRDVESFANLKTCRREHLLRHFGDKEEVAPCAGCDVCLGETEEL
                     VRKAGSRADVPDGEPLVPEMSEKGPADAALFERLRSWRGEQARKQRVPAYVVLHNSHL
                     EEICIRKPRTIHELGAIKGVGLRRAARYGEELLALVRGEEAEDATPVQEDNGYRGHLE
                     AASLLLRSGRGADAVPELTRALEIGGEEARREVDELLKSVAGE"
     gene            1332..1853
                     /locus_tag="VHM69_07655"
     CDS             1332..1853
                     /locus_tag="VHM69_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX2740308.1"
                     /translation="MRLNGKKVAVLVAEGFEDLEYWVTVMRLQEEGVEVVSVGPDTGP
                     VTGKNALQAQADTTADNVDASELDGVVIPGGWAPDKLRRYPEITDLVRTVHERNKPVG
                     IICHGGLVAISAGIVEGVSATGSLGIKDDLENAGATWVDKAAFREGNLVWGRVVPDIP
                     DFCRELVAALAEQ"
     gene            complement(2417..2905)
                     /locus_tag="VHM69_07660"
     CDS             complement(2417..2905)
                     /locus_tag="VHM69_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740309.1"
                     /translation="MGRQTRAHQDVDVAILRADQKQLHEAFGGWELYYATTDHRLVPY
                     RSDRWLELPLHGVWVRPASDAPWLCEFLLNEHEGQQWVYRRNPAVRKPLAEVGVIALG
                     GVPILVPEIVLLYKAHELTEKDEADFRTALPHLTASRKAWLLGALDETRPNHPWARRL
                     RG"
     gene            3333..4313
                     /locus_tag="VHM69_07665"
     CDS             3333..4313
                     /locus_tag="VHM69_07665"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX2740310.1"
                     /translation="MIGERIRHGTRLRHRCMFALVIALLGMLALSTVAIAQAVRVAED
                     VREYEVPASPPEGVVYDQKTQAFYGGSVSDGLIYKGTLDDPTAEVFLPPGSDGRNEAI
                     GVNVDREGRLYIAGGSSGTLYVYDIETKELLARFETGEGGFINDVAISKYGDVYFTDS
                     IRPFIYRVSEEQVEAGGGTPEAIPLTPEVDYQGFPNANGIRTTPNGKYIIFADTNDGK
                     LYRMVPAEDPTEREISEIGVRGQTDNPDGLELRGHTLYAVNNQDELIVEVRLSGDYLG
                     GRVVGNTTTPEFHTPTTASLAGGRLLVANAEFFDQSESGPPFYVVSIPRP"
     gene            4546..4956
                     /locus_tag="VHM69_07670"
     CDS             4546..4956
                     /locus_tag="VHM69_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740311.1"
                     /translation="MSAKKSKGFTDEERAAMKDRAKELKAEARESDVLAKIAEMSEPD
                     RALGKRLHEIIKVSAPALSPKLWYGMPAYARDDKVVCFFQSAQKFNSRYATFSFSDKA
                     NLDEGAMWPTSFALKELTATEEAMISALVKRAVS"
     gene            5159..5407
                     /locus_tag="VHM69_07675"
     CDS             5159..5407
                     /locus_tag="VHM69_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740312.1"
                     /translation="MREDLTPEQRRSLEGLVREKGERCGLCGNSDLRCGKDAAEVGGG
                     YSVQLRCTNGKAGVHDGGIGLIWGYTITSDEARRVGLG"
     gene            5580..5774
                     /locus_tag="VHM69_07680"
     CDS             5580..5774
                     /locus_tag="VHM69_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740313.1"
                     /translation="MLNRDSESVENAFATHAGGVGELLLSLTAQEAQVILARVQERAA
                     IEAEDFWGQADEACSVDPIG"
     gene            5941..6471
                     /locus_tag="VHM69_07685"
     CDS             5941..6471
                     /locus_tag="VHM69_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2740314.1"
                     /translation="MRRVRYVVAMSLDGYIAGPNGEADWIVMDPEIDFRALFEQFDTF
                     LLGRRTFEGMGGARGGRQSGVQTIVFSRTLRQQDYPNVTIVSEKPKQVLANLRSKPGN
                     DIWLFGGGSLFRSLLEARLVDAVEVSVVPVLLGGGIPMLPPDPLSERFKLKLASSRAL
                     KSGIVSLEYAVEYEPA"
     gene            6563..6928
                     /locus_tag="VHM69_07690"
     CDS             6563..6928
                     /locus_tag="VHM69_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740315.1"
                     /translation="MTHKYEWTYTAALDHHKHSRKKVKEWVRSVLEASNWFARAGIRM
                     RYVDNPIQAKTIITYARKSEVCGGIGCTRTFPGNSRKDQIFSIREDLTFVLRCASARD
                     VPQLPHGGGPAWRRALPRP"
     gene            7283..7657
                     /locus_tag="VHM69_07695"
     CDS             7283..7657
                     /locus_tag="VHM69_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740316.1"
                     /translation="MTERRAAEEALGHVAKPKPAKSLPNPDWDSGNVPMTPLPPGSPS
                     QWHTMNHGLGSTDLLVDVKLGGTNAQGDVVWSLSTETLFALTGLNSLTYRWNLDEEVW
                     ERYKSQLGITGGPRLRVLVWRL"
     gene            8380..8958
                     /locus_tag="VHM69_07700"
     CDS             8380..8958
                     /locus_tag="VHM69_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740317.1"
                     /translation="MRTCSRSTNPQIANVPDITEVEAEAYAEPLGQAMLKHGCTSRRR
                     AAHYLAQAGYESGCFNWLEEFGGPDAWYAPYYGRGAIMLTHRENYKALGDLMGVNIVD
                     DPGLIGCKNNNPVEWPRDTARCMEVGPAFFRWRDLWTLADRGDAGFEPIMLRVLGAIN
                     HPSYNDRWALYRAMINKLPRPLFVEAGYCRRP"
BASE COUNT         1965 a         2574 c         3052 g         1666 t
ORIGIN      
        1 cgaggtcgcc gtcgtcgtcg cgaccatcgc cttcgggatg ggcgtggaca agccgaacgt
       61 gcgcttcgtt atccattcga gcgtcccggg aagcctcccc gcctacatac aggagtcggg
      121 cagggcggga agggacgggg aaagagccga gtgcgtcgtg ttgtatcgcg gcgccgacct
      181 cgggcggcgc aagcgtctgg tcacgctcgg cgccgccggc gagcgcgaag tgtcctcctt
      241 cttccgggcg ctcgccgggg tggagaacgg ggggagggtc aacgtgccgc catcttcgct
      301 ctccgcgctc ggtggcgtgg atgccgagtc cgcgggtatc gtcatgggga gcctcgagga
      361 gtcgggtttg atctcccgcg gctacgacct ctgggcagag gtggaggtgc gcgctctgga
      421 agaggagcca aggggacttc gcgaggaggt ggccgccgtg cacgccgcgc ttcccggctc
      481 ggggtccgtc gggcttcccg agctggcccg cagggcagga ctgcgtccgg cagtggtcca
      541 gggcgcgctg tacaggatga tggtcgacgg tatagtggag atcatccccc gcggctcgct
      601 ggtcgacgtg aggctcaaga cctcgtcgct ggacgaggcg agccgccgcg gcatcgccgg
      661 caggctgaag aacagggcca ccacggccta cgcccagatc cgggacgtag agtcgttcgc
      721 caacctgaag acctgccgcc gcgagcacct cctcaggcat ttcggcgaca aggaggaggt
      781 cgccccgtgc gcaggttgtg acgtctgcct cggcgagacg gaagagctcg tcaggaaagc
      841 cgggtcgcgg gcggacgtgc cggatggaga gcccctggtc cccgagatgt cggagaaggg
      901 accggcggat gcggcgctct tcgagcggct gaggagctgg agaggagagc aggccaggaa
      961 gcagcgggtc cccgcatacg tggttctcca caactcgcat ctcgaggaga tctgcatccg
     1021 caaaccacgt accatccacg agctcggcgc cataaagggt gtcggcctcc gccgcgctgc
     1081 ccgctacggg gaagagttgc tcgccctggt ccgcggggag gaggccgagg atgccactcc
     1141 cgtccaggag gataacggat atcgagggca cctcgaggcc gcatccctac tcctcaggtc
     1201 cgggcggggg gctgacgctg ttcccgagct cacccgcgcc cttgagatcg gtggtgaaga
     1261 ggcgcgacgt gaggtagacg aacttctaaa gagcgttgcc ggagagtaag agatctacga
     1321 gaggaggccc tatgaggctg aatgggaaga aggttgcggt cctggtcgcg gaggggttcg
     1381 aggaccttga gtactgggta acggtaatgc gtctgcagga ggagggggtc gaggtcgtct
     1441 ccgtcgggcc tgacaccggg ccggtaaccg gtaagaacgc cctgcaggcg caggcagaca
     1501 cgaccgcaga taacgttgac gcctccgagt tggacggcgt cgtgatccct ggtggctggg
     1561 ccccggacaa gctgcggcgt tatcctgaga taacagacct ggtcaggacc gtccacgaga
     1621 ggaacaagcc tgtcggcatc atctgccacg gtgggctcgt cgcgatctct gccggcatag
     1681 tcgaaggggt cagcgcgacg gggtcgctgg ggatcaaaga cgacctcgag aacgcagggg
     1741 ccacctgggt ggataaagca gccttccgcg aaggcaacct ggtctggggc cgcgtagtcc
     1801 cagatatccc tgacttctgc cgcgaactcg tcgccgcgct cgccgaacag taggagagtt
     1861 ctgacacacc aacggataca ccaatgcggg ggttaggtga atagaggccc aggaaggggc
     1921 cagagtgccg cttgacgcgc ctcagtgttc tcgggtggaa cgctggcgtg cgtgtgatcc
     1981 aaatcgcgag cggcgaaagc ctttaggttg atggcgtcgc tcacagtttt taccttgagt
     2041 cttggatggg gtctgtatgt cgggaagaaa agtgggtgag tcggatgaaa ctcataacgg
     2101 cgcagcagaa gatcgagcaa ttggtgagga agtagatctc ttcgccaaac atattcataa
     2161 gctggcagaa ttggtgacac tacagagatc gacacggtag gcacgtgcct acaaagctgc
     2221 acctcctcgt tggccgcgca accgctgcta tcagccgtac catgaagggc agcgccgttg
     2281 tcgtaatcgg cgttgacgga ccaagtcggt cgcgacaaca gcttccactg gcgctccgga
     2341 gaaggtctat tctgtgaact tcgcctgtac ggcgttctaa gtagtttggc cgtagtgctc
     2401 agtcggtcaa cagcttctag cctcgcagtc gccttgccca gggatgattc ggcctcgtct
     2461 catcgagcgc ccccaacagc caggccttcc gactcgctgt caagtgggga agggcggttc
     2521 ggaagtcggc ttcgtccttc tcagtaagtt cgtgggcctt gtagagtaag acgatctccg
     2581 gcactagaat cggcacccca cctaaagcta tcacaccaac ctcggctagc ggcttccgca
     2641 ctgccggatt tcgccgatac acccactgct ggccctcgtg ctcgtttagg aggaactcgc
     2701 aaagccaggg agcgtcggac gccgggcgaa cccacacccc gtgcagtggc aactcgagcc
     2761 agcggtcgga gcggtaaggc acaagccggt gatcggtcgt ggcgtagtaa agttcccacc
     2821 caccgaacgc ctcatgaagc tgcttctgat cggccctgag gatcgcgacg tcgacatcct
     2881 ggtgagcacg cgtttggcgc cccatctaga gatccagcgc cctgcctccc gccacccacc
     2941 agggcgcatg aaagccgcgc atgaggctcg aaagccggga aggatcgaag tcctcccacc
     3001 tccaaaggtc accgtagtcg aagccgatca caacaagcat tagaacgcaa agcgcagcct
     3061 cctgccagga ggctcattca cccgagtgcg tggaaaggcc ctttctccga aggttactaa
     3121 gaacggagca agcttgacgt tgaggcgtaa ttccgttgtt gtgatcagcg ttggccgaca
     3181 cggagtcagt cgtgacaaca gcgtcgtgcg gagcatgtcg agaaccgagg gtgttcgccc
     3241 tgtgctagga tgccatggcg taaagggaat agtgggaaga tcgcctcagg ttacggcagc
     3301 tgaggcgggc cgaagaatag gaaggtgagc ccatgatcgg cgagcggatc aggcacggga
     3361 cgcgacttcg gcaccgctgc atgttcgcgt tggtaatagc gctgctcggt atgctcgcat
     3421 tgagcaccgt ggccatcgcc caggcggtac gcgtcgccga agacgtacgc gaatacgagg
     3481 tcccggcgag cccgcctgag ggcgtcgttt acgaccagaa gacgcaagct ttctacggcg
     3541 gttctgtgtc cgatggcctc atttacaaag gtactctgga cgatcccacc gcggaggtct
     3601 tcttaccgcc cgggagcgac ggacgcaatg aggccatcgg cgtgaacgtc gacagggagg
     3661 ggcgcctcta catcgcgggc ggcagcagcg gaaccctcta cgtttacgat atcgagacca
     3721 aagagctgct cgcccgtttt gaaacggggg agggtgggtt catcaacgac gtcgccataa
     3781 gtaagtacgg cgacgtttac ttcaccgact ccatccgccc ctttatctac cgggtcagcg
     3841 aggaacaggt tgaggccggc ggaggaaccc ccgaagccat cccgctcacg cctgaggtag
     3901 attaccaggg cttccccaac gccaacggca tcaggacaac ccccaacgga aagtacatca
     3961 ttttcgccga caccaacgac ggcaagctct accgtatggt gccggccgaa gatccgacgg
     4021 agagggagat cagcgagata ggggtgcgcg gccagacgga caaccccgac gggctcgagc
     4081 tcagaggaca caccctctac gcggtgaaca accaggacga gctgatcgtc gaggtacggc
     4141 tttctggtga ctacctcggg ggccgggtgg ttggcaacac gaccaccccc gagtttcaca
     4201 cgccaacgac ggcctcgctg gccggtggtc gcctgctggt ggcgaacgca gagtttttcg
     4261 accaatcgga gtccggaccg cccttctacg tcgtgagcat cccgcgacct tagcgcttcg
     4321 tcggttctag accgcgggag gcggatgtgg tcggcgcgag cagtcaggtg ttgtggcgac
     4381 cgtgtagctt ggcgcagagt aggttgcgac aacgcttcct gaattgcttc ggttgcgcag
     4441 agttttaagg ttaatgctgc gaacttcgcc tgtacggagt atctttgagg gatggaccgc
     4501 agacccagaa agtaggacat gggagagcct gcacaggagg gaacgatgag cgcaaagaag
     4561 tccaagggat tcacggacga ggaacgagcc gcgatgaagg accgcgccaa agagcttaag
     4621 gcggaagcgc gtgagagcga cgtgctcgcg aagatcgccg agatgtcgga accggatcgt
     4681 gccttgggca agcggctcca tgagatcatc aaggtaagcg cgccagccct ctcgccgaaa
     4741 ctctggtacg ggatgcccgc gtatgccagg gacgacaagg tcgtctgctt cttccaaagc
     4801 gcgcagaagt tcaactcgag gtacgcgacg ttcagcttca gcgacaaggc gaacctcgac
     4861 gaaggcgcca tgtggccgac ctccttcgcg ctgaaggagt tgactgccac cgaagaggca
     4921 atgatcagtg cgctcgtgaa gagagcggtg agctgagccc atctcctgag caccgctccc
     4981 tgcccagtca ctacgatcgc gaaggagaac cagacgtact gctcgaaggt gctcagatcg
     5041 agccagggcg acgcagactg taaggcggcc ccgccgccga aggtcctcag gagggcgtac
     5101 agtggccctg gtggcgtaga atgttcggag gatagcgcga agaagggagg gagtcagcat
     5161 gcgagaagac ctcaccccag agcaacgcag aagcctcgag gggctggtgc gcgagaaggg
     5221 cgagcgttgc ggactctgcg ggaatagcga tctgcggtgc ggcaaagatg ccgccgaggt
     5281 agggggcggc tacagcgtcc agctgcggtg taccaacggc aaggccgggg tccacgacgg
     5341 agggatcggc ctgatatggg gctacacgat cacctcagat gaggcgaggc gggttggcct
     5401 cggctaaggc atcttgggat gaccttcgcc tgtacggagt tctctgaagt tcgctacccc
     5461 ttgcaacctc taagcaacaa caagaatacg ccacctggca gatgcgctgg ggtgtgcctt
     5521 gtgcctatag ttgcttcagg gccgggagat gcccgaccaa gaaggagagg agcgagcaga
     5581 tgctcaaccg ggactcagag tcggtagaga atgcgttcgc gacccacgcg ggcggtgtag
     5641 gggagttgct actttcgctt accgcccagg aggcgcaggt tatcctggca cgcgtacagg
     5701 agcgtgcagc catagaagcg gaggacttct gggggcaggc cgacgaggcc tgctctgtgg
     5761 atcccatcgg gtgagacact aagagtgagg catttatcgt aggaacaagg gctttggcct
     5821 gactattcac ccgggtgagt ggaggtcaaa ttctgccaac ttcgcatgta cggggttctc
     5881 ggatttttgg ggtttgatgt agactcccgt tagcttggcg gtgaggaagg gaattctccg
     5941 atgcgacggg tccgctacgt ggttgcgatg agcttggacg gctacatcgc gggtcccaat
     6001 ggtgaagcgg attggatcgt catggacccg gagatcgact tccgagcact cttcgaacag
     6061 ttcgacacgt ttctcttggg acgccggacg ttcgagggca tgggaggagc aaggggcggc
     6121 aggcagtcag gagtgcagac gattgtcttc tcgcggacgc tgcgccaaca ggactatccg
     6181 aatgtcacca tcgtttccga gaagccaaaa caagtgctgg ccaacctccg gtcgaagccg
     6241 ggcaatgaca tctggctctt tggcggcggt tctctcttcc gcagcctcct ggaagccagg
     6301 ctagtggacg ctgtcgaagt ctctgtggtt cccgttctgc taggcggggg cattccaatg
     6361 ttgccacctg atcctttgtc ggagcggttc aagttgaagt tggctagcag tagggcgctc
     6421 aagtcaggga tcgtgtcgct cgagtacgcc gtcgagtacg aaccagccta gcttcattct
     6481 gaccagtact ctttggactt acgacacacg gaccaaggcg acagcggacc gaaaggagtt
     6541 cctcacctct gatggcgtca ccatgaccca caagtatgag tggacctaca cggcggccct
     6601 ggatcaccac aagcatagcc gcaagaaggt caaggaatgg gtcaggagcg tacttgaggc
     6661 aagcaactgg ttcgcccgcg ccggcatccg catgcgctac gtggacaacc cgatacaggc
     6721 aaagaccatt atcacgtacg cccgcaagag cgaggtctgt ggcggcatcg gttgtacgcg
     6781 aaccttccct ggcaatagcc gcaaggacca gatctttagc atccgagagg atctgacctt
     6841 cgtccttcgc tgtgcttccg cacgagatgt gccacagttg cctcacggcg gtggaccagc
     6901 atggcggcgt gcgttgccgc gaccctaact acaacggctt gatgggtaat ggcaaaggca
     6961 tcatgacgga gaacgacgtg gcgcgtataa gggcttggtt gagaggcgag ggccgtatct
     7021 gccaggcggc cacttgagca ataaagcacc gttgtgccct gcgatgcttg atctgcctta
     7081 gcctcttgtc tctagcgcga taatgtcggt aagcgagacc cagatggtac cttcgtcccc
     7141 accatggatt gttcagccgg tcgcaccaaa atctagtctg gactcaacgc cgattcatcg
     7201 cccggcgcta gtgtgggcgg tggtcagcga agctggcggc gcagaggatt agcgacgctt
     7261 ccgtaaggaa ggaggagccg acatgaccga gcggagggct gccgaggagg ccctggggca
     7321 tgtggcaaaa cccaagcccg ccaagagtct gccgaatccg gattgggatt cgggcaacgt
     7381 acccatgacc cccctcccac cgggcagccc ctcccagtgg cacaccatga accacggcct
     7441 gggctcgacg gaccttctgg tcgacgtcaa gttagggggc acaaacgccc agggtgacgt
     7501 ggtatggagc ctgtcgaccg agacgctctt cgcgctcacc ggtctgaata gcctgacgta
     7561 caggtggaac ctggacgaag aggtgtggga gcgttacaag tcgcagctgg gcatcacggg
     7621 agggccgagg ttgcgggtat tggtctggcg cttgtaagtg tgggtgcgtc ccgatagagc
     7681 acaaaatggc ggaggcagca gggaggcacg ccgtcgagtg gcgtgctatg aagggcagca
     7741 ccgttgtcct gatcggcgtc gggcggcgta gccgaagaca gtacaagggc tcgatgtagc
     7801 ctggcgcaag ggtgtcgctg cgctctgaaa cagcttgact ctcttggtcc tgaggcgact
     7861 cggtggagcg atgtaaccct atcggtatct gtccccacga tgctcggata gagcaatacg
     7921 gcggtgcata ctccgacagc cgggagccat gtcttcgcat caacggcggt tgaggatgtg
     7981 aacgctgccg gcctcacggg cgattccggc gcagcaataa cgtcttggcg ggattcatca
     8041 agaattcatt cccgattcat cgcctcctga gagcctccac ctgagggacg agattactag
     8101 ctgagtagga agcgaatctc gctgtggttg gccgaaagca taggagtggt gagagatctc
     8161 ttgtcgcaga ccatgagagg aggtgaacat gagagatggc atcgagcatg gtgtgagaag
     8221 caagcccaag agagggagtc agcaaatcag ggacgccccg gcctgaccga gagtgacaca
     8281 caccgagcag atcgacatgt acaaaggagc gacgtggtgg aggagatgat cgtggtaggg
     8341 atggaggcga actaatggct ctcaaggtga acttcgacca tgaggacctg ctcaaggtct
     8401 accaacccgc agatagcgaa cgttccggac attacggaag tagaggcaga agcctacgcc
     8461 gagcctctag ggcaggcgat gctaaagcat ggctgcacct cgaggagaag ggcggctcat
     8521 tatctggcgc aggccgggta tgagtcagga tgtttcaact ggctcgagga gttcgggggg
     8581 cccgatgcct ggtacgctcc gtattacgga cggggagcca tcatgctcac ccaccgggag
     8641 aactacaagg cactcggcga cctgatgggc gtcaatatag ttgacgatcc cggtctaata
     8701 ggttgcaaga acaacaaccc agtggagtgg ccaagagata ccgcacgttg tatggaggta
     8761 ggtcctgcat tctttagatg gagggatctg tggaccttag cggaccgggg tgacgcgggc
     8821 ttcgagccga taatgctgag agtattgggt gccattaacc acccgagcta caacgatcga
     8881 tgggcgctgt atcgtgccat gatcaacaag ctccccaggc cgctgtttgt cgaggccggg
     8941 tattgcagac ggccttaaca gaggaggttc tcctggaaca cagtatcagc gtatgacctt
     9001 cgagagcgca aagggaaagt ctctccgggc tgaacagaac gaggctcggt gccgagcggg
     9061 tagaacggta cgagttccct ggattatcaa ggttcttccc ggctgccaac cttccgcatc
     9121 atcaccgaaa gctgggaaga accataggat cgggcagggg ccattctcgt agctcaattt
     9181 gcctagtaaa agatggctca catgggggac gcagaaccgt ctgcgcgtag ctatcatcag
     9241 gctatgaaaa agcctgg
//
LOCUS       DATAIR010000317        13097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_148018, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000317 DATAIR010000000
VERSION     DATAIR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148018"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1142)
                     /gene="murG"
                     /locus_tag="VHM69_07705"
     CDS             complement(57..1142)
                     /gene="murG"
                     /locus_tag="VHM69_07705"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX2740318.1"
                     /translation="MNEPERSNHTRPRILIAGGGTGGHAIPALCVAADLRERGALVEF
                     VGSQSGIESTLVPSAGFTLHTLPLTGLAGNPIARARAILLSLKAIRHCRRIIQRLAPR
                     AVLGVGGYASAPAVTAARTLGVPTFLHEQNSVPGRVNRLASRVTTETLVTFPEAARLL
                     RGAVWVGMPTRSEVFDASRKEAMRELGLEPPVVLIFGGSGGALRINLAAAEAFRGETS
                     YTVVQVSGRRDFARLSTDNPKHRILEYADDIWRYLAAADVVVSRAGAGSLFDIAAAGK
                     AAILIPFPYATGDHQLHNARYFVERGAAELMLDSEVTAEALRERVEDLLDDDERRETF
                     ARRMGDLATPGAAAEVAERLLRAGEKE"
     gene            complement(1042..2187)
                     /locus_tag="VHM69_07710"
     CDS             complement(1042..2187)
                     /locus_tag="VHM69_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX2740319.1"
                     /translation="MSSLRANLFLVALGLSLLGLVMVYSATYREVGTEYLFVRTGHMV
                     LGVAAFFAASRIRYTAWRRYAPALYVIVLVGLVLVLIPGIGNQVNGARRWLDLGPMSV
                     QPAEFAKLAAVLALSCAVARLPRGSKLPVKPVVAAGVLFALVLVEPDFGTSVVLLSGV
                     AGVLWASELRTKDLVLSGLAGLAAVIGMMLLAPYRRERFMTFLDPWAAADGSGYQVVQ
                     SMVAIKAGGFFGAGAGSGGQGPYVPELGTDMIFALIGEELGLIGMVVVICAFGFIALA
                     GLRIAMNAPSVMARCVAAGLTTMLTVQAIFNIGAAMVVLPLAGMTLPFVSYGGSSLLV
                     CFAAVGILYRISEDSERAREIKPHKATNPDRRRRHGGARYPRAVRGG"
     gene            complement(2184..3509)
                     /gene="murD"
                     /locus_tag="VHM69_07715"
     CDS             complement(2184..3509)
                     /gene="murD"
                     /locus_tag="VHM69_07715"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2740320.1"
                     /translation="MKTLVYGLGESGVAATRALVERGEEVIVADAGDDEHLRDVLAGL
                     DVSGFLSAGPEVLEGTDRVIASPGIRPSDPVLQAAEARGVPVVSEVGLGLDLLGDDAR
                     VVAVTGTNGKTTVVDMVHRMLDAADVRHAVAGNSWRALTGCVEEARNAGILVLEVSSF
                     QLHYLQSPGFEVAALLNVRPDHLNWHASFDEYVRDKLRIFDGQQAGDLALLNTEDRAG
                     SGSLKDLEAEALIVGDGDTSVNDGRLLLRGSHVADTAELHFAGLHNYQNALFAAAAAE
                     RLGAGLPEIREGLLGYKLKPHRMQVVAERGYVTYVDDSKATNPAAAAAALDSFDVPVV
                     LILGGSEKDTDFSELMPGLGACRAVICQGEAGPRIASYLEDEGWQDVVHRVPDLEGAV
                     AEAGSFARPGDVVLLSPGCASFDQFPGYAERGDAFTRFARELTGGAVGR"
     gene            complement(3514..4509)
                     /gene="mraY"
                     /locus_tag="VHM69_07720"
     CDS             complement(3514..4509)
                     /gene="mraY"
                     /locus_tag="VHM69_07720"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2740321.1"
                     /translation="MFSVVLAAAVAFLVTVMLGSKFIEFLQSRKFGQFVREEGPQTHL
                     IKAGTPTMGGIVMLTGLLGALVVVARLNVATLTTLLLVAVVAGIGLYDDWQKISRQRP
                     EGLSARYKFLLLTLAVVLADVLAVRYVGVTQTVLVPYFDRSLVLGPGVVGIALFSIFL
                     LLVIAGTTNAVNLTDGLDGLAAGAAAIALLAYTAIAFLERQYDVAVICGAMVGAIVGF
                     LWYNSHPADIFMGDTGSLAIGGVLAAAAVLTKTELLLPIVGGLFVVEAVSVMIQVFIF
                     KLTGRRVFKMAPIHHHFEFLGWDENKIVVRFWIAQAAFSSVGFFLYYIFLYNAVG"
     gene            complement(4502..5866)
                     /gene="murF"
                     /locus_tag="VHM69_07725"
     CDS             complement(4502..5866)
                     /gene="murF"
                     /locus_tag="VHM69_07725"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX2740322.1"
                     /translation="MRPASLSEVARAIGADLPATHGSALVGGAAIDSRKAREGDLFFA
                     LRGRSDGADFAPEAHLRGAVATVAARRLDVPTLVVGDPLKAMQRLARWTLLREDSPAV
                     VGVTGTVGKTTTKDALATILRHAGKRVSATAGNLNNEIGLPLTVLTSDPRTQVLVLEM
                     GATHAGDIEYLCSIAPPEIGVLTAISPVHLDSFGSIEALAAAKGELALALPDSGTLVA
                     PAGAPEAATGSGRKLKRITFARDADADLFASHVEDRDEGLHFVAHLLERSAEVYSPVF
                     GTHLVEPLLASVGGALALGLGLGEAARGLSRLRRTGLRGDVYRLKEDITVYDDTYNAS
                     PAAVAAVLRYGADQAARQGRRLIVVLGGMFELGACARAYHQEAGKLAGEVGVDVLVCV
                     GDEARWYAEAFPGKSLFYEDAETAAEALRDELRSGDYLIVKGSRGVGLDALTRKLRES
                     LALV"
     gene            complement(5863..7461)
                     /locus_tag="VHM69_07730"
     CDS             complement(5863..7461)
                     /locus_tag="VHM69_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2740323.1"
                     /translation="MVAAVFAVTGLLLGGRAVHISLTQDESYQAFADEQVGGEAQVAE
                     PTRGSIVSADGRELATSLEVARVVATPYQVEDPHATARKLDAVLGQATDRSLDELQAA
                     LAKRDASGRLTGYSVVATDVKPETAARVQALGIEGITIAPDTLRVYPDGSLASQLIGH
                     QGDFGVFGGVEARYDERLKSGDDVTLTLDTAVQQELQTALDAAVEKSRARSAVGVVMR
                     VDDGAIVALANTPAYNNNKFGNVSAEAQRDRVLTDPYEPGSTFKPITVASALEEGAVT
                     PESTFVVPDHMTVADRVINDSQPHETEIMTPADVLKQSSNVGATKVALELGGLKLYEY
                     MRAFGFGEPTGVDLWGEDPGSVPAYEDWSGVSIGNIPFGQGFTVTPLQLVAGYATLIN
                     GGLKVTPHVSEQENPETPGPRVISEETSAIVRGMLQGVVDDGSGHFAQISGYTVGGKT
                     GTSQKVDPKTGTYGDQYVASFIGFAPATDPEYVTLIAVDEPQTSYWGELVAVPAFHQV
                     MSFTLGYFNVPPDRRGFEAEEASQ"
     gene            complement(7658..8095)
                     /locus_tag="VHM69_07735"
     CDS             complement(7658..8095)
                     /locus_tag="VHM69_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740324.1"
                     /translation="MAASQRRFQATLPTEPAWPVRKHAPDRSPDRAHLRKAAARRRRF
                     AMLVVVPVLLMLGSVYLHTVGAGLDTRAAELGVKLDRAGDQGERLKVRVAELSGAGRI
                     RELAAERLGMHEPGSDELKVYANEAEDGTQDGASEEGGKPRQR"
     gene            complement(8095..9030)
                     /gene="rsmH"
                     /locus_tag="VHM69_07740"
     CDS             complement(8095..9030)
                     /gene="rsmH"
                     /locus_tag="VHM69_07740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX2740325.1"
                     /translation="MATGHRPVMLEEAVSALRLGPDDAVVDATFGGGGHSLRILDELG
                     PEGNLVGIDRDPEAIGRASGLLQDPRFEFVSGAFDEALWEMVEEGRRADGVLFDLGLS
                     SFQVDEPERGFSYVREGPLDMRMDPGSGVSAAEYLNTVEVGELARVLSEYGDVPRGEA
                     GRVAREVVRRRPLEVTTDLYEAVRAAVGWKEKGGNPAKRIFQAVRVRVNDELGGLGRA
                     LEASERLLTSGGRLVVITFHSGEDRLVKRFISEREGRCVCPPELPVCVCGARPVFRRD
                     AVQTPSMGEVEANPRSASARLRSAIRTAEPPSDGT"
     gene            complement(9030..9470)
                     /gene="mraZ"
                     /locus_tag="VHM69_07745"
     CDS             complement(9030..9470)
                     /gene="mraZ"
                     /locus_tag="VHM69_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX2740326.1"
                     /translation="MALLGEYEHTLDDKGRMTLPSRLRPYFEGGIVVTKGVDRCLFAF
                     PPEEWAAFKANIKANADLSARGRQLSRMFFSMAFEATLDRQGRVLIPTKLARYAGLQR
                     DVTITGVDDRLEIWDTEEWDRYVSGADDSFSEIVEEFAGRDLGG"
     gene            complement(9800..9876)
                     /locus_tag="VHM69_07750"
     tRNA            complement(9800..9876)
                     /locus_tag="VHM69_07750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9840..9842),aa:Ile,seq:cat)
     gene            complement(9907..9979)
                     /locus_tag="VHM69_07755"
     tRNA            complement(9907..9979)
                     /locus_tag="VHM69_07755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9944..9946),aa:Asn,seq:gtt)
     gene            complement(10020..11780)
                     /gene="dnaG"
                     /locus_tag="VHM69_07760"
     CDS             complement(10020..11780)
                     /gene="dnaG"
                     /locus_tag="VHM69_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX2740327.1"
                     /translation="MRISDRSIEEVREAANIVEVTSEFTALKRQGARFAGLCPYPDHQ
                     EKTPSFSVSPDRGFYYCFGCQRGGDAIKLVMDLKSFDFAEAVAYLAERSGVDVQFEGG
                     GDPGAARERTLRRREIHKALAAAAVYYHKYFLRSQTPEAEQARRYLRDRHLERSTIEE
                     FRIGYAPPRGSSGFLGVARKLNLDRSVLNAAGLLSSRGGERFAGRITFPISDRRGRIV
                     GFGARTLGDAQPKYLNSPETEIFNKRDLLYGFPQVSEAIRRERAALVVEGYTDVLMLY
                     QSGIKNAVATLGTATTPAHLRTLSGYADRIYVLFDPDAAGEKAIERAAATAADLKLDL
                     RVMRLPDDPADWLLEHPPEEFAEMLKGAVPVLEYSIRRIVARTRGADATERSKAVPEI
                     KSMIQEIKDPVLYREAVRLATESLGVNPEVLQTSEPATTRASSPRGSRQTDPLTEAGG
                     AVLALVLARPDLTAPLLENGIQTQALPEPFTLRTEDFRDDVYARIFNVLKEHGGEDLD
                     AVLSDERARPLMDQIGALASQGERLFPSESSVREAWLRLGILSRERRKRETPDYDEKE
                     SLQSEIHQLKVALRSVSAGA"
     gene            11814..>13097
                     /locus_tag="VHM69_07765"
     CDS             11814..>13097
                     /locus_tag="VHM69_07765"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX2740328.1"
                     /translation="MRSVVVQNNGNGTLPDVDVRDRSTPVPESGFADLPPGIGFESKD
                     EPLRRDINLLGRVLGQTLIEQEGKDLFEAEEELRLLCKRLRFEYDPVLDERLRRRIDA
                     MSVEDLRKIVRAFSVYFQLVNIAERYHRVRRRRQYESSPDNPPQRASVASALSRLKKE
                     GLGQRKLRSVLDSMSVGLVLTAHPTEAMRRSVRRKHERIGEMLESMESPALTWKERRG
                     LEEELAEEITILWQTDELRVRRPEVNQEIERTLLFFENPLISATVEAYREFEDELSRL
                     FPEDTPSLGRILEFGSWVGGDQDGNPFVEPEMITAALGLHREVILKRHLASVLWLADH
                     MSQSARMIPVSEELRRSVERDVELMPEMVERYRGVDPNELYRRKLLLMSERLRRTLQR
                     SDSLTAYPGVKDFVDDLSTIRDSLMNNGGERVAEGA"
BASE COUNT         2436 a         4313 c         4014 g         2334 t
ORIGIN      
        1 ggctatccca ctcattccag ccccgccgat gccgatcatg tggatcttca tggtcctcac
       61 tccttctctc cggcacgcag cagtctttcg gccacctcgg cagccgctcc gggggttgcg
      121 agatcgccca tgcgtctggc aaacgtctcc cgccgttcgt catcatcgag caagtcctcg
      181 acgcgctcgc gcagcgcctc cgccgtgacc tctgagtcga gcatcaactc cgcggccccc
      241 ctctcgacaa agtacctggc attgtggagc tggtggtcac ccgtcgcgta ggggaatggg
      301 atgagaatag ccgccttccc ggcggccgcg atatcgaaca gcgagcccgc gccggcccgg
      361 ctgacgacga cgtcagcggc ggccagatac cgccagatgt cgtccgcgta ctccaggatg
      421 cggtgcttcg ggttgtccgt ggagagccgc gcgaagtccc tgcggcccga cacctggacg
      481 accgtgtacg aggtctcgcc cctgaacgcc tccgcggccg cgaggttgat cctgagcgca
      541 cccccgctgc caccgaagat taagacgacc ggcggctcca ggccaagctc ccgcatcgcc
      601 tctttgcgcg aagcatcgaa gacctcgctc ctcgtcggca tcccgaccca tactgcaccc
      661 ctgagcagtc gcgccgcctc aggaaacgta accagcgtct ccgtcgtaac gcggctcgca
      721 agccggttga cgcgcccggg gaccgagttc tgctcgtgca gaaacgtcgg tactccgagc
      781 gtccgggccg cggtcaccgc aggcgcgctc gcatatcccc cgacgccgag tacggccctc
      841 ggtgcgagcc gttgtattat gcgtctacaa tgcctgatcg ctttaaggga gaggaggatg
      901 gcgcgggccc gggcgatagg gtttcccgcc agacctgtca ggggcagagt gtggagagta
      961 aagccggcgc tcgggacgag ggtactttcg ataccggact gggatcccac gaactcgacc
     1021 aaggctcctc tctccctcaa atcagccgcc acgcacagcg cggggatagc gtgccccccc
     1081 gtgccgcctc cggcgatcag gattcgtggc cttgtgtggt ttgatctctc tggctcgttc
     1141 actgtcctcc gatatcctgt agaggatgcc cacggcggcg aagcatacca ggagactcga
     1201 accgccgtag cttacgaagg ggagcgtcat gcctgcgagc gggaggacga ccatggccgc
     1261 cccgatattg aagatcgcct gtacggtcag catcgtggtc aggccggcgg cgacgcacct
     1321 cgccatgacg gaaggggcgt tcatggctat cctcagcccg gcgagcgcga tgaaaccgaa
     1381 ggcgcagatc acgacgacca ttccgataag tccgagttcc tccccgatca gcgcgaagat
     1441 catatcggtg ccgagctcgg ggacgtaagg tccctgtccg ccggaacctg cccctgcccc
     1501 gaagaacccc ccggctttta tggctaccat cgactggact acctggtagc ccgagccgtc
     1561 cgctgcagcc cagggatcga gaaacgtcat gaaccgctcg cgccggtaag gggcgagcaa
     1621 catcataccg atcaccgcag ccaatcctgc gagtcctgag agaaccaggt ctttcgtcct
     1681 cagttcggat gcccagagga cgccggctac gccggagagc aggaccaccg aggtgccgaa
     1741 atccggttcg accagtacaa gggcgaaaag caccccggcg gccactacgg gcttgacagg
     1801 cagcttcgaa ccacgcggca gacgcgcgac cgcgcaactc agggcgagca cggccgccag
     1861 cttcgcgaac tccgcgggct gcacgctcat gggtccgagg tcgagccacc gtcgcgcacc
     1921 gttcacctgg tttcctatcc ccggaatgag gacgagcacg agtccgacca gcacgatcac
     1981 gtacagagcc ggagcatatc gccgccatgc tgtgtacctg atccggctcg ctgcgaagaa
     2041 ggcggccacc ccgagcacca tgtgaccggt ccgcacgaac agatactcag tcccgacctc
     2101 cctgtaggtg gcggagtaaa ccatgacgag tccgagcaag gagagaccca gcgcaacgag
     2161 gaacaggttg gcccgcaaac tactcatcgt ccgacggccc ctccggtgag ctctcgcgcg
     2221 aacctggtga aggcgtctcc cctctcggca tatcccggga actggtcgaa gctcgcgcaa
     2281 ccaggtgaga gcaggacgac gtctcctggt cttgcgaagg atccggcctc ggcgacggct
     2341 ccctccaggt cgggcactct gtggacgacg tcctgccagc cctcgtcttc gaggtatgag
     2401 gcgatgcgcg gtcctgcctc gccctggcag atgaccgccc ggcatgctcc gagtccaggc
     2461 atcaattcag agaaatcggt gtccttctcc gagccaccga ggatgagcac cacaggcacg
     2521 tcgaagctgt ccagagcagc cgctgccgcc gcagggttgg tggctttgga atcgtccacg
     2581 taggtcacgt acccgcgctc ggcgacgacc tgcatccgat gaggcttgag cttgtatcca
     2641 agcaacccct ccctgatttc gggaagaccc gcaccgagcc tctccgctgc cgccgccgcg
     2701 aagagggcgt tctggtagtt gtgcaaaccc gcgaagtgca gctcggctgt gtcagcgaca
     2761 tgggagccgc gaaggaggag acgcccgtcg ttgacggaag tatcgccatc gcccacgatg
     2821 agcgcctcgg cctcgaggtc ttttagtgaa ccgcttcctg cacggtcttc ggtgttcagc
     2881 agggcgagat ctccagcctg ctgtccatcg aagatgcgga gtttgtcgcg aacgtactcg
     2941 tcgaaagagg cgtgccagtt caggtgatcc ggcctcacgt tcaacaaagc cgcgacctcg
     3001 aagccggggc tctgaaggta gtggagctgg aaggaggaga cttcgaggac gaggattccg
     3061 gcattacgcg cctcttccac acaccccgtc agcgccctcc aggagttgcc cgccacggcg
     3121 tggcgcacgt ccgccgcgtc tagcatgcgg tgcaccatgt ccacaacggt cgtcttgccg
     3181 ttcgtgcccg tgaccgcaac cacgcgggca tcatccccga gcaggtccag gccgagtcct
     3241 acctcggaga cgacggggac gcctcgcgct tctgcggcct gaagtacggg gtccgatggc
     3301 ctaattccgg ggctggctat cacgcggtca gtcccttcga gaacctcagg gccagcgctg
     3361 aggaagcctg atacgtcgag cccggcaagg acgtcccgca ggtgttcgtc gtctcccgca
     3421 tcggccacga tgacctcctc gccacgttcc accaaagccc gcgtggccgc gacgcccgat
     3481 tcaccgagcc cgtatactag agtcttcacg ggctcacccg accgcgttgt agaggaagat
     3541 atagtagagg aagaagccga cggatgagaa cgcagcctgg gcgatccaga accgtaccac
     3601 gatcttattc tcatcccagc cgaggaactc gaagtggtgg tggatcgggg ccatcttgaa
     3661 cacccgccgc cccgtgagct tgaagatgaa gacctggatc atgaccgaga ccgcctccac
     3721 tacgaacagg ccccctacaa tcggcaggag tagctccgtc ttcgtgagca ccgctgctgc
     3781 cgcgaggaca ccccctatcg caagagagcc cgtatccccc atgaagatgt ccgcggggtg
     3841 ggagttgtac cagaggaacc cgacgatcgc cccgaccatc gcgccgcaga tgacggcgac
     3901 gtcgtactgg cgctcgagga aagcgatggc cgtgtatgcg agcagggcta tggcggcggc
     3961 ccccgccgcg agcccgtcga ggccgtccgt caggttgacg gcgttggtgg tgccggcgat
     4021 cacaagcaaa aggaagatac tgaagagcgc aatcccaacg actcccggtc ccaataccag
     4081 gctacggtcg aagtaaggca cgagaacggt ctgggtgacg ccgacgtagc gcacggcaag
     4141 gacgtcggca aggacgaccg ccagggtcag tagcaggaac ttgtagcgcg cgctcaaccc
     4201 ctccgggcgc tgcctggaga tcttctgcca gtcgtcgtag agaccgatgc cggcaacgac
     4261 cgcgacgagc aagagcgtgg tgagcgtggc cacgttgagc cgcgcgacga ctaccagcgc
     4321 cccgaggaga cccgtgagca tcacgatccc ccccatggtc ggggtgcctg ctttgatcag
     4381 gtgcgtctga ggtccctctt cgcgaacgaa ctgcccgaac ttccgtgact ggaggaactc
     4441 gatgaacttc gagccgagca tcacggttac aaggaacgct accgccgcag caagtacaac
     4501 gctaaacaag agctaaactc tccctcaact tacgggtcag ggcatcgagt ccgactccgc
     4561 gtgaaccctt gacgatcagg tagtccccgc tcctcagttc gtcccgcagg gcttcagccg
     4621 ccgtctcggc gtcttcgtag aagagggact tccctgggaa ggcctcggcg taccagcggg
     4681 cctcgtcacc gacgcagacg agaacgtcga cgccgacttc ccctgccagt tttcctgcct
     4741 cctggtggta ggcgcgggcg caggcaccga gctcgaacat tcccccgagc acaacgatga
     4801 gccgtctacc ctgcctggct gcctgatccg caccgtacct caataccgcc gcgacggcgg
     4861 caggtgaagc gttgtacgtg tcgtcgtaga ccgttatgtc ttccttgagc cggtaaacgt
     4921 cgccgcggag gcccgtacgc cgcagacggg agaggccgcg ggcggcctct cccaggccga
     4981 ggccgagcgc gagcgcgccg ccgaccgacg ccagcagcgg ctcgaccagg tgcgtcccga
     5041 aaaccggcga atagacctcg gcggaccgtt cgaggaggtg ggcgacaaag tgcagacctt
     5101 catccctgtc ttcgacgtgc gacgcgaaga ggtcggcgtc ggcgtcgcgg gcgaaagtaa
     5161 tacgcttcag ttttctccct gaccctgtcg ccgcctcggg cgcgcctgca ggggcgacaa
     5221 gggttcccga gtcgggaagg gcgagtgcca gctcgccctt cgccgcggcc agcgcttcga
     5281 tgctcccgaa agagtcaagg tggacgggtg agatcgcggt tagaacaccg atctccggag
     5341 gcgctatgga gcagaggtac tcgatgtccc ctgcatgggt tgcgcccatc tcgaggacga
     5401 ggacctgggt gcgcgggtcg gaggtcaata ccgtgagggg gagaccgatc tcgttgttca
     5461 ggttcccggc tgtcgctgag acacgctttc cggcgtgcct gagaatggtc gcgagcgcat
     5521 ccttggtcgt tgtcttgccg acggtaccag tgacgcctac aacggcaggc gaatcctcgc
     5581 gaagcagcgt ccaccgcgcg agccgctgca tcgccttcag aggatcgccg acaacgaggg
     5641 tcggcacgtc gagacggcgg gcggcgaccg tcgccacggc gcctcgcagg tgcgcctcgg
     5701 gtgcgaagtc cgcgccgtcg gagcgccctc gcaaggcgaa gaacaggtcc ccttcacgcg
     5761 ccttcctgga atcgatggca gccccgccga caagggcgct cccgtgcgta gctgggaggt
     5821 ccgcgccaat agcccgggcg acctcactga gagaagctgg cctcactgac tcgcctcctc
     5881 ggcctcaaaa cctctccggt ccggcgggac gttgaagtag cccagcgtaa aagacattac
     5941 ctggtggaaa gctggtacgg cgaccagctc tccccagtat gaggtctgtg gttcgtcgac
     6001 cgcgatcagg gtcacgtact ccgggtcggt agcgggggca aagcctataa aagaggccac
     6061 gtactggtca ccgtaggttc cggtcttcgg gtcgactttc tgcgaggtcc cggtcttacc
     6121 gcccaccgtg taaccggaga tctgggcgaa atggcccgac ccatcgtcga cgacgccctg
     6181 caacatcccg cgtactatag ccgaggtctc ctcgcttatc acacgtggtc ctggtgtctc
     6241 ggggttctcc tgttcactta cgtgcggcgt gaccttgagc cctccgttga tgagggtggc
     6301 gtagccggcg acgagctgca acggcgtcac ggtaaatccc tgtccgaagg gaatgttgcc
     6361 gatggagaca ccgctccagt cctcgtaggc tgggacgctt cccgggtcct cgccccacag
     6421 gtcaacgcct gtcggttctc cgaatccgaa ggcacgcatg tactcgtaga gcttcagccc
     6481 gcccagctcc agcgcaacct ttgtcgcccc gacgttgctc gactgcttca gtacgtcggc
     6541 gggggtcatg atctcggtct cgtgcggctg tgagtcgttg atcacacggt cggcaacggt
     6601 catgtggtcg gggacgacga aggtgctctc cggtgtgacg gccccctcct cgagcgccga
     6661 agccacggtg attggcttga aggtggatcc cggttcgtaa gggtcggtga ggacccggtc
     6721 gcgctgggct tccgccgaca cgttcccgaa tttgttgttg ttgtaggccg gcgtattggc
     6781 gagcgccacg atggccccat cgtccacgcg catgaccaca ccgacggcgc tcctggcccg
     6841 gctcttctcg acagcagcat cgagggccgt ttgcaactcc tgctgcacag cggtatccag
     6901 ggtgagcgtc acgtcatcgc cgctcttcaa cctctcatcg tacctcgcct cgaccccgcc
     6961 gaagactcca aagtctccct ggtgtccgat gagctggctg gcgagggacc cgtcagggta
     7021 gaccctgagg gtgtccggag ctatggtgat gccctctatc cccaacgcct gcaccctcgc
     7081 ggctgtctcg ggtttgacgt ctgtggcgac gacgctgtat cccgtcaacc ggccgctcgc
     7141 gtcacgtttg gcaagtgccg cctgtagctc atccagactc cgatccgtcg cctggccgag
     7201 aacggcgtct aacttcctgg ccgtcgcgtg cggatcctcg acctgataag gggtcgcgac
     7261 cacgcgggcg acttcgaggc tcgtagcgag ctccctgcca tcggcgctca ctatgctccc
     7321 gcgtgtgggc tcggcgacct gcgcctctcc cccgacctgt tcgtcggcga acgcctggta
     7381 actctcgtcc tgcgtgaggc ttatgtgcac ggcccgcccg ccgaggagta gtcctgtcac
     7441 cgcgaacact gcggccacca tccatagcct cctgtggccg atagcatgcg tttcgccgct
     7501 ggtagaagac tctcggggcg tcctcttgac gcctcgggtc cgcccctgtc tggagatcgg
     7561 tatgaccttg ccaccggccg accggcggtc tcgtgttttg gtgcgaaggg agttcttggc
     7621 gccgcggaca accttcgagt ttcgaggccc tggcctgtca acgctgccgc ggtttccccc
     7681 cttcctctga tgccccatcc tgtgtcccat cctccgcttc gtttgcatag accttcaact
     7741 cgtcgctccc gggctcgtgc atgccgagcc tctcggccgc aagctcccgt atcctcccgg
     7801 cacccgacag ttccgcgacc ctgaccttga gtctctcccc ctggtctcct gccctgtcaa
     7861 gcttcacgcc gagttcggca gcccttgtgt cgagtcccgc gccgaccgtg tgcagataga
     7921 cgctccccag catcaatagc acaggcacaa cgacgagcat cgcaaaccgg cgcctccggg
     7981 cggccgcctt ccgcaagtga gcgcggtccg gcgagcggtc cggggcgtgc ttccgtacgg
     8041 gccacgccgg ctcggtaggg agcgtagcct ggaacctgcg ctgcgacgcg gccatcaggt
     8101 accatccgaa ggcggctcgg ccgtccttat cgctgaacgc aaccgggccg atgcactgcg
     8161 cgggttcgcc tctacttcac ccatcgaggg cgtctgaacc gcgtcccgcc ggaaaacggg
     8221 ccgcgcaccg cagacgcaga ctggaagctc tggcggacag acgcaccgcc cctcccgctc
     8281 ggagatgaag cgcttgacga gtcgatcctc cccggagtgg aaagtaatga cgaccaacct
     8341 cccacccgac gtgaggagcc gctccgacgc ctccagcgcc cgcccgagac cgcccagctc
     8401 gtcgttgacc ctgacccgca cggcctggaa gatcctcttg gccgggttgc cgcctttctc
     8461 cttccagccc acggctgccc gcacggcctc gtacagatcc gtcgtcacct caagcggtct
     8521 tcttcgaacg acctcccgag ccacacgccc ggcctcgccg cgcggcacgt ccccgtactc
     8581 agagagcact cgcgccaact ctccgacctc gaccgtgttc agatactcag ccgccgacac
     8641 ccccgacccc gggtccatgc gcatgtccag cggcccctca cggacgtaag agaatccgcg
     8701 ttccggttcg tccacctgaa aactggagag accgaggtcg aacaggacgc catcagcccg
     8761 tctaccttcc tccaccatct cccacaacgc ttcgtcaaat gcaccggaga cgaactcgaa
     8821 gcgcgggtcc tgaagaagcc cgctcgcccg cccgatcgcc tcagggtccc tgtcgatacc
     8881 gacgaggttg ccctcagggc ctagctcgtc gagtatccgc agggagtgac cgccaccacc
     8941 gaaggtggcg tctacgacag catcgtccgg acccagccgg agggcggaca ccgcctcctc
     9001 cagcatcacg ggacggtgtc cggtcgccat cacccgccga gatcccttcc ggcaaactcc
     9061 tccacgatct cggagaacga gtcgtccgca ccggaaacgt agcggtccca ctcctctgtg
     9121 tcccagatct ccagcctgtc gtccacccca gttatggtca cgtcgcgctg caacccagcg
     9181 tagcgggcga gcttcgttgg gatcaaaacc cgaccctgcc ggtcgagcgt ggcctcgaaa
     9241 gccatggaga agaacatcct cgaaagctgc cgacctctcg ccgagaggtc ggcgttggcc
     9301 ttgatgttgg ccttgaacgc cgcccactcc tcaggcggaa aggcgaatag acacctgtct
     9361 acccccttgg tcacgacgat accaccttca aaatatggtc ggagtcggga ggggagggtc
     9421 attcgccctt tatcgtccag cgtgtgctcg tactcgccta aaagggccag agccgtgtcc
     9481 tcccactacg ctccactttg acccacaaca acgaaattct tgcatatgaa gggaaaatta
     9541 gcaatcaaga agcccgaaga cgcctcagat gggccgtatt ttgcgaatga gaagccgctt
     9601 atccctctcc cctagggata aggaccccta taaatttagc tctgttggct ggcttgcggg
     9661 cattcggtcg acggggtggg ggaagatccg gggcgtaaca tgttggttgc gcgaaatacc
     9721 aaccccaaca tattggggtt ggtcaagaaa tgtgtggctc gccttgattc agagaggtga
     9781 tgaccaagcg atgtggcttt ggtgggccca ccaggactcg aacctgggac cgtccgatta
     9841 tgagtcggat gctctaacca actgagctat gggccctcag cgggaaacct ggacggtacc
     9901 gcctagctcc cccggctgga ctcgaaccag caacccttcg gttaacagcc gaatgctctg
     9961 ccaattgagc tacaggggaa tgccgcaaga tgagattata cggttcgggg ctcgtcgctt
    10021 caagctccgg ctgagacgga acgcagggcc accttcagct ggtgaatctc tgactggagc
    10081 gactccttct cgtcataatc cggggtctcc cgcttacgtc gttcgcgact cagtatcccg
    10141 agtcgcaacc aggcttcgcg cacggagctt tcggacggga agagcctctc tccctgggag
    10201 gcgagggcgc cgatctgatc catcagcggc cttgccctct catccgagag cacggcatcg
    10261 agatcttcgc ccccatgttc tttcaggacg ttgaagatcc tggcgtagac gtcgtcacga
    10321 aaatcctcgg tgcgcagcgt gaagggctcg gggagtgcct gagtctggat accgttctcc
    10381 agaaggggtg ccgtgaggtc aggtctggcg agaacgaggg ccagtacggc gccaccagct
    10441 tccgttagag ggtcggtctg gcgactgccc cgcggagagc tcgcccgtgt agtggcgggt
    10501 tccgacgtct gcagcacctc ggggttcacg ccgagagatt ccgtggccag gcgcacggcc
    10561 tcgcggtaga ggacggggtc tttgatctcc tggatcatgc tcttgatttc ggggacggct
    10621 ttggagcgct ccgtagcatc cgctccccgc gtacgggcga cgatgcgccg gatactgtac
    10681 tcaaggacgg gcacggcgcc tttcaacatc tccgcaaact cctcgggcgg atgttcgagg
    10741 agccagtctg ccgggtcatc aggcagacgc atgactcgga gatccaactt caaatcagcc
    10801 gccgtcgccg ccgccctctc gatggccttc tcccctgctg cgtcagggtc gaagagaacg
    10861 tagatcctat ccgcgtagcc actgagcgta cgcaaatgcg cgggggtggt cgccgtgccc
    10921 aatgtcgcga ctgcgttctt gatgcccgac tggtagagca tcaagacgtc cgtatagccc
    10981 tcgacgacaa gcgcggctcg ctccctgcga atagcctcgg agacctgcgg aaacccgtag
    11041 agaaggtctc gcttgttgaa gatctccgtc tccggagagt tcaggtactt cggttgggcg
    11101 tcaccaagcg tcctggcccc gaagccaacg atcctcccgc gccggtccga gatggggaag
    11161 gtgatcctgc cggcaaaacg ctctccaccc cgcgacgaaa gaagcccagc cgcattgagg
    11221 acactcctgt cgagattcag cttcctcgcc acccccaaaa accccgaaga accccgcggt
    11281 ggagcgtatc ctattcgaaa ttcttctata gtagaacgtt ccagatggcg gtcgcgcagg
    11341 tagcggcggg cctgttccgc ctcgggggtc tgggacctca ggaagtactt gtggtagtag
    11401 acggcggcgg cggccagtgc cttgtggatc tcgcgtcgac ggagagtacg ttcccttgcg
    11461 gcgccggggt ctcccccacc ctcgaactgc acgtccacgc cggagcgttc tgcgagatac
    11521 gcgacggctt cggcgaagtc gaaggacttg aggtccatga cgagtttgat cgcatccccg
    11581 ccccgttggc agccgaaaca gtagtagaag ccgcgatcag gggagacgct gaaggaaggg
    11641 gttttctcct ggtggtccgg gtaagggcag agacctgcga accgggctcc ctggcgcttg
    11701 agggcggtga actcggaggt tacctcgacg atgttggccg cctccctgac ctcctctatg
    11761 ctgcggtctg agatcctcaa taccggtcct ccgttgtact tatgtacaaa tatatgcgtt
    11821 ccgtcgtggt tcagaacaat ggtaatggta ctctaccgga cgtggacgtc cgcgatagat
    11881 ctacgcctgt acccgagtcg gggttcgctg acctgccgcc ggggatcggc ttcgaatcca
    11941 aagacgagcc gctgcgcagg gacatcaacc ttctcgggcg ggtgctcggt cagaccctca
    12001 tcgagcagga aggcaaggac ctcttcgagg cagaggagga actgcggctc ctgtgcaagc
    12061 gcctgcgctt cgaatacgac cccgtgctcg acgaacggct caggcgacga atagacgcga
    12121 tgagcgtgga ggacctgcgg aagatcgtaa gggcgttctc ggtgtacttt cagctggtaa
    12181 acatcgccga gcgctaccat agggtgaggc gaaggcgcca gtacgagtcg tcgcccgaca
    12241 atccgccgca acgcgcctcc gtggcgagcg cgctctcgcg gctgaagaag gaagggctgg
    12301 gacaacggaa gctgcggagt gtgctggata gcatgtccgt gggactcgtt ctgacagcac
    12361 atccgaccga ggccatgagg agaagtgtca ggcgcaagca cgagaggatc ggggagatgc
    12421 ttgagtccat ggagtcgccg gcgctcacgt ggaaagagcg aaggggcctc gaggaggaac
    12481 tggcggagga gatcaccatc ctgtggcaga ctgatgaatt gagggtccgc cgccccgagg
    12541 tcaaccagga gatagagcgg acactgctct tcttcgagaa ccctttgatc tccgccaccg
    12601 tcgaagcgta tcgggagttc gaggacgagc tgtctcgcct gttccctgag gacactccga
    12661 gcctgggtcg catcctcgag ttcggctcat gggttggtgg cgaccaggac ggaaaccctt
    12721 tcgtcgagcc cgagatgata accgcggcac taggtcttca ccgcgaggtc atcctcaagc
    12781 gccatcttgc ctccgtactg tggctggcag accacatgag ccagtctgca cggatgatcc
    12841 cggtctcaga agaactgcgc cggtcggtcg agcgggacgt ggagctcatg cccgaaatgg
    12901 tcgagcgata cagaggcgtg gacccgaacg agctctaccg ccgcaagtta ctcctgatgt
    12961 ccgagcgcct gagacgcacg ctgcagagat cagactccct cacagcctac ccaggcgtga
    13021 aggactttgt ggacgacctt tcgaccatcc gcgacagcct gatgaataac ggcggggagc
    13081 gcgtcgccga aggggct
//
LOCUS       DATAIR010000318         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_148046, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000318 DATAIR010000000
VERSION     DATAIR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148046"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VHM69_07770"
     CDS             <1..577
                     /locus_tag="VHM69_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740329.1"
                     /translation="AARFRIDRVSGNPAILDEKKLRSVNALYLRRKSPEELAALAAPM
                     LVESGAVTEEELDMPRLREIMDLLKERIALTTEIPDSVGYFYGGTLDYDEAEFEKQFG
                     KEFVKENFPELVERLAALPEWTEAAIEDAIRGLAAEKEKGARHLIHPLRFAVTGRTVS
                     AGLFETMQLLGRERSLLRAGDAAQKMQRLPV"
     gene            642..714
                     /locus_tag="VHM69_07775"
     tRNA            642..714
                     /locus_tag="VHM69_07775"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:676..678,aa:Glu,seq:ctc)
     gene            complement(747..1286)
                     /locus_tag="VHM69_07780"
     CDS             complement(747..1286)
                     /locus_tag="VHM69_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740330.1"
                     /translation="MTNALNPFVIFTGLYALVALAEAGVSRGFLYLALELLAAVAVAG
                     YVVLMRRRSRVGDFWISARAERLIPALFLLAAFVALLATLSLFDAPQNLFLLTLSMGM
                     ASAAVALITLFWKASAHCTVAGHAAAAGLLLLGPLGIVFLLALPLVVWSRVVLKAHTL
                     PQALAGAAVGAGVAILFLV"
     gene            1318..>1790
                     /locus_tag="VHM69_07785"
     CDS             1318..>1790
                     /locus_tag="VHM69_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740331.1"
                     /translation="MVGRIGIVQPPRTLTDEETLALWYELGRLYSESGGGGQRATKLG
                     FTVVCAVGALVLLSAPLFGTAWAGPFAAAIPVMAGLLSGGGLYLRQRSRLRRRADILR
                     TRLAERGLDAERPARDGLGAYYDAQLILLRSEYEYLRVRGVHKTARLFEESFGFTP"
BASE COUNT          353 a          555 c          607 g          275 t
ORIGIN      
        1 ggccgcacgc ttccgcatcg accgcgtgag cggcaaccct gcgatcctcg acgagaagaa
       61 gctcaggtcc gtgaacgccc tctacctgcg gcgcaagagc cccgaggaac tggccgccct
      121 cgccgccccc atgctcgttg agtcaggcgc ggtgactgag gaagagctgg acatgccgcg
      181 tctgcgggag atcatggacc tcctgaagga gcgcatcgcc ctgaccaccg agatccccga
      241 ctccgtcggc tacttctacg gtggcaccct cgactacgac gaagccgagt tcgagaagca
      301 gttcggcaag gagttcgtca aagaaaactt ccccgagctc gtcgaacgcc tggcggccct
      361 ccccgagtgg acggaggcag caatcgagga tgcgatacgc ggcctcgctg ccgagaagga
      421 gaagggcgcg cgccacctca tacatcccct ccgcttcgcc gtcacaggac gcaccgtcag
      481 cgcgggactc ttcgagacca tgcagctcct cggtcgcgag cgcagcctgc tgagggcagg
      541 ggacgcagcc cagaagatgc agcgccttcc cgtctagacg ggcggaaccc ttttgacgaa
      601 caggtgaccg tcgggtagaa tcgtcccctg cctaacggca aggccccatc gtctagaggc
      661 caaggacatc gccctctcac ggcgaaaacc cgggttcgaa tcccggtggg gtcactctcg
      721 caagcccgct catcggcggg ctgctttcat accaggaaga ggatcgcgac ccccgctcct
      781 actgccgcgc cagcgagcgc ctgcggcagg gtgtgagctt tgagcacgac ccgggaccag
      841 actacgagcg gcagcgcgag taggaagact ataccgagcg ggccgaggag caggagtcct
      901 gcggcggcag cgtgacctgc gacggtgcag tgggcgctcg ctttccagaa cagtgtgatc
      961 agggcgacag cagcactagc cattcccatc gagagggtca gcaggaagag gttttgtggc
     1021 gcgtcgaaca gggataaagt cgcgagcagc gccacgaagg cggcgagcag gaacagggcc
     1081 gggatgaggc gttcggcgcg ggcggagatc cagaagtccc caacccgcga gcgccgacgc
     1141 attagtacta cgtatccagc cacagccaca gccgcgagca actccagagc caggtacagg
     1201 aagccacgcg agactccggc ctccgcgaga gcgacgaggg cgtagagtcc cgtgaagatg
     1261 acgaaagggt tgagagcgtt cgtcacggcg cgggcaaatt cctgagggcg gacggtcatg
     1321 gtgggtagaa tagggatcgt gcaacctcca cgcacactga cagacgagga aaccctcgct
     1381 ctgtggtacg agctgggacg cctgtactcg gagtcagggg gtggtggaca gcgggccacg
     1441 aagctgggct tcactgtcgt ctgtgctgtt ggggcgctgg tgctgctctc cgcgcccctg
     1501 ttcggcacgg cgtgggcagg acccttcgcg gccgcgatac ctgtgatggc cgggctgctc
     1561 tcgggtggcg ggttgtactt gcgtcaacgg tcgaggctac gaaggagggc agatattctt
     1621 cgaacgcggc tggcggagcg cgggctcgac gcggaacgtc cggcgcggga cgggctcggc
     1681 gcatactacg atgcgcagct aattttgcta aggagtgagt acgagtacct gcgcgtacgc
     1741 ggcgtgcaca agacggctcg cctgttcgag gagtctttcg gctttacccc
//
LOCUS       DATAIR010000319        11933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20352, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000319 DATAIR010000000
VERSION     DATAIR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20352"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHM69_07790"
     CDS             complement(<1..225)
                     /locus_tag="VHM69_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740332.1"
                     /translation="MAISGALSVVRRGGVSAIRRAIDGALGRLRAGMWPVVQTAVAAT
                     LAWSAAALVLGHEQPFVASIAAVISIGAVAG"
     gene            complement(476..1825)
                     /locus_tag="VHM69_07795"
     CDS             complement(476..1825)
                     /locus_tag="VHM69_07795"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HEX2740333.1"
                     /translation="MNDLRQVHRREPHPRAMNGDAPDPRRLYTRRGFLRVAAMGVAGT
                     SCGGLFTACDDSSLAGKLRVTPNSVRDDEPFTIILKDLSPWQRVTLSARFFDGYDVEW
                     TSTATFRADFLGTVNVSEQAPLGGSYTGADPMGLVWSADGMGTSYAISLRPRPLFITA
                     ETDGRKAGVRVVRHLLTSDIESTDVRARGLYGKFFRPATGEPAPGMLVLGGSDGGLAS
                     YVMREAALLAHHGFAALALAYFYFGPLPDRLARIPLEYFGKAIGWLKDQPAVRGDRLG
                     VMGTSRGGELALLLGAHYPELGAVVSYSGSGLVFPSPDSREPAWTFRDEPLPWIPNPF
                     DILQATPDQLERAEIPVERTNGPVLLISGDADQVWPSTQLSQVAMDRLQRFERHYLDG
                     FLHYPDAGHGIQPPYLPATPGTYYNGGDPKGNAFANEDSWQRVLNMLDERLRRTTGA"
     gene            complement(1889..2923)
                     /locus_tag="VHM69_07800"
     CDS             complement(1889..2923)
                     /locus_tag="VHM69_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX2740334.1"
                     /translation="MTTVMITSYFEEEHIRRIREVDGRLRVLYREDLVPPPRWPGDHA
                     GPEGWRRTPEGEEEFLAMLGEAEVIYDFPRGHVGDLHEVAPKLRWVQGSMAGAGEVAQ
                     KAGLGDTAVVVTTASGIYSGPLAEFVLMAMLQHAKGLDRLRREKAERVWRQGTTGTLE
                     RKTLCVVGTGSIGRAIAQRARPFGMRVVGVKRTVREDDAAWQYADELYATADLRKALG
                     EADYVAVTLPGTPETRHLLDADAMASIKPGAYFANVGRGTVVEEAALVEALESGHLSG
                     AALDVFEVEPLPDESPLWDLDNVIVSAHTTDVVPDLINPAQTDLFCENLRRYLAGEEL
                     LNVLDKRLLY"
     gene            3012..3641
                     /locus_tag="VHM69_07805"
     CDS             3012..3641
                     /locus_tag="VHM69_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2740335.1"
                     /translation="MEELLDILDESGRRTGEVVPKSEAHRRGLWHRGFHCWICGTDTT
                     GESYLLIQRRSATKDTWPGRLDITAAGHLTAGEGTLDGLREVEEELGLRVEPERLIPL
                     GSRRAEQEIPGGYDREFHEVFLLVDTTPPERMRLQVEEVDALIRIRLDLVDALGNGES
                     VPAKEYADGKTVATRVYPSDFVPNSDDYLRRVAGAARRVLAGEEPGRVF"
     gene            complement(3638..4072)
                     /locus_tag="VHM69_07810"
     CDS             complement(3638..4072)
                     /locus_tag="VHM69_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740336.1"
                     /translation="MVARKCTPADAGAMAAGLLVDACGPSPNNSLRMEVVRELALDLG
                     RRLEALSDSEETDALIEAAQACSDLATLAACNIQDLTEEGRPLATAAVHLAAGATRAL
                     ISLSESKTGSRDESHAENTHRDARSAGWRADLAVRQIEDPQD"
     gene            complement(4099..4947)
                     /locus_tag="VHM69_07815"
     CDS             complement(4099..4947)
                     /locus_tag="VHM69_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX2740337.1"
                     /translation="MARNQLRGEEVKEHAAEAARHASPWVERLARLGYVAKGVVYAVV
                     GFLALREALGAGGQTTDPSGAMQSIGTQPLGGIMLALLAVGLACYALWKVVQGITDPD
                     GKGSDAHGIMRRVAYVGSGAIHGALAYTAAQSILGAEDSSEDAAAASAMAFQPPLGQI
                     LVGLVGMAVIGVGLYQLYAAYEAKFRADLRLGQMGEAEERWVIYAGRIGTAARALAIG
                     VAGAFLVLAAYQSDPSETRGLGAALETVQHQPLGSYMLSAIAAGLLIYGAFMFAIARY
                     RRIYSA"
     gene            5036..6760
                     /locus_tag="VHM69_07820"
     CDS             5036..6760
                     /locus_tag="VHM69_07820"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX2740338.1"
                     /translation="MRVALAQINTTVGDIWGNVEKMSDTLGRATDAGAELVAYPELTI
                     PGYPPEDLLMRPSFIEENLRALDEFAGKVPEGVVAAAGFVDLDADLYNSCAVLSGGEI
                     LHRYHKRYLPNYSVFDENRYFREGSGAPIVELGGALVGVSVCEDIWYPGGPAREQALG
                     GASVLLNISASPYHRRKGAFRERMLGVRASDYGCYVVFCNLVGGQDELVFDGHSVVFD
                     PEGGLVARADQFKEDLLIVDLYPDHSLMQRLHDPRPRKENVERPPQIVKVPDYGPRSV
                     EPVEARVEPYLSEEGEVLEALVLGLGDYFRKNGFSRAVLGLSGGIDSSLAAAVAVEAL
                     GPQNVTGVLMPSRYTSEVSNTDAAALAKNLRIDTQTIPIGPAFDAYREMLKSAFKGLP
                     EDVTEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDSAGGFAVIKDV
                     PKNLVYRVSRHFNERKDREVIPDSILTKEPTAELREDQRDTDSLPPYEVLDPILEAYI
                     EEDKGVAEIVASGFDEDDVRRIVQLVDRAEYKRRQAPTGIKVTGRAFGRDRRMPITNR
                     YFERNAEE"
     gene            complement(6767..7354)
                     /locus_tag="VHM69_07825"
     CDS             complement(6767..7354)
                     /locus_tag="VHM69_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2740339.1"
                     /translation="MHGISLTLRAALDTERRGDREEAHEMFERALGVSREAEFQISRP
                     YDELQTLRSENRPRAGDFVRQRLMKFEEYFGIKANDDLQAPLEGLDQDEIAAFIRVFV
                     EACWNVAKHSGASNLYLESRHVGSVLIIRVRDDGRGFDTGEPLPGLGLQYMRRRAGEV
                     GAELDVISTPGRGTTVQMRFSTHKNAPDTTGSAEV"
     gene            complement(7427..8410)
                     /locus_tag="VHM69_07830"
     CDS             complement(7427..8410)
                     /locus_tag="VHM69_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740340.1"
                     /translation="MQSSEAGKLRDPRAPRIPATVALVCWAAGALVFASLPQGSDAKS
                     VIATIFSLSSATFAAVILLRAAPRAQGHERAFLRLMGWGMVFRLAGNSVWSTSQLLDL
                     GIVRPVAPQDVAYAVSYTLLVGAMLHLVALATRRITLVSAIDAGAVMLSVGTLAWYFV
                     LGPAEPGSVRDAVVALSQPVCDAALLFLGLVVASSPARPRFSAFLNGGFVALLLADAA
                     YLGLRSAGPYQSGNWPEMLWALGMILLGLTSTTSPEVPADPNVRLIQPWRILLFWLGP
                     LSPPVQFTILLLLGVINPPLPAYVLTGCAVLLLYMAARIGLVSFVSRNLSQ"
     gene            8527..9522
                     /locus_tag="VHM69_07835"
     CDS             8527..9522
                     /locus_tag="VHM69_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740341.1"
                     /translation="MEYRRLGNTGLLVSELCLGCMTFGQEADEETSKEIVGRFLDAGG
                     NFVDTADVYSNGVSEEITGRALKSVRDDVVLATKVRFPMGEGPNDVGLSRKHIMQGCE
                     DSLRRLDTDYIDLYQVHCWDAVTPVEETLSALTDLVRSGKVRYIGVSNFTGWQLMRSL
                     EVSDAHGFERFVCLQPQYSLVERNIEREILPVCVEEGLGVIPWSPLGGGFLSGKYRRD
                     EEPPDGSRIAGAEESMEEYWDRRATERNWATLDVVGRISEETGKSYAQISLNWLLRQD
                     GVTAPIIGARTLQQLEDNIGACGWELTGEQVAELSEASALEDVYPYRFIRNAQRA"
     gene            9665..10678
                     /locus_tag="VHM69_07840"
     CDS             9665..10678
                     /locus_tag="VHM69_07840"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740342.1"
                     /translation="MRAGGTVNTGDIDWAALPALYVPFPSAINEYADSVHLRTTEWLR
                     RFMVLANETAYRRFSDIKIGRLAARFHPEAPLDALQLISDWYGWMFFWDDQRDETEIG
                     KQPSLLAARNVGFLEVLTDAGSSESDPLARALRDLRERLLAKATSEAWMRRFVGSIEE
                     HFASTAWEATNRSLGVTPDMDSYVRMRPITGGLNIDTQFIEIAEQAYPPPKIWSDDTV
                     RGMVLASNNAVCWANDMFSLEKELRRNDVHNLVLILQLEQGLTLQAAFDRAIEMHNSE
                     VRAFVDSASRLRQLDGPVDDVLRRFVTILQTRMSGFVDWARESGRYHSPVSTGQPRIG
                     GQR"
     gene            10675..>11933
                     /locus_tag="VHM69_07845"
     CDS             10675..>11933
                     /locus_tag="VHM69_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX2740343.1"
                     /translation="MTGRSDSSLQAVQLRQLIGWLTPVAFGFVLLSGVVSVAFGDVRL
                     GIAGVLIFIFGCLVLLARAQVRRGRWQAAVITICVGILGTTLAVAPVQPGWFPTLAVT
                     PFLMVMVALPYTNDHVLRRLLIGAWLVTLLVVIVGQVVPSSPAVPSLVQSVFLVSSLG
                     AAVATVFLLLWQFRSRLTSMLAQTREAEERYALAAQAANDGLWDWNLVTNEVYFSPRW
                     GAMLGYEEREVGSDPGEWFDRVHPDDRAGVEARLKAHLDGQSGNFESEYRVVHKDGGY
                     RWMLVRGIAVRDADGKAVRVAGSQADITERKRAEASLQEAESRYRMLVERMPAVVYIQ
                     EIGSPDSAMYMSPQIEALTGYSPEEVQDLDLRWRIVHPEDRERMQAEDELDIEPGEVS
                     TTEYRVLHRDGHTVWVRNESVLLDDEA"
BASE COUNT         2173 a         3734 c         3887 g         2139 t
ORIGIN      
        1 cccggccacc gcgccgatcg agatcaccgc cgctatcgag gcgacgaacg gctgctcgtg
       61 ccctaacacc agcgccgcag cagaccatgc aagggtcgcc gcaacagccg tctgcacgac
      121 cggccacatg ccggcccgca accgcccgag agctccatct atggcacggc ggatcgccga
      181 aactccgcct cgcctcacaa cggaaagtgc accggaaatc gccatctcac ctgcctccga
      241 acttgctcta cgtatctaag tgcttatagc atataccatg atcaatctag catctctcgc
      301 gcggcaaaca agaggatcgg gcgggttttt gcggggtgtt atcaaactgt ttccatatcg
      361 ttgcagagtg cttccgattt cggcaaacga tgatcaggtc gttgggatgg ttacgccatg
      421 agggccgttc ggcctctccc tcagggtccg gacgtaatct aacccacgcg agggtttacg
      481 caccggtggt acgtcgcagg cgctcgtcga gcatgttgag cactcgttgc caggaatctt
      541 cgttggcgaa ggcgttgccc ttgggatctc ccccgttgta ataggttccc ggggtggctg
      601 gcaggtaggg tggctggatg ccgtgcccgg cgtcggggta gtgaaggaac ccgtcgaggt
      661 agtgtcgctc gaagcgctgg agacggtcca tcgcgacctg cgagagctgg gtcgagggcc
      721 acacctggtc ggcgtcgcct gagatgagga gtacgggtcc gttggtgcgc tcgacgggta
      781 tctcggccct ttcgagctgg tccggcgtcg cctgcagaat atcgaatggg ttggggatcc
      841 agggcagcgg ttcgtcccgg aaggtccacg cgggttcccg actatcgggg gaggggaaga
      901 cgagcccgct tccggaataa ctcacgacgg cccccagttc ggggtagtgc gcgccgagga
      961 gcagggcgag ctcgccgcct cgcgaggtgc ccatcacccc gagcctgtcg cctcgcacgg
     1021 caggctggtc cttcagccac ccgatggcct tcccgaaata ctccagcggg atgcgggcga
     1081 gcctgtccgg cagcgggccg aagtagaaat acgccagcgc gagagcggcg aacccgtggt
     1141 gcgcaagtag tgccgcctcc ctcatcacgt atgaggcgag ccctccatca gagccgccca
     1201 ggacgagcat gccaggggcc ggctcgccgg tcgctggcct gaagaacttt ccgtagagtc
     1261 cgcgggcgcg gacgtcagtg ctctctatat cgctggtaag gaggtgcctg acgacccgga
     1321 cgccggcttt ccggccatca gtctccgctg tgatgaacag gggacgcgga cgcagggaga
     1381 tagcgtacga agtccccatg ccgtctgccg accacacgag gcccatcggg tcagcccccg
     1441 tgtagctgcc tcccagcggg gcctgctctg acacgttgac cgtacccagg aaatccgccc
     1501 ggaacgtggc tgtggaagtc cactcgacat cgtagccgtc gaagaaccgt gcgctcagtg
     1561 tcacccgttg ccatggtgaa aggtccttca ggatgatggt gaacggttcg tcgtccctga
     1621 cggaattggg ggtgacgcga agcttcccgg ccagactgga gtcgtcgcag gcggtaaaca
     1681 atcctccgca agacgtcccc gccacgccca ttgcggcaac ccgcaagaac cctcgtcgcg
     1741 tgtagagccg ccgaggatca ggtgcgtcgc cgttcatagc tcggggatgt ggctccctcc
     1801 ggtgcacctg ccggagatcg ttcacccgtt gtcctcctgg tccagccctc ctcgtagtct
     1861 acaaggttca ggaggcaggc ctatagcttc agtagagcag acgtttgtcc aggacgttga
     1921 gcaactcttc gcctgcgagg taacgacgca ggttctcaca gaaaaggtcg gtctgtgcgg
     1981 ggttgatcag gtccggcacc acgtccgtgg tgtgggcgct gacgatgacg ttatcgaggt
     2041 cccacaacgg gctctcatca ggcaaaggct cgacctcgaa cacgtcgagc gcggcgccgg
     2101 agagatgccc gctctccaat gcctccacga gcgccgcctc ttcgaccaca gtacccctcc
     2161 ccacgttggc gaagtatgcg cccggcttta tggatgccat cgcgtccgcg tccagtagat
     2221 gcctcgtctc aggggtgccc ggcagcgtaa cggccacgta gtcggcctcg ccgagggctt
     2281 tgcgcaggtc agcggtggcg tacagctcgt cggcgtactg ccatgctgca tcgtcctcgc
     2341 gcaccgtgcg cttgaccccg acgacgcgca taccgaaagg ccgcgcgcgc tgtgcgatgg
     2401 cgcgaccgat gctccccgtc ccgacgacgc acagcgtctt gcgttccagg gttcccgtag
     2461 tcccctgccg ccataccctt tcggccttct cgcggcgcag gcggtcgagg cccttggcgt
     2521 gctggagcat cgccatcaga acgaactcgg cgagcggccc cgagtagatc ccactcgccg
     2581 tcgtcacaac gacggcggtg tctccaagac cagccttctg cgccacctcc ccggcgccag
     2641 ccatgctccc ctgtacccac ctgagtttcg gggcgacctc gtgaaggtcg ccaacgtgac
     2701 cccgggggaa gtcgtagatg acctcagcct ccccgagcat cgccagaaac tcctcctcgc
     2761 cttcaggcgt tcgcctccag ccttcgggtc ccgcgtggtc accgggccac cgcggaggcg
     2821 ggaccagatc ctctctatag agtacccgca accgcccgtc cacctcccgt atacgacgga
     2881 tgtgctcttc ctcgaagtaa ctggttatca tgaccgtggt caacacgcct ccttttgtag
     2941 gtttcaggca tcagcttaaa ggttttcctc gatgtctgag tgcctacgga tctcgtgcta
     3001 ctcttcccca tgtggaggag ctgctcgaca tcctggacga gtcaggaagg cggacagggg
     3061 aggtcgttcc caagagcgag gcgcaccgca gggggctctg gcaccggggt ttccactgct
     3121 ggatctgcgg cacggacact actggcgagt cttacctgct gatccagcgc cgttccgcga
     3181 cgaaagacac ctggccgggc cggctggaca taacagcagc aggacatcta accgccgggg
     3241 aggggaccct cgatgggctg cgcgaggtcg aggaggagtt gggcttgagg gtcgagcccg
     3301 agaggctcat cccgctgggg agccgccgcg ccgagcaaga gatacccggt gggtacgacc
     3361 gcgagttcca cgaagtgttc ctgctggtgg ataccactcc cccggagagg atgcggctcc
     3421 aggtcgagga ggtggatgcc ctcatcagga taaggttgga tctcgtggac gcgctcggaa
     3481 atggtgagag cgtccctgcg aaggagtacg cggatgggaa aacggtcgcc acgcgggtat
     3541 acccctcgga cttcgtcccc aacagcgacg actacctgcg cagggtggcc ggtgctgccc
     3601 gccgggtact cgccggagaa gagccgggac gggtcttcta gtcctgcggg tcttcgatct
     3661 gcctgacggc gagatcggca cgccagcccg cgctccgtgc gtctctgtgc gtgttttcgg
     3721 cgtggctctc gtcccgagag ccggttttcg actcggagag ggagatcagg gctcgggtgg
     3781 caccggcggc caggtgaacg gccgccgtcg cgaggggcct gccttcctcc gtgaggtcct
     3841 ggatgttgca ggcggcgagc gtagccaggt ccgagcacgc ctgcgcggct tcgatcaggg
     3901 cgtcggtttc ttctgaatcg gaaagggctt cgaggcggcg cccgaggtcc agcgccagtt
     3961 ccctgaccac ctccatccgc agggagttat tcgggctcgg cccacacgcg tctacgagca
     4021 ggcctgcggc catggctcct gcgtcagcgg gtgtgcactt tctggcgacc atcgcgcggg
     4081 attttatccg aaggtcggct atgccgagta tatgcggcgg taacgagcga tcgcgaacat
     4141 aaaagcgccg tagatgagga gtcctgcggc gatggcgctg agcatgtacg agccgagagg
     4201 ttggtgctgg acggtttcca gcgcggcgcc gaggccgcgt gtctcgctcg gatccgactg
     4261 gtaagcggcc aggacaagga aggccccggc cacgccgatg gcgagggcgc gcgccgcggt
     4321 cccgatgcga ccggcgtaga tgacccagcg ttcctccgct tcgcccatct gtcccagtct
     4381 caggtctgcg cggaacttcg cctcgtaggc ggcgtagagc tggtagagac cgaccccgat
     4441 cacggccatt cccaccagcc cgacaaggat ctggccgagc ggcggctgga aggccatggc
     4501 gctcgccgcc gcggcgtcct ccgagctgtc ttcggcgccg aggatggact gcgccgcggt
     4561 gtaggcgagg gcgccatgga tcgcaccgct ccctacgtag gcgacacgcc gcatgatgcc
     4621 gtgggcgtct gagcctttgc cgtcgggatc cgttatcccc tgcaccacct tccacagcgc
     4681 gtagcacgcc agaccgacgg cgaggagggc gagcatgatt cctccgagcg gctgcgtccc
     4741 tatgctctgc atggcaccgc tcgggtctgt ggtctgtcca cctgcgccaa gagcctcacg
     4801 caaagccaga aacccgacca cggcgtagac gacccccttc gcgacgtacc caagccgcgc
     4861 caaccgctcc acccacgggc tggcgtgcct ggctgcttcc gcggcgtgct ccttgacctc
     4921 ctctcctcgc aactggttgc gcgccacgac cggactcctt cccgcttcga ctacccggaa
     4981 tactctacac gagcgtcggt cggcacgaac ccgcccggcg cgctatatta ggcgcatgag
     5041 ggttgcgctg gcccagatca acaccaccgt cggagatatc tgggggaacg tcgagaagat
     5101 gtccgacacc ctggggcggg cgacggatgc cggcgcggag ttggtcgcct atcccgagct
     5161 gaccattccg ggctatcctc ccgaggacct ccttatgcgc ccgagcttta tcgaggagaa
     5221 cctgagagca ctcgacgagt tcgccgggaa ggtgcccgag ggcgtggtcg ccgccgcggg
     5281 tttcgtggac ctggacgccg acctgtacaa ctcctgcgcc gttctgtccg gcggcgagat
     5341 cctgcaccgt taccacaagc gctacctgcc taactacagc gtcttcgacg agaaccgcta
     5401 cttccgcgag ggttctggag cccccatcgt cgaacttggt ggcgccctcg tcggggtgag
     5461 cgtgtgcgag gatatctggt atccgggcgg gcccgcccgc gaacaggcgc tcggcggggc
     5521 gagtgtgctc ctgaatatct ccgcctcccc ttaccacagg cgcaagggcg ccttcaggga
     5581 acgtatgctc ggcgtgcggg cgtcggacta cggctgctac gtagtctttt gcaacctcgt
     5641 tggcggccag gacgagctcg tgttcgacgg gcactccgtc gtcttcgacc ctgagggcgg
     5701 gctcgtcgcg agagccgacc agttcaaaga ggaccttttg atcgttgacc tttaccccga
     5761 ccactctcta atgcagcgtc tccacgaccc gaggcctcgc aaggagaacg tggagcgtcc
     5821 tccacagata gtgaaggttc cagactacgg gccgcgttcg gtggagcccg tcgaggccag
     5881 ggtcgagcct tacctttcgg aagagggcga agttctcgaa gccctcgtgc tcggcctggg
     5941 ggactacttc cgcaagaacg ggttctcccg cgcggtgctt ggcctatcgg gcggcataga
     6001 ctcttctctg gccgccgccg tggccgtcga ggctctaggt ccccagaacg tcacgggcgt
     6061 cctcatgccg agccgctaca cctccgaggt cagcaacaca gacgccgcgg cgctcgccaa
     6121 gaacttgcgc atagataccc agaccatccc tatcggccct gccttcgacg cctacaggga
     6181 gatgctgaaa agcgccttca agggattgcc cgaagacgtg acggaggaga atttgcagag
     6241 ccgcatcagg ggcaacatcc tgatggccct ctccaacaag ttcggctgga tcgtcctgtc
     6301 tacaggtaac aagagcgaga tgagcgtggg gtactcgacc ctctacggcg actcggcggg
     6361 cgggttcgcg gtcataaagg acgtgccgaa gaacctcgtc tacagggtgt cgcgccactt
     6421 caacgagagg aaggaccggg aggtcatccc tgactccatc ctcaccaagg agccgaccgc
     6481 agagctgcgc gaggaccagc gcgacacgga ctcccttccg ccctacgagg tcctcgaccc
     6541 catcctcgag gcgtacatcg aagaggacaa gggggtggcg gagatcgttg cctcaggctt
     6601 cgacgaggac gacgtacggc gcatcgttca gcttgtggac cgggccgaat acaagcgccg
     6661 acaggcgccg acaggtataa aggtcaccgg ccgcgccttc ggccgtgatc gccgcatgcc
     6721 tataaccaac cgctacttcg agcgcaacgc cgaagaatag ctagatctag acttccgctg
     6781 aacccgtggt gtccggcgca ttcttgtggg tgctgaacct catctgtacc gtggtcccgc
     6841 gtccgggggt ggagatcacg tccagctccg caccgacctc accggcacgc cggcgcatgt
     6901 actggagacc gaggccaggc agcggttctc ccgtgtcgaa tccgcggccg tcgtcgcgta
     6961 cgcggataat gagcaccgag cccacgtgac gggactccag atacaggttg ctcgcgccgg
     7021 agtgcttggc gacgttccag caagcctcga cgaacaccct gatgaaggcg gcgatctcgt
     7081 cctggtctaa cccttcgagg ggagcctgga ggtcgtcgtt ggccttgata ccgaagtact
     7141 cctcgaactt catcagacgc tgacggacga agtcgccggc cctgggccgg ttctcgctgc
     7201 gcagggtctg aagctcatcg taggggcgag agatctggaa ctccgcctcc cttgacaccc
     7261 caagcgccct ctcgaacatc tcatgcgcct cctccctgtc tccgcggcgc tcggtgtcga
     7321 gggcggcgcg cagcgtcagc gagataccgt gcacgttctg ctttaccgta tcgtgcagct
     7381 cgtagagcag gcggctctgc tctttctcac gggccgactc ctcccgctac tggctcaggt
     7441 tcctgctgac gaacgagacc agcccgatac gcgcagccat atacaggagg agcacggcgc
     7501 atcctgtgag cacgtacgca ggcagcggtg ggtttatgac gccaaggagg agaagtatcg
     7561 tgaactgcac cggaggggag agcggaccga gccagaacaa gagtatccgc cacggttgta
     7621 taagcctgac gttcgggtcg gcggggacct cgggtgaggt cgttgaggtg aggccgagca
     7681 ggatcatacc tagggcccac agcatctcgg gccagttgcc gctctggtaa gggcctgcgg
     7741 agcggagccc caggtaagcg gcgtcggcca gaagcagcgc gacgaatccg ccgttcaaga
     7801 acgcggagaa gcggggcctg gcgggagacg aagcgacgac gagcccgagg aagagcaggg
     7861 ccgcgtcgca gaccggctgg gagagggcaa ctacggcgtc acgcacgctc cctggctccg
     7921 ccggaccgag gacgaagtac caggccagcg tcccgacaga gagcatcacg gccccggcgt
     7981 cgatcgcgct gaccagcgtg atcctgcgcg tcgcgagcgc cacgaggtgc agcatcgccc
     8041 cgaccagcaa cgtgtaggaa acggcgtagg cgacgtcttg cggggccacc ggtctcacga
     8101 tgccaagatc gaggagttga gaggtactcc acacagagtt tccagcgagc ctgaagacca
     8161 tgccccatcc catgagccgc aggaaggccc gctcgtggcc ttgtgcacgg ggcgcggccc
     8221 tgagcaggat cacggcggcg aaggtggcgc tggagaggga gaagatcgtc gcgattaccg
     8281 acttcgcatc cgagccttga ggcagagagg cgaagacgag cgcgcccgcc gcccagcaga
     8341 ctagagcgac tgtcgcaggg atgcgcgggg cgcgaggatc gcgcagctta cccgcctctg
     8401 aactttgcac cctttaccct ccagaactcg aaccagccca tcctatcaaa ctcgtaccgt
     8461 gtgtagccag tacggacggc catatcctgc atgttaaagt agaacctttc tacggaggag
     8521 gagcgaatgg agtacaggcg tctcggtaac accgggcttt tggtctccga gctgtgcctg
     8581 gggtgtatga ccttcggaca ggaggctgac gaggagacgt cgaaggagat cgtcggccgc
     8641 tttctagatg cgggcggcaa cttcgttgac actgccgacg tatactcgaa tggcgtctcc
     8701 gaggagatca ctggcagggc gctgaagagt gtccgcgacg acgtcgtcct cgcgacgaag
     8761 gtccgctttc ccatgggaga aggcccgaac gacgtgggcc tctctcgcaa acacattatg
     8821 caggggtgcg aggacagcct caggcgtctg gacacggact acatagacct ctatcaggtg
     8881 cattgctggg atgctgtcac gcccgtcgag gagaccctct cggcactcac ggacctggtc
     8941 aggagcggca aggtacgcta catcggcgtc tcgaacttta caggctggca gctcatgagg
     9001 agcctcgagg tgagcgacgc gcacggcttc gagaggttcg tgtgcctgca gccgcagtac
     9061 tctctcgtgg agagaaatat cgaacgtgaa attctacccg tctgcgtgga ggaggggttg
     9121 ggtgtgatcc cctggagccc gctcggcggc ggcttcctct cagggaagta caggcgcgac
     9181 gaggagccgc ccgatggctc gcgcatcgcc ggggccgagg agtctatgga ggaatactgg
     9241 gaccgccgcg ccaccgaacg caactgggcg acgctcgacg tggtaggcag gatctccgag
     9301 gagaccggaa agtcctacgc ccagatctca ctcaactggc ttctcaggca ggatggcgtg
     9361 accgcgccga ttatcggcgc ccggactctt cagcaactgg aggacaacat cggcgcgtgc
     9421 ggttgggagc tgacgggaga acaggtggcc gagctctccg aagccagcgc cctcgaggac
     9481 gtctatccat accgcttcat ccgcaacgcc cagagggcat aggggcggcg agcgttctag
     9541 ggactggtga tcggggcccg tgtcctggca cggcgcgttt acgctcgttg gcgacgtgtg
     9601 tccggtatag tatcggggtg cgtcttggcg ggcactggtc gttgcggagc aaggtatcac
     9661 gagtatgcgg gccggaggta cggttaacac gggggacatt gactgggcgg ccttgcctgc
     9721 gctttacgta ccgtttcctt ctgcgatcaa cgagtacgcc gactccgtcc atctgaggac
     9781 aacagaatgg ttgcgtcgct tcatggtcct tgcaaacgaa acagcgtaca ggcggttctc
     9841 cgacataaag atcggacgtc tggcggccag gttccacccc gaagctcctc tcgatgctct
     9901 gcagctcatc tccgactggt acggttggat gttcttctgg gacgatcagc gcgacgagac
     9961 ggagatcggg aaacaaccga gcctgctggc ggccaggaac gtcggcttcc tcgaggtgct
    10021 gacggacgcg gggagctcgg agagcgatcc cctcgcccgt gccctccggg acctgcggga
    10081 gcgcttgctc gcgaaggcaa cctcagaagc gtggatgcgc aggttcgtcg gcagcatcga
    10141 ggagcatttc gcttcgaccg cctgggaggc gaccaaccgt tctctgggcg tcacgcctga
    10201 tatggacagc tatgtcagga tgcggccaat aacgggagga ttgaacatcg acacccagtt
    10261 tatcgagatc gccgagcaag cctatccgcc gcccaaaatc tggagtgacg atacggttcg
    10321 cgggatggtc ctggcgtcga acaacgcggt ttgctgggcc aacgacatgt tctccctcga
    10381 aaaagagctc aggaggaacg acgtacacaa ccttgttctg atcttgcagc tcgaacaggg
    10441 tcttacactg caagcggcct ttgaccgtgc catcgagatg cacaactcag aggtaagggc
    10501 ctttgtagac tcggcctcgc ggcttcggca actcgatggg ccggtggatg acgtactgag
    10561 gcggttcgtt acgatcctgc agacgaggat gagcgggttt gtagattggg cgcgcgaatc
    10621 gggacggtac cattccccgg tgagcacggg tcagccgcgg atcgggggcc aacgttgacc
    10681 gggcggtcgg actcctcctt acaggccgtc cagttgcggc agctcatcgg ctggctcacg
    10741 ccggtggctt tcggcttcgt cctcctgtcc ggcgttgttt ccgtcgcttt cggcgacgtg
    10801 aggctcggga tcgctggagt cctcatattt atcttcggat gcctggtcct cctggccagg
    10861 gcacaggtcc gccgtggccg gtggcaggcg gcggtcatca ccatctgtgt aggcatcctg
    10921 gggacgacgc tggccgttgc gccggtgcag cctggctggt tccccaccct cgccgtaacc
    10981 ccgttcctga tggtgatggt agcgctccct tacaccaacg atcacgtcct caggcggctg
    11041 ctgatcgggg cgtggttggt gacgttgctg gtggttatcg tggggcaggt ggttccctct
    11101 tcgcccgccg tgccgtcgtt ggtccagagc gtattcctgg taagctcgct cggtgctgcg
    11161 gtcgccacgg tgttcttgtt gctgtggcaa ttcagaagcc ggctcacaag catgctcgcc
    11221 cagactcgcg aggcagaaga gcgttacgcg ctcgcggccc aggccgccaa cgacgggttg
    11281 tgggactgga acctcgtcac taatgaggtg tacttctcgc cgcgctgggg ggcgatgctc
    11341 ggctacgaag agagggaagt cggaagcgat cccggagaat ggttcgaccg cgtgcatcct
    11401 gacgaccggg ctggcgtcga ggcgcgtctg aaggcgcatc tggacgggca atccggaaac
    11461 ttcgagagcg agtaccgggt cgtccacaaa gacggcggtt atcgctggat gctggtacgt
    11521 ggcatagccg tgcgagatgc ggatggaaag gccgttcgcg tcgccggatc ccaggccgac
    11581 atcaccgagc gtaagcgcgc ggaggcgagc cttcaggagg ccgagtccag gtaccgtatg
    11641 cttgtcgagc ggatgcccgc cgtcgtctac atccaggaga taggcagccc cgattcagcg
    11701 atgtacatga gcccccagat agaggccctg acgggttact ccccagagga ggtccaggac
    11761 ctggatctca ggtggcggat agtacaccct gaagaccgcg aacgaatgca agcagaggac
    11821 gagctagata tcgaaccggg tgaagtatcg acgacggagt atcgggtact tcatcgcgac
    11881 gggcatacag tgtgggtgcg taacgaatcg gtcctgctcg atgacgaggc gag
//
LOCUS       DATAIR010000320        10915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20365, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000320 DATAIR010000000
VERSION     DATAIR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20365"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..400)
                     /locus_tag="VHM69_07850"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="VHM69_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
     gene            complement(601..1905)
                     /locus_tag="VHM69_07855"
     CDS             complement(601..1905)
                     /locus_tag="VHM69_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740344.1"
                     /translation="MSPRASPATLSGRWLLIARMAWVAVAIVALAIVLFSIPSSFEYY
                     RSVCTAASEVCSEQAVGQPTPVGMEALRDVGLSASSYALLRVVIDKVFQLVWFAVGAL
                     IFWRRSDDWMALLVSALLVSFGPVSVDTTDAYALISSQPAWLLPVRSVEIVGIVCSAL
                     FFFLFPGGRFAPRWTRWLAVAFIAFQSSGTFFAGLYSSSPALETVSYLVFLGMVVSLV
                     WSQVYRYRRVSSPAQRQQTKWVVFGTTLGVAGTFPLQLPVDLSLVDGDTPLTLLFLKA
                     GFALSFMLIPLSIGVAVLRSHLFDIDVVINRTLVYGSLTALLVALYFSGILALQRGLV
                     VLTGETSTLAVVASTLAIAALFNPLRHRIQSFIDRRFYRRKYDARKTLETFAAALRNE
                     TDLDELSDNLTRVVTETMQPAHASLWLRPEGPGGERNPVNER"
     gene            complement(2138..2584)
                     /locus_tag="VHM69_07860"
     CDS             complement(2138..2584)
                     /locus_tag="VHM69_07860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2740345.1"
                     /translation="MSDDVGTLNQEQLTRLARYFDEKVTFSRHIKSKVEEVEPGKAVC
                     YIDVEDIHLNGNGTLHGGVYASLIDNAMGLAVAALVGLRTATIGLDVKFLGAVREGRI
                     SCSAEVVHRTRRIATVEARVRNGGDNLVALGTGTFRIFEKRGDPIV"
     gene            complement(2681..4567)
                     /locus_tag="VHM69_07865"
     CDS             complement(2681..4567)
                     /locus_tag="VHM69_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740346.1"
                     /translation="MSRLRRVRITRTLPAHAVAASFGRSALATFSDGMRKLVLADLQD
                     GVVRVGDLPRLTRNLVWLGFVLVFAGVGVLFFNEPLRAVFPLLPMIGGTTGRGELVPF
                     TLIPLTLFILSMAWGFMLAGALHARLVVRLAVLLLYLVVAGVWTASTSSNILASLVAW
                     SALLFVPVFFFLRRQAKEAPAFEFGVLLVSISLTFGISQVYAIEAWRISGVPGVLANV
                     PTMLYTFDLLVIPLLLFIGVDIAEFVHRTASWTSGLTEAWTSRMVLYGALFALLVWRL
                     VSVMLETADRVGRSSPAEQALSYAGALVIPLSVGTVWWLIRRHDGEGVSVERLVDVAK
                     RVAMPLIGAYMGLQAVEIVLTLVAGLVGLLFLGLGFGKIEQLGAALEFIGRLIGQDAN
                     WQTLLAVLTILATIWFARRGRRTLALYLGILGTDALWIQLTNPGRLLSFLGWSGLEPV
                     DFWWVVIFSAVAFFWVVRGRLTEDRARRLLMLVLITLLMRQTGFIEDPFSPFLGFTGV
                     GMIALGIVWDALTIGFWANNDSPGMPRVSRILLYLGYVLFSIAVINWSVASHDISQVD
                     FFTGQAALGGFSLFGKPLIYAIFAVTLAESVVDGRDDEPAATGQEERDLDTRGTWRPS
                     KPVR"
     gene            complement(4564..7416)
                     /locus_tag="VHM69_07870"
     CDS             complement(4564..7416)
                     /locus_tag="VHM69_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX2740347.1"
                     /translation="MLVLAMAFFFAFPWLSEPSLGGAALGRYSPARDGASLLVESYDK
                     GGRLISTESQNLAMIPALRSFTESRQGIREELEEIYGSPQAMDDARVMEVRRRTVKES
                     GEVSESTDTFVLDPRGLFLLGTREGAGGTDIVFDDPALMLPADLGPEKSWSSEGRAGS
                     LDYEMDGRVVGTTTFRGDLGSFDDCLSVEMSLILSPTGSPDVLTTYKDVYCAGVGLVE
                     SKEFDGSGGLTRRSTVVSTDQTPGVREARLQPASVAPREEISGDPAKWRLRHFGRLMP
                     TGESTAGTIPPTYVPTDPPVVLAAALEGDLLALDAGKSPGTVRWRFHPEGTVYGPPAF
                     DAETGRVYFGATDKKLYALDARGLFLWAYETGDNVASRPVVVGETVVFGGETRNVYGL
                     DARTGEERWKVETGGPVVSSPDLSNGLVVIGSDDSAVYGLDPSTGEQKWLYLAEGPVE
                     APVTAAGGVVYVASRSGEITALDAKTGKEIWASAPGDVLRTAPAVSETSVFVVDESNR
                     LLAYDRRTGKKRWDIPNGSFVGPPLVAGEGLLVARSDGRIQRVDLDGEIREEWKGSEV
                     GNPIDGDPAFSMGPTAGGGAVWANADTSAVLRLGPAAGPAPIEPVWAAAFANPPFSGD
                     ALQYTAAGYRDEALLLGASNNIYLLDVQSGEARRVGGLDSASGPVSEPVVAEDILLAA
                     SGEGLHAVQLPDGKAVWNIGGGTGLRPPAVAGETVLWLSTQEDGGSQALKALDLATGK
                     VLWEAPLSSAGGVIVRGQTAYANPAAAFDIETGRPLWRAETGGSSSGGPALSESGDVL
                     FAGTSGGGPGSVVAVGTRGGEELWRAGLEGESVNPSDRLWVSGDSLVVPALSGAVIAL
                     DIATGEQLWRYEPPFPRLGNVTVEGGRVWFALQNGEILALDSGSGEVVARSNDYSLNL
                     SNASIAQRPSFVGGKLVLGVGTYVLGFESPGEAGGG"
     gene            complement(7491..8111)
                     /locus_tag="VHM69_07875"
     CDS             complement(7491..8111)
                     /locus_tag="VHM69_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740348.1"
                     /translation="MAAAQVTAGGAYALSLAFVVAILYLALVRFMDLNEKEPLWAVGL
                     MFLLGAVTAAVLPLLVGYAVLELNPLLGAVSEEVAKFVAFFIGVVVLATLWTTALVGL
                     SDGLFGVIIGAGFGAAAGARPMESGLISQQQYDALGSVVGRMGSTFRTLRSGGFGGWR
                     ANARFSQAATELAFHRDRVQRGVTSVDAASRESAYVQTLRELRART"
     gene            complement(8304..8720)
                     /locus_tag="VHM69_07880"
     CDS             complement(8304..8720)
                     /locus_tag="VHM69_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2740349.1"
                     /translation="MSGAPRWSVGHVIEEQTLPPVDRMRLIKYAGASGDFNPIHLLDQ
                     AAADAGLKGIIAHGMLTVATIGLLFSPYLEHGYVKTFRSRFSGMVFLGDVLHVGGRVS
                     GVEETDDGRLYAFDVYARREEDTVASGEVGFLVYED"
     gene            complement(8717..9163)
                     /locus_tag="VHM69_07885"
     CDS             complement(8717..9163)
                     /locus_tag="VHM69_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2740350.1"
                     /translation="MLYAEVVGSRSEPVRNLVERGAVRRFAEAIADPNPLYVDETVAE
                     QSRYGALLAPPTFPRTFDYGRIEGLELPSAGLIHGEFRISYERPLLVGEELLCRMELK
                     DSYDKQGRRGLLGFLVFERTGEDRYGERVFTTNDVVVVTDAVRRGI"
     gene            complement(9163..9924)
                     /locus_tag="VHM69_07890"
     CDS             complement(9163..9924)
                     /locus_tag="VHM69_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740351.1"
                     /translation="MGRLDGRVAIVTGASRGIGEATARRFAREGARVCLTDVNMEGVL
                     STARRLVDEGMDAFAAKVDVTSRTEVENAVGETVDRYGRLDILVNNAGVTRDSLIYKM
                     TDEDWRTVLNIHLSGAFFCTRAAQKHMVERNYGRIVNVSSTSALGNRGQSNYSAAKAG
                     IQGFTKSLAVELGRYGVTVNAVAPGFIETDMTRDTAARLGVDFDDFVAERVQSIPVGR
                     AGWPEDVAAAILYFASEEAAFVSGQVLYVAGGPRD"
     gene            complement(9996..10847)
                     /locus_tag="VHM69_07895"
     CDS             complement(9996..10847)
                     /locus_tag="VHM69_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740352.1"
                     /translation="MDQNTIGVLGTGTMGAGIVQLAAQSGYSVVACDASVEALERAQV
                     YVREGLSRFANKGAFSEEEAASHYDRIHWTTRLEDLGEARISIEAIVEKVGPKKDALA
                     ALDSLLRPDSLILTNTSSISITDLASATGRPESVCGMHFFTPPPVREAVEIPRGLLTS
                     DETVEQTKALVVSFGKLPIVVEKDVPGFVANRFLMPMMLEACRLLEQGVATKEDIDLL
                     VKKGVGFPIGPFELSDFIGLDVGLDVISYVHDRTGDPYYEPPRILKELVRMGRLGRKT
                     GGGFYDY"
BASE COUNT         2102 a         3539 c         3372 g         1902 t
ORIGIN      
        1 gaagatcctc gcgcgtagcc cagtgctcga ccagcttgcc gtccgcgaca cggaaccagt
       61 ggctctggcg agtcaggaag cgcttccctg tcggtggcac gacgccgatg agcggtccga
      121 ggttcgtgcc ctccgagagt acccggacgg ccacggtatc ctcctacgcg acgatcgctt
      181 cgatcgtcat gtgcagatca gggaactgtg caaacagcca ttccgcggtt gcgcgcatgg
      241 cccggggccc gttgactctc ccgggagcgg actgcgcgaa gggcgcgact gcgtgctcga
      301 cgaaatcttc agccatgatc tcgtcacagg ccgccaggtt cttgtggttg aagatctcct
      361 cgaagtgacg ccgaaccagc tccttgcttt cctccgacat ggttcctccc tcagatagtc
      421 ggtagcagtc aggttctcga gaatcgctgg ctgacttgag gccgcactcc tcctgcgctg
      481 cagatcggcg tcctcgcgtg aactatggcg accatacctc agaggtgggt gcaagatgac
      541 agaggactcg ctagagagcg ctgccaagat aagtgttgtt gtgggccgtt ggcaatgagc
      601 ttacctttca ttgacggggt tcctttcgcc tccagggcct tcgggacgca accacagcga
      661 ggcgtgggca ggctgcatgg tctcggtcac cacgcgagtc aagttgtcgc tcaattcgtc
      721 gaggtctgtc tcgttcctga gcgcggcggc gaacgtttca agcgtctttc tggcgtcgta
      781 ttttcgtctg tagaagcggc ggtctataaa cgactggatg cgatgtctca gtggattgaa
      841 cagcgcggcg atggctagcg tcgaggctac aactgctagg gtggacgtct cgcccgtgag
      901 gacgaccaag cctctttgca acgccaggat cccgctgaag tagagtgcca cgaggagagc
      961 cgtcagggaa ccgtagacga gggtgcggtt tattaccacg tctatatcga acaggtgcga
     1021 gcgcaacacg gctacgccga tggacagcgg gatgagcata aaggacagtg caaagcccgc
     1081 tttgagaaac agtagcgtga ggggcgtgtc cccgtcgacc aacgagaggt ccaccggcag
     1141 ttgaaggggg aaggttcctg cgacacccag ggtcgtgccg aacacaaccc acttggtctg
     1201 ctgacgttgt gctggagagg agaccctacg gtaacgatag acctgggacc agaccaggct
     1261 cactaccatc cccaggaaaa ccaagtacga aacggtctcc agggcgggcg aactagagta
     1321 caaccccgca aagaaagtcc cggacgactg aaaagctatg aacgcgacgg ccagccagcg
     1381 tgtccagcgc ggtgcaaatc gtcccccggg aaagaggaag aagaacaggg cgctgcatac
     1441 aatcccaacg atttccacgc tccggacggg taacaaccag gctggctggg aggagatcaa
     1501 ggcgtatgca tccgtagtgt cgaccgaaac cggcccgaag gacaccagta acgccgagac
     1561 cagcagcgcc atccagtcgt cggagcgccg ccagaagatg agcgcgccca ccgcgaacca
     1621 cacaagctgg aagaccttgt cgatgacgac cctcagcagc gcgtaggagc tcgcagacag
     1681 cccgacgtcg cgtaacgctt ccatgcccac gggggtgggc tgccctactg cctgctcgga
     1741 acagacttcc gaggcggcgg tgcacacgct acggtagtac tcgaagctgc tcgggatgct
     1801 gaagagcacg attgcaagcg ccacgattgc cacggcaacc cacgccatgc gggcaatgag
     1861 gagccagcgc ccgctcaagg tagcgggcga ggcacggggg ctcacctcac cgccccctcg
     1921 actcctttta gtttcccctt ctatcacgcc aaccactcta cagggctacc gtctcccagg
     1981 cgttacgaaa cgcgttacgg gagagtgcaa ccacaaaggt gtggaatatt gggagcttcg
     2041 caggagcgct gaaggtagct gcaagaccta cactcagggg ccaggtgacc agcctgaagt
     2101 atctgacctc tccccgttgg cgagttcgtc tttaatccta gactattgga tctccgcgct
     2161 tctcgaagat gcggaaggtc cccgtgccca gcgccacgag gttgtccccg ccatttcgga
     2221 ctctggcctc gacggtggcg atacggcgcg tcctgtggac gacctcggcg gagcaggaga
     2281 tcctaccctc cctcacggcc ccgaggaatt tgacgtcgag cccgatggtg gccgtcctca
     2341 ggccaacgag cgcggccacg gcgagcccca tggcgttgtc tattagcgag gcgtagaccc
     2401 cgccgtgcag cgtaccgttg ccgttgaggt ggatgtcctc gacgtcgatg tagcagacgg
     2461 ctttcccagg ctcgacctcc tcgaccttgc tcttgatgtg acgcgagaaa gtcaccttct
     2521 cgtcgaagta ccgtgcgaga cgggtgagct gctcctggtt cagggtcccc acatcgtcgc
     2581 tcatagaacg ctcccttctg cctggtccgg cggctgcata cagaccaccg acgtatcgta
     2641 ttgctcaacg acgccgtata catcttactt cgccacgact ttaccttact ggcttcgagg
     2701 gcctccaggt ccctcgggtg tcgaggtctc gctcttcctg acctgtcgcc gcgggctcgt
     2761 cgtctctgcc gtccaccacc gactccgcga gggtaacggc gaagatcgcg tagatgaggg
     2821 gtttcccgaa aaggctgaac ccgccgaggg cggcctgtcc ggtgaagaag tccacctggg
     2881 agatatcgtg ggaggccacg gaccagttga tgacggcgat gctgaacagc acgtaaccga
     2941 gatagagcag gatgcggctc acccgtggca ttcccgggga gtcgttgttg gcccagaagc
     3001 cgatcgtgag tgcgtcccat actatgccga gggcgatcat accaaccccg gtgaacccta
     3061 gaaacgggct gaacgggtcc tcgataaacc ctgtctgtcg catgaggagt gtgatcagga
     3121 cgagcatcaa gagccgtctt gccctgtcct ctgttagcct gccccgcacg acccagaaga
     3181 aggcgaccgc gctgaagatc acgacccacc agaagtccac cggttcgagc ccgctccagc
     3241 cgaggaagct caagagcctg ccggggttgg tgagctgaat ccagagcgcg tcggtcccga
     3301 ggatgccgag gtagagggcc agcgtccgcc gtcctctgcg cgcgaaccag atagtcgcga
     3361 ggatggtcag gacggcaagg agcgtctgcc agttggcgtc ctggccgatc aggcgtccta
     3421 tgaactccag cgccgctcca agttgctcta tcttcccgaa gccgagcccc aggaaaagga
     3481 ggcccacaag tccggccacc aaggtgagaa cgatctcgac ggcctgcaat cccatgtagg
     3541 cccctattag cggcatcgcg accctcttcg caacgtcaac gagccgctcc acagagaccc
     3601 cttcgccgtc atggcgccgg atgagccacc agacggtccc cacggagagg gggatgacga
     3661 gggcccccgc ataggagagc gcttgctccg cgggcgagct cctcccgacc ctgtcggcgg
     3721 tctcaagcat cacggagacg agcctccaga ccaggagagc gaacagggca ccgtacagca
     3781 ccatacggct cgtccaggcc tcggtcaggc ccgatgtcca gcttgccgtc ctgtggacga
     3841 actcggcgat gtccaccccg ataaacaaca gcagcgggat gacaagcaga tcgaaggtgt
     3901 agagcattgt cggcacgttt gccaggacgc ctggtacgcc gctgatgcgc caggcctcga
     3961 tcgcgtagac ctgggagatt ccgaatgtta gcgagatgga tacgaggagc accccgaact
     4021 caaacgccgg agcttccttc gcctgccgcc gcaggaagaa gaagacgggc acgaagagca
     4081 gcgcggacca ggccacgagc gacgcgagaa tattggacga ggtgctcgca gtccagacgc
     4141 cggccaccac aaggtacagc agaagaacgg cgagcctgac tacgagcctg gcgtggaggg
     4201 cgcccgccag catgaagccc cacgccatgg agaggataaa gagcgtgagg ggtatgaggg
     4261 tgaaaggaac caactccccg cgtcctgtcg taccgccgat catgggcaga agtgggaaca
     4321 cagcacgcaa gggctcgttg aagaacaaca cgccgacacc ggcgaagacg agcacgaacc
     4381 cgagccagac caggttgcgt gtaaggcgag gaaggtcacc gacgcgcacc acgccatctt
     4441 gcaggtccgc cagcacaagc ttcctcatcc catccgagaa cgtcgccagc gccgaccggc
     4501 cgaaggaggc cgcgacggcg tgcgcgggca gggtgcgtgt gatccgcacc ctccgaagcc
     4561 ggctcaccct ccgcccgcct ctccgggcga ttcgaaaccg agcacgtacg tgcccacccc
     4621 gagcaccagc ttacccccga cgaacgacgg acgctgggcg atgctggcgt tgctcaggtt
     4681 caggctgtaa tcgttggaac gggccacgac ctccccactc ccggagtcga gggcaaggat
     4741 ttccccgttc tggagggcga accacacgcg tcctccttcc accgtgacgt tcccaagccg
     4801 gggaaacggc ggttcgtatc tccagagctg ctcgcccgta gcgatatcga gcgcgatcac
     4861 agcgccggag agcgcgggga cgacgagtga atcaccgctg acccacaggc gatccgaagg
     4921 gttaacgctc tcgccttcga gcccggcccg ccagagctcc tcaccacccc gtgtgccgac
     4981 ggcgaccacg gaaccgggcc caccaccgct cgttcctgcg aagaggacgt ccccagactc
     5041 gctcagcgca ggtcccccgc ttgaggagcc gccggtttcc gcgcgccaga ggggtctgcc
     5101 ggtctcgatg tcgaaagccg ccgcggggtt ggcgtaggcg gtctgtcccc tgacgatcac
     5161 ccctcccgcg ctggagagcg gggcctccca gagcaccttg ccggtggcga gatccagggc
     5221 cttgagggcc tgtgatcccc catcctcctg cgtggagagc cagagcaccg tctcccctgc
     5281 gacggcgggc gggcgaaggc ccgtccctcc cccgatgttc cagaccgcct ttccatccgg
     5341 cagctgaacc gcgtggaggc cctctcctga ggccgcgagc aggatatctt cggccaccac
     5401 aggctcagag acgggacccg aggcggagtc cagcccgccc acccttcgcg cctccccgct
     5461 ttggacatcg agcaggtata tgttgttgct ggcgccgagc aagagcgcct cgtccctgta
     5521 gccggccgcg gtgtactgta gtgcgtctcc tgagaacgga gggttggcga aggctgcagc
     5581 ccataccggt tcaatgggcg cgggaccagc tgcggggccc agacggagta cggcggacgt
     5641 atccgcgttg gcccagaccg ctccgcctcc tgctgtcggt cccatggaga aagcagggtc
     5701 tccgtcgata ggatttccaa cctcagagcc cttccactct tccctgatct caccgtcgag
     5761 gtcaacacgc tggatgcggc catcggagcg tgcaaccagg agcccttcgc cggcgacgag
     5821 cggcgggccg acgaaagagc cgttcgggat atcccagcgc ttcttgcccg ttctgcggtc
     5881 gtaggccaga agtctattgg actcatcgac gacgaagacc gacgtctcgc tcacggccgg
     5941 cgcggtgcgc aggacatctc cgggggcgga ggcccagatc tccttgcccg tcttcgcatc
     6001 caacgcggtg atctccccgg aacggctcgc gacgtaaacc accccgccgg ccgcagtgac
     6061 cggggcctcg acaggacctt cggccaggta gagccacttc tgttctcctg tcgagggatc
     6121 caggccgtac accgcgctgt cgtccgagcc gataacgacc aacccgttcg agagatccgg
     6181 ggaggagacg acgggaccac ccgtctccac cttccagcgt tcctcccccg ttctcgcgtc
     6241 gagaccgtag acgttcctgg tctcaccgcc gaagacgacc gtctcaccca cgacgacggg
     6301 acggctcgcc acgttgtccc ccgtctcgta agcccagaga aagagcccgc gggcgtcgag
     6361 ggcgtagagc ttcttgtccg tcgcgccgaa gtagacccta cccgtctcag cgtcgaaggc
     6421 cggtggccca tagacggtgc cttcagggtg gaaccgccag cgtacggtac cagggctctt
     6481 ccccgcatcg agggcaagga ggtctccctc aagggcggcg gcgagcacaa cgggagggtc
     6541 cgtgggcacg taggtcgggg ggatggtgcc cgccgtcgac tcgcctgtcg gcatgagacg
     6601 cccgaagtgc ctgagccgcc acttcgcagg gtcgcctgaa atctcctctc ggggcgcaac
     6661 agaggcgggc tgtaacctgg cttcgcgcac tccaggcgtt tggtcagtcg agacgacagt
     6721 gctgcgtctg gtcagcccgc ccgatccgtc gaactctttg gactcgacga gcccgacacc
     6781 ggcacagtac acgtctttat aggtagtgag gacatccggg ctccctgttg gagagaggat
     6841 caagctcatc tccacggaga ggcagtcgtc gaagcttccc aggtcccctc tgaaagtggt
     6901 ggtgcccacc accctgccgt ccatctcgta atccagggaa cccgcccttc cctccgagct
     6961 ccacgacttc tcgggcccga gatccgcggg cagcataagt gcgggatcgt cgaagacgat
     7021 gtcagtgcca ccggcgcctt cgcgggttcc gagcagaaag aggccgcgag gatccaggac
     7081 aaaggtgtcc gtcgactcag agacctcgcc cgactccttc accgtgcgcc ggcgaacctc
     7141 cattaccctg gcatcatcca tagcctgcgg agaaccgtag atctcttcga gctcctccct
     7201 gatgccctgt cgcgactccg tgaacgatct gagggccggg atcatcgcca ggttctggct
     7261 ctccgtggag atgagcctgc cgcctttgtc gtagctttca acgaggagag aagcaccgtc
     7321 gcgcgcgggt gagtaacgcc ctagcgccgc gccgcccaga gagggttcgg acagccaggg
     7381 gaacgcgaag aagaacgcca ttgcaagcac gagcacgggt actgctccga tcctgaccgg
     7441 ctcggaagag cccagacggc gccacgctcc cctccgcaaa ggataggctt tcaggtcctc
     7501 gctcgaagct cacgcagggt ctgcacatac gcggactcgc ggcttgcggc gtcaacggag
     7561 gtaacgcccc tctgtacacg gtcacggtgg aacgccagct cggtagcggc ctggctgaaa
     7621 cgcgcattgg cacgccatcc accgaagcct ccgctcctca gagtgcggaa cgtagatccc
     7681 atgcggccaa cgacgctgcc gagcgcgtcg tactgctgtt gggatatgag gccactctcc
     7741 atcggacggg ctcccgccgc cgcgccgaag ccagcgccga tgatgacgcc gaagaggccg
     7801 tcggagagac cgaccagggc tgtcgtccaa agcgtcgcga gcaccacgac gccgatgaag
     7861 aacgccacga acttggccac ttcttcggac acggccccaa gtagcgggtt gagttccagg
     7921 acggcgtagc ccaccagaag gggcaacacc gccgccgtca cggctcccag aaggaacatc
     7981 aacccaacgg cccaaagggg ttctttctcg ttgagatcca tgaaccgtac aagggccaga
     8041 taaagtatcg cgacaacaaa tgccagagat agcgcgtatg ctccccccgc agtcacttgg
     8101 gctgctgcca tagtgtctcc cttcacctcg tcagcaattc gcgcacgccg gtatcgcttt
     8161 agcatgcctt ttactcagta ctttggcgat ggaggagcgt cgatgcatcc cccatatgga
     8221 ctattttggt agctttctct ggtttcggga aggtattctc acgaccgatt tgtcacgagc
     8281 ctcacgctcg cacgatcctg tgtctaatct tcgtagacga gaaacccgac ctcgcccgag
     8341 gcgacggtgt cctcttcccg ccgcgcgtac acgtcgaaag cgtaaagtcg cccgtcgtcg
     8401 gtctcttcga cgccgcttac gcgcccgccg acgtgcagga cgtcaccgag gaataccatc
     8461 ccggagaagc ggctgcggaa ggtctttacg tagccgtgct ccaggtaagg agagaaaagg
     8521 aggccgatcg ttgcgaccgt cagcatgccg tgcgcgatga tccccttcag tccagcatca
     8581 gcggcggcct ggtctaggag gtggatcggg ttgaagtccc cggaagcccc cgcgtacttg
     8641 atgagccgca tcctgtccac gggaggtagc gtctgctcct ctatcacatg ccctacgctc
     8701 cagcgcggag ccccgctcat atcccgcgcc tcactgcgtc cgttacgacg acgacgtcgt
     8761 tggtcgtgaa gacgcgctcg ccgtagcggt cctcgcccgt gcgctcgaag acgaggaagc
     8821 cgaggaggcc gcggcgcccc tgtttgtcgt aggagtcctt gagctccatg cggcacagaa
     8881 gctcctcgcc gacgagtagc ggtcgctcgt aggagatgcg gaactcgccg tggatcaatc
     8941 ccgcagaagg cagctccagc ccctcgatgc gcccgtagtc gaaggtcctc gggaaggtcg
     9001 gcggcgcgag gagagcgccg tagcggctct gctccgccac cgtctcgtcg acgtagagcg
     9061 ggttcgggtc ggctatggct tccgcgaagc ggcggacggc gccgcgctcg acgaggtttc
     9121 tcactggctc ggagcgtgat cccacgacct cggcgtagag cactagtccc tcggacctcc
     9181 tgcgacgtag agcacctgcc cactgacgaa cgcggcctct tcggaagcga agtacaggat
     9241 cgccgcggca acgtcctctg gccagcccgc gcgtccgacg gggatgctct gtacacgctc
     9301 ggcgacgaag tcgtcgaagt ccacgccgag gcgggccgcg gtgtcgcgtg tcatgtccgt
     9361 ctctatgaag ccgggggcga cggcgttcac cgtgacgccg tagcgtccca gttcgacggc
     9421 gaggctcttg gtgaagccct ggatgccggc cttcgccgca gagtagttcg actggccgcg
     9481 gttgccgagg gcgctcgtcg acgagacgtt gacgatcctg ccgtagttcc tctcaaccat
     9541 gtgcttctgg gccgccctgg tgcagaagaa ggcaccggac aaatgaatat tcaagacggt
     9601 gcgccagtcc tcgtcggtca tcttgtagat gaggctatcc ctcgtaaccc cggcgttgtt
     9661 gaccagtatg tccagacgac cgtagcggtc tactgtctcc ccgacggcgt tctcgacctc
     9721 ggtgcggctc gttacgtcca cctttgccgc gaaggcgtcc atgccctcgt ctacgagccg
     9781 gcgcgccgtc gagagcaccc cctccatgtt cacgtccgtc aggcacaccc gcgcgccctc
     9841 gcgggcgaaa cgccgcgccg tggcctcgcc aataccacgc gaagcccctg tcacaatcgc
     9901 aaccctgccg tcgagtcggc ccaatgttgc tcctttcagt cgctcaggca tcaggtttca
     9961 ggtatcaggc ttcagttact tgttcttcct gatgcctagt aatcgtagaa ccctccgccg
    10021 gtcttgcggc caagcctgcc catcctgacc agttccttca ggatacgtgg aggctcgtag
    10081 taagggtctc cggtcctgtc gtggacgtag gagatcacgt cgaggccgac gtcgaggccg
    10141 atgaagtcac tcagctcgaa ggggcctatg ggaaatccca cgcctttttt taccagaagg
    10201 tcgatgtcct ctttggtcgc cacgccctgc tccagaagcc tgcatgcttc cagcatcata
    10261 ggcatgagga agcggttcgc cacgaaaccg gggacgtctt tctcgacgac gatggggagc
    10321 ttgccgaagg agacgacgag agccttcgtc tgctcgacgg tttcgtcgct cgtgaggagg
    10381 ccacgcggta tctcgacggc ctcgcgcacc ggcggcgggg tgaagaagtg catcccgcag
    10441 acactctcgg ggcggcctgt ggccgaggca aggtcggtaa tggagatgga cgatgtgttt
    10501 gtgagtatga gcgagtcagg tcgtagcaga gaatccaggg ctgcgagggc atccttcttg
    10561 ggcccgactt tctcgacgat ggcctctatg gagatccggg cttcaccgag gtcttccaat
    10621 ctcgtggtcc agtggatgcg gtcgtaatgg gaggcggcct cttcttcgga gaaggcgccc
    10681 ttattcgcga agcgcgaaag tccctctcgg acgtagacct gggccctttc gagggcttcg
    10741 accgaggcgt cgcaggcgac cacactgtag cctgactggg ccgcgagctg tacgatgccc
    10801 gcgcccatcg tgccggtccc gaggacgcct atggtgttct ggtccactag acttctcccg
    10861 tgcgggagag gttttcggcg tgcaggatca gggtcctgac cctctcaccg aaggt
//
LOCUS       DATAIR010000321         4355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_332181, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000321 DATAIR010000000
VERSION     DATAIR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332181"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VHM69_07900"
     CDS             <1..330
                     /locus_tag="VHM69_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740353.1"
                     /translation="LIELLVVVVIIGILTSIAIPTYVGQQDEAKDAAAGAQLRTAATS
                     QQLYYARHDAYASDAADLEAFGFRQGEQVVTVGTASASNYCMQAQGGSGTFNITEDSG
                     RPESGSC"
     gene            complement(544..765)
                     /locus_tag="VHM69_07905"
     CDS             complement(544..765)
                     /locus_tag="VHM69_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740354.1"
                     /translation="MIRERATGRVEVRREDPAYRSGWEDGRFGQTQTFVANNNLAAWT
                     EYPDRLSYYRGHRDGRRIRDMLSSSGRSA"
     gene            complement(924..1667)
                     /locus_tag="VHM69_07910"
     CDS             complement(924..1667)
                     /locus_tag="VHM69_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX2740355.1"
                     /translation="MLTQLRRATPRPLILACALSFVAAYFAARFPGVPGASTASYVST
                     LLIALPSTVALFRYLGPRRATLSLLVLSVFACAVEAIGVATGFPYGPFHYGDALGPRI
                     AGLVPYLLPVSYAPLVLGAVAATWGSRSLLHVLKATLLLVWMDAVLDPGAASLGFWIW
                     PDGGAYYGVPLSNYAGWLLSGAVATSLLLITGRWTTTPRPGLLDSATLATAFWTGVVV
                     FSGMTVPAVLGVVLFAFLLARRSHLRAAK"
     gene            1695..2561
                     /locus_tag="VHM69_07915"
     CDS             1695..2561
                     /locus_tag="VHM69_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2740356.1"
                     /translation="MTLVYIRSVILRLLHISRPVLWINTLGPATVGMWLGGDLWRWEA
                     LPVLLWLTLPFNLLIYGVNDVFDQETDAKNPRKGTFEGARIDRSEVRTIALGVVFTNA
                     PFLVYFVFFLPPSAVAWMLLYALLFVGYSVPPARFKARPYLDSLSNAAYAFPLVFMPL
                     ALGESPVWFAAVGLMAWSAAKHTFDAVQDVDEDRKVGITTTAVRLGPRGVVLWSGSLW
                     ILATACFATINVPVALLNAAIAGTLLYLLFESPAPQTGHRLYKYSIAFPYVAGTFAGV
                     QLVAALSLGTYP"
     gene            2558..3964
                     /locus_tag="VHM69_07920"
     CDS             2558..3964
                     /locus_tag="VHM69_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2740357.1"
                     /translation="MSRVVVAGGGIGGLASAALLGRAGHEVTLLEANPWLGGKSRRLE
                     LEGRRIDTGPSLLTFPGIWEEFLRRWDASEPGEAAEVAGLRLGRLSEVGRYHYREETV
                     SLPVEEGHPWHAAWERFAAIHGGLGPEVMRLLTSDPADRRTLPALSRLLRIYGTKLTT
                     RSYLDGLPWLPEGLREIIAIHTLNAGVAPARTPALYASMPAIMAADGVFVPEGGVYEI
                     VLSLERLARRAGVEVKTGEPVLQIGRGRVATTKGKYMADVIVSDLDAGRLERLLAPNR
                     KPVNETLSCSGVAVYAALRQELPDGVATHSVVLPADPDALYASLEAREEPRETMAFVN
                     YYRGGEVYPNERGTLAILLTAPANGREYALDDPFVRREIDRVSVAAGLPGPATEYFGE
                     YEVLDPAYFGAWGSAGGALYGAARPFWMSGPLHRPRYNDRGWLWRVGASVHPGGGIPA
                     VLGGAMISTGRLLRSLVK"
     gene            3994..>4355
                     /locus_tag="VHM69_07925"
     CDS             3994..>4355
                     /locus_tag="VHM69_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX2740358.1"
                     /translation="MNPGSQRDLFEIPEDIAYLNCAYMSPQLRPAREIGEKAVSRKSR
                     PWEITPDKFFEDCEEVRALFARLVGGDADGVAIVPSVSYGIAVAAANIPVREGQKIII
                     LEDQFPSNVYAWRELAAH"
BASE COUNT          802 a         1301 c         1471 g          781 t
ORIGIN      
        1 ctcatagagc tgcttgtagt cgtcgtaatc atcgggatac tcacctccat tgcgatcccc
       61 acgtacgttg gccagcagga cgaggcgaag gacgctgcag ccggggcgca actgcgtacc
      121 gcggcgacgt cacagcaact ctactatgcc aggcatgatg cgtacgcctc cgacgcagcc
      181 gacctcgaag ccttcggttt caggcaggga gagcaagtag taacggtagg gacggccagc
      241 gcgagtaact actgcatgca ggcgcaggga ggcagtggaa cattcaacat caccgaagac
      301 tcgggcaggc ccgaatcggg ctcgtgctag gaatgcagca aagaactctc gccctggcca
      361 actacgacgg agagatggaa ctcgctcgcg actttcgcta atatgttgcg agggtcgacg
      421 gcgaacttgg agggggagcc gtcgacctcc atttccagta cgcggctttt ctagagtggt
      481 tgcggatact cgccagggta ggagtcgccc gtaggttcgt tcagcccgta accgcgttgg
      541 ggatcaggca gaccgtccgc tgctgctcaa catgtccctg atgcgcctgc cgtcacggtg
      601 tccgcggtag taagaaagcc tgtcagggta ctcggtccag gcagcaaggt tgttgttggc
      661 cacgaaggtc tgagtctgcc cgaatcgccc atcctcccag ccgcttctgt aggctggatc
      721 ctcccggcga acttccaccc tcccagtggc tctttctcgg atcatctcca acctcccgaa
      781 cgaatgtctc ctacgagcat tatgtaagca caatggattc acaaatggaa gacctgaatt
      841 taataaaaat gaacaaaagt tacagaacgt ctatatatgg tgtccttgag tcgcctgaat
      901 agaaaaggtt gtgcccattg atgctatttg gccgcacgta gatgcgagcg gcgcgcgaga
      961 aggaatgcga agagtactac tccgagcacg gccgggacgg tcattcccga aaagacgacc
     1021 acgccggtcc agaatgcggt ggcgagtgta gcgctatcca gcaggcctgg ccggggggtg
     1081 gtggtccagc gacctgtgat gagaagcagg cttgtggcta ccgctcctga gagcaaccag
     1141 ccggcgtagt tgctcagcgg tacgccgtag taggcaccgc catcgggcca aatccagaat
     1201 cccagcgagg cggcgcccgg gtccaggacg gcatccatcc acacgaggag cagtgtggcc
     1261 ttgaggacgt ggagaaggga ccggctcccc caggtggcgg ccacggcccc gaggacgagt
     1321 ggcgcgtagg agacgggcag gaggtaaggg acgagcccgg cgatcctggg tcccagggcg
     1381 tcgccgtagt ggaatgggcc gtatgggaag cctgtcgcga cgccgattgc ctctacggcg
     1441 caggcgaaga cggagagcac aagcagggat agtgtggccc ggcgtggacc gaggtaacgg
     1501 aagagcgcga cggtcgaggg cagggctata aggagggtgg agacgtatga ggctgtgctc
     1561 gctcctggca cccctgggaa gcgggcggcg aagtatgccg ccacgaacga gagggcgcag
     1621 gcgaggatca ggggtcgggg cgtggcgcgg cggagctggg tgagcattcg agcaattcta
     1681 taaggtatga gtggatgacg ctggtatata ttaggtctgt gatcctgcgt ctcctccaca
     1741 tctcgcggcc ggtgctatgg atcaacaccc tcggacccgc gacggtaggg atgtggctcg
     1801 gcggggatct ctggcggtgg gaggcgctgc cggttctgtt gtggctcacg ctgcctttca
     1861 acctcctcat ctacggcgtc aacgacgtct tcgaccagga gacggacgcc aaaaatccgc
     1921 gcaaaggaac ctttgaaggc gccaggatag accgctccga ggttcgcacc atcgcgcttg
     1981 gtgtggtctt tacgaacgcg ccgtttctgg tctacttcgt cttcttcctg cctccctccg
     2041 ccgtggcctg gatgctcctg tacgcgctgc tcttcgtcgg ctactcggtc ccgccagcgc
     2101 gtttcaaggc ccgaccgtac ctcgactccc tgagcaacgc ggcctacgcc ttcccgctcg
     2161 tgttcatgcc actcgccctc ggcgagtcgc ccgtctggtt cgcggcggtc gggctcatgg
     2221 cgtggagcgc ggccaagcac accttcgacg cggttcagga tgtggacgag gaccgcaagg
     2281 ttgggatcac cacgaccgcg gtcaggctcg gccccagggg cgtcgtcctc tggagcgggt
     2341 ctctgtggat cctcgcgacc gcgtgcttcg ccacgataaa cgtgccagtc gcgctactca
     2401 acgccgcgat agcgggcacg ctgttgtacc tcctcttcga gagcccggcg ccgcagacgg
     2461 gacaccggct ctacaagtac tccatcgcct tcccttacgt ggccggtact ttcgcaggcg
     2521 tgcagctcgt cgcggcgctg tctctgggga cttacccgtg agccgggtcg tcgtggccgg
     2581 cggcggtatc ggaggtcttg cctcggcggc cctgctcggg cgcgccggtc acgaggtgac
     2641 gctgctggag gcgaatccct ggctcggcgg gaagagccgg cgcctagagc tcgaaggccg
     2701 ccggatcgac acgggaccct cgctcctcac cttccccggt atctgggagg agttcttgcg
     2761 gcgctgggac gcgtcggagc ccggcgaggc cgctgaggtc gccggtctga ggctcgggcg
     2821 gctctccgag gtcggacggt atcattaccg tgaagagacc gtctcgctgc ctgtggagga
     2881 aggccatccc tggcatgcag cgtgggagcg gttcgccgcg atacacggcg gactcgggcc
     2941 ggaggtcatg cgattactta cctctgaccc tgccgaccgc aggacgcttc ccgcgctgtc
     3001 acgtcttctc aggatatacg ggacgaagct cacgacccgc tcgtatctgg acggacttcc
     3061 ctggctgcca gaaggattga gagagatcat tgcgatccac acgctcaatg ccggggtggc
     3121 gccggcgcgg acgccggcgc tctacgccag catgcctgcg atcatggcgg ctgatggtgt
     3181 gttcgtaccc gagggcggcg tctacgagat cgtgctctcc ctggagaggc tcgcccggcg
     3241 tgctggtgtc gaggtcaaga ccggcgagcc ggtgctccaa atcggtcgcg gcagggtcgc
     3301 gacgaccaag ggaaagtaca tggccgacgt gatcgtgagc gatctcgacg caggcagatt
     3361 ggaacgcctg ctcgcaccga ataggaagcc cgtcaatgag acgctctcct gttccggggt
     3421 ggccgtatac gcggcgctca ggcaagagtt gccggacggg gtcgccacgc acagcgtcgt
     3481 gcttcctgcc gaccccgacg cgttgtacgc aagcctcgag gcccgtgagg agccgaggga
     3541 gaccatggcg ttcgtcaact actatcgcgg gggcgaagta tatcccaatg aaagagggac
     3601 gctggctata ttgctcacgg ctccggcgaa cgggcgggag tatgcactgg atgacccgtt
     3661 cgtcaggcgg gagatcgacc gcgtctcagt cgccgcggga ctgcccggtc cggctacaga
     3721 gtacttcggg gagtacgagg tactcgaccc cgcgtacttc ggcgcctggg ggagcgcggg
     3781 cggggcgctc tacggggcgg cgcgtccgtt ctggatgagc gggccgttgc acaggccccg
     3841 gtacaacgat cgcgggtggc tgtggcgggt cggggcgtcg gtgcatccag ggggcggaat
     3901 accggccgtc ctcgggggag caatgatctc cacgggcagg ttgctcagat ctctggttaa
     3961 gtagagatag actactcagg gaggacgttc gggatgaatc caggaagcca gcgtgacctg
     4021 ttcgagatac cggaagacat cgcctacctg aactgcgcgt acatgtcgcc gcagctacgc
     4081 ccggcgcggg agatcggaga aaaggcggtg tcgcgcaaat cacggccgtg ggagatcaca
     4141 cccgacaagt tcttcgaaga ctgcgaggaa gtgcgcgcgc tgtttgcccg gctcgtcggc
     4201 ggggatgccg acggcgtggc gatagtcccc tccgtcagct acgggatcgc cgtcgccgcc
     4261 gcgaacattc ccgtgcgaga gggccagaag ataattatcc tggaagacca gttcccctcc
     4321 aacgtctacg cgtggcgcga gctggcggcg cacag
//
LOCUS       DATAIR010000322         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_389637, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000322 DATAIR010000000
VERSION     DATAIR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389637"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VHM69_07930"
     CDS             complement(<1..1216)
                     /locus_tag="VHM69_07930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2740359.1"
                     /translation="MLDKETLQVVPLGGLGEIGKNMTAVRQESGMILVDAGMSFPDEE
                     MPGIDLVLPDFTYLREHPGELKGIVLTHGHEDHVGALPYLLREFNVPVYATKLTLGLV
                     RSKLQEFGIKKGDLREIKAGDTQNLGGFGLEFIEVNHSIPGAVAIAIRTNAGLILFSG
                     DYKIDLTPIEGSPMDLGRLAELGKEGVLAYFGDSTNSERPGYVPSERIVGETLNEVFE
                     KAKGRIIVASFASHIHRVSQVVEAAKKHDRLVGVTGRSMIRNVQIARDLGYLDLPQEM
                     FVDQRDINKIPDSDLVVLTTGSQGEPLAALSRIANGTHRTIEVGPEDTVVIAAHPIPG
                     NERGVSRTINNLMKRGAEVLYSPLMPVHVSGHAAQEEQKIVLSLLKPRYLVPVHGEFR
                     HLRHHANTGISLG"
     gene            complement(1294..2550)
                     /locus_tag="VHM69_07935"
     CDS             complement(1294..2550)
                     /locus_tag="VHM69_07935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2740360.1"
                     /translation="MADPNIRRKDLPGGLRVFAEPLAEATSVSLGVWIRAGSRDERDE
                     VAGISHLMEHMLFKGTPDMDALGIAQAFEGIGAQENAATGEEYTVLYARFLPEHLGRA
                     LEVMSDMILRPTLVDLEREREVIVEEIKMYEDRPDQLADEYLSSLIFHEDPLGRPIIG
                     SAETVRGVDHDTLKSFHSDTYTAKNVFVVAAGKLDPEDVERKVEEKLSGLPEGEPFVR
                     EARPTQPESRFYYKFKETEQYHVSMGSLGIPAKSEDRYAMAALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDAGAIKTYVGSTTGNVEEAVRVIVEQLAKVREESVEEEE
                     LERTKQQLKSSTLLALESTSARMNRVGRSVITGAELLSPEEISARIEAVTADDILRLA
                     REHLKTDHMYLSAVGPKELDLGRYLN"
     gene            complement(2550..>3004)
                     /locus_tag="VHM69_07940"
     CDS             complement(2550..>3004)
                     /locus_tag="VHM69_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2740361.1"
                     /translation="PRAEVSDFAPRVEVIKIPKDKIGMLIGPGGKTINALQEQFGVNI
                     SVEDDGTVFVAGEGSVAKDTAGAINGMMKDVEAGEIYTGKVVKTTNFGAFVELTPGRD
                     GLVHISRLAPGKQRVERVEDVVTEGDRIKVRVLEIDKQNRISLEKLEG"
BASE COUNT          488 a          944 c          917 g          655 t
ORIGIN      
        1 tgccgaggct gatcccggtg ttcgcgtggt gcctgaggtg acggaactcg ccgtgtacgg
       61 ggaccagata gcggggtttc aggagcgaga ggactatctt ctgctcctcc tgcgcagcgt
      121 ggccagagac gtggaccggc attagaggac tgtaaaggac ctccgcccca cgcttcatca
      181 ggttgttgat ggtacgtgag actccgcgtt cgttgcctgg tattgggtgc gccgctatta
      241 cgaccgtgtc ctcgggtccg acctcgattg tgcggtgggt accgttggcg atcctgctga
      301 gggcagccag gggttcgccc tggctgcctg tcgtcaggac cacgaggtcg gagtcgggga
      361 tcttgttgat gtcgcgctgg tcgacgaaca tctcctgggg caggtcgagg tagcccaggt
      421 cgcgcgcgat ctggacgttc ctgatcatgg agcgtcctgt aaccccgacg agcctgtcgt
      481 gtttcttggc agcctccacg acctggctta cgcgatggat gtgcgaggcg aaggaggcta
      541 cgatgatcct gcccttcgcc ttctcgaaga cttcgttgag cgtctcgccc acgatcctct
      601 cggagggcac gtagcctggt cgctcggagt tggtcgaatc gccgaagtac gctagcacac
      661 cttctttgcc aagctcggcc agtcgaccga ggtccatcgg cgaaccctca atcggggtca
      721 ggtcgatctt gtagtcaccg gagaagagga tcagacccgc gttcgtgcgg atcgctatgg
      781 cgacggcgcc aggtatggag tggtttacct cgatgaattc aaggccaaag ccaccgaggt
      841 tctgggtgtc tcccgccttt atctcgcgca agtcgccctt cttgatcccg aactcctgca
      901 acttgctgcg cacgagcccg agggtgagct tggtcgcgta gacaggtacg ttgaactcgc
      961 gcagcaaata aggcagcgcg ccgacgtggt cctcgtgacc gtgcgtgagc actatcccct
     1021 tcaactcgcc tgggtgctcc cgcaggtaag tgaagtcggg aaggaccagg tcgatccccg
     1081 gcatctcctc gtcaggaaac gacatgcctg catccaccag gatcatgcca ctctcctggc
     1141 gaacggcggt catgttcttg ccgatctcac ccaacccccc aaggggtacg acctgcaagg
     1201 tctctttgtc taacaacagt tcgtagtacc tcctttgaag gcttcaggca acaggcttca
     1261 ggaaaacctg aagcctgttg cctgttgcct gtctcagttc aggtacctgc ccaggtcgag
     1321 ctcctttggt ccgacggcgg agaggtacat atggtctgtt ttcaggtgct ctctggccag
     1381 gcgcaggatg tcgtctgcgg tgaccgcctc gatgcgggcg gagatctcct ccggcgacaa
     1441 caactcagcg cccgtaatga cgctgcggcc gacgcggttc atcctcgccg aggtgctctc
     1501 gagggcaagg agcgtcgagc tcttgagctg ctgcttcgtt cgctccagct cttcttcttc
     1561 gactgactcc tccctgactt tggcgagttg ctcgacgata accctgacgg cttcttcgac
     1621 gttgcctgtg gtcgagccga cgtaggtctt gatcgcgcca gcgtccgagt atccctggtg
     1681 gtatgagtag acggcgtatg cgagcccgcg cttctcacgc acctcctgga agaggcggct
     1741 gctcatgccg ccaccgagga cgttgttcaa cgcggccatc gcgtaacggt cctcgctctt
     1801 ggcagggatg cccagagagc ccatcgagac gtggtactgc tccgtctcct tgaacttgta
     1861 gtaaaagcgg ctctccggct gcgtcggcct cgcctcccgc acgaacggct ccccctcagg
     1921 caggccggag agcttctctt cgacctttcg ctccacgtct tcggggtcga gcttgcctgc
     1981 ggcgacgacg aagacgtttt tggccgtata cgtatcgctg tgaaaggact tcaatgtatc
     2041 gtggtcaacg ccgcggaccg tctcggcaga accaatgatc gggcgcccca gagggtcttc
     2101 gtggaagatc aaagacgaca ggtactcgtc ggcgagctgg tcgggcctgt cctcgtacat
     2161 cttgatctct tcgacgatga cctcgcgctc gcgctcgagg tcgacgaggg tcggcctgag
     2221 gatcatgtcg ctcatgactt cgagggcgcg cccgagatgt tcgggcagga agcgggcgta
     2281 gagcacggtg tactcctcgc cggtcgccgc gttctcctgg gctccgatgc cctcgaaggc
     2341 ctgcgcgatg ccgagggcgt ccatatccgg ggttcccttg aagagcatgt gctccatcag
     2401 gtggcttatg ccggcgacct cgtccctctc gtcgcgcgag ccagcccgga tccagacccc
     2461 aagggagacg ctcgtcgcct ccgcgagggg ctcggcgaag acacgaagcc cgccaggcag
     2521 gtccttccgc cttatgttcg ggtcggccac tagccctcca gcttctccag gctgatgcgg
     2581 ttctgcttgt cgatctcgag tacgcgcacc ttgatgcggt cgccctcggt cacgacgtcc
     2641 tcgacccgct cgacccgctg cttgccgggg gctaggcgcg agatgtgcac gagcccgtcg
     2701 cgacctggcg tgagctcgac gaaggccccg aagttggtgg tctttacgac cttgcccgtg
     2761 tagatctcac cggcctcgac atccttcatc atcccgttta tggcgccggc tgtgtctttt
     2821 gcgacgctcc cctcgccggc gacgaagacc gtgccgtcgt cttccaccga gatgttcacg
     2881 ccgaactgct cctgaagcgc gttgatggtc tttcctccag gccctatgag catcccgatc
     2941 ttgtctttcg ggatctttat gacctccacc ctgggggcga agtcggagac ctcggccctc
     3001 ggct
//
LOCUS       DATAIR010000323         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20689, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000323 DATAIR010000000
VERSION     DATAIR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20689"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VHM69_07945"
     CDS             complement(<1..485)
                     /locus_tag="VHM69_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX2740362.1"
                     /translation="MRSGGLLYFVILIVLAVVLVNMLSRGNPDVEELNSQQWNQAVQD
                     KSFDTDLPKTSENYLTIYDEDQTVRGLIDTGKGDPQKFEYSYTQLTDVAGQLDKADIA
                     YSVDPQNTGFWLTLLGTLAPILLIVLFFILFMSSMQGGGNRVMSFGKSRARRMTKDQP
                     KV"
     gene            complement(575..1126)
                     /gene="hpt"
                     /locus_tag="VHM69_07950"
     CDS             complement(575..1126)
                     /gene="hpt"
                     /locus_tag="VHM69_07950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2740363.1"
                     /translation="MSSMMPDVKEVLITSEEIEERVREIGARITEDYSGEKLLLIGIL
                     RGAVVIMSDLMRHIDLPCELDFMDISSYGTGTSSSGVVRILKDLEEDITDRHVLIVED
                     IIDTGLTLSYLMRSLLARKPASLEICALLSKPSRRSAELDVKYLGFEVPDEFVVGYGL
                     DYAGAYRNLPDICVLKPEVFATG"
     gene            complement(1129..2409)
                     /gene="tilS"
                     /locus_tag="VHM69_07955"
     CDS             complement(1129..2409)
                     /gene="tilS"
                     /locus_tag="VHM69_07955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX2740364.1"
                     /translation="MDLRRPLALVSGGPDSVALLRVILALGGEPVVLHVDHGLRGEES
                     REDAQFVHDLCGRLGVRCEVRQLELEDGSNLQERARDERYRLAEELATRLGLRTVATG
                     HTADDVAETVLMNLARGSGMRGISGIPPVRGKIQRPLIEITRSEILVYLKELGQPYRT
                     DPTNLTGKYARNRVRLEVLPILEDLYPGAAGNLARAASLAREDLEILEELAAGIIHRR
                     GEEVVLPFDALMELRPALRRHAVRLAYSTLLPDAPPLPSALVETVVGLVEGGEGTRTL
                     DLPGGVVVAGRAREIAMYTAQEPIANEQELRAGEEVEFGGWRISARELSAYDPVEAAR
                     AEVAYLDASKGPFGVRMAREGDIIRPLGLGGTKKVLRAMMDRKVPSDLRRRTPVVVDG
                     RGGIAWILLGELDEKFKVDESTEKVLRLEVARIS"
     gene            complement(2555..3010)
                     /locus_tag="VHM69_07960"
     CDS             complement(2555..3010)
                     /locus_tag="VHM69_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2740365.1"
                     /translation="MSEGSSSSPLKSETGNTTISSAVVSQIAGIAAQEIEKVQMGGGA
                     TAAVGGFLQSVTGGSTAGGNLTKGVSVEVGEEETAIDLTMAVEYGQSIPRISEAARRN
                     VINRVESLTGLRVTEVNITVNDVQIPEERPMLEGQEQTEEMARRQEQQA"
     gene            complement(3249..3322)
                     /locus_tag="VHM69_07965"
     tRNA            complement(3249..3322)
                     /locus_tag="VHM69_07965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3286..3288),aa:Met,seq:cat)
     gene            3395..3790
                     /locus_tag="VHM69_07970"
     CDS             3395..3790
                     /locus_tag="VHM69_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740366.1"
                     /translation="MTVVLLILILIGALISLFAAVRALGAYLRFRRARAAFQNEVTDE
                     VARLAERTTELESSISALDARAAELPIQISELQQSLSTLKVLTGALSASLRQVQKILS
                     YSALKTMRAARIGRILQSASAPRNRQHSG"
BASE COUNT          670 a         1288 c         1105 g          734 t
ORIGIN      
        1 accttcggct ggtccttggt catcctacga gcacggctct tgccgaagct catcaccctg
       61 tttccgccgc cctgcatgga gctcatgaac aggatgaaga acaatacgat gagcaggatc
      121 ggcgccaggg tgccgagcag agtaagccag aagccagtgt tctgcgggtc taccgagtac
      181 gctatatcgg ccttgtcgag ctggccggcc acgtcggtca gctgcgtgta cgagtactcg
      241 aacttctggg ggtctccctt accagtatcg atgagccctc gtaccgtctg atcctcgtcg
      301 tagatcgtca ggtagttctc gctggtcttc gggagatcgg tgtcgaatga cttatcctgt
      361 accgcctgat tccactgctg ggaattcagc tcctcaacgt ccgggttgcc ccggctcagc
      421 atgtttacca ggactacggc caagactatg aggatgacga agtaaagcag cccgccgctt
      481 ctcaaaattc tgcccaaagc ttcttacctt ccataaagtg cgtctaggac atgagaatta
      541 tactacctgc gggccgaagt gatcgttcgt tctctcagcc ggtcgcgaag acctctggct
      601 tgaggacgca gatgtcgggc aggttacggt aggcccccgc gtagtcgagc ccgtagccga
      661 cgacgaactc gtcgggcacc tcgaagccga ggtacttgac gtcgagctcg gcgctccgcc
      721 gcgagggctt ggagaggaga gcgcagatct cgagcgaggc cggcttgcgg gcgagcagag
      781 aacgcatgag gtaagagagc gtaagccccg tatcgataat gtcctcgacg atcaggacgt
      841 gacggtcggt gatgtcctct tcgaggtcct tgaggatgcg gacgacgccg ctcgatgagg
      901 tgcccgtccc gtaggagctt atgtccatga agtcaagctc gcacggcagg tctatatgtc
      961 gcatgaggtc gctcataata acgacggccc cgcgcaggat accgatcagg agcagcttct
     1021 ccccgctgta atcctcggtg atgcgcgccc cgatctcgcg caccctctcc tcgatctcct
     1081 ctgaggttat gagtacctct ttcacgtcgg gcatcatact ggacataatc acgagattct
     1141 tgccacctcc agcctcagga ccttctccgt actctcatcc accttgaact tttcgtcgag
     1201 ttccccgagc aagatccagg cgatcccacc ccgcccgtca accacgaccg gcgtcctgcg
     1261 cctgaggtcg ctcgggacct tcctatccat catcgcccgc aagaccttct tggtcccgcc
     1321 gaggcccaac ggccggatga tatccccctc gcgcgccatc cgcaccccaa atggtccctt
     1381 ggaggcgtcg aggtaggcca cctcggcccg cgcagcttcc acgggatcgt aggcggagag
     1441 ttcccgggcc gagatcctcc accccccgaa ctccacctcc tccccggccc gaagctcctg
     1501 ctcgttggcg atgggctcct gtgctgtgta catggctatc tcccttgcac gcccggcgac
     1561 gaccacgcct cccggcaggt ccaacgtccg ggtaccttcg ccgccttcca ccagaccaac
     1621 caccgtctcc acgagcgcag aagggagcgg cggcgcgtca gggagcagag tcgagtacgc
     1681 aaggcgcaca gcgtgtcgac ggagcgcggg ccgcaactcc ataagcgcgt cgaacggaag
     1741 gaccacctcc tcgcccctcc tgtgaatgat tcctgccgca agctcttcga gtatttcgag
     1801 gtcctcgcgg gcgagggagg cggcgcgcgc gagattgcca gcggctccgg gatagagatc
     1861 ttccaggata ggcaggactt cgagacgcac gcggttgcgg gcatacttgc ccgtcaggtt
     1921 cgtcgggtcc gtgcggtaag gctggccgag ttccttcagg taaaccagga tctcgctgcg
     1981 cgtgatctcg atgagggggc gttgtatctt gcctctcacc ggcgggatgc cagagatccc
     2041 gcgcatcccg ctcccgcggg ccaggttcat gagcacggtc tcggccacat cgtctgccgt
     2101 gtgacccgtc gcgacggtcc gtagtccgag ccgggtcgcc agctcttcgg cgagcctgta
     2161 tctctcgtcg cgcgcgcgct cctggaggtt ggaaccatct tcgagctcga gttgccgcac
     2221 ctcgcagcgc acgcccagtc tgccgcatag gtcgtggacg aactgggcgt cctcgcggga
     2281 ctcctcgccg cgcaggccgt ggtcgacgtg cagcaccacg ggctcgccgc cgagcgccag
     2341 gatcacccgc agcagcgcca ccgaatcggg gccgcccgag accagcgcca gcgggcggcg
     2401 aaggtccata ccgtagcgct ccgccgtccg acgcaccgta tcagggaaac gcgtggatcc
     2461 catacacaca gtttagagaa cctcgggagg ctgcaaaaca aaggaccggg acatagtgcc
     2521 ccggtcccgc attcgatggt ggtgagttac cggctcaggc ctgctgctcc tggcgccttg
     2581 ccatctcctc cgtctgctcc tgcccttcca gcatcgggcg ctcttccggg atctggacgt
     2641 cgttgaccgt tatgttcacc tcggtcacgc gcaaaccagt aaggctttcg acgcggttga
     2701 tcacgttgcg ccgcgcggcc tcgctgatcc tgggtatgga ctgcccgtac tctactgcca
     2761 tcgtcaggtc tatggccgtc tcttcctcgc cgacctcgac gctaacgccc ttggtaaggt
     2821 tcccgccggc agtgctgccg ccggtcacgc tctgcaggaa accgccgacg gccgcggtag
     2881 cgccgccgcc catctgaacc ttctctatct cctgcgccgc aataccggcg atctgggaga
     2941 cgaccgcgct gcttatcgtg gtgttgcccg tctcgctctt gagagggcta ctgctgcttc
     3001 cttcgctcat gctcgtcctt tccgacccgt ttcttcgtca gatgagtggt acccgaatgc
     3061 tgcgcaggta ctgccgtacg tctcccatcg agcctggatc cattccccac ataccgcatg
     3121 cacggctaac aggctgccta ccccgccgtt ccagattcta aaccccgcga cctactgcac
     3181 agagtcaggg cacgggaagt acgtcttgac aaaaaagaga ccccgcagtt tgcggagcct
     3241 ccgagaagta gcgggggcag gatttgaacc tgcgaccttc gggttatgag cccgacgagc
     3301 taccagactg ctccaccccg cgtcgtggga gagattctag caccggtccg aggtttgtaa
     3361 aggtggggag cgccgtgtta cggtagccgt cgagatgacc gtcgtcctcc tcatcctgat
     3421 cctgatcgga gccctgatct cgctgttcgc cgccgtccgc gcgctcgggg cgtacttgag
     3481 gttccgccgc gcccgcgcgg ccttccagaa cgaggtcacg gacgaggtcg cccgcctcgc
     3541 cgaacgcacc accgagctgg agagcagcat ctccgccctc gacgcccgcg cggcggagct
     3601 tcccattcag atctccgaac tccagcagag cctgtccacc ctgaaggtcc tgaccggggc
     3661 actctccgcc tcgcttcgcc aggtgcagaa gatcctctcc tacagcgcgc tcaaaacgat
     3721 gcgcgcagcc cgtataggca ggatcctcca gtccgcctcg gccccgagga acaggcagca
     3781 ctccggctaa agccgcc
//
LOCUS       DATAIR010000324         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_389740, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000324 DATAIR010000000
VERSION     DATAIR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389740"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VHM69_07975"
     CDS             <1..1493
                     /locus_tag="VHM69_07975"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEX2740367.1"
                     /translation="DAGVRLEDEKAVLRFAEEKLQRFLQRMSNAVRARVPDATIFYNG
                     TVTPRMAETLPYQTHFEIESLPTSSGLWGYLHYPVVARQARTFGVDFLGMTGRFHKAW
                     ADFGGLKTRDQLDYECGTIVAAGGEVSVGDQLHPRGVLDPAVYRLLEHSFGRVEKLEP
                     FLRDSKPAAEVAILSAYEPSLLGSIPMNAYVADVDGAAQLFLEAAIQFDIVDPSHGSF
                     ENYQALVLPDGMAVDGELHAKLESFLTAGGKLILSGTAALKGGTGEFGLRNVPVAYEG
                     PAPTVPSYLRLDEALAGDTELATDYDYVFYDQAHRVRPLEGATAHGELRRALFDRTWE
                     HFTSHAQAPVGDTLGSPIIVRNENVLYFAAPLFGAYRDHDYWAYREIALNAIRSFLTR
                     PLLKLSGPGWVEATLHEQPESEGHPGRRILHLVAYHPRRTTQPVQHVDQSWLTSGLGV
                     EVLTDGRAVERVYLAPGGEPLDFDVVDDYVLIGLPPVGSHAVIVLE"
     gene            1498..>1857
                     /locus_tag="VHM69_07980"
     CDS             1498..>1857
                     /locus_tag="VHM69_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2740368.1"
                     /translation="MALTDDAITGIKQMILSGQVGPGDKLPRETELATKLGLSRSSLR
                     EAVRALILAGVLKTRQGDGTYVTSLEPHQLLETMSLAVDFMRNRSLVELFEVRRMIEP
                     AATALAAGRITKEDLLRL"
BASE COUNT          335 a          606 c          598 g          318 t
ORIGIN      
        1 gtgacgcagg cgtccgcctg gaagacgaga aggctgtact ccgttttgct gaagagaagc
       61 tgcaacgttt cctgcagcgg atgagcaacg ccgtccgcgc cagggtcccg gacgccacca
      121 tcttctacaa cggcacggtc accccgcgga tggccgagac actcccctac cagacccact
      181 tcgagataga gagccttcct acctcctcgg gattgtgggg atatttgcac taccccgtcg
      241 tggcgcgaca ggcccgcacc ttcggggtgg acttcctcgg gatgacgggg cgcttccaca
      301 aggcctgggc tgacttcggc ggcctgaaga cccgcgacca gctcgactac gagtgcggca
      361 ctatcgtcgc cgccggcggt gaggtctctg tcggcgacca gctccatccg agaggcgtcc
      421 tcgaccccgc agtctaccgt ctgctcgaac attccttcgg gcgcgtggag aagctggagc
      481 cgtttctaag ggattcgaaa cccgcagccg aggtggcgat cctctcggcg tacgagccgt
      541 cgttactcgg aagcatcccc atgaacgcct acgtcgccga tgtagacggc gcggcgcaac
      601 tcttcctgga ggcggcgatc cagttcgaca tcgtcgatcc ctcgcatggc tccttcgaga
      661 actaccaggc cctcgtgctc cccgatggga tggcagttga cggggagctt cacgcgaagc
      721 tggagagctt cttgactgct ggcggcaagc tcatcctgag cggcacagcg gctctcaaag
      781 gagggacggg tgagttcgga ctcaggaatg tcccggtcgc ctacgaggga cctgctccga
      841 cggtcccgag ctacctgcgc ctcgacgagg cgctggcagg ggacaccgag cttgcgacgg
      901 actacgacta cgtcttctac gatcaggcgc acagggttcg cccgctcgaa ggcgccacgg
      961 cccacggaga gttgcgccgc gcgctcttcg acaggacgtg ggaacacttc acgagccacg
     1021 cccaggcgcc ggtcggagat acgttgggat ccccgatcat tgtgcggaac gaaaacgtcc
     1081 tctactttgc cgcgccgctc ttcggggcgt accgcgacca cgactactgg gcgtaccggg
     1141 agatcgcctt gaacgcgatc cggagtttcc tgacgcggcc gctactgaag ctctcggggc
     1201 ctggatgggt ggaggccacg ctgcacgagc agccagagag tgagggccat ccagggcgga
     1261 ggatcttgca cctcgtcgcc taccacccac ggcgcacgac gcagcccgtg cagcacgtgg
     1321 accagtcgtg gttgacctcg gggctcggcg tcgaggttct gacggatggg agagcggtcg
     1381 agcgggtata tctcgcacct ggaggcgagc cgctcgactt cgacgtggta gacgactacg
     1441 tgctgatagg tctaccgccc gtcgggtcgc acgcggtgat agtactggag tagaaccttg
     1501 gccctcaccg acgacgccat aaccggtata aagcagatga tcctttcagg ccaggtcggt
     1561 cccggcgaca agctcccgcg ggagacggag ctcgcaacca agctcgggct ctcgcgcagc
     1621 tcgctgcgcg aggccgttcg cgccctcata ctggccgggg tcctcaagac ccgccagggc
     1681 gacgggacct acgttacgag cctggagcct caccagctac tcgagacgat gagcctggcg
     1741 gtggacttca tgcggaaccg ctccctcgtt gagctcttcg aggtgcggcg aatgatcgaa
     1801 cccgccgcca ccgcgctggc cgcgggccgc atcacgaagg aagatctcct gcgattg
//
LOCUS       DATAIR010000325         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_240320, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000325 DATAIR010000000
VERSION     DATAIR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240320"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VHM69_07985"
     CDS             <1..573
                     /locus_tag="VHM69_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2740369.1"
                     /translation="VEAARRRFADEPRVLVERQDLLELQVTERVDLIFSTATFHWIKD
                     HDRLFERLARALKPGGRLVAQCGGEGNISRATKATKETMEEEIFMEYFVGWDDDKNYA
                     DAETTARRLEAAGFDDVETWLHDETAAFDSVDELARFLGTVVLGGHLERLPAAERGPF
                     AAAVAEKIVAVDGSPALDYVRLNIMARKSA"
     gene            570..1286
                     /locus_tag="VHM69_07990"
     CDS             570..1286
                     /locus_tag="VHM69_07990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740370.1"
                     /translation="MTVGALFGQAAWDYDRARRQLVPGLDRFYGAVLESVPFRTEEEL
                     RVLDLGAGTGLLSAMVAERFPRSRVTLVDLSVEMLRVARHRFPGEPGRFEFRTMDYAR
                     KPLPRGAYDLIVSALSIHHLTHGDKRELFGKVHDSLAVGGHFVNADQVRGETDEEEAR
                     YADWWLRRVREAGVSEEDLTAALSRMRADRNATLGAQLGWLLEAGFVEIGCSYRDHRF
                     AVYNGREGYESSQNGKDEDG"
     gene            1279..>2653
                     /gene="leuC"
                     /locus_tag="VHM69_07995"
     CDS             1279..>2653
                     /gene="leuC"
                     /locus_tag="VHM69_07995"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX2740371.1"
                     /translation="MDKPKTLVEKVWEKHVVRSAEGEPDLLYVDLHLVHEVTSPQAFE
                     GLRLAGRNVRRPDLTLATMDHNVPTSEFGMPIKDRVSAKQMEALRKNCEEFGIKLNEW
                     GSIGQGIVHIIGPETGLTQPGLVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     PQSRPKTMAVTVEGELPEDVTAKDLMLGILNRIGTGGGVGHVIEYRGEAVRQLSMEGR
                     MTVCNMSIEGGARAGLVAPDETTFEYLEGREYVPKGEDWDLAVEEWKKLATDEGAEFD
                     KEVVIQAEDLVPYVSWGTTPAQTVPLDGEVPEPQNDGHQRALKYMALRPGTRIRDIEV
                     DTVFLGSCTNARIEDLRAAAGVLEGHKVKEGIRAMVVPGSMRVKKQAEEEGLADIFTE
                     AGFDWRNAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPAVAAATAI
                     AGRFTTPE"
BASE COUNT          510 a          770 c          951 g          422 t
ORIGIN      
        1 gtcgaggcgg cgaggaggag gttcgcggat gagccgcggg tactggtgga gcgccaggat
       61 ttactagagc ttcaagttac tgagcgtgtg gatttgatct tctctactgc cacgttccat
      121 tggataaagg atcacgaccg actcttcgag aggctggcgc gggctttgaa gccgggagga
      181 cgcctcgtcg cccagtgcgg gggagagggc aacatctcgc gcgccacgaa ggcgacgaag
      241 gagacgatgg aagaagagat cttcatggag tatttcgttg gctgggatga cgacaagaac
      301 tacgccgacg cggagacgac cgcgagacgc ctcgaggccg cgggcttcga tgacgtagag
      361 acctggttgc acgacgagac cgcggccttc gattcggtgg acgagcttgc acgcttcctc
      421 ggtacggtcg tgctcggcgg gcacctggag aggttgcctg cggcagaacg cggtcctttc
      481 gccgccgcgg tggccgagaa gatagttgca gtggatggct cgcccgcact ggattacgtc
      541 cggctgaaca tcatggcaag gaagtcggcg tgacggtcgg agcactgttc gggcaggctg
      601 cgtgggacta cgaccgggcg cgcaggcaac tcgtgcccgg cctcgaccgc ttctacgggg
      661 ccgtgctgga gagcgtgcct ttcagaaccg aagaggaact ccgggttctc gacctggggg
      721 cggggacagg gctgttgagc gcgatggtcg ccgagaggtt cccgcgttcg cgggtgacgc
      781 tcgttgacct ctcggtggag atgctcaggg tggcgcgcca tcgcttccct ggggagcccg
      841 ggcgcttcga gtttcgtacc atggactacg ctcgcaagcc tttgcccagg ggagcctatg
      901 atctcatcgt ttcggcgctc tcgatccacc atctcaccca cggagacaag agggagttgt
      961 ttggaaaagt ccatgactcg ctcgcggttg gcgggcattt cgtcaacgcg gatcaggtcc
     1021 gtggagagac ggacgaggag gaggcgcgct acgcggattg gtggctacgg cgggtgcgcg
     1081 aggccggcgt ctcggaggag gatctcaccg ctgcgctctc gcgcatgagg gcagacagga
     1141 acgcgaccct gggggcgcag ctaggctggc tccttgaggc cggtttcgtg gagatcgggt
     1201 gtagttacag agatcacagg ttcgcggtct acaatggtag agagggatac gagagctcgc
     1261 agaacgggaa ggacgaggat ggataagccg aagacactgg tagagaaggt gtgggagaag
     1321 cacgtggttc ggagcgccga gggagagccc gacctcctct acgtcgacct gcatttggtc
     1381 catgaggtga cgagcccgca ggctttcgag ggcctcaggc tcgccgggcg gaacgtacgg
     1441 cgtcctgacc ttaccctcgc gacgatggac cacaacgtcc cgacgtctga gttcgggatg
     1501 cccatcaagg acagggtttc ggcgaaacag atggaggcgt tgaggaagaa ctgcgaggag
     1561 tttgggatca agctcaacga gtggggctcc atcgggcagg gcatcgtcca cattatcggg
     1621 cccgagaccg ggctcactca gccagggctg gtgatcgtct gcggcgactc ccacacagcc
     1681 acccacggcg ctttcggcgc cctggctttc ggcatcggga cgagcgaggt cgaacacgtc
     1741 ctcgccaccc agacgctccc gcagagccgc ccgaagacga tggccgtcac cgtcgagggc
     1801 gagctgcccg aggacgtaac ggccaaggac ctcatgctag gcatcctcaa ccgcatcggt
     1861 acgggcggcg gggtcggaca cgtcatcgaa tacagaggcg aggccgtgcg gcagctctcg
     1921 atggagggcc gcatgaccgt gtgcaacatg tccatcgaag gcggggcgcg ggcgggcctc
     1981 gtcgcccccg acgagaccac cttcgagtac ctcgaaggca gggagtacgt cccgaagggc
     2041 gaggactggg atctggccgt cgaggagtgg aagaagctcg ctacggacga aggcgccgag
     2101 ttcgacaagg aggtcgtcat acaggcggag gacctcgtcc catacgtctc ctggggaacg
     2161 accccggcgc agaccgtgcc gctcgacggc gaggtccccg agccccagaa cgacgggcac
     2221 cagagggccc tcaagtacat ggcgctccgg cctgggacca ggatcaggga catagaagtg
     2281 gacaccgtct tcctcgggtc ctgcaccaac gcgcgcatcg aggacctgcg cgcagcggcc
     2341 ggcgtcctcg aaggccacaa ggtcaaggag ggcataaggg cgatggtcgt cccaggctcg
     2401 atgcgcgtga agaaacaggc tgaggaagag ggactggccg acatcttcac agaggcgggc
     2461 ttcgactggc gtaacgcagg ctgctcgatg tgcctcggca tgaaccccga catcctgcag
     2521 cccggcgagc gctgcgcctc gaccagcaat cgcaacttcg agggacgcca gggcaagggc
     2581 gggcgcacgc acctcgtctc gccggcggtg gccgcggcga ccgcgatcgc gggccggttc
     2641 acgacgccgg agg
//
LOCUS       DATAIR010000326         9928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_20817, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000326 DATAIR010000000
VERSION     DATAIR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20817"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VHM69_08000"
     CDS             complement(<1..563)
                     /locus_tag="VHM69_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2740372.1"
                     /translation="MDTERQRSTESGIEVKPVYRPDDLEGFDYSEKLGDPGKFPYTRG
                     PYPSMYRGRPWTMRQYAGFGTARETNARFKYLTENGQTGLSVAFDLPTQMGRDSDHGL
                     ALGEVGKVGVAIDSLLDMRDLFEGIPLKEVSTSMTINATASILLLLYSLVAEEQGVEA
                     HNVTGTTQNDILKEYLARGTYIYPPGPS"
     gene            complement(575..982)
                     /gene="mce"
                     /locus_tag="VHM69_08005"
     CDS             complement(575..982)
                     /gene="mce"
                     /locus_tag="VHM69_08005"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2740373.1"
                     /translation="MLNRIYHLGYAVEDIEAASRFYAETFGAEPGVPEVVEEQGIIAT
                     MFRVGESKIELVQPTRADSPVGKFLAKRGEGFHHVAFEVDDLGKALAELKRNGVELID
                     EEPRRGAGGTRMAFVHPKGAFGVLTELVELPDG"
     gene            1068..1304
                     /locus_tag="VHM69_08010"
     CDS             1068..1304
                     /locus_tag="VHM69_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740374.1"
                     /translation="MAQSVMRKIEELSEERERLLAREGTHHTDDRGRLEQIDHDLKVL
                     WDLRRRELAGDRISLDEDFYDRYTIDPGSDAPGR"
     gene            complement(1323..1547)
                     /locus_tag="VHM69_08015"
     CDS             complement(1323..1547)
                     /locus_tag="VHM69_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740375.1"
                     /translation="MHGIQRTRKAHGPAEYGIGVTRVVVRYSDGRVMNFVADAGRDAF
                     SEDDILELKAVLDKASSTSEWADINTRLGF"
     gene            complement(1566..1787)
                     /locus_tag="VHM69_08020"
     CDS             complement(1566..1787)
                     /locus_tag="VHM69_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740376.1"
                     /translation="MEGRDRHWRGSPLDVSESTMDPYGIKEVVIEFDNGDEDILRPKR
                     RKEFESYELHQLAAYVDSLAHSIRKGKKN"
     gene            complement(1861..2211)
                     /locus_tag="VHM69_08025"
     CDS             complement(1861..2211)
                     /locus_tag="VHM69_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HEX2740377.1"
                     /translation="MAYTSPLARSRDSDQCLLALKSALDFIAALTFASVLGWGMLLSA
                     GTVLVVQGSLTLGAGFLDAVVTGTMISATTATGGVLIFGLGLLDVREVRVAKMLPALV
                     FAPLLVAATPMWPF"
     gene            2708..3460
                     /locus_tag="VHM69_08030"
     CDS             2708..3460
                     /locus_tag="VHM69_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740378.1"
                     /translation="MATIDGWLEGFLDGPAPLGLILIVSLILGLRHASDPDHLAAVTT
                     LIASEKHSKPARKAGFMGLLWGLGHGTTLVALGLPLMLAGRHLPGAVQETAETLIGVL
                     IVLLAARLLLRWRRGLFHTHPHTHEIIHAHVHSHRENLSHSHAHSVRPRTPLAAYGVG
                     LVHGIGGSGGLTLLLLATIPDPVQASTALVIFAAGTTISMALLSTAFGLAMTGGLIGR
                     NFDRAAPVLGVLGVTFGTWYALGALGAVTYPL"
     gene            3665..5080
                     /locus_tag="VHM69_08035"
     CDS             3665..5080
                     /locus_tag="VHM69_08035"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX2740379.1"
                     /translation="MQQLTLRADKRQAAFIVIAVVVAAVALGVSSLLSPADASSHREA
                     PFISGDPQADATDLYAFRSPDDPDTVTLIANYIPLEVAPAGPNFYRFGDKVLYELNID
                     NDGDARDDLSFQFRFDSRINNRDTFLYNTGPISSLDDPDLNQEQFYSVSVLNERRAND
                     PSREGKVIGNNLQVAPANVGPVSYPDGYATVADQAIYDIGNGIKVFAGPRDDPFFADL
                     GGIFDLLGGITGEDYLAGLNTHSIAIQVPIEKLEGPDDSIIGVRTTSYRQSMSVLRPI
                     GGPDSQRNGPSSSRGPWVQLSRLDMPLVNELVIPLGDKNRWNGSKPRFDSQFLSFVQN
                     PEPAALIDSILLPNDDVPPNPRDDIVAIFLTGIPGLNQPEDVKPSSQLRLNMAIEPSN
                     APDRLGVLRGDNAGFPNGRRLADDVVDIEVQALAGATPFTPAFNQEPNRSLGDGVNAN
                     DVPFLDSFPYVAEPHDYEDLD"
     gene            5167..6525
                     /locus_tag="VHM69_08040"
     CDS             5167..6525
                     /locus_tag="VHM69_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2740380.1"
                     /translation="MSAVERFRYRWGLVAALSAFVAVMVVALMTGGDERPREEPARLP
                     VDFRPAAGTATGELIGTLQRKVRENPEDVEAHVNLANAYLQMVRETGDPSLYTKADDL
                     LDRAGKIEAQNSELLATRATLDLARHDFAAALERGMQALALDPQNARYYGIVGDAQIE
                     LGMYDDAIDSYQEMVNHRPDFASFSRVSHARELYGDPEGAIEAMEFALHAGSGAAENT
                     AWAQAQLGNLWFNSGNFEEAARHYELSAETLDGYAPALAGQAKVAAANGELDQAARLY
                     EQAFNRMPLPESAIALGDVYAEMGNRRKAEQQYRLIQGIDKLYRANGVNTDLEIALFY
                     ADHDLDLQTSLEKARSAYDARPGIHAADAMAWTLYKTGDFQEAQRYASEALELGTRDP
                     LKLFHAGMIARALGQDERAKEYLQQATDLNPHFSLLYEETASSALKDLEKEQSATPKG
                     GE"
     gene            6525..8120
                     /locus_tag="VHM69_08045"
     CDS             6525..8120
                     /locus_tag="VHM69_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2740381.1"
                     /translation="MSRRASSGPLLTCFMLAFAMLTLLPSRTAEAHPLGNFTINHYSR
                     IQFSDEAAHIKYVLDFAEIPTLQQRRDLDPDGDGKLSGAEAAAYLDAELPNLLNGLQL
                     RVAGEALPLQVLDRSASYRPGQGGLPTLRVEANLLADLPTGWEEHGASQFSDQNYEGR
                     VGWQEIVVKGGPGVVVKDSTAPTTDVSNELRSYPRDSLSSPSNHRAATFTLTTGEGTA
                     MGASKAQLTEGMGMNNSVTDRAASLISVDRLSPTVVAILILTVLLWGAGHALTPGHGK
                     TIVAAYLVGTRGTVRHAAILGLTVTLTHTAGVFALGMVTLFLSRFILPEQLYPWLGVA
                     SGILVLAVGSSLLYRRLRRVLWIGSEKRGLHTRPNGAGKDTPLHNITLVAARHHHQHA
                     HAHHSIEHHHEHANPGPHHHHVHSHLPSGADGSKMTMRGLLALGVSGGLLPCPAALVL
                     LLSAISLDRLGFGMILVIAFSTGLAIVLTAIGVLMICARKVFERFSFEARMPQLLPVA
                     SALIISIFGVLIVFGALRDVGIV"
     gene            8361..8828
                     /locus_tag="VHM69_08050"
     CDS             8361..8828
                     /locus_tag="VHM69_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740382.1"
                     /translation="MAAEENKAIQDMDHEELEAALPRLREEAAKADERVHAAMERLAE
                     TAEVPTFVEIHGDQLVVDVRGVDKAMRMLSRLRIPLEHVQGAESDPEIEHTLWRGWRI
                     PGVHLPGVRFYDVHGHRDKTLVIHLKDETYSRLIVEVEDPAEVVAKINDAVGA"
     gene            9123..>9928
                     /locus_tag="VHM69_08055"
     CDS             9123..>9928
                     /locus_tag="VHM69_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2740383.1"
                     /translation="MRSYGLETLPGIVAGTPEEARRAAETLGGTVAIKAQVLTGGRGK
                     AGGIKVVNTPEEAEEAAEQILGMDISGHTVRRVYVEGGAKIDREFYLSITVDRTAKRP
                     LILFSTEGGVEIEEVAESNPEAIVKLHVDPLLGLLPYQVREVIFAAGLEGDAAKGVGR
                     TLTNLYKAFQSLDASLVEINPLVVTEEGRVLTLDAKVTVDDSSLYRHPDIAELHDVEA
                     ADPQEQRAQEGGLQYVKLDGDVGILGNGAGLVMSTLDVVAYAGEKHGGVKP"
BASE COUNT         1963 a         3062 c         3043 g         1860 t
ORIGIN      
        1 gaaggtcctg gcgggtagat gtaggtgccg cgcgccaggt actccttgag tatgtcgttc
       61 tgggtggtac cggtgacgtt gtgcgcctcc acgccctgct cctcggcgac gagcgagtag
      121 aggagaagga ggatcgaagc cgtcgcgttg atggtcatgg aggtggagac ctcttttagc
      181 gggatgcctt cgaagaggtc tcgcatgtcc agcagcgagt cgatggcgac gcctaccttg
      241 ccgacctctc caagggcgag tccatggtcc gagtcgcgcc ccatctgggt cgggaggtcg
      301 aacgcgacgg agagtcccgt ctggccgttc tccgtgaggt acttgaagcg ggcgttggtc
      361 tccctggccg tgccgaaacc ggcgtactgg cgcatggtcc acggcctgcc acggtacatg
      421 ctcgggtagg gtccgcgggt gtacgggaac tttccagggt ctccgagttt ttcagagtaa
      481 tcgaagcctt cgaggtcgtc cggcctgtag acgggcttga cctcgatccc ggactccgtg
      541 ctgcgctgtc tctcggtgtc catcgcgtgt cctttcagcc gtctggcaat tctactagct
      601 cggtgagcac cccgaaggcc cccttggggt ggacgaaggc catcctcgtc cccccagccc
      661 cccgcctggg ttcctcgtct atcagctcga cgccgttccg cttgagctca gctagggcct
      721 ttccaaggtc gtccacttcg aaggcaacgt ggtggaaacc ctcgccgcgt ttcgccagga
      781 acttcccgac cggggagtcg gccctggtcg gctggacgag ctcgatcttc gactcaccca
      841 ccctgaacat cgtcgcgatt attccctgct cctccaccac ctcgggcact cccggctcgg
      901 ccccgaaagt ctccgcgtag aacctggagg ccgcctcgat atcctcaaca gcgtaaccaa
      961 gatggtagat cctgttgagc atacttctcc ctctactcgc ggatgggaac ttagattgta
     1021 gagcatggag atccgctaga atcaaacagg acaggaagga gacagggatg gcacagagcg
     1081 tgatgcgcaa gatagaggag ctatctgagg agcgcgagcg gctcctggcg agggagggta
     1141 cgcaccacac cgatgaccgc gggcgcctcg aacagataga ccacgacctc aaggtgctct
     1201 gggacctcag gcgcagagag cttgcgggag acaggatcag cctcgacgag gatttctacg
     1261 accgctatac gatcgacccg ggcagcgacg cgccgggccg ctaggtaatg gcggttccgc
     1321 ccttagaatc ccagccgcgt gtttatgtca gcccactctg aagtcgacga ggccttgtcg
     1381 agcaccgcct tgagctcgag tatgtcgtct tctgagaacg catcccggcc tgcgtcggcc
     1441 acgaagttca tcacgcggcc gtccgagtag cgtacgacga cccgtgtcac cccgatgcca
     1501 tattccgcgg gcccgtgcgc cttccgggtt cgctgtatgc cgtgcatact atcctccgtt
     1561 tctgctcagt tcttcttgcc tttgcgaatg ctgtgggcga gggagtccac gtaagccgcc
     1621 aactggtgca gctcgtagga ctcgaactct ttgcgtcgct tgggacgcaa gatgtcctcg
     1681 tcgccgttgt cgaactcgat gacgacttcc tttattccgt aggggtccat ggtgctctcc
     1741 gacacgtcga gcgggctgcc ccgccaatgc ctgtctctac cttccatcaa cctctccttc
     1801 aagtggcgtg cttgcctggc tctccggcca atattagagt atcggcggcg cgcacggtag
     1861 tcagaacggc cacatagggg ttgccgcaac gagcagggga gcgaacacca gcgccgggag
     1921 catcttcgcc accctgacct ctctaacgtc gagcagtccc aggccgaaga tcaaaacacc
     1981 tccagtggcg gtagtcgctg agatcatggt ccctgtcact acggcgtcca gaaaccccgc
     2041 cccgagcgtc agggagccct gcacgacgag gaccgtcccc gctgatagca gcatgcccca
     2101 cccgagcacg gaggcgaagg tgagcgcagc gatgaaatcg agtgcgctct tgagggcgag
     2161 gaggcattga tcgctgtccc gactcctagc aagaggggaa gtgtaggcca cgatggtcga
     2221 gcacgctccg gctccactct atcagtgtga ggcagtttcc ttagctaaga ccctccagag
     2281 ccgatgggtc tcgcgctgaa gaagcgcctc cgagcaggca ctcgcgagga gtgcctgctc
     2341 cacctggttc tggcttactt acgcgtttgg caagggggaa agtggcgctg cctggaatgg
     2401 gtcatggcct tttcttacgc tgagtgatcc cggcatcacc cggtgccgta gaccacttca
     2461 cagggcaggg aatcccagcc ctgaacgaga tgggtgccca agggaaaggg gcgtttagtt
     2521 gaggatcttt agccgccaaa ggccgagctt gggaacgcgc cggacaggat cccccgaccg
     2581 gcggtcacca gaaatacgtc tcctgtcact cgtagagcac tccccgacgt tgaaggagga
     2641 gattcggcac cggcgagaat tctgagtcca caaccagtcg gatcaagaac tgtagaggag
     2701 caagcgcatg gcgacgatag atggttggct cgaaggtttc cttgacggac ctgcgccact
     2761 tggtctcata cttattgtga gcctgattct gggtctccgt cacgcctcgg accctgacca
     2821 ccttgcggcg gtaacgaccc tcatcgcgtc agagaagcac agcaaaccgg cccgcaaagc
     2881 cgggttcatg ggcctacttt gggggcttgg acacggcacg acgctcgtgg ccctgggtct
     2941 gccactgatg ctcgccggcc gccatttgcc cggggccgta caggagaccg cggagaccct
     3001 gatcggcgtg ctgatcgtac tcctcgccgc gcggctgctg ctgcggtggc ggcgcggtct
     3061 cttccataca cacccccaca cgcacgagat catccacgcc cacgtccact cgcacagaga
     3121 aaatctctca cacagtcatg cccactccgt tcgcccgcgc acgccactcg ctgcgtacgg
     3181 cgttggcctg gtgcatggca ttggtggctc gggcggtctc acgttgctgc tgctcgcaac
     3241 catacccgac cccgtgcagg cgagcacggc gctcgtgatc ttcgccgcgg gaacgacgat
     3301 ctccatggca ctccttagca cagcgttcgg gcttgcaatg actggaggcc tgatcggaag
     3361 gaactttgac cgcgcggccc cggtgcttgg tgttttgggg gtgaccttcg ggacatggta
     3421 cgcgcttggc gccctgggag ccgtgacgta cccgttgtag gagaccgcgt ttccgagctg
     3481 tagacgagag acatcgcaat gggcagacat cccaccgaga tagagactag cgcttttcgc
     3541 gtgcgacggc gcgcgcttgg accccgagca cccccgagtg cctgcagacc gccgaaaggc
     3601 ggatctccag gaatgcatag accgtactcg caccaatcgt gcgggattct agaaaggaga
     3661 tagcgtgcag caactgacgc tacgagcaga caagcggcaa gcggctttca tcgtgatcgc
     3721 tgtcgtggtc gcggctgtgg cgctcggtgt ttcgagcttg ttgagcccgg ctgatgcttc
     3781 atcccacagg gaggcaccct tcatcagcgg cgatcctcag gctgacgcca ccgacctcta
     3841 cgcttttcgc agccccgacg atccggatac cgtaacgctc atcgcgaact acataccgct
     3901 tgaagtggca ccggcgggcc cgaacttcta caggtttggt gacaaggttc tctatgagct
     3961 caacatcgac aatgatgggg acgcgaggga cgatctttcc ttccagttcc gcttcgacag
     4021 ccgcataaac aaccgcgaca ccttcctgta caacacgggc ccgatctcct cgcttgacga
     4081 ccccgacttg aaccaggagc aattctactc cgtgtcggtg ctcaacgaga gaagggcgaa
     4141 cgacccctcg cgcgaaggca aggtcatcgg caacaacctc caggtcgcgc cggcgaacgt
     4201 ggggcctgtg tcctaccccg acggctatgc cacggtagcc gatcaagcca tctacgacat
     4261 cgggaatggc atcaaagtct ttgccggacc acgcgacgat cccttcttcg ccgacctcgg
     4321 cgggatcttc gacttgcttg gggggatcac gggcgaggat tatctggccg gcctgaacac
     4381 ccactccatc gccatccagg tgccgattga aaagctcgaa ggccctgacg actcgatcat
     4441 cggggtcagg acgacatctt atcgccagag catgagcgtt ctgcggccta taggcggccc
     4501 cgactcgcaa aggaacggac cgagctcctc caggggtcca tgggtacagt tgtcgcgcct
     4561 ggatatgcca ctggtgaacg agctggtcat ccccctgggc gacaagaatc gctggaacgg
     4621 ctcaaagcct cggttcgaca gtcagttcct gagcttcgtg cagaaccccg agcccgctgc
     4681 cctgatcgac agcatcctgc tccccaacga cgatgtgccc ccgaacccgc gcgacgacat
     4741 cgtggcgatc ttccttaccg ggatacctgg cctgaaccag ccggaagacg ttaagccttc
     4801 gtcccagttg cggctgaata tggccatcga gccgtcgaac gccccggacc gccttggtgt
     4861 cctgcgcggg gacaatgcgg gcttcccgaa cggacggcga ctggccgacg atgtcgtaga
     4921 catcgaggtt caggcccttg caggagcaac gccgttcacg cccgccttca accaggagcc
     4981 gaacaggagt ctgggcgacg gagtcaacgc gaacgacgtg ccgttccttg acagcttccc
     5041 gtacgttgca gagccgcacg actacgagga cctcgactag gaggaatcac atttcggtag
     5101 ggaggcagac ggtgcctccc tacccacagc agttgtaaat ttattcattc ctaaagggga
     5161 ttccagatgt cggcagtaga gagattcaga tatcgctggg gtttggtagc cgcgctatcg
     5221 gcgtttgtag ccgtaatggt cgtggccctg atgacgggtg gtgatgaaag gccccgggag
     5281 gagccggcca ggctgcctgt cgacttccgt ccggctgcgg gcaccgcgac cggcgagctc
     5341 atcggcacct tgcagcggaa ggtacgcgag aatccggagg acgtcgaggc gcacgtgaac
     5401 ctggcgaacg cctacctcca gatggtgagg gagaccggcg acccttccct gtacacgaag
     5461 gcggatgatc ttctggaccg tgccgggaag atagaggctc agaactcgga actccttgcc
     5521 acacgagcca cgctcgatct cgcgcgacat gacttcgcag cggcgttgga gcgcggcatg
     5581 caagctctgg cactggaccc ccagaacgcc cgctactacg ggatcgtcgg cgacgcgcag
     5641 atcgagctcg gtatgtacga tgacgccatc gactcttacc aggagatggt caaccaccgg
     5701 cctgactttg cttccttctc ccgtgtctcc cacgcccgcg aactctacgg cgacccggaa
     5761 ggcgcgatag aggcgatgga gttcgcgtta catgccggga gcggggcggc tgaaaacacc
     5821 gcctgggcgc aggctcagct cggcaacctc tggttcaact ccggcaactt cgaggaggca
     5881 gccaggcact acgaactctc cgctgagacc ctggacggct acgcgcctgc gctcgccggc
     5941 caggcaaagg tcgccgcggc taatggtgag ctggatcagg cggccaggct ctacgagcaa
     6001 gccttcaacc gaatgccgct gcctgaatcc gccatcgctc tcggtgacgt ttacgccgag
     6061 atgggcaacc gccggaaagc tgagcaacaa taccgcctaa tacagggaat tgacaaactc
     6121 tacagggcca acggggtcaa caccgacctc gagatcgccc tcttctacgc cgaccacgat
     6181 ctggacctcc agacgtcttt ggagaaggcg cgctctgcgt acgatgcgcg tcctggtatt
     6241 cacgcggcgg acgccatggc ctggacgctg tacaagaccg gggactttca ggaagcgcag
     6301 cggtacgcct ctgaagccct cgagttgggc acccgtgatc ccttgaagct ctttcacgcc
     6361 ggaatgatag ccagggcgtt gggccaggat gagcgggcga aggaatattt acagcaggca
     6421 accgatctaa acccgcactt ctcactattg tacgaggaaa cagcctcctc cgcgttgaaa
     6481 gacctggaga aggaacagtc ggccacgccc aaaggaggcg agtgatgtct cgtcgtgcgt
     6541 cgtccggccc gctgctgacc tgtttcatgc ttgcgtttgc catgctgaca ctgctgccgt
     6601 cgcggacggc ggaggctcac ccgctgggca acttcacgat aaatcactac agtcgaatcc
     6661 agttttcgga tgaggccgcg catatcaaat atgtcctcga tttcgccgag atccctaccc
     6721 ttcaacaaag gagagatctg gaccccgatg gcgacgggaa actctccggg gcggaggcag
     6781 ccgcctacct cgacgccgaa ctgccgaacc tgttgaacgg cctccagctt agggtagctg
     6841 gcgaagccct gcctctacag gtactggacc gttcagcctc atacaggcct ggacagggag
     6901 gattgccgac cctacgagtc gaggccaacc tcttggccga cctgccaacc ggctgggaag
     6961 agcacggcgc gagccaattc tccgaccaga actacgaggg ccgggttggc tggcaggaga
     7021 tcgtcgtcaa gggagggcca ggagtcgtcg tcaaggactc cacggcaccc acaactgacg
     7081 tatccaacga gctccggtct tacccgcgtg attctctatc gagtccatcg aaccatcgtg
     7141 cggcgacgtt cacgctcacc acaggtgaag gtaccgctat gggtgcgtct aaagctcagt
     7201 tgactgaagg catggggatg aacaacagcg tgaccgaccg ggcagcctcg ctgatctccg
     7261 tcgatcgtct gtcgccgacc gtcgtagcca tactgatcct gaccgtgctc ttatggggtg
     7321 ctggccatgc cctgacccct gggcatggca agaccatagt agcggcgtac ctggtcggaa
     7381 cccgaggtac ggtccgacac gcggccatcc tcgggctgac ggtgacgctc acccacaccg
     7441 caggggtctt cgcccttggg atggtgacgc tcttcctctc gcgctttatc ttgccggaac
     7501 agttgtatcc ctggctgggt gtcgcttccg ggatcctcgt cctcgcagta ggctcctctc
     7561 tcctctatcg tcggctacgt cgtgtgctct ggatcggcag cgaaaaacga gggttacaca
     7621 cgcgtccaaa cggggctggg aaagacacgc cgctacacaa cattacgctt gtagccgcca
     7681 ggcaccatca tcagcacgcg catgcgcacc acagcatcga acatcaccat gagcatgcga
     7741 acccaggtcc gcaccatcac cacgtgcaca gccacctgcc ctctggagct gacggttcaa
     7801 agatgaccat gcgcggcctg ctggcgttgg gcgttagtgg aggtctgtta ccgtgtccgg
     7861 cggcactcgt cctcttgctg agtgcaatat cgctcgatcg gctgggcttc ggcatgattc
     7921 tggtcatcgc gttcagcacg gggctggcta tcgtcctgac agccattggc gttctcatga
     7981 tctgcgccag gaaagtattc gagcgcttct cgttcgaagc gcgaatgccg caactactgc
     8041 cggtcgcgag tgctcttatc atctcgatct ttggagtgct gattgtgttc ggggcgcttc
     8101 gagacgtcgg aatcgtgtaa ccctgacaat aaatgccgtt tgaaaacagg tccccgcgtt
     8161 cttgggctta gagtccgcag tacagaggga cctgttctcg aaagtacacg ccacaaccct
     8221 tcggccgagc cggttggtac cgtccacagg atgcatagtt ctcagacgga tccttctcgg
     8281 gggtgcatcg ggcctctcca ggtggcatca ttcctggtgg aaaggatcga cccgcattta
     8341 aggcgggaag gaggagaaga gtggctgcgg aggaaaacaa ggccatacaa gacatggacc
     8401 atgaggagtt ggaggccgcg ctgcccaggc tgcgcgagga ggccgcgaag gccgatgaga
     8461 gggttcacgc cgccatggag cggctggccg agaccgctga agtcccgacc ttcgtcgaga
     8521 tccacgggga tcaactggtg gtcgatgtca ggggtgtgga taaggcgatg aggatgctga
     8581 gtcgcttacg catccccctg gagcacgtgc agggcgcgga atcagaccca gagatcgagc
     8641 acacgctgtg gagggggtgg cgaatccccg gcgtacacct gcccggggtc cggttctacg
     8701 acgtgcacgg ccaccgagac aagacacttg tgatccacct caaggacgaa acgtacagtc
     8761 gtttgatcgt ggaggttgaa gacccagccg aagtcgtcgc gaagataaac gacgcggtcg
     8821 gcgcttagtc ccacgcacga acgaccgggc cagcacctcg gccaccttcc tttcgggagt
     8881 ttagccccct gcctccggtg ctctacgtcc acgaggtacg gtgcacacgg caagatgaat
     8941 atggagtgga gtttctcctc cgggcctatc tgcaaaattc ccacttgcgc cttcaccgcc
     9001 gggcagagcc cgcggaatat ccgtaacgtg gcgggcgagt ctctgtataa tcaccggcgt
     9061 catggagggc ttcgcgggag tcttggatct tggatctcta cgagtatcaa ggtaaggagc
     9121 tgttgaggag ctacggtctg gagacgcttc ccggcatcgt ggcagggacg ccggaggaag
     9181 ccagacgggc ggccgaaacg cttggaggga ccgtggcgat aaaagcccag gtcctgacag
     9241 gcgggcgcgg caaggccggc ggcatcaagg tagtcaatac tccggaggag gccgaggagg
     9301 cggccgagca gatactcggc atggacatca gcgggcacac cgtaaggcgc gtctacgtcg
     9361 agggcggcgc gaaaatagac agggagttct atctctcgat cacggttgac aggaccgcca
     9421 aacggcccct gatcctgttc tccaccgagg gcggcgtgga gatcgaggag gtcgccgagt
     9481 cgaatcccga ggccatcgtc aagctgcacg tggatcctct acttggactc ttgccttacc
     9541 aggtgcgcga ggtcatcttc gccgcgggtt tggaaggaga cgccgcgaag ggagtcggaa
     9601 ggacgctcac gaacctctac aaagcattcc agagtctcga cgctagcctg gtcgagatca
     9661 accctctcgt cgttacggag gaaggtcgtg tactgacgct ggacgcgaag gtcactgtgg
     9721 acgactcgag cctgtaccgc caccctgaca tagccgagct tcacgacgtg gaggcggcgg
     9781 acccgcagga gcagcgggcg caggagggtg gcctgcagta cgtcaagctc gatggcgacg
     9841 tcggcatcct cggcaacggg gccgggctcg tgatgagcac cctcgacgtc gtcgcctacg
     9901 ccggcgagaa gcacggcggc gtcaagcc
//
LOCUS       DATAIR010000327         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_113335, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000327 DATAIR010000000
VERSION     DATAIR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113335"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VHM69_08060"
     CDS             <1..792
                     /locus_tag="VHM69_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740384.1"
                     /translation="WCVNRPGSMLLVPVGDIAQHLIAVLCFIVQNGYGLYDDVNGEQI
                     PGLERFSHLVDLEEPLPLTFIETYALTECTAELSTCCYAGMLMLQAMGLGGWMFDGID
                     RHTVLGASGDPEVPGLGFRYDTDERWSLPNPTGLPGVFEGYCPPHYPDMRSAVEAFAD
                     RKFGPGGPFHPDTPGPFKETSRVRSSAQAHSEEFKECVALMAQHIYERFGKFPGTVPS
                     MFVLTYLQAHHLDLEFYDHHFGAGAYLKTHTEHMTRWHPEGSEDG"
     gene            1033..1818
                     /locus_tag="VHM69_08065"
     CDS             1033..1818
                     /locus_tag="VHM69_08065"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740385.1"
                     /translation="MTQESRWKRDGWEANVRIGVLTPHADVGPESELRAMAPPGVAIH
                     AARVPFGAMAAGGVMDQTIPLAPVRAFAEPPHVDNAAELLAAAPLNAIVYGFTSSAYL
                     IGSEGEAEMIARLEERTRGIPVVATCGAVVEGLRVLGARRIALVDAPWFDTELNALGR
                     RYYESAGFEVVYSAPCGLSSSQAKITPSDLHAWVVEHVSTDAEAIVFGGNGFRAVGVI
                     AALEEDLGSAVVTPNQALIWAALRATNADPGSVTDYGRLFGHG"
     gene            complement(2092..2604)
                     /locus_tag="VHM69_08070"
     CDS             complement(2092..2604)
                     /locus_tag="VHM69_08070"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX2740386.1"
                     /translation="MEDAMLELFRGGALAAATLTTGLMAGLYYAYAISVMLGLSMTND
                     RTFVSAMQQINVAILNPWFFVSFFGALVFTALAGVLHLPGDWRAVLPWIVAALLLYLA
                     TLVITFGFNVPLNNELASAGNPDRIADLAAVRERFEASWVRWNIARAVASTAAFGCLT
                     WALVLHGRAL"
BASE COUNT          525 a          834 c          869 g          508 t
ORIGIN      
        1 tggtgcgtta accgccctgg gagcatgctg ctcgtcccgg taggcgacat agcccagcat
       61 ttgatcgccg ttctgtgctt catcgtccag aacggctacg gactctacga cgacgtcaac
      121 ggggagcaga tccctggtct cgagcggttc tctcacctgg tggacctgga agagcccctg
      181 ccgctgacgt tcattgagac ctacgcactc accgagtgca ccgccgagct ctcgacctgc
      241 tgctacgcgg gcatgttgat gctgcaggcc atgggcctgg gaggctggat gttcgacggc
      301 atcgaccgcc acaccgtgct cggggccagc ggcgacccgg aggtgccggg actgggattc
      361 cgctacgaca ccgacgagcg ctggtcgctg ccgaacccca cgggccttcc cggagtcttc
      421 gaaggctact gcccgcctca ctacccggac atgcgctcag ccgtagaggc cttcgccgac
      481 cggaagtttg gacccggtgg gccgttccac cccgataccc cgggtccctt caaggagacc
      541 tccagggtgc gatctagcgc gcaggcccac agcgaggagt tcaaggagtg cgtggcgctc
      601 atggcccagc acatctacga gcgcttcggg aagttccccg gcaccgtgcc gagcatgttt
      661 gtgctgacct acctgcaggc ccaccatctg gatctggagt tctacgatca ccacttcgga
      721 gcgggagcgt acctcaagac ccacaccgag catatgactc gctggcatcc agagggcagc
      781 gaggacgggt aaggggacca gaagttctcc gactcgcaga gaagagggct gaggctatct
      841 cccagccctc ttcttttgcc cttattcacc gaagcgccag acgatacatt ctcggatgct
      901 cgtcacagta agcaacagca aagatcaatc attcggggga tgctccgggt gtcaaggact
      961 tgatacaatg atagcaatat tgcgcggcga ggatgactgg cgccgccctt ttggaaagga
     1021 ggttgggctt ttatgactca ggaatcgcgg tggaagcgcg acggctggga ggcaaatgtg
     1081 cgcatcggtg tcctgacccc gcacgccgat gtcggcccgg agtccgaact gcgggcgatg
     1141 gcgccgccgg gcgtggcgat ccacgcggcg cgggttccgt tcggcgcgat ggcggcaggg
     1201 ggcgtgatgg accagacgat cccccttgcg ccggtgaggg cctttgccga gccgccccac
     1261 gtggacaacg ctgccgagct actcgcggcc gcgccgctca atgcgatcgt atacggcttc
     1321 acgagttcgg cttacctgat tggatcagaa ggcgaggctg agatgatcgc ccggctcgaa
     1381 gagcgcactc ggggcatccc tgtagtggca acctgcgggg ccgtggtgga ggggctgcgt
     1441 gttctcgggg cgaggcgtat cgcgctcgtc gacgccccat ggttcgacac cgagctcaac
     1501 gccttgggaa gacggtatta cgagtcggct ggctttgagg tggtctactc ggcgccgtgc
     1561 gggctgtcga gcagccaggc gaagatcaca ccctcagacc tgcacgcctg ggtcgtcgag
     1621 cacgtatcta ccgatgccga ggcgattgtg ttcggcggga atggattccg cgctgtaggt
     1681 gtgatagccg ctctcgagga ggacctggga agtgccgtgg tgacaccgaa ccaggccttg
     1741 atttgggccg cgctgcgggc aaccaacgca gatccgggct cggtgaccga ctacggccgc
     1801 ctgtttggcc acggctagcc acgtgtgctc atcatcctgc gagaacgtgg attggatgcg
     1861 cctctgaccc tattcacccg agtgcgtgaa agtcgaattc tgcgaacgtc gcctgcacgg
     1921 ggttctccga agttagcgca actccggtcc aacacccatg tgcgtctctg gctgagatcg
     1981 gcgtccttcg gttattatct gaccttccct gactgcctca taatacctgc acatccgttg
     2041 ggcatggggg ttactggcca atcgaaccgg gtctcagctc acgccccggg gtcaaagcgc
     2101 gcgcccgtgt aacacaagag cccaggtgag gcagccaaac gcagccgtgg atgccaccgc
     2161 gcgggcgatg ttccaccgga cccagctcgc ttcgaaacgc tcgcgcaccg ctgcaagatc
     2221 ggcgatgcgg tcggggtttc ctgccgaggc gagttcattg ttgagcggca cgttaaagcc
     2281 gaatgtgatg acgagcgtcg ccaggtatag gaggagcgca gccacgatcc aaggcagcac
     2341 agctcgccaa tccccgggca gatgaagcac tccggccaag gcagtgaaca ctaacgcgcc
     2401 aaagaagctg acgaagaacc aggggttgag tatcgccaca ttgatctgtt gcatcgcgct
     2461 gacgaaggtc cggtcgttgg tcatgctcag gccgagcatg accgagatgg cataggcgta
     2521 gtagaggcct gccatgagcc ccgtggttaa cgtggccgcg gctagcgcgc cgcctcgaaa
     2581 cagctccaac atcgcatcct ccaaagctag atcttcgtct gctcgctggt acctacacag
     2641 acagtatctc agggtacggc tgaagcggtt gcagaagcat tggccgaaag gtggattgaa
     2701 gtctactctc attcacccaa gtacctggaa ggcgta
//
LOCUS       DATAIR010000328         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_113426, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000328 DATAIR010000000
VERSION     DATAIR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113426"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..237)
                     /locus_tag="VHM69_08075"
     CDS             complement(61..237)
                     /locus_tag="VHM69_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740387.1"
                     /translation="MPEIIEIRLKALCAFIERSAATGDSGVERVIEEGHLALYRRDIE
                     LLRRRRDELQKSLE"
     gene            complement(767..1291)
                     /locus_tag="VHM69_08080"
     CDS             complement(767..1291)
                     /locus_tag="VHM69_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740388.1"
                     /translation="MKHLTIRTLEERDIEPIVAAFHDLGWDKPRSQYEIYFSEQRERT
                     RTVLVAFAGRDFAGYVTINWRPGYPPFRADGVPEIQDFNVLPRFRRQRIGTRLMDEAE
                     QKVSERSSVVGIGVGVSPDYGAAQRLYVLRGYVPDGKGLMRHGRTVRKGDRISVDDAL
                     VLYLTKSLPSGPGG"
     gene            complement(1328..1723)
                     /locus_tag="VHM69_08085"
     CDS             complement(1328..1723)
                     /locus_tag="VHM69_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740389.1"
                     /translation="MVATDVGIKCPDDARLPRGARAGVMKTNQVVRSILAGLGVAVAG
                     IPVAYVLFLLPFTWLISAAAGYGAGVLINRAGGRNGGPVAITISVVATAVPFLVVLAP
                     DLLAGAFDVRPLIAMAFAVVAAGVANRQN"
     gene            complement(1813..2214)
                     /locus_tag="VHM69_08090"
     CDS             complement(1813..2214)
                     /locus_tag="VHM69_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2740390.1"
                     /translation="MNLRKRPVYMIGIASELIGVHPQTLRMYEQKGLLRPRKSIKNTR
                     LYSQEDIDLGRYIQRLTQETGMNLAGVKKVLDLERHVARLETEIRVLREELQRKEREH
                     TRVVAEVHQSYKRELVLMPRGELARSGRKGR"
     gene            complement(2211..>2592)
                     /locus_tag="VHM69_08095"
     CDS             complement(2211..>2592)
                     /locus_tag="VHM69_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX2740391.1"
                     /translation="QAGDLYVVTRVEEHPVFRRRKDDFVVDVPVSFVEAALGAQIEVP
                     RPGGGTVKVRLPAGTQDGKQFKVRGAGAPKARTNGGERGDLIVRIGVVVPKKLKRRER
                     EILEALAEERDEDVREELFKKVGS"
BASE COUNT          443 a          851 c          791 g          507 t
ORIGIN      
        1 taggcgcacg cggacagcgg agttccttga ggggtgattc gttactcact ctacgagcaa
       61 ctactccagg ctcttctgga gctcgtctcg gcgacggcgc aggagctcga tgtcgcgcct
      121 gtagagggcc agatgccctt cctcgatcac cctctcgact ccggagtctc ctgttgcggc
      181 ggatctctcg atgaacgcgc ataacgcctt cagcctgatc tcgattatct cgggcaacgc
      241 cctcctctgt tttgccggag agtgggacaa agcagtaggc tgcgtaggac acgtcccata
      301 ccctcgggcc ggggccggcc atgtcgaagt cgatgatggc actgggtctc cggtccacga
      361 agacgaggtt gtagggcgcg aagtcattgt ggcagatcac ttctcggggc ccgaaagggg
      421 cctggagttg ccagaccgcg ctgtccgggg gagcgtaccc tacggtcgca tcgtggtaac
      481 ggcgcaggag gcgggcggct tgcacgagca cctcgtgaga ccacgcttct ggaggaagcg
      541 ggtagtttcc gacctcgccc gtaatgaaag taaggatctc ccgcccacgg tcgtcgaggc
      601 cgaggaacct gggcgcgccg tcgaagttct gtctctcgag gtgccggaga aggccgtgca
      661 ccgccgggct ccacggcccg gtggcgcggc gcacggtgtc ctctacccgc actaccggcg
      721 tggagtagcc gccggagagc acttcctcgc cgcggccgtt caaacgttac cctcccggcc
      781 cggacggcag cgatttagtc aagtagagaa cgagagcgtc gtcgacggag atcctgtctc
      841 cttttcttac agtacggccg tgacgcatca ggccctttcc atcaggaacg tagccgcgga
      901 ggacgtacag acgctgcgcc gctccgtagt ccggcgaaac gcccaccccg ataccaacga
      961 cgctagaacg ttccgagacc ttctgctccg cctcgtccat caggcgagtc ccgatccttt
     1021 ggcgcctgaa gcgcggaagc acgttgaagt cttgtatttc cgggactccg tcggcccgga
     1081 atggagggta gccgggacgc cagttgatcg tcacgtatcc ggcgaagtct ctcccggcga
     1141 aggcgaccaa gacggtgcga gtcctttctc gctgttccga aaagtagatc tcgtattgcg
     1201 agcggggttt gtcccacccg aggtcgtgga aagccgcgac gataggctcg atgtctcgtt
     1261 cttcgagcgt gcgtatcgtg aggtgcttca tcgttccggc tccgtacgag cggtcgcttg
     1321 tagctcgcta gttctgtcgg ttcgccactc cggctgcgac tacggcgaaa gccatcgcga
     1381 tcagaggccg cacgtcgaac gcacccgcga ggaggtctgg ggcaagcaca acgagaaacg
     1441 gtaccgccgt cgcaacgacg gagatggtga ttgctacggg accgccgtta cggccaccgg
     1501 cgcggttgat caggacgccg gccccgtagc cggccgcggc tgagatcaac caggtgaaag
     1561 ggaggaggaa gagcacgtat gcgacgggga ttccggctac ggcgaccccg agaccggcaa
     1621 ggatactcct tacgacctgg ttggtcttca taacgccggc ccgggcgccg cggggtagcc
     1681 tggcatcgtc ggggcacttt atccctacgt cggtggcgac catgcactct gtgcagatag
     1741 gccgcccgca ggtggcgcag gagacgcggg tctccctttt cggatgccga tagcagcgca
     1801 tgggctcgga catcagcggc ccttccgccc gctccgcgcc agctcgccgc gcggcatcag
     1861 gaccagctct ctcttatagc tctggtgaac ctctgcgacg acccgggtgt gttcccgctc
     1921 cttccgttgt agctcttcgc gcaaaacacg gatttcggtc tccaacctcg ccacgtgacg
     1981 ctccaggtcg aggaccttct tcacaccggc gaggttcatc cccgtctcct gggtcagccg
     2041 ctgtatgtag cgaccgaggt caatgtcctc ctgcgagtag agcctggtgt tctttatact
     2101 cttgcgcggg cggagcaggc ccttctgctc gtacatcctc agcgtctgtg ggtgcacccc
     2161 gataagttct gaggctatcc cgatcatata cacaggacgc tttctgaggt tcatgatccc
     2221 accttcttga agagctcttc tctgacgtcc tcgtcccgct cctcggcgag ggcttccagg
     2281 atctcacgct cccgccgctt gagcttcttg ggcacgacca cgcctattcg cacgatgagg
     2341 tcccccctct ccccaccgtt ggtccgcgcc tttggcgcac ccgcgcctct caccttgaac
     2401 tgcttgccgt cctgcgtacc ggcgggcaac ctgaccttca ccgtcccgcc cccaggcctg
     2461 ggaacctcga tctgggcacc gagcgccgcc tcgacgaagc tgacaggcac atcgaccacg
     2521 aagtcgtcct tgcgcctcct gaagacggga tgctcctcga cccgcgtcac gacgtaaagg
     2581 tcgccagcct gt
//
LOCUS       DATAIR010000329         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_298292, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000329 DATAIR010000000
VERSION     DATAIR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298292"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VHM69_08100"
     CDS             <1..807
                     /locus_tag="VHM69_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2740392.1"
                     /translation="LAYIPPELREDAGEIEAAEKGRLPNIVSIEDVRGDLHVHTNYSD
                     GKGTIESMAEAAIELGYEYLVFCDHSQSLRVANGLSPERLKQKLEAVRKADEKYGEIH
                     LFCGSEVDILRDGSLDYEDDVLAELDFVVASVHTSFGVGEKAMTERIVRAMNNPYVRT
                     IGHPTGRILNRREPYDVDVSRLIREAAATNTALELNAYVDRLDIAVPYVREAVGAGVS
                     ITIDTDAHDERALSYMKYGVSQARRAWVEKENVINCLPFGEFEEYLKNGK"
     gene            807..1577
                     /locus_tag="VHM69_08105"
     CDS             807..1577
                     /locus_tag="VHM69_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX2740393.1"
                     /translation="MEDPFAGLAERYNSTGNILRQVVRHELVDRGLSENLPGPPASIV
                     DVGGGAGQQAIPLSRKGHEVTILDPSPKMLAEARRRLAREDEDVRRRVRLVEGTGERA
                     HETLGGETFDAVLCHGVLMYLEDPRPMIRALSALLRPGGILSILAKNAAALAARPALE
                     GRYHDALSSMRADRDRGRLGVVTRGDTVEHLHGAFREAGIEAVQWYGVRVFTDHLGDR
                     MPGEDLSEILELEWEAGRREPYRSVARLIHLVGRKKPV"
     gene            1718..2920
                     /locus_tag="VHM69_08110"
     CDS             1718..2920
                     /locus_tag="VHM69_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2740394.1"
                     /translation="MSVSGIEHTNGEVRNHTQLLVIGAGPYGLATAAYARRAGIEPLV
                     VGQPMAFWRDSMPSGMFLRSGAGWHLDAAREYTLTAYLEERGIDPDAVDPIPVGLFIE
                     YATWFQEKSGVDVLPEMVGDLSRTDGHFEATLQGGHRITADAVVVAPGITQFTVVPDW
                     VEKFLQPGRWSHTCTTVRFEEMRGSRVLIVGGRQSAFEWAALLADEGAAGIHVVHRHD
                     PPDFAPADWSFVDELMDLTSTIPGWFRNLPAEEREAISRRFWAEGRLKLEPWLTPRLD
                     KPFVHRWPRASVRSCRELPDGGIEVELSNSTRLVVDHVILATGYKPDMRRVPYLAGIV
                     DDLELDDGFPVLDEHFQSSLPGLFVTGFPATRDFGPFFGFVRGCPVAATLTVAGLKET
                     LDRAKSPA"
     gene            complement(2953..3393)
                     /locus_tag="VHM69_08115"
     CDS             complement(2953..3393)
                     /locus_tag="VHM69_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740395.1"
                     /translation="MSERTILLVEDDQDQVVLAMRALRKHGIVAEVDEVVVAGNGEEA
                     LDYLFCEGSHNGRDSGMMPEFVLLDVRLPKMDGLKVLERLREDERTQLLPVILFSSSN
                     EHTDVVKGYELGANSYVTKPTNFDRFSEAMLYVGWYWLNFNESP"
     gene            complement(3532..4524)
                     /gene="trxB"
                     /locus_tag="VHM69_08120"
     CDS             complement(3532..4524)
                     /gene="trxB"
                     /locus_tag="VHM69_08120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2740396.1"
                     /translation="MDQRAYDVVIVGSGPAGYTAALYAARANMNVLVFQGFESGGQLM
                     LTSDVENYPGYRDGVMGPEMMDDFEAQAARFGAEMRPENIERVDFSERPFKLWAEDED
                     EPTLAKAVVIATGAKAKWLGLESEQQLMGKGVSGCATCDAFFYRGKKVAVVGGGDTAM
                     EEAGVLAKFADEVVLIHRRDEFRASKIMLERARKNPKIEFVTDTVIEEILGEDSVEGV
                     RTKNTKTGEEQTIEVSGFFAAIGHSPATDLFKGQVEMDEGGYILQKENTMTSVPGVFA
                     AGDVSDTRYRQAVTAAGDGCRAAIDSERWLEEQGEAEGAEDPAVWTAEKDVANL"
BASE COUNT          819 a         1510 c         1400 g          810 t
ORIGIN      
        1 ctcgcctaca tcccgccgga gttaagggag gacgcgggag agatcgaagc cgcggagaag
       61 ggcaggcttc ccaacatagt ctccattgaa gacgtgcgcg gagacctgca cgtccacacc
      121 aactattctg acggcaaggg caccatcgag agcatggccg aggccgccat cgagctcggc
      181 tacgagtacc tcgtcttctg cgaccactcg cagagcctca gggtcgcgaa cggcctctcg
      241 ccggaacgcc tgaaacagaa gttggaggcc gtgaggaagg ccgacgaaaa gtacggtgaa
      301 attcacctct tctgcggctc cgaggtggac atcctcaggg acggctccct cgactacgag
      361 gacgacgtgc tcgccgaact cgacttcgtc gtggcgagcg tccacacctc tttcggggtc
      421 ggcgagaagg ccatgacaga gcgcatcgtg cgtgcgatga acaaccccta cgtacggacc
      481 atcggccatc cgacgggccg catcctcaac cgcagagaac cctacgacgt tgacgtgtcg
      541 cgtctgatcc gcgaggccgc agccacgaac acggcgctgg agctcaacgc ctacgttgac
      601 cgcctcgaca ttgcggtccc atacgtccgc gaagccgtgg gggcgggcgt cagcatcacc
      661 atcgacaccg acgcccacga cgagagggca ctgagctaca tgaagtacgg cgtctcgcag
      721 gcgaggcggg catgggtcga gaaggagaac gtgatcaact gcctcccctt cggcgagttc
      781 gaggagtacc tgaagaacgg gaagtagtgg aagacccctt cgccggcctt gccgagcgct
      841 acaactccac gggcaacatc ctgcgccagg tagtccgcca cgagctggtc gaccgcgggc
      901 tctccgaaaa cctccccggg ccgcctgcca gcatcgtcga cgtcggaggt ggcgcgggcc
      961 agcaggcgat cccactctcc cgaaagggcc acgaagtcac catcctcgac ccctctccga
     1021 agatgctcgc ggaagcaagg cgcaggctcg cgcgggagga tgaggacgtt cgccgccggg
     1081 tgcggctcgt cgagggtaca ggggagcggg cgcacgagac tctcggtggg gagacgttcg
     1141 acgcggttct gtgtcacggc gtcctgatgt atctggagga cccgcggccc atgatccgcg
     1201 ccctctccgc cctgctacgt cccggcggca tcctctccat ccttgccaag aacgccgctg
     1261 ctctcgccgc gcggcccgcc ctcgaaggcc gttaccacga cgcgctctcc tcgatgcggg
     1321 ccgacaggga ccgcgggcga ctcggggtgg tgacccgcgg cgacaccgtc gagcatctgc
     1381 acggggcatt cagggaggcc gggatcgagg ccgttcagtg gtacggggtg cgggtgttca
     1441 ccgaccacct cggggacagg atgcctggtg aggacttgtc tgagatcctc gagttggaat
     1501 gggaggccgg acggagggaa ccctacagat ccgtcgcgcg tctgatccac cttgtgggac
     1561 gcaagaagcc tgtttagggg tggtcccgga gaacaccagt tcagattctc ccgaaaaact
     1621 gcggaattag cccatttggg ggatgtgtca ttcaagtgcg taccactagg atgtgcttgt
     1681 catctgtcgt gtagcaggaa acgaggagta ttacagcatg tctgtgtctg gcattgagca
     1741 tacgaacggg gaggtccgga accacacaca actgctggtg atcggggccg gcccctacgg
     1801 tctcgccacc gcagcctacg caaggcgcgc cgggatcgaa ccactcgtgg tcggccagcc
     1861 gatggcattc tggcgcgaca gcatgccatc ggggatgttc ctgcgctctg gagccggctg
     1921 gcacctcgat gcagccagag agtacacgct gacggcctac ctggaggagc gcggaatcga
     1981 ccctgacgcc gtcgacccta tccccgtcgg gctgtttatc gagtacgcaa cgtggttcca
     2041 ggagaagtcc ggcgtcgacg tgctgcccga aatggtcggg gatctttccc gcacggacgg
     2101 acatttcgag gcgacacttc agggcggcca tcgcatcacg gccgacgccg tcgtcgtcgc
     2161 gccaggcata acccaattta ccgtcgtccc tgattgggtg gagaaattcc tccagccggg
     2221 acgctggtcc cacacctgta cgacggtccg cttcgaggaa atgcgcggtt cgcgggtcct
     2281 cattgtcggg ggacgccaga gcgccttcga gtgggccgcc ctcctcgccg acgaaggcgc
     2341 cgcggggatt cacgtagtgc accgccacga tccgcctgac ttcgcgcctg cagactggag
     2401 cttcgttgac gagcttatgg atctcacgtc caccatccct ggatggttca ggaaccttcc
     2461 cgccgaagag cgcgaggcca tatcccgcag gttctgggcc gagggaaggc tgaagctgga
     2521 gccatggctc accccgcgcc tggacaaacc cttcgtacac cgctggccgc gcgcctcggt
     2581 gcgtagctgc agagagcttc cggacggcgg gatcgaggta gaactctcca acagcactcg
     2641 actcgtggtg gaccacgtga tcctcgcgac gggctacaag ccagatatga ggagggttcc
     2701 ctacctggcc gggatcgtgg acgacctcga gctcgacgac ggcttccccg tactcgacga
     2761 gcacttccag tcgagcctgc ccggcctgtt cgtcacgggc ttccccgcca ccagggactt
     2821 cggccccttc ttcggcttcg tgcgcggctg ccccgtggcg gccacactca cggtcgccgg
     2881 gttgaaggag acgctggatc gcgcaaaatc cccggcctga ggtcccgggt cttgacggcg
     2941 ctgtaggtac ggttacggag actcgttgaa gttgagccag taccagccca cgtagagcat
     3001 ggcctctgag aacctgtcga agttggtcgg cttggtgacg taggagttgg cgccgagctc
     3061 gtagcccttc accacgtcgg tatgctcgtt ggaggaagag aagaggatca caggcaggag
     3121 ctgcgtccgt tcgtcttcgc gcagccgctc aaggaccttc aggccgtcca tcttcgggag
     3181 gcgcacgtcg agcaggacga actcaggcat cattcctgaa tcccgcccgt tgtgggagcc
     3241 ctcgcagaaa agataatcaa gggcctcctc gccgttccct gcgaccacga cctcgtccac
     3301 ctcggcgacg atgccgtgct tacggagggc gcgcatagcg aggacgacct gatcctggtc
     3361 gtcctctacg aggagtatgg tcctctcact cacaccgact cccccggtcc ccccttctgg
     3421 cggcgcatta tactcgccgt ccccgccggc gtacagtgaa cgagaaacac ccccgacgtt
     3481 ctggccgggg gtgtttctaa cgttcgaccg ggagacgcct gagaccgggt tctagaggtt
     3541 cgccacgtcc ttctcagcgg tccatacggc ggggtcctcg gcgccttccg cctcgccctg
     3601 ctcctcgagc cagcgctcgc tgtcgatggc ggcccggcag ccatcgccag ccgccgttac
     3661 ggcctggcgg tagcgcgtat cggagacgtc accggccgcg aacacgcctg ggacgctcgt
     3721 catggtgttc tctttctgga ggatgtagcc accctcgtcc atctcgacct gtcccttgaa
     3781 gaggtccgtc gctggagagt ggccgatggc ggcgaagaag ccgctcacct cgatggtctg
     3841 ctcctcgccg gtcttcgtgt tcttcgtgcg cactccctcg acggagtcct cgccaaggat
     3901 ctcctcgatc accgtgtccg tgacgaactc gatcttgggg ttcttgcgag ccctctcgag
     3961 catgatcttg gacgccctga actcgtcgcg gcggtggatt aaaacgacct cgtcggcgaa
     4021 cttggcgagg acgccggcct cttccatcgc ggtgtctccc ccgccgacga ccgcgacttt
     4081 cttgcccctg tagaagaagg cgtcgcaggt tgcgcagccg ctgacaccct tccccatcaa
     4141 ctgctgctcg ctctcgagcc cgagccactt ggccttggca cccgtggcaa tgacgacggc
     4201 cttcgccagg gtcggctcgt cctcatcctc ggcccacaac ttgaacggac gctccgagaa
     4261 gtccaccctc tcgatgttct cggggcgcat ctcggccccg aaacgcgcgg cctgcgcctc
     4321 gaagtcgtcc atcatctcag ggcccatcac accgtccctg taccccggat agttctcgac
     4381 gtccgaggtc agcattagct gcccaccgct ctcgaaaccc tggaatacca gcacgttcat
     4441 gttcgccctg gccgcgtaga gggccgcagt ataccccgca ggacccgaac ctactataac
     4501 gacatcatac gctctctgat ccatcagaaa cgctcctcc
//
LOCUS       DATAIR010000330         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_113641, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000330 DATAIR010000000
VERSION     DATAIR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113641"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..2600
                     /gene="leuS"
                     /locus_tag="VHM69_08125"
     CDS             141..2600
                     /gene="leuS"
                     /locus_tag="VHM69_08125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX2740397.1"
                     /translation="MSETPFKETSSRKRTYDPTDLERRWQERWAESGLYKTDEDESKP
                     KHYALTMLPYPSGDLHVGHWYPMTPSDTRARYMRMNGYRVFFPIGFDAFGLPAENAAI
                     QRGINPREWTYSNIERMRGHLRQMGAMFDFDSEVVTCDPEYYRWNQWFFLKFLENGFA
                     YREEAAVDWCPKDNTTLAREQVIGPDRRCERCGTPVIKKYLAQWFFKITDYAEELLDY
                     SKIDWPERVQTLQRNWIGRSEGTEIRFEIEGYTAIEVFTTRPDTLFGATFFVMSPEHP
                     AVEEITTPDQAEGVRAYVERAGRMSEIDRTDVTREKTGVFTGAYATNPANGEKVPIYI
                     ADYVLMGYGTGAIMAVPAHDDRDFEFAKRHGIEIRTVIAPPDWDGEDLEEPYVGEGTM
                     VNSGQFDGTPSTEGKENVTTWLDERGVGEATVTYRLHDWLISRQRYWGTPFPIVYCDE
                     CGIVPVPEEDLPVLLPEESEFMPTGVSPLKLDPDFYNTDCPRCGGPATRETDTMDTFM
                     DSSWYWYRYLSPHYDEGPFDPVRGEKWLPVDQYTGGIEHAILHLLYARFFTKVMRDLG
                     LVDFDEPFVRLRNQGIILGPDGQKMSKSRGNVVNPQEYVDRYGSDALRCFLMFIGPWN
                     EGGPWDSKGIEGVSRWFRRALSLVTEADSSGGNADPSDLTRMTNRLVKKITGDLEDFR
                     FNTAIAALMEHTNYLLAIKGRVEDDEWQEALRTFVTVLAPFAPHHAEEMWAAMGKEYS
                     VHEQAWPGFDEALIQAEEITLIVQVNGKLRDRIEAPADVSEEAAMELALSSTRVRPHI
                     EGKELRKSVYVPGRLVNLVVG"
BASE COUNT          574 a          796 c          830 g          455 t
ORIGIN      
        1 ggcgtaagtg gggatggtac acttacgaga tgcgcgctat tgaggacaat tccgtcgtct
       61 tcgatctccg actataagct cgcgcctttc cctgttccga aatcaggcaa gacccctcga
      121 aagagcaaga ggagttactc gtgagcgaaa caccgtttaa agagacatcg tccagaaaga
      181 gaacttacga tcccacggac ctcgaacgcc gttggcagga gcggtgggcg gagagcgggc
      241 tgtacaaaac cgacgaggac gagagcaagc cgaagcacta cgcgctcacg atgctcccat
      301 atccaagtgg agacctgcac gtggggcact ggtacccgat gacgccctcg gataccaggg
      361 cgcgttatat gcggatgaac ggctacaggg ttttcttccc gataggcttc gacgccttcg
      421 ggcttcctgc cgagaacgct gcgatccaga ggggcataaa tccccgcgag tggacctact
      481 cgaacatcga gcggatgcgc ggccacctca ggcagatggg cgcgatgttc gacttcgatt
      541 ccgaggtagt gacctgcgac cctgagtact accgctggaa ccagtggttc ttcctcaagt
      601 tcttggagaa cggattcgcc taccgtgagg aggccgctgt tgactggtgt ccaaaggaca
      661 atacgacgct ggcccgcgag caggtcatag ggcccgacag gcgctgcgag cggtgcggga
      721 cgccggtcat caagaagtac ctcgcgcagt ggttcttcaa gatcacggac tacgctgaag
      781 agctcctgga ctactcgaag atagactggc ccgagcgggt ccagacattg cagagaaact
      841 ggatcgggcg ctccgagggg accgagataa ggttcgagat cgagggctac acggccatcg
      901 aggtcttcac cacccgcccg gatacgctct tcggcgcgac gttcttcgtg atgagcccgg
      961 agcatcctgc agtcgaagag ataactactc ccgaccaggc cgaaggcgtg cgtgcctacg
     1021 tcgagagggc cggcaggatg agcgagatcg accgcaccga cgtgacgcgg gagaagacag
     1081 gcgtgtttac gggtgcttac gcgaccaacc cggcgaacgg agagaaggtc cccatctaca
     1141 tcgccgacta cgtcttgatg ggctacggca caggcgcgat catggccgtt cccgcccacg
     1201 acgaccgaga cttcgagttc gcgaagaggc acggcatcga gatcaggacc gtgatagcgc
     1261 cgccggattg ggacggcgaa gacctcgaag aaccctacgt cggcgagggc acgatggtca
     1321 actcgggcca gttcgatggc accccaagca ccgagggcaa ggagaacgtg acgacctggc
     1381 tcgacgagcg cggagtcggc gaggctacgg tcacatacag gcttcatgac tggctcataa
     1441 gccgccagcg ttactggggt accccgttcc ctattgtcta ctgcgacgag tgcggcatcg
     1501 tccccgtccc ggaagaagac ctgccggtgc tgctccccga ggaatcggag ttcatgccga
     1561 cgggcgtatc acccttgaag ctggatccgg acttctacaa cacggattgc ccccgttgcg
     1621 gaggcccggc cacgcgcgag acggacacga tggacacctt catggactcc tcgtggtact
     1681 ggtaccgtta cctctcgccg cattacgacg aggggccttt cgatcccgtg cggggagaga
     1741 agtggcttcc cgtggatcaa tacaccggcg gcatcgagca cgccatcttg cacctgctct
     1801 acgcccgctt ttttaccaag gtcatgcgcg acctgggtct ggtggacttc gacgagcctt
     1861 ttgtccgcct gcgcaaccag gggatcatac tcgggcccga cgggcagaag atgagcaaga
     1921 gccgtggcaa cgtggtaaac ccgcaggagt acgtggaccg ctacggctcc gacgcgctcc
     1981 ggtgcttcct gatgtttatc ggcccgtgga acgaaggcgg accctgggat agcaagggca
     2041 tagagggtgt ctcgcgatgg ttcaggcgcg ccctctcgct cgtgacagag gctgactcct
     2101 cgggcggcaa tgcggacccg tcggatctca cccgcatgac gaaccggctt gtaaagaaga
     2161 tcacgggcga cctcgaagac ttccgcttca acaccgctat cgccgccctc atggagcaca
     2221 cgaactacct gctcgcgatc aaaggccgcg tcgaggatga cgaatggcaa gaagcgctgc
     2281 ggacctttgt caccgtcctc gccccgtttg cgccgcacca cgccgaggag atgtgggccg
     2341 cgatggggaa ggagtactcg gtccacgagc aggcgtggcc cggcttcgac gaagccctta
     2401 tacaagccga agagatcaca ctcatagtcc aggtgaacgg caagctgcgc gaccgtatcg
     2461 aagcccccgc agatgtctcg gaggaggcgg caatggaact cgcgctctcc agcaccaggg
     2521 tccgtccgca tatcgaaggc aaggaactcc gtaagagcgt ctacgtgcca ggaagactcg
     2581 tcaacctcgt cgtcggttag agcagggttc tccaacggga gtagacgggg aacggaccgt
     2641 atcgccgcag cctgc
//
LOCUS       DATAIR010000331         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_332482, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000331 DATAIR010000000
VERSION     DATAIR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332482"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..1225
                     /locus_tag="VHM69_08130"
     CDS             440..1225
                     /locus_tag="VHM69_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX2740398.1"
                     /translation="MLRTVTATRYVTPLREGGSLPAIVEADDLGTYVLKFRGAGQGRK
                     ALVAELVAGEIARHLGLLVPEVVLAYLAPELGRSEPDPEIQDLLKASPGLNLALDYLP
                     GSLGFDPLVEPPDSGLASRIVWFDALVTNVDRTPRNTNLLVWHSRLWLIDHGASLYFH
                     HNWPARDGTALETASQRPFAAARDHVLLPFAGSIPEADADLAPLLTNDVLNAIVDLIP
                     DQWLDDEPGFGSADEVRSAYVAYLSKRLREPRTWVGALEEAAR"
     gene            1222..1605
                     /locus_tag="VHM69_08135"
     CDS             1222..1605
                     /locus_tag="VHM69_08135"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX2740399.1"
                     /translation="MSLFQYALLRVVPRVERGEFINAGVVLYCQDAKFLDARIHLDPE
                     RLRALDPNLDAEAVQAHLDVARLVCEGGPGAGAVGLLPPGQRFGWLVAPRSTVVQPSP
                     VHTGFADDPEEVIEHLLRVMVRTTE"
     gene            complement(1718..2305)
                     /locus_tag="VHM69_08140"
     CDS             complement(1718..2305)
                     /locus_tag="VHM69_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740400.1"
                     /translation="MLIYPLDLSFKIVTIGTRIRVTDATGRQVAYVRKKKFKLKEEVR
                     VYEDEAQSRLLFRMKADRMIDFSARYAVSGPDGHPIGAVGRQGMKSLWNSAYVLTDGY
                     GEQTGSIREENAWVKVLDGMMESVPFADALGGLFFNPAYLAELRGQNVLRVQKQRSIL
                     ESSFHIEKLADFSEDEEDLLLAGIIMMVLLERDRG"
     gene            complement(2299..2583)
                     /locus_tag="VHM69_08145"
     CDS             complement(2299..2583)
                     /locus_tag="VHM69_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740401.1"
                     /translation="MLETRKSASPVRVKDLAAGTGSRHVTDLLLGVLGSIEPYETCIL
                     EAERDGDLEVAAFLRELRRQDLARAREATRLLRRSLEAAHLNVGEERVVC"
     gene            complement(2701..3123)
                     /locus_tag="VHM69_08150"
     CDS             complement(2701..3123)
                     /locus_tag="VHM69_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2740402.1"
                     /translation="MEQNDHNYGVAEERGPVTMTTQEERTWSVLAHLSIYLNLVTGFL
                     GPVAALFIWLVYRDRSRKVAFQALQSMWYQVAWLVILVSGWTVTTLLMLVLIGFLLVP
                     VMALITVVPFVHSAYAAYRVSKDGDYRYPLIADMIEAR"
     gene            3281..3943
                     /locus_tag="VHM69_08155"
     CDS             3281..3943
                     /locus_tag="VHM69_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740403.1"
                     /translation="MHRTHDNDEARSTNDGHRIFAALYGSLGFCIERLWMGEHRPRLL
                     KGASGAVLEIGGGTGANLPHYSDVERVTVAEPDPFMRKRLIPKLGAARVPVEVSAAGA
                     EALPFHDGSFDTVVSTLVLCTVPDQGSALEEIRRVLRPGGRLLFIEHVRATGSIARWQ
                     DRIEPFWRRLFGGCHPNRDTVAAIEEVGFEIESFESFYPPVPRSGLIPHVQGSATVRL
                     AA"
BASE COUNT          749 a         1363 c         1216 g          795 t
ORIGIN      
        1 agcgaagaga gcgcctccgc cccgatccca tcgtcggcgc gcaagatccc cagcaagaga
       61 tcctcgtctc ccacagagtc gtgcccaagc atcatcgcct cctccacagc ggccaccaca
      121 cacctgcggg cttcattggc aaaacggttg aatatcatgc gtcccttctc ctcaatggct
      181 ctccagggta cttgtccccg atcttcctgg cacagcccac ttgcttacca aaacctcggc
      241 tacgaccact ccttcgagac cctccctcgg atcatccgat ccacccgagc ttgtcctcat
      301 catctcattc atgcgtcaat catagttgac gctgtccggc ttgtcaacca tagttgacag
      361 acatgacatt ctgcgttggc aatatgtact attcgtgggt gcgcaatgac tgggatcgtc
      421 gtcttcggga gactttggct tgctccgcac cgtgacagca acgcgctacg ttacgccctt
      481 gcgcgagggg ggttcgctgc cggcgatcgt cgaggccgac gaccttggga cctacgtact
      541 caagttccgt ggggccggac agggccgaaa ggctctggtg gccgagctcg tggccggtga
      601 gatagcgcgc cacctcggcc ttctcgtacc cgaggtcgtg ctcgcctacc tcgcccccga
      661 gctgggccgc tcggagcctg acccggagat ccaggacctc ctgaaggcga gccccggact
      721 gaacctcgcg ctggactacc tgccgggctc cctgggcttc gacccgctcg tcgagccgcc
      781 cgactccggc ctcgcctcgc gcatcgtctg gttcgacgcc cttgtgacta acgttgaccg
      841 caccccgcgc aacacgaacc tcctcgtctg gcacagtcga ctctggctca tagaccacgg
      901 cgcgtcgctg tacttccatc acaactggcc cgcacgagac ggaacggccc tggaaacggc
      961 gagccagcgt cccttcgcgg cagccaggga ccacgtgctt ctcccattcg ccgggtccat
     1021 cccagaagcc gacgccgatc tcgccccgct gctcaccaac gacgtgttga acgccatcgt
     1081 agatctgatc cccgaccagt ggctggacga cgagccgggt ttcggtagcg ccgacgaagt
     1141 acgttctgcc tacgtcgcct acctgagtaa gcgacttcgg gaaccgagaa cgtgggtcgg
     1201 ggcgcttgag gaggccgcta gatgagcctg tttcagtacg ccttattgcg agtggtgcca
     1261 cgtgtggagc gcggggagtt cataaacgcg ggcgtggttc tctactgcca ggacgcgaag
     1321 ttcctcgacg cgcgcatcca cctcgacccc gaacgtctgc gggcgctcga cccgaacctc
     1381 gacgccgagg ccgtgcaggc gcacctcgat gtcgcgcgcc tggtgtgcga gggtggaccg
     1441 ggggccggcg cggtgggcct gctgccaccc ggccagcgtt tcggctggct cgtcgcgccc
     1501 cgcagcaccg tggtgcagcc ctctcctgtc cacacgggct tcgcggatga cccggaagag
     1561 gtcatagagc acctcctcag ggtcatggtc cgtacgacgg agtagtagcg aaaactccca
     1621 ccctcgctcc ccagtaaagg ctgacaagct gagaagccgc ctggggtccc caggcggctt
     1681 gctgaggagc gcgcggaagg aacttccgca aggcgcttta gccgcggtcc ctctcgagta
     1741 gcaccatcat gatgattccg gcgaggagca aatcttcctc gtcctctgag aagtccgcga
     1801 gcttctcgat gtggaagctg ctctccagga tggagcgttg tttctggacg cgaagtacat
     1861 tctggccacg aagctcggcc aggtaggcgg ggttgaagaa gagaccgcca agggcgtcag
     1921 cgaatgggac ggattccatc attccgtcga ggaccttgac ccaggcgttc tcctcccgga
     1981 tcgagccggt ttgctcgccg tacccatcgg tcaggacgta ggcgctattc cacagggact
     2041 tcattccctg ccgcccgacc gctccgatgg ggtgcccatc aggtccactt acggcgtacc
     2101 ttgcgctgaa gtcgatcatc ctgtcggcct tcatgcggaa gagcagacgg ctctgggcct
     2161 cgtcctcgta gaccctcact tcttctttca gcttgaactt cttcttgcgc acgtacgcga
     2221 cctggcgccc cgtggcatcg gtcaccctga tgcgagtccc gatcgtgacg atcttgaagc
     2281 taaggtccag cggatagatc aacatacgac tctctcctct ccgacgttta aatgagccgc
     2341 ttccagggat cttcgtagaa ggcgagtcgc ctccctggcc ctggcgaggt cctggcgacg
     2401 caactcgcgc aggaaggccg cgacctccag gtcaccgtcc cgctcggcct cgaggatgca
     2461 ggtctcgtag ggttcgatgc tcccgagcac tccgagcagc aagtccgtta cgtggcggct
     2521 ccccgtgccg gctgcgagat cctttaccct caccggcgaa gcgctcttcc tggtttccag
     2581 catcgctatc ctcctgaagc actagcattt aatacgcacg acgttggcga atagcgtcag
     2641 accacccgcc tggagcggcg cccccgacga ggtcggaggc gccggttccg tcagagccgt
     2701 ctagcgggct tcgatcatgt cggcgataag cgggtaacgg taatctccgt ccttgctcac
     2761 gcggtatgcc gcgtatgcgc tgtgcacgaa ggggaccacc gtgatcaggg ccatcacggg
     2821 gacgagcagg aacccgatca agactagcat caagagcgtc gtcacggtcc atcctgagac
     2881 caggatcacc agccaggcca cctggtacca catcgactgc aaagcctgga aggccacctt
     2941 cctcgacctg tccctgtaga cgagccagat gaacagggcc gccaccggtc cgaggaaccc
     3001 tgtaaccagg ttgaggtaga tgctcaggtg cgcaagcacc gaccaggtcc tctcctcctg
     3061 cgtcgtcata gtcaccggac cccgctcttc agctactccg taattgtggt cgttttgctc
     3121 catctcttct cctcgctgtt gctcgatacg ctatatcgag aaaatactcc cggatagagc
     3181 tttcggcatc ggtcgcggga cctaatgttc gtaggtccgc ggtcccagct ttctaagatg
     3241 gcggagaagc cccgagcatg gcagactcta tagagagccg atgcacagaa cccacgacaa
     3301 cgacgaagcc cgctccacga acgacggaca tcgcatcttc gctgcgctct acggttcgct
     3361 tggcttctgc atcgaacgat tatggatggg cgagcacagg ccgcgattgc tcaaaggggc
     3421 cagcggcgca gtgctggaga tcgggggcgg tactggagcg aacctcccac actacagcga
     3481 cgtggagcgg gtaaccgtcg ccgagcccga tcccttcatg cggaagaggc tcattcctaa
     3541 gctcggggcc gcccgcgtac ccgtggaggt ctcggctgcg ggcgctgaag cgctgccttt
     3601 ccatgacggg agctttgata cggtggtctc gacgctcgtc ctatgtacgg tcccggatca
     3661 gggatcggca ctggaggaga tccggcgcgt cctgcgtccc ggcgggcggc tgctattcat
     3721 cgagcacgtt cgggcaacag gctccatagc ccgctggcaa gaccgcatcg agcccttctg
     3781 gaggcggctc ttcggtggct gccacccaaa cagagatacc gtcgcggcta tcgaggaggt
     3841 agggttcgag atagagtcgt tcgagagctt ctatccgccg gtcccccggt ccggcctcat
     3901 accacacgtc cagggctcgg cgaccgtcag gctggccgcc tgacaaaagg ccggtatgct
     3961 aggatcaaac gccgatgagt accctgacgc attcttcatc cagatgatga gccgcgggac
     4021 gggcctggtt ttggccctcg cgctcgcgct cgtcgtcttc ttcgtggacc agtggatcaa
     4081 atccgtcgtc gaaggctcga tgcgggtagg ggagagcatc acc
//
LOCUS       DATAIR010000332         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_298560, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000332 DATAIR010000000
VERSION     DATAIR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298560"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..558)
                     /gene="rplE"
                     /locus_tag="VHM69_08160"
     CDS             complement(16..558)
                     /gene="rplE"
                     /locus_tag="VHM69_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2740404.1"
                     /translation="MARMKDRYNEEVAPSLKERFGIDNPMRIPGLEKIVVNMGVGEAV
                     VNSRALDGAMEDLARITGQKAQLRRARKSIAGFKIREGMAVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGVSPASFDGRGNYAIGLKEQVIFPEISYDSIDATRGLDVAVVTTT
                     ESDEEARELLRLLGMPFRQS"
     gene            complement(551..868)
                     /gene="rplX"
                     /locus_tag="VHM69_08165"
     CDS             complement(551..868)
                     /gene="rplX"
                     /locus_tag="VHM69_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2740405.1"
                     /translation="MGLKLKRGDTVVVISGKEKGKRGEVERVIPKENRVVVGGVNVRT
                     RHARPSQQNQQGLYHFEAPIHVSNVMLVDPNSGEPTKVGYRFTDSGEKLRVGKKSGKD
                     IDG"
     gene            complement(871..1239)
                     /gene="rplN"
                     /locus_tag="VHM69_08170"
     CDS             complement(871..1239)
                     /gene="rplN"
                     /locus_tag="VHM69_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX2740406.1"
                     /translation="MIQQESRLRVADNSGARSILTIRVLGGSKRRTAGVGDVIVATVK
                     EATPGSAVKKGEVVRAVVVRTKKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELREKGYTRIISLAPEVL"
     gene            complement(1239..1505)
                     /gene="rpsQ"
                     /locus_tag="VHM69_08175"
     CDS             complement(1239..1505)
                     /gene="rpsQ"
                     /locus_tag="VHM69_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX2740407.1"
                     /translation="MQTDTERNRRKERVGIIVSDKAEKTVTVAVEALVRHPKYKKRVR
                     RSKKFIVHDERNEASVGDTVRIIETRPLSARKRWRLANIISRAE"
     gene            complement(1658..1855)
                     /gene="rpmC"
                     /locus_tag="VHM69_08180"
     CDS             complement(1658..1855)
                     /gene="rpmC"
                     /locus_tag="VHM69_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX2740408.1"
                     /translation="MKVAEVRELDVQELERRLAETRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARILTVLNQKQQESG"
     gene            complement(1852..>2079)
                     /gene="rplP"
                     /locus_tag="VHM69_08185"
     CDS             complement(1852..>2079)
                     /gene="rplP"
                     /locus_tag="VHM69_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX2740409.1"
                     /translation="WINIFPHKPVTKKPAETRMGSGKGSPEAYVAVVKPGRVMFEMSG
                     VDETLAREAMHLAAQKLPIKTRFMVRGGGQQ"
BASE COUNT          304 a          738 c          579 g          458 t
ORIGIN      
        1 gtcataccct acctctcaac tctgcctgaa cggcatcccg aggagccgta gtagctcccg
       61 cgcctcttcg tcgctctccg tcgtcgtgac gaccgccacg tccagcccac gggtcgcgtc
      121 tatggagtcg taggagatct ccgggaagat aacctgctcc ttgagcccga tcgcgtagtt
      181 cccgcgcccg tcgaaggagg cggggctcac gcccctgaag tccctcacgc gcggcaacgc
      241 tatggagata aagcggtcta ggaactccca catgcgctcg ccgcgcagcg tgaccctcgc
      301 cccgaccgcc atcccctcgc ggatcttgaa ccctgcgata gacttgcggg cccgcctgag
      361 ctgggccttc tggccggtga tcctggcgag gtcctccatc gcgccgtcga gtgcgcggct
      421 gttcaccacg gcctccccga cacccatgtt gaccacgatc ttctcgagac caggtatgcg
      481 catggggtta tcgataccga acctctcctt gaggctcggg gcgacctcct cgttatatct
      541 gtctttcatc ctagccatcg atatccttcc cggacttctt gccgacccgg agcttctccc
      601 ccgaatccgt gaaacggtat ccgaccttcg tcggctcgcc ggagttcggg tcgaccagca
      661 tcacgttcga gacgtgtatg ggcgcctcga agtggtagag tccctgctgg ttctgctggc
      721 tcggcctagc gtggcgcgtg cggacgttga cgccgccgac gacgacccgg ttctccttcg
      781 ggatgacccg ctcgacctcg ccgcgtttgc ccttctcttt accggagatc accacgacgg
      841 tgtccccgcg ctttagtttg agacccataa ctacaacacc tccggagcga gcgagatgat
      901 cctggtatag cccttctccc tgagctcgcg cgccacgggc ccgaagatac gcgtcccgcg
      961 gggggcgcca tcagggttga taatgacgcc cgcgttctcc ccgaagcgga tgtaggaccc
     1021 atcgtcgcgg cgcgtctcct tcttggtccg caccaccacg gcccgcacta cctcaccctt
     1081 cttgacggcg ctgccaggcg tggcctcctt gacggtggcg acgatgacgt cgcccacgcc
     1141 agcggtgcgg cgcttgctcc cgccgagcac gcgtatggtc aagatgcttc tcgcacccga
     1201 gttatctgcg acccttaaac gtgactcttg ctgtatcact actcggccct cgatatgatg
     1261 ttcgcgagcc gccaccgctt gcgcgccgaa agcggcctgg tctcgatgat ccgcaccgtg
     1321 tcgccgacgc tcgcctcgtt gcgctcgtcg tgcactatga acttcttgga ccgacgcacc
     1381 cgcttcttgt acttcgggtg cctgaccagc gcctcgactg cgacggtcac cgtcttctca
     1441 gccttgtccg agacgatgat accgacgcgc tccttgcgcc tgtttcgctc ggtgtcggtc
     1501 tggatctggc cttcgtcctg atctagcgcc ggtcccttgg caggacccgc ttccaggccg
     1561 gcctcgacgg ttgcttcgcc cgactcgacg aggtcctggg gcgtttcggc cacgtcctcg
     1621 accgtctcag ggttctggtt tttctcttcc tctaccacta cccactctcc tgctgtttct
     1681 ggttcaaaac cgtcaggatc ctggcgatgt tcttcctgac ctctttgagc tgtcccgtgt
     1741 tctccaactg cccggttgcg tgctggaagc gcagattgaa gagctcgcgc cgcgtctcgg
     1801 cgaggcgccg ctccagttcc tggacgtcca gctctcgcac ttcggctacc ttcactgctg
     1861 accaccccct cgaaccatga agcgggtctt tatggggagc ttctgggcgg cgagatgcat
     1921 ggcttccctc gccagcgtct cgtccacgcc gctcatctcg aacatgacac gccccggctt
     1981 gacgacggcg acgtaagcct cggggctccc cttgccgcta cccatacggg tctcggccgg
     2041 cttcttggtc acaggcttgt gggggaagat gttgatcca
//
LOCUS       DATAIR010000333         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_332522, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000333 DATAIR010000000
VERSION     DATAIR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332522"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="hutU"
                     /locus_tag="VHM69_08190"
     CDS             <1..874
                     /gene="hutU"
                     /locus_tag="VHM69_08190"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2740410.1"
                     /translation="HDPLVGYVPAGYDLDAAAELRESDPERYISEARASMARHCAAMV
                     GFKERGSEVFDYGNSLRGEAKLGGFDGAYGYPGFVPAYIRPLFCEGKGPFRWAALSGE
                     ESDIAATDDAILELFPEDERLGRWISNAREKVHFQGLPARICWLGAGERHRAGLRFNE
                     LVADGTISAPIVIGRDHLDTGSVASPYRETEAMKDGSDAIADWPILNAMLNVAGGASW
                     VAVHHGGGVGIGRSIHAGAQVLADGTEDGAARLRRVLFNDPGIGVVRHADAGYESAQA
                     AARRLGIQMPALEG"
     gene            871..2124
                     /locus_tag="VHM69_08195"
     CDS             871..2124
                     /locus_tag="VHM69_08195"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX2740411.1"
                     /translation="MKRTLVPDLVLDAHEARNGLGVTIEDGRIVEVAPTTDGERLRGM
                     ALAPGFVNDHSHAFQRGLRGRVERIEPSHPPDDFWTWRERMYALAENLTPESMREVSR
                     RCYGEMLSAGYTSVTEFHYVHHRLDGTPYEDPNALAKAVALAAEDVGIRLLLLPVAYA
                     RGGVSRFRDASVDTFLERVDALRAWSEGRPLVEVGVAAHSVRAVPRAWLEEIGEYARR
                     EDLPIHTHADEQEREIEECQAEHGMRPVELLAQSGFLGPRTTIIHATHADGKELDLLA
                     EHGASVCACPTTEGNLGDGFLPAQGILKRDIRLSVGSDSHVRIDPFEELREIETNARR
                     LSGRRNVLVTKDDTSPTPWLLRAGWNRGGLETGDPADLIEIDLSHPALADVEAGDLTS
                     ALVFGAGSGIVAHTWVAGKRISEDV"
BASE COUNT          389 a          735 c          711 g          344 t
ORIGIN      
        1 ccacgacccg ctcgtcggct acgtccccgc aggctacgac ctcgacgccg cggccgaatt
       61 gcgcgagtcc gaccccgagc gctacatctc cgaggcacgc gcctcgatgg cccgccactg
      121 cgccgctatg gtcggtttca aggagcgtgg gtccgaggtc ttcgattacg gtaactcact
      181 gcgcggggaa gcgaagctcg gcggcttcga tggcgcttac ggctaccccg gtttcgtgcc
      241 tgcgtacatc aggccgctct tctgcgaggg caaaggaccg ttccggtggg cggcgctctc
      301 tggagaggaa tccgatatag ccgcgaccga cgatgcgatc ctcgaactct tccccgaaga
      361 cgagcgtctg gggcgttgga tctcgaacgc ccgcgagaag gtccatttcc agggtctccc
      421 cgcgcgcatc tgctggctcg gggccggcga acgccacagg gccggccttc gcttcaacga
      481 actggtcgcg gacggaacga tctccgctcc catagtcatc gggagggacc acctggacac
      541 gggcagcgtc gcctcgcctt accgcgaaac cgaggcgatg aaggacggct ccgacgccat
      601 cgcagactgg cccatcctta acgcgatgct caacgtagcc ggtggggcga gctgggtcgc
      661 cgtccatcac ggcggaggag tcgggatcgg acgctccatc cacgccggcg cccaggtcct
      721 cgcagatggc acagaagacg gcgcggcccg cctgcggcgc gtgctcttca acgaccccgg
      781 catcggcgtc gtccgccacg ccgacgccgg ctacgagtcg gcgcaggccg cagccaggcg
      841 cctgggcatc cagatgcccg cattagaggg ttgaagcgaa ctctcgtacc cgacctcgtc
      901 ctcgacgccc acgaggcgag gaacggactc ggtgtaacga tcgaggacgg acgaatagtc
      961 gaggtcgctc ccactacgga cggcgagcgt ctgcgcggaa tggcgctcgc tcccggcttc
     1021 gtcaacgacc acagccatgc cttccagcgc ggcctgcgag ggagggtcga gcggatcgaa
     1081 cccagccatc cccccgacga cttctggacg tggcgggagc ggatgtacgc gctggcagag
     1141 aaccttaccc ccgagtcgat gcgcgaggtc agccgccgct gctacgggga gatgctctcc
     1201 gccggctaca cgagcgtcac cgagttccac tacgtccacc accgtcttga cggaacacca
     1261 tacgaagacc caaacgccct tgcgaaggcc gtcgccctgg ccgctgaaga tgttggcatt
     1321 cggctcctgc tgctccctgt cgcatacgcg aggggcggtg tctcgcgctt ccgcgacgcg
     1381 tccgtagata cgttcctgga gagagtcgat gcgcttcggg cgtggtcgga agggcgtccg
     1441 ctggtcgagg tcggcgtcgc ggcccacagc gtcagggcgg ttccacgagc atggcttgag
     1501 gagatcggag agtatgcccg tcgcgaagac ctgccgatac acactcacgc cgacgagcaa
     1561 gagcgtgaga tcgaggagtg ccaggcggag cacgggatgc ggcccgtcga gttgctcgcg
     1621 cagtccggct tcctcggccc acgcaccacg ataatccacg ccacccacgc tgatggtaag
     1681 gagctggacc tcctcgcgga acacggggcc tcggtgtgcg cgtgccccac gacggagggg
     1741 aacctgggtg acgggttcct gccggcgcag ggtatcttga agcgcgacat cagactctcc
     1801 gtcggctccg actcgcacgt acgcatagac cctttcgagg agctgcggga gatcgagacg
     1861 aacgcccgca ggctctccgg acgcaggaac gtcctcgtca cgaaggacga cacctcacca
     1921 acgccgtggc tgttgcgggc tggatggaac cgtggcggat tggagaccgg cgacccggcg
     1981 gatctcatcg agatagacct ctctcatccg gcgctcgcag atgtggaagc cggtgacctt
     2041 acatccgcgc tcgtcttcgg cgcaggtagc ggaatcgttg cccatacgtg ggtagcgggc
     2101 aaacgcatct ccgaggacgt gtgaggacac ccgactcatg aaccaacgcg tttcccgtaa
     2161 cgaccggcct ccactacgc
//
LOCUS       DATAIR010000334         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_298779, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000334 DATAIR010000000
VERSION     DATAIR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298779"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHM69_08200"
     CDS             complement(<1..573)
                     /locus_tag="VHM69_08200"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX2740412.1"
                     /translation="MEEHEDRGLAERVEALEEQVRMQGRDLRFAIQRLESMTAGQTAE
                     ERHGAGRESWREERLEEPEEAGRSSRTRRLGVPSGLKNLPHGEWWLSKIGIGLLLFGA
                     AFLFKFSVDQNWITPSVRVGIGFALGALLVLLGLRVYGERRAFSQVLLGGGVGVFYIT
                     GYAAFQLYALVPYAAAFAFMVAVTLFAFFLS"
     gene            complement(778..2358)
                     /locus_tag="VHM69_08205"
     CDS             complement(778..2358)
                     /locus_tag="VHM69_08205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2740413.1"
                     /translation="MSEVRERTMEELELGVFDVLVLGGGIVGGRVAFDAARAGLRVAL
                     VDAGDFGGATSGASARLVHGGLRYLRTGNVRLVRTALRERNVLASRIAPHLVRPLPFV
                     LSAAEGQRSKCAAGLIAYAALNGFGRPLPRFITPEEAVRLVPSLRVDGLTSHAVFQEA
                     ETNDSRLALATVAAAVRSGAVAANYLRAVALELAPGKNSRVLLQGREGTLIVRCRAVV
                     NATGPWVDLVRKMEDPGCGPVARLSKGIHVVLRPAEKWRAAVAVSMDGGRHLYAVPCE
                     DMILLGTTDEEYIGDPAGVAAEPADVVSLLEMAQHFLRPEMLDGERMVSAFAGLRVLS
                     RGEVASRRASRDHLLSVGPGGMVSVAGGKLTTHRQIALDAMRRLPSHVRPSRLSLSDA
                     PLPGASPICRRELYSRLDRSTVDHLLHHYGAESARLLEYSKVHHDALEQITPGAPDLW
                     AQVHHAISEEWAVTAEDIIYRRTTLGPRGLDTPEIRQRISRLLEAEIPTPQSTSPPAS
                     AHTFHVSPRRSNSPQVRR"
     gene            complement(2355..>2904)
                     /locus_tag="VHM69_08210"
     CDS             complement(2355..>2904)
                     /locus_tag="VHM69_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740414.1"
                     /translation="PYGLFVLACTRLGHDLDALVNGVVRAFPPGEPVEMLFRRLRRRP
                     STPLLAMLSRRLGTFDEVRLARRASAGERAARRLRASVHPGRKSLQPTHWLFPVVAPD
                     PGALISGLRRRGFDASRVTSSISVVEAPAGCSSAAEASRMMSGIVFLPVCQDLPSQAS
                     DAMVDLVNECTVAEPVEDLARP"
BASE COUNT          556 a          954 c          892 g          502 t
ORIGIN      
        1 cgacagaaag aaggcgaaca gcgtcaccgc aaccatgaac gcgaaggccg ccgcgtacgg
       61 cacgagcgcg taaagctgga aggccgcata gcccgtgatg tagaagaccc cgaccccacc
      121 cccgagaagc acctggctga aggcccgacg ttcaccgtac acccgtaatc cgagaagcac
      181 gagcaacgcg ccgagtgcga acccgatccc aacacgcact gaaggtgtga tccagttctg
      241 atccaccgag aacttgaaca ggaacgccgc cccgaacaac aataacccaa tgccgatctt
      301 actgagccac cactccccat gtggaagatt cttaagcccg gacggcaccc cgagccgccg
      361 cgtcctgctc gacctcccgg cctcttccgg ttcttcaagc ctttcctccc gccaactctc
      421 ccgcccagcc ccatgccttt cttccgcagt ttgtccggcc gtcatactct ctagccgctg
      481 tatcgcgaac cgcaaatcac gcccctgcat cctgacctgc tcttcgagcg cctctacgcg
      541 ttctgcgaga cctcgatcct catgctcttc catgatactg tcctctttcg ataaccgatg
      601 ccccactttg cataacccac tccacagacc cgagttcctc caccgacttc cggcaccgat
      661 gcaccactcg gaaccataaa ctcaggagat tccatttcgt aattgcccgg ttttccatcg
      721 tgtcatcgtg ccccatgtcg tacttacgtt gtaagttaac atattgcggc gtgctagtca
      781 acgacgaacc tgaggtgagt tcgaccgtcg gggagaaacg tggaacgtgt gtgcggacgc
      841 tggcggactc gtggactgtg gggttgggat ttcagcttcg aggaggcgtg aaatgcgctg
      901 gcgtatctct ggagtatcga ggccacgcgg accgagggtg gtccgacggt agatgatgtc
      961 ctctgcggtt acggcccact cttcggagat cgcgtggtgg acctgcgccc agagatcagg
     1021 cgctcctggg gttatctgtt cgagggcgtc gtggtgcact ttgctgtatt cgaggagcct
     1081 ggcgctttct gcgccgtagt ggtggaggag gtgatccacg gtcgatctgt cgagacggga
     1141 ataaagttct cgtcggcata tcggggaggc gccggggaga ggtgcgtcgc tcagggatag
     1201 tctgctgggc cgaacgtgtg acgggaggcg tcgcatggcg tccagggcga tctgtcggtg
     1261 cgtggtcagt ttgccacctg ccaccgagac catgccgccg ggcccgacgc tgaggagatg
     1321 atcccggctc gcgcgacgcg acgctacctc gcccctcgat agtacgcgca ggcctgcgaa
     1381 agcggagacc atgcgttcgc cgtcgagcat ctcagggcgc aggaagtgtt gggccatttc
     1441 gagaagggag acgacgtcgg cgggctccgc ggccacgccg gcggggtccc cgatgtactc
     1501 ttcatcggtc gtgccaagca ggatcatgtc ctcgcaggga acggcgtaga ggtggcgtcc
     1561 gccgtccatg gagacggcga ctgcggcccg ccacttctcg gcgggcctga gtaccacgtg
     1621 aatccctttg ctcaggcggg cgacgggccc gcagccggga tcttccatct tccggacgag
     1681 atccacccac ggaccggtcg cgttgacgac ggccctgcat ctgacgatga gagtgccttc
     1741 gcgtccctgc agcaacacgc gtgagttctt tcctggtgca agttccaaag ccacagcccg
     1801 cagataattt gctgctacag cgccagagcg cacagccgcg gccacggtgg cgagcgccag
     1861 gcggctatcg ttggtctctg cctcctgaaa gacggcgtgc gaagtgagac cgtcgacgcg
     1921 gagcgaaggc acaagacgaa cagcctcctc aggagtgatg aagcggggaa gcggtctgcc
     1981 gaagccgttg agcgcggcat aggcgatgag ccccgccgcg cacttcgagc gctgtccttc
     2041 tgccgctgac agcacaaaag gcagcggcct gaccaggtgc ggtgcgatac gcgaggcgag
     2101 tacgttgcgc tccctcagag cggtacgcac gagccggaca ttgcccgttc ggaggtagcg
     2161 gagcccaccg tgcaccagcc gcgcggaggc ccccgaggtc gccccaccga agtcccctgc
     2221 gtcgacgagc gccaccctga gaccggcccg cgcagcgtcg aatgccacac ggcccccgac
     2281 tatgccgccg cccaacacga gcacgtcgaa cactccgagt tccagttcct ccatcgtgcg
     2341 ttcccgaacc tcgctcatgg ccgcgccaga tcctcgaccg gttcagccac ggtacattcg
     2401 ttgacgagat cgaccatggc atcggaggct tgtgaaggga gatcctggca tacaggcagg
     2461 aagacgattc ctgacatcat ccgtgaagct tcggccgctg acgaacaccc ggcgggagct
     2521 tcgacgacgg agatgctgct cgtaacccgg ctcgcgtcga agcctcgcct tcggaggccc
     2581 gagatcaaag ccccagggtc aggagcgacg accgggaaga gccagtgcgt cggctgtaat
     2641 gattttctcc ccggatgcac actggcccgc agtctacgcg ccgcacgctc gccggcagag
     2701 gccctcctgg ctagcctgac ctcatcgaac gtcccaagcc gccgggagag catcgcgagc
     2761 agcggcgtgg aaggcctgcg tctcaggcgc cgaaagagca tctcgaccgg ctccccgggc
     2821 ggaaaggctc ggaccacgcc gttgaccagg gcgtccaggt cgtggccgag ccgcgtgcat
     2881 gccaggacga acagcccgta gggt
//
LOCUS       DATAIR010000335         8311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_436777, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000335 DATAIR010000000
VERSION     DATAIR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436777"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHM69_08215"
     CDS             <1..244
                     /locus_tag="VHM69_08215"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX2740415.1"
                     /translation="AGLRLDRAALVRAGMATLFLVVGKLFLVDLAGVEAVWRILLFLG
                     FGGLFLVLGYYLQHLWKPGPPDTFDGNDSLSSGRGA"
     gene            335..1849
                     /locus_tag="VHM69_08220"
     CDS             335..1849
                     /locus_tag="VHM69_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX2740416.1"
                     /translation="MGERTRLGLGILGGALALGGLGDALLRATPWGVNFLLWITALVG
                     LSLLLVMWGRVGTGGEGRWLVFVALLFAFGVVLRDSTVVVFLDVVAVLISLSLAVWRG
                     HSGSLRRAGLSEYVLGGAYAGSLSSAGPIPVSVSDVDWREVVRGRWKGQALAVSRGVL
                     LAAPLLLLFGALFVAADAIFERLVLDVFGFDLAEIFQHLLLISVFAWITAGMFWTGLM
                     ARAPENLVIRRPRIISLGIVEVGIVLGLLDLLFLAFVAIQVRYLFGGAERVAATAGLT
                     YAEYARRGFFELVTVTALTLPLLLLSHWLLRRENRAHERVFMALAAIMVGLLFVVVAS
                     ALQRMYLYQQAFGLTELRLYTTVFMAWISAVLLWFVFTVLRNRRDSFAFGALVAGFVA
                     ILAINAMNPDAIIARTNVERAENGRELDVYYLTTLSADAVPVLVKAFPEIGDKRFQDR
                     WTGERHTFRGEITDRWNAKNSDWRTWNLGRSRARLSVRTYLEEQAGRMPTMARR"
     gene            2098..2400
                     /locus_tag="VHM69_08225"
     CDS             2098..2400
                     /locus_tag="VHM69_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX2740417.1"
                     /translation="MLTEERVQDQLRNVIDPEIGMDLVELGLIYDVGVHDEGRHVDVT
                     FSLTSPMCPVGDLIQEQVETETLSIEGVETVNAQLTFDPMWNPEMMSPAAKLFFGR"
     gene            2958..3593
                     /locus_tag="VHM69_08230"
     CDS             2958..3593
                     /locus_tag="VHM69_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740418.1"
                     /translation="MDRRSVRTWLRKVDQSTSGARETFSPIVKGMGDEELVGQVAQGD
                     ERALSELYDRYSRPMYATGVRLLGDSQLAEELVQDAFTNVWRGAASFDPNRASFATWL
                     YRLTRNRAVDLNRRRRVRPLSSGEEPLRNVSGGPEPEASVDLWDVAGALSLLSAEHRE
                     VLNLAYFEGLSQREISQRTGVPLGTIKSRTTAALKRLRRAMEVDSVGGAST"
     gene            3590..4261
                     /locus_tag="VHM69_08235"
     CDS             3590..4261
                     /locus_tag="VHM69_08235"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2740419.1"
                     /translation="MSEDRFEELLGPYLLRQLTAEEEQELEHHLEECSYCRDELERVR
                     QTHTLLRELAASVPPAELKARTMARARGESTTRSGVRWKVWVPAAAALLLAAILGIGF
                     FQAIVGDSSSGVPLTATDLAPDASGELLGEKVGENIQFELEVRGLPKLRKDEYYEMWY
                     AKENGERISCGTFRAQPGGRTAVNLTAPVNAASYPIIEVTREPDDGDPGTSGKEVLVG
                     DLRSL"
     gene            complement(4685..5233)
                     /locus_tag="VHM69_08240"
     CDS             complement(4685..5233)
                     /locus_tag="VHM69_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740420.1"
                     /translation="MTHLCDAELRDRVDEYRNPDMEAQAVPHGVILLVEDDARLAELT
                     TAFLLKGEGGPNEVVVASDGVEAIDYLHDPGRGASEMPHLVLLDLNMPRLDGFGVLKK
                     MRAEERTMFVPVVMLTSSDRSVDVRTAYSLGANGYLDKMPSRVSWEEMVQTVARFWVV
                     MNMTPYSLAGQDNGFARRTRPS"
     gene            5855..6031
                     /locus_tag="VHM69_08245"
     CDS             5855..6031
                     /locus_tag="VHM69_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740421.1"
                     /translation="MVDLSVDNEAALAWLDGALNEAIARGQMALIGYLEAVLEDLEFE
                     MELGAPPGHLFGVS"
     gene            6272..7009
                     /locus_tag="VHM69_08250"
     CDS             6272..7009
                     /locus_tag="VHM69_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2740422.1"
                     /translation="MWSELRGYWASVDRTPIKVLLADDHTMFREGLANLLTAYGGLEV
                     VAQTTNDDGAVALAREKKPDVVIMQVQMPFAKAKEALDRMRAIQPAPKVIIVTMFEDP
                     AFMREFLRLGASGYMLKSSSSTQLIGAIRVATFAPEENNVVIGLPRQLLEEAEGGSEG
                     VLSVRELEILLLAARGLSNRQIATRVRLAEGTVKRHLTNTYHKMGVGSRGEAARKALQ
                     EEWITLSEITDEDLIEDATPEDGQERE"
     gene            complement(7026..7373)
                     /locus_tag="VHM69_08255"
     CDS             complement(7026..7373)
                     /locus_tag="VHM69_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740423.1"
                     /translation="MRILVNAKPKMYGEAIALALHKYRPEAEVMLISAESLDGEIGSF
                     RPHLILHNDNVRIAPEELERVVCRIEILFSDGMDARIIMSGQAVSKEDMSLEEMFSVV
                     DEVEKLVLGETPG"
     gene            7860..8054
                     /locus_tag="VHM69_08260"
     CDS             7860..8054
                     /locus_tag="VHM69_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740424.1"
                     /translation="MRAAGLSRSKVSYLRDLAGHVIHGDLEALDTLPDEEVIDQIIDV
                     KGLGRWSADMFKSITAAYAD"
BASE COUNT         1635 a         2423 c         2575 g         1678 t
ORIGIN      
        1 cgccggtctt cggctcgacc gggccgccct tgtgcgggcg ggcatggcta cgctcttcct
       61 cgtggtcggc aagctcttcc tcgtcgacct cgccggggtc gaggcggtgt ggcggatact
      121 gcttttcctg gggttcggcg ggctcttcct cgtgctcggc tactacctgc agcacctctg
      181 gaagcccgga ccgcctgaca cattcgacgg taacgactcg ttatcttcgg ggcgaggagc
      241 ttagaaaagg ttagtccttc ttaaccggcc gttgccctgg ctgttacgtc cgtccgctac
      301 agtgcccgtc aggctatgac gggaggcacc ttctgtggga gagaggacga ggctcgggct
      361 cggtatcctc ggcggcgcgt tggcgctcgg cggcctggga gacgccttgc tgcgcgcaac
      421 gccttggggg gtcaacttcc tactctggat caccgctctg gtcggcctct cgctgttgct
      481 cgtgatgtgg ggtcgagttg gcacaggggg agaggggagg tggcttgtct tcgtggcgct
      541 cctcttcgcc ttcggggtgg tgttgcgcga ctctaccgtg gtggtgtttc tcgatgttgt
      601 ggcagtcctg atctccctct ccctcgcagt gtggcgtgga cacagcggga gcctgcgccg
      661 cgccggtctc tctgagtacg tccttggcgg ggcctatgcc ggctccctca gctccgcggg
      721 accgatacct gtgagcgttt ccgacgtaga ttggcgcgag gtcgtccggg gacggtggaa
      781 gggacaggcg ctcgcggtat cgcgcggcgt gctgctcgcg gcgccgcttc tgcttctctt
      841 cggcgcgctc ttcgtggctg ccgacgccat cttcgagcgg ctcgtgctcg acgtcttcgg
      901 cttcgacctc gccgaaatct tccagcatct cttgcttatc tccgttttcg catggatcac
      961 cgcgggaatg ttctggacgg ggctcatggc tcgcgcccct gagaacctcg tgatccgtcg
     1021 tccacgaatc atctccctcg gcatcgtgga ggtcgggatc gtgctcggtc tcctcgacct
     1081 cctcttcctt gcgttcgtgg cgatccaggt ccgctacctg ttcggcggcg cagaacgcgt
     1141 cgccgccaca gcaggcctga cctacgccga gtacgcccgt cgcgggttct tcgaactcgt
     1201 caccgtaacc gccctcacgc ttcctctgtt actgctctcc cactggctcc tccgcagaga
     1261 aaaccgcgcg cacgagcgcg tgttcatggc gctggccgcg atcatggtcg gattgctgtt
     1321 cgtcgttgtc gcctccgcgc tgcagcggat gtacctgtat cagcaggcgt tcggcctgac
     1381 cgagctgcgt ctgtacacca ccgtgttcat ggcctggatc tccgccgtgc tcctctggtt
     1441 cgtcttcacg gtgctaagga accgtcgcga cagcttcgct ttcggcgccc tggtggccgg
     1501 cttcgtcgcc atcctcgcga taaacgccat gaaccccgac gccatcatcg cccgtaccaa
     1561 tgtggagcgg gcggagaatg gacgcgaact cgacgtctac tatctcacca ccctaagcgc
     1621 cgacgccgtg cccgtgctcg tcaaagcgtt ccccgagata ggagacaagc gatttcagga
     1681 tcgatggact ggcgagagac acacgttcag aggcgagatc acagaccgct ggaacgcgaa
     1741 aaactccgac tggcgtacct ggaaccttgg ccgttcgaga gcccgcctgt ccgttcgtac
     1801 ctacctcgaa gagcaggccg gacgaatgcc aacgatggcc cgacgttaaa tgctggtaac
     1861 aagatagggc gctcatgcac cgccatcgct gagttatcca caatatgttg tggataagga
     1921 gcgtagaacg gtggataact gcatcagttg taacaagcct gtaacagaag agcggcggct
     1981 cgttccatgc cgcgatgcgc cgaaatgctc tgctttcgct gcgcgtgcgc gggtggaaaa
     2041 gcgtcatgtg ggcattacaa tgagtctgcc gaacccacgc caacaagagg tgaaaaagtg
     2101 ttaaccgagg agcgcgtgca ggaccagttg cggaacgtca tagaccctga gatagggatg
     2161 gacctcgtgg agttgggtct gatctacgac gtgggcgtgc atgacgaggg ccgtcacgtg
     2221 gacgtcacct tcagcctcac cagcccgatg tgcccggttg gggacctgat ccaggaacag
     2281 gtcgagacgg agaccctctc catcgagggc gtcgagaccg tgaacgccca gctcacgttc
     2341 gatccgatgt ggaaccccga gatgatgagc cccgccgcca agcttttctt cggccggtaa
     2401 tccggacata gaatcgacga taccgtcccg gtgatatcca aagcggagac accgggacgc
     2461 ttcatcttct aatgcgcgtc tcaaacgcac gtcctccacc cttcccagac gacccgtcgc
     2521 cgccatgcta cgtcgtccac ggcctgtctc agagtgggcg atccctcaag gaagatgcga
     2581 gttcgccacc gagacaaatg tcaatcaact gaaagcgagg ccgcaggccg aagcgagctg
     2641 aaaggcgcga agcgccgtgc tgacaagcga aggcgaagcc tgagcgtgct aaaagcgaag
     2701 cccgcagggc gtagcgtgct gataggcgcg acatcgttcg ggtcaccgtt ggagagttcc
     2761 tcgttacacg tttcggacga aaaccaatta gatgctgcgc tgcgtacaaa ttgggtgtag
     2821 gagcactttg acaacgacgt acaggttcga ggtcgggtaa taatggtcat tggcggggat
     2881 ccggtggagg aggtcgtggg gcctctatca ttggtcccga agtgaccctg ccgaatagcg
     2941 ctctacgtat ataaacgatg gaccggagaa gtgtccgtac gtggcttcgc aaggtagacc
     3001 agagcacctc cggcgcgagg gagacgttca gtccgatagt gaagggtatg ggggacgagg
     3061 aattggtagg ccaggtagcg caaggggacg aacgagccct ctcggagcta tacgatcgct
     3121 actcgcgccc gatgtatgcc acgggcgtca ggctcctcgg ggattcccag ctggccgagg
     3181 agctagtcca ggacgctttc accaacgtct ggcgaggggc ggcatcgttc gatccgaacc
     3241 gtgcgagctt cgcaacctgg ctctacaggc taacccgcaa ccgcgccgtg gacctgaacc
     3301 ggagacggcg ggtgaggcct ttgtcgagcg gggaggaacc acttcggaac gtctccggag
     3361 gcccggagcc cgaggcgagc gtggaccttt gggacgtggc cggggcgctt tctctcctct
     3421 ccgcggagca cagggaagtg ctgaacctgg catacttcga gggcctttct cagcgtgaga
     3481 tctcccaaag aaccggcgtc ccattgggga cgatcaaaag ccgtaccacg gcggctttga
     3541 aaaggctgcg ccgtgccatg gaagtcgact cagttggggg agcctcgacg tgagtgagga
     3601 ccgcttcgag gagcttcttg gcccgtacct tttgcgccag ttgaccgcgg aggaggagca
     3661 agagctggag caccatctgg aggaatgttc ctactgccgg gacgaactcg agcgtgtccg
     3721 ccaaacccac actcttcttc gagaactggc cgccagcgtg cctccggcag aactcaaggc
     3781 ccgaacgatg gcgcgggccc gaggtgagag cacgacacga tccggagtca ggtggaaggt
     3841 atgggtgcct gccgctgccg cgttgctgct tgccgcgatt ttgggcatcg ggttcttcca
     3901 ggcgatagtc ggcgactcct cgtccggggt gccgctgact gcaacggatc tggccccgga
     3961 tgccagcggc gagcttctag gagagaaggt aggagagaac atccagttcg agctggaagt
     4021 gcgggggctg ccaaaactcc gcaaggacga atactacgag atgtggtacg ccaaggagaa
     4081 cggggagagg atcagctgcg gcaccttccg tgcgcagcca ggaggccgga ctgctgtaaa
     4141 cctcacggct ccagtcaacg ccgcttcgta cccgataatc gaggtgaccc gcgagcccga
     4201 cgatggagac cccgggacga gtggtaagga agtcctggtc ggagaccttc gaagtctttg
     4261 agcttatacg ctcggaagac gttggtaccg gagagtagtg ccgggacaga agggacatat
     4321 gaacagcgtc gagtaaccgg agggataggt ctacgcgcta ctttctggct ggcctggtcg
     4381 ctcgccggtc tcactgtggt catgatcgtg gccggcattg cgctctgctt cctcgctcgg
     4441 gccgtccacg ttgcaggcag ttggggagcg aatctcacca taagtaagct gttggggggc
     4501 ttctattctt ggccttccca ctagtgggtg cgctgatctc ctccaggcgt ccccgaaacc
     4561 ctatttgctg actctgtgga cacgcagcgg aatgctcgac tactacgaat ggaacgcttc
     4621 cacgtctcgc cctcacctcc acagtgggcc ggcagccggg ggcgaactgg agatgaattt
     4681 gccgctacga aggtcgggtc cggcgtgcga acccgttatc ctgtcctgct aacgagtacg
     4741 gagtcatgtt catcaccacc cagaagcgcg ccaccgtttg caccatctct tcccacgaca
     4801 cacggctcgg catcttgtcg aggtagccgt tggctcccag actataggcc gtacgtacgt
     4861 cgacagagcg gtcggaggat gtgagcatca ctacgggtac gaacatggtc cgctcctcag
     4921 cccgcatctt ttttagcacc ccaaagccgt ccagccgggg catgttgagg tccaggagca
     4981 ccaggtgtgg catctcagaa gcgcctcgcc cagggtcgtg gaggtagtcg attgcctcta
     5041 cgccgtcgct ggcgacgacc acctcgtttg ggcctccttc ccccttcagc aggaaagcag
     5101 tggtcaactc cgcgagccgt gcgtcgtcct ccaccagcaa aatgactccg tgcggcacag
     5161 cctgggcctc catgtcggga ttgcggtatt catctactct atctcgaagc tcagcgtcac
     5221 agagatgggt caactacatt gaataccgcg ccagttactc ttggcatcat ccaagatcta
     5281 aggtgcaggg actaccatta tggctattct cctgcgcgct tacgtgcggt gatagtaacg
     5341 tgtgcgacac gctctgtccc cgcctacggc ctggatggcg ccgaaaaagt ctcgaagtgt
     5401 gtagtgaggc tacgcgagcc tcccttctat gtccaagttt gtcctgaggg ctttccgcga
     5461 gatgggcatc tcggacagaa tgcctgggtt ttcgcgttgt gcctatctcc ggatgggtca
     5521 gggcttgttc cacggtggag aagctaaggt cgcattaagc gtcgtgaagg atgtttgacg
     5581 aagccttgcg agaaccacgc cagctggtag ggtcggggga aggagcttgg tgccctctct
     5641 gggtgatagg cgggaaaggg aaaggagaag tgggcaccaa gttacagtct tactaacaca
     5701 cctgaccgat ccatcaagct gttatcgtcg ctgaccagcc gaaaatgtat cgaagtatgt
     5761 agcccgtacg caatggcgtg tgcctacact gctttacaat gcgaacaccg tagtccatgg
     5821 ggagttcaac ggtgctacgg aaaggagcac gaaggtggtt gatctgagtg tagacaacga
     5881 ggctgctttg gcgtggttgg acggtgcgtt aaacgaggct atcgcccgag gccagatggc
     5941 actcataggc tacttagaag cggttctgga agatctggag ttcgagatgg agctgggtgc
     6001 tcctccagga catttattcg gagtatcgta ggcttgggcg aacgattggt gggcctcctg
     6061 cgggggtgga ttgcccacgt cgtctcggcc tacccttacg gccgcgtccg ccgggtgctt
     6121 acctgaagcc ggccgctcac tccaggtagg tgcgtggtcg gaagttggcg ttgagcgcta
     6181 acttcatggt gtcccagcgc tctgtcgctt ccatcgggta cacgtgcgta ttagaatgta
     6241 gcgatcatag ctcgatcaca agccgagagg catgtggtcc gaactcaggg gatattgggc
     6301 ttccgtggac agaactccta taaaagttct tcttgcagac gatcacacca tgttccggga
     6361 gggactggcg aacctactga ccgcttacgg aggactggag gtggttgctc agacgaccaa
     6421 tgacgatggg gccgtagctc tggcccgcga gaagaagccc gacgtggtga tcatgcaggt
     6481 acagatgccc ttcgcgaagg ccaaggaagc actcgatagg atgcgcgcca tccagcctgc
     6541 gccgaaggtg atcatagtca ccatgttcga ggaccccgca tttatgcgcg agttcctgag
     6601 gctgggagca agcggctaca tgctaaaaag ctcgtcctcc acccaactca tcggcgccat
     6661 tcgcgtcgcg accttcgctc ccgaggagaa taacgtggtc ataggtctgc cgcgccagtt
     6721 gctagaggag gccgaaggag ggtccgaggg tgtgctctcc gtccgggagc tcgagatact
     6781 gcttctggcc gcccgtggtc tgtcgaaccg tcagatagcc acgagggtgc gtcttgccga
     6841 gggaactgtc aagcgtcatc tcaccaacac ctaccacaag atgggcgtcg gctccagggg
     6901 agaggccgcg agaaaagccc tgcaggagga gtggatcacc ctcagcgaga tcaccgacga
     6961 agatctcatc gaggacgcaa ctcccgagga cggacaagaa agagagtaac cgggaggcgt
     7021 caggatcacc caggcgtttc tcccaggact agcttctcca cttcgtcaac gaccgagaac
     7081 atctcctcca gactcatgtc ctctttactc acggcctgcc cgctcatgat gatcctggca
     7141 tccataccgt cgctgaacag tatctcgatc ctgcacacca cgcgttccag ctcctcagga
     7201 gcgatcctaa cgttgtcgtt gtgcaggata aggtgtggcc tgaagcttcc tatctctccg
     7261 tcgagagact cagcggagat gagcatcacc tcggcctcgg gccggtattt atggagggcg
     7321 agggcgatcg cctcaccata cattttgggc ttcgcattaa ccagtatacg cacgatccgt
     7381 acgctccttc ttgcaggaaa ctcgacacgg gacgaaacgc atgaaagcag cccacaaata
     7441 gattatgaaa cgcactatac tcccgatgac attggattgg agtggtacgg accgatgatg
     7501 ggctaaagtg tgtagtgtat ctcctcttca agataggttt ggcgcccgct ccaaagcgta
     7561 ctccgcgggg gaagcctgaa tccggatctg gaggaggttg ggaaaatcga tgcaggaaga
     7621 acgcacctcc ttgcctgcgg cgaccccgtg atggcaactc tcgtccgcga cgccggaccg
     7681 ctgggcctgg aggagcgcca tcgcggacga cccgccgacg cctacggagc cctggtgcgt
     7741 accatcgtcg gccagcaact ctccacgaag gcggcgcgca ctatctacac ccgcccttgc
     7801 gcgtttttag gtgaccgtcc tccccgccgg aggagatcct cgccgctgac gaggaggcgt
     7861 tgcgtgcggc cgggctctcg cggtccaagg tctcgtacct ccgcgacctc gcagggcacg
     7921 tgatccacgg cgacctcgaa gcactagata cccttcctga cgaggaggtg atcgaccaga
     7981 tcatcgacgt caagggactc gggcgctgga gcgctgacat gttcaagtcg atcacagcgg
     8041 catatgctga ttgagatcac cttgtctgcg cagggtatag aggtgcgctg agagacacga
     8101 gaatcgccca caagcgccgc ggaggagcca actagagcgg tttgcggaaa gagtgaacct
     8161 gaggtgagta aaggtttcag gcatcaggca tcagcaaaaa gacctgatgc ctgtagcctt
     8221 ctccgaagga gtagctcatc cttttcgaga accgccatag aggatgggtg atcattgagc
     8281 tcaagccccg gaggaaggtc tgttcctata t
//
LOCUS       DATAIR010000336         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_21723, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000336 DATAIR010000000
VERSION     DATAIR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21723"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VHM69_08265"
     CDS             <1..642
                     /locus_tag="VHM69_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740425.1"
                     /translation="AFAREGARVVVNARREESIRPVAEEIEGTGAEVLTVAGDVSKGA
                     DVERMVHETVERFGKIDVLVNNAGLLGPRVRIEEYPEEEWRKVIDANLTGAFLVSKAA
                     IPHLPEGASIINVVSGVSVEGRAEWGAYSVSKFGVEGLTQILAAELAGRAVRVNAVDP
                     GGMRTDMRAAAYPEEDPATRITPEENTAVFIYLASDESRDVTEERFKAQEFGD"
     gene            complement(650..1306)
                     /locus_tag="VHM69_08270"
     CDS             complement(650..1306)
                     /locus_tag="VHM69_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2740426.1"
                     /translation="MRKFDVKRSRARNIISWVLSLVMIAAGIALIASFFLAGRLDSTA
                     TNSDDPGGFNVPRLESTKDENTSSAGPKDKTLELTVPAMARIENDAIPYTTGTDEEAL
                     KSHAAIHLEGTGFPWDSEANVYIAGHRLGYPNTESWLTFWDLNEVGAGDKVYVTDAND
                     TKYTYKVFKSFVVSPSDTHVTDTLPGKNILTLQSCTLPDYKQRLIVQAELTNTSEKAA
                     "
     gene            complement(1366..>1878)
                     /locus_tag="VHM69_08275"
     CDS             complement(1366..>1878)
                     /locus_tag="VHM69_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2740427.1"
                     /translation="LNYYGHERVSVLDGGWRKWTSEGRPTTVEVPDPVPASFEPNLRA
                     GWRTDAETLLAGGAFVLDARDEGQYTGAVARGEGRAGRVPRAAHLHSDSLFDPESGTF
                     HSGEELARRVREAGVPEDRDEPVVAYCNGGVAATVPLFTLHRLGYKNLANYDGSWNEW
                     GLRDDLPVER"
BASE COUNT          322 a          564 c          643 g          349 t
ORIGIN      
        1 gccttcgcga gagagggcgc gcgcgtcgtc gttaatgccc gcagggaaga gtccatacgc
       61 cctgtcgcgg aggagatcga agggacaggc gcggaggtcc tcaccgtcgc cggtgacgtg
      121 tcgaagggcg cggacgtgga gaggatggtc catgagacgg tcgagcgttt cgggaagata
      181 gacgtgctcg tcaacaacgc cgggctactc gggccccgtg tcaggataga agagtacccc
      241 gaggaagagt ggagaaaggt catagacgcc aacctcaccg gggctttcct cgtctcgaag
      301 gcggcgatcc cgcacttgcc ggagggtgct tccataatca atgtcgtgag cggggtcagc
      361 gtcgagggac gggcggagtg gggagcatac tcggtcagca agttcggcgt cgaagggctg
      421 acccagatcc ttgccgccga gctcgcgggg cgcgccgtcc gcgtgaacgc cgtggacccg
      481 ggcgggatgc gcacggacat gcgcgccgcc gcctaccccg aagaggaccc cgccacgcgc
      541 ataacccccg aggagaacac agccgtcttc atctatctgg catcggacga gtcgagggac
      601 gtaacggagg agcgcttcaa ggcgcaggag ttcggggact gagaggctgc tacgccgcct
      661 tctccgaggt gttggtcagc tcggcctgga cgatcagacg ctgcttgtag tcgggcagcg
      721 tgcatgactg gagggtcagg atgttcttgc cgggtagggt atccgttacg tgggtgtccg
      781 aagggctgac gacgaaggac ttgaagacct tgtaggtata tttcgtatcg ttcgcgtcgg
      841 tgacgtagac cttgtccccg gcaccgacct cgttcaggtc ccagaacgtc agccagctct
      901 cggtgttggg gtagcccagg cggtggccag cgatgtagac gttggcctcg ctgtcccagg
      961 ggaagccagt gccttcgagg tggatggcgg cgtgcgactt tagcgcctct tcgtcggtac
     1021 ccgttgtgta cggaatggcg tcgttctcga tgcgggccat cgcggggacg gtgagctcga
     1081 gggtcttgtc cttggggccc gcgctgctcg tattctcgtc cttggtactc tcgagcctgg
     1141 gcacgttgaa gccgccaggg tcgtcgctgt tcgtggctgt gctgtccagg cgtccggcca
     1201 ggaagaagga ggctatgagc gcgatgccgg ccgcgatcat gacaaggctc aacacccatg
     1261 aaatgatgtt tctggccctg gatctcttga cgtcgaactt cctcatgaac tcggagattg
     1321 taacatcgaa ggtgctcctt tatcggtgga gggaccagaa catgctcaac gctctacggg
     1381 caggtcgtcc ctgagacccc actcgttcca cgaaccgtcg tagtttgcca ggttcttgta
     1441 accgagcctg tgcagcgtga agagcggaac ggtagccgcg accccgccgt tgcagtaggc
     1501 gacgaccggc tcatccctat cctcgggcac gccagcctcc ctcaccctcc gcgccagctc
     1561 ctcccctgaa tgaaaagtgc cgctctcggg gtcgaagagg ctgtcggagt ggaggtgggc
     1621 ggcacgcggc acgcgccctg ctctgccctc cccgcgcgcc acggctcctg tgtactgccc
     1681 ctcgtcgcgg gcgtcgagca cgaaggctcc accggcgagg agcgtttcgg cgtccgtccg
     1741 ccagccggct ctaaggttcg gctcgaagga ggcgggtacg gggtcaggaa cctcgaccgt
     1801 cgtcgggcgg ccctccgagg tccacttcct ccagccgccg tccagaacgg agacgcgctc
     1861 gtgtccgtag tagttgag
//
LOCUS       DATAIR010000337         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_332718, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000337 DATAIR010000000
VERSION     DATAIR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332718"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VHM69_08280"
     CDS             <1..225
                     /locus_tag="VHM69_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740428.1"
                     /translation="VMAGALLAFTLSFDDFVITFFVSGVGSSTLPLKIYSMIKFGVSP
                     VINALSTVVLVATMFLIFGGSRIFVNREDS"
     gene            228..1640
                     /locus_tag="VHM69_08285"
     CDS             228..1640
                     /locus_tag="VHM69_08285"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2740429.1"
                     /translation="MPDIGRLNVINPATGESIEEIATSTREDLDLAAERAQHGFAEWR
                     SFAGLDRAEVFHEISRSLREAADELAEIMTREGGKPYIENHDEVTWTAACFDYYGEIA
                     RDNVGYLPAPIEPQQLALVIREPVGTVACIVPWNYPLLLAAWKVAPALAAGNAVILKP
                     SEETPLATRRMAELIEGLPEGLLQVVYGAGDVGEMLVRHPGVDMVAFTGSQETGRKVG
                     VTAAKRLIRANLELGGKDPFIICDDVDVEIAAQGAVWAACLNAGQVCTASERFYVERK
                     IADDFVEASREFVESLNVGDPMDRSTDVGPLVNAKGREKVERHVGEAIQSGATLVTGG
                     ERVGDRGFFYRPTVLVGVEPSMRVMREETFGPVVPIVRVDSLDEAIEQANSVPYGLGA
                     NVYTRDFEKMLRCMREIRAGTVWINDPLTDNDAAPFGGQKGSGIGRELGREGLEAFQE
                     SKHVHIDPKVEKKEWWYPYG"
     gene            1656..>2114
                     /locus_tag="VHM69_08290"
     CDS             1656..>2114
                     /locus_tag="VHM69_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2740430.1"
                     /translation="MSDRPEMISTSVYRVDAHSISNAINVLLLENDDDWTLVDTGVVS
                     SVGRIRDALSALGSGPEDLKRIFLSHQHNDHTGGLQGLLEWAPDAEVGAPEHEAEVIS
                     GRRPMDPASNRVIRYLARNTELPPAPVEKILREGDLVSGFRVVATPGHSRG"
BASE COUNT          416 a          623 c          722 g          353 t
ORIGIN      
        1 gtgatggccg gggcgcttct cgccttcacc ctctcattcg atgacttcgt cataacgttc
       61 ttcgtgtctg gcgtggggtc gagcacgctg ccgctcaaga tttactcgat gatcaagttc
      121 ggcgtgagcc ccgtcatcaa cgcgctctcg acggtggtgc tggtcgcgac gatgttcctg
      181 atcttcggcg gaagccgcat cttcgtcaac agggaggact cctgaccatg ccggacatcg
      241 gccgcctgaa cgtcatcaac ccggcaaccg gggagagcat tgaggagata gcgacttcga
      301 cccgcgaaga cctggacctg gccgcggaga gagcgcagca cggctttgcg gaatggcgca
      361 gcttcgctgg gctcgacagg gccgaggtgt tccacgagat ctcgcgctcc ttgcgcgaag
      421 cggcggacga gctggctgag atcatgacgc gcgagggcgg caagccgtat atcgagaacc
      481 acgacgaggt aacgtggacg gcggcctgct tcgactacta cggggagatc gcccgcgaca
      541 acgtcggcta cctccccgcc cccatcgaac cccagcagct cgcactggta ataagggaac
      601 ccgtcggcac cgtcgcatgc atcgtcccgt ggaactaccc gctcctgctc gctgcatgga
      661 aggtcgcacc ggccctcgcc gcggggaacg ccgtcatcct caaaccatcc gaggagacgc
      721 ctctcgcgac gcgcaggatg gccgagttga tcgagggcct ccctgaagga ctcctccagg
      781 tcgtctacgg tgccggcgat gtcggggaga tgctggtcag gcaccccggt gtggacatgg
      841 tggcgttcac agggagccag gagacgggca ggaaggtcgg cgtcacggct gcgaaaaggc
      901 tcatcagggc gaaccttgaa ctcggcggga aggacccatt cataatctgc gacgacgtgg
      961 acgtggagat agccgcgcag ggcgcggtgt gggccgcatg cctgaacgca gggcaggtct
     1021 gcaccgcctc ggagcgcttc tacgtggaga ggaagatagc ggacgacttc gtggaagcgt
     1081 cgagggagtt cgtcgagtct ctgaacgtcg gggaccccat ggaccgctcg acggacgtag
     1141 gtcctctggt caacgcgaag ggccgcgaga aggtcgagcg gcacgtcggt gaggcgattc
     1201 agagtggagc gacgctcgtc accggcggtg agcgggtcgg cgacaggggc ttcttctaca
     1261 ggccgaccgt gctcgtcggg gtcgagccgt cgatgagggt gatgcgcgag gagaccttcg
     1321 ggcccgtcgt ccccatagtc cgggtagaca gtcttgatga agccatagag caggccaact
     1381 ccgtgcctta cgggctcggg gccaacgtat acacgcggga cttcgagaag atgctcaggt
     1441 gcatgcgcga aatacgagct ggaacggtct ggatcaacga cccactcacg gacaacgacg
     1501 cggcgccctt cggcggccag aaagggagcg gcataggaag ggaactcggg cgcgagggac
     1561 tcgaagcctt ccaggagtca aagcacgtcc acatcgaccc gaaggtcgag aagaaggaat
     1621 ggtggtatcc ctatgggtag gtttcaggtg tccgcatgag tgaccggccg gagatgatct
     1681 ccaccagcgt ctaccgcgtg gacgcacact ctatttcgaa cgccatcaac gtgttgctac
     1741 tggagaacga cgacgactgg acgctcgtag acaccggcgt cgtgagcagc gtggggcgta
     1801 tacgggatgc cttgagtgcg ctcgggtcgg ggcctgaaga cctcaagcgg atctttctgt
     1861 cgcaccagca caacgaccac accggcggtc tgcaaggttt gctggaatgg gcgcctgacg
     1921 cggaggtagg agcgccggag cacgaggcgg aggtgatctc gggccggagg ccgatggatc
     1981 ccgcgagcaa cagggttatt cgttacctgg ccagaaacac cgagctaccg ccggctcctg
     2041 tcgagaagat cctgcgcgag ggcgatctcg tatcaggctt ccgtgtcgtc gccacgcccg
     2101 gacatagccg gggt
//
LOCUS       DATAIR010000338         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_148805, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000338 DATAIR010000000
VERSION     DATAIR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148805"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VHM69_08295"
     CDS             <1..168
                     /locus_tag="VHM69_08295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF020742.4"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2740431.1"
                     /translation="KRGFVVAPWDGTEETEQKIKDDTKATIRLLPFEREEGEDLVSGK
                     PGKTAIFARAY"
     gene            275..1219
                     /locus_tag="VHM69_08300"
     CDS             275..1219
                     /locus_tag="VHM69_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740432.1"
                     /translation="MGSLRTTRHIMRLRVALLLLTVVAGGGLGLAGAMAGVEAESYSA
                     YADPKKPVLSYLLSDERNVEEFQREFGLGDGRVQEVLAVIEKENSTLSREYDESEQIV
                     DSGAGEAEIEDRIDDSDFDEKLKQTIAQTKSEVEDLLPDGRTGDLESWVNEQWRAETA
                     QYSSESEPSYQVSSTGYSCGVWATYYKGHTRYEVALPNKKVKFDGGHRVRISIAGKGT
                     RTRAPVKEVGPWNITDNYWQARRYRDRWKNLPRCMPEAEAAFYDNYNRGEDQFGREVR
                     NPAGFDMTIRVARKLHVAHKIKREGKIRVRIYYPWVRR"
     regulatory      1373..1496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1528..2724
                     /locus_tag="VHM69_08305"
     CDS             1528..2724
                     /locus_tag="VHM69_08305"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX2740433.1"
                     /translation="MRVWHNAVERDREEAALLPSMTLDPGAGASHAPARKGRSHHCIG
                     SFEPELGGSLREVTLAYETWGELNEAADNAVLLVHALTGDSRAAGGPSAEYKRGGWWD
                     PMVGPGLAVDTEKYFVVCSNVIGGCSGSTGPASVEPGTGRHYGMRFPVVTIRDMVRAQ
                     RRLLDALGVRRLALVAGGSIGGQQALEWAVEFPDFVQKAVPIAATGALGPQGVGMSEI
                     GRRAIMADPDWQDGYYYGTGRSPDRGLAIARMAGMMTYQSAAGQWERFGRRPASRPAL
                     HEEFGGRFEIESYLHYQGRELTGRFDANSYLYLSRAMDIYDVANGYKSDEEAYSRIEG
                     EVLFVGITSDWLFPAAEVRDAAERASRAGAYAHYAEIDTLSGHDAFLKDWKELTEAIE
                     PFVTRS"
     gene            2771..4078
                     /locus_tag="VHM69_08310"
     CDS             2771..4078
                     /locus_tag="VHM69_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEX2740434.1"
                     /translation="MTDIADEKIQERDYGFDTLALHAGQEVDSNTTARAVPIYQSTSY
                     VFHDTDHAANLFSLAEEGNIYTRIMNPTTDVFEKRVAALEKGVGALATASGQAAQALA
                     ILNIMGAGDEIVSAAELYGGTYNQFHYTLPKVGIDVKFVDSTKPESFRDAITDRTKAI
                     YAETVGNPALNTLDIEAAAAVAHDAGVPLIIDNTLPSPYLVNPLEHGADIVVHSATKF
                     IGGHGTSIGGVIVDGGRFPWDNGKFPGFTEPDPSYHGLEIYPTLGELSFILKTRVQML
                     RDYGPALSPFNSFLFLQGLETLPLRMERHSTNAQAVAEFLQGHPKVNWVNYPGLEDHP
                     SHDLAAKYHREGLFGAILGFGIEGGLEAGKRFIDGLQLHSLLANVGDAKSLVIHPAST
                     THSQLSMDEQKSTGVTDDYVRLSVGLESIDDILYDLDQALNGA"
BASE COUNT          880 a         1185 c         1371 g          687 t
ORIGIN      
        1 aagcgtggct tcgtcgtcgc accctgggat ggtacggagg agacggagca gaagatcaaa
       61 gacgatacga aggccacgat ccggctcctg cccttcgaga gggaggaagg ggaggacctc
      121 gtctcaggca agccgggcaa gacggccatc ttcgcccggg cctattgaga aggaaggatc
      181 gaacggttcc ccgatgggaa gccaggggcg acttcgcatt ggcctgacgc tcgtgtaaaa
      241 tttgtttcgt tgagaagtaa gggaggggga ctccatggga tcgttgcgca cgacgcgcca
      301 cattatgcgc ttgcgggtgg cattgctgtt gttgaccgtt gttgctggtg gggggctcgg
      361 gctggccgga gcgatggctg gagtcgaggc ggagagctac tctgcctacg ccgacccgaa
      421 gaagcccgtc ctttcctatt tactatcgga tgagcggaac gtcgaggaat ttcaaaggga
      481 gttcggcctg ggcgacgggc gggttcaaga ggtccttgcc gtcatcgaga aggagaacag
      541 caccctctcc agggaatacg acgagagcga gcagatcgtg gactccggtg cgggggaggc
      601 cgagatagag gacaggatcg acgactccga cttcgacgag aagctgaagc agaccatcgc
      661 gcagacgaag tcggaagtag aggacctgtt gcctgacggt aggacgggcg atctagagtc
      721 gtgggtgaac gagcaatggc gagccgagac tgcacagtac agctccgaga gcgagccctc
      781 atatcaggtc tcgtccacgg gctactcctg cggggtctgg gccacatact acaaaggtca
      841 cacgaggtac gaggtcgccc tcccgaacaa gaaggtcaag ttcgacggcg gtcacagagt
      901 caggatctct attgccggca agggcacgag gacgcgggcg ccggtcaagg aagtgggacc
      961 gtggaatatc accgacaact actggcaggc ccgcaggtac agggacaggt ggaaaaacct
     1021 cccccgttgc atgcccgagg ccgaggcagc tttctacgac aattacaatc gtggcgagga
     1081 ccagttcggg agagaggtgc gcaatcccgc tggcttcgac atgaccatac gcgttgcccg
     1141 caagctacac gtcgcgcata agatcaagcg cgaagggaag atcagagttc gcatctacta
     1201 tccctgggtg cgccgctagc ggaaggggga ggagaactgg agcacatctc cttgatggag
     1261 agagggtctt ttcctatctc aaagtagcta gaagggctca ggaagtcgcc ttgaccgggg
     1321 tctctcttga cgttgagacc ccgggtgcgt tagcatccac gcctaacgcg aactcgcatc
     1381 gagagcggtg gagggaactg gccctgagaa accgcggcaa ccggcggcag gtggtacagg
     1441 ccgcaaggtg ccaagtccag caggaggcgg cgatagcctc tcctgggaga tgtggggaga
     1501 gccggatggc ctcttctacg gagcgggttg cgcgtatggc acaacgccgt agaacgcgac
     1561 cgagaggagg ctgcattact gccgagtatg acactagacc ctggggccgg cgctagtcac
     1621 gccccggccc gaaagggtag atcccatcac tgcataggct ctttcgagcc ggaactcggc
     1681 ggaagcctgc gagaggtcac cctggcttac gagacctggg gcgagttgaa cgaggccgcg
     1741 gacaacgccg tactcctcgt ccacgcccta acaggggact cgcgcgcagc aggaggccct
     1801 tccgcggagt acaagcgcgg cggctggtgg gacccgatgg tcggaccggg gttggccgtg
     1861 gacaccgaaa aatacttcgt cgtctgttcc aacgtcatcg gtggttgctc gggcagcacg
     1921 ggaccagcct ccgtcgaacc ggggacaggg cggcactacg ggatgcgctt ccccgttgtg
     1981 acgatcaggg acatggtcag ggcgcagagg cggttgctcg acgctcttgg cgtgcggcgg
     2041 ctcgccctcg tggcgggcgg ttccatcggg gggcagcagg cgctcgagtg ggccgtcgag
     2101 ttccccgact tcgtgcagaa ggccgttccg atcgccgcca cgggggctct cgggccgcag
     2161 ggagtcggga tgagcgagat cggacgccgc gccatcatgg ccgaccctga ctggcaggat
     2221 ggctactact acggcacggg aaggagcccg gacagaggac tcgctatcgc ccgcatggca
     2281 gggatgatga cctaccagag cgcagcggga cagtgggaga ggttcggacg caggccggcg
     2341 agccgtcccg cgctgcacga agaattcggc gggaggttcg agatcgagag ctacctccac
     2401 taccagggga gggagctcac gggtcgcttc gacgccaact cctacctcta cctatcgagg
     2461 gctatggaca tctacgacgt ggcgaacggc tacaagtcag acgaagaggc gtattcgcgc
     2521 atcgagggcg aggtgctgtt cgtagggatc acgagcgact ggctcttccc cgcggccgag
     2581 gtacgagatg cggcggagag ggcgagcagg gcgggagcgt acgcacacta cgccgagata
     2641 gatacactaa gcggccatga tgctttcctg aaggactgga aagaactgac agaagcgata
     2701 gaacctttcg taaccagaag ctgactgctg aaagctgata cctgatggct ctcgaccgaa
     2761 gggagagaaa atgacagaca tcgcagacga gaagatccag gagcgcgact acgggttcga
     2821 caccctggcg ctgcacgcag ggcaggaggt agactcgaac acgacggcgc gggccgtccc
     2881 gatctaccag tccacctcct acgtcttcca cgacacggac cacgcggcta acctcttctc
     2941 gctcgctgaa gagggcaaca tctacaccag gatcatgaac ccgacgaccg acgtgttcga
     3001 gaagcgggta gcggcgctcg agaagggtgt tggcgccctc gcgaccgcct ctggccaggc
     3061 ggcgcaggcg ctggctatcc tgaacatcat gggggcgggg gacgagatcg tctccgcagc
     3121 ggagctctac ggcggcacct acaaccagtt tcactacacg cttcccaagg tgggtataga
     3181 cgtcaagttc gtggactcga cgaagccgga gagcttcagg gacgcgatca cggacagaac
     3241 gaaagccatc tacgctgaga ccgttgggaa cccggcactc aacaccctgg acatcgaggc
     3301 cgccgcggcg gtggcgcacg acgccggcgt ccctctcatc atagacaaca cgctgccctc
     3361 gccatatctg gtgaacccgc ttgagcatgg ggccgacatc gtcgtgcact ctgcgaccaa
     3421 gttcatcggg gggcacggaa cttccatcgg cggcgtcata gtggacggcg gcaggttccc
     3481 atgggacaac gggaagttcc cggggttcac cgagcctgac ccttcctatc acgggctgga
     3541 gatctacccg actctgggtg agctttcgtt catcttgaag accagggtac agatgctcag
     3601 agattacggt ccggccttga gcccgttcaa cagcttcctg ttcctccagg gcctcgagac
     3661 gctgcccttg aggatggagc gccattcgac gaacgcgcag gccgtcgccg agttcctgca
     3721 gggccatccc aaggtcaact gggtcaatta tcccggtctc gaagaccatc cttcgcacga
     3781 cctggcggcg aagtaccaca gggaaggtct cttcggtgcg atcctcggct tcggcatcga
     3841 aggtggcctc gaggcaggca agcgctttat cgacgggctg cagttgcaca gcctcctcgc
     3901 gaacgttggg gatgcgaaga gcctggtcat acacccggct tcgacgaccc actcgcagct
     3961 ctcgatggac gagcagaagt caacgggcgt caccgacgac tacgtcaggc tctccgtcgg
     4021 cctcgagtcc atcgacgaca tcctgtacga cctggatcag gcactgaacg gagcgtgagc
     4081 tcattggaga aacctctcgt cataaagttc gggggtactt ctg
//
LOCUS       DATAIR010000339         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_390927, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000339 DATAIR010000000
VERSION     DATAIR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390927"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..669)
                     /locus_tag="VHM69_08315"
     CDS             complement(190..669)
                     /locus_tag="VHM69_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2740435.1"
                     /translation="MEEQIRSEILKTIPYGFYITGVVGPDGEANGFTTCWVSQVSFEP
                     QQVVVAARKDHHTHDLIEDGGVFSLNFLDSGQEELARKFTEPLKPENGAIAGSPYSQG
                     ETGAPLFDEAFAHLECRVVGKMEAGDHTVYLGEVAAANLVRPADILTDLDTPMEYGG"
     gene            823..1269
                     /locus_tag="VHM69_08320"
     CDS             823..1269
                     /locus_tag="VHM69_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740436.1"
                     /translation="MPDRMGNTALKLVVVGLVLVAGFVARVSYEQLVYPGAPAEAQTD
                     QYDCASFGSQESAQAELDRDPSDPNNLDPDGNGQACDDYPYGTSGTNSASPTSSTPSN
                     ASASPRPQKSENLFDSGGPTEGPVPLMPDGECPVEYPVKHNGLCYP"
     gene            complement(1792..>2787)
                     /locus_tag="VHM69_08325"
     CDS             complement(1792..>2787)
                     /locus_tag="VHM69_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740437.1"
                     /translation="FFAAQISRRLRELEDFLLVPDVYRDFSTGRCVTFEAPPDIASPE
                     DSHYEEVSESLVRLALTEGVFLADLSPGRFVVDGSTGEVWLSDPTEAFSMDPERLRGA
                     AEVLAAVRRGDVDGMVRAMPLAGSFVPRDGSDLRRELRETMGSLGGPLWREHSLEEVR
                     DRGLEAIRRGGARLHVEIAQMAEFLVGAEKIRSGGPRIVAAAEAAEILIARYRDPVYL
                     AARVARRVAQPDALADYPRQVHALLEELKDGEVEVRFRHAGLDELISKVDILANRLVV
                     ALLIAALIIGSSMIGIFLEGGIKILGVSLIGLIGFVFAAILGLLLLVGIIRSGRL"
BASE COUNT          531 a          861 c          889 g          506 t
ORIGIN      
        1 ccgcgaagga gaccacggcg tcgtagggat cattcgccca cgggctatgt gccctgacgg
       61 ccgggatctc ggcgctgaag tcgaacagca tcaagaagga cgcgaccgcg agcgccgcgg
      121 cattggcggc ccggcgagtc gcttcctatt gccggttgtt gcgcaagggg ccattcggtg
      181 acgcctctac tagcccccgt actccatagg cgtatcgagg tcggtgagga tgtctgcggg
      241 gcgcaccagg ttggcggcgg cgacctcgcc gaggtagacc gtgtgatccc ctgcctccat
      301 cttgcctact accctgcact ccaggtgggc gaacgcctcg tcgaagagcg gggctcccgt
      361 ctctccctgg gagtaaggcg agccggcgat ggcgccgttc tcgggcttga ggggctcggt
      421 gaacttgcgg gcgagttctt cctgcccgga gtcgaggaag ttcagggaga agacgccgcc
      481 atcttcgatc agatcgtgcg tgtgatggtc cttgcgggct gcgactacga cctgctgcgg
      541 ctcgaacgat acctgcgaga cccagcaggt ggtgaacccg ttggcctcac cgtcagggcc
      601 aacgacgccg gtgatgtaga aaccgtaggg gatggtcttc aggatctcgc tgcgaatctg
      661 ctcttccatg ctctcctgcc tctccgtgga aacggtcaga tggcattcta caggcgtttg
      721 gagcgaacga tgcgggtggc cgcgtttgga ggtgaaggtt gctttctctc accataatac
      781 tccccgttgc ggggtatccg tctacgtgca tgatggcgta caatgcccga taggatggga
      841 aacacagcgt tgaagctggt agtcgtgggg ctggtgctcg tggccgggtt tgttgcgcgc
      901 gtttcctacg aacagctagt ctacccgggt gcgccggccg aagcgcagac cgaccagtac
      961 gactgcgcca gcttcggttc tcaggagtcg gcgcaagccg agttggaccg cgatcccagc
     1021 gacccgaaca acctcgaccc ggacggcaac gggcaggcct gtgacgacta cccctacggc
     1081 acgagcggca ccaatagcgc ctcccctaca agcagtaccc cgagcaacgc gtctgcctcg
     1141 ccacgaccac agaagagcga aaatctgttc gactcaggcg gccctacgga aggaccggtg
     1201 cctttgatgc ctgacggcga atgtcccgtg gagtatccgg tgaaacacaa cggactctgc
     1261 tacccgtagt ccattcctgc cggaataaag cctcgaacag gctctgttgg aagcggaaac
     1321 cttcttcgcc ggtgatcctg ccgccgcaac catcacgggc aagtgggtga tgaagggggc
     1381 atgtgcggcc aattccctcc caacttcgac agaaagcacc attggatgca ggaagaggtt
     1441 cagctataca ccatcttcgg cataccatat ggcgagttga ggatccaatg cacaggttcg
     1501 gacctcatcc gctgcccggc agccggtgca gaatgcaaac ggggtgacgg ggcacgatct
     1561 ttcacgacgc gcaggtggat gggagagttg gacaagcaga gtgagatatt agatgggttg
     1621 gtgtaaccgt tacgtcgctt cgcctctggt gtcgcaggat tcgaatacat gtttgatcac
     1681 gcccggctcg ctcactatgt ccatgcctgc tctacaagca gtgggtcgta attggctgtc
     1741 atctcctcat cacgagtcca ggcagtaata cggaggcgtc ggtgtgctgg gttacaggcg
     1801 tcccgagcgg ataatgccga cgagcagcag gaggccaagt atcgcggcga agacgaagcc
     1861 gatcaggccg atgaggctga cgccgaggat cttgatgccg ccctcgagga agatcccaat
     1921 catcgaggaa ccgatgatga gcgcggctat gaggagggcg acgacgaggc ggttggcgag
     1981 gatatccact tttgaaatga gctcgtccag accggcgtgc ctgaaacgaa cctcgacctc
     2041 gccgtccttg agctcctcga ggagcgcatg gacctggcga gggtaatcag cgagggcgtc
     2101 gggctgggcg accctgcgcg ccacgcgggc cgctaggtag acgggatccc tgtatcgggc
     2161 gatgaggatc tcggccgcct cggccgccgc aacgatccgg ggtcctccgc ttctgatctt
     2221 ctcggccccg accaggaact cggccatttg cgctatctcg acgtgcagcc gcgcgccccc
     2281 tctacggatg gcctcgagac ccctgtccct gacctcctct agggagtgtt cgcgccacag
     2341 agggccgccc agcgagccca tggtctcgcg caactcgcgc ctgaggtccg agccgtcccg
     2401 gggtacgaaa ctccctgcca gcggcatcgc gcgaaccatt ccgtccacgt ccccccggcg
     2461 cacagcggcc agaacctccg ctgcgccccg cagcctctcc gggtccatgc tgaacgcctc
     2521 cgtcgggtcg gagagccaga cctctcctgt gctgccgtcg acgacgaagc gcccgggtga
     2581 caggtccgcg aggaagactc cctctgtcag cgcgagccgc accagcgatt cggagacctc
     2641 ctcgtaatga ctgtcttcgg gggacgcaat atccgggggc gcctcaaacg tgacgcagcg
     2701 gcccgtcgag aaatccctgt agacgtcagg gacgagcagg aaatcttcca gctctctcag
     2761 gcggcgggag atctgggcgg cgaagaa
//
LOCUS       DATAIR010000340         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_437220, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000340 DATAIR010000000
VERSION     DATAIR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437220"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VHM69_08330"
     CDS             <1..656
                     /locus_tag="VHM69_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEX2740438.1"
                     /translation="IRDLIAILPDPLRQRSLTHPSVADPYESNGRLEFLGDSVLNNRV
                     AELVFHGYPELLEGDLTRIRAHLVRKSFLAMVGRSEGIEEIIESSVINDSVIADTVEA
                     IIGAAYLIDRELALRTLDEIFNPAEVDTDELRDWKTLLQETLQADGLRPTYRVISKQG
                     PAHRPSFISRVSVDGREVATGAGSSIKESEQTAARNALEILGIRPVPRSPVEVHVGA"
     gene            869..>3034
                     /locus_tag="VHM69_08335"
     CDS             869..>3034
                     /locus_tag="VHM69_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2740439.1"
                     /translation="MLSAIYIKGFKTFARPVRMPLEAGITSIVGPNGSGKSNITDAVL
                     FALGEQSPGVLRAGAMSELIFSGSEMLPAANAAEVTLVFDNDAGRISLPYKEVSITRR
                     ISRAGETEYRINGSRSRLADVRAVAGEAGLGRHSILRQGAVDSIVAGGASACRLALEE
                     AAGLGVYRRRRVSASRRLERSDVQLERSRQLEAELEGQLRRIEREAAAAREYREIEVR
                     YRELSLAHLYGIATRELDGLRQKLDAANGHVAKLAASERDIREEERKVERRLHHLEGD
                     FRKTELRIESLEDSAERLRTEALRSDRILLRLEARRGREGERRLAVSRLEDELGRISQ
                     LTERLQERVSVLEAERAGSREELERKLKLLSLARESNADAEREKGRLSRELESLRTRI
                     AAFASNGHAGVISEEDIARLKAGISSFDEGVVKSPRLRELRDVVGDALARIDESNEQV
                     SRRRGALAAAVGRAESRVRALRETAPVGAGTRLYEVIRARNGYEVAVEAALGEYGAGV
                     LAENLDEGMKLLSDAEPVAVRLDARAMEANGTVRGKPLIECVEVLDARYDEAVQRLLT
                     GIFVVERPDDGANANGYVAVTRDGFRLTRTGVTLRSGSGNFAREARLTAWMEHLESLK
                     HGPGGLLYDVKENLSDASARLDSLAGTVQNLSAIAGRVSRAHALISRTATRRRAAAES
                     SRRQVLERERTTRDLSARASETEKSFAEAARTADHARDEL"
BASE COUNT          615 a          923 c         1015 g          481 t
ORIGIN      
        1 ctataagaga cctcatagcg atccttccgg accctttgag gcagcgctcg cttacccacc
       61 cgagcgtcgc cgacccgtac gaatcgaacg ggcggctgga gtttctgggc gactccgtgt
      121 tgaacaaccg cgtcgccgag ctggtatttc acggctatcc ggagcttttg gaaggggacc
      181 tcacgcgcat cagagcccac cttgtgagga agtccttcct tgcgatggtc ggccggagcg
      241 agggcatcga ggagatcatc gagagctccg tcataaacga ctcggtcatc gccgacaccg
      301 tcgaggccat tatcggggcg gcatatctta tagacaggga actggcgctc aggaccctgg
      361 atgagatctt caaccccgca gaggttgata ccgacgagct gcgcgactgg aagacgctcc
      421 tccaggagac actgcaggcc gatggtctga ggccgacgta cagggtaatc tccaaacagg
      481 gacccgcgca caggccaagc tttatctcca gggtctccgt ggatggaagg gaagtcgcca
      541 caggcgcggg cagctccata aaggagagcg aacagaccgc agcccgcaac gccctcgaga
      601 tactgggcat ccgcccggtc ccgcgttccc ccgtcgaagt gcacgtcggg gcgtagctga
      661 gctacgcagg cttcaggcca caggcatcag gtcattatcc tagctgagaa caaatagttt
      721 gcggcacttt ctagaccatc gattccgata acacggtacg acagcgtacc tatacgcttg
      781 tctaacgccg aagcctgtaa cctgaagcct catttgcggc ccttttctgc gtgcgttccc
      841 ggcggaagtt gcgtaaaatg ttccttcagt gctctcggcg atctatatca agggcttcaa
      901 gaccttcgcc cggccggtca ggatgccgct ggaagcgggg attacatcga tcgtcgggcc
      961 aaacgggtca gggaagagca acatcactga cgctgtgctc ttcgcgctgg gggagcagag
     1021 tccaggggta ctgcgagcag gggcgatgag cgagttgatc ttctccggct cagagatgct
     1081 ccccgctgcc aacgccgcag aggtcaccct ggtattcgac aacgacgccg gcaggatctc
     1141 tctcccttat aaagaggtgt ccatcacgcg ccgcatctcc cgcgccgggg agacggagta
     1201 caggatcaac ggctcccgct cccgcctggc cgacgttcgc gctgtggcag gggaagctgg
     1261 cctgggacgc cacagcatcc tgcgccaggg agccgtggat tcgatagtcg ccggcggggc
     1321 ctcggcgtgc cgcctcgctc tcgaagaagc cgccgggctc ggcgtctaca ggaggaggcg
     1381 cgtctcggcc agccgccgtc tcgagaggtc agacgtccag ctcgagcgca gccgccagct
     1441 ggaggcggag cttgaaggcc agctccgcag gatcgaaagg gaagcggcgg cagcccgcga
     1501 gtacagggag atagaagtcc ggtaccgcga gctctccctg gcccacctct acgggatcgc
     1561 aacgagggaa ctggacggcc tgcgacagaa gctcgacgcg gccaatgggc acgttgcgaa
     1621 gctggcagcg agcgagcgag acattcgcga ggaagaacga aaggtggagc gccgactaca
     1681 tcacctggaa ggggacttca ggaaaacgga actccggatc gagtccctcg aggacagcgc
     1741 cgagcgcctg cgcaccgagg cgttgcgttc cgacaggatc ctgcttcgtc tcgaagcacg
     1801 ccggggacgc gaaggggaga ggcggctcgc cgtctcccgc ctggaagacg agctgggcag
     1861 gatttcccag ctcacggaaa ggctgcagga gagagtcagc gtgctcgaag ccgagcgggc
     1921 aggcagtcgg gaagagctgg aaaggaaact gaagcttctc tctctggcgc gggagagcaa
     1981 cgcggacgcc gaaagggaga aggggaggct ttcgcgggag ttggagagcc ttcgcactcg
     2041 catagccgcg ttcgcgtcca acggacacgc cggcgtaatc tccgaggagg acatcgcgcg
     2101 gctgaaggct gggatctcga gcttcgatga aggcgtggtg aagtcgccga ggctgcgcga
     2161 gttgcgggat gtggtcgggg atgcgcttgc gcggattgat gagagtaacg aacaagtgag
     2221 ccgcaggcgc ggggcgcttg ccgcggcggt cggcagagcc gagtcgcggg tgcgcgcgct
     2281 aagggagacg gccccagtag gcgcaggcac gcggctctac gaggtgatcc gggcccgcaa
     2341 tggctacgag gtcgcggtcg aagcagccct tggagagtac ggggccgggg tcctggccga
     2401 gaacctggac gaggggatga agctcctctc ggacgccgaa cccgttgccg tccgactgga
     2461 tgcccgcgcc atggaagcca acgggaccgt acgggggaaa cccctgatcg aatgcgtcga
     2521 ggttctggac gcgcgctacg atgaagccgt tcagcgcctt ttaacgggca ttttcgtcgt
     2581 ggagcgccct gacgacggcg ccaacgcaaa cggatacgtt gccgttacca gggatggttt
     2641 ccgcctcacg cgtacaggcg tcaccctgag atccggttcc ggtaacttcg cccgtgaggc
     2701 gcgcctcaca gcctggatgg aacacctcga atcactgaag cacggccccg gaggccttct
     2761 ctacgatgtg aaggagaacc tctctgacgc ctccgcccga ctggactcgc tcgcaggaac
     2821 ggtgcagaac ctctcagcca ttgcgggacg tgtctcccgg gcacacgccc tgatctcacg
     2881 caccgccacg cgacgcaggg ccgccgcaga gagctctcgc aggcaagtcc tggagcgcga
     2941 gaggacgact cgcgacctct cggccagggc gtccgagacg gagaaatcct tcgccgaagc
     3001 cgcgaggacc gcggaccacg cgagagacga gctc
//
LOCUS       DATAIR010000341         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_437235, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000341 DATAIR010000000
VERSION     DATAIR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437235"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VHM69_08340"
     CDS             <1..426
                     /locus_tag="VHM69_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2740440.1"
                     /translation="YIRKDASDEREEIKVEGDPESTDAPLVVLVNEGSASSSEILAGA
                     LRDNDRAPVIGETTFGTGTVLSEFVLRDGSSILLGVAEWLTPDGDFIRDTGITPDVKV
                     SLEKGTEPLAPDALRGLSREEALEKDAQLRAAYEKLEGQ"
     gene            505..972
                     /locus_tag="VHM69_08345"
     CDS             505..972
                     /locus_tag="VHM69_08345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2740441.1"
                     /translation="MAQRVRESIEVGAPVEDAFRYWSNFENFPTFMSNVEEVRLTAQG
                     TSHWRVKGPLGKSVEFDAKTTELDPNRGIGWNTVGGDVMTSGEARFDEVSPGRTRIEV
                     TMNYADPPGWAVGEIVANVLSNPERNLRADLENFARIVESGELGGPRAQTPSH"
     gene            complement(1359..2453)
                     /locus_tag="VHM69_08350"
     CDS             complement(1359..2453)
                     /locus_tag="VHM69_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740442.1"
                     /translation="MQPQRDLRDIEEIQELFEAGQETGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEVVDAGFLAETMGEDEDTQLVKEVLEGDLKTRYTGDAVQMYLDEI
                     GKTPLLTKAQETYLARRIERGDRRAKAHLTKANLRLVVSIAKKYAGRGVSLLDLIQEG
                     NIGLMRAVEKFDHRKGFKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRV
                     QRGLGAQLNRDPTDEEVASIMEVDAEEIVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKARMREALGSLPERERMVLEYRFGLTGGQPKTLEEVGERFDV
                     TRERIRQIQLTALAKIKSSPHAAGLRDLLE"
     gene            2588..3340
                     /locus_tag="VHM69_08355"
     CDS             2588..3340
                     /locus_tag="VHM69_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740443.1"
                     /translation="MGLRNLYKVFRETRNAAREQATLAVIGDSPKAAELATLLGAQRG
                     VRGAEVILSVSGDKVTLSGKAVDDPGEISLPPAGGKAMIEELAPRIVRALDEDYLVAL
                     AKGYPPFRRAVCEEIVRHNARQNAVIGALPIPGADMPVMTANQARMVLNIAAAHDEDL
                     SMDRAKELLGVLAAGFGLRAISRQAVKLVPVGGWAAAAVIGYSGTVAMGRATILYFER
                     GGQHVGEKEMEQIRDRATQEAKAFVSRMRRRK"
     gene            3487..>4565
                     /locus_tag="VHM69_08360"
     CDS             3487..>4565
                     /locus_tag="VHM69_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX2740444.1"
                     /translation="MRIFLALTAVQAVIVGVLMAMAEIRKRRQGPQKGFPWEDQPEIE
                     LESGEDRLKIYAYGVLLYGAMLDEIEAAESEIFIGTFIWKGDEVGRRFVDALARKARE
                     GVRVYAVFDGLANVFVPPTFKRFPEEIHYMDFRPLSGPARVMNPRNVFRFHRHVMSVD
                     GRVAFLGGYNIGSLFAAGWRDTHVRVSGHAAREVDNAFVDFWNSHRTGELPEIVPNRE
                     RDWNPAVRVRRNDPYMRIFPIREMYLEAIDRANSHVYLTHAYFVPDRALKAHLIDARE
                     RGVDIQLLVPEQSNHVVADWLARRHFYDLLAAGVRIFLYKHIMIHSKTATVDGLWSTI
                     GSANIDRYSMLGNYELNIEVYSSSLA"
BASE COUNT          871 a         1359 c         1494 g          841 t
ORIGIN      
        1 tacatccgca aggacgcttc ggacgagcgc gaggagatca aagtcgaagg cgacccggaa
       61 tcgacggacg cgccgctcgt ggttcttgtg aatgaggggt cggcctcgag ctccgagata
      121 ctggctggag ccctgaggga caatgaccgc gcgcccgtga tcggggagac gaccttcggc
      181 accggcaccg tgctctccga gttcgtcctc agggacggct cctccatcct gctcggtgtc
      241 gctgagtggc tgacccctga cggcgacttt atccgcgaca cagggatcac accggacgtg
      301 aaagtgtccc tggaaaaggg aacggaaccg ctcgcaccgg acgcccttcg cggcctctcc
      361 agggaggagg cactggagaa ggacgcccag ctgcgcgcgg catacgaaaa gctcgagggt
      421 cagtagcaag gttcacatga aaacgggttt ggaccgggca tcctcacggg caagatgagg
      481 ttggtagctc atagaaagga tgcaatggca cagcgagttc gtgagagcat agaggtagga
      541 gccccggtcg aggacgcctt ccgatactgg tccaacttcg agaacttccc gaccttcatg
      601 tccaacgtcg aggaggtcag gttgaccgcc cagggcacca gccactggcg ggtcaagggt
      661 ccactgggca agagcgtcga gttcgacgcc aagacgacgg agttggatcc caaccgcggc
      721 attggttgga atacggtcgg cggcgacgtg atgacgagcg gcgaggcgcg cttcgatgag
      781 gtctctcccg gccgcacgcg catcgaagtg acgatgaact acgccgaccc ccccggctgg
      841 gccgtcggtg agatcgtcgc gaacgtgctc tccaaccccg agcggaacct ccgggcggac
      901 ctggagaact tcgccaggat cgtcgagagc ggtgagcttg gtgggccacg ggcccagacg
      961 ccaagccact gatacaaaga gcctacttgt aggaaagcat cgaggccgcc aggagatccg
     1021 gcggcctcaa cgcccgtgtg gggaggaata gctcttgtcc atcgccattc ccatactgat
     1081 ccgcgtcttc ggcgaagccg cgagtcgcaa cccgaaccga cccggcagcg agggagacga
     1141 gacctcggaa agcgattcca accggcgcta gggaagaggg taagtaggtg tcaggcatca
     1201 ggcttcaggt atcaggtggg cacgatgata gacgtgcctg acgggaagtc acgacccaac
     1261 tggggacgat cgctgttagg gaccgtatcg gagacttcgg ttcgaaaaca tccgatagta
     1321 ccgcggaaaa ctgaagcctg atgcctgtag cctgatgcct attccaaaag atcgcgcagc
     1381 cctgcggcgt gggggctgct ctttatcttg gcgagggccg tgagctgtat ctggcggata
     1441 cgctcgcggg tgacgtcgaa gcgctctccg acctcttcga gggtcttggg ctggccgccc
     1501 gtgaggccga agcggtactc gaggaccatc cgctcgcgtt ctgggaggct gccgagggcc
     1561 tcgcgcatac gggccttcaa gagggatgac ttggcgaggt cgtgggggga ttcagactcc
     1621 tcgtccgcga taaagtcccc tagctctgag gagtggtcct ctccgacggg ggtctcgagg
     1681 gagatagaac gcctggagac gcgacggatg cgtactatct cctcggcatc gacctccatg
     1741 atggaggcga cctcctcatc cgtggggtca cggttgagct gggcgcccaa gccccgctgg
     1801 accctgtagt acttgttgat cttctcgacc atgtggacgg gtacccgtat cgtacgtccc
     1861 ttgtctgcta ttgccctggt gacggcctga cggatccacc acgtggcata cgtcgagaac
     1921 ttgaagccct tgcgatggtc gaacttctcg acggctcgca tgaggccgat gttgccctcc
     1981 tggatgaggt ccaaaagcga tacgcctctg ccagcgtact tcttggcgat ggagacgacg
     2041 agcctgaggt tggctttagt caagtgggcc ttcgcgcgcc ggtcgccgcg ctctatgcgc
     2101 cgcgcgaggt acgtttcctg cgctttggtg aggagggggg tcttgccgat ctcgtccagg
     2161 tacatctgga cggcgtcccc cgtatagcgg gtcttcaggt cgccttcgag aacctctttg
     2221 acgagttggg tgtcctcgtc ttcgcccatc gtctcggcga ggaaaccggc gtcgaccacc
     2281 tcgacaccgt gttcgcgcag gagcccgtag acctgttgaa tctcgtcggt cgagaggtcg
     2341 acctcctgca ggaggtcgag tacttcgctc gactccagcg tgccggtctc ctgaccggcc
     2401 tcgaagagct cctgtatttc ctcgatgtcc cgcaggtcac gttgaggctg caaaatttac
     2461 tccgggaact tcaggttgaa accctcctgt agccttcacg taatatatct taaaagggac
     2521 aataatcaat ttgattgcgc ggctcaggcg ggtcgtgtgg cctcaaccac gaggaggaag
     2581 agctgtgatg ggactgagaa atctgtacaa ggtctttcgc gagacccgca acgccgcgag
     2641 ggaacaggcg accctcgccg tcatcgggga ctcgccgaag gccgccgagc ttgccaccct
     2701 gctcggggcg cagaggggcg tgcgcggggc agaggtgatc ctctcggtct ccggagataa
     2761 ggtgacgctc tccggaaagg ccgtcgatga ccctggtgag atctctcttc ctccggcggg
     2821 cgggaaggcc atgatcgaag agctggcgcc ccgcatcgtg cgggcgctgg acgaggacta
     2881 tctggtcgcg ctagcgaagg ggtatccgcc gttcaggcgg gctgtgtgtg aggagatcgt
     2941 acgccacaac gcacgccaga acgccgtgat cggggctctc cccatccccg gcgccgacat
     3001 gcctgtgatg accgccaacc aggcgcggat ggtgctaaac atcgcggcgg cccacgatga
     3061 ggatctgtcc atggacaggg cgaaggagct cctcggggtc ctcgccgccg gtttcggcct
     3121 cagggccatc agccgtcagg ccgtgaagct cgtgcctgtg ggagggtggg ccgccgccgc
     3181 cgtcatcggc tactccggaa cggtggcgat gggccgggcc accatcctct acttcgagcg
     3241 cggcgggcaa cacgtgggcg agaaggagat ggagcagatc agggaccgcg ccacacagga
     3301 ggcgaaggca ttcgtctcaa gaatgcgccg caggaagtag tgaggtttca ggtcttggat
     3361 ttagctgaca ggtttggaat atccggcctg tgagctgagt gctctataat cgatagtatg
     3421 ttctctcctt ttcgacgggt cctatcggag gtacgctccg gcctctcgac ggcgcggtgg
     3481 cttggcatga ggatcttcct cgcgttgacg gctgtgcagg ctgtgatcgt aggcgttctc
     3541 atggcgatgg ccgagatccg caagcgtcgc caggggccgc agaaagggtt cccgtgggag
     3601 gatcagccgg agatagagct cgagtcgggc gaggacaggc tgaagatcta cgcctacggg
     3661 gtgctcctgt acggggccat gctcgatgag atagaggccg cggagagcga gatcttcatc
     3721 gggacgttta tctggaaggg ggacgaggtc ggtcgccggt tcgtggacgc cctggcgcgc
     3781 aaggctcgcg aaggcgtcag ggtctacgct gtcttcgacg ggctcgccaa cgtgttcgtg
     3841 ccccctactt tcaagaggtt ccccgaggag atccactaca tggacttccg tcctctgtct
     3901 ggacctgcga gggtaatgaa cccgaggaac gtcttccggt tccaccgcca cgtgatgagc
     3961 gttgacggca gggtcgcttt tctcggcggc tacaacatag ggtctctctt cgccgccggc
     4021 tggcgcgaca cgcacgtcag ggttagcggg cacgcggcca gggaggtgga caacgccttc
     4081 gtcgatttct ggaactccca ccgcaccggc gagcttccag agatagtccc gaacagggag
     4141 cgcgactgga accccgccgt cagggtccgg cgcaacgacc cttacatgag gatcttcccc
     4201 atcagggaga tgtacctgga ggcgatagac cgcgccaact cgcacgtcta cctgacccac
     4261 gcctacttcg tcccggacag ggccctgaaa gcgcacctga tcgacgcgcg ggagcgcggc
     4321 gtcgacatac agctactcgt ccccgagcag tcgaaccacg tcgtcgcgga ctggctcgcg
     4381 cggcgtcact tctacgacct cctcgcggca ggcgtcagga tcttcctcta caagcacatc
     4441 atgatccact ccaagacggc caccgtcgac ggcctgtggt caacgatcgg cagcgcgaac
     4501 atcgaccgct acagcatgct cggtaactac gagctgaaca tcgaggtcta cagcagcagc
     4561 ctcgc
//
LOCUS       DATAIR010000342         5958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_299419, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000342 DATAIR010000000
VERSION     DATAIR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299419"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VHM69_08365"
     CDS             complement(<1..1263)
                     /locus_tag="VHM69_08365"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX2740445.1"
                     /translation="MRSGNVILEAIETKKFGGELSDELVRAVVEGYEAGEVPDYQMSS
                     LLMAVFIQGMSYRETLALTRAMAESGKTYSFPECVDKHSTGGVGDKVSLTALPIAAAC
                     GAPVAKLSGRGLGTTGGTIDKLESIPGFSCHLSEERFRHQVEEVGLAIIEAGDLAPAD
                     KEIYALRDTTGTVDSLPLIGSSIVSKKVATGAGHLLYDVKRGSGAFMKTTAEAHELAE
                     LLVRLSEDLGISSSALITDMNNPLGSAVGNALEVRESVRLLKNEPVPDDLADEVSHVA
                     TRLLELKGIHDASAAVENALSSGAAYEKFREFVTAQGGDAEALDDLAVSTEVLEVGAP
                     RSGYVARIGASGIGSAALALGAGRQQKGDSIDPGSGIEILIKPGDHIEEGQPIAHLYG
                     EREAERAGRLVLEALEISDEPIEPPPAIL"
     gene            complement(1317..2285)
                     /locus_tag="VHM69_08370"
     CDS             complement(1317..2285)
                     /locus_tag="VHM69_08370"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX2740446.1"
                     /translation="MKIAVLTSGGVAPGMNAAVRAAAQAAFSRGWEVVAVEEGYYGLL
                     EGKMRPVDRGELWGYVQQGGTVLGTGRSSEFEEEGKQRAIELLREEGAEGLVVIGGGG
                     SISGGLALYELGFPTVGVPATIDNDIPGTELSIGVDTAVNTAASIIDRIKDATTSHRR
                     AMMVEVLGRDSGFLAVMSAIAGGADAVMVPEFETKPDELLELLNESYEKGKPRFTVVV
                     AEGASPSADELRDYVNDAGGSYQADLTVLGHIQSGGSPTSFDRILSSRMGAAAVEALT
                     DEDAGTMVGLDGDRIRRIPLDEVAGEQRPLDPELYTLAGVLSALPE"
     gene            2413..3240
                     /locus_tag="VHM69_08375"
     CDS             2413..3240
                     /locus_tag="VHM69_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2740447.1"
                     /translation="MVCCVRVMSFNVRGASHMGDGVNVWEKRAAINVETIRRYEPDLI
                     GFQEFHHGNREVYERELPGYTRLPGPVYGTAQVEEHAAIFFDPERFEDLDSGGFWLSD
                     TPDEYSTSWGNEVIRSANWAVLRCRQKGATFLHVNTHLDHVSEPARVEGSGLILRQSE
                     ETRTNHGELPTVVTGDFNCTPGTRPYRVFMEDGFVDTFLVAGNEDDAYTFHAFKGARF
                     TLADTDKPTGRIDWILVRDEAGRVKITSHEILRDGDEAAGTYPSDHYPVLAELELTG"
     gene            complement(3256..4422)
                     /locus_tag="VHM69_08380"
     CDS             complement(3256..4422)
                     /locus_tag="VHM69_08380"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX2740448.1"
                     /translation="MGRRAVVIVLDGLGAGNAPDAADFGDEGANTLANTAKAVGGIEA
                     PNLEALGLANVEEIEGIAASESPAASHGLMVELSAAKATLAGHWEMMGLVLEDPLPTY
                     PEGFPDEVLLRFESETGRGVIGNRPASGTQIIEEPGPEQEKTGAWILYTSADSVFQIA
                     AHTGVIPLDELYEASKKAHKMLIGEDRIMVERVIARPFHGKPGSYERENENRHDYGIT
                     PPSVTYLDRIEKAGHETVAVGKIRDIFDGEGITQHLPAPPDDSAKVDAILEALSRVES
                     GLIFANLVDFDAKYGHRRDPEGMAENLKRFDARLPELLDALTGEDLMIITADHGNDPT
                     FEGTDHTRERVPLLAVGGEPRDLGVRESFSDVGASVASWLGVEKAGLPGNNFLR"
     gene            4521..5003
                     /locus_tag="VHM69_08385"
     CDS             4521..5003
                     /locus_tag="VHM69_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740449.1"
                     /translation="MGSKNEVREAKAGDAAEIHSLACELAETVGDTPPTEDALRERLT
                     DLLDEPRARVLVAENEAGIVGAVSLWIKPDLAHGDVVVEVPMLAVAEDHRRTGVGRLL
                     MEEVRDIASDNGAYLIELVATSANVAAREFYNSLGFVEADVVPLEFVGSQEDPPDPDE
                     "
     gene            complement(5010..5549)
                     /locus_tag="VHM69_08390"
     CDS             complement(5010..5549)
                     /locus_tag="VHM69_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740450.1"
                     /translation="MAKRGYTRDTIRGGASEWDVSRPFSSYARTLAALLVHPVRFFEL
                     LPRIPDMRAPSLFLVFCGLPAAAVWLPFWGFYPALVALVLPLPLSFLLAGLYHVGVLG
                     GRYGYVVTWRVVVYPLGFFLPIAAVPVLGVVGAAYFGLVLVPIGLAKVHEVGWPRSVL
                     VCAAVGVLFGMVYYFLIIA"
     gene            5613..>5958
                     /locus_tag="VHM69_08395"
     CDS             5613..>5958
                     /locus_tag="VHM69_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2740451.1"
                     /translation="MNPSSSDRIKPLRRRGAALLGGALAIFVGVLYIVTLAPTVLPYG
                     TPDTLDSPMLQAEVWVLGIGHPTGYPTYMMLTHLFTYLPIGDPAYRVSLASAVYGTVA
                     VLIVYLVSLRLGG"
BASE COUNT         1036 a         1903 c         1912 g         1107 t
ORIGIN      
        1 caggatagca gggggaggct ctatgggctc gtcggagatt tctagcgcct cgagaacgag
       61 gcggccggct cgctccgctt cgcgttcgcc gtagaggtgg gctatgggtt ggccctcttc
      121 gatgtggtct ccaggcttta tcaggatctc gatgccggag cctgggtcga tgctgtcccc
      181 tttttgctgg cggcctgctc cgagggccag ggccgcgctg ccgatgccgg atgcaccgat
      241 gcgcgcgacg tatccgctgc gcggggcccc tacttcgagt acctcggtag agacggccag
      301 gtcatcgagg gcttctgcgt cgccaccctg ggcggtgacg aactccctga acttctcgta
      361 ggccgcgccc gaggagaggg cgttttcgac ggcggcggag gcatcgtgga tgcctttgag
      421 ctcgagaaga cgggtggcga cgtggctgac ctcgtcggcg aggtcgtctg ggacgggctc
      481 gtttttcagc agacgcacgc tctcacggac ttcgagggcg ttgccgaccg cgctcccaag
      541 agggttgttc atgtcagtga tcagggccga ggaacttatg ccgaggtctt cgctcagacg
      601 tacgaggagc tcagcgagtt cgtgggcctc agccgttgtc ttcatgaacg cccccgagcc
      661 gcgcttcacg tcgtagagca gatgtcccgc gcctgtggcg accttcttgg agacgataga
      721 ggagccgatg agcggcaggg agtctacggt gccagtcgta tcgcggaggg cgtagatctc
      781 cttgtccgcg ggtgccagat cccccgcctc gattatggct agcccgactt cttcgacctg
      841 atgacggaac cgttcttcgg agaggtggca cgagaagcct gggatggact ccagcttgtc
      901 tatggttcct ccagtggtgc ccaggcccct tcctgatagc ttggccaccg gtgctccgca
      961 ggctgcagct atcgggagcg cagtgaggct caccttgtcc ccgacgccgc ctgtggagtg
     1021 cttatcaacg cactctggga aggagtaggt tttgcccgat tcggccatcg cccgcgtcag
     1081 cgcgagcgtc tccctataac tcatcccctg gatgaagacg gccatgagga gcgaggacat
     1141 ctggtagtct ggcacctctc ccgcctcgta gccctccacc acggccctga cgagctcgtc
     1201 ggagagttcg ccgccgaatt tcttggtctc tatggcttcg agtatcacgt ttccggagcg
     1261 cattcggtcc ctgctttcgg tcgcggccat tctagcgggt gtgcgaatcc caggctctat
     1321 tcagggagcg cggataggac cccggcaagg gtgtagagct cggggtcgag agggcgttgc
     1381 tcgccagcga cctcatcgag ggggatccgg cggatcctgt ccccgtccag accgaccatc
     1441 gtccccgcat cctcgtcggt gagagcctcg accgctgccg cccccatgcg ggaggagagt
     1501 atccggtcga acgatgtagg actgccgcct gactggatgt gcccgaggac cgtgaggtcg
     1561 gcctggtatg agccgccggc gtcgttgacg taatcgcgga gctcatcggc tgatggagag
     1621 gcaccctcgg ccacgacgac ggtgaagcgc ggcttgccct tctcgtatga ttcgttgagg
     1681 agttccagca gttcgtcggg tttcgtctcg aactcgggga ccatgacagc gtcggcccct
     1741 cctgcgatgg cgctcatcac ggcgaggaag cccgagtcgc gccccagcac ctcgaccatc
     1801 atcgccctgc gatgggatgt cgtcgcgtcc tttatgcggt ctatgatcga tgcagccgtg
     1861 tttacggctg tgtccacccc gatggatagc tccgtgcccg ggatgtcgtt atctatggtg
     1921 gcagggacgc ccacagtagg aaagccgagc tcgtacagag ccagcccccc ggagatgctc
     1981 ccgccgcccc cgatcacgac gagcccctcg gcgccttcct cgcgcaacaa ctctatcgcc
     2041 ctctgtttgc cttcctcctc gaactcggag gagcggcccg tgccgagtac ggtccctccc
     2101 tgttgcacgt agccccagag ctcgccgcgg tcaacggggc gcatcttccc ttccagcagc
     2161 ccgtagtatc cttcttcgac cgcgacgacc tcccaacccc gcgagaaggc cgcctgcgcc
     2221 gcagccctca ccgcggcgtt catgccgggg gcgacgcccc cgctcgtcag cacggctatc
     2281 ttcaatgacc tctcctctcg ctctgactcc gagtaatgta cccgccggcg ccgcttttct
     2341 ccctggaccg agcataccgg ttctcattcc gcaggtagaa tccgttcggg ttggcgagga
     2401 ggagggcgag tcgtggtctg ttgcgtgcgc gtgatgagct tcaacgtcag gggcgcttct
     2461 cacatgggag atggcgtcaa cgtctgggag aagcgggcgg ccatcaacgt cgagaccatc
     2521 aggcgttacg aaccggacct gatcggcttc caggagttcc atcacgggaa ccgcgaagtc
     2581 tatgaaaggg aactgcctgg atacacccgc ctgccaggac ccgtatacgg tacggcgcag
     2641 gtcgaggagc acgccgccat cttcttcgac ccggagcggt tcgaggatct cgactccggc
     2701 gggttctggc tctccgacac gcccgatgag tattcgacga gctggggtaa cgaggtcatc
     2761 cgctcggcca actgggccgt cctgcggtgc aggcagaagg gcgctacctt cctgcacgtc
     2821 aacacgcacc tcgaccacgt cagcgagccc gcccgcgtcg aagggagcgg cctcatcctc
     2881 agacagtccg aggagacgcg caccaaccac ggagaactac cgaccgtcgt cacgggcgac
     2941 ttcaactgca cgcccggcac ccggccatac cgcgtcttca tggaagacgg cttcgtcgac
     3001 acctttctcg tcgccgggaa cgaggacgac gcatacacct tccacgcttt caaaggagcg
     3061 cggttcacgc tggccgacac ggacaagccc acgggacgca tcgattggat cctcgtccgc
     3121 gacgaagcgg gacgcgtaaa gataacctcg cacgagatcc tgcgcgacgg tgacgaggcg
     3181 gcagggacct accccagcga ccactatccc gtgcttgcgg agttggaact aactggctag
     3241 gaacgagcct cgcgctcacc gcaagaaatt gtttcccggg agaccagcct tctccacgcc
     3301 gagccacgac gcgaccgatg ctccaacgtc tgagaagctc tcgcgcacgc cgaggtcgcg
     3361 tggctcaccg cctacggcga ggagagggac cctctcgcgg gtgtgatccg ttccctcgaa
     3421 agtcgggtcg ttgccgtggt cggcggtgat aatcatcagg tcttcccctg tcagcgcatc
     3481 gagaagttcc ggcaggcggg cgtcgaagcg ctttaagttt tcggccatgc cttcagggtc
     3541 gcggcggtgg ccgtactttg cgtcgaagtc gacgaggttc gcgaagatca ggcccgattc
     3601 gacacgactc agagcctcga ggatcgcgtc tacctttgcc gaatcgtcgg gtggggcagg
     3661 gaggtgttgg gtgatgccct cgccgtcgaa gatgtccctg atcttgccta cagccacggt
     3721 ctcgtggccg gctttctcta tcctatccag ataggtcact gagggcggcg ttatgccgta
     3781 gtcgtggcgg ttctcgttct cgcgctcgta agagcctggc ttgccgtgga aggggcgggc
     3841 gatgacccgt tcgaccatta tcctgtcctc gccgatgagc atcttgtggg ccttcttcga
     3901 ggcttcgtag agttcatcga gtggaatcac gccggtgtgt gcggcgatct ggaagactga
     3961 gtctgcggag gtgtagagga tccaggctcc ggtcttctcc tgctcgggac cgggttcctc
     4021 tatgatctgt gtccccgagg cgggcctgtt gcctatgacc ccgcgccccg tctcgctctc
     4081 gaagcggagc aatacttcgt cggggaagcc ttcggggtag gtagggagcg gatcttcgag
     4141 cacgagaccc atcatctccc agtgtcctgc gagggtggcc ttggccgcgg ataactcgac
     4201 catcaggccg tgggaagccg caggagactc ggatgctgct atgccttcta tctcctcgac
     4261 gttggcgagc ccgagggctt cgaggttcgg cgcttcgatg ccgccgacgg cctttgctgt
     4321 gttcgccagg gtgttggcgc cttcgtcccc gaagtccgcc gcgtcggggg cgttgccggc
     4381 gcccagcccg tcaagcacga tcacgacagc cctgcgtccc atccgcctcc tttccgtgtc
     4441 agaattatct tagcgcctaa atccgttcgt cgccgcgctt cgtagggtaa gtagacacca
     4501 gactacggga ggaggtgttg atgggttcga aaaacgaggt aagggaggcg aaggccgggg
     4561 acgcagcgga gatccacagt ctcgcctgcg aactggccga gaccgttggc gatacgccgc
     4621 ccacggaaga cgccctcagg gagcggctta cggaccttct ggacgaaccc agggcgcggg
     4681 tcctcgtggc cgagaacgag gccgggatcg tcggagcggt gagcctctgg atcaaacccg
     4741 acctcgccca cggcgacgtc gtcgttgagg tgccgatgct cgccgtcgcg gaggaccaca
     4801 ggcggaccgg agtgggcagg ctcctgatgg aagaggtgcg cgacatcgcc tcagacaacg
     4861 gcgcgtacct gatcgaactg gtagccacca gcgctaacgt cgcggcacgc gagttctata
     4921 actctctcgg cttcgtcgaa gccgacgtgg tgcccctgga gttcgtcggt tctcaggaag
     4981 acccacccga ccccgacgaa tgaccccggt caggcgatga tcaggaaata gtagaccatc
     5041 ccgaacagca caccgacggc tgcgcagacg agcacggacc ggggccatcc gacctcatgc
     5101 accttcgcga gccctatcgg cacgagcacg agcccgaagt aagccgctcc cacgacccct
     5161 agcaccggaa cggcggcgat gggcaggaag aagccgagcg ggtacacgac gaccctccag
     5221 gtcacgacgt aaccgtacct gccaccgagc acgccgacgt ggtagagacc ggcgaggagg
     5281 aacgagagcg gcagcggcag gacgagggcg acgagcgcgg gatagaaccc ccagaaaggc
     5341 agccacacag cggcggcagg aagaccgcag aatacgagga agaggctcgg agccctcata
     5401 tcggggatcc tgggcagcag ttcgaagaac cggaccggat gtacgaggag cgcggcgagt
     5461 gtccgcgcgt agctcgaaaa cggccgcgag acgtcccact cgctggcgcc gccccggatc
     5521 gtgtcccgcg tgtagccgcg cttcgccacg cgcggagtat acagaaggcg ctcggggaga
     5581 gcgggacacg tacgtagtat tctgcccctg cgatgaatcc ttcttcgagc gacaggatca
     5641 aacctctcag gcggcggggc gcggcgttgc ttggtggagc actggcgatc ttcgtgggcg
     5701 tcctctacat cgtaacgctt gcgccgacgg tcctgccata cggcacccct gacacgctcg
     5761 attccccgat gctccaggcc gaggtctggg tactcgggat cggccatccg accggctacc
     5821 cgacgtacat gatgctgacg cacctattta cctacctgcc gataggtgac ccggcctacc
     5881 gcgttagcct cgcctccgcc gtctacggca ccgtggctgt gctcatcgtg tacctggtga
     5941 gcttgcggct tgggggac
//
LOCUS       DATAIR010000343         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_437290, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000343 DATAIR010000000
VERSION     DATAIR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437290"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VHM69_08400"
     CDS             complement(<1..752)
                     /locus_tag="VHM69_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2740452.1"
                     /translation="MALVGISSVTSLIIGLVLYSFAQDRLVTAENTLLKQRSQTANAG
                     AQDFLEGLRDPENMTLPPSKTYAEELVRSVADPTGLEVLYVGQEGKPLAARDGLGNPM
                     NARKAYEGHALDEGELEEIAASQRGAGRLVWCEERSGFVAVWPLTASDGTVKGVMIYD
                     VPRDELGETLAYLRYGILGAILTSILLAGAASLLLTRQVTRPLSETRDAAIRVASGDY
                     AATVPVKSRDELGEMARAFNYMAEEIEHYVGE"
     gene            complement(823..1512)
                     /locus_tag="VHM69_08405"
     CDS             complement(823..1512)
                     /locus_tag="VHM69_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2740453.1"
                     /translation="MDETILLVDDDAALLEVTSIVLASEGYRVLTAEDGVEALRIVGR
                     EGLDLVVLDIMLPRMSGFEVLKKMREHSDVPVVLLTAKSQSVDKVVGLELGADDYITK
                     PFDTKELLARIKAILRRFGRDGRGREGLLRLGPLELDPNGYTVSRSGETLDLTPTEFK
                     ILALLMRRPGQAFTRAQISEAVSTGSHYLASRYIDVHISRLRAKVERDPSKPEIIQTV
                     PSVGYRAARPA"
BASE COUNT          313 a          546 c          460 g          331 t
ORIGIN      
        1 atctccccga cgtagtgctc tatttcctcg gccatgtagt tgaaggccct ggccatctct
       61 ccgagctcgt ccctgctctt gacaggcacg gtcgccgcgt agtccccgga ggcgaccctt
      121 atggccgcgt cacgagtctc ggagagagga cgcgtgacct ggcgcgtgag cagcaggctc
      181 gccgcaccgg caaggaggat gctggtaagg atggccccga ggatcccgta gcgcaggtaa
      241 gcgagcgtct caccgagctc gtcgcgggga acgtcgtaga tcatgacccc cttcacggtc
      301 ccgtcggagg ccgtgagcgg ccacacggcg acgaaacccg agcgttcctc gcaccacacg
      361 agcctgcccg cgccccgctg cgatgcggcg atttcttcga gctcgccttc gtcgagtgcg
      421 tgcccctcgt atgctttgcg cgcattcatg ggattgccga gaccgtcgcg cgcggccaga
      481 ggtttacctt cttgccccac gtagagcacc tcgagacccg tcgggtcggc gaccgaccgt
      541 acgagctcct cagcgtaggt cttcgatgga gggagggtca tgttttccgg gtcgcgcagg
      601 ccttcgagga agtcctgcgc tcccgcgttc gccgtctgag aacgttgttt gagcagcgtg
      661 ttctccgcag tgaccagcct gtcctgggcg aaggaataga ggaccagacc aatgatcagg
      721 ctcgtcacgg aagagatgcc aacgagcgcc acgaagatcc tggccttgaa accgagcgtc
      781 gacagaagcc ccctgttatg cctcttgtct atgcctgtcc tcctaggctg gcctggccgc
      841 cctgtatccg acgctgggga cggtctgtat gatctccggc ttcgaagggt cgcgctcgac
      901 cttggctctt agacggctga tgtggacgtc tatgtagcgg ctcgccagat aatgcgaccc
      961 cgttgagacc gcctcggaga tctgggcccg cgtgaaggcc tgaccgggac ggcgcatgag
     1021 gagcgccagt atcttgaact cggtcggcgt caggtccagc gtctccccag acctgctgac
     1081 cgtgtagccg ttgggatcca actccagcgg acctagcctc aacagccctt ctctgcccct
     1141 gccgtcccga ccgaaacgcc tgaggatggc tttgatcctc gcgagcaact ccttggtgtc
     1201 gaagggcttg gtgatgtagt cgtcagcccc gagctcgagc ccgacgacct tgtctacgga
     1261 ctggctcttg gccgtgagca agacaacagg tacgtcactg tgctcccgca tcttcttcaa
     1321 gacctcgaac ccgctcatcc tcggcaacat gatgtcgagt accacaagat ccaaaccctc
     1381 gcgcccaact atccttagcg cctcaacccc atcttccgcc gttaataccc tgtaaccctc
     1441 agatgccagc acgatggacg tgacctctaa tagagccgcg tcgtcatcga ctagcaagat
     1501 agtttcgtcc aatctgactc cctaaaactt ccacacttac ccccgacatc cacaattccg
     1561 gggaccatcg tatcctaccc tatacacgcc cgccttcgta gcgataaccg aaaaggagaa
     1621 acttgcaagc gagacactca cattttatac
//
LOCUS       DATAIR010000344         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_149021, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000344 DATAIR010000000
VERSION     DATAIR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149021"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1256)
                     /locus_tag="VHM69_08410"
     CDS             complement(171..1256)
                     /locus_tag="VHM69_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2740454.1"
                     /translation="MVEGVVLGVDTHLDFHGAMVLDHLGRRLGESKVPTTTGGYRSLL
                     CWAEGFGPVRCAGVEGTGSYGAGLARYLKSTGITVMEVERPKRRHLKRNGKSDPKDAE
                     AAARAVLAGEAAGEPKSADGQVEMIRVLRAARCSAVKARSQAANQLQALRVTAPERLQ
                     RRLCGLSTSELVATCARFRPGNDPDGVETATRFALRSMARRHEVLSEEIAELDAQLDR
                     LVAEAAPGLISLPAIGTHHAATLLVVAGDNPERLGSEASFASLCGVSPVDASSGKVVR
                     HRLNRGGNRDANRALHMICVVRMGSDRRTRSYVARRTAEGKSKREIMRCLKRYIAREV
                     YRVLLSSTTVEPSPIRSERGFVGADVA"
     gene            1764..2192
                     /locus_tag="VHM69_08415"
     CDS             1764..2192
                     /locus_tag="VHM69_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740455.1"
                     /translation="MSQQTTNRTFDELARGLASGSITRGKALKLMGAALLGGTLASLG
                     IREAAADEACKPLNKKCRKDEQCCSGLCEGGTCAPACQVDSDCPDPGTQCQQAACNNG
                     VCGVKNADDGTPCTDNNACTDPDLCMAGVCEPGPDVCGEG"
     gene            2409..2564
                     /locus_tag="VHM69_08420"
     CDS             2409..2564
                     /locus_tag="VHM69_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740456.1"
                     /translation="MTDCATQPMRFETDTALALEAAFDGGRITSDGGLVWLAEADEEL
                     GLCEAIS"
     gene            2568..3734
                     /locus_tag="VHM69_08425"
     CDS             2568..3734
                     /locus_tag="VHM69_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX2740457.1"
                     /translation="MPEWRKRKGRHSLASLVRQRVFQIACGYEDQNDSDSLREDPLLK
                     MVCGSLPESGQDLASQPTFSRLENAADVGCCYRIARALFELYLRERAKDGAAPKRVLL
                     DFDATADPTHGDQEGSYYHGYYEQHIYHPLLVFDGETGHLIGALLRAGNTHASNSAVA
                     LLKRIVGGLRDEWPAVAIELRADAGFAIPALYDYCEEEGITYTVGLVTNPRLEEIARD
                     LLDEATERHETGGEKVRIFSEDTYRAGSWEHARRVVYKAEAMEQGTNTRFVVSTRADA
                     PEDLYEWYVRRGESENWIKDLKLALKADRLSCHRFIANQFRLLLHAAAYWLMDALRRR
                     LVAAGVRRMQLDTLRLVLIKVGGRVRQLLTKVRLHLASGHPGQLLWNALVNNPG"
BASE COUNT          697 a         1179 c         1199 g          673 t
ORIGIN      
        1 cctgatctct aaaggcgact gcgcaattgc gaacttggtc catcctcaaa gaggagagac
       61 cgctgagaag attgcagcgc ctttggctca gatcaagacg gatctcatcc tatgcaccga
      121 gtcaccgaag agagctggct caccaggagt gacgctctta tagtcaagtc ctacgcgacg
      181 tcagctccta cgaagcccct ctcgctccgg attggcgatg gctccacggt ggtcgaggag
      241 agcaggacac ggtagacctc cctggcgatg taccgtttaa ggcacctcat gatctcccgc
      301 ttgctcttcc cctccgccgt gcgcctcgcg acgtaggagc gggtgcgtcg atcggagccc
      361 attctgacga cgcagatcat gtgcagcgcc ctgttggcgt cccggttgcc gccacggttg
      421 agccgatgcc ggaccacttt gcccgaggat gcatcgacag gcgagactcc gcagaggctg
      481 gcgaaagaag cttcgctgcc caggcgctcg gggttgtccc ctgccacaac caggagtgtc
      541 gcggcgtgat gcgtaccgat tgccggcagg gagatcagtc ccggagcagc ctccgccact
      601 agtcgatcca gttgcgcgtc cagctcggcg atctcctccg agaggacctc atgccggcgg
      661 gccatcgaac gaagcgcgaa cctggttgcc gtctccacgc cgtctggatc attaccgggg
      721 cggaaacgag cacaggtagc gacaagctcg cttgtcgaga gcccgcacag gcggcgctga
      781 agcctctctg gtgccgttac ccgtaaggct tgtagctggt tggcggcctg gcttctggcc
      841 ttcaccgccg aacagcgggc cgctctgagg acccggatca tctccacctg accatcagcg
      901 ctctttggtt ctccggccgc ctcgccggcc aggaccgccc tcgcggcggc ttcggcgtct
      961 ttagggtcgg acttgccgtt gcgcttgagg tggcgccgtt tgggcctctc gacctccatc
     1021 actgtaatgc ccgtggactt caggtatcgg gccagcccgg caccgtagct gccggtcccc
     1081 tccacgcccg cgcacctcac gggcccgaac ccctccgccc agcagaggag gctgcggtag
     1141 cctccggtgg tcgtcggtac cttcgactcg cccaggcgcc tgcccaggtg gtcgaggacc
     1201 atggccccgt ggaagtccag gtgggtgtcc acacccaaca ccaccccctc gaccacggtc
     1261 gccgaaccct ctgcgcagta ttcgtcgccg gtcgtcttca cgctcatcgc ccctttcggt
     1321 gtcgcgggca ctgccggccc gaggaggcgg agagcggacg atactgtgac gggtcaggtc
     1381 acggcgagct cttattaggt cacgaagctc cggtctcacg ggcagttgcg gagcggcgcc
     1441 ccagatcgac cgacgggtca ctctcaaggc accttcgggc cggcggatgg atgggtcagg
     1501 tcggtccgag gcgtcgccca caaccaccta tagcgtattc acagcggatg gaagcatagc
     1561 gcgaagggct cggattgctg cgctcagcca ggacctctag ggccgccaag tcggccttga
     1621 caaaatgtgc cttctcatgg aagttcgccc aatcaaaatg ctcggaagtt gcgccgggtc
     1681 ttgtaacccc tcatacttgc cttcgttggt caacggggcg gggcttcccc gagtcccgcc
     1741 cttcggggaa aagggggcaa cgggtgagcc aacagactac caaccgcacc ttcgacgagc
     1801 tggctagagg gctggctagc ggctccatca cgcgaggtaa ggcgctcaag ctgatgggag
     1861 cagccctcct ggggggcacg ctagcatcgc ttggcatccg tgaggctgcc gccgacgagg
     1921 cgtgtaagcc cctcaacaag aagtgtagga aggacgagca gtgctgtagc ggcctctgcg
     1981 aaggcggcac gtgcgctcct gcgtgccagg tcgacagcga ttgcccagac cccggcactc
     2041 agtgccagca ggcggcctgc aacaacggcg tctgcggcgt caagaacgcg gacgacggca
     2101 ccccgtgcac cgataataac gcgtgcaccg accccgactt gtgcatggct ggggtgtgtg
     2161 agccggggcc agatgtgtgc ggcgaggggt agtacctatc agggcctagg cgcgatctaa
     2221 tcaccgtacc cagctccccg tataagcgga gttaacaggg ccgggcatcg tagctccggc
     2281 cctccttcag gcccttagtc acccgactca cctagcccgg attattcacg aaggtagttg
     2341 aagagaggga gccttctggg ctatggtcgg gttgttcgaa gactcgaccc agcaatagga
     2401 ggctcccgat gacggattgt gccacacaac cgatgcgttt tgagacggat acggcgctcg
     2461 cccttgaagc cgccttcgac ggggggcgca tcacctcaga cggcgggctc gtgtggctgg
     2521 ccgaggcgga cgaggagttg ggtttgtgcg aggccatctc ctagtgcgtg ccggagtggc
     2581 gaaagaggaa agggcgccac tcgctcgcct cgctcgttag gcagcgcgtt ttccagatcg
     2641 cttgcggcta cgaggatcag aacgactccg actccttgcg agaagacccc ttgctcaaga
     2701 tggtttgcgg ctctttgccc gagagcggtc aggacctggc cagccagcct accttctcgc
     2761 gcctggagaa cgccgcggac gtcggctgct gttaccgaat agcccgagcg ctcttcgagc
     2821 tctacctgag ggagcgagcc aaagacggcg ctgctccgaa gagggtgctt ctggacttcg
     2881 atgccacagc ggaccccacc cacggggatc aggaaggctc ctactaccat ggctactacg
     2941 agcagcacat ctaccatccc ctgctcgtct tcgacggcga gacgggacac ctcatcggcg
     3001 cgctgttgag ggcgggcaac acccatgcgt ccaactcggc ggtggccctg ttgaagcgca
     3061 tcgttggtgg cttgagggac gaatggcccg ctgtcgccat cgagctaagg gcggacgcgg
     3121 gattcgccat tccggccctc tacgactact gcgaagagga gggcatcacc tacacggtgg
     3181 ggctcgtaac caacccccgc ctggaggaga tcgcccgcga tctgctcgat gaggccacag
     3241 agcgccacga aacaggaggc gagaaggtga ggatcttttc cgaagatacc taccgggccg
     3301 gaagctggga gcatgctcgc agggtcgtct acaaggccga ggcgatggag caagggacca
     3361 acacccgctt cgtggtgagc acgagggcag atgcgcccga agatctctac gaatggtacg
     3421 tgaggcgggg agagagcgag aactggatca aggatttgaa gctggcactc aaagccgatc
     3481 ggttgagctg tcatcggttc atcgcgaacc agttcaggtt gttgttgcac gcggcggcgt
     3541 attggctcat ggacgcctta cgcaggaggc tcgtggcggc gggtgttcgg cggatgcaac
     3601 tagacaccct gaggttggtg ctcataaagg tcggcggcag ggtgcgtcaa ctgctcacga
     3661 aagtgcggct tcacctcgcc tctgggcacc ccggacagct tctctggaac gctctcgtga
     3721 ataatccggg ctaggcgtct agcaccac
//
LOCUS       DATAIR010000345         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_241082, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000345 DATAIR010000000
VERSION     DATAIR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241082"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VHM69_08430"
     CDS             complement(<1..1630)
                     /locus_tag="VHM69_08430"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX2740458.1"
                     /translation="MEIYAPLAHRLGIYSIKWELEDLSFATLHPRRYEEIKSLVAARR
                     GDREAFINGTAAELLRHLRETGIKAEVHGRVKHFYSIYNKMVRRNKEFNEIYDLAGLR
                     VVVDNVRDCYGALGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLLEIQI
                     RTAEMDTTAEFGIAAHWMYKHGLTDRQVNRLTWLKSMMEWQKETTDSTEFMESLKGEL
                     VADEVFVFTPKGDVVSLPSGATPIDFAYHVHTEIGHRTIGARVNDRIVPLDSELVSGD
                     RVEIITGKNSGPSRDWLAVVQSGRARNKIRQFFNKADREDNLSLGREKLLGLLKRRRI
                     GKVPSGILEDVARSTNHSSPDDMLAAIGAGVLSAENVAGRIVEQVRPKEEEETPKKQS
                     PALAPLPIPESGTDEETGVRVVGSSGILTRLARCCTPMPGDEIVGYVSLGRGVVVHSA
                     GCVNARALQARDPDRFVEVEWARGSDKLFTVELLVEALDRMHLLKDITATISDAGVNI
                     VSARVDTIEDRTALSRFAFKAASVQHVEEVIRKIR"
BASE COUNT          254 a          543 c          497 g          354 t
ORIGIN      
        1 tacggatctt gcgtataacc tcctcgacgt gctggacgct cgcagccttg aacgcgaacc
       61 ggctgagtgc cgttctgtcc tcgatggtgt cgacccgcgc ggagacgatg ttcacaccgg
      121 cgtcggagat cgtcgcagtt atgtccttga gcaggtgcat cctgtcgagg gcctcgacga
      181 gcagctcgac ggtgaagagt ttgtcggatc ctcttgccca ctcgacctcg acgaacctgt
      241 cggggtcgcg cgcctgcagc gcgcgggcgt tcacacagcc agccgagtgg accaccaccc
      301 cgcgcccgag ggagacgtat ccaacgatct cgtcacctgg catcggcgtg cagcaccggg
      361 ccagtcgcgt caggatgccg cttgaaccca cgacgcgcac ccccgtctcc tcgtccgtcc
      421 cgctctcggg gatcggtagc ggggcgaggg ccgggctctg cttcttcggg gtttcctctt
      481 cctccttcgg cctgacctgc tccacgatgc ggccagccac gttctcggcg gagagcacgc
      541 cggccccgat ggcggcgagc atgtcgtcgg gggaagagtg gttggtcgag cgcgccacgt
      601 cttccaggat gcccgagggg accttcccta tccttcgcct cttcaggaga ccgaggagct
      661 tctcacgccc gaggctcagg ttgtcctccc tgtcggcctt gttgaagaac tgcctgatct
      721 tattgcgcgc ccgcccgctc tggaccactg cgagccagtc cctgctcggt cccgagttct
      781 tgcctgtgat gatctccacc ctgtcgcccg agacgagctc ggagtcgagg ggaactatgc
      841 ggtcgttgac cctggcaccg atggtcctgt gcccgatctc ggtgtggacg tggtaggcga
      901 agtctatggg cgtcgccccg ctcgggaggc tgaccacgtc gcccttcggc gtgaacacga
      961 agacctcgtc ggcgacgagc tcgcccttca acgactccat gaactcggtc gagtcagttg
     1021 tctccttctg ccactccatc atgctcttga gccacgtcag acggtttacc tgcctgtccg
     1081 tcaggccgtg cttgtacatc cagtgtgccg cgatgccgaa ctccgctgtc gtgtccatct
     1141 cggcggtacg gatctggatc tccagcagct tcccttcgtt ggacatgacc gtcgtgtgca
     1201 gcgactggta catgttgaac ttgggcatgg cgatgtagtc cttgaaccgg cctgggatgg
     1261 gcttccagat cgaatgtatg acgccgagcg caccgtagca atcacgcacg ttatcgacca
     1321 cgaccctgag tccggccaga tcgtagatct cgttgaactc cttgttgcgg cgcaccatct
     1381 tgttgtagat ggagtagaag tgcttgaccc tgccgtgcac ctcggccttt atccccgtct
     1441 cgcgcaggtg cctgagcagc tccgccgccg tcccgttgat gaacgcctcc ctgtcacccc
     1501 gcctggcggc gacgaggctc tttatctcct cgtagcgccg cgggtggagg gttgcgaatg
     1561 agaggtcttc gagctcccac ttgatggagt agatccccag acgatgggcg agaggagcgt
     1621 agatctccaa cgtctccgtc gccttctt
//
LOCUS       DATAIR010000346         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_22383, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000346 DATAIR010000000
VERSION     DATAIR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22383"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1436
                     /gene="hydA"
                     /locus_tag="VHM69_08435"
     CDS             30..1436
                     /gene="hydA"
                     /locus_tag="VHM69_08435"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX2740459.1"
                     /translation="MARTLFSGGTVVTAEGSFRADVLVEDEKVVAVGTNLDANGAETV
                     DASGKLVMPGFIDAHTHMDMPFGGTVTADDWATGTESAAAGGTTMIIDFALQEAGGTL
                     AEAFDTWTEKARDKAVIDYSFHVAITDLRDDIKAELPDLAQKGVASVKIFMAYKGTPL
                     YTEDEDLFEALQLSKGAGVLVMVHAENGDAIAKLQEQALARGDTAPRFHALTRPEPVE
                     AEATNRAIRLAEVAEAPILVVHVSCAPALEAIHLAHERGQTVYAETCPQYFAFEYEDL
                     AREGFEGAKYVCSPPLRDPRNRPALWNGLKFGDLQIFGSDHCSFNYEGQKEMGRDDFT
                     LIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQARIYGAYPRKGTLAPGADA
                     DIVLWDPELSTTATIDNRHGNVDYTPYEGMEFHGGPEAVYVRGGLVYKDGEVVGERGS
                     GRFVERSFTATEGLEVRV"
BASE COUNT          311 a          466 c          490 g          242 t
ORIGIN      
        1 gcttccggtc tacgaaagga gtagaagcta tggcaaggac actttttagc gggggaactg
       61 tcgtaaccgc agagggcagc ttcagggccg acgttctcgt cgaggacgag aaggtagtcg
      121 ccgtaggcac gaacctcgac gcgaacggcg ccgaaactgt tgacgcttcc ggaaaactgg
      181 ttatgcctgg ctttattgac gcacacaccc acatggacat gcccttcggg ggcaccgtca
      241 cggcggatga ctgggccacg ggtaccgagt cagcggcggc gggcggaact accatgatca
      301 tcgacttcgc cctgcaagag gccggcggca cactcgcaga agcattcgat acgtggaccg
      361 agaaggcgcg ggacaaggcg gtcatagatt acagcttcca cgtcgccatc actgacctgc
      421 gcgacgatat aaaggccgaa ttgccggacc tggcacagaa aggcgtggca tcggtcaaga
      481 tcttcatggc ctacaaaggc acacccctct acaccgagga cgaagacctc ttcgaggcac
      541 tgcagctatc taaaggcgcc ggagtcctgg tcatggtcca cgcggagaac ggagacgcga
      601 tcgccaagct ccaggagcag gccctcgcgc gcggcgacac ggccccccgt ttccacgccc
      661 tcacccgccc cgagcccgtc gaggccgagg cgacgaaccg ggcgatcagg ctggccgagg
      721 tcgcggaagc gccgatactc gtggttcacg tctcgtgcgc gcctgcgctg gaggccatcc
      781 acctcgccca cgagcgcggc cagacggtct acgctgagac ctgtccccag tacttcgcct
      841 tcgagtatga agacctcgcc agagaaggct tcgagggcgc gaagtacgtg tgttctccgc
      901 cgttgcgaga tcctcgcaac cggcccgcgc tgtggaacgg cttgaagttc ggggatttgc
      961 agatcttcgg ctcagaccac tgctctttca actacgaggg ccagaaggag atgggccgcg
     1021 acgatttcac cctgatcccc aacggactgc cgggcgccga agaacgggcg atgacgctct
     1081 ggacgctcgg cgtgcgcgaa gggaagctct cggagaacca gttcgtcgcc gtcctctcca
     1141 cgaatcaggc gcgcatctac ggcgcctatc cgagaaaggg gaccctggcc ccgggcgccg
     1201 acgcggacat agtgctctgg gaccccgaac tctccacgac ggccaccatc gataaccgtc
     1261 acggcaacgt ggactacacg ccgtacgagg ggatggagtt tcacggcggt cctgaggccg
     1321 tgtacgtgcg gggcgggctg gtctacaaag atggtgaagt cgtcggcgag cggggctcgg
     1381 gcaggttcgt cgagcgcagc ttcacggcga cggagggact ggaggtgcgc gtatgacgaa
     1441 cccggaggag cttttgcagc ggcagaggga cgtgttgccc gattggatca cactctacta
     1501 cgagaagcc
//
LOCUS       DATAIR010000347         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_299713, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000347 DATAIR010000000
VERSION     DATAIR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299713"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..647)
                     /locus_tag="VHM69_08440"
     CDS             complement(81..647)
                     /locus_tag="VHM69_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740460.1"
                     /translation="MKFFETSTGNLVDAEAAAGVGHHVALSVVGTERLSESGYFRAKI
                     AQEKRINRSSIKYSIVRATQFFEFIERIADDATDGNAVRLAPVLIQPMAAEDVASAVG
                     RICVGSPVNGIVEVAGPQEFHLDELVRRFLRERRDPREVITDPHPRYFGAELDERTLV
                     PGDDARLAETRFEDWLSQAGVYGSSRKG"
     gene            complement(1008..1364)
                     /locus_tag="VHM69_08445"
     CDS             complement(1008..1364)
                     /locus_tag="VHM69_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX2740461.1"
                     /translation="MAHSDEERAEGGRRMETTGQSYSSGEWLVRAGSEEEFIQRWTTF
                     IEWSMDNAAGAESFVLVRSMEEPRKFLSLGAWESQQAQEAWREMPRMQVMLGHCRALC
                     DEFDTHRYTLAASRGR"
     gene            complement(1601..1738)
                     /locus_tag="VHM69_08450"
     CDS             complement(1601..1738)
                     /locus_tag="VHM69_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740462.1"
                     /translation="MLATILLLGGLLRGRMMHEHQIAAQHRNYRRQDYDRCTEARAAA
                     R"
     gene            complement(1822..2379)
                     /locus_tag="VHM69_08455"
     CDS             complement(1822..2379)
                     /locus_tag="VHM69_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740463.1"
                     /translation="MGNGWSVALAVALAVALGDAGVVGTTVTGGAVAVALGDPAGEAA
                     VPSAVAVGEAVVPSGEAVTVEVGSGCVGSGVIVAEPAAVCDALADAVAETLGEIVVVG
                     SGTGHPPSSVVSAVAEEVAVEVGSTVVPSAVAVAVGVAVVVDSGEPLGQPSGGAGIMS
                     LGWRLRCAWYRTEFRSTVIWTYLPP"
     gene            2372..2797
                     /locus_tag="VHM69_08460"
     CDS             2372..2797
                     /locus_tag="VHM69_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740464.1"
                     /translation="MPNEPFIPTPTAIATNLGENPDGPFEVTDPKGKVSTLSFYDLDV
                     HDDPPLPGGPGGITRSEMRGQRAAAQSLAEGGDFHEAVAAAQPFLNACDSRDAVRLAA
                     VQLGIPMPETGGPASLGIIAALLLMGSGIFAWRLGMRGR"
     gene            3144..3545
                     /locus_tag="VHM69_08465"
     CDS             3144..3545
                     /locus_tag="VHM69_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740465.1"
                     /translation="MTKVRSSVWVLSLLVLVGFGAGCGSASDQARQEAKKKAENKAQQ
                     IHKEAKQKVEAKKQQAKENVEAKKQELNEKVDDLEKKVNDLKKEVTELQKKINAQEQK
                     EQDQQINQLKKALKELEKKVETHQQDHQEKN"
BASE COUNT          816 a         1121 c         1108 g          622 t
ORIGIN      
        1 aacctgaggt gagtaaaggt ttcaggctac aggcatcagc aaaaagacct gtagcctgat
       61 gccttctccg aaggagtagc tcatcctttt cgagaactgc catagacccc cgcttggctg
      121 agccagtcct cgaagcgggt ctcggcgagt ctggcgtcgt cgccgggcac gagtgtgcgc
      181 tcgtccagtt ctgcgccgaa gtagcgcggg tgcggatcgg tgatcacttc gcgtggatcg
      241 cggcgttctc tgaggaatcg tcggacgagt tcgtcgagat ggaactcttg tggccccgcc
      301 acttcaacga tgccgttgac cggcgatcct acgcagatcc tgccaaccgc actggcgaca
      361 tcctcagccg ccatgggttg gatcagaacg ggtgcgagtc gcaccgcatt gccgtccgtc
      421 gcatcgtcgg cgattctctc gatgaactcg aagaactgcg tagcgcggac gatcgagtac
      481 ttgatagacg atctattgat ccgtttctcc tgggcgatct tagcacggaa gtagccactt
      541 tcggagaggc gttcggttcc cacgaccgac aacgcgacgt gatggcccac gcctgccgcc
      601 gcttcggcat caacgaggtt gccggtcgat gtttcgaaga acttcaacac tgccgcgtcc
      661 tccaaagatg ggagttcgac acatctacga ccacagcggc actgtcgagg acttcggcca
      721 agccctcgcc ggtgagcgtg ttgacgcccg aggctggtga cgccggcacc gcttggtggc
      781 cgtgctcaag aagcgtgcgg acgagctttt agccgatgag tccgcttccg tcgataacca
      841 cgattttcat ggtgagcctc cttttctact ccggagccta caggagaacg tagtgaggag
      901 ctatgccgag cgaagtagtg aagaaatcgc tcaagcgaac gtcgcatgcg aacttcgtag
      961 aagtgcctag aaggactagg gtgcttctcc ctagctcacc ttatctacta tcggccccgg
     1021 ctggccgcca aggtgtatcg gtgggtgtcg aactcgtcac acagcgcccg gcagtggcca
     1081 agcatcactt gcatccgggg catctctcgc cacgcctcct gcgcctgctg gctctcccac
     1141 gctcccaaag agaggaactt cctaggctct tccatgctcc gaaccaaaac gaaggactcg
     1201 gcccccgctg cgttatccat cgaccactcg atgaaggtag tccacctctg gatgaactcc
     1261 tcctcgcttc ctgccctaac caaccattca cccgagctgt aggactgccc tgttgtctcc
     1321 atccgtcgtc ctccttcggc ccgctcctca tctgagtggg ccatatacct ctccaccact
     1381 catctagctg cttcccagcg gatcccttac cctagtacat gatgccaccg gagcagtgca
     1441 ggtgcgaagt tcgcagaatt cgactgcccc gacgttgggt gaatagacgg tcttaggggc
     1501 ctgtccctat caaattcatc gggaattcat cgcctctggc tattattgct gcgtacgtag
     1561 ccgcaaaagg aaggacgcct ctatgaaccc gtccgaattc ttatcgcgcg gcggcgcgcg
     1621 cctcagtgca acggtcgtaa tcctggcggc gatagttgcg atgttgggct gctatctggt
     1681 gctcgtgcat catgcgtccg cgcagcaagc cgcccaagag cagtatggtg gcgagcaagg
     1741 gactccggct gcggagcaag ggactccggc agcggagcag ctccccgcgg acaacgtcac
     1801 catctgtcac tgggctgaag gtcagggtgg caagtatgtc cagatcacgg tggaccggaa
     1861 ctctgtgcga taccaggcgc acctcaaccg ccatcccaac gacataattc ctgccccgcc
     1921 cgagggctgc ccgagcggct cgcccgaatc gaccacgaca gccacgccaa cagcgacggc
     1981 cacggctgag ggcacaacgg tcgagcctac ctccacagcc acctcctctg ccacagcgga
     2041 gactacggac gatgggggat gtcctgtgcc cgagccgacc accacgatct cgccgagcgt
     2101 ctcagcgacc gcatcggcga gcgcatcgca gaccgcagcg ggctcggcga cgatcacacc
     2161 ggagcccacg caaccggagc caacctctac ggtgacagcc tcgccggagg gcaccacagc
     2221 ctctccaacg gccacggcgg agggcaccgc agcctcccca gcaggatcac ctagtgccac
     2281 ggcgacagcc ccgccggtta cggtggtacc gaccacgccg gcatcgccga gtgccaccgc
     2341 gagcgcgacg gcgagcgcca cggaccaacc gttgcccaac gaaccattca tccccacccc
     2401 aaccgccatc gcgacaaacc tgggagagaa ccccgacggc ccgttcgagg tgacagaccc
     2461 caaaggaaag gtgagcaccc tatcgttcta cgacctcgac gtgcacgacg acccgccgct
     2521 tccggggggt cccgggggga tcacccgctc cgagatgcgg gggcagcggg cagccgcgca
     2581 gtcgctcgcg gagggtggcg acttccacga ggcggtcgct gccgcacaac cgttcctgaa
     2641 cgcgtgcgac tcgcgtgacg cggtgaggct tgcggcggtc cagcttggga taccgatgcc
     2701 tgagacgggt gggcctgcat cacttgggat catagcggcg ctgctgctga tgggcagcgg
     2761 catcttcgcg tggcggctgg gcatgcgagg cagatagtaa gagaggcgaa catcttgcgg
     2821 cggagagatg tgtcagccgt gcgcgaagtt ccgagaacgc gaagttcgca gaacgctcct
     2881 ccttccacgc actccggtga ataggatgct gcgttatcgc cgtcggcaag aagtcatcgc
     2941 tagttcatcg ccgtccgaca gtatgagaac atgggacgcg cgccaagcca tgtgtggtgg
     3001 atagtactca gaggcgcaca tcgatgcctc tggagaacac ccggcaatgg agcgaacgcg
     3061 cagaagaagt cgtggagatc gtcgggacgt aaagcaagaa aagccacaat agccgagaga
     3121 cgaaggagag gacggttcca gaaatgacga aggttcggtc gtcggtatgg gtactgtcct
     3181 tgctagtgct cgtggggttc ggggcaggat gcgggtcggc ttccgaccag gcccggcagg
     3241 aggcgaagaa gaaagccgag aacaaagctc agcagatcca caaagaagcg aagcaaaagg
     3301 tcgaggccaa aaagcaacag gcgaaggaga acgtagaagc caaaaagcaa gagctaaatg
     3361 agaaggtaga cgatctggag aagaaggtaa acgacctcaa gaaggaagta accgagctgc
     3421 agaagaagat aaacgcccaa gagcagaagg aacaggatca gcagataaac caactgaaaa
     3481 aggcgctaaa ggagctggag aagaaggtag agacccacca gcaggaccac caggagaaga
     3541 actagacgca cgactcgtca gggagcgcac cctacgcagt cgggtgaata agggtagcgc
     3601 gaggcttagc ttgcgaggtc gtcgaagccg caacgcctgg cctcttgaag tgagagcgag
     3661 gccgtgt
//
LOCUS       DATAIR010000348         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_115106, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000348 DATAIR010000000
VERSION     DATAIR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115106"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VHM69_08470"
     CDS             <1..432
                     /locus_tag="VHM69_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740466.1"
                     /translation="VQKLGRAPTESELVMRLGISITEHRAFLNQYARAQVRSLEARPD
                     AQGGPYGGLHDLIGDHAATDPESAAEVAEVRARLVRAIQGLGEQERVVTTFYYYEGLT
                     LREIGRALNLTEGRISQILHRALMRLRESLSEESRLSEGAW"
     gene            631..1707
                     /gene="rpsB"
                     /locus_tag="VHM69_08475"
     CDS             631..1707
                     /gene="rpsB"
                     /locus_tag="VHM69_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2740467.1"
                     /translation="MQSTTERVGIRELLEAGVHFGHQAKRWNPKMKRYIFAQRAGVHI
                     IDLDQTLTLLNKALDFVRGVAEANREVLFVGTKKQAQIPLAEQALRSGQPFVAERYIG
                     GMLTNFQTILPRIQYFKDLAVRVDETPEEDRTGKDWFALNREYQKLRRNFAGITEMER
                     LPGAVFVIDPKREELLVKEANRLKIPVLALTDTNCDPEVIDYVIPGNDDAIRAISLIS
                     RLVADAIIEGRGEETPVEERAVPPVIEDARVNAEVQQAGTTNGRTETSDAAPTVDAPP
                     VAESPGNGETREEPEATVDTETSVEQETPVEPEATADAEAASEPEPEVETQPEPEAAA
                     EPEPEAPDTDEEAPDNDSEETEEK"
     gene            1713..2300
                     /gene="tsf"
                     /locus_tag="VHM69_08480"
     CDS             1713..2300
                     /gene="tsf"
                     /locus_tag="VHM69_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2740468.1"
                     /translation="MASTIEKIKQLREETAAGMMDVKRALEESDGDVEGARKVLREKG
                     QAMAAKKSGRAATEGLIEAYVHFNGRVGVLVEVNCETDFVARTPEFKEFARNVALHVA
                     SMRPLVVSPEDIPAGVLEEERQVVEKQVAEMGKPENIAQKVVEGRMNKWVAEQALLTQ
                     PYVKETDKTVGDLLQETIQKVGENVVIRRFVHYEL"
BASE COUNT          525 a          707 c          796 g          335 t
ORIGIN      
        1 gtgcagaaac tcggccgcgc gccgacggag tccgagctgg tcatgcgcct cggtatcagc
       61 atcaccgagc accgtgcgtt cctgaaccag tacgcgcggg cacaggtcag gtcgctagaa
      121 gcccgcccgg atgctcaggg aggaccatat ggcgggttgc acgatctcat cggggaccat
      181 gccgcgaccg accccgagtc ggcggcggag gtggccgagg ttcgtgctcg gctggttagg
      241 gccatacagg gtctcggtga gcaggagcgc gtggtcacca ccttctacta ctacgagggc
      301 ctcacgctca gggagatcgg gagggccctc aacctcacgg agggccgcat ctcgcagata
      361 ctccacaggg cgctcatgcg gctcagggag agcctctcgg aggagagccg gctctccgaa
      421 ggcgcatggt aagacagctc gctagaagca tccatgccct tgtgctaaca tctgcggggc
      481 cggacaaggc gtcaagtcac gtcttgcctg cgattacaca cgcccgccgt agggtcctcc
      541 ggacccgggt ggtggtccgt cggagctcag gaggcggatt gcgaggcgcg ggcggaggat
      601 accaaccacc tgaaaagaga ggtagagatc ttgcagagca caaccgagcg cgtaggaatt
      661 cgagagctgc tggaagcagg cgtccacttc ggacaccagg ccaagcgctg gaacccgaag
      721 atgaaacgct acatcttcgc ccagcgcgcg ggggtccaca taatcgacct cgaccagacg
      781 ctgaccctac tcaacaaggc cctcgacttc gtccgcggcg tcgcggaggc gaaccgcgaa
      841 gtcctcttcg taggaaccaa gaagcaggcc cagatcccgc tcgccgaaca ggcgctgagg
      901 agcgggcagc ccttcgtggc ggagcgttac ataggcggca tgctgaccaa cttccagacg
      961 atactgccgc gcatccagta cttcaaagac ctagccgtca gggtcgatga gacccctgag
     1021 gaagacagga cgggcaaaga ttggttcgcc ctgaacaggg agtaccagaa gctcaggcgc
     1081 aacttcgccg gcataaccga gatggagcgc cttccaggcg ctgtcttcgt catagacccg
     1141 aagcgtgagg agctcctggt aaaagaggcc aaccggctca agatccccgt cctcgccctt
     1201 acagatacca actgcgaccc cgaggtcatt gactacgtca tccccggcaa cgacgacgcg
     1261 atacgggcga tcagcctgat ctccaggctc gtcgctgacg cgattatcga gggcaggggc
     1321 gaagaaactc cggtggagga acgtgctgtg ccgccggtga tcgaagacgc cagggtgaac
     1381 gcggaggtcc agcaggccgg gacgacgaac ggccgtacag aaacgtcgga cgctgccccc
     1441 accgtagatg cgccgccagt ggccgaatcc cccgggaacg gcgagactcg ggaggaacca
     1501 gaagctacgg tagataccga gacctcggtg gagcaggaga cccccgtcga gccggaggcc
     1561 accgctgacg ccgaggctgc ctccgaaccg gagccagagg tagaaacaca gcctgaaccg
     1621 gaggccgctg cggagcctga acctgaggcg cccgacactg atgaggaggc gccggacaat
     1681 gatagcgaag aaaccgagga gaagtaggga ctttggctag tacgatagag aagataaagc
     1741 agctccggga ggagactgcc gcgggaatga tggacgtgaa gcgggcgctg gaggaatccg
     1801 acggtgacgt cgagggcgcc cggaaggtcc tgagggagaa gggccaggcc atggccgcca
     1861 agaagagcgg gcgggcggcg accgaagggc tcatcgaggc ttacgtccac ttcaacgggc
     1921 gggtcggcgt gctcgtcgag gtcaactgcg agacggactt cgtggcccgc acgccagagt
     1981 tcaaggagtt cgcccgcaac gttgccctgc acgtcgcctc catgcgaccg ctggtcgtct
     2041 cgccggagga catccctgcc ggggtgctcg aagaagagcg tcaggtcgtc gagaagcagg
     2101 tcgccgagat gggcaagccc gagaacatcg cgcagaaggt cgtcgaaggc aggatgaaca
     2161 agtgggtcgc ggagcaggct cttctcacgc agccctacgt caaagagacg gacaagacgg
     2221 tcggtgacct ccttcaggag accatccaaa aggtcggcga gaacgtcgtg atccggcgct
     2281 tcgtccacta cgagctctag tagaggcgtg gaagagtccg ccagatattc gggcgagacc
     2341 aatggggccg ggctctcgga gct
//
LOCUS       DATAIR010000349         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_115185, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000349 DATAIR010000000
VERSION     DATAIR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115185"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VHM69_08485"
     CDS             complement(<1..327)
                     /locus_tag="VHM69_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2740469.1"
                     /translation="MNGTPALALEGVHKSFGENEVLKGIDLTVAPHEVVCLIGASGSG
                     KSTLLRCVNLVEPIDSGRVVVDGEEITAEGVDANRIRRRIGIVYQSFNLFPHMTVLRN
                     VTLAPRE"
     gene            complement(324..1184)
                     /locus_tag="VHM69_08490"
     CDS             complement(324..1184)
                     /locus_tag="VHM69_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2740470.1"
                     /translation="MTTRGTGPSGRGSRAFGNRAVLVSSASTVLFFALITVVVVLAPG
                     SEVVAERFFSPQHLWQSLVGSEGEPSVLRAFLLNVKIFMVSEVFILILALVIAVVRGI
                     PGPAFFPFRLVAVAYTDLFRGVPLILVLYMIGFGVPGLGLGFISYLPDVTYGIIALVL
                     VYSAYVAEVYRAGIESVHESQNAAARSLGLTRWQSLRFVVLPQAIRRVIPPLLNDFIG
                     LQKDTALVSVLGSIEAARAAQIYGASEFNYASYVVAAFLFVLITIPLARFTDRLISRD
                     KRRREAGALA"
     gene            complement(1186..2010)
                     /locus_tag="VHM69_08495"
     CDS             complement(1186..2010)
                     /locus_tag="VHM69_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2740471.1"
                     /translation="MRERGILVGLLSVVLLLVGCGGPSAESGSTDSCDMDNPPLYEEG
                     GLTVATDKPAYPPWFKGDPQNYSGYEGEIASEIAQRMNLPLKWTVEPFNKSYAPGAKD
                     FDFDINEITITPEREEVVDFSDGYFDNAQGVLVMKDSPAADAESVSDLKDLTIGAQVG
                     TTSLDFINETVKPATDPKVYDSTNDAKSALESGQIDALVTDLVTTVYLRDFEIEGSTV
                     VGQYPRNEQFGMLFEQGNPLVDCVNQVLGEMKSDGTLKKLQQKHLQQYLDVPPLEK"
     gene            2227..3021
                     /locus_tag="VHM69_08500"
     CDS             2227..3021
                     /locus_tag="VHM69_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX2740472.1"
                     /translation="MSESEQQWDDSPLLSERGATKISNTVVSSLAGMAAREVEGVHMG
                     GGASRTASGVLGSITGSESKTSGVSVEVGNTEVAIDLKMGIEYNKNILQTVEEVRRRI
                     TDRVQSMTGLRISELNATITDITFPEKEQRRRIGSGAATKELGEGSSSETESDTRTMP
                     SSEPRPVASEETTQVETGGPADAESTSEPDSQVDETAELRTDDTETEEQPASVESESG
                     ATSETETPEEPETRAEAEPSEETEAEARRRRRAEERARRRRRTEDG"
     gene            complement(3143..3691)
                     /locus_tag="VHM69_08505"
     CDS             complement(3143..3691)
                     /locus_tag="VHM69_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2740473.1"
                     /translation="MAVKEFHNETPTTVDGERKSPVTTNATVPMRIRSSVPSRRVLRI
                     INPFVSVILRSPLHRLLSSQVLLLTYTGRKTEKRFTFPVFYTHEGDTLILFSSRSWWK
                     NLRRGGNVAMHLQSRARTGRAEVIEERSAVLDAAEHLVAKYGLKEAGRRIGLALDISP
                     PPTTDELAAAMEDRVVIRIILD"
     gene            complement(3806..>4001)
                     /locus_tag="VHM69_08510"
     CDS             complement(3806..>4001)
                     /locus_tag="VHM69_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740474.1"
                     /translation="LLVAMLMHASLTASTLILGPLAISGVALLTYSLALAAATWVVVA
                     AVAVAQGGHLSRRPLLRRVA"
BASE COUNT          787 a         1189 c         1315 g          710 t
ORIGIN      
        1 ctcgcgcggg gccagggtga cgttgcgcag gaccgtcatg tgggggaaga ggttgaagga
       61 ctggtagacg atgcctatgc ggcgcctgat gcggttcgca tcgacgcctt cggctgtgat
      121 ctcctcccca tctaccacga cacggcctga atcaatcggc tcgacgaggt tgacgcatct
      181 cagaagagtg gacttgcccg agccggaggc cccgatcagg cataccacct cgtgcggcgc
      241 gaccgtcagg tctatgccct tcagtacctc gttctccccg aaggacttgt ggacaccttc
      301 gagggcgagg gctggagttc cgttcatgcc agggcccccg cctcccggcg gcgtttgtcg
      361 cgggagatca ggcggtccgt aaagcgggcg agggggatgg tgatcaggac gaagaggaag
      421 gccgcgacta cgtaactggc gtagttgaac tcggaggcgc cgtagatctg ggcggcccgc
      481 gccgcctcta tggagccgag gaccgagacg agcgcagtgt ccttctgcaa ccctatgaag
      541 tcgttgagga gcggcggtat gacgcgcctt atcgcctgtg gcaggaccac gaaccgcaaa
      601 gactgccagc gcgtgagccc gagcgagcgg gccgcggcgt tctggctctc gtgtacggac
      661 tcgatacccg ccctgtagac ctcggcgacg tacgccgagt agacgagtac gagggcgatg
      721 atgccgtagg tcacgtccgg gaggtacgag atgaagccta atcccaggcc aggcactccg
      781 aacccgatca tgtacaggac aaggatcagt ggcactcccc tgaatagatc cgtgtaagct
      841 acggcgacaa gcctgaaagg gaagaacgcg ggccctggta tcccgcgcac cacggctatg
      901 acgagggcaa ggatcaggat aaagacctcg gagaccatga agatcttcac gttgaggagg
      961 aacgcgcgga ggacagaggg ctcgccctcc gagccgacga gcgattgcca gaggtgctgc
     1021 gggctgaaga accgctcggc cacgacctcg ctccccggcg caagcacgac gactacggtg
     1081 atgagcgcga agaagagcac cgtgctcgcg ctcgatacta gcactgcccg gttcccgaag
     1141 gcccgcgagc cccggcctga agggccggtc cccctggtcg tcacgctact tctctagagg
     1201 cgggacgtcc aggtactgtt gcaggtgctt ctgctggagt ttcttcaagg tcccgtcgct
     1261 tttcatctcg ccgaggacct ggttcacgca gtcgacgagc gggttgccct gctcgaagag
     1321 catcccgaac tgctcgttgc gcgggtattg gccgacaacg gtagagccct ctatctcgaa
     1381 gtcacgcagg tataccgtgg taaccaggtc ggtgacgagg gcgtcgatct ggccgctctc
     1441 cagcgcggac ttcgcgtcgt tggtggagtc gtagaccttg ggatcggtcg ccggcttcac
     1501 ggtctcgttg atgaagtcga ggctcgtggt cccgacctgg gctcctatgg tcaggtcctt
     1561 gaggtcggag acgctctcgg cgtccgccgc tgggctatcc ttcatcacga gcacgccctg
     1621 ggcgttgtcg aagtagccgt cggagaagtc gacgacctcc tcgcgctccg gggttatggt
     1681 gatctcgttg atgtcgaagt cgaagtcctt ggcgcctggc gcgtaagact tgttgaaagg
     1741 ctccacggtc cacttgagcg ggaggttcat gcgttgggcg atctcgctcg ctatctctcc
     1801 ctcgtagccc gagtagttct gcgggtctcc tttgaaccac ggcgggtagg cgggtttgtc
     1861 cgtggcgacc gtcagcccgc cctcctcgta gagcggcggg ttgtccatgt cacacgaatc
     1921 agtagaaccc gactccgccg agggtccgcc gcagccgacg agcaacagga ccacggagag
     1981 gagcccgacc aggatacccc gctctctcac acgcctctct ccgatctact ctccgaatga
     2041 aaagcgatgg ccgacattgc agagatatcc cggaccatca aagcaagtat agggtccgtc
     2101 cgaatgcgct gcaatcgacg tgcttctccc cgagaagatc ttctggatct tcggatactg
     2161 gtatcctggt tgctcgtggg ctgttcgggg aacagttaac aaaaccgtcg acggaggaga
     2221 gtaagtatga gcgaatcaga gcagcaatgg gacgacagcc cgctcctgag cgagcgcggt
     2281 gcgacgaaga tcagcaacac ggtcgtctcc tccttagctg gcatggcggc gcgggaggtg
     2341 gagggcgtcc acatgggagg gggcgcatcg aggaccgcaa gcggcgtact ggggagcata
     2401 acgggatcgg agagcaagac cagcggcgtc tccgtcgagg tcggcaatac ggaggtcgcc
     2461 atagacctca agatggggat cgagtacaac aagaacatcc tccagaccgt cgaggaggtg
     2521 cggcgcagga tcaccgaccg agtccaatcg atgacggggc tcaggatctc cgagctgaac
     2581 gcgacgatta cggacatcac cttccccgag aaggagcaga gacgccgcat cgggtcaggt
     2641 gccgccacga aagaactcgg cgaaggctcg tcgagcgaga cggaatctga tacgcggacg
     2701 atgccgagta gcgagcctcg tcccgtcgcc agcgaggaga ctacgcaggt agagaccggc
     2761 ggcccggcag atgccgagag cacgagcgag ccggactccc aggtcgacga gacagcggag
     2821 ctgcgtaccg acgataccga aaccgaggaa cagcctgcga gcgtggagtc ggaatcaggc
     2881 gcaacttcgg agacggaaac accggaggag ccagagacac gcgcagaagc agaaccgtcc
     2941 gaggaaacgg aagcagaggc gcggcggagg cggcgcgcgg aggagcgtgc caggcgtcgc
     3001 aggcgcaccg aagacggcta ggctcgggga gcacgccagg cgtgaatcag agcgtgcgag
     3061 tgcattagcc tcggccttag ggggcgtgtt tagcgcatgt tatcaggggt gacctttctc
     3121 agccgggatg cacgctgttt cctcagtcta ggatgatgcg gatcaccacg cgatcctcca
     3181 tcgccgcggc cagttcgtct gtggtcggtg gagggctgat gtcgagcgcc aggccgatcc
     3241 tccttccagc ctccttgaga ccgtacttcg caacaaggtg ctcggctgcg tccagcactg
     3301 ccgaccgctc ctcgatcacc tcagcgcggc ctgttcgcgc gcggctttga aggtgcatcg
     3361 ctacgttgcc gccccttcgc aggttcttcc accaggaacg gctggagaag aggatgagcg
     3421 tatcgccctc atgggtgtag aagacaggga aggtgaaccg cttttccgtc ttgcggccgg
     3481 tgtaggtcaa cagcagcacc tgactgctca gcagtcggtg gagcggtgag cgcaggatca
     3541 cggacacgaa cgggttgatg atccgcagga ctcgcctact cgggacgctc gaccttatcc
     3601 gcatagggac ggttgcattg gtcgtcacgg gactcttcct ctccccatcg accgtcgttg
     3661 gcgtttcgtt gtgaaactct ttgaccgcca tttctttcac ctctctgttg tcgggaggga
     3721 cccctttctt gaggaagctc cgcaccctga ttggctatct ttgtggtctt tactagttgg
     3781 agatcccttg agcgctcccc ctttctcaag ccacgcgcct taggagcggc cgtcgcgaga
     3841 gatgcccgcc ttgggccacg gcgacagccg caacgacgac ccacgtcgcg gcggccagtg
     3901 cgagggagta ggtcaagagg gccacccctg atatcgccag aggaccgagg atcagcgtgc
     3961 tggccgtgag gctcgcgtgc atgagcatcg ctacgagcag a
//
LOCUS       DATAIR010000350         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_56366, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000350 DATAIR010000000
VERSION     DATAIR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56366"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VHM69_08515"
     CDS             <1..481
                     /locus_tag="VHM69_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2740475.1"
                     /translation="RGQLDLQGSYAYTAEDFEQALSLGATAVHGSGEETREALEEPTE
                     GLGADLVIDAVGAEVTRRMALDLLQPAGRVVYVGLHDDATTLGFHDIVRGQLDLQGSY
                     AYTAEDFEQALEWLVEGRAGIGELPPVLPLEEGPGAFAELVRGPSAQIKVFLAEAGE"
     gene            481..1224
                     /locus_tag="VHM69_08520"
     CDS             481..1224
                     /locus_tag="VHM69_08520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2740476.1"
                     /translation="MGDLSGKTALVIGASSGIGLATANLFADEGAEVHAAARRREAIE
                     EGAGGREVTAHDLDISDRESVLRTVEEVGEADGIDILVVAAGMNFPERRLEQLTADRW
                     DAMISVNLSGAFYAIRAALPFLRASQGLVVLISSVSGRWPDASGPAYQASKAGMTELA
                     HASGFEEHVNGVRFTSILPGIVDTPILDNRPEPPPKEVRDAALKPEDVAQACLFLATL
                     PPRAYVPELTMVPTAIQALGKTSTATPPV"
     gene            1390..>2025
                     /locus_tag="VHM69_08525"
     CDS             1390..>2025
                     /locus_tag="VHM69_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2740477.1"
                     /translation="MRRRKRIEEALKESEERYRAVVEQSVEAIYLYDAETKQVLESNA
                     AFGQMIGYTEEELLGMRIYDFIAHDVDDIDLNVRRSLEAGRRYIGERKYRRKDGSVMI
                     VDTSASVISYGGKTALCAVSRDVTERRKFEEALKKSEERFRSLVQNASDLITILEADG
                     TVRYDSPAIERMLGYGLEERLGTSAFDYIHPEDVSRVRRTFSKTLDGQGVQP"
BASE COUNT          397 a          610 c          682 g          336 t
ORIGIN      
        1 gcggggtcaa ctcgacttgc agggatcgta cgcgtacacg gcagaggact tcgagcaggc
       61 gctctccctc ggcgcaaccg ccgttcacgg gtcaggcgag gagacgcgcg aggcgttaga
      121 ggaaccgacg gaaggattag gtgccgacct ggtcatcgac gccgttggag ctgaagtcac
      181 gcgccggatg gccctggacc tcctgcagcc agccggacgc gtcgtgtatg tcgggttgca
      241 cgacgacgca acgaccctgg ggttccacga catcgtgcgg ggtcaactcg acttgcaggg
      301 atcgtacgcg tacacggcag aggacttcga gcaggcgctc gaatggctcg tcgagggcag
      361 ggccggtatc ggggagctac cgcctgtgtt gccgttggag gagggccccg gggcgttcgc
      421 cgagctggtg cgcggtccat cagcgcagat caaagtcttc ctcgcggagg cgggggagtg
      481 atgggggacc tctcggggaa gacggcgctc gtgatcggcg cgagcagcgg tatcggtctc
      541 gccactgcca acctcttcgc cgatgagggc gcggaggtcc acgccgcggc caggcgccgc
      601 gaggcgatcg aggaaggcgc gggtggacgc gaagttacgg cccacgatct cgacatctcg
      661 gatagagagt ccgtcttgcg gaccgtcgag gaggtcggag aggctgatgg aatagacatc
      721 ctcgtcgtcg cggccggtat gaacttccca gagcggcggc tggagcagct cacggccgac
      781 aggtgggacg ccatgatctc ggtaaacctc agcggcgctt tctatgcgat ccgggccgcc
      841 ctccctttcc tgcgtgcctc gcagggtctc gtcgtcttga tcagcagcgt ctccggacgg
      901 tggcccgacg cctcggggcc ggcctaccag gcttcgaagg ccgggatgac cgagctggcg
      961 cacgcctctg gtttcgagga gcacgtaaac ggcgtgcgct tcacctcgat cctgcctggc
     1021 atcgtggaca cgcccatcct ggacaaccgc cccgagccgc ccccgaagga ggtcagggac
     1081 gcggccctca agcccgagga cgtggcgcag gcttgcctct tccttgccac tctcccgccc
     1141 cgcgcctacg tcccagagct caccatggtc ccgaccgcga tacaggctct gggcaagacc
     1201 tcgaccgcga cgccgccggt atagcttgcg cgcgtttctg atgacttgtc cgtgaatgtt
     1261 accaatgtag tatgctgtgg acatagattg aaagggagtc ctggaggcga cacagaaccc
     1321 caggctcagt cgaaaggtta cctaattgct cgacccgttg acgatccctt cgatggtgag
     1381 aaataagctg tgagaaggcg gaagcggatc gaggaggcgc tcaaggagag cgaggagcgc
     1441 tacagggccg tcgtcgagca gagcgtggag gcaatctacc tctacgacgc cgagaccaag
     1501 caagtacttg aatccaacgc cgccttcggt cagatgatcg gctacaccga agaagagctc
     1561 ttggggatgc gcatttacga cttcatcgcc cacgacgtgg acgacatcga cctgaacgta
     1621 aggagaagtc tggaggcggg gcggcgctac atcggcgaga ggaagtacag gcgcaaagac
     1681 ggctcggtga tgatcgtgga cacgagcgcc tccgtaatct cttacggcgg caagacggcc
     1741 ctgtgcgcgg tctcgcgcga tgttacggag cgcaggaagt tcgaggaggc gctcaagaag
     1801 agcgaggagc ggttcagatc tctcgtccag aacgcctcgg accttatcac gatcctcgaa
     1861 gcagacggca ccgtacgcta cgatagccct gccatcgagc gcatgctcgg ctacgggctc
     1921 gaagagcggt tgggaacgag cgccttcgat tacatacacc cggaagacgt ctcgcgcgtc
     1981 agacgcactt tcagcaagac cctggacggt caaggggtcc aaccg
//
LOCUS       DATAIR010000351         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_22616, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000351 DATAIR010000000
VERSION     DATAIR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22616"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VHM69_08530"
     CDS             <1..336
                     /locus_tag="VHM69_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740478.1"
                     /translation="AGHAEIFCNQVQYHPYRNQDALLGQAGEMDYLLTAYTPLSRGGV
                     QEDTTLREIGEAHGKTATQVALRWLVQQDNVCAIPKATSDEHLMENLDIFDFDLSREE
                     MLSISSLSR"
     gene            346..1482
                     /locus_tag="VHM69_08535"
     CDS             346..1482
                     /locus_tag="VHM69_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2740479.1"
                     /translation="MRARDLGIKIGDLDSGPHNAITDVDGVRVGHSTIVRGEGRLVVG
                     EGPVRTGVTVVCPREGLARDEPVFSGSHRFNGNGEMTGLEWIREAGALATPVAITNTH
                     SVGVVRDALVTAEMEARVEEEDYWCLPVVAETYDGILNDINGQHVTADHVREALADAT
                     GGPVAEGSVGGGTGMICHEFKGGIGTASRRLPEEKGGWTVGALVQSNYGRRSMLRVDG
                     APVGRVLSTVRVPSPYEEEELPAGTGSIIVVLATDAPLLPTQCDRLAKRAGIGLGRMG
                     GGLDDGSGDIFLAFATGNRGIPRAGLHRPPSTVSLAMVSNDYMTPLFHAAAEATEEAI
                     LNALLAAGDMTGRDGIKAHGLTQELLLGALDEARAMCARQDVET"
     gene            1505..>2436
                     /locus_tag="VHM69_08540"
     CDS             1505..>2436
                     /locus_tag="VHM69_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX2740480.1"
                     /translation="MVDGERLWRRISELGEIGRHESGGVTRLSLTDEERAAKDMVADY
                     MEEAGLSVREDAAGNLFGRREGRDPDAPAVIVGSHVDSVYEGGNFDGPLGVLSGIEIV
                     QTMEERGIETEHPVEVVAFTDEEGARFSFGMIGSRALAGSLSTEDLSHEDENGVTIAD
                     AMRDYGLDPERVLDAARPAGSVAAYVELHIEQGRVLENEGLPVGVVTGIAAPVWRKFV
                     FTGETGHAGTTPMGHRSDALAAAAEVMAVIEAEASATGSSVGTVGQLGVEPGGINIIP
                     GRVELSLDLRDIDEETRDLVEGRILQRTREVCEKR"
BASE COUNT          448 a          751 c          849 g          388 t
ORIGIN      
        1 gcggggcacg cggagatctt ctgcaaccag gttcaatacc atccttacag gaatcaggac
       61 gcgctcctcg gccaggccgg ggagatggac taccttctga ccgcctacac ccctctctcc
      121 cgcggcggcg ttcaggagga caccaccctc agagagatag gggaggccca cggcaagacg
      181 gcgacgcagg ttgccctgcg ctggctcgtc cagcaggaca acgtttgtgc catccccaag
      241 gcgacgagcg atgagcacct catggaaaac ctcgacatct tcgacttcga tctctccagg
      301 gaggagatgc tgtctatctc ctccctgagt cgctgaggcc tccacgtgcg cgcccgtgac
      361 ctcggaataa agatcggtga tctggattca ggacctcaca atgcgatcac cgacgtcgat
      421 ggcgttaggg tcgggcactc gacgatcgtg cgcggggagg ggcggctcgt ggtcggtgag
      481 ggacccgtca ggacgggcgt tacggtcgtc tgtccccgcg aggggctggc gcgtgacgag
      541 ccggtcttct caggttctca caggttcaac ggcaacgggg agatgacggg gctggagtgg
      601 atcagggagg ccggcgccct tgcgacgccc gtcgcgatca ccaacaccca cagcgtcggt
      661 gtggtacgcg acgccctggt aacggccgag atggaggccc gcgtagaaga ggaggattac
      721 tggtgcctcc ccgtggtcgc cgagacctac gacggcatcc tgaacgacat caacggccag
      781 cacgttaccg cggaccacgt gcgcgaagct ctcgctgatg cgaccggagg accggtggca
      841 gaaggctccg tcggcggggg gacgggtatg atctgccacg agttcaaggg cggtataggc
      901 acggcctcgc gccggctccc ggaagagaag ggcggctgga cggtcggggc cctcgtgcag
      961 tccaactacg gacgccgctc catgctgcgt gtggacggag cacccgtagg gcgtgtcctg
     1021 agtactgtgc gcgtcccttc gccttacgag gaggaggaac tgccggcggg gacgggttct
     1081 atcatcgtgg tcctagccac ggacgccccg cttctgccga cacagtgcga ccgtctcgcg
     1141 aagcgcgccg gcattggcct cggcaggatg gggggcggcc tcgacgacgg gagcggcgac
     1201 atcttcctcg ccttcgccac gggcaaccgc ggcatcccac gcgccggact acaccggcca
     1261 ccttcgaccg tgtccctggc gatggtctca aacgactaca tgacgccgct tttccacgcc
     1321 gcggcggagg cgactgagga ggccatcctg aacgcgctcc tggcggcagg agacatgacg
     1381 ggccgcgacg gcatcaaggc ccacggtctc acgcaggagc tcctgctcgg cgcgcttgac
     1441 gaggcccgcg caatgtgtgc gcgtcaggac gtggagacct gaagaacccg gaggtgacac
     1501 gaacgtggta gacggcgaac ggttatggcg caggatctcg gagctaggtg agatcggacg
     1561 tcatgagtct ggcggcgtga cgaggctctc gctcacggac gaagaacgag cggccaaaga
     1621 catggtcgcc gactacatgg aagaggccgg cctctctgtc cgcgaggacg ctgcggggaa
     1681 tctctttggc cggcgtgagg gaagggaccc cgacgccccc gccgtcatcg ttgggtctca
     1741 cgtcgactcg gtatacgaag gcggcaactt cgatgggccc ctcggagtgc tttcaggtat
     1801 cgagatcgtg cagacgatgg aggagcgtgg catagagacg gagcatcccg tcgaggtcgt
     1861 cgcgttcacc gacgaagaag gggctcgctt ctctttcggg atgatcggaa gccgcgcgct
     1921 tgcgggcagt ctctccaccg aggacctgtc ccacgaggac gagaacggcg taactatagc
     1981 cgatgcgatg cgcgattacg ggctggaccc ggagagggtc ctggatgcgg cgcgtcccgc
     2041 tggttccgtc gcggcctacg tcgagctgca catagagcag ggccgggtcc tcgagaacga
     2101 gggcctgcct gtcggggtcg tcacgggtat agcagccccc gtttggcgaa agttcgtctt
     2161 cacgggagag accgggcacg caggcacgac gccgatggga catcgcagcg acgcgctcgc
     2221 cgctgcggcc gaagtcatgg ccgtcatcga ggccgaggcg tcggctacag gctcatccgt
     2281 gggaacggtc gggcaacttg gcgtcgaacc gggaggtatc aacatcatcc ctggccgcgt
     2341 cgagctctca ctcgacctca gggacataga cgaggagacg cgcgaccttg tcgaggggcg
     2401 catcctgcag cgcacgcggg aagtgtgcga gaagcg
//
LOCUS       DATAIR010000352         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_241229, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000352 DATAIR010000000
VERSION     DATAIR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241229"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..295)
                     /locus_tag="VHM69_08545"
                     /pseudo
     CDS             complement(106..295)
                     /locus_tag="VHM69_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(522..1493)
                     /gene="pfkA"
                     /locus_tag="VHM69_08550"
     CDS             complement(522..1493)
                     /gene="pfkA"
                     /locus_tag="VHM69_08550"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2740481.1"
                     /translation="MRRIAVLTSGGDAPGMNAAIRAVVRTGIDKGWEVLGVRRGYAGL
                     IAGDAVPLGARDVGGIIQQGGTILSTARSPEFKTEQGRRRAIQQLERLGVEALVVIGG
                     NGSQSGAHALSQAGVPVVGVASTIDNDLHGSEITIGVDTALNVALEAIDRLKITASSL
                     QRAFLVEVMGREFGYLAMMAGITGGAEAIVVPEVETHPDEVAAELQAAYERGKAHALV
                     VVAEGAHYNARAIERHFAEYRGRIGFELRVTILGHVQRGGTPTFSDRLLATRLGAAAT
                     EHLATGEHGVMIGTMGSEIVATPLAEVVAKKKVLDVELMELAGVLVK"
     gene            complement(1548..1883)
                     /locus_tag="VHM69_08555"
     CDS             complement(1548..1883)
                     /locus_tag="VHM69_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740482.1"
                     /translation="MMERTGGERVEVLVGVVLHGEFHGKRLRFTGKEVDRYDTGESRH
                     VLYECGPDRYRILVSRFDDGSEIYPEPEMGDESSGISYGFLTTEQAAARWPHKEHFAR
                     SYPLEDIDV"
     gene            complement(1928..2410)
                     /locus_tag="VHM69_08560"
     CDS             complement(1928..2410)
                     /locus_tag="VHM69_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2740483.1"
                     /translation="MTCKERLEAYLRENGVPYQSQHHARAITAQEVAATEHVPGRMFA
                     KTVIVLPDDEANMVMLVLPAPYHVNPLKASAALRANDVHLADEERFADAFPDCDVGAM
                     PPFGNLYDVPVYTDRTLVEDETIVFRAGTHTDTMSVSYADFEKLVRPTVADFADPYGV
                     "
     gene            complement(2459..3352)
                     /locus_tag="VHM69_08565"
     CDS             complement(2459..3352)
                     /locus_tag="VHM69_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2740484.1"
                     /translation="MSIFPTKVLLATDGSEEAVLAATTASGIGSRTGSELHVLTVGPE
                     YPYYELPDYPAYFEELVAAQRREAKEVLDEQVRKIEESGGAVEEAHLEMGIPPGRAIV
                     DLAEKLGAGLIVLGSRGLGPMRRSLMGSVSSSVVHHAHCPVLVVRGEGHDFPPGRILL
                     ALDGSAESSSAARTAVDLADGLESELHLVHVGDVKPAYHPERHGYHALHVKIRERAQR
                     LLDEQVNEVRSAGGTVAKAHLRMGRPDEEIVVLGEEIAAGLIVTGSRGLGGVRRALMG
                     SVSDSVVRHAHCPVMVVRRET"
BASE COUNT          578 a         1148 c          988 g          702 t
ORIGIN      
        1 gacggatcga gatcggagac taggagcccc ggcacatgga ggtcgcagac ctcttacata
       61 tgtctactcg aagtagtcct cagctttggt gatgtaggct tccatatcgt cagggacttc
      121 atcctcgggg tagatggcct cgaccggaca ttccggctcg caagccccgc agtcgatgca
      181 ctcctcaggg tttatgagga actggtctcc ggcatcgtag atgcagtcca caggacacct
      241 ccacgcaaga ctggtctttc gtgccgatac acggctcagt gatcacatag gtcaatggct
      301 ctcctctccc ggcctacctt agggtagttt acacccgtcc ggcgaagcct tgcatacttc
      361 gtttacgata ttgtcccgac gcatgaaaca cgtcgcgatg gctggtgacc cgtagctgag
      421 gcacgctgta ggggccattg aacgaagccc tggttgactc cttcacggcg gtagtgcatt
      481 cgtgcgtgcg aactgacttt ctaccgccga gcgtggcctc tctacttcac cagcactcct
      541 gccagctcca tcaactccac gtccagcacc ttcttctttg cgaccacctc ggccagcggc
      601 gtggccacta tctccgagcc catcgtcccg atcatcacac cgtgctcgcc cgtggcgaga
      661 tgttcggtag ccgctgcgcc gagcctggtg gcgagcaacc ggtctgagaa ggtgggtgtc
      721 ccgccacgct ggacgtgccc gaggatcgtg actcgcaact cgaagccgat cctgcccctg
      781 tactcggcga agtgccgctc gatcgccctt gcgttgtagt gggcaccctc ggcgaccacg
      841 accagggcgt gtgccttgcc acgctcgtag gcagcctgta gctctgcggc cacttcgtcg
      901 gggtgcgtct cgacctcggg gacgactatg gcctccgccc cgccggttat acccgccatc
      961 atagccaggt acccgaactc tcgtcccatc acttccacca ggaacgctcg ttgcagggag
     1021 gaggccgtta tcttcagcct gtcgatggct tccagcgcga cgttgagggc cgtgtcgacg
     1081 ccgatggtga tctccgaacc gtggaggtcg ttgtctatgg tcgaagccac gccgaccaca
     1141 ggaaccccgg cctgagagag cgcgtgggcg cctgactgcg agccgtttcc tccgatgacg
     1201 acaagggctt cgacgccgag gcgctcgagc tgctggatgg cccgacgacg cccttgctcg
     1261 gttttgaatt ccggagagcg tgcggtgcta aggatggtgc cgccctgctg aataatgccg
     1321 ccaacgtcgc gggccccgag cggcacggcg tcgcccgcga tgaggccggc gtaacctcgc
     1381 ctgacgccaa ggacctccca gcccttgtct atgccggtcc gaacgacggc cctgatcgcg
     1441 gcattcatgc cgggagcgtc tcccccgctg gtcaacacgg cgatgcgcct cacgttgcct
     1501 ccgggttcgt gcggcgggtc gatcgcctca gaatccgcac ggttcctcta tacatcaata
     1561 tcttccaggg ggtaagagcg ggcgaagtgc tccttgtgcg gccatctcgc ggccgcctgt
     1621 tccgtggtga gaaacccgta ggagatgccg ctgctctcgt ctcccatctc tggttcaggg
     1681 tagatctcgc tgccatcatc gaagcgggat acgagtatcc tgtagcggtc aggcccacac
     1741 tcgtagagca cgtgcctgga ttcgccggtg tcgtacctgt ctacctcctt gcccgtaaac
     1801 cggagcctct tgccgtggaa ctcgccgtgg aggaccacgc ccaccaggac ctcgaccctc
     1861 tcaccacccg tgcgctccat catcgctcct cctcatcgcg aacttcaacg cggagacgct
     1921 tgactcctca tacgccataa gggtctgcga aatcggcgac ggttggcctg acgagtttct
     1981 cgaaatctgc gtaggagacg ctcattgtgt cggtatgggt gcccgccctg aagacaatgg
     2041 tctcgtcctc aacgagggtt ctgtcagtgt agacaggtac gtcgtagagg ttaccgaagg
     2101 gcggcatcgc gccgacatcg caatcgggga aagcgtccgc gaagcgttcc tcatcggcaa
     2161 gatggacgtc atttgcacgc agcgccgccg acgccttgag agggtttaca tgatagggcg
     2221 cgggcaacac cagcattacc atgttcgcct cgtcgtctgg caggactatc accgtcttgg
     2281 cgaacatcct gccggggacg tgttcggttg ccgcgacttc ctgggcggtt atcgccctgg
     2341 cgtggtgctg gctctggtag ggaaccccgt tctcacgcag gtaggcctcg agcctctctt
     2401 tgcacgtcat cctacttctc ctccttcatg tccacgcccc gagctacagg ccgccacctt
     2461 acgtctcacg gcggaccacc atgacggggc agtgggcgtg acgaaccacc gagtcggaga
     2521 cgctgcccat gagcgccctc cttaccccgc caaggcccct gctcccggtt acgatgagtc
     2581 ccgctgctat ctcctcgcca agtacgacga tctcttcgtc cggacgtccc atcctgagat
     2641 gcgccttcgc gacggttccc cccgctgacc tgacctcgtt cacctgctcg tcgagaagcc
     2701 gttgggcgcg ttcccggatc ttcacatgca gggcgtgata tccgtgcctc tcggggtggt
     2761 aggctggctt cacgtctccg acgtgcacaa ggtgaagctc cgattccaaa ccgtcggcga
     2821 gatctacggc cgttcgggcg gccgacgacg actccgcgga gccgtcgagg gcgagcagga
     2881 tcctgccagg cgggaagtcg tgcccttccc cccgcaccac caggaccggg cagtgggcgt
     2941 ggtggacgac gctactggag acgctgccca ttaacgaacg cctcattggg ccgagccccc
     3001 tgctgcccaa gacgatgagc cccgctccca gcttttcggc aaggtcaacg atagcccggc
     3061 ccggcggtat gcccatctcg aggtgggcct cttcgacagc gcctcccgac tcctcgatct
     3121 tcctcacctg ttcgtccagc acctccttcg cctcgcgcct ctgcgcggct actaactcct
     3181 cgaagtaggc ggggtaatcg ggcagctcgt agtagggata ctcgggaccc actgtgagca
     3241 cgtgcagttc ggaccctgtt ctactgccga tgcccgaagc tgtggtggcg gccagcacag
     3301 cctcttcaga cccgtcggtc gccaacaaaa ccttcgtcgg gaatatgctc atgatctctc
     3361 ccttggtctc cgcactattt ctctgactac gcctcgaggg ctctcctggc ctctat
//
LOCUS       DATAIR010000353         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_194902, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000353 DATAIR010000000
VERSION     DATAIR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194902"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VHM69_08570"
     CDS             <1..430
                     /locus_tag="VHM69_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX2740485.1"
                     /translation="DFPYPQSGESGYRRRGVYSGGDRRRRRGLRRMIRASCHCGAVVL
                     EVDSPPVEVTDCHCSICRRYGTLWAYYAPERVRVLPSDPPTDVYVWGDRTLETHRCRE
                     CGCVSHWVAMDPGVDVMGVNARLMDPEVLSAARVRYSAGP"
     gene            complement(563..1045)
                     /locus_tag="VHM69_08575"
     CDS             complement(563..1045)
                     /locus_tag="VHM69_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2740486.1"
                     /translation="MRRIMLLLAVFTLTLLVASGVALAVTKIGTDGPDFLIGTKGSDE
                     LFGKAGTDWISGRGGDDVINGGPGSDDPLSRAVGMLDGGPGADVISGGPGQDYLDAGS
                     WPRDSAVDTLNGGDGNDFLHAENRPAARDIVSCGAGSRDGAVVDRKDIVADDCERIWR
                     "
     gene            1279..2091
                     /locus_tag="VHM69_08580"
     CDS             1279..2091
                     /locus_tag="VHM69_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2740487.1"
                     /translation="MVRQGYLVALAVAFLIGCAVLLIVVGASGVQAEASQEKKQGHSE
                     ATKEQEQSGGAASEADRCTGTRTIVRIGGTYLTNDLPGCPNKGGLLSAQLPDTGRNKI
                     SDPKYPLLMGENGDDKVRGLGGPNELYGGAGSDEVRGLGGLDDLFGGPGSDVIYGGSE
                     SPPSGDFVFGDKGDDVIYGGDGNDYVFGGKGADVIYGGDGKDSFEDSDGQRDKLYCGK
                     GKDEYVVDKIDYVDSSCEKGKLVGTGGPPLILLTAAALCSTLMMLRYVIRSA"
     gene            complement(2228..2782)
                     /locus_tag="VHM69_08585"
     CDS             complement(2228..2782)
                     /locus_tag="VHM69_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2740488.1"
                     /translation="MRKVVAAEFLSLDGVMESPDQWHFPYFNDEMGQAVGEGFAAADA
                     MLMGRVNYEEWAAFWPGQDPQENPMATQMNAMKKYVVSTTLEEPLGWQNSTLISENVA
                     DEVAKLKQGEGGDIVISGSGALVRSLLNYGLLDELKLMIHPILVGSGKRLFEEGEEQT
                     KLELVDSRTFSTGVLYLTYRPAQG"
     gene            complement(3024..3920)
                     /locus_tag="VHM69_08590"
     CDS             complement(3024..3920)
                     /locus_tag="VHM69_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2740489.1"
                     /translation="MKTKGVVAYLLLAFGLAWSTMFIVRLQLGLSLENSLVQIPIAFS
                     PAIAAVIVRRWITREGFGDAGLALHLRSSWAYYLAAWLFPLIAVGVAIGLATVFGVSR
                     PDLSQLGTLFPGVQLPGWAGLPLLMGVPILMMPLFWGEEFGWRGYLQIRLLSHSPLKA
                     AIATGFIWAVWHYPLYFLGYSEYANPYIGLITATVFAILLAIILAWLRLRTRSVWSSS
                     LAHAGTNMVLATLSTTLLVGGARIDWAVNDLLQSVPLAAMCAWIILSGQLRADRIPDQ
                     RSASTNSESAPQSLTQRPASPR"
     gene            complement(3935..4507)
                     /locus_tag="VHM69_08595"
     CDS             complement(3935..4507)
                     /locus_tag="VHM69_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEX2740490.1"
                     /translation="MDDAAERYLEELRRNLKPLPEDERSDAVREISSHIAEGQADGRP
                     TASVLAGLGDARTLARAYVADYHLREPSAGKLGSVARIVLSSAFVSGTSLLSLFVVPM
                     LALLTSFAGLMAIVTPVLGVLRTLGVPGIVMGDSGGWQVPILWSFPASLVLAAAFAVM
                     AWAGYKLLRGYLRLVLAGYRKLLPFRSDSR"
     gene            complement(4500..4859)
                     /locus_tag="VHM69_08600"
     CDS             complement(4500..4859)
                     /locus_tag="VHM69_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2740491.1"
                     /translation="MARVRAGEWSSQIRRGTLELCVLALIASKPRYGYDLVTALERWE
                     PLATTEGTVYPLLRRLQRDGKVEASWEESAEGPPRKYYRLTSHGRDLLGRMTADWAEL
                     SEAVRETLPAEEAESHG"
     gene            complement(5098..5544)
                     /locus_tag="VHM69_08605"
     CDS             complement(5098..5544)
                     /locus_tag="VHM69_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740492.1"
                     /translation="MDQPTAGEALKKLEPLVGEWILEAKPPDGPPWPGEARAKIEWHD
                     SGAHLVSRSTVDMPEAPDSISIMGCDAANGTYFQLYSDERGVCRVYEMSIGGGQWKLW
                     REGEPFPQRFSATISDDGNTIAGRWEKAPDGRTWETDFDLTYRKAT"
BASE COUNT         1177 a         1837 c         1758 g         1141 t
ORIGIN      
        1 ggatttccct tacccgcagt caggtgaatc ggggtaccgc aggcggggtg tatattccgg
       61 gggagaccgg cgtcgacgga gggggttgcg aaggatgata agggcttcat gccactgcgg
      121 tgccgtcgtc ctggaggtcg attccccgcc ggtggaggtg acggattgcc actgctcgat
      181 atgccggcgc tacggtaccc tgtgggctta ctacgcgccg gagcgggtgc gcgtgctccc
      241 ctcagacccg cccaccgacg tctacgtgtg gggcgacagg accctcgaga cccaccgctg
      301 tcgtgagtgc ggctgcgtct cgcactgggt cgccatggat cccggggtcg acgtgatggg
      361 cgtcaacgcg cgcctgatgg acccggaagt cctctccgct gcccgcgtga ggtactccgc
      421 cggtccctag ccgaactccg cgaagttccg agaacgccgt tagggcgacg ttcggagaat
      481 ccaccttcta ggtacttcgg tgaacaccgc atgaagaagg gcaggcgcgt agcagccccg
      541 gccctagcac tctgccccta atctatctcc atatcctctc gcagtcgtct gccacgatgt
      601 ccttgcggtc gactaccgct ccgtcacggc taccagcgcc acaactgacg atgtcgcgag
      661 cggccggacg gttttcggca tgtaagaagt cgttgccgtc accgccattg agggtgtcca
      721 cggcggagtc gcggggccac gaaccggcgt ctaaatagtc ctggcccggt ccacccgata
      781 tgacgtcggc gccagggccg ccgtccagca tgccgaccgc tcgtgataag gggtcgtcgc
      841 tacccggtcc gccatttatt acgtcatcac cgcctcggcc gctgatccag tcggtgcccg
      901 ccttgccgaa cagctcatcc gagcccttgg tacctatgag gaagtcgggg ccatcggtgc
      961 cgatcttggt gaccgccaga gcaaccccac ttgcaaccaa cagggttagg gtgaacacgg
     1021 ccaacaacaa catgatgcgc ctcattagcg caacctcctt gtgtagaggg cgggactcct
     1081 cagccccgcc tcaggttgta gagcgctacc acggtcgcaa acagcgatga atcgctaatg
     1141 aatccttggc gaccttcgga aagtcctgtt tccacgcact cgggtaaata agccttcctg
     1201 agagatgcga aaggcttctt ggtgtggcat gcttcgcggt attagtagca taggacacat
     1261 gcaaagggca ggtgtggcgt ggttcgtcaa ggatacctcg tagctctggc ggtagccttc
     1321 ctgatcggat gcgcggttct gctcatcgta gttggcgctt caggggtgca ggcggaagcc
     1381 tcgcaggaga agaagcaggg acacagcgag gccaccaagg agcaagagca atctggcgga
     1441 gcagcatcag aagcagaccg atgcacgggg acgcgaacca tcgtccggat tggtggaacc
     1501 tatctcacga atgacttgcc cgggtgcccc aacaagggag gcttgctctc cgctcagctc
     1561 cctgacaccg gcagaaataa gatttcagat ccgaaatatc ccctgctgat gggtgagaac
     1621 ggcgacgaca aggtacgcgg ccttggcgga cctaacgaac tctacggagg agcgggcagc
     1681 gacgaggtac gcggccttgg cggtcttgac gacctcttcg gaggaccggg cagcgacgtc
     1741 atctacggag gttcggagtc gccaccgagc ggagacttcg tattcggcga taagggcgat
     1801 gacgtcatct acgggggcga cggcaacgac tatgtgttcg gaggcaaggg cgcggatgtc
     1861 atctatggtg gggatggcaa ggattctttt gaggacagtg atgggcagag ggacaagctc
     1921 tattgcggta aaggcaagga cgaatacgtt gttgacaaga tcgactatgt ggacagcagt
     1981 tgcgagaagg gaaagctcgt cggtactggt ggccctccct tgatcctcct cactgctgcg
     2041 gcgctttgta gcacgcttat gatgctccgc tacgtgatcc gctccgccta gtgtaagctc
     2101 tcgctcctgc taggcgaact tctccgaacg ccgtaaagcg gaagtccgag aatttgaggt
     2161 tgggtgaata ggcaaccagg tcccgttcct atgggccagg aagtggtgct ccgaccttcc
     2221 aagagattca gccttgagcc ggccggtacg tgagatagag gacgcccgtg gagaaggtcc
     2281 tcgagtccac caactccagc ttcgtctgtt cctctccttc ctcaaaaagc cgcttgccgc
     2341 ttcccaccaa tatcggatgg atcatcaact tcagctcgtc gagcaacccg tagttcagga
     2401 gcgaacggac cagcgcgccg cttcctgaga ttacgatgtc cccgccctcc ccctgcttga
     2461 gcttcgctac ctcatcggca acgttctcgc tgatgagtgt agaattctgc cacccaagcg
     2521 gctcctcgag ggtcgtcgag accacgtact ttttcatggc gttcatctga gtggccatcg
     2581 gattctcctg gggatcctgc ccgggccaga aggcggccca ctcctcgtag ttcacccttc
     2641 ccatcagcat ggcgtccgcg gcggcaaagc cctcccctac cgcctggccc atctcgtcgt
     2701 tgaagtaggg aaagtgccac tgatcggggg actccatcac cccatctagc gacagaaact
     2761 ccgcagcaac cactttcctc atcttgatct gctcctccta cgtctcttgt ctcggctgac
     2821 cttagcatag agtcctgctt cgcaagtggc gaacgtctta cagcggcgtg cggaaggaat
     2881 gatcctcccc aagccgggct acaggcaaca ggtttcaggc atcaggaagg tcttcccgga
     2941 ccgacatcaa cctccaacgc actcggatga ataagggcaa aagaaggggt gaggcggctc
     3001 aggccccaga gagaggtacc ccgttatcgt gggctggcgg ggcgctgtgt caggctctga
     3061 ggagccgact cactgtttgt cgaggcggat cgttgatcgg ggatcctgtc cgctctaagc
     3121 tgtccgctaa ggatgatcca ggcgcacatg gccgcgagcg ggacgctctg gagcaggtca
     3181 ttgaccgccc aatctatcct cgcgcctcct accaacaggg tcgtagagag cgtggcgaga
     3241 accatgttgg ttccggcgtg tgcaagactg gaactccaca cgctcctggt gcgcaggcgt
     3301 agccaggcca aaatgatggc gagaagtatc gcgaagacgg tagcggttat cagtccgatg
     3361 taggggtttg cgtattctga gtaccccaag aaatagaggg ggtaatgcca gacggcccag
     3421 ataaaccctg tagcgatggc cgctttgagg ggactatgac tcagcaatcg gatctgcaag
     3481 tagcctctcc agccgaactc ttcgccccag aataagggca tcataaggat gggcactccc
     3541 ataaggagcg gcagccccgc ccagccgggg agttgcacgc cggggaagag ggtgccgagc
     3601 tgagaaagat ccgggcgtga gacgccaaac accgttgcca gcccgatagc cacgccaacg
     3661 gcgatcagcg gaaagagcca ggccgccaga tagtaggccc aactacttct gaggtggagc
     3721 gcgagtcccg cgtccccgaa tccttccctg gtgatccaac gccgcacgat caccgccgcg
     3781 atggcaggag agaaagcgat cggaatctgc accagcgagt tctccagcga caatcccaac
     3841 tgcagccgga caataaacat cgtagaccag gccagcccga aagcgagcaa tagatacgca
     3901 accacgcctt tggtcttcat cgatcgttgt gctcctaccg agagtcagag cggaacggca
     3961 agagcttgcg gtagcccgcc aggaccagcc gcaggtagcc acgcagcagc ttgtacccgg
     4021 cccatgccat gactgcgaag gcagcggcga gtaccaggga cgcagggaaa ctccacaaga
     4081 tcggtacctg ccagccccct gagtctccca tcacgatccc tggcaccccg agggtcctca
     4141 aaaccccaag aacaggagtc acgatagcca tcaggccagc aaaagaggtt agcagcgcca
     4201 gcatgggtac gacgaagagg ctcagcagac tcgttcccga tacgaacgcc gaggagagga
     4261 caatacgcgc caccgatccg agtttcccgg cgctcggctc cctgagatgg tagtccgcca
     4321 cgtaggccct ggccagtgtc ctcgcgtcgc ccagcccggc gagcacggac gccgtcggtc
     4381 gcccatccgc ttgcccttcg gcgatgtgac tcgagatctc tcgtaccgca tcagaacgtt
     4441 cgtcctcggg cagtggcttg aggttgcgac gcaactcctc aaggtaccgc tcggcagcat
     4501 catccatgac tctccgcctc ctctgcaggc aatgtttctc gcacggcttc gctcagctcg
     4561 gcccagtcgg ccgtcatacg ccccaagagg tcgcgcccat gcgacgtcag ccgatagtac
     4621 ttgcgcggag gtccctccgc ggactcctcc cacgaggcct caaccttgcc gtcccgctgc
     4681 aggcggcgca gcagagggta gacggtgccc tcagtggtcg ccagcggctc ccaccgttca
     4741 agggcggtca cgaggtcata accgtagcgc ggtttggagg cgatcaaggc caagacgcaa
     4801 agctcgaggg tgccgcgccg gatctgcgaa gaccactcac ccgccctcac gcgcgccacc
     4861 tccatatcgg acctgtgcga accttttcat aactacaagg taacacaagg tagtgtgtac
     4921 tgcaaggtag tgtgtcgagc gagcattcgg agtcccgcgg cacgatccgc cgtggaccct
     4981 tattcaccta acattgcggg aggcgcgtcc gtgcttccga gcatttccct ttcaacgcat
     5041 tcgggtgaat agggcatcgg ttatcgaagc actcgattat gcgcgtggtg aagccgccta
     5101 cgtggccttg cggtaggtga ggtcgaaatc ggtctcccac gtgcgtccgt ccggcgcctt
     5161 ctcccagcgg ccggcgatcg tgttgccgtc atcgctgatc gtcgcgctga agcgctgcgg
     5221 gaacggttcg ccctcgcgcc agagcttcca ctgcccgcct ccgatgctca tctcgtagac
     5281 gcggcagacg ccgcgctcgt cggagtagag ctggaagtag gtcccattgg ctgcatcgca
     5341 tcccatgatc gagatgctgt cgggtgcctc tggcatgtcc accgtcgagc gcgacaccag
     5401 gtgtgcgccc gagtcgtgcc attcgatctt cgcccgtgcc tcgcccggcc acggtggccc
     5461 gtccggcggc tttgcctcga ggatccactc gccgaccagc ggctcgagct ttttgagcgc
     5521 ctcccctgca gttggttggt ccatcgcgtt gctcctccct ccgattgctt gggaatcttt
     5581 tgcctacgag atacccgcat gttatccggc tggtctggca tcatacctcc cctggtccct
     5641 ttccctctat ggaggcgaaa cggtgcactt tggagcatcg tgcatgctga tctccgagaa
     5701 gcccatcaag gcgaaggtcg gagagtacgt acaccccgta ctgcagaatt tcctctttca
     5761 cacgctccgg cgaaagcggt tagaaatcta catttcccct gccaagtgcg tcgtcaagtg
     5821 cttgcccctt ccaccttctc gacatcttgc tcggtcagac tggaccggaa gttgatgtac
     5881 ggagtgtgtt gcctctcgtt aggctcatac tcg
//
LOCUS       DATAIR010000354         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_300033, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000354 DATAIR010000000
VERSION     DATAIR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300033"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VHM69_08610"
     CDS             <1..1714
                     /locus_tag="VHM69_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HEX2740493.1"
                     /translation="ESVLGLFLAQGMPQRMLRWHYRSRHESLITVSNHEFYDDRLTIF
                     PSPDRTGEDLGLVYHHLAGTVYDRGKTRTNTGEARAVAESVIRHAKENPRRTLGVVAF
                     SSSQAQAIEDQLELLRRGDPSCEEFFSRHPFEPFFVKSLENVQGDERDVMFISVGYGR
                     DANGNLTMVFGPLNQDGGERRLNVLITRARRRCEVFTNILPDDLDLGCTDARGVRSLK
                     IFLRFAREGHLEVPTASGRDPDSPFEEAVLRALRDLGYEVDPQVGSAGFFVDLAIKDP
                     EKPGRYLLGVECDGATYHSARSARDRDRLRQEVLEGLGWRIHRVWSTDWFSSPDSEMR
                     RLVEAIERARLHTAHEDIAPDNTATLPQARDTGEGPVERFERVETTGVALPKYETARP
                     NTSDYNFEVWDLHEVHLDDMSAWIAEVVWIESPVHLDDIVRRIAEACGVSRAGRRIRE
                     RVEAGCRRALKQGRARRSGDFFWLPEMDEAPMRDRSALPDSSKKPERIAPEEIGKAVE
                     KVVSESFGMRKEDIPPSVLKLLLGFRRATRGASATIMEVVERMLGDGKLSEEGDHVSV
                     ADSV"
     gene            complement(1804..2025)
                     /locus_tag="VHM69_08615"
     CDS             complement(1804..2025)
                     /locus_tag="VHM69_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2740494.1"
                     /translation="MQLSTRNRLPGTITEVVKGEAAAKITLQVGDNHLVALITRESAD
                     ELGLEPGKQATALVKATDVMILTDGGDIA"
     gene            complement(2006..2329)
                     /locus_tag="VHM69_08620"
     CDS             complement(2006..2329)
                     /locus_tag="VHM69_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2740495.1"
                     /translation="MDIDPLLRELAEGSSLESFRLLIEPLREQDRTRGSDLVRTLTTY
                     FAAGANASEAADRLFLHRNSMLYRLERIQNLTGLDLKDHRVALALQLGLLATERGERD
                     ATEHP"
     gene            2407..2742
                     /locus_tag="VHM69_08625"
     CDS             2407..2742
                     /locus_tag="VHM69_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2740496.1"
                     /translation="MNASRSDMFRKLLERDPRNPMVLYSLGNELFKEGHYGEARDHFK
                     RAIENKPDYSVAYRMLGRAHFELHEDTDARNIFMKGKEVARENGDLQTVREIDVFLRR
                     LEKREVGEG"
     gene            2769..3221
                     /locus_tag="VHM69_08630"
     CDS             2769..3221
                     /locus_tag="VHM69_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740497.1"
                     /translation="MSEANVGILVDYESPLARRVAGDVWSGLSRAALDAGFTKTTALW
                     ESLRETRSTMDLEGPLVHVFAIGQDRPDAIDAVSAIGDPGAPHLYGVVVAVPDRPSPL
                     AGSLLYQGVERLTGTGVKVGMVEPALLHAVPEECERYARHLLERLRIP"
     gene            3229..3924
                     /locus_tag="VHM69_08635"
     CDS             3229..3924
                     /locus_tag="VHM69_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEX2740498.1"
                     /translation="MAAQLLTALLVTAAFAGLAYALGMISHSGALGGLLVGTTIYVCL
                     GPQGFAVLALFVIGGSLLTRLGYRSKQRTGTAQEHGGRRGARNALANCAVATLCAVLA
                     ATTGSEILLAPFVASLGAAFADTTESEIGQLLSRTPRLITTLRKVPPGTDGAVSLPGT
                     LAGATAAGLTAALGLALGILETPEGALLVAIAAFLGTVADSLIGALSPRAGNELTNVL
                     CTLVAALLALIFN"
     gene            complement(3942..4385)
                     /locus_tag="VHM69_08640"
     CDS             complement(3942..4385)
                     /locus_tag="VHM69_08640"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2740499.1"
                     /translation="MPPIVHSIEIDRPPDEVFSYVTDPLRFAEWQYDVLSVRIEGGPP
                     PGVGSKFTTTRRIGRAERTMTQEITEINPPTSWAAQGVAGPIRPNANIIVDPLDDGTR
                     SRVTIALDFKGHGIGVPLVPAIRLMAAKGAPRSYRNLKERLESGE"
     gene            4468..5352
                     /gene="sigJ"
                     /locus_tag="VHM69_08645"
     CDS             4468..5352
                     /gene="sigJ"
                     /locus_tag="VHM69_08645"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX2740500.1"
                     /translation="MDDHDWLAEQFEENRTHLRAVAYRMLGSISEADDAVQEAWLRLS
                     RSDTSGIENLGGWLTRVVGRVCLDMLRSRRSRREEPMGARFPDPIVGREDGINPEHEA
                     LLSDSVGLALLVVLETLTPAERLAFVLHDMFGVPFEEIAPIVGRSPVASRKLASRARR
                     RVRGADLVPDSDLARQRELVDAFLAAARDGDLDALVAVLDPDVVLRADTRGVPLGAPR
                     EVRGAAAVAEQALNFSQRAPFAQRALVNGAAGVVVVSRGHPIAVMGFATIGGKIVEID
                     VLSNPARLRQLDLAILDD"
     gene            5479..6111
                     /locus_tag="VHM69_08650"
     CDS             5479..6111
                     /locus_tag="VHM69_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2740501.1"
                     /translation="MVTASEEPRARAKRDQILTGARRVFLRDGFSGASTDAIAAEAKV
                     SKRTLYVYYPSKEDLFVDVLRKLTIENPQTRALESMERMSPENREELSRDLLDLARKI
                     VATMMQPDYLALLRTTIADIHRFPQLAGLFRASVPERAMSSFAVFIERTRDRGVIRDD
                     VNGEAAARMFVGPLLTYAVLDGLFVAGGPPRKPAPGRIEQIVDMYVKAIT"
     gene            6131..>7266
                     /locus_tag="VHM69_08655"
     CDS             6131..>7266
                     /locus_tag="VHM69_08655"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2740502.1"
                     /translation="MKPDAREAARRVTCCVIGCGPAGAMLGLMLAREGVDVHVLEKHA
                     DFLRDFRGDTLHPSTMQIMDELGLAEGLLDLRHTKARKITARLPGRTVTVADLGRLKT
                     RFPYVTFMPQWDFLDYVTKEAKRYPTFHIEMNAEAKELILEDGIVCGVRYETLEGPRE
                     ARALLIVSADGRSSPFREQAGLKMVRTSPPIDVLWFRLSRGVGDPEDTFGYAGNGRFM
                     AVINRGDYWQIAYVIRKGEYESVRAEGLEAFQRSIGGAIPEFSNRTSELRDWDDVKLL
                     TVQADRLRRWFRPGLLCIGDAAHAMSPVGGVGINLAIADAVAAANALAGPLGAGRVRT
                     RDLMAVQLRRELPTRVIQALHSFAQRRVISSSLQQGGAPSLPGFV"
BASE COUNT         1348 a         2272 c         2352 g         1294 t
ORIGIN      
        1 cgagagcgtc ctcgggctgt ttctcgccca ggggatgccc cagaggatgc tccgctggca
       61 ctaccggagt cgccacgagt cccttataac ggtctcgaac cacgagttct acgacgaccg
      121 cctgaccatc ttcccgagcc ctgaccgtac cggagaagat ctcggactcg tctaccacca
      181 cctcgccgga accgtgtacg acaggggcaa gacccgcacc aacaccgggg aggcccgtgc
      241 ggtggcggag tcggtcatca gacacgcgaa ggagaatcca cgacgcaccc tcggcgtggt
      301 cgcgttcagt tcctcgcagg ctcaggccat cgaggatcaa ctcgagctgc tccgcagagg
      361 cgacccctca tgcgaggagt tcttctcacg gcacccgttc gagcctttct tcgtcaagag
      421 cctcgagaac gtgcagggcg acgagaggga cgtcatgttc ataagcgtcg gctatgggag
      481 ggacgccaac ggaaacctca cgatggtctt cggacccctc aatcaggacg gcggcgagcg
      541 tcggctgaac gtcctcataa cccgagcccg caggcgctgc gaggtgttta ccaacatcct
      601 gcccgacgac ctggacctcg ggtgcactga tgcgaggggc gtccggtcgc tcaagatctt
      661 tctcaggttc gcaagagaag gccacctgga agtgcccacc gcctcaggaa gggacccgga
      721 ctctcctttc gaggaggcgg ttctccgggc tttgcgggat ctcgggtacg aggtggatcc
      781 ccaggtcggg agcgccgggt tcttcgtgga cctggccatt aaagaccccg agaaaccggg
      841 acgctacctg ctcggcgtgg agtgcgacgg tgccacgtac catagcgccc gttcggcgag
      901 ggacagggac cgcctgaggc aggaagtgtt ggaaggactc ggctggcgga tacaccgtgt
      961 ctggagcacc gactggtttt ccagccccga cagcgagatg aggcgcctcg tggaggccat
     1021 tgagagagcc cgactccaca cggcccacga agacatcgcc cctgataaca ccgcgaccct
     1081 cccgcaagcc cgcgacaccg gcgaaggccc ggtcgagcgc ttcgaacggg tagagacgac
     1141 aggcgtagcc ttgccgaaat acgagacggc ccggcccaat acctcggact acaacttcga
     1201 ggtgtgggat ctacacgagg tacacctcga cgacatgtcg gcgtggatcg ccgaggttgt
     1261 ctggatcgag agtcccgtgc acctggacga catcgtcagg cgtatcgcgg aggcgtgcgg
     1321 agtgtcgcgg gcggggcgga ggatacggga gcgcgtcgag gcggggtgtc gccgggctct
     1381 gaagcaggga agggcgaggc gctccggtga cttcttctgg ctaccggaga tggatgaagc
     1441 ccctatgaga gatcggagcg ccctgcccga ctcctcgaag aagccggaga ggatagctcc
     1501 cgaggagata gggaaagccg tggagaaggt cgtgtccgag tctttcggca tgcgaaaaga
     1561 ggacattcca ccttctgtgc taaagttgct cctcggcttc agacgagcca cgaggggcgc
     1621 gtctgccacg attatggaag tcgtggagag gatgttgggg gacggcaagt tgtccgagga
     1681 gggcgatcac gtctccgttg cggacagcgt ttgaatgaag gacaaagcag cccacaaaag
     1741 atagggacag aagaacgcaa cgcccggagc ggacgcctgc agtaggtaag ctcttggtgg
     1801 tggttaggct atgtctccgc catcggtgag gatcatgacg tccgtcgcct tgacgagggc
     1861 cgtggcctgc ttgccgggtt cgaggcccag ctcgtcagcg ctctcgcggg tgatgagggc
     1921 gacaaggtgg ttgtccccga cctgtagtgt tatcttggcc gccgcttctc ctttgaccac
     1981 ctcggtaatc gtgccgggca ggcggttacg ggtgctcagt tgcatctcgc tctcctctct
     2041 ccgtggccag cagtccaagt tgtagcgcaa gcgctaccct gtggtccttc aagtctaaac
     2101 ctgtcagatt ctgtatgcgc tccaggcggt agagcatgct gttgcggtgc aggaacaggc
     2161 ggtcggcggc ctcgctcgcg ttggcgcccg ccgcgaagta cgtcgtcagg gtcctcacaa
     2221 ggtcgctacc acgcgtcctg tcttgttctc gtagcggttc gatcaggagc cggaaggact
     2281 cgagtgaaga accctcagcg agctcccgca ggagtggatc tatgtccatc gccgcctttc
     2341 cagaggtcgg aaaaggtgct gcgaccgaat ctaacagatc ccccgctcgc gtgctaaaaa
     2401 ctgtgcatga acgcctcccg ctcggatatg ttccgcaaac tgctcgaaag ggacccgaga
     2461 aaccccatgg tcctctactc gctcggcaac gagctcttca aggagggaca ctacggggag
     2521 gccagggacc atttcaagcg cgcgatcgag aacaagcctg actactccgt cgcctacagg
     2581 atgctcggcc gcgcccactt cgagcttcac gaggacaccg atgcgaggaa tatcttcatg
     2641 aagggcaagg aggtcgcaag ggaaaacggt gacctccaga ccgtcaggga gatagacgtc
     2701 ttcttgcgcc ggctggagaa gcgcgaggtg ggggagggct agccctcgat tctgctaaca
     2761 tccctggcgt gtcggaagcc aatgtaggca tcctcgtgga ctacgagagc ccgctcgccc
     2821 gcagggtggc cggtgacgtc tggtccggcc tctccagagc cgctctggat gcggggttca
     2881 cgaagacgac cgcattgtgg gagagcctgc gggagacccg gagtactatg gatctcgaag
     2941 gtccgctcgt ccacgtcttc gctatcggcc aggaccgccc cgacgccatc gacgccgtca
     3001 gcgccatagg tgaccccggt gcaccacacc tctacggcgt ggtcgtcgcc gtcccggaca
     3061 ggccgagtcc actcgcgggc tctctcctgt atcagggcgt cgagcgcctg accggtaccg
     3121 gcgtgaaggt cggcatggtc gagcccgccc tcctgcacgc cgtcccagag gagtgcgaac
     3181 gctacgccag gcacctcctc gaacggctcc ggatcccata gagacccatt ggccgcacag
     3241 cttttgaccg ccctcctcgt aaccgcggcc ttcgccggcc tcgcgtatgc gctcggcatg
     3301 atcagccaca gcggcgccct gggcgggttg ctggtcggga ccacgatcta cgtctgcctt
     3361 ggaccgcaag gcttcgcggt cctcgccctc ttcgtgatcg ggggctccct cctgacccgg
     3421 ctcggctacc gcagcaaaca acgcacaggc accgcccagg aacacggcgg acgccgcggc
     3481 gcccgcaacg ccctggcgaa ctgcgccgtc gccactctct gcgcggtcct cgctgccacc
     3541 accggctcgg aaatcttgct cgcaccgttc gtcgcctccc tcggcgcggc cttcgccgac
     3601 acgactgaat ccgagatcgg ccagctcctg agccgtactc cgcgtttgat caccaccctc
     3661 cgaaaagtcc caccgggcac cgacggtgcc gtctcgctgc caggtacact cgcaggggcc
     3721 acagcagcag gcctcactgc ggcgctcgga cttgcgctcg gcattctcga gacaccggaa
     3781 ggtgcgctcc tcgtcgcgat agcagcgttc ctgggaaccg tcgcggacag cctgatcggc
     3841 gccctctcgc cccgcgccgg caacgagttg acgaacgtac tctgcacgct cgtggcggcc
     3901 ctgcttgccc tgatcttcaa ctagaagagc gtcgaagcgc gctattcgcc gctctctaac
     3961 cgttccttca ggttccggta gcttctaggc gctcccttgg ctgccatcaa gcgaatggct
     4021 ggtacaaggg gcacgccaat cccgtgcccc ttgaagtcca gtgcgatcgt gactcgtgat
     4081 cgtgtgccgt cgtcgagagg gtcgacgatg atgttcgcgt tcggcctgat cggtccagcg
     4141 acgccctgag cggcccagct cgtgggggga ttgatctccg ttatctcctg ggtcatcgtt
     4201 cgctcagcgc gaccgatccg gcgggtcgta gtgaactttg agcccacgcc gggcggcggt
     4261 cctccctcta tgcgcacgct caagacgtcg tattgccact cggcgaacct caaggggtcg
     4321 gtaacatacg agaagacctc gtctggtggg cggtcgattt cgatgctgtg cacaattggt
     4381 ggcattgatt cctcctcgat atgtcacatc ggcagtatat ggttcgtcac tactaaaatg
     4441 acgtaacgcg acgggagaat gtgacagatg gacgatcacg actggctggc tgagcagttc
     4501 gaggagaacc gcacccatct tagggctgtg gcataccgta tgctcggctc gatcagcgag
     4561 gccgacgacg ccgtgcagga ggcatggctg cggctcagcc gctcggacac aagcggcata
     4621 gagaaccttg ggggatggct gaccagggtc gtcggcagag tgtgccttga catgctgcgc
     4681 tcgcgccgct caaggcgcga ggaacccatg ggcgcgcgtt ttcccgatcc gatcgttggc
     4741 cgcgaagacg ggatcaaccc cgagcacgaa gcgctactgt ccgactccgt aggcctcgct
     4801 ctgctcgtgg tactcgaaac tcttactccc gccgagcggc tcgcgttcgt gctgcacgac
     4861 atgttcggtg tgcccttcga ggagatagct cccatcgtgg ggcgctcccc tgttgcctca
     4921 cggaagctcg cgagccgcgc tcgccgcagg gtgcggggag cggacctggt tcccgactcg
     4981 gatctcgccc ggcagcgaga gctcgttgac gcgttcctcg ctgccgcgcg cgacggggac
     5041 ctcgacgcgc ttgtcgcggt gctcgaccct gacgtcgtgc tccgagccga caccaggggc
     5101 gtgcctctgg gcgcaccgag agaggtccgc ggcgcagcag cagtggccga gcaggcgctc
     5161 aacttctccc agcgcgctcc cttcgcacaa agagcgctgg tgaacggagc cgcgggcgtc
     5221 gtcgtggtct cgcgtgggca cccgatcgcg gtaatgggct tcgcaaccat aggcgggaag
     5281 atcgtcgaaa tcgacgtgct gtccaaccca gcccgtctcc gccagctcga ccttgcgatc
     5341 ctcgacgact gacgttcagc gccccagcgt gtcttcgccg atgccgttgc gaggtgtcag
     5401 gaaattaccc ttgacaatta tcgtacaagg atccacaata acacggtact gtttagttta
     5461 cttaaaaagg agaactcatt ggtaacggcg tctgaggagc cgagggcgag ggcgaagcgg
     5521 gaccagatcc tgactggtgc gaggcgggtg ttcctgcgcg acggcttttc tggggcgagc
     5581 acggacgcca tagcggcgga ggcgaaggtc tccaagagga ccctctacgt ctattacccg
     5641 agcaaggagg atctcttcgt ggacgtattg aggaagctaa ccatcgagaa cccccagacg
     5701 cgtgcgctgg agtccatgga gaggatgtca cctgagaaca gggaagagtt gagccgggat
     5761 ctactcgatc tggcacggaa gatcgtcgcg accatgatgc agccggacta tctcgcgttg
     5821 ctcaggacga ccatcgcgga catccacagg tttccgcaac tggcaggact cttccgcgcc
     5881 tcggtacccg agcgggccat gagcagtttc gccgtcttca tagagcggac ccgagatcga
     5941 ggggtgatcc gggacgacgt gaatggggag gcggcggctc gcatgttcgt tggtcctttg
     6001 ctcacatacg ccgtcctcga cggcctcttc gtcgccggcg gaccgccgcg aaagcccgcg
     6061 ccggggcgca tcgaacagat agtggatatg tacgtgaagg cgatcaccta gccagaaggg
     6121 aggtccaacc gtgaagcccg acgctcggga agccgcccgg cgcgtcacgt gctgtgtgat
     6181 cggctgcggc ccggcggggg cgatgctcgg gctgatgctg gcgcgcgagg gggtggacgt
     6241 gcacgtgctg gagaagcacg cggatttcct gagggacttc cgcggcgaca ccctccatcc
     6301 atcgaccatg cagataatgg acgagctggg attggcggag gggttgctcg atctcaggca
     6361 tacgaaagcg cggaagatca ctgcccgact gccaggaagg accgtcacgg tcgccgacct
     6421 cggccgcctc aagacgcgat tcccttacgt caccttcatg ccccagtggg atttcctgga
     6481 ctacgtcaca aaggaggcga aacggtaccc gaccttccac atcgagatga acgccgaggc
     6541 gaaggagttg atcctcgagg acggaatagt gtgcggggtg cgctacgaga cgctagaagg
     6601 tccccgcgag gcgcgggcgc tccttatcgt ctccgcagac gggcgctcgt ccccatttag
     6661 ggaacaggcc ggtctgaaga tggtcaggac gtctccgccc atcgacgtgc tctggttcag
     6721 gctctcgcgt ggagtgggag accccgagga caccttcggc tacgctggca acgggcgctt
     6781 catggccgtc ataaaccgcg gcgactactg gcagatcgcc tacgtcatca ggaaaggcga
     6841 gtacgagagc gttcgtgcag aaggtctcga agcgttccag cgctccatag gaggggcgat
     6901 ccccgagttc tcgaaccgta cgagcgagct gcgggactgg gatgacgtga agctgctgac
     6961 ggtgcaggcc gacagactgc gccggtggtt caggccagga ctgctgtgca tcggtgacgc
     7021 ggcccacgcc atgtcgccgg tgggtggcgt agggatcaac ctcgccatag cggatgcggt
     7081 agcggcggcg aacgcgctcg ccgggccgct cggtgcaggg cgggtgagaa cacgcgacct
     7141 gatggccgtg cagctcaggc gggaactgcc gacgcgcgtg atacaggcac tacactcctt
     7201 cgcccagcgg cgtgtgatct cctcgtccct gcaacagggt ggagcgccgt ctctccctgg
     7261 attcgt
//
LOCUS       DATAIR010000355         7494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_392019, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000355 DATAIR010000000
VERSION     DATAIR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392019"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VHM69_08660"
     CDS             complement(<1..1263)
                     /locus_tag="VHM69_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX2740503.1"
                     /translation="MSSTMDQAREIARGPFEPTWESLRQFECPGWFRDAKFGIWSHWG
                     PQSVPMYGDWYARHMYLEGHDQYRHHWRVYGHPSKVGYKDLVPLWKAERFDPEDLMRR
                     FVRAGAKYFVGQAVHHDNFDNFDSSHNRWNAVNVGPEKDIVGLWREAAGAHDLPFGLT
                     EHLGASFNWFGVNKGSDASGPYAGIPYDGNDPEFEDLYYRNRGYDLTKDGQRTWYTDN
                     EAFHAHWFARIKDVIDRYRPDLLYSDGGVPFGETGRRIVAHLYNTSAATHGENRAVYA
                     QKDPDPDVYTVGVLDIERGQRDEILEHPWQTDTSVGDWFYNVRDVYKTPRHVLEMLVD
                     IVSKNGNLLLNIPQRPDGTLDDECLHLLDRMEAWMRPNGEGIFGSRPWEISGEGPSSA
                     EGGAFKEAAIAWTAEDFRFTSREETLYAY"
     gene            complement(1428..2309)
                     /locus_tag="VHM69_08665"
     CDS             complement(1428..2309)
                     /locus_tag="VHM69_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2740504.1"
                     /translation="MSLLDSIGAALKGKGSEAPGSKAHWPILIYATLMAMAFLTLVPI
                     VWMVLTAFKSAPEVAANPPTWWPREWHPENFAAAWNFAPFGRYLINSLIMVGGITILQ
                     TVTSALAAYAFARLQFRGRDLLFLLYLGTLMIPPQVTVIPQFILIKELDWVDTYQGLI
                     IPQAFTAFGVFLLRQFFLGIPRELEDAARIDGASRFGCFWRIILPLSGPALATLAVFA
                     FLFHWNNLLWPLIVSNSDATVPVVVGLRNFQGQYGTEWNLLMAAAAIATIPTVIVYLF
                     AQRWFVRGITMSGFGGR"
     gene            complement(2322..3245)
                     /locus_tag="VHM69_08670"
     CDS             complement(2322..3245)
                     /locus_tag="VHM69_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2740505.1"
                     /translation="MTSTARTGRTSEKVPWTKREHEGLAAGLFLLPNLIGFLIFTAIP
                     VGAAFVLAFYDYDLLLGASWAGLENFREMFTTDEVFRAALFNTVYFTAVSVPLSVVLG
                     LATAMLVNQALRGIVIFRSIFLLPYVTVTVALSLVWKWIYLPDIGLINSVLGVFGIDG
                     PPWLTSGTWAMPALIIMSVWKGFGYNMVIFLAGLQGIPDHLYDAAKVDGATAWRRFLN
                     VTLPLLSPTTFFVVVISVISSFQVFDQALIMTNGGPGTATTTLVLYIYQKGFQSFDMG
                     YAAAVALVLFAAIFVFTVIQFLFQRRWVTYD"
     gene            complement(3254..4624)
                     /locus_tag="VHM69_08675"
     CDS             complement(3254..4624)
                     /locus_tag="VHM69_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2740506.1"
                     /translation="MVSGRRARGGRTERLTAALAGRAMNRRDFLRMSGAGLAGVGLLG
                     AGACGGGGGTSGQLRWSMWSDTPEETKVWKGLAEDVHKKNPDITVKLETVTFADYWDK
                     LSTQIASENEADIVAMQSLRMPGFAVRGALQPLQPFIEDDSGFDVEDFFEPIRDGLSS
                     EGEIYALGYDIGPILLYYNKDLFNKAGVEPPSPETPMSWEEFREKAEQLTDPAAKEYG
                     FVTQPVFDSIVPWLWSGGGDYMNAAETECTLDTPESIEAMEFVISMIDDGIAAPVTDL
                     ANTSFAIEEFFSGKVGMHIDGPWQFINIKENSDFEWDVAPLPAGPAGSVTPAAGSGFG
                     ISNTTENPDAAWEALKILTSTGSLEKLVKAGRGYPARESAVPTFENPPPANVDVVQKI
                     LVSDIGEARPFVTTTTWQETTVMMGRDFSPVFLGKQSVEEVVAKVKPQFDELLQEHQE
                     ILEKQS"
     gene            4822..5514
                     /locus_tag="VHM69_08680"
     CDS             4822..5514
                     /locus_tag="VHM69_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2740507.1"
                     /translation="MTLPVDAANIGSRNVGVRGDQVKTVLCYGDSNTWGSDPETGERF
                     PEDVRWPGVLARNLGDGYRVIEEGLPGRTTVWDDPIEGDYKNGRNYLTPCLESHRPID
                     LITLMLGTNDLKWRFGTSASDIAQAASSLAEIMMRSGCGPEGGAPVVVLISPPPVAKL
                     TDMAQMFDGAGEKSGQFPEHYARFAEKIGCEFMDASQVIVSSDVDGIHLDAGEHRKLG
                     EAVAGRIRQILE"
     gene            5511..6662
                     /locus_tag="VHM69_08685"
     CDS             5511..6662
                     /locus_tag="VHM69_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2740508.1"
                     /translation="MRILLVGGPMYDPLYARIAEFAEREGVKVETVIAPTHPDLNERI
                     EEEFRAGDARYDLISTHTKYAPSQKQWLTPLDEDLEDWELEHFTPRTLELARIEGHLY
                     GVPRNLDVKLLMYRTDLMEDQPSSWEDLLETAARLRSTELYGFVFPGKESGLFGHFFE
                     LHAMAGGRMFEDNGPPAPHLDDEAGLWALGLLKNLYDRAAPPETPDWHYDEVAACFRE
                     GKAAMSTDWPGGFYTYVDPERSRILDRFDVALYPEGSAGRHIYSSSHTFAIPTTVSDR
                     PGAVELLRFLTSRESQAYEARLGTLPARSDSLHDARSEAEAGSLAERRWRLLERAQEA
                     ALLPPKHPNYPAVEDAIWEGIREALLGNKSVEKALEDTEAAARRAAEGE"
     gene            6686..>7494
                     /locus_tag="VHM69_08690"
     CDS             6686..>7494
                     /locus_tag="VHM69_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740509.1"
                     /translation="MAESRVVDPASRRTLGSTGLSVSPVCIGCAPLGDMPETFDFGVG
                     EEQARETLWAVFESPINFLDTAAAYGDGESERRIGEVLKETGGLPDGVVLATKADRDL
                     TTGDFSGEQMRRSVERSLRLLGLDKLQLVYLHDPEYLNETGRDPFEYMMSDGGPVEVL
                     REFEEEGVIEHLGIAGGPVELLIRFVETNLFEAVITHNRYTLVNRTADPLFDVAAEHG
                     VAALNAAPYGSGILAKGPDAYARYEYQEAPKEMIEQVRAMQDACSEFDVPLA"
BASE COUNT         1415 a         2330 c         2369 g         1380 t
ORIGIN      
        1 gtaggcgtag agcgtctcct ccctgctcgt aaagcggaag tcctcagccg tccacgcgat
       61 cgcggcctcc ttgaaggctc ccccttctgc gctggacggt ccttcgccgg agatctccca
      121 ggggcggctg ccgaagatgc cttctccgtt gggtcgcatc cacgcctcca tgcggtcaag
      181 cagatggagg cattcatcgt ccagggtgcc gtcgggtcgc tgggggatgt tcagcagcag
      241 gttgccgttc ttgctgacga tgtccaccag catctcgaga acgtgccgcg gggtcttgta
      301 gacgtcgcgc acgttgtaga accagtcacc gacgctggtg tcggtctgcc aggggtgttc
      361 gaggatctcg tcgcgctgcc cccgctcgat gtcgagcacg cctacggtgt atacgtcggg
      421 gtccgggtcc ttctgggcgt agaccgcccg gttttcgccg tgtgttgccg cgctcgtgtt
      481 atataggtgg gctacgatgc gccggccagt ctcgccgaac ggcacgcccc cgtccgagta
      541 gagcaggtca ggccggtagc ggtctatcac gtccttgatc cgcgcgaacc agtgcgcatg
      601 aaacgcctcg ttgtctgtgt accacgtccg ctgcccgtcc ttcgtgaggt cgtagccccg
      661 gttgcggtag tagagatcct cgaactcagg gtcgttgccg tcgtagggga taccggcgta
      721 ggggccggac gcgtcgctcc ccttgtttac accgaaccag ttaaagctcg cgcccaggtg
      781 ctccgtcaac ccgaagggca gatcgtgcgc cccggcggcc tctcgccaca ggccgacgat
      841 gtccttctcc ggaccgacgt tcaccgcgtt ccagcggttg tggctggagt cgaagttgtc
      901 gaagttgtcg tggtgcacgg cctggccgac gaagtatttg gccccggccc ggacgaagcg
      961 acgcatcagg tcttcggggt cgaacctctc cgccttccac aacggcacga ggtccttgta
     1021 gccgaccttc gaagggtggc cgtagacacg ccagtggtga cgatactggt cgtgcccttc
     1081 gaggtacatg tggcgggcgt accagtcgcc gtacatcggc acggattgcg gcccccaatg
     1141 ggaccagatg ccgaacttcg cgtcgcgaaa ccaccccgga cattcaaact gccgcaagga
     1201 ctcccaagtg ggctcaaagg gtcctcttgc gatctccctg gcctggtcca ttgtcgagct
     1261 cacccctctt tcttcaccgt ttttcctccc tgtcgggcgg catcaccaaa ggctggacca
     1321 ggaggggctc cgggtgtttt gaaccatctg gtgtcggcgg tcgcggacct ccactgaagc
     1381 tcctgtgtca aggctcctcc ttgtctgcga tgagatctgc agcgtctcta cctgccaccg
     1441 aatccggaca tggttatgcc cctgacgaac caacgctggg cgaagaggta gacgatgacg
     1501 gtcgggatgg tggcgatagc ggcggcggcc atgagcaggt tccattctgt cccgtactgt
     1561 ccctggaagt tcctcaggcc gaccacgacg gggaccgtcg catcggagtt cgagactatc
     1621 agcggccaga gaagattgtt ccagtggaag aggaaggcga agacggcgag cgtcgcgaga
     1681 gctggtcccg agagcggaag gatgatgcgc cagaagcaac cgaagcggct cgcgccgtcg
     1741 atgcgtgccg cgtcctccaa ctcgcgcggg atgccgagga agaactgacg caagaggaag
     1801 accccgaacg ccgtgaacgc ctgggggatt atgaggccct ggtaagtgtc cacccagtcg
     1861 agttctttta tcaggatgaa ctgcggtatg acggtgacct ggggtgggat catgagtgtc
     1921 cccagataga gtaagaagag caggtcgcgt cccctgaact gtagacgggc gaaggcgtag
     1981 gctgccagtg ccgaggtcac cgtctggagg atggtgatgc ctccgaccat gatcaggctg
     2041 tttatcaggt acctgccgaa cggggcgaag ttccaggccg ccgcgaagtt ctcgggatgc
     2101 cactccctcg gccaccacgt cggcgggttg gccgcgacct cgggggccga cttgaacgcc
     2161 gtcaggacca tccagacgat cgggacgagc gtcaggaacg ccatcgccat cagcgtggcg
     2221 tagatcagga tcggccagtg cgccttgctg ccgggtgctt cactcccctt ccctttcagg
     2281 gccgcgccga tggagtcgag taggctcatg cgttcttacc cctagtcgta tgtgacccag
     2341 cgtcgctgga acaggaactg gataacggtg aagacgaata tcgcggcgaa caggaccagg
     2401 gcgaccgcgg cggcgtagcc catgtcgaag gactggaagc ccttctggta gatgtagagg
     2461 accagggtcg tcgtcgcggt tcctggtcct ccgttcgtca ttatcagggc ctggtcgaag
     2521 acctggaaag aggagatcac ggagatcaca acgacgaaga acgtcgtcgg ggagagcagg
     2581 ggcagggtga cgttcaagaa cctgcgccat gccgtcgcgc cgtcgacctt cgccgcgtcg
     2641 tagaggtgat ccggtatccc ctgcaagccg gccaggaaga tcaccatgtt atacccgaac
     2701 cccttccaca cactcattat tatcagggca ggcatggccc aggtcccgct cgtgagccac
     2761 ggcggtccat ctatcccgaa cacgccgagc acggagttga tcaggcctat gtcgggaagg
     2821 tagatccact tccacaccag cgagagcgcc accgtgaccg tcacataagg aagcaggaag
     2881 attgaacgga agatgacgat gccgcgcagc gcctggttga cgagcatcgc ggtcgcgagc
     2941 ccgagcacga ccgagagcgg cacgctgaca gccgtgaagt aaacggtgtt gaagagcgcg
     3001 gcccggaaga cctcatccgt ggtgaacatc tcgcggaagt tctccaggcc cgcccagctc
     3061 gccccgagga gcagatcgta atcgtagaac gcaagaacga aagcagcccc tacaggtatg
     3121 gccgtaaaga tgaggaaccc gatcaggttc ggcagcagga agaggccggc ggcgagcccc
     3181 tcatgctcgc gctttgtcca gggaactttc tccgacgtcc gcccggttcg cgccgtgcta
     3241 gtcacggaac cccctacgac tgcttctcga ggatctcctg gtgctcctgg aggagctcgt
     3301 cgaactgcgg cttgaccttg gcgacaacct cctcgacgct ctgcttgccg aggaacacag
     3361 ggctgaagtc gcgtcccatc atgaccgtcg tctcctgcca ggtcgtcgtc gtcacgaagg
     3421 gcctggcctc accgatgtcg ctcacgagta tcttctggac gacgtctacg ttagcgggcg
     3481 gcgggttctc gaaggtcggt accgctgact cgcgggcggg gtaaccgcgt ccggccttga
     3541 ccagcttctc cagagaccct gtgctggtca ggatcttgag tgcctcccac gccgcatccg
     3601 ggttctcggt ggtgtttgag atgccgaagc cggagccggc ggccggagtt acgctacccg
     3661 ccggtccggc aggcaacggc gccacgtccc actcgaagtc ggagttttct tttatgttga
     3721 tgaactgcca gggaccgtca atgtgcatcc cgaccttgcc gctgaagaac tcctcgatcg
     3781 cgaagctcgt gttcgcgagg tcggtgacag gcgcggctat cccgtcatcg atcatgctga
     3841 tcacgaactc catggcctcg atggactcgg gggtgtcgag cgtgcactcg gtctcggctg
     3901 cgttcatata gtctcctccg cccgaccaca gccacgggac tatggagtcg aagacgggct
     3961 gcgtgacgaa cccgtactcc ttggcggcag ggtcggtgag ctgctccgcc ttttccctga
     4021 actcttccca gctcatcggt gtctcaggtg atggcggctc cacgcccgct ttgttgaaga
     4081 gatccttgtt gtagtagagg aggatcgggc ctatatcgta accgagtgcg tagatctcac
     4141 cctccgacga taatccatcg cggattggct cgaagaagtc ctcgacatcg aacccggaat
     4201 catcctcgat gaagggctgt aacggctgca gggcgccacg cacggcgaat ccgggcatcc
     4261 tgaggctctg catggcgacg atgtcggcct cgttctcgct tgcgatctgc gtggagagct
     4321 tgtcccagta gtcagcgaat gtgacggtct ccaacttcac cgtgatgtcc gggttcttct
     4381 tgtgcacgtc ttcggcgaga cccttccata ccttggtctc ctccggagtg tcagaccaca
     4441 tcgaccaccg caactgcccc gaggtgccgc caccaccgcc gcaagccccg gcacccagca
     4501 aaccgacacc cgcgagacct gcgccgctca tcctcaggaa gtccctgcgg ttcatcgccc
     4561 tccccgcgag ggccgcggtt aaccgctccg ttcgtccacc acgcgcccga cgaccggaaa
     4621 ccatcgctgc tctcccttct gcccgcttca cgcggccaca atcgcttccc ccatcaccct
     4681 ctaacggaca ttacccgagg agcttgcccg cgtcaagaaa taagtaggac ctttcctatc
     4741 gtgagaggtt gtcaatcggg atattgggct gattagggca ctaaacatct gtcgtttgtc
     4801 tgtactccgg gcgctctgcg gttgactctg ccggtggatg ccgccaacat tggcagcagg
     4861 aacgtcgggg tgagagggga tcaggtgaag acggtacttt gttacgggga ctcgaacacg
     4921 tggggctcgg accccgagac gggcgagcgg tttcccgagg acgtgcgctg gcctggggtg
     4981 ttggcccgta atctgggcga tgggtatcga gtgatcgagg agggtctgcc agggaggacg
     5041 acggtctggg acgatcccat agagggagat tacaagaacg gcaggaatta tctgacgccg
     5101 tgcctggaat cgcaccgacc catagacctc atcacgctca tgctggggac caacgacttg
     5161 aaatggcgct tcggcacctc ggcctcggac atcgcgcagg cggcctccag cctggcagag
     5221 atcatgatga ggagcggatg cgggcccgag ggaggcgctc ccgtcgtagt tttgatctcc
     5281 ccaccgcctg ttgcgaagct cacggacatg gcgcagatgt ttgatggagc cggggagaaa
     5341 tccgggcagt tccccgagca ctacgcgcgt ttcgccgaga agatcgggtg cgagttcatg
     5401 gatgcctctc aggtaatcgt ctccagcgac gtggacggca tccacctcga cgcgggcgag
     5461 caccgcaagc tcggcgaggc cgtcgccgga cgcataaggc agatactcga atgagaatcc
     5521 tgctggtcgg tggaccgatg tacgatcccc tgtacgcgcg catcgccgag tttgcggaac
     5581 gcgaaggcgt caaggtcgag actgtcatcg caccgacgca tcccgacctc aacgagcgga
     5641 tcgaggagga gttccgtgcg ggcgatgcta ggtacgacct gatctcgacg cacaccaaat
     5701 acgccccgag ccagaagcaa tggctcaccc cattagacga agatctggaa gattgggagt
     5761 tggagcactt cacccctcga accctggagc ttgcgcgcat agaaggccat ctctacggcg
     5821 tcccgcgcaa ccttgacgtg aagctgctca tgtaccgcac ggacctgatg gaagatcagc
     5881 cctcctcctg ggaggacctg ctcgagaccg cggcgcgtct ccggtctacg gaactttacg
     5941 gcttcgtctt ccctggaaag gagtcggggc tgttcggcca cttcttcgag ctgcacgcga
     6001 tggcaggcgg caggatgttc gaggacaacg gcccgcccgc gccgcacctc gacgacgagg
     6061 ccggactatg ggcgctcggt ttgctcaaaa acctctacga tagggccgcg cctcccgaga
     6121 cccctgactg gcactacgat gaggtcgccg catgtttcag ggagggcaag gccgcgatga
     6181 gcacggactg gccaggcggc ttctacacct acgtcgatcc ggagcggagc aggatcctgg
     6241 accgcttcga cgtggcgctt taccctgagg gatcagccgg ccgccacata tactcgagct
     6301 cgcacacctt cgccatacca accaccgtca gcgaccgtcc gggcgccgtc gagctgttgc
     6361 gcttcctgac ctcgcgcgag tcgcaggctt atgaggccag gctggggaca cttcccgcga
     6421 ggagcgactc cttgcatgac gcgcgctccg aggccgaagc aggttcgctg gcggagcggc
     6481 gttggaggct gctggagagg gcgcaggaag cggcgctcct cccgcccaag catccgaact
     6541 atccggcggt tgaggacgcc atctgggagg gcatcaggga ggcgctcctt gggaacaaga
     6601 gcgtggagaa agcgctcgaa gacaccgagg cagcggcgcg gcgcgctgcg gagggcgagt
     6661 aaaacctcga accgggagga tccggatggc tgagagcaga gtcgttgacc ccgcttcgag
     6721 gaggaccctg ggcagcacgg gcctctccgt ctcgcctgtg tgcatcgggt gcgcgcctct
     6781 gggagacatg cccgagacct tcgacttcgg ggtgggcgag gagcaggcgc gagagacgct
     6841 gtgggccgtc ttcgagagcc cgatcaactt cctcgatacc gcagctgcct acggcgacgg
     6901 ggagagcgag cgccgcatcg gtgaggtatt gaaggagacg ggcggactac cggatggcgt
     6961 cgtactcgcc accaaggccg accgggacct cacgaccgga gacttctccg gcgagcagat
     7021 gcggcgctcc gtcgagcgct ccttgcggct actcggactc gacaaactcc agctcgtgta
     7081 cctgcacgac cccgagtacc tgaacgagac gggccgtgat cccttcgaat acatgatgtc
     7141 tgacggaggt ccggtcgaag tgcttcgaga gttcgaggaa gaaggcgtca tcgaacacct
     7201 cggcatcgcg ggaggacccg tggaactcct gatccgattc gtcgagacaa acctttttga
     7261 ggccgtgatc acccacaacc gctacacgct cgtcaacaga accgcagacc ctctcttcga
     7321 cgttgccgca gagcacggag ttgccgccct gaacgcagcc ccctacggca gcggcatcct
     7381 ggccaagggc cccgacgcct acgcccgcta cgagtaccag gaagccccga aggagatgat
     7441 cgaacaggtt cgcgccatgc aggatgcctg cagcgagttc gacgttcctt tggc
//
LOCUS       DATAIR010000356         6336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_115638, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000356 DATAIR010000000
VERSION     DATAIR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115638"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..772)
                     /locus_tag="VHM69_08695"
     CDS             complement(41..772)
                     /locus_tag="VHM69_08695"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX2740510.1"
                     /translation="MDRFWEVDATRGVAIIMMAVYHTAYDLDVLGGYDIQSTSGNWAL
                     FADLTAGLFLFLVGVSLAISRARTGRTGWSLFRKYLARGLRILAYGMVLTVVFLVLRM
                     GVVAFGILHLIGVSIILAYPFLKLRFTNLVLGVLIFAVGWYIMAQDPTSESFLLLPFG
                     VVPEGVIMPDYRPLLPWFGVVLIGLFFGNVVYGRGRRPATDKAPLPLLPLLPLGRNSL
                     FIYLIHQPVIIALLAAFGLVNLNFM"
     gene            878..1357
                     /locus_tag="VHM69_08700"
     CDS             878..1357
                     /locus_tag="VHM69_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740511.1"
                     /translation="MSTDFTIRPGRREDAAEAARLWMQSAEEHKTHDRVYATAPGAEK
                     TMRRFLADVANSGFSFLFVAVAGERTVGFISGELRQGSPTFLPKTWASVDDVFVEPDH
                     RNLGVGRALLQSVEAWAKKRNADGISLQVAAANSRGRKFYEELGFREISVYEVLEFE"
     gene            complement(1361..1834)
                     /locus_tag="VHM69_08705"
     CDS             complement(1361..1834)
                     /locus_tag="VHM69_08705"
                     /inference="COORDINATES: protein motif:HMM:NF023982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740512.1"
                     /translation="MKPIEVAPVPATGLDAPLALLEESLRDGEPVPKRFSDRLRKAVE
                     AGDIEILAARTEDRMLGVIVLAYRLNVSVGGLFASIEDLYVRPEARRRGVGRALLEAA
                     DERFAACGVSYVEVQVEDDEAEAFYSALGYERETNVRVLSRSLPIGANEDLIPEA"
     gene            complement(2241..3173)
                     /locus_tag="VHM69_08710"
     CDS             complement(2241..3173)
                     /locus_tag="VHM69_08710"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX2740513.1"
                     /translation="MATKIALCIGQNEYDPSTRVTPLRGCVNDTLLIGEMLRRAGFEV
                     RQIHNQAATQRGILDRLSTEVAKLREGDYFVFWNSSHGYQLNDREGDELYDYLDEAIC
                     TYDTDPRDPLTDDKLGRIVYRADPKATVFLGSDSCHSGSLTRAMLEDQTGNGGPKPRL
                     WIPPDDVRFRSGTTLIDLDAFAEGSEKSLVDHKASGKEAVEQIQPTKQELRRFGHVGR
                     EIEEKEMGHLLLSGCRPEQTSADAPFPEGWYGAMTYNFAKAVLRAWRSGNAITYGEAH
                     KAALAGLEQGEFEQKPQLEGPDQLKDTPVFGFKP"
     gene            complement(3548..4189)
                     /locus_tag="VHM69_08715"
     CDS             complement(3548..4189)
                     /locus_tag="VHM69_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740514.1"
                     /translation="MDTSQREYQRLEDPPVDGTDEPEAEKVPGQINFDPDEIEVATEE
                     DIQRIIQEEGKVSEPEIGAFELGAPAPSSGGMAASRSYISDHPTHFDFRADVARAINR
                     VQAKFPSQTYANTYYMHPPVYGRKYEFVSVDFWGGGLSNGRYAGYRGKPIGTSLGNQV
                     WKAIYNDPSLPNIFWIIWNGKMWVRGTGWESAPWGPPDSDAGHYKHIHVTYVL"
     gene            complement(4418..5455)
                     /gene="ltaE"
                     /locus_tag="VHM69_08720"
     CDS             complement(4418..5455)
                     /gene="ltaE"
                     /locus_tag="VHM69_08720"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX2740515.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMLEAPVGDDVFGEDPTVKRLEEYVA
                     DLLGKEAALYAPSGTMTNQIGVHVNTNRGEEVLLHEGSHIFVYEAGAPAMLSSVQVRT
                     LPGENGVLAPDTVRAAVRTDDVHFPRSRLVCLENTHNTAGGKVFPLEDFAAVAATARD
                     LRLKVHLDGARLFNAQAATGTPAREWCAHADTVSVCSSKGLGAPVGSLLAADEETISE
                     ARRARKAFGGGMRQAGIIAAASLYAFENNIDRLAEDHERARTLASGLREAGYEAQEPE
                     TNLVLVAVDKPPEFLDALAREGILATPGKPGFIRLCTHLDVRGVLGTEEGIQQVVQSF
                     LRASENTRQPV"
     gene            complement(5452..>6336)
                     /locus_tag="VHM69_08725"
     CDS             complement(5452..>6336)
                     /locus_tag="VHM69_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740516.1"
                     /translation="GIEIAGSRPIDHGTQYDLTRAGETAKLNVYHTGKISTGGRASGL
                     RDLLEGWRTSQTKGENRRVFTPPAGGRPVLDGTPRLGIDESGKGDYFGPLVVAGVRIT
                     GEEEAKSLREIGVRDSKTVSVLGIRRIADRILETVGEDNVCVRSLPPREYETRRRSAG
                     NVNRLLGELDAEIIGELANEVDLVVVDEFARSARSHLEPFVPEGVRLEVRTRAEDDAA
                     VAAASIVARARQLDEMDRLSERVGFKLPLGATHVVRAGRRIVEELGEEGLDEVAKTHF
                     ATTRRVLQLVQHRDGGNP"
BASE COUNT         1217 a         1942 c         1835 g         1342 t
ORIGIN      
        1 cctgctccgc ggcgaaaaag aaacatccca gcctgaagtt ctacataaag ttcaggttca
       61 cgaggccgaa tgcggcgagc aaggcgatga ttacgggctg atgtatcagg tagataaaca
      121 acgagttcct accgagcgga aggagtggaa ggagcggcag cggagccttg tccgtagcgg
      181 gtcgccggcc tctaccgtac accacgttcc cgaagaacag cccgatcagg acgacaccga
      241 accagggcaa cagcggcctg taatcgggca tgatcacacc ctcggggacg acgccgaagg
      301 gcagcagcaa gaagctctct gaggtgggat cctgggccat tatgtaccaa ccgaccgcga
      361 atattagtac gccgagcacg aggttggtga aacggagttt caagaaggga taagcgagga
      421 ttatggagac cccgatcagg tgcagtatcc cgaaagccac gacgcccata cggagtacga
      481 ggaagacgac ggtcaggacc atcccgtaag cgaggatcct caatcctctc gccaggtact
      541 tgcggaagag actccacccc gtccgcccgg tcctcgcgcg gcttattgcg agcgagacgc
      601 caacgaggaa cagaaagagg ccggcggtaa ggtcggcgaa gagcgcccag ttcccggaag
      661 tagattggat gtcgtagcct ccgaggacgt ccagatcgta ggcggtgtgg tagaccgcca
      721 tcattattat ggcgacgccg cgggttgcat ccacctccca gaacctgtcc actttctgtc
      781 ccgcgctccg ctccgtactc ggcctctctc cggtttttcc ggatcatacc ccacccccac
      841 ccgtagcgga ccgcggctca tgggttagga ttgcggggtg agcacggact tcaccatccg
      901 gccgggacgc agggaggacg ctgccgaggc ggcgcgcctg tggatgcaga gcgccgagga
      961 acacaagacc cacgaccggg tctacgccac cgcgccaggc gcggagaaga ccatgcgacg
     1021 ctttctggcc gacgtggcta atagcggctt ctctttcctc ttcgtggccg tagccgggga
     1081 gagaacggtg ggctttatct cgggtgagct gcgccagggc tccccgacct tcctcccgaa
     1141 gacgtgggcc tccgtggacg acgtgttcgt cgaaccagac catcgcaacc tcggcgtggg
     1201 ccgcgccctc cttcagagcg tcgaggcgtg ggcgaagaag aggaacgcag atgggatctc
     1261 tctccaggtc gccgccgcca actcgcgggg ccgcaagttc tacgaagaac tcggcttccg
     1321 tgagatctcc gtctacgaag tgcttgagtt tgaataggta tcaggcttca ggtatcaggt
     1381 cctcgttcgc gccgatgggt agggatcgag agaggacccg gacattggtc tcccgctcgt
     1441 agccgagcgc agagtagaac gcctcggcct cgtcgtcttc gacctgtact tcaacgtaag
     1501 agacgccaca cgctgcgaaa cgttcatctg ctgcttcaag cagggcgcgg ccaacgcccc
     1561 ggcgtctggc ttctgggcgc acgtagaggt cttcgatgct ggcgaagaga ccgccgacgg
     1621 agacgttgag gcggtatgcg aggacgatga cgcccagcat ccggtcttcc gtccgtgcgg
     1681 ccagtatctc gatatctccg gcttcgacgg ccttgcgaag gcggtcggag aaacgtttgg
     1741 gtacgggctc gccgtccctg agagattctt ctagcagcgc cagcggcgcg tcgaggccgg
     1801 ttgcggggac aggggcgacc tcgatcggct tcaaagctga aggctgatgg tcgccgctgt
     1861 caaggtgggt acaacagtcc gagaaccgat aacctcgcgt atctccacgt cccccctgtc
     1921 tactagcccg tctagctgca cctctagatc ttccagcatt tgcaggtctc ttgccgccct
     1981 ggtcagagat tccaaccgtt gccgtgccac atcctccagt acagcgtgag ctgcctcaat
     2041 ctcggcttac ggtgatctct ccttgtcaat cagggtgtga atttgcgtct tcgagtcttg
     2101 cgggtgcacg ttacggcgag cccaatatac ctcgtgttac aggttatccg cacgcatcca
     2161 cctaccgtgc cggccgcgaa cgtcttacga gtaactgtgg tcgttcctgt ggatgacccg
     2221 tgccaccagt ccaatgctaa ctagggcttg aacccgaata ccggagtgtc tttcaattga
     2281 tctggccctt ccagctgtgg cttctgttca aattcgcctt gctccaggcc tgcgagggca
     2341 gccttgtgcg cctcgccgta ggtgattgca ttaccgctcc tccaagctct caacaccgcc
     2401 ttggcaaagt tgtaagtcat agctccatac caaccctctg ggaacggagc atcggcactt
     2461 gtctgttcgg ggcgacagcc acttaggagc aggtgaccca tttccttttc ttcgatctct
     2521 cttcctacat ggccaaagcg ccttaattcc tgtttggtag gttggatctg ctccaccgct
     2581 tctttgccag aagctttgtg gtctacgaga gacttttcac taccttcagc aaaagcgtcc
     2641 agatctatga gggtggttcc actgcgaaag cgtacgtcat ccggaggtat ccatagtctc
     2701 ggctttggac caccatttcc tgtctgatct tcgagcatcg cgcgcgtcaa ggagccgctg
     2761 tgacaagagt cagagcccaa gaacacggtt gccttcggat cagctctgta gacgattctg
     2821 ccaagcttgt cgtctgtgag ggggtcccgg gggtccgtgt cgtaagtgca tatcgcctca
     2881 tccagataat cgtagagctc atcaccctct cggtcgttca gttgatagcc atgggatgaa
     2941 ttccagaaga cgaagtagtc tccctctcgg agcttggcca cctcggtact tagccggtcc
     3001 aagatccccc gttgtgttgc cgcctgattg tgaatctgac gtacctcgaa gcctgcccta
     3061 cggagcatct cgccgatgag cagcgtatcg ttgacgcagc cgcgcagtgg tgtaactctg
     3121 gtagaggggt catactcgtt ttgtcctata catagagcga tctttgttgc caaggtcttt
     3181 ctcctctaat tcggcctgca caaggcttat tcacctgggt gtctagagca actatccaca
     3241 gagcctcgat ggtctgtctt gcgggctcac gaatcgagca agggaggcca gtttccccgt
     3301 gacaacgaaa gagtaccgtt gagctacctt ttggacggta tctacagcaa tttgcagaaa
     3361 aactgatcct tcgaaactgt atccctgccg gaaaccaaac cccaagcatc tgaggctccg
     3421 cgaaacggtg gatcagttgt ttcgagaaac gctctagaag tggtgctctc ggaagttcat
     3481 ccattcctag ttacagaagg tcctgtaata cgcctcctta cctcctcacg ttaactacga
     3541 ctgcaaacta caacacgtag gtgacgtgta tgtgcttgta gtggcccgcg tcggaatccg
     3601 gcggacccca cggcgcggac tcccaccccg tcccgcgcac ccacatcttg ccgttccaga
     3661 tgatccagaa gatgttcggc aaggacggat cgttgtagat tgccttccat acctgatttc
     3721 caagggaggt tcctatcggc ttgccccggt aaccggcgta tcgaccgttc gagaggccac
     3781 cgccccagaa gtccacgctg acaaactcgt acttcctgcc gtagaccgga gggtgcatgt
     3841 agtaggtgtt cgcgtaggtc tgcgacggaa actttgcctg cacccgattt atggcgcgtg
     3901 ccacgtccgc gcgaaagtcg aagtgcgttg ggtgatccga gatgtacgat ctgctggccg
     3961 ccattccgcc ggatgatggt gccggggctc caagctcgaa ggctccgatc tcgggctcgc
     4021 tcaccttacc ctcttcttgg atgattcgtt ggatgtcttc ttccgtagcg acctctatct
     4081 cgtccggatc aaagttgatc tgcccgggaa ctttttccgc ctcgggctcg tcggtcccat
     4141 cgaccggagg atcctccagc ctctgatact ccctctggct agtgtccaag atcttctcct
     4201 catgctcgtg tggtcttctt ggccaaacta gcaccctgca atgacgccgc gatgaatcga
     4261 cgatgaattt gtaatgaata tgctttttgc acgatttttt ctctcggacg agcgtgtgca
     4321 ctgcgatgtc ggccctgctg cttagggata acacacaaca gagcccgccc gtactcgtga
     4381 gaagagacgg gctcaaggtt agacgagcta ggttagctca taccggctgg cgtgtattct
     4441 ccgaagcacg aaggaaactc tggacgacct gctgtatccc ctcttcggtt cctaaaacgc
     4501 cacgcacgtc gaggtgggtg cacaagcgaa tgaagccggg tttgccagga gtggcgagta
     4561 tgccttcacg ggcgagcgcg tccaggaact cgggaggttt gtcgaccgcg acgaggacga
     4621 ggttggtctc gggttcctgg gcctcgtatc cggcttctcg caggccggag gcgagagtac
     4681 gggctcgttc gtggtcctcg gcgagcctgt ctatgttgtt ctcgaaggcg tagagtgagg
     4741 ctgcggcgat gatgcctgcc tgccgcatcc cgcccccgaa cgccttgcgg gcacgcctcg
     4801 cctcgctgat ggtctcctcg tccgcggcga gaagggaccc gacaggtgct cccagtccct
     4861 tggaagagca tacggagacg gtgtcggcgt gcgcgcacca ctcgcgggca ggggtacctg
     4921 tcgcggcttg cgcgttaaag agtctggcgc cgtcgaggtg gactttgagg cggagatccc
     4981 tggccgttgc ggcgaccgca gcgaagtcct ccagcgggaa gaccttgccg ccggcggtgt
     5041 tgtgcgtgtt ttccagacag acgagcctcg acctcgggaa gtggacatca tctgtcctga
     5101 cggcggcgcg cactgtatct ggtgcgagga caccattctc accagggagg gtgcggacct
     5161 gtacgctgga gagcatagcg ggtgcgccgg cttcgtagac gaagatatgg gaaccctcgt
     5221 gcagaagcac ctcctcaccc cggttcgtgt tgacgtggac cccgatctgg ttggtcatcg
     5281 ttccggacgg cgcgtacaga gccgcctcct tgccgagcaa gtcggccacg tactcctcga
     5341 gccgctttac cgtcgggtcc tccccgaaaa cgtcgtcccc gaccggcgcc tcgagcatgg
     5401 cccgccgcat gccctccgtc ggacgggtca cagtgtcgct gcgaagatct atcaagggtt
     5461 acctccatct ctgtgctgta ccaattggag cacccgacgc gtggtggcaa agtgggtctt
     5521 cgccacctca tccagtcctt cttcccccaa ttcctcgacg atccgccgac cagcccgaac
     5581 aacgtgcgtc gcgccgagcg gcaatttgaa cccgacccgc tccgacagcc ggtccatctc
     5641 gtcgagctgg cgcgcacgcg ccacgatgga ggcggcggca acagccgcgt cgtcctcggc
     5701 ccgcgtgcgt acctccagcc gcacgccctc aggcacgaac ggctcaaggt gggagcgggc
     5761 agacctggca aactcgtcca cgactaccag gtcaacctca ttcgcaagtt cacctatgat
     5821 ctcggcgtcg agttcgccga gcagcctgtt gacgtttccc gcggagcgcc gcctggtctc
     5881 atactcccga ggaggcaatg atctcacaca gacgttgtcc tcacccacgg tttcgaggat
     5941 gcgatccgcg atcctacgta tgccgaggac actgacggtc ttcgagtccc gaacgcctat
     6001 ctctcgcaga cttttcgcct cttcttcgcc agttatacgg actccggcca caaccagagg
     6061 gccgaagtag tcgcccttcc cggactcgtc gatacccagc ctcggcgtac cgtcgagaac
     6121 gggccgccct ccggcgggag gagtgaaaac ccgtctattt tcacccttcg tttgcgacgt
     6181 gcgccagcct tcgagcaggt cccgcaaccc tgaagctcga cctccggtag atatcttgcc
     6241 cgtatgataa acgttcagct tcgcggtctc tccggcgcgg gtgagatcgt actgggtgcc
     6301 gtggtctatg ggcctgctgc ctgctatctc gatgcc
//
LOCUS       DATAIR010000357         7071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_23083, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000357 DATAIR010000000
VERSION     DATAIR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23083"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VHM69_08730"
     CDS             complement(<1..1136)
                     /locus_tag="VHM69_08730"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX2740517.1"
                     /translation="MAHKPSVHIEFRDEVWTVVREGNKRATSTHPTQSEAAESGRDLA
                     RRDKSEFFLHAQDGRIREHRDYRDEPDKGLLDQTSETVGAVTGALTGTTGAATHALGT
                     GDERDKTEPIAEDDQASSDTNADANIETSNLDVDDTREVRGLTDAEREDPSLAPEQRY
                     ADYEIFDQDSEKIGPLNELFVDEGDEPEYVGVGTSSPDNRSVLVPVEAVTVDDALHRM
                     VISRPRTFVEAGPSLGYEDEVTPEVERRVRVHYGLETSQDAEVRAGYGAYYPGDAVLT
                     ELDSGDIPETGAPEDEVRVQRAEEEIRVETREREAGAMRVRKRVRTETERIEVPKKRV
                     EVTVERVPVEGGSITAGEGATTAPEIGEEEIVVPVIEEEILVEKR"
     gene            complement(1176..1511)
                     /locus_tag="VHM69_08735"
     CDS             complement(1176..1511)
                     /locus_tag="VHM69_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX2740518.1"
                     /translation="MGRRWRDKPEEVVDRGVGRVADAAGSVTNDESLESEGRALGRTP
                     DMETYRVVAQPDGGWIVENGTSGQVSSVHRTKDEAVQDARGRAKAHEPSQVLVYKKNG
                     TVQSERTYG"
     gene            1671..2726
                     /locus_tag="VHM69_08740"
     CDS             1671..2726
                     /locus_tag="VHM69_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740519.1"
                     /translation="MEYRQLGHSGLFVSAMTLGTMTFGGRGDFGMVGSADAASATRQV
                     DMCLDAGVNLFDTADMYSDGESEEILGQAISGRRDRLLLATKARMPVGEGPNDAGLSR
                     HHLIRQCEASLKRLGTDYIDLYQTHEWDGVTPLEETLDALDTLVRSGKVRYVGCSNHS
                     GWQLMKALGVSERLGLQRYVSQQIYYSLEARDAEYELVPLAIDQDCSILVWSPLAGGL
                     LSGKYRRDRDASEGRHVSGWDEPPVRDPDKLYDTIDTLVEIADGHGVSAAQVALAWLL
                     GRPGVASVVVGARTEDQLADNLAAAGSRLSDEERRRLDDVSAPPLLYPYWHQARTASD
                     RLSPADLTLLGPHLVDG"
     gene            complement(2744..3616)
                     /locus_tag="VHM69_08745"
     CDS             complement(2744..3616)
                     /locus_tag="VHM69_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2740520.1"
                     /translation="MAELHRPIVPYVRDASFPVRAAWYTAPRRLLDYLLMYVQEGEMI
                     ADVEGRRHRLHAGDFCLIQPGELHTLEGPAPTITPYVHLDVFYNPARERSFVVRSGRV
                     DVSGLEELMQPRLHEATGIRVPTRFVPLHPAEFRDTMLKTIGIWQRRDLLGQLESHYL
                     ATGLVLSVLQSYARRSTESLGQPEFLNWITSFMSLHLSEPLSVSDMAEHAGLSASRFS
                     QVFRDRFGRPPHQFLLHLRIQRAQDLLQHTGLTMREISVQCGFSDVHHFAKTFRRLSG
                     QTPGSYRSANQQSA"
     gene            3887..5263
                     /locus_tag="VHM69_08750"
     CDS             3887..5263
                     /locus_tag="VHM69_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2740521.1"
                     /translation="MYGEVSRRQFLVRGGKAALGAGLAGSLLAACGGGGGGSGQVTYW
                     SNLEGSGPQDYFKKNIEKPFEKAEDGTDLKVTFQPPDEMDRLVRTALQGGEGPDIIMT
                     PGPAYAQEYIDANLFAELDEYADQYKWDDKLLGWALDLGRFEGKLYSIPYQLQTMLLY
                     YNKTLFEEQGMQPPKSREEFEAMAEELQGQGITPVAAGIGDDPAAIEWFPTVFWNHYS
                     GPEALYQALTGEIPFSDPIFVEAIELFNTYVQKGWFGGSREKFFSNGFDALHADLGDG
                     KAGTNIEGSWFLSTINDYFDGTGNDWDWAPLPALRSGIPAELYELGIGSTLSINQRAQ
                     NADGAAAFIDYLAADKKQAAEWMAAVPAAFNAPLPFTESDFPSSMDDRVARQLAALSQ
                     ATDKGNFGYTAWTFWPAKSDVYIQEESQKVLTGDITAAEFCDGLDEVFTQDRKEGAVP
                     KIIKPGTA"
     gene            5263..6201
                     /locus_tag="VHM69_08755"
     CDS             5263..6201
                     /locus_tag="VHM69_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2740522.1"
                     /translation="MEANHTTITAPDGAAKRDPGRGLRRRRLMTGWLFMLPLIVVNVL
                     VIIGPSVATVYYSFTEWSGIGPAEFVGLQNYRDIVADGDFWSALLHNLIWTVIFLTVP
                     IAMGLLGAFLLSQITRFQMVFRVLYFIPYVMASVVNAALWQNILDPAQGIGSVLATVG
                     IPFLDGVSFFGSERLALPAVAFVDNWHWWGFVVLLFLTALQSVDKELYEAARMDGANR
                     WQQFINVTIPGIRATLVFVVLMTIIGSLLVFDYIYIITQGGPAGASEVVGTLMYKEAF
                     ARFEAGYAAALGLGMSFMSGLVVLIFLYLRRRGWEV"
     gene            6198..7049
                     /locus_tag="VHM69_08760"
     CDS             6198..7049
                     /locus_tag="VHM69_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2740523.1"
                     /translation="MSTLTRGRRQRREPSKVPYYIVLILLALFAIGPLVVLVFNSLKT
                     NAEIGRNPMGPPLSPVFENFPTAWSEGNFATTLINSTILTTGTIVGVCIIAGTAAYAM
                     ARLDVPGTDGVLLYLFVASALPFQLFIVPLFFLWSMLQLTNTLFGLIVIYWAIFSPFA
                     TLLLRSYLVALPRGFEDAARVDGATELQVLLRVVLPLSWPGFLTIALVSGLGAWNEFF
                     FAITFIQDENLKPVTTSFLAFRSSFSQEWGLTSAASIFVILPVVVLFLGLQRRFINGL
                     TAGGLKG"
BASE COUNT         1224 a         2255 c         2167 g         1425 t
ORIGIN      
        1 cgcttctcca ccaagatctc ttcctcgatc acgggcacca caatctcctc ttccccgatc
       61 tcgggcgcag tagtggctcc ctccccggca gtgattgacc ctccttcgac ggggacacgc
      121 tccacggtta cctcgacgcg ctttttgggt acctcgatcc gctccgtttc agtgcgcacg
      181 cgcttgcgca ccctcatcgc ccccgcctcg cgctcgcggg tttcgacccg gatctcctcc
      241 tcggccctct gcaccctgac ctcgtcctcg ggggcgccgg tctctgggat gtcaccgctg
      301 tctagctccg tcagtaccgc atcgccggga tagtaggcac catagccggc tctgacttcg
      361 gcgtcttgtg aagtttcgag cccgtaatgc acgcggaccc tccgctcgac ctcgggtgta
      421 acctcgtctt cgtagccgag gctcggtccg gcttccacga aggttctggg gcgtgagatc
      481 accatgcggt gcagcgcatc atcgaccgtg acggcctcca ccgggacgag gacagacctg
      541 ttatccggcg agctcgtccc tacgcccacg tattcgggtt cgtcgccctc gtcgacgaat
      601 agctcgttca gaggtccgat cttctcgctg tcctggtcga agatctcgta gtccgcatac
      661 ctttgttcgg gtgccagaga agggtcttcg cgctccgcgt cggtcaagcc gcgcacctcc
      721 ctggtatcgt caacgtccag gttggatgtc tcgatgttcg cgtcggcgtt ggtgtcgctg
      781 gatgcctggt catcctcggc tatcggctct gtcttgtccc tttcatcgcc tgtcccgaga
      841 gcgtgcgtcg cggcgccggt ggtcccggtg agagcgccag taaccgctcc gactgtctca
      901 gaggtttgat ccaggagccc cttatccggc tcgtccctgt agtcacggtg ctccctgatc
      961 cttccatcct gagcgtggag gaagaactcg ctcttgtcgc gacgggcgag atccctgccg
     1021 ctctcggcgg cctcagactg ggttggatgg gtagacgtcg cccgcttgtt gccctcgcgg
     1081 actacggtcc atacctcgtc ccgaaactcg atgtgcacgg agggtttgtg cgccatatcg
     1141 tcgactccct ttcggtttgc cttcgcgagg acctgctatc cgtaggtgcg ttcggattgc
     1201 acggtcccgt tcttcttgta taccaggacc tggctcggct cgtgtgcctt ggccctcccg
     1261 cgggcgtcct gcacggcctc atccttcgtg cggtggacgc tggagacctg gccggaggtc
     1321 ccattttcga ctatccaacc tccgtccggc tgggcgacca cgcggtaggt ctccatgtcc
     1381 ggcgtcctgc cgagagcacg gccctcggat tccaggctct cgtcgttcgt cacggagccg
     1441 gccgcatcag caacccggcc cacgccccta tctactacct cttcaggttt gtctctccat
     1501 ctcctgccca tgacccgcct tcttccttga ttggtccatc tatcctcgtt agatgaacga
     1561 ggcatccaca tagttacgcg agactttctc caagactcca cccccgcgcc gcagtctcga
     1621 cctcatcgtg ctaggctgac gcgccaacaa cggctacatg agaggaccac atggagtatc
     1681 gtcaactagg gcactccgga ttgtttgtct ccgcgatgac cctcggcacc atgacgttcg
     1741 gcggtcgtgg cgactttggc atggtcggct ctgccgacgc cgcttcggcg acccgccagg
     1801 tggacatgtg tctcgatgcg ggcgtcaacc tcttcgacac ggcggacatg tactcagacg
     1861 gcgagtcgga ggagatcctg gggcaggcga tctccgggcg ccgcgacagg ctgcttctgg
     1921 ctacgaaggc gcgcatgcca gtgggggagg ggccgaacga cgccgggctc tccagacacc
     1981 acctgatccg gcagtgcgag gcgagcctga agcgcctcgg tacggactac atcgacctct
     2041 accagacgca cgagtgggac ggcgtgacgc ctctggagga gacgctggat gcgctcgaca
     2101 ccctcgtacg ttctggcaag gtgcgctacg tcggttgctc gaaccactcc ggctggcagc
     2161 tcatgaaggc cctcggcgtc tccgagcgtc tcgggctgca gcgttacgtg agccagcaga
     2221 tctactactc cctcgaagca agggacgccg agtatgagct ggtgccgctt gctattgacc
     2281 aggattgctc gattctcgtc tggagcccgc tggccggcgg gctcctctca ggcaagtaca
     2341 ggcgagaccg ggacgcctcc gaaggacgcc acgtaagcgg ctgggacgag ccgcctgtgc
     2401 gcgaccctga caaactctac gacaccatag atacgctcgt tgagatcgcc gacggacacg
     2461 gcgtctcggc cgcccaggtc gccctcgcgt ggctcctcgg ccgccccggc gtcgcttcgg
     2521 tcgtggtcgg tgcgcgcact gaagaccagc tcgcggacaa cctggcagcc gccggatcgc
     2581 gcctctcaga cgaagagcgc cggcgcctgg acgacgtaag cgccccgccg cttctctatc
     2641 cctactggca ccaggcaagg acggcctcgg accgtctcag ccctgccgac ctgacgctgc
     2701 tcggtccaca tcttgtcgac ggatagatag cgcgttgggc tgctcaagcg gattgttggt
     2761 tggcgctgcg gtaggaacct ggagtctggc ccgagagtct cctgaacgtt tttgcgaagt
     2821 ggtggacatc ggagaagccg cactggacgg agatctcccg catcgtcagg ccggtgtgct
     2881 ggagcaggtc ctgggcgcgt tggatccgca aatgcaacag gaactggtgc ggcggcctgc
     2941 cgaagcggtc gcggaagacc tgcgagaacc gggacgccga gagcccggcg tgttcggcca
     3001 tgtcggagac cgatagcggt tccgagaggt gcagcgacat gaacgaggtg atccagttaa
     3061 ggaactccgg ttgcccgagt gactcggtgc tcctgcgggc gtagctctgg agcaccgaca
     3121 gaacgaggcc cgtagcgaga taatggctct ctagctggcc gagaaggtcc cgcctctgcc
     3181 agatgccgat ggtctttagc atggtgtccc tgaactccgc gggatgtaaa gggacgaacc
     3241 tggtgggaac ccgaatcccg gtcgcctcat ggagcctcgg ttgcatgagc tcctcgagac
     3301 ccgaaacgtc cacccgtccg gaacggacca cgaagctgcg ctcccgcgcc gggttgtaga
     3361 agacgtcgag gtggacgtag ggggtgatgg tgggggccgg tccctccagg gtatgtagtt
     3421 cgccaggctg gatgaggcag aagtcccctg catgaagccg gtgcctgcgc ccttcgacgt
     3481 cggcgatcat ctctccttcc tgcacgtaca tcaacaggta atctaatagg cgccgagggg
     3541 ccgtgtacca cgcggccctt acgggaaagc tggcgtcacg aacgtatgga acgatgggtc
     3601 tgtgcaactc cgccatcctg tcatccacct cgacttcgat cagatcagca gtcgtttttt
     3661 acaacaagtt agtgtctgta agcaatgatc caagccctcg aatggcgtaa tatgaaagtg
     3721 aaacaggtgg gagtggagga aacgctcgca gaagggcgtg tatgtacgag gtaaaaatca
     3781 acaacgccgt tgtccggcga cccgcgattc ctcgtgtccg taaggaggac atcttcgcgg
     3841 gtcgtctctt tctcttatcg tgtcgtcgtt cgaaagggag ttaagcatgt acggagaggt
     3901 atcgcgcagg caattcctgg taagaggagg caaagcggcc cttggggccg gtctggctgg
     3961 ttcgctgttg gcagcgtgtg gtggcggcgg gggtggaagc ggccaggtca cgtactggag
     4021 caacctcgaa gggtccgggc cgcaggacta cttcaagaag aatatcgaga agccgttcga
     4081 gaaggccgag gacggcacgg acctcaaggt cacgttccag ccgcccgatg agatggaccg
     4141 gctcgtccgc acggcgttgc agggtggtga ggggcccgac atcatcatga ccccgggtcc
     4201 cgcctacgcc caggaatata tcgacgcgaa cctcttcgcc gagctcgacg aatacgcaga
     4261 ccagtacaaa tgggacgaca agctgctcgg atgggcgctc gacctcgggc gcttcgaggg
     4321 caagctgtac tccatccctt accagctcca gaccatgctt ctctactaca acaagacact
     4381 gttcgaggaa cagggcatgc agccccccaa gagccgcgag gagttcgagg cgatggccga
     4441 ggagttgcag ggccagggca tcactcctgt tgccgcgggt atcggggacg accccgcggc
     4501 gatagagtgg ttcccgaccg tcttctggaa ccactactcc ggacccgagg cgctctacca
     4561 ggcgctgacc ggtgagatcc ccttctcaga cccgatcttc gtcgaggcca tagagctatt
     4621 caatacctac gtccagaagg gctggttcgg gggaagccgc gagaagttct tctccaacgg
     4681 cttcgacgcc ctgcacgccg acctcggaga cggcaaggcc ggcacgaaca tagagggctc
     4741 gtggttcctc tcgactataa acgactactt cgacggtaca ggcaacgatt gggactgggc
     4801 gccgctgccc gcgttgcgga gcggaatccc cgcggagctc tacgagctcg gcatagggtc
     4861 tactctgtcc atcaaccagc gtgcccagaa tgccgacggc gccgccgcgt tcatcgatta
     4921 cctcgccgcg gataagaagc aggcggccga gtggatggcc gccgttcctg cggccttcaa
     4981 cgcgcccctg cccttcaccg aatcggactt cccttcgagc atggatgaca gggtcgcgag
     5041 gcaactcgcg gccctgagcc aggctaccga caaaggtaac ttcggctaca ccgcatggac
     5101 cttctggccg gccaagtccg acgtgtacat ccaggaagaa tcgcagaaag tcctcacagg
     5161 ggatatcacg gctgccgagt tctgcgacgg actcgacgag gtctttacgc aggaccgcaa
     5221 ggaaggcgcg gtgcccaaga tcatcaagcc aggaaccgcc tagtggaggc aaaccacact
     5281 acgatcacgg ccccggacgg cgccgcgaag cgggatcccg ggcgaggtct caggaggagg
     5341 cggctcatga ccggctggct cttcatgctc ccgctcatcg tggtcaacgt gctcgtcata
     5401 atcgggcctt ccgtcgccac cgtctactac tcgttcacgg agtggtcggg gatcggccct
     5461 gccgagttcg tgggtctgca gaactacagg gacatcgtcg ccgacgggga cttctggtca
     5521 gcgttgttgc acaacctgat ctggacggtc atcttcctga ccgtccccat cgcgatgggg
     5581 ttgctggggg cgtttttgct atcccagatc acccgcttcc agatggtgtt ccgcgtcctg
     5641 tactttatcc cctacgtgat ggcgagcgtc gtgaacgccg cgctctggca gaatatcctg
     5701 gatcccgcac agggtatcgg ctctgtgctc gcgacggtgg ggattccctt tcttgacggg
     5761 gtctcctttt tcgggagcga gaggctcgcg ctgcctgcgg tcgccttcgt ggataactgg
     5821 cactggtggg gcttcgtcgt actgctgttc ctgaccgccc tgcaatccgt ggacaaagag
     5881 ctctacgagg cggcaagaat ggacggggcg aaccgctggc agcagttcat aaacgtcacc
     5941 atacctggca tcagggcgac gctcgtcttc gtcgtcctca tgacgattat cgggtcgctt
     6001 cttgtcttcg actacatcta catcatcacc cagggcggac ccgccggggc gagcgaggtc
     6061 gtcggcaccc tcatgtacaa ggaggcgttc gcgcgcttcg aggccggcta cgctgcggcc
     6121 ctcgggctcg gaatgagctt catgagcggt ctggtcgtcc tgatcttcct gtacctgcga
     6181 aggagggggt gggaggtgtg agcaccctga cgagaggacg ccgtcagaga cgggagccgt
     6241 cgaaggtccc ttactacatc gttctcatat tacttgcgct cttcgccatc gggccgctgg
     6301 tggtgctcgt gttcaactcg ctgaagacga acgccgagat cggacgcaac ccaatgggac
     6361 ctcctctctc cccggtcttc gagaacttcc cgaccgcctg gtcggaaggg aacttcgcga
     6421 cgaccctcat aaacagcacg atcctgacca caggcacgat cgtgggggtt tgcatcatcg
     6481 ccggcacggc cgcctacgcg atggcccgtc ttgacgtgcc gggcacggac ggagtgctgc
     6541 tatacctctt cgtggcgagc gcgcttcctt ttcaactctt cattgtgccg ctgttcttcc
     6601 tgtggagtat gctccaactc accaacacgc tcttcggcct gatcgtgatc tactgggcca
     6661 tattctcgcc gttcgcgacg ctgctcctgc gctcgtacct ggtggccctg ccgcgaggct
     6721 tcgaggacgc cgcccgtgta gacggggcga cggagctaca ggtgctgctc cgcgtggtac
     6781 tgcccctgag ctggcccggc ttcttgacca tcgccctcgt ctccgggctc ggggcgtgga
     6841 acgagttctt cttcgccatc acctttatcc aggacgagaa cctcaaaccc gtcacgacga
     6901 gcttcctggc cttccggagc agctttagcc aggagtgggg cctgaccagc gcagccagca
     6961 tcttcgtcat acttcccgtc gtggtcctgt tcctgggcct ccagcgccgc ttcattaacg
     7021 ggctgacggc gggtggtctg aagggatgac gccgcggatg ttccggtact c
//
LOCUS       DATAIR010000358         8434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_300527, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000358 DATAIR010000000
VERSION     DATAIR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300527"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHM69_08765"
     CDS             complement(<1..355)
                     /locus_tag="VHM69_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX2740524.1"
                     /translation="MSDRARRIANLSPEKRRLLESMLQERAQGRADEGIPTLPRYSES
                     GQPRVFPLSSAQRLMWFNIQRHPRVHKMPPRAMRLRGPLDVAALEASLHEICARHEAL
                     RTTFGTRDGEPVQIVG"
     gene            complement(352..5535)
                     /locus_tag="VHM69_08770"
     CDS             complement(352..5535)
                     /locus_tag="VHM69_08770"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2740525.1"
                     /translation="MNDDRSGLPIDLRYPEQVRRVHPTNAFIRFEKEETEQSIPQRFE
                     QQVRRYPDRLAVKTKGHQLTYAALNKVANRMARVLLARRGEGPESIALLLEHNASMIG
                     AILGVLKAGKAYVPLDPSFPYARNAYILEDSQAGLIITNDKNRPLADSLGENRHSLID
                     IDEIGPALPDEDANLPISADNLANIIYTSGSTGQPKGVVQNHRNLLNVAMRYTNGLQI
                     SAEDRLTLLQSYSVAGSVSNMLGALLNGASLFPHNIKEEGLTSLADWLIEEEITVYHS
                     VPTVFRQFANTLTGKEEFPWLRLVRLGGEPVYPEDVQLYKTYFPSGSIFVNSYGASEA
                     ASVLRYCVDKDTEISDAMVPVGYPLGDIEILLLDDDGKAVGFDEVGEIAIKSRHISPG
                     YWHKPDLTRATFITDPQDEGGRIYRTGDLGYRQPDGCLVVTGRNDFRVKIRGFRIEVA
                     EIELALRGLTKVKEAAVVAHEDQRGEKQLAAYVVPQPDHMPTTSELRDFLKDKLPDYM
                     VPSAFVVLDALPLTPNGKLDRLALPAPSLVRPELDTSFVAPRNALEEQLVEIWVEVLG
                     VTRVGVHDDFFELGGHSLRATQLVSRVREVFQAELPLISLFEEPTIAGLAERIEEARR
                     GAQGQTAPPLVPAPRDARLPLSFSQQRMWLLDQLEPGTPTYHISHALRLSGTLDTGAL
                     KRSLTEIVSRHEALRTTFAAEDGEPVQVISATIDVKLPVEDLSGLPQAEQEAEAKRLA
                     LEEKRRPFDLERGPLFRAGLLRLGKEEHVLLISMHHIVSDGWSMGVFWRELGALYGAF
                     SGGEPSPLPGLPIQYADYALWQRQWLTGEVLEEQLGYWKEQLADLPVLELPTDHPRPA
                     VQTHRGARRSLAMPESLTQALKELSRQEGTTLFMVLLGALQVLLSRYSGQEDIAIGTP
                     IAGRTRAETEELIGFFVNTLVMRTYLSGDPSFREVLSRVRGVALGAYDHQDLPFEKLV
                     EELQPERDLSRVPFSQVFFALQNVPQEALKLPNLVPEQQKVESGTVKYDLSFFLSEEE
                     QGLRGRLVYNADLFDDATIERMLGHLQTLLKGIVEDPDRHLSELPLLTEAERHQLLFE
                     WNETATEYPWDRCVHELFEEQAGRTPDAVAVVFEEEQLTYRELNRRANQLAHHLRALG
                     VGPEVLVGICVERSLEMVVGLLGILKAGGTYVPLDPSYPATRLKFILGDAQAPVLLTQ
                     ERFVEGLPDKHGAEVVRLDADWPLIARNAEENVRGRATADNLAYVIYTSGSTGQPKGV
                     MIEHRALSSYVAAAIAAYEITASDRVLQFASLSFDASVEEIFPSLACGGTLVLRSGRM
                     VDSMQRFVRECTECAISVLDLPTAFWHELVLAFESEGVVLPPSVRLVIIGGEKALAER
                     VAQWHTVQTARPARLINTYGPTEATVVATHCELRPGASHDLVTGGEVPIGRPLGNALI
                     YILDGSLNPLPIGVPGELHVGGPGLARGYVNRPELTSERFIPDLFSDAPGARLYKTGD
                     VARWRRDGNIEFLGRVDDQVKIRGFRVEPAEVEAVLGRHPALRGTAVLARMDAFGDER
                     LVAYAVPHGRPAPTARELGDYLKERLPDYMVPSAFVTLDSLPLTPSNKVDRRALPDPD
                     LSRFRAENAYAEPRTPVEEQLVEIWEEVLGIERVGIQDGFFELGGHSLLATRVVSRLN
                     RHFGVELPLRVLFEDPTIEGLALAVTQMQAEAEIDIEELLAQVEQMQDQPVSEEFSWE
                     Q"
     gene            complement(5912..7771)
                     /locus_tag="VHM69_08775"
     CDS             complement(5912..7771)
                     /locus_tag="VHM69_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2740526.1"
                     /translation="MRRSLSSAVTPGKSAPADSSTIRSYIGALVDVASWKIVFVSLLM
                     VCVTLTEGVGLLLLVPLLQVVGLDMGRSTVGRISEYITSIFDAVGARPTLVLVLGLYV
                     LVISARAFASSWQTVVSTAIAHDFKVHLRQRLYRAIVNTDWLFFSRSRSSDFTHVLTT
                     ELDRVGRATRQLLRFIAEVGVAAVYILVAVFLSPTVSALAFVCAAGLMLLVRRTMQAT
                     RAGMKEASRATKGLYAAANEQLSGTKTIKSFGAEERNVAIFSRFTERIARMYIDAARH
                     GAEASRWLNIGMVLVLSLIVYVSIEILATPASQLLLLLYLFAMITRMLSRVQTHYHEF
                     IVESQAFTTVTEMEARCVAAAEPEPARYEGIELRGAARLEGVSFAYQRSGEPLVISEL
                     ELTIPAGKTTAIVGPSGAGKSTIADLIMGLIQPNKGRVLVDEMPLRTEQRRAWRSKIG
                     YVPQDTFLFHDTVRENLLWARPGASDGEIREALRLAAADDFVSRLPEGMETVLGDRGM
                     RLSGGERQRLSLARALLRKPSLLILDEATSNLDSENERRIQSAIEELHGSMTILIITH
                     RLFTTRSADVIYVLEQGRLVESGAIDALVAREQGRFLELCKAQSIDTVTLVGN"
BASE COUNT         1644 a         2658 c         2457 g         1675 t
ORIGIN      
        1 gcccaacgat ttgcaccggc tccccatcgc gcgtgccaaa agtggtacgc aacgcctcat
       61 gacgtgcgca gatctcatgc aggctagctt ccaacgccgc gacatcgagc ggaccacgca
      121 aacgcatcgc ccgcggcggc atcttatgaa ccctcgggtg ccgctgtata ttgaaccaca
      181 tcaaccgctg ggcggaggag agcgggaaaa ccctcggctg gccactctcc gaatagcggg
      241 ggagcgtcgg gatgccctcg tccgcgcgcc cctgggcgcg ctcctggagc atcgattcca
      301 gcagccggcg tttctcggga gacaggttgg cgatgcgccg cgccctgtcg ctcattgctc
      361 ccaagagaac tcttcagata cgggctggtc ctgcatctgc tctacctggg ccagcaattc
      421 ttcgatatcg atttccgctt ctgcttgcat ctgcgttacg gccaacgcca acccctctat
      481 agtggggtcc tcgaacagga cgcgcagcgg caattcgact ccgaagtgcc ggttgagccg
      541 cgagacgacc cgcgtggcca gcagcgagtg cccccccagc tcgaagaagc cgtcctggat
      601 gccgacccgc tctatcccca atacctcttc ccagatctct acgagctgct cctctaccgg
      661 cgtgcgcggc tccgcatagg cgttctccgc tcggaagcgg gatagatcgg ggtccggcag
      721 cgcacgccga tcgaccttgt tgctaggcgt caggggcagg ctatccaatg tcacgaaggc
      781 cgagggcacc atgtagtcgg gcagcctctc cttaaggtag tcccccagct ctcgtgccgt
      841 cggtgccggt cgcccatgcg gaactgcgta agccaccaaa cgctcgtcgc caaaggcatc
      901 catacgggcc aggacagcgg tccctcgcaa agccgggtgc cgccccagta ctgcctcgac
      961 ctcggccggc tcgactcgga agccccggat cttgacctgg tcgtcgacgc gtccaagaaa
     1021 ctcgatgttc ccgtctctgc gccagcgcgc aacatctccg gtcttataga gtcgcgcccc
     1081 tggcgcatcg ctgaagagat ccgggataaa tcgctcggag gtcagctcgg gacggttgac
     1141 gtacccgcgc gccaacccag gaccgccaac gtgcagctcc ccgggcacgc cgatcggcaa
     1201 gggattcaga ctcccgtcca ggatgtaaat aagggcgttg cccagaggcc gcccaatcgg
     1261 cacctcgccc ccagtcacaa ggtcatgact ggctccgggg cgtaactcgc aatgagtcgc
     1321 gactaccgtg gcctcggtcg ggccatacgt attgatcagg cgcgccggac gggcggtctg
     1381 caccgtgtgc cattgggcta cgcgctcggc cagcgctttc tcaccgccga tgatcacaag
     1441 gcgcaccgac ggcggcaaga ccacgccttc gctctcgaaa gccagtacta gctcgtgcca
     1501 gaacgccgtg ggcagatcca acacgctgat cgcgcactct gtacactcgc gcacgaaccg
     1561 ctgcatcgag tcgaccatcc ggccactccg cagcaccagc gtgccgccgc atgccagaga
     1621 ggggaagatc tcttcgacgc tagcgtcgaa gctcaaagag gcgaactgaa gcacgcggtc
     1681 agatgcagtt atctcatagg ctgcgatggc cgccgcaacg taactgctta gtgcacgatg
     1741 ttcgatcatc acgcctttgg gctggcccgt cgaacccgag gtgtagataa cgtacgcgag
     1801 gttatcggcc gtggcgcggc ctctgacgtt ttcctcggca ttccgggcga tgagcggcca
     1861 gtctgcgtcc aggcgcacta cttcagcccc gtgcttgtcg ggtagcccct cgacaaaccg
     1921 ctcctgggtc agcagcaccg gcgcttgagc atcccccagt atgaacttca agcgcgtggc
     1981 ggggtacgaa gggtccagcg gaacgtaggt gccacccgcc ttcaggatgc ccaacagccc
     2041 caccaccatc tccaaagagc gctcgacgca gatacctacc aagacctccg gtcccacgcc
     2101 cagcgcgcgt aggtgatgcg cgagctggtt cgcgcgtcgg ttgagctccc ggtaggtgag
     2161 ttgttcctcc tcgaatacga ccgcaacggc atcgggggtg cgtccggcct gctcctcgaa
     2221 gagctcgtgc acgcaccgat cccacggata ctctgttgct gtttcgttcc actcaaagag
     2281 cagttgatgt cgctccgcct cggtcagcag cggcagctcg gagaggtgcc gatcggggtc
     2341 ttccacgatg cctttcagga gcgtctggag atgacccagc atccgttcga tagtggcatc
     2401 gtcgaacagg tcggcattgt ataccaggcg gcccctcaaa ccttgctcct cctcggacaa
     2461 aaagaagctc aggtcgtact ttaccgtacc gctctcgacc ttctgctgct cgggtaccag
     2521 gtttggaagc ttcagggcct cctgcggcac attctgcagc gcaaaaaaca cctgggagaa
     2581 gggcacgcgg ctcagatctc gctcgggttg cagctcctct accagcttct caaagggaag
     2641 gtcctggtgg tcgtacgccc ccagtgctac gccccttacg cgagagagta cctctctgaa
     2701 gctcgggtct cccgaaaggt aggtgcgcat aaccaatgtg ttcacaaaga agcctataag
     2761 ctcctcggtc tcggcccggg tgcgtcccgc gataggggtg cctatcgcta tatcctcctg
     2821 ccccgaatag cgtgagagca aaacctgaag ggcaccgagg agcaccatga agagcgtggt
     2881 cccttcctgc cggctcaatt cctttagcgc ctgggttagc gactcgggca tcgcgagcga
     2941 tcggcgagcc cccctgtgag tttgcactgc cgggcggggg tggtcggtgg ggagctccag
     3001 gacgggcagg tcggccagtt gctccttcca gtagccgagc tgctcctcta gcacctcccc
     3061 cgtaagccac tgcctctgcc acagcgcgta gtcggcgtac tggatgggta gccccggtag
     3121 cggcgaaggc tcacctccgg agaaggcccc atagagcgct ccaagctcgc gccagaacac
     3181 acccatagac cagccgtcgg agactatgtg gtgcatgctg atcagcagca cgtgctcttc
     3241 cttgcccagc cgcagcagcc cggccctgaa cagcggcccg cgctccaggt cgaagggacg
     3301 gcgcttctcc tcgagagcga gccgcttcgc ctcggcctct tgctcggctt gcggcagccc
     3361 gctcaagtct tcgacgggta gctttacgtc gattgtcgcc gagatcacct gcaccggctc
     3421 accgtcctcg gcggcaaagg tcgtgcgcag agcttcgtgg cgagagacga tctccgtgag
     3481 gctccgcttg agcgcccctg tgtcgagggt gccactgagt cgcagggcgt gggagatgtg
     3541 ataggtgggg gtgccgggct ccagctggtc gagcaaccac atccgctgct gagaaaacga
     3601 cagcgggagc cgggcgtctc gcggggcggg tactagcggc ggagccgtct ggccctgcgc
     3661 gcctctgcgt gcctcctcga tcctctcggc aagccctgct atggtgggct cttcgaagag
     3721 gctgatcagg ggcagctccg cctggaaaac ctcgcgtacc cgcgagacga gctgggtggc
     3781 ccgcaaggag tggccgccca gctcgaagaa gtcgtcatgg acgccaaccc gcgtgacgcc
     3841 tagcacctcg acccagatct ccaccagctg ctcttcgagc gcgttgcgcg gtgccacgaa
     3901 actcgtgtcc aattcaggcc ggaccaagct cggggcagga agagccagac ggtccaactt
     3961 accgttgggc gtcagcggca gcgcatccag caccacgaac gcggatggca ccatgtagtc
     4021 aggcagcttg tccttgagaa agtcgcgcag ttcacttgtc gttggcatat ggtccggttg
     4081 cggaactacg taagccgcca actgtttttc gccgcgctgg tcttcatgtg ccaccacggc
     4141 ggcttcctta accttggtca gaccgcgcag agccagctcg atctcggcga cctcgatcct
     4201 gaagcccctg atctttaccc gaaaatcgtt acgccctgtc accaccaggc agccatccgg
     4261 ctgcctgtag cccaggtctc ccgtgcgata gatcctcccg ccctcatctt gggggtcggt
     4321 tataaaagtc gcccgtgtga gatccggttt atgccaataa ccaggggaga tgtggcgact
     4381 cttgatcgcg atttcaccga cttcatcgaa tcctaccgct ttgccatcgt cgtcgagcaa
     4441 taggatttct atgtccccga gcgggtatcc gaccggcacc atggcatcgc taatctcggt
     4501 gtccttgtcg acgcaatacc gcagcacact ggccgcttcg gaagcgccat acgaattgac
     4561 gaagatagaa cccgacggga agtaagtctt gtatagttga acgtcttccg gatagaccgg
     4621 ctcgccgccc aatctgacca ggcgcagcca gggaaactcc tccttgccgg tcagggtatt
     4681 ggcgaactgg cggaagaccg tcgggacgga atgatagacg gtgatttctt cttcgataag
     4741 ccagtcggct aaactggtta acccttcttc tttgatgtta tggggaaaca gcgaagcccc
     4801 gttgagcaag gcgccaagca tgttcgagac cgacccggcc acgctgtaag actgcagcag
     4861 ggtcagccgg tcctccgcgc tgatttgaag accgttggta tacctcatgg cgacatttag
     4921 taggttgcga tggttttgga cgacaccttt gggctgaccg gtcgagcccg aggtatagat
     4981 gatgttggca agattgtccg ctgatatcgg aaggttagcg tcttcatcgg gaagcgcagg
     5041 accgatctca tcgatgtcga tcagagagtg cctgttctcg cccaacgagt cggccagggg
     5101 acgattcttg tcgttggtta tgatcagacc ggcctgcgag tcctcgagga tgtatgcgtt
     5161 tcgtgcatac ggaaaggagg gatccaaagg cacgtacgcc ttgcccgcct tcaacacacc
     5221 gaggatcgcg ccgatcatcg aagcgttgtg ctcgagaagc agggcgatcg actccggccc
     5281 ctcgccacgt cgggcgagaa ggactcgtgc catacgattg gcaaccttat tcagcgccgc
     5341 ataggtaagt tggtggccct ttgtcttgac tgccagccgg tcgggatagc gtctcacctg
     5401 ttgctcgaac cgctgcggga tcgactgttc ggtctcttct ttttcgaacc ggatgaaagc
     5461 gttggttgga tgaacccgac ggacttgttc ggggtaccga agatcgatgg gaagacctga
     5521 gcgatcatca ttcatcgtag aacctcgtcc ctagcagcat tcatggacta catgttccac
     5581 gattttactt cgtccacata gcccaaattg gcgctcttgg cttctattta accttcattt
     5641 agcttcacgt agttttcctt gtcaaggcca tcttgcacat cggccaatcg gcacattatt
     5701 cgaaacgagg ggcgccggag tctcgtggca gacgcatccg taccaccgaa ggcttcgagc
     5761 gtctcaacca ggagataaga gggtagtgag gatcttcccc aaccggcggg cgcgtccgag
     5821 cctggttacg gctttggcgg tcgagacctc ggaggagtgg ctaaccggta ggcgctaaag
     5881 aactctgcta cgggagcgaa gaggaaagct ctcaattacc tacgagggtg acggtgtcta
     5941 tggactgtgc cttgcacagc tcgagaaatc gcccctgctc tctggccacg agcgcatcta
     6001 tggccccgga ttcgaccaat cgcccttgtt ccagcacgta gataacgtcc gcgcttcggg
     6061 tggtaaacag cctgtgggta ataatcagta tcgtcatgct cccgtggagc tcctctatcg
     6121 cgctctggat gcgtctttcg ttctctgagt cgaggttgct ggtggcctca tccaggatca
     6181 acaaggacgg cttgcgcagc aaggcacggg ccagggacag gcgttgcctc tctcccccgg
     6241 acaggcgcat gccgcggtcg cccagaaccg tctccatgcc ctcaggaagc ctggagacga
     6301 agtcatcggc ggcagccaaa cgcagtgctt cgcggatctc tccgtcgctc gcgcctggcc
     6361 gggcccacag caggttctcc cttacggtgt cgtggaatag gaacgtgtcc tgtggcacgt
     6421 agccgatctt gcttcgccag gccctcctct gctccgttct caagggcatc tcgtccacca
     6481 gtacccgccc cttgttgggt tggatcaatc ccattatcaa gtcggcgatc gtgctcttgc
     6541 cggccccgga cgggccaacg atcgccgtcg tcttccccgc cggaatggtc aattcgagct
     6601 cggagataac gagaggctcg ccactacgct ggtaggcgaa cgagactcct tcgagccgcg
     6661 cggcccctcg cagctcgatt ccttcgtaac gcgccggctc cggctcggcc gcagctacac
     6721 agcgggcctc catctctgtg acggtagtaa atgcttgtga ctcgactata aactcgtgat
     6781 agtgagtctg aaccctggag agcatccgtg tgatcatggc gaacaagtag agtaacagga
     6841 gcagctggct ggccggggtg gcgaggatct cgatcgagac gtacacgatg aggctcagga
     6901 ccaacaccat gcctatattg agccagcgtg acgcctcggc cccgtgacgg gctgcatcga
     6961 tgtacatccg agctatgcgc tcggtgaacc tggagaagat ggccacgttg cgttcctcgg
     7021 cgccgaagct cttgatggtc ttcgtcccgc tcaactgctc gttggctgcc gcgtacaagc
     7081 ccttggtcgc ccgcgaggct tccttcatac ccgcccgcgt tgcctgcatc gtcctgcgta
     7141 ctagtagcat taacccggca gcacagacga atgccaaagc gctcacggtc ggcgacaaga
     7201 ataccgccac caggatatag accgccgcta cccctacctc ggctatgaac ctcaatagct
     7261 gacgggtcgc tctaccgacc cggtctagct cggtggtcag gacatgcgtg aagtccgagg
     7321 accggcttcg ggagaagaag agccagtccg tgttgactat agcccggtac aggcgctgcc
     7381 ttaggtggac cttgaaatca tgtgcgatgg cggtggagac gacggtttgc cagctgctcg
     7441 cgaaggctcg agcactgatg accagtacgt agagcccgag tacgaggacc agcgtcggtc
     7501 gggcgcccac ggcgtcgaag atcgaagtta tgtactcgga gatccgcccc accgtgctgc
     7561 gccccatgtc cagccccacc acttgcagaa gaggcaccaa caacaatagt ccgacgccct
     7621 cggtgagggt aacgcagacc atcaacagtg agacaaacac aatcttccag gacgccacgt
     7681 cgacaagggc gccgatgtag ctcctgatcg tgctactgtc cgcaggtgcg ctcttgccag
     7741 gagtaaccgc gcttgacaga gaccgtctca ccaagcgaat cctcgaccgt taattatcag
     7801 gagccagcaa agtctattgc cttgcgcagc cgtccccgaa ctcactatga cctctctcct
     7861 cctaataaat cttcatacct ccacggcaca ccgcccgagc aatacctgga gtatggtggt
     7921 tcatggagga tgtttacatg ggctgcccgg cctatttctc accagccggc cattttcgcc
     7981 ggccggaaag cacatacgcc ggcggatgtt atggggaccg gccagtagag atcggggagc
     8041 cgcatcgcgg ggccgcaatc tctcgtcgac cgacgtgctc tctcttcggg gcgatacgct
     8101 tggccgtgga cgtggaggcg gaaacgcccc tgtatcaggt agttgatctc ctcgatctaa
     8161 cgggaatgga atcaagtgcc gaatgctctg tagttccccc gaaatggaag attgcctgtt
     8221 tccccataag accacatccg ttcgttctca cgagcgttct tgcttcggtt cctctatgtt
     8281 caagagcacc ccctcgacgg tcctatgact acgtctggcc attgtcttac agaaccttat
     8341 gggcacaacc cgggagaatt cgaggctata catgccctga aggccggaag tagatttggt
     8401 cgctggtccg gaaaatggag tttacaccgc taat
//
LOCUS       DATAIR010000359         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_23392, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000359 DATAIR010000000
VERSION     DATAIR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23392"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="VHM69_08780"
     CDS             complement(<1..1982)
                     /locus_tag="VHM69_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740527.1"
                     /translation="MATIAVALFAAWIMESLQEVAIEERRAQTVLSEFEADMDQLEIA
                     VEVALREGKVTPDTAEELKAERLELKDHIDELKTLNLPEEDTARISEALGKTGAAVDG
                     VLDLIEAGKLDQARSVERERVRPSFEDLDESVEGISEKLEGSARRTEFIAGGGIYAIN
                     LLAAFLLIAVYWWYERRLHAEQAELQNAKEAAEEANRAKSEFLANMSHEIRTPMNGVI
                     GMTGLLLNTKLDREQTEYAEIVRASGESLLTIINDILDFSKIEAGKMELESMDFDLHG
                     VVEESVDLLAERAYARGLELASLVGQGVPTDLRGDAGRIRQILINLLSNAIKFTESGE
                     VVLLVTPVEEDEDAATLRFEVRDTGIGMTEVQKGRLFQSFTQADTSTTRRYGGTGLGL
                     AISKQLVEIMGGEIGVETEPQVGSTFWFTLPLYKQTVGTYKRTDPVPRADLGELRVLV
                     VDDNETNRKILHEQVVPWGMESVAVEDGWEALQALREAANSGEPYDLAMLDREMPNMD
                     GMELATRIKADPSIAATRLIMLSSAAGRGESEEARRIGIEACLSKPVRQSRLYDTIAA
                     LMGSPEAQATTREPEQEAPLLRGEAWDAGFRERRVPVLVVEDNVVNQRVAVKMLQKLG
                     YRADVVANGLEALEALSRIPYSAILMDVQMPEMDGYA"
     regulatory      complement(2227..2321)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2404..3264)
                     /gene="purU"
                     /locus_tag="VHM69_08785"
     CDS             complement(2404..3264)
                     /gene="purU"
                     /locus_tag="VHM69_08785"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX2740528.1"
                     /translation="MGYNLRLLISCPDRRGLIAAVSSFIAMHDGNILSADQYVSDGEA
                     GGTFFMRLEIEGQGFNLARDEFGGAFAPLARKHGMHWRVSYTDTPKHMAILVSRYDHC
                     LMDLLWRWDSGELEAEIPLVVSNHRDLAARVGAYGIPFHYLPVTRETKAEQESEILDL
                     LAEHGVDFVVLARYMQILSPGFVDAYPHRIVNIHHSFLPAFAGADPYRRAHERGVKTI
                     GATAHYVTDELDAGPIIHQDVAHVTHRDTVEDLVRLGSEVERRVLARAVRWHLEDRIL
                     VDGGRTVVFE"
     gene            3459..>5113
                     /locus_tag="VHM69_08790"
     CDS             3459..>5113
                     /locus_tag="VHM69_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2740529.1"
                     /translation="MKRGHNSRQLPREGLSGPQEVVRLKLLGGFRVTIGDRAIAEAGW
                     RLRKAASLVKLLALAPGRRLHREQVMDALWPDLGRRAASNNLRQILHVARKALDPNSA
                     SRCLIYRDGQLLLCPEGPIRVDVDAFEEAAATARRLGEPAGYRAAIDLYAGDLLPADR
                     YEDWAEERRSELRRTFISLLVELASIYEQQREYGPSIETLERAVAGEPLHEEARVALM
                     RVHALAGSPAEALKQYERLEHALYRELGSRPEASSRLLREEIAAGRFPPRETSTTSDS
                     LSAKARTHNLPAVRTKLVGREREMLEIKRELAMTRLLTLTGVGGSGKTRLALEVARDL
                     TGAYPDGVWLAELAPLSEGMLVPGAVAEALGIKERAGQSLVDTLVEAVRPRETLLVLD
                     NCEHVVEQAARLSEVLLDACPGLRILATSREALGVAGEVRWVVPALTTPGLRRSWSVE
                     ELEEYESARLFAERARQRDPSFTLTAARAQPVAQICTRLEGIPLAIELAAARVGMLGT
                     AQISERLEDSIGLLTTTVHTAVPRQRTLRSALDWSHELLSADE"
BASE COUNT          909 a         1645 c         1540 g         1019 t
ORIGIN      
        1 gcatacccgt ccatctctgg catctgcacg tccatgagga tagccgagta ggggatgcgg
       61 gatagagctt cgagtgcttc cagaccgttt gctacaacat ctgccctgta gccgagtttc
      121 tggagcatct tcacggccac cctctggttg accacgttgt cctcgacaac aagcacggga
      181 acacgtcgtt cacgaaagcc agcatcccac gcttcgccac gcagaagggg agcttcctgc
      241 tcgggttctc tggtggtagc ctgcgcttca ggcgaaccca tcaaggctgc tatcgtatcg
      301 tagagtctgg attgcctcac cggcttggaa aggcaagctt cgatgcctat ccgccttgcc
      361 tcctcgctct cgccgcgccc ggcggccgaa ctgagcatta tgagcctggt ggcggctatc
      421 gaggggtcgg ctttgatcct ggttgccagc tccatgccgt ccatattcgg catctccctg
      481 tcaagcatcg ccaggtcgta aggttcgccg ctatttgcgg cctccctcag cgcctggagc
      541 gcttcccacc cgtcctcgac ggcaacgctt tccatccccc aggggacgac ctgctcgtgc
      601 aagatcttgc ggttggtctc gttgtcgtca acgacaagca cgcgcaactc gcccagatcc
      661 gcgcggggca cgggatctgt tcgcttgtaa gtgccgacgg tctgcttgta cagcggcaag
      721 gtgaaccaga aggtgcttcc gacctggggc tcggtctcca cacctatctc tccacccata
      781 atctcgacca gttgcttgct gatggcgagc cctagccccg tgcctccgta ccggcgggtg
      841 gtcgaggtgt cggcctgggt gaaagactgg aagaggcgcc ccttttgtac ttcggtcatc
      901 cctatgcctg tatcccttac ctcgaagcgc aacgtcgccg catcttcgtc ctcttccaca
      961 ggtgtgacga gcaggacgac ctcgccgctt tcggtgaact tgatggcgtt gctgaggagg
     1021 ttgatcagga tctggcgtat gcgtcctgca tcgcccctga gatcggtagg taccccttgc
     1081 ccgacgaggc tcgcaagctc gaggcccctg gcgtaagccc gctcggcgag caggtcgact
     1141 gattcctcga ccacgccgtg caggtcgaag tccatcgatt cgagttccat cttgccggct
     1201 tcgatcttgg aaaagtccag gatatcgttg atgatcgtga gcaggctctc acccgaggca
     1261 cgcactatct ctgcgtactc tgtctgttct ctatccagct ttgtgttaag caagaggcct
     1321 gtcatgccta tgacgccgtt catgggcgtc cttatctcgt gggacatgtt cgccaggaac
     1381 tcgctcttcg ccctgttggc ctcttcggcg gcctccttgg cattctgcag ctccgcctgc
     1441 tccgcgtgca gtcttctctc gtaccaccag tagacagcta tcaataagaa tgcggcaaga
     1501 aggtttatgg cgtagattcc ccctccggca ataaactccg ttcgcctggc agagccttct
     1561 agcttttcgc tgatgccttc cacggactca tcgagatctt cgaagctggg ccgcacgcgt
     1621 tcacgctcta cagaccgggc ctggtcgagc tttcccgctt cgatcagatc cagcaccccg
     1681 tctaccgccg ccccggtctt gcccagcgct tcgctgatgc gcgccgtatc ctcctcagga
     1741 aggttcaatg tcttgagctc gtcgatatgg tccttaagtt cgaggcgttc tgcttttagc
     1801 tcctctgcgg tatccggggt gactttcccc tcccttagag ccacttctac cgcgatctct
     1861 agctggtcca tgtccgcctc gaactcggag agcacagtct gcgccctgcg ctcctcgatg
     1921 gccacctcct gcaaggattc catgatccag gctgcaaaca gcgccacggc gatcgttgcc
     1981 accgggataa accatctggg aattcctgaa acatctttct cccgccgcgg ccctcgccca
     2041 gcggaaggga tagtattacc cgccacctag atttctccct caaggtgtcc gatgttcgct
     2101 tttatttgac gccgacctgc tgagaacctg gaccggcaag aaatacgctc gacgctggca
     2161 gatggacgca aacgcttccc ttcttcagct atctctggag gagcgttccc ttcatctccc
     2221 ccgctgttcg gtagcccggc tgcctcacca acgactggct gcttcttgag gcccgtggct
     2281 ttgcgcgcac ctgccttgcg gcagggttgc ctttctcgca gcgcttcgag atgcgtctgc
     2341 gtcccctaat cttcgcatgc atcgccctcg cgtacaagga acccgcatgc catcgcagcc
     2401 gggctactcg aagacgacgg ttctcccgcc gtccaccaga atgcggtcct cgaggtgcca
     2461 gcggacggcg cgggcgagga ccctgcgttc gacctcgctt ccgaggcgca cgagatcttc
     2521 gaccgtgtcg cggtgcgtga cgtgggctac gtcctggtgg attatggggc cggcgtcgag
     2581 ctcatcggtg acgtagtggg cggtagcccc gatggtcttc actcccctct cgtgagccct
     2641 gcgatacggg tcagccccgg cgaaagcagg caggaaagag tggtggatgt tgacgatcct
     2701 gtggggatag gcgtcgacaa atcctggcga cagtatctgc atgtagcggg caagcacgac
     2761 gaaatcgacg ccatgctccg cgagcagatc caggatctcc gattcctgct cggccttggt
     2821 ctcccgcgtc acgggaaggt aatggaaggg gatgccgtat gccccgaccc ttgcggcgag
     2881 gtcacgatga ttggagacga cgagcggtat ctcagcctcg agctctcccg agtcccagcg
     2941 ccacaacaga tccatcagac aatggtcgta gcgggagacc agtatggcca tgtgcttcgg
     3001 ggtgtccgtg tacgacaccc gccagtgcat gccatgtttt cgggccagcg gcgcgaaggc
     3061 gccgccgaac tcgtcgcgcg ccaggttgaa gccttggccc tcgatctcca gccgcatgaa
     3121 gaacgtgcct ccggcttcgc catcgctcac gtactggtcg gcggagagga tgttgccgtc
     3181 gtgcatcgct atgaacgagg agaccgcggc aataaggccc ctacggtcag ggcaggagat
     3241 caggagtctg agattgtagc ccacgaggtc taatcctagt gaggcgaacg gggcgacgca
     3301 agatcagccc atcatcctca tgtagagagg catctgtgtg agaagctcgc ttctacgcgg
     3361 cacgatgacg cacacgttac aataccgacg ggatgcggcg cgcctgatct gttgcagaga
     3421 gctattgagc cgggtcacaa tcgcatccga gtactggtat gaagcgagga cacaactcca
     3481 gacaactccc acgagaagga ctcagcgggc cgcaggaggt agtccgcctc aagttgctcg
     3541 ggggcttcag ggtcactatc ggggaccgcg ccatcgcgga agccgggtgg cggctcagga
     3601 aggcagcgag cctggtgaaa ctgctcgcgc tcgcgccggg tcgccgtctc caccgcgagc
     3661 aggtcatgga tgcgctgtgg ccggacctcg ggcggcgggc ggcctccaac aacctgcgcc
     3721 agatcctcca cgtcgcccgc aaggcgctcg atccgaactc cgcctcccgc tgcctgatct
     3781 accgcgacgg tcaactactg ctgtgcccgg agggtcctat cagggtggac gtcgatgcct
     3841 tcgaggaagc cgcggccacg gcgcgacgcc tcggagagcc ggcgggctac cgtgcggcca
     3901 tagatctgta cgcaggggat cttctgcccg ccgaccgcta cgaagactgg gccgaggaac
     3961 gccgcagtga gcttcgcagg actttcatct ccctcctcgt cgaactggcg tcaatctacg
     4021 agcagcagag ggaatacggg ccctccatcg aaacactgga aagggctgtg gccggggaac
     4081 cactgcacga ggaggcgcga gtcgccctga tgcgggtcca cgccctcgcc ggtagtcctg
     4141 cggaggccct caagcagtac gagcggctcg agcacgctct gtatcgggag ctcggttcga
     4201 gaccagaggc ctcctcgcgc ctactgcgcg aagagatagc cgcagggcgc ttcccgccgc
     4261 gcgagacgag cacaacaagc gactcgttgt cggcaaaggc acgcacgcac aacctgcctg
     4321 ccgtccgtac gaagctcgtt ggccgcgagc gggagatgct cgagatcaaa cgcgagctcg
     4381 caatgacgcg cctcctgacg ctcacgggag taggaggttc gggcaagacg cggctggctc
     4441 tcgaggttgc cagggacctc accggggctt atccggatgg cgtatggctc gccgaactgg
     4501 cacctctctc cgagggtatg ctcgtgcctg gggcggtcgc ggaggcgctg gggatcaagg
     4561 agcgtgcagg ccagtcgctc gtcgatacgc tcgtcgaggc cgtgcgaccc agggagacgc
     4621 tactcgtcct cgacaactgc gagcacgtcg tcgagcaggc agcgcgcctg tccgaggtgc
     4681 ttctcgacgc gtgccctggt ctgcgcatcc tcgccaccag ccgtgaggcc cttggcgttg
     4741 caggcgaggt gagatgggtc gtccctgccc tcacgacgcc cggtctccgg cgttcctggt
     4801 cggtggaaga gctcgaggag tacgagtcgg cgcggctgtt tgcagagcgg gccaggcaga
     4861 gggatccctc tttcacatta acggctgcca gggcgcaacc ggtggcccag atctgcacgc
     4921 gcctcgaagg catcccgctc gccatcgaac tcgccgcagc gagggtaggt atgctgggaa
     4981 ccgcccagat ctcagaacgg ctcgaggatt cgatcgggct tcttaccacg actgtccata
     5041 ctgccgtccc gagacagagg acgctacgca gcgcgctgga ctggagccac gagctactct
     5101 ccgcggacga aaa
//
LOCUS       DATAIR010000360         8574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_300613, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000360 DATAIR010000000
VERSION     DATAIR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300613"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..563
                     /locus_tag="VHM69_08795"
     CDS             342..563
                     /locus_tag="VHM69_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX2740530.1"
                     /translation="MRVQIDRFEDNGWAVLLPYPDGRKSFDVPRELLPEEVSAGDIFE
                     IRFEYDEEETVRVAEENRRLLDELLGGEG"
     gene            560..2197
                     /gene="argS"
                     /locus_tag="VHM69_08800"
     CDS             560..2197
                     /gene="argS"
                     /locus_tag="VHM69_08800"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2740531.1"
                     /translation="MSFEERLADAVREAVQKTYGVDLDGVHVERPNDPSHGDFATNVA
                     LANARVLRRNPREVAENLVEALEAPFVREAEVAGPGFVNFRLSSEALLEEVGALLDEG
                     DRYGRREPSGAPVQIEYVSANPTGPLTVAHGRHAAYGDSLARVVAAAGRNVSREYYFN
                     DGGNQIRLFGESVAVRYARLFGEEWPVSDPDALYKGDYTEEIARDLVEAHGRRLLEIE
                     PDEALREITDFATRWSMDDIKRTLTRVGVHFDSFFNEKSLYESGAVKEIVQELEETGN
                     VYESEGALWLATSRFGDDKDRVIVKGDGSYTYVAPDIAYHKDKWERGFRTAVNVLGAD
                     HAGYGPRLRAGLVALGIPADFLDVELVRLVKLVREGEQVKFSKRAGNIVTFDELLDEV
                     GEDVARYFYVRSSHKTEMNFDLDLAIRQSDENPVFYVQYAHARIASIFERAGTSPDAL
                     GGIPVGDLAPEERLLVLELLDFPRVIQNAASRREVHPVPTYLETLATRFHQFYTVHRV
                     LVDDADVRARRLALCAATKTVLRSGLDLLGVEAPEKM"
     gene            2253..3116
                     /locus_tag="VHM69_08805"
     CDS             2253..3116
                     /locus_tag="VHM69_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX2740532.1"
                     /translation="MVNEDISKGYAGEIRFITYLSPGIPRDLFETLAEHVRRVLGCGR
                     VSLQVETRFSGPKKGVRDPFSAGEADVGFMCAPSYVWLRELRPPPVELLGVVPVFEDE
                     RNLGRPVYFCDVVVRGEGPIRSFAELEGGSWAYNDTCSLSGYYSLLEKLTQLAEDESY
                     FDEVFCSGSHLNSIEAVVSGVADAAAIDSNVLRIRLREVPALREQLRVIESWGPFPIQ
                     PVVVRSTLHPEVKEGLRTAFLSAQENQRTRRLLDRFDFSRFVAVSQEDYSPAVLAFES
                     HEDKGGVASCS"
     gene            3224..4048
                     /locus_tag="VHM69_08810"
     CDS             3224..4048
                     /locus_tag="VHM69_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2740533.1"
                     /translation="MSTQDRMVICQISDIHCGSPYFIPDLLERSILEVNDLDPTAVVV
                     SGDLTNAGYRQEFETAAEYIQRIQCERVMVIPGNHDSRNVGYIHFERLFGDRFPTMEF
                     EGAVMVAVDSSEPDLDDGRVGREHYGFIHETFAKAEDKLKIFVIHHHLIPIPGTGRER
                     NIIYDAGDVLELLADTEVDLVLSGHKHVPYSWKLENMFIVNAGTASTTRLRGNTRPCY
                     NVIEIEDGRVKVFRKYPFKDRELIVDFDSGTHQYLHYEEVQGDGRSVDRRERRTMS"
     gene            4055..5368
                     /locus_tag="VHM69_08815"
     CDS             4055..5368
                     /locus_tag="VHM69_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEX2740534.1"
                     /translation="MRALFLIDGEHYPPVVLDAMQSVRESLDAEGVAAAFLGGTEKLK
                     EGTDYGVPLVTGKDPVSAVKKALDEYEVDVVVDLSDEPVVGYRERMRIASLVLFAGAR
                     YLGSDFELRPPDLRQVSTKPSIAVIGTGKRVGKTAISGYLARLLASEGFDPGVVSMGR
                     GGPPQPEVIEGHKLEVGSDFLLEALERGAHAASDYYETAALSRVTTVGCRRCGGGLAG
                     EPFVSNVLEGAEIANGLDTRVTVFDGSGAAMPPVEVERRVLVAGAHQYPEYIVGFLGT
                     YRLLLSDLLLLTMSEEPMADRQKVDSLVSAVREVRPDLNVIPTVFRPRPVGEVEGLRV
                     GYVSTAPPAVLDKLARHLEDEYGCEVVATSGNLSDRKRLSADLDGMPRVEAYLTEIKA
                     AAVDVVTRRGSEEGKPVLYCDNDPVGEGLDSELLRLAREAVAREP"
     gene            5569..6567
                     /locus_tag="VHM69_08820"
     CDS             5569..6567
                     /locus_tag="VHM69_08820"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEX2740535.1"
                     /translation="MAAAAVEYAAVTERVALKVARLEGSGDSERAYTLATEAFREELE
                     KIPVSGRVTVLREQRLDERGRVRAPKSNPGIVSPDDTLGTGEPQADLAVEPIEGLQLL
                     VKGQEGSISAVAAAPRGTMLRTPDMYMSKLIVGPGARGSIDIDAPVSENIEAIAGAMG
                     RRPSEITVAVLDRDRHESLIDEIRKTGARIQIRPEGDLTPAIAVGMRGADLHAIIGIG
                     GAPEGILAAAVMKCLGGEMQARMWPTQRSQVEKLEEYGLTDPERKLTLDDLIRGTDVV
                     FAATGITPGETLNGVRYFRGGARTHTVVMSTEPRVVRFMDTIHALEPEAGRLGFQL"
     gene            6634..8118
                     /gene="rho"
                     /locus_tag="VHM69_08825"
     CDS             6634..8118
                     /gene="rho"
                     /locus_tag="VHM69_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX2740536.1"
                     /translation="MTELSTLERKRLSDLHQIASQLGIKQYRKYRKPELAEMIYKVAT
                     EQAASQQDNTANGDAAGVAAPPAANGGAAAPEKEKSEPVGTVDGAVEAPPEPPAPEHR
                     RQQRQEERRRPDRKQDRGGRGDEQVQSGILDVLPDGFGFLRTGGYSQSKGDVYVSTSQ
                     IKRFNLRRGDYITGQIRPAREGEKYPAMVRIETVNGQEPQKGRWRPNFDDLTPLYPME
                     RLRLEWKPNDIAPRVIDLVAPIGKGQRGMVVSPPKAGKTTILKQIAQSIAANYPETKL
                     FVVLADERPEEVTDWERSVEEAEVVASTFDQPADNHIAVAELVLERVKRLVEEGEDVV
                     VLLDGITRLSRAYNLAAPASGRILSGGVDSAALYPPKKFFGAARNIENGGSLTILATA
                     LVETGSRMDEVIFEEFKGTGNMELRLDRKLANRRIYPAINIEDSSTRKEELLMEPSEA
                     QRVWQVRSILNALDSAQKIELLIQKLKETRTNPEFLRELQRVRG"
     gene            8215..8430
                     /gene="rpmE"
                     /locus_tag="VHM69_08830"
     CDS             8215..8430
                     /gene="rpmE"
                     /locus_tag="VHM69_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2740537.1"
                     /translation="MKAGIHPEYTKTLVRCMCGNEFETRSTAGDTITVDLCSECHPFY
                     TGKQRIVDTGGRVQRFQNRRQASQNRQ"
BASE COUNT         1711 a         2541 c         2872 g         1450 t
ORIGIN      
        1 gtccgcttgg cctctccact gaggtgttgc aaagagcgga cgaactctgg tccttcggaa
       61 agatcacact ccctcacgcc ctcgccaggg tcgtcctcct cgaacagctc taccgcgccg
      121 taaagataca gcgaggcgag aagtatcact ggtagctccg ctttgctccg caggcatcag
      181 gtctcaggct tcagcaaggt ctctccggtc gaatctcaag ttcacaccac cgccaggctg
      241 gccttgcgcc ggcaggtagc gcttcgtaga ggatggagcg cggccaacag ccaaaaacct
      301 gcatcctgta acctgaagcc tgactgctaa aatacacata tatgcgcgtg cagattgaca
      361 ggttcgagga caatgggtgg gctgtgttgc tgccgtaccc tgatgggaga aagagcttcg
      421 acgtgccgcg cgagttgctg cctgaggaag tttccgccgg ggatattttc gagatccgct
      481 tcgagtacga cgaggaggag accgtgcgcg tcgcggagga gaacaggcgg ctactggacg
      541 agcttctcgg gggtgaagga tgagcttcga ggagcgtctt gcggacgccg tgcgggaggc
      601 cgttcagaag acgtacgggg ttgatctcga cggcgttcac gtcgagcggc cgaacgaccc
      661 gagccacggt gacttcgcga ccaacgtcgc cctggccaac gccagggtac tgcggcggaa
      721 cccgagggag gtcgcggaga acctggtgga ggctctggag gcgccgttcg tgcgggaggc
      781 cgaggtagcc ggtccgggat tcgtcaactt caggctgtct tccgaggctc tgctggaaga
      841 ggtcggggca ttgcttgacg agggggaccg gtatggacgc agagagcctt ccggggctcc
      901 tgtccagatc gagtacgtga gcgcgaaccc aacgggtccg ctcaccgtgg cgcacggacg
      961 gcacgcggcc tacggtgact cgctcgcgcg tgtggtcgcg gcggctggaa ggaatgtctc
     1021 gcgagagtac tacttcaacg acggggggaa ccagatacgg ctcttcggtg agtcggtggc
     1081 cgtgcgatac gcgaggctct tcggtgagga gtggcccgtc tctgaccctg atgcgctgta
     1141 caagggcgac tatacggagg agatcgccag ggacctcgta gaggcgcatg gccgtcgcct
     1201 tctggagatc gaaccggatg aggccttgcg ggagatcaca gacttcgcga cccgctggag
     1261 catggacgac atcaagcgca ctctgacccg agttggcgtc cacttcgaca gcttcttcaa
     1321 cgagaagagc ctatacgagt cgggtgctgt gaaggagata gtccaggagc tagaggagac
     1381 aggcaacgta tacgagagcg agggtgccct gtggctggcg acatcgaggt tcggtgacga
     1441 caaggacagg gtgatcgtaa agggcgacgg gtcctatacc tacgtggcac cggatatcgc
     1501 ctatcacaag gacaagtggg agaggggctt caggacggcg gtgaacgtcc tgggggccga
     1561 ccacgccggg tacgggccga ggcttcgggc cggactcgtg gcgctcggga tcccggccga
     1621 cttcctcgac gtggaactgg tgcgcctagt caagctcgtg cgggaaggcg agcaggtcaa
     1681 gttcagcaag cgtgcaggca acatcgtgac cttcgacgag ttactggacg aggtcgggga
     1741 ggacgtggcg cgttacttct acgtgcgatc ctcccacaag acggagatga acttcgacct
     1801 cgaccttgcg atccgccagt ccgacgaaaa cccggtcttc tacgtacagt acgcccacgc
     1861 ccgcatcgcc agcatcttcg agcgggcagg cacgagcccc gacgcattag gcggcatccc
     1921 cgtaggggat ctcgcccccg aggagcgatt actcgtgctg gagctgctcg acttcccgcg
     1981 tgtgatccag aacgccgcct ccagacgcga ggtgcatcct gtcccgacgt acctcgaaac
     2041 cctagccacg cgattccacc agttctatac agtgcaccgc gtcctggtag acgacgccga
     2101 cgtacgcgcc cgccgcctcg cactgtgcgc agcgaccaag accgtacttc gcagcgggct
     2161 cgacctgctt ggagtagaag ctccggagaa gatgtagaga gcgtattgcc gtagggtggc
     2221 cgatgggtcc ataatggcac ccagaggtag agatggtgaa tgaggacatc agtaagggat
     2281 acgccggaga gatccgcttc atcacctatc tgtccccagg catcccacga gatctcttcg
     2341 agacgctggc tgaacacgta cgccgggttc tcgggtgcgg gcgggtctcg ctgcaggtag
     2401 agacgaggtt ctcaggacca aagaaggggg tgcgagatcc cttctcggcg ggagaggccg
     2461 acgtgggatt catgtgcgcg ccatcgtacg tctggctgcg ggagcttagg ccgccgcccg
     2521 tggagttgct cggcgtggta ccagtgttcg aggacgaaag gaaccttggt agacccgtct
     2581 acttctgcga cgtcgtggtg cgcggggaag gtccgatccg atccttcgcc gagctcgaag
     2641 gaggctcgtg ggcgtacaac gacacctgtt cgttgagcgg ctactacagt ctgctcgaga
     2701 agctcaccca gttggccgag gacgagagtt atttcgacga agtgttctgc tcgggctccc
     2761 acctgaactc catagaggct gtcgtcagcg gcgtggcgga cgcggcggcc atagattcca
     2821 acgtcttgag gatacgactc agagaggtgc ccgccctgcg cgagcagttg cgcgtgatcg
     2881 agtcctgggg accgttcccg atacagcccg tggtggtccg ctccacgctg catcctgagg
     2941 tgaaagaagg actgcgcacc gccttccttt cggcgcaaga gaaccagcgc acgcggcgac
     3001 ttctggacag gttcgacttc agccggttcg ttgccgtaag ccaggaagac tactccccag
     3061 cagtactcgc cttcgagtct cacgaagaca aaggaggcgt cgctagctgc tcctgatcca
     3121 gcgtccagtc gggaggcggt gtgaggtgag cgggcacggt ggcgccgaag gttactctgt
     3181 tatacttttc gaggtatttc gtggcctatg gaatcggggg taaatgagca cgcaggacag
     3241 aatggtcatc tgtcagatca gcgacataca ctgcggcagc ccttacttca tacccgactt
     3301 gctggagcgt tcgatcctgg aggtaaacga cctcgacccg accgcggtcg tggtctctgg
     3361 tgacctgacg aacgccggtt accgacagga gttcgagacg gctgcggagt acatccagcg
     3421 catccagtgc gagcgcgtca tggtgatccc tggcaaccac gattcgcgga acgtcggcta
     3481 cattcacttc gagcggctct tcggagaccg gttcccgacc atggagttcg agggagccgt
     3541 tatggtcgcc gtggactctt ccgagcctga cctggacgac gggcgggttg ggcgtgagca
     3601 ctacgggttc atccacgaga ccttcgcgaa ggcagaagac aagctcaaga tcttcgtgat
     3661 ccaccaccac ctgataccga tccctggaac gggccgcgag aggaacatca tctacgacgc
     3721 cggcgacgtt ctggagttac tggccgacac agaggtcgat ttggtcctat cgggccacaa
     3781 acacgtgcct tactcgtgga agctcgagaa catgttcatc gtcaacgctg ggaccgcctc
     3841 gacaacccgc ctcaggggca acacgcgccc ctgctacaac gtcatagaga tcgaggatgg
     3901 gcgagtaaag gtcttccgca agtacccgtt caaggaccgg gagctgatcg ttgacttcga
     3961 ctcagggacc caccagtacc tgcattacga ggaggtccag ggcgacggca ggtccgtgga
     4021 ccgccgcgag cggcgaacga tgagctagag cagcgtgcgg gcgctgttcc tgatcgacgg
     4081 cgagcactat cctcccgtcg tcctcgacgc gatgcaaagc gtgcgggagt cactcgacgc
     4141 cgaaggcgtg gcagcggcgt tccttggcgg cacggagaag ctcaaggaag gcacggacta
     4201 cggcgttccg ctggttaccg gtaaggaccc agtctcagca gtaaagaagg cgctcgatga
     4261 gtacgaggtt gacgtggtgg tcgacctctc agacgagccc gtggtcggtt acagggagcg
     4321 gatgaggatc gcctccctcg tgctcttcgc cggggcgcgg tacctcggca gcgacttcga
     4381 gctgaggccg ccggatctgc gccaggtctc gaccaagccg tcgatcgccg tgatcggaac
     4441 aggcaagagg gttgggaaga cggcgatctc gggctacctc gcacgcctgc tcgccagcga
     4501 aggcttcgat cccggcgtgg tctcgatggg tcgcggaggt cctccgcaac ccgaggtcat
     4561 cgagggtcac aagcttgagg tcggcagcga ctttctgcta gaggccctcg aacgtggggc
     4621 gcacgcggcg agcgactact acgagaccgc ggcccttagc agggtcacca cagtcggatg
     4681 ccgcaggtgc ggcggcggtc tcgcggggga gcccttcgtc tccaacgtcc tcgagggtgc
     4741 tgagatcgcc aacggcctcg acacccgcgt taccgtcttc gacggctcgg gggcggccat
     4801 gcctcccgtc gaggtcgaga ggcgcgtgct cgtcgcgggg gcccaccaat accccgagta
     4861 catcgttggc ttcctcggca cctaccggct gctcctctcc gacctcctgc tcctcacgat
     4921 gagcgaagag cctatggcag accgtcagaa ggtggatagt ctcgtaagtg cggtgcggga
     4981 agtacgtccc gatctgaacg tgatcccgac ggtcttcagg ccgcgtccgg tcggggaggt
     5041 cgaaggactg cgtgtcggct acgtctccac ggcgccgccc gccgtgctcg acaagctcgc
     5101 ccgtcacctg gaggatgaat acggttgcga ggtagtggcc acttccggga acctttctga
     5161 caggaagcgc ctgagcgcgg acctcgacgg gatgccgagg gtcgaggcgt acctgacgga
     5221 gataaaggcc gctgcggtgg acgtcgttac gcgccgcggt tcggaagagg ggaagccggt
     5281 actctactgc gacaacgatc ctgtcggcga gggtctcgac tctgagcttc tcaggctcgc
     5341 gcgggaggct gtggcccggg agccgtgagc gggtagtcgc cgtccggtgg ctggtgtata
     5401 atcatgctgg aaggtacact ggtccctcac ctctgaaggc aaggggccgt tccgcaccgt
     5461 ttcgacgcag acccagcctg ggagcactcc gtggcgcgcg gtctatcttg ttggacatgg
     5521 gaggtctggt gagagcccat taacgttacc gatccccgct cggggtccat agctgcggct
     5581 gcggtcgagt atgccgcagt aaccgagcgg gtcgcgctga aggtggcacg tctcgaaggc
     5641 tctggggact cggagcgggc ttacacgcta gctaccgagg cgtttcgtga ggagcttgag
     5701 aagattccgg tctccggcag ggtgactgta ctgcgcgagc agcgcctgga cgagagaggg
     5761 cgcgtcaggg cgcccaagag caatcccggg atagtgagcc cggacgacac gctcggaacc
     5821 ggggagcccc aggctgacct cgcagtcgag ccgatcgagg gcttgcagtt gctcgtaaag
     5881 gggcaggagg gttctatctc ggcggtggcg gcggccccga gggggaccat gctcaggacg
     5941 ccagacatgt acatgagcaa attgatcgtc ggccctggag cgaggggaag catagatatt
     6001 gacgctcccg tctccgagaa catagaggcc atagccgggg cgatgggccg caggccgtcg
     6061 gagatcaccg tcgccgtgct cgaccgcgac aggcacgaga gcctcataga tgagatccgc
     6121 aagaccgggg cgcgcataca gatccggccc gagggcgacc tgacgccggc gatcgcggta
     6181 gggatgcggg gcgctgacct gcacgcgatc atcggaattg gcggtgcgcc cgaaggcatc
     6241 ctcgcagcgg cggtcatgaa gtgcctcggc ggggagatgc aggccaggat gtggccgacg
     6301 cagcgctcgc aggtcgagaa gctagaagag tacggcctca cagacccgga gcggaagctc
     6361 acgctggacg acctgatccg gggtacagac gtggtcttcg ccgcgaccgg tataacgcca
     6421 ggcgagaccc ttaacggggt taggtacttc aggggcggcg cgcgaacgca tacagtcgtc
     6481 atgtcaaccg agcctcgtgt ggtaagattc atggatacca tccacgccct agagcccgaa
     6541 gccgggcgac tgggcttcca gctttgacaa actcacgaag aaatcagatg aaagacatcc
     6601 ccgcatcgca tacaggaaag gtggagtagc aacatgaccg aattatcgac cctggagcgc
     6661 aagcgcctga gcgacctgca ccagatcgcc tcgcagctcg gaatcaagca gtaccgcaag
     6721 taccgcaagc cggagctggc ggagatgatc tacaaggtcg ccaccgagca ggccgcgagc
     6781 cagcaggaca atacggctaa cggagatgcc gccggtgtcg ccgcgccgcc cgcggcgaac
     6841 ggcggggccg cggcgcccga gaaggagaag agcgagcccg tcggtaccgt cgacggcgcc
     6901 gtcgaggcgc cgccagagcc cccggcacct gagcatcgca ggcagcagag gcaggaggag
     6961 cggaggcgcc ccgaccgcaa gcaggaccgt gggggccgcg gcgacgagca ggtgcagagt
     7021 ggcatcctcg acgtattgcc cgacggcttc gggttcctca ggacgggggg atacagccag
     7081 agcaagggcg acgtatacgt ctcgaccagc cagatcaaac gcttcaacct caggcgtggg
     7141 gactacatta cgggccagat ccgtcctgcc cgcgagggcg agaagtatcc ggcgatggta
     7201 cgtatcgaga cggtaaacgg acaggagccg cagaagggcc gctggcgccc gaacttcgac
     7261 gacctgactc cgctttaccc gatggagcgg ctgcgcctgg agtggaagcc gaatgacatc
     7321 gccccgcgcg tcatagacct cgtcgcgccc atcggcaaag gtcagcgcgg catggtcgtc
     7381 tcgccgccga aggccggtaa gacgacgatc ctcaagcaga tagctcagtc catcgcggcg
     7441 aactaccccg agaccaagct cttcgtggtg ctcgcagacg agcgccccga ggaggtaacg
     7501 gactgggagc gcagcgtcga ggaggccgag gtcgtcgcct ctaccttcga ccagcccgcg
     7561 gacaaccaca tagctgtcgc cgagctggtc ctagagcgtg tcaagcgcct ggtcgaggag
     7621 ggcgaggacg tggtcgtcct gctcgacggt ataaccaggc tctcgcgtgc atataacctg
     7681 gctgctcctg cttcagggcg aatcctctcc ggtggcgtcg attcagcggc gctgtatccg
     7741 cccaagaagt tcttcggggc tgcgcgcaac atcgagaatg gtggctcgct gaccatcctc
     7801 gcgacggcgc tcgtcgagac cgggagcagg atggacgagg tcatcttcga agagttcaag
     7861 ggcaccggca acatggagct cagactggat cggaagctgg cgaaccgcag gatctacccg
     7921 gccatcaaca tcgaggactc atcgacgcgc aaggaggagc tgctcatgga gccttccgag
     7981 gcacagcggg tctggcaggt ccgcagtatc ctgaacgccc tcgacagcgc gcagaagata
     8041 gagctcctga ttcagaagct caaggagacc cgcacgaacc ccgagttcct gcgcgaactc
     8101 cagcgcgtcc gcggttaacc gatagatcta gacgggcgtg gacaccgcgc ccgaccttcc
     8161 ctttacaagg gtaataaact atatgagacg gtaattgaaa gtcggaggtt tcagatgaag
     8221 gctggcatac atcccgagta cacgaagacg ctcgtgcgct gcatgtgcgg caacgagttc
     8281 gagacgcgct cgacggcggg ggacaccata acggttgacc tctgctccga gtgccacccg
     8341 ttctacactg gcaagcagcg catcgtcgac accggcggtc gggtgcagcg gttccagaac
     8401 cgccgccagg ccagccagaa caggcagtag agcaaaagag catgagggtc ggcggacagg
     8461 ccatcatgga gggcgtattg atgcgctctc ccaatttctg gggcatggcc gtccgcacgc
     8521 cctcggggga tgtggacctg cgggcggagc ggttccgttc cgttacgcgc cgca
//
LOCUS       DATAIR010000361         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_333514, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000361 DATAIR010000000
VERSION     DATAIR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333514"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VHM69_08835"
     CDS             complement(<1..603)
                     /locus_tag="VHM69_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2740538.1"
                     /translation="MRKLIVSTFLTLDGVMQAPGGPEEDDSGGFAHGGWSVNYWDEHV
                     GRFMDEAMSKPFDLVLGRKTYDIFAAYWPHAPEETGAKPLNDATKYVASRSHPALEWS
                     NSVLIEGDVAEGIAALKQEDGPELQVHGSGNLIQTLLRHNLVDQYRLLIFPLVIGSGK
                     RLFSEGTIPSGLRLVDSKVSTTGVVMGTYEPAGEIVTGSFA"
     gene            complement(811..1386)
                     /locus_tag="VHM69_08840"
     CDS             complement(811..1386)
                     /locus_tag="VHM69_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2740539.1"
                     /translation="MTDRGGPPRPGATKNAALAEVSLLLAVAGVALVVGGGLGSEEAS
                     VGGDLLVCVSAFSWGAYTVLSLPVLRRIDPLVVGGWTMLLGGLAVLPLAFTGFPNLSG
                     SLSSVEWGSVGATAWAAAAYSTVLCSSFAIAAYQASVARRGANRVLVYLYLQTLVGLL
                     ASVLLLGEGLGAAKVVGAAVILLGVYLARRA"
     gene            1673..2635
                     /locus_tag="VHM69_08845"
     CDS             1673..2635
                     /locus_tag="VHM69_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2740540.1"
                     /translation="MQASPVFLDRDATIVRALELIAEAGRSGARLVVFPEAFIPTYPD
                     WVWRIPPGQHRMLADTYAELLEQSVEIPGPVTEELSQAARQAGVYLVMGLNERNAQAS
                     NASLYNTLLYIDPQGNLLGKHQKLVPTAPERMVWAQGDGSTLEVYETSLGRLSGLICW
                     ENYMPLARYSLYAWGTQIYLAPTWDSGEPWLSTLRHIAKEGRVYVVGCAIAMRKEEIP
                     EQFEFKAKYYSEVGEWINKGGSAIVSPDGKLIAGPLNEEEGIIYAEVDPKQMRGPKWN
                     LDVAGHYARPDVFQLTVSKEDRPIINVAETPEQNLQEATEVLED"
     gene            2798..3037
                     /locus_tag="VHM69_08850"
     CDS             2798..3037
                     /locus_tag="VHM69_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2740541.1"
                     /translation="MRLLYIVTKGMNDPTLASVPLHIAANGSLEVGQDVSVVLAGDAT
                     DLVIGDNAQRVEGVGVPPARELLSKLKEHEVPLHI"
     gene            3293..3547
                     /locus_tag="VHM69_08855"
     CDS             3293..3547
                     /locus_tag="VHM69_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEX2740542.1"
                     /translation="MEFVVLILVVIVVLFLLTRGGRFLGRLIINGIVGLVLLFLTNLV
                     LADDIPINVLTVIICAIGGIMGWLIILVLHLLGVAFYIPA"
     gene            complement(3695..>4102)
                     /locus_tag="VHM69_08860"
     CDS             complement(3695..>4102)
                     /locus_tag="VHM69_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX2740543.1"
                     /translation="MSVRIASCSCGQLQAEVRGEPIGVSVCHCYACQRRTGSVFSAQA
                     SFPRENVTIKGNGTEFVRVGDEGGRFKFTFCPECGSTVFYVEEGDEQEIAIPVGAFAD
                     SRFPAPTVSVYEKRRHSWVTLPDDVEHWRKDPE"
BASE COUNT          838 a         1282 c         1227 g          755 t
ORIGIN      
        1 cgcgaacgat cctgtgacga tctcgcccgc cggctcgtac gtgcccatca cgactccagt
       61 ggtggagacc ttgctgtcga cgagcctcag cccggagggg atggtgcctt ccgagaacag
      121 gcgtttgccc gacccgatga ctagggggaa gatcaacaaa cggtattggt cgacaaggtt
      181 gtggcgcaga agcgtctgga tcaggttccc gctgccatgc acctgcagct ctggcccgtc
      241 ttcctgtttg agtgccgcga tgccttcggc gacgtcgccc tcgatcagga ccgagttgct
      301 ccactccagc gcgggatggc tccgcgacgc gacgtacttg gtggcgtcgt tcaacggctt
      361 ggcgccggtc tcctccgggg cgtgcggcca gtatgctgcg aagatgtcgt aggtcttgcg
      421 tccgagcacg aggtcgaacg gcttgctcat cgcctcatcc atgaaccgcc ccacgtgctc
      481 gtcccagtag ttcaccgacc agccaccgtg cgcgaacccg ccgctgtcgt cctcttcggg
      541 tccgcccgga gcctgcatga ccccgtcgag ggtcaggaag gtgctcacga tcaactttct
      601 catctctcgt gctccttact ctcgtttctg tcccatacac ccacgactaa cagtacacac
      661 cgacgacgaa cgggccaccc caaatctagg cttaactact taccttcaac gcaggcggat
      721 gaatagcaga gacctcgcct gtgtgtccgg ctcgtacggg ccaaccgacg acggcggaac
      781 ggacccggtc ccgaccagag gcttccgagg ctaggcgcgc cgcgccaggt agacgccgag
      841 caagatgacg gccgccccca ctaccttggc tgcccccaga ccctctccta gcagcaggac
      901 gctggccagc agccccacca gtgtttgcag gtacaggtag acgaggaccc tgttggcgcc
      961 cctccgggcg acgctcgcct ggtacgcggc tatggcgaag ctgctgcaca gcaccgtcga
     1021 gtaggcggcg gccgcccaag ccgttgcgcc caccgagccc cactccaccg aggagagcga
     1081 ccccgagagg ttaggaaagc cggtgaaagc cagggggagg accgcgagcc cgccgagcag
     1141 catcgtccag ccgcccacca ccagggggtc gatgcggcgc aggacgggca gggagagcac
     1201 cgtgtaggcg ccccaggaga aggcactcac gcacacgagc aggtcgccac ccacggacgc
     1261 ctcttccgag cccaggcccc cgccaacgac gagagccacg cccgcgacgg ccaggagcag
     1321 tgagacctcg gcgagcgccg cgttcttcgt cgcgccgggc cgcggaggcc ccccccgatc
     1381 cgtcaacctc gtcgcgggat tggggtcgtg catcgcatgc gcgcaatctt agcgccggaa
     1441 agatccgacc gcctacgcgg cggccggcta gagctccggc ctgtcatcgg caccctgcaa
     1501 gcgctcagcg ctatgcgaac ttgcgagaac tccgtaaagg cgaagttccg agagaagctc
     1561 aattcttcga cagatgcacc gagattaatt aggtgccatc attgcggtag agaaggagtg
     1621 atctgagaat tggaaggagc gcacgatcgc atcgaccttc aaaatagccg ctgtgcaagc
     1681 gtcacccgtg ttcctcgacc gcgatgccac catcgtcagg gccctggagc tgatcgcgga
     1741 agcgggaaga agcggggcac gcctggtagt cttccctgag gccttcatcc cgacctaccc
     1801 ggattgggta tggcgcatac cacctggcca acaccggatg ctcgccgaca cgtacgcgga
     1861 gttgctcgag caatcggtcg agattcccgg cccggtcacc gaggagcttt cccaagcggc
     1921 aaggcaggcc ggcgtctacc tggtgatggg cctcaacgag cgcaacgcgc aggccagcaa
     1981 cgcgagcctg tacaacactc tgctctacat cgatccccag ggcaacctgt taggcaagca
     2041 ccagaagctc gtacctacgg cgcccgagcg catggtatgg gcacagggcg acggcagcac
     2101 actcgaggtc tacgaaacct ctttgggcag gctaagcggc ctgatctgct gggaaaatta
     2161 catgccgctg gctcgctatt cgctctatgc ctggggcacc cagatctacc tggctcccac
     2221 gtgggactct ggcgaaccct ggctgtccac cctcaggcac atcgccaagg aaggacgtgt
     2281 gtacgtggtt ggctgcgcga tcgccatgcg caaggaagaa atcccagagc agttcgagtt
     2341 caaggcgaag tattactccg aggtcgggga atggatcaac aagggaggca gcgccatcgt
     2401 gagccccgac ggcaagctca tcgccgggcc gctcaatgaa gaagaaggca tcatctacgc
     2461 cgaggttgac ccgaagcaga tgcgcggacc caagtggaac ctggatgtgg ccggccacta
     2521 cgcgcggccg gatgtgttcc agctcaccgt gagcaaagaa gaccgtccga ttatcaacgt
     2581 tgccgaaaca ccggagcaga acttgcaaga agccacggaa gtgctggagg actagacctt
     2641 cctgtgcatc acttcctcaa cgtagttctg tgaatgaacc gatagcgtcc tgcttacaat
     2701 agccaaatag caacagcaag aatcattcaa ttaggggatg cgctacctga cttttggagc
     2761 gtaggctgaa tccggatacg caaaagagga ggacaagatg aggttactct acatagtcac
     2821 taagggaatg aatgacccga ccttagcctc cgtcccgttg cacatcgcgg ccaacggctc
     2881 gctggaggtc ggtcaggacg tatccgtggt cctcgcaggg gatgcgacgg atttggtgat
     2941 cggagacaac gcccaacgcg tggagggtgt cggggtgcct cctgcgcgag agctgctgag
     3001 caagctgaaa gagcacgagg ttcctttgca catctgaaag gcttgcgcag tcgcccgtgg
     3061 ggcgacggag gagagcgcgg cggccgtcaa cggcagatgg acaaccccac cggatgtggc
     3121 gaggctcgcg gcggaggccg acaggatact gaccttttag gtgcgccagt gcgaactttg
     3181 gagaagactc cttctgcacc tctggtaaac aagtgtaaga gggaagtggt gcccagactt
     3241 cgtcttggga gctgcgcgta acggactagc gtctcatata caatggcgcc ttgtggaatt
     3301 tgtcgtactc atattggtcg taatagtggt cttgttcctg ctgacgcgag gcgggcgttt
     3361 tctgggtcgt ctcatcatca atgggatcgt aggtctggtt ctcctgtttc tgaccaacct
     3421 cgttctcgcc gacgacatac ccatcaacgt gcttaccgtg atcatctgcg cgatcggtgg
     3481 gattatggga tggctcatca tcctggtact ccaccttttg ggcgtagctt tttacattcc
     3541 tgcttaacag tgcccttggc ttctccaccc tcggctacta gctactccat ctcagcctca
     3601 ataggcggga cctccggctc gagcaccttg atgagggtgt agcgcccttc cgtttccttg
     3661 cacgtcagcg tcttcgaaga ccgatggtgc gggactattc cggatcctta cgccagtgct
     3721 cgacatcgtc gggaagagtg acccaggaat gcctgcgctt ctcgtagacc gagaccgtcg
     3781 gggccggaaa gcgcgagtcc gcgaaggccc ccacgggaat ggcgatctct tgttcatccc
     3841 cttcctcgac gtagaaaacc gtcgacccgc attcagggca gaaggtgaac ttgaatcttc
     3901 cgccttcgtc tcccacccga acgaactccg tgccatttcc ctttatggtc acgttttcgc
     3961 ggggaaagct cgcctgcgcg ctaaatacgc tgccggtcct gcgttggcaa gcgtagcagt
     4021 ggcagacgga gacaccgatc ggttctccgc ggacctccgc ctgaagctga ccacaactac
     4081 aagatgcgat gcgaactgac at
//
LOCUS       DATAIR010000362         3688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_195261, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000362 DATAIR010000000
VERSION     DATAIR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195261"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="VHM69_08865"
     CDS             <1..1798
                     /locus_tag="VHM69_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2740544.1"
                     /translation="KDAEGGPLYWQGVIFDMTERKKAEEELRKSQASLALAQRMAHLG
                     SWEWDLITGEVDWSDEVFRIYGFEPRSFVPTFERLLDIVHPEDRKTLRKAMEGALEEG
                     LPYDYEHRIVKPDGEVRVVHRRAEVVRAEDGEPIRMVGTVHDVTERKALEEQLQHQAL
                     HDPLTGLPNRTLFADRLRQILARARRRESETSVLFMDLDNFKVINDSLGHKAGDRLLV
                     SASKRISALLRPEDTVARLGGDEFIFLLEDTGTQNAVRVAERILKRMQEPFSLGGRRL
                     FVTASIGVATGGADAGYAADLLRNADLAMYKAKHSGKARYAVFEEAMNEKAIDRLEME
                     HGLRRALDRGELKVYYQPQMLLDTDADSAPGSREPRIIGMEALVRWEHPERGLLLPGE
                     FVPLAEETGLIVPMGTMVLEEACRRTREWRERFSGDPPLSVYVNLSAKQFREPDLTQT
                     VSRVLKETGLDPTCLHLEITESTAMSDAPATVSTLEELTSLGVRLVIDDFGTGYSSLS
                     YLERFPVDYVKIDRSFVGRLDSESGATALVSGMIGLAHALGIRVVAEGVENAEQLERV
                     HSMGCDLAQGRYFADPLPGEALGTLLESKSLK"
     gene            1892..2707
                     /locus_tag="VHM69_08870"
     CDS             1892..2707
                     /locus_tag="VHM69_08870"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX2740545.1"
                     /translation="MANALEALWTAFAEFAPRLLGAGLVLVATFLAARFLQRLGYRLL
                     QRIGLDALFERTGAAASLQQVGFGRSPSVVFGYVLFWGVLLAGVAAALSILGLASLQR
                     NMDLIVALAGKAVVAVLILAGGLAAAGWLSNLAAQQAENAGLMGGELFKRVIFAVVLT
                     IAALVAAAQVGIEVSILVLLALVLFGTVGIVAALVLGLGLAPLSGNIAAGRYVHGNGI
                     RPGDEISVNGVQGTVEELGLASVTLRSEDGRIHHVPNRILLEGIVSKTSQRDE"
     gene            complement(2732..3172)
                     /locus_tag="VHM69_08875"
     CDS             complement(2732..3172)
                     /locus_tag="VHM69_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2740546.1"
                     /translation="MTEKEREQFLAQPRVGVLSVASDNNRPPLTVPIWYGYEPGGNIT
                     FFTGTQGRKARKTELIRRAGVLSLSVQREEFPYKYVTVEGTVVGIYRPPSAGQMLAVA
                     RRYLPEEAASGFVEAELGRPSGELVLFTVRPDRWLTADFSDEAG"
     gene            complement(3227..3616)
                     /locus_tag="VHM69_08880"
     CDS             complement(3227..3616)
                     /locus_tag="VHM69_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740547.1"
                     /translation="MNAGPEASSSRAHEESEARIIDVADLAHSETAYEFEGHHHGDTN
                     VSFIVIDAPQGRGPKLHKHPYEEVFVVQEGEATFTAGDDVIEARGGQVVVVPAGVPHK
                     FVNSGAGRLRQVDIHASERFITEWLED"
BASE COUNT          646 a         1147 c         1202 g          693 t
ORIGIN      
        1 caaggacgcg gagggcgggc ctctttactg gcagggcgtc atcttcgaca tgaccgagcg
       61 caagaaggcc gaagaggagc tgcgcaagag ccaggcgagt ctggctcttg cgcagcgcat
      121 ggcgcacctc ggaagttggg agtgggacct gataacgggc gaggtcgact ggtccgacga
      181 ggtcttccgc atctacggct tcgaaccgcg gtcttttgtc cccaccttcg agaggctcct
      241 cgatattgtg catcccgaag acaggaagac acttcggaag gcgatggaag gcgccctcga
      301 ggagggcctt ccctacgact acgagcaccg catagtcaag cctgacggcg aggtcagggt
      361 ggttcaccgt cgggccgagg ttgtgcgcgc ggaggacggc gagccgatca ggatggtcgg
      421 caccgtccac gacgtgaccg agcgcaaggc cctggaggag caactccagc atcaggcgct
      481 gcatgatccc ctgacaggtc tgcccaacag gacgctgttc gccgaccgcc tccgccagat
      541 cctcgcccgc gccagacgca gggagagcga gacctcggtc ctgttcatgg acctggacaa
      601 cttcaaggtc ataaacgact cgctcgggca caaggccggc gacaggctgc tcgtttcggc
      661 gagcaagcgg ataagtgcgc tgctaaggcc ggaggacacc gtcgcgcgcc tcggggggga
      721 cgagttcatt ttcttgctcg aggacactgg cacgcagaac gctgttcgcg tcgcggaacg
      781 tatcctgaag cgaatgcagg agcctttctc cctcggcggg cgcaggctct tcgtcacggc
      841 cagtatcggc gtcgccaccg gaggcgccga cgcaggatac gccgccgacc tcttgcgcaa
      901 cgcggacctt gcgatgtaca aggccaagca ttccggcaag gcgcgctacg ccgtcttcga
      961 ggaggcgatg aacgagaagg ccatcgaccg tctcgagatg gagcacggct tgagaagggc
     1021 cctcgacagg ggcgagctga aggtctatta ccagccccag atgctcctgg acaccgacgc
     1081 ggacagcgcc ccgggatcca gggagccgcg gatcataggc atggaagcgc tggtgcgctg
     1141 ggaacaccct gagcgcggcc tgctgctccc gggagagttc gtcccgctcg ccgaggagac
     1201 cgggctcata gtccccatgg ggacgatggt gctggaggag gcgtgccgcc ggaccagaga
     1261 atggcgggaa cgtttctccg gagacccacc cctatccgtc tacgtaaacc tctcggcgaa
     1321 gcaattcagg gagcccgacc tcacccagac cgtctccagg gtcttgaagg agacgggcct
     1381 cgatcctact tgcctgcacc tggagatcac cgagagcacg gccatgagcg acgctccagc
     1441 gacggtctcg acgctggagg aactgacatc acttggcgta cgactcgtca ttgacgactt
     1501 cggcacgggg tactcttcgc tctcctacct tgagaggttc cctgtcgact acgtcaagat
     1561 agaccgctcg ttcgtgggcc gtctcgactc cgagtcgggg gctacggcgc tggtgtcagg
     1621 tatgatcggc ctggcacacg cactgggtat cagggtcgtc gccgaagggg tggagaatgc
     1681 tgaacaactc gaaagggtgc attctatggg atgcgacctt gcccagggac gctacttcgc
     1741 agaccccctc cccggcgagg cacttgggac gctcctcgag agcaagagct tgaagtagcc
     1801 tcatccgaac cggagtgacc ctggtgccag ggtaagctag tatgtggtct atcccgggga
     1861 gtagggtcag cacgcgcgga ggagttcatc gatggcgaac gctctggagg cattgtggac
     1921 cgctttcgcc gagttcgcac ccaggctgct cggcgccggt ctggttctcg tggccacgtt
     1981 cctggcggcg cgttttctgc aacgcctggg atacaggcta ttgcagagga tagggctcga
     2041 tgccctcttc gagaggacgg gggcggctgc atctttgcag caggtgggct tcgggagaag
     2101 cccttcggtc gtgttcgggt atgtgctctt ctggggggtg ctcctggccg gggttgcggc
     2161 ggccctgagc atccttggcc tggcttctct gcagcgcaac atggacctca tagtcgccct
     2221 cgcggggaaa gcggtggtcg cggtcctgat cctggccgga ggcctcgcgg ccgcgggatg
     2281 gctctctaac ctcgcagccc aacaggccga aaacgccggc ctcatgggtg gggagctctt
     2341 caagcgcgtg atcttcgccg tcgtcctgac gatcgcggcc cttgtggccg cggcgcaggt
     2401 aggaatcgag gtctcgatcc tcgtcttgtt ggccctggta ctcttcggga cggtgggaat
     2461 cgtggcggcg ctcgtcctcg gcctcgggtt ggctcccctc tcggggaata tcgcggcggg
     2521 ccgctacgta cacggaaacg ggatacgtcc tggggatgag atctccgtca acggtgtgca
     2581 ggggacggtc gaggaacttg gcctcgcctc ggtcaccttg aggtcagagg acggacggat
     2641 tcatcacgta ccgaaccgta tattgctcga agggatcgtg agcaagacct cacaacgaga
     2701 cgagtagagc gtacaccctt atgtgctgtc cttatccggc ctcgtcgcta aagtctgcgg
     2761 tgagccagcg gtcaggacgg acggtgaaca gcacgagctc gcccgagggt cttccgagct
     2821 ccgcttcgac gaatccgctt gccgcttcct caggcaggta ccggcgggcg acggcgagca
     2881 tctgcccggc ggacggcggt cggtagatcc cgacgacggt gccctcgacg gtgacgtatt
     2941 tgtacgggaa ctcctcgcgc tggaccgaca ggctcagcac gccggctctc cggataagct
     3001 ccgtcttccg cgccttgcgg ccctgcgtcc ctgtgaagaa cgtgatgttg ccgccaggct
     3061 cgtagccgta ccagatcggt acggtcagcg gcggcctgtt gttgtcgctg gcgacgctga
     3121 gtacgcctac gcgaggctgc gcgaggaact gctctcgctc tttctcggtc atctgtcctg
     3181 gcatgatcct ccctgaatct tagggcggtg ccgagtggct cgaaggtcag tcttctagcc
     3241 actcggttat gaacctctcg ctcgcgtgta tgtccacctg cctgagacgc ccggcgccgg
     3301 aattcacgaa cttgtgcggc actcctgccg gcaccacgac gacctggccg ccgcgcgcct
     3361 cgatcacgtc gtctccggcg gtgaaggtag cctcgccctc ctgcaccacg aagacctcct
     3421 cgtagggatg tttgtgcagc ttcgggcctc ttccctgcgg cgcgtctatc acgatgaacg
     3481 agacgttggt atcaccgtgg tggtgtccct cgaactcgta agcggtctcg ctgtgcgcca
     3541 ggtcggccac gtcgataatg cgcgcctcac tctcctcatg agcccgactg ctacttgctt
     3601 ccggtcctgc gttcacatcg ccctccttca ttggcggtgg tgctcacgac actaaaccca
     3661 accaggctgc tgcagaaacg cacaatcg
//
LOCUS       DATAIR010000363         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_241690, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000363 DATAIR010000000
VERSION     DATAIR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241690"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VHM69_08885"
     CDS             <1..795
                     /locus_tag="VHM69_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2740548.1"
                     /translation="NGGRLLFGGMARWDGLDLFGPNYMSVAYRRKGEIHVKVEPSKMR
                     KPTNPTVQRVSRWPIIRSLFLWGRIFAQVIGSLWTLLFFAATMAVLWLFVRLMQLGSG
                     EGALGGVFAFFAEFPILPILLIFFAAMKFTPIGRYHGAEHKAVAAYEKYGEVTLQNAR
                     SSSRIHPRCGTNILLYILLAALLDPLIAWWGYAILQFILISEAWFIFGQTRPSIAIGN
                     FLQRYITTTEPRRAQLEVAVESLNRLLLAEEEREDGMAPVRVPARY"
     gene            complement(904..1650)
                     /locus_tag="VHM69_08890"
     CDS             complement(904..1650)
                     /locus_tag="VHM69_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740549.1"
                     /translation="MAKLDGKVAVVTGASSGIGEATAEALAAQGAAVVVAARREDRLT
                     DLVERIEGAGGRVLASVCDVTDEEGAHSLIQTAEEEFGSVDILVNNAGVMLLSTVGKG
                     LSDEWRRMFDVNVMGLLYATDAAVEVMKRQGRGHLVNISSVAGRKVTRDSSGVYAGTK
                     HAVGAISEGLRQELLDDNIRVTVVEPGAVETELPDHITDEDARESLGGLLNLEILQAE
                     DIANAVVYAVTQPERVSVNEILIRPTQQPV"
     gene            complement(1689..2642)
                     /locus_tag="VHM69_08895"
     CDS             complement(1689..2642)
                     /locus_tag="VHM69_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740550.1"
                     /translation="MNYGTLIGDAFRITLSNRYLWFFGLFAGGTGSSFVPNVPSTGGG
                     NFNPDDFQQSSTGFSAFTAQVGQGVFGNMVLILGIVVVAILIVLFLIMMAIISHGALA
                     ESVAAIDRGERRRFGSTFRAGMANFWRVLGYYVVFFLVAIGLLVVIGIPVAILIGGTF
                     AATESTGARVSVAVIVGILAVLLLIVVFIPLSIIAQYALREVVVRRARVLGSVGSGYS
                     IFRHNIGRSLLIWLIQIGLSIGIAIAFILAFLIVGLILFIPTIALAIAEYSTAAIIAG
                     VIAGLILLPILLVAAGAIGTFSHAYWTLAYLRLTAPSTTVR"
     gene            complement(2639..>3186)
                     /locus_tag="VHM69_08900"
     CDS             complement(2639..>3186)
                     /locus_tag="VHM69_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX2740551.1"
                     /translation="HIEGKTHVLFAPNSEIIDDKGVGAFVPEMVRRYLGEEPLIENAR
                     TWSLAVEEDRRYVMNHFDELVVKSRGGYGGKEVMIGPEESKEAVEEFRGMVEIDPVEY
                     VAQETIDFSTHVLCETGEDDFLLRDSYADYRVLVLSPDPTDPDVVEAIPGSLSRVAAP
                     GKHVVNISSGGKMKDTWVLAR"
BASE COUNT          599 a         1054 c          924 g          609 t
ORIGIN      
        1 aacggagggc gcctgctctt cggtgggatg gcccgctggg acgggctcga cctcttcggc
       61 ccgaactaca tgagcgtcgc gtaccgccgg aagggtgaga tccacgtcaa ggtcgaaccc
      121 tccaaaatgc gcaagcccac gaaccccacc gtgcaacgcg tctcgcgctg gccgataata
      181 cgctctttgt ttctctgggg cagaatcttc gcccaggtga tcgggtccct ctggaccctc
      241 ctgttcttcg cggccacgat ggcggtcctc tggctgttcg tgcgcctgat gcaacttggg
      301 agcggggagg gagctttggg cggtgtcttc gccttcttcg cggagttccc catcctgccg
      361 atcctgctca tcttcttcgc ggccatgaag tttaccccca tcggccgcta ccacggagcc
      421 gaacacaagg ccgtcgccgc ctacgagaag tacggtgagg tgacgctcca gaacgccaga
      481 agcagcagcc gcatccaccc ccgctgcggt acgaacatcc tgctctacat actcctcgca
      541 gccctcctcg acccgctgat tgcgtggtgg ggctacgcca tccttcagtt catcctcatc
      601 tcagaagcct ggttcatctt cggccagacc cgcccctcga tagccatagg caacttcctg
      661 cagagataca tcaccaccac cgagccccgc cgcgcccagc tcgaggtcgc ggtcgagtcc
      721 ttaaacagac tcctcctggc tgaggaagag cgcgaggacg gcatggcgcc cgtacgggtg
      781 cccgccagat actaaccacg ccatcgcgac tggcacgaac cttgactgct ctcagccggc
      841 gagacctacc tgaatgacgt ggtccctgtc atagggcaac accatgtcag caagtcgtgc
      901 ctgctagacg ggctgctggg tcgggcggat caggatctcg ttcacgctca cccgctcggg
      961 ctgggtaaca gcgtagacga ccgcgtttgc gatgtcctcg gcctgcagga tctccagatt
     1021 caggaggccg ccgaggctct ctctggcgtc ctcgtcggtg atgtggtcgg ggagctccgt
     1081 ctcgacggct ccgggctcga cgacggtcac cctgatgttg tcatcgagta gctcctgcct
     1141 caggccctcg gagatggctc ctacggcgtg tttggtccct gcgtagactc cgctcgagtc
     1201 gcgcgtcacc ttcctacccg ccactgaact gatgttgacg aggtgcccgc ggccctggcg
     1261 cttcatcacc tccacggctg catccgtggc gtagagaagt cccatcacgt tgacgtcgaa
     1321 catcctgcgc cactcgtccg agaggccctt cccgacggtc gatagcagca ttacgcccgc
     1381 gttgttcacg agtatgtcca cgctgccgaa ctcctcctcg gctgtctgga tcaggctgtg
     1441 cgccccttct tcgtccgtga cgtcgcagac ggacgcgagc accctgcccc cggcgccctc
     1501 gatgcgctcc acgaggtcag tgagcctgtc ctccctcctg gccgcgacga ccacggcagc
     1561 tccctgtgcc gccagcgcct ccgccgtcgc ctcgccgata ccgctcgaag cgcccgtaac
     1621 aaccgccacc ttaccgtcaa gcttcgccat gaagtaagcc tttctagacg tttgcccgca
     1681 gcctgatcct atctcacggt cgtggatggg gccgtcaggc gcaggtaggc gagcgtccag
     1741 tatgcgtgtg agaaggtgcc tatcgcgcca gcggcgacga gcaggatggg gagcaggatc
     1801 aggcccgcta ttacgcctgc gatgatggcc gccgtcgagt attccgcgat ggccagcgcg
     1861 atcgtcggga tgaacaggat caggccgacg atcaggaacg ccaggatgaa ggctatggcg
     1921 atcccgatgg agagcccgat ctggatgagc cagatcagca agctcctgcc gatgttgtgt
     1981 ctgaagatac tgtagccgct cccgacggag ccgaggacgc gcgcgcgacg caccacgacc
     2041 tcccgtaacg cgtactgggc gattatggac agggggatga aaacgacgat cagcaacaga
     2101 acggccagta tccccacgat cacagcgacg gagaccctcg ctcccgttga ctcggtggcc
     2161 gcgaacgtgc ccccgatcaa gatcgccacc ggaatcccga tgaccacgag tagcccgatc
     2221 gcgacaagga agaaaaccac gtagtagccg agcacgcgcc agaagttcgc catgcccgcc
     2281 ctgaaggtcg acccgaacct gcgtctctcg cctctgtcta tggcggcgac gctttcggcc
     2341 aaagctccgt gggagattat tgccatcatg atcaagaaca gcacgatcag gatcgccacc
     2401 acgacgatgc caaggatcaa gaccatgttg ccgaacaccc cctgcccgac ctgggccgtg
     2461 aacgcagaga aaccggtgct cgactgttgg aagtcgtcag ggttgaagtt gccccctccc
     2521 gtggaaggga cgttggggac gaagcttgaa ccagtacctc ccgcgaatag accgaagaac
     2581 cagaggtagc ggttgctgag agtgatcctg aacgcgtctc ctatgagtgt tccgtagttc
     2641 atcgcgcgag cacccaggtg tctttcatct tgcctccact ggagatgttg acgacgtgct
     2701 ttccgggagc ggcgacccgg ctcaaggaac cggggatagc ctcgacgacg tcagggtcgg
     2761 taggatcggg ggagagaacg aggactcgat agtccgcgta tgagtcgcgc aggaggaaat
     2821 catcctcgcc ggtctcgcag aggacgtggg tcgagaaatc tatcgtctcc tgggccacgt
     2881 actcgacagg gtcgatctcg accatcccgc ggaactcctc gaccgcctcc ttcgactcct
     2941 caggcccgat cataacctct ttccctccgt atccccccct gctctttacg acgagctcgt
     3001 cgaagtgatt catgacgtag cgcctgtcct cttctaccgc gagcgaccag gtacgggcgt
     3061 tctctatgag gggctcctct ccgaggtagc ggcgcaccat ctcgggtacg aaggctccaa
     3121 ctcccttatc gtctattatc tccgaatttg gagcaaagag cacgtgtgtt tttccctcga
     3181 tgtgag
//
LOCUS       DATAIR010000364         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_56846, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000364 DATAIR010000000
VERSION     DATAIR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56846"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VHM69_08905"
     CDS             <1..1091
                     /locus_tag="VHM69_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740552.1"
                     /translation="VAALLPFLISERGISLAAAGALVFAATVSSSIVQPIFGIFSDRR
                     PIPALMPLGVLLAGSGMALVGVAPSYPLILLCVVASGIGVAAFHPEAARFANYVSGSG
                     RARGMSFFSVGGNAGFALGPALTTPLVLLFGLHGALFLVLPAAVMAAVLTHEIPRMQG
                     FMPDPTEREVRKVADAPEHWRPFALMIGVVTVRSFVYFGLVAFVASYYERVLDMSPAL
                     GNAALTLMLFGGAAGTLIMGPLADRFGRRAVVVASMLLLPPLIFCFTLAGPLAGMAVL
                     VLVGATTVGTFGITVVMGQEYLPGRIGLAAGITMGLSIGLGGIGAPLLGLVADTGGLH
                     LTMLIIAVLPAIGLLLALALPRGTRAHA"
     gene            1190..3763
                     /locus_tag="VHM69_08910"
     CDS             1190..3763
                     /locus_tag="VHM69_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2740553.1"
                     /translation="MRVFHRIGVFGYRFRWAILLLWGALLISSAFFAPELSGRLKGGG
                     FEGSNSEAERVQDLMSGEFGLSPATLTVVFTGDGIPARSKEFQKAQEDALSGVRKLEH
                     VRFVASYAGSKDPRFISEDGEKSYALVAFDVSIDETRNAVAEVRSEVRSDELETYVTG
                     APAVYQDLEKASNEDVKHAEKYAFPFAVVILVFAFGTLVAAGVPVLIGGASVLTALAA
                     LYFITGFYDMSVFTLTLSTMLGLGLGIDYALFFVSRFREEMEDYPTSEAIPRTVATAG
                     RSIFFSGTAVLIGLSGLLFFPFMFMRSIGVAGVVVVFVSVSAALTLLPALLGVLGPRV
                     NRLAIRRRGGGPGTAFWSRSAEVVMRHPVAVILLAAAILGTLLYPVGHMRVGIPEATV
                     LPQKYESRAGDDILKENFEYASLNPMEIVATIDEDPLGAAGLSDTKELGERLRGAEGV
                     SRVESVYTLGEEAAKDYTNRVASAREQANAEAARQVDGLVRRQIEEQTQERTDEAVQQ
                     QLSQIRATYGTVPPGTEEQVRAEVEPRVAKEVRASEGRIRDEVERRVAEEVDRKVPDL
                     PEGISADRVTPEGVANFLELPAARESRELQNTLDSYVAGERTLLRAVTEANPYTQDAY
                     GAVDAVRAVDAPEGTSFLVGGLSAGQKDFISSLYGKAPLAAAFVIGVTYLILLITFRS
                     VFIPLKAVIVNILSLTASFGAMVFVFQDGHLSGLLNFTPLGFVDATLPILMFCTIFGV
                     SMDYEVFLLSRIREAYENGDSNSASVSKGLVATAGIITSAAAIIMVVTGAFAFTGIVL
                     TKAIGLGLAVAVFVDATIIRVLLVPATMRILGDWNWWPGGRRVTFGAGPKEPARSHDS
                     D"
     gene            3852..4262
                     /locus_tag="VHM69_08915"
     CDS             3852..4262
                     /locus_tag="VHM69_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2740554.1"
                     /translation="MVEFERILKDKGYRLTNQRRIIVRELEGERHLSAEEIYDRVKGE
                     HPELGLSTVYRTLDLLTELGIVRKDDFGEGYSRYELTTERVHHHARCRECGSVIEFNE
                     ELMEYLALQVERETGFVTEWHEITLHGRCANCSA"
     gene            4378..>5006
                     /locus_tag="VHM69_08920"
     CDS             4378..>5006
                     /locus_tag="VHM69_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740555.1"
                     /translation="MKRFSTLVAALVLAVVAVGIFAGSLIAQTSPALYAACRPDQREI
                     EAAELPGVVDQDRCPVGGREIVDGGISVVVPEPGESVFAEAMSPRGGEHLVVSNLAAK
                     TLVLGEVGSESGTQESFMTQSSGPGACRDAHRHSWDSRLYNYLRYYFNRRSTPSHLAD
                     VGVARAIERAGRNVSRVRDSCGIPDRVPAVLKYRGVTRTSVDMDAGGTC"
BASE COUNT          867 a         1523 c         1640 g          976 t
ORIGIN      
        1 cggttgcggc tctgctacct ttcctcatct ccgagagggg catatccctg gccgccgcgg
       61 gcgccctggt gttcgccgcg acggtgagct cgtcgatagt gcagccaatc ttcggcatct
      121 tctccgacag gcggcccata cccgccctga tgccgctcgg cgtgttgctc gccggaagtg
      181 gcatggccct cgtcggtgtg gccccgagct atccactgat cctgctatgt gtcgtggcga
      241 gtgggatcgg ggtggccgca ttccaccccg aggccgcgag attcgccaac tatgtgtccg
      301 gctctggccg ggcgcggggg atgagcttct tctccgtcgg agggaacgct ggcttcgccc
      361 tgggtcccgc gctgaccaca ccgctcgtac ttctcttcgg cctgcacgga gccctgttcc
      421 tggtcctgcc agcggccgtc atggccgccg tcctgacgca tgagatccca cggatgcaag
      481 ggtttatgcc agacccaacc gagagagaag tacggaaagt ggccgacgcg ccggagcact
      541 ggagaccttt cgcactcatg atcggtgtgg tcacggtcag gtctttcgtc tacttcgggc
      601 tcgttgcctt cgtcgcctct tactacgagc gtgtcctcga catgtcacct gccctcggga
      661 acgcggccct cacgctgatg ctcttcggcg gtgcggcggg gactttgatc atgggtcccc
      721 tcgcagacag gttcggaagg cgcgcggtcg tcgtagcctc gatgctgctg ttgccgcccc
      781 tgatcttctg cttcacgctg gccggtccgc ttgcaggcat ggctgtactc gtcctcgtcg
      841 gagcgactac cgtggggacg ttcgggatca cagtagttat gggacaggag tacctgcccg
      901 gacgtatcgg actcgcagcg ggtatcacga tgggtctctc catcgggctc ggcggcatcg
      961 gggccccgct actcggcctg gtcgcggata cgggcgggct ccatctgact atgctgatca
     1021 tcgcggttct cccggccatc ggcctcctcc tcgccctggc actgccccgc gggacgcggg
     1081 ctcacgcctg agaggaaccc ggttcgtgta accaaacacc atcgtgtccc cgcgggtcga
     1141 gtaccatatc gccgatgaga gcgggtttta gagagggtca cctgcaaggg tgagggtatt
     1201 ccatcgcatc ggcgtcttcg gctaccgttt ccggtgggct attctgctgc tctggggcgc
     1261 gctccttatc tccagcgcgt tcttcgcacc cgaactctcg ggacggctga agggcggcgg
     1321 gttcgaggga tccaactcag aggcagagag ggtgcaggac ctgatgtccg gcgagttcgg
     1381 gctctcccca gcgaccctga cggttgtgtt cacgggcgat ggcatccctg cccgcagcaa
     1441 ggagttccag aaggcccagg aggatgcgct ctcgggagtt cgcaagctgg aacacgtacg
     1501 cttcgtcgcg tcctacgccg gatccaaaga tcccagattc atctccgagg acggcgagaa
     1561 gtcctacgcg ttggtcgcct tcgacgtctc catcgacgag acccgcaacg ctgtggcgga
     1621 ggtgaggagc gaggtcaggt cggacgagct cgagacttac gtaacaggag cgcctgccgt
     1681 ctaccaggac ctcgaaaaag cgagcaacga ggacgtgaag cacgcggaga agtacgcttt
     1741 cccgttcgcc gtggtcatac tcgtcttcgc cttcggcacg ctcgttgccg ccggtgtgcc
     1801 cgtactgatc gggggcgcga gcgtgctgac cgccctcgcc gcgctgtact tcatcacggg
     1861 tttctacgac atgtccgtgt tcacgttgac cctatcgacg atgctcggtc tcgggctcgg
     1921 aatagattac gcgctgttct tcgtgagccg cttcagggag gagatggagg actacccgac
     1981 gtcggaggcg attccacgta ccgtcgcgac cgcgggacgc tccatcttct tctcagggac
     2041 ggccgtcctg atcgggctct ccggcctgct cttcttccct ttcatgttca tgcgctccat
     2101 cggggtggcc ggcgtcgtcg ttgtcttcgt ctccgtctcg gcggccctga cacttctgcc
     2161 agccctcctc ggcgtcctcg gaccgcgggt gaacaggctc gccataaggc gccgaggtgg
     2221 tggtccagga acggcgttct ggagccgaag cgccgaggtc gtgatgcgcc atcctgtagc
     2281 cgtgatcctg ctcgcggcgg cgatcctcgg caccctgctc tacccggtgg gccacatgag
     2341 ggtcggcatc ccagaagcga ccgtcttgcc gcagaagtac gagtcgcgcg ccggtgacga
     2401 catcctgaag gagaacttcg agtacgcgtc cctgaaccct atggagatcg tcgccacgat
     2461 cgacgaggac ccactgggtg ccgctggtct atccgacaca aaggaactgg gagagaggct
     2521 ccgcggcgcc gagggcgtat cccgcgtcga gagcgtctac acacttgggg aggaggcggc
     2581 gaaggattac acaaaccgcg tcgcaagtgc gcgcgagcag gcgaatgcag aagccgccag
     2641 gcaggtagac gggctcgtgc ggcgtcagat tgaggaacag actcaggaga ggacggacga
     2701 ggcggtgcag cagcaactct cgcagatacg ggcgacctac ggcaccgtac cgccggggac
     2761 cgaggagcag gtgcgggccg aggtggagcc cagggtggcg aaggaggtgc gcgcttcaga
     2821 gggtcgcata agggatgagg tcgagcggcg cgtcgccgag gaagtcgacc gtaaggtgcc
     2881 agatctgcca gaggggatct cggctgacag ggtcacgccc gagggtgtgg cgaacttcct
     2941 cgagctgccc gcggccagag agtcaagaga gttgcagaat acgctggact cctacgtggc
     3001 gggggaaagg acgctgctca gggccgtgac cgaggccaat ccatacacgc aggacgccta
     3061 cggcgcggtc gacgccgtgc gcgcggtcga tgctccagag gggacgagct tcctggttgg
     3121 cgggctctcc gcgggccaga aggacttcat ctccagcctc tacggcaagg cgccactcgc
     3181 agctgccttc gtcatcgggg ttacgtacct gatccttctc ataaccttcc gctccgtgtt
     3241 catcccgctg aaggcggtca tcgtgaatat cctgagcctg accgcaagct tcggggctat
     3301 ggtcttcgtg tttcaagatg gtcatctctc cggcctcctc aacttcaccc cccttggatt
     3361 cgttgacgcg acgctcccta tcctgatgtt ctgtacgatc ttcggggtct cgatggacta
     3421 cgaggtcttc ctcctctccc gcatccgtga agcctacgag aacggcgact cgaactccgc
     3481 gagcgtctcg aaaggcctgg tcgcgaccgc ggggatcatc acctcagcag ccgcgatcat
     3541 tatggtagtg acaggagcct tcgcattcac ggggatcgtc cttaccaaag ccatcgggct
     3601 cggcctggcc gtcgccgtat tcgtggacgc gacgatcatc cgcgttctcc tcgtacccgc
     3661 aaccatgcgc atcctcggcg actggaactg gtggcccggt ggacgcaggg ttactttcgg
     3721 ggccggcccg aaggagcctg cgagatcaca tgacagcgac tgatgagttt tccggatgtc
     3781 gtcagaatgt tttcgcctca agataagatc aattcttgat cttgctgtgt accggggata
     3841 gactgtagcc catggtggag ttcgagcgga tactcaagga caagggctac cggctcacca
     3901 accagcggcg gatcatcgta cgcgagctgg agggggagag gcatctctcg gccgaggaga
     3961 tctacgaccg ggtcaaaggg gagcatcccg agctcggcct ctccacggtg tacaggacgc
     4021 tggatcttct gacggagctg ggcatcgttc ggaaggacga tttcggcgag ggttactcgc
     4081 gctacgagct aacgaccgag cgtgtgcacc accacgcgcg ttgcagggag tgcggttcgg
     4141 tcatcgagtt caacgaggag ctcatggagt acctggcctt gcaggtcgag cgggagaccg
     4201 gtttcgtcac cgagtggcac gagatcacac tgcacggccg ctgcgcgaac tgctcggcgt
     4261 aaaggcgagc gcggggcgac tttttcgttt tcaccgttga ttttggtcag catatctggc
     4321 taaagtgtat gggctgcaca acatgtagta tgtgaccagc gccctggagg gggaaatgtg
     4381 aagcggttca gcactttggt cgcggccctg gtattggcgg ttgtggccgt aggaatattc
     4441 gccgggtctc tcatcgcgca gacgagcccg gctttatacg cggcctgcag gcccgaccag
     4501 cgtgagattg aagcagccga gcttcccggc gtcgtcgacc aggacaggtg ccctgtcggt
     4561 ggaagggaga tagttgatgg cgggatctcc gtggtcgttc ccgagcctgg ggagagcgtc
     4621 ttcgccgagg cgatgtcgcc gcggggcggc gaacacctgg tggtcagcaa ccttgctgcc
     4681 aagacgctcg tcctgggtga agtcgggagc gaatcaggga cccaagaatc gtttatgacc
     4741 cagtcctccg gaccaggagc gtgcagagac gcgcaccgcc attcgtggga ctcgcgactg
     4801 tacaactatc tccgctacta cttcaaccgg aggagcacgc cctcacacct ggccgacgtg
     4861 ggggtagcgc gcgcgataga gagggctggg cgcaacgtca gcagggtgag ggacagttgc
     4921 ggcataccgg acagagtgcc ggccgtcttg aagtaccggg gagttacacg aacatccgtt
     4981 gacatggacg cgggcggcac ctgtaa
//
LOCUS       DATAIR010000365         9156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_300779, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000365 DATAIR010000000
VERSION     DATAIR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300779"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VHM69_08925"
     CDS             complement(<1..670)
                     /locus_tag="VHM69_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2740556.1"
                     /translation="MRESAHRITIADVAREAGVSRQTVSRVLNGKGEIRASTRESVMR
                     VIERLGYSPSGIARSLATNKTLTVGLVVPDITNPFFPEIARGVEDVARERGYEMFLCN
                     SGEDPEREDDVLRALQDKRVDGTIICSSRLSDERLFTRLRQQRAAVLVNRHAPPELAG
                     TVRVDDAEGTFQAVDHLLNGGRCVIGFLCGPPNSRSGIERVRGFESALSAAGTGLDES
                     LMRPC"
     gene            749..2095
                     /locus_tag="VHM69_08930"
     CDS             749..2095
                     /locus_tag="VHM69_08930"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740557.1"
                     /translation="MEGHGGPRIAFVGGGSYQWGPKIILDVALNEELRGGTLVLHDIN
                     NEALDDMYAWGTRAAAEANADLTLEKSLRLDEALEGADFVVLSISTGGLDATALDLEI
                     PTKYGVVQTVGDTVGPGGHFRALRNIPVVVDIAKAMERVCPDAVLLNLTNPLTALTRA
                     VTKATSIRAVGLCHELFSTLGMLSKMFDAPEDVINVRVAGVNHFIWVTDVSVRGRDVT
                     EEAFSRISGGEARETALDDAAGDTDPFLNTWGFRTELCRLYGYLPAAGDRHLCEFLPG
                     YLQDEKERERLDLRVTTVDIRREKLTADRERVRRMIQGQEPIPTGRSREEISDIMAAM
                     WTGKDSVNIVNLPNEGQVRDLPLGAVVETYGALNGAGASGITFGELPSPVAALVHPHV
                     FNQEATVHAGLTGDRDLAYRAFLNDPLVGSRPDAGRMFEEMFDAQAAYLPQFGASP"
     gene            complement(2522..2776)
                     /locus_tag="VHM69_08935"
     CDS             complement(2522..2776)
                     /locus_tag="VHM69_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740558.1"
                     /translation="MTVEELLPVASGLVLGSILGLLRSSLRLPVGLVLIVVLGVAATV
                     VSGEYLISWSFLLIDIPLVAVSAVAGLYVTHRLVWAGRRR"
     gene            complement(2773..3099)
                     /locus_tag="VHM69_08940"
     CDS             complement(2773..3099)
                     /locus_tag="VHM69_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740559.1"
                     /translation="MVYIVIKTDGAEAAVTTQEGEETPTQATAAPGAAPPSEVLAAAL
                     AAGAQDAGPGPADAAPTTTGAPPLPLQAATPGAPGAGSDVLSAGAAPGARPEPPPTTV
                     TAEDGE"
     gene            complement(3117..3371)
                     /locus_tag="VHM69_08945"
     CDS             complement(3117..3371)
                     /locus_tag="VHM69_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740560.1"
                     /translation="MWTGTQFTGTLGPGASNQWFTHSWPTSWHVVWYMMPTSPQSGAP
                     QVDWDVRVERANSTQCTYWLTVKNLTANTVTFEGRFAVLN"
     gene            complement(3402..5969)
                     /locus_tag="VHM69_08950"
     CDS             complement(3402..5969)
                     /locus_tag="VHM69_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740561.1"
                     /translation="MRGRLDDAIETKRARHEDRRQRRRAPGGKPLVRLLQILERSGYP
                     RTAGNSLRIALPEEATEEFTEEAARFTATPSGEVPGPAPAAEGETEEEQSATVAEAVS
                     QLYIETSNQLGVPTPTGPQWRSLGPWTIPNGQTYGSSRVNVSGRISAIAVDPLNPAHV
                     LAGAAGGGVWESKDRGASWAPRTDYAATLTVGAIAFDPKSPSTVYCATGEGNFYWYLG
                     AGILRSTDGGTTWSTLCTAPFVGQGFYDLIVDPANSQHLLAGTTGGLYVSTNGGVNWT
                     RRRTATTWSLSMAPAGGASAEILAGCSDGVRRSTDGGTTWTAVTLPGAPATFDRLAVA
                     IARSSPSVAYAWGSSGGTAYLRRRASGTWTTVAAPPGVSTGQAWYDWFLAVAPDVSTQ
                     IYCGAIEVHRGDLSGTTWTWRNLTNKGTIGSSIHPDQHAMAFEPGQPNTIYVGNDGGL
                     YRSPDRGITWAHCNNGLVISEFEFLAHDYGSSRWLIGGTQDNGTQRWTGSSVWDHVAD
                     GDGGDCGVNRTNPRTVYHTYYGMSPERSTTGGNFGSWTYIPPPVPAGEDSLFYPPFEC
                     SASGGDTIAIGGDALYISRNNGTAWVRLTFPSAARSSAMYIRNTNTVHVGTTDGRIFR
                     TTWNGVSWTALTALTTPRSNAAMSDIYVDAANANRMWVTYSTTGGGRVYRSDNGGSSW
                     IDRTAGLPGLPINAVEVDPGNANRVWVAADLGVYQSTDGGTTWADFSNALPNALVGDL
                     LFHPHARVLRAGTRNRGVWEIPVDGWMTQPQCGVQWTGSLVGNEQRRWFTHSWPATWH
                     VLWTVMPTTPRPGAPQVSWSVQVERATAEYATYWITVTNLTPDPMSFEGRYCILSRY"
     gene            6450..6650
                     /locus_tag="VHM69_08955"
     CDS             6450..6650
                     /locus_tag="VHM69_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740562.1"
                     /translation="MPRRPGDTEPEPPGGRAAKRLREFINQRFPGGSPPREGKEERDR
                     NEKERRKREESDAEDRESGNTR"
     gene            complement(7498..8331)
                     /locus_tag="VHM69_08960"
     CDS             complement(7498..8331)
                     /locus_tag="VHM69_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740563.1"
                     /translation="MTVPAHATIAEDALRSALSSGERPKRPNALSTSLTFGWRALLKI
                     KHVPEQLFDAVGNPIIFTLLFTYLLGGAIAGSTGAYLQFLIPGILVQTVLLITAYTAI
                     NLNTDFDKGVADRFRSLPIWRPSVIVGALLGDMARYTLASAIVLALGLVLGYRAEGGV
                     VGMLLSVVLLVVFAFSVSWIWTALGLVLRTPTSVQWVSVLILFILAFASNVFVDPKTM
                     PGWLETAVGYNPITHLVIAVRGLMSGTVPVGHIAWLLVGCAVLVAVFAPITMRLYRNK
                     E"
     gene            complement(8328..>9156)
                     /locus_tag="VHM69_08965"
     CDS             complement(8328..>9156)
                     /locus_tag="VHM69_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2740564.1"
                     /translation="RMLATLLRPHSGTARVFGHDVVRESDAVRGRVSLTGQFASVDED
                     LTGYENLVLVARLLGYSRKGAKGRAGELLDAFGLSEAAGRLVKTYSGGMRRRLDIAAS
                     IVVTPDLLFLDEPTTGLDPRNRNQVWDIVRAMSSGGTTVLLTTQYLDEADQLADRIAV
                     IDNGKVIAEGTSGELKNSVGTGVLRVRLREPEARPEAQRVLSRALDVAVETTSDPVAL
                     LARVSEPERAPGALVELPRAGITVTDFALGQPSLDEVFLALTGHTTKQQADKEEETA"
BASE COUNT         1663 a         2902 c         2950 g         1641 t
ORIGIN      
        1 gacacggacg catgagtgac tcgtcgagac cagtaccggc cgccgacagt gccgactcga
       61 agccccgcac acgttctatg ccgctgcgcg agttcggtgg accgcacaga aacccgataa
      121 cgcagcgccc gccattcagc aggtgatcca ccgcctgaaa cgtaccttcg gcgtcatcca
      181 cccgaaccgt tcctgccagt tcgggtggcg catgcctgtt gacaaggacc gccgcccgtt
      241 gctgccgcag gcgggtgaag agccgctcat cggacagcct cgaactgcag atgatggtcc
      301 cgtccaccct cttgtcctgg agggcccgca ggacatcgtc ttcgcgctcg gggtcctcgc
      361 cgctgttgca caggaacatc tcgtaaccgc gctccctcgc cacgtcctcg accccgcgcg
      421 cgatctccgg gaagaaagga ttcgttatgt cgggcacgac cagccctacg gtgagggtct
      481 tgttcgtggc caggctgcgc gcgatgccgc tcgggctgta cccgaggcgt tcgatcacac
      541 gcataaccga ctcgcgggtc gaggcacgga tctccccctt cccgttgagt accctcgaaa
      601 ccgtctgcct tgaaaccccc gcctcacggg ccacgtcggc gatggtgatc ctgtgcgcgc
      661 tctccctcac tctacccctt tgttaccgct cacgtgtacg gtatcatagc tctctgaacc
      721 tgtcagagac gggcataggg agggcaatgt ggaagggcac ggcggaccca ggatagcctt
      781 cgtaggcggc ggaagttatc agtgggggcc caagatcatc ctcgacgtcg cgttgaacga
      841 ggagttgcgc gggggcactc tggtcctcca cgacatcaat aacgaggcgc tcgacgacat
      901 gtatgcctgg gggaccaggg ccgcagcaga ggccaacgcc gacctgacgt tggagaaatc
      961 gctacgattg gatgaggccc tcgaaggggc tgacttcgtt gtgttgagca tctcgaccgg
     1021 tggccttgac gctacggccc tggacctgga gatccccacc aaatacggcg tagtgcaaac
     1081 cgtgggcgac accgtggggc ctggcggcca cttccgcgcc ttgcggaaca tacctgttgt
     1141 ggtggatata gcgaaggcga tggagagggt ctgtccagat gcggtactgc tcaacctgac
     1201 caacccactc acagccctca cgagggccgt cacgaaggcg acctctatca gggccgtcgg
     1261 gctctgtcac gagctattca gcaccctcgg catgctctcg aagatgttcg atgcgcccga
     1321 ggacgttatc aacgtcaggg ttgccggagt aaatcacttc atctgggtca cggacgtttc
     1381 agtacgaggc cgcgacgtca ccgaagaggc gttcagccgc atctcaggtg gcgaggcgag
     1441 agaaacggct ctggacgatg ccgcgggcga cacggacccc ttcttgaaca catggggctt
     1501 tcgcaccgag ctctgcagac tgtacggata tctgccggcc gcgggcgacc ggcacctatg
     1561 cgagttcctt ccagggtacc ttcaagacga aaaggagcga gagaggctcg accttcgggt
     1621 aacaacggtt gacatcagga gggagaagct caccgcggac agggagaggg tgcggcgtat
     1681 gatccagggc caggagccca tcccgacggg ccgctcgcga gaggagatct ccgacatcat
     1741 ggcagccatg tggaccggca aggactcggt caacatcgtc aacctgccga acgagggcca
     1801 ggtcagggac cttccccttg gtgccgtcgt cgagacgtac ggcgcactaa acggcgcggg
     1861 ggcttcaggc atcacgttcg gggagcttcc ctcgccagta gccgccctcg tgcacccgca
     1921 cgtcttcaac caggaagcga ccgtccatgc tggtttgacg ggggatagag atctcgcgta
     1981 cagggcattc ctcaatgatc ccctcgtcgg gagccggcca gatgcgggcc ggatgttcga
     2041 agagatgttc gatgctcagg ctgcgtacct gccccagttc ggagcctctc cgtagacgtg
     2101 ccaacaagcg tcaggggtgt tcctggaact gtgcagttgc gaccttgcga gtcgcggaag
     2161 ctgtggggtt gtaacagcgg cttgcggaag gattgatcct ccccaagctg ggcttcaggt
     2221 ttcaggcatc aggaaggtct tcccggactg acggtggtcg ctaaggtgcg cctctggggc
     2281 gaggtgggtc aatacttatg tactacgctg taagcacaaa ggcctgcgcg tgagttcgag
     2341 tcttggtcat gtggcgacgc acgcgaaccg gaggcgcctc cagccgggac gcagacgaag
     2401 tgaaccgcgc tgtggaccca tgcgcgtgcc acgacgccag aaccagaatt ctatgacgta
     2461 gtattaccgg actctctcaa ggagcaaagc agggcgcgac tatgcggctg gtccttctac
     2521 attacctccg tctgccggcc cacaccaggc gatgcgtgac gtacagaccg gccacggctg
     2581 agacggcgac gagtgggatg tcgatcaaga ggaacgacca gctgatcagg tactccccgc
     2641 tcaccacggt ggctgccacg ccaaggacga cgatgagcac aaggccgacg ggcagccgca
     2701 gggaggagcg gagcaggccg aggatagagc cgaggacgag gccgctggcg acgggcagta
     2761 actcctccac ggtcactcac cgtcctctgc ggtgacggta gtaggcggtg gctctggcct
     2821 ggcgcctggc gccgctccgg cgctgaggac gtccgatccg gccccgggag cacccggcgt
     2881 tgccgcctgg agaggcagtg gaggcgcgcc tgttgtggtc ggcgccgcgt cggcgggtcc
     2941 agggcccgcg tcctgcgcgc cagcagcgag agccgccgca agcacctcac tcggcggggc
     3001 cgcgccggga gccgccgttg cctgcgttgg ggtctcctcg ccctcctgcg tcgtcacggc
     3061 ggcctccgcg ccgtcggtct tgattacgat gtataccatg tcgcaacctt cctctttcag
     3121 ttcaggacgg cgaatcttcc ttcgaaggta accgtattcg ccgtcaagtt cttgacggtg
     3181 agccagtacg tgcactgcgt agagttcgcc cgctcgacgc ggacgtccca atcgacctgc
     3241 ggggcgcccg actgtgggga ggtcggcatc atgtaccaga cgacgtgcca cgacgtcggc
     3301 cagctatgcg tgaaccactg gttcgatgca ccgggaccga gtgttcccgt aaactgtgtg
     3361 cccgtccaca tatctcgtct ccttccttgg ttattggcta ctcaatagcg gctcagaatg
     3421 cagtatcgtc cctcgaagct catagggtcg ggggtgaggt tggtcaccgt gatccagtac
     3481 gtcgcgtact cggcggtcgc ccgctcgacc tggacggacc acgacacctg cggggcgcct
     3541 ggtcgcgggg tcgtcggcat gacggtccag aggacgtgcc aggtcgccgg ccagctgtgt
     3601 gtgaaccagc ggcgctgctc gttgcccacg aggctgcccg tccactgcac gccgcactgc
     3661 ggctgcgtca tccacccgtc gaccgggatc tcccagacgc cgcgattgcg tgtgccggcc
     3721 cgcagcacgc gcgcgtgcgg atggaagagg aggtcgccga ccagggcgtt cggaagggcg
     3781 ttcgaaaaat ccgcccaggt cgtaccgccg tcggtgctct ggtacacgcc gaggtcggcg
     3841 gcgacccaga ccctgttcgc attcccggga tccacctcga ccgcgtttat gggcaaccct
     3901 ggcaggcccg cggtgcgatc gatccacgat gagccgccat tatctgagcg gtagacgcgc
     3961 ccgccgccgg tggtgctgta agtcacccac atgcggttgg cgttggccgc atcgacgtag
     4021 atatcgctca tcgccgcgtt tgaacgaggc gtcgtcagcg ctgtgagcgc cgtccagctc
     4081 acgccgttcc aggtcgtcct gaagatgcgg ccatccgtgg ttccaacgtg gacggtattg
     4141 gtgttgcgga tgtacatggc gctcgagcgg gccgcgctcg ggaacgtgag gcgcacccac
     4201 gccgtgccgt tgttgcgcga gatgtagagg gcatctcccc cgatcgcgat ggtgtcgccg
     4261 cccgaagcgc tgcactcgaa cgggggatag aagaggctgt cctctcccgc ggggacaggc
     4321 ggcgggatat aggtccatga accgaagttc ccgccggtgg tcgaccgctc agggctcatg
     4381 ccgtagtacg tgtggtacac ggttctcggg ttcgtgcggt tgacgccgca gtcgccaccg
     4441 tcgccgtctg ccacgtggtc ccacaccgag gagcccgtcc agcgctgggt gccgttgtcc
     4501 tgcgtaccac cgatcaacca cctggatgag ccgtaatcgt gcgcgaggaa ctcgaactcc
     4561 gagatgacga ggccgttatt gcagtgcgcc caggtaatgc cacggtccgg gctcctgtag
     4621 agcccgccgt cgttaccgac atagatagta ttcggctgcc ccggctcgaa agccatggcg
     4681 tgctggtcgg ggtggataga ggaccctatc gtgcccttgt tggtgaggtt gcgccaggtc
     4741 cacgtcgttc cggagaggtc tccgcggtgg acctcgatgg cgccgcagta gatctgggtg
     4801 ctcacgtcag gcgccaccgc gaggaaccag tcataccagg cctggccggt gctcacgccc
     4861 ggcggcgcgg ccaccgtggt ccacgtcccc gatgcgcgcc gcctgaggta cgccgtgccg
     4921 ccgctcgatc cccatgcgta ggcgacagac gggctcgaac gcgcgatcgc aaccgcgagc
     4981 ctgtcgaagg tcgcaggcgc gccaggcaga gttacggcgg tccaagtcgt cccgccgtcg
     5041 gtagagcgcc gcacgccgtc actgcacccg gcgaggatct ccgccgacgc gccgcccgcg
     5101 ggagccatcg acagcgacca cgtggtcgcc gtgcgccgcc tggtccagtt cacgcccccg
     5161 ttggtggaga cgtagagccc gcccgttgtg cccgcgagca ggtgctggct gttggccggg
     5221 tcgacgatga gatcgtagaa cccctggccg acgaatggcg ccgtgcatag cgtcgaccac
     5281 gtggtcccgc cgtccgtgga acgcaagatc ccggcgccca ggtaccagta gaagttccct
     5341 tcaccagtgg cgcaatacac ggtcgagggg cttttcgggt cgaaagctat ggcaccgacg
     5401 gtgagcgttg cagcatagtc ggtgcggggc gcccagctcg cgccacgatc cttgctctcc
     5461 cacacacctc cgccggcagc cccggcgaga acgtgcgccg gattcagcgg atctaccgct
     5521 atggccgaga tcctacccga gacgtttaca cggctggatc cgtacgtctg cccgttcggg
     5581 atggtccacg gacccaggga ccgccactgg gggccggtgg gcgtcgggac cccgagttga
     5641 ttgctcgtct ctatgtacag ttgggaaact gcctcggcga ccgtggcgct ttgttcctcc
     5701 tcggtttcgc cctctgcggc cggtgcgggg cccggaacct cgccactcgg tgtcgccgtg
     5761 aagcgcgcgg cttcctccgt gaactcctcc gtggcctcct cgggcaaggc gatgcggagg
     5821 gagttgcctg ccgtccgggg ataacccgaa cgttcgagta tctgcagcaa cctgactagc
     5881 ggcttgccgc ccggcgcccg gcgacgttgg cgccgatctt cgtgtcgcgc ccgcttggtc
     5941 tctatggcgt cgtcaagtcg cccacgcata agccctcctc tgtgccaaac tcaagcttgt
     6001 acatgccggt gccgggcagc ggtgaccgca cgcgaataac tacctggcaa gatgacgcac
     6061 ctggcgtcgg atcgcgcttc cagggcgatc atcccgttcc ctcatccttc ccaagacagc
     6121 cacaatccgt catagtagga ggcaacgatg aattcgcgat gaatcaacga tgaattcgcg
     6181 atgaatcaac gatgacatgt gaccctgaac gcgcgatggg caccacattc ggaggttcgt
     6241 gataatttcc gaccggcgta cgccgggttc ttcagtacgg ctcaccaacg tgcacgcctt
     6301 gaccggctga cgctgcgctg ttcctcgaca ctcgatttgt ctttgaagta aaccgtggac
     6361 cgcggactga cccgaaggcg cgtctaacgg tcatcgaccc ggtgctatac tcggagagaa
     6421 gatcggccgt tgtcgagagg agaattagca tgccgcgtcg gcctggcgac accgaaccgg
     6481 aaccaccggg aggtcgcgcc gcaaaacggc taagagagtt catcaaccaa cgcttccctg
     6541 gcgggtcccc acccagagag ggtaaggaag aaagggaccg caacgagaag gagcgcagga
     6601 aacgcgagga gagcgacgcc gaagaccgtg agagcggcaa caccaggtga ctggcaaagt
     6661 atagagtgcc tggtgcaacc gattgcacgt ccgctctgaa cgtgcatcat cacggataga
     6721 tctccactcc acccgtatgt cccggtcgct gcgcccgtgc gatgatggct gctacgggga
     6781 tgttgcccat gcgcgcgaga tgacgcgcgg ggctggaaac gtccacctat cgcatctggt
     6841 gctatagtga tgcaatgttc tcgctagggc ctttagaaac tctcgtgttg gggcctcgga
     6901 ctcccgatcc ttatagtctg cttgctcgtc cgctcacgta gatagtgctg cgttatattg
     6961 atgatgcacg ctgcgcctca cggcttatcc ttgctgttcg gagcgtagag tcgactgctc
     7021 gtagatctcg ttagcaattg gatgactgaa atagaagatg gtttccttgt tgcttccact
     7081 ttggcgacaa cccatgcgtc gcgagcatag cgggagccta ttcttaatgg gtatagaggt
     7141 acagtcctcc gcccgaaaac accctctcaa gcaatgcctt tactcctgtg gtggggcaga
     7201 tcggagaacg ccctccagta gcgcaacaga attgtgtaga gtcccaacca tgacctcacg
     7261 ctcctcagcg catatggcca cgacagcaga ttcgtagtcc cttttcgaag gatgccaccg
     7321 ggcacgcgaa gaggccccgc ccgttcccgg tagtccacct cagtgcgtcg agcggggccc
     7381 tatgagtccg attgacgtgt tcgctactta cacttgatac cgctcgcaac tcgtgccgaa
     7441 tagactctca cccgggtgca taattcctgt cgaggcaacg gcaaagatgt gatccgctca
     7501 ctccttgttc cggtaaaggc gcatagtaat tggggcaaag acggcgacga gcaccgcgca
     7561 ccccacgagc agccaggcga tgtgccccac cggcacggta ccggacatca ggccgcggac
     7621 cgcgatcacc aggtgtgtga tcggattgta gccgacggcc gtttcgagcc agcctggcat
     7681 cgtcttcgga tcgacgaaga cgttgctggc gaaagcgagg atgaacagga tcaggacgct
     7741 gacccactgc acggaggtcg gtgttctgag cacgagcccc agggccgtcc agatccacga
     7801 gacgctgaac gcgaacacca cgagcaacac taccgacagc agcatcccga cgacgcctcc
     7861 ctccgctctg tagccaagca ccagccccag tgcgaggact atcgccgacg cgagggtgta
     7921 acgcgccatg tcgccgagca acgccccaac gatgacggaa ggccgccata tcgggagcga
     7981 ccggaagcgg tcggctacgc ctttgtcgaa gtcggtgttg aggttgatcg ccgtgtaggc
     8041 ggtgatgagc agcacggtct gcaccaggat gccggggatg aggaactgga ggtaagctcc
     8101 ggtcgagccg gcgatggcgc cgcccagcag gtacgtgaac aggagggtga agatgatcgg
     8161 gttcccgacg gcgtcgaaga gctgctcggg gacgtgcttt atcttgagaa gcgcccgcca
     8221 cccgaacgtg agcgaggtag agagggcgtt gggacgcttc ggcctctcgc ccgacgagag
     8281 cgcggaccgc agcgcatctt cggcgatggt ggcatgggct gggacggtca tgcggtttcc
     8341 tcctctttgt cggcctgctg tttggtggta tgtcccgtca gggcgaggaa cacctcgtcc
     8401 aggctcggct gcccgagcgc gaagtccgtc acggtgatgc cggcgcgggg aagctccacg
     8461 agcgcgcctg gtgcccgctc gggctcggag accctggcca ggagagcgac cgggtccgaa
     8521 gtcgtctcca ccgcgacgtc gagcgcccgc gaaagtactc gctgggcctc cgggcgggct
     8581 tccggttcgc gcagcctgac ccgcaggaca cccgtgccaa ccgagttttt gagttcgccg
     8641 gaggtgcctt cggcgatcac cttcccgttg tctatgacgg cgatgcggtc cgcgagttgg
     8701 tccgcttcgt cgaggtactg cgtggtcaaa agtaccgtcg ttcccccgga ggacatggcg
     8761 cggacgatgt cccagacctg gttgcggttc ctggggtcga ggccggtggt cggctcgtcg
     8821 aggaagagga ggtccggcgt gaccactatc gacgccgcga tgtcgagcct gcggcgcatg
     8881 ccacccgagt atgtcttcac cagccggccc gccgcctccg agagcccgaa agcatcgagg
     8941 agttcaccgg cgcgcccttt cgctcccttg cgggagtagc cgaggaggcg ggccacgagg
     9001 acgaggttct cgtagccggt aaggtcctcg tccaccgaag cgaactgccc ggtgaggctc
     9061 acccgacccc gcactgcgtc ggactcccga acgacgtcgt ggccgaacac ccgcgccgtg
     9121 ccggaatgcg gacgtagcag ggtcgcgagc atccga
//
LOCUS       DATAIR010000366         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_23678, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000366 DATAIR010000000
VERSION     DATAIR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23678"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2966)
                     /locus_tag="VHM69_08970"
     CDS             complement(<1..2966)
                     /locus_tag="VHM69_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740565.1"
                     /translation="MNETCGCCEGVEAVTPLSTANRPGLEALSYRVGTHATFLETMIA
                     RLSSAEYPALGGLTTRAANDPSIALLDAWATVADVLTFYQERIANEGYLRTATERRSI
                     LELARLVGYVPRPGVAASVYLALTLEDEYRTEIPAGTRAQSLPGPGELPQFFETSEAL
                     EARSEWNVLTPRLSRPQSLRPITPPGDDTPRGRLYLKGVSTNLGPNDPLLVESGLARR
                     LIKVRTVEPDPERDRTLVTFTLPRPSTVISTRPGIEDIRQRVEGLLDLEAHRVSPESE
                     TTRRVVGLLEDLQTRLTPETPPEAPADSLRETVLPGVRQDLSTARERNYTRLTPWLER
                     IEGELQRIESELASSRLGVARASGGAVSAFSNRAEVNGRLGEGNAPSATLDGLLDRLG
                     KPPSVQPVSAAKLSRSLAKSFGTDSDLVPQLLSALRPDLGRALYQAWEQTGTEPSPVS
                     IYALRVTAAPFGHNAPPRAILRDGEIQRYEEWTLERTTQVTGQPFEVTITVSFDFEGP
                     AAIQIDVTLEDETRRHGPQVLTDHRFQLAFTRAGELVAGTISDVEGDRFSLTLAFRNS
                     AALVQVRDRVDGGVVIGIRRDEAEPTTRIRVDITKDDEFEVIGESSDPINVTVGRDDR
                     QNRQIVVSGEIQASSTEPTEQPDVVSLDATYKEILPESWVVVERPDPDEEGTLETVIA
                     RAEQVREASRADYGITAKGTQITLSENWLYPNRDDFAVIRGTSVYAAPELLELADAPP
                     EVIDENGVVRVEPVAGGRIELDALYDGLEPGRWLFVTGERDDLPGVRASELVMLAGVE
                     QGVRLVSLTDENLPRVGEATALRLTGRGSTGPGGLDLTISARRAADGSVSGYAHLVGG
                     ATGEVVGIAPPDDERDFWCINVRRIDTGPNSGDQRINWYVRDAGDGITSFDEISYITS
                     IGGDCESLSAPTGSWLRLVKGDFHVSAEEPTGEKVHSTIILAGDLAYPYKRETVRIHG
                     NVVNATHGETRPEVLG"
     gene            complement(2963..>3608)
                     /locus_tag="VHM69_08975"
     CDS             complement(2963..>3608)
                     /locus_tag="VHM69_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740566.1"
                     /translation="SAERDEPPKRLAALVRESLPSLNEEHDRAVARDYERLAPWTGDI
                     VAALDRSLGMLPGHMTDAPEGDLLLQEIEEYLRPSRRIGHDLLVTQAQYVPLDIMLEV
                     CVLPSFLRGHVKAALLDHFSNRTLPDGRRGFFHPDNLSFGDGIFLSRLIATAQAVPGV
                     ESVVVTRLQRLFETPNREIEAGVLSLSPFEIARLDNDPSSPENGQFRLEMRGGR"
BASE COUNT          523 a         1176 c         1209 g          700 t
ORIGIN      
        1 ctgccgagca cctcgggacg cgtctcgccg tgcgtggcgt tcaccacgtt gccgtggatc
       61 ctgacggtct cccgcttgta gggataggcc agatcgcctg cgaggatgat ggtgctgtgg
      121 accttctcgc cggtcggctc ctccgcggat acatggaagt cgcccttgac cagtcgcagc
      181 caggaccccg tcggtgcgga gagactttcg cagtcccccc cgatgctggt gatgtaggag
      241 atctcgtcga aggaagttat cccgtccccg gcgtcgcgga cgtaccagtt tatccgctgg
      301 tcgcccgagt tcggtcccgt gtctatccgc ctgacgttta tacaccagaa gtccctctca
      361 tcgtcgggag gggcgatccc gacgacctca ccggtagcgc caccaacgag gtgcgcgtag
      421 cccgatactg agccgtcggc ggcgcggcgg gccgatatcg tcaggtcgag tcctcccggc
      481 cccgtgcttc ccctccccgt gagcctgagc gcggtggcct cgccgacgcg cggcaggttc
      541 tcgtcagtga ggcttaccag acgcaccccc tgctcgacgc cggcaagcat cacgagctcg
      601 ctcgcacgta cgccggggag gtcgtcgcgc tctccggtaa cgaagagcca gcgtcccggc
      661 tccaggccgt cgtagagggc gtcgagctct atccgcccgc cagcgacggg ttccaccctt
      721 acaacgccgt tctcatcgat cacctcgggt ggcgcgtcgg caagctcgag tagctccggc
      781 gcggcgtaga cggaggtgcc ccggatcacg gcgaagtcgt cccggttcgg gtagagccag
      841 ttctcggata gtgtgatctg cgttcccttg gccgtgatgc cgtagtcggc cctggaggcc
      901 tcgcgcacct gctcggccct ggcgatcacc gtctccaatg tgccctcctc gtcagggtcg
      961 ggcctctcca ccacgaccca gctctcaggg aggatctcct tgtaggtggc gtcgagcgag
     1021 acgacgtccg gctgctctgt tggttcggtc gagctcgcct gtatctcacc gctcaccacg
     1081 atctgcctgt tctggcggtc gtcgcggcct accgtgacgt tgatggggtc gctgctctcg
     1141 ccgattactt cgaactcgtc gtctttggtt atgtccaccc ttattcgcgt cgttggctca
     1201 gcctcgtcgc ggcgtatgcc tattaccacg cccccatcca ccctgtccct tacctggacg
     1261 agtgcggcgc tattcctgaa cgccagcgtc aggctgaagc ggtcaccctc cacgtcggat
     1321 atggttcctg cgacgagctc tccagctcgt gtgaacgcga gctggaacct gtggtccgtc
     1381 aggacctgcg gaccgtgcct gcgggtctca tcctcgaggg tgacgtctat ctggatcgcg
     1441 gccggtccct cgaaatcgaa gctaaccgtg atggtgactt cgaatggctg cccggtaacc
     1501 tgcgtggttc tttccagcgt ccattcttcg tacctctgga tctcgccgtc tcgcaggatg
     1561 gctctgggcg gagcgttgtg gccgaatggc gccgctgtga cgcgcagcgc gtagatgctc
     1621 accgggctcg gttcggtgcc ggtctgttcc cacgcctgat agagcgcgcg gcccaggtcc
     1681 ggcctcagcg cggagagcaa ctggggtacg aggtcggagt cggttccgaa gctcttcgcg
     1741 aggctcctgg atagtttcgc cgcgcttaca ggctgcacgg agggtggctt gccaaggcga
     1801 tccaacaacc catccaacgt cgccgatggc gcattgcctt ccccaagacg gccgttcacc
     1861 tccgcccggt tgctgaacgc gctgacggcg ccaccggatg cgcgggccac ccccagccgg
     1921 ctacttgcca actcgctctc gatcctctgc agctccccct caattcgttc gagccagggg
     1981 gtgagcctgg tgtagttgcg ctccctggct gtgctcaggt cctggcgcac gcccggcagc
     2041 accgtttcgc gcagggagtc cgcgggcgct tccggcggcg tctcgggggt caggcgggtc
     2101 tgcaagtctt ccaggaggcc aaccacgcgc cgggtcgtct cgctctccgg agagaccctg
     2161 tgcgcctcaa gatcgagtaa gccttccacc ctctgcctga tgtcctcgat ccctggcctc
     2221 gttgagatca cggtcgacgg gcgtggcagc gtgaacgtca caagggtgcg gtctctctcg
     2281 gggtcaggct ctaccgtccg caccttgatg agccgccggg ccaggccgga ttcgacgagc
     2341 agcgggtcgt tcggcccgag gttggttgag acgcccttca ggtagaggcg cccgcgcggg
     2401 gtgtcgtccc ccggaggagt tataggtcgc agggactggg ggcggctgag acgcggcgtg
     2461 aggacgttcc attcggagcg ggcctctagc gcttcggagg tctcgaagaa ctggggcagc
     2521 tcgccgggac cgggcaggct ctgggcgcgg gtgccggcgg gtatctcggt cctgtactcg
     2581 tcttcgaggg taagggccag gtagacgctc gcggctacgc cggggcgcgg gacgtaaccg
     2641 acgaggcggg caagctccag gatggagcgg cgctctgtgg ccgtacgcag gtaaccctcg
     2701 ttggctattc gctcctggta gaaggtgagt acgtcggcaa cggtggccca ggcgtcgagg
     2761 agcgcgatgc tcgggtcgtt cgccgcgcgg gtggtcagcc ctccaagggc agggtactct
     2821 gcggaggaga gacgggcgat catggtctcg aggaaggtgg cgtgggtgcc tacgcgatag
     2881 gagagcgcct ccaggccggg cctgttggcc gtcgagagcg gggtcacggc ttcgacgcct
     2941 tcgcagcagc cgcaggtctc gttcatcgtc cacctcgcat ctcgagcctg aattggccgt
     3001 tttcgggcga gctcgggtcg ttgtcaagac gcgcgatctc gaacgggctg agggacagta
     3061 cgccggcctc gatctcgcgg tttggcgtct cgaagaggcg ctgaagcctg gtgaccacga
     3121 cgctctccac ccccggcacg gcctgggctg ttgctatgag ccggctcagg aagatgccgt
     3181 cgccgaagct caggttgtcg ggatggaaga agccgcgtcg gccgtctggc agcgtgcggt
     3241 tgctgaagtg gtcgagcagg gcagctttga cgtggccgcg caggaagctg gggagcacgc
     3301 acacctcaag catgatatcc agaggaacgt actgagcctg tgtgacgagc agatcgtggc
     3361 cgatgcgcct gctagggcgg aggtactcct cgatctcctg cagcaacagg tcgccttctg
     3421 gagcgtcggt catgtgccct ggcagcatgc ccaacgacct gtccagcgcg gccactatgt
     3481 cgcccgtcca cggcgcaagc ctttcgtagt ccctggcgac ggcgcggtcg tgctcctcgt
     3541 tcagcgacgg tagactctca cgcaccagcg cggcgaggcg tttgggtggc tcgtcgcgct
     3601 ccgcgctc
//
LOCUS       DATAIR010000367         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_438847, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000367 DATAIR010000000
VERSION     DATAIR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438847"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHM69_08980"
     CDS             <1..334
                     /locus_tag="VHM69_08980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2740567.1"
                     /translation="YSHAVVSGGFVFISGQGPVDPETGTMPDAFSDQVRQTLENVRTI
                     LEAAGSSLDDVVKVNAYVTDLTRFQEFNEVYKEFFTHDPPARTTVGSSLLGFLVEIDC
                     IAAVPEAG"
     gene            complement(414..1628)
                     /locus_tag="VHM69_08985"
     CDS             complement(414..1628)
                     /locus_tag="VHM69_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740568.1"
                     /translation="MLAFATDLQDEGLDVVLDNVQERAGLGGLTLAAAYHDARDLFPH
                     NPVHKVRYLEGGAVFFQPDESRYEGLQLRPHVAGLARTTDPLEDLCAAADGRGMDANA
                     WVVFLHSDRLGLAHPGCATQNAFGDRYLTDLCPSNPEVRGYARALASDVARYDVSTVF
                     AESLHFHGLEHGYHHERYFEEIGAVGNYLLGLCFCVHCLEAAARSGIDAETVHRSVRD
                     ELERRLSGESEDDHSEGVSREHLADFGGGQLLGYIEARIGTVTSLAAEVSAAVADEGA
                     RFVFMDLSGAEKGFATGQPTGEAAPTIGWKTGVDVAALAEACDNVEVTGYAADPGRLR
                     FDLDAYHAVLADNARPGLLLRPMPPDCRSADNLAEKVAIAREHGLERLDFYHYGFCRL
                     RSLDWIRESRTA"
     gene            complement(1744..2217)
                     /locus_tag="VHM69_08990"
     CDS             complement(1744..2217)
                     /locus_tag="VHM69_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HEX2740569.1"
                     /translation="MDQDAIIQYVTETFADIEVLRPTDGPGAGDTFLYHDPMHNLDPT
                     RQFPFATIVTKDYGDFDEASDLNRPDVFRLNIGVGRDAFRALFGPPPGEYSTESTDYD
                     FAALDRLMPHPAYARQSWVCVLNPSPETFEAVKPLLAEAYSRVATRHARGQTNRD"
     gene            complement(2420..3226)
                     /locus_tag="VHM69_08995"
     CDS             complement(2420..3226)
                     /locus_tag="VHM69_08995"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2740570.1"
                     /translation="MRFQGRVTVITGGGSGLGRVLAHRFAEEGAAVVVADVVGHSATA
                     VADEISEAGGRSLAHTADVTKAGDVEAMVEAARKAFGPVEILVNNAAKATDADFLDLS
                     EETWEQDVAITLKGPFLCSQAVLPDMTENRSGVILNIASVNALTYFGNEAYSAAKAGV
                     LSLTRSLAVRYGPSGVRVNAIAPGTLRTPAWEQRRQKDPEVFERVSRWYPLGRIGEPE
                     DVAGAALFLASDEAAWISGAVLPVDGGLTAGNLQMAQEIVIGSEDNTTSQ"
     gene            3345..>3850
                     /locus_tag="VHM69_09000"
     CDS             3345..>3850
                     /locus_tag="VHM69_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2740571.1"
                     /translation="MNAVGVDVGGTKIAAGVVSPEGELLNEVRYPTENAREPLLSTIA
                     GAIMEVQRGYEIGGVCLAAPGFILASENKIMSAANLEAIEGIPLKEELGSRTGLRVTV
                     ENDANAAAWGEFRFGAGEDVDNLVFVTLGTGVGGGIISQGVLLRGARGMGGELGHITI
                     QPAGPRCG"
BASE COUNT          696 a         1199 c         1231 g          724 t
ORIGIN      
        1 gtactcacac gccgtggtat ccggcggctt cgtgttcatc tcggggcagg gtcccgtaga
       61 ccccgagacc ggcactatgc ccgacgcctt ctccgaccag gttcgccaga cgctagagaa
      121 cgtgcggacg atcctggagg ccgcaggctc cagcctcgat gatgtcgtca aggtcaacgc
      181 gtacgtgacg gacctcacgc ggtttcagga gttcaacgag gtctacaaag aattcttcac
      241 ccacgaccct ccggcccgta ccaccgtggg ctcctccctc ctcgggttcc tcgtggagat
      301 cgactgcata gccgcggttc cagaagccgg ctaacgactc ctgttcaaac agcggcgtcg
      361 gtggaggttg aacatcgccc cgatccaccc ctatccctga aacctgaaaa ctatcaggct
      421 gtacgagact ccctgatcca gtccagagag cgcagtctac agaacccgta atggtagaaa
      481 tcgaggcgct ccagaccgtg ctcgcgggca atggctactt tctcggcgag gttgtccgcc
      541 gatcggcaat cggggggcat gggacgaagc agcaggcctg gcctggcatt gtccgcgagc
      601 acggcatgat aggcgtcgag atcgaagcgt agccgtccag gatcggcggc gtagccggtg
      661 acttccacgt tgtcgcatgc ctctgccagg gcggcgacat ccacgcccgt cttccacccg
      721 atcgtgggcg cggcctctcc cgtgggttgc cccgtggcga agcctttctc cgccccagag
      781 agatccatga agacgaacct ggcgccctca tcagcgacgg ccgcagacac ttcggccgcg
      841 agcgatgtga ccgtccctat ccgcgcttcg atgtatccga ggagttgccc gcctccgaag
      901 tctgcgaggt gttcgcggga gactccttcg gagtggtcgt cctcgctctc gccggataac
      961 cggcgctcca actcgtcgcg gacggagcga tggaccgttt cagcgtcgat gccagagcgg
     1021 gctgccgcct ccagacagtg gacgcagaaa cacagaccga gcaggtagtt tcccactgcc
     1081 ccgatctcct cgaagtagcg ctcgtgatgg tagccgtgct ccagcccgtg aaaatggagc
     1141 gactcggcga agactgtcga gacgtcgtag cgcgcgacgt cggacgcgag cgcccgcgca
     1201 tagcctcgca cctccgggtt ggatggacac aggtcggtga ggtatcggtc cccgaacgcg
     1261 ttctgtgtgg cgcagccggg atgcgcgagg ccgagcctgt cgctgtgcag gaagacgacc
     1321 cacgcgtttg cgtccattcc ccgcccgtcg gcggccgcgc agaggtcttc cagaggatcg
     1381 gtcgtgcggg caagcccggc gacgtgcggc ctgagttgca ggccctcgta gcgtgattcg
     1441 tccggctgga aaaagacggc gccaccctcc aggtaacgga ccttgtggac gggattgtgg
     1501 gggaagaggt ccctggcgtc gtggtacgct gcagcgagcg tcagcccgcc caggccggct
     1561 cgctcctgga cgttgtcgag gaccacgtcg agtccctcgt cctgaaggtc ggtggcgaac
     1621 gcgagcatcg acgtgtccat gcggccagac tatacagccg ggctcttggg atcaaggccc
     1681 gatacgagca ccttcacatg agaagccgtc gcctgtcgcc tgcagctatg gagcggcgac
     1741 tgctcagtcg cgattggtct ggccgcgggc gtgccgggtg gcgactctgg agtaggcttc
     1801 ggccagcaag ggcttgactg cttcgaaggt ctccggactt gggttgagca cacacaccca
     1861 cgattgccgc gcgtacgcag gatgtggcat cagccggtcc agggctgcga agtcatagtc
     1921 ggtgctctca gtactgtact caccgggtgg agggccaaag agagcacgaa aggcgtccct
     1981 gccaacgccg atgtttagcc gaaacacgtc agggcgattc agatctgagg cctcgtcaaa
     2041 gtcgccgtag tctttggtga cgatcgtggc gaatggaaac tgacgcgtcg gatcgaggtt
     2101 gtgcatgggg tcatggtaaa ggaaggtgtc gcctgcaccg ggaccatcgg ttggccttag
     2161 tacctcgata tccgcgaacg tttccgtaac gtattggatg atggcgtctt ggtccacaag
     2221 atccaaaggt tactccgtgg tgccgccgtg tccccgaccc gctggctcgt ccccgatttt
     2281 cccactcccg ctgcccgtgt gcaacgacta cgcagttcag agaacacgca tgactcgaag
     2341 ctgcttgagc ctctattgga tacggtccaa gctggtccgt ggtgagccgg acccccactg
     2401 aaatcagctt caaagtccgc tactgcgagg tagtgttgtc ctcggaccct atgacgatct
     2461 cctgcgccat ctgaaggttg cctgcggtga gtcccccgtc gacggggagg acggcgcccg
     2521 agatccaggc cgcctcatca gatgcgagga agagggcggc tccggcgacg tcctcaggct
     2581 caccgatgcg gccgagggga taccatcggc tcacgcgctc gaagacttcg gggtccttct
     2641 gacgacgttg ttcccaggcc ggcgttctga gtgtgcctgg ggcgatggcg ttcacgcgta
     2701 cgccggaagg tccgtagcgt acagccaggc tcctggtcag gctgaggaca cctgccttcg
     2761 ccgcgctgta tgcttcgttg ccgaagtacg tgagggcgtt taccgaggct atgttgagta
     2821 tgactccaga cctgttctcc gtcatgtccg gaagcacggc ttgtgagcag aggaacggtc
     2881 ccttcaacgt gatcgccacg tcttgttccc aggtctcctc gctgaggtcg aggaaatccg
     2941 cgtctgtagc tttcgccgcg ttgtttacga gtatctctac cggtccgaaa gccttcctgg
     3001 cagcctctac catggcctcg acatcgccag ctttggttac gtccgccgtg tgtgccaggg
     3061 acctgccgcc tgcctcggag atctcgtctg ctaccgccgt cgcgctgtgg ccaaccacgt
     3121 cggccacgac gactgcggcg ccctcctctg caaaacgatg tgccagcacc cgcccgagtc
     3181 ccgagccgcc acccgtgatg acggtcaccc gaccttggaa ccgcatcttc accccctacg
     3241 ttcgtggctg gcgtcaacgc ccggccttgt gggcaactag aatacagtaa acgacaatct
     3301 cccgagctta cgcggcactt cttcgggacg ggtgaggaga ggctatgaat gccgtagggg
     3361 tggacgtagg aggaaccaag atcgccgccg gggtcgtttc gcccgagggc gagctactga
     3421 acgaggtgag atacccgacg gagaacgcca gggaaccgtt gctctcgacc atcgccgggg
     3481 cgatcatgga ggtccagcga ggctacgaga taggtggcgt ttgcctggcg gcccctggct
     3541 tcatcctcgc cagcgaaaac aagatcatga gcgcggccaa cctcgaagct atcgagggca
     3601 tacccttgaa ggaggaactg ggcagtcgaa cgggactacg ggttaccgtg gagaacgacg
     3661 ccaacgccgc ggcctggggt gagttccgat tcggtgccgg agaggacgtc gataacctgg
     3721 tcttcgtgac gcttgggacg ggggtgggcg gcgggatcat ctcgcaaggc gttctactca
     3781 ggggcgcccg cggtatgggc ggggagttgg ggcacataac catccagccc gccggtcctc
     3841 gctgcggatg
//
LOCUS       DATAIR010000368         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_24049, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000368 DATAIR010000000
VERSION     DATAIR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24049"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM69_09005"
     CDS             <1..235
                     /locus_tag="VHM69_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX2740572.1"
                     /translation="PVVDLLRASREVSASRPGSARRALGEHEGILRRIEDNDAEGARQ
                     EMPVHLENTAEDIEAALDEGDIETMKDWDKEGI"
     gene            232..1173
                     /locus_tag="VHM69_09010"
     CDS             232..1173
                     /locus_tag="VHM69_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX2740573.1"
                     /translation="MKANDLLAGLGLPGRDLVDLPESEKRFPDGAQYRVEIPSVEGPR
                     VLEAVLDEAEKRGVQLHRISQGSGIMLQTDSEIREMCAMAREAGLELSLFVGPRASWE
                     TGATVISGAGKTLGAQHRGQDQLAYALEDVLRGVELGLRGVLVADAGLLWVLRDLKKN
                     GDLPEDLVVKVSVQLSAANAAAIKAMENLGADTYNVPTDLSLAQLAAMRAVSDVPLDV
                     YMEVPDDFGGFVRHYEIPDLVRVASPVFVKFGLRNAPNIYPSGTHLEPTAIALGRERV
                     RRAEIGLSMLERYYSGAETTERGATFPGIPVEAKERV"
     gene            1170..1454
                     /locus_tag="VHM69_09015"
     CDS             1170..1454
                     /locus_tag="VHM69_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740574.1"
                     /translation="MKITDVETFVVGTEWRNLTIVRLHTDDGLTGLGEARMVNHTDAL
                     VGYLKEAAPRHIVGHDPFRIEELVKKLGRDDFSRAGEVMSSGIAAFETAC"
BASE COUNT          312 a          464 c          580 g          267 t
ORIGIN      
        1 acccgtggtt gacctgctgc gggccagccg cgaggtgagc gcctcgcggc caggcagcgc
       61 ccggcgcgcc ctcggcgaac acgaagggat actgcgccgc atcgaggaca acgatgctga
      121 aggagcgcgg caagagatgc ccgtccacct cgagaacacg gctgaggata tcgaggcggc
      181 gctcgatgaa ggtgacatcg aaacgatgaa agactgggat aaggagggga tatgaaagcg
      241 aacgacctgt tggctggcct tggcctgcct ggccgggatc tcgtggatct tcccgaatcg
      301 gagaaacgtt ttcccgatgg ggcgcagtac cgggtagaga taccaagcgt cgaggggccg
      361 cgggttctgg aggcggtgct ggatgaggcg gagaagcgcg gcgtgcagtt gcacagaatc
      421 tctcagggat caggcatcat gctccagacg gactccgaga tccgggagat gtgcgcgatg
      481 gcgcgggagg ctggcctgga actctcgcta ttcgtcgggc cgcgcgcctc atgggagacc
      541 ggggcgacag tgatctccgg cgcgggcaag acgctcggcg cgcagcatcg cggccaggat
      601 caactcgcat acgccctcga agatgtgctg cgcggcgtgg aactgggatt acgcggcgtg
      661 ctcgtcgccg acgctggcct cctctgggtc ctgcgggatc tgaagaagaa cggagatctg
      721 ccggaagacc tcgtggtcaa ggtgtcggtg cagctatctg cagcgaacgc agccgcgatc
      781 aaggcgatgg agaacctcgg ggctgacacc tacaacgtgc cgacggatct ctccctcgcg
      841 caactcgcgg cgatgcgggc ggtctcggat gtcccgctcg acgtgtacat ggaggttcct
      901 gacgatttcg gtgggttcgt gcggcactat gagatcccgg atctggtacg ggttgcgtcg
      961 cctgtgttcg tgaagttcgg gttgcggaac gcgccgaaca tctatccgag cgggacgcac
     1021 cttgagccga cggctatcgc gctcggccgc gagcgggtac ggcgcgcgga gatcggtctt
     1081 tcaatgttgg aacgctacta ttccggcgcg gagacgacag agcgtggcgc gacctttcca
     1141 ggtatccccg tcgaggcaaa ggagagggta tgaagatcac ggacgtagag accttcgtcg
     1201 tcggcacgga gtggcgcaac ctgaccatcg tgcgcctgca caccgacgac gggcttacgg
     1261 gcctcggcga agcgaggatg gtgaaccaca ccgacgcgct ggtcggctac ctgaaagagg
     1321 cggcaccgcg tcacatcgtc ggccatgatc cgttccgcat cgaggagttg gtgaagaagc
     1381 tcggtcgcga cgatttttcg cgggctggag aggtgatgag cagcggcatc gccgccttcg
     1441 agaccgcgtg ctgagacatc gtggggaagg ctctcgacca gccggtctac aacctgctcg
     1501 gcggtccggt gcgcgaccgc gtgaaagcct acgccaacgg ctggtacagg gtcgagcgcg
     1561 agcccgaaga gttccacgag gccgcgaaga aggtggtcga gcgtggctac aaggccctca
     1621 agc
//
LOCUS       DATAIR010000369         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_393225, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000369 DATAIR010000000
VERSION     DATAIR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393225"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHM69_09020"
     CDS             complement(<1..252)
                     /locus_tag="VHM69_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740575.1"
                     /translation="MLFRIGAAAFILIAMVLAGCAHSDEEVSIEPLAPAAGRVDPGLG
                     PGTRALSSGPGYKGSPSWSPGGGRIAFTVDGYVVDRPAGP"
     gene            complement(242..>1988)
                     /locus_tag="VHM69_09025"
     CDS             complement(242..>1988)
                     /locus_tag="VHM69_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2740576.1"
                     /translation="DRLVAVENTLLKQRSQTANAGAQDFLEGLRDPENMTLPPSKTYA
                     EELVRSVADPTGLEVLYVGPEGKPLAARDGLGNPINERKAYEDHALDEGELEEIAVSQ
                     RGAGRLVWCEKRSGFVAVWPLTASDGTVKGVMIYDVPRDELGETLAYLRYGILGAILT
                     SILLAGAASLLLTRQVTRPLSETRDAAIRVASGDYAATVPVKSRDELGEMARAFNYMA
                     EEIEHYVGEIQEQKSRLEAVLEASPEAVVATGPDERVTMVNPAAARMLGIRTSDRGRT
                     MEEIGAPRGILRCLREAAANGVAVREVEVGEKAYWAYAAQMNRSGVARNGGGNAGIIL
                     AVRDITEHRSLERAKTAFVSDFSHELRTPLTTIQSAVGLLERARERLDPLEHRALELA
                     DQELKRIRGMIEELLTLAQMDSWQYQLEVGPANLDTVVQTAIESVEAKAQRFGIGIYF
                     DDAGEHRCICDVQKLYQVFLNLLDNAIKYSDSGDRVDIEIEEDVSTLNVRIRDTGVGI
                     PEEDLNQLFERFYRVDKDRSRATGGSGLGLAISKQIVEMHGGSIYVESEVGVGSVFEV
                     RVPKAFISRSVSHAL"
BASE COUNT          299 a          675 c          621 g          393 t
ORIGIN      
        1 cgggccggcc ggcctgtcga ccacgtagcc gtcgacggtg aacgcgatcc tgccgccccc
       61 ggggctccag ctcggcgagc ctttgtaccc gggacccgag ctcagggccc gcgtcccagg
      121 gccgagtcca gggtcgaccc tgccggccgc cggggccagg ggctcgatgg agacctcctc
      181 atcggaatgg gcgcatcccg cgaggaccat cgctatcaag atgaacgcgg cggcccctat
      241 cctaaagagc atggcttacg gagcgggaga tgaacgcctt tggcaccctg acctcgaaga
      301 cggagccgac accgacctcg ctctcgacgt agatactgcc gccgtgcatc tccactatct
      361 gcttgcttat ggcgagaccc aggccgctcc ctcccgtcgc gcgggagcgg tccttgtcca
      421 ccctgtagaa gcgctcgaag agctggttca ggtcctcttc ggggatgccg acacccgtat
      481 ccctgatgcg tacgttcagc gtcgagacgt cctcctctat ctcgatgtcg accctgtcgc
      541 cggaatctga gtacttgatc gcgttgtcga gcaggttgag gaagacctgg tagagcttct
      601 ggacgtcgca gatgcaccgg tgctcgcccg cgtcgtcgaa gtatattccg atgccgaacc
      661 tctgtgcctt ggcttccact gactctatgg cagtctggac cacggtatcc aggttggccg
      721 gcccgacctc cagctggtac tgccaggaat ccatctgggc gagggtgagc agctcctcga
      781 tcatgccgcg tattcgcttg agttcctgat ccgccagctc aagcgccctg tgctccaggg
      841 ggtcgagcct ctctctcgcc ctctccagaa ggccaaccgc gctctggatg gttgtcaggg
      901 gggtcctcaa ctcgtgggag aagtcggaga cgaaggccgt cttcgccctc tcgagcgagc
      961 ggtgctcggt tatgtccctg acggcgagga tgatgccggc gttcccgccg ccgttgcgtg
     1021 cgacgccgga cctgttcatc tgggcggcgt aagcccagta ggccttctcg cccacctcga
     1081 cctcccttac ggcgacgccg ttggcggccg cttcgcgcag gcacctgaga atcccgcgtg
     1141 gagccccgat ctcctccatc gtgcgccccc ggtctgatgt cctgatgccg agcatccttg
     1201 ccgccgcggg gttgaccatc gttacccgct catcggggcc cgtcgcgacg accgcctcgg
     1261 gagaggcttc gagaacggcc tcgaggcggc tcttttgctc ctgaatctcc ccgacgtagt
     1321 gctctatttc ctcggccatg tagttgaagg ccctggccat ctctccgagc tcgtccctgc
     1381 tcttgacagg cacggtcgcc gcgtagtccc cggaggcgac ccttatggct gcgtcacggg
     1441 tctcggagag aggacgcgtg acctggcgcg tgagcagcag gctcgccgca cctgcaagga
     1501 ggatgctggt taggatggcc ccgaggatcc cgtagcgcag gtaagcgagc gtctcaccga
     1561 gctcgtcgcg gggaacgtcg tagatcatga cccctttcac ggtcccgtcg gaggccgtga
     1621 gcggccacac cgcgacgaaa cccgagcgtt tctcgcacca cacgagcctg cccgcgcccc
     1681 gctgcgatac ggcgatttct tcgagctcgc cttcgtcgag tgcgtgatcc tcgtatgctt
     1741 tgcgctcatt tatgggattg ccgagaccgt cgcgcgcggc cagaggttta ccttctggcc
     1801 ccacgtagag cacctcgaga cccgtcgggt cggcgaccga ccggacgagc tcctcggcgt
     1861 aggtcttcga tggtgggagg gtcatgtttt ccgggtcgcg caggccttcg aggaagtcct
     1921 gcgctcccgc gttcgccgtc tgggagcgtt gcttgagcag cgtgttctcc acggctacca
     1981 gcctgtcc
//
LOCUS       DATAIR010000370         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_57080, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000370 DATAIR010000000
VERSION     DATAIR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57080"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VHM69_09030"
     CDS             complement(<1..1017)
                     /locus_tag="VHM69_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2740577.1"
                     /translation="MHTTDAREGAVSETRTVASVIGGRASDGAPGGRMTSTNPARRSE
                     KVAEVLLGDAGTFVEACRAAREAQREWASVPAPIRSQVIKKIGRIVENNKEALARLVT
                     REIGKPYAESLGEVQEIIDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRVPVGVATII
                     TAGNFPVAVPSWYLIPALTCGNAVVWKPAEYSPAIGDALARLFMAGGLPDGVLNVVQA
                     EGPATFEGLEIALDEGLVDKVGFTGSSEVGKRIGELCGRHMQSPCLELGGKNPLVVMP
                     DADLDLAAEGALFSGFGTAGQRCTSLGTVIVHESVHDDFLARFTRAVEEAPIGDPTQD
                     VLYGP"
     gene            1147..2058
                     /locus_tag="VHM69_09035"
     CDS             1147..2058
                     /locus_tag="VHM69_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2740578.1"
                     /translation="MGIVDKAIANSVPVIPRPIVKRISSRYIAGDTLEDAVSTVRDLN
                     RRGCVATIDLLGESTESKADAAATLENHKKVFDAIDEHSLNSGISVKLTGLGLTLNEE
                     LCRANLEEIVEYARGRFVRVDMEDSPYTEATLNIVHDTYRRHENIGAVLQAYMRRSIE
                     DVQRIIGAGFSVRLCKGIYDEPRSIAYKDFDTVRQNYIFLLDELLKGGVYVGIATHDE
                     FLIWHALRLIHQLEVPEDRYEFQMLLGVDEELRNILVGAGHKLRVYVPFGEDWYEYST
                     RRLKENPKIAGYVARDVIGGITGTIRR"
     gene            2066..>2709
                     /locus_tag="VHM69_09040"
     CDS             2066..>2709
                     /locus_tag="VHM69_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2740579.1"
                     /translation="MGLIPYKNEVYLDWSDEKNVESMRAALKDVGSLLDRSYPAIIGG
                     KRVETDGEIASVNPANPSQVVGRTARATEREADIALDMATAAFEHWSRTSPEARARIL
                     LRASAIMKRRRFELLAWEAYEGGKPWAEADAQVAEAIDFLEYYAREMLRLKDGVEIYA
                     IPGEESRYTYQPMGVGVIIAPWNFPTAILTGMSSAAIVTGNTIIMKPSEFTPVIG"
BASE COUNT          528 a          846 c          861 g          474 t
ORIGIN      
        1 cgggccgtag agtacgtcct gcgtcgggtc ccctatgggg gcttcctcga cggcgcgggt
       61 gaacctggcg aggaaatcgt cgtggacgga ctcgtgcacg atcaccgtgc cgagggaggt
      121 gcagcgctgg cccgccgtgc cgaagcccga gaacagggcg ccttcggcgg cgaggtcgag
      181 gtccgcgtcg ggcatgacga cgagggggtt ctttcctccg agctccaggc atggggactg
      241 catgtgcctg ccgcagagct cccctattct cttcccgacc tcggaggagc ctgtgaagcc
      301 gaccttgtca acgagacctt catcgagggc gatctcaagc ccctcgaagg tcgcgggccc
      361 ctccgcctgc acgacgttga ggacgccgtc ggggaggccg ccagccatga acagccgcgc
      421 cagggcgtct ccgatggccg gggagtactc ggcgggtttc cagaccacgg cgttgccgca
      481 ggtgagcgcc gggatcagat accaagaagg tacggcgaca gggaagttgc ctgcggtgat
      541 aatggtagcc acgcctacag gcacgcggaa ggtgaagagt tgtttgtctg gcatctccga
      601 ggggaccgtc tgtccgtata gtcttcggcc ctctccggta aagaagtcgc aggtgtctat
      661 gatttcctgc acctcgccga gggattccgc gtacggcttg ccgatctcac gcgtaacgag
      721 gcgcgcgagc gcctccttgt tgttctcgac tatgcgcccg atcttcttta ttacctgcga
      781 gcggatcggg gccggcaccg aggcccattc ccgctgcgcc tcccttgccg ccctgcaagc
      841 ctccacgaac gtccccgcgt cgccaagaag cacctcggcc accttctcgc tcctgcgagc
      901 cgggttcgtc gaggtcatgc gcccgccggg agcgccgtcg gatgcgcgtc cccctatcac
      961 ggaggcgacg gtacgtgtct ctgaaactgc cccctcacga gcgtcggtgg tatgcattgt
     1021 ctacctccag actttcacta tttccctatc gtgccaccat cgtacaccaa agcttcggga
     1081 cgctccgtga agtagaatta ggggtggatc tggcgtaata agagccataa tgcggggtac
     1141 ttagacatgg ggatagtgga caaggccata gcgaactcgg tgcccgtcat cccgcgtccc
     1201 atcgtaaaga ggatctccag ccgctacatc gccggcgaca cgctcgagga tgccgtctcc
     1261 accgtcaggg acctcaacag gcgcggctgt gtcgcgacca tcgacctcct cggcgagagc
     1321 accgagagca aggccgacgc cgcagcgacg ctcgagaacc acaagaaggt cttcgacgcc
     1381 atcgacgaac attccctgaa tagcggcatc tccgtcaagc tcacggggct cgggctgact
     1441 ttgaacgagg agctgtgcag ggcgaacctg gaggagatcg tcgagtacgc gaggggccgc
     1501 ttcgttcggg tggacatgga ggactccccg tacacggaag ccaccttgaa catagtccac
     1561 gacacgtacc ggcgacacga gaacatcggc gccgtgttgc aggcttacat gcgccgcagc
     1621 atcgaggacg tgcagcgaat catcggggcg gggttctccg tgcgtctgtg caagggcatc
     1681 tacgacgagc cgaggtctat cgcgtacaaa gacttcgaca ccgtccgcca gaactacatc
     1741 tttctgctcg atgagctgct caagggaggt gtctacgtcg gcatagcgac tcacgacgag
     1801 ttcctgatct ggcacgccct caggctcatc caccagctcg aagtccccga agaccgctac
     1861 gagttccaga tgctactggg cgtggacgag gaactcagga acatactcgt cggggccggg
     1921 cacaagctca gggtctacgt gcctttcggg gaggactggt acgagtactc tacccgtcgt
     1981 ctcaaagaga acccaaagat cgccggctac gtagcgaggg acgtgatcgg gggcataacc
     2041 ggcacgataa ggagatagga gcatcatggg actgattccg tacaagaacg aagtatatct
     2101 ggactggtcg gacgagaaga acgtcgagtc gatgcgggcc gccctgaaag atgtcggctc
     2161 cctgctggac aggagctacc ctgcgatcat cggcggcaag cgcgttgaga cggacggcga
     2221 gatcgcctct gtcaaccccg cgaacccctc gcaggtcgta ggccgcaccg cccgcgccac
     2281 ggagcgcgag gcagacatag cgcttgacat ggcgactgcg gcgttcgagc attggagccg
     2341 cacttcgccc gaggcgcggg cgcgcatact gctgcgggct tcggcgatca tgaagcgcag
     2401 gaggttcgag cttctcgcct gggaggccta cgagggcggc aagccgtggg ccgaggccga
     2461 cgcgcaggtc gccgaggcaa tagacttcct cgaatactac gcccgcgaga tgctgcgtct
     2521 caaggacggg gtggagatct acgcgatccc tggcgaggag agccgctaca cgtaccagcc
     2581 gatgggcgtc ggggtcatca tcgctccctg gaacttccct accgccatcc ttacggggat
     2641 gtccagcgcg gccatcgtca ctggcaacac catcatcatg aagccatccg agttcacgcc
     2701 ggtcatagg
//
LOCUS       DATAIR010000371         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_439323, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000371 DATAIR010000000
VERSION     DATAIR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439323"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VHM69_09045"
     CDS             complement(<1..996)
                     /locus_tag="VHM69_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740580.1"
                     /translation="MKRTTLLLPLLLPLLLCALGFARGSQTDVHEVVQKASFVPKLEE
                     YYSRPTVETTASYEGDKDIWRVVLTEQTSEKVVARFEVADDTGDVSGVEVSPNADEIK
                     YPQLSEEKAVKIAAASPEVREELAKHGPHNTQAAYETGEWTVRFYVDETGAVGGRPTD
                     EGKEVATVGVDDSTWALNYVYTGDQVGWNMARGIPGAYGKQANYWWVWLPLALAFAAA
                     FWRTDRLFSLRNLDVVALLGFLVSHGFFREGIVYEAVILWYPPLIYLIARTLLMGFGI
                     GERVERTSNLPVWLLMTLAGLAGGLVLGLNVDSRVIDVGYAGVVGADRILDGTIPY"
     gene            complement(993..2144)
                     /locus_tag="VHM69_09050"
     CDS             complement(993..2144)
                     /locus_tag="VHM69_09050"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2740581.1"
                     /translation="MCLVLYTLSARLPPGLREGSNDLSIYRQAGEAILRGEIPYRDFF
                     IEYPPGSLPAFVPPALFSADRAGYISLFSAEMALVLAATLLLTALTARRRRGPRAWIL
                     PSVTFVAAGILLYPVALTRYDALVALTLAIAALCAAPGRHHVGLAYASLGVGTAAKLV
                     PALATVPLALIYRGARRGYIVFFAVLALSFVPFLLLGGLLKSFTYQAERGLQVESVAA
                     SVLMELGWVNDIVFEYGAFEVRGMGVGLASTLSLPITLVLLFVTGLVMHREHRRLGRL
                     GGEAFPRYAAALILAFMLGSKVLSPQYLIWLLPLVPLAAGGAYGLGVSTLFLAACFIT
                     TQVFPAHYGDLLELRYPGPELLLARNLLLAGLWVLLLFLPGKSPATSAP"
     gene            complement(2240..>2691)
                     /locus_tag="VHM69_09055"
     CDS             complement(2240..>2691)
                     /locus_tag="VHM69_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX2740582.1"
                     /translation="EMDEMRYRLERWVLLAVPHVEDPSRLRGLLHLATASETIANCAM
                     EMVWVVEKGEEVHPVLSAAVEESDEVVLKLTVSPGSPADGKTLGSLRIETETGMYVLA
                     VNRGGRWTYRPRDTYRLQGGDSLLVTGAPEGFEPLAELFGQELEGSP"
BASE COUNT          502 a          829 c          927 g          433 t
ORIGIN      
        1 gtagggtatt gtcccgtcca ggatgcggtc ggcgccaacg accccggcgt agccgacgtc
       61 tatgacgcgg gagtcgacgt tgaggccgag gacgagacca ccggcgagcc cggcgagcgt
      121 catcaggagc cagaccggca ggttgctcgt cctctccacc ctctcgccta tcccgaatcc
      181 catgagaagg gtgcgcgcga tgaggtagat gaggggcgga taccagagga tgacggcctc
      241 gtagacgatt ccctccctga agaaaccgtg cgagacgagg aacccgagca gggccacgac
      301 gtcgaggttt cgtagcgaga acaacctgtc cgtgcgccag aaagcagcgg cgaaggctag
      361 cgccagtggt aaccagaccc accagtagtt ggcctgcttg ccgtaggcgc ccggtatgcc
      421 gcgggccata ttccacccga cttgatcccc cgtgtacacg tagttgaggg cccaggtcga
      481 atcgtcgacc ccgacggtcg ccacctcctt gccctcgtcc gtcggacggc caccgaccgc
      541 ccctgtttcg tcgacgtaga aacgaaccgt ccactcgccc gtctcgtacg cggcttgcgt
      601 gttgtgagga ccgtgtttgg cgagctcttc cctgacctcg ggggaggcgg cggcgatctt
      661 gaccgctttc tcttccgaga gctgggggta tttgatctca tccgcgttcg gcgagacttc
      721 gaccccgctc acgtctcccg tatcatccgc gacctcgaaa cgggccacga ctttctccga
      781 ggtctgctcg gtgaggacga cccgccagat gtccttgtct ccctcgtagc tcgccgtcgt
      841 ctcgaccgtc ggtctggagt aatactcttc caacttgggg acgaagctcg ctttctggac
      901 gacctcatgt acgtccgtct gggagccccg cgcgaagcca agggcgcaga gcagcagcgg
      961 caacaggagc ggcagcagta acgtcgtgcg tttcatggcg cgctcgtggc tggactctta
     1021 ccgggcagga acagcagcag gacccagagg ccggcgagca gcaggttgcg ggcgaggagt
     1081 agttcgggcc ctgggtagcg aagttcgagc aggtcgccgt agtgtgcggg aaagacctgc
     1141 gttgtgatga agcaggcggc gaggaacagc gtcgagacgc cgagcccata cgcgcctccc
     1201 gcggccagtg gcacgagcgg gagaagccag atcaggtact gcggagagag gaccttggag
     1261 ccgagcatga acgcaagtat aagcgccgcg gcataccgcg gaaaagcttc tcctccgagc
     1321 cttccgaggc ggcgatgctc tctgtgcatc acaagtccgg tgacgaacag caaaacgagc
     1381 gtgatgggca ggctgagagt actagccagc ccgacgccca tgccacgcac ctcgaaggcg
     1441 ccgtactcga agacgatgtc gttgacccaa ccgagttcca tcaggaccga ggccgcgacg
     1501 ctctcgacct gcaatccccg ttcagcctgg tacgtgaagc tcttgagaag ccctccgagc
     1561 aggaggaagg gcacgaagga gagtgcgagt accgcgaaga agactatata gcctcgcctg
     1621 gcacccctgt agatgagtgc gagcggcacg gttgcgaggg cggggacgag cttggctgcg
     1681 gtgccgacgc cgagcgaggc gtaggcgagg ccgacgtggt gcctgcccgg cgcggcgcac
     1741 agggcggcga tggccagcgt gagggcgacg agtgcgtcgt agcgggtgag ggctacgggg
     1801 tagaggagga ttccggcagc cacgaatgtc accgagggca ggatccaggc tcgcggcccg
     1861 cgccgcctgc gggctgtgag cgcggtcagc aggagagtcg cggcgagcac gagcgccatc
     1921 tcggcggaga agaggcttat gtaaccggcc ctatccgcgc tgaagagggc aggcgggacg
     1981 aaggcgggta ggctgcccgg cgggtactcg ataaagaagt cccggtaagg gatctccccg
     2041 cgcaaaatcg cttctcctgc ctggcggtag atggagaggt cgttggaccc ctctctcagg
     2101 ccgggcggca gcctggcgga cagggtgtac agtaccaggc acatgaaggc tgcgagcagg
     2161 gcaagcggtg cccagaaaag tagcgcgtcc cgccgggcat ggccccggaa gatgtgtcgt
     2221 ctctgagggc tcttccggat tacggggagc cctccagctc ctgaccgaag agctcggcga
     2281 ggggttcgaa gccctccggt gcgccggtga cgagcagcga gtcgccgccc tgcagcctgt
     2341 acgtgtcgcg gggtctgtag gtccaccgcc cgcccctgtt cacagcgagg acgtacatcc
     2401 ctgtctcggt ctcgatcctt aaagacccga gcgtcttgcc gtcggcgggg gagcccgggg
     2461 agacggtgag cttcaggacg acctcgtctg actcttcgac ggcggcggag aggacgggat
     2521 ggacctcttc gcccttctcg acgacccaca ccatctccat cgcgcagttg gcgatggtct
     2581 ccgaggcggt ggcgaggtgc agcaggcccc tcaggcgaga ggggtcctcc acgtggggga
     2641 cggcgagaag gacccagcgt tcgaggcggt aacgcatctc gtccatctcg t
//
LOCUS       DATAIR010000372         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_301393, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000372 DATAIR010000000
VERSION     DATAIR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301393"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..643
                     /gene="aat"
                     /locus_tag="VHM69_09060"
     CDS             71..643
                     /gene="aat"
                     /locus_tag="VHM69_09060"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX2740583.1"
                     /translation="MKLTPEILEACYRAGAFPMADSHGRIEFYRSNPRSVLELEDLHV
                     SRSLARVIRKGNYEVRVDEDFEGVTRACADREETWINAEIIDAYVRLHRQGKAHSVEA
                     YKAGQLVGGLYGVSLGGAFMGESMFAHARDASKVCFVHLVERLRERDFSLLDCQIQNS
                     HLASLGAREIPEREYLRRLGEALSLDRSFV"
     gene            816..1700
                     /locus_tag="VHM69_09065"
     CDS             816..1700
                     /locus_tag="VHM69_09065"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2740584.1"
                     /translation="MDIRHIDIDFEPELERPVLIAGFTGWNDAADAASVAVGALASSW
                     EARRFGGFDGEEFFDFQTTRPQVKLVDGVTREIEWPENVLSATEPALEAAGGRGGILL
                     TGPEPNFRWRTFSAAVVELAKELDVELVVTMGALLADVPHSRPVAVAANSQDATLVES
                     LGLSASRYEGPTGITGVLHRLCADGGLPSVSFWASVPHYLPAVPSAPAALALLEKLSS
                     LLGMQVETADLQSTAKTYQEQVSVAVSQDSDLSSYVRMLEERFDSQADQGPRNLPSGD
                     ELAQELEGFLREQRNDEE"
     gene            complement(2120..2668)
                     /locus_tag="VHM69_09070"
     CDS             complement(2120..2668)
                     /locus_tag="VHM69_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX2740585.1"
                     /translation="MDSVEGTLLVLTLIVMALGLIGSVVPGLPGVTLIFLSALVYAII
                     TDFRSVGVAILVLLFVFAAIAFVADFVATSYGARRFGASNWGTVGGAIGGIAGALIGL
                     FFLGIGSIFGLILGTIAGVFLGEYLKRERQGTEEQSSSGSDWRRASRAAGGVFVGYLA
                     AAVVQGLLGLASVVVFVLALVY"
     gene            complement(2671..3528)
                     /gene="larE"
                     /locus_tag="VHM69_09075"
     CDS             complement(2671..3528)
                     /gene="larE"
                     /locus_tag="VHM69_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX2740586.1"
                     /translation="MNPVATDTLHKRLEELEEILAPHGSALVAFSGGVDSSLALAVAA
                     RALPKDRVLAVTSNNETYLPSELDLAREFAGSLGVEHLVVNTRELDDPNYASNPTNRC
                     YFCKSTLYSDLAQLADDRGYACVVDGANKDDEGDYRPGRKAARELGVVSVLSEAGMTK
                     AEVREMAKYLDLPTWDKPALACLSSRFPYGQEITPQKLSQVARAEEFLRREGFRQVRV
                     RHHGEIARLEVGPDELERAFAMREEISTELLDAGFLYVTLDLAGYKSGSLNAALKKGR
                     KKTLPVISS"
     gene            complement(3542..4114)
                     /locus_tag="VHM69_09080"
     CDS             complement(3542..4114)
                     /locus_tag="VHM69_09080"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX2740587.1"
                     /translation="MTRNDSLYSVPDGIPEPPDDGACGHLPGMRLHSVSLTSTDGDRV
                     DLSTLTGTTVVYCYPMTGRPDQDLPQGWDDIPGARGCTPQSCAFRDHHAELQALGARV
                     FGLSTQDSAYQREAAERLHLPFLLLSDERLEFAVSLDLPTFEAEGMTLIRRLTLVIED
                     GEIREVFYPVFPPGRNAEEVLGWLSERTAS"
BASE COUNT          784 a         1333 c         1363 g          772 t
ORIGIN      
        1 ttcgtgccag gcagggggga cgcacctcgt cttcgcccgc aaccccggat agggtcgggc
       61 gtcctacgcc atgaagttga ccccggagat actcgaggcc tgttaccggg ccggtgcgtt
      121 cccgatggcc gactctcacg gacgcatcga gttctaccgc tccaacccgc ggagcgtgct
      181 cgagttggag gacctgcacg tctcccggtc gctggcgcgt gtgatccgca aaggcaacta
      241 cgaggtgcgc gtggacgagg acttcgaagg cgtcacacgc gcctgcgccg atagggagga
      301 gacctggatc aacgccgaga taatcgacgc ctacgtccgg cttcacagac aaggaaaagc
      361 ccacagcgtc gaagcctaca aagcaggcca gctcgttggg ggactctacg gcgtctctct
      421 aggtggggcg ttcatgggag agtcgatgtt cgcccacgcg agagacgcct ccaaagtctg
      481 cttcgtgcac ctagtggagc gtttgaggga gcgggatttc tcgcttctag attgccagat
      541 acagaactcc cacctcgcca gccttggggc gcgtgagatc cccgagcgcg agtacctgcg
      601 acgtctggga gaagccttga gcctggaccg ctcattcgtc tgacagcagc ttgcaatggg
      661 attcagcatt tcagccggtc agcacgtcag cacttcagca agaacaaggg ctgaaatgct
      721 gactagctga agtgctgaag tgcgcgaata gcagaggtct tgacctctgc tattcgcttt
      781 gaggatcggg ccttgcagcg tggtaagctt gttccatgga tatacgacac atcgacatcg
      841 acttcgaacc cgagctcgag cggccggtcc tcatcgccgg cttcactggc tggaacgacg
      901 ccgccgacgc ggcgagcgtc gccgtcggcg ccctggccag ctcctgggag gcgcggcgtt
      961 tcggcggctt cgacggcgaa gagttcttcg acttccagac cacgcggccc caggtcaaac
     1021 tggtagacgg cgttacgcga gagatcgaat ggcccgagaa cgtgctctca gcgacggagc
     1081 cggccctgga agccgcgggc ggacgcgggg gaatactgct gacgggacct gagccgaact
     1141 tccggtggcg cactttctct gcggcggtcg tcgagctggc taaagagttg gacgtggagc
     1201 tggtggtcac gatgggcgcc ctgctcgccg acgtgccaca ttcgcgtccg gtagcggtag
     1261 cggccaactc ccaggacgcg actcttgtcg aatccctggg cctctccgcc tcgcgctacg
     1321 agggaccgac aggcataacg ggcgtgttgc accgactctg cgccgacggt gggctgccct
     1381 ccgtgagctt ctgggcctcg gtcccgcact acctgcccgc ggttccgtct gcccccgccg
     1441 cgctcgcact cctcgagaag ctttcgagcc tactgggaat gcaggtggag acagccgacc
     1501 tacaaagcac ggccaagacc taccaggagc aggtctccgt ggcggtctcc caggactccg
     1561 acctctcctc ctacgtcagg atgctagaag agcgcttcga ctcgcaggcg gatcaaggcc
     1621 cacgcaatct gccatcgggc gacgaactcg cccaggagct cgaaggcttc ctccgcgagc
     1681 aacgcaatga tgaggaatag gttcaaggtt tctctggtcc agtgaacctt cgcctcgctg
     1741 gatagcttct gccctagatg gggcgtcacg agactcgact ctgcacttcc acgttggttg
     1801 ggcaaccact gctcgagcaa cgcccccttt ctggctggag gaacgggcct ggggctccac
     1861 gttctcgctt tcccagcgcc gcttgagcca acgcttgatc gaagtcagcg ataacccgaa
     1921 gtttcgcgcc acctcttgcg gggcatcccg cgatcgacgg cgtccaacac catgagccag
     1981 agatcctttg agtacgcttt catgccggta gtttccacaa ggatcaattt tcacgcaact
     2041 cgctgtaaca cgcgtttcgg gcgagagcag gtgcatcaga gttcatctga cgtctgaaaa
     2101 acctggcgtc tctaaagctc taatacacga gcgcaagcac gaagaccacg acgctcgcga
     2161 gtcctagcaa tccctggacg acggcggcgg ccaggtagcc gacgaagaca ccgccggccg
     2221 cccgtgaggc acggcgccag tcgctcccgc tggaggattg ttcctcggta ccctgccgtt
     2281 cgcgcttcag gtactcgccg aggaagacgc ctgcgatagt gccgaggatg aggccgaaga
     2341 tggagccgat accgaggaag aacaggccga tcaacgcgcc ggcgataccg cctattgctc
     2401 cgcctacggt accccagttg ctcgctccga agcggcgggc gccgtaggag gtggcgacga
     2461 agtcagcgac gaaggcgatc gccgcgaaca cgaagaggag cacgaggatc gcgacgccga
     2521 cggaacggaa gtccgtaatt atggcgtaga cgagggcgga gaggaagatg agcgtaacgc
     2581 cgggcagtcc cggcacgacg cttccaatca ggcccagggc catgacgatg agggtgagga
     2641 cgagaagcgt accctcaaca gagtccatcg ctacgacgag attactggta gcgtcttctt
     2701 cctgcccttc ttaagggcgg cattaaggct tccggacttg tatccggcca ggtcgagggt
     2761 gacgtagagg aaacctgcgt cgaggagctc ggtggagatc tcttcccgca tggcgaaggc
     2821 tctttcgagc tcatccggcc cgacttcgag gcgggctatc tccccgtggt gcctgacgcg
     2881 gacctgcctg aaaccctctc tgcgcaggaa ctcctcggcg cgcgcgacct gcgagagctt
     2941 ttggggtgtg atctcctgcc cgtaggggaa ccggctggag aggcacgcga gcgcgggctt
     3001 gtcccaggtc ggcagatcga ggtacttcgc catctcacgc acctcggcct tggtcatgcc
     3061 ggcctcggag agcacggaga cgaccccgag ctcccttgcg gctttcctgc cggggcggta
     3121 gtcgccctcg tcgtctttgt tcgccccgtc cacgacgcag gcgtagcctc tatcatcagc
     3181 caactgcgcc aggtccgagt agagagtgct cttgcagaag tagcagcggt tcgtcgggtt
     3241 gcttgcgtag ttcgggtcgt cgagctcgcg ggtgttcacg acgaggtgct ccaccccgag
     3301 tgacccggcg aactcgcgcg cgagatccaa ctccgaaggc agataggtct cgttgttgga
     3361 agtgacggcg agcaccctgt ctttggggag cgcccgcgca gccacggcga gggcgagcga
     3421 ggaatccacc ccgccggaga aggcgacgag cgcgcttccg tggggggcga ggatctcctc
     3481 tagctcctct agcctcttgt gcaaggtgtc ggtcgcgaca gggttcatgt gcacgtcctc
     3541 tctagcttgc tgtacgttcg gagagccagc ccaggacttc ttcggcgttc ctacctggcg
     3601 ggaatacggg atagaagacc tcccggatct ccccatcctc gatgaccagg gtcaggcgcc
     3661 ttataagtgt catgccttcg gcctcgaagg tcggaagatc tagtgatacc gcgaactcga
     3721 gcctctcatc gctcaacaac aggaacggga ggtgcagccg ttcggcggct tcgcgctggt
     3781 aggccgagtc ctgggtgctg aggccgaaga cgcgggcccc gagcgcctga agctccgcat
     3841 ggtggtcgcg gaaggcgcac gactgggggg tgcatccccg ggcgccaggg atatcgtccc
     3901 agccttgcgg caagtcctgg tccggcctcc ccgtcatcgg gtagcagtac acgacggtag
     3961 tacctgtcag agtcgaaaga tccacccggt ccccatctgt ggacgtcagg gataccgaat
     4021 gaagccgcat ccccggcagg tggccgcagg cgccgtcgtc cggcggttct gggatgccat
     4081 cgggcacgga gtacaggcta tcgttgcgcg tcatcaggcc ctctccagga ccacggccgc
     4141 gttgtgtcct ccgacgccga ggttggcgca gatggctgcc tcgaggtcgg gcgcctcgcg
     4201 cggctcgtcg acgacgtagt cgaggtcggc gcagccttcc gctactttcg tg
//
LOCUS       DATAIR010000373         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_24252, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000373 DATAIR010000000
VERSION     DATAIR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24252"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VHM69_09085"
     CDS             complement(<1..1043)
                     /locus_tag="VHM69_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2740588.1"
                     /translation="MTTFANVCAELDRRRHVTMGFERIEALLDMMDHPERDLPNVVQV
                     VGTNGKGTTAIALAAALEEMGHPSGAYLSPHVLSYTERVVLRGERVSEEEFAAVMGET
                     MQLADGHGIPVSQFEILTAGALRMFSGLSWAVLEAGLGARFDATSVAEPDAVVLTNVA
                     IDHTEYLGETIDEIAAEKLASLRPGATLFLGTDDERVVDIASSECSRVGATLVRVGEG
                     SREEIHSLAPYVASDVGLALAVAGSMLNLAIPAGVKEKVARDTGGALPGRFEVHEVDG
                     VPVVVDGGHNAAGVSAAVAAVRGAYGGLPLGVVFGVLREKDAASMLTGLGGDSHVIVL
                     TRPESERASDPAR"
     gene            complement(1063..>1922)
                     /locus_tag="VHM69_09090"
     CDS             complement(1063..>1922)
                     /locus_tag="VHM69_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2740589.1"
                     /translation="AYSYERAEMGRAFVTKLWNATRFVLAYPEAEDSGEPSPSDRWIL
                     SEFNRTVREYDALLEECEFSEAMRLIYSFAWNQFADWYIEIAKAVPSPATPRVLRQVL
                     HGTLALLHPVMPFATEEMAWVLGNEELLATQSFPEPDSEDEEADRLLDRTKRAVSAVR
                     SFRAESKVDGELEGLVPQGIDEGVFVALARVRPVDAPDGASRAALPAGDMVVEILLSE
                     EMRRGEIERLRREISRAEQEVRRAESKLANEKFVQNAPEKVVAGEREKLDANSRLLET
                     LSRRLDEYL"
BASE COUNT          320 a          694 c          579 g          329 t
ORIGIN      
        1 aagcgcgcgg ggtccgatgc cctctcgctc tcagggcgag tcagcactat cacgtgggag
       61 tcccccccca accctgtaag catacttgcg gcatccttct ctcgcaagac gccgaagacg
      121 accccgagcg gcagcccgcc gtaggcgccg cgcacggcag ccaccgccgc cgacaccccg
      181 gccgcgttgt gccccccgtc gaccaccact gggaccccat cgacctcgtg tacctcgaac
      241 cgtccgggga gcgccccccc tgtatcgcgc gcgaccttct ccttcacacc ggcagggatc
      301 gccaggttca acatggaccc ggccaccgcg agcgccaggc caacgtcgct cgccacgtat
      361 ggggcgaggg agtggatctc ctcccggctg ccttctccga ctcgaaccag cgtcgctccg
      421 acccggctgc actcgctact cgctatgtcg acgactcgtt catcgtccgt gccgagaaag
      481 agggtggcgc ccggtctcag gctggcgagc ttctcagccg ctatctcgtc gatcgtctcg
      541 cccaggtact cggtgtggtc gatggcgacg ttcgtgagca caacggcgtc cggttcggcg
      601 acggaggtgg cgtcgaaacg cgcgcccagg ccagcctcga ggacggccca cgacagcccc
      661 gagaacatcc tcagcgcgcc cgctgtcagg atctcgaact gcgagacggg gatgccgtgc
      721 ccgtccgcga gctgcatggt ctctcccata acggcggcga actcctcctc cgagacccgc
      781 tctccccgca ggacgacgcg ctcggtgtag gagaggacgt gcggtgagag gtacgcgccg
      841 gatggatgtc ccatctcttc gagggccgcg gcgagggcaa tggccgtagt ccccttgccg
      901 ttcgtgccga cgacctgcac gacatttggc aggtcgcgtt cgggatggtc catcatgtcg
      961 aggagggctt cgatcctctc gaaacccatt gtgacgtgcc tgcgccggtc caactcggcg
     1021 cagacgttcg cgaaggtggt caaggtgggt atattcgccc tcctagaggt attcgtccag
     1081 ccggcgagaa agcgtctcca gcaacctgga gttcgcgtcc agcttctcgc gctctccggc
     1141 gacgaccttc tcgggggcgt tctggacgaa cttctcgttt gcgagcttgc tctccgcgcg
     1201 cctcacttcc tgctcggcgc gcgagatctc ccgcctcagc cgctctatct cgccgcggcg
     1261 catctcttcg gagagcagta tctccacgac catgtccccc gcgggcaacg ccgcccggct
     1321 cgccccatca ggagcgtcca ccggcctcac ccgtgcaagc gccacgaaaa caccctcgtc
     1381 gataccctgc ggtacgagcc cttccagctc gccatcgacc ttcgactcag cccggaacga
     1441 tcgcaccgcg gagacggccc gcttcgtacg gtcgagcagc cgatcggcct cctcgtcttc
     1501 cgaatcaggc tcggggaagc tctgcgtcgc gagcaactcc tcattcccca ggacccacgc
     1561 catctcttcc gtcgcgaacg gcatgacggg gtgcaggagc gcaagcgtgc catgtagcac
     1621 ctgccgcagg accctgggcg tcgcgggcga gggtaccgcc ttcgctatct cgatatacca
     1681 gtcggcgaac tggttccagg cgaaggagta gatcagacgc atcgcctcgg agaactcgca
     1741 ctcctccagg agagcgtcgt actccctgac ggtacggttg aactcagaga ggatccaacg
     1801 gtccgaaggt gatggttccc ccgaatcctc agcctcaggg taggcgagga cgaagcgcgt
     1861 ggcgttccac agcttggtca cgaaggcccg gcccatctcg gcccgctcgt acgagtaggc
     1921 ga
//
LOCUS       DATAIR010000374        11191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_301406, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000374 DATAIR010000000
VERSION     DATAIR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301406"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1365)
                     /locus_tag="VHM69_09095"
     CDS             complement(121..1365)
                     /locus_tag="VHM69_09095"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2740590.1"
                     /translation="MSDTGSTLTHLEGALSGERYPADELAGLDPVDGRPLLARYDIER
                     AALTLTAESLARRPGGGLWRWKELLPVRDGRHVIYLGEGDTPLLAQPRLGEALGVPYL
                     ATKAEGLNPTGSFKARGMAAAVSRAVELGVTSFIAPSAGNAAGALAAYGAAAGARVTV
                     LTPVDATPVNQQEVLAMGARLILVRGLISDCGKISAALAGRIGAFDVSTLKEPYRVEG
                     KKTMGFEIAEQRGWRLPDVIVYPTGGGTGLVGMWKAFEELEALGLIGPERPRMVSVQS
                     EGCAPVVRAFEAGERFAQPWEDARTRASGIRVPSAIGDFLILDALRESGGTAVAVSED
                     AIDEMQMFAGRTGAGYVSPESAAALAAVPVLRDRGELDQDDEVVVFDCGVGQKYPPPE
                     LPKAPVVDPDDLDLDALERILG"
     gene            1563..2138
                     /locus_tag="VHM69_09100"
     CDS             1563..2138
                     /locus_tag="VHM69_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2740591.1"
                     /translation="MSNPIDNATNHDPPVTAVASRRVRPGREAEFEEWVSGILSAAAE
                     FPGYLGSNIIRPGDSDDDEFHIVFKFDHASNLKRWEGSHERREWLRKSRDLILEKENV
                     RVLTGLETWFTLPSRPGEPAPPRFKMAVVTWLAVFPLATAIFALTHPLLGGLPTVIRT
                     LVFTIIMVTLMTYVVMPRMTRLFSFWLYPKK"
     gene            complement(2263..3159)
                     /locus_tag="VHM69_09105"
     CDS             complement(2263..3159)
                     /locus_tag="VHM69_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2740592.1"
                     /translation="MNVEIVDVGPRDGLQNEDKTLPPEVRAELCEKLAAAGVPRVEAA
                     SFVNPKRVPQMAGAEDVMTSIERSPGTRYAGLVLNERGYERAIEAGVDEVRYAFPVTE
                     TFAKRNQNTTVADATHLAARLVERARLDGVRVSITLSAAFGDPFEGSVEPGRVLEIAG
                     TVAQSIPDEIVLADTIGVGVPSQVRTLVDGVAGNGITVGCHFHDTRNTGIANAAAAVE
                     SGATVLDASVGGTGGCPFAPRATGNIATEDLVYLLHGMGYETGIDLDALIEVADWLDR
                     QLGKELPGRVYKAGNFELGFRL"
     gene            complement(3159..4367)
                     /locus_tag="VHM69_09110"
     CDS             complement(3159..4367)
                     /locus_tag="VHM69_09110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2740593.1"
                     /translation="MGEEYGGALEGVRVVEMGSLLAGPFCGQLLADFGAEVIKLEPPG
                     KGDPMRVWGRHSKEGRTLWWPIIARNKKSVTLNLREEEGQDLARRLISGADVVVENFR
                     PGTLERWGLGYEKLSRINPGLVMVRVSGYGQTGPYKDQAGFGSIGEAMGGIRHVTGFA
                     DRPPPRVGVSLGDSLAATFGALGAVTALYNREARGGKGQVVDVGIYEAVLALMESTIP
                     EYALAGHIRGRTGAILPFVAPSNTYPTREGEYIVIGANADTVFGRFARAIGHPEWAES
                     ERYATHHARGENQEELDGLISGWTSQRTADEVLEVMREASVPAGKLFTAEDMLSDEQY
                     AARQNVVEVEDPEIGPFPMQNVVPRLSETPGGIRWTGPKLGQHNDEVFKDLLGMSEED
                     LTSLRERGVI"
     gene            complement(4360..5049)
                     /locus_tag="VHM69_09115"
     CDS             complement(4360..5049)
                     /locus_tag="VHM69_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2740594.1"
                     /translation="MGHVLRDGGKQSGSIGVLGRMRALILTGEYGPEERLIEEQLAER
                     LGVSRTPVRQALTMLEAEGLVEITPNRGATVCSFSIEDVWDIYDLRAVLEGHAARRAA
                     GRIERHELERLRELAVEMEGLAGRFEDHEEEIRALVDLNQEFHGTIAVASRNRRLKRL
                     IDRTVQIPLIFKAFFWYTPRERAISNHYHRRMIEALEQGDADRAEIIMREHVYEGRDS
                     VIGALEKDMNG"
     gene            complement(5161..7107)
                     /locus_tag="VHM69_09120"
     CDS             complement(5161..7107)
                     /locus_tag="VHM69_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2740595.1"
                     /translation="MSFDSVLVANRGEIAVRIIRVCRELGIGSIAVYSEADERAMHRR
                     LADKAYLLGPAPAPESYLNVERLVEAIERSGAEAVHPGYGFLSESAAFARAVESAGAV
                     WVGPPPEAMETVGLKVRAKDLARSAGVPTIPGYDGEDDGEDSLAEEADRIGYPVLVKA
                     SAGGGGRGMRAVTRPEDFVEAVRGARREAEAAFGDGSVFLEKLVEDPRHVEVQVIGDS
                     KGNVLHLFERECSIQRRHQKVVEEAPSPALSPDLRASICDAAVRFTREAGYRNAGTVE
                     FLLSGEEFYFLEMNARLQVEHPVTELVTGLDLVHLQLAVAAGEPLPLTQDDVTLRGSA
                     IEARLYAEDEQGMPSGGRLEVFSPPEGAGVRNDAGFEAGDEVPLNYDAMLAKLIVYAQ
                     DRETAVRRLRRSLADYEVLGPSTNLPLLRRIAAHQAFEAGETTIRFLEEHHLTEPPPA
                     RPIPRNALLLAAAAELNSIPASPDPFAAGPWRLLGSATLEYLPEHRVEAGRAGQGKLR
                     MILDGEESTIEVLYSGEGRIRALIDDEPVEAAVVLDREVVLVSLGSETHALRKPAPPS
                     VDEAGEGADAAASLTAPMPGTVVKVLVEEGEEVEGGQLLLVIEAMKMEQPVSAPHAGV
                     VRSLPFAEGALVPGGAVLAEVQES"
     gene            complement(7104..7850)
                     /locus_tag="VHM69_09125"
     CDS             complement(7104..7850)
                     /locus_tag="VHM69_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2740596.1"
                     /translation="MTVTLARPDSHNALNEALIEELTRCFEEISDDEGVRVVVLAGEG
                     RSFCAGADIGYMRDMADFSYERNLEDARRLAMMFWTIDECPKPVVAKVQGAAMGGGAG
                     LVAVADVAVVDSETNFAFSEVRLGIAPATIAPFVVRKVGASHARSLFLTGERFGADRA
                     REIGLAHEVVASDELDATVEEKVGALLGGGPVAQATVKALLRRLETTEPMEAPGLTSR
                     VISELRTGEEGQEGLEAFLEKREPSWRTGR"
     gene            complement(8013..9620)
                     /locus_tag="VHM69_09130"
     CDS             complement(8013..9620)
                     /locus_tag="VHM69_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2740597.1"
                     /translation="MSKLRTRIDSKSEAFTKNAEAFSSLMDELRRRSAEARRGGGEKS
                     QKRHRERGKLPVRERIERLLDPGTAFLELSPLAAFGMYDGKVPAAGIVTGIGRVSGRE
                     VMVVANDATVKGGTYYPMTVKKHLRAQEVAEQNYLPCIYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFFNQAKMSAKGIPQIAAVMGSCTAGGAYVPAMSDEVVIVKGTGTIFLGGPPL
                     VKAATGEEVTAEELGGADVHTRRSGVADHFAEDDEHALAIVRSIVENLNLKGDVPWTV
                     DEPEEPLYDPVELYGVVPSDYRQGYDVREVIARTVDGSRLHEFKPLYGETLVCGFARI
                     MGHPVGILANNGILFSESALKGAHFIELCCSRGIPLVFLQNITGFMVGKEYEAGGIAK
                     DGAKMVMAVANANVPKFTIIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGQQA
                     ANVLLTVQEEGMRREGQEMTDEDREEFRAPILEKYEDEGNPYHSTARLWDDGVIDPAD
                     TRMVLALGLEAAANAPLEETTFGVFRM"
     gene            9735..10529
                     /locus_tag="VHM69_09135"
     CDS             9735..10529
                     /locus_tag="VHM69_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740598.1"
                     /translation="MVGMSLFTPKEREEIADRVCRLLGDDGRVEGVVVVGSLAGRSDR
                     WSDIDIEVIIADDEDLTAVAADWVGSLYERFPVVHHFETAFGETLVRGFLLENLLELD
                     LAFEQAGRFSIWGPARVAFDRSGRVTEAASAAQGSPRRSDRPNEAGFAWHDVLHACTA
                     VRRGRPWQGLWYLERIRNRALTLAQERCGYYAEYFDYVDDLPAEEIEPFEDALVTSLD
                     SESLLSAIEVATRAFLAELRHGEPALADRLEGPLIEFVRLGASEPH"
     gene            complement(10548..10880)
                     /locus_tag="VHM69_09140"
     CDS             complement(10548..10880)
                     /locus_tag="VHM69_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX2740599.1"
                     /translation="MSLYALNLFDLADNDLYRRYSRRSVAAVGKHGGEVVALGRLDGV
                     VETEPGIEPRTVMVLVEWPSREAFHAFLEDPDHQDLHPLREDGTRKYLWWTYERLEDL
                     RPLLVGTT"
     gene            complement(10882..>11191)
                     /locus_tag="VHM69_09145"
     CDS             complement(10882..>11191)
                     /locus_tag="VHM69_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740600.1"
                     /translation="AMEIELARNMVLKAAWLRDQGRPFGREASMAKVFASEAAKRAAD
                     QAVQIHGGEGFMETSAVARYWRQVKINEIGEGTSEINRQIIARSLLKESAEAVKLVAQ
                     "
BASE COUNT         1838 a         3825 c         3391 g         2137 t
ORIGIN      
        1 gcgcttgtga cgctggcacc gttcgtccag gcctcttacg aagcttacag ggcctacatg
       61 ggcgggggcc gccgctacct gtaggctgag gatgaagccg tacctggaca agggtcttcc
      121 ctagcccagg atacgttcca gcgcgtccag atccaggtcg tcggggtcta cgacgggggc
      181 tttggggagt tcaggcggcg gatacttctg tccgacaccg cagtcgaaga cgacgacctc
      241 gtcgtcctgg tcaagctctc ccctgtcgcg cagtacgggg acggcggcga gggctgcggc
      301 tgactccggg ctgacgtatc cggctcccgt gcgtcctgca aacatttgca tctcatcgat
      361 tgcgtcttca ctcacggcca ccgccgtgcc gcctgattct ctcaaagcgt ccagaataag
      421 gaagtctcca atggcggaag ggacacggat gccggatgct cgggtacgcg catcttccca
      481 gggctgggcg aagcgttccc ccgcctcgaa cgccctgacg acgggcgcgc agccctccga
      541 ctgtacgctc accatgcgag gtcgttcggg cccgatcagg ccaagcgcct caagctcttc
      601 gaatgccttc cacatcccca cgagacccgt gcctccgcct gtcgggtaga cgatcacgtc
      661 gggcaggcgc cagccgcgct gttccgcaat ctcgaagccc atcgtcttct tgccctcgac
      721 gcggtagggc tccttcaggg tactcacgtc gaaggcgccg atcctccctg cgagggcggc
      781 tgagatcttg ccgcagtcgg agatcaaacc tcgtacaagt atgagacgcg cccccatcgc
      841 cagcacttct tgctggttga ccggcgtcgc gtccacgggg gtgagcaccg tgacccgtgc
      901 gccggcggcc gcgccgtatg ccgcaagggc tccggcagcg ttccccgccg acggcgcgat
      961 gaacgaggtc acacccagtt ccacggcccg cgaaacggca gcggccatcc cccgcgcctt
     1021 gaacgagccg gtgggattca agccttccgc cttcgtggcg agataaggga ccccgagcgc
     1081 ctccccaagt cggggctgcg ccaggagcgg cgtatccccc tcgcccaggt agatgacgtg
     1141 cctcccgtcc ctgacaggca acagctcctt ccaacgccac aggccgcctc ccgggcgccg
     1201 cgccagagat tcggccgtca gggtaagggc ggctctctcg atgtcgtaac gggcgagcag
     1261 aggacgcccg tccaccgggt caaggcccgc caactcgtcc gcggggtacc tctcccccga
     1321 aagcgcgccc tccagatgtg tcagcgtaga acctgtatcg ctcaaatcca cctctctcca
     1381 gaccgcagga attctactag caagctcaca ttattgcccc agggcgcttc gtccctcgat
     1441 gttcatagtc gggtcgccgg agggactggt ctgggatctt tccccctccg gccagtggtc
     1501 ggcgcgcggc tcgtagtctt taataggtgc atacagaagc cagcagcccg gagcagacct
     1561 ctttgagcaa tcccattgac aacgccacta accacgaccc gcccgtgacg gcggtggcct
     1621 cacggcgcgt gaggccgggt cgcgaggcgg agttcgagga gtgggtatcg gggattctca
     1681 gtgcagcggc ggaattcccc ggctacctcg ggtcgaacat cataaggcca ggcgactccg
     1741 atgacgacga gttccacatc gttttcaagt tcgaccacgc gagcaacctc aagcgctggg
     1801 agggttcgca cgagcgccgc gagtggctac gaaagtcaag ggatctgatt ctggagaagg
     1861 agaacgtgcg ggtcctcacc ggactggaga cgtggttcac gctaccttcg cggccaggcg
     1921 agcccgcgcc gccgcgcttc aagatggcgg tcgtcacctg gctcgcggtc tttccgcttg
     1981 cgaccgcgat cttcgcactc acccatccgc tactcggcgg acttccaacc gtgatccgca
     2041 cgctcgtctt caccatcatc atggttacgc tgatgaccta cgtcgtcatg ccgcgcatga
     2101 cacggctgtt ctctttctgg ctctacccga agaagtagct ttatcaggca acaggtttca
     2161 ggcttcagga aataggcagc gcatacgttg agagattgtc gacatgatcc tagcgcgagc
     2221 acccggcggg acggcagccc tcagatacct gaagcctgtt gcctatagcc tgaagcctaa
     2281 ctcgaagttt cctgctttgt agactcgtcc aggaagctct ttaccgagtt gccggtcgag
     2341 ccagtccgcc acttcgatca gggcgtcgag gtctattccg gtctcgtatc ccatcccgtg
     2401 caggaggtat acgaggtctt ctgtggcgat gtttccagtg gctcgcggcg cgaaggggca
     2461 tccacccgtg ccgccaacgg aggcgtcgag aactgtcgcg ccgctctcga cggcggctgc
     2521 tgcgttggcg atgcctgtat tacgcgtgtc gtggaagtgg cagccgacgg tgatgccgtt
     2581 gccggcgacg ccgtctacca gcgtccgcac ctggctcggc accccgaccc cgatagtgtc
     2641 ggcgagcacg atctcatcgg ggatcgattg cgctacggtg ccggcgatct cgagtacgcg
     2701 accgggttcg acgcttccct cgaaagggtc tccgaaggcg gctgaaagcg tgatggagac
     2761 ccgcacccca tcgagcctcg cccgctcgac gagcctcgcg gcgagatgcg tcgcgtctgc
     2821 gacggtcgta ttctggttgc gcttcgcgaa cgtctccgtc acggggaagg cgtagcgtac
     2881 ttcgtccaca cctgcctcga ttgcccgctc gtagcctctc tcgttgagga cgagaccggc
     2941 gtacctcgtt cccggggaac gctcgatgga ggtcatcacg tcttccgcgc cggccatctg
     3001 cgggacgcgc ttcgggttga cgaagctcgc cgcctcgacg cgggggactc cggccgccgc
     3061 cagcttctcg cacagctcgg cgcggacctc aggggggagc gttttgtctt cgttctggag
     3121 gccgtcgcgg gggccgacgt cgactatctc gacattcatc agatgacccc ccgctcgcgc
     3181 aggctggtga gatcttcctc gctcatcccc agcaaatcct tgaacacctc gtcgttgtgt
     3241 tgcccaagct tcgggccggt ccaccggatt cctcccggcg tctccgagag gcgcgggacg
     3301 acgttctgca tcgggaacgg tccgatctcc gggtcttcga cctcgacgac gttctggcgg
     3361 gcggcgtact gctcgtccga aagcatgtcc tccgcggtga acagcttccc ggcagggacg
     3421 ctcgcctctc tcatgacctc gagcacctcg tccgcggttc gttggctcgt ccagccggag
     3481 atcaaaccgt cgagctcttc ctggttctcg ccgcgggcgt ggtgggttgc gtaccgctcg
     3541 ctctcggccc actccggatg cccgatggcc ctggcgaacc ttccgaagac ggtgtcggcg
     3601 ttggcgccga tcacgatgta ttccccttcg cgcgtggggt aggtattgga cggcgcaacg
     3661 aagggcagga tggcgccggt gcggccccgg atgtgccccg cgagcgcgta ctcagggatg
     3721 gtgctctcca tgagtgcgag gacggcctcg tagataccca cgtccaccac ctggcccttt
     3781 ccgccgcgcg cctcgcggtt gtagagggcc gtcaccgcgc ccaatgcccc gaatgtcgca
     3841 gcgagtgagt cgcccaaaga gactccaact cgcggcggcg gacggtcagc gaacccggta
     3901 acgtgccgta tcccgcccat ggcctcgccg atcgatccga accccgcctg gtctttgtat
     3961 ggtcccgtct gtccgtaccc cgaaacccgc accatgacca gaccggggtt gatccggctc
     4021 aacttctcgt accctagacc ccatctctcc aacgtgcctg gcctgaagtt ctcgacgacc
     4081 acgtcggccc cggagatgag gcggcgggcg aggtcctgcc cctcctcttc ccgcaggttg
     4141 agtgtgacgg acttcttgtt gcgggcgatg atgggccacc agagcgtgcg tccctccttg
     4201 ctgtggcggc cccagacgcg catggggtcc ccctttccag gcggctccag ctttatgacc
     4261 tcggcgccga agtccgcgag gagctggccg cagaaagggc ccgcgagcag gctgcccatc
     4321 tcgacgacgc gaacgccttc gagggccccg ccgtactcct cacccattca tgtccttttc
     4381 gagcgctccg atgacggagt ccctcccctc gtagacgtgc tctcgcatga tgatctccgc
     4441 cctgtccgca tcgccctgtt ctaatgcctc gatcatgcgt cgatgatagt ggttggagat
     4501 tgcccgctcg cgcggcgtgt accagaaaaa cgccttgaag atgagcggta tctgcacggt
     4561 ccggtcgatg agacgcttga ggcgcctgtt gcggctcgcc actgcgatgg tgccgtggaa
     4621 ctcctggttg agatcgacga gagcccggat ctcctcctcg tggtcttcga accggccggc
     4681 caacccctcc atctcgacag ctagctcccg caaccgctcc agctcatgcc gctcaatacg
     4741 tcccgccgcc cgcctcgcag cgtggccctc cagtacagcc cgcaagtcgt agatatccca
     4801 tacgtcctcg atggagaacg agcacaccgt cgccccgcgg ttgggtgtga tctccaccaa
     4861 tccctcagct tccagcatcg tgagcgcctg acgtacagga gtcctgctca ccccgagccg
     4921 ctccgcgagc tgctcctcga tcaatcgctc ctcaggcccg tactcgcccg tcaggatcaa
     4981 agcccgcatc ctaccgagaa ccccgatgga ccccgactgt tttcctccgt cccttaggac
     5041 gtgtcccact caaacccccg ttcgtgacca aaggagaggc ttttgtatac aatcttaggc
     5101 ggctacggat tttgtgtcaa gagtttcaaa tccgggcaat cgttgtggtc atgcggcgcg
     5161 ctagctctcc tgcacctcgg cgagcacggc gcctcctggg acgagggctc cctcggcgaa
     5221 ggggagtgag cggacgacgc ccgcgtgcgg ggcggagacc ggttgctcca ttttcatcgc
     5281 ctcgataacg aggaggagtt ggcccccctc gacctcctca ccctcttcga cgagcacctt
     5341 cacgacggta cctggcatcg gggcggtgag gctcgcggcg gcatccgcgc cctctccggc
     5401 ctcgtccact gatggaggag cgggcttgcg gagagcatgc gtctctgagc caagcgagac
     5461 aaggactact tctcggtcaa gcacgaccgc cgcttcgacg ggttcgtcgt cgatcagggc
     5521 gcggattcgg ccctctcccg agtacaggac ctcgatggtg gactcttcac cgtcgaggat
     5581 catacggagc tttccctgtc ctgcgcgccc tgcctcgacg cggtgctcgg ggaggtattc
     5641 gagggtggcc gaaccgagca ggcgccatgg gcccgcggcg aacgggtcag gggaagccgg
     5701 gatgctattg agttccgcgg ctgcggcgag gagcagggcg tttcgtggga ttggcctggc
     5761 gggcggaggc tcggtcaggt ggtgttcttc cagaaagcgg atcgtggtct cgcctgcctc
     5821 gaacgcctga tgggccgcga ttcgccggag gagcgggagg ttggtcgagg gaccgaggac
     5881 ctcgtagtct gcgagtgacc gcctgaggcg gcgtacggcg gtctcgcggt cctgggcgta
     5941 gacgatcagc ttcgcgagca tcgcgtcgta gttgagcggg acctcgtccc cggcctcgaa
     6001 tccggcgtcg tttcgtacac ctgccccctc gggcggcgag aagacttcga gacgtcctcc
     6061 cgacggcatc ccctgctcgt cctcggcgta gagacgcgct tcgatggcgc ttccgcgaag
     6121 ggtcacgtcg tcctgggtga gcgggagcgg ctctcccgcg gcgacggcga gctggaggtg
     6181 gacgaggtcg aggcccgtca ccagttccgt cacggggtgt tcgacctgga ggcgggcgtt
     6241 catctcaagg aagtagaact cctcgcctga gagcaggaac tccaccgtgc ctgcgttcct
     6301 gtagccggcc tcgcgggtaa atcgcacggc ggcatcgcag atcgacgcgc ggaggtccgg
     6361 gctcagtgcc ggggacgggg cctcctccac gaccttctgg tggcggcgtt ggatcgagca
     6421 ctcccgttcg aagagatgga ggacgttccc tttagagtcc ccgatcacct gcacctcaac
     6481 gtgccgaggg tcctcgacaa gcttctccag aaatacagac ccgtctccga acgctgcctc
     6541 agcctcgcgc ctggcccccc tgaccgcctc gacgaagtcc tcgggacgcg tcacagcccg
     6601 catccctcgt cccccacctc ctgcgctcgc cttcacaagt accggatagc cgatacggtc
     6661 agcctcttcg gcgagactgt cctcgccatc atcctctccg tcgtagccgg gaatagtcgg
     6721 cacgcctgcg ctgcgcgcca gatccttcgc ccgcaccttc aagccgaccg tctccatcgc
     6781 ctcgggcggc ggtccgaccc agacggcacc ggcggattca acggcccttg cgaaagccgc
     6841 gctctcggac aggaagccgt agcctggatg tacagcttcc gcccctgaac gttcgatcgc
     6901 ctcgaccagc cgctcgacgt tcaggtagct ctcaggcgcg ggagcagggc cgagaagata
     6961 cgccttatcg gcgaggcggc ggtgcatcgc gcgctcgtcg gcctccgagt agacggcgat
     7021 gctgcctatg ccgagctcac ggcagacacg tatgatgcgg accgctattt cgccacggtt
     7081 ggcgacgaga acgctgtcga agctcaccgc ccggttctcc agctcggctc gcgtttctcc
     7141 aggaaagctt ccagtccctc ctgtccctcc tcgccagtac gcagctcgga gatcactcgc
     7201 gacgtcaggc cgggcgcctc catcggttcg gtggtctcga gccgcctgag gagcgccttc
     7261 accgtcgcct gagcgaccgg ccccccaccc agcaaggctc ctaccttctc ctcgacagta
     7321 gcgtcaagtt catcgcttgc aaccacttcg tgggcgaggc cgatctcccg cgccctgtct
     7381 gcgccgaagc gttcccctgt caggaacagt gacctggcgt gggaagctcc gacctttcgt
     7441 accacgaagg gtgcgatcgt ggccggcgcg atgcctagcc gtacctcgga gaaggcgaag
     7501 ttcgtctccg agtccaccac ggccacgtca gccaccgcaa cgagaccggc cccgccgccc
     7561 atcgccgctc cctggacctt cgccaccacc ggcttcgggc actcgtcgat cgtccagaac
     7621 atcatcgcca ggcgcctggc atcctcaagg ttcctctcgt aggaaaagtc ggccatgtca
     7681 cgcatgtacc ctatatccgc ccccgcgcag aacgagcgtc cctcgcctgc tagcaccact
     7741 acccggaccc cttcgtcgtc cgagatctcc tcgaagcagc gcgtcaactc ctcgatgagc
     7801 gcttcgttca gggcgttgtg cgagtcaggc cgcgcgagcg tgacggtcac caccgctccg
     7861 tcgcctgtcc tcctgaggtg ctcgtatcgc ataggcatca ggcttcagcc gccaggttgt
     7921 ctgtcgacgg cgtcggtagg atcccggcgc ggccagggtc gatgccacct cggacgtata
     7981 tcatgcgcac tccttcctga tacctacaaa tgctacatcc tgaagacacc gaaggtcgtc
     8041 tcttcgagcg gggcgtttgc tgctgcttcg aggcccagcg ccagtaccat ccgcgtgtca
     8101 gccgggtcta tgacgccatc gtcccagagc ctcgcggtag agtgataagg atttccctca
     8161 tcctcgtact tttcgaggat cggggctcta aattcctccc tgtcttcgtc tgtcatctcc
     8221 tgtccttcgc gccgcatgcc ctcctcctga accgtcagca acacgttcgc cgcctgttgg
     8281 ccgcccataa cgctgatgcg ggcgttcggc cacatccaca ggaaacgcgg cgagtatgca
     8341 cggccgcaca tcccgtagtt gcctgccccg aacgagccgc cgacgattat cgtgaacttc
     8401 ggcacgttgg cgttcgctac cgccatgacc atctttgccc cgtccttcgc gatgccgccg
     8461 gcttcgtatt ccttgcctac catgaagccc gtgatgttct gaaggaagac gagtgggatg
     8521 ccgcgggagc agcacagctc tatgaagtgc gcgcccttga gtgcgctctc ggagaacagg
     8581 atgccgttgt tcgccaggat acctacaggg tgacccatga tcctggcgaa gccacagacc
     8641 agcgtctcgc cgtacagcgg cttgaactcg tgcagtcggc tgccgtcaac cgttcgggcg
     8701 atgacttccc tgacatcgta gccctgcctg tagtcactcg ggacgacgcc gtagagttct
     8761 actggatcgt agagcggctc ctccggctca tccacggtcc acggcacgtc tcccttcaag
     8821 ttcaggtttt ccacgatgga gcggactatg gcaagggcgt gctcgtcgtc ctccgcgaag
     8881 tggtcggcca cgcccgagcg gcgggtgtgc acgtcggcgc cgccgagctc ttccgcggtg
     8941 acctcttcgc ctgtcgccgc cttgacgagc ggaggtccgc caaggaagat cgtgccagtc
     9001 cccttgacta tgacgacctc gtcgctcatc gcgggcacgt aggccccacc ggccgtgcag
     9061 cttcccatta ccgccgcgat ctggggtatg cctttcgcgc tcatcttcgc ctggttgaag
     9121 aagatgcgcc cgaagtgctc gcggtcgggg aagacctcgt cctgtaaggg gaggaaggcc
     9181 ccgcctgaat ccaccagata gatacacggc aagtaattct gctccgctac ctcttgcgcc
     9241 cgcaggtgct ttttgaccgt catcgggtag taagtaccgc ccttgacggt cgcgtcattg
     9301 gcgacgacca tcacctcgcg ccccgagacg cgcccgatcc ctgtcactat tcccgccgcc
     9361 gggacctttc cgtcgtacat cccgaaggcc gcaagggggg agagttcgag aaaggccgtg
     9421 ccaggatcga ggaggcgttc gatcctctct cgcacgggca gcttgccgcg ctctctgtgc
     9481 cgcttctgcg acttctcacc tccaccacgc ctggcctcgg ctgaccgcct gcgcagctcg
     9541 tccatgaggg atgagaacgc ttcggcattc ttcgtgaaag cctcgctctt cgaatctatg
     9601 cgggtccgca gcttgctcat ccgtctccca ttgcctggcc cgtacttcgg agccatgtta
     9661 gctacagcgc gccgcgtgcg ccaccgtacg tgcagaggga gggcaccgcc acgacacacg
     9721 gagcgtcgaa taaaatggtg gggatgtccc tgttcacacc caaagagcga gaggaaatcg
     9781 ctgatcgggt gtgccggctt cttggcgacg acggccgcgt cgagggcgta gtcgtcgtag
     9841 gctccctcgc gggccggagc gaccgctggt ccgacatcga catcgaggtg atcattgccg
     9901 atgacgaaga cttgacggcg gtcgccgcgg actgggtggg gagcctctac gagcgcttcc
     9961 ctgtcgtgca ccatttcgag acggctttcg gagagacgct cgtacgtggc ttcctgctcg
    10021 agaacctgtt ggagttggat cttgctttcg agcaggccgg acggttctcc atctggggtc
    10081 cggcgcgggt agccttcgat cgctcgggcc gcgtaacgga ggctgcttcg gcggcccagg
    10141 gaagtccccg ccggtcagat cggcctaacg aagcagggtt tgcctggcac gatgtcctgc
    10201 acgcctgcac cgccgtgcgc cgcggacggc cctggcaagg gctatggtac ctggagcgta
    10261 tccgaaaccg cgccctgacg cttgcccaag aacgttgcgg ctactatgcc gagtacttcg
    10321 actacgtgga cgacctgccg gccgaagaga tcgaaccgtt cgaagatgca ctcgttacat
    10381 ccctggattc cgagtccctg ctcagtgcga tcgaagtcgc cacacgggct ttccttgccg
    10441 agcttcgcca cggcgagcca gccctcgccg accggctcga aggtccgttg atcgagttcg
    10501 tccggctcgg cgcatccgaa ccgcactaac gacgcgcgga ggctccttca cgtcgtacca
    10561 actaagagcg ggcgcaggtc ttcaagccgc tcgtaggtcc accagaggta tttgcgcgtc
    10621 ccgtcctccc gcagcgggtg caggtcctgg tgatcgggat cttccaggaa agcatggaac
    10681 gcctcgcgag aaggccactc gacaaggacc attactgtgc gcggctcgat gccgggttcc
    10741 gtctccacaa caccgtcaag ccgcccgaga gccacgacct ctccaccgtg cttgccaacc
    10801 gcggcgacgg agcgccgcga gtaccggcga tagaggtcgt tgtccgccag gtcgaagagg
    10861 ttcagggcgt agagggacat cctactgcgc gaccagcttc accgcttcgg cactctcttt
    10921 caggagggag cgggcgatga tctggcggtt gatctcgctc gtcccctccc ctatttcgtt
    10981 gatcttgacc tgccgccagt agcgcgcgac cgcgctcgtc tccatgaagc cctcgccgcc
    11041 gtggatctgt accgcctggt cggcggcacg cttggccgcc tccgaagcga atactttcgc
    11101 catgctcgcc tcacgcccga aaggccgccc ctgatctctg agccacgcgg ccttgaggac
    11161 catgttgcgc gcaagctcta tctccatcgc c
//
LOCUS       DATAIR010000375         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_393417, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000375 DATAIR010000000
VERSION     DATAIR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393417"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VHM69_09150"
     CDS             <1..460
                     /locus_tag="VHM69_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome BD quinol oxidase subunit I"
                     /protein_id="HEX2740601.1"
                     /translation="METLRKLAVGALGLTALFTVLNLIPANANLIPQIGLVFGQGEDT
                     QIPLGSMYPYKYIGLIGMMLVGFFVIALYLRATATGFYWGRASRWSQFALMACAVTVV
                     LTMVTMGYTRETARRVDNDPGYLINECVTLQQEIVGEHCPTKPLSEGGSE"
     gene            457..606
                     /locus_tag="VHM69_09155"
     CDS             457..606
                     /locus_tag="VHM69_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740602.1"
                     /translation="MTDLALLLQEGSTGDYSAVGIFRFFLGFFLMITIYSSFLGFTYW
                     IGKDE"
     gene            complement(741..1463)
                     /locus_tag="VHM69_09160"
     CDS             complement(741..1463)
                     /locus_tag="VHM69_09160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2740603.1"
                     /translation="MTRRTAVVTGASSGIGEATARLFAQSGYNVVVAARREDRLKSIA
                     EDLGEGTLAVPTDVTDQAACEGLISRAVESFGSVDILVANAGLGLYGSIPGGEPEDWR
                     LMFEVNVLGVLYTTRAAVRHMLDRGQGDVVIVSSLAGRRVPGAEGTVYAATKHALTAV
                     AEGLRMDVHDKGIRVINVEPGLVRTQFPESSYPSAQEYYAQREFSPIEAEDVARAVLY
                     AVEQPRRVSVNEIVVRPTEQPK"
     gene            complement(1460..2251)
                     /locus_tag="VHM69_09165"
     CDS             complement(1460..2251)
                     /locus_tag="VHM69_09165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2740604.1"
                     /translation="MMEGGIHAGRVALVTGAGRGIGAATARLLAREGAAVALAARTED
                     EIVSVAREISSHGGTALAVSMDVADEDSVSSCFEKMRAELGNVTILVNNAGTPGVPLP
                     VAAMQPEEWRRVFDVNVTGAFHCAREALPGMASENWGRIVNVSSAAARHPVAGMAAYG
                     ASKAALDQLTRVLALEGAPYGITATGVYPGVIDTRMQEESRSFDQELIGKQLHRMFHG
                     YRDFGMLRPPEEPAELISYLCTPAARRLSGHIIRLEQLEALKGES"
BASE COUNT          395 a          762 c          779 g          468 t
ORIGIN      
        1 gatggagacg ttgcgcaagc tcgccgtggg cgccctgggc ctcaccgccc tgttcacggt
       61 gctaaacctg atccccgcca acgcgaacct gataccccag atcgggctcg tcttcgggca
      121 gggagaggat acgcagatac cccttggcag catgtaccct tataagtaca tcggcctgat
      181 cgggatgatg ctcgtcggct tcttcgttat agccctctac ctgcgcgcca cggcgacggg
      241 cttttactgg ggaagggcga gccggtggag ccagttcgcg ttgatggcct gcgccgtcac
      301 ggtggtgttg accatggtga cgatgggcta cacccgcgag accgcgcgcc gcgtcgacaa
      361 cgatcctggc tacctgatca acgagtgcgt caccctccag caggagatcg tgggcgagca
      421 ttgcccgacc aagccgctga gtgaaggagg gtctgaatga ccgacctggc gctgctcctg
      481 caggaaggaa gcacaggcga ctattctgcg gtcgggatct tccgtttctt cttagggttc
      541 ttcctcatga tcacgatcta ctcctctttc cttgggttca cgtactggat cggtaaggac
      601 gagtagagta ggcttcaggt ttcaggttgg ccgcgacgca agtcgcggcc ttctcttttg
      661 gtgcgtcact ttggaaaggc tccgtgcgcg aattcaggca gcgcctcaag acctgaagcc
      721 tactgcctgt agcctgaaac ctacttcggc tgctcggttg ggcggaccac gatctcgttg
      781 acgctcacgc gccggggctg ttcgacggcg tagagcacgg cccgggcgac gtcttctgcc
      841 tcgatgggtg agaactccct ctgcgcgtag tattcctgtg cgctcgggta tgagctctcc
      901 gggaactgcg tgcgaacgag gcctggctcg acgttgatga ccctgatgcc cttgtcatgc
      961 acgtccatcc tgagcccctc ggcgacggct gtcagggcat gcttggtcgc cgcgtagact
     1021 gtcccttcgg cccctgggac gcgcctgccg gcgaggctgg agacgatgac cacgtcgccc
     1081 tggccccggt cgagcatgtg acggacggcc gcgcgggttg tgtagagcac cccgagcacg
     1141 ttgacctcga acatcaggcg ccagtcttcc ggctccccgc cggggatgga gccgtagagc
     1201 ccgagcccgg cgttggccac gaggatgtcc accgagccga agctctctac cgcacgggag
     1261 atgagacctt cgcaggcggc ctggtcagtc acgtctgtcg ggacggcgag ggtaccttcg
     1321 ccgagatcct ccgctatgct cttcagcctg tcttcgcgcc gggcggcgac gacgacgttg
     1381 tacccgctct gtgcgaaaag gcgcgccgtg gcctcaccga tgccgctcga ggcccctgtc
     1441 acgacggctg tcctacgggt caagactctc ccttcagcgc ttcgagctgt tccaggcgga
     1501 tgatgtggcc gctcaggcga cgtgcggcgg gtgtgcagag gtaacttatg agttccgccg
     1561 gctcctcagg agggcggagc atcccgaagt ccctgtagcc gtggaacatg cggtggagtt
     1621 gtttgccgat gagttcctgg tcgaagctcc gcgactcctc ttgcatcctg gtgtctatca
     1681 cacctgggta gacacccgtt gcggtgatgc cgtacggcgc gccttcaagg gccaggacgc
     1741 gggtgagctg gtcgagggcg gccttcgaag cgccgtaggc cgccatcccg gcgacaggat
     1801 gcctggctgc ggcgctggag acgttcacta tgcgtcccca gttttctgac gccatgcccg
     1861 gcagggcctc cctggcgcag tggaaggccc ctgtcacgtt tacgtcgaag acgcgtcgcc
     1921 actcttcagg ctgcatggct gcgacaggga gggggacgcc cggagtaccg gcgttgttca
     1981 cgaggatagt gacgttgccg agttcggcgc gcatcttctc gaagcacgat gagaccgagt
     2041 cttcgtcggc cacgtccatc gacacggcga gggccgtgcc gccatgggag gagatctccc
     2101 gcgccacgga gactatctca tcttccgtgc gggctgcgag ggcgaccgcg gcgccctcgc
     2161 gggcgaggag gcgggctgtc gccgccccga taccgcgccc tgcgccggtt accagcgcca
     2221 cgcggccggc gtgtatcccg ccttccatca tctctggtag taaggagaga cgtatgtgcc
     2281 cgacgcaaca gatcccatct tccacccccg agctagacag ttcgtttcat ctagcttacc
     2341 ccacccgaag gccagcggca ggagatggaa acgcgcagta caggcgcgag gtgagataga
     2401 atgc
//
LOCUS       DATAIR010000376         7671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_439451, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000376 DATAIR010000000
VERSION     DATAIR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439451"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHM69_09170"
     CDS             <1..843
                     /locus_tag="VHM69_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX2740605.1"
                     /translation="GEGLGNQFLGHIRECEAIAHVVRCFEDDNVVHVHAGVDPEGDIE
                     TIDTELLLADLATVERRLERVGRAAKSGDARLKAEAAELEKLMGHLSDGCPARAYPES
                     ETLAEAMSTLISAKPTLYVANVDEEAVAEGNGYSSKVEKHAAREGAEAVRISAKLEAE
                     VAELSEDEAVEYLAMLGVETTGFEEFVRAAYRLLGLVTFFTAAEKESRAWTVHGGSTV
                     RRAAGRIHTDMERGFIAAEVGDWEDIVTAGSWAKAKEEAKIRLEGRDYVVKDGDTILV
                     RFNV"
     gene            847..1239
                     /locus_tag="VHM69_09175"
     CDS             847..1239
                     /locus_tag="VHM69_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX2740606.1"
                     /translation="MHFVRMKKPLDRSRLALVTTGGVHLSDQARFDIDDPAGDCSYRE
                     IPGEAKDLTWTHAYYRPDEGSDLDAVFPLITLSRLVEEGVVGELAPRHFSFMGAIHDP
                     TPLVESTAPEVAGKLVEDRVDAVLLTPS"
     gene            1315..1473
                     /locus_tag="VHM69_09180"
     CDS             1315..1473
                     /locus_tag="VHM69_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740607.1"
                     /translation="MEHGVSAPRVGFVPFPYNLPMGEPGDREGHRRIALAALALLHEL
                     EEPGEVEL"
     gene            1616..2311
                     /locus_tag="VHM69_09185"
     CDS             1616..2311
                     /locus_tag="VHM69_09185"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEX2740608.1"
                     /translation="MALLLVLFVAFFVVLFLFLLPILFSLQAVGSLFVYPRQLRAIFG
                     NKILRRNHALEHATIVVMMEREPGRKLNGFSTDDGYFVQGVRSIAEVESAAREAMRRL
                     QSGEKRLAIHRNCGTTIVAANMLAAVFFLVALGLGFLYLGGSYLYLLILGSVLLAIAL
                     RVPLSLLLQRFVTTDADLTNAEVGWVEPAKLGDLRSGIFGLLLAASTVQVRVFHTDPD
                     AVEIFRDDGAIVR"
     gene            2347..3723
                     /locus_tag="VHM69_09190"
     CDS             2347..3723
                     /locus_tag="VHM69_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX2740609.1"
                     /translation="MQNPGAYDVVIVGGGQAGVPLAHKLAAEGMSVALAERKDLGGSC
                     VNFGCTPTKSAIASAKVAHQARRASEYGLEIPEVRVDFPAVLRRAKEISVRRREGMES
                     GFEGSENPTLMRGHARLEGREGERFRLSVGEQSVLARHVVFNTGTRTQLPPIEGLDAV
                     AYMEAGNWLEHTELPEHLAVVGGSYIGLEMAQFYNRMGSRVTVVVGRSDHVAPREDDD
                     VTEALRTILEEDGIEFVLCERAKSVAAGEEGITLTLDGADRADMNISHLFLATGRLPN
                     TDDLGLENVGLKTIKGIIQVDERLATDVEGIWAAGDIRGGPMFTHTSYDDYRVLLSQI
                     AGDGSRTTTGRIVPYAIFTDPELGRVGMTEYEAREAGCEVEVMVFDMQRQSGKSREIG
                     ESKGLIKVVADASNGRILGAAVLTAEGSELVHMYVDLMNAGAPLDVLRDAIHIHPTLA
                     EDIQSAAF"
     gene            3952..4542
                     /locus_tag="VHM69_09195"
     CDS             3952..4542
                     /locus_tag="VHM69_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740610.1"
                     /translation="MAEALLPFGKKGPKPLKETGATRRRTSDGQTVTTFWEYPYFISS
                     NQDGVTVPEVRAWLRWVFQHSKSWVRAGVWFPEVRRSRDAMVVVRYVPGPIECGDVPD
                     AVGCTDRQMGPDKQTLIRMSSKRLATPQNPQGRFKGILHELGHAAFWATHGAGVVYSQ
                     RKAGLMAPGSPDGIWPTDVDIASVEAFTRGKNRVKG"
     gene            complement(4574..5131)
                     /locus_tag="VHM69_09200"
     CDS             complement(4574..5131)
                     /locus_tag="VHM69_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2740611.1"
                     /translation="MQRALEYASRLHTDQYRKGTRVPYITHLLAVAAIVGENGGTENE
                     VVAALLHDAPEDQGGLATLEDIRTRFGDEVAEIVEGCTDTYDDPKPPWRERKEAYVSH
                     LVGASDSVRLVSAADKLHNARTVLSDYRLLGDDLWERFNGGREGTLWYYRAIVDGLRK
                     VDSTPLIEDLDRVVTELERLAGAHA"
     gene            complement(5165..5992)
                     /locus_tag="VHM69_09205"
     CDS             complement(5165..5992)
                     /locus_tag="VHM69_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX2740612.1"
                     /translation="MRIYGLDFTSAPGRRKPLIALGCVLGGGTLRVEGSETLTDFGGF
                     EDFLQRDGPWVCGMDFPFGQPRSLVAALGWPESWEGYVGEVARLSKEEFEGAIRADMA
                     TRPPGSKWRYRLADRRSGSSSAMMLFRVPVGKMFYQGAPRLLASGIRVEPCRRNGDNR
                     VAVEAYPAVVARRFLGRTAYKRDGVPDTQERRTARRTLVAGLESAAFREVYGFSVEMD
                     GFWKEQFAGDPSADALDSLLCAVQATWAYLKRDENYGVPPECDPEEGWILDPALLTE"
     gene            complement(6011..7009)
                     /locus_tag="VHM69_09210"
     CDS             complement(6011..7009)
                     /locus_tag="VHM69_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2740613.1"
                     /translation="MKIPGMQWIKKAESKGNEAEVPMAENLGLVDFFKLTYKEVEEDH
                     VMAFAGNLTYKGLFAIFPFFTFLLSLLGIFNATDLVNTMIDKLSGVAPQSATTFIKGQ
                     LLSITQSQAESAFTVGAIISILLALWGVSGAFRSVMEAMNVMYAVEEDRPFWKVYGLS
                     IFMSLAVVVLLLGALVLVVFGESIGVTVADLVGLGSVFATIWSIVQWPIVACVVLLAF
                     ALIYYFAPAAEQKFRWISPGSLIAFVFWLIFSLLFSLYASTAGSYNETYGSLAGVIIL
                     MLYVYYSALIMLVGAEMNQVIEWHIPGGKNEGEKVPEEDRKPDVRRLRREEDREGR"
     gene            complement(7120..>7671)
                     /locus_tag="VHM69_09215"
     CDS             complement(7120..>7671)
                     /locus_tag="VHM69_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740614.1"
                     /translation="LFACSQAGLINNLNDGMSWGVFPLFFNTFGLGVERIGILKAVYP
                     AVWGILQVATGPLSDRWGRKGLIVAGMWVQAGGIFATVLGRSFGWWLLGSVLLGLGTA
                     MVYPSLIAGVSDASRPSWRARSLSVYRFWRDLGYAVGALSAGVISDALGLSWAIGSAG
                     ILTFLSGVVAAISMREGGNGDSK"
BASE COUNT         1467 a         2354 c         2482 g         1368 t
ORIGIN      
        1 ggggaggggc taggcaacca gttcctcggg cacatcagag agtgcgaagc catagcgcac
       61 gtcgttcgct gcttcgagga cgataacgtg gtgcacgtac atgctggagt cgaccccgag
      121 ggcgacatcg agacaatcga cacggagttg ctgctggccg atctcgcgac cgtcgagcgg
      181 cggctggagc gggtcggacg ggccgccaag agcggcgacg ccaggctcaa ggccgaggcg
      241 gctgagctcg agaagctcat gggccatctc tccgatgggt gtccggcgcg ggcttacccc
      301 gagtccgaga cgcttgccga ggcgatgagc accctgatct cggcgaagcc gacgctctac
      361 gtggcgaacg tggacgagga ggccgtggct gaagggaacg gctacagctc caaggtcgag
      421 aagcacgctg cgcgcgaggg tgctgaggcg gtcagaatat ccgcgaagct cgaagcggag
      481 gtcgccgagc tctccgagga cgaggccgtc gagtacctgg cgatgctcgg ggtagagacg
      541 acgggatttg aggagttcgt gcgcgccgcg taccggctgc tcgggctcgt caccttcttc
      601 accgcggcgg agaaggagag cagggcatgg accgtccacg ggggctcgac ggtgcggcgg
      661 gccgcggggc gcatccacac ggacatggaa cgcggcttta tcgcggccga ggtcggagat
      721 tgggaagaca tcgtgaccgc cggctcatgg gcgaaggcca aagaggaggc gaagatccgc
      781 ctcgaaggcc gtgactacgt cgtgaaagac ggcgatacga tactcgtccg cttcaacgtg
      841 taattgatgc atttcgtcag gatgaagaaa cctctagata gatcccgcct cgcgctcgtc
      901 actacgggag gcgtccatct gtcggatcag gcgcgcttcg acatagacga tcccgccggc
      961 gattgttcct accgggagat cccaggagaa gctaaggatc tcacgtggac ccacgcctac
     1021 taccgtcccg acgaaggctc cgacctcgac gccgtcttcc ccctcatcac cctcagccgg
     1081 ctggtcgagg agggcgtcgt gggagagttg gccccgcgcc acttctcatt catgggcgca
     1141 atccacgacc cgactccgct ggtcgagagt acggcccccg aggtcgctgg caaactcgtg
     1201 gaggacaggg tggacgcggt cctgctcact ccctcatgac ccctgtgcca ccggtccgtc
     1261 gggctggtct gcacagcaat agaacaaaga ggcatcccta ccgtcacgct ggcaatggag
     1321 cacggcgtca gtgcgccgcg cgtcggcttc gtgccgttcc cgtataacct gccgatgggc
     1381 gaacctggag accgtgaagg gcacaggcgg atcgcgctcg ccgcgctggc actactccac
     1441 gaactggagg agcctggcga ggtggaattg taggttacgg gcatcaggct acaggcatca
     1501 ggactttgat tgccggagat tgcctctggc acacaattgc ctgaaacctg aagcctgaaa
     1561 cctgaagcct gagatataat ctccgtacat agtgacctag tttgaaggtg agaccgtggc
     1621 cctgttgctg gtattgttcg tggcgttctt cgtggtattg ttcctcttcc tgctgcctat
     1681 cctgttctcg cttcaggctg tcgggagtct gttcgtatat ccgcggcagc taagggcgat
     1741 cttcggaaac aagatcctgc gccgcaacca tgccctggag cacgccacga tagtggtgat
     1801 gatggagagg gagcctggcc gcaagctgaa cgggttctcg accgacgatg ggtacttcgt
     1861 gcagggagtc cgctctatag ccgaggtaga gagcgcggcg agggaggcca tgcgcaggct
     1921 gcaaagcggg gagaagcggc tcgccatcca ccgcaactgc ggcacgacca tcgtggctgc
     1981 caacatgctc gccgccgtct tcttcctcgt cgcactcggg ctcggttttc tctatctcgg
     2041 cgggagctat ctctacctcc tcatccttgg cagcgtccta ctcgccatcg ccctgcgggt
     2101 ccctctgagc ctccttctgc agaggttcgt gaccacggac gccgacctca ctaacgctga
     2161 ggtcggttgg gtcgagccgg cgaagctcgg ggacctcagg agcggcatct tcgggctgct
     2221 tctagcggcc tctaccgtgc aggtgcgcgt cttccatacc gaccctgatg cggtcgagat
     2281 cttccgcgac gacggcgcga tagtccgtta gagatccgaa ataccagctc gtcgggaaag
     2341 ggaaacatgc agaaccctgg tgcttatgat gtcgtgatcg tgggcggcgg acaggcaggc
     2401 gtccctttgg cccacaagct cgcggcggaa ggcatgtccg tcgcgctcgc cgagcgcaag
     2461 gacctcgggg gctcgtgcgt caacttcggg tgtacgccaa ccaagtccgc catagcctcc
     2521 gcgaaggtcg cccaccaggc ccgccgcgcc tcggagtacg gcctcgagat tcccgaagtc
     2581 cgcgtcgatt tccctgctgt cctgcgcagg gcgaaggaga tctcggtgcg cagacgcgag
     2641 gggatggaga gtgggttcga aggttcggag aaccctaccc tgatgcgggg gcacgcccgc
     2701 ctggagggcc gcgaaggtga acgcttcagg ctaagcgtcg gcgagcagtc cgtgctcgcg
     2761 aggcacgtcg tcttcaacac gggcacgcgc acgcagctcc ctccaataga gggcctcgat
     2821 gcggtggcct acatggaggc ggggaactgg cttgagcaca ccgagcttcc cgagcacctt
     2881 gctgtggtcg ggggaagtta tatagggctc gagatggcgc agttctacaa ccggatgggg
     2941 agccgcgtca cggtcgtcgt ggggcgttct gaccacgttg ccccccgcga agacgacgac
     3001 gtgaccgaag ccctgcgtac gatcctggag gaggatggta tcgagttcgt cctgtgcgag
     3061 cgggcaaagt cggtcgctgc cggggaagag gggattaccc tcacgctcga cggcgcggac
     3121 cgtgcggata tgaatatctc gcacctcttc ctcgcgacgg gccgcctgcc caacaccgac
     3181 gatctcggac tcgagaacgt cggcctcaag accatcaagg ggataatcca ggtcgatgag
     3241 cgactggcga ctgatgtaga ggggatctgg gcagcggggg acatacgggg cggtccgatg
     3301 ttcacccaca cttcctacga cgactacagg gtacttctct ctcagatcgc gggcgacggg
     3361 tcgcgcacta ctacagggag gatcgtacct tacgccatct tcaccgatcc cgagctcggg
     3421 cgggtcggca tgaccgaata cgaggcaagg gaggccggtt gcgaggtcga ggtcatggtc
     3481 ttcgacatgc agcggcagag tggcaagtcg cgtgagatcg gggagagcaa gggcctcata
     3541 aaggtcgtcg cggacgcctc gaacggacgc atcctcgggg cggccgtgct caccgccgaa
     3601 gggtccgagc tcgtgcatat gtacgttgac ctcatgaacg cgggcgcgcc gctcgacgtg
     3661 ctcagggacg ccatccacat ccacccgacg ctcgccgagg acatccagag cgccgcgttc
     3721 tagctgcgta acaggcgtac ctccgctgtc cacgaggacc gcttccttac cgcaggctct
     3781 gtagcttctc tggagtactg cgcgcgaata tttctcggca gacaggcaac gttcattcag
     3841 aattcatcgc cgtgggctac aagggaacag cgtcggcatg gaaaggaggc ttggtgcgaa
     3901 tgaaaaggcg agttggagac aaatccagag cgagtcgaga ggagacagga catggctgaa
     3961 gcgcttcttc cctttggcaa gaagggacca aaaccgctta aagagacagg agctacgagg
     4021 cgaaggacct cggacggcca gaccgtcacg acattctggg agtatccgta ctttattagc
     4081 tccaaccagg acggcgtcac ggtgcccgag gtacgtgcct ggctccgctg ggtcttccag
     4141 cattcgaagt cgtgggtgcg ggcgggcgtg tggttcccgg aggtacggcg cagcagggac
     4201 gcaatggtag tggtccgcta cgtccctggc ccgatcgaat gcggagacgt tccagatgct
     4261 gtgggatgca ccgataggca gatggggccg gacaagcaga cgctcataag gatgagtagc
     4321 aagcgactcg caacgcccca gaacccccag ggacgtttca agggcatcct gcacgagctg
     4381 ggccacgccg cgttctgggc gacgcacgga gcaggggtcg tctactcgca gaggaaggcg
     4441 gggctcatgg ccccaggctc tccggatggt atctggccga cagacgtaga catcgccagt
     4501 gttgaggctt ttacgagggg caagaaccga gtaaaggggt agatagcgct tgcccgcatc
     4561 cagggctcac tggctaggcg tgagcgccgg cgaggcgctc caactcggtg actaccctgt
     4621 cgaggtcttc gatcaggggg gtgctgtcga ccttcctgag gccatcgact atggccctgt
     4681 agtaccagag cgtgccttcc ctaccaccgt tgaaccgctc ccagaggtca tcgccgagga
     4741 gcctgtagtc ggagaggacg gtgcgtgcgt tgtgtagctt gtccgccgcg gagacgagac
     4801 gtacggaatc cgaggcgccg accaggtggg acacgtaggc ctccttacgc tcgcgccagg
     4861 gcggcttcgg gtcgtcgtag gtatccgtgc agccctctac tatctccgcc acctcgtccc
     4921 cgaagcgcgt tctgatatct tcgagggtgg cgaggccacc ctggtcctcg ggtgcgtcgt
     4981 gcaggagcgc ggccactacc tcgttctccg tcccgccgtt ttcgccgacg atggccgcca
     5041 ccgccagcag atgggttatg tatggaacac gggtgccttt ccggtactgg tctgtgtgta
     5101 atcgagaggc gtactcgagc gctctctgca atttctctcc gtatgacatc tatcctcccg
     5161 gtctctactc tgtcaacagg gcggggtcga ggatccaacc ctcctcggga tcgcactcgg
     5221 gagggacccc gtaattctcg tcccgcttta gataggccca cgtcgcctgt accgcgcata
     5281 gcagcgagtc gagcgcgtcg gcggatgggt cgccggcgaa ctgttctttc cagaagccgt
     5341 ccatctccac gctgaagccg tagacctctc tgaaggctgc ggactcgaga ccggcgacca
     5401 gcgtccttcg cgcggtcctc ctctcctgcg tatcaggtac tccgtcccgc ttgtatgcag
     5461 tgcggcccag gaagcggcgg gcgacgaccg cggggtaggc ctcgacggcc acgcggttat
     5521 ccccgttgcg gcggcagggc tcgacgcgta tgcccgaggc cagaaggcgc ggcgccccct
     5581 ggtagaacat cttcccgacg gggaccctga agagcatcat cgcgctgctg gatccactcc
     5641 tgcggtcggc gagacggtag cgccacttgc tgcccggcgg tcgtgtggcc atgtcagccc
     5701 ggatcgcacc ctcgaactcc tccttcgaga gcctcgcgac ctcgcccacg tagccttccc
     5761 agctctcggg ccacccgagg gctgcaacca gggaccgtgg ctggccgaag gggaagtcca
     5821 tcccgcacac ccagggtccg tctctttgca ggaagtcctc gaagccaccg aagtcggtca
     5881 gcgtctccga gccctcgacg cgtagggtgc ctccccccag cacgcagcca agcgctatca
     5941 ggggtttgcg gcgacctggg gcgctcgtga agtcgaggcc gtagatcctc attcggagtc
     6001 gttcctggcg ttaccggccc tcgcggtcct cttcccgtct caaccggcgg acgtcaggtt
     6061 ttctgtcctc ctcggggacc ttctcaccct cgttcttgcc tcccgggatg tgccattcga
     6121 tcacctggtt catctccgcg cctacgagca tgatgagcgc agagtagtag acgtagagca
     6181 tcaggatgat cacaccggcg agcgacccgt aggtctcgtt gtatgagcct gcggtgcttg
     6241 cgtagagtga aaaaagcagc gagaagatga gccagaagac gaaggcgata agagagccgg
     6301 ggctgatcca gcggaacttc tgctcggcgg caggggcgaa gtagtagatg agggcgaagg
     6361 cgaggagtac gacacaggcc acgataggcc attgcacgat gctccagatc gtggcgaaga
     6421 cggaaccgag cccgacgagg tcggccacgg ttaccccgat ggactcgccg aagacgacca
     6481 gcacgagcgc cccgagcagg aggactacca cagcgagcga catgaagatc gagagcccgt
     6541 acaccttcca gaagggcctg tcctcctcaa ccgcgtacat gacgttcatc gcctccatga
     6601 cagaccggaa cgcccccgac actccccaga gggcaaggag gatcgagatg atcgccccga
     6661 cggtaaaggc gctctcggcc tggctctggg taatgctgag gagctggccc tttatgaacg
     6721 tcgtggccga ctggggcgcg acgccggaga gcttgtctat catggtgttg acgaggtccg
     6781 tggcgttgaa gatgccgagg agcgagagca ggaaggtaaa gaaagggaag atcgcgaaga
     6841 ggcccttgta cgtcagattg ccggcgaagg ccatgacgtg gtcttcttcc acctctttgt
     6901 acgtcagctt gaagaagtcg acgagcccca ggttctcagc catggggacc tctgcctcgt
     6961 ttcccttgct ctcagccttc ttgatccatt gcataccggg tatcttcatg cgcccgtcct
     7021 cctgtaggca tgtgtgccgc aatgctatac aaccagattt attccctgga cgcgatctat
     7081 ctaacaagcc cagccggagt cgatcccgca cgggtttgtc tacttgctat cgccattccc
     7141 tccttctcgc atcgagatcg cggcgaccac gccggagagg aaggtcagta tccctgccga
     7201 ccctatcgcc cacgacaggc cgagggcgtc ggagatcaca cctgcagaca acgccccgac
     7261 ggcgtagccg aggtcccgcc agaatcggta gacgctcagg gacctggccc gccaggaggg
     7321 gcgcgatgca tcggagacgc cggcgataag ggagggatag accattgccg tcccgagccc
     7381 aagcaacacg ctccctaata gccaccaccc gaagctcctg cccaacacgg tggcgaagat
     7441 cccgcccgcc tgcacccaca tgccagcgac gatgaggccc ttgcggcccc agcggtcgct
     7501 cagcggaccg gtcgcgacct gtagtattcc ccagaccgca gggtagaccg ctttgaggat
     7561 cccgatgcgc tccacgccga ggccgaaggt gttgaagaac agggggaaga cgccccaact
     7621 catgccgtcg ttgaggttgt tgatgagccc ggcttgcgag caggcgaaga g
//
LOCUS       DATAIR010000377         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_24379, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000377 DATAIR010000000
VERSION     DATAIR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24379"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHM69_09220"
     CDS             complement(<1..191)
                     /locus_tag="VHM69_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740615.1"
                     /translation="MGKREHYEPGTFCWVDLQTTDPAGAKAFYGGLFGWEAEDMPAGE
                     SGTYTMLSLDGDDVCAIYE"
     gene            334..945
                     /locus_tag="VHM69_09225"
     CDS             334..945
                     /locus_tag="VHM69_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX2740616.1"
                     /translation="MALYLLDLFGVAVFAVSGALTAGRKSMDLFGVIVVAVITAIGGG
                     TVRDILLDERQIFWIEDPMYLVVILAATVATILYGRFLTPPRISLLVADAFGLAVFTF
                     IGAQTAYAAGVPYLIVIVMGAITGSAGGIMRDVLCAEVPLILRRDIYATASVAGAIVF
                     VVLQEIGAGVPLTTLLPIATTFILRLAAIRFDLHVPPFRPKDG"
     gene            complement(972..1349)
                     /locus_tag="VHM69_09230"
     CDS             complement(972..1349)
                     /locus_tag="VHM69_09230"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2740617.1"
                     /translation="MAEKPEEVNESPTPEEKEDLKRRAQSDDEVEAAKANVELTRAEM
                     TDTVEALQEKLDPKSLKEEAKSTASDAGTRVVETAKQNPTVPVAVAGGLLGLLLVRRW
                     LRARGRRSDTVVIDLRRGRIRTF"
     gene            complement(1325..1714)
                     /locus_tag="VHM69_09235"
     CDS             complement(1325..1714)
                     /locus_tag="VHM69_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2740618.1"
                     /translation="MQEKDERSIGELFAELTRDTTALLRHEVQLAKAEMSQKASRVGK
                     NVGFLVVGGVVAYTGVLALVAAGIILLGQVIPYWLSAGIVGLVIALVGLFLVVKGANT
                     LRQEDPVPQETVETLKEDSQWLKNQKR"
     gene            complement(1802..2173)
                     /locus_tag="VHM69_09240"
     CDS             complement(1802..2173)
                     /locus_tag="VHM69_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX2740619.1"
                     /translation="MSPETNSPRITHLSWGRLETEGGVFKDARLFPGGAEEWDWNETG
                     TSHDPGIQPADVRRLLERGANVVILSRGFHERLGVAPETLRLLEVEGVPTHVAQTEEA
                     ARLYDELREKQKVGALIHSTC"
     gene            complement(2170..>2832)
                     /locus_tag="VHM69_09245"
     CDS             complement(2170..>2832)
                     /locus_tag="VHM69_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740620.1"
                     /translation="GFGGTKDSGGTPCRPDYAGSGGGTANPEFGGRLEAKDRTEGDPN
                     SPRNVMNNFYFKPPFRAAREEELLSSVLSTKTGEENYPGDMTLSENWPGIAPGEKGMN
                     NAISPKYCNLEDFALVQPGPPVLWVRGEDDAIVSDTSLLDFGYLGQLDAVPGWPGEEV
                     FPPQPMVSQIRAVLDAYEARGGRYHEEVFQDCGHTPHVEKPEEFRRLLFGFLDEQGGR
                     AG"
BASE COUNT          470 a          946 c          823 g          593 t
ORIGIN      
        1 atctcgtaga tggcgcagac atcgtcgccg tccaggctca gcatcgtgta ggttcccgac
       61 tctcccgcag gcatgtcctc agcctcccag ccgaagaggc cgccgtagaa ggctttcgca
      121 cccgccggat ccgtcgtctg aagatccacc caacagaacg tcccaggctc gtaatgctcc
      181 ctcttgccca tactctccct ttccgtcact ttggatcggc tgaaaagccg taccaaaatt
      241 ctacgagcgc gcgacacttt cccaagtagg agacgcgagc atcgagggaa ctacggcccg
      301 aaaacctcgg ccctggcttg ctaggctttg ggcatggctc tgtacctgct ggatctgttc
      361 ggggtcgcgg tgttcgcggt aagtggggcg cttactgcgg gccgcaagag catggacctc
      421 ttcggggtga tagtggtggc cgtgatcacg gctatcggcg gcgggacggt acgggatata
      481 ttgctggacg aacgccagat cttctggata gaggacccca tgtacctggt cgtgatcctg
      541 gcggctactg tcgctacgat cctctacgga cggttcctga cgccgccccg gatctccctt
      601 ctcgtcgccg acgccttcgg gctcgcggtc ttcaccttca tcggggccca aacggcctac
      661 gcggcggggg tgccttatct aatcgtgatc gtgatgggcg ccatcacggg ttcagcggga
      721 gggataatgc gtgacgtact ctgcgcggag gtgcccctga tcctacgccg cgacatctac
      781 gcgacggcgt cagtcgcagg cgccatcgtg ttcgtggttc tccaggagat cggggcaggc
      841 gtccccttga caaccctcct ccccatcgca acgaccttta ttctgcgcct cgccgccatc
      901 cggttcgacc tccacgtacc accgttccgt ccgaaagatg gatagaacta aaggccgtac
      961 tgttgcagtg gtcagaacgt cctgatcctg cccctcctca ggtcgatcac aaccgtgtcc
     1021 gacctgcgtc ctcttgctcg cagccaacgc ctcaccagca gcaacccgag cagacctccg
     1081 gcgaccgcga cggggacggt agggttctgc ttcgcggtct cgacgaccct ggtgcctgca
     1141 tcgctggccg tgctcttggc ctcctccttt agcgacttgg gatcgagctt ctcctggagg
     1201 gcttcgacag tgtctgtcat ctcggcgcgg gtaagctcga cgttggcttt tgccgcctct
     1261 acctcgtcat cgctctgggc cctgcgcttt aaatcttcct tctcctcagg cgtcgggctc
     1321 tcgtttacct cttctggttt ttcagccact ggctatcctc cttcagcgtc tcgaccgtct
     1381 cctgtggaac cgggtcctcc tggcggagcg tgttggcacc cttgacgacg aggaagaggc
     1441 cgacgagggc tatgaccagc ccgactatcc ccgccgagag ccagtacggt ataacctggc
     1501 cgagaaggat gatccccgcg gccaccagcg ccagcacgcc cgtgtaggca acgacgccac
     1561 ccaccacaag gaagcccacg ttcttgccga cccgcgatgc cttctggctc atctcggcct
     1621 tcgcgagctg gacctcatgg cgtaacagcg ccgtcgtgtc ccgcgtgagc tccgcgaaga
     1681 gctcgccgat cgacctttcg tccttctcct gcatcagact ctcctcgaga acgtcgtcca
     1741 ttctttcatg cccggttcgt tcccttttcg caccactcct aagcaacgac acagaggacg
     1801 gtcagcaggt cgagtggatg agagcgccta ccttctgttt ctcacgcaac tcgtcgtaga
     1861 gcctggccgc ctcttcagtc tgagcgacgt gcgtgggaac gccttctact tcaagcaatc
     1921 gcagggtctc tggcgcgacg cctagacgtt cgtggaaacc tctggagagg atcacgacgt
     1981 tcgcgccgcg ctccagcaat ctccgtacgt cggcgggctg tatgccaggg tcgtggctgg
     2041 tgccagtctc gttccagtcc cactcttccg cgccacctgg aaagagcctg gcgtccttga
     2101 agacgccgcc ttccgtctcc agacggcccc aggagagatg tgttatgcgt ggagaattcg
     2161 tctcagggct catcctgctc gacctccttg ctcgtcgagg aaaccgaaga gtaaccgcct
     2221 gaactcctcc ggcttctcga cgtgcggggt gtgcccgcag tcctggaaga cctcctcgtg
     2281 gtagcgtccg ccgcgggcct cgtaggcgtc gagcacggcg cgtatctggg agaccatcgg
     2341 ctggggaggg aagacctcct cgccaggcca tccggggacg gcgtcgagct ggccgaggta
     2401 gccgaaatcc agaagggagg tatccgagac gatcgcgtcg tcctcgccgc ggacccagag
     2461 caccggcgga ccaggctgta cgagcgcgaa gtcctccagg ttgcagtact tcggcgagat
     2521 ggcgttgttc atacccttct cacccggagc aatacccggc cagttctccg agagggtcat
     2581 gtcgccgggg tagttctcct cacccgtctt cgtggagagg accgaggaca gaagctcctc
     2641 ttccctcgcg gccctgaaag gcggcttgaa gtagaagttg ttcatgacgt tccttggaga
     2701 gttcgggtct ccctcggtcc tgtccttcgc ctcgaggcgc ccaccgaact cggggttggc
     2761 cgtcccgccg ccagagccgg catagtccgg cctgcatgga gtgccaccgc tatccttcgt
     2821 cccgccgaag cc
//
LOCUS       DATAIR010000378         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_393650, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000378 DATAIR010000000
VERSION     DATAIR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393650"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VHM69_09250"
     CDS             complement(<1..914)
                     /locus_tag="VHM69_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX2740621.1"
                     /translation="MIEASVLVVGAGAIGGVTAAKMKGRVRRVTVLDADREHVERMRD
                     PGLLLVDLGEEERVPLDAYGDPADLEGPFDFALVTLKAPHLEAALSPLVERGLAQTFV
                     SLGNGLVQDRIAGIVGNENLIVGTVEWGATNLGAGHLAQTTRAPFVIGELDGETSERT
                     RLLAEALETVADVRITENISGQVWSKLLVNSSFSGLGAVSGLLYREVVADPDGREAAL
                     AVWREGYDVGMAQGITLDQVLGVPAESLVVRSQVDRQRADEALETAMGYAGATKASML
                     QDLEKGAKTEVDVINGGVVEKGREYGVET"
     gene            complement(911..1900)
                     /locus_tag="VHM69_09255"
     CDS             complement(911..1900)
                     /locus_tag="VHM69_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740622.1"
                     /translation="MEDRSSHEGWPQATAVIGAGTMGLGVAECFAAAGMKVLLTDATP
                     ELTREAKRRLVQRARGHAGAGLLAEEAVGRVEAVETADDVAGTVGDVDFVFEAVPEDV
                     DLKREILGACSEAAPRDAIIVSNTSSLPINDLAEFVGRPELFCGMHWFNPPEWTPGVE
                     VIPAAATDTEVVGRLVEFLRGIGKRPAVVGDGPGFVANRIQNALFLEAVRCVEEGLAS
                     PQEVDEVVRSCFGFRLPFFGPFQISDMAGLDVYENVLDVLRDGLGERFEAPESLRKLV
                     EQGRTGTKSGAGFLQYTDEERERLLLERDRRYAALNELLKKLPPVNAGSEDGS"
BASE COUNT          320 a          722 c          587 g          365 t
ORIGIN      
        1 gtctcgacgc cgtattcgcg gcctttctcg acgacgccgc cgttgatgac gtcgacctcg
       61 gtctttgcgc ccttttcgag atcctgtagc atcgaagcct tcgtggcccc tgcgtagccc
      121 atcgctgttt cgagggcttc gtcagccctc tggcggtcta cctggctccg cacgactagc
      181 gactcggcgg gaacgccgag gacctggtcc aatgtgatcc cctgcgccat cccgacgtcg
      241 tagccctcgc gccagacggc gagcgccgcc tccctcccat ctggatcggc gacgacttct
      301 ctgtagagca ggcctgagac ggcgccgagg ccggagaaag agctgttgac gagcagcttc
      361 gaccacacct ggccgctgat gttctccgtg atacgaacat cggccaccgt ttcgagcgct
      421 tcggcgagca gacgggtccg ctcgctcgtc tcgccatcga gctccccgat cacaaacggc
      481 gcccgcgtcg tctgcgccag atggccagct cccaggttcg tcgcgcccca ctccaccgtc
      541 ccgacgataa ggttttcgtt tccgacgatt cctgctatgc ggtcctgcac gagcccgttg
      601 cccagagaga cgaacgtctg tgccagaccg cgttcgacga gaggactcag agcggcttcg
      661 aggtggggtg ccttcagggt tacgagggcg aagtcgaacg ggccttcgag gtcggcgggg
      721 tcgccatagg cgtcgagcgg cacgcgctcc tcctcgccaa gatcgacgag taggagaccg
      781 ggatccctca tcctctcgac gtgctcgcgg tcggcgtcga ggacggtgac acgccgtacc
      841 cggcccttca tcttcgcggc cgtcacgccg cctatggccc ctgccccgac tacgagtacg
      901 cttgcttcta tcacgaaccg tcctccgatc ccgcgttgac gggtggcaat ttctttagca
      961 gctcgttgag ggcagcgtag cgcctgtcgc gctcgaggag caacctctcc ctctcctcgt
     1021 ccgtgtactg gaggaaaccc gcgccgctct tggtgcccgt gcgtccctgc tcgaccagct
     1081 ttcgcagcga ctccggggct tcgaaacgct cgccgaggcc gtctcgcagt acgtcgagga
     1141 cgttctcgta cacgtccagt ccggccatgt cggagatctg gaacggaccg aagaagggca
     1201 ggcggaagcc gaaacagctc cgcaccacct cgtccacctc ctgcggcgaa gcaaggccct
     1261 cctccacgca ccggacggcc tccagaaaca gcgcgttctg gatgcggttc gccacgaagc
     1321 ccggcccgtc accgacgacg gcgggacgct tcccgatccc ccgcaggaac tcgacaagcc
     1381 gaccgacgac ctccgtatcg gtcgcggcgg caggtatgac ctcgacgccc ggcgtccact
     1441 ccggcgggtt gaaccagtgc ataccgcaga agagctccgg tcttccgacg aactcggcca
     1501 ggtcgttgat cggcagcgac gaggtgttcg agacgatgat ggcatcacgc ggcgcggcct
     1561 cggaacaggc gcccaggatc tcgcgtttca ggtccacatc ctcgggcaca gcctcgaaga
     1621 cgaagtccac gtctcctacg gtgccggcca catcgtcagc ggtctcgacg gcctccactc
     1681 ttccgacggc ctcctcagcc agcagaccgg caccggcgtg tccgcgtgcc cgctgcacca
     1741 gccgcctctt cgcctccctg gtcagctcgg gcgtcgcgtc cgtgagtagc accttcatac
     1801 cagcggcggc gaagcactct gcgacgccca gacccatcgt gccggccccg attacggctg
     1861 tggcctgcgg ccaaccttcg tgagagctcc gatcctccat ccgctaccag gtctcctcag
     1921 gtcggcagag atccagcgct tcccgtgtat ccatatggtt tttcgcccct ccaggttggc
     1981 tcgcttacca cttc
//
LOCUS       DATAIR010000379         7778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_150052, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000379 DATAIR010000000
VERSION     DATAIR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150052"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /gene="uvrB"
                     /locus_tag="VHM69_09260"
     CDS             <1..1833
                     /gene="uvrB"
                     /locus_tag="VHM69_09260"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX2740623.1"
                     /translation="EKAGSPALVIAHNKTLAAQLASEFADFFPNNAVEYFVSYYDYYQ
                     PEAYVPRTDTFIEKDAQINEDIDRLRHSATSSLFTRRDVIVVASVSAIYGLGSPEEYR
                     SKMVLLEQGGFYDLDDVLRDLVKIQYTRNDFQLNRGNFRVRGDVLEVHPAYQDTIYRV
                     SFFGDEVENMTEADPLTGEILREMDTLTVYPATHFVTGEDRLATAVKNIEAEMEERVA
                     ELEGDGKVLEAYRLKQRTQYDLEMMRELGYTSGIENYSRHLDGRPPGSTPYTLLDYFP
                     DDFVTFIDESHITLPQVRGMYNGDRSRKTTLVEFGFRLPSALDNRPLMWDEFLLKTDR
                     MVFVSATPGPYELENSGQIVEQIIRPTGLVDPEVEVRPTKNQIDDLLNEVAKRVEHGE
                     RVLITTLTIKMSEDLTDYLLEHNVKARYMHSNIETLDRIQIIRGLRTGEFDVLVGINL
                     LREGLDLPEVTLVAILDADKEGFLRGERALIQTIGRAARNVKGRVIMYADKETDSMRV
                     AIGETDRRREIQRSYNEQHGIEPRTITKGVSDILIAAEAKGLYKAGRKSGREAPRDPD
                     ELRTLIADLEAEMLEAAEELKFEYAAKLRDEIKDLERQLQEIAS"
     gene            complement(1842..2423)
                     /locus_tag="VHM69_09265"
     CDS             complement(1842..2423)
                     /locus_tag="VHM69_09265"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2740624.1"
                     /translation="MPRTKEFEPEAALHSAMELFWSKGYEATSMRQLLDGMGIGRGSF
                     YDTFGDKRTLFLATLDRFEQTRTAWVLEALEEGESPMEAIRYVFERTVDGLVGFEPRR
                     GCLLANTAVELAPHDPEVAERISRYIRRTEDAFERALQRARSAGEISEQQDPRSLARF
                     LVNSLHGLRVTARAGADRQTLEDTVEVVLGALR"
     gene            complement(2440..3696)
                     /locus_tag="VHM69_09270"
     CDS             complement(2440..3696)
                     /locus_tag="VHM69_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740625.1"
                     /translation="MTKSSLWKSPALVIVCGCLIALITYGVRTSFGLFTEPLSEGRDW
                     SREVFALSIAIQNLIWGVAQPAAGAFADRYGSARVLAAGGAVYAVGVGLMAFSGTPLS
                     MQLTGGVLVGLGLAGGSFTIVIAALGRLVSEERRSWAMGMATAAGSFGQFIFAPLGQS
                     FISTYGWQTALVFLAACTLLVPVLATALRSDKGSGGLTDEPAVPMRDAIRSAFGHGSY
                     LLLVAGFFVCGFHVAFITTHLPAHLSDAAAQSHAHGVHTSGAAQLGAWALAIIGLANI
                     VGSYTAGILGGKTSKRKLLSGIYLSRAAVIALFVTLPPTTVVVVAFAALMGVLWLSTV
                     PLTSGLVAVMFGTRHLGALFGFVFLSHQVGAFLGVWLGGLAYEATGSYDPVWWAGVGL
                     ALIAAALHWPIVERRAPLPTVPEPAR"
     gene            complement(3859..4674)
                     /locus_tag="VHM69_09275"
     CDS             complement(3859..4674)
                     /locus_tag="VHM69_09275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2740626.1"
                     /translation="MRRFASRHPYLFSGLITLILFGLTFASRAAFPSTPVGNISKLPQ
                     EAFEPPIGLDLIISDMKSPDTLFWALAVLLGLGLLAWAGWLREAGFNRPAQWRNLHLL
                     LFPLLVCALTLSSGIFVPGPASLASVFLTVLIATFGEEIIFRGLVWRALAPTGPVRAM
                     ILTSLLSGLLILGRTATDGPWPEAARITALTVCSGFTYGALRWRTTSIWPVILVHTTF
                     AFAIDIATLGTITYRLMMILSTIGFLTYGIYLLRNRQARADGGVLQPEPARVR"
     gene            complement(4766..5392)
                     /locus_tag="VHM69_09280"
     CDS             complement(4766..5392)
                     /locus_tag="VHM69_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX2740627.1"
                     /translation="MAVTKRRPLDRRWILETAVRFVDREGLEALSMRKLGAELGVEAM
                     SLYNHVPNKEALLDGMVEVILGELKVPPVGEDWEERVREAYRAFRRLAHEHPNVFPLL
                     VIRPPDTMDGVWLVEEFLKTLGEAGFQPETALHAFRALSSYATGYAMAEIRGFAMEPA
                     EAHLGAKSLSAAEFPHISELDDYLEDVDRDAEFEFGLDLILAGLRKKR"
     gene            complement(5523..5804)
                     /locus_tag="VHM69_09285"
     CDS             complement(5523..5804)
                     /locus_tag="VHM69_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740628.1"
                     /translation="MEAPITQATGHNNTIVGVYEDRIELKSGWQSQNVESVGLRDVAA
                     IGIKGLVNVTLTIETNTGRVYQLNRMALPDARRIKNTFEQQKRRAGLYD"
     gene            complement(5940..7355)
                     /locus_tag="VHM69_09290"
     CDS             complement(5940..7355)
                     /locus_tag="VHM69_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2740629.1"
                     /translation="MRRLALLSTTVLLLVLALPGLALAQKGPSTAELSLAMDTMWVII
                     AACLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAIAALLFWAVGFALAFGTGNAL
                     IGTEGWFLAVPAGQVNDVYAGLSWTQVPLSAKFLFQVAFCAVSLAIVWGTMLERTKFA
                     VYVIFAVVFAGLIYPMVGHWIWGGGWLTELGMQDFAGSTVVHLSGAMAALAGTLLLGP
                     RLGKYDDDGRPVTIPGHNMPLAVLGVLILWIGWWGFNPGSTMAAIPDIADIALTTNLA
                     AAAGVLGAMGMSYLYRRNVDVGMGGNGAIAALVAITAPCAFVAPWASIVIGFVAGVIM
                     YATLVYVDKIGVDDPLGAIAAHGMGGIWGTLSCGLFTTPALAAVGEPGLFYGGGLYQL
                     GIQALGIVAAGGFVFLASLAVFATLKATIGIRVKPEQELDGLDIHEHGVFGYPDLVAT
                     DYQNGNGKIPDPMEHVTESTT"
BASE COUNT         1562 a         2420 c         2489 g         1307 t
ORIGIN      
        1 gagaaggccg gtagcccggc gctcgtcatc gcgcacaaca agacgcttgc cgcgcagctg
       61 gccagcgagt tcgcggattt cttccccaac aacgcggtcg agtacttcgt cagctactac
      121 gactactacc agcccgaggc gtacgtcccg cgcaccgaca cattcatcga gaaggacgcc
      181 cagatcaacg aggacatcga ccgcctgagg cactccgcca cgagtagcct cttcaccagg
      241 cgcgacgtca tagtcgtcgc gagcgtgtcc gcgatctacg gtctcggtag tcccgaggag
      301 taccgttcga agatggtcct ccttgagcag ggcggtttct acgacctgga cgatgtattg
      361 agggacctcg tcaagatcca gtacaccagg aacgacttcc agctaaacag gggaaacttc
      421 agggtgaggg gcgacgtgct ggaggtccat cccgcttacc aggatacgat ctaccgggtc
      481 tccttcttcg gcgacgaggt cgagaacatg accgaggccg accccctgac cggtgagatc
      541 ctgcgcgaga tggacaccct gaccgtctac cccgcgacgc acttcgtcac gggcgaagac
      601 aggctcgcga ccgcagtcaa gaacatcgag gcggagatgg aggagcgcgt cgcggagctt
      661 gaaggcgatg gcaaggtcct cgaagcgtac aggctcaagc agaggacgca gtacgacctc
      721 gagatgatgc gcgagctcgg ctatacctcc ggcatcgaga actactcgcg ccacctcgac
      781 ggaaggccgc cgggctcgac gccgtacacg cttctggact acttccccga cgacttcgta
      841 acgttcatcg acgagtcgca cataacgttg ccccaggtca gaggcatgta caacggcgac
      901 aggagcagga agacgacgct cgtcgagttc ggtttcaggc tgcctagcgc tctggacaac
      961 cggcctctca tgtgggacga gttcctgctc aagacggacc ggatggtctt cgtctcggcg
     1021 acgccggggc cgtacgagct cgagaactcg gggcagatag tggagcagat catccgtccg
     1081 accggcctcg tcgacccgga ggtcgaggtg cggccgacga agaaccagat cgacgacctc
     1141 ctcaacgagg tcgcaaagcg cgtcgaacac ggcgagcggg tacttatcac gactcttacc
     1201 atcaagatgt ccgaggacct gacggactac ctcttggagc acaacgtcaa ggcgcgctac
     1261 atgcattcga acatagagac gctggaccgc atccagatca tccgtggcct gcgtaccggc
     1321 gagttcgacg tgctcgtagg catcaacctc ctgcgcgagg gcctcgacct cccagaggtc
     1381 acgctggtcg cgatcctcga tgcggacaag gaaggcttcc tgcgcgggga gcgggcgcta
     1441 atccagacca tcgggagggc ggccaggaac gtcaaagggc gggtcatcat gtacgccgac
     1501 aaagagaccg actccatgcg ggtcgcgata ggcgagacgg accgcagacg tgagatccag
     1561 cgctcataca acgagcagca cggcatcgag cctcgcacca tcaccaaagg tgtctctgac
     1621 atcctcatcg cggcggaggc gaaagggctc tacaaggcgg gaaggaagtc gggccgcgag
     1681 gcaccacgcg accccgacga gctccgcacc ctcatcgccg acctcgaagc cgagatgctt
     1741 gaggcagccg aggaactcaa gttcgagtac gccgccaagc tgagggatga gatcaaagat
     1801 ttagagaggc agctacagga gatcgcttca tagcgccggg actaccgtaa cgccccgaga
     1861 acgacctcga cagtatcttc cagggtttga cggtccgcgc ccgcccgcgc cgtgacgcgc
     1921 aggccgtgca ggctgttgac gaggaagcgg gcgagagatc tggggtcttg ctgttctgag
     1981 atttcacccg cggagcgggc gcgttgtaag gctcgctcga aggcgtcttc ggtgcgcctg
     2041 atgtagcgtg agatcctctc cgcgacctcg gggtcgtgcg gcgcgagctc tacggcggtg
     2101 ttggcgagaa ggcacccgcg tcttggctcg aaaccgacga ggccgtcaac ggtcctttcg
     2161 aagacgtacc tgatggcctc catcggcgac tcgccctctt ccagggcctc cagtacccac
     2221 gccgtcctgg tctgctcgaa ccggtcgagc gtggcgagga agagcgtgcg tttgtccccg
     2281 aaggtatcgt agaagctgcc gcggccgatg cccatgccat cgaggagttg gcgcatggag
     2341 gtcgcctcgt agcccttact ccagaatagc tccatcgcgc tgtgcagcgc ggcttctggc
     2401 tcgaattcct tggtgcgtgg catctcttgg gtccctccgt tacctcgctg gttcaggcac
     2461 cgtggggagc ggggcgcggc gctcgacaat gggccagtgc agggctgcgg cgatgagcgc
     2521 gaggccgacg ccggcccacc acacggggtc gtaggagccc gtcgcctcgt aggcaaggcc
     2581 gccgagccag accccgagga aggcgccgac ctggtggctg aggaagacga agccgaaaag
     2641 ggcgccaagg tggcgggtgc cgaacatgac cgcgacgagc ccagaggtga gcggcacggt
     2701 cgagagccac aatacgccca tcagggcggc gaaggcaacg accacgaccg tggttggcgg
     2761 cagggtgacg aagagcgcga tgacggcggc tctggagagg tagatccccg agagcaactt
     2821 gcgcttgctc gtcttcccgc cgaggatgcc cgccgtgtat gagccgacga tgttcgccaa
     2881 cccgatgatc gccagcgccc acgctcctag ctgggcggcg ccggaggtat gcaccccgtg
     2941 cgcgtgcgac tgggcggcag cgtccgagag gtgagcaggc aagtgagtgg ttatgaaggc
     3001 gacgtggaag ccgcagacga agaagcccgc gacgaggagc aggtagctgc cgtgcccgaa
     3061 agccgaccgt atcgcatctc tcatggggac cgcaggctcg tccgtgagcc ctccgctgcc
     3121 cttgtcgctc ctcagggccg tggcgagcac cggtacgagg agcgtgcagg ccgccaggaa
     3181 caccagcgcg gtctgccagc cgtaggtcga gatgaagctc tgccccaaag gcgcgaatat
     3241 gaactgcccg aacgagcccg ccgccgtcgc catccccata gcccacgaac gccgctcctc
     3301 ggagacgagc ctgccgaggg ccgctatcac gatggtgaac gacccgccag ccagccccag
     3361 cccgacaagc acgccgcccg tcaactgcat ggatagaggt gtgccgctga acgccatgag
     3421 cccgacgccg acggcgtaca ccgccccgcc ggcagcaagc accctcgccg agccatacct
     3481 gtccgcgaac gccccagcgg caggctgcgc cacgccccag atgaggttct gtatggctat
     3541 ggatagagcg aagacctccc tgctccagtc acgcccctcc gagagcggct cggtgaaaag
     3601 cccgaagctg gtcctgacgc cgtaggtgat taaagctata agacacccac atacgatcac
     3661 caacgccggc gacttccaga gagacgactt tgtcatcgac caacccgcct gatccccatg
     3721 tgtcccgccg cccctcgcac tacactcctc ctaaaaccgt cgctcgtaac gccatgaccg
     3781 ccatcataca gatattggaa cgattggtca aatatttagt ggactggtaa gttgagagga
     3841 gtttccgata tcctggctct acctcactcg ggcgggttct ggctggagaa caccaccgtc
     3901 ggcgcgcgct tggcggttgc gaaggaggta gataccatag gtaaggaatc cgatggtgct
     3961 gaggatcatc atcaggcggt acgtgatggt accgagagtc gcgatatcta tggcgaaggc
     4021 gaatgtggtg tggactaaga tgacgggcca gatggaggta gtgcgccagc ggagcgcgcc
     4081 gtaggtgaag ccgctacaga cggtcagtgc cgtgatgcgg gctgcttcgg gccatggtcc
     4141 gtctgttgcg gtcctgccga gaatgagcag gccggagagc agagaggtca ggatcatggc
     4201 ccgtacggga ccagtcggcg ccagcgcgcg ccacacgagg ccgcggaaga tgatctcctc
     4261 gccgaaggtg gcgatgagca cggtaaggaa caccgaggca agtgacgcgg gccctgggac
     4321 gaagatgccg ctcgagaggg tcagcgcgca gacaagcagc gggaacagga ggaggtgcag
     4381 gttccgccac tgggctggcc ggttgaagcc ggcctcccga agccagcctg cccaggcgag
     4441 aagaccgagg ccgagcagga ccgccagagc ccagaacaga gtatccgggc tcttcatatc
     4501 cgagatgatc aggtcgagtc cgatgggagg ctcgaaggct tcctgcggga gcttcgagat
     4561 gttgcccacg ggcgtactcg ggaaagcggc cctgctcgcg aaagtgagcc cgaataggat
     4621 cagtgtgatc aggccggaga agaggtaggg gtggcggctg gcgaaccgcc tcaccggcat
     4681 actcctctac acggtctcca ggagtcccgc ggagagtgag gcgtgaaaga tcttccagag
     4741 gagacgttca ctgtggcggg tgatatcagc gcttcttccg aaggccggcg aggatgaggt
     4801 cgagtccgaa ctcgaactcc gcgtcgcggt ccacatcctc cagatagtcg tccaactcgg
     4861 agatgtgggg aaactctgcc gcggagagtg atttggcacc gagatgggcc tccgcgggct
     4921 ccattgcgaa gccacggatc tccgccatcg cgtaccccgt ggcgtagcta gagagcgccc
     4981 ggaaggcgtg caacgcggtc tcaggctgga agcccgcctc cccgagcgtc ttcaggaact
     5041 cttcgacgag ccagactccg tccatcgtgt ccggcgggcg gatgaccagg agagggaaga
     5101 cgttgggatg ctcgtgcgcg agacgccgga acgcccggta agcttcccgg acccgctcct
     5161 cccaatcctc gccaaccggt ggaaccttaa gttcgccgag aatgacctca accatcccgt
     5221 cgagtagtgc ttccttgttt ggtacgtggt tgtagagcga catagcctcc acccccaatt
     5281 ccgctcccag tttccgcatg gagagcgcct ccagcccctc cctatccacg aaccgcaccg
     5341 ccgtctccag tatccatctc cggtcgaggg gccgcctctt cgttactgcc atccctgaac
     5401 ctacctcatt tcttgtcgga atcccgcatt gcgggtgctt acttgcagtg taagtctaca
     5461 ccgcaaatcg attcgtgtgg tggaaatcag agaaaccggg atcggggtga gggcagttgt
     5521 ctctaatcgt agaggccggc cctgcgcttc tgttgctcga atgtgttctt gatcctgcgc
     5581 gcgtctggga gggccatgcg gttgagttga tagacgcgcc ccgtgttggt ctctatcgtc
     5641 agggtgacgt tgaccaggcc cttgatgccg atcgccgcga cgtcacgcag tccgacgctc
     5701 tccacgttct gggattgcca gccgctcttg agctcgatgc ggtcctcgta gacgccgacg
     5761 atggtgttgt tgtgtcccgt tgcctgcgtt atgggtgctt ccaagctgtc ctccgtgacg
     5821 ctccctattt gccttcggca acaggataca agacggcgtt accgatgttc gcttccagaa
     5881 gcccggacgt gaaaaaacac cggccgggga gatccggccg gtgcgtgcgt gtggctgctc
     5941 taggtggtgc tttcggtgac gtgctccatg ggatccggga tcttcccgtt cccgttctgg
     6001 taatcggttg ccacgaggtc ggggtatccg aagacaccat gttcgtggat gtcgagaccg
     6061 tcgagctcct gttccggctt gaccctgatg ccgatagtcg ccttgagcgt cgcgaagaca
     6121 gcgagcgagg cgaggaagac gaagccgccg gctgcgacga tgccaagcgc ttgaataccg
     6181 agctgataga ggccgccccc gtagaagagg ccaggctcac cgactgcggc gagggccggc
     6241 gtggtgaaga ggccgcagga aagcgtgccc cagataccgc ccataccgtg cgccgcgatg
     6301 gcgccgagtg ggtcgtcgac cccgatcttg tcgacgtaga cgagggtggc gtacattatg
     6361 acgccggcga cgaagccgat aacgatcgag gcccacggcg cgacgaaggc gcagggcgcg
     6421 gtgatggcga cgagggccgc gatggcgccg ttgccgccca tcccgacgtc cacgttgcgc
     6481 ctgtacaggt agctcatccc catcgcgccg aggacgccgg ccgccgcggc caggttcgtc
     6541 gtgagggcga tgtctgcgat gtcaggaatg gcggccatcg tcgagcccgg gttgaagccc
     6601 caccagccga tccacagtat aaggacgccg aggaccgcga gcggcatgtt gtgtcccgga
     6661 attgtaaccg gccgtccgtc gtcatcgtac ttgccgagac ggggaccgag gagaagcgtg
     6721 ccggcgagcg cggccatcgc gccggagagg tgcacgaccg tcgagcccgc gaagtcctgc
     6781 atcccgagct cggtcaacca gccgccgccc cagatccagt gtcccaccat cgggtagatg
     6841 agcccggcga agacgacggc gaagatcacg tagacggcga acttggtccg ctcgagcatc
     6901 gtgccccaca cgatggccag agagacggcg cagaacgcga cctggaagag gaacttggca
     6961 gagagcggca cctgcgtcca cgagagcccg gcgtatacgt cgttgacctg ccctgcaggg
     7021 acggcgagga accagccctc cgtgccgatg agcgcgtttc cggtgccgaa cgcaagcgcg
     7081 aagccaacgg cccagaacag cagcgccgcg atcgccaggt tgaccaggat cttagcgacc
     7141 acgctcccca cattcttcat ccgcgagaag ccgacctcga gcatggcgaa gcctgcctgc
     7201 atgaacagga cgagacaggc cgcgatgatg acccacatgg tgtccatagc cagcgagagt
     7261 tccgccgtcg aaggaccttt ctgggcgagc gccagcccgg gtaacgcaag caccaacaac
     7321 agaaccgtgg tcgataatag tgcgagcctc cgcaaaagct tcctccttgg aagtcgggta
     7381 tatacgtatg gaacatagtc taggcaccag cgccgaaatg gtctttagcc gaatgtacaa
     7441 agatgggtcc ccgcctgata ttgtcgttct ggcgaatgta caagtggcgg ggacgccagc
     7501 tagtcagcac gctacgtcct tcggacttcg ctttcagcac gcttcgggcc tagcggccct
     7561 cgctttcagc ttgtcagctt ttcctcggac ggcgccgccc tcgaactgcg gtcactcact
     7621 ccacaccccc ttgctgacaa gctgaagtgc tatctatcag cgaaacttga tggtttttcg
     7681 gggacgcggc cgagaagttg catggccttt atccccagag ttaggcgttc tcggtcctcg
     7741 ctcttgtcca cgtcgaggcc cgtgagctcg ctcacaga
//
LOCUS       DATAIR010000380         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_301711, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000380 DATAIR010000000
VERSION     DATAIR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301711"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..468)
                     /locus_tag="VHM69_09295"
     CDS             complement(112..468)
                     /locus_tag="VHM69_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740630.1"
                     /translation="MPRTPYVLLFEEVVPGAQPLTILVDDRGAVPLFDSEGKAGDFLS
                     STDFGADLEAVEVSRAGLLRALESVEDEVGYVAVNPPPAGEGPMMVRMGGLRELIDAL
                     QQSQEDDLFGLGGISS"
     gene            complement(487..807)
                     /locus_tag="VHM69_09300"
     CDS             complement(487..807)
                     /locus_tag="VHM69_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740631.1"
                     /translation="MEIRSMADSVAFSEEKMKKNALFDSPHLFYDAYCLLPGQSQKVH
                     AHEGSDKVYYVLRGEGRFTVGEEERDLGEGSAVIARAGDPHGVSNETQENLVLLVTMA
                     PRPS"
     gene            complement(991..2154)
                     /locus_tag="VHM69_09305"
     CDS             complement(991..2154)
                     /locus_tag="VHM69_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2740632.1"
                     /translation="MNKDEGQNLLVIGAPERDADAYHLAGFLAPDAVICLRVAGKRYL
                     AVSSLEYGRAAKEAPVDELLSYEELGIFRLARELKSGAKAYAAAVANLLEKLGASNSP
                     VLVPAHFGVVFADELRARGVTLAPDGKLFESLRRAKTEEEISHIAKTQDAVEAACAHA
                     VEVLEEAEVADDGILEWRGETLTSELLRAEIDMELLRRGCAAEGTIAAGGPQGADPHE
                     RGHGPLKAGESIIVDIFPMDLSTRYHSDMTRTFVKGEPNEGLQKMYDAVLASQEAALS
                     MIRAGVNGRDVHRKVSDILHEAGYKTSVHDQEEGKPLTEGFFHGTGHGVGLEIHEAPR
                     VSLADEELIPGDVVSVEPGVYDPKVGGVRIEDLVVVTEDGCRNLTKFPKEFVL"
     gene            2247..2489
                     /locus_tag="VHM69_09310"
     CDS             2247..2489
                     /locus_tag="VHM69_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX2740633.1"
                     /translation="MQKQRDMQSRVEEALDKVRPALRADGGDARVVACDEKNGTVSIQ
                     MLGACKGCPLSQLDFVYAIESLIRREVPDVREIVAV"
     gene            complement(2630..3151)
                     /locus_tag="VHM69_09315"
     CDS             complement(2630..3151)
                     /locus_tag="VHM69_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740634.1"
                     /translation="MFKLYLTSIVAAEIVALAAIEFLAPGLLIFFLAPIVPLTLVVAL
                     VVRRRYGSLAHSSRWLSPEAIVRHRARGSATTSPEALSHAIVERAEEIQRALEESPAD
                     EVRVEMCALGYRACANDMITLTHLTNEALPYASVLKRVKLRRSRKRAIDALSEARKAL
                     PPGALRAAPQERQ"
     gene            3214..>3523
                     /locus_tag="VHM69_09320"
     CDS             3214..>3523
                     /locus_tag="VHM69_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740635.1"
                     /translation="MASAILAVCIIPLVHSDIAHEFDLQRPVASAVAVIRTVLFSPRS
                     FYLNFKVEGPLKEPVLFVLLVGAVAGVLGAAVALASNLLFGDIDAGDIRSAVVDALLF
                     A"
BASE COUNT          642 a         1114 c         1099 g          668 t
ORIGIN      
        1 attacttccg caacaacggg taccagccgt actgccaggt gatcatcgcg cccaaggtag
       61 ccaagttccg caaagagcac ctcgacaggc tcaaggcgta ggaaggccgc tctagctgga
      121 gattccgccc agcccgaaga ggtcgtcctc ctggctctgc tgtagtgcgt cgatgagctc
      181 ccttaggccg cccatcctta ccatcatggg accctcaccg gccggtggcg ggttgacggc
      241 gacgtacccg acttcgtcct cgacggactc gagcgccctg aggagcccag cccgcgagac
      301 ctccaccgcc tcgaggtctg caccgaaatc cgtggaactg aggaagtctc cggccttgcc
      361 ctccgagtcg aagagcggca ccgccccgcg gtcgtccacc aggattgtga gtggctgcgc
      421 gccagggacc acctcctcga agaggagtac gtagggagtg cgtggcatct cgtccgaggc
      481 cacgattcag gaaggtcgag gagccatcgt gaccaggagg accaggttct cctgcgtctc
      541 gttactgacg ccatgaggat ccccggcacg ggcgataacg gcgctgccct cgccgaggtc
      601 ccgctcctcc tcgcccacgg tgaagcgacc ctcgccgcgg aggacatagt acaccttgtc
      661 cgagccctcg tgggcgtgga ccttctggga ctggccgggg aggaggcagt aagcgtcgta
      721 gaaaaggtgc ggcgagtcga aaagcgcgtt cttcttcatc ttctcctccg agaacgcaac
      781 cgagtccgcc atagatctaa tctccaaaag ctctcctcct tcgacgttgt cgtgacaccc
      841 actctagcat cccaccgagg ataggcttca gccaatcttg agatagcata cggagacaac
      901 aacacttcct ctgcaactgt aagaactgcg aatgtcatgc gccgcaagcg ttagccgatc
      961 acgaggaagc tgacggctga aacctgaaac ctacaaaaca aactctttcg ggaacttcgt
     1021 caggttgcgg cagccgtctt ctgtgacgac gacgaggtct tcaatacgga caccgcccac
     1081 cttcgggtcg tagacgccag gctcgacgga gacgacgtcg ccggggatga gctcttcgtc
     1141 ggcgagggag acgcgtggag cctcgtggat ctccaaccct accccatgcc ccgtgccgtg
     1201 gaagaatccc tccgtgagcg gcttgccttc ctcctgatcg tggacgctcg tcttgtagcc
     1261 ggcttcgtgc aggatatcgg agactttacg gtggacgtcc ctgccgttga cgcctgccct
     1321 gatcatggag agtgcagcct cctggcttgc caggacggcg tcgtacatct tctgtaatcc
     1381 ctcgttcggc tcgcccttga cgaaggtgcg agtcatgtcg gagtggtagc gggtcgagag
     1441 gtccatcggg aatatgtcga cgatgatgga ctctccggcc ttcagtgggc cgtggccgcg
     1501 ctcgtgcggg tcggccccct gcggacctcc cgcggcgatg gtaccttcgg cggcgcagcc
     1561 gcggcggagt agctccatgt ctatctccgc gcgcagcagt tcgctcgtca gggtctcgcc
     1621 tcgccactcg aggataccgt cgtcggcaac ctcggcctcc tcgagtacct cgacagcgtg
     1681 ggcgcaggcg gcctcgacgg cgtcctgcgt cttcgcgatg tgggagatct cctcctcagt
     1741 cttcgcgcgt cgcaggctct cgaagagctt gccgtcgggc gcgagggtga cgccacgggc
     1801 acgcaactcg tcggcgaaga cgactccgaa gtgggccggt acgaggacgg gcgagttcga
     1861 cgcaccgagc ttttcgagga ggttcgccac cgcggcggcg tacgctttgg cgccgctctt
     1921 cagctcgcgg gcgagacgga agatcccaag ctcttcgtag gagagcagct cgtccacggg
     1981 cgcttccttc gcggccctcc cgtactccag agatgagacg gcaaggtacc tcttgcctgc
     2041 cactcgcagg cagatcaccg cgtcaggcgc gaggaacccg gcgagatggt aagcgtcggc
     2101 atcgcgctcc ggcgccccga tgaccagcag attctgccct tcgtccttgt tcataataat
     2161 ccttccacga aaacaggccc ggcgtcccat tctactaccg gtccaaacga aatccgtccc
     2221 cgtactcgga tacaatgagc cgcgagatgc agaaacaaag ggacatgcaa agccgggtag
     2281 aagaggcact ggacaaagtc cgacccgcgc tgcgcgccga cggtggagac gccagggtcg
     2341 tggcgtgcga cgagaagaac ggcaccgtca gcatccagat gctcggcgcc tgcaaaggat
     2401 gcccgctctc gcagctcgac ttcgtctacg ccatcgaaag cctcatccgg cgcgaggtgc
     2461 ccgatgtacg cgagatagtt gctgtttagg tttcaggcta caggtttcag gcatcagacg
     2521 ggtgtgagag cgtcttcgag aatttcggct cgtcctccac agccccaaaa acggggaaag
     2581 gcacgcgcag ccaacgttag ccgaccacag aagagctgat gcctgaagcc tactgcctct
     2641 cttgcggtgc ggcgcgcagg gcgcctgggg ggagagcctt cctggcttcg gagagggcgt
     2701 cgatcgcccg cttccggctt cggcggagct ttacgcgctt gaggacagag gcatagggga
     2761 gcgcttcgtt ggtgaggtgg gtcagggtga tcatatcgtt cgcgcaggcc ctgtatccaa
     2821 gggcgcacat ctcgacgcgg acctcgtcgg cgggcgactc ttcgagggcg cgttggatct
     2881 cctcggcccg ttctactatg gcgtgggaga gcgcctcggg ggaggtcgtg gccgaaccac
     2941 gggccctgtg gcgcacgatg gcctcgggtg agagccagcg gctgctgtga gccaggctgc
     3001 cgtacctgcg gcggacgacc agcgccacta cgagcgtgag cgggacaatg ggagcgagga
     3061 aaaagatcag cagaccgggc gcgaggaact cgatcgccgc gagagctact atctcggccg
     3121 ccactatcga ggttaggtag agcttgaaca ttctctgcgg tacctctccg ccaccatttt
     3181 cgagggtctt aagtctatgt tttgcggagc gatttggcaa gcgctatcct ggctgtctgt
     3241 ataatcccgc ttgtgcacag cgatattgct catgagttcg accttcaacg ccccgttgcc
     3301 agcgccgttg cggtgatacg gaccgtactc ttctccccga gaagcttcta cctgaacttc
     3361 aaggtcgaag ggcctttgaa ggagcccgtg ctcttcgttt tgctcgtcgg cgcggtcgcc
     3421 ggcgtcctcg gcgcggcggt cgccctcgct tccaaccttc tcttcggtga catcgacgcc
     3481 ggggacatcc ggtcggcggt cgtggacgct cttctcttcg cgg
//
LOCUS       DATAIR010000381         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_242186, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000381 DATAIR010000000
VERSION     DATAIR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242186"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VHM69_09325"
     CDS             complement(<1..796)
                     /locus_tag="VHM69_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2740636.1"
                     /translation="MSYHVNGKTFSNEPAPGQCLRTFLRELGWFGVKKGCDTGDCGAC
                     TVWLDGVTVNSCIIPAFRAEGRRVTTIEGLAKDGSLHPMQRSFIESQGFQCGFCTAGM
                     IMVSSNLNEEERQDLPRYLKGNLCRCTGYRAIEDAIQGRKSIDDDQPGAACGANLPAP
                     ASVGIVTGKVRYTLDTEIEDLLHLKVLRSPHAHARIVAIRKEAALATPGVHEVFTWED
                     VPRRLYTTAIHDDHLVDPDDTYMLDNVVRFVGQRVAAVVADSEAAAE"
     gene            complement(807..1634)
                     /locus_tag="VHM69_09330"
     CDS             complement(807..1634)
                     /locus_tag="VHM69_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX2740637.1"
                     /translation="MNLNTITDVRRVTGEGDEDFEWREGDSWLAGGTWLFSEPQPHLR
                     RLLDLHGLNWEPLVVGEQGLRISPICTIAQVERLEAPPEWTAAPLIQQCCHAFLASFK
                     VWNTATVGGNICMSLPAGPMISLTVALEGVYTLRLRDGSERRVAADEFVTGDHQNILQ
                     PGDLLRSIELPIAALRKRTCFRRISLTHLGRSTALLIGTLSPQDGTFMLTVSASTVRP
                     VRLSFDAVPDARSLRERLRETIADSMYHDDVHGAPDYRKHMTYHFAEEIRGDLSQAA"
     gene            complement(2709..3392)
                     /locus_tag="VHM69_09335"
     CDS             complement(2709..3392)
                     /locus_tag="VHM69_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740638.1"
                     /translation="MNRRQTNNLVRLGILALPLSGLLTLWGLLGRYTAPNPRVDFEAA
                     AKVASSTSFIVSQFVGNIGGLALLILGIFALTAYLANSRARSLAVAAMILSILSLALV
                     LPALGVTTYTLPVLGHAYLNGQEDAKAIGDALFGNPLRNAIYIPVFALYATGFILFGV
                     AIWRSGILRKGAALALGIHAPLVSSFIKPQPNLLVVLGALLFIMGGVFITLDVFRGPP
                     TSRRREGPE"
     gene            complement(3429..>3936)
                     /locus_tag="VHM69_09340"
     CDS             complement(3429..>3936)
                     /locus_tag="VHM69_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2740639.1"
                     /translation="NLARWIPERDRIYRQIMRQGWNEQRRAFVQHYDTDVLDASVLLM
                     PLVKFITPTDPRWLSTLDAISDELVSDSLVYRYNPAASPDGLAGDEGTFSICSFWYVE
                     ALARAGRLDEARLTFEKMLSYANHLGLYSEEIGPTGELLGNFPQAFTHLALISAAFNL
                     DRALGRTT"
BASE COUNT          796 a         1173 c         1182 g          785 t
ORIGIN      
        1 cctccgcggc tgcctcgctg tcggccacaa cagcagccac ccgctgcccg acgaaccgca
       61 cgacgttatc cagcatgtag gtgtcgtcgg gatcgacgag gtggtcgtcg tggatggcgg
      121 tggtatacag gcgtctgggc acgtcctccc aggtgaagac ctcatggaca ccaggcgtcg
      181 ctagagcggc ctccttgcgg atagcgacga tgcgggcgtg ggcgtgcggc gacctgagca
      241 ccttcagatg cagcaggtcc tcgatctcgg tatccagggt gtagcgtacc ttccccgtta
      301 cgatccccac gctcgccggg gctggcaggt tcgctccgca ggccgcgcca ggctggtcgt
      361 cgtcgataga tttcctcccc tggatcgcat cctcgatcgc acggtaccct gtacagcggc
      421 acaggttgcc cttcaagtaa cgtgggagat cctggcgttc ctcctcgttc aggtttgacg
      481 acaccatgat cattccagca gtacagaacc cgcactgaaa gccctgggat tcgataaacg
      541 accgctgcat ggggtggagc gagccatcct tggcaagacc ctcgatcgtg gtgacccggc
      601 gaccttcagc gcggaaggca ggaatgatac agctgttgac ggtaaccccg tcgagccaga
      661 cggtgcacgc cccacaatcg ccggtgtcac atcccttctt tacgccgaac caacccagtt
      721 cgcgcaggaa ggtgcgcaag cattggcctg gcgccggttc gttcgagaac gtcttgccgt
      781 tgacgtgata gctcatgcgc aactcctcac gcggcctggg agagatcgcc gcggatctcc
      841 tctgcaaagt ggtaggtcat gtgtttgcga tagtccggcg ctccgtggac gtcatcgtga
      901 tacatggagt cggcgatggt ctcccggagg cgctcgcgaa ggcttctcgc atcaggcacc
      961 gcatcgaagc tcagccgtac cggcctaacc gtcgatgcgg agacggtgag catgaatgtg
     1021 ccgtcctgcg gggatagcgt gccgatgagg agcgcggtcg agcggccaag gtgggtcagc
     1081 gagatgcggc ggaagcacgt tcgctttcgc aaagcggcaa tcggcaactc tatcgaacgg
     1141 agcaagtcgc ccggttggag gatgttctga tgatcgcccg tgacgaactc atcagcggcc
     1201 acacgacgct cgctgccgtc ccgcaacctg agtgtgtaga cgccttcgag cgcgaccgtc
     1261 agcgagatca ttggacctgc tggcaaggac atgcagatgt tgccgccaac ggtggccgtg
     1321 ttccaaacct tgaatgacgc caggaacgcg tggcaacact gttggatcag cggcgccgcc
     1381 gtccactccg gaggcgcttc caggcgctcc acctgcgcga tcgtgcagat gggggatatc
     1441 cggagtccct gctcaccgac caccagcggc tcccagttga gtccatgcaa atccagcagt
     1501 cggcgcagat ggggctgcgg ttcggagaag agccaggtgc cgccggcgag ccagctgtct
     1561 ccttcccgcc actcgaaatc ctcatcccct tctccggtta cccgcctgac gtcggtgatg
     1621 gtgttcaggt tcaacttaca agtcctcctc gcattgcagc cgccaacaac tccgtcgtga
     1681 acatcaacat caggtagccg acgcctcccg ctaacaactt ccgactcccg ctcacggctt
     1741 caactccttt tcaggtaagg ccgtaggaaa gagtggaccc tccgcgccgt aacgcaaggg
     1801 tagcttattg ggccggcagg tgatggcgca cccccaaaat ggactagttt tgaatctttc
     1861 ttgtagaacc tccgagaaca gataccgtca ctccgacaag catatagatt tacggtcgtc
     1921 tcgctccgtt cgtacttggg ttgtgcagca acccgagtac atctccttac ctattgggaa
     1981 gaacctcaaa agctgaatgc cgtcatttgc aggaactttg caagctctgc acctcttgca
     2041 aactctcacg ctgactcgac acggcacagg tcgctggttc ggcaggtcgt cgcccaccaa
     2101 gagatacctg caaatgatgg gaagcgagga gccctggttt tcgctccgtg gggcttttgc
     2161 tgcaacctta cagttacgcg accggttcaa ttggcaacgg tcccgtcgcc cacgatcctt
     2221 gcctcatcac ccgcccctgt atcagtgggt gtagggttgc cgtaaatacg cccggatcca
     2281 ggtctcccgg ccctgtccta catggcctga agaaaaacgg cacctccggt ttgacgcgtg
     2341 accgggcgcg aacttgtgcg tctttgtgtc cctatcatct ctcacggctc attgaagcct
     2401 ggagaagaac gctggctcag cgccttttcc aaagattcct tgtcagtctg caccgcagcg
     2461 cgtcgatcgc cgccaagggc ctgtgcagct ccactcttgc cgattcgtat ttttcttcca
     2521 tcctttgatg tcgccagaga tctatccaag caggtccggt cgtatagagc ggtttgcgga
     2581 aagagtgaac ctgaggtgag taaaggtttc aggctacagg catcagcaaa agacctgtag
     2641 cctgatgcct tctccgaagg agtagctcat ccttttcgag aactgtcata ggcatgcggc
     2701 cgccaaagct actctgggcc ttctcgtcgc ctactggtcg gaggtccccg gaacacgtcc
     2761 agtgtgatga aaacacctcc catgatgaat agcagcgctc caaggacaac aagcaggttc
     2821 ggttgtggct tgataaagct ggagacgagc ggagcgtgaa tgcccagggc gagcgcagcc
     2881 cccttgcgca agatcccgga gcgccagatg gcgacaccga agagaatgaa gccggtcgca
     2941 tagagcgcga aaacagggat atagatcgca ttcctcaatg gattgccgaa cagggcgtca
     3001 cctattgcct ttgcatcttc ctggccattt agataggcgt ggccaagcac cggaagggta
     3061 taggtagtaa cgcccagagc cggcaggacc agtgcgaggc tgaggatact caagatcata
     3121 gcggccacgg ccagacttct cgctcgggag tttgccaggt aggccgtcag ggcgaagatg
     3181 ccgaggatca agagggccag gccccctatg ttgccaacga actggctcac gataaagctg
     3241 gttgaactgg ctaccttcgc ggctgcctcg aaatcgacgc gtgggttggg cgcggtgtat
     3301 ctaccaagca ggccccatag cgtcagcaat ccggacaggg gcaaggcaag gataccgagg
     3361 cgcactaggt tgttggtttg tctacggttc acagatcctc gctctcgtcc gccgaatatc
     3421 ccacattcct aagtagttct tcccagcgcc ctgtcgaggt tgaatgcggc gctgatgaga
     3481 gccaggtgag tgaaggcctg tgggaagttt cccagaagct cgccggtcgg gccgatctcc
     3541 tcggagtaga gtccgaggtg gttggcgtag gagagcattt tctcgaaggt gagccgagcc
     3601 tcgtcgagcc gtccggcgcg ggccagtgcc tcgacgtacc agaaagaaca tatggagaac
     3661 gtgccctcgt cgcctgccag gccgtcgggg gaagcggccg ggttgtaacg atagacgagg
     3721 ctgtccgaga ccagttcgtc cgagatcgca tcgagggtcg agagccagcg cgggtcggtc
     3781 ggggtgatga acttgaccag cggcatgagc agaacggagg cgtcgaggac gtccgtatcg
     3841 tagtgctgca cgaacgccct gcgttgctcg ttccagccct gacgcatgat ctggcggtag
     3901 atgcggtcgc gctcggggat ccaacgcgcc aggttg
//
LOCUS       DATAIR010000382        12264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_393854, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000382 DATAIR010000000
VERSION     DATAIR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393854"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1208
                     /locus_tag="VHM69_09345"
     CDS             93..1208
                     /locus_tag="VHM69_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740640.1"
                     /translation="MGVRERVRSFVHERIKPNIKEWYDEAVFPRDIVPGFAELGLLGM
                     HLSGYGCAGKSAVEYGLACMELEAGDSGLRTFVSVQGSLAMSAIHKFGSEEQKQEWLP
                     PMARGEAIGCFGLTEPTAGSDPASMKTFARREGSDWVLNGEKRWIGLATVADVAVVWA
                     RTDEEDNPVRGFLVPTDSTGFSAKDITPKLSMRASIQCDIRLEDVRLPESAMLPEARG
                     LGGPFACLGEARYGIIWGSMGAARDCFESALAYAKEREQFGKPIASFQLVQQKLANML
                     IEVGKGTLLALHIGRMKDAGTLRPEQISFGKLNNVREAIEVAREARTVFGGNGVTLDY
                     PPMRHMSNLESVRTYEGTDEVHTLVLGGAITGIPAYR"
     gene            1251..2225
                     /locus_tag="VHM69_09350"
     CDS             1251..2225
                     /locus_tag="VHM69_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX2740641.1"
                     /translation="MKAILVKEFGEPDVLRYVDVERPSPGEGEALIEVRSAGVNYADT
                     MRRRNQYVVSQDLPFIPGAEVAGTVAEVGEGVEDVAVGDRVVTLLGMGGYAEYAVAPA
                     RNLIPLPEGLDFDNAAAIPLQGLTAYHCIKTSGALKEGESVLVHAAAGGVGTLSVQMA
                     KLLGAGIIIATASSKEKLELARSLGADVLIDYTEEDWPEGVREATEGKGADVILEMVG
                     SDFPEKNLQCLNVFGRMVVFGAASRERGTIVPASLMRRCHAVVGFYLPQVMRRPDLFV
                     PSLREVLGWISSGKLKLTIGGTYPLAQAAEAHTALEGRQTTGKILLNP"
     gene            complement(2344..3111)
                     /gene="fabG"
                     /locus_tag="VHM69_09355"
     CDS             complement(2344..3111)
                     /gene="fabG"
                     /locus_tag="VHM69_09355"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX2740642.1"
                     /translation="MRLDGRTAVVTGAARGIGAAEAIRLAQEGANVAVLDLSAEACRE
                     TVEAVEAAGVEALAIACDVSSAQQVEKAFEEIANRFGRVDILVNNAGLLRDNLSFKMS
                     EDDWDKVLDVHLKGSFLCSKAAQKYMVDQEYGKIVMTSSIVALGNKGQANYSAAKAGL
                     QALARTLALELGRFNVNVNAVAPGWIETEMTKEAAERVGMTMEDMKERFAKNIPLKRF
                     GRVEDIANVVAFLVSDDASYISGETIYIAGGPSSSVI"
     gene            complement(3386..4135)
                     /locus_tag="VHM69_09360"
     CDS             complement(3386..4135)
                     /locus_tag="VHM69_09360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX2740643.1"
                     /translation="MGTLKEAVVVVTGASRGLGRAIAEELASGGAKVVVNYSRSKEPA
                     EEVVNNISENGGEAIAVQADVSDAEQAQKLIDQAIEKFNRVDILVNNAGINIDRTLKK
                     LSVEDWDKVIQVDLNSAFYTVHAVLPHMTEQGGGKIINMSSFVGEAGNIGQANYSAAK
                     AGLLGFTKTAAQELARVGITVNAICPGFIETDMVANIPEEAQQKLRKQVPLGRFGKPE
                     EVARAVRYLIVDGDYITGQSIDINGGIYIRT"
     gene            4643..5056
                     /locus_tag="VHM69_09365"
     CDS             4643..5056
                     /locus_tag="VHM69_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2740644.1"
                     /translation="MNNEGQERKVEGQERVLRGVEARPRNWLVPVILLSLREWNSYGY
                     ELMERASAFGFEAMNPGTLYRTLRQMEKDGIVESSWETSRGGPARRMYTITDAGKSYL
                     DFWAKSLEQYQQTMDNFFRLYTGRPLQENEEKKDE"
     gene            5098..5430
                     /locus_tag="VHM69_09370"
     CDS             5098..5430
                     /locus_tag="VHM69_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740645.1"
                     /translation="MATTQKANKAVERLAETTRDSYQTVVDHAVGLQERNIRFAQGVM
                     DGTIKELRFQAESNRNVAGELFERAEDQRDAYQTLVEQSVDAYMDLAYAPFTYYKEGL
                     EAARKATR"
     gene            5556..5897
                     /locus_tag="VHM69_09375"
     CDS             5556..5897
                     /locus_tag="VHM69_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740646.1"
                     /translation="MDQKQQKQVNEAAEKFAAALKESYQSIADRSVSAQELNAQLTQD
                     FFNGVINNLRTQAENNRALSEDLIEQQRKQQEASQALAQESVNAYMDFLNSMFAYYRG
                     NLDEMKRKASK"
     gene            6003..7214
                     /locus_tag="VHM69_09380"
     CDS             6003..7214
                     /locus_tag="VHM69_09380"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2740647.1"
                     /translation="MSFGNGNGNGEIVISTPLRTAIGTFGGALKDTPATDLGATVGKE
                     VLSQAGVEGDQVDQVIVGNILSAGQGMNPGRQVGIKSGLPVEVPGMTLNRMCGSGLQA
                     VISAAQEIALGDADVVMAGGIENMDQAPFLMPKGRYGYRMGMPDAKIYDHMVYDGLWD
                     VFNNYHMGVTAENVAEKYGITREESDSYAVRSHQRAAKAHEDELFDGQIVPVEVRQKK
                     EKVPFTTDEHIRANASVEGLQKLKPVFKKDGGTVTAANASGINDGAAMMIVSSERKAE
                     ELGMPVAGRLVSAAVAGVDPSVMGTGMIPASRLALKKAGLSVDDLDAVEANEAFSSIA
                     IAVGRELGVPEEKLNPVGGAVALGHPIGATGAVLTVKILHHLARTGGQYGLVTLCIGG
                     GMGIAAIFERV"
     gene            complement(7293..7817)
                     /locus_tag="VHM69_09385"
     CDS             complement(7293..7817)
                     /locus_tag="VHM69_09385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740648.1"
                     /translation="MAIDGHVALALRDGTSVQVRRIRAEDAAALQRLVGRSSDRSIEL
                     RFFGPLKALSDEMAERFADIDGKDRFAAVALDPQDPGEIVAVVRYGREEGTDSAEYAA
                     LVEDRFQGKGLGIGLTRHLIEAARENGIRHLHAMVMRENIGMLSLLRSLDLPERERWQ
                     DGAEHVEIDLEPAA"
     gene            complement(7931..8362)
                     /locus_tag="VHM69_09390"
     CDS             complement(7931..8362)
                     /locus_tag="VHM69_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740649.1"
                     /translation="MSESRKETARAELRISPEDARSIASLLRYTISLNTTAGLVSPTQ
                     RRLATSTYKAVELAALVLEGKPLDEAIRESDQTWTGSLEEDHLRAIELLERERDALRD
                     VLSNHLSPEQLADYLEVKEEQFLELLRQKPAKQKDTDTPGG"
     gene            complement(8367..9638)
                     /locus_tag="VHM69_09395"
     CDS             complement(8367..9638)
                     /locus_tag="VHM69_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740650.1"
                     /translation="MTPGIGEKEEPESMTFTAEEMNRERGPVDLIDANRLLSDEERGV
                     RDRVREFVDREVIPTAADYWDRAEFPFGLLPGLGELGLMGGTISEEYGCAGWNNVAYG
                     LAIAELARGSGSLATFLHVQSGLAMTAIHELGSEEQKQRWLPPMAKCEKVGCFGLTEP
                     EAGSDPGSLTTTATERDGGYILNGEKKWIGNASFCDVAVIWARTPEGKISGFLVESDN
                     PGFSADVLPRKASQRAAWQTHITLEDCHVPENARLPKADGLGSTLSILTHSRYGVGWD
                     GLGQASDCYETALAYAKEREQFGQPIASFQLIQQKLVEMVNEISLSQLLSIHVGRLKD
                     AGDLDPATVSMFKMNNVAKARRTAALARDVLGGNGILLDYRVMEHMADIEGVYTYEGT
                     NDVNTLIVGQAITGHRAFSPRPPQRAEEGTE"
     gene            9879..10901
                     /locus_tag="VHM69_09400"
     CDS             9879..10901
                     /locus_tag="VHM69_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2740651.1"
                     /translation="MPVTITENTSIDYDISGEGPTLLLIGGLGFGRWGFFKQVPAFSR
                     RFRTITFDVRGERRLQNGVADLVDDVVALLDHLGVKKAHVLGTSLGGFVAQGLALMRP
                     DLVDRLVLVCTSYGGRGPESMSFQALADMMGWGSIDPEAVVRRGLETATSETYRAEHE
                     DEFEQIVRWRLADSPSEAAYHEQARAGAGFDLSGDVGHITSPTLVIHGAEDRYVPVAN
                     AAALAGAIPDSRLRVLDDAGHLVFIERSTEVNREVVSFLKPRRPYRPRTYIQRPKWPA
                     KVQGWLRRVPRSIGRAVRLDVGQHHLRSLAGHFRNTGKMGINKVAEYKRAAEAARRNS
                     LRRKTR"
     gene            10898..>12264
                     /locus_tag="VHM69_09405"
     CDS             10898..>12264
                     /locus_tag="VHM69_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX2740652.1"
                     /translation="MSEGKTSNGGAEIPMAPFDVWTEWMQSNMGPMMATPGASVPWLM
                     APPGVRTGEEAADIPEGAMRNDPLLSTVEKLWDANPVQNVLPINWVEITRSLQTLWAR
                     ELSDPARAMERATDFNAKLFGATMRSWQDATSRFWGLETPQEEEEGRPDKRFSAPDWE
                     QNPFYETLKESYLLASEYLLNEAEETDDEDTPEQRRLKFHLKQFVDAMAPVNYLLTNP
                     AALKRAFETGGASLAEGTRNLLADLQEGRMSMTDANAFQPGENLAITPGKVVYRNELI
                     ELIQYEPTTEQVHETPVMFIPPWINKYYILDLQPKNSFVKYLVDEGYTVFMVSWKNPD
                     ASLEDTKFEDYMTKGPLAAAEAVRDITGAEKVNPVGYCVGGTLLAIALSWLAAGDDEN
                     PFGPPTFMVALHDFTEVGDTGVFIDEPQIEFMEQQMLERGYLDNRKMVNMFNLLRSND
                     LIWSNV"
BASE COUNT         2400 a         3723 c         3833 g         2308 t
ORIGIN      
        1 cgaaggcagg gagaaacgcg gatgccggcg ctttcggtac tcccgaggcg ggcgacttcc
       61 tgaacatcga cgcccacctc tccgaagagg agataggcgt cagggagagg gtccgctcgt
      121 ttgtgcacga gaggatcaaa cccaatatca aagagtggta cgacgaggcc gtcttcccga
      181 gggacattgt gccagggttc gcggagcttg gcctgctcgg gatgcacctc tccggctacg
      241 ggtgtgcagg gaagagtgct gtcgagtacg gactcgcgtg catggagctc gaggccggcg
      301 actcgggact caggaccttc gtcagcgtgc agggatctct cgccatgagc gccatccaca
      361 agttcggctc ggaggagcag aaacaggagt ggctgccgcc gatggcgagg ggagaggcta
      421 tcgggtgctt cggcctcacc gagccgacag cgggctccga tccggcgagc atgaagacgt
      481 tcgcccggag agaagggtcc gactgggtct tgaatgggga gaagcgttgg atcgggctcg
      541 ccaccgtcgc cgacgtcgcc gtcgtctggg cgcgcacgga cgaagaggac aaccccgtcc
      601 gcggctttct ggttcccacg gactccaccg gcttctcggc gaaggacata acccccaagc
      661 tctcgatgcg ggcttcgatc cagtgcgaca tacgcctcga agacgtccgc ctccccgaga
      721 gcgcgatgct gcccgaggcc cgcggactcg gcggaccttt cgcctgcctg ggcgaggcca
      781 ggtacggcat catctggggc tcgatggggg cggcgaggga ttgcttcgag tccgcgctgg
      841 catacgcaaa agaaagggag cagttcggca agcctatagc cagcttccaa ctcgtccagc
      901 agaagctggc aaacatgctc atcgaggtag gcaagggtac gctcctcgcg ctccacatag
      961 gcaggatgaa ggacgccggc acgctcaggc ccgagcaaat aagttttggc aagctcaaca
     1021 acgtcaggga ggccatagaa gttgcgcgcg aggcccgcac cgtcttcggc ggcaacggcg
     1081 tcaccctgga ctatccaccc atgcgccaca tgagcaacct ggagagcgtg aggacatacg
     1141 aggggacgga cgaggtccac actctcgtcc tcggcggcgc cattaccggc atccctgcct
     1201 accgttagta tggccttatc aagtactgaa ttggaggcgc aatggcgcag atgaaggcga
     1261 tattggtcaa ggagttcggc gaacctgacg tcctgaggta tgtagatgtc gagcgtcctt
     1321 cgcccggcga gggggaggcc ctgatcgagg tccgctccgc aggcgtgaac tacgctgata
     1381 ccatgcgccg caggaaccag tacgtggtct cccaggatct ccctttcatt ccaggagccg
     1441 aggtggctgg caccgtcgcc gaggtggggg agggggtcga agacgtcgcc gtcggcgacc
     1501 gcgtcgtcac cctgcttggt atgggcgggt atgcggagta cgccgtcgcc cccgcgcgga
     1561 acctgatccc actccccgaa ggtctcgact tcgacaacgc cgcggcaatc ccactacagg
     1621 gcctgaccgc ctaccactgc atcaagacct caggcgccct gaaggagggc gagagcgtcc
     1681 tcgtccacgc cgccgcaggg ggcgtcggca ccctgtccgt ccagatggcg aagctcctcg
     1741 gtgcaggcat catcatagcc accgcaagca gcaaggagaa actcgagctc gcgcgctccc
     1801 tcggcgcgga cgtcctcatc gactacacgg aggaagactg gcccgaaggg gtgcgcgaag
     1861 ccacggaagg caaaggcgcc gacgttatcc tcgagatggt cggaagcgat ttccccgaga
     1921 agaacctgca gtgcctgaac gtcttcggca ggatggtcgt cttcggcgcc gcgagcaggg
     1981 aacgaggaac gatagtgccc gcctccctta tgaggcggtg ccacgccgta gtcggtttct
     2041 acctgccgca ggtgatgcgc cgtcccgacc tcttcgtccc cagcctccgg gaggtactcg
     2101 gttggatctc ctcaggcaag ctgaagctca ccatcggcgg cacctacccg ctcgcccagg
     2161 ccgccgaagc ccacacggcc ctcgaaggcc gccagaccac aggcaagatc ctgctgaatc
     2221 cataggcttc aggcatcagg tcctcgctcg gcggagcgtc cgagcgtagg gcaatctcca
     2281 cgggcagcga aaagggcggg gagaaatccc cgcccaaaac ctgaagcctg ttgcctgaag
     2341 cctctaaatc accgagctcg aagggccgcc tgcgatgtag atcgtctcgc cgctgatata
     2401 cgaggcgtcg tctgatacga ggaaggcgac gacgttggct atgtcctcta ctctcccgaa
     2461 acgcttgagg gggatgttct tggcgaagcg ctccttcatg tcctccatcg tcatcccgac
     2521 gcgctcggct gcctccttgg tcatctcggt ctcgatccaa ccaggcgcca cagcattgac
     2581 gttgacgttg aaccgaccga gctcgagcgc gagcgtccgc gcgagggcct gcaatcccgc
     2641 cttcgcagcg gagtagttgg cctgcccctt gttcccgagc gccacgatgg agctggtcat
     2701 cacgatcttg ccgtactcct ggtcgaccat gtacttctgc gccgccttgg agcacaggaa
     2761 actgcccttg aggtggacgt cgagtacctt gtcccagtcg tcctcactca tcttgaatga
     2821 gaggttgtcc cgcaagagcc ccgcgttgtt gacgaggatg tccacccgcc cgaagcggtt
     2881 ggcgatttcc tcgaaagcct tctcgacctg ctgcgcactc gatacgtcgc aggcaatggc
     2941 cagggcctca acaccggcag cctcgacggc ttcaacagtc tcccgacaag cctcggccga
     3001 aagatccagg accgctacat tcgccccctc ctgcgcaaga cggatcgcct ccgctgcccc
     3061 aatgccgcgc gccgcccccg ttacaaccgc cgtacgtccg tcaagcctca agttccacct
     3121 ctctcgttag ccggacatcg caactctcac cccgcagggg tgttgtcttt gttacatgac
     3181 agcgaagtaa ttgtatacga attgaacgta tgtgtccaga acagatatgt gcgaggagtg
     3241 gaacgtattc tagaatacaa ctccacgggc aggattatat gcttttgcgg gcgttcaggc
     3301 tacgtgtagc gtcacatgtc cggtgacagc aaaaagcccc cgcctgtaag tggggcgggg
     3361 gcaacgggag cgagaacttc cttccctatg tcctgatgta gataccgccg tttatatcga
     3421 tagactggcc tgttatgtag tcaccgtcca ctatcaagta gcgaacggcg cgggcgacct
     3481 cttccggctt cccgaagcgg ccgaggggga cttgtttgcg gagcttttgc tgggcttcct
     3541 ctgggatatt ggcgaccata tccgtctcta taaagcctgg gcagatagca ttcacggtga
     3601 tcccgacgcg ggcgagttcc tgggctgcgg tcttggtgaa gccgagcaga ccggccttgg
     3661 cggccgagta gttggcctgg ccgatattgc ctgcttcgcc gacgaaggag ctcatgttga
     3721 tgatcttacc cccgccctgc tccgtcatgt gcggcaatac ggcgtggacc gtatagaagg
     3781 cgctattgag atctacctgg atgaccttgt cccagtcctc gacggagagc ttcttcagcg
     3841 tgcggtcgat attgatcccc gcgttgttga cgaggatatc caccctgttg aacttctcta
     3901 tcgcctgatc tatgagcttc tgggcctgct cggcgtcgga gacgtctgcc tggacggcga
     3961 tggcctctcc accattctcg gagatgttgt tcaccacctc ttcggcaggt tccttgctcc
     4021 tggagtagtt gacgactacc ttggccccac cgctagccag ctcctcggct atggccctgc
     4081 ccagacctct gctcgcgccg gtgacgacaa cgaccgcttc tttcaacgtg cccatgctga
     4141 tctccctttc ccgattgcct ttgaatagag tttattgaca gaagaattca ggcgcaaact
     4201 agataccttg cgcacgtaag taccggccct cactcgccac cattcatcgg cgggaacata
     4261 taccccgtga atccaggcca ttgccgtagg ctcgaaggat gatccgcgac aggctacggt
     4321 gtgacatctg ttcggaagag aggagatcag gagcccacgc actccggcga cgtgcccgtc
     4381 tgaaggccag gtccggagtc cccgttcgct ccacaagcga agcaggaaaa taattgaggt
     4441 ctttaccggg gtgctctgtc ataatatgtg cgtgttgact agagccacgc acgacctaag
     4501 tgtcgatccg acttctggtg tgggatcggg ggttgaacgt aagactctct gtctgcagaa
     4561 tttttcggtg cagggcccgt ctattacctt gacggataga aacatatatg taaaatgcac
     4621 atagagtatg gaagagagag ccatgaacaa cgaaggacag gagcgcaagg tcgagggcca
     4681 agagcgagta ttgcgtggag ttgaggcgcg gccgaggaac tggctggtgc cggtgatcct
     4741 gctatctttg cgggagtgga actcctacgg gtacgagctc atggagcggg cttcggcttt
     4801 cgggttcgag gcgatgaatc ctggaacgct ttaccggacg ctacggcaga tggagaagga
     4861 cggcatcgtc gagtccagtt gggagacttc gagaggtggg ccggcacgtc ggatgtacac
     4921 catcaccgac gcggggaagt cgtatctgga cttctgggcc aagtctctgg agcagtacca
     4981 gcaaaccatg gacaacttct tccggctcta cactggcagg ccgctgcagg aaaacgaaga
     5041 gaaaaaggac gagtagcggc gagcaagttc gcgtatgacg cgagaaagga gaagtagatg
     5101 gctactacgc agaaggcaaa caaggcggtt gagaggctcg ctgagaccac gcgggattcc
     5161 taccagacgg ttgtggacca cgcggtgggt ctgcaggagc ggaacatccg gttcgcgcag
     5221 ggagtaatgg acggcacgat caaggagctc cgcttccagg cagagagcaa ccgcaacgtt
     5281 gctggcgagc tattcgagcg cgccgaggac cagcgcgacg cgtaccagac cctcgtcgag
     5341 cagtccgtcg acgcctacat ggacctcgcc tacgccccct tcacctacta caaggagggc
     5401 ctggaggccg ccaggaaggc caccaggtaa ggcgtagcac cacgtcgaag tcttcggggc
     5461 ggaccttcgg gtccgcccct ttgttttatg tttcgctgca ctgatatcgg gtaacaacaa
     5521 gtgtgtacgc tagctcaact agaggaagtg aaaccatgga ccagaagcag cagaagcagg
     5581 tgaacgaagc ggcggagaag ttcgcggccg ctttgaagga atcctaccaa tcgatcgccg
     5641 accgctctgt ctcggcccag gagctaaacg cccaattgac gcaggacttc ttcaacggcg
     5701 tcatcaacaa cctcaggacc caggccgaga acaacagggc tctgtctgag gacctgatcg
     5761 agcagcagcg caagcagcag gaagcctccc aggcgctcgc ccaggagtcg gtgaacgctt
     5821 acatggactt cctgaactcc atgttcgcct actacagggg taacctggac gagatgaagc
     5881 ggaaagccag caagtaacag aagtgcagcg agttgtggag tttgcagaga ggcccgggcc
     5941 gcatggtccg ggccttgtct tttgatgtat cctcttaata agtgtagacg gaaggagaaa
     6001 aggtgagctt cggtaacggt aacggaaacg gcgagatagt catctcgacg cctctaagga
     6061 cggcgatagg caccttcggc ggcgcgctca aggacacgcc tgcgacggac ctcggggcga
     6121 cggttggcaa ggaggtactc tcccaggctg gcgtcgaggg agaccaggtg gatcaggtga
     6181 tagtcggtaa cattctctcc gccggtcagg gtatgaaccc agggcgccag gtcggcatta
     6241 agagcggtct ccccgtcgaa gtgcccggga tgaccctgaa caggatgtgc ggctcggggc
     6301 ttcaggccgt aatctcagcg gcgcaggaga tagcccttgg cgacgccgac gtagtcatgg
     6361 ccggcggcat cgagaacatg gaccaggcgc cgttcctcat gcccaaaggc cgctacggct
     6421 acaggatggg gatgccggac gccaagatct acgaccacat ggtctacgac ggcctgtggg
     6481 acgtcttcaa caactaccac atgggcgtga cggccgagaa cgtcgccgag aagtacggca
     6541 ttacgcgcga ggagtccgac tcctacgccg ttcgaagcca ccagcgggcc gccaaggctc
     6601 acgaagatga gctattcgac ggccagatcg tgcccgtcga ggtcaggcag aagaaggaga
     6661 aggtcccgtt caccacggac gagcacatcc gcgcgaacgc gagcgtggag ggccttcaga
     6721 agctgaagcc agtcttcaag aaggacggcg gcacggtaac cgccgcgaac gcttcaggca
     6781 tcaacgacgg agcggccatg atgatcgtga gcagcgagcg caaggccgaa gagcttggga
     6841 tgcccgtggc gggcaggctg gtctccgcgg ccgtcgctgg cgtcgacccg tcggtaatgg
     6901 gtactggtat gatccccgcc tccaggctgg ccctgaagaa ggcaggtctc tcggtggacg
     6961 acctcgacgc cgtcgaggcg aacgaggctt tctcgtccat agctatagcc gtcggacgcg
     7021 agctcggagt gcccgaggag aagctcaacc ccgtcggcgg ggctgtggcc ctggggcacc
     7081 cgatcggggc cacaggcgcc gtccttaccg tcaagatact gcaccacctc gcccgcaccg
     7141 gaggccagta cggtctggtg acgctctgca tcggtggcgg catgggcatc gccgctatct
     7201 tcgagcgcgt ctagtagaga cgtcggatca ggtagcgaca gaggagcggc cttcgggtcg
     7261 ctcctctgtc gtcaatactc ctgcaattca ctttaggcgg cgggttcgag gtctatctcg
     7321 acgtgttcgg cgccgtcctg ccagcgctcg cgctcgggga gatccaggct cctcagcagg
     7381 ctcagcatcc cgatgttctc ccgcataacc atcgcgtgga ggtgccttat cccgttctcg
     7441 cgggcggctt cgatcaggtg tcgcgtcaac ccgataccaa gtcccttccc ctggaacctg
     7501 tcctcgacga gcgccgcgta ctcggcactg tctgtgcctt cctcccgccc atagcgcacg
     7561 actgccacga tctcaccagg gtcctgcgga tcgagcgcca ccgccgcgaa cctgtccttg
     7621 ccgtcaatgt cggcgaaccg ctcggccatc tcgtccgaga gcgcctttaa gggaccgaag
     7681 aaccgcaact ctatggaacg gtcgctcgat cggcccacga gccgctgcaa cgccgcggcg
     7741 tcctcagccc gtattcgacg tacctgtaca ctggtcccgt ccctcaaggc cagggccacg
     7801 tgcccgtcta tcgccacggg gaccccggat caccggctat ctcatgtcta tgtgcagagt
     7861 gcatataacg aacagtctac cgcgctctgg ggatgcgaca atagcggggg cgcggttttg
     7921 tgttagagag ctaacctcct ggagtatctg tgtccttctg tttggcgggt ttctggcgga
     7981 ggagttccag gaactgttcc tctttgactt cgaggtaatc ggcgagttgt tcggggctca
     8041 ggtgattcga caggacatct ctgagggcgt cgcgctccct ttcgaggagt tcgatggcgc
     8101 ggaggtggtc ttcttcgagg gagcctgtcc aggtctggtc cgactcccgg atcgcttcgt
     8161 cgagcggctt tccttcgagg acgagggcgg cgagctcgac ggccttgtag gtcgaggtgg
     8221 cgaggcggcg ctgggtcggg gagacgagcc cggctgtagt gttcaggctt atggtgtagc
     8281 ggaggaggct ggcaatgctg cgggcgtctt cgggtgagat ccgcagttcc gcccttgcgg
     8341 tttctttcct gctttctgac acgtttttac tctgttcctt cctcggcgcg ttgcggtggt
     8401 cggggagaga aggcgcggtg gcctgtaatc gcctgtccca caatgagggt gttgacgtcg
     8461 ttggtgcctt cgtaggtgta gacgccctcg atgtccgcca tgtgctccat tacgcggtag
     8521 tcgagcagga tgccgttgcc gcccagtacg tcgcgggcga gtgccgcggt acggcgggcc
     8581 ttcgcgacgt tgttcatctt gaacatcgag actgtggccg ggtcgagatc gccggcgtcc
     8641 ttgaggcgac ccacgtggat ggagaggagc tggctgagag agatctcatt gaccatctcg
     8701 acgagctttt gctggatgag ctggaatgaa gctatgggct gcccgaattg ctccctttcc
     8761 tttgcgtaag ccagcgccgt ctcgtagcag tccgacgcct ggccgagacc gtcccatccc
     8821 acgccgtatc gcgagtgcgt gaggatcgag agcgtcgagc caagtccgtc ggcttttggc
     8881 agtcttgcgt tctcgggtac gtggcagtct tcgagggtga tgtgcgtctg ccaggcggcg
     8941 cgctgggagg ccttgcgggg gaggacgtcg gcgctgaagc ccggattatc ggactcgacg
     9001 aggaagccgg agatcttgcc ctccggggtc ctggcccaga tcacggcgac gtcacagaag
     9061 gaggcgttgc cgatccactt cttctccccg ttcaatatgt agccaccgtc ccgctccgta
     9121 gcggtcgtcg tgagagagcc tgggtcgctc cctgcctccg gttctgtgag gccgaagcag
     9181 ccgaccttct cgcacttggc catcggcggc agccagcgct gtttctgctc ctccgagccc
     9241 aactcgtgga tagccgtcat cgcgagcccg ctctgtacgt gcaaaaaggt ggccagactc
     9301 cccgatccac gagcaagctc cgcaatcgcg aggccgtatg cgacgttgtt ccagcccgcg
     9361 cagccgtact cctccgagat ggtccctccc atgagcccga gctccccgag cccagggagg
     9421 agcccgaacg ggaactctgc cctgtcccag taatcagccg ccgtcgggat gacctcgcgg
     9481 tcaacgaact cgcggaccct gtccctgacc cctctctcct catccgagag caacctgttc
     9541 gcgtctatga gatccacagg ccccctctcc cgattcatct cttctgccgt gaaggtcatg
     9601 ctctccggtt cctctttctc tccgattcca ggcgtcatcc cgctctcctc tgctagcaag
     9661 aataggatct tgcgacgacc cggtcaatct accagtgatg gctttcacgt acccgccttg
     9721 ctcatcttac cgggccatct acaataatga tcgttgcacc taagtgcaga acgcatctac
     9781 ctttccagtg agaagttctg gcctggcatg agacgtggcg caggctgcca gatctctcgt
     9841 cgggcgactc acaggagact gagggaggcg agcaggcaat gcccgtgacg atcaccgaga
     9901 acaccagcat cgactacgac ataagtggag aggggccgac gctgctcctt ataggtggcc
     9961 ttggcttcgg tcgctggggg tttttcaagc aggttccggc cttctcgcgc cgatttcgga
    10021 ccatcacgtt cgatgttcgc ggtgagcgga ggctccagaa cggcgtcgcc gaccttgtgg
    10081 acgacgtggt agcgctattg gatcacctcg gggtaaagaa ggctcatgtc cttggaacat
    10141 cgctcggcgg cttcgtggcg caggggctgg cgcttatgag gcctgatctg gtcgatcgtc
    10201 tggtcctcgt atgcaccagc tatgggggca ggggaccaga gtcgatgtct ttccaggccc
    10261 tggcggacat gatggggtgg ggctccatcg atcctgaggc ggtggtccgt cgcggtctcg
    10321 agacggcgac ctcggagacc taccgcgccg agcatgagga tgagttcgag cagatcgtac
    10381 gctggcgtct cgcggactcg ccctctgagg ctgcctacca tgagcaggcc agggccggcg
    10441 caggcttcga cctctccggc gacgtcgggc acataacctc gcctacgctt gtgatccacg
    10501 gcgccgagga ccgctacgtg ccggtcgcca acgccgccgc cctcgccggg gccatccccg
    10561 attccagact gcgggtcctc gacgacgccg gccacctggt gtttatcgag cggtccaccg
    10621 aggtaaacag agaagtcgtc tcctttctca agccccgcag gccctacagg ccgcgtacgt
    10681 acatacagcg gcctaagtgg cccgcgaagg ttcaaggctg gctcaggagg gtgccgcggt
    10741 ccatcggtcg cgcggtcagg ttagacgtgg gacagcatca tctgagatcc cttgccggcc
    10801 attttaggaa caccggcaaa atgggtataa acaaagttgc agaatacaag cgggcggctg
    10861 aggctgcccg gcgaaactct ctgaggagga agactagatg agtgaaggca agaccagcaa
    10921 cggcggcgcg gaaatcccga tggcgccttt tgacgtgtgg acggagtgga tgcagtcgaa
    10981 catgggcccg atgatggcga cgccgggcgc gagcgtccct tggctcatgg cgcctcccgg
    11041 tgtcaggacc ggagaggagg ccgcggacat ccccgagggg gctatgcgga acgacccctt
    11101 gctctcgacc gtcgagaagc tgtgggacgc caacccggtc cagaacgtgt tgcccatcaa
    11161 ctgggtcgag atcacacgct ccctccagac tctctgggcg cgcgagctgt ccgaccccgc
    11221 gcgcgccatg gagcgggcga cggacttcaa cgcgaagcta ttcggagcca cgatgcgcag
    11281 ctggcaggac gccacgagcc gcttctgggg tctcgaaacg ccgcaggagg aagaagaggg
    11341 ccgtcccgac aagcggttct ccgcgcctga ctgggagcag aaccccttct acgagacgct
    11401 caaggaatcg tacctgctcg cctctgagta tctgctcaac gaggcggagg aaacagacga
    11461 cgaggacacc ccagagcagc ggcgcctcaa gttccatctc aagcagttcg tcgacgccat
    11521 ggcgccggtc aactacctgc tgaccaaccc cgccgcgctc aagcgggcct tcgagacagg
    11581 gggagcgagt ttagctgagg gaacgcgtaa cctgctcgct gatctgcaag aaggcaggat
    11641 gagcatgacc gacgccaacg ccttccagcc tggggagaac ctggcgatca cacctggcaa
    11701 agtcgtctac cgcaacgagc ttatagagtt gatccagtac gagccgacga ccgagcaggt
    11761 ccacgagacg ccggtcatgt tcataccgcc ctggatcaac aagtactata tcctcgacct
    11821 ccagccgaag aacagcttcg tcaagtacct cgttgacgag ggctacacgg tgttcatggt
    11881 ctcgtggaag aaccctgacg cctcgttgga ggacaccaag ttcgaggact acatgacgaa
    11941 gggcccgctc gcggccgccg aggccgtgag ggacatcact ggagccgaga aggtcaaccc
    12001 tgtcggctac tgcgtcgggg ggacgctgct cgcgatcgcg ctctcctggc tcgccgctgg
    12061 cgacgacgag aaccccttcg gcccgccgac cttcatggtc gctctgcacg actttacgga
    12121 ggtaggggac acgggcgtct ttatcgacga gccgcagata gagttcatgg agcagcagat
    12181 gctcgagcgc ggttatctgg acaaccgcaa gatggtcaac atgttcaacc tgctgcgctc
    12241 caacgacctg atctggtcga atgt
//
LOCUS       DATAIR010000383         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_24601, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000383 DATAIR010000000
VERSION     DATAIR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24601"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1171
                     /locus_tag="VHM69_09410"
     CDS             224..1171
                     /locus_tag="VHM69_09410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740653.1"
                     /translation="MLAFTKEDHEVDESSIRNVLRAEMDAGVRVRQMNDADLERILEL
                     RSVVRWSADPRAFDLLRGVRGARWSVAEARDGALAGMVGAVPLGDIGILCHLAVHDEF
                     RSLGLGALLSSWAVTYLRSRGAKKVRLYSTRKAEGLYRSLGFEEVTPRTVYRLQEGPR
                     RLKVPGQVDGHRVETLTFGDLMELYGVDRWSYGADRSALIFATLRLHPGRGLVVRDAS
                     GRIKGYLIRSAIGRTTRIGPYLASTPDVARLLLTHALSATRGAPAQVTVPDPAGCPAH
                     SLLREFGFEGTEDRLLMELGETRNGTGLVHYGTTPYLAT"
     gene            1326..3056
                     /locus_tag="VHM69_09415"
     CDS             1326..3056
                     /locus_tag="VHM69_09415"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2740654.1"
                     /translation="MLFGRTLREAPSGVEVASHGLLLRAGFIRQVAAGIFSYLPLAKR
                     SIGKIENILREEMDAVGGQEVSMPVVQPAAVWERSGRYHTTGQELVRLRDRRNREMVL
                     AMTHEEIVTSLAASEVDSYQRLPRLVYQIQTKFRDDPRPRAGLIRAREFTMKDAYSLD
                     KDEAGLDQQYRTLYEAYFRIFDRCGLPTTSVGADVGIMGGSMAHEFMYLAPGGEDTIL
                     ICDNCGYTANRQVAKFRKSISEGEEQRPIEKIATPGADTIESLARSLDVPKSRTAKAI
                     FLVATDDGSERFVLAVVRGDMELNETKLANAVGAIELRPALREEIQAVGAEPGYGSPL
                     GVDGVLAVVDDAIPASPNVVAGANEEGFHFLNVNYGRDFEADTVADIASAGDGSFCPE
                     CANPMRAERGVEVANIFKLGTRYSGSLGAGFLDRDGERKPIVMGSYGIGVRRLLACIA
                     EEHHDEDGLVWPISVAPYHVHIVASEVGEAADGLYEKLVSAGVEVLYDDRDDSLGAKF
                     KDADLIGVPIRLTITPRSLEKGGVEIKARRDSESHIVPIEETVDAIRREISRLEAGFA
                     EGAAKRPTLD"
     gene            3116..3628
                     /locus_tag="VHM69_09420"
     CDS             3116..3628
                     /locus_tag="VHM69_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740655.1"
                     /translation="MANTEFLFEFREVHAREISRWATSVEEVRRWSGSDTGFPVDVSV
                     FQHWHADPEVNPYVLCDGDAIIGYGEAWIDETEHEVELGRIIVRPESRSRGVGKRFVI
                     LLLERAAQSGFPDAFVRVVPDNHAAIACYRSAGFSLVPESLRERYNRGQPVDYVWMRH
                     PLTDKGSGPP"
     gene            3708..>4091
                     /locus_tag="VHM69_09425"
     CDS             3708..>4091
                     /locus_tag="VHM69_09425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2740656.1"
                     /translation="MFERNQPDIDAYGRRVRAGTCFVCGIVSRDPDFPKHHVVYEDDA
                     VIAFLNRYPTQFGYTLVAPKEHKVQVADDFTMEEYLGLQRVVHRVAEAVREEMGADRV
                     YLLSLGSNEGNAHVHWHVVPLPPGTP"
BASE COUNT          772 a         1217 c         1350 g          752 t
ORIGIN      
        1 cccttccatg cacgcgcatc gtgttgtagc attgcagacg tggaaggaga gcacataagg
       61 ctggctacga gctcctttac cggacggctc cctcgaccgt ccctgccccg accgtccctc
      121 tagggggccg cgctcggggc atccgcgtct cttcaggttt cgcagaaacc gtccacgcgg
      181 aagagtctcc attcatgaat actcaagaga tcgagggaag accgtgctgg ctttcacaaa
      241 agaagatcac gaagtagacg agagttccat acgcaacgtg ctaagggccg agatggatgc
      301 gggcgtccgt gtcaggcaga tgaacgacgc cgacctcgaa cgcatcctgg agctacgcag
      361 cgtcgtgcgg tggtcggctg atccgagggc tttcgacctg ctgcgcggcg tgcgcggcgc
      421 ccgctggtcg gtggcggagg ccagggacgg cgcgctggca ggtatggtcg gcgctgtgcc
      481 gctcggcgac atcggcatcc tttgccatct cgccgtccat gacgagttca ggagcctggg
      541 tctcggcgcc ctgctctcat cctgggccgt aacctacctg agaagccgcg gcgcgaagaa
      601 ggtccggctc tactcgaccc ggaaggccga gggactctac cggtcgctgg gcttcgagga
      661 ggtcacaccg cgcaccgttt accggctgca ggagggtccg cgtaggctca aggtgccggg
      721 gcaggtagac ggccacaggg tcgagacgct cacgttcggg gacctgatgg agctctacgg
      781 cgtggaccgc tggagttacg gtgccgaccg ttcggccctg atcttcgcca cactgaggct
      841 ccatcctggg cgcgggctcg tggtacgtga cgcgtcgggc aggatcaagg gctacctgat
      901 ccggagcgcc atcggccgca cgacccgcat cggcccctac ctggcctcca caccggacgt
      961 tgcccgactc ctgctcaccc atgcgctctc ggcgaccaga ggcgcgcccg cccaggtcac
     1021 ggtccccgat ccggccgggt gcccggcgca ctccctgctg cgggagttcg gcttcgaagg
     1081 cactgaggat cggttgctta tggaactggg cgagacgagg aatggaaccg ggctcgtcca
     1141 ctacggcacg acgccgtatc tggcgactta ggcttcaggt gtcaggttct tgtggatgat
     1201 ctcgtttccc gtagagagga gtcggtttgt cgaatcgttt ggctacagtt agtcatttgg
     1261 aatgggggaa ctccccggta gtcctgacgg ctgaaacctg aggcctgttc accatgcgca
     1321 tgagtatgct atttggcagg acgttgcgcg aggcgccctc cggggtggaa gttgcgagcc
     1381 atgggctctt gctccgggct gggtttatca ggcaggtggc ggcggggatc ttctcttacc
     1441 tgcctctggc gaagaggtct atcggcaaga tcgagaatat cttgcgggag gagatggacg
     1501 cggtgggcgg ccaggaggtg tcgatgcccg tcgtgcagcc cgccgccgtg tgggagagat
     1561 cgggccggta ccatacgacg ggacaggaac tggtcaggct ccgcgaccgc aggaacaggg
     1621 agatggtcct ggcgatgacc cacgaggaga tcgtcacgag tctggctgcg agcgaggtgg
     1681 actcttatca gcggttgccc cggctcgtgt atcagatcca gaccaagttc cgcgacgatc
     1741 cacggccgag ggcgggtttg atccgggcgc gcgagttcac gatgaaggac gcctacagcc
     1801 tcgacaagga cgaggccggg ctggaccagc agtaccgtac gctctacgag gcatacttca
     1861 ggatcttcga ccgttgcgga ctgccgacca cgtccgttgg cgccgatgtc ggcatcatgg
     1921 gtgggagcat ggcccacgag ttcatgtacc tcgcgcccgg cggagaggac actatcctca
     1981 tctgcgacaa ctgcggatat accgcaaacc gccaggtagc gaagttccgt aaatcgatct
     2041 cggaagggga agagcagaga ccgatcgaga agatcgccac accaggcgcg gacaccattg
     2101 agtcgttggc gcgctctctg gatgtcccga agtcacgcac agccaaggcg atcttcctcg
     2161 tcgccacgga cgatggctcc gagcgtttcg ttctggctgt cgttcgcggc gacatggaac
     2221 tcaatgagac caagctggcg aacgctgtcg gggccatcga gctgaggccc gcgcttcgag
     2281 aggagatcca ggccgtcgga gccgagcccg ggtacggctc cccgctcggt gtggatggtg
     2341 tgctcgcggt cgttgacgat gcgattcctg cctcccccaa cgtggtcgca ggggccaacg
     2401 aggagggctt ccatttcctg aacgtgaact acggcaggga cttcgaggcg gatacggtcg
     2461 ctgacatagc ctctgccggg gacggctcct tctgccccga atgcgccaac cccatgcgcg
     2521 ccgagcgggg cgtcgaggtc gcgaacatct tcaagctggg tacgcgctac agcggctccc
     2581 tcggggccgg tttccttgac cgtgacgggg aacggaagcc tattgtaatg gggtcctacg
     2641 gtatcggtgt ccggcggctc cttgcctgta tcgccgagga gcaccacgac gaagacgggc
     2701 tcgtgtggcc catctccgtt gcgccttacc acgtccacat cgttgcatcc gaagttgggg
     2761 aggcggctga cgggctctac gagaagctgg tcagcgctgg cgtcgaggtc ctttacgacg
     2821 acagagacga tagtctgggg gccaagttca aggacgcaga cctgatcgga gtccccatac
     2881 gcctgaccat cacaccccgt tccctggaga agggaggagt agagattaaa gcccgccgtg
     2941 actccgaaag ccatatagtc cccatcgaag agacggtcga cgcgatccgg cgtgaaatct
     3001 ccagattgga agccgggttc gctgaaggag ccgcgaaacg tcccaccctc gattagggaa
     3061 agatatacgc gtcataatta cgcggcgcac caacggcacg aggcgggtgg acggcatggc
     3121 caatacggag ttcctgttcg agttcaggga agtacacgca cgagagatct cccgctgggc
     3181 cacttccgtc gaggaagtgc gcagatggtc agggagcgac accggattcc cagtcgacgt
     3241 gtcggtattc caacattggc acgccgatcc ggaggtgaac ccatacgtat tgtgcgacgg
     3301 ggacgctatt atcggctacg gcgaggcgtg gatcgacgag accgagcatg aggtcgaact
     3361 gggacgcatc atcgtcaggc ccgagagccg gagccgcggc gtaggcaaaa ggttcgtgat
     3421 cctgctcctg gaacgcgcgg cccaatcggg ctttcctgac gccttcgtcc gcgtggttcc
     3481 tgacaaccat gccgccatcg cctgttaccg gagcgccggg ttctcgttgg ttcccgagtc
     3541 cctacgcgaa cggtacaacc ggggacagcc cgtcgactac gtctggatgc gccaccccct
     3601 gaccgataaa gggagcggtc ctccttgaag cgatcgactt taaggtggat gatagggtcc
     3661 gtgtcgagcc gcgtaagaca agttccgatc ctcaacttcg agtgaaaatg ttcgaacgaa
     3721 atcagcctga catagacgct tatgggaggc gagtccgggc agggacctgc ttcgtttgcg
     3781 gcatcgtatc gcgggacccg gattttccga aacaccacgt cgtctacgag gatgacgccg
     3841 tgatcgcctt cctcaacagg tatccgacgc agttcgggta cacgctggtc gcgccgaaag
     3901 agcacaaagt acaggtggct gacgatttca cgatggagga gtacctgggt ctgcagcgag
     3961 tcgtgcatcg cgtggcggag gcggtgcgcg aggagatggg ggccgatcgg gtctatctac
     4021 tctcgttggg ctccaatgag ggcaacgccc acgtccactg gcacgtcgtg cctttgccgc
     4081 cgggcactcc c
//
LOCUS       DATAIR010000384         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_393934, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000384 DATAIR010000000
VERSION     DATAIR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393934"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VHM69_09430"
     CDS             complement(<1..1271)
                     /locus_tag="VHM69_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2740657.1"
                     /translation="MSIILIVLFVVVAMVFAASLVLGRDTREVESARDTEYLELEGVW
                     IRYNVIGGGPAVLLVHGWLSSSRVWEQLAGRLAQRFTVYTLDLCGFGESDKPLSGYGV
                     RNGSRLLYAFCAHFGLTRANVIGHDLGGAMAVKLAADHPDVVGRLVIVSAPADEDQID
                     VPTMLWLATLPVIGPIFYALGRVARPVRRAWMRPFVADADDLTDEVVDDAGRSTPAAV
                     SKTLSISRREISRGRLARQARIIKVPLLAVAGEEDQIVDPQSVGMWASGVDKAEICLI
                     DECGHLPMIERTAEFNANILAFLTGDARYLDYAHPASRKDEDAAGYEEEEAETHFLEE
                     GEEYPTTTDSSDDTVDLTPRGMQPETRGENVPNVVRKHEGRYPARTARHPEASADGVE
                     ERSGSRARTRREPPDEDFLPELPDDLFDWPET"
     gene            complement(1540..2775)
                     /locus_tag="VHM69_09435"
     CDS             complement(1540..2775)
                     /locus_tag="VHM69_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740658.1"
                     /translation="MSSRAASWLAWTLVVLSLALLLGGIALSRVASSTVPNLRFGAES
                     ADGSVVADLVTLLTFSIVGAIIASRHPRNAIGWLFCSVGVTIGLNSFAGDYTEFWLAT
                     GFGTRSLGETAAWFASWLWILLIYVPTSFVLLLFPDGRLPSPRWRPVAWAAALGTAGG
                     VVGFALEAGPLGDFPQIVNPYGVDSSVVGIVGVAGGIVAAGAMVASAVSLIIRLRRAT
                     GVERQQLKWLAYGGTVMVGTIGVGGLIILWSVPVSIVIMSVSLLGLPLFTGIAIVRYR
                     LYDIDLLINRTLVYGSLTTMLVAVYVGGVVVLQYAFRVLSGGESQLAIVASTLAIAAA
                     FNPLRRRVQAFVDRRFYRRKYDAAKTLASFSARLRDETDLDTLSDELVGVVMETMQPG
                     HVSLWLRPDTATREIRNRA"
     gene            complement(2772..3506)
                     /locus_tag="VHM69_09440"
     CDS             complement(2772..3506)
                     /locus_tag="VHM69_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740659.1"
                     /translation="MAGMREMIGITAGRSNFKMATSFAYAAGATGILANVFLIAFFAL
                     QASHPEVGTSPGSANDLLVSLGTAFMIPDTISTLLWFERVGYALNLATTPAIPIAIGI
                     AILRYRLYDIDILINQTLVYGVPSATLVALYFGGVTLLQSLLGSLTGRESQLAVVAST
                     LVIAALFNPLRRRIQSFIDRRFFRRKYDARKTLEGFSASLRDETDLDSLTDELLSVVR
                     NTMQPAHVSFWLRSPTEGSRQMESRG"
     gene            complement(3748..3969)
                     /locus_tag="VHM69_09445"
     CDS             complement(3748..3969)
                     /locus_tag="VHM69_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740660.1"
                     /translation="MNDEMQKRVISILSTGIAYVLASQLADRLIQEPEVRGVRDDVKE
                     AVLHAGFSLASTIIASFVIRQVVSKRWGA"
     gene            complement(3988..>4269)
                     /locus_tag="VHM69_09450"
     CDS             complement(3988..>4269)
                     /locus_tag="VHM69_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740661.1"
                     /translation="LIVRHTNRRLGKLDRCEACERLISGRISFSEFVDQVGEEYRELL
                     DFMMENDLHPGRPEEVLFCIDCHAGLYERTLLRSQLEGLYSPDGSETVR"
BASE COUNT          863 a         1328 c         1314 g          764 t
ORIGIN      
        1 gtctcgggcc agtcgaagag gtcatccggc agttcaggca gaaagtcctc atcaggtggc
       61 tcgcgtcttg tccgcgctcg tgatcccgag cgctcttcga cgccgtccgc ggacgcctcg
      121 gggtgccgcg cggttctggc cgggtacctt ccctcgtgtt tgcggacgac attgggcacg
      181 ttctcgcctc gggtctcggg ctgcatgccg cgtggggtga gatccaccgt atcgtccgag
      241 gagtctgtcg tggttgggta ctcctcgcct tcttccagga aatgagtctc ggcttcttcc
      301 tcctcgtatc cggcggcatc ttcgtccttc cgcgaagcag ggtgggcgta atcaaggtag
      361 cgggcatcgc ccgtcaggaa ggcaaggatg tttgcgttga actcagcggt acgctcgatc
      421 atgggcaggt ggccacattc gtcgatgaga cagatctcgg ccttgtccac gccgctggcc
      481 cacataccga ccgactgggg gtcgacgatc tggtcctcct ccccggcgac ggcgagcagg
      541 gggaccttga tgattcgggc ctgcctggca agccgcccgc gcgagatctc gcgcctcgag
      601 atactcaggg tcttggagac ggcggcgggc gttgatctgc ccgcatcatc gaccacttcg
      661 tctgtgagat cgtcggcatc cgcgacgaag ggccgcatcc aggcgcggcg gaccggacgc
      721 gcgacgcgcc ccagagcgta gaagatgggc ccgatgacgg gcagggtggc gagccacagc
      781 atggtcggaa cgtctatctg gtcctcatcg gccggagccg agacgatgac gaggcgccct
      841 acgacgtccg ggtgatccgc cgcgagcttg accgccatgg ccccgccgag gtcgtggccg
      901 atgacgttgg cgcgggtcag gccgaagtgg gcgcagaaag cgtaaaggag cctgctcccg
      961 ttgcgtaccc cgtatcccga gagcggtttg tccgactctc cgaacccgca caggtcgagg
     1021 gtatacaccg taaagcgttg cgccagtcgc ccggcgagct gttcccagac gcgcgacgag
     1081 gatagccagc cgtgtaccag gagaaccgca ggacccccgc cgatgacgtt gtagcggatc
     1141 caaaccccct ccagctccag gtactccgtg tcgcgggcgc tctccacctc cctggtgtcg
     1201 cgtccgagta ccagggaggc ggcgaagacc atcgctacga ccacgaacag gactatcaat
     1261 atgatgctca tatccgccat gatagcgcaa aaacgggctg acaggatacc gggacagcgc
     1321 gagaaagcgc acctttcgcg ccggtgattg gcgtggtcag acaggcacct ctatcccgtt
     1381 tacggaccgc gtatcggggc ggcaaagcca gggtagcgag ttcgctaccg gggagatctc
     1441 tgctgcgcat cgacgcgtga atcccttaac catagaggga tccagcccgg cagcgatctc
     1501 cacatagtca ggtcatccgg cgcgatgccg ccccggatct catgcacggt ttcgtatttc
     1561 tctagtggcc gtatcgggac gcagccacaa agagacgtgc cccggttgca tcgtctccat
     1621 caccacccct accagctcgt cgctaagggt gtccagatcc gtctcgtcac gtagccgggc
     1681 gctgaaggaa gccagggtct tggcggcgtc gtacttcctg cggtagaaac ggcggtccac
     1741 gaacgcctgc acccggcgcc tcagcgggtt gaacgccgcc gcgatagcca gggtggatgc
     1801 cactatcgca agctgcgact cacctccact cagcacccgg aaggcgtact gcaagacgac
     1861 cacgccaccg acgtagaccg caacgagcat cgtggtcagc gagccgtata cgagggtgcg
     1921 gtttatcagg aggtctatgt catagaggcg atacctcacg atcgcaatgc ccgtaaagag
     1981 cggcaacccg agcaaggaga cggacatgat cacgatactg accggcacgc tccagagaat
     2041 gatcaagcca cccacaccga tggtaccgac catcaccgtg cccccgtaag cgagccactt
     2101 gagttgctgg cgctctactc ccgtggcacg gcggagccga attatgagcg agacggcgga
     2161 tgccaccatg gcacctgcgg ccacgatgcc acctgcgact cccacaattc ccacaacgga
     2221 gctatcgaca ccgtaggggt tcacgatctg tgggaagtct ccgagcggac cggcttccag
     2281 cgcgaagccc accacgccgc cggcggtccc aagcgcggca gcccaggcca cgggtcgcca
     2341 gcggggcgag ggcagtcgac cgtcagggaa caacagcaac acgaagctcg tggggacgta
     2401 aatcaggaga atccacaacc acgaagcgaa ccacgccgcc gtctcgccga ggctccttgt
     2461 gccgaaaccg gtcgcgagcc aaaactcggt atagtcccct gcaaaggagt tgaggccaat
     2521 cgtcacgcct acgctgcaga agagccaacc tatggcgttg cgggggtggc gtgaagcgat
     2581 gatggcgcca acgatcgaga aggtgagcag agtaaccagg tcagcgacca cgctgccatc
     2641 cgctgactcg gcaccgaatc ggagattcgg aacggtggag ctagccacgc gagacagcgc
     2701 gatcccaccc agaagcagtg ccagggatag caccaccagc gtccaggcca gccaggaggc
     2761 ggcgcggctg ctcatcctcg tgactccatt tgccgactcc cttcggttgg cgaccgcagc
     2821 cagaatgaga cgtgcgcagg ctgcatcgtg tttctgacca cacttaacag ctcgtcggtg
     2881 agcgagtcga ggtcagtttc atccctgaga cttgccgaga acccttcgag cgtcttcctc
     2941 gcatcgtact tcctgcggaa gaaacgacgg tcgatgaacg actggatgcg cctccgcagt
     3001 gggttgaaca gggcggctat tacaagggtt gaggctacaa ccgctagttg agactcgcgt
     3061 cccgtcaggc tgcctagcaa gctctgaagc aatgtcacac ccccgaagta caatgcgaca
     3121 agcgtcgcag agggcacgcc gtatacgagg gtctggttta tgaggatatc tatatcgtag
     3181 agtctgtagc gaagtatggc gattccgatg gcgatgggaa tagctggtgt cgtggccaag
     3241 ttcaatgcgt accctacccg ctcgaaccag aggagtgtgc ttatcgtatc gggtatcatg
     3301 aacgcggtcc caagcgacac gagaaggtcg ttggccgagc cgggcgaggt cccaacctca
     3361 gggtggctgg cctgcagggc aaagaaggcg atgagaaaca cgttggcgag gatgcccgtg
     3421 gccccggccg cgtaggcgaa actggtcgcc atcttgaagt tcgacctgcc tgccgtaatg
     3481 ccgatcatct ctctcattcc tgccatgagc gcatctttac gtcggtacca taaccggcac
     3541 acgtctcgta accgttacga tgagcgttac gagccagatc ttctgggaac gttcctggca
     3601 aacgtcaaac atgcgctatg agctgtcggc acgccaggat agggcgttgg atcgcctacg
     3661 aacctgagtt atgcctgcct ggccaggtcc cgtgcagatg cgtccgtgtt attcgtataa
     3721 gacccgccgc cgaagatccg ttcagcgtca ggcgccccag cgtttgctaa cgacctgccg
     3781 gataacgaag gaggcgatga tcgtcgaagc cagggagaat cctgcatgga gcacggcttc
     3841 ctttacgtcg tcgcggacgc cgcggacctc cggttcctga ataagcctgt ctgccagctg
     3901 gctagccagc acgtacgcga tccccgtcga gagaatcgag atcacacgct tctgcatctc
     3961 gtcgttcata gtgcgccccg ttctctgtca gcggaccgtt tcgctcccgt cagggctgta
     4021 gaggccttcg agttgggagc gaagtagggt gcgctcgtag agccctgcgt ggcagtcaat
     4081 acagaacagc acctcctcgg gacgtccggg gtgcagatcg ttctccatca tgaaatcgag
     4141 tagctccctg tactcttcgc cgacctgatc cacgaactcc gagaacgaga tgcggccaga
     4201 gatcaaacgc tcgcacgctt cgcagcggtc gagcttcccc agacgacggt tggtgtgcct
     4261 tacgatgag
//
LOCUS       DATAIR010000385         7145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_301892, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000385 DATAIR010000000
VERSION     DATAIR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301892"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..265
                     /locus_tag="VHM69_09455"
     CDS             113..265
                     /locus_tag="VHM69_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740662.1"
                     /translation="MELVAIFRLQGLGGRSWSAINKRLKVALRQCDALGIAVGAALAF
                     LVLNYV"
     gene            1541..2041
                     /locus_tag="VHM69_09460"
     CDS             1541..2041
                     /locus_tag="VHM69_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740663.1"
                     /translation="MDKIKRTSRTAERLAKTTGDSYKTVIDHVVAHQERNVRFAQELF
                     DGTAREVRHQAESNRALTQELVERAEVQREAFRTLVIESVDTYTDLLYAPLAYYRQGL
                     RLVESEVNGFPIAGYDEKNVKEISDRLDALTAAEIRTVREYEKRHKNRETLIEQFDRK
                     LRAVSA"
     gene            complement(2180..2368)
                     /locus_tag="VHM69_09465"
     CDS             complement(2180..2368)
                     /locus_tag="VHM69_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2740664.1"
                     /translation="MGRRDTTGKQDRNEGAMDKAKGRVKEAAGALTDDRDRKAEGRAD
                     QRRGTAKEKKGHFKDLLK"
     gene            2511..3053
                     /locus_tag="VHM69_09470"
     CDS             2511..3053
                     /locus_tag="VHM69_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2740665.1"
                     /translation="MKASTITRVALMAAVTAVAAQITVPISPVPFTLQVLAVILSGLL
                     LGVRTGALAQAIYVLVGAVGVPVFAGFKGGLGIVLGPTGGYLISYPIAAAVAGLAAHT
                     VARASRRKALWTGFLWGCAGLAVIYTLGATWLAVVAGLSPAEALATGVLPFVVFDLIK
                     VALAALVAVAAAPAIAPTRT"
     gene            3185..3736
                     /locus_tag="VHM69_09475"
     CDS             3185..3736
                     /locus_tag="VHM69_09475"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX2740666.1"
                     /translation="MQQVTRRRFTVHDYHRMGEAGILHEDDRVELIEGEIVEMAAIGT
                     RHFTCVNGLTRLLVRSVGDEAIVSVQNPVRLNQRTEPQPDLAVIRPRDYRVSLPGPED
                     VLLLIEVSDTTLAYDRNVKLPLYSRAGIREVWIVDLSGEVIERHNDPSPDGYRSLHRA
                     RRGEKIESSALPDPALTADDVLG"
     gene            complement(3762..5102)
                     /gene="gabT"
                     /locus_tag="VHM69_09480"
     CDS             complement(3762..5102)
                     /gene="gabT"
                     /locus_tag="VHM69_09480"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX2740667.1"
                     /translation="MLESTRIKTEIPGPKSRELMARRHKAVSSGLGIATPMFAKEAKG
                     ALLTDVDGNTFIDFGGGIGVLNVGHAHPRVVEAVKEQAERFLHTCFYVTEYEPYVELA
                     EKLNALVPGDTEKRSFFINSGAEAVENAVKIARSYTGRPSVIAFENAFHGRTLLAMTL
                     TSKTDPYKKHFAPFAPEVYRVPAPYPYRCPASKDCSGGCQGDCFNAVEKAFVSYVHPE
                     SVAAIIVEPVSGEGGFIPFPDFYLRKLRDLCDEHGIVMIVDEVQTGFGRTGKMFAVEH
                     SGVVPDLLITAKSLGGGMPIGGVTGKAEIMDAPHVGGLGTTYGGNPLACAAALAVIET
                     FEQEDLLGRANVVGERIMAAMRDIQEKHPDFVGDVRGRGPMAAMELVEDAASRTPDKV
                     RTGKVVEAALQEGLLLLTAGQYSNVIRTLAPLSIADDELEEGLAILARVFDQVA"
     gene            complement(5158..6609)
                     /locus_tag="VHM69_09485"
     CDS             complement(5158..6609)
                     /locus_tag="VHM69_09485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2740668.1"
                     /translation="MIETSMIESLFVGGEWSDSAASGKTFEVTNPANGERLATLPDAG
                     YEEMQSAIDAAAAAQGEWAATTAQYRAGIMREAARLMHERKEHLARVMTLEQGKPITE
                     SRGEIVYASSFVEWFAEEGKRVYGDVVPASFPDKRILVIKRPIGVTAAITPWNFPAAM
                     ITRKLGPALAAGCTMVIKPSELTPLSALELARIFEEAGLPQGVLSVVAGMDPAAITSA
                     IMDDRRVRKLSFTGSTEVGKLLMRQSADTMKRLSLELGGHAPFIVFEDADIDAAIEGA
                     MISKMRNMGQTCVCANRIFVQRPVVDEFSKKLTDEMAAMKVGDGLDEDVTVGPLIEPK
                     AIDKVEHHVADAKEKGARVILGGERTNGSGNFYPPTVLINADDTMLVAKEETFGPVAA
                     IMPFDTEDEVIERANDTPYGLAAYYFTRDVGRVMRLAENLEYGIIGANDGLPSTAQAP
                     FGGLKESGFGREGGHQGIEEYLDIKYVSLGGIV"
     gene            complement(6624..>7145)
                     /locus_tag="VHM69_09490"
     CDS             complement(6624..>7145)
                     /locus_tag="VHM69_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX2740669.1"
                     /translation="VLPAGLLGLVLAAALAAVMSTSSAGLLASSTLLANDVYARFVTH
                     RDHAGVGLDRLFTLIVGVVVLVISVLVSDVIGALTVAYDLLVGAIFVPVIGALFWRDA
                     TSAGALASMLAGSITVLVLLAWQGLLANSPIYVGLLVSLIVFVVVSLLTPRTSRETMS
                     AWDRRVSGRAQRS"
BASE COUNT         1294 a         2257 c         2169 g         1425 t
ORIGIN      
        1 taccttgtcg gacgatatat taaggaatac tatccgaatt tcaaactctt ggtgaccctc
       61 cgatgtcgat tgcgccatcc acgtgcactc tcacgttgat acgcagcatc cgttggagtt
      121 ggttgcgatc ttcaggctcc agggactcgg agggaggagc tggagcgcca tcaacaagcg
      181 cctgaaagta gctttgaggc aatgtgacgc cctcggcatc gccgtgggag ccgcccttgc
      241 atttcttgtc ctgaattacg tttaggaagg acgcagccgc ggctccgtag agcggccttc
      301 gccttgcccg gcccctaata atccccgccg gcggtccttg gccgtccggg gctatgagct
      361 atgcaccaga cctcagggtt aacatgattc tccgaacttg agtcacctcg tccctggtga
      421 ttcaccgtcg attcattgcc gcccgctatt gttgggtcgt tgaaaacgct cttggcaagg
      481 gagtaaaggg atcttgagat ctcagagtga tcggcgggaa agcgaaccct ctggaggggt
      541 gtccttcagc ttcagctaca aggagctcat agttacagga ctggccggag ccttgctcgc
      601 gttcctggct tcgaggtggc acatccccgg agtgttgcta ggggcactct cccccatggc
      661 atcgctcgtg atcatggcca tcgtcaaagc gtacttgagc ggagctgcga tcgatggtcc
      721 gagattgcca gggctcctat acatatttgg ggccttctgg tggttttcct ctcagtcagc
      781 aggaatgcgt cagtcgatac tgctggccgg ccttcgtgct ggggccgcgg ctaccgttat
      841 cagcgcgtcc gctgtagccg gcaccgaggt aatagcgggc gacgacttga cctgtcttat
      901 atgggatgag tgtcgaccgg cgattccccc tgatccgcca ccagacgagt ctacccaagc
      961 ggtggtagcg gctcccaatg ctttcgctgc ggctccaaat accgttgtcg aggacggtgg
     1021 gagcgtcgcg ctagtggccg cctctttgct aatcggctct ggtgtcttga ccgcggccat
     1081 tgtgcgtcgg cacggttagc gcagagttcg tctaaacgtc tcaagctgga tcacccagat
     1141 tgtttgcgcc ccctgccgga aattggtctc ctcatgtgag aagccgatag tacgagaggc
     1201 atccaacgtc aggccagtta gtgtaagatg tgtctatggg catagttgag ataatcgtga
     1261 ttctcgccgt cttggtgctc gtggtgctcg ccgtccggtg gttggtcctc cgcttacgcg
     1321 gatagaaccg tagagagacc cgctccaagt aactggcgat aagttccttc gtgccgctcg
     1381 ctcctccttt acgggccgcc acagacctgc gctcagtcga gaccttttgt gcaggttcct
     1441 ttagatttgg gagctatgta caaggccatc aacttccttg acatggttga acatatgtgt
     1501 atactgcaca tacgtaaagg cagctaacag gaggaaagac gtggataaaa taaagcggac
     1561 tagcaggact gcggagcggc tggcgaagac gaccggcgac tcctacaaga cggtcataga
     1621 ccacgtcgta gctcatcagg agcgcaacgt gaggtttgcg caggagctat tcgacggcac
     1681 ggcgcgcgag gtcaggcatc aggccgagag caaccgggcg ctgacccagg agctcgtcga
     1741 gcgggccgag gtacagcgcg aggcgttcag gacgctggtt atcgagtccg ttgacactta
     1801 cacggacctg ctgtacgcgc cgctggcgta ctacaggcag ggtctgcgcc tcgtcgagag
     1861 cgaggttaac gggttcccga tcgccggtta cgacgagaag aacgtcaagg agatcagcga
     1921 ccgcctcgac gccctcaccg cggcggagat ccgcacggtg cgcgagtacg agaagcggca
     1981 caagaaccgc gagaccctga tcgagcagtt cgaccgcaag ctcagggccg tctctgcgta
     2041 agtaggcaac gggtttcagc cgtcaggtag tacgtcagcc tagactggca ccgccaaacg
     2101 cggctggtaa cggaaagggc cggggaataa ttccccggcc ctttcgcctt tcgatttact
     2161 tctaactgat acctgatgcc tacttcagca agtccttaaa gtgtcccttc ttctccttcg
     2221 cggttccgcg ccgctggtca gcgcggcctt cggctttcct gtccctatcg tcggtaaggg
     2281 cgcctgcggc ctccttgacg cggcccttgg ccttgtccat cgcgccttcg tttctatcct
     2341 gcttacctgt cgtgtctcgc ctacccacct gttcctcctc atcccggaaa cgttagttac
     2401 atttgtttac cctgaggcac agggatcaaa cgtcagggtg caggaaacgc ggccccaacg
     2461 gtggtaaaag aggttgggtg tgacaatccc gataatcaga aaggccggcg ttgaaggcat
     2521 caaccataac ccgtgtggcg ttgatggcgg cggtgaccgc ggttgcggcc cagatcaccg
     2581 ttccgatctc tcccgtcccg ttcaccctgc aggtacttgc cgtcattctt tccgggctcc
     2641 tgctcggggt gcgcacaggt gcgctcgccc aggcgatcta cgtcctcgtc ggggcggtgg
     2701 gtgtgccggt cttcgccgga ttcaagggcg gactcggcat tgtactcggg cctacgggag
     2761 ggtatttgat ctcctaccct atcgccgccg cggtcgccgg cctcgcggcc cacaccgtcg
     2821 cgcgagcctc gcgccgcaag gcgctgtgga caggctttct ctggggatgc gcgggcctcg
     2881 cggtcatcta caccctcggg gctacctggc tggccgtggt tgccggcctc tcccccgccg
     2941 aagccctcgc gacaggcgtc ctgcctttcg tggtcttcga cctcataaaa gtagcgctgg
     3001 ccgcgctcgt cgccgtcgcc gccgcgcccg ctatcgcccc cacgcgcacc tgaaggacaa
     3061 aatcgaagcc agtatgaagc ttcgcggatt acctggagaa agcctaaagg tatcgtctca
     3121 tggtccgagt atacttctgc atgggacgag cgaaccatcg gaggttacgt cgttggagtc
     3181 gagcatgcaa caggttaccc gccggcgctt caccgttcac gactaccacc gcatgggcga
     3241 ggccgggatc ctgcacgaag acgaccgggt cgagctcatc gagggcgaga tagtcgagat
     3301 ggccgccata ggcaccaggc acttcacttg cgtcaacggg ctgacccggc tgctggttag
     3361 aagtgttggc gacgaggcca tcgtcagcgt ccagaaccct gtaaggctca accagcgcac
     3421 cgagccccag ccggatctcg ccgtgatcag gccccgcgac taccgggtct cgctccccgg
     3481 tcctgaagac gtgctgctcc tgatcgaagt ctcggacact accctggcct acgaccgcaa
     3541 cgtcaagctg ccgctgtatt ccagggcggg cataagggag gtctggatcg tggacctctc
     3601 cggcgaggtc atagagcgcc acaacgaccc ttcaccggat ggataccgta gcctccacag
     3661 agcgcggcgt ggggagaaga tcgagtcttc cgctttaccc gatccggccc tgaccgcgga
     3721 cgacgtgctc ggctgaccct cgtcccgtac cggtcagttg atcatgccac ctgatcgaag
     3781 accctggcaa gaatagccag tccctcctcc agctcgtcgt cggcgatgga gagcggtgcg
     3841 agggttcgga taacgttgct gtactggccc gcggtcagga gcagcagacc ctcctggaga
     3901 gcggcctcga cgaccttgcc ggtacgcacc ttgtcggggg ttcgactcgc ggcgtcctcg
     3961 acgagctcca tcgccgccat cgggccgcgc ccgcgcacgt ctccgacgaa gtcagggtgc
     4021 ttctcctgga tgtcacgcat ggcggccatg atgcgctcgc caacgacgtt ggccctgcct
     4081 agtagatctt cctgctcgaa ggtctcgatg acggcgagag cggcggcgca ggccagaggg
     4141 ttgccgccgt aggtcgtgcc caggccgccg acgtgtgggg cgtccatgat ctcggccttc
     4201 cccgtaacgc cgccgatggg catcccgccg ccaaggctct tggcggtgat caggaggtca
     4261 ggcacgacgc ctgagtgctc gacggcgaac atcttgcccg tacgcccgaa gccggtctgt
     4321 acctcgtcga cgatcatcac gatgccgtgc tcgtcgcaca gatccctgag cttgcgcagg
     4381 tagaaatcag ggaacgggat gaaaccgccc tcaccggaga cgggctcgac gatgatcgcc
     4441 gccacgctct cgggatgtac gtagctgacg aaagccttct cgacggcgtt gaaacagtcg
     4501 ccctggcagc ctcccgaaca atccttgctc gccggacagc ggtaaggata cggagccggc
     4561 acccggtaga cctctggggc gaagggcgcg aagtgtttct tgtaagggtc ggtcttgctc
     4621 gtcagcgtca tggcaagaag agtgcgtccg tggaaggcgt tctcgaaggc gattacggag
     4681 gggcgccctg tatacgagcg ggcgatcttg accgcgttct cgacggcctc ggcccccgag
     4741 ttgatgaaga aggaacgctt ctcggtatcg ccaggtacca gcgcgttgag cttctcggcc
     4801 agctctacgt aaggctcgta ctcggtgacg tagaaacagg tgtgcaggaa gcgctcggcc
     4861 tgctccttga ccgcctcgac gacccgcggg tgcgcgtgcc cgacgttcaa aaccccgatg
     4921 ccgccaccga agtctatgaa cgtgttgccg tcgacgtccg tcaggagcgc gcccttcgcc
     4981 tccttggcga acatcggcgt cgcgatcccc aaccccgacg atacagcctt gtgccgccgc
     5041 gccatcaact cccggctctt cggacccggt atctcggtct tgatcctcgt tgactcgagc
     5101 atcgtcgcca cctcttcctg tcggcctaac tcattccagt atagctttcg tccgctacta
     5161 gacgataccc ccgagcgaga cgtacttgat gtccaggtac tcctcgatgc cctggtggcc
     5221 tccctcgcgt ccgaagcctg actccttcag gcccccgaaa ggcgcctggg ccgtcgatgg
     5281 cagcccgtcg ttagccccta tgatgccgta ctccaggttc tctgccaggc gcatcacacg
     5341 ccccacgtcg cgggtgaagt agtaggccgc gagcccgtaa ggggtgtcgt tggcgcgctc
     5401 tatgacctcg tcctccgtat cgaagggcat gatggccgcc acggggccga acgtctcttc
     5461 cttggccact agcatggtgt cgtccgcgtt aatcagcacc gtcggaggat agaagtttcc
     5521 actaccgttc gttcgctcgc cgccgaggat caccctcgcg cccttctcct tcgcgtccgc
     5581 gacgtggtgc tcgaccttgt ctatggcttt aggttcgatc aggggcccga ccgtaacgtc
     5641 ctcatcaagg ccatcgccga ccttcatggc cgccatctca tctgtcagct tcttcgagaa
     5701 ctcgtccacc acgggccgct ggacgaagat gcggttggcg cacacgcagg tctggcccat
     5761 attgcgcatc ttcgagatca tggctccctc gatcgccgcg tcgatgtcgg cgtcctcgaa
     5821 gacgatgaag ggagcgtggc cgccgagttc caggctgagc cgcttcatcg tgtcagcgga
     5881 ctggcgcatc aatagcttgc ctacctccgt cgagcccgta aaagagagct tgcggacgcg
     5941 cctgtcatcc atgatggccg aggttatggc ggcagggtcc atcccggcga ccacggagag
     6001 gacgccctgc ggaagaccgg cttcttcgaa gatacgggcc agttccaggg ccgagagggg
     6061 cgtcagttcc gagggtttga tcaccatcgt gcagcctgcg gccagagcgg ggccgagctt
     6121 gcgtgtgatc atggcggcgg ggaagttcca cggtgtgatc gccgccgtta ccccgatggg
     6181 gcgctttatg acaaggatgc gcttgtcggg gaaactagcg gggaccacgt cgccgtatac
     6241 gcgcttccct tcttcggcga accactcgac gaacgaagag gcgtagacga tctcaccgcg
     6301 ggactcggtt atgggcttgc cctgctccag cgtcatgacc cttgcgaggt gctctttgcg
     6361 ctcgtgcatc agcctggcgg cctcgcgcat gatcccggcg cgatactgag ccgtagtcgc
     6421 cgcccactcc ccctgggccg cggcagcggc gtcgatggcg ctctgcatct cctcgtatcc
     6481 ggcgtcaggc agggtggcga gcctctcgcc gtttgccggg ttcgtcacct cgaacgtctt
     6541 gcctgacgcg gctgaatccg accactcgcc accgacgaac aggctctcta tcatgctggt
     6601 ttcgatcact tttcctccag agttcaggat ctctgcgctc gcccgctcac cctgcggtcc
     6661 caggccgaca tcgtctcccg agaagtccgt ggcgtgagca ggctcaccac tacgaacact
     6721 atcaggctga caaggagtcc gacgtagatc gggctgttcg cgagcaagcc ctgccaggcc
     6781 aacagcacca agaccgtaat gctgccggcg agcatggatg ccagggcgcc ggcgctcgtg
     6841 gcatcgcgcc agaacagcgc accgatcaca ggcacgaaga tggcgccgac gagcaggtcg
     6901 taggcgaccg tgagcgcccc gatcacatcg ctcaccagta cggagatcac cagcaccaca
     6961 acgccgacga tgagtgtaaa aaggcggtca aggccgaccc cggcatggtc tctatgcgtc
     7021 acgaaacgcg cgtagacgtc gttcgcaaga agcgtcgatg aggccagcag ccccgcgctc
     7081 gacgtggaca tgacggcggc gagggcggct gcaagcacca ggccgaggag accggcgggg
     7141 agtac
//
LOCUS       DATAIR010000386         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_394005, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000386 DATAIR010000000
VERSION     DATAIR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394005"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1586
                     /locus_tag="VHM69_09495"
     CDS             141..1586
                     /locus_tag="VHM69_09495"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX2740670.1"
                     /translation="MSPAGDHVPGGAVDAAEYLASQPPELRVPDALEKIRHEVGESGR
                     RLVVLDDDPTGTQTVHGVPVLTTWSADDLRWALEQPSSTFYVLTNSRSFPEDQAAGMN
                     REISTNLASAAGQTGTGFAIVSRGDSTLRGHYPAETDALEEGLGVDFDGVVLCPCYLE
                     AGRLTVDDIHWVRQGERLVPAGETEFARDASFGYSSSNLAEWVEEKSNGRVPASEVAS
                     VGLSDIREGGPERVAEVLLGVSGGKPVVVNAASYADLEVFVLGLLDAEEKGKRFLYRT
                     GPSLVRVRGGISEKGPLGAEELYGSRPRRGHGLVLVGSHVEMTTRQLEQALALDGVRS
                     VELSVPRLLDAGERDGELDRVVSEVNRGLIEAEVVVYTSREVVHGGATGFEIGVSVSD
                     ALVEVMRRVDRGLPLAFVVAKGGITSSDVGTRGLGVRRAEVAGQMLPGIISVWILPDD
                     SAFPGLPYVIFAGNVGEEDSLARVIEILREG"
BASE COUNT          276 a          481 c          562 g          267 t
ORIGIN      
        1 taagtgcgag ccccatcacg gtcgaggttg ccctgaaggc cgcggcgact actcctggaa
       61 ccaagagcaa ggatgcacgt tcgatcacgc ttctcaacgc cggtagcttt taacggacca
      121 gggacggaac cggagagacc atgagccccg ctggagacca cgttcccggc ggggctgtgg
      181 acgccgcgga gtacctcgcc tcgcagccgc ccgagctcag ggtccctgac gcactggaga
      241 agatccggca cgaagtcggc gagagcgggc ggcgcctcgt cgtccttgac gacgatccga
      301 cgggcaccca gaccgtccat ggcgtccctg tgctgacgac gtggtccgca gacgacctgc
      361 ggtgggcgct agaacagcca tcatccacgt tctacgtgct taccaactcg cgcagcttcc
      421 ccgaggatca agccgccggg atgaatcgtg agatctcgac gaacctcgct tcggcggcgg
      481 ggcagaccgg caccgggttc gccatcgtca gccgaggtga ctctaccctg cgcggccact
      541 acccggcgga gacggacgcg ctggaggaag gactcggtgt ggacttcgac ggcgtcgtcc
      601 tctgcccctg ttacctggag gccggacgtc tcaccgtgga cgacatccac tgggtgcgcc
      661 agggagagag gctcgtgcca gcgggagaga cggagttcgc ccgcgacgcc agcttcggct
      721 actcctcctc gaacctcgct gagtgggtcg aggagaagtc gaacggccgc gtcccggcct
      781 ccgaggtagc aagcgtggga ctctcggaca tcagggaggg agggccagag cgggtcgctg
      841 aagtccttct cggcgtgagc ggcgggaagc ccgtcgtggt gaacgcggcc tcgtatgccg
      901 acctcgaagt cttcgtgctc ggcctgctcg acgcggagga gaagggcaaa cgcttcctgt
      961 accgcacggg accttcttta gtcagggtgc gcggcggcat ctccgagaaa ggacctctcg
     1021 gcgcggagga attgtacggc agccgtccgc gtcgaggtca cgggcttgtg ctcgttggtt
     1081 ctcacgtgga gatgaccaca cgccagttgg aacaagctct cgcgctggat ggagtaaggt
     1141 ccgtcgagct ctcggtgccg cggttgctcg acgcagggga gcgggacggg gagttggaca
     1201 gggtggtttc cgaggtcaac aggggactca tcgaggccga ggtcgtcgtg tacacgagca
     1261 gggaggtagt gcacggcggc gcgacagggt tcgagatcgg ggtctcggtc tcggatgcac
     1321 tggttgaggt gatgcgccgg gtggacagag ggttgccgct cgcgttcgtc gtggcgaagg
     1381 gtggtatcac ctccagcgac gtcggaaccc gcggtctcgg cgtgcgaagg gccgaggtcg
     1441 ccggtcagat gcttcccggc ataatatccg tctggatcct gcccgacgac agcgcctttc
     1501 ccggcctgcc ctacgtcatc ttcgccggca acgtgggcga ggaggactcg cttgccaggg
     1561 tcatagagat cctcagggag ggatag
//
LOCUS       DATAIR010000387         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_302176, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000387 DATAIR010000000
VERSION     DATAIR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302176"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..1393
                     /locus_tag="VHM69_09500"
     CDS             635..1393
                     /locus_tag="VHM69_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740671.1"
                     /translation="MGAIDSWLEGFMQGQIGVGVILLISLLLGLRHASDPDHLAAVTT
                     LIASAEERDQVRKAGLMGFLWGLGHGTTLVLVGLPLVLLNLYLPEVVSRIAELAIGCI
                     IILLAVRLLVRWQRGLYHVHVHTHDGGETHRHVHSHAHDESHKHAHRVPRRTPLSSYG
                     VGLVHGIGGSGGLTLLLLSTISDKLQATGALLLFAAGTAVSMALLSTAFGLVIAGGPV
                     ARNFERVAPVLGVLSLAFGAWYALGALELVIYPF"
     gene            complement(1757..>2657)
                     /locus_tag="VHM69_09505"
     CDS             complement(1757..>2657)
                     /locus_tag="VHM69_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX2740672.1"
                     /translation="RGGFARIGLVDPDEEEFASVSRELDLHEMAVRDAIKSHQRPKLE
                     RYGEALFVVFKTAHYLDEEERVEFGEIHVFLGRDFIVTVRHGEASSLEGVQKTLEGEP
                     EFLRRGPESVLWAVMDRFVGDYEPVVEGLGNDLDEIEEEVFGGNAGASRRIYELSREV
                     LGFRRAAQPLTKVLEHLLGETYAFDSEVARRMRGVHDHVLRISEQIEGFLELLSNILN
                     VNLTLVSVEQNAAVQRQNVQIQKISAWAAIVAVPTLISGVYGMNFRYMPELHWLLGYP
                     FALLMMGVISGLLYRGFRRAGWL"
BASE COUNT          492 a          873 c          786 g          506 t
ORIGIN      
        1 atccgctcgt gagagcgact tgtaggcgtc catccacgcc cgccttgcgt aggactcacg
       61 gccccttccg agttcgtcga tggcatccac cacagctact ccaatcggtc tggcagagcg
      121 cctcggcccg ctgcgcgcac caagaatgag ccgggacggc agccacagtc ggcgtcgagc
      181 aacacacgca accccggcgc cctgctcgca aagttcgggg agtccccact ccccgcttcg
      241 tgaagacccc gggcggccgc ttgactcaat tttcgcatct ctatgtttcc gcacttccca
      301 gcagagtatt tgatctcaat gtagcaaaag catgaggatg gcgctacttc caagtgaacg
      361 cctcagagga aggtgtagtg cttcagcgct ccgggtggaa gtgttcctcg acgagcccta
      421 tgactgtgct cctctcgatg tccaggacgc ccgtgaagag aaggtactcg ttcactatcg
      481 gcatgttacc caaggccggc cacggtctgc tcacgggcgc gggctggtgg gtctgggata
      541 ctatggttat gtttctacgc gctggacggc ttcaggacgc gtatagtggg aaaccagccg
      601 tgtccggcgg cgagacggag gctaagagga gtgcgtgggc gcgatagata gctggctcga
      661 agggttcatg cagggacaga tcggcgtcgg cgtcatcctg ctcatcagcc tgctgcttgg
      721 cttgcggcac gctagcgatc ccgaccacct cgccgctgtc acgaccctga tcgcctccgc
      781 ggaggaacga gaccaggtac gcaaagccgg cctgatgggc tttctctggg gcctgggaca
      841 cgggaccacg ctcgtgctcg tcggcctgcc gctggtcctc ctgaacctgt acctgcccga
      901 ggtcgtgagc cggatcgccg agctcgccat cggctgcatc ataatattgc tcgcggtgag
      961 gcttctcgtc aggtggcagc gaggtctcta ccacgtccac gtccacaccc acgacggtgg
     1021 cgagacccac cgtcacgtcc actcccacgc acacgatgag tcccacaagc acgcccaccg
     1081 cgtcccacgg cgcactccac tttcgtctta cggcgtcggt ctggtgcacg gcatcggagg
     1141 ttcgggagga cttacgctcc tgctgctctc gaccatctcg gacaaactac aggcgaccgg
     1201 agcgctgctg ctcttcgcgg ccggcaccgc agtctccatg gcgctcctct cgacggcctt
     1261 cggcctcgtc atagcaggcg gtcccgtcgc gcgcaacttc gagcgcgtcg cccccgtgct
     1321 cggcgtcctt agcctcgcat tcggggcgtg gtatgcgctc ggggcgctgg aactcgtcat
     1381 ctatccgttc tagcaacgcc ggttgacagc aaacgcgtag aacgagtagc accgaacaca
     1441 agaccggcgc gtagacttca aatcgggttg atagtggaaa acgtaccgat actcgcttcg
     1501 gacgccagga ctcggctgcg cgcctcggct tcgagcacgg cgccgacctc gtcaaatcct
     1561 attacacgga ggacttccgc aaggtgacgg acaattgccc cgtccttgta gctggcggtc
     1621 ccaggatgga gaccgtcgaa gagatgttac aggccgtcgc agatgcgctc gccgaatcag
     1681 aggttgcatt acgtgaacga acgagctgac agcttatggc acgattcgct gaagtgacct
     1741 tcagcgaatc gccttactac agccagcctg cgcgcctgaa gccccggtag agcaggccgg
     1801 agattacgcc catcatcagt agggcgaacg ggtaaccgag gagccagtgc aattccggca
     1861 tgtagcggaa gttcatcccg tagacgcccg atatcaaggt gggcacggcc acgatggcgg
     1921 cccaggctga gatcttctgt atctgcacgt tctggcgttg caccgcggcg ttctgctcga
     1981 cgctgacgag ggtgaggttg acgttgagga tgttcgagag cagctcgagg aagccctcga
     2041 tctgctccga tatccgcagg acgtggtcgt gcactccgcg catcctgcgt gccacctcgc
     2101 tgtcgaaagc gtaggtctct ccaagcaggt gttccagaac tttggtcaat ggctgcgcag
     2161 cccggcggaa ccccagcacc tcccgcgaga gctcgtagat cctgcgcgag gcacccgcgt
     2221 tgcctccgaa gacctcctct tcgatctcgt cgaggtcgtt ccccagcccc tccacaacgg
     2281 gttcgtagtc gccaacgaac cggtccatca cggcccagag cactgactcg ggacccctgc
     2341 ggaggaattc gggctcgccc tccagcgttt tctggacgcc ttcgagcgac gatgcttcgc
     2401 cgtgcctgac ggtgactatg aaatcccgtc ccaggaaaac gtggatctcg ccgaactcca
     2461 cccgctcttc ttcgtccagg tagtgagccg ttttgaacac cacgaagagc gcttccccgt
     2521 aacgctccag cttgggacgc tggtgggact tgatcgcatc gcgcacggcc atctcgtgca
     2581 ggtccaactc gcgggacacg gaggcgaact cctcctcatc cggatcgacg agccctatcc
     2641 gggcaaaccc gccccgc
//
LOCUS       DATAIR010000388         5687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_394198, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000388 DATAIR010000000
VERSION     DATAIR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394198"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1723
                     /locus_tag="VHM69_09510"
     CDS             188..1723
                     /locus_tag="VHM69_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2740673.1"
                     /translation="MKRRVVPSCTVRRRTLTRGDFLKLTGAAMAGATLLGATSCDLTE
                     RIRNIKNDRPSLKEGTNVVLVIIDSLRKDHIGAYGNDTIHSPNLDALAKESLRFTQAY
                     PESIPTICARRAIHTGRRTWPFKDWKPPKGEDIILQGWEPIPNDQVTLAEMMRAAGYG
                     TYFVTDNMHQFKPSYNMHRGFDVFDFFRGQTTDNYKPIWTYPRDKVDQALLKGNVPAM
                     TGQMRQYFANVKERRTEADWFSPMVFSQASDYLEVLSKGGPFFLTVDSYDPHEPWDPP
                     EEYVTMYDDEPYNLKEPFSVIYGQSDYLEPRELQRMKARYSAELTMMDRWLGRFLDKM
                     EELNLFENTLLILLSDHGVAHGEHGYTGKPDNVLWPEVTDIPFLIRHPEGIGAGKTND
                     YYASTHDVAPTILSFLGVEPRQELDGADLSVVLDGGEAEARPHFSIGYNDHVWTRDDR
                     YVMFSTNQGEDPKLYDVQEDPGMHNNLAGSEPNIVKRMFEGYILRDAGGPLPTYDFDT
                     QGG"
     gene            complement(2124..2894)
                     /locus_tag="VHM69_09515"
     CDS             complement(2124..2894)
                     /locus_tag="VHM69_09515"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX2740674.1"
                     /translation="MAARSIDEVIEQLDEIIDLARRQKSRLGYFAALYRNVTSNVKAG
                     IFAGNFEDAERMERLDVAFANRYLDALERYRRGEEISKCWRTSFRAAQEWHPIILQHL
                     LLGVNAHINLDLGVAAAQTAPGTQLASLRRDFDGINDVLWVMLDEVQDKLRQVSPLMG
                     WLDLAGGSGDEVILNFSIKRARQAAWRVAERLAPLGPEAMHKEIDVLDGWVDVLAGVI
                     EHPPENTIGVARFFVRVSESRDVGKVIDVLAGDQHQVR"
     gene            3148..4116
                     /locus_tag="VHM69_09520"
     CDS             3148..4116
                     /locus_tag="VHM69_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2740675.1"
                     /translation="MQKVLSIDGGGIRGIIPAMVLAEIERRTEKRIAEMFDLVAGTST
                     GGILALGLTKPGQDGKPEYSAKKLIELYETEGGRIFSIPVWHRIKSAGGVLEEKYPSE
                     GIEEVAKEYFGDVRLAQAHKEVLVTAYEIEKRSPWFFKRRHALDPTKKGYNPFMREVA
                     RATSAAPTYFEPLQLPVGAEGDLAFIDGGVHSNNPAMCAYVEAMKINPDEKDFLVVSL
                     GTGELTRNMPYEEVKGWGLALWAQPILNVVFDGVADTVDYQLRELLSSEGDDRRYYRF
                     QTVLDIGKDDMDDASRTNIAALKVKAREIIAENDSALKALCAQLVA"
     gene            complement(4176..5666)
                     /locus_tag="VHM69_09525"
     CDS             complement(4176..5666)
                     /locus_tag="VHM69_09525"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX2740676.1"
                     /translation="MKGKITRRRFLGAATAGAAWVALGGTVGCEPETRAKATARANSS
                     PAQGDKTWAFHSRPDLKPPVIRVTTQAQDTAPGYVFCAPKSGPGESGPGQDGCLILDN
                     TGQPVWFRPAPGEAMDAMDFKVQIYKGETVLTWWEGHHTSYGQGEFVICDHAYREIKR
                     VRAGNVYRADHHEFMITPQDTALLDIYHKVTMDLSEHGGEKGATVLDGIVQEVDIESG
                     DVLFEWHSLEHVGLEESYVLPYDYFHLNSIDVDHDGNLLVSSRTTCTIYKIDRRSGEV
                     IWRLGGKRSDFEMGPGTRTNFQHDARRQKDGTLTVFDNGNMNEVDQSRAIVLELDEEA
                     MTARLVREYTHPDKVRSSTQGNVQVLPDGNVFVGWGSEPVLSEFSHDGRLRFSAAFPT
                     EGESYRAFRFPWSGQPQEDPALVAKRGPEDNVTLYASWNGATEVATWQVLAGPGPDRL
                     EVVASAPRKGFETSIVLRTTEPYVGVQARDLSGRVLGASKALKPGG"
BASE COUNT         1163 a         1755 c         1677 g         1092 t
ORIGIN      
        1 ccattccgaa aaagcgcgtg aagaccggcg cgaatatacc cggcaccagc agtagaccga
       61 gcactagcgc gagcaacact ccgatgccca cggccacaat gctcctcata agaaccattc
      121 tagcaggccc ctcaccgagt ccacgtagcc gccttcaccg acatacctct cccctgaggc
      181 ttgtagggtg aagcgccgcg tagtaccctc ttgcaccgtg agacggcgta cgctaacccg
      241 cggagatttc ctcaagctga ccggcgctgc gatggccggg gcgacgctgc tcggtgcgac
      301 gagctgcgac ctaaccgagc gcatacgcaa catcaagaat gatcgtccca gtctcaaaga
      361 gggcacaaac gtggtactcg tcatcatcga cagcctgagg aaggaccaca tcggcgccta
      421 cggcaacgat acgatccaca gccccaacct ggacgccctg gcgaaagaga gcctgcgctt
      481 cacccaggca taccctgagt ccataccgac gatctgcgcg cggagggcca tacacacggg
      541 cagacgcacc tggccattca aggactggaa gccacccaag ggcgaggaca taatcctgca
      601 gggctgggag ccaataccca acgaccaggt caccctcgcc gagatgatgc gcgcggcggg
      661 ttacggcacc tacttcgtaa cggacaacat gcaccagttc aagccctcgt acaacatgca
      721 ccgaggcttc gacgtcttcg acttcttccg cggccagact accgacaact acaagccgat
      781 ctggacttac ccgcgggaca aggtagacca ggccttgctc aagggcaacg tcccagccat
      841 gacgggccag atgcgccagt acttcgccaa cgtcaaggag aggaggacgg aggccgactg
      901 gttctccccc atggtctttt cccaggcgtc tgactacctc gaagttctta gcaagggtgg
      961 tcctttcttc ctcacagtgg actcatacga cccgcacgag ccctgggacc cgcccgagga
     1021 gtacgtcacg atgtacgacg acgagccata caacctcaag gagccgttct cggtgatcta
     1081 cggccaaagc gattaccttg agcctcggga gctacagagg atgaaggcgc gctactcggc
     1141 ggagctcacg atgatggacc gctggctcgg gcgcttcctc gacaagatgg aggagttgaa
     1201 cctcttcgag aacaccctcc tcatactcct ctccgaccac ggcgtcgccc acggcgaaca
     1261 tggctacaca ggcaagcccg acaacgtgct ctggccggag gtaaccgata tcccgttcct
     1321 aataagacac cccgaaggca tcggagccgg gaagacgaac gactactacg cctcgaccca
     1381 cgacgtggcg ccgacgatcc tcagcttcct cggcgtagag ccgcggcagg agctggacgg
     1441 cgcggacctc tccgtcgtcc tcgacggcgg cgaggccgag gcgagaccac acttcagcat
     1501 cggctacaac gaccacgtct ggaccaggga cgaccgttac gtcatgttca gcaccaacca
     1561 gggggaagac cccaagctct acgacgtgca ggaagacccg ggcatgcaca acaacctggc
     1621 cgggtcagag cccaacatcg tcaagcggat gttcgaaggc tacatactga gagacgccgg
     1681 cggtcccctg cccacctacg acttcgacac ccaaggcggc taacccgccg cccttcggca
     1741 aagcaccatc gactcgttag agacttgaac ccgatgctgt aactgtcgtc cgtatcgtcg
     1801 ccgcgtgcct cgatctctcg tacgctggag ccccatctct gacagcggct tgcggaagga
     1861 ttgatcctcc ccaagctagg tttcaggcat caggaaggtc ttcctggacg gacggtggtc
     1921 gctaaggtac acctctgggg cgaggtggat caatacttat ctactgcgtt gtgagatcca
     1981 gatgtgtttg agtgtaggat ggcggcgatt cgtcgccatc ctatctgctc tttccctgtc
     2041 aacgtgctgc acgacgaagt cacgtcagca ttcccagctt acgggctgtg ggagacgaac
     2101 ggaatgtgag taaagagcta tggttagcgc acctggtgct gatctccggc gaggacgtca
     2161 ataactttgc ccacgtcgcg gctttcactg acccggacga aaaaccttgc cactccgatc
     2221 gtgttctcag gagggtgttc gatgacccca gccaaaacgt caacccagcc gtcgagcaca
     2281 tctatctctt tgtgcatcgc ttctggtccc agcggcgcca gacgctccgc cacgcgccat
     2341 gccgcctggc gagcacgttt gatgctgaag ttcaagatca cttcgtcgcc gcttccgcct
     2401 gccaggtcaa gccagcccat caacggcgag acttgcctca gtttgtcctg aacttcatcg
     2461 agcatcaccc acagaacgtc attgataccg tcaaagtctc gcctcagact cgctaattgc
     2521 gtgcctggcg ctgtttgggc tgcggcgact cccagatcga ggttgatgtg agcgttgacg
     2581 ccgagcagca ggtgttgaag gattatggga tgccactcct gggctgctct aaaggaggtt
     2641 cgccagcact tgctgatctc ttcgccccga cggtaacgtt cgagggcatc gagataacga
     2701 ttggcaaacg ccacgtccag gcgttccatg cgctctgcgt cctcgaagtt gccggcgaag
     2761 atgcctgctt tcacgttgct cgtaacgttg cggtagaggg cggcgaagta gccgagtctg
     2821 ctcttctgac ggcgagcgag gtcgattatc tcatcaagtt gctcgatgac ctcatcgatc
     2881 gaacgggcgg ccataaaact ccctaatcac ttttggttga gtatagaaag tcgggcggac
     2941 tgccgccaca ggtgatcttc ccactcttcg aaagccaagg gtctccgcag gcgcggagac
     3001 atccatacgc gggttctgga acatacttgc aggccagttg agacgctttt ttggtctgaa
     3061 ggaaggaacg tgagcctcga atcattccgg attcatcgaa atctgctata gagggagtac
     3121 cagtacccta cgtaaggagg aaggcggatg cagaaggtac tttcgatcga cgggggtggc
     3181 atcaggggca tcataccggc catggtcctc gctgagatcg agaggcgtac ggagaagcgg
     3241 atcgcggaga tgttcgacct ggtagcgggt acctcgaccg gcggcatcct cgccctgggt
     3301 ctgaccaagc cgggacagga cggcaagccc gagtacagcg cgaagaagtt gatcgagctc
     3361 tacgaaaccg agggcgggag gatcttctct ataccggtgt ggcatcggat aaagagcgcg
     3421 ggcggcgtgt tggaggagaa atatccttcg gaaggcatcg aggaggtcgc gaaagagtac
     3481 ttcggcgatg tccgcctcgc gcaagcccac aaggaggtcc tggtcaccgc ctacgagata
     3541 gagaagcgca gcccctggtt cttcaagcgc aggcacgcac tggacccgac taagaaaggg
     3601 tacaacccct ttatgaggga ggtcgcgcgg gctacctcag ccgctcccac ctacttcgaa
     3661 cccctccagt tgccggtcgg ggccgagggg gacctcgcct ttatcgacgg cggggttcac
     3721 tccaacaacc ctgccatgtg cgcctacgtc gaggcgatga agataaaccc agatgagaaa
     3781 gacttcctgg tggtctcatt gggcacgggg gagttgacgc gcaacatgcc ctacgaggag
     3841 gtgaagggct gggggctcgc attgtgggcc cagccgatcc tgaacgtcgt cttcgacggt
     3901 gtggccgata cggtggacta ccagctgcga gagctcctca gctccgaagg agacgaccgg
     3961 cgctactacc gcttccagac cgtgctagac atcggcaagg acgacatgga cgatgccagt
     4021 cgcacgaaca tcgctgccct gaaggtaaag gccagggaga tcatcgccga aaacgacagc
     4081 gccttgaaag ccctgtgcgc ccaattggtc gcgtaacgga ggtttcgaac agagagcagc
     4141 tctcagtctt tggaatccgc caccgggagc gccggttaac ctcccggctt cagcgccttg
     4201 gaggcgccga gtaccctgcc tgagaggtcc ctggcctgca cgcccacgta gggctccgtg
     4261 gtacgcagaa cgatggaggt ctcgaagccc ttgcgcgggg cggatgcgac cacttccagg
     4321 cggtctgggc caggtcctgc caggacctgc caggttgcga cctccgtggc accgttccaa
     4381 cttgcgtaga gcgttacgtt gtcttcaggc ccgcgcttcg cgacgagcgc gggatcttct
     4441 tgtggctgcc cgctccacgg gaagcggaat gccctgtagg actcaccctc ggttgggaat
     4501 gcggcgctga agcggagcct gccatcgtgg ctgaactctg agaggacagg ctcgctgccc
     4561 caaccgacga agacgtttcc atcgggcaac acctgtacat tgccctgggt ggaggagcgg
     4621 accttgtcgg ggtgggtgta ttcgcgtacc agcctggctg tcattgcctc ctcgtccaac
     4681 tccaggacaa tggcgcgcga ctgatccact tcattcatgt tgccattgtc gaagacggtg
     4741 agtgtgccgt ccttctggcg tcgtgcgtcg tgttgaaagt tcgtccgcgt ccccgggccc
     4801 atctcgaaat cgctcctctt gccgccgagg cgccagatca cctctccgct tcgccggtcg
     4861 atcttgtaga tggtgcaggt cgtgcgggag gagacgagca ggttgccgtc gtggtccacg
     4921 tctattgagt tgaggtggaa gtagtcgtaa ggcaggacgt aagattcttc gagtcccacg
     4981 tgctccaggc tgtgccactc gaagagcaca tcaccgctct ctatgtccac ctcctggact
     5041 atcccgtcaa ggaccgtggc gcccttctcc cctccgtgct cggagaggtc catggtcacc
     5101 ttgtgataga tgtcgagcaa ggcggtgtcc tggggcgtga tcatgaactc gtggtggtct
     5161 gctctataga cgttgccagc cctgacccgc tttatctccc tgtaggcgtg gtcgcagatc
     5221 acgaactcac cctggccgta gctggtgtgg tggccctccc accaggtgag tacggtctca
     5281 cccttgtata tctgcacttt gaagtccata gcgtccattg cctcgcccgg agcgggacga
     5341 aaccacacgg gttggcccgt attgtcgagg ataaggcaac cgtcctgccc tggcccggac
     5401 tcacctgggc cgcttttcgg ggcgcagaag acgtatccag gagccgtgtc ctgtgcttgc
     5461 gtggtcaccc tgataacggg cggcttgagg tccggtcgcg agtggaaggc ccacgtttta
     5521 tctccctgcg cgggcgagga gttcgccctg gcagtagcct tcgcacgcgt ttcgggctcg
     5581 cagcccacgg ttccgcccag cgcgacccat gcggctccag cggtggccgc cccgaggaac
     5641 cttcggcgtg tgatcttccc tttcaagtcg ttgcgtcact tccttgt
//
LOCUS       DATAIR010000389         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_196019, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000389 DATAIR010000000
VERSION     DATAIR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196019"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VHM69_09530"
     CDS             <1..1081
                     /locus_tag="VHM69_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX2740677.1"
                     /translation="AALAPRLGATAAGVVSASHNPYPDNGIKFFSGEGRKLPSGKERE
                     IEDLFGQDISRPTGDGIGKVETLGDAPRLYAESVLERLRPRAEGVKVLLDCANGAAYE
                     VAPLVFGEIGADLTLTGTEPTGTNINEGCGSTHVEELDAAGHDVAFAFDGDADRVLAV
                     DEKGNVVDGDKIIAILARDLKVRGALAGEVIVTVMSNLGFFKAMEAMEIPYGVTSVGD
                     RHVAEEMARTGASLGGEQSGHVILSEHATTGDGIVTALALLDVMVRSGRPLSELAGVM
                     EVYPQTLINVRVEEPKAVATSQSVERAVSEAEKGLGDEGRVLLRPSGTEPVVRVMVEH
                     TDETVCREVCEEISRAVARAGEEGY"
     gene            1078..>1518
                     /locus_tag="VHM69_09535"
     CDS             1078..>1518
                     /locus_tag="VHM69_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740678.1"
                     /translation="MRFVGCALAWRPGEARDKVSCLVVMDERGSIIANSFAASAEDIA
                     GAVEGYGEERRGIVVGVDAPLAVPNERGTRRIERILSKVALPAYSASRRMFGGKPYSE
                     ELLSELEKVGVEYTDYPFPRERGQSAAVEVDSAATLKVLSLERSG"
BASE COUNT          264 a          434 c          583 g          237 t
ORIGIN      
        1 cgcggctctc gctccgcgcc tcggggcgac ggccgcgggc gtcgtaagcg cctctcacaa
       61 cccctacccc gacaacggca tcaagttctt ctcaggagaa ggccgcaagc ttccctccgg
      121 gaaggaacgc gagatcgaag acctgttcgg acaggacatc tcgcgcccga ccggcgacgg
      181 aatcggaaag gtggagacac tcggcgatgc cccgcggctg tacgcggaga gcgtgctcga
      241 gcggcttcgt ccgagggccg agggcgtgaa ggtgctcctt gattgcgcca acggggctgc
      301 ctacgaggtg gcgcctctcg tgttcgggga gatcggcgcg gatcttacgc ttactggaac
      361 agagcccacg ggcacgaaca tcaacgaggg ctgcggctcg acgcacgtcg aggaactgga
      421 cgccgccggg cacgacgttg cgttcgcctt cgacggggac gccgacaggg tgctcgccgt
      481 cgatgagaaa gggaacgtcg ttgacgggga taagatcatc gcgatccttg cccgcgatct
      541 gaaggtgcgc ggagcgctgg ccggcgaggt catcgtcacg gtgatgagca acctcggctt
      601 cttcaaagcg atggaggcga tggagatacc atacggggtg acgagcgtgg gggacaggca
      661 cgtcgccgag gagatggccc gtaccggtgc ctctcttggc ggggagcagt cggggcatgt
      721 gatcctctcc gaacacgcga cgaccgggga tggcatagtc acggcgctcg cgctgctcga
      781 cgtgatggtg cgcagcggca ggccgctctc cgagctggcg ggggtcatgg aggtataccc
      841 gcagacgctt atcaacgtga gggtagagga gccgaaggcc gtcgccacct cgcagtcggt
      901 cgagcgagcg gtctctgagg cggagaaggg gctcggagat gaagggcggg tcctcctgag
      961 gccgagcggg acggagcccg tcgtgcgggt gatggtcgag catacggacg aaacggtgtg
     1021 tagggaggtc tgcgaggaga tctcgcgggc cgtggcccgc gccggtgagg agggatattg
     1081 aggtttgtag gctgcgctct ggcgtggagg ccgggcgagg cgcgggacaa ggtctcgtgc
     1141 ctggttgtga tggacgagcg ggggagcatc atcgccaact cgttcgcggc gagcgccgag
     1201 gatatagcgg gggcggtcga gggctacggc gaggagcggc gaggtatcgt cgtcggcgtc
     1261 gacgcgccgc tcgccgtccc gaacgagagg gggacgcgca ggatcgaacg catcctctcg
     1321 aaggtggccc tcccggccta ctcggcgagc cgcaggatgt tcggtgggaa gccgtattcc
     1381 gaggagttgc tctccgagct cgagaaggtc ggcgtcgagt acacggatta ccctttcccg
     1441 cgcgagaggg gccagagcgc cgctgtcgag gtcgactcag cggcgacgtt gaaggtcctc
     1501 tcgctggagc gctcgggc
//
LOCUS       DATAIR010000390         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_242432, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000390 DATAIR010000000
VERSION     DATAIR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242432"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHM69_09540"
     CDS             <1..201
                     /locus_tag="VHM69_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2740679.1"
                     /translation="TDSNGTTYTYAVSEILEVPTTDLSVTNPVPGRDIVSLQTCIENY
                     GDYWTEGPNWFVRYVVRADKVS"
     gene            290..949
                     /locus_tag="VHM69_09545"
     CDS             290..949
                     /locus_tag="VHM69_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX2740680.1"
                     /translation="MEDYEWVWERFVRERRLEFGGHMDPDWQDGHRLSASLMVPVEAS
                     RFHDRLEPLREALRPFPFVSLHPDHFIHITLVLLGFLVEEPREEYEISHKRLEEIEGR
                     ARTALSDFPAFDIGLANLNAFPGAAFIETHDGGMIERLRDTLSVGCDLKKPHGPPHLT
                     IAYFEAPDGTEAPDELISAIARHRDWPVGEMTVENVEMTLIDLRSDYPKPRTLTRIPL
                     R"
     gene            complement(1083..1586)
                     /gene="sixA"
                     /locus_tag="VHM69_09550"
     CDS             complement(1083..1586)
                     /gene="sixA"
                     /locus_tag="VHM69_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX2740681.1"
                     /translation="MYLYLVRHAVAYSRDAERWPDDSKRPLTPEGEEEFRGAARGLDR
                     IVPEVEVLLSSPYERAWRTAEILAEQAGWPAPEVFPALEPDIPPDKFVLALESYAGKQ
                     SIAVVGHRPCLHELAVYLLTGDPGGADMKIKKGGVVCIEFDDAPKAGAGKLRWLFTPK
                     VLRSLSQ"
     gene            complement(1593..>3280)
                     /locus_tag="VHM69_09555"
     CDS             complement(1593..>3280)
                     /locus_tag="VHM69_09555"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX2740682.1"
                     /translation="TRQSKPGAIKSSATDHQELEWQFDVDDLEPVEGWLGQHFSGLGV
                     FPEDTVENTDTYYDTDDWRFYRAGYAMRVRKTNDGVEATMKSLSPAEGGLRRRREISE
                     PLKDDRPATLRKARGPVGKRLKVLLGGREINPLFQVQTRRQRFALLLKNSNDGDLEDV
                     RIGEVALDSSEFGDEPERLKRVEVEAVGGTSPDLHGFVDEMQFALELAPATISKYGTG
                     LLASGLNPDGGESFGKTDVDPSMSVGEVAFAVLRRQFVEMRNHEPGTRLGEDPEELHD
                     MRVPTRRMRAAMKVFQDALPERARWLREELRWVAHALGDVRDLDVQIERFQAWKEEAD
                     EESAEFLDRILKITEKRRVEARKNMLDVLESGRYERLESSFAEMLRRGPEEELAQSNG
                     HDPAREKITAAAPPLVSRRYRTWRKAAKRLDENSSPEAFHDVRKKGKRLRYTLEFVSE
                     VYGKPVQRLVKPLKALQDDLGDHQDAVVTAGFLRELGTKTDGTRVPRGAAFTMGVYSE
                     RCTREAADLRSSVPRSKPFRALTKGKRWKRFERIMQEARKNPNVAEPSSKSVR"
BASE COUNT          555 a         1081 c          993 g          651 t
ORIGIN      
        1 accgactcca acggcaccac atacacctac gcggtaagcg agatcctcga ggtgccgacg
       61 accgacctct cggtgacgaa cccggtgcca ggcagggata tagtttctct gcagacgtgc
      121 atagagaact acggtgacta ctggaccgag ggtcccaact ggtttgtccg ctacgtcgtc
      181 cgcgctgaca aggtctctta gagactacac gggccaggtt ccgccgccgt tccgctcccg
      241 cgcagattcg cgggagcggg tgtctcttac tctctgttag agtttccgga tggaggacta
      301 cgagtgggtg tgggagcgct tcgtccgcga gcgccgtctg gagttcggcg ggcacatgga
      361 ccccgactgg caggacgggc acaggctctc ggcctccctc atggtccctg tcgaagcgtc
      421 tcggtttcac gaccgcctcg aacccctgag agaagcgctt cgtccgttcc cgttcgtctc
      481 gcttcaccct gaccacttca tacacataac gcttgtgctt ctcggctttc tcgtggaaga
      541 accacgggag gagtatgaga tctcccacaa gagactagag gagatcgaag gtcgagccag
      601 gaccgccctc tcggacttcc ccgccttcga catcggactc gcgaacctca acgctttccc
      661 cggcgcggcg ttcatcgaga cgcacgacgg agggatgatc gaaaggttgc gggacaccct
      721 gagcgttggc tgcgacctga agaagcccca tggcccgccg cacctgacca tcgcctactt
      781 cgaggctcct gacggcacgg aggcgcccga cgagctcatc tccgccatag cacgccatag
      841 ggactggccc gtcggggaga tgacggtgga gaacgtagag atgaccctga tcgacctacg
      901 ttctgactac cccaaacccc ggacgctaac ccggataccg ctgagatagc atttcagcta
      961 gccagcacgg cgcttcgcgc ctttcagact tcagctcttc ctcgaacggc agtctccgta
     1021 tctctacacc cttgctggca ggctgagaag ctgacgagcg gaggccggag cgtgctgaca
     1081 cgctactgtg acagcgagcg aaggaccttc ggcgtgaaca gccagcgaag tttacctgct
     1141 ccggccttcg gggcgtcgtc gaactctatg cagacgacac cgcccttttt gatcttcata
     1201 tccgcgcccc cgggatcgcc ggtgagcagg tacacagcca gctcgtgcag gcagggtctg
     1261 tgtccgacca cggcgatgga ctgcttcccc gcgtaggatt ccagcgcgag gacaaacttg
     1321 tcgggcggga tgtcgggctc cagggccgga aacacctccg gtgcgggcca gccggcctgt
     1381 tctgcgagga tctcagcggt cctccacgcc cgttcgtacg ggctgctcaa aagaacctct
     1441 acctcaggga cgatgcggtc cagcccgcgg gccgcccccc tgaactcctc ctcgccttcc
     1501 ggcgtgagcg gacgtttact gtcgtcgggc cagcgttccg cgtcacggct gtaggcgacg
     1561 gcgtggcgaa cgaggtagag gtacacgcgg gcttaccgaa cgctcttgct cgatggttcg
     1621 gccacgttcg ggttcttgcg tgcttcctgc atgatcctct cgaaccgctt ccatctcttg
     1681 cctttcgtca gggcgcggaa gggtttcgaa cgaggcacgc tggagcgaag atcggccgcc
     1741 tcgcgggtgc agcgttccga gtagacgccc atcgtgaagg cggccccgcg tggtactcgc
     1801 gtaccatcgg tttttgtgcc gagctcccgc aggaatccgg ctgtgacgac ggcatcctgg
     1861 tggtcgccga ggtcgtcctg cagcgctttc aggggtttga ccagcctctg cacaggtttg
     1921 ccgtagactt cggacacgaa ctccagggta tagcgaagcc gtttcccctt cttgcgcacg
     1981 tcgtggaaag cctcggggga tgagttctcg tcgaggcgct tcgcggcctt cctccacgta
     2041 cggtagcgtc gggagaccag gggtggtgcg gcggctgtga tcttctcgcg cgcagggtcg
     2101 tgaccgttgc tctgcgcgag ttcctcctcg gggccgcggc gaagcatctc cgcgaacgaa
     2161 gactccagac gctcgtaccg gccggactcg agaacatcga gcatgttctt gcgggcctcc
     2221 acccgccgct tctccgttat cttcaggatc ctgtccaaaa actcagcgct ctcctcgtcg
     2281 gcctcttcct tccaggcctg gaagcgttcg atctggacat ccaggtccct gacgtcgccg
     2341 agggcgtgcg ccacccagcg taactcctcc ctgagccagc gggcccgctc gggcaacgca
     2401 tcctggaaga ctttcatcgc cgcccgcatc ctgcgcgtcg ggacgcgcat gtcgtgcagt
     2461 tcttcggggt cctcgccgag ccgcgtaccc ggctcgtggt ttctcatctc gacgaactgt
     2521 ctcctgagca ccgcgaacgc gacctcgccc acgctcatgg aagggtctac gtcggtcttg
     2581 ccgaacgact cgcccccatc cgggttgagc ccgctggcga ggagccctgt cccgtacttg
     2641 gaaatagtgg cgggtgccag ctcaagggcg aactgcatct cgtcaacgaa gccatgcagg
     2701 tcagggctcg tgccgcccac tgcctcgacc tcgacgcgct tcagccgctc tggctcatcg
     2761 ccaaactccg aggagtcgag cgcgacctcg ccgatgcgga cgtcctccag atccccatca
     2821 tttgagtttt tgagcaagag cgcgaacctc tggcggcggg tctggacctg gaacagtgga
     2881 tttatctcgc gcccgccgag gagcaccttc aaccgcttgc cgacggggcc acgagccttc
     2941 ctgagcgttg cgggtctatc gtcctttagc ggctcggaga tctctcgccg cctccggaga
     3001 ccaccctcgg cgggcgatag cgacttcatc gttgcctcca cgccgtcgtt agttttgcgg
     3061 acgcgcatgg cgtagccagc cctgtagaaa cgccagtcgt cggtgtcgta gtaagtgtcg
     3121 gtgttctcca cggtatcttc agggaagacg cccaagccgg agaagtgctg gccgagccag
     3181 ccctcgacgg gctcgagatc gtcgacgtcg aactgccact ctaattcctg gtgatccgtg
     3241 gcggaggact tgatcgcacc tggtttgctc tgtcgcgttg
//
LOCUS       DATAIR010000391         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_118004, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000391 DATAIR010000000
VERSION     DATAIR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118004"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHM69_09560"
     CDS             <1..307
                     /locus_tag="VHM69_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2740683.1"
                     /translation="DAIKEFVDRWGQRGMARGAVVVIFSDGWDRGDVSVLAEQMQRLS
                     RLAYRIVWVNPLKAAPGYKPLAGGMAAALPHVDVFLSGHNFESLEELARAIAAATGR"
     gene            379..693
                     /locus_tag="VHM69_09565"
     CDS             379..693
                     /locus_tag="VHM69_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2740684.1"
                     /translation="MNPVIDRIAGWREEGKKAALATVVKVERSGPLGPGTSMAVSEDG
                     QVVGSVSGGCVEPAVYEEAMAAMESGQPKLLSYGISDDEAFGVGLTCGGIIHIFVERV
                     DW"
     gene            693..1493
                     /locus_tag="VHM69_09570"
     CDS             693..1493
                     /locus_tag="VHM69_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX2740685.1"
                     /translation="MADLLSRWHAALTDQSPVAIATVIQGPAGSLGGKILVSPEDHTG
                     TAGNDDLDRAIVEGARAMLEGGQTGTVHYGPRGQRRMEDVTVFIQSFAPPPRMYVFGA
                     IDFASAVARVGKFMGYRVIVCDARAVFATRERFPSADEIVVAWPDEFLKTAEVDSRTV
                     ITVLTHDPKFDVPVLKEALNTPAGYIGAMGSRRTHDNRTARLKEEGVTDEQLARISSP
                     IGLDIGARTPEETAIAIAAEIVALRTGHSGGRLAERSGPIHAARENIR"
     gene            1620..2492
                     /locus_tag="VHM69_09575"
     CDS             1620..2492
                     /locus_tag="VHM69_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX2740686.1"
                     /translation="MQVPARFEYEVAGTVGEAVELLGRYGEGAYLIAGGHSLIPMMKL
                     RLAVPDVLIDISGLDDELRYIEEDGGTLRIGALTRHREVLESPLIRERYALLADAERM
                     IADPLVRNMGTIGGDLANADPAEDLPAAFVALDSEVVAHGPDGERTVHVDDLYVGPYE
                     TSLDHGEVLTEVRVPRSPGGSAYSKVKRKTGDYASAAIGVALSMTGGEIESIRIGMCG
                     VGSRTLRATGAEEALTGERPGSEAYRRAGDIAAEECDPIDDAKGTPEYKRDLVRVLLG
                     RTVARAVERAEVQS"
     gene            2489..2968
                     /locus_tag="VHM69_09580"
     CDS             2489..2968
                     /locus_tag="VHM69_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2740687.1"
                     /translation="MRITVNVNGVMLEAEVEPRLLLVHFIRETLGLTGTHWGCDTSNC
                     GACTVLLDGEPVKSCTVLAVRANNHEVTTIEGLWSGTELTPVQEGFHVEHGLQCGFCT
                     PGMIITATEFLERNPDPTEAEIREAISGNLCRCTGYENIVKAVQWAARNGNTQEVGA"
     gene            2968..>3335
                     /locus_tag="VHM69_09585"
     CDS             2968..>3335
                     /locus_tag="VHM69_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX2740688.1"
                     /translation="MATAQGHTLGDRMPRKEDGRFIRGKGHYVDDVRLPGMLHSAILR
                     SPFAHARINSVDKRSALELPGVHAVITGNDLDAQGLAWMPTLAGDTQAVLATDKVRFQ
                     GQEVAFVVADDIYIAQDALEL"
BASE COUNT          638 a         1012 c         1168 g          517 t
ORIGIN      
        1 ggatgcgatc aaggagttcg ttgaccgctg ggggcagcgt ggcatggcgc gcggtgccgt
       61 cgtcgtgatc ttctccgacg gctgggacag gggcgacgta tccgtgctcg ccgagcagat
      121 gcagcgcctg agccgcctcg cctacaggat cgtctgggta aaccctctca aggccgctcc
      181 cggctacaaa cctctggccg gcggcatggc cgcggcactc ccacacgtag acgttttcct
      241 ctcaggccac aacttcgagt ccctcgaaga gctcgcccgc gccatcgccg ccgcaacagg
      301 gcgatagaat gaaggcttca ggctacaggt tttcctgatg cctgaagcct gacacctgcc
      361 gaccgaaggg aggctcttat gaacccggtc atagatagga tcgcgggctg gcgcgaggag
      421 ggcaagaagg ccgccctagc cacggtggtg aaggtcgagc ggtcagggcc tctggggccg
      481 ggcacttcga tggccgtctc ggaggacggg caggtcgtgg gatccgtgag cggggggtgc
      541 gtggagcctg ctgtctacga ggaagcgatg gcggcgatgg agagcggtca gccgaagctc
      601 ctctcctacg gcatctcgga cgacgaggcg ttcggggtgg ggctgacgtg cggtgggatc
      661 atccacatct tcgtcgagcg cgtggactgg tgatggctga cctgctctcc cgctggcacg
      721 ccgcactgac cgatcagtcg cccgtggcta tcgcgacggt gatacagggt ccggcgggtt
      781 ccctcggagg taagatcctg gtctccccgg aagaccacac aggtacggcg ggaaacgacg
      841 acctcgacag ggccatcgtc gagggcgcgc gggcgatgct cgaaggcggc cagacaggga
      901 ccgtccacta cggaccacgc ggccagcgcc ggatggagga tgtgacggta ttcatccagt
      961 cattcgcccc gccgccgagg atgtacgtct tcggagccat agacttcgct tcggcggtcg
     1021 ccagggtggg caagttcatg ggttaccgcg tgatcgtctg cgacgcccgc gccgtcttcg
     1081 ccacccgaga acgcttccct tcggcagacg agatcgtcgt cgcctggccc gacgagtttc
     1141 tgaagaccgc cgaggtagac agccgcaccg tcatcaccgt ccttacccac gacccgaagt
     1201 tcgatgtgcc cgtcctgaag gaagcgctga acacaccagc gggctacatc ggggcgatgg
     1261 ggagccgaag gacgcacgac aaccgcacgg cgcgtttgaa agaagaaggc gtcaccgacg
     1321 agcagctcgc gcgcatcagc agccccatcg gcctggacat cggggcgcgc acgccggagg
     1381 agacggccat cgccatagcg gcagagatcg tcgcgctgag gacggggcat tcgggcggac
     1441 gcctcgcaga acgctcgggg ccgatccacg cggcaaggga aaatatccgg tgagctgaga
     1501 tcgcgaaatc cacgcgctga gtgtggacgg ggtaaaataa ttcgccaggg gattgtgctc
     1561 cggttctggg ccggaggaaa gggacaactc caacaccgtt ggggagaaag gagggccgcg
     1621 tgcaggttcc ggcgcggttt gagtacgagg tcgctggcac cgtaggggaa gcggtcgagc
     1681 ttctggggag gtatggagaa ggggcctacc tgatcgccgg tggccacagc ctcatcccga
     1741 tgatgaagct caggctcgct gttcccgacg tcctcataga cattagcggg ctagatgacg
     1801 agctgcgcta catcgaggaa gatggcggga ctttgaggat cggggcgctc accaggcacc
     1861 gcgaggtgct cgagtctccg ctcatcaggg agcggtacgc gctgctcgcc gacgcggaga
     1921 ggatgatcgc cgacccctta gtaaggaaca tggggactat cggcggagac ctcgccaacg
     1981 ccgacccggc ggaggatctc ccggcggcct tcgtggccct ggacagcgag gtcgtcgcac
     2041 acggccctga cggggagcgt acagttcacg tagacgacct gtacgtcggt ccttacgaga
     2101 cgtccctgga tcacggcgag gtcctcacgg aggtcagggt gccgcgctct cctggaggga
     2161 gcgcctactc caaagtcaaa cgcaagacag gagactacgc ctccgccgcg atcggcgtcg
     2221 ccctgtccat gaccggcggg gagatagaga gcattcgtat cggcatgtgc ggcgtcggga
     2281 gccggacctt gcgggcgacg ggcgcggaag aggcgctcac gggggagcgt ccggggtccg
     2341 aggcgtacag gagggcagga gacatcgctg ctgaggagtg tgatcccata gacgacgcca
     2401 aaggcacgcc ggagtacaag agggaccttg taagggtgct ccttggacgg acggtggcca
     2461 gggcggtcga gcgcgcggag gtacagagtt gaggataacg gtcaacgtaa acggcgtgat
     2521 gctcgaggcc gaggtcgagc cgcgcctctt gctcgtccac ttcatcaggg agaccctggg
     2581 gctcacgggt acccactggg ggtgcgatac gtccaactgc ggtgcatgca cggtgctcct
     2641 cgacggggag cctgtcaaga gctgcacggt gctcgccgtc agggccaaca atcacgaggt
     2701 gacgaccatc gagggactct ggagcgggac agagctcacc cccgtgcagg aaggctttca
     2761 cgtcgagcac gggctgcagt gcggcttctg tacgcctggc atgatcataa ccgcgacgga
     2821 gttcctggag cgcaaccccg acccgacgga ggcggagatc cgggaggcca tctcgggcaa
     2881 cctctgccgc tgcaccggct acgagaacat cgtgaaggca gtccagtggg cggcgagaaa
     2941 cggcaacaca caggaggtcg gcgcgtaatg gccacggcac agggacatac tctcggcgac
     3001 cggatgcccc gcaaggagga cgggcgtttc atcaggggca agggtcacta cgtcgacgac
     3061 gtcaggctgc cagggatgct gcactcggcc atcctgcgca gccccttcgc ccacgccagg
     3121 ataaatagcg tggataagag atccgcgctc gagcttcccg gcgtccacgc tgtcatcacc
     3181 ggcaatgacc tcgacgcgca gggcctcgcc tggatgccca ccctcgccgg ggacacccag
     3241 gccgtgctcg ctacggacaa ggtgcgcttc caggggcagg aggtcgcctt cgtggtggcc
     3301 gacgacatct acatcgcgca ggacgcgctg gagct
//
LOCUS       DATAIR010000392         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_25050, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000392 DATAIR010000000
VERSION     DATAIR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25050"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VHM69_09590"
     CDS             complement(<1..1182)
                     /locus_tag="VHM69_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX2740689.1"
                     /translation="MKVGIPREVNEGERRVGLVPETVGRLVKDGFEVIVESGAGRDYN
                     LDADYEEAGAKVVEGAEAVYGEADIVVKVAKPTDDEIGQMHDGLLLICFLNGPQDPEL
                     VKQLAEAGVTVFSNEQIPRTSVAQSMDALSSMGSIAGYKCALIAADTLGKYVPMLSTA
                     AGTTKAANVLVLGVAVAGLQAIAIMNRLGGEVFAYDIRPETKEQAQSLGASFIDSDDE
                     KEDEEEDQFEEYKPTGWRGLMVRLGFYSFGEPPRDQYVVEGEEEDEEEEEEEGWSQEK
                     LEEDQKLLRERMKEMDIVITTALVPGRQAPKLVDKDMVESLKPGSVIVDLAAESGGNC
                     ELTEPGETVEHEGVKILGPLNLPSAMPIHASQLLSRNMYNLISHITEDRAEDKDEKDP
                     QL"
     gene            1333..2007
                     /locus_tag="VHM69_09595"
     CDS             1333..2007
                     /locus_tag="VHM69_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2740690.1"
                     /translation="MKKIRSGYTMKTLTALVLAVFLFVGLGLLGYSMFPGGSPASADS
                     PGRVHGRISQELEKKFQRSIPERIVEKIRGPRVETPESKLLKLTVPDLERVDEVPVYD
                     TPKSGYEEALHDGLVHVRGTQFPWQKEANVYIGGHRVGYPGTRSNLVFWDLNKLEKGD
                     KIFLTDAEGTRYTYEVFRKFTFGPKDGRFMLPLEGKNIVSLQTCTLPDYSHRLIVQGE
                     LKGKTK"
     gene            2116..2697
                     /locus_tag="VHM69_09600"
     CDS             2116..2697
                     /locus_tag="VHM69_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2740691.1"
                     /translation="MKTYKTRRSSGRMLGSLVATLLVASGLILVAYSFLIGDPLAGAA
                     VFAHKPPGNSTMSLTVPEMQRVDDVPVYTGAASNETALHDGTLHLNGTGFPWQTGSNV
                     YIAGHRMGFPATKSYLVFWDLNKLEDGDEVILTDANGTQYTYSVFKSFVVNPDASYVT
                     QPVPGKSVVSLQTCTLPDYSQRLIVQAELTDVS"
     gene            complement(2815..3279)
                     /locus_tag="VHM69_09605"
     CDS             complement(2815..3279)
                     /locus_tag="VHM69_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX2740692.1"
                     /translation="MIYSRTQREPLIFGHNTRREPESEPEVDISKKSKRKGKGGALAT
                     LTRPDAKVRDYDSHVLVCKGGDCKKRGSKAVRGTLKDALRAEGMNRDVRVDSVDCLGL
                     CKHGPNLVVYPPGTWYLGVIVEDVPELVQQHLKNGEPVEHLAAELRPRKKSK"
     gene            3340..4212
                     /locus_tag="VHM69_09610"
     CDS             3340..4212
                     /locus_tag="VHM69_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740693.1"
                     /translation="MTRYSVVDVGSNTIHLLVGEVDEGSVIPVTGEKVSARLGAGVEK
                     TGRLDEERLALATEAITLFARIAALHGAPSPEVLATSAVRDAENGLELVERVREATGL
                     KMRLISGETEAALGFRGALSAVRSEREVLVVDLGGGSAQLILGETSAEERVSLPLGTN
                     RTTERFVVQDPPVDAELEALREHAARLLPEWDIGGAQVVAVGGSARAILKITPDRLTA
                     DRLWRLSAEIRKHPSGVLARDQGLAPERARVLPAAITTFATILEHFSKEDLTVARGGI
                     REGTLLALAEGEEI"
     gene            4209..>5274
                     /locus_tag="VHM69_09615"
     CDS             4209..>5274
                     /locus_tag="VHM69_09615"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX2740694.1"
                     /translation="MTDVARRPSLSDPSLYINRELSWLGFNNRVLEQARDERHPLLER
                     VRFVSISETNLDEFFMIRVAGLQQQVASELPNPVPDGMTPEEQLSRIHDHTEEFFEER
                     RRILNKELMPALEEEGIRMVPHGKVRAAERRELREKFVRDILPILTPLAIDPAHPFPH
                     ISNLSLNLLVVIEDDGRRVMARIKVPTTIDRFMRLPDKDRGPEERPEIRFVRVEEVIA
                     ANLDELFPGKEVVASYVFQVTRNADFVIEEDEASDLLQAIEDELESRWFGQSVRLVVS
                     DEMPDDLREWLAGNLKLNENSVFAAPHPIGLADLEELTHLERSDLLYPPITPRVPSEI
                     RSARSITQAIRNGDILLYHPY"
BASE COUNT          992 a         1622 c         1643 g         1017 t
ORIGIN      
        1 gagttgaggg tctttctcgt ctttgtcctc tgccctgtcc tcggtgatgt gggagatgag
       61 gttgtacatg ttgcgcgaga ggagctggct cgcgtgtatc ggcatggcac tgggcaggtt
      121 gaggggaccg aggatcttca ccccttcgtg ctcgacggtc tccccaggct cggtcagctc
      181 gcagttgccg ccgctctccg cagcgaggtc gacgatgact gatccgggct tgaggctctc
      241 gaccatgtct ttgtccacaa gtttgggagc ctgcctgcca gggacgagcg ccgtggttat
      301 tacgatgtcc atctccttca tgcgctcgcg caagagcttc tggtcctcct ccagcttctc
      361 ctgggaccac ccctcttctt cctcttcttc ctcatcctct tcttcgcctt ccacgacgta
      421 ctgatccctc ggcggctctc cgaatgagta gaacccgagc ctgaccatga gcccgcgcca
      481 gcccgttggc ttgtactcct cgaactgatc ctcttcttcg tcttctttct cgtcgtccga
      541 gtcgataaag gaggcgccga gcgactgcgc ctgctctttg gtctcgggac ggatgtcgta
      601 ggcgaagacc tcgccaccga gcctgttcat gatggctatg gcctgcaaac cagcaacggc
      661 caccccaagc acaagcacgt tcgccgcctt ggtggtcccc gccgcggtcg agagcatggg
      721 cacgtacttg cccagagtat cggcggcgat gagggcgcac ttgtagccag cgatggaacc
      781 catcgaggag agggcgtcca tgctctgcgc gacgctggtg cgtggtattt gctcgttgga
      841 gaaaacggtg acgccggctt ccgccagctg cttgacgagt tccgggtcct gggggccgtt
      901 gaggaagcag atcaggagaa gaccatcgtg catctggccg atctcatcgt cggtaggctt
      961 tgccaccttg acgacgatgt cagcctcgcc gtagaccgcc tcggcgccct ctacgacctt
     1021 cgccccagcc tcctcgtagt ccgcgtcgag gttgtagtcg cggcccgcgc cagactcgac
     1081 gatgacctcg aaaccgtcct tgacaagcct gccgacggtc tcgggaacca gcccgacccg
     1141 acgctccccc tcgtttacct cgcgcggtat tcctaccttc acgcggcaga ttatagcgtc
     1201 gaaaacggac acgcgctgac gcggggacaa tcgttatcaa caaagctccg ttttgtaatc
     1261 gaaatgtcac ctttggggct taaattccta ctatcgaccg tagtaactga tccagagagg
     1321 aggtcgagga cgatgaagaa gataaggagt ggatacacca tgaagacgct gacggcgctc
     1381 gtgttggccg tgttcctgtt cgtcgggctc ggcctgctgg ggtactcgat gtttccggga
     1441 ggttctccgg cctcggcgga tagccctggg cgggtacacg gcaggatctc gcaggaactg
     1501 gagaagaagt tccagaggag tatccccgag aggatcgtcg agaagataag gggtccgagg
     1561 gtagagacgc ctgagagcaa gctcctaaag ctcaccgtcc ccgacctgga gcgggtcgat
     1621 gaggtgccgg tctacgacac tccgaagagc gggtacgagg aggccctcca cgacggcctg
     1681 gtgcacgtca ggggcacgca gttcccctgg cagaaggagg ccaacgtcta catcggaggc
     1741 cacagggtgg gttatccggg gaccaggagc aacctcgtct tctgggacct caacaagctg
     1801 gagaaggggg ataagatctt cctgaccgac gccgaaggaa cccgctacac ctacgaggtg
     1861 ttcaggaagt tcacgttcgg ccccaaagat ggacgcttta tgctgcccct tgaaggcaag
     1921 aacatagtga gcctgcagac gtgtacgctc cccgactact cgcatcgcct catcgtgcag
     1981 ggcgagctta agggcaagac gaagtaaacg cccgggcaga gatccgttac cgacagggcc
     2041 ccattttgta acctgaatgt tactttcgtg gggtatatct ccacacgaag accggcggag
     2101 aggaggtaga agtacgtgaa gacctacaag actagaagaa gcagtggcag gatgctcggc
     2161 tccctcgttg ccaccctgct cgtcgcttcg ggcctgatcc tggtggctta ctccttcctc
     2221 ataggcgacc cgctcgccgg cgcggcggtc ttcgcgcaca agccccctgg caactcgacg
     2281 atgagcctga ccgtccccga gatgcaacgg gtcgacgacg ttcccgtcta cacaggcgca
     2341 gcgagcaacg agaccgcgct ccacgacggg acgctccacc tgaacggcac gggattcccc
     2401 tggcagacgg gctccaacgt ctacatagcg ggccaccgca tgggcttccc cgccacgaag
     2461 agctatctag tgttctggga cctgaacaaa ctggaggacg gcgacgaggt cattcttacc
     2521 gacgcgaacg gtacccaata cacctacagc gtcttcaaga gctttgtcgt caacccagac
     2581 gcctcctacg tcacgcaacc cgttccaggc aagagcgttg tcagcctgca gacgtgtacg
     2641 cttcccgact actcgcaacg cctgatcgtc caggccgaac ttacggacgt gagttagagc
     2701 ggaccgcgga agtctattcc gcgatccact ctgtcaggca tcagctttca gcctgtttgt
     2761 ttactgatga tggtagagat tctgaggtgg gtggaggtgc gagaccccgc tcctctattt
     2821 cgatttcttg cggggtctca actcggcggc tagatgttcg acgggttcgc cgttcttgag
     2881 gtgctgttgt accaactcgg gcacgtcttc cactatgacg ccgaggtacc aggtgccagg
     2941 ggggtagacc accaggttcg ggccgtgctt gcagagtcct aggcagtcca cggagtccac
     3001 gcggacgtct ctgttcatgc cctcggcgcg cagcgcgtcc ttgagcgtcc cgcggacggc
     3061 cttcgaaccg cgcttcttgc agtcaccacc cttgcagacg agcacgtggg agtcgtagtc
     3121 gcggaccttc gcgtcgggcc tggtgagcgt cgccagcgcg ccgcccttcc ctttgcgttt
     3181 cgacttcttc gagatgtcta cctccggttc cgattctggc tcgcgacgcg tgttatgtcc
     3241 gaagatcaga ggctctcttt gagttctcga atatatcacc ctgggcggcg ggactgtatc
     3301 atggatggta taagggttca cgacacgaag gacaatcgct tgacccggta ttcggtcgtc
     3361 gacgttggtt ccaacaccat ccacctcctc gtgggggagg ttgacgaggg ctcggtgata
     3421 ccggtcacgg gcgagaaggt ctcggccagg ctcggggccg gtgtggagaa gacgggtcgt
     3481 ctcgacgaag agcggctcgc gctcgcgacc gaggcgataa cgctcttcgc gaggatagcc
     3541 gccctgcacg gtgcgccatc gcccgaggtg ctggccacga gcgcggtccg cgacgccgag
     3601 aacggcctcg aactggtcga gcgggtacgg gaggccacgg gcctgaagat gcgtttgatc
     3661 tccggcgaga cggaggccgc gctgggtttc aggggcgctc tctcggctgt gcgcagcgag
     3721 agagaggtgc tcgtcgtcga cctcgggggc ggctcggcgc agctcatcct cggcgagacg
     3781 tccgcggagg agcgagtatc gctgccgctc gggaccaacc gcactacgga gcgtttcgtg
     3841 gtgcaggacc cccctgtgga tgcggagctc gaagcgttgc gcgagcacgc ggcgcggcta
     3901 ctgcctgaat gggacatagg cggggcgcag gtagttgcgg tcggcgggtc ggcgcgcgcg
     3961 atcctcaaga tcacacccga caggctcacg gctgaccggc tgtggcggct atccgcggag
     4021 attcggaagc atccgtccgg ggtgctggcc agggatcaag ggctcgcgcc ggaacgagca
     4081 cgggtcttgc ccgcggccat cacgacgttc gcgactatac tggaacactt tagcaaggag
     4141 gatctgaccg tggcacgcgg gggaatcagg gaggggacgc tgctcgcgct cgccgaaggg
     4201 gaggagatat gacggacgta gcacgaaggc caagcctttc cgacccgtcg ctgtacataa
     4261 acagggagtt atcgtggctc gggttcaaca acagggtttt agagcaggcc cgtgacgagc
     4321 ggcacccgct tctggagagg gtgcgcttcg tctcgatctc cgagaccaac ctcgacgagt
     4381 tcttcatgat ccgggtcgct ggcttgcagc agcaggtcgc ttcggagctt ccaaaccctg
     4441 ttccggacgg gatgaccccg gaggagcagc tctcccgcat ccacgatcat acggaggagt
     4501 tcttcgagga gcggcgcagg atactcaaca aggagctcat gcctgccctg gaagaagagg
     4561 gcatacgcat ggtgccccac ggtaaggtcc gcgccgcaga gcgacgggaa ctcagggaga
     4621 agttcgtcag ggacatactg ccgatactca cgcccctcgc catagacccc gcccacccct
     4681 tcccgcacat ctccaacctc tcgctgaacc tgctcgtcgt gatagaggac gatggcaggc
     4741 gggtgatggc ccgcataaag gtgccgacca ccatagaccg tttcatgagg ctccctgaca
     4801 aggaccgcgg tcccgaggaa aggccggaga tccgcttcgt acgggtcgag gaggtcatcg
     4861 cggcgaacct ggacgagctc ttcccgggca aagaggtcgt cgccagttac gtcttccagg
     4921 ttacgcgcaa cgcggacttc gtgatcgagg aggacgaggc ttctgacctc cttcaggcca
     4981 tagaggacga gctcgagagc cgctggttcg gccagagcgt caggctcgtt gtgtcggacg
     5041 agatgccgga cgacctgcgg gaatggcttg caggcaacct caagctcaac gagaactcgg
     5101 tcttcgccgc gccccatccc atagggctcg ccgacctgga ggagctcacg cacctcgagc
     5161 gctcggacct gctatatccc ccgatcaccc cccgggtgcc ttccgagatc cggagcgcgc
     5221 gttctatcac ccaggcgatc cgcaacggcg acatacttct ctaccatccc tacg
//
LOCUS       DATAIR010000393         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_196096, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000393 DATAIR010000000
VERSION     DATAIR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196096"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VHM69_09620"
     CDS             complement(<1..973)
                     /locus_tag="VHM69_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2740695.1"
                     /translation="MPEDLAIFIDWENIYISTVTEYKSKPNVSAILEKSREYGRIVSA
                     TAYADWTDGDFRQAPPTLYSNGISPRYISARYFPGGKSQKRRTNSIDVMLAVECSDFL
                     HNHPQVDTYVLVTGDGDFIPLVSLLRSKGKIVVVIGVSEATSYHLIESADHFISYASL
                     LEEDRAARAQDKEPKKVEADPFDELVRAVETLKRGGKTRVLGQVKQQMIVQLGSFDER
                     NLGFKKFKDFVVEAERRDLVNTVDQDLELQVYLPDEKVPDLQEADLAAEDPKETASSN
                     RSKSNGKPAREASETEDDFDLLEDLTPYELKTVCTSVASLEQPATFVE"
     gene            complement(1030..1443)
                     /locus_tag="VHM69_09625"
     CDS             complement(1030..1443)
                     /locus_tag="VHM69_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740696.1"
                     /translation="MHSECEGVVECTALVLAESCTEYEDRLTLFGVLDEIRPSETGET
                     SFVVYTKFEGCEHASDKERLARILVTDEDGEVLMESAEYSVYLDGTEEPVTIVAAFDL
                     PEGFDVGEQLLWVEAVLDEETLSQTVVRIRDRYSV"
     gene            complement(1436..>3059)
                     /locus_tag="VHM69_09630"
     CDS             complement(1436..>3059)
                     /locus_tag="VHM69_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX2740697.1"
                     /translation="PKVVLNNLYKHTQMQQSFGVNLVALVDGVPKTLSYKQVLKHYVA
                     HQFDVVTRRTRYELRRAQARAHILEGLLIALSNLDEVVATIRRSRSVETARKNLMKAF
                     KLSERQAQAILDLRLQRLTAMERQKVEQEHRDLIEKIEYLESVLADESKVYGIVKEEL
                     AEVKAAYADDRRTVITAEEGVDFEIEDLIAEEEMVISISNGGYLKSVPVNTFRKQGRG
                     GVGVAGMELKEGDYIEHLFITTTHHYMLFFTNRGKVYRLKVHQLPRMGRTAKGRHIAN
                     LLPFAQGERIQSVIATKEFNEAEYLLFATKNGIVKKTNFLEYNTPLKSDGIIALNLAD
                     DDELISVRQVSEGADVVIVSRNGKAIRFRQTDARPMGRATAGVKGITLKNDDVVLSMD
                     VVSDDAADLFMITERGLGKRTALSGFPTHGRGGQGVIAMKTDGERGKLAGVRVVRPGL
                     HELMIVSNFGTTIRMDADSVSRQGRAAQGVRVMNLRSGDSVSAIAKVVSSRSTGADGA
                     TDELALDEISGEEQIGTSTAGLPEPNGKVPDA"
BASE COUNT          490 a          962 c          952 g          655 t
ORIGIN      
        1 tctccacgaa agtggcaggc tgttcgagag aggcgacgct cgtacagacc gtcttgagct
       61 cgtacggagt gaggtcttcg aggaggtcga agtcgtcttc cgtttctgac gcctcccgag
      121 cgggcttgcc gttactcttg ctccggttcg aggacgcggt ttccttcggg tcctcggctg
      181 cgaggtcggc ctcctggagg tcgggtacct tctcgtcggg caggtagacc tggagttcga
      241 ggtcctggtc caccgtgttc accaggtcgc ggcgctcggc ttcgacaacg aagtccttga
      301 acttcttaaa gcccaggttg cgttcgtcga aagatccgag ctgcacgatc atctgctgct
      361 tcacctgacc gagaacacgc gtcttgccgc ccctcttcag cgtctcgacg gcacgcacga
      421 gctcatcgaa aggatctgct tccaccttct tcggttcctt atcctgggcg cgggccgcgc
      481 ggtcttcctc caggagggag gcgtaggaga taaagtggtc ggcagattcg atcaggtggt
      541 acgaagtcgc ctcggagacc ccgatcacga cgactatctt ccccttgctc ctgagcaggc
      601 tcacgagcgg gatgaagtcc ccgtcgcccg tgacgagcac gtaggtgtca acctgggggt
      661 ggttgtgcag gaaatccgag cactcgaccg caagcatcac gtcgatggag ttggtgcggc
      721 gcttctggga cttgcccccg gggaagtacc gggccgagat gtagcgcggc gagatgccgt
      781 tggagtagag ggttggcggg gcctggcgga agtccccgtc ggtccagtcg gcgtaggccg
      841 tcgcagagac gatccgcccg tactcgcggg atttctcgag gattgcagag acgttcggct
      901 tcgatttgta ctccgtgacc gtggagatgt agatgttctc ccagtcgatg aatattgcca
      961 gatcttcagg catgaatagg tttcctaacg tagaggagag gcccggagat ccgggcctca
     1021 ggtggtctct tatacactat aacggtcgcg gatcctgacc acggtctggg agagtgtctc
     1081 ctcgtcgagg accgcctcga cccagagcag ttgctccccg acatcgaagc cctcgggcag
     1141 gtcgaaggcc gcaactatcg tcaccggttc ctccgtcccg tcgagataga cggagtactc
     1201 cgccgattcc atcaggacct cgccgtcctc gtcggtgacg aggatgcgcg ccaggcgctc
     1261 cttgtccgag gcgtgttcgc agccttcgaa cttggtgtag acgacgaaag aagtctcgcc
     1321 cgtctcgctc gggcggattt catcgaggac cccgaagagc gtgagcctgt cttcgtactc
     1381 ggtacagctc tcggcgagga cgagggccgt gcactcgacc acgccttcgc actcgctatg
     1441 catccggcac cttcccgttc ggttcgggaa gccctgcggt cgatgtacca atctgctctt
     1501 cgccggagat ctcatccagc gccagctcgt ccgtcgcgcc gtcggcgcct gtggagcgcg
     1561 aggagacgac cttggctatg gcgctcaccg agtcgccgct tctcaggttc atgactcgca
     1621 cgccctgcgc cgcgcggccc tgacgcgaga cggagtcggc gtccatgcgg atggtcgtcc
     1681 cgaagttgga gacgatcatc agctcgtgca ggccaggacg aacgaccctc acgccggcga
     1741 gtttgccgcg ttcgccgtcg gtcttcatcg ctatgacgcc ttgtccgccg cgcccgtgcg
     1801 tcgggaaccc ggacagggcc gtgcgcttgc ccaggcccct ctcggtgatc atgaacaggt
     1861 cagccgcgtc atcggagacc acgtccatgc tcaggacgac atcgtcgttc ttgagcgtga
     1921 tccccttaac cccagcggtg gcccggccca taggccgcgc gtccgtctgc ctgaagcgga
     1981 tggctttgcc gttgcggctc acgatcacca cgtcggcgcc ttcagagacc tgccgcaccg
     2041 agatcaactc gtcatcgtcc gcgaggttga gtgcgatgat cccgtcgctc ttcagcggcg
     2101 tgttgtactc gaggaagttc gtcttcttga cgatgccgtt cttcgtcgcg aagagcaggt
     2161 actcggcctc gttgaactcc ttggtcgcga tgacgctctg gatcctctcc ccctgcgcga
     2221 acggcagcag gttggcaatg tggcggccct tcgccgtgcg gcccatcctc gggagctggt
     2281 ggaccttgag gcggtacacc ttgccgcggt tggtgaagaa gagcatgtag tgatgcgtcg
     2341 tcgtgatgaa gaggtgctcg atgtagtcgc cctctttgag ctccatcccc gcgaccccca
     2401 cgcctccgcg accttgcttg cggaaggtgt tgacggggac gctcttcagg tagccgccgt
     2461 tggagatgga gatgaccatc tcctcctcgg ctatgaggtc ctctatctcg aagtccacgc
     2521 cctcctcggc cgtaatgacc gtcctgcggt cgtccgcgta ggcagccttg acctcggcga
     2581 gctcctcctt gacgatcccg tacaccttgg actcatcggc caggacgctc tcgaggtatt
     2641 cgatcttctc gatgaggtcc ctgtgctcct gctcgacctt ctgccgctcc atcgccgtga
     2701 gccgctgaag gcggaggtcc aatatcgcct gcgcctggcg ttcggagagc ttgaaggcct
     2761 tcatcaggtt cttgcgcgcc gtttcgacgc ttcggctcct gcggatggtc gccacgacct
     2821 cgtccaggtt ggagagggcg atgagcagtc cttcgaggat gtgcgccctc gcctgcgccc
     2881 tccgcagttc gtagcgcgtc ctgcgcgtga ccacgtcaaa ctggtgcgcg acgtagtgct
     2941 tgagtacctg cttgtacgaa agcgtcttcg ggacaccgtc cacgagcgca accaggttca
     3001 cgccgaagct ctgctgcatc tgggtgtgct tgtagaggtt gttgaggacg accttgggc
//
LOCUS       DATAIR010000394         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_334234, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000394 DATAIR010000000
VERSION     DATAIR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334234"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..996)
                     /locus_tag="VHM69_09635"
     CDS             complement(355..996)
                     /locus_tag="VHM69_09635"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX2740698.1"
                     /translation="MRATLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRNILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDDNPEKIGRTVGNVVVKHIDEFKKSVAEAEEIIGIIATPASSAQEV
                     ANLMVDADIRVILNYTDVLLHVPSHVDVHRIDPTAQLMHTLYYLTQAEGQEAS"
     gene            1230..1976
                     /locus_tag="VHM69_09640"
     CDS             1230..1976
                     /locus_tag="VHM69_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX2740699.1"
                     /translation="MVVLVAVAVALAGLIPAQRLVPFSVRESHNIHTAAMFGALYVVY
                     GLMVGFSAYLVAVQFDAAQQTVENEANSVEGIYLLAEQFPGQKRQELQNLAESYARTV
                     IEEGWPLMEQGRSSSRAEELSGELRRGVMSFEPETDAEQAIYTQSLTLIQDFDEYRSL
                     RLLEVREGIPSILWIVLIVGGVTTVGFTYLFGMKTPRLHLLMVAAFTMVLVLVLVTIR
                     SLEYPFDGIVQVRPDAFEAFLDQIHSDKKR"
     gene            2041..3006
                     /locus_tag="VHM69_09645"
     CDS             2041..3006
                     /locus_tag="VHM69_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEX2740700.1"
                     /translation="MEYRRLGGSGVKVSEISLGSWLTYGGSVAEEQATACINRAYEIG
                     INFFDTANVYMRGKAEEIVGRALKDFERDSYFLATKVYFPMGDGPNDRGLSRKHITEQ
                     CHASLKRLGVDYVDLYQCHRYDENTPLEETLRTLDDLVRQGKVLYIGVSEWTGDQISD
                     ALRIAKEMNLDRLVSNQPQYNMIQRKIEGEVIPLCEREGIGQVVFSPLAQGVLTGKYR
                     PGDAPEEGTRAADPESNVFMQQLMNEEVLSAVDELRSVASDTGLSMPQLALAWVLRQE
                     NVSSAIIGASRPEQVDDNAGASGVKLSADVLSEIDRILDGVIQRG"
BASE COUNT          582 a          850 c          943 g          651 t
ORIGIN      
        1 ccgcaacatt tatgtgcatt ttgcagatat cttcaaaaag attctaaatt cgctgttcca
       61 aaccctttac aaagtgtaac gagcgatgta tcatcctgtc agtcggttgg tgcgaaacta
      121 aaccggcaac aattaaagag cctggggcgc tactcctcct ttccctttcc ccacctatca
      181 cccctaagtg cgttccaggc tcttcccctt tttcaaaggc ttcaagttac gggcatcagc
      241 cgtcaggcaa gagttgagcg cgacgtgctg tcacggcttc gtcgcggtct ctgccgaaaa
      301 aagcggtccc cggcattcgc cgaggaccgt ttcctgaagc ctgtaacctg aagcctaact
      361 cgcttcctgt ccttccgcct gggtcaggta atagagagta tgcatgagct gggctgttgg
      421 gtcgatacgg tggacatcca cgtgggatgg gacgtgcagg aggacgtcgg tatagttgag
      481 gataaccctg atatctgcgt cgaccatcag gttggccacc tcctgggctg aggaggctgg
      541 ggttgcgatg atgccgatga tctcctcggc ttcggcgacg ctcttcttga actcgtcgat
      601 gtgcttgacg actacgttgc ccactgtgcg tccgatcttc tccgggttgt cgtcgaagac
      661 gtcgtggata acgaagcccc tcttggggag gatggtgctc gccgcgatgg cgctaccgag
      721 gttgcccgcg ccgaccaggg cgatgttgta ggtctgcctc agacccagga tgttcctcac
      781 cgcctcgacg aggtcgtagg cctggtagcc aaccccacgg gtacccgaga acccgatggc
      841 gttgaggtcg cggcgtacct gcacggggtt tatgccggtg aagtcgccca gctccttgct
      901 ggagaccgcg tcgcggccct cctcgatgag ctgctgcgtg gtcctgagat acttcgtcag
      961 cctgtttgtc aggccgggct gtaaggtcgc tcgcaccgtt tctccagctc cttcttcgct
     1021 gttcgactag ttgtgggagc attgtaacaa aatgggccgt tcttgcaata aaggtgctct
     1081 taaccgacaa caaatcagta agcaaggccg gttacagtgt aatgagtggg aaagcatttg
     1141 cacgcgggcc agcctgcgtg tacttttggg accgccggag aacgtgatgg agagcgaggt
     1201 cagccgtggc gacggctatc tggagtatcg tggttgtctt ggtggctgtc gcggtcgcgt
     1261 tggctggttt gatccctgcc caacgtctgg tgcctttctc ggttcgcgag tcgcacaaca
     1321 tccacaccgc ggcgatgttc ggggcgctat acgtcgtgta cggcctcatg gtcggtttct
     1381 cagcctatct cgtggcggtg cagttcgacg cggcgcagca gacggtggag aacgaggcca
     1441 acagcgtcga ggggatctac cttctcgccg agcagttccc cggtcagaag cggcaggaat
     1501 tacagaacct cgccgagtcg tacgcccgga ctgtgattga ggagggttgg cccctgatgg
     1561 agcagggccg gtcgagttcg cgcgcggagg agctctcagg cgagctgcgt cgcggcgtaa
     1621 tgagcttcga gccagagacg gacgccgagc aggcgatcta cacccagtct ctcacgctca
     1681 ttcaggattt cgacgagtac aggtcgcttc gtttgctcga ggtcagggag ggcataccct
     1741 ctatcctgtg gatcgtgctg atcgtcgggg gcgttaccac ggtaggcttc acctacctct
     1801 tcgggatgaa gacgccccgt ctgcacttgc ttatggtcgc cgcgttcacc atggtcctcg
     1861 tcctcgtgct agtcaccatc cgctctctcg agtatccctt cgacggaatc gtgcaggtga
     1921 gaccggatgc gttcgaggcg ttcctcgacc agattcattc tgacaaaaag cgttagaggc
     1981 gggtggctga cccgcagcgt gtactattag cacttcgtcc tctaaacaag ggaggtcctg
     2041 gtggagtaca ggcggttggg tggtagcggc gtaaaggtca gtgagatctc tctggggagc
     2101 tggctgacgt acggggggag cgtcgcagag gagcaggcga cggcctgcat caacagggct
     2161 tatgagatcg gcatcaactt cttcgacacg gccaacgtgt acatgcgtgg aaaggccgag
     2221 gagatcgtgg gccgggcgct caaagacttc gagcgggact cgtacttcct ggcgaccaag
     2281 gtgtacttcc cgatgggcga tggaccgaac gacaggggcc tctcgcgcaa gcatataacc
     2341 gaacagtgcc acgcttcgct gaagaggctc ggtgtggact acgtcgacct ctaccagtgc
     2401 catcgctacg acgagaacac gcccctggaa gagacgctca ggacgctcga tgacctggtg
     2461 cgtcagggga aggtcttgta catcggcgtc tccgagtgga ccggggacca gatctccgac
     2521 gccctcagga tagcgaagga gatgaacctg gatcgtctcg tctccaacca gccgcagtac
     2581 aacatgatcc aacgcaaaat agagggtgaa gtcataccct tgtgcgagcg cgaaggtata
     2641 ggccaggtgg tcttctcccc gctagcccag ggcgtactga cgggtaagta tcgtcctggt
     2701 gacgcccccg aggagggcac gcgtgccgcc gaccctgaat cgaacgtgtt catgcagcag
     2761 ctcatgaacg aggaggtgct ctcggccgtg gacgagctac gttcggttgc ctctgacacc
     2821 ggcctgagca tgccgcagct cgccctcgct tgggtgctgc gccaggagaa cgtgagcagc
     2881 gccataatcg gtgcttccag gcccgagcag gtcgatgaca acgccggagc ctcgggggtg
     2941 aagctctccg cggacgttct gtcggagata gacaggatcc tggacggtgt gatacagcgc
     3001 ggatagagtt cgtccacgat ttcgct
//
LOCUS       DATAIR010000395         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_394641, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000395 DATAIR010000000
VERSION     DATAIR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394641"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1446
                     /locus_tag="VHM69_09650"
     CDS             94..1446
                     /locus_tag="VHM69_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740701.1"
                     /translation="MAAASGAGEARSTGEAIRALAAGCVGNFVEWYDFAIYSYSITII
                     ATLFFPEGNRTAAIIAAFALYGVAFLARPVGGVFWGSLGDRIGRRTTLAVIVLIMGGA
                     TMLIGLLPTYGTIGVLAPILLAVLRLIQGFSGGGEFTGSTSFISEYAPDEKRGLFASI
                     SATFTTLPSLLGGLTVFAVAAGLGEEVYASWGWRIPFLVGGPLALVGLFIRLRMDETP
                     AFRELKEREEQVESTPAREAIREHGRSILLVFAIASLSALGAYTLGTYFPTYLQEVVG
                     LSRTTAIAANFLAFFVTVPLVPLVGWLGDRIGRKPLLLIGAPGFILLSVPGYMLASTG
                     NFFLAILGQLLVALPWSFVVSAVVVIIVEIFPTRVRYSGASIGYNLGYMIFGGSAPIV
                     ATALVSATGSDIAPAIYLIVVAVLVLPAIFLLPETRRLSPLRDADKARPSGSTEPGVT
                     "
     gene            1480..2436
                     /gene="murQ"
                     /locus_tag="VHM69_09655"
     CDS             1480..2436
                     /gene="murQ"
                     /locus_tag="VHM69_09655"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX2740702.1"
                     /translation="MPPCLKIEEVTQQMDELPTTELRNPASHRLDDMPVEDVLRLMNQ
                     EDRKVPGAVEKALPRIAEAVELLVEAWKQGGRWIYVGAGTSGRVAALDAAECPPTFGV
                     PSDRVLALLAGGQTAATQAIEGAEDDRSAAVRDLESLSLDPGDVVVGLAASGRTPYVV
                     AAVRHASEKGCATVGISNNPGSELGAAASVGIELDTGPEVLTGSTRLKAGTSEKLVLN
                     MLSTASFTRLGKVYENFMVDLQATNEKLKDRSLRIVKEAVGVPDEEARELLHSAGGSV
                     KVAAVMGKARVPANEARGLLDAAGGNVRRALEAARSAHPAER"
     gene            2433..>2747
                     /locus_tag="VHM69_09660"
     CDS             2433..>2747
                     /locus_tag="VHM69_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740703.1"
                     /translation="MKPHALVGDVLAPEPLGRTAVIIEDGKISDVLRSPRQAELPHER
                     REVSGFICPGFIDLQINGAFGIDVAPDPEALKALARKLPRTGTTSFLPTLISSPLEVY
                     ADF"
BASE COUNT          480 a          855 c          893 g          519 t
ORIGIN      
        1 atatgttgtc tataccaata agctgggtgg aggaatcaga ggttgagcga gcgggtggat
       61 ggaacgatgt cacactggaa ggagaaatag ggaatggcgg cggcatctgg agcgggagag
      121 gcacggagca ctggggaggc cataagggcg ttagcggcgg ggtgcgtggg taatttcgtc
      181 gagtggtacg actttgcgat ctactcgtac tcgatcacga taattgcgac gctcttcttc
      241 cctgagggga accgcaccgc cgccataatc gcggcgttcg cgctctatgg cgtggctttc
      301 ctggcgcgtc ctgttggcgg tgttttttgg gggagtttgg gggacaggat cggacggaga
      361 accactctcg ccgtgatcgt cctcatcatg ggtggggcga cgatgctcat tggcctgttg
      421 ccgacatacg gcaccatagg cgtccttgct ccgatactgc tcgcggtgtt gcgtctgatc
      481 cagggtttct cgggcggcgg cgagttcaca ggctcgacct cgttcatctc cgagtacgcc
      541 cctgacgaga agcggggcct cttcgcatcc atctcagcca cgttcaccac cctgccctcg
      601 ctccttggcg ggttgacggt cttcgcggtc gctgccggcc tcggggaaga agtctatgct
      661 tcctggggat ggcgcatacc gttcctggtc gggggaccgc tcgccctcgt tgggctgttc
      721 atcagactaa ggatggacga gacgccagcc ttcagggagc tcaaagagcg cgaggagcag
      781 gtggagagca ccccggctcg ggaggcgata cgggagcatg gcaggagcat actgctcgtc
      841 ttcgccatcg cctcgttgag cgcgctcggg gcgtacaccc tgggtactta cttccccacc
      901 tatctgcagg aagtagttgg cctgagcagg accacagcca tcgccgccaa cttcctcgcc
      961 ttcttcgtca ccgtgccgct ggtgccgctc gtcggctggc tcggggacag gatcggtcgc
     1021 aagccactgc tcctgatcgg agcgccgggc ttcatacttc tgagcgtgcc aggctacatg
     1081 ctggcttcca caggcaactt cttcctcgcc atcctggggc agctactcgt ggccttgcca
     1141 tggagcttcg tcgtctccgc ggtcgtcgtg ataatcgtcg agatcttccc taccagggtt
     1201 cgctactcgg gcgcctccat cggctacaac ctcggataca tgatcttcgg tgggtcagcc
     1261 ccgatagtag ctacggccct ggtttcggcg acgggaagcg acatcgcgcc tgcgatctac
     1321 ctaatcgtcg tcgccgttct cgtactgcct gctatcttcc tgctgcccga gaccaggcgg
     1381 ctctccccgc tccgtgacgc cgataaggcc aggccgtcgg gatccacgga gcctggggta
     1441 acctgatagc caacggctcg ccgaaggggg tgccttctgg tgcccccttg ccttaagatc
     1501 gaggaggtca cgcagcagat ggacgagctt cctacgaccg agctacgaaa cccggccagc
     1561 catcggctcg acgatatgcc tgtggaggac gtactgcgtc tcatgaacca agaggaccgc
     1621 aaggtgcctg gtgccgtcga aaaggccttg cccaggatcg cggaggccgt ggagctgctc
     1681 gttgaggcct ggaagcaggg gggacgctgg atctacgttg gcgcggggac cagcgggcgc
     1741 gtcgccgccc tcgacgcggc cgaatgccct ccaacctttg gggtcccgtc ggaccgggtg
     1801 ctcgcgctgc tcgcgggcgg acagaccgcc gcgacgcagg ccatagaagg ggccgaggac
     1861 gaccggtccg ccgccgtccg cgacctcgag agcttgagcc tggatccggg cgacgttgtg
     1921 gtagggctcg cagcgagcgg acgtacgcct tacgtcgtgg ccgctgtcag gcacgcctcc
     1981 gagaaaggat gcgccactgt tggtataagc aacaaccccg gtagcgaact gggtgctgcg
     2041 gctagcgtag gcatcgagct ggacaccgga ccggaggtct tgaccggttc gaccaggctc
     2101 aaggcgggca cttcggagaa gctggtcctc aacatgctct ccaccgcatc tttcacacga
     2161 ctgggcaagg tatacgagaa cttcatggtg gatctccagg ccacaaacga gaagctaaaa
     2221 gatcgctccc tgcgcatcgt caaggaggca gtgggcgtcc ccgacgaaga agccagggag
     2281 ttgcttcact ccgccggcgg ctccgtcaag gtggccgcgg tgatgggtaa ggcgagggtg
     2341 ccggcaaacg aagcccgcgg gctactagat gcggccgggg ggaacgtgcg ccgggcgctc
     2401 gaagcggcgc gttcggccca tccggcggag agatgaagcc acacgccctc gtcggcgacg
     2461 tcctggcccc cgagccgtta ggccggacgg ccgtcatcat agaggacggc aagatctccg
     2521 acgtactgcg ctctccacgc caggccgaac taccccacga gcgccgcgag gtctccggtt
     2581 tcatctgtcc cggtttcatc gacctacaga tcaacggcgc cttcggaata gacgtagctc
     2641 cggacccgga agccctgaaa gcactggccc gcaagctgcc gcggacgggc accacctcgt
     2701 tcctgcccac cctgatctcc tcccctctgg aggtttacgc cgacttt
//
LOCUS       DATAIR010000396         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_302551, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000396 DATAIR010000000
VERSION     DATAIR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302551"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1078)
                     /locus_tag="VHM69_09665"
     CDS             complement(170..1078)
                     /locus_tag="VHM69_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740704.1"
                     /translation="MAEAQLQQDDAATTSGVETRSRRQDFTRVFFSNRLAVFGTVVIS
                     IFILMAIFAPLIAPYDPLQQDLVAKFAPPSAAHPFGQDELGRDILSRVIYGARISLSA
                     GLAAVAIATAAGTLIGLVAGYFGNWSDSVLMRLMDVLLAFPSILLAIVIVSILGPSLA
                     NAMLAIGVVFIPQIARVVRSAVISVRERDYIEAERALGAGNTQIIFSGVLPNSMAPLI
                     VQATLILATAILDVAALSFLGLGARPPSPEWGAMLTDAFRAGYGVFTVGQHAIIFPGA
                     AIALSVLAVNFIGDGLRDALDPRRIR"
     gene            complement(1082..2083)
                     /locus_tag="VHM69_09670"
     CDS             complement(1082..2083)
                     /locus_tag="VHM69_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740705.1"
                     /translation="MVEFVGRRIFHIIPVLLGVSIVVFFMVRAIPGDPAQILLGQQAT
                     EDQVAQVRENMGLDKPIFVQYGLFLVDAVRGDLGDSIVTGRPVTTELAERFPATLELT
                     AFAMIIAVLVGVPVGVISAVRQYSILDKVTSVLALTGLSMPIFWLAMILIVIFFVNLG
                     WLPFPGRVSTDVSIPAITGLVLVDSLLALNFAAFWDGLLHLIMPAVALGTIPMAVVTR
                     MTRSSMLEVMNEDYVRTARAKGVVPWRVVFKHALRNAMLPTVTVIGLQVGLLMGGALI
                     TETIFSWPGVGQIAYESIFRRDYAMIQGVVLYGATLFVLVNLLVDILYAVLDPRVRY"
     gene            complement(2130..>2922)
                     /locus_tag="VHM69_09675"
     CDS             complement(2130..>2922)
                     /locus_tag="VHM69_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2740706.1"
                     /translation="PVEDAGLKVEFRPPLTIGYLAMNNQQKPFDDVNVRKAIYQAIDM
                     EKIVKAFFGSTGQLATTYMPPTVPFWNSSIERFPFDPEKAKQMLKDAGQENLETELWY
                     MPIPRPYMPDGKGIAQAMQADLEKVGVTAKLVTYEWGTYLEKTGEGAHPMALLGWTGD
                     NGDPDNFLNTLLGSASATPTDALNIAYYKNPEADKLLLQAQTTIDEAERERLYMKAQE
                     LLTQDAPWVPIAYAKPPLGFQKDVSGYQASPTGGEAFNTVSLGGK"
BASE COUNT          562 a          896 c          955 g          509 t
ORIGIN      
        1 caagcaggct gcggttaggc cgccacatgg tcctgtagct cggcgtgctt ttgcgttctc
       61 gggtccacga cccgtatggt ttggttctca cggtggacta tgatacatcc cccgcgcaac
      121 aaaacaacac gaagcgttaa caatctttcg cgaggacgac actaggaagc tacctaatgc
      181 ggcggggatc gagggcgtcg cgcaggccgt cgccgataaa gttgacggcg aggaccgaga
      241 gggctatggc ggcacctggg aagattatgg cgtgctgtcc caccgtgaat acaccgtaac
      301 cggcccggaa agcgtcggtc agcatggcgc cccactcggg ggagggaggg cgggcgccga
      361 gcccgaggaa cgagagggcg gcgacgtcga ggatggccgt agcaaggatg agggtagcct
      421 gaacgatgag cggggccata ctattcggca gcactccgga gaatatgatc tgggtgtttc
      481 ccgcgccgag agcccgctcc gcctcgatgt agtcccgctc cctgacggag atgaccgcgg
      541 agcgcacaac ccgcgcaatc tgcgggatga acacgacgcc gatggcgagc atggcgtttg
      601 ccaggctcgg cccgaggatt gaaacgatga cgatagcgag caggatgctc gggaaggcga
      661 gcaagacgtc catcagccgc atcagcaccg agtctgacca gttgccgaag tacccggcca
      721 cgagccctat cagggtgcct gccgcggtgg cgatggctac ggctgcgaga cctgccgaga
      781 gcgagatcct cgcgccgtat atcacacgcg agaggatgtc ccggcccagt tcgtcctgcc
      841 cgaacggatg cgcggcgctc ggcggcgcga acttggcgac gaggtcctgc tgcaacgggt
      901 cgtagggggc gatcaggggc gcgaagatcg ccatcaggat aaagatgctt atgacgaccg
      961 taccgaagac ggccaggcgg ttggagaaga agacgcgcgt gaagtcctga cggcggctgc
     1021 gcgtctcgac gcccgaagtg gtcgcagcgt cgtcctgctg aagttgcgcc tcggccatcg
     1081 tctaatacct caccctcggg tcgagtaccg catagaggat atccacgagc agattcacca
     1141 gtacgaagag cgttgccccg tagaggacca cgccctggat catggcgtag tccctgcgga
     1201 agatcgactc ataggcgatc tgcccaacac cgggccagga gaagatggtc tccgtgataa
     1261 gggcaccgcc cattagtagt ccgacctgga gccctatcac cgtcaccgtc ggcagcatgg
     1321 cgttcctcag ggcgtgcttg aagacgaccc gccaggggac gacgcccttg gccctggccg
     1381 tccgcacgta gtcctcgttc atcacctcga gcatgctgga gcgggtcatg cgcgtcacca
     1441 cggccatggg gatggtcccg agggcgaccg ccggcatgat gagatgcagc agcccatccc
     1501 agaatgccgc gaagtttagc gccaggagcg agtcgaccag cacaaggccg gttatggccg
     1561 ggatggagac gtcggtgctc acgcggccag ggaaaggcaa ccatcccagg ttcacgaaga
     1621 agatcacaat aaggatcatc gcgagccaga agatgggcat gctcagcccc gtgagcgcga
     1681 ggaccgaggt gaccttgtcg agtatcgagt actgtctgac cgcggagatg accccgacgg
     1741 gcaccccgac gagcaccgcg atgatcatgg cgaaggccgt gagctccagc gtggcgggga
     1801 agcgctcggc cagctccgtc gtaacgggac ggcccgtcac tatggagtca ccgaggtccc
     1861 ccctcacggc gtcgacgagg aagagcccgt actgcacgaa gatgggcttg tccaggccca
     1921 tgttctccct gacctgggcg acctgatcct cggttgcctg ctggccaagg agtatctgag
     1981 ccgggtcgcc ggggatagcc cgcaccatga agaacaccac gatagacacc ccgagcagta
     2041 cggggatgat atgaaatatc ctgcgcccga cgaactctac cagcgcccga acctcctacg
     2101 cggagcctcc gaggaggccc cgctccaact tacttgccgc ccagactgac ggtgttgaac
     2161 gcctcgccgc cagttgggct cgcctggtaa ccgctcacgt ccttctggaa gcccagggga
     2221 ggcttggcgt aggcgatggg cacccacggg gcatcctgcg tcagtagctc ctgtgccttc
     2281 atgtagagcc gctcgcgttc ggcctcatcg atggtcgtct gggcctgcag cagcagtttg
     2341 tccgcttcgg ggttcttgta gtaggcgatg ttgagcgcgt ccgtgggcgt ggcgctcgcg
     2401 ctgcccagga gcgtattgag gaagttgtca gggtcaccgt tgtccccggt ccacccgagc
     2461 agggccatcg ggtgagcccc ctcgccggtc ttctcgaggt aggtgcccca ctcgtaggtg
     2521 acgagcttgg ccgtcacgcc gaccttctcc aggtcggcct gcatcgcctg ggctatgccc
     2581 ttgccgtctg gcatgtaagg acgcgggatc ggcatgtacc acagctcggt ctccaggttc
     2641 tcctgtcctg cgtccttgag catctgcttg gccttctcgg gatcgaacgg gaaacgctcg
     2701 atactggaat tccagaacgg gacggtgggc ggcatgtagg tcgtggcgag ctggcccgtg
     2761 ctcccgaaga aggcctttac gatcttctcc atgtctatgg cctggtagat agccttccgc
     2821 acgttgacgt cgtcgaacgg cttctgctga ttgttcatcg ccagataacc gatcgtgagg
     2881 ggcggcctga actcgacctt gagccccgcg tcctcgacgg gc
//
LOCUS       DATAIR010000397         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_57721, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000397 DATAIR010000000
VERSION     DATAIR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57721"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VHM69_09680"
     CDS             complement(<1..743)
                     /locus_tag="VHM69_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX2740707.1"
                     /translation="MERDDEQTMRRARMVLDFNWTGEYTQPGPRLYPHQWSWDSALIA
                     IAYAHYDQNRAEKELRHLFEAQWKNGLVPQIVFNSTFSDYFPGPGFWHAKESPAAPEH
                     IKTSGVVLPPVHATAVLRLHEHAEDKDRARIFLQYAFPRLAAWHEYLYRERDPREEGL
                     VYIRHPWESGMDNSPIWDVVLQRLQLRPEVIPRYHRADTHTVSTDDRPTSDDYDRFAY
                     LVKFFADRDYDERRIREECPFLVQDVLFNS"
     regulatory      1002..1112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1178..3031
                     /locus_tag="VHM69_09685"
     CDS             1178..3031
                     /locus_tag="VHM69_09685"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX2740708.1"
                     /translation="MSANLRELLDTRVLIGDGAVGTLLGERGVGFGHPYARANPSHPE
                     MVYAIHEEYLRAGANVIETNTFAANCFKLETHDLEERVREVNAEGARLARKAALDIAG
                     AGDRALVLGAIGPLGRSLTPVGPVSPEQARSVFREQAEALLEGGADVILLETFPDLVE
                     LRLAYVAVKDLGAPVLAYKTFVEDGETLAEGLPERAAREISSWGADLTGANCTVGPQR
                     MVEIIEQMAAGAGPVAAFPNPGLPQLVDGHIRFSRDVDHFAEYGVKLAQAGARLVGGC
                     CGTTPAHVKALAEALRDFRVDGTEVRRRVAVVEQAERREVSSEPVSEFAERLRTGFAV
                     TVEVDLPRGNAITQVVEAAQRLKERGIHAIDISDGARARLRMHPVAAAKIVQDEAGIE
                     VVSHISCRDRNIIGLQADLLGAAALGVKNILAVTGDPAQIGDYPEATSVFDTDSVGLV
                     HVLSKMNQGEDLAGNPIGDPPGFLIGASFNPTAEDLDEEVEKLRRKVEAGAHAFWTQP
                     VFEIDALENALERIGDDGVCILLGLMPLRSARQAEFLHHEVPGINIPKHIRETLATLS
                     AEDAPKYGVEVAQKLLVEAHPLVGGAYIMPPASAPDLAADVVEAISLPTWG"
     gene            3033..3560
                     /locus_tag="VHM69_09690"
     CDS             3033..3560
                     /locus_tag="VHM69_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HEX2740709.1"
                     /translation="MILFINGPFGVGKTSVSKLLDEKIPDAMLYDPEVIGAVLKRVLG
                     PFKKVEDYQDYDLWRTLVVGGVRVLRRASARTLIIPMTVWRRDLFDPIITGLRRIDGD
                     LACFRLTASRDVLMDRISSDTEDREARAWRTSHADICLEALRDPAFGTEVRTDDRAPG
                     DVADRILQIAGMPTG"
     gene            3572..>3988
                     /locus_tag="VHM69_09695"
     CDS             3572..>3988
                     /locus_tag="VHM69_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2740710.1"
                     /translation="MVLQRTKDEERQARLVEFRELLKQRIVLLDCAMGTMIQSYELSE
                     EDYRGERFADHPGELKGNNDLLSITQPQIIRDIHEAAMEAGSDIIETNTFSSTSVAQA
                     DYKMEDLAYELNYEAARIAREAADDFATPERPRFVAG"
BASE COUNT          768 a         1254 c         1284 g          682 t
ORIGIN      
        1 gagttgaaga gcacgtcctg caccagaaac gggcactcct cacggatgcg tcgctcgtcg
       61 tagtcgcggt cggcgaagaa cttgacgagg taggcgaaac gatcgtagtc gtcgctggtc
      121 ggacggtcgt cggtagagac ggtgtgggtg tcggcccggt gataccgtgg gatcacctcc
      181 ggcctgagtt ggagccgctg gaggacgaca tcccatatcg gcgagttgtc catcccgctc
      241 tcccacgggt gccggatata caccagcccc tcttcgcgcg ggtcgcgctc gcggtagagg
      301 tactcgtgcc aggccgccag cctggggaag gcgtactgca ggaatatcct cgccctatct
      361 ttgtcttccg catgttcgtg gagacgcagt accgccgtgg cgtggacagg cggcaggacc
      421 acgcccgagg tcttgatgtg ttctggagcg gcggggctct ccttcgcatg ccagaatcca
      481 ggacccggga aatagtcgga gaacgtggag ttaaagacga tctggggcac cagcccattc
      541 ttccactgcg cttcgaagag atgccgcaac tccttctcag ccctgttctg atcgtagtgg
      601 gcgtaagcga tggcgattag cgcggagtcc cacgaccact ggtgcggata caatctgggt
      661 cccggttggg tgtattcgcc cgtccagttg aagtccagga ccatcctggc ccgccgcatc
      721 gtttgctcgt cgtctcgctc cattaattgg tcctcctgga gagctgtatc agaagtctac
      781 gcgggccggt agtcgtccgc ttctatagcg ggcccacggg aaaccacgag gcatgtctcc
      841 ccgacacact accccgttgc ccgcgaagcg catcaggctg ccatccaaca cgcagatgat
      901 cccgcatatt acaaccgtcc cggtggaggg ccgtgagatt gaaatcctgt gcctatcgtg
      961 ctgtaattgc ctggtcatcg gtcgcaaaga gcaggccatg acgctcatcg agagcggtgg
     1021 agggacgggc cctgagaagc cgcggcaacc ggcgaggcgc gagcctcgtg acaggtgcca
     1081 actcccgcgg agcagactcc ggaagatgag gtgaaagagc agacgcagcc tctctcctcc
     1141 gcgcgaactt gaaacgaata aggagagagg tcctatcttg agtgccaacc tcagagagtt
     1201 actcgatacg agggtcctga tcggggacgg cgccgttggc acgttgcttg gcgagcgcgg
     1261 cgtcggattc ggccatccct acgcccgtgc gaacccctct caccccgaga tggtctacgc
     1321 aatccacgag gagtacctgc gggcgggcgc gaacgtcatc gagacgaaca ccttcgccgc
     1381 caactgtttc aaactggaga cccacgacct ggaagagagg gtgcgagagg tcaacgcaga
     1441 gggcgcccgc ctcgcccgca aggccgccct tgacatcgcc ggcgcaggag acagggcact
     1501 cgtactcggg gccatcggcc cgctcggacg ttcgctcacg cctgtcggtc ctgtgagccc
     1561 cgagcaggcg aggtcggtct tccgcgagca ggccgaggcg ctcctcgaag gtggggccga
     1621 tgtgatcctg ctcgaaacct tccccgacct cgtcgaattg cggctcgctt acgtggcggt
     1681 caaggacctc ggcgcgccgg tgcttgcgta caagaccttc gtcgaggacg gcgagacgct
     1741 cgcagagggg ctcccggagc gcgctgcgcg tgagatctcc tcctggggcg ccgacctgac
     1801 gggggcgaac tgcaccgtag gcccgcagcg gatggtcgag ataatcgagc agatggccgc
     1861 cggagcaggt cccgtcgccg ccttcccgaa ccccggtctg ccgcagctgg tggacggcca
     1921 cattcgcttc agccgcgacg tggaccactt cgccgagtac ggcgtgaagc tcgcccaggc
     1981 aggagcgcgc ctcgtcggag gctgctgcgg gaccaccccc gcccacgtga aggcgctcgc
     2041 tgaggcactg cgggacttca gggtcgacgg tacagaggtg cggcgccgcg tggccgtcgt
     2101 ggaacaggcc gagcggcggg aggtgtcgtc cgagcctgtt tcggagttcg cggagcgcct
     2161 gcgcaccggc ttcgccgtca ctgtcgaggt ggacctgccg cgcggcaacg ccatcacaca
     2221 ggtcgtcgag gccgcgcagc gtctgaagga acgagggatc cacgccatag acatctcaga
     2281 cggggcgcgg gcgaggctca ggatgcaccc cgtggctgcg gccaagatcg tgcaggacga
     2341 ggccggcatc gaggtcgtct cccacatctc atgccgtgat cggaacatca tcggcttgca
     2401 agccgacctg ctcggcgcgg cggcactagg tgtaaagaac atccttgccg tgacggggga
     2461 cccggcccag atcggggact acccggaagc tacctcggtc ttcgacaccg actctgtcgg
     2521 cctcgtgcac gtcctctcga agatgaacca gggagaggac ctcgcgggca accccattgg
     2581 tgatccgcca ggtttcctta tcggggcgtc tttcaacccg acggccgaag acctcgacga
     2641 ggaggtcgag aagctgcggc gcaaggtcga agccggcgcg cacgccttct ggacgcagcc
     2701 tgtattcgag atcgacgccc tcgagaacgc cctcgaacgt atcggcgacg atggggtgtg
     2761 catcctgctc ggcctcatgc ccctgcgcag cgcccgccag gccgagtttc tccaccacga
     2821 ggtcccaggc ataaacatcc cgaagcacat ccgcgagacg ctggcgacgc tctcagcgga
     2881 agacgccccg aagtacggcg tcgaggtagc ccagaagctc ctcgtcgaag ctcatccgct
     2941 cgtcggcggc gcgtacataa tgccacccgc gagcgccccc gacctcgccg cggacgtggt
     3001 cgaggccatc agccttccga cctggggata aggtgatcct gttcatcaac ggtcctttcg
     3061 gcgtggggaa gacgagcgtc tcgaagcttc tcgacgagaa gatcccagac gccatgctct
     3121 acgacccaga ggtaatcggc gcagtcctca agagagttct cggaccattc aagaaggtcg
     3181 aagattatca ggactacgat ctctggcgca ctctggtcgt cggaggcgtg cgtgtactga
     3241 ggagagcgtc ggcgcggacg ctaatcattc ccatgacggt gtggaggcgg gacctcttcg
     3301 accccatcat caccgggctc cgcaggatag atggggatct cgcgtgtttc cggcttaccg
     3361 catccaggga tgtgctgatg gatcgcatct cgtccgatac cgaggatagg gaagcccgtg
     3421 cgtggcgcac gtctcacgcc gatatctgcc tggaggctct gcgcgacccg gccttcggaa
     3481 cggaggtacg aacggacgac cgcgcgccag gcgatgttgc tgacaggatc ttacaaatcg
     3541 cgggaatgcc gaccggctag aataggagtt catggttctc cagcgcacga aggacgaaga
     3601 gagacaggcc aggctagtcg agttccggga actgttgaag cagcgcatcg tgctgctcga
     3661 ctgtgcgatg gggacgatga tccaatccta cgaactctct gaggaggact acaggggaga
     3721 acgcttcgct gaccatccag gagagctcaa gggcaacaac gacctgctgt cgatcaccca
     3781 gccacagata atccgcgaca tccacgaggc cgctatggag gccggctctg acatcatcga
     3841 gacgaacacc ttcagttcga cctccgtcgc ccaggcagat tacaagatgg aggaccttgc
     3901 ctacgaattg aactacgaag ccgcccgcat agcccgcgag gctgcggacg acttcgcgac
     3961 gccagagaga ccgcgcttcg ttgcaggc
//
LOCUS       DATAIR010000398         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_394738, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000398 DATAIR010000000
VERSION     DATAIR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394738"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1972
                     /locus_tag="VHM69_09700"
     CDS             179..1972
                     /locus_tag="VHM69_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX2740711.1"
                     /translation="MRNDSGGTPSGYSSSSLNPFNSGKHASRRRGIAGPIIIICAVIA
                     VLVAADFWLNTGKIHRGVEVGSVSLGGKTPSEARQVVRDQAMGPLKEIEFNGPEHFTR
                     RASELGVDFNIDETVDKAYAVGREGNLLDRLSERLKASFGGATIPPDIDYQPAKARAE
                     VREIAGQIDHQPREANLKVYGSEVEVTRSRDGYHLNTAATMASLDSAIDDMSGKASLV
                     GDVLDPDVTTAEAETAAEKARGALSEQLVLKAVGKSWTVSPADLGSTLDVTKQDGKID
                     VSLNRDRLDGVLTNVYNDLTVEPVEASYDFASNGDVIVKPSHEGRTVEGDKLLDAIQG
                     GLFEGQREYQVQTTVDKPRYTTAGLEAKKPSTLLGTYKTNYTATTDQGAERVANLEMA
                     SNAVSGTFVAPGDTFSMVQHVQDLSYKEAHVIVNSKEKVAEGGGLCQVTSTLYNAVLY
                     AGMEVTERTPHDSQLPYIRPGMDATVWYGDTSTTVDDTDMKFTNTSDGYVLLQEYVSD
                     DGYIYANVYGVPNNINVEMSSEKVWMTEDASEWITYYTRSKGGKVVYRDSWKSQYSAL
                     YDDKGKKIKTPKVPIAEVDGSYYGIDFSALE"
     gene            complement(2052..3377)
                     /locus_tag="VHM69_09705"
     CDS             complement(2052..3377)
                     /locus_tag="VHM69_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX2740712.1"
                     /translation="MLTQTDKAHDLAERFGGESEGEFAPASIPVSRIAESYGTPFYLY
                     HGEMITDRVRRVRESLGTEVSYSVKANPSLGVCQLIAGEREAGAEVASSGELAVARAA
                     GFEPEDIVFAGPGKTNDELSRAVDEGIFAVNVESLNEIDRLAHIAGQAGKSIGVGLRI
                     NPAAQLMGSGMRMGGTVGQFGMDQAELDEAVQKTLSHPELVLRGVHVYTATQVFEVEP
                     LLEHCSNILEIALEAADLSGSPMEMIDFGGGFGVPYFEKMTEFDLDSFADGFRELLSS
                     YRSDPRLEGCRFLFELGRYLVADAGVYVTRAVDVKETRGKTFVVTDGGMNHHLTATGN
                     MGQVFRKAYPLLNLTRMSGVPEEGVAVAGPCCTPLDTFGNNIPLATPEAGDLIGVFYS
                     GAYGYSASNLGFLSHPSPAEVLLWRGEAHLLRPGGRPEDVLEGQRALAS"
     gene            complement(3358..>3540)
                     /locus_tag="VHM69_09710"
     CDS             complement(3358..>3540)
                     /locus_tag="VHM69_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740713.1"
                     /translation="VIAVISLKDGDVAVDSIREYAIKNGPPYMIPKEILVWDELPKTG
                     SGKIDRKGISSAYANG"
BASE COUNT          713 a         1096 c         1133 g          598 t
ORIGIN      
        1 ctaaaaggtg gcgtactcaa ccgcgccgtg ttaggctttc cgggcggcgc tcgctcgggc
       61 cagggtgaga ggtcgccagc taaagcgaga gggggggacc tctccacggt aatctgttgt
      121 tcgggcgcgt ggaaggccgc acgcggaggg tagatagttg agtctcaaaa acaggaaaat
      181 gagaaacgat agcgggggga cgccttccgg ctatagctct tccagcctga acccgttcaa
      241 ctcaggcaaa cacgctagcc ggcgtcgtgg gatcgctggc ccgataatca ttatctgcgc
      301 agttatagcc gtgcttgtgg cggcggactt ctggctgaac acgggcaaga tacaccgcgg
      361 cgtcgaggtc ggcagcgtct ctttgggcgg caagacacca tccgaggcgc gccaggtcgt
      421 cagggaccag gccatgggac cgctcaagga gatagagttc aacggcccag agcacttcac
      481 ccgcagggcc agtgagctcg gcgttgactt caacatagac gagaccgtgg ataaagccta
      541 cgccgtcggg cgcgagggca acctcctcga ccgcctctca gagcgtctga aagcatcctt
      601 cggcggggcg acgatacccc ctgatatcga ctaccagcct gcgaaggcgc gggccgaggt
      661 gcgtgagatc gcgggccaga tagaccacca gccgcgggag gccaacttga aggtctacgg
      721 ctccgaggtc gaggtcacga ggtctcgcga cggctaccat ctgaacaccg cggccaccat
      781 ggcgagcttg gatagcgcaa tcgacgacat gagtggcaag gccagtctgg tcggcgacgt
      841 actcgacccc gatgtcacga cggccgaggc cgagacggcg gcggagaagg caagaggagc
      901 cctctccgaa caactcgtgc tcaaagccgt gggcaaaagc tggacggtct cgcctgcgga
      961 tctgggttca acactcgacg ttaccaagca ggacggcaag atcgacgtca gcctcaaccg
     1021 agaccgtctg gacggcgtcc tcactaacgt ctacaacgac cttacggtgg agcccgtcga
     1081 ggcgagctac gacttcgcct ctaacggcga cgtcatcgtc aagcccagcc atgaagggcg
     1141 gaccgtcgag ggagacaagc tcctcgacgc catccagggc ggcctcttcg aaggccagcg
     1201 cgagtaccag gtgcagacga ccgtcgataa gcccaggtac accaccgcag ggctcgaggc
     1261 caagaagccg agcacgctgc tcggcaccta caagaccaac tacactgcga cgaccgacca
     1321 gggggccgag cgcgtggcga acctggagat ggcgtcgaac gcagtgagcg gcacgttcgt
     1381 ggcgccggga gataccttct ccatggtcca gcacgtccaa gacctgagct acaaggaagc
     1441 gcacgtcatc gtgaacagca aagagaaggt cgctgagggc ggcgggctct gccaggtcac
     1501 ctctacgctc tacaacgccg tgctctacgc cggcatggag gtaacggagc gaacgccgca
     1561 cgactcgcag ttgccttaca tcaggcccgg gatggacgcg acggtctggt acggtgatac
     1621 ctcgaccacc gtggatgaca cggatatgaa gttcacgaac accagcgacg ggtacgtgct
     1681 cctccaggag tatgtgtccg acgacggcta catctacgct aacgtctacg gcgtccccaa
     1741 caacatcaac gtagagatgt cttcagagaa ggtctggatg accgaagacg cttccgagtg
     1801 gatcacgtac tacacgagga gcaagggcgg caaggtcgtc tacagagaca gctggaagag
     1861 ccagtacagc gccctctacg acgacaaggg gaagaagatc aaaactccaa aagtgccgat
     1921 agccgaggtg gatgggagct actacgggat agattttagc gccctcgagt agccgccgtt
     1981 aggcacgcca gccggtcagc aaaaggcccc cgggttccgg gggcctttct ttgtgtcagc
     2041 gtttttcgct gtcagctcgc gagtgctctc tgcccttcga gcacgtcctc gggcctgccg
     2101 ccgggacgca ggaggtgggc ctcgcccctc cagagaagta cctcggctgg cgaggggtgg
     2161 ctcaggaagc ccaggttgga ggccgagtag ccgtaagccc cgctgtagaa gacgccgatg
     2221 aggtccccgg cctcgggagt ggccaggggt atgttgttgc cgaacgtgtc gagcggtgtg
     2281 cagcaaggcc cggcaacggc gacgccctcc tcgggcaccc cgctcatgcg ggtcaggttg
     2341 aggagcgggt aggctttgcg gaagacctgt cccatgttcc cggtcgccgt gaggtggtgg
     2401 ttcatgccgc cgtcggtgac gacgaaagtc ttgcccctcg tctccttcac gtccaccgcc
     2461 ctcgtgacgt agacgcctgc gtcggccacg aggtagcgcc ccagctcgaa gaggaagcgg
     2521 cacccttcga ggcgagggtc cgagcggtag gaggagagca gctcgcggaa accgtcggcg
     2581 aagctgtcca ggtcgaactc cgtcatcttc tcgaagtagg gaaccccgaa gccaccgccg
     2641 aagtcgatca tctccatcgg actccccgag agatccgccg cctccaatgc tatctccagg
     2701 atgttgctgc agtgctccag aaggggctcg acctcgaaga cctgggtcgc cgtgtagacg
     2761 tggacgccgc gcaggacgag ctcggggtgg gagagcgtct tctggaccgc ttcgtccagc
     2821 tcggcctggt ccatgccgaa ctgcccgaca gttccgccca tgcgcatccc cgagcccata
     2881 agctgggctg cggggttgat cctgagtccc accccgatgc tctttcccgc ctgcccagct
     2941 atgtgtgcga ggcggtcgat ctcgttcaaa gactcgacgt tgacggcgaa gatgccctca
     3001 tcgacggcgc gactcagttc gtcgttggtc ttgccgggac ccgcgaagac gatgtcctca
     3061 ggctcgaagc cagcggcgcg tgccacggcc agttccccgg aagaggcgac ctcggctccg
     3121 gcctcgcgct cgcctgctat aagctggcac accccgaggc tggggttcgc cttcaccgag
     3181 taggagacct cagtacccag ggactcgcgg acgcggcgga cccgatcagt gatcatctcc
     3241 ccgtgataga ggtagaaggg cgttccatag ctctccgcta tgcgggagac ggggatgcta
     3301 gcgggggcga actcgccctc gctctctccg ccgaagcgct cggccaggtc gtgggcctta
     3361 tccgtttgcg taagcactgc ttatcccctt gcggtcgatc ttgcctgaac ccgtcttggg
     3421 aagttcgtcc cagacgagta tctccttcgg aatcatgtaa ggtgggccgt tctttatggc
     3481 gtactcgcgg atggagtcca ccgccacgtc tccgtccttc agcgagatca ccgcgatgac
//
LOCUS       DATAIR010000399         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_25491, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000399 DATAIR010000000
VERSION     DATAIR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25491"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="leuD"
                     /locus_tag="VHM69_09715"
     CDS             <1..563
                     /gene="leuD"
                     /locus_tag="VHM69_09715"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2740714.1"
                     /translation="KAVPLGWDDVDTDQIVPSDALKRIERTGFGQFLFSAWREDPDFI
                     LNKPEHDGAVVLIAGKNFGSGSSREHAPWAIQDYGFGAVIAESFADIFKNNCAKIGLL
                     AVELPEDAVQKLLEAIRSDPEAGVSVDLEGRVVKGPGIEEPFAFDNFARYRLLNGLDD
                     IGLTLTHEDELEEFEKTRLDYLPRVQ"
     gene            624..1046
                     /locus_tag="VHM69_09720"
     CDS             624..1046
                     /locus_tag="VHM69_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2740715.1"
                     /translation="MAKKADKALKSLKKDVAKLKKQNDKLAETLEKAREDQASAHIEL
                     RKLLEEHLTTHDVGPDESVPEADESTAVDSSRDGEGEEEPEATEEQPEATEEEPEVTE
                     AAKRRAKEFGVDLSSVKGTGSGGRILVNDVEAAAEAKQ"
     gene            complement(1077..1952)
                     /locus_tag="VHM69_09725"
     CDS             complement(1077..1952)
                     /locus_tag="VHM69_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG/SRA domain-containing protein"
                     /protein_id="HEX2740716.1"
                     /translation="MPERLFGHVPGYPEGSLFESRAELNASRVHVPTQAGISGSQTEG
                     AESIVLSGGYEDDADYGDTIIYTGHGGRDTSTGQVTHDQPFARGNRALALSKQNGLPV
                     RVIRGSNHDSPYSPPSGYSYDGLYAVEDFWHEAGKSGFEIWRFRLTKLPEKTSPGQII
                     AEEPAGYSVPNRREERVSRIIRDTAQARAIKALYDYRCQMCGTRLECPAGLYAEAAHI
                     RPLGTPHNGPDTKDNILCLCPNHHVLFDNGAVSVADDLSLIGDDSNLTVHRDHHINPH
                     YLAYHREHFLVPLEV"
     gene            2213..2821
                     /locus_tag="VHM69_09730"
     CDS             2213..2821
                     /locus_tag="VHM69_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX2740717.1"
                     /translation="MLKERHALEELARRLGGAFRAGDAAGAGVHPRVLYALRDEGFLV
                     ELSRGVFRIADAEMSPYLDLVAVSRRSPQGAICLNSALSFWDLTDEVPAEVHLAVPRG
                     AHRPRISYPPVRVHVFAAGTFELGRERVRLDSGEEIHVYSPERSVVDAVRLRGRVGTD
                     VAYEALRRYLRRPGASSGELLRLARRLRAGGPMASALEVLTG"
BASE COUNT          606 a          863 c          909 g          599 t
ORIGIN      
        1 gcaaggccgt accgctcggc tgggacgatg ttgacaccga ccagatcgtg ccttcagacg
       61 ccctcaagcg catcgagcgc acagggttcg ggcagttcct attctccgcc tggcgcgagg
      121 accccgactt catcctcaac aagcccgagc acgatggggc tgtcgtcctc atcgccggca
      181 agaacttcgg cagtggctcc agccgcgagc acgcgccgtg ggccatacag gactacgggt
      241 tcggggctgt catagccgag tccttcgcgg atatcttcaa gaacaactgc gcaaagatcg
      301 gacttctagc ggtggagttg cccgaggatg ctgtacagaa gctactcgaa gccatccgct
      361 ccgatcctga ggccggagtc tccgtcgacc tcgaagggcg cgtcgtgaag ggccctggta
      421 tcgaggagcc attcgccttc gacaacttcg cccgctacag gctcctcaac ggcctcgacg
      481 acatcggcct cacgctcacg cacgaggacg agctcgagga gttcgagaaa acgcgtctgg
      541 actatcttcc acgcgtgcag tagccccggc acccagactg tgccatattg gcgtggtgtc
      601 cttcgacgag aggtggtagc gagatggcca agaaggcgga caaggcacta aagagcctca
      661 agaaagacgt agccaagcta aagaaacaga acgacaaact cgccgagacg ctggagaagg
      721 cgcgagaaga tcaggcgtcg gcccacattg agctccgcaa gctcctcgaa gagcatctga
      781 caacgcacga cgtcggtcct gacgagtcgg tgcctgaagc tgacgagtcg acggctgtag
      841 acagctcacg cgacggcgaa ggtgaagaag agcctgaggc cactgaagaa caaccggagg
      901 ctaccgaaga agagcctgag gttaccgaag cggcgaagcg gcgggccaaa gagttcggcg
      961 tggacctctc tagcgtgaaa ggcacgggct cgggagggcg cattctcgtc aatgacgtgg
     1021 aggccgctgc ggaagcgaaa caataggtat cccggagata ctcacgtcca gagagttcac
     1081 acttcgagag gcactaggaa gtgttctcga tggtaggcga gatagtgcgg attgatgtgg
     1141 tggtctctgt gaacggtgag gttgctatca tcgccgatca aggataaatc gtctgcaacc
     1201 gaaacagcgc cgttgtcgaa gagcacatga tggttaggac acaggcagag aatgttgtct
     1261 tttgtgtccg gtccgttgtg cggagtccca agcgggcgta tgtgagcagc ttctgcgtat
     1321 agacctgcgg gacattccag acgggttccg cacatctggc acctgtagtc gtaaagtgct
     1381 ttgattgctc ttgcctgcgc cgtatctctt atgatccgcg ataccctctc ttctcggcgg
     1441 ttcggaaccg agtacccggc tggttcctca gcgattatct gaccagggct cgtcttttct
     1501 ggaagcttgg ttagacggaa acgccagatt tcgaatccag acttaccagc ctcatgccag
     1561 aagtcttcga ctgcgtacaa cccgtcgtag ctgtagccgg agggtggtga atacggggag
     1621 tcgtggttgg aacctctaat aacgcgaaca ggaaggccgt tctgcttgct aagcgcgaga
     1681 gcacggtttc cgcgggcgaa gggttgatcg tgggtcacct gtccggtact tgtgtctctc
     1741 cctccgtgcc ccgtgtagat gatcgtatca ccgtagtccg catcgtcttc gtagccgccg
     1801 gagaggacta ttgattctgc tccctctgtc tgacttccag atattcccgc ctgagttggt
     1861 acgtggaccc tgctcgcatt tagttcagcg cggctctcga agaggctccc ttcaggatac
     1921 ccaggcacgt gtccgaaaag ccgttccggc acgatgatgc ctaatagacg tgagggctcg
     1981 acaccatcac aacgtgcatt gtattcggca actctccttt cgcgcagccg acccgaaccg
     2041 ctcaaaaact ttcgcgaagt ttctcaattt ctctacgtta ccgcaacgct tcatatttta
     2101 cggtgttcct cacacatgag ggatacctca gccctacaga aggaaacctg acgaatgcgg
     2161 aatggatgaa aacctacaca cataagactt agttgtgtag acttttatcc agatgctaaa
     2221 ggagcgacat gcattagagg agcttgcccg gcgtttgggt ggagctttca gggcggggga
     2281 tgcggcggga gccggcgtcc atccgagggt tctgtatgcc ctgcgggacg agggcttcct
     2341 ggtcgagcta tcgcgtggcg tcttccgtat tgcggacgct gagatgagcc cttacctgga
     2401 cctggtggcc gtctcacggc gcagccctca gggagctatc tgtctcaact cggcgctctc
     2461 tttctgggac cttaccgacg aggtccccgc tgaagtccac ctggcggtgc ccaggggggc
     2521 gcaccggccc aggatctcct acccgcccgt ccgcgttcac gtcttcgccg ccggcacctt
     2581 cgagttgggg cgcgagcggg tccgcctcga ttcgggggag gagatccacg tctactcccc
     2641 ggagcgcagc gtggtggacg ccgtgcgcct caggggtcgc gtggggaccg acgtcgcata
     2701 cgaagccctc cgccgctact tgaggcgtcc cggggcttcc tcgggcgagt tgctacggct
     2761 tgcgaggcgg ctcagggcag ggggacctat ggccagcgcc ctggaagtac tgacgggttg
     2821 agcgagcccc agacgtcgca ggaagtctac cgagcgttgc agcgccgggc tagacacgag
     2881 ggacggggta cccaggagct cttcgagttc tacctgctcg aacgcttcct ctacaggctc
     2941 tccgtctccc ggtaccgcta ccgtttcgtg ctcaagg
//
LOCUS       DATAIR010000400         5829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_150601, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000400 DATAIR010000000
VERSION     DATAIR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150601"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..783
                     /locus_tag="VHM69_09735"
     CDS             154..783
                     /locus_tag="VHM69_09735"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX2740718.1"
                     /translation="MSLGAISDAGARGLLDPDSPVRRHYEDLANNLLRLGAGSVVVTS
                     PEPGAGRTSVCLGLGAALAGMGRRAAIVDCNLENPQLHRILGEPNFVGLTSGLEGSKP
                     LEHYGHEVIPGLLVVPTGPVLLDPAYRLEDERVGDVVRGLQKTRDLVLLDAPVAGSVL
                     RSPNLSGGFDGVLLVVHASKTPKSVARGTTDDLLEAGVNLLGVVLNGCS"
     gene            809..2431
                     /locus_tag="VHM69_09740"
     CDS             809..2431
                     /locus_tag="VHM69_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2740719.1"
                     /translation="MKEVQSSDDARGRFFGAGGEENGGKQHRPGRLLAFFKGLVLLAL
                     VGAATYGILRDGLYDDELWLPVGAAILALLFVTLFVRGFYMGLPQAGMILVALLAALV
                     AVKGISMVWTISETETIKETLRSSMYLATFLMTLAALTSGRQAGPLIDVSVVIVAAVA
                     GYGLLQKISPVEYPVQSLDGVRVDSTLAYPNTAAVILGMGAVLALARMTMMRNFLLRG
                     LYAVLALSFMITLYLTVSRGGIGSLGIGLIVLFVLGSRRLQMLANLLLLSIPGVWLWW
                     QMQSLPGLLATDATGQEKLADGTAFRNDLIVALIVAFVLQAGYAFVVNRYELLPAPRR
                     WLRIIAGVGLVLAACAGAFVVVAGYGGVGAAYEALVSNPNQTGNLGQRLVSFGIGFRE
                     DYWRVAWEAWKELPLTGTGAGTFQYTWLQDRPGVQGVKQVHNLYLEQGTETGLFAFLA
                     LMGFVAVLLGYTGKAVWRSRGERRLLLAGLVSALVVYLVSSVFEWHWYLPASTMFFFI
                     LAAVAVRFASREDWGVAEADTPPANGGPTRAD"
     gene            2465..4003
                     /locus_tag="VHM69_09745"
     CDS             2465..4003
                     /locus_tag="VHM69_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX2740720.1"
                     /translation="MNADYLRSEDGYDTQVERVARGAGVSSAGQGVGRCLGYATHVAV
                     AWMHGPAQLGFYALGITVVQVASILSQLGLDNGVVRFVAHHGAGGDTARVRGTILQSL
                     AVTLALSVALSALIFAGAGFLAEDLFGKPFLATMFRAFAAALPFLTFMSMALWVTQGF
                     GTLKYAAFVGQVARPLANLVLLVFFYLLGVQILGAVAAYVLSMAFGAALALACLPRVF
                     PGLLAGKAKYESRELSAVSAPMIVANVTQYSNLWTAVVVLGIFEPVPTVGVYSAAART
                     AALSTLVLIAFGGVFSPLAAGLYRRERLDELGRLYGDVSRWTFTGALAFFLVTALLAP
                     DIMLFFGEEFAHGWPVIVVISAAQLFNSSMGPTARLLAMTGHQKAVMVSTVGSAVAAV
                     ALNLLLVPAYGVAGAAAATASALILANGSALIFVRRRLGFWPYGRGYVKPLLAGLAAA
                     AVTLAVRHLLPALDGFPALLVFAPLALTVFFGVLVSLGLYPSDRRFLASFGSAVRDTL
                     WWER"
     gene            3991..4917
                     /locus_tag="VHM69_09750"
     CDS             3991..4917
                     /locus_tag="VHM69_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2740721.1"
                     /translation="MGAVIRTPLTRMFGGLFIDLGKGDHRNSVFLAGSGRSGTTWLSE
                     LINHRGRYRYVFEPFNPEKVSDFRHFRSKQYLRTDDRREEFLGPARRVLTGEIRDPWT
                     DRFNRTFVARRRLIKDIRANLLLGWMRANFPGMPIILLLRHPCAVVASRLALGWKDNL
                     SETMEQDDLVEDFLLPMETEIRAAGDDFERHLFLWCIDNYVPMKQFEPGKIHLAFYEN
                     LLVNPEEELRRLFDFLDEDFDGRVYARLARPSPLSRKDTPAPSVDEWRAHTSPSRLKR
                     SVEILNLFGLDRLYGEGAMPDPSGAFALMDGE"
     gene            4924..>5829
                     /locus_tag="VHM69_09755"
     CDS             4924..>5829
                     /locus_tag="VHM69_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740722.1"
                     /translation="MRKPPVVILSGVRWNFLWQRHQTLATLFARAGYPTVFVETTGLS
                     NPRLSMKTLRKLLSRVRRSGGRKSPGEKNLTIYAPLVAPPTWKAFRWSNARLFLPRVL
                     RDIGKIAGLHPVVVAYPPTRTTLDVISGLDPALVLYDCSDDYEHFPGVPEDIARTERE
                     LLLRADLVSCTSPYLLEKARHVRPDAFLSGPAVDYGRFAVLQDQRPDREVSTVCFFGD
                     VSRERIDFDALEAIVEAGFTLRIVGGVDGSLMQMPGVDYRGEVSHAELPEALAGVDAF
                     VLPYRINALTRGISPAKTYECLATVK"
BASE COUNT          913 a         1877 c         1906 g         1133 t
ORIGIN      
        1 cagtggccgg gttgctgctc gggggcaccg tggcactgct tttggaggga agggccagtg
       61 gctggcgggg tgtgcgtgac gccgaattga cgctgagggc gccggtgctt ggggccatac
      121 ctgactactc ttcgaagggg accgagggtt gacgtgagcc tcggggcgat aagcgacgcg
      181 ggcgccaggg gcttactcga ccccgactct cccgtgaggc ggcattacga ggacctggcg
      241 aacaaccttc tcaggcttgg ggctggcagc gtcgtcgtaa ccagtcccga gccgggagcc
      301 gggcgcacga gcgtctgcct cggcctcggt gccgccctgg ccggcatggg gaggcgggct
      361 gccatcgtgg actgcaacct cgagaacccg cagttgcaca ggatactagg tgagccgaac
      421 ttcgtggggc ttacaagcgg tctggagggt agcaagcccc tcgaacacta cgggcatgaa
      481 gtcatccctg ggctgctcgt cgtcccgacc ggtcccgttc tgttggaccc cgcctaccgt
      541 ctcgaggacg agagagtcgg agacgtcgtg cggggcctcc agaagacccg ggatctggta
      601 ctgctcgacg cgccggtggc ggggagcgtg ctgcgctcgc cgaacctctc gggagggttt
      661 gacggggtct tgctcgtcgt ccacgcctca aaaaccccca agagcgtcgc gcgcgggacg
      721 actgacgacc tgctggaagc gggggtcaac ctgctcgggg tggtgttgaa cggatgttcg
      781 tgaagcccgc tcgtcgtggg gtagaggggt gaaagaggtc caatcctcag acgacgccag
      841 aggacgcttc ttcggcgccg gcggcgagga gaacggagga aagcagcacc gtcctggccg
      901 tctccttgcc ttcttcaagg gcctcgtact gcttgctctg gtgggagcgg caacttacgg
      961 gatactccga gacggcctct acgacgacga gctctggcta ccggtcggcg cggcgattct
     1021 tgcgctgctt ttcgtgacgc tgttcgtgcg cggtttctac atgggcctgc cgcaggcagg
     1081 gatgattttg gtcgcgctcc ttgcggcgct cgtagccgtg aagggaatct cgatggtctg
     1141 gaccataagc gagacggaga ccatcaaaga gacccttcgc tcctcgatgt acctggcgac
     1201 cttcctcatg acgctggcgg cgctcacctc gggacgccag gctggtccgc tcattgacgt
     1261 ctcggtcgtc atagtcgctg ccgtagccgg ttacgggctc ctgcagaaga tcagccccgt
     1321 cgagtatccc gtccaatccc tcgacggggt gagggtggac tcgaccctgg cgtacccgaa
     1381 tacggcggct gtgatcctcg ggatgggggc cgtgctggcg ctggcgagaa tgaccatgat
     1441 gcgcaacttc ctcctcaggg ggctctacgc ggtcctggcg ctctccttca tgatcacgct
     1501 ctacctgacc gtctcgcgcg gcggcatcgg gtcgttgggg ataggtctga tcgtcctctt
     1561 cgtcctcgga agccgccgtc tgcagatgct cgcgaatctg ctccttttgt ccatcccagg
     1621 cgtctggctg tggtggcaga tgcagagcct tcccgggcta cttgcgacag acgccaccgg
     1681 tcaggagaag ctcgccgacg ggacggcgtt caggaacgac ctgatcgtgg ccctcatcgt
     1741 ggcgttcgtt ctgcaggccg gttacgcctt cgtcgtcaac cgctacgagc tgttgcccgc
     1801 gccgcggcgg tggctcagga taatcgccgg tgttgggttg gtgctggcgg cctgcgcggg
     1861 cgcgttcgtg gtcgtcgctg gatacggtgg ggtcggggcc gcctacgagg cgctcgtcag
     1921 caacccgaac cagacgggaa acctcggcca gaggctcgtc tccttcggga taggattcag
     1981 ggaggactac tggagggttg cgtgggaggc gtggaaagaa cttcccctga cggggacggg
     2041 agcaggcacc ttccagtaca cgtggctgca agacaggcca ggggttcagg gcgtcaaaca
     2101 ggtccacaac ctgtacctgg agcaggggac cgagacagga ctgttcgcct tcctggccct
     2161 gatgggcttc gtggctgtcc tccttggcta tacgggtaaa gccgtctggc ggtcgcgggg
     2221 agagcggagg ctgctcctcg ccgggctcgt ctccgccctc gtcgtctacc tggtctcctc
     2281 cgtattcgag tggcactggt accttcctgc ctcgacgatg ttcttcttta tcctcgccgc
     2341 cgtcgccgta aggttcgcct caagggagga ctggggcgtc gccgaggctg acacgcctcc
     2401 cgcgaacggt gggcccacgc gggctgattg aagccgcggg acagagaaaa tatcatcggg
     2461 ttcgatgaac gctgattatc tccggtcgga ggacggctac gatacccagg tcgagcgcgt
     2521 ggccaggggg gctggcgtca gctccgcggg gcagggcgtt ggccgctgcc tcggctacgc
     2581 gacgcacgtc gctgtggcgt ggatgcacgg ccctgcccag ctcggcttct atgcactggg
     2641 gatcacggtc gtgcaggtgg cgagtatcct ctcgcagctc gggctggaca acggcgtggt
     2701 gcgcttcgtc gcccaccacg gggccggagg cgacacggcg agggttcgcg gcaccatact
     2761 gcagtccctc gccgtaaccc tcgcgctcag cgtggcgctc tcggccctga tcttcgcagg
     2821 ggcgggattt ctggcggagg atttgttcgg gaagcccttc ctggcgacga tgttccgggc
     2881 gttcgccgcc gcccttccct tcctcacgtt catgagtatg gcgctctggg tgacccaggg
     2941 cttcggtacc ctcaagtacg cggcgttcgt ggggcaggtg gcaagacctc tggcgaacct
     3001 ggtgctcctc gtcttcttct acctgctcgg cgtccagata ctcggggccg tcgcggctta
     3061 cgtgctctcg atggccttcg gcgcggccct cgcgctggcc tgcctgccgc gcgtgttccc
     3121 gggtttgctc gcaggcaagg caaaatacga gagcagggag ctctccgccg tctctgcgcc
     3181 catgatcgtc gccaacgtga cccagtactc gaacctgtgg accgccgtcg tcgtgctcgg
     3241 tatcttcgag cccgtgccga ccgtcggcgt ctacagcgcg gcggcccgca cggcggcgct
     3301 ctctaccctc gtcttgatcg ccttcggcgg agtcttctcg cctctcgcag ctggcctcta
     3361 ccgtagagaa cgcctcgacg agcttggccg cctctacggg gacgtctcgc gctggacctt
     3421 caccggcgcc ctcgccttct tcctcgtcac cgcgctcctc gcgccggaca taatgctctt
     3481 cttcggcgag gagttcgccc acggctggcc tgtgatcgtg gtgatctccg ccgcccagct
     3541 cttcaactcc tcgatgggcc cgacggcccg cctgctcgcg atgacgggcc accagaaagc
     3601 cgttatggtc tcgacggtag gctccgcggt cgcggccgtg gcgctgaacc tcctgctcgt
     3661 gccagcatac ggggtcgcag gcgcggcggc ggccacggcc tcagccctca tcctggcgaa
     3721 cggttcggcc ctgatcttcg tccgccgcag gctcggcttc tggccctacg gccgcgggta
     3781 cgtgaagccg ctacttgcag gtctcgcggc ggccgcggtg accctcgcag tcaggcattt
     3841 gctccccgct ctcgatggat tccctgcgct gctcgtgttc gcgcccctcg ccctcaccgt
     3901 cttcttcggt gtgctcgttt ccctgggtct ctatccgagt gatcgccggt tcctcgcttc
     3961 cttcggtagc gcggtaaggg ataccctctg gtgggagcgg tgatccgcac cccgctcacc
     4021 aggatgtttg ggggcctatt catcgatctc ggtaaaggag accataggaa ctccgtcttt
     4081 ctggccggga gcggcagaag cgggaccacg tggctctccg agctaatcaa ccaccgtgga
     4141 aggtatcgct acgtcttcga gccattcaat cccgaaaagg taagcgactt caggcacttc
     4201 aggagcaagc agtacctgag gacagacgat aggcgggagg agttcctggg accggctcga
     4261 cgtgtgctaa ccggtgagat cagggacccc tggacggaca ggttcaacag gacgttcgtc
     4321 gccaggcggc gtctgatcaa ggacataagg gccaacctgc tcctcggctg gatgcgcgcg
     4381 aattttccag gtatgccgat catccttctt ctcaggcatc cctgcgccgt cgtcgcctcc
     4441 aggctggccc tggggtggaa ggacaacctc tcggagacga tggagcagga tgacctggta
     4501 gaggactttc tcctccccat ggagacggag atacgggccg ccggcgacga cttcgagcgc
     4561 cacctcttcc tgtggtgcat agacaactac gtccccatga agcagttcga gccgggcaag
     4621 atccacctcg ccttctacga aaacctgctg gtgaaccccg aggaagaact acgtcgcctt
     4681 ttcgactttc tggacgagga tttcgacggt cgcgtctacg ccagactggc acgaccttct
     4741 cccctgagcc ggaaggacac acccgctcct tccgtggacg aatggcgtgc ccatacgagc
     4801 cccagcaggc tgaagaggag cgtcgagata ctgaacctct tcggcctcga ccggctctac
     4861 ggcgagggag ccatgcccga cccctccggg gcctttgccc tgatggacgg ggagtaggca
     4921 ggcgtgcgca agccgcccgt ggtgatcctc tccggcgtca ggtggaactt cctctggcag
     4981 cgacaccaga cgctcgccac cctctttgcc agggccggct accctaccgt cttcgtagag
     5041 accacggggc tctccaatcc tcgcttgagc atgaagacgc tacgaaagct gctctcccgg
     5101 gtccgccgtt ccggaggacg gaagtcgccg ggagaaaaga acctcaccat ttacgcgccg
     5161 ctcgtagccc cgccgacatg gaaggcattc cggtggtcga acgcgaggct tttcctcccg
     5221 cgggtgctcc gcgacatcgg gaagatagcc ggtctccatc ctgtcgtcgt ggcctacccg
     5281 cctacccgta cgacgctaga tgtgatctcc ggcctcgacc ccgccctggt cctctacgac
     5341 tgctcggacg actacgagca cttccccggc gtgccagagg acatcgcaag gacggagcgc
     5401 gagctcctgc tgcgcgccga cctcgtctca tgcacctcac cctacctgct tgagaaggcg
     5461 aggcacgtca ggcccgatgc cttcctgagc ggcccagccg tggactacgg gcggtttgcg
     5521 gtcctgcagg accagcgtcc cgacagagag gtcagcaccg tgtgcttctt cggtgatgtg
     5581 agcagggagc gcatagactt cgacgccctg gaggccatcg tcgaggcggg tttcacgttg
     5641 cgaatcgtgg gcggcgtgga cgggagcctc atgcagatgc caggagtaga ttacagggga
     5701 gaggtctccc acgcggagct tccggaggcg ctcgctggcg tcgacgcttt cgtattgccg
     5761 tacaggatca acgctctcac gaggggcatc tcgccagcca agacctacga gtgcctggcc
     5821 actgtcaag
//
LOCUS       DATAIR010000401         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_118641, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000401 DATAIR010000000
VERSION     DATAIR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118641"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..875)
                     /locus_tag="VHM69_09760"
     CDS             complement(195..875)
                     /locus_tag="VHM69_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HEX2740723.1"
                     /translation="MSESAEQDFLLALMVRTPDEPGALHALTRVLLDHKANITYVDIA
                     ERREGLSTVYFELEDVASEGADVLIGDLESLSIVRAVERAPSFQKVYGKRIIVVGGGA
                     QVGQVVVGAVAEADRHNIRGEKISVDTIPLVGEEELAAATRAVARLPRAVALVLAGAL
                     MGGEVARAVREIREKGIIVLSLNMPGSVPDAADLVVSDPVQCGVMAVMAVSDSALFDI
                     ARQRGRRY"
     gene            complement(921..1403)
                     /locus_tag="VHM69_09765"
     CDS             complement(921..1403)
                     /locus_tag="VHM69_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740724.1"
                     /translation="MIWFAYFMLALESLVIAITLWMMWAMISEASRAPAEPFPPAEPP
                     PEAGDDNSLFTLEEVDERGYLSSRKLHMGCEAAVCRTPSGARIVAGRRSLKEPQYAWD
                     YASVELAQKALVYAEFNTHLEDGIIGVEDLGRGSYPAAEPKEYLRRWRYAAGGPMELF
                     "
     gene            complement(1580..1843)
                     /locus_tag="VHM69_09770"
     CDS             complement(1580..1843)
                     /locus_tag="VHM69_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX2740725.1"
                     /translation="MADVHVYVSRPAGKEGTGLEDLEAVLKKLEHVSAVEVNPSGNVV
                     AVSFEGGKAEQEEIKHAIEEAGYEISRLSVRSDFSEDRNLWDI"
     gene            complement(2090..2842)
                     /locus_tag="VHM69_09775"
     CDS             complement(2090..2842)
                     /locus_tag="VHM69_09775"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740726.1"
                     /translation="MFLELPERVAIVTGAARGIGFAIAERLSKAGARVVVADVDREGA
                     VEAVNRLREGGAQAVEAIADITDPEEVDAMVERTIDSFGRLDVLVNNAGITGRDAPLS
                     GITDEDWERVLRLNLTATFYCCRAAIPHMRERRSGAVVNVASISGKEGNPNMIPYSVS
                     KAGVICLTKALAREVIDEGVRVNCVAPAVIETPLLDQLQSEAIDYMTSKIPMGRMGRP
                     DEVAAVVHFLASDDASFVTGQCYDVSGGRATY"
     gene            complement(2924..3868)
                     /locus_tag="VHM69_09780"
     CDS             complement(2924..3868)
                     /locus_tag="VHM69_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX2740727.1"
                     /translation="MSRHILIDGGQSGCRIVYVANGERIMVGAGAGLGRQDPDRTEGL
                     LRVLESTFEDMAPRIPDTVDVVAAGLTGFDGSSEAARAIADGVRKRVRTARVVVTDDA
                     VTSYLGAIGFEPGVVVAAGTGVIALAGDREGNVARSDGWGYILGDDGSGYYIGRRGLA
                     SALRSHDGRGGSEELLRRAKEVFGAPGSLKDQVYTSSNPAGEVAKFAREVAEAARGGD
                     VAASEIWAIAAREVAQTATAALDQVFTPGTSVTVSWTGNLFEAGDLMLQPFQRQVAVM
                     WPAARLLTPKGAALDGAELLARSHAPHMFEDLLHVFES"
     gene            complement(3875..>4834)
                     /gene="nagA"
                     /locus_tag="VHM69_09785"
     CDS             complement(3875..>4834)
                     /gene="nagA"
                     /locus_tag="VHM69_09785"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX2740728.1"
                     /translation="QINGAFGIDVAPDPEALKALARKLPRTGTTSFLPTLISSPLEVY
                     ADFLDALEDSMRPSGSRILGAHLEGPFLSPARKGAHDSANLRPVDLGLAKEFIRSGVV
                     RMVTLAPELSDAREAVRLFLEGGVVPSTGHTDATYEEILRSMDSGLRMGTHLYNAMSP
                     FAHRAPGTVGAMLADDRARAGIIVDGVHAHEGALRVAYRQKGPEGLALVTDAMEAAGM
                     PSGEYELSGRKVRLDGGAVRLPDGTLAGSALTMDVAVHNAARMLHIPLHDAVRMATQT
                     PAEILGMPAKGRIIPGADADLVVLGQEGRVEETIVGGESAYAR"
BASE COUNT          862 a         1620 c         1421 g          931 t
ORIGIN      
        1 tacgttctcc cccaccggag agaccaatcg tctgttggcg aacgaagggt gctccggcta
       61 tcgaatctat cctctgcgta taacacaaca cgccgcggga cgcgagtatt ggcgcttcgt
      121 gcagctacga cagcgcataa aggagataga ccttacagga tgtcttacca ggatcagcca
      181 tggtatctcc cgacctaata gcgccggccc cgttggcgag cgatgtcgaa gagagcggaa
      241 tccgagaccg ccatcacggc catcacgccg cactggaccg ggtcgctcac cacgagatcg
      301 gcggcatcag gtacgctgcc tggcatgttc aacgagagca cgatgatccc cttctcccgg
      361 atctcgcgta cggcacgggc gacctcaccg cccatcagcg cgccggccag aaccagcgcc
      421 accgcccgcg ggagacgggc caccgcgcgc gtcgcggcgg cgagctcttc ctctccgacc
      481 agggggatgg tgtccacgga gatcttctct ccgcggatgt tgtgcctatc ggcctccgcg
      541 accgcgccaa ccaccacctg acccacctgc gccccgccgc cgacgacgat gatccgcttg
      601 ccgtacacct tctggaagga gggcgcccgt tcgaccgcac gcacgatgga cagcgactcg
      661 aggtcgccaa tgagcacgtc ggcaccttcg ctggccacgt cctcgagttc gaagtagacc
      721 gtcgagagtc cctcgcgtcg ctcggcgata tcgacgtagg tgatgttcgc cttgtggtcg
      781 aggagtaccc tggtaagcgc gtgcagcgcg ccgggctcgt cgggcgtgcg aaccatcagg
      841 gccaggagga aatcttgctc ggcgctctcg ctcatggaca gcagtttata gcctcggtgg
      901 ctacccggcc cgatcacact tcaaaaaagt tccattggtc cgccggctgc gtatctccac
      961 cgacgcaggt actccttggg ttctgctgcc ggataggagc cacgccccaa atcttcgacg
     1021 ccgatgatgc cgtcttccag gtgagtgttg aactcggcat agacgagagc cttctgtgcc
     1081 agctcaacgc tggcatagtc ccaggcgtac tgcggttctt tcaaagagcg tctgccagcg
     1141 acgatccttg caccggaagg agtacggcag actgccgcct cgcaacccat gtggagcttc
     1201 ctgctgctca agtagccgcg ctcgtcaacc tcctccaggg tgaacagaga gttgtcgtct
     1261 ccggcttcag gcggaggctc cgccggaggg aatggttcgg cgggcgcccg ggacgcttcc
     1321 gatatcatcg cccacatcat ccacagggtg atggctatca ccaggctctc cagcgcgagc
     1381 atgaagtaag cgaaccagat catctcgcac cctcgaggtc gcccacagtg cccaccaccg
     1441 ccataatagt ccgctctccg ctagtatatt ctacgccctc cgggacaaaa cgcggcgcgg
     1501 caacgccggg gaagaaggtc tctctctgga aagttcctca gcgcgcgacc tcggctccca
     1561 cattctcatg ggagggactt cagatatccc agagatttcg atcttctgag aagtccgacc
     1621 tgacagagag cctggatatc tcgtagccgg cctcttcgat ggcgtgcttg atctcctcct
     1681 gctcggcctt ccctccctcg aatgagacgg ccaccacgtt accggacggg ttgacctcaa
     1741 cggcggagac gtgctccagc ttcttcaaga ccgcttcgag atcttccagg cccgtaccct
     1801 cttttcccgc cggtctactg acgtagacgt gtacatctgc cacagttcgt tcctttctct
     1861 caatcggttc gggcgaagaa tacaggattc aggtttcagg cttcaggttt caggtatttg
     1921 gtttggttgc ggcgtgggat ctcgttcgcc gatgagggtg ggcgatggat cgcctttcat
     1981 cattcccagc agaggaggcc gccaagacaa aggctcaaac actgaaagcg aagggcgtag
     2041 cgtgctgaca tgccgcgaat agcagaagtc atgacttctg ctattcgctt tagtacgttg
     2101 cccttccgcc gctgacgtcg tagcactggc ccgtgacgaa gctcgcatcg tctgaggcaa
     2161 gaaagtgcac taccgccgcc acctcatccg gtcttcccat ccgccccatc gggatcttgc
     2221 tcgtcatgta gtcgatggcc tcggattgga gttgatcgag cagtggcgtc tcgatgacgg
     2281 cgggagcaac gcagttcacc ctcacgcctt cgtctatcac ctccctggcc agcgccttgg
     2341 tcaggcagat caccccggcc ttggagaccg agtaggggat catgttcgga ttgccttcct
     2401 tgccagagat ggacgccacg ttgaccacgg caccgctccg acgctcgcgc atgtgaggga
     2461 tcgcagcccg acaacagtag aaggtggctg tcaggttgag gcgcagcacc ctctcccaat
     2521 cctcgtctgt gatcccggag agcggcgcat cgcggcccgt tattccggcg ttgttcacca
     2581 gcacgtcgag cctgccgaaa gagtcgatgg tacgctcgac catcgcgtcg acttcctcgg
     2641 gatctgtgat atccgcgatc gcctcaacgg cctgtgctcc cccttcgcgc agcctattta
     2701 cggcctcgac ggcgccttct ctatcgacgt ccgcgaccac aacgcgcgcg ccggccttcg
     2761 agaggcgctc cgcgatggcg aatcctatgc cccgcgcagc cccggtgacg atcgcgaccc
     2821 tttccggcag ttccaggaac acggtccggc tcctctctct agctctcatc gcccggttac
     2881 gatcttacat tctgcgctga gataacccgc atcgctagcg aagctaactc tcgaacacgt
     2941 gcaacaggtc ttcgaacatg tgaggcgcgt gcgaacgggc gagcagttcc gccccgtcaa
     3001 gggccgcgcc cttcggggtg aggaggcggg ccgccggcca cattaccgct acctggcgct
     3061 ggaaaggctg gagcatgaga tccccggctt cgaacaggtt tcccgtccac gaaacggtca
     3121 cggaggtgcc gggagtgaag acctggtcta atgcggctgt cgccgtttgc gccacctcac
     3181 gcgcggcgat ggcccatatc tccgaggctg ctacatcgcc tccacgcgcg gcctctgcga
     3241 cctccctggc gaacttcgcc acctcgccgg cgggattgga agaagtgtag acttgatctt
     3301 ttaacgatcc gggcgcgccg aacacttcct tcgcccttcg cagtagctct tcagagcctc
     3361 ccctgccgtc gtgcgagcgc aacgcgctgg cgagcccgcg cctccctatg tagtagccac
     3421 taccgtcgtc gcccaggatg tacccccagc cgtcgctacg ggcgacgttc ccttctctat
     3481 cccctgcgag ggctatgacc ccggtaccgg ccgcgaccac cacccccggc tcaaagccta
     3541 tggcgcccag gtaggaggtc acggcgtcgt ccgtcacgac cacgcgcgcg gtccgtaccc
     3601 gctttcgtac gccgtccgct atcgcgcgag ccgcctcaga ggagccgtcg aatccggtaa
     3661 gcccggctgc aactacgtcg acggtgtctg gaatacgcgg cgccatgtcc tcgaaggtac
     3721 tttcgagcac acgcaacagg ccctcggtac ggtccggatc ctggcgacca agcccggctc
     3781 cggctcccac catgatccgc tctccatttg ccacgtagac tatgcggcag ccgctctgtc
     3841 ctccgtcgat aaggatgtgc ctgctcatct tcggtcaccg tgcataggcg gactcaccgc
     3901 caacgatggt ctcttctacg cgtccctcct gcccgagcac gacgaggtcg gcgtcggccc
     3961 cggggatgat cctgcccttc gcaggcatcc cgagtatctc agcaggggtc tgcgtcgcca
     4021 ttcggacagc gtcgtggagc gggatgtgaa gcatccgcgc ggcgttgtgt acggctacgt
     4081 ccatggtgag ggcgctgccc gccaacgtgc catcaggcag gcggacagct ccaccatcga
     4141 gacggacctt tctgccgctc agctcgtact ctccggacgg catgcccgca gcttccatcg
     4201 cgtcggttac gagggcgaga ccttccgggc ctttctgccg gtaagccact ctcaatgcgc
     4261 cttcgtgcgc gtgcaccccg tcgacgatga tgccggctcg cgcgcgatcg tcggcgagca
     4321 tggctccgac ggtgccggga gcgcggtggg cgaaggggct catggcgttg tacagatgcg
     4381 tacccatccg caagccggag tccatcgaac gcaggatctc ctcgtaggtt gcatcggtgt
     4441 ggccggtgct cgggactacc cctccttcta ggaacagtcg taccgcctcc cttgcgtccg
     4501 aaagttccgg cgcgagtgtc accatacgta ccacgccgga acggatgaac tccttcgcga
     4561 gtccaagatc cacgggccgc aggttggcag agtcgtgggc acccttgcgc gcgggcgaga
     4621 ggaacggacc ctccaggtgg gccccgagaa tcctcgaacc ggatggtcgc atactgtctt
     4681 cgagggcgtc cagaaagtcg gcgtaaacct ccagagggga ggagatcagg gtgggcagga
     4741 acgaggtggt gcccgtccgc ggcagcttgc gggccagtgc tttcagggct tccgggtccg
     4801 gagctacgtc tattccgaag gcgccgttga tctg
//
LOCUS       DATAIR010000402         6165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_440988, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000402 DATAIR010000000
VERSION     DATAIR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440988"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1369
                     /locus_tag="VHM69_09790"
     CDS             62..1369
                     /locus_tag="VHM69_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX2740729.1"
                     /translation="MPEVIMPKMGDAMEEGTLLKWLKSEGEEVSEGDPIAEIETDKVT
                     MELEAEDAGTLAQLIASEGQDVPVGEAIAFIQGEGEEVPERDGGASAEAEQEEGGDEG
                     GGEAQTATEAPEEEEPSGDGARATSDGHFRASPIVRRLADENDLDLSKIDGSGPAGRI
                     VERDVRAAMESGTAQRTEEEQAEAQPQQAAPQGFQPAHVPEPTEAPGTQLIEPTRMQR
                     VIGERMTEAKQNIPHFYASVEVRMDAAMALRKQLNEQLEPEGIKLSVNDFVMKAAAVA
                     LRNYPNLNALYTSKGIELHEKVDMAMAVALDAGLITPVIRDIGSKGLATISRESKDLA
                     KRAREGKLQPDEYQGGTITVSNMGMFGVESFTAIINPPQAAIIAVSSIDRRPSYDENG
                     EVVPASMMKLTLSADHRIANGRDGALYMSEVKKTLENPVLLMV"
     gene            complement(1468..1830)
                     /locus_tag="VHM69_09795"
     CDS             complement(1468..1830)
                     /locus_tag="VHM69_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740730.1"
                     /translation="MDETGGLQNNVLNGGTGTDTASYASSATAVSASLATDFAMGESS
                     DLLSFIERLTGSTKADNLTVADTSTTPDFNAANVLKGLGGADTLDAAEGTGNDRVDGG
                     TGNDTCQTDMGDTKLNCP"
     gene            complement(2010..2813)
                     /locus_tag="VHM69_09800"
     CDS             complement(2010..2813)
                     /locus_tag="VHM69_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX2740731.1"
                     /translation="MLEFLRNLLDPLFSIMGATLSTFHGWGAPWWLAIVMLTIVVRTL
                     LFPLTYRQVRSMRRMQDLKPDMDRIRADYKDDVPKQREQMAKLYQERNVNPLGGCLPI
                     VIQLPIFLVLYYTIRHFDKLESFRTGGLMWFTDLTQPDHLFILPVLYILTMMASQELT
                     IRNTAPQQKQLMRFLPIVFGIFLARFPAGLFVYWVTSNLITFCQNFVIYNVLPHKTAY
                     TEVMKLSAEAGEKPTPDANPEQEKKEPPQHQKRSGRARSRKKKKVGKRK"
     gene            complement(2881..3273)
                     /locus_tag="VHM69_09805"
     CDS             complement(2881..3273)
                     /locus_tag="VHM69_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740732.1"
                     /translation="MLVWRAGLSSTFEKNLTVERALRGISFPAFGLVLFGLLVNLFSR
                     EEVYASIEYGFPMLALFGAAGCIGQLTLSISRRSSSLAFACCIPMGVPLATVLPGGVR
                     GETSDLPLLIALLFGPIVVLLAGLGGWV"
     gene            3443..5485
                     /gene="fusA"
                     /locus_tag="VHM69_09810"
     CDS             3443..5485
                     /gene="fusA"
                     /locus_tag="VHM69_09810"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX2740733.1"
                     /translation="MAVETETIRNVCLIGHRGSGKTSIAEGMIGLASGRSGPTSGVLD
                     YTEEESERGMTLGMSVANLGWKGRQVNVLDTPGDGGFVGDAFIAQKVADCAILVVHAQ
                     DPIQVVTERVWRRGEKEGIPHVIVVNHLDRERTDFGVVIEQLRERFGQAVVPLNLPIG
                     HENDLKGIYGLLSGTSFIDGEQKAEIPSGMEDEVDAAKTQLFEAIAESDDTLLEKYLE
                     GEEITTEEAFDGIRKGIADGLIIPVLATSAERMIGIDRLLDVIAGSAPSPSDRSRWIT
                     EEGEEVPCEDDGPFSAYAFKTYVDPYAGRLSVLRIVSGRCRSDEALTNPRTGSAERLG
                     GISHLVGKERNSVDEAVAGDIIAVAKLRDTATFDTLCRPEKPMSFEPVELPEPTSAFA
                     VGAKARGEEEKVFDAIRRIVDEDPSLKLERSEATGEDILAGLAQMHVELALERIEHRY
                     GVEVEARPPKVPFMETITGSAQAQGRYKKQTGGRGQFGDARIELSPLPRGSGFEFENA
                     IVGGAIPRQFIPAVEKGIIEAMAGGTIAGYPVVDVKVRLHDGAFHTVDSSEMAFKVAG
                     SMAFKNAIEKAHPVLLEPYVKVEVLAPSEMVGDIMGDLSGRRGRPMGIEQRGERQVIQ
                     AEVPQIEMLTYARDLRSITGGRANFHVESGHYEEVPPNLVDKVLAANEREEEKAVN"
     gene            complement(5544..>6165)
                     /gene="nthA"
                     /locus_tag="VHM69_09815"
     CDS             complement(5544..>6165)
                     /gene="nthA"
                     /locus_tag="VHM69_09815"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX2740734.1"
                     /translation="YHAHEEAGSVRTNSPYAARIRAIEELLIEKGILTEPEVQDRISY
                     MEARSPANGAKLVARAWVDEDFKELLLSDGKSAASELGIDPEHPAEFVVVENTPHVHN
                     LIVCTLCSCYPRAILGRPPDWYKSFDYRSRAVREPRAVMREFGFEPPEGVEVLVHDST
                     ADVRYMVLPMRPPDTENLNEEELAELVTRDSLVGVSIPRYAVSEAL"
BASE COUNT         1196 a         1809 c         2052 g         1108 t
ORIGIN      
        1 ccgagcgaga agcaggtaac gaacgcggcc cgccgggttc tgtacgagga ggagaagtaa
       61 ggtgcctgaa gtcattatgc ccaagatggg cgacgcaatg gaggagggca ccctcctcaa
      121 gtggctgaag agcgaaggcg aggaggtctc cgagggggac ccgatagccg agatagagac
      181 cgacaaggtc actatggagc tggaggccga agacgctggg acgctcgccc agctcatcgc
      241 gagcgagggc caggatgttc ccgtcggcga ggcgatagcc tttatccagg gcgagggcga
      301 ggaagtcccc gagcgcgacg gcggcgcgtc cgctgaggcc gagcaggaag agggcggcga
      361 tgagggcggc ggcgaagccc agactgcaac cgaggccccg gaggaagagg agccctcagg
      421 cgacggagcg cgggctacca gcgacgggca tttccgggct tctcccatag tacggaggct
      481 ggccgatgag aacgacctcg acctctcgaa gatagacggc tccggcccgg ctggccgcat
      541 cgtggagcgc gacgtgcggg cagcgatgga gagcgggaca gcccagagga ccgaggaaga
      601 gcaggccgaa gcccagccgc agcaggccgc cccgcagggc ttccagccgg cgcacgttcc
      661 cgagccgacc gaggcgcccg ggacccagct catagagcct acccgcatgc agcgcgtcat
      721 cggcgagcgg atgaccgagg ccaaacagaa catcccgcac ttctacgcca gtgtcgaggt
      781 caggatggac gccgcgatgg cgctgcgcaa gcagctaaac gagcaactcg aacccgaggg
      841 catcaagctc tccgtcaacg acttcgttat gaaggccgca gccgtcgcct tgaggaacta
      901 ccccaacctc aacgccctgt atacgagcaa aggcatcgag ttgcacgaga aggtcgacat
      961 ggcgatggcc gtcgccctcg acgctggcct cataaccccc gtcatcaggg acatcggcag
     1021 caagggcctc gcgaccatct cacgcgagtc caaggacctc gccaagaggg cgcgcgaggg
     1081 caagctccag ccggatgagt accagggagg cacgatcacg gtctccaata tgggcatgtt
     1141 cggcgtcgag agcttcacgg ccatcatcaa cccgccgcag gccgcgatca tcgcggtatc
     1201 cagcatcgac aggcgcccga gctacgacga gaacggcgag gtcgtccctg cgagcatgat
     1261 gaagctgacc ctctcggccg accaccgtat cgccaacggc cgcgacggcg ccctctatat
     1321 gtcagaggtc aagaagacgc tggagaaccc ggtgttgttg atggtttaga ggtttcaggc
     1381 ttcaggcttc agcaattagc tcccggtcct cgccgggggc ttcttgtttt cgtgcgggtg
     1441 tgagggcctg ggctccggcc gccgtcctta cgggcagtta agtttcgtgt ctcccatgtc
     1501 tgtctggcac gtatcgttgc cggtcccgcc gtccaccctg tcgttgcccg tgccctccgc
     1561 ggcgtccagt gtgtccgctc ctcccagtcc cttgagcacg ttggccgcgt tgaagtccgg
     1621 cgtcgtgctg gtgtccgcaa ccgtcaggtt gtcggcttta gtggagccgg tgagcctctc
     1681 tatgaaggag agcaggtcgg agctctcgcc catcgcgaag tccgtggcga ggctggcgct
     1741 cacggctgtc gcgctgctcg cgtaggaggc ggtgtccgtt cccgtcccgc cgttaaggac
     1801 gttgttctgc agcccgcccg tctcgtccac cactgggtct tccccttcat gcctcagcgc
     1861 agtataggct gcaaaaggat caagcgcgta ccacaaacga gtgattttta tcgttacgaa
     1921 tgtgttgcta tttctttaca agttggtccc ttggcggctt ctctggttag tatcgagggc
     1981 gcctgcatgt gtggtggacg ctccgatctc tatttccttt ttccgacctt ctttttcttt
     2041 ctgctccggg cacgtccgct ccttttctgg tgctggggag gttctttttt ctcctgttct
     2101 gggttcgcgt ccggagtcgg cttctctccg gcttctgcgg agagcttcat tacttctgtg
     2161 tacgcggtct tatgtgggag gacgttgtag atcacgaagt tctggcagaa ggtaatgagg
     2221 tttgacgtca cccagtagac gaagagcccc gcggggaacc tggcgaggaa gataccgaag
     2281 actatgggca gaaagcgcat gagttgtttc tgctgcgggg cggtgttcct gatggtcagt
     2341 tcctgggagg ccatcatggt caggatgtag aggacgggga ggatgaacag gtggtcgggc
     2401 tgggtcaggt ctgtaaacca catgaggcca ccggtcctga agctttcgag cttgtcgaag
     2461 tgcctgatcg tgtagtagag gacgaggaag atcgggagct ggataacgat gggcaggcat
     2521 ccgccgagcg ggttgacgtt tcgctcctgg tagagcttcg ccatctgctc gcgttgcttc
     2581 gggacgtcgt ctttgtagtc ggccctgatc ctgtccatat ccggtttgag gtcctgcatc
     2641 ctgcgcatgc tcctgacctg acggtaggtg agcgggaaaa gcaaagtacg cacgacgatg
     2701 gtgagcatga cgatggcgag ccaccatggc gcaccccacc cgtggaaggt cgagagcgtg
     2761 gcccccatga tgctgaaaag cgggtctagt aaattcctca agaattcgag catgtatcgt
     2821 cgtttccttt tgtggcttgg gtgtatataa cgcggtcccg cgagagcggg gacgcgactc
     2881 ttacacccag ccgccgaggc cggcgaggag aacgacgatg gggccgaaaa ggagcgctat
     2941 caggaggggc aggtcgcttg tctcgccacg aacgccgcct ggcaagacgg tggccagcgg
     3001 tactcccata gggatgcagc aggcgaaggc cagagagctg gagcggcgtg agatgctgag
     3061 cgttagctgg ccgatgcagc cggccgcgcc gaagagcgcg agcatcggaa aaccgtactc
     3121 gatgctggcg tagacctctt ccctggagaa caggtttacg agcaggccaa acagcacgag
     3181 cccgaaggcg gggaaggaga tcccccgcag cgctctctct accgttaggt ttttctcgaa
     3241 cgtactactc aggccggctc tccagactaa caaaacctgg cgatggttac ttgttgctcg
     3301 tggttgagtc tagcaccttt caggcaaccg gcggccggcg ttggtgtgtc tctcctgagc
     3361 ctgcgcgaag ggccttcctg cgataagcta catgccccgc ccgcggggat gataggggtt
     3421 gagcgcagga aaggaggcat ccgtggctgt agagacggag accataagga acgtgtgcct
     3481 gatcgggcac aggggcagcg gcaagacgtc gatagcggag ggtatgatcg ggcttgcatc
     3541 cggtcggagc ggtccgacga gcggcgtact cgactatacg gaagaggagt ccgagcgggg
     3601 aatgacgctg ggcatgagcg tcgccaacct cgggtggaag gggagacagg tcaatgtcct
     3661 cgataccccc ggagacgggg gcttcgtcgg ggacgccttc atagcccaga aggtcgcgga
     3721 ctgcgcgatc ctcgtcgtcc acgcccagga cccgatccag gtcgttactg agcgcgtgtg
     3781 gcgcaggggg gagaaggaag gcatccccca cgtcatcgtc gtcaaccacc tcgatagaga
     3841 acgcacggac ttcggggtcg tgatcgagca actgagggag cgcttcgggc aagcagtcgt
     3901 cccgctcaat ttgcccatcg gacacgagaa tgacctcaag ggaatctacg ggctcctgag
     3961 tggcacatcc ttcatcgacg gtgagcagaa ggcagagata ccctcaggca tggaagacga
     4021 agtggacgcc gccaagacgc agctcttcga ggccattgcg gagagcgacg atacgctcct
     4081 tgaaaagtat ctcgaaggag aggagatcac gaccgaggag gctttcgacg gcatccgcaa
     4141 gggcatcgct gacgggctca tcatcccggt cctggcgacg agcgcggagc gcatgatcgg
     4201 catagaccga ctgctggacg tgatcgcagg tagcgccccg agcccctcgg acaggtcgcg
     4261 ttggatcacg gaggaaggcg aagaggtccc gtgcgaggac gacggaccct tctccgccta
     4321 cgccttcaag acctacgtcg acccttacgc aggacgcctg agcgtcttga ggatcgtgag
     4381 cggccgctgc cgctcggacg aggcgctcac caacccgcgc accggctcag cagagaggct
     4441 cggggggatc tcgcacctcg tcggcaagga gcgcaactcc gtggatgagg ccgtcgccgg
     4501 cgacatcatc gccgtcgcca agctcaggga tacggcgacg ttcgacactt tgtgcaggcc
     4561 cgagaagccg atgtccttcg agcccgtcga gctaccggaa ccgacgtcgg cgttcgcggt
     4621 cggtgcgaag gcacgcggcg aggaggagaa ggtcttcgac gcgatcagac gtatcgtgga
     4681 tgaggatccc tcactcaagc tggagcgctc cgaggcgacc ggtgaggata ttctcgccgg
     4741 gcttgcccag atgcacgtcg agctcgccct ggaacgcatc gagcaccgct acggggtcga
     4801 ggtcgaggcg aggccgccga aggtgccttt tatggagacg atcacgggct ccgcccaggc
     4861 ccagggccgc tacaagaagc agaccggcgg caggggacaa ttcggggacg ccaggatcga
     4921 gctctccccg ttgccgcgcg gttccggctt cgagttcgag aacgccatcg tcggcggcgc
     4981 catcccgcgc cagttcatcc ccgctgtaga gaaggggatc atcgaggcga tggcaggagg
     5041 gacgatcgca ggctaccccg tcgttgacgt gaaggtgcgc ctccacgacg gcgccttcca
     5101 caccgtcgac tcctccgaga tggcgttcaa ggtcgccgga tcaatggcct tcaagaacgc
     5161 tatcgagaag gcccatcccg tcctcctgga gccgtacgtt aaggtcgagg tactcgcgcc
     5221 ctcggagatg gtaggcgaca tcatgggcga cctctctggc agacgcgggc gccctatggg
     5281 aatcgagcaa cgcggtgagc gccaggtcat acaggccgaa gtgccccaga tcgaaatgct
     5341 cacctacgcc cgcgacctcc gctctataac aggcggacgc gccaacttcc acgtcgagtc
     5401 gggccactac gaggaagtcc ccccgaacct ggtggacaaa gtcctcgccg caaacgagag
     5461 ggaggaagag aaggcggtca attagaaggc ttcaggtctc aggcaacagg tttcaggttt
     5521 tgctgatgcc tgagacctgt tgcctataaa gcctcgctca ccgcgtagcg gggaatagag
     5581 accccgacca gggagtctcg tgtgacgagt tcggctaact cttcctcgtt caggttctcc
     5641 gtgtccggcg ggcgcatggg gaggaccatg tagcggacgt ctgcggtgct gtcgtggacg
     5701 agtacttcga cgccttcggg aggctcgaag ccgaactcgc gcatcacggc gcggggctcc
     5761 ctgacggcgc gcgagcggta gtcgaagctc ttgtaccagt ctggcgggcg tccgaggatg
     5821 gcccgcgggt agcacgagca cagggtgcag acgatcaggt tgtgcacgtg gggcgtgttc
     5881 tccacgacga cgaactcggc ggggtgctcg gggtctatgc caagttcaga ggctgcggac
     5941 ttgccgtcgg agagaagcaa ctctttgaag tcctcgtcca cccatgcgcg ggccacgagc
     6001 ttcgcgccgt tggcggggga gcgggcctcc atgtaagaga tcctgtcctg tacctccggt
     6061 tcggtgagga tgcctttctc gatgagcaac tcctctatgg ccctgattcg cgcggcgtag
     6121 ggactgttgg tcctgaccga gcccgcttct tcgtgtgcgt ggtat
//
LOCUS       DATAIR010000403         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_57942, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000403 DATAIR010000000
VERSION     DATAIR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57942"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VHM69_09820"
     CDS             complement(<1..867)
                     /locus_tag="VHM69_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2740735.1"
                     /translation="MANPDTRPVRAGKAALMADQKTPPGALRRALAYLRSYLGESAGA
                     FFSLLLVSGANLATPVLIGRAIDDGIGPRRLGVIFLIVGALVGLALARGLFTFLQGYL
                     AERASQGVAYDLRDALFERIERLSFSYYDRVQTGQLVTRLTSDVEQIRAFAGSGVVQL
                     ANAVVMIVGTTVLLLYLDWQLALVALAIVPVIVLLLLRFIGRIRPLFREVQQTLGRLN
                     TVLQEDLSGVRVIRAFAREDYETARYRSVNDELLEKNLTTVRVFSNNFPFVFLFANLG
                     TLAIIWFGGWQVI"
     gene            complement(956..>2501)
                     /gene="hutH"
                     /locus_tag="VHM69_09825"
     CDS             complement(956..>2501)
                     /gene="hutH"
                     /locus_tag="VHM69_09825"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX2740736.1"
                     /translation="PDVRMTSAVVYAEDLDPASVVAVARRGARVALGREARERVSASR
                     GHVERMIDDAHPVYGVTTGFGALANTRIPAEKRLELQQSLLRSHAAGMGPFVEAEVVR
                     AMMAIRAKTLAAGYSGVRPELIDALISLLNADLAPAVPEHGSMGASGDLAPLAHVGLC
                     LTGDGWMLERGEAVPARESLERAGLQPLPPATKEGLALINGTDGMLAMLLLALEDLKV
                     LLETADVTAAMSVEALLGTDRVFREELHSLRPHPGQLASAGNIARLLRDSPIVASHRD
                     SPHLVQDAYSLRCAPQVYGAARDTMEHAALVASRELDSITDNPVVLPDGRVESVGNFH
                     GEPLAFALDFLAIAASEVGAITERRIDRMLDPKRSEGLPAFLSPLPGVNSGFMIPHYT
                     AAALAEENRRLANPASTGSLPTSAMQEDHNSMGWGAGRKLRLLLGNVARILAVEAACA
                     AQALDLRAPLKPGPATSAVLARIRQDIPFIERDDFMAPHLRTAEELVTTGTLARAAED
                     VLGTLQ"
BASE COUNT          427 a          777 c          877 g          420 t
ORIGIN      
        1 tataacctgc cagccgccga accagatgat cgcaagcgtc ccgaggttgg cgaagaggaa
       61 gacgaaaggg aaattgttcg agaagaccct aacggtcgtg aggttcttct ccagtaactc
      121 gtcgttgacg gacctgtagc gggccgtctc gtagtcctcg cgggcgaagg cacggatcac
      181 gcgcaccccg gagaggtctt cctgcagcac ggtgttcagc cgcccgagcg tctgctggac
      241 ctccctgaag aggggcctta tcctgccgat aaaccgcaga agcaggagga cgatgactgg
      301 cactatggcg agcgcgacga gggcgagttg ccagtcgagg tagagtagta ggaccgtcgt
      361 ccccacaatc atgacgacgg cgttggcgag ctggactacg ccggagccgg cgaaagcgcg
      421 gatctgctcg acgtcgctgg tcaatctggt gacgagctgg cccgtctgga ccctgtcgta
      481 gtaggagaag gagagccgct ctatcctctc gaagagcgcg tcccgcaggt catacgcgac
      541 cccctgcgaa gccctctcgg ccaggtaacc ctgcaagaac gtgaaaagcc cccgcgcgag
      601 cgcgagcccg acgagcgccc cgactattag gaagatgacc ccgagccgcc gcggaccgat
      661 accgtcgtcg atggcgcgtc cgatcaggac cggcgtcgcc aggttcgccc ctgacaccag
      721 gaggagcgag aaaaacgcgc ccgcgctctc cccaaggtac gagcgcagat aggccagcgc
      781 ccttcgtagc gctcccgggg gtgtcttctg gtcggccata agtgcggcct tacccgcccg
      841 tacgggtcgt gtgtccgggt ttgccaagga tcacctgtcc tccttgtcgg ggacggtgcc
      901 tttgcggcgc ggtcccatgc taatggtagc ccatcggggc tataggacgg tacctttatt
      961 gaagagtacc gaggacatct tcggcagccc gcgcgagagt tcctgtcgtc accagctcct
     1021 ccgcagtcct caggtgaggg gccatgaagt catcgcgttc tataaacggt atgtcctgcc
     1081 tgatgcgggc gaggacggcg ctggttgccg ggcctggttt cagaggagcc ctcaggtcga
     1141 gagcctgtgc cgcgcaggcc gcttcgacgg cgaggatgcg ggcgacgttg ccgaggagca
     1201 ggcgcagttt gcggcccgcg ccccagccca tcgagttgtg gtcctcctgc atcgctgaag
     1261 ttgggaggga gcctgtactg gcagggttcg cgaggcggcg gttctcttct gcgagggcag
     1321 cggctgtgta gtgcgggatc atgaaccccg agttgacgcc gggcagcggg ctgaggaacg
     1381 cgggcaggcc ctccgagcgc ttcgggtcga gcatacggtc gatcctgcgc tccgtgatgg
     1441 cgccgacctc ggaggcggcg atggcgagga agtcgagtgc gaaggcgagc ggctccccgt
     1501 ggaagttccc cacgctctcg acccttccgt ccggcagaac gaccgggttg tccgtgatgg
     1561 agtcgagctc gcgactggct accagcgctg cgtgctccat ggtgtcgcgg gcggcgccgt
     1621 agacctgggg ggcgcaacgc agggagtatg cgtcctggac gaggtgggga gagtcgcggt
     1681 gggaggctac gatcgggcta tcccggagca acctcgcgat gtttccggcg ctcgctagct
     1741 ggccggggtg aggtcgcagc gagtggagtt cctcgcggaa gacgcggtcg gtgccgagca
     1801 gagcctcgac ggacatcgcc gccgtcacgt ccgcggtctc gagaagaacc ttcaaatctt
     1861 ccagggcaag aagcagcatc gcgagcatcc cgtcggtgcc gttgatgagg gcgaggccct
     1921 ctttggtggc gggcggaagt ggctggagac cggcgcgctc cagggactcg cgggcgggga
     1981 cggcttctcc gcgttcgagc atccagccat cacccgtcag gcagaggccg acgtgggcga
     2041 ggggggcgag gtcgccactc gcgcccatcg agccgtgttc ggggactgct ggtgcgaggt
     2101 cggcgttgag aagtgagatc aaggcgtcta tcaactccgg gcggacgccg gagtagccgg
     2161 ccgcgagcgt cttcgccctg atagccatca tggccctcac gacctctgcc tcgacaaatg
     2221 gtcccatgcc cgcggcgtgg ctgcggagga gcgattgctg gagctccaga cgcttctctg
     2281 ccgggatcct ggtgttcgcc agcgccccga agccggtcgt caccccgtag acgggatggg
     2341 cgtcgtcgat catgcgctcg acgtgaccgc gcgaggcgct gacgcgctcg cgggcctccc
     2401 tgccaagcgc cacccgcgct ccacgcctgg cgaccgcgac gacggaggcc gggtccagat
     2461 cctctgcgta gactactgcg gaagtcatcc tgacatcagg t
//
LOCUS       DATAIR010000404         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_303092, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000404 DATAIR010000000
VERSION     DATAIR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303092"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHM69_09830"
     CDS             complement(<1..309)
                     /locus_tag="VHM69_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2740737.1"
                     /translation="MPEAGSLITNPREYRRTAPEPVRHTPTVYHIPVCPFSQRLEILL
                     TLKGCRHLVDFHVVDITRPRPGWLIEKMHGTTALPVLETEEGGIIKESMVILRYLEDR
                     Y"
     gene            complement(441..1442)
                     /locus_tag="VHM69_09835"
     CDS             complement(441..1442)
                     /locus_tag="VHM69_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2740738.1"
                     /translation="MSVRVGIVSFAHVHSPPYAAALRDLDIADFVGITDEDAVRGAEA
                     AESFGVRFFESADSLFDEVDAVVVCSENRNHARDVIPALQSGVHVLCEKPIATTVEDA
                     RAMIRASETSGSQLRTAFPVRYLSSVVRAREIVRGGSLGRVLALNGTNHGQIPGGWFI
                     DPELAGGGAVMDHTVHLADVLRWMLDAEVRSVYAEVDSFFGAEGTDDAAILTLELEGG
                     PIADGTFATIDPSWSRGDGFPTWGDVTLRISGTSGVLDVDPFARPLKTFDHETRAPVW
                     SYTGEDMNALMLADFLRGVAEDDPAGASGLDGLRTLEIVLAAYRSGVDHEPKIISRT"
     gene            complement(1439..2416)
                     /locus_tag="VHM69_09840"
     CDS             complement(1439..2416)
                     /locus_tag="VHM69_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2740739.1"
                     /translation="MRVGLVGAGFMGGVHLNAYAAIPEVEVVGVADARIESAEAGAAI
                     VGARPYGSYEELVAAEDVEVVDVCLPTVFHRDIAVRAAAEGRHVILEKPISRTIEDAQ
                     EILDAFSGEGPRLFVGHVVRFFPEYVGIKEKIESGDLGTVGVVRTSRRSPLLFGWNDW
                     YADWRISGGVLLDLVIHDFDFLRWALGEVERVYARGVLGHEYNRLDYALTTLRFESGA
                     IAHVEGHWGFPGLFNYSIEVAGSRALLTADSREPASLELIGGATDDVVDLATGKSPYE
                     AELAHFVRCISTGEEAIVEGRDAYEALRIGLAATESVLTGKPVTLGGRG"
     gene            complement(2469..3788)
                     /locus_tag="VHM69_09845"
     CDS             complement(2469..3788)
                     /locus_tag="VHM69_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2740740.1"
                     /translation="MMHARRLSRRDFIKVGGAGLAGAALLGAAGCGGGEQGGGATKIY
                     FTSAPDESGTTEKLIDMFNDKNKGEYRVIFRQGNADTGQRFDKLRTEMQAGGEDIDVI
                     LGDVIWTAQLAENGWISDLSDRFTESQRKEFLPGSVEAITYKGKTFGMPWFTDTGLLY
                     YRKDLLQESGFDGPPKTWDELKQMTKQVKEDAGLKYGFVFQGAAYEGGVCDGMEFIWT
                     HGGEVLDPNDPTKVLVDSPEAIAGLATERSMITEGISPEGVTVYKEDESAGAFLNGDA
                     VFLRNWPYVYALVGTSEYPELEPEQVGVSELPSADGEPGNGTVGDQPLYISSTATDPD
                     AAWKFIQFLTAPEQQKIRAVEGAYLPTRTALYEDPEIQEKVPVVPLARVALQHTRPRP
                     VSPYYSDMSLEMAEQFNASLSGEITPEEAARTLKEQLERFIEEGQET"
     gene            complement(3808..4602)
                     /gene="iolB"
                     /locus_tag="VHM69_09850"
     CDS             complement(3808..4602)
                     /gene="iolB"
                     /locus_tag="VHM69_09850"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX2740741.1"
                     /translation="MPDLLVSSNHQADGVVVRVTPESAGWEYVGFEVLRLEAGRSVSR
                     HTGAEEVCIVPLSGTCTVSAGGEEWNEIGGRKNVFDGPPFALYLPPGTDYSIEAVTDL
                     ELAVASAPAERGVEPMLVRPEDIEVEKRGSGNMEREIRPILMEDRPAERLLVVEVLTP
                     NGHWSSYPPHKHDEDDPPRERYLEETYYHRLRPERGFALQRVYTEDGSLDETLSFHDG
                     DVVLVPKGYHVVSSPPGYDLYYLNVMAGPVREWKIKNHPDHEWLLG"
     gene            complement(4619..>5168)
                     /locus_tag="VHM69_09855"
     CDS             complement(4619..>5168)
                     /locus_tag="VHM69_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2740742.1"
                     /translation="GYEISGGLGVKMADPDREVYVMCGDGSYLMMNAEIVTSIQEGYK
                     LTMVLVDNSGFSSIGALSRSVGSDGFGTQYRFRKDGSIGLDSEEDPGDILPVDLATNA
                     ETLGAHVIRTQSVEDLKKALGDAKGVDRTVVIHIPVDRYEGVPDYDSFWDVPVAEVSE
                     MESVISARKEYAKNRESERRYL"
BASE COUNT          884 a         1714 c         1559 g         1011 t
ORIGIN      
        1 gtagcggtct tccaggtacc tgaggatgac catgctctcc ttgatgatcc cgccttcctc
       61 agtctcgagc acaggcagag ccgtcgtgcc atgcatcttc tcgatcagcc accccggccg
      121 cgggcgcgtg atatcaacga cgtggaagtc gacgaggtga cgacaaccct tgagggtgag
      181 caggatctcc aaccgctggg agaatggaca gacgggtatg tgatagacgg tcggcgtgtg
      241 ccgcaccggc tcgggagcgg tccggcgata ctcccgcggg ttagttatca ggcttcctgc
      301 ttcgggcatc gtgccatcct tccaatgttg tatcgagacc agcgcgtggt acccgcgccc
      361 gccgcaaccg tatcctacga acgggccatt tctcggaaac gccggtgacc ggcgtgtcgt
      421 gcctgagtag gcgaaaccgt ctacgttcga cttatgatct tgggttcgtg gtccactccc
      481 gagcggtatg cggccaggac tatttcaagg gtccgcaatc catccagccc gctcgctccg
      541 gcagggtcgt cttctgcgac gccgcgcagg aagtccgcga gcatcagggc gttcatgtcc
      601 tctcccgtgt acgaccacac cggagcgcgt gtctcatggt cgaaggtctt caagggccgc
      661 gcgaacgggt ccacgtcgag gactcccgag gtgccagaga tcctgagtgt cacgtcgccc
      721 caggttggga agccatctcc gcgcgaccag ctcgggtcta tggtggcgaa ggtgccgtcg
      781 gcgatggggc cgccttcgag ctcaagggtc aggatggccg cgtcgtccgt cccctctgcc
      841 ccgaagaacg aatccacctc cgcgtagacg ctccgcacct cggcgtccag catccagcgt
      901 agaacgtccg cgaggtggac cgtatggtcc attacggcgc cgccgccggc cagttcggga
      961 tcgatgaacc agccgccggg gatctggccg tggttcgtgc cgttgagggc cagaacgcgt
     1021 ccaagagagc ccccgcgcac gatctcccgc gccctcacga ccgaagagag gtagcgcacg
     1081 gggaatgcgg tcctgagctg gctgcccgag gtctctgagg cccggatcat ggcccgcgcg
     1141 tcttcgaccg tggtcgcgat gggcttctcg cagagaacgt gtacgccgct ctggagagca
     1201 gggatcacat ccctcgcgtg attcctgttc tcggagcaga cgacaacggc atcgacctca
     1261 tcgaacaggg agtccgcgct ctcgaagaaa cgcactccga aactctccgc ggcctcagcc
     1321 ccgcgaactg catcttcgtc agtgattccg acgaaatccg cgatgtcgag gtccctgagc
     1381 gctgccgcat agggtggaga gtggacgtgg gcgaagctga cgatgccgac tctgacgctc
     1441 atccgcgacc tcccagcgta acgggcttgc cagtcaggac cgattctgtg gcggcgaggc
     1501 cgatcctgag cgcctcgtaa gcgtcccgcc cttccacgat cgcctcttct cccgtactga
     1561 tgcaacggac gaagtgtgca agctccgctt cataggggct cttgcctgtg gcgaggtcaa
     1621 caacgtcgtc ggtcgctccg ccgatcaact cgagcgaggc cggctccctg ctgtccgccg
     1681 tgaggagggc gcggctgcct gccacctcga tggagtagtt aaagaggccg gggaaccccc
     1741 agtggccctc gacgtgggcg atggcgccac tctcgaagcg cagggttgtg agggcgtagt
     1801 ccaggcggtt gtactcgtgt ccgagcacgc ctctagcgta aacccgctcg acctcgccaa
     1861 gggcccagcg caggaaatcg aagtcgtgga tcacaaggtc gagcagtaca ccgccgctga
     1921 tgcgccagtc ggcgtaccaa tcgttccagc caaagagtaa gggagagcgg cggctggtgc
     1981 gaaccacgcc gacggtaccg aggtctccgg actcgatctt ctccttgatc ccgacgtact
     2041 cggggaagaa acggacgacg tggccgacga agagtcgagg cccttcgcct gagaatgcgt
     2101 ccaggatctc ctgcgcgtct tcgatggtgc gggatatggg tttctcgagg atcacgtgcc
     2161 tgccttcggc cgccgccctg accgcgatgt ctctgtgaaa gaccgtcggc aggcagacgt
     2221 ccaccacctc gacgtcctcc gccgcgacga gctcctcgta cgatccatat ggccgcgccc
     2281 cgacgatcgc cgcgccagct tccgcgctct cgatcctggc gtcggcgacg ccgacgacct
     2341 ccacctcggg gatggcggcg taggcgttca gatgcacacc gcccatgaag ccggcgccta
     2401 ccagaccaac ccgcatctat cccccctctt cgtcgcgttc aagaatcgtc tcccatcgtg
     2461 ccgggttcct atgtttcctg gccctcctct ataaagcgct ccaactgttc cttgagggtc
     2521 cgcgccgctt cttcgggcgt tatttcgccg gagagcgagg cgttgaactg ctcggccatc
     2581 tccagggaca tgtccgaata gtacggggat accgggcgcg gacgggtgtg ctggagcgcc
     2641 actctcgcga gcggtacaac ggggactttc tcctgtatct ccgggtcttc gtagagcgct
     2701 gtccgggtcg gcaggtacgc gccttcgaca gcccgaatct tctgttgttc gggggccgtg
     2761 aggaactgta tgaacttcca ggcggcgtcg gggtccgtcg ccgtggagct gatgtacagg
     2821 ggctggtccc cgaccgtgcc gttgcctggt tctccatcag ccgacggcag ttcggagacc
     2881 ccgacctgct ccggctccag ctccgggtat tccgacgtgc cgaccagggc gtacacgtag
     2941 ggccagttac gcaggaagac cgcatcaccg ttcaggaacg cgccagcgga ctcgtcctcc
     3001 ttgtataccg tgactccctc gggggatata ccctcggtga tcatgctgcg ttccgtggcg
     3061 aggccagcta tggcctccgg gctgtctacg aggaccttgg tggggtcatt cgggtccaaa
     3121 acctccccgc cgtgggtcca gatgaactcc atgccgtcgc aaacgccgcc ctcgtacgca
     3181 gccccctgga agacgaagcc gtacttgagg cccgcatcct cctttacctg cttggtcatc
     3241 tgcttgagct cgtcccaggt cttcggagga ccatcgaacc cgctctcctg cagcagatcc
     3301 ttgcggtaat agaggagtcc cgtatccgtg aaccacggca taccgaaggt cttgcctttg
     3361 taggttattg cctcgacgga gccgggcaga aactcctttc gctggctctc cgtaaatcgg
     3421 tctgagaggt cggagatcca accgttctcc gccagctggg cggtccagat gacgtccccg
     3481 aggatgacgt ctatatcctc ccccccggct tgcatctcgg tgcggagctt gtcgaagcgc
     3541 tgccccgtgt ccgcattgcc ctggcggaag atgaccctgt actcgccctt gttcttgtcg
     3601 ttgaacatgt cgatgagctt ctcggtggtc cccgattcgt cgggcgctga ggtaaagtag
     3661 atcttcgtgg cgcccccgcc ctgctcgcct ccgccgcaac ctgctgctcc gagtaaggcc
     3721 gcccccgcga gtcctgcccc gcccaccttg atgaagtccc tgcgtgagag tcgtcttgcg
     3781 tgcatcattc gcgtctccct tcgaccgcta tccgagcagc cactcgtggt cggggtggtt
     3841 ctttatcttc cactcgcgca cgggcccggc catcacgttg aggtagtaga ggtcgtagcc
     3901 gggcggcgac gagacgacgt ggtagccctt cggcacgagc accacgtcac cgtcgtggaa
     3961 cgacagcgtc tcgtcgagcg agccgtcttc ggtatatacc ctctgcaacg cgaagcctct
     4021 ctccggcctg agcctgtgat agtacgtctc ctccagatat cgctcgcgcg ggggatcgtc
     4081 ctcgtcgtgc ttgtgcggag ggtagctcga ccagtgaccg ttcggggtaa gtacctcgac
     4141 cacaaggagc cgctccgcag ggcggtcctc catcaggatc ggacgtatct cccgctccat
     4201 gttgcccgag ccgcgtttct cgacctcgat gtcctcgggc ctgaccagca tcggctcgac
     4261 cccccgttcc gcgggcgccg aggcgaccgc taactcaagg tctgtcacgg cttcgatgct
     4321 gtaatccgta cctggcggca ggtagagcgc gaagggagga ccgtcgaaga cattcttgcg
     4381 gccaccgatc tcattccact cttcaccacc cgctgacacc gtacacgtcc cggagagagg
     4441 gacgatgcag acctcctcag cgcctgtatg ccggctcacg ctccggccag cctccagccg
     4501 cagcacctcg aagcccacgt attcccagcc cgcggactcc ggcgtaacac gtactacgac
     4561 gccatcagcc tgatgattgc tgctcacgag caaatctggc acgctctgaa gctccttcct
     4621 aaagatatct gcgttctgac tctctgttct tcgcgtactc cttgcgggct gagatcactg
     4681 actccatctc cgagacctcg gccacgggca cgtcccagaa cgaatcgtag tcaggcacgc
     4741 cctcgtagcg gtctacgggg atgtggatca caaccgtgcg gtcaacgcct ttggcgtccc
     4801 cgagggcctt cttgagatcc tcgacgctct gtgtccggat cacgtgcgcc ccgagggtct
     4861 cggcgttagt ggcgagatcc accggcagta tgtcgccggg atcttcctcg gagtcgaggc
     4921 cgatggaacc gtccttgcgg aaccgatact gggtaccgaa gccatccgag ccgacggacc
     4981 gggacaacgc tccgatcgag gagaagccgg agttgtccac gaggaccatc gtcagcttgt
     5041 agccttcctg gatagatgtg actatctcgg cgttcatcat caggtacgaa ccgtcgccgc
     5101 acatgacgta aacctcgcgg tcggggtctg ccatcttgac gccgaggccg ccggagatct
     5161 catacccc
//
LOCUS       DATAIR010000405         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_334557, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000405 DATAIR010000000
VERSION     DATAIR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334557"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VHM69_09860"
     CDS             complement(<1..951)
                     /locus_tag="VHM69_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2740743.1"
                     /translation="MTKTKRYRRNTVARVLVFTVVLLATASGAYMVGRSQSPASLDEQ
                     NRKSLALYAEALDAVQDDYVDQGAVDTKKQTYGAIEGMLKTLGDEGHTRFLTPEERKQ
                     NTKSLSGVYEGIGIEIESKGDKVVVVAPIEGSPADRAGFEPGDVLISVNGKSVRGAEI
                     SEVVNKVRGPQGTDVEVVVLRGQQERTFDLQRDEIKSPAVSWAMIPETDIAYVLLSSF
                     TDDSAGDLREAFDKARAAGARRFVLDLRNNPGGRLDQTVQMAGYFLEPGSVIYIRKDA
                     SDEREEIKVEGDPESTDAPLVVLVNEGSASSSEILAGALRD"
     gene            complement(1020..>1865)
                     /locus_tag="VHM69_09865"
     CDS             complement(1020..>1865)
                     /locus_tag="VHM69_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2740744.1"
                     /translation="LLEPTEGEVLFEGEDILGYDRKQMLVVRRNMQIVFQDPYASLNP
                     RMTVGNIVSEPLQIHEVGGSSAGERKKRVQELLEVVGLNPEHYNRYPHEFSGGQRQRI
                     GVARAIALNPRLIICDEPVSALDVSIQAQVINLLQDLQKEFDLTYVFIAHDLSVVKHI
                     SDRVAVMYLGKIVEIADRATLFGKPRHPYTASLLSAIPIPDPQKERERQRVVLAGDVP
                     SPANPPSGCHFHTRCPRAQDYCKEHEPGLDPQENESHGAACFFPVLEGQRIEESATST
                     PYRAE"
BASE COUNT          305 a          595 c          575 g          390 t
ORIGIN      
        1 gtccctcagg gctccagcca gtatctcgga gctcgaggcc gacccctcat tcacaagaac
       61 cacgagcggc gcgtccgtcg attccgggtc gccttcgact ttgatctcct cgcgctcgtc
      121 cgaagcgtcc ttgcggatgt aaataacgct ccccggttcg aggaagtacc cggccatctg
      181 taccgtctga tccagccttc caccagggtt gttcctgaga tcgaggacaa agcgcctggc
      241 gcccgccgcc cgcgccttgt cgaaggcctc cctgagatcg cccgcgctgt catccgtgaa
      301 cgacgagagg agaacgtaag ctatgtcggt ctcagggatc attgcccagg aaaccgccgg
      361 ggacttgatc tcgtcgcgct gtagatcgaa cgtccgctcc tgctgcccgc gcaagacgac
      421 tacctcgaca tctgtaccct gtgggccacg caccttgttg acgacctccg agatctcagc
      481 gccccgcacg ctcttcccgt tcacggagat caggacatca cctggctcga aaccggctct
      541 gtcagcagga gatccctcta tgggtgcaac gactacgacc ttgtcgccct tgctttcgat
      601 ctcgatcccg atcccctcgt agacgccgga gaggctcttg gtattctgct tgcgctcctc
      661 gggcgtcaga aaacgcgtgt gcccctcgtc tccgagggtc ttgagcattc cttctatggc
      721 cccgtatgtc tgcttcttgg tgtccacggc cccctggtcc acgtaatcgt cctgcaccgc
      781 gtcgagcgcc tcggcgtaga gggccaggct cttgcggttc tgttcgtcca gtgaagcggg
      841 gctctgcgaa cgtccgacca tgtatgcccc tgacgcggtc gcgagcagca ccacggtgaa
      901 gaccagcacc cgcgcaacgg tgttccgccg ataacgtttg gtcttggtca ccttccacac
      961 gcggcaattg tactatctca gcgtgtcagc acggcgtcga agccggagcg cgctgtaagc
     1021 tattcggccc gatacggcgt gctggttgcg gactcctcta tacgctggcc ttcgagaacg
     1081 gggaagaagc aggcggcgcc gtggctctca ttctcctgcg ggtcgaggcc gggttcgtgc
     1141 tccttgcagt agtcctgtgc gcgcgggcaa cgggtgtgga agtggcatcc gcttggaggg
     1201 ttcgcggggg atggtacgtc tccggcgagt accacgcgct ggcgctcgcg ctccttctgg
     1261 gggtctggta tcggaatggc cgaaaggagc gacgcggtgt aggggtggcg cggcttgccg
     1321 aagagcgtcg ccctgtcggc gatctccacg atcttgccaa ggtacatcac ggccaccctg
     1381 tccgagatgt gcttgaccac ggagaggtca tgggcgataa agacgtaggt gaggtcgaac
     1441 tccttctgga ggtcctgcag caggttgatg acctgagcct ggatcgagac gtccagagcg
     1501 cttaccggct cgtcgcagat gatcaggcgc gggttgagtg cgatagcacg ggccaccccg
     1561 atcctctgcc tctgtcctcc cgagaactcg tgcgggtagc ggttgtaatg ctcggggttg
     1621 aggccgacca cttcgagaag ctcctgcacc cgcttcttgc gctcgcctgc ggagctgccg
     1681 ccgacttcgt ggatctgcag tggctccgag acgatgttgc ccaccgtcat gcgggggttc
     1741 aggctcgcgt aggggtcctg gaagacgatc tgcatattcc tgcgcacgac gagcatctgc
     1801 ttgcggtcgt agccgagtat gtcctcgccc tcgaagagga cctcgccctc cgtgggctca
     1861 agtag
//
LOCUS       DATAIR010000406         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_150680, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000406 DATAIR010000000
VERSION     DATAIR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150680"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /gene="cofG"
                     /locus_tag="VHM69_09870"
     CDS             complement(<1..2006)
                     /gene="cofG"
                     /locus_tag="VHM69_09870"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX2740745.1"
                     /translation="MEEAAEVRDRAFGPRISYSRKVFIPLTKLCRDNCGYCTFAHPPR
                     PGERAYLTPEEVLQIARAGADAGCKEALFTLGDKPEKRYPEARRELQDLGFGSTVEYL
                     IHVCRLVVEETGLLPHANPGVLAEEEVRDLRHVAVSQGIMLEQASDRLLGPNTAHWAS
                     PDKVPAKRLKTIDAAGRLGVPFTTGILIGIGETIEERVDTLLAIRDAHRRHGHVHECI
                     VQNFRAKPGTRMALSPEPSENEMLATIALARLILPPDMTVQAPPNLAEVGQDGTPPYA
                     SYIDAGINDWGGVSPITPDHVNPERPWPHLEDLEKATESKGYLLLERLALHPSYAREA
                     ETWVDEKLRSGVWAGMDAEGFARVEAWSPGTLEEIPSSTVAESLGRRPSKVRPEFARA
                     LAVAGERDLDEAEISILFTARGTELAELCRVADEIRREVNGDEVTYVVNRNINYTNQC
                     YFRCRFCAFSKGPKSLNLRGEPYLLDTTEVARRAREAWGKGATEVCMQGGIHGTFTGK
                     SYIEYLRAVKEEVPEMHVHAFTPLEVSQGARTLGLSVEEFLEELKEAGLGTLPGTAAE
                     ILDDEIRAIICPDKIDTAGWSDVMRAAHAIGLRSTTTIMFGHVEGPVNWARHLLVLRE
                     IQAETGGFTEFVPLPFVHMGAPLFLQGRSRKGPTFAEALKMHAVG"
BASE COUNT          329 a          702 c          652 g          392 t
ORIGIN      
        1 cccacagcgt gcatcttgag cgcctcggcg aaggtcgggc ccttgcgaga gcggccctgc
       61 aaaaatagcg gcgctcccat atggacgaaa ggcagcggca cgaactcggt gaagccgcct
      121 gtctcggcct gtatctcgcg cagaacaagc aggtgccgcg cccagttgac gggaccttcc
      181 acgtggccga acatgatcgt cgtggtcgag cgcaggccga tggcgtgagc ggcacgcatg
      241 acgtcggacc atcccgcggt gtcgatcttg tccggacaga tgatggccct gatctcatcg
      301 tccagtatct ccgcagccgt ccccggcaac gtccccaacc ccgcctcctt gagctcctcc
      361 aggaactcct cgacggagag cccgagcgtc cgggcaccct gcgagacctc tagcggcgtg
      421 aaagcgtgga cgtgcatctc aggcacttct tccttgacgg cccgcaggta ctcgatatag
      481 ctcttgccgg tgaacgttcc gtggatgcca ccctgcatgc agacctctgt ggcgcctttc
      541 ccccaggcct cgcgggcgcg tcgcgcgacc tcggtcgtgt ccagaaggta cggctcgcct
      601 cgcaggttca gggacttcgg acccttggag aaggcgcaga agcggcacct gaagtagcac
      661 tggttcgtgt agttgatgtt cctgttgacg acgtaggtaa cctcgtcgcc gttcacttcg
      721 cgtcttatct cgtctgcgac gcggcacaac tcggcgagct ccgtgccgcg cgccgtgaag
      781 agaatggaga tctccgcctc gtcgaggtca cgctcgccgg cgacggccaa cgcccgcgcg
      841 aactcgggac gtaccttcga gggacgcctc cccagcgact cggcgacggt actggagggg
      901 atctcctcca gggttcctgg cgaccatgcc tccacccgcg cgaaaccctc tgcatccatg
      961 cctgcccaca cacccgaacg gagcttctcg tccacccagg tctccgcctc gcgggcgtaa
     1021 gacggatgaa gggcgagccg ttcgaggagc aggtagcctt tgctttcggt ggccttctcc
     1081 agatcttcca gatgcggcca gggccgctcc gggttgacgt ggtcgggggt gatgggggag
     1141 acgcctcccc agtcgttgat gcccgcgtcg atgtaactgg cgtatggggg tgtcccgtcc
     1201 tgtccgacct ccgccaggtt tggcggggcc tgcaccgtca tgtccggcgg taggatcagg
     1261 cgcgccagcg cgatggtcgc gagcatctcg ttctccgagg gttccggtga gagggccata
     1321 cgcgtgcctg gttttgccct gaagttctgg acgatgcact cgtgtacgtg accgtgccgc
     1381 ctgtgtgcat ccctgatcgc gagtagggtg tcgacccgct cttcgatcgt ctcgccgatc
     1441 ccgatcagga tccccgtcgt gaagggcacc ccgagcctgc ctgcggcgtc tatagtcttg
     1501 agacgcttgg cgggcacctt gtctggtgag gcccagtggg ccgtgttcgg cccgagcagc
     1561 ctgtccgagg cctgctcgag catgatcccc tgcgagacgg cgacgtgcct gagatccctg
     1621 acctcctctt cagcgagaac ccccggattg gcgtgcggca acaatcccgt ttcctcaacg
     1681 accagccggc acacgtggat gaggtactcg acggtgctcc cgaatcccag gtcctgcaac
     1741 tcgcgccgcg cttcggggta gcgcttctcc ggcttgtccc cgagcgtgaa gagcgcctcc
     1801 ttgcagccag catccgcgcc cgcgcgggcg atctgaagca cttcctccgg cgtcagatag
     1861 gcccgctccc cgggacgcgg cgggtgggcg aaggtgcagt aaccgcagtt gtccctgcac
     1921 agcttggtga gcggaatgaa gactttgcgc gagtagctga tgcgcggacc gaaagccctg
     1981 tcgcggacct ccgccgcctc ctccatgaca gaggtcggat cctcgtacgc ggatctcgcc
     2041 agctcgtaaa attcctcccg tttcgccccc ccgga
//
LOCUS       DATAIR010000407         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_441237, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000407 DATAIR010000000
VERSION     DATAIR010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441237"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VHM69_09875"
     CDS             <1..1378
                     /locus_tag="VHM69_09875"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX2740746.1"
                     /translation="CEEVLGVADAGVERSLRFAADEVVPRLMRTPEEMENLLSGLRGG
                     YVPAGPSGSPTRGLVNVLPTGRNFYSVDPKALPSRLSWEVGQGLADDLLSRYLEEEGR
                     YPESVGIVVWGTAAMRTQGDDVAEILALLGVRPVWNEESRRVTGIEILPLDELGRPRI
                     DVTVRISGFFRDAFPNLISLMDDAFTTVAGLDEPEDMNFVKKHVVEEKSNGADDRRAT
                     TRIFGSKPGAYGAGLLPLMDARNWRTDEDLAEVYAVWGGYAYGKGLDGVEAREAMESN
                     LRRTEVAVKNIDNREHDLFDSDDYFQYHGGMIAAVRALTGRDPKAYIGDSADPSRVKT
                     RDLSEEARRVFRSRVANPKWIEAMQRHGYKGAFELSATVDYLFGYDATAGVVEDWMYR
                     DVTRKYVLDQEVRDFMQQSNPWALRAISERLLEAAERGLWSEPEEDDLQALKRAYLEN
                     EGMLEEQR"
     gene            1479..3407
                     /locus_tag="VHM69_09880"
     CDS             1479..3407
                     /locus_tag="VHM69_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HEX2740747.1"
                     /translation="MSAPFPFSAIVGQEDLKLALLVNAVSPEVGGVLVRGEKGTAKST
                     AVRALARLLPQIWIVSGCPYSCDPASPNPECPAGPHPPDAPSETRPVRLVELPVGAST
                     DRLAGTLDIEKALSEGRKAFEPGLLAAAHRGILYVDEVNLLSDHLVDLLLDVAAMGVN
                     YVEREGVSVRHPSRFILVGTMNPEEGELRPQLLDRFGVTVEVSGSPDPADRVEVVRRR
                     LRYEDDPEEFAGEWSAMDGELAASVESARMRLPTVDLDEEILFRISTLCADLGVDGLR
                     GDLVTAKTARTLAAWDARDDVLLDDVKRAALLALSHRRRRGPFEQPGIDPDELENALS
                     EDPDPPDGPDGGAPPRGESGDDGEASHSARDPQGRTGAGERSHTATEPFKPVRLEAPE
                     KGRGGPLGRRSRVSGESGHPVGDRETGSSNDVALAATIRAAAPHQMARGREGPGIELR
                     PCDLRQNVREGREGNLILFLVDASGSMAARKRMSAVKGAVLSLLNDAYQRRDKVALIS
                     FRNEDARILLPPTSSVELAASRLEELPTGGRTPLAAGLEKAAEVLKREKLRDRERRPL
                     LVLLTDGRATAGPDPLTAAARLRSLVATSFVVDTEEGYVRLGMAGEIAEAMEARCLRL
                     EELRADSLVDLIERRRVA"
     gene            3404..3985
                     /gene="cobO"
                     /locus_tag="VHM69_09885"
     CDS             3404..3985
                     /gene="cobO"
                     /locus_tag="VHM69_09885"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2740748.1"
                     /translation="MSEVDEAREPRERRLRKRSNRERPLLIVNTGHGKGKSTASFGIM
                     LRGWARGYKIGVYQFVKSGKWKVGEHQAALKLGNIEWFKMGDGWTWTSKDLIESADKA
                     REGWEEVKRRLADETYDMLLLDEFTYPMKFGWVDTNEVVEVLKNRPGFQHVIITGRDA
                     PPELVEISDLVSEVNKVKHPFDEGYRGQKGIEW"
     gene            3979..5340
                     /locus_tag="VHM69_09890"
     CDS             3979..5340
                     /locus_tag="VHM69_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX2740749.1"
                     /translation="MVDTPRIVVAGTHSGVGKTTVASGLMAALSSNGYRVAPFKVGPD
                     FIDPSYHSLATGHPGRNLDAFLSGPALIGPLFAHGALGADIAVIEGVMGLFDGKSGGE
                     LASTAHVAKLLNAPVVLVVDARAMSRSVAALVHGFSTFDLKLNIMGVVLNRVGSSTHE
                     KMLREALKPLGIPVLGALRRDVTIQTPDRHLGLVPVAERREEAGRSLDALGTVISRYL
                     DLDDIVRIARSAEPLKAGPWSPEFSEGQRVDGVRIAVTAGPSFTFMYRENLELMESAG
                     AEVVTFDPTEDEDLPDGTDALYIGGGFPEAYAEILSSNGRMREKVRAFAASRRPIVAE
                     CGGLLYLLRELDGHPMCGVLDATARMTDRLTLGYREARALTVSPLAETGTSVRSHEFH
                     YSSVEPGAGETPAWELAGRGPEGFVSGGVHASYLHTHWAATPELPRRLVRAASRSAVE
                     ATV"
     gene            5337..>6029
                     /gene="cobI"
                     /locus_tag="VHM69_09895"
     CDS             5337..>6029
                     /gene="cobI"
                     /locus_tag="VHM69_09895"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX2740750.1"
                     /translation="MSGRLVGVGVGPGDPELLTIKGLRVLREADEVFVPVADTGEVGR
                     AEATVREYLDEGRIRRLLFALSDDAEARERNWTNAAREVSEHLRGGKTCAFATIGDPN
                     VYSTFTYLARTVRGFVPEVEVETVPGITALQDLASRSGTVLLEGDERLALLPFTAGEE
                     PLREALAGFETVVCYKGGSRLGEVLGVAEEGGRLEGGVYGARLGIEGEEVVAAREMVG
                     REGTYLSTVIFTE"
BASE COUNT         1108 a         1806 c         2089 g         1026 t
ORIGIN      
        1 ctgcgaggaa gtccttggcg tcgctgacgc cggggtggag cgttctttgc ggttcgccgc
       61 cgacgaagtc gtgccgcgtc tgatgcggac gcccgaggag atggagaacc ttctctccgg
      121 cctcaggggc gggtacgtgc ccgccggacc gtctggttct ccgacacgcg ggctcgtgaa
      181 cgtactgccc accggacgca acttctactc cgtcgatccc aaagcgctgc cttccaggct
      241 ctcctgggag gtggggcagg ggctcgctga cgacctgttg agcaggtact tggaagagga
      301 ggggcggtat cccgagagcg tcgggatcgt ggtatggggc acggcggcca tgagaacaca
      361 gggtgacgat gtcgcggaga tactggcact cctcggcgtg aggcccgtct ggaacgaaga
      421 gtcgcgccgg gtgacgggga tcgagatcct tccgctcgac gagctgggac ggccgaggat
      481 cgacgttacc gtcaggatca gtggcttctt cagggacgct ttcccgaatt tgatctccct
      541 gatggacgat gccttcacga ccgtcgccgg cttggatgaa ccggaggata tgaacttcgt
      601 gaagaaacac gtagtcgaag aaaagagtaa cggcgccgac gaccgccgcg ccaccacgcg
      661 catcttcggc tcgaagcctg gcgcttatgg agccggactt ctccctctga tggacgcgcg
      721 caactggcgc acggacgagg accttgccga agtgtatgcg gtatggggcg gctacgccta
      781 cgggaagggt ctggacggcg tcgaggctcg ggaggcgatg gagtccaacc tgcgccgtac
      841 cgaggtcgcg gtaaagaaca ttgacaaccg cgagcacgac ctcttcgact cggacgacta
      901 cttccagtac cacgggggga tgatcgccgc cgtccgcgcc cttacgggcc gcgacccgaa
      961 agcgtacatc ggagactcgg cagacccatc cagggtgaag actcgagacc tctccgaaga
     1021 agcccgccgc gtcttccgct cacgcgtcgc caacccgaag tggatagagg ccatgcaacg
     1081 ccacggctac aagggcgcct tcgagctttc ggccaccgta gactacctct tcggctacga
     1141 tgccaccgca ggcgttgtcg aagactggat gtaccgcgac gtaaccagga agtacgtgct
     1201 tgatcaggaa gtacgggact tcatgcagca gtccaacccc tgggcgctgc gcgccatctc
     1261 cgagcgcctg ctggaggcgg cggaacgtgg cctctggtcc gagcccgaag aagacgacct
     1321 gcaagctctc aagcgggcat acctggagaa cgaggggatg ctggaggagc agcggtgaga
     1381 agcaattctg tgatcctcaa cttcagggta aagtttggag gtctcaccct cttgctattc
     1441 atgagcagca aggtggccat acgagagatg ggtcgtcgat gagcgcaccc ttcccgttct
     1501 cggccatcgt cgggcaggag gatctgaaac tggccctgct cgtgaacgcc gtctcgcccg
     1561 aggtcggggg cgttcttgtg cgcggcgaga aggggaccgc gaagtctacc gcggtacggg
     1621 cgctggctcg tctactgccg caaatatgga ttgtctccgg ttgcccgtac tcttgtgatc
     1681 ctgcgtcccc gaacccggaa tgcccggccg ggccgcaccc gcctgacgcg ccttctgaga
     1741 cgcgtcctgt gcggctggtg gaactacctg tcggggcgag cacggatcgg ctggccggga
     1801 cgctggatat cgagaaggcg ctctccgagg ggaggaaggc gttcgagccg gggttgcttg
     1861 cggcggcgca caggggcatc ctgtacgtgg acgaggtgaa cctgctctcc gaccacctcg
     1921 tggatttact tcttgacgtg gccgcgatgg gcgtgaacta tgtggagcgc gagggcgtga
     1981 gtgttcggca tccttcgcgc ttcatcctgg tcggcacgat gaacccagag gagggcgagc
     2041 tccgcccgca actactggac cgcttcggcg tcaccgtcga ggtctcgggg agcccggatc
     2101 ccgccgacag ggtcgaggtc gtccgtcgca ggctgcgcta cgaggacgat ccggaggagt
     2161 tcgccggaga atggtctgcc atggacggag agctcgccgc gtccgtggaa tcagcacgca
     2221 tgcgtcttcc gacggtggat ctcgacgagg agatcctgtt caggatctcg actctctgcg
     2281 ccgatctggg cgtggacggc ttgcgcggcg acctcgtaac cgcgaagaca gcccgtacgc
     2341 ttgccgcctg ggacgcccgc gacgacgtac tcctcgacga cgtgaagcgg gccgccctgc
     2401 ttgccctctc gcaccgccgc cggcgaggac cattcgagca gccaggcata gaccccgacg
     2461 aactcgagaa cgccctctcc gaagaccccg atccaccaga cggccctgat ggcggcgcgc
     2521 ctccacgtgg cgaaagcggg gacgacggcg aagcctccca tagcgctcgt gatccccagg
     2581 ggcggacggg agcaggtgag cgtagccaca ccgcgacgga gccgttcaaa cccgtgcggc
     2641 tggaggctcc cgagaaaggc aggggagggc ctctcgggcg acggagcagg gtttcgggtg
     2701 aaagcgggca tcccgtcgga gatcgggaga ccgggagttc caatgacgtg gcgctggcgg
     2761 cgacgatcag ggcggctgcg ccgcaccaga tggcgcgggg gagggaggga ccggggatcg
     2821 aacttcgtcc ctgcgacctg cgccagaacg tcagggaggg gagggagggg aacctgatcc
     2881 tcttcctcgt cgacgcgagc ggctcgatgg cagcccgcaa acgtatgtct gccgtcaagg
     2941 gcgcggtcct gtctttattg aacgatgcct accagcgccg cgacaaggta gccttgatct
     3001 ctttccgcaa tgaagacgcc aggatactcc tgcccccgac gtcgagcgtg gaactggccg
     3061 cctcgcgcct ggaggagctt ccgacagggg ggcgtacacc gctcgcagcc ggtctcgaga
     3121 aggccgccga ggtgctgaaa agggagaagc tgcgcgacag ggagcgccgt cctctgctgg
     3181 tcctgctgac cgacggacgc gcgacggcgg gacccgaccc gctgacggca gcggcacggt
     3241 tgcgttccct cgtggcgacg tcgttcgtgg ttgacacgga ggaaggttac gtcaggcttg
     3301 gaatggcggg cgagatcgca gaggctatgg aggcgcggtg cctgcgcctg gaggaattgc
     3361 gggccgattc gctcgtggat ctcatcgaga ggagaagggt ggcgtgagcg aggtggacga
     3421 agccagagaa cccagggaac ggcgccttag aaaaaggtcc aatcgagaaa gaccgctact
     3481 gatcgtcaat accggccacg gcaaaggcaa gtccacagct tccttcggaa ttatgctgcg
     3541 gggctgggca cgaggataca agataggtgt ataccagttc gtgaagagcg gcaagtggaa
     3601 ggtcggcgag caccaggccg ctctgaaact gggcaatatc gagtggttca agatgggcga
     3661 cggctggacc tggacctcga aagacctcat cgagagcgcg gacaaggccc gcgagggttg
     3721 ggaagaggtc aagcgccgcc tggccgacga gacttacgac atgctgcttc ttgacgagtt
     3781 cacctaccca atgaagttcg gctgggtgga cacgaacgag gtcgtcgagg tcctcaagaa
     3841 ccgtcccggg ttccagcacg tcatcatcac gggccgcgac gccccgcccg agctggtgga
     3901 gatctccgac ctcgtcagcg aggtgaacaa ggtgaaacac cccttcgacg agggctacag
     3961 gggccagaaa ggcatcgagt ggtagacacc ccacgcatag tagttgcggg tacccactca
     4021 ggcgtcggca agacgaccgt cgcctcgggt ctgatggccg ccctctcctc gaatggctac
     4081 agagtagcgc ccttcaaggt cggccccgac ttcatagacc cctcgtacca ttcgctcgcc
     4141 acaggccatc caggacgcaa cctcgacgcc ttcctctcgg gtccagcctt gatcggtccg
     4201 ctcttcgccc acggcgcact gggggcggac atcgccgtga tcgaaggcgt catgggcctc
     4261 ttcgacggca agagcggcgg agaactcgcc tcgacagccc acgtcgccaa actgctgaac
     4321 gcgcctgtcg tgctcgtggt cgatgcacgg gcgatgtccc gctccgtggc agccctggtg
     4381 cacggtttct cgaccttcga cctgaagttg aacattatgg gtgttgtcct gaatcgcgtc
     4441 ggctcatcaa cgcacgagaa gatgctccgc gaggccctga agcccctggg aatccccgtc
     4501 ctcggcgctc tgcgccgcga tgtcacgata cagacaccgg acagacacct cggccttgtc
     4561 cctgtcgcag agcgccgcga agaggctgga cgttcgctcg acgctctcgg aaccgtaatc
     4621 tcacgttacc tggacctcga cgacatagtg aggatcgccc gctccgccga accgctgaaa
     4681 gccgggccct ggagtcccga gttttcggag ggacagaggg tggatggcgt caggatcgcg
     4741 gtcacagccg gaccatcttt taccttcatg tacagggaga atcttgaatt gatggagagt
     4801 gcgggagccg aggtcgtcac cttcgacccg acggaagacg aagacctgcc tgacggaacg
     4861 gatgctctct atatcggtgg cgggttcccc gaggcctacg cagaaattct ctcctcgaac
     4921 ggacggatgc gcgagaaggt ccgcgcgttc gccgcgagcc gtcgtcccat cgtcgccgag
     4981 tgcggcgggc tgctctacct gttgcgcgaa ctggatggtc atccaatgtg cggcgtcctc
     5041 gacgccacgg ccaggatgac ggaccggctc acgctcggtt acagggaagc acgcgcgctc
     5101 acggtttcgc cgctggcaga gacgggaacc agcgtgcgca gccacgagtt ccactactcg
     5161 tccgtcgagc cgggagccgg tgagaccccg gcatgggagc tggccggtcg cggtccggag
     5221 ggcttcgtgt ctggtggcgt gcacgcgagt taccttcaca cccactgggc ggcgacgccg
     5281 gagctgccgc gccgcctcgt acgggccgcg tcgcggagtg cggtggaggc tacggtatga
     5341 gtgggcgtct cgtcggggtg ggggtagggc cgggagaccc tgaactgttg acgatcaaag
     5401 gtctgcgtgt gctgcgggag gctgacgagg tcttcgtgcc cgtggcggat acgggtgagg
     5461 tcgggcgggc ggaggcgacg gtgcgcgagt atctggatga aggaaggatc agacgcctcc
     5521 tcttcgctct ctcggacgat gcggaggcgc gggagcgcaa ctggacgaat gcggcgcggg
     5581 aggtctcgga gcacctgcga ggcggcaaga cgtgcgcctt cgccactatc ggtgacccta
     5641 acgtctattc gaccttcacc tatctggcac gtaccgttcg ggggttcgta cctgaggtag
     5701 aggtagagac tgtgcctggg atcacggctc tgcaggacct ggcttcgcgg agcgggaccg
     5761 tcctactcga aggcgacgag cgacttgcgc tcctaccttt cacggctggg gaggagcctt
     5821 tgagggaggc gctcgccggg ttcgagacgg tagtctgcta caagggcggg agccggctcg
     5881 gggaggtgct cggggttgcg gaggagggag gacggttgga gggcggtgtg tacggggccc
     5941 ggctcggcat cgagggtgaa gaggtcgttg ctgcgcggga gatggtcggg cgggagggaa
     6001 cctatctatc tactgtgatc tttaccgag
//
LOCUS       DATAIR010000408        13222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_395321, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000408 DATAIR010000000
VERSION     DATAIR010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395321"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VHM69_09900"
     CDS             complement(<1..1184)
                     /locus_tag="VHM69_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2740751.1"
                     /translation="MGYLPIEDHGIIGNLHTAALVGKDGTIDWMCLPAFDSPSVFCSI
                     LDDEKGGHFALRPVEYARSQQLYLPDTNVLLTRYFSPDGLAETLDFMPTITSHGERHR
                     LVRNVRVIRGRMNFLVECLPAFDYARGSHSVSVVKNSGAVFASGDHRLGLATDMRLEE
                     NAKGAAGARFTLEEGERATFVLAHLEQGEGPGEILSHVRFEELLNETLRYWRRWLSNS
                     TYHGRWREMVHRSALVLKLMVYDPTGALIASPTMGLPERIGGARNWDYRYTWLRDAAL
                     ALYALIYVGFEDEARNFMGWLRDRCQQDVDGLLQPLYGIDGRTDITEIELTHLSGYMN
                     SYPVRLGNGAHTQKQLDLYGAVLDAAYLYNKHGAPLDYDLWKSLQKILDWLSENWREP
                     DEG"
     gene            complement(1251..1673)
                     /locus_tag="VHM69_09905"
     CDS             complement(1251..1673)
                     /locus_tag="VHM69_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2740752.1"
                     /translation="MRATLILAVLAGLCVAVQTSLTGAAQRVLGPPVLVAISGLTTGI
                     VALAVSLVVAKPEYTGRAVGYAVSSGILGAVIVGSIAVAAGQAGVARALSLVIATQLI
                     AGLVLDAFGIFGAGADFSALKALGVLLIIAGGALVVSF"
     gene            1837..2553
                     /locus_tag="VHM69_09910"
     CDS             1837..2553
                     /locus_tag="VHM69_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX2740753.1"
                     /translation="MLGGARITYLGHATFRFTTAGGEQIIIDPFLTENPQTPDELKHV
                     ADLDTILITHGHFDHFSDVEPLVGQTGAMTVSNFEIFAYLQRQGIENAMPIQKGGTAQ
                     VGGIKVTATNAFHSSSIQLDDGSSIYAGEPMGYVIEFESGFKLYHAGDTSVFGDMRLI
                     GELYKPDLALLPIGNQVVMSPFEAAHAARLLGVEHVVPIHYGTFPFLPGTPEEFQQHA
                     STLAPDLKVHVMQPGDELTS"
     gene            complement(2586..3317)
                     /gene="cobS"
                     /locus_tag="VHM69_09915"
     CDS             complement(2586..3317)
                     /gene="cobS"
                     /locus_tag="VHM69_09915"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX2740754.1"
                     /translation="MKALLSFFTVLPVRGASLEEAAREVHLLPLVGLFTGLPGAALLL
                     SSHVLSPGVAATLALGAVLLSAGLHHADGVLDVGDALMVRGSAERRREVLKDTRIGIG
                     GIGALFIVYAPTVAALAALCANSPWRAAMALLAAEVSARSAMLLTLAFGKPAEEDSST
                     VPFVRSIAGSHRSLGLVLALLASLPFLILIGGLSPVASILVPLVALGALQIARTGFGG
                     IGGDVVGAAGEACRAVVLIVLSAIM"
     gene            complement(3318..3800)
                     /locus_tag="VHM69_09920"
     CDS             complement(3318..3800)
                     /locus_tag="VHM69_09920"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX2740755.1"
                     /translation="MLGGARSGKSAFAEHVASTYGGRVLYVATAMVGEDEDLKRRVRL
                     HRKRRPAVWGTLEIGDLGEVLDAAKRWDAVLLDSLTLWVSAIEEDEALARFDRFLAGA
                     EALAVPFVLVSDEVGLGVVPESKAGRRFRDVLGLVNQRAAVAAREVHLCVAGIGVRIK
                     "
     gene            complement(3815..4885)
                     /gene="cobD"
                     /locus_tag="VHM69_09925"
     CDS             complement(3815..4885)
                     /gene="cobD"
                     /locus_tag="VHM69_09925"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX2740756.1"
                     /translation="MVKGWAQHPGGRHHGAHGGLVSRTLDVPVGDLLDFSQNINPLGA
                     PVETLEAARRALYEESGSYPDLDYPDLRAALAAYLGVSADRIVPTNGGAEALFLAARA
                     AGRGGKALVLEPTFSEYAAAARAAGLETVRRVARHREAYFRLDPTSLDDLDGISVVFL
                     CNPNNPTGDVLGRREVLGVADRVREAGAALIVDEAFADFAPEVSVADKVGEGLWVARS
                     LTKFFAIPGLRLGCLVCEDAGRIQEIQPSWSVNAIAAAAGIAAVRDRGFTEASMAELA
                     RLRGGLFKALDEFAELEPFPGAANFLLVRGPEGLAGRLARRGVLVRGCGPFEGLGPEY
                     FRVAVRGEAESGRLISEIREAL"
     gene            complement(4878..5801)
                     /gene="cbiB"
                     /locus_tag="VHM69_09930"
     CDS             complement(4878..5801)
                     /gene="cbiB"
                     /locus_tag="VHM69_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX2740757.1"
                     /translation="MRHGGQVAAALLLDAMFGEPPEPVHPTVLMGRAISAFEKRALRL
                     ERARRLAGIVLALSLPSLVFVSTRMVLNVVPGRLRWMMGTALISTTLSMRGLAEAAMN
                     VERELGEGRLETARVEVGRFVGRDTRDLSEQEVCRAAVESVAENASDGIVGPMLYGLL
                     FGAPGALAYKAVNTLDSMVGYTQPPYAEIGWASARLDDFVNLGPSRATVLATAAVSRR
                     PLRTLGIANQYGPLTASPNAGRVEAAFAGALGVRLGGTNYYGGEVREGPVLGEGRLPV
                     AADIVRAVRLMRRCCALLAVLAVVVERIRGG"
     gene            complement(5798..6448)
                     /locus_tag="VHM69_09935"
     CDS             complement(5798..6448)
                     /locus_tag="VHM69_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740758.1"
                     /translation="MGPIRTGFVYGIAGVLVALGSDLYFLFVDPAETQGWVLAVIAQY
                     RTRLALAVFLFLAVLAALRVRPTRLDPGVPYRSILLRDGALAATVVAVMVGITLFLTT
                     ALQATLLAGAARDYASEAAPRIVTYVNEVRTEIRQERKERGAPQEEVRDLPPPAEVSA
                     VEATLQPPTLRDLGRSMANFVLRAVLLGTAGGLVGVLRGRPGSTDNGKPREALRRE"
     gene            complement(6526..7362)
                     /locus_tag="VHM69_09940"
     CDS             complement(6526..7362)
                     /locus_tag="VHM69_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2740759.1"
                     /translation="MLAVSAAAIFIRLADAPAVAIAFWRCALGAVVLLPPALVRRDPF
                     PKGRALYVGIASGAALGAHFGFWISSLDYTSVAASVVLVSTQPVFVAVLAYLILGERT
                     TPLSFLGIVVALAGTAVIAFDKTVGTAALYGNALALIGAVTVAVYVLIGRASRTGGVG
                     VLPYSIVVYSAAAATLLPVALILDNRLWGYSGETWFWLAAITLGPQLMGHTVFNWALR
                     YVEASIISGTVLAEPVVAALLAWLVLSEKPGLLTLVGGAVVLGGLYCLLKGRSVTVEP
                     VA"
     gene            complement(7371..7967)
                     /locus_tag="VHM69_09945"
     CDS             complement(7371..7967)
                     /locus_tag="VHM69_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX2740760.1"
                     /translation="MKTLVLSGILGELRGLLGVYRGRSPAGGGGSDGPRVAVVLGAQV
                     LRGGRPSRTLEARTRHAGELYARGEVDLLIPTGGEGEHPPAEAMVMSAILRDMGVPEA
                     AILREDTAKSTWESAVRLAEIARAKRLPEVLVVTDPLHCVRTVASFGRAGLLASAEPV
                     YSSPMWRKKWSRRGQFVRESGALVWYRIRYGVGAHSRP"
     gene            complement(7964..8554)
                     /locus_tag="VHM69_09950"
     CDS             complement(7964..8554)
                     /locus_tag="VHM69_09950"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX2740761.1"
                     /translation="MPASGIILAGGGSRRMGRDKLSIEVGGQTLIQRVHTALIGVCQE
                     VLVVGRDAIRLEGVRRVGGERPGGQGPLAGMEAGLAAARYPLAFVAAGDMPFLRESMV
                     GYLLDRLETGDALAVVPLYQSRVHPLCAAYSRSLLPRARTALDSGTRAMHEFLEGIDE
                     VAYVGEELRRFGDPALLLMNVNSPDDLERARSEAER"
     gene            complement(8563..9552)
                     /locus_tag="VHM69_09955"
     CDS             complement(8563..9552)
                     /locus_tag="VHM69_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX2740762.1"
                     /translation="MNLSGALIGFGILQAVLYLLFIRAIDLYEREPLRYVVPVFIWGF
                     AVATTVSLVFNTLFQLTLSSVTSVQTANFFTAVLVAPVVEESSKGFALLLIFFIAYLV
                     RRRSGLIEFAGVMDGIVYGSAVGFGFAIAEDILYGLQYGPETFVVRRIFGGFAHAAFT
                     SLTGIGFGLIPWVHNMMLKVSLPLLGLFGAIMLHAAFNFTATLFGPVGYLVLFFVIMA
                     YIILIIVWLAIERQIIRTELYEEVGAGTITTQEYAILPSYFLRTWYYLGLIFTGKLGA
                     WTRARRLHASAVDLALAKRLARRSRTPGSIDRVRMLRQKIKAMREGAGSMAAS"
     gene            9636..10130
                     /locus_tag="VHM69_09960"
     CDS             9636..10130
                     /locus_tag="VHM69_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2740763.1"
                     /translation="MDRYLQDRGSIKAGLRYLTHGVYVLGTRRGRQSNAMTASWVMQT
                     SERPPCVAVAVRSDRYSHDIVLESETFALSVLRDDQVDVATHFAETSGEYYDKLQGVP
                     YGLTPGGSPFLLDCLAYLDCKVIDRARAGDHTVVVGEVTASDTLDPSYPLIYDPSEYE
                     EALR"
     gene            10131..10595
                     /locus_tag="VHM69_09965"
     CDS             10131..10595
                     /locus_tag="VHM69_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2740764.1"
                     /translation="MGTDPETRLRDLGFELPDVPAPAGAYVPATRAGDLIFTAGQLPF
                     DAGELHLKGKVGDAVSLDEAQEAARLCALNALAAAAVETGGLGGISRIVKVTGFVASA
                     PGFNGQAEVLNGASDLIGEVFGDAGLHVRSAVGVAELPLDVPVEVELVVQAR"
     gene            10682..10930
                     /locus_tag="VHM69_09970"
     CDS             10682..10930
                     /locus_tag="VHM69_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX2740765.1"
                     /translation="MDATRERTAIKLYTGDYCPFCLRVKSELQRLGLDYDEVDADADW
                     REQVIQLSGQRAIPILTIGDEVLVDSSHIIRELRSRYA"
     gene            complement(10940..11767)
                     /locus_tag="VHM69_09975"
     CDS             complement(10940..11767)
                     /locus_tag="VHM69_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740766.1"
                     /translation="MFWRDGGETPESGQEAREEPAGPVRIERDAPRPATILRVAGELE
                     ARGGTILELFKEIESPLGRVVLPIYLRQEERDFFVEVETGPWDTRKSEAAVERATILR
                     SSEHAEAGLEILSGYPVPPEVEFFFGRSPAALLQLDLMRLTPDRPEASADHFREVGST
                     HWGVDLDYEPGYLPLVEELLLAALEGDEEQPVPPISDGLVAGLGCFLGETIRRNVDPI
                     GTWGPPEEWGEGPVVEVEDFVLAPVGKARAFLEQGPEESLAFYAGYVLDQLDGKSGD"
     gene            11841..12512
                     /locus_tag="VHM69_09980"
     CDS             11841..12512
                     /locus_tag="VHM69_09980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2740767.1"
                     /translation="MDYPYPMPDPQELLLVRHGQSTANARGIWQGQMEFPLSEEGRRQ
                     ASLAGRALSREPFEAIYSSPLSRAFETAQIIRAQTGFAGEVVPVSGLSERHGGILEGH
                     TWAEQERRNPDLAKKFLAIPEEERWTLVGAETDEEVIARFEEAVSSIHARHQDGARLV
                     IVSHGGVMRAFLRERFGPEVLPGAQRAANASITRLQWGDTGQRLIEVASTAHLTDDPG
                     ATTVE"
     gene            complement(12534..12683)
                     /locus_tag="VHM69_09985"
     CDS             complement(12534..12683)
                     /locus_tag="VHM69_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX2740768.1"
                     /translation="MVRPTGEDLFVHHSEVQGDPADLSPNGEVEYEVGRNDRGPNARR
                     VQPLG"
BASE COUNT         2522 a         4429 c         4101 g         2170 t
ORIGIN      
        1 ccttcgtccg gctcacgcca gttctcgctg agccagtcga ggatcttctg gaggctcttc
       61 cagaggtcgt agtcgagtgg tgctccgtgc ttgttataga ggtaggccgc gtcgaggacg
      121 gcgccgtaga ggtcgagctg cttctgggtg tgcgcaccgt tgccgagccg cacgggatag
      181 gagttcatgt atccggacag atgcgtgagt tctatctcgg ttatgtccgt acggccgtct
      241 ataccgtaga gtggttgaag gaggccgtcc acgtcctgct ggcagcggtc cctgagccaa
      301 cccatgaagt tacgcgcctc atcctcgaag ccgacgtaga tcagggcgta cagcgcgagc
      361 gcggcgtccc tgagccaggt gtagcggtag tcccagttgc gcgcgccacc gatcctctcg
      421 ggcaacccca tggtcgggga ggcgatcagg gcccccgtcg ggtcgtagac catgagcttt
      481 aggacgagcg ccgagcggtg gaccatctcc cgccaccgtc catgatatgt gctgttggag
      541 agccagcgcc gccagtaacg tagcgtttcg ttcagcaact cctcgaaccg gacgtgcgag
      601 agtatctccc caggtccttc accctgttcg aggtgcgcca ggacgaacgt cgccctctcg
      661 ccctcctcca gcgtgaaccg cgctcccgca gcccctttag cgttctcctc cagccgcatg
      721 tcggtcgcga gcccgagacg gtgatccccc gaagcgaaga ccgcccccga gttcttcacc
      781 accgagacgg agtgactccc gcgggcgtag tcgaaggccg gcaggcactc gacgaggaag
      841 ttcattcttc ccctgatgac ccttacgtta cgaaccagcc tgtgacgttc accgtgactc
      901 gttatggtcg gcatgaaatc cagtgtctca gccaggccgt caggggagaa gtaacgggtc
      961 aagagcacgt tcgtatcagg cagatagagc tgctggctgc gggcgtactc caccgggcgc
     1021 agggcgaaat gaccgccctt ctcatcgtcg aggatggagc aaaagacgct cggggagtcg
     1081 aaggctggca ggcacatcca gtcgatagtg ccgtctttgc ccacgagcgc ggccgtatgg
     1141 aggttgccga tgatcccgtg gtcttctatt ggcagatacc ccactccagc ctcctgagct
     1201 ttccacatcc ggatgcgcag cttccctgta tcttagcccc tgacggggag ttagaagctt
     1261 acgaccagcg cgcctcctgc gatgataagg agtactccga gcgccttcaa agcgctgaag
     1321 tctgcgcctg cgccgaagat accgaaggca tcgagcacga gacccgcgat gagctgtgtg
     1381 gcaattacga gtgagagtgc gcgcgccacc cctgcctgcc ctgcggcgac ggcgatcgag
     1441 ccgactatga cggccccgag gatgcccgag gacacggcgt accctaccgc acggcccgta
     1501 tattcgggtt tcgcaacgac gagcgagacc gccagcgcga cgatgccggt cgtgagcccg
     1561 gagatcgcga ccagaaccgg cggcccgagg acccgctggg ccgctcctgt gaggctcgtc
     1621 tggaccgcca cgcacaggcc ggccagcacg gcgaggataa gcgtcgctct catacgttcc
     1681 ccctgaggat cgtggtctcg acatgtggcg tcccgaccgg gacgcccgca ggattatacg
     1741 cgggcggcga ccgtttctcc ggtctttggc gtgggtatac cttagagtgt tcgtttacca
     1801 ttgttggcta tcccagggag gacattcgtg gaatcgatgc taggcggggc aaggatcacc
     1861 tacctcggac acgcgacttt ccggttcacg accgcaggtg gcgagcagat catcatagac
     1921 ccgtttctca ccgaaaaccc acagacccca gatgagctga agcacgtcgc ggatctcgac
     1981 accatcctga taacccacgg ccatttcgac cacttctcgg atgttgagcc gctggtgggg
     2041 cagaccggag cgatgaccgt ctcgaacttc gagatcttcg cctacctcca gcgccagggg
     2101 atcgagaacg ccatgccgat ccagaagggc ggcacggcac aggtcggggg tatcaaggta
     2161 acggcgacca acgccttcca ctcgtcgagc atccagctcg acgacggcag ctccatctac
     2221 gctggcgagc cgatgggata cgtcatcgag ttcgagagcg gcttcaagct ctaccacgcc
     2281 ggcgacacct ccgtcttcgg cgacatgcgt ctgatcggtg agctctacaa gcccgacctc
     2341 gccctcctgc cgatcggaaa ccaggtcgtg atgagcccgt tcgaggctgc ccacgcggcc
     2401 aggctcctcg gcgtggagca cgtcgtcccg atccactacg ggaccttccc gttcctgccc
     2461 ggcacccccg aggagttcca gcaacacgca tcgacactcg cacccgacct gaaggtgcac
     2521 gtcatgcagc caggcgacga gcttacttct tagcagcttt cggccccggc tcgagcgagc
     2581 cggggctaca ttatcgcgga gagcacgatc agcactacgg cgcgacacgc ctcccccgcg
     2641 gcgcctacga catcaccgcc gatgccaccg aacccggttc gggcaatctg caaggcgccc
     2701 agcgcaacca gaggcacgag aatggacgct accggggaaa ggccaccgat caggataagg
     2761 aacggcagcg acgcaagcag cgccagcacg aggccgaggg agcggtgaga tccagcgatc
     2821 gaccttacga aaggcacggt ggacgaatcc tcctccgccg gtttgccgaa ggcgagcgtc
     2881 aggagcatgg ccgagcgggc tgacacctca gcggcgagga gtgccatggc cgcgcgccag
     2941 ggcgaattgg cgcatagcgc cgcgagcgcg gctactgtag gcgcgtagac gataaagagc
     3001 gcgccgatac ccccgatgcc gatgcgggtg tccttgagaa cctctctgcg tctttcggca
     3061 ctgccgcgga ccatcagggc gtcaccgacg tcgaggacgc cgtcggcgtg gtgcaggccg
     3121 gcggagagaa gcaccgcccc gagcgcgagg gtggcggcga cgccaggaga caggacgtgg
     3181 gatgaaagga gcagggccgc cccaggcaac cccgtaaaga gcccgacgag aggcagaaga
     3241 tggacctccc gcgcggcttc ttccagagag gcgccgcgta cggggagcac ggtaaagaac
     3301 gagagcagcg ccttcaacta cttgatcctg accccgatcc cggcaacgca caggtggact
     3361 tcccgcgcag ccacggcagc ccgctggtta acgagcccga gcacgtccct gaaccttctc
     3421 cccgccttgc tctcaggcac aaccccgagc ccgacctcgt cactcaccag gacgaacggc
     3481 actgcgagag cctcagcacc cgcaagaaag cggtcgaacc gcgcaagcgc ttcatcctcc
     3541 tcgatggccg agacccagag tgtaagcgag tcgagcagga cagcgtccca ccgcttcgcc
     3601 gcatcaagga cttcgccgag gtccccgatc tccagcgtcc cccagacggc gggacgccgc
     3661 ttcctgtgca gcctgacgcg gcgtttcagg tcttcatctt ccccgaccat cgccgtcgcc
     3721 acgtagagga cgcgcccacc atacgtggat gcgacatgtt cggcgaaggc gctcttcccg
     3781 ctgcgggcgc cgccgaggat gagtgcgcgg acggctacag tgcctcccgg atctccgaga
     3841 tcagacgccc gctctcggcc tcacccctca ccgcgacacg gaagtactcc ggtccgaggc
     3901 cctcaaacgg cccgcaaccc cgcacgagca ccccccggcg ggccagccgt ccggcgagcc
     3961 cctcagggcc gcggacgaga aggaagttcg cagcacctgg gaatggctcc agttccgcaa
     4021 attcatctag agccttgaag agacccccgc gcagacgggc caattcagcc atcgaggctt
     4081 ctgtgaagcc tctgtcccgg acggctgcga tcccggctgc ggcggcgatg gcgtttaccg
     4141 accacgaagg ttgtatctcc tggatgcgtc cggcgtcttc gcagacgaga catccaagcc
     4201 gcaagccggg tatcgcgaag aacttggtga gagaccgtgc gacccagagt ccttcgccca
     4261 ccttatcggc gacgctgacc tcgggagcaa agtcggcgaa ggcctcgtcc acaatgaggg
     4321 ccgcgcctgc ctccctcacg cggtcggcca ctccgagaac ttcccgccgc ccgagcacgt
     4381 ctccggtagg gttgttcggg ttacacagga acactactga gataccgtcg aggtcatcca
     4441 gggacgtggg atccaacctg aagtacgcct cgcgatggcg ggccaccctg cgaacggttt
     4501 caaggcctgc tgccctggcc gcggcagcgt actccgagaa ggtaggttcg agcacgagcg
     4561 cctttccacc acgacccgct gccctggccg cgaggaacaa cgcctccgcc ccgccgttcg
     4621 tagggacgat cctatctgca gagacgccaa ggtacgccgc tagagccgcg cgcaaatctg
     4681 gatagtcgag gtcagggtaa ctgcccgact cctcgtagag tgcgcgccgg gcggcttcta
     4741 gtgtctccac cggcgcaccg agcgggttaa tgttctggct gaagtccagt agatccccga
     4801 cggggacgtc gagggtgcgc gacacgaggc cgccgtgcgc accgtggtga cggccgccag
     4861 gatgttgtgc ccagccttta accacctcga atcctctcaa cgacgaccgc gagaacggcg
     4921 agcaacgcgc agcagcggcg catgagcctc acggcgcgca cgatgtccgc cgctacaggc
     4981 agacgtccct cgccaaggac aggtccttcc cgcacttccc caccgtagta gttggtgccg
     5041 ccgagcctca cgccgagcgc gccggcgaat gccgcctcga cccttcctgc gttcgggctc
     5101 gcggtaagtg gaccatactg gttcgctatc ccgagcgtcc gaagcggccg acgggagacc
     5161 gcggccgttg ccaggaccgt ggcgcgtgag ggtcccaggt tgacgaagtc gtccagacgg
     5221 gcggaagccc atccgatctc cgcgtacgga ggctgcgtat agccgaccat cgagtcaagg
     5281 gtgttcacgg ccttgtacgc caacgcgccc ggcgcaccaa agaggaggcc gtagagcata
     5341 gggccgacga tgccgtcgct cgcgttctcc gcgaccgatt ccacagcggc ccgacagact
     5401 tcttgctccg agaggtcgcg tgtgtcccgc ccgacgaagc gacctacctc gacgcgcgcg
     5461 gtttcgaggc gtccctcacc caactcgcgc tcgacgttca tggcggcttc ggcgaggccg
     5521 cgcatcgaga gcgtcgtcga gatcagtgcc gtccccatca tccagcggag cctgccaggt
     5581 acgacgttga ggaccatccg tgtagagacg aacaccagcg agggcaggga gagagccagg
     5641 acgatgcccg cgagacgcct ggctctctcc agtcgcagcg ccctcttctc gaaagccgag
     5701 atcgcacgcc ccatcaagac ggtcgggtgc acgggttcgg gaggctcgcc aaacatggcg
     5761 tcgaggagca gtgctgccgc gacctgtccc ccatgcctca ttccctgcga agtgcctcgc
     5821 gaggtttccc gttatccgtg gagccggggc ggccgcggag caccccgacc agcccgcccg
     5881 ccgttccgag caggacggca cgcaggacga agttcgccat cgatcgcccg aggtcgcgca
     5941 gcgtgggcgg ttgcagcgtc gcctcgaccg cagaaacctc cgcgggtgga gggaggtctc
     6001 tgacctcctc ctgaggagcc ccgcgctctt tgcgctcctg acggatctcc gtgcggactt
     6061 cgttgacgta ggtgacgatc ctgggggcgg cctccgaagc gtaatctcgc gcggcgccgg
     6121 cgaggagcgt cgcctgcaag gcggtcgtca agaacagtgt gatcccgacc atgacggcga
     6181 ccaccgtggc ggccagagca ccgtcgcgca ggaggatcga acggtatggc acccctgggt
     6241 cgaggcgcgt aggacgcacc ctgagggcag cgagcacagc caggaacagg aataccgcga
     6301 gggccaggcg cgtcctgtac tgcgctatta ccgcgaggac ccaaccctgc gtctcggcag
     6361 ggtcgacgaa cagaaaatac aggtccgagc cgagcgcgac gagcacgccc gcgatcccat
     6421 agacgaaacc tgtcctgatc ggtcccaagc ctctccttta gtggcagtac gtatagccca
     6481 agaatacagc atccccagcg ggcgctgtgt gatctactac gcccgctagg cgaccggctc
     6541 taccgtgacg gaacgtcctt tgagcaaaca atacagcccg ccgagaacga cagcgccgcc
     6601 gaccagcgtc aggaggccgg gcttctcgga gagaaccagc caggcaagga gcgccgcgac
     6661 gaccggctcg gcaaggacgg tacccgagat gatcgatgcc tccacgtagc gcagggccca
     6721 gttgaagacc gtgtgtccca tcagctgggg cccgagtgtg atcgccgcca gccagaacca
     6781 ggtctctccc gagtatcccc acagacggtt gtcgagaatg agcgccacgg gcagcagcgt
     6841 cgccgccgcg gcagagtaga cgacgatgga gtaagggagt acgccgacgc caccggttcg
     6901 cgaagcgcgg cctatcagga cgtacacggc cacggtcacg gccccgatca gggcgagtgc
     6961 gttcccgtag agggcggcgg tcccgacggt cttgtcgaag gcgatgaccg ccgtgccagc
     7021 cagcgcgacg acgatgccga ggaaagacag cggagtcgtg cgttcaccga ggatgagata
     7081 ggcaaggacg gccacgaaga caggctgggt ggagacgagg acgacgctcg ctgccacgct
     7141 cgtatagtcg agcgaggaga tccagaaccc gaaatgtgcc ccgagcgcag cgccggaagc
     7201 gatcccgacg tagagtgcac gccccttcgg gaagggatcc ctcctgacga gcgccggcgg
     7261 caggagcact acagcgccga gggcgcacct ccagaaagcg atggcgaccg cgggtgcgtc
     7321 agccagcctg atgaagatgg cggccgccga tacggccagc accccgacgg ctacggccgg
     7381 gagtgcgccc ctaccccgta ccttatcctg taccagacca gagccccgct ttccctcacg
     7441 aactgacccc tgcgggacca cttcttgcgc cacatcgggc tactatacac aggctccgcg
     7501 gaggcgagaa gtccggcccg gccgaaggaa gcgacggtcc gaacgcagtg cagaggatct
     7561 gtcaccacga ggacttccgg cagtctcttt gcgcgtgcga tctccgccag cctcacggcg
     7621 gattcccagg tgctcttggc cgtgtcctca cgcaggatcg ccgcctcggg tacgcccata
     7681 tccctgagta tggcagacat caccatcgcc tcagctggcg gatgttcgcc ctctccgccc
     7741 gtcggtatta gaaggtcgac ctcgcctctg gcgtacagct ctcccgcgtg acgcgtcctg
     7801 gcctccagcg tccggctcgg cctcccgccc cgcagtacct gcgcaccgag aaccacggca
     7861 acgcgtggtc cgtcacttcc tccgccaccg gcaggagacc gcccccggta cactccgagt
     7921 agcccgcgta gctctccaag aatgccgctc aggaccagcg tcttcaacgt tccgcctcgc
     7981 tcctggcgcg ttcgaggtca tcgggcgagt tgacgttcat cagcaggagg gcgggatcac
     8041 cgaagcgtcg caactcctct ccaacgtacg caacttcatc tattccctcg aggaactcgt
     8101 gcatcgcccg cgtcccgctg tcgagagccg tccgagctcg tgggagcagc gagcgtgaat
     8161 aggcggcgca gagggggtgg accctgctct ggtacagcgg gaccacggcc agagcatcgc
     8221 ctgtttccag tcgatcaagc agatatccca ccatactctc cctcaggaac ggcatatcgc
     8281 ccgccgcgac gaaggctagc gggtaccttg ctgcggcgag accggcctcc atgcctgcga
     8341 gcggtccctg cccgccgggt cgttcgccgc cgacccgcct gaccccttcc agccggatag
     8401 cgtcgcgccc cactaccaat acttcctggc agacgccgat gagagcggtg tgaacgcgtt
     8461 ggatcagggt ctgcccgccg acctcgatgg agagcttgtc gcgtcccatc ctgcgactcc
     8521 cgccaccggc gaggattatt cccgaggccg gcacgtgcct gctcaggagg ccgccatgga
     8581 gcccgctccc tctctcatag ccttgatctt ctggcggagc atccggacgc ggtcaatgct
     8641 gccaggagtt ctggagcggc gcgcgaggcg tttcgcgagg gcgagatcca cggccgacgc
     8701 gtgcagcctg cgggcccttg tccaggcccc cagtttcccc gtgaagatca ggcccaagta
     8761 gtaccacgtc cgcaggaagt agcttgggag gatggcgtat tcctgcgtgg ttatcgtccc
     8821 agcgccgacc tcctcgtaga gttccgtgcg tatgatttgc ctctcgatgg cgagccacac
     8881 gatgatgaga atgatgtaag ccatgatcac gaagaacaac accaggtagc ctacgggccc
     8941 gaaaagagtg gccgtgaagt tgaaggccgc gtgcaacatg atagccccga agaggccgag
     9001 tagaggcaga gagaccttca acatcatatt gtggacccat gggatgaggc cgaagccgat
     9061 ccctgtgagc gaggtgaacg ccgcgtgggc gaaacccccg aagatcctgc gcacgacgaa
     9121 cgtctcagga ccgtactgca gtccgtagag tatgtcctcg gctattgcga aaccgaaacc
     9181 tacggccgaa ccgtagacga tgccgtccat cacgccggcg aactctatca gcccgctcct
     9241 ccgacgcacg aggtaggcga taaagaagat caggagcagg gcgaatccct tggagctctc
     9301 ctctaccacg ggcgccacca gaacagccgt gaagaaattc gccgtctgca cgctcgtcac
     9361 tgagctcaac gtgagctgga agagcgtgtt gaagacgagg gagaccgtcg tcgccaccgc
     9421 gaaaccccag ataaagacgg gcaccacgta gcgcagaggt tcccgctcgt ataggtctat
     9481 cgccctgata aagagcaggt agagtacagc ctgcaggatg ccgaacccga taagggcacc
     9541 actcaggttc acgaggctga ccttccggga agcgccatgg cccgcagtat acccgctcct
     9601 gctagaatac ttttgcccgt atcggaagga gaggtttgga caggtacctt caagaccgtg
     9661 gctccatcaa ggcgggcctg cgttacctga cgcacggggt ctacgtgctc gggacaagga
     9721 ggggccgcca gtccaacgcc atgaccgcgt cctgggtaat gcagacctcg gagcgccctc
     9781 cctgcgtcgc cgtcgccgtc cgcagcgacc gctactccca cgacatcgtc ctcgagagcg
     9841 agaccttcgc cctgagcgtc ctgcgcgacg atcaggtaga cgtggcaacc cacttcgctg
     9901 aaacgagcgg cgagtactac gacaagctcc agggcgtccc ttacggcctg acgccaggcg
     9961 gctcgccctt cctccttgac tgcctggcct acctggactg caaggttata gacagggccc
    10021 gcgcgggaga ccacaccgtg gtcgtcggcg aggtcacggc gagcgacacc ctggacccga
    10081 gctaccccct gatctacgac ccgagcgagt acgaagaggc tctacgctag atgggtacag
    10141 accccgagac ccgacttcgc gatctcggct tcgagctccc ggacgtgcca gcccccgcag
    10201 gcgcttacgt ccccgccacg cgcgcgggtg acctgatctt cacagcaggc cagctcccct
    10261 tcgatgccgg tgaactgcac ctaaaaggca aggtcggcga cgccgtgagc ctggacgaag
    10321 cacaagaggc cgccaggctg tgcgcgctca acgccctggc cgccgcagcg gtcgagaccg
    10381 gcggactcgg tgggatctcg cgtatcgtca aggtgaccgg cttcgtggcc tcggcgccgg
    10441 ggttcaacgg ccaggccgag gtgctcaacg gagcctctga cctcatcggg gaggtcttcg
    10501 gggatgccgg cctgcacgtc cgctcggccg ttggcgtggc tgagctacca ctggacgtgc
    10561 ctgtcgaagt cgagctcgtg gttcaggcac gctagcaggc tccgtttaaa agaacgccaa
    10621 tccggttaca atatattaca accatcatat taaagtagcg tttcagatca ggaggcgcat
    10681 tttggatgcg acgcgagaga ggacagcgat aaagctatac acgggcgact attgtccgtt
    10741 ctgcctgcgt gtaaagagcg agctgcagcg gctcggactc gactacgacg aggttgacgc
    10801 agacgccgac tggcgcgagc aggtcattca gctctcggga cagcgggcga tccccatcct
    10861 caccataggc gacgaggtcc tcgtcgattc ctcacacatt atccgcgagc tccgcagccg
    10921 ctacgcctga gaggggactt cagtccccgg atttcccgtc cagttgatcc aggacgtaac
    10981 ccgcgtagaa agccagagac tcctcgggtc cttgttcgag gaaagccctt gccttgccta
    11041 ccggggcgag gacgaagtct tcgacctcga cgacgggccc ttctccccac tcctcgggag
    11101 ggccccaggt tccaattgga tcgacattgc ggcggatggt ctcaccaagg aagcacccta
    11161 gcccggcgac gaggccgtct gagataggcg gaacgggttg ttcctcgtcg ccctccaggg
    11221 cggccaggag caactcttcg accagcggca ggtaccccgg ctcgtagtcc aggtcaacgc
    11281 cccaatgggt actcccgacc tccctgaagt ggtctgcgga cgcctcgggt cggtccggcg
    11341 tgagccgcat gaggtcgagt tgcagtagcg ccgcgggcga acgaccgaag aagaactcta
    11401 cctcaggggg aacagggtag ccgctaagta tctccagtcc cgcctcggcg tgctcggagc
    11461 tacgcagtat ggtggccctt tccaccgccg cctccgattt gcgggtgtcc cacggacctg
    11521 tctcgacctc gacgaagaag tccctttcct cctggcgcag gtaaatagga agaaccaccc
    11581 gccccaatgg agactcgatc tctttgaaga gttccagaat cgtgccccct cgggcttcca
    11641 gttcgccggc cacgcggaga atcgtggcgg gacgaggggc gtcgcgctcg atccggacag
    11701 ggccggctgg ctcttcccga gcttcctgac cagattcggg cgtctcaccg ccgtctctcc
    11761 agaacatccc gacaagcctc cccgtcagac cacgaacccg caaattctag catcggcaag
    11821 gatttgccgg aagcgccttt atggactacc cttacccgat gcccgatcca caagagttgc
    11881 tcctggtgcg tcacggacag tcaacggcga acgcgagagg catctggcag ggtcagatgg
    11941 agtttcctct ctctgaggaa ggccggcggc aggcgagcct cgccgggcgc gccctctcac
    12001 gcgaaccttt cgaagccatc tactcgagcc cgctgtcgcg cgccttcgag acagcccaga
    12061 taatccgtgc ccaaacaggc ttcgccggtg aggtcgtccc tgtaagcggc cttagcgaga
    12121 gacacggtgg tatcctcgaa ggtcacacct gggcggagca ggagcggcgg aaccccgatc
    12181 tggcgaagaa gttcctcgcg atccccgagg aggaacgctg gacgctcgtc ggggctgaga
    12241 ccgacgagga agtcatcgcg aggttcgaag aggccgtctc gtccatccac gcccgtcacc
    12301 aggatggcgc ccgcctggta atcgtctcgc acgggggggt gatgcgggcg tttctcaggg
    12361 agcgtttcgg gccagaggta ctgcccggcg cgcaacgggc ggcgaacgcc tccatcacac
    12421 gcctgcaatg gggcgatacg ggtcagcgcc tgatcgaagt tgcctcgacg gcgcatctca
    12481 ccgatgaccc gggcgccact accgtggaat gatctaagga atcctgtgga gcctcagccg
    12541 agaggttgga cgcggcgcgc attcgggccg cggtcgttgc gtccgacctc gtactcgact
    12601 tctccgttgg gtgagaggtc cgcagggtct ccctgtacct ccgagtgatg gacgaaaagg
    12661 tcctcgcccg ttggccttac caggaacccg taacccttct cggcgtcgaa ccacttgacg
    12721 cgcccccgct cgcgctccgg cccttcgcca ggagtcggtt cctcagaacg tccgtcgagg
    12781 gtagccgttg atggcgtcga gtcctctgac tcttcagtga aacgggattc tcccccgtcg
    12841 gcttcgagca cgatcgagtc ttcggcgacc ttcacaacca ccgactcgac ccccatccag
    12901 tgctccttcc agatcgggtc ctcggcgacg gaggggtcga gccacaggcc ccatccgtcg
    12961 cgttcgccgt gatccagaac cccctcgaag acgctctcgg gctcgcgcgg accgcgctcg
    13021 cgcctgtaga tgtcgttgcg ggatcggaaa gactgtacgg aggaggcgct ggtgccgagg
    13081 gcgtcggcga tccaatcgtc cgacttgccc tcctccaccc actccctgat ccggtccatg
    13141 tggggcttca acgctatgcg ctttctggag tctgccatgc taccgcctgc tccttctttg
    13201 aacccgttct gatagatatt gc
//
LOCUS       DATAIR010000409         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_119124, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000409 DATAIR010000000
VERSION     DATAIR010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119124"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..3396
                     /locus_tag="VHM69_09990"
     CDS             226..3396
                     /locus_tag="VHM69_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2740769.1"
                     /translation="MVESRHRASGRFYANTTLVPDGSLRVTLLGRFEVSAGTRVIRED
                     EWRLRKAASVVKLLALAPDHGLHREQVMHLLWPDLGARAAANNLHQALHVARRTIEPE
                     ASTHRFLPMRGEQLTLCQEGSAWVDVNAFERTVREARGSGRPTAYRAAIDLYSGDLLP
                     LDRYEEWAERRREELRRTYLAMLFELASVLEERGALDRAVEMLQEIVAHEPVHEEAHV
                     GLMRAYAKAGQRHRALRQYEHLRQALRSELGTEPHAASRYVYGEVLAGHVPVPSAIGR
                     EQPREIVRHNLSGSLTSFVGREREREEVERLLGTTRLLTLTGAGGSGKTRLAQEVARD
                     LTDAYPDGVWFVELAPLTHGSLVKGAVSETLDVREQPGRPPDDFLVEALREKCLLLVL
                     DNCEHLVDEVARLAEMLLSSCQGLRILATSREILGSPGEVSWQVPPLSGPDPAHRDTA
                     ETLEGYESVRLFVQRARYRNPAFILTPQNAAGVAEICGRLEGLPLAIELAAARVGFSV
                     QEIAARLDDSLRLLTAGSRTASPRQRTLRGALDWSHDLLSEPERVLFRRLSAFAGGWT
                     LEAAEVVGCGDGIERGDVLDLLSGLVDKSLVLAEATGDGAVRYRLLEPVRQYAGELLR
                     ESGEAETVRHNHARWCLDFVEGAREGLEGPEQASWVERLKKEHANAQAALSWSLDDEP
                     ETSLRLVATLGYFWYRYGRILEGRRWLEEVLERTGDVETVTRAKTLRLAGVLSEESGL
                     YEQAEKLHEQGLALYRRLENRDGVAAALTSLGALAYAVGDLERAVSLTQESLALKREL
                     GDEKALMSSRNNLGEMMQSAGDLAKAQALFEENLESDEILGDEWGAAVSRLNLGTLAV
                     EQGEPVRAEKLLTEALRAFQRLGDEDATTDCLDSLAAAAGARGEGLRAATLFGTAEAT
                     REELGTPIRPVDRERYERFVAAARSGVGERAWASAWSAGRAMSLQEAAAYACAPESTP
                     PPATDEIATAGAMSERPSTSLTPREREVALLVARGFTNRRISEELSISERTVATHVAR
                     SLKKLELGSRSGLAAWITEQSPPDPD"
     gene            3632..>4502
                     /locus_tag="VHM69_09995"
     CDS             3632..>4502
                     /locus_tag="VHM69_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740770.1"
                     /translation="METDRLRTGEQEKNLFLVTLFRNDWEMITPYDGSVNDRGDQSMK
                     RVVSLFVAIALAAGLLALPLGSAEAAFPGTNGKIIFDSDRLTAVGGPGLYTITPGGTA
                     AKIPGTSSGDNQPAWSPDGSRIAFQSGSTTNQEISVMNVDGSGRRQLTTTPVAEQEPA
                     WSPDGSQLAFVAGTSGTDATTDLEIWVMNADGSGLTQLTNTAQGVRDTEPAWSPLGNQ
                     IAFVSEGRTGDANSNIYVMDTDPATNDASNLTPDDFTTNPVYQWNDEDPSWSPDGTQI
                     TYSTVQDVWKMNAN"
BASE COUNT          861 a         1409 c         1501 g          731 t
ORIGIN      
        1 cctgctcacc cccttctcca gtgccgtcac catgctctct cggctgacgg gcttgcaccc
       61 gctggagatc ggaccgagga gcaaccttgg gttcggcctc atagtggccg tcgccgcgtt
      121 ggtctacctg actctcctga ccttgctgct cttgtaaagt gagatctaac ccgcgacgct
      181 ataaaaccca tcttgctatc atggccctgg agaggtgtct tgacgatggt ggagtctcgc
      241 catagagcat cgggaaggtt ctacgcgaat acaactctag taccggacgg aagcctgcgc
      301 gtaacactgc tcggacgttt cgaggtctca gcaggtactc gggttatccg cgaggatgaa
      361 tggcggctca ggaaggccgc gagcgtggtg aagctgctcg cgctggcccc ggatcacggc
      421 ctccaccggg agcaggtcat gcacctgctc tggcccgacc tcggggcgag ggccgcggcc
      481 aacaacctcc accaggccct gcacgtggcg agaaggacca tcgaacctga ggcctccaca
      541 caccgtttcc tccccatgcg cggggagcag ctcacgctgt gccaggaggg ttccgcctgg
      601 gtagacgtaa acgctttcga acgaaccgta cgggaggcgc gtggctccgg gcggcccact
      661 gcttaccgtg ccgcgatcga tctgtattcg ggggacctcc tgccgcttga ccgctacgag
      721 gagtgggcgg aaaggcgcag agaagagcta cgaaggacgt acctggccat gctcttcgaa
      781 cttgcgtcgg tcctggagga gcggggcgcc ctcgaccggg cggtcgagat gctccaggaa
      841 atcgtcgcgc acgaaccggt gcatgaagag gcgcacgtag gactcatgcg ggcgtacgca
      901 aaggccggcc agcgccaccg cgccctgcgc cagtacgagc atctgcggca ggcgctccga
      961 agcgagctcg gcaccgagcc ccacgcggcc agcaggtacg tttatggcga ggtcctggcc
     1021 ggtcacgtac ccgtcccatc agccatcggc cgcgagcagc cgagggagat cgtcagacac
     1081 aatctttctg gctcactgac cagcttcgtc gggagggagc gcgaaaggga ggaggtcgaa
     1141 cggttgctcg gcacgacccg gttacttacc ctcacgggag caggcggctc aggcaagacc
     1201 aggctcgcgc aagaagtagc cagggacctg acggacgcgt acccggacgg ggtctggttc
     1261 gtggagctgg cgccgctcac acatggttct ctggtgaaag gggcggtgtc tgagaccctc
     1321 gatgtgcgcg agcagccggg ccgtccaccc gacgactttc tcgtggaagc cctgcgcgag
     1381 aagtgcctcc tgctcgttct ggacaactgc gagcacctcg tggacgaagt cgcgcgcctc
     1441 gcggagatgc ttctcagttc gtgccaaggg ctcaggattc tggctacaag ccgcgagatc
     1501 cttggctccc caggagaggt gtcctggcag gtgccgcccc tttctggacc ggacccggct
     1561 cacagggaca cggccgagac gctcgaaggg tacgagtccg tacgtctgtt cgtccagcgg
     1621 gcacgctacc gtaaccccgc cttcatcctg accccgcaga acgcggcggg cgtggcggag
     1681 atctgcggtc gcctggaagg acttcccctt gccatagagc ttgcggcggc gagggttggg
     1741 ttctccgtgc aggagatcgc cgcgaggctg gacgattccc tgcgcctgtt gacggcgggt
     1801 agccgcaccg cctcgccgag gcagaggacg ctgcgagggg cgcttgactg gagccatgac
     1861 cttctctccg agcccgagcg cgtgcttttc cgtcgcctgt ctgcgttcgc cggaggctgg
     1921 acgctcgagg cggctgaggt cgtcgggtgc ggagacggca tcgagcgagg agacgtcctc
     1981 gacctgctct cagggctagt ggacaaatcc ctggtgctgg ccgaggcgac cggggacggc
     2041 gccgtgcgct accggctcct cgagccagta cgccagtacg ccggagagtt gcttcgagaa
     2101 agcggcgaag ctgagaccgt acgccacaac cacgctcgct ggtgccttga cttcgtggag
     2161 ggagcgcgag agggtctgga agggcccgag caggcatcgt gggtggagcg gctgaagaag
     2221 gagcacgcca acgcgcaggc ggcgctctcc tggtcgctcg atgacgaacc cgagacgtcg
     2281 cttcgactgg tcgctactct gggctacttc tggtaccgct acggacgcat cctcgagggg
     2341 cgtcgctggt tggaagaggt gctcgaacgc accggcgacg tcgagaccgt cacgcgggcc
     2401 aagacgctac gcctcgccgg cgtgctctca gaagagagcg gactctacga acaggcagag
     2461 aagctacacg agcaaggact ggcgctctat cggcgactgg agaacagaga cggcgtcgcc
     2521 gccgcgctca ccagcctcgg ggccctggca tacgccgtcg gcgacctgga gcgcgcggta
     2581 tcgctgactc aggagtcgct cgcgctcaag cgcgagctgg gcgacgagaa ggccctcatg
     2641 tcttccagga acaaccttgg tgagatgatg cagtcggctg gcgacctcgc gaaagcccag
     2701 gcgctgttcg aggagaacct ggagagtgac gaaatcctgg gcgacgaatg gggcgcggcg
     2761 gtcagccgcc tgaacctggg caccctggcc gtcgaacaag gcgagcccgt gcgtgctgag
     2821 aagctactga cggaggctct acgcgccttc caacgccttg gcgatgagga cgcaacaacg
     2881 gactgtctcg actcccttgc agcggcggca ggagcgcggg gtgaagggct cagggcggct
     2941 accctattcg ggaccgccga ggcgacgcga gaggagctgg ggactcccat ccgtcccgtg
     3001 gaccgggaga ggtacgagcg cttcgtagcc gccgcgcggt ccggcgtcgg cgagagagcc
     3061 tgggcttcag cgtggtctgc agggcgtgcc atgtcgctac aggaggccgc cgcgtacgcg
     3121 tgcgctcctg agagcacgcc tccgcccgcc acagatgaaa tcgcgacggc aggagccatg
     3181 tcagaacggc cttccacgtc cctcaccccg cgtgagcggg aggtcgcgct actcgtcgcg
     3241 cgggggttta ccaaccgccg catctccgag gagctctcca tctccgagcg caccgtcgcg
     3301 acccacgtcg caaggagcct caagaagctg gagctagggt ccaggtccgg gctggcggcc
     3361 tggatcaccg agcaatctcc cccggatccg gactgaaaac cagcaactat cgacgatcag
     3421 ccgccgcatc gaagtccaga tttcaagctg gagatacctc tttgcgaaaa tacgctattc
     3481 tgcttacgca tagaacactt caattaagcg ccggctaaga agcgccgttt acgctgatga
     3541 ttgagggctg tgccgggtgg tccggttgcc gaatcagaag caatgctgtg cgacgcgagg
     3601 cggcttgccg cgggagataa aggagagatg agtggagaca gatcggctgc ggaccgggga
     3661 gcaggagaag aacctcttcc tcgtaacgct tttccgtaac gattgggaga tgatcacccc
     3721 gtacgatgga tcagtcaacg acagaggaga ccagagtatg aagagagtag tttcattgtt
     3781 cgtggcgata gcgttggcgg cgggtctatt agcgctgccc ctggggtcgg cggaggcggc
     3841 gtttccgggg accaacggca agattatctt cgacagcgac cggctgaccg cagttggcgg
     3901 acccggcctc tacaccataa cccccggcgg gaccgcggcc aagatacccg gcacgtcctc
     3961 gggcgacaac cagcccgcct ggtcgcccga cgggtcgcgg atagcttttc agagcggctc
     4021 gaccaccaat caggagatct ccgtcatgaa cgtcgacggc agcggacgga ggcagctcac
     4081 aactaccccg gtggcggagc aggagcccgc ctggtcaccc gacggaagcc agctcgcctt
     4141 tgtcgcgggc acctctggca ctgatgcgac cacggacctg gagatctggg tgatgaacgc
     4201 cgacggcagc gggctgacgc aacttaccaa caccgcccag ggcgtaaggg acacggagcc
     4261 cgcatggtcc ccgctcggaa accagatcgc cttcgtcagc gaagggcgaa ccggcgatgc
     4321 caacagcaac atctacgtga tggacacgga ccctgcgacg aatgatgcga gcaacctgac
     4381 acctgacgat ttcaccacga acccggtcta ccagtggaac gatgaagacc cgtcttggtc
     4441 gcctgacggt acgcagatta cctactcgac ggttcaggac gtctggaaga tgaacgccaa
     4501 cg
//
LOCUS       DATAIR010000410         6876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_26060, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000410 DATAIR010000000
VERSION     DATAIR010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26060"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VHM69_10000"
     CDS             <1..758
                     /locus_tag="VHM69_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2740771.1"
                     /translation="GTERITVGTGIAGIYLRHSHTMATGASLIEELHPGRFLLGIGVS
                     HIPFHEEMGISPGKPLADMSQYVESIRSVTDGMTCPPIIVAALRKKMTALAGQIADGV
                     MWANAALSHVPFSLAQMTPERRAAMIISNSAPACVSEDRRAALEAIRRYLLFYVKLPN
                     YQNYFVEAGYEGEMATIRSAMEDGNDERIMAAIPERMVADVALFGAPSEVRERAEAWA
                     EAGITHLVLDTVSAVGDSLDAPYEVMAAFDRGS"
     gene            884..1408
                     /locus_tag="VHM69_10005"
     CDS             884..1408
                     /locus_tag="VHM69_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740772.1"
                     /translation="MAHRDELREHEQVLLRLLDEIGRNLARQERLLEEIENRHVHIRN
                     SWIQEGADEPFGPPDQQALVNELQRELELGRDQELELHEILDVLKRRCELEDRLLDEL
                     ESHLPREHRDHLLQDLLGRFERRDRLLEDIELKSFQEKRDRLAQTMRLLPELERLLFE
                     VVERHLVRLHNEGD"
     gene            complement(1405..1902)
                     /locus_tag="VHM69_10010"
     CDS             complement(1405..1902)
                     /locus_tag="VHM69_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2740773.1"
                     /translation="MQLYLVDGARLRELREQAGLSQEELSLTIGQSGTWAEEMEREGR
                     VHVLEIIVQKLTMIFGVEISDLSTFPDPPVPTVLEEPVLDVEKLRHLREEAALSQEQL
                     DELAELEPGSIARAESVDPTSEEIHASVDTIWRLAMALKVSPTILTRMRGDLHQIDPR
                     RPGDS"
     gene            complement(1941..2189)
                     /locus_tag="VHM69_10015"
     CDS             complement(1941..2189)
                     /locus_tag="VHM69_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2740774.1"
                     /translation="MKVDGAKLAAARERVFMSQEELAESIGMNPAVVIRLETEDRTEI
                     RENLGEDLMQILFVGRSELTSYPEPPEPPLEDPGATED"
     gene            complement(2443..3657)
                     /locus_tag="VHM69_10020"
     CDS             complement(2443..3657)
                     /locus_tag="VHM69_10020"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX2740775.1"
                     /translation="MVDWFRRRTGKDEPSNNAGEPPALDVKTETAEDLVDRTPPVPEP
                     ADGFSEAGIREALRDVRDPEIGRDLISLNMIRSIHIDGSHVTVGVALTTAGCPMKHRI
                     ITDIRDRLTMIEGVEDVEVDFGVMTDEDRQNLLTSLHGGPSELAPAFRDESKTRIIAV
                     VSGKGGVGKSTVAVNLAAALDRAGHSVEILDADVHGSSIPVMLGSLEKPNVVGGVIFP
                     IESRTGLKFISMGNFVNEGQAIIWRAPIVNKALTQLMRDVYWDEPEFIIVDMPPGTGD
                     VALTVAQMIPKAEALVVTTPQSDAARVAVKAGKMAVQAHLKVAGVVENMGHAVCPCCG
                     EQMRIFGGDGGERVAGELGAQVMGRIPIVPDATGEPGRSLFEVDTAPARAFDEIAASL
                     AATKVRRRIKVL"
     gene            complement(3841..5520)
                     /locus_tag="VHM69_10025"
     CDS             complement(3841..5520)
                     /locus_tag="VHM69_10025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX2740776.1"
                     /translation="MSFPQRLSLMAGTLMAVLFLFILVQSNMQVAKAQASPEDPTYEE
                     GTKQQFAPGEIIVTLDQSASQSDLTQLNQQNDASTEENLPQSKVNVVDLPPDLTVSEG
                     IQAYEDSPDVAYAEPNFKLFPTATPNDPYFSRMWALNNTGQTGGTIDADIDAPEQWNT
                     NTGSPLTVVAVIDEGMDINHPDLKANIYTNAVEAIGVKGVDDDRNGYVDDVHGYDFAN
                     NDASVYDPDPITGKGDEHGTHVAGTIAAVGNNGTGVTGVNWQAKVMPLKFLGPNGGYT
                     SDAVEALDYAVKNGVKISNNSWGGGGKSQALQDAIARADSAGHLFVAAAGNGGADGIG
                     DNNDVTPNYPANYPNSNIISVAATDNRDRLASFSNFGVSTVDLAAPGVSILSTLPGNT
                     YGNYSGTSMATPHVAGVAALLKSQNPILDDGGMKAQILQFSEKKTSLKNKVATGGRLN
                     AYATLTQQIAPDTTDPSIANVRPVPGSKSRDRTPTTTATVQDNRSELMKSDIRLFVDG
                     KEKTSFSYDQVGDKLSYTSARLSYSTHTIKIVADDDAGNTAIQSWRFTVRR"
     regulatory      complement(5537..5616)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5709..>6876)
                     /locus_tag="VHM69_10030"
     CDS             complement(5709..>6876)
                     /locus_tag="VHM69_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2740777.1"
                     /translation="ASILLLLYSLVAEEQGVEAHNVTGTTQNDILKEYLARGTYIYPP
                     GPSMRITTDMFAYCARELPRWNTISISGYHIREAGSTAVQELAFTLSNAIAYVEAAVA
                     VGLDVDQFAPRLSFFFNAHNDLFQEVAKYRAARRMWARIMRERFGASDERSLKLRFHT
                     QTAGSSLTAQQAENNVVRTTVQALSAVLGGTQSLHTNAFDEALALPTERSARIALRTQ
                     QILASEGGLTGTADPLAGSYYVESLTDAVEEEAWTYIQRIDEMGGSVKAVEERFMQRE
                     IEDAAYRYQREVEKGERVIVGVNRYTSGGLEDPDMELHSVDEGIRERQTERLAELKDT
                     RDNAAVQKALADLKGAAEGTRNLLYPMKEALSGLATLGEVSDTLRGVFGEYRPV"
BASE COUNT         1234 a         2101 c         2070 g         1471 t
ORIGIN      
        1 acgggacgga gcggattacg gtcgggaccg gcatagccgg catctacctg cgccattccc
       61 acacgatggc gaccggggcg tcattgatcg aggagctcca ccctggtcgc tttctgctgg
      121 gtatcggcgt ctcgcacata ccgttccacg aggagatggg catcagcccc ggaaagcccc
      181 tcgccgacat gagccagtac gtcgagagca tacgttcggt gacagatggg atgacgtgtc
      241 cgccgataat agtcgcagca cttcgcaaga agatgacggc ccttgccggg cagatcgctg
      301 acggggtaat gtgggcgaac gcggcactct cccacgtgcc attctcgctc gcccagatga
      361 ctcccgagcg ccgcgcggcg atgataataa gtaactcggc tcccgcctgt gtttcggagg
      421 atcgcagggc ggcgctcgag gcgatacgaa ggtacctgct cttctacgtg aagctgccca
      481 attaccagaa ttacttcgta gaggccggct acgaagggga gatggcgacc atccggtccg
      541 ccatggaaga cggcaacgac gagagaatca tggcggcgat cccggagaga atggtggccg
      601 acgtcgccct cttcggagca ccctccgagg tgcgcgaacg agccgaagcc tgggccgagg
      661 caggcataac ccacctcgtc ctggatacag tctccgcggt cggtgactcg ttagacgccc
      721 catacgaagt catggcggcc ttcgatcgag gctcctgaca attctcccgg cttgcgccat
      781 gcgacgcacc ccgcttggtc tctgatcttc attcttgcct gacttacctt caggtacgtg
      841 cttgtttaca atcatggccg ggagcgagga atggaggagg cccgtggcgc acagggatga
      901 actccgagag cacgagcagg tcctgctccg attgctggac gaaataggga gaaatctcgc
      961 ccggcaggaa cggttattag aagagataga gaaccggcac gtccacatac gaaactcctg
     1021 gatacaggag ggggcggacg agccgttcgg gccgccggat cagcaagcgc tcgtaaacga
     1081 gttgcagcgt gaactggagc ttgggcgtga ccaggagctc gaactacatg agatcctgga
     1141 cgtgttgaag cgccggtgcg agctggaaga ccggctactc gatgaactgg agtcccacct
     1201 gcctcgggaa catcgggacc acttactaca ggacctgctt ggccgtttcg agcggcgcga
     1261 ccggctcctt gaggacatag agctcaaatc tttccaggag aagagagatc ggctggcgca
     1321 gactatgcgg ctcctgccgg aactggagag gctactcttc gaggtcgtgg aacgccatct
     1381 cgttcggctc cacaacgagg gtgattaaga gtctccaggg cggcgagggt cgatctgatg
     1441 gagatcgccc cgcatcctgg tgaggatcgt gggcgagact ttcagggcca tggccagcct
     1501 ccagatcgtg tccaccgagg cgtgaatctc ctcggaggtc gggtctacgc tctccgccct
     1561 cgctatcgag ccgggctcca gctccgcaag ctcgtcgagt tgctcttggg agagggcggc
     1621 ctcctccctc aagtggcgta gcttctccac gtccaggacc ggctcttcga gaacagtcgg
     1681 aacaggcggg tcggggaagg tagacaggtc agagatctcg accccgaaga tcatggtgag
     1741 cttctggacg atgatctcga ggacgtgtac ccgaccctcc ctctccatct cctcggccca
     1801 tgtgccactc tggccgatcg tcaaggacaa ctcctcttgc gagagaccgg cctgctctct
     1861 tagttcccgt aaacgagccc cgtctaccag gtacagttgc atctccgtcg ttcctttccg
     1921 gctcgttgag gtctcgctgc tcaatcctct gtggcgcccg ggtcttcgag gggcggctcc
     1981 ggtggttcag ggtagcttgt caactccgac ctccccacga agaggatctg catgagatcc
     2041 tcgccgaggt tctccctgat ctcggtccgg tcttcggtct ctagtcttat taccaccgcg
     2101 gggttcatgc cgatggattc ggccaactct tcctgcgaca tgaacactct ctccctcgca
     2161 gccgccaact tcgccccgtc taccttcacg accacacctc ctcgctctgt cttcagcgtc
     2221 tggtccacga tagccgtgac cggaagcgat cgccaaatac cgcgctcctg gcagacattg
     2281 tccccacatc tttgtggtgc aattctaccc aaaggccgac ccgaagtcgt cgtgagtccc
     2341 cggctcgacc cgatcatcac cctggtggcc gcacaagtga gcgcgccaga cgcctgatga
     2401 ggaagcggcc ccgcacgggg ccactccatc aaatcctgat gcctacaaca cctttatcct
     2461 gcgtcggacc ttggtcgctg caaggctcgc tgcgatctcg tcgaaggctc gcgctggagc
     2521 tgtgtccacc tcaaagagcg atctccccgg ctctcccgtc gcatctggaa cgatcgggat
     2581 gcgtcccatt acctgcgctc ctagctctcc cgccaccctc tcgcctccat ctcctccgaa
     2641 gattcgcatc tgttctccgc agcatgggca cacagcgtga cccatgttct caaccactcc
     2701 tgctaccttc aggtgtgcct gcactgccat cttgcctgcc ttaacagcta ctcgtgccgc
     2761 gtctgactgc ggagtcgtaa caacaagagc ctctgccttg gggatcatct gagctaccgt
     2821 taaggctaca tctcccgtcc ctggtggcat gtcaacgatg atgaactctg gctcgtccca
     2881 gtagacatcc ctcataagct gcgttagtgc cttgttcact atcggtgcac gccatatgat
     2941 cgcctgtccc tcgttgacga agttgcccat ggagatgaac ttcaacccag tcctcgactc
     3001 tattgggaag atcactccac caacgacgtt tggcttctct aaagagccta gcatcacggg
     3061 aatcgatgag ccgtgcacat ccgcatcgag tatctcgacg gagtgacccg ccctatccag
     3121 agccgctgcg aggtttacag ctaccgtgct ctttcccact cctcccttcc cagagaccac
     3181 ggcgatgatg cgtgtctttg actcgtcgcg gaaagcggga gctagctccg acgggccgcc
     3241 gtgcagcgaa gtgaggaggt tctggcgatc ttcgtcggtc atcacgccga agtcaacttc
     3301 gacgtcctcg acgccctcga tcatcgtgag cctgtctcgg atatcggtga tgatcctgtg
     3361 cttcatgggg catccagcgg ttgtcagggc tacgccaaca gttacatgcg atccatcgat
     3421 atggattgag cggatcatgt taagcgagat gaggtcgcgt ccgatctcag gatcgcgtac
     3481 gtccctcaac gcctcccgaa tgccggcttc cgagaagccg tcagcgggct caggcacggg
     3541 gggtgtgcga tccacaaggt cttcggccgt ctcggtcttc acgtcgagcg cagggggctc
     3601 ccccgcattg ttcgatggtt cgtccttgcc ggtgcgtctg cgaaaccaat caaccacgtt
     3661 tgccaacttc ctccggtgcg agcgaccggc gattcgaacg ggctcgaaag agcggtagat
     3721 gcagcattct tcagcccaat attaacataa ggtccagttg cctccgcgtg ggttcagcgc
     3781 gtgcgaaaag agaccggggc agtcgccccg gtctctgact tcgccttccg gacgatcctt
     3841 ctaccgcctg accgtgaacc gccaactctg gatcgcggta ttgccagcgt cgtcgtcggc
     3901 tacgatcttg atggtgtgcg tggagtagga gagccttgcg ctcgtgtaac tgagcttatc
     3961 cccgacctga tcgtaggaga aactcgtctt ctccttccca tcgacgaaga ggcgtatatc
     4021 ggatttcatc agctctgacc ggttgtcctg aaccgtagcc gtggtggtcg gcgtacggtc
     4081 gcgggacttc gagccaggta ccggcctcac gttcgcaata ctcggatccg tagtatccgg
     4141 cgcaatctgc tgggttaggg tggcgtaggc gttgagcctg ccgccggtcg ccaccttgtt
     4201 cttaagcgag gtcttcttct ccgaaaactg gaggatctgc gccttcatcc cgccgtcgtc
     4261 caggatcggg ttctggctct tgagcagcgc cgccacgcca gcgacgtgcg gcgtggccat
     4321 cgaagtgccg ctgtaattgc cgtaggtatt ccctggcagc gtgctcagga tgctgacacc
     4381 gggagcggcg agatcgaccg tactaacacc gaagttcgag aaggaagcca gcctgtcgcg
     4441 gttgtcggtc gcggcgacgg agatgatgtt ggagttcggg tagtttgcag gatagttcgg
     4501 ggtgacgtcg ttgttgtcgc cgatcccgtc cgccccgccg ttgccggcag cggcaacgaa
     4561 gaggtgtccc gcagagtccg cgcgggctat ggcgtcctgc aaagcctgcg acttcccgcc
     4621 gccgccccag gagttattgg agatcttcac gccgtttttc acggcatagt cgagggcctc
     4681 gaccgcgtcc gaagtgtagc cgccgttcgg gccgaggaat ttgaggggca tgaccttcgc
     4741 ctgccagttg acgccggtca cgccggtgcc gttgttgccg acggcggcta ttgtgcctgc
     4801 gacgtgtgtg ccatgctcgt cgcccttgcc agtgatcggg tccggatcgt agacggaggc
     4861 atcgttgttg gcgaagtcgt agccgtggac gtcgtccacg tagccgttcc tgtcgtcatc
     4921 gacgcccttg acgccgatgg cctcgacagc attggtgtag atgttagctt tcaagtcagg
     4981 gtggtttatg tccatgcctt cgtcgatgac ggcgacgacg gtcaatgggc tacccgtgtt
     5041 ggtgttccac tgctcgggcg cgtctatatc ggcgtctatc gtgccgccgg tctgtcctgt
     5101 gttgttcaga gcccacatcc tggagaaata agggtcgttc ggggtcgccg tcgggaacag
     5161 cttgaagttg ggctcggcgt acgcgacgtc cggagagtcc tcgtaagcct gtatgccctc
     5221 ggacacggta aggtctggcg gcagatccac gacgtttacc ttgctctgag ggaggttctc
     5281 ctcggtgctc gcgtcgtttt gctggtttag ctgcgtgagg tccgactgcg aggcggactg
     5341 gtcgagcgtg acgatgatct cgccaggggc gaactgttgc ttcgtccctt cctcatacgt
     5401 agggtcttcg ggactggcct gcgccttcgc aacctgcatg ttgctctgaa ccagaatgaa
     5461 caggaacagg acggccatca gggtaccagc catgaggctg agcctttgcg gaaacgacat
     5521 gctgatggga tctcctttcg gtaacccggc tgcctctttc acaagaggtc cgtggctttg
     5581 cgtccccgcc tcacgacggg ttagccttta tcgtggattc ttgaattata cccgaaatct
     5641 atattcgaaa aaccgtagag gtttggtcac cacagtcaca tttgcaggtc tttcggacct
     5701 ttccgccctc agacgggacg gtattctccg aagaccccgc gcagcgtgtc ggagacctcg
     5761 cccagcgttg cgagtcctga cagggcctcc ttcatcgggt agagaaggtt ccgggtacct
     5821 tcggcggcgc ccttcaggtc ggcgagcgcc ttctggacgg cggcattgtc acgggtgtct
     5881 ttgagttcgg cgaggcgttc cgtctgacgt tcgcggatac cttcgtcgac gctgtgaagc
     5941 tccatgtctg ggtcctccag tccgcctgag gtatagcggt tcacgcctac tatgacgcgt
     6001 tcacccttct cgacctcacg ctggtagcgg taggcggcgt cctcgatctc acgctgcata
     6061 aagcgctcct cgacggcctt taccgagcct cccatctcgt cgatgcgctg aatatacgtc
     6121 cacgcctcct cctcgacggc atcggtgagc gactcgacgt aataggagcc ggcgagcggg
     6181 tcggccgtcc ccgtcagccc gccttcgctg gcaaggatct gctgggtcct cagggcgatg
     6241 cgggcgctcc gctcggtcgg gagggctagc gcctcgtcga aggcgttggt gtgcagactt
     6301 tgtgtgcccc caagcaccgc cgaaagggcc tgcacggtgg tgcggacgac gttgttctcg
     6361 gcctgctggg ccgtaagaga agagcctgct gtctgggtgt ggaagcggag cttcaggctg
     6421 cgttcgtcgg aggcgccgaa gcgctcgcgc atgatcctgg cccacatcct cctcgccgcg
     6481 cgatacttgg ccacctcctg aaagaggtcg ttgtgcgcgt tgaagaagaa cgagagcctg
     6541 ggggcgaact ggtcaacatc gagcccgacc gcaacggcag cctccacgta ggctatcgcg
     6601 ttcgatagag tgaacgcgag ctcctggacg gcagtggatc cagcctcacg gatatgatat
     6661 ccgctgatgg agatggtgtt ccagcgcggc aactccctgg cgcagtaggc gaacatgtcg
     6721 gtcgtgatcc tcatcgaagg tcctggcggg tagatgtagg tgccgcgcgc caggtactcc
     6781 ttgagtatgt cgttctgggt ggtaccggtg acgttgtgcg cctccacgcc ctgctcctcg
     6841 gcgacgagcg agtagaggag aaggaggatc gaagcc
//
LOCUS       DATAIR010000411         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_196588, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000411 DATAIR010000000
VERSION     DATAIR010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196588"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VHM69_10035"
     CDS             <1..496
                     /locus_tag="VHM69_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEX2740778.1"
                     /translation="FVRLARRTPRLKSVPPRDVPASVLPNLRRFVWIHRADTTRQAVS
                     LWKALQTQQWRRDSDEELGGRGLRFSFAAVDHLKLRIDEHNDAWQNFFEECGVEPMEV
                     VYEELVEDYEGTVLWLLDGIGVSIPENFGVTEPKMRRQADDLSEEWVRLYNERAAVKS
                     AQKG"
     gene            500..1291
                     /locus_tag="VHM69_10040"
     CDS             500..1291
                     /locus_tag="VHM69_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2740779.1"
                     /translation="MPDLPNILYIHSHDTGRWVRPYGYSVRTPNIQRLAEEGVLFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLKDYRHHIVHTLRDLGYWSALIGE
                     QHISKKPDVIGYDEVIKISTSHTDDVVPVTLDLLERASDRPFFLSVGFFETHREWFRP
                     SSPKKANYVIPPPNLPDAPETRLDMAAFQESARSLDRGIGAVLDALDAEGLADDTLVI
                     CTTDHGLAFPGAKATLYDRASASCSSCAGQAVSPVAGRATPSSPT"
     gene            1222..1800
                     /locus_tag="VHM69_10045"
     CDS             1222..1800
                     /locus_tag="VHM69_10045"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEX2740780.1"
                     /translation="MLILRGPGGFTGGRASDALVSHIDLFPTVCDLIGVERPEWLQGE
                     SLLPLANGEAEEVRDTIFAEKTYHVAYEPERCVRTHRWKYIRRFGERTRPVLPNTDDG
                     PSKELLLSYGWGEREIPPEQLYDLVLDPNEVYNLAADPTHAGTLGELRERLDAWMVET
                     KDPLLDGDVPAPKRAELNDPDGLSPNEPTTVV"
     gene            1867..2442
                     /gene="cysC"
                     /locus_tag="VHM69_10050"
     CDS             1867..2442
                     /gene="cysC"
                     /locus_tag="VHM69_10050"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2740781.1"
                     /translation="MNEERGFTLWFTGLSGSGKTTISEIVEHELRERERHVEVLDGDI
                     VRTNLSKGLTFSRDDRNINVLRIGFVANLLTRNGVGVIVSAISPYKEARDQVRRRIVD
                     FVEVFVDAPLEVCAERDVKGLYKKAYAGEIEQFTGVSDPYEPPAAPDLVLKTDEESPQ
                     ESARKVIEKLEFFGYLWPREARDTEYNWSSR"
BASE COUNT          495 a          800 c          759 g          426 t
ORIGIN      
        1 cttcgtacgc ctggcgcgac gcacgcccag gctgaagagc gttcctcccc gcgatgtgcc
       61 cgcctcggtc ctgcccaacc tgcgacggtt cgtctggatc catcgcgcgg acaccacccg
      121 ccaggcggtc tccctgtgga aggcgctcca gacccaacaa tggcgcaggg actccgatga
      181 agagctcggc gggcggggtc tgcgtttcag cttcgccgcc gttgaccatt taaagctgcg
      241 catagacgag cacaacgacg cctggcagaa cttcttcgaa gagtgtggcg tggagcctat
      301 ggaggttgtc tacgaggagt tagtcgaaga ctatgagggg actgtcttgt ggcttcttga
      361 cggtatcggg gtctctatcc ctgagaattt cggcgtcacc gagcctaaaa tgaggcggca
      421 ggcggacgat ctctcagagg agtgggtccg tctgtacaac gagcgcgcag ccgtgaaaag
      481 cgcccagaaa ggataagagt tgccggatct tccgaacatc ctctacatcc actcccacga
      541 cacggggcgg tgggtcaggc cctacggcta ctcggtaagg acgccgaaca tccagcgtct
      601 cgccgaggag ggtgtgctct tccgcaaggc gttctgcgcc gccccgacgt gttcggggag
      661 ccgggcttgc ctgctcactg gccagtacgc gcactccaac gggatggtcg gcctcgccca
      721 ccgagggttc tcgctcaagg attacaggca ccacatcgtc catacactgc gagatctcgg
      781 ttactggtcg gcgctaatcg gcgagcagca catctccaag aagcctgacg tcatcggcta
      841 cgacgaggtc atcaagatca gcaccagcca caccgacgac gtcgtgccgg tgaccctcga
      901 ccttctggaa cgcgcgagcg acaggccatt cttcctctcg gtcggtttct tcgagaccca
      961 ccgcgaatgg ttccgcccaa gctccccgaa gaaggccaac tacgtcatac ctcctcccaa
     1021 tttgcccgac gcacccgaga cgcgcctgga catggccgcc ttccaggaga gcgcccgctc
     1081 gctcgacagg ggcataggcg ccgtcctcga cgccctcgat gcggaggggc tggccgacga
     1141 cacgctcgtg atctgcacga cggaccacgg cctcgccttc cccggcgcga aagctaccct
     1201 ctacgaccgg gcctcggcgt catgctcatc ctgcgcgggc caggcggttt caccggtggc
     1261 agggcgagcg acgccctcgt ctcccacata gacctctttc cgaccgtctg tgacctcatc
     1321 ggtgtcgaga gaccggagtg gctgcagggc gagtcgctcc taccgctcgc gaacggggag
     1381 gccgaggagg tcagggacac catcttcgcc gagaagacct accacgtcgc ctacgaaccc
     1441 gagcggtgcg tccgcaccca ccgctggaag tacatccggc gcttcggaga gcgcaccagg
     1501 cccgtcctgc cgaacaccga cgacggcccg agcaaggagt tgctgctctc ttatggatgg
     1561 ggagagcgcg agatcccccc agagcaactc tacgatctgg tcctcgatcc caacgaggta
     1621 tacaacctcg ccgcagatcc cacccacgcg gggacgctcg gggagttgcg tgaaagacta
     1681 gatgcctgga tggtcgagac gaaagaccct ctcctcgacg gggacgtgcc tgcgccgaag
     1741 cgcgccgagc tcaacgaccc tgacgggctc tcccccaacg agcccacgac cgtcgtgtga
     1801 gcggttctca aacgggtatg taaactaagg acagaagaga tcatcaatct accgaaagga
     1861 acgtatatga acgaagagcg cggattcacg ttgtggttca ccggtctctc cgggtcgggg
     1921 aagaccacca tctccgagat cgtcgagcac gagctgcgcg aaagggaacg gcacgttgag
     1981 gtcctcgacg gggacatcgt gaggaccaac ctgtccaagg gcctcacctt ctcgagagat
     2041 gacaggaaca taaacgtgct gcggatcggt ttcgtggcga atttgcttac gcgcaacggg
     2101 gtcggtgtga tcgtcagcgc catctctcct tacaaggagg cgcgggacca ggtgcggcgc
     2161 aggatcgtgg acttcgtcga ggtcttcgta gacgctccac tcgaagtttg cgccgagcgg
     2221 gacgtgaagg gcctgtacaa gaaggcttac gctggtgaga tagagcagtt cacgggcgtc
     2281 tccgaccctt acgagccccc tgccgccccc gacctcgtcc tcaagaccga cgaggagtcg
     2341 ccacaggaga gcgcccgcaa ggtcattgag aaactcgagt tcttcgggta cctgtggccg
     2401 cgagaggcgc gggacacgga gtacaactgg agctcccgct gagggtcagt cccgaccgga
     2461 attctcccct ttgaacgccg
//
LOCUS       DATAIR010000412         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_303379, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000412 DATAIR010000000
VERSION     DATAIR010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303379"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VHM69_10055"
     CDS             complement(<1..1261)
                     /locus_tag="VHM69_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2740782.1"
                     /translation="MDRSKLDPVTLSVLASALAGIAEEMGAVLIRGSYSSNIKERRDC
                     STALFDAKGLMVAQAEHIPVHLGAMPEAVAAIMRRDPEPGDVFAINDPYSGGTHLPDI
                     TLVSPVAHEGEIVGYAVTRAHHSDVGGMRPGSMPSDSREIFQEGIIIPPVRLVRDGEY
                     VADVMDLLLANVRTPGLRRGDLRAQIAANNIAEERLAELIDRRGKDTVLAAFDEVVSY
                     AEKRTREAIRDLPDGEHSAEDCMEGDGITGDDIPIKATVTVEGDSMKIDFTGTADSVR
                     GNINCPLPVTRSASYFALRVLLPKDILANAGTYGPLTIEAPEGSLVNATYPSAVVAGN
                     VETSNRIVDTVLAALSGFAPRGIPAQGQGTMNNTIIGGSGWTYYETVGGGQGASHRGP
                     GPSGVHIGMSNTLNTPTEAFEMEYPMRV"
     gene            complement(1254..>2043)
                     /locus_tag="VHM69_10060"
     CDS             complement(1254..>2043)
                     /locus_tag="VHM69_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2740783.1"
                     /translation="ELGLAAEDVALGVVRVANAEMTRALRIISVERGLDPRDFALLAF
                     GGAGGMHACALAEELGMQTVLVPRAGGVLSALGLAISDIRRDYVSPYLSPLDEADAGE
                     VEKRFARMQSMAERELDDPGHTRRADLRYRGQSFELTVEADSLEKLEDRFHAAHERRY
                     GYRMQEEAVELVNLRLISTVPVEKPELDEPEPTDEATAGVREANFDGEWMEVPVFDRD
                     KMGQGSEVAGPAIVEFRESTCVVRPGWSGKVDAIGTLVLEKSDG"
BASE COUNT          315 a          667 c          685 g          376 t
ORIGIN      
        1 cgacacgcat cgggtactcc atctcgaagg cctcggtggg agtgttgaga gtattgctca
       61 tcccgatgtg tacgccggag ggaccaggac ccctgtggct cgccccctgg ccgccgccga
      121 cggtctcgta gtaagtccag ccgctgcccc cgattatcgt gttgttcatc gtcccctggc
      181 cctgcgccgg tatcccccgc ggcgcaaagc cagagagcgc ggcgagcacg gtgtcgacga
      241 ttcggttgga ggtctcgacg ttcccggcga cgaccgcgga cgggtaggtc gcgttgacga
      301 ggctgccctc gggggcttct atggtgaggg gtccgtaggt gccggcgttg gcgaggatgt
      361 ctttggggag caggactcga agggcgaagt agctcgccga gcgggttacg gggagggggc
      421 agtttatgtt gccgcgaacg gagtcggctg tgcccgtgaa gtcaatcttc atcgagtcac
      481 cctcaactgt caccgtggcc tttatgggaa tgtcatcgcc ggtgatgccg tcgccctcca
      541 tgcagtcttc ggcggagtgc tccccgtcgg ggaggtcgcg gatggcctca cgggtgcgct
      601 tctcggcgta ggagacgacc tcatcgaagg cggcgagaac ggtgtccttg ccgcggcgat
      661 ctataagctc ggcaaggcgc tcttccgcga tgttgttcgc ggcgatctgg gcccgcaggt
      721 cacctcgcct gaggccgggt gtacggacgt tggcgaggag gaggtccatc acgtcggcca
      781 cgtactcgcc gtcgcgcacc agacgcacgg gagggatgat gatgccctcc tggaagatct
      841 cacgcgagtc gctcggcatg gagccgggac gcattcctcc cacgtcggag tggtgggccc
      901 tcgtcaccgc gtaaccgacg atctctccct cgtgggcgac gggcgagacg agcgttatgt
      961 cgggcagatg cgtgccgcca gagtacgggt cgtttatggc gaagacgtcg cccggctcag
     1021 ggtcgcggcg cattatggcc gccacagcct ccggcatcgc cccgaggtgg acggggatat
     1081 gctcggcctg ggcgaccatc aaacccttcg cgtcgaagag cgccgtcgag cagtcgcgcc
     1141 gctccttgat gttcgatgag taggagcccc ggatcaggac tgcccccatc tcctcggcta
     1201 tgcccgcaag ggcgctcgcg agcaccgaga gggttacggg gtcgagcttt gatctatcca
     1261 tcactcttct ccagtacgag cgtccctatg gcgtccactt tgccggacca gcctggccgc
     1321 accacgcaag tcgactccct gaactcgact atggcaggac ccgctacttc tgaaccctgt
     1381 cccatcttat cgcggtcgaa cacgggcacc tccatccact ccccgtcgaa gttcgcctcc
     1441 cgcactccgg cggtcgcctc gtctgtgggc tcgggctcgt cgagctcggg cttctcgacg
     1501 gggaccgtgg agatgagacg caggttcacg agttcgacgg cctcctcctg cattcggtag
     1561 ccgtaacgtc gctcgtgtgc ggcatggaag cggtcctcca acttctcgag agagtcagcc
     1621 tccacggtca actcgaatga ctgtcccctg tagcgcaggt cggcccggcg ggtgtggcct
     1681 gggtcgtcga gttctctctc cgccatgctc tgcatgcgcg cgaacctctt ctcgacctct
     1741 ccggcgtccg cctcgtcaag aggggagagg tacgggctca cgtagtcgcg gcgtatgtcc
     1801 gagatggcga ggccgagggc ggagaggacg cctcccgcgc gcgggacgag gacggtctgc
     1861 atgccgagct cctccgcgag ggcgcaggcg tgcataccgc cagccccgcc gaaggcgaga
     1921 agggcgaagt ccctcgggtc gaggccgcgc tcgacgctga tgatcctgag cgcccgcgtc
     1981 atctcggcgt tcgccacgcg cacgacgccg agcgccacgt cctcggccgc gagaccaagc
     2041 tcc
//
LOCUS       DATAIR010000413         6212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_334668, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000413 DATAIR010000000
VERSION     DATAIR010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334668"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VHM69_10065"
     CDS             <1..336
                     /locus_tag="VHM69_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740784.1"
                     /translation="TTSFIPDWQVRLDPGKQRTSWYLRMWPPQRGADALGSLMRVEAP
                     RDTEPEQVDEISRWILAERAPLAKPDPRWPAMIYPIQYVEKILKPLVQGSERAYARLE
                     RQLASNGRN"
     gene            337..2397
                     /locus_tag="VHM69_10070"
     CDS             337..2397
                     /locus_tag="VHM69_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740785.1"
                     /translation="MLHEDFLDLISGPIGRVVTSEESPATAHEFFFWTAETEAAQNLD
                     IGHIVAAESEDATAVAVLDDPRRYSDLQSFLDDFYAYDGDPSLEALSERVEILVFRAR
                     VLATKHRDERKKSKRPVRTGPVYFATNAAIEFALGAEDFSGHRIPMLLHENGNERNGS
                     PQRTPLYVDEEYLLGPEAGHLNITGMSGLSTKTSHALFTIASTFQTVEDKKVAALMFN
                     VKGADLLFLDKPVEVDPADDPELAERYEKAGQRGLPSEDREMYDSLGIEVKPFDNLKV
                     FAPLRYGMESGERIVHAEGIPARKLNTLRSARGEDACVFPVIWELGDVLPYAGYVFEP
                     SDMDDKFRGFIEELRDRGVRTTADFYALLDDIEDYFEAAREEGKNVSSWNGHNHMTIA
                     KVRNRFKVLPNKCGGLLAHGRVEHGELPRADGPFEDSEMRVIDISQLTGVPQDLIVTS
                     VISKIWELAETGRLGVDKLIIFVDELNKYAPSGNSRTSSLKDTLVDISARGRHLNLTL
                     FGAQQFRSKVDDEVIGNAATSLYGRIGDEELTNSSYRSFSGTTREELLQLEKGRLLLR
                     HAHYAVPVFGRFPRPPVLMGKQGTDIFGEQRGDAASSVLAVMRNLTNNPPAIAAIRTE
                     IEGVPEEQVYEALDAVQAAHRTGHGAADPYKNFRWNLKRPARTGSGRRDASRILSGLD
                     RMRD"
     gene            2422..2655
                     /locus_tag="VHM69_10075"
     CDS             2422..2655
                     /locus_tag="VHM69_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740786.1"
                     /translation="MDFEIVGEISEVETIVVGSSIREIARLRRAYGSGRWRRLKGVAR
                     VQLRDGKIRQAEIHWYEAYGVGKREFKLKRYLD"
     gene            2660..2893
                     /locus_tag="VHM69_10080"
     CDS             2660..2893
                     /locus_tag="VHM69_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740787.1"
                     /translation="MVEKEQTIKFAVCINNDDYPASLELHKIYRVLPDEDAESDGDLR
                     VVDESGEDYLYPAQRFILVELPQAVEKSLLQNV"
     gene            3039..3263
                     /locus_tag="VHM69_10085"
     CDS             3039..3263
                     /locus_tag="VHM69_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740788.1"
                     /translation="MNDNRIEAYWQSYLATLPADSPVRDERFVVERWGDSSRMADELG
                     ALISNGTKTETCSALWEYEAEGEPLPEVGV"
     gene            3273..3506
                     /locus_tag="VHM69_10090"
     CDS             3273..3506
                     /locus_tag="VHM69_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX2740789.1"
                     /translation="MDGNGNPLCIVETTEVEVRPYDEVDAQFAYEEGEGDRSLDYWRE
                     AHWRFFSRTLLNVGREPTSDMPLVCERFHIIYR"
     gene            3799..4026
                     /locus_tag="VHM69_10095"
     CDS             3799..4026
                     /locus_tag="VHM69_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2740790.1"
                     /translation="MDYRERITIDPDVSGGKPCIRGMRITVYDVLVYLASGMSQEEIL
                     ADFPYLEAEDIRASLAFAADFERRFVAEPSL"
     gene            4112..4396
                     /locus_tag="VHM69_10100"
     CDS             4112..4396
                     /locus_tag="VHM69_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX2740791.1"
                     /translation="MDVRFYVDPETDEPHIYGHRVAEEEVVEVLHGPGEDRPGREGSR
                     IAIGRTPEGRYLRVIYVPDPEPDSVFVITAYELTGKPLVAYRRRRRQRGT"
     gene            4438..4599
                     /locus_tag="VHM69_10105"
     CDS             4438..4599
                     /locus_tag="VHM69_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740792.1"
                     /translation="MRKVLRHYEEQTEEEATAEDEAAFENREQTFMEVPNDLVPEVRS
                     LIAKHQTRA"
     gene            4680..5822
                     /locus_tag="VHM69_10110"
     CDS             4680..5822
                     /locus_tag="VHM69_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX2740793.1"
                     /translation="MSDTHLGYSRYAKLHPETSRNQREVDVQEAYGRAVDAILERDVD
                     LVIHSGDVFDSVRPATHVIIGFLKQTFRITREDIPYLVAAGNHETPRLRSTTAALEYA
                     NLVNAISAHGFDVEYYFEEVGDAGVGVTLVPHGAVFGTGAVTPSRDADVNILVTHGMV
                     PGLEARQYEMGEANLQPGMLEGGFDYIALGHYHEFHEHKPNAFYAGATERFGFGEVES
                     KPGFAIVEFDGGGLMGVEHVEIETRPMMDLKKISARDMDATELTDAVRERTSGVDLDG
                     AIVRLRAYDVRRGVASGMDRELLRDLQRRCLNFSLEVHAEERPEDVERNGSSTAVFGP
                     LGEEFATFVAERKARGELEEKFAEEFLEKGRGYLTRAASEEPESAA"
     gene            5819..>6212
                     /locus_tag="VHM69_10115"
     CDS             5819..>6212
                     /locus_tag="VHM69_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2740794.1"
                     /translation="MILERLFIENYKQLRDPLELYPPEGAIGIVGPNGAGKSTLFESI
                     LWAFFGSRGGGPRFANESIPWSGGTTKDPTTVEVTLATEGGSYTVRRRLKSGSTTAEA
                     LDAAGRTVVSGTADVTRWVEENLLRMDRT"
BASE COUNT         1359 a         1794 c         1969 g         1090 t
ORIGIN      
        1 acgacgagct tcatcccgga ctggcaggtt aggctagacc caggcaagca acgtacctcc
       61 tggtacctga ggatgtggcc gccgcagcgc ggtgcggacg ctctaggttc gctcatgcgc
      121 gtcgaggctc cgcgcgatac cgagcccgag caggtagatg agatttcgcg ttggatcctc
      181 gccgagcggg cgcctttagc aaagccagac ccgaggtggc cagcgatgat ctaccccata
      241 cagtacgtcg agaagatcct caaacccctg gtgcagggct ccgagagagc ctatgcacgg
      301 ctggagcgcc aactggcatc gaacgggagg aactagatgc tgcacgaaga tttcctggac
      361 ctgatctcag gcccaatcgg cagggtcgtg accagcgagg agagcccggc cacggcccac
      421 gagttctttt tctggacagc ggagacagaa gcggcccaga acctggatat cgggcacatc
      481 gtcgccgccg agtccgaaga cgccaccgcc gtcgccgtcc tcgacgaccc gcgacgctac
      541 tccgacctcc agagcttcct cgacgacttc tacgcctacg atggcgaccc ttcactcgaa
      601 gcactctccg agcgagtgga gatcctcgtc ttccgcgcac gagtcctcgc cacgaagcac
      661 cgcgacgaga ggaagaagtc caagcgtccg gtgcgcacag gcccggtcta cttcgccacg
      721 aacgcggcca tcgagttcgc cctgggggcc gaggacttct ccggccaccg catcccgatg
      781 ctcctgcacg agaacggcaa cgagaggaac gggagcccgc agcgcacgcc tctgtacgtt
      841 gatgaggagt accttctcgg tcccgaggcg ggccatctca acatcacggg gatgagcggg
      901 ctctcgacca agaccagcca cgccctcttc accatcgcga gcaccttcca gaccgtggag
      961 gacaagaaag tcgcggccct catgttcaac gtgaagggcg ccgacctcct gttcctggac
     1021 aagcctgtcg aggtagatcc agcggacgac cccgaactcg cggagcgcta cgagaaggcc
     1081 gggcagagag gattgccgtc cgaggatcgg gagatgtacg attctttagg catcgaggtc
     1141 aagcccttcg acaatctgaa ggtcttcgct ccgttgcgtt acgggatgga gtcgggggag
     1201 cgcatcgtgc acgccgaggg catccctgct cgcaagctga atactttgag gagcgcgcgc
     1261 ggggaagatg cttgtgtttt tcctgtgatc tgggagctcg gggacgtgct gccttacgcc
     1321 ggctatgtct tcgagccatc ggacatggac gacaagttcc gcggctttat agaggagctt
     1381 cgcgacaggg gagtcaggac gacggccgac ttctacgcgc tgctcgacga catcgaagac
     1441 tacttcgagg ctgcccgcga ggaagggaaa aacgtctcat cctggaacgg acacaaccac
     1501 atgaccatcg ccaaggtccg caatcgcttc aaggtgctcc caaacaagtg cggcggactc
     1561 ctcgcgcacg ggcgagtcga gcacggcgag ttgccgcgcg ccgacgggcc gttcgaggac
     1621 agcgagatgc gggtcattga catctcccag ctcaccgggg taccccagga cctcatagtg
     1681 accagtgtca tctcgaagat ctgggagctc gccgagacag gcaggctcgg ggtggacaag
     1741 ctcatcatct tcgttgacga gctcaacaag tacgccccct cgggcaactc cagaacctcg
     1801 tctctcaaag acacgctggt agatatttcg gcgagaggac gacacctcaa cctcacgctc
     1861 ttcggggcgc agcagttcag gagcaaagtc gacgacgagg tcatagggaa cgccgcgacc
     1921 agcctctacg gacgcatcgg cgacgaggag ctcaccaact ccagctaccg ctccttctcg
     1981 ggaaccactc gcgaagaact cctccagcta gaaaagggcc gtctcctcct caggcacgcc
     2041 cactacgccg tccccgtatt cggccgcttc ccgcgcccgc ccgtcctcat gggcaagcag
     2101 ggcacggaca tcttcggcga gcaacgcggg gacgcggcat cctcagttct cgccgtcatg
     2161 aggaatctga ccaacaaccc cccagccatc gccgcaatcc ggaccgagat agagggcgtc
     2221 ccggaggagc aggtctacga ggccctcgac gccgtgcagg cggcccaccg caccggccac
     2281 ggcgccgccg atccatacaa gaacttccgc tggaacctca agcgccccgc gcggaccggg
     2341 tcaggccgtc gcgacgcttc caggatcctt tccggcctgg atcggatgag ggactagcga
     2401 gaacagactc gcacgactgc gatggacttc gagatagtcg gggagatctc cgaggtcgaa
     2461 accattgtgg ttggtagcag catcagagaa attgcgagat tgcgcagggc ctacggcagc
     2521 ggcaggtggc ggaggctcaa aggcgtagcg cgggtgcaat tgcgcgatgg taaaatccgg
     2581 caggctgaga tccactggta cgaagcgtac ggtgtaggta agagggaatt caaactaaag
     2641 cggtatctgg actagagaca tggtagagaa agaacagacc atcaagttcg ccgtctgcat
     2701 caataacgat gactatccgg catcccttga gctacataag atctaccgtg tgctacccga
     2761 cgaggatgcg gagtcggacg gcgacctgcg agtcgtagac gaaagcggcg aagattacct
     2821 ttatccagcg cagcgcttca ttctcgtgga actgccacag gcggttgaga agtcactact
     2881 gcagaatgtc tagagatggc gaattggtaa cgaacacaag gagttcatcc ggtcgccgcg
     2941 acacgtccag gaagttcact ggcctgggtc ggatgaagga ttaactctgg ccagggtccc
     3001 gcacgtctag ggtacgcggc ttctcactgg agcaggtaat gaacgataac cgcatcgagg
     3061 catactggca aagctatctg gcgaccctcc cggcggattc gccggttcgc gatgagcgat
     3121 tcgtggtgga gagatggggc gactcttctc ggatggcaga cgagctaggt gccctgatct
     3181 ccaacgggac caagacggag acctgctccg cgctctggga gtacgaagca gaaggggagc
     3241 ccttgcccga ggttggagtg tagacgatcg tcatggacgg aaacggtaac cctctgtgca
     3301 tcgtcgagac gaccgaggta gaggtgcgcc cctacgacga agttgacgcc cagttcgcct
     3361 acgaagaagg agaaggtgat cgctcacttg actactggcg ggaggcacac tggcgcttct
     3421 tttcgcggac gctgctgaac gtaggcagag agccaacttc agatatgccg cttgtatgcg
     3481 agcggtttca tataatttac agatagtttg caggtaatat aaatgcgaca gattatggag
     3541 acgcgagaga tcaccatacg ggttgacgca gaggccgcaa atgcttacgc ggcggcttca
     3601 cccgaggagc gtgagaagat aaatctgctt ctcagtctac gattgagtca ggtcactggt
     3661 tcttcgcctc cgctcgaaca ggttatgcga gagatttccg aagcggcaca caatcgaggt
     3721 ctgacagaga acttacagca ttgaacgtgc gcgtgtttgc ttcttgaacc agcccatcct
     3781 gcaacgtaga cttctggggt ggactacaga gagcgcatca ctattgaccc tgacgtaagt
     3841 ggcgggaaac cgtgcatcag ggggatgagg atcaccgtct atgatgtcct cgtttacctc
     3901 gcgtccggta tgtcgcagga ggagatcctg gccgacttcc cctatctgga ggcggaagat
     3961 atccgcgcca gcctggcttt cgccgccgat ttcgagcgca ggttcgtggc agaaccttct
     4021 ctttgaggct gctcttcgcc gagagtctct gtccacatct ggtccgtatg ttagcgggag
     4081 tgtcacgggc gatcaggtat catgagtatg gatggacgtg cgtttctacg tggacccgga
     4141 gaccgatgaa ccacacatct acggtcatcg ggttgccgag gaagaagtgg tcgaggtgct
     4201 ccacggtccg ggcgaagacc gtccgggaag agaaggatca cgtatcgcta tcggccggac
     4261 acccgaaggc agatacttgc gggttatcta cgtgccggat cccgagccgg atagcgtttt
     4321 cgtgatcacc gcgtatgaat tgactggcaa gcctctggta gcctaccgga gacgccgtcg
     4381 tcagagagga acatgatgag ccagactgaa tttcctccag gttgggacga agagagagtg
     4441 cggaaggtcc ttaggcacta cgaggagcag accgaagaag aagcgaccgc cgaagacgaa
     4501 gcagccttcg agaaccggga gcagactttc atggaagtcc cgaacgacct cgtgcctgag
     4561 gtccggtcgc taattgccaa acatcaaaca cgagcttgag gtccaagcga atccactaca
     4621 ggatgcggcc gaaatagcgt aggctcgaaa agaggaatga gatcgtaagg atcgcacata
     4681 tttcagatac tcatttggga tacagcaggt acgcgaaact ccatcctgag acgagccgca
     4741 accagcggga ggttgacgtt caggaggctt acgggcgtgc tgttgacgct atcctggagc
     4801 gtgatgttga cctggtgatc cactccggcg acgtcttcga ctcggtgcgt cctgcgacgc
     4861 atgtgatcat cggcttcctg aaacagacat tccgtatcac acgagaggac attccctatc
     4921 tcgtcgccgc aggcaatcac gagacgccgc gcctgcgctc gacgacagcg gccctcgaat
     4981 acgcgaacct ggtaaacgcg atctccgccc acggcttcga tgtcgagtac tacttcgaag
     5041 aggtcggcga cgccggcgtc ggggtgactc ttgtgccgca cggggcggtg ttcggaacgg
     5101 gggccgtgac gccttctcgc gacgcggacg tgaacattct ggtcacgcac gggatggtgc
     5161 ccggtctgga agctaggcag tacgagatgg gcgaggcgaa cctgcagccg gggatgctcg
     5221 agggcggctt cgactacatc gcactcggcc actaccacga gttccacgaa cacaagccga
     5281 acgccttcta cgcgggggcg acggagcggt tcgggttcgg ggaggtcgag tcgaaaccag
     5341 gtttcgcaat cgtggagttc gacggcggcg gacttatggg cgtcgagcac gtcgagatag
     5401 agacgcgtcc gatgatggac ctgaagaaga tcagtgcccg cgacatggac gcaacggagc
     5461 ttacggacgc tgtgagggag cgaacctcgg gcgtcgacct ggacggcgcg atcgtacgcc
     5521 tccgcgcata cgacgtgcgc cgtggcgtgg cgagcgggat ggaccgcgag ttgctgcggg
     5581 atttgcagcg gaggtgcctg aacttctccc tggaggtcca cgccgaggaa cgaccggagg
     5641 atgtcgagag gaacggctcc tcgaccgccg tattcggacc tctcggtgag gagttcgcga
     5701 cgttcgtcgc ggagcggaaa gcgcgcggcg aactggagga gaagttcgct gaggagttcc
     5761 tggagaaggg acgcggatac ctgacgcggg cggcgagcga agagccggag agcgccgcgt
     5821 gatcctggaa cgcctcttca tcgagaacta caagcaactc agagatcccc tggagctgta
     5881 tccgcctgag ggagcgatcg ggatcgtcgg gccgaacggc gcgggcaaat ccaccctctt
     5941 cgagagcata ctctgggcat tcttcggctc acgtggcggg ggcccgcgct tcgcgaacga
     6001 gtccatcccc tggagcggcg gaacgacgaa ggacccgacg acggtcgagg tgactctggc
     6061 gacggaagga ggatcttata ctgtccggcg gcgtctcaag agcggttcga caactgcaga
     6121 ggcgctcgac gccgctggta ggacggtcgt cagcgggaca gccgacgtca cgcgctgggt
     6181 cgaggagaac ctgctgagga tggacaggac gg
//
LOCUS       DATAIR010000414         7839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_303496, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000414 DATAIR010000000
VERSION     DATAIR010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303496"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1423
                     /gene="murC"
                     /locus_tag="VHM69_10120"
     CDS             65..1423
                     /gene="murC"
                     /locus_tag="VHM69_10120"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX2740795.1"
                     /translation="MKIHMIGIGGAGMSGIAEVLKRRGHDVTGSDLKDSPYTRRLREA
                     GITVYIGHASSQVGDADQVVISTAIPKTNPELLEARRRSIPIIPRAAALAWILEGSRS
                     VAVAGTHGKTTTTSMTAHILKALGENPTALVGGELNDIGSNVAFGRSDLVVAEADESD
                     RSFLYLRPQAAVVTNVEFDHPDFYSSLDDVVATFEQFVRSLPEDGHLVLCADDASCLK
                     VAAEAHCPVTSYGLDSGTLRAEILSPSSYVLIEDDERRGEIELGVYGRHNVLNSLAAA
                     AVARWLGYDAYEAARTLKDFGGVRRRFQLKGERADVRVVDDYAHHPTELSATLAAARS
                     TMNHDGRVIAVFQPHRYSRTRALYREFGESFSSADAVLVTEVYGAGEMPQPGVNGKLI
                     VDAICETRGHPEVYYIPQQDTIPRVLQAVSRKGDLVLTLGAGDISKAGEDLLDMLEER
                     GP"
     gene            1459..2097
                     /locus_tag="VHM69_10125"
     CDS             1459..2097
                     /locus_tag="VHM69_10125"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2740796.1"
                     /translation="MAVATTLTVVLVTYFSFPVTGIKVEGALMYPESEAWDAVPDHAS
                     LLTLNKEELKRKVQSNPWVQGVKVYENWKSGIVTVQVEERHAVLDAEVDGRRIILSND
                     GRELPGLGGAGLDRVELDRDQVGEILEFARVLNASGVRLDSVEGAGAGGIEATVDGRT
                     VIFSGDVDESQARALKDIMPRHPDAQLFDLRSPERVVVGAPAPDETNSGSRG"
     gene            2104..3306
                     /gene="ftsA"
                     /locus_tag="VHM69_10130"
     CDS             2104..3306
                     /gene="ftsA"
                     /locus_tag="VHM69_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX2740797.1"
                     /translation="MAQSLVYGIDVGTTKIVALAGRVDPRRGWVEVLSMGEAPSYGLK
                     RGVVVDRQAAADSIAEAMEACGVRGGKVTVGIAGSHISSLNTEVTLLNRSRNLTVTDR
                     FVRKLEQEARQLDLDEDERVIHVVPRGYVLDGSEGVKNPAGLAARKVTMRAHVVSGAV
                     SSIQNLLRAVEDCGVRVSKVVLEPLASSEACLLEADRESGVVLVDIGGGTTDIATFYR
                     GALTHTAVIPIGGESFTSDVAYGLKLPQHKAEELKLRYGTVLSRTVDSVAAVKLDDRH
                     YNAHFVSQILEYRAREVYDHVRDSLDKTGMRDLVPGGIVLTGGGSLLEGMTELAEDTL
                     GMRARTAIPRRVRGEVKPVQKPQYATSVGLLYFSARNDDPRPKGKGAGASSFGSIVEA
                     VKGWFRGR"
     gene            3333..4424
                     /gene="ftsZ"
                     /locus_tag="VHM69_10135"
     CDS             3333..4424
                     /gene="ftsZ"
                     /locus_tag="VHM69_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2740798.1"
                     /translation="MEGWNGMLDAATNYLAVIKVVGIGGGGTNAVNRMINSGLQGVEF
                     IAINTDAQALQMCDADQKIHIGEKITRGLGAGADPKIGMEAAEENKAEIEEALRGADM
                     VFVTAGKGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRSTYAEEGIKRLKE
                     NVDSLIIIPNDRLLQVAEKRTSMMDAFKMADDILRKGVQGITDLITVPGLINLDFADV
                     RTIMHDSGSALMGIGESSSETRGAEAAKTAISSPLLEASIEGATGIILNITGGPDLGL
                     FEVNEAAEIVHNAAHQDANLIFGAVIDESNGDRVSVTVIATGFDQRLANQRRSERSVA
                     EPAQQHQEEPPSSQESGDVLDIPAFLRRR"
     gene            4440..5195
                     /locus_tag="VHM69_10140"
     CDS             4440..5195
                     /locus_tag="VHM69_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX2740799.1"
                     /translation="MRRAHTQAEYGASVHTAEGGAVYVAPYPEPEEARIRFFTRLGGV
                     SGPPYDSLNVSKVVGDAPDAVDENLSIIREAMGERPAAWVRQVAGDVVLRVSEPGLAG
                     EADALITREPDLCLSVAVADCVPVALVGGREVGMVHSGWRGTLAGVSGKAAREMGTSP
                     VRAYIGPCIRRCCYEVSEELAQMFALEFGDGVVSGRHLSLPDAIGADLGRAGVEVNDL
                     GLCSGCGPDLFYSHRMQGPLTGRNLASVVMEAR"
     gene            5192..5920
                     /locus_tag="VHM69_10145"
     CDS             5192..5920
                     /locus_tag="VHM69_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX2740800.1"
                     /translation="MSGQLTATDVRERLTGVRENIDDALQRSGRGNEQVRVLVATKYY
                     TIEQLAALADAGVDLVGENRAEDLIDKQERFLDSFEWHFIGHVQRRKARVVVPRVTLV
                     HSVDSVRLIEEVAKRAPDGGTGVLIQVNVADEESKYGVSEEEVEVLLEAAAGTGGKVK
                     ARGFMTLAPLVKDPEDVRYVFAKLRTIRDRLRKSWSPHFDLSELSMGMSGDYEVAVEE
                     GATVVRIGRMLIEEDGPVRRQDGS"
     gene            5910..6485
                     /locus_tag="VHM69_10150"
     CDS             5910..6485
                     /locus_tag="VHM69_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX2740801.1"
                     /translation="MGVRDRLDRIAAWFGFGVDDEDYYEDEEEEDRRYGNESYVNGAR
                     GAEPGPTVRRLGRAERSSAFGTSLGDLFGSEGSGRGERYGSQNPPAHLRPVPDQRTTR
                     VSVLEPSSFNDAQNLADRFKRQQPVILNLQNVDGDLSRRMVDFCSGLTYALDGNIQSV
                     ANRVFLLTPRDVEVSAEERKRIAERAFFNQL"
     gene            6504..7289
                     /gene="proC"
                     /locus_tag="VHM69_10155"
     CDS             6504..7289
                     /gene="proC"
                     /locus_tag="VHM69_10155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX2740802.1"
                     /translation="MKKVGIIGAGKIGGSLLLRLAESGGFDLGVSDIHLEQLRLFKES
                     GVRTTSSNRELAESSDLVIVAVKPWDVAQVMQDISPAFEDTNRAVTSVAAGVSIARIS
                     ENLPEGTAVLRVMPNVCASVGLGSAVVTANEPGRAALPTVMDIFQHVGDVLELPERLF
                     DAATALHGSGPAYVALFADALIQAGVREGIPRDVARRLVIGTIGGTAVLLKERSAHQV
                     RDEVMTPGGTTAAAFVAMEKAGFVAAVYDGVEAATEKARGLGG"
     gene            7286..7627
                     /locus_tag="VHM69_10160"
     CDS             7286..7627
                     /locus_tag="VHM69_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2740803.1"
                     /translation="MSVLLLQDFGFSVASLLAAAVTYTYYILFGFIIASILFSWFPGY
                     PSSGVMQAIYDAVKAVTNPILWPIRSRVPMLQLGGFGIDLSPIIAIFGLSIARTLLLI
                     VIENFIQPVTG"
     gene            7634..>7839
                     /locus_tag="VHM69_10165"
     CDS             7634..>7839
                     /locus_tag="VHM69_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2740804.1"
                     /translation="MPIRPIDIRRKEFKSGFRGYDSNQVDDFLDAVADEFERNYTENQ
                     RMREEVSSLRDRLQQFEDLEGSIRA"
BASE COUNT         1545 a         2343 c         2593 g         1358 t
ORIGIN      
        1 cccccggagc ggctgccgag gtggccgaaa gactgctgcg agctggagag aaggagtgag
       61 gaccatgaag atccacatga tcggcatcgg cggggctgga atgagtggga tagccgaagt
      121 cctcaagagg agaggtcacg acgtcacggg ctccgatctc aaagactctc catatacgcg
      181 aaggttgcgc gaggccggca tcaccgtcta catcggtcac gcgtctagcc aggtaggaga
      241 cgccgaccag gtggtgatct cgaccgccat acccaagacg aacccggagc tcctggaagc
      301 ccggcgccgc tccatcccga tcattccgcg cgccgccgca ctggcgtgga tactggaggg
      361 tagccgcagc gtcgccgtcg cgggtaccca cgggaagacc accacgacga gcatgacggc
      421 gcacatcctc aaggcccttg gcgagaaccc gacggccctt gtcggcggcg agttgaacga
      481 catagggagc aacgtcgctt tcgggaggtc cgacctcgtc gtggccgagg ccgatgagag
      541 cgacaggtct tttctgtacc tcaggccgca ggccgccgtg gtcaccaacg tcgagttcga
      601 ccatcccgac ttctactcct cgctcgacga cgtggtggct accttcgagc agttcgtcag
      661 atctctgccc gaggacggcc acctggtgct ttgcgctgac gacgcgtcct gcctcaaggt
      721 agcggccgag gcacattgtc cagtgactag ttacggtctc gactccggaa ccctcagggc
      781 cgagatcctt agccccagca gctacgtgct gatcgaagat gatgaaaggc gtggggaaat
      841 cgaacttggc gtctacggtc gccacaacgt gctcaactca ctggccgccg ccgcggtggc
      901 ccgctggctc ggatacgacg cttacgaagc ggctcgcacc ctgaaagact tcggaggcgt
      961 gcgccgccgg ttccagctaa agggagagcg cgccgacgtg cgcgtcgtag acgactacgc
     1021 ccaccacccg acggagctct ctgcgacgct cgccgccgcc cgctccacca tgaatcacga
     1081 cggaagggtg atcgccgtct tccagccgca ccgttactct cgcacccgcg cgttgtacag
     1141 ggagttcggc gagtcgttct cctcggctga cgctgtgctc gtcaccgagg tatacggcgc
     1201 gggcgagatg ccccagcccg gcgtgaacgg caagctcatt gtcgatgcca tctgcgagac
     1261 gcgaggacac cctgaggtct actacatccc ccagcaggac accatcccgc gcgtcttgca
     1321 agcagtctcg aggaagggtg acctcgtgct taccctcggc gcgggagaca tctccaaggc
     1381 tggtgaagac ctgctcgaca tgctggagga gcgaggaccc tgacccactc actgcgcgcg
     1441 atcctgatct cggcggccgt ggccgtcgct actaccctca ccgtcgtcct cgtcacctac
     1501 tttagcttcc ccgttaccgg catcaaggtc gagggcgccc tcatgtaccc ggaatccgag
     1561 gcctgggacg ccgtcccaga tcatgcgagc ctcctcaccc tcaacaaaga ggagttgaaa
     1621 cgtaaggtac agtctaaccc ttgggttcag ggtgtaaagg tgtacgagaa ctggaagtcc
     1681 ggtatagtta ccgttcaggt tgaggagcgc catgcggtgc tcgacgccga agtcgatggg
     1741 cggcggataa tcctatcgaa tgacggaaga gagcttccgg ggctcggggg agcgggtctc
     1801 gatagggtgg agttggatcg ggaccaggta ggggagatct tggagttcgc gagggtgttg
     1861 aacgcaagcg gggtcaggct cgactctgtc gagggggctg gcgccggggg catcgaggcc
     1921 accgtggatg gccggaccgt gatcttctcc ggcgatgttg acgaaagtca ggccagggca
     1981 ttgaaggaca tcatgcccag gcatcctgac gcccagctct tcgacctacg ttctcccgag
     2041 agggtagtag tcggcgcgcc ggcccccgac gaaacgaata gcgggtcgag gggctgatcg
     2101 caaatggccc agtcactggt ctacggtata gacgtgggta ccaccaagat agtggcgctc
     2161 gccggccggg tagacccgcg aaggggctgg gtcgaggtgc tctcgatggg ggaggcgccg
     2221 agctacgggc tgaagcgcgg cgtcgtcgtg gaccgtcagg ccgcggcgga ttcgatcgcc
     2281 gaggccatgg aggcatgcgg cgttcgcggc ggtaaggtga ccgtcgggat cgccggtagc
     2341 cacatctcat ccttgaacac ggaggtcacc ctgctgaacc gcagcaggaa cctcacggtc
     2401 accgataggt tcgtccgcaa gctcgaacag gaggccaggc aactggacct ggacgaggat
     2461 gagcgggtga tacacgtcgt ccccaggggc tacgtcctgg atgggtccga gggcgtcaag
     2521 aaccccgccg gcctcgccgc ccgcaaagtc acgatgcgag cccacgtggt gagcggtgcc
     2581 gtatccagca tccagaacct tctgcgcgcc gtcgaggact gcggcgtcag ggtttcgaaa
     2641 gtcgtgctcg agccactggc ctcctccgag gcctgtctac ttgaggctga ccgggagtcc
     2701 ggcgtcgtac tcgtggatat cggcggcggc acaacggaca tcgccacctt ctacagggga
     2761 gccctcaccc acaccgccgt cataccaatc ggcggcgaga gcttcacctc agacgtcgcc
     2821 tacgggctca agcttcccca acacaaggcg gaggagctca agttacgcta cggcaccgtt
     2881 ctctccagaa ccgtagactc ggtggccgcc gtcaagctcg acgatcgcca ctacaacgcc
     2941 catttcgtca gccagatcct ggagtaccgc gcccgagaag tctacgatca cgtacgcgac
     3001 tctctggaca agacagggat gagagacctg gtccccggcg gcatagtcct gaccggcggc
     3061 ggctccctcc tcgaaggcat gaccgagctc gcagaagata ccctcggtat gcgcgcaaga
     3121 accgcgatac ctcgccgcgt tcgaggcgag gttaaacctg tacagaaacc tcagtatgca
     3181 acgtctgtag gtctgctata cttctcggcc agaaacgatg accctagacc aaagggtaaa
     3241 ggtgcagggg cgagtagttt tggtagcata gtggaggcgg tcaagggctg gttccgggga
     3301 aggtagaccg gcgggcccct ggagagtgtg tgatggaggg ttggaacggc atgttggacg
     3361 cggcgacaaa ctatctggcg gtcatcaagg ttgtcggcat tggcggtggg gggaccaacg
     3421 ccgtgaaccg catgatcaac tccggtctgc agggggttga gttcatagcg ataaacacgg
     3481 atgcccaggc tctccagatg tgtgatgctg accagaagat tcacattgga gagaagatca
     3541 cacgcggcct cggcgcaggc gccgacccga agatcgggat ggaagccgcc gaggagaaca
     3601 aggcggagat cgaggaggcg ctgagaggcg ctgacatggt cttcgttacc gcgggcaagg
     3661 gcggtggcac gggtactggt gccgcacccg tcgtcgcgaa gattgccagg gactcgggtg
     3721 cgctgaccgt cggggtcgtc acccggcctt tcgcgttcga gggccgcagg cgttcgacgt
     3781 acgccgagga gggcatcaag cgcctcaagg agaacgtgga ctccctgatc atcattccga
     3841 atgaccgact tttgcaggta gccgagaagc ggaccagcat gatggacgcc ttcaagatgg
     3901 ccgacgacat cctcaggaaa ggtgtacagg gcatcacaga cctcatcacc gttcccggtc
     3961 tcatcaacct cgacttcgcc gacgtgcgca cgatcatgca cgattccggg tcggcgctta
     4021 tgggtatcgg cgagtcgagc agcgagacga ggggtgctga ggccgcgaag acggcgatct
     4081 ccagccctct gctcgaggcg agtatagagg gcgctacagg aatcatattg aacatcacag
     4141 gcgggcccga cctcgggctc ttcgaggtca acgaagcagc cgagatcgtc cacaacgcgg
     4201 cgcatcagga cgctaacctt atcttcggcg cggtcatcga cgagagcaac ggcgacaggg
     4261 tgagcgttac cgtgatcgcc accggcttcg accagcgact cgcgaaccag cgccgctcgg
     4321 agcgctcggt cgccgagccg gcccaacagc accaggagga acccccttcc tcgcaagagt
     4381 cgggagacgt cctggacata ccggccttcc tcaggcgcag gtaggccagc gcccggggcg
     4441 tgcgcagggc tcatacccag gctgaatacg gcgcctccgt ccacaccgcg gaagggggcg
     4501 ccgtctacgt cgcgccgtat cccgagccgg aggaggcgcg tatccggttc ttcacccgtc
     4561 tgggcggtgt ctccgggcct ccgtacgatt cactgaacgt ctccaaagta gtcggcgacg
     4621 cgccagacgc ggtcgatgag aacctctcca taatcaggga ggcgatgggc gagaggcccg
     4681 cggcctgggt tcgccaggtc gccggggatg tcgtattgcg ggtgtctgag cccggtctcg
     4741 cgggagaggc agacgcgcta atcacccgcg agcctgacct ctgcctctcg gtggccgtcg
     4801 cggattgtgt acccgtcgcg ctcgtcggtg ggcgagaggt cgggatggtt cactcaggct
     4861 ggcgaggaac actcgcgggg gtgtcgggca aggcggcgcg agagatggga acatcgccgg
     4921 ttcgcgccta catcgggccc tgtatcaggc ggtgctgcta tgaggtgtca gaagagcttg
     4981 cacagatgtt cgctctggag ttcggggacg gcgtagtctc gggccgccac ctctcacttc
     5041 ctgacgccat cggggcagac ctcggtcggg cgggggtcga ggtaaacgac cttggactct
     5101 gcagcggttg cgggccggat ctcttctact cccatcgcat gcaggggccg ctcacgggtc
     5161 gcaacctcgc atccgtggtg atggaggctc gatgagcggg caactcaccg caacggatgt
     5221 cagggagagg ctcaccgggg tacgcgagaa tatagatgat gcgcttcaga ggagtgggag
     5281 aggcaacgag caggtgcgtg tactcgtcgc aaccaagtac tacacgatcg agcagctcgc
     5341 ggcgctcgcg gacgccggcg tcgacctcgt gggcgagaac cgcgccgagg acctcatcga
     5401 caagcaggag cgattcctgg atagcttcga gtggcacttc atcggccatg tgcaaagacg
     5461 caaggccagg gtcgtcgttc cgagggtgac cctcgttcat tccgtggatt ctgtgcgcct
     5521 gatcgaggag gtagccaaga gggcgcctga tggtggaaca ggggtactca tccaggtgaa
     5581 cgtggcggat gaggaatcca agtatggagt ctccgaagaa gaggtggaag tgttactaga
     5641 ggctgccgcg gggaccgggg gaaaggtcaa ggcaagaggg ttcatgacgc tcgctccgct
     5701 ggttaaagac cccgaagacg tacgctatgt tttcgcgaaa cttcgtacga tccgtgatag
     5761 gctacggaaa agttggagtc cgcacttcga tctctccgaa ctctccatgg gaatgagtgg
     5821 cgattatgag gtagcggtcg aagagggagc gactgtggtc aggatcgggc ggatgctaat
     5881 agaggaggat gggcccgtaa ggaggcaaga tgggagttag ggaccgtctg gaccggatcg
     5941 cggcctggtt cgggttcggt gtcgatgacg aggattatta tgaggacgaa gaggaagagg
     6001 accgccgata tgggaacgaa tcctacgtga acggtgctcg cggcgccgag ccgggaccga
     6061 ccgtgaggcg ccttggccgc gcggagcgtt cctccgcctt cggtacttcg ctcggggatc
     6121 tcttcgggag cgaagggtcg ggcaggggag agcgctacgg cagccagaac ccacccgcgc
     6181 acctgcggcc cgtccccgac cagcgcacga cacgcgtgag cgtcctcgag ccctcgagct
     6241 tcaacgacgc ccagaacctc gctgaccgct tcaaacgaca gcagcccgtg atcctgaacc
     6301 tccagaacgt tgacggcgac ctgagtcgaa ggatggtgga cttctgctcg ggcctgacct
     6361 acgcgctaga cggcaacatc cagtcggtgg ccaacagggt cttcctgctc acgccgcgcg
     6421 acgtggaggt gagcgcggag gagcgtaagc gcatcgccga gcgggcgttc ttcaaccagc
     6481 tctagaagcc tccgaggggg actttgaaga aggtcgggat aattggagcg ggaaagatcg
     6541 gaggctctct tctcctcagg ctcgccgaat ccggcgggtt cgacctcggc gtaagcgaca
     6601 tccatctgga acaactcagg ctcttcaaag agtccggcgt acgtaccacc tcctccaacc
     6661 gcgagctagc cgaatccagc gaccttgtga tagtcgccgt caaaccctgg gacgtggcgc
     6721 aggtgatgca ggatatctca cccgccttcg aggacacgaa cagggcggtc accagcgtcg
     6781 ccgcaggcgt ctctatcgcc cgaatctccg agaacctgcc tgaagggacg gcggtcctgc
     6841 gcgtgatgcc gaacgtgtgc gcttctgtgg gccttggctc cgccgtggtt actgcgaacg
     6901 agcctggacg cgccgcgctt ccgacggtta tggatatctt ccagcacgta ggggatgtgc
     6961 tggagttgcc cgagcgcctc ttcgacgcgg cgacggccct gcacggatcc ggtcccgcat
     7021 acgtcgcact cttcgctgac gccctgattc aggcgggcgt cagagaaggc atcccgcgcg
     7081 acgtcgcgcg caggctggtc atcggtacaa taggcggcac ggcggttcta ttgaaggagc
     7141 ggagcgctca ccaggtgcgc gacgaggtta tgacaccagg cggcacgacg gccgccgcgt
     7201 tcgtagcgat ggagaaggcc gggttcgtgg ccgcggtcta cgacggcgtc gaggcggcga
     7261 ccgagaaagc acgggggctc ggcggatgag cgtgcttctc ctgcaggact tcggcttcag
     7321 cgtggcgagc ctgctcgctg cggcggtcac ctacacttac tacatactct tcgggttcat
     7381 catcgcctcc atcctcttct cctggttccc ggggtatcct tcgagcggcg tcatgcaggc
     7441 catctacgac gctgtcaaag ccgttaccaa tccaatactt tggccaataa ggagccgcgt
     7501 gccgatgctg cagctcggcg ggttcgggat agacctctcg ccgatcatcg caatcttcgg
     7561 gctctctatc gcaaggacct tgcttttgat cgtcatcgaa aacttcatac aacctgtgac
     7621 ggggtgatct gacatgccta tacgaccgat agatataagg cgcaaggaat tcaagagcgg
     7681 attccgcggc tacgactcga accaggtcga cgatttcctg gacgccgtgg cggacgagtt
     7741 cgagcgcaat tacacggaga accagaggat gcgcgaggag gtgtcgagcc tccgcgacag
     7801 gctccagcag ttcgaggacc tggagggttc tatacgggc
//
LOCUS       DATAIR010000415         8179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_26197, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000415 DATAIR010000000
VERSION     DATAIR010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26197"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1300
                     /gene="sat"
                     /locus_tag="VHM69_10170"
     CDS             107..1300
                     /gene="sat"
                     /locus_tag="VHM69_10170"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX2740805.1"
                     /translation="MNVSTEYTTIAPHGGELVDRRAPEDQRDGLLGRAEGFARVTLGP
                     RALSDLEMISTGVFSPLTGFMVSEDYESVVETMHLKNGLVWSLPITLPGGEEESNGIR
                     VGEEISLTNGDGRILATMIVEDRYSYDKVHEASEVFGTTSDEHPGVAAVYRQKDVLLG
                     GEVTLLVDDPNPKPFPSYHYEPRELRKVFAEKDWKRVVGFQTRNPVHRSHEYIQKSAL
                     EIVDGLLLNPLVGETRSEDIPADVRMKSYETILDRYYPKDRTMLAVFPAAMRYGGPRE
                     AIFHAICRKNYGCTHFIVGRDHAGPGKDSSGRPFYGDYDAQHIFDDFEDGEIGLTPLF
                     FEHAFFCLECQGMGTTKTCPHSKEHHVFISGTKVREMLSKGEYPPPEFSRPEVIEVLM
                     DGQRT"
     gene            1366..1938
                     /gene="cysC"
                     /locus_tag="VHM69_10175"
     CDS             1366..1938
                     /gene="cysC"
                     /locus_tag="VHM69_10175"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2740806.1"
                     /translation="MENGMRNGRGFTLWFTGLSGAGKTTISEIVEHELRNRFRKIEVL
                     DGDIVRTNLSKGLGFSKEDRDTNVLRIGFVADLLTRNGVGVIVSAISPFKEVRDQVRR
                     NIGGDFIEVFVDAPLEVCAERDVKGLYKKAFSGEIPQFTGVSDPYEAPAAPELHIKTD
                     EEDAQASARKVIGYLEELGYLRPVEALDRV"
     gene            1935..2750
                     /locus_tag="VHM69_10180"
     CDS             1935..2750
                     /locus_tag="VHM69_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2740807.1"
                     /translation="MIDTTPALFGLLVGFLVGLTGVGGGSLMTPFLVAVMGVPATTAV
                     GTDLTFATLTKLTGSFQHFRQKSVNLEIAIFLGMGSIPAGLLGVGTLEWIKGTFDPER
                     VESIMITIIAATLVLVGASLIYRDYFMPKRDDGPKPGKWNGKTRMSNRRRMYTVLFGA
                     LGGYLVGLTSIGSGSVMAVILLLLYPIAPAVIVGTDITHATVLSFVVGIAHMTQGNVN
                     FTLAGTLLLGGIPGVLVGSRLAPWIPGKPLKLILAIMLIFVGLRLLLAQGWLW"
     gene            2898..3665
                     /locus_tag="VHM69_10185"
     CDS             2898..3665
                     /locus_tag="VHM69_10185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2740808.1"
                     /translation="MRDRIALVTGAGAGIGRAIALRLSKEGAAVVVADVDEESGAETV
                     RRIEADAGRGAFVLADVATEAGAREMVEFAGRTFGGLDVLVNNAGGAPEPHFPEAAPE
                     HWLRTLDLNLRGVMLSIHFGLMAMRRRGGGVIVNISSMAGVGYQPYHAPEYAAGKAGV
                     VRLTTSLAYLKENMGVRVNCICPGWVETEAVHEALARMTEREHAAQSMPPPDVLIQPD
                     EIADAVGMFVRDDTLAGRVMIWPDGEQRRLVPIGAMP"
     gene            complement(3673..4530)
                     /locus_tag="VHM69_10190"
     CDS             complement(3673..4530)
                     /locus_tag="VHM69_10190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740809.1"
                     /translation="MAVEGVTIGEKYDPEHVGRFFDEYGEREWERLDPDAPARVRFHL
                     HRRYLHRYVRRGDHILEAGAGAGRFTIELAELGAVVTVGDISSGQLELNRKKVTEAGC
                     EQRIVTRVSLDITDLSRFPESNFDGVVCYGGPLSYVFDRADDALAEMLRVTRPGGHVL
                     LSVMSLAGATRKFLGAIFDLYREVGMEEVQRVVDTGDQYGVVAPHGHHCHMFRWEELK
                     ALLERHSCEIVAASASNFLSVQDEAVLEEAGRDPVFWETLLEWESRFCEQSGVLDAGT
                     HIIVVLRRV"
     gene            complement(4543..5673)
                     /locus_tag="VHM69_10195"
     CDS             complement(4543..5673)
                     /locus_tag="VHM69_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2740810.1"
                     /translation="MRILEGAIYTVDEVSDLLGIPRPTLYRYLREYSIPHLRRAGRIS
                     IPEDSFDRIREARDLHKEGLGTETVRRQLREGSTQDTGELDRRLDNLHETLESLRGDL
                     KERPATDEVALSPTLRTILARQSLLMSAMFNLTEMVEDLLLASGKRRKPLFGDLGMGK
                     PDPLPRAKGPLEITGTVPATVGQPRAPRSTGFGTLGRRRRRGVLALLAALLLAVCLTW
                     AIPLIGAGDSVPRVKEAATEPPDKPKAVAAPGETTSTDETTSQESGAVNAAADSSEQD
                     GDLVPDVSGRSLEDAAGTLSSAGFKVAAIKTVASQEEPETIIRTEPSSGESARSGAPI
                     ILTMSGGPTGTPPGVQTASAGASATTSASASTTASASAPYTN"
     gene            5954..6535
                     /locus_tag="VHM69_10200"
     CDS             5954..6535
                     /locus_tag="VHM69_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX2740811.1"
                     /translation="MLKLSRLLLVLLFVGALVAFDNDRAEATTGLASWYGPGFEGSLT
                     ASGEVFDPYGYTAAHRTLPLGTQLTVSYAGRSVQVTVNDRGPYSGGRDLDLSQGAAEY
                     LGLTSAGVDWVDYTVTGSGGYAGDSAGYSNDSGGESYASSQPDTGGGSYVVQSGDTLS
                     GIAAQLGTSVDDLASANGIANPDLLTVGQTLYY"
     gene            6600..7628
                     /locus_tag="VHM69_10205"
     CDS             6600..7628
                     /locus_tag="VHM69_10205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2740812.1"
                     /translation="MVENGTAPTFGLWYDFRQRLPLGDYARFYAECLEEIEEGERLGF
                     TGVWLSEHHFVDDGYLPSPLVVAAAIAARTTSVTIGTNVLLLPMHHPLRVAEDAAVAD
                     LISGGRFVLGVGQGYVQHEFEVLGFKRKYRPSLFEEGIEVIRHALEVGRTGYKGKRWS
                     FDDLPFEPRPEGKLPIYIGAFAEPAIDRAARLADGFLASTGGGDFGETYRRVRAALER
                     HARADEDFPYVASGVAFVHEDAGRAREIVGPAIAYQRTRYAEWGTDRDRPRPGPIREE
                     DLPWDRYLVGTPEEVAEELIRLHQEAPYDHLCFWGRLPGVTHEQALANMRLFASEVAP
                     RVSDAVKA"
     gene            7625..>8179
                     /locus_tag="VHM69_10210"
     CDS             7625..>8179
                     /locus_tag="VHM69_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2740813.1"
                     /translation="MKVTSEILNQEQTAFLVRQRVARLATADSSGKPHAVPVCFAIAP
                     PSVYIALDEKPKDVPAIRLKRVRNIIENPDVVLVADRYAEDWSLLAFVMVRGRAELLD
                     PDTMEHSAAVRLLRGKYHQYEAMKIEENPVIAIRPEKAASWGALDEAESSEPPILDTI
                     HGRRSIRRYLPKMVPDETIEQVLEA"
BASE COUNT         1519 a         2534 c         2607 g         1519 t
ORIGIN      
        1 tcgtcggcga gcgtccctgg aaccactgcg ctgccggcct gcaaggccgc ttgttgagaa
       61 gacttccggc ccgtgagaag gccactactc tctcggaggt aaacttatga acgtcagcac
      121 cgagtacacg accatcgcac cgcacggcgg cgagctcgtc gaccgcagag ccccggagga
      181 ccagcgcgac gggctgcttg gtagagccga gggcttcgcc agggtaactc taggccccag
      241 ggctctctca gacctggaga tgatctccac aggcgtcttc tcgcccctga cgggctttat
      301 ggtcagcgag gactacgaga gcgtcgtcga gacgatgcac ctgaagaacg gtctggtctg
      361 gagcctgccg atcaccctcc ccggtggcga ggaagagtcg aacgggataa gggtggggga
      421 ggagatctcc ctgaccaacg gcgatggccg tatcctcgcg acgatgatcg tcgaggaccg
      481 ctactcctac gacaaagtac acgaagccag cgaggtcttc ggcacgacga gcgacgaaca
      541 tcccggcgtc gcggccgtct accgccagaa ggacgtcctc ctaggtggcg aggtcacgct
      601 cctcgtggac gacccgaacc cgaagccttt cccttcctac cactacgagc cgcgcgagtt
      661 gcgcaaggtc ttcgcggaga aggactggaa gcgcgtcgtc ggtttccaga ccagaaaccc
      721 cgtgcaccgc tcgcacgagt acattcagaa gagcgccctg gagatcgtag atggattact
      781 gcttaacccg ctcgtcggag agacccgctc ggaggatatc cccgccgacg tcaggatgaa
      841 atcctacgag accatactcg accgctatta tccgaaggac cgtacgatgc tcgcggtctt
      901 ccccgcggcc atgcgctacg gaggaccccg cgaggcgatc ttccacgcca tctgccgcaa
      961 gaattacggc tgcacacact tcatcgtagg ccgcgaccac gccgggccag gcaaggactc
     1021 cagcggcagg ccgttctacg gcgactacga cgcccagcac atcttcgacg atttcgagga
     1081 cggagagatc gggctgactc ctctgttctt cgagcacgcc ttcttctgcc tcgaatgcca
     1141 ggggatgggg acgaccaaga cctgccccca cagtaaggaa caccacgtct ttataagcgg
     1201 cacgaaggtg cgcgagatgc tctccaaggg cgagtacccg cctcccgagt tctcccgccc
     1261 cgaagtcatc gaagtgctca tggatgggca acgcacgtag caggattatt aagaatacgt
     1321 taaaatcgca acgtcaagtt aaccaaacgt caaggagcgc taataatgga gaatgggatg
     1381 aggaatggtc gcggcttcac gctctggttc acggggctct ccggggctgg caagacgacc
     1441 atctcggaga tcgtcgagca cgagctcagg aaccgcttcc gcaagatcga ggtattggac
     1501 ggggatatcg tgaggaccaa cctctccaag ggtcttggtt tctccaagga ggacagggac
     1561 accaacgtct tgaggatcgg gttcgtcgct gacctcctga cgcgcaacgg ggttggtgtg
     1621 atcgttagcg ccatctctcc tttcaaggag gtgcgcgacc aggtgaggcg caacatcggg
     1681 ggcgatttca tcgaggtctt cgtcgacgct ccgttggagg tttgcgccga gagggacgtc
     1741 aagggtctct acaagaaggc tttctcgggg gagataccgc agttcaccgg ggtgtccgac
     1801 ccgtacgagg cgcccgctgc tcccgagctt cacatcaaga ccgatgagga ggatgcccag
     1861 gcgagcgcga ggaaggtcat cggctacctc gaagagctgg gctacctcag gccggtcgag
     1921 gcgctcgacc gggtgtgata gacaccaccc ccgccctgtt cggcctgctg gtcggtttcc
     1981 tggtaggcct caccggggtt gggggcgggt cgctcatgac gccgtttctg gtggctgtga
     2041 tgggggttcc cgccaccacc gccgtcggga ccgacctcac cttcgcgacg ctcaccaagt
     2101 tgaccggctc cttccagcac ttccgtcaga agtcggtcaa cctggagata gccattttcc
     2161 tggggatggg aagcatcccc gctggtctgc tcggggtagg gaccctggag tggatcaagg
     2221 gcaccttcga ccccgagagg gtcgagtcga tcatgataac catcatagcc gcgaccctgg
     2281 tgcttgtcgg ggcgtccctg atctaccgcg actacttcat gcccaagagg gacgatggcc
     2341 cgaagcccgg taaatggaac ggcaagaccc gcatgagcaa caggcgcaga atgtatactg
     2401 tactgttcgg ggctctcggc ggctacctgg tcgggctcac ttcaatcggc tcgggtagcg
     2461 tgatggccgt catactgctc ctgctctatc ctatagcacc ggcggttata gtcggtacgg
     2521 acatcaccca tgccacggtc ttatctttcg tcgtaggcat agcccacatg acgcagggca
     2581 acgtgaactt caccctggcg gggaccctgc tcctcggcgg catccccggc gtcctcgtcg
     2641 gaagccggct ggcgccctgg atccctggca agccgctgaa acttatactc gccataatgc
     2701 tgatcttcgt cggcctccgc ctgctcctcg cgcaagggtg gttgtggtag gcgatcacat
     2761 gtaaggacgg ttcgcgtaag ggcggttcgc gaaccgtccc tacacgaacc atcctatcgc
     2821 cggcgctcgg accttgatgg atcacgtgcc gaaaccactg ttgtgacggc gtgaacttct
     2881 cgtaaaacca taacggtatg cgagacagga tcgcacttgt tacgggagcc ggtgccggca
     2941 taggccgtgc catagccttg cggctgtcaa aagagggtgc ggcggtcgtc gtcgccgacg
     3001 tggatgaaga gagcggtgcg gagacagttc gccggatcga ggcagacgcg ggccgcggtg
     3061 cctttgtctt ggccgacgta gcaaccgagg ccggcgcgag ggagatggtc gagttcgccg
     3121 ggcggacctt cggcggcctc gacgtactgg tcaacaacgc cggcggtgcg ccggaaccgc
     3181 acttccctga ggcggcgccc gagcattggc ttcgtaccct cgacctcaac ctgcgcggcg
     3241 tcatgctctc catccacttc ggcctcatgg cgatgcgccg tcggggaggc ggtgtgatcg
     3301 tcaacatctc atctatggct ggggtcggat accagcccta ccatgcacct gagtacgcgg
     3361 cgggcaaagc gggcgttgtg cgcctgacca cctcactggc ttacctcaag gagaacatgg
     3421 gcgttcgggt gaactgcatc tgtccagggt gggtagagac ggaggcggta cacgaggcgc
     3481 tggcgaggat gaccgagcgg gagcacgcgg cacagagcat gccgcccccg gacgtcctta
     3541 ttcaacctga tgagatcgcc gatgccgtag gcatgttcgt ccgtgacgac acgctcgcgg
     3601 ggcgggtgat gatctggccc gacggcgaac agcgacggct cgtgccaatc ggcgccatgc
     3661 cttgagaaga attcagaccc gccggagcac gactatgatg tgggtgcctg catcgagcac
     3721 gccgctctgc tcgcagaacc gtgactccca ctccagcagg gtctcccaga atacagggtc
     3781 cctgcctgcc tcttcgagca ccgcctcgtc ttgaacggag aggaagttgg aagccgaggc
     3841 tgcgactatc tcgcacgaat gacgttcgag aagggctttg agctcctccc agcgaaacat
     3901 gtgacagtgg tgcccgtgag gagcgaccac gccgtactgg tcgccggtgt caacgaccct
     3961 ctgcacctct tccatgccaa cttcgcggta gaggtcgaag atggctccaa ggaactttcg
     4021 cgtcgccccg gcgagggaca tcacgcttaa caacacgtgt ccgccgggtc tggttactcg
     4081 cagcatctcc gccagggcgt cgtcggcgcg gtcgaagacg tagctcaatg gccccccgta
     4141 gcacacgacc ccgtcgaagt tgctctcggg gaagcgcgag aggtccgtga tgtccaggga
     4201 caccctggtg acgatgcgct gctcgcagcc ggcctcagtc accttcttgc ggttgagctc
     4261 taactgaccg gacgagatgt cgcccaccgt gaccacagcg ccgagttcgg cgagttcgat
     4321 cgtgaaccgt cctgctcccg ccccggcttc caggatgtga tccccgcgcc tgacgtatcg
     4381 gtgcaggtag cgacgatgca gatggaacct tacccgtgcc ggtgcatcgg ggtcgagacg
     4441 ctcccactcg cgctcgccgt actcgtcaaa gaaccgtccg acgtgctccg gatcgtactt
     4501 ctcgccgatc gtaacccctt ctacagcaat ctgtgtttgc cctcagttcg tgtacggggc
     4561 agaggcgctg gcagtcgtac tggcacttgc gcttgtcgtc gcgcttgcgc cggcgctagc
     4621 ggtctggacc cctggagggg ttccggtagg tccgccgctc atcgtcagga tgatcggcgc
     4681 tccagatctc gcagactcgc cggacgaggg ctctgtacgg atgatcgtct cgggctcttc
     4741 ctggctcgcc acggtcttga tggctgccac cttgaacccg gcgctggaga gggtgcctgc
     4801 ggcgtcttcg aggctccggc ccgagacgtc gggtacgagg tctccgtcct gttcggagct
     4861 gtccgctgcg gcgtttacgg cgcccgattc ctggctggtg gtttcgtccg tcgaggtagt
     4921 ctcgccaggg gctgccaccg ccttcggctt gtccggaggt tcggtcgccg cctccttcac
     4981 cctgggtacg gagtcgccgg cgccgatcag gggaatcgcc cacgtcagac agaccgcgag
     5041 caggagtgcc gccagtagcg ccagtacgcc ccgacgtcgc cgtcgtccaa gggtgccgaa
     5101 accggtggac cggggcgcgc ggggttggcc cacggtagcc gggaccgtac ctgtgatctc
     5161 gagtggaccc ttcgcccgtg gcagcgggtc cggcttcccc atcccgaggt ctccaaagag
     5221 cggtttcctg cgcttcccgc tcgcaaggag caagtcctca accatctcag tcaggttgaa
     5281 catcgcggac ataaggagac tctggcgcgc caggatggtc cgcagcgtgg gggagagggc
     5341 aacttcgtcc gttgcgggac gctccttcag gtctccccgc aggctctcca gagtctcgtg
     5401 cagattatcc aaccggcggt ccagctctcc cgtatcctgg gtgcttccct cccgcagttg
     5461 tctcctgacg gtctctgtgc cgagcccctc cttgtggaga tccctcgcct ccctgatgcg
     5521 gtcgaaagag tcctcgggga tcgagatcct gcccgctctc cgcaggtgag ggatagagta
     5581 ttccctcaag taacggtaga gggtaggccg gggaatgccc agcagatcac tgacctcatc
     5641 gaccgtgtaa atagccccct ccaggattct cacacagata ttttgaaact ttttcttgcc
     5701 gatcagcatg ttaacacaaa gtccattcgg cctcgtttcg cgacaagcag cgctagggaa
     5761 agcggttttc gcggcctggc cccgataagg ctcggtacgc ctcaagccgg gccgctttat
     5821 cctgattatc acgccgtgat aacggtgaaa atggtcttga tttccctttg caaagcgcaa
     5881 atactcgacg ccgcagaaca ttgatgttga actaaaggta aatgtaaagc tgacgcagac
     5941 ggagggggat gcgttgctga aattgtctcg tttgttattg gtgttgcttt tcgtcggcgc
     6001 tcttgtggcg ttcgataatg accgtgctga ggccacgact ggactggctt cctggtacgg
     6061 accgggcttc gagggatctc ttacggcgag tggcgaggtc ttcgatcctt acggctatac
     6121 ggcggcacac aggacgctgc cgctcgggac gcagttgacg gtcagctacg ctggtcgttc
     6181 cgtacaggtt acagtcaacg accggggacc gtactcggga gggcgggatc tcgacctctc
     6241 ccagggcgcg gccgaatatc tgggcctcac gtcagccggg gtggattggg ttgactacac
     6301 cgtcacggga agtggaggct atgccgggga ttcagccgga tattcgaacg actctggagg
     6361 cgaaagctac gcttccagcc agcctgacac cgggggcggg tcctacgtgg tgcagtcggg
     6421 agatacgctg tcggggatcg cggcccagct cggtacctcc gtggacgacc tcgcttcggc
     6481 caacggcata gccaaccctg acttgctgac cgtgggacag acgctctact actgatccgg
     6541 accagggtgc cccgcgccgg ggtgtaaact aggcgcgcag gcggcatgag gagacgctct
     6601 tggtcgagaa cggaaccgcc ccgacgttcg ggctgtggta cgacttcagg cagagattgc
     6661 cgctggggga ctacgccagg ttctacgcgg agtgcctgga ggagatcgaa gagggagaga
     6721 ggctcggctt cacgggggtc tggctctccg agcaccactt cgtggacgac gggtacctgc
     6781 cctcgccgct ggtagtagcc gccgcgatag cggccaggac cacgagcgtc accataggga
     6841 cgaacgtgct gctcttgcct atgcaccatc ctctgagggt ggccgaggac gcggctgtgg
     6901 ctgacctcat ctcgggcggg cgcttcgtcc tcggagtcgg ccagggctac gtgcagcacg
     6961 agttcgaggt gctcggcttc aaaagaaaat acaggccctc gctcttcgag gaggggatcg
     7021 aggtgatccg tcacgcactc gaagtgggcc ggacaggcta caaaggcaag cgctggagct
     7081 tcgacgacct gccgtttgag ccacgccctg agggcaagct ccccatctac atcggggctt
     7141 tcgccgagcc ggccatagac cgtgccgcgc gcctcgctga cgggttcctc gcctccacgg
     7201 gcggcgggga cttcggggag acgtacagga gggtcagggc ggcgctggaa cgtcacgcac
     7261 gggctgacga agatttccct tacgtggcgt caggggtggc attcgtgcac gaggacgcgg
     7321 ggcgggcgag ggagatcgtt ggcccggcga tcgcttacca gcgcacgcgc tacgcggagt
     7381 ggggcaccga ccgcgacagg ccgcgcccag gcccgatcag ggaggaagac ctgccgtggg
     7441 acaggtacct ggtcggcacg cccgaggaag tggctgagga gctgatccgg ctgcaccagg
     7501 aggcacccta cgatcacctc tgcttctggg gccggctccc cggcgtcacc cacgagcagg
     7561 cgctggcgaa catgcgcctc ttcgcctccg aggtcgcccc gagggtcagc gacgcggtaa
     7621 aggcttgaag gtaacttcag agatactgaa ccaggagcag accgctttcc tggtacgcca
     7681 gagggtcgcc cgtctcgcga ccgcggacag ttcgggaaag ccgcatgcgg tccccgtctg
     7741 cttcgcaatt gcgccgcctt cggtctacat tgcactcgac gagaagccga aggacgtgcc
     7801 agccatccgg ctcaagcgag tcaggaacat catcgagaac cccgacgtgg tcctcgtcgc
     7861 cgaccgttac gccgaggact ggagcctgct cgctttcgtg atggtgcgtg gccgcgcgga
     7921 acttctggac cctgacacta tggagcactc ggcggccgtc aggctcctca ggggcaagta
     7981 ccaccagtac gaggcaatga agatcgagga gaaccccgtc atcgctattc gtcctgagaa
     8041 agcagcttcg tggggcgcgc tcgacgaggc ggagtcctcc gagccgccga tcctcgacac
     8101 catacacggg cgccgttcga tccggcgata tcttcccaag atggtgcctg acgagacaat
     8161 cgagcaggta ctcgaagcc
//
LOCUS       DATAIR010000416         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_303784, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000416 DATAIR010000000
VERSION     DATAIR010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303784"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="cax"
                     /locus_tag="VHM69_10215"
     CDS             <1..979
                     /gene="cax"
                     /locus_tag="VHM69_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2740814.1"
                     /translation="ELLHAPTLLIFAFTSLALVGLAWVLGQATESLGHHSGPRIGGIL
                     NATFGNAAELIITIFALSAGLTTVVKASIIGSIIGNVLLVLGASLLFGGLKNGVQSFS
                     AEIAGINASMLAIVAAALSLPTIFALTNPGSGPTTLSIEVAVVMFVLYILYLIYILRS
                     PEGGAAGGEGHAPFSRTASLVLLFAATAAVAFVSEAFVGAIQPLTEEYGISELFVGVI
                     LVPIVGNIAEHIVGVQIAYKNNMDFSMGISLGSSIQVALLVTPILVFLGPLLGTPLHL
                     TFSPLELGALGASVVVTGFVALDGKSNWLEGAMLCGVYVIAALAFYFSP"
     gene            complement(964..1854)
                     /locus_tag="VHM69_10220"
     CDS             complement(964..1854)
                     /locus_tag="VHM69_10220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740815.1"
                     /translation="MDDRDLREQNRVSWNAVVGALNSHRGDLSRFLREGGCTLFSEEM
                     RLLGDLEERSLVHLQCNSGGDSISLAKLGATVTGVDISDEAVRSARALSKKTGIPATF
                     ERADVYDWLEEASREGRRFDAVFASYGVVCWLPDLGRWTEGIAQVLEPGGCFVLVDFH
                     PAADIFDEDWNHVRDYPSGGEQLLLEEGVDDYVAGSDGGLTPAGFVEGVSGFENPEKA
                     HLFRWGLGEVVTALAGAGLRITTLQEYPYTNGERNFSGMRELTGRRMVPPEDVPAIPL
                     MYGIRAEKCLRSLKPPHGEK"
     gene            complement(1864..>2549)
                     /locus_tag="VHM69_10225"
     CDS             complement(1864..>2549)
                     /locus_tag="VHM69_10225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2740816.1"
                     /translation="SDHGRKVLRSRGVPPGRIHVLRPGFDHVPAKGDSRVERYGPVRA
                     LCVAQWIPRKGILTLIQAWKLRERPGAVLELVGETDADLDYGESVRSAIDAAPRGSIV
                     VSGSVDDEALGAAYASANLFVLPSRYEGYGIVYAEALAHGLPIVACDVGPVPDLVGRE
                     AAILVEPDDKEGLSAALDLLLGDPELRARMSGAASRRASSLPLWKSTVAGFEEVLRAA
                     ISDEARRPM"
BASE COUNT          416 a          876 c          765 g          492 t
ORIGIN      
        1 agagcttctg cacgccccga ccctcctgat cttcgccttc acgtccctgg cccttgtagg
       61 gctggcctgg gtcctcgggc aggccaccga gagcctcggc catcactcag gcccccgcat
      121 tggcggcatc ctcaacgcca ccttcggcaa cgcggcggag cttataatca ctatcttcgc
      181 cctttcggct ggcctcacca cggtggtgaa ggcttcgatc atcggctcta tcatcggcaa
      241 cgtcctgctc gtgctcgggg ctagcctcct cttcgggggc ctgaagaacg gcgttcagag
      301 cttctccgcg gagatcgcgg gtatcaacgc ctcgatgctc gccatcgtcg ccgcggcgct
      361 ctccctaccg accatattcg ctctcacgaa cccgggttct ggaccgacca ccctgagcat
      421 cgaggtagcc gtggtcatgt tcgtgctcta catcttgtac ctgatctaca tcctccgaag
      481 cccggagggc ggggccgcgg gaggcgaagg gcacgccccg ttcagcagga ctgcgtcgct
      541 ggtgctcctg ttcgctgcga ctgctgcggt ggccttcgtt tcggaggctt tcgtcggggc
      601 cattcagccc ctgacggagg agtacggaat ctccgagcta ttcgtcgggg tcatcctcgt
      661 accgatagtc ggcaacatcg ccgagcacat cgtcggcgtc cagatcgcct acaagaacaa
      721 catggacttc tcgatgggca tctctcttgg ctcctcgatc caggtcgccc tgctcgtcac
      781 ccccatcctg gtcttcctcg ggcctctgct cggcacccct ctgcacctga ccttcagccc
      841 gctggagctc ggcgccctcg gggcctccgt tgtggtaacc ggcttcgtcg ccctggacgg
      901 caagtccaac tggctagaag gcgcgatgct ctgcggcgtc tacgtcatcg ccgctctcgc
      961 cttctacttc tcgccgtgag gaggtttcag gcttcgcaag catttctccg cccgtatgcc
     1021 gtacatcagg gggatggcgg ggacgtcttc gggcgggacc atgcgtctgc cggttagctc
     1081 gcgcatcccg gagaagttcc gttccccatt cgtgtacggg tactcttgga gggtcgtgat
     1141 cctcaagccc gctccggcca gcgccgttac gacctctcca agcccccagc ggaagaggtg
     1201 tgctttctcg gggttctcga aaccgcttac cccctcgacg aaccctgctg gggtgagacc
     1261 tccgtctgag ccggccacgt agtcgtcgac tccttcttcg aggagcagct gttctccacc
     1321 ggagggatag tcgcggacgt ggttccagtc ttcgtcgaag atatccgccg caggatggaa
     1381 gtccacgagc acgaagcagc cgcctggttc gaggacctgc gcgatgcctt ccgtccacct
     1441 tccgaggtcg gggagccagc agacgacgcc atacgaggcg aagacggcat cgaaacgcct
     1501 gccctctcgc gaagcttcct ctagccagtc gtacacgtcg gcgcgctcga aggtagccgg
     1561 aatgccggtc ttcttcgaga gagcgcgggc ggagcgcacg gcttcgtcgc ttatatccac
     1621 gcccgtgacc gtcgcgccga gcttcgccag gctgatcgaa tcaccacccg agttgcactg
     1681 gaggtggact agggatctct cttcgaggtc tccgagaagc ctcatctcct cggagaacag
     1741 agtacagccg ccctcacgca ggaaccgcga gaggtctccg cgatggctgt tgagggcgcc
     1801 gaccacggca ttccacgaaa ccctgttctg ctcgcggagg tctctatcat ccacgagaga
     1861 attctacatc ggcctcctgg cctcgtccga gatcgcggcc ctcagaacct cctcgaagcc
     1921 cgcgaccgtg ctcttccaca gaggtaggct ggaggcgcgg cggctggcgg cgccggacat
     1981 cctagccctc agctcgggat ctccgagcag gagatccagg gccgcggaga gtccctcttt
     2041 atcgtcaggc tcgacgagta tcgcggcctc cctgccgacg aggtcgggta cgggaccaac
     2101 gtcgcaggct actatcggca gcccgtgggc tagcgcctcc gcgtagacga taccgtaccc
     2161 ctcgtagcgc gagggcagca cgaagaggtt ggcggatgcg taggcggcgc cgagcgcctc
     2221 gtcgtccacg gaaccggata cgacgatgga gcctcgcggt gcggcgtcga tggcggagcg
     2281 tacggactca ccgtaatcga ggtcggcgtc cgtctccccg acgagttcga gcaccgcccc
     2341 gggacgctcg cgcaacttcc acgcctggat aagcgtcagt atccccttgc gcggtatcca
     2401 ctgcgcgacg cagagggcgc gcacaggacc gtatctctcc acgcgactat cacccttcgc
     2461 cggaacatgg tcgaagcctg gccgcaagac gtggatacgt ccgggaggaa cgcccctgct
     2521 ccgaagcacc ttccgtccgt ggtcgctca
//
LOCUS       DATAIR010000417         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_441963, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000417 DATAIR010000000
VERSION     DATAIR010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441963"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1082)
                     /locus_tag="VHM69_10230"
     CDS             complement(249..1082)
                     /locus_tag="VHM69_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2740817.1"
                     /translation="MPAIQPYIIEPIWEQFRALLPERKVDHPLGCHRPRIPDCVVFEK
                     LVEVLVFGCAYWRIADGSCSATTLRRRRDEWIEAGLMDALEDMALEAYDRAIGLDLAD
                     VAVDCCITKAPCGGEKAGRSPVDRGKQSIKRSMMVDACGIPLGVVAAPANRHDSPLLA
                     PTLDALEALGLPPEPVSVHLDRAYDSSLTRRLLEDRGLVGEISEKGKPSPLGATKRWV
                     VERTNSWSNAHKKLVWCTERRGRVVNFWVTFSNVIIIVGRLVRKAWTHYRWEGRPTRR
                     P"
     gene            1226..1390
                     /locus_tag="VHM69_10235"
     CDS             1226..1390
                     /locus_tag="VHM69_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740818.1"
                     /translation="MADIYRRRRGRDTWQFCSNCSNWPTSDYVERDSKPATGELCDQC
                     EAKQRNGNCR"
     gene            1401..2495
                     /locus_tag="VHM69_10240"
     CDS             1401..2495
                     /locus_tag="VHM69_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2740819.1"
                     /translation="MKRGKDREEHSYLGVWDPAGGETREGSGLPVEEGQEEIRIVTRL
                     LRGEGKDQEISKGELKGAVEDPDLLVWVDVLYPREGAAALLRDSLELGALTVEDCMEP
                     LRMPKMDVLHGDASGGAGGAFVAAFAARLENEAGGPRLRASEVDLVAGPRYLVTLRDG
                     PVDEVERRLENLMRSPEPGASGAALAHAAFDALVDGHPPVMVRASTIAEELEEVLDPR
                     DERSSISALEALITLRRDLLAFRRLAVAQSEILRRLARVSPEVREYLSDVADNQREAV
                     DMADANRDYIEGAVEAYRVRRDERSDAGIRRLTILAGIIGPLTLLNGIYATNFNTPGT
                     QAPWSFWAFIGVQVVFLILAILYLRRRGLL"
BASE COUNT          604 a          960 c         1023 g          563 t
ORIGIN      
        1 gatcacctgg gaaccggagg ccgaccggcg catcaagcgt agccccttcc tcatgcgccg
       61 ctttatccgc aagcgcatcg aggatcgggt cgcctcccga ggccgcaccc acgtcaccga
      121 ggccgacgtg gacgagagcg ccgggatgta ccgccagtat cgtttcaagt aggcttcagg
      181 ttccacgacg agaagcaatt ggcggcactt cttgcgagga aggcccgctt agagcttgcg
      241 ccaataggtc atggtctgcg agtgggtcgg ccctcccagc ggtaatgagt ccaggctttt
      301 cggacgagcc ttcccacgat gatgatcacg ttcgagaaag taacccagaa gttcacgaca
      361 cgcccccgtc gctccgtgca ccacacgagc ttcttgtggg cgttgctcca cgagttggtc
      421 ctctccacga cccaccgttt cgtggcccca agaggggaag gcttgccttt ctcggagatc
      481 tcacccacta agccacggtc ctccaagagc cggcgggtga ggctcgagtc gtaggcgcgg
      541 tcgaggtgta cgctaaccgg ctcgggcggc aacccgagcg cctccagggc gtccagggtg
      601 ggggccagaa gcggcgagtc gtggcggttg gccggggccg ccaccacccc gagagggatg
      661 ccacaggcgt ccaccatcat cgagcgtttg atgctctgct ttcccctgtc cactggactt
      721 cttcccgctt tctcgccgcc gcacggagcc ttggtgatgc agcaatcgac ggccacgtcg
      781 gccagatcga gacctatggc ccggtcgtag gcttcgagcg ccatatcctc cagagcgtcc
      841 atcagaccgg cctcgatcca ctcgtcgcgc ctgcgacgca gcgtagtagc cgagcacgac
      901 ccatcagcga ttctccagta ggcgcagcca aagaccagta cttcaaccag cttctcgaag
      961 accacgcagt cgggtatgcg gggccggtgg cagccgagcg gatgatccac ttttctttca
     1021 ggcagcaggg cgcgaaactg ctcccagata ggctcgatga tgtatggttg gatggcgggc
     1081 acggaaatac actccttcac atcgggaagc ttcgataact ccccggatga tcgagcagcc
     1141 ggtgcccgcc ttacaagtgg tgtgctgcgt ccctattggc gcggcctcta aggagaaccg
     1201 aatcaatcca gcgaaggagg aagagatggc cgacatctat aggcgcagac gaggaaggga
     1261 tacctggcaa ttctgcagca actgctccaa ctggccgacc agcgactacg tcgagcggga
     1321 cagcaagccc gccacgggcg aactgtgcga ccagtgcgag gccaagcagc gtaacggcaa
     1381 ctgccgctga tcctgcttag atgaagcgcg ggaaggatag agaggaacac agctacctcg
     1441 gggtctggga ccccgctggc ggcgagacac gcgaggggag cgggctcccg gtcgaggagg
     1501 gccaggagga gatccgcatc gtgactaggc tactgcgagg cgagggaaag gaccaagaga
     1561 tctccaaggg agagctcaaa ggagccgtgg aggatccgga tctcctggtc tgggtagacg
     1621 ttctctatcc gcgagaaggg gccgcggccc tgttgcgtga ctccctggag ctaggggccc
     1681 tgaccgtcga ggactgcatg gagcccctga ggatgccgaa gatggatgtc ctgcacggcg
     1741 atgcttctgg cggggcaggg ggagcgttcg tggcggcgtt cgccgcccgc ctggagaacg
     1801 aagccggcgg gccgcgcctg agggcctcgg aggtggacct cgtggcgggt ccgaggtacc
     1861 tggttaccct gcgcgatggg cccgtcgatg aggtcgagag gcgactggag aacctgatgc
     1921 gttccccgga gccgggagcc tcgggcgccg cactcgcgca cgcggccttc gacgccctcg
     1981 tcgacggcca tccgccggtt atggtgcggg cctcgacgat cgccgaggag ctcgaggagg
     2041 tgctggatcc cagggacgag cggtcctcta tttcggcgct ggaggcgctc attaccctgc
     2101 ggagggactt gctggcgttc cgccgcctgg cagtcgccca gagcgagatc cttcgtcgcc
     2161 tggcgcgagt ctccccggag gtgcgcgagt atctctcgga tgtcgcagat aatcagcggg
     2221 aagccgtgga catggccgac gcgaacaggg actacataga aggggccgtg gaggcctacc
     2281 gcgtgcgtcg cgacgaacgg agcgacgccg ggatacggag gctcacgatc ctcgccggga
     2341 tcatcggtcc actcactttg ctcaacggga tctacgctac caacttcaac acacctggta
     2401 cgcaagcacc gtggagcttc tgggctttca tcggggtaca ggtcgtcttc ctgatcctgg
     2461 ccatactcta ccttcgcaga cgcggcttgc tctgatgcgc cgcggacgca catcaggacg
     2521 acgggctcga agccgtggtc ctcggagagg cgcgtgatga gatactcggc gtcatcggag
     2581 aggtcagagg tgatctggaa gtaggccgtc tgggggaagg agtcttccgg gtctgagtca
     2641 acgtgctcgt agtcggcggc catctcgtcc agtaaattcc gcatgagctc gaggtcatcc
     2701 tcgctccgga tgttgcccac acgcagtacg gtttcgccca cacctgatgg ctacccgata
     2761 tcccgctatg tagaccggcg catataagag gccgcgccaa taggcgggta gcatatgtgc
     2821 caaggggcag tcgagagact ttgcgcagcc cctcacagtt gtgatgtgcg aaaagtggcc
     2881 tgcctattgg cgcaagctct tatgacggcg accgtagaga acctatcacg atcattttac
     2941 aatgccggtt agggtaagaa ttagggtcaa ggccgatcac aaacttgagc atttcctata
     3001 tataaaggcc tgggccgtcc cgtaagccgg attctgctct atcccgccat ccatctccgg
     3061 ggaatccccg ggctcccctg aggggagctg cgcctaccag ccggacgctg cgcgcccgga
     3121 cattttggct tgctcgcacc ggtggtttac
//
LOCUS       DATAIR010000418         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_396150, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000418 DATAIR010000000
VERSION     DATAIR010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396150"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VHM69_10245"
     CDS             <1..692
                     /locus_tag="VHM69_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2088 domain-containing protein"
                     /protein_id="HEX2740820.1"
                     /translation="MVEVGDKVLESGHILFGVATVENAYDEPAIIEAIPAAKIHDREA
                     ELLAEYMEMMPGLPVSDIDVLYVDALGKNYSGTGMDTNVIGRFRILGVDEPKTPAVKY
                     VIVGDISKESHGNALGVGLADLTTRRMADQIERKAMNANVVTSTFIERAKVPMVLASD
                     EEAIETAVRCNWGVEPEDTRFVRIPNTLHLEYLYVSENMVDEALQNAEVEVVGDPEEL
                     RFDADGYFEGF"
     gene            744..1988
                     /gene="glp"
                     /locus_tag="VHM69_10250"
     CDS             744..1988
                     /gene="glp"
                     /locus_tag="VHM69_10250"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX2740821.1"
                     /translation="MQLFEKLIEYPEAERLVLESTPLLPVEETPLGEALGLALARDLR
                     ATTDSPPFDNSAVDGYALRSADAEAGRVFRVVDEAPAGRPATKSVGEGEAIKIFTGGV
                     IPDSADAVVMVENTSGWGGEFELKKAPAPGSNIREAGEDTRTGDVILRAGTEIGAPEI
                     ALAATQGYGALPVYRRPKVVILSTGTELVEPGTRDLDPGEIFDSNSYAIVAGAREAGA
                     EARRLYAASDDAETLRAAIEDALETADVVVTSGGVSVGEKDLVKSTMLDLGVEQVFWG
                     VKFKPGKPLFYGTRGETRLFGLPGNPVSAVVCFDLFVRPALMRMMGREDRRRPRIQVY
                     FEQDIVNKFGRLHAMRVVLKKTQKGWLAESVGAQGSGLVSSLTKADALALIGPASEGI
                     SAGEPIEAIVLRDEKLADADIK"
     gene            2050..>2837
                     /locus_tag="VHM69_10255"
     CDS             2050..>2837
                     /locus_tag="VHM69_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2740822.1"
                     /translation="MFESDLEDNVFRRRLMKEVAPGVPVHLSFYSPSVKVQVMYPAEN
                     LPGANGRYVGDAVTIEFVPDDAAAVERVREYVWHWEGYNAFTAVRKVNHLRRGVCPES
                     VQGILEQVSGVDRRAAAKTAEVVNPEELRELLAERDFKTLARLPGMGRKRARSVVEFY
                     ENEKGDRRFLYAANRNDRLRGKPVISELDLGGDLEEQVWKHAVRARRLGDPDPLSSGE
                     PPGHTTVRNANLLHPLPMTPSLMSRGVYYTEQIELFADTCRRVLV"
BASE COUNT          563 a          841 c          964 g          469 t
ORIGIN      
        1 tcatggtcga ggtcggggat aaggtattgg aatcgggaca catcctcttc ggcgtcgcca
       61 cggtcgaaaa cgcctacgac gaaccagcca tcatcgaagc cataccggcg gcgaagatac
      121 acgatcgcga ggcggagctg ctcgccgagt acatggagat gatgcctgga ttgcccgttt
      181 cggatataga cgtgctctac gtggacgccc tgggcaagaa ctactccggg acgggaatgg
      241 acaccaacgt gatagggcgc ttccgcatcc tcggcgtcga tgagccgaag acgccagcgg
      301 tcaaatacgt catcgttgga gacatcagca aagagtcgca cggcaacgcc ctcggggttg
      361 gtctagccga cctgacgacc cggcggatgg ctgaccagat cgaacgcaaa gccatgaacg
      421 ccaacgtcgt aacgagcacg ttcatcgagc gggcgaaggt gccgatggtc ctcgcgagcg
      481 acgaggaggc catcgagacc gccgtccgat gcaactgggg cgtcgaaccg gaggacactc
      541 gtttcgtgcg catcccgaac accttgcacc tcgagtacct ctacgtctcc gaaaatatgg
      601 tcgacgaggc gctccagaac gcagaggtcg aggtagtcgg cgacccggaa gagctgcgct
      661 tcgacgccga cggctacttc gaaggtttct gagaaagttc atccgagtcc gcagggacgc
      721 aaaagactag ttgaggtgtg agagtgcaac tcttcgagaa gctcatcgaa taccccgagg
      781 ccgagcggct cgtgctcgag agtacgccgt tgttgccggt ggaggagacg ccgctcgggg
      841 aggcactggg gctcgcgctg gcgcgggacc tcagggccac gaccgactcg ccgcccttcg
      901 acaactcggc cgtggacggg tacgcgctca ggagcgccga cgccgaggcg ggacgcgtct
      961 tcagggtcgt ggacgaggcg cctgccggac gccctgcaac gaagagcgtt ggagagggcg
     1021 aagcgatcaa gatcttcacc ggcggcgtca tacccgacag tgctgacgcc gttgtgatgg
     1081 tcgagaacac atcgggctgg ggcggggagt tcgagctgaa gaaggccccc gctcccggca
     1141 gcaacatccg cgaagcgggt gaggacacca gaacaggaga cgtcatcctg cgcgcgggga
     1201 ccgagatcgg ggctcccgag atagccctcg ccgcgacgca gggatacggc gcattgccgg
     1261 tctaccgcag gccgaaagtg gtcatcctct cgacggggac cgagctcgtg gagcctggga
     1321 cgcgggacct cgaccctggt gagatcttcg actccaactc atacgctatc gtggccggag
     1381 ccagagaggc cggcgccgag gctcgccggc tctatgccgc ctcggacgac gcggagactc
     1441 tgcgtgccgc catcgaggac gcgctagaga cggcggacgt ggtggtgact agcggcggcg
     1501 tttcggtggg ggagaaggac ctcgtcaaga gcacgatgct cgatcttggc gtggaacagg
     1561 tcttctgggg cgtcaagttc aagccaggca agcccctctt ctacgggacg cgcggggaga
     1621 cccgcctctt cggcctgcct ggcaaccccg tctccgcggt ggtctgcttc gacctcttcg
     1681 tcaggccggc gctcatgcgg atgatgggcc gcgaggacag gaggaggccg cgcatacaag
     1741 tctacttcga acaggacatc gtcaacaagt tcggcaggct gcacgccatg cgggtcgtcc
     1801 tcaagaaaac ccagaagggt tggctggccg aatccgtcgg ggctcagggc tcgggactcg
     1861 tcagctcgct caccaaggca gatgcgctcg ccctcatcgg ccctgcctcc gagggcatta
     1921 gcgcgggaga gcccatcgag gctatagtgt tgcgggacga gaagctggcg gatgctgaca
     1981 tcaaatagct tccgaaagtt gctattttgt cacaggctgg aggccgtggg gtggtagaat
     2041 ttggttccga tgttcgagtc agatctcgag gataacgtct ttcgcaggcg gctgatgaag
     2101 gaggttgcgc cgggggttcc ggtgcacctc tctttctatt cgccttcggt gaaggtacag
     2161 gttatgtacc ctgcggagaa tcttccaggg gccaacggac gctacgtggg ggacgctgtc
     2221 accatcgagt tcgtccctga tgacgctgcc gccgtcgagc gggtccgcga gtacgtctgg
     2281 cactgggagg gctacaatgc gttcacggcg gtgcgcaagg tcaaccacct gagacgcggt
     2341 gtatgtcctg agagcgtgca gggcatcctc gaacaggtca gcggcgtgga caggcgggca
     2401 gccgcgaaga cagccgaggt cgtgaacccg gaggagttgc gggagctact ggccgagagg
     2461 gatttcaaga cgctcgcgcg gctaccggga atgggcagga agcgggcccg ctccgtcgtc
     2521 gagttctacg agaacgaaaa gggcgacagg cgcttcctgt acgccgccaa ccgcaacgac
     2581 cgtctcaggg gaaagcccgt gatctccgag ctcgacctcg ggggagacct ggaggagcag
     2641 gtgtggaaac atgccgtgcg ggcgcgtcgg ctcggggacc ctgacccgct aagttcaggc
     2701 gagcctcccg gtcacacgac cgtccgaaac gcgaacctgc tgcaccctct cccgatgaca
     2761 ccttccctga tgagccgggg cgtctactat accgagcaga tcgagctctt cgccgacacg
     2821 tgcaggcgcg tcctcgt
//
LOCUS       DATAIR010000419         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_119810, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000419 DATAIR010000000
VERSION     DATAIR010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119810"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VHM69_10260"
     CDS             <1..478
                     /locus_tag="VHM69_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2740823.1"
                     /translation="NVATNGLVRTVGGADHVNEVARSLGAEDTLLYQKVSSERGAVPA
                     LDNRSTPRDMAVMLRKIAEGKAASEKSCEYMIDLMYLDELDWWLDAGLPAGVEAANKA
                     GWLYKVYDEVGIVQSGGHPYVITILSKHGTANVYEGQDLIRNLSSAVWGTQSQQGN"
     gene            complement(489..2069)
                     /gene="murJ"
                     /locus_tag="VHM69_10265"
     CDS             complement(489..2069)
                     /gene="murJ"
                     /locus_tag="VHM69_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2740824.1"
                     /translation="MTAILRSVLSISVATILSRATGYARWMAQAAALGAGYVADAYTA
                     SILLPGLIYELFMGGILYSIFIPVLVDRITKEGEEDARRLTNALFTLVLPLMVVLSIV
                     GIVFASPLVLLVTDWQSAEGLSPAEAGRTLDLAVLFFRIFILQMFFYGISTIGTGVLQ
                     AHRHFFLPTFAPVLNNLLVIGSFVAYALLVDVDSRIAFYVLAVGVTAGVAVMALALVP
                     TMLRLGYMPRPQWGHPALLPTARLAGPMVVLVAASVGFQLFGAYLATGFEALSELNYA
                     FTIFSLPYGVFVVAIATALMPELSEKHSRSDADGYRETFSFGLRTMAFVVIPSAVGMI
                     SLSQPIVGLLYERGFFDARDTEMVSSLLVVYAAGLLGYSVYFFLVRAFYSRQNTKTPA
                     ILNVAIFFLYAVLAYGLSRVLGVTGVVLALSIAYAVLAVLSLAAMRREIGRIDGGRLL
                     RSLLKALIAGAIMYAVAMTGTALLGVGSDFTERLLILVTVGGASLAVYLGVAYLLGVE
                     ELKSVIALLRRRAGKSAG"
BASE COUNT          491 a          659 c          749 g          336 t
ORIGIN      
        1 caacgtcgcc accaacggcc tcgtgcggac ggttggaggg gccgaccacg tcaacgaggt
       61 cgcccgctct ctgggtgccg aggacacgct gctctaccag aaggtgagca gcgagagggg
      121 cgccgtccct gccctcgaca acaggagcac gccacgcgac atggcggtaa tgctgcggaa
      181 gattgcggaa ggcaaggccg cgagcgagaa gagctgcgag tacatgatcg acctcatgta
      241 cctcgacgag ctcgactggt ggctcgacgc gggactgccg gcgggcgtgg aggccgcgaa
      301 caaggcaggc tggctctaca aggtctacga cgaagttggg atagtccagt ccggcggcca
      361 tccttacgtg ataacaatcc tgagcaagca cggcacggcc aacgtgtacg aggggcagga
      421 cctgatcagg aacctttcga gcgccgtctg ggggacccag agccagcaag ggaactgagg
      481 gaggtctcct agcccgcgga cttcccggcg cgccgccgca acagggcgat gacggacttc
      541 aactcctcca cccccagtag gtacgccacc ccgaggtaga cggcgagcga ggcgccgccc
      601 acagtgacca gtatgagcag acgctcagtg aaatccgaac caaccccgag tagtgccgtt
      661 cccgtcatgg cgacggcgta catgatagcc cctgctatga gggccttgag cagagagcga
      721 agcaggcgtc cgccgtctat ccgccctatc tccctgcgca tcgcggcgag gctgaggacg
      781 gcgagcacgg cataggctat ggagagcgcg agtaccacgc cggtcacgcc caagacgcgg
      841 gagagtccgt aggcgagcac agcgtataga aagaagatgg ctacgttcag gatggccggg
      901 gtcttcgtgt tctggcgcga gtagaaagcc cgcacgagga agaagtacac cgagtagccg
      961 agcagccctg ccgcgtagac caccagcaag ctggagacca tctcggtatc cctggcgtcg
     1021 aagaagccgc gctcgtagag cagtccgacg atcggttggg agagtgagat catgccaaca
     1081 gcagaaggta tgacgacgaa tgccatcgtc ctgagcccga aggagaaggt ctcacggtag
     1141 ccgtcggcgt cgcttcggga gtgtttttcg gatagttcag gcatgagagc ggtcgcgatg
     1201 gcgacaacga agacgccgta gggcagagag aagatcgtga aggcgtagtt gagctcggag
     1261 agcgcctcga accccgtggc gaggtaggcg ccgaagagct ggaagccgac ggaggctgcc
     1321 acgagcacga ccatcggtcc cgcgagcctg gctgtgggaa gcagagcagg gtggccccat
     1381 tgcgggcgcg gcatgtagcc gaggcgcaac atcgtcggga cgagcgccag ggccatcacc
     1441 gctaccccgg ccgtgactcc taccgcgagc acgtaaaagg cgatcctact gtcgacgtcg
     1501 acgagcaggg cgtaggcgac gaaggagcct atgaccagca ggttgttgag cacgggagcg
     1561 aaggtcggca ggaagaagtg tctgtgggcc tgcagtacgc cagtaccgat cgtgcttatt
     1621 ccgtagaaga acatctgcag gatgaagatc ctgaagaaca acaccgcgag gtccagcgta
     1681 cgcccggcct cggcgggaga gagcccctcg gccgattgcc agtccgtgac gagcagcacc
     1741 agcgggctgg cgaaaacgat gccaacgata gaaagcacca ccatgagcgg cagcaccagc
     1801 gtgaaaaggg cgttcgtaag ccgtctggcg tcctcctctc cctccttcgt aatgcggtcg
     1861 actaggacag ggataaagat cgagtacaga atgccgccca tgaacagctc gtagatcagg
     1921 cccggcaaga ggatggaggc cgtgtatgcg tcagccacgt accccgcgcc gagggcggcg
     1981 gcctgggcca tccatctcgc gtatccggtc gccctggaga gaatggtcgc cacagagatc
     2041 gagagcacgg acctcaggat ggctgtcata cgcgggatgt tactatagcc gggtcctctg
     2101 accctagggt acgtcttcgg ggggcatccc gaaccacttc ttgtatagcc tctcgtaagt
     2161 gccgtcggct ttgatctccg ccagcgcctc gttgaccggc tcgacgagag cgctgtcttc
     2221 ggggaaggct atgcc
//
LOCUS       DATAIR010000420         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_442136, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000420 DATAIR010000000
VERSION     DATAIR010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442136"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..2908
                     /locus_tag="VHM69_10270"
     CDS             281..2908
                     /locus_tag="VHM69_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740825.1"
                     /translation="METLIGRSTMGGFLVRRLLPAAVIVPIVLGVLRLLGERAGLYGM
                     TFGVAIMIAANVLIVSALILWSARVFDHLEDRNRLSDAALRRAEEKYRSIFDNAAEGI
                     YQTSIDGNLVTANPALARIFGYDSPDRMTSSVTDVARQLYADPDQRAEFVRRLREEER
                     VSDFEVMGRRRDGSEVWLTMSGRLLRDESGQATGVEGTVADISDQKRAASEMIRAREL
                     AEEASRAKSEFLANMSHEIRTPLNGVIGMSELLLDTDLTPEQQEYARTVHGSGESLLA
                     IINDILDFSKIEAGRLNLEKIPFDLQREVEEVVSLLAGRAHEKGLELASFVEPDVPPV
                     VHGDPFRLRQVLTNLVGNAIKFTEEGEVLVQARLDGDPNGGVDVRFEVRDTGIGMTEE
                     DQLRLFEAFSQADLSTTRRYGGTGLGLAISKQLVELMGGEIGAESELGAGSIFWFTMK
                     LEEHADGVEIGPASRANLRDLRVLLVDDNATNRKILHRQMNTWGMRDGIAENAAQAME
                     LMRDAVDSDDPYDLAILDMQMPDMDGIELARQIKSDPEFSRTRLILLTSIGIDMNQEA
                     RGAGVEALLPKPVRQSRLYDTVATVMGAGQFETLSSPEEVNRTVPGSAQEGRSPGARV
                     LLAEDNAVNQRVALKMLERLGYQVDVAADGLEAVEAASRYRYAAVLMDVQMPDMDGYE
                     ATREIRRREGESRRTPIIAMTAHAMQSDREKAFEAGMDEHITKPVTADVLKAVLERRI
                     GETAHADASTQPGNAAEEPSVDYSVLDHLRSLQTEDDPHLLKELLEMFLDDTEERLGK
                     LRAAVREEDAECIRQESHALRGSSVNMGATRVARICKELEHAGDSGNLPGASELLGTL
                     EQEFGRVRSAFQTVLLNES"
     gene            3022..>3805
                     /locus_tag="VHM69_10275"
     CDS             3022..>3805
                     /locus_tag="VHM69_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740826.1"
                     /translation="MDSSTEPASRGGEKGSENWKTLHTVALIALIVLSFVVGLIPPNQ
                     IIPGFAPPTHGLVVWIIVILIFGLCFVVIGNGTTGLWQGLLIDSRNKMSLSRLQLVLW
                     TLVVISALLTVALFNISEDPNEDPLDIIVPPQVWGLLGISTASFVTAATIKSQKKNVN
                     VSKKAKAKTTEALQKAGEDPSKLADPQGSLVAFQRPEDAGVGDFFKGDEVVSAAYLDL
                     GKVQVFFFSVIVVFTYAVEVGALLYGADAAIKSLPDLNTGIVA"
BASE COUNT          818 a         1061 c         1231 g          695 t
ORIGIN      
        1 gggcggttct acattctggc gactagagcg gtttgctgaa ggagtgaacc tgaggtgagt
       61 aaaggtttca ggctacaggc atcagcaaaa agacctgaag cctgatgcct tctccgaagg
      121 agtagctcat ccttttcgag aaccgccata aaaggtccca agtcggcgct tgctccgttt
      181 agagggcagc aaaggctggc ggccgaactc ttccgttttc ggccggcttg actagaatca
      241 ggcatcggcg gtctgtatat tgccgggaac agaggagggt ttggagactt tgatcggccg
      301 gagtaccatg ggcggcttcc tggttcggcg cctgctgccc gccgcagtca tcgtgcctat
      361 cgtcctcggt gtattgaggc tgcttggaga gcgggcaggc ctgtacggga tgacattcgg
      421 cgtcgccata atgatcgccg cgaacgtgct gatcgtctcc gccctgatcc tgtggtccgc
      481 cagggtgttc gaccacctcg aggataggaa caggctctct gacgccgcct tgcggcgggc
      541 cgaggagaag taccgtagca tcttcgacaa cgcggctgaa ggcatctacc agacgagcat
      601 agacggtaac ctggtcacgg ccaacccggc tctggctcgc attttcggct acgactcgcc
      661 cgaccgcatg acttcctcgg tgaccgacgt cgcaaggcag ctgtacgcgg accccgacca
      721 gagggcggag ttcgtccgcc gactgcgaga ggaagaaagg gtctcggact tcgaggttat
      781 gggcaggcgc agggacggga gtgaagtctg gttgaccatg agcggccgtt tgctgcggga
      841 cgagagcggc caggccactg gagtcgaggg aaccgtcgct gacatctcgg accagaagcg
      901 ggcagcatca gagatgatca gggcccgcga gctggcggag gaagcaagcc gggcgaagag
      961 cgagttcctg gccaacatgt cgcacgagat acgcaccccc ttgaacggtg tcatcgggat
     1021 gtccgagctt cttctggata cggacctcac accggagcag caagagtacg cgaggacggt
     1081 gcacggctca ggcgagagtc tgcttgccat tatcaacgac atactcgact tctcgaagat
     1141 agaggcaggg aggctaaacc tcgagaagat tcccttcgac ctgcagcgcg aggtcgagga
     1201 ggtggtctcc ctgcttgccg gacgtgccca cgagaagggc ctcgaactgg ccagcttcgt
     1261 cgagcccgac gtgccgccgg tggtccatgg agaccctttc cggctgcggc aggtgctaac
     1321 caacctggtc ggcaacgcca tcaagttcac ggaggagggg gaagttctcg tgcaggcgag
     1381 gcttgacggg gaccccaatg gcggggtgga tgtacgtttc gaggtcaggg acacaggcat
     1441 cggaatgacc gaagaggacc agctgcgcct gttcgaggcg ttctcgcagg cggatctctc
     1501 tacgacccgc cgctacggcg gtacggggct cgggcttgcc atcagcaagc aactcgtcga
     1561 gctgatgggt ggagagatag gtgccgagag cgaacttggg gcgggcagca tattctggtt
     1621 tacgatgaag ctggaagagc atgcagatgg tgtggagatc gggcccgcat cgcgcgccaa
     1681 cctcagggac ttgcgggtgc tgctcgtgga cgacaacgcg acgaacagga agatcctgca
     1741 cagacagatg aatacctggg ggatgaggga cgggatcgcc gagaacgcgg cgcaggcgat
     1801 ggagctgatg cgcgatgccg tcgacagcga cgatccttat gaccttgcca tcctcgacat
     1861 gcagatgccg gacatggacg gcatcgagct cgcccgccag atcaagtcag atcccgagtt
     1921 ctccagaacg cgccttatcc tcctgacttc tatcgggata gacatgaacc aggaggcgcg
     1981 aggggctggc gtcgaggccc tgctcccgaa gccggtcagg caatccaggc tatacgacac
     2041 cgttgccacg gtgatgggag caggccagtt cgaaacgtta agctccccgg aagaggttaa
     2101 tcgtaccgta ccgggctctg cacaagaagg acggtctccc ggagcccgcg tccttctcgc
     2161 cgaggacaac gccgtgaacc agagggtggc gctgaagatg ctggagaggc tcggttacca
     2221 ggtagacgtg gccgcggacg gcctcgaggc cgtagaggcc gcttcgagat acaggtacgc
     2281 ggccgtcctc atggacgtgc agatgccgga catggacggc tacgaggcta ccagagaaat
     2341 aaggcggcgt gaaggagagt cgcgacgtac gccgatcatc gccatgacgg cccatgcaat
     2401 gcagagcgac agggagaagg ccttcgaagc agggatggac gagcacatca ccaagccggt
     2461 tactgccgac gtgctgaaag cggtcctcga gcgccggatc ggagagaccg cacacgcgga
     2521 tgcttctaca caacccggca atgcggccga agagccttcc gtggactact cggtgttgga
     2581 tcatctgagg agcctgcaga ccgaagacga tccacacctg ctgaaggagc tgctcgagat
     2641 gttcctcgac gacacggagg agcggctcgg gaagttgaga gcggcagtcc gggaagaaga
     2701 tgcggagtgt atcaggcagg aatctcatgc cctgaggggt agctccgtta acatgggggc
     2761 gacgagggtg gcccggatct gtaaggaact cgagcacgca ggagactccg gaaacctccc
     2821 cggggcctcc gaattgctcg gaacgctgga gcaggagttc ggtcgtgtac gatcagcctt
     2881 tcagaccgta cttctcaacg aaagttagcg accgggcaag gaactcgaag tcccatctca
     2941 cgccgattca tcctagattc atcgccatct gctttcatgt attacgcagg atatagtccc
     3001 tgagacgagt gaggtaccga agtggatagc tcgacggaac ctgcgagccg tggcggagag
     3061 aaagggtccg agaactggaa gacgctgcac acggtcgcgc tcatcgccct gatcgtgctt
     3121 agcttcgtgg tcgggttgat ccctcccaac cagattattc ctggtttcgc cccgcccacg
     3181 cacggtctgg ttgtctggat catcgtgatc ttaatcttcg gcctctgctt cgtggtgata
     3241 ggcaatggaa cgacagggct gtggcaggga ctcctgatcg actcgaggaa caagatgtcg
     3301 ctctcccgcc tgcaactcgt actgtggacc ctggtggtga tatcagcctt gctcaccgtc
     3361 gcccttttca acataagcga ggacccgaat gaagatcctc tggacatcat cgtgcctccc
     3421 caggtgtggg ggcttcttgg gataagcaca gcttccttcg tgacagcggc cacgataaag
     3481 agccagaaga aaaacgtgaa cgtcagcaag aaggcgaaag ctaagactac ggaggctttg
     3541 cagaaagcgg gcgaggatcc gagcaaactg gccgaccccc agggctccct ggtcgccttc
     3601 cagcgacccg aagacgccgg cgttggtgat ttcttcaaag gggacgaggt cgtctccgcc
     3661 gcgtatctcg accttggcaa ggttcaagtt ttcttcttct ccgtgatcgt agtcttcacc
     3721 tacgccgtgg aggtgggggc cctactctac ggcgctgacg cggcaataaa gagcttgccc
     3781 gatctgaaca caggcatagt agctc
//
LOCUS       DATAIR010000421         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_304074, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000421 DATAIR010000000
VERSION     DATAIR010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304074"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2537
                     /locus_tag="VHM69_10280"
     CDS             <1..2537
                     /locus_tag="VHM69_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740827.1"
                     /translation="SFRNLRARIQRTLLTAIGIVLGVGIVFGVITLSDTMSGTFKELF
                     TRAYGSADLTVTAAGGSGGFDQKVVEKVRGYEGVRSAAPRYSLSSSLILERKNRLPEV
                     RTMRLFGVEPESAKLATGFELTGGHFPRSGTQLTLDGGSAKSAGLRIGDRVTVGTPEG
                     PRKLKLVGLLRIPGGSFGGLAFGMTPLPFAQRAFDKQGQISGIAVEATDDVSVSELKV
                     RLDKKLGEGLQVERSETRTQEISGQLQGFKLALLFFAGTSLFVGAFLVFNALSMTVLE
                     RTRELGMLRALGSTRAMIARSVIVEAALLGVLGSVLGVLFGYGMAKGLVYLFGKAFLF
                     EITTLVLTPFALVSAIVVGIAVTVVAALYPALRAGRVSPVEAMRARSGIKTTETKRSR
                     GVLRFAPVLGFLLAGAGVPWIYYLAKNLSSDLGGLVYASGITAIIAAFLGISLVIPVL
                     VRPLAALFSPLLRLLFGVEGRMAAANATRNRGRTALTASALMVGISLVVAFSALGGSL
                     LSSIRDYLDGSLGSDYVVQPTQQNSDAGFSAKLPEEVNHVPGVEKTTSIVSTFRREGQ
                     KVSLVFGVDENYPEIFRVNYASGGPDAFSKVEDGGALVGKQIAEDRNLSVGSKIKLPG
                     PQGVKKYKVEGIIKNDIIGGGMGIYMSKGTLASDFNETESEFLAIKAEPGSDRDALAR
                     RINKVLKDYPQFTLYSNAEWKAQIESDFNRQYVFFYAIMGVSVAVSAFGVVNTLSMSV
                     FERTREIGILRAVGTTRLQIGRLIIDEGIVISLIGCLVGVALGSLLGYLFVQGSGAGG
                     FEIDFYYPRLPALAALLSGLFIGVFAGLLPARSAARKDIVEAVQYE"
     gene            2605..2811
                     /locus_tag="VHM69_10285"
     CDS             2605..2811
                     /locus_tag="VHM69_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740828.1"
                     /translation="MEQTFSASVWREEGLHVAQCLEVDVASQGTTEEEALENLREALE
                     LHFEPPHATVTPLVKTIKVEVGAA"
     gene            complement(2816..2992)
                     /locus_tag="VHM69_10290"
     CDS             complement(2816..2992)
                     /locus_tag="VHM69_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740829.1"
                     /translation="MRNREVVLQLSRRLVAVAGHDSRSGTFARLPGKLDVTPFLADLC
                     EACRFQLAFDFTIG"
BASE COUNT          598 a         1010 c          993 g          627 t
ORIGIN      
        1 tgagcttccg taatctgcgc gcccgaatac agcggaccct gctcacggcc atcggcatcg
       61 tgctaggggt cggcatcgtc ttcggcgtca tcaccctctc tgacacgatg tcagggacgt
      121 tcaaggagct cttcaccagg gcctacgggt cggccgacct caccgtcacg gccgcagggg
      181 gcagcggcgg gttcgaccag aaggtggtgg agaaagtacg cggctacgaa ggagtacggt
      241 cggcggcgcc tcgctattcg ctttcctcat ccctgatcct ggagagaaag aatcgtctgc
      301 ccgaggtaag gaccatgcgc ctcttcggcg tcgagccgga gtctgcgaag ctcgcgacgg
      361 gcttcgagct caccggagga cacttcccgc gcagcggcac tcagctcacc ctcgacggcg
      421 gctcggccaa gagcgcgggg ctccggatcg gggacagggt cacagtcggc acgccggaag
      481 gtccgaggaa gttgaagctc gtcggactct tgcgcatacc cggtggttcg ttcggcggac
      541 tcgccttcgg catgacgcct ctgccattcg cgcagagagc cttcgacaag cagggccaga
      601 tctccggtat cgccgtcgag gctactgatg acgtatccgt ctccgaactc aaggtgaggc
      661 tggacaaaaa gctcggtgag ggtttgcagg tcgagcggtc ggagacgcgc acccaggaga
      721 tcagcggcca gctccagggg ttcaagctcg cgctgctctt cttcgcggga acctcgctct
      781 tcgtcggggc cttcctcgtc ttcaacgccc tctcgatgac cgtcctggag cggacgcgcg
      841 agctcgggat gctccgcgcc ctgggttcga cacgagccat gatcgcacgc tccgtaatag
      901 tggaggcggc gcttctcggc gtgctcggct cggtcctcgg ggttctcttc gggtacggga
      961 tggcgaaggg gctcgtctac ctcttcggca aggccttcct gttcgagatt actacactag
     1021 ttctgactcc cttcgccctc gtctccgcga tagtcgtcgg tatcgccgtg acggtcgtcg
     1081 ccgccctcta ccccgccctt cgcgcgggaa gagtcagccc tgtcgaggcg atgcgcgccc
     1141 gctccggcat aaaaacgacg gagacgaagc ggtcgcgcgg cgtcttgcgt ttcgcgccgg
     1201 tattggggtt cttgctcgcc ggcgccggtg tgccctggat ctactatctc gccaagaacc
     1261 tctcatccga cctcggaggg ctcgtatacg cctctgggat cacagcgatc atcgccgcct
     1321 tcctcggcat ctcattggtc atccctgtcc tcgtccgtcc gctcgcggcg ctgttctcgc
     1381 cgctactcag gcttctcttc ggggtcgagg gtaggatggc cgcggcgaac gcgacccgca
     1441 accgtggcag gacggcgctt acggcatcgg ccctcatggt cggcatctca ctcgtcgttg
     1501 cgttctcggc acttggcggc agccttctga gttcgatccg ggactacctc gacggctctc
     1561 tgggcagcga ctacgtggtg cagcccacgc agcagaactc cgacgcagga ttctccgcga
     1621 agttgcccga ggaggtcaat cacgtacctg gcgtcgagaa gacgaccagc atcgtctcga
     1681 ccttccgccg cgaaggacag aaagtgagcc tcgttttcgg ggtggatgag aactaccctg
     1741 agatcttccg cgtaaattac gcctctggag gacctgacgc cttctcgaaa gtggaggacg
     1801 gcggcgccct tgtggggaag cagatcgccg aggaccgcaa cctgagcgtg ggttccaaga
     1861 taaagctgcc aggtccgcaa ggtgtaaaga agtacaaggt cgaggggatt atcaagaacg
     1921 acatcatcgg cggcggaatg ggcatctaca tgtccaaggg cacccttgcc agcgacttca
     1981 acgagacaga gagcgagttc ctcgccatca aggccgagcc cggttcggac cgcgacgccc
     2041 tggcgcgcag gatcaataag gtcctgaagg actacccgca gttcacgctg tactcgaacg
     2101 ccgagtggaa agcgcagata gagagcgact tcaaccgcca gtacgtcttc ttctacgcca
     2161 tcatgggcgt ctccgtggcg gtctcggcct tcggggtcgt caacacgctc tccatgagcg
     2221 tcttcgagcg tacccgcgag atcggcatcc tcagggccgt agggacgacg cggctccaga
     2281 tcgggcgcct cataatagac gaggggatag ttataagcct gatcggctgc ctcgtaggcg
     2341 tggcactcgg ctctctgctc ggctacttat tcgtccaggg ctctggcgca ggaggcttcg
     2401 agatcgactt ctattacccc aggttgccag ccctcgcagc cctgctctcc ggcctcttta
     2461 tcggcgtctt cgccggcctc ttgccagcgc gctccgccgc ccgcaaggac atcgtcgagg
     2521 ccgttcagta cgagtagagt cggccctctt gaagcaagct ggttaaaatg atgccgcaca
     2581 gtggaagacg gaaggatgcc ggacatggaa cagacgttct ccgcgagcgt atggcgggag
     2641 gaaggcctgc acgtggcgca gtgcctggag gtcgatgtgg cgagccaggg tactacagag
     2701 gaggaagccc tggaaaacct acgcgaagcg ttggagttgc acttcgagcc tccccacgca
     2761 actgtgacac cgctggtgaa gacgataaag gtcgaggttg gcgccgctta agccgctacc
     2821 ctatcgtgaa gtcaaacgca agctggaagc ggcaggcttc acacaggtcg gccagaaagg
     2881 gagtcacgtc aagtttgcca ggcagtctgg cgaaggtacc agaacggcta tcgtgccccg
     2941 ccaccgcgac cagcctcctg ctcaactgca acacgacttc cctgttcctc atacctccca
     3001 ctcctttcca gcgaagggtg gccacgcact ccctgctcca gatcacacga ccatggcagt
     3061 ttgccgccac ggtatgagac ggcgcatccc ccagatgggc tatctatatc gctctttcga
     3121 tttgcgttgt aaggttcggc gatcagcatg cggtacatga acggtagcag gttcgatctt
     3181 tcaggaattc cagcgtcgcc acgagtggtg tcaacgtcta gaatggat
//
LOCUS       DATAIR010000422         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_396241, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000422 DATAIR010000000
VERSION     DATAIR010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396241"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VHM69_10295"
     CDS             complement(<1..793)
                     /locus_tag="VHM69_10295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2740830.1"
                     /translation="MQRFWETVIEPVLEAVQPKTIVEIGSDQGGNTENLLEFCRKTGS
                     TLHVIDPDPGYDVSEWQREHGERLVFHKDLSLNALPTVEDFDAVLIDGDHNWYTVFNE
                     LKTIERLCDERSRRFPLVMLHDIGWPYGRRDLYYDPDSIPEQHRKPYERKGMLPGVAS
                     LLEKDGLNQHLRNALLEGEPRSGVLTAVEDFMEETDHQLELTRLPGIHGLGILIPSQL
                     KESNAKLADFLEQLRFSSFVSRYIEGVERARLEIQILQHEERVNNR"
     gene            909..2018
                     /locus_tag="VHM69_10300"
     CDS             909..2018
                     /locus_tag="VHM69_10300"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2740831.1"
                     /translation="MPAYRSHTVIEIARGIKAFFTLAAGVTRGEVPDPYALQSTLQTQ
                     TRKLAEARRHIESQNQRIQKLQRSTSADDRQTAGRINPENIVWILGSPRTGSTWLSEI
                     LGEFSGHALWKEPFFGVVLNLRNNLAHRGYLDSKQFLLGEHYRDVWIGSMRRLFLDVG
                     HAKFPNIAPKHHLVIKEPNGSICAPMIMEAFPESKLVFLVRDSRDVVASLLDAAKKGS
                     WYGYDKFEASVADAVLRNGGPDSPENGSEDEFVERLARNYISNVGAVKEAYDRHPEGA
                     KVLVRYEDLRENPREWVFRICDSLGIEVDDEQLEQAVAKHAWENIPKENRGQGKFHRK
                     ASPGGWREDLNPEEARTVKEITGPLLREFYPESFR"
     gene            complement(2029..2931)
                     /locus_tag="VHM69_10305"
     CDS             complement(2029..2931)
                     /locus_tag="VHM69_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2740832.1"
                     /translation="MLIYGISMIRNEVDIVRTNVLYHLALGFDRLLIVDNGSTDGTTE
                     ALKQIGDRDPRVRWHRDEGPYKQREIMTVLAREAHADGADWVVQIDADEFWYAAGRSF
                     RDVLETSDAGVLRAEVENFIQRRDQRESSPAALLHMTRRAASPIWPPGRAQDLVESRK
                     IGFVEKTYPRKCISRPTAEIEIEAGNHRVSGVDGQGRRTDELFCFHAPIRSRASLEER
                     VRTATRPAEAGRKPGQGRYRRMLEGFDDEAVDREWEANSYEDGHLDVYGERHPVVFDP
                     RLRDAVSPFVSRPFWKRLLSPLQR"
     gene            complement(2941..>3637)
                     /locus_tag="VHM69_10310"
     CDS             complement(2941..>3637)
                     /locus_tag="VHM69_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2740833.1"
                     /translation="RLLVRHPHVEGGALKEIHYFDNRFYEGIEWYRRQFPPPRWMNGH
                     KTITGEATPAYLFLPYIPERVKEAAPEVRLIALLRNPVNRAYSHYHHQVRLGDESRSF
                     DEAVRAEEARLRDRHDIVPGFEYAAYSGRRLSSYLARGIYVDQLVRWTTLFDDDQLLV
                     LKSEDFFERREETVKQVLRFLGLPEQTPQEGRVRNKGDYAPMEPATRRRLEEFFEPHN
                     QRLYEYLGVDFNW"
BASE COUNT          718 a         1090 c         1101 g          728 t
ORIGIN      
        1 gtctgttgtt gactcgttcc tcatgctgga ggatctggat ctccagccgt gccctctcca
       61 cgccctcgat atagcgtgag acgaaggacg aaaagcgcag ttgttccagg aaatccgcga
      121 gcttcgcgtt gctctccttc agttgcgagg ggatgagtat cccgagcccg tgaatgcctg
      181 gcagcctcgt cagttcgagc tggtgatccg tctcctccat gaagtcctcg acggccgtga
      241 gcaccccgct ccgtggctcg ccttcgagga gggcgttgcg gaggtgctgg ttgagtccat
      301 ctttttcgag gaggctggct actccgggca gcataccctt cctttcgtag ggcttgcggt
      361 gctgctcagg gatagagtcc gggtcgtagt agaggtctcg cctgccgtag ggccagccga
      421 tgtcgtgcag catcacgagg gggaaccgtc gcgagcgctc gtcgcagagg cgttcgatcg
      481 tcttgagctc gttgaagacg gtgtaccagt tgtggtcgcc gtcgatcagg accgcgtcga
      541 agtcctcgac cgtgggcaga gcgttcaggc tgaggtcttt gtggaagacg aggcgctcgc
      601 cgtgctcgcg ttgccactcg gagacgtcgt accctggatc agggtctatg acgtgcaacg
      661 tcgagccggt cttccggcag aactcgagca ggttctccgt gttgccgccc tggtcagagc
      721 cgatctctac aatggtcttc ggttgcacgg cctccaaaac cggttcgatc accgtctccc
      781 agaaacgttg catgtattcc tacctcgttt gtgactgcat tgaagcaacg aggttaccgc
      841 ccggagcgca agaccgcaat accagggctc aagttgggtt cacaaagtcg tgatgctaat
      901 attgcctgat gcccgcgtac agaagccata cggtcatcga gattgccagg ggcatcaagg
      961 ccttctttac cctggcggcg ggtgtaaccc gcggcgaggt gcccgacccc tacgccctgc
     1021 aaagcacact ccagacacag acaaggaagc tcgccgaggc tcgacgccac atagagagtc
     1081 agaaccagcg catacagaaa ctgcaacgtt cgacatcggc agatgacagg cagacagcgg
     1141 gccgcataaa tccggagaac atcgtctgga tactcggttc tcccaggacg ggcagcacct
     1201 ggctgagcga gatcctgggg gagttcagtg gtcacgcgct atggaaagag cccttcttcg
     1261 gcgtcgttct aaacctccgc aacaacctgg cgcacagagg atacctggac agcaaacagt
     1321 tcctcctcgg tgagcactac agagacgtgt ggatcgggtc gatgaggagg ttgtttctcg
     1381 acgtgggcca tgccaagttc cccaacatcg cccccaagca tcacctcgtg atcaaggagc
     1441 ccaacggttc catttgcgcg cccatgatca tggaagcctt ccctgagagc aagctggtct
     1501 tcctcgtgcg cgacagccgc gacgtggtgg cgtccctgct ggatgcagcg aaaaagggaa
     1561 gctggtacgg atacgacaag ttcgaggctt ccgttgccga tgccgtcttg cgtaacgggg
     1621 gtcccgactc gcccgagaac ggctccgagg atgagttcgt cgagcggctg gccaggaact
     1681 acatcagcaa cgttggcgcc gtcaaggagg cttacgacag acaccctgaa ggagccaaag
     1741 tcctggtcag gtacgaagac ctgagggaga accctcggga atgggtgttc cgtatctgcg
     1801 attctcttgg catcgaagtg gacgatgagc aactcgagca ggccgtagcg aagcacgcat
     1861 gggagaacat ccctaaggaa aacagaggcc agggcaagtt ccatcgcaag gcgtccccag
     1921 gaggttggag ggaagacctc aatcccgagg aggcgcggac ggtgaaagag atcaccggcc
     1981 cgctactcag ggagttctac ccggagagct tccggtaagc tccctcagtc accgctgcaa
     2041 aggactcagg agccgcttcc agaagggacg agagacaaag ggcgacacgg cgtcccgcag
     2101 ccgcggatcg aagacgaccg ggtgacgctc cccgtagacg tccaggtggc cgtcctcgta
     2161 gctattggct tcccattcgc ggtcgacggc ctcatcatcg aagccttcga gcatacgcct
     2221 gtagcgtccc tgacccggct tgcggccggc ctcggcgggg cgggtcgccg tcctcacccg
     2281 ttcttccagc gaagctcggg agcggatagg ggcatggaaa cagaatagct cgtcggtgcg
     2341 ccgaccctgg ccgtcaaccc cggacactct gtggttgcca gcctcgatct ctatctccgc
     2401 cgtcggacgg ctgatgcact tccgaggata agtcttctcg acgaagccta tcttgcggga
     2461 ctccacgagg tcctgggcgc gtccaggcgg ccagatgggc gaggcagccc gacgggtcat
     2521 gtggagcagc gccgccggcg aggactctcg ctggtcacgc cgctggatga agttctcgac
     2581 ctccgcgcgc agcactccgg cgtcggatgt ctcgagcacg tccctgaagc tcctgccagc
     2641 ggcgtaccag aactcgtcgg catcgatctg cacgacccaa tctgctccat cagcgtgggc
     2701 ttcgcgggcg agcaccgtca tgatctcacg ctgtttgtag ggaccttcgt cgcggtgcca
     2761 gcggaccctg gggtcccgat ctcctatctg tttgagcgcc tcggtcgtac cgtcggtcga
     2821 accgttgtcc acgatcagga ggcggtcgaa gcccagagcg aggtggtaga ggacgttcgt
     2881 gcggacgatg tccacctcgt ttcggatcat ggatatgccg tagatcagca ctgtccattc
     2941 ctaccagttg aagtctacgc ccaggtactc gtacagcctc tggttgtgag gctcgaagaa
     3001 ttcttccagc cgccgcctgg tcgccggctc catcggcgcg taatcgccct tgttacggac
     3061 tctaccctcc tgcggagtct gctctggtag gccgaggaat ctcagaacct gtttcactgt
     3121 ttcctcccta cgttcgaaga agtcctcgct cttgagcacg agcagctgat cgtcgtcgaa
     3181 cagcgttgtc cagcgcacga gctgatccac gtagatacct ctggcgaggt aggaggaaag
     3241 ccggcgaccg gagtatgcgg cgtactcaaa tcctggcaca atgtcgtgcc tgtcacgcaa
     3301 ccgtgcctct tccgccctaa cagcctcatc gaaacttcga gactcatcac ccagtctcac
     3361 ttgatggtga taatgggagt acgctctgtt gaccgggttt cgaagcagtg cgatcagtcg
     3421 tacctccgga gcggcctctt tgaccctctc aggtatgtat gggaggaaca aataagcagg
     3481 cgtagcttct ccggttatgg tcttgtgccc gttcatccag cgcggcggag gaaattgtcg
     3541 acgataccac tcgatgcctt catagaagcg gttatcgaag tagtgtattt ccttcaaggc
     3601 gccaccctcc acgtgcgggt gccggaccaa gagccga
//
LOCUS       DATAIR010000423         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_304142, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000423 DATAIR010000000
VERSION     DATAIR010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304142"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VHM69_10315"
     CDS             complement(<1..837)
                     /locus_tag="VHM69_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740834.1"
                     /translation="MEQGRGVGGTSSRAAAWLAWSLVTLSVVLLVGGIAFTQMTRSTA
                     PQRPYYGPDDAVLLLATVLTFSVVGAIIASRQPRNAIGWLFCGTGLMVGINGLAGGYA
                     EYRLSGGYGPGSLAETAAWFASWSWILLVYVPTSFLLLLFPDGRLPSPRWRPVAWCAV
                     LGLIGFLAGYTLNQGPLDDFPQITNPYGVESPILEAVAIAGAILASASMVASAVSLIV
                     RMRRAGRVERQQIKWLAYGGVLVVGAVFASGVISIWSHNVSIAVVSVGLLGVPIFTGV
                     AIA"
     gene            1162..1653
                     /locus_tag="VHM69_10320"
     CDS             1162..1653
                     /locus_tag="VHM69_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2740835.1"
                     /translation="MSATDDLLRNNEAYAQSFEKSDLPLPPAKGVAVVACMDARLDVH
                     KILGLEEGDAHVIRNAGGVITDDEIRSLAISQRLLGTREILLIHHTDCGMLTFTDDEL
                     KQQIHEDVGVKPHFPMESFSDLEEDVRQSAARIQHSPYIPHKDSVRGFVYEVETGRLR
                     EVS"
     gene            1854..>3146
                     /locus_tag="VHM69_10325"
     CDS             1854..>3146
                     /locus_tag="VHM69_10325"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740836.1"
                     /translation="MTHSKPVALWAVPRSISTAFERVFVERDDFEVLHEPFSASYYYG
                     EDRRSDRFGDAEPKPEYSYENVLEDVFRPRERRVFLKDMAYHAKKLLTPEFASRFVNT
                     FIVRDPKYVLMSLYKMWPDFTLEETGYEDLYRLFRYATDAGEDVAVVDAMTFSENPAG
                     VLAAYCEHLEVPFRSGSLTWESGDVSKWDIWEGWHDDAESSTGIKPAERLDPELPEEL
                     HAAYEHCLPYYYELAAHAIPSVARRSARGRPDDDKEKILLRTETAIAPHGGDLVDRVV
                     PEEERRERSMEAAELPKVPLSPRALSDLQMISTGVFSPLEGFMLREEYEGVVEDMRLK
                     DGLAWSLPVTLSVDEERAGELAEGSEIALVDGTGEPVATMVLRELYGYDKEREARMVY
                     RTTDADHPGVAAVYRQGDVLLGGEVELLRPPDEGRFPRY"
BASE COUNT          654 a          971 c          988 g          533 t
ORIGIN      
        1 cgcgatcgcg actcccgtga agatcggcac cccgagcagg ccgacgctga cgaccgcgat
       61 gctcacgtta tggctccaga tggagatcac gccactcgcg aagacggcgc caacgacgag
      121 cacgcccccg taggcgagcc acttgatctg ctggcgctct accctccccg cccgcctcat
      181 cctgacgatg agcgagacgg cggaagccac catggaagcc gaagccagga tggcacccgc
      241 gatcgcaacc gcctccagaa tagggctctc gactccgtag gggttcgtga tctgcgggaa
      301 gtcgtcgagt ggtccctgat tcagcgtgta gcccgcgagg aagccgatga gcccgagcac
      361 agcgcaccag gccacgggtc gccatcgtgg cgagggtagc ctcccgtcgg ggaacaaaag
      421 caacaggaag ctcgtgggaa cgtataccag gagaatccac gaccacgagg cgaaccacgc
      481 cgccgtctcg gcgaggctcc ccggtccgta accgccggag aggcgatact cggcgtagcc
      541 tccagcgagg ccgttgatgc ccaccatcag gcctgtaccg cagaagagcc agcctatggc
      601 gttgcggggc tggcgggaag caatgatggc gcccaccacc gagaaggtca agaccgtggc
      661 caggagaagg acagcgtcgt ccgggccgta gtatggacgc tgtggggcgg tcgaccgggt
      721 catctgagta aacgcgatcc cgcccacgag caacaccact gatagcgtca ccagcgacca
      781 ggcaagccag gcggcggcac gactgctcgt cccaccgact cctcgaccct gttccattgt
      841 cccttctctc acgacaccac tctacaaggc gaccgtctcc cgagcgttac ggcaagcgtt
      901 acgatgtcca gtaccgagca taattgcgag aagacccttt gcacgcagtt ctgcgagtag
      961 ggcacgagcg cccaacagct agaccagtgc cacctcgacc accggctcct cccccaccgt
     1021 ggcggggaag cgcagagatc cttgtgtcct gacgcaagtg acgatgcgga aacacctttt
     1081 tgtgagggtt gccgcgtttt cgcacctgtg ctctaatctc gatccaatta tcgtattcgg
     1141 cgacacgaag gggtaggtaa gatgagcgcc accgacgatc tcttgaggaa caacgaagcg
     1201 tatgcccaga gcttcgagaa aagcgacctc ccactgcccc ccgccaaagg cgtggccgtg
     1261 gtcgcctgca tggacgcgag gctggacgtg cacaagatcc tgggtctaga agaaggcgac
     1321 gcgcacgtca tccgtaacgc cggcggcgtc ataaccgacg acgagatccg gtccctggcg
     1381 atctcgcaac gcctgctcgg tacgcgcgag atcctcctga tccaccacac cgattgcggg
     1441 atgctcacgt tcaccgacga tgagctgaaa cagcagatac atgaggatgt tggcgtcaag
     1501 ccgcatttcc ccatggaatc cttctcggac ctggaggagg acgtgcgtca gtcggccgca
     1561 cgtatccagc atagcccgta tatcccgcac aaggactccg ttcgcggctt cgtctacgag
     1621 gtcgagacgg gccggttgcg cgaggtaagc taggaaacca cggtcctcga cgatcccgct
     1681 tgacctcgaa acgccgctgg tctacagttc tccgatgctc acaaatctcg taggcaaact
     1741 aggtaattgg atgggcggta tgctcttatg acgttgtggc gtatcgcggc atcatggctg
     1801 gagagccgga aaggttgggc gctaacccgg tggagtatga gagaaaggcg caagtgacac
     1861 atagcaaacc cgttgcgttg tgggctgtac cccgctccat ttctactgct ttcgagaggg
     1921 tattcgtgga gagggacgac ttcgaggtcc tgcacgagcc gttctcggcc tcttattact
     1981 atggagagga ccgccggagc gaccgctttg gcgacgcgga gccgaagccc gagtacagtt
     2041 acgagaacgt gctcgaggac gtcttcaggc cgcgcgagcg gcgcgtcttc ctcaaggaca
     2101 tggcctacca tgccaagaag ttgttgactc cggagttcgc gtctcggttc gtcaacacct
     2161 tcatcgtcag ggacccgaag tacgtgttga tgtcactcta caagatgtgg ccagacttca
     2221 cgttagagga gaccggctac gaggacctgt acaggctctt caggtacgcg acggacgcgg
     2281 gagaagacgt tgccgtcgtt gacgcgatga ccttctctga gaatcctgcg ggtgtgctgg
     2341 ccgcgtattg cgagcacctc gaggtgcctt tcaggtccgg gtccctcaca tgggagtcag
     2401 gggacgtcag taaatgggac atctgggagg gatggcacga tgacgccgag agcagcacgg
     2461 gcataaagcc tgccgagcgc ctcgaccccg agctccctga agagttgcat gccgcctacg
     2521 agcactgcct cccctactac tacgagctgg ccgcgcatgc gatcccatca gtggcgcggc
     2581 gttccgcccg tggaaggcca gacgacgaca aggagaagat cttgctgaga accgagaccg
     2641 ccatagcccc gcacggagga gacctcgtag accgcgtggt ccccgaggaa gagcgcagag
     2701 agcgttcgat ggaggcggcg gagctaccga aggtgcccct gagcccgcgt gcactctccg
     2761 acctgcagat gatctccacc ggggtattca gccccctgga agggttcatg ctgcgcgagg
     2821 agtacgaggg cgtcgtcgag gatatgcgcc tgaaggatgg cctcgcctgg agccttcccg
     2881 tcaccctttc cgtagacgaa gagagggccg gtgagcttgc ggagggctca gaaatcgccc
     2941 ttgtggacgg tacgggggag ccggtcgcca cgatggtgct cagggagctc tacggatacg
     3001 acaaagagcg cgaggccagg atggtctaca ggacgaccga cgccgaccat cccggtgtgg
     3061 ccgctgtcta caggcagggc gacgtactgc tcggcggcga ggtggagcta ctgcgcccgc
     3121 ccgatgaggg ccgcttcccc cgctac
//
LOCUS       DATAIR010000424         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_304194, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000424 DATAIR010000000
VERSION     DATAIR010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304194"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..338)
                     /locus_tag="VHM69_10330"
     CDS             complement(177..338)
                     /locus_tag="VHM69_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740837.1"
                     /translation="MRTRAGSGPFKHEHGGTTVLTDGAEALGRAEVLAKEYPDGWVYI
                     HVEYRGDQE"
     gene            complement(443..1423)
                     /locus_tag="VHM69_10335"
     CDS             complement(443..1423)
                     /locus_tag="VHM69_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2740838.1"
                     /translation="MKALVRDKYGPPDVLELKDIDKPGFGDDEVLLKVHAASVNPADW
                     HILRGEPYIARLQLGPRKPKDRVLGCDVAGHLQAVGKNVTKFQPGDEVFGSPFMHGFG
                     AFAESVCVSEDLLASKPATLTFEQAAAVPLAASTALQGLRDHGRIEPGHRVLIIGAAG
                     GVGTFAVQIAKSFDAEVSGVCSTRNVEMVRSLGADHVIDYTQEDFTQSGQRYDLIFQL
                     AGTLSPSECRRALTSNGTLVLSSGESEGRWIGPVARIIKALVLLVFVSEKMFSFTVKP
                     NKEDLQFLKQLIEAGTLTPVIDRTYSLSEVPEAIRYLEEGHARGKVVITL"
     gene            1680..2666
                     /locus_tag="VHM69_10340"
     CDS             1680..2666
                     /locus_tag="VHM69_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2740839.1"
                     /translation="MKEIPGKVRSVLTQNVLSKVWAFLVVSRVEFMVMGLPVIAISAL
                     LAASRPNDLMGDGAVRLGLLTAVWYLAYWICSQVNCLADYELDKLYKSRLPRSIDILK
                     GPRTIWSMIAVETVVASAIVVYLAISESRVGLVVLWVVGVFLIAAYSLEPLRFKRRGF
                     LNLVTLSLILYFLPSLYIYYAMAPHLKILPLLALLGFSTQMIGLLLVNQIEDYHEDKE
                     MNVLTSTVRWGLKKASLLSLLFTAVMSVVLLVIFSMLARTPYAFVGVAVMLVAYAATL
                     RYHFKLLEAATSWEATHAQAAAQRVRQLSAQVPWWFFVAGMPLVLVAGLNLI"
     gene            complement(2881..4281)
                     /locus_tag="VHM69_10345"
     CDS             complement(2881..4281)
                     /locus_tag="VHM69_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2740840.1"
                     /translation="MEFSPMGALDIAGLQEGFRGELLRPQDPGYEDARKVWNGSIDRV
                     PALITRCAGVADVIAAVKFAKENGLLVAVRGGGHSFPGHSVCDGGLVIDLSLMKGIRV
                     DPEARTVRAQAGLLLGELDRETQAFGLAVPSGIVTHTGLSGLTLGGGINWLMRKYGLA
                     IDQLLSVDLVTAEGELVKASETENPDLFWGIRGGGGNFGIVTDFEFRLNPVGPTVLAG
                     PIFWPMEESPNVLRFYREWIAEAPDELMTIVIHRKAPPLPFVPRELHGKLVVAVVCCY
                     AGPIEEGEQVVKPLKELGSPVLDLCVPKPYLAHQAMFDLSFQHGWWYYMRACDVAQLT
                     DEVIDITVDHSLRIKSPLTAFPIWQRGGAVARVGEDETAFGGRSAGHTFNITAATETE
                     EGFDEEREWVRDFWSALEPYHTSVYVNFLMEEGEERIRQAYGARKYDRLKALKRRYDP
                     DNLFRLNQNIRPDVTA"
BASE COUNT          928 a         1474 c         1393 g         1030 t
ORIGIN      
        1 cgggtagcga gtatgtgccg cgcgaccaat gtcttctctc gctctcctct tcgataccca
       61 tttcagcgag gctagcacga ttagtgacac actgcctatc aagggttctt ggaaacatag
      121 aggcagtggt ggtctcctgg agacccatcc acgccatgct cctcttagat caccaactac
      181 tcttgatccc ctctgtactc gacgtggatg tagacccacc catcggggta ctctttggcg
      241 agcacttcgg cccgcccgag tgcctcggcg ccgtcggtga ggacggtggt cccgccgtgc
      301 tcatgtttga aggggccgct tccggcgcgc gtcctcatag gcgaggatgt agtagcggta
      361 ggtgtctttc atatctcctc ccacctgcgg agcaggtgcg ccgacttctc ctgactggta
      421 gacgagggcc ttcgggttcg cctcacagag tgatgacgac ctttcctcga gcgtgtcctt
      481 cctccagata ccggatggcc tcggggacct cgctcagcga gtacgtcctg tcgatcaccg
      541 gcgtgagagt gccggcctct atgagctgct tcaggaactg cagatcctcc ttgtttggtt
      601 tcaccgtgaa gctgaacatc ttctcgctta cgaacactaa caacacgagc gctttgataa
      661 tgcgggccac aggtccgatc caacgcccct ccgattctcc gctgctcagt accagcgtcc
      721 cattggaggt gagcgcgcgt ctgcactctg acggcgaaag agtccccgcc aactggaata
      781 tgagatcgta cctctgccca ctttgggtga agtcctcttg ggtgtagtcg ataacgtggt
      841 ctgcgccgag cgatctgacc atctccacgt tcctcgtgct gcacacacca ctcacctcag
      901 cgtcgaagga cttagcgatc tgcacggcga aggtcccaac gcctcccgca gcaccgatga
      961 tcaggaccct gtgcccaggc tcgatccgtc cgtggtcgcg cagaccttgc agggctgtcg
     1021 acgcggccag gggtacggcc gccgcctgtt cgaacgtgag ggttgcgggt ttcgatgcca
     1081 ggaggtcttc cgaaacacat acactctcag caaatgctcc aaatccgtgc atgaacgggc
     1141 tcccgaacac ctcatcaccc ggctggaact tcgtgacgtt cttgccgacg gcttggaggt
     1201 gccccgccac atcgcaaccc aggaccctgt ccttgggttt gcgtggcccg agttgcaggc
     1261 gggcgatgta cggctcgccc cgcaggatgt gccagtcggc ggggttgacg gaagccgcat
     1321 ggaccttcaa caggacttca tcgtctccga acccgggctt gtcgatgtcc ttgagttcca
     1381 ggacatcagg tgggccgtac ttgtctcgga ctagggcctt catcgagctc tcctcttttc
     1441 tccactctgc ccacgagagt agttgcaccc aatgtaacaa caccgcgagc agcggtgcat
     1501 ccccaaatat accttgtgct gggcttatct tgattccaca atacggctgt cgggcgacgg
     1561 cccggcggga aaagcgagtt gggaaggagg aagagttgtg gtctggcgcc tgtaaactag
     1621 ggctgggacc gcagcgacga ccagaatcgg gaaaaacgtg aaagtgcagg tatcaggcaa
     1681 tgaaggagat accaggcaaa gtgcgcagcg tactaacaca gaatgtattg agcaaggtct
     1741 gggccttttt agtggtcagc cgcgtcgagt tcatggtgat gggactcccg gtcatcgcta
     1801 tttctgctct cctggctgcc tcccgtccca atgacctgat gggagatggc gccgtgcgcc
     1861 tgggactgct aacggccgtg tggtacctcg catactggat ctgttcacag gtgaactgcc
     1921 ttgcagatta cgagctcgac aaactctaca agagccggct gccacggtcc atagatatcc
     1981 tcaaaggccc tagaacgatc tggtcgatga tcgcagtaga gaccgtggtc gcatccgcca
     2041 tcgtggtgta cctcgcaata tccgaatctc gtgtcgggct cgttgtgctg tgggtagtag
     2101 gcgtattcct gatagcagcc tattcgctag aaccactccg cttcaaacgg cgggggttcc
     2161 tcaacctggt tactctatcg ctcattctgt atttcttgcc ttcactgtac atctattacg
     2221 cgatggcgcc gcatttgaag atactccccc tgctcgccct gttggggttc agtactcaga
     2281 tgatagggct tctattagtc aaccagattg aagattacca cgaggacaag gagatgaacg
     2341 ttctgacttc gaccgtccgg tggggtctga agaaggcatc attgttgtcc ctcttgttca
     2401 cagccgtgat gagcgtagtc ctgctcgtca tattcagtat gctcgccagg accccatacg
     2461 ccttcgtggg tgtcgcagtg atgttggtcg cgtacgctgc gaccctacgg taccacttca
     2521 aactactcga ggccgcaact tcgtgggagg ctacgcacgc gcaggcagcc gctcaacgag
     2581 tacgacagtt gtccgctcaa gttccatggt ggttctttgt ggcagggatg ccgttggtgc
     2641 tggtggccgg gctgaacttg atctagctga gcgagctgtc acagctgtcg aatcatggtg
     2701 gcccggccgg tctcggaagc acgtcggggc actgatcttg atatccgaag gtcggagaac
     2761 cgtgaaggcg aagttcggct cactctccat tctcggctgt gcgctaaggg cggagggaag
     2821 ggccgagatc ggttagtcct ggcccttcac tctgttgctg gggtgcctca taaggggtcg
     2881 tcacgcagtg acgtcgggcc tgatgttctg gttgaggcgg aataagttgt cggggtcata
     2941 gcgacgcttc aacgccttga gccggtcgta cttcctggcc ccgtaggcct ggcggatccg
     3001 ctcctcaccc tcctccatca ggaagttcac atagacgctc gtgtggtagg gctcgagggc
     3061 cgaccagaag tccctgaccc actcccgctc ttcgtcgaat ccttcctccg tctcagtagc
     3121 ggctgtgatg ttgaatgtgt gcccggcgct tcgtccgccg aaggccgtct cgtcctcccc
     3181 aacacgggcg accgcccctc cacgctgcca gatcgggaaa gccgtcagcg gggatttgat
     3241 cctcagtgag tgatcgaccg tgatgtctat gacctcgtct gtgagctgcg cgacgtcgca
     3301 ggcgcgcatg tagtaccacc acccgtgctg gaacgagaga tcgaacatcg cctggtgggc
     3361 gaggtagggt ttgggcacgc acaagtcgag caccggcgag cctaactcct tgagcggctt
     3421 cacgacctgc tccccctcct cgatcggtcc cgcgtagcaa caaacgacgg ccacgaccag
     3481 cttgccgtgc agctccctgg gcacgaaggg aagcggtggc gccttccggt gaattacgat
     3541 cgtcatgagc tcatccggtg cctccgctat ccactcgcgg tagaaacgca ggacgtttgg
     3601 tgactcctcc ataggccaga agataggtcc ggcgagcacg gtcggaccca ccgggttgag
     3661 acggaactcg aagtcggtga cgatgccgaa gttcccgcct cccccccgta ttccccagaa
     3721 gaggtcgggg ttctccgtct cgctcgcctt cacgagttcg ccctcggcgg tgaccagatc
     3781 caccgagaga agctggtcga tggcaagtcc gtactttcgc atcagccagt tgatcccgcc
     3841 tcctagagtg agtccagaaa gcccggtgtg cgttacgata ccggacggga ccgccagccc
     3901 gaacgcctgt gtctcccgat caagctctcc gaggagcaat ccagcctggg ccctcaccgt
     3961 gcgcgcttcc ggatcgacgc ggatgccctt catcagcgag aggtcgatga cgaggccgcc
     4021 gtcacaaacc gagtggccgg ggaagctgtg ccccccgcca cgcacggcga ccagaaggcc
     4081 gttttccttc gcgaacttca cggccgcgat gacgtcggcc acgccggcgc accgcgtgat
     4141 gagggccggg actcgatcga tagacccgtt ccacaccttg cgcgcatcct cgtagccggg
     4201 gtcttgaggg cggagcagct cgcccctgaa gccctcctgc aaccccgcga tgtccagtgc
     4261 tcccataggt gaaaattcca tagcgtccgc catagttacc aatcctcctt gcccactgtt
     4321 aagggactcc tgcgggctat aaccctttcc acaaactcac ataagaccgg gccagcgatg
     4381 cctgcggcgg cgattaccgg gtggcggtat tggccatggg cggtttctat ccccgcaggt
     4441 taggctggtt gctcatctct ttctactccc ttccccacgc tcctaccggg acatttgcgc
     4501 ccatggtagc aacattgcga ggagacacgt atccctcaaa tgggtgattt ctggtgcggt
     4561 ggtagcgtat tgcgacaacg ggactgcgct tcaacagcaa gcgtgcgcca gcctaaacaa
     4621 atatcggaga aaagcccaca gaggaagctc gcccagaaag cctattgccc cggtccgatc
     4681 ggcacaggag ctgccatatg gtatcgcaca tccggaaggt cttatgaagg ctcatttccc
     4741 gtagtcagat gagcacatag agcgcaccac caccgaacac aaagagcgcg agggccaggg
     4801 tgaagctgaa catagtgctg cggct
//
LOCUS       DATAIR010000425         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_304197, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000425 DATAIR010000000
VERSION     DATAIR010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304197"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VHM69_10350"
     CDS             complement(<1..798)
                     /locus_tag="VHM69_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2740841.1"
                     /translation="METEGRRLDVLTIGEMVVDFISAEKTDTLSNASTFRRYLGGSPA
                     NIAVYISKLGGTSAVISKTGIGAFGKFLKSELQRHGVTTDYLLMDHRTHTTVIFVSST
                     AATPDFEEFRSGDYLLRPEEVSEEAISLTREDARRHFDFNMDEETLEETALREFHDLG
                     ARIVILTRSGGVVTVSENGNVERLGPLPKVHVENVTGARDAFWSALLIAHLDGKDWPE
                     AVRFAHEVASLKLKVEGHVSPNWQHGRGNREGHHYDRLPLAQGGRSQV"
     gene            984..1673
                     /locus_tag="VHM69_10355"
     CDS             984..1673
                     /locus_tag="VHM69_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX2740842.1"
                     /translation="MRRTYLVLLFGLALAMLCVGPAWGQASSFSDDFGSFDTVRWSKG
                     VHTLGRSYLDPANVDVDGQNLRIKLPARSYEGGEILTNELHGYGSYGARMKLPNAPGS
                     ITGFFLYKAPDYESEIDIEIYNDSTRRIMFTTYAGGAQTHTETVPLPFDPTAGFHEYR
                     FDYLYEPGTGQASVTFYVDGSKMSSWNTGIPQTSMHLMLNSWFPNWLESRKPKKTTYT
                     YVDRISFSVQP"
     gene            complement(1708..2166)
                     /locus_tag="VHM69_10360"
     CDS             complement(1708..2166)
                     /locus_tag="VHM69_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2740843.1"
                     /translation="MNRESVDEGTSLRLVDEFAAFGPLYMKWARSRLQDRGMTYARMR
                     LLGALHCGGPQIMTSISDELGVTRRNVTALVDALEEEGLVRRKPHPTDRRATVIEMTV
                     QGERTMDRIYDEHRASVAELFAALGEEDREELSRMLGVLREALRREGICG"
     gene            2318..>2785
                     /locus_tag="VHM69_10365"
     CDS             2318..>2785
                     /locus_tag="VHM69_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2740844.1"
                     /translation="MGTGARTLDRRGIGGIAGTGVLLVALGASVWGTDTVLRAPLGAA
                     FEPVLLVLGEHLVLALYAVPVVLLGWREFRSLDLPRWGALIVVGWGGSAVATILFTTG
                     LFKAFEAGAGSVNTVLLLQQTQPLFAIAAAAVVLGERLTVLYAPIFAVAAIGAY"
BASE COUNT          500 a          878 c          830 g          577 t
ORIGIN      
        1 aacctgtgat cgaccgcctt gcgctagagg caaccggtcg tagtggtggc cctcgcgatt
       61 gcctcgacca tgctgccagt tcggtgatac gtgcccctcg actttcagct tcaggctcgc
      121 gacctcgtgg gcgaagcgga cggcctcggg ccagtccttg ccgtcgaggt gggcgatgag
      181 gagtgccgac cagaaggcgt cgcgggctcc cgtgacgttt tcgacatgca ctttcggcag
      241 cggacccaag cgctccacgt taccgttctc cgagacggtg acgacaccgc cagagcgtgt
      301 gaggatgacg atcctggcgc cgaggtcgtg gaactctctg agggccgttt cctctagcgt
      361 ctcctcatcc atgttgaagt cgaagtgacg tcgggcgtct tctcttgtga gagagattgc
      421 ctcctcggaa acctcctcgg gcctgagcag gtagtcgccg ctgcggaact cctcgaagtc
      481 gggggtggcc gcggtggagg agacgaagat gaccgtggtg tgcgtgcggt ggtccatcag
      541 caggtagtcc gtggtgacgc cgtggcgctg gagctcgctc ttgaggaact tcccgaaggc
      601 tcctatgccg gtcttggaga tcaccgccga tgtgcccccg agctttgaga tgtagaccgc
      661 gatgttggcg ggcgaccctc caagataccg cctgaaggtc gaggcattgc tcagggtgtc
      721 ggtcttctcg gccgagatga agtcaactac catctccccg atggtgagga cgtccaggcg
      781 tcgcccctct gtttccatct ccgcctgccg ctcctctctg ccgggtcaat gctccgggca
      841 agtattccaa aatcctgtgg cgtgcgccac gcgagaactc gtccgcgggc ggaaaaatcc
      901 tccaaatggg ggatacgaca caccggtttc ggtggtacgt tgcgaaccca cctgcctcat
      961 gtggaggctt gaaaggagcg cgagtgcggc ggacttatct ggttctactc ttcggtttgg
     1021 cgctggcgat gctatgcgta ggccccgcct ggggacaggc gagcagcttc tcggacgact
     1081 tcggctcctt cgacacggta cgctggtcga agggcgtcca caccctgggt cgcagctacc
     1141 tcgacccggc caacgtggac gtagacggcc agaacctgcg tatcaaactg ccagcccgca
     1201 gctacgaagg gggagagatc ctcactaacg aactgcacgg ctatggctcc tacggcgccc
     1261 gcatgaagct ccccaacgcg cccggttcca taaccggctt cttcctctac aaagctcccg
     1321 attacgagag cgagatcgac atagagatct acaacgactc gacacgcagg atcatgttca
     1381 caacatacgc tggcggcgcc cagacccaca ccgagaccgt gccgctgccc ttcgacccca
     1441 ccgcaggctt ccacgaatac cgcttcgact acctatacga accgggcacc ggtcaggctt
     1501 ccgtaacgtt ctacgttgac ggttcgaaga tgtcctcctg gaacacaggg ataccgcaga
     1561 cctccatgca cctgatgcta aatagctggt tcccgaactg gcttgagagc aggaagccga
     1621 agaagaccac atacacctac gtggaccgga tcagcttctc cgtccaaccg tgaggcgcta
     1681 atgaaaagcg gccggaggga agttcttcta gccgcagata ccttctcgcc gcaacgcctc
     1741 gcgaagtacg ccgagcatcc gtgaaagctc ctccctgtct tcttccccca acgccgcgaa
     1801 aagctcagcc acagatgcgc ggtgctcgtc gtagatcctg tccatcgtcc gttcaccctg
     1861 aacggtcatc tcgataacgg ttgcccgccg gtccgtagga tgcggcttcc gccttaccag
     1921 cccttcttcc tccaacgcgt cgaccagcgc cgtgacgttg cgccgcgtga caccgagctc
     1981 gtcgctgatg ctcgtcatga tctggggtcc cccacagtgc agcgccccaa gcaaccgcat
     2041 cctggcatac gtcatcccac ggtcctgtaa ccgcgacctg gcccacttca tgtacaacgg
     2101 cccgaaagcg gcgaactcgt ccacgagccg cagcgacgtc ccttcgtcta ccgattcccg
     2161 attcatcaca ctcataacca gcctcgcacg agatgctcct cgtaactctt cttgcgcatc
     2221 ttaaccgttg acgtatattg ttgtcaatgt taatgtgtat atatacatga taatcaaccg
     2281 ctgtctatta cgggttgaca gaaggagcgg gtagctggtg ggcactggag ccaggacgct
     2341 ggaccggcgg ggaatcggcg gcatcgccgg cacgggcgta ttgctcgttg cgctcggggc
     2401 ttccgtgtgg gggacggaca ctgtgttgcg tgccccactg ggcgctgctt tcgagcctgt
     2461 gctgctcgtt ctgggcgagc atctggtcct ggcgctgtac gcggtgccgg ttgtgttgct
     2521 tgggtggcgt gagtttcgga gcctggacct gccgcggtgg ggcgctctca tcgtcgtcgg
     2581 gtggggtggg tccgcggtcg ccaccatcct gtttaccacg ggtctattca aagcgttcga
     2641 ggctggtgct ggttctgtga acaccgtgtt gctactgcaa cagacgcagc cactattcgc
     2701 catcgcggcg gcggcggtgg tcttgggaga gcggctgacc gtgttgtacg ccccgatctt
     2761 cgccgtcgcc gccatcgggg cttat
//
LOCUS       DATAIR010000426         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_196995, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000426 DATAIR010000000
VERSION     DATAIR010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196995"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="dnaX"
                     /locus_tag="VHM69_10370"
     CDS             complement(<1..1500)
                     /gene="dnaX"
                     /locus_tag="VHM69_10370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX2740845.1"
                     /translation="MKHTTLYRKWRPRRFGGIVGQEPVVRTLRRAIETDRVSHAYLFS
                     GPRGTGKTSTAKVLAMGLNCEKGPTPEPDGTCESCRAIMNNSSMDVLEMDAASNRGID
                     EIRDLRDKVNLAPASGRMRVYIIDEVHMLTAEAFNALLKMLEEPPEHVVFVLATTEKH
                     KVLPTIISRCQSFDFRRPSIETLAQKLKEIAEAEDIEVEPEALTVIAREGRGSFRDAE
                     GLLDQLSSFSEGGITASMVRELLGGVGPEALVETTYALYERRAADALRIVDRLSNEGK
                     DLGQFVTELLAHLRNLMLLPHAPEVALAGVGADERAPLAEQADAIPTAEVVRLVEALG
                     DSLGRIRRGGDPKLELELAFLKLARDYTEPDIEALLSRLETLEKAMENGGVTNPLRHS
                     APQNAAEDLREEPRSVAKEPGHEPVREEPPADPAPVPEEDAGARRGGGVDLASEWPGI
                     MDELKRRRQALTAAVYGEARVESLDGNVLRLVFPEEQSFHVGMARDPGHV"
     gene            complement(1751..2017)
                     /gene="ffs"
                     /locus_tag="VHM69_10375"
     ncRNA           complement(1751..2017)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHM69_10375"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          340 a          706 c          647 g          383 t
ORIGIN      
        1 gacgtggccc gggtccctcg ccatcccgac gtggaagctc tgctcctcgg ggaagacgag
       61 ccgcagcaca ttcccgtcga ggctctcgac cctcgcctcg ccgtagaccg ccgccgtaag
      121 cgcctgccgg cggcgcttga gctcgtccat gatcccaggc cactcggagg caagatccac
      181 accgcctccc cgcctggcgc ccgcgtcctc ctccgggacc ggagccgggt cggccggtgg
      241 ctcctcccgc acgggttcgt gcccgggctc ttttgctacg gaccgcggct cctcacgcaa
      301 gtcctctgca gcgttctgcg gagctgaatg tcgcagagga ttcgtgacgc ccccgttctc
      361 catggccttc tccagcgtct ccaggcggct gaggagggct tcgatatccg gttccgtgta
      421 gtcgcgggcg agtttgagga aggcaagttc cagctccagc ttcgggtctc ctccgcgtct
      481 gatgcgccca agggagtcgc ccagtgcttc gacgagccgc acgacctcgg ccgtggggat
      541 ggcgtccgcc tgctctgcga gcggggcgcg ctcgtcggcc ccaacccctg caagcgcaac
      601 ctccggggcg tgcgggagga gcatcaggtt ccgcaggtgt gcgaggagct cggtgacgaa
      661 ctggcccagg tccttcccct cgttggagag gcggtcaact atgcgcaggg cgtccgccgc
      721 cctgcgctcg tatagggcgt aggtagtctc gaccagcgcc tccggtccga cgccgccaag
      781 cagttcgcgc accatggagg cagtgatccc accctccgag aaggatgaga gctggtccag
      841 aagaccctcg gcgtccctga aagagcccct tccctcgcgc gcgatgacgg tcagcgcctc
      901 gggctcgacc tcgatgtcct cggcctccgc gatctccttc agtttctgcg cgagcgtctc
      961 gatgctcggg cgacggaagt cgaagctctg gcagcggctg ataatcgtcg gcaggacctt
     1021 gtgcttctcg gtcgttgcga ggacgaatac gacgtgctcg ggaggctctt cgagcatctt
     1081 gaggagggcg ttgaaggcct cggccgtcag catgtggacc tcgtcgatga tgtagaccct
     1141 catccggccc gaggccggcg cgaggttaac cttgtctctg aggtccctga tctcatctat
     1201 cccccggttg gaggcggcgt ccatctcgag cacgtccatc gaggagttgt tcatgattgc
     1261 cctacaactc tcgcacgtcc cgtcaggctc gggcgtcggc cccttctcgc agttgaggcc
     1321 catcgcgagc accttcgccg tgctcgtctt ccccgtccct ctcgggccgg agaaaaggta
     1381 agcgtgcgag acgcggtcgg tctcgatggc ccgccgcaaa gtccgcacga ccggctcctg
     1441 cccaacgatg ccgccgaagc gccgcggacg ccacttgcgg tagagcgtcg tgtgcttcat
     1501 gtgaagagac cgaggttgga aaaactcacg ctgctagtat aacggacgct ccaccgacaa
     1561 ccatcgcttt ttacaggctt caggtatcag gttacaggct tcaggtcgcg gtagagttgc
     1621 tcaaactgca agccgtcgtc cgtcacgaag cgtgggcgga tggcggtacg cggagggttt
     1681 cgccacagcg agagaacctg acgtctgaaa ggcgcgaagc gccgtgctga gacctgatac
     1741 ctattaaaaa ccgcgcaccc cgccttcgac gcgaccacat acggcgaact tccggccgcc
     1801 gactccggtc tggctacccc gtagcacatg aggttacccg cttagtgctg ctaccttccg
     1861 gtcctgacac ggttcacggg gccccattgc ccgggaccag gccatcaacg ccggccgcac
     1921 cggtttcaac cgaacccggt tcgcgcctca ggcggggaat taggccctgc taggcgatcg
     1981 caggtacagg gcatcgccat ctccccgctt agcgcggctt gccgattcta ccagaacgcc
     2041 acgaggtgtc agcgtgtatc acctgaggaa gcggaa
//
LOCUS       DATAIR010000427         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_120171, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000427 DATAIR010000000
VERSION     DATAIR010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120171"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VHM69_10380"
     CDS             complement(<1..1095)
                     /locus_tag="VHM69_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX2740846.1"
                     /translation="MPRTTEILVQKNVPATMRDGTTLMSNVYRPAEGGGYPVLLTRLP
                     YGKDLPLGSSVLDPLKAAEAGYIVVVQDVRGRYRSEGKFVPFVKEYEDGYDTVEWAAG
                     LPGSNGAVGMYGLSYFGKTQWQAAVMGPPSLKSMVPVVTWGNHLNGVQMRGGVQELGL
                     MHYWAQTALALDLMFRKYAGDPEQISEKLPELVGTIDTLLAGGGYDSLPLADLPTPDD
                     LTPFVRGGFQRGVDDEGWDYLNIDGKYENVDVPTFHIGGWYDCFLGETLRQYEAMKER
                     SREAGMRQPRLLVGPWTHGDFGSTLGELDFGIGSSGLFMNYRGDLTDAHLRWFDATLK
                     GDESALDSTPPVQVFVMGENRWRRYEEWPVP"
     gene            complement(1243..>1542)
                     /locus_tag="VHM69_10385"
     CDS             complement(1243..>1542)
                     /locus_tag="VHM69_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740847.1"
                     /translation="IVLSALRLARIGDDEVFFAVFFGTFIMLLVFNLVDLLILDWLVF
                     NTLRPGFVVLPGTEGARGYSDYGFHFRAFLKGVVGALVVSFIVAGATSGISAVAA"
BASE COUNT          299 a          475 c          497 g          271 t
ORIGIN      
        1 agggacgggc cactcctcgt agcggcgcca gcggttctct cccatgacga agacctgaac
       61 gggaggggtg ctatcgagcg cgctctcgtc gcccttcagg gtcgcgtcga accagcgtag
      121 gtgggcgtcc gtgaggtcgc ccctgtagtt catgaagagg cctgagctgc cgataccgaa
      181 gtcgagttca cccagggtgc tgccgaagtc cccgtgcgtc caggggccga ccagcaggcg
      241 aggctggcgc ataccggcct cgcgcgaacg ctctttcatc gcttcgtatt ggcgcaacgt
      301 ctccccgaga aagcagtcgt accagccgcc gatgtggaag gtgggcacgt ctacgttttc
      361 gtacttgccg tcgatattga ggtagtccca tccctcgtcg tcgacgccac gctggaagcc
      421 gccccgtacg aagggggtca ggtcatcagg ggtcggcagg tcggcgagcg gaagagaatc
      481 gtagccgccg ccggccagga gggtgtcgat ggtcccgacc agctcaggga gcttctctga
      541 gatctgctcc ggatcccccg catacttgcg gaacatgagg tcgagggcga gcgcggtctg
      601 cgcccagtag tgcatcaggc caagctcctg gacgccgccg cgcatctgga cgccgttgag
      661 gtggttgccc cacgtgacga cggggaccat gctcttcagc gagggcggcc ccatgacggc
      721 ggcttgccac tgggtcttgc cgaagtacga gaggccgtac atgccgaccg ccccgttcga
      781 gccagggagc cccgcggccc actcgacggt gtcgtagccg tcctcgtact ccttgacgaa
      841 ggggacgaac ttaccttccg aacggtagcg tccgcgaacg tcctgcacta cgacgatgta
      901 gcctgcctcg gccgccttta acggatccag gaccgagctg cccagcggga ggtctttccc
      961 gtatgggagg cgtgtcagga ggacggggta cccgccgcct tcggccggac ggtagacgtt
     1021 ggacatgagc gtcgtgccgt cacgcatcgt ggcggggacg ttcttctgaa ccaggatctc
     1081 tgttgtgcgc ggcatctgcc tcctccgaag ctcacttcgg ggtaatttac caggaggata
     1141 gtgcaaacgg ggcagggctt cgctgtgtgg ggagcgaaat cgggacgcct cctttcgcgg
     1201 acgacgctct cggtgacgtc tcggttctac gaccagagga tctcaggccg cgacggcgct
     1261 gatccctgaa gtcgctccgg cgactatgaa gctcacgaca agagccccaa ccacgccctt
     1321 caagaacgcc ctgaaatgga aaccgtaatc actgtagccc ctagcgcctt cggtgccggg
     1381 caagacgacg aaccctggtc tgagcgtgtt gaaaacaagc cagtcgagga tcaacaagtc
     1441 aaccaggttg aagacgagca acataatgaa ggtgccgaag aaaacagcga agaaaacctc
     1501 atcatcgccg atccgtgcga gtctaagggc cgacaggacg at
//
LOCUS       DATAIR010000428         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_243388, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000428 DATAIR010000000
VERSION     DATAIR010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243388"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..523)
                     /locus_tag="VHM69_10390"
     CDS             complement(227..523)
                     /locus_tag="VHM69_10390"
                     /EC_number="4.2.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate lyase"
                     /protein_id="HEX2740848.1"
                     /translation="MKQPEDCESIEDVRRAIDALDREVISLIDRRARYVEAAASFKTG
                     ERSVRAPERQQAMLADRRLWAEEAGLDPDVIEEIYRTLVSYFVDREMQHWRDVT"
     gene            complement(520..1500)
                     /locus_tag="VHM69_10395"
     CDS             complement(520..1500)
                     /locus_tag="VHM69_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740849.1"
                     /translation="MEEIRTQLHDALSPGYTALSESAALVEFPQPTILRLTGKDPVGM
                     LAAVLTNEIPPGERIGVYAALLNPKGRVQTDLRVSKNGDEILVDTGPEGAAAAKEILG
                     RYAPFSRVKMEDLSSGDDAWGMLGLYGPQAKELIGAPDLTEHETATITVGESQVLVAG
                     VARPVGGYDLICPSSTLDAIRDHLLETGATLANQEDYETARISTGIPRFGVDVTPDNF
                     PGESDNFLQRTVSFGKGCYPGQETVARMHYRGSPNKKLYRFELEPGSTEPPEAGDEIF
                     QGEDTVGFLTSIAPLQVDGKSYALGYLTRKADPDSPMSAEKATVLSTSPA"
     gene            1546..>1735
                     /locus_tag="VHM69_10400"
     CDS             1546..>1735
                     /locus_tag="VHM69_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2740850.1"
                     /translation="MIKVVVLDVDGTLMDTNYQHTEAWARALADVGRPVPRAEVHRGI
                     GKGSEILISDFVDDRDAAE"
BASE COUNT          317 a          497 c          542 g          379 t
ORIGIN      
        1 cccctaacaa cgtaaagaga tcacgagcaa gaacttctct tgaaaaccac gaaatcccgc
       61 tatttttgag ggatttcgtg atccagatca cgccgcgcac cagcctctcg ggaaccattg
      121 tcagcacgcc ccaccatagg ccgcttgtaa cacctgagtg ggcgaggaac acccgattat
      181 tgggaatcat tcccaataaa tcaggattgg ttcgcttgaa tgacgtctat gtgacgtctc
      241 tccagtgctg catttcgcgg tccacgaagt acgagacgag tgtgcggtag atctcctcga
      301 tcacgtcggg gtcgagcccg gcttcttcgg cccaaaggcg gcgatctgcg agcatcgctt
      361 gctgacgttc gggtgcgcgt acgcttcgct ctcccgtttt gaagctggct gccgcctcga
      421 cgtatctggc gcggcggtcg atcagggaga tcacctcccg atccagagcg tctatcgccc
      481 gacgaacatc ttcgatgctc tcgcaatcct cgggttgctt catgctggtg acgtggacaa
      541 gactgtggct ttttccgcgc tcatgggtga gtcaggatcc gcttttcggg tgaggtagcc
      601 tagcgcataa gacttcccgt ctacctggag tggggcgatg gatgtaagaa agccaacggt
      661 gtcttcgccc tggaagatct cgtctcccgc ctctggcggc tccgtggagc cgggctcaag
      721 ctcgaagcgg tagagcttct tgttcgggct tccgcggtag tgcatccgcg ccacggtctc
      781 ctgtcccgga tagcagccct tcccgaagct gaccgtccgt tgcaggaagt tgtcggactc
      841 gccggggaag ttatccgggg tgacgtccac tccaaagcgg ggaatgccgg tcgagatgcg
      901 cgctgtctcg taatcttcct ggttggcgag ggtagctccc gtttcgagca gatggtcgcg
      961 gatagcgtcg agggtggagg aagggcagat caggtcgtaa ccacccacag gtcgcgcaac
     1021 tcccgcgacg agtacctgac tctcgccaac cgtaatcgtg gctgtttcgt gctcggtgag
     1081 gtcaggagct ccgatgagct ccttcgcctg cggcccgtag aggccgagca tgccccacgc
     1141 gtcatcgcct gacgagagat cctccatctt cacgcgggaa aacggagcat agcggccaag
     1201 tatctccttc gccgcagccg ctccttctgg accggtgtcc acgaggattt catccccgtt
     1261 cttcgagact cgaaggtcgg tctgtacgcg gcccttcggg ttcaggagcg cggcgtagac
     1321 gccgatgcgt tcgccgggtg ggatctcatt cgtcaggacg gcggcgagca tccccacagg
     1381 gtctttgcct gttagccgga gaattgtcgg ctggggaaac tcgaccagcg cggcgctttc
     1441 ggagagcgcg gtgtatccgg gggatagggc atcgtggagt tgggttctga tttcttccat
     1501 aggggtattt taggacatgg atcccaccta cgaggaggtc gtattttgat aaaggtcgtt
     1561 gtgctggacg ttgacggcac tttaatggac accaactacc agcatacgga ggcctgggcg
     1621 cgcgccctgg cggatgtggg ccgcccggtg ccgagggccg aagtgcatcg cggcatcggc
     1681 aaggggtcgg agatccttat ctccgatttc gtcgatgata gggacgcggc ggaga
//
LOCUS       DATAIR010000429         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_120379, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000429 DATAIR010000000
VERSION     DATAIR010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120379"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VHM69_10405"
     CDS             <1..495
                     /locus_tag="VHM69_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740851.1"
                     /translation="AGNGDDGVSIVEGRGNSVLSNFIFSNTGLGMNLFPDGVTANDTD
                     DQDTGANNLQNFPVITSAIKSSTSPFFTIIRGTLNSNPNQTYTIQCFVAVPDPSGHGE
                     GQIPVGADTIQTDAGGDADETDSFTCASPVPQLGQAVTVTATNSTTGDTSEFSLNVGV
                     TPGP"
     gene            complement(644..1300)
                     /locus_tag="VHM69_10410"
     CDS             complement(644..1300)
                     /locus_tag="VHM69_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740852.1"
                     /translation="MPQVPDEAFRRTDEAPDEVFYRTPRLVTHIDDAAIAAVTQLYRE
                     FLPEGGEILDLMSSWVSHLPPEVEYRRVMGLGMNEVELRRNERLDSYVVQNLNTDPRL
                     PFGDGEFEGAGICVSIDYLTRPVEVLRDVGRVLKVGAPVVITFSNRCFPTKAIAIWHQ
                     LDDRGHMQLVERYLRDAGNFESVRGLDRSPRRLFSDPLYAVVGESTGPYAGGRETSRT
                     "
     gene            1409..1882
                     /locus_tag="VHM69_10415"
     CDS             1409..1882
                     /locus_tag="VHM69_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2740853.1"
                     /translation="MSSEHTGRHFTVAVFVVWEGKVLLHFHRKLRMWLPPGGHIEENE
                     LPDDAALREVFEETGVDVELVGERREDISDPVQLYRPAGVQLENIGPGHQHLDLIYFA
                     RPRGSTEIHADYSEDKVGWYGPEVWDTMAVNAEVTGWCERALSELDGYGSEQSRR"
     gene            complement(2052..2447)
                     /locus_tag="VHM69_10420"
     CDS             complement(2052..2447)
                     /locus_tag="VHM69_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740854.1"
                     /translation="MTLVLLILILIGALISLFAAVRALGAYLRFRRARAAFQSEVTDE
                     VERLAERTTELETNISALDTRAAELPIQISELQQSLSTLKVLTGALAASLRQVQKILS
                     YSALKTLSAARIGRILQSASAPRNRQHSG"
     gene            2520..2593
                     /locus_tag="VHM69_10425"
     tRNA            2520..2593
                     /locus_tag="VHM69_10425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2554..2556,aa:Met,seq:cat)
BASE COUNT          506 a          817 c          812 g          483 t
ORIGIN      
        1 gccggcaacg gggacgatgg cgtctcgatc gttgagggga ggggcaacag cgtcctctcc
       61 aacttcatct tctcgaacac cggcctcggg atgaacctct tccccgacgg cgtcacagcc
      121 aacgacaccg acgaccagga tactggagcc aacaacctcc agaacttccc cgtcataact
      181 tcggctataa agagctccac ctcccccttc ttcacgatca tccggggcac gctaaacagc
      241 aaccccaacc agacctacac catccagtgt ttcgtggcgg tgcctgaccc ctcaggccac
      301 ggcgaaggcc agatcccggt aggagcggat acgatccaga ccgatgccgg cggggacgcc
      361 gacgagaccg acagcttcac ctgcgccagc cccgttcctc aactcggaca agccgtgacc
      421 gtgacggcga ccaactcgac gacgggcgac acctccgagt tctccttgaa cgtcggcgtc
      481 actcctggcc cgtaagcgaa ggagcagata tccagttagt gctagcgcat tcgggcaccc
      541 tgcgcgaagt cgcggcatcg cattcaagct gcgtgaacct gttgccatga tgccccatct
      601 gacaacaccc cgtggctgtc gttccgggtg atcctgtcgt agattaggtt cgggacgttt
      661 ctcgtccccc cgcgtatggc cccgtgctct cgccgacgac ggcgtagagc gggtcggaga
      721 agaggcggcg ggggctgcga tccaagcccc tgacgctctc gaagttgccg gcgtctcgga
      781 ggtagcgttc gacgagttgc atgtgcccac ggtcatccaa ctggtgccag atggcgatcg
      841 ccttagtggg aaaacagcgg ttggagaatg tgatcaccac aggcgcgccc actttgagca
      901 cccgcccgac atcgcgcagc acctccacgg gccgggtcag gtaatctatc gagacgcaga
      961 tcccggcgcc ctcgaactca ccatctccga agggcaatct ggggtccgtg ttcaggttct
     1021 gtacgacgta tgagtccagc ctttcgttgc gacgcagctc cacctcgttc atacccagcc
     1081 ccatcacacg ccggtactcg acctctggtg gcaaatggct tacccaactg ctcatgagat
     1141 cgaggatctc accgccctca ggtaagaact ccctgtataa ctgcgtgacg gcggcgatgg
     1201 ccgcgtcatc tatgtgggtg acgagccgcg gcgttcggta gaacacctcg tcaggcgctt
     1261 cgtcggtacg ccggaaagct tcatctggaa cctggggcaa cagctcgcct ttctccggac
     1321 tgtcactacc aactcaaatt atgcaggttc gtagtgcctg tgtgtgacta cgcgtaccct
     1381 gcgggcctct cgtgtaacct gggacgtcat gagttcagaa cacacaggca ggcacttcac
     1441 ggtcgctgtc ttcgtggtgt gggaaggcaa agtgctcctg cacttccacc gcaagctgcg
     1501 gatgtggctc cctcccggtg ggcacataga ggagaacgag ctgccagacg acgccgcact
     1561 gcgcgaggtc tttgaggaga cgggagtcga tgtagaactg gtgggggaga ggcgagagga
     1621 catctcagac ccggtgcagc tatacaggcc cgcaggcgtg cagttggaga acatcgggcc
     1681 tgggcaccag catttagatt tgatctactt tgcgagacca cgaggctcga cggagatcca
     1741 cgccgactac agcgaggaca aggtcggctg gtacggcccc gaagtctggg acacgatggc
     1801 cgtcaacgcc gaggtcacgg gctggtgcga gcgggcactg tcagagctcg atggctacgg
     1861 gtcggagcag tcgcggcgat agccgcaggt cggacagacg agcttgcaat ggaggccgta
     1921 catctccgtg ccgcagacct cgcactcctc acccgaaggc ttcagtactt cagcggcttg
     1981 tgtctgctgc gcgctctcgt cttccatctt ctgaagtata cggatgcggc gcgtctgcga
     2041 caccggcggc tttagccgga gtgctgcctg ttcctcgggg ccgaggcgga ctggaggatc
     2101 ctgcctatgc gggctgcgct cagcgttttg agcgcgctgt aggagaggat cttctgcacc
     2161 tggcgaagcg aggcggcgag tgccccggtc aggaccttca gggtggacag gctctgctgg
     2221 agctcggaga tctggatggg aagctccgcc gcgcgggtgt cgagggctga gatgttggtc
     2281 tccagctcgg tggtgcgctc ggcgagacgt tcgacctcgt ccgtgacctc gctctggaag
     2341 gccgcgcggg cgcggcggaa cctcaagtac gccccgagcg cgcggacggc ggcgaacagc
     2401 gagatcaggg ctccgatcag gatcaggatg aggagaacga gggtcatctc gaaggctacc
     2461 gtaacacggc gctacctacc tttacaaacc tcggaccggt gctagaatct ttcccacgac
     2521 gcggggtgga gcagtctggt agctcgtcgg gctcataacc cgaaggtcgc aggttcaaat
     2581 cctgcccccg ctacttctcg gaggctccgc aaactgcg
//
LOCUS       DATAIR010000430         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_243469, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000430 DATAIR010000000
VERSION     DATAIR010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243469"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..600)
                     /locus_tag="VHM69_10430"
     CDS             complement(43..600)
                     /locus_tag="VHM69_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX2740855.1"
                     /translation="MAKVLVGITGGIAAYKAPGVIRRLKEAGHEVRTVATEAAFRFIP
                     EETLSIASGDYVHSNETWWEHSGRVEHVSLARWADLVLVAPATADSMARAAVGLGDDL
                     LSATILAGARAVLWAPAMNPEMWSSPATVRNVETLKSWGHRFVGPAEGLMASADEEPG
                     VGRLADETDIVAAVEEAISAEDHER"
     gene            complement(659..>2186)
                     /locus_tag="VHM69_10435"
     CDS             complement(659..>2186)
                     /locus_tag="VHM69_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2740856.1"
                     /translation="FLGAIDDITSEVCVPLFDQDTVVGILNLESVGGVRLTRADLRLT
                     SALGEQASIAISRARLYAQDQESKERLAHQAFHDSLTGLANRNLLMDRLEHAQARVRR
                     RTEVLAVLFMDLDDFKVINDSLGHAVGDRLLVAVAERLEACVRPQDTISRLGGDEFVA
                     LLEDLGDESDAVRVAQRIIEELQIPFTVDGNKLHTSISVGIAFSSAPAGDLLRAADTA
                     MYRAKENGKGRYEVCEQDMHHRALLRLQLENDLRAALDRGELAVHYQPEVDPRTGQVE
                     GVEALLRWRHPERGMIPPLEFLPLAEQTGLIVSIGEWVLREACMQARTWQETNKAPLF
                     VSVNLSVQQLKNIDFVADVLAETGLARPDLVLEITEDAVMEDAGYAIGALEELRRMGV
                     KLAIDDFGTGRSSLAYLKRLPVDYLKIDRSFIERLGEDSKGVEIVSGTLALARALNLR
                     TIAEGVETPAQLEWLREMGCDLAQGNLFTQPLDADAAQRFLQKSWAVAARGISNTRDP
                     "
BASE COUNT          350 a          706 c          717 g          413 t
ORIGIN      
        1 acgaggcttg cgcaagcttt tagactttgt agagtaacat tctcagcgtt catgatcttc
       61 ggcggagatc gcttcctcta cggcagctac tatgtccgtc tcgtcggcga ggcgcccgac
      121 accgggctcc tcgtccgctg acgccataag gccctccgcg ggtccgacga agcggtggcc
      181 ccagctcttt agcgtctcga cgttgcgtac tgtggcaggg ctgctccaca tctccgggtt
      241 catagcgggg gcccagagca cggctctcgc gccagcgagg atcgtggccg agagcaggtc
      301 gtcgccgagc ccgacggcgg cccgcgccat cgagtccgcg gtggcggggg cgacgaggac
      361 gaggtcggcc cagcgggcga gggagacatg ctcgacgcgg ccagagtgct cccaccaggt
      421 ttcgttggag tggacgtagt cgccggaggc gatggagagt gtctcttcag ggatgaaccg
      481 gaaagccgct tcggtggcga cggtcctgac ctcgtgtccg gcctctttga ggcgccggat
      541 cacccccggc gctttgtacg ccgcgatgcc gcccgttatc ccgacgagta ctttggccac
      601 gccggtagta tacagagcgc cgaaatcagg gacgcgtccg tggcgagaat ggcgcggatc
      661 atggatcgcg ggtgttcgat attccgcggg cggcgacagc ccagcttttc tgcaagaagc
      721 gctgtgcggc gtcggcgtcg agcggctgcg tgaagaggtt tccctgggcc aggtcgcacc
      781 ccatctccct gagccattcg agctgggccg gcgtctccac gccctcggcg atcgttctaa
      841 ggttcagggc acgcgcaagg gcgagggtgc ccgagacgat ctcgaccccc ttggaatcct
      901 ccccgagcct ctcgatgaag gagcggtcga tcttgaggta atcaacgggc agacgcttca
      961 ggtaagcgag cgaagagcgc cctgtcccga aatcgtcgat cgcaagcttc acgcccatgc
     1021 gcctgagctc ttcgagagcc ccgatggcgt atccggcgtc ctccatcacc gcgtcctcgg
     1081 ttatctcgag cacgaggtcg ggacgggcga gccctgtctc cgcgagcacg tcagcgacga
     1141 agtcgatgtt tttgagttgt tgcacggaga ggttcacgct gacgaacagg ggcgccttgt
     1201 tcgtctcctg ccacgtccgc gcctgcatgc acgcttcgcg gagcacccac tccccgatgg
     1261 atacgatcaa gccggtctgt tccgcgagcg gtaggaattc cagtggaggt atcattccgc
     1321 gctcgggatg ccgccagcgc aacagcgctt cgacgccctc cacctggccc gtgcgggggt
     1381 ccacctccgg ctggtagtgg accgcgagct ctcctctatc gagggccgcc ctgaggtcgt
     1441 tctcgagctg cagacgcagc agcgccctgt ggtgcatgtc ctgctcgcac acctcgtaac
     1501 gacccttgcc gttctccttg gcgcggtaca tggcggtatc ggcggcgcga aggaggtccc
     1561 ccgcgggggc cgagctgaag gctatgccga cgctgatcga ggtgtgcaac ttgttgccgt
     1621 cgacggtgaa tgggatctgt agctcttcta tgatgcgttg ggcgacccgc accgcgtcgc
     1681 tctcatcccc gaggtcttcg aggagggcaa cgaactcgtc gcccccgagg cgtgagatgg
     1741 tgtcctgggg cctgacgcag gcttcgaggc gttcggccac agcgacgagc aggcggtccc
     1801 cgacggcgtg gcccaaggag tcgtttatga ccttgaagtc gtccaggtcc atgaagagca
     1861 cggcgagcac ttccgtacgc ctgcgtaccc tggcctgggc gtgctccagc ctgtccatca
     1921 acaggtttcg gttggcgagc cccgtcaacg agtcgtggaa ggcctggtgg gccagccgct
     1981 ccttgctctc ctgatcctgc gcgtatagcc tcgcccggct tatggcgatg ctcgcctgct
     2041 ccccgagggc gctcgtcagc cgcaggtcgg cccgcgtgag cctcacgccg cccacgctct
     2101 ccaagttgag gatgccgacg accgtgtcct ggtcgaacag aggaacgcac acttccgagg
     2161 ttatgtcgtc tatcgcgcca aggaaa
//
LOCUS       DATAIR010000431         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_27297, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000431 DATAIR010000000
VERSION     DATAIR010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27297"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VHM69_10440"
     CDS             <1..458
                     /locus_tag="VHM69_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX2740857.1"
                     /translation="LTLSVLIILYGAYAVVDGVFAIVVGLRADSGTRRWLLMAEGALG
                     ILAGLIAVFWPGVSAVVLLYIIAFWAIFGGLLRIVGAVLLRQWTMAISGALWMLLGIV
                     LFVLPSAGLISLAWLIGIFALGAGITLVALALRVRSQGSGEPGGSSRTT"
     gene            510..905
                     /locus_tag="VHM69_10445"
     CDS             510..905
                     /locus_tag="VHM69_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740858.1"
                     /translation="MTKVRALVGGLAVLVLLAFAAGCGLVSDQAKQEAKKKIEAKGQQ
                     ARQEAKKKVEARKQEVTKRVEAGQEDVKKKVDDLKEKVEAGQEDLTKKVDDVQKRMDD
                     LHNEMDDVQAKLDELLKEVEAQEKQNQKE"
     gene            complement(1094..1792)
                     /locus_tag="VHM69_10450"
     CDS             complement(1094..1792)
                     /locus_tag="VHM69_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740859.1"
                     /translation="MSEHGSISGVWREALAIIRCYPSAVLVPGAVLGAVAEAPAYFIA
                     DRILLEQLLTYLAAAFAYYLYLAYAEGITTDYEHGEDLIVSRGRLARFFEAATFVPRV
                     LVAALVTVSLASAATGLLMVPGMWLYTRWSLSVPVICKEDMRPVASLKRSSALVRGRF
                     WFVLGTATLAFVSEEAVIHVGALATGSITGSHTWGEWVGGSIVAALAIPLAALTTSVA
                     YSRVVKLSGPVEGS"
     gene            complement(2304..3581)
                     /locus_tag="VHM69_10455"
     CDS             complement(2304..3581)
                     /locus_tag="VHM69_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2740860.1"
                     /translation="MVDLAVLATVVFVFGLVSRRLEGTVLTAPLVFVAAGVLLGPAGL
                     GLAEFDLDDHSVLLLGEIALAIVLFTDAAGIKLSALRQNKALPLHLLGIGMPLTIALG
                     TIVAMLVLTDLTFWEAAIVGTVLAPTDAALGQTVVSNPRVPVRVRQTLNVEAGLNDGL
                     SVPFLALFLTLAVAEEELQPASFWIRFTLEQVGLGTLVGISVGLVGGWLVSLASRKEW
                     MTGSFQRIAPLALAIIAWALADQIGGNGFIAAFVGGLVLGPTVERVGEQLIRFTEAEG
                     QLLNLSVFFVFGVLVVGLMEPLSWEVALYALLSLTLIRMLPVAASLIGTKLRGVSILF
                     IGWFGPRGLASIVLGLIVVSEAPLLPGREEIELVVALTVLLSVLLHGVTAAPLSARYA
                     RKVEGMAADAPEKQGTVELPTRAGSAKVADEAE"
     gene            complement(3764..4018)
                     /locus_tag="VHM69_10460"
     CDS             complement(3764..4018)
                     /locus_tag="VHM69_10460"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2740861.1"
                     /translation="MAHASVDTKSNARSAGSAAPKRCAGYAVYDPLGQKIGVVEEVFA
                     NRDDKPQYVRVRIGFFGGRSVLIPVQFVETDRERRTLTLK"
     gene            complement(4278..>4638)
                     /locus_tag="VHM69_10465"
     CDS             complement(4278..>4638)
                     /locus_tag="VHM69_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740862.1"
                     /translation="VTFAAIMIFVVAGFEALAALFAFAGTGWWATETGNLVYANFVFW
                     GIVDSIIALIALYAGIDLLRGGTFGLGAGYVFAVVGAIRWLFVIPAAPVLAVVVIALC
                     VLVIYGLATYSDRLEST"
BASE COUNT          989 a         1395 c         1426 g          828 t
ORIGIN      
        1 gccttactct ctcggttctg atcatactgt acggtgccta cgccgtggtc gacggggttt
       61 tcgccatcgt cgttgggctc agggcggaca gcggaacacg caggtggctg ctgatggccg
      121 agggtgcatt gggcatcctg gccggtctta tcgcggtctt ctggccgggc gttagcgcgg
      181 tcgtgctgct ctacatcatc gctttctggg ccatcttcgg cgggctcttg aggatagtcg
      241 gggccgtatt gctgaggcag tggaccatgg ctattagcgg cgccctgtgg atgctcttgg
      301 gcatagtctt gttcgtcttg cccagtgcgg gattgatctc cctggcgtgg ctcatcggca
      361 tctttgcgct cggagccggg ataacgctgg tagctttggc acttagggta cggagccagg
      421 gctccggcga gcctggtggg agcagcagaa cgacctaaaa ccaccggagg acgtctggtc
      481 gaacggaagg gtggagaggc gatgcggaaa tgacgaaggt tcgggcgttg gtggggggat
      541 tggccgtgct agtgcttctg gcgttcgcag cgggatgcgg gctggtttcc gaccaggcca
      601 agcaggaggc gaagaagaag atcgaggcca aaggtcagca ggcccggcag gaagcgaaga
      661 agaaggtcga ggccagaaag caagaggtga cgaagagggt cgaggccggc caggaggacg
      721 tgaagaaaaa ggtagacgac ctgaaggaga aagtggaggc tggccaggag gacctgacga
      781 agaaagtgga cgacgttcag aagagaatgg acgacctcca caatgagatg gacgatgtgc
      841 aggcgaagtt ggacgagctg ctgaaggaag tagaagccca agagaagcag aaccagaagg
      901 agtagacgaa gcagacgcgt gactcatccg gggcgagact gactaggagt accagcgatc
      961 aatccacaaa catcatagct acagttagca gaagcccgac tttcggagaa caccgtagcc
     1021 ccggcgttgg ctggggtctc ctgcgcacaa cgcgtgcagg agcttccaag atgttcgtgc
     1081 gcaaccgttc gtttcaagag ccttcgactg gcccagagag tttcacgacc ctcgaataag
     1141 ccaccgaggt cgtaagggcg gccagcggta tggccagcgc cgcgacgatc gagccaccga
     1201 cccactcacc ccaggtatgc gatcccgtga tcgagcccgt cgcaagcgca cccacgtgga
     1261 ttaccgcctc ctctgagacg aaggcgaggg tggccgtccc gagcacgaac cagaagcgtc
     1321 cccgcaccaa cgcgctgctt cgcttcaggg acgcaacagg tctcatgtcc tctttgcaga
     1381 tcacagggac agacagcgac catcgcgtgt aaagccacat ccctggtacc ataagcagcc
     1441 ccgttgccgc gctcgccagg gagacggtga ccagcgcggc cacgaggaca cgcggtacga
     1501 aggtagccgc ttcaaagaac cttgcaagcc tgccgcgcga tacgatgaga tcttccccat
     1561 gctcgtaatc ggtcgtgatt ccctcagcgt aggccaggta gaggtagtaa gcgaatgcgg
     1621 cggccaggta tgtcaggagt tgctccagca gtatacgatc cgcgatgaag taagcaggag
     1681 cttccgcaac agcgccgagc acagccccgg gcactagcac cgctgaaggg tagcagcgga
     1741 tgatcgcgag ggcttcgcgc cacacacctg agatggaacc gtgctcgctc atgcaccttc
     1801 ttttgggagt tcgcacctcc tcaacaacct accagtccct atgtagcctc gcggccctat
     1861 tcacccaacc tcgtagactt cgatctttag ccaattaaga ttgaaggggt aggatacact
     1921 cctcaccagt cccctctctt cctggctgcg gtgctaaacc ccgctttcaa ctggctgcag
     1981 cggcgccaca ggttgatcaa acgccccatc gctatagcgc tcacctacct tggagtggtg
     2041 gttgcgctgc tgtttgtggt cgggatcttc ttgccgctgc ttgtagagca gatcaacggt
     2101 ctgatcgact tcgttgtcgc tgtggccaag gctcctgagg gaccgacaga atacatcaag
     2161 gggctcgccg aacaaaacgg gctcgggggg ctcttcgaga gggtttcagg ttctgcccct
     2221 gtgcccctct gtgcggcctt tccctccttc atatgtgtag ctactgctgt tcctcatggt
     2281 accgggctcg ttccatctgg atctcattcg gcctcatccg ctactttggc ggagcccgcg
     2341 cgggtcggca gctctaccgt tccttgcttc tccggcgcgt cagcggccat cccctcgact
     2401 ttgcgggcgt acctggcgga gagtggagcg gcggtaaccc cgtgcaacag cacgctgagc
     2461 agaaccgtca gtgccaccac cagctcgatc tcctcgcgtc cgggaagcag tggggcctcc
     2521 gacaccacga tgaggcccaa cactatcgag gcgagccccc gtgggccaaa ccaacctatg
     2581 aacaggatgg agacgccgcg cagcttggtg ccgatcaaag aagctgcgac ggggagcatg
     2641 cgaatcaagg tcaagctgag gagggcatac agagcaacct cccagctcaa gggttccatc
     2701 agcccgacaa cgagcacacc gaagacaaag aacacgctca ggttgagcag ttgcccttcg
     2761 gcctcggtga agcgtatcaa ttgctccccg actcgctcca cggtcggtcc gagcaccagg
     2821 ccaccgacga aggcggcgat gaagccgttg cccccgatct ggtcagccag cgcccaggcg
     2881 atgatcgcca aagccagcgg ggcgatgcgc tggaacgatc cggtcatcca ctctttcctc
     2941 gatgccaggc tgaccagcca gcccccaacc aggccgacgc tgatgccgac gagagtgcca
     3001 aggccaacct gctccagggt gaagcgtatc cagaagctcg ctggttggag ctcctcttcg
     3061 gccaccgcca gggtcaagaa gagtgccagg aagggcaccg aaagaccgtc gttcagaccg
     3121 gcctcgacgt tgagggtctg gcgcacacgc acggggacgc gcgggttgct cacgaccgtc
     3181 tgtccgagcg ccgcgtcggt aggggcgagg acagtgccca caatggcggc ctcccagaaa
     3241 gtgagatcgg tgagcaccag catcgcgaca atggtcccta gcgcgatggt gagcggcata
     3301 cctatgccga gaaggtgcaa tggcaacgcc ttattctgtc ttagggctga gagcttgatc
     3361 cccgcggcgt cggtgaacag cacgatcgcg agagcgatct cgcccagcaa gagcacgctg
     3421 tgatcgtcga ggtcgaattc ggccaggccg agacctgccg gaccgaggag gacacctgcc
     3481 gcgacaaaca cgagaggcgc cgtgaggacc gtcccctcca gccgccgcga aacgaggccg
     3541 aagacgaaga cgacggtggc cagcacggcg agatcaacca ccgtcggctc cagggaagtt
     3601 cgacgaggac gggtgcacga gtgcgcagcg caagacgagg ccgtgtcgag gaggagacct
     3661 tcccccgagg ttacccgttg tcagcgccgt cctgtgacag aggcgaaggc cgcatagagt
     3721 cgtcccggat ggagtcattc cttttgtgtt tccttctatg caactatttc agggtaaggg
     3781 tccttcgctc ccgatctgtc tcgacgaact gcaccgggat taacacggac ctaccaccga
     3841 agaaccctat ccgtaccctg acgtactggg gtttgtcgtc cctgttcgcg aacacttcct
     3901 ccacgacacc gatcttctga cccaaaggat cgtagaccgc gtatccggca cacctcttgg
     3961 gtgccgccga acctgcactc ctcgcattgg atttagtgtc taccgatgcg tgggccacat
     4021 cctactcctt tcgggatggc tgaatatctc gatctccgca agagggttga gcccaccgtc
     4081 tccgttaacc tgtagcgcgt cttagctggc gcccgatcac cgcaccgggc ggtctcctat
     4141 gcgcccttga tgtagatgcg tagagtagtt ggagcccggg atcatagggc cgatgcgggc
     4201 gggaattccc gcccgcatcg ctagcaccca gccgttagat catgtccggt ggctgacaag
     4261 cctatacctg attgcactca ggtactctcg agtcggtcgg agtaagtggc aagcccgtag
     4321 atgaccaaca cgcaaagcgc gataaccacc actgctagca caggcgccgc cgggatgaca
     4381 aaaagccacc tgatagcgcc tacgacggcg aagacgtagc ccgcaccaag gccaaaggtt
     4441 cctcctctca ggaggtctat gcccgcgtac agcgcgatga gcgcgatgat cgagtcgacg
     4501 ataccccaga aaacgaagtt cgcgtacacg aggttgccag tctcggtcgc ccaccagcca
     4561 gtgcctgcga acgcaaagag tgccgctaga gcctcgaagc ctgccaccac gaatatcatg
     4621 atggcggcga aggtcacc
//
LOCUS       DATAIR010000432         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_335253, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000432 DATAIR010000000
VERSION     DATAIR010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335253"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..960)
                     /locus_tag="VHM69_10470"
     CDS             complement(400..960)
                     /locus_tag="VHM69_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740863.1"
                     /translation="MSVRDQERKAQQAERRMHDVMRRADREVGKRIEARAHEASLRAN
                     EVKRRIERGDVDPRPLPGMREDSMPSIASMPSMESSPSMKSIESADYARGVSEEAQQA
                     VEDGDVMMKDMPLEGTGMDPEAAHEDLAQRVTEETGSGGDPHKHAERKADEIAGQITE
                     DTGADGDMQVHAGRKVEQMGQEMFED"
     gene            complement(1371..1568)
                     /locus_tag="VHM69_10475"
                     /pseudo
     CDS             complement(1371..1568)
                     /locus_tag="VHM69_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(1629..>1902)
                     /locus_tag="VHM69_10480"
     CDS             complement(1629..>1902)
                     /locus_tag="VHM69_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740864.1"
                     /translation="EFKRQLVKAARMYAMCVKAEVAAPMDVVGIAMAAFEDMPLKQAL
                     VLVRSNEQNVKDLAWALENSNSAQEFEQRVKEIKSLPHDHSDRESR"
BASE COUNT          271 a          640 c          522 g          469 t
ORIGIN      
        1 ggagcaatcg ccacattctc cggtcttcga agggtgcgat cccccctgcg tccctcgctt
       61 cgaacactaa acaggacctc ctgtgtcgag cgcattctat gctggcccgc gaaactctgt
      121 aaacctcgtt atcccacaac cgcaatacct ggaggaacag ggcgaataca gagccatatg
      181 tgggctcggg ccgtttagcc gtagcgacac cagcgtgcac ggcctcacgc cctggagtgc
      241 ctcctggtcg agcccgttgt ccggcggcac cctagctctg gatggcggcg tggcgctcga
      301 aagtaaacta ccgtcagggc ttggcctcga ccctggaggc ttcgtcactg catttctcga
      361 aagctcgccg gttcttcgaa tgaaggcttc ggagtgcctt cagtcctcga acatctcctg
      421 tcccatctgc tcaaccttac gccctgcgtg tacttgcatg tccccgtccg cgcctgtgtc
      481 ctcggttatc tgcccggcta tctcgtccgc cttgcgctcg gcgtgcttgt gcggatctcc
      541 acccgaacca gtctcctcgg tcactctctg cgcgaggtcc tcgtgcgcgg cctcggggtc
      601 catgcccgtc ccctcaagcg gcatgtcctt catcatgacg tccccgtcct ccaccgcttg
      661 ttgcgcctct tcgcttaccc cgcgtgcata gtcggccgac tctatggatt tcatggacgg
      721 cgaagactcc atggatggca tcgacgcgat ggacggcatc gagtcttccc gcatgccagg
      781 cagggggcgc gggtcgacgt cgcccctctc gattctcctc ttcacctcgt tcgccctgag
      841 ggaggcttcg tgcgcccgcg cttcgatcct tttcccgacc tctcgatccg ccctgcgcat
      901 gacatcatgc atcctgcgct cggcctgctg tgccttacgt tcttgatccc gtacgctcat
      961 gcgacttccc tttcaggctg cgagtagtac aaaggagatt ctgctcccgt gccacgaaga
     1021 cagcttgcgc aggcgacgtg ttctcgggtc tgctaggcat tggggcgttt tctcgttttc
     1081 ggcggtttgg gtggtttccg tggcttggac ggcttcttgc ccgaggcgtc ggctgatccg
     1141 cccttctcag aactcgtctt cctctttatg ccctcacccg ccagcttcga gaggcgggta
     1201 atgtccatgc ccaggattct ggagatgcgg gctatatcct cgactccctc aacgttgagt
     1261 tgccagttga tctcgcgtcc gccgtcccga gatccggtgg tcttcttcga ccctctggta
     1321 ccttttctgg atgagccggg tcgtctcttc cgcgaagtgg ggtgggatct ttggtcgtat
     1381 tcacggcgct tgtcggggtt cgagaggatc tcgtaggcct gctggatctc cttgaagcgc
     1441 tcctcggcct cgatgtcgcc gggattcgcg tcaggatgat actcgcgggc cagccgccgg
     1501 taggctttgc ggacgtcttc ttcggacgct cccctcgaca cgcccaacaa cccgtaggga
     1561 tccttcacct tctagcctcc caccccgtcg tgactatgtt cccgacgagc atcgcggagc
     1621 cttccggcct atcttgattc gcggtcgcta tggtcgtgtg gcaggctctt tatctccttg
     1681 acccgttgct cgaactcctg agccgagttg gagttctcga gcgcccaggc caggtctttg
     1741 acgttctgtt cgttcgatct cacgagtacc agcgcctgct tgagcggcat gtcctcgaag
     1801 gccgccatcg cgatgccaac cacgtccatc ggtgcggcga cctcggcctt cacgcacatg
     1861 gcgtacatcc tggccgcttt cacgagttgc ctcttgaatt cc
//
LOCUS       DATAIR010000433        12533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_27416, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000433 DATAIR010000000
VERSION     DATAIR010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27416"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1731)
                     /locus_tag="VHM69_10485"
     CDS             complement(4..1731)
                     /locus_tag="VHM69_10485"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2740865.1"
                     /translation="MFRKVLIANRGEISVRIVRALREMGIGSVTVYSDADRASLHTSI
                     ADEAYHIGPTPATQSYLNVEKLIEVAHKSGAEAVHPGYGFLAESAAFARAVEEAGLVW
                     IGPHPEAIEAMGSKVESRRIMARAGVPMVPGTEDSIGSAKEVLVFGERQGFPVAVKAT
                     AGGGGKGFALARNASEAESAFGRARREGVAYFGDGSVYVEKYLPAPRHVEIQVVSDKH
                     GNAVHLGERDCSIQRRHQKLIEECPSPAIDPAMREAMGEAALAAAAAVDYDNVGTVEF
                     LVQGDEFYFLEMNTRVQVEHPVTEEVTGVDVVKTGVRIAAGDPLPFSQEDVSWRGHSI
                     EVRLNAEDVSRDFVPSPGFVSVYEEPGGPGVRVDSSLRGPGVVAESYDPLFAKLIVRG
                     ADRKEALARLGRALAEFRIEGISTTLPLFRAILDDEVFASGNYTTGFIAERMEGLQIE
                     SAPAAERGAVPEKSAREVEVEVNGKLFRVKVYEDARGATGPPRRKRGETRRASASEGS
                     VSAPMQGTIVKVLVKEGQEVAADEPVCILEAMKMESEVRSQKAGTVSEIRIQAGQTVR
                     SGDTLIVVE"
     gene            complement(1731..1937)
                     /locus_tag="VHM69_10490"
     CDS             complement(1731..1937)
                     /locus_tag="VHM69_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740866.1"
                     /translation="MKIEPSPTYEETAAIVAALETFSAEDPEDLSSLKRSRWRLAGLL
                     GHEVEPGMKLEGSLWSYSRWEGMV"
     gene            complement(1934..3508)
                     /locus_tag="VHM69_10495"
     CDS             complement(1934..3508)
                     /locus_tag="VHM69_10495"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2740867.1"
                     /translation="MQGERLGEETTAGKIEGLEKLREAAAHPAPEAAVERQRERGKLT
                     ARERIELLLDEGTFVELDRYRVHRSYNFGLEENRPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVYAEKICKVMDLAISTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVVPQISVIVGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEDVSF
                     EDLGGAATHNSRSGVAHFSAPDEETCFDDVRYLLSFLPENNLESAPYFRPTDDPGRMD
                     EDLAHLIPDSPQRPYDIRGAIVSTMDDGEFFEVQEGWAQNLVVGFARLDGRAVGVVGN
                     QPSVLAGTLDINASVKGARFVRFCDAFNIPLVTFVDVPGFMPGTDQEWGGIIRHGAKL
                     LYAFSEATVPKMTVITRKAYGGAYDVMCSKHIGADVNVSWPTGEIAVMGAPAAVGVIF
                     RRQISEADDPDAKREELISDYKEQFNNPMVAAEMGFIDDVIDPRETRPYLIKALQMIR
                     TKRPDRPPRKHGNIPL"
     gene            complement(3569..3919)
                     /locus_tag="VHM69_10500"
     CDS             complement(3569..3919)
                     /locus_tag="VHM69_10500"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX2740868.1"
                     /translation="MKFKIDENLPVEVAGELLAAGHEAVTIHDQQLVGSGDHALSEVC
                     RSEGRVLVTLDLDFADIRTYPPENYPGLIVLRLGRQDKPYVLEVFQRLLRAIDGEPLE
                     GCLWIVEENRIRIR"
     gene            complement(3928..4155)
                     /locus_tag="VHM69_10505"
     CDS             complement(3928..4155)
                     /locus_tag="VHM69_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2740869.1"
                     /translation="MDWREYIIVDPEVCHGKPCVKGSRVMVSVILDNLAVGQTPEEIM
                     RSYPSISREAIQASIAYAADLARERFVSMPA"
     gene            4407..5513
                     /locus_tag="VHM69_10510"
     CDS             4407..5513
                     /locus_tag="VHM69_10510"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2740870.1"
                     /translation="MQVFEKLAEYRYEQLVFCHDKATGLRAIIAIHDTTLGPALGGCR
                     MYPYASEEDAIVDVLRLARGMTYKAAASGLNLGGGKSVIIADPDTDKSEALFRSFGRY
                     IETLGGRYIVAEDVGTSTEDANYIRVETSHVVGVDVTRGGSGDPSPLTALGVLQGMRA
                     CAEDVFGTTSLEGRVVAVQGLGHVGYHLCRLLDDDGANLIVTDVNKAAVERVVTEFGA
                     KAVETDEILTIPCDIFAPCALGAVINDDTLPTLDCRIVAGSANNVLAESSHGEALVER
                     GILYAPDYVINAGGLINVADELEGYNERRATKRVMRIEDRIRRIIAISKRDGVPTNVA
                     ADTMALERIAAISSMERLHTGHPYGQLVRRRETI"
     gene            complement(5664..6476)
                     /locus_tag="VHM69_10515"
     CDS             complement(5664..6476)
                     /locus_tag="VHM69_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX2740871.1"
                     /translation="MLELQRNVIFGQFVDTGSFIHRMDPRVKIAATFLVIVASFLIDS
                     FLGFAIVTPLLVLIQIVSRIPPGYVLRGSRLFLGFLVFILAFQVLFYPGDVPESGYLW
                     RWGILSVSTEGLYTAGIIAMRVILLYYVITMLMLTTSLVDLTNGLELIFGPLQRLRIP
                     VNELVLVSVIAIKFVPIFIEEMERLARAQTARGVPFDEGGAITRARRLGRLLVPIFIS
                     GFARADTLTIAMNTRSYRGGRYRTKLHRMRASLSDWLVLALVTAWLIVAWMA"
     gene            complement(6476..7318)
                     /locus_tag="VHM69_10520"
     CDS             complement(6476..7318)
                     /locus_tag="VHM69_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2740872.1"
                     /translation="MRSFSHVYLEGTPRASEAVRDVSFQIHRGERVGVVGATQSGKST
                     VVDSFAHLLRVRPGQVFYNGEDIAARGYDKGRLRREVGIVFQRPDSQIIEDVVGADVA
                     FGPTAAGLSASETRTRVEESLNTMGLPYPDYRLRYVHALSGGQRRRVAIAGVLAMRTP
                     VLVLDEPMAGLDPRGRRQLLELLIKLQRERELTLIVCSASLTDASLLCDRLIVLDRGT
                     VVMDGPVREVLSEADRLAELDITLPEPVVGAREMRKLFPDFPTELLTEEELESELLKR
                     LGAA"
     gene            complement(7351..8196)
                     /locus_tag="VHM69_10525"
     CDS             complement(7351..8196)
                     /locus_tag="VHM69_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2740873.1"
                     /translation="MIEIDGVSFTYRTGSTDAVPALRGLDLKIEPEQLVAIIGHNGSG
                     KSTLVKLLTAVLHPTEGEIRIDGVPVDHEHQWEVRRKVSVVFQDPDDQLVMNRVEDDV
                     AFGPENLGLSREEILERVESSLRALGLEEIRGRLVEDLSPGQKQRVAIAGALAMRPHF
                     LILDEPTSLLPVPVAMRLISTVKELNRSGNMGVLHVTHSMYEAVLFDRVVVMDEGLVV
                     LDGTPAEVFRRVEELREVGLDVPLAASLAHRLRARGVPLGDGILNTEDLRAALSAAVD
                     PSIYG"
     gene            complement(8206..8808)
                     /locus_tag="VHM69_10530"
     CDS             complement(8206..8808)
                     /locus_tag="VHM69_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX2740874.1"
                     /translation="MSTGSGSQGRGSIFSLDTRQIVVAGIIGGIALFLGFTRLGFIPV
                     PIPLIGNATLMHIPAIVGGVLEGPIVGLLAGAIFGIFSFLQAENPVFLNPIVAIVPRL
                     FIGVVAWAVFVGLRRWSVDLAAAVSGLLGSFTNTVGVLGLAILFGLLPPAVIPAVLPQ
                     AIAEAVLAAVVTVVIVRGVMLFRSGRTTAPETESDEERRY"
     gene            complement(8898..9680)
                     /locus_tag="VHM69_10535"
     CDS             complement(8898..9680)
                     /locus_tag="VHM69_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2740875.1"
                     /translation="MFDYGSMFDLTDKTALVVGAGSGIGEASAHGLAAFGARVVCADV
                     NGGAADDTAQEIRSREGKADAIELDMTDPESVRSAVERVGTPDVLVSTPSVNVRKPVL
                     EITDEEFDKVVELNLKGTFRLIRDFGRGMAERGSGSIIAFSSIRGQVVEPGQGVYAAT
                     KAGTVQMLRALASEIGPRGVRVNAIAPGIVETPLTAQIKDSPDWYDSYAAKSILGRWA
                     RPHEMVGMVVYLASEASSYVTGGFMLVDGGWTAADGRFTPPL"
     gene            complement(9673..10947)
                     /locus_tag="VHM69_10540"
     CDS             complement(9673..10947)
                     /locus_tag="VHM69_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2740876.1"
                     /translation="MTEPDVDAGANAAFRRVLEDVLGRIDREELVRLAQELVRIPSVY
                     LPEEPEGNEARVAGFVADYLERAGFGVQVDVVAPGRPNVWAVWEGDSPGRTLLFEAHT
                     DVVTEGNAEDWDHQPFAAERDRGRIYGRGACDTKGNLAAAVTAVCAIRDSGVPFPGRL
                     ILCHPVDEEGMMTGVKAFIERGHAEGVDAAVICEPEENHLCIRQKGALRVEVTVRGQM
                     AHGAMPRSGVNPVTRAARFVVAVEELESEEVERHGEDPFLGYPSLTPTILMGPDYGEP
                     QINVIPASAYVALDIRTVPSQSHEELIERLEGILARLASEDPDFEATLEVIEERPPTE
                     TPKDEPLVRAMAEAYTHLTGEEPRYNGVPGATDGTFLHAWAAIPIVTTGAGNREIPHH
                     TDEWVDEEELLTTCKLYAATAMFYAHGKDAHV"
     gene            complement(11224..11775)
                     /locus_tag="VHM69_10545"
     CDS             complement(11224..11775)
                     /locus_tag="VHM69_10545"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX2740877.1"
                     /translation="MSPRTRATIEDLYRVPETGKAELVNGEIVLMAPTGGIPGRAGGE
                     IYVSLRDYERRTGRGYAFPDNVGFTVNLPHRGSFSPDAAFHAGPLQGGRFLDGAPIFA
                     AEVRSEHDYGERTEQEMAEKRTDYFAAGTLVVWDVDVLHERVVHVYRADEPDRPATYQ
                     SDDVAEAEPALPGWTMLVRDLLP"
     gene            complement(11985..>12533)
                     /locus_tag="VHM69_10550"
     CDS             complement(11985..>12533)
                     /locus_tag="VHM69_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX2740878.1"
                     /translation="EGMKLSYLPFIVKAVVESLKENPVLNSVWDEDRIVLRKRINVGV
                     AVDLEDALIVPVIPDADELNILGLARRIDDLVRRARDRQLGTEDISGGTFTVNNPGSL
                     GSVVSTPIINHPQAAILSAEAVVKRPVVIDDGGTDAITIRSMMNLEVSFDHRILDGGA
                     ALKFLNAVKRRLEDYGPESGIG"
BASE COUNT         2304 a         4085 c         3798 g         2346 t
ORIGIN      
        1 gccttactct acgacgatca gggtgtcccc tgaccgcacc gtctggccgg cctggatacg
       61 gatctcggag acggtgccgg ccttctggga acggacctcg ctctccatct tcatcgcctc
      121 gaggatgcac acgggctcgt ccgcagcgac ctcctgcccc tccttcacga gcaccttgac
      181 gatggtcccc tgcatcggcg ccgaaactga accctcgctg gccgacgccc gcctggtctc
      241 accacgcttg cgccgcggag gccccgttgc gccgcgcgcg tcctcgtaga cctttaccct
      301 gaagagctta ccgttgacct cgacctcgac ttcgcgcgcg ctcttttcgg gaaccgcgcc
      361 cctctctgcg gcgggcgcgg actctatctg aagtccctcc atcctctctg cgatgaagcc
      421 tgtggtgtag tttccggagg cgaagacctc gtcgtcgagg atcgcgcgga aaagaggaag
      481 ggtcgtcgag ataccctcga tcctgaactc agctagcgcc cgcccgagcc gcgcgagggc
      541 ttccttcctg tccgcgccgc gcacgatgag cttggcgaac agcgggtcgt aggattcggc
      601 cacgacgccg ggaccgcgca acgaagagtc cacccttacg ccggggccgc ccggctcctc
      661 gtagacgctg acgaagccgg ggctcgggac gaaatctctc gacacgtcct cggcgttcag
      721 acgcacttcg atggagtggc cgcgccagga gacgtcttcc tgcgagaaag gcaaggggtc
      781 gcccgcggcg atgcggacgc ctgtcttcac gacgtctacg ccggtcacct cctcggttac
      841 aggatgctcg acctggacgc gggtgttcat ctcgaggaag tagaattcgt cgccctggac
      901 caggaactcg acggttccca cgttgtcgta atcgacggcc gctgcggcgg cgagtgcggc
      961 ctcacccatc gcctcgcgca tagccgggtc tatggctgga ctcggacact cttcgatcag
     1021 cttctggtgc ctacgctgta tcgagcagtc cctctcaccc agatggacgg catttccatg
     1081 cttgtcgctg acgacctgta tctcaacgtg gcggggggca ggcaggtact tctcgacgta
     1141 gaccgagccg tcgccgaagt acgccacgcc ctccctgcgc gcccgcccga aggcgctttc
     1201 ggcctccgag gcgttccgcg ccagggcgaa acccttgccg cccccccctg cagtggcctt
     1261 cacggcgaca gggaagcctt gcctctcccc gaagacgagg acctctttcg cggagccgat
     1321 agaatcttcg gtgccaggca ccatcgggac gcccgccctg gccatgatcc tgcggctctc
     1381 caccttgctc cccatcgcct ctatcgcttc cggatggggc ccgatccaga cgagcccggc
     1441 ctcctcgacg gcccgcgcga aggccgcgct ctcggccagg aagccgtagc cagggtgcac
     1501 ggcctccgcc ccactcttgt gcgcgacctc gatcaacttc tccacgttga ggtagctctg
     1561 tgtggcaggc gtaggaccta tgtgatacgc ctcgtcagcg atacttgtgt gtaatgacgc
     1621 cctgtccgcg tccgagtaga cggtgacgct acctatgccc atctcacgca gcgcgcggac
     1681 tatgcggacg gagatctcac cccggttggc gataagcacc tttctgaaca ctagaccatg
     1741 ccctcccacc tggagtagga ccagagggag ccttccagct tcatcccggg ctccacctcg
     1801 tgtcccagaa gtccggcgag ccgccagcgg ctacgcttca gggaagagag gtcctccggg
     1861 tcttccgcag aaaacgtctc cagcgcggcg acgatcgccg ctgtctcctc ataagttggc
     1921 gacggttcga tcttcaaagt gggatgtttc cgtgtttgcg gggcggacga tcggggcgtt
     1981 tggtacggat catctgcaga gctttgatga ggtaggggcg tgtctcgcgc gggtctatca
     2041 cgtcgtctat aaagcccatc tccgcggcga ccatcgggtt gttgaactgc tctttgtagt
     2101 cagagatcaa ctcctcgcgt ttcgcatcgg gatcatcggc ctccgagatc tgacgcctga
     2161 agatgacgcc gaccgccgcg ggagccccca tgaccgcgat ctcacccgtc ggccacgaga
     2221 cgttgacgtc ggccccgatg tgcttggagc acatgacgtc gtaagccccg ccgtacgcct
     2281 tcctggtgat gacggtcatc ttgggcaccg tcgcctcgga gaaggcgtac agcagcttgg
     2341 ctccgtgacg gatgatgcca ccccattcct ggtcagtgcc aggcatgaac ccaggcacgt
     2401 caacgaacgt gactagcgga atgttgaagg cgtcgcagaa gcgcacgaac cgcgcgccct
     2461 tgacgctcgc gtttatatca agcgtccccg caagcaccga gggctggttc cctacgacgc
     2521 cgacggcccg cccgtcgagc ctggcgaagc cgacgacgag gttctgtgcc cagccttcct
     2581 gaacctcgaa gaactccccg tcatccatgg tgctcactat cgcgccgcgg atgtcgtagg
     2641 ggcgctgggg agagtccggt atcagatgag cgaggtcctc gtccattcgc ccggggtcgt
     2701 cggtgggacg gaagtacggg gcgctctcca ggttgttctc ggggaggaaa gagagcaggt
     2761 agcgaacgtc gtcgaagcac gtctcctcgt cgggggccga gaagtgggcg acgcctgacc
     2821 tggagttgtg ggtggccgcg ccgccgaggt cctcgaaaga gacgtcctcg cccgtcacgc
     2881 tctttatcac gtcggggccg gtgatgaaca tatggctcgt ctcctcgacc atgaagacga
     2941 agtccgtgat cgccggcgag tacaccgcgc cgcctgcgca aggcccgacg atgacgctga
     3001 tctggggtac aacccccgag gcgagaacat tcctgtggaa gatgtcggcg tagccggcca
     3061 gggagacaac gccctcctgg atacgggctc cgccggagtc gtttataccg atgatcgggc
     3121 atccagtcga gatggcgagg tccataacct tgcagatctt ctcggcgtac acctcgccca
     3181 gagagccacc aaagacggta aagtcctgag agaagacgca gaccttgcgc cccgagacct
     3241 cgccgtagcc tgtgatgacc ccgtccccaa gcggcctgtt ctcctcgagc ccgaagttgt
     3301 aggaacggtg caccctgtag cggtcgagct cgacgaacgt gccctcgtcc agcagcagct
     3361 cgatgcgctc gcgggccgtc agcttgccgc gttcgcgctg cctctcgacc gccgcctcgg
     3421 gggcagggtg cgccgcggcc tccctcagct tctcgagccc ttcgatcttt ccggcggtgg
     3481 tttcctcgcc cagtctctca ccttgcatag aggggcattc tagcacgcag aaaaacggca
     3541 acttccaatg gaggaggaat agcctggcct accggatgcg aattcggttc tcttcgacta
     3601 tccagaggca gccttccaga ggttcaccgt cgatagctct caacaacctt tggaacacct
     3661 cgaggacgta cggcttatcc tgccggccga ggcgcagcac gatcagaccg ggataattct
     3721 ccggtggata ggttcttatg tcggcaaagt ccaggtccag ggttactaga actcgacctt
     3781 cggatctgca gacctcggac agcgcgtggt caccactgcc tacgagttgt tggtcatgta
     3841 tcgtcaccgc ctcgtggccg gctgcgagaa gctctccggc cacctctaca ggaaggttct
     3901 catcgatctt gaacttcaca gacagcgtta cgcgggcatg ctcacgaaac gctcacgtgc
     3961 cagatccgca gcgtaggcaa tggacgcctg tatggcctca cggctaatag aaggatagct
     4021 cctcataatc tcttccggcg tctgtcccac ggcaaggttg tcaaggatca cggacaccat
     4081 gaccctgcta cccttgacgc atggcttgcc gtggcacacc tcagggtcaa caataatgta
     4141 ctctcgccaa tccacagcca tctctcgtga ttttccgcgt acccttagtt taatgcgcca
     4201 ggccccggcc tgactaccat taatacccta acccatatgg aaggcccaaa tccacgccga
     4261 cgtcggtggc tgcgctagcc gatctctcac ctggcatagg ggggcattct agcacgcgca
     4321 aagtccccga ttctcgggcg cgaagcgagg ggtagagtag aatgtaatcc cggagaatct
     4381 accgggaatt ggaaaaggag atgcatatgc aggtcttcga gaagctggcg gagtaccgct
     4441 acgagcagct cgtgttctgc cacgacaagg cgacgggtct gcgcgccatc atcgccatcc
     4501 acgacaccac gctcggcccg gcattgggcg gctgccggat gtatccctac gccagcgagg
     4561 aagatgccat agtagacgta ctgcgcctgg cgcgggggat gacgtacaag gctgccgcga
     4621 gcggcctcaa cctcggtggc gggaagtccg tcatcatcgc cgatcccgat accgacaaat
     4681 cagaggcgct cttccgctcc ttcgggcgct acatcgagac ccttggcgga cgctacatcg
     4741 tcgccgagga cgtgggcacg tcgacggagg acgcgaacta catcagggtc gagacctcgc
     4801 acgtcgtcgg ggtcgacgtc acccgcggcg gctccggcga cccctcgcca ctcacggccc
     4861 tcggcgtcct ccaggggatg cgggcctgcg ccgaagacgt cttcggcacc acctcactcg
     4921 aaggccgggt cgtcgccgtg cagggcctcg gtcacgtcgg ctaccacctg tgccgcctgc
     4981 tcgacgatga cggggcgaac ctgatcgtga cggatgtgaa taaagcggcg gtcgagcgcg
     5041 tggtaacgga gttcggcgcc aaggcggtag agactgatga gatcctgacc atcccatgcg
     5101 acatcttcgc cccctgcgcc ctcggcgccg tcatcaacga cgacactctc cccaccctcg
     5161 actgccgcat cgtggcgggg agcgccaaca acgtgctcgc cgagtcgagt cacggcgagg
     5221 cgctggtgga acgcggcatc ctctacgccc ccgactacgt cataaacgcg ggcggcctca
     5281 tcaacgtcgc tgacgaactc gaaggataca acgaacgccg cgccaccaag cgcgtaatga
     5341 ggatcgaaga ccgtatacgc cgcatcatcg ccatctccaa acgcgacggc gtcccgacaa
     5401 acgtcgccgc cgacaccatg gcgctggagc ggatagccgc cataagctcg atggaacgcc
     5461 tccatacagg acacccctac gggcagttgg tccgccgccg tgagacgatt tagaggcatc
     5521 aggctacagg tttcagggtt cctgtaactg gctttcgtcg gtagtgcgtg acgtagcgag
     5581 ttttcgggag acagaagacc tcccgctcca cacgggaact ggctccacga tacctgatgc
     5641 ctgtagtctg aaacctgata cctttacgcc atccacgcga cgatcagcca ggcggtgacc
     5701 agggccagta ctagccagtc ggataggctc gcgcgcatgc ggtggagctt ggtgcggtaa
     5761 cggcctccgc ggtagctgcg ggtgttcatc gcgatggtga gggtgtcggc gcgggcgaag
     5821 ccgctgatga agatcgggac gagcaggcgg ccgaggcggc gcgcacgggt tatggcacct
     5881 ccctcgtcga atggtacgcc gcgggcggtc tgggctctgg cgaggcgctc catctcctcg
     5941 atgaagatgg ggacgaactt tatggcgatc actgagacaa gcaccagctc gttgacgggg
     6001 attctgagac gttgcagagg gccgaagatg agctctaggc cgttcgtcag gtcgacgaga
     6061 gaggtcgtca gcatcagcat ggtaatgacg tagtaaagga ggatgacgcg catcgcgatg
     6121 atgcctgccg tgtacagtcc ctccgtcgag accgagagga tcccccacct ccacaggtag
     6181 ccggattcgg gcacgtctcc tgggtagaag agcacctgga aggcgaggat gaagaccagg
     6241 aaaccgagaa agagccgcga gccccgcagc acgtaccccg gcggtatcct ggagacgatc
     6301 tgaatcagga cgaggagcgg cgtgacgatc gcgaagccga ggaagctatc tatgaggaac
     6361 gatgccacga tgaccaggaa cgtcgccgcg atcttcaccc tggggtccat gcgatgtatg
     6421 aacgagcccg tatcgacgaa ctgcccgaag atgacgttac gctggagttc taacactacg
     6481 ccgccccgag cctcttgagg agttcggact cgagctcttc ctccgtgagc agttccgtcg
     6541 ggaagtctgg gaagagcttc ctcatctcgc gcgcgccgac gacgggctca ggcagggtta
     6601 tgtcgagttc agcgagcctg tcggcctcgc tgagcacctc gcgtacggga ccgtccatca
     6661 cgaccgtgcc ccggtccaat acgatcaggc ggtcgcacag gaggctcgcg tcggtgaggc
     6721 ttgcggagca gacgatcagg gtgagttccc gctctctctg gagcttgatg aggagctcga
     6781 ggagctgcct gcgaccgcgt gggtcgagcc ccgccatcgg ttcgtcgaga acgagcacgg
     6841 gcgttcgcat ggcgaggacg ccggctatgg cgaccctcct gcgctgcccg ccggagagcg
     6901 cgtggacgta acgcaagcgg tagtcaggat aagggagccc catggtgttc aggctctcct
     6961 cgacgcgtgt cctggtctcg gaagccgaga ggcccgccgc ggtcggcccg aaggcgacgt
     7021 cggcgcctac aacgtcctct atgatctgcg agtcggggcg ctggaagacg atcccgacct
     7081 cacgccgcag cctgcccttg tcgtagccgc gagccgcgat gtcctcgcca ttgtagaaaa
     7141 cctgaccggg cctgacgcgc agcaaatgcg caaaggagtc gacgacggtg gacttgcctg
     7201 actgggtggc gcccactaca ccgacgcgct cgccgcgatg gatctggaag gatacgtccc
     7261 tgaccgcctc gctcgccctc ggggtgcctt cgaggtagac gtggctgaag ctcctcaact
     7321 ccagtaccgg agtcgtcccc tcagacatca tcagccgtat atcgaagggt caacggcggc
     7381 ggagagtgcg gcgcgaaggt cctccgtgtt caggataccg tccccgagag ggacgccgcg
     7441 ggcgcgcagt cggtgggcga ggctcgcggc gaggggtacg tcgagaccga cctcccgcag
     7501 ctcctcgacg cgcctgaaga cctccgcggg ggttccgtca aggaccacca gcccctcgtc
     7561 catcaccacg accctgtcga agaggacggc ctcgtacata gagtgggtga cgtgcaagac
     7621 gcccatgttc ccgctgcggt tcagttcctt tacggtcgag atcagccgca tagcaacggg
     7681 gacggggagg agcgaggtcg gttcgtccag gatgagaaag tggggtctca tcgccagggc
     7741 gcctgctatg gctaccctct gcttctgccc cggtgagagg tcctcgacga gtcgtcccct
     7801 tatctcctcc aggccgagcg cccgcaagga cgactcgacc cgctcaagga tctcctcgcg
     7861 agagaggcca aggttctcgg ggccgaacgc cacgtcgtcc tcgacgcggt tcatgacgag
     7921 ctggtcgtca gggtcctgga agacgaccga gaccttgcgt cgcacctccc actggtgctc
     7981 gtgatccacg ggcacgccgt ctatgcggat ctccccctcg gtcggatgga gcacggccgt
     8041 caacaacttg acgagcgtgc tcttccccga gccgttgtga ccgatgatcg cgacgagctg
     8101 ctccggctct attttcaggt cgagaccccg cagcgctgga acggcatcgg tcgagcccgt
     8161 ccggtacgta aacgagacgc cgtctatctc gatcatagaa tccggctagt agcggcgttc
     8221 ttcgtccgac tccgtctcgg gggccgtcgt ccgcccgctt cggaaaagca tcacgcccct
     8281 cacgataacg acagtgacaa cggcagcaag tacagcctcc gctatggcct gcggcaagac
     8341 ggcgggtatc acggcgggtg gcaacaatcc gaagaggatg gcgagaccga gtacccccac
     8401 cgtgttggtg aaggacccga gtagcccgga tacggctgct gccagatcca cactccagcg
     8461 cctcagccca acgaagacgg cccacgcgac aacccctatg aacagccgag gcacaatggc
     8521 cactatggga ttgaggaaga cgggattttc ggcttgtaga aacgagaata tgccgaagat
     8581 cgctcctgcc aacagcccca ctatgggtcc ttcgagtacc ccgccgacga tcgccggtat
     8641 gtgcattagc gtggcgttgc caatgagcgg tatcggcacc ggtatgaagc ccaggcgcgt
     8701 gaacccgagg aatagcgcga tgccgcctat tatgccggcc actactatct gcctcgtgtc
     8761 gaggctgaat atggagcccc gcccctggct tcccgaaccc gtcgacataa ctattcctcc
     8821 ttgtatatac cggcttctgg ccggactatg tgccgcgctc acctagtatc ggcatctgta
     8881 cgcatcctgt caaactacta gagtggcggt gtgaagcgtc cgtctgccgc ggtccagccg
     8941 ccgtcaacaa gcatgaatcc gccggtgacg taggagctgg cttcggaggc gaggtagacg
     9001 accatcccga ccatctcgtg cggcctggcc cagcgtccga ggatgctctt cgctgcgtag
     9061 gagtcatacc agtcggggga gtctttgatc tgggccgtca aaggcgtctc gacgatgcca
     9121 ggggcgatgg cgttgacacg gacgcctcgt ggtcctatct cggaggcaag ggcccgaagc
     9181 atctggaccg tacccgcctt cgtcgccgcg tagacaccct gcccgggctc gacgacctgc
     9241 ccgcgtatgc tcgagaaggc gatgatgctg cccgaaccac gttcggccat gccacgccca
     9301 aagtcacgga tcaggcggaa ggttcctttg aggttgagtt ccacgacttt gtcgaactct
     9361 tcgtccgtga tctccagcac gggtttcctg acgttgacgc tcggcgtact cacaagtacg
     9421 tcgggtgtgc ccacgcgctc gacggcggat cgtacgctct cagggtccgt catatccagt
     9481 tcgatggcat cggcctttcc ttccctcgac ctgatctctt gggccgtgtc gtctgcggcc
     9541 ccgccgttca cgtcggcgca taccacgcgt gcgccgaagg cggcgagccc gtgcgcgctg
     9601 gcctctccaa taccgctgcc tgctcctacg acgagcgcgg tcttatctgt caggtcgaac
     9661 atcgagccgt aatcaaacat gggcgtcctt tccatgggcg tagaacatcg ccgtcgctgc
     9721 gtagagcttg caggtcgtga ggagttcctc ctcgtctacc cactcgtcgg tgtggtgggg
     9781 gatctcacgg ttcccagccc ccgtcgtaac gatggggatg gcagcccacg cgtggaggaa
     9841 agtgccgtcg gtcgcgccag ggaccccgtt gtagcgcggc tcctcgccgg taaggtgggt
     9901 gtaggcttcc gccatcgccc ttaccagcgg ttcgtctttc ggcgtctcgg tcggggggcg
     9961 ctcttcgatc acctccagcg tcgcttcgaa gtcagggtct tctgaggcaa gcctcgcgag
    10021 gataccttcg agacgctcga tcaactcttc atgagactgg ctcgggacgg tgcggatatc
    10081 cagggcgacg taggcgctcg cggggatgac gttgatctgg ggttccccgt agtcggggcc
    10141 catcaggatc gtcggcgtca ggctcggata accgaggaaa gggtcctcgc cgtgacgttc
    10201 gacctcctcg ctttcgagct cctccacggc gacgacgaag cgcgcggccc gcgtcacagg
    10261 gttgacgccg cttcttggca tggccccgtg cgccatctgt ccacgcacgg tgacctccac
    10321 cctgagcgcg cccttctgcc gaatgcagag gtggttctcc tcgggctcgc agataactgc
    10381 ggcgtcaact ccttcggcgt ggccccgctc gataaacgcc ttgacaccgg tcatcatccc
    10441 ctcctcgtcc accggatgac agagtatcag cctgccgggg aaagggacac ctgaatccct
    10501 gatcgcacac acagccgtca cggcagccgc caggttccct ttcgtgtcgc aagccccgcg
    10561 cccgtagata cgtcctctat ctctctcggc cgcgaacggc tggtggtccc agtcctccgc
    10621 gtttccctcg gtaaccacgt ccgtgtgcgc ctcgaagagc agggtcctcc ccggactgtc
    10681 gccctcccaa acggcccaca cgttcggacg gcccggcgca actacgtcga cctgcacccc
    10741 gaagcccgcg cgctccagat aatccgcgac gaatccagca acgcgcgcct cgttgccttc
    10801 cggctcttca ggaaggtaga cgctcgggat gcgcacgagt tcttgtgcca ggcgtacgag
    10861 ttcctcgcgg tcgatacgac ccaatacgtc ttcgagcaca cgtcggaatg cggcgtttgc
    10921 tcccgcgtcg acgtcaggct ccgtcaactc aatcccgcca gcaccagccc atcgtcactg
    10981 ctcaagccgt cacctcaatg tatgccgagg aggtgctcgg cgaaggaaca gggaagcctt
    11041 cctcgcgcag tccctcgaga tggagttcga tagcctcctg cgtgttgtgc tcgacctctt
    11101 cacgagtcgc cccggtcgat acgcagcccg gtaggtccgg agagaaagcc gaaaaccccg
    11161 tttcggtggg ttctatgatc tccatgtgtc tcattgcgta ccctcgcgta tgcccatcct
    11221 tcactacgga aggaggtcac ggaccagcat cgtccatccg ggcaaggccg gttctgcttc
    11281 ggccacatcg tcactctgat aggtggccgg ccggtccggt tcgtcagcgc ggtaaacgtg
    11341 cacaacacgc tcgtgcagaa cgtcaacatc ccacaccacc agggtgccag cggcgaagta
    11401 gtcggtccgt ttttcagcca tctcctgttc ggttctctcg ccataatcgt gctcgctgcg
    11461 gacttccgcg gcgaagatcg gagcgccgtc caggaaccgt ccaccctgca agggaccggc
    11521 gtggaaggca gcgtcagggc tgaacgagcc gcgatgcggc aggttcaccg taaacccgac
    11581 gttgtcgggg aaagcatagc cgcgacccgt gcgccgctcg tagtcgcgca ggctgacgta
    11641 gatctcgccg cctgctcgac cggggatccc accagtcggc gccataagca ctatctcccc
    11701 atttacaagc tccgccttgc cggtctccgg caccctgtac agatcttcaa tcgtcgctct
    11761 cgtgcgtgga ctcatgagat tccacctgac ctctccgctc cccgccatta tatcgaccga
    11821 gcgggaccag cgagtcgcga ccagctccga gttaaagcct caagcgcttc ggcttgcgac
    11881 ttatcgctca tcgtaagctt tcccttcgaa atttgtccta ctgctcacag catgacgtcc
    11941 cgaaaaaacg tactgcggcg tctgatacgt caatcaatct gcggtcaacc gataccgctc
    12001 tccggtccgt aatcttccaa cctccgcttg acggcattga ggaacttgag cgcggcgccg
    12061 ccgtcgagga tgcggtggtc gaaggagacc tcgaggttca tcatgctgcg gatggtgatg
    12121 gcgtcagtac cgccgtcgtc tatgacgact ggacgtttga cgaccgcctc agccgaaagt
    12181 atcgccgcct gcggatggtt gattatgggc gtcgagacca cgctaccaag agagcccgga
    12241 ttgtttacgg tgaaggtgcc gcccgagatg tcttcggtgc cgagttgacg gtcgcgggca
    12301 cggcgcacaa gatcgtcgat cctgcgtgcg agcccgagga tattcagctc gtcggcgtcc
    12361 gggatgacgg ggactatgag ggcgtcttcg agatcgacgg cgacccctac gtttatgcgc
    12421 tttctcagga cgatacggtc ctcgtcccac acggagttga gaaccggatt ttccttgagg
    12481 ctctcgacta ctgctttgac gatgaacggc aggtaagaga gcttcatacc ttc
//
LOCUS       DATAIR010000434         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_197247, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000434 DATAIR010000000
VERSION     DATAIR010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197247"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VHM69_10555"
     CDS             <1..1228
                     /locus_tag="VHM69_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX2740879.1"
                     /translation="QQTQAISQALTLAHPMVDVHARYIHTLEHGGRLNRELEFLPGDE
                     ELGERRSDGKGLTAPELAILLPYSKITTYKDLLNSDAPEDPYLSRELERYFPTPSRAR
                     FREQIHEHRLHREITATHVTNSLVNRCGPSFGFRIGEETGAEAPDIARAYTAAREIFS
                     MRSLWDGIEALDNRVEARLQTRIMLDARKLVERATRWLLRNRRAPLDIGATISHFSEG
                     AAELSELIPGILLDGDREAVENAAQRLIDANVPPDLAWRAARLGPMFSSLDITDVANA
                     TGESLDTTAAVYFTLGDRLKLHWLRRHIEALPRDNRWRTLARSALRDDIFNQQAALAA
                     EVLRNTPEERDAHERIEAWVEANEGPVDRTLQVLADINASGTFDLSTLSVALREIRNL
                     VTAPEASPQEVEATAR"
     gene            complement(1380..1979)
                     /locus_tag="VHM69_10560"
     CDS             complement(1380..1979)
                     /locus_tag="VHM69_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740880.1"
                     /translation="MKRLTLLLTMMALALLLSASVAFALTVDCTAGRGCFGTDDPDTL
                     NGSAGSDEMDARQANDRLFGGDGHDWMSGDAYGPTDTSTDGDDRVIGEAGSDGIVGYG
                     GGDVLSGGDGRDYLFAVENSKNPGEDTVRGGPGNDFIDAIDKTKDTIDCGTGTRDRVY
                     YDKNLDTVERCEIERTQYPGDEFLATLAVDEEANTSRAR"
BASE COUNT          461 a          796 c          650 g          433 t
ORIGIN      
        1 ccagcagacg caggcgatca gccaggccct caccctggca caccccatgg tcgacgtcca
       61 cgcccgttac atccacaccc tcgaacatgg tggcaggctg aaccgcgaac tggagttctt
      121 gcctggtgac gaggagcttg gcgaacggcg ctctgacggc aagggcctca ccgctcctga
      181 gctcgccatc ctgttgccgt acagcaagat cacgacctac aaggacctcc tgaactcgga
      241 cgcccccgag gacccgtacc tttcacgcga gttggaacgt tacttcccga caccctcgag
      301 agcacggttc cgagagcaga tccacgagca tcgtctgcac cgcgagatca cggccaccca
      361 cgtcacgaac agcctggtga accgctgcgg cccttccttc ggcttccgta tcggggagga
      421 gacaggggca gaggcgccag acatcgcacg cgcctacacg gcggcgcgcg agatcttctc
      481 catgcgcagc ctgtgggacg gtatcgaagc cctggacaat agggtcgagg ccaggctgca
      541 gacccggata atgctcgacg cccgcaagct cgtcgagcgc gcgacccgct ggctgctccg
      601 caaccgccgc gctccgctgg acataggcgc gacgatctcg cacttctccg aaggggccgc
      661 ggagctctcg gaactcatcc ccggcatcct gctcgacggc gaccgcgagg ccgtcgagaa
      721 cgcggcgcag cgcctgatcg acgctaacgt accgccggac ctggcctggc gtgcggcacg
      781 cctcggcccg atgttctcct ccctggacat taccgatgtg gcgaacgcca ccggagagtc
      841 cctggatacg accgctgcgg tctatttcac ccttggcgac cgcctgaagc tccactggct
      901 gcgccgccac atcgaggctc tcccccgcga caaccgctgg cgtaccctgg cgcgctccgc
      961 gctacgcgac gacatcttca atcagcaagc cgcccttgcc gcggaggtgc tgaggaatac
     1021 acccgaagag agagacgccc acgaacgaat agaggcatgg gtcgaagcca acgagggacc
     1081 tgtggatcgt accctgcaag tcctcgcgga cataaacgcc agcggcacct tcgacctctc
     1141 cactctctcg gtcgcgctgc gtgagatccg gaacctcgta accgccccgg aagcctcacc
     1201 gcaggaggtg gaagcgactg ccagatagag gcaacaggct aaagcggata gcagaaggat
     1261 ccttctgcta tccgctccgg tcagcttttt ctcgactcac cgcgggatca cgtatttcga
     1321 gaaacactgt cacttcagcg cctgcgaggg tcgcgattac gactgatcga accctcgcgc
     1381 taacgcgctc ttgaagtatt ggcctcctcg tccacggcta aggtcgcaag gaactcatct
     1441 ccgggatact gcgtcctctc gatctcgcac ctttcgaccg tgtcgaggtt cttgtcgtag
     1501 tagaccctat cccttgtgcc agtgccgcag tcgatcgtgt ctttggtctt atctatcgcg
     1561 tcgatgaaat cgttgcccgg accgcctctg acggtgtcct cacccggatt cttcgagttc
     1621 tccacggcga acaagtagtc gcgtccgtct ccaccagaga gtacgtcgcc gccgccatag
     1681 ccaactatcc catcggaacc agcctcgccg ataacgcggt cgtcgccgtc cgtggaggtg
     1741 tcggtcggcc cgtaagcgtc gcccgacatc cagtcgtggc cgtctccacc gaagagcctg
     1801 tcgttggcct gcctggcgtc catttcgtcg ctaccggcag aaccgttgag cgtgtcaggg
     1861 tcgtcggtgc caaagcagcc ccgaccagca gtgcaatcga ccgtcaatgc gaaggccacg
     1921 ctggcactca gcagcaacgc caacgccatc atcgtgagta gtaaagtaag ccgcttcatc
     1981 tccgtttcct tgcctttcgt agttggtact tcctcccgca ctgcatccgt cgatccaaac
     2041 cctcacctcc tttccggttt cgcacccgcc atcaaatctc cgccataaac cgtcacccgt
     2101 gcgccccatc tctacttcta tacttacaac gtaaacttac gtcgtaaatc tacaccaatg
     2161 cgactaatgt tgtcaagggt cattagccaa attgcctgaa gacgttgttg aggctcccgc
     2221 aggcaggcta atgacgagca ggcggagagc agattggtga cgctggtggg acgccgaggc
     2281 gcacgggagc tagttgtact ggcggagagg tctaacgatg tgacgcacga tggagccgcg
//
LOCUS       DATAIR010000435         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_304857, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000435 DATAIR010000000
VERSION     DATAIR010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304857"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VHM69_10565"
     CDS             <1..168
                     /locus_tag="VHM69_10565"
                     /inference="COORDINATES: protein motif:HMM:NF022060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2740881.1"
                     /translation="EADYGGLEVPRPPFWGGYRVEPEVIEFWQGRENRLHDRLVYRRT
                     KGEWRIERLQP"
     gene            230..1048
                     /locus_tag="VHM69_10570"
     CDS             230..1048
                     /locus_tag="VHM69_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2740882.1"
                     /translation="MKLVTYSVDGGETRIGSIDNGEIQPLGGDSMVEFIEHGGSPEPG
                     ESTVSFDEARIHAPIERPQKLICIGLNYEDHAAETGADIPEKPIVFAKFPNTVIGHGE
                     AIRIPPITEQADYEAELAVVIGRAARNVSVSEALDYVFGYANANDVSSRDLQFSEGGQ
                     WTRSKSIDTFCPIGPYIATTDEIEDPQNLWIRCILNGEVMQDGTTSKMIFSVAELVSF
                     LSEGMTLVPGDVIITGTPPGVGMARDPQVWLKAGDEVSIEIEGLGTLTNPVETA"
     gene            1052..2422
                     /locus_tag="VHM69_10575"
     CDS             1052..2422
                     /locus_tag="VHM69_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2740883.1"
                     /translation="MDLLAELGTVLSEDRIVLSGEDLERHGGGVFTYHAPISPDAVVY
                     PQSREEVVEVLRFANEHRVPIVPFGQGSSLEAHTLPVRGGISLDLARMDRILEVRPDD
                     FIARVQPGVTHEALNADLAGHGLLFSVDPGWDASLGGMAGTNASGTNAVKYGVMRDQV
                     LGLEVVLGDGTVMRTGGMAMKSSAGYDLTGLFVGSEGTLGVFTELILRLYPIPEHTVA
                     ARAVFSDIEAAGRAAVAMIRTGMRIGRVELVDARTVGAVNTYKGTDYAFAPTLFLEFS
                     GSEASVKDDVEVAREISDSEGCSSFEFEADEAAREKLWEARHDAALAIRDLYPGKGMM
                     GTDVCVPISDLPGALRHARDAIASRGIDGAILGHVGDGNYHAVFPVDTEDRADQERAE
                     AVNAEIVDYALERGGTCTGEHGIGMGKTGHLQKEHGDSLPFMREIKRIADPNDIMNPG
                     KIFPGE"
     gene            complement(2561..2995)
                     /locus_tag="VHM69_10580"
     CDS             complement(2561..2995)
                     /locus_tag="VHM69_10580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2740884.1"
                     /translation="MRVEESIEINSPLEEVFDYVSDVGNFPEWTAHTLEVRKDTLGPP
                     QESDRFTVAIKSLGRRFETPYERTSFEPGRRYTDRAVGGPIPNQQWDYTFQEVPGGTR
                     LTRAVEATPGGFLKLLEPLQKRAVKRQLATDMETLKELLEAR"
     gene            3228..3710
                     /locus_tag="VHM69_10585"
     CDS             3228..3710
                     /locus_tag="VHM69_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740885.1"
                     /translation="MKTWMKVSLITAVVGIPAFFLGPALFPPAAGGPSPTAGQLPYFL
                     FLAVGDALLLGLGVSFLLFGFPVMRKVSPDSRLRAWTMYLSIGYLMVSWWPHLNLHAH
                     NGLNLQGLLYIDYFFHLPLEIAGVALALCFISLMRSRTDEAPASEILAPAGEASASAE
                     "
     gene            3772..3990
                     /locus_tag="VHM69_10590"
     CDS             3772..3990
                     /locus_tag="VHM69_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740886.1"
                     /translation="MKIRWKVLLTATVALALNLSLASAALAHGGVPDGHSEQASGLDW
                     SSLLVPPEFVLGGVALVGALYAVKVLRG"
     gene            4176..4610
                     /locus_tag="VHM69_10595"
     CDS             4176..4610
                     /locus_tag="VHM69_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740887.1"
                     /translation="MVDPDKTSDRPVRAIGVLLILEVIVIVALGGYEFFQLDWRAIDL
                     DDPSQREIEAAVFALLVPSAVLTLMSAMSFLFLRRRGWLMAAIAQGLSLAVCLWLYTL
                     FQPDYVYPIMAFCVLMILYLNSHEVRVVFHPRRSAARHGKTL"
     gene            4607..>5250
                     /locus_tag="VHM69_10600"
     CDS             4607..>5250
                     /locus_tag="VHM69_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740888.1"
                     /translation="MKAGAEAATALLRRFEPVLRSTRGDKFYPMDVEPYVRACSLWVQ
                     RPGEAAVCVVRGGKLDLAELAQQPLDESGAVHFLKFTDEDDIGHDSHGRRLKLFRRRA
                     KDRRESTDAFRAGRGRLARVGYVSRFVDALYSIALLARGRVPGESAAAASVAYERIMA
                     EQEHYRYHGRVLRQDGWIVLQYWLFYPFNDWRSGFFGANDHEADWEKVFVYLSES"
BASE COUNT          980 a         1605 c         1676 g          989 t
ORIGIN      
        1 gaggcggatt acggggggct tgaggtcccg cgcccaccgt tctggggagg ctacagggtc
       61 gagcccgagg tcatcgagtt ctggcaggga cgcgagaaca ggctgcacga ccgtctcgtc
      121 taccgtcgta ccaagggaga atggaggata gagcgtctgc aaccgtaggg acgctggtgg
      181 ccccgccttg ctgctaagat gcgttctgga acagatggag aggagaacct tgaagctggt
      241 aacgtattct gtggatggtg gagagaccag gataggctct atcgataatg gagagataca
      301 gccacttggc ggcgacagca tggtcgagtt tatcgagcat ggtggaagcc ccgagcccgg
      361 cgagagcaca gtatcgttcg acgaggcgcg catccacgcc ccgatagaga ggccgcagaa
      421 gcttatctgc atcggcctca actacgagga ccacgcggcc gagacaggcg ccgacatccc
      481 cgagaaaccc atcgtattcg ccaaattccc gaacacggtg atcgggcacg gagaggccat
      541 caggataccc cccataaccg agcaggcgga ctacgaggct gagctcgctg tggtcatcgg
      601 tcgggccgcg cgcaacgtct cggtctctga agccctcgac tacgtcttcg gctacgcgaa
      661 cgccaacgac gtctcctcgc gagacctgca gttctcggag ggaggacagt ggacgcggag
      721 caagtccata gacaccttct gtcccatcgg cccgtacatc gcgaccacgg acgagatcga
      781 agacccgcag aacctctgga tccggtgcat cctgaacggc gaggtcatgc aggacgggac
      841 gacgtcgaag atgatcttct cggtcgcgga gctggtctcc ttcctctccg agggcatgac
      901 cctggtccct ggcgacgtca taatcacggg tacgcctccg ggcgtcggca tggcccgtga
      961 cccgcaggta tggctgaagg ccggggacga ggtgagcatc gagatagagg ggctcgggac
     1021 cctcaccaac ccggtcgaga cggcatagat catggacctg ctcgcggaac tcggcaccgt
     1081 cctgtcggag gaccgcatcg tcttgagtgg cgaggacctg gagcgccacg gaggcggtgt
     1141 cttcacctat cacgccccga tctcgcctga cgccgtggtc tacccgcaga gtcgcgagga
     1201 ggtggtggag gtcctgcgct tcgccaacga gcacagggtg cccatcgtgc ctttcggaca
     1261 ggggagcagc ctggaggcgc acaccctgcc tgtccgaggc ggcatcagcc tcgacctggc
     1321 gcggatggac cgcatcctgg aggtcaggcc ggacgacttc atcgcgcggg ttcagccagg
     1381 cgttacccac gaagctctca acgcggatct cgccggacac gggctgctct tctcggtgga
     1441 tcccggctgg gacgcgtcgc tcggcgggat ggcggggacc aacgcgagcg ggacgaacgc
     1501 cgtcaagtac ggcgtgatgc gggaccaggt cctcggcctc gaagtggtgc tcggcgacgg
     1561 gacggtaatg cggaccggcg ggatggcgat gaagtcatcg gcaggatacg acctgaccgg
     1621 tctgttcgtc gggtcagaag gaacactcgg cgttttcacg gagttgatcc tgcgccttta
     1681 cccgatacct gagcatacag ttgcagcaag ggccgtcttc tccgacatcg aggcggctgg
     1741 aagggccgcg gtggccatga tccgtaccgg catgaggata ggccgggtcg agcttgtgga
     1801 cgcccgcact gtcggggccg tgaacaccta caagggcacc gactacgcct tcgccccgac
     1861 actcttcctc gagttcagcg gctcggaagc gagcgtgaaa gacgacgtcg aagtcgcgcg
     1921 cgagatctct gactccgaag ggtgctcgtc gttcgagttc gaggccgacg aggctgcgcg
     1981 cgagaagctc tgggaggcac gccacgacgc ggccctcgcc atccgcgacc tctatcccgg
     2041 aaagggaatg atgggcacgg acgtgtgtgt cccgatctcc gaccttcccg gcgcccttcg
     2101 tcacgcccgc gacgccatcg cctcgcgcgg catcgacggg gccatcctcg gccacgtcgg
     2161 agacgggaac taccacgccg tcttccccgt ggacacagaa gatcgggccg accaggagcg
     2221 ggccgaggcc gtcaacgccg agatcgtgga ctacgccctg gagcgcggcg gcacctgcac
     2281 cggcgagcac ggcatcggta tgggaaagac gggccacctt cagaaagaac acggcgactc
     2341 cctgcccttc atgcgcgaga taaagaggat cgccgatcca aacgacatca tgaaccctgg
     2401 caagatcttc cccggcgaat agcgtcaggc gccccgagtg ccgcgctggt tgagcacacg
     2461 ttcgttccct gatgtcgtaa ccgccacatg gcgatcgtgg gagcacgccg agaagcatta
     2521 gagcgtagga tatccccccg gttcgctccg ctcttcgtcg ctaccgcgcc tccagcagtt
     2581 ccttgagcgt ctccatgtcc gttgcaagtt gtcgctttac tgcccgcttc tgaagcggtt
     2641 ccaacagctt caggaagcca cccggcgttg cctccacggc tcgcgtcaag cgtgtccctc
     2701 ccggtacctc ctggaaggtg tagtcccact gctggtttgg gatcgggccg cctacggctc
     2761 gatctgtata ccgccggcca ggctcaaagg aggtcctctc gtagggcgtt tcgaagcggc
     2821 ggccaaggga ctttatggca acggtgaacc gatcactttc ctgcggtggg ccgagtgtgt
     2881 ctttgcgtac ttctaaggta tgggccgtcc attccgggaa gttgcccacg tcggagacat
     2941 agtcgaagac ctcctccagg ggactattga tctcgatgct ttcttcgacg cgcataatgg
     3001 ccaagtgctc cgtctcaacc cgcttccgat accatgatgc taccagataa agcctgatgc
     3061 gccgcttcga acgctattcc tggaaatgac ttctacgaag ttaggaacga ctcgaagcta
     3121 gcccacatgc ggtactcaaa aatcatcctt ttgcggtatg cgccggggcc gcgccaccca
     3181 gcataatttc ccacggaata cttcggagca gaaaggagaa gaatactttg aagacctgga
     3241 tgaaggtttc gctgataacc gcggtggtgg gcatccctgc tttcttcctg ggaccagccc
     3301 tcttcccacc cgccgcggga ggaccgtcgc ccaccgccgg tcagttgccg tacttcctct
     3361 tcctcgcggt tggcgacgcc ttgctgctgg gccttggggt ctccttcctg ttgttcgggt
     3421 tccccgtcat gcgcaaggtc tcccccgact ccaggctgag ggcgtggacg atgtatctct
     3481 ccatcggcta tctgatggtc tcctggtggc cgcaccttaa tttgcacgcc cacaacggtc
     3541 tgaacctgca ggggctgctg tacatcgact acttcttcca cctgccgctg gagattgctg
     3601 gcgtggcact ggcactgtgc tttatctccc tgatgaggag ccgtaccgac gaagcacccg
     3661 ctagcgaaat actggctcct gccggtgagg catccgcctc agccgagtag gacaccaatg
     3721 agggccgcag ggggcatgct cccgacgccg tcttcagaga aggagactga ggtgaagatc
     3781 aggtggaaag tcctgttgac ggcaaccgtt gcgctcgcgt tgaacctgtc gcttgcgtct
     3841 gccgccctcg cccatggtgg cgtgccagac ggccactccg aacaggcgtc tggccttgat
     3901 tggagcagct tgctggtgcc gcccgagttc gtgctcggcg gcgtcgccct cgtcggcgct
     3961 ctctacgcag tgaaagtgct cagaggatga gcggcttgta ggagagtgga gtttcaggag
     4021 tcgccaccag gaaatcggtc gacgagacct gtctagaagg ccagggcatc tcggtgcgcg
     4081 gtcctactcg ccgtgacggg tttcggcgtg taatctgttg ccaacatcac ggtagtggcg
     4141 aattatagac ttgaggagga ggaagcacat aaggcgtggt ggatccggat aagacatcag
     4201 accgccccgt gcgggcgata ggcgtgctct tgatcctgga ggtcatagtg atcgtggccc
     4261 tcggcggcta cgagttcttt cagctcgact ggcgggcgat agatctcgat gatccttcgc
     4321 agcgagagat agaagcggcg gtcttcgctc tcctggtgcc gtccgcggtc ctgacgctga
     4381 tgtcggcgat gagcttcctc ttcctgcgcc gcaggggatg gctaatggcg gcgatcgccc
     4441 aggggttgag cctcgcggtg tgcctgtggc tctatactct cttccaacca gactacgtct
     4501 acccgatcat ggccttctgc gtactgatga tcctctacct caactcccac gaggtgcgcg
     4561 ttgttttcca cccgaggcga agtgccgcta gacacgggaa gaccttatga aggccggcgc
     4621 agaagccgcg acggccctgc tgcggcgctt cgagcccgtg ctccgctcga cgaggggcga
     4681 caagttctat cccatggacg tcgagcccta cgtgcgggcc tgtagcctct gggtgcagag
     4741 gccgggcgaa gcggcagtct gtgtagtgcg tggcggaaag ttggacctgg ccgagctcgc
     4801 gcagcagccc cttgacgagt ccggggcagt tcacttcctc aagttcacgg atgaagacga
     4861 tatcgggcac gactcgcacg gcaggcgcct aaagctgttt cgcagacgtg cgaaggatcg
     4921 cagagaatcg accgacgctt tcagggcggg gcgcgggcgg ctagcgcgcg tcgggtacgt
     4981 ctcccgcttc gtggacgcgc tgtactcgat agcgctcctc gcccgcggaa gggtgccggg
     5041 ggaatcggcc gcagcggcgt cagtagccta cgagcgcata atggccgagc aagaacacta
     5101 ccggtaccac gggcgcgtac tgcgccagga cggttggatc gtcttgcagt actggctttt
     5161 ctaccctttc aacgactgga ggagcgggtt cttcggcgcc aacgaccacg aggcagactg
     5221 ggagaaggtc ttcgtctatc tctcagaatc
//
LOCUS       DATAIR010000436         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_27729, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000436 DATAIR010000000
VERSION     DATAIR010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27729"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..540)
                     /locus_tag="VHM69_10605"
     CDS             complement(31..540)
                     /locus_tag="VHM69_10605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX2740889.1"
                     /translation="MPQVGQGILKGMGVTLQHLFGQKITRQYPEYKRQLPERSRGMLT
                     VDMDRCIACLQCMRICPDHCISIEQEKRDADGSGKAKPYAVGFVIDDSRCMYCSLCVE
                     VCPVNCIYHTEEFEAQAYNRLELARQFGERPVDPTIDPKPTVKKKKKPTREKAPRTVE
                     RPRTGDEAA"
     gene            complement(550..1551)
                     /gene="nuoH"
                     /locus_tag="VHM69_10610"
     CDS             complement(550..1551)
                     /gene="nuoH"
                     /locus_tag="VHM69_10610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2740890.1"
                     /translation="MQTVLNLLEVEPIRLLISFGAVLFLVLNLAAVLTMAERKVSGYI
                     HLRYGPNRVGPRGLLQPAADVFKLFSKENVSPGRSDIWVFLGAPIAMFIPAALVWIVV
                     PFAPDAVVADLNVAILFFVAITSIGALGVVMAGYGSRSAFSLLGALRGAAQMISYEVP
                     LILSLLGVVMLTGSLSLVDVVKAQGDTIWTWYFLPQLPMFITFYIAGIAEVKRVPFDL
                     PEGESEIVGGFMVEYSGMTWALIQAAEFASMAVMSAVTVTLFLGGWQPPLPFLDLGAF
                     NWVWFGIKTALIMFTFQWIRWSVPRLRMDQLMDLGWKVLVPITLLWLFVTAGAMLIF"
     gene            complement(1552..>2711)
                     /locus_tag="VHM69_10615"
     CDS             complement(1552..>2711)
                     /locus_tag="VHM69_10615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX2740891.1"
                     /translation="SAALSGRASGRLANPDVRDEFGLETLDMNMGPQHPAMHGLLRLI
                     LELDGETVVRCDPVMGYLHRCQEKIAENRMYPAVIPLTDRLDYFANMHNEHGYCLAVE
                     DLLDVEIPPRAEYIRVLMCELMRLASHIPSIGFLMLELGAFTPILYAFRERERIQTIF
                     EAVTGARMMFHYIRIGGVKADLPPEIPQKIWEYIESLEAHFQRDFVDLIAGNEIFISR
                     TRNVGNMTQEQAVEMGVTGPALRCTGVDFDIRRDYPYSVYPELEFDVITDTAGDVEAS
                     YRVRIAECLQSIRLIKQSLEKLPEGEVMGDTGRRIRPAEGEGFGRVESPRGEYAVHVV
                     SDGSDTPYRVHYRDPSFCNMQLLQEIVPGHYLPDIMPIMGLVDPVSGGWDK"
BASE COUNT          534 a          867 c          798 g          512 t
ORIGIN      
        1 tcatccctgc caggaaagcg aacgcgaccg tcaagccgct tcgtctccgg tcctgggacg
       61 ctctacggtc ctgggagcct tttcccttgt gggctttttc ttcttcttga cggtgggctt
      121 cgggtctatg gtcgggtcga cgggacgctc accgaactgc ctggccagct ccaggcggtt
      181 atacgcctgc gcctcgaact cttctgtgtg gtagatgcag ttgacggggc agacctcgac
      241 gcatagggag cagtacatac agcgggagtc gtcgatcacg aacccgaccg cgtaaggctt
      301 ggccttgcct gacccgtcgg cgtcccgctt ctcctgctcg atcgagatac agtggtcggg
      361 gcagatcctc atgcactgca ggcacgcgat acaacggtcc atgtccacag tgagcatccc
      421 gcgactacgc tcgggcagct ggcgtttgta ttccgggtac tggcgggtga tcttctgccc
      481 gaagaggtgc tgtagcgtca cccccatccc cttcaaaatg ccctgtccta cctgcggcat
      541 caaaacctcc tagaaaatca gcatcgctcc ggccgtgacg aacagccaga gtaaggtaat
      601 gggcacgagg accttccaac cgaggtccat cagctggtcc atccttaggc gcggcacgct
      661 ccaccggatc cactggaagg tgaacatgat gagcgcggtc ttgatcccga accacaccca
      721 gttgaaggct ccgagatcca gaaacggcag cggaggctgc cagcctccga ggaaaagcgt
      781 cacggtgacc gccgacatga ccgccatcga ggcgaactcg gcggcctgaa tgagcgccca
      841 cgtcatcccg gagtattcga ccatgaagcc gccgacgatc tcgctttccc cttccggcag
      901 gtcgaacggg acccgcttta cctcggcgat cccggcgatg tagaaggtaa tgaacatcgg
      961 gagctgaggc aggaagtacc aggtccagat cgtatccccc tgcgccttca cgacgtcgac
     1021 gagcgagagg ctccccgtga gcatcacgac gccgaggagg ctcaggatca ggggcacctc
     1081 gtaggagatc atctgcgccg ccccgcgcag cgccccgagc agcgagaacg ccgagcggct
     1141 cccgtagcca gccattacga cgcccaaagc tccgatagac gttatagcca cgaagaacaa
     1201 tatagccacg ttcaaatccg cgaccacagc gtccggcgcg aacgggacca ctatccagac
     1261 cagcgccgcc ggtatgaaca tggctatggg cgccccgagg aagacccaga tgtccgacct
     1321 tcctggcgag acgttctcct tggagaacag cttgaagacg tccgccgcag gctgcaacaa
     1381 cccgcgcggt ccgaccctgt tgggtccgta gcggaggtgg atgtagccag acaccttccg
     1441 ctccgccatc gtcaggactg ccgcgaggtt cagcaccaaa aacaggacgg ctccgaagga
     1501 gatgagaagc cgtatcggtt cgacctcgag aaggttgagg accgtctgca tctacttgtc
     1561 ccacccgccg gatacgggat ctacgagacc catgatcggc atgatgtcgg gcaggtaatg
     1621 tcctggaacg atctcctgta gaagctgcat gttgcagaag ctcgggtcgc ggtagtgcac
     1681 cctgtacggc gtgtccgagc cgtccgagac tacgtggact gcatactccc cacgcgggct
     1741 ctcgaccctc ccgaatccct cgccctcggc aggcctgatg cggcggcccg tgtcccccat
     1801 gacctcgccc tcaggcaact tctcgagact ctgctttatg agcctgatgc tctgcaagca
     1861 ctcagcgatc ctgaccctgt acgaagcctc gacatcgccc gccgtatcgg taattacgtc
     1921 gaactccaat tcgggataga cactgtacgg gtagtcgcgg cgtatatcga agtccacgcc
     1981 ggtgcagcgc agagccggtc ccgtcacgcc catctcgacg gcctgctcct gcgtcatgtt
     2041 gccgacgttg cgcgtacggg agataaagat ctcgttgccc gcgatcaggt caacgaagtc
     2101 cctctggaag tgagcctcga ggctctcgat gtactcccag atcttctgcg gtatctctgg
     2161 cggcaggtcg gccttgacgc cgcctatcct gatgtagtgg aacatcatcc gcgcgcccgt
     2221 gacggcctcg aagatggtct gtatgcgctc cctttcgcgg aaagcgtaga ggataggagt
     2281 aaatgccccg agctccagca tgaggaatcc gatagatggg atgtggctgg cgagccgcat
     2341 cagctcgcac atgaggactc tgatgtactc ggctcgcggc ggaatctcca cgtcgagcag
     2401 gtcctcgacg gcgaggcagt agccgtgttc gttgtgcatg ttggcgaagt aatcgagcct
     2461 gtcggtcagc gggatgaccg ccggatacat gcggttctcg gcgatcttct cctggcatcg
     2521 gtgcaggtat cccatcacgg gatcgcacct gaccacggtc tccccatcaa gctcgaggat
     2581 gagcctgagc agcccgtgca tcgcggggtg ctgcggcccc atgttcatgt ctagagtttc
     2641 gagcccgaac tcgtcccgga cgtccgggtt agcgagccga ccgctcgccc ggccgcttaa
     2701 agctgcgctc t
//
LOCUS       DATAIR010000437         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_120923, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000437 DATAIR010000000
VERSION     DATAIR010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120923"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VHM69_10620"
     CDS             <1..221
                     /locus_tag="VHM69_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740892.1"
                     /translation="PAPRARRTAERRRPRTLIEEARFRPAPSTAWARMIFDSLGSASS
                     TVTLYTPGAGGEDRYVRRFGADGWGIGP"
     gene            231..>1800
                     /locus_tag="VHM69_10625"
     CDS             231..>1800
                     /locus_tag="VHM69_10625"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740893.1"
                     /translation="MLGRYAELKAQLPPGTILFYQVGTFFETFEEDAKTISQALSIRL
                     TSREAAGEGRVPLAGVPGHAIQEHLATLLRKGYSVAVAEQRQHPTKPKQFTREVTRVL
                     TPGTVIEDNVLSAGHANYLAAFVVRDGRAGIAVVEASTGEFTGTEVPEEQLPAELERW
                     SPREIVVPERTAAENLPKVRATVSSAPRWTFEPSAGQQALKRHFRVSGLKGYGLDGRS
                     ALAGAAGALIQYLASLRGGNPPEQVVTFRPYDPGSGMVLDAATRRNLGLDDLVETIDR
                     TSTPMGQRALRRWLERPLLEVDRIKQRLGAVDALAGDYMLREEVREHLGSIPDVERIA
                     TRIVRLSASPNDLLSLKGALQEIGPLGETLEPASSRSSLLSRVLGAMEEPPGVRELIA
                     AAISEESGEIIRPGYSEELDEAREFRDGAHAWLTRFEAEERLKTGLKTLKVGYRDGEG
                     YFIEVAGKETSAVPERYQHRKALKHNSRYVTVELKEHESRMLGARDEVERIERRILSE
                     IRAAIKEAAPELQKI"
BASE COUNT          319 a          577 c          629 g          275 t
ORIGIN      
        1 atccggcgcc gagagcgagg aggacggccg agcgccggag gccgcggacc ctgatcgaag
       61 aggcgaggtt caggccggcg ccgagtacgg cgtgggccag gatgatcttc gatagccttg
      121 ggtctgcatc ttccacggtg acactgtaca cgccgggtgc cggcggcgag gatcgctacg
      181 tccggcgttt cggggctgac gggtggggga tcgggccgta gaatagcctt atgctgggac
      241 gttacgcgga gctgaaggcg caactgccgc cagggactat cctcttctat caggtcggga
      301 cctttttcga gaccttcgag gaggacgcga agacgatctc gcaggcgctc tcgatccggc
      361 tgacgagccg cgaggcggcg ggtgagggcc gcgtcccgct cgccggggtg cccggccacg
      421 ccatccagga gcacctggct actctcctca ggaaggggta ctctgtcgcc gtcgccgagc
      481 aacggcaaca tccgacgaaa ccaaagcagt tcacgcgtga ggtcacccgt gtcctgacgc
      541 ctggtaccgt catagaggac aacgtcctat cggcaggaca cgcaaactac ctggccgcgt
      601 tcgtcgtaag ggacggccgg gctggaatag cggtcgtcga ggcttcgacg ggcgagttca
      661 ctggcaccga agtccccgag gagcagctcc ccgccgagct ggagcggtgg tcgccgcgcg
      721 agatcgtcgt ccccgagcgg accgcggccg agaacctgcc gaaggtgcgc gccaccgtaa
      781 gctccgcccc gcgctggact ttcgagcctt ccgccgggca gcaggccctg aagaggcact
      841 tcagggtctc ggggctcaag ggatacgggc tcgatgggcg ctccgcgctc gccggagccg
      901 cgggggcttt gatccagtac ctcgcctccc tgcgcggcgg caacccgccc gagcaggtcg
      961 taaccttccg tccttacgat ccaggctccg gaatggtcct cgatgcggcg acgaggcgca
     1021 acctgggact agatgacctt gtggagacca tagaccgtac cagcacgccg atgggccagc
     1081 gcgccttgcg gcgctggctg gagcgtcccc tgctcgaagt cgaccgtatc aaacagcgtc
     1141 tcggggccgt ggacgccctc gcaggggact acatgttgcg tgaagaggtg cgcgaacacc
     1201 tcggcagcat ccccgacgtc gagcgcatcg ccacgcgcat cgtgcgtctt tcagcgtcgc
     1261 cgaacgacct gctctcgctc aagggcgcgc tccaggagat cggtcccctc ggggagacgc
     1321 ttgagcctgc gtcgtcgaga tcttctttgc tctcgcgggt gctcggggcg atggaggagc
     1381 cgccgggggt gcgggagctc atagcggctg ccatctccga ggagagcggt gagatcatcc
     1441 gccccggcta ctcagaggag ctggacgagg cccgcgagtt cagggacggg gcccacgcgt
     1501 ggctgactcg tttcgaggcc gaggagcggc tcaagacggg gctcaagacc ctgaaggtcg
     1561 gctacaggga cggcgagggc tacttcatcg aggtcgccgg caaggagacg agcgcggtcc
     1621 cggagcgtta ccagcaccgc aaggcgctca agcacaactc ccgctacgtc acggtcgagc
     1681 taaaagagca cgaatcaagg atgctcggag cccgcgacga ggtcgagcgg atcgagcgca
     1741 ggatcctctc cgagatccgc gccgccatca aggaggccgc gcccgagttg cagaagatct
//
LOCUS       DATAIR010000438         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_443237, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000438 DATAIR010000000
VERSION     DATAIR010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443237"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..979)
                     /locus_tag="VHM69_10630"
     CDS             complement(17..979)
                     /locus_tag="VHM69_10630"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX2740894.1"
                     /translation="MRTQTTNNTVKEIAEFLKTLDRVAVTTHVGADGDAIGASAALVR
                     LMRRLGATAVFCHEEAVPTYLKWLLPDEALSELPPDHDLLVADTSRADRTGVPVPEAG
                     ARLNVDHHEDNPLYAEYNLVSPRAAATAEIVADLYAELDVPLDRPAAEALYVGIRTDT
                     GGFNFRNISPRAHELVADLLRAGVVPAEVNERINRRGSIEQLRIVGVSLANAVRYGEA
                     LISTVENADYERTGATEIDSKESIDQLRTVEGIDVVAHLREVPQGTKGSLRSETVDVG
                     EIARHFGGGGHRLAAGYTTPRRPQEAKDELLERLEGVLDLGGGR"
     gene            complement(957..1313)
                     /gene="rbfA"
                     /locus_tag="VHM69_10635"
     CDS             complement(957..1313)
                     /gene="rbfA"
                     /locus_tag="VHM69_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX2740895.1"
                     /translation="MSDRTRKVESQLKEITGEEVASLSDPRIQGLVTVTGVRVSPDLA
                     HATVFYSVLAGEDMEAAHAGLQSAAGRIQAAVGAQTRLKRTPRLHFEPDPVVEHATRI
                     EAALREVRIDEDTNDE"
     gene            complement(1310..1591)
                     /locus_tag="VHM69_10640"
     CDS             complement(1310..1591)
                     /locus_tag="VHM69_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX2740896.1"
                     /translation="MFCTVRLELELPFASSLKDKRQTLRSLKDRLRRKNVSVVESDNQ
                     DLWQRATVEIALAAVSRGAAEEKREELRRTLLNYEELSILDWREEFVKL"
     gene            complement(1601..3745)
                     /gene="infB"
                     /locus_tag="VHM69_10645"
     CDS             complement(1601..3745)
                     /gene="infB"
                     /locus_tag="VHM69_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2740897.1"
                     /translation="MSKRVYEIAREQSLESKDVIERLKEAGVEVKSHSSSVDDNVYEQ
                     VFGAGANGETPNGRSEAQEAEAEAERSGHGATIAEAPAEEPVAGPQQSRPQRKRRRVV
                     IDASARNRGARPAAQAPSREPERTPVEEKKEPEKTGVKVEPGATVKDLGEAVGVPPTQ
                     VIKVLMGLGEMKTLTQTLSTEEIELVAEELGVEVEVGAVEEPEPLEVTPDDDPADLVE
                     KPPVVTVMGHVDHGKTSLLDRIRRANVQSGEAGGITQHIGAYQVESDGRKVTFIDTPG
                     HEAFTEMRARGARVTDVVVLVVAADDGIMPQTIEAIEHAKAAGVPMLVALNKIDVPNA
                     NQDRVLGELSERELVPEVYGGDTVTVPVSAKTGEGIDELLENILVVAELEDLKANPKA
                     PASGYVIEGERDPGRGPVATLLLSRGTLQKGDAVLAGTAYGRVRAMLDYTGNRVKDAG
                     PGTPVEILGLSGVPEAGTRFEVVDHERIARDRAQQAEEKLRRQELAQGGTRRTLEELL
                     GEGGTQDLNLVVKADVAGSVEALKEALAKLSTDEVRVNIVRSGVGALTDSDVMLASAS
                     DGIMLGFNVRPTNTAKQVAEREGVEIRTYDVIYKALEEIEAAMRGMLAPEIQERETGT
                     AEVRQTFRVPNVGLVAGCYVTSGEIFRNNRVRVVRDGTIVYEGNIASLKRFKDDARSV
                     REGFECGVGVENFNDVKEGDVLEFFETVEVPR"
     gene            complement(3738..4868)
                     /gene="nusA"
                     /locus_tag="VHM69_10650"
     CDS             complement(3738..4868)
                     /gene="nusA"
                     /locus_tag="VHM69_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX2740898.1"
                     /translation="MNTALLSALHEIETDKGIPFETVKGVLEESLLAAYHGREGAEED
                     ARVVLDEETGDLRVMKDGEDITPHDFTRIAAQVMRQTFYQRLNEIHNEQLLEEYGERM
                     GDVVTGIVQQAHRRMTLVDLGRVEAILPASEQVPNERYENGQRLKVYLLEIREGGRGP
                     SLTVSRRHEGLLRNLFELEVPEIYDGLVEIRAVAREAGLRSKVAVWSKEPGVDPVGAC
                     VGPRGSRVRAVVSELRNEKIDIIQWDPEPARFIAKALSPARVREVYLDEDEKQAEVIV
                     PDDQLSLAIGREGQNARLAVKLTDWKIDIKPESQATEYEEEEEDWEPDEDSTMHRCRA
                     VLSNGRRCANMALPGSLFCGIPSHQAQAAEFEGMVEGRGSDE"
     gene            complement(4865..>5304)
                     /locus_tag="VHM69_10655"
     CDS             complement(4865..>5304)
                     /locus_tag="VHM69_10655"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740899.1"
                     /translation="AIDRLTEVVEGVLPEGTELVEARFSGGPLLTLLLDREDGPVDHA
                     FCSRVASVVAPAVEAEGYDGPIEVSSPGIERPLTKPEHFRRFVGHEAKVKIGEPVDGR
                     RNFTGVIDRTTEDAFILKLSEGAEVELPFGSVTRANLKEDIYK"
BASE COUNT          766 a         1797 c         1655 g         1086 t
ORIGIN      
        1 gatagagccc cgttggttat cgtccgcccc cgagatccag gaccccttca agcctttcaa
       61 gaagctcgtc tttcgcttcc tggggacgtc gaggggtggt gtatcctgcg gcgagcctgt
      121 gccccccgcc tccgaagtgg cgtgcgatct cccctacgtc gacagtctcg gagcggagtg
      181 agcctttcgt accctgtggc acctcgcgca gatgggccac gacgtcgatg ccctcgacgg
      241 tgcgtagctg gtcgatggac tccttcgagt cgatctcggt cgcgccggtg cgctcgtagt
      301 cggcgttctc gacggtggag ataagggcct ccccgtaacg gacggcgttg gcgagtgaga
      361 cgccgacgat cctgagctgc tcgatgctac ccctgcggtt gatgcgctcg ttgacctcgg
      421 cgggtactac tccggcccga agcaaatcgg cgacgagctc gtgggccctc ggggagatgt
      481 tgcggaagtt gaatccgccg gtgtctgtcc tgataccgac gtatagggcc tccgccgccg
      541 gcctgtccag aggcacgtcg agttcggcgt agaggtcggc gacgatctcc gctgtcgcgg
      601 cggctctcgg gctcacgagg ttgtattcgg cgtagagcgg gttgtcctcg tggtggtcga
      661 cgttgagtcg ggcgcccgct tcgggaacgg ggacgccggt cctgtcggcg cgggaggtgt
      721 ccgcgaccag caggtcgtgg tcaggcggga gctcgcttaa tgcctcgtca ggcagtagcc
      781 acttcaggta cgtgggcacc gcctcctcgt ggcagaagac ggctgtggcc ccgagccggc
      841 gcataagacg cacgagcgcg gctgatgcgc cgatggcgtc gccgtcggcc ccgacgtgcg
      901 tcgttacggc gaccctgtcg agggtcttca agaactccgc tatctccttc acggtattat
      961 tcgtcgtttg tgtcctcatc tatccttacc tccctcagag cggcttcgat cctcgtcgca
     1021 tgctcgacca ctgggtcggg ctcgaaatgc agcctgggcg tgcgcttgag gcgcgtctgg
     1081 gcccctacgg cggcctgaat gcggcctgcg gcgctctgca agcctgcgtg agcggcttcc
     1141 atgtcctccc cggccaggac gctgtagaag accgtcgcgt gtgccaggtc tgggctcacg
     1201 cggacgcccg taaccgtcac gaggccctgg atgcgcgggt cggacaacga ggcgacctct
     1261 tcgccggtga tctccttcag ctgcgactcc accttgcgcg tgcgatcact catagcttca
     1321 cgaactcctc gcgccagtcc aggatggaca actcctcgta gttgagaagc gtcctgcgta
     1381 actcctcccg tttctcttcg gcggcgccac gggagacggc ggcgagggcg atctccacgg
     1441 tggctcgctg ccagaggtcc tggttatcag actcgaccac ggagacgttc ttgcggcgca
     1501 ggcggtcctt cagggagcgc aacgtctggc gcttatcctt caggctggag gcgaaaggca
     1561 gctccagttc gagccttacc gtgcagaaca aaagccgggg ttacctcggg acctcgacgg
     1621 tctcgaagaa ctccagcacg tcgccctcct tgacgtcgtt gaagttctcg acgccgacgc
     1681 cgcattcgaa gccttcgcgt acagagcgag cgtcgtcctt gaaccgcttc aacgaggcaa
     1741 tgttgccctc gtagacgata gtgccgtcgc gcacgacgcg aacgcggtta ttgcggaaga
     1801 tctctccact cgtgacgtag caacccgcaa cgaggccgac gttcggcacg cggaatgtct
     1861 ggcgcacctc ggccgtaccc gtctcgcgct cctgtatctc gggtgcgagc atccctcgca
     1921 tagcggcttc gatctcttcg agagccttgt agatcacgtc gtacgtacgg atctccacgc
     1981 cctctcgctc cgcgacctgc ttggccgtgt tggtcggcct gacgttgaag ccgagcatga
     2041 tgccgtcgct ggccgacgcg agcatgacgt ccgagtcggt tagagccccg acaccgctgc
     2101 gcacgatgtt gacgcggacc tcgtccgtag agagcttggc gagtgcctcc ttaagcgcct
     2161 cgacggaacc agcgacgtcg gccttgacga ccaggttcag atcctgggta ccgccctcac
     2221 cgagtagctc ctccagagta cgacgtgttc cgccctgcgc gagctcctga cggcgcagct
     2281 tctcctcggc ctgctgggcg cggtcgcgtg cgatccgctc gtggtcgacg acctcgaagc
     2341 gggtccccgc ctcggggacg ccggagaggc cgaggatctc cactggtgtc cccggtcctg
     2401 catccttgac gcggttgccc gtgtagtcga gcatcgccct cactctgccg tatgccgtgc
     2461 ctgcgagcac cgcgtcgccc ttctgcagcg taccccggct aagtagaagc gtggcgacgg
     2521 gtccgcggcc tgggtcgcgc tcgccttcga tcacataccc gctcgcgggc gctttcgggt
     2581 tggccttcag atcctccagc tcggccacga ccaggatgtt ctcgagaagc tcgtcgattc
     2641 cctctccggt cttcgcagac actggcactg tcaccgtatc gccgccgtag acctctggca
     2701 cgagctcccg ctcggagagc tcgccgagta cacggtcctg gttcgcgttg ggcacgtcga
     2761 ttttgttcag cgcgacgagc atggggacgc cggcggcctt cgcatgctcg atggcctcta
     2821 tggtctgggg catgatgccg tcatcggcgg cgacgacgag gacgacgacg tcggtcaccc
     2881 gcgcgccgcg ggcgcgcatc tcggtgaatg cctcgtggcc aggcgtgtcg atgaaagtaa
     2941 ccttcctgcc gtccgactcg acctggtagg cgcctatgtg ctgcgtgatc ccacccgcct
     3001 cacctgactg cacgttcgcc ctgcgtatgc ggtcgaggag gctcgtcttg ccgtggtcga
     3061 cgtggcccat cacagtcacg acgggcggct tttcgacgag gtcggcgggg tcgtcgtcag
     3121 gtgttacctc gagcggttct ggctcctcga ccgcgccgac ctcgacctcg accccgagct
     3181 cctcggcgac aagctctatc tcctccgtgg agagggtctg agtgagcgtc ttcatctccc
     3241 cgagccccat cagtaccttg atgacctggg tcgggggcac cccgacagcc tccccgaggt
     3301 cctttacggt agcgccgggc tcgaccttca ctcccgtttt ctcaggttct ttcttctcct
     3361 cgacgggcgt ccgttcgggc tccctggagg gggcctgggc ggccggacgc gcgccgcggt
     3421 tgcgcgcact cgcgtctatg acgacccgac ggcgtttcct ctgcggccgg ctctgctgcg
     3481 gcccggcgac aggctcctcc gctggcgcct cagcgatcgt ggcgccgtgc cccgagcgct
     3541 cagcctcggc ctcggcttcc tgtgcttccg agcggccgtt gggagtctcg ccgttggccc
     3601 ctgcgccgaa gacctgctcg tacacgttgt catcgactga ggaggagtgg cttttcacct
     3661 cgaccccagc ctccttcagg cgctctataa catccttgct ctcgagtgat tgctcgcgtg
     3721 ctatctcgta aacgcgttta ctcatcagat cccctaccct ctaccatgcc ctcgaactcc
     3781 gcggcctgcg cctggtgcga cgggatgccg cagaagagtg agcccggaag cgccatgttg
     3841 gcgcacctgc gaccgttgga gaggaccgcc cggcagcggt gcatggtcga gtcctcgtcg
     3901 ggctcccagt cctcttcctc ttcctcatac tcggtcgcct ggctctcggg ctttatgtct
     3961 atcttccagt ctgtcagctt gacggcgagg cgggcgttct ggccctcgcg gccgatggcg
     4021 agtgagagct ggtcgtcggg tacgatgacc tcggcctgct tctcgtcctc gtccaggtag
     4081 acctcgcgta cccgtgcagg gctcagggcc ttcgctataa agcgcgcggg ctcggggtcc
     4141 cactggatga tgtctatctt ctcgttcctg agctcggaga cgacggctcg caccctgctt
     4201 ccgcgcgggc cgacgcaggc tccgacgggg tccacgccag gctccttcga ccacacggcg
     4261 accttcgagc gcagcccggc ttcgcgggcg acggcccgga tctcgacgag gccgtcgtag
     4321 atctcgggca cttcgagctc gaagaggttc ctcagcagcc cctcgtggcg ccggctcacg
     4381 gtcaggctcg gaccgcggcc tccctcccgg atctcgagca ggtacacctt gagccgctgg
     4441 ccgttctcat agcgttcgtt gggcacctgt tcgctggctg gaaggatcgc ctcgaccctc
     4501 ccgaggtcga cgagcgtcat ccgcctgtgg gcctgctgta cgatgcccgt tacgacgtcg
     4561 cccatgcgct caccgtactc ttcgagcaac tgctcgttgt ggatctcgtt gagacgctgg
     4621 tagaaggtct ggcgcatgac ctgggcggct atgcgggtga agtcgtgcgg cgttatgtcc
     4681 tcgccgtcct tcatcacccg caggtcgccg gtctcttcat cgaggacgac cctcgcgtcc
     4741 tcctcggccc cttccctgcc gtggtaggcg gcgagcagcg actcctcgag cacacctttc
     4801 accgtctcga aggggatgcc tttgtccgtc tctatctcgt gcaatgctga caataatgca
     4861 gtattcattt gtatatgtcc tccttgaggt tcgcgcgggt cacggaaccg aacggcagct
     4921 ccacctccgc tccctctgag agttttagta tgaaagcgtc ttcggtcgtc cggtctatga
     4981 cgccggtaaa gttcctgcgc ccatccacgg gctcgccgat cttgaccttc gcctcgtgcc
     5041 cgacgaaacg acggaagtgc tccggcttcg tcagggggcg ttcgatccct ggcgacgaga
     5101 cctcgatcgg accatcgtag ccctccgcct cgactgccgg cgcgacgacg gaggcaacac
     5161 gcgagcagaa cgcgtgatcg accgggccgt cctcccggtc gaggagcaga gtgagcagag
     5221 gtccccctga gaaccgggcc tctaccagct cggttccctc gggaagtacg ccctcaacta
     5281 cctctgtgag cctgtcaatt gccg
//
LOCUS       DATAIR010000439        10940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_397353, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000439 DATAIR010000000
VERSION     DATAIR010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397353"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VHM69_10660"
     CDS             complement(<1..851)
                     /locus_tag="VHM69_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2740900.1"
                     /translation="MAERYDLVVVGAGPGGSSTAYHSTKAGLNTLLVDRQEFPRDKTC
                     GDGLMPHAASEVALMGLADWLDEPHHGKFTGFSIYTRSALLRQNVPPSLHGPRGYVVR
                     RQETDAKVLERAVSAGADFRSGVRATDLVRSASGAVTGILASKAGESLRFDAPLVVVA
                     DGVGGFAGKGMKAHQNAVARRQYFRDVDGPDKKDLHVFITKDMNSHGAGYGWVFYFGD
                     GTANVGAGVSTSALARTGRNLKDFYDRFLEEPLMADWLENATPEGPPTSWSLKMGMWG
                     ARRYGQG"
     gene            950..1156
                     /locus_tag="VHM69_10665"
     CDS             950..1156
                     /locus_tag="VHM69_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740901.1"
                     /translation="MVDRNRPARYEVGDRAALKATRQPVEILEVRRGEFVPDFVYKVR
                     VLAERPARPYNLSVPEHDLSDLSG"
     gene            complement(1306..2358)
                     /locus_tag="VHM69_10670"
     CDS             complement(1306..2358)
                     /locus_tag="VHM69_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740902.1"
                     /translation="MSSTIETLERELGEAQAEREREQRAVVRAENAHEAACSARDEAW
                     SEGSATDPDVEELTGLVYRRRRELYDAIDRLKAAEGKERRIKSRLMYVLPAERRRYLS
                     GAVDEEFEDLLAAVREAYSDVLPDVRVSVVTERPGEVDLRGDATWRALERLYSGTVRR
                     WVEIRSDIDSPEAGRMARQISYYAGGHTPRTFVGPEEFRAGSFRGARELRPLSGYDDP
                     EGRLALARITARRYLDENSVARLGKRPQSLVQVAEIARLYQTMNLTVDEIRWRLEQLI
                     SLRFEVLEAVYRLTGERRRFSFYEIARAARLLDSHFPDSGVAAEVLVQCVDQALSTGV
                     PLVDVAYMNVTTGEEF"
     gene            2552..3217
                     /locus_tag="VHM69_10675"
     CDS             2552..3217
                     /locus_tag="VHM69_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX2740903.1"
                     /translation="MDFNTGSGGTAGSGGSSQGPGGTPPRASGAAGGEFDYTDPVQSF
                     INTVRNVVTAPVNFYRSVPTRGNLLNPLIFAIICYEVSAILGGLLSLAGVYGSEGFGS
                     YIVSLILAPIFAAIGLFIGAGILHLLVMLIIGSRNAGFEGTVRVSAYSSVMSLVSWIP
                     VIGWIASLYGIYLAIVGMREVQNTSTGKAAIVGLIPAVVVHLHILLIIALVGAAIFMS
                     TQQ"
     gene            3328..4332
                     /locus_tag="VHM69_10680"
     CDS             3328..4332
                     /locus_tag="VHM69_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX2740904.1"
                     /translation="MAQTVTLIPGDGIGPDLTDSVKEVIGALDVDIEWEIAEAGETVM
                     DREGTPLPESVLESIRRNEVALKGPLTTPVGTGFRSVNVALRKELDLYGNIRPSLSLP
                     GIETPYSNIDLIIYRENTEDLYAGVEHTVGKHAAESIKIITREGTERICRMAFERSKE
                     QGRRHITVVHKANIMKFTDGLFRDVFFEVAKDYENDFEEIDDRIVDNMCMQLVMKPNL
                     YDVLVCPNLYGDILSDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAGQNRANP
                     LATLLSAKNMLIHIGYAEEADRMQAGIEAALKSPETRTKDLGGSAGTREFTQAIVSNL
                     "
     gene            4455..5576
                     /locus_tag="VHM69_10685"
     CDS             4455..5576
                     /locus_tag="VHM69_10685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2740905.1"
                     /translation="MRIAYFTETFLPATDGVVTRLRHTIDELLELGDEMLVIAPKYGE
                     GPGSYEGVPIHRVSSVPFPPYPQIKLAPINPGVGTALRRFGPDLIHAVNPFVLGWGAS
                     FYSRRMNVPLVASYHTNVAAYASFYKLDLFDRVTRWHTRRLHNQAALNLCTSEATLRY
                     LSREGIERLHLWPQGVDGRRFHPDKFSKDCRVRLTNGHPAERLLLYVGRLSQEKNVGK
                     LKAVLREVPGVRLALVGDGPARRDLEQEYADTPTVFTGALHGGQLAAAFASADVFLFP
                     STTETLGMAMIEALASGLPVLAARTGATGEVVSDGETGLLYDPASDAALVAAVRKLLS
                     DDGLRVQMGRNARAAAETRDWRTSTRTLRGYYEQALGQG"
     gene            5573..6037
                     /locus_tag="VHM69_10690"
     CDS             5573..6037
                     /locus_tag="VHM69_10690"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX2740906.1"
                     /translation="MSEAKARLKRGGKRFSKFTIVGLSNSVIDIGVLNLFLWLQPTRE
                     VYLLVIYNAIALVLANLNSYVWNTLWTFRSRAEHDLRQIVLFALQVLVNVVVSSGLFW
                     ALIHPIIVYTEVPTYLAGNVAKIISVTVASTISFFIMRYVVFSRKRWFKDRL"
     gene            6071..6143
                     /locus_tag="VHM69_10695"
     tRNA            6071..6143
                     /locus_tag="VHM69_10695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6104..6106,aa:Gly,seq:gcc)
     gene            6172..6242
                     /locus_tag="VHM69_10700"
     tRNA            6172..6242
                     /locus_tag="VHM69_10700"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6204..6206,aa:Cys,seq:gca)
     gene            6256..6330
                     /locus_tag="VHM69_10705"
     tRNA            6256..6330
                     /locus_tag="VHM69_10705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6288..6290,aa:Val,seq:gac)
     gene            6907..7071
                     /locus_tag="VHM69_10710"
     CDS             6907..7071
                     /locus_tag="VHM69_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740907.1"
                     /translation="MVETLASLFRRRSEPAFIRSDNGPEFIAKAIKRWLDASGVKTLY
                     IEPGSPWENA"
     gene            7056..7421
                     /locus_tag="VHM69_10715"
     CDS             7056..7421
                     /locus_tag="VHM69_10715"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2740908.1"
                     /translation="MGERLTSETFISRFSDELLKREVFADLLEAKVLVEDYREHCNHH
                     RPHSALGYQTPAEFAVATDLDTKVKDGWKSEELESVLTLRGLGPFACSRMYSVSDQSD
                     QPDGFVSLVLAYYAVESTP"
     gene            7673..8011
                     /locus_tag="VHM69_10720"
     CDS             7673..8011
                     /locus_tag="VHM69_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740909.1"
                     /translation="MLLRKRLTVFVVAAMVLTMMVAAPAFAHQCTNVSKKSGAGSIGT
                     YNVVTETFTPSKSGGGAFITFTDGSTFSYDVYLHDTLPEGALQAGPGGDDECDGKAVD
                     NALVCLGIEA"
     gene            8970..10883
                     /gene="thrS"
                     /locus_tag="VHM69_10725"
     CDS             8970..10883
                     /gene="thrS"
                     /locus_tag="VHM69_10725"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2740910.1"
                     /translation="MASVMLPDGKQLEIEPGEKARDVAEKIGKRLANDAVVAKLDGRL
                     IDLDATLDGGGDFEVITKDSSEGLEVLRHSTAHAMAQAIVELYPGSKLTLGPPIENGF
                     FYDIEVAGRLTDEDLPRIEERMREIVARDLQIKREEVSKAEAEALYEDNPYKTEIVEA
                     LEDGDITVYRQGDFFDLCRGPHVPSTGRLGAFKLQNIAGAYWRGDEKNPMLTRVYGTA
                     WPTEKELKSYLRRLEEAKARDHRKIGKDLELFAFSPDTGAGIPLFLPKGEMIRHLMED
                     YVREVQTRHGYEHVWTGHLVNEVLFAKSGHLEHYRESMFPPMVDGDTRYRLKPMNCPS
                     HMTLYNSRSHSYRDLPLRYAEFATLYRYEKSGELTGLTRVRSLTQDDAHVFCTEDQIQ
                     SEFARALEIVREVLDTYGFAEYRVQLSLRDQDSAKYVADDAKWSRAEKELRDALDTAG
                     IAYDEESGEAAFYGPKADFMAKDVLGREWQLSTIQVDFIQPARLGCEYIGEDGKAHTP
                     VLLHRAVTGSTERFMAVLIEHYAGAFPVWLSPVQAVVIPVADRHIGYASEVRDKLDAA
                     GLRVEVDDSQNSMQKKIRENARQKIPYLLIVGDREAEEGTVNVRRRGEKQQEEMSLED
                     FAGKVSGEVRSRE"
BASE COUNT         2172 a         3309 c         3406 g         2053 t
ORIGIN      
        1 atcccctggc cgtagcgcct ggcgccccac atccccatct tgagcgacca gctcgtcggc
       61 ggaccttccg gcgtggcgtt ctccagccag tctgccatca gcggctcctc gaggaagcgg
      121 tcgtagaagt ctttgaggtt gcggcccgta cgtgcgagcg ccgaggtcga gacccctgcc
      181 ccgacgttcg ccgttccgtc tccgaagtag aagacccagc cgtacccggc gccgtgcgag
      241 ttcatatcct tcgtgataaa gacgtgcagg tccttcttgt caggaccatc cacgtcgcgg
      301 aagtactgcc ttcgcgcgac ggcgttctgg tgggccttca tccctttgcc tgcaaagcct
      361 ccaacgccgt cagcgaccac aacgagggga gcatcgaagc gcagggattc gcctgccttc
      421 gaggctaaga tgcccgtcac cgcacccgaa gcggacctca cgaggtcagt ggccctgacc
      481 cctgagcgga agtccgctcc cgcggagacc gcgcgttcca ggaccttggc gtcggtctcc
      541 tggcgcctga cgacgtagcc cctgggacca tgtagcgacg gggggacgtt ctggcgaagg
      601 agggcggatc gtgtgtatat cgagaatccc gtgaacttac cgtggtgcgg ctcgtcgagc
      661 cagtcggcga ggcccatcaa cgcgacctcg gaggcggcgt gcggcatcag gccgtcacca
      721 cacgtcttgt ccctcgggaa ctcctgccgg tcgacgagca gggtgttcag ccccgccttc
      781 gtcgagtggt aggctgttga ggagccgcca ggaccggctc caacgaccac tagatcatac
      841 cgctcggcca tagtcctcct tctcgaagtc ctcccgactg tgccagaatg tgctccgccg
      901 cgggagcggc gctcattata ctttcggcta cgtacgagga gaaggggact tggtcgaccg
      961 caacaggccc gcgcgatacg aggtggggga ccgcgcagcc ctcaaagcca cccggcagcc
     1021 ggtagagatt ctcgaagtca ggaggggcga gttcgtcccc gacttcgtct acaaggtgcg
     1081 tgtcctcgcc gagaggccgg cgagacccta caacctctcc gtgcccgagc acgatctttc
     1141 agacctgtcg ggctgaggct acaggcatca ggtttcaggc atcagcaagt gtgggccgga
     1201 gataatgccc cgcgtcaact acacgctgtg tcgcggtagc gttttggatg ccgcctcttc
     1261 gacggaccgg gcgtcgaaga acaaaacctg aagcctgatg ccttattaga attcctcgcc
     1321 cgtcgtgacg ttcatatagg cgacatcgac caggggcacg ccggtagaca gcgcttgatc
     1381 tacgcactgg acgagaactt cggcggcgac gcccgagtcc gggaagtgac tatccagtag
     1441 acgggcggcg cgggcgatct cgtagaagga gaagcggcgc cgttcgccgg tcagcctgta
     1501 tactgcttcg agcacctcga agcggagcga gatcaactgt tcgagcctcc atcggatctc
     1561 gtccaccgtc aggttcatgg tctggtaaag gcgggcgatc tctgccacct ggacgaggct
     1621 ctgggggcgc ttgcccagac gggccacgga gttctcgtcg agatagcgcc gcgcggttat
     1681 gcgggccagc gccaaccgtc cctcaggatc gtcatagccg gaaaggggcc gaagctccct
     1741 ggcgccgcgg aacgagcctg ccctgaactc ttctggtccg acgaaggtgc ggggggtgtg
     1801 cccccctgcg tagtaggaga tctggcgcgc catcctcccc gcttcgggtg agtcgatgtc
     1861 gctgcggatc tcgacccacc tgcgcacggt gcccgagtac aatcgctcca gcgcccgcca
     1921 cgtcgcgtcg ccccgcaggt caacctcgcc cggcctctcc gtcaccacgg agacccgcac
     1981 gtccggaagc acgtcgctgt acgcctctct cacggccgcg agcaggtcct cgaactcctc
     2041 gtccaccgcc ccggacaggt accgccgcct ctcggcgggg agaacgtaca tgaggcggct
     2101 cttgatcctg cgttccttgc cctcggccgc cttgagcctg tctatggcgt cgtagagctc
     2161 cctccggcga cggtaaacga gcccggtaag ctcctcgaca tcggggtctg tcgccgagcc
     2221 ttcggaccac gcctcgtccc tggcagagca cgccgcctcg tgggcgttct cagcccggac
     2281 aacggcccgc tgttctctct ctcgctccgc ctgggcctcg cccagctccc gctccagagt
     2341 ctctatcgtc gaggacatcc gaaattcccc gttcccgaag aacgcacact actgcaagta
     2401 ttctacagta ccccgtaccc aacgggacag accggaaggg attcatgggc ttgcctaagc
     2461 cgcacgaggc ttgccttcgc ggttgcgcga tgctcccgca acgtgtacat ttctactagt
     2521 tatccgaagt gcgatgggag aggagattca cgtggatttc aacacgggat ctggcggtac
     2581 tgcggggtcg ggaggttcct ctcaggggcc cggtggaacg ccgccgcggg cgtcgggggc
     2641 ggcgggtgga gaattcgact acacggaccc cgtccagagc ttcataaaca cagtcaggaa
     2701 cgtcgtaacg gcacccgtaa acttctaccg gagcgtcccc acacggggaa acctgctgaa
     2761 ccccttgatc ttcgcgatca tctgttatga agtctcggcg atcctcggtg gcctgcttag
     2821 tctggcgggt gtgtatggca gtgagggttt cggctcctac atcgtctccc tgattctcgc
     2881 cccgatcttc gccgccatcg gcctgttcat cggggcaggc atactgcacc tgctcgtgat
     2941 gctgatcata ggctcgcgca atgctggctt cgagggtacg gtaagggtga gcgcgtactc
     3001 ttccgtgatg tccctcgtaa gctggatacc ggtgatcggc tggatcgcga gcctgtacgg
     3061 catctatctg gccatagtgg gaatgaggga ggttcagaac acgtcaacgg gcaaggccgc
     3121 tatcgtgggc ctgatcccag ccgtcgtggt gcacctgcac atcctgctga tcatcgcctt
     3181 ggttggggcc gccatattca tgagcaccca gcagtagcag gacaagcttc ctgcggaagc
     3241 ttccggcttc gtcgccggaa tcgtggttag ccgcttgctt acctgaccgc tatactgtcc
     3301 ccggtctata gcgaaggggg tttgcgcgtg gcgcagacgg ttactctaat accgggcgac
     3361 gggatagggc cggatctcac cgactccgtg aaggaggtca tcggcgcgct cgacgttgac
     3421 atcgagtggg agatagccga ggccggagag acggtgatgg acagggaagg cacgccccta
     3481 cccgagtccg tgctcgaatc tatcaggcgc aacgaggtgg cgctgaaggg gcccctaacc
     3541 acgcctgtcg ggactgggtt tcgatccgtg aacgtggcgc tgcgcaagga gctcgacctg
     3601 tacgggaaca tcaggccgag cctcagcctg cccggcatcg agacccctta cagcaacatc
     3661 gacctcatta tctaccgcga gaacacggag gacctgtacg ccggcgtcga gcacaccgtc
     3721 ggcaagcatg ctgccgagtc gatcaagatc atcacgcgcg agggcaccga gcgcatctgc
     3781 aggatggcgt tcgagcgctc gaaggagcag ggacgcaggc acatcaccgt cgtacacaag
     3841 gccaacatta tgaagttcac cgacgggctt ttcagggacg ttttcttcga ggtcgcgaaa
     3901 gactacgaga acgacttcga ggagatagac gaccgtatcg tggataacat gtgcatgcaa
     3961 ctcgtcatga agccgaacct ctacgacgta ctggtatgcc cgaacctcta cggcgacatc
     4021 ctatccgacc tctgcgccgg actcacgggt gggctcggcg tcgcccccgg ggccaatata
     4081 ggtgaggaga tcgcggtctt cgagcctgtt cacggctcgg ccccgaagta cgcgggacag
     4141 aaccgcgcaa acccgctcgc gacgctgctc tcggccaaga acatgctcat ccacataggc
     4201 tacgccgaag aggcagacag gatgcaggca ggaatagagg ctgctctgaa gagtcctgag
     4261 acccgtacaa aggacctggg cggcagcgcc ggaaccaggg agtttacgca ggccatagtc
     4321 tcgaacctgt aggttctgag tccggtcggc aagagtggag gtagcggtga gtgacgggat
     4381 ccgtcgagga gctgttacgc gacgctacgg cggcgtagca gcagtctccg gtcgcgacta
     4441 gagaagaagg ccgcttgcgg atcgcgtact tcaccgagac cttccttccg gccaccgatg
     4501 gcgtggtcac caggctccgg cataccatag acgagctgct cgagttgggc gacgagatgc
     4561 tggtgatcgc gcccaagtac ggagaggggc ctggttctta cgagggggtc ccgatccaca
     4621 gggtctccag cgtgcctttc cctccctatc cccagataaa gctcgccccc ataaatccag
     4681 gcgtcggcac cgcgctgcgc cgcttcgggc cggacctgat ccacgccgtc aacccttttg
     4741 tgctgggatg gggggcgtct ttctactccc ggcgcatgaa cgtcccgttg gtggcttcgt
     4801 accacacgaa cgtcgcggcc tacgccagct tctacaaact cgacctcttc gacagggtca
     4861 cccgctggca cacaaggagg ctgcacaacc aggccgcgct caacctgtgc acttcggagg
     4921 caaccctcag gtacctttcg cgagagggaa tcgagcgtct gcacctctgg ccgcagggcg
     4981 tggacgggag acgcttccac cccgacaagt tctctaaaga ttgccgggtc aggctaacta
     5041 acggccaccc tgcggagcgg ctcctgctct acgtcggacg cctcagccag gagaagaacg
     5101 taggaaagct gaaggccgtt ctccgcgagg tccccggggt gcgtctcgcc ctcgtcggag
     5161 acggacctgc tcgcagggac ctcgaacaag agtatgcgga tacgccgacc gtcttcaccg
     5221 gggcgctaca tggcgggcag ctcgccgccg cgttcgcgtc cgccgacgtt ttcttgttcc
     5281 cttcgaccac ggagactttg gggatggcga tgatcgaagc cctcgcatcg gggctgcccg
     5341 tgctcgcggc aaggacgggc gcgacgggcg aggtggtaag cgacggcgag accggacttc
     5401 tctacgatcc tgcctccgac gcggccctcg tagccgccgt caggaagctc ctctcagacg
     5461 acggtctacg cgtgcagatg ggccgcaatg cccgcgcggc tgccgagacg cgcgactgga
     5521 gaacttcgac gcgtacgttg cgcggatact atgaacaggc gctggggcag ggatgagcga
     5581 ggcgaaggcc aggctcaagc gcggcgggaa acgcttctcg aagttcacca tcgtcggcct
     5641 ctccaactcc gtaatagaca tcggggtgtt gaacctgttc ctgtggctgc agccgacgcg
     5701 cgaggtgtat ctgcttgtga tctacaacgc catcgcgctc gtcctcgcga acctgaacag
     5761 ctacgtgtgg aacacgctgt ggacctttcg tagtagggcg gagcatgacc tgaggcagat
     5821 cgtactcttc gcgctacagg tgctcgtcaa tgtcgtggtg agtagcggct tgttctgggc
     5881 cctcatccac cccattatcg tgtacacgga ggttccgacg tatctggcgg gtaacgtcgc
     5941 gaagatcatc tccgtcacgg tggcttctac catcagcttt ttcatcatgc gctacgttgt
     6001 tttctctaga aagcggtggt tcaaggaccg cttgtaagag ccggaggccc gtgctaaatt
     6061 attgccgcgc gcggaagtag ctcagctggt agagcatcac cttgccatgg tgagggtcgc
     6121 gggttcgaat cccgtcttcc gctctggccg cggggcgaca gcggcgccgc tggcggcatg
     6181 gccgagtggt taggcaaggg tctgcaaaac cctgtacccc ggttcgattc cgggtgccgc
     6241 ctctcggagc agaacgggcg attagctcag ggggagagcg cttccttgac gcggaagagg
     6301 tcaatggttc gaatccatta tcgcccacca ccgtaacccc ccttatctaa ggacaaaaca
     6361 gagagagcgt agaagggcgc cggagcttat ccggcgccct tgtgcagcaa cgcaaccgct
     6421 gcacccgtgc gagcggttgt gcccagcgaa tcggtccgag gatgtggcaa agcgtaaaga
     6481 agacaggagc aagaagagct ccacgcacca gacggagcat ctcgtcgagc gcatagtcgc
     6541 catgatgtag gagcaggagc cgcaggtagt ggtaaagcat gaaaagcaac tgccggcaaa
     6601 acacggcggc aagcgcactc gccagatcga cgtgctcctc aaaggacatt tcgcgggcta
     6661 cccgacgatg ttcatcatcg agtgcaaaaa ccatagttgg cctgtgaatg taggtgacgt
     6721 gggccgtttc cgaggtcttc tcgaggacgt tggtcgttcg ccgcagcaag gagtgccggt
     6781 ctcggctggc agtgttggct tgacctgctc cccgaaaact gatctagcta ctatgagagt
     6841 cctactgccc cagacagcag ggagtgcctg agcatagaag tcgagcgctc gatcaccgcc
     6901 gaggacgtgg tagagaccct cgcatccctt ttccgtcgaa ggagcgagcc ggccttcatc
     6961 cgctcggaca acggccccga gttcatcgcc aaagccatca agcgttggct tgatgcctcc
     7021 ggggtcaaaa ccctctacat cgagccggga tctccgtggg agaacgccta acctcggaga
     7081 cgttcatcag tcgcttttca gacgagctgc taaagagaga ggtgttcgcc gatctgttgg
     7141 aagcgaaggt gctggttgag gattaccgtg agcactgcaa ccaccacagg ccacacagcg
     7201 cattgggcta ccagaccccg gccgagttcg cggtggctac tgatctggac acgaaggtga
     7261 aggatggctg gaagtcagag gagctagaat cggtactcac actgcggggt ctcggccctt
     7321 ttgcgtgttc gcggatgtat tcggtgtctg accagtcaga tcagccggat ggatttgtta
     7381 gtcttgtcct agcttactac gcggttgaat cgacaccgta gcagcgtgga gaccgatact
     7441 gaatttctat gcaagtggta catgataccc attggtgggc gtatgcctcg ctggcgctct
     7501 tacagccata tcggctctgg gctgccgcag tggtcaagtc ccggaggctc aatacgaaaa
     7561 ctccgggaat tatctttctc gtaacgcttg tcgtaacggt tagcagacgt gcgcctgcta
     7621 ggttggtgag gcaaagagga gccccgcccg cgacttaagg aggaacccgc taatgttgtt
     7681 gaggaaaagg ctaacggtct ttgtagtcgc cgccatggtg cttacaatga tggtcgcagc
     7741 tccggcattc gcccaccagt gcaccaacgt gagcaagaaa tccggtgctg gctccatagg
     7801 cacttataac gtagttacag agaccttcac gccatcaaag agcggtggcg gtgcctttat
     7861 cacgttcacc gatgggtcca ctttctccta cgacgtctac ctccacgaca ccttgcccga
     7921 aggtgcgctt caggcgggtc ctggaggaga tgatgagtgt gacggcaaag ctgtagacaa
     7981 cgcgttggtc tgtctgggta tagaggcata agttcaggag aaagaaccgt acatactagg
     8041 ggctctcctt gggccggggc ttctttgccc cggcccttgt gtgtgagcgc gctcgatcaa
     8101 ttttttggtc gtagcgtcag ggtcgatgac ctccactacc tcacccctgc gcttgcgctc
     8161 gacgacgcca gtctcgcacc aactgcgatc tttttgccag tcgaagcccg cctccttgag
     8221 agttttgcag atgatgtagg tgctcaccac cgggagcctc ccgtaaggct cttcgtagcg
     8281 tcgtgagcga ccaggtcgcg gtgccatccc tctctcggtg tggtctgcgg agtacctcgg
     8341 ccaggatgcg ctcgcgctgg gcatccgcgt agatggtcgg agggccaccg ctgtgacgag
     8401 gttcgagggc agcaagtccc tcgcgattga agcgtgaaac cagacgagag accgcttccc
     8461 agagcgccgc ccggcggctt tcgccgcatc ggtgtagctt tgtctattag cgacggcaag
     8521 caaggcttcg gcacgagcga ctgatgcgaa caagccagaa tcgttcctgg tcagtaaggt
     8581 ttcgcagagg gtctttctcg cggcgggtca tcggtaagcc tcccggagaa gaagaggttg
     8641 agcattttgc agcatacccc tcctccgccg gaatggctac gtccatgaca aatgacccac
     8701 tagatctgcc tctcatacgt gcagcgaccg gggttaagaa cctgaaaacc tgggaaaagc
     8761 tatgttgccg atttctcatt gtgggccccg attgtgtaaa attgggcaag gtatggaaaa
     8821 gtatcacaca gctttgacgc ggaagaggtc ggtggttcga aaccacgatg gcccactacg
     8881 ctgcgcgggc ctcgccagcg cactgcctcg aactacctag ccccactcgc gttttccctc
     8941 atccaccctt gcacaagaaa ggtgcgcaaa tggcttccgt catgctaccc gacggtaaac
     9001 aattagagat agaaccgggc gagaaagccc gggacgtcgc cgagaagata ggtaaacgcc
     9061 tcgccaacga cgccgtagtg gcgaagctgg atgggcgcct catagacctc gacgccacgc
     9121 tggacggcgg gggagatttc gaggtgatca ccaaagactc ctccgagggc ctggaggtct
     9181 tgcggcactc gacggcgcac gcgatggccc aggcgatagt cgaactctac ccaggcagca
     9241 agctgacgct cggccctccg atagagaacg gcttcttcta cgacatcgag gtcgcgggac
     9301 ggcttaccga cgaggacctg ccgcgcatcg aggagcggat gcgcgagatc gtcgcgcggg
     9361 acctccagat aaagcgcgag gaggtctcga aggccgaggc cgaggcgctc tacgaggaca
     9421 acccgtacaa gacggagatc gtcgaggcgc tggaagacgg ggacatcacg gtctacaggc
     9481 agggagattt cttcgacctc tgccggggtc cgcacgttcc tagcaccggc aggctcggtg
     9541 ccttcaagct ccagaacatt gcaggggcct actggcgcgg cgacgagaag aacccgatgc
     9601 tgacccgcgt atacggcacg gcctggccga cggagaagga actcaaatcg tatctcaggc
     9661 ggctggagga ggcgaaggcg cgggaccacc gcaagatcgg caaggacctc gagctctttg
     9721 cgttctcgcc tgatacgggc gctggcatcc cgctcttcct gcccaagggg gagatgatac
     9781 ggcacctcat ggaagactac gttcgcgagg tccagacgcg ccacggctac gagcacgtct
     9841 ggacggggca tctggtcaac gaagttctgt tcgcgaagtc aggacatctc gagcactaca
     9901 gagagtcgat gttcccgccg atggtggacg gggatacgcg ttatcggctc aagcccatga
     9961 actgcccaag ccacatgacc ctatacaact cccggtcaca ttcgtacagg gaccttccgc
    10021 tccgctacgc ggagttcgcg acgctgtacc ggtacgagaa gagcggggaa ctgacagggc
    10081 ttacgcgggt gcgcagcctc acgcaggacg acgcgcacgt cttctgcacg gaggaccaga
    10141 tccagtcgga gttcgcgcgt gcgctcgaga tagtacgcga ggttctcgat acctacgggt
    10201 tcgctgagta cagggtccag ctctcgctgc gcgaccagga ctcggccaaa tacgttgcgg
    10261 acgacgccaa gtggtcgaga gcagagaagg agcttcgcga cgcccttgat accgcaggaa
    10321 tagcctacga cgaggagtca ggagaagcag ccttctacgg tccgaaggcc gactttatgg
    10381 ccaaggacgt gctcgggcgg gagtggcagc tctcgacgat ccaggtcgac ttcatccagc
    10441 ccgccaggct agggtgcgag tacatcggcg aggatggaaa ggcgcatact cccgtgcttc
    10501 tgcacagggc tgttacgggc tccacagaga ggttcatggc tgtcctgatc gaacactacg
    10561 caggcgcctt ccccgtttgg ctctctccgg tacaggcggt cgtcatcccc gtcgctgaca
    10621 ggcacatcgg ctacgccagc gaggtcagag acaagctcga cgcggccggg ctacgggtcg
    10681 aggtcgacga ttcgcagaac agtatgcaga agaagatcag ggagaacgcg cgccagaaga
    10741 ttccttatct ccttattgtc ggagacaggg aggccgagga gggcacggtc aacgtgcgca
    10801 ggcgcgggga gaagcagcag gaggagatgt cgcttgagga cttcgccggg aaagtctcgg
    10861 gggaggtccg ctcgcgagaa tagtttcgta tagacacagg acgcgggccc gctgtataat
    10921 catgcgcgta gttggggtcc
//
LOCUS       DATAIR010000440         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_443286, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000440 DATAIR010000000
VERSION     DATAIR010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443286"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHM69_10730"
     CDS             <1..273
                     /locus_tag="VHM69_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2740911.1"
                     /translation="VYLAGHRLGWPGTGSHLVFYRLGALRNGDEITLRDRDGRRYNYR
                     VIDTFLVTPDDTWVTGRVRGRDLLTLQTCTPIPTFEKRLIVRAERV"
     gene            complement(494..1519)
                     /locus_tag="VHM69_10735"
     CDS             complement(494..1519)
                     /locus_tag="VHM69_10735"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX2740912.1"
                     /translation="MDVQKLEETAHELVAPGKGILAADESFGTIGKRFEAVGIESSEE
                     SRRAYREMLFTTDGIGEYLSGVILFDETLRQKTSDGTPIPQVLKDQGIIPGIKVDKST
                     VTLPLSRGEKYTQGLDGLGDLLPEYRDLGARFTKWRAVITIGDGIPTKRCIDANARAL
                     ALYAAFCQENDLVPIVEPEVLIDGAHTIERSYEVTEWTLHSTFDHLYEHGVEFSGMLL
                     KPNMVISGKDAPQQASLEEVASATVECLLRTVPAAVPGIVFLSGGQSDLQATAHLNAM
                     NSLYEGLPWELSFSYARALQGQPMEIWGGDVDKVEAAQRTFRHRAKMNSAARSGSYSE
                     DMEEQAA"
     gene            complement(1611..1748)
                     /locus_tag="VHM69_10740"
     CDS             complement(1611..1748)
                     /locus_tag="VHM69_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740913.1"
                     /translation="MCYSYRDRQMDQEIRRTMREREDRRRREQQKKERAADKDRKLVR
                     A"
     gene            1979..4033
                     /locus_tag="VHM69_10745"
     CDS             1979..4033
                     /locus_tag="VHM69_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2740914.1"
                     /translation="MANRLANETSPYLLQHKDNPVDWYPWGEEALKRAREEDRPVLLS
                     VGYSACHWCHVMERESFEDDETARMMNEHFVNIKVDREERPDIDSIYMAAVQALTRHG
                     GWPMTVFLTPDGAPFYGGTYFPPVPSRGMLSFQQLLLSVADAYENRRDEVMQSAQSVR
                     EYLRASTGAVMPEASSAGTELLDGAARALISQLDRRFGGFGGAPKFPQAMNLEVLLRH
                     YHRTGDRSALDGVELTCRQMAQGGIYDQLGGGFARYSVDAYWLVPHFEKMLYDNALLS
                     RLYLETYQATGDAFYRRIAEETLDYVLRDMTSGEGGFYSAEDADSEGEEGKFYVWTSE
                     ELEANLGPEDAALAARYWDVTERGNFEGKNILHVPRPPEAVADEFGLSPDELWDRIVD
                     IRAKLFAVREKRVRPGRDEKILAAWNGLMLRSFALAARITGREDYREAAVKNATFIVE
                     NLKENGRIHRSYKDGRARFNGYLEDYAMVADGLVALYEATFETRWLVEADTLCDAVSE
                     LFWDEEKRAFYDTPSDHEELVTRPRDVYDNAAPSGTSVATEVLLKLALLLDRGDYRQR
                     AEDVLEELAGGMEKVPGAFGRLLCALDFSTSEPREVAVVGDPEAPDTNALLKTVYESY
                     LPNKVVAGRAQDDDEAAGLIPLLAQRPARGGRATAYVCAHYACQAPTTDPEELSRQLG
                     VR"
BASE COUNT          712 a         1345 c         1291 g          793 t
ORIGIN      
        1 gtctacctcg cgggtcacag gctcggatgg cctgggacag gcagccacct tgtgttctat
       61 cgtctgggcg cgttgcgtaa cggggatgag atcaccctgc gcgataggga cggaaggcgc
      121 tacaactaca gggttatcga cactttcctg gtgacccccg acgatacctg ggtaacggga
      181 cgcgtgaggg gacgtgacct gctcacgctc cagacctgca cccccatacc cacgttcgag
      241 aagcgcctga tcgtgagggc tgagcgagtc tagctttcag cacggcgctt cgcgcctttt
      301 cggccctcgc attcagccgt cagctattcc ggccccgctc tgccgctaac gagcaaccat
      361 tgcctgcgga gatgtggttc gaggtgcgtg ggtagcgctt cgggacgact tgctgagagc
      421 tgagagcctc cgaccgcagg gaggcggcgg gctggaagcg aagcccgaag ggcgtagcgt
      481 gctgaccagc taattacgct gcttgttcct ccatgtcctc gctgtagctg ccggagcggg
      541 cggcgctgtt catcttcgcc ctgtggcgga aggttctctg ggccgcctcg accttgtcga
      601 cgtctccgcc ccagatctcc atcggctgtc cctgcagcgc ccgcgcgtag gagaaggaga
      661 gctcccaggg cagaccttcg taaagggagt tcatggcgtt gaggtgggcg gtcgcctgga
      721 ggtctgactg ccctcctgag aggaacacta tgcctggtac cgccgctgga acggttctga
      781 gcagacactc gaccgtcgca ctggcgacct cttcgaggct tgcttgctgt ggtgcatcct
      841 tgcccgagat gaccatgtta ggtttgagga gcatccccga gaactcgacg ccatgctcgt
      901 agaggtggtc gaaggtggag tgcagggtcc actcggtgac ttcgtaggac cgttcgatag
      961 tgtgggcgcc gtcgatgagc acctccggct cgacgatggg gacgaggtcg ttctcctggc
     1021 agaacgcggc gtagagggcg agcgcgcgtg cgttggcgtc tatgcatctc ttcgtcggta
     1081 tgccgtcccc gatggtgatg accgcccgcc acttggtgaa gcgcgccccg aggtccctgt
     1141 actcaggcag caggtcgccc agaccgtcga gtccctgggt gtacttctct cctctggata
     1201 ggggtagtgt tacggtgctc ttgtccacct tgatgccagg gatgatgccc tgatccttca
     1261 agacttgcgg tatcggggtt ccgtccgagg tcttctgacg gagggtctcg tcgaagagga
     1321 tcacacctga gaggtactct cctatgccat ccgtcgtgaa gagcatctcc ctgtaagcgc
     1381 gcctgctctc ctcgctcgac tctattccga ccgcttcgaa gcgcttccct atggtgccga
     1441 agctctcgtc ggcggcgagt atgcctttgc ccggcgccac gagttcgtgg gctgtctctt
     1501 caagcttctg aacgtccatc gcggatctcc ctccgttgga acttacgaca aaggccccgg
     1561 accgatgtcc ggggccctgt gatcatggag ttttgagagg gccccttatg ctaggccctt
     1621 acgagcttgc ggtctttgtc cgccgcgcgt tctttcttct gctgctcgcg gcgcctgcgg
     1681 tcttctctct cccgcatcgt cctgcggatc tcctggtcca tctggcggtc cctgtaggag
     1741 tagcacacgt ccccgctgcc tccttgtcta tgtttcccta tccatagttt tgtctacccg
     1801 cagggtcgcg ggctaaacat gggccgcacc gcgaagcgtc ggccggctac gcgccgcctg
     1861 agtccctatt ttatgccgag catcgtctcc tcggctagag attcaatacc gaaccccccg
     1921 atccgtgata cgttagactt cgcagctaca attcgtttcg ggcgggaggt gagagaccgt
     1981 ggctaacagg ctggcgaacg agacgagccc gtacctgctc cagcacaagg acaaccccgt
     2041 tgactggtac ccgtggggcg aggaggcatt gaagagggcg cgcgaggagg acaggcccgt
     2101 cctgctctcc gtcggctaca gcgcgtgcca ctggtgccac gtaatggagc gcgagtcctt
     2161 cgaggacgat gagaccgccc gcatgatgaa cgagcacttc gtgaacatca aggtcgaccg
     2221 cgaggagagg ccggacatag actccatcta catggccgcc gtccaggccc tgacgcgtca
     2281 cggcggctgg cccatgacgg tgtttctgac tcctgacggc gcccctttct acggcggcac
     2341 gtatttcccg ccggtgcctt cgcgcggcat gctctccttc cagcaactac tcctcagcgt
     2401 cgccgacgcc tacgagaacc gccgcgacga ggtaatgcag agcgcccagt ccgtccgcga
     2461 atacctccgc gcctccacgg gagccgtgat gcccgaggcc agttcggcgg gcacggagct
     2521 gctcgacggc gcagccaggg ccctcatctc ccagctggac cggcgcttcg gcggcttcgg
     2581 aggcgccccc aagttcccgc aggcgatgaa cctcgaagtc ttgcttcgtc actaccaccg
     2641 aaccggcgac cgttcagcgc tcgacggcgt ggagctcacg tgcaggcaga tggcgcaggg
     2701 tggcatctac gaccagctcg gcggcggctt cgcccgctac tccgtggatg cgtactggct
     2761 cgtcccccat ttcgagaaga tgctctacga caacgcactc ctgtccaggc tgtacctgga
     2821 gacgtatcag gccacgggag acgctttcta caggcgcatc gcagaggaga cgctcgacta
     2881 cgtcctgcgc gatatgacga gcggggaagg cggcttctac tcagccgagg acgcggactc
     2941 cgagggcgag gaggggaagt tctacgtgtg gacgtctgag gaactcgagg cgaacctcgg
     3001 tccagaagac gcggccctcg cggcgcgcta ctgggacgtg acggagaggg gcaacttcga
     3061 gggcaagaat atattgcacg tcccgcgtcc gcccgaggcc gtcgccgacg agttcgggct
     3121 ctcacccgac gagttgtggg accgtatcgt ggacatccgg gcgaagctct tcgccgtgcg
     3181 tgagaagcgc gtcaggcccg gacgcgacga gaaaatcctc gcggcctgga acggcctcat
     3241 gctccgatcc ttcgcgctcg ccgctcgtat cacgggccga gaggattacc gcgaagccgc
     3301 cgtgaagaac gcgaccttta tcgttgagaa cctcaaggag aacggccgca tccaccgctc
     3361 ctacaaagac ggccgggcca ggttcaacgg ctacctggag gactacgcga tggtcgcgga
     3421 cgggctcgtc gcgctctacg aggccacctt cgagacacgc tggctcgtcg aggcagacac
     3481 cctctgcgac gccgtatcgg agctgttctg ggacgaggag aagcgcgcct tctacgacac
     3541 cccgtccgac cacgaggagc tcgtcacccg cccgcgcgac gtctacgaca acgccgcgcc
     3601 ctcggggacc tccgtcgcaa cagaggtcct gctcaagctc gccctcctgc tcgacagagg
     3661 cgactaccga cagcgcgctg aggacgtcct cgaagagctc gcgggcggca tggagaaagt
     3721 ccccggcgcc ttcgggcgcc tcctgtgcgc cctcgacttc tcgacctctg agccccgcga
     3781 ggtcgccgta gtaggtgacc ccgaagcccc cgataccaat gcgctcctga agacggtcta
     3841 tgaaagctat ctacccaaca aggtcgtagc gggccgcgcg caggatgacg acgaggcggc
     3901 gggtttgatc cccctcctcg cccagcgtcc cgcccgaggt ggacgggcca ccgcctacgt
     3961 ctgcgcgcac tacgcctgcc aagccccgac caccgacccg gaggagctct caaggcaact
     4021 cggcgttcgg tgaaagttat tcaggaccgg acggtcagag gactcttttc ccgtcctatg
     4081 cccttgagtt ctcgtctacg aatgagactt cgtagaggtg cggctcgaac ctctcggcta
     4141 t
//
LOCUS       DATAIR010000441         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_305223, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000441 DATAIR010000000
VERSION     DATAIR010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305223"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHM69_10750"
     CDS             <1..472
                     /locus_tag="VHM69_10750"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX2740915.1"
                     /translation="GLWRHGDWIKVKENGACVIYGRSDSTINRGGIRMGTSEIYSAVD
                     KVDEVADSLVVDIPRNGGSSFMPLFVVLQEGVELDDDLKGRIKGSIKENTSPRHVPNE
                     IFAVPEIPKTLNGKKLEVPVKKILSGTPPEEAASKESLSNPESLDHFAELAGKM"
     gene            662..2023
                     /locus_tag="VHM69_10755"
     CDS             662..2023
                     /locus_tag="VHM69_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740916.1"
                     /translation="MTGSSAQAAAPTPSTVRKVALAAMAGSAIEWYDFFIYLTAAALV
                     FPALYFPAGNEVAGVLASFSTAAVGFVARPIGGALFGHFGDRLGRKPTLVIALLTMGT
                     ATTLVGLLPTYATIGVAAPIILFVLRFMQGLAVGGQWGGAVLLATEYAPADKRGFYGS
                     FAQVGVPIGLVLGNLSFLVLSAVLGEGTFAAWGWRIPFLFSIVLIGVALYIQLRLEET
                     PAFKQLQERQAESGEGAGGSPVIEVLRDHPKQVLLAGGAFFVVNGAFYVMITGMLDYG
                     TRTLGVSQNVMLSAVLISSFAQIFFLTGWSALSDRVGRRPVYLAGAVLLGLWAFPMFW
                     LVNTKSAVLITLALAFGQLFLSMMYGPQAALFSEMFSARVRYSGASIGYQGASVFAGG
                     LAPIIMVWLLETTGTSLSISFYVLAMAAVTFLSVYLITETYEDELTQDQVKESEEEAA
                     TTR"
     gene            2092..2877
                     /locus_tag="VHM69_10760"
     CDS             2092..2877
                     /locus_tag="VHM69_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2740917.1"
                     /translation="MYETVLYEKDGGVATISLNRPEKLNAFDATMHEELYSALDGAAA
                     DEEVRCVVLRGEGRGFSAGADLAEVVREADGDPDLGEYLRNTYSRLVYRMVEIDMPIV
                     AALHGPVYGAGVGIALACDLRIAAESAKFSVAFIKIGLIPDAGVTFFLPRIVGLGRAM
                     QMSMLGDTVDAEEAYRIGLVNEVVSDEELEGETRKRAGHLAAMPTAALGRIKDSLYAS
                     FETDLETALEHEAQGQTFCGYTQDHREGVAAFFEKRTPEFTGR"
     gene            complement(2882..3310)
                     /locus_tag="VHM69_10765"
     CDS             complement(2882..3310)
                     /locus_tag="VHM69_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740918.1"
                     /translation="MSEVERQQRERKGNPYLYLSLGVGFIIAAVMIVATAGTPAAGLG
                     FVIAAAALLGKGASDLQKQRHAVEPPVSKERELLSAIKDNGGSITPAEAAMETSLTVR
                     EADGMLSELAGAGHLAVRSRDGALIYVLPGRRSPELEGRE"
     gene            3500..4219
                     /locus_tag="VHM69_10770"
     CDS             3500..4219
                     /locus_tag="VHM69_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2740919.1"
                     /translation="MTRQEVVPERLAGATAVVIDVFMATTTLLTILENGARRVYPVAS
                     LEEADEVRETLDAASVLRGGEQDAARIEGYDHGPFPEEYAPEVVREKDVIFVTTNGTR
                     AIADTVSADRVLLGNFRNARAVARHLQASGTDSIYLVCAGSAGRFTVEDFLGAATILS
                     YMDVDGWRLNDGAWAALDFAERHRDDVSGVLKQARAGRWFVENDRMDAFDFVADVGAS
                     TVVPEVVEGELRHAGSQGGTG"
     gene            4216..5274
                     /locus_tag="VHM69_10775"
     CDS             4216..5274
                     /locus_tag="VHM69_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX2740920.1"
                     /translation="MSETINVREGEEFDLQVVESYLRTHVDDVPEGELEVSQFPSGAS
                     NLTYLLKVGDWEGVLRRPPLGPVPPKAHDMGRESGILAKLNSVFPLAPKPYFFCEDES
                     VIGAPFYVMERREGVVLDDTFPEGVEPDEELCRGISRTVVDTLVELHAADVQEAGLGD
                     LGRPDGFLDRQTEGWISRYEKAKTEEIEEVEPLTAWLAGGVPESPPPAIIHNDYKLNN
                     LVLDPEDLMDVRAVLDWEMATVGDPLFDLAVSLSYWIEAGDPDELKAVMPTVTTTPGF
                     MTRKDLIDRYAESSGRDLSEMHWYVVFGYFKLAGILQQIYARWKNGQTTDERFATFGE
                     RVRTLILHAENLSRTGEV"
     gene            5274..>5399
                     /locus_tag="VHM69_10780"
     CDS             5274..>5399
                     /locus_tag="VHM69_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2740921.1"
                     /translation="MDTIGVLGTGTMGAGIVQLAAQSGYRVVACDFAVEALERAQV"
BASE COUNT          988 a         1624 c         1780 g         1007 t
ORIGIN      
        1 aggattgtgg cgccacgggg attggatcaa ggtgaaggag aacggcgcct gcgtcatcta
       61 cggtcgctcc gactcgacca taaaccgcgg cggcataagg atggggacga gtgagatcta
      121 ctccgccgtc gataaggtcg atgaggtcgc cgacagcctc gtcgtggaca tcccgaggaa
      181 cggtggaagc tcgtttatgc cgctgttcgt ggtcctgcaa gaaggcgtcg agctcgacga
      241 cgacctgaag ggcaggatca aagggagcat caaggagaac acctcgccaa ggcacgtgcc
      301 gaacgagatc ttcgccgtcc ccgagatacc gaagaccctg aacggcaaga agctcgaggt
      361 gccggtaaag aagatcctct cgggcacccc gccggaggag gccgcgagca aagagtcgct
      421 gagcaacccg gaatccctcg accacttcgc ggagttggca gggaagatgt agccacgcaa
      481 gccaggagcg gttcgcgcct ggcagtgacc ttgatgaacg attgactctc gtctggtggt
      541 cctataacct gcaccaaggg attgagtggg aaagggggaa tcgtatagcc cggttttaga
      601 gcgctactca cgagtcgtgc ccgcgagggt tgttcggaaa acgggtttga gggaggcatg
      661 aatgacaggt tctagtgcgc aggcggcggc gccgactccg tccacggtgc ggaaggtggc
      721 gctggccgcg atggctgggt cggcgatcga atggtacgac ttctttatct atctgacggc
      781 ggcggcgctc gtctttccgg cgctctactt ccctgctggc aacgaggtag ccggggtgct
      841 ggcgtcgttc agcacggccg ccgtaggttt cgtggcacgt cccataggag gtgcgctctt
      901 cgggcacttc ggggacaggc tcgggcgcaa accaaccctg gtcatcgcgc ttttgaccat
      961 gggcacggcg actacgctcg tcggtctgtt gccgacctac gccaccatcg gggttgcggc
     1021 cccgatcatc ctgttcgtac tgcgctttat gcagggtctc gctgtaggcg gacagtgggg
     1081 tggggcggtg ctccttgcta ccgagtacgc ccccgcggac aagcggggtt tctacggcag
     1141 cttcgcgcag gtgggtgttc ccatagggct cgtactcggc aacctgagct tcctcgtact
     1201 gtcggcggtt ctcggagagg gtaccttcgc cgcctggggc tggcgcatac cgttcctgtt
     1261 tagcatagtg cttatcgggg tggcgcttta catacagttg cgcctggagg agactccggc
     1321 gttcaagcag ttgcaggaga ggcaagcgga gagcggcgag ggcgcgggcg gttcgcccgt
     1381 gatcgaggtg ctcagggacc atcccaagca agtactgctc gctgggggtg cgttcttcgt
     1441 cgtcaacggg gcgttctacg tgatgatcac gggcatgctc gattacggca caagaaccct
     1501 cggagtgtcc cagaacgtga tgctttctgc cgtcctgatc tccagcttcg cccagatctt
     1561 cttcctgacg ggctggtccg cgctttcaga cagggtcggg cgacgacccg tgtatctggc
     1621 cggcgcggtg ctcctgggac tctgggcttt ccccatgttc tggctcgtga acacgaagtc
     1681 ggcggtgctc atcaccctgg cactcgcctt cgggcagctc ttcctgagca tgatgtacgg
     1741 gccgcaggcg gcgctcttct ccgagatgtt cagcgcgcgg gtgcgctact ctggggcctc
     1801 cataggctac cagggcgcct cggtcttcgc gggcggcctc gcgccgatca tcatggtctg
     1861 gctgctcgag accactggca cctcgctctc catctccttc tacgtcctgg ctatggcagc
     1921 cgttaccttc ttatccgtct acctcatcac ggagacttac gaggatgagt tgacccagga
     1981 tcaagtcaag gagagcgagg aggaggctgc taccacgaga tagcttcgcc accattccgt
     2041 ttctctgcgt gcccctccgc tgcggggggc acctttctcg aaggagggaa gttgtacgag
     2101 accgttctct acgaaaagga tggcggggtg gcgacgatct ccctgaaccg gcctgagaaa
     2161 ttgaacgcct tcgacgcaac gatgcacgag gaactgtact ccgccctcga cggcgccgct
     2221 gcggacgaag aagtccgctg tgtagttctc cgtggcgaag gccggggttt ctcagccggc
     2281 gctgacctgg ccgaggtcgt gcgggaagca gatggagacc cagacctcgg tgagtacctg
     2341 cgcaatactt acagtcgtct ggtgtacagg atggtagaga tagacatgcc catagtggcc
     2401 gctctgcacg gcccggtcta cggggccggg gtgggaatag cgctggcgtg cgacctgcgg
     2461 atcgccgccg aaagcgcgaa gttctcggtc gcctttatca agatcgggct catacccgac
     2521 gcgggtgtga cgttcttcct gccgcgcatc gtcggcctcg ggagggcgat gcagatgagc
     2581 atgctcggcg atacggtcga cgcggaggaa gcgtacagga tcggtctggt gaacgaggtc
     2641 gtctccgatg aggagttaga aggggagact cgaaagcgcg ccgggcacct cgccgcgatg
     2701 ccgacggcgg cgctgggcag gatcaaagat tccctctacg ccagcttcga gacggacctg
     2761 gaaacggccc tcgaacacga ggcccagggc cagacgttct gcggctacac gcaggaccac
     2821 agggagggcg tcgcggcgtt cttcgagaag cgaacgcccg agttcacggg ccggtaggct
     2881 cttactccct gccttccagc tccgggctgc gtctgcctgg cagcacgtag atgagggctc
     2941 cgtccctgct ccgtaccgcc aggtgccctg ctcccgcgag ctccgagagc atcccgtccg
     3001 cctcgcgcac ggtaagcgaa gtctccatcg ccgcctcagc gggcgttatg ctgccgccgt
     3061 tgtcctttat ggccgacagt agctccctct ccttgctgac gggtggttcc acggcgtgcc
     3121 gttgcttctg caggtcggat gcgcccttgc cgagaagggc cgcggcggcg atcacgaacc
     3181 cgagaccggc agcgggagtc cctgcagtcg cgacgatcat cacggcggcg ataatgaagc
     3241 cgacgccgag agacaggtag aggtaagggt tgcccttgcg ctctctttgc tgccgctcca
     3301 cctcgctcat tacgcctcca gatcgccggt aacccgaacc ttattctaga tcgtgcgacc
     3361 cagggtgcaa gccggagttc atgaactcat gatgtgctgc cgcgagggcc gacgtggttt
     3421 tgtcctgcgt gttttctgta gaatagtgcg ggtcttctac acgggggttg gagattattt
     3481 gaagaggctc agggttctta tgacgcgtca ggaggttgtg cctgagaggc tcgccggcgc
     3541 aaccgccgtc gtcatcgacg tgttcatggc gactacgacc ctgctgacca tcctggagaa
     3601 cggcgcgagg cgcgtctacc cggttgcgag cctcgaagag gccgatgagg tcagggagac
     3661 gctggatgcg gcgagcgtac tgcgcggcgg cgaacaggac gccgcgcgaa tagagggcta
     3721 cgaccatggc cccttccctg aagagtacgc gcccgaggtc gtcagggaga aagacgtgat
     3781 cttcgtgacc acgaacggca cccgcgctat cgcagatacc gtatcagcgg acagggtcct
     3841 gctcgggaac tttcgcaacg ccagggccgt tgcccgtcac ctccaagcct cgggtacaga
     3901 ttccatctat ctcgtctgcg ccggttcggc gggacggttc accgtcgagg acttcctcgg
     3961 cgcggccacg atactctcgt acatggacgt ggacggctgg cgattgaacg acggcgcctg
     4021 ggcggcgctg gacttcgccg aacgccaccg tgacgacgtc tctggcgtgc tgaagcaggc
     4081 acgggccgga cgttggttcg tcgagaacga caggatggac gccttcgact tcgtcgccga
     4141 cgtgggcgct agcaccgtgg tccccgaggt cgtggaaggg gaacttcgcc acgcagggag
     4201 tcagggagga acgggatgag cgagactata aacgttcgcg agggcgagga gttcgacctc
     4261 caagtggtgg agagctatct gaggacacac gtggacgacg tcccagaagg cgaactcgaa
     4321 gtcagccagt tcccctctgg cgcctcgaac ctcacgtacc tgttgaaggt cggggactgg
     4381 gagggcgtgc tgcggcgtcc gccgctaggt cccgtcccac ccaaggcgca cgatatgggc
     4441 agggagtccg gcatcctggc gaagctcaac tccgtcttcc cgctagcacc gaagccgtac
     4501 ttcttctgcg aggacgaatc ggtcataggc gctcccttct acgtgatgga gcgcagagag
     4561 ggggtcgtgc tcgacgacac cttccctgag ggcgtagaac cggacgaaga actgtgccgt
     4621 gggatctcgc gcaccgtcgt tgacacgctc gtcgagctgc acgccgccga cgtccaggag
     4681 gcagggctcg gggaccttgg caggcccgat gggttcctcg atcggcagac ggaaggttgg
     4741 atctcccgct acgagaaggc gaagaccgaa gagatcgaag aggtcgagcc cctcaccgcc
     4801 tggctcgcag gcggcgtccc cgagagcccg cctcctgcga tcatccacaa cgactacaaa
     4861 ctgaacaacc tcgtcctcga ccccgaagac ctcatggatg taagggccgt cctcgactgg
     4921 gagatggcga ccgtcgggga cccgctcttc gacctcgctg tctcgctctc ttactggatc
     4981 gaagcaggcg accccgacga gctgaaggcg gtcatgccga ccgtgacgac gacaccgggc
     5041 ttcatgacac gcaaagatct catcgaccgc tacgcagaat cgagcgggcg tgacctctcc
     5101 gagatgcact ggtacgtcgt attcgggtac ttcaagctgg cagggatatt gcagcagatc
     5161 tacgcccgct ggaagaacgg ccagaccacg gacgaacgct tcgcgacttt cggagagagg
     5221 gtcaggaccc tgatcctgca cgcagagaac ctctcccgca cgggcgaggt ctagtggaca
     5281 cgataggcgt actcggcacc ggcacgatgg gcgctggcat cgtccagctc gcggcccagt
     5341 caggctacag ggtggtcgcg tgcgacttcg ctgtcgaagc cctcgaaagg gcccaggtc
//
LOCUS       DATAIR010000442         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_27909, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000442 DATAIR010000000
VERSION     DATAIR010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27909"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VHM69_10785"
     CDS             complement(<1..314)
                     /locus_tag="VHM69_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2740922.1"
                     /translation="MGRRARAGDEPARRELIEKNLRLVVSVAKRYRGYGLPFEDLIQE
                     GNVGLMKSVERFDPERGFRFSTYATWWIRQAVGRAVADKGRTIRLPVHAYERLTKVRR
                     VQA"
     gene            complement(565..768)
                     /locus_tag="VHM69_10790"
     CDS             complement(565..768)
                     /locus_tag="VHM69_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2740923.1"
                     /translation="MTVARVTELSSTSTESFEDAIEKGVERATQTLRNVQSAWVKEQE
                     VRVGEDGKISAYQVNMLVTFVLE"
     gene            complement(1044..>1762)
                     /locus_tag="VHM69_10795"
     CDS             complement(1044..>1762)
                     /locus_tag="VHM69_10795"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX2740924.1"
                     /translation="RGFGHQVCTDYVERFLEAAPKYGIELVDRQLAASPIQSPEGESY
                     LKAMASAANFAFANRQLIAHLTREAFRKAGFDAGEHHLSVLYDLAHNNAKFEEHGGRR
                     LLVHRKGATRSFGPGGEDVPEEYRPFGQPVLVPGDMGRYSFVLAGTDGAMRETFGSGP
                     HGAGRRMSRHRAKKVARGRNIVREMGDRGIVLRAAGRATVDEEIPEAYKDAADVIETT
                     HGAGIGRKVARLRPIIVIKG"
BASE COUNT          290 a          630 c          490 g          352 t
ORIGIN      
        1 gcttgaaccc gccgaacctt cgtgagtctc tcgtaagcgt ggacgggaag ccggatcgtg
       61 cgtcccttgt ccgccacagc gcggcctacg gcctgacgga tccaccacgt cgcgtaagtc
      121 gagaacctga agcccctctc cgggtcgaac ctctcgacgg acttcatcag gccgacgttg
      181 ccttcctgga tgagatcctc gaacggtaga ccgtagccgc ggtacctctt ggccactgag
      241 acaaccagcc tgagattctt ctcgatcagt tcccgccggg ccggctcgtc gccggccctc
      301 gctcgtctgc ccaactccgc ctcctcttcg ggccgaagca gcttcctttg tcctatacac
      361 tccaggtaat cggcgagcac gtcccgttcc tccgctcagc gtgaccgtca actatcatcc
      421 gataccggcg tctgataccg gtgtcctcgc gccctcgacg acgattcgag caccccaccg
      481 gcgcgcgcga gacccttgat ttctcacagc gcccgagacc ggctggggat tctatgggct
      541 ctcggacacc cacgggcact tcacctattc caggacaaag gtgacgagca tattgacctg
      601 gtaggcgctg atctttccgt cctcgcccac gcgcacctcc tgctccttga cccaggcgct
      661 ctggacgttc ctcaaggtct gggtcgcccg ctcgacaccc ttctcgatgg cgtcctcgaa
      721 gctctcggtc gaagtggagc taagctccgt gaccctcgct accgtcatcg ccttcctcct
      781 ttcgatcttc gccgcacccc ggactccttc gctccggtga agccttaccg taagggatgc
      841 cgccccgtcg ggcatgatca gcctgactca aatagctggt caggattgac caaagatcct
      901 ttcccaagag gatgattccg gcaacggtca acgagaacgg ccgagattaa ctccacaacc
      961 gcgccgtcgt tcaccggatt acagtcacgg gcacgccggc ttgacttaga gactacggtc
     1021 caccgaccga gaacgtttcc cgctcatccc ttgataacta tgatgggccg aaggcgcgcg
     1081 accttgcggc cgatcccggc cccgtgcgtc gtttcgatga cgtcggcggc gtccttgtag
     1141 gcctcgggga tctcctcgtc caccgtggca cggcccgcgg cccgcagtac gatcccccta
     1201 tctcccatct cccggactat gttgcgccct ctcgccacct tcttcgcccg gtggcggctc
     1261 attctcctgc ccgcgccgtg gggcccggac ccgaaagtct cgcgcatggc gccatccgtg
     1321 cccgccagaa cgaacgagta gcggcccatg tcccccggca ccagcaccgg ttggccgaac
     1381 ggccggtact cctccggcac gtcttccccg ccggggccga aggaccgggt agcccccttg
     1441 cggtggacga gcaatcttct ccccccgtgc tcctcgaact tggcgttgtt gtgcgcgaga
     1501 tcgtatagaa cgctcagatg atgctccccg gcatcgaagc ctgctttgcg gaacgcctcc
     1561 ctggtaaggt gcgcgatgag ctggcggttg gcgaaggcga aattcgccgc agaagccatc
     1621 gctttgaggt aggactcgcc ttccgggctc tgtatgggag acgcggcgag ctggcggtct
     1681 acgagctcga ttccgtactt tggagcggcc tccaggaagc gttccacgta gtcggtgcag
     1741 acctggtgcc cgaaccccct cg
//
LOCUS       DATAIR010000443         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_443374, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000443 DATAIR010000000
VERSION     DATAIR010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443374"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHM69_10800"
     CDS             <1..208
                     /locus_tag="VHM69_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2740925.1"
                     /translation="ILLCTHDMGEAEELCDRVGIMVDGKILALERPEELKRRFATNGR
                     VPTLEETFMAATGYSVEEASAEKE"
     gene            213..1049
                     /locus_tag="VHM69_10805"
     CDS             213..1049
                     /locus_tag="VHM69_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2740926.1"
                     /translation="MRGNVKPLWLSEMVAVWGYVQRNYFLTKRYFMWEIVWLVYVTIN
                     AMAITFIGAGVEAAGGTVDKEFLMTYLLVGALLWNYLSMLFDVLSETVSWERWEGTIE
                     YTFMSPSSRITHLLGMGFYAVVYGILQTALTLGVCYLFFDLDLSRANYAGALLVLAVA
                     SVSLVGFGIVAAVLPLLSPEKGQQVSYIVSSVLLLVSGVYYDVSVLPGWMQTIATVSP
                     VTYALQGARAALQDGASVAELWNSIWVLLVMGFVFVPLGLFVFHLGESFAKRTGKLKR
                     SG"
     gene            complement(1156..1731)
                     /locus_tag="VHM69_10810"
     CDS             complement(1156..1731)
                     /locus_tag="VHM69_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX2740927.1"
                     /translation="MRYTLRMAEQVKPHDSLSVGEYLKLEESATVRHEYVGGELFAMV
                     GATKRNNRIIGNISGCLWGAARGGACRVYSESVKLRVSVDVIYYPDVMVACGPEGADP
                     LVEDDPCLVVEVVSPSTETTDRREKLAAYKRMPGLEAYLIISQDRKWVERHFRAEDGT
                     WRRADLVDEGRFPVPCPETELSLARIYEGLE"
     gene            complement(1878..2075)
                     /locus_tag="VHM69_10815"
     CDS             complement(1878..2075)
                     /locus_tag="VHM69_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEX2740928.1"
                     /translation="MHVNPLLRPIRYAWEYLKEISGENAYDRYLTVHDATHPGKPPMS
                     RGEFYRWRQDDKYNNPGSRCP"
     gene            complement(2082..>3287)
                     /locus_tag="VHM69_10820"
     CDS             complement(2082..>3287)
                     /locus_tag="VHM69_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX2740929.1"
                     /translation="RTTEFAFNGQGPVFAGALFPFVFITIACGALSGFHSLVASGTTP
                     KLIQKESQVRVIGYGAMLTESFVAVMAMIAACILDPGVYFAMNANPALLGDTAQSAAQ
                     AVSGFGFSVNAETLNQTAQRVGEESIIGRTGGAPTLAVGMSQILAGLTGILGASAQAF
                     WYHFAIMFEALFILTTVDAGTRVGRFMIQDLVGNVYRPFARTSWLPGNLIASALIVAG
                     WGYFLYAGVNDPLGGINQLFPLFGIANQLLAAVALTVGTTILIKMGKLRYAWVTALPL
                     AWDAAVTLTASWQKIFSSDPRIGFFAQRAEAAQSIEAGKLNDTLIALKAESIDDAHQI
                     LVNTTVDGVLSILFAVMIIIVIADASRVWFGLIRGSKEPDLSEAPWQESRLDSDGNEI
                     EREPVGAGR"
BASE COUNT          604 a         1011 c         1033 g          639 t
ORIGIN      
        1 aatactcttg tgtacacacg acatgggcga ggcagaagag ctgtgcgacc gcgtcggcat
       61 catggtcgac gggaagatac tggccctcga aaggcccgag gagctcaagc gccgcttcgc
      121 cacgaacgga cgtgtgccga cgctggaaga gacgtttatg gccgcgaccg gctactccgt
      181 cgaggaagcc agcgcggaga aggagtaaag agatgagagg caacgtgaag cccctctggc
      241 tatctgagat ggtggccgtc tggggctacg tacaacgaaa ttactttctg accaagcgct
      301 acttcatgtg ggagatagtg tggctcgtct acgtgacgat caacgccatg gcgatcacct
      361 tcatcggggc tggcgtggag gccgccggcg ggacggtgga caaggagttc ctcatgacgt
      421 acctgctcgt gggcgcgctc ctgtggaact acctctcgat gctcttcgac gttctctcgg
      481 agacggtgag ctgggagaga tgggagggga ccatcgagta cacgttcatg agcccgtcta
      541 gccggatcac gcacctgctc gggatgggct tctacgccgt cgtctacggc atcctgcaga
      601 cggcactcac gctcggggtc tgctacctgt tcttcgacct cgacctttcg cgggcgaact
      661 acgcgggcgc cctgctcgtg ctcgccgtcg ccagcgtctc actcgtcggc ttcggcatcg
      721 tcgccgccgt gctaccgctc ctctccccgg agaagggcca gcaggtgagc tacatcgtct
      781 cgtccgtgtt gctgctagtc tccggcgtct actacgacgt gagcgtgctc ccgggttgga
      841 tgcagaccat cgccaccgtg tcgccggtaa cctacgccct ccagggcgcc cgcgcagcgc
      901 ttcaggacgg cgccagcgtc gcggaacttt ggaacagcat ctgggtgctg ctggtcatgg
      961 gcttcgtctt cgtcccactc gggctcttcg tcttccatct cggcgagagc ttcgccaagc
     1021 ggacgggcaa gctcaagagg agtggatgaa ggaggcttca ggtaacaggt ttcagctttc
     1081 ttgtcgatgc ctgacagcgg cgggttgatc ctgcgcgcaa caaatcctga cggctgaagc
     1141 ctgatgcctg tagccttact ctaacccttc gtagatccgc gccagggaaa gctctgtttc
     1201 gggacagggt accgggaagc ggccttcgtc tacgagatcc gccctccgcc aggtgccgtc
     1261 ctcagcgcgg aagtggcgct cgacccactt ccggtcttga gagatgatca gataagcctc
     1321 aaggcctggc atacgcttgt aggctgcgag cttctcgcga cggtctgtcg tctcggtgct
     1381 cggcgacacc acctctacca ccagacaggg gtcgtcttcg acgagcggat cggccccctc
     1441 gggaccacag gcgaccatta cgtcagggta gtagatgacg tcgaccgata cgcgcagctt
     1501 gacgctctcg gaatacaccc gacaagcgcc gccgcgcgcg gccccccaga gacaacctga
     1561 gatgttgcct atgatcctgt tgttccgctt cgtagcgccg accatcgcaa agagctcgcc
     1621 gccgacgtac tcgtggcgga ccgtggccga ctcttcgagc ttcaggtact cgccgacact
     1681 aagggaatcg tgcggcttga cttgctctgc catgcgtaaa gtgtagcgca acgagagtcc
     1741 atactaaacg atcgtgtcgg ctcgaagtcc gtccaaatgc tcctccgcca gagcttcgtg
     1801 ctcgtcctta gcccgacgta acgctgacca tatctgaatg atgccctcgc ggaccgaccg
     1861 gtccgccaga gaccttatca cggacatcgg ctgcctgggt tgttgtactt gtcgtcctgg
     1921 cgccagcggt agaactcgcc gcggctcatc ggcggtttgc ctggatgtgt cgcgtcgtgg
     1981 accgtaaggt agcggtcata agcgttctcg ccggagatct ccttcaagta ctcccaggcg
     2041 tagcgaatgg gtctgagcag cgggttgacg tgcatactct cctaacggcc ggctcccacg
     2101 ggctcgcgtt ctatctcgtt gccgtccgag tcaaggcgcg actcctgcca tggggcttcg
     2161 ctcaggtcgg gctccttgct gccccggatc aggccgaacc acacgcggga tgcgtctgcg
     2221 atcactatga tgatcatgac ggcgaacagt atggatagca cgccgtcgac cgtcgtgttc
     2281 accaggatct ggtgcgcgtc gtcgatgctc tcggctttga gggctatcaa ggtatcgttg
     2341 agtttacccg cctcgatcga ctgtgctgcc tccgctctct gggcgaagaa gcctatccta
     2401 ggatctgagg agaagatctt ctgccagctc gctgtgagcg ttacggcggc gtcccacgct
     2461 agcggcagcg ccgtgaccca ggcgtagcgc agcttaccca tcttgatgag gatggtcgtc
     2521 cctaccgtca gggcgacggc ggcgagcagc tggttcgcga tcccgaagag cgggaagagc
     2581 tggtttatgc cgccaagcgg atcgttcacg ccggcataga ggaagtaacc ccatcccgcg
     2641 acgatcaggg cgctggcgat caggttgcca ggaagccagg aggtgcgggc gaagggacgg
     2701 tacacgttgc cgacgaggtc ctggatcatg aaccgaccga cccgcgtacc agcatcaacg
     2761 gtcgtgagga tgaacaatgc ttcgaacatg atggcgaagt ggtaccagaa agcctgcgcc
     2821 gatgccccga ggatgccggt caggccagcg aggatctggc tcatccccac ggcgagggtg
     2881 ggagcgccgc cggtgcgtcc gatgatggac tcctcgccca ccctctgcgc cgtctggttg
     2941 agggtctcgg cgtttaccga gaaaccgaag ccgctcacgg cctgggccgc cgactgggcg
     3001 gtgtcgccga ggagtgcagg gttggcgttc atggcgaagt acacgccggg gtccaggatg
     3061 caagctgcga tcatggccat cacggccacg aacgactccg tgagcatcgc cccgtagccg
     3121 atcacgcgca cctgcgactc tttctggatc aacttgggcg tcgtccccga agccacgagc
     3181 gagtggaagc cggatagcgc cccgcacgct atcgtgataa acacgaatgg gaagagggcg
     3241 ccggcaaaga caggcccctg gccgttgaag gcgaattccg tggtcct
//
LOCUS       DATAIR010000444         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_243815, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000444 DATAIR010000000
VERSION     DATAIR010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243815"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VHM69_10825"
     CDS             <1..222
                     /locus_tag="VHM69_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740930.1"
                     /translation="RLVPSAFDGGTQGMLFHLGCLVDSAEDHRRQAEEQGLEIQDFVE
                     GPNTLAVFVRGPEGVSVEYVEHKPTFSLT"
     gene            235..1563
                     /locus_tag="VHM69_10830"
     CDS             235..1563
                     /locus_tag="VHM69_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2740931.1"
                     /translation="MRVTVVGGGLAGLVAARRATELGARVTLLEKGDRLGGSFVYSSG
                     YVWAYKDLETFRSEAPGGDVALQRLIVTRLAPGLSWLEDLGAPVLTRETGNPLTTGVR
                     LAPGPTVAALAESTGPEARLFSGTAFDGLAHDPRGQVDGVRAVSDEARWTERADAVIL
                     ASGGFAANPELVRRYIIQGPGEMRLRAHPWSTGEPFQAALSAGARKSAGLDEFYGRNL
                     PVPPAEFPPERFVEVSQLYGRYAVALNVNGERFADEAADWSETALTRETVRQPGLRAW
                     YIVDGAGLHRRVRERTVREMVTTAREAGGVVLEAESLEELAMRLGERGVSQEGVLSTL
                     DKYNTAVAEGKAAGLSPPRSDPAPPVKVPPYFAVEVAPTITHTIGGLAVDEGCRVLRR
                     PDSRPIPGLYAAGVEVGGVSVGGYSSGLAAAVVFGRLAAESAVGDVEQSG"
     gene            complement(1687..>1871)
                     /locus_tag="VHM69_10835"
     CDS             complement(1687..>1871)
                     /locus_tag="VHM69_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740932.1"
                     /translation="SARALVERLRPVAQDLGCENELDGVSEMAAGGTGADRQRAAFMQ
                     RRSFKYVVEDLIAETA"
BASE COUNT          300 a          577 c          659 g          335 t
ORIGIN      
        1 cgtctcgtac cttccgcgtt cgatgggggg acgcagggga tgctcttcca cctcggatgc
       61 cttgtggact cggcggagga tcaccgcagg caggccgagg agcagggttt ggagatccag
      121 gacttcgtcg aaggaccgaa taccctggcg gtcttcgttc gtggccccga gggcgtgagc
      181 gtggagtacg tggagcacaa gcccaccttc tcgttgactt aagagattag tcagttgcgc
      241 gtcaccgtag taggcggtgg ccttgccgga ttggtcgcag cccggcgcgc caccgagctg
      301 ggcgccaggg tcacgcttct ggagaagggc gaccggctcg gcggttcctt cgtctactcg
      361 agcggctacg tctgggcata taaggacctc gagactttca ggtccgaggc ccccggcggc
      421 gacgtcgcct tgcagaggct catcgtgacc cgattggccc ccggtctctc gtggctggag
      481 gacctcggag cccccgtgct cacgcgcgag acgggcaacc cactgaccac gggcgtacgt
      541 ctggcaccag ggcccaccgt ggcagcactg gcggagtcta ccggcccaga ggcgcgtctg
      601 ttctctggta cggccttcga tggtctggcg cacgatcctc ggggacaggt ggatggggtc
      661 agggcggtct ccgatgaggc acgatggacc gagcgtgcgg acgccgtgat cctggcctca
      721 ggtggtttcg ccgccaaccc tgagcttgtg cgtcgctaca tcatccaggg accgggggag
      781 atgcgcctgc gggcgcatcc ctggagcact ggtgaaccct tccaggcagc gctgtcggcg
      841 ggggcgagga agagcgccgg cctcgacgag ttctacggcc gcaacctgcc ggttccgccc
      901 gccgagtttc ctcctgaacg gttcgtcgag gtctcccagc tctacggccg ttacgcggtc
      961 gccctgaacg tcaacggaga acgcttcgcc gacgaggccg ccgactggtc ggagacggcg
     1021 ctgacgagag aaaccgtccg ccagcccgga ctgcgcgcct ggtacatcgt ggacggggcg
     1081 ggtctccatc gacgggtgcg ggagcgcaca gtgagagaga tggtcacgac cgccagggag
     1141 gccggcgggg tcgtgctgga ggccgagagc cttgaggagc tagctatgag gcttggagag
     1201 cgtggagtgt ctcaggaggg ggtcctgagt actctcgata agtacaacac cgcagtagcc
     1261 gagggaaaag ccgccgggct gtcgccgcca cgcagcgacc ccgcgccgcc tgtgaaagtt
     1321 ccgccgtact tcgcggtgga ggtcgcgcca accataacgc acaccatcgg aggactcgcc
     1381 gtagacgagg gctgccgggt gctccgtcgc cccgactccc gccctattcc tgggctttac
     1441 gccgccgggg tcgaggtggg cggcgtctcg gtgggagggt attcgagtgg tctcgccgct
     1501 gccgtcgtgt tcggcagatt ggccgcggag tcggcggtgg gagatgtgga acagtccggc
     1561 tagtcaacaa ttcggctcgt tggctttggg gtgtacgttc cacaaaaagc cccacgtggg
     1621 cggccatggc ccggtggatc taacacaagc tcctgccggc aaactgaccg gttgcctgat
     1681 tacacttcag gcggtctccg cgataaggtc ttcgacgacg tatttgaatg agcggcgttg
     1741 catgaaggcg gcgcgttgtc tatctgcgcc ggttccgccc gcggccatct ccgaaacacc
     1801 gtcgagctcg ttctcgcagc cgaggtcttg cgcgacggga cgcagcctct ctacgagggc
     1861 tctggcggag t
//
LOCUS       DATAIR010000445         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_443394, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000445 DATAIR010000000
VERSION     DATAIR010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443394"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VHM69_10840"
     CDS             complement(<1..561)
                     /locus_tag="VHM69_10840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740933.1"
                     /translation="MNGRLSFIVLLVSQLAATMGFTFVLPFTPIYVQALGVEDAGNAA
                     AWAGVINSSTAVTMALAAPLWGRLGDRVGLKPMLLRATVAGSIVVGLMGLVGSPWQLL
                     ALKTLQGCLTGTVAAATVLVSATAPPARAGARLGTLQMVIFVAAAAGPFFGGVFADLV
                     GIRASFGVTAALLAASAALISVWVKEE"
     gene            complement(990..2735)
                     /locus_tag="VHM69_10845"
     CDS             complement(990..2735)
                     /locus_tag="VHM69_10845"
                     /inference="COORDINATES: protein motif:HMM:NF045160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740934.1"
                     /translation="MRTEREGGGTSFMATMAEVLTRGAMVELAGERYFERGEEYHRDG
                     LARIFAEHEGIVTARVIGTHEYRVKFQAAGDGLRYSCTCPLGVDGEFCKHCVAAGLAW
                     LEQPRDTVDATGPAGVTMDEVKAYLERQDGDALVGIILDRAAEDELLREGLLLRAASE
                     RPEGPDLDAFRHVIEDALNPGGFLDYGSGWEYMQRVEAVLGAISRLLDRGYADEAIGL
                     IEYALGTVESAMDYDSGGHVSSVARDLEELHLTACERAGPEPETLAHRLFQWEINGPY
                     DTFFGASETYAGVLGERGLAEYRRLAGEEWTRVSRLGPGERDPEGYGRRFRISHVMEA
                     LARRSDDVDELVAVKSHDLSRDHTYLEIAELYRQAGRRDDAFAWAERGVREFPDPDPR
                     LREFLAREYRERGRPEDAMEVMWEEFTTRPHLENYRKLKFFTERLEDWQAWRKKAIDH
                     VREQTASLSGSRWSRTDNSTLVRIFLWERRYDEAWREAVEGGCSPGLWLELAAARERD
                     HPEDAITVYRDRIEALIDLKDKGAYREARDLILRVRDLMRRAGHEPEFAAYLESVRAA
                     HRRKRNLMTLLEGIE"
     gene            2872..3189
                     /locus_tag="VHM69_10850"
     CDS             2872..3189
                     /locus_tag="VHM69_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740935.1"
                     /translation="MDFTGTWDVVSSPDFDDEYLRLGGAPYVTLRQNGDFVKGEYRIG
                     VQYGTINGGVHSDFIDFHFGGNDEMEDAFGEGEATMEGERLIFELWQYQGDEYTYICE
                     RRK"
     gene            3326..3892
                     /locus_tag="VHM69_10855"
     CDS             3326..3892
                     /locus_tag="VHM69_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX2740936.1"
                     /translation="MNPVFTVGHSNHSAERFTDLLRRHGIEMVADVRSHPYSRHVPHF
                     NARYLEKILSNDGIGHLFLGGELGGRPEGEIFYDSDGRVNYALVGRSSSFLAGISRLE
                     QEIRTRTVVLLCSEEDPARCHRRLLVGRALEELGFTLRHIRGDGSIQIEGETDGGQPV
                     LFPETEARPRKSIRSASRRRRRPSSSGH"
     gene            3922..4266
                     /locus_tag="VHM69_10860"
     CDS             3922..4266
                     /locus_tag="VHM69_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX2740937.1"
                     /translation="MRLNNTSRLAGFSRQADLPFFLRELCGAEYLHEPRLAPTREILD
                     AYRQGGVWPDYERAFLELLRERRVEETLDRDLFDVPTVLLCSEARPEHCHRRLVLEYL
                     DREWGGIRAVHL"
     gene            4272..4970
                     /locus_tag="VHM69_10865"
     CDS             4272..4970
                     /locus_tag="VHM69_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740938.1"
                     /translation="MRILVNHLTRMQPGYFCVAGVDVGTARHVRPVLRRGRLTTDLLR
                     TNGGPFDIGSVVDLGPTTRAGHPPELEDHRFDPSRARWLLDEDPDDFWDLLDEMAHDS
                     LEEIFGPELELWDESGTVEVGEGQASLGCLRPEEQPWLYVDHRGTVRLVLDYLMPSVD
                     LSVNDLRLYEKDHRTPRRDLVSSMQSRLAAGVEVILSVGLTRPWRKRGDDAELHWLQV
                     NNIHLKDAPLWGPD"
     gene            complement(5055..5717)
                     /locus_tag="VHM69_10870"
     CDS             complement(5055..5717)
                     /locus_tag="VHM69_10870"
                     /inference="COORDINATES: protein motif:HMM:NF037889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX2740939.1"
                     /translation="MPSEAYTKFDQSGKDILMLWQIHAEVARRGKGRRHRADVLNRAA
                     IVFIAACWESYVEDAAREAFNYLLERTSTPDVFPQKVKTLAAKHLREAKDERRIWELA
                     GSGWRNVLIDHRDLILSRWLRDFNTPKSEQVRTLFAEMLNLRDVTSCWVWSDMTSEQA
                     CAKLNDYISIRGNIAHRTKHDEVVHKNMGKDFLSHVVKLVEKTDKALAAHVQTLIKGE
                     LQ"
BASE COUNT         1176 a         1923 c         1778 g         1143 t
ORIGIN      
        1 ctcttctttg acccagacgg agatcaaagc cgcagacgca gccagaagcg ccgccgtgac
       61 accgaacgag gccctgatgc caacgagatc ggcgaacacg cctccgaaga acggtcccgc
      121 cgccgcagcc acgaagatga ccatctgcaa ggtcccaagc ctcgctcccg ccctcgccgg
      181 cggagccgtc gccgacacga gaaccgtcgc cgcggcgaca gtacccgtca ggcatccctg
      241 caaagtcttt agagcgagta gctgccacgg gctcccaacg agccccatca gcccgacgac
      301 gatagatcca gcgacggtcg ccctgaggag catcggcttg agtccgaccc tgtcaccgag
      361 cctgccccag agcggtgcag ctagcgccat cgtcacggct gtcgagctgt tgatgacacc
      421 ggcccacgcc gccgcgttcc ctgcgtcctc cacgccgagc gcctgcacgt agatcggggt
      481 gaatggcaaa acgaacgtga accccatcgt cgcagcgagc tgcgagacga gcagcacgat
      541 gaacgacagc ctcccgttca cctgcaataa cttcattcgc cgcccccgag gtagcggaca
      601 cctgggacgg cgagatctcc tctgataaat gtgcttctgt accaggccgc cgcattggac
      661 ggccctataa ccctatgtag cgtaattctc cttcccggat actccggggc tcgtgagccg
      721 cggcgaatgc cgcaaaatag tcgcggccct acaggccgcg taaatgaggg aggcttcagg
      781 tgctctcaac ccgacgccca atatcctccc gcacattctc gaaaactctc actccggatg
      841 cgtttctcaa aaccttcgct actgagtgta gcaaaggctc catcataaat ccgaccttgt
      901 aactgcgcga ggcgccgatg aggtgaaaga agtcacgtcc tggcggcatg cgtggcgaac
      961 tgctgattcc ccacatccgg tagaggcgct tactcgatgc cctcgaggag cgtcatgagg
     1021 ttgcgtttcc ggcggtgagc ggcgcggacc gattcgaggt aggcggcgaa ctccggctca
     1081 tggccggcac ggcgcatcag gtcccgaacc ctgaggatga ggtcgcgcgc ctcgcggtag
     1141 gctcccttgt ccttgagatc tatgagcgcc tcgatgcggt cccggtacac ggtaatggcg
     1201 tcttcgggat ggtccctctc gcgcgcggcg gccagctcca gccagaggcc gggcgagcat
     1261 cctccctcca cggcctcgcg ccacgcctca tcgtagcggc gttcccagag gaagatgcgg
     1321 accagcgtcg agttatcggt gcgagaccac cgactccctg agagcgaggc ggtctgctcg
     1381 cggacgtgat cgatggcctt cttgcgccac gcctgccagt cctccagccg ctcggtgaag
     1441 aacttgagct tccggtagtt ctcgagatgg ggtctcgtgg tgaactcctc ccacatgacc
     1501 tccatcgcat cctccgggcg gccgcgctcg cggtactctc gcgcgaggaa ctcccgcagc
     1561 cgggggtccg ggtcggggaa ctccctcacc ccgcgctccg cccacgcgaa cgcgtcgtcg
     1621 cgcctgccgg cctggcggta aagctcggcg atctccaggt acgtgtggtc tcgtgagagg
     1681 tcgtggctct tgaccgccac cagttcgtcc acgtcgtccg aacggcgggc gagggcctcc
     1741 atcacgtggg agatgcggaa ccgcctgccg tagccctccg ggtccctctc gcccggaccg
     1801 agccgggaga cgcgtgtcca ctcctccccg gcgagcctcc gatactccgc gaggccccgc
     1861 tctccgagca cgccagcgta ggtctcagat gccccgaaga aagtgtcgta tggaccgttg
     1921 atctcccact ggaagagacg gtgcgcgagc gtctccggct caggccccgc gcgctcgcag
     1981 gccgtgagat gcagctcctc aaggtcccgc gcgacgctgc tcacgtggcc gcccgagtcg
     2041 tagtccatcg cgctctcgac ggtcccgaga gcgtactcga taaggcctat cgcctcgtcc
     2101 gcgtaacctc tgtccaggag cctggagatg gctccgagga cggcctccac gcgctgcatg
     2161 tactcccatc cggagccgta gtccaggaaa ccgccagggt taagggcgtc ttcgatgacg
     2221 tggcggaagg cgtcgaggtc cggcccctcc ggcctctcgg acgcggcccg cagaagcaga
     2281 ccctcccgca gcagttcgtc ctcagccgcc cggtccagga tgatcccaac cagagcgtcc
     2341 ccgtcctgtc gctcgaggta cgccttcacc tcatccatcg tgacgccggc aggtcctgtg
     2401 gcgtccaccg tatctcttgg ttgctcgagc cacgcgaggc cggcagccac gcagtgtttg
     2461 cagaactctc cgtccacccc aagaggacag gtacacgagt agcgcagccc atcgccggcg
     2521 gcctgaaact tcacgcggta ctcgtgggtc ccgatcaccc gcgccgtgac gatcccttcg
     2581 tgctccgcga agatgcgcgc cagaccgtcc ctatggtact cttcgccccg ctcgaaatac
     2641 cgttcccccg cgagctcgac catcgccccg cgggtcaata cttccgccat agttgccatg
     2701 aagctcgtcc ctccgccctc gcgctccgta cgcataagga tatctcagta tcgctcggct
     2761 cttggttgac cgtcctcatc ccgctcaggc ggtggacgcc tccggccggc tggaccccga
     2821 gcccggcggg tatactggtg gcggatgcga ggcgtcggga ggaaaccttt catggacttc
     2881 acgggcacgt gggatgtcgt atcgagcccc gactttgatg atgagtacct gagactgggc
     2941 ggagcgccct acgtcaccct ccgccagaac ggcgatttcg taaagggcga atacaggatc
     3001 ggagtccagt acgggactat taacggcggc gttcacagcg acttcatcga cttccacttc
     3061 gggggaaacg acgagatgga agacgccttc ggtgaaggcg aggccactat ggaaggggaa
     3121 cgcctgatct tcgagctctg gcaataccaa ggcgacgagt acacctacat ctgcgagcgg
     3181 cgaaagtaga aggtctctac accatctggt agagaaaagc gcgaaaaggg tctggggaaa
     3241 acgcagcgta ggcattatcc tcgataccgt ccgcagggtt tcggaagacc gaaaggaatt
     3301 cacgatcgca ggtcaaggga cggaagtgaa cccggttttc acggtcgggc attccaacca
     3361 ttcggcggaa aggttcaccg acctgttgcg gaggcatggg atagagatgg tcgcggacgt
     3421 ccgttcgcac ccgtactcca ggcacgtgcc ccacttcaac gcccggtatc tggagaagat
     3481 cctgtcgaat gacggcatcg ggcacctctt cctcggcggg gagctcggtg gaaggcccga
     3541 aggagagatt ttctacgaca gcgacggccg ggtaaactac gcgctggtcg ggcgttccag
     3601 ttcgttcctc gctggtataa gtaggctgga gcaagagatc aggacccgca cggtcgtcct
     3661 gctctgtagc gaggaagatc ccgcgcgttg ccaccgcagg ctgctcgtcg gtcgggctct
     3721 ggaggagcta gggttcacgc tgcgccacat ccggggcgac ggtagcattc agatagaggg
     3781 tgagacggac ggcggccagc cggttctgtt ccctgagacg gaggcgagac cacggaagtc
     3841 tatacgatcg gcttcacgaa gaagacggcg tccgagttct tcgggacact gagggacgcc
     3901 ggcatcgggc gcctcctcga cgtgcggctg aacaacacgt ccaggctcgc ggggttctcc
     3961 cgccaggccg acctcccatt cttccttagg gaactgtgtg gcgccgagta cctacacgaa
     4021 ccacggctcg ccccaacccg tgagatcctc gatgcgtaca ggcaaggagg ggtatggcct
     4081 gactacgaga gggcattcct cgagctcctg cgggagcgcc gggtcgagga gacgctggac
     4141 agggacctct ttgatgttcc cacggtattg ctgtgcagcg aggccagacc cgagcattgc
     4201 caccggcgtc tggtccttga gtacctggac cgggaatggg gcggcatcag ggccgtccac
     4261 ctgtagagat tttgcggata ctagtaaatc acctgacgcg gatgcagcca ggctacttct
     4321 gcgtggccgg cgtggacgtt ggtacggcgc gccacgtccg ccctgtactc aggcgcggca
     4381 ggctgactac cgatctcctc cgtaccaacg gcggcccctt cgacatcggg tccgtcgttg
     4441 atctcgggcc gacgacacgc gcaggccatc cccctgagct ggaagaccat cgcttcgatc
     4501 catcgcgggc gaggtggctc ctcgatgagg acccggatga tttctgggac cttctcgacg
     4561 agatggcgca tgatagcctg gaggagatct tcggccccga actcgagctg tgggacgaga
     4621 gcggcacggt cgaagtcggg gagggtcaag cctcgctggg ctgcctgaga ccggaagagc
     4681 aaccgtggct gtacgtggac caccgcggca cggtcaggct ggtcctcgac tacctgatgc
     4741 cctccgtgga cctctcggta aacgacctga ggctatacga gaaggaccac aggacacccc
     4801 gccgggatct cgtctccagc atgcaaagcc gcctcgcagc gggcgtcgag gtgattctaa
     4861 gcgtcggcct gacgcgcccc tggcgcaagc gcggcgacga cgccgagctg cactggctgc
     4921 aggtcaacaa catccacctc aaggacgcac cactgtgggg accagattga cgaagagaag
     4981 attcgagtgc ccccaactag gtgagcataa agtgcttcct ctttacgcag ggtaggacat
     5041 gatgactttc tacgctactg aagctcccct ttgatcagcg tttgcacgtg cgcagccaac
     5101 gccttatctg ttttctctac caacttcact acgtgagaca gaaaatcttt acccatgttc
     5161 ttatgaacga cttcatcgtg ttttgtgcga tgagcaatat tacctcgaat cgatatgtag
     5221 tcgttcaact tggcgcatgc ctgttcagag gtcatatctg accagaccca gcatgaagtg
     5281 acatcacgca aatttagcat ttcagcaaat agcgtcctca cctgctcaga ctttggtgtg
     5341 ttgaaatctc ggagccatct actaaggata agatcacggt gatcgattag aacattcctc
     5401 caaccggagc ccgcaagttc ccatattcgc cgttcatctt tcgcttctcg taggtgtttg
     5461 gctgccaacg tcttgacctt ctgaggaaac acatctggag tgcttgtacg ctcaagcaaa
     5521 taattgaaag cttctctagc agcatcttcg acgtaagatt cccaacatgc agcgatgaag
     5581 acgatagctg ctctattcaa cacatccgca cggtgtcgcc tgccctttcc acgcctagcc
     5641 acctctgcgt ggatttgcca tagcatgaga atatcttttc cactctgatc gaacttcgta
     5701 taggcttcgg aaggcatgtc cagtatctag gttgagctat gctatgtctt ctattctgca
     5761 agtactgtgc atacgcgttg cagtagttgt tgcactatcg gaactcatcg caaagcacac
     5821 cttgacaatc gacaacatcg tgcaatcatg cctcaaacat ctaactcacc tcgcacatga
     5881 agaggggcgg gatgtgcatg taggtcgtgc ttggattcgg gccgacggtg tcctgcgcca
     5941 tctggagagc ctcgactacg gtgtcggccc gtccgagccc catgcgcttc gtggtcgcgg
     6001 ggtcgccgcc tacgagtatg
//
LOCUS       DATAIR010000446         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_27959, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000446 DATAIR010000000
VERSION     DATAIR010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27959"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VHM69_10875"
     CDS             complement(<1..683)
                     /locus_tag="VHM69_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2740940.1"
                     /translation="MARPVTLFTGQWADLPLEELARKCGEWGFDGLELACWGDHFDVD
                     KALSEDGYVEGRWEILRRNGLNCWAISNHLVGQAVCDPIDARHQATLPPDVWGDGDPE
                     GVRQRAAEKMKDTARAAARFGVTQVNGFTGSSIWHMLYSFPPNDFAEIERGYEDFAGR
                     WNPIIDVFDEEGVRWGLEVHPTEIAYDFVTTRKALDAIGNREGFGINFDPSHFAHQFL
                     DSSAFIEEFA"
     gene            complement(732..1730)
                     /locus_tag="VHM69_10880"
     CDS             complement(732..1730)
                     /locus_tag="VHM69_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2740941.1"
                     /translation="MWGRSWTGLVAEAPGVELAAVVDPAPEARRSTDAAPAYEVLEEA
                     LAGVECDAVLVASPPGTHHAVAKAALEAGKHVLCEKPLATSLQDAFDLVETAERAERI
                     LMVSQNYRYNAPFRAVQRLIVGGDLGELVSVRVVCRRDLRTQFPPDDFRYSMRHPYVL
                     DMSIHHFDLIRAATGRDISSVFARSWRVPDSPFVHDPAIAALIDLDGGVTVVYEGDWA
                     TREPETSWNGDWEIVGEAGRLLWSGGTGDRGVGEVVLERWGEEPGPVEQPDLEFVERE
                     ATLQALREAVDGGGPPETSAADNVKSLAAMIGCVSSIESGEPVDVAGLVAARGANL"
     gene            complement(1748..>2209)
                     /locus_tag="VHM69_10885"
     CDS             complement(1748..>2209)
                     /locus_tag="VHM69_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2740942.1"
                     /translation="LAVQAVEPWPDCVSISGDEAALRWYEETRMPLFTWSSLAGGFFS
                     GRFTRDNLDSFESYLDRLCVDTYCHEENFGRLDRAHALAEEKGLTVPQVALAYVLDQP
                     LEVFALVGCNTGDEFRANVEAGGIELTPEELTWLENGDTSAPLEEEQTSSA"
BASE COUNT          368 a          757 c          678 g          406 t
ORIGIN      
        1 gcgaactcct cgataaacgc tgaagagtcg aggaactggt gggcgaagtg gctcgggtcg
       61 aagttgatgc cgaagccttc gcggttgccg atcgcgtcga gcgccttgcg ggtcgtcacg
      121 aagtcgtagg cgatctccgt cgggtgtacc tccaggcccc acctgacgcc ctcctcgtcg
      181 aagacgtcga tgatcgggtt ccaccgcccg gcgaagtcct cgtagcctcg ctctatctcg
      241 gcgaagtcgt tgggcgggaa ggaatagagc atgtgccaga tgctcgatcc ggtgaacccg
      301 ttgacctgtg tgaccccgaa acgggccgca gccctggccg tgtccttcat cttctcagcg
      361 gcgcgctggc gcacgccctc gggatctccg tctccccaca cgtcgggagg gagcgtcgcc
      421 tggtggcggg cgtctatggg gtcgcagacg gcctggccga cgagatggtt gcttatggcc
      481 cagcagttca ggccgttgcg tcttagtatc tcccaccttc cctcgacgta gccatcctca
      541 gatagggctt tgtctacgtc gaagtgatcc ccccaacagg ccagctcgag cccgtcgaag
      601 ccccactccc cgcactttct ggcgagttct tctagcggca ggtcagccca ctgtccggtg
      661 aagagcgtta caggtcttgc cacgttcgaa cctcctgttc acttaattca ctcgcgggac
      721 atcgccaacc tctacaggtt ggcgccccgt gctgccacga gcccggcgac gtcaacgggt
      781 tcgccgctct cgatggagct gacgcatccg atcatcgcag ccaggctctt cacgttatcc
      841 gcggctgagg tctcgggagg cccgccgcca tcgacagctt cgcgcagcgc ctgcaacgtg
      901 gcctcccgct cgacgaactc gaggtcaggc tgctccacag gaccgggctc ctcgccccac
      961 ctttcaagca cgacctcgcc cacgccccga tctcccgttc cgccgctcca tagcagccgc
     1021 ccggcctcac cgacgatctc ccagtcgccg ttccacgacg tctcgggctc gcgcgtcgcc
     1081 cagtctccct cgtagacgac cgtcacgccg ccatccagat cgatcagcgc ggcgatggcg
     1141 ggatcatgta cgaacggact gtccgggacg cgccagctac gggcgaacac gctgctaatg
     1201 tcgcggcccg tcgcggccct aatgaggtcg aagtgatgaa tagacatgtc gagtacataa
     1261 ggatggcgca tcgaatacct gaagtcgtca ggtgggaatt gtgtcctgag gtcgcgccgg
     1321 cagacaaccc ggacagatac gagttccccc agatcccccc ccactatcag ccgctgcacg
     1381 gcacggaacg gcgcgttgta gcggtagttc tggctgacca tcaggatccg ctccgcccgc
     1441 tctgccgtct cgacgaggtc gaacgcgtct tggaggctgg tagccagggg tttctcgcaa
     1501 agtacgtgct tgcccgcctc gagcgcggcc ttcgccacag cgtggtgcgt gccaggcggc
     1561 gaagcgacga gcacggcgtc gcactcgacg ccagctagtg cctcctccag gacctcgtag
     1621 gcgggggcgg cgtcggtgga ccggcgcgct tccggggcag ggtcaacgac cgcggccagc
     1681 tcgacgccgg gtgcttcggc gaccagcccg gtccagctac ggccccacat gccgagtccg
     1741 gcctgaacta cgcggacgat gtctgctcct cctccagtgg cgcgcttgtg tcgccgtttt
     1801 cgagccaggt gagctcctct ggggtcagct cgatcccacc tgcctcgacg ttggccctga
     1861 actcgtcgcc cgtgttgcac ccgacgaggg cgaagacttc gagcggttgg tccagaacgt
     1921 aggcgagcgc tacctggggt acggtcagac ccttctcctc tgcgagggcg tgtgcgcggt
     1981 cgaggcgtcc gaagttctcc tcgtgacagt aagtatcgac gcaaagccgg tcgagatagc
     2041 tctcgaaaga gtccaggttg tctcgcgtga agcggcccga gaagaaacca ccagccaggc
     2101 tcgaccaggt gaacagcggc atccgcgtct cctcgtacca cctgagggca gcctcatctc
     2161 cgctgatgct cacgcagtcg ggccacggtt ctactgcctg cacggcgag
//
LOCUS       DATAIR010000447         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_397659, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000447 DATAIR010000000
VERSION     DATAIR010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397659"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHM69_10890"
     CDS             complement(<1..346)
                     /locus_tag="VHM69_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2740943.1"
                     /translation="MDSTNDSLLKNVRFLRLWIGQGTSFVGDAVSMVALVVLVFQITD
                     SASAVGGVLVARLLPTIASPLAGVIADRVDRRMVLVASDLARTVLILGMVFTRDLATI
                     YVLAFLMGLARTL"
     gene            complement(489..1250)
                     /locus_tag="VHM69_10895"
     CDS             complement(489..1250)
                     /locus_tag="VHM69_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2740944.1"
                     /translation="MLTRLDHLVILVRDLDLAAADYERLGFVVTPGGEHADSLTRNAL
                     IPFDDGSYFELVSFLDPEDARDNVWGWRKFVPHEGLIDYCAASDDLSSEVKRLESLGF
                     DVNGPDEGGRRLPDGAEIRWRSARIRQEGRLLPFLIEDLTPRTLRVPGGPAASHPNGA
                     SGICRLEISAPDARTAAASFGLLIESPDDAGTSVRLGDCVLSPVEREDEKKSTGTGGV
                     GPLVVDLVVETQVSAQELDRHLAHGVGIQLRPPES"
     gene            1331..1786
                     /locus_tag="VHM69_10900"
     CDS             1331..1786
                     /locus_tag="VHM69_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740945.1"
                     /translation="MTRSHPGAEDREQALAYLEELQEETMRLAWGEDASAEDRRRIAV
                     AAIIFGRQFEERVAERSADLGEDGLQRFLLDLMSGVVAEFARIEGLDENEAAGFLSEV
                     GTRDHVLEFDEVLDAYEGGEPGRTLDELLQDAVNSRREKAARSRRHGPF"
     gene            complement(1788..1937)
                     /locus_tag="VHM69_10905"
     CDS             complement(1788..1937)
                     /locus_tag="VHM69_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740946.1"
                     /translation="MTAEVWQFLIVILGFSVVVLLWLFVVYLPGRTLERWEDQRPGRG
                     DKGAG"
     gene            2123..2860
                     /locus_tag="VHM69_10910"
     CDS             2123..2860
                     /locus_tag="VHM69_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2740947.1"
                     /translation="MLYQGRDGAVLGEEQIKLLTLVDIFEPLSREEIEAIQWKHLNTS
                     VEEGETFYTPMDLCETLFVLQKGRVRIYRATPEGREFTVAVVGSGTVFGEMVLTGQRL
                     RNSYAQAIEESEVSTMCRADVERLILDKPRVGLQLVHLLGERLAVQETRMEDMALKEI
                     PARLAGLILLLIESEGIRTSTSYKVPSRYTHQQLGSMIGANREAVTRALARLRELGAL
                     TVRHRYIHVEDIEALKRATDDDFFRET"
     gene            2914..3441
                     /locus_tag="VHM69_10915"
     CDS             2914..3441
                     /locus_tag="VHM69_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740948.1"
                     /translation="METSLLRQFQREVERQCQFAMIALQDIDEASANSDGKLFWYSVQ
                     GLLVAVESISRLLWPPNQGASREALRENLGVDDDSPLKPREFVERFLRYDESLEQWHE
                     SSKHRRFFDSYTEPLDVLAETPPEDRFRGYQLERNALLFHGESYEIGPISQALEELQR
                     KAEEEMQKPRFDHDI"
BASE COUNT          806 a         1146 c         1212 g          650 t
ORIGIN      
        1 acaaggtgcg tgccaggccc ataaggaagg ccagtacgta gatggtcgcg aggtccctgg
       61 tgaagaccat ccccagaatg agaaccgtcc ttgcgaggtc gctcgccacg aggaccatcc
      121 tgcggtcgac gcggtccgcg atgacgccgg ccaacgggct cgcgatggtg ggcaggagcc
      181 tggcgacgag tactccgccc accgccgagg cgctgtccgt gatctggaac acgaggacaa
      241 cgagcgcaac catggagacg gcatctccta cgaaagatgt cccctggccg atccagagtc
      301 gcaagaagcg aacgttcttt agaagagagt cgtttgtgga gtccatgccc ccaactcctg
      361 gagcgagacc gtgcctgact ccggtcgaat atcctcccgg cgaccggagc cactactcag
      421 cttaaccgat gatgcttgta cgcgtctcta gttcatgatt atttggtcgg acgttatccc
      481 ctgttgcttt agctttcagg cggccgaagc tggatgccga ccccgtgggc gaggtgccgg
      541 tcgagctctt gcgcgctcac ctgcgtctcg accaccagat ccacaaccag aggaccaact
      601 cctcctgtgc ctgtgctttt cttctcatcc tcgcgctcta ccggcgagag cacgcaatcg
      661 ccaagccgta ccgaggtgcc ggcatcgtcc gggctctcta tcagcaagcc gaatgaggcc
      721 gccgcagtcc gcgcatccgg cgcggagatc tcgagccggc agatgcctga cgcgccgttc
      781 gggtggcttg ctgccgggcc gcccgggacg cgcagcgtgc ggggggtcag gtcttctatc
      841 aggaacggca ggagcctgcc ctcctggcgt atcctggcgc tgcgccagcg tatctctgcg
      901 ccgtctggaa ggcgacggcc accttcgtca gggccgttca cgtcaaagcc cagggattcc
      961 agcctcttta cttcggacga gaggtcgtcg gaggcggcgc agtaatctat gaggccctcg
     1021 tgcgggacga actttcgcca gccccagacg ttgtccctgg cgtcttcggg gtccaggaaa
     1081 gagaccagct cgaaataaga tccatcatca aaaggtatca gggcgttgcg ggtcaggctg
     1141 tcggcgtgct cgccgcccgg ggtcaccacg aagccaagac gctcgtagtc ggccgcggcg
     1201 aggtcaaggt cgcggacgag gatcacaagg tggtccagcc tggtcaacat gacacgctcc
     1261 cctcgcgtga cgggtccatt agtaagggtg ataccatttt cgcaccggct acgaaaagga
     1321 tgagcaagca atgactcgtt cacatcccgg cgcggaagac cgggagcagg ctctcgccta
     1381 tctggaagag cttcaggaag agacgatgcg cctggcgtgg ggagaggacg cctccgccga
     1441 ggaccgccgc cggatagccg tcgcggccat aatatttggc cgccagttcg aggaacgtgt
     1501 agcggagcgg tccgctgatc tcggcgaaga cgggctgcaa cgcttccttc tggacctgat
     1561 gagcggtgtc gtcgcggaat tcgcccggat cgaggggctg gacgagaacg aggcggcggg
     1621 cttcctgagc gaggtgggca ccagggacca cgtgctcgag ttcgatgagg tgctggacgc
     1681 ctacgaggga ggagaaccag ggcgtactct ggacgagcta ctacaagacg ccgtgaacag
     1741 ccgcagggag aaggcggcaa ggtccaggcg ccacggaccc ttctgaacta cccggccccc
     1801 ttgtcgccgc gtcccggacg ctggtcctcc cagcgctcca gggtgcgccc cggaagatag
     1861 acgacgaaga gccacaacag gacgacgacg gagaacccca ggataactat caggaattgc
     1921 cagacctccg ccgtcaaagt cctcctctcc gtcgcaggtg cgaagccggg cagtagtatc
     1981 gttcatcagt gatgcgtccg ccgtaaaaag attaccccgg ttgaatggaa tggcgatgac
     2041 tggcccgtga ggtagatcgt ggtgtacgta cgtctaaggt tgggtagatc tagcccggat
     2101 aatatccgag cgagacggag ccatgctata tcaagggcga gacggcgcgg tactagggga
     2161 agagcagatc aaactcctca cgctcgttga catcttcgaa ccgctctcgc gggaggagat
     2221 agaggcgatc cagtggaagc atctcaacac gagcgtcgag gagggcgaga ccttctacac
     2281 accgatggac ctgtgcgaga cgctcttcgt gctccagaag ggtcgagtgc ggatctacag
     2341 ggccaccccc gagggacggg agtttacggt cgccgtggtg ggaagtggga ccgtcttcgg
     2401 ggagatggtg ctcacaggcc agcgtctcag gaactcctac gcccaggcta tagaggagtc
     2461 ggaggtctcg acgatgtgcc gcgccgacgt ggagcgtctc atcctcgaca agcccagggt
     2521 cggcctgcaa ctcgtacacc tccttggcga acgtctggcc gtccaggaga cccgcatgga
     2581 ggacatggcc ctcaaggaga tccccgcccg cctggctggc ctgatcctct tgctcatcga
     2641 aagcgagggc atcaggactt ccacgagcta caaggtccct agccgctaca cacaccagca
     2701 actcggcagc atgatcgggg ccaacaggga ggccgtgaca cgagccctcg ccaggctcag
     2761 ggagctcggc gcgctgacag tgaggcaccg ttatattcac gtcgaagaca tcgaggccct
     2821 gaagagggcg accgacgatg atttcttcag ggagacctga gtaggagccc ggtattgtag
     2881 accggcgaac gttcgaggag ggtgggcagg ttagtggaaa catcattgct ccggcagttc
     2941 cagagagagg tcgagcgcca gtgccagttc gcaatgatcg cgctgcaaga catagatgag
     3001 gcctcggcca acagcgacgg caagctgttc tggtactccg tgcagggtct gctcgtcgcc
     3061 gtcgaaagca tctcgcgcct gctctggcct ccgaaccagg gcgcatcaag agaggcgctc
     3121 agggagaacc tcggcgtcga cgatgactcg ccgctcaaac cgcgggagtt cgtggaacgc
     3181 ttcctccgct acgacgagag cctggagcag tggcacgagt cctcgaagca ccgcaggttc
     3241 ttcgactcct acaccgagcc actggacgtg ctggccgaga ccccacccga agaccgcttc
     3301 cggggctacc aactcgaaag gaacgccctg ctcttccacg gcgagtccta cgagatcggt
     3361 ccgatctccc aggccctgga ggaattgcag cgcaaggccg aggaggagat gcagaagccc
     3421 cgcttcgacc acgacatctg aagacggtcg cgcagcagag aaacgctccg aacgcagccg
     3481 agggccccac aaccttccga agcggtatcc ctagctcggg acgcgagcac tactctcgct
     3541 tccggctttt cgctgtgttt tgaaacacgg aatccagtat gtgatgaacc gttttgtgct
     3601 ccagggcaca gaagaatttc tgaggggttt gaacgacgtg cgtcgtggca acaagcatta
     3661 gcaagccgct cgtaaaagcg cggctcatga cgtgcaaatg cctacacatg gaggcaagga
     3721 tggatcacga agcgcgcagt gacaggttca cggaagtaga ggaccggtac gcagggtaca
     3781 cggtctacga ccaggactac gagaagatcg gcaa
//
LOCUS       DATAIR010000448         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_443618, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000448 DATAIR010000000
VERSION     DATAIR010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443618"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VHM69_10920"
     CDS             complement(<1..137)
                     /locus_tag="VHM69_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2740949.1"
                     /translation="MFGGLFGRDVAIDLGTANTLVFVKGQGIVLSEPSVVAIDTKTDK
                     VV"
     gene            complement(176..841)
                     /gene="radC"
                     /locus_tag="VHM69_10925"
     CDS             complement(176..841)
                     /gene="radC"
                     /locus_tag="VHM69_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX2740950.1"
                     /translation="MKQLPPELRPRERLLAEGPEALSSAELLGILFGIGSKEKTAVEL
                     AGQVISESGDLFGLHNVSVHELLGMHGIGEAKACITLAAVEFGKRLGRVRNPGRPVIS
                     SPEDIDGLLRGRIANLDRENFVAVLLNTKNEVIEFPTISVGTLSASLVHPREVFKPAI
                     RASAAGIVLAHNHPSGKVGPSREDREVTRRLKEAADIIGIEILDHVILGDGYFSMKEH
                     GIL"
     gene            complement(934..1506)
                     /locus_tag="VHM69_10930"
     CDS             complement(934..1506)
                     /locus_tag="VHM69_10930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX2740951.1"
                     /translation="MQFVLASESARRVDLLHSAGYDFEARKSGFPEVTLEDPVKTVLA
                     NARGKALAVAGDPDKVVLAADTIVYLSDLPADDCVLGQAKNGADVASFINLLQGRAHE
                     VHSGVAVARGDEISVRHAVTEVRMRRLEAAEVESYVRLGEGVGKAGGYAIQGRAAAFV
                     EWIGGDYTNVVGLPLALAGRMLAGFGVPRP"
     gene            complement(1512..1913)
                     /gene="ndk"
                     /locus_tag="VHM69_10935"
     CDS             complement(1512..1913)
                     /gene="ndk"
                     /locus_tag="VHM69_10935"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2740952.1"
                     /translation="MEETLVLVKGDGVRRRLVGEIIRRIEAKGMDLGDLKLMDVSREL
                     AESHYAEHREKPFFEELVEFITSTPVVAMRVRGEGAIKVMRTLMGVTNPAEAAPGTIR
                     GDLALSMPDNLVHGSDSPESANRELGLFFGN"
     gene            complement(1924..2418)
                     /locus_tag="VHM69_10940"
     CDS             complement(1924..2418)
                     /locus_tag="VHM69_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2740953.1"
                     /translation="MKALLVLQQDPDPFQIISNFLESPILRISGQLVALFFVVLWVAL
                     VYWTYTDASRRGAMSILWGIVAVFFPYVGTLVYLIVRPHEYLEESRERELELAVLERE
                     LRNSVLLCPNCRNLVERDYLICPTCTWELKKSCINCDKPLNMEWGMCPYCGTDQRSGK
                     TVIR"
     gene            complement(2506..>2852)
                     /locus_tag="VHM69_10945"
     CDS             complement(2506..>2852)
                     /locus_tag="VHM69_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740954.1"
                     /translation="AAVAAVRGAYGGLPLGVVFGVLREKDAASMLTGLGGDSHVIVLT
                     RPESERASDPARLQREHGPQDHEGRRALVVHDPVEALGVAVEAVREVAGVVLVTGSLS
                     TAAPVLRWLRET"
BASE COUNT          540 a          947 c          804 g          561 t
ORIGIN      
        1 acgactttat ccgtcttggt gtcgattgct actaccgagg gctcggagag tacgatcccc
       61 tgccccttga cgaacacgag cgtgttcgcc gttcccaggt ctatcgcaac gtcccgcccg
      121 aaaagtcctc caaacatacg cgccccatcc tccgtatcga acaactctgc cgcagctaga
      181 gtattccgtg ctccttcata ctaaagtacc catcgcccag tatcacgtga tccaggatct
      241 cgatgccgat tatgtcggcg gcctccttga gcctcctcgt gacctcccgg tcctcgcggc
      301 tcggtccgac cttgccgctt gggtggttgt gggcgagcac tatgcccgcc gcactagccc
      361 ggatcgccgg tttgaatacc tccctgggat gaaccagaga cgccgatagc gtgccaaccg
      421 agatcgtagg aaattctatg acctcgttct tggtattcaa gagtacggcg acgaaattct
      481 cccggtcgag attagcgatc cggccccgca aaagaccgtc gatgtcctcg ggcgatgata
      541 tcacggggcg tccggggttg cgaacccttc cgagcctttt cccgaactct acggcggcga
      601 gcgttatgca cgccttggcc tctccgatgc cgtgcatccc gagcagctcg tgtactgaaa
      661 cgttgtgcag accgaagagg tccccgctct cggagatgac ctgaccggcg agctcgaccg
      721 cggttttctc tttactccct ataccgaaga gaatgccgag caactcagcg ctcgacagcg
      781 cctcaggccc ctcggcgagc agccgttcgc gcggcctgag ctccgggggg agctgcttca
      841 tggtgtacct gcgccgctcc gtcactatgt cttatatcgt gcacgtcgac gaattacttt
      901 acgtcacgcg atccgcaccg gaaccccgac gcctcaaggg cgtggaaccc cgaatccggc
      961 gagcatccgg ccggcgaggg cgagcggcag cccgactacg ttggtgtaat cgcccccgat
     1021 ccactccaca aacgccgcgg cccgcccctg gatggcgtag cctccagcct ttcccacacc
     1081 ctctccaaga cgaacgtaag attcgacttc ggctgcttcg agccgcctca tccggacctc
     1141 agtaacggcg tggcgcaccg agatctcgtc tccacgcgcg acggctaccc cggaatggac
     1201 ctcgtgagcg cgcccctgca acagattgat gaaactcgca acgtctgcgc catttttggc
     1261 ctgtccgagg acgcagtcat cggcgggaag gtctgacagg tagacgatcg tgtcggcggc
     1321 gaggacgact ttgtcggggt ctccggcgac ggcgagggcc ttccccctcg cgtttgccag
     1381 aactgtcttt acggggtctt cgagcgtcac ctcggggaag ccgctcttac gcgcctcgaa
     1441 gtcgtagccg gcggaatgga ggaggtccac ccgcctggct gactctgagg cgaggacgaa
     1501 ttgcacccgt tctagtttcc gaagaagagc ccgagctccc tgtttgcgct ctccggcgag
     1561 tcggagccgt ggacgaggtt atccggcatg ctcagggcga ggtccccccg gatcgtgcca
     1621 ggggccgctt cagccgggtt cgtcaccccc atgagcgttc tcatcacctt tatggcaccc
     1681 tcgcccctca ccctcatggc gacgaccggg gtcgaggtga tgaactcgac cagctcctcg
     1741 aaaaaaggct tctcgcggtg ttctgcgtag tgtgattcgg ccagttcccg gctcacgtcc
     1801 atcagcttca ggtccccgag atccatgccc ttcgcttcga tgcgacggat gatctcacca
     1861 acgagcctgc gtcgcacacc atcgcctttg acaagtacca gagtctcttc caaagttctc
     1921 tccttatcta atgaccgttt tgccgctgcg ctggtctgtt ccgcagtagg gacacatccc
     1981 ccactccatg ttgagcggct tgtcgcagtt tatgcaggac ttcttcagtt cccacgtgca
     2041 ggtggggcag atcaggtaat ccctctcgac caggttgcgg cagttcgggc acagaaggac
     2101 gctgttcctg agctcgcgtt cgagcacggc cagctcgagc tcgcgttcgc gagactcctc
     2161 caggtactcg tgtggacgta cgatcaggta caccagagta ccgacgtagg gaaagaagac
     2221 ggccacaatg ccccagagta tgctcatggc accgcggcga gaggcgtccg tgtacgtcca
     2281 gtagacgagc gcgacccaca agacgacgaa gaaaagtgcg acgagctggc ccgagatcct
     2341 cagtatcgga ctctccagaa agttggaaat gatctggaac gggtcggggt cctgctgcag
     2401 cacaagcaat gctttcatag gataaacctt atcatcatgt aatagcctag aataaatgac
     2461 acgacggcga ccaccaggag gaggccggtc aactgccagg cgcccttatg tttcacgcaa
     2521 ccaccgcaat accggcgcgg cggtggagag agagcccgtg accagtacca ctccggccac
     2581 ttctctcacc gcctcgacgg cgacgccgag ggcttccaca gggtcatgta ctaccagggc
     2641 tctccttcct tcgtggtcct gcgggccgtg ctcgcgctgt aagcgcgcgg ggtccgatgc
     2701 cctctcgctc tcagggcggg tcaacactat cacgtgggag tcccccccca accctgtaag
     2761 catacttgcg gcatccttct ctcgcaagac gccgaagacg accccgagcg gcagcccgcc
     2821 gtaggcgccg cgcacagccg ccaccgccgc cg
//
LOCUS       DATAIR010000449         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_305526, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000449 DATAIR010000000
VERSION     DATAIR010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305526"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VHM69_10950"
     CDS             complement(<1..245)
                     /locus_tag="VHM69_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2740955.1"
                     /translation="MPELPEVTVISEDVAALARGREVLRAGVFRPDVTNVDPEEFGRR
                     LVGRTLKSTGRRGKIIVLDFGEVVGLVHLVISGRVLRL"
     gene            complement(246..1142)
                     /locus_tag="VHM69_10955"
     CDS             complement(246..1142)
                     /locus_tag="VHM69_10955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX2740956.1"
                     /translation="MEIPGSNYFGSTTYWKVALEHDNTKHELDFDLGAFDLDGTVLRR
                     NLRITHKTVAALERLREKGMRLVVATGRRFEGAREHAARLGFADNDPLICYGGSMVRR
                     MNGETLLHRTLPNNLSVEVLEWAAARDLHARVFVDGHIITSPDTSAAQEHLRRNAEPD
                     VSVVDSPADWLRNGGVEPTKLVVVDHPDDIGGWLGKARAAFEGRLFVTRSLPHYVEVG
                     GLEGTKSKALGFLCERWGIEPGRVLAFGDADNDIDMLRFAGHGVAVGGMTGEVRDAAD
                     EVVPDVDEDGVALYVEKLLGGS"
     gene            complement(1235..>4206)
                     /locus_tag="VHM69_10960"
     CDS             complement(1235..>4206)
                     /locus_tag="VHM69_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2740957.1"
                     /translation="PVPGLDLTPFGFLLSGAMLSWGLFRYRLLDIVPVAHQAIVEGMR
                     DGVVVVDLRGRIVEINPSALSILDVSAPEAIGRAAAEIIPAFGALLEDDGRSVEAGHV
                     ELVRAGQREYELSFALLKDHSGRNTGHLLVLHDITERKRAEEEIRRLNEDLEKRIEER
                     TRDLEDAIADLQESEERYALVVEGSNDGIYDWNIRTGELYWNDRLFEMFGLSRSEFTP
                     TFEGFLGYVHPDDRQRLLDNVSAHLEHDAEFDMELRYRHSSGEYRVCDSSGKAQRDED
                     GVPTRMAGIATDITERKRAEEEIRHLNETLEQRVEERTSQLADAVSGLEMARNEADAA
                     SRAKSEFLANMSHEIRTPMNGVIGMTSLLLDTDLSEEQSEYAETVRVSGENLLTIIND
                     ILDFSKIEAGKMELETVDFDLQRVVEETVDMFAERTQAKGLELASLIERGVPTGLRGD
                     AGRVRQVIVNLLGNAVKFTEKGEVILRVSLDDARDGAVVVRFEVSDTGIGLTEEQRER
                     LFQSFTQAESSTTRRYGGTGLGLAISKQLVELMGGEIGVESEQGKGSTFYFTALLKRQ
                     PASARIPSPSRADLRDLRVLVVDDNETNRKIVHEQVVSWGMENGMAGDGPEALNKLRA
                     AAENGEPYDLAILDMDMPEMDGMELATRIRAQSTIASTRLILLTSIGLRGEADQARRA
                     GFAAYLTKPVGQSRLYDAIATAIGALPAGQEAGGRRGRDAVVTSLSGERHSRGHVLVA
                     EDNPVNQKVAVKLLEKLGFRADVVANGLEAVEAVEHIPYSAVLMDVQMPEMDGYAATR
                     EIRRRQSGKDRTPIIAMTANAMQDDREKAIEAGMDDYVPKPVKPEQLEATLQRCISTA
                     EDEAIDPEASIGRDAEGNSLDRSILDGLRGLQQEGEPDIVNELISLFLTEVPSKLVAL
                     REAVAAGDAGTVERVSRALEENSANMGAVKLEALCTELEAMARSGDLAAAQALIPRLE
                     EELGRVRVAFRKDVSKN"
BASE COUNT          728 a         1443 c         1160 g          875 t
ORIGIN      
        1 aggcgcaaca cccttccgga gatcacaaga tgcacgagcc cgacgacctc cccgaagtcg
       61 aggacgatga tcttgccccg ccgtccggtg ctcttcagcg tccgtccgac gagtcgccgc
      121 ccgaactcct cgggatctac gttggtcacg tcgggcctga agacgccggc ccgcaggacc
      181 tcacgcccgc gtgctagcgc ggccacgtcc tctgagataa ccgttacttc gggcaactcc
      241 ggcacctacg atcctcccag cagtttctcc acgtagaggg cgacgccgtc ctcgtccacg
      301 tcgggcacta cttcgtccgc ggcatcgcgg acctcgcccg tcataccgcc gacggcgacc
      361 ccgtgtccgg cgaagcggag catgtctatg tcgttgtcag cgtcgccgaa ggcgaggacg
      421 cgcccgggct ctatccccca ccgctcgcac agaaacccaa gggccttcga cttcgtgccc
      481 tcaagcccgc cgacctcgac gtagtgcggg aggctgcgcg taacgaagag ccgtccttcg
      541 aaagccgccc gagccttccc gagccaaccc cctatatcgt ccgggtgatc cactaccacg
      601 agcttcgtcg gttctactcc accgttcctg agccagtccg cgggagagtc tacaaccgaa
      661 acgtccggct cggcgttgcg ccgcaggtgc tcctgcgcag cggaggtatc cggcgaggtg
      721 ataatgtgcc cgtcgacgaa gacgcgggcg tgaagatccc gcgcagccgc ccactcgagc
      781 acctccacgc tcaggttatt gggcagcgtg cggtgcagca gagtctcccc gttcatccgc
      841 cgcaccatcg accctccgta gcagatcagg gggtcgttat ccgcgaagcc gagcctcgcc
      901 gcgtgctccc tggccccctc gaagcgcctc cccgtcgcca cgacgagccg catccctttc
      961 tccctcagcc tttccagggc ggccacggtc ttgtgtgtga tccgcaggtt ccgcctcaac
     1021 acggtcccat cgaggtcgaa ggcgccgaga tcgaagtcca gttcgtgctt cgtattgtcg
     1081 tgttccagag caaccttcca gtatgtcgtt gagccgaagt agttggaacc cgggatctcc
     1141 aactcaagac ggtattatcg ccgatgttcg ccggcgttca aaagcactat ttgacgatga
     1201 ccatgtagac caaaccgtgc gtccagaaag tcacctagtt cttcgagacg tctttccgga
     1261 aagctacgcg aacccgtccg agctcctctt ccagacgagg gatcagggcc tgcgcggctg
     1321 cgaggtcccc ggagcgcgcc atcgcctcaa gctccgtgca aagggcctcc aacttcacgg
     1381 cacccatgtt ggctgagttc tcttcgaggg cacgcgaaac ccgctcgacg gtgcctgcat
     1441 caccggccgc cacggcctcc cgcagggcga ctagcttgga cggaacctcg gtcaagaaca
     1501 gcgagatcag ctcgttcacg atgtccggct ctccctcttg ctgcagcccg cgcaggccgt
     1561 ccagaatact cctgtctaat gagttccctt ccgcgtctcg cccgattgag gcttcgggat
     1621 cgatcgcctc atcctcagcc gtggagatac agcgttgcag ggtcgcctca agctgctcgg
     1681 gctttacagg cttcggcaca taatcgtcca tccctgcttc gatggccttc tccctgtcgt
     1741 cctgcatcgc gttggccgtc atcgcgatga tcggtgtgcg atccttgccc gattgccttc
     1801 gtcttatctc cctggtagcc gcatacccgt ccatctcggg catctgcaca tccatgagaa
     1861 ccgccgaata cgggatgtgc tcgaccgcct caacggcctc caggccgttc gccactacgt
     1921 ccgccctgaa tccaagcttc tcaagcaact ttaccgccac cttctggttc acggggttgt
     1981 cctcggccac cagaacgtgc ccgcgcgagt gccgctcgcc ggagaggctg gtgactacgg
     2041 cgtcccgacc cctcctcccc cctgcctcct gcccggcagg cagcgcgcct atcgccgtgg
     2101 caatggcgtc atagagcctc gactgaccaa ccggcttggt caggtatgcc gcgaagcccg
     2161 ctctgcgggc ctgatctgct tcccctcgca agcctatcga ggtcaggagt atgagcctgg
     2221 tcgaagcgat agtggattgc gcccttatcc tggtcgccag ttccatgccg tccatctccg
     2281 gcatgtccat gtcgaggata gccaggtcgt agggctcacc gttctccgcg gcggcgcgca
     2341 gtttgtttag tgcttcgggc ccatccccgg ccatcccgtt ttccatgccc caggagacga
     2401 cctgctcgtg cactatcttg cggttggtct cattgtcatc aacgacaagc actcgaaggt
     2461 cacgcagatc ggcgcggctt ggggagggga tccgggcgct cgccggttgc ctcttcagca
     2521 gtgcggtgaa gtaaaaagtg ctccccttgc cttgttcgct ctctacgcct atctctcctc
     2581 ccatcagttc gaccagttgc ttgctgatgg cgagcccgag ccccgtgccc ccgtagcggc
     2641 gggtggttga agactcggcc tgcgtgaacg attggaacag gcgctcccgc tgctcttccg
     2701 tgagcccgat gcctgtatcc gagacctcga aacgcacgac cacggccccg tccctggcat
     2761 cgtcgagcga gacgcgcagg atcacctctc ccttctcagt gaacttgacg gcgttgccga
     2821 gcaggttgac gatgacctgc cgcacgcgtc ctgcgtcgcc cctgaggccg gtaggcactc
     2881 ctcgttcgat caggctggca agctccaggc ccttggcctg tgtccgctcg gcaaacatat
     2941 cgaccgtttc ttcaactacc ctctgcaggt cgaaatctac cgtttcgagc tccatcttgc
     3001 cggcctctat cttggagaag tcgagtatgt cgttgatgat ggtcaggagg ttctcgcccg
     3061 agacgcgtac cgtttctgcg tactcacttt gctcctctga taggtcggtg tcgagcagga
     3121 ggctcgtcat gcctatgacg ccgttcatgg gggttcttat ctcgtggctc atgttcgcca
     3181 aaaactcgct cttggctctg ctcgccgcat cggcctcgtt cctcgccatc tccaacccac
     3241 tgacggcgtc tgcaagctgc gaggtgcgct cttctacccg ctgctccagg gtctcgttga
     3301 gatgccttat ctcttcttcc gccctcttac gctctgtgat gtcggtagcg atgccggcca
     3361 tgcgtgtcgg cactccatct tcatcgcgct gggcttttcc gctgctgtca cagacgcgat
     3421 actcgccgct ggaatgcctg tagcgtaact ccatgtcgaa ctcggcatca tgctccagat
     3481 gggcgctaac attatccagt agcctctgac gatcatcagg gtgaacgtat cccagaaaac
     3541 cctcgaaagt cggggtgaac tcagagcgcg agaggccgaa catctcgaag agcctatcgt
     3601 tccagtagag ctctccagta cggatgttcc aatcgtagat accgtcgttc gagccttcca
     3661 ccacgagggc gtagcgctcc tcgctctctt gaaggtcggc tatggcatct tcgagatccc
     3721 tggtgcgttc ttcgatccgc ttctccaggt cctcgttgag gcgccttatc tcctcctctg
     3781 cccgcttgcg ctcggtaata tcgtgcaaaa cgagcagatg gcccgtgtta cgtcctgagt
     3841 ggtccttaag caacgcgaag gagagttcat actcgcgctg ccccgccctt acaagctcta
     3901 catgcccggc ttctaccgat cggccatcgt cctccagaag agcaccgaaa gcaggtatga
     3961 tctccgctgc ggcccgcccg atcgcttcag gagcagagac atccaggata ctcagggcgg
     4021 agggattgat ctctacgatg cgccccctga ggtccacgac cacgacccca tcgcgcatgc
     4081 cctcgacgat agcctggtgc gccacaggta cgatatccag cagacggtag cggaaaagcc
     4141 cccaggagag catcgcacca ctgagcaaga agccgaaggg cgtcaggtcg aggccgggta
     4201 ccgggg
//
LOCUS       DATAIR010000450         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_305534, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000450 DATAIR010000000
VERSION     DATAIR010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305534"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VHM69_10965"
     CDS             complement(<1..189)
                     /locus_tag="VHM69_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740958.1"
                     /translation="MKPRVTRVQITQYGRWLPTARIAWVVAAVLVVGTYGAAMPVAYA
                     TYHAYAPYKLAAEVIYALV"
     gene            complement(350..835)
                     /locus_tag="VHM69_10970"
     CDS             complement(350..835)
                     /locus_tag="VHM69_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2740959.1"
                     /translation="MTLARRFIEEVHNGRDLDVVDELCHPDHTFHHWAIPEEQSGGLD
                     VVKRMVSLFYEISPDFMITITDEIVREDKTASLWAGTGLLREDLRSAGSPEEQINLTG
                     AYIHRIEDDKIIETSLVLDGYRNVQPGDQVRITRIDGEDLTDTEIKLGCCICPRCFWC
                     R"
     gene            complement(972..>4195)
                     /locus_tag="VHM69_10975"
     CDS             complement(972..>4195)
                     /locus_tag="VHM69_10975"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740960.1"
                     /translation="AGPLGGVDGLVGGNGFSPSSLVLLPAFVGWPFLPVFYSDRSYNF
                     RTFYHPYLNVFMRALNRDGVDGLLRRSIQVDSSSDYFASSYSPTELVGRSYPRDDVDF
                     SEGGAYAQYNWELFFHAPFMVADRLSKNQRFEEAQRWFHYVFDPTDTSALPTPQRFWR
                     TRPFYETTDEEYQKENIPNLLRLLATHGNPQVREDLEPEERARLEEIEAKVNRWRKNP
                     FNPHLIARMRTTAYQKTVVMKYIDNLIAWGDQLFRQDTMESINEATQLYVLAAELLGR
                     RPEEIPPRAVAEVHTYDTLEPRLDEFSNALVRIEEFVPLSNVPAPATTSEQSSLTLPT
                     MLYFCVPKNDKLLGYWDTVADRLFKIRHCMNIEGVVRKLDLFGQPIDPALLVRAAASG
                     VDIGSALSDVNAALPHYRFNVMAQKANELCSELKGLGSALLSALEKRDAEALALLRSS
                     QEIKVLDAARQVKERQIDEANEALEGLKRSREVVEIRRNYYDTREFMSLWESTQIGLM
                     AASLVLRGTEIGALILSGGLHLIPNAKIGAPTSLGVTYGGDNVANSAKSFGASLGEVA
                     ALTDQSASMAATMGNHQRRMDDWKHQGQLAAKELEQIDKQIVAAEIRKAIAEQELKNH
                     DLQVENAEEVDAFMRDKYTNRELYNWMVGQISSVYFQSYQLAYDVAKRAERAYRHELG
                     LQDSSFVKFGYWDNLRKGLLAGERLHHDVKRMEVAYLEQNAREYEITKHVSLARLDPM
                     ALARFKETGECFVNVPEAAFDLDYAGHYMRRIKSVGVSIPCVTGPYTGVNCTLTLLKS
                     SVRHSTALTGGNNNGRYAREEVEDSRFTDSVGAIQSIVTSSGQNDAGMFEPNLRDERY
                     LPFEGAGAISEWRLELPKEFRQFDYDTISDVVLHLRYTAREGGDRLKQQAISELEAAI
                     NEAALSEGKRGLANLFSAKHEFSSEWHRFLGATGKQELKLSLTQDRYPYVFSGRTITV
                     DRVDLLVRLSEGFDVADVGTDIKFRLKISDEPDSEPVKYKLPAEPNLGEALHILAEEE
                     EDIGGSPGEWTLTVGDPDSNPPQPRLNPDAVEDIGVVVHYTVA"
BASE COUNT          709 a         1278 c         1307 g          901 t
ORIGIN      
        1 gaccagcgca taaatgactt cggccgcgag cttgtaggga gcgtaagcat ggtacgtcgc
       61 gtaggccacc ggcatcgcgg ctccgtaggt cccgacaacg aggacggccg ctacgaccca
      121 cgctattcgt gcggttggta gccagcggcc atactgtgtg atctgcactc tggtgacacg
      181 gggcttcatg cctctaaatg tgaatctgtc gaaggttcgc ctcgacttcc gcatgatccg
      241 ggcgcgttcg caagatggat gagccgctcc tttctaccgg gtcccgagat aggggattga
      301 ccatcatcct gcgctctcgg tcaggtttct gtggaccgga agcgttaggt catctgcacc
      361 aaaaacaacg aggacatata cagcaaccga gctttatttc ggtatctgtc aggtcttccc
      421 cgtcgatcct agtgatacgc acttggtcgc ctggctgtac gtttcggtag ccatccaata
      481 cgagcgacgt ttctatgatc ttgtcgtcct cgatgcgatg gatgtaagct cccgttaagt
      541 tgatctgctc ttcgggagaa ccggcgcttc tcaaatcttc cctaagtagt ccggtacccg
      601 cccataggct tgccgtcttg tcttctcgta ctatctcgtc cgtgatcgtt atcatgaagt
      661 cgggcgaaat ctcgtagaac aggctgacca ttctcttgac aacgtcgagc ccgccgcttt
      721 gctcttcggg gatcgcccaa tgatggaaag tgtggtcggg gtggcacagc tcgtctacca
      781 cgtccaagtc ccgtccgtta tgaacttcct cgatgaaacg cctcgcaagg gtcatgcgct
      841 attcctcctt ccggtcaatg agcgaatgtt cgtcgcctga ggcgacgaac attcgccatt
      901 ccgctcgctc ccctcgatcg tccggggatg aacaacgacg aaactatctc cgccgtgctg
      961 ccgcgcccgt ctcacgcgac cgtgtagtga acaacaacgc cgatgtcctc cacggcgtcg
     1021 gggttcaatc ttggttgcgg cgggttgctg tcgggatccc ctaccgtcaa ggtccactca
     1081 cccggactgc caccgatatc ctcttcctct tccgcgagga tgtgcagggc ttcgcccagg
     1141 ttcggctccg cgggaagttt gtatttgacc ggctcgctat ccggctcgtc ggagattttg
     1201 agcctgaact taatgtcggt gccaacatcc gcgacgtcga agccttcgga gagccgtacc
     1261 agcaagtcca cgcggtcaac cgtgatggtc cgaccgctga agacatacgg gtagcgatcc
     1321 tgcgtcaacg acagtttcag ctcctgcttt cccgtggcac ccaggaagcg gtgccactcg
     1381 ctcgagaact cgtgcttcgc gctgaacagg ttcgcaagcc ctcgcttgcc ttccgagagc
     1441 gcggcctcgt ttatggcggc ctctagctcg ctgatggcct gctgcttgag ccgatctccg
     1501 ccctcgcgcg cggtgtagcg gaggtgcagg accacgtcag agatggtgtc gtagtcgaac
     1561 tgccggaact ccttgggcag ctcgaggcgc cactcgctga tggcgccggc gccctcgaag
     1621 ggcaagtagc gctcgtcgcg caggttcggt tcgaacatgc cggcgtcgtt ctggccgctg
     1681 ctggtgacga tggactggat ggcgccgacg ctgtcggtga agcgcgagtc ctcgacttcc
     1741 tcgcgcgcgt acctgccgtt gttgttgccg cctgtcagcg cggtgctgtg acggaccgag
     1801 ctcttgagca gggtgagcgt gcagttgacg ccggtgtaag ggccggtgac gcacgggatg
     1861 ctcacgccga ccgactttat gcgccgcatg tagtgtcccg cgtagtccag atcgaaggcc
     1921 gcctcgggca cgtttacgaa gcattcgccg gtctccttga agcgcgcgag cgccatcggg
     1981 tcgagccgcg cgagcgagac gtgtttggtg atctcgtact cccgcgcgtt ctgctcgagg
     2041 tacgctacct ccatgcgctt gacgtcgtgg tggagtcgct cgcccgcgag aaggcccttc
     2101 ctgaggttgt cccagtagcc aaacttgacg aagctagagt cctgcagtcc cagctcgtgg
     2161 cggtaagccc tctcggcgcg cttggcgacg tcgtaggcga gctggtagct ctggaagtag
     2221 acactggaga tctggcccac catccagttg tagagctcgc ggttggtgta cttgtcgcgc
     2281 atgaaagcgt cgacttcctc ggcgttctcg acctgcaggt cgtggttctt cagctcctgc
     2341 tcggcgatag ccttgcggat ctccgcggcg acgatctgct tgtcgatctg ttccagttcc
     2401 ttggcagcca gctgaccctg gtgtttccag tcatccatgc gacgctggtg gttccccatc
     2461 gtggcagcca tcgacgcgct ttggtccgtg agcgccgcca cctctccgag cgaagcgccg
     2521 aagctcttcg cggaatttgc gacgttgtct ccgccgtagg tgacgcccaa ggacgtcgga
     2581 gccccaatct tggcgttcgg gatcaaatgc aatccaccag acaagatgag cgcgcctatc
     2641 tcggtccctc tgaggaccaa actggccgcc atcaggccga tctgggtcga ctcccagagg
     2701 ctcatgaact cgcgcgtatc gtagtagttc ctgcggatct ctacgacctc acgtgatcgc
     2761 ttgagtcctt cgagagcctc gttggcttcg tcgatctgcc tctccttgac ctgtcgagcg
     2821 gcgtctagca ccttgatctc ttgggaggag cgcaagagcg caagcgcctc ggcgtcgcgc
     2881 ttttcgaggg cggagaggag ggcggagccc aggcccttga gttcggagca gagctcgttg
     2941 gccttctgtg ccatcacgtt gaatcgatag tgcggcagcg cggcgttgac gtcgctcaac
     3001 gcgctgccga tgtcgacgcc ggacgccgcg gccctgacca ggagcgcggg gtcgatgggc
     3061 tgtccgaaga ggtccagctt gcgcaccacg ccctcgatgt tcatgcagtg gcggatcttg
     3121 aagagcctgt ccgccaccgt gtcccagtag ccgagcagct tgtcgttctt gggcacgcag
     3181 aaatagagca tggtcggcag cgtcagggac gactgctctg aggtggtcgc cggtgcggga
     3241 acgttggaga ggggcacgaa ctcctcgatc cgcaccagcg cgttggagaa ctcgtcgagc
     3301 ctgggctcga gggtgtcgta ggtgtgcacc tcggcgaccg cgcgcggcgg tatctcttca
     3361 gggcgcctgc ccagaagctc cgcggccagc acgtagagct gggtcgcctc gtttatcgac
     3421 tccatcgtgt cctgccggaa gagctggtcg ccccaggcga tgaggttgtc tatgtacttc
     3481 ataaccaccg tcttctggta agcggtggtc cgcattcggg cgatcaggtg tgggttgaac
     3541 gggttcttgc gccacctgtt gaccttcgcc tcgatctcct cgagccgcgc gcgctcctcc
     3601 ggttcgaggt cctcgcgcac ctgcgggttc ccgtgcgtag ccagaagccg caacaggttg
     3661 gggatgtttt ccttctggta ttcctcgtcg gtagtctcgt agaagggcct ggtgcgccag
     3721 aagcgttgcg gcgtcggaag ggcggaggtg tccgtgggat cgaagacgta gtggaaccat
     3781 ctctgcgcct cttcgaagcg ctggttcttg ctcaggcggt cggcgaccat gaagggggcg
     3841 tggaagaaca gctcccagtt gtactgggcg tatgccccgc cctcggagaa gtccacgtcg
     3901 tcgcgggggt aggatcgccc cacgagctcc gtgggagagt agctggaggc gaagtaatcg
     3961 ctgctggaat cgacctgtat cgagcgccgg agcaggccgt cgacgccatc gcggttcagc
     4021 gcccgcatga acacgttcag gtaggggtgg tagaacgtgc ggaaattgta gctccgatcg
     4081 ctgtagaaca ccggcaggaa cggccacccg acgaacgcgg gcaacagcac caggctactt
     4141 ggggagaagc cgttcccccc aacgagcccg tctacgccac cgagtgggcc cgcga
//
LOCUS       DATAIR010000451         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_28363, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000451 DATAIR010000000
VERSION     DATAIR010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28363"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1204
                     /locus_tag="VHM69_10980"
     CDS             347..1204
                     /locus_tag="VHM69_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2740961.1"
                     /translation="MILNPSAGKGRAGDRRAFVSRFLEERGVRPIWHLTEGPGHAGSI
                     VRELPEDVLVVAVGGDGTVHEVAAACATTGRTMGVLPAGSGNDYVKALGVGTGLRRAL
                     EILVGGKTRVVDMGEVNGVPFNNGLGIGFDAEVAAGVAEAPVYLGGTGRYLWSVGRLL
                     RDFRCHEARLVLDGEVVESKTILVAVAIGTTYGSLFRLTPEAVLDDGLFDVIWSDEVN
                     RAEVLRLIPAALRGTLSKRPKVHTARAREVEIELSEEVPAHVDGEMLAPTRNFRARVL
                     PGALRVVAQ"
     gene            1245..1556
                     /locus_tag="VHM69_10985"
     CDS             1245..1556
                     /locus_tag="VHM69_10985"
                     /inference="COORDINATES: protein motif:HMM:NF039323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6019 family protein"
                     /protein_id="HEX2740962.1"
                     /translation="MPDLGSRNALIGAVVFALLVVFLSISGVQPFGLLFYGGLYVILP
                     LIAMYFIVRWAVSAGIRDASKRGFGQNPRTPLDILDERYARGEIGRDEYEQVRRALET
                     G"
     gene            1671..>2350
                     /locus_tag="VHM69_10990"
     CDS             1671..>2350
                     /locus_tag="VHM69_10990"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2740963.1"
                     /translation="MRAFVFPGQGGGVSEPEPEMVPEIRRVVGERIPDQVKIFARAAR
                     DADESRILEVRPDVVAGHSLGEYGAAYAAGCFDFETGLWLVAQRDHFLAEAAKECPGG
                     MVAILRTDPAEVEKALGGPGGPVVANYNSPRQTVVSGLRDALGDAVSKIRGRKIPLNV
                     SFAAHSPYVAAAGEKMRGVLDSVEFRVPQVPIVSAMDGAVLTSADAVKEALKGQMVAP
                     VRWVKVVET"
BASE COUNT          418 a          666 c          795 g          471 t
ORIGIN      
        1 cctgcgcgac tctcacccaa tgaacgataa tgctctatac ctgcacttta gctctatatg
       61 tatcagaagt ctatggtagt ggagaacctt cttgtgactt cccgcagata ttccggctca
      121 ggtcgtgctg cgtttttccc gcaaaggagc ctctgttcgc ttcggaccgc ggacacggtt
      181 gggcgacggg catcccgttc cctggtagct tatgctgtct ggcgaacaga tcatcattct
      241 cgccatagtc tatacctcgc gtagagcctg aatgatctcc gaccgggccg ctgaagtgta
      301 gttctgttat tttccggctg tgatgttcgg tggaggagag gcgcatttga tcctcaaccc
      361 gtcggccggc aaggggcggg cgggggatcg gcgggcgttc gtctcgcggt ttctggagga
      421 gcggggggtg cgcccgatct ggcaccttac ggaaggaccc gggcatgctg gcagcatcgt
      481 acgcgagttg cctgaggacg ttctagtggt ggcggtcggc ggagacggga cggtgcacga
      541 ggtggctgcg gcatgcgcca cgacgggaag gaccatgggt gtgttgcccg ccggcagcgg
      601 caacgattac gtcaaggcgc taggggttgg cacgggactc cggcgggcgc tggaaattct
      661 cgtcggagga aaaactcgcg tcgtcgatat gggagaggtc aacggggtcc ccttcaacaa
      721 cgggctcggc atcggtttcg acgccgaggt ggctgcgggc gtcgctgagg cgcctgtgta
      781 cctcgggggg acgggaaggt acctgtggtc ggtggggcgg ctgctacggg atttccggtg
      841 ccacgaagcg aggctggtgc tcgacggcga agtcgtcgag agcaaaacca tactagtcgc
      901 ggtcgccata ggcaccacgt acggctcgct gttccggctc acgcccgagg ccgtactaga
      961 tgacggttta ttcgatgtga tctggtcaga tgaagtaaac cgggccgagg tattgcggct
     1021 cataccggcg gctctccggg gaaccctctc gaagcgtccg aaggtgcata cggcccgcgc
     1081 tagggaggtc gaaatagagc tctccgaaga agtccctgcg cacgtagacg gcgagatgct
     1141 cgcgccgacc cgcaacttcc gggcgcgggt tctgccagga gcattgcgcg tcgtggcaca
     1201 gtagccagta gaatgcgtgt acgaaaatcg gaaggagggt gtccttgcct gacctggggt
     1261 ccagaaatgc gttgatagga gccgtcgtct ttgcgctact ggtcgtcttc ctttctatct
     1321 caggcgttca accttttggg ttactgttct acggcggctt gtatgtgatc ctgccgctta
     1381 tcgcgatgta ctttatcgtg cggtgggccg tgagtgccgg catccgcgac gcgagcaaga
     1441 ggggcttcgg acaaaatcct cgcacaccgc tcgatattct agacgagcgc tacgctcgag
     1501 gtgagatcgg ccgggacgag tatgaacagg tgcgtcgggc cctcgaaacg gggtaggcga
     1561 cactccgtgc aaagctctac taaagagtgc attcttgtgg tacaaacatt tctctacacc
     1621 attggggtag ggtcgtattt cctagttata tagaggtctg gcgaacaaag atgagagcgt
     1681 ttgtatttcc aggacagggc ggcggggtat ctgaacctga gccggagatg gtgccggaga
     1741 tccggcgggt cgtcggcgag cgcatccccg atcaggtcaa gatcttcgcc cgcgcggccc
     1801 gcgacgccga cgagagccgc attctggagg tgcgtcccga cgtggtagcc gggcactccc
     1861 tcggggagta cggtgctgcc tatgcggccg gttgcttcga cttcgagacc gggctctggc
     1921 tcgtggcgca gcgcgaccac ttcctcgcag aggccgccaa agagtgtccg ggcgggatgg
     1981 tcgccatcct caggacggac cccgccgagg tcgagaaggc gctcggcgga ccaggcggcc
     2041 ctgtggtcgc caactacaac tccccgcgcc agacggtcgt ctcagggctg cgagacgccc
     2101 ttggggacgc ggtatcgaag atccgtggcc gcaagatccc tctcaacgtc tccttcgccg
     2161 cccactcccc gtacgtggcg gcggcgggcg agaagatgcg cggcgtcctc gattccgtcg
     2221 agttccgcgt accgcaggtc cccatagtca gcgccatgga cggtgcggtg ctcacgagcg
     2281 ccgacgccgt caaggaggcg ctcaagggac agatggtcgc ccctgtgcgc tgggtcaagg
     2341 tcgtcgaaac
//
LOCUS       DATAIR010000452         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_151868, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000452 DATAIR010000000
VERSION     DATAIR010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151868"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="selA"
                     /locus_tag="VHM69_10995"
     CDS             <1..858
                     /gene="selA"
                     /locus_tag="VHM69_10995"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX2740964.1"
                     /translation="PEVIVSRGQLIEIGGGFRIPEVLALSGARLREVGTTNRTRLSDY
                     EDALNEKTRAILWVHPSNFETVGFTESVGVADLTTLGQPVVADIGSGALLPVGGEPRV
                     QDAVRDGAALALFSGDKLLGGPQAGIAVGSSRWISAMRRHPLARALRADKLCLAALEA
                     TLTSYLEGSAEQNLPTLGMLRTPAQEVEARARNLAEELAHNAPELEVDVVPSVARSGG
                     GTLPMHEIPSFAVRLGGADPDLLAEGLRACDPPVVGRVREGRLWLDVRTLLPGDEEAV
                     IGAVRDSNG"
     gene            851..2695
                     /gene="selB"
                     /locus_tag="VHM69_11000"
     CDS             851..2695
                     /gene="selB"
                     /locus_tag="VHM69_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX2740965.1"
                     /translation="MGDAGEHRIALTIGTAGHVDHGKTTLVRYMTGTDTDRLEEEHRR
                     GISIVPGYAELELPSGRRASLVDVPGHERFVKNMVSGSTGVDAFLLVVASDDGVMPQT
                     MEHLDVLRILGVEQGVVALSKIDAVDEEIVELAALEVEDLLDSRGMSAPIVRVSGTTG
                     EGVENLLLALDELSRDTADRRRDDLARLPVDRSFVLKGIGVVVTGTLWSGEIRPGDVL
                     HTPAGLRPRVRGVQNHGHPAEVAYAGARTALDLTGVEASQIEAGDVLLSHPVPASREL
                     DARVRLLESAKPLAHGVRARLHHGTRSTNARIRLTDGEELLPGMSSLARLRLDEPFVT
                     LPGDRFVLRSMDGITVGGGTVLDPAATGRRPDPSWLEALESADATKAIPLALARKPRE
                     GMTTEELSLVLGIPKDEVSRAVETTAGVETVGEIHTVADEISAARDHLLEALERRATE
                     RPESPELTVAEARTATGLSTLLADALLSEIETISVTDAGVTLPRGGVPEELEREAEML
                     LQELQGAGMEPPAAETTPALRLLVKRGDAVELGGSLFAARSAADSVLERIKTICREEG
                     EISLAGLRDSLSTSRKYAQAWLEYSDTSGVTSRTGDVRVLTRRHRQAM"
     gene            2722..2810
                     /locus_tag="VHM69_11005"
     tRNA            2722..2810
                     /locus_tag="VHM69_11005"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2754..2756,aa:Sec,seq:tca)
BASE COUNT          495 a          929 c          985 g          459 t
ORIGIN      
        1 cctgaggtga tcgtctctcg cggccagctc atcgagatcg ggggcggatt ccgcattccg
       61 gaggtacttg ctctctccgg cgcgcgcctc agggaggtcg ggacgacgaa ccgtacaagg
      121 ctctcggatt acgaggacgc cctaaacgag aagacccgcg cgatcctctg ggtccatcca
      181 agtaacttcg agacggttgg ttttacggag tcggtagggg tcgcagacct caccactcta
      241 ggtcagcctg tcgtcgccga catcggcagc ggcgcgttgt tgcccgtggg cggcgaaccg
      301 cgggttcagg acgccgtcag ggacggcgcg gctctcgccc tgttctccgg cgacaagcta
      361 ctcggcggac cgcaggcggg gatagccgtg gggagttcgc gttggatctc cgccatgcgc
      421 cgtcatcccc tcgcacgcgc cctccgcgca gacaaactct gcctcgcagc cctcgaggcg
      481 accctgacct cctacctgga aggcagcgcc gagcagaacc ttccgaccct gggaatgttg
      541 cggacgccgg cccaagaggt ggaagccaga gcccgtaatc tcgcggaaga gctagcgcac
      601 aacgccccag agctggaggt agatgttgtg ccttcagtag cccgcagcgg cgggggtacg
      661 cttccgatgc acgagatccc ctccttcgcc gtgcgacttg gtggcgcgga tccggacctg
      721 ctggccgagg gtctgcgcgc ttgtgacccg ccggtcgtcg gtcgcgtgcg tgaggggagg
      781 ttgtggctgg acgtgcgcac gctcctgcct ggtgacgagg aggctgtgat cggggccgtt
      841 agagactcga atgggtgatg cgggcgagca caggatagca cttaccatcg ggacggcggg
      901 gcacgttgac cacggcaaga cgacgctcgt ccgctacatg acgggcacgg atacagaccg
      961 cctcgaggag gagcaccgcc ggggcatctc catcgtgcct ggttacgccg agctggagct
     1021 cccgagcggc cggagggcga gcctggtgga cgtgcctggg cacgagcgct tcgtcaagaa
     1081 catggtctcg ggatcgaccg gggtcgacgc cttcctgctc gttgtcgcat ccgacgatgg
     1141 ggtgatgccc cagacgatgg aacaccttga cgtcctgcgg attctcggcg tcgagcaggg
     1201 cgtcgtggcc ctctcgaaga tcgacgcggt ggatgaagag atcgtggagc tggccgcgct
     1261 ggaggtggag gacctgctcg actcaagagg catgagcgct ccaattgtcc gtgtgagcgg
     1321 cacgacagga gagggtgtgg agaacttgct gctggccctc gacgagctgt ccagggatac
     1381 ggcggaccgg cggcgcgacg atctggcacg cctgcccgtg gaccgctcct tcgtgctcaa
     1441 gggtatcggg gtcgtcgtta cggggacgtt gtggagtggg gagatccgtc ctggtgacgt
     1501 cctgcacact cctgcgggcc tgcgcccgcg cgttcgcggc gtgcagaacc acgggcaccc
     1561 ggccgaggtc gcctacgccg gggcgcgcac ggccctggat ctaaccggag tcgaagcctc
     1621 ccagatcgaa gctggcgacg tcctgctctc acatcccgtc cctgcgagcc gcgaactgga
     1681 cgcccgagtc cgcctcctgg agagcgcgaa accgctcgca cacggcgtcc gcgcacggct
     1741 gcaccacggg acgcgctcca cgaacgcccg catacgtttg acggacggcg aggagctcct
     1801 gccgggcatg agctccctcg cccgtctgag gctcgacgag ccgttcgtca ccctccctgg
     1861 cgaccgcttc gtgctgcgct cgatggacgg gatcacggtg ggcggaggca ccgttctcga
     1921 ccccgccgca acgggacggc gccccgaccc gtcgtggctc gaagccctcg aaagcgcaga
     1981 cgcgacgaag gcaatccccc tcgcgctggc aaggaaaccc cgtgaaggca tgacgacaga
     2041 agagctctcg ctggtcctcg gcatcccgaa ggacgaggtt tcgcgcgccg tggagaccac
     2101 cgccggggta gagactgtcg gggagatcca caccgtagcc gacgagatct cggccgcaag
     2161 agaccatctc ctcgaagcgc tggaacgtcg ggccacagag cgacctgaga gccccgagct
     2221 caccgtcgcc gaggcccgca ccgcgacggg actgtcgaca ctgctcgctg acgccctgct
     2281 ttcggagata gaaaccatca gcgtgaccga tgccggcgtc accctgccgc gcggtggagt
     2341 gcccgaggag ctggagcgcg aggcggagat gttactgcaa gagttgcagg gcgccggcat
     2401 ggagcctcct gcggcggaga ctactccggc gttgcgtctg ctcgtgaagc gtggagatgc
     2461 ggtggaacta ggaggctcgc tcttcgccgc gcgctcggcc gccgactcgg tattggaaag
     2521 gataaagacc atctgcaggg aggaaggtga gatctcactt gccggtctcc gagacagcct
     2581 cagcacaagc cgcaagtacg cccaggcctg gctggagtac tcggacacat cgggcgtcac
     2641 cagccgcaca ggcgacgtcc gcgtcctcac ccgccgccac aggcaggcga tgtaataaac
     2701 tctgccaggt tccctaaacc cggaatgcgt gggcctggtg gcccggccgg tcttcaaaac
     2761 cggtttgcgg ccgtgatgcg gccgtggcgg ttcgattccc ccgcattccg cgattggatg
     2821 ctacaggcat cagggagaca tgcgcaggaa ctcaccccgg tcgtcgag
//
LOCUS       DATAIR010000453         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_398024, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000453 DATAIR010000000
VERSION     DATAIR010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398024"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHM69_11010"
     CDS             <1..183
                     /locus_tag="VHM69_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740966.1"
                     /translation="VGSRLMQVFCARMDAAREDAYLETDKQINVRFYQRFGFEVVGEE
                     DVLGVPNWFMRRTRRG"
     gene            254..820
                     /locus_tag="VHM69_11015"
     CDS             254..820
                     /locus_tag="VHM69_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2740967.1"
                     /translation="MVYSRDLNGEQANLPEEDVDVRGGLVLPPGVEEVFREFRTCEFS
                     TLARDGTPITWPLVTLWQPEEERFVLTTSIGLPRKALNVRRDGRVSLLFSDPTASGLE
                     DPPSVLVQGDAEVPDEIKTSPNHLREYWKRLFRIQPAGKMYGANALTRYFTDWYYMRL
                     YIYVRPRRIFVWPGGDFTSAPLEVDHVD"
     gene            810..1241
                     /locus_tag="VHM69_11020"
     CDS             810..1241
                     /locus_tag="VHM69_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740968.1"
                     /translation="MWTEIEKNLPQFETAVLNVPDQDGYPYSVRCRAEQDRRAGVLRL
                     DLAEAVNVQPGPASLLCHNHDEELWNQKIFLLRGRIEGVGDERVFRPEKFIPSIGTGG
                     ATGVVRMLLGARRATTAYLKKRGLARPTIPWAEIEAVKKEA"
     gene            1395..1973
                     /locus_tag="VHM69_11025"
     CDS             1395..1973
                     /locus_tag="VHM69_11025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2740969.1"
                     /translation="MARKGEILENPVTGQRIIFRQTSEDTDGALLEVESVYTKPTPTR
                     PPVHYHPGQEERFEVLAGDLHVVIGGEGRTLGAGETLLVPPGTTHSMWAEAEGVRVNW
                     QTRPALRTEAFFETISGISRDAKSGGKGSPNLLQAAVITQAYADEFRLARPPWAVQRV
                     LFAVLAPLGRLFGYSYPEGALSGVPSGSPRRA"
     gene            complement(1936..>2609)
                     /locus_tag="VHM69_11030"
     CDS             complement(1936..>2609)
                     /locus_tag="VHM69_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2740970.1"
                     /translation="MLAGAGANVVVADLKGEAPQGGRFVETDVTDEQSVQAAVDAVLE
                     LGDLRGAVNCAGIASAEKVLGKGGPHSLDSFAKVVQVNLVGTFNVVRLAAEAMVSNDP
                     LENGERGVIVNTASVAAFDGQIGQVAYAASKGGVVSLTLPVARELATSGIRVMAIAPG
                     IFDTPMMAGLPEDARVSLGNQVPFPSRLGKPEEYADLVRHIVENEMLNGEVIRLDGAI
                     RMAPR"
BASE COUNT          515 a          770 c          868 g          456 t
ORIGIN      
        1 gtgggaagcc gcctgatgca ggtcttctgc gccaggatgg acgcggcgcg cgaggacgcc
       61 tacctggaga ccgacaagca gatcaacgtc cgcttctacc agcggttcgg gttcgaggtg
      121 gttggcgagg aggacgtgct cggcgtcccc aactggttta tgcggaggac cagaaggggg
      181 tgagcaggtg gagatagggt cacagggaga cggacgagag acctggatga aggtagcgag
      241 acaggagaaa tctatggttt acagccgtga cttgaacggg gagcaggcga accttcctga
      301 ggaggatgtt gacgtgcgcg gaggactcgt tctcccgcct ggggtggagg aggtgttcag
      361 ggagttccgc acctgcgagt tctcgacgct tgcccgtgac ggaaccccga tcacctggcc
      421 gctggttacc ctgtggcagc cggaggagga gcgtttcgtt ctcacgactt cgatcggcct
      481 accccgtaag gccctcaatg tgcggcgcga cgggagggtc tcgttgctct tctccgaccc
      541 tacggcgagc ggcctcgaag atcctccgtc agtactggtg cagggcgatg ccgaggttcc
      601 tgatgagatc aagaccagcc ccaatcatct cagggagtac tggaagaggc tattcaggat
      661 ccaacccgcg ggcaagatgt acggtgccaa tgcgctcacg cgctacttca ccgactggta
      721 ctacatgagg ctctacatct acgtcaggcc acggcggatc ttcgtctggc cagggggaga
      781 ttttacgagc gcgccactgg aggtggacca tgtggactga gatagagaag aacctgccgc
      841 agttcgagac cgccgtgctc aacgtccctg accaggatgg atacccgtac agcgtccgct
      901 gcagggcgga acaggatcgc cgggcagggg tccttcgact cgatctcgca gaggcagtaa
      961 acgtccaacc cggaccggcc agcctactct gccacaacca cgatgaagag ttatggaacc
     1021 agaaaatctt cctcctcagg ggaaggatag aaggagtggg ggacgagcgg gtcttcaggc
     1081 cggagaagtt catcccgagc atcgggacag ggggcgcaac cggtgtggta cgtatgttgc
     1141 tgggagcacg tagagctact acggcgtact tgaagaaacg cggcctcgcc cgtccgacca
     1201 tcccctgggc cgagatcgag gcggtcaaga aagaggcctg acggtaagag gatagccttc
     1261 gtcagcaacc tgacggcgac cgggcgctgg acttccaacc ttcctggcaa ctactccctg
     1321 gcgccggcag tcgtagtcac gggcgggact acccgccccg tatggcggat aatgacgaac
     1381 gaaggaggca cggcatggca aggaaaggcg agatactcga aaaccctgta acgggacagc
     1441 gtattatctt tcgccagacg tccgaagaca cggacggcgc gctcctcgag gtcgagtcgg
     1501 tgtacacgaa gccgacccca acgaggccgc cagtccacta ccacccgggc caggaggagc
     1561 gtttcgaggt cctcgctggt gatctgcacg tcgtgatcgg cggcgagggg aggacgctgg
     1621 gagcgggcga gacccttctt gtaccacctg gaaccacgca ctcgatgtgg gcggaagcgg
     1681 aaggggtgcg cgtcaactgg cagacgcggc cggcgctaag gacggaggct ttcttcgaga
     1741 ctatatctgg gatctcgcgg gacgcgaagt ccggcgggaa aggatctcca aacctcttgc
     1801 aggccgctgt gatcacacag gcttacgcgg acgagttccg cctcgccaga ccaccctggg
     1861 cggtgcagag ggtgctcttt gcggtgctcg caccgctagg caggttgttt ggctacagct
     1921 accccgaggg cgctctcagc ggggtgccat ccggatcgcc ccgtcgagcc tgatgacctc
     1981 gccgttgagc atctcgttct cgacgatgtg ccgcacgaga tccgcgtact cctccggctt
     2041 tccgagacgg gaggggaagg gtacctgatt gcctagtgag acgcgggcgt cttcgggcag
     2101 acctgccatc atcggggtat cgaagatgcc tggcgcgatc gccatgacgc ggatgccgct
     2161 cgtggcaagc tcgcgggcga cagggagggt cagagagacg acgccacctt tagaggcggc
     2221 gtaggctacc tgcccgatct ggccgtcgaa cgcggccacg ctggccgtgt tgacgataac
     2281 tccgcgctct ccattctcta atggatcgtt gctgaccatc gcctcggcag cgagcctgac
     2341 gacgttgaag gtgccaacga ggttgacctg cacgaccttc gcgaacgaat caagcgagtg
     2401 cggacctcct tttccgagga ccttctccgc gctcgctatg cccgcgcagt ttacggcgcc
     2461 gcgcaaatcc cccagctcga ggacggcgtc aacggcagct tgcacgctct gttcgtcggt
     2521 gacgtcggtc tcgacgaagc ggcctccctg gggcgcttcg cccttcaggt cagcgactac
     2581 gacgttggcg ccggcaccgg cgagcatgc
//
LOCUS       DATAIR010000454         4971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_197636, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000454 DATAIR010000000
VERSION     DATAIR010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197636"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VHM69_11035"
     CDS             complement(<1..879)
                     /locus_tag="VHM69_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2740971.1"
                     /translation="MEERVPHATEHPDLKRGIGPYLLLFFVLGDIVGAGIYALVGEVG
                     AVTGGAIWSAFLFAFVLAVFTAASYAELVTKYPRAGGSATYVNNAFRSPFVSFMVAFA
                     VATSSITSACVLTLAFSGDYLSQFVGIPSLVAAVIFLIVIGCINFYGISESVRINVVL
                     TCIEIFGLLLIVLIGLTALGSGTAEVGRAFEFKEGGSVLTALLAGAVLAFYALIGFDD
                     SVNVAEEVRRPNRIYPRAIFGGLLIAGFVYLLVTITAAMVVPISELSASDGPLLEVVE
                     RGALPIPTKFFAAIALL"
     gene            1076..1456
                     /locus_tag="VHM69_11040"
     CDS             1076..1456
                     /locus_tag="VHM69_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2740972.1"
                     /translation="MQLDLVGIVVRDMRASLEFYRRLGLDIPEGAEDEPHAEATTPSG
                     LRVAWDTAELIQQIDPTWTEPSGGHRIALAFLCSSPAEVDAKYDELAALGHGHREPWD
                     AFWGQRYAIVKDPDGNAIDLFAPL"
     gene            complement(1474..2562)
                     /locus_tag="VHM69_11045"
     CDS             complement(1474..2562)
                     /locus_tag="VHM69_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2740973.1"
                     /translation="MTDKIVFTRGVPPPEAFPTEELGACLDAVVREDASVVLQYGQQP
                     GYAPLREELAKEYGVSPAEILVGNGSLQLQDLVSSYLIQPGTAVFTEQPSYDRAIKTF
                     RRRGARAVGIPLEEDGISIERLEAALGREVPSFLYLVPDFQNPAGATLSLDKRRRVVE
                     LADHYGFWVIEDIPYRKLRYEGDDLPMLREIGSPRVITMSSFSKLLSPGIRVGFMISP
                     EELVREVTALGEDTYLSPVLPTQAAVAEYLRRGLLGPNIERLKDLYTPRWKAMAGAVR
                     SELPGAQAFIPSGGFFVSVMLPDDANTENLVGRAKDIGLVLTPGEAFFADPDEGGEAD
                     GERFVRLPFCAVTPEQIEEGVSRLAGLL"
     gene            complement(2647..>4971)
                     /gene="dnaE"
                     /locus_tag="VHM69_11050"
     CDS             complement(2647..>4971)
                     /gene="dnaE"
                     /locus_tag="VHM69_11050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2740974.1"
                     /translation="DVDIDFSVSGRSEVMRYVTDKYGGHEHVAQIITFGTIGAKAAIR
                     DSGRIYQYPYSDTDKLAKFIPDKPVGTTLRDVLRHENGSYVAGEKHPGAAREIIQFVQ
                     QDEAAKQVLDTAFEIEGFARHAGTHAAGVVISEEPLTDIVPLQAVKKASVKSDPMAGE
                     EEEQLAVMVQHPMSDVEALGLLKVDFLGLRNLDVIEETLETIRRSGGEEPDIRTIPLD
                     DEKTLKLFARGDTFGVFQFESSGMQRMLQEVRPDRFDDLVALNALYRPGPMDYIPNFK
                     RGKHDPESVTYLDERLKPILEPTYGVAAYQEQLMEISKTLGGFTPGEADTLRKAIGKK
                     NAAMLATLKDKFMAGCASSGVSTEGAEELWNWMEKAGGYSFNKSHSACYSFLAFQTAY
                     LKAHYPEAYMAALMSSVMNTKDRVPQYVAEARAMKIEVLPPDVNESGRRFTAVGETIR
                     FGLSAVKNVGDSCVDAIIAAREEGGPFEDIFDFCERVDFKTYNKRTLESLIKCGAFDN
                     VGPSRAAMVAVHAKAVERSTKGLKGASEDQFSMFEASELTPPKPEFPDVEEDHRQSLE
                     WEKETMGLYVSDHPLRPVLHKLRKHIDTTVSELEGYRDGAVVWVGGLATSVRTNTTRK
                     GDMMAMLQLDDTRGLAEVMVFPRVYAKYAASVREDAVLKVKGKVEMKEGIPRIVALEM
                     EELHLEPGPDPVYLDAGVFVGLSRKVAREAFELLLRHPGQSPVFLVSGDGMLEEKIGS
                     IGDSSDLHAELKQLLGPRCLTYTPPAEPQMEQVS"
BASE COUNT          917 a         1603 c         1516 g          935 t
ORIGIN      
        1 gaggagcgcg atggcggcga agaacttggt cgggataggc agagcccccc tctcgaccac
       61 ctcgagcaga ggcccgtccg acgccgagag ctcagatatc gggactacca tcgcggcggt
      121 gatagtgacg agcagataga cgaatccggc gatcagcaac ccaccgaaga tggcgcgcgg
      181 atagatcctg ttcggcctgc gcacctcctc ggcaacgttg acggagtcgt cgaagccgat
      241 tagcgcgtag aaagccagga cggcgcccgc gaggagggcg gtgagcacgc taccgccctc
      301 tttgaactcg aaggcccggc cgacctccgc cgtccccgag ccgagcgcgg tgaggccgat
      361 caagacgatg aggagcagcc cgaagatctc tatgcaggtc aggaccacgt tgatcctcac
      421 cgactcggag atgccgtaga agttgatgca gccgatcacg atcaggaata tgaccgcggc
      481 gacgagcgaa ggtatgccga cgaactggct gaggtagtcg cccgagaagg cgagggtgag
      541 gacacaggcc gaggtgatgc tcgacgtcgc cacggcgaaa gcgaccatga agctgacgaa
      601 gggcgacctg aaggcgttgt tgacgtacgt ggctgaccca ccggcccggg gatacttggt
      661 tacaagctcg gcgtaggaag ccgcggtgaa aacggcgagc acgaaggcga acaggaacgc
      721 gctccagatg gcgccgccgg taaccgcccc gacctccccg accagcgcgt agatccctgc
      781 ccccacgatg tccccgagga cgaagaagag caacagataa ggacctatgc ctcgcttcag
      841 gtccgggtgt tcggtagcgt gaggtacacg ctcttccatc ggctccacct ccctcgccgc
      901 cggcctcagc cgtacgacct cccgttgcta tgccggatcc atgttacctg aataacgtgt
      961 gatcttcatt ggacatgcaa cgtgtctccg ttttcttcac accccatgtg aaatcgtccg
     1021 ttggcaaggc gtccccactc cagtacgctc cgaaggaccg aagaggagga cgaacgtgca
     1081 acttgacctc gttggcatcg tcgtgcgcga tatgcgcgcc tcgctggagt tctaccgcag
     1141 gctcggcctt gacatccccg agggcgccga ggacgaaccc cacgccgagg cgaccacacc
     1201 ctcgggcctg cgcgtggcgt gggacaccgc cgagcttatt cagcagatag atcccacctg
     1261 gactgagccc tcaggaggcc accgtatcgc cctggccttt ctgtgttcga gccccgccga
     1321 ggtagacgcg aagtacgatg agcttgccgc tcttggccac ggacacaggg agccatggga
     1381 cgcgttctgg ggccagcgct acgccatcgt gaaggacccc gacggcaatg cgatagacct
     1441 cttcgcgccc ctatagggag ccgtacgccc ccttcagagc aggccggcca gacggctcac
     1501 gccctcctcg atctgctctg gcgtcacggc gcagaagggc aggcgtacga acctctcgcc
     1561 gtctgcttct cctccttcgt ccgggtcggc gaagaacgcc tcgcccggcg tgagcacgag
     1621 ccctatgtcc ttcgcccggc ccacgaggtt ctccgtgttg gcatcgtccg ggagcatcac
     1681 gctgacgaag aacccgccgg aggggatgaa cgcctgggca ccgggaagct cgctgcgtac
     1741 cgcgccggcc atcgccttcc agcggggcgt gtacaggtcc ttcagacgct caatgttcgg
     1801 cccgaggagt ccgcgtctca agtactccgc gacggcggcc tgcgttggga gcacgggcga
     1861 gagatacgtg tcctcgccga gagccgtcac ctccctgacc agctcctccg gtgagatcat
     1921 aaaccccacc ctgatgccag gactcaggag cttgctgaac gagctcatcg tgatgacgcg
     1981 gggggagcct atctcccgca gcatcggaag gtcatcgccc tcgtagcgca gtttgcggta
     2041 cggaatatcc tcgataaccc agaagccgta gtgatccgcc aactccacga ccctccgccg
     2101 tttatcgagg gagagcgtcg cccctgcggg gttctgaaag tcaggcacga ggtaaaggaa
     2161 ggaaggcacc tcgcgcccca gcgccgcctc cagccgttcg atgcttatgc cgtcttcctc
     2221 caatgggata ccgacggcgc gggctccgcg gcgcctgaag gttttgatcg cccggtcgta
     2281 actaggctgc tccgtgaaga ccgcggtacc tggttggatc aggtacgacg aaacgaggtc
     2341 ctgcagctgt aacgagccgt tgccgaccag gatctcagcc ggagagacgc cgtactcctt
     2401 cgccaactcc tcgcgcagcg gggcgtatcc gggctgctga ccgtactgca acaccacgct
     2461 cgcatcctcg cgcaccaccg catccagaca cgcgccgagt tcttcggtcg ggaacgcctc
     2521 gggcggtggc accccacggg tgaaaactat cttatccgtc atgagcacaa gcctccggtt
     2581 ctagatcctg gaagcgtcaa tcatcgcccg gcctgcggcc aagcgcaacc ctacggcaac
     2641 ttgtagctaa gagacctgct ccatctgagg ctcggcggga ggagtgtagg tgaggcacct
     2701 gggtccgaga agttgtttga gctcggcgtg taggtcggat gaatctccga tgctcccgat
     2761 cttctcttcc agcatcccgt cgccggagac gaggaagacc gggctttgcc caggatggcg
     2821 cagcaacaac tcgaacgcct cccgcgccac cttgcgcgaa agacccacga atacgccagc
     2881 gtcgaggtat acgggatccg gcccaggctc caggtgcaac tcctccatct cgagagccac
     2941 gatgcgaggg atgccttctt tcatctcgac cttccccttg accttgagta cggcgtcctc
     3001 gcgtacgctt gcggcgtact tggcgtagac cctcgggaag accatgacct cggcgagtcc
     3061 acgcgtgtcg tcgagctgga gcatggccat catgtcgccc ttgcgtgtgg tgttggtccg
     3121 cacggaggtc gcgaggcccc cgacccagac cactgcgccg tccctatacc cttcgagctc
     3181 cgagacggtg gtatcgatat gcttcctgag cttgtgaagc acggggcgca ggggatggtc
     3241 ggagacgtag agacccatgg tctctttctc ccattccagg ctctggcgat ggtcttcttc
     3301 tacatcgggg aactcgggtt tgggcggcgt gagctctgac gcctcgaaca tcgagaactg
     3361 gtcctcgctc gctcctttga gacccttggt cgaccgctcg accgcctttg cgtggactgc
     3421 gaccatcgct gcccgcgagg gtccgacgtt gtcgaaggcg ccgcacttta tgagggattc
     3481 caacgtgcgc ttgttgtatg tcttgaagtc gacacgctca cagaagtcga agatgtcctc
     3541 gaacgggcca ccctcctcgc gggcggcgat gatcgcatcg acacaagagt cccctacgtt
     3601 cttgacggcc gatagcccga aacggatggt ctcccctacc gccgtgaagc ggcgcccgct
     3661 ctcgtttacg tcggggggaa ggacttctat cttcatggct cttgcctcgg cgacgtactg
     3721 cggcaccctg tccttggtgt tcatcacgct gctcattagg gcggccatgt acgcctcggg
     3781 gtagtgcgcc ttgaggtagg cggtctggaa agcgaggaac gagtagcagg ccgaatgact
     3841 cttgttgaaa gagtagccac cggccttctc catccagttc cacagctcct cggcgccctc
     3901 agtcgagacg ccgctggacg cacaaccggc catgaacttg tccttgagcg tggcgagcat
     3961 cgcagcgttc ttcttgccga tagccttgcg gagcgtatct gcttcgccag gcgtgaagcc
     4021 gcctaacgtt ttggagatct ccatgagctg ctcctggtag gccgcgacgc cgtaggtcgg
     4081 ctccaggata ggctttagtc gctcgtcgag gtacgtgacg ctctcggggt cgtgcttgcc
     4141 gcgcttgaag ttggggatgt agtccatcgg accgggcctg tacagggcgt tcagcgccac
     4201 aaggtcgtcg aaacggtcgg gcctgacctc ctggagcatc cgctgcatcc cgctcgactc
     4261 gaactggaag accccgaaag tgtcgcccct ggcgaagagc ttcaaagtct tctcgtcgtc
     4321 gagaggaatc gtacggatat cgggttcctc gccgccgctg cggcggatcg tttcgagggt
     4381 ctcttcgatc acatccaggt tcctgagtcc caggaaatcc accttgagca ggcccagcgc
     4441 ctccacgtcg ctcatgggat gctggaccat cacggcgagt tgctcctctt cctcacccgc
     4501 catgggatcg ctcttcacgc tggctttctt caccgcctgc aaaggaacta tgtcagttag
     4561 cggctcctcg gagattacga cgccggcggc gtgtgtcccc gcgtggcggg cgaacccctc
     4621 tatctcgaag gcggtatcga ggacctgctt cgccgcctcg tcctgctgta cgaactggat
     4681 gatctcgcgc gccgccccag gatgcttctc gccagcaacg taagaaccgt tctcgtggcg
     4741 caacacgtcc cgcagtgtgg ttcctacggg cttgtccggg atgaacttgg cgagcttgtc
     4801 ggtgtcagag tagggatact ggtagatgcg ccccgagtcc ctgatggccg ccttcgcgcc
     4861 gatagtcccg aacgtgatga tctgggctac gtgctcgtgg ccgccgtatt tgtccgtgac
     4921 gtagcgcatc acctcggagc gccccgagac cgagaagtct atgtccacgt c
//
LOCUS       DATAIR010000455         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444013, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000455 DATAIR010000000
VERSION     DATAIR010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444013"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1269
                     /locus_tag="VHM69_11055"
     CDS             70..1269
                     /locus_tag="VHM69_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2740975.1"
                     /translation="MIADVTRSLGTDYYLLDELFTDEERAIRDRVRAFADAEVIPIIN
                     EYWEKAEFPFELVPKVAALNIAGGPIEEYGCPGMSQVAAGLVAIEMARGDGSFNTFFG
                     VHSGLAMGTVAICGSEEQKRRWLPGMARMEKIGAWGLTEPDHGSDSVMLETTARRDGD
                     GWVLNGKKRWIGNATFADLVIIWARDVEDGQVKAFVLEKDENGEHHPGYSTELIKGKM
                     GKRAVWQPDIFLDDVRVPGENKLEGANSFKDTNKVLTATRSGVAWEAIGHAIAAYEAA
                     LQYAKERVQFGKPIASFQLIQNKLSNMLAEITNMQLMCFRLAQLQEQGKMTGPMASLA
                     KMNNARKARQVCADARDIMGGNGVLLEYHVARHLSDMEIVYTYEGTDTIQSLIVGRDV
                     TGISAFV"
     gene            complement(1284..1922)
                     /locus_tag="VHM69_11060"
     CDS             complement(1284..1922)
                     /locus_tag="VHM69_11060"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX2740976.1"
                     /translation="MSLRNAALAGMIGPAVFAFVAAVLTVAQYGFMVRLGWDPIGASD
                     VPWPSGLALGPFGWLQVLNFVFFGLTLIVFALGLHRGVASGSWISPALLVVAGIGLVL
                     AGFKTDPYLSEGPQTWHGWIHLLAFILLVLSLLLSLFFWWWRLRRDPRWRGYDLYTLI
                     TAILYLALLFVSVWQWGFYLFLAVILTWIEVMAIRLRSVAANASARRHPRVR"
     gene            2117..3133
                     /locus_tag="VHM69_11065"
     CDS             2117..3133
                     /locus_tag="VHM69_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2740977.1"
                     /translation="MKAIRLHEIEGPESLVYENIPEPEPGAGEIVVRLHAAALNRRDV
                     FVTRGQYPGARPDALPVILGSDGSGEVAARGDGADGPSEGTEVVINSALYWGDDPRVP
                     GKEYRILGLPDDGTFAQFVKVPADYVFPKPSHLSHEEAAAIPLGGQTAYRALVTRGQI
                     QNGETVVVPGIGSGVATFVVQMAAALGARVFVTSGSDEKIEKAKELGAEGGVNYNNED
                     WSKELKKMVGKVDLSVDHVGGEAFDALVSLAAPGGRIVTFGSTAGPKVSVVMPRIFLK
                     HLTVLGTAMGTPEEFGAMLDLYADHGLKPAINKSFPLEETASAMEYMEKGVGIGKIVL
                     DVPA"
     gene            3221..4174
                     /locus_tag="VHM69_11070"
     CDS             3221..4174
                     /locus_tag="VHM69_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2740978.1"
                     /translation="MRRFEVADLSPFLAYRNDPRVARYQDWDSYDEREALDFIRAMKS
                     SEPGSPGEWFQFAVVLKGTGEPIGDCGLKTEEDTRQAEIGVTFAREHQGKGYACEAVS
                     RLLDYAFSDLGMHRVVAITDRQNAPSVTLLERLGMRREGSFTRNVWFKGRWSSEYLYA
                     ILRDDWLRRHERDERVAQKLRIVSEPHGSPGDATFVREGLALFNVATTGDSYYSPLAI
                     YLKDERGAVLGGALGHVYGGWLDLEYLWVIEPFRGQGHGGKLLEAAEDEARAQGCHGV
                     FLTTFSFQARPFYQNFGYEVVAEIPEYPRGHSRYVLKKTLA"
     gene            complement(4468..5868)
                     /locus_tag="VHM69_11075"
     CDS             complement(4468..5868)
                     /locus_tag="VHM69_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740979.1"
                     /translation="MSLDAANVNAQLSYTTSATANLKLSCQGIIDSHIAPTDSPWYGA
                     LNSELQQAKALAQEWQTKYDTTLKSDVLTRVVLCGQAFLAAKTNINQLFDQASSDPAG
                     AKAGLQAALGTLKAQVQIISTTVSSYETGLRGWGQRLNQVHDQMATTIGQIQAQENEL
                     QSQISSINSNMATLQAEIVNDRNAIADAESHRSAGIVETIFGIIFAPFTGGLSLILAG
                     IGVSSIAEAQSKVAAMESTINDYQARIISTQQNLTQDQAQLVTLNGLTFSAGIALSDI
                     DIASQMLDTVRTSWDAFFQEIGNVITKIANAQDAKSIIVEKAWFTAACNEWDLIVKGV
                     QGLIGVPMSMRNVKLDTSDVPVIRIVPTASHPPVPSDLKVTPMPGPNDLSVAPDCPVL
                     AWGNYTFWAVDHVDNRVAMSILGFDAYNRIVKQIEKQGARYLWQMTLDQSNETLTCYG
                     QANNKITVALSEIQMT"
     gene            complement(5965..7056)
                     /locus_tag="VHM69_11080"
     CDS             complement(5965..7056)
                     /locus_tag="VHM69_11080"
                     /inference="COORDINATES: protein motif:HMM:NF017595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HBL/NHE enterotoxin family protein"
                     /protein_id="HEX2740980.1"
                     /translation="MITAEAATSTNTNDKTASGFASLHIVTTSCHGVLNTQFRAPSPK
                     PKWFDDLSGKLDAAKVLANQWIDDIAPQMTASIPSHVIDYGTTYSAITDEIVRLLEAN
                     RNATRTSNPQVFQQVSELIKALHESVDSIITEIQDTQGQLKDWGDAMQTSHDDLFNGA
                     ANIQSAQTELGTEIDKMNNAIKGLRELIDGENKAIAVGAGAIGIGLFALVVGIAFAPV
                     TGGASLIVSGIGAAAIIGGAVDWGIMQSRINSQFSEIAKDQQKIDDDKRQLVALQGLS
                     LAANSAINSVATATQALSDVKVLWTTFQNELKGTMNNLDKADTALSAIVSEAQVKAAK
                     KEWDLAVDFAQQLSGVQLQVQAKELPMSA"
     gene            complement(7053..>7409)
                     /locus_tag="VHM69_11085"
     CDS             complement(7053..>7409)
                     /locus_tag="VHM69_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740981.1"
                     /translation="IVELRNKASQEAQAAAMSKGIIQLIDNFDKSLLAVQHQLPALGT
                     MWGNEKSKVDGAIAALHSGAQPTQYLELLSMEAATATWKKLADFAKQITQKAEQGKPV
                     ILMTSTQSSLKGASTP"
BASE COUNT         1510 a         2159 c         2307 g         1433 t
ORIGIN      
        1 aagtttgcgt aaaggttctg gcccctatag agttggggtt cggagaggcc catagaagga
       61 ggagttggag tgatcgctga cgttaccagg tcattaggga ccgactacta tctgctcgac
      121 gagttattca ctgacgagga gcgtgcgatc cgggacaggg tcagggcgtt cgccgacgcc
      181 gaggtcatcc ctatcataaa cgagtactgg gagaaggccg agttcccttt cgagctcgta
      241 cccaaggtcg ccgcgttgaa catcgccggc ggccccatcg aggagtacgg gtgtcctggc
      301 atgagccagg tcgcagccgg tctcgtcgcc atcgagatgg cgcgggggga cgggagcttc
      361 aacaccttct tcggggtaca ttccgggcta gcgatgggga cggttgccat ctgtggttcc
      421 gaggagcaga aacgaaggtg gctgcccggg atggcgagga tggagaagat cggggcctgg
      481 ggcctgacgg agccggatca cggctcggac tccgtgatgt tggagaccac ggcgaggcgc
      541 gacggggacg gctgggtact gaatgggaag aagcgttgga tcggcaacgc caccttcgcc
      601 gacctcgtca tcatctgggc gcgcgacgtg gaggacgggc aggtcaaagc cttcgtatta
      661 gagaaggacg agaacggaga gcatcacccc ggctactcca ctgagttaat aaaaggcaag
      721 atgggcaagc gcgccgtctg gcagcccgac atctttctcg atgacgtgcg cgtccccggc
      781 gagaacaagc tcgaaggcgc caactccttc aaagatacaa acaaagtatt gacggcaacg
      841 cgcagcgggg tcgcgtggga ggctatcggg catgccatag ccgcctatga agcggcgctg
      901 cagtatgcaa aagagagggt gcaattcggc aagcccatag cctcattcca gctcatccag
      961 aacaagctct ccaacatgct cgccgagatc accaacatgc agctcatgtg cttcaggctc
     1021 gcgcagctcc aggagcaggg caagatgacg gggccgatgg cctccctcgc caagatgaac
     1081 aacgccagga aggccaggca ggtatgcgcc gacgcgaggg acatcatggg cggaaacggc
     1141 gttcttctcg agtaccacgt cgcaaggcac ctctccgaca tggagatcgt ctacacctac
     1201 gagggaacgg acaccatcca gtcgctcatc gtaggacgcg acgtaaccgg cattagcgcg
     1261 ttcgtatagt tatcctgtga ggctcacctc acgcgtgggt ggcgacgggc agatgcgttc
     1321 gcggcgacgg agcgcagccg tatcgccatc acctctatcc aggtgagaat caccgcgagg
     1381 aagaggtaga acccccactg ccacacagaa acgaacaaca gagccaaata gaggatcgcg
     1441 gtgatcaggg tgtacaagtc gtagccacgc cagcgcggat ccctcctcag ccgccaccac
     1501 cagaagaaga gggagagcag caacgacagg acgagaagga taaaggctag caagtggatc
     1561 caaccgtgcc acgtctgggg cccctccgac aggtaagggt ccgtcttgaa acccgcgagc
     1621 accagcccga taccggcaac cacaaggagc gccgggctga tccatgaacc ggacgccacg
     1681 ccgcgatgga gcccgagtgc gaagacgatc agagtgaggc cgaaaaagac gaagttcaat
     1741 acctgtagcc aaccgaaggg tcccagggca agaccgctcg gccagggtac gtcggaggct
     1801 cctatgggat cccaccccag cctgaccatg aacccgtact gagcaacggt caagaccgcg
     1861 gcaacaaacg cgaacacggc tggtccgatc atgccggcga gggctgcatt cctgagcgac
     1921 actacttgat ctcctctctg ctttgcagga atgatgccac cggccgaaga caggcgcatc
     1981 tccaggtctc gcactcccaa cgagcagaag ctccggaggc gagtcacggt ctgctatctc
     2041 ttccgccggt aacggcccgc aaaaaccatt aagctgatac ctgacacctg aagcctgagc
     2101 gaccgaaggg agcgacatga aagccatccg actgcacgag atcgaaggcc cagagagcct
     2161 cgtatacgag aacatccccg agcctgagcc gggtgccggt gagatcgtgg tgcggctgca
     2221 cgccgccgcc ctcaaccgcc gcgacgtctt cgttacgcgt ggacagtatc ctggcgcgag
     2281 gccggatgcc ctgccggtga tcctcggctc cgacggctca ggcgaggtcg ccgcgcgcgg
     2341 cgacggcgcg gatggtccct ccgaaggtac ggaagtagtt atcaattcgg cgctgtactg
     2401 gggggacgac ccgcgtgtgc ccgggaagga gtacaggata ctcgggctcc ctgatgacgg
     2461 gacgttcgcc cagttcgtca aggtacctgc ggactacgtc ttccccaaac cctcgcacct
     2521 atcgcacgag gaggccgcgg cgataccgct cggcgggcag accgcctaca gggcgctggt
     2581 aacgcgggga cagatacaga atggcgagac cgtcgtcgtg ccgggcatcg ggagcggggt
     2641 tgcgaccttc gtcgtacaga tggcggctgc ccttggagcg cgggtgttcg tcacgagcgg
     2701 tagcgacgag aagatagaga aggcgaagga gctgggcgct gaaggggggg tcaattacaa
     2761 caacgaggac tggtcgaaag aactgaagaa aatggtcggc aaagtggacc tctccgtgga
     2821 ccacgtcggc ggcgaggcgt tcgacgcgct cgtctccctc gccgcgccgg gaggccgcat
     2881 cgtcaccttc gggtcgacgg ccgggccgaa agtatccgtc gtgatgccga ggatcttcct
     2941 caagcacctg accgtgctcg gcacggctat gggcacgccc gaggagttcg gtgcgatgct
     3001 cgacctctac gcggatcatg ggctcaagcc cgcgatcaac aagtccttcc cgctcgaaga
     3061 gaccgcctcg gcgatggagt acatggagaa gggcgtgggg atcgggaaga tcgtcctcga
     3121 cgtacccgcg tagcccgaga cgcgacttcg gaaagggtag acagccatcg gcgccgggag
     3181 cgagccgggc ttcgaccgcc tcgagacgga gcgcctggtc ctgcgccgtt tcgaagtcgc
     3241 cgatctctcg ccgttcctcg cgtaccgcaa cgacccgcgc gtagcaaggt accaggactg
     3301 ggattcatac gacgagcggg aagcgctcga ctttatccgg gccatgaaat cgtctgaacc
     3361 ggggtcgccg ggcgagtggt tccagttcgc cgtagtgcta aaagggaccg gagagccgat
     3421 cggggactgc ggtttgaaga ccgaagaaga cacgcggcag gcagagatcg gcgtaacatt
     3481 cgcccgagag caccagggca agggctacgc ctgcgaagcc gtgtcgcggc tccttgacta
     3541 cgctttcagt gacctgggaa tgcaccgcgt cgtcgccatc accgaccggc agaacgcgcc
     3601 ttcggtgacg ttgctcgagc ggctggggat gcgtcgcgag ggctcattca cccgaaacgt
     3661 gtggttcaaa ggtcgttggt cgagcgagta cctgtacgcc atcctcagag acgattggct
     3721 tcgaagacat gagcgggacg aaagggttgc gcagaagctc aggatcgtct cggaaccgca
     3781 tggctctccc ggagacgcca ccttcgtccg cgagggcctg gcgctcttca atgtagcgac
     3841 cacgggagac tcctactaca gcccgctcgc catctacctc aaggacgaga gaggggcggt
     3901 cctcggcggc gccctgggcc acgtctatgg aggatggctc gacctcgaat atctgtgggt
     3961 gatagagcct tttcgcgggc aggggcacgg agggaaactc ctcgaagcgg ccgaggacga
     4021 ggccagggca cagggatgcc acggcgtctt tttgacgacc ttcagcttcc aggcgcgacc
     4081 gttctaccaa aattttggct acgaggtcgt cgccgaaatc cccgaatacc cgagggggca
     4141 cagtcgctac gttctcaaaa agactctggc ctagcaggga tctcaattcc cgcgggacgg
     4201 atcggggccg tagtgttccc aagtagaaag ctggaccact ctccaaccat gcttgtctgg
     4261 cctttgcaga aggcctcctc tccatggcgt gatcggtcgt tcgtcggcgt aagaagactt
     4321 cgacgacggg tacgtctacc gcgtcgagga cggcacgtag agagacgttc agcaagaagc
     4381 tgagagtcgg gaatcgaggc tacaccggct tttccgttcg atggctcgaa ggcaagctac
     4441 gctctccagg aaacccgatc cactgcttca cgtcatctgg atctctgaca acgctaccgt
     4501 gatcttattg ttggcctgac catagcaggt cagcgtctcg ttcgactgat ccagagtcat
     4561 ctgccagagg tagcgggctc cctgtttctc gatctgcttg acgatcctgt tgtaggcgtc
     4621 gaagcccagg atgctcatcg caacgcggtt atccacatgg tccacggccc agaaggtgta
     4681 attcccccag gccagaaccg ggcagtccgg cgcgacgctc agatcgttcg ggcccggcat
     4741 gggagtaacc ttcaggtccg atggaacggg aggatggctc gcggtcggta cgatgcggat
     4801 tacaggcacg tcggaggtat cgagcttcac gttcctcatg ctcatcggga cgccgatcag
     4861 tccctggacg cccttcacta tgaggtccca ttcattacac gccgcggtga accaggcctt
     4921 ttcgacgatg atcgacttgg catcctgggc attggcgatc ttcgttatta cattgccgat
     4981 ctcctggaag aacgcatccc agctggttct aaccgtatca agcatctgcg aagctatatc
     5041 gatgtcgctg agcgcgattc ccgcggagaa cgtcaaccca ttcaaggtaa cgagttgtgc
     5101 ctgatcctgg gtcagattct gctgggtcga gatgatgcgt gcctggtaat cgttgatcgt
     5161 gctctccatt gcagcaacct tgctctgggc ctccgcaatg gaagagaccc cgatccctgc
     5221 aaggatcagc gaaagccccc cggtgaaagg agcaaatatg atgccgaaaa tggtctctac
     5281 gattcctgcg cttctgtgcg actcagcatc tgcgatggca tttcggtcgt tgacgatctc
     5341 ggcttgaagc gtagccatat tggagttgat ggaggagatc tgtgactgca gttcgttctc
     5401 ctgtgcctgg atctggccga tagtagtcgc catctggtca tgaacctggt tcaggcgctg
     5461 gccccagccc cgtagccccg tctcatagct tgaaacggtc gtggatatga tctggacttg
     5521 cgccttgagc gtgcccagcg cggcctgtaa tccggccttc gccccggccg gatcactcga
     5581 agcctggtcg aagagctggt taatgttcgt cttggcggcc aggaatgcct gtccgcatag
     5641 caccacgcgg gtcaggacat ccgatttcag cgtggtgtcg tatttcgtct gccactcctg
     5701 cgcgagcgcc tttgcctgct gcaactcgga attcagggca ccgtaccagg gcgaatcggt
     5761 cggcgcgata tgggagtcga taattccctg gcaagagagt ttcaggttcg ccgtggcgct
     5821 cgtggtgtaa gaaagttggg cattgacgtt ggcggcatcc agactcatcg tgaaggctcc
     5881 attgcttctt tcgattccca gggctcaatg atgcgaacgg ggcgcgagcc ggcccgcgcc
     5941 ccgtccttcg agagtagagc gagatcaggc actcataggc agttccttgg cctggacctg
     6001 caactgcacg ccagacagtt gctgggcgaa atccacggcc aggtcccatt ccttcttggc
     6061 ggccttgacc tgggcctcac tgacgatggc ggacagggct gtatccgctt tgtcgaggtt
     6121 gttcatggta cccttcagct cgttctggaa cgtcgtccag agcaccttga cgtcggagag
     6181 ggcctgggtc gcggttgcaa cggagttgat cgccgaattg gcggccagcg acagaccttg
     6241 cagcgccacc agttgacgct tgtcgtcgtc gatcttttgc tggtccttgg cgatctcgct
     6301 gaactggctg ttgatcctgg actgcataat gccccagtcc acggcgccgc cgatgatggc
     6361 cgctgcaccg atcccggaca cgatcagtga agcaccgccg gtaactggcg caaaggcaat
     6421 gccaactaca agagcaaaca ggccgatgcc aattgcgccg gcccctacgg cgatcgcttt
     6481 gttctcgccg tcgatgagct cgcggagtcc cttgatagcg ttgttcatct tgtcgatctc
     6541 ggtacccagt tccgtctgcg cggactgaat gttggcggca ccgttgaaca ggtcgtcgtg
     6601 ggacgtctgc atagcgtcgc cccagtcttt caactggccc tgagtgtctt ggatctccgt
     6661 gatgatcgag tcaacgctct cgtgcagagc cttgatcagc tcggaaacct gttgaaagac
     6721 ctgcggattg ctcgtcctcg tggcattccg attggcttcg agcaaccgca cgatctcgtc
     6781 tgttatcgca gaataggtcg taccatagtc gataacgtgg ctcgggatgc tcgccgtcat
     6841 ctgtggcgcg atgtcgtcga tccactggtt cgccaggacc ttggcggcgt ccagtttccc
     6901 gctcagatcg tcaaaccatt taggcttcgg cgacggtgcc ctgaactggg tgtttagaac
     6961 accgtggcag gaagtggtca cgatgtgcaa agacgcaaac ccgctggccg tcttgtcgtt
     7021 tgtgttggta ctagttgctg cttccgcagt gatcatggcg tcgatgctcc tttcaggctc
     7081 gactgtgtac tggtcatgag gattacgggt ttgccctgct cggccttctg ggtaatttgc
     7141 ttcgcgaagt ccgccaactt cttccaggtt gcggtcgccg cctccatgga gagcagctcg
     7201 agatactggg tcggttgggc gccggagtgg agcgcggcaa tagccccatc gaccttcgac
     7261 ttttcgttcc cccacatcgt gccgagagca ggaagctgat gttgaacggc gagcaagctc
     7321 ttgtcgaagt tatctatgag ttgaatgatc cccttgctca tcgccgcggc ctgtgcttct
     7381 tgtgaagctt tgttccgaag ctcgacgat
//
LOCUS       DATAIR010000456         3787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444016, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000456 DATAIR010000000
VERSION     DATAIR010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444016"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /gene="cobA"
                     /locus_tag="VHM69_11090"
     CDS             complement(<1..602)
                     /gene="cobA"
                     /locus_tag="VHM69_11090"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX2740982.1"
                     /translation="MIYLVGSGPGDPGLFTVKGVRCMEEADAVVYDRLAPEALLAYAK
                     PEAERIYVGKKPGDPTMSQEDINALLVELGRTGKTVVRLKGGDPYIFGRGGEEALSLI
                     EAGLPFEVVPGVTSGVAAPAYAGIPVTHRNVSTSVAFVTGHEDPTKGRSDVDWKKLAN
                     GADTLVLYMGVGRLKEISTGLIAAGKSPDTPVACIRWGTRP"
     gene            complement(599..1507)
                     /gene="hemC"
                     /locus_tag="VHM69_11095"
     CDS             complement(599..1507)
                     /gene="hemC"
                     /locus_tag="VHM69_11095"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2740983.1"
                     /translation="MILGTRGSKLALVQAEACAASLRATGIQVEIHVIRTTSEYHPDT
                     PLAIIDQRDVFTRQLDEAVLSGEIDLAIHSMKDVPTETPDGVSLAAVTRRHDPSDVLV
                     SDERYKVEELPHGAVVATSSLRRRAQLLHYRPDLEIVGIRGNVDTRVRKMREGAADAV
                     VLARAGLERLGLDAPYSVIPPDIVLPAVGQGALAASTLANHPLRESIRETLNHGPTER
                     VVLAERAMLRALEGGCRVPVGVSSAVAGNVVRLRGVVISPDGAFVYRGEAEGAEPEEV
                     GQRLARELLEQGAAAVLREIREVGSR"
     gene            complement(1504..2799)
                     /gene="hemA"
                     /locus_tag="VHM69_11100"
     CDS             complement(1504..2799)
                     /gene="hemA"
                     /locus_tag="VHM69_11100"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX2740984.1"
                     /translation="MLVAVAGMSHRSAPVEVRERVAFPPCAGRNFIRRLKDDGVAAEA
                     VLLSTCNRTEVYAVVEDEGARERLLDVLAEDRGVGRASLERDMYWLTDAEAVRHLYRV
                     ASSLDSMVVGEGQILGQVREAYRAATEEHCSGQVLNRLFHTSLRVGKKVRSETGIGEN
                     SLSVPRVAVKLAEEVFGSLTGRRALVLGAGDMSELVVKHLKDRGVADLLIANRTPERA
                     SLLAARVGGRAVAFDAIAAELPEVDVVVSSTGSGEWVVESGAVAGALELRSEPLFFID
                     IAVPRDVDPVVQTLPSVYLYDIDDLQAVVERNAEGRQDAAEQGEAMISPAVLEFTGWL
                     STLHVVPLIQELRDGAEQIRRHELARALKRMDLSPEDEADVERMSHSLVNKLLHGPIQ
                     EIKARAEAGSPLESSEVRRRLLALDGLDVELHRPRHESP"
     gene            complement(2775..3335)
                     /locus_tag="VHM69_11105"
     CDS             complement(2775..3335)
                     /locus_tag="VHM69_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX2740985.1"
                     /translation="MEGENTVLYPIFLELSGRRCVVVGGGVVATRKARKLLQAGAEVV
                     VISPEVHPELANMDVEIQRRPYEYGDLADAYIAFTATDSREVNAAVALEARERGVPIN
                     VADLPAEGDFAVPSTLRRGRLQVAVSTGGASPTLARRIRSELEAVFGPEWAGVVEEFG
                     VTRRNGEEPGEAFEEEVSRCLSRLRG"
     gene            complement(3456..>3787)
                     /gene="moaC"
                     /locus_tag="VHM69_11110"
     CDS             complement(3456..>3787)
                     /gene="moaC"
                     /locus_tag="VHM69_11110"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX2740986.1"
                     /translation="LRETALPKGDVLNTARIAGVMAAKKTPELIPLCHGLNLTSVEVE
                     FEVGHNRVGITATARASDRTGVEMEALIAVSVAALTVYDMCKAVDKGMVIGEIRLLEK
                     TKEPVGP"
BASE COUNT          642 a         1333 c         1142 g          670 t
ORIGIN      
        1 gggcgggtac cccaccttat gcaggcgacg ggcgtgtcag ggctctttcc tgcagcgatg
       61 agaccggtgg agatctcctt caaccggccg acacccatgt acagaacgag cgtgtccgca
      121 ccattggcca acttcttcca gtccacgtcc gagcgaccct tcgtggggtc ctcgtgtccc
      181 gtgacgaacg cgacgctggt cgagacgttc ctgtgcgtga cgggtatacc agcgtatgcc
      241 ggtgccgcga cgccgctcgt cacgccgggg acgacttcga aagggaggcc agcctcgatc
      301 agcgagagcg cctcctcgcc gccgcggccg aagatgtaag ggtcgccgcc cttaaggcgc
      361 acgacggtct ttccggtacg tcccagttcg acgaggagcg cgttaatgtc ttcctgagac
      421 atcgtggggt cgccgggctt cttgcccacg tagatccttt ctgcttcggg cttcgcgtac
      481 gcgagaagcg cttcaggggc aagcctgtcg tagacgacgg catcggcctc ctccatgcac
      541 ctgacgccct tcacagtaaa cagcccggga tcacctggac cgctgccgac gaggtagatc
      601 acctgctccc cacctcccgg atttcacgga gcaccgccgc tgccccctgc tccagcaact
      661 cacgcgccag cctctgacct acctcctcag gctcggcgcc ctccgcctct ccacggtaaa
      721 cgaatgctcc gtcaggggag atcacgacac ctctgaggcg caccacgttt cccgccacgg
      781 cgctgctcac cccgacaggg acccggcagc cgccttcgag cgcgcgaagc atcgcccgct
      841 cggccagcac aaccctctcg gtcggcccgt ggttcagagt ttccctgatg ctctcacgca
      901 ggggatggtt ggccagcgtc gaggcggcca gagcaccctg gccgacggcg ggaagcacta
      961 tgtcaggtgg aatcacggag tacggggcgt cgaggccgag tcgttcgagg ccggcgcggg
     1021 cgagcaccac ggcatcagca gcgccctcgc gcatcttgcg aacgcgcgta tccacgttgc
     1081 ccctgatacc gacgatctcc aggtccggcc tgtagtgcaa gagctgcgcc cgccgcctaa
     1141 ggctggatgt ggcgacgaca gccccgtgcg gaagttcttc caccttgtag cgttcatccg
     1201 agacgagcac gtccgacggg tcgtgacgtc gggtgacggc cgcgagcgag accccatccg
     1261 gagtctccgt cggcacgtcc ttcatggagt ggatcgcgag gtctatctcc ccagagagca
     1321 cggcttcgtc gagttgtcga gtgaagacgt ccctctggtc gattatagcg agcggggtgt
     1381 cggggtgata ctcgctggtc gtcctgatga cgtgaatctc gacctggata ccggtggcgc
     1441 gaaggcttgc ggcgcaggct tcggcctgca cgagggcgag cttcgagccg cgggtaccga
     1501 ggatcacggg gattcgtggc gtgggcggtg cagctccacg tcgagcccgt caagggccag
     1561 caagcgccgc cttacttcgg agctctcgag tggggatccc gcctcggcgc gggcttttat
     1621 ctcttggatc ggaccgtgca gcagcttgtt gacgagcgag tggctcatcc gctcgacgtc
     1681 ggcctcgtcc tcgggcgaga gatccatcct cttgagggcg cgggcgagct cgtgacgccg
     1741 tatctgctcg gcgccgtcgc gaagttcctg tattagcggg acgacgtgca gcgtcgagag
     1801 ccagcccgtg aactcgagca cggcggggga gatcatggct tccccctgct ccgcggcgtc
     1861 ctgtcgccct tccgcgttgc gctcgacgac ggcctggagg tcatctatgt catagagata
     1921 gacgctcgga agcgtctgaa ccacagggtc cacgtccctc ggcaccgcga tgtctatgaa
     1981 gaacagcggt tcactcctca gctccagcgc ccccgcgacc gccccgctct cgacgaccca
     2041 ctcaccggat cccgtggagc tcacgacgac gtccacttcc ggcagttcag cggcaatggc
     2101 atcgaacgcg acggcccgcc ccccaaccct cgcggcgagt aggctggcgc gctcgggggt
     2161 acggttggcg atgaggaggt cggcgacgcc cctatccttt aggtgcttga ccacgagctc
     2221 gctcatgtcg ccggcgccga ggacgagtgc ccgccttcct gtgagcgaac cgaagacctc
     2281 ctcggcgagc ttcacggcta cgcgtggcac ggagagtgag ttctctccta tccctgtctc
     2341 ggagcggacc ttcttgccga ccctaagcga cgtgtggaag aggcggttga ggacctggcc
     2401 ggagcaatgc tcctctgtcg ccgccctgta cgcctcacgg acctggccca ggatctgacc
     2461 ctccccgact accatggagt ctagggaaga cgcaaccctg tacaggtgcc gcacagcctc
     2521 cgcgtcggta agccagtaca tgtctctttc gagggacgcc cgcccgacgc ccctatcctc
     2581 cgcgagcaca tcgagcagcc gctccctcgc cccctcgtcc tcgaccacgg cgtagacctc
     2641 ggtgcggttg caggtcgaga gcaacacggc ttcagcggcg acgccatcat ccttcaggcg
     2701 tctgatgaag ttccggcccg cgcaaggcgg gaacgcgacg cgctcgcgca cctcgaccgg
     2761 cgccgagcgg tggctcatcc ccgcaaccgc gacaagcacc ggctcacctc ctcttcgaag
     2821 gcctcgcccg gctcctcgcc gttccgacgc gtaacaccga actcctcaac gacgccggcc
     2881 cactctggcc cgaagaccgc ttccaactcg ctcctgatgc gcctcgccag cgtgggcgaa
     2941 gcgccgcccg tcgagacagc cacctggagc cgtcccctcc gcaacgtcga gggcacggcg
     3001 aagtccccct ccgccggaag gtcggcgacg ttgatgggca cgccgcgttc ccttgcttcc
     3061 aacgccacag cagcgttgac ctcccgcgaa tcagtcgccg taaaagcaat gtacgcatcc
     3121 gccaggtcgc cgtactcgta ggggcggcgc tgtatctcca cgtccatgtt cgcgagctcg
     3181 gggtggacct cgggggagat caccacgact tcagcacccg cttgcaacaa cttgcgggcc
     3241 ttcctggtcg cgaccactcc gcccccgacg accacgcatc gcctgcccga gagttccagg
     3301 aatatggggt aaaggacagt attttcgccc tcgatctctg ctcctgacct agcgaatagc
     3361 ccacgggtta gccaggaagt ctagcataac cgtcacgtct atatcattgt ctaacaaagt
     3421 gttgcatgca ccgcagcgaa gcgccgtatg aaccttcacg gcccgacggg ttccttggtc
     3481 ttctccagga gcctgatctc cccgatcacc atccccttgt ccacggcctt gcacatatca
     3541 tagacggtca acgcggccac gctcaccgcg atgagagcct ccatctcgac gcctgtgcgg
     3601 tcgctcgcgc gagccgtcgc cgtgatgccg acgcggttat ggccaacctc gaactccacc
     3661 tcaaccgagg tcaggttcag gccgtggcag agcgggatca gctcgggcgt cttcttggcc
     3721 gccatcacac cagcgatcct ggccgtgttc aagacatcgc ccttgggcaa cgccgtctcc
     3781 cgcaaca
//
LOCUS       DATAIR010000457         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_197746, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000457 DATAIR010000000
VERSION     DATAIR010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197746"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VHM69_11115"
     CDS             <1..1009
                     /locus_tag="VHM69_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HEX2740987.1"
                     /translation="FPTYFKWKNPQPNLIVNCTESEPGYWADKVLHRDYFDEFLDLFD
                     VMKEIFGIENIIMGVHFKDEEWFSRYDESADGLYQVVHVPDKYSMGEEKTLIKNIFRE
                     NKEKWVPRRVEMPDGSKRPGLPLDAGHIVNNSETLLNIYNAFFLGKPVTTKYLQVFGP
                     DLELKLFEAPLGSSATELVKLAGVDVEAEAGKLSVIDGGPYLNEMGIESLGEGDAYVR
                     RTTNGFLVIPKGVASKEYAEIKTKRPDDVISLVGKVSGVSIPLGGRFLKPATPLVSEG
                     DEVSFEQKIGEPVDEGFSIGVWSGMDGTISSIEEDVVQISGGGVPQEEAAAETEAEAA
                     R"
     gene            1191..2270
                     /locus_tag="VHM69_11120"
     CDS             1191..2270
                     /locus_tag="VHM69_11120"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX2740988.1"
                     /translation="MERSSSNNSPLVDSHLDLAENATLFGRDLTMSVAETRASERRTT
                     RQATVSLPELERGGIAVAFATVTAGFLAEDVGENFEPGSAIYNTPEEAEAQALAQIRL
                     YEGWEREGRARLLKSVNDLDHHLRLWKDDRKPGLVMLMEGADPIVDVRDLPRWWRRGL
                     RMIGLTFGDTNYGTGVGGGSPTFKQGGLTPEGFELLGQMADLGIVWDISHLAEEGIWQ
                     GLELGLPRVCASHANARALIPTDRHLSDEVIRAVAERNGVMGLVLYNGFLEPRWRRDN
                     SLSVTLDEHLRRHASHIAALVGWEHLGIGSDLDGGFGLEESPLEIDTVADLYKVGSVV
                     PAEVREAVLGANWLDFLRSSLPQSS"
     gene            complement(2394..2741)
                     /locus_tag="VHM69_11125"
     CDS             complement(2394..2741)
                     /locus_tag="VHM69_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740989.1"
                     /translation="MNIFFDIQGTLVSGGRPRPHTRDVFLRLLEMGHHVYLWSSGGSA
                     YAASAARLLGVEDLVFGCFGKAAPPPVTVDFVVDDQPGMAEHYGGYGISAFAGDPDDR
                     VLLSVPGAVESRR"
     gene            complement(2870..>3182)
                     /locus_tag="VHM69_11130"
     CDS             complement(2870..>3182)
                     /locus_tag="VHM69_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2740990.1"
                     /translation="DSVEFRVPQVPIVSAMDGAVLTSADAVKEALKGQMVAPVRWVKV
                     VETLAKMRVEEWIELGGGGVLTRMLKDFELPSLSGITFEDLRARLYGQAQNLLPRKED
                     E"
BASE COUNT          670 a          921 c          992 g          599 t
ORIGIN      
        1 tttccccacc tacttcaagt ggaagaaccc gcagccgaac ctgatcgtga actgcaccga
       61 gtctgagccg ggctactggg ctgacaaggt gctccaccgc gattacttcg acgagttcct
      121 cgatctcttc gacgtgatga aggagatctt cgggatcgag aacatcatca tgggcgtcca
      181 cttcaaggac gaggagtggt tctcgcgtta cgacgagagc gccgatggtc tatatcaggt
      241 agtccacgtc cccgacaaat actcgatggg cgaggagaag acgctcatca agaacatctt
      301 cagggagaac aaggagaagt gggtcccgcg ccgggtcgag atgcctgacg gttcgaagcg
      361 cccgggcctc ccgctcgacg ccgggcatat cgtcaacaac tccgagacgc tcctcaacat
      421 ctacaacgcg ttcttcctcg gcaagcccgt cacgaccaag tacctgcagg tcttcggacc
      481 cgacctcgag ctcaagctct tcgaggctcc gctcggatct tcagcaacgg agctggtgaa
      541 gctggcgggc gtggacgtcg aggccgaggc aggcaagctc tctgtcatcg acggcggacc
      601 ctatttgaac gagatgggga tagagtcgct cggtgagggt gacgcctacg tcaggcgcac
      661 caccaacggc ttcctcgtca ttcccaaagg cgtggcttcc aaggagtacg cggagatcaa
      721 gaccaagcgt cccgatgatg tcatctcgct tgtcggcaag gtcagcggcg tgagcatccc
      781 gctcggaggc aggttcctca agcctgcgac cccgctagtc tccgagggcg acgaggtctc
      841 cttcgagcag aagatcggcg agcctgtcga cgagggcttc tccatcggag tgtggtcagg
      901 tatggacggc accatctcct caatagagga ggatgtcgtc cagatctccg gcggcggagt
      961 tccgcaggag gaggcggcgg ccgagacgga ggcagaagca gccaggtagg cttcaggtta
     1021 caggtttcag agggccgggg tgcaccccgg cctttccttt tggaggcccg caaatgggct
     1081 gtgtactctt cctggcaatg tccagagcaa tagaacattg ttagtgcaca aaatactctc
     1141 tggccagggg ccggatccaa accccgttcg caagcatcga cggagcatga atggagcgat
     1201 catcgtcgaa taattcgccc ctcgtggatt cgcatctcga tctggcggag aacgcgacgc
     1261 tgttcgggcg tgacctgacc atgagcgtcg ccgaaacaag agcatccgaa agacgcacga
     1321 cgaggcaggc taccgtctcc ctccccgaac tagagcgggg tggcatagcc gtggcgtttg
     1381 cgaccgtgac cgcgggtttc ctggcagagg acgtcggtga gaactttgaa cccggatcgg
     1441 ctatctacaa tacacccgag gaagccgaag cacaggccct tgcccagatc cggctctacg
     1501 aaggctggga aagggaggga cgcgcgaggc ttctcaagtc tgttaacgac cttgaccatc
     1561 atctgcgatt gtggaaagac gatcgcaagc ctggcctggt gatgcttatg gaaggcgccg
     1621 acccgatcgt tgacgtacgc gaccttccca ggtggtggcg gcgcggtcta cgcatgatcg
     1681 ggcttacgtt cggagacacg aactacggaa ccggggtagg cggaggcagt ccgacgttca
     1741 agcagggagg cttgacgccg gaaggtttcg agttgctcgg gcaaatggca gacctgggca
     1801 tcgtctggga catttcgcat cttgccgagg agggtatatg gcaagggctg gagctagggc
     1861 tcccccgcgt ctgcgcgtcg cacgcgaatg ctcgggctct gatcccgaca gacaggcatc
     1921 tgagcgacga ggtcattcgc gctgtagccg aaaggaatgg ggtaatgggt ctcgtcctct
     1981 acaatggttt tctcgagcct cgctggagac gagataattc gctatccgtc acgctcgacg
     2041 aacacctgcg acgacacgcg agccacatcg cggctctcgt cggttgggag catctgggaa
     2101 taggttcaga tctggacggg ggcttcggtc tcgaagaaag cccattggaa atagataccg
     2161 tggccgacct gtacaaagtg gggtccgtcg tacccgccga ggttcgagag gcggtactgg
     2221 gcgcgaactg gctggatttc ctcaggtctt cattgcccca aagttcgtaa cggatcctta
     2281 gcatctaatc gcgacggata caagaattct cgtagcccct gaagcaggaa agcgtcgcat
     2341 agcacggagg tcaggaagag gaggagcacg acctcaataa gaatttgccg gctctagcgc
     2401 ctcgactcta ccgctccggg cacgctcaaa aggacccggt cgtcggggtc tcccgcgaag
     2461 gcggatatac cgtatccgcc gtagtgctcc gccatgccgg gctgatcgtc gacgacgaaa
     2521 tccacggtta ctggtggtgg agcagccttg ccgaagcaac cgaatacgag gtcttccaca
     2581 ccgaggagcc tcgccgcgct cgccgcgtag gcgctacctc cgctcgacca gaggtagacg
     2641 tgatgcccca tctccaagag ccgcaggaaa acgtcgcggg tgtggggacg agggcgtcca
     2701 ccggagacca gggtaccctg tatgtcgaag aagatgttca tttgtaggtt tcaggcatca
     2761 ggcgcctagc gcatcacttg tgtcggaaca cgtgtcagcc tcgctgatca ggcagcacga
     2821 gagggcatcg ctgtacaagg cctttcctga agcctgatgc ctgaatgacc tattcatctt
     2881 ccttgcgtgg caggaggttc tgggcctgcc cgtagaggcg ggcgcgcagg tcttcgaagg
     2941 tgatgccact aagagaggga agctcgaagt ccttgagcat cctggtaaga acgccgccgc
     3001 cgccgagttc gatccactct tccacccgca tcttcgccag ggtttcgacg accttgaccc
     3061 agcgcacagg ggcgaccatc tgtcccttga gcgcctcctt gacggcgtcg gcgctcgtga
     3121 gcaccgcacc gtccatggcg ctgactatgg ggacctgcgg tacgcggaac tcgacggaat
     3181 cg
//
LOCUS       DATAIR010000458         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_151978, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000458 DATAIR010000000
VERSION     DATAIR010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151978"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(847..1245)
                     /locus_tag="VHM69_11135"
     CDS             complement(847..1245)
                     /locus_tag="VHM69_11135"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2740991.1"
                     /translation="MLGIGLSAVMHLVRPWGLAGNKRLYGGAGWTLVGAGVAISASAV
                     RTASDVDLERPPTLISGGPYAISRNPMYLGWTSLYLGGALITRNAWMVASLPLLAGLI
                     HREVLREEHALEGAFGEEYTRYRKLVRRYL"
     gene            complement(1510..3534)
                     /locus_tag="VHM69_11140"
     CDS             complement(1510..3534)
                     /locus_tag="VHM69_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2740992.1"
                     /translation="MGIAGDIALILVAALLGGIVAQRLGLPLILGYIAAGVLVGPNTL
                     GPSVGNVHQIELLAEIGVALLLFTIGLHFSLKELAPVRRIALFGTPVQMALTIAFGYG
                     LGVLLLDLGWVESVWLGALISLSSTAVVLKTLGEQGVLGTLSARVMIGILIVQDLAIV
                     PLLTVLPVLEDVGEGIPELGLALLKAAAFIAAMLFLGSRVLPLILARLATWGSRELFL
                     VSVVAIGLGVGYATYLFGLSFAFGAFVAGMVLSESDYSHQALAEVEPLRDVFAMIFFV
                     SIGLLIDPMFLWESAGIIALVVALVIVVKGMIFGGLARAFGYGNIAPIAVGMGLFQVG
                     EFSFLIAREGISTDAISQGTYSLVLATAAITMALTPLAARLTPVLYGRYRRRFPREPL
                     RTFNLPEGGLRGHAVVAGYGRVGSFVARLLSRLDKPFVVVEANPGRAEESREADFPTV
                     NGDASAIPVLDAAGVGEARLVIVTIPDAIAARLAVLRVKSLAPNADVLVRAESVEQLE
                     DLGRLGVYEAVQPELEAGLELARQALARFGVAAEEAQSFADGVRKELYASISAEDSAS
                     EDDEFLARLRQASRVIEVEWVRLPEISSRDNGDSDKDVFFGRTIGDLRVRSETGASVL
                     AVVRGEKVIPNPGADLRLEPGDAVGVLGTPEQRIAFRELMREPMQDDAVT"
     gene            3661..5115
                     /locus_tag="VHM69_11145"
     CDS             3661..5115
                     /locus_tag="VHM69_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX2740993.1"
                     /translation="MSVLNVRVIVNALAAAVAAVGAVMLVPGGYSLLTGSDEGWIFLL
                     PGGVTLASGMSLFYFTRARQSAYISRQSIFLTVVVCWLGVGLVGAVPFLFSGLMGPVD
                     AWFNSVAGFTTTGAATVAPEDLSRALLLWRSVSQWIGGLGIIVLFVAIAPQAGFGATQ
                     LYTAEAATPVQERITPRIKDTVKVLAFVYGGLTLGGVVVLHLAGMGTFDAVNYALSTV
                     STGGYSTHSNSVADFDSWVVELAIAAGMVLAGTNFAIYYTAARRGLGRAVGNAELITY
                     LGVILASTMLITIDLIASGHRYSSAVAFREAFFQSASLTTGTAFSTTNWSAWPPFSVT
                     LLVLVMAVGGCAGSTAGGMKAIRVYLLTRHAAQDIFRLVHPRAIMPLVLGDRVLPERI
                     RVGVLGFFFVYVATMAVGTLLIAMHQVPIGHAFGSVFSCVNITGAFPGAAGTSQFYAD
                     LPSTAKTTLAILMLLGRLELFTVLVLLSPAFWRA"
     gene            5410..>5863
                     /locus_tag="VHM69_11150"
     CDS             5410..>5863
                     /locus_tag="VHM69_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX2740994.1"
                     /translation="MKGKITRRRFLGAATAGAAWVALGGTVGCEPETRAKATARAKAP
                     TAQGDQTWAFHSRPDLKPPVIRVTTQAQDTAPGYVFCAPKSGPGESGPGQDGCLILDN
                     TGQPVWFRPAPDEAMDAMDFKVQTYKGETVLTWWEGHHTSYGQGEFVIC"
BASE COUNT         1125 a         1814 c         1839 g         1085 t
ORIGIN      
        1 aaacgcccag ggtgacgtgg tatggagcct gtcgaccgag acgctcttcg cgctcaccgg
       61 tctgaatagc ctgacgttca ggtgggacct ggacgaagag gtgtgggacc gttacaagtc
      121 gcagctgggc atcacgggag ggccgaggtt gcgggtattg gtctggcgct ggtaagggtg
      181 ggtgcgtccc gatagagcac agaatggcgg aggcagcagg gaggcaagcc gtcgagacac
      241 gggcgtgcca tgaaaagaag cgccataaga tcggagcagg cgtctttctc gtagctcaat
      301 ttgcttagta aaaggtggct cacatggggg acgcggaacc gactgcgcgt agctatcatc
      361 aggctatgaa agagcctggt aaagagagtg aatcaaacct gcacctcccg cccggatacc
      421 acgcgcaact tgaccccgag gtgttgatct tgagacgtgc cgacggcttt catgtcgcgc
      481 tattcagccg cggagggttc gtggctgagc gcgtggagca agctgcctgg gcagactacg
      541 ataggcccga ggggccatcc tggttctgta cttctagcga gcgtccttct cgtcgtcacg
      601 gtcttccctg gtcacctcgc tggcccggat gaggtggggc acccaagctg cgccaccttg
      661 agacgcagtc tcccagtcca acggtcgtca aagcgcaata gtgacagaag aggtgagtgg
      721 gcgaatccgc cggcgcgctt ttttcctaca agataaggta ttcacaattg ccctgttcga
      781 acgagcctgc ggaaggtgcg tcctccgcac gccgggtgtc tgatcgctgg tcttgaacac
      841 cagccactag aggtagcgac gaaccagctt ccggtacctg gtgtactctt ccccgaacgc
      901 tccctccagc gcatgctctt ctcgcagaac ctccctgtgg atgagccctg ccaataatgg
      961 taacgaggcg accatccacg cgtttcgtgt gataagcgca cctccaagat agagtgaggt
     1021 ccatcccaaa tacatcgggt tgcggcttat agcgtagggt ccgccggata tgagcgtggg
     1081 ggggcgttcc aggtcgacat cggaggcggt tcgtaccgcc gatgctgaga tcgcgacccc
     1141 agccccgaca agggtccatc cagcgccgcc atacagtcgc ttgttcccag caagtcccca
     1201 tgggcgaacg aggtgcatca ccgcgctgag tccaatcccg agcatgtgag gctcgggaag
     1261 tgggatgttg ttccatctcc acgaatatga catttcttct gtcagttcgc ctccttccga
     1321 agttcgttat gctcatcatc gggaccagaa gagttctact agaggctagc agacgcgccc
     1381 ggattcagca gagtgcgaca tatgatccca ttcagactcg ctgggtccgc caagtctcac
     1441 gcgtcggaga tggtgttctc tatcgaactc ctaccgcaaa gccatcctgc gtggcggata
     1501 agcctcaggt caggtgacag catcgtcctg catcggctcc cgcatcagct ctcggaaggc
     1561 gattcgctgc tcaggtgtgc cgaggacgcc caccgcgtcg ccgggctcga gtcgtagatc
     1621 cgccccaggg ttcgggatca ccttttcgcc ccgcacgacc gccagaacgg acgcccctgt
     1681 ctcgctcctc acccgcaggt ctccgatagt gcgtccgaag aagacgtctt tgtcggaatc
     1741 accattgtcg cggctactga tctctggcaa gcgaacccac tctacctcga tcacccgaga
     1801 ggcttgccgt agccgcgcga ggaactcgtc gtcctcggac gcactatcct ccgcggagat
     1861 ggaagcgtac agctccttgc gtaccccatc agcgaagctc tgagcctcct cagccgcgac
     1921 accgaagcgc gcgagggcct ggcgggccaa ctcaaggccg gcctcgagct caggctgcac
     1981 cgcctcgtag acgccgaggc ggccaagatc ttctagctgc tcgacgctct cagcccggac
     2041 caggacgtca gcgttcgggg cgagcgactt tacccgaagg acggcgagcc tggccgctat
     2101 ggcgtcgggg atggttacga tgaccaggcg cgcttcgcca accccggcgg catcgaggac
     2161 gggtatggcc gaggcgtcac cgttgaccgt ggggaagtcc gcctctctag actcctcggc
     2221 gcgcccgggg ttagcctcaa cgaccacgaa cggtttatcg agcctgctga gaagccgggc
     2281 gacgaaggag cccacccgac cgtatccggc gacgaccgca tggccgcgca gtcctccttc
     2341 gggcaggttg aaggttcgca ggggttctct tgggaacctc ctccggtacc gtccgtaaag
     2401 gacgggcgtc agacgggctg ccaggggggt aagggccatc gtgatcgccg cggtggcgag
     2461 cacgagcgag tatgtaccct gcgagatcgc gtccgtgctg atcccctctc gggcgatcag
     2521 aaacgaaaac tctccgacct ggaagagccc catccccacc gcgatgggcg cgatgttacc
     2581 gtagccgaat gctctggcga ggccaccgaa gatcatgccc tttacgacga tgacgagcgc
     2641 cacgaccagc gcgatgatcc ctgcactctc ccacaagaac atcggatcga tcagcagccc
     2701 gatcgaaacg aagaagatca tggcgaagac gtccctcaag ggctcgacct cggcgagggc
     2761 ctggtggctg tagtcggact cggagaggac catcccggcg acgaacgccc cgaaggcgaa
     2821 ggatagcccg aagaggtagg tcgcgtagcc gaccccgagg cctattgcca ccactgagac
     2881 gagaaacaac tcgcgcgagc cccaggtcgc cagacgcgcg aggatgaggg gcagtaccct
     2941 ggaccccagg aagagcatcg cggcgatgaa cgccgccgcc ttgagcaggg cgagcccgag
     3001 ttcggggatg ccctcgccca cgtcctctaa gaccggaagc acggtcagca gcggtactat
     3061 cgcgaggtcc tggacgatga gtatgcctat catgaccctg gccgacaggg tgccaagtac
     3121 cccctgctcg ccaagggtct ttaggaccac agcggtgctc gagagggaga tcagggctcc
     3181 cagccacacc gattcaaccc accccaaatc caggagtaat acccccagcc cgtatccgaa
     3241 ggcgatcgtg agcgccatct gcaccggtgt gccaaacaag gcgatgcgcc gcaccggggc
     3301 gagctccttg agtgagaagt gcagcccgat cgtgaaaagc agtagcgcca ctccgatctc
     3361 cgcgagtagc tctatctggt gcacgttccc taccgaggga cccaatgtat tcggcccgac
     3421 gaggacgccg gcggcgatgt aaccgaggat caagggtagc ccgagccttt gggccacgat
     3481 ccccccaagg agggcggcca cgaggatgag tgcgatgtcg ccggctattc ccattgttgt
     3541 gcacacctta ctacgtagcg atgcctcctg agaggttctg gaattggggc gccagcgagt
     3601 agcccgatgg tagagtgagc gaggcatgag gatagtcgca accgtaggca tctaatcaat
     3661 atgagcgttc tgaacgtgag ggtgatcgtt aatgcgctgg cggcggccgt tgccgccgtg
     3721 ggcgccgtga tgctcgttcc agggggctac tcgctgctta cgggttcgga cgaaggttgg
     3781 atcttcttgt tgcccggggg tgtcactctg gcctcgggga tgtctctttt ctacttcacg
     3841 cgggccaggc aaagcgccta catatcgaga cagagcatct tcctcacagt ggtggtttgc
     3901 tggctgggtg tggggctggt cggtgctgtg ccgttcctct tctccgggct gatgggtccc
     3961 gtggatgctt ggttcaactc cgtagccggc ttcaccacca ccggagccgc aacggtcgcg
     4021 ccggaagacc tttcacgcgc cctgttgctc tggcgcagcg tgagccagtg gatcgggggc
     4081 ctgggcatta tcgtgctgtt cgtggccatc gcgccgcagg ccgggttcgg cgccacccaa
     4141 ctctacacgg ccgaggcggc gacccctgtg caggagcgga taacaccgcg catcaaggac
     4201 acggtcaagg tgcttgcctt cgtgtatggc ggccttactc tggggggcgt cgtagtactg
     4261 catctggccg ggatgggaac attcgacgcc gtcaactacg cgctctccac ggtctcgacg
     4321 ggcgggtact ctactcattc gaactcagtg gccgacttcg actcctgggt ggtcgagctg
     4381 gcgatcgcgg ccgggatggt gctggcaggc acgaacttcg ccatctacta cacagcggcc
     4441 cggcgtggat tgggacgcgc cgtcggcaac gctgagctca tcacctactt aggcgtcatc
     4501 ctcgcgagca cgatgttgat aactatcgat ctaatcgcct ctggccaccg gtactcgtcg
     4561 gccgtcgcgt tccgcgaagc cttcttccag agtgcgagcc ttacgacggg gacggccttc
     4621 agcacgacga actggagcgc ctggcctccg ttctccgtaa cgctgctggt gctggtcatg
     4681 gccgtcggcg gatgcgccgg ctcgacggca ggggggatga aggctatccg ggtctacctg
     4741 ctgacgcgcc acgccgcaca ggacatcttt cgcctggtcc acccgcgggc aatcatgccc
     4801 ctcgtgttgg gcgacagggt gctgcccgaa cggattcggg tcggggtcct cggtttcttc
     4861 ttcgtgtacg tagcaaccat ggccgtcggc accctcctca tcgctatgca ccaggttccc
     4921 atcggacatg cttttggctc ggtgttctcg tgcgtgaaca ttacgggcgc gttccctggc
     4981 gcagccggga cctcgcagtt ctacgccgac ctgccctcaa cggccaagac cactctcgcc
     5041 atcttgatgc tcctgggccg cctggagctc ttcacggtgc tggttctgct cagcccggcg
     5101 ttctggcgtg cctagaaggt ctgtctcggc aagcgcggtc acaatgaact tattggagct
     5161 aaccgagaaa cggcccgaaa aatctggaga gactgtccga aaaggtctcc tgagagcggc
     5221 tccatagtgc ggtggcgtct ttaccgcagc gtccacatca tttccacttc gactacgtcg
     5281 gcagtggatg caccggacga aggaggtgca atagtggcca taaatagtgt accttttggg
     5341 cgcccgaaaa tgctggcagg cggcaagcgt tgccgtcctg cgcaacgtac aaggaagtga
     5401 cgcaacgact tgaaagggaa gatcacacgc cgaaggttcc tcggggcggc caccgctgga
     5461 gccgcgtggg tcgcgctggg cggaaccgtg ggctgcgagc ccgaaacgcg cgccaaggct
     5521 actgccaggg cgaaagcccc gaccgcgcag ggagatcaaa cgtgggcctt ccactcgcga
     5581 ccggacctca agccgcccgt catcagggtg accacgcaag cacaggacac ggctcctggc
     5641 tacgtcttct gcgccccgaa aagcggccca ggtgagtccg ggccagggca ggacggttgc
     5701 cttatcctcg acaatacggg ccaacccgtg tggtttcgtc ccgctccgga cgaggcgatg
     5761 gacgcgatgg acttcaaagt gcagacatac aagggcgaga ccgtactcac ctggtgggag
     5821 ggccaccaca ccagctacgg ccagggtgag ttcgtgatct gcg
//
LOCUS       DATAIR010000459         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_121925, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000459 DATAIR010000000
VERSION     DATAIR010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121925"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1268)
                     /locus_tag="VHM69_11155"
     CDS             complement(210..1268)
                     /locus_tag="VHM69_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2740995.1"
                     /translation="MPKKLVAVAPRKAALVDYDEATVGRNQVKVRVEYAAPKHGSEVS
                     IFRGEDPFVADLFDEDWRLFLERNPEVREAENGDGPVLGNQWVGVIVEKGGEVERLGV
                     GDRVCGYGGIQETQIVDSVDNPYLLKVPDTMPWKSALCFDPAQFALGGIRDGHVRLGD
                     RVAVFGLGAIGTIAAQMAKVAGASYVAVVDPIEKRRHPAIEAGADDAFDPLNQDVGLE
                     LKKATGRAGVDVAIETSGNEQALQQALRGLAYGGTIAFVGWARAFSGTLDLGREAHFN
                     NASLVFSRASSEPSRDHPRWDRRRIAMSCWDLLANGAIDCERIVDPVVTFDEAPRAYE
                     EYVDRNPQLSVKLGVTFI"
     gene            complement(1300..>2216)
                     /locus_tag="VHM69_11160"
     CDS             complement(1300..>2216)
                     /locus_tag="VHM69_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2740996.1"
                     /translation="RRGPVPGPPAPLHKRADGWWSQRMTAFRYSYNVIGFSGEEVAES
                     IDRLARLGYDAVEVEGEPERYDPKRIRKFAEDAGLSVGSVCPNFTAERDLSHPDPVVR
                     KGALDYLRAVSEFAAEVGAPLFIVAPTAYARVQPMADPRDEWRWAVEGIRTAGEHAAS
                     LEVDLTLECWNRYGTYMLNRLDDGARMWRETGLSNGGIMADTFHMNIEESSITGAIRS
                     FADILNHVHLSDSNRLAPGLGHIDFEEVLRTLREVGYRGGLAFELIPALPDILEDEPE
                     RPSFDEVASQAIGHMREAERSLAARQET"
BASE COUNT          390 a          750 c          655 g          421 t
ORIGIN      
        1 caacaggtgg gtacgtagca ccggatcggc ggccttgcgg tagagcacct ctaaacggcg
       61 ggactcggat ccctcggaac tgcgtctctc atccatgccc cgaagtatac agccggattc
      121 cgtattactc gcatgagagt aggctcaata tacactccga gagatgcggc atcttagcag
      181 caaagtaagg atgtctgctg gaaagaccct cagataaaag ttacaccgag ctttacgctg
      241 agttgaggat tgcgatccac gtactcctcg taagcgcggg gagcctcatc gaaggtaacg
      301 acggggtcga cgatgcgttc gcagtcgatc gcgccgttcg cgagcaggtc ccagcagctc
      361 atcgcgatac ggcgacggtc ccagcgggga tgatccctgc tcggctcgct gctcgccctg
      421 gagaatacga ggctggcgtt gttgaagtgg gcctccctgc cgaggtcgag ggtgccgctg
      481 aaagcacgcg cccacccgac gaaggcgatg gtgccaccgt aggcgaggcc cctgagcgcc
      541 tgctgcaagg cctgctcgtt gccgctggtc tcgatcgcca cgtcgacgcc cgccctgcct
      601 gtcgccttct tcagctccag cccgacgtcc tggttcagcg ggtcgaaggc gtcgtcggcc
      661 cccgcttcga tggccggatg cctgcgcttc tctatggggt cgacgacggc cacgtacgag
      721 gcccctgcca ccttcgccat ctgggcggct atcgtcccga tggcgccaag cccgaagacc
      781 gcgacgcggt ctcctagcct cacgtgcccg tcgcggatgc cgccgagggc gaactgcgcg
      841 ggatcgaagc acagcgcact cttccacggc atggtgtccg gtactttcaa gaggtacgga
      901 ttgtccactg agtccacgat ctgggtctcc tgtatgcccc cgtagccaca cacgcggtcc
      961 ccgacaccca acctctcgac ctcgcccccc ttctccacga tgacgccgac ccactggttg
     1021 ccgagcaccg gaccgtctcc gttctctgcc tcacggacct cagggttacg ctccaggaac
     1081 aaacgccagt cctcgtcgaa gaggtcggcg acgaaggggt cttccccacg gaagatactc
     1141 acctccgagc cgtgcttcgg ggccgcgtat tcgaccctga ccttcacctg gttccggccc
     1201 acggtcgctt cgtcgtagtc aacgagagca gccttcctcg gcgcaacggc gactagcttc
     1261 ttcggcattc ccgtcctctt ctgcttgtca caggacgttt cacgtctcct ggcgggctgc
     1321 caggctccgc tccgcttctc tcatgtgtcc tatggcctgg ctggcgacct cgtcgaatga
     1381 gggccgctcc ggctcgtcct ccagtatatc ggggagagca gggatcaact cgaacgctag
     1441 cccgccccgg tacccgactt cccgcagcgt tcgcaggacc tcttcgaaat ctatgtgacc
     1501 caggccgggg gcgagacggt tggagtcgga gaggtgcacg tggttcagga tgtccgcgaa
     1561 cgagcgtatc gctcccgtta tggagctctc ctcgatgttc atgtggaacg tgtcggccat
     1621 gatgccgccg tttgaaagcc ctgtctcgcg ccacatcctg gcgccatcgt ccaacctgtt
     1681 cagcatgtag gtgccgtagc ggttccagca ctccagtgtg agatccacct ccagagacgc
     1741 agcgtgctcg cccgcggtcc ttatcccttc aacggcccac cgccactcgt cgcgggggtc
     1801 ggccataggt tggacgcgcg cataagccgt cggggcgacg atgaagagcg gcgcgccaac
     1861 ctcggcggcg aactctgaaa ccgcacgtag atagtccagt gcacccttcc gaactacggg
     1921 gtcagggtga gagaggtcgc gttccgcggt gaagttcggg cacaccgagc cgaccgaaag
     1981 tcccgcgtcc tccgcgaact tcctaattct cttcgggtcg taccgctctg gttccccttc
     2041 gacctcgacg gcgtcatagc ccaggcgcgc cagccggtcg atcgactccg cgacttcctc
     2101 gcccgaaaag ccgatcacgt tgtacgaata gcggaacgca gtcatccttt gagaccacca
     2161 gccgtcagcc cgtttatgaa gcggcgctgg aggcccagga acaggaccac gacggg
//
LOCUS       DATAIR010000460         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_306122, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000460 DATAIR010000000
VERSION     DATAIR010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306122"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHM69_11165"
     CDS             <1..984
                     /locus_tag="VHM69_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX2740997.1"
                     /translation="LRILQVAEARGEGVAEAREFLREVYPKLLAWHRYLLTQRDPEGS
                     GLVTIYHPWESGTDNSPRWSAALERGEVGEIPEYERLDLEHVPDPSERPTGLEYDRFI
                     WLVEVIKEARCHDGVLYDTCPFLVKDVLASAILVRANEALLEIAGIADASDEDREKIR
                     TWIERGRQGLEGRWDPEIGLCLDYDLRTGEPLRARTIAGFAALIAGIGKLDALLRVLD
                     SHAFTGNPDLRRPLPPSASPDGPGFQPRNYWRGPVWPVMNWLLWWSLKKAGERKRAAR
                     LKSDALAQLSDGCFGEYYEPFTGEALGSNEQSWTAAVALDWLAEEEIPESP"
     gene            complement(1007..1774)
                     /locus_tag="VHM69_11170"
     CDS             complement(1007..1774)
                     /locus_tag="VHM69_11170"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX2740998.1"
                     /translation="MKRATVLDELAEAAVVRAEELRASTDLDILYQEALLFENRDFAG
                     ALAAPGLSVISEIKRASPSAGFIREADPAEWGARYESEGASCLSVLTEPDRFEGSLED
                     LDAARNNTTLPVLRKDFTVDEAQILETGKRADAILLIAALFDAAALARYVSLAVEIDL
                     TPLVEIHDEQEANLALESGARVLGVNNRDLRDFTVDLGTFERLAPKLAGATLVAESGV
                     KSPEDARRLRDAGADAVLVGEAAMRDPGLVARISNIP"
BASE COUNT          315 a          605 c          660 g          329 t
ORIGIN      
        1 ctgcgcatcc tccaggtcgc ggaggcaagg ggcgagggag tcgccgaggc cagggagttc
       61 ctgcgcgagg tatacccgaa gctcctggcg tggcaccgct acctgctcac gcagcgggac
      121 cccgaaggct cggggctcgt aaccatctac catccgtggg agagcggcac ggacaactct
      181 ccgcgctgga gcgcggcgct cgagcggggc gaggtcgggg agatcccgga gtacgagcgg
      241 ctggacctgg agcacgttcc tgacccgtca gagaggccga cgggcctcga atacgaccgg
      301 tttatctggc tcgtcgaggt tatcaaggag gcccgttgcc acgacggtgt tctctacgac
      361 acttgcccct ttctggtcaa ggacgtactg gcgagcgcga tcctggtgcg ggccaacgaa
      421 gcgttgctgg agatagccgg aatcgcagac gcctcagatg aagaccggga gaagatccgg
      481 acctggatcg aacgcggccg ccagggtctg gagggacgct gggaccccga gatcggcctc
      541 tgcctggatt acgacctgcg cacgggtgaa ccgctcaggg cacgcaccat cgcgggcttc
      601 gcggcgctga tcgccggcat aggaaagctt gatgccctcc ttcgcgtcct cgactcgcac
      661 gcgttcacgg gcaaccccga cctccgcagg ccactgccgc cgagtgccag cccggacgga
      721 cccggcttcc agcctcgcaa ctactggcgc ggtccggtgt ggcccgtgat gaactggctc
      781 ctgtggtggt cactgaagaa agcgggcgaa cgaaagcggg cagccaggct caagagcgac
      841 gccctcgcgc agctttcgga cgggtgcttc ggcgagtact acgagccgtt caccggcgag
      901 gccctgggat ccaacgagca gtcatggacc gccgccgttg ccctcgactg gctcgctgaa
      961 gaggagattc ctgagagtcc gtgaggatag gtgacgtctt atcgactcag ggaatgttcg
     1021 agatcctcgc gacgagacca gggtcgcgca tggccgcttc gcctaccagc accgcgtctg
     1081 cgcccgcgtc gcgcagacgc cgggcgtctt cggggctctt gacgccgctc tcggcaacga
     1141 gggtcgcgcc ggcgagcttc ggggcgaggc gttcgaaggt gccgaggtcc acggtgaagt
     1201 ccctgaggtc gcggttgttg acgccgagga ccctggcgcc tgactccagg gcgaggttcg
     1261 cctcttgttc gtcgtggatc tccactagcg gcgtcaggtc gatctccacc gccagagaga
     1321 cgtagcgggc gagcgccgcc gcgtcgaaaa gcgccgctat gagcaagatg gcgtctgccc
     1381 gctttccggt ttcgaggatc tgcgcctcgt caacggtgaa gtccttgcgg aggacgggaa
     1441 gtgtggtgtt gttccgggcg gcatcgaggt cttcgaggct cccctcgaag cgatcgggct
     1501 cggtgaggac ggagaggcag ctcgcgcctt ctgactcgta gcgggcaccc cactccgcgg
     1561 ggtcggcctc gcggataaat ccggctgagg gcgatgcgcg ctttatctct gagatcacgg
     1621 aaaggccagg tgccgctaat gcgccggcga agtccctgtt ctcgaagagc aacgcttcct
     1681 ggtagaggat gtcgaggtcc gtgctggcgc gcagttcctc cgccctgaca acggcggctt
     1741 cggcgagctc gtcgagtacg gtggcgcgct tcatgcggtc cccgagaggc ggcgggtagt
     1801 tttgacgaag tcttccaggg ccctcgctgc cgtcccgccg ctgatggact cccgagccag
     1861 acgcacgcct tcttctatcg tctcagctct gcccgagacg tggatcgcg
//
LOCUS       DATAIR010000461         7619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_122054, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000461 DATAIR010000000
VERSION     DATAIR010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122054"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1244)
                     /locus_tag="VHM69_11175"
     CDS             complement(3..1244)
                     /locus_tag="VHM69_11175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX2740999.1"
                     /translation="MAEGRPEAYITGIGVVTPIGVGRKKFWSSLLANESGAGPITLFD
                     SDGFDVQIAAECTDFDPATFMDRKVAARTDRFAQMGLASAKLALEDAGAWDVLNQDPE
                     RVGLILGSGIGGVMSMETTQATIDSKGPSRVNPFSVTKIMPNSAAAHVGIQLGVQGPS
                     ITGALACTCGTDMMGLGYDLIRRGDADIVLCGATEAPLSPVIVAGFIAMRAMSRSNDD
                     PEGACRPYDVDHSGFLIGEASAVMVLESAESVERRGSEPYAKITGVGRTTDAYNLTDP
                     DPKGLGIMRAMQFAVADAGLESGQIGYINPHGTGTVAGDGPESQAMARINPNVQVSAT
                     KSTLGHSMGATGAVETAICALAVKERTIPPMRNLTKVAEGCADLDYVVDEPREAPDLE
                     AAICANLGVGGHNAAVVLERA"
     gene            1411..1713
                     /locus_tag="VHM69_11180"
     CDS             1411..1713
                     /locus_tag="VHM69_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741000.1"
                     /translation="MIFYAATVLFLANFALGVLVQLRIVDTKPFRWLHHALFFAVFAS
                     AAVATGVGFLEGEPYRWALLPVLVLFAVLPRVRAGTRGHVALASGALILYATGWML"
     gene            1724..2548
                     /locus_tag="VHM69_11185"
     CDS             1724..2548
                     /locus_tag="VHM69_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2741001.1"
                     /translation="MDFLEVLKRRRTTNGPFLPDPVKPEHQRLLVEVAAMAPSHFNSQ
                     PWRFVLVDDRETIDEVARISGESMTRLLEEGTFWKRYRPYFRFTEAEMEEKRDGIHFD
                     QMPGVLKPFSRQIFSNTGQAVMNRFGVPKTLGEDNRKLVAGSPLLLAVLLDKTEYRPG
                     ELSGFYSIFGMGAAVENVWLTTTELGMGIQFVSTPMEIPENWERMKRLFKVPDDLELM
                     AVYRLGYVPKERRRPTIDWSSRQRKRLSQYVFRNSCDVPESDSEPDLATPKRPEQR"
     gene            2545..3591
                     /locus_tag="VHM69_11190"
     CDS             2545..3591
                     /locus_tag="VHM69_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX2741002.1"
                     /translation="MSDPRILSVASALPPHRVGQEEAKAFARGMFSRTHGDFERLLPI
                     FDNVHVENRYFCVPVEWFEQDHTFPEKNALYVEHALDLSEKAARRALDRLGAEPDEVG
                     AIFFVSTTGLSTPSLDSKLIFRLGLSEHTRRVPIWGLGCSAGAAGIARAAEHARLYPD
                     QLVLLVAVELSGLTFQRGDRSKSNLISTSLFADGAAVVLIGGTNGPEIIGGYSTTWPD
                     TEDVMGWDLVESGLKVQLSKSVPTIVREKFRDNLEDACDSLGLDFADIRHFVLHPGGS
                     KVLDAFEEVLGLQPGGLATSRDVLRDCGNMSSVTVLFILERFLEGVEAASGDLGVLSA
                     MGPGFSAEHVFFRC"
     gene            3572..4183
                     /locus_tag="VHM69_11195"
     CDS             3572..4183
                     /locus_tag="VHM69_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEX2741003.1"
                     /translation="MCSSDADGSLQASEVSGFRFQASGLAGGWEVALNGVLLVAGVAL
                     VGLQRLLELRYSRRNELRLRAKGAVERGRGHYPVMVTIHTLWLASTLVEGLQRGPEIP
                     SWWPVPFAAFLLVQPLRYWAILSLGENWNVRILVVPGGKLVRSGPYRYFPHPNYVVVA
                     VEVLTFPLIFGAWITAVVFSILNAAILYVRIRTENRALKELAG"
     gene            4245..5519
                     /gene="serS"
                     /locus_tag="VHM69_11200"
     CDS             4245..5519
                     /gene="serS"
                     /locus_tag="VHM69_11200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2741004.1"
                     /translation="MLDLKYIRENAKAVEENSKNRGVEVDVVLVVELADRRSALIQDL
                     NELKQRQNQMAKSISRERDEEARGRLIEESRALKEQLPSKERDLHEVEERLREEQLKI
                     PNITHPDSPIGKDDTENVEIRRWGEVPDFSFEPKDHVELGEHLGIIDFDAGAKSTGSK
                     FYFLRGDAVLLELGLIRYAMDIMMERGYQPTITPDLARDQMLVGTGFIPRGPETQIYS
                     VEDSDLSLIATAEITLAGQHAEEILDESRLPLRYAGLSHCFRTEAGSHGRASRGLYRV
                     HQFTKVEMFAFTTPDQSEDIHNEMVEIEENIFQGLGLPYRVVDICTGDLGGAAYRKYD
                     LEAWMPGRGDFGEVTSTSNTTDYQARRLGICYRREGGRPQLLHTLNGTALAMSRALIS
                     LLEIYQQQDGSISLPEALVPYVGKTTIEPTAL"
     gene            5549..6157
                     /locus_tag="VHM69_11205"
     CDS             5549..6157
                     /locus_tag="VHM69_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741005.1"
                     /translation="MMSRSERVYWLLLLAYPREFRRDCGVEMAQVFGDLCREEKRRSG
                     AFGLMRAWVRTVPDLVSTAFAERVRGKDEEVYMQDRRLAVIGFVLLLAPLYFVSASLL
                     KYGLGVGFLFDPLEAFLSVAGRREIFNLISPVVFLGGLGLALALNTYAVTRLDVSRED
                     GTVVSTVRVKLELWNIAVAVVSILLLVTLIGYVFLENFTYRH"
     gene            6216..>7619
                     /gene="proS"
                     /locus_tag="VHM69_11210"
     CDS             6216..>7619
                     /gene="proS"
                     /locus_tag="VHM69_11210"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2741006.1"
                     /translation="MAEAVEKLVKKSEDPSRWYLQLVRMAKLADYGPVRGTFAIRPYG
                     YEIWERVQRDLDDRFKATGHKNAYFPLLIPESYLKKEAEHVEGFDPELAWVTIGGREE
                     LEERLAIRPTSESIICDFYRNWIQSYRDLPILINQWCNVLRWEKVTRPFLRTAEFLWQ
                     EGHTVHATADEAREETLRMLGVYSDCFHEAMAIPVLTGAKSPSERFAGAVETFTCEAL
                     MGDGRALQAATSHDLGQNFARAFDITFLDENQDRVHPYQTSWGFSTRTIGGLILVHGD
                     DRGLKLPPGIAPTQAVVVPIWRGKNKGEVRREAEALYTELKDSGLRMEVDLDEEHSPG
                     WKFNEHELRGVPVRVELGPRDIENGQAVLVRRDTGEKEFVERKELPHRLNTLMVEIQE
                     NMLRNAETFREENTRRAESYEEFREIIARKRGFVVAPWDGTEETEQKIKDDTKATIRL
                     LPFEREEGEDLVSGKPGK"
BASE COUNT         1402 a         2345 c         2451 g         1421 t
ORIGIN      
        1 catcaggccc tctccaggac cacggccgcg ttgtgtcctc cgacgccgag gttggcgcag
       61 atggctgcct cgaggtcggg cgcctcgcgc ggctcgtcga cgacgtagtc gaggtcggcg
      121 cagccttccg ctactttcgt gaggttcctc atcgggggga tggtcctctc cttgacggcg
      181 agggcgcaga tcgcggtctc caccgcgcct gtcgcgccca tggagtggcc cagggtggat
      241 ttcgtcgccg agacttggac gttggggttg atccgggcca tcgcctgcga ctcggggccg
      301 tcgccagcca ccgtgcctgt gccgtgtggg tttatgtagc cgatctgccc gctttcgagc
      361 cccgcgtcgg cgacggcgaa ctgcatggcc cgcatgatgc cgaggccctt cgggtcgggg
      421 tctgtgaggt tgtaggcgtc ggtagtccgc ccgacgcctg tgatctttgc gtacggctca
      481 gacccgcggc gttccacgga ctcggcgctc tcgaggacca tcaccgccga agcctcaccg
      541 atgaggaacc ccgagtggtc aacgtcgtag ggtcggcagg cgccttccgg gtcgtcgttg
      601 gaccggctca tcgcgcgcat cgcgataaag ccagcgacga ttaccgggga aagtggcgct
      661 tcggtggcgc cgcagagcac tatgtcggcg tcgccgcgcc ggatcaggtc gtatccaagg
      721 cccatcatgt ccgtgccgca ggtgcaggcg agcgcgcccg tgatcgaggg cccctgcacg
      781 ccgagttgta tgccgacatg ggcggccgcg gagttgggca tgatcttggt gactgagaag
      841 gggtttacgc gggaagggcc cttgctgtct atggtcgcct gggtcgtctc catgctcatg
      901 acgcccccga tgccgctgcc gaggatgagc ccgacccgct cggggtcctg gttgagcacg
      961 tcccacgccc ccgcgtcttc gagggcgagt ttggccgagg cgaggcccat ctgtgcgaag
     1021 cggtcggtac gcgccgcgac cttcctgtcc atgaatgtcg cggggtcgaa gtccgtgcac
     1081 tcggcggcga tctggacgtc gaagccatcg gagtcgaaga gggttatggg accggccccg
     1141 ctctcgttgg ccaggagcga gctccagaac ttcttgcgcc cgacaccgat cggggtgacc
     1201 accccgatcc ccgttatgta agcctccggg cgcccttccg ccatctcgcc ccctagcaaa
     1261 agcttcgttc tacaaggtcc atcgaactac gaatcttagc accacaggcc cttctcgact
     1321 cctgcgccgc tctacatgcg gcgagagggc atacaattgg cgggcgatgc ctgtgctata
     1381 accctccagg atgagtccga ggagcgagca ttgatcttct acgccgcaac ggttctgttt
     1441 ctcgcgaact tcgcgcttgg tgtgctggtg cagcttcgta tcgtggacac gaagcctttt
     1501 cgctggctcc atcatgccct tttcttcgcg gtcttcgcct ctgcggccgt cgccacgggc
     1561 gtcggcttcc tcgagggcga gccgtaccgg tgggcgctgc tccctgtcct ggtcctcttc
     1621 gcggttctgc cgcgggtgcg ggctggcacg cgcgggcacg tcgcgctcgc gagcggggca
     1681 ctgatcctct acgctaccgg ctggatgctt taggaggctg agattggatt tcctggaggt
     1741 cctcaagaga cgcaggacga cgaacgggcc cttcttgcca gaccccgtga aaccggagca
     1801 ccagcgtctg ctcgtcgaag tcgcggcgat ggccccttcc cacttcaaca gccagccctg
     1861 gcgcttcgtg ctcgttgacg accgcgagac catcgatgag gtagccagga taagcggcga
     1921 gagcatgacg cgtctgctcg aagaaggaac tttctggaag cgctaccgtc cctacttccg
     1981 attcaccgag gccgagatgg aggagaagcg agacggaatc cacttcgacc agatgccagg
     2041 cgtcctcaag cctttttcgc gccagatctt ctcgaacacg ggccaggccg tgatgaaccg
     2101 cttcggcgtc ccgaagactt tgggcgagga taaccgcaag ctcgtcgcag gctcgcccct
     2161 cctactggcg gtgctgcttg acaagaccga gtaccgtcct ggcgagctct cgggcttcta
     2221 ctccatcttc gggatggggg ccgctgtcga gaacgtctgg ctcaccacga cggagttggg
     2281 aatgggcata caattcgtct cgacgccgat ggagatccct gagaactggg agcggatgaa
     2341 gaggctcttc aaagtccccg acgacctcga gctaatggca gtctaccggc tcgggtatgt
     2401 cccgaaggag cggcggcggc ccacgatcga ctggtctagc cgtcagcgca agcgcctctc
     2461 gcagtacgtc ttccgtaatt catgcgatgt tcccgaatca gactcggaac cagatctcgc
     2521 caccccgaag aggccggagc aacgttgagc gaccccagga ttctctccgt ggcgagtgcc
     2581 ctgccgcccc accgcgtcgg gcaggaggag gcgaaggcct tcgccagggg aatgttctcg
     2641 cggacgcacg gcgacttcga gcgtctgctc ccgatcttcg acaacgtaca tgtcgagaac
     2701 cgctacttct gcgtgcccgt cgagtggttc gagcaggacc acaccttccc cgagaagaac
     2761 gccctctacg tcgagcacgc gctcgacctc tccgagaagg ccgccaggag ggcgctggac
     2821 aggctcgggg ccgagccgga cgaagtaggt gcgatcttct tcgtctcgac gaccgggctc
     2881 tcgacgccgt cgctggactc gaagttgatc ttccgcctcg gcctctccga gcacacgcgg
     2941 cgagtcccga tctgggggct cggttgctcc gccggcgcgg cgggtatcgc ccgcgccgcg
     3001 gaacacgccc gcctgtatcc ggaccaactc gtactcctcg tcgccgtcga actctcgggc
     3061 ctcaccttcc agcgcggcga ccgctcgaag agcaacctga tctccacgag tctcttcgcc
     3121 gacggcgcgg cggtcgtgct gatcggaggc acaaacggcc cggagattat cggcggttac
     3181 agcacaacgt ggcccgatac cgaggatgtc atgggatggg atctcgtgga gtcgggcctg
     3241 aaggtgcaac tctccaagag cgtcccgact atcgtacggg agaagttccg cgataacctg
     3301 gaggacgcct gcgactctct cggcctcgac ttcgcagaca tcaggcattt cgtgctgcat
     3361 cccggcggat cgaaggtgct cgacgccttc gaagaggtgc tcggcctgca acccggcggc
     3421 ctcgccacga gccgcgacgt cctgcgcgac tgcggcaaca tgtccagcgt cacggtactt
     3481 ttcattctgg aacgctttct cgaaggcgtc gaggcagcat cgggcgacct cggtgtcctc
     3541 tcggcgatgg gaccaggttt ctcagcggag catgtgttct tcagatgctg acggctcgct
     3601 gcaagcatct gaggtttcag gcttcaggtt tcaggcttca ggtcttgccg gtgggtggga
     3661 ggttgctctg aacggcgtct tgctcgtcgc cggtgtggcg ctggtcgggc tccagcgttt
     3721 gctggagctt cggtactcgc ggcggaacga gcttcgcctg cgggcgaagg gggcggtgga
     3781 gcgggggagg ggccactatc cggttatggt caccatccat acactctggc ttgcctcgac
     3841 gctcgtagag ggccttcaac gcgggccgga gattccttcg tggtggcctg tgccgttcgc
     3901 cgcgttcttg ctcgtccaac cgctacggta ctgggcaata ctctcgcttg gggagaactg
     3961 gaacgtgcgg atactcgtcg tgccaggcgg aaagctcgtc cggagcgggc cgtaccgcta
     4021 cttcccgcat cccaactacg tcgtcgttgc cgtcgaggta ctgaccttcc ccctcatctt
     4081 cggtgcctgg atcacagcgg tcgttttctc gatactcaac gccgcaattc tctacgtacg
     4141 catccgaaca gagaaccggg cgttgaagga gcttgcaggc taatataacg tcggctgata
     4201 gcttcttgct gatacctgaa acctgccgac caaagggagg cactttgctc gatcttaaat
     4261 acatccgtga gaacgcgaaa gccgtcgagg agaactccaa gaaccgcggc gtcgaggttg
     4321 acgtggtcct ggtagtggaa ctggccgacc gtcgctcggc gctcatccag gacctgaacg
     4381 agctcaagca gcgccagaat cagatggcga agagcatcag tagggagcgt gacgaggagg
     4441 ctcgggggag gctaatagag gaatcgcggg ctctgaagga acaactcccc tccaaggaaa
     4501 gggatcttca cgaggtcgag gaacggttgc gggaggagca gttgaagatc ccgaacatca
     4561 cccaccccga ctcgcctatc ggcaaagacg acaccgagaa cgtcgagatc cgtcgctggg
     4621 gcgaagtgcc ggacttttcc ttcgagccga aggaccacgt cgagctcggt gaacaccttg
     4681 gcatcataga cttcgacgcg ggggcgaagt caacggggag caagttctac ttcctgcgcg
     4741 gcgacgcggt gctgttggag ctcggcctca tccgctacgc catggacatc atgatggagc
     4801 gcggctacca gccgaccata acgcccgacc tggcgcgcga ccagatgctc gtcgggacag
     4861 ggttcatccc gcgcggacca gagacccaga tctactccgt cgaagactct gacctctccc
     4921 tgatcgcgac cgcggagatc acacttgcag gccagcacgc ggaagagatc ctggacgaaa
     4981 gtcgactccc tttgcgctac gcaggcctct cccattgctt ccgcaccgag gctggctcgc
     5041 acggccgcgc gagccgcggc ctctaccgcg tccaccagtt caccaaggtc gagatgttcg
     5101 cgttcaccac gccggatcag tccgaggaca tacacaacga aatggtcgag atagaggaga
     5161 acatcttcca gggtctcggc ctcccgtacc gcgtggttga catctgcacg ggagacctcg
     5221 gcggcgcggc ataccgcaag tacgacctgg aagcctggat gccaggcaga ggcgacttcg
     5281 gggaagttac gagcacctcg aacaccaccg actaccaggc ccgccggctc ggtatctgtt
     5341 accggagaga aggcggacga ccccaactcc tccataccct caacggcacg gccctcgcca
     5401 tgagccgagc cctgatctca ctcctcgaga tctaccagca acaagacggc tccatctcac
     5461 tccccgaagc cctcgtaccc tacgtcggca agacgacgat agagccaacg gctttgtagc
     5521 cgatcctcca ggcaaggata ttgagaccat gatgtcgcgt tccgagcgtg tctactggtt
     5581 gctgctcctg gcgtacccga gggagttcag gagggattgc ggggtagaga tggcgcaggt
     5641 gttcggggac ctttgccggg aggagaagcg gcggagtggg gcattcggtc tgatgagggc
     5701 ctgggttcgg acggtgccgg atctggtgtc taccgcgttc gcggagaggg tcaggggcaa
     5761 agatgaggaa gtatatatgc aggatcgcag gctggccgtt atcggcttcg tactccttct
     5821 cgccccgctg tacttcgtgt cggcgtcgct tctgaagtac gggctcgggg tagggttctt
     5881 gttcgatcca ctcgaggcct ttctgtccgt cgcaggacga cgagagatct tcaacctgat
     5941 ctcaccggtc gtgttccttg gtggattggg tctcgcgctt gcgctgaaca cctacgccgt
     6001 tacgcggctc gacgtaagca gggaagacgg gacggtcgtg agtacggtgc gggtcaagtt
     6061 ggaattgtgg aacatagcgg tcgccgtcgt gagcatactg ttgctggtaa cgttaatagg
     6121 gtacgtcttc ctcgagaact tcacttatcg ccactaggct gtcggacgct atactctctt
     6181 gctgattcaa cttccatcag gagaggagcg cccggatggc cgaagcggtc gagaagcttg
     6241 tcaagaagag cgaggacccg agcaggtggt atctgcagct cgtgcggatg gcgaagcttg
     6301 cggattacgg gccggttcgg gggacgttcg cgatcaggcc ctacggctac gagatctggg
     6361 agcgcgtcca gcgcgacctg gacgaccgct tcaaggcgac gggtcataag aacgcctact
     6421 tccctctgct catccccgag agctacctca agaaggaggc tgagcacgtc gagggcttcg
     6481 accccgagct cgcgtgggtc acgatagggg gcagagagga gctcgaggag cgcctcgcga
     6541 tccgtccgac gtcagagagc atcatctgcg acttctacag gaactggatc cagagctaca
     6601 gggacctccc tatcctgata aaccagtggt gcaacgtcct gcgctgggag aaggtaaccc
     6661 gtccgttcct cagaacagca gagttcctct ggcaggaggg gcataccgtc cacgcgacgg
     6721 cggacgaggc gcgcgaggag accctgcgca tgctcggcgt ctacagtgac tgcttccatg
     6781 aggctatggc cattcccgtc ctcaccggcg ccaagagccc ttccgaacgg ttcgccgggg
     6841 ccgtcgagac ctttacgtgc gaggcgctca tgggcgacgg acgcgcactg caggcggcga
     6901 ccagccacga cctcgggcag aacttcgccc gagctttcga catcacattc ctggacgaga
     6961 accaggacag ggtgcatcct taccagactt cctgggggtt ttcgacgcgg accatagggg
     7021 gtttgatcct ggttcacggc gacgacaggg gcctcaagtt accacccggg atcgcgccta
     7081 ctcaggcggt cgtcgtcccg atctggcgcg gcaagaacaa gggagaggtg cgccgcgagg
     7141 cagaggcgct gtacacggag ctcaaagact cgggtctcag gatggaggtc gacctcgacg
     7201 aggagcactc gcccggctgg aagttcaacg aacacgagct caggggcgtg cccgtgcgcg
     7261 tcgagctcgg tccgagggac atcgagaatg gacaggcggt gctggtgcgc cgcgacacgg
     7321 gggagaagga gttcgtggag aggaaggagc tgccccacag gctcaataca ttgatggtcg
     7381 agatacagga gaacatgctc cgtaatgccg agaccttccg cgaggagaat acccgcaggg
     7441 ccgagagcta cgaggagttc agggagatca tcgcccgcaa gcgtggcttc gtcgtcgcac
     7501 cctgggatgg tacggaggag acggagcaga agatcaaaga cgatacgaag gccacgatcc
     7561 ggctcctgcc cttcgagagg gaggaagggg aggacctcgt ctcaggcaag ccgggcaag
//
LOCUS       DATAIR010000462         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_398711, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000462 DATAIR010000000
VERSION     DATAIR010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398711"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VHM69_11215"
     CDS             <1..354
                     /locus_tag="VHM69_11215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2741007.1"
                     /translation="LGRLATVDAEGGPHVVPVGFRFNEELDTVDIGGHDLGATKKFRD
                     AGATGKAAFVVDDVLPPWRVRGVEIRGRAEVYSEGGSEVMEGFSEELIRIFPRRIVGW
                     GLDSGAFKANSRSVG"
     gene            381..812
                     /locus_tag="VHM69_11220"
     CDS             381..812
                     /locus_tag="VHM69_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2741008.1"
                     /translation="MKENTSFVLAKVCKAHRTSVGDLLAEYGLHVGQEMVLVELWQDD
                     GLRGGELAARLRVEPPTVTKMLRRLEMCGLIQRSQDPSDARSFRIHLTQKGRALEEPV
                     GRCWEKAEERTLAGLTVRERQIFRRLLNRVRSNLDPEFGVE"
     gene            1004..2446
                     /locus_tag="VHM69_11225"
     CDS             1004..2446
                     /locus_tag="VHM69_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741009.1"
                     /translation="MEVEARKSGGTSTRLLLAVVVTAILISVLNQTFVNVVVPDIQRD
                     YGATQGQVGWVITGYMLVFAVGIPLYGRIADLYSLRRTFVVGLAVFAAGSLFCALAPT
                     LPLLVAGRILQGIGASAIPALGFAAVAKALPPGERGTALGLLSASVGAGAAVGPVFGG
                     LLAGLFGWHVLFLFTVALSLALIPGAMRALPGASKAGAEQTDFAGVIRRFDVPGGLSL
                     ALSAGFALFAVTEGQVLGFSSPFSWGSFALSILLAVYFVWRTRTAPEPFVSPALFHNK
                     AFLATAAVGFFMMFANLGSLILAPLLLSEQNGLSAAGIGLVLAPGAIVVAVLSPLAGR
                     LSDRFGPRALVRTGLVIIMASILFISAYAAGASATWVAIGLLGQGIGFAAVNSPNANA
                     ATATLSPQESGVGLGIYQMIFFLGGGFGPAVAASFLAFRHEAGSSALIPLYTLDAAPF
                     SDAFLLVSAASIVALAASFGLRSTTGKEIS"
     gene            2443..3333
                     /locus_tag="VHM69_11230"
     CDS             2443..3333
                     /locus_tag="VHM69_11230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2741010.1"
                     /translation="MTDYGRDLEFGVSVVPLADPPDWAAQLAKAADRAGLDLVGIQDH
                     PYQRRFMDTWTLISTLVPITERVRFFPDVANLPLRPPAMLAKAAASLDVLSGGRVEMG
                     LGAGAFWDAVVGMGGPRRSPGEAVRSVEEAIQVMRFVWSDERSLRFDGEFYSLKGMRP
                     GPHPVHDIGIWVGAYGPRMLNLIGRLADGWVPSFGYSPPERLPEMQKRIDEGAAEAGR
                     EPKEIRRAYNLSGNIGTEGDGPLDGPAAKWIETLTGFALEHGMDTFIYWPSQDHERQV
                     EVFANEVVPAVREGVQAGRS"
     gene            3383..3700
                     /locus_tag="VHM69_11235"
     CDS             3383..3700
                     /locus_tag="VHM69_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741011.1"
                     /translation="MGKKHLKTHRRSAKPRPARQTAANLLSARSTRSILSFLSIGSIL
                     SAGSILSIGSAGSILSIGSAGSILSIGSTGSILSIGSVGSILGVGTVGSRPSRSEDAE
                     DEP"
BASE COUNT          651 a         1227 c         1346 g          727 t
ORIGIN      
        1 ctgggcaggc tggcgacggt ggatgctgag ggcgggccgc acgtcgtgcc cgtcgggttc
       61 aggttcaacg aggagcttga caccgtagac atcggcgggc acgacctcgg cgctaccaag
      121 aagtttcggg atgcaggggc cacggggaag gctgcgttcg tggtcgacga cgtgctaccg
      181 ccctggcggg tacgaggcgt cgagatccgt ggccgggccg aggtctattc ggagggtgga
      241 tcggaggtca tggagggctt ctcggaggag ttgatccgga tcttcccccg gcgcatcgtc
      301 ggctggggcc tcgactcggg cgctttcaag gccaacagcc ggtcggtcgg ctgatgcgcg
      361 gcgcggtccg agatcatccc gtgaaggaga acacgagctt tgttctggcg aaggtctgca
      421 aggcgcacag gacgagcgtc ggggacctgc tggccgaata cgggctgcac gttggccagg
      481 agatggtcct cgtggagctc tggcaggacg acgggctgcg tggtggtgag ctcgccgccc
      541 gtctccgcgt cgagcccccg accgtcacaa agatgctgcg gcggctggaa atgtgcggcc
      601 tcatccaacg cagccaggac ccttccgacg cccgcagctt ccgcatccac ctcacgcaga
      661 aggggcgcgc gctcgaagag cccgtcgggc gctgctggga gaaagccgaa gagaggacgc
      721 tggccggtct aaccgtcagg gagcgccaga tctttcgcag attgctgaac cgggtaagga
      781 gtaacctcga ccccgaattc ggggtcgagt agtaggatat ttcgacacta cagattttgg
      841 agatggtgcg tgtggcattt gcaaagggac gtttgaaggc cgtaggcggc acgcttcggg
      901 agggctcgaa cagcttcggc gcgctcgccg tagaactcgc tgagaagcta cgaacgaaag
      961 tgccttcgcg ccgagtggag atagccggag tgccggcttg agcgtggagg tagaggcaag
     1021 aaaatcgggg ggtacgagca cccggctgct tctggctgtc gtggtgacgg cgatcctcat
     1081 ctccgtcctc aaccagacgt tcgtgaatgt cgttgtgcct gacatccaga gagactacgg
     1141 ggcgacgcag gggcaggtgg gatgggtcat cacgggctac atgctcgtct tcgccgtcgg
     1201 catcccgctc tacgggcgca tcgccgatct gtatagtctg cgccgcacgt tcgttgtcgg
     1261 cctcgccgtt ttcgccgccg gttcgctgtt ctgcgcgctg gctcccaccc ttccgttgct
     1321 cgttgccgga cgcatacttc aggggatagg cgcctctgcg ataccggccc tggggttcgc
     1381 ggcggtggcg aaagcgctgc ccccgggcga acggggtacg gcgctcgggc ttctctccgc
     1441 cagcgtcggc gcgggcgccg cggtgggccc cgttttcggc ggccttctcg cgggcttgtt
     1501 cggatggcac gtgctgttct tgtttaccgt ggcgctttcg ctggccctca tccccggcgc
     1561 gatgcgcgcc ctgccgggcg cctcgaaggc gggcgcagag cagacagact ttgccggtgt
     1621 aatacggcgc ttcgacgtgc ctggcgggct ctcgctcgcg ctctccgcgg ggttcgcgct
     1681 cttcgccgtc acagagggac aggtgctcgg cttcagttcg cccttctcgt ggggaagctt
     1741 cgcgctctcg atcctgctgg cggtctactt cgtgtggcgg acccgcacgg cgcccgagcc
     1801 tttcgtctcg cccgcgctct ttcacaacaa ggcgttcctg gccacggctg cggtcgggtt
     1861 cttcatgatg ttcgccaacc tggggagcct gatcctggct ccactcctcc tctcagagca
     1921 gaacgggctc tccgcggccg gcatcgggct cgtgctcgcg ccgggcgcga tcgtggtggc
     1981 cgtactctcg cctctggcag gcaggctctc tgaccggttc ggcccgcggg cgctagtcag
     2041 gacgggactg gtcatcataa tggcttctat cctcttcatc tccgcctacg ccgcaggtgc
     2101 ctcggccacc tgggtggcaa ttggcctcct gggacagggc atcggcttcg cggcggtcaa
     2161 ctcgcccaac gccaacgcgg caaccgcgac gctctcgccg caggagagcg gcgtcgggct
     2221 ggggatctac cagatgatct tcttcctcgg cggcggcttc ggaccagcgg tcgcggccag
     2281 tttcctggcc ttcaggcacg aggcgggatc cagcgccctc atccccctct atactctgga
     2341 tgccgcgcca ttctccgacg cgttcctgct cgtgagcgcg gcctcgatcg tggccttggc
     2401 ggcctcgttc ggcctgcgga gtaccacagg gaaggagatt tcatgacaga ttacggcaga
     2461 gatctcgagt tcggcgtcag cgtggtgccg ctcgccgatc cgcccgactg ggccgcgcaa
     2521 ttagcgaagg cagcagatag agccggtctg gacctcgtgg ggatacagga ccatccctac
     2581 cagaggcgtt ttatggacac gtggaccctg atctcgactc tcgtcccgat caccgagcgc
     2641 gtccgcttct tccccgacgt ggcgaacctg cccctgcgtc cgccggccat gctcgccaag
     2701 gccgccgcga gcctcgacgt gctctctgga gggagggtcg agatgggcct cggagccggg
     2761 gcgttctggg acgccgtcgt cggcatgggc ggcccgcgca ggagccccgg cgaggccgtc
     2821 cgctccgtcg aggaggccat acaggtgatg cgattcgtgt ggagcgacga gcgttcgctg
     2881 cgcttcgatg gcgagttcta ttccttgaaa gggatgcgtc caggaccaca tccggtccac
     2941 gacataggca tctgggtcgg ggcctacggg cccaggatgt tgaacctgat cgggaggctc
     3001 gcagacggct gggtgccatc cttcggctac tcgcctcccg agcgactacc ggagatgcag
     3061 aagcggatag acgaaggagc ggcagaagcg ggcagggaac cgaaggagat ccgtcgcgcc
     3121 tacaacctct cgggcaatat cggaacggag ggcgacggtc cacttgacgg cccggccgcg
     3181 aaatggatcg agaccctcac gggcttcgcc ctggagcacg ggatggacac ctttatctac
     3241 tggccctctc aggaccacga aaggcaggtc gaggtcttcg ccaacgaggt agtgcccgcg
     3301 gtaagggaag gcgtccaggc agggcgttcc tgaggcgcgg tggtaaactg ccgtgaggtg
     3361 ggcgtcgtcc gtaggaggcg tgttgggaaa gaagcatctg aaaacgcacc gtaggtcggc
     3421 gaagccacgt ccggcccgcc aaaccgccgc caacctgctc agcgcgagga gcacgcgcag
     3481 catactcagc ttcctgagca tcggaagcat cctgagcgcc ggctccatcc tcagcatcgg
     3541 cagcgcagga tcgatcctga gcatcggcag cgcgggcagc atcctcagca tcggtagcac
     3601 gggttcgatt ctcagcatcg gcagcgtggg ctccatcctt ggcgtgggta ccgttggttc
     3661 ccgcccctcc cgaagtgaag acgcagagga tgaaccttag gcacgaaggg accacagact
     3721 tgggtcctca aagcagcttg cggaaggatt gatcctccca caagccgggc tacaggcaac
     3781 aggtttcagg cttcaggaag gtctttccgg agggacggtg gtcgctaagc tgcacctctg
     3841 gggcgaagtg gatcaatact tatgtactgc gctgttagag agaacggttt cgcacgaaca
     3901 tcttggaagc tcctgcacgc gatgttcgca gaagaccaca gccaacgccg g
//
LOCUS       DATAIR010000463         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_29190, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000463 DATAIR010000000
VERSION     DATAIR010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29190"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..138
                     /locus_tag="VHM69_11240"
     CDS             10..138
                     /locus_tag="VHM69_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2741012.1"
                     /translation="MCILEAMKMESEVRSQKAGTVSEIRIQAGQTVRSGDTLIVVE"
     gene            148..1209
                     /locus_tag="VHM69_11245"
     CDS             148..1209
                     /locus_tag="VHM69_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741013.1"
                     /translation="MAEQTALYEWRGEGDEAEIVLYAPDDTSFERALPAARLPGVESP
                     VYAAASQEGFGWSAASTTHVAPDLLSPPLRGLLLVAGTSSENLGVPVSELRDRVLRDL
                     PEAGSRLPSLKESAVGRLCESGARAAAEDGLIEEEDLPLLGVLPGDSDALGRRALAAG
                     PREWEGGASPDLRVITEVLDGEGADSLGLEFGTLAFVVRIGAGDLGRLALAGHTERIK
                     VRSEAPDPASATIGSGEYEDLVAAVRAAANFADARAARVLWMLRLVLGGVAGGLDIRA
                     AWRVGGVEERDGRLVHRRGLASVREGNVLVSGSYVAVGTGNMWSSAPPFGVFEEEGGW
                     PWEEAGLLERWVRLDPPEG"
     gene            1212..1478
                     /locus_tag="VHM69_11250"
     CDS             1212..1478
                     /locus_tag="VHM69_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741014.1"
                     /translation="MDLLMVRDRSTGRFVYTEQMERRPGETSWEYVRRSVRREARIRT
                     RFDGDAMEVIVGWDVDSVEDFLRAHPEYRHDGAGERLEGDTIDQ"
     gene            1468..1737
                     /locus_tag="VHM69_11255"
     CDS             1468..1737
                     /locus_tag="VHM69_11255"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HEX2741015.1"
                     /translation="MTSEVLDKFAQLITTALGLVAALAWNTAIQNLFNKVFGEAGARL
                     AGQFLYATLITIVVIFATIAVSRAAERAKKAEAEQNRGLFGRSRE"
     gene            complement(1795..2295)
                     /locus_tag="VHM69_11260"
     CDS             complement(1795..2295)
                     /locus_tag="VHM69_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2741016.1"
                     /translation="MSADGFTKMSIYGMNMDLFSSSPIVILKVEDENRYLPIWIGQAE
                     ARSILFKLQNQEFVRPLTHDLALSLVNELGGSMERITVTELRDSTFFATIRLEIEGKT
                     VEVDSRPSDAIALAIRSGAEIFASDEVIEEAGVVFEEAMEEAPEEEVVDKFKDWMNRV
                     SPEDFK"
     gene            2450..2523
                     /locus_tag="VHM69_11265"
     tRNA            2450..2523
                     /locus_tag="VHM69_11265"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2484..2486,aa:Ile,seq:gat)
     gene            3047..>5186
                     /locus_tag="VHM69_11270"
     CDS             3047..>5186
                     /locus_tag="VHM69_11270"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2741017.1"
                     /translation="MLDLAGWSVQDTKDLDFTASRGIALREFPLKAGFADYMLFVDRQ
                     AVGIVEAKKEGTPLSGVATQSRRYLDGLPPHVQRVGTPLPFAYESTGIETVFRDVRDP
                     DYRSRRVFSFHRPGTLLGWAREPETLRARLKEMPPLMTAGLRECQVEAIEGLERSLPE
                     NRPRSLIQMATGSGKTFTAVSFSYRLIKHAGARRVLFLVDRANLGRQTLKEFQQYATP
                     DDGRKFTELYNVQHLAGGWIDPVSKVCISTIQRLYSMLRGEELDESLDEASLNEITSL
                     SERPKEVTYNPQIPVETFDFVVVDECHRSIYNVWRQVLEYFDAFLIGLTATPSKQTFG
                     FFDQNLVMEYNHERAVADGVNVGYDVYRIKTEVGEQGGKVDAGFYVDYRDKATREVRW
                     GQLEEALEYTAQELDRSVVVRDQIRTVIRAYKERLFTDLFPGRTVVPKTLIFAKDDSH
                     AEDIVEIVREEFGKGEEFCKKITYRTQEKSETLISEFRTSPVLRIAVTVDMISTGTDV
                     KPLEVLIFMRDVKSQTYYEQMVGRGTRTISETDLQAVTGDASRKTHFVLIDAIGVTET
                     AKGTDVRPLERRPTVAFDKLLSMVQFGSRDPDTLTTLASRLSRLDRQLDEWNRQSIRD
                     ASGGKDPSELANALLDAVDPDKVRQQAAETSGTDSPTEAQIQEAAFALAERACGPFDS
                     PELRNTLTSLKRRSEQVIDRVSEDRVIYAGY"
BASE COUNT         1031 a         1521 c         1687 g          947 t
ORIGIN      
        1 gacgagcccg tgtgcatcct cgaggcgatg aagatggaga gcgaggtccg ttcccagaag
       61 gccggcaccg tctccgagat ccgtatccag gccggccaga cggtgcggtc aggggacacc
      121 ctgatcgtcg tagagtaagg ccgtactttg gccgagcaaa cggcgctcta cgagtggcgc
      181 ggcgagggcg atgaggctga gatcgtcctc tatgcccccg acgacacgtc cttcgagcgt
      241 gccttgcctg ccgccaggct ccccggcgtc gagagccccg tgtacgccgc ggcctcacag
      301 gagggcttcg gatggtccgc ggcctcgacg acgcacgtgg ccccggatct cctttcccct
      361 ccactgcgcg gcctgctcct cgtcgccggt acgagctccg agaatcttgg cgttccggtg
      421 agtgagttga gggaccgggt gttgcgcgac ctgcccgagg ccggctcacg gctaccgtct
      481 ctgaaagagt ccgcagtcgg gagactctgc gagtcgggag cgcgcgccgc cgcggaggat
      541 ggccttatcg aggaagagga tctgccactc ctgggcgtac tccctggcga ttcagacgcc
      601 ctcggcaggc gggccctcgc cgccggtccg cgtgaatggg agggtggagc aagccctgac
      661 ctcagggtaa ttacggaggt ccttgacggc gagggcgcgg atagcctggg gcttgagttt
      721 gggaccctgg cctttgtcgt caggataggc gcaggtgacc tcggcaggct cgcgctcgcg
      781 ggtcacacgg agcgcatcaa ggtcaggtcg gaggcccctg atccagcctc cgctactatc
      841 gggagcggag agtacgaaga cctcgtagcg gcggttcgcg ctgcggccaa cttcgctgac
      901 gcgcgcgccg ccagggtact ttggatgctg cggctggtgc tcggcggcgt ggccggagga
      961 ttggacatcc gggccgcctg gagggtcggc ggggtcgagg agcgggacgg gcggctcgtg
     1021 catcgcaggg gtctcgcctc ggtccgggaa ggaaacgtgc tcgtctctgg tagttacgtg
     1081 gccgtaggaa caggtaatat gtggtcgagc gcgccgcctt tcggggtttt tgaggaggaa
     1141 ggcgggtggc cgtgggaaga ggctgggttg ttggagcgtt gggtgaggtt ggatcccccg
     1201 gagggctgag gttggacctg ttgatggtgc gggaccggag tacgggccgg ttcgtatata
     1261 ccgagcagat ggagcgcagg cccggcgaga cgtcgtggga gtacgtccgg cgcagcgtca
     1321 ggagggaggc caggatccgg acgcgcttcg acggggacgc gatggaggtt atcgtcggat
     1381 gggacgtcga ctccgtggag gatttcctcc gtgcacaccc ggagtaccgc cacgacggag
     1441 ccggcgagcg actggaagga gatacgattg accagtgagg ttctggacaa gttcgcgcaa
     1501 ctcataacga cggccctcgg gctggtcgct gccctggcct ggaacacggc catccagaac
     1561 ctgttcaaca aggtcttcgg cgaggccgga gccaggctcg ccggacagtt tctctacgct
     1621 accctcataa ccatcgtggt catcttcgcg acgatcgccg tcagccgggc cgcggaaagg
     1681 gcgaagaaag ccgaagcgga gcaaaacaga gggctcttcg gtcgttcaag ggagtagaag
     1741 ctccgctggc agtccctgaa acctaatacc tgaagccgcg gcccgtaggg ccgcttactt
     1801 gaagtcttcc ggggagaccc tgttcatcca gtccttgaac ttgtcgacga cctcttcctc
     1861 cggggcctcc tccatcgcct cctcgaagac gacgccggcc tcctctatca cctcgtccga
     1921 ggcgaagatc tcagcccccg agcggatggc gagggctatg gcgtcggatg gcctcgagtc
     1981 cacctcgacg gtctttccct caatctccag cctaatggtc gcgaagaagg tcgagtccct
     2041 gagctcggtc accgtgatcc tctccatcga gccgccgagc tcgttgacga ggctcagtgc
     2101 gaggtcgtgt gtcaggggcc tgacgaactc ctggttctga agcttgaaca gaatagagcg
     2161 tgcctcagcc tggccgatcc agatcggcaa ataccggttc tcgtcttcga ccttgaggat
     2221 gacgatgggg ctggaggaaa agagatccat gttcatgccg tagatggaca tcttggtgaa
     2281 gccgtcggcg ctcactggct atatgctcct ctcactccgc gaggtcgcgg ggattatcct
     2341 atacgagccg attttacttc ccgcctctcc ggtgttcaat caggttgtca gcagcctgcg
     2401 cgtgttgtac attattccgg cggtgcggat ctgttgcacc gatcacttag ggcctgtagc
     2461 tcagccggtt agagcgcttc tctgataagg aagaggtccc tggttcgagt ccaggcaggc
     2521 ccacatcaag aaaacgtcgt ttgcaggtaa aaaacgagaa acgagcgaag gccacggata
     2581 cgctctgggg cctttgtgca gcaaccgtgc agtaacgcag cgtagctgtg tgctaatctg
     2641 cctcgaatga gaccttcaaa ctatacgctg gtgacctgcg tcctaacgtc agctggcgta
     2701 tgcatccgca gacacaccgg gttgggggcg caagctacaa cacgggagag gtagtgattc
     2761 aggtagcaca ctcgttcggc ggcagcgcct accagacgag caccgcagac gaagggcgaa
     2821 tcgaaggcat ccccagatgg ttctagaacc atctggggat gagcccatac cgtagcatct
     2881 cattgtctta ccgagcgaac ggggcagaca tcctaccagc tgccccgttc gcccttgctg
     2941 aaaatgtagc gcctgaaccc gtacctgact aaaatgcgag catcacacgg atcttggggg
     3001 acaattgact acacccgagc gacttgccag acataacatt gatcggatgc tcgatctcgc
     3061 agggtggagt gttcaggata cgaaagacct ggacttcact gcatcgaggg gtattgctct
     3121 gcgggagttt cccctcaagg cgggatttgc agactacatg ctcttcgttg acagacaggc
     3181 cgtgggcatt gtcgaggcca agaaggaggg cacgcctctt agcggagtag ctacccagtc
     3241 caggaggtac ctcgacgggc ttccgccaca cgtccagcga gttgggacac cgctaccttt
     3301 cgcctacgag agcacgggca ttgagacggt gttcagggac gtgcgagatc ctgactaccg
     3361 ctcccgtcgg gttttttcgt ttcacagacc tgggacgctt ttgggatggg cacgagaacc
     3421 ggagacgctg cgggcacgcc tgaaggagat gccgcctctc atgactgccg ggctgcgaga
     3481 gtgtcaggtc gaggcaatcg aagggctcga gcgctcccta cctgagaaca ggccgcgctc
     3541 gctgatccag atggctactg gaagcggcaa gacgttcacg gcggttagct tctcctaccg
     3601 gctcatcaag cacgccggag caagacgggt tctgttcctg gttgaccggg cgaacctggg
     3661 acggcagacc ttgaaggagt ttcagcagta cgcaacccca gacgatgggc ggaagttcac
     3721 cgagctctac aacgtgcagc accttgcggg aggttggatc gaccctgtga gcaaggtctg
     3781 catctctacc atacagcgcc tctactcgat gctcagggga gaggagctgg acgagtcgct
     3841 cgatgaggct tcactcaacg agatcacgtc cctaagcgag cggccgaagg aggttaccta
     3901 caatccgcag ataccagtag agaccttcga cttcgtggtc gtggacgagt gccaccgctc
     3961 catctacaac gtctggcgtc aggtcctgga gtacttcgac gccttcctga tcgggctcac
     4021 ggcgacgcct tccaagcaga ccttcgggtt cttcgaccag aacctggtta tggagtacaa
     4081 ccacgagcgg gcggtggcag acggggttaa cgtgggctac gacgtgtatc ggatcaaaac
     4141 tgaggtcgga gagcagggcg gtaaggtgga tgcgggcttc tatgtggact atagggacaa
     4201 ggcgacccgc gaggtgcgct ggggtcagct ggaggaggcg ctagagtaca cggcgcaaga
     4261 gctggaccgt agcgtcgtcg tccgagacca gatccgaacc gtgatccggg cctacaagga
     4321 gaggctcttc accgacctct tccctggccg cacggttgtt cccaaaactc ttatcttcgc
     4381 caaagacgac tcccacgcag aagacatagt ggagatcgtg cgagaggagt tcggtaaggg
     4441 tgaggagttc tgcaagaaga tcacctacag gacgcaggag aagtcggaga ctttgatctc
     4501 cgagtttcgg acctccccgg tcctgcggat agccgtaacg gtggacatga tctcgaccgg
     4561 caccgacgtg aagccgctcg aagtactcat cttcatgcgc gacgtgaaga gccagaccta
     4621 ctacgagcag atggtagggc gcggcaccag gaccatctcg gaaacagacc tccaggcggt
     4681 cacaggggat gcgagccgca agacccactt cgtcctgata gacgccatag gggtaactga
     4741 gacagccaag ggcacggacg tgcgtcctct ggagcgcagg ccgaccgtcg ccttcgacaa
     4801 gctcctctcg atggtccaat tcggtagccg cgaccccgac acgctcacca ccctcgcctc
     4861 gaggctctcc cgtctcgacc ggcaactcga cgagtggaac cgccagagca tccgtgacgc
     4921 ttcgggcggc aaggacccat ctgagcttgc caacgccctg ctcgacgccg ttgaccctga
     4981 caaggtgcga cagcaggcag cggagacgag cggcacggac tccccgacgg aggcccagat
     5041 acaggaggcc gccttcgcgc tcgcggagag ggcctgcggg cccttcgact cgccggagct
     5101 caggaacacg ctaacgagcc tcaagcgccg cagcgaacaa gtcatagacc gggtgagcga
     5161 ggacagggtc atctacgcag gctacg
//
LOCUS       DATAIR010000464         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_152181, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000464 DATAIR010000000
VERSION     DATAIR010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152181"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VHM69_11275"
     CDS             <1..1403
                     /locus_tag="VHM69_11275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX2741018.1"
                     /translation="DFDVFVQDLPDGEPRMVWEVSGSHMVADWVPDGSALIISRHHSN
                     VNNDLYRLDLTTGEATLLTPHQGDARFSGANVTPDGGSVYLATDRDGEFLRLARLDLA
                     TLSLDYLTPDDWDVEGVELSRDGRYLVTSRNVDGYSDVLLFNGRGRRMPDPRIPEGIV
                     GGFEFSPDSREVAFTLTAPERNPDLWVLDLPDGDARRITRSSTAGIPPRTFRRPRVVR
                     YPSFDGRQIPALFHEPGIENAPVIVNVHGGPESQSRPAFAPVTQYLLHRGYAVFFPNV
                     RGSTGYGKTYTHLDDVQLRMDSVKDLAHAAYWLGEQGYELVAVMGGSYGGFMVLAALT
                     EYPELWTAGVDIVGIANLVTFLENTGSYRRSLREPEYGSLEKDRAFLESISPIHKAEN
                     IKAPLMVVHGKNDPRVPVGEAEQIVDRVKTNGGTVEYLLYEDEGHGLARLKNRLDAYP
                     RIAVFLDEHLAGRRLG"
     gene            1750..3261
                     /locus_tag="VHM69_11280"
     CDS             1750..3261
                     /locus_tag="VHM69_11280"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX2741019.1"
                     /translation="MSISGNWDRRLSRRRFLGIGGLSAAALVLGSRGAGAQSSTSVGF
                     GDLVSDPGGLIDLPPGFQYRVISEKGSPLSSGGTVPGDHDGMAAFPGPSANTTVLVRN
                     HELNSNDPAPVVGTNPYDPSQTGGTTGILVNLDDRTEVRDFVTSSGTRNNCAGGATPW
                     GTWLTCEEDRTTNHGYVFEVNPNDPENGLSKKPIRAMGFFSHEAVDIDPATGIAYLTE
                     DDFRGSIPSDPNAEVIADDAPPGSRVSFLYRFIPSVKPTKPGDLQKGGKLQVLTLEQL
                     NFNADLARPGQTFGVVWKDVNPEEPHEGAEDVGAARFGRLEGAHFSGGAFWFDDTIGG
                     EKRLGQIFRLIPRGGSGNTGDVLELFYEGTKPGNMESPDNIIVTPWGDLWFAEDEAVS
                     GDSVNRVMGITPGGQVYPFARNPRSDSEFAGPTFSPDGSTFFVNMQGDGLTFAIWGPL
                     PGSNAARQRQMAVAAPPKSMAPKVSGELAEAAQKFGLSNLEAAAYESLGVSLA"
     gene            3368..4384
                     /locus_tag="VHM69_11285"
     CDS             3368..4384
                     /locus_tag="VHM69_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX2741020.1"
                     /translation="MPTRASGGMLHGGAKLRRFGLLAALIFGTMILAATAAGATPSGK
                     TSQANAPVLNIGHRGASGYAPEHTIPAYDLALEQGADYIEIDLQMTKDGVLVAMHDDT
                     LDRTAQAPPGVPERFCSGPVIKRTLEQIRMCDAGSWFNETYPQYANPEYAGLQIPTLE
                     EIFQRYGTGVNYYIETKNPEAAPGMEEELLRLMEKYGLMEPAAQNWQVLIQSFSPASL
                     QKIHALEPSLPLIQLFSSTETSQTIQAQLDLVRTYAVGIGPSKSDVDAALVEAAHARC
                     LDIHPYTVNETAEMQTLISLGVDGMFTNFPDRLDALLGKDAAPGMTGAKLAAEASEVC
                     RAGN"
     gene            4400..>5212
                     /locus_tag="VHM69_11290"
     CDS             4400..>5212
                     /locus_tag="VHM69_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741021.1"
                     /translation="MSNRSVVLIVAVLSAMLVTVPVLAAPQQSGDVTRTTFVAKKGGE
                     ALLDLTASAPGTDWGKSGSESAVVTVTLDGKYNQDVVLFAGEQPFTYQLALGTVGAGR
                     HTVEVAFNKEKSPAGATGAQIQSLSTQVVSAKDPASLAYQYSPILYGRDLPEIPGQYE
                     NNYTDVPLIMYHTTSTDPASGNTTIEYTVIWSNEDGGTNTPALMARWGRSTDIEWIYR
                     VTLDPQHKIVSEYYQAPNHATLPFTGAKEGNHPLLQTSTSNNNLTQVTDPALS"
BASE COUNT         1075 a         1698 c         1574 g          865 t
ORIGIN      
        1 cggacttcga cgtcttcgta caggacctgc cggacgggga gccgcggatg gtgtgggagg
       61 tctcaggctc ccacatggtt gcggactggg tccctgacgg gagcgccctg atcatctccc
      121 gccaccactc caacgtcaac aacgacctgt accgcctgga tctcacaacc ggcgaggcca
      181 cgctgctgac gccgcaccag ggggacgcgc gtttctccgg cgcgaacgtg actcccgacg
      241 gcgggagcgt ctacctggct acggaccgcg acggggagtt cctgcgcctc gcccgcctcg
      301 acctcgcgac gctgtcgctc gactatctca ccccggacga ttgggacgtc gagggcgtcg
      361 agctttcgag ggacggtcgt tacctcgtca ccagccgcaa cgtggacggc tactccgacg
      421 ttttgctctt caacggcagg ggacgcagga tgcctgaccc gcgcatacct gaaggcatcg
      481 tcggcggctt cgagttctca cccgattcca gagaggtcgc cttcaccctc accgcccctg
      541 agcgtaaccc ggacctctgg gtcctcgacc tgccggacgg cgacgcgagg cgcatcaccc
      601 gctcatcgac ggcgggtatt ccgccgcgca ccttccgcag accgcgggtc gtgcgctatc
      661 ccagcttcga cgggcgtcag ataccggctc tcttccatga gccggggata gagaacgcgc
      721 ctgtgatcgt caacgtgcac ggcgggcccg aaagccagtc gcgccccgcg ttcgcccctg
      781 ttacgcagta cctgctgcac aggggctacg ctgtgttctt cccgaacgtc cgcggatcga
      841 ctggctacgg caagacttat acccacctcg atgacgttca gttgcggatg gattccgtca
      901 aggacctggc gcacgcggcg tactggctgg gggagcaagg atacgagctc gtcgctgtca
      961 tgggcggctc ctacggagga ttcatggtgc tcgccgctct caccgaatat cccgagctat
     1021 ggacggcggg cgtggacatc gtggggatcg ccaacctggt cacgtttctg gagaacacgg
     1081 gcagctaccg tcgaagcctg cgtgagccag agtacggctc tctggaaaaa gaccgcgcgt
     1141 tcctcgagtc catcagcccc atccacaagg cagagaacat caaagcccca ctgatggtcg
     1201 tccacggcaa gaacgacccc agagttccgg taggagaggc agagcagatc gtggaccgcg
     1261 tcaaaaccaa cggcggtacg gtcgagtacc tgctatacga ggacgaggga cacggcctcg
     1321 ccaggctaaa gaaccgcctc gacgcctacc ccaggatcgc agtcttcctc gacgagcatc
     1381 tcgcgggacg ccggctcggt tgacactggt ttcgtgtaaa tgtaagctct gttccagact
     1441 tatagagagg gaaggggacg tcgcatacgt tcatcgggcg ggtcctgcta tgattccagg
     1501 cctcccttcg cattcagaaa cagaagcacg ccgcgtccgc tggcttggtg ttctctgctc
     1561 actgtaacaa cagcgtcgta gtagtagatc atcatggtta atgcgagcgg aagcacgtaa
     1621 tgtgctctcg ctcgtcgttc ccgctgagca gaggagcttt tacagaaggt ttacacgtgt
     1681 gtaacaaagc cgggtgaata tgctggtata tagctctcgg agactgaaga tcaaacggag
     1741 gtgggcactt tgagcatcag cggcaactgg gaccggcgtt tgagccggag aagatttctt
     1801 ggcataggag gcctcagcgc ggcggcgctg gtactcggct ccagaggcgc gggggcccag
     1861 agcagcactt cggttggttt cggtgacctt gtctcggacc ccggaggctt gatcgacttg
     1921 cctccgggtt tccagtaccg tgtgatctcg gagaagggtt cccctctctc gagtggcggc
     1981 accgttccgg gagaccatga cggaatggcc gctttccccg gcccgagcgc gaacaccacg
     2041 gtcctcgtcc gtaatcacga gctgaattcg aacgaccccg caccggtcgt cggcacgaac
     2101 ccctatgatc cttcgcagac cggtgggacg acgggcatcc tcgtgaacct ggatgaccgc
     2161 acggaggtaa gagatttcgt cacctcatcg gggacgcgca acaactgcgc gggcggcgcg
     2221 acgccttggg ggacctggct gacctgtgaa gaggaccgca caacgaacca cggctacgtc
     2281 ttcgaggtca acccgaacga ccctgagaac gggctctcca aaaagccgat cagggctatg
     2341 ggcttcttct cacatgaggc cgtggacata gatccagcga caggcatcgc ctacctcacg
     2401 gaggacgact tccgcggcag catcccctca gatccgaacg ccgaggtcat cgcggacgac
     2461 gcacccccag gaagccgcgt cagcttcctc taccgcttca ttccgagtgt aaagcccacg
     2521 aagccgggcg atctgcagaa gggcggcaag ctccaggttc tgacgctcga acagctcaac
     2581 ttcaacgcag acctcgccag accgggacag acgttcggcg tggtctggaa ggacgtaaac
     2641 ccggaggagc cgcacgaggg cgccgaggac gtaggcgccg cgcggttcgg ccgcctggag
     2701 ggcgcgcact tctccggcgg ggcgttctgg ttcgacgaca cgataggagg cgagaagcgg
     2761 ctaggccaga tcttccgtct catcccccgc ggtggctccg gtaataccgg agatgtcctc
     2821 gagctcttct acgaaggcac caagccgggc aacatggaga gccctgacaa catcatcgtg
     2881 acgccctggg gagacctgtg gttcgccgag gacgaggccg tgtccggaga cagcgtgaac
     2941 cgcgtcatgg gcatcacgcc aggcgggcag gtgtacccgt tcgcccgtaa tcccaggagc
     3001 gactccgagt tcgcaggccc gaccttctcc cccgacggca gtaccttctt cgtcaacatg
     3061 cagggagatg gcctgacctt cgcaatctgg ggcccgctcc cagggagcaa cgccgcgcgc
     3121 cagcgccaga tggccgtcgc agcgcccccg aagtccatgg caccgaaagt ctcgggcgag
     3181 ctcgccgagg ccgcccagaa gttcggcctg agcaacctcg aagccgcggc ctacgagagc
     3241 ctcggcgtat cgctggcgta gagggactcc ggacaacacc ttcagagtaa tccgcagtgg
     3301 gacccgcgtc cgagcaggtc ccacctgtct atccggccac gatgtcagga aaggggagga
     3361 aaggtccatg ccgacaagag cttctggagg gatgctacat ggtggagcga aacttcgacg
     3421 gttcggactc ctggctgccc taatattcgg tacgatgatc ctcgcagcca ccgccgcagg
     3481 agccacgccc tccggcaaga cttcccaggc caacgcgccg gtcttgaaca tcggtcacag
     3541 gggagcttct ggatacgccc ccgagcacac cattccagct tatgacctgg cgctcgagca
     3601 aggcgccgac tacatcgaga tagaccttca gatgaccaag gacggcgtgc tcgtagccat
     3661 gcacgacgac acactcgacc gcaccgcaca ggcgccgccg ggagtgcccg agcggttctg
     3721 ctcaggtccc gtcatcaaga ggacgctcga gcagataagg atgtgcgacg ccggaagctg
     3781 gttcaacgaa acgtatccgc agtacgccaa cccggagtac gcggggttgc aaatccccac
     3841 cctcgaagaa atcttccagc gctacggcac gggcgtcaac tactacatcg agaccaagaa
     3901 ccccgaagcg gctccaggca tggaggagga gctgctgcgc ctcatggaga agtacggtct
     3961 catggagccg gcagcccaga actggcaggt cctgatccag tccttcagcc cggcgagcct
     4021 gcagaagatc cacgccctcg aaccctcgct gcccctgata cagctcttct cgagcaccga
     4081 gacgagccag acgatacagg cgcaactcga cctggtgcgg acctacgccg tcggcattgg
     4141 cccgtcgaag tcggacgttg acgccgcgct cgtcgaggcg gcgcacgccc gttgcctgga
     4201 catccaccca tacacggtca acgagacagc cgagatgcaa accctgatct ctctcggcgt
     4261 cgacggcatg ttcaccaact ttcccgaccg gctcgatgcg ctgctcggca aggatgccgc
     4321 gcctggcatg accggcgcaa agcttgccgc tgaagcgtca gaggtttgcc gcgccggaaa
     4381 ttagaagaag gagcgtatga tgagcaatag aagcgtagta ttaatcgtcg cggtactctc
     4441 agcaatgctg gtgacggtac ctgtcctcgc cgcgccgcag caatcagggg acgtaaccag
     4501 gaccaccttc gtcgccaaaa agggcggcga ggcgttgctc gacctcacgg cctcagcgcc
     4561 cgggacggac tggggcaagt caggcagcga gtcggctgtt gtcaccgtca cgctcgacgg
     4621 caagtacaac caggacgtcg tgctcttcgc aggggaacaa ccattcacct accagctcgc
     4681 gctcgggacc gtcggtgcgg ggcggcacac ggtagaggtc gccttcaaca aggagaaatc
     4741 acccgctggt gccacgggag cgcagatcca gagcctctcc acacaggtcg tctccgcgaa
     4801 ggaccccgca tcactcgcgt accagtattc gcctatcctc tacgggcgcg atctccccga
     4861 gataccgggg cagtacgaga acaactacac agatgtgccc ctgatcatgt accacacgac
     4921 gagcaccgac cctgcgagcg gcaacacaac tatcgagtac acggtgatct ggtccaacga
     4981 ggacggcggg acgaacaccc ctgccctgat ggcccgctgg ggacgctcga cggatatcga
     5041 gtggatctac cgcgtaaccc tcgacccgca acacaagata gtctccgagt actatcaggc
     5101 acccaaccac gccacacttc cgttcacagg cgcgaaggaa ggcaaccacc cgctgctcca
     5161 gacctcgacc tcgaacaaca acctgacaca ggtcacggac cctgccctca gt
//
LOCUS       DATAIR010000465         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_398803, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000465 DATAIR010000000
VERSION     DATAIR010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398803"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VHM69_11295"
     CDS             complement(<1..515)
                     /locus_tag="VHM69_11295"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2741022.1"
                     /translation="MSTSVETTSEIRPFHVEIPEEDLAELRRRIEATRWLSKELVEDR
                     SQGVQLATMQALARYWATDYDWRKAEAKLNALPQFTTEIDGVDIHFIHVRSQHEDALP
                     LIMTHGWPGSVIELLETIGPLTDPTAHGGNAGDAFHLVLPSLPGYGFSSEPAELGWDA
                     ARTGRAWAQLM"
     gene            complement(652..1377)
                     /locus_tag="VHM69_11300"
     CDS             complement(652..1377)
                     /locus_tag="VHM69_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741023.1"
                     /translation="MSSFTLNSERFWERLWRSSGIIFIVFFVLSYVMYGLQPKVGASA
                     DTLVSFYDGNSTRILMATLMFGSSVLFLLWFAAAIRSMLHDAGQGGWGAAATASSAAL
                     GGMLWVLIAAGAALAYSIAGSGNDALTTGLNDLVWACIVVSSFPRAMLIMAGTFGLWR
                     AKLISNALFGAGVAAVLLVLVGGTTWASSGIWAPSGDYTRFVSPIIGVVWVLVFSRVL
                     LARRSPSTAPHSPEPPAAPAPAP"
BASE COUNT          289 a          490 c          473 g          255 t
ORIGIN      
        1 ctcatcagct gtgcccacgc gcgtccggtg cgggcggcgt cccaaccgag ctcggccggc
       61 tcactggaga atccgtaacc gggcaatgac ggcagcacca ggtgaaacgc gtccccggcg
      121 ttgccgccgt gagcggtcgg gtccgtcagc gggccgatcg tctcgagcag ctcgatgacc
      181 gatccaggcc agccgtgggt catgatcaac ggcaacgcgt cctcgtgctg ggagcgcacg
      241 tggatgaagt ggatgtcgac cccgtcgatc tcggtcgtga actggggcaa ggcgttcagt
      301 ttcgcctcgg ccttgcgcca gtcgtagtcg gtcgcccagt agcgcgcgag cgcctgcatc
      361 gtcgccaact gcactccctg cgaccgatct tcgacgagct ccttgctgag ccaccgtgtc
      421 gcctcgatgc gtcggcgcag ctcagcgaga tcctcttccg ggatctcgac gtggaagggg
      481 cgtatctcgc tcgtagtctc gacactggtg ctcatgacga cctccgtttc ttggtggggc
      541 aggattaggc cgacccctcc tccgtggttg ggggacttgc ggtgttggtt gcctcaggta
      601 tgggcgctcc tttcgtgaga gagatggagc gcggccccgc agcaccgcga actacggtgc
      661 cggcgccggt gccgccggtg gctcgggcga gtgcggggcg gtggagggac ttcttcgggc
      721 caagaggacc cggctgaaga ccaggaccca cacaacgccg atgatgggcg agacgaacct
      781 cgtatagtca ccggacggtg cccagatccc gctgctcgcc cacgtcgtgc cgcccaccag
      841 gacgagcagg accgccgcga ccccggcccc gaagagcgcg ttcgaaatca gcttcgcccg
      901 ccataacccg aacgtcccgg ccatgatcag catcgcgcgt gggaaggagc tcaccacgat
      961 gcaggcccat accaggtcgt tcagtccggt cgtgagcgcg tcgtttccgg agcctgcgat
     1021 cgagtacgcc agggctgctc ccgctgcgat tagcacccag agcatccccc cgagcgccgc
     1081 gctggaggcg gtcgccgccg ccccccaacc gccttgcccg gcgtcatgca acatgctcct
     1141 gatcgccgct gcgaaccaga gcaggaagag gaccgacgaa ccgaacatta gcgtagccat
     1201 cagtatccgg gtgctattac cgtcgtaaaa cgagacgagc gtgtcggccg acgcaccgac
     1261 cttcggctga aggccgtaca tgacgtaaga gaggacgaag aagacgataa agatgatgcc
     1321 gctacttcgc cacaagcgtt cccaaaagcg ttccgaattc agagtgaatg aactcataac
     1381 gatctccttt ccctggaccg caccacttcg gggtaacctg gagcctacca ggggagcgta
     1441 gtgagaacgt agtgaggagc cacaagcgtt cccaattcac agacgaaact gaattagtac
     1501 acttcaa
//
LOCUS       DATAIR010000466         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_244384, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000466 DATAIR010000000
VERSION     DATAIR010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244384"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VHM69_11305"
     CDS             <1..238
                     /locus_tag="VHM69_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741024.1"
                     /translation="TLRNPIEYGSLSEVGGVQRVVPEGRNLIFHCDERPGMVADVVAG
                     IVNAGGDIVNLSVQRPNLEDVFVKLTGEALRDEA"
     gene            228..950
                     /locus_tag="VHM69_11310"
     CDS             228..950
                     /locus_tag="VHM69_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2741025.1"
                     /translation="MRLDRPDSYWKKDVALFFSRRFALLIKMLYPVAIIVPVAASGMP
                     AKYAAAVIGIVAIMAGTFGAGESLTIDLNDGILVRVALTPLSPYRIVFEVLAVNAVLD
                     LVQLLPALLIVYLLHPVSIVWMLAATLAVFATLVTANCIGVFIANFTSSPADVLLYAT
                     AILFPLIYLSGFFRNQHPTGILQTLRGFVPFAYTDDAMKTVFGLTTGTTPLEILIYPT
                     IVCAVLACAVCMTAPRLLSQRP"
     gene            1149..>1712
                     /locus_tag="VHM69_11315"
     CDS             1149..>1712
                     /locus_tag="VHM69_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX2741026.1"
                     /translation="MSDSLRRYVIWVVVALVGAVCWGMLALSRGETINAGWLLFAALA
                     SYAIGYRFYARFIQRRVLETDDSRATPAERLNNGRDFEPMDRRVLFGHHFAAIAGAGP
                     LVGPVLAAQMGFLPGTIWIIVGVILAGAVQDMTTLFFSLRRDGKSLGQMAREEIGPFG
                     GAAALIAVLSIMVILLAVLALVVVLALA"
BASE COUNT          274 a          560 c          514 g          364 t
ORIGIN      
        1 gacgcttcgc aaccccattg agtacggttc cttgtcggag gtcggcggcg tccagcgcgt
       61 cgtgcctgag gggcggaact tgatcttcca ctgcgacgaa cgtccaggca tggttgccga
      121 cgtggtggct ggcatcgtca atgccggcgg ggacatcgtg aacctctcgg tccagaggcc
      181 aaacctcgag gacgttttcg tcaagctgac aggggaggcg ctgcgcgatg aggcttgaca
      241 ggccggactc ctactggaaa aaggacgtgg cgctcttctt ctccaggagg ttcgcgcttc
      301 tcataaagat gctctacccc gtagcgataa tcgtgcccgt ggccgctagc ggcatgcctg
      361 cgaagtacgc cgccgccgtc ataggcatcg ttgcgataat ggctgggacc ttcggggccg
      421 gcgagagcct cacaatagac ctgaacgacg gcatcctggt gcgcgtcgcg ctgacaccgc
      481 tctctccata caggatcgtc ttcgaggtcc tcgccgtcaa cgctgtgttg gaccttgtgc
      541 agctacttcc cgcgctcctg atcgtctacc ttttgcatcc cgtctccatc gtgtggatgc
      601 ttgctgctac gctcgccgtc ttcgcgacgc tcgtgacggc gaactgcatc ggggtattca
      661 tcgccaactt cacctcctcg ccggccgacg tgctcctgta cgcgacggcg attctgttcc
      721 ccctgatcta cctctcaggc ttcttccgca accagcaccc gacgggcatt ctccagacgc
      781 tccggggctt cgtccccttc gcctacacgg acgacgccat gaagacggtc ttcggactga
      841 ccacgggcac gacgccgctc gagatcctca tctaccccac catcgtctgc gccgtcctgg
      901 cctgcgcggt ctgcatgacc gcgccacgtc tcctctccca acgtccgtag agaccgtcga
      961 caatagcccc ctgtcgttga ggcgtggagg agacaaaccg gagcggtagc atcctccggt
     1021 tcgccggtac aaggcggtct tctccaatct ggcagcgggt tcccgcgttc aatacgctat
     1081 tccgttatga taggatgcat acgcttaggt aagagcctca gggaagggca ctttaggtag
     1141 gaggacctat gagtgatagt ctgcgtcggt acgtgatctg ggtagtcgtc gccctcgtcg
     1201 gggcggtctg ctgggggatg cttgccctct cacgcggtga gaccatcaac gcagggtggc
     1261 tcctcttcgc ggctctcgcc tcctatgcca tcggctacag gttctacgcg cgctttatcc
     1321 agagaagggt tctggagacg gatgactcgc gggcgacgcc tgccgagcgg ctcaacaacg
     1381 gacgcgactt cgagccaatg gacaggcggg tgctcttcgg gcaccacttc gccgccatag
     1441 cgggcgccgg accgctcgtc gggcccgttc tggccgcgca gatgggcttt ttgcctggca
     1501 cgatctggat catcgtcggc gtcatactgg caggcgcagt tcaggacatg actacgctct
     1561 tcttctccct gcgccgcgac ggcaagtccc tcgggcagat ggcgagggag gagatcggac
     1621 ccttcggcgg ggcggcggcg ctcatcgcgg tcctttcaat catggtcatc cttctcgccg
     1681 tgctcgccct cgtcgtcgtc ctcgcgctcg cc
//
LOCUS       DATAIR010000467         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_306589, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000467 DATAIR010000000
VERSION     DATAIR010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306589"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VHM69_11320"
     CDS             <1..1606
                     /locus_tag="VHM69_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX2741027.1"
                     /translation="LIVGLLVAVAALATLATRLRIPYPILLVLGGSALGFLPKLPPVE
                     LDPELVFLLFLPPLLYVSALFTSWRDFRANVRPISLLSVGLVLMTTFVVAAVVHAVIG
                     LPWAAAFVLGAIVSPTDAIAATAVAERLGVPRRIVTILEGESLVNDATGIVAYRIAVA
                     AVTTGAFSIWEAGLQFVLGAAGGVAVGLAVGWFIIWARRHLSEDPSIQNTVSLLTPFV
                     SYLLAEELPSSLWHYAGLPGEFHFSGVLAVVTTGLYWGRKAPTVISSGTRLQGYAVWE
                     LVTFLLNGLIFVLIGLQLRNVVEELGEYSAGELILYAVLVSLTVILVRILWVFPATYL
                     PRRVSRRIRERDPAPPWQAVTVIGWTGMRGVISLAAALALPLQTASGAEFVWRDLIIF
                     LTFCVILATLVLQGLSLPALIRALGLEDDRIGDREETHGRIGVAEAALERLEELVEEE
                     WVREDTAERTRALYNYRRNRFATRFEGDTDGVEERSGAYQRLMAELLGAQRKRLVRMR
                     DEGLIGDEAMHRIERDLDLEESRLEI"
     gene            1623..1955
                     /locus_tag="VHM69_11325"
     CDS             1623..1955
                     /locus_tag="VHM69_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2741028.1"
                     /translation="MVANITREELKAKMDRGDDFVLVEALSHAHYASSHLPGAINFPY
                     EFVDEAEKILPDKDTEIVVYCMNRDCSASREEARELDEMGYARVLHYADGKQDWIRAG
                     LPVERSGR"
     gene            1961..>2988
                     /locus_tag="VHM69_11330"
     CDS             1961..>2988
                     /locus_tag="VHM69_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2741029.1"
                     /translation="MSELLAGFALVAVVLIVAGLTSGFVERAPLSFPVIFLCLGFLLG
                     GLGMLDFHPESAVLEGVATLTLSLVLCLEAVRIGNIGMGGVGFVPILSLGPGTVVIVA
                     VIAAGSYFLLDVSAIEALLLGTILASTDPVVLRDVVREKRIPASVRQALNIEAGTNDI
                     VVLPTLLILIALANAAASGAAGWARLVGEVLILGPVVGFAIGAGAAWLMSHADRRYAI
                     NEVYQSLYGVGIVLLSYASATALGGDGFLAAFAAGFAIAVLNFDLCQCFLDYGETTSE
                     MAMLLSFILFGVVLSDLFSEVPLIPTLALAAIVIFVARPLAIGIVLRKAAVSNAARAF
                     IGWFGPRGL"
BASE COUNT          433 a          980 c          977 g          598 t
ORIGIN      
        1 gttaatcgtc ggccttctcg tggcggtggc ggcgctggcc acgctggcga cgcggctgag
       61 gataccgtac ccgatcctgc tcgttctcgg cgggtcggcg ctcgggttct tgccgaagct
      121 tccgccggta gagctcgacc ccgaactggt attcctccta ttcctgccac cgctgcttta
      181 cgtctccgcg ctgtttactt cgtggcgaga tttcagggcc aacgtcaggc cgatctcgct
      241 tctatcagtc gggctcgttc tgatgacgac cttcgttgtc gccgccgttg tccacgccgt
      301 catcgggctg ccgtgggcgg cggcgttcgt cctcggggcc atcgtctccc ccaccgatgc
      361 catcgccgcc accgcggtcg ccgaacgcct cggcgtcccc cgccgcatcg tgacgatcct
      421 cgaaggcgag agcctggtca acgacgccac gggcatcgtc gcctaccgca tcgccgtcgc
      481 cgccgtgact acgggcgcgt tctcgatctg ggaggcggga ttgcagttcg tcctcggcgc
      541 cgccggcggg gtggccgtcg ggctcgctgt cggctggttc attatctggg cccgccgcca
      601 cctctcagaa gacccttcca tacagaacac cgtctcgctc ctgaccccct tcgtctcgta
      661 cctgctcgcg gaggagttgc cttcatccct gtggcactac gccggtctgc ctggagagtt
      721 tcacttctca ggcgtcctcg ccgtggtcac gacgggcctg tactgggggc gcaaggcccc
      781 gaccgtgatc tcctccggca cacgcctcca ggggtatgcc gtgtgggagc tcgtgacctt
      841 cctgctgaac ggtctgatct tcgtcctgat cgggctccag ctcagaaacg tcgtggaaga
      901 actcggcgag tacagcgcgg gcgagctgat tctctacgcg gtactcgtta gcctgaccgt
      961 aatcctcgtc cgcatcctct gggtctttcc ggcgacatac ctcccgcgcc gggtcagccg
     1021 caggatcagg gagcgagatc ccgcccctcc ctggcaggcc gtgacggtca tagggtggac
     1081 ggggatgcgg ggtgtgatct cgctggcggc ggcccttgcg ctgccgctgc agacggcgtc
     1141 cggcgcggaa ttcgtatggc gcgacctcat catcttcctc accttctgcg tgatactggc
     1201 gaccctggtc ctccagggac tctcgctgcc ggccctgatc cgggccctcg gcctggagga
     1261 cgaccgcatc ggggataggg aggagacgca cggccgcatt ggggtcgccg aggccgccct
     1321 ggagcgcctt gaggagctcg tcgaagagga atgggtgcgc gaggacacgg ccgagcgcac
     1381 ccgcgccctc tacaactacc ggcgcaaccg cttcgccacg cgtttcgaag gcgacaccga
     1441 cggcgtggag gagcgcagcg gcgcgtacca gcgcctgatg gccgagctgc tcggagccca
     1501 gcgcaagagg ctcgtcagga tgcgcgacga gggcttgatc ggagacgagg ccatgcaccg
     1561 catcgagcgc gacctcgacc tcgaagagtc gcgcctggag atctgacccg aggaggcacg
     1621 ttatggtggc caacataacc cgcgaagagc tgaaggccaa gatggaccgc ggggatgatt
     1681 tcgtgctcgt cgaggcgcta tctcatgccc actacgccag cagtcatctt ccaggtgcga
     1741 tcaacttccc ctacgagttc gtcgacgagg cggagaagat actgccggac aaggacaccg
     1801 agatcgtggt ctactgcatg aaccgggact gctcggcttc tcgggaggag gcccgagagc
     1861 tcgatgagat gggctacgcg agggtgctcc actacgcgga cggtaagcaa gactggatca
     1921 gggccggcct gcccgtggag cggtcgggga ggtagcgcac gtgtctgaac tcctcgcagg
     1981 cttcgccctc gtcgccgttg tcttgatagt cgccgggttg acatcggggt tcgtggaacg
     2041 cgcgccgctc tcgttccctg tgatcttcct ttgtctcggc ttcttgctcg gcggtctggg
     2101 gatgcttgac ttccaccctg agagcgctgt gctcgagggc gttgccacgc tcacgctgtc
     2161 gctggtgctc tgcctggaag cggtgaggat cgggaacatc ggcatgggcg gtgtggggtt
     2221 cgtgcccatc ctttctctgg gacctgggac ggtggttatc gtggcggtca tcgccgccgg
     2281 gtcgtacttc ctgctcgatg tctcggctat cgaggcgctg cttcttggta ccatcctggc
     2341 ctcaaccgac cccgtcgtgc tcagggacgt tgtccgcgaa aaacgcatcc ccgcctccgt
     2401 gcgccaggcc ctcaacatcg aggcgggcac gaacgacatc gtcgtcttgc cgacactgct
     2461 gatcctgata gcgctggcaa acgcggcggc atcaggtgcg gcagggtggg cacggctggt
     2521 gggagaagta ctgatcctcg ggcccgtggt cggcttcgcc atcggcgcgg gcgcggcgtg
     2581 gctgatgagc catgccgacc gccgatacgc gataaacgag gtttaccaat ccctgtacgg
     2641 cgttggtata gtgcttctct cctacgcgtc ggcgaccgcc cttggcgggg atggcttcct
     2701 ggccgcgttt gccgcagggt tcgcgatcgc cgtgttgaac ttcgacctgt gccagtgctt
     2761 tcttgactac ggcgagacga cgagcgagat ggcgatgctg ctctcgttca tcctgttcgg
     2821 ggtcgtgctc tccgacctct tctctgaagt ccccctcatc cccacgctcg ccctcgcggc
     2881 catcgtgatc ttcgtcgcac gcccactggc catagggatc gtgctgcgca aggcggccgt
     2941 gagcaacgcg gcgcgggcgt tcatagggtg gttcggaccg cgcggcct
//
LOCUS       DATAIR010000468         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444643, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000468 DATAIR010000000
VERSION     DATAIR010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444643"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VHM69_11335"
     CDS             complement(<1..512)
                     /locus_tag="VHM69_11335"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX2741030.1"
                     /translation="MKLDPKIELRHLYNPSAREVSVVDVPQMYFLMIDGCGDPNTSDD
                     YAEVLEALYAVSYAIKFRVKRTEGGVDDAVMPLEGLWWTDDMREFSVEDKRSWKWTAM
                     IMQPEEYFTEGLVDEAKTEVKRKKGLPAIPRMRFEAFGEGLSAQIMHRGPFSEEGPVI
                     ERIHRGIEERG"
     gene            complement(512..2212)
                     /locus_tag="VHM69_11340"
     CDS             complement(512..2212)
                     /locus_tag="VHM69_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX2741031.1"
                     /translation="MKRTPTSFWMVRLGLVVALAIIALAAALPVIPPNPAPASAPEST
                     FSAQRAMGDLRVVAREPHPIGSAEQERVRDYIISQARTLGFSTEVQRAAVGSGRTAEN
                     VIVRIPGTENSPRDVLITAHYDSAPSSPGAGDAGASVVAMLETMRVVEASKPLKNDLV
                     FLFTDGEELGGPGAEVFVNRHPAAEDLGVAFVFDSEPDSGPTDMRTTSPGDAWLVRQL
                     VEASPPVFANSATNTSDRTRLGNDFAAFPPAGIISAEFLLEGSVVRYHSPKDNVAAID
                     PRVVQDHGDTMLALARYFGNVDLDAARSSSEDLVFFTAPVLGLVAYPVWLASVLAVMA
                     AILFIAVIVAARQRGRLSLARLTWGALAFLTMLLSGAALAWAVWELLLSMHPESASTL
                     HFPDFEGSAMAVAVIFAVAVVAFVAVTHLLSRWIGAVELAAGALVWLAVLALALTLFE
                     SLFSAVALWPLLGGVVALSVVAFLGRGWAAGVLLALASVPGLVLVVPLLVLETLKVED
                     GAVVGVASLLLLLGSLLPQILLVTGRLVPEAEDLSEQGETYQRRITRRSVDAAERRPL
                     "
     gene            2793..3944
                     /locus_tag="VHM69_11345"
     CDS             2793..3944
                     /locus_tag="VHM69_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2741032.1"
                     /translation="MNYTSRRDLTKVLEERFGNRCKPHGIHAGPLATVIPADAEEVGF
                     LAESVRRYSGRLSPAGAGTDPDLPEPTGDTVLVRTEMMRKIRFPDGGRVEVEPGVLWV
                     DLEDELRFSHQALTVYPTSAPRATVGGWVARDGMGVGSFEFGWLRENVISVEVVLPDG
                     RLRTLEGDDLGLAVGAMGRTGVIVRATLKLRETEIDRPFAISFARQETLQRAVDSLLE
                     RRPPLWHLGIVNPTMARAMRSREGYMIFGAYPASGAAAAEGELARVSGENRGAALDAA
                     EAYRMWGGRFFPVAPARPTPAPGRVLVPASRVGAVLDHLMSGPAELAVLGSVARDGSA
                     LLLAFANYGEEDRPRSLSAGDESALVGIARSVGGDLYHAVLMRRAELAR"
     gene            complement(4144..4704)
                     /locus_tag="VHM69_11350"
     CDS             complement(4144..4704)
                     /locus_tag="VHM69_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741033.1"
                     /translation="MIILALVIVLLVVGIVVFGVSAKGGRLKMLKVLYALAIALLVVA
                     FVGFGISAFYPAPEYPEPPAEVQSAYEESDPTPKQEELIREQAQREKAYEAQFSDYNQ
                     VVSSISIGVAVLLLVGSILWLSSLDVIGDGVTLGAVFTLFYGLIRAFMTESEVFRFIA
                     VGVGLVVVITLVYLRFVRSAGKAEPA"
     gene            complement(4893..5189)
                     /locus_tag="VHM69_11355"
     CDS             complement(4893..5189)
                     /locus_tag="VHM69_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741034.1"
                     /translation="MRIWRYELGTWVEEDVPLSERHPFLPEGYDHSPFMTLGDTSPRA
                     KGMSVAAHEVSEGHGYLVVVFAGEETMDPIVVSDLPSLLDLLGKLAPIVSASPD"
     gene            5313..5849
                     /locus_tag="VHM69_11360"
     CDS             5313..5849
                     /locus_tag="VHM69_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741035.1"
                     /translation="MATERSPHHTGDTGQHKRLVVGRFSFTCCEDSTILFTELLNDHL
                     VEWKRLIEFRHMKALKTNNSLSGLDVAFIEGAISSESQAREVSQIRDNAKYVVAIGAC
                     ACTGQPSTSRNQFASEQIDERIQWYLSHFDYGKDVKRLNEVIHVDDMVRGCPMKVPSF
                     MQTLDKYLELFRIGQHAR"
     gene            5839..>7107
                     /locus_tag="VHM69_11365"
     CDS             5839..>7107
                     /locus_tag="VHM69_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX2741036.1"
                     /translation="MPGRNIVIDAITKIEGNAGLKVFVEDDVVKDLQFIVADYRRFFT
                     TAVKGRRMIAVPSFLSRICGTCSVAHLFASLMAVESAQGIAVTEQTKALRRLAYDGLM
                     IRDHALHLYFFVLPDVLGVDSILDISDDPDDPGHTLLHDSFDIKRLGTDISNTVIGAA
                     IHAPWPTVGGFLKHPEPAKFPELIARLEAIRPQVLRGIETFFGWDASLVRNTDYLGLR
                     NDSRFDFLEGDVVNSDGKRISRSEFDNYLQYVQIPHSHAEGYRFSDTGEDYLVGALAR
                     INLNRDLLHPRTEDAVASSLSAFPSNNIYHNNLAQAIEILQCVDDALDILRSIEITDE
                     KPVRVPAKAGTGVGLIEAPRGLLYHMANVNEEGLIEDYDVIVPTAQNQINIENDLRDY
                     FNENLDKDEEALRIDAESIVRAYDPCMSCAT"
BASE COUNT         1494 a         2147 c         2142 g         1324 t
ORIGIN      
        1 cctcgctcct ctatgccacg gtggatcctc tctattaccg gaccctcctc cgagaagggc
       61 ccgcggtgca tgatctgggc cgagaggccc tccccgaaag cctcgaaccg catcctcggg
      121 atggcgggca agcccttctt ccttttgacc tcggtcttcg cctcatcgac aagcccctcg
      181 gtgaagtact cctcgggctg catgatcatc gccgtccact tccatgagcg cttgtcctcg
      241 acgctgaact ccctcatgtc atcggtccac cacagcccct ccaggggcat taccgcgtcg
      301 tcgacgccgc cctccgttct cttgaccctg aacttgatgg cgtatgagac ggcatagagt
      361 gcctccagga cttcggcgta atcatcggag gtgttggggt ctccgcagcc gtcgatcata
      421 aggaagtaca tctgcgggac atcaaccaca gagacttccc tggccgaagg gttgtacaga
      481 tgcctcagtt ctatcttcgg gtctaacttc atcacagcgg ccttctctca gccgcgtcaa
      541 cactccggcg agttatccga cgttgatacg tttcaccttg ctcgctcagg tcttccgctt
      601 caggcacgag tcgtcccgtg accagcaaga tctgcgggag caggctgcca agaagtagca
      661 ggagggaggc cacacccact accgcgccgt cctcgacctt tagcgtctcg agcacgagca
      721 gcgggacaac gaggacgagg ccaggcacgg aagcaagcgc aaggagcaca cctgcggccc
      781 acccccgccc caggaaggca acgacggaca gcgccacgac accacccaag agtggccaga
      841 gtgcgaccgc gctgaatagt gactcgaaca gagtgagcgc gagagcaagc accgccaacc
      901 acacaagcgc accggcggca agctccacgg cgccgatcca acgagagagc aggtgcgtaa
      961 cggccacgaa cgctactacc gcaacggcga agatcaccgc caccgccatt gcactgccct
     1021 cgaagtcggg gaaatggagt gtcgaagcag attcagggtg catagacagc aataactccc
     1081 acactgccca ggcaagcgcg gcgccggaca agagcatggt gaggaaggcg agcgcacccc
     1141 atgtgaggcg ggcgagggat aggcgtccgc gctgccgtgc ggccacgatc accgcgatga
     1201 agaggatagc cgccatgact gccagcacac tggcgagcca tacggggtaa gccacaagac
     1261 cgagaacggg agcggtgaag aacaccaggt cttcactgga gcttcgcgct gcatcgagat
     1321 ccacgttgcc gaagtagcgc gccagggcga gcatggtatc tccgtgatcc tgcacaactc
     1381 tcgggtctat ggccgccacg ttgtccttgg gggagtggta gcgtacgaca ctcccctcca
     1441 acaggaactc cgcgctgata atcccagcgg gagggaaggc cgcgaagtcg ttgccaaggc
     1501 gggtgcgatc tgaggtgttg gtggcggaat tggcaaacac cggaggcgat gcctcgacga
     1561 gctgccgaac cagccaggcg tctcccgggg acgtagtgcg catatcagtc gggccgctgt
     1621 cgggctcact atcgaagacg aacgccacgc cgaggtcttc cgcggccggg tgcctgttga
     1681 cgaacacctc tgcgccgggg ccacctagct cctccccatc ggtgaacagg aagacgaggt
     1741 catttttcag cggcttgctc gcctccacaa cccgcatcgt ctcgagcatt gcgacgaccg
     1801 acgccccggc atcccccgct ccaggagagg agggggcaga gtcgtagtgg gcggtgatta
     1861 gcacgtctcg cggtgagttc tctgtgcctg gtatgcgcac tatgacgttc tcagcagtgc
     1921 gcccagaccc taccgcagct cgctgcactt cggtggaaaa ccccagcgtc cgggcctgcg
     1981 agatgatgta gtcgcgcacc cgctcctgtt ctgcagaacc tatgggatgg ggctcacggg
     2041 ccacgaccct gaggtctccc atcgcccgct gtgctgagaa agtgctctct ggggcgcttg
     2101 ctggcgccgg gttgggcggg atgacgggga gcgctgccgc cagcgcgatg atcgccagcg
     2161 ccacgaccag tcccaggcgt accatccaaa aagatgtagg cgtgcgtttc atcgtctcac
     2221 tcctttgcct tcgacttcgt ccacggtaaa aggtatgggc gtctgtgcga ccgtagcctc
     2281 tgcgttgcgt ccctagccta actctggagt gagaagttgc cttgacacga attgatcaag
     2341 tgtgtggtca aagtgtgtca tcggcttgcg gggctcagcg ccgacgggct ccggcgctat
     2401 actgcaccct ttctctgaag gccaaagtag gccaatcctg gccagacata tgatccaaac
     2461 tgatcatggc aagcctcgct ttttccgcta gattcggaat tatagaaggc gagagcgacg
     2521 aggaggaggt gtgaagggaa gtgagcaagc tgaaggtgaa catcacacca cctgcccgaa
     2581 ctgcggcgct gagcgctcag atctcatgcg gcagacacct gcgcgatgat atcctccatc
     2641 gctggcatcg agactgcgag gagtggcaga ggttcaagga gggcgaggag agtacgagga
     2701 atggctagag cagagaatcc tggacctggt gtgatgagca gcttgaggcg gactagcctc
     2761 tcctgtcaga ggctagccgc tgaggtaagg acatgaacta cacgtcgcgc agagatctga
     2821 cgaaggtgct ggaagagcgc ttcgggaacc gctgcaagcc gcacgggata cacgcgggcc
     2881 cgcttgccac cgttattccg gcagacgccg aggaggtcgg gttcctggct gagagcgtac
     2941 gcaggtactc agggaggctg agcccggcgg gcgcgggcac tgaccccgac ctccccgagc
     3001 ccacgggaga taccgtgctg gtccggaccg agatgatgcg caagatccgc ttccctgatg
     3061 gcggccgcgt cgaagtggag ccaggagtgc tctgggttga cctcgaggac gagctccgct
     3121 tttcacacca ggcgctcacc gtctacccga cgagcgcccc ccgtgcgacg gtaggaggtt
     3181 gggtcgcccg cgacggcatg ggggtcggct ccttcgaatt cggctggctg cgggagaacg
     3241 tcatctccgt cgaggtagta ctgcccgacg gccggctgcg aaccctcgaa ggcgacgacc
     3301 tcggtctcgc cgtgggggcc atggggagga cgggcgtgat cgtgcgtgcc accctgaagc
     3361 tgagggagac tgaaatagat cggcccttcg ccatctcctt cgcgcgacag gaaacgctac
     3421 agagagccgt agactccctg ctcgagagaa ggcctcccct ctggcatctt ggcatcgtga
     3481 acccgacgat ggcacgcgcg atgagatctc gggaagggta catgatcttc ggcgcttacc
     3541 ccgcgagcgg ggccgctgcg gcagaaggcg agcttgcgcg ggtgtcgggg gagaatcgtg
     3601 gcgcagcgct cgatgccgcc gaagcataca ggatgtgggg cgggaggttc ttccccgtcg
     3661 cccccgccag gcccacaccg gctcctggca gggtgctagt gccagcttcg cgcgtcggag
     3721 cggtcctgga ccacctgatg agcggtcccg ccgagcttgc cgtcctcggg tccgtggccc
     3781 gcgacggctc cgcacttctg ctagctttcg cgaactacgg ggaagaagac cgtcctcgta
     3841 gtctctccgc cggtgacgag tccgcgctgg tggggattgc gcgtagcgtc ggcggggacc
     3901 tctaccacgc ggtcctgatg cgacgcgcgg aacttgctcg ataatcctga ccatctgatc
     3961 ggcccagctt gcttttttgt gaggatctct tgcctctacg gtagacccgc gagccgcatc
     4021 tacgacggga ttccacaggg aaggaaggat gatgaccgac cagtccttcg acctcacggt
     4081 actcagggct cgcgtactgc gtagaaacac atgcgcgtga ggtgcgcacc aggctctcta
     4141 gcttcaggca ggttcagcct tcccagcaga ccgcacaaac ctcaagtaaa caagcgtgat
     4201 caccaccaca agtcccaccc ctacagcgat gaacctgaac acctcgctct ccgtcatgaa
     4261 cgccctgatc aaaccgtaga agagggtgaa caccgcccca agggtcacac catcgcctat
     4321 aacatccagg ctgcttagcc acaggatgct gccaacgagt agcagcaccg caacccctat
     4381 ggagatggat gagactacct ggttgtagtc tgagaactgc gcctcgtaag ctttttcgcg
     4441 ttgggcttgt tcgcgtatga gttcctcttg tttcggggta gggtccgact cctcgtacgc
     4501 ggactgcact tccgcgggag gctctgggta ttcaggagca gggtagaagg cggagatccc
     4561 gaagccgacg aaggcgacaa cgaggagggc tatggccaga gcatagagta ccttgagcat
     4621 cttgagtcgt cctcctttgg cagagacccc gaatactacg atgccgacaa caaggagcac
     4681 tatgaccagc gccagtatga tcatctcccg tgcacctcct tcgagcactt ctatgccaca
     4741 ctaccgcctg aatagccgca tgtcgtgaca cgtattgatc aagtcactgg ttacatcatg
     4801 ccattcgatg tgtgaactac cgggactgct tggatctcac catcgggagt cgagcgatgt
     4861 cgtcgtgggg ccagctgtcc atggcggcgt tctcaatctg gagaagcgct gacaatcggg
     4921 gcgagcttgc caagcaggtc gagcaggctc gggaggtcgg agacaactat aggatccatc
     4981 gtctcctcgc ccgcaaagac cacgacgagg taaccatgtc cctccgagac ctcgtgggcg
     5041 gctacgctca tccctttggc acggggactg gtatccccga gcgtcatgaa gggcgaatga
     5101 tcatagcctt cgggcaagaa cggatgtctc tcggagaggg gcacgtcctc ctcgacccag
     5161 gttcctagct cgtagcgcca tatacgcatc ctcgttccat tctaatgact actcgccaac
     5221 gttcgccacc ctctttccaa tggagaagct gaatcgagct ccaggcgttg cctattcaag
     5281 cgcacatcct gtattctcca ttatgggagc acatggctac ggagagatca ccgcaccaca
     5341 caggcgacac gggccagcac aagcggctgg tcgttggacg gttctcgttc acctgttgcg
     5401 aagacagcac gatcctgttc acggagttac tgaacgacca cctcgtcgag tggaaaaggc
     5461 tgatcgagtt caggcacatg aaggcgctca agacaaacaa ctcgctctcg ggactcgacg
     5521 tggcgttcat cgaaggggcg atctcgtccg agtcgcaggc gagagaggtt agtcagatac
     5581 gggataatgc gaagtacgtg gtggcgatag gtgcttgcgc ctgcacgggg cagccctcga
     5641 cgagccgcaa tcagtttgcc agcgagcaga tagacgagag gatccagtgg tatctgagtc
     5701 atttcgacta tggcaaagat gtcaagaggc tcaacgaggt gatacacgtc gatgacatgg
     5761 tccgcggatg ccctatgaag gtaccgtctt tcatgcagac gttggataag tacctggaac
     5821 tattcaggat aggccagcat gccaggtaga aacatagtta tagacgccat cacgaagatc
     5881 gagggcaatg ccggactgaa ggtcttcgtc gaagacgacg tggtcaagga cctgcagttt
     5941 atcgtcgctg actacaggcg gttcttcacg accgcggtta agggcaggcg gatgatcgcg
     6001 gttccatcgt ttctgtccag gatctgtggc acgtgttcgg tcgcccatct cttcgcatct
     6061 ctgatggcgg tcgagagcgc gcaaggtatt gctgtcacgg agcagacgaa ggcgctgcgc
     6121 aggctggcgt acgacgggct catgatcagg gaccacgcgc tgcacctgta cttcttcgtg
     6181 ttgcccgatg tcctcggggt ggactcgatc ctcgatatca gcgacgaccc cgacgacccg
     6241 ggccacacgc tcttgcacga ttccttcgat atcaagcggc tgggcaccga catcagcaac
     6301 accgtcatcg gggctgctat ccacgcccct tggccgaccg tggggggatt cctgaagcat
     6361 cccgagcctg cgaagttccc cgagcttatc gcacgtctgg aagccatacg accccaggtg
     6421 ctaagaggga tcgagacttt cttcgggtgg gacgcgagcc tcgtccggaa cacggattat
     6481 cttggcttgc gcaacgattc acgcttcgat ttcctcgaag gcgacgttgt gaactcggac
     6541 ggcaaacgaa tctcccggtc agagttcgac aactacctgc agtacgtgca gatcccccat
     6601 tcacatgcgg agggatacag gttctccgat actggcgaag actacctcgt cggcgcgctc
     6661 gcgcgaatca atttgaatcg ggatcttctt catcctcgga cagaggatgc cgtggcatcc
     6721 tccctttcgg cgttcccttc gaacaacatc tatcacaaca acctggcgca ggcgatcgaa
     6781 atactgcagt gcgtcgacga cgccttggat atcttgagat ccatagagat cacggatgag
     6841 aaacccgttc gagtccccgc aaaggccggt acaggcgtcg gcctcataga ggcgccgcgg
     6901 gggctccttt accacatggc gaatgtcaac gaagaaggcc ttatcgaaga ctacgacgta
     6961 atagttccga cggcccagaa tcagataaac atcgagaacg acctgaggga ttacttcaac
     7021 gagaacctcg acaaagacga agaggcgttg cgtatagacg ccgagagcat cgtacgcgcc
     7081 tacgacccct gcatgtcgtg cgcgacc
//
LOCUS       DATAIR010000469         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_122526, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000469 DATAIR010000000
VERSION     DATAIR010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122526"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VHM69_11370"
     CDS             complement(<1..977)
                     /locus_tag="VHM69_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX2741037.1"
                     /translation="MQQRVNPGHKSLADYRSIIRRELYDEVVELGERLKGARVLHVSA
                     TSFGGGVAEILYTLVPLMRDAGLDTEWDIMFGSEPFFNVTKSFHNALQGADYDLSIED
                     RAIYEEYNRISAEALQKAGEEWDIMFVHDPQPALVKHFSDGLSPQTKWIWRCHIDTST
                     PNRQVLDYLLPHITDYDAQIFTMEDYTPPDVHLPGLTIIPPAIDPLSPKNMALSADDA
                     RYIVSQFGVDAARPFMVQVSRFDPWKDPLGVIDAYRLVKEEIPEIQLVLIGSMAHDDP
                     EGWDYWYKTVNYADGDRDIFLFSNLTNVGAIEVNAFQSIADVVIQKSIR"
     gene            complement(1112..1558)
                     /locus_tag="VHM69_11375"
     CDS             complement(1112..1558)
                     /locus_tag="VHM69_11375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2741038.1"
                     /translation="MERETAKTKEDGIGFSDPISLTLPSSPEYVLLARLVMGQAGRLA
                     GLEPEEVYDLKLAVTEAVTNVIRHASVESYEIEYRVLPLSVEVSVTDDGGGFSVADLT
                     GEPDEHGGFGLTVIRNLVDELVLDSTKGGGTRLKMVRRAGTPAEEP"
     gene            complement(1773..2729)
                     /locus_tag="VHM69_11380"
     CDS             complement(1773..2729)
                     /locus_tag="VHM69_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX2741039.1"
                     /translation="MGLALLQAVGIGFAFIALVVVAIFVASVLISAIKVVKEYERGVI
                     FRLGRVQGGPKGPGLFLLFPLVDKMVKIDLRTVTMDVPPQDVITRDNVPARVNAVIYF
                     RVVDPNRSVLEVENHVLATSQISQTTLRSVLGQKDLDDLLTNREAINDELQRIIDEQT
                     DPWGIKVSVVEVKDVEIPQQMQRAMARQAESERERRAKIIAAEGEYQASERLRQAADR
                     LESPTALQLRLFQTMGEIAVNQNSTIILPIPLDLFRPFLESQDGSYGEGVRAARRDEE
                     KEAERLYEEAVGDVSPEVSASEEAPDGGLPGESEPRVGRELP"
     gene            complement(2854..>4224)
                     /locus_tag="VHM69_11385"
     CDS             complement(2854..>4224)
                     /locus_tag="VHM69_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX2741040.1"
                     /translation="ERAEFIEVREAKNELASRKDELEAQREQRVKAVERAIAQQERAI
                     KAEAQAEAYLNSLNAELRAEIQAAQDRQAEQARAAAAAMAERETTPEPAPEPKPEPIP
                     EVQVSEVKQVPVPQPDLESQQAAAEREAAARAAAARAQRLAEERAAERQAAREAAEQA
                     AAEQRAAEKRAAQLAAEKQARQEARAEARRQAELAAQRAAERQAARAEAERQAELAAR
                     RAAIEQAAAEQAAAERAAARRAAERRAERLAEARAAARREARIAERRAEARAAERLAE
                     RRAEARAEARKEERLAAASASASASASASASASASASASASASASGSRADRRGGGGVS
                     GSGEDVLGVADDHLGTPYVFSPPGPCAAFEAEDCSCFTMLVFQEFGIALPDSPGGQMG
                     YGTPVHGAPQAGDLLFWSEDGSGSITHVGIAMGDGTTIHAQIHNEVTITSINAIPGYV
                     GARRLL"
BASE COUNT          676 a         1396 c         1282 g          870 t
ORIGIN      
        1 tcgcggatgg acttctggat cactacgtct gctatggact ggaaggcgtt gacctcgatg
       61 gctcccacgt tcgtgaggtt tgagaacagg aagatgtcgc gatccccatc ggcgtagttg
      121 accgtcttgt accagtagtc ccaaccctcc gggtcgtcgt gcgccataga gccgatcagg
      181 acgagctgga tctcgggtat ctcctccttg accagcctgt aggcgtctat tacgccaaga
      241 gggtccttcc agggatcaaa gcgcgagacc tgtaccataa agggcctcgc cgcgtccacg
      301 ccgaactggc tcacgatgta gcgggcatcg tcggcgctca gggccatgtt cttgggcgag
      361 agcgggtcta tggcgggtgg gatgatggtg agcccgggca ggtgcacgtc gggcggggtg
      421 taatcctcca tggtaaagat ctgggcgtcg tagtccgtta tgtgcggcag gaggtagtcg
      481 agtacctgcc ggttcggggt cgaggtgtct atgtggcaac gccagatcca cttggtctgc
      541 ggcgagagac catctgagaa gtgcttgacc agggccggct gcgggtcgtg gacgaacatg
      601 atgtcccact cctcgcccgc cttctggagc gcctcggccg atatacggtt gtactcctcg
      661 tagatcgccc ggtcttcgat gctcagatcg taatcggcac cttgcagggc gttgtggaag
      721 ctcttggtga cgttgaaaaa cggctccgac ccgaacatga tgtcccactc ggtgtcgagg
      781 ccggcgtcgc gcataagggg cactagggtg tacaggatct ccgccacccc gccgccgaag
      841 ctcgtcgcgc tgacgtgcag tacccgcgcc cccttgaggc gctctccgag ttccacgacc
      901 tcgtcgtaga gctcccgccg gattatgctg cgataatcgg ctagtgattt gtgaccggga
      961 tttacacgct gctgcatagc ctgcctactg gtcccccctc atcgcattct gaaagtacag
     1021 tttcacctac acggttaact gtatattaac gtcgcccgaa gattcaactc gatagtgccc
     1081 taaacaccgg aagttcatgc cttttctagc tttatggttc ttccgcgggt gtaccggcgc
     1141 gccggaccat cttcaaacgc gtccctccac ccttcgtcga gtcaagaacc agctcatcca
     1201 cgaggttacg gatcacggtg aggccgaacc cgccgtgttc gtcaggctcg ccggttagat
     1261 ccgcgacgct gaagcccccg ccgtcgtccg tgacgctcac ctcgacgctc aaaggcaaga
     1321 ccctgtactc gatttcgtag ctctccacag aggcgtgccg tatcacgttg gtcaccgcct
     1381 cggtaacggc cagcttgagg tcgtagacct cctccggttc gagaccggcc agacgcccgg
     1441 cctggcccat caccaggcga gccagcagca cgtactcggg gctgctcgga agagtcagag
     1501 agatcgggtc cgaaaacccg ataccatcct cctttgtctt ggctgtttca cgctccatcg
     1561 gacctctcgg tcgtctggct cccctcacgc aaactgatta gctagcttgc cggaggcgca
     1621 actttgcaac ttcgcaaatc tacttaccca gatgtatacg ctgctaaccc gctgctcgga
     1681 ccgacgggca agtgacactc gtcatcctgg gccggatagg gactatgccg gctcatggca
     1741 cgactggagc tctcctgtgg tgagggctcc ctttagggaa gttctcttcc tactcgcggc
     1801 tcactctcgc cggggagccc tccgtcagga gcctcttcgc tcgccgatac ctcgggagac
     1861 acgtctccga cggcctcctc gtacagccgc tcggcctctt tctcctcatc tcgacgcgcc
     1921 gccctgaccc cctctccgta ggatccgtcc tgggattcca ggaatggcct gaagaggtca
     1981 agaggtatag gaagaataat ggtggaattc tggttgacgg cgatctcccc catcgtctgg
     2041 aagagcctca gctgcaacgc cgtcgggctc tcaagcctgt ctgcggcctg cctgagccgc
     2101 tcggaggcct ggtattcgcc ctccgcagcg atgatcttcg cccgccgctc gcgctcgctc
     2161 tcggcttgcc tggccatcgc ccgctgcatc tgctgcggga tctcgacgtc cttgacctcg
     2221 acgacgctga ccttgatacc ccaggggtcg gtttgctcgt ctatgatcct ctggagctcg
     2281 tcgtttatgg cctcccgatt ggtcaacagg tcgtcgaggt ccttctgccc gaggacgctc
     2341 cttagagtag tctgggagat ctggctggtt gcgaggacgt ggttctcgac ttcgagaacg
     2401 ctcctgttgg gatcgacgac gcggaagtag atgacggcgt tcacgcgagc aggcacgttg
     2461 tcacgagtta tgacgtcctg gggcggcacg tccatcgtga cggttctgag gtctatcttg
     2521 accatcttgt ccacgagtgg gaacaacagg aagagacccg gtcccttagg acctccctgg
     2581 acgcggccaa ggcggaagat cacaccccgc tcgtactcct tgacgacctt gatggcgctg
     2641 atcaagacgg aagctacgaa gatggcgacc accacgagag cgatgaacgc gaacccgatt
     2701 ccgacggcct gcaagagcgc gagccccata agatcctgct cctctccggt tgcgacgaac
     2761 ctacaccgat tctaccacca ggggccgtgc cccagagaaa agcgaaggag gcccgtatcg
     2821 gacctccttc gttaccttat tggaggagct tggctagagg agcctcctcg ctcccacgta
     2881 gccgggaatg gcgtttatgg aggttatggt tacctcgtta tggatctggg cgtgtatggt
     2941 cgtaccatcg cccatggcta ttccgacgtg ggtgatggac ccgctgccgt cctcgctcca
     3001 gaagagaagg tctcccgcct ggggagcccc gtggaccggg gtaccatagc ccatctgccc
     3061 gccaggagaa tccggcaggg cgatgccgaa ctcctggaag accagcatcg tgaagcatga
     3121 gcagtcctcg gcctcgaatg ccgcgcaggg tcccggaggg gagaatacgt atggggttcc
     3181 caggtgatca tccgccacgc cgagaacatc ctcgcccgag ccgctaacac caccgccgcc
     3241 acgacggtca gcacgcgatc cagaagcgga ggccgaagca ctggctgaag cgcttgctga
     3301 agcgcttgct gaagcgcttg ctgaagcact ggcagatgcg ctggccgctg cgagtcgctc
     3361 ttccttcctg gcctcggccc tggcttcggc gcgcctctcg gcgagccgtt ctgcagccct
     3421 ggcctcggca cgcctctcgg cgatccttgc ttcccgcctg gccgccgctc tggcctcggc
     3481 gagcctctcg gcacgacgct cggcggcccg tctggcggcg gcgcgctctg cggcggcctg
     3541 ctctgcggcg gcctgctcga tcgccgcgcg ccgggctgcg agctcggcct gacgctcggc
     3601 ttccgctctc gctgcctgac gctcggccgc acgctgggca gcgagctcgg cctgcctccg
     3661 ggcctccgct ctggcctcct gcctggcctg cttctcggcg gcgagctggg cggcgcgctt
     3721 ctctgcggca cgctgctccg cggcggcctg ctctgctgct tccctcgcag cctgcctctc
     3781 ggcggcgcgc tcctcagcga gacgctgcgc cctcgcggcg gcggcccttg cggcggcctc
     3841 gcgctctgcc gcggcctgct gggactccag gtcgggctgc ggcacgggaa cctgcttgac
     3901 ctctgaaacc tgtacctcgg gaatgggctc gggctttggc tccggagccg gctcaggtgt
     3961 cgtttctctc tcggccatcg ccgcagcggc ggcgcgagcc tgctcggcct gccggtcctg
     4021 ggcggcctgg atctcagccc tgagctcggc gttgagggag ttcaggtaag cctcggcctg
     4081 cgcctcggcc ttgatggcac gctcttgctg ggcgatggcc ctctcgacag ctttgaccct
     4141 ctgctcgcgc tgggcctcga gctcgtcctt gcgcgacgcc agctcgttct tggcctcgcg
     4201 aacctcaatg aactcggccc tctc
//
LOCUS       DATAIR010000470         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444779, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000470 DATAIR010000000
VERSION     DATAIR010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444779"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="whiA"
                     /locus_tag="VHM69_11390"
     CDS             <1..426
                     /gene="whiA"
                     /locus_tag="VHM69_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX2741041.1"
                     /translation="EFIRRCAYFSRAPLKIVRRRGRWVAYTKSADGVTTVLAQMGLND
                     VVLEYEARAVLGEAKANANRVTNFDAANAGRTAKAAARQQRAFEALDPEHLPPALKEM
                     LELRLEHPDASLADLARLGGLSKSAANHRLRRLVGMAAD"
     gene            628..1629
                     /gene="gap"
                     /locus_tag="VHM69_11395"
     CDS             628..1629
                     /gene="gap"
                     /locus_tag="VHM69_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2741042.1"
                     /translation="MPVRVGVNGFGRIGMLFTKAAMVRDDIEVVAANDLMPFDSLANL
                     FKHDSTHGIWHEDVSFDGNVLRVGDHEIKTFAERDPAQIPWGDVGADIVVESTGVFTS
                     RDGAAAHIEGGAKKVIISAPGKGVDATFVFKVNHEDYDPENHEVVSNASCTTNCIIPL
                     VKVLQDNFGIESGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTAGE
                     IYPDLQGKVDGMSMRVPVSDGSITDFVARISQEASADDVNAAFKAAADGDLSGILEYT
                     EEPLVSSDIIGNPASCVFDSQLTMSNGGTVKVLGWYDNEWGYSNRTCDLAAYMGEKL"
     gene            1808..>2752
                     /locus_tag="VHM69_11400"
     CDS             1808..>2752
                     /locus_tag="VHM69_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2741043.1"
                     /translation="MNKRSVRDLDVGDKRVLVRVDFNVPVKDGEVTDDTRIVRALPTI
                     RHLLSEGGRPILISHLGRPKGEPDPKYGMDPVAARLRELLGEPVTKLDAAVGPEVEEA
                     LDDWDGEGVVLLENSRFYPGETKNDPGLAGQLAALADLYVNDAFGAAHRAHATTVGVA
                     ERLPAAAGLLMEEEIDYLDKVLEDPDRPFVAILGGAKVSDKLGVIESLLGTADSLLVG
                     GAMGFTFFKALGHEIGDSLVEDEYLEEAKRLMDEAGDRLVLPVDVVVADAMEEGAESE
                     TVPVDGIPSGKMGLDIGPETVALFEGRISDASTIFWNGP"
BASE COUNT          553 a          838 c          909 g          452 t
ORIGIN      
        1 gagttcatca ggcgttgcgc gtacttctcc agggcgccat tgaagatcgt gcgcaggcgc
       61 ggaaggtggg tcgcgtatac caagagcgcc gacggcgtta cgaccgttct tgcccagatg
      121 ggcctcaacg acgtggtcct ggagtacgag gcaagggccg tactcggcga ggccaaggcc
      181 aacgccaacc gcgtcaccaa cttcgacgcg gcaaacgcgg gacgcaccgc gaaggccgcg
      241 gcccgccagc agagagcctt cgaagccctg gatcccgaac atttgccccc tgcgctcaag
      301 gagatgctgg agttgcggct cgagcacccc gacgcctcgc tcgcggacct ggccaggctc
      361 gggggtctct ccaagagcgc cgccaaccac cgattgagga ggctggtcgg gatggcggca
      421 gactgagcaa gggtgtggat attgaaaccc cgttcgagaa aagtagctca tgcaggtgga
      481 tttcgctttg tgagcgtgag cggcgtcgtt cgaggaagag ctgacagctg aaagcgaagc
      541 ccgaaggcgt agcgtgctgg tgtgttaatc ttgcgggatt ttgggcaaaa taacgattta
      601 gaggcaaact agagactgga gagaagagtg cccgtaagag taggcgttaa tggtttcggt
      661 cggatcggca tgctgttcac caaggcggcg atggttcgtg acgacatcga ggtcgtcgcg
      721 gcgaacgacc tgatgccttt cgacagcctg gccaacctgt tcaagcacga ctccacccac
      781 ggaatctggc acgaggacgt gtccttcgac ggaaacgtcc tgcgcgtcgg agaccacgag
      841 atcaagacct tcgccgagcg tgacccggcc cagattccat ggggcgacgt cggcgccgac
      901 atcgtcgtcg agtccacagg agtcttcacc agccgcgacg gagcggcggc ccacatcgaa
      961 ggcggggcga agaaggtgat catctcggcc cctggcaagg gcgttgatgc caccttcgtc
     1021 ttcaaggtca accacgagga ctacgacccc gagaaccacg aggtagtctc caacgcctca
     1081 tgcacgacca actgcatcat ccctctggtg aaggtgttgc aggacaactt cggcatcgag
     1141 tccggctaca tgaccaccgt ccacgcgtac accaacgacc agagcctcct cgacgcggcc
     1201 cacaaggacc cgaggcgcgc caggtcggcc cccaacaata tcgtcccgac ctcaaccggg
     1261 gcggcgcgga cggcgggtga gatctacccc gatctccagg gcaaggtcga cgggatgtcg
     1321 atgcgcgtgc ccgtctcaga cggctcaatc acggacttcg tggcccgcat ctcgcaggag
     1381 gcttccgccg acgacgtgaa cgccgcgttc aaggcggccg ccgacgggga cctctcgggc
     1441 atcctggagt acacggagga accgctggtc tcgagcgata tcatcggcaa cccggcgtcg
     1501 tgcgtcttcg actcacagct caccatgtcc aacggcggga ccgtcaaggt gctcggctgg
     1561 tacgacaacg agtggggcta ctcgaaccga acgtgcgacc tggccgccta catgggtgag
     1621 aagctgtaga aaggcatcag gctacaggta tcaggtaagt ccgccggggt gcggccggag
     1681 ttcgtgatca cgacccggcc gcatccttcg gtgagggacg tatcccgagc aacgatagtt
     1741 tggcgggcgt tggtgacttc agcctggtag aaactgaaac ctgatgccgc cgaccgaagg
     1801 gaggcaaatg aacaaacgca gcgtcagaga cctcgacgtt ggggacaaac gggtgcttgt
     1861 gcgggttgac ttcaacgtgc ctgttaagga cggcgaagtc acggatgaca cgcgcattgt
     1921 gcgcgcgttg ccgaccatca ggcacctcct ctccgagggg gggcgtccga tcttgatctc
     1981 ccacctcgga cgtccgaagg gcgagcccga cccgaagtac gggatggacc ctgtcgccgc
     2041 gcgactccgg gaactgttgg gcgagccagt cacgaagctc gacgccgcgg tcggccccga
     2101 ggtggaagag gccctcgacg attgggacgg tgagggcgtc gtgctcctgg agaactcccg
     2161 gttctatcct ggcgagacga agaacgaccc tgggctggcg ggtcagctcg cggcccttgc
     2221 cgacctctac gtgaacgacg cgttcggcgc ggcccacagg gcgcacgcaa cgaccgtcgg
     2281 ggtggcagag aggttgcctg ccgcggccgg gctcttgatg gaagaggaga tcgactacct
     2341 cgacaaggtc ctcgaagacc cggatcgccc cttcgtcgcc atcctcggcg gggcgaaggt
     2401 ttcggacaag ctcggcgtca tagagagcct gctcgggacc gcggactcgt tgctcgtagg
     2461 cggcgccatg ggcttcacgt tcttcaaggc cctcgggcat gagatcggcg actccctcgt
     2521 cgaggacgaa tacctcgagg aggcgaagcg cctcatggac gaggccggag acaggctggt
     2581 ccttcccgtg gacgtggtcg tcgcggatgc tatggaggag ggcgccgagt cagagaccgt
     2641 acctgtcgac ggcatcccgt ctggcaagat gggcctggat ataggccccg agaccgtcgc
     2701 cctattcgaa ggaaggatct ccgacgccag cacgatcttc tggaacggtc cc
//
LOCUS       DATAIR010000471         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444853, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000471 DATAIR010000000
VERSION     DATAIR010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444853"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1544)
                     /locus_tag="VHM69_11405"
     CDS             complement(366..1544)
                     /locus_tag="VHM69_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2741044.1"
                     /translation="MASRTEQQEIERVSGWQQELAALHSRIAPRFARPEVRERAGRFL
                     RGLLGPVERRNGWQLAEAMGERTPDGVQRLLNGSRWDAGEVRDDLRSYVVEHLGHPEA
                     VLVIDETGFLKKGEKSVGVARQYSGTAGKVENCQVGVFLAYAAPRGRAFIDRALYLPE
                     EEWATDGQRRAAARVPAEIGFATKGELAQRMLSGAFEAGVPAKWIIADEVYGNGGKLR
                     RWLEARKKPYVLAVARSHPLMVAFRSKRAEEVVAEAPPEAWARVQIGAGSKGPRVYDW
                     ARARLPYENAPGWARWLLVRRSVSQPQELAYYRAYGPEEATVEELAKVAGSRWSIEEG
                     FERAKGEVGLDDYEVRYFDSWHRHITLCLLAHAFLEVSRAAANDRMGNGASTPGKKGS
                     "
BASE COUNT          297 a          600 c          464 g          315 t
ORIGIN      
        1 gagttatccg cctgacccct agggagcggg aagtgctcgc gctgctgctc cgccgatact
       61 cgaatgccga aatagccgag tccctcgacg tgagcccgcg gacggtaaaa atcatgtggg
      121 caaggttttc gtgaaacttg gggtgggggg acgcaaggag ctgttcaagg gctctttcga
      181 ctagttctac aaccgcactt cactactcga gacatcgcgt ttcttccagt ttcggtaatg
      241 acagcggcgc gcatcggcct gccgtcttcg acgccatttc gaccagtgca gcctgaagcc
      301 acgctcctcg accggccgcg cgagcatgag cagtaatcgg cgtacttccg gggcggtgag
      361 ggggatcagc tccccttttt tccgggggta gacgctccat ttcccatccg atcgttcgct
      421 gcggcacggc tcacttccag aaaggcgtgc gccaaaaggc aaagggtgat gtgccgatgc
      481 cagctatcga agtacctcac ctcatagtca tcgaggccga cctctccctt cgctcgctca
      541 aacccctctt cgatcgacca tctgctgccg gccacctttg ccaactcttc caccgtggcc
      601 tcttctggac cgtaggctcg gtagtaggcg agctcttgtg gctggctcac cgagcggcgc
      661 accagaagcc accgcgccca ccctggggcg ttttcgtagg gcagacgggc cctcgcccag
      721 tcgtaaacac gcggaccctt gctgcccgcg cctatctgca cccgcgccca cgcctccggc
      781 ggcgcctcgg cgacgacctc ctcggcgcgt ttgctccgga atgccaccat gagcggatgc
      841 gaacgcgcca ccgccagcac gtagggcttc ttccgagctt ccagccaccg gcgcagcttc
      901 ccaccattac cgtagacctc atccgcgatg atccacttgg cgggcacccc tgcctcaaaa
      961 gcaccagaga gcatcctctg ggccagctcg cccttggtag cgaaccctat ctccgccggg
     1021 acacgcgccg ctgcccgccg ctgcccgtcg gtggcccact cctcttccgg caaatacagc
     1081 gcccggtcta tgaaggctcg cccgcgcggg gcggcgtagg ccaaaaacac cccgacttgg
     1141 cagttctcga ccttcccggc ggtccccgag tactggcggg cgaccccgac cgacttctct
     1201 cccttcttca agaagcccgt ctcgtcgatc accagcacgg cctcgggatg cccaagatgc
     1261 tcgaccacgt aggagcgcag gtcgtcgcgt acctctccgg catcccagcg cgatccgttc
     1321 aacagtcgct ggacgccgtc gggagttcgc tcacccatag cctcggcaag ttgccatccg
     1381 ttgcgccgct ctaccggccc gagcaagccg cgcagaaagc gtccggctcg ttctctgacc
     1441 tccggacggg cgaaacgagg ggcgatgcgc gaatgcagcg cggccaactc ttgctgccat
     1501 ccgctgaccc tctcgatctc ctgttgttcc gtacgacttg ccatcgctcg cctcccactt
     1561 cttgcccaca cgagaagcgt acacctctaa agtgcggttg tagaactagg gcctgtctga
     1621 cgaatcatga atgcagccac aacatcagcg aggcgatgac caccatcgcc cggtag
//
LOCUS       DATAIR010000472         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_444961, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000472 DATAIR010000000
VERSION     DATAIR010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444961"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VHM69_11410"
     CDS             <1..1532
                     /locus_tag="VHM69_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2741045.1"
                     /translation="VSRVLGMTLLLTSLLAALFSVILRQRRARGDGRQQLNWFLFAAV
                     PLTVLGSLDTIDSIVADFTTNFMFQPVYMLYERGLLLPNIYVLWLAVLLVPVCTYIAI
                     LRYNLYDIDVVINRTLVYGALSACVVGIYILAVVALGAIFQAQGNLAVSLSATGLVAV
                     LFQPLRSGLQRSVNRLMYGERDDPYAVISRLGRRLEAAIAPDTVLPTVVETIAQALKL
                     PYAAVLLKEGEGFTTAAAYGSPTSEPEALPLVYQREEIGRLVLSPRAPGERFSDADRI
                     LLEDLARQAEVAVHAVRLTADLQHSRERLVATREEERRRLRRDLHDGLGAQLAGLNVQ
                     AGTLRRLIPHDPDAADELVVELRDELRGAIADIRRLVYDLRPPALDDLGLVEALRQLA
                     ERYGSKDGPLHVVVEAPEDLPDLPAAVEVAIYRITQEALTNVARHARARTCVVRLAVN
                     DDVTLEITDDGVGIPAERSAGVGLSSMHERAEELGGSCVVESIREGGTRVLVRLPLAK
                     G"
     gene            1538..2185
                     /locus_tag="VHM69_11415"
     CDS             1538..2185
                     /locus_tag="VHM69_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741046.1"
                     /translation="METLRILVAEDHPLFRKGMISLLSSVPEFEVVGEAKTGEEAVVR
                     AVQLQPDVVLMDLRMPEVNGIEATKRILQQNPSVRILVVTLFEDEDSVFMALRAGARG
                     YVLKDADEEEMVLSIRAVGKGEAIFSPSVATRVLAYFAASSPGGASPQAFPTLTDRER
                     EILHLIAQGHPNPSIARQLSLSTKTVGNYVSNIFTKLQVADRAQAIIRAREAGLG"
     gene            complement(2366..>2688)
                     /locus_tag="VHM69_11420"
     CDS             complement(2366..>2688)
                     /locus_tag="VHM69_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEX2741047.1"
                     /translation="FLGHPDHDPHGDPIPSAEGTLEVDDSFPLSQAAAGQRLRIAKVR
                     DEDAAMLDYFGDRNLVPGRRLTVREVRAIDGVVIVEDEEAEVYALGEPLARSMFVRSE
                     PELR"
BASE COUNT          512 a          843 c          819 g          514 t
ORIGIN      
        1 gagtgtcgcg tgttctgggt atgacgctgc tgttgacgag ccttctcgcc gcactctttt
       61 cggtcatact ccggcagcgt cgtgcgaggg gagatggacg ccagcaactc aattggtttt
      121 tgttcgccgc tgtgccgtta actgtcctag gtagcctgga cacgatcgac agtatagtcg
      181 ccgacttcac cacgaacttc atgttccagc cggtttacat gctttatgaa cgggggcttt
      241 tgctgcccaa tatctatgta ctgtggttgg ctgtgctgct cgttccagtc tgcacctaca
      301 tcgccatcct ccgctacaac ctctacgaca tagacgtggt cataaaccgc accctcgtat
      361 acggcgcact gagcgcctgc gtagttggga tatatatcct tgcggtggta gccttgggtg
      421 ccatattcca ggcgcagggc aacctcgccg tctcactctc ggccacgggg cttgtcgccg
      481 tcctgttcca gccgctcagg agcgggctcc agcgcagcgt caaccgcctg atgtacggcg
      541 agcgagacga cccttacgct gttatctcgc gtctggggag acgcctcgaa gcagccatag
      601 cccccgatac ggtgctgcca acggtcgtcg agactatagc ccaggctctg aagctgccct
      661 acgcggctgt cctcctaaaa gagggcgaag ggttcaccac tgctgccgcg tacggctcac
      721 ccacatcaga accggaagcc ttgccgctgg tgtaccagag agaggagata gggcgtctgg
      781 tactctctcc cagagcacct ggcgaaaggt tctcagatgc cgaccgtatc ttgctcgagg
      841 acctcgcccg acaggccgag gtagccgtgc atgcagtcag gctcaccgcc gacctgcaac
      901 attcgcgcga gcggctggtc gcaaccagag aggaggaacg ccgtcgtctg agacgtgacc
      961 tgcacgacgg tctaggcgca caacttgcgg gattgaacgt tcaggccggg actctgcgcc
     1021 gcctcatccc acatgaccct gacgccgctg atgagctggt ggtagagctg cgcgacgaac
     1081 tccgcggtgc catagccgat atccggcgcc tcgtttacga cctcaggccc ccagccctcg
     1141 acgaccttgg gctcgtggag gcactgcgcc agctcgccga gcgctacggc tccaaagacg
     1201 gacccttgca cgtagtggtt gaggcacctg aggaccttcc cgacctcccg gcagcagtcg
     1261 aggtggccat ctaccgcatc acgcaggagg cgctcaccaa cgtcgcgcgg catgcccggg
     1321 ccaggacgtg tgtcgtgcgc ctggccgtga acgatgatgt cacgcttgag atcacagacg
     1381 acggcgtggg cattcccgca gagcgcagcg ctggcgtagg actctcctcg atgcacgaac
     1441 gggcagaaga gcttggtggc agctgcgtcg tcgaatccat acgagagggc ggtacccgcg
     1501 tgctggtccg gctaccgctt gcgaaggggt gagaaggttg gaaaccttgc gtatcctcgt
     1561 tgccgaagac catcctcttt tcaggaaggg catgatctcc ctgctctcct cggtgcccga
     1621 gttcgaggtc gtcggcgagg ccaaaacggg cgaggaggcg gtagtgcgtg ccgtacaact
     1681 gcagcccgac gtggtcctga tggacctgcg gatgccggag gtaaacggca tagaggcaac
     1741 gaaaaggatc ctgcaacaga acccgagcgt gcgcatcctg gtggtaacgc tcttcgagga
     1801 tgaggactcg gtgttcatgg ccttgagagc gggggcgcgt gggtacgtcc tcaaggacgc
     1861 agacgaggag gagatggtgc tctcgatacg ggctgtgggc aagggagagg cgatcttcag
     1921 cccgagtgta gcaacccggg tactcgccta cttcgccgcc tcttccccag gaggagcctc
     1981 tccccaggcc ttcccgacgc tcaccgaccg agagcgtgag atcctgcacc tcatcgccca
     2041 gggccacccc aatccttcaa ttgccaggca actttcacta agtaccaaga ccgtaggcaa
     2101 ctacgtctca aacatcttca ccaagctgca agtcgccgac cgggcgcagg cgatcatccg
     2161 cgcccgtgaa gccggactgg ggtagggcaa ggaggggtgg caagaaacag aaggacgccc
     2221 tgagggatag tcaggtgtat ctgtcgttgc atttcttgcc acaagcgctc cgagagaagc
     2281 cgttctatgc tgaaggcgac tgccccgtgc ttggccattg cacatcgctg ccctacgaaa
     2341 tggcacagct tgcacaccgg gttaactaac ggagttctgg ctcgctccgc acgaacatcg
     2401 agcgcgccag cggctctccg agtgcataga cctcggcctc ttcgtcctcg acgatcacga
     2461 ccccgtcaat ggcccgcacc tccctgacgg tcaaccgtct tccgggtacg aggttgcggt
     2521 ccccgaagta gtcgagcatc gcggcgtcct cgtctctgac cttggcgatg cgcagccgct
     2581 gtccggcggc ggcctggctc aatgggaaag agtcgtccac ctccagagtg ccttccgcag
     2641 aggggatggg gtccccatgc gggtcgtgat ccggatgccc cagaaact
//
LOCUS       DATAIR010000473         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_29886, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000473 DATAIR010000000
VERSION     DATAIR010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29886"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHM69_11425"
     CDS             <1..307
                     /locus_tag="VHM69_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2741048.1"
                     /translation="LFFLLRPESLGTGPQDRTFDLSIEGGEMNPSEISVNQGDMVTLH
                     ISSDERMEVHVHGYDVEREVVPGQAARLHFEADLTGRFEIEDHDTEEELGVLEVRPR"
     gene            318..1826
                     /locus_tag="VHM69_11430"
     CDS             318..1826
                     /locus_tag="VHM69_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741049.1"
                     /translation="MRRTAAAALAFSFLLAEARPALAHGFGPTYDIPVPLWLYLYGAA
                     AAVLLSFVPLALFTGKEHGADTPYRYPRFDLFQIVFLRTLLTSRPLVAGLRLLSVALF
                     FLVVIAGLVGLQSGFNIAPTFVWVTWWVGLSFFTAFVGNVWPLVNPWRIIFDRAEGLA
                     RRLGYKDGLELGEPYPEALGIWPAVGLYLVFVWVENVFSGSAVPRNIALFALSYSLVT
                     LYGMAYFGRETWLRHGEVFSVFFGLLGRFAPTEVRVKSANICRDCDACGQTAGGECVN
                     CYECFARAAPAERELNLRPPAVGLARPERVPPGGVAFVIVVLAGVTFDGLLETPLWLE
                     IVRLTPVTQTLGVILLPLLFLGVYVGFVELSRVLGGGGMSLRLFASAYAFSLLPIAIA
                     YQVAHFYTYLLIQGQMIVSLISDPFGWGWDLFGTADFEPRYGIVGAGFVWYSQVALIV
                     AGHVIAVYLAHSTSLRLLRDPGRAFRSQLPMLVLMVLYTICSLWILAQPIVK"
     gene            1963..2955
                     /locus_tag="VHM69_11435"
     CDS             1963..2955
                     /locus_tag="VHM69_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741050.1"
                     /translation="MSGEDSNSHTRLEIGRTLERARRERGLSLDEVEQATKIRARYLR
                     DLENENFDVLPAVYILGSLKTYAGYLGLDGEALARELKHRQAVVESQNHEEPQAGEPQ
                     GLLASLSRLLGVENTEKDEDDSGAVPDHSPRLYMSLGVVLIFVLAIALASTLRTEDQP
                     SVSQVHETRVSKFPSMIALSGTLGDERNTDEEKEENQPGKQAKASAKVGSKGEKDKAE
                     HSEKSTHLTAQASSANASASAAVSMASSGNASAAARASAGDRQERAAAVEVDGAAEVE
                     GAAGRGVAADASAGPSARAHAGPVVQRKQAGPINAPRLRNRIASQAWSVVDDAW"
     gene            complement(3000..>4491)
                     /locus_tag="VHM69_11440"
     CDS             complement(3000..>4491)
                     /locus_tag="VHM69_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX2741051.1"
                     /translation="LLGDEQDAQYVPSTRHEGKEEERVGMQVGTFVAYLVASALFIVG
                     IRRLRAPETARSGNTLAAVGMVIALVATIFVADIDEALSGIEILAGVLVGSVIGAVAA
                     QRVQMTAMPQMVAAFNGVGGATAALVALSEFYQAEALGTHPETVVATVAVLLSVVIGS
                     VSFSGSIIAFTKLQELALTGSVTFPLQQVVNAIIALVIVGLAVDVLLGGLLLPPLVSL
                     YLILASSLLLGVLFVIPIGGADMPIVISLLNSFTGIAAAASGFVLDNYALIISGTLVG
                     ASGTILTREMSSALGRPLSKIIFAGIAASGTASKGGDDEERPVHDVSAGAVGEYLSDE
                     AQKVVIVPGYGLAVAQAQGPMYDLMETLESQGKEVLFGIHPVAGRMPGHMNVLLTEAG
                     VPYDKLYDLEDINPEFEDADAAIIVGANDVVNPAANDPEQDTPISGMPILEVDKADRV
                     IFVKRSLSPGFAGVDNPLFYDTEKTMMLFSDAKQGLQEIVEAVKNG"
BASE COUNT          796 a         1398 c         1463 g          834 t
ORIGIN      
        1 gctgttcttc cttctgcggc cagagtctct cggtacagga ccgcaggacc ggactttcga
       61 tctctcgatc gagggaggcg agatgaaccc gagtgagatc tccgtgaacc agggcgacat
      121 ggtgacgctc cacataagct ccgacgagcg gatggaggtc cacgttcacg gctacgatgt
      181 tgagcgggag gtcgtgccgg gacaggcggc cagacttcat ttcgaggcgg acctcacggg
      241 acgcttcgag atcgaagacc acgatacaga agaagagttg ggcgtgctcg aggtgcgccc
      301 gcggtaggga tgggctgatg cgtcgcaccg cggcagccgc gctcgcgttc tcattcctcc
      361 tggctgaagc caggccggcg ctggcccacg gcttcgggcc gacctatgac atccccgtgc
      421 cgctatggct ctacctctac ggagccgcgg cagccgtgct tctctccttc gtgccgctcg
      481 ctctgttcac cggcaaggaa catggcgcgg atactccata caggtaccct cgcttcgacc
      541 tgtttcagat cgtgtttctc aggacgctcc tgacttcgcg accgctcgtg gctggcctgc
      601 gcctgctctc tgtcgcgctc ttcttcctcg tggtgatcgc cggcctcgta ggtctgcaga
      661 gcggcttcaa catcgccccg acgttcgtgt gggtgacgtg gtgggtgggc ctcagcttct
      721 ttacagcttt cgtcggtaac gtgtggccgc tagtgaaccc ctggaggatc atcttcgatc
      781 gggccgaagg gctcgcgcgc cggctcgggt acaaggacgg gctggagctc ggcgagccct
      841 atccagaagc tctgggcatc tggcccgcgg tgggcctcta tctcgtgttc gtctgggtcg
      901 agaacgtctt ctccggctcg gccgtgccgc gcaatatcgc cctattcgcc ctctcgtact
      961 ccctggtcac gctctacggc atggcttact tcggcaggga gacctggctg agacacggcg
     1021 aggtgttctc tgtttttttc ggcctgctcg gcaggttcgc gccgaccgag gtaagggtga
     1081 agtcggcgaa catttgcagg gactgcgatg cctgcggcca gactgccgga ggggagtgcg
     1141 tgaactgtta cgagtgcttc gcacgcgccg cgccggcgga acgagagctg aacctccgcc
     1201 cgcccgccgt cgggctcgct cgtcctgaaa gagtgccgcc cggcggcgtg gccttcgtga
     1261 tcgtcgtgct ggccggtgtt accttcgacg gcctgctcga gaccccgttg tggctcgaga
     1321 tagtacgtct gacccccgtg acccagacgc tgggtgtgat cctgctgcca ttgctgttcc
     1381 tcggcgtcta tgtgggcttc gtggagctca gtcgcgtcct cggtggggga gggatgagtc
     1441 tgaggctgtt cgcgtccgct tacgcattct ccctcttgcc catcgcgatc gcctaccagg
     1501 tggcccactt ctacacgtac ctgctcatcc aggggcagat gatagtctcg ctcatatccg
     1561 accctttcgg gtggggctgg gacctcttcg ggaccgcaga cttcgagccc cgctacggaa
     1621 tcgtaggggc gggcttcgtc tggtactcgc aggtcgcgct cattgtggcg gggcacgtga
     1681 tcgccgtcta tcttgcccac tcgacctcgt tgcgcctcct gcgggatccc ggacgcgcct
     1741 tcaggagcca gctcccgatg ctcgtcctca tggtcctcta cacgatatgt agcctctgga
     1801 tcctggccca gccgatcgtc aagtagaact gttccctgcc agggacaaaa agactacgca
     1861 aagggtgttc agagacgctg ccgggtgtgg tacattccag gttgtcgaac gcgcgacgtt
     1921 ggtctacccg gggggagcag acacagatgg gcctgggtga aaatgagcgg cgaagacagt
     1981 aactcgcata cgcggctgga gataggacgg accctggaga gggcccgcag ggagcgaggg
     2041 ctctccctcg atgaagtcga gcaggcgacg aagatccggg ccaggtacct ccgtgacctg
     2101 gagaacgaga acttcgacgt gctgcctgcc gtgtacatcc tcgggtccct gaaaacatac
     2161 gccgggtacc tgggactgga cggagaggcg ctggcccgcg agctcaagca ccgccaggca
     2221 gtggtcgaaa gtcagaacca cgaagagccc caggctggcg agccacaggg gctactcgca
     2281 tcccttagcc gcctccttgg cgtcgaaaac accgagaagg atgaagacga ctcaggggcg
     2341 gtacccgacc acagcccccg cctgtatatg agccttgggg tggtcctgat cttcgttctg
     2401 gccatagccc tcgcgtcgac cctcaggacg gaagatcaac cctcggtctc tcaagtgcac
     2461 gaaacgaggg tctcgaagtt tccatccatg atcgccctct ccggtaccct gggcgacgaa
     2521 cgtaacaccg atgaagagaa ggaagagaat cagcccggga aacaggccaa agcgtccgcc
     2581 aaagtcggaa gcaaaggcga gaaagacaag gcggagcata gcgagaagag cacacacctg
     2641 acggcacagg cgtcttcggc caacgcgtcc gcgagcgcgg ctgttagcat ggcatcctcg
     2701 ggcaacgctt ccgcggctgc gagggcttca gccggcgaca ggcaggaacg ggcggctgcg
     2761 gtagaggtag acggcgccgc agaggttgag ggcgccgcag ggagaggagt cgccgccgat
     2821 gcttcagccg gtccatcggc ccgcgcgcac gctggacccg tcgtacagcg gaagcaagca
     2881 ggacccatca atgccccgcg gctccgcaac aggatcgcca gccaggcgtg gagcgtcgtt
     2941 gacgacgcat ggtgaccatc atttccagag gcccgcgcag gagagtcgtc tgatcggctc
     3001 tagccgttct tgacggcctc gacgatctcc tgcagcccct gcttggcgtc ggagaagagc
     3061 atcatggtct tctcagtatc gtagaagagt gggttgtcca cgcccgcgaa gcctggacta
     3121 agggaacgtt tgacgaagat gacgcggtcg gccttgtcca cttccaggat gggcatgccc
     3181 gagatggggg tgtcctgttc tgggtcgttg gccgccgggt tgacgacgtc gttggcgccg
     3241 acgatgatcg ccgcgtcagc gtcctcgaac tccgggttta tgtcttcgag gtcgtatagc
     3301 ttgtcgtagg ggacgccggc ctccgtcagc aggacgttca tgtgcccggg catgcgaccg
     3361 gccacagggt gtatgccgaa aaggacctcc ttgccctgcg attcgagcgt ctccatcagg
     3421 tcgtacatcg gtccctgggc ctgggcgacc gcgaggccgt agcctggtac tatcacgacc
     3481 ttctgggcct cgtcggagag atattcgccc accgcgcccg cgctcacgtc gtggaccgga
     3541 cgctcctcgt cgtcgcctcc cttgctggcc gtccccgagg cggcaatacc ggcgaagatg
     3601 atcttggaca gcggtcttcc cagggcgctg ctcatctcgc gcgtcaggat cgtgcccgaa
     3661 gccccgacga gcgtgccgct gatgatgagg gcgtagttgt cgagcacgaa gcccgaggcc
     3721 gccgcggcga tccctgtgaa cgagttcagt agcgagatga cgatgggcat gtcggccccg
     3781 cctattggta tgacgaatag cacgccaagc agcagcgagg aagcgagtat gaggtaaagg
     3841 gagacgagcg gggggagcag cagcccgccg agcaggacgt ccaccgcgag ccccacgatg
     3901 acgagggcta tgatggcgtt gaccacctgc tgcagcggga acgtgaccga gcctgtgagc
     3961 gcgagctcct gcagcttggt gaaagcgatg atgctgcccg agaagctgac gctcccgatg
     4021 acgacgctaa ggagcacggc gaccgtggcg acgacagtct cggggtgggt gccgagcgcc
     4081 tcggcctggt agaactcgga gagggcgacg agcgccgccg tcgcgccgcc gacgccgttg
     4141 aaggccgcga ccatctgggg catcgccgtc atctgcaccc gctgggcggc gacggcccct
     4201 ataaccgatc ctacaagcac cccggccagg atctctattc cgctcagagc ctcgtctatg
     4261 tcggcaacga agatcgtcgc aacgagcgcg atgaccatgc cgacggcggc cagggtgttc
     4321 ccggagcggg ccgtctcggg agcgcgcagt cgccttatcc cgacgatgaa gagcgcggat
     4381 gctacgaggt aggctacgaa cgtccctacc tgcatgccta ccctttcctc ttctttacct
     4441 tcgtgacggg tggacggaac atactgagca tcctgttcgt cacccagaag c
//
LOCUS       DATAIR010000474         7688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_445445, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000474 DATAIR010000000
VERSION     DATAIR010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445445"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="pyrF"
                     /locus_tag="VHM69_11445"
     CDS             <1..666
                     /gene="pyrF"
                     /locus_tag="VHM69_11445"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2741052.1"
                     /translation="LQAAFFERLGPAGMGIYAKLIEAAADLDLPVIADVKRGDIGPVA
                     AAYAEAHLGVYGAICVTVNPYMGEDAVLPFLEEARRRSVGGGVFVLVATSNPSSPAIQ
                     EATSPPLFEVAAGLVAELGTTGGDYPDAGAVVGVTRPEIGGRVRELLPDALFLSPGYG
                     AQGGEASGVRPLLDRRGGGVLVNSSRGILYAYEGSTSGYREAAGEAARRARDALCRAG
                     ARM"
     gene            complement(920..1471)
                     /locus_tag="VHM69_11450"
     CDS             complement(920..1471)
                     /locus_tag="VHM69_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2741053.1"
                     /translation="MKRLAIVVFMVVAAALLYRSFGSAETGTGSLTLPQPAPNVGDHA
                     PEFTAKSVEGRSFELSDDGVYVLTFWSTLNKYSNQAVPGFEDLAREYEHDGVSFAAVY
                     VNSAPSEDGVPYTMLLDSTGELVSKYNVKRVPRLFVVEDGTVEYTLDYYYDGYEKGIS
                     EALDETLAKKPEKGGQSALRGER"
     gene            1574..2746
                     /locus_tag="VHM69_11455"
     CDS             1574..2746
                     /locus_tag="VHM69_11455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX2741054.1"
                     /translation="MLKIADTRITKRFVACILVLLLGAQVGAGAAFAQGAREVAEPDV
                     AARAWALTDLRSGEYLAGEKSSKKLPIASTTKIMSALVVLKQGNLDEEVTVSEEAAAY
                     ATPAYSNVGLLSGDTLSVRELLMATLISSGDDAAYALAEHVGGGGGVDRFVAEMNEEA
                     KTLGLEDTHFENPVGFDASAHYSSARDLAEMARMAMRNPEFREIVSTEYATIYTPYRE
                     IPLANTNELLYSYGPATGVKTGTTPAAGETLVSSATIGDESYVCVVLDSREDRFAGSV
                     RTLRYGFAAHDRANLVVRGKRYAGVDVPYRRDKSVDLLAEDDVDGLVDASPDVERDVE
                     IVKDLPDSARAGTRLGEVDVRVDGKKVGETPLVARTGYEKAPPWQRVWYTVEGIFE"
     gene            2762..3721
                     /locus_tag="VHM69_11460"
     CDS             2762..3721
                     /locus_tag="VHM69_11460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX2741055.1"
                     /translation="MILTITLNAAVARTLVVPSLTLGHRHRAPESITLAGGKGVNVAR
                     SLRTLDVPVLAAGFAGGRNGDAIRDGLSEAAIPFDLVEIEGYSRTSTAIIDPMAGTQT
                     EINEYGPEVTQAEAKEFSRRLEHLMEYATAVVFAGSVPPNLDESFVVGLVRRARERGL
                     YTVVDSPPSVLTAALKANPSLVSPNQHEAESVVGFDFIEDGDFPLGLSRLVEFGAADA
                     CITSPEGLSYLSTENGVVSARAPKVETVSTVGSGDAYLAGLLAGLLHRNLAPVEAVRL
                     AAGCAAANAETLGAGLFEARRAEELADSVQVEVISRSLEQSRT"
     gene            3812..4123
                     /gene="trxA"
                     /locus_tag="VHM69_11465"
     CDS             3812..4123
                     /gene="trxA"
                     /locus_tag="VHM69_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2741056.1"
                     /translation="MAVAEVTDATFDSDVLRSEKPVIVDFWAPWCVPCRRVSPILEEM
                     AESREDVRFVKLNVDDNPSTAMNYSISSIPTIARFEEGQVTQQAIGALPKNQLSAQLG
                     L"
     gene            4238..4507
                     /gene="groES"
                     /locus_tag="VHM69_11470"
     CDS             4238..4507
                     /gene="groES"
                     /locus_tag="VHM69_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2741057.1"
                     /translation="MKFKPLGERALVQIVEREQQTESGIVLPDTAKEKPQTAEVVAVG
                     QFENGVKVSEGDVIVFAKYSGTEINLDGEDYMILDADDILGIVES"
     gene            4611..5681
                     /locus_tag="VHM69_11475"
     CDS             4611..5681
                     /locus_tag="VHM69_11475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2741058.1"
                     /translation="MRTLRRGDRGREVVDLQTRMQALGFDLGSRGIDGVFRERTELAV
                     RALQQGLGLLADGLVGPITWREVVEAGYRPGGRLLYLRQPPFRGADVVDLQQMLDDLG
                     FDPGAVNGLFDSRTVRAVRDFQKNAGLQDDGVVDAAVFKVLNTYAAQTLGTHQLPDKS
                     GGYFTDDLFSGAIVVDAAHGGRETGYVSATGLSEADLNLAVAQELAQILPAREVLLTR
                     SEDEEVSAADRAFLANSSGARMIVSIHHAWHPTPIACGTASFYFERLGYQSHRGRMAA
                     VYLQRSVSQTLGTCDIGEFGRSFEILRETNIPAVVLEPLHLTNPGDLDLASDPYYPVT
                     AAEAMASALELYAGRDKAFIAV"
     gene            5824..5897
                     /locus_tag="VHM69_11480"
     tRNA            5824..5897
                     /locus_tag="VHM69_11480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5858..5860,aa:Pro,seq:cgg)
     gene            complement(6060..>7688)
                     /locus_tag="VHM69_11485"
     CDS             complement(6060..>7688)
                     /locus_tag="VHM69_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2741059.1"
                     /translation="ISAFEPLMAREKELEELGARLSDDPSDALMQRYGRLQTEVERDG
                     GYEYRARAASTLSSLGFAPEDWKRPVGSFSGGEQSRIALARLLLEEPDLILLDEPTNH
                     LDLRAIEWLESFVKGAKSAVLVVSHDRYFLDAVAGSILELDEGRLHRYAGNYSKYVTE
                     KKAREEQLARKAKANAERRAQLERFVEKNRAKASKASQAKSKQKLLDRMEKIEDPKGA
                     SKNVRLDLGGVTARAGRVVLEMEDVRYAHEDSDQALLEGLDLAIERGERVALLGPNGT
                     GKSTIMRLATGELSPQRGMVRSGNNVTLAYQDQQLARLDDSKTVLQETMDTTGFDAPE
                     ARDLLGAFLFSGEDVFKKVSSLSGGERNRLSLAEIVVSGANVLLLDEPTNNLDIPARE
                     ALEEALLEYRGTMFFISHDRYFLRKLATRVVDLEGKKLINYLGGYDYYRSHRRLDENS
                     KSGKGRPRRRVRPGQSKEQSILATRLVAVEGEIDATERRVARLEKELATSELYSDSKR
                     SREVVTEHRQLKSMLDALYTDWGQLLEEAEEAGL"
BASE COUNT         1370 a         2416 c         2511 g         1391 t
ORIGIN      
        1 ttgcaggccg cgttcttcga gcgtctcggg cccgctggga tggggatcta tgcgaagctc
       61 atagaagccg ccgcggacct ggatctgccg gtgatagccg acgtcaagcg cggggatatc
      121 gggcccgtcg ccgccgccta cgcggaggcg catctcggcg tctacggcgc catctgcgtc
      181 acggtcaacc cttatatggg ggaggacgcg gtgctcccgt tcctcgaaga ggcgcgcaga
      241 cgaagcgtgg gcggaggtgt cttcgtgctt gtcgcgacct cgaacccgtc ctcgccagct
      301 attcaagaag cgacctcacc gccgctcttc gaggtcgccg ccggactggt tgcggagttg
      361 gggactacgg gcggcgacta ccctgatgcc ggtgcggtcg tcggggtcac gcggcctgag
      421 atcgggggcc gggtgcggga attgctgccc gacgcgctgt tcctctcccc cggttacggg
      481 gcacaggggg gcgaggcatc aggcgtacgc ccgctgctcg accggcgcgg aggcggggtt
      541 ctcgtcaaca gcagcagggg catcctctac gcctacgaag gatcgacctc aggctacagg
      601 gaggccgccg gggaagccgc ccgtcgggca cgggacgcgt tgtgccgggc aggagcccgc
      661 atgtagagct gtcagcccgc cgcccgactt cgccaggcgg ctctcagcca tcagctttca
      721 gcaagaacaa tttaagctga atgctgctaa ctgactgctg acggcgcgat ttgcgtagcg
      781 aatcgcgtca ggcggagaac aagtggacga cggcgagcac ggtgaccagc accgtcgccg
      841 cggcgaccac aacacctgcg atctgtacca tgctctctct cgtcatcttc tccttccggg
      901 gagcacgcgg gctccggaac tatctctctc cgcggagggc gctctggccg cccttctcgg
      961 gcttcttcgc gagggtctcg tccagggctt cactaatccc cttctcgtag ccatcgtaat
     1021 agtagtccag tgtgtactct accgtgccgt cctcgaccac gaacagcctc ggtactctct
     1081 tgacgttgta ctttgagacc agctcaccgg tcgagtcaag gagcatggtg tatggaacgc
     1141 cgtcttcgct cggggcgctg ttgacgtaga ccgccgcgaa ggagaccccg tcgtgctcgt
     1201 actccctggc gagatcttcg aagccaggaa ccgcctgatt ggagtacttg ttcagggtgc
     1261 tccagaacgt aagcacgtag acaccgtcgt cggagagctc gaagctgcgg ccctccacgc
     1321 tcttggcggt gaactcgggt gcatggtcgc caacgttcgg agccggttgg ggtagcgtga
     1381 gcgagcccgt gcccgtctcg gcgctgccga aggaacggta gagcagcgcc gcggccacca
     1441 ccatgaagac gacgatggcc aggcgtttca cgaaccctcc gaaccggatt tccaaagata
     1501 cacgacgcaa agattgtatc cgctagcgtt gcccttgtcg tgaggccaga atcatcgtag
     1561 cctctatagt cccgtgctga aaatcgccga caccaggatc acgaagcgct tcgtcgcctg
     1621 catccttgtg ctcctcctcg gcgcccaggt cggagccggc gcggcgtttg cgcagggggc
     1681 cagggaggtg gctgagcctg atgtggctgc gcgggcgtgg gccctgacgg acctcaggag
     1741 cggcgagtac ctggcagggg agaagtcctc aaagaagctc cccatagcct cgacgaccaa
     1801 gatcatgtcc gccctcgtgg tactgaagca gggcaacctc gacgaggagg ttaccgtctc
     1861 cgaggaagcc gccgcctacg ctaccccggc ctatagcaac gtcgggctcc tgtcaggcga
     1921 cacattgagc gtaagggagc tactgatggc aaccctgatc tcctcgggag acgacgccgc
     1981 atacgccctg gctgaacacg tcggcggcgg agggggcgtg gaccgtttcg tcgcagagat
     2041 gaacgaggag gcaaagaccc tcgggctcga agacacccac ttcgagaacc ctgtcggctt
     2101 cgacgccagc gcccactatt ccagcgcgcg agaccttgcc gagatggccc gcatggcgat
     2161 gcggaatccg gagttcaggg agatagtctc gaccgagtac gcgaccatct acaccccgta
     2221 cagggagata ccgctcgcca acaccaacga gttgctttat tcctacgggc cggccacggg
     2281 cgtcaagacg ggtacgaccc ctgccgcggg ggagaccctt gtgtcctcgg ccacgatcgg
     2341 ggacgagtcg tacgtttgcg tggtcctcga ctctcgcgaa gaccgcttcg cgggctcggt
     2401 acggaccctg cgatacgggt tcgctgccca cgaccgcgcg aatctcgtcg tcaggggcaa
     2461 acgttacgcc ggcgtcgacg tgccatacag gcgggacaag agcgtggatc tcctggctga
     2521 ggacgatgtc gatggcctcg ttgacgcgag ccctgacgtg gagcgcgacg tggagatagt
     2581 caaggacctg ccagactcgg ccagggccgg caccaggctc ggcgaggtcg acgtaagggt
     2641 ggatgggaag aaggtcgggg agacccctct ggtcgccaga accggctacg aaaaggcgcc
     2701 gccgtggcag cgggtgtggt ataccgttga agggattttc gagtaggaga tagggagagg
     2761 catgatactg accatcaccc tcaacgctgc cgttgcccgc acgcttgtcg tcccgagcct
     2821 gacgctcggc cacaggcaca gggcgccgga gagcataacg ctggcagggg ggaagggcgt
     2881 gaacgtggcc cgatccctga ggaccctgga tgtgcccgtc ctcgcggcgg ggttcgcagg
     2941 cgggcgcaac ggcgacgcca tacgcgacgg cctctctgag gcggccatcc cgttcgacct
     3001 cgtcgagata gagggatatt cccgtacatc gaccgccata atcgacccga tggccgggac
     3061 gcagaccgag atcaacgagt acggccccga ggtgacccag gccgaggcca aggagttctc
     3121 aaggcgcctg gagcacctga tggagtacgc tacggccgtc gtcttcgccg gaagcgtccc
     3181 gcccaacctg gacgagagct tcgtcgtcgg cctcgtgcgg cgcgcgagag agcgtggact
     3241 ctacaccgta gtcgactcgc ctccgtccgt gctcacggcc gccctcaagg ctaacccctc
     3301 actggtcagc ccgaaccagc acgaggcgga gagcgtcgtg ggcttcgact ttatcgaaga
     3361 cggagacttc cccctcggcc tctccaggct ggtcgagttc ggggccgcgg acgcatgcat
     3421 cacctcgccg gagggcctct cctacctgag caccgagaac ggcgtcgtct cggcccgcgc
     3481 gccaaaggtc gagaccgtct ccaccgtggg gagcggagac gcgtatctcg ccgggctcct
     3541 cgcgggactg ttgcaccgta acctcgcgcc tgtcgaggcc gtccggcttg ctgcagggtg
     3601 cgccgcagcc aacgccgaga ccctcggcgc tggactcttc gaggccagac gggccgaaga
     3661 gctggctgac tcggtgcagg tcgaggtcat ctcgcgcagt ctcgaacaga gcaggaccta
     3721 gagtactttc acgggctccg agatgcccgc actcgcgtgc tagaatatcc cggcgaagac
     3781 cgctttctag agattacgga ggttcgagaa catggcagta gccgaggtaa cggacgctac
     3841 tttcgacagc gacgtactac ggtcggagaa gccggttata gtggatttct gggcgccctg
     3901 gtgcgtacct tgccggcgcg tctccccgat cctggaagag atggcagagt cgcgcgagga
     3961 cgtgaggttc gtcaagctga acgtggacga caacccctcg accgcgatga actacagcat
     4021 atcgagcatc ccgaccatcg cccgtttcga ggaggggcag gtcacccagc aggcgatcgg
     4081 agccctgccg aagaaccagc tctcggcgca gctcggcctt taaactttgc gaggagcagc
     4141 cctcgcggtt acaatctctg gcgcataaaa gctctgcgag gtcgtttgcc gcgcggggcg
     4201 ccgtagaaaa gtccgaagat acgaggaggt caaacagatg aagttcaagc ctttgggaga
     4261 gcgggcgctc gtccagatcg tcgagcgcga gcagcagacg gaatccggga tagtgctccc
     4321 cgataccgcc aaagagaagc cgcagacggc cgaggtcgtc gctgtcggcc agttcgagaa
     4381 cggggtcaag gtcagcgagg gtgacgtcat cgtcttcgcc aagtactcgg ggaccgagat
     4441 caacctcgac ggcgaggact acatgatcct cgacgccgac gacatcctgg gtatagtcga
     4501 gagctagagg acgaaaagtc ggctctcagg agcgccgggc atcctcgcgg ggcccggcgc
     4561 tcccgcttta gccgactccg agggacggcg aaggggagac ggtcaggtac ttgcgtacgc
     4621 tgcgtagggg agacaggggg cgggaggtcg tcgatctcca gacccgcatg caggctctcg
     4681 gcttcgacct tgggagcagg ggtatagacg gcgtcttcag ggagaggacg gagctcgccg
     4741 tgagggcctt gcagcagggc ctcgggctcc tcgccgacgg gctcgtcggt ccgatcacct
     4801 ggcgcgaggt cgtcgaggca ggctacagac cggggggacg ccttctctac ctcaggcagc
     4861 ctcctttcag gggagccgac gttgtggatc tccagcagat gctcgacgac ctcggcttcg
     4921 accccggcgc cgtcaacggc ctcttcgatt cccgtaccgt cagggccgtg cgcgacttcc
     4981 agaagaacgc agggttgcag gacgacgggg tggtcgacgc cgcggtcttc aaagtcctga
     5041 acacctacgc cgcccagact ctgggcaccc accagctccc cgacaagagc ggaggctact
     5101 tcacggatga cctcttctcc ggggccatag tcgtcgacgc ggcgcacggc gggagggaaa
     5161 ccggctacgt cagcgcgacg ggactctccg aggctgacct caacctcgcc gtggcgcagg
     5221 agctggccca gatcctgccg gccagggagg tccttctgac ccgtagcgag gacgaggaag
     5281 tatccgcggc cgacagggcc ttcctcgcga actcctcagg ggcgaggatg atcgtctcca
     5341 tccatcacgc ctggcacccg accccgatcg cctgcgggac cgcctccttc tacttcgagc
     5401 ggctcggcta ccagtcgcac aggggccgaa tggccgcggt ctatctgcaa cgaagtgtca
     5461 gccagacttt aggtacctgc gatatcgggg agttcggaag gtcgttcgag atcctgcgcg
     5521 agacgaacat ccccgccgtg gtactcgaac ccctgcacct gaccaaccct ggcgacctcg
     5581 acctcgcatc agatccctac tacccagtaa ccgccgccga ggccatggca agcgccctcg
     5641 aactctacgc aggccgcgat aaagcattta tagctgttta gtaggtttca ggtttcaggc
     5701 atcaggtagg ggccgctcat ggcgtctccg ggggttcgtc tgtgggcgtg gatttgtagc
     5761 cactgggttg ccctctcaag gacctgaaac ctgaaaccta gctgatacaa ttaacgccac
     5821 agtcgggacg tagcgcagcc tggtagcgcg cctcgttcgg gacgaggagg tcggaggttc
     5881 aaatcctctc gtcccgactt ttgtaggttt cagccgtcag gcttcagggg ctcgatggac
     5941 tgtcattccg tggcattaga aaccctcggc caggcccatg tttgtttcgg tcgtacaaga
     6001 acgttgcgct gattagttga gttcggtgct ggtcacctga tgcctgtagc ctgaagcctt
     6061 caaaggcctg cctcctctgc ctcttcgagt agctgtcccc aatcggtgta caaggcgtcg
     6121 agcatgctct tcagctggcg atgctcggtg acgacttcgc gggagcgctt gctgtctgag
     6181 tagagctcgg aggtggcgag ttctttttcg aggcgggcta cgcgacgctc ggtcgcgtcg
     6241 atctcgcctt ccacggcgac gaggcgggtg gcgaggatgc tctgttcttt gctctggcca
     6301 ggacggacac gccggcgcgg gcggcccttg ccgctcttag aattctcatc cagcctgcgg
     6361 tgggagcggt aatagtcgta gccgccgagg tagtttatga gcttcttgcc ttccagatcc
     6421 acgaccctgg tcgcgagctt gcgcaggaag tagcggtcgt gggagatgaa gaacatcgtg
     6481 ccgcggtatt ccagaagcgc ctcttccagg gcctcgcggg cagggatgtc caggttgttc
     6541 gtcggctcgt cgagcaggag cacgttggct ccgctcacca cgatctcggc caggctcagg
     6601 cggttcctct cgccgcccga gagtgaggag accttcttga agacgtcctc gccggagaag
     6661 aggaaggcgc cgaggagatc tcgggcttcg ggggcgtcga agcctgtcgt gtccatcgtc
     6721 tcctgaagga cggtcttcga gtcgtcgagg cgggcgagct gctggtcctg gtaggcgaga
     6781 gtcacgttgt tgcccgagcg gaccataccc ctctggggag agagctcacc agtggccagg
     6841 cgcatgatcg tgctcttccc cgtaccgttg ggaccaagca acgccaccct ctcaccacgt
     6901 tcgatggcga ggtcgaggcc ttccaggagc gcctgatctg agtcctcgtg tgcgtagcgc
     6961 acgtcctcca tctcgagcac gacgcgcccc gcacgcgcgg tcaccccgcc gaggtcgagc
     7021 ctcacgttct tcgaggcgcc tttggggtct tcgatcttct ccatcctgtc gagcagcttc
     7081 tgtttgctct tggcctggct tgctttgctc gccttggccc tgttcttctc gacgaagcgc
     7141 tctagttgcg ccctgcgttc agcgttggcc ttcgccttgc gggcgagctg ctcctcgcga
     7201 gcctttttct cggtcacgta cttgctgtag ttgcccgcat agcggtggag gcggccttca
     7261 tcgagctcca ggatcgaacc cgccaccgcg tcgaggaagt agcggtcgtg tgagacgacg
     7321 aggacggcgc tcttcgcacc cttgacgaag ctctcgagcc actcgatggc gcgcaggtcg
     7381 aggtggttcg tgggctcgtc gaggagaatg aggtcgggct cctccagcag caggcgggca
     7441 agcgcgatgc ggctctgctc gccgcctgag aacgagccga cgggacgctt ccagtcctcg
     7501 ggggcgaagc ctaacgacga gagcgtcgag gccgcccgcg cgcggtactc gtagccgccg
     7561 tcgcgctcga cctcggtttg taacctgccg taacgctgca tgagcgcgtc cgacgggtca
     7621 tcggagagac gcgccccaag ctcttccaat tccttctctc gggccatcaa cggctcgaag
     7681 gcggagat
//
LOCUS       DATAIR010000475         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_123253, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000475 DATAIR010000000
VERSION     DATAIR010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123253"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VHM69_11490"
     CDS             complement(<1..1139)
                     /locus_tag="VHM69_11490"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2741060.1"
                     /translation="MQNNDIIRALETIATLMEIKDEPYYRVLGYQRASESVAALGRPV
                     TEVEDLKTLPHVGATTAEVIRDLAEDRTPRLLAELSAEIPTGLVEMTRLPSVGPRTVG
                     RLWKELGVTNVEELAAVEPERIASLKGFGKKSAEKMVHAARTYNATERRMLLDDASSM
                     GEQILSFVRSHPATERAEVGGSLRRMKETIGDIDIVAASEDPDSLADDFAEAPFADEV
                     LAHGPKKVFILSGGTEVDLRIVEPEAYGSLLHHFTGGQAHNIALRERAVKMGINISEY
                     GLAEAGTGDYKPVATEEDLYSRLGLAYIPPELREDAGEIEAAEKGRLPDLVSVEDIRG
                     DLHVHTNYSDGKGTIESMAEAAIELGYEYLVFCDHSQSLRVANGLS"
     gene            1211..1930
                     /locus_tag="VHM69_11495"
     CDS             1211..1930
                     /locus_tag="VHM69_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2741061.1"
                     /translation="MVKGRREIVRWLADRLTVVAAVWLAAGLAFSAFVIWAFAELADA
                     VVEGESRRIDRALLLWIHSTFPGWLDDPMRAVTALGYYWVVLPLLAVVFFLFYRRGWR
                     LSAVLLVVSTAGSILLTTILKAVFARTRPELFDSGYHASFYSFPSGHATLAVGFYGML
                     TLILAYRLRGIARWVVAVSGVIVVLLIGFSRLYLGVHYPTDVLAGYLSALLWLVCVGA
                     VYVLWLSIRGLRAAESSRDDE"
     gene            1943..>2446
                     /locus_tag="VHM69_11500"
     CDS             1943..>2446
                     /locus_tag="VHM69_11500"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2741062.1"
                     /translation="MDSILHLIAQYGYLIILFGVMAESTGVPLPGETILISAGILVQR
                     GGLDLGDAIFFGILGAVVGDQIGYWIGREGGRPFVLRWGRYVFVTPERLARAEAFFAR
                     HGGKAVFMARFFSGLRVFGALVAGMSRMRWGTFIFFNALGGAVWATAAVLVGYFLGSS
                     LGVVERWT"
BASE COUNT          337 a          776 c          803 g          530 t
ORIGIN      
        1 gagaggccgt tcgcgaccct gaggctctgc gagtggtcgc agaagacgag gtactcgtag
       61 ccgagctcga tggcggcctc ggccatgctc tcgatggtgc ccttgccatc tgagtagttg
      121 gtgtggacgt gcaggtcccc acggatatct tcgacggaga cgaggtcggg aagcctgccc
      181 ttctccgcgg cttcgatctc tcccgcgtcc tcccttaact ccggcgggat gtaggcgagg
      241 ccgagacgcg agtagaggtc ctcttcggtg gcgaccggtt tgtagtcccc ggtccccgct
      301 tcggccaggc cgtactctga gatgttgatg cccatcttca cggccctctc gcgcagcgcg
      361 atgttgtggg cctggccgcc tgtgaagtgg tgcagcagcg agccgtatgc ctctggctcg
      421 acgatcctca ggtccacctc ggtcccgccg gagaggatga agaccttctt cggaccgtgg
      481 gccaggacct cgtcggcgaa aggggcctcc gcgaagtcgt cggcgaggga gtcggggtcc
      541 tcgctcgctg cgacgatgtc gatgtcgcct atcgtctcct tcatgcggcg cagggagccc
      601 ccgacctcgg ccctctccgt ggctgggtgc gagcggacga aggagaggat ctgctccccc
      661 atagagctcg cgtcgtcgag gagcatccgg cgctccgtag cgttgtaagt ccttgcggcg
      721 tgaaccatct tctccgcgct cttcttaccg aagcctttta gagaggcgat ccgctccggc
      781 tcgacggccg caagctcctc gacgttcgtg acgccgagct ccttccacag ccgcccgacc
      841 gtgcgcggcc ccacgctcgg aaggcgcgtc atctcgacga ggccggtcgg tatctcggcc
      901 gacagttcgg cgaggagccg gggcgtcctg tcttcggcca ggtctctgat cacctcggcg
      961 gtcgtcgcgc cgacgtgggg tagtgtcttc aggtcctcca cctccgtgac ggggcgtccg
     1021 agggcggcca cggactctga cgccctctgg tagccgagca cgcggtagta gggttcgtct
     1081 ttgatctcca tgagcgtagc gatagtctcc agcgccctga tgatgtcgtt gttctgcaac
     1141 cgcatctcct tccgtccgcc acggagatta tacgcgccgc ccgagtcccg aaacgggcta
     1201 gaatctatcc atggtcaagg ggcgaagaga gatagtgcgg tggctcgcgg accggctgac
     1261 cgttgtcgcg gccgtctggc tggctgcggg gctggccttt tcggccttcg tcatctgggc
     1321 cttcgctgaa ctcgcagacg cggtggtcga gggcgagagt cgccggatcg acagggcctt
     1381 gctgctctgg atccactcaa ccttccccgg ctggctggat gatccgatgc gtgcggttac
     1441 cgcgctcggc tactattggg ttgtgctgcc gctcctggcc gtggtgttct tcctgttcta
     1501 ccgcagaggc tggaggctct ccgccgtgct gctcgtcgtc tcgacggcgg ggagtatcct
     1561 gctcactacc attctcaaag ccgtcttcgc gcggacgcgc cccgagctct tcgactccgg
     1621 atatcacgcc tccttctatt ctttcccgag cggacacgcc accctcgccg tcgggttcta
     1681 cgggatgctc acgcttatcc tggcttacag gctgaggggg atagcgcggt gggtcgtcgc
     1741 cgtctcagga gtcatcgttg tgctcctaat cggcttttcc aggctctacc tcggcgtaca
     1801 ctacccgacg gacgtgctgg cgggctacct ctcagctctc ctgtggctgg tctgcgtcgg
     1861 cgccgtgtac gtgctgtggc tctcgatccg tggcttgagg gccgccgagt caagccgcga
     1921 cgacgagtag tatcatccgt tagtggatag catcctccac ctcatcgccc agtacgggta
     1981 cctgatcatc ctcttcggcg tgatggccga gagcacaggg gtgccgctgc ctggcgagac
     2041 gatcctgatc tccgctggca tcctggttca gaggggcggc ctggatcttg gagatgcgat
     2101 cttcttcggc atcctcggcg ctgtggtggg ggaccagatc ggctactgga taggccgcga
     2161 agggggacgc cccttcgtcc tcaggtgggg acgatacgtc tttgtcaccc ccgaacgcct
     2221 cgcgcgcgcc gaagcgttct tcgccaggca tggggggaag gccgtattca tggcgcgctt
     2281 cttctcaggt ctcagggtat ttggagcact ggtcgcgggg atgagccgta tgcgctgggg
     2341 gacttttatc tttttcaacg ccctcggggg agcggtgtgg gccacggcgg ccgtcctggt
     2401 tggatacttc ctcggtagca gcctcggtgt cgtggagcgc tggacg
//
LOCUS       DATAIR010000476         7243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_123325, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000476 DATAIR010000000
VERSION     DATAIR010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123325"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHM69_11505"
     CDS             <1..288
                     /locus_tag="VHM69_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2741063.1"
                     /translation="EDVDITLHNGVLTISGERRADEQREGSGYYVRERRYGSFRRSMS
                     LPQGIDESRINARFDGGVLEVRVSGAAAVEEPRRIQIESGDESSGNGSSGG"
     gene            383..571
                     /locus_tag="VHM69_11510"
     CDS             383..571
                     /locus_tag="VHM69_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741064.1"
                     /translation="MERARVEAEYRRVLKSSVEFEKRIRDRWRAKCRAKNARIAELEE
                     RVAELERRLQESRAPQGG"
     gene            complement(568..2712)
                     /locus_tag="VHM69_11515"
     CDS             complement(568..2712)
                     /locus_tag="VHM69_11515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2741065.1"
                     /translation="MLRQPQITDAAIGGKGQGRRAHATSGDQEVRLPYRPGLDGLRAL
                     AVIAVLLYHADLAWIPGGFLGVEVFFVISGYLITALLLAEWRQRGRIDLKTFWMRRAR
                     RLLPGLYVLLVATLAFAVVFLPKEVAGLRGDVLAAFGYITNWYLIFGQESYFESVGRP
                     SLLQHLWSLAVEEQFYLLWPPILALGLGLGATRLRRRRVLTIALVGATVSAVAMALLY
                     TPGVDPSRIYYGTDTRATGLLFGAALAFLWSPGERYRPAESRHHRLNLPDRGRFRRRW
                     GWTAPLLLDIVGLVALGGLVWFCLHLGEFQSFLYRGGFALVALTTAAAIMAAVHPHTR
                     IGARLLGSAPLRWVGVRSYSIYLWHWPVFMVTRPELDVPIEGLPLLALRLTATVALAH
                     LSYRYIETPVRRGDLGRAWRTLRSARGPLRWHLRLQWAGVVLPILASCALLGVAVTQA
                     KPPEKPDYLASMKAVHTVEQSPHSSSISEIDSPSSDKPQEDNSKTPEKSARGNGESRD
                     EMKSEKSAESKARGDRPDKESNDAGAAPSVRAEKTAKDDRDGRRAGASEYTGSVSAIG
                     DSVMLGAVGRLQKDMQGLTVVDAEVGLQVYTAIDILEARSVSGQLGQVVIVHLGNNGT
                     FTKKQFDEIMQILSGVDRVVFVNVKVPRAWEEPNNEVIAEGVQRYPNAVLVDWHSASA
                     SQPGIFYSDGFHLRPKGQRIYADLISSYLPES"
     gene            complement(2855..3151)
                     /locus_tag="VHM69_11520"
     CDS             complement(2855..3151)
                     /locus_tag="VHM69_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741066.1"
                     /translation="MVDRETKERKLIALMVNDLGPGFGESEMAKLEEEVRALPDDELH
                     RTLVGRLDLANPADEESLEQALLHVEEPEELSGPEGFTGAPDMSHEPRPEEREG"
     gene            complement(3208..4974)
                     /locus_tag="VHM69_11525"
     CDS             complement(3208..4974)
                     /locus_tag="VHM69_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX2741067.1"
                     /translation="MRILDERREALVERERGLLERLISFLKDFGAPSEDVGLVRRALA
                     DLEELFLLVIVGEFNSGKSAFVNALLGADIAAEGVTPTTDRITVLRHADEPVEKERRD
                     GVLERGHPNEFLREIAIVDTPGTNAIIRHHEELSRGFVPRSDLVLFVTSAERPLTESE
                     RGYLELIRDWGKKILLVINKADLLGDESNVDEVRSFVDKGIRSALGLTPPIFFVSALL
                     ARKAKAATSAMERDALMKASGFAELESYIADLLDEEGRVRLKMESPLGVVDELNRRYR
                     SAVDERLSLLEDDFRTAENVESQLALYKEDMGRDFEARMAEIENIVLRMNERGDAWLE
                     ENIRFANLRELFRQEKVSERFKREVVGDTEDLVDVRVDELIDWMVDRNLKQWRMVVDY
                     VNRRRQANYDERLIGEVGDSFEYNRGQLLQSVGKNAQDVVHRYDREHESEKLAHSIQG
                     AVAQTAALEVGAVGIGAVVVALATTRILDATGIIAAAIIAGYGLFILPNRRRKARAEF
                     REKTGSLRERLGEVVRRQFETELNRSVERMREAIAPYTRFVRSEHARMTRAGEDLAAL
                     DDETSALKDEISAPGVGPKTSP"
     gene            5087..6055
                     /locus_tag="VHM69_11530"
     CDS             5087..6055
                     /locus_tag="VHM69_11530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2741068.1"
                     /translation="MNYRKLGRTGLEVSEIGYGAWGIAGDAWLGAKDEESLKALNRAV
                     DLGLNFIDTALAYGGGHSERLVGKLVEERDETIHVATKVPPKNRIWPAPEGVPVDEVF
                     PGDYVRECTDQSLSNLGLDAIDVQQFHVWQDDWVGQGDWLEAVEDLKQEGKIRSFGVS
                     INDHQPANAVRLIETGVVDTVQVIYNVFDQSPEDVLLPACQEHGVGVIVRVPFDEGAL
                     TGRITPETTFDEGDFRNSYFRGERKQEVHDRVRAIVSELGTSEDEIAEVALRYILSHP
                     AVSSVIPGMRSVRNVERNMAVGDGKGLPEDQVQRLKAHRWIRNYYA"
     gene            complement(6229..>7243)
                     /locus_tag="VHM69_11535"
     CDS             complement(6229..>7243)
                     /locus_tag="VHM69_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2741069.1"
                     /translation="ARVAIACQGGGSHTAFTAGALKRLLADERHEVVALSGTSGGAIC
                     AVLAWYALLEKGGAGRAAELLDSFWRNNSARDFLEAFWNEWFLWVNRSQGSFVLPAVS
                     PYDTPTSDWAQNQLRELLTDHVDFRKLKSLSKKSSPMLLIGAVDVLSGVFKAFSSRRD
                     EITAEMILASTALPTLFKAVHADGGVYWDGLFSQNPPIRELPRAKPDEIWILQIDPKK
                     RDEEPTSMTDILDRRNELAGNISLYQEVHFLQKMNHLVKKLGDPEVSPEERTLYVPGE
                     EGEEDREYKYIDIRWIRMSMPLDFASKLDRNPSFIRRLMSYGERRAEDFLEQRPTVPS
                     TGR"
BASE COUNT         1314 a         2269 c         2295 g         1365 t
ORIGIN      
        1 gaggacgtag acatcacgct gcacaatggg gttttgacca tctcggggga gcgcagggct
       61 gacgagcagc gggagggttc gggctactac gtcagggagc gccgttacgg ctcgttcagg
      121 aggagcatgt cgctacctca gggcattgat gagagcagga tcaacgcccg tttcgacggt
      181 ggcgtgctcg aggtgcgcgt ctctggcgcg gcggctgtag aggagcccag gcgcatccag
      241 atcgagtccg gcgacgaaag ctcgggcaac ggaagctccg gcggctaggc ggcctgggag
      301 cgcccgcagg agggggacag ggggcctctc tagggccccc tattccgctg gctagcggtt
      361 acacttgcga cgtatggagg acgtggagcg cgccagggtc gaggcggagt accggcgggt
      421 actcaagagt tcggtcgagt tcgagaagag gatcagggac cgctggagag cgaagtgccg
      481 cgccaagaac gcgcgaatcg ccgaactgga ggaacgcgtc gccgagctcg agcggcgcct
      541 ccaggagagt agggcgcccc agggcggcta gctttcggga aggtaggagg agatcaggtc
      601 ggcgtagatt ctctggccct tggggcggag gtggaagccg tcgctataga agatccctgg
      661 ctggctggca cttgcggagt gccagtcgac aagcacggcg ttggggtatc tttgcacccc
      721 ttccgcgatc acttcgttgt tcggctcttc ccaggctcgg gggaccttca cgttgacgaa
      781 cactactctg tccacgccgg agagtatctg catgatctcg tcgaactgct ttttggtgaa
      841 ggtaccgtta ttaccgaggt ggacgatcac cacctgtccc agttgtcctg agacgctgcg
      901 ggcttcgagg atgtcgatcg cggtatagac ttgcaggccg acttcagcgt ccacgaccgt
      961 gagcccctgc atgtctttct gtagtcttcc gacggccccg agcatcaccg agtcgccgat
     1021 ggcgctgacg gagcccgtgt actcggacgc gccggcacgc cttccatccc tgtcatcttt
     1081 cgccgtcttc tccgccctca cgctaggagc ggcgccggca tcgttggatt ctttatccgg
     1141 tctgtcccct ctagctttgg actcggcgga cttttccgac ttcatttcgt ccctggattc
     1201 tccgttacct ctggcgcttt tctccggtgt tttcgagttg tcttcctgag gtttgtcgct
     1261 cgaagggcta tctatctcgc tgatggaaga cgagtgggga gattgttcca cggtatgaac
     1321 cgccttcatg gatgcgagat agtcgggctt ctccggcggt ttcgcctgcg tcactgccac
     1381 gccgagcaga gcgcacgagg ccaggatggg caggaccacc ccggcccact ggaggcggag
     1441 gtgccagcgc agcggtcccc tggcactgcg gagcgtcctc caggcacgac ccaggtctcc
     1501 gcggcggacc ggcgtctcga tgtagcggta ggagaggtgt gccagcgcga ccgtcgcggt
     1561 caggcgcagg gcgagcaggg gcaacccttc gatcggaacg tcgagctcgg gtcgggtcac
     1621 catgaaaacc ggccagtgcc acaggtagat gctgtaagac ctcactccga cccagcgcag
     1681 cggagcggac ccgagtaggc gcgcacctat acgtgtgtgc gggtgcacgg cggccatgat
     1741 cgccgccgcc gtcgtcaggg cgacgagcgc gaatccaccc ctgtagagga aggactggaa
     1801 ctcgccaagg tgtaggcaga accagacaag gccgcccagc gcgaccaggc cgacgatgtc
     1861 gagcaggagt ggggcagtcc atccccagcg gcgcctgaac cgtcccctat caggaaggtt
     1921 gagcctgtgg tggcgtgact cggcgggacg atacctctcg cccggtgacc acaagaaggc
     1981 gagcgcggcc ccgaagagca agcccgtcgc ccgcgtatcc gtaccgtagt agatcctgga
     2041 cgggtcaacg cccggcgtgt acaggagggc cattgccaca gccgagaccg tcgcgccaac
     2101 cagcgctatc gtcagcacgc gccgccgccg cagtcgcgtc gccccgagac ccagcccgag
     2161 ggcgagaatg ggcggccaga gcaggtagaa ctgttcctct acggcgagcg accagaggtg
     2221 ttgcagtagc gagggccgcc cgaccgactc gaagtacgat tcctggccga agatcaggta
     2281 ccagttcgtt atgtacccga acgccgccag aacgtcgcca cgtagcccgg ccacctcctt
     2341 gggcaagaag acgacggcga aggccagggt ggcgacgagc agcacgtata gcccgggtag
     2401 gagcctgcgt gcgcgccgca tccagaacgt cttcaggtcg atgcggcctc tctggcgcca
     2461 ctccgcgagg agcaaagcgg tgatcaggta tccgctaatc acgaagaaga cctcgacccc
     2521 gaggaatccg ccggggatcc aggccaggtc cgcgtggtac aggagtacgg cgatcacggc
     2581 gagcgcccgc agtccgtcga ggccaggcct gtagggtagc cttacttcct ggtcgccgga
     2641 ggtcgcatgg gcacgtctgc cctgtccctt gccgccgatc gcggcgtcgg ttatctgcgg
     2701 ctgtcgtagc atgcgttccg tattttcccg ccctccactt ctgaggaaga tgatcctagc
     2761 acagtctccg gaggctatgc agggtcaaac ggacaaaaaa agcactacat gtagtactct
     2821 tcacggccac gccagatggt gtgacaggac ccggtcaacc ttcccgctct tcagggcgcg
     2881 gctcgtggct catgtcaggg gcgcctgtga agccttcggg gcctgagagt tcctcgggct
     2941 cttcgacgtg caagagggcc tgctccagac tctcttcgtc ggccgggttg gccagatcca
     3001 gcctccccac gagcgttcta tggagctcgt cgtcagggag ggcgcgcact tcttcctcca
     3061 gcttcgccat ctcactctct ccgaaacctg gccccaggtc gttcaccatc agggctatga
     3121 gcttgcgttc cttcgtctcg cggtcaacca ttctcgcctc ctcgaaagcg tccggcatcc
     3181 gagtctacaa cggataaagg ctggcgttca gggggacgtt ttcggcccga ctccgggggc
     3241 ggagatctca tccttcaggg cgctagtctc gtcgtcgagg gcggcgaggt cctcacccgc
     3301 tcgggtcatg cgggcgtgct cggagcggac gaagcgggtg tatggggcga ttgcctcgcg
     3361 catccgctcg acggagcggt tcagttccgt ttcgaactgc cgcctgacga cctcgcccag
     3421 acgctcgcgc agagagcccg tcttctcgcg gaactccgcg cgggccttgc ggcggcggtt
     3481 ggggaggatg aacagaccgt agcccgcgat tatggctgct gcgatgatcc ccgtggcgtc
     3541 caggatgcgc gtcgtggcga gggcgacgac gacggcccct atgccgacgg ccccgacctc
     3601 cagcgcggcg gtctgggcga cggcgccctg gatcgagtgc gccagcttct cggactcgtg
     3661 ctcgcggtcg tagcggtgga cgacgtcctg ggcgttcttg ccgacggact ggaggagctg
     3721 gccgcggttg tactcgaagc tatcgccgac ctccccgatc aggcgctcgt cgtagttggc
     3781 ctggcggcgg cggtttacgt aatcgacgac catgcgccac tgcttgaggt tcctgtcgac
     3841 catccagtct attagctcgt caacccgcac gtccaccagg tcctccgtgt cgccgacgac
     3901 ctccctcttg aagcgctcgc tcaccttctc ctgcctgaag agctcgcgga ggttggcgaa
     3961 ccggatgttc tcctcaagcc acgcgtcgcc ccgctcgttc atcctcaaga ctatgttctc
     4021 gatctcggcc atccgcgcct cgaagtccct gcccatgtcc tctttgtaga gcgcgagctg
     4081 cgactcgacg ttctcagcgg tcctgaaatc gtcctccagc aagctcaaac gctcgtccac
     4141 ggcgctcctg taacgtcgat tcagctcgtc caccaccccg agcgggctct ccatcttgag
     4201 gcgaacccgt ccctcttcgt cgagaaggtc cgctatgtag ctttcgagct cggcgaagcc
     4261 gctggctttc atgagggcgt cacgctccat ggcgctcgtc gccgccttcg ccttgcgggc
     4321 caggagggcc gagacgaaga agatcggggg cgtcaggccg agcgccgagc gtatgccctt
     4381 atctacgaag gagcggacct catcgacatt agattcgtcc cccaggagat cagccttgtt
     4441 gatgacgagc aggatcttct tcccccagtc gcggatgagc tcgagataac cgcgctcact
     4501 ctccgtcagt gggcgctcag cggaggtgac gaagaggaca aggtcgctcc tcggtacgaa
     4561 cccacggctc aattcctcgt ggtggcggat gatcgcgttg gtccccggcg tatcgacgat
     4621 ggcgatctca cgcaggaact cgttgggatg ccctctctcg aggaccccgt ccctccgctc
     4681 cttctcgacg ggctcgtcag cgtgacgcag cacggttatg cggtccgtgg tcggggtgac
     4741 gccctctgcg gcgatatccg cgccgagcag agcgttcacg aaagcggatt ttccggagtt
     4801 gaattcgccc actatgacga gcaagaacag ctcttccaga tcagcgagag cgcgcctgac
     4861 gaggccgacg tcctcagaag gcgcgccgaa gtccttgagg aaagagatca ggcgctccag
     4921 aagcccgcgt tctctttcca ccagcgcttc gcggcgctcg tcgagtatcc gcacttctaa
     4981 gtctccctct gcatgcaagc attatagacg ccagccggtt tcgtgaggcc cctctcgtgg
     5041 taacaaaaca cagaaccatc cggcaagcgg tatagatgga ggctctgtga actacagaaa
     5101 gctcggcaga actggtctgg aagtttctga gatcggctac ggtgcatggg gcatcgccgg
     5161 tgacgcctgg ctcggcgcga aggacgagga atcgctcaag gcgttgaacc gggccgttga
     5221 cctcggtctc aacttcatag acacggccct cgcctacgga ggaggacaca gcgaaaggct
     5281 cgtagggaag ctcgtcgagg agagggacga gaccatacac gtcgcgacca aggtccctcc
     5341 gaagaacagg atctggcccg cccccgaagg cgtcccggtc gatgaagtgt tccccggaga
     5401 ctacgtcagg gagtgcacgg atcagagcct ctccaacctg ggactggacg ccatcgacgt
     5461 gcagcagttt cacgtctggc aggacgactg ggtcggccag ggggactggc tcgaggctgt
     5521 cgaggacctg aagcaggagg gtaagatccg ctccttcggc gtctccataa acgaccacca
     5581 gcctgcaaac gccgtcaggc tcatcgagac cggcgtcgtc gatacggtgc aggtgatcta
     5641 caacgtcttc gaccagagcc ctgaggacgt actcttgcca gcgtgccagg agcacggcgt
     5701 cggcgtcatc gtgcgggtcc ccttcgacga gggcgccctg accggcagga tcacacctga
     5761 gaccaccttc gacgagggag atttccgcaa cagttacttc cgcggcgagc gcaagcagga
     5821 ggtccacgac cgcgtgcggg ccattgtctc cgagctcggg acgagcgagg acgagatcgc
     5881 cgaggtagct ctccggtaca tccttagcca tcccgccgtc tcgtcggtca tcccagggat
     5941 gcgctccgtg cgcaacgtcg agcgcaacat ggccgttggc gacggtaagg gactccccga
     6001 ggatcaggta cagcgcctga aagcccaccg ctggatccga aattactacg cgtagagcgg
     6061 tttgctggag gcacaagcgc ctccagcaaa ccagggctac aggctacagg tttcaggcat
     6121 caggaggttc tacagggaat ctcgtgcacc acgagagtct gcctgagcgt gctgcagctc
     6181 attcgatctg acgaacgctg tagggacccg ggagttgcct ttgaaaggct atcgtcctgt
     6241 ggatggtaca gtgggacgct gttcaaggaa gtcctcggcc cgcctttccc cgtagctcat
     6301 caaccttcgg ataaaagaag ggttgcggtc cagcttggag gcgaagtcga gtggcatgga
     6361 catcctgatc caacgtatat ctatgtactt gtactctctg tcttcctcgc cctcttcgcc
     6421 gggaacatag agcgtccgtt cctccgggct gacctctgga tcgcctagtt tcttgaccag
     6481 gtggttcatc ttctgtaaga agtggacctc ctggtagagc gagatgttgc cagccagttc
     6541 gttgcgcctg tcgaggatgt ccgtcatcga cgtcggttcc tcgtcgcgct tcttcggatc
     6601 tatctggagg atccagatct cgtccggctt cgcccggggg agctcgcgta tcggagggtt
     6661 ctgggagaag agcccgtccc agtaaacgcc cccgtctgcg tgaaccgcct tgaacagggt
     6721 aggaagcgcg gtagaggcga ggatcatttc ggcggtgatc tcatcccttc ggctgctgaa
     6781 ggccttgaac acgcctgaaa ggacgtcgac ggcgccgatc aggagcatcg ggctcgattt
     6841 tttggaaagg ctcttgagct tcctgaaatc gacgtggtcc gtcagcaact ccctcaactg
     6901 gttctgtgcc cagtcagagg ttggcgtgtc gtacgggctg acggcgggca gtacgaagga
     6961 gccctgcgac cggtttaccc agaggaacca ctcgttccag aaagcttcga gaaaatctct
     7021 cgcggagttg ttcctccaga aggaatccag tagctccgcc gccctgcctg ctccgccctt
     7081 ctccaacaac gcgtaccagg caaggaccgc gcagatcgca ccccccgagg tgccgctcag
     7141 ggccacgacc tcgtggcgct catccgccag caacctcttc aaagcccccg ctgtgaaggc
     7201 cgtgtggctc ccgccaccct ggcaggcgat cgcgacacgt gcc
//
LOCUS       DATAIR010000477         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_152675, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000477 DATAIR010000000
VERSION     DATAIR010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152675"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1558)
                     /locus_tag="VHM69_11540"
     CDS             complement(23..1558)
                     /locus_tag="VHM69_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX2741070.1"
                     /translation="MPRLLHGMDITPDTIRDARNGMAARAADVPLGFAVAQPLDTRRF
                     DFLLPGLQEDPANLLRVARKTRDDLVELGSAMGTVFGGDANIPAAYTYFGQFVDHDIT
                     LESSSATGSQLVAPDLTPLPLARIREETLNLRTGALDLDSVYGLPAPQDGARMRIGKV
                     SESGSRPRNKDDFNDLPRRRRIPDDPERDREALIGDPRNDENLIVAQLHLAFLRAHNE
                     LVDRGNSFRGARRLLRQHYQHIVIHDFLKRIVDPQIVDETIALNRVYDPAEDNLFMPL
                     EFSVAAYRFGHSMVRGNYDFNVNFQPATLEQLFTFTALSGQLGDFDTLPELWIIQWEN
                     FVDAGVPFTKARSIDTKLVEPLEHLRNLTGGEVAGLAKRLAVRNLLRGYLLRIPTGQA
                     VARALKEKLQGVRDIPVLGPNRIRQGVDSEGQVQVLEEAGFLERTPLWYYILAEAMEV
                     GGGQRLGPVGGTIVAEVLVGLVRRSANSILRQENWQPSLPSAQPGKFTLKDLLNFAGV
                     LPR"
     gene            2072..>2593
                     /locus_tag="VHM69_11545"
     CDS             2072..>2593
                     /locus_tag="VHM69_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX2741071.1"
                     /translation="MGRIRLTIHHLYADMMNLYGDRGNVISIKKRCEWRGIPVDVVDV
                     GLGEKIAPTGCDLFLFGGGQDREQALLAEDLSGSKGADLRAIVEDGGVVLGVCGGYQL
                     MGHSYETPEGEKLPGVGVFDLHTEPRKQDQERLIGNVLVRVISPETGETREVVGFENH
                     GGRTYLGAGEKPLG"
BASE COUNT          473 a          768 c          862 g          490 t
ORIGIN      
        1 gagaagcacc ggtggctaat agctaccggg gaagcacccc ggcgaagttc aacaggtcct
       61 tgagggtgaa cttacctggc tgggcacttg gcaagctggg ctgccagttc tcctgacgaa
      121 ggatggagtt cgcgctgcgc cggaccagtc cgacaaggac ctcggcgacg atcgttccac
      181 cgaccggccc caggcgttgg ccgccaccga cctccatcgc ttcggccagg atgtaatacc
      241 agagtggcgt gcgctccagg aacccggcct cctcgagtac ctggacctgc ccctcgctat
      301 ccacgccctg cctgatccgg ttcggtccga gcacgggtat gtcacgcacc ccttgaagct
      361 tctccttgag ggcgcgggca acggcctgac ccgtggggat gcgcaacagg tagccgcgca
      421 ggaggttccg cacggccagg cgcttggcga gcccagccac ctctccaccc gtcagattcc
      481 gcaggtgttc gagcggttcg accagcttcg tgtcgatgct gcgcgccttg gtgaagggta
      541 ctcccgcgtc gacgaagttc tcccactgga tgatccagag ttcaggcaaa gtatcgaagt
      601 ctccgagttg cccgctgagt gcggtgaagg tgaagagttg ttcgagggtc gccggttgga
      661 agttcacgtt gaagtcatag ttgccccgca ccatgctgtg gccgaatcgg taggctgcca
      721 cggagaactc cagcggcatg aagaggttat cttcggccgg gtcgtagacg cggttgagcg
      781 cgatcgtttc atctactatc tggggatcga ctatcctctt caggaagtcg tggatgacga
      841 tgtgctggta gtgctggcgc agtagtctcc tggcgcccct gaagctattg ccccggtcta
      901 ccagctcgtt gtgggcgcgc aggaaggcga ggtgcagctg tgcgacgatg aggttctcat
      961 cgtttctggg gtcgccgatc agggcctcac ggtcgcgttc gggatcgtct ggtatccgtc
     1021 tccgccgcgg gaggtcgttg aagtcgtcct tattccgggg acgactaccg ctctcggaga
     1081 ccttcccaat tctcatcctc gcgccgtcct gcggcgccgg caacccgtag acgctgtcca
     1141 gatccagggc ccccgtgcga aggttcagcg tctcttccct tatgcgggcg agcggaagcg
     1201 gggtgagatc gggggccacc agctgcgaac cggtcgccga ggaactttcg agcgtgatgt
     1261 cgtggtccac gaactgcccg aagtaggtgt aagctgccgg gatattggcg tccccgccga
     1321 agacggtgcc catggcactg ccgagttcga cgaggtcgtc gcgcgtcttg cgtgccacac
     1381 gtagcaggtt ggcggggtct tcttgtagtc cggggagcag gaaatcgaag cgtcgggtgt
     1441 cgagcggctg agcgacagcg aaccccaacg ggacgtcggc agcgcgagcg gccataccgt
     1501 tgcgggcgtc acgtattgtg tccggagtga tgtccattcc gtgcagcaag cggggcatga
     1561 tctggcgcct ccatgaatcg tgtgtttgca tgcctgcagg tccaatcacg aattgacttt
     1621 gcctctcttc tccctccttc ccgagaggag agctctcgcc actatgactt ctcgccgcgc
     1681 ttgtaaggcg cagcgtagcg tagcaagcag cgatgaatcg gcgatgaatc tggcaagaac
     1741 atccgacgca gcgctgcgac tgcgaggcac gcgaggacgt cccgcgcgcc aaacgtagca
     1801 acccgaccaa atcggagtta cgcttatggt cgcctggcac gaggagcgga tgccggtcgt
     1861 ccgagagtgt gagactatgt gcgcggagcg tactcccgag aacagtttcg gcccgtacaa
     1921 gctcacgccg ggcagattcg gatatttgtc ggagaaggag cgaggcaagg ggggcgcgag
     1981 gtttcgagat atctatacca ggtctccggg cagcggtcca cgaggacggt accctggaca
     2041 tcgcgatagg gaccacgaag ggagttttgc cgtggggcgg atcaggctca caatccacca
     2101 cctttacgcg gacatgatga acctctacgg cgacagggga aatgtgatct ccatcaaaaa
     2161 gcgatgtgag tggcgcggaa tccctgtcga tgtggttgac gttggcctcg gggagaagat
     2221 agcgccgacc ggctgcgacc tcttcctctt cggtggtggc caggacaggg agcaggccct
     2281 gctcgcggag gacctctcgg gctcgaaggg cgcggacctg agagctatcg tcgaggacgg
     2341 cggcgtggtc ctcggggtgt gcggcggcta ccagctcatg ggccactcct acgagacccc
     2401 cgagggtgag aagctccccg gcgtcggagt cttcgacctg cataccgagc cgagaaaaca
     2461 ggaccaggag cgcctgatcg ggaacgtgct cgttcgcgtc atttccccgg agacaggcga
     2521 gacgcgcgaa gtcgttggct tcgagaacca cggcgggcgg acctacctcg gcgccggcga
     2581 gaagccgctc ggc
//
LOCUS       DATAIR010000478         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_123699, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000478 DATAIR010000000
VERSION     DATAIR010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123699"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VHM69_11550"
     CDS             complement(<1..1770)
                     /locus_tag="VHM69_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX2741072.1"
                     /translation="MKKAGLASLERGRRLVREQLGRTAIAYAVLAVALLLTCLAYLYV
                     RHNVEERESAQFKETARATKQAVDRRMDTYIDATLDGRGLFAASNAVMREEWSSYVAG
                     SELERRYPGIQAIAYAERVPLEEKKSYEERVRAEGLSSFELRPSGERYEYFPLTYVAP
                     AQKANGPLLGYDVYSDRVSRAAMEQARDTGLPRASGKVDLGKAGNSRHSGFLVFTPVY
                     RHGAPDGTADERRSSLQGFIVSVFSTDELLGGIFGAQANPQVDFEIFDGAEFTRGGLL
                     HDDDGVLHAGDPSYEPRFQDTTTLEVAGRIWSLYFSTPSSSAPGWQSNLPLFVLLSGL
                     AISLMLFGITWVLSNNRIHAVRVGLELEAANEELEDANRDLEETNRELEGANRELEAF
                     SYSVSHDLRAPLRSIEGFSQILIEDYASHIDEEGRGYLHRVRASSRRMALLIDDLLDL
                     SRVTRTEMRRRSVDLSALARDISEELRKSRPWRKVEFEIEDGLVANGDARLLRVALWN
                     LLDNAWKFTAKEPNPRIELASTLRDGRRVYRVTDNGAGFDARYSDKLFGAFQRLHSSE
                     EFEGTGIGLATVARIVHRHGGEVW"
     gene            complement(1773..2216)
                     /locus_tag="VHM69_11555"
     CDS             complement(1773..2216)
                     /locus_tag="VHM69_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741073.1"
                     /translation="MKNKVILLVEDNPDDELLTLRALRRNGVTNEVVVARDGVEALDY
                     LFASGSHSGRDAGVMPELILLDLKLPRVDGFEVLRRLRSDERTRLLPVVILTSSKEQQ
                     DMLEGYGLGANSYVRKPVNFEQFVSAVEQLKLYWLVLNEAPPGGV"
     gene            complement(2307..2756)
                     /locus_tag="VHM69_11560"
     CDS             complement(2307..2756)
                     /locus_tag="VHM69_11560"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2741074.1"
                     /translation="MQEDPNLGGQREQVSDGSVGASSEAMSAQDERTWSIIAHLSVLA
                     GLVGLMPFGALIVWLLYKDRSPRVRFHALQALWYQIAWVVILVAYTFVSAVLSLVIIG
                     IFMFFLLPILALVPLAHGCYAAYQVSQGVEYRYPYIADRIEGPSSVV"
     gene            3034..>3522
                     /locus_tag="VHM69_11565"
     CDS             3034..>3522
                     /locus_tag="VHM69_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741075.1"
                     /translation="MGTPLYVLLVEDLEDDALLLVRRLRQGGYDPTWERVDTAATMEA
                     ALEERRWDLVISDHSMPAFSSSAALELLRRKGFDDLPFIIVSGQIGEDAAVAAMKAGA
                     HDYLMKDDLGRLTSAIERELREAGVRRERRRAEDALRVSETRFRLMIEQSPLSIQIFS
                     PDG"
BASE COUNT          599 a         1150 c         1115 g          658 t
ORIGIN      
        1 ccacacctct ccgccgtggc ggtgcacgat gcgcgcgacc gtcgcaagcc ctatccccgt
       61 cccctcgaac tcctctgagg aatgcagcct ctggaaggcg ccgaacagct tgtccgaata
      121 tcgcgcgtcg aagccggccc cgttgtccgt cacgcggtag acgcgccgcc catcgcgcaa
      181 ggtgcttgcg agctcgatcc tcgggttcgg ctccttagcg gtgaacttcc aggcgttatc
      241 caggaggttc cacagcgcga cccgcaggag acgggcatcc ccgttggcga cgaggccgtc
      301 ttcgatctcg aactccacct tgcgccaggg acgggacttc ctgagttcct cggagatgtc
      361 gcgggcaagt gcgctcaggt cgacgctccg gcggcgcatc tccgtgcggg tcacccgcga
      421 gaggtcgagg aggtcgtcta tgaggagggc catgcgtctg ctcgaggccc gaacccgatg
      481 gaggtagccg cgcccctcct cgtcgatgtg cgaggcgtag tcctcgatga gtatctggga
      541 aaatccctcg atgcttctca gcggcgccct caggtcgtgc gataccgagt atgagaacgc
      601 ttcgagttcc ctgttggccc cttcgagctc cctgttcgtc tcctcgaggt ccctgttggc
      661 gtcctcgagt tcttcgttgg cggcttccaa ctcgagcccg acgcgcacgg cgtgtatgcg
      721 gttgttggag agcacccagg taatgccgaa gagcatcagg ctgatcgcca gcccgctcag
      781 cagcacgaag agcggcaggt tactctgcca gcccggtgcg gagctcgacg gtgtgctgaa
      841 gtagaggctc cagatccgtc cggccacttc gagggtggtc gtgtcctgga aacgcggctc
      901 gtaggagggg tctccggcgt gcaaaactcc gtcgtcgtcg tgcagcagac cccccctcgt
      961 gaactcggcc ccgtcgaaga tctcgaagtc cacctgtgga ttcgcctggg cgccgaagat
     1021 gccgccgagc aactcgtccg tgctgaagac gctgacgatg aacccttgca gggagctgcg
     1081 tcgctcgtca gcggttccgt ccggcgcgcc gtggcggtag acgggcgtga aaacgaggaa
     1141 gcccgagtga cgcgagttcc ccgctttccc gaggtcgacc ttgcctgacg ccctcggcag
     1201 ccccgtgtcc cgcgcctgct ccatcgcggc gcggctcacc ctgtccgagt acacgtcgta
     1261 gccgaggaga ggcccgttgg ccttctgcgc gggtgcgacg tacgtgagcg ggaagtactc
     1321 gtagcgttcc ccggagggcc gtagctcgaa ggaggagagg ccttccgccc tgacgcgctc
     1381 ctcgtaggat ttcttctcct ccaacggcac gcgctcagcg taggcgatcg cctgaatgcc
     1441 gggataacgg cgctccagtt cgctgcctgc gacgtagctg ctccactcct cgcgcatgac
     1501 ggcgttgctc gcggcgaaga ggccccgccc gtcgagtgtc gcgtctatgt aggtgtccat
     1561 gcgcctgtcg acagcctgtt tggtggcccg tgcggtctcc ttgaactgcg cgctctctcg
     1621 ctcctctacg ttgtgcctca cgtaaaggta ggctaggcac gtcaagagca gagcgaccgc
     1681 cagcaccgcg taggcgatcg ccgtacggcc cagttgctcc cgcaccaggc gcctgcctcg
     1741 ctccagcgac gcgagcccgg ccttcttcaa ggctacacgc ctccgggcgg ggcctcgttt
     1801 agtaccagcc agtaaagctt gagttgctcg acggcactga cgaactgctc gaaattgaca
     1861 ggcttgcgga catagctgtt ggcgccgagg ccgtagcctt cgagcatatc ctgttgctcc
     1921 ttcgaagatg tcaggatcac cacgggcaac agccgcgtcc gttcgtcgga gcgcaggcgc
     1981 ctgagtacct cgaacccgtc tactctgggc agcttgaggt cgagcaggat caactcaggc
     2041 atgacgccgg cgtccctacc cgagtgggag ccgctcgcga acaggtaatc tagggcctcg
     2101 accccgtcgc gcgcgacgac gacctcgttc gtgacgccgt tcctcctgag cgcccgcaac
     2161 gtgagcagct cgtcgtccgg gttgtcctcg accaggagga tgaccttgtt cttcatctcg
     2221 cccctagccc gttagagaat tcggtcttta ccgaggaccc gcgtacctcg gtaaaaaccg
     2281 gaagttcctc catgccacag tcggcctcag acgacgctgc ttgggccctc tatcctgtcc
     2341 gcgatgtacg ggtagcggta ctctaccccc tggctgacct gataggccgc gtaacagccg
     2401 tgcgccagcg gcacgagcgc aaggatgggc agcaagaaga acatgaagat gccgattatg
     2461 acgaggctca atacggcgct gacgaaggtg taagccacga gaatcacgac ccacgctatc
     2521 tggtaccaga gcgcctgcag ggcgtggaag cgcaccctcg gggaacggtc tttgtagagc
     2581 agccagacga tcagggcccc gaaaggcatg aggccaacga gccccgcgag cacgctcagg
     2641 tgggcgatta tggaccacgt ccgttcgtcc tgcgcgctca tcgcctccga cgaggctccg
     2701 accgaaccgt ccgaaacctg ttcccgctga ccgccgagat tgggatcttc ttgcatcttg
     2761 gctccttacc tctcttcgtg tttgtttgca tacagctacg ggtgtcgcca tgatagccac
     2821 gcaatggacg cccgcgtatc ccccaaatga gtgattttct cgtgttttgc cccgtggcgg
     2881 agattgcctc gacgccctgg ggacaataga atacagactc gtgtgatgtg cgcgacgctg
     2941 atcatcgtga aggagcggtg ggacctgggc ccgccatgcg cgccgagtag catgggctgt
     3001 ggcttgatgg aacgtcgctg gaccggtgcc tgaatgggca cgccgctcta cgtcttgctg
     3061 gtcgaggacc tggaggacga cgccctgctc ctggtgagga ggttgcgcca gggcggctac
     3121 gaccccacgt gggagcgggt cgacaccgcg gcgaccatgg aggcggcgct cgaagagcgc
     3181 cgctgggacc tcgtcatctc cgaccattcc atgccagcct tcagctcgtc tgcggcgctc
     3241 gagttgctca ggcgcaaggg tttcgatgac ctgccattca tcatcgtgag cggccagatc
     3301 ggcgaggacg ccgcggtcgc ggccatgaag gccggagccc atgactacct catgaaggac
     3361 gacctcggca ggctcacgag cgccatcgag cgggagctca gggaggccgg ggtacgccgc
     3421 gagcgtcgga gggcggagga cgcgctgcgc gtctccgaga cgcgtttcag gctgatgatc
     3481 gaacagtccc ccttgagcat ccagatcttc tcccccgacg gc
//
LOCUS       DATAIR010000479         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_445888, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000479 DATAIR010000000
VERSION     DATAIR010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445888"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..509)
                     /locus_tag="VHM69_11570"
     CDS             complement(114..509)
                     /locus_tag="VHM69_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741076.1"
                     /translation="MRVYFFGNEHVAEDRRAIEAARELEGAIEGISFVFISPNEDVPF
                     ADERRVVILDTVQGIRDVALIEGVDGVILSPRGSVHDFDLAFQLRYLKKLGKLEEVTI
                     IGIPQEGEVDYLRIQSIFRELVAHDMQGS"
     gene            complement(541..3288)
                     /locus_tag="VHM69_11575"
     CDS             complement(541..3288)
                     /locus_tag="VHM69_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX2741077.1"
                     /translation="MSEDARVSLDPEERLDLLLGHLGTRRQGLSEREAARRLEQHGPN
                     EIRRRKSVSRLSEFARQFTHPLALLLWAAAALAFVGGTLSLSVAIVGVIVLNAVFAFF
                     QELQAEKATEALKQYLPPHARVRRAGGAVEVEATTLVPGDVLLLSEGDRLSADARLID
                     GSVEVDMSALTGESQPVARGAVRFRPASSPLESDDLLFGGTLVTGGEAEAVVYATGMA
                     TQFGRIATLTQRVKVEVSPLQLQVNRAARLISFVAVGAGVLFLTAGAALADLPLADAL
                     LFAIGLLVANVPEGLLPTITLALAVGVRRMARRRALLKRLTAVETLGSTDVICTDKTG
                     TLTEGRMILSRFWADGKEMDPKHDGSVGSEPLSGLLRTAVRCSNAGLRRANDGWERTG
                     DPSESALLAAAALLGEDVERAQEERGKRRRRLYHFDARVKRMTTADEEPDGSLWYHVK
                     GAPLELLERCTSVRGADGDKLLTDEDRRNVHVAFERYAGEGLRVLGFAEKKVSSPEPV
                     EDRDDAESKLTFLGLAALEDPPRPDVADAVSRCLNAGIRIIVVTGDHGLTAEAVARRV
                     GIVRGSPRIISGSEVDAMHQHELDTLLRDTPELIVARSNPETKLHVVDALRTSGHTVA
                     MTGDGVNDAPALRRADIGVAMGASGTEVARESATMVLTDDSFASIVAAVEEGRVVYDN
                     IRKFVTYIFAHATPEVVPFLIYALSGGAIPLPLTVMQILAIDLGTETLPALALGREPA
                     EPGVMQRPPRPRDRGIIDRPMLARAWGTLGLVEAALVTGGFFFVLYSAGWSPGEATGQ
                     GSHLHGAYLAATTMTFAGITACQVGTAFATRTSHASLREIGVFSNRLLLWGILFELVF
                     AAMVIYLPPLQAVFGTHALGLREVALLAIFPFVVWGTDEIRRWTLRRRERQAEGIRET
                     S"
     gene            complement(3317..3748)
                     /locus_tag="VHM69_11580"
     CDS             complement(3317..3748)
                     /locus_tag="VHM69_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741078.1"
                     /translation="MTVQADIESSMVAAAPFVGADSRVIELPEEVDVCYRLIEPGDAH
                     ALQRFHGRLSQRSVYLRFFLAKPARTGSSSWDTCRHREGHQYSHGDPSPENATMLNQL
                     RDLGLPDRLRYEGDVEYVDIDLSPSEVVPDHPAGTSSGPLG"
     gene            3971..5080
                     /locus_tag="VHM69_11585"
     CDS             3971..5080
                     /locus_tag="VHM69_11585"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX2741079.1"
                     /translation="MTLEMATQEYDMVFVGGGLAALLLLQELRTALPDRVAVVDPSPL
                     IGRTSVHWSYWSHEQTPYDRFAVGVWRKARVADMPPEPIAPFALRLVRSTDVFSHVDE
                     SLRSTSVQWLQTTARSITSRSDGLYEIATDAGTMHAGWVFDSAIDLDPVFPSPHEPRA
                     VESGTGIRVKSDRPVFDAETATIFDPIDERSFAYLLPLSPFEALLESASFGPVAKEED
                     QASLLRYLRMRHPEVRFQVTHVEHGAIPLGFAPPRSTGPRHILLGAKRGLIKPSAGYG
                     IVRIARESEHLARLWRENMPIPPSGRSQWQWRILDKGFLQLAAQDHRLPLALLHRVMG
                     AIPLAQSLRFIDEELPLWELRPLIRSAAPVVLGGR"
BASE COUNT          974 a         1678 c         1592 g          953 t
ORIGIN      
        1 ccgacggccc agaatcagat aaacatcgag aacgacctga gggattactt caacgagaac
       61 ctcgacaaag acgaagaggc gttgcgtata gacgccgaga gcatcgtacg cgcctacgac
      121 ccctgcatgt cgtgcgcgac caactccctg aagatagact ggatacgaag gtagtcgact
      181 tctccttctt gtggtatccc gattatggtg acctcctcca gtttgcccag tttcttcaag
      241 tacctcagtt gaaaagccag gtcgaagtcg tgcaccgatc cgcgagggga caggatcaca
      301 ccatcaaccc cttcgatcaa cgccacgtcc cgtatcccct ggaccgtatc gaggatcacg
      361 acgcgtcgct cgtccgcaaa aggcacgtct tcattgggac ttatgaagac aaacgagatg
      421 ccttcgatcg caccttcaag ctctcttgca gcttcgatcg cccttctgtc ttccgccacg
      481 tgttcgttgc cgaaaaaata gactctcatc tctttgattc tagcagggag aattgggctt
      541 ctatgaggtc tcccttatcc cttctgcctg tctctcacgc ctgcgcaggg tccagcgccg
      601 gatttcgtcg gtgccccaca cgacgaatgg gaagatggcg aggagggcga cttcgcgcaa
      661 gccaagtgcg tgagtgccga agactgcttg cagtggtgga aggtagatga ccatagcggc
      721 gaagaccagc tcgaagagga tcccccagag gagcagcctg ttggagaaga ccccgatctc
      781 gcgcagcgag gcgtggcttg tgcgagtggc gaacgcagtg ccaacctgac aggctgtaat
      841 acccgcgaag gtcatggtcg tggccgcgag gtaggccccg tgcagatggc tgccctgacc
      901 cgtcgcctcg cctggcgacc agcctgccga gtagaggacg aagaagaacc cgccggtcac
      961 gagcgcagcc tccacgagcc ccagtgtgcc ccacgcccgc gccaacatcg ggcggtctat
     1021 gatgcccctg tcgcgcgggc gcggaggtct ttgcatgacg cctggttcgg ctggctcgcg
     1081 tcccagggcg agagccggca gggtctccgt accgaggtct atggccagga tctgcataac
     1141 ggtaagcgga agcggtatcg cgccgccgga gagggcgtag atcaagaacg gcacaacctc
     1201 gggcgtggcg tgggcgaaga tgtaggtaac gaacttgcgg atgttgtcgt agacgacgcg
     1261 gccctcctcc acggccgcca cgatcgaggc gaaagaatcg tcggtgagca ccatagttgc
     1321 agactcgcgc gccacctccg tgcccgaagc ccccatcgcc acgcctatgt ccgcgcgcct
     1381 caaggctggc gcgtcattca cgccgtcgcc ggtcatcgcc accgtgtgcc ctgaggtcct
     1441 gagggcgtca acgacgtgca gcttggtctc ggggttgctg cgcgccacga tcagctccgg
     1501 cgtgtcgcgc aggagggtgt cgagctcgtg ctggtgcatc gcgtcgacct cggaacccga
     1561 gattatgcgg ggagagccgc gcacgatgcc gaccctgcgg gccacagcct cagctgtcag
     1621 gccgtggtcg ccggtaacga cgatgatcct gataccagca ttcaaacaac gggataccgc
     1681 atcggcgaca tctggtcgcg gcgggtcctc aagggctgcg agcccaagaa acgtgagctt
     1741 ggattcggcg tcgtcgcggt cttcgacggg ctccgggctc gagaccttct tctccgcaaa
     1801 gccgaggacg cgcagccctt cgcccgcgta gcgctcgaaa gccacatgca cgttcctgcg
     1861 gtcttcgtcc gtcagtaact tgtcgccgtc ggctccgcga accgacgtgc agcgctcgag
     1921 cagctccagt ggcgctccct tcacgtgata ccacaggctc ccgtccggct cctcgtctgc
     1981 cgtggtcatg cgcttgactc tggcgtcgaa gtggtagagc cgacggcggc gctttccgcg
     2041 ctcctcttgg gcgcgctcga cgtcctcgcc gagtagcgcc gcagcagcaa gcaaggcgct
     2101 ctcactcggg tctccagtgc gttcccaacc atcattcgcg cgtcgcaagc ctgcgttcga
     2161 gcaccgcaca gcggtgcgca gaagccctga tagcggttcg ctgccaaccg atccgtcgtg
     2221 cttgggatcc atttcctttc catcggccca gaaacgggag aggatcattc gtccttcagt
     2281 gagggtgcct gtcttgtccg tacagatcac atctgtcgag ccaagcgtct ccacggcggt
     2341 caggcgcttg aggagcgccc tgcggcgtgc catacgccgc accccgaccg cgagcgccag
     2401 agtgatcgtt ggcaagaggc cctcggggac gttggcaacg aggagaccta tggcgaagag
     2461 cagtgcgtcc gccaggggaa ggtcggccag cgcggctccc gcggtcagga agagtacgcc
     2521 tgcgccaacg gcgacgaagg agatcaggcg ggcggcgcgg ttcacctgca actgcagggg
     2581 actgacctca accttcacgc gctgggtgag ggtggcgatc cgcccgaatt gcgtcgccat
     2641 ccctgtcgcg tagacgacgg cctcggcctc gccaccggtc acgagcgtac ccccgaaaag
     2701 caggtcgtcg gattctagag gtgatgacgc aggacggaag cgcacagcgc cgcgcgccac
     2761 aggctgtgac tcgcccgtca acgccgacat gtcgacctcg accgaaccat caattagacg
     2821 ggcgtcagcc gagagcctgt cgccctcgga cagcagcagc acgtctcccg gtacgagagt
     2881 agtggcctcg acctcgacag ctcctcccgc gcggcgcacg cgcgcgtgcg gaggcaagta
     2941 ctgcttgagc gcctccgtcg ccttctcggc ctggagctcc tggaagaaag cgaagacggc
     3001 gttgaggact atgacaccga cgattgcgac cgagagggag agagtaccac cgacgaaggc
     3061 cagggctgcg gccgcccaca ggaggagcgc gagcgggtga gtgaactggc gtgcgaactc
     3121 gctgaggcgg ctgacgctct tgcggcgccg gatctcgttc ggaccgtgct gctcgagtct
     3181 tcgggccgcc tccctctcgg agagaccctg ccgccttgtc ccgagatggc cgagtaggag
     3241 gtcgaggcgc tcttcagggt ccagagagac cctggcatcc tcgctcatat tctggcgccc
     3301 tgtggagaag agaccatcag cccagcggcc cgctgctggt gcccgccgga tgatcgggga
     3361 cgacctccga aggtgagagg tctatatcga cgtactcgac gtcgccctcg tagcgtaatc
     3421 tgtcaggcag gccaaggtct ctgagctggt tgagcatcgt cgcgttctcg ggagaaggat
     3481 ccccgtgaga gtactgatgc ccctctctat ggcggcatgt atcccacgaa ctcgaacctg
     3541 tcctcgcggg ctttgcgagg aagaaccgca aatacactga acgctggctc aggcggccgt
     3601 gaaagcgctg cagggcgtgc gcgtcccccg gctctatcag ccgatagcaa acgtcgacct
     3661 cttcgggaag ctctatcacc ctactatctg cacccacgaa cggtgcagcg gcgaccatag
     3721 aactctctat atccgcctgc actgtcaaag gtatctcctg acatacatta ggggttctat
     3781 tggagaacag cttatcctgc tggcagggcg catcactgag cagtttgcgc catccacatg
     3841 gccaatattg cccaagtgct ttctcgaaca ccacgggccg aggaaagagt ggcgtcccgc
     3901 cgctgacggt tccttcggct gtatgaagag acggcgacag agcagtagtt tcgaggcgaa
     3961 gaagcgtaga atgaccctgg agatggctac tcaagagtat gacatggtct tcgttggcgg
     4021 cgggctggct gccttgctgc ttctgcaaga gttgcggacg gcgttgcctg acagggtcgc
     4081 ggtcgtggat ccttccccgc tcatcgggcg cacctccgtt cactggagct actggagcca
     4141 cgaacagacc ccctatgacc ggttcgcggt tggcgtctgg cggaaggcca gggtggcgga
     4201 catgccgccc gagcccatcg ccccgtttgc cttgcggctg gtgcgttcca ccgacgtttt
     4261 ctcgcacgtc gatgagagcc tgagatccac ttccgtccag tggttgcaga ccacggccag
     4321 atcgatcacg agccggtcgg atggtctcta cgagatagcc accgacgccg gcaccatgca
     4381 cgccggctgg gtgttcgaca gcgccatcga cctcgacccc gtcttccctt cgccacatga
     4441 accgcgggcg gtagagagtg gcacgggcat ccgtgtaaag tcagaccgtc ctgtcttcga
     4501 cgctgagacc gccactatct tcgatcctat cgacgaacgc tcattcgcct accttctacc
     4561 gttgagcccc ttcgaggcgc tactcgaatc cgcctcattc ggtccggttg ccaaggagga
     4621 agatcaggcg tccttgttac ggtacctccg aatgcgccac ccggaagtcc ggtttcaggt
     4681 cacccacgta gagcacggcg ccatcccact cgggttcgcg cctcccagaa gcaccggccc
     4741 tcggcacatc ctgctcggcg cgaagcgcgg actgatcaaa cccagcgccg gctacggtat
     4801 cgttcgcatt gccagggaga gtgagcacct cgcccgcctg tggcgagaga acatgccgat
     4861 tccgccaagc gggcggagcc agtggcagtg gcgtatcctc gacaagggat tcctgcagct
     4921 cgccgcccag gatcaccgtc tgccgctagc gctcttgcac cgcgtcatgg gcgccatacc
     4981 tctcgcccaa tcgcttcgct ttatcgacga agagcttcca ctttgggaac tccgccccct
     5041 tatcaggtcc gccgctccgg ttgtgctcgg cggaaggtag caggctcctg gccgtatgcg
     5101 ccgttcgcct cgtctcccac cgcgtccgag gagcccgggc gagatggtcg attcacgcca
     5161 acagaatgag tcattatgat caagcgcatt ccggcct
//
LOCUS       DATAIR010000480         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_245021, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000480 DATAIR010000000
VERSION     DATAIR010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245021"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VHM69_11590"
     CDS             complement(<1..352)
                     /locus_tag="VHM69_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2741080.1"
                     /translation="MTFEPLGRSLSDFTVELDVYSGPYEWLLALILKDELEIFEVPLR
                     ELVESYLGSRKPDAANGLERDTDFAGSAAALVLLKSRTLSPAIDAESAGGEEEPLSPE
                     ELADRLTEYLKIRRA"
     gene            complement(349..1482)
                     /gene="ald"
                     /locus_tag="VHM69_11595"
     CDS             complement(349..1482)
                     /gene="ald"
                     /locus_tag="VHM69_11595"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2741081.1"
                     /translation="MRIGVPKERAPGETRVALAPHAVYELTRAGHRVYVEMGAGEASS
                     IADEQYEEAGGRLVEDPARVYGDSEIVVKVYGPLEEEYEYLREGLILIAFLSLPVDLK
                     LMQALIDSRCIAFAMETIRDERGRLPVLTPMSEIAGRLTPQIGAHYLQRPFGGRGILL
                     GGSTGVRPGRVVILGCGIVGAGAARMSSGLGASVVIIDRDLDRLRRLEETRLPNVTTI
                     SASRLGIQDAVLRSDLLIGAVQIVGARTPALVDRETVSGMKEGSVIVDVDVDYGGCVE
                     TSRPTTLADPTFVEYGVTHYCVKNVPAAVPVTATRALSNALLPLMRRLAGVGLSDALG
                     ADPGLAQGVVVAEGRVVDAVLARATGSEHHQLSSVLPLHSEAR"
BASE COUNT          297 a          539 c          513 g          277 t
ORIGIN      
        1 ctgcgcgccg gatcttcagg tattcagtca gcctgtcggc caactcttca ggcgagaggg
       61 gctcttcctc gccgcctgcc gactcggcgt cgatcgcggg tgatagcgta cggctcttca
      121 gaaggacgag ggcggccgcc gagcccgcga agtccgtatc acgctccagg ccgttcgccg
      181 catcgggctt tcttgagcca aggtaggact cgaccagctc gcgtagcggt acctcgaaga
      241 tctcgagctc gtccttcagg atcagggcga ggagccactc gtagggcccc gagtacacgt
      301 ctagttcgac ggtaaagtca gagagcgagc gcccgagggg ctcgaaggtc atcgggcctc
      361 cgagtggagc ggcaagacgg aggagagctg gtggtgctcg gatcccgtag cgcgggctag
      421 gacggcgtcc acgacccgac cctcggcgac gacgacaccc tgtgcgagcc ctgggtcagc
      481 cccgagcgca tcggagaggc caaccccggc aagcctgcgc atgagcggga ggagcgcgtt
      541 gctcagagcc ctcgtggccg tgacaggcac ggccgctgga acgttcttca cacagtaatg
      601 ggtaaccccg tattcgacga aggtggggtc ggcgagggtg gtcggccggg acgtctccac
      661 gcagccccca tagtcaacgt caacgtcaac gatcacgctc ccctccttca tccctgagac
      721 cgtttcgcgg tcgacgaggg ccggcgtgcg tgcgccgact atctggacgg cgcctatcag
      781 gaggtcggag cgcaaaacgg cgtcctggat ccccagtcgg ctcgccgaga tcgtcgtcac
      841 gttcggcagg cgggtttctt cgaggcgtct gagccggtcg aggtctcggt ctatgatgac
      901 gaccgaggct ccaaggccgc tggacatcct ggccgcgcct gctccaacga taccgcaacc
      961 caggatcacc acgcgcccgg gcctcacgcc cgtcgagccc ccgagcagga tgccgcgtcc
     1021 cccgaacggg cgttgcaggt agtgtgcgcc tatctggggt gtgaggcgac cagcgatctc
     1081 gctcatcggc gtgagcacgg gcagccttcc ccgctcgtcg cggatcgtct ccatcgcgaa
     1141 agcgatacac ctggagtcaa tgagtgcctg catcagtttc agatcgacag gaagggagag
     1201 gaaggcgatc aagatcaaac cctccctcag atactcgtac tcttcttcta agggaccata
     1261 cactttgacg acgatctcag agtcaccgta gacgcgcgca gggtcctcga cgaggcgccc
     1321 gccagcttcc tcgtactgct cgtcggcgat gctcgacgcc tcacccgccc ccatctcgac
     1381 ataaaccctg tgacccgcgc gggtcaactc gtaaacggca tgcggtgcaa gcgcgacgcg
     1441 cgtctcgccg ggcgcccgct ctttgggcac cccgatcctc atcgtccggc tccaggagga
     1501 gtcggcgcaa gatcctggac gtggcccccg taaggatatt gcggtctcat gccttcaaag
     1561 agtaacctgg atcgccgcgg cgatccagca ccacgtcctg gaattctcag gacatttccc
     1621 aggaag
//
LOCUS       DATAIR010000481         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_400305, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000481 DATAIR010000000
VERSION     DATAIR010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400305"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VHM69_11600"
     CDS             <1..922
                     /locus_tag="VHM69_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2741082.1"
                     /translation="VAIVPSVSYGIAVAAANIPVREGQKIIILEDQFPSNVYAWRELA
                     AHREAKLVTVPRPEDHDWTRAIMEELDTDTAVVAVPNCHWTDGSLVDLARVGAGAREA
                     GAALVVDGIQSLGAHPFDVSEVEPDFLIASTYKWLLGPYGVGFMHVGEKYREGRPIEH
                     NWINRRGSEAFSQLVDYEDAFHPGARRYDVGERSNFVLLPMAAVALRQLLDWGVGNVS
                     ETIGKLTDLIEERAGEHGMETIPKERRARHMIGLMLGPNAPDDLATRLTAHDVFVSVR
                     GGSMRVSPHLYNTESDVDRLFEAISTSLQG"
     gene            1015..1848
                     /locus_tag="VHM69_11605"
     CDS             1015..1848
                     /locus_tag="VHM69_11605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2741083.1"
                     /translation="MGIGPKLAEIGRTADEAGFASLWVMDHFFQIGAVGELDEPMLEG
                     YSALSYLAAVTERAKLGTLVTGVIYRYPGLLVKTATTLDVLSGGRAYLGIGAGWNERE
                     SRGLGFPFPSTRERFERLEEALQIAHQMWSGEVRSYEGEHYQLAEPLNSPQALSEPHP
                     PVMIGGMGEKRTLRLVAKYADACNLFAYGGPDLIRHKLDVLRGHCEDVGRDYEEIERT
                     ALATANLAEGGMTADQVIGLCNELNEAGIQHVIFNMPNVHEIKPLVTFGEEVIPAVTG
                     L"
     gene            complement(1963..2205)
                     /locus_tag="VHM69_11610"
     CDS             complement(1963..2205)
                     /locus_tag="VHM69_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741084.1"
                     /translation="MERDEARNVEGMRGDAPQITGFLGRGKFVTVSARTGIDLRGYVC
                     DQSESGLLLDARDQGGDPTGYEFLPWSSIERVSAGQ"
     gene            complement(2525..3151)
                     /locus_tag="VHM69_11615"
     CDS             complement(2525..3151)
                     /locus_tag="VHM69_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741085.1"
                     /translation="MFRSPSVRRLILLGPSLALAVLMLFHPSPYDDVAGELLPIAGWW
                     TLLHTVQFFLFAFVGASVWMLVDGLRGAAAVVARVAAVVFILFYDIGDAVAGIATGIL
                     AGDAAGGALPEDAAVTAIDAIFSNLTKDVFFGIGISAWILALVASAVALWRTGAPRLP
                     VVLLALPAFLLTFDHAFPFGALTFATFFFIALWLEMPRRDRIRPRTVA"
     gene            complement(3187..3741)
                     /locus_tag="VHM69_11620"
     CDS             complement(3187..3741)
                     /locus_tag="VHM69_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741086.1"
                     /translation="MDSTIWDTGAWVCEAVLDVTGETLDMAEVTTWTHVLDTYGERIT
                     TTIFDRVFDPARIGEREPYAGASKVLRRLQVERGLEVHFVTHNDPEVMTSHLEPWLTT
                     HFGSRVGLTVTTGDKLTILEEMGAFGLIDDRPDTIRRAADAGLWAATKIQPWNRDLVE
                     RRDDVHGFSEWWEVPDLLPSLQGG"
BASE COUNT          776 a         1213 c         1217 g          651 t
ORIGIN      
        1 cgtggcgata gtcccctccg tcagctacgg gatcgccgtc gccgccgcga acattcccgt
       61 gcgagagggc cagaagataa ttatcctgga agaccagttc ccctccaacg tctacgcgtg
      121 gcgcgagctg gcggcgcaca gagaggcgaa gctcgttacc gtaccccgtc cagaagatca
      181 cgactggacc agggctatta tggaggagct cgataccgac accgccgtcg tcgccgtccc
      241 gaactgccac tggaccgacg gctcgctcgt tgacctcgcg cgcgtcggag cgggagcgcg
      301 ggaggctgga gcggcgctcg tggtcgacgg aatacagtcg ctaggggcgc accctttcga
      361 cgtttccgag gtcgaacccg acttcctcat cgcctcgacc tacaagtggc tgctcgggcc
      421 ctacggcgtc gggttcatgc acgtcgggga gaaatatcgg gaggggcggc ccatagagca
      481 caactggatc aaccgccgcg gcagcgaggc tttctcgcag ctggtggact acgaggacgc
      541 tttccaccca ggagcgcggc gctacgacgt cggggagcgg agcaacttcg tcctgctccc
      601 gatggccgcc gtggcgctgc gccagctcct tgattggggt gtcgggaacg tctccgagac
      661 tatcggcaag ttgactgacc tcatagagga aagggccgga gaacacggta tggagacgat
      721 accgaaagag aggcgggcgc ggcacatgat cgggctcatg ctcggtccta acgcccctga
      781 tgacctcgcc accaggctca cggcgcacga tgtcttcgtc agcgttcgcg gagggagcat
      841 gcgcgtctcc ccccacctct acaacaccga aagcgacgta gaccgtctct tcgaggctat
      901 ctccacgtcc ctccaggggt agaatctgct ggtcgagagt gcatcaaccc gagagaggca
      961 ggagatgtgc gcataggact gcaagttcca agtttcacct ggccggaagg cgatgtgggg
     1021 ataggtccca agctggccga gatcggcagg accgccgacg aagcaggttt cgcgagcctg
     1081 tgggtgatgg accacttctt ccagataggg gccgttggcg agctggatga gccgatgctc
     1141 gaagggtaca gcgccctgag ctacctggcc gccgtcaccg agagggcgaa actcgggacc
     1201 ctggtgacgg gtgtcatcta ccgctacccc ggtctcctcg tcaagacggc aaccacgctc
     1261 gacgtgctct cggggggaag ggcttacctc ggtatcgggg ccggttggaa cgagcgcgaa
     1321 tcgcggggac ttggctttcc ttttccctcc accagagagc gcttcgagcg actcgaagaa
     1381 gcgctccaga tcgcgcacca gatgtggtcc ggtgaggtca ggtcttacga gggcgagcat
     1441 taccaactgg ccgagccgct caacagcccc caggctctca gcgagccgca cccgccggtg
     1501 atgatcggcg ggatgggcga gaagagaacg ttgcggctcg tcgccaagta tgccgacgcc
     1561 tgcaacctgt tcgcgtacgg aggtcctgat ctgatccgcc acaagctcga cgtccttcgg
     1621 ggtcattgcg aggacgttgg aagggattat gaggagatcg aacgcacagc cctcgccacc
     1681 gccaacctcg cggaaggcgg catgaccgcc gaccaggtga tagggctgtg caacgagctg
     1741 aacgaggcag gcatacagca cgtcatcttc aacatgccga acgtgcacga gataaagccg
     1801 ctcgtgacgt tcggcgaaga ggtcatccct gccgttaccg gattgtagtt cccctccgcc
     1861 agggtgccta tattagaccc atcgagccgc ctgtatagcg catgaactcg ggactcaccc
     1921 cgctcagcag cagatggtaa ggtcatcact gctccgatac ccttactggc cggcgctcac
     1981 ccgctcgatg ctcgaccagg gaaggaactc atagccggtc gggtccccgc cctggtctct
     2041 ggcgtcgagt agaagcccgc tctcgctctg gtcgcagacg tagcccctca ggtcgatgcc
     2101 ggtcctggcc gagaccgtaa cgaacttccc gcggcccaag aaccctgtta tctgcggcgc
     2161 gtccccccgc ataccctcca cgttcctggc ctcatcgcgt tccaaagcgc ctcctccgtg
     2221 tccaatgcga tgtgcgtaat catgacttcc gcacatcgcg cttcggccct cgctatcagt
     2281 gtttgagatc ttgcctctgt agcagatttc cctcttcctg ggtccatctt ttcgagagac
     2341 gctctagcgt tcagcaccag acaggttaca ttaacttgcc tcgtccgttt catcggcgca
     2401 tcgataagcc gaaaaggtga cggagagcat ccttgcggcg agtacgcccg acggggtcaa
     2461 cggcgactgc gagcgggtga cgagaaggta gtcgaacaga acacggggag cccggctctg
     2521 cagttcaggc gacggttcgg gggcgtatgc ggtcccgcct cggcatctcc agccagaggg
     2581 cgatgaagaa gaaagtagcg aaggtgaggg caccgaaagg gaaggcgtga tcgaaggtga
     2641 gcaagaacgc cggtagggcc agcaagacga cgggcagacg gggcgctccg gtgcgccaca
     2701 gcgcgaccgc agacgcgacg agggctaaga tccaggccga tattccaata ccgaagaaca
     2761 cgtccttcgt gaggttggag aagatcgcat ctatcgcggt gacggcggcg tcctcgggaa
     2821 gggcgccgcc cgctgcatcg ccggcgagaa tgcctgtggc gatacccgcc acggcgtccc
     2881 cgatatcgta gaacaggatg aagaccaccg cggccacccg cgcgactacg gcggcggccc
     2941 cgcgcaagcc atcgaccagc atccacaccg acgcgcccac gaacgcgaac aggaagaact
     3001 gcaccgtgtg cagaagggtc caccatccgg cgatgggtaa aagctcgccg gctacgtcgt
     3061 cgtaaggcga cggatggaag agcatcagca cagccagagc gagggagggg ccgagcagga
     3121 tcaggcgccg cacggatgga gagcggaaca cggcatcacc tccagaagcg gacgtcgtgc
     3181 atggatttaa ccaccttgga gagacggcag gagatccggg acctcccacc actctgagaa
     3241 accgtgcacg tcgtccctcc gctcgaccag gtcgcggttc cagggttgaa tcttggtcgc
     3301 cgcccacagg cccgcatcgg cggcccgccg gatggtatcc ggcctgtcgt ctatgaggcc
     3361 gaaggcgccc atctcctcca ggatggtgag cttgtccccc gtcgtaaccg tgagaccaac
     3421 gcgtgagccg aagtgcgtcg tgagccaggg ctccagatga gaggtcatga cctcagggtc
     3481 gttgtgggtt acgaaatgaa cctctagtcc acgctccacc tgtagcctac gcagtacctt
     3541 cgaggcgccc gcataaggtt cccgctcgcc gatgcgggca gggtcgaaga cacggtcgaa
     3601 gatcgtggtc gtgatcctct ctccataagt atccaggacg tgggtccagg tcgtcacctc
     3661 ggccatatca agcgtctcgc ccgtgacatc gaggaccgcc tcacagaccc aggcgcccgt
     3721 atcccagatc gtagaatcca catcacagca gagtgtcggt ttcagggtta gatcagctcc
     3781 tcgacgggga tcgcctcggt gaggccggac gccgggtcca cgacagcgta ggctgccccc
     3841 gcctgtcgcg ctatgag
//
LOCUS       DATAIR010000482         7889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_400522, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000482 DATAIR010000000
VERSION     DATAIR010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400522"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VHM69_11625"
     CDS             <1..1248
                     /locus_tag="VHM69_11625"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEX2741087.1"
                     /translation="MGFFKPVDYWKWAKREDVVSDDSYPDPSDPEAHIGAAMSRDLMR
                     SLGGGDPWVLMEQTTVRVNWRDRNVPKRPNEMRLWSYGAVARGAEGIMFFQWRQSRAG
                     AEKFHSAMVPHVRPEDSRSWREVSRLGAELGKLDGLIGARGEARAAILLDWESWWALE
                     LDSKPSSAVRMLDGVYSFYKPLFEANVPVDFAHPGSDLSSYRFVIAPNLYLVNDESVE
                     NVRRFVADGGTLLMSFFSGIVDARDHIRLGGYPAPFRELLGLRIEDFIPMDAGERNRL
                     DDGTYTCELWADLIHLEGAEALASYSDGFYTGTPAVTRNVFGDGVAYYLGTRPEERYV
                     KSLFERACEEAGVRPTVEAPPGVDAVRRKTVDASFLFVLNHNEEAVEVRLPSPGRDLL
                     AGTEHDSSLVLEPLGVAILHQTQ"
     gene            1509..2831
                     /locus_tag="VHM69_11630"
     CDS             1509..2831
                     /locus_tag="VHM69_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2741088.1"
                     /translation="MKRTLTRRGFLKVGGGALASAYVLGLAGCGGGGEQDGTSKSLEF
                     WGFDEGRVNFAKAGYKSKAFKDKYSDVEITFRILPYEQMHDKLLTALVSGQGAPDIAD
                     IEISRFGQFIKGDRVPLLGLEDRIGDAINDIYKPAAVDPWSWKGEIYGVGNELNTVVL
                     AYRRDILEDAGIQLPFATWDDVIAAGQEISTGDRKMFAIHDLAFGDHYMMMQHAGTAY
                     FDENGNYNGDDPRSVEAMQFLHDLVYEHGVADIAPAEANDNWAPPQYMASYRAERFIA
                     TFGPPWHLSFFIDQVPDQAGKWDVQELPEGLGESRPTANFGGTGMAITEQSGNPEASW
                     ALIEAANLTKEGVLFDFNARTAYPAYKPAYEDPALAQPNEYFGGTKIGQLYSDIADDL
                     PTFRQSPVWPDTTEALQRDVITPVMNDKADAKAALTELRGTVEELQKK"
     gene            2877..3836
                     /locus_tag="VHM69_11635"
     CDS             2877..3836
                     /locus_tag="VHM69_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2741089.1"
                     /translation="MQPQVDEAGQGGARKTAPGGATAKRRWSGGRRSTVAPYLFLLPF
                     FAIYCTFLLYPTFSALWLSFQEAVALDTPTFIGLDNYVRLVQDERYLHALRNTTVYAL
                     ASVFILSPLALAVALAIRSFLVPSANLKSFYRISLFLPFITSFVVIALMFSLIFNKDF
                     GLLNSFLRSIGLPELNWLRSEQLAMLSIVLVGIWTYLGVNSLYFLAGLQNIPHELNEA
                     AAIDGANRRKTFFYVTLPLLRPTILFVVIQATIFSYQLFEIPFLLTNGGPSDATLFLS
                     QYLYEVGFSQFDRGYAAAIGYSMALISIFLTVIQLLLFRRFSR"
     gene            3833..4672
                     /locus_tag="VHM69_11640"
     CDS             3833..4672
                     /locus_tag="VHM69_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2741090.1"
                     /translation="MITGNKAATRMWQVIGNGVLLLVIFLTIMPYVYMVSASFKPGEE
                     LFSIPVQIFPDSLYWGNYDLLFGKTNFVRWFFNSVFIGVSRMILAAVISLMAGYAFAK
                     FDFRFKNVLFIMLLATLTLPIYVLIVPLYSMMVTFGWTDSYIALILPLAAQAIGVFLS
                     RQYLLSIPDEILDSARVDGAGEWGIFWRIIVPLSQPVIAVMAILFFTASWNDYIWPLV
                     VLTQDQMFTVSLGLPTLVGPYSQEYGAVMAGSFLSTVPIMIIFLIMQRRFIEGITQGA
                     VKG"
     gene            4701..6110
                     /locus_tag="VHM69_11645"
     CDS             4701..6110
                     /locus_tag="VHM69_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX2741091.1"
                     /translation="MSNGRAPLRVQGNRIVNGEGKNVVLRGFGLGGWMNMENFITGYP
                     ANEEAQREAVRAVLGEERNRYFFDRFLEYFFSEGDARSISSLGLNLLRLPVNYRHFED
                     DMRPFEIIEDGFRHLDRVIELCGAHGIYTIIDLHALPGYQNQHWHSDNPTHKALLWRH
                     KYFQDRTVNIWEHVAERYRGNPWVAGYNPINEPADPSEQLIMPFYRRLYEAILAVDPD
                     HIIFLEGNRYSVDFHMFGEPWEGVVYTNHDYALPGFIDGGPYPGVSRGEYVDKEVLEE
                     TFLQRSEYMLQHDVPIWVGEFGPVYPPDDPDSHAMRRELLRDQLEIYARHGVNWAIWT
                     YKDIGLQGVQYAAPDSPWVTRIRPILEKKARLGVDAWGGTDAKIRQVMEPLERLFAQE
                     YPDYKPFPFGTQREIELLVRNILLAEPMLPEYGELFRGMDEDGIDAMMRSFSFENCVR
                     REELASILSEHAVQGGAQG"
     gene            6142..7503
                     /locus_tag="VHM69_11650"
     CDS             6142..7503
                     /locus_tag="VHM69_11650"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX2741092.1"
                     /translation="MNFPEGFLWGTATASYQVEGAVNEDGRGTSIWDTFSHTPGKVRH
                     GDTGDIACDQYHRLDEDLDIMSELGMQTYRFSVAWPRIQPDGSGPANQKGLDYYRRLV
                     EGLRQRSIEPMLTLYHWDLPQALEDLGGWTNRDTSERFSEYAGIVYEALSDSVGSWIT
                     LNEPWVAAWMGYGLGVHAPGHKDSAKALAATHHLLLGHGLAMERMRSAGDANQLGVTL
                     NLHPARPSRDRDADREAARRVDGQANRLYLDPLFRGEYPQDIFSYYEERGADLSFVHD
                     ADLEKISVPIDFLGINYYFRNTVRDAQEEDLPDMPFADLGARPVIPHDAEKTAMGWPV
                     EPEGLTEILVRIKEDYADVPMYVTENGRAVHDYIDPEGGVDDEERVSYLDAHFRAAHD
                     AMEQGVDLRGYMVWSFLDNFEWAEGYSKRFGIVFVEYGTQRRVPKSSARWYSEVIRRN
                     GLE"
     gene            7527..>7889
                     /locus_tag="VHM69_11655"
     CDS             7527..>7889
                     /locus_tag="VHM69_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2741093.1"
                     /translation="MEYRDLGETGMRVSEISLGTWAFGGDWGTVGEDDAYAALNRAVD
                     LGVNFLDTADVYGDGRSEKLIGRLLKDRPNDEILVGTKGGRRLDPHTAEGYNYDNLSA
                     FVERSLENLGVEALDLLQL"
BASE COUNT         1526 a         2439 c         2456 g         1468 t
ORIGIN      
        1 atgggcttct tcaagcccgt ggattactgg aagtgggcga agcgcgagga cgtcgtctcc
       61 gacgactcgt accccgaccc gtcggacccg gaggcccaca tcggtgccgc gatgagccgc
      121 gacctgatgc ggtcccttgg aggcggcgac ccgtgggtcc tcatggaaca gacgacggta
      181 cgcgtgaact ggcgcgatcg taacgtcccg aagcggccta acgagatgcg cctctggagc
      241 tatggggccg tcgcccgcgg ggccgagggc atcatgttct tccagtggcg gcaatccagg
      301 gctggagccg agaagttcca cagcgcgatg gtcccgcacg tgaggcccga ggactccagg
      361 agctggcgcg aggtcagcag actcggggcg gagctcggca aactggacgg gctcatcggc
      421 gcacgcgggg aggcgcgggc tgcgatcctg ctcgattggg agagctggtg ggcactggaa
      481 ctagactcca aaccctcgtc cgcagtccgt atgctcgacg gcgtctactc tttctacaag
      541 ccgctgttcg aggccaacgt ccccgtggac ttcgcccatc ccggctcgga cctctcgtcg
      601 tacagattcg tcatcgcccc gaacctctac ctcgtgaacg atgagtctgt ggagaacgtc
      661 aggcgtttcg tggcggatgg cggcacgctc ttgatgtcgt tcttctcggg tatagtggac
      721 gcgcgggatc acatccgtct cggcggctac cctgccccgt tccgtgagct gctcggcctt
      781 cgtatcgagg acttcatccc gatggatgcc ggagaaagga atcgcctcga cgacggtacc
      841 tatacatgcg agctatgggc ggatctcatc cacctcgaag gtgccgaagc gctggcttcc
      901 tactcggacg gcttctacac ggggacgccg gccgtgacgc gcaacgtttt cggagatggc
      961 gtcgcctact acctcggcac caggccggag gaacgctacg tgaagtcgct cttcgagcgg
     1021 gcgtgcgagg aggccggtgt gagaccgacg gtcgaggccc cacccggtgt cgacgccgta
     1081 cgtcgcaaaa cagtggacgc atccttcctc ttcgtgctca accataacga ggaagccgta
     1141 gaggtcaggc tgccgagtcc tggccgggat ctactcgcgg gaacggagca cgattcgagc
     1201 ctggttctcg aacccctcgg agtcgcgatc ctgcaccaga cgcaatgatg acgccgctcg
     1261 ccaagcacgg gaaatgcact tcgagcggtt tgacacaaaa ccttgccttg actatattgg
     1321 cttgcgtaga ctttcggggt tgacaggctt ggtctgggga aagacgggcg ctggattgaa
     1381 cgaggcaagg gagcattcgc cccgcacgct ggcattcgcc ggggcgcggg tgctgacgtc
     1441 atttgtgtcc aggtccccgg taaaggtggg aacctgtgga taagcgtcgt tctggtcagg
     1501 aggccgaagt gaagcggacg ttgacgaggc gcggattttt gaaggtgggt gggggagcgc
     1561 tggccagcgc ttacgtcctc gggctcgccg gatgcggagg aggaggtgag caggacggaa
     1621 cctccaagag cctggagttc tggggcttcg acgaggggcg cgtcaacttc gccaaggcgg
     1681 gctacaagtc caaagcgttc aaggacaagt acagcgacgt cgagatcacc ttcaggatac
     1741 ttccttacga gcagatgcac gacaagctcc tgacggccct cgtatcaggg cagggcgccc
     1801 ccgacatagc ggacatcgag atctcgcgtt tcgggcagtt catcaagggc gacagggtgc
     1861 cgctcctcgg tctcgaagac cgaatcggcg acgcgataaa cgacatctac aagcctgcag
     1921 cggtggaccc gtggagctgg aagggtgaga tctacggcgt cggcaacgag ctgaacaccg
     1981 tggtactggc ctaccgcagg gacattctgg aggacgctgg catccagctt ccattcgcga
     2041 cctgggacga cgtgatcgct gcaggtcagg agatctcgac gggcgaccgc aagatgttcg
     2101 ccatccacga cctcgccttc ggcgaccatt acatgatgat gcagcacgcc ggaaccgcct
     2161 acttcgatga gaacggcaac tacaacgggg acgacccgag gagcgtcgag gccatgcagt
     2221 tcctgcacga cctcgtctac gagcacgggg tcgccgacat agcccctgcc gaggccaacg
     2281 ataactgggc gcctccgcag tacatggcgt cctacagggc cgagcggttc atcgcgacgt
     2341 tcgggccccc gtggcacctc tcgttcttta tcgaccaggt ccccgaccag gcaggcaagt
     2401 gggacgtgca ggaactgcca gaaggactcg gagagagccg gccgacggcc aacttcggcg
     2461 gcacgggcat ggcgattacg gaacagtcgg gcaaccccga ggcgtcgtgg gcccttatag
     2521 aggcggccaa cctcaccaag gaaggggtcc tcttcgactt caacgcgcgg acggcgtacc
     2581 cggcctacaa gccggcctac gaggacccag cgctcgcgca gcccaacgag tacttcggcg
     2641 gcaccaagat cggccagctc tactccgaca tcgccgacga ccttccgacc ttccgccagt
     2701 cgccggtgtg gcccgacacc acagaggcgc tgcagcgcga tgtgatcaca ccggtcatga
     2761 acgacaaggc ggacgccaag gcagccctga ccgagctgag ggggaccgtc gaagagttgc
     2821 agaagaagta gccgaaggac cagtctccat cacgttactc gggaggttga gtgacgatgc
     2881 agccgcaagt tgacgaagcg ggccaggggg gagcgcgcaa gacggcgccc ggcggggcca
     2941 cagccaaacg ccggtggagc ggtggacggc gctcgacggt agccccgtac ctattcttgc
     3001 tgccgttctt cgcgatctac tgcactttcc tgctctaccc cacgttcagc gcgctgtggc
     3061 tgagctttca agaggcggtc gccctagaca cgcccacgtt catcgggctg gacaactacg
     3121 taaggctggt gcaggacgag cgctacctcc acgccttgag aaacaccacc gtctacgccc
     3181 ttgcgagcgt gttcatactc agcccgctgg cgctcgcggt cgcgctcgcg ataaggtcgt
     3241 tcctcgttcc atcagcgaac ctcaagagct tctaccgcat ctcgctgttc ttgccgttca
     3301 tcacctcgtt cgtcgtgata gcgctcatgt tcagcctcat cttcaacaag gacttcggtc
     3361 tgctcaactc cttcctccga tcgatcgggt tgcctgagct caactggctg aggtccgagc
     3421 agctcgcgat gctctccatc gtcctggtcg ggatctggac gtacctcggg gtgaactcgc
     3481 tgtacttcct cgccgggctg cagaacatcc cgcacgagct gaacgaggcg gccgccatag
     3541 acggcgcaaa ccgcaggaag acgttcttct acgtcacgct cccgttgctc aggccgacga
     3601 tactgttcgt cgtcatccag gcgactatct tcagttacca gctcttcgag ataccgttcc
     3661 tgctgaccaa cggcgggccc tctgacgcga cgctgttcct ctcgcagtac ctttacgagg
     3721 tcggtttctc gcagttcgac cgtggctacg cggcggcgat agggtattcg atggctctta
     3781 taagcatctt tctgaccgtc atacagctgc tccttttcag gaggttcagc agatgattac
     3841 gggcaacaag gcggcgaccc gtatgtggca ggtgatcggc aatggcgtat tgttgctggt
     3901 catcttcctg accatcatgc cctacgtcta catggtcagc gcctccttca aacccggcga
     3961 ggagctattc tccatccccg tacagatatt ccccgacagc ctgtactggg gcaactacga
     4021 cctcctcttc gggaagacga acttcgtccg ctggttcttt aacagcgtct tcataggagt
     4081 gtcgcgcatg atactcgcgg ccgtgatctc cctcatggcc ggctacgcct tcgcgaagtt
     4141 cgacttccgt tttaagaacg tcctcttcat catgctgctt gcgaccctga cgctcccgat
     4201 atacgtcctg atcgtgccgc tctactcgat gatggttact ttcggctgga ctgactcgta
     4261 catcgccctc atcctgccgc tcgcggccca ggccataggc gtcttcctct cgcgccagta
     4321 tctgctctcg atccccgacg agattctcga ctcggcccgc gtcgacggcg ccggggagtg
     4381 ggggatcttc tggcggataa tcgtgcccct ctcacagcct gtgatagcgg tgatggcgat
     4441 actcttcttc accgcttcct ggaacgacta catctggccg ctcgtagtgc tgacgcagga
     4501 tcagatgttc accgtctcgc tgggacttcc gaccctggtc ggcccttaca gccaggagta
     4561 cggggctgtc atggctggtt ccttcctgag cacggtgcct atcatgatca tattcctgat
     4621 catgcaacgg cggtttatcg aggggatcac ccagggcgcc gtcaagggtt agaccctgca
     4681 actctttgga aggagggagc atgtcgaacg gtagggcccc gttgcgggtg caaggcaacc
     4741 gaatcgtgaa cggcgagggg aagaacgtcg tgctgcgcgg cttcggcctc ggcggctgga
     4801 tgaacatgga gaacttcata accggctacc ccgcgaacga ggaggcccag cgcgaggccg
     4861 tccgcgctgt actcggcgag gaaaggaaca gatacttctt cgaccgcttc ctcgaatatt
     4921 tcttctctga gggcgacgcc cggtctatct cctcgctcgg gctcaacctg ctcaggctgc
     4981 ccgtgaacta ccgtcacttc gaggacgaca tgcggccatt cgagatcata gaagatggct
     5041 tcaggcacct ggacagggtt atagagctat gcggggcgca cgggatctac acgatcatcg
     5101 acttgcacgc gttgccgggc taccagaatc aacactggca ctcagacaac ccgacccaca
     5161 aggcgctcct ctggaggcat aaatacttcc aggatcgtac cgtgaacatc tgggagcacg
     5221 tagccgaacg ttacagaggc aatccttggg tggctggcta caatcctata aacgagcccg
     5281 ctgacccgag cgagcaactc atcatgccgt tctaccggcg gctctacgag gcgatcctgg
     5341 ccgtggatcc ggaccacatc atcttcctcg aaggtaaccg ctactccgtg gacttccata
     5401 tgttcggcga gccgtgggaa ggcgtcgtct acacgaacca cgactacgcc ctgcccggct
     5461 ttatagatgg tggcccgtac ccgggcgtct cgcgtggcga gtacgtggac aaggaagttc
     5521 tcgaagagac cttcctgcag cgcagcgagt acatgcttca gcacgacgtg ccgatctggg
     5581 tcggcgagtt cgggcccgtg tacccgcctg acgaccctga ctctcatgcg atgcgccgcg
     5641 aactcctgcg cgatcagctg gagatctatg cccgccacgg cgtgaactgg gcgatctgga
     5701 cgtacaagga tatagggttg caaggggtcc agtacgccgc gccggactct ccgtgggtga
     5761 cgcgcattcg ccccatactg gagaagaagg cgcgcctcgg cgtggacgcc tggggcggca
     5821 ctgacgcgaa gatccggcag gttatggaac ctctggagag actcttcgcg caggagtacc
     5881 cggactacaa gccgttcccc ttcgggactc agcgggagat cgagctgcta gtgcgcaaca
     5941 tcctgctcgc cgagccgatg ctccctgagt acggggagct cttccggggt atggacgagg
     6001 atgggataga cgcgatgatg cgctcgttct ccttcgagaa ctgtgtgagg cgcgaggagc
     6061 tcgcctccat actctccgag cacgccgtgc agggaggtgc gcaaggttga cgaccgccaa
     6121 cgggcggaag gaggcagcag tttgaacttt cccgagggct tcttgtgggg tactgcgacg
     6181 gcgtcctacc aggtcgaggg ggccgtgaac gaggacggcc gcggaacttc gatctgggac
     6241 acgttcagcc ataccccggg caaggtccgc cacggcgata cgggcgacat cgcctgcgac
     6301 cagtaccacc gtctggacga agatctcgac atcatgtccg agctgggtat gcagacctac
     6361 cgcttctcag tggcatggcc gaggatacaa cccgacggga gcggtcccgc aaaccagaag
     6421 ggcctcgact actataggcg cctcgtcgaa ggcctgaggc aacgctccat cgaaccgatg
     6481 ctcaccctct accactggga cctgcctcag gccctggagg atctgggcgg ctggacgaac
     6541 cgcgatacga gcgagcgttt ctccgagtac gctggtatcg tctacgaggc gttgtcggac
     6601 tcggttgggt cctggatcac attgaacgag ccctgggtcg cggcctggat gggatacgga
     6661 ctcggcgtcc acgcccctgg acacaaagac agcgccaagg ctctcgcggc cactcaccat
     6721 ctcctgctcg gccacgggct cgcgatggag aggatgcgct ccgcagggga tgcaaaccag
     6781 ctgggcgtca cgctgaacct gcatccggcc cgtccctcgc gcgaccgcga cgccgacagg
     6841 gaggccgcgc gccgcgtgga cgggcaggct aaccggctct acctcgatcc cctgtttcgc
     6901 ggcgagtatc cgcaagatat cttctcgtac tatgaggaac gcggtgcgga cctctccttc
     6961 gtccacgatg cagatctgga gaagatctcc gttcccatcg atttcctcgg gatcaactac
     7021 tacttccgca acaccgtccg cgacgcgcag gaagaagacc tacctgacat gcccttcgcg
     7081 gacctcggcg cacgtcccgt catcccgcac gatgctgaga agacggcgat gggctggcca
     7141 gtcgaacccg agggcctgac ggagatcctg gtcaggataa aggaagacta cgcggacgtg
     7201 cccatgtacg tgacggagaa cgggcgcgct gtccacgatt acatagaccc agaaggcggc
     7261 gtggacgacg aggagcgcgt ctcgtacctg gatgcccact tccgcgccgc gcacgatgcc
     7321 atggagcagg gcgtcgacct gcgcggctac atggtgtggt ctttcctgga caacttcgag
     7381 tgggcggaag ggtactccaa acggttcggg atcgtcttcg tggagtacgg aacgcagcgg
     7441 cgcgtcccca agtcgagcgc ccgctggtac tctgaagtca tccggcgcaa cgggcttgag
     7501 tagagcttac tagaagggac tgagctgtgg aatacagaga cctcggcgag acggggatgc
     7561 gcgtctcgga gatcagcctc ggcacctggg ccttcggggg cgactggggg acggtcgggg
     7621 aggacgacgc ctacgccgcg ctcaaccgcg ctgtagacct gggcgtcaac ttcctcgaca
     7681 cagcagacgt gtacggcgac gggcgcagtg agaagctcat aggcaggctc ctcaaggacc
     7741 gaccgaacga tgagatactg gtggggacca agggcgggcg ccgcctggat ccccatacag
     7801 ccgaaggcta taactacgac aatctatcgg ctttcgtgga acggagctta gagaacctcg
     7861 gggtggaggc cctggatctc ctgcaactc
//
LOCUS       DATAIR010000483         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_400579, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000483 DATAIR010000000
VERSION     DATAIR010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400579"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VHM69_11660"
     CDS             <1..1013
                     /locus_tag="VHM69_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2741094.1"
                     /translation="HKYINPGIYIINITIPPGTSSDPSITCGPLNQQFVVEVPNYRPD
                     TYINTPPAPSPGSTVNSKDVTFTYGSNRNYVTFECSLDGSDFSECPSQGQTYSGLSDG
                     QHQFKVRARDAGDNEDLSPATRTWTIDTTAPAAPDISNPSERSLVSAKFTVSGTAEAG
                     STVELSYGSVSRSAQTDSSGQWSIELVDLTEEPHSFKARATDAAGNVSADSETRTVIV
                     DATRPTVKRVAPAENATSIAPGANVSALFSEATMEPASIKTSFKLYKKGSTTALAATV
                     TYDPDTNRAVLNPRNNLKRGATYKAVVGAGVQDLAGNTLLDQDPNRAGVQPKVWFFTV
                     RN"
     gene            complement(1092..>1556)
                     /locus_tag="VHM69_11665"
     CDS             complement(1092..>1556)
                     /locus_tag="VHM69_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741095.1"
                     /translation="EEGLARAFRRLDMTFESPEDYLNFWFPDQGLTMDDLPPDLADYY
                     LYDLGEVDGGYQPKASRAAAEEDSPSVSETSPTAEEMSAVRCPVALVRASQGFFPGTD
                     PLISDEARDVMAQILDIREEIVLDGANHYTMLWPEYTQIWAPRVFDPPFWER"
BASE COUNT          332 a          499 c          453 g          272 t
ORIGIN      
        1 ctcataagta catcaatcct ggcatttaca tcataaacat aactatcccc cccggcacct
       61 cttcggaccc ttccataacg tgtggccccc tcaaccagca gttcgtggtt gaggtgccca
      121 attaccggcc ggatacttat atcaacaccc ctccggcccc ttctcccgga agcacggtga
      181 actcgaagga tgtcaccttc acatacggct ccaacaggaa ctatgtgact ttcgagtgca
      241 gtctcgatgg tagcgatttc tcggagtgtc cctcgcaggg acaaacctac tcaggtctta
      301 gtgatgggca gcatcaattc aaggtaaggg cgagagacgc cggtgacaat gaagacctct
      361 ccccagcgac acgcacttgg acaatagaca ccacggcacc cgccgccccg gatataagca
      421 acccgtctga gcgcagcctc gtgagcgcca aattcaccgt ctcgggcacc gcagaggcgg
      481 gcagcaccgt cgaactctcc tacgggtcgg tctccaggag tgctcaaacg gactcctccg
      541 gacagtggag catagagctc gtagatctca ccgaagagcc gcactccttc aaggccaggg
      601 ctacagacgc cgcaggcaac gtctccgccg actccgaaac ccgtaccgtg atagtggacg
      661 ccaccaggcc cacggtgaag cgggtggcgc ccgcggagaa tgccacgagc atagcccctg
      721 gcgccaatgt ctcggccctg ttctcggagg cgacgatgga gcctgcctct ataaaaacta
      781 gcttcaagct ctacaaaaag ggctctacca cggcgctggc cgccaccgtc acctacgatc
      841 ccgataccaa cagggcggta ctgaacccca ggaacaacct caagcgtggc gcgacctaca
      901 aggctgtggt cggagccggc gtgcaggacc tcgcgggcaa caccctgttg gaccaggatc
      961 cgaaccgcgc gggcgtccag ccgaaggtat ggttcttcac ggtgaggaac tagacgacat
     1021 agaagcaagt gcgaacctga tctggagggc cggagggtag caactccggc cctctttcat
     1081 gccttcgtag ctcagcgctc ccagaaaggg ggatcgaaga ccctgggagc ccagatctgt
     1141 gtgtactcgg gccacagcat cgtgtaatgg ttcgcgccat cgagcacgat ctcctcgcgg
     1201 atgtccagga tctgtgccat cacgtcgcgg gcctcgtcgg agatgagagg gtcggtgccc
     1261 gggaagaagc cttgcgaagc cctgacgagc gcgacgggac agcgcacggc gctcatctct
     1321 tccgcggtcg ggctggtctc cgagacggag gggctgtcct cttccgcagc cgcgcgggag
     1381 gctttgggct ggtagccgcc atcgacctcc ccgaggtcgt agaggtagta gtccgcgaga
     1441 tcgggcggca ggtcgtccat cgtcagcccc tgatccggga accagaaatt caggtagtcc
     1501 tccgggctct cgaaggtcat gtcgaggcgt ctgaaggccc gtgcgaggcc ctcctc
//
LOCUS       DATAIR010000484         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_60229, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000484 DATAIR010000000
VERSION     DATAIR010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60229"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..574)
                     /locus_tag="VHM69_11670"
     CDS             complement(47..574)
                     /locus_tag="VHM69_11670"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2741096.1"
                     /translation="MTFPTVVLAEYDPGWPALFESEKERILDAIGPRVLAIEHVGSTA
                     VPEMTAKPIIDVMVGTRNVAATADACTASLVNIGYAYVPLPAILDRRFFRRGAWLLEC
                     MYHLHMVELGGETWHRYTLFRDVLRAYPDVARSYQELKQQLAVEYRFDRSAYTAAKNW
                     FVEGVIARAENEFKS"
     gene            complement(649..2127)
                     /locus_tag="VHM69_11675"
     CDS             complement(649..2127)
                     /locus_tag="VHM69_11675"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX2741097.1"
                     /translation="MVLTPDQPFPKTRGNVIRSKDWNDAIVEVQRLDTAKVNKVGDTI
                     TGSLTVGASLNVGTTTAGSKLELGGNADLVLRATAEDPGDIVFQNSAGTQKGRVWSNP
                     TAGAALFLSSGDITPDLTVDASGNVGIGTTTPASGNKLDVAGNVRILTGSNPVRFTSA
                     HSGFSNSVTNQAEISNDTGTFMSLMIVGNRSGSLPGPGLGRRVSVYDTLEVNGTVRMA
                     IQGDILFKGGVDANHGIGWYGGAKGDRDSYDLGFANTTVDGPVVYGFNGGALGSTSGG
                     QKIALSWDSTGDVTMRGSLRFPNSTKPLMYIFESGDQNPERPVIAHSPPFPTWGLSYR
                     DVTDSMIFQAGGSPVVEVNLIGAVRLQVNGSAAKPGGGSWSVSSDERLKKRVRSLDNV
                     LDRLSRLRGVSFEWKKPEEQGGLTGTQLGLVAQEVEEVFPEWVDTDQNGYKMLTIRGF
                     EALTVEALKELVAENKTLQAKNTELEGRIEELEASASGQEQR"
     gene            complement(2145..2636)
                     /locus_tag="VHM69_11680"
     CDS             complement(2145..2636)
                     /locus_tag="VHM69_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741098.1"
                     /translation="MNDKQAMSGILTLRVSDREGRLVYEQPCKNRIVTSGRRLVAQMF
                     GGVTSGTPPSKVTHMAVGEGGTAPVDGDTGLRTQRDGRKPISEVTYEEFDEPIQGGSI
                     RRIRARLTAEFDFGEANGAEPLREAGVFNAATGGVMYNRVVFQDVTKTDAFKLTLLWD
                     IVF"
     gene            complement(2652..>2966)
                     /locus_tag="VHM69_11685"
     CDS             complement(2652..>2966)
                     /locus_tag="VHM69_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741099.1"
                     /translation="DYSGELFVFQGLPQERIQDVVDQTRAAGVRGNVIFTLDFYETHD
                     HREERYTTLAVHRASENLVADDALTLGSINRNAEAQDMGETFVIGGVWDVGTFDGSFG
                     LQ"
BASE COUNT          487 a          976 c          872 g          631 t
ORIGIN      
        1 acagttacaa ctctgaggca tcgccggatc gcgtcgactt tcctcgtcat gatttgaact
       61 cgttttcggc cctggcgatt acgccttcga caaaccagtt cttcgccgca gtgtaggcgg
      121 atcggtcaaa cctgtactcg acggcgagtt gttgtttcag ctcctggtag cttcgcgcca
      181 cgtccgggta cgcgcgcagt acgtcacgga acagcgtata tctgtgccag gtttccccgc
      241 ccagctctac catgtgcagg tgatacatgc actcgagaag ccaggcgccc ctccgaaaga
      301 agcgcctatc caggatcgcc ggcagcggga cgtacgcgta cccgatgttc actagcgagg
      361 cggtgcaagc gtccgccgtg gcagcgacgt tcctggtgcc gaccatcacg tcgatgatag
      421 gcttggccgt catctccggt accgccgtgc tgccgacgtg ctcgatcgcc aggacccgtg
      481 gaccgatggc gtccagtatc ctctccttct cggattcgaa gagcgccggc caccctggat
      541 cgtactcggc caggacgacg gtggggaaag tcacgactcg atccgctcca tcaaggggca
      601 ggttatcgtt ccgtggctcg tcacgcgatt gcacccctct tgctcgcatc accgttgttc
      661 ttgcccggaa gcgctagcct cgagttcctc tatcctgcct tccagctcag tgttcttcgc
      721 ttgcagggtc ttgttctcgg ccacgagctc cttgagggct tccacggtca gggcctcgaa
      781 gcccctgatc gtcagcatct tgtacccgtt ttgatccgtg tccacccact cggggaacac
      841 ttcttcgact tcctgggcca ccagccctag ctgcgtgcct gtcaggccac cctgctcctc
      901 gggcttcttc cattcgaagc tgaccccgcg caaacgcgag agcctgtcca gaacattatc
      961 cagtgacctg acgcgcttct tcagcctttc atcggatgaa acgctccacg agccgccccc
     1021 tggtttggcc gcactgccgt ttacctggag ccggaccgcg ccgatgaggt ttacctcaac
     1081 gaccggcgac ccgccggcct ggaagatcat cgagtcggtg acgtcccgat aagacaggcc
     1141 ccatgtgggg aaaggcggcg agtgggcgat gacgggccgc tcggggttct gatcgcccga
     1201 ctcgaagatg tacatcagcg gcttcgtgga gtttggaaag cgcagagagc cccgcatcgt
     1261 cacgtcaccg gtactgtccc aggatagcgc gatcttttgc ccgcccgagg tcgaaccaag
     1321 cgcgccgccg ttgaacccgt agaccaccgg gccgtccacg gtcgtattcg cgaatcccag
     1381 gtcgtaggag tctctatccc ccttggcccc accgtaccag cctatgccgt ggttcgcgtc
     1441 tacgccgcct ttgaacagga tgtcgccctg gatggccatc ctcacggtac cgttgacctc
     1501 cagtgtgtcg tacacggaga ctctccgtcc caggccaggg ccggggaggc tgccggatct
     1561 gttgccgacg atcatcaagc tcataaaggt gcctgtgtcg ttcgagatct cggcctggtt
     1621 cgtgacgcta ttggagaacc ctgagtgggc cgacgtgaat cggaccgggt tcgagcccgt
     1681 caatatcctg acgttccccg ctacgtccaa cttgttgccc gaagcaggcg tcgtcgtgcc
     1741 gatgccgacg ttcccgctcg cgtctacggt cagatccggg gtgatgtctc cgctgctcag
     1801 gaagagagcg gcgcctgcgg tcgggttgga ccagaccctt cccttctgcg tccctgcgga
     1861 gttctggaaa acgatatcgc cggggtcttc ggctgtggcc ctgaggacca ggtccgcatt
     1921 gccgcccaac tcgagctttg atccagccgt cgtagtacct acgttgaggc tggcgccaac
     1981 cgtaagcgag cccgtaatag tatcgccaac cttgttcacc ttggcggtat ccaggcgttg
     2041 cacctcgact atggcgtcgt tccagtcctt ggagcggatg acgttgcctc gggtctttgg
     2101 gaacggttga tcgggggtca aaaccatggt cgcctcctaa gagctcagaa tactatgtcc
     2161 cataacaggg tcagcttgaa ggcgtcggtc ttcgtaacgt cctggaagac gacgcggttg
     2221 tacatcaccc cgccggtcgc ggcgttgaag acaccggcct cccgcagggg ctctgcgccg
     2281 ttcgcctcgc cgaagtcgaa ctcggcggtg agcctggccc tgatgcgtct gatggacccg
     2341 ccctggatcg gctcgtcgaa ctcctcgtag gtaacctcgc tgatgggctt gcgaccgtca
     2401 cgctgcgtcc gcaggccggt gtcgccgtca accggggcgg tgccgccctc gccgaccgcc
     2461 atgtgggtta ccttcgaggg cggggtcccc gacgtgaccc cgccgaacat ctgggccacc
     2521 agcctgcgtc cgctcgtcac gatgcggttc ttgcaaggct gctcgtagac cagacgtccc
     2581 tccctgtcag acaccctgag ggtgaggatc ccgctcatcg cctgcttatc gttcatcgct
     2641 actccttcct cttactgcaa gccgaagctt ccgtcgaagg tacccacgtc ccacactccg
     2701 cctattacga aggtctcgcc catgtcttga gcctcagcat tccggttgat gctgccgagg
     2761 gtgagggcgt cgtcagcgac caggttctcg ctcgcgcggt ggacggccag cgtcgtgtac
     2821 cgttcctcgc ggtggtcgtg ggtctcgtag aagtcgagcg tgaagatgac gtttccgcga
     2881 acgccggcgg cccgggtctg gtccaccacg tcctggatgc gctcctgtgg caggccctgg
     2941 aatacgaaga gctcgccgct gtagtc
//
LOCUS       DATAIR010000485         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_60268, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000485 DATAIR010000000
VERSION     DATAIR010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60268"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VHM69_11690"
     CDS             complement(<1..906)
                     /locus_tag="VHM69_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2741100.1"
                     /translation="MTEISSRGAKTFDEEHREDPLWYKDAIFYQLHVKAFFDSDNDGI
                     GDFKGLTQRLDYIKDLGVTVIWLMPFYPSPMRDDGYDISEFKNVHPDYGTKRDFRAFV
                     RKAHELGLKVITELVINHTSYEHAWFQRARKAPKGSARRNYYVWSDTPDKYDGTRIIF
                     TDTERSNWTWDDEAGQYYWHRFFSHQPDLNHDNPQVFKSIMGVMRFWLDAGVDGLRLD
                     AIPYLIEREGTDNENLPETHDVLKRMRAEMDARYENRLFLAEANQWPEDVLPYFGDED
                     ECHMAFHFPLMPRIFMALAQEDRHPI"
     gene            complement(906..2849)
                     /locus_tag="VHM69_11695"
     CDS             complement(906..2849)
                     /locus_tag="VHM69_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX2741101.1"
                     /translation="MQIAFEPVIVENVYPELDCGRYAVKREVSDFFEVWADLFKEGHD
                     VLAAVLKYREKGASGWSETPMRLHENDRWRGGFTLTKNTRYEYTIEAWMDEFETWRRD
                     LGKKVDDGQRVQLELIEGRGIVEGAFSRSHDKRLKETLSGLDAAGYEGRLALLRSDEL
                     RKLMKEHPDRSMSTVYGKSLGIIVDRERARYGAWYEMFPRSQGTVPGRSATFGEAAQR
                     LPEIAGLGFDVVYLTPIHPIGSSHRKGRNNALKAGPDDPGSPYAIGSEEGGHKAVHPG
                     LGTLKDFRRFVQACHEHEMEVAIDLAIQCSPDHPYIKKHPEWFNFRPDGTIKYAENPP
                     KKYQDIVNVDFYSEDMENLWREWLDVILFWVEQGVKIFRVDNPHTKPFPFWEWAIGEV
                     QDEHPDVIFLSEAFTRPKVMKALAKLGFTQSYTSFTWRNPKQEIIEYLTELTQDWPKE
                     YMRPNFFPNTHDILPHILQFGGRPAFQMRFVLAATLSSTYGIYSGYELCENTPRGEPG
                     TTVYYQDSEVYEYKVWDWNRPGNIKEYIRRVNEIRRQNPALHYLKNLSFYAAQDDNIL
                     FYGKRTGDNAVFVAVNLDPHNAHDTHVQFPLWELGIEEDEGYVVEELMTEREIPATGS
                     WFWVRLDPRSNPAEIFRLRKVGA"
BASE COUNT          498 a          888 c          885 g          649 t
ORIGIN      
        1 gatggggtgc ctgtcttctt gcgcgagcgc catgaagatg cggggcatga gcgggaagtg
       61 gaaggccatg tggcattcgt cctcgtcccc gaaatagggc aggacgtcct cgggccactg
      121 gttcgcctcg gcgaggaaga ggcggttctc gtagcgggcg tccatctcgg cacgcatcct
      181 cttgagcacg tcgtgcgtct cgggcaggtt ctcgttgtcc gtgccctcgc gttcgatcag
      241 gtaagggatc gcgtctagcc tcaggccgtc aaccccagca tcgagccaga agcgcatcac
      301 gcccataatc gacttgaaga cctgggggtt gtcgtggttg aggtcgggct ggtgcgagaa
      361 gaagcggtgc cagtagtact ggccggcctc gtcgtcccag gtccagttag acctctccgt
      421 gtccgtgaag atgatgcggg tgccgtcgta tttatcaggg gtgtcgctcc atacgtagta
      481 gttgcgcctc gcggagccct tcggtgcctt gcgggctcgc tggaaccacg catgctcata
      541 agaggtgtgg ttgatgacca gctcggttat gaccttgagt ccgagctcgt gcgccttgcg
      601 cacgaatgcc ctgaagtccc gctttgtgcc gtagtcgggg tggacgttct tgaactcgga
      661 gatatcgtag ccgtcgtcgc gcatcggcga tgggtagaag ggcatgagcc agatcaccgt
      721 gacgccgagg tcctttatgt agtcgaggcg ctgcgtcagg cccttgaagt ccccgatgcc
      781 gtcgttgtcc gagtcgaaga acgccttgac gtgcagctgg tagaagatgg cgtccttgta
      841 ccagagcggg tcctcgcggt gctcctcgtc gaacgtcttt gctcccctgg atgagatctc
      901 cgtcactacg caccaacttt cctgagacgg aagatctccg ccgggttgct cctcgggtcg
      961 agcctgaccc agaaccagct gcccgtggct ggaatttccc tctccgtcat gagctcctcg
     1021 acgacgtaac cctcgtcttc ttctatcccc agctcccaca gcgggaactg cacgtgggtg
     1081 tcgtgggcgt tgtggggatc gaggttcaca gcgacgaaga cggcgttgtc tcccgtccgt
     1141 ttaccgtaga agagtatgtt gtcgtcctgg gccgcgtaga acgagaggtt cttcaggtag
     1201 tggagcgcgg ggttctggcg ccgtatctcg ttcaccctcc ttatgtattc cttgatgttg
     1261 ccaggcctgt tccagtccca gaccttgtac tcgtaaacct ccgagtcctg gtaatagacg
     1321 gtggtgcccg gctccccccg aggggtgttc tcgcacagct cgtaaccgct gtagataccg
     1381 tatgtgctcg acagcgtggc cgcgagcacg aagcgcatct ggaacgccgg cctgcctccg
     1441 aactgcagga tgtgcggcag gatatcgtgg gtgttgggga agaagttggg gcgcatgtac
     1501 tccttcggcc agtcctgcgt tagttccgtc aggtactcga tgatctcctg cttggggttg
     1561 cgccacgtga aggaggtgta agactgggta aagccgagct tggccagcgc cttcatcacc
     1621 ttcgggcggg tgaaagcttc ggagaggaag atcacatcag ggtgctcgtc ctgaacctca
     1681 cctatcgccc actcccagaa cgggaaaggt ttggtatgag gattgtccac cctgaagatc
     1741 ttgaccccct gctcgaccca gaacagaatg acatcgagcc actcccgcca gaggttctcc
     1801 atgtcctcgg agtagaagtc aacgttgacg atgtcctggt acttcttggg cgggttctcg
     1861 gcgtacttga tggtcccgtc cggcctgaaa ttgaaccact cgggatgctt tttgatgtaa
     1921 gggtggtcgg gcgagcactg gatggcgagg tcgatcgcga cctccatctc gtgctcgtgg
     1981 cacgcctgca cgaagcgtct gaaatccttt agcgtgccga ggccgggatg gacggccttg
     2041 tgtccaccct cctcgctccc gatggcgtag gggctgccgg gatcgtcggg accggccttc
     2101 agggcgttgt tccgcccctt cctgtgactg ctgccgatgg gatgtatcgg ggtcaggtac
     2161 acaacgtcga agccgagccc ggctatctca ggcaggcgct gcgcggcctc cccgaaagtc
     2221 gcgctccggc caggcaccgt cccctgcgaa cgggggaaca tctcgtacca cgccccgtag
     2281 cgcgcccgct cgcggtcgac gattatcccc aacgacttgc cgtatacggt ggacattgaa
     2341 cggtcgggat gttccttcat caacttgcgg agctcgtcgg agcgcagcaa agcgagcctg
     2401 ccctcgtaac cggctgcgtc gaggccggat agcgtctctt tgaggcgctt gtcgtgcgat
     2461 ctactgaagg cgccctcgac gataccgcgg ccttcgatca gctccagttg cacacgttgt
     2521 ccgtcatcta ctttcttgcc gagatcccgc ctccacgtct cgaactcgtc catccaggcc
     2581 tcgatggtgt actcgtagcg tgtgttcttc gtgagcgtga agccgccccg ccagcggtcg
     2641 ttctcgtgaa ggcgcatggg cgtctcgctc caccctgacg cgcccttctc cctgtacttc
     2701 aggaccgcgg cgagcacgtc gtgcccttcc ttgaagaggt cggcccagac ttcgaagaag
     2761 tcgctcacct ccctcttgac cgcgtatcgt ccgcagtcca gctcgggata gacgttctcg
     2821 acaatcaccg gttcaaaggc tatttgcacc ccgccccctc ttctagcaac tctcactagc
     2881 cttccaacaa acgcgccact ctaaacggaa gatgatctca
//
LOCUS       DATAIR010000486         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_308575, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000486 DATAIR010000000
VERSION     DATAIR010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308575"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VHM69_11700"
     CDS             complement(<1..1153)
                     /locus_tag="VHM69_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2741102.1"
                     /translation="MSGPGTWGKEDLQRRNHELSVLNEIARELNRSVELGEALGFTLS
                     QVAELLGLRTGWIWLMDDSSPGPYLAAARNLPPALADDPRRMDGAGYCYCLDTYKRGD
                     LEGAANVNVLTCSRLDGLVDGTGGLRYHASIPLYAGEKKLGVMNVASPQWRGLSPEDL
                     QLLSTIGDLLAIAVERARLFDRSARLGAVEERNRLAREIHDTLAQGLTATGLQLESAE
                     AFLDDESTVRRAREPLRRALLLTRSNLEEARRSVLDLRAAPLEGRSLPEALKTLVDRW
                     EAETGVATRFGAVNGSRPLPPRVEVALYRVCQEALTNVARHAHASRVTVRLVAIPEAV
                     HLLIMDDGRGFDPSSIADDRHGIVGMRERIEMLGGSLQVESSEGTRVEVT"
     gene            1289..1570
                     /locus_tag="VHM69_11705"
     CDS             1289..1570
                     /locus_tag="VHM69_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741103.1"
                     /translation="MGETGLARQLEDLFHEVGEAHHQAYIETDGADPEWPLWYADYLR
                     ERLGMLLDASFTRSELVYLLVLVASEQPYVAPGANWARYYAKKFISLYA"
     gene            complement(1609..1992)
                     /locus_tag="VHM69_11710"
     CDS             complement(1609..1992)
                     /locus_tag="VHM69_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741104.1"
                     /translation="MEDLAPRVSVMLVEDQADFRHLMATLLDRQPDLDLVAEAASLAE
                     ARTHAATKEVDVVVLDLGLPDGTGTDLISELRAGSPGTAVLILSASLNPEALAMATVA
                     GADEILDKFASLDEVVDTIRYLGGC"
     gene            complement(1994..>4042)
                     /locus_tag="VHM69_11715"
     CDS             complement(1994..>4042)
                     /locus_tag="VHM69_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741105.1"
                     /translation="PVRGTWIAGRIYPTRAGLSVYFRDVTERRRTEEGLGFLAEASEV
                     LSSSLDYRTTLASVARLSVPALSDWCAVDIVGDDGSLERLAVEHADREKIELAYKLEE
                     RYPTDPDAPGGVHEAIRTGEPRMMAEIPPELVDQAARDEEHREMLRKLGLRSYMVVPL
                     VARGKTLGAISFVTAESGRIYTEADLELAQELARRAALAVDNAWLYEEAQKEIEERRW
                     AQEELRGSRNQLEIILGGVADGITAQDANGQLFYANEAAARMCGYSSVRAFVEAPVEE
                     VLRKFEILDEAGDPFPIERLPGRRALRGEEDAEALLRFRVLETGEERWTLVRAMPVLD
                     EAGRVRMAVNIMRDMTEQRRAQLEQARLAAIVASSEDAIISKTLDGIITSWNRGAQRI
                     YGYSPEETVGQPISMLVPPENPNEIPGILEKLRRGERIEHYETVRVSKDGRRLDIALT
                     ISPIRDAAGNVTGASTIARDITERKRTQEALREIRESERRRLARDLHDGALQDLAYTT
                     AAMGLMMLKAQDTSLAGELQGVVDAVRRAARGLRDVVNDLRLEQDRPLPELVASLVQM
                     NRVMARGREISLEVAEDFPSTPLGETGTQMLRVIQEALTNARRHSGARKIVVSLKSEG
                     TDLVVEVSDDGQGFGQGTAQGVGLSSMRERATAIGGELEIESESGTRIRLLVPSPEEG
                     "
BASE COUNT          632 a         1363 c         1252 g          795 t
ORIGIN      
        1 ctgtaacctc gacacgcgtg ccctcgctgc tctccacctg gagggagccg ccgagcatct
       61 ctatcctttc cctcattcct acgatgccgt gcctgtcgtc cgcgatgctc gacgggtcga
      121 agcctcgtcc atcgtccatg attagaagat gcaccgcctc cggtatggct acgagccgga
      181 ccgtcacgcg gcttgcgtgg gcgtggcggg cgacgtttgt gagggcctcc tggcagacgc
      241 ggtagagggc gacctcgacc cgcggcggga gcgggcggct accgttcacc gccccgaacc
      301 gggtggccac gcctgtctca gcttcccagc ggtccaccag ggtcttcagc gcctccggca
      361 gtgagcggcc ttcgagcggg gcggcgcgga ggtcgaggac ggagcggcgg gcttcttcga
      421 ggtttgagcg ggtgagcaag agggcgcggc gcagcggttc gcgggcgcgc cgaaccgtgg
      481 attcgtcatc gaggaaagct tcagccgact ccagctggag gcccgttgcc gtgagtccct
      541 gggcgagggt gtcgtggatc tcacgcgcca gtcggttgcg ctcctcgacg gcgccgagcc
      601 tggcgctgcg gtcgaagagg cgggcacgct cgacggctat ggcgagcagg tcccctatcg
      661 ttgagaggag ttgcaggtcc tccggggaga ggccgcgcca ctgcgggctc gcgacgttca
      721 tgacgcccag cttcttctcg cctgcgtaca gggggatgct ggcgtggtag cgcaggccac
      781 cagtgccgtc caccaggccg tcgaggcggc tgcaggtcag gacgttgacg ttggccgcgc
      841 cttcgaggtc gcctctcttg taggtgtcca ggcaatagca gtagcccgcg ccgtccatgc
      901 ggcgcgggtc gtcagccagg gccggaggga ggttgcgggc ggcggcgagg tacggcccag
      961 gagaagaatc gtccatcagc cagatccaac ccgtacgcag ccccagtagc tccgcgacct
     1021 gcgacagagt gaacccgagc gcctcgccaa gctccacgga gcggttgagc tcgcgggcga
     1081 tctcgttcag caccgagagc tcgtgattgc gccgttgcag atcctctttc ccccaggtgc
     1141 cgggcccgga cacacctact ccagtctcta tgttcgaagc tgtttatgag tttatcagcg
     1201 cgcaaagacg gctggccgtt tcgcaagttg cggggcttcc gggccggaaa tatacttgcg
     1261 cgtgttgcgt agtggcgagg aggacgagat gggggagacg ggacttgcac gtcagctgga
     1321 ggatctgttt cacgaggtag gcgaagccca tcatcaggct tacatcgaaa cggacggcgc
     1381 cgaccccgaa tggccgctat ggtacgcgga ctatctacgg gagaggctcg gcatgctcct
     1441 cgatgcaagc ttcaccagga gcgagctcgt ctatctactc gtcctcgtcg cgagcgagca
     1501 accctacgtt gctcccggcg cgaactgggc cagatactac gccaagaagt ttatctctct
     1561 ttacgcatga acagggttcg agtactcggg gcgacgatgt ggacgcgttc agcagccccc
     1621 gaggtatctt attgtgtcga cgacctcgtc aagagatgcg aacttgtcca atatctcgtc
     1681 cgcgcccgct actgtggcca tcgccagagc ctcggggttc aggctggcgc taaggatcag
     1741 gacggcagta cccgggctgc ctgcccgcag ctctgagatc aagtccgttc ccgttccgtc
     1801 agggaggccc aggtcaagta cgaccacgtc cacttccttg gtcgccgcgt gcgtgcgcgc
     1861 ctcggcaagc gatgcggcct cggccaccag atcgaggtca ggctgcctgt ctagcagtgt
     1921 cgccatgagg tgcctgaagt cggcctggtc ctcgaccagc attacgctga ctcgcggggc
     1981 gagatcttcc accctatccc tcctccggcg aagggaccag caaccttatc ctggttccac
     2041 tctcgctctc gatctccagt tcaccaccga tagcggttgc ccgctcgcgc atgctgctca
     2101 ggcccacgcc ctgggccgta ccctgcccga acccttgccc gtcatcggag acttctacca
     2161 cgagatccgt cccttccgac ttcaagctaa ccacgatttt cctggcccca gagtggcgtc
     2221 tggcgttggt cagcgcctcc tggattacgc gcaacatctg cgtcccggtc tcacccagcg
     2281 gcgtcgaggg gaaatcttcg gccacctcga ggcttatctc gcggccccgg gccatcaccc
     2341 ggttcatctg caccagggaa gccaccagct cgggcagagg acgatcctgc tccaggcgca
     2401 ggtcgttgac cacgtcgcgc agcccccttg cggcgcgtct tacggcgtcg accaccccct
     2461 gaagctcccc agccaggctc gtgtcctggg ccttgagcat catgagcccc atggcagccg
     2521 ttgtgtaggc aaggtcctgc aaggcaccgt cgtgcaggtc gcgggccagc cggcggcgct
     2581 cggattccct gatctctcgt agagcctctt gagtccgctt gcgctcggtt atgtcgcggg
     2641 cgatcgtgga ggcccctgtt acgttgccag cggcatccct gatgggggag atggtcagcg
     2701 cgatatccaa cctcctgccg tccttgctca cgcgtactgt ctcgtagtgc tctatcctct
     2761 ccccgcgcct gagtttctcg aggattcctg gtatctcgtt ggggttctcg ggcggtacca
     2821 gcatggagat gggttgtccc acggtctcct ccggggagta gccgtagatc ctctgcgctc
     2881 ccctgttcca gctcgtgatt atgccatcca gcgtcttgct gattatggcg tcttcggatg
     2941 acgccactat ggcggcgagc cgggcttgct ccagctgggc gcgtctctgt tcggtcatgt
     3001 cgcgcatgat gttgacagcc atgcggaccc ttcccgcctc gtccaggaca ggcatcgccc
     3061 tgaccagagt ccaccgctct tcacccgtct ccaggacgcg gaaacgcagc agcgcttcgg
     3121 catcctcctc gcctctgagc gccctccgtc ctggcagcct ctcgatcggg aacggatctc
     3181 ccgcctcgtc caggatctcg aactttctca atacctcttc gacgggtgct tccacgaacg
     3241 ccctcaccga tgagtagccg cacatcctgg ctgccgcctc gttggcgtag aatagctgac
     3301 cgttcgcgtc ctgggcggtg atgccatccg ccaccccgcc gagtatgatc tcgagctggt
     3361 tcctggaacc ccgcagctcc tcttgcgccc accggcgctc ttcgatctcc ttctgggctt
     3421 cctcgtagag ccatgcgttg tccaccgcga gtgcggccct gcgtgcgagc tcctgcgcca
     3481 actcgaggtc agcctccgtg tagatgcggc ccgattcggc ggtcacgaag gatatcgcgc
     3541 cgagggtctt tccacgtgcc acgagcggca ccaccatgta agacctaagg ccgagtttcc
     3601 tcagcatctc cctgtgttcc tcgtcgcggg cggcctggtc cacgagctcc ggtggtatct
     3661 cagccatcat ccgcggctcg ccggtcctga tcgcctcgtg taccccgcct ggagcgtcgg
     3721 ggtcggtcgg gtagcgttcc tcgagcttgt aagccagttc gatcttctcg cgatcggcgt
     3781 gctccacagc cagccgctcc agggacccgt cgtcgccaac tatgtccacg gcgcaccaat
     3841 cggagagggc cggaaccgaa aggcgcgcga cgctggccag cgtcgtcctg tagtccagcg
     3901 aggaagatag cacctcgctc gcttcggcaa ggaagcccag accttcttcg gtccgcctgc
     3961 gctctgtcac gtcccggaag tagacggaaa gtcccgcccg agtggggtag attcgtcccg
     4021 cgatccacgt cccccgcaca gg
//
LOCUS       DATAIR010000487         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_400885, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000487 DATAIR010000000
VERSION     DATAIR010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400885"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VHM69_11720"
     CDS             <1..848
                     /locus_tag="VHM69_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX2741106.1"
                     /translation="ANAVWGQQGYRFEDAYLRTLAADYGAGLRTIDFGKPEAASEEIN
                     AWISSRTEDRIKDLVSPDIIDPTTRLVLANAVYFKASWLSRFEKSSTQDGPFTPLDGG
                     ETTIPMMHQTTYLDYADGEGYQAVRLPYKGEAADMLVILPDEGRFETVEERLNSDFLD
                     EVDRSLDTKYVSLTMPRFDFETDLDLEERLKAMGMTRPFDAADFSGISGAKDLYISDA
                     LHRANITVDEKGTEAAAATVLVMAESSTPQVTEMTMDRPFIFAIVERDTGTILFLGRV
                     TNPQK"
     gene            928..2208
                     /gene="larA"
                     /locus_tag="VHM69_11725"
     CDS             928..2208
                     /gene="larA"
                     /locus_tag="VHM69_11725"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX2741107.1"
                     /translation="METQLQYGKGDMSVEIPSSDVTVLVPKAEEGLPDEATAFREAVR
                     DPIGSAPLREIVPSDERIAVVIPDITRALPRERLLPLLFEELSHIPPENFTIVNGTGS
                     HRANTGEELATMVGREVFSNYRVVNHDAHDPATMELVGKSHTGRDLYMNKEYVQADRR
                     IVMGFIEPHFMAGFSGGYKGIFPAVADVDSIMDYHRASVIGHERSTWGVLEDNPTQDQ
                     IRHNGSLLPLDFCINVTLNRAQEITGFYCGDVLAAHAEGCEANKSTSMVACESEFPIV
                     VTTNGGYPLDQNLYQAVKGMSAAYQVVAEGGLIICAARCNDGFPDHGNFRKMLLEYPS
                     PEAMLEAINSPGFSTYDQWQVQLFAQILQRARVALHSEIDAEAVRRVHLEPVDDITTR
                     ISEELENIGANAPIAVLPEGPMTVPYLEHAQIRA"
     gene            2310..2564
                     /locus_tag="VHM69_11730"
     CDS             2310..2564
                     /locus_tag="VHM69_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2741108.1"
                     /translation="MSRATLTSKGQITVPKDVRERLGLKSGDRVAFEFEGDSVRLRVE
                     KSKSLEELKGSLPATREYTGKESERKAAWEHATNEVLGGS"
     gene            2561..2977
                     /locus_tag="VHM69_11735"
     CDS             2561..2977
                     /locus_tag="VHM69_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2741109.1"
                     /translation="MSRDVSSWWLDANVLLRFLTGAPPDLAERAARLLEEAQQGNVFL
                     RVHPVVVAETVWVLESFYGYSKAEISRVLIPLLEQPSLRVEGARSVIEALEAMAESNV
                     DFADALLAVTARSRGEGVASFDWDFRKLDVAWREPG"
     gene            complement(3008..>4095)
                     /locus_tag="VHM69_11740"
     CDS             complement(3008..>4095)
                     /locus_tag="VHM69_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741110.1"
                     /translation="QLDLNLHPNEASGLLTIGDETLLGEHGWDLGFWAVLDEGEIEDC
                     VAAIGHRRDSPMAEGWEIERLHADVRGNNGKTEDAEAITRDDSTGYVYILGSHFGSKA
                     GPLQPKRGFVARFREADVGHVTEDPAMQLDVYRRSFALHRLVNDALKESGPDLIPLGE
                     QTYKALISATIKRGKKEEKKWAGLVCEDDYPINIEGAAFRENGTLLLGLRFPTSADGH
                     PILIELESIDRLFESGDDLPEVRGFWTADAVGKNGEIAGVRDLALMQTERGEELHLVT
                     GNVDSRDKQSVLIQDYPGGRDTVATHFRCILPPGAHSGSLEAEFVREFPNLPRIEGIA
                     LAPEGRVFYVTDEDEGVHLRLTRLIAG"
BASE COUNT          799 a         1252 c         1292 g          752 t
ORIGIN      
        1 tcgccaacgc ggtgtggggg cagcaagggt accggttcga ggatgcttat ctgaggacgc
       61 tggccgcgga ctacggggcg ggcctgcgca ccatagactt cgggaaaccg gaggcggcat
      121 cggaggaaat aaacgcctgg atctcctccc ggaccgaaga ccgcatcaag gatctcgtct
      181 cgcccgacat catcgacccg acgacgcggc tggtgctggc caacgccgtc tacttcaagg
      241 catcgtggtt gtctcgcttc gagaagtcaa gcacgcagga cggccctttc accccgctcg
      301 acggcggcga gacaaccatc cccatgatgc atcaaaccac gtacctcgac tacgctgatg
      361 gtgagggcta ccaggccgtc cggctacctt acaaaggcga ggcggcggac atgctcgtca
      421 tcctgcccga tgagggccgc ttcgagacgg tcgaagagcg cctgaactca gactttctcg
      481 acgaggtaga ccgtagcctc gacacgaaat acgtcagcct gaccatgcca cgcttcgact
      541 tcgagacaga cctcgacctc gaggagcgcc tcaaagcaat ggggatgacg agaccattcg
      601 atgccgcgga cttcagcggc atcagcgggg cgaaggacct gtacatctcc gacgccctgc
      661 acagggccaa catcacggta gacgagaagg gcaccgaagc cgccgcagct acggtgctgg
      721 taatggcgga gagttccact cctcaggtga cagagatgac catggaccgt cctttcatct
      781 tcgccatagt cgagcgtgat acgggtacga tcctgttcct tggaagggtg acgaacccac
      841 agaagtagcc ttgcggcgac catccccctg tccgcatgta gaatggaacc ggggactgct
      901 cgtttttatg tcagggggtt actgagggtg gagacgcagc tacagtacgg caagggcgac
      961 atgagcgtgg agatcccatc ttccgacgtt acggtgctgg tgccgaaggc ggaagaaggt
     1021 cttcctgatg aggcgacagc gttcagggag gcggtcaggg accccattgg atcggcgccg
     1081 ctgcgggaga ttgtcccatc cgacgagcgt atcgcggtgg tgatccccga catcacccgc
     1141 gcccttcccc gcgaacgact cctgcctctt ctcttcgagg agctctcgca tataccgccc
     1201 gagaacttca ccatagtgaa cggcacgggg tcccacaggg ccaacaccgg cgaggagttg
     1261 gcgacgatgg tcggacggga agtattttcg aactaccgcg tcgtcaacca cgatgcccac
     1321 gaccccgcga cgatggagct tgtcggcaag tcgcacacgg ggcgggacct gtacatgaac
     1381 aaggagtacg tccaggccga caggcgcatc gtcatggggt tcatcgagcc tcacttcatg
     1441 gccgggttct cgggcgggta caaggggatc ttccccgccg tggctgacgt ggattcgatc
     1501 atggactacc acagggcctc ggtcatcggc cacgagcgca gcacgtgggg cgtccttgag
     1561 gacaacccga cgcaggacca gatccggcac aacggctccc tccttcctct cgacttctgc
     1621 ataaacgtaa ccctcaaccg agcgcaggag atcaccggct tctactgcgg cgatgtcctg
     1681 gccgcccacg ccgagggctg cgaggccaac aaatccacct cgatggtcgc ctgcgagagc
     1741 gagttcccta tcgtcgtcac cacgaacggc ggctaccccc tggatcagaa cctctaccag
     1801 gccgtcaagg gcatgtccgc cgcataccag gtcgtcgccg aaggcggcct cataatctgc
     1861 gccgcccgct gcaacgacgg cttccccgac cacggcaact tccgcaagat gctcctcgaa
     1921 tacccctcgc cggaagcgat gctcgaagca ataaactcgc cgggcttctc gacctacgac
     1981 cagtggcagg tacagctctt cgcccagatc ctgcaacgag ccagggtcgc cctccacagc
     2041 gagattgacg cagaagccgt ccgccgcgtc cacctcgaac cagtcgatga catcactacc
     2101 cgaatctctg aagaactgga gaatatcggt gcaaacgccc ccatcgccgt cctccccgaa
     2161 ggccccatga ccgtcccgta tctggagcac gcccagatcc gagcataaga gtggagatct
     2221 taacctcaac cctcatatcc cgggcaggtg catggtccac acatcttcgg atgctggtag
     2281 aatcagttta tcaagtaagg agactagata tgtcaagggc aactctgaca agtaagggac
     2341 aaattaccgt tccgaaagac gtgcgggaac gtttgggctt aaagagtggg gaccgcgtgg
     2401 cgttcgagtt cgagggcgac tcggtccgtc tcagggtcga gaagagcaag agcctcgaag
     2461 agctaaaagg cagtctgccg gctaccaggg aatacacggg gaaggagtcc gagaggaaag
     2521 cggcgtggga gcacgcgacg aatgaggtgc tcggcgggtc ttgagccggg acgtttcttc
     2581 ctggtggttg gatgcgaacg tgctcttgcg tttcctgacc ggggctccgc ccgatctcgc
     2641 cgaacgtgcg gccaggcttc tggaggaggc gcagcagggt aatgttttcc tccgggtgca
     2701 tcccgtggtc gtggcggaga cggtgtgggt gctcgaatcc ttctacggtt attcgaaggc
     2761 tgagatctcc cgtgtcctca tacccctgct ggagcagccg agtctgcgcg tcgagggagc
     2821 aagaagcgtc atcgaagcgc tcgaagcgat ggcggagagc aacgtggact tcgccgacgc
     2881 cctgctcgcg gtgacggccc gctcacgcgg cgaaggtgtc gcctctttcg actgggactt
     2941 tcgcaagctc gacgtagcat ggcgcgaacc aggctagttg gcgacgacat gagaaccggg
     3001 cggcgcttca gcctgcgatc aggcgcgtga gccgcaggtg gacaccttca tcctcgtccg
     3061 tgacgtagaa gacgcggcct tcgggagcga gggctatccc ttcgatgcga gggagattcg
     3121 ggaactcgcg gacgaactca gcttcgaggg agcctgagtg ggcgccaggc gggaggatgc
     3181 agcggaagtg ggtggctacg gtgtctctgc cccctgggta gtcctggatc aggacgctct
     3241 gcttgtcgcg gctgtcgacg ttgccggtta cgaggtgtag ctcctcgcct ctctcggtct
     3301 gcatcagggc gaggtcgcgg acgccggcta tctcgccgtt ctttccgacg gcgtcggcgg
     3361 tccagaatcc gcggacttct gggagatcgt cgccactttc gaagaggcgg tcgatgcttt
     3421 cgagctctat cagtatcggg tgtccgtcgg cggaggtggg gaagcggagg ccgaggagta
     3481 gggtgccgtt ctcacggaag gccgcgcctt ctatattgat cgggtagtcg tcctcgcaca
     3541 cgaggccggc ccacttcttt tcctccttct tgccgcgttt gatggtagcc gagatcaggg
     3601 ctttgtaggt ttgctcgccg agaggaatga ggtcggggcc gctctctttg agggcgtcgt
     3661 tgacgagccg gtggagggcg aagctccgcc ggtagacgtc gagctgcatc gcggggtctt
     3721 ccgtgacgtg cccgacgtcg gcctcgcgga agcgggcgac gaagccgcgt ttgggctgca
     3781 gcggtccggc cttgctcccg aagtgggagc cgaggatgta gacgtagccc gtgctatcgt
     3841 cgcgtgtgat cgcctccgcg tcctcggtct tgccgttgtt tccacgcaca tcggcatgca
     3901 gacgctctat ctcccaaccc tcggccatag gggaatcgcg gcggtggccg atagcggcga
     3961 cgcagtcttc gatctccccc tcgtcaagta ccgcccagaa accgaggtcc caaccgtgct
     4021 ccccgagcaa cgtctcgtcc ccaatcgtca gtagccccga cgcctcgttc gggtgcaggt
     4081 tgagatccaa ctgaa
//
LOCUS       DATAIR010000488         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_60393, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000488 DATAIR010000000
VERSION     DATAIR010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60393"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VHM69_11745"
     CDS             complement(<1..321)
                     /locus_tag="VHM69_11745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741111.1"
                     /translation="MAEERNRLRKVFDEVALLYDGARPGYPGELFDDIVTLSGIPLEG
                     RILEIGCGTGQATVPFARRGYHVVCIELGENMAALARRNLKGHPQVRTGDFEQMPLQE
                     RAFDL"
     gene            complement(331..798)
                     /locus_tag="VHM69_11750"
     CDS             complement(331..798)
                     /locus_tag="VHM69_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEX2741112.1"
                     /translation="MRTSRGLALGAISGLRSFSGPAFVSRAASQGYLKLGGSPLAFLG
                     SKRLSQALMVMALGELVGDKSSITPSRTSPPVLLWRAASGGLVGAASFVSEDRSAATG
                     VALGSIAAIAAAIAGEWLRALAGRKTGLPDPVVALAEDAVVIFVGFRSLKEVR"
     gene            complement(795..1871)
                     /locus_tag="VHM69_11755"
     CDS             complement(795..1871)
                     /locus_tag="VHM69_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2741113.1"
                     /translation="MDPKTIVVMEGDQTGQELLEEALRVLDPAVTGLDLDFTRYDLSL
                     ENRRATGNEVVHQAAAAMKETGFGIKAATVTPEDPDDVGSPNAILRKGIDGTVIVRTG
                     RCIPGVNPTSGVHAPISVVRMAVDDAYGAKEWREGQGQDEVAYRTEKISRRVCRGVSE
                     FAFIQARKMRAKVFGGPKWTVSPVYEGMFKEEMDRAAENSRDVRYDPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLISLDEEENPETVMAEAPHGTA
                     PSLQGKNVANPMAMILAGAALLTFFHEEDASRVSRAIYEATFETVLDGVRTADLGGSA
                     GTTEFTDEIIRHVRTKLEVWSALA"
BASE COUNT          320 a          618 c          611 g          367 t
ORIGIN      
        1 cagatcaaag gctctctctt gcaagggcat ttgctcgaag tctccggtac gcacttgcgg
       61 atggcctttc agattgcggc gggcgagggc ggccatattt tcgccgagtt cgatgcagac
      121 gacgtggtag ccgcggcggg cgaagggcac ggtagcctgg cccgtgccgc atccgatctc
      181 gagtatccgc ccttcgagag gtattcctga gagcgtcact atgtcgtcga agagctcgcc
      241 gggatagccg ggacgtgccc cgtcgtagag cagcgccacc tcatcgaaga ccttccttag
      301 ccggttcctt tcttcggcca tgagcgtagg ttaacgtact tccttcaacg agcggaatcc
      361 gacgaaaatc acgacggcgt cttcggcgag cgcgactacg ggatcgggga gccccgtctt
      421 ccgccccgcg agcgcgcgca gccattcgcc tgcgatggcc gccgcgatag ccgcaatgga
      481 gccgagggcg acgcccgtag cggcgctccg atcttccgag acgaacgagg cggccccaac
      541 gagacctccg gaggccgcgc gccagagcag gacaggaggc gaggtacggc tcggagttat
      601 ggacgattta tccccgacga gttctcccag cgccattacc atcagcgcct gcgagagacg
      661 cttcgagcca aggaaagcga gcgggctccc tccgagcttc aggtatccct ggctcgcggc
      721 gcggctcacg aaggcagggc cggagaacga gcgcaggcct gagatcgcgc ccagagccag
      781 tcccctggac gttctcacgc gagcgcgctc cacacttcga gtttggtacg cacgtggcgt
      841 atgatctcat cggtgaactc ggtcgtgccc gcggagccac cgaggtctgc ggtcctcact
      901 ccgtcgagga cggtctcgaa ggtcgcctcg tagatggcgc gcgagacgcg gctcgcatct
      961 tcttcgtgga agaaggtgag gagggctgct ccggccagga tcatggccat cgggttggcc
     1021 acgttcttgc cctgaagtga aggcgccgta ccgtgcgggg cttcggccat gacggtctcc
     1081 gggttctcct cctcatccag cgaaatgagg agcgactcgg ccccggcgat ggagccgaac
     1141 atctgcatca cgaggtctga gatgcagtcg ccgtcgcggt tcaatgtcgg gatgacgagt
     1201 ggctcgccgt aggtggtgag gagcagcgcg taggtggcgt ctatgagttg ggggtcgtag
     1261 cggacgtcgc ggctgttttc ggcggcgcgg tccatctcct ccttgaacat cccctcgtag
     1321 accggagaga cagtccactt ggggccgccg aagaccttgg cccgcatctt gcgtgcctgg
     1381 ataaacgcga actcggagac gccccggcat actcgacggg agatcttctc cgtcctgtag
     1441 gcgacctcgt cttgcccctg accctcgcgc cactccttcg ccccgtaggc gtcgtcgacg
     1501 gccatgcgga ccacggagat cggggcgtgc acgccgctcg tcgggttgac gccggggatg
     1561 caccgtcccg tgcgtacgat gacggtcccg tctatacctt tcctcaagat ggcgttcggc
     1621 gaccccacgt cgtcagggtc ctcaggcgtt acggtcgccg cctttatgcc gaatcccgtc
     1681 tctttcatcg ccgcggccgc ctggtgaacg acctcgttgc ccgtggccct ccggttctcg
     1741 agcgacaggt cgtagcgcgt gaaatccagg tcgagccccg ttaccgcagg gtcgagtact
     1801 ctcagggcct cctcgagcag ctcctgaccc gtctggtcac cctccattac aacgatcgtc
     1861 tttgggtcca ctcgtcgcac ctctgtgaat agtctctgtg aatgatcctg ctaagt
//
LOCUS       DATAIR010000489         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_308787, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000489 DATAIR010000000
VERSION     DATAIR010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308787"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHM69_11760"
     CDS             <1..441
                     /locus_tag="VHM69_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2741114.1"
                     /translation="QPGTGDSVQALKAGVMEIADIFCINKADNPQARNAASEVRSILE
                     IGQELDPDPWFPPIVMTRGDTGEGVEELKDTIEKHRRFLEEDGRLAERRRAALRDFVV
                     SWATNRLEKEMHERLNREDTELVEKVYNRELDPISASERIFKEV"
     gene            445..1194
                     /locus_tag="VHM69_11765"
     CDS             445..1194
                     /locus_tag="VHM69_11765"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX2741115.1"
                     /translation="MAPQRIVSLVPSLTETLFALGVGERVVGRTRYCTQPPRAVGSVA
                     KVGGTKKVDVTRVLGLEPDLIVAVKEENTLEDVEALADAGVPIFLGAPETVAGAVDML
                     RELAARIEAPRAEEVLAPIERVVKRLRVQSEESRRVFVPIWKSPYMSAGSDTYLHDVL
                     TTCGGENVFGDRTRYPVVTHYEIEAAQPEVILLPDEPYPFSAEDLPEFYTLDCPAARG
                     DRIHLIDGKLLTWYGPRMAGSLTQIAALLRF"
     gene            complement(1191..>1744)
                     /locus_tag="VHM69_11770"
     CDS             complement(1191..>1744)
                     /locus_tag="VHM69_11770"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX2741116.1"
                     /translation="PALDRRHSGVLRGGDVHRVPDPPRRLMASGALRTLAALGCGLVF
                     GLGLAVSGMMNPAKVIGFLDVAGDWDPTLVFVMGGALLVTLPAYRVILKRGRPVLADG
                     FTVPTKKSLDAPLIWGSTLFGVGWGLVGFCPGPAVAAIGTGLPSVLAFVAAMIAGMAL
                     QAWISIERTVGKGSSSDAAPNPR"
BASE COUNT          361 a          531 c          591 g          261 t
ORIGIN      
        1 caaccgggaa cgggggactc ggtgcaggcg ctgaaagccg gggtgatgga gatcgccgac
       61 atcttctgca tcaacaaggc ggacaacccc caggccagga acgccgcctc cgaggtgcgc
      121 tctatactgg agatcgggca agagctcgac cctgacccgt ggttcccgcc catcgttatg
      181 acccgcggcg ataccggcga gggcgtagag gaactgaaag atacgataga gaagcatcgc
      241 cggttcctcg aagaagacgg caggctcgca gaaaggcggc gggctgccct gagagacttc
      301 gtcgtctcgt gggcaacgaa ccgcctggag aaggagatgc acgagcgcct taaccgcgag
      361 gatacggagc tcgtcgagaa ggtctacaac agggagctcg accccatctc ggcctcggag
      421 cgtatcttca aagaagtcta gggcgtggcg cctcaacgca tcgtcagcct cgtcccgagc
      481 ctgacggaga cgctcttcgc cctcggcgtg ggcgagaggg tagtgggccg cacccgctac
      541 tgcacacagc cgccacgggc cgtcgggagt gttgcgaagg tcggcggcac caagaaagtg
      601 gacgtaacgc gcgtcctcgg gttggagcca gatctcatcg tcgccgttaa ggaggagaat
      661 acactggagg acgtggaggc gctcgccgac gcgggcgtcc ccatctttct cggcgcgccg
      721 gagacggtcg ctggggctgt ggacatgctc cgggagctcg cggcaaggat cgaggctccg
      781 cgtgcggagg aagtactcgc acccatcgag cgcgtcgtga agaggttgcg tgtgcagagc
      841 gaagaatcgc gccgggtctt cgtcccgatc tggaagagcc cgtacatgag cgctggaagc
      901 gatacctacc tccacgacgt gttaacgacg tgcggcggag agaacgtgtt cggggaccgg
      961 acgcgctacc ctgtcgtcac gcactatgag atcgaggcgg cgcagcctga agtcatcctg
     1021 ctgccagacg agccgtaccc cttctcggcc gaggacctgc cagagttcta cacactcgac
     1081 tgtccagccg ctcgtgggga taggatccac ctcatagacg gcaaactcct gacgtggtac
     1141 ggaccccgca tggcggggag cctcacccag atcgctgcgc tcttgcgatt ttagcgaggg
     1201 ttcggggcgg cgtcggacga cgagcccttg ccgactgtgc gttcgatgga gatccaggct
     1261 tgcagcgcca tgccggcgat catggcagct acgaaagcta gtaccgacgg aagccctgtg
     1321 cctatagcgg cgaccgccgg accggggcag aacccgacga gaccccaccc gacgccgaag
     1381 agggttgagc cccagatcag tggcgcgtcg aggctcttct ttgtaggcac ggtgaagccg
     1441 tctgccagga cgggacgccc ccgtttcagg atcacacggt atgcgggtaa ggtgacgaga
     1501 agagcgccgc ccatgacgaa gacgagggta gggtcccagt ccccggccac gtcgaggaag
     1561 cctatgacct tggccgggtt catcatccct gagacggcga gcccgagacc gaagacgaga
     1621 ccacagccca gggcggcgag agttctcaga gcaccggagg ccatcagacg acgtggcggg
     1681 tcaggaacac ggtgaacatc gccaccccga agaacaccgc tgtggcgacg atcgagcgcc
     1741 ggga
//
LOCUS       DATAIR010000490         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_337013, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000490 DATAIR010000000
VERSION     DATAIR010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337013"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VHM69_11775"
     CDS             complement(<1..290)
                     /locus_tag="VHM69_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX2741117.1"
                     /translation="MDRGYVGTGVKPVRRVSRRRFLYGAATALGGMALASCSGGPFGS
                     SKQKIHFWNLFGGGDGARLADMESDFIQAHPEIDLSATTLTWGAPYYTKVSMA"
     gene            complement(390..2183)
                     /locus_tag="VHM69_11780"
     CDS             complement(390..2183)
                     /locus_tag="VHM69_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX2741118.1"
                     /translation="MKPRPEYPRPQFRRQHWTNLNGEWSFAFDDSNVGLAKAWQDVTP
                     DDLRSGNSPFDRRITVPFCYQSERSGIGETAFHDVVWYACSFEHTPAEGERLLLHFGA
                     VDYRATAWVNGVYVASHEGGHTPFSADVTHALKGEGNVIVVRAEDPSQDVTIPRGKQY
                     WKEESEGIFYTRTTGIWQTVWLEPVNRSRVGSLRLTPNVDAASIELEAAVEGIEPGMS
                     LRVSVDLDGEPVLEDTFRLHSSFVERSIPLVRRGEAPGESRLAAWTGIALWSPEDPNL
                     YDLRLELLDEGGEVLDTVESYVGMRKIETKEGKVYLNGRPYYQRLILDQGYFPEGILT
                     APTDDDLRGDIELAKEMGFNGARKHQKVEDPRWLFWADTLGFLVWGEMANAYQYSPDY
                     VRRITAEWQEAVIRDYSHPCIVAWVPMNESWGVPNLVARRDQVSHLLALYHLTRSLDQ
                     TRPVVSNDGWEHAMTDLCTIHDYRDADALAKSYVTTESTIASEPANRPIYLPGHGYRG
                     EPVLITEFGGIAFSGDEKGWGYSTVANADEFLESYESLIDALLHSEPVQGFCYTQLTD
                     VEQEVNGLLTYDRRPKADLTRIREITSRPKP"
     gene            complement(2266..3861)
                     /locus_tag="VHM69_11785"
     CDS             complement(2266..3861)
                     /locus_tag="VHM69_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2741119.1"
                     /translation="METETDILIVGGGLGGVAGALAALRLGKRVILSEETDWIGGQLT
                     VQAVPPDENPWIEEMGCTASYRRLRNGIRDYYRRNYPLTDEARAREDFNPGDGLVSRL
                     CHEPRVALAVLEGMLAPYVSGHRLQMLLRHRPIAAESEGDLIETVTLLDEETGEPLTV
                     HARYVLDATELGDLLELAGVEHVVGAESQDETGEPHAPPGRPNILDQQAVSWCFPLDY
                     MPGEDHTIDKPEDYDFWRGYRASFWPGPQLAWTDVYPHTLQPRHRAIFHEEPFFEGRD
                     GSDLWHYRRILARENFSEGHLDSDITLVNWPHIDYWLGPLVGVGEQERHKHLEGAKQL
                     SLSMLYWMQTEAPRLDGGEGYRGLRIRPDVVGTRDGLARRVYVRESRRIRAEFTVLEQ
                     HLGVEARGEVSGAETFNDSVGIGAYRIDLHPSAAGRSYVDVSSWPFQIPLGALIPVRA
                     ENLLPACKNLGTSHITNGCYRLHPVEWNIGEASGALAAYCMERDLVPRQVRTEYLEDF
                     QHLLAEVLGFELAWPEPQRCEAL"
     gene            complement(3885..>4176)
                     /locus_tag="VHM69_11790"
     CDS             complement(3885..>4176)
                     /locus_tag="VHM69_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2741120.1"
                     /translation="AFDRGETFSGVDFETGLRAAEELKGLVPEGYTLAQFALRWILMH
                     PAVSCAIPGGKNPSQVEDNVASSEMPALSDKVMDRVREVYDSYIRTEVHHKW"
BASE COUNT          701 a         1324 c         1326 g          825 t
ORIGIN      
        1 gccatcgaga ccttcgtgta gtacggggcg ccccacgtta gcgttgtcgc cgagagatcg
       61 atttcagggt gggcctgtat gaagtcgctc tccatgtcgg caagccgagc gccatcgccg
      121 ccaccaaaga ggttccagaa atggatcttc tgcttcgaac tgccaaatgg tccccccgaa
      181 caggaagcca gcgccatgcc accgagagcc gtcgcagcac cgtaaaggaa ccgccgcctg
      241 ctgacccggc gcacgggctt gaccccggtc ccgacgtacc ctcggtccat acccttcccc
      301 tttcaggcga acgctcgcct tacctgtgcc gcatgttatc atccgttacc gagatggtca
      361 acaaaggttc gtggggagtc tggcccgagt taaggcttcg ggcgggacgt tatctccctt
      421 attcgggtga gatccgcctt cggccttctg tcgtaggtga gcaggccgtt gacttcctgc
      481 tccacatctg tgagttgcgt gtagcagaag ccctggaccg gctcgctgtg cagcagggcg
      541 tcgatgagcg actcgtagct ttccaggaac tcatccgcgt tagcgacggt actgtagccc
      601 cagcctttct cgtcgccgga gaatgcgatg ccgccgaact cggtgatcag gaccggttcg
      661 cccctgtagc cgtgaccggg aaggtagatg ggccggttcg cgggctcgga ggcgatcgta
      721 gattctgttg tgacgtagct cttcgcgaga gcatccgcat cgcggtagtc gtggatcgtg
      781 cataggtcgg tcatggcgtg ttcccagccg tcgttcgaga cgacgggcct cgtctggtcg
      841 agcgagcgcg tcaggtggta gagggcgagg aggtgggaga cttgatctct tcgcgccacc
      901 aggttaggga cgccccaact ctcgttcatc gggacccagg cgacgataca cgggtggctg
      961 tagtcgcgta tgacggcctc ctgccactcg gccgtgatgc gcctaacgta gtccgggctg
     1021 tactggtagg cgttcgccat ctcgccccag acgaggaaac cgagggtatc cgcccagaag
     1081 agccagcgcg ggtcctcgac cttctggtgc tttcgcgcgc cgttgaaacc catctccttc
     1141 gcaagctcta tgtccccgcg caggtcgtcg tcggtagggg cggtcaggat tccttcgggg
     1201 aagtatccct ggtcgagtat gaggcgctgg tagtagggtc tcccgttcag gtagactttg
     1261 ccttccttcg tctcgatctt cctcatcccg acgtagctct cgacggtatc gagtacctct
     1321 cccccctcgt cgagcaactc cagtcgcagg tcgtagaggt tggggtcctc agggctccag
     1381 agggcgatgc ccgtccatgc ggccaggcgc gactcgccgg gcgcctcgcc gcgtcgtaca
     1441 agaggaatgc ttcgttcgac gaacgaggag tgcagtcgaa atgtgtcttc cagcaccggc
     1501 tccccgtcca gatcgacgct cacgcgcagg ctcatacccg gctcgatgcc ttcgacggcg
     1561 gcttcaagct cgatgctggc ggcgtccacg ttgggggtaa ggcgcaggga cccgacgcgg
     1621 ctgcggttca cgggctccag ccacacggtc tgccagatcc ccgtcgtcct ggtgtagaag
     1681 atcccctcgg attcctcttt ccagtactgc ttgccccgag ggatggtcac gtcctgcgaa
     1741 ggatcctccg cccgaactac gatcacgttc ccttcaccct tcagcgcatg cgtcacgtcc
     1801 gcagagaacg gcgtatggcc gccctcgtgc gaggccacgt atactccgtt cacccaggcc
     1861 gtcgcccgat aatccaccgc gccgaagtga aggagcagtc gctcgccctc tgccggcgtg
     1921 tgctcgaagg agcaggcgta ccagacaacg tcgtggaagg ccgtctcgcc gatgccggat
     1981 ctctcagact ggtagcagaa ggggacggtg atcctccggt cgaacggcga gttaccggag
     2041 cgcaggtcgt ccggggtcac gtcctgccag gctttcgcga ggccgacgtt cgaatcgtcg
     2101 aaggcgaagc tccactcacc gttcaggttg gtccagtgct ggcggcggaa ttgagggcgc
     2161 gggtactcgg gacgtggctt caagctgcgg tccccctttc tgctagagat gtgggccatt
     2221 cgttcggcgt ggcggacgga gatcctcgat ggggcggtgt ccttatcaaa gggcctcgca
     2281 gcgttgcggc tccggccagg caagctcaaa gccaaggacc tcggccaaca aatgctggaa
     2341 gtcttccagg tattctgtcc gcacctgcct cgggacgagg tctctttcca tacagtaggc
     2401 ggcgagtgcc cctgatgcct caccgatgtt ccactccaca ggatgtaacc tgtaacagcc
     2461 gttggtgatg tgggaagtac caaggttctt acaggccggc agcaggtttt cggcccgcac
     2521 cgggatcagg gctcccagcg ggatctggaa cggccagctg cttacatcca catagctgcg
     2581 tccggcagcg ctgggatgca ggtctatgcg gtatgcgccg atgcctacgc tgtcgttgaa
     2641 ggtctctgcg ccgcttacct cgcctcgcgc ctcgacgcct aagtgctgct ccagcactgt
     2701 gaactcggcc cggatgcggc ggctctctcg gacgtagacg cgcctggcga ggccgtccct
     2761 ggtgccgacg acgtcgggac gaatgcgcag gcccctgtat ccttcgcccc cgtcgagcct
     2821 gggtgcttcg gtctgcatcc agtacagcat cgagagactg agctgcttcg caccttccag
     2881 gtgtttgtgg cgctcctgtt ctccgacgcc aacgagcggg ccgagccagt agtctatgtg
     2941 tggccagttg acgagggtga tgtcgctatc gagatgcccc tcggaaaagt tctcacgggc
     3001 gagtattcgt ctgtagtgcc agaggtcgga gccgtcacgc ccctcgaaga agggttcctc
     3061 gtggaagata gcgcggtggc ggggctgcaa ggtatgaggg tagacgtcgg tccaggctag
     3121 ttgcgggccg ggccagaaac tcgcgcggta gccacgccag aaatcgtaat cctcgggctt
     3181 gtctatcgtg tggtcctcgc cgggcatgta gtcgagtggg aagcaccagc tcaccgcctg
     3241 ctggtccagg atgttcggcc tgcccggcgg cgcgtgaggc tcgccggtct cgtcctggct
     3301 ctccgcccct accacgtgct cgacgccggc cagctccagc aagtctccga gctcggtagc
     3361 atcgagcacg tagcgggcgt ggacggtcag cggctcgccg gtctcctcgt cgagaagtgt
     3421 aacggtctcg atgaggtccc cttcgctctc ggcggcgatg gggcggtggc ggagcagcat
     3481 ctggagcctg tggccggaga cgtagggagc cagcatccct tcgagcacgg cgagggctac
     3541 gcgcggctca tggcagagtc ggctcaccaa tccatctccc ggattgaagt cctccctggc
     3601 gcgggcttcg tccgtgagcg ggtaattgcg gcggtagtaa tccctgatgc cgttcctcaa
     3661 gcgacggtag ctcgcggtgc agcccatctc ctcgatccag ggattctcgt ccggcggaac
     3721 ggcctggacg gtgagctggc cgccgatcca atccgtctcc tctgagagga tgacccgctt
     3781 gcccaggcgc agggcggcca gagcgccggc cacaccccca agccccccgc cgacgatcag
     3841 tatgtcggtt tcggtctcca atggcgacta accccctccg caggctacca cttgtgatgg
     3901 acctccgtcc ggatgtagct atcgtagacc tcgcgcacac gatccatgac cttgtcggag
     3961 agcgcaggca tctcggagga ggcgacgttg tcttcgacct gagaggggtt cttgccgcct
     4021 gggatcgcgc acgatacggc ggggtgcatc aggatccaac gcagcgcgaa ctgcgccagc
     4081 gtatatccct cagggacgag acctttcaac tcctccgcag cccgaaggcc ggtctcgaaa
     4141 tctacgccgg aaaaggtctc accgcggtcg aaggcc
//
LOCUS       DATAIR010000491         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_31696, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000491 DATAIR010000000
VERSION     DATAIR010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31696"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VHM69_11795"
     CDS             <1..822
                     /locus_tag="VHM69_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741121.1"
                     /translation="LGGDDPDSVELYRKEVLEKNVSLIDLLEEFMACEVPFNVYLELV
                     PLLKPRYYSISSSPLVDPERCSITVGVVDAPARSGRGRYQGVCSSYLAKQPEGSVLEG
                     FVRPPSTPFVPPEDPATPMVMVAAGTGLAPFRGFLQERAAIKHEQGKEVGMSLLFFGC
                     RNPKEDYIYEEELQEFARGGLTHLECAFSRVEGQPKMYVQNALKANNEEVLRLVGEGA
                     VIYLCGDASRMAPAVEKAFIELYREERGVGEQEGEAWMRDLKDSKRYVVDVWPRS"
     gene            835..>2303
                     /locus_tag="VHM69_11800"
     CDS             835..>2303
                     /locus_tag="VHM69_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2741122.1"
                     /translation="MEDSGPGAIGTGGYLPIAEHGLIGDLYTVALVGTNGTIDWYCCP
                     RFDAPSLFGAILDKERGGYWAIAPASTGWTTKQLYIPDTNVLITRFLSPDGVGELQDF
                     MPIAARGYRPHCMIRRVVCVRGRMPFRLEVEPRFDYGRSQHETILHEQGAVFTSPELS
                     VALGSPLPLERSEKGARARFTLKAGESATFALDLVNEGEEPRVYEEGDTQRAFEDTVD
                     FWRRWLSRSRYAGRWREMVHRSALTLKLLTYSPTGAIVAAPTTSLPEQLGGGRNWDYR
                     YTWIRDAAFSLYGLLRLGFTEEAGAFMGWLTERFREGRFSSTGPLQLMYGIDGRSELI
                     EETLDHLEGYRGSAPVRVGNAAADQLQLDIYGELIDSVYLYNKYGTPIFHDAWEDLSR
                     VVEWVCEYWDEPDEGIWETRGGRKRFVHSRLMSWVAIERCVRMARQRGLPGNLARWIP
                     ERDRIYRQIMRQGWNEQRRAFVQHYDTDVLDASVLLMPLV"
BASE COUNT          432 a          685 c          768 g          418 t
ORIGIN      
        1 ctcggtggcg atgatcccga cagcgtggag ctctacagaa aggaggtcct tgagaagaac
       61 gtctccctga tagacctgct tgaggagttc atggcttgcg aggtgccttt taacgtctac
      121 ctcgagctag tgccgcttct gaagccacgc tactactcga tctcatcctc tcctctggta
      181 gatcctgagc ggtgcagcat cacggttggg gtggtggatg cgcctgcgcg ctctggcagg
      241 ggacgctacc agggggtctg ctcctcctac ctcgccaagc agcctgaagg aagtgtgctc
      301 gaagggttcg tgcgtccccc gagcacgccc tttgtgcctc ctgaggaccc tgcaacgccc
      361 atggtgatgg tcgcagcggg cacgggactc gctcccttcc gcgggttcct gcaggagcgg
      421 gcagcgatca agcacgagca gggcaaggaa gttggcatgt cgttgctgtt cttcggctgc
      481 cgcaatccca aggaggacta catctacgag gaggaacttc aggagttcgc aagaggagga
      541 ctaacccacc ttgagtgcgc gttctcaagg gttgaagggc agcccaagat gtatgtgcag
      601 aacgctctga aggcgaacaa cgaggaagta ttgcgcctgg tcggagaggg tgcggtcatc
      661 tatctgtgcg gggatgcaag caggatggct ccagcggtgg agaaagcctt catcgagcta
      721 tacagggagg agagaggggt cggcgagcag gaaggcgagg catggatgcg ggatctgaag
      781 gactccaagc ggtacgtggt cgacgtctgg ccgcgaagct gacctcttca agatatggaa
      841 gatagcggac ctggagcgat cgggaccggc ggttacctgc cgatcgctga gcacggcctg
      901 atcggtgacc tctatacggt cgcgctggtc ggtaccaacg gcacgattga ctggtactgc
      961 tgcccgcgct tcgacgcccc gagcctgttc ggggcgatcc tggacaaaga gcgcggcggc
     1021 tactgggcta tcgcccccgc ctccacggga tggacgacca agcagttgta catccccgac
     1081 accaatgtcc ttatcacgcg tttcctcagt cccgacgggg tgggcgagtt gcaggacttc
     1141 atgcctatcg cagcgcgggg atacaggcca cactgcatga tccggcgcgt cgtctgcgtg
     1201 cgcggccgga tgccttttcg cctggaggtc gaaccacgtt tcgactacgg acggagccag
     1261 cacgaaacga tcctgcacga gcagggggcc gtcttcactt cgcctgagct gtcggtggca
     1321 ctgggatcgc cgcttcctct ggagcgctcg gagaagggtg cgcgtgcgcg cttcacgctc
     1381 aaggcgggag agagcgccac cttcgctcta gatctcgtca acgaagggga agagccgagg
     1441 gtctacgaag agggggacac gcagagggcg ttcgaggaca ccgtggattt ctggcgacga
     1501 tggctctcgc gctcgcgcta cgcggggcgc tggcgggaga tggtgcaccg ctccgcactc
     1561 accctcaagc ttctgaccta ctccccgacc ggcgccatcg tggcggcgcc tacgacgagc
     1621 ctgccggaac agctcggcgg agggcgcaac tgggactatc gctacacctg gatccgggac
     1681 gccgccttct cgctctacgg cctcttgcgc ctcggcttca ccgaggaggc cggcgcgttc
     1741 atgggctggc tgaccgagcg cttcagggag ggacggttct catcgacagg accattgcaa
     1801 ctcatgtacg gtatcgacgg gcgctcggaa ctcatcgagg agacgctcga ccatctcgag
     1861 ggctacaggg gctccgcgcc cgtacgggtg ggcaacgcgg cggccgatca actccagctc
     1921 gacatctacg gagagctgat cgactcggtc tacctttaca acaagtacgg cacgccgatc
     1981 ttccacgacg catgggaaga cctctcgcgc gtcgtcgagt gggtgtgcga atactgggac
     2041 gaacccgacg agggtatctg ggagacgagg ggcggacgta agcgcttcgt ccactccagg
     2101 ctgatgagct gggtcgccat cgagcgctgc gtaaggatgg cacggcagcg cgggttgccg
     2161 ggcaacctgg cgcgttggat ccccgagcgc gaccgcatct accgccagat catgcgtcag
     2221 ggctggaacg agcaacgcag ggcgttcgtg cagcactacg atacggacgt cctcgacgcc
     2281 tccgttctgc tcatgccgct ggt
//
LOCUS       DATAIR010000492         6327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_60529, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000492 DATAIR010000000
VERSION     DATAIR010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60529"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VHM69_11805"
     CDS             complement(<1..642)
                     /locus_tag="VHM69_11805"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2741123.1"
                     /translation="MESLFDTAEGGENREQRLEELARQVSVCTKCDLAKTRTNTVFAT
                     GDPYSPLMLVGEGPGENEDATGLPFVGRAGKLLDDILAAVDLTRQQIYLTNIVKCRAA
                     VEEGGRLRNRQPKTVEIRACNPYLQGQIEAVKPDIILCLGGPAAKTIIDKDFRITKDR
                     GKWYEIDGIKTMATFHPAYVLRQRGDDLNNTKRLVWKDIQAVYAEYQKAMEERG"
     gene            815..1870
                     /locus_tag="VHM69_11810"
     CDS             815..1870
                     /locus_tag="VHM69_11810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2741124.1"
                     /translation="MKPRAALDTIRPYQPPRMAWEMEVDRGSKEYAKLTMNELSFGPL
                     PEAKAAAAESLSRGNRYPERDADPLREAISASNQGITKSHVILGNGSSEVLVDLLQIL
                     ERPGEVIFPWPSFPFYSSAARVVGLEERRVALDRSHTVDLDALYAATGPNTRAVILCN
                     PNNPTATYLTLDRVRTFAEALPEEVLLILDEAYYEFVEDPEYAGSHELVLESSNVVST
                     RTFSKVHGLAGFRIGYGMAQPEIADYVWRAHVPFSVSLVAQAAAEASMRQTEAIAERA
                     RFIRGERERLQAAFEEAGLDYVPSHTNFILVHSGPEVFERAGVLVREGEALGCPGWSR
                     ISLGSTAENDRILAALG"
     gene            2133..3785
                     /locus_tag="VHM69_11815"
     CDS             2133..3785
                     /locus_tag="VHM69_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2741125.1"
                     /translation="MATRSAESGRTEEHRLHRDVSTVGLLFVCLGSVIGSGWLFGALY
                     AAQIAGPASIISWVLGAIVMLILALVHAEVGSMYPVAGGSARYPHFAFGNLAGFATGW
                     IVWIGAVTVAPIEVLAALQYLTHYFPWLTDTAEGVTLLTPVGIVISIILMALFTVINL
                     LGVAALAKSNNVIMIWKIAIPFLAVAVIMILSFHTTNFVHPSHGGFMPFGIQGVLSAI
                     ATGGIIFSYQGFEQAIQFGGETRNPGRNVPLAVIGSMLVGLVLYILLQVAFLGALEPS
                     NIKNGWDEITFPGLAGPFAGLATAVGATWLATLLYIDAAVSPGGTGLLYSASSARLSF
                     ALARNHYIPRQFGYLSERGVPTVSIIFGFLIGCLMFLPFPGWQVLVGFIVSAAVMGYA
                     MVPLAFGALRRQEPDHPRPFKLPAGELLAPVAFIVANLIIYWTGWEILWKLIVAIALG
                     FVLLGIGHIVNPSELTPSFDWRASSWLWPYLGGMAVVSYIGASDFGGRGWIPFGWDIL
                     VVTAFSVAIYYYAISVRLTPDEVRRHVADAREEAEEEEQLAV"
     gene            complement(3814..5103)
                     /locus_tag="VHM69_11820"
     CDS             complement(3814..5103)
                     /locus_tag="VHM69_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX2741126.1"
                     /translation="MVKLDDDLADAIRGAVQARREDAVRLLQELVRIRSVTGEEGAVG
                     EVVGRAFSERGLEVDRWEATREEMEPYRDHVGEQSSYENRPNVAGVMRGTDGGRSILL
                     NAHTDTVDPGDPAAWKGDPLSGDLEGDLLYGRGSCDMKGGLVTHLVALDALSDLGLGL
                     LGDVTVAATVGEENRGLGALSTVLRGYRADAAIITEPTRLALVPAQGGSLVFRLKVQG
                     RSAHAAVRDEGVSALEKFVPIFENLRELEQERNGVLSHPLYEGLQNKVPINVGVVRSG
                     SWASTVPESLVAEGRVGLIPGEEVDPFKDLVAERIATVADRDPWLREHQPELEWFGGQ
                     FAPAEVPPDAPICEAVRKAHERATGVAPAVEGVPYGADMRLFIHFGDMPCVMYGAGDV
                     NVAHAPDEHISVTELLTATTTVACLLADWCGVDSEEG"
     gene            5485..6318
                     /locus_tag="VHM69_11825"
     CDS             5485..6318
                     /locus_tag="VHM69_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2741127.1"
                     /translation="MTGRSRSLFYAVLVVAVTLVIVVGCSAGTEEPFEAAAPGDETTG
                     PAAIVDREITVASNIAYPPFEFSPRDQPRGFDIDLMNEVADRADFEVEYENVHFDSIL
                     RGLTADLFDASISAMTITKEREQQIDFSDPYFNADQALMVSSDSEVQSIDELSEASIG
                     VQAGSTGQLKAEELLDEGQIREIIPYRTIGEAFAALRGGKVEGVIYDLSAAHREVVDS
                     KEKIRFVEPVPTGEQYGIAFPEDSALVEPVNEALAEIKADGTYERLYKKWFGMPPEDV
                     P"
BASE COUNT         1142 a         2000 c         1946 g         1239 t
ORIGIN      
        1 tcctcgctcc tccatcgcct tctggtactc ggcgtagacc gcctggatgt ccttccatac
       61 caggcgcttg gtattgttca ggtcgtctcc gcgttgcctc aggacgtagg ccgggtggaa
      121 ggtcgccatc gtcttgatgc catctatctc gtaccacttg ccgcgatctt tggtgatcct
      181 gaagtctttg tctatgatgg tcttggccgc gggaccgccg aggcaaagga tgatgtctgg
      241 cttgacggcc tcgatctggc cctgcaagta cgggttgcag gcccttatct cgaccgtctt
      301 cggctggcgg ttcctcaacc tgccgccctc ctctacggca gccctgcact tgacgatgtt
      361 ggtcaggtag atctgctgcc tggtcaggtc caccgccgcc aggatgtcgt cgaggagctt
      421 gccggccctc ccgacgaaag gcagccctgt ggcgtcttcg ttctctccag gcccctcgcc
      481 gacgagcatc agcggagagt aggggtcccc ggtcgcgaat accgtgttgg tgcgggtctt
      541 cgccaggtcg cacttggtac agacggagac ctgtcgggcc agttcttcca acctttgctc
      601 gcggttctcg ccgccctccg cagtatcgaa aagactctcc atggtccctc caggatgtag
      661 cgtacctatg ctattctaca ctgcgcactt cggaccctgg ggagcacggt gattgagatt
      721 cgtgaggtta gcatgggctt catcgacgaa gtgtgcaccc ctgagagtac gcgcaccagg
      781 cacttcctga atcagatccc aaaatgagag ttcgatgaag ccaagggccg cactcgacac
      841 catacgccct taccagccgc cgaggatggc ttgggagatg gaggtcgacc gcggttccaa
      901 ggagtacgcg aagctgacca tgaacgagct atccttcggc cctctccccg aggcgaaggc
      961 tgcggccgcc gaatctctct cgcgaggaaa ccgctacccc gagcgcgacg cagatcctct
     1021 gcgcgaggcg atctcagcct ccaaccaggg tataacgaag tcgcatgtga tcttgggcaa
     1081 cggctcgagc gaagtgctgg tcgatctgct gcagatcctg gagcgtcctg gagaggtgat
     1141 cttcccctgg ccctcatttc ctttctattc ctccgccgcc cgagtcgtcg gcctcgaaga
     1201 acgcagggtc gccctcgaca gaagccacac cgtagacctc gacgccctgt acgcggctac
     1261 aggtcccaat accagagctg tgatcctgtg caacccgaac aacccgacgg cgacgtacct
     1321 gactctcgat agggtacgca ctttcgccga ggcgctccct gaagaggtac ttctgatcct
     1381 cgacgaggct tactacgagt tcgtcgagga ccctgagtac gcaggatcgc acgagctggt
     1441 cctcgaatcg tccaacgtcg tctcgacaag gaccttctcg aaggttcacg ggctcgccgg
     1501 gttcaggatc ggctacggca tggcacagcc ggagatcgcc gactacgtct ggcgggccca
     1561 cgtccccttc agcgtgagcc tggtggcgca ggccgcagct gaagcctcga tgcgccagac
     1621 ggaggccatc gccgagcggg cacgtttcat aagaggagag cgcgagcgcc tgcaggctgc
     1681 cttcgaggag gcgggcctcg attacgtgcc ctcgcacacc aacttcatcc tggtccattc
     1741 agggcctgaa gtattcgaga gggccggagt tctcgtgcgc gaaggcgagg cgctcgggtg
     1801 tcccgggtgg agcaggatct ccctcggcag cacggctgag aacgacagga tcttagccgc
     1861 tctcggctga gaaaggtacc cgcagcaccg cccgagcccc gattatcctc ctgatccggc
     1921 gcgcacttgc cccggaccga ccaatgaccc acaatgccga aagctttcaa aagggaagga
     1981 ggcttagaaa atgctctccg agagaaacta cgcgacctgg ccgtagtcga gtacgtctgc
     2041 gcggtcggcg tcggatcgga cggagaccgc aacgagtgga caggaacaag tcttgacgga
     2101 tcaattccac cacgaataca ggaggcttgc ctatggctac gcgaagtgcg gaatcgggga
     2161 gaacggagga gcacaggcta cacagagacg taagcacggt cggcctgctc ttcgtctgtc
     2221 tgggatcggt catcgggtca gggtggctgt tcggggcgct atacgcggcg cagatcgcgg
     2281 gtccggcttc gatcatctcc tgggtgctcg gcgccatcgt gatgctcatt ctggcgctgg
     2341 tccacgcgga ggtcgggagc atgtacccgg tggctggagg ctcggcccgc tatccccact
     2401 tcgctttcgg gaacctcgcc gggttcgcga cggggtggat cgtgtggatc ggggccgtca
     2461 cggtggcccc catcgaggtc cttgccgcgc tgcagtacct cacccactac ttcccgtggc
     2521 tcacggacac ggcggaaggt gtgacgctct tgacaccggt tggcatcgtc atttccatca
     2581 tactgatggc cctgttcacg gtcataaacc tcctcggtgt ggccgccctg gcgaaatcga
     2641 acaacgtgat catgatctgg aagatcgcga tcccgttcct cgcggtagcc gtcataatga
     2701 tcctgtcctt ccataccacc aacttcgtcc atccctccca cggtggcttt atgcccttcg
     2761 ggatacaggg cgtacttagc gcaatagcca cagggggcat aatcttctcc taccagggat
     2821 tcgagcaggc gatccagttc ggaggggaga cgcgcaatcc tgggcgcaac gtcccgctcg
     2881 ccgtcatcgg gtcgatgctg gtcggcctcg tgctttacat cctgttgcag gtcgccttcc
     2941 tgggcgcact cgagccgagc aacatcaaga atgggtggga tgagatcacg ttcccggggc
     3001 tcgcaggacc cttcgccggt ctcgcgaccg cggtgggcgc aacctggctc gccacgctcc
     3061 tgtacatcga cgccgcggtc tcgcctggtg ggaccggcct tctctactcg gcctcgagcg
     3121 cgcgcctctc tttcgccctc gcccgcaacc actacatccc gaggcagttc ggctacctca
     3181 gcgagagggg cgtgccgacc gtcagcatca tcttcggctt cctgatcggc tgcttgatgt
     3241 tcctgccttt ccccggatgg caggtgctcg tcggctttat cgtctctgcg gccgtgatgg
     3301 gctacgccat ggtgccgctc gccttcggcg cactgaggcg ccaggagccc gaccatccac
     3361 ggcccttcaa gctgcctgcg ggcgagttgc ttgcgcctgt agccttcatc gtggctaact
     3421 tgatcatcta ctggaccggc tgggagatcc tctggaagtt gatcgttgcc atcgccctcg
     3481 gtttcgtgct actcggcata ggtcacatag tcaacccttc ggagttgacc ccctcgttcg
     3541 actggagggc cagttcgtgg ttgtggcctt acctcggggg gatggccgtc gtctcctata
     3601 tcggcgcatc ggacttcggc ggcagggggt ggatcccatt cgggtgggat atcctcgtcg
     3661 tgacggcctt cagcgtcgcc atctactact acgcaatatc ggtgcgcctc acgccagatg
     3721 aggtccgaag gcacgtcgcc gacgccaggg aggaagcaga ggaggaagaa cagctcgcgg
     3781 tgtgacgccg catggaggga taggggcagg tccctatccc tcttcagaat caacgccgca
     3841 ccagtccgca aggaggcaag cgaccgtagt tgtcgcggtc aggagctccg tgacgctgat
     3901 gtgctcgtcg ggggcgtgcg ccacgttcac gtcccctgcg ccgtacatga cgcagggcat
     3961 gtctccgaag tggatgaaga gccgcatgtc agcgccgtag gggacgccct cgacggcggg
     4021 cgccacacca gtcgcccgtt catgagcctt tctgacggcc tcgcagatcg gggcgtcggg
     4081 ggggacctcg gcgggtgcga actggccgcc gaaccactct agctcaggct ggtgttccct
     4141 aagccacggg tcgcggtcgg cgactgtggc gatgcgttcg gcgacgaggt ccttaaaagg
     4201 atctacctcc tcgccgggga tcaggccgac gcgtccctcg gcgacgagag actctgggac
     4261 ggtcgaggcc caactgccgg accgcacgac gccaacgttg atgggtacct tgttctgtaa
     4321 tccttcgtag agcgggtggc tgaggacacc gttgcgttcc tgctccagct cgcgtaggtt
     4381 ctcgaagatg ggtacaaact tctcaagcgc cgagacgccc tcgtcgcgga ctgcggcgtg
     4441 ggccgagcgg ccctggacct tgagcctgaa cacgagtgag ccgccctggg ccggcacgag
     4501 cgcgaggcgg gtcggctctg tgatgatggc ggcgtcggcg cggtacccgc gcaggacggt
     4561 cgatagggcg ccgaggcccc tgttctcctc cccgaccgta gccgccacgg tgacgtcgcc
     4621 gagcagcccg agaccgagat ccgaaagggc gtcgagcgcg acgaggtgcg taacgagccc
     4681 acccttcata tcgcaggagc cgcgtccgta gagcagatct ccctccagat cccctgagag
     4741 cgggtcccct ttccaggcgg cagggtctcc gggatctacg gtatcggtgt gggcgttgag
     4801 gagtatggag cgcccgccat cggttcctct catgacgccg gcgacgtttg gcctgttctc
     4861 gtaggagctc tgttcaccga cgtgatccct gtacggttcc atctcctcac gcgtcgcctc
     4921 ccaacggtct acctccagac cgcgctccga aaaggcacgc ccgacgactt cgcctacggc
     4981 accctcctcg cccgtcactg aacggatgcg caccagctcc tgaagcaacc gtactgcgtc
     5041 ctcccgcctg gcctgtacgg ctccacgtat agcatcagcg agatcgtcgt cgagcttcac
     5101 catgtgtcct cccttccggt tcccggcacc gtagcacgag gtcagttctt cggcaacgcg
     5161 ccgccggact tgcgcgagtg gtgatatacg ctaccaatca tgtgatggaa attgcgacgt
     5221 tactggcgat ccaatccgcc aacgggctgg agcggcgcct gaaccgcaga acggcgtaga
     5281 ctgcgcgcgg gcacagggtc cggacgcgac agtctccctc tacttgacca gatcacgtct
     5341 tgtaattagt attgtcgttt tggagggtca gcaaggagat gccgtaccca gctaacttgc
     5401 cggcggccgg gtccgtccgt gatcgccctt tccatcgcgg ctgggttaga ctacatgaca
     5461 ggaatacggg aggataggtt cacggtgaca ggacggtcga ggagcctctt ttacgcggtg
     5521 ctcgtcgtcg ccgttacgct cgtcattgtg gtcggttgca gcgctggaac cgaagagccc
     5581 ttcgaggctg ccgctccggg ggatgaaacc acgggtcctg cggcgatcgt ggacagagag
     5641 atcacggtcg cctccaacat cgcctacccg cctttcgagt tctctcccag ggaccaaccg
     5701 agagggttcg acatagatct catgaacgag gtcgcggaca gggctgactt cgaggtcgaa
     5761 tacgaaaacg tccacttcga cagtatcttg cggggactca ccgccgacct cttcgatgcg
     5821 tccatctcgg cgatgaccat aaccaaggag cgcgagcagc agatagattt ctcagaccct
     5881 tacttcaacg ccgaccaggc gctcatggtt tccagcgact cggaggtcca gtctatcgac
     5941 gagctctcag aggcatccat cggcgtccag gccggctcga caggccagct caaggccgaa
     6001 gagctactcg acgaagggca gatacgagag atcattccct acaggaccat cggagaggcc
     6061 ttcgctgcgc taaggggcgg taaggtcgag ggtgtgatct acgatctttc agccgcgcac
     6121 agagaagtcg ttgacagcaa agagaagatc aggttcgtcg agcccgtccc tacgggcgag
     6181 cagtacggca tagccttccc cgaagacagc gctctcgtcg agccggtcaa cgaggcgctg
     6241 gcggagatca aagccgacgg cacttacgag aggctataca agaagtggtt cgggatgccc
     6301 cccgaagacg taccctaggg tcagagg
//
LOCUS       DATAIR010000493         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_124766, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000493 DATAIR010000000
VERSION     DATAIR010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124766"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VHM69_11830"
     CDS             <1..540
                     /locus_tag="VHM69_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2741128.1"
                     /translation="CYGEVDLMERNDMDMSGMGTNLASLRERRMWTQVRLAEEAGVSP
                     TTVSGIENGRISRPHFGTLRKLAVALRVEPEELLPRPGVGRHENLSLEWSMASDDEEF
                     ERGLEDASIEGLSSLSDELDEEKVRLRRLYGEARGDVQRRLIKGMIRQVAANSGSVEA
                     SIMAHPDNEDVDEDAVRHE"
     gene            complement(764..2416)
                     /locus_tag="VHM69_11835"
     CDS             complement(764..2416)
                     /locus_tag="VHM69_11835"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2741129.1"
                     /translation="MADLSTKKAGYVAAEVLAGAGVRRLYTVPGESFLEILDAAEEHP
                     DLGLISTRHESGAAFMAEAEGKLTGIPAVAMATRGVGASNLAIGVHTARQDSTPMLVL
                     LGQVETDFLGREAFQEVDLTAFYAPITKWAATVHRADRLAEFVARGLRIATSGRPGPV
                     MLALPADVLGEQVQPGPDARVPEPPRPVPSSDQVRAVARRLSAAHRPVVIAGGGARNA
                     RETLISFAEAFDIGVYASFRRQDVFPNEHPNYLGHLTLGTTPETLKALEAADLVFVVG
                     CRLSEVTTQSYSLPRKDQAVIQVDIDPAEVGATVPIEIGLISDAEATLAALLDRAPIP
                     PTARDWTDAHGAYLDSSTIPPAHPSDAIHPAQVVGALRDALPADTLMTNDAGNFSIFL
                     HRYWHYNHAATQLASTNGAMGYGVPAAVAAKLAAPDRTVVASCGDGGFLMTGHEIETA
                     VRYGAAITVVVFRNGIHGTIAMHQKRDLGRTAGIEIGEVDLAGYARSLGAEGYTVRDP
                     EELVPALEAALTSDTVSLLDVVTDPDIISPSARLSELAARDS"
     gene            2546..3487
                     /locus_tag="VHM69_11840"
     CDS             2546..3487
                     /locus_tag="VHM69_11840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2741130.1"
                     /translation="MVVVGCGVIGLTAAVCLREAGFDARIVTAELPPATTSSVAAAVW
                     YPYRAYPEDRVLSWGSRTFEVFEDLAQDPTSGVIMREGVEIWRKRVPDPWWAGAVPGV
                     RRCREDELPPGYLDGHAFVAPIVEMPIYLKYLMDRFIAAGGNVVQHTLSSLDEVDARV
                     VVNCVGLGARELVGDTSMQPIRGQVVRVSNPGLQSFVLDEENPEGVTYIVPRSQDCIL
                     GGTAGEGEWDLEPDPETASCILRRCTELEPRLAGVEELEHRVGLRPGRPEIRLEREVV
                     AGGVTRIHNYGHGGSGITLSWGCAEETLSLVEVGPGA"
     gene            complement(3484..3936)
                     /locus_tag="VHM69_11845"
     CDS             complement(3484..3936)
                     /locus_tag="VHM69_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741131.1"
                     /translation="MRTIEVERAGSERRRSAVAEIREHLARLYRGFVAWASLYGEVDD
                     HDERERRERVVGLLDELSNQYLPRSVWLTEGSRKKIENFVQRAEQLCSEFSAEIEERG
                     YPRVRRSMEKRVSKKLRPLKSEAESGLEAELAEPRRPGWRERLRRREV"
     gene            complement(4135..>4555)
                     /locus_tag="VHM69_11850"
     CDS             complement(4135..>4555)
                     /locus_tag="VHM69_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2741132.1"
                     /translation="ASKVIPLEAGDSVSFSKTVGESDVYLFAGITGDFSPNHINEEIM
                     SKTPFEHRVAHGVLSLGFASTTSTMMIERSNMTAVSYGYDRVRFINPVLVGDTITATY
                     TIAEIDEETLKTYADVEIVNQRGELCTVARHILKFVM"
BASE COUNT          795 a         1433 c         1488 g          839 t
ORIGIN      
        1 tgctatggtg aggtagatct catggagagg aatgacatgg acatgagtgg catggggacg
       61 aacctggcgt cattacgaga gaggcggatg tggacccagg ttcggctcgc cgaggaggct
      121 ggggtcagtc cgacgacggt atccgggatc gagaacggga ggatctcgcg tccccacttc
      181 gggacgttgc gcaagctggc tgtggcgttg agggtggagc ctgaggagtt gctgccgcgt
      241 cccggcgtcg ggcgccacga gaatctctcg ctggagtggt ccatggcgtc cgatgacgag
      301 gagttcgagc gggggttgga ggacgcttcc atcgaggggc tctcctcgct ctccgacgag
      361 ttagatgagg agaaagtccg cttgcgcagg ctctatggag aggccagggg cgatgtgcag
      421 cggcgtctga taaagggcat gatccggcag gtagccgcca actccggctc cgtcgaagcc
      481 tccatcatgg cccaccccga taatgaggac gtggatgagg acgcggtccg ccacgagtag
      541 tcatcaggtt acgtcaattc tcaaaatgat agcccccggc atataagcag cctccaccag
      601 gcaacatcta aagacccgaa agcgaggccg caggccaatg cgagctgaaa ggcgccaaag
      661 gcgccgtgct gacaagcgaa ggcgaagcct cagcgtgctg aaagcgaagc ccgcagggcg
      721 tagcgtgctg acaagcgcca agggcagaag tcaggacttc gcttcaggag tctcttgccg
      781 cgagttcgga gagccgggct gaggggctga tgatatccgg gtctgtgact acgtccagca
      841 gggaaacggt atccgaagtg agtgcggcct ccagcgcggg tacaagctcc tcggggtcgc
      901 gcacggtgta accttccgcg cccaggctgc gggcgtagcc ggcgaggtcg acctccccta
      961 tctcgatgcc ggctgtgcgg ccgagatctc gcttctggtg catcgctatg gtgccgtgta
     1021 tcccgttacg gaacacgacg acggtgatag cggcgccgta gcggactgcc gtctctatct
     1081 cgtggcccgt catcaggaac ccgccatcgc cgcagctggc cacgactgtc cgatccgggg
     1141 cggcgagctt cgccgcgacc gcggcgggaa cgccgtagcc cattgctccg ttcgtcgagg
     1201 ccagttgcgt ggccgcatgg ttatagtgcc agtaacggtg gaggaagatc gagaagttcc
     1261 ctgcgtcgtt ggtcatcagc gtatccgcgg gcagagcatc gcgcagcgcg ccgacgacct
     1321 gcgcagggtg gatagcgtcc gaaggatggg ctggcggtat cgtgctcgag tccaggtagg
     1381 ctccgtgggc gtcggtccag tcgcgtgccg tgggtgggat cggggcccga tcgaggagcg
     1441 cggcgagggt ggcttcggcg tccgagatca ggccgatctc tatggggact gtggcgccca
     1501 cctctgcggg gtctatgtct acctggatca cggcctggtc cttgcgtggg agcgagtagc
     1561 tctgggtcgt cacctcgctc agacggcagc cgaccacgaa gacgaggtcc gccgcttcga
     1621 gggccttcaa ggtctccggc gtcgtaccga gggtgaggtg tccgaggtag ttcggatgct
     1681 cgttcgggaa gacgtcctgc ctgcggaacg atgcgtagac gccgatgtcg aacgcctccg
     1741 cgaaagagat caaagtctcc cgcgcgttcc gcgctccgcc accggcgatc acgaccggac
     1801 ggtgagcggc tgagagcctg cgggctacgg ccctgacctg atcggaagac ggtacgggac
     1861 ggggcggctc tggcaccctc gcgtccgggc cgggctgcac ctgttcgccg agcacgtcgg
     1921 ccgggagggc gagcatgacc ggcccgggac ggcccgaggt ggcgatgcga agtcccctcg
     1981 cgacgaactc ggcgaggcgg tcggcgcggt ggacggtggc ggcccacttc gtgatcgggg
     2041 cgtagaaagc ggtgaggtcc acctcctgaa acgcctctct cccgaggaag tccgtctcta
     2101 cctgcccaag caagacgagc atgggcgtcg aatcctgcct cgctgtgtgt acccctatgg
     2161 cgaggttcga cgcgccgacc ccccttgtcg ccatcgccac agccggaatc cccgtcagct
     2221 tgccttcagc ctcggccata aaggcggcgc ccgactcgtg gcgggtcgag atcagaccca
     2281 ggtccgggtg ctcctcggct gcgtccagga tctccaggaa gctttcgccg ggaacggtgt
     2341 atagtcgtct gactccggcc cctgccagaa cctccgccgc gacgtaaccc gctttcttcg
     2401 tgctcagatc cgccatcttt cccccgtctt ccgaaggtcc ttgcccatcg ggaccacctc
     2461 cgtacatcct acacgctcgg gtcgcggaag cgcccgaccg ggcatatagt gtgtctgacg
     2521 aggaacggag gggattctgg ctcgcgtggt tgtggtggga tgcggtgtga tcgggctgac
     2581 ggcggccgtc tgtctgcggg aggccggctt cgacgccagg atcgtgactg ccgaactgcc
     2641 gcccgccacc acctccagcg tggccgcagc cgtctggtac ccgtacagag cctatcctga
     2701 ggaccgcgtg ctctcctggg gcagcaggac gttcgaggtc tttgaggacc tcgcccagga
     2761 tccaacgagc ggcgtcatca tgcgtgaggg ggtggagatc tggcgcaaac gggtccccga
     2821 tccctggtgg gccggcgccg tgcctggagt gcgccgatgt agagaggacg agctacctcc
     2881 aggctacctg gacggccacg ctttcgtcgc accgatcgtc gagatgccca tctatctgaa
     2941 gtacctgatg gaccgcttta tcgccgccgg gggaaacgta gtgcagcaca ccctctcctc
     3001 actcgacgag gtggatgctc gcgtggtcgt caactgcgtc gggctcggcg cgcgcgagct
     3061 cgtcggagac acttcgatgc agccgatccg gggccaggtc gtacgcgtaa gcaaccccgg
     3121 ccttcagagt ttcgtcctcg acgaagagaa ccccgagggc gtcacttaca tcgttccccg
     3181 ctcgcaagac tgcatcctgg gcgggacggc tggcgagggc gagtgggacc tcgaacccga
     3241 tcccgagacc gcgtcctgca tccttcgccg ctgcacggag ctggagccgc gtctggccgg
     3301 cgtcgaggag ctggagcaca gggtaggact cagaccgggc cgcccggaga tacgcctcga
     3361 acgcgaagtc gtagctggcg gcgtcacccg catccacaac tacggtcacg gaggctcggg
     3421 catcaccctc tcctggggtt gcgccgagga gacgctcagt ctcgttgaag tagggcccgg
     3481 cgcctaaacc tcacgtctcc gcagacgctc tcgccacccg gggcggcggg gctcggccag
     3541 ctcggcttcg aggcccgact cggcctccga tttcagcggc cttagcttct tgctcacgcg
     3601 cttctccata ctccgtctga cccgcgggta gccccgctct tctatctcag cggagaactc
     3661 cgaacagagc tgttccgccc tctggacgaa gttctcgatc ttctttcggc tcccttcggt
     3721 gagccacacg gaccgcggca ggtactggtt ggatagctcg tcgagaagcc cgacgaccct
     3781 ctccctgcgc tcccgctcgt cgtggtcatc cacctccccg tacagggaag cccacgccac
     3841 gaaaccacgg tagagcctcg cgagatgctc ccttatctcc gctaccgccg atctacggcg
     3901 ttcgctcccc gccctctcga cctctatcgt cctcaatact gaatccttcc agcatgtact
     3961 cctctatggc acacggcgcc cacaacgggt tgtggaccag tcgcacaaca tagcacaaca
     4021 tgcagagttt ttcgtgcctg agggcgtccc tcagtcagac tcgttcacac ctactacgaa
     4081 ctccgcaacg gaagtagcgg aacgcggatt cgaaccgaac gtaccatgac ccttctacat
     4141 tacgaacttc aggatgtggc gtgcaacggt gcacaactct ccgcgctggt tgacgatctc
     4201 gacgtccgcg taggttttca gcgtttcttc gtcgatctcg gcgattgtgt aggttgccgt
     4261 gatcgtgtcc cccaccagta ccggattgat gaagcggacg cggtcgtatc cgtacgacac
     4321 ggcggtcata ttcgatcgct cgatcatcat cgttgaagtg gtggacgcga agcccagaga
     4381 taggacgccg tgggcgacac ggtgctcgaa gggagtcttg ctcatgatct cttcgttgat
     4441 gtggttgggg ctgaaatcgc ccgtgatgcc tgcgaataaa tagacgtcgg actcgccaac
     4501 cgtcttgctg aacgagactg aatcgccggc ttcgagcggg atgaccttgc tcgcc
//
LOCUS       DATAIR010000494         5060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_401404, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000494 DATAIR010000000
VERSION     DATAIR010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401404"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VHM69_11855"
     CDS             <1..825
                     /locus_tag="VHM69_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2741133.1"
                     /translation="VGSAVTNVEVGDTVIMHPLMTCGLCMNCRAGDDMRCEFGEFPGL
                     SRDGGFADLLKTNARAVVKLDPILEPKDIAALADAGLTAYHAVRKSVPLLNAGTRAVV
                     IGAGGLGHIGIQCLKAMTPTEVIVVDPSEEALSLAKEWGADQTVQVAEGYLDTVLELT
                     DGKGAEVVFDYVGERSAEDDAWQMTREGGSHYVIGYGGTIKVPTIDIISTERNIIGNL
                     VGTYTDLAELMVLTAQGKVSLHTSTYPLDAVNDAMQDLNNGNLRGRGILIPDGANA"
     gene            complement(865..1827)
                     /locus_tag="VHM69_11860"
     CDS             complement(865..1827)
                     /locus_tag="VHM69_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2741134.1"
                     /translation="MLDNLCDVPGVLVGHATDREGITGCTAVLFDGPEGAVVGVDVRG
                     SSPGTRETDRLGPVGAVRPTHALLLTGGSAFGLSAADGVVRHLEEKGIGLDVGVARIP
                     LVSAAVLFDLVVGDPASRPDAAMGYEAATSATSGDFEQGTVGAGTGASVGKVLGMERA
                     MKGGIGSASVELQDGLVVGALAAVNAFGEVRDPRTGKVLAGPRMEDGTPGDTVELLYE
                     AADRLRWGENTTLGIVATNARLSKPQVTKVAQMAHDGLARAVYPVHTTVDGDVAFAAS
                     TGGVEVAPDVVGAWGARVMQEAIVRAVLAAEGIADLPSARDLKV"
     gene            complement(1834..2694)
                     /gene="ricT"
                     /locus_tag="VHM69_11865"
     CDS             complement(1834..2694)
                     /gene="ricT"
                     /locus_tag="VHM69_11865"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX2741135.1"
                     /translation="MDVKVPGWSITRLCYARDLDLRVGYTVVVATEVGEFVGRVALTP
                     RRREAYTEPYDILRIVTEEDSRAEDRNRELGRRIREEARKLARDHRIEGVQFIGCDVS
                     LDGSYIEVKYQTEEKDLDLGPVKSGLQRAYDAQVVLREFRFIERSGDASGCDTCGLPL
                     CCATWSGARSMGPVNVRLAKQQGVTPNEKILGCCGEVKCCMRYEHDTYKEFKERAPFR
                     NSTVKLGRREGRVVDYSMVKDSVLVQFGSKRADQELVSLGSLARDNPGIIPADTDEWE
                     VPEPPQRPDS"
     gene            complement(2613..3776)
                     /locus_tag="VHM69_11870"
     CDS             complement(2613..3776)
                     /locus_tag="VHM69_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741136.1"
                     /translation="MAEQRDRLDGILGNETALRLLGRALRTGEVSQAYLFHGPPGVGK
                     RTVARRFGAALVAGGDMAAEDRARRGMHPDLAEILPEGAFTTIGQVREIVRLAASRPF
                     EGERRAFVLQADTLNVQAANALLKTLEEPEGETVFVLLATSREGVLPTVLSRAQAVRF
                     SPVPTSIVARFLEGRGAAEPGLAAALGRGSVGLSLRYAEDPEFGELREAVFEAGFAFS
                     EDFEARHGAVRRIVGRAESVGEAREREVIARFEEPDRRAKDAAKRAGRAARDGAVREA
                     LELLALLYRDTAVVQTGAEDLVANADRVGEIRGRLDEYPWADWAGAATAIGEARAALA
                     YNVSPEAILEVTLSRIRRKILGPSPGSWTSRSLAGASPGSVTRGTWTSGSATR"
     gene            complement(3769..4548)
                     /gene="tmk"
                     /locus_tag="VHM69_11875"
     CDS             complement(3769..4548)
                     /gene="tmk"
                     /locus_tag="VHM69_11875"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX2741137.1"
                     /translation="MARGGDARGRCAAGQPLRGARVRVGASRPPQGRGLARRRAAQTP
                     QDTRRGIFVTIEGPDFSGKSTLVRAIRPLVESMDLPTHFTREPGGTAAAERIREVVLD
                     PDLEMDAWTEAYLYAAARADHVRGEILPRLEAGENVLCERYLDSSLAYQGFGRGLGVE
                     AVRSLNAYAVGTVVPDRSFYLRIDHDERERRARELGAPPDRIEALGAGFMRRVEEGFE
                     ELARIEPERIEVLDASLSPRELAETVVGRMRALQYTRWSDG"
     gene            complement(4443..>5060)
                     /locus_tag="VHM69_11880"
     CDS             complement(4443..>5060)
                     /locus_tag="VHM69_11880"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2741138.1"
                     /translation="VGDERAAEAVIHGLDGLLLSGGSDLDPTYYGEEPLPELGVTLPE
                     RDAFEMALVDLALRRGMPVFGICRGMQVLNVALGGTLYQDLPSQWEGGVLKHRQDTPK
                     WQPTHEVMVGEGSYLGEVMGSELVKVNSYHHQGVKDLADSLVVTGRSSDGVIEAVEAG
                     DFTERWLLGVQWHAEAMRGAGVQQVSLFEAHVSASERHALRRAAA"
BASE COUNT          866 a         1823 c         1507 g          864 t
ORIGIN      
        1 gtgggctccg cagtcaccaa cgtcgaggtc ggggacacgg tgatcatgca ccccctgatg
       61 acctgcggcc tgtgtatgaa ctgccgcgct ggcgacgaca tgcgctgcga gttcggcgag
      121 ttccctggcc tctcccgcga cggcggcttc gccgacctcc tgaagaccaa cgcccgcgcg
      181 gtcgtgaagc tagacccgat cctggagccg aaagacattg cggccctcgc cgacgccggc
      241 ctcaccgcat accacgccgt caggaagtcc gttccgttgc tcaatgcggg cacgagggcg
      301 gtcgtgatcg gggcgggagg tctcggccac atcggcatcc agtgcctgaa ggcgatgacc
      361 ccgacggagg taatagtcgt cgacccgagc gaggaggcgc tcagtctggc gaaagaatgg
      421 ggagcagatc agacagtgca ggtcgcggaa ggctacctgg acacggtact cgagctgacg
      481 gacgggaagg gcgcggaggt cgtcttcgac tacgtcggcg agcgaagcgc agaggacgac
      541 gcctggcaga tgaccaggga aggcggctcg cactacgtca tcggctacgg tggcacgatt
      601 aaggtaccaa ccatcgacat catctccacg gagcgcaaca tcatcgggaa cctcgtcgga
      661 acatatacgg acctcgccga gctgatggtc ctcacggccc aggggaaggt cagcctccac
      721 acctcgactt atccactcga cgccgtcaac gacgccatgc aggacctcaa caacggcaac
      781 ctccgtggtc gcggaatcct gatccctgac ggagcgaacg cctgaattac tgacattaag
      841 ccgcaatgag gctatggtcc gggattagac cttgagatcc ctcgccgagg gtaagtcggc
      901 gatgccttca gcggcgagca cggcccgcac gattgcctcc tgcatgaccc gcgcccccca
      961 cgctcctacg acgtccggcg ctacctcaac cccacctgtt gacgcggcga aggctacgtc
     1021 gccgtccacg gtcgtgtgga cgggatagac ggccctggcc aggccgtcgt gggccatctg
     1081 ggcgaccttc gtaacctggg gcttcgagag cctcgcgttg gtcgccacta taccaagggt
     1141 ggtgttctcg ccccacctga ggcggtccgc ggcctcgtag agcagctcga cggtatctcc
     1201 cggcgtgccg tcttccatgc gcggccctgc gagcaccttc ccggtccgag ggtccctgac
     1261 ctcgccgaaa gcgttcacgg cggccaatgc tccgacgaca agcccgtctt gcagttcgac
     1321 ggaagcgctc ccgatccccc ccttcatcgc ccgctccatg ccgagtactt ttcccaccga
     1381 ggctccggtc ccggctccga cggtgccctg ctcgaaatcg ccgctcgtgg ccgaagtggc
     1441 ggcctcgtag cccatcgccg cgtcggggcg ggaggcggga tcccccacca cgaggtcgaa
     1501 gagaacggcc gccgagacga ggggaatgcg tgccacgccg acgtcgaggc cgatgccctt
     1561 ctcttcgagg tggcggacga caccgtctgc ggcggagagc ccaaaggcgc ttccacccgt
     1621 caggagcagg gcgtgcgttg gacgcaccgc gcccaccggt ccgaggcggt cggtctcccg
     1681 cgtccctggt gatgagccgc gcacgtccac gccgactacg gcgccctcgg ggccgtcgaa
     1741 gaggacggcg gtgcagcctg tgatcccctc gcggtcggtc gcgtggccga ctagcacacc
     1801 aggcacgtcg cagaggttat ctaacacgtc ggcctaggag tcagggcgtt gcgggggttc
     1861 gggtacctcc cactcatccg tgtcagcggg gatgatccct gggttatccc tggcgagtga
     1921 accgagcgaa actagttctt gatccgcgcg cttggagccg aactgaacga ggaccgagtc
     1981 cttgaccatg gagtaatcaa cgaccctgcc ctctcgcctc ccgagcttca cggtcgagtt
     2041 gcggaatggc gcccgctcct tgaactcttt gtacgtgtcg tgctcgtagc gcatacagca
     2101 cttgacctcg ccgcagcagc ccaggatctt ctcgttcggc gtaacgccct gctgcttggc
     2161 gagcctgacg ttgacgggtc ccatgctcct cgctccgctc cacgtcgcgc agcagagagg
     2221 cagcccgcac gtgtcgcacc cgctcgcgtc accagaccgc tctatgaagc ggaactcccg
     2281 caagacgacc tgagcatcgt aggcccgctg cagtccgctc ttgaccgggc caaggtccag
     2341 gtccttctct tcggtctgat atttgacctc gatgtaggag ccgtctaatg acacgtcgca
     2401 ccctatgaac tgcacgcctt cgatgcggtg gtccctggca agctttctgg cctcttccct
     2461 gatacggcgc cccagttccc tgttgcggtc ttccgccctg gagtcctcct cggtgacgat
     2521 cctgagtatg tcgtagggct cggtgtacgc ctcgcgacga cgtggcgtca gggcgacacg
     2581 cccgacgaac tcccccacct cggtcgcgac gactaccgtg tagccgaccc tgaggtccag
     2641 gtcccgcgcg taacagagcc gggtgatgct ccagccaggg accttgacgt ccacgagcct
     2701 ggggagggtc cgagtatctt ccgccgtatg cgcgataacg tcacctccag gatggcctcc
     2761 ggagagacat tatacgcaag tgccgcgcgc gcttccccta ttgccgtcgc agcgccggcc
     2821 cagtcagccc aaggatactc gtccaggcgc cctcgtatct cccccaccct gtccgcgttg
     2881 gcgaccaggt cctcagcacc tgtctggacc accgctgtat ccctgtatag gagggcgagc
     2941 aactcgagtg cctcgcgaac tgcgccgtcc ctcgccgccc tgcccgcgcg tttggccgcg
     3001 tccttcgccc gacggtccgg ctcctcgaac cgggcgatga cctcacgctc ccgcgcctct
     3061 cccacggact cggcgcggcc aacgatccgc ctgacggccc cgtgacgtgc ctcgaagtcc
     3121 tccgagaagg cgaacccggc ctcgaagacg gcctctctga gctccccgaa ctcggggtcc
     3181 tccgcatacc gcaacgagag cccgacgctc ccccttccga gcgccgccgc gagcccgggc
     3241 tccgccgcgc ctctgccttc aaggaaccgt gcgacgatgc tcgtcggcac ggggctgaac
     3301 cgcaccgcct gagctcggga aaggaccgtg ggcagcaccc cctcgcgcga ggtcgcgagc
     3361 aacacgaaaa ccgtctcccc ctccggctcc tccagcgtct tgagcagcgc gttcgcggcc
     3421 tggacgttca gcgtgtcagc ctgcaagaca aacgccctgc gttcaccttc aaatggcctg
     3481 ctggccgcga gacgtactat ctccctcacc tgcccgatag tagtgaaagc accttcgggc
     3541 agtatctccg cgaggtcagg gtgcataccc ctgcgtgccc tgtcttccgc cgccatgtcg
     3601 cctccagcga ccagcgctgc cccgaacctc cgggcgaccg tccgcttgcc tacgccggga
     3661 ggaccgtgga acagatacgc ctgcgagact tcgcccgtcc gcagagcccg cccgagcagc
     3721 cgcaacgccg tctcgttccc cagaatgcca tcgagcctgt cccgctgttc agccatcact
     3781 ccagcgagta tattgcagcg cacgcatcct acccaccacc gtttcggcga gctcccgcgg
     3841 tgaaagcgaa gcgtcgagga cttcgatcct ctccggctca atccgcgcca gttcctcgaa
     3901 accctcctcg accctccgca tgaagccagc gcccaacgct tcgatcctgt ccggaggcgc
     3961 acccagctcc ctcgcacgcc gctccctctc atcatgatcg atccgcaggt agaaagaccg
     4021 atcaggcacc acggtcccga cagcataggc gttcagagat cgtaccgcct cgacgccgag
     4081 tcccctcccg aaaccctggt aggcgaggct ggagtccaga taccgttcgc agagcacgtt
     4141 ctcgccggct tcgaggcgcg ggaggatctc tcctctgacg tggtccgcgc gggccgcagc
     4201 atacaggtac gcctccgtcc aggcgtccat ctccaggtcc ggatccagga ccacctccct
     4261 gatgcgctcg gccgcagcgg tacctccggg ctcgcgggtg aagtgggtcg gaaggtccat
     4321 gctctcgacg agaggcctga tggcacgcac cagtgtggac ttgcccgaga agtcaggacc
     4381 ctcgatcgtt acgaaaatgc ccctgcgcgt atcttgcggg gtctgcgcgg cgcgccttct
     4441 agctaggccg cggccctgcg gagggcgtga cgctccgacg cggacacgtg cgcctcgaag
     4501 aggctgacct gctgcacacc tgccccgcgc atcgcctccg cgtgccactg gacgccgagc
     4561 agccacctct cagtgaagtc ccccgcctcg acggcctcaa tcaccccgtc cgaggagcgg
     4621 cccgtcacga caaggctgtc tgcgaggtct ttgaccccct gatgatggta agagttgacc
     4681 ttgacgagct cgctgcccat aacctcaccg aggtaggatc cttctcctac cattacctcg
     4741 tgtgtcggtt gccacttggg ggtgtcctgc ctgtgcttga gcacgcctcc ttcccactgc
     4801 gaaggaaggt cctgatacag tgtcccaccc aaggcgacgt tgaggacctg catcccgcgg
     4861 cagatgccga agacaggcat tccgcgccgc agcgccagat ccacgagcgc catctcaaaa
     4921 gcatcccgct ccgggagcgt cacacccagc tcgggcaagg gctcctcacc atagtaggtt
     4981 gggtccaggt cgctcccgcc gctcaatagc aggccgtcga gcccgtgaat gaccgcctca
     5041 gcagccctct cgtccccgac
//
LOCUS       DATAIR010000495         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_309239, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000495 DATAIR010000000
VERSION     DATAIR010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309239"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="hypD"
                     /locus_tag="VHM69_11885"
     CDS             complement(<1..844)
                     /gene="hypD"
                     /locus_tag="VHM69_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX2741139.1"
                     /translation="MKPDRPKGATIKYVDEFRNAEIARAVSAEIAAITDPQRHYKLME
                     VCGGHTHTIYRHGIKDLLPDQIELVHGPGCPVCVLPMGRVDDGIAVAERPEVIFTCFG
                     DMMRVPGSKGNLLEIKAEGADIRMVYSPLDALRIARENPDREIVFFAIGFETTAPSTA
                     LTLQRAKKEGLENFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEF
                     MPEDYGKPCVVAGFEPLDLLQSILMLMRQLDEGRCEVENQYSRIVPWEGNLAALRSMA
                     EVFEL"
     gene            complement(841..1068)
                     /locus_tag="VHM69_11890"
     CDS             complement(841..1068)
                     /locus_tag="VHM69_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2741140.1"
                     /translation="MLPDSHCTLDHDGCVVCSDAGIPLRVVSIEGDDALCEDAVGNRT
                     EIALELVQPVAVGEVLLTHGGVAIGKIEPDA"
     gene            complement(1080..1358)
                     /locus_tag="VHM69_11895"
     CDS             complement(1080..1358)
                     /locus_tag="VHM69_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2741141.1"
                     /translation="MCLGIPGRIVEILDEDLLLAKAEVSGVRRNINIGLVHNEDEQVE
                     VGDWVLIHVGFAMSRLNETEAQSQLQALEDIGEAYEQELAELRASQIE"
     gene            complement(1399..3735)
                     /gene="hypF"
                     /locus_tag="VHM69_11900"
     CDS             complement(1399..3735)
                     /gene="hypF"
                     /locus_tag="VHM69_11900"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX2741142.1"
                     /translation="MRIPTRERREVSVRGIVQGVGFRPFVYALARKHELSGMVRNDAE
                     GVRIEAEGSPEELELFVRALEDDAPPLAVVESVLWQPISPLGDREFRIAESREGLRRQ
                     ALVSPDVATCDDCLGELLDPEDRRYRYPFTNCTNCGPRFTITRQVPYDRATTTMSGFT
                     MCPDCQKEYDDPSDRRFHAQPNACPVCGPRVRLLDRFGHELHAKPEDPIARAARMLRG
                     RAVVAIKGLGGYHLACDPFDEKAVRTLRGRKVRQDKPFALMARDLAQIRELCHVSAED
                     EALLTSPAHPIVLLERRDDSGVVEEVAPRQNTLGVMLAYTPLHHLLLGDAGIPLVMTS
                     GNNSDEPIAYRDDEAFDQLCEIADYFLIHDREIHMRCDDTVVRTAGRHPYLIRRSRGY
                     APAPLRLAGIVTRHTLACGGELKNTFCLARGSHAFVSHHIGDMENYETLISFREGIEH
                     YCRLFDVTPELVAYDLHPEYLSTKYARDLEEAGLSAVGVQHHHAHVASCLADNEVSAE
                     ERSIGVALDGAGYGTDGAVWGGEFFEGSLEAGFTRRAHLEYAPLPGGSAAIKQPWRMA
                     LGYLITHYGEEETLKLPLAAVRNAGERNVHLIARLVEHNLNTPPTSSAGRLFDAVAAL
                     VGVPGSQRTTYEGQAAIELELAANGRTKQAYPFRLRPEHDAWIVETHEVIAAVFEDLL
                     RGCPTSEISASFHRTMAEMVAAVCEELRKADGASAVALSGGTFQNMMLLKQTIELLEE
                     KQFVVYTHRRVPANDGGIALGQAILADRAFDKDGVADG"
     gene            complement(3924..>4125)
                     /locus_tag="VHM69_11905"
     CDS             complement(3924..>4125)
                     /locus_tag="VHM69_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741143.1"
                     /translation="TLVVGCEPEVILSGEDYDDILMELSEPVRTAVEEAAKLVESLVT
                     EVSTENRPGRSAGTSTESEVKP"
BASE COUNT          707 a         1269 c         1282 g          867 t
ORIGIN      
        1 ggagctcgaa gacctcagcc atgctcctca gggctgcgag gttgccttcc cagggaacga
       61 tcctgctgta ctggttctcg acctcgcaac gcccttcatc gagctggcgc atgagcatga
      121 ggatggactg caaaaggtcg agcggctcga accccgccac tacacacggc tttccgtagt
      181 cctcgggcat gaactcgtat ggcctgcagc cgatcaccgt cgatacgtgt ccgggcccta
      241 taaaagcatc caggcgcagg tcaggggagt cgagcagcgc ccgcacaggc ggcacgatgg
      301 tgacgtggtt gcagaatacg gagaagttct ccaggccctc cttctttgcc cgctgcagag
      361 tcagggccgt cgatggggcc gtggtctcga agcctatggc gaagaaaact atctcgcggt
      421 cagggttctc tctcgctatg cggagggcgt ccagaggcga gtagaccatg cggatgtcag
      481 cgccttcggc cttgatttcg agcaggttcc ctttcgagcc agggacacgc atcatgtcgc
      541 cgaagcaggt gaatatgacc tcggggcgct cggcgacggc tatgccgtcg tccacgcggc
      601 ccatcggcag gacacaaaca gggcaaccag ggccgtggac aagctctatc tggtccggta
      661 agaggtcttt tatcccgtgg cggtagatgg tgtgggtgtg gccgccgcag acctccatca
      721 gtttgtagtg tctctggggg tcggtgatcg cggctatctc agccgatacg gcgcgtgcga
      781 tctcagcgtt tctgaactcg tctacgtatt tgatggtcgc tcccttcggt cgatcaggtt
      841 tcaggcatca ggttcgattt tcccgatggc gacgcctccg tgcgtgagca gcacttctcc
      901 cacggctacc ggctggacca gttccaatgc gatctcggtc cggtttccga cggcgtcttc
      961 gcagagggcg tcgtcgcctt cgatcgagac gaccctaagg gggatgccgg cgtcggagca
     1021 gacgacgcaa ccgtcgtggt cgagggtgca gtgtgagtca gggagcatct tttccacggc
     1081 tactctatct gggaggcgcg cagctccgcc agctcctgct cgtaggcctc gcctatatct
     1141 tcgagggcct gaagctggga ctgcgcctcg gtctcgttca accgcgacat cgcgaagccg
     1201 acgtggatca ggacccagtc tccgacttcg acctgctcgt cctcgttgtg gacaagccct
     1261 atgttgatgt tgcgtctgac tccgctcacc tcggcctttg cgagcagaag gtcctcatcc
     1321 aagatctcta ctatcctgcc aggtattcca aggcacatgg gttgcctccc ttcggtcggc
     1381 ggcttcaggc ttcaggtatc agccgtcagc tactccgtcc ttatcgaatg ctctgtccgc
     1441 cagtattgcc tgtcccaggg cgattccgcc gtcgttggca gggacacgcc tgtgtgtgta
     1501 gaccacgaat tgcttctcct ctagtagttc tattgtctgt tttagtagca tcatattctg
     1561 gaaggtgccg cctgagaggg cgacggcgct cgctccatct gccttgcgga gctcttcgca
     1621 gaccgcggcg accatttcgg ccatcgtgcg gtggaagctg gctgagatct ctgatgtcgg
     1681 acatcctctc agcaggtctt cgaatactgc ggctatgact tcgtgggtct cgactatcca
     1741 ggcgtcgtgc tccggtctca ggcggaaggg atacgcttgt ttcgtccgtc cgttcgcggc
     1801 cagttccagc tctatggcgg cctgcccttc gtaggtcgtg cgttgagatc caggtacgcc
     1861 gacgagggcg gcaacggcgt cgaagagacg gcccgcgctc gaggtcggcg gcgtgttcag
     1921 gttgtgctcg acgaggcggg cgatgaggtg tacgttcctt tcaccggcat tgcgcacggc
     1981 ggcgaggggg agcttcaggg tctcctcttc tccgtagtgt gtgatgaggt acccgagcgc
     2041 catgcgccag ggttgcttga tagccgccga tccccctggc agtggagcat attccaggtg
     2101 agcccgacgt gtgaaccccg cttctagact gccctcgaag aactctccgc cccagacggc
     2161 cccatccgtc ccgtagcccg ctccatcgag ggcgaccccg atggaacgct cctccgccga
     2221 cacctcgttg tcggcgaggc agctcgcgac gtgggcatgg tggtgctgga ctccgaccgc
     2281 ggaaagcccg gcttcttcga gatcgcgggc gtatttggtc gataggtact cgggatggag
     2341 gtcgtaggcc accagttccg gggtaacgtc gaagagcctg cagtagtgtt cgatgccctc
     2401 cctgaaagag atcagcgtct cgtagttctc catgtccccg atgtgatggc tgacgaaggc
     2461 gtggctgccc ctggccaggc agaaggtgtt cttgagctcc ccgccgcagg caagcgtgtg
     2521 gcgggtgacg attccggcca gtcggagcgg cgcgggggcg tagcctcggg agcgacggat
     2581 caggtacggg tgtcttccgg ctgtccggac tacggtgtcg tcgcagcgca tatggatctc
     2641 acggtcgtgg atcaggaagt agtcggcgat ctcacacagc tgatcgaagg cctcatcgtc
     2701 cctgtacgct atgggctcat cagagttgtt gccgctggtc atgacgagag ggatgccggc
     2761 gtcacccagc agcaggtgat gtagcggcgt gtacgcgagc atcacgccca gagtgttctg
     2821 gcgcggggca acctcttcga cgacgccgga atcatcccga cgttcaagga gcacgatggg
     2881 atgggccggt gaggtgagca gtgcttcatc ttccgccgag acgtggcaaa gttctcgtat
     2941 ctgcgctagg tctcgggcca tgagggcgaa gggcttgtcc tgacgcacct tgcggccccg
     3001 cagggtcctc accgctttct cgtcgaacgg gtcgcaggcc agatgatagc caccgagccc
     3061 ctttatggcg acgactgccc tgcctcgcaa catacgggca gcccgcgcga tggggtcctc
     3121 cggcttggca tggagctcgt gtccgaacct gtcgagcagc cgtacccgtg gcccgcagac
     3181 ggggcatgcg ttaggctggg cgtggaagcg tcggtccgac ggatcgtcgt actccttctg
     3241 gcaatccggg cacatcgtaa agcctgacat cgtggtcgtc gccctgtcgt acgggacctg
     3301 gcgggtgatg gtgaagcgcg ggccacagtt cgtgcagtta gtaaacgggt aacggtagcg
     3361 ccggtcttca ggatccaaca actcaccgag gcagtcgtcg caggtcgcga cgtcagggga
     3421 aacgagcgcc tgccgacgga gcccctctcg gctctcggcg atgcggaact ccctgtcccc
     3481 gagcggcgag atcggctgcc agagaacgga ctcgaccacg gccaggggag gggcatcatc
     3541 ctcgagagcg cgcacgaaga gttcaagttc ctcaggtgag ccctcagcct cgatgcggac
     3601 accctccgcg tcgttgcgga ccatccccga gagttcgtgc ttgcgcgcca gggcgtagac
     3661 gaacggcctg aagccaaccc cctgcacgat gccgcgtacg gagacttcgc gacgctcacg
     3721 ggtagggatt ctcacgatgt cagccttcgc agacgcacga gagtcttcat cgccttgcta
     3781 ctcaacgatt tgctcgaagt ggctcgcagt tcgtactccc tcaagctaca tattgcgcat
     3841 ctttatatag cgttggatct ccggccactg tttggccaca gctaccccgg cccccagaac
     3901 caatccaagg aacaagagct tcctcatggc ttcacctcac tttccgtgga agtcccggct
     3961 gatctaccgg gtctattctc tgtgcttacc tccgtaacta gcgactccac cagcttcgcg
     4021 gcctcctcta ccgccgtacg taccggctcg ctcagttcca tcagtatgtc gtcgtagtct
     4081 tctccgctca ggatcacctc aggctcgcat ccgaccacga gggtt
//
LOCUS       DATAIR010000496         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_60712, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000496 DATAIR010000000
VERSION     DATAIR010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60712"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VHM69_11910"
     CDS             <1..1272
                     /locus_tag="VHM69_11910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2741144.1"
                     /translation="PQTVILKYGAGVRSIVGGASSAVPGSLRGWEKILERHGRLGLKE
                     VFAPAVRFAREGFRLCKTSALWLQVAEEVLNLTGETRKHFYDGDRVYLEGEVMRFPEL
                     ADTLQALGEDGARLFYEGELARSISTYMLKMGGIITEEDLAQYEAVVREPMSVRYGAG
                     EMYTNGPPSAGGSTLAQMLKVVSAYDLATMPEAEYVRIMAGAMKLALQDRETAYLDGS
                     ENGRVAERLTGEEYAARQRRRILSGFGSPHTTHLSCVDGEGLAVSITASMGYGSGLVV
                     PGTGIPINNTLGEPELNPKGFHALKPGERLISSMSPTVVSSEDGGLIALGSPGASRIP
                     TAILQTVINVLDFGMSLEDAVLAPRFHAEGDLFAYEAGARTADLDLYERVLSYETLNM
                     FFGGVNAVRQTPEGRFEAAADPRRSGGTAHV"
     gene            1265..2188
                     /gene="rocF"
                     /locus_tag="VHM69_11915"
     CDS             1265..2188
                     /gene="rocF"
                     /locus_tag="VHM69_11915"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX2741145.1"
                     /translation="MSENARRPVGIFGVPMDLGQDRRGVDMGPSAIRYARLEASLTDL
                     GHKVTDLGNAGVPIPELVAKEEVVKHLAAVESVCESVSERAATIFSEGLFPIFLGGDH
                     SIAIGTVSGVARSMSGDRTGVIWVDAHADFNTPATSPSGNIHGMPLATLTGRGHPDLV
                     GIGGPGASVSTEDVVIVGLRSVDLEEKNLLREAGVKVYTMKEIDAYGVARVVRSAMND
                     LSHVDKVHLSLDLDVVDPEVAPGVGTPVRGGLTYREAHLLMELVNEAGIVSSLDVVEV
                     NPILDVKNGTATLAVELVESLMGRRIIDLPR"
     gene            complement(2347..3810)
                     /locus_tag="VHM69_11920"
     CDS             complement(2347..3810)
                     /locus_tag="VHM69_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX2741146.1"
                     /translation="MNPRVIIRVNAGVILAIGLSLLIPLALSLLYEDGSWWSFLLPAA
                     VMVAAGALGFRAARPRGRSPEYVSNRDVYLSVTLAWTLAALLGGTPFLVEGTFHSLLD
                     SSFEAMSGFTTTGATLLSDIEAQTPSILFWRSMTHWLGGIGIVVLFVAVAPLLGVGAA
                     RLLGAEMSGLTQPRLTPRIADTAKALVVIYLIISIAETVALLLAGMPLYDSVVHMFGT
                     VATGGFSNKTASVGFYDSLAIEAIIGVFMVASGVSFSLYYLLYTRRRLSVMLDRELLA
                     YLGIIAAASLFVWGILVLEGDYGASWGLALRDSAFAVSSIITTTGYITADFDRWDTAA
                     QITLVLLMFIGGCAGSTAGGIKVIRILIVFRTILQDVFRMIHPRAVTPLQIGERVVPE
                     TVRIAVLGLFAAWIGVFGIATFIVALQEELTPLSSVTAVATTLNDVGPGLGQVGATES
                     FEIVNPLGRFVLTVCMLLGRLEIFTVLALLSPAFWRR"
BASE COUNT          786 a         1201 c         1270 g          639 t
ORIGIN      
        1 ccacagacgg ttatcctcaa gtacggcgcg ggcgtgcgtt cgatcgtcgg aggagcgtcc
       61 tcggccgtcc ccggatcgct ccgcggatgg gagaagattc tagagcggca cggccggctg
      121 gggctcaagg aggtgttcgc cccagcggtg aggtttgcgc gagaggggtt caggttgtgc
      181 aaaacgtcgg cgctctggtt gcaggtcgcc gaggaggtcc tcaacctgac aggcgagacc
      241 agaaagcact tctacgacgg ggacagggtg tacctggagg gcgaagtgat gcggttcccc
      301 gagctcgcag atacgctcca ggcacttggg gaggatgggg cgaggctctt ctacgaggga
      361 gagctggcgc gcagcatctc cacctacatg ctaaagatgg gcggcatcat aaccgaggag
      421 gacctcgcgc agtacgaggc cgtcgtcagg gagccgatga gcgtacgtta cggggccggg
      481 gaaatgtata caaacggacc gccctcggcc ggtggttcga cgctggccca gatgctcaaa
      541 gtcgtatccg cttacgacct tgcgaccatg cccgaggccg agtacgtgag gatcatggcc
      601 ggggcgatga agctggcgct ccaggaccgc gaaaccgcct acctcgacgg ttctgagaac
      661 ggacgtgttg ccgagcgtct gaccggcgag gagtacgcgg cccggcagcg gcgcaggatc
      721 ctctcgggct tcggatcgcc gcacacaacg catctctcgt gcgtagacgg cgaggggctt
      781 gcagtttcta tcacggcgag catggggtac gggtccgggc tcgtcgtccc aggcacgggc
      841 atcccgatca acaacaccct cggcgagcct gagctaaatc ccaagggctt tcacgcgctc
      901 aagcctgggg agcgtttgat ctcgagcatg agcccgaccg tcgtctcttc cgaggatggc
      961 ggcctcatag cgctgggatc tcccggcgca tcgcgcatac caacggccat tctgcagacc
     1021 gtcatcaacg tcctcgactt cgggatgtcg ctcgaggacg ccgtactcgc gccgcgtttc
     1081 cacgcggagg gtgatctctt cgcgtacgag gctggtgccc gaacggctga cctggatctc
     1141 tacgagcgcg tgctcagcta cgagaccctc aacatgttct tcggtggggt aaacgccgtc
     1201 aggcaaactc ctgaaggacg cttcgaggcg gccgcagatc cccggcgttc tggaggtact
     1261 gcccatgtct gagaatgccc gtaggccggt tggaatcttc ggtgtgccga tggacctcgg
     1321 ccaggaccga agaggcgtcg acatgggacc gagcgccatc cgatacgccc gtctcgaagc
     1381 gtcccttacg gacctcggcc acaaagtcac ggacctggga aacgccgggg tgcccatacc
     1441 cgagctggtc gccaaagaag aggtggtcaa gcacctcgcc gccgtcgaga gcgtctgtga
     1501 gtcggtgtcg gagcgcgcgg cgaccatatt ctcggaaggt ctgttcccga ttttcctcgg
     1561 cggggaccac tcaatcgcca tcgggaccgt ctccggggtg gcgcgctcca tgtctgggga
     1621 caggacgggt gttatctggg tggacgccca cgcggatttc aacacccctg cgacatcgcc
     1681 ttccggcaac atacacggca tgccgctggc gaccctcacg ggacgcggcc accccgacct
     1741 cgtcgggatc ggcggcccag gtgcgagcgt cagcaccgaa gacgtggtga tcgtcggcct
     1801 taggtccgtg gatctcgaag agaagaacct gttacgtgag gccggggtca aggtctatac
     1861 aatgaaggag atagacgcct acggcgtcgc ccgcgtcgtg cgaagcgcca tgaatgacct
     1921 ctcgcacgtg gacaaagtgc acctctctct cgacctggac gtcgtcgacc ccgaggtcgc
     1981 ccccggcgtc ggcacccccg tgcgaggtgg gttgacgtat cgggaggcgc atctcctcat
     2041 ggagctcgta aacgaggccg ggatagtctc ttcgctcgac gtcgtcgagg tcaacccgat
     2101 ccttgacgtc aagaacggca ccgccacgct cgccgtcgag ctggtcgaga gcctgatggg
     2161 gcggagaata atagacttgc ccaggtaggt ttcaggctac aggcatcagg attactagaa
     2221 cgcgagcagg ttcgcaacgg tgaccccgta cgccagtttc acgcttcgac ctaacagaac
     2281 catgcaggag cgagtgccgt cggccctcga caaaatccca aaacctgtag cctgaagcca
     2341 ctgttttcag cggcgccaga acgcgggcga gagaagcgcc agaaccgtaa agatctcgag
     2401 cctcccgagc agcatgcaga ccgtgagcac gaagcggccc aaagggttga cgatctcgaa
     2461 gctctccgtg gcaccgacct gcccgagccc aggaccgacg tcgttgaggg tcgtagccac
     2521 cgctgtgacc gaagagaggg gcgtcaattc ctcctggagc gccacgatga acgtcgcgat
     2581 gccgaagacc ccgatccagg ccgcgaagag cccaagcacc gcgatccgga cggtctcggg
     2641 cacgacgcgc tcgcctatct gaagtggcgt taccgcgcgc gggtgaatca tcctgaagac
     2701 gtcctggagg atcgtacgga aaacgatgag gatacggatg accttgatcc cacctgccgt
     2761 cgaaccggcg catccaccga taaacatgag caggaccagt gtgatctggg ctgccgtgtc
     2821 ccagcggtcg aagtcagccg taatgtagcc cgtcgtcgtg atgatgctgg ataccgcgaa
     2881 cgccgagtcc ctgagcgcca ggccccagga ggccccgtag tcgccttcca ggacgaggat
     2941 tccccacacg aacagcgatg ccgcagcgat gatcccgagg tacgctaaca attcacgatc
     3001 gagcatgacg gacagacgac ggcgtgtata cagcaggtag tacagcgaga acgagacgcc
     3061 cgaggccacc atgaagacgc cgatgatcgc ctctatggcg agcgagtcgt agaagccgac
     3121 ggaggcggtc ttgttgctga atcctcccgt cgcgacggtg ccgaacatgt gcacgacgga
     3181 gtcatagagc ggcatcccgg cgagtagcag ggctacggtc tcggcgattg aaatgatgag
     3241 gtagatgact acgagggcct tggccgtgtc ggcgattctc ggggtcaggc ggggctgggt
     3301 gagtcccgac atctccgcgc cgagcaggcg ggcggcaccg accccgagca gcggcgctac
     3361 ggcgacgaag aggaccacga tccctattcc tcccagccag tgcgtcatgc tccgccagaa
     3421 cagtatggac ggcgtctgtg cctcgatatc cgagagcagc gtggcccccg tcgtcgtaaa
     3481 cccgctcatc gcctcgaacg acgaatcgag gaggctatgg aacgtgccct cgaccagaaa
     3541 cggggtcccg ccgagtagtg ccgcgagcgt ccaggccagg gtgacggaca ggtacacgtc
     3601 cctgttcgag acgtactcgg gtgagcggcc acgagggcgg gctgcgcgga agcccagtgc
     3661 gcccgcggcc accatcacag cggccggcag caggaagctc caccacgagc cgtcctcgta
     3721 gagcaacgag agcgcgagag ggatgagtag cgacagccct atcgcaagga tcacacccgc
     3781 gttcaccctg atgatgaccc ttgggttcaa aggctatggg gcgaaaaggt tctcgacctc
     3841 ttccacggcg tcctcgatag tgaagacgac ggcgtcgtca ccgggcctga gaacct
//
LOCUS       DATAIR010000497         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_309544, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000497 DATAIR010000000
VERSION     DATAIR010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309544"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VHM69_11925"
     CDS             <1..949
                     /locus_tag="VHM69_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2741147.1"
                     /translation="SVDVAATTGSGPAGGSGFVVDEAGHIVTNQHVVEDAQDISVRFA
                     EGTRRSAEIVGEDPSTDIAVIKVDAPRGALEPLTLGDSDSVGVGEPVIAIGNPLNVGI
                     SVTTGIVSGVGRPIKAPNNYTINDAVQTDAAINPGNSGGPLLDSRGTVIGVNAQIASE
                     SGGFEGVGFAVPIDTVKSVVKQLITEGKVVHGYIGVSMFELGIDELSAYTGLSEESLR
                     EQYGFPGDGAIVSEVTPDGPAEKAGIRGGEEKDIEGIAVPIGDVITEIEGEPISTSEE
                     VIEVVNSLKPGERLPMTVVTPGEDERSVVVKVGTRPEGV"
     gene            953..1654
                     /gene="rpiA"
                     /locus_tag="VHM69_11930"
     CDS             953..1654
                     /gene="rpiA"
                     /locus_tag="VHM69_11930"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX2741148.1"
                     /translation="MSGDELKREAAFAAVERYVESGMVVGLGTGSTASFAVRRIGELV
                     ASGELRDIKGVPTSARTTALANEMGIPLATLAEARPRFTIDGADEIEPGLALIKGRGG
                     ALLREKIVAAASEEGLIVVADSGKLVDTLGAGPLPVEIDSFGWEATLEALASLGCEPE
                     LRMDWTDPGRPFVTDGGHYTADCTFDSIQEPASLEVEIKHIPGALECGLFVSLARAAV
                     VASKNGTESFEEKPR"
     gene            complement(2002..2268)
                     /locus_tag="VHM69_11935"
     CDS             complement(2002..2268)
                     /locus_tag="VHM69_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741149.1"
                     /translation="MERHLIDRVIDEAKGEGANLVIWDRRDTFTVESDDVKEIEINDD
                     HLRVKMQDGKAIVYVEYDSIYKLVVEKERATRTGIRAGFGAASS"
     gene            2438..2971
                     /locus_tag="VHM69_11940"
     CDS             2438..2971
                     /locus_tag="VHM69_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2741150.1"
                     /translation="MKGLARKIRDLDRALFRAVFGVKWTPLTSVMRAFTVVGTAGALW
                     GFLAAVAFLFTGLKPWHLVIPWTAVAASWTVAEGAKYLFNRARPFIWDTEIAPLIKTP
                     SSSSFPSGHSATAAAGAITLSFLYPPFAPAFVVAGFLIVLSRVYLGVHFPVDVLAGVA
                     IGTATSGVVLAVAELLM"
     gene            complement(3019..>4736)
                     /locus_tag="VHM69_11945"
     CDS             complement(3019..>4736)
                     /locus_tag="VHM69_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX2741151.1"
                     /translation="RGDHGAAVTTAGRALDLVSAGDWPAQRVYAHLRLADLLPDEAEA
                     HLLEAQRLAERLALPHLRYRLYERLGRLRRLQYRDEEARGLLEAAVDDIERLRGTVAH
                     ETLRASFLRDKTAAYEELLKLYLARGTREDWRRAFAVAERAKSRALADLLTGVAGESA
                     TIADDALEGRILALQSELNVAYTQMLGGTEDPGIPLPDLRGRATSLEAEISRLQLRAA
                     VAPDPFAPTAPSDSLEDLPSDVALLAYHGVGDEIAAFISTRDGVEAVRNIGSAAALAR
                     LAQQLDVQWDRLSTGREFARRHMALLERSTKQVLASLYEKLVRPLEPHLNEAGATSAP
                     RRLVIVPHGLLHSVPFHALFDGESYLLERFEISYAPSAKVYSLCQTRTPRGLDKVLAL
                     SVPDPSIPAVTDEAGAVARHFPEVEVLDDRRATAAALREKAPGCDALHLACHGMFRSD
                     NPMFSSLKLHDGWLTAADVLHLDLADALVTLSACESARNEVFAGDELIGLTRAFLGAG
                     AATLVASLWLVQDETTPELMETWYERLSAGVGKASALREAQLALKSKYPHPYYWAPFV
                     LIGQR"
BASE COUNT          838 a         1487 c         1527 g          884 t
ORIGIN      
        1 gagcgtcgac gtggcagcca cgaccgggtc cggccctgcc ggcggctccg ggttcgtggt
       61 ggacgaggcg gggcacatag tcaccaacca gcacgtggtc gaggacgcgc aagacatctc
      121 cgtcaggttc gccgaaggca cgcgcaggtc ggccgagatc gtcggggagg acccttctac
      181 ggacatcgcg gtcataaagg tggacgcgcc gcgcggggcg ctggagccgc tcacgctcgg
      241 agactcggac tccgtgggcg tcggggagcc ggtaatcgcc atcggcaacc ctttgaacgt
      301 cgggatcagc gtcaccaccg ggatcgtgag cggcgtcggc cgtcccatca aggccccgaa
      361 caactacacc ataaacgacg ccgtccagac cgacgcggcg ataaaccctg gcaactcggg
      421 cgggccgcta ctggattcgc gcggcacggt cataggggtc aacgcgcaga tagcctccga
      481 gtcaggtggc ttcgagggcg tgggattcgc cgtcccgatc gacaccgtca agagcgtggt
      541 gaagcagctc ataaccgaag ggaaggtggt gcacggctat atcggggtat ccatgttcga
      601 gctgggcata gacgagctct ccgcctacac cggcctctcc gaagaatcgc tcagggagca
      661 gtacggcttc cctggcgacg gtgccatagt cagcgaggtg acgcccgacg gtccggcgga
      721 gaaggccggc atccggggag gggaggagaa ggacatcgag ggaattgccg taccaattgg
      781 agacgtcata accgagatcg aaggcgaacc catctccact tcggaggagg tcatagaggt
      841 cgtcaactcc ctcaagccgg gcgagaggct cccgatgacc gtagtgaccc ctggcgagga
      901 tgaacgcagc gtagtggtga aggtcggtac caggcccgag ggggtttagg agatgtcagg
      961 ggacgagttg aagcgcgagg ccgctttcgc ggcggtggag cgttacgtcg agagcgggat
     1021 ggtggtaggt cttggcaccg gatctacggc cagcttcgcg gtgcgcagga taggggaact
     1081 cgtggcctcg ggggagctga gggacatcaa gggtgtcccg acctctgccc ggaccacggc
     1141 ccttgccaac gaaatgggca taccgctggc gacgctcgcg gaggcccgtc cacgcttcac
     1201 catagacggc gcggacgaga tcgagccggg gctggcgctc atcaagggtc gcggaggggc
     1261 gctccttcgg gagaagatcg tggccgcagc cagcgaggag ggcctgatcg tggttgccga
     1321 ctcgggcaag ctggtggaca cactcggtgc cggtcccctg cctgtcgaga tagactcctt
     1381 cggctgggag gcgaccctcg aagccctcgc ctctttaggc tgcgagccgg agctcaggat
     1441 ggactggacc gatccgggtc gtcccttcgt cacggacggc ggccactaca cagccgactg
     1501 cacgttcgac tccatccaag aacctgcttc actcgaggtc gagatcaaac atatccccgg
     1561 cgccctcgag tgcggcctct tcgtcagcct cgcacgggcc gcagtcgtcg caagcaagaa
     1621 cggcaccgag tccttcgaag agaagcctcg atagcaatca gctatggcgg ttctcgaaaa
     1681 ggatgagcta ctccttcgga gaaggcatca ggcatcaggt ctttttgctg atgcctgaag
     1741 cctgaaacct ttactcacct caggttcact ctttcagcaa accgctctaa tcagcatttc
     1801 agcacgccgc cttgcaggca gcttgtcagc acttcagcat ctcagcaagg gcgtgtggat
     1861 agtaaccccc gttcgagaga gcagctcctt ccctatagct ttcgctgcgt gagcacctcc
     1921 atccgaggaa aagctgaagg ctgaaagcga gggccgcaag gcgccgtgct gacaagcgga
     1981 ggcgaagcct cagcgtgctg cctagctgct tgcggcgccg aagccggcgc ggatgccggt
     2041 gcgggttgcg cgctccttct cgacgacgag cttgtagatg gagtcgtact cgacgtagac
     2101 tatcgccttg ccgtcctgca tcttgacgcg caggtggtcg tcgttgatct ctatctcctt
     2161 cacgtcgtcc gactccacgg tgaaggtatc gcggcggtcc cagatcacga ggttcgcccc
     2221 ttcgcccttc gcctcgtcta tgaccctgtc gatcaaatgc ctttccatct ctctaacccc
     2281 cggttgtgaa atgtgtttgc agaacatccg tctcattcta ctttagaaaa cgggtgtggt
     2341 agggaggagc accactctac cgttgaagaa gaccgtactt atggcaggtc tctagtctac
     2401 aatagccgcc ggatggtcca cacgttacgg gaggatcttg aagggtctgg cgcgcaaaat
     2461 aagggacctc gaccgggcgt tgttcagggc ggtcttcggt gtgaagtgga cccccctgac
     2521 gagcgtgatg cgcgccttca ccgtcgtagg gacggcgggc gctttgtggg gattcctcgc
     2581 tgctgtggcg ttcctgttca ccggcctgaa gccctggcac ctcgtgatcc cctggacggc
     2641 agtagccgct tcctggacgg tggccgaggg cgccaagtac ctgttcaacc gggcccgtcc
     2701 ctttatctgg gacacggaga tcgcgcccct catcaagacg ccctcctcga gctctttccc
     2761 ctcgggccac tccgcgacgg cggcagccgg ggcaattact ctctcctttc tctatccgcc
     2821 tttcgcaccc gcgttcgttg tggcggggtt cctgatagta ctttcacgcg tctacctggg
     2881 cgtccacttc cccgtcgacg tcctagccgg ggttgcgatt ggtacggcga cctcgggcgt
     2941 agtacttgcc gttgcagagc tacttatgtg aaggagtgcg tgctcgcccg ttgctccgag
     3001 gccctagtat acgtagctct atcgctggcc tattagtacg aagggtgccc agtagtaagg
     3061 gtgtgggtat ttactcttga gtgcgagttg ggcctcgcgc agggccgacg cctttccgac
     3121 gcctgcgctc aggcgctcgt accacgtctc cattagctcc ggcgtcgtct cgtcctgcac
     3181 gagccacaag ctcgccacga gcgtcgctgc tcctgctccc aggaacgccc gcgtcaggcc
     3241 gataagctca tcgccggcga agacctcgtt gcgagccgat tcgcaggcgc tcagggtgac
     3301 gagggcgtcc gcgaggtcga ggtgcagcac gtcggccgcc gtcaaccaac cgtcgtgcag
     3361 tttcaacgat gaaaacatcg ggttgtccga cctgaacata ccgtgacagg ccaggtgcaa
     3421 agcgtcgcac cctggcgcct tctcgcgtag cgccgctgcg gtcgccctcc tgtcgtccag
     3481 tacctctacc tcggggaagt gcctggcgac ggccccggcc tcatccgtca cggccggtat
     3541 cgatgggtcg ggcacgctca gcgcgagtac tttatccaat cctcgcggag tacgcgtctg
     3601 gcagagcgag tagaccttcg cgctgggtgc gtacgagatc tcgaaccgct ccagcaggta
     3661 cgactcgcca tcgaacaggg cgtggaaagg cacggaatgg agcagcccgt ggggcactat
     3721 gacgagcctc cgtggcgccg aggtcgcccc tgcttcgttc aggtgtggtt ccagcggtct
     3781 taccaacttc tcatacagcg aggccagcac ctgtttcgtc gaccgctcga gcaacgccat
     3841 gtgccgccgt gcgaactccc tgcccgtgct caagcggtcc cactgcacat cgagttgctg
     3901 cgcaagacgc gcgagcgccg ccgccgaccc gatatttcgc acggcctcga cgccatctcg
     3961 cgtgctgatg aaggcagcga tctcgtcgcc gacaccgtgg taggccagga gcgccacgtc
     4021 tgaggggaga tcttcgagag aatccgaagg ggcggttggc gcgaacgggt caggcgcaac
     4081 agccgcgcgc aactgcagac ggctgatctc tgcttcaagc gaggtcgccc gcccgcgcaa
     4141 gtcaggtagg gggatgccgg gatcctcggt gcccccgagc atctgggtgt aggctacgtt
     4201 cagctccgat tgcagagcca ggattcggcc ttccagcgcg tcgtctgcga tcgttgccga
     4261 ctctccggcc actccggtga gcagatccgc cagggctctg gacttggccc tttcggcgac
     4321 ggcaaaggca cggcgccagt cctctcgggt tccccttgct aggtagagct tcagcagttc
     4381 ctcgtaggcg gcggtcttgt ctcgcagaaa ggaggcgcgt agcgtctcgt gggcgacggt
     4441 gccccgcagg cgttcgatgt cgtctacggc ggcttcgagc aggccgcgtg cctcttcgtc
     4501 gcggtattgc agacgccgca gtcgaccgag ccgctcgtac aatcggtagc gcaggtgggg
     4561 cagtgcaagg cgttcggcaa gcctctgtgc ctccaggagg tgagcctctg cttcgtcggg
     4621 cagcaggtcg gcgagccgca ggtgggcgta gacgcgttgc gccggccaat ctcccgcgga
     4681 gacgagatcc agcgcccgac ccgcggtagt taccgccgct ccatgatccc ctcgcg
//
LOCUS       DATAIR010000498         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_401784, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000498 DATAIR010000000
VERSION     DATAIR010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401784"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHM69_11950"
     CDS             <1..735
                     /locus_tag="VHM69_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX2741152.1"
                     /translation="EGKSQHAFCARMGREYPYDVRVLANVKPDAYWMDAMLHEFGHAV
                     YDRHVNPRLPYLLRIFAHANTTEAIALMMGTLVDDPGWLRSIAGVEKERLDEDHTKLA
                     VHRRADKMTLARFVLVMYHFEQALYADPDDEDSNSVWWDLVERFLFVDRPPERDEPDW
                     AAVIHIAVAPVYYHNYMLGELISAQLRSYLESHITHGPFYENEVAGRYLLESFFGPGA
                     RENWRDTVLRATGEPLNPEYFVKSLD"
     gene            complement(760..>1648)
                     /locus_tag="VHM69_11955"
     CDS             complement(760..>1648)
                     /locus_tag="VHM69_11955"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX2741153.1"
                     /translation="GCLDTGQTGSIEASPEAASVASTQAVSESTSVASGVPESTVGVA
                     PEGEVSGAEEASSGAKFVARTEMSGGSGYDADTVLAVRHGIHGGYERVVIDLGTGEEP
                     AETIPQWTLASPNGDGLMRVTLPSTSATCVSDGRFGEGLLASYHVVRAPEGGMFVDFL
                     ARKAFLYRVLEIGDPARLVVDFKPTGADLKVPLPKEGGNTVLVEPRSQARISDPLTVS
                     GYSRNFEAANTITLTDSDGKVLVRRTVLSNDWGSTWGYFEATFDLPQFAGKGTLQVGT
                     RSARDGSFEGVEIPVRASR"
BASE COUNT          302 a          566 c          493 g          287 t
ORIGIN      
        1 gaaggcaaga gccagcacgc cttctgcgcc cgcatgggac gcgaataccc ctacgacgtc
       61 agggtgctcg ccaacgtgaa gcctgacgcc tactggatgg acgccatgct ccacgagttc
      121 ggccacgcgg tctacgatcg ccacgtcaac ccgcgacttc cttacttgct gcgcatcttc
      181 gcccacgcca acactacgga agccatagcc ctcatgatgg ggaccctggt cgacgatccc
      241 ggctggctcc gctcgatcgc aggggtagag aaagagaggc tcgacgaaga tcatacgaag
      301 ctcgccgtgc accgccgggc cgacaagatg accctcgccc gcttcgtgct tgtcatgtac
      361 cacttcgagc aggccctcta cgcggacccc gacgatgaag attcgaactc ggtgtggtgg
      421 gacctcgtcg agcggttcct tttcgttgac cgtccacccg aaagggacga gcctgactgg
      481 gcagccgtca tccacattgc cgtcgccccc gtctactacc acaactacat gctgggggag
      541 ttgatctcgg cccagctgag gagctacctg gagtcccaca taacacacgg ccccttctac
      601 gagaatgagg tagcgggccg ctacctcctc gaatccttct tcggtccggg cgcccgcgag
      661 aactggcgcg acacggtcct gcgcgccaca ggagagcccc tgaaccctga gtacttcgtg
      721 aagtctctcg actagagcgg cggagtcagg gcctaggacc tatcggcttg cccggacggg
      781 gatctcgact ccctcgaagg aaccgtcgcg ggcgctccgg gtcccgacct gcagagtccc
      841 tttgccagcg aactgcggca ggtcgaaggt ggcttcgaag tatccccagg tggagcccca
      901 gtcgttgctc aaaacggtcc ggcgcaccag aaccttcccg tcagaatctg tcagtgtgat
      961 ggtgttggcg gcctcgaagt tgcgggagta gccactaact gtgaggggat cggagatcct
     1021 ggcctgcgag cgcggctcga cgaggaccgt attgccgccc tccttcggca acggcacctt
     1081 caggtcagcc cccgtcggct tgaagtcgac gacgaggcgg gcagggtctc cgatctcgag
     1141 caccctgtag aggaacgcct tgcgagcgag gaagtccacg aacatgccgc cctcaggggc
     1201 gcgtacgacg tgatagcttg cgagcagacc ttctccgaac ctgccgtccg agacgcacgt
     1261 cgcgctcgtc gaaggcaggg tcacgcgcat gagaccgtcg ccattcgggc tcgccagggt
     1321 ccattgcggt atcgtttctg ccggctcctc accggtcccg aggtcgatga ccacccgctc
     1381 gtatcccccg tgaatcccgt ggcgcaccgc gagtacggtg tcagcatcgt agccggaacc
     1441 gccgctcatc tccgttctcg ctacgaactt ggccccggaa gaagcctcct ccgccccgga
     1501 gacttcaccc tcgggggcta cccccaccgt agattcgggc acgccgctag cgacggacgt
     1561 gctctccgag accgcctgcg tagatgcgac cgacgcggcc tcgggagaag cctctatgga
     1621 ccccgtctga cccgtgtcca ggcagccc
//
LOCUS       DATAIR010000499         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_125229, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000499 DATAIR010000000
VERSION     DATAIR010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125229"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1264
                     /locus_tag="VHM69_11960"
     CDS             149..1264
                     /locus_tag="VHM69_11960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2741154.1"
                     /translation="MRKPHVVILSGIRWHFLWQRHQSLATLFARAGYPTVFVETTGLS
                     NPRLNRTTLRKLLSRVRRSGGRKSPGERNLTIYAPLAAPPTWKAFRWSNARLFLPRVL
                     RDLGKIAGTRPVVVAYPPTRTTLALISGLDPALVLYDCSDDYEHFPGVPKDIASTERE
                     LLLRADLVSCTSPYLLEKASRVRTDAFLSGPAVDYERFAVLQGPHATAEVRTVCFFGH
                     VSRERIDFDALKAIVEAGFTLRIVGGVDGSLMQMPGVDYRGEVSHAELPEALAGVDAF
                     VLPYRINALTRGISPAKTYECLATGKPVVAAHLPAMRDLGEHIYLAESPQDYVEVLRN
                     IQDLETEERMRARTELASENSWEVRFGELEEALWRAL"
     gene            1249..2016
                     /locus_tag="VHM69_11965"
     CDS             1249..2016
                     /locus_tag="VHM69_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX2741155.1"
                     /translation="MASPVDRVDVLGVGIDPMTVDELQAEVKRLAGSGEVGLVLNVNA
                     HCLNLCYKSPALRGFFNSADVVFCDGAGVMLAARLLGRRIPARITYADWAWKLAAFAE
                     AEGLSLFLLGARPGVAEAAARRLEALHPALRISGVHHGYFDHAAGSTENEAVLREIQA
                     ADPDVLLVGFGMPLQEHWLMENRHRIEAGVALTGGAVFDYVSGELRRGPRFLTDNGLE
                     WLARLLVEPRRLWRRYLLGNPLFALRVLGQRFGMGRR"
     gene            2161..>2944
                     /locus_tag="VHM69_11970"
     CDS             2161..>2944
                     /locus_tag="VHM69_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741156.1"
                     /translation="MTLGLVAFIVMAAPDAGAERHAPPAAERTEGEVVGASIAHPANW
                     TVERERYTFDGTYGFTLWRPEPGSSHDHGGIPAVRVALAEELQPSQVDDEVRRTIAYY
                     VDDNLEVGRQTVRAGAEGHEGVAVGPIPGSTPATRVYVPMDGRVYQIDVYSEAPGEEG
                     LDADDRALLRTLRFEQPSRPVASLGLPRANAPESLYPTSGEEPAPASRGWTSSTSADE
                     KSSGTAGYSVSGAGETRIAEGCWRADPGFWVQVQHGRTANSSS"
BASE COUNT          503 a          950 c          980 g          511 t
ORIGIN      
        1 ggacggatgg cgcagccaca cgagccccag aaggctgaag aggagcgtcg agatactgaa
       61 cctcttcggc ctcgaccggc tctacggcga gggagccatg cccgacccct ccggggcctt
      121 tgccctgatg gacggggagt aggcaggcgt gcgcaagccg catgtggtga tcctctccgg
      181 catccggtgg cacttcctct ggcagcgaca ccagtcgctc gccaccctct tcgccagggc
      241 cggctaccct accgtcttcg tggagaccac ggggctctcc aatcctcgct tgaacaggac
      301 gacgttgcga aagctgctct cccgggtccg ccgttccgga ggacggaagt cgccgggaga
      361 aaggaacctc accatttacg ccccgctcgc ggccccgccg acatggaagg cattcaggtg
      421 gtcgaacgcg aggcttttcc tccctcgggt gctccgcgac ctcgggaaga tagccggtac
      481 ccgtcctgtc gtcgtggcct atccgcctac ccgtacgacg ctcgctctga tctccggcct
      541 cgatcccgcc ctggtcctct acgactgctc ggacgactat gagcacttcc ccggcgtgcc
      601 gaaggacatc gcaagtacgg agcgcgagct cctgctgcgc gccgacctcg tctcatgcac
      661 ctcgccctac ctgctggaga aggcgagtcg cgtcaggacc gacgccttcc ttagcggtcc
      721 cgcggtggac tacgagcggt tcgctgtcct gcagggcccg catgcaactg cggaggtccg
      781 caccgtgtgt ttcttcggcc acgtgagccg ggagcgcata gacttcgacg ccctgaaggc
      841 catcgtcgag gcgggcttca cgttgcgaat cgtgggcggc gtggacggga gcctcatgca
      901 gatgccagga gtagattaca ggggagaggt ctcccacgcg gagcttccgg aggcgctcgc
      961 tggcgtcgac gctttcgttc tgccgtacag gatcaacgct ctcaccaggg gcatctcgcc
     1021 ggccaagacc tacgagtgtc tggccacagg caagccggtc gtcgcggctc accttccagc
     1081 gatgcgggac ctcggtgagc acatctacct cgcggagagc ccgcaggact acgtggaggt
     1141 tctgcgcaac atacaggatt tagagacaga ggagaggatg cgggccagga ccgagcttgc
     1201 cagcgagaac tcctgggaag tccgcttcgg ggagctggaa gaggctctgt ggcgagccct
     1261 gtagaccggg tggacgtact cggggtggga atcgacccga tgaccgtaga cgagttgcag
     1321 gccgaggtaa agcgcctggc cgggagcggg gaggttggtc tggtcctgaa cgtgaacgcc
     1381 cactgcctca acctctgcta taaatctccg gcgctgcgcg gattcttcaa cagcgcagac
     1441 gtggtcttct gcgacggggc gggcgtgatg ctcgcggcga ggctccttgg gaggcgcatt
     1501 ccggccagga tcacctacgc ggactgggcg tggaagctcg ccgccttcgc cgaagctgaa
     1561 gggctctcgc tcttcctgct cggcgccagg ccaggcgtgg cggaggcggc ggcccggaga
     1621 ctcgaggcgc tacatcccgc gctccgtatt tcaggagtcc atcacggcta cttcgaccac
     1681 gcagcgggca gtaccgagaa cgaggctgta ctgcgggaga tccaggccgc agaccccgac
     1741 gtcctgctcg tcggcttcgg catgcccctg caggagcact ggctgatgga gaaccgccac
     1801 aggatcgagg ccggcgtcgc cctcacgggc ggggccgtct tcgattacgt ctcaggggaa
     1861 ctgcgccgtg gaccgcgctt cctcactgac aacggcctcg agtggctggc acgcctcctc
     1921 gtcgagccgc gacgcctgtg gcgccgctac ctgctcggca accctctctt cgcgctgcgg
     1981 gtgctcgggc aacgattcgg catgggacgg cggtagtcag gcgattctcc cggcatcgtc
     2041 gccggatctc gaaaaagtag gtcgagactc tttaagtctt cgcgctcctg agacataata
     2101 tccggctggt agcgcttaca gctctggagg gggagaatgc gtctcaggat agtatctttt
     2161 ttgacccttg gattggtcgc attcatagtc atggcagcac cagatgcggg tgctgaaagg
     2221 cacgctccac ctgcggccga gaggacggaa ggggaggtcg tcggggcctc catcgcgcac
     2281 cccgcgaact ggaccgtcga acgcgagcgg tataccttcg acggcaccta cggctttacc
     2341 ctttggagac ccgaacctgg ctcgtcgcac gaccacggtg ggatccccgc cgtgcgtgtc
     2401 gcgctcgcgg aagaactgca accttctcag gtcgatgacg aggtcaggcg gaccatcgca
     2461 tactacgtag acgataacct ggaggtgggc cgccagaccg tcagggctgg tgcggaggga
     2521 cacgagggcg tggctgtcgg accgatccca ggcagcacgc ccgccacgag ggtctacgta
     2581 cccatggacg gtagggtcta ccagatcgac gtttactcag aggcgcctgg agaggagggg
     2641 ctcgacgctg acgacagagc gttgctccgt actttgcgct tcgagcagcc ctcccggccg
     2701 gtcgcctccc tcggcctgcc gcgcgcgaac gctcctgagt ccctgtatcc gacgagcggt
     2761 gaggagcccg cgccagccag tcgcggctgg acgtcttcta cctcagccga cgagaagtcg
     2821 tccggtacag ccgggtactc ggtgtcggga gcgggggaga cgcggatcgc cgagggttgc
     2881 tggcgcgctg accccgggtt ctgggtgcag gtccagcacg gccgaacggc caacagcagc
     2941 tctg
//
LOCUS       DATAIR010000500         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_401838, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000500 DATAIR010000000
VERSION     DATAIR010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401838"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VHM69_11975"
     CDS             complement(<1..585)
                     /locus_tag="VHM69_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2741157.1"
                     /translation="MEENIWRSERVPGEVEIAVGRAGDGPDPVICLHGITAQHRAFNA
                     AARYLGPSRGLVGVDLRGRGDSDKPESGYGLEAHAADVVRVLDHLGIESAVLVGHSMG
                     GFVALKTALAYPERVRALVLLDGGWPRVERSPEEMTEEEKQEAAALEEGLARAFRRLD
                     MTFESPEDYLNFWFPDQGLTMDDLPPDLADYYLYD"
     gene            648..1652
                     /locus_tag="VHM69_11980"
     CDS             648..1652
                     /locus_tag="VHM69_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2741158.1"
                     /translation="MDYFESVLDVVGKTPLIRLGKIERDEGIEATLLAKVEYLNPAGS
                     VKDRPAVKMLEVAEKEGLLKPGGTVVEPTSGNTGAGLALAAAVKGYRCICVMPDKVSK
                     EKQDLLKAMGAEVVITPAAPPEDPESYYAVAERLANEIPGGFKPGQYENPANPLAHYE
                     TTGPELWRQTDGRITHFVVGMGTCGTITGTGRYLKEQNPEVRVVGVDPEGSIFSDPDN
                     IHQYAVEGVGEDFYPGNYDESVVDKVIQVFDRESFLMTRRLMSAEGLFVGGSCGMAVV
                     GAVRAAKDLPEGAVVVVLLPDTGRNYVSKVFDDEWMLANSGATPEDLQRPYRVFEEGS
                     "
     gene            1649..>2531
                     /locus_tag="VHM69_11985"
     CDS             1649..>2531
                     /locus_tag="VHM69_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2741159.1"
                     /translation="MKYRGFATRAIHAGQEPDPATGATIVPIYQTSTYTQEAPGQHKG
                     YEYSRTRNPTRTALEECVAALEGAEYGLAFASGLAATIAVLSLLSRGDHVVAGDDLYG
                     GTYRLFDKVLPRTGGLDFSYADTTDPASVEEALRPETKLLWIETPTNPMLTLSDIAKL
                     SEMAHEHGAVVVVDNTFASPYFQTPLALGADIVVHSTTKYMGGHSDVIGGAVAVSDDG
                     LYEGIKFYQNAAGGVPGPFDSWIVLRGLKPLAVRMRQHEENAIAVAEFLQGHPEVETV
                     LYPGLPAHPQHDLAKRQM"
BASE COUNT          474 a          853 c          797 g          407 t
ORIGIN      
        1 gtcgtagagg tagtagtccg cgagatcggg cggcaggtcg tccatcgtca gcccctgatc
       61 cgggaaccag aaattcaggt agtcctccgg gctctcgaag gtcatgtcga ggcgtctgaa
      121 ggcccgtgcg aggccctcct cgagcgccgc ggcctcctgc ttctcctcct cggtcatctc
      181 ctctggagac ctctcgacgc ggggccagcc gccgtcgagc aggacgaggg cgcgcacgcg
      241 ctcggggtac gcgagcgccg tcttcagggc gacgaagccg cccatggagt gccccaccag
      301 tacggcgctc tcaatcccga ggtggtcgag gacgcggacc acgtcggccg cgtgcgcttc
      361 gagcccgtag ccggattccg gtttatccga gtcgcccctt ccgcgcaggt ccacgccgac
      421 gagaccacgt gagggcccca ggtaccgtgc agcggcgttg aaggccctgt gctgggccgt
      481 tatgccgtgc aggcagatta caggatccgg gccgtctcct gcgcggccta ccgcgatctc
      541 cacctcgcct gggacgcgct cgctcctcca gatgttctct tccataggcg gggattatac
      601 gcgaggcgcg ccgcgttcgg aggggccgct ggtggtaatc taccggcatg gactatttcg
      661 agagtgtgct tgatgtcgta gggaaaacgc ccctgatccg gctcgggaaa atagaacgcg
      721 acgagggcat agaggccacc ctcctcgcca aggtcgagta cctcaacccc gccggctccg
      781 tgaaagacag gcccgccgtg aagatgctcg aggtcgcgga gaaggaaggt ctcctcaagc
      841 ctggcgggac cgtcgtcgag ccgacgagcg ggaacacggg ggccggcctc gcgctggccg
      901 ccgcggtcaa gggctaccgc tgtatctgcg tgatgcccga caaagtgagc aaggagaaac
      961 aggacctgct gaaggctatg ggcgccgagg tcgtcatcac gccggcagcg ccgccggagg
     1021 accccgagag ttactacgcc gtcgccgaga ggctggcgaa cgagatcccc ggcggcttca
     1081 agcctggcca gtacgagaac cctgccaacc ctcttgccca ctacgagacg acgggccccg
     1141 agctctggcg ccagacggat ggccgcatca cccacttcgt cgtaggcatg ggcacctgcg
     1201 gaaccatcac gggcacgggc agatacctca aagaacagaa ccccgaggtc agggtcgtcg
     1261 gggtcgaccc tgagggttcg atcttctccg accccgacaa catccaccag tacgccgtcg
     1321 agggcgtcgg agaagacttc tatcctggaa actacgacga atccgtggtt gataaggtga
     1381 tccaggtatt cgaccgcgaa tccttcctta tgacccgccg cctgatgtct gcagaaggac
     1441 tcttcgtcgg aggctcgtgc gggatggccg ttgtcggcgc ggtcagggcc gcgaaggact
     1501 tgcccgaagg cgccgtggtc gtcgtgctcc tgcccgatac agggcgcaac tacgtctcca
     1561 aggtcttcga cgacgagtgg atgctcgcca actcgggcgc cacccctgag gatctccaga
     1621 ggccctaccg cgtcttcgag gagggatcat gaagtatcga ggcttcgcca cgcgcgccat
     1681 acacgcgggg caggaacccg accctgctac aggggcgacg atcgtcccga tctaccagac
     1741 ctccacctac acccaggagg cgccgggcca gcacaaaggc tacgagtaca gcaggacacg
     1801 caaccctacc cgcacggccc tcgaagagtg tgtcgccgcc cttgaaggcg ctgagtacgg
     1861 cctcgccttc gcctcagggc tcgcggcgac gatcgccgta ctgagcctcc tctcgcgtgg
     1921 ggaccacgtc gtcgcgggag acgacctcta cggcgggacg tacaggctct tcgacaaagt
     1981 cctcccccgc acaggcggcc tggacttcag ctacgcggat accacggacc ccgcctccgt
     2041 cgaggaggcg ctcaggcccg agacgaagct gctctggatc gagacgccga ccaacccgat
     2101 gctcaccctc tcggacatag cgaagctctc cgagatggca cacgaacatg gagccgtagt
     2161 ggtggtggac aacaccttcg cctctcctta ctttcagacc ccactcgcac ttggcgctga
     2221 catcgtcgtc cactcgacga ccaagtacat gggcggacac tcagacgtca taggcggggc
     2281 cgtcgcggta tcagacgatg ggctctacga ggggatcaag ttctaccaga acgccgccgg
     2341 cggcgtaccc ggtcccttcg actcctggat cgtgcttcgc gggctgaagc ccctcgccgt
     2401 ccgtatgcgc cagcacgagg agaacgccat cgccgtcgcc gagttcttgc aaggccaccc
     2461 cgaggtggag acggtgttat accctggcct cccagcacac ccccagcacg atctggcgaa
     2521 gaggcagatg a
//
LOCUS       DATAIR010000501         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_125298, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000501 DATAIR010000000
VERSION     DATAIR010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125298"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VHM69_11990"
     CDS             <1..227
                     /locus_tag="VHM69_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2741160.1"
                     /translation="GVIGHVQIADSPGRGEPGTGEIHYPYVLAALEDLDYGGYVGLEY
                     NPTTETTEESFGWLPEELSGGDVAVSDLKL"
     gene            241..1131
                     /locus_tag="VHM69_11995"
     CDS             241..1131
                     /locus_tag="VHM69_11995"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX2741161.1"
                     /translation="MAEKVGFIGLGIMGKPMAKNLIDADYDLVVYNRTREKAEELASE
                     GATVVGSPKEVAEQSDVIITMLPDSPQVEEVLTGEDGVLEGVSEGALIVDMSTISPVV
                     TEELSEKAGEKGASMLDAPVSGGDVGAIEGTLSIMVGGSEDDFERAKPLFDVMGKTVT
                     HVGPSGTGQVVKAANQIVVALTIEAVSEALVLGSKGGVAPEKILDVLGGGLAGNKVME
                     LKREKMLTHTFDPGFKIELHHKDLGIALAAGREYGVTLPVTAIVDQMLETLKMRGKGD
                     RDHSAILTLIEESSGHEIGS"
     gene            1162..2616
                     /locus_tag="VHM69_12000"
     CDS             1162..2616
                     /locus_tag="VHM69_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2741162.1"
                     /translation="MDRGRLIETMQGILGPQGVIYEREQLRTYECDGLMNYRVIPDLV
                     VLPESAEQVQQVVKTCYDEGIPFVARGSGTGLSGGALPVESGVLIVLSRMRKIIEVDL
                     ENQRIVVEPGVINIWVSQEVADDGYYYAPDPASQIVSSIGGNLAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVVHIGGGKAPDAPGYELLGAFVGSEGTLGIATKITLRLVRKPE
                     SVRTVLAAFKDTEEAGGAVSGIIGAGIVPAAIEMMDALTIEAVETQVAPGFPEAGAIL
                     IVELDGPEAEVASQFDHVLDVCQMNEASEIRVAEDDEERALFWKGRKAAFGAMGSIAN
                     DYYVQDGVVPRTELGAVLKKISNLSDYYGLRVANVFHAGDGNLHPLVLYDNDAPGEAE
                     KAESLAGDIVFVCLEHGGSLTGEHGIGEDKKKYMPKMFTADDMNVMQLLRCAFDPHQI
                     CNPGKIFPTPRLCGERPGPFHMHPVQKAGLAENF"
     gene            2616..3866
                     /locus_tag="VHM69_12005"
     CDS             2616..3866
                     /locus_tag="VHM69_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2741163.1"
                     /translation="MQTKRAPLEDLQGIVGKDNVREATAGDVVEGVESSLVVEPGSVE
                     EISAVMKLASREGLAVSPRGGGTKLDLGAPPRELDLILSTARMNEIIEHVPGDQIVRT
                     QSGLKLAALQEKLAGANQMLAIDPPEKDATVGGVVAANSSGPRRYKYGTVRDLIIGIT
                     VVLADGTVAKAGGKVVKNVAGYDLSKLFTGSFGTLGVIADANFRLHPRPESARTVAVE
                     VETTQAARDAAQAIVHSQVEATAVELHWSDEAKLVTVLLESIPGGIDAKEEAASFLLK
                     GFGEVRTVEDEDSLGHDITGDEVEIKIGAPPAELASVLDSLLDAAERKDVTARITGHA
                     GTGVTYAFLSGEEDAIVEVVEEVREIRTRSGGSVVVRRAPASFKNRVDVWGPAGDYLG
                     LTRRVKEKFDPGYFMNPGRFIGGI"
     gene            3866..>4581
                     /locus_tag="VHM69_12010"
     CDS             3866..>4581
                     /locus_tag="VHM69_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2741164.1"
                     /translation="MTERNGGRNGSEDRYGATDATERRSPEGTAREQAMAGAIGAGSP
                     EDVGQDIGEASSLADEATVGAGRRETGTVSFPAFDAHHPPDKDLIDDCVHCGFCLPTC
                     PTYVLFGEEMDSPRGRIYLMNKGLTEEPLNDQMVRHFDLCLGCMACVTACPSGVQYDK
                     LIEATRGQVERRYERAPEDKAFREMIFQFFPYPNRLRAIAAPMRLYQRFGIGDKLRKA
                     GVMQRIPAKMRAMEALLPDLP"
BASE COUNT          922 a         1384 c         1549 g          726 t
ORIGIN      
        1 tcggggtgat cgggcacgta cagatcgccg actcccctgg tcgcggtgag cctggaacag
       61 gggagatcca ctacccctac gtcctcgcgg ccctcgaaga cctcgactac ggcggctacg
      121 tcggcctgga atacaacccg acgacggaga cgaccgagga gagcttcggc tggttgcccg
      181 aggaactcag tggcggggac gtggccgtat ccgacctgaa gctatagaga ggggagcgcg
      241 atggctgaga aggtgggttt tatagggctt gggatcatgg gcaaaccgat ggcgaagaac
      301 ctcatcgatg cggactacga tctcgtcgtg tacaaccgca cccgcgagaa agccgaggag
      361 cttgcctcag aaggcgcgac ggtcgttggc agcccgaaag aagtcgccga gcaatcagac
      421 gtcatcatca ctatgctccc ggattcgccg caggtcgagg aggtgctgac cggggaggat
      481 ggtgtactcg aaggagtcag tgaaggcgcc ctgatcgttg acatgagcac gatctctccc
      541 gtggtcactg aagagctatc tgagaaggcc ggagagaagg gtgcttcgat gctcgatgcg
      601 cctgtcagtg ggggagacgt cggggccatt gaggggacgc tctcgatcat ggtcggaggg
      661 agcgaggacg acttcgagcg ggcgaagccc ctgttcgatg tcatgggcaa gaccgtgacc
      721 cacgtcgggc cttccgggac cggccaggtc gtcaaggctg ccaaccagat cgtcgtcgcg
      781 ctcaccatcg aggctgtctc cgaggcgctg gtgctcggct ccaagggagg ggtcgcgccg
      841 gagaagatcc tggacgtact gggcggggga ctcgcgggca acaaggtcat ggagctcaag
      901 cgggagaaga tgctcactca taccttcgat ccgggcttta agatagaatt gcaccacaag
      961 gacctgggga tagcgctcgc ggcgggcagg gagtacgggg taacccttcc ggtcaccgcg
     1021 atcgtggatc agatgcttga aacattgaag atgaggggaa aaggggatcg ggaccactcc
     1081 gcgatcctga cgctgataga agaatcgtcg ggacacgaga tagggagcta gagaagggct
     1141 ccgaaagagg gaggacttta catggatcga ggacgactca tagagacgat gcaggggatc
     1201 ctcggcccgc agggtgtgat ctacgagcgc gagcagctac gcacctacga gtgcgacggc
     1261 ctcatgaact accgcgtgat ccccgacctc gtcgtcctcc ctgagtccgc ggagcaggtc
     1321 caacaggtag tgaagacctg ctacgacgag ggcatcccct tcgtcgcgcg gggctcggga
     1381 accgggctgt ccggtggagc tctccctgta gagtcaggag tcctcatcgt cctgagcagg
     1441 atgcgcaaga tcatcgaggt ggatttagag aaccagcgca tcgtcgtaga gcctggcgtc
     1501 ataaacatct gggtctccca ggaggtcgct gacgacggct actactacgc ccccgaccct
     1561 gcaagccaga tcgtctcctc catcggcggc aacctcgctg agaactcggg tggcgtccac
     1621 tgcctcaaat acggcttcac cacgaaccac gtgatgggcg ccgaggtcgt gctgcccgac
     1681 ggctcggtcg tccacatcgg tggcggcaag gcccccgacg ccccaggcta cgagttgctc
     1741 ggggctttcg tcggctccga aggcaccctt gggatagcga cgaagataac gctcaggctc
     1801 gtgcgcaagc ccgagtcggt gcggacggtg ctcgcggcgt tcaaggacac ggaggaggcg
     1861 gggggtgccg tatccgggat catcggcgcc gggatcgtac cggcagcgat agagatgatg
     1921 gacgccctca ccatcgaggc ggtcgagacc caggtcgcgc cgggctttcc cgaggccgga
     1981 gcgatcctga tcgtcgagct cgacggtccc gaggccgagg tcgcgagcca gttcgaccac
     2041 gtcctcgatg tctgccagat gaatgaggcc tcggagataa gggtcgcgga ggacgacgag
     2101 gagcgcgccc tgttctggaa gggacgcaag gctgccttcg gggcgatggg ctccatcgcg
     2161 aacgactact acgtgcagga tggcgtcgtg ccgcgcacgg agctcggggc cgtcctcaag
     2221 aagatctcca acctcagcga ctactacgga ttgcgtgtcg ccaacgtttt ccacgcgggg
     2281 gacggcaatt tgcaccctct ggttctctac gacaacgacg cgcctggcga ggccgagaag
     2341 gcggagtcgc tcgccgggga catcgtgttc gtctgcctcg agcacggcgg ctctctcacg
     2401 ggagagcacg gcatagggga ggataagaag aagtacatgc ccaagatgtt caccgccgac
     2461 gacatgaacg tcatgcagct cctcaggtgc gcgttcgacc cgcaccagat ctgcaatccg
     2521 ggcaagatct tccctacccc tcgcctctgc ggggagcgcc caggaccctt ccacatgcac
     2581 cctgtccaga aggccggact cgcggagaat ttctaatgca gacgaagaga gcgcccctgg
     2641 aggacctgca aggcatagtc ggtaaagaca acgtgcgcga ggcgaccgcc ggggacgtgg
     2701 tcgaaggtgt agagtcttcc ctcgtcgtgg aaccaggctc ggtcgaggag atcagcgcgg
     2761 tcatgaagct ggcgagccgc gaaggactcg ccgtctcccc gcgcggcggc gggacgaagc
     2821 tggatctggg cgctcctcca cgcgaactcg acctcatcct gagtaccgcg cgcatgaacg
     2881 agatcatcga gcacgtgccc ggagaccaga tcgtgcgtac ccagtcaggg ttgaagctcg
     2941 cagccctgca ggagaagctc gccggggcga accagatgct cgccatcgac ccgccagaga
     3001 aggacgccac ggtcggaggc gtcgtcgcgg ccaactcatc aggcccgagg cgctacaagt
     3061 atggaacagt gcgcgacctc atcatcggga taacggttgt tctggccgac gggacagtgg
     3121 cgaaggccgg cggcaaggtc gtcaagaacg tcgccggcta cgacctctcg aagctgttta
     3181 caggttcctt cgggactctc ggggtgatcg ccgacgccaa cttcaggcta cacccgcgcc
     3241 ccgaatcggc gaggacggtc gccgtcgagg tcgagaccac gcaggccgcc agggacgccg
     3301 cccaggccat cgtccactct caagtcgagg cgacggccgt cgagctgcac tggagcgatg
     3361 aagcgaagct ggtaacggtg ttgctcgaaa gcatcccagg aggtatcgat gcgaaggaag
     3421 aagcggcctc gttcctgctg aagggattcg gcgaggtccg tacggtcgag gacgaagata
     3481 gtctggggca tgacatcacc ggcgatgagg tggagatcaa gatcggggcg ccgcctgcgg
     3541 agctcgcaag cgtcctcgac tcgctcctcg acgccgcaga acgcaaggac gttacggccc
     3601 gcattactgg ccacgctggg acgggcgtca cctacgcatt tctgtccggc gaggaggacg
     3661 cgatcgtcga ggtcgtcgag gaggtgcgtg agatccgtac ccgcagcggc ggtagcgtcg
     3721 tggtacgccg cgctccggct tcgttcaaga acagggttga cgtgtggggg ccggcgggag
     3781 actatctcgg tctcacgcgg cgggtgaagg agaagttcga tccggggtac ttcatgaacc
     3841 ccggaaggtt cataggaggc atctagtgac cgaacgcaat ggcggtcgaa acgggtcgga
     3901 ggaccgctac ggcgcgaccg acgccaccga gcggcgaagc cccgaaggca cggcccgcga
     3961 gcaggcgatg gctggtgcga tcggcgcagg cagccccgag gacgtgggcc aggatatcgg
     4021 cgaggcgagc tctctggccg acgaggcaac cgtcggagcg ggacgccgcg agacgggtac
     4081 ggtcagcttc cccgccttcg atgcccacca cccgccggat aaggacctca tagacgactg
     4141 cgtccactgc ggcttctgcc tcccgacctg cccgacctac gtcctcttcg gcgaggagat
     4201 ggacagcccg cgcgggcgca tctacctcat gaacaagggc cttaccgagg agccgctgaa
     4261 cgaccaaatg gtgcgccact tcgacctctg cctcggctgc atggcctgcg ttaccgcgtg
     4321 cccttccggc gtgcagtacg acaagctcat agaagccacg cgcgggcagg tcgagcggcg
     4381 ctacgagcgg gcgcccgagg acaaggcatt cagggagatg atcttccagt tcttccccta
     4441 tccgaaccgc ctgcgggcaa tcgcagcccc gatgcgcctc taccagcgct tcgggatcgg
     4501 cgacaaactc cgcaaagcgg gcgtcatgca acgcatcccg gccaagatgc gcgcgatgga
     4561 ggcgctcctg cccgacctgc c
//
LOCUS       DATAIR010000502         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_447675, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000502 DATAIR010000000
VERSION     DATAIR010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447675"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1791)
                     /locus_tag="VHM69_12015"
     CDS             complement(121..1791)
                     /locus_tag="VHM69_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2741165.1"
                     /translation="MTNARMTPATRPGNLRRFSQISRVLVRHGFGFVFDVRRDRREKK
                     GLQEFLAPNFGVRLRRTLDDLGPTFVKFGQVLSTRSDILPESVLSELQKLQDTATPMP
                     AGVAQEIIEEELGAPIEEVFASFDPVPLGSASIGQVHRAVLRDGDTVAIKVQRPEAPG
                     RVEGDLELMRDFAALLDRRFGRRLFVDVRGLVAEFEVVIRRELDYSTEAENARRFAAN
                     FAETPVIIPGVYLELSTPRVLTEEYIEGTRFRDIRPLTLTPSERRRVASMGAEAIFKM
                     AFEDGFFHGDPHPSNLLLTPDGELALLDFGMVGYMSRGDIEALSRLFIAVIQRDASAA
                     LRGLERLGVRYATEVRADLVRDLREFLNKYSALSVGEVTLGQALSELIALARRYRLKM
                     PPVFPLLTKALVTAEGLARSIDPTLNVYEVARPYAQRLLTERYKPGAVLEVVEERALE
                     YSRYVEDYPEQIRLLLTELADGELEVQLKHGGLDELIGSVDVLANRLVFAVVTAALLV
                     GSAMLGAFELGGPKVPYIGVHIISVAGFTLALILATILLTIIFRSERL"
     gene            complement(1807..2187)
                     /locus_tag="VHM69_12020"
     CDS             complement(1807..2187)
                     /locus_tag="VHM69_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX2741166.1"
                     /translation="MRGTVGERLERLILLQIGAAAATRDKVQEVVDSLVEQGRVEREE
                     GRTVVDDVMRRARERSSGARSLVDASVQQGLRRGGLPTREDHEDLVFRIEQLEHRVRM
                     LEDHPAAPPPQAGEPEGPETPPGL"
     gene            complement(2303..3193)
                     /gene="secF"
                     /locus_tag="VHM69_12025"
     CDS             complement(2303..3193)
                     /gene="secF"
                     /locus_tag="VHM69_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX2741167.1"
                     /translation="MFEKVDFVGGWKLYFSISAILLLAGVIAIALGGLNLGIDFQGGA
                     QFTVASAERQLSDAEIRAALPDDLAGDAVITTLGQNGYEIRTPVVSGQEEQQQIRDSL
                     SQAFGGEVSVTTVSPAFGEQLRNQALQAVVAALLIIVAFISIRFEFAFALAAMAALVH
                     DILMTVGFYAIVGREVSLVTVVAVLTVLGYSIYDTIIIFDRIRENAPTVGYNRRRFDE
                     MINRSIRQVVRRSIYTSIFTLIPITALLIFGEATLSDFAFALLVGIVAGTYSSIFIAS
                     PVLSLYKAWRAGKLQLARSN"
     gene            complement(3196..>4147)
                     /gene="secD"
                     /locus_tag="VHM69_12030"
     CDS             complement(3196..>4147)
                     /gene="secD"
                     /locus_tag="VHM69_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX2741168.1"
                     /translation="YVVHENPDLTGDALQQNGANVEFDQTTGKRIVSLQLTAEGGRQM
                     TELTQKMLSDAAVSGEPAQLAIALDQDIQSAPTVQEPLAQNISISNDSLPDGLPEDEA
                     KQLETVLNTGALPVNMKVISQTTVGPTLGFSSLKSGLLASLVGFGLVLLLLLVIYRAL
                     GVVADLALLIYAFLLWGLIVIIPITVTLPGIAGIVLSIGVAADANVVIFERIKEEIRR
                     GKTPRSAIQAGYDKGFRAILDGNVTTLITAFILFALSSGSVRGFAVLLAIGVVLSMFT
                     AIVITRALLGILADRGIHISPALMGVSKTSIATTQQAKVR"
BASE COUNT          802 a         1315 c         1279 g          751 t
ORIGIN      
        1 gaccaaatta taacagtcag gtcccaggtc tcaggttaca ggcatcaggt cctagacgcg
       61 gaaccgcagt cgcaatagag attctctccc cacgtattcc tgaagcctac aacctgaagc
      121 ctaaagcctt tcagatctga agatgatcgt aagcaggatg gtggccagta tcagggcgag
      181 cgtgaacccg gctacgctga tgatgtgtac gccgatgtag ggtaccttgg gaccgccgag
      241 ttcgaaagcg ccgagcatag ctgacccgac gagcagggcg gccgtcacca cggcgaagac
      301 gagtcggttc gcgagcacgt ccacgctgcc gatcaactcg tccaggccgc cgtgtttgag
      361 ctgcacctct agctcaccgt ccgcaagctc ggtgaggaga agcctgatct gctcgggata
      421 gtcctcgacg tagcgggagt actccagcgc gcgttcctcg acgacctcga gcactgcccc
      481 cggtttatag cgctcggtga ggagcctctg ggcgtatggc cttgcgacct cgtagacgtt
      541 gagcgtcggg tcgatggagc gggcgagccc ctctgcggtc acgagtgcct tggtgaggag
      601 cgggaagacg ggaggcatct tgaggcggta acggcgcgcg agcgcgatga gctcggagag
      661 cgcctgtccg agcgtgacct cgccaacgga gagcgcagag tacttgttga ggaactccct
      721 gaggtctctc accaggtccg cgcgcacctc ggtcgcgtag cgtacaccga gccgttcgag
      781 accgcgcagg gccgccgaag cgtcgcgctg tatcacggcg ataaagagcc tcgataacgc
      841 ctcgatatca ccccggctca tgtatccgac catgccgaaa tcgagcaggg cgagttctcc
      901 atcaggcgtc aggagcaggt tgctcgggtg ggggtctccg tggaagaagc cgtcctcgaa
      961 ggccatcttg aagatcgcct ccgcccccat cgaggcgacg cgacgccgct ctgagggggt
     1021 gagtgtgagt ggacggatgt ccctgaacct cgtcccctcg atgtactcct ccgtcagcac
     1081 cctcggggtc gagagctcca gatagacccc agggatgatg accggcgtct cggcaaagtt
     1141 ggcggcgaag cggcgagcat tctccgcctc tgtggagtag tcgagctcgc gcctgatgac
     1201 gacctcgaac tccgctacga ggccacgcac gtccacgaag agcctgcgtc cgaacctcct
     1261 gtcgagcagc gccgcgaagt cccgcatgag ctcgaggtct ccctcgaccc tgccaggcgc
     1321 ctcgggccgc tgcaccttga ttgccacggt gtccccgtcg cgcagcacgg ccctgtggac
     1381 ctgcccgatg ctcgctgaac cgagcggcac cggatcgaag gaggcgaaaa cctcctcgat
     1441 cggggccccc agttcctctt cgattatctc ctgcgcgacc cccgccggca ttggtgtcgc
     1501 cgtgtcctgg agcttctgca gctcggagag gacgctttca ggcagaatgt cggagcgcgt
     1561 cgagaggacc tgtccgaact tgacgaaagt aggcccaagg tcgtcgaggg tgcggcgcaa
     1621 gcggacgccg aagttcggcg cgaggaactc ctggaggccc ttcttctccc tccggtcacg
     1681 cctgacgtcg aagacgaagc cgaaaccgtg gcggaccagt acgcggctga tctgggagaa
     1741 gcgccggagg ttccccggcc tggtcgcagg ggtcatcctt gcattagtca cgccgggagc
     1801 cgtcggctaa agtcccggcg gcgtctctgg accctccggt tccccggcct gcgggggtgg
     1861 cgccgcggga tggtcctcga gcatcctcac cctgtgctcc agttgctcga tgcgaaagac
     1921 gaggtcctca tggtcctccc tggtcggaag gccgcccctc ctgaggccct gctgaacaga
     1981 agcgtccacc agagaccttg ctccggatga gcgctcgcgg gcgcgtctca tcacatcgtc
     2041 gaccaccgtg cgaccctcct cgcgttccac gcgtccctgt tcaacgaggg agtcgacgac
     2101 ctcctgcacc ttatcccgcg tcgccgcagc agcccctatc tgcaaaagga tcaggcgctc
     2161 cagcctctcg ccaaccgttc cgcgcataat tacctcccga ttatccagtg caccacttat
     2221 atgataacga aggcgcaacc cgcgtgagta ctcaaacatc agtggcttgc gtcaaacgcc
     2281 tgtaccagcg cctcgaagaa aactagttcg agcgcgcgag ctgcaacttc cctgctcgcc
     2341 aggctttgta cagcgacaga acaggtgagg cgataaagat cgagctgtac gtccccgcga
     2401 cgatgcctac gaggagcgcg aaggcgaagt cgctcagggt cgcttccccg aagatcagca
     2461 gtgctgtaat cgggatgagc gtgaagatcg atgtatagat cgaacgccgc acgacctggc
     2521 ggatggagcg gttgatcatc tcgtcgaagc gacgccggtt gtagccgacc gtcggagcgt
     2581 tctccctgat cctgtcgaag atgataatgg tgtcgtagat cgagtaccca agaaccgtga
     2641 ggacggcgac gacggtcacc aggctaacct ccctgccgac gatggcgtag aagcctacag
     2701 tcatcagtat gtcgtgcacg agcgcggcca tcgccgcgag cgcgaaggcg aactcgaagc
     2761 ggatgctgat gaaggcgacg atgatcaaga gcgccgcaac tactgcctgg agcgcctggt
     2821 ttctgagctg ctccccgaac gccgggctga cggtcgtcac gctgacctcg cccccgaacg
     2881 cctggctcag ggagtcgcgt atctgctgct gctcctcctg accgctcacg acgggcgtgc
     2941 gtatctcgta gccgttttgc cccagggtgg tgatgacggc gtcacccgcc agatcgtctg
     3001 gtagcgccgc gcggatctcg gcgtcgctca attggcgctc ggcgctggcg accgtgaact
     3061 gcgcaccgcc ctggaagtcg atcccgaggt tgaggccccc gagcgctatg gcgatgacgc
     3121 ctgcgaggag caggatggcc gatatcgaga aatacagctt ccagccgccg acgaaatcta
     3181 ccttctcgaa cacgcctacc tcaccttcgc ctgttgcgtc gtcgcgatac tcgtcttcga
     3241 gacacccatc aacgccggcg aaatgtggat gccacggtca gcgagaatac cgaggagggc
     3301 gcgtgtgatc acgatggccg tgaacatcga gagcacgacc ccgatagcca acaacacagc
     3361 gaaacctctt accgatccgc ttgatagcgc gaacagaatg aacgccgtga tcagcgtcgt
     3421 cacgttcccg tccaggatag cccggaagcc cttatcgtag ccggcctgga tcgcgctgcg
     3481 tggcgtcttg ccgcgcctga tctcctcttt gatacgctcg aagatgacga cgtttgcgtc
     3541 ggccgcaacc ccgatggaga gcacgattcc agcgatacca ggcaacgtca ccgtgatcgg
     3601 gatgataacg ataagacccc agagtaagaa cgcgtagatg agcagcgcaa ggtcagcaac
     3661 aacgccgagc gccctgtaga tgacgagcag caacagcagc acgagcccga atcccacgag
     3721 cgaagccaga agcccgctct tgagagaact gaagccaagc gtgggtccta cggtcgtctg
     3781 ggagatgacc ttcatgttga ctggcagcgc gcccgtattc agcacggtct ctagctgctt
     3841 cgcttcgtct tcagggaggc catcgggaag actgtcgttg gagatggaga tgttctgcgc
     3901 cagcggttct tgcacggtag gcgcgctctg gatgtcctgg tcgagcgcga tggcgagttg
     3961 agcgggctcg ccgctcacgg cggcgtcgct caacatcttc tgggtgagtt ccgtcatctg
     4021 ccgcccacct tcggcggtca gctggaggct tacgatgcgc ttccccgtgg tctggtcgaa
     4081 ctcgacgttc gccccgttct gctgcagcgc atcgcccgtc aggtcgggat tctcgtgcac
     4141 cacgtat
//
LOCUS       DATAIR010000503         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_245883, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000503 DATAIR010000000
VERSION     DATAIR010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245883"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..635
                     /locus_tag="VHM69_12035"
     CDS             177..635
                     /locus_tag="VHM69_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741169.1"
                     /translation="MDWPTFWSALSAVGQVTAAILAAAALLVTLRTFSKSLTISHYAE
                     LDRMYGELLKLTIDKPHLRDPDAKRTDEQQRQYEYYAHLSWCVVEAIHDRAEDAHLLS
                     IWRTAIEIENNLHREWFDRPENAYMFRPAFREYIAKNFPKPAPAPVAAAS"
     gene            652..1191
                     /locus_tag="VHM69_12040"
     CDS             652..1191
                     /locus_tag="VHM69_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741170.1"
                     /translation="MVAAVRQLTDRDFDTVDRIQRSAYSPDFLEDLAVFADKFARYPD
                     GCWVAEVDGQVVGYLFSHPGHFSAPPGLNKLLEDQAGPPDCYFIHDVAVHPAQRGIGV
                     AQLLVAAAFAHAAARGFPGIALVAVQNSRGFWERRGFRLVSEAMPAVGAVRRSYGSQA
                     HYMIRGSAEPGAAPDPARM"
     gene            1303..2478
                     /locus_tag="VHM69_12045"
     CDS             1303..2478
                     /locus_tag="VHM69_12045"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741171.1"
                     /translation="MATRDEGVRRVYKDHNLQVLWGVTLMAVLGTSSVTPAFPTIVRE
                     LGVSSGQVGLLITVFTLPGIVLTPVFGILSDRYGRKKILVPALLLFGLAGGACAFARS
                     FDLLLTLRFLQGMGAAALGTLNVTVIGDIYDGRERSAALGYNSSVLSVGTASYPAIGG
                     LLATFGWFYPFALPFVAIPIGIIVLFSLRNPEPRNDDRLKDYFGSVWENLRDREVFGL
                     IGASLLTFIVLFGPQISYLPILMNARFDASSFVIGAVLSGASLTTALTSTQLGRLTLR
                     FSERSLLKSAFVLYAIALVAVAFTPSLPMLLVPAVLFGVAQGINLPNVFSLLNAHAPT
                     ENRGAFMAANGMSLRTGQTIGPLFMAYAAGTIGLTGAYLSAAGLAILAFFLVLTLVR"
     gene            2674..3918
                     /locus_tag="VHM69_12050"
     CDS             2674..3918
                     /locus_tag="VHM69_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2741172.1"
                     /translation="MRRGIYARHRLDLSVVDILFGVLSCGRLWERERLEAEVLGLCSL
                     EVEGLVCFSVRSGWDLWLGALGLRAGDEVLVTAVTHPDMVRIIHGHGLRAVPVDIDPE
                     TLAPRPEMLEAALTPRTRVVLVAHLFGGRMDLGPVARVAREHGLLLVEDCAQAFRGPE
                     SLGEAESDLSMYSFGTLKTATALGGAVLRVRDPEVLRKMREFQAAYPVQRRSEYLKKL
                     LKVLVLAVVSRPRPYGLFVLACTRLGHDLDALVNGVVRAFPPGEPIEKLFRRLRRRPS
                     TPLLAMLSRRLGTFDEVRLARRASAGERAARRLRVAGVHPGRNSLQPTHWLFPVVAPD
                     PGALISGLRRRGFDASRVTSNIAVVEAPVGCSSAAEASRMMSGIVFLPVCQDLPSQAS
                     DAMVDLVNECTVAEPVEDLARP"
BASE COUNT          641 a         1296 c         1306 g          833 t
ORIGIN      
        1 cccgcatgag cagccttgcg cagttccgca taagacccgc tgaacaggga ttatatggag
       61 tgtttctgca taataacaag ttcggcggcg gagagcggac ggcgacaatc cgccctgtcg
      121 gttggcgaat accctacagc gtcaatgcac gagggtatct cgcaagaggg gtttccgtgg
      181 actggccaac tttctggagt gcgctgagtg cggtcgggca agttaccgcg gccatcctgg
      241 ctgcggcggc cttgctcgtg accctgcgga cgttctccaa gtcgctcacc atcagccact
      301 acgccgaact tgaccggatg tatggcgagc tactgaagct gaccatcgac aagccgcacc
      361 tccgcgaccc ggatgctaag cggacggacg agcagcagcg gcagtacgag tattacgccc
      421 acctctcatg gtgcgtcgtc gaggccatcc acgaccgggc cgaggacgcc cacctgctct
      481 cgatctggcg aaccgccatc gagatcgaga acaacttgca ccgcgagtgg ttcgaccggc
      541 ccgagaacgc ctacatgttc cgcccggcgt tccgggagta cattgccaag aacttcccca
      601 agccagctcc cgccccggtc gcggcggcat catagccgtg gagacggatt cgtggtagcg
      661 gctgtgcgtc aactgaccga ccgcgacttc gatacggtgg accgcatcca gcgctcggcg
      721 tacagccctg acttcctcga agaccttgct gtgttcgccg acaagttcgc ccgatacccg
      781 gacggatgtt gggtcgccga ggtcgatggg caggttgtcg ggtatttgtt ctcgcacccc
      841 gggcacttct cggccccccc cggcctgaac aagctgctgg aagatcaggc gggtccgccg
      901 gactgctact tcatccacga tgtcgcggtg catcccgcgc agcgcggcat cggcgtggca
      961 cagctccttg tcgcggcggc gttcgcccat gcggcggcgc gtggctttcc tggcatcgcg
     1021 ttggtagccg tccagaactc gcgtgggttt tgggagcgta gggggttccg cctcgtatcg
     1081 gaggccatgc cggccgtggg ggcagttcgc cggtcgtatg ggtcgcaggc ccactacatg
     1141 attcgcggta gcgccgaacc aggcgctgca cctgacccgg cccgcatgta ggcttctcac
     1201 gggacttcat agctcaccga gcgggccggg cagggtagca ggtctgccgg cgtgtgtctc
     1261 cgatcccacc gctgaacgcg ccacctgata gattcattct tcgtggctac gagagacgag
     1321 ggcgttcgcc gggtatataa ggaccataac cttcaggtcc tgtggggcgt gacgctcatg
     1381 gcggtcctcg ggacgtcgag cgtcacgccg gccttcccga ccatcgtacg ggagctcggc
     1441 gtctccagcg ggcaggtggg gctcctgatc acggtcttca ccctccccgg aatcgtgttg
     1501 acacccgtct tcggcatcct ctccgaccgc tacgggcgga agaagatcct ggtcccagcc
     1561 ctcctcctct tcggactcgc gggcggcgcc tgcgcgttcg cccgtagctt cgacctcctc
     1621 ctgacgctgc gcttcttgca gggtatgggg gccgccgccc ttggcaccct gaacgttacc
     1681 gtgataggcg acatctacga cggccgcgag agatccgcag ccctcggata caactcgagc
     1741 gtcctctccg tcgggacggc gagctacccc gcgatcggcg gtctgctggc gacattcggg
     1801 tggttctatc ctttcgccct gcccttcgtg gcgataccta tcgggataat cgtactgttc
     1861 tcgctgcgca atccggagcc gcgcaacgac gatcggctga aggactactt cgggagcgtg
     1921 tgggagaatc tccgcgaccg tgaggtcttc ggacttatcg gcgcctcact tctgacgttc
     1981 atcgtgctgt tcgggccgca gatctcatac ctcccgatcc tgatgaacgc gcgcttcgac
     2041 gcttcttctt tcgtgatcgg agccgtcctc tccggagcct ccctgactac cgcgcttaca
     2101 tcgacccaac tcggccgtct gactctacgc ttctccgaga gaagcttgct gaagtcggct
     2161 ttcgtactgt atgcgatcgc cctggttgcg gtcgcgttca ctccgagcct gcccatgctc
     2221 ctcgtccccg ccgtcctttt cggcgtggcg caggggatca acctccccaa cgtcttctct
     2281 ctgctcaacg cccacgcccc aacagagaac cgcggagctt ttatggcggc gaacggcatg
     2341 tccctccgca ccggacagac gatcggaccc ctcttcatgg cctacgccgc aggaacgatc
     2401 ggcctgacag gtgcctacct gtccgccgcc ggcctcgcca tcctcgcctt cttcctcgtc
     2461 ctcacactcg tgcgctaggg tccggcctcc acttttcacc acttcagctc tgaccgcttt
     2521 gaatagcctc tcatttttgc cagccacatc taccgtggcg ctccggatgg cggtgtgtta
     2581 tcttacagtg taagtttgag gtctggcggg atcgaatcca caggggtggt gatcgtggga
     2641 atgagtgggt gggaggggag tttgggagga tctgtgcggc gtggaatata cgcgcggcac
     2701 aggttggatc tctctgtggt agatatcctg ttcggggttc tctcgtgtgg ccgtctatgg
     2761 gagagggagc ggctggaggc ggaggtcctg ggcctctgtt cactagaagt cgaaggactg
     2821 gtctgtttct ctgtgcggag cgggtgggac ctgtggctcg gggcgctggg tcttcgggct
     2881 ggagatgagg tcctcgtcac ggccgtcacc catcctgaca tggtcaggat catacatgga
     2941 cacgggctga gggccgtccc ggtggacatc gacccggaga cgctcgcgcc gcgtcccgag
     3001 atgctggagg cggcactcac gccgcgcacg cgggtcgtgc tcgtcgccca cctcttcggg
     3061 ggacgtatgg atctcggacc cgtggccagg gtcgcgaggg agcatggtct gctcctcgtc
     3121 gaggactgtg cccaggcttt cagggggccg gagagcctgg gggaggcgga gtccgacctg
     3181 tccatgtaca gtttcggcac cctcaagaca gcgaccgcgc tcggaggcgc ggttctacga
     3241 gtacgagatc ctgaagtcct tcgaaaaatg cgtgagttcc aggccgccta tcctgtgcag
     3301 cgcaggagcg agtatctgaa gaagctgctc aaggtcctgg tcctcgcagt ggtctcacgt
     3361 cctcgaccct acgggctgtt cgtcctggca tgcacgcggc tcggccacga cctggacgcc
     3421 ctggtcaacg gcgtggtccg agcctttccg cccggggagc cgatcgagaa gctcttccgg
     3481 cggctgagac gcaggccttc cacgccgctg ctcgctatgc tctcccggcg gcttgggacg
     3541 ttcgatgagg tcaggctcgc caggagggcc tctgccggcg agcgtgcggc gcgtagactg
     3601 cgggtggccg gtgtgcatcc ggggaggaat tcattacagc cgacgcactg gctcttcccg
     3661 gtcgttgctc ctgaccctgg ggctttgatc tcgggcctcc gaaggcgagg cttcgacgcg
     3721 agccgggtta cgagcaacat cgccgtcgtc gaagctcctg tcgggtgttc gtcagcggcc
     3781 gaagcttccc ggatgatgtc aggaatcgtc ttcctgcctg tatgccagga tctcccttca
     3841 caagcctccg atgccatggt cgatctcgtc aacgaatgta ccgtggctga accggtcgag
     3901 gatctagcgc ggccatgagc gaggttcggg aacgcacgat ggaggaactg gaactcggag
     3961 tgttcgacgt gctcgtgttg ggcggcggca tagtcggggg ccgtgtggca ttcgacgctg
     4021 cgcgggccgg tcttagggtg gcgctcgtcg acgcagggga cttcggtggg gcgacc
//
LOCUS       DATAIR010000504         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_309911, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000504 DATAIR010000000
VERSION     DATAIR010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309911"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="VHM69_12055"
     CDS             <1..1901
                     /locus_tag="VHM69_12055"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX2741173.1"
                     /translation="QTIDRIEKRDDTLAITGRLTNGEDPEGIGYTLTFSPVTGGRLRF
                     EAEVEKPYNRVYLTYASSPDERFFGFGTQYTYFDMKGRMVPIFIQEQGIGRGKQPITW
                     AVDWKAGAGGTPYTSYASVPHYITSEMRSLFLENYEYSSFDLRKEDRVQIGVFSSRMR
                     GQILSGETPAELIGQYTEYSGRMRPLPEWVLGGAVVGLQGGADRVLQMSDRLDSLDAP
                     VAALWLQDWVGQRQTSFGTQLWWNWELDEDHFPSWDSLRQHLEIRNIRLITYINPFLC
                     DDVQNHRRNLFEEATGKGYLVTSREGEPYTIKISSFSAALVDLTNPEARTWIKGIIRD
                     ELIGNGASGWMADFGEGLPYDAVLHSGADPKSYHNRYAEDWAEVNREAISEAGREDDI
                     VFFNRSGYTKSPRYSTLFWLGDQLVDWDEHDGIKSAVTGLLSSCLSGYSLEHTDIGGY
                     TAIDSALLKYHRSKELLMRWIELGAFTTVFRTHEGNRPEVNHQIYSDEETFRHFIRFA
                     KIYAAWKPYRMELVREASETGLPVVRHPFIHYPRDPEILGLNYQFMVGTEFMVAPVLD
                     PGEENVRIYLPAGTWIHLWTGNTYGSSERGVYETVSAPIGEPAVFYKEGTAEGRRLRE
                     DLSSKGLL"
     gene            1955..2362
                     /locus_tag="VHM69_12060"
     CDS             1955..2362
                     /locus_tag="VHM69_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2741174.1"
                     /translation="MKRENVSSGTVWESTVGYSRAVRRGPFVFVSGTTATDGSGGYVG
                     VGDPYAQTVQAINNIKVALQAVGADLSDVVRTRIYVVNIEDWEKVGEAHGECFGKVRP
                     ATTMVEVSRLIAPEVMVEIEADALIDAEYRRSM"
     gene            complement(2377..>3009)
                     /locus_tag="VHM69_12065"
     CDS             complement(2377..>3009)
                     /locus_tag="VHM69_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741175.1"
                     /translation="YLFPVNPERLVERAAAPLFEELSAVLEEIAAVLESGDGERAERV
                     LQRARDIDDRVRTLIEALEAGHETARLSPTRRRSLRQLEFYGSIGIRIELAVINTRVL
                     ARGVYNAIRRGDNIAPQLPEAVRDLARSVKALAAFLEDGEGPEEARRWALEAARRATE
                     ILDERHDLGISVLVGQVRSAAVDLLRSTGMDQASALRALEDAAGRASEIG"
BASE COUNT          609 a          895 c          955 g          550 t
ORIGIN      
        1 accagaccat agatcgcatc gagaaaaggg atgatacgct cgccatcacc ggacggctca
       61 cgaacgggga agaccctgaa ggcatcggct acacgctcac cttctcacct gttaccggtg
      121 gaaggctacg gttcgaagcc gaggttgaga agccctacaa ccgggtctac ctgacctatg
      181 cttcgtcacc ggacgagcgc ttcttcggct tcgggacgca gtacacctac ttcgacatga
      241 agggccgcat ggtgcccatc ttcatccagg agcaggggat aggacggggc aagcagccca
      301 taacgtgggc ggtcgattgg aaggccggcg caggcggcac cccgtacacc agttacgcca
      361 gcgtgccgca ctacataacg agcgagatgc gttccctgtt cctggagaac tacgagtact
      421 cgtccttcga tctcaggaag gaggacaggg tccagatcgg ggtcttctca tcgcggatgc
      481 ggggtcagat cctgagcgga gagacccccg cggagctgat cggacagtac accgaatact
      541 caggcaggat gcgcccgcta cccgagtggg tcctcggcgg cgcggtagtg ggattgcagg
      601 gcggcgcgga tagagtgctc cagatgtcag acaggctcga ttccctcgac gcacccgtcg
      661 ccgccttgtg gctgcaagac tgggtggggc agaggcagac tagcttcggc acacagctct
      721 ggtggaactg ggagctggac gaagaccatt tcccaagctg ggactcgttg agacagcacc
      781 tggaaataag aaatatcagg ctcattacct acatcaaccc gttcctctgt gacgacgtcc
      841 agaaccaccg ccgcaacctc ttcgaggagg ctacagggaa agggtacctg gtgacgagtc
      901 gggaaggtga accctacacg atcaagatct ccagcttctc cgctgccctc gtggatctga
      961 cgaacccgga ggcgcggacc tggatcaagg gcattatcag agacgaactt atcggtaacg
     1021 gggcctcagg gtggatggcg gacttcggcg agggacttcc ctacgacgcc gtgctccact
     1081 ccggcgctga cccgaagagc taccataacc gctacgccga ggactgggcc gaggtgaacc
     1141 gcgaagccat cagcgaggct ggccgtgagg acgacatcgt cttcttcaac cgttccggtt
     1201 acacaaagag cccccgctac agcaccctgt tctggctggg ggatcagctc gtcgactggg
     1261 acgagcacga cggcataaag agcgccgtca cgggcctctt gtcgagctgt ctctcaggat
     1321 atagcctcga acacaccgac atcggtggct acacagccat agacagcgct ctgctcaagt
     1381 accatcgttc aaaagagttg ttgatgcgct ggatcgagct cggcgccttc accacggtgt
     1441 tccgtaccca cgagggcaac cgacccgagg tcaaccacca gatctactcg gatgaagaga
     1501 ccttcagaca cttcatccgc ttcgccaaaa tctacgcggc ctggaagcca tacaggatgg
     1561 aactcgtcag ggaagcgtcc gagacgggcc tccctgtcgt caggcatcct ttcatacatt
     1621 atcctagaga tcctgagatt ctcggtttga actaccagtt catggtaggc actgagttca
     1681 tggtggcgcc ggtcctggat cccggcgaag agaacgtaag aatctacctg cctgcgggca
     1741 catggatcca tctgtggaca ggaaatacgt acggctcgtc cgagcgcggc gtttacgaga
     1801 ccgtttcggc tcctatcgga gagcccgccg tcttctacaa ggaaggcact gccgaaggac
     1861 gacgtttaag ggaagatctc tcaagcaagg ggctgctgtg acagcgggaa ggtagaatgg
     1921 atcggcgcga ttcgttgtgg ccggaggggc tctgatgaag cgggagaacg taagcagcgg
     1981 cactgtgtgg gagagcacgg tcggctactc gcgggcggtc aggcgcgggc ctttcgtgtt
     2041 tgtttcagga accacagcca cggacgggag tgggggctac gttggggtcg gtgatcccta
     2101 cgcgcagacc gtccaggcca taaacaacat caaggtggcg ctacaagctg tcggggcgga
     2161 tctctccgac gtggtgcgga ccaggatcta cgtggtcaac atcgaggact gggagaaggt
     2221 cggggaggca cacggcgaat gcttcgggaa ggtcagaccg gccacgacca tggtcgaggt
     2281 cagccgcctc atcgcgcccg aggtcatggt cgagatcgaa gccgacgccc tgatcgacgc
     2341 ggagtacaga cgctccatgt aggggcggga accgccctac ccgatctccg aggcgcgtcc
     2401 ggcggcgtct tcgagcgctc gcagggccga tgcctgatcc atccccgtgc tcctgagcag
     2461 gtcgacggcg gcggagcgga cctggcctac gaggacgctg atccccaggt cgtgtcgctc
     2521 gtcgagtatc tcggtagcgc gcctggctgc ctcgagcgcc caccggcgtg cctcctcagg
     2581 tccttcgccg tcctccagga aggccgccag ggcttttacg gagcgcgcga ggtcgcgtac
     2641 tgcctcgggt agctgcggtg cgatgttgtc tccgcgtctg atcgcgttgt aaacgccgcg
     2701 ggccaggacg cgggtgttta tgacagcgag ctctatcctg atgccgatgc ttccgtagaa
     2761 ctcgagttgc ctgagggacc tgcggcgggt tggggagagt ctggccgttt cgtggcccgc
     2821 ttcgagcgcc tcgatcaggg tcctcactct atcgtctatg tcccttgccc gttgaaggac
     2881 tctttcggcc cgttcaccgt caccgctctc gagcacggcg gcgatctctt cgagcaccgc
     2941 ggaaagctct tcgaagagcg gggccgcggc cctctccacg agccgctcgg ggttgaccgg
     3001 gaagaggta
//
LOCUS       DATAIR010000505         4662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_153679, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000505 DATAIR010000000
VERSION     DATAIR010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153679"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VHM69_12070"
     CDS             complement(<1..715)
                     /locus_tag="VHM69_12070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2741176.1"
                     /translation="MRKPAQDGVRTREVALWAAMAFFLLAQGLATLLFVRHGWDALTF
                     PYPLNYGEGPILDQSVRLAHFQNIYRTDLSGPPYVVSNYPPLFMIVQVPFVWLFGPAL
                     WYGRLISLLSVAAVALFVGLTLHALTRDRIAAVVGGLIFPCMPYVVRWSSLSRVDSLG
                     LALSWAGLFVVARWPERRWSVFISALFLVAAIYTRQTYALAAPFAAFVWLVAQGQRRR
                     ALETAAIAGGATLLMFAFLN"
     gene            923..2554
                     /locus_tag="VHM69_12075"
     CDS             923..2554
                     /locus_tag="VHM69_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2741177.1"
                     /translation="MERLGESARDLTEGVEDSEQFRIDAKDFLSARERGGTTGTRIDQ
                     QTAALADAAEIVNRSFPTLEPSGRFINAISPVVIPNRQSLFWRLMPIGMMVVFLFLGA
                     IVTLAIAGLAGGGVALALFGPHLWLLLVLLAFFSWWRQSVVMVPDGCKAVITRFGKLE
                     EVVEAGRKLLFNPWKQVSYIVNTTREYPYNAPIRQAPTASRVNASVDLFLQFRIEDPA
                     EFIFTVGGVKGFSDKLENAISEVTRALIYEQRAEDIYDLVGESTQKFLDSLNEQFLPA
                     VRFTNANITHAEPSSQEYRMDLAAPEMVRVAKEAYTYEYELRLRKEQDEGELRGELAS
                     LRETLSAISAEIATYQAQIDTARERETNRANASARERMVEAESTANANAALLEAQALD
                     IRAVSSAAAPEILEYRFQQDVLERLAAVADRLPQVVQIGAIDGDDAVDFLEISRRMIG
                     TGGEPLFTPEDMQAIRDRMEEIQARIRERAIELESLSDGHETRPEEEPAAEEATADRV
                     EEIRQSVTDEAVRERVERIGHDGQPDGGANGEEGR"
     gene            2551..3888
                     /locus_tag="VHM69_12080"
     CDS             2551..3888
                     /locus_tag="VHM69_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2741178.1"
                     /translation="MTETMESMQAQLQTQTGARVQEFSRITEVVSGWSGVRQYLRGGD
                     AGQIVPVVIPKDRRRYGWLFLLGVAIYMLGFAVFSGSAVLAILAALVAIFFLGLALLW
                     LWRGAIVEIEQGTTGVRSRYGRIEGTLSPGRHYLWRPWDKVEFVVDTATEIPYLAPVA
                     VCPTRENVPLKAIEFFLKFRIVEPLAFVRVIGASNFDLVLSSAVQDAIRQRSRQVSTE
                     KAYDLRGSDVGDMQEYLNRQLAQYGVRILGANIPNVELPDQYQQHLATRERVAKELAA
                     YEREWELTRKQRIDTLLMEIERSKKDRDARLIEVKEARNRARQDVARMLEEQETEAQR
                     VRLEIETRGRAALTEAENEATALRHLGQAHRDNRAVVRYELARRRVEVGERLMQRAPR
                     PVLIRGEGGEQSALSTLILARLLPRLTEDGDDALKGAGNSLRDTVRDLGDGEG"
     gene            complement(3975..>4662)
                     /locus_tag="VHM69_12085"
     CDS             complement(3975..>4662)
                     /locus_tag="VHM69_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2741179.1"
                     /translation="ADEGAEVIVVEGTYDDAVEISAEERGLLISDMSWPGYERIPSWV
                     IEGYSTMLWEVDDELERRNEAGPDLVVVQVGVGAFAAAVARHFRGPGATRPKLIAVEP
                     TSADCLFQSVAAGRIVSVPGPHDSIMSGLNCGRPSLAAWPTVSRGIDLLVAVDDGPAR
                     EAMRLAAESGIVSGETGAAGLGGLLELLRADAGEEARQALGIDKESRVLLFNCEGATD
                     PGAYQRVLEE"
BASE COUNT          906 a         1473 c         1546 g          737 t
ORIGIN      
        1 tgttcaggaa cgcgaacata agcagggtgg cgccgcctgc gattgcggcc gtctcgagcg
       61 ccctgcggcg ctggccctgg gccacgagcc acacgaaggc cgcgaaaggt gccgccaggg
      121 cgtaagtctg gcgtgtatag atggccgcca ccagaaacag ggccgagata aagacgctcc
      181 atcgcctctc cggccagcgc gcgacgacga aaagacctgc ccacgacagc gccagcccga
      241 gcgagtcaac cctggacagc gaagaccagc gcaccacgta gggcatgcaa gggaagatca
      301 ggccccccac caccgcggcg atgcggtctc tggttaacgc gtgcagtgtc agccctacga
      361 agagcgcgac ggcagcgacg ctgaggagtg agatcagacg cccgtaccac aacgccggcc
      421 cgaagagcca cacgaaaggc acctggacga tcatgaagag cggggggtag ttggagacga
      481 cgtaaggcgg cccggagagg tcggtcctgt agatgttctg gaagtgggcg agccttacgg
      541 attggtcgag gataggtccc tccccgtagt tgagcgggta ggggaaagtg agcgcgtccc
      601 atccgtgccg cacgaagagc agcgtcgcga gaccctgcgc caggaggaag aaggccatgg
      661 cggcccagag cgcgacctct cgcgtccgca caccatcctg ggcaggcttt ctcattttct
      721 ccgtgctcgt cgccgactcc ttccacgttc tgtggcgcag gtgttgtatc ctaccgcttt
      781 ggcgtcacca gcgggcaata cgtgccgcct tcaggcgggt tccgtatggg cagcgggcct
      841 ggatcagggt tgtgctgtgg ttgggggcgt gcaagtatga ggacataggt gatgtcatcc
      901 tagaatcctg gaggattatc tggtggagag gttaggggag agtgcacggg atctgacaga
      961 gggggtagaa gactccgagc agttcaggat cgacgcaaag gactttctct cggcgcggga
     1021 gcgcggcggt accacgggga cgcgaattga ccagcagacg gcggccctcg ccgacgcggc
     1081 cgagatcgtc aaccgtagct tccccaccct ggagccatcg gggaggttca taaacgcgat
     1141 ctcacccgtc gtcatcccga accgccagtc gttgttctgg cgtctgatgc ctatagggat
     1201 gatggtcgtg ttcctcttct tgggcgctat cgtcacgctc gcaatagcag gactcgccgg
     1261 cggcggggtg gcgctggcgc tgttcgggcc tcacctctgg ctgcttctgg ttctgctcgc
     1321 cttcttctcc tggtggcgcc agagcgtcgt gatggtccca gacggctgca aggccgtcat
     1381 cacccgcttc gggaagctcg aggaggtcgt cgaagcaggc cgcaagctgc tcttcaaccc
     1441 gtggaagcag gtcagctaca tcgtcaatac gacgagggaa tatccgtaca acgcccccat
     1501 aaggcaggca ccgacggcga gccgggtgaa cgccagcgtc gaccttttct tgcagttcag
     1561 gatcgaagac cccgcagagt tcatcttcac cgtcggcggg gtcaagggtt tctcggacaa
     1621 gctggagaac gccataagcg aggtcacgcg cgccctgatc tacgagcagc gcgccgagga
     1681 tatctacgac ctggtaggag agagcaccca gaagttcctg gactccctga acgagcaatt
     1741 cctccccgcc gtgcgcttca cgaacgcgaa cataacccac gccgagccat cgagccagga
     1801 gtacaggatg gacctcgcag cccccgagat ggtcagggtg gcgaaggaag catacaccta
     1861 cgagtacgag ctcaggctgc gaaaggagca ggacgagggc gagctacggg gcgagcttgc
     1921 gtccttacgc gagacgctat cggcgatcag cgcggagata gcgacctacc aggcccagat
     1981 cgacacggcc cgcgaacgcg agacgaaccg cgccaacgca agcgcccgcg agcggatggt
     2041 cgaggcggag agcacggcca acgccaacgc tgccttactc gaagcccagg ctctcgatat
     2101 tcgggccgtc agcagcgcag cggctccgga gatcctggag taccgcttcc agcaggacgt
     2161 cctcgaacga ctcgccgcgg tagcagacag gttgccgcag gtcgtccaga tcggggccat
     2221 agatggggat gacgccgttg atttcctgga gatctcgcgc cgtatgatcg ggaccggcgg
     2281 cgagcctctg ttcacgcccg aggacatgca ggcgattcgg gaccgcatgg aggagataca
     2341 ggcccgcatc cgcgagcgcg caatcgagct cgaaagcctc tcggacggcc atgagacacg
     2401 tcccgaagag gaacctgccg ccgaagaagc gaccgcggac agggtcgagg agatccggca
     2461 gtccgtaacc gacgaggcgg tgcgcgagcg cgtcgagcga atcggccacg acggacagcc
     2521 agatggcggc gccaatgggg aggagggacg atgaccgaga cgatggagtc gatgcaggcg
     2581 caacttcaga cgcagactgg agccagggta caggagttct ccaggataac ggaggtcgtc
     2641 tccggctggt ccggagtcag gcagtatttg aggggcgggg acgcggggca gatcgtccca
     2701 gtcgtcatac cgaaggaccg caggcgctac ggatggctgt ttttgctcgg cgtggcgatc
     2761 tacatgctcg ggttcgctgt gttctcgggg agcgcggtgc tcgcgattct ggctgcactg
     2821 gtcgcgatct tcttcctcgg tctcgcgttg ctgtggttgt ggcgcggcgc catcgtcgag
     2881 atagagcagg ggacgacggg tgtgcgctcg cgctacggga ggatcgaagg caccctctcg
     2941 cctggccgcc actacctctg gcggccttgg gacaaggtcg agttcgtcgt cgacacggcg
     3001 accgagatcc cttacctcgc ccccgtcgcc gtctgcccga cgcgggagaa cgtgcccctg
     3061 aaggcgatag agttcttcct gaagttccgc attgtagagc cgctcgcctt cgtacgcgtc
     3121 atcggggcct cgaacttcga cctcgtcctc tccagtgctg tgcaggatgc gatccgtcag
     3181 cgtagccgcc aggtgagcac ggagaaagca tacgacctgc gcggcagcga cgtgggagac
     3241 atgcaggagt acctgaaccg acagctcgcc cagtacgggg tccgcatcct cggcgccaac
     3301 atcccgaacg tggagcttcc cgaccagtac cagcagcacc tcgctacccg cgagcgcgtc
     3361 gccaaagagc tcgcagccta cgagcgcgaa tgggagctca cccgcaaaca gcgcatcgac
     3421 acgctcctca tggagatcga acgctccaag aaagaccgcg atgcccgcct catcgaagta
     3481 aaggaagccc gcaaccgcgc ccgccaggac gtcgcccgca tgctcgagga gcaggagacc
     3541 gaagcccagc gcgtgcgcct cgaaatagag acgcgtggcc gcgccgccct gacggaagcc
     3601 gagaacgaag caacggccct ccgccacctc ggacaggccc acagggacaa ccgcgccgtc
     3661 gtccgctacg agctggcccg tcgcagggtc gaggtaggcg agcgcctcat gcagcgcgct
     3721 ccgcgtcccg tcctcatccg cggcgaaggc ggcgagcagt ccgcgctctc caccctcata
     3781 ctagcccggc tcctcccgcg gctgaccgag gacggggacg atgcgctgaa gggcgccggc
     3841 aactccttgc gcgacactgt gagggatctc ggagacggcg aaggttagta aagtcgagac
     3901 gcccgacgcg ggctcagttt gagagaggcg ctcttggcac agacgggtgt tatctgaccc
     3961 tggcaggtgg gtggctactc ctccaatacc ctctggtatg caccggggtc ggtggcgccc
     4021 tcgcagttga agagcaggac gcgggactcc ttgtctatgc cgagcgcctg gcgtgcttcc
     4081 tcccctgcgt cggcgcgcag aagctccagc agaccgccga gcccagccgc cccggtctcg
     4141 ccagacacga tgccagattc cgccgcgagg cgcatggcct cgcgtgccgg accgtcgtca
     4201 acggcgacga ggaggtcgat gcccctggat acggtcggcc aggcggccag tgaaggtctg
     4261 ccgcagttga ggccggacat gatggagtcg tgcggaccgg gcacggaaac gatgcgccca
     4321 gccgcaaccg actggaacaa gcagtcggcg ctcgtcggct cgacggctat gagtttcggg
     4381 cgcgtagctc cggggcctcg gaagtggcgg gccacggccg cggcgaaggc gcctacgcca
     4441 acctgcacga ccacgaggtc gggtcccgct tcgtttctcc tttcgagctc gtcgtcgacc
     4501 tcccacagca tcgtcgagta gccttcaatc acccatgaag gtatacgctc gtagccgggc
     4561 caggacatgt ccgagatcag gagcccacgt tcttcagccg agatctcgac ggcgtcgtcg
     4621 tatgtacctt cgaccacgat gacctcggcg ccctcgtctg cg
//
LOCUS       DATAIR010000506         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_199617, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000506 DATAIR010000000
VERSION     DATAIR010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199617"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VHM69_12090"
     CDS             complement(<1..1084)
                     /locus_tag="VHM69_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2741180.1"
                     /translation="MTMGGSFKIGRAFGIDVKVHWSFFLLLAFFGFLAYRDSGSVASA
                     LVTVGIVVALFFCVLLHEYGHSLVAIRLGSEVQDITLLPIGGLARMKSLPERPIDEVK
                     IAVAGPLVNVVLAPVFYGLALLFGGSLSVPTDILSGASSLGQVFAALGTINVALVVFN
                     MIPAFPMDGGRVLRGLLATRLGQVRATDISSTIGQAFAILFILLGFFGNNFLLAIVGF
                     FVFLGASGEAQLVRQKEQMRGLAVADVMGTKGRTETVTPYHNFGQILDSVIHGYQVDF
                     PVVDEEGRLVGIITRNEIMSAAHSPARFSSVRDLMKTDFPTISPDADLFTEGQRVLRE
                     SGLRALPVVKDGNLVGMLTIEDVGQAA"
     gene            1150..>3707
                     /locus_tag="VHM69_12095"
     CDS             1150..>3707
                     /locus_tag="VHM69_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX2741181.1"
                     /translation="MEERTESYRLVTAAEDLSAVAKTIQDAEVIGVDIETTGLSPRDG
                     RLRLLQIATPGETFIIDAFEVVDLSPLKDALEDGPVKILQNCVPLDTDVLTPSGWRPI
                     STIRRGDTVMGYENGEQRWTRVAEYINGGVQEVIESGNSRRHWRSTANHRWVCERRYG
                     KRARLRREEYLTTRGLSESVTNSVRVKHSAPFVNPTPVAVSEEEAGLLAWLWGDGHVG
                     RRQCSITQSENNRDSCEKIEALLDSLGVERRFTRLPESPHIRRYYVPTDFVRSVYARA
                     RMSFDSPDYEAFVLDLSFEALESWFEHFWYAEGSKSRPFPQVVCQTRKADTDKYEALL
                     LAGHLLGYTPIPGEPDFLSFGRPFTALRKVNHASVGFEKVACLTTETSNFVMRQGDFV
                     TITGNSKFDYQFLHAQHGISLSPVFDTMLAAQLLDGGDYSASYALEAIAERYLDEAVD
                     KSERRADWSGELGEAQLEYAARDATVLLPLRERLAELLEKEGLGRISRIEFGAVSTIA
                     EMELAGVKLDVARWKELEVKVRERRDRAAERLEAFFPPPEGVLPLEGLGPRLNLNSPK
                     QITDAFRSLGIELPDTKVWTLLKVDHPAAKALLEYRELQKKLGTYLETYPGFIHPKTG
                     RIHANFLQCRVPTGRLACTNPNIQQIPHEDEFRSCFVAEEGNTLVIADYSQIELRILA
                     EVSDDPAFVTAFQRGDDLHRLTAATMYNVSMEEVTRDQRTAAKRINFGLMYGRGARSL
                     SAQLGTDEERGRQLIDEYFANYPKVQRFLQRTANRAMRDRTLRTLAGRVRKFGNNPVA
                     DDRGAMRREAMNYPIQGAASDIAKLALIYVREDLAELDARLINTIHDEFVVECS"
BASE COUNT          771 a         1124 c         1173 g          639 t
ORIGIN      
        1 gggcggcctg gccaacgtct tctatcgtaa gcatcccgac caggttgcca tccttcacga
       61 cgggcagggc cctcaggccg ctctcccgca ggacgcgctg gccctccgtg aagaggtccg
      121 cgtcgggcga gatcgttggg aaatccgtct tcatcaggtc gcggacgctg gagaagcggg
      181 caggagaatg ggcggcggac atgatctcat tgcgggtgat gatgccgacg agccttcctt
      241 cctcatcgac gacggggaag tccacctggt agccgtggat gacggagtcg aggatctggc
      301 cgaagttgtg gtatggcgtc acggtctcgg ttcggccctt ggtacccatg acgtcagcca
      361 cggcgagccc gcgcatctgc tccttctggc ggaccagttg agcctcgccg ctggcgccga
      421 ggaagacgaa gaagccgacg atggcgagca ggaagttgtt gccgaaaaac cccaagagga
      481 tgaagagaat ggcgaaggcc tgtcctatgg tcgatgagat gtccgtggcg cgcacctgcc
      541 ccaggcgggt tgcgagcagg ccgcgcagca cgcggccccc gtccataggg aaggcgggga
      601 tcatgttgaa gacgacgagg gcgacgttga tggtgcctag cgcggcgaag acctgcccca
      661 gggaacttgc ccccgagagt atgtcggtcg gaacagagag gctgcccccg aagagcagtg
      721 ccagcccgta gaagacaggg gcgagcacga cgtttacaag cggacctgct acggcgatct
      781 tcacctcgtc gatgggcctc tcgggcaacg atttcatcct ggcgagccct ccgatgggca
      841 ggagcgttat gtcctgaacc tcgctcccga gacgtatggc cacgagcgag tggccgtact
      901 cgtgaagcag gacgcagaag aatagcgcca ccacgatgcc tacggtgacg agcgcgctgg
      961 cgacgctgcc agaatccctg taggcgagga agccgaagaa tgccaggaga aggaagaagc
     1021 tccagtggac cttcacatct atgccgaacg cgcgtcctat cttgaacgat ccgcccatcg
     1081 tcatgagttt accccatcaa cgtgcgggag aagtgatccc acagacccat cccctgctag
     1141 aatcgaaggg tggaggaacg caccgaaagc taccgtctcg ttactgctgc ggaggacctc
     1201 tccgccgtcg cgaagacgat ccaggacgcc gaggtcatcg gcgttgacat agagaccacg
     1261 ggcctgagtc cccgtgacgg caggctgcgc ctcttgcaga tagccacgcc cggggagacc
     1321 tttatcatcg acgcgttcga ggtcgtggac ctctcgcccc tcaaggacgc tcttgaggac
     1381 gggccggtca agatcctgca gaactgcgta cctctggaca cggacgtcct gaccccttcg
     1441 ggttggaggc cgatctctac gattcgacgc ggtgacaccg tgatgggcta cgagaacggc
     1501 gagcaaaggt ggactcgggt cgcggagtac ataaacggcg gcgtgcaaga ggtgatcgaa
     1561 tcaggaaact cccgcaggca ttggcgctcg actgcgaatc acaggtgggt ctgcgaaagg
     1621 agatacggaa agagagcccg tttgcggcga gaggaatacc tgaccaccag ggggttgtca
     1681 gagagtgtta cgaactccgt gcgcgtcaag cactccgcgc cgttcgtcaa cccgacaccg
     1741 gtcgcggtgt ctgaagaaga ggcaggcttg ctggcctggc tctggggcga cggtcacgta
     1801 gggaggaggc agtgctcgat cacccaatcc gagaacaatc gggattcttg cgagaagatc
     1861 gaagccctgc tcgactcgtt gggtgtcgaa cgtcgtttca cgcgacttcc tgagagtcct
     1921 cacatccgac ggtactatgt ccctacggat tttgttcgga gcgtctacgc cagagccaga
     1981 atgtccttcg attcaccgga ctacgaggcg ttcgtgctag acctttcgtt cgaagcgctg
     2041 gagtcgtggt ttgagcactt ctggtacgcc gaaggaagca agtcacgtcc gtttccgcag
     2101 gtggtatgcc agaccaggaa agctgataca gacaagtacg aggcattact gctggcagga
     2161 cacttgctag gatatacgcc gattcctggg gaaccggatt ttctaagctt cggacgacct
     2221 ttcaccgcgt tgcgaaaggt caaccacgcg agcgtcggtt tcgagaaggt ggcgtgtttg
     2281 accacggaga cctccaactt cgtaatgcgt cagggagact tcgtgacgat caccgggaac
     2341 agcaagttcg actaccagtt cttgcatgcc cagcacggga tctccctctc tcccgtcttc
     2401 gataccatgc tcgctgcgca gctactggac ggcggcgatt actccgcatc ctacgcgctc
     2461 gaggcgatag ccgagcgcta cctcgacgag gcggtggaca agtcggagcg gcgcgcggac
     2521 tggtcggggg agttgggcga agcccagctc gagtacgctg ccagggacgc tacagtcttg
     2581 ctgccgttgc gcgagcgtct cgctgagttg ctcgaaaaag aggggcttgg gaggatctca
     2641 cgcatagagt tcggggcggt cagcacgatc gcggagatgg agctcgctgg cgtgaagctc
     2701 gacgtggccc gctggaagga actagaggtg aaggtgcgcg agcggcgcga cagggccgcc
     2761 gaacgcctgg aggctttctt cccgccgccc gaaggggtgc tgccgctgga gggcctgggg
     2821 ccgcggctca acctcaacag tcccaagcag atcaccgacg ccttccgcag cctgggcata
     2881 gagctccctg acacgaaggt ctggaccctc ctcaaagtgg accaccccgc ggcgaaggcg
     2941 ctgctcgaat accgcgaact gcagaagaaa ctcggcacct acctggagac ctaccctggc
     3001 ttcatacacc cgaagacagg ccgcatccac gcgaacttcc tccagtgccg cgtcccgaca
     3061 gggcgacttg cctgtacgaa tccgaatatc cagcagatac cccacgagga cgagttccgc
     3121 tcgtgcttcg tcgccgagga gggcaatact ctcgtcatag cggactactc ccagatagag
     3181 cttcgcatcc tggccgaggt ctccgacgac cctgccttcg tcaccgcctt ccagaggggt
     3241 gacgacctcc accgcctgac agcggccacg atgtacaacg tgtcgatgga ggaggtcacc
     3301 agggaccagc gcacggcggc caagcgtata aacttcggcc tgatgtacgg ccgcggcgcc
     3361 cgtagcctct ccgcccagct cggcacggat gaggaacgtg gccggcaact tatagacgag
     3421 tacttcgcca actacccgaa ggtccagcgc tttctgcaga ggacggccaa ccgcgccatg
     3481 cgggaccgca ccctccgcac gctcgccgga cgggtgcgaa aattcggcaa caaccccgtg
     3541 gccgacgaca ggggcgccat gcgccgcgag gcgatgaact atccgattca aggggcggct
     3601 tccgacatag ccaagctggc cctgatctac gtccgcgaag atctcgccga actggacgcc
     3661 cgcctcataa acaccatcca cgacgagttc gtcgtcgagt gttccga
//
LOCUS       DATAIR010000507         8707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_32792, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000507 DATAIR010000000
VERSION     DATAIR010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32792"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..376)
                     /locus_tag="VHM69_12100"
     CDS             complement(248..376)
                     /locus_tag="VHM69_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741182.1"
                     /translation="MEWFAVVEWFGRPEIETDEAREHRISMEAVVDAYNEQERAIG"
     gene            complement(528..704)
                     /locus_tag="VHM69_12105"
     CDS             complement(528..704)
                     /locus_tag="VHM69_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741183.1"
                     /translation="MAEREPEATLEPTRGRLAPGADGTPVVAPAILALPAIPAAGSPR
                     DDQMITTLQYVARI"
     gene            938..3352
                     /locus_tag="VHM69_12110"
     CDS             938..3352
                     /locus_tag="VHM69_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2741184.1"
                     /translation="MPTGRAQTPNFPGIAADRRGDGRPTTWAGVASKGNLPLELSSFV
                     GREREISQVRGVMGDARLLTLTGPGGCGKTRLALRVAADLAGSGERFGDGAWWVDLSS
                     LSDPSLVPSTVASAFGVREAPDRTPTEALAAFLEAKSPLLVLDNCEHLVEACATLGDV
                     LLRSCSGLKIMATSREPLGVAGEISWPVPPLALPDPNRRLNAQDLLRYSAVRLFVERA
                     RAASFGFSFTEENASVVAALCARLDGMPLAIELAASRVRVLSLRQILERLDDRFRLLR
                     GSRTSVPRHKTLEATIDWSHELLSEEEKILFRRLSVFAGGWTLAAAEEVCADDGIAED
                     EILELLSSLVDKSLVLASQVEGSGEAHYRMLQTIQQYAAQRPGEPGDAEAVGRRHAEY
                     FLSLAERAEPALAGPEQAAWMERLEEEHDNLRAALGWFEEKGEAERGLRLAAASLRFW
                     WLHGHLSEGRTYLETLLDRPGAQVRDEVRAEALQVLGRMIYLHADYIDGGWEVARCRL
                     EESLRIYRRLGNERGVADVRQSLGRVYPELGERRAAFTFLEESLEIERRLENGSGIAR
                     SLFNLGYARLLDGDLPSARSYFEEGLRIFRKLDDGFWINASLVHLGFIDCEEGELARA
                     RAKFIHMNEVLPLVQFPWGTTYTLEGFARLAAAEGQPVRALLLGGATAELRRTFGVAI
                     GPTRRAGFRRSLKPAWQALGDEDGKAAWDKGRTMTLEEALALALEEPEKKPDRPPGSI
                     LSAREVEVLSLVAEGLSDAEVAKMLYISPRTVGNKLHGAYRKLGVGNRTAAVKKAAEL
                     GLIDWP"
     gene            3536..3745
                     /locus_tag="VHM69_12115"
     CDS             3536..3745
                     /locus_tag="VHM69_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741185.1"
                     /translation="MRESCFCGRIGEVEDREPVTTATGEPALRCPEEACGHTDGLGWL
                     PEETRRLVVEEAARRSAARRGPTAA"
     gene            3963..4448
                     /locus_tag="VHM69_12120"
     CDS             3963..4448
                     /locus_tag="VHM69_12120"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2741186.1"
                     /translation="MQTYVKFWRWIGHKRWFAVAMRYALSKVDREIYRLSGGRLALTG
                     PPVLPILLLTTTGRKTGKKRTVPLTYLRDAKKLVVTCENFGLERKAGWPKNLLANPEA
                     TVQIGRQTSRYLSRPASEGEISRYWPRLLEVWPALQTYMERGGERFVFVLEPATQRQD
                     A"
     gene            4797..5273
                     /locus_tag="VHM69_12125"
     CDS             4797..5273
                     /locus_tag="VHM69_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741187.1"
                     /translation="MLRRLRDPRRGPNAGPERPPPAKGRRRAGRVSGVGVRDRAGETR
                     EISARLVVAADGRDSTLVRPADATSKIRPNNRFLYFTHYRNLRLASGKRSQMWLLEPD
                     AAYTLPNDDGVTLLTCMPAREKLPSITISKAASHASSKARPKDRTQVSHVLHLAPL"
     gene            5296..5655
                     /locus_tag="VHM69_12130"
     CDS             5296..5655
                     /locus_tag="VHM69_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2741188.1"
                     /translation="MPWEVEHTDEFEEWWDSISEEEQENIALTIEKLEEQGPSLGRPL
                     ADTLENSRHANMKELRPPGSNIRVFFAFDPRRRAILLIGGDKTGRWRDFYDEMIRVAD
                     DLYDEYLEELREEGEIP"
     gene            5652..6044
                     /locus_tag="VHM69_12135"
     CDS             5652..6044
                     /locus_tag="VHM69_12135"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741189.1"
                     /translation="MSGRNSFSKLRAKIDADPERRARMDEKRKAYDVLLRLSELSELR
                     RDRGLTQAELAEALSVSQPAISKLEAAASRGSVSGSDMLLSTLAGYIEALGGRLELHA
                     TFPGHPEEEIAVPVGATSASEGTDDAQN"
     gene            complement(6059..7069)
                     /locus_tag="VHM69_12140"
     CDS             complement(6059..7069)
                     /locus_tag="VHM69_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2741190.1"
                     /translation="MWPRKNKGETRVALNKAKSGREGGFLADASTTTLGEYLDGWLED
                     TRGTVRQRSWERYEQIVRVHIKPTLGRAKLKTLNPAQVRALYREKLEGGSSPRTVQYV
                     HVTLHKALEQAQGDGLVARNAAKGIRAPRPRKKEIAPLTPDQARAFLEAARGDRFEAL
                     FVLALHCGLREGELLGLRWDDVDLEAGTLRVRRTLSETRDGPIFEPPKNGKGRNVPLT
                     GAAVGALRDHLARQMREIDEGYEDNGLVFASQTGNTMSASNLVNRHFKPLLKRAKLPR
                     IRPHDLRHTCATLLLIKGVHLKYVQELLGHANISITLDTYSHVIPGIGNQTVAAMEEI
                     FT"
     gene            complement(7129..7536)
                     /locus_tag="VHM69_12145"
     CDS             complement(7129..7536)
                     /locus_tag="VHM69_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2741191.1"
                     /translation="MYLLDTDILSNLMKRAPASALVARLARVPPEDQFTSSVTLGELL
                     YGAHRSSRTAALLERIEETLLPELPVLPFDAAAARRYGEIRAELERRGNPIGDADTRV
                     ASIALSRGLTVVTGNERHFRRVPGLEIENWLKD"
     gene            complement(7536..7844)
                     /locus_tag="VHM69_12150"
     CDS             complement(7536..7844)
                     /locus_tag="VHM69_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2741192.1"
                     /translation="MVKKVRVTEAKAHLSALMARAGYAGERYLIERRGKPLAALVGVE
                     DLERLEAEGGSPRSRPRGALALVGAWGEFVEDDEIDAMLGEIYSARERDTGRPVDLEA
                     "
     gene            complement(7888..8022)
                     /locus_tag="VHM69_12155"
     CDS             complement(7888..8022)
                     /locus_tag="VHM69_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741193.1"
                     /translation="MLGGELTVARDDRLPAEWWQVGEHRRHAKRAEYGASGTGAPFRS
                     "
     gene            complement(8099..8260)
                     /locus_tag="VHM69_12160"
     CDS             complement(8099..8260)
                     /locus_tag="VHM69_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741194.1"
                     /translation="MTFCGETVRLVRRARAQGAVSRMRKAAAREGADSMSREELEKAI
                     SAARSERAT"
     gene            complement(8486..>8707)
                     /locus_tag="VHM69_12165"
     CDS             complement(8486..>8707)
                     /locus_tag="VHM69_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEX2741195.1"
                     /translation="VTVVCALSSRAGYALFDGASVATVAFVQSFAGGALLTMLADTMM
                     PEAFKYGGKRVGLFTTLGFALAFLISTIE"
BASE COUNT         1536 a         2848 c         2812 g         1511 t
ORIGIN      
        1 agtagagcca gtcttctacc gcctccctgg tttcgtcatc ggcagagatt acttcgagct
       61 gggagagtgg aacggccaat tcttcctcat cccagtccat ctcgacgaac atatcgtgtt
      121 cgcaatcctc cacgggagcc atgccgatca cctcgacctc ttctcccacc ttcagcggag
      181 aggttgatct tctggcggcg caccgtgcaa ggaaggggaa gttgattttc atttcgaggt
      241 agtaatatca gcctatggcc cgttcttgtt cgttataggc atcgacgacg gcctccatgg
      301 agatccgatg ctctctggct tcgtccgttt cgatctcggg tcttccaaac cactcgacca
      361 ctgcaaacca ctccatcctt gctggcccgt caccccatac cataagtctt tgggcctgga
      421 tgcacaatct ccgaaatgtg ggcacatcag gcgttcgacg accgtggctg cgacctcggt
      481 gcgtaggctt gccaccttcc cactatcggc cgtcggagaa ccttatttca tattcgggcc
      541 acatactgca atgtggtgat catctgatca tcgcgcggac ttcctgcggc cgggatcgcg
      601 ggcagcgcga gtatggccgg ggccactacc ggcgtgccgt ccgcgccggg cgcgaggcgg
      661 cccctcgtcg gctcgagcgt cgcttcgggt tcgcgctcgg ccaccctacg ccctcacgct
      721 ctttccattc cgagaagaag gcgtctacat aacctacttc ggaggcgttt tcgggcgcca
      781 taggccgtgc caggggggcg ccgtcaccag acgccgctga aagtcggcac cgtgcgggcc
      841 gtttgggcgg gtcctacgag aatgccaaaa atcacccatc cgtacgatgc gctaagtccg
      901 tatccctgat accgtggtga caaggaggca ggagaacgtg cccacgggcc gcgcacagac
      961 cccgaatttc cccgggatcg ccgccgatcg gcgaggagac ggcaggccga ccacctgggc
     1021 gggagtggcg tcgaagggca acctgccgct ggaactctcc agcttcgtgg ggcgggagcg
     1081 tgaaatctcc caggtccgcg gcgtcatggg ggacgcccgg ctgctgacac tcaccggacc
     1141 cggagggtgc ggcaagacgc ggttggcgct gcgcgtggcc gcagacctgg cgggatccgg
     1201 ggagcgcttc ggggacgggg cctggtgggt agatctctcg tcgctctcgg atccgagcct
     1261 ggtcccgagc acggtggcct ccgctttcgg tgtgcgcgag gcgcctgacc ggacgccgac
     1321 cgaggcgctg gccgccttcc tcgaggcgaa gagtccgttg ctcgttctgg ataactgcga
     1381 gcacctcgtc gaagcgtgcg cgaccctggg cgacgtgctg ctgaggagct gtagcggctt
     1441 gaagatcatg gcgaccagcc gtgagcccct gggggtggcc ggcgagattt cctggcccgt
     1501 gcccccgctc gcgctgcccg accccaacag gcgactaaac gcgcaggatc tgctccgcta
     1561 ctcggcggta cgtctcttcg tcgagcgtgc tcgcgccgca tctttcggtt tctcctttac
     1621 ggaagagaac gcttcggtgg tggccgccct gtgcgcgagg ctcgacggga tgccgctcgc
     1681 tatagaactc gccgcctcga gggtaagggt gctctctctg cgccagatcc tggagaggct
     1741 cgacgaccgc ttccgtctct tgaggggcag caggacctcc gtcccgcgac acaagaccct
     1801 ggaggcgacg atcgactgga gccacgaact gctctccgag gaggagaaga tccttttccg
     1861 taggctctcg gtgttcgcgg gggggtggac gctcgcggcg gcagaggagg tctgcgcgga
     1921 cgacgggatc gcggaggacg agatcctgga actgctctcg tctctggtgg acaagtcgct
     1981 ggtgctcgcg tcgcaagtgg agggtagcgg tgaggcgcac taccggatgc tccagacgat
     2041 ccagcagtac gccgcgcaga ggccagggga accgggagat gcggaggccg tcggtcggcg
     2101 tcacgcggag tacttcctct ccctggcgga gagggccgag ccggcattgg ccggcccgga
     2161 gcaggcggct tggatggagc gtctggagga agagcacgac aacctcaggg cggctttggg
     2221 gtggttcgaa gagaagggag aagcggagcg gggcttgagg ctcgcggcgg cgtcgttgcg
     2281 attctggtgg cttcacggcc acctaagcga ggggagaacg tacctcgaga cgctccttga
     2341 ccgcccggga gcgcaggttc gggacgaggt gcgggccgag gcgcttcagg ttctcgggcg
     2401 catgatctac ctgcacgccg attacatcga cggcggttgg gaagtggcgc gctgccgact
     2461 ggaagagagc ctgcggatct accggcgcct gggaaacgag cggggcgtgg ccgacgtccg
     2521 gcagagcctc ggccgtgtct accctgaact cggcgagcgg agggcagcgt ttactttctt
     2581 ggaagagagc ctcgaaatag agcgacggtt ggagaacggt tccggcatcg cccgctcgct
     2641 cttcaacttg ggctacgcgc ggttgctcga tggcgaccta ccgtcggcgc gctcctactt
     2701 cgaggagggt ctgaggatct tccgaaagct ggacgacggg ttctggatca acgcctctct
     2761 ggtccacctg gggtttatag actgcgagga gggggagctc gccagggccc gcgccaagtt
     2821 tatacacatg aacgaggtgc tgcccctggt gcaattcccc tggggcacca cctacacgct
     2881 ggagggcttc gcgcgcctgg ccgcggcgga agggcagccg gtgcgcgctc tactcctggg
     2941 aggcgcgacg gccgagctgc gtcgcacctt cggcgtggcg atcggaccca ccaggcgggc
     3001 cggcttccgg cgcagcctga agccggcctg gcaagcgctg ggcgacgaag atggtaaggc
     3061 ggcttgggac aaaggccgga ccatgacgct agaagaggcc ctcgccctcg ccctggaaga
     3121 acccgagaag aagccggatc gcccccccgg aagcatcctc agcgcccgag aggtcgaggt
     3181 cctctcgctc gtcgccgagg ggctctccga cgccgaggtg gcaaagatgc tctacataag
     3241 cccccgcacc gtcggcaaca agctgcacgg agcgtaccgc aagctcgggg tggggaaccg
     3301 gaccgcggcc gtcaagaagg ccgccgagct gggcctgatc gactggccct gatccgaggc
     3361 cccgccgcac ccgtcagggc agtgcgtacc tgggccgacc gatcagcaca gagtagaaat
     3421 actccctttg cgcatccttg cccaaaggag taatcctgcc gatgcccgcc gccccagggc
     3481 aggataacgt cctctcagaa gcaaccggaa aagttccgaa ggaggttgaa gcgagatgcg
     3541 agagtcgtgt ttctgcggtc ggatcggcga ggtcgaggac cgagagccgg tgacgaccgc
     3601 cacgggagag ccagcgctca ggtgccccga ggaagcctgc ggccacaccg acggcctggg
     3661 gtggctgccc gaggagaccc ggaggctcgt cgtggaagag gcggcgcgga ggtcggcggc
     3721 acgccgcggc ccgacggccg cctgaggtcg agatctagcg ggcgaagcgc gcgtcattga
     3781 tgcgcgccac cacgaaagaa aggagcaaag ttgcacgcgg agtacatgaa ccactggagc
     3841 aaggaccgtc ccgagcagca aaagtcccgg agacggtagg tccgaactcg acgaagacga
     3901 cagtcaggag atggatcgtg tttacgcagc cagattcggg gacgagacta ggcgtagggc
     3961 ccatgcagac ctacgtgaaa ttctggcggt ggatcggcca caagcggtgg ttcgcggtcg
     4021 ccatgaggta tgccctctcg aaggtggacc gtgagatcta ccgtctcagt ggcggccgcc
     4081 tcgcgctgac tgggccgccg gtcctgccca tcctcctgct taccacgaca ggcaggaaga
     4141 cgggcaagaa gcgcaccgtc cccctcacct acctgcgcga cgcgaagaag ctggtcgtga
     4201 cctgcgagaa cttcggcctc gagcgcaagg ccggctggcc gaagaacctg ctggcaaacc
     4261 cggaggctac cgtccagatc gggcgtcaga catcccggta cctctcacgc cccgccagcg
     4321 aaggagagat ctcgcgctat tggcccaggc tcctcgaggt atggcccgcg ctccagacct
     4381 acatggagcg cggtggcgag cgttttgtgt tcgtactcga acccgctaca cagaggcagg
     4441 acgcatagga aagctccgta gcacttgagg tgagtagagg gacatgaccg agaaaacctt
     4501 cgacgcggct gtggtgggag cgagcattgc cggctgcgcg gcggcgacgc tcctggcgcg
     4561 ggagggggcg agcgtcgccc tgatcgaacg ccattccgac ccaaacgcct acaaggcgct
     4621 gtgcacccac ttcatccctc agctgtgccc accatcgaga ggctcggcct tttgcccccc
     4681 gtcgaagcgg ccggggcggt gccgaacgag tagacgtctt cacgcggtgg ggctgcattc
     4741 gcaacctcga aggtgtccct cacggctaca acatccgccg ccagaccctc gaccctatgc
     4801 ttcgccgcct cagagacccc cggcgtggac ctaatgccgg gccggagcgc ccaccgcctg
     4861 ctaagggaag gcgacgcgcg ggccgggtct ccggcgtcgg cgtcagggac cgcgcaggag
     4921 agacgcggga gatctccgcc cggctcgtgg tggctgccga cggccgcgac tccacgttgg
     4981 tacggcccgc ggacgctact tcgaagatta gaccaaacaa ccgtttcctc tacttcaccc
     5041 actaccgaaa cctacgcctc gcctccggca agaggagcca gatgtggctg ctggagccag
     5101 acgcagccta cacccttccc aacgacgacg gcgtgaccct cctgacctgc atgccggcca
     5161 gggagaagct gccttcgata acgatctcga aggcagcttc gcacgcttcc tcgaaggccc
     5221 gcccgaagga ccggacccag gtaagccacg tcttgcacct tgctccatta taacttgcag
     5281 gttatactaa cgacgatgcc gtgggaggtc gagcacaccg atgagttcga ggagtggtgg
     5341 gattcgatct ccgaggagga gcaagagaac atagccctca cgattgaaaa acttgaagag
     5401 cagggtccga gcctcggaag gccgctggcg gacacgctcg aaaactcacg gcacgcaaat
     5461 atgaaggagc taaggccgcc gggctcgaac atccgggtgt tcttcgcctt cgacccgcga
     5521 cggagggcga tactcctgat cgggggcgat aagacggggc gctggcggga cttctacgac
     5581 gagatgatcc gggtggccga cgacctctac gacgagtacc tggaggagct ccgggaggaa
     5641 ggagagatac cgtgagtggc agaaacagct tcagcaagct ccgcgcaaaa atcgacgcgg
     5701 acccggaacg ccgtgcacgg atggacgaga agaggaaggc ctacgatgtc ctactcaggc
     5761 tctccgagct ttcagaactg cgccgggatc gcggactgac ccaggccgag ctggccgagg
     5821 ctttaagcgt tagccagcct gcgatctcca agcttgaggc tgccgcctcc cggggaagcg
     5881 tctccggcag cgatatgctc ctctctacgc tcgccggata catcgaggcc ctcggcggcc
     5941 gcctggagct acacgccacc tttcccggcc accccgagga ggagatcgcc gtacccgtgg
     6001 gagcaacttc ggcttcagag ggtacggacg acgcccaaaa ctaggccaac tgcggggttc
     6061 aggtaaagat ctcctccata gcagccacgg tctgattacc aatgcccggt atgacgtggc
     6121 tgtaggtgtc gagggttatg gagatgttgg cgtgcccgag gagttcctgc acgtacttaa
     6181 gatgaacgcc ctttattagc agcagggtgg cgcaggtatg cctcaggtcg tgtggtctta
     6241 tgcgcgggag cttggcgcgc ttgagtagag gcttgaagtg gcgattgacc aggttggagg
     6301 cgctcatggt gttgcccgtc tgcgacgcga agacgagccc attatcctcg tacccctcgt
     6361 caatctcccg catctgtcgg gcgaggtgat ccctcagcgc cccgaccgcg gcgccggtga
     6421 ggggcacgtt gcggcccttg ccgttcttcg ggggctcgaa gatggggccg tccctggtct
     6481 ccgacagcgt gcgcctcacc cgcagcgtgc cggcctccag gtcgacgtcg tcccacctca
     6541 ggcccagcaa ctcgccctcc ctgaggccgc agtgcagggc caggacgaac aaagcctcga
     6601 acctgtcccc gcgcgccgcc tccaggaacg cgcgtgcctg atccggcgtc agcggcgcga
     6661 tctccttctt cctgggcctc ggggctctga tccccttggc ggcgttgcgg gccacgaggc
     6721 cgtcgccctg cgcctgctct agggccttgt gcagcgtcac gtgcacgtac tgcaccgtcc
     6781 gcggggacga acccccttcg agcttctcgc ggtagagggc gcgaacctgg gcggggttca
     6841 gggtcttgag tttcgctctg cccagcgtgg gctttatgtg gacgcgcact atctgctcgt
     6901 agcgctccca actgcgctgc cgcacggtgc cgcgcgtgtc ctcgagccag ccgtccaggt
     6961 actcgccgag ggtcgtggta ctggcgtcgg cgaggaagcc accctccctg cccgacttgg
     7021 ccttgttcag cgctacgcgc gtctcgccct tgttcttgcg cggccacacg tccccgtcgc
     7081 cgttgccccg tcccgcttcc tggccatgtt cgctcctcct tgcggcccct aatccttcag
     7141 ccagttctct atctctagcc ccggtacccg ccgaaaatga cgctcgttgc ctgtcactac
     7201 cgtcagtccc ctcgacaggg cgatggacgc aacgcgcgtg tcggcgtcac ctatcgggtt
     7261 gcccctccgc tcgagctccg cccttatctc cccgtagcgc cgggccgcgg cggcatcgaa
     7321 aggaaggacg ggcagctctg gcagcagcgt ttcctctata cgctccagca gagcggcggt
     7381 ccggctcgac cgatgcgcac cgtacaggag ttccccgagg gtcacgctcg aagtgaactg
     7441 atcctccgga ggcaccctgg ccaacctcgc caccagcgcg ctcgccggag cacgcttcat
     7501 gaggttgctc aggatgtcgg tgtccagcaa atacattagg cttccaggtc taccggacgc
     7561 ccggtgtcgc gctcccgcgc ggagtagatc tcccccagca tcgcgtcgat ctcgtcatcc
     7621 tccacgaact cgccccatgc ccctaccagg gccagcgccc cacggggtct cgaacgagga
     7681 gacccgcctt ctgcttccag tcgttccaga tcctcgacgc ccaccagcgc agcgagcggt
     7741 tttccgcgcc gctctatgag ataccgctcc ccggcgtagc cggcccgtgc catcagcgcc
     7801 gagagatgcg ccttcgcctc cgtcaccctc accttcttga ccatgacaac cccatttttg
     7861 ctgaacaggt cattatgacc tattatatca gcttctgaac ggggctcctg tccccgaggc
     7921 cccgtactcg gcccgctttg catggcgcct gtgctcgcct acctgccacc actcggccgg
     7981 gaggcggtcg tcgcgggcga cggtcagctc gccgccgagc accctgcctg tgccccaaag
     8041 ctgttcggct gcgcgggcac gaacgtgttg gtgtctggca ccaccctctc cagactcact
     8101 atgtggcgcg ctcgctccgg gctgcgctta tcgctttctc tagctcctcg cggctcatgc
     8161 tgtctgcgcc ttcgcgagca gcggcctttc gcatcctcga gacggcgccc tgcgcccttg
     8221 cgcgcctcac gaggcgaacc gtctcgccgc agaaggtcat ccgggaggtc gtcgatcaac
     8281 tgctggtaat gttcgcgccg atcttgctcg aacacggagt agatcttcgt aaattgcgcc
     8341 agcctccggc aagaccaact gcggcattac ttgggatggc accgggcgtg gcgtccggcc
     8401 actccagaca tgctgctccg gtcctcctta tgtgccctca tgcgcccgag tgtctagcgg
     8461 gcgcgtaccc gaaggcgtgg ccccttcact cgatcgtgct gatgaggaag gccaaagcga
     8521 aacccagggt ggtgaagagc ccgacccgct tgcccccgta cttgaaagcc tctggcatca
     8581 tcgtatcggc cagcatggtc aagagcgcgc cgccagcgaa ggactggacg aaggcgaccg
     8641 tcgccacaga agctccgtca aagagggcgt agccggcccg cgaagagagc gcgcacacca
     8701 ctgtgac
//
LOCUS       DATAIR010000508         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_61058, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000508 DATAIR010000000
VERSION     DATAIR010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61058"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..441
                     /gene="rpsR"
                     /locus_tag="VHM69_12170"
     CDS             190..441
                     /gene="rpsR"
                     /locus_tag="VHM69_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX2741196.1"
                     /translation="MGKRRTGPPRGGRPTKSKPCVFCKENVEYVDYKDYNVLRRFISD
                     RGKVRARRVTSLCPQHQREAASAIKRAREMALLPYVVGR"
     gene            447..896
                     /gene="rplI"
                     /locus_tag="VHM69_12175"
     CDS             447..896
                     /gene="rplI"
                     /locus_tag="VHM69_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX2741197.1"
                     /translation="MQVILNKDVEKIGQRGDIVDVSRGYVRNFLVPRGLTEVATPAKL
                     EEARRQMEETEERDRRLAERAEEIAGTLNKSVITIEARTGEDERLFGSVTAANIADAV
                     ERARGIRLDRRRIRLDEPIKALGTHQVPIQVHGEVEASVKIIVVPKL"
     gene            998..3649
                     /locus_tag="VHM69_12180"
     CDS             998..3649
                     /locus_tag="VHM69_12180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF005852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX2741198.1"
                     /translation="MERRGRIRSVETGADAARVPPHDLDAERAVIGAMLVSETAVAAV
                     AERLAAEDFYSEVHRIIYGAMMRLYSRGDPIDQLTLTNELRSVNEFERIGGRPYVFQI
                     VESVPTAANAGRYADIVRGKALLRAVIDVGSRITEDAFREPENVSEALDSAEQLIYGV
                     SNKTLREHLAPVSELAPGALEMIQRLYEQEGEVTGVETGFEDLDRLTTGFHKSDLVIL
                     AARPAMGKTAMALNAIWHAAGEKKMPVAIFSLEMSKEQLVQRLISQTTRIPAQALRSG
                     NVKAEDWPKLVRGVAEVSKAPIWIDDTAGVTLMEIRAKVRRLSSQLNAAGKMPLSLVV
                     VDYLQLMVGQGSRSENRQQEIAEISRGLKVLARDLDVPVLAIAQLSRAVEARHDKRPL
                     LSDLRDCVTGDTLVSLADGRRVPVRELVGTRPHVLSMSPEGKIVEAEAARVWSVGRRP
                     VFSVRLASGRVIRATARHRLFGADGWIQTGELRTGDRIALARSVPEPQDADKWPEARL
                     ALLGHLIGDGSYLSGQPMRYCTSSEENSLTVTKSAVEEFGVKVNRYAGRGNWHQLVLS
                     GNGNRWHPAGVNHWLRQLGIFGQRSHEKRVPESVFSLSNDQIAVLLKHLWATDGTIFA
                     RRTGTKGSSVICFSTNSRGLADDVAALLLRLRIVARIREVSQGSHRAMFTVSVSGTRD
                     QKLFLERVGAFGPRRSGAQRLAQSLETRQEATNVDTLPIEFFAKVKAAMAARGVSHRA
                     MAVARGTAYGGSSRFRFAPSRTLMVEYAGLLDDETLLAFAESDLFWDRVIEVVPCGEE
                     EVFDLTVPGPASWLADGIVSHNSGAIEQDADMVMFLYRDEYYNPDSDDKGIAEVIVGK
                     HRNGPTGKVQLAWMEQYTKFASLARRV"
BASE COUNT          777 a         1156 c         1215 g          626 t
ORIGIN      
        1 cgcacggacg gtttctacgt ggtcatggag ttcaccgcgg gcgagcgcac gctcgtcgag
       61 ctgaagcgca tcctgcgcgt ctccgacgac gtgctcaggc acatggtcgt gaagctcccg
      121 ccgagctacg cccacaagcc gctgcccgag cccgccgaag tcccggcttg acactactgg
      181 aggtaatgaa tgggtaagag aaggaccggg cctccccgcg ggggacgtcc cacgaagagc
      241 aagccttgcg tgttctgcaa ggagaacgtc gaatacgtcg actacaagga ctacaacgtg
      301 ctcaggcggt tcatctcgga ccgcggcaag gtccgtgccc gcagggtgac gagtctgtgc
      361 ccgcagcacc agcgtgaggc cgcctcggcg ataaagcgcg cccgtgagat ggcgcttctg
      421 ccatacgtgg tcgggaggta acagccgtgc aggtgatact caacaaggac gtcgagaaga
      481 tcggtcagcg cggcgacatc gtcgacgtga gccggggcta cgtgcgcaac ttcctcgtcc
      541 cgcgtggcct caccgaggtg gcgacccctg ccaagctaga ggaagcccgc cgccagatgg
      601 aggagaccga ggagagggac aggcgcctcg cggagagggc ggaggagatc gccggtaccc
      661 tcaacaagag cgttatcacc atcgaggctc gcaccggcga ggacgagagg ctcttcggtt
      721 cggtcaccgc cgccaacatc gccgacgccg tcgagagggc ccgaggcatc cgcctcgaca
      781 ggcgccgcat ccgcctggat gagcccataa aagccctcgg tacacaccag gtccccatac
      841 aggtccacgg tgaagtcgag gcgagcgtca agatcatcgt ggttcccaag ctgtagcaga
      901 taggtgtagc tgaactgtag cttcaggcac aatctaaacc agaaggtaaa ggctcccgtc
      961 ttgtaacggg ggcctttatg ctgcatcctg ataggtcatg gaacgccggg ggaggatccg
     1021 cagcgtagag accggggctg acgcggcccg tgtaccgccg cacgacctgg atgcggagcg
     1081 ggcagtcata ggcgcgatgc ttgtctcgga gaccgctgtc gcggctgttg cggagcggct
     1141 ggctgctgag gacttctact ccgaggtaca caggatcatc tacggtgcca tgatgcgact
     1201 gtactcgcgc ggcgacccta tagaccagct cacgctcacc aacgagctgc ggagcgtcaa
     1261 cgagttcgag aggatagggg gaaggcctta cgtcttccag atcgtcgaga gcgtcccgac
     1321 ggcggcgaac gctggcaggt acgcggacat cgtgcgtggc aaggcgttgc tgcgggccgt
     1381 catagacgtc gggagccgga tcacggagga cgccttcagg gagcccgaga acgtcagcga
     1441 ggcgctcgac tcggcggagc aactcatcta cggcgtctcg aacaagacgc tcagggaaca
     1501 ccttgcgccc gtctcagagc tcgcgccggg ggcgctcgaa atgatccaac gcctctacga
     1561 gcaggagggc gaagtaacgg gcgtcgagac cggcttcgag gacctcgacc ggctcaccac
     1621 aggcttccac aagagcgacc tggtcatact cgctgcgaga cccgctatgg gaaaaacagc
     1681 catggcgctc aacgctatct ggcacgccgc cggcgagaag aagatgcccg tggcgatctt
     1741 cagcctggag atgagcaagg agcaattagt tcagaggctc atctcgcaga cgacgcgcat
     1801 ccctgcgcaa gccctcagga gcggcaacgt caaggcagag gactggccga agctggtacg
     1861 cggggtagcg gaggtcagca aggccccgat ctggatcgac gacaccgccg gcgtgacgct
     1921 gatggagata cgggccaagg tcaggcgcct ctcaagccag ttgaacgccg ccggcaagat
     1981 gccgctctcg ctcgtcgtcg tcgactacct gcagctcatg gtgggccagg gcagccgctc
     2041 ggagaaccga cagcaggaga tcgccgagat cagccgcgga ctcaaagtcc tcgcccgcga
     2101 cctcgacgtc cccgtactcg caatagcgca gctctcccgc gccgtagaag cccgccacga
     2161 caagcgtcca ctcctgtcag accttcgaga ttgcgttacg ggcgatacgc tggtgtcgct
     2221 cgccgatggc agacgcgttc ccgtgcgaga gttggtcgga acgagaccgc atgtgctctc
     2281 catgtcgcca gaagggaaaa tagtcgaagc cgaagccgcc cgcgtgtggt cggtagggcg
     2341 gcgtccggta ttctcggtgc ggctcgccag cgggcgtgtg atccgggcta ccgccaggca
     2401 ccgactcttt ggcgcggacg gctggatcca gactggcgag ctacgtacag gtgacaggat
     2461 cgccctggca cgtagcgtac cggagccaca ggatgccgac aaatggccgg aggcccgtct
     2521 cgcgttgctc ggacacctga taggggatgg cagctacttg agtgggcagc ctatgcgcta
     2581 ctgtacctct tcggaagaaa atagcctgac cgtgaccaaa tcggccgtcg aggagttcgg
     2641 cgtaaaggtg aaccgctacg ctggaagggg caactggcac cagctcgtct tgagcggcaa
     2701 cgggaaccgc tggcatcctg caggggtaaa ccattggctg cggcagcttg gcatcttcgg
     2761 ccagagatcg cacgagaaac gggttcccga atccgttttc agcctcagca acgaccagat
     2821 cgccgtgctc ctcaagcacc tgtgggcgac cgacgggacg atcttcgccc ggcgtaccgg
     2881 gacgaagggt tcgtccgtga tctgcttctc caccaacagc aggggcctgg cggacgatgt
     2941 cgccgcccta ctcctgcgcc tccgtatcgt cgcccgcata cgcgaagtgt cccagggcag
     3001 ccatcgcgcg atgttcaccg tctccgtttc gggtacgcgg gatcagaagc tattcctgga
     3061 acgggttgga gccttcggtc cccgccgctc cggagcacag agactcgcgc aatctctaga
     3121 gacccggcaa gaggcgacca acgtagatac gcttccaatc gagttctttg cgaaggtcaa
     3181 ggcggccatg gcggcacgcg gcgtctccca ccgtgcaatg gcggtggcga gaggtacggc
     3241 ttacggcggc tcctcgcgtt ttcggttcgc cccttcgcgt acgcttatgg ttgagtacgc
     3301 cgggttgctc gacgacgaaa ctctgctcgc tttcgcggag agcgatctct tctgggaccg
     3361 cgtgatcgag gtggtcccat gcggagagga agaggtcttc gaccttaccg tgcccggccc
     3421 cgcgtcgtgg ctcgccgatg gcatcgtcag tcacaattct ggcgccatag aacaagacgc
     3481 agacatggtg atgttcctct accgggacga gtactacaac cctgactcgg acgacaaggg
     3541 catcgccgag gtaatcgtgg gcaagcacag aaacggtccc acaggcaagg tccagctcgc
     3601 ctggatggag cagtacacca agttcgcttc tctcgccagg agagtctaaa gaccagcctc
     3661 cagtagaagc gcaaattctc tgagaagaag tctgaaatct ttgtcccaaa gcctttacac
     3721 gccgaccggt cgatgatagt atcctctgtt acagagacgg cggaagaggt aaga
//
LOCUS       DATAIR010000509         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_448278, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000509 DATAIR010000000
VERSION     DATAIR010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448278"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VHM69_12185"
     CDS             <1..583
                     /locus_tag="VHM69_12185"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HEX2741199.1"
                     /translation="RLWGKDLSVPARSQMIYPNGGEVWCSPTSLSMVMAYWANKKGKR
                     SLNQKVPTVARGTYDYVYRGNGNWPFNTAYAGTNGLKASVNRFSSLGQVERWISKGVP
                     VVASISWGRGDLTGAPIPASSGHLLVIRGFTDAGNVIVNDPAASSNSGVRRAYQRDEF
                     YRAWFKSGSGGVAYIVRPEGWPVPDRTYSHGSW"
     gene            616..1992
                     /locus_tag="VHM69_12190"
     CDS             616..1992
                     /locus_tag="VHM69_12190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2741200.1"
                     /translation="MRTLRDIPKRLLGFKLLRRSASVLVLAFADALALALGLEGAAWI
                     SGGSEAALNLAPLLLAAWITLFTAFRLYDKAPVRRSPGGLVGAAFCWAGLVTIGAAIY
                     PQSGLGLGEIVSAVVLALLCAVALRFLYERAIGRIYRRGFGQTPVLLVGNEEDRTRLR
                     RMMERAPGAYVLAGEVDLGGADLTTLRERLDSTEARGVVLVGAERLPDDELLDLIHSV
                     RLRGVPLRVVPGAFALMRGRTILSEGMGLPLLEVRYPMLDNTQRTLKRVLDVAVSVGG
                     LALLSPLLLAVAVAVRLDSLGPVLFRQKRVGADEKVFVCYMFRSMQQDAELRQADLEE
                     LNEAEGPVFKMRDDPRVTRVGRFLRRWSIDELPQLMNVLKGEMSLVGPRPLPVRDFLR
                     MEESHKGRLGAVPGMTGYWQTSGRSDLSFEEMVRLDLYYIENWSLSFDLKIILKTLGA
                     VLRREGAY"
BASE COUNT          330 a          643 c          667 g          363 t
ORIGIN      
        1 gaggctgtgg ggcaaggact tatctgtgcc cgccaggtcg cagatgatct accccaacgg
       61 cggcgaggtg tggtgcagcc cgacgtccct ctccatggtg atggcctact gggccaacaa
      121 gaaaggcaag cggagcctca accagaaagt acccacagtg gcgcgcggca cctacgatta
      181 cgtctaccgc gggaacggca actggccctt caacacggcc tacgctggca ctaatggcct
      241 gaaggcctcc gtcaatcgct tctcctcgct cggacaggtg gagcgttgga tctcgaaggg
      301 cgtccccgtc gtcgcgagca tatcctgggg gcgcggagac cttacgggcg cgccaatccc
      361 cgccagctcg ggccacctgc tggtgatccg gggcttcaca gacgcaggga acgtgatcgt
      421 caacgacccc gcggccagca gcaactcggg cgtcaggcga gcctaccagc gagacgagtt
      481 ctacagggcg tggttcaagt ccggctcggg cggcgtggcg tatatcgtgc gtcccgaggg
      541 ctggccagtc cccgaccgca cctactccca cgggagctgg taggacggcg gtatacttgc
      601 cgcacggagg accccgtgcg gaccctgaga gacatcccca agaggctcct tggcttcaag
      661 ttgttgcggc gctctgcgag cgtcctcgtc ctcgcgttcg cggacgcgct agcgctggca
      721 ctggggctgg aaggggcagc ctggatctcg ggagggtctg aggcggccct gaatctcgcg
      781 ccgttgttgt tggcggcctg gatcacgctc ttcaccgctt tccgccttta cgacaaggcg
      841 cccgttcggc gcagtccagg ggggctcgtc ggggcagcgt tctgctgggc cgggctggtg
      901 acgatagggg ctgcgatcta cccccagagc ggcctcggtc tcggggagat agtgtcagcc
      961 gtcgtactcg ccctcctttg cgccgtcgcg ctcaggttcc tctacgagcg cgccattggc
     1021 aggatctacc gccgcggctt cggacagacg ccggtccttc tggttggaaa cgaggaggat
     1081 cggaccagat tgcgccggat gatggaacgc gccccaggcg catacgtact ggccggggag
     1141 gtggacctcg gaggggccga tctcacgacg ctgcgggaga ggctggacag cacggaggcc
     1201 cgtggcgtcg tcctcgtcgg ggccgagcgt ctgcccgacg acgagctact cgatctcatc
     1261 cactcggtgc gactgcgcgg ggtgccgctc agggtggtgc ccggcgcctt cgccctgatg
     1321 cggggccgta ccatcctctc ggaaggcatg ggattgccgc tcctcgaggt ccgctatccc
     1381 atgctggaca atacccagcg taccctcaag cgcgtcctcg acgttgctgt ctctgtgggc
     1441 gggctcgcgc tcctctctcc actcttactc gccgtggccg tcgcggtccg tctcgactcg
     1501 ctgggtcccg tgctcttcag gcagaagcgg gtcggggcag acgagaaggt cttcgtctgc
     1561 tacatgttcc gctctatgca acaggacgcc gaactgcgcc aggcggacct cgaggaactc
     1621 aacgaggccg agggacccgt cttcaagatg agggatgacc cccgcgtgac tagagtcggg
     1681 cgtttcctgc ggcgctggag tatagacgag ttgccccaac tcatgaacgt cctcaagggg
     1741 gagatgagcc tggttggtcc acggcctctc cccgtgcggg actttttgcg gatggaggag
     1801 tcacacaaag ggaggctcgg ggcggttccc gggatgaccg gatactggca gaccagcggc
     1861 cgcagcgacc tctccttcga ggagatggtg cgcctcgacc tctactacat agagaactgg
     1921 tcgctctcgt tcgacctcaa gatcatcctg aagaccctcg gcgccgttct acgacgcgaa
     1981 ggcgcgtact gatcagaccg tac
//
LOCUS       DATAIR010000510         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_61135, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000510 DATAIR010000000
VERSION     DATAIR010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61135"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VHM69_12195"
     CDS             complement(<1..622)
                     /locus_tag="VHM69_12195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2741201.1"
                     /translation="MPGSDRESLGRILLLAVLLALACAACDRTDEERGENGKDFEPIT
                     TGRAENADETTGPVATHVERVEVSTLVTNLDVPWSFAFLPDGDALVTERDSGRLLRVS
                     PSGEVREIQTLPESGSGEGGLLGVAVSLDYEEDHYVYAYYTTEDDNRVVRFRLGERPQ
                     PILTGIPANTFHDGGRIKFGPDGMLYVSTGDAGDSASSQDRNSLGGK"
     gene            complement(679..1524)
                     /locus_tag="VHM69_12200"
     CDS             complement(679..1524)
                     /locus_tag="VHM69_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2741202.1"
                     /translation="MNDNGAGTFDLGGDLPVKRLGFGAMRLCGPGIWGEPEDPGAAER
                     VLRRVVEVGINLIDTADAYGPEVNELQISSALHPYPDDLVIATKGGHVRPGPGRWETD
                     GSPQHLREACEGSLRRLRLDRIDLYQFHRPDPRVTFEDSVWALAELRDEGKVRHVGLS
                     NVGVREIVMALDIVPIVSVQNRYNLADRSSEAVIETCEREGIAFIPWHPLATGDLANP
                     GGPLDEVAARHEATAAQVAIAWLLTKSPMMLPIPGTSSVEHLEENVAAAELRLTDEEV
                     ATLSG"
     gene            1669..3012
                     /locus_tag="VHM69_12205"
     CDS             1669..3012
                     /locus_tag="VHM69_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741203.1"
                     /translation="MRPHVDRLRPLLGIVFLAGYVWYTFAEGFLGTALLLGVGCVLFV
                     ISVPWSSAFHRGFALVAFAALGAAILTGRFDPADFLAGMPTYFGIVAVLLVLSIAGYP
                     IRAARYEVQIRALMAALTQRGVGIKATAGVLGHVLGAVLDVGAFVLIDVISGRAAPKE
                     RLEALKWAGRAFSFAPLWTNLNVLTATTIVLTGVSYARLLAVTLPFALLGLAVTLLFA
                     QREGGEVEDVPQTPLDRGAAAVLLYPVLLVSAVALVNLTFPGLPLTAAIAVTVAIIVL
                     LIAALAAVLTRGPSPLARLGQETRGSLASSHAEFALFGSAGVLVLSLKALGALAPLGN
                     FLSALPIVLVAPALAAIIAVGFVAGIHVIPMVLLLNAAFPLDGGPAPALWAAALLLGA
                     QSTILLTPFSSAVTMLSRLTGLHPLEIGPRSNLGFGLIVAVAALVYLTLLTLLLL"
     gene            3081..>3738
                     /locus_tag="VHM69_12210"
     CDS             3081..>3738
                     /locus_tag="VHM69_12210"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2741204.1"
                     /translation="MVESRHRASGRSYANTTLVPDGSLRVTLLGRFEVSVDARIIRED
                     EWRLRKAASVVKLLALAPDHGLHREQVMHLLWPDLGARAATNNLHQALHVARRTIEPE
                     ATTHRFLPMRGEQLTLCQEGSAWVDVKAFERTVREARGSGRPAAYRAAIELYSGDLLP
                     LDRYEEWAERRREELRRTYLAILFELASVLEERGALDRAVETLQEIVAHEPVHEEAHV
                     G"
BASE COUNT          602 a         1223 c         1173 g          740 t
ORIGIN      
        1 tcttacctcc caaagagttc ctgtcctggg agcttgcaga atctccggcg tcgcccgtac
       61 tcacgtagag catcccgtcg ggtccgaact ttatcctgcc cccgtcgtga aaggtattgg
      121 cggggatgcc ggtcagtatc ggctgcggcc tctcgccgag ccggaagcgc accacgcggt
      181 tgtcgtcctc ggtcgtgtaa taggcgtaga cgtagtggtc ctcttcatag tcgagggaga
      241 cggcgacgcc gagtaacccg ccctcgcccg agccgctctc cggcagggtc tggatctctc
      301 gaacctcacc cgacggactt accctcagaa gccgtccaga gtcgcgctcg gtgacgagag
      361 catccccatc aggcagaaac gcgaaagacc agggcacgtc gaggttcgtg acgagtgtgc
      421 tcacctcgac ccgctccacg tgtgtcgcaa cgggacccgt ggtttcatcc gcattttcgg
      481 ctcttccggt agtgattggc tcgaagtctt taccgttctc ccctctctct tcgtcggttc
      541 tgtcgcacgc cgcgcacgcg agagcaagca ggacggcgag cagcagaatc ctgccaagtg
      601 actctctatc tgatcctggc atgctacgtc ctctccacgg cgacggagat tatattgcgc
      661 tccctcctgt ttcggcccct agcccgatag cgtcgcgacc tcctcgtcgg tgagtcttag
      721 ctccgctgcg gcgacgttct cttcgaggtg ctcgaccgaa gaggtgcctg ggataggcag
      781 catcatggga gatttcgtaa ggagccaggc gatggccacc tgcgccgcgg tagcctcgtg
      841 gcgggcggca acctcatcga gcggtccgcc tggattggcc aggtctcccg tcgccagcgg
      901 atgccagggg ataaacgcga tcccttcgcg ttcgcaggtc tcgatgacgg cttcggagga
      961 acggtcggcc aggttgtagc ggttctggac ggagacgata ggcacgatgt cgagcgccat
     1021 cactatttcc cttacgccca cgttggagag gccgacgtgc cggaccttac cctcgtcacg
     1081 tagctcggcc agggcccaga cggagtcctc gaaggtgacg cgggggtcgg gacggtgaaa
     1141 ctggtagagg tcgatcctgt cgagcctgag acggcgcagg ctcccttcgc aggcctcgcg
     1201 caggtgctgc gggctaccgt ccgtctccca gcgtcccggt cctggacgga cgtggccgcc
     1261 cttcgtggcg atcaccaggt cgtcggggta aggatgcaat gccgatgaga tctgaagctc
     1321 gttgacctcg ggaccgtagg cgtcggcggt gtctatgagg ttgatcccga cctcgaccac
     1381 cctgcgtagc accctctcgg cggcacctgg atcttcgggc tcaccccaga tccccggccc
     1441 gcacaggcgc atcgccccga acccgaggcg cttgaccggg agatctccac caaggtcaaa
     1501 ggtgcctgcc ccgttatcgt tcatccttta tcccttcctg tggctcgcac accagaggtt
     1561 accgcagcgc cggtatcgcg ttcatgatct agaatcgtgg ctgatgctga attccggcga
     1621 cctgaaagag ggcctgaggt aagagagaag tcacattggc tggagaacgt gagaccgcat
     1681 gtggacaggc tgcgtccatt gctcggcatc gtcttcctgg ccggttatgt ctggtacacg
     1741 ttcgcggaag ggttcctcgg gacggccctg ttattaggtg tggggtgcgt gctgttcgtg
     1801 atctccgtgc cctggtcgag cgcgttccac agagggttcg cccttgtcgc cttcgccgcg
     1861 ctaggggcgg cgatccttac ggggcggttc gaccccgcgg acttcctcgc aggtatgccg
     1921 acttacttcg ggatcgtggc ggtactgctc gtgttgagca tcgcggggta tccgatccgg
     1981 gccgcccgct acgaggtcca gatccgggcc ctaatggccg cgttgaccca gaggggcgtt
     2041 ggcataaagg cgacggcggg ggtcctcgga cacgtcctcg gcgccgtcct cgacgtgggg
     2101 gccttcgtcc tgatagacgt gatctcaggc cgcgccgccc cgaaagagcg cctggaagcc
     2161 ctgaaatggg cgggccgcgc cttctcgttc gcccctctct ggaccaacct gaacgtcctg
     2221 acggctacga ccatcgtgct taccggcgtc tcctacgcga gactgctcgc cgttacgctc
     2281 ccgttcgccc tgctcggtct cgccgtaact ttgctcttcg cccagaggga gggaggcgag
     2341 gtagaagacg ttccgcagac accgctcgac cggggggccg ctgccgtcct gctttatccc
     2401 gtgctgctcg tctctgccgt tgccctggtc aacctgacct tccccgggct cccgcttacg
     2461 gcggcgatcg ccgttacggt ggccatcata gtgctcctga tcgcagccct cgccgcggtc
     2521 cttacccgcg ggccgtcacc cctggcacgg ctcgggcagg agacgcgggg ctcgctcgct
     2581 tcctcgcacg ccgagttcgc cctcttcgga tccgccgggg tactcgtgct ctcgctgaaa
     2641 gccctcggag cgctcgcgcc gctcggcaac tttctctcag ccctgccgat agttcttgtc
     2701 gccccggcgc tcgcggcgat catcgccgtg gggttcgtag cgggcatcca cgtcatcccg
     2761 atggtactgt tgctgaacgc agcattcccg ctggacggcg gaccggctcc tgcgctgtgg
     2821 gcggcggccc tactgctcgg agcccagtcc accatcctgc tcaccccctt ctccagtgcc
     2881 gtcaccatgc tctctcggct gacgggcttg cacccgctgg agatcggacc gaggagcaac
     2941 ctggggttcg gcctcatagt ggccgtcgcc gcgttggtct acctgactct cctgaccttg
     3001 ctgctcttgt aaagtgagct ctaacccgcg acgcaataaa acccatcttg ctatcatggc
     3061 cctggagagg tgtcttgacg atggtggagt ctcgccatag agcatcggga aggtcctacg
     3121 cgaatacaac tctagtaccg gatggaagcc tgcgcgtaac actgctcgga cgtttcgagg
     3181 tctcggtaga tgctcggatt atccgcgagg atgaatggcg gctcaggaag gccgcgagcg
     3241 tggtaaagct gctcgcgctg gccccggatc acggcctcca ccgggaacag gtcatgcacc
     3301 tgctctggcc cgacctcgga gcgagggccg cgaccaataa cctccaccag gccctgcacg
     3361 tggcgagaag gaccatcgaa cctgaggcca ccacacaccg tttcctcccc atgcgcgggg
     3421 agcagctcac gctgtgccag gagggctctg cctgggtaga cgtaaaggcg ttcgaacgaa
     3481 ctgtacggga ggcgcgtggc tccgggcggc cagctgctta ccgtgccgcg atcgagctgt
     3541 actcggggga cctcctgccg cttgaccgct acgaggagtg ggcggaaaga cgcagagaag
     3601 agctacgaag gacttacctg gccatactct tcgaacttgc gtcggtcctg gaggagcgcg
     3661 gcgctctcga ccgggctgtc gagacgctcc aggaaatcgt cgcgcacgaa ccggtgcatg
     3721 aggaggcgca cgtaggac
//
LOCUS       DATAIR010000511         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_402602, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000511 DATAIR010000000
VERSION     DATAIR010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402602"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /gene="rplJ"
                     /locus_tag="VHM69_12215"
     CDS             complement(<1..354)
                     /gene="rplJ"
                     /locus_tag="VHM69_12215"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2741205.1"
                     /translation="MKREDKERVISDLAGKLRDGSAVLVDYQGINVAQSTDLRRESRE
                     NGVEFVVAKNTLTKRAADEAGIEGLEQFLVGPTALAFSEDPVASAKLMAKFADQVESF
                     VLKGGILEGDRVLDET"
     misc_feature    complement(365..497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(513..1202)
                     /gene="rplA"
                     /locus_tag="VHM69_12220"
     CDS             complement(513..1202)
                     /gene="rplA"
                     /locus_tag="VHM69_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2741206.1"
                     /translation="MGKRLLEQKTKIDRERLYGPREALEILKEVEGAKFDESVEAHVN
                     LNVDPRRADQMVRGTLMLPNGTGKTRRVAVFAVGEKAREAEEAGADVVGADDLAARIR
                     DENFMDFDVAVATPDQMSVVGRLGQVLGPRGLMPNPKSGTVTMDVGRAVEDIKKGKIE
                     YRVDRYGIIHSILGKKSFDLDSLVVNYFALREELQKVRPAPVKGRYFKSITLTTTMGP
                     SVKVDPSVEKE"
     gene            complement(1195..1632)
                     /gene="rplK"
                     /locus_tag="VHM69_12225"
     CDS             complement(1195..1632)
                     /gene="rplK"
                     /locus_tag="VHM69_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2741207.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNDATRDQMGQVVPVEITVYEDRSFTFITKTPPAAFLLKQAAGLDKGSGEPNRVKVG
                     AVSRAQVEEIARTKMPDLNAADVDGAAKIVEGTARSMGITVNG"
     gene            complement(1646..2176)
                     /gene="nusG"
                     /locus_tag="VHM69_12230"
     CDS             complement(1646..2176)
                     /gene="nusG"
                     /locus_tag="VHM69_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2741208.1"
                     /translation="MKKWYVVNTYSGHENKVRTTLERRIDSMGLDRHFGEIRIPTESV
                     IEIKDGKKVPTIQRQFPGYVLVNMDMNDSTWQLVRQTPGVTQIVGAGEKPLALSRAEV
                     ERLLHPGEVETREKVKTTVDYQIGETVKVIGGPLSDFTGSISDINVDQSRLKVLVSIF
                     GRETPVELSFNQVAKL"
     gene            complement(2196..2459)
                     /gene="secE"
                     /locus_tag="VHM69_12235"
     CDS             complement(2196..2459)
                     /gene="secE"
                     /locus_tag="VHM69_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2741209.1"
                     /translation="MGKKRGPATAKGSNKKSQQSQRQGGGVLARPRRFARDVRGELKR
                     VSWPDRDQLRQSTAVVLIIVLVLAAYVAALDVVFQSLVRLIFL"
     gene            complement(2490..2562)
                     /locus_tag="VHM69_12240"
     tRNA            complement(2490..2562)
                     /locus_tag="VHM69_12240"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2527..2529),aa:Trp,seq:cca)
     gene            complement(2576..2725)
                     /gene="rpmG"
                     /locus_tag="VHM69_12245"
     CDS             complement(2576..2725)
                     /gene="rpmG"
                     /locus_tag="VHM69_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2741210.1"
                     /translation="MRQLVTLACDECKRRNYHSRKNRRNTPDRIQLQKYCPWCRHHTA
                     HRETR"
     gene            complement(2782..>3169)
                     /gene="tuf"
                     /locus_tag="VHM69_12250"
     CDS             complement(2782..>3169)
                     /gene="tuf"
                     /locus_tag="VHM69_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX2741211.1"
                     /translation="DSAQAGDNIGALLRGVKRDDIERGQVLAKPGSITPHTRFKAEVY
                     VLSKEEGGRHTPFFSNYRPQFYFRTTDVTGEIRLEEGVEMVMPGDNTVMNVELISPIA
                     MDEGLNFAIREGGRTVGAGVVTEIVE"
BASE COUNT          532 a         1096 c          862 g          679 t
ORIGIN      
        1 ggtctcgtcg agcacccggt cgccttcgag aatgccgccc ttcaggacga acgactcgac
       61 ctgatccgcg aacttcgcca tcagcttcgc gctcgccacc gggtcctccg agaacgccag
      121 cgccgtcggc cccacgagga actgttccaa accctcgatc ccggcctcgt ccgccgcccg
      181 cttcgtcagc gtgttcttgg cgacgacgaa ctccacgccg ttctcccggc tctcccgtcg
      241 caggtcggtg ctctgcgcca cgttgatgcc ctggtaatcc acgagcaccg ccgaaccatc
      301 gcgcagcttc cccgcgagat ccgagatcac cctctccttg tcttccctct tcaactcaac
      361 tcctccgcca aatgtaaaac cccgaggccg cagaggcgct cggggctaaa gagatcccgt
      421 aaagctttcc gcaacctcgg ccggatgatt aagctctcgc gagccccggc tgtcttcggt
      481 ctactctatt aacttaaagt ccgcgcgaga tactactcct tctccaccga agggtcaacc
      541 ttgaccgacg gacccatcgt cgtcgtgagg gtgatcgact tgaagtagcg gcccttcacc
      601 ggcgccggac ggaccttctg aagctcctcg cgcagggcga aatagttgac cacaaggctg
      661 tccagatcga aggacttctt gccgaggatg gagtggatga tgccgtagcg gtccacacgg
      721 tactcgatct tgcccttctt gatgtcctcg accgcgcgtc cgacgtccat cgtaaccgtc
      781 ccgctcttcg ggttcggcat caggccgcgc ggaccgagca cctgcccgag ccgtcccacg
      841 acgctcatct gatccggcgt cgcgaccgca acgtcgaaat ccatgaagtt ctcgtcccgg
      901 atgcgggccg ccaggtcgtc agcgcccacc acgtccgcgc cggcctcttc ggcctcgcgg
      961 gccttctcac ccacggcgaa gacggccacc cggcgcgtct tgccggtgcc gttagggagc
     1021 atcagggtgc cgcggaccat ctggtcggcg cggcgcgggt ccacgttcag gttaacgtgc
     1081 gcctcgacgc tctcgtcgaa cttcgcgcct tcgacctcct tgagtatctc caacgcctcg
     1141 cgcgggccat acagccgctc gcggtcgatc ttcgtttttt gttccaaaag tcgcttaccc
     1201 attgaccgta atccccatgc tccttgccgt cccctcgacg atcttcgccg cgccatcgac
     1261 gtcggcggcg ttgaggtcag gcatctttgt gcgtgctatc tcctcgacct gggcccggct
     1321 cacggcgccg accttcacgc ggtttggctc gccgctgccc ttgtcgagtc cggcggcctg
     1381 cttgagcagg aaagccgcgg gcggcgtctt ggttatgaag gtgaacgagc ggtcctcgta
     1441 gaccgtgatc tccacgggga ctacctggcc catctggtcg cgcgtggcgt cgttgaactg
     1501 ccgcacgaac tcaccgatgt tcacctgcgc ctgtccgagg gcgggtccta ccggcggcgc
     1561 ggggttcgcc tgcccgcctg tgatctgcaa cttcaatttg cgcgctacgc ggcgcccgcg
     1621 tcctctaccc ataagaatcc tctcgttaga gcttggctac ctggttgaag gagagctcaa
     1681 cgggcgtctc gcgcccgaag atggagacga gcaccttcaa cctcgactgg tcaacgttga
     1741 tgtcggagat ggagcccgtg aagtcagaga gcgggccgcc gatgaccttg accgtctctc
     1801 cgatctggta gtccaccgtg gtcttgacct tctcacgcgt ctcgacctca ccgggatgca
     1861 agagcctctc gacctcggca cggctgagag ccagcggctt ctctcccgcg ccgacgatct
     1921 gggtgacccc gggcgtctgg cgcacgagtt gccaggtgct gtcgttcatg tccatgttca
     1981 caaggacgta gccagggaac tggcgctgta ttgtcgggac cttcttgccg tccttgatct
     2041 cgatgacgct ctcggtcggg atgcggatct caccgaagtg cctgtccaga cccatcgagt
     2101 cgatgcggcg ctccagcgtc gtgcgcacct tgttctcgtg ccccgagtat gtatttacga
     2161 cgtaccactt cttcaaaacg aaacgctcct aatgcctaca ggaagatcag gcgcaccagg
     2221 ctctggaaca cgacgtccaa agcggcaacg taagcagcca gtacgaggac gatgatgagc
     2281 acaacggccg tgctctgcct tagctggtcg cggtcgggcc aactgacccg cttgagctca
     2341 ccccgcacgt ccctggcgaa ccttctcgga cgggccagca cgccgcctcc ctgcctctgg
     2401 gactgctgcg acttcttgtt ggaccccttc gccgtcgcgg gcccgcgctt cttacccatt
     2461 gtgtcctcgc attgctttgc ctctcgaagc agggcaggag ggactcgaac ccccgacctg
     2521 cggttttgga gaccgctgct ctaccaactg agctactgcc ctctgtctac cgattctacc
     2581 gggtctccct gtgcgccgta tgatggcggc accacgggca atacttctgt agctggatgc
     2641 gatccggggt gttacgccgg ttcttcctgc tgtggtagtt ccggcgcttg cactcgtcgc
     2701 aagccagcgt tactaattgt cgcacaactt acctccaaat cacagggcgg cgtcttcgcc
     2761 gccccgagca ccgacggtgc cttattccac tatctcggtg acgacaccag caccaacagt
     2821 tcgtccaccc tcgcggatgg cgaagttcaa cccctcatcc atagctatcg gtgagatcaa
     2881 ctcaacattc attaccgtgt tgtcccctgg catcaccatc tccacgcctt cctcaagacg
     2941 tatctcccct gtcacatcag tcgtcctgaa gtagaactgc ggacggtagt tggagaaaaa
     3001 cggtgtgtgt ctacccccct cttccttact gaggacataa acttctgcct tgaagcgtgt
     3061 gtgtggggtt atcgatcctg gctttgccaa gacctgccct cgctctatgt catctcgctt
     3121 cactccacga agcaaggctc ctatgttgtc ccctgcctgc gcactgtcc
//
LOCUS       DATAIR010000512         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_403106, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000512 DATAIR010000000
VERSION     DATAIR010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403106"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..706)
                     /locus_tag="VHM69_12255"
     CDS             complement(329..706)
                     /locus_tag="VHM69_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741212.1"
                     /translation="MLNLGYPWWLVVSVLVVFALLTYYVLGGRVSEGLRVPLAVALAP
                     VCTAAAVAVAVVLTAFLSPRYETPIGPVEQPDRPPPATTPDTTRLETTRLETIGPAES
                     PAASPSASPSASPSASSTASPSP"
     gene            complement(1188..1400)
                     /locus_tag="VHM69_12260"
     CDS             complement(1188..1400)
                     /locus_tag="VHM69_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741213.1"
                     /translation="MGGRRRRLSREDLEAMRQEFIVGEREGDLDDDLHQIRRSARLGV
                     SPEDWARARGLAPAYARALRRYLDQK"
     gene            1625..>2836
                     /locus_tag="VHM69_12265"
     CDS             1625..>2836
                     /locus_tag="VHM69_12265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX2741214.1"
                     /translation="MLARMNPVKCGATVITLLILLVLAPGASLDRAVAQEKEPQAPPG
                     APRVNAGSWALIDTDTGMYLAGKAPDKRVPIASTTKIMVALVALDEGVNLDKEMTVSE
                     DAAAYAGSIYSNVGLYPYDRVSVRDLLTAALVPSGTDAVYALAENLGGGSVDEFVGTM
                     NDKAKELGLKNTHFENPAGLEDQDHYSSARDLAKMTRAAMKYPEFREIVSTPEATIST
                     QDRKIDVVNTNLLVVPNSGYDYGAATGIKTGTSPQAGPCLVASASNGDESYIAVVLDA
                     ASDLQRFDASRTALEYGFGEYEHEPLVERGDAFGEVSLPYRRDESVNLVAAKSVSALA
                     GPGLEVERSPTHEEAPPAAKSGRKLGTVEVTVEGRSVGNSPLVVQKGYEEASLWQKVT
                     YWAGGLKRWVLS"
BASE COUNT          576 a          872 c          924 g          464 t
ORIGIN      
        1 gcgccccggc aaccagaacc ttcatgcaga tacctccctg gatctcgaac cttccagaag
       61 tttatacccg ttacgggccg atccgagcct tcggacaggg ggccggtctg attccggggc
      121 cggcaggaaa ccggcgccca tttgctgtga tgaaagggaa agataggaga gtagcgggcg
      181 ccggttgcac cagactttag cccctgcatg gcggacgcgg gtaacagaga ggcaaacgtt
      241 cggtaacgga atcgcaacaa ccttcgagca gggacacctc tggtggcggt tgttcggaag
      301 gcgtccgatc aggccctacg cagcccgctc aaggagacgg agacgccgtc gaagaggcac
      361 tcggcgaagc actcggtgag gcactcggtg aagcggccgg tgactctgcc gggccgatgg
      421 tctcgaggcg cgtggtctcg agtcgcgtcg tatcgggggt ggtcgcaggc ggcggtcggt
      481 ccggctgctc tacaggaccg atcggtgttt catagcgtgg ggacagaaac gcggtaagca
      541 caacggcgac cgccaccgcg gcagcggtgc ataccggggc gagggcgacg gccagaggca
      601 cgcgcagccc ttcggagacc cgtccgccca gcacgtagta tgtgagcaga gcgaatacga
      661 cgagaacaga gaccacgagc caccaggggt atcccagatt cagcacaccc atgctcctaa
      721 ttgtcccagc gcctcgccgc gagtacggat cgaatccctg tgaacgccag ggcaacgacg
      781 cgggccagct ccacgcagag caacgccggc gtcaggtatc ctgtctgtgg agtgcttatc
      841 aggttcccct tcctcgggtg gcttacgttg cgtagtctaa aggatgaatg taacgtcgct
      901 gtaacgatcc aataacgatt gctgatcggc tctctggcgg ccctcgatat cagccgggag
      961 gcggagcgtt cgcaataggg taacgcgcac agagaaggat cgggccggta aggaggggga
     1021 aggtaccgaa ggcccgatcc tacgggtaat atggccggac ctcaggatcc tgggaggtgc
     1081 tcctggcccg gtcttgcgat actttacgat gcccacgaca gacggcgcat cccccaaatg
     1141 agtgattttt cgtgcgcgat cagaaaaaat cagggcgttc acggacctca tttctggtca
     1201 aggtacctcc gaagcgccct tgcgtacgcg ggcgccaggc cgcgcgccct ggcccaatcc
     1261 tcggggctca cgcccagcct ggcgctgcgg cgtatctgat ggaggtcgtc gtccaggtcg
     1321 ccttcgcgtt cgccgactat gaactcctgg cgcatggcct cgaggtcttc tctggacaac
     1381 ctcctgcgac ggcctcccat gcacctcctc tcgccgaaag agctgggacc accttaagcg
     1441 taaccccacc ccgccgcaac ggcaacggtt atcgaccggg tctttataac taacggtcaa
     1501 cggggtggta agggacggcc ctctcgtgca aaataccttc gtccgccgcc ggcggcttcg
     1561 ttgtaagggg agcgtggccg aagaccttca tccggccagt catcaaggca gaggagagca
     1621 acgggtgcta gcaagaatga accctgtgaa gtgcggcgcg actgtgatca cgctcctgat
     1681 cctgctggtc ctggcgcctg gagcgtcact tgacagggcc gtggcacagg agaaggaacc
     1741 tcaggcgcct ccgggagcac ccagggtgaa cgccggttca tgggcgctga tcgacaccga
     1801 cacgggcatg tacctggctg gcaaggctcc ggacaaacgg gttccgatag cctcgaccac
     1861 caagatcatg gttgccctcg tggcactcga cgaaggcgtg aacctcgaca aggagatgac
     1921 ggtctcggag gatgccgccg cgtacgcggg ctcgatctac agcaacgtgg gactctaccc
     1981 gtacgaccgc gtgagcgtca gggacctcct gacggcggca ctcgtgcctt ccggcaccga
     2041 cgcggtctac gccctggccg agaacctcgg gggcgggagc gtagacgagt tcgtcgggac
     2101 gatgaacgat aaggctaagg agttgggcct gaagaacacc cacttcgaga accccgccgg
     2161 cctcgaagac caggaccatt actcgagcgc ccgagacctg gcgaagatga ccagggcggc
     2221 catgaagtat cccgagttcc gcgagatcgt cagcacaccg gaggccacca tctcgacgca
     2281 agacaggaag atagacgtcg tcaacaccaa cctgctcgta gttcctaatt ccggctacga
     2341 ttacggggcg gcgacaggta tcaagactgg cacttcgccc caggcgggtc cctgcctcgt
     2401 ggcctcggcc agcaacggcg acgagtccta catcgcggtg gtgctcgacg ctgcgagtga
     2461 tctgcagcgc ttcgacgcat cgaggacggc gctcgagtac ggcttcggtg aatacgagca
     2521 cgaacctctg gtggagcgcg gagacgcctt cggtgaggtg tcactaccct accgcaggga
     2581 cgagtcggtg aacctcgtcg ccgcgaagag cgtatccgcg ctcgccggtc ccggtctcga
     2641 ggtcgagcgc agcccgacgc acgaggaggc tccccccgcc gcgaagtccg gacgcaagct
     2701 cggcaccgtc gaggtcacgg tggaaggccg cagcgtgggc aacagccccc tggtcgtcca
     2761 gaaaggctac gaggaggcct ccttgtggca gaaggtgacg tactgggcgg gaggactgaa
     2821 gaggtgggtc ctgtcc
//
LOCUS       DATAIR010000513         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_246314, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000513 DATAIR010000000
VERSION     DATAIR010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246314"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..761
                     /gene="nadD"
                     /locus_tag="VHM69_12270"
     CDS             117..761
                     /gene="nadD"
                     /locus_tag="VHM69_12270"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2741215.1"
                     /translation="MKIGIFGGTFDPVHQGHMIVAEQVMTELDLAHVVFVPGGIPPHK
                     EASSVRATAEDRLAMVEAAVEGNERFSVDRVEIDAGRPMHSVETVSILKERSPDDDWY
                     FITGADEVSNLLAWKEPDRLLEQVVMVAATRPGYDLSRLGHLEAALRNFDRIFPVECT
                     RVDISATGIRRRMLQKKSIRYLVPEGVREIIEDRRLYEGDGKRAEGGLLREEIR"
     gene            758..1156
                     /gene="rsfS"
                     /locus_tag="VHM69_12275"
     CDS             758..1156
                     /gene="rsfS"
                     /locus_tag="VHM69_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX2741216.1"
                     /translation="MRRDESVARQESFGTEGEALTYEMAATAARAASEMFARDVILVD
                     LKGLVSYADYFVIASAQTERQTRRVAQEVIERMIEKGYRPRTKRLDEGTAWISLDFLD
                     VVVHIFTDEARDYYRLESLWRSAPQEHWQE"
     gene            1211..1283
                     /locus_tag="VHM69_12280"
     tRNA            1211..1283
                     /locus_tag="VHM69_12280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Ala,seq:ggc)
     gene            complement(1474..1920)
                     /locus_tag="VHM69_12285"
     CDS             complement(1474..1920)
                     /locus_tag="VHM69_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741217.1"
                     /translation="MGSGIVGPAVAVDIGQPSRGNNVVGMVEEGRRVPVNCDHDEVAA
                     GRGVAECPAKCRRTVKLTRDAAEALRVHPTRQLEDIDGMGDYYRGLVFATMKGTLLNP
                     TNLRKRSFAQLLVRADLPNQIFHQLRHTAAAILLHKNVNPKIVSEM"
     gene            2292..2513
                     /locus_tag="VHM69_12290"
     CDS             2292..2513
                     /locus_tag="VHM69_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741218.1"
                     /translation="MSHIGARTVYRIVVRDELSERYAMAFEGMEMKVKNGQTILTGEI
                     IDQPHLHGILDRIGALGLKLVSVEDLSEG"
BASE COUNT          581 a          762 c          875 g          501 t
ORIGIN      
        1 cttccagcgc gagcttgaga agatcggggt tgttggtgcc ctgcggcggg ctggggccga
       61 gccgggcgac gaggtcagga ttggcgagac ggtgttcgac tttcagtgag gtggacttga
      121 agatagggat cttcggcggg accttcgacc ccgtgcatca ggggcacatg atcgtggctg
      181 aacaggtgat gacggagctt gacctggcgc acgtcgtgtt cgtcccaggt ggcatcccgc
      241 ctcacaaaga ggcttcgagc gtcagggcca ccgcggagga ccgtctcgcc atggtcgagg
      301 ccgcggtcga ggggaacgag cgtttcagcg tggacagggt cgagatcgat gcggggcgtc
      361 cgatgcacag cgtcgagacc gtgagcatac tcaaagaacg ctcgcccgat gatgactggt
      421 acttcatcac cggcgcggac gaggtgtcga acctgctcgc gtggaaggag ccagaccggt
      481 tattggagca ggtcgtcatg gtcgcggcca cgcggcctgg ctatgacctc tccaggctcg
      541 gacacctcga agcggccttg aggaacttcg ataggatctt cccggtcgag tgtacgcgcg
      601 tggatatttc ggcgaccggg atccgccgga ggatgttgca gaagaagagc atcaggtacc
      661 tggtgcccga gggagtgcgt gagatcatcg aggacaggag gttatatgaa ggcgatggaa
      721 agcgggcgga aggaggtctt ttgagggagg agatccggtg aggcgcgacg agtcggtcgc
      781 aaggcaggag agcttcggga ctgaaggcga ggcgctgacc tacgagatgg cggctactgc
      841 ggcgcgcgcc gcgagcgaga tgttcgccag agacgtgatc ttggtggacc tcaaggggct
      901 cgtttcctac gccgactact tcgtgatagc cagcgcccag accgagcgtc agacgcgccg
      961 cgtcgcccag gaagtcatag agaggatgat cgaaaaaggc taccgcccgc gcacaaagcg
     1021 tctggatgag gggacggcct ggatcagcct cgacttcctc gacgtggtcg tgcatatctt
     1081 cacggacgaa gcccgcgact actaccgcct cgagtcacta tggcgtagcg ccccgcagga
     1141 gcactggcag gagtaagcac gttgacaccc ccaggcatgc tgcatatact ctgacgggtc
     1201 ttcttctcaa ggggccgtag ctcagctggg agagcgccgc gctggcagcg cggaggtcag
     1261 gggttcgatc cccctcggct ccactctctg aagtggctta tctatcggaa atactgggga
     1321 aaaagccccc ggtacgaaac tcgaggtcta ttacaccaat taccgcacca aagcgccacg
     1381 caaacgcgtc ttccatagct gcaaaggcgc tatcttgcat gttcggcaag acgtgagagt
     1441 acttgtgcag actgatagct atgctcgcgt ggtctacatc tccgaaacta tcttcgggtt
     1501 cacgttcttg tggagtagga tcgcggcggc ggtatgccgt agctggtgaa atatctgatt
     1561 cggcagatcg gcccgaacga gcaattgagc aaaggaacgc ttgcggaggt tagtggggtt
     1621 gagaagcgtt cccttcatcg tggcgaacac aaggccccgg tagtagtctc ccatgccgtc
     1681 gatgtcctct agctggcgtg tcgggtgcac tctgagagct tcggcggcgt ccctggtgag
     1741 cttgaccgtc cgcctgcact tagcgggaca ctctgccacg ccacggccag cggcgacttc
     1801 gtcgtgatca cagttgaccg gaacgcgacg gccctcctcg accatcccaa cgacattatt
     1861 cccgcgagac ggttgcccga tgtccacagc aacggctgga ccgactatcc ccgaacccac
     1921 ctccacggcc actcgactgc cacggtggag cccacggacg aggggatgtc ctcagcccga
     1981 gccaaccacc gccaactgcg cgacggccac ttgatgtgct tcacctcctc ctcgtggagt
     2041 tatccaccac tcagatagca cacagcctct acctcgcccc gagcacgata aggaaccaga
     2101 tcaagtccat ctaccgcaag cactgagtat cctcgcgcaa gggagccgtg gtggaggcgc
     2161 ttgccagagg gctcatctag catcaacgac gaacctgtta atcccctagg ggaaatatgc
     2221 tggtgagtga tgacagaccg tcgtgccgtc ctctaagctc ggagaaagtt atggaaagcg
     2281 gtggcagaca ggtgagccac ataggtgcca ggacggtgta tcggatcgtg gttcgtgacg
     2341 agctcagtga gcgatacgct atggcattcg aagggatgga gatgaaggtc aagaacgggc
     2401 agaccattct tacgggggag atcatcgatc agcctcacct ccatggcatc ctcgaccgta
     2461 taggcgctct ggggctcaag ctggtgagcg ttgaggatct gtccgaaggc tgaggctatg
     2521 agccgccaaa ctcagagagt gggatcgtct taagtaccac tccaaccgaa agacgacgga
     2581 cgaaggagga accgtgatgt tcgggaagca gcatcagcag gtagcgcaaa cccgagaacc
     2641 cggacacgtc gaaggtccct acaagatcga tcgtgttgga ctgggcacct acggtgtgga
     2701 ctgcctccgg ttgcaggac
//
LOCUS       DATAIR010000514         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_311042, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000514 DATAIR010000000
VERSION     DATAIR010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311042"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHM69_12295"
     CDS             <1..265
                     /locus_tag="VHM69_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2741219.1"
                     /translation="IGLAPVVGLGLFAFWWAVVLLSRYTSLGAIAVMVVSPVVFWLSG
                     QPLPYVLYTIIGGALVLWRHRENARALLKGTERKVGQRTERGS"
     gene            266..>2285
                     /locus_tag="VHM69_12300"
     CDS             266..>2285
                     /locus_tag="VHM69_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741220.1"
                     /translation="MLREAYGGKNILLTGGTGFLGTALVEKILRSLPDLGRLYLIVRP
                     SRGKRAAERFEKDVLGAAAFRGLREKLGDDFDERVKEKVRVLEGDVHAPSLGLGEDDL
                     SELSREVDVVIHSAASVIFDAPLDAAVDSNVRGTLGLLKLARDWEKRPLFMHISTAYV
                     AGTSKEDAPEAPPSKVSPNGTALDVREEVSALEEVVEEVDIASQERSLLRRFETEARR
                     ELGMVGEEDEVAERVDQLRRAWMRERLVERGTRRARELGWNDVYTFTKSLAERMVVEE
                     RGETPLVILRPAIIESSHKEPYPGWIQGTRMADPIIMAFAKGILREFPGDPNSYVDIV
                     PVDHVVNAILAAGVRRPAEPEVFQVASGERNPIRYRDLYDIVRDFFLRNPLRDSSGRP
                     VKVPEWSFPGRRRVEGRLKMELASLKVAGILAARMPEGHMASDARQRIARAEKRARMS
                     LYYSRIYGAYANMTATFSTSRTKQLYEGLTPEDREEFPFDIAEIDWRAWMQGTHLPAL
                     TTRPSRKKRRKVTDEKPGEIAAIFDVDGTLVGSNVVSYFAWLRMRELPPALRPLWLAG
                     FLTKIPYYWGLDKLSRAHFNRAFYKNYAGWEPARARHLGQESFAGFTLDRIFPDALQQ
                     LRDHKAAGHRVVLLSGALDFLLEPMKDLVDDVLCATLAEENGAYTGELT"
BASE COUNT          425 a          725 c          760 g          375 t
ORIGIN      
        1 catcgggctc gctcctgtgg tcgggctcgg gctcttcgcg ttctggtggg ccgtcgtgct
       61 cctgagccgc tacacttcgc tcggcgcgat cgcggtgatg gtcgtcagcc ccgtcgtgtt
      121 ctggctctcc ggccagccgc taccatacgt gctctacacg ataataggag gggcgctcgt
      181 cctgtggcgg cacagggaga acgcgcgtgc ccttctcaag ggcacggaga ggaaagttgg
      241 acagcggacg gagaggggga gctagttgct ccgtgaggcg tatggcggga agaatatact
      301 gcttaccggg gggacgggct tcctcgggac ggcgctcgtc gagaagatcc tgcgctccct
      361 ccccgacctg gggcgtctct atctcatagt caggccctct cgcgggaaga gagcagcaga
      421 acgtttcgag aaggacgtgc tcggcgccgc agccttccgc ggcctgcgtg agaagcttgg
      481 cgacgacttc gacgagcgcg tgaaagagaa ggtcagggtt ctcgaaggcg acgtccatgc
      541 gccttcgctg gggctgggtg aagatgacct ctccgagctc tcgcgcgagg tcgacgtcgt
      601 gatccactcc gccgcatcgg tgatcttcga cgctccgctc gacgcggccg tggactcgaa
      661 cgtgagaggg accctggggc tcctgaagct cgcccgcgac tgggagaaga ggccgctctt
      721 catgcacata tcgacggctt acgtggcggg aaccagcaag gaggacgcgc ccgaggcacc
      781 gccttccaaa gtctctccga acgggacggc cctcgacgta cgcgaggagg tgagcgccct
      841 cgaggaggtc gtcgaggagg tggacatagc ttcgcaggag cggtcgctgc tcagacgctt
      901 cgagaccgag gcgcgtcggg aactcgggat ggtcggcgag gaggatgagg tcgccgagcg
      961 ggtggaccag ctaaggcggg cctggatgcg cgagcgcctc gtcgagcgtg ggacgcggcg
     1021 ggcgcgggag ctcggatgga acgacgtcta caccttcacc aaatcgctcg cggagcggat
     1081 ggtggtcgaa gagagggggg agaccccgct cgtcatcctc aggccggcca ttatagagag
     1141 cagccacaag gagccttatc ctggttggat ccagggcaca aggatggccg accccataat
     1201 catggctttc gcgaagggca tcctgcgcga gttccctggc gacccgaaca gctacgtcga
     1261 tatcgtcccc gtcgaccacg tcgtgaacgc catcctcgcc gccggcgtcc gccgccccgc
     1321 agaacccgag gtcttccagg tcgcctcggg agaacgcaac ccgatccgct accgcgacct
     1381 ctacgacatc gtccgcgact tcttcctgag gaacccgctg cgcgactcca gcgggcgtcc
     1441 ggtgaaggtg ccggagtggt ctttcccagg ccgcagacgg gtcgagggac gcctcaaaat
     1501 ggagctcgcg agcctcaagg tggccggcat actcgcggca aggatgcccg aggggcacat
     1561 ggcctcagac gcccgccaga ggatcgcacg cgccgagaag cgcgccagga tgagcctcta
     1621 ctatagccgt atctacgggg catacgcgaa catgacggcg acattctcga cctcccgtac
     1681 aaagcagctc tatgagggtc tcacgcctga agaccgcgag gagtttcctt tcgacatagc
     1741 ggagatagac tggcgggcct ggatgcaggg gacacacctg cccgcgctca ccaccaggcc
     1801 gagccgcaag aagcgaagga aggtgacgga cgaaaaaccg ggggagatcg cagcgatctt
     1861 cgacgttgac gggactctcg taggttccaa cgtcgtctcg tacttcgcgt ggctcaggat
     1921 gcgggaactg ccgccggccc tgcgcccgct ctggctcgcg gggttcctca ccaagatacc
     1981 gtattactgg gggcttgata agctcagccg cgcccacttc aaccgtgcct tctacaagaa
     2041 ctacgcgggc tgggaacccg cgcgagcccg tcacctcggg caggagagct tcgccggctt
     2101 tacactcgac cgcatctttc ccgacgccct gcagcagcta cgggaccaca aggccgcagg
     2161 acaccgcgtc gtcctgctct cgggagccct cgactttctc ctcgaaccga tgaaggacct
     2221 tgtagacgac gtgctatgcg ccacgctcgc cgaagagaac ggcgcctaca ccggcgagct
     2281 cacgg
//
LOCUS       DATAIR010000515         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_33925, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000515 DATAIR010000000
VERSION     DATAIR010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33925"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VHM69_12305"
     CDS             complement(<1..564)
                     /locus_tag="VHM69_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2741221.1"
                     /translation="MTVGALFGQAAWGYDRARRQLVPGLDRFYGAVLESVPFGSEEEI
                     RVLDLGAGTGLLSAKVAERFPRSRVTLVDLSVEMLRVARRRFNNERGRFEFRTMDYAR
                     KPLPRIVGGYDLVVTALSVHHLTHGDKKELFEKVHHSLAGGGHFVNADQVSGETPEEE
                     TFYKEWWLRRVREAGVSEEDLATALSRM"
     gene            complement(561..1337)
                     /locus_tag="VHM69_12310"
     CDS             complement(561..1337)
                     /locus_tag="VHM69_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2741222.1"
                     /translation="MGREWDAGEYETLSAPQTRWGAEVLGRLDARGDEAAIDGGCGTG
                     RVTELLLAKLPEGSVLAVDISLAMVEAARRRFADAPRVRVERQDLLELQVTERVDLIF
                     STATFHWIKDHDRLFERLARALKPGGRLVAQCGGEGNISRATKATKETMEEEIFMQYF
                     VGWDDDKNYADAETTARRLEAAGFEEIETWMHDETAAFDSVDELARFLGTVVLGGHLE
                     RLPEEERGPFAAAVAEKIVAVDGSPALDYVRMNIMARKSA"
     gene            1389..2105
                     /locus_tag="VHM69_12315"
     CDS             1389..2105
                     /locus_tag="VHM69_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2741223.1"
                     /translation="MDSIEVISSGSKSGVGVLDKSVAILAFLSGRGSATLAEVVDGTG
                     LPRPTAHRLISALEAHHLVSRQEGRYVLGLRLLGWGNRAAGVGLVERARPVLEELRDE
                     SEESTQLYVREGDHRVCVASVERVTGLRDTVPVGAVMPLSRGSAGKIFLAFAPGGVDD
                     RPEAAELRGIRVRGWAESVAEREAGVASVSAPVFGEDGRLKAAVSASGPISRLGERPG
                     ERLSHLVVDAAREIEKALTL"
     gene            2426..2818
                     /locus_tag="VHM69_12320"
     CDS             2426..2818
                     /locus_tag="VHM69_12320"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX2741224.1"
                     /translation="MTRTFAIVIGLLFLVMGILGFVPGVTMDSEYLFGIFAVDAVHNL
                     VYMLVGALGVAAYYWDRSKLYCQVLGVFMLLIGIMGFIPALVFDEKLLGIIAVNLANN
                     ILHLVVGTVAAYLGFAPQVTGRRISPTH"
     gene            complement(2868..>3383)
                     /locus_tag="VHM69_12325"
     CDS             complement(2868..>3383)
                     /locus_tag="VHM69_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2741225.1"
                     /translation="TWGERAARWAEGLRRSQDLDHWASFHDSFVAMVDLLGRVARGES
                     PPSTVLILSGDVHHGYLTEATFTDGARSRFYQAVSSPLRNALPNKKSLLQSYAWSKPA
                     TFITRLLARLAGVAAEPLTWRMTHDAAFFDNQIATLELEDRSATITFEKAVLDASKEP
                     ALEKLYERRLA"
BASE COUNT          612 a         1006 c         1080 g          685 t
ORIGIN      
        1 catacgtgat agcgcggtgg cgagatcctc ctcggagaca ccggcctcac gcacccgcct
       61 cagccaccac tccttgtaga acgtctcctc ctcaggcgtc tcacctgata cctgatccgc
      121 gttgacgaaa tggccgccgc cagcgagtga gtggtgaact ttttcaaata gctccttctt
      181 gtcaccatga gtgagatgat gaacggagag tgcagtcacg acgaggtcgt aacccccgac
      241 tattcggggc aacggcttgc gcgcatagtc catggtccga aactcgaaac gcccccgctc
      301 attattgaag cgtcggcgcg ccaccctgag catctccacc gagaggtcaa cgagcgtcac
      361 gcgcgaacgc gggaacctct ctgcaacctt cgcgctcagt agacctgttc cggcgccgag
      421 gtcgagcacc ctgatctcct cctcacttcc gaatggcacg ctctccagca cggccccgta
      481 gaagcggtcg aggccgggca cgagttgcct gcgcgcccgg tcatagcccc acgcagcctg
      541 cccgaacagt gctccgaccg tcacgccgac ttcctggcca tgatgttcat ccggacgtaa
      601 tccagcgcgg gcgatccatc cactgcaact atcttctcgg ccaccgcagc ggcgaaagga
      661 ccgcgctcct cctcaggcag tctctccaga tgcccgccga gcactaccgt accgaggaag
      721 cgggcaagct cgtccaccga atcgaaggcc gcggtctcgt cgtgcatcca ggtctcgatt
      781 tcctcgaagc ctgcggcctc gaggcgtctc gcggtcgtct ccgcgtcggc gtagttcttg
      841 tcgtcatccc agccaacgaa atactgcatg aagatctctt cttccatcgt ctccttcgtc
      901 gccttcgtgg cgcgggagat gttgccctct cccccgcact gggcgacgag gcgtcctccc
      961 ggcttcaaag cccgcgccag cctctcgaag agccggtcgt ggtcctttat ccagtggaac
     1021 gtggcggtag agaagatcaa atccacacgc tcagtaacct gaagctccaa gagatcctgg
     1081 cgctccaccc gcacccgcgg cgcatccgcg aaccgcctcc tcgccgcctc gaccatagcc
     1141 agggaaatat cgacagccag caccgaaccc tcgggcaact tcgcgagcaa gagctccgtc
     1201 acccggcccg tcccgcaacc tccgtcgatg gcggcctcgt ctcctctcgc gtccaggcgc
     1261 ccgaggacct cagcccccca acgggtctgc ggcgcggaaa gcgtctcata ctcccccgcg
     1321 tcccactcgc gccccaagac cccacctttc ttgctttgtc tcatatattg agacataatg
     1381 accgacatat ggacagtata gaagttatct catccgggag caagagtggg gtcggggtat
     1441 tggacaagtc ggtggcgatc ctggcttttc tttcggggcg tggttctgcg acgctggcgg
     1501 aggtggttga tgggacgggg ttacctcgtc cgacggctca ccggcttata tcggcgcttg
     1561 aggcgcacca cctggtatcg cggcaggaag ggcgatacgt actgggattg aggctgctcg
     1621 gatgggggaa cagggctgcg ggggttggtc tcgtggagcg ggcgcggccc gttttggaag
     1681 agttgaggga cgagagtgaa gagagcacgc agctatatgt gagggagggg gatcacaggg
     1741 tgtgcgtggc gtccgtggag cgggtgacag gtttgaggga cacggtgccc gtgggtgccg
     1801 tcatgccgct ctcgcggggg tcggcgggga agatattttt ggccttcgcg cccggcggcg
     1861 tcgatgacag gcccgaggcc gcggagctgc gtgggatccg ggtccggggc tgggccgaga
     1921 gcgtggccga aagggaggcc ggggttgcga gcgtcagcgc gccggtcttc ggggaggacg
     1981 ggaggctaaa ggccgcggtg agcgcgagcg gcccgatctc acgcctcgga gaacggccag
     2041 gcgaacgcct ttcgcacctt gtcgtggatg ccgcacggga gatagagaag gccctgaccc
     2101 tctaggtttt cgatctcaaa gagattcttt acaacaaata atttttgtaa caacattcat
     2161 aacatatcgt tcaccaatct tcttcagaga agatatatct ttgggaaaca ggcagggatg
     2221 cggaccatga agcgcgcaag agttctgttt acgggtggaa tccggttatg accggcgtaa
     2281 gccggaggga ccgatgcggt gtgcggcttc gttctgcatg gtctccggga ggcgctgtgt
     2341 cttcggagga ggtatgtagc gaggtcgcat agataggtgg ttcgggtttt tcagtattcg
     2401 cagtcgagtc ttcaaaggag gtccaatgac tcgcactttc gcgatcgtga tcggtctgct
     2461 gttcctggtg atgggcatac tcgggttcgt acccggtgta accatggaca gcgaatacct
     2521 gttcggcatc ttcgccgtcg acgcggtaca taacctggtc tatatgctag tcggtgcgct
     2581 gggcgtcgcc gcctattatt gggatcggtc gaagctgtat tgccaggtgc ttggcgtttt
     2641 catgctcctg ataggcatca tggggttcat ccccgccctc gtcttcgatg agaagctgct
     2701 tggcatcatc gccgttaacc tggcgaacaa tatcttgcat ctcgtcgttg gcactgtcgc
     2761 agcctacctt ggcttcgccc ctcaggtcac tggacgccgc atctctccga ctcactaggg
     2821 gaccggccct agacgctgga gccggggtgt agatgccccg gctctatcta tgccaggcgg
     2881 cgttcgtaca gcttctccag ggcgggttct ttcgaagcgt cgaggacggc cttctcgaag
     2941 gtgatggtgg cgctcctgtc ttcgagctca agggtggcga tctggttgtc gaagaaggcg
     3001 gcatcgtggg tcatacgcca ggtgaggggt tccgcagcga cgcctgccag gcgggcgaga
     3061 agacgggtga tgaaggtggc gggtttcgac caggcgtagc tctggaggag cgacttctta
     3121 ttcgggaggg cgttgcgcag gggggagctg acggcctggt aaaagcggct cctcgcgccg
     3181 tcagtgaaag tagcttcggt gaggtagccg tgatggacgt cgccggagag gatcaggacg
     3241 gtggaagggg ggctctctcc gcgggcgacg cgtcctagca agtccaccat cgcgacgaat
     3301 gagtcgtgga aggaggccca gtgatcgagg tcctgcgagc gacggagtcc ttctgcccag
     3361 cgtgcggcgc gttcacccca ggt
//
LOCUS       DATAIR010000516         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_403666, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000516 DATAIR010000000
VERSION     DATAIR010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403666"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="ribH"
                     /locus_tag="VHM69_12330"
     CDS             complement(<1..445)
                     /gene="ribH"
                     /locus_tag="VHM69_12330"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX2741226.1"
                     /translation="MEERANHIEGNISAEGRSFGIVASRFNDFIVKALVDGALDGIRR
                     HGGEASAVDVAWVPGSYEIPVVAREMALSGRYDAILCLGAVIRGATAHFDYVAGGVAS
                     GISSVALETGVPVIFGVITTETIEQGIERAGTKMGNKGFEAAVSAI"
     gene            complement(454..1656)
                     /locus_tag="VHM69_12335"
     CDS             complement(454..1656)
                     /locus_tag="VHM69_12335"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX2741227.1"
                     /translation="MPFSPIEEVIEDIKQGKMVIVCDDEDRENEGDLTMAAELVTAED
                     INFMASHGRGLICLPMAGEIIDRLDIPEMVTHNASRMGTAFTASIEAKEGITTGISAA
                     DRAHTCRVAVDEATGPEDLVMPGHVFPLRAKPGGVLQRAGQTEAAVDLARLAGRTPAG
                     VICEIMKEDGTMARVPDLEKFSKEHGVKLVTVAQIIEYRHAYETQVKCAVETKLPTPF
                     GEFRLRAYENEIDDLTHVALVMGEPEGKDDVLVRVHSACLTGDALHSLRCDCGQQLEA
                     AMEHIADEGEGVIAYMQQEGRGIGLLNKMKAYHLQDEGMDTVEANQKLGLAPDLRDYG
                     IGAQILKDLDLKRIRLLTNNLTKVVGLRGFGLEITERVPIEMEPNGHNERYLKTKREK
                     LNHVFEKF"
     gene            complement(1646..2281)
                     /locus_tag="VHM69_12340"
     CDS             complement(1646..2281)
                     /locus_tag="VHM69_12340"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX2741228.1"
                     /translation="MFTGLVEEVGRVSALEEREMLRLTISASRVTGDTREGDSISVNG
                     ACLTVGEADELTLTFYAMPETLRRTSLGGLAEGSPVNLERAMAAGSRFGGHFVQGHVD
                     GVGEVLGVRPEGDAEVWEFGAPDAVLRYCVEKGSVCVDGISLTIVSVGEGTFTVSILP
                     QTRANTNLGELRVGDRVNLEADVIGKYVERLLEPRIVERFSNFERSVGDAV"
     gene            complement(2262..>2938)
                     /locus_tag="VHM69_12345"
     CDS             complement(2262..>2938)
                     /locus_tag="VHM69_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX2741229.1"
                     /translation="KLATTLDGRIAASGGDSRWVTGEDARRRAHELRAEAGAVLVGAN
                     TVRADDPMLTARDLPVRPPRITRVVLDPHLKTDPESKLARSADEAPVVVFAAEPTSDE
                     RRRSLKECGVEVVETPRSEEGLDLPFVLDELGERGIRGLLVEGGGETATRFVRKGLAD
                     KLTLFYAPKLLGSEGIPMIGVLRATKMAESLQFSVSEVEKIGEDVAVTLYPGAGGEEE
                     RVHRAC"
BASE COUNT          489 a         1028 c          852 g          569 t
ORIGIN      
        1 ctattgccga gaccgcagct tcgaaaccct tgttgcccat cttcgtgcct gcgcgttcta
       61 tcccctgctc gatcgtctcc gtcgtgatga cgccgaagat cacgggcacc ccggtctcga
      121 gcgccacgga cgagatgccg ctcgcgaccc cgccagcgac gtagtcgaag tgggccgtgg
      181 cgccccggat caccgccccg agacagagga tcgcgtcgta acgtcccgag agcgccatct
      241 ccctggcgac cacaggtatc tcataggaac caggtaccca ggcgacgtca acggctgaag
      301 cctcgccacc gtggcgcctg ataccatcga gcgccccgtc gaccagcgcc ttcacgatga
      361 agtcgttgaa gcgagaggcg actatcccga aggacctccc ctccgctgag atgttcccct
      421 cgatgtggtt ggctcgttcc tccacatgtc cccctagaac ttctcgaaga cgtggttgag
      481 cttctcgcgc ttcgtcttca ggtagcgctc gttgtggccg ttcggctcca tctcgatcgg
      541 gacgcgctcg gtgatctcaa ggccgaaccc acgcaggccc acgaccttcg tcaagttgtt
      601 ggtcaacaga cggatgcgct tgaggtccaa gtctttcagg atctgggccc caatcccgta
      661 atccctcaag tcaggcgcca gcccgagctt ctggttggcc tcgacggtat ccatcccctc
      721 gtcctgaagg tgataagcct tcatcttgtt gaggagcccg atgccccttc cctcctgctg
      781 catgtacgcg atgacgccct cgccttcgtc cgcgatgtgc tccatggccg cctcgagttg
      841 ctggccgcag tcgcaacgca gggagtggag cgcgtctccc gtcaggcatg ctgagtggac
      901 ccgcacgagc acgtcgtcct tgccctcggg ttcgcccatg acgagggcga cgtgggtgag
      961 gtcgtctatc tcgttctcgt aggcgcgtag acggaactcc ccgaatggag tcggcagctt
     1021 cgtctcgacg gcgcacttca cctgcgtctc gtaggcgtga cggtactcga tgatctgggc
     1081 gaccgtaacc agcttgacgc catgctcctt cgagaacttc tcgagatcgg gcacacgcgc
     1141 catcgtgccg tcctctttca tgatctcgca gatcacaccc gcgggcgtgc gcccggcgag
     1201 cctggccaga tcgacggcgg cctctgtctg ccccgcgcgt tgcagaacgc cgcccggttt
     1261 ggcgcgcaag gggaatacgt gtccaggcat gaccaggtcc tcgggaccgg tcgcctcgtc
     1321 aacggcgacg cggcaggtgt gtgccctgtc agcggcggag atgcccgtgg tgatgccttc
     1381 cttcgcctcg atggaggccg tgaacgccgt gcccatcctg gaagcgttgt gggtcaccat
     1441 ctcgggtatg tcgaggcggt cgatgatctc gcctgccatc ggaaggcaga tcagaccgcg
     1501 cccgtgggat gccatgaagt tgatgtcctc tgcggtcacg agctccgcgg ccatcgtcag
     1561 gtcgccctcg ttctcacggt cctcatcgtc acagacgatg accatcttgc cctgctttat
     1621 gtcctcgatc acttcttcaa tgggactaaa cggcatctcc gacactcctc tcaaagtttg
     1681 aaaatctctc tactattcgc ggctcgagca gccgctccac gtacttgcct attacgtcgg
     1741 cctcgaggtt caccctgtcc cccacgcgca gctctcccag gttcgtgttg gcgcgggtct
     1801 ggggcagtat cgagacggtg aatgtaccct ccccgaccga gactatggtc aggctgatgc
     1861 cgtccacgca gacgctgcct ttctcgacgc aatagcggag cacggcatct ggcgccccga
     1921 actcccagac ctccgcatca ccttcagggc gcacaccgag cacctccccg acgccgtcga
     1981 cgtgcccctg cacgaagtgt ccgccgaacc tgcttcccgc ggccatcgcc cgctccaggt
     2041 ttacggggct accctcggcg agtccgccga gcgacgtgcg cctcaaggtc tcgggcatgg
     2101 cgtagaaggt gagggtcagt tcatcagcct caccgaccgt caggcacgcc ccgttcaccg
     2161 agatactgtc gccttcgcgc gtatctccgg tgacccggct ggctgagata gtgagacgca
     2221 acatctcgcg ttcttccaga gccgagacac ggccaacctc ctcaacaagc ccggtgaaca
     2281 cgttcctcct ctccacctgc gccgggatag agcgtcacgg cgacgtcctc cccgatcttc
     2341 tcgacctccg agacggagaa ttgcagcgac tccgccatct tcgttgcgcg caggaccccg
     2401 atcatgggga tgccctcgga gccgaggagc ttcggggcgt agaacagggt cagcttgtcc
     2461 gcgagtccct tccgaacgaa gcgcgtcgcg gtctcgccgc caccctcaac gagcagaccc
     2521 ctgatcccac gctcgcccaa ctcatccagg acgaacggga gatccaggcc ctcctcagaa
     2581 cgaggcgtct ccacgacctc gacgccgcat tctttgagac tccgcctgcg ctcgtcggac
     2641 gtgggctccg ccgcaaacac cacgacgggg gcctcgtcgg cgctcctcgc taatttgctc
     2701 tcggggtcgg tcttgaggtg agggtcaagg accacacgag taatacgggg tggccgcact
     2761 ggaagatcac gcgccgtaag catcgggtcg tcagcccgca cggtgttcgc gccgacgagc
     2821 acggcaccgg cctcggccct gagctcgtgt gcgcgacgcc tggcatcctc tccggtcacc
     2881 caccggctgt caccaccgct ggcggcgatc ctgccgtcga gcgtcgtggc gagcttga
//
LOCUS       DATAIR010000517         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_403772, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000517 DATAIR010000000
VERSION     DATAIR010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403772"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1762
                     /locus_tag="VHM69_12350"
     CDS             <1..>1762
                     /locus_tag="VHM69_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX2741230.1"
                     /translation="KLDPQSIPGLPLPRPRFEIFVYSPRTEGVHLRGGEVARGGIRWS
                     DRREDFRTEILGLMKAQTVKNAVIVPVGAKGGFVVKRPPTGREALQQEVVACYKTLIR
                     GMLDITDNISGDEVVPPPDVMRYDDDDPYLVVAADKGTATFSDIANELSAEYDFWLGD
                     AFASGGSVGYDHKEMGITARGAWESVSRHFRELGKDIQNEGFTVVGIGDMSGDVFGNG
                     MLLSRYIKLVGAFNHMHIFLDPDPDPETSFEERERLFRLPRSSWTDYDESLISEGGGI
                     FERTAKSIPLSPQVREMLDVEAESLTPTELINAMLKAEVDLLWNGGIGTYVKASTESN
                     AEVGDRANDTLRANGDDLRCRVVGEGGNLGFTQRGRIEYALRGGRIYMDAIDNSAGVD
                     CSDHEVNIKILLDAIVEDGDMTEKQRNELLAAMTDEVGDLVLRDNYQQTQAISQALTL
                     AHPMVDVHARYIHALEHAGRLNRELEFLPGDEELGERRSDGKGLTAPELAILLSYSKI
                     TTYKDLLNSDAPEDPYLSRELERYFPTPSRERFREQIHEHRLHREITATHVTNSLVNR
                     CGPSFGFRIGEETGAEAPDIA"
BASE COUNT          352 a          592 c          550 g          268 t
ORIGIN      
        1 tcaagctcga cccgcagagc attcccggcc tcccgctgcc acggccccgg ttcgagatct
       61 tcgtctactc cccgcgcacc gagggcgtgc atctgcgggg cggggaggtc gcccgcggcg
      121 gcatccgctg gtcggacagg cgcgaggact tcaggaccga gatcctcggc ctcatgaagg
      181 cccagaccgt gaagaacgcc gtcattgtcc ccgtcggggc gaagggcggc ttcgtcgtga
      241 aacgaccgcc cacgggccgt gaggcgctgc agcaggaggt cgtcgcctgc tacaagaccc
      301 tgatccgggg catgctcgat attacggaca acatctcggg cgacgaggtc gtccctcctc
      361 ccgacgtgat gcgctatgac gatgacgacc cctacctcgt cgtcgctgcg gacaagggca
      421 cggctacctt ctcagacata gccaacgagc tctcggccga gtacgacttc tggctcggag
      481 acgccttcgc ctccggtggc tcagtcggct acgaccacaa ggagatgggg atcaccgcgc
      541 ggggcgcctg ggagtcggtg agcaggcact tccgcgagct cggcaaggac atacagaacg
      601 aaggcttcac cgtcgtcggg atcggggaca tgtccggcga cgtattcggc aacgggatgc
      661 tcctgagccg ctatataaag ctcgtgggcg ccttcaacca catgcacatc ttcctcgacc
      721 cggaccccga ccccgagacg agcttcgagg agcgcgagcg cctcttcagg ctcccccgct
      781 cctcgtggac cgactacgac gagagcctga tctccgaggg cggcggcatc ttcgagcgca
      841 cggccaagtc catcccgctc tcgccgcagg ttcgtgagat gctcgacgta gaggcagagt
      901 ccctgacgcc gacggagctc ataaacgcca tgctcaaggc cgaggtggac ctgctgtgga
      961 acggtggcat cggcacctac gtcaaggcaa gcacggagag caacgccgag gtcggcgaca
     1021 gggcgaacga caccctcagg gccaacgggg acgatctcag gtgccgcgtg gtcggggagg
     1081 gcggaaacct cgggttcacg cagcggggac gcattgagta cgccctgcgt ggaggcagga
     1141 tctacatgga cgccatcgac aactccgcag gcgtggattg ctccgaccac gaggtgaaca
     1201 tcaagatcct gctcgacgcc atcgtcgagg acggcgacat gacggaaaag caacgcaacg
     1261 agctgctcgc cgccatgact gatgaggtcg gtgacctcgt cctgcgcgac aactaccagc
     1321 agacgcaggc gatcagccag gccctcaccc tggcacaccc tatggtcgac gtccacgccc
     1381 gttacatcca cgcactcgaa cacgctggca ggctcaaccg cgaactggag ttcttgcccg
     1441 gcgacgagga gcttggcgaa cggcgctctg acggcaaggg gctcaccgct cccgagctcg
     1501 ccatcctgtt gtcgtacagc aagatcacga cctacaagga cctcctgaac tcggacgccc
     1561 ccgaggaccc gtacctttca cgcgagttgg aacgttactt cccgacacca tcgagagaac
     1621 gattccgaga gcagatccac gagcaccgtc tgcaccgcga gatcacggcc acccacgtca
     1681 cgaacagcct ggtgaaccgc tgcggccctt ccttcggctt ccgtatcggg gaggagacag
     1741 gggcagaggc gccggacatc gc
//
LOCUS       DATAIR010000518         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_34129, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000518 DATAIR010000000
VERSION     DATAIR010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34129"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VHM69_12355"
     CDS             <1..1043
                     /locus_tag="VHM69_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2741231.1"
                     /translation="FYNLMVTDTLHQFRPFYDMHRGFHAFDFIRGQERDLFRPMSPAS
                     EKKMENTLIGGPSAAHAEEIMRQYWANTQGRQSEEDWFAPQVFTKAAEYLEMAKESQP
                     FFMLVDNYDPHEPWDPPEKYVKLYSDDFDGPEPVTSSSGPSDWLTESQLKRMHALYSA
                     EVTMADHWLGHFLDKAQGLGLLDNTMLILLSDHGHAFGEHGFAGKVAGALYPELTDTI
                     FFIRHPSGKGAGETSDHYASTHDVAPTILGAMGHEPHPQMGGQDLSVFLEGKEPGPRP
                     HFTAAYHDHVWARDEKYAMFSLHNGQDPHLFDLENDPKMDKNIASSDPGVVKKMYDEY
                     IVKDAGGPLPSY"
     gene            1078..1692
                     /locus_tag="VHM69_12360"
     CDS             1078..1692
                     /locus_tag="VHM69_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741232.1"
                     /translation="MRRLLSFLLGWSGTLCVPGGVLWALSPVGVYISNYAFHTPNVFW
                     KMFWPAPLLLLLGLIGLQLRQSGSSGLLEKVGFLVTVVGLLMVIAGDVGLYWLGIDDI
                     FILTAPAYRVFRIGLLVLAVGSILFAVTSPRDGALPTWGLLPFVVGSLCGLVSFARDF
                     GSFGAVMWALFGVGWAWLGFSLAIEGFLSFLRTRRTNRGIAAKQ"
     gene            1796..>2312
                     /locus_tag="VHM69_12365"
     CDS             1796..>2312
                     /locus_tag="VHM69_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741233.1"
                     /translation="MGKTRLMQRARSPEALVVLSSAGALLVLGPLRAGLDALPGILFF
                     ASLVLFMAPGLLMSHWFLSELFPGAASLPAAFVFSTGLFGLSGIPMLFVHTSLDAYLL
                     VAGAILAVFLGVALGMVVAGRTVQRDDATHDPDHGWLWVPFLLMCSVLAYAARMRVPH
                     SYNDIWVYLAYV"
BASE COUNT          374 a          756 c          716 g          466 t
ORIGIN      
        1 gcttctacaa cctgatggtc acggacacgc tgcaccagtt caggcctttc tacgacatgc
       61 acagaggctt ccacgcgttc gacttcatca ggggccagga gagggacctc ttcaggccga
      121 tgtcgcccgc ctccgagaag aagatggaga acaccctgat cggcggtccg agcgcggcgc
      181 acgccgagga gatcatgcgc cagtactggg cgaatacgca gggccgccag agcgaggagg
      241 actggttcgc cccgcaggtt ttcacgaagg ccgccgagta cctggagatg gcgaaggaga
      301 gccagccttt cttcatgctc gtggataact acgacccgca cgagccgtgg gacccgccgg
      361 agaagtacgt gaagctttac agcgacgact tcgacggccc cgagcccgtg acgtcgagca
      421 gcggtccctc cgactggctc accgagagcc aactcaagcg gatgcacgcg ctctactccg
      481 ccgaggtgac gatggcagac cactggctcg gacacttcct cgacaaggcg cagggcctcg
      541 ggcttctgga caacaccatg ctgatcctgc tctcggacca cgggcacgct ttcggggagc
      601 acggtttcgc cggcaaggtc gccggagcgc tgtatcccga gctcactgac accatcttct
      661 ttatcaggca cccgtcaggc aagggcgcgg gagagacgag cgaccactac gcctcgacgc
      721 acgacgtcgc gccgacgatc ctcggcgcga tgggccatga gccacacccg cagatgggag
      781 gtcaggacct ctccgtcttt ctggaaggga aggagcctgg tccaaggccc cacttcaccg
      841 ccgcctacca cgaccacgtc tgggcccgcg acgagaagta cgccatgttc agcctccaca
      901 acggccagga cccgcacctc ttcgacctgg aaaacgaccc gaagatggac aagaacatcg
      961 cctcctccga ccccggcgtg gtcaagaaga tgtacgacga gtacatagtc aaggacgccg
     1021 gcggccccct gccgagctat tgatcccgcc cggcgactga tggagggaag aggggttttg
     1081 cgcaggctgt tgtcattcct cctcgggtgg agcggcacac tgtgcgtgcc aggcggcgtg
     1141 ctctgggctc tctctcccgt tggggtctac atctccaact acgctttcca tacccccaac
     1201 gtcttctgga agatgttctg gcctgcgccg ctgctgctcc tgctcgggct gatcggcctt
     1261 cagttgcggc aatcgggtag ttccggccta ctcgagaagg ttgggttcct cgtgaccgtc
     1321 gtggggctgc tcatggtcat agcgggcgac gtcggtctgt actggctcgg gatcgacgac
     1381 atctttatcc tgaccgcgcc ggcgtaccga gtcttcagga tcgggctgct cgtcctcgcc
     1441 gttgggtcca ttctcttcgc cgtcacctca ccccgcgacg gtgcgcttcc cacatggggc
     1501 ctgttgcctt tcgtcgtcgg gtcgctgtgc ggcctggtct ctttcgcgcg ggactttggc
     1561 tccttcggcg ccgttatgtg ggccttgttc ggtgtcgggt gggcgtggct cggcttctcc
     1621 ctcgcgatcg agggcttcct gtctttcttg cgaacgagac ggacgaaccg cggcatagcc
     1681 gcgaaacagt aagtgcccga gaccttcttg gagatcactt gctagaattg caccgaccgg
     1741 acgggctccg gcggcgtcct cgaggcggcg tcacgactgt ctcaaaggga tggtgttggg
     1801 gaagaccagg ctcatgcaac gcgctcggtc gccggaagcg ctcgtcgtgc tgtccagcgc
     1861 gggagccctc cttgtactag gccctttgag ggccggtctc gacgcactac ccggcatcct
     1921 cttctttgcg tccctcgtgc tgttcatggc gccggggctg ctgatgtcgc actggttcct
     1981 gagcgagctt tttcctggtg ccgcctccct gcccgcggcg ttcgtgttca gcactggtct
     2041 cttcgggctc tccggaatcc cgatgctctt cgttcacacc agcctggacg cctacttgct
     2101 ggtcgccggt gcgatcctgg cggtctttct tggcgtggca ctcgggatgg tcgtcgcggg
     2161 gaggaccgtg cagagggacg atgccacgca cgaccctgac cacggctggc tctgggtccc
     2221 tttcctgctc atgtgctcgg tcctggccta cgctgcccgc atgagagtgc cgcactccta
     2281 caacgacatc tgggtctatc tggcatacgt gc
//
LOCUS       DATAIR010000519         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_338441, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000519 DATAIR010000000
VERSION     DATAIR010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338441"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..600)
                     /locus_tag="VHM69_12370"
     CDS             complement(58..600)
                     /locus_tag="VHM69_12370"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX2741234.1"
                     /translation="MPRDALTVEEYMKLEESATVRHEYVGGEIFAMVGATKRHNRIIG
                     NISGCLWGAARGGACRVYSESVKLRVSVDVIYYPDVMVACGPEGADPLVEDDPCLVVE
                     VVSPSTETTDRREKLAAYKRMPGLEAYLIISQDRKWVERHFRDEDGPWRRADLVDEGR
                     FPVPCPESELSLAQIYEGLE"
     gene            complement(734..925)
                     /locus_tag="VHM69_12375"
     CDS             complement(734..925)
                     /locus_tag="VHM69_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEX2741235.1"
                     /translation="MSQLLRPLRYAWEYLKEISGENAYDRYLTVHAATHPGKPPMGRG
                     EFYRWRQDDKYNNPGSRCP"
     gene            complement(932..>2106)
                     /locus_tag="VHM69_12380"
     CDS             complement(932..>2106)
                     /locus_tag="VHM69_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX2741236.1"
                     /translation="PVFAGALFPFVFITIACGALSGFHSLVASGTTPKLIQKESQVRV
                     IGYGAMLTESFVAVMAMIAACILDPGVYFAMNANPALLGDTAQSASQAVSSFGFSVSP
                     ETLTQTAQRVGEDSIIGRTGGAPTLAVGMSQILAGLTGILGTSAQAFWYHFAIMFEAL
                     FILTTVDAGTRVGRFMIQDLIGNVWRPFARTSWLPGNLIASALIVAGWGYFLYAGVND
                     PLGGINQLFPLFGIANQLLAAVALTVGTTILIKMGKLRYAWVTGLPLAWDAAVTLTAS
                     WQKIFSSDPAIGFFAQRAAAAQSIEAGKLNDTLIALKAESIDDAHQILVNTTVDGVLS
                     ILFAVMIIIVIADASRVWFGLIRGSKEPDMSEAPWQESHLDSDGNEIEREPVGAGR"
BASE COUNT          383 a          649 c          677 g          397 t
ORIGIN      
        1 aaggaggctt caggtaacag gtttcaggca tcagctttct tgttgatgcc tgagacttta
       61 ctccaatcct tcgtagatct gcgccaggga aagctctgat tcggggcagg gtaccgggaa
      121 gcggccttcg tctacgagat ctgcccgccg ccaggggccg tcctcgtcgc ggaagtggcg
      181 ctcgacccac ttccggtctt gagagatgat cagatacgcc tcaaggcctg gcatacgctt
      241 gtaggctgcg agcttctcgc gacggtctgt cgtctcggtg ctcggcgaca ccacctctac
      301 caccagacag gggtcgtctt cgacgagcgg atcggccccc tcgggaccgc aggccaccat
      361 cacgtccggg tagtagatga cgtcgaccga tacgcgcagc ttgacgctct cggaatacac
      421 ccgacaagcg ccgccgcgtg cggcccccca gagacaacct gagatattgc ctatgatcct
      481 gttgtgccgc ttcgtagcgc cgaccatcgc aaagatctcg ccgccgacgt actcgtggcg
      541 aactgttgcg ctctcttcga gcttcatata ttcttcgacg gtcagggcgt cgcgcggcat
      601 ggtgggctct gccatgcaac gagtatagcg caagggtatg cctggccaac cgcgcaagga
      661 attggtctga ctctcggcca ggagtttgat ctctagacgt ttccttgccg gccgaccggc
      721 cggcaaggcg tcatcacgga catcggctgc ctgggttgtt gtatttgtcg tcctggcgcc
      781 agcggtagaa ctcgccgcgg cccatcggcg gtttgccggg atgtgtcgcg gcgtggaccg
      841 tcaggtagcg gtcgtaagcg ttctcgccgg agatctcctt caagtactcc caggcgtagc
      901 gcaagggtct cagcagctga ctcatactct cctagcggcc ggctccaacg ggctcgcgtt
      961 cgatctcgtt gccgtccgag tcaaggtgcg actcctgcca tggggcctcg ctcatgtcgg
     1021 gctccttgct gccccggatc aggccgaacc acacgcggga tgcgtctgcg atcactatga
     1081 tgatcatgac ggcgaacagt atggagagca cgccgtcgac cgtcgtgttc accaggatct
     1141 ggtgcgcgtc gtcgatgctc tcggctttga gggctatcaa ggtatcgttg agtttacccg
     1201 cctcgatcga ctgcgcggct gccgccctct gggcgaagaa gcctatcgcg gggtctgagg
     1261 agaagatctt ctgccagctc gcggtgagcg ttacggcggc gtcccacgcg agcggtagcc
     1321 ccgtgaccca ggcgtaacgc agcttgccca tcttgatgag tatggtcgta ccgaccgtca
     1381 gggcgacggc ggcgaggagc tggttcgcga tcccgaagag cgggaagagc tgattgatgc
     1441 cgccaagcgg gtcgttcacg ccggcgtaca ggaagtagcc ccatcccgcg acgatcaggg
     1501 cgctcgcgat gaggttgcca ggaagccagg aggtgcgggc gaagggacgc cagacgttgc
     1561 cgatgaggtc ctggatcatg aaccgcccga cccgcgtacc cgcatcaacg gtcgtgagga
     1621 tgaacagcgc ttcgaacatt atggcgaagt ggtaccagaa cgcctgcgcc gatgtcccta
     1681 agatgccggt caggcccgcg aggatctggc tcatccccac ggcgagggtg ggagcgccac
     1741 cggtacggcc gatgatggaa tcctcgccca ccctctgcgc cgtctgggtg agggtctccg
     1801 ggctcaccga gaaaccgaag ctgctcacgg cctgggaggc cgactgggcg gtgtcgccga
     1861 ggagtgcagg gttggcgttc atggcgaagt acacgccggg gtccaggatg cacgccgcga
     1921 tcatggccat cacagccacg aacgactccg tgagcatcgc cccgtagccg atcacgcgca
     1981 cctgcgactc tttctgaatc aacttgggtg tcgtccccga ggcaacgagc gagtggaagc
     2041 cggagagggc cccgcaggct attgtgatga agacgaacgg gaacaacgcc ccggcgaata
     2101 caggcc
//
LOCUS       DATAIR010000520         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_312183, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000520 DATAIR010000000
VERSION     DATAIR010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312183"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHM69_12385"
     CDS             <1..232
                     /locus_tag="VHM69_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741237.1"
                     /translation="TSLLDRFPILVYAGAALLVYISVEMFFADEAVHHYLEPLVPFEL
                     YIALTAAILFAGIAWLWKRRSEPTAEEDGESS"
     gene            complement(269..814)
                     /locus_tag="VHM69_12390"
     CDS             complement(269..814)
                     /locus_tag="VHM69_12390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2741238.1"
                     /translation="MADPASGGVPSAGCIPGELRTERLRLVTLTPRLVRLALEDRKEL
                     GSELQARVPSSWPGADFARMLPRLADGPDDSSVAAPTRLIVHAADRAVIGEAGFHGPP
                     DRTGTVEIGYSIVPERRGQGFASEATQALIEATFYIPGIRRIIAECLEDNGASLRVLE
                     KLGMRRTRPAGGTVHFELRRP"
     gene            complement(807..1043)
                     /locus_tag="VHM69_12395"
     CDS             complement(807..1043)
                     /locus_tag="VHM69_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX2741239.1"
                     /translation="MPDVTLVVKDVSSVEDAENLERALVRLDPVNLVNVDSEKGLVAV
                     SYEGGEAELNDIRQAVEGAGHGLEDSPGAGNVGG"
     gene            complement(1223..>2083)
                     /locus_tag="VHM69_12400"
     CDS             complement(1223..>2083)
                     /locus_tag="VHM69_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2741240.1"
                     /translation="LGSGVVLGLDDGSATAVTNSHVVRGLQRAQGSGRRRGRAESNGV
                     EVTLSGGGSFPAEVLGDDPPSDLAVVRFEPEEEPPVATLGDAGNLLVGQLVVAIGNPL
                     GFQRSVTAGVVSALGRSIRGESGRLIENVIQTDAAINPGNSGGPLADASGRVVGINTA
                     IIGGAQGIGFAIPVSGTFRRVVFTLVTEGRVRRAYLGVMVQSRPTRARNGFEGGAGVE
                     NVAPNSPAERAGVRPQDVIVGFRDEPVRSNDDLLNLLDASTIGEDTELRVLRGGKEQS
                     LTVRPREQQE"
BASE COUNT          338 a          720 c          644 g          381 t
ORIGIN      
        1 gacctcgctc ctggaccgtt tccccatcct ggtctacgct ggcgcggcgc tgctggtata
       61 catctcggtg gagatgttct tcgccgacga ggctgtccac cattacctcg aacccctcgt
      121 gccgttcgag ctgtacatag ccctgaccgc cgctattttg ttcgccggga tcgcctggct
      181 gtggaagagg cgctcggagc ctactgcaga ggaagatggc gagagttcct gagcccgtgg
      241 ctgcaggcgc ggctaccggg tacgcgctct acggcctacg aagttcgaaa tgaactgtgc
      301 caccagcggg gcgcgtgcgc cgcataccaa gcttctccag aacccttagc gaagccccgt
      361 tatcctcgag gcactcagcg attatgcgcc ttatgccggg gatgtagaac gtggcttcga
      421 tcagggcctg tgtcgcctcc gaggcgaaac cctggccccg gcgttctggg acgatgctgt
      481 agccgatctc aacggtcccg gtcctatcgg gaggaccgtg gaatccagcc tccccgatca
      541 cggctctgtc cgcagcgtgc actatgaggc gggtcggcgc agcgaccgag ctatcatccg
      601 ggccgtcggc gaggcggggc aacatgcggg cgaaatccgc gccgggccac gacgaaggca
      661 cgcgcgcctg gagttcgcta ccaagctcct tgcggtcttc gagggccaaa cgcaccagcc
      721 tgggcgtcag ggttacgagc ctcagccgtt ccgtgcgtag ttcccccggg atgcaaccgg
      781 ccgacggtac gccgccggaa gccgggtcag ccaccgacgt tgcccgcgcc gggcgagtcc
      841 tccaacccgt gcccggctcc ttcgacggcc tgtcgaatat cattcagttc cgcttcgccg
      901 ccctcgtacg agacagcgac gaggcccttc tcggagtcca cgttcaccag gttgacgggg
      961 tccaggcgca ccaacgcgcg ttcgaggttc tcagcgtctt ccacgctcga aacgtccttc
     1021 accaccagcg tcacatctgg catggttcct cccggatagt aggtttcagg tgacaggcat
     1081 caggtttcag cttactaccg ccggggattc cgggtgcgac cgtcgaacgg ctagtgcgcg
     1141 ggttgggggt ctacggtgat gtgactgcca tgcgcgcctg ccatagcttg cctggatctg
     1201 gctcgtacct gagacctgtt gcctactctt gctgctcgcg gggacgcacg gtgagcgatt
     1261 gctccttgcc gccccgcagc acgcgtagtt cggtatcttc gccgatggtg gacgcgtcga
     1321 gcaggttaag gaggtcgtcg ttggatcgga ctggttcgtc ccggaaaccg acgatcacat
     1381 cctgcggcct gacgccagcc cgttcggccg ggctattcgg ggcgacgttc tcgacgccag
     1441 cgccgccctc gaagccgttt cttgcccgcg tggggcgact ctgcaccatc accccgagat
     1501 aggcgcggcg caccctgccc tccgtgacga gcgtgaagac cacgcgccgg aacgtccctg
     1561 agaccgggat ggcgaagccg ataccctgcg cgcccccgat gatggctgta tttatgccga
     1621 ccacccgacc ggaagcgtcg gccagtggac cgcctgagtt gccagggttg atggctgcgt
     1681 ctgtctggat gacgttctcg atcaaacgcc cactctcgcc ccggatggaa cgaccgagcg
     1741 cgctcaccac gccagccgtc acgctccgct ggaaaccgag tgggttcccg atggctacga
     1801 cgagctgtcc gacgagcaaa tttccggcat ccccaagcgt ggcgacaggc ggctcctcct
     1861 ccggctcgaa gcgcaccacc gccaggtcgc tcggcggatc gtcccccaga acctcagccg
     1921 gaaacgaacc accaccggag agcgtcacct cgacgccgtt ggattccgcc cgacccctcc
     1981 tgcggccact gccctgtgcc ctttgcaggc cgcgcaccac atggctgttt gttaccgcgg
     2041 tcgcggagcc gtcgtccagg ccgagcacga ccccgctccc gag
//
LOCUS       DATAIR010000521         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_34913, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000521 DATAIR010000000
VERSION     DATAIR010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34913"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..>1672)
                     /locus_tag="VHM69_12405"
     CDS             complement(179..>1672)
                     /locus_tag="VHM69_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741241.1"
                     /translation="AIERELREAGVRRERRRAEDALRVSETRFRLMIEQSPLSIQIFS
                     PDGSTLRVNRAWEELWGVTLDQIPGYNVLQDPQLVEKGIMPYIERGFAGEAAAIPPVM
                     YEPDETIPSVSGVAYRWVQAFIYPVIDADEEIQEVVLVHEDITDRKEAEEERRRAEEK
                     YRSIFENSVEGIFQTTVNGRFLTANPAMARMFGYASPEELLGAISDIGDQLYVEPRRR
                     EEFYELALRDGFVSGFEIQMLRKDGSPMWASVNARAVYDAGGHIAGYEGTVEDITERK
                     RAEEALREIREAERRRIARELHDVVLQDLTYALQSMQVLRRRPEGVDRAGETSRQIEA
                     LKRAVGGLRDAIYDLRLESDREWPLVRAVQSIVELNRQISDGCEFELVVDEGFPATIS
                     GAAGIEVVRVVQEALANVRRHSEASHAAVTLGVVNGDVLVEIEDDGRGFGPETSYGMG
                     LTGMRERVLVLGGNLEVEGREGLGTRVFLRVRLAALSGGEAGKLSNT"
BASE COUNT          273 a          599 c          486 g          314 t
ORIGIN      
        1 gcctgggcga ctacctcgaa ctcgggttgc tgatcgaaga cgaaggcaag cgtctgacgg
       61 aacgaggcgt ggtcctctag aagcaagatt cgctgcattt cctccctttc gcggaccttt
      121 cccgcggatc ccaggcgcaa aacaatatac cagctttgac ggcgtggaga ccgccggctc
      181 aggtgttcga aagtttcccg gcttccccgc ccgacagggc ggcgagcctc accctgagaa
      241 acaccctggt tcccaggccc tccctgccct ccacctccag gttcccgccg agcacgagga
      301 cgcgctcgcg catccctgtg agccccatcc cataagaggt ctccgggccg aaccccctgc
      361 cgtcgtcctc gatctccacg aggacgtcgc cgttcacaac accgagggtg accgcagcat
      421 gcgaggcctc cgagtggcgc ctgacgttcg ccagtgcttc ctggacgacg cgcaccacct
      481 cgataccggc ggcccccgag atcgtggccg gaaacccctc gtcaacgacc aactcgaact
      541 cgcagccgtc gctgatctgg cggttcagct cgacgatgga ctgcacggcg cggacgagcg
      601 gccactccct atcgctttcg agccgcaggt cgtagatggc gtcgcgcagg ccccccacgg
      661 ccctcttgag cgcctctatc tggcggctgg tctctccggc gcggtcgacg ccctccggcc
      721 ttctcctgag aacctgcatc gactgcaggg cgtaggtgag gtcctgcaag accacgtcgt
      781 gcagctcgcg cgcgatccgg cgtcgctcgg cctccctgat ctcgcgcagc gcctcctcgg
      841 cccgcttgcg ctcggtaatg tcttctaccg tgccttcgta ccctgcgatg tggccaccgg
      901 cgtcgtagac ggcccgtgcg tttaccgagg cccacatcgg actcccgtcc ttgcgcagca
      961 tctgtatctc gaagccgctc acgaagccgt cccgcagcgc gagctcgtag aactcctccc
     1021 tgcggcgggg ctcgacgtag agctggtctc ctatgtcgga gatggccccg agcagctcct
     1081 ccggagaggc gtagccgaac atgcgggcca tcgcgggatt cgccgtcagg aaccgaccgt
     1141 tgactgtagt ctggaagatc ccttcgacag agttctcgaa gatcgaacgg tacttctcct
     1201 ctgcccgcct gcgctcctcc tcggcctcct tgcggtccgt gatgtcctca tggaccagca
     1261 cgacctcctg gatctcttca tcggcatcta tgacaggata gatgaaggcc tgtacccagc
     1321 ggtaggcgac gccgctcacg ctcgggatag tctcatcagg ttcgtacatg acgggaggaa
     1381 tggctgcggc ctctccggcg aagccccttt cgatgtaggg cattatgccc ttctcgacga
     1441 gctgcgggtc ctgcaacacg ttgtatccag gtatctggtc gagcgtcacg ccccagagct
     1501 cctcccacgc ccggttcacg cgcaacgtac tgccgtcggg ggagaagatc tggatgctca
     1561 agggggactg ttcgatcatc agcctgaaac gcgtctcgga gacgcgcagc gcgtcctccg
     1621 ccctccgacg ctcgcggcgt accccggcct ccctgagctc ccgctcgatg gc
//
LOCUS       DATAIR010000522         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_312318, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000522 DATAIR010000000
VERSION     DATAIR010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312318"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VHM69_12410"
     CDS             complement(<1..979)
                     /locus_tag="VHM69_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741242.1"
                     /translation="MKRLEIVQIGLGHVGRAVAQIVLEERKRWLERRGIDVRYRAVSD
                     TSGALVGEDLLPQAIRLKEEGGRLAELGVEPLEGVLDSEPEPGYTRVVVDVAVHGGTF
                     DLDLLGIRGGAYLVLSNKGPLSGSTAQYLQLVRMIPDRLWHEATVGAGMPIISTIGGL
                     IETGDEVLEIQASPSGTLGFIMSAVESGRSFSEAVREAVALHYAEPDPRDDLSGLDVA
                     RKAIILARMMGREIEPEEIPYESLVPEGLEDVPLEEFMQRLPEHDEAFAERLLAVEAH
                     HMLRYLVRIPKEGRVTVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTL"
     gene            complement(976..2211)
                     /locus_tag="VHM69_12415"
     CDS             complement(976..2211)
                     /locus_tag="VHM69_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2741243.1"
                     /translation="MSSLEKPLVIKFGGTSVGGGAEFVRAARIAAGAAMERPVAVVVS
                     AMSGTTDTLLGYASMSSSWVRQTLTRETHGGSIAELRRVLAERHLRAAREAVAPRRLP
                     GVEGRIVDLLGELEEVIEAPSDNLKARRDQIAVYGERLSAEILAGAIGNQGAPSEAVA
                     ADPIATDGRFGEAEVDPAATRERTGRHVSPLLERGLVAVVPGYVGHSPEGLPTTLGRG
                     GSDLSATVLGRALGSDEVWIMSDVDGVLDADPRLVPDASLMPRLSYREAGVFAGLGAK
                     VLHPRTMEPASEAGIEVYVRNTFAPEGAGTHVTGFDPGAGVRCVALRQNVAVEIPCTD
                     GREGRAGMVVCIGSPEEEDLARGRRVLREAGIAPLHFGIAAAGLVFVVPAEAGMDSLR
                     ALHAGLIPPEGHFAGEEVA"
BASE COUNT          383 a          815 c          783 g          401 t
ORIGIN      
        1 tgagcgtaac atccgagtag cggcgcgtgc ggaagtcgaa gacgttctcg gggccgctga
       61 tagggccgaa ggggctctcc acgggtgtgt cctgcaggcc gaccgtgacg cggccctcct
      121 tcgggatgcg gaccaggtag cggagcatgt ggtgtgcctc gacggcgaga aggcgctccg
      181 cgaaagcctc gtcgtgctcg gggaggcgct gcatgaactc ttccagaggg acgtcttcca
      241 ggccttcggg gaccagcgac tcgtagggga tctcctccgg ttcgatctcc cgccccatca
      301 tcctcgcgag gatgattgct ttgcgtgcca cgtccagccc cgagaggtcg tcgcgggggt
      361 ccggctccgc gtagtggagc gcgacagctt ccctcacagc ctcggagaag gagcggccgc
      421 tttctaccgc gctcatgatg aagccgaggg tgccgctcgg gctggcctgt atctcgagca
      481 cctcgtctcc cgtctcgatg aggccgccaa ttgtcgagat gataggcatg ccggccccta
      541 cagtcgcctc gtgccagaga cggtcgggga tcattcgcac gagctgcagg tactgggcag
      601 tgctccctga gagcggtccc ttattggaga gcaccagata agcccctccg cgtataccga
      661 gcaggtcgag gtcgaaggtt ccgccgtgga ccgccacgtc gacgaccaca cgcgtgtagc
      721 cgggctcggg ctccgagtcg agcacgcctt ccagcggctc gacaccgagt tcggcgagcc
      781 ttccgccttc ctccttgagg cgaatcgcct gcggcaggag gtcttccccc acaagcgcgc
      841 cggaggtgtc cgagaccgca cggtacctca catcgatacc acggcgctcc aaccagcgtt
      901 tgcgctcttc caggacgatc tgggcgaccg cgcggccaac gtgcccgagg ccgatctgga
      961 cgatctcgag tcgcttcacg cgacctcttc gccggcgaag tgtccttctg gtgggatcag
     1021 gccggcgtgc agagcgcgca atgagtccat acccgcttcg gcaggcacga cgaaaaccag
     1081 ccctgccgcc gcgatgccga agtggagggg cgcgatgccc gcttcgcgca agacacgcct
     1141 tccacgggcg agatcctcct cttcaggcga cccgatgcag accaccattc ccgcgcgtcc
     1201 ctcgcggccg tcggtacatg ggatctcgac ggcgacgttc tgccgcagcg cgacacagcg
     1261 gacgcccgcc cccggatcga agcccgtaac gtgggtgccg gcaccttccg gcgcaaacgt
     1321 gttgcggaca taaacctcga taccggcctc ggaggctggc tccatcgtcc tcgggtgcag
     1381 gaccttcgcg ccgaggccgg cgaagacgcc cgcctctctg tacgagaggc gaggcatcag
     1441 agaggcgtca ggcaccaggc gcgggtcagc gtcgaggacg ccgtccacgt ccgacatgat
     1501 ccagacttca tcagatccca gcgcccgacc gagcacagtc gccgagaggt ccgagccacc
     1561 tctccccagc gtcgtcggca gcccctcggg tgagtgcccg acgtagccgg gcacgaccgc
     1621 cacaagccct ctttcgagca gcgggctgac gtggcgcccc gttcgttcgc gtgtggctgc
     1681 gggatctacc tcggcctctc cgaagcgtcc gtccgtggct atgggatcgg cggcgacggc
     1741 ctcggatggc gcaccctggt tgccgatcgc cccggcgagg atctccgccg aaaggcgctc
     1801 tccgtagacc gcgatctgat cgcggcgtgc cttgaggttg tcggagggag cctcgatgac
     1861 ctcttctagc tcgccgagca aatcgacgat gcgcccttcg acgccgggca gacgtctcgg
     1921 ggccacggcc tcgcgggctg cgcgcaggtg gcgctcggcg agcacgcggc gaagctcggc
     1981 gatggagccc ccgtgcgtct cgcgcgtgag ggtctgtctt acccaactcg agctcatgct
     2041 ggcgtagccg agcagggtgt cggtcgtacc gctcatcgcc gagacgacta ccgccactgg
     2101 tctctccatg gccgcgcccg cggcgatacg ggcggcgcgc acgaactcgg cgccgcctcc
     2161 gacagaagtg cccccgaact ttattacgag aggtttctcc aatgagctca cgctccgttc
     2221 agtgcctgat ccaggtcgta caggatgtcg tcgatggact cgaggcccac ggagagcctg
     2281 acgtagtcgt cgctgacgcc ggtggacttc tgctcgtcca ccgagagctg cgagtgggtc
     2341 gttgaggccg ggtgaatgac caggctcttg gcgtccccaa cg
//
LOCUS       DATAIR010000523         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_450345, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000523 DATAIR010000000
VERSION     DATAIR010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450345"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VHM69_12420"
     CDS             <1..900
                     /locus_tag="VHM69_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2741244.1"
                     /translation="VVLQPEDIVMGFAFPEADEAVERTLRMARERGALTFALTGEAGE
                     YSFAKPDGDGFVTQEIFEVLYHLLWETVHVYFEHREQGHDVGSSSFLYPFLGKGKQPL
                     EEVVREVQGSMLQKMQEVNRLRAAAAESEAGAISAIAVTVAERLERGGKLITFGNGGS
                     ATDANDLVSDCVDPPPGFGTIPAVSLAAESANITAIANDIGTEAIFTRQLIAHARPED
                     VAVGISTSGGSANILAALAEARKRGLLTVGIVGYDGGRIVSERLADHTVVVRSDYIPR
                     IQEVQASIYHVLRGLIDQVLTKS"
     gene            1070..>1921
                     /locus_tag="VHM69_12425"
     CDS             1070..>1921
                     /locus_tag="VHM69_12425"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX2741245.1"
                     /translation="MNAREKLAMDRSARLMGRAVGLMPGGVNSPVRAFRSVGGDPLFM
                     ERGEGSRIFDVDGNSYIDYVMSYGPLVLGHSYPEVIEAIETAARKGTTFGAPTELEVE
                     LASLVCGAVPSVEMVRMTNSGTAATMSAIRLARGYTGRERILKFDGNYHGHGDALLVS
                     AGSGVATLGLPDSAGVTKGAAKDTAVLPYNDLDAVRGLFEREGEEFAAVILEPVAGNM
                     GCVPPAEGYLEGLREITHQHGALLVFDEVMTGFRVARGGAQERYGVVPDMTTLGKVIG
                     GGLPVGAY"
BASE COUNT          351 a          558 c          665 g          347 t
ORIGIN      
        1 gtcgtgctac agcccgagga catagtgatg ggcttcgcgt tccctgaggc ggacgaggca
       61 gtcgagcgga cgctacggat ggcgagggag cggggggcgc ttactttcgc cctgacgggg
      121 gaggctgggg agtactcttt cgccaagcct gacggggacg ggttcgtgac acaggaaatc
      181 ttcgaggtcc tctatcactt gctctgggag acggtccacg tttacttcga gcatcgcgaa
      241 cagggccacg acgtgggcag ctcctcattc ctgtatccat tcctgggaaa gggcaagcaa
      301 cccctggaag aggtcgtgcg cgaagtgcag ggctcgatgc ttcagaaaat gcaagaggtg
      361 aaccgcctgc gcgcagcggc cgcagaatct gaggcagggg cgatctctgc gattgcggtt
      421 accgtcgccg aacggttgga gcggggaggc aagctcatta ctttcggcaa cggtgggtcc
      481 gccacggacg cgaacgacct ggtctcagac tgcgtggacc cgccgcctgg gttcggcacg
      541 ataccggctg tctcgctagc tgcggagtcg gcgaacatta cggccatagc caatgacata
      601 ggaaccgagg cgatcttcac ccgccagctc atcgcccacg cccgtcccga ggatgttgca
      661 gtcgggatct cgacgagtgg gggatctgcc aacatcctcg cagccctcgc cgaggcccgc
      721 aaacgagggc tgcttacggt cggcatcgta ggctacgacg ggggcaggat cgtgagcgag
      781 cggctcgccg atcatacagt agtcgtccgc tcggactaca tccctcgcat ccaggaggtc
      841 caggcttcta tctaccacgt actgcgcggc ctcatcgacc aggtgctgac aaagagttga
      901 cccgtggcgc acgtgcgatg gcgagtggta taaggtgtta cacggcggcg ccggccgcta
      961 ctgtatagtc tgcgagagaa gagctagtgt ccatgttcgg cctcgcgtat gttagcttct
     1021 gacggtgcgc gatcttgaag ggggtaggct cgacatcagg gattccatcg tgaacgcaag
     1081 ggagaagctc gccatggacc gttcggcaag gctgatgggg cgggctgtcg ggctgatgcc
     1141 aggtggcgtg aacagccccg tgcgggcgtt ccgctccgtc ggaggggacc cgctgttcat
     1201 ggagcgcggc gagggctcgc gcatcttcga tgttgacggc aactcgtaca tcgactacgt
     1261 gatgagctac ggtccgctcg tcctggggca ttcttatccc gaggtgatcg aggccataga
     1321 gaccgcggcc cgaaagggaa ctactttcgg cgccccgacg gagctcgagg tcgagctcgc
     1381 atctctggtg tgcggggccg tcccttccgt cgagatggtc aggatgacca actcggggac
     1441 agcggcgacg atgagcgcca tccggctcgc acgcgggtat acggggcggg agaggatcct
     1501 caaatttgac ggcaactacc acggccacgg agacgcactc ctcgtctccg ccggctcggg
     1561 cgtggcgact ctcgggctcc ctgacagcgc cggcgtcacg aagggggcag ccaaagatac
     1621 cgccgtcctg ccgtacaacg acctggacgc ggttcgaggc ctcttcgaga gagagggaga
     1681 ggagttcgcc gccgtcatcc ttgagcctgt cgcggggaac atggggtgcg ttccgccggc
     1741 agaagggtat ctcgaaggcc tcagggagat cacgcaccag cacggagcct tgctcgtctt
     1801 cgacgaggtc atgaccggtt tcagggtcgc tcgtggcggg gcgcaggagc gttacggcgt
     1861 cgtgcccgac atgacgacgc tgggaaaggt tatcggtggt gggctccctg tcggggcgta
     1921 c
//
LOCUS       DATAIR010000524         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_128054, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000524 DATAIR010000000
VERSION     DATAIR010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128054"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VHM69_12430"
     CDS             <1..797
                     /locus_tag="VHM69_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741246.1"
                     /translation="REIVDGGVSVVVPEPGESVFAEAMSPRGGEHLVVSNLAGKTLVL
                     GEVGSESGTQDTFTTQSSGPGACIDAHRNSWDSRLYNYLRYYYNRRSTPSHLADVGVA
                     RAIERAGRNVSRVRDSCGIQDRVPAVLKYRGVTRTSVDMDAGGTCKANDRKSVVGFGD
                     LPRGYTGNTCVWSWIVDGPDRINSADVRLNKADYRWTTRVTRSCRGRHDVESTMTHER
                     GHTFGLGDVSDASHPNLTMSSRSNGACQISERSLGKGDAIGLNEKY"
     gene            complement(1044..1802)
                     /locus_tag="VHM69_12435"
     CDS             complement(1044..1802)
                     /locus_tag="VHM69_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX2741247.1"
                     /translation="MEITVVGSGTVVPRLHRRQSCVVVRAGEETLVFDLGSGAVRGML
                     HSDLDPFDVDRIYFSHFHPDHTVDVVPLLFAIKYGAREERTRPLYVTGPEPFLQFWDS
                     LMEVWGEWMAGDYATLVSELPIECPSPIDLAGCLLSWAPAEHRPESIAYRLDGDGGAF
                     VYTGDTEYSQSVVDLSRGAHTLLIECSFPDDAPIPGHLTPSGVARIARESGVGRVVIT
                     HLYPAVDNSSLVSEVQRNYDGEILVAEDGLKFTV"
     gene            complement(1804..2412)
                     /locus_tag="VHM69_12440"
     CDS             complement(1804..2412)
                     /locus_tag="VHM69_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2741248.1"
                     /translation="MKSENRLVLLLSTAAFVVCTAAAGAGLFRRMDARMLYLTQREPS
                     QLLDAIGLVTSVAGGVEFVAVAAFALATGLFVDGQRRLAFRLLTAFVVTGLIEVALKM
                     VVPQAPVPDGVARGPDPSLLDVSTPYPYPSGHFLRAVLLLGAVYVLWPNRIGRGAILV
                     FLAASAASRVYLGTHWPSDVLGGALLGVAGLAWAFGQNKKGP"
     gene            complement(2495..>2713)
                     /locus_tag="VHM69_12445"
     CDS             complement(2495..>2713)
                     /locus_tag="VHM69_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX2741249.1"
                     /translation="TAHLKEAIERLPERARYVLVRRYGLDDREPATLAELGEELDISR
                     ERVRQLQREAERILKGGEYGRVLRDAVA"
BASE COUNT          539 a          835 c          886 g          453 t
ORIGIN      
        1 gaagggagat agttgatggc ggtgtctccg tggtcgttcc cgagcctggg gagagcgtct
       61 tcgccgaggc gatgtcgccg cggggcggcg aacacctggt ggtcagcaac cttgctggca
      121 agacgctcgt cctgggtgaa gtcgggagcg aatcagggac ccaggatacg tttacgacac
      181 agtcctccgg accaggagcg tgcatagacg cgcaccgcaa ttcgtgggac tcgcgactgt
      241 acaactatct ccgctactac tacaaccgga ggagcacgcc ctcccacctg gcagacgtgg
      301 gggtagcgcg cgcgatagag agagctggcc gcaacgtcag cagggtgagg gacagttgcg
      361 gcatacagga cagagtgccg gccgtcttga agtaccgggg agtcacacga acatccgttg
      421 acatggacgc gggcggcact tgcaaagcca acgaccgcaa gagcgtcgta ggcttcggag
      481 accttccaag aggctacacg gggaatacct gcgtgtggtc ctggatcgtg gatgggcccg
      541 ataggatcaa ctccgcggac gtgcggctga acaaggccga ctatcgctgg acgacgaggg
      601 tgaccagatc gtgcaggggc aggcacgacg tggaatccac gatgacccac gagcgcggcc
      661 ataccttcgg cctgggagat gtctccgacg cttctcatcc gaacctgacg atgagctcca
      721 ggagcaacgg tgcctgccag atttcggagc gcagcctcgg gaaaggcgac gccatcggcc
      781 tgaacgagaa gtattgacct ctgctattcg cgctggtcag ctcgctacgc cattcgggtt
      841 tcgctgtcag ttattgatgc tttgtttttg gtggctgcct ctttcttgac gggacctgta
      901 atctcgagaa acgcttcagt agtggatgcc ggtcagatgt ctgggtcagc gaccgttgtc
      961 actcaaagcc tgactgctga aacctgatgc ctgtagcccg ccatgctgaa tacgttcagc
     1021 acggcgctgc gagcgcggtg cgactataca gtgaacttca ggccgtcttc ggccactaag
     1081 atctcgccgt cgtagttcct ctgcacttcc gagaccaggg aagagttatc cacggccgga
     1141 tagaggtggg tgatgacgac ccttccgacg cctgactcgc gcgcgatcct cgctacgccc
     1201 gacggtgtca ggtggccggg aatgggcgca tcgtccggga aggagcactc gatgaggaga
     1261 gtatgggcgc cgcgcgagag atctaccact gactggctgt actcggtgtc gcctgtgtag
     1321 acgaacgcgc cgccgtcgcc gtcgagccgg taggcgatgc tctcgggacg gtgctccgca
     1381 ggagcccaag agagcaggca gcccgcgagg tctatagggg aggggcattc gatgggtagc
     1441 tccgagacga gggtagcgta atcgccggcc atccactcac cccatacctc catcaaagaa
     1501 tcccagaact ggaggaaggg ctcggggccg gtcacgtaca gtgggcgggt tcgctcctcc
     1561 cgcgcaccgt atttgatggc gaagagaagc ggaaccacgt ccaccgtatg gtcggggtgg
     1621 aagtgggaaa agtagatgcg gtccacgtcg aaagggtcga ggtcggagtg gagcatgccg
     1681 cgtaccgccc cggagccgag gtcgaagact agcgtttcct ccccggcacg caccacgacg
     1741 cagctctggc gcctgtggag ccggggaact accgtgcctg atcctacgac cgtgatctcc
     1801 aagtcagggc cctttcttgt tttgtccgaa cgcccacgcg agtcctgcga cgccgagcag
     1861 ggctccaccc agtacgtcgg aaggccagtg tgtcccgagg tagaccctgc tcgctgccga
     1921 tgcggccagg aagaccagga tcgcgcccct cccgatcctg ttcggccaca ggacgtagac
     1981 ggccccgagc aggagtaccg cacggaggaa atgcccgctg gggtacggat aaggagtgct
     2041 cacatcgagc aacgaggggt ccggcccccg cgcgacccca tcaggcacgg gcgcctgcgg
     2101 caccaccatc ttgagtgcga cctcgatcag ccctgttacc acgaacgccg tgagcagccg
     2161 aaatgcgagg cgacgctgac cgtccacgaa taatccagtg gccagcgcga acgcggcgac
     2221 ggcgacgaac tcgacacctc ccgctacgga cgtaaccaat ccgatcgcat ccaggagctg
     2281 cgagggctcc ctctgtgtca ggtagagcat gcgcgcgtcc atgcggcgga acagccccgc
     2341 gccggcagcc gcggtgcaaa ccacgaaggc cgctgtagag agcaggagaa ccagcctgtt
     2401 ttcgctcttc acgtcctcac agactagcaa agcgtgggcg gcggggcacg agaaaggccg
     2461 gacccgaagg cccggccagg tcagcaagaa cgagtcaggc tacggcgtcg cgcaggacgc
     2521 ggccatactc gccacccttc aggatgcgtt ccgcctccct ctgcagctgc ctcacccgct
     2581 cgcgcgagat gtcgagctcc tcgccgagtt ccgcgagggt ggcgggctca cggtcatcga
     2641 ggccgtagcg cctgacgagc acgtagcgcg ccctctcggg gagacgctcg atcgcctcct
     2701 tcagatgtgc cgt
//
LOCUS       DATAIR010000525         8587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_404928, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000525 DATAIR010000000
VERSION     DATAIR010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404928"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1109
                     /locus_tag="VHM69_12450"
     CDS             90..1109
                     /locus_tag="VHM69_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX2741250.1"
                     /translation="MKGLHVYSDPASERHVNHLAGVEAPERYPAALAGVESAERSGVP
                     VEWRPCAPAEAGALAAVHDPAYVEHLRELSAEGGGYLSLDTAVGAGSWEAASLASGAA
                     CSAVESAIAGETAFSIARPPGHHAGRDYGMGFCLTNHVAVAAGYALYRGLGRVAILDW
                     DVHHGNGTQDIFYDEDRVLYLSSHQSPFYPGTGKADEVGEGEGLGFTANVPLPGGSGE
                     DIYSEVFAGVFVPVLKEFMPDVILVSAGFDAHADDLLGGMALGSDSFGRFAALISGLS
                     REVGAAPPAFVLEGGYNLAALTASVDATIRGVYDEPPDWEYEGGVRAVEKSHENLAPF
                     WRSLR"
     gene            1114..1788
                     /locus_tag="VHM69_12455"
     CDS             1114..1788
                     /locus_tag="VHM69_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX2741251.1"
                     /translation="MRIFGFPPFEDRRDAGRRLARKLDRFKDERPVVFALPRGGVPVG
                     YEVSHSLEAPLDVFVSRKLGAPGQPEFGIGAVASGGVRVLNEDVVRRLGIPEEYVEWI
                     TARETAEVERRLRYFRGERPEPDVGGRTAILVDDGLATGVTARAAVEALRQRRPERLV
                     LAAPVCAFQTADLFRPIVDELVCLELPSNLGAIGFWYRNFDQTSDEEVVELLERARSE
                     RGTGEL"
     gene            complement(2098..2580)
                     /locus_tag="VHM69_12460"
     CDS             complement(2098..2580)
                     /locus_tag="VHM69_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2741252.1"
                     /translation="MAEGVTLNYNDPEFFERYRRMRESPASANELIEQPALRACLPPL
                     EDLEVLELGCGMGQLSLYMAEQGASRVLATDASERMLGVARSERQHPRIEYLKSATED
                     LDPPQESFDLVASSLAMHYVADYDSLVRSVASWLRSGGWFVFSVEHPMKTAPKAPGVD
                     "
     gene            2681..3340
                     /locus_tag="VHM69_12465"
     CDS             2681..3340
                     /locus_tag="VHM69_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX2741253.1"
                     /translation="MCGRYTLSTPAGRLAEAFQLDDGAVDLTPSYNVAPTQEVAAVLE
                     EDGKRRLEMLRWGLVPSWADDPEIGARMINARSETAPQKPSFRRAFRSMRCLIPADGF
                     YEWKRENGGKQPYYFHMHDGRPFAFAGLWERWEKGGELLTCAILTTKPNSVLEGIHDR
                     MPVILPSGAYDAWLDPDADREQLIDLMIPYPGDDLETYPVSRFVNSPRNNDERCIEPA
                     A"
     gene            3337..4251
                     /locus_tag="VHM69_12470"
     CDS             3337..4251
                     /locus_tag="VHM69_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX2741254.1"
                     /translation="MSVDVRADVREALREGAPVVALESTLISHGLPYPQNLEVAIEAE
                     RAVRTEGAVPATIGVIDGVAKVGLDEAGLVLMATAEGIPKFSARDLPTAVAKGSHGAT
                     TVAATAQLAALAGIRLFATGGLGGVHREARQSWDVSADLAALARTPVAVVCSGVKSIL
                     DVPATLEYLETLGVPVVGYRTRRFPGFYLTDSGSPLDWAVESAEEAARTIKAMKSLGF
                     EGSGLVVANPLSEEDQLDPPLHDRALRTSLEELKRRGVSGKDVTPFLLDRFAKETKGE
                     SLRANRLIIINNAGLAARIAVALSAGRA"
     gene            4252..5142
                     /locus_tag="VHM69_12475"
     CDS             4252..5142
                     /locus_tag="VHM69_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX2741255.1"
                     /translation="MKSPRVVVVGDLLYDLLARVEEDVTLGTDTFTPIRAVAGGSGAN
                     AAAWLASAGVETHFVGRVGDDVFGRFLERELERVGVKAHLSGDPSMATGKVFVLVDGA
                     GERTMITDRGAGENLCSEDLPGSHFSSGHLHLSGYTFSGGSRRETARKALRLARESGM
                     TVSVDPSSVPLLADVGPERFLGWTSGADLCFPNLAEGSLLAGTEDPDLIAERLLDHYS
                     GVVLKLGAEGAMYVGAEGGRASMPAASARVIDTTGAGDALCAGFLAAWLVGATPAEAL
                     RRGVGLAASAVGRIGGRPDA"
     gene            5287..5994
                     /locus_tag="VHM69_12480"
     CDS             5287..5994
                     /locus_tag="VHM69_12480"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2741256.1"
                     /translation="MKLAVVVLVSTLVLLVAACGRVSSGHSSDDSGKQEASGIPEQST
                     TAEEATVEDGASGEAAELARAEVGKKEISNMIPADGKKPDAARPLPEDPPRGIEVYPA
                     TTNHTVRGPIEYDRKPPTNGDHDPLWQNCGFYEKPLKDRHAVHSLDHGVVWVTFRPDL
                     PKRQIKPLRSYGRENYVIVSPYPDQDAPVIVTSWRVQLELNGADDPRLRKFVNQFRIS
                     EIAPLSGNRCTLGIGDR"
     gene            complement(6033..7385)
                     /locus_tag="VHM69_12485"
     CDS             complement(6033..7385)
                     /locus_tag="VHM69_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX2741257.1"
                     /translation="MQQTKFILDEKAIPESWYNLVPDLPFGLEPPLNPATREPVGPEA
                     FAPIFPQAIIEQEVTSDSYVPIPEEVREIYALWRPTPLFRARRLEKILDTPAHIYYKY
                     EGGSPTGSHKPNTAVPQAYYNREEGVRRLTTETGAGQWGSSLAFACRQMGLDCTVYMV
                     RVSYDQKPYRRIMMETYGAEVHASPTDLTQAGRNILEEHPDSPGSLGIAISEAIEDAV
                     KNDDAKYSLGSVLNHVLLHQTVIGQEALQQMELAGEYPDIVIGCVGGGSNFGGVAFPF
                     IRENLKNGKNTRVVAVEPASCPTLTKGRYTYDFGDTAGTTPMMKMYTLGHSFVPSGIH
                     AGGLRYHGDSPIVSALVHEGLVEARAYAQNPTFEAGVAFARAEGIVPAPEVTHAIKAA
                     MDLALEAKETGEQKTILFNLCGHGHFDFSAYEQYLAGELVNLEHSDEEIEAAVAGIPG
                     "
     gene            complement(7538..>8587)
                     /locus_tag="VHM69_12490"
     CDS             complement(7538..>8587)
                     /locus_tag="VHM69_12490"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX2741258.1"
                     /translation="DLYNGTITPELATEIVPGRETLTSISDRTDSLAAINGGYFVIGD
                     NDGTPGDLAGISVIDGTLVSEAVDGRTSLILPPGSGEGADIAAISDTITATSSDGATR
                     EVDGLNRKPGLIRGCGGDGGDSPTEQPKHDFTCADSSELILFTPDFGTSTEPGPGVEA
                     VLDSSGRVVKLRKSRGGPIPENGSVLSGTGEAADWLRAHARVGRTINISTSITADGAP
                     LAPGTSVVNGGPRLLSNGSPDITAYAEGFVWPENPEFYYRFGVRRNPRTLAGVTPEGN
                     LLLVAVDGRQPGYSVGASFEESALIMQALCAEEAVNLDGGGSTSLTIGEELMNSPSDP
                     GNVERPIGDAVVLLP"
BASE COUNT         1488 a         2707 c         2858 g         1534 t
ORIGIN      
        1 ctctgacgtg gccggccttg cctgggatga tgctcacccc cgataataga aacccgaacg
       61 gcgcggaaat ggaagcgtga cctgggagcg tgaaaggttt gcacgtctac agtgacccgg
      121 cctcggagcg ccacgtcaac cacctagcag gcgtggaggc gcctgagagg taccccgcgg
      181 ccctcgccgg cgtcgaaagt gcggagcgta gtggtgtacc cgtggagtgg cgcccgtgcg
      241 cacctgcgga ggcaggagcc cttgccgcgg tccacgaccc ggcttacgtc gaacatctgc
      301 gggagttgtc ggcggaggga ggaggctatc tcagcctgga tacggccgtt ggagcgggct
      361 cttgggaggc cgcctcgtta gcgagtgggg ccgcatgctc cgccgtcgag agcgccatcg
      421 ccggcgagac ggccttctcc atcgcgcgtc cccccggaca ccacgcgggg agggactacg
      481 ggatggggtt ctgcctcacc aaccacgtcg ccgtcgccgc cggttacgcg ctctacagag
      541 gtctcggtcg ggtcgccatc ctcgactggg acgtgcatca cggcaacggc acccaggaca
      601 ttttctacga cgaggacagg gtactttacc tctcgtccca ccagagtccc ttctatccgg
      661 ggacaggaaa agccgacgag gtgggcgagg gagaagggct gggcttcacg gcgaacgtgc
      721 cgcttcctgg gggctcgggg gaggacatct attccgaggt cttcgcgggt gtcttcgtgc
      781 ccgtattaaa ggagttcatg cccgacgtga tcctcgtctc cgccggtttc gacgcccacg
      841 cggacgacct gctcggtggg atggctttgg ggagcgactc cttcgggcgc ttcgcggccc
      901 tgatctccgg cctctcccgc gaggtcgggg ccgctccccc cgcgttcgta ctcgaaggag
      961 gctacaacct cgccgccctc acagcgagcg tcgacgcgac gatacggggt gtctatgatg
     1021 aacccccgga ttgggagtat gagggtggcg tgcgggcggt cgagaagagc cacgagaacc
     1081 tggcgccgtt ctggcggagt ctccgctgat gccatgagga tcttcggctt tccgcctttc
     1141 gaggaccgca gagacgccgg acgccggctt gcacggaagc tggaccgctt caaggacgag
     1201 cggcccgtgg tcttcgccct gccgagggga ggggtgcccg ttgggtatga ggtctcgcac
     1261 tccctcgaag cgccgctcga cgtgttcgtc tcgcgcaagc tcggggcgcc cggccagcct
     1321 gagttcggca tcggggccgt cgcgagcgga ggcgtgcgcg tcctcaacga agacgtggtc
     1381 agacggctcg gaatcccgga agaatacgtc gaatggatta ccgcgcggga gaccgcggag
     1441 gtggagcgtc gactgcgcta cttcagggga gagcgtcccg agcctgacgt cggcgggcgg
     1501 accgcgatcc tggtcgacga cggcctcgcg actggggtga cggcccgcgc cgcggtagag
     1561 gcacttcgcc agcgaagacc cgagcggctc gtgctcgccg cgcccgtgtg cgccttccag
     1621 acggcggatc tcttcagacc catcgttgac gaacttgtct gcctggagct cccttccaac
     1681 ctcggggcca tagggttctg gtacaggaac ttcgatcaaa cgtccgacga ggaggtcgtg
     1741 gaactgctcg aaagagccag gagcgaacgc ggcaccgggg agctgtgatg gaggggaata
     1801 gaaggtaatc ttcctatacc gtaccctcgg cggtcatcgt atgacggatt ggatcacgag
     1861 tacggacggc ctgtgcctgt gtagggggaa ggtcgcctct ctccgacttg agtcgagagc
     1921 ttcttcaggt tcttcgagcc tccttaccac cagcccggcg ttgacgagcg tattcacgag
     1981 ggtagcaact ttacggtggt gcttgacgat aatcccagcc aaccacgacc gctcccgcgc
     2041 accctcctcg cagtagccgt caaccggcca gtgagccctg ccctcctcat cctcgaccta
     2101 gtctacgccg ggagctttgg gtgccgtctt cattgggtgc tccacggaga agacgaacca
     2161 gccgcctgac ctcagccaac tcgccacgct ccgtaccagc gaatcgtagt cagccacgta
     2221 gtgcatcgcg agggagctgg ccaccaggtc gaagctctcc tgtggtggat ccaggtcctc
     2281 cgtggcgctc ttgaggtact cgatccgtgg gtgttggcgc tcgcttcggg ccaccccgag
     2341 catcctctcg gaggcgtcgg tggcaagcac acgcgaggcg ccttgttccg ccatgtacag
     2401 ggaaagttgc cccatgccgc acccgagctc caatacctcc aggtcctcga gtggcggcag
     2461 gcaggccctc aaggcaggtt gctcgataag ctcgttggcg ctcgccggac tctcacgcat
     2521 ccggcggtat ctttcgaaga attccgggtc gttgtagttc aatgtgacac cctccgccat
     2581 tgttccaccg caccgcgccg gcggcaacag accggtggga taggggcgtc ctccgggtac
     2641 aattagacta aggtttccag tttcgagaga ggtgagagag atgtgtggca ggtatacgct
     2701 ttctaccccg gcaggcaggc tggccgaggc tttccagctg gacgacggtg ctgtggacct
     2761 cacgccgagc tacaacgttg cgccgacgca ggaagtcgcg gcggtactcg aggaggacgg
     2821 gaagcggcgt ctcgagatgc tcaggtgggg gctcgttcct tcctgggccg acgacccgga
     2881 gatcggggcg cgcatgatca acgcccgcag cgagaccgcc ccacagaagc catcgttccg
     2941 cagagccttc cgcagcatgc ggtgcctgat ccccgccgac ggcttctacg agtggaagcg
     3001 cgagaacggc ggaaaacaac cctactactt ccacatgcac gacggacgtc cgttcgcctt
     3061 cgcagggctt tgggagcgat gggagaaggg cggtgagcta ctcacgtgcg cgatcttgac
     3121 cacgaagccc aactccgtgc tcgaagggat acacgacagg atgccggtca tccttccctc
     3181 cggtgcttat gacgcctggc tcgaccctga cgcggataga gagcaactca tcgacctgat
     3241 gatcccttac cccggcgacg acctggagac ctacccggtg agccgcttcg tcaacagccc
     3301 gcgaaacaac gacgagcgct gcatagaacc agccgcttga gcgtcgatgt cagggccgac
     3361 gtaagggagg ccctgagaga aggagcgccc gtcgtcgcgc ttgaatcgac cctgatctcc
     3421 cacggtctac cctacccaca gaacctcgag gtagccatcg aggctgaacg agccgtgcgc
     3481 acagaaggcg ccgtgccagc cacgatcggc gtcatcgatg gggtggcgaa ggtcggactg
     3541 gacgaggccg ggctggtgtt aatggcgacg gcggaaggca tcccgaagtt ctcggcgcgg
     3601 gatctcccaa ccgccgtcgc aaagggaagc cacggtgcca cgacggtcgc ggcaaccgcc
     3661 cagctcgccg cgctggcagg gattcggctc ttcgccacgg gcggtctcgg cggcgtacac
     3721 agggaggcac gtcagtcatg ggacgtctcc gccgacctcg ccgcccttgc caggacgccc
     3781 gtggcagttg tctgctcagg tgtcaagtcc atcctcgacg tgccggcgac cctggaatac
     3841 ctggagaccc tcggcgtgcc cgtcgtggga taccgtacgc gccgcttccc cggcttctac
     3901 ctgaccgact ctgggagccc actcgactgg gcggtagaga gcgcggagga ggcggcgcgt
     3961 acgatcaagg ccatgaaatc tctcggcttc gagggctcgg gtctcgtggt cgcgaacccg
     4021 ctttccgaag aagaccagct cgatcccccg ctgcacgaca gggcgctgcg tacgagcctc
     4081 gaagagctga agcgtcgcgg cgtcagcggc aaggacgtga cgcccttcct gctcgaccgc
     4141 ttcgcgaagg agacgaaggg ggagagcctg cgtgccaaca ggctcatcat tatcaacaac
     4201 gccggtctcg cggcgcgcat cgccgtggcg ctctcagccg gacgggcctg aatgaagtct
     4261 ccgcgggtcg tcgttgtcgg ggacctgcta tacgacctgc tcgcaagagt cgaggaggac
     4321 gtgaccttgg gcacggatac attcaccccc atccgggccg tcgccggtgg ttccggcgcg
     4381 aacgccgccg cctggctcgc ctcagcaggc gtcgagacgc acttcgtcgg gagggtcggc
     4441 gacgacgtct tcggcaggtt cctcgaaagg gaactggaaa gagtgggcgt gaaggcgcac
     4501 ctttccggtg acccttccat ggcgacgggc aaggtcttcg tactcgtcga tggagccgga
     4561 gaacggacca tgatcaccga ccgcggcgcc ggagaaaacc tctgctctga agacctgcca
     4621 ggatcacact tcagcagtgg acacctgcac ctctccggct acacattctc gggaggcagc
     4681 cgcagggaga cggcgagaaa ggccctgcgc ctggcgcgcg agtcagggat gaccgtctcc
     4741 gttgacccat cgtcggtccc gctgctcgcg gacgtggggc cagaacgctt cctggggtgg
     4801 accagtggcg ccgacctgtg cttcccaaac cttgcagagg gttcgctgct cgcaggtacg
     4861 gaagaccctg atctcatcgc agagaggctt ctcgaccact attccggcgt cgtcctcaaa
     4921 ctcggcgcgg agggagccat gtacgtgggc gcagagggag gacgggctag catgcccgcc
     4981 gcgtcggcgc gagtgatcga cacaacgggc gccggcgacg ccctctgcgc gggcttcctc
     5041 gccgcatggc tcgtcggagc gacgcccgca gaggctttgc ggcggggcgt cgggctcgcg
     5101 gccagcgccg tcgggcgcat cggcggtcgc ccggacgctt agaggtttcg ggtatcgagc
     5161 cagcgggtgc gctcgcgggg atcgccggta catccatagt atcttcaggt ccgtagatac
     5221 gtttgccgga gacaggaggg ttgtgttctt gagcgccgag agcccgagaa gcaagtccgg
     5281 aggaaaatga agctcgccgt cgtcgtactg gtgtctactc tcgtgctcct cgtggcggct
     5341 tgcgggcggg tctcgtcggg ccactcctcg gacgattccg ggaaacagga agcttcaggg
     5401 ataccggaac agagcaccac cgccgaagag gcaaccgtgg aggacggcgc ctcaggcgag
     5461 gcggccgagc tcgcgcgggc cgaggtgggg aagaaggaga tctccaacat gatccctgcc
     5521 gacgggaaga agcctgacgc agcgcgtccg ttgcccgagg atccacccag agggatagag
     5581 gtctatcctg ccaccacaaa ccacacagtc aggggaccca tcgaatacga ccggaagccg
     5641 ccgacaaacg gggaccatga cccgctctgg cagaactgcg gcttctacga gaagcccctc
     5701 aaggatagac acgccgtcca cagcctggac cacggtgtgg tctgggtcac cttccgtccc
     5761 gacctgccga aacggcagat caaacctcta cgttcctacg gcagggagaa ctatgtcata
     5821 gtcagccctt atcctgacca ggacgcgccc gttatcgtca cctcatggag ggtccaactc
     5881 gagttgaacg gcgcagacga cccgcgtctc aggaagttcg tgaaccagtt caggatctcc
     5941 gaaatagcgc cactctctgg caaccgctgc actctaggca tcggcgaccg gtaagggcga
     6001 ataaatcaat tcgcgggacg cggggaatgt cctcagcccg gtatgccggc gacggctgcc
     6061 tctatctcct cgtctgagtg ctccaggttg acaagttctc cggcgaggta ctgctcgtaa
     6121 gcagagaagt cgaagtggcc gtgtccgcag aggttgaaga ggatggtctt ctgttctccg
     6181 gtttccttcg cttcgagcgc gaggtccatg gcggctttga tcgcatgcgt cacctcgggt
     6241 gctggcacta tgccttccgc cctggcgaac gcgacccctg cctcgaaggt cgggttctgg
     6301 gcgtaggccc gcgcttctac gagcccctcg tgcacgagcg ccgagactat aggggagtcg
     6361 ccgtggtagc gaagtccccc ggcgtggatg ccgctcggca cgaacgagtg acccagcgtg
     6421 tacatcttca tcatcggggt cgtccccgca gtgtcgccga agtcgtaggt gtagcggccc
     6481 ttggtgagcg tcgggcaaga ggcaggctcg acggcgacga cacgcgtatt cttcccgttt
     6541 ttcaggttct cgcggatgaa ggggaaggcg acgccgccga agttcgagcc gccccctacg
     6601 cagccgatta cgatgtcggg gtactcgcct gcaagctcca tctgctgcaa cgcttcctgc
     6661 ccgatcaccg tctggtgcaa cagaacgtgg ttgaggacac tcccgagcga gtacttcgcg
     6721 tcgtcgttct tcacagcgtc ctcgatcgcc tcgcttatgg cgatgcctaa agaccccggc
     6781 gaatcaggat gctcctctaa aatgttgcgg cccgcctgcg tcaggtccgt cgggctggcg
     6841 tggacttcgg cgccgtaggt ctccatcatg atgcgcctgt agggcttctg gtcgtagctg
     6901 acgcggacca tgtagaccgt gcaatccagg cccatctgcc ggcaggcgaa agccagagac
     6961 gagccccact ggcctgcgcc cgtctccgta gtcaggcgcc tgacgccttc ctcgcggttg
     7021 tagtaagcct gcgggacggc cgtgttcggc ttgtgggagc cggtcggaga gccgccttcg
     7081 tacttgtagt agatgtgggc cggggtgtcg aggatctttt cgaggcggcg ggcgcggaag
     7141 aggggggtcg ggcgccagag cgcgtagatc tcacgcactt cttccgggat ggggacgtat
     7201 gagtctgacg ttacttcctg ttcgatgatc gcctgaggga agatcggggc gaacgcctca
     7261 ggcccgacag gttccctcgt cgcggggttc agtggcggct ccagcccgaa aggcaggtcg
     7321 gggaccagat tgtaccagct ctcggggatg gccttttcgt cgaggatgaa cttcgtctgt
     7381 tgcatctcgc acctccggcg ctcggctttc agcggtcagc cttcagctta cagcttttca
     7441 cggtatgggg taaaagggga tgaagagaat gcgcccccgc ggcggaacgg gcgcaccgag
     7501 agatgcgccc gtaaaccgga tatggatggc tattgggtca agggagcagt accactgcgt
     7561 caccaatggg acgctctacg ttgcccgggt cggaggggct gttcattagc tcttcgccga
     7621 tggtgagcga cgtcgagccg ccgccgtcga ggttgacggc ttcctcggcg cacagggcct
     7681 gcatgatgag cgcgctctcc tcgaagctcg cccccacgct gtagcctggt tgtctgccgt
     7741 ctactgccac gaggagcagg ttcccctcag gcgtgacgcc cgcgagggtg cgcgggttgc
     7801 gtctgacgcc gaagcggtag tagaactccg ggttctcggg ccagacgaac ccctcggcgt
     7861 aggcggttat gtcgggtgag ccgttgctga ggaggcgcgg cccgccgttg acgacgctgg
     7921 tcccaggggc gagtggcgcg ccgtcggcgg tgatgctcgt cgagatgttg atcgtcctcc
     7981 cgactctggc atgggcccgc agccagtcgg cggcctcgcc ggtgcctgag agcacggaac
     8041 cgttttcggg tatggggccg ccgcggctct tgcgcaactt cacgacgcgt cccgaggagt
     8101 cgagcaccgc ctcgaccccg gggcccggtt ccgtgctggt gccgaagtcg ggggtgaaca
     8161 ggatcaactc gctcgagtct gcgcaggtga agtcgtgctt tggctgctcg gtcgggctgt
     8221 ccccgccatc tccgccgcag ccgcggatca ggcccggctt gcggttgagg ccgtcgacct
     8281 cgcgtgtcgc gccgtctgat gaggtcgcgg ttatcgtgtc cgagatggcc gcgatgtctg
     8341 caccctcgcc cgagccggga gggaggatga ggctcgtgcg gccatccacg gcctcgctca
     8401 ccagagtgcc gtcgatcacc gagatgcccg cgagatcccc cggcgtgccg tcgttatcgc
     8461 cgatcacgaa gtacccaccg tttatcgcgg ccagcgagtc ggtcctgtcg gagatcgaag
     8521 tcagtgtctc cctacctggt acgatctccg tcgccagctc aggcgtgatg gtcccgttat
     8581 agagatc
//
LOCUS       DATAIR010000526         5661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_128373, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000526 DATAIR010000000
VERSION     DATAIR010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128373"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VHM69_12495"
     CDS             <1..569
                     /locus_tag="VHM69_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2741259.1"
                     /translation="ETRPFWKVKGIAILMTLGLSALILIGVLLLIAGPSIGETVAEIF
                     TLGDEFMVVWNIARWPAALLFLVATVALLFYFAPDADQPFRWITPGGFVGILLWVIAS
                     VAFNLYLSSDFNTYDKTYGSIGTVIILLLYLYISSLTILFGATLNATLVRIKEEVSGE
                     QILKAELANEKPDIIDEETVDEVQNKDG"
     gene            623..712
                     /locus_tag="VHM69_12500"
     tRNA            623..712
                     /locus_tag="VHM69_12500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Ser,seq:gct)
     gene            1309..2103
                     /locus_tag="VHM69_12505"
     CDS             1309..2103
                     /locus_tag="VHM69_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741260.1"
                     /translation="MVRQGRSIAVVGVLLIGCAVLLVAGASGVQAEASNKEDARCEGT
                     RKVNVNMPGLRTTNDLPGCPQGGLLLGTDNVDYLAGEKGDDEVRGLGGSDDIMGGPGN
                     DVIYGGSGSDGVGGYDGDDIIYGGPGNEGNGGVLVAGHEGDDVIYGGPGDDGNLVGFD
                     GKDVIYGGPGDDTELDVASADEFVGNDGQRDKLYCGEGRDTYYAEKIDYVDSSCEVKL
                     GNSVPSGSPGPGPPIADGGGPAILLPAAALLLLGSGILTYAILRRR"
     gene            2221..2652
                     /locus_tag="VHM69_12510"
     CDS             2221..2652
                     /locus_tag="VHM69_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2741261.1"
                     /translation="MSAEENKALARRELEEIFAAKGNLDAAEEIYAPHYISHQPAGDD
                     DIRGPEAIKEFAAGMREAFPDLEITIEEQIAEGDKVLTRFRTRGTHRGELWGIPPTGK
                     EVEITNMSMSRIEGGKMVEEWPAPDRLGMLQQLGVIGQPSG"
     gene            complement(2748..3212)
                     /locus_tag="VHM69_12515"
     CDS             complement(2748..3212)
                     /locus_tag="VHM69_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2741262.1"
                     /translation="MAEPDEKKRVFRLGWSTWRRAHQALAARYRKASRAAKRALDGEA
                     EVEDRAGPSTTTLCAEGAPLTDEEWQMVEPLFLPNKPPAGRPFNDHRKVLGGIMWVAG
                     TGSSWREMPEEYGKWETAYRRYELWLRQGLLQRILQALGEGALPGPATKKTN"
     gene            complement(3254..4363)
                     /locus_tag="VHM69_12520"
     CDS             complement(3254..4363)
                     /locus_tag="VHM69_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2741263.1"
                     /translation="MVAQHGTVNPAIGGWSEALEALHRRIADRFARSEARERVRRYLV
                     GLLGRVERKNGWQLAEAIGEKDPQGVQRLLNSAKWDADLVRDDLREYVVEHLGDLESG
                     VLITDETGFLKKGNKSVGVARQYTGTAGDTVDCQVGVFVAYSSKKGSAFIDRALYLPE
                     EWANDPKRRAEAGVPEEVAFANKVELAKRMLKRAFDAGVPARWVLADSFYGRSHEFRA
                     WLEKLERLAERLPDDAWSEVLPAREDSGQRRSWEWVCLGLAADPKKGMSRWLLVRRGS
                     DDPEDIAFYQAYGPEDTSVEQLVGICQERWAIESAFEEAKGEIGMDHYEVRKWGAWHR
                     YITLCLLAHAFLVVRRLAASDNEEVARKKGIPSPV"
     gene            4649..4948
                     /locus_tag="VHM69_12525"
     CDS             4649..4948
                     /locus_tag="VHM69_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741264.1"
                     /translation="MQAQRAAQRGGVGTIPRRGGRWLGALLTVAAILVIVALMALMTR
                     GMVAPADEGGVGPRDAKAGVVGSAIVYDDTGIMHASMVNEGAGAAVIHDDAGNVR"
     gene            complement(5158..>5661)
                     /locus_tag="VHM69_12530"
     CDS             complement(5158..>5661)
                     /locus_tag="VHM69_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741265.1"
                     /translation="TVDGVIDPVGEWVQPDSDHADYSFERQARSSGLVLGRKTDEGLA
                     GYWPSASGRWADMVNPMPKYVGSNTLSGDLEWNATLLEGSLEDSIPKLKDDVDGDLFM
                     HGSGEFAYALAERGLIDEYEVYLNPLVWGQGNVHVLGDRGAVRMRLDDVKRFESGVVL
                     LTYLPAT"
BASE COUNT         1093 a         1764 c         1712 g         1092 t
ORIGIN      
        1 aggagacgcg gcctttctgg aaggtgaagg gcatcgccat cctgatgacg ctcggtctct
       61 cggcgctcat cctcataggc gtgctgctac tgatcgccgg cccctccata ggcgagacgg
      121 tcgcggagat cttcacactc ggcgacgagt tcatggtggt ctggaacatc gcgcgctggc
      181 ccgcggcatt gctgttcctt gtcgcgacgg tcgccctcct gttctacttc gccccagacg
      241 ccgaccagcc gttccgctgg atcacaccgg gcgggttcgt cggcatcctg ctctgggtga
      301 tcgccagcgt cgccttcaac ctgtatctga gcagtgactt caacacgtac gacaagactt
      361 acggttccat cggcaccgtc atcatcctgc tcctctacct ctacatctcc tccctgacaa
      421 tcctcttcgg cgcgaccctc aacgccacac tcgtaaggat aaaagaggag gtctctggag
      481 agcagatcct caaagccgag ctggccaacg agaagcccga cattatcgac gaagagaccg
      541 tcgatgaggt ccagaacaag gacgggtgag ggcgtatcag gcgcgtcgaa gacgcggcac
      601 gtctttgata tactcctccc ccggaggagt ggccgagtgg ttgaaggcgg caccctgcta
      661 aggtgttata cctcttaacg gggtatcgag ggttcgaatc cctcctcctc cgctccgaaa
      721 ctcgtctatt tgcagcgtaa acctgaaaga gagcattgat ccggtagagc cctaagcctt
      781 tctctacacc aacgagtaca cctacgcaac ccgagcgggc cgtcttccgg cccgtagagc
      841 aagggtacac ttcctgcccc gtgaggttgg gagcggacct ggcctattcg agaggaaact
      901 atctagggac ctgcatagtt atccaacgcc acaatatgtt gagtcggcgc ttttcgctag
      961 aatgagcgca cctgactccg cacggctcgc ctacgacaac tttatgcagg atcgtgtatg
     1021 cagtcttcgg agaatttacc ctacagcatc acttatgcgg aacggcctgc taaacgctgc
     1081 ttgctgcggg cgtattgagg agctaaaggg ttgtaaagac gctcaaagca gggcgcaggg
     1141 caccccaaat cgaggccgct gagcacgtta gagctaagtg atgctgtaga gtatcttccc
     1201 atcacactcc ggtgaataag tcttcctgag agatgcgcca gggttcttgg tgtggcatgc
     1261 ttggaggtgc tagtagcagg ggatgcatgc aacgaagggg agggcgtcgt ggttcgccaa
     1321 ggacgcagca tagctgtggt gggggtattg ctgatcggat gcgccgttct tctcgtggct
     1381 ggcgcttcag gagtgcaggc ggaagcctcc aacaaagaag atgcccggtg cgaggggacg
     1441 cggaaggtca acgtgaacat gcccgggctc cgtaccacca acgacctacc cggctgccca
     1501 cagggcggcc tgctcttagg caccgacaac gtagactatc tggccggtga gaaaggcgat
     1561 gacgaggtac gcggccttgg cggttcagac gacatcatgg gaggccctgg caacgacgtc
     1621 atctacggcg gttcgggaag tgacggcgtg ggcggctatg atggcgatga catcatctac
     1681 ggcgggccgg gcaacgaggg gaacggcggc gttctcgtcg ctggccacga gggcgatgac
     1741 gtcatctacg gcggaccggg cgacgatggg aatttggtgg gctttgacgg caaggacgtc
     1801 atctacggcg ggccgggcga cgatacagag ttggacgtag cttcagcaga tgagtttgtt
     1861 ggcaatgatg ggcagcggga caagctctat tgcggtgaag gccgggacac atactatgct
     1921 gagaagatcg actacgtgga cagcagctgc gaggtgaagt tagggaactc ggtgcccagt
     1981 ggcagccctg gccctggccc gccgattgct gacggtggtg gtcctgccat cctcctgcct
     2041 gctgccgcgc tgcttctact ggggtcgggc atcctgacct acgcgatcct caggcgcagg
     2101 tgaacctcac gaacttctaa gaactccgtt tacgaagttc gcggaagtag cgccatcatc
     2161 caaaccttgt tacgttgggt gcgaaaacgc tgccgtgggg agcttcagga gaggagcatt
     2221 atgtccgctg aagagaacaa agctctagcg cgtagggagc ttgaggagat ctttgccgcc
     2281 aagggaaacc tcgacgctgc tgaagagatc tacgccccac actacataag ccaccaaccc
     2341 gctggcgacg acgacatacg cggtcccgaa gctatcaagg agtttgccgc tggcatgcgc
     2401 gaagccttcc ccgacctcga gatcaccata gaggagcaga tagctgaagg ggacaaggtg
     2461 ctaacccgct ttaggactcg cggtacccat cggggcgagc tctggggcat tcctcccacc
     2521 ggcaaggagg tggagataac gaacatgagc atgagccgca ttgagggagg gaagatggtc
     2581 gaggaatggc ccgcccccga ccgactgggc atgttgcagc agcttggagt cataggccag
     2641 ccgtcagggt aggcggtcga acttttctaa agggccagga gaccctagtg tcctggccct
     2701 tcctcttggc cttattcacc ctggtgcgtg gaagatgccc tacagcatca gttagtcttc
     2761 ttcgtcgccg gtcccggtaa cgctccttct cctaacgctt ggaggatgcg ttgcaagaga
     2821 ccctgcctta accacagctc gtagcgcctg taagcggtct cccacttgcc gtactcctcc
     2881 ggcatctccc gccacgacga tccggttccc gccacccaca tgatcccgcc aagcaccttg
     2941 cggtgatcgt tgaaggggcg tcccgcgggc ggcttgttag gcaagaagag aggctcgacc
     3001 atctgccact cttcgtcggt gagcggtgct ccctcggcgc acagcgtcgt ggtactcggt
     3061 ccggcacgat cctcgacctc tgcctctccg tcgagggcgc gtttggcggc ccgactcgcc
     3121 ttccggtagc gggcggcgag tgcttggtgc gcccgtctcc atgtcgacca ccctagccgg
     3181 aagaccctct tcttttcgtc cggctcggcc atggcgagca gaacgctcct tacctcaggc
     3241 accgtcagcg ggatcagacc ggactcggaa tccccttttt acgagcaact tcttcgttgt
     3301 cgctggcggc caggcgcctg acgactagga acgcatgggc caacaggcac agcgtgatgt
     3361 agcggtgcca ggcgccccac ttcctgacct cgtaatggtc catcccgatc tctccttttg
     3421 cctcttcgaa ggcggactct atggcccagc gctcctggca gatcccgacc agttgctcga
     3481 ccgaggtatc ctcggggccg taggcctggt aaaaggctat gtcttcggga tcgtccgagc
     3541 cacggcgaac caacagccac cggctcatcc ccttcttggg gtctgcggcg agccccaagc
     3601 acacccactc ccatgagcgc ctctggccgg aatcctcccg cgcaggaaga acctcagacc
     3661 aagcatcatc gggcagccgc tcggcgagcc gctctagttt ctccagccac gcccggaact
     3721 cgtgggagcg gccgtagaag gaatcggcga gcacccacct cgctgggacg cccgcatcga
     3781 atgcccgctt gagcatcctc ttggccaact cgaccttgtt cgcgaaagcc acctcctcgg
     3841 gaacgccggc ctccgccctg cgcttcgggt cattcgccca ctcctcgggc agatacagcg
     3901 cccggtcgat gaaggcggag cccttcttcg aggagtaagc cacaaacacc ccgacctggc
     3961 agtcgaccgt gtcgcccgcg gtgccggtgt actggcgggc aacgcctacg ctcttgttgc
     4021 ccttcttcaa gaagccggtc tcgtcggtga tgagcacccc gctctctaag tcgccgaggt
     4081 gctccacgac atactccctg aggtcgtcgc gtacgaggtc ggcgtcccac ttggccgagt
     4141 tcaagagcct ctggacgccc tgcgggtcct tctcgccgat ggcctcggcc agctgccagc
     4201 cgttcttgcg ctccacccgc ccgagcaagc cgaccaagta gcgcctcact cgctctctgg
     4261 cttccgaacg ggcgaagcga tcggcgatac gccggtgcag tgcttctaat gcctcgctcc
     4321 aaccgccgat ggccgggtta acggtcccat gttgggcaac catcccgctc ccttcctcac
     4381 tcggctggga tggtagcgca ctatggagct aagtgatgct gtagggatcc ttccacgtac
     4441 tccgctgaat agggagcggt gcccgtctct aggtcgccat gcgtgtacgt ccccccgagg
     4501 gtccaacaga accaagaata gtccagataa tcccagaata atatcaaaag tagcacttcg
     4561 taacgcttct cgtaacgttc ctgtatcgaa cgcccgctat gctggcctca cggtaaccca
     4621 ccgggaagca agggaaggag caaccgtgat gcaggcacaa cgagcagcac aacgaggagg
     4681 agtgggcacg atacctcgac gcggcggcag gtggcttggg gcgttgctca ccgtcgcggc
     4741 catcttggtg atagtggcgc tgatggcgct gatgacgcgg gggatggtcg cccctgcgga
     4801 tgagggtgga gtggggccac gggacgccaa ggcgggcgtc gtgggctcag ccatagtcta
     4861 tgacgacacg ggcatcatgc acgcttctat ggtcaatgag ggtgcgggcg cagctgtgat
     4921 tcacgacgac gcgggcaacg tccgataggg ctccaaggcc aaagcgttga gaggaagagg
     4981 ggccgggttg ctccggcccc tcttctcggg cgccgacttc cgaaaactcc gtcaaggcga
     5041 agttccgaaa accccctact agagtgactc cgatgtgatc gggaccgtgc taaaagccgt
     5101 gctggtgctg tccttgatcg gtacgcttgc cgcctgttcc gagaatagcc cctagcttca
     5161 agtcgccggg aggtaggtga ggaggaccac tccggattcg aaccgcttca cgtcgtcgag
     5221 cctcatccgg acggcccctc tgtcgccgag cacgtggacg ttcccctggc cccagacgag
     5281 cgggttcagg tacacctcgt actcgtcgat cagacccctc tcggcgaggg cgtacgcaaa
     5341 ctcgccgctc ccgtgcatga acaggtcacc gtcgacgtcg tccttcagct tcgggatcga
     5401 gtcctcgagg gacccctcga gcagggtcgc gttccactcg agatcgccgg acagcgtgtt
     5461 ggacccgacg tacttcggca tcgggttgac catgtccgcc catctgcccg acgcgctcgg
     5521 ccagtaaccc gcaagaccct cgtcggtctt gcggcccagc accagcccgc tggatctcgc
     5581 ctgacgctcg aacgagtagt cggcgtggtc tgagtccggc tgcacccact cgccgaccgg
     5641 gtctatgacg ccgtcgacgg t
//
LOCUS       DATAIR010000527         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_405152, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000527 DATAIR010000000
VERSION     DATAIR010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405152"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..750)
                     /gene="uppS"
                     /locus_tag="VHM69_12535"
     CDS             complement(67..750)
                     /gene="uppS"
                     /locus_tag="VHM69_12535"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2741266.1"
                     /translation="MIPHHVAIIMDGNGRWAQRRWLPRAAGHRAGVSVLTPLLEIAGQ
                     AGVKTLTLYAFSTENWGRPETEVKTLMKLFLETARAKVPELNERGARLRFLGRREDLP
                     ADVLQAMDEAEELTAGNDSLDVYIALNYGGRSEIVDATRRIVSAGLSPDEVDEGTFAR
                     YLYAPEVPEVDLVIRTSGELRVSNFLLWQIAYAEFYVTDTLWPDFSTQEFLHAIESFA
                     SRSRRRGGV"
     gene            complement(826..1386)
                     /gene="frr"
                     /locus_tag="VHM69_12540"
     CDS             complement(826..1386)
                     /gene="frr"
                     /locus_tag="VHM69_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2741267.1"
                     /translation="MSDVIDLTAAERRMNGAVEAVKSNFSTIRTGRANPSLLDRIEVN
                     AYGSRMPLRSVANVGAPEARLLTVTPFDPNSLKDIERAIMDADIGLNPQNDGRILRLP
                     IPELTEERRRELVRMARSMAEEGRVSVRNVRRDEMRDLHELRKEGEISQDDEHRAEEE
                     LQKLTDSYVKRVEAALADKEAELSEV"
     gene            complement(1388..2182)
                     /gene="pyrH"
                     /locus_tag="VHM69_12545"
     CDS             complement(1388..2182)
                     /gene="pyrH"
                     /locus_tag="VHM69_12545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2741268.1"
                     /translation="MEESARYSGETNGAGLSELGPVKRVVLKISGESLAGESGYGIDP
                     GSLDSTAKGVFEAVEAGAELAVVVGGGNIFRGSHVADELGIEGATADYMAMLGTVINA
                     MALQAALEGRGVPTRVQSAIDIQEVAEPYIRRRAIRHLEKGRVVIFASGTGNPFFTTD
                     TGAALRALEINADALLMAKNRVDGVYDKDPRRHPDAVRLSRLNYMDLLANNLSVMDHT
                     AATLCSGENLPIVVFDILKEGNLRRILQGERVGSLVWGAANGGGGG"
BASE COUNT          361 a          732 c          683 g          441 t
ORIGIN      
        1 atgcttgcga gcgcgatcgg cgccagaacc agggctgtgg ctgccctcca ccgtagcacg
       61 gtatccctag acgccgcccc ggcgtctgga ccgggacgcg aaggactcga tggcgtgcag
      121 gaattcctgc gtggagaaat caggccacag cgtgtcggtc acgtagaact ccgcgtaggc
      181 aatctgccac agaaggaaat tcgagacacg aagctcgccg ctggtccgga tcaccaggtc
      241 aacctctggc acttcggggg cgtagaggta gcgtgcgaag gtcccctcgt ctacttcgtc
      301 aggcgagaga ccggctgaga ctatcctgcg ggtagcgtca acaatctcag agcgtccgcc
      361 gtagttgagc gcgatgtaca cgtcgaggct gtcgttccca gccgtcagct cctccgcctc
      421 gtccatggct tggagcacgt cggcgggcag atcctccctt cttccgagga aacggagcct
      481 ggccccacgc tcattcaact cgggcacctt cgcacgcgcc gtctccagga agagcttcat
      541 gagggtcttg acctctgtct cggggcggcc ccagttctct gtggagaacg cgtacagggt
      601 cagggtcttc accccggcct ggcccgcgat ctcgagaaga ggcgtcagca cggagacacc
      661 tgcccgatgg cctgcggcgc gcggaagcca ccgtctctgc gcccagcgcc cgttaccatc
      721 catgatgatg gcgacgtgat gcgggatcag ctcagggtcg aggcggttga ggacgcgctg
      781 ctcgggggag tctgggggga gtcgtttctc gacgggttcc agcagttaga cctccgagag
      841 ctcggcttcc ttgtcggcaa gggcagcctc caccctcttc acgtagctgt cggtgagctt
      901 ctgaagctct tcctcggccc tgtgctcgtc gtcctgggag atctcccctt ccttgcgaag
      961 ctcgtggagg tccctcatct cgtcgcggcg cacgttcctc accgagaccc ttccctcctc
     1021 ggccatgctc ctcgccatgc gtaccagctc gcggcgacgt tcctcggtga gctccggtat
     1081 cggtagcctg agtatcctgc cgtcgttctg cgggttgaga ccgatatctg catccattat
     1141 ggcgcgctcg atatctttca acgagttcgg gtcgaagggg gttacggtca ggagtctcgc
     1201 ctcgggcgcg ccgacgttgg cgacgcttcg caggggcatc ctcgatccgt aggcgttgac
     1261 ctcgatcctg tccaggagcg aagggttcgc cctgcccgtg cgaatggtcg agaagttact
     1321 cttcacggcc tcgacggccc cgttcatcct ccgctcggcc gctgtgagat ctataacgtc
     1381 cgacatctca gcctcctccg cctccgttag cggcgcccca caccagcgac cccaccctct
     1441 ctccctgcaa gatgcggcgc aggttgcctt ccttaagtat atcgaaaacg actatgggca
     1501 ggttctcgcc gctgcacaac gtggccgcgg tgtggtccat gacggaaagg ttgttggcga
     1561 gcaagtccat gtagttgagg cgcgagaggc gcacggcgtc ggggtgccgc ctcgggtcct
     1621 tgtcgtagac cccgtccacc ctgtttttcg ccatgagcaa ggcatcggcg ttgatctcca
     1681 gggcacgcag cgccgccccg gtatctgtcg tgaagaacgg gttacccgtc cccgaggcga
     1741 agatgacaac gcgtcccttt tcgaggtgcc ttatggcacg cctgcggatg tagggttctg
     1801 cgacctcctg gatgtcgatg gctgattgga ccctggtcgg gacgccgcgc ccttcgaggg
     1861 cggcctgcaa cgccatcgca tttatgaccg taccgagcat cgccatgtag tcggccgtgg
     1921 ccccttcgat accgagctcg tcggcgacgt gcgaaccgcg gaagatattg ccgccgccaa
     1981 cgaccacagc aagctcggcc ccggcctcga ccgcttcgaa gacgcccttt gccgtcgagt
     2041 cgaggctgcc agggtcgatc ccgtagccgc tctcgccggc gaggctctca cccgagatct
     2101 tgagtacgac gcgctttacg gggccgagct ccgagagccc ggccccattg gtctcgcccg
     2161 aatatctggc ggactcttcc acgcctctac tagagctcgt agtggacgaa gcgccgg
//
LOCUS       DATAIR010000528         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_200855, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000528 DATAIR010000000
VERSION     DATAIR010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200855"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VHM69_12550"
     CDS             <1..304
                     /locus_tag="VHM69_12550"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX2741269.1"
                     /translation="SFGSFDSFKEAFGAAAAPGALFGSGWCWLVGNSDGSVSIETTPN
                     QDTPLMEGRTPVIGLDVWEHAYYLKYQNRRPEYIEAWWSVVNWDEAERRYQAAKSA"
     gene            483..1859
                     /locus_tag="VHM69_12555"
     CDS             483..1859
                     /locus_tag="VHM69_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2741270.1"
                     /translation="MRLVIVGGVAAGTKAASRARRLDPEMEITVYQDEPEPSISECGL
                     PYFLSGVVEQREKLIVRTPERFARDGIEVLVRHRVEKIDPTARTLSVLDLKTGETFDD
                     HYDRLIIATGARAVLPPIPGADLEGVFKLRFLTDADEVGRYIEEISPKKATIVGGGYI
                     GLEVAENLCRLGMEVSLIEGEDRVALAYGPEVSDRVETELEAHGVGVYTGEVVDEFTG
                     EGRIRGVKVGGKELESDLVIVGVGIRPNAELAAEAGVEIGSTGAIKVDRHMKTSLPDV
                     WAAGDCAQTTNLVSGRPAWVPLGDTANQMGRVAGTNAATGEDTLEFPGVLGTGIFKVF
                     DLGVGKTGLSEREATEAGFEVVSQGLEANDRASYYPGARKVFVKLIVDRATGRLLGAE
                     AAGAGAAKLTDICAAALWGGLSYPDLVNIDLAYSPPFGPTLSPVIQAATILSGKFDRA
                     REGVRASE"
     gene            complement(2119..3669)
                     /gene="pruA"
                     /locus_tag="VHM69_12560"
     CDS             complement(2119..3669)
                     /gene="pruA"
                     /locus_tag="VHM69_12560"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX2741271.1"
                     /translation="MGLIPYKNEVYLDFSDEKNVESMRAALKDVGSRLDRSYPAIIGG
                     KRVETDGEIVSVNPANPSQVVGRTARATEREADIALDMATAAFEYWSRTSPEARARIL
                     LRASAIMKRRRFELLAWEAYEGGKPWAEADAQVAEAIDFLEYYAREMLRLKDGVEIYA
                     IPGEESRYTYQPMGVGVIIAPWNFPTAILTGMSSAAIVTGNTIIMKPSEFTPVIGAVI
                     AEIFEEAGLPEGVLNYLPGYGSEVGDYLVKDARTRFISFTGSMKTGLRINELAAKQIE
                     NQRWVKRVIAEMGGKDAMVIDDSADLDSAAADIVKSAYGYSGQKCSAASRAILHADIY
                     DEVLDKVVERAKALKIGVPESADVNVGPVISEPQFEKVAGYVDVGKQEGERVLGEDPG
                     DPDNGYFVSPTIFEVDRDARVAQEEIFGPVLAVIKARDFDDALHVANDSPYGLTGGVY
                     SKNRDHLERARQEFQAGNVYFNRGITGALVGVQPFGGFGMSGTDSKAGGPDYLPLHML
                     ARTVVERF"
BASE COUNT          689 a         1143 c         1200 g          683 t
ORIGIN      
        1 atcgtttgga tcgtttgatt cgttcaagga agcgtttggt gcggcggcgg caccaggagc
       61 gttgtttggt agtggatggt gctggctagt agggaactct gatggatcgg tgtcgataga
      121 gacgacgccg aatcaggaca cgcccttgat ggagggaagg acaccagtaa tagggttgga
      181 tgtgtgggag cacgcatact atctgaagta ccagaacagg cgtccggagt acatagaggc
      241 atggtggtct gttgtgaact gggacgaagc tgagagaagg tatcaggctg ccaagagcgc
      301 ctagagcctc ttgaggcgag agcccagagc gcttcaacaa agtgcgggag cccccggaat
      361 tgacttcggg ggcttcttca tgcaccgggt agtgtgtacg gtcttaagaa cagagacaga
      421 cttcgcgttc aggtcaaagc cggtagactg aggtgctggc gagagtggaa ggatcccaat
      481 atatgcggct tgtgatcgta ggcggcgtgg cggctgggac gaaggctgcg tcgagggccc
      541 gcaggctgga cccggagatg gagatcacgg tctaccagga tgaaccggag ccctccataa
      601 gcgagtgcgg cctgccctac ttcctctcgg gcgtggtcga acagcgcgag aagctcatcg
      661 tccgcactcc cgagcggttc gcgcgggacg gcatcgaggt cctcgtccgc cacagggtag
      721 agaagatcga ccccactgcc agaacgctct ccgtgctcga cctgaagacg ggcgagacct
      781 tcgacgacca ctacgacagg ctcatcatcg caaccggcgc gcgggccgtc ctccccccca
      841 tacccggcgc ggatctcgaa ggcgtattca agctccgctt ccttaccgat gccgacgagg
      901 taggtagata tatcgaggag atctccccga agaaggcgac catcgtcggc ggggggtaca
      961 tcgggctgga ggtggctgag aacctttgcc ggctcgggat ggaggtctcc ctgatcgaag
     1021 gcgaagacag ggtcgccctc gcctacggcc ccgaggtctc cgatagggtt gagaccgagc
     1081 tggaagcgca cggcgtcggc gtctacacgg gcgaggtggt cgatgagttc accggcgaag
     1141 ggcgcatcag aggcgtgaag gtcggtggca aggaactgga gtcggacctg gtgatcgtgg
     1201 gcgtcgggat caggcctaac gccgagctgg ccgcggaagc gggggtggag atagggagca
     1261 ccggagccat caaggtagac aggcacatga agaccagcct ccccgacgtc tgggccgccg
     1321 gggactgcgc gcagacgacg aacctggtca gcggaagacc ggcctgggtc cccctcggcg
     1381 acacggcgaa ccagatgggc cgcgtcgccg gcaccaacgc ggccaccgga gaagacactc
     1441 tggagtttcc cggcgtcctc ggcactggca tcttcaaggt cttcgacctc ggcgttggca
     1501 agacgggcct ctccgaaagg gaggccacgg aagcaggctt cgaggtcgtg agccagggac
     1561 tcgaggccaa cgaccgcgca tcctactacc ctggcgcgcg gaaggtcttc gtcaagctca
     1621 tcgtggaccg cgcgacgggc cgtctgctcg gcgcggaagc agccggagcg ggcgcggcca
     1681 agctcaccga catctgtgcc gcagcactct ggggcgggct ctcgtacccc gacctcgtca
     1741 acatagacct cgcctactct ccacccttcg gcccgacgct gagtcccgtc atacaggccg
     1801 ccactatcct ctcgggcaag ttcgatcgtg ccagagaggg tgtcagggcc tctgaataac
     1861 agcccgtcgg catttcagca cgctacgacc ttcgggctcc gctcttcagc cggtcagcca
     1921 gggtgtcggt ggcgccgccc gccgacacca gcgaatccag gagcccacaa ggagacccac
     1981 acaaagctct tacccgagta attagccggc taacaatctg ggtaaaacag gctgaattgc
     2041 tgacaagccg cgaagcggcg tgctgaagtg ctgaccagcg cggggtgggg aaggtgttgc
     2101 cccttcccca tcctgctttc agaacctctc gacgactgtc cttgcgagca tatgcagtgg
     2161 caggtagtcc gggccgccgg ctttggagtc ggtgccgctc atgccgaagc cgccgaacgg
     2221 ctggacgccg acgagggctc ccgtgatgcc gcggttgaag tagacgttgc cggcttgaaa
     2281 ctcctggcgg gcgcgctcca ggtggtcgcg gttcttcgag tagacgccgc ctgtgagacc
     2341 atacgggctg tcgttggcga cgtggagggc atcgtcgaag tctcgggcct ttatgacggc
     2401 gaggacggga ccgaagatct cctcctgggc gaccctggcg tcacgatcga cctcgaagat
     2461 ggtcgggctt acgaagtagc cgttgtccgg atcgccggga tcttcaccca gaacccgctc
     2521 gccctcctgc ttgcccacgt cgacgtagcc ggcgaccttc tcgaactgcg gctcgcttat
     2581 gacgggaccg acgttcacgt ccgcgctctc tggaacgccg atcttgaggg ccttcgccct
     2641 ctcgacgacc ttatcgagca cctcgtcata gatgtcggcg tgcaggatgg cgcggctcgc
     2701 cgcgctgcat ttctggcccg agtagccgta ggcgctcttg acgatgtcag cagcagccga
     2761 gtcgaggtcc gctgaatcgt ctatgaccat ggcgtccttg ccacccatct cggctatgac
     2821 cctcttgacc cagcgctggt tctctatctg cttcgccgca agctcgttta tcctcagacc
     2881 cgtcttcatc gagcctgtga aggagataaa ccgcgtgcga gcgtctttga cgaggtagtc
     2941 accgacctcg gacccgtagc ctgggaggta gttcaggacg ccctcgggca gcccagcctc
     3001 ctcgaagatc tcggcgatca cggcccctat aacaggcgtg aactcggatg gcttcatgat
     3061 gatggtgttg cccgtgacga tggccgcgct ggacatcccc gtaaggatgg cggtcgggaa
     3121 gttccatggg gcgatgatga ccccgacgcc catcggctgg taggtatagc ggctctcctc
     3181 gccggggatc gcgtagatct ccaccccgtc ctttagccgc agcatctcgc gggcgtagta
     3241 ctccaagaag tctattgcct cggccacctg agcgtcggcc tctgcccagg gcttgccgcc
     3301 ctcgtaggcc tcccaggcga gaagctcgaa cctcctgcgc ttcatgatcg ccgaagcccg
     3361 caacagtatg cgcgcccgcg cctcgggcga agtgcggctc caatactcga acgctgcagt
     3421 cgccatatca agcgctatgt ctgcctcgcg ctccgtggcg cgggcggtgc ggcctacgac
     3481 ctgcgagggg ttcgcggggt tgacggagac gatctcgccg tccgtctcaa cgcgcttgcc
     3541 gccgatgatc gcagggtagc tccggtccag ccgggagccg acatctttca gggcggcccg
     3601 catcgactcg acgttcttct cgtcagagaa gtccagatat acttcgttct tgtacggaat
     3661 cagtcccatg atgctcctat ctccttatcg tgccggttat gcccccgatc acgtc
//
LOCUS       DATAIR010000529         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_312811, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000529 DATAIR010000000
VERSION     DATAIR010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312811"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..524
                     /locus_tag="VHM69_12565"
     CDS             144..524
                     /locus_tag="VHM69_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741272.1"
                     /translation="MARTVLAAVEDLLFKSKISETASTLGVEAAFPRNPRKLLEALRE
                     SPPDLLVLDLNSARFEPLSLLHTVKSDERTQNVSTVGFLSHVQKDLAVAAREAGCDRV
                     IARSAFTKDLPRILAGRTPEAGVG"
     gene            517..1071
                     /locus_tag="VHM69_12570"
     CDS             517..1071
                     /locus_tag="VHM69_12570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2741273.1"
                     /translation="MAEGDGWTRLGSERLIETPYFSLRSDRLRLPDGGIKDPYYVIER
                     PDAAIIFPLTAGNEVVLVRQYRPPLEGMELGLPAGLVEAGENPEAAARRELSEETGYA
                     GGAWEPLGSVASSPSLKDNRAYLFLARGVEETSAPDPDEHELVETVRVPIEDIRDLVR
                     EGEIVSSSGVAAIMLALERLREAP"
     gene            1068..1640
                     /locus_tag="VHM69_12575"
     CDS             1068..1640
                     /locus_tag="VHM69_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741274.1"
                     /translation="MSEIAPGYSILDLVAGDEEAVRQAAILLVEGFKEDWPDAWPTME
                     EALREVREAIGEGRICRVAVDEDGEVLGWIGGIPSYEGKVWELHPLVVRHDLQRRGIG
                     RALVTDLEARVRERGAMTLWLGSDDETGMTTLAGVDLYPDVLEHLARIENLRGHPYEF
                     YQRLGFSIVGAMPDANGFGKPDIYMAKRCS"
     gene            complement(1703..2047)
                     /gene="erpA"
                     /locus_tag="VHM69_12580"
     CDS             complement(1703..2047)
                     /gene="erpA"
                     /locus_tag="VHM69_12580"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX2741275.1"
                     /translation="MEQKQETIVNITDNAADKVRTLLAQEGEEDLALRIGVRPGGCSG
                     FQYNIYFDDEIADDDEVFETKGVRVLIDAMSVPYIMGSEFDYVDGLMGAGFAVNNPNV
                     QGGCGCGSSFTC"
     gene            complement(2112..>3665)
                     /locus_tag="VHM69_12585"
     CDS             complement(2112..>3665)
                     /locus_tag="VHM69_12585"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX2741276.1"
                     /translation="TGMIDWTRIGTGGTSRLDVQADVGDYLTAGRDAELLRIQSSEPL
                     LWRGGTMDYFDGVRWSDTTQPGAYDGEEIAADVKTSPVLQRVQVLNANTDLIFGGYKI
                     VRPSAYLLTTQNSDGSWSTSEPFEEGDTYEVLSEIPQPTTAQLRGAGSDYSPAIEQRF
                     LQLPDQTPPVVDETAKMIMRKYDPETPYDEARAVERYLIYDGGFVYNLDVSYRRADKA
                     IEEFLGDGKEGFCTQFSTSMALLLRDMGVPSRVVYGATTGEDVGQGEYLVTGSNMHTW
                     VEVYFPGVGWYPFNPTPGFSMPAAMEANAPRPAAPLAGQFQGPENNPAQRRQIQGREQ
                     QTPQEQNQNSQDDTATGRSGNNGAVAWPLLALFSVLLVVAVPLFKRALVLRGRPEDLY
                     RDLTGRLRDVLPPGKSIVADSHALTPTERVLLLAGAADVEEGPMREFALAYTDHLYSA
                     GGGTDHVSSAYQGAMRAYERLPRWRRALGAVNPSSLLIRWRRYVSLQRARLGKALRGR
                     VRRVFRWRR"
BASE COUNT          664 a         1189 c         1152 g          660 t
ORIGIN      
        1 gcgtgaagtt ggaaagtgtc tctgtggtgc gccgtagccc ggcgcgaagc tccttcaatc
       61 ttttcacccg gataggatac cgcacagcct gcgggtagaa tgtgcgagct tacccaacgc
      121 tagaacacgg aggcgaacag cacgtggcga gaacagtact tgcggccgta gaagacctat
      181 tgttcaagag caagatctcc gagaccgctt caacgctcgg cgtcgaggcg gcgttcccgc
      241 gaaacccccg taaattactc gaagccctgc gcgagtcccc acccgacctg ctcgtcctcg
      301 acctgaactc ggcccgcttc gagccgctct cgctgctgca caccgtgaag tccgacgaaa
      361 ggacccagaa cgtgtctacc gtcggcttcc tctcccacgt ccagaaggac ctcgccgtcg
      421 ccgcgcggga ggcaggctgc gaccgcgtaa tcgcccgcag cgccttcacg aaagacctgc
      481 cgcggatact cgccggtcgc acccctgagg ccggcgttgg ctgagggcga cggctggacg
      541 cgcctgggta gcgagaggct catcgagacg ccgtacttct ccttacgttc agaccggttg
      601 cggctgcctg acggggggat caaagacccc tactacgtaa tcgagcgccc cgacgccgcg
      661 ataatcttcc cgctcacagc cggtaacgag gtcgtcctcg tccgccagta ccgcccgccg
      721 cttgagggga tggagctcgg ccttccggca ggcctcgtcg aagcaggtga gaatcctgag
      781 gcggccgccc gccgagagct ctcggaagag acaggctacg ccggcggcgc gtgggaacct
      841 ctgggctccg ttgcctcctc ccccagcctc aaggacaaca gggcctacct cttcctcgcc
      901 cgcggcgtcg aggagacctc cgcgccagac cccgacgaac acgagctcgt cgaaacggtc
      961 cgcgtgccaa tagaggatat ccgcgacctc gtcagagaag gcgaaatagt cagctcaagc
     1021 ggcgtcgcgg ccatcatgct cgccctggag cggcttcggg aagctccgtg agcgagatag
     1081 cgccgggcta ctcgatcctg gacctcgtcg caggtgacga agaagcagtc aggcaggcgg
     1141 cgatcctcct ggtcgaaggt ttcaaggaag actggcccga tgcctggccg actatggaag
     1201 aagccctccg ggaggtacga gaagccattg gagaaggtcg gatctgccgg gtagccgtag
     1261 acgaagacgg cgaagtgctc ggctggatcg gcggcatccc ttcctacgag ggcaaggtgt
     1321 gggagctgca ccctttagta gtgcgccacg accttcaacg caggggtata ggccgcgccc
     1381 tcgtcaccga tctggaggca cgtgtcaggg agcgcggcgc catgacgctc tggctcggca
     1441 gcgatgacga aacaggcatg acgaccctcg cgggcgtgga cctctacccg gatgtcctgg
     1501 agcacctcgc gcggatagag aacctgcgcg ggcacccata cgagttctac cagaggctgg
     1561 gtttctcgat agtcggcgcg atgcccgacg ccaacggatt cggaaaaccg gatatctaca
     1621 tggccaagcg ctgcagttga ggcaaagtaa aagcccggag cttattgctc cgggctcgtt
     1681 accttctaaa cgagttagcg gtctagcagg taaaagaact gccgcagccg cagccgccct
     1741 gcacgttggg gttattgacg gcgaagcccg cccccatgag gccgtccacg tagtcgaact
     1801 cggagcccat gatgtaagga acactcatcg cgtcgataag gaccctgacg cccttggtct
     1861 cgaagacctc gtcgtcgtcg gcgatctcgt cgtcgaagta gatgttgtac tgaaacccgg
     1921 agcagccgcc tggcctgacg ccgatacgca gcgcgaggtc ctcctcgcct tcctgggcca
     1981 ggagagttct tactttgtcg gcggcgttat cggtgatgtt gacaattgtc tcttgcttct
     2041 gctccatcag cacctcccgt gctgtagtcg gctcttggtt agcgaaatta ttacaccgca
     2101 aaccgtatag ttcaacgccg ccatcggaag acccggcgaa ctcggccacg cagcgccttg
     2161 ccgagtcggg ctctctggag ggagacgtac ctcctccacc ggatgagcag cgaggaaggg
     2221 ttcacggccc cgagcgccct ccgccagcga ggcagacgct cgtaggccct catggccccc
     2281 tggtaagcgg aggatacgtg atccgtccca ccccccgcgg aatacaggtg atccgtgtat
     2341 gccagggcga actcccgcat cggaccttcc tccacgtccg ctgcccctgc gaggagcagt
     2401 acccgctcgg tcggggtcag cgcgtgagaa tcggccacga tgctcttgcc tggcggcagc
     2461 acgtcacgca gacggcccgt gaggtccctg tagaggtcct cgggcctgcc gcggaggacg
     2521 agcgccctct tgaacagcgg tacggccacc acgagcagca cggagaaaag cgcgaggaga
     2581 ggccacgcca cggcaccgtt gttcccggaa cggcctgtgg cggtatcgtc ctgggaattc
     2641 tggttctgct cctggggtgt ctgctgctcc cttccctgaa tctgcctgcg ctgggccgga
     2701 ttgttctcgg ggccctggaa ttgaccggcg agcggagccg ctggtcgtgg ggcgttcgcc
     2761 tccatcgcgg cgggcatgga gaagcccggg gtcgggttga aggggtacca gcccacgccg
     2821 gggaagtaga cctccaccca ggtgtgcatg ttgctccccg taaccaggta ctctccctgg
     2881 cccacgtctt ctccagtcgt ggccccgtag acgacccgcg agggcacgcc catatcacgc
     2941 aggaggaggg ccatcgaggt cgagaactgg gtacagaagc cctccttgcc gtcaccgagg
     3001 aactcctcta tcgccttatc ggcccggcgg tagctcacgt ccaggttgta gacgaagccg
     3061 ccgtcgtaga tcaggtaccg ctcgaccgcg cgggcctcgt cgtagggcgt ctcggggtcg
     3121 tatttgcgca tgatcatctt cgccgtctcg tcgactacgg gaggcgtctg gtcggggagt
     3181 tggaggaacc tctgctctat ggccggcgag taatcgctgc ctgcgcccct gagttgtgcc
     3241 gtggtcggct gcggtatctc cgagagcacc tcatacgtat caccctcttc gaagggttcg
     3301 ctcgtcgacc acgagccgtc cgagttctgg gtcgtcaaga ggtacgcgga gggacgcacg
     3361 atcttgtacc ccccgaagat gaggtcagtg ttggcgttga ggacctgcac tctctgcagc
     3421 acggggctgg tcttgacgtc ggcggcgatc tcctcgccgt catatgctcc cggctgcgtg
     3481 gtatcagacc atctgacgcc gtcgaagtaa tccatggttc ccccgcgcca caggagcggc
     3541 tcggagctct gtatccgcag gagctcggcg tccctccccg ccgtgaggta atctccgacg
     3601 tcggcctgca cgtcgagcct ggaggtgccg cccgtcccga tgcgcgtcca atctatcatg
     3661 cccgt
//
LOCUS       DATAIR010000530         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_128725, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000530 DATAIR010000000
VERSION     DATAIR010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128725"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHM69_12590"
     CDS             complement(<1..506)
                     /locus_tag="VHM69_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2741277.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPLTTLMLA
                     GIREMLVISTPEDLSRFEDLLGDGSQWGVSFEYAVQPKPEGLAQAFIIGREFVGSESV
                     SLVLGDNIFFGHGFSELLERSAEREGGATVFGYYVRDPERYGVVEFDTDGTVLSIEEK
                     PESPRSHYA"
     gene            complement(503..1585)
                     /gene="rfbB"
                     /locus_tag="VHM69_12595"
     CDS             complement(503..1585)
                     /gene="rfbB"
                     /locus_tag="VHM69_12595"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX2741278.1"
                     /translation="MQEEQREKQTILVTGGAGFIGGNFVLDTVGDFEVVNLDALTYAG
                     NPDTLASISENPDHVFINGRIGDRELVDRLLAEYRPTAVVNFAAETHVDRSIDGPKAF
                     IQTNVVETHELLEAALSYWSALEGEDHDSFRFLHVSTDEVYGTLGPTGYFTEESPFRP
                     NSPYAASKASSDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEKLVPLMILTALRGELL
                     PVYGDGQQIRDWLYVKDHCRAILTVLERGRSGETYNVGGHNERTNLEVVRTICALLDE
                     LAPDSPHAPHDSLITFVTDRPGHDRRYAIDAGKIERELGWTPEETLETGMEKTVRWYL
                     ENRGWCEAVLSRGYRGERLGIRGGLA"
     gene            1869..>2440
                     /gene="thrC"
                     /locus_tag="VHM69_12600"
     CDS             1869..>2440
                     /gene="thrC"
                     /locus_tag="VHM69_12600"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2741279.1"
                     /translation="MSVQARVFRQGVIRRYREWLPEIPDDAIATIGEGETPLIEASRI
                     SERVGARLFLKFEGMNPTASFKDRGMTVAMSRAAATGSRACVCASTGNTAASAAAYAA
                     RAGMECFVVVPAGKIALGKAVQVLAHGARIVQIEGNFDAALKLSKRIAEELGDVTLVN
                     SVNPDRIEGQKTAACEVAEALGHPPDALALP"
BASE COUNT          430 a          758 c          781 g          471 t
ORIGIN      
        1 gcgtagtgcg agcgtgggct ctcgggcttc tcctcgatgc tgaggaccgt tccatccgtg
       61 tcgaactcga ccacgccgta gcgctcgggg tcacggacgt agtagccgaa gacggtcgcc
      121 ccgccctccc tttccgccga ccgctccagg agctcggaga agccgtgtcc gaagaagatg
      181 ttgtccccga ggacgagcga gacgctctcg gacccgacga actcccgccc tatgatgaaa
      241 gcctgggcga gcccctccgg cttcggctgc acggcgtact cgaagctgac gccccactgc
      301 gagccgtcgc ctagaaggtc ctcgaagcga gagaggtcct ccggggtgga gatcacgagc
      361 atctccctga tgcccgcgag catcaacgtg gtcaaggggt agtagatcat gggcttgtcg
      421 tagacgggga ggagctgctt gctcaccgcc cttgtaagcg gatacagcct cgtccccgac
      481 cccccggcga gcacgatgcc cttcatgcca gccctccgcg tatgccaagc cgctcgcccc
      541 tgtagccacg cgagagcacc gcttcgcacc acccgcggtt ctcgaggtac cagcgcaccg
      601 tcttctccat gcccgtctcc agcgtttcct cgggcgtcca accgagctcg cgttctatct
      661 tccccgcgtc tatggcgtag cgcctgtcgt gacccggcct gtccgtgacg aaggtgatga
      721 gagaatcgtg cggcgcgtgc ggcgaatccg gtgccaactc gtccagcagg gcacagatcg
      781 tcctgacgac ctcgaggttc gtccgctcgt tgtggccgcc gacgttgtac gtctcgccag
      841 atcgcccgcg ttccaggacc gtcagaatag ccctacaatg gtccttcacg tagagccagt
      901 cacggatctg ctgcccgtcg ccgtagacgg gcaagagttc gccgcgtagg gcggtcagga
      961 tcatcaacgg tacgagcttc tcggggaagt ggtatggccc gtaattgttg gagcagtttg
     1021 tggtgagcgt tggtagtccg taggtgtgat ggtatgcccg cacgagatgg tcagaggatg
     1081 ccttcgaggc tgcgtaaggc gagttcggcc tgaaagggct ctcctcggtg aaatatcccg
     1141 ttggcccgag cgtcccgtac acctcgtccg tggagacgtg caggaagcgg aaggagtcgt
     1201 gatcctcccc ctcgagcgcg ctccagtacg agagcgccgc ctcgaggagc tcgtgggtct
     1261 cgacgacgtt ggtctgtatg aaggctttcg gaccgtctat ggaacggtcc acatgggtct
     1321 cggcggcgaa gttgacgacg gcagtcggtc gatactcggc gagaaggcgg tccaccagtt
     1381 ccctatctcc gatcctcccg tttatgaaga cgtggtcagg gttctccgag atggaggcga
     1441 gggtgtcagg gttgccggcg taggtcaggg cgtcgaggtt gaccacctcg aagtccccga
     1501 ccgtatcgag gacgaagttg ccacctataa aaccggcgcc gccggtgacg aggatggttt
     1561 gtttctctcg ctgctcttcc tgcaaagcac tcctctttga ggtatcaggt atcaggctac
     1621 aggcatcaga acctgaaacc tgacgcctta ttgctatagc ctactaaacg gagcgtgggg
     1681 tcgccacgtt cttgaaactc tgcattggtg cgtgatactt ttcttacgct gtggtctttg
     1741 gcttctatac tctcgttcgc ggcgcgcgaa ctacgggcgc agggggatgt atcccctgtt
     1801 gcgtttgtta gatcccacgg tcctccacaa aggacaggct cgacctccga tgaaaggcca
     1861 ggttcatttt gagcgttcag gcgcgggtgt tcagacaggg cgttatccgc aggtaccgtg
     1921 agtggttgcc cgagataccg gatgatgcca tagccaccat aggcgagggc gagacgcccc
     1981 tcatcgaagc ctccaggatc tcggagcggg tcggcgcccg cctgttcttg aagttcgagg
     2041 ggatgaaccc gacggcctcg ttcaaggacc gcggcatgac ggtggccatg agcagggcgg
     2101 ccgcaacggg gagccgcgcc tgcgtctgcg cctcgacggg gaacaccgcg gcctcggcag
     2161 cggcctacgc agcgcgtgct ggcatggagt gcttcgtcgt cgtgccagca ggcaagatcg
     2221 cattgggcaa ggccgtgcag gttctcgcgc acggggcgcg gatagtccag atcgagggca
     2281 acttcgacgc ggccctcaag ctctcgaagc ggatcgcaga ggagctgggc gacgtcaccc
     2341 tggtgaactc cgtgaacccc gaccgcatcg agggccagaa gacggcggcc tgcgaggtcg
     2401 ccgaagccct cggccaccca cctgacgccc tggccctgcc
//
LOCUS       DATAIR010000531         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_405351, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000531 DATAIR010000000
VERSION     DATAIR010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405351"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VHM69_12605"
     CDS             complement(<1..1711)
                     /locus_tag="VHM69_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2741280.1"
                     /translation="MERQTEYQFFGPNLGYVLELYESYREDPDSVDERTREFFESWSP
                     PRLEAPGANGHVAPEVDVEKAVGASKHVRHIRDFGHRAARLDPLGSEPPGDPTLDPTF
                     YRITDEDLETLPTLIIGGPIAERTSNAREAVDELRGLYCDTTGYDFGHVHDPEERFWL
                     RESVESGRFNKRMEGEDAKRLLQRLTRVDAFEKFLHKTFLGQKRFSVEGNDMVVPMLN
                     RLIRSAADVGTPVTTLGMAHRGRLNVLAHVMDKPYSKIFGEFQQPEKGEQSSAAGRPG
                     DAWVGDVKYHLGVRGFQLADEEADEEVFIHLAPNPSHLEHVNPVVEGMARASQEETDE
                     AGPPLQDVDSSLPILLHGDAAFPGEGVSAETLNLSRLPGYRTGGTIHIITNNQLGFTT
                     ERQDARSTTYASDLAKGFEIPVVHVNADDPEACMAAARMAHAYREKFNKDFMIDLVGY
                     RRFGHNEGDEPAYTQPLMYETIRNHPTVREIWAEELERRGVLDEGEAEAMFEEIMSAM
                     AEVQKKATDELKEEDFAADDLYTPLVGIPETAVAAERLSELNEALLNSPEEFAPNSKL
                     ERLF"
BASE COUNT          291 a          588 c          568 g          370 t
ORIGIN      
        1 ggaagagccg ctcgagcttg gagttcggcg cgaattcctc ggggctatta agtagggctt
       61 cgttcagttc agagagcctc tcggccgcaa cagcggtctc ggggatcccg accagcggcg
      121 tgtacagatc gtccgcggcg aagtcttctt ctttgagttc gtcggtggcc tttttttgga
      181 cctcggccat ggctgacatg atctcctcga acattgcctc tgcctcgccc tcatcaagga
      241 cgccgcggcg ttcgagttct tcggcccaga tctcacgcac cgtggggtgg ttgcggatgg
      301 tctcgtacat cagcggctgc gtataggcag gctcgtcgcc ctcgttgtgc ccaaaacggc
      361 ggtagccgac gaggtcgatc atgaagtcct tgttgaactt ctcgcggtaa gcgtgggcca
      421 tcctcgccgc cgccatgcac gcctcgggat cgtcggcgtt gacgtggacc acggggatct
      481 cgaacccctt ggccaaatcg ctcgcgtagg tcgtcgagcg ggcgtcttga cgctccgtgg
      541 taaacccgag ctggttgttg gtaatgatgt ggatggtgcc gcctgtcctg tagccaggca
      601 gacggctcag gttcaaggtc tcggccgaga ccccttcgcc agggaacgcc gcgtccccgt
      661 gcaagaggat gggcaacgat gagtcgacgt cctgaagcgg aggtcctgct tcgtccgttt
      721 cctcctggga ggcgcgggcc atcccctcga ccacggggtt cacgtgctcg aggtggctcg
      781 ggttcggcgc gaggtggata aagacctcct cgtcggcctc ctcgtcggcg agctggaagc
      841 ccctgacccc gaggtggtac ttcacgtccc cgacccacgc gtcgccgggc ctgccagcgg
      901 cggaggactg ctcgcccttc tccggctgct ggaactcgcc gaagatcttc gagtaaggct
      961 tgtccatcac gtgcgcgagg acgttgaggc gtccgcggtg cgccatgcca agggtggtca
     1021 ccggcgtgcc gacgtccgcg gcggagcgga tgagcctgtt cagcatcgga acgaccatgt
     1081 cgttcccctc caccgagaac cgcttctggc cgaggaaagt cttgtggagg aacttctcga
     1141 aggcgtctac ccgcgtgagc ctctggagga gccgcttcgc gtcttcaccc tccatcctct
     1201 tgttgaagcg ccctgactcg acggactcgc gcagccagaa cctctcctca gggtcgtgga
     1261 cgtgcccgaa gtcgtatccc gtggtgtcac agtagagccc gcgcaactca tcaacagcct
     1321 ccctggcgtt ggaggtacgc tcggcgatgg gaccgccgat gataagcgta ggcagggtct
     1381 cgaggtcctc gtccgttatc ctgtaaaacg tcgggtcgag cgtcgggtca cctggcggct
     1441 cgctcccgag agggtccagc ctcgctgctc tgtgaccgaa gtcccttatg tgcctcacgt
     1501 gcttcgaagc cccgaccgcc ttctcgacgt cgacctcggg cgcgacgtgc ccgttggcgc
     1561 ctggcgcctc caaacgcggc gggctccagg actcgaagaa ctcccgcgtc cgctcgtcga
     1621 cggagtccgg gtcctccctg tagctctcgt agagttccaa cacgtagccg aggttcggcc
     1681 cgaagaactg atactctgtc tgtcgctcca accggatcac accctcacgt gatgatacag
     1741 cccgagcgtc gcacggaggg gcgcgacatc cgtccgcctt cgaacaacgc tctttgcaag
     1801 gcaattgaac atcttgt
//
LOCUS       DATAIR010000532         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_35662, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000532 DATAIR010000000
VERSION     DATAIR010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35662"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VHM69_12610"
     CDS             complement(<1..439)
                     /locus_tag="VHM69_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741281.1"
                     /translation="MDNQNIVMGGKTVLITGGTSGIGKATALALAAMGANVVISGRNA
                     ERAERAMEEIKGQSHNQSVDLILADLSVQSEVRRLAEAFLARHDSLDVLANNAGLVLN
                     RRTETPDGIETTLAINHLAPFLLTNLLLDRLRESAPARIVTTSS"
     gene            complement(537..710)
                     /locus_tag="VHM69_12615"
     CDS             complement(537..710)
                     /locus_tag="VHM69_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741282.1"
                     /translation="MRSMREAAEYSRNLTSAGDVQITPERSPENQEWIWMVAEDPAFY
                     LANSAISRENAGG"
     gene            complement(707..1564)
                     /locus_tag="VHM69_12620"
     CDS             complement(707..1564)
                     /locus_tag="VHM69_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2741283.1"
                     /translation="MKSITVGSVEITALTDIEGAFFRLDQIFPGVMQDQWEPYLHRYP
                     WAFADGETLRGRVGSYLLRSPESTVLVDAGVGPQAMGAGGKLLVELENAGVHPEDVDT
                     VFLTHLHGDHLGWSVKPDGEPVFPEARYVTQASEWKISAPYLGRAFAALDDLGVLELL
                     DGEEPVGEELTAIPTPGHSPGHSSLLVSSGGEQALVSGDTIVHPAQVTEPTWNVQFDM
                     DKEQAARTREMLLAWLEADGIIVAAGHIPGSGFGRIVREEPERRYWRTLETAQETEDL
                     IDLFRGGRS"
     gene            complement(1568..>2390)
                     /locus_tag="VHM69_12625"
     CDS             complement(1568..>2390)
                     /locus_tag="VHM69_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2741284.1"
                     /translation="VLLGWREIRSLDLSRWGALIFVGWGGSAVATILFTTGLFKAFEA
                     GAGSVNTVLLLQQTQPLFAIAAAAVVLGERLTVLYAPIFAVAAIGAYLLAFGDLIGEP
                     GGLFAPFTQVGRARLEFALAALGAAALWGTSTAMGRYLGGRLSFQTLTGARFLVALPL
                     LLVWAAIAIPDAAGSLAGGLAEPQVARKLVLLALFPGLLGLLLYYRGLRSTPASYATL
                     AELAYPATGVTLNWLLLGQPLTVAQGVGVALVVGAIAAMNRVKSGVRVRRSSQGG"
BASE COUNT          452 a          805 c          732 g          401 t
ORIGIN      
        1 ccgaagaggt cgtgacgatg cgggcaggcg cgctctccct gaggcggtcg agcaggaggt
       61 tcgtgagcag gaacggcgcg aggtggttta tggccagcgt cgtctcgata ccgtcgggcg
      121 tctctgtccg cctgttcagc acaagccccg cattgttggc gagaacgtcg aggctgtcgt
      181 ggcgcgcgag gaacgcttcg gccaaccgac ggacctcgct ctgcacggac agatcagcga
      241 ggatcaaatc tacggattga ttatggctct ggcctttgat ctcctccatt gcccgctctg
      301 cgcgctccgc gttacgtcct gagatgacga cgttcgcacc catcgccgcc aacgccaacg
      361 ccgtcgcctt cccgatgccg gaagtgccgc ccgtgatcag gaccgtcttg cctcccataa
      421 caatgttctg attatccaag atctctccca gaaggctcgc atctgccctg agtctatacc
      481 gcctcggcgg tccagtgtcc cccgtaccag caacactccg agagcggagc gttactctat
      541 ccgccagcgt tttcgcgcga tatggcggag ttcgcgaggt agaaagcagg gtcttcggcg
      601 accatccaga tccactcctg gttctccggg ctccgctccg gtgtgatctg cacgtcgcca
      661 gcgcttgtga ggtttctcga atactccgcg gcctctctca tgcttctcaa gacctacctc
      721 ctctgaacag gtcgatgagg tcctccgtct cctgcgccgt ctccagcgtc cgccagtagc
      781 ggcgttccgg ctcctcgcgc acgatacgcc cgaagcctga gcctgggatg tgccctgctg
      841 cgacgatgat accgtcagcc tccagccacg cgagcaacat ctcgcgggtg cgggcagcct
      901 gctccttgtc catgtcgaat tggacgttcc aggtcggctc cgttacctgc gccggatgca
      961 cgatggtgtc gccagagacg agcgcctgct ccccgcctga ggagacgagc aaactactgt
     1021 gtcccggcga gtgacccggg gtcggtatgg ccgtgagctc ctcgccaaca ggctcctcgc
     1081 catcgagcag ctccaggaca cccaggtcgt cgagtgccgc gaaggcgcgc cccagatacg
     1141 gtgccgagat cttccactcc gatgcctgcg taacgtagcg ggcctcgggg aagacgggct
     1201 cgccgtctgg cttcacgctc cagccgagat ggtcgccgtg cagatgggtc aggaagacgg
     1261 tgtcgacgtc ctcagggtgg acacccgcgt tctcgagctc gacgagcagc ttgccgccag
     1321 cgcccattgc ctgcggacca acgccggcgt ccaccaggac cgtgctctca ggggaacgca
     1381 gtaggtaaga cccgacgcgc ccacgtaagg tttcgccatc agcgaaagcc cacgggtatc
     1441 ggtgcaggta gggctcccac tgatcctgca tgacgccggg gaagatctgg tcgagccgga
     1501 agaaagcccc ctcgatgtcg gtcagagctg taatttcgac gctgcccacg gttatacttt
     1561 tcaagatcta acctccttga gacgagcgcc gcacgcgcac gcctgacttg acgcggttca
     1621 tcgccgctat agccccaacg acgagcgcga cgccgacgcc ctgtgcgacg gtgagcggtt
     1681 gtccgagcaa caaccagttg agggtaaccc ccgtcgcggg atatgcaagc tcggcgagcg
     1741 tggcgtagct tgcgggggtg ctcctcaaac ccctgtagta caagagtaga ccgagaagcc
     1801 ctgggaagag agcgagcaac accagcttgc gcgcgacctg cggttcggcg agccctcctg
     1861 caaggctgcc agcagcgtcc gggatcgcga tggcggccca cacgagaagt agcggcagag
     1921 cgacgaggaa gcgggcgccc gtgagcgtct ggaaagagag ccgcccgccg aggtaacgtc
     1981 ccatcgcggt cgatgtgccc cacagcgcag ccgccccgag cgccgcgagg gcgaactcga
     2041 gccgcgcccg cccgacttgc gtgaacgggg cgaacaggcc gccaggctcc ccgatcaaat
     2101 ccccaaaggc cagcagataa gccccgatgg cggcgacggc gaagatcggg gcgtacaaca
     2161 cggtcagccg ctctcccaag accacggccg ccgccgcgat ggcgaatagt ggctgcgtct
     2221 gttgcagtag caacacggtg ttcacagaac ccgcaccagc ctcgaacgct ttgaacagac
     2281 ccgtggtaaa caggatggtg gcgaccgcgg acccacccca cccgacgaag atgagagcgc
     2341 cccaccgcga caggtccaga ctccgaatct cacgccaccc aagcagtaca
//
LOCUS       DATAIR010000533         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_200980, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000533 DATAIR010000000
VERSION     DATAIR010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200980"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1647)
                     /locus_tag="VHM69_12630"
     CDS             complement(130..1647)
                     /locus_tag="VHM69_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2741285.1"
                     /translation="MGLSKRRKALITLATLIGTFLAALDATVVGTAMPTVIGELGGLE
                     LYPWAFASYLLAATVTGPVFGKLSDTYGRKPVYLAGIFLFLLGSVLAGTSQSMAALIL
                     FRTLQGLGAGAVQPVAITIVGDIFELEARARIQGLFGAVWGISAVVGPAAGGFITDLI
                     SWRWVFYVNVPFGIVAAVLLSMTLTETFERRTRGVDYLGTALLTGGLVAVLLALLGGG
                     TVLSASTLALFFGGVAVLALFVLVERRAADPIVPLDLFKERIIAVSALGNLAMGGVLL
                     GVSVYVPLFVQGALGGTAITAGAAVASLSIGWPVGSFVGGRMLLLTGYRTTLLLGSAL
                     IALGSALCVPMDGGTSLAYVVLAVVVIGLGLGFSSTSYLVSVQNAVPWRRRGVATSSV
                     VFFRTVGGLLGVAVMGALLNASLGERYRAAVDKAAGGDAVLERLLSDPNALLQPAVRS
                     RIPEAVYSELAGALAASLSPAFWVLLFFGLSALAVATLFPHGTAKDLVSRDEDHG"
     gene            complement(1709..2845)
                     /locus_tag="VHM69_12635"
     CDS             complement(1709..2845)
                     /locus_tag="VHM69_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2741286.1"
                     /translation="MTKAVVIGGGIGGLTAAISLRRAGVEAAVFERAPELREVGAGIA
                     LWANAAKALRKLGLYEAVRDRSAEIGGETRTWRGRKLFTITADEMRSRFGEANLVMHR
                     ADLQATLLAALPEDAVRLGAKLESFSQDGEAVVAHFSGGREETADFLVGADGLRSTVR
                     ARLLGDGPPRYAGFMAWRGIAEGVEGLVPEGIGLNLWGRGMEFGLISIGRGRAYWYLS
                     KNAPEGGVEGPNKEEVLRNLRGWYGAARAAVQATEDSGILRNDIYDREPARRWGEVNA
                     TLLGDAAHPMTPNLGQGACQAIEDAVVLADAVRQTDSAAAALRSYEARRIRRTGAVVR
                     ASRNMARVMQIEAPLLCSVRDAGFRLMPAGVMMGRLDPIVRYEV"
     gene            complement(2942..3331)
                     /locus_tag="VHM69_12640"
     CDS             complement(2942..3331)
                     /locus_tag="VHM69_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741287.1"
                     /translation="MDRRATIMVADDDAEALKRSAGALENAGFRVVSATDGRTALQEA
                     LTRRVDLIIMDVSIPQLSGVETCHCLKAMPKTQKIPVILTAAKKDPAAKALAQRTHGS
                     VRVLRKPFSDEDLVSVVQQLARPKSLI"
     gene            3396..4682
                     /locus_tag="VHM69_12645"
     CDS             3396..4682
                     /locus_tag="VHM69_12645"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2741288.1"
                     /translation="MDALHSTSSQEHERRVREVSVTEQKYFRKGFKMRENVQHLLDRD
                     YRSEVVDRVRENGNVLAAENLTIRLADEFGFCYGVDRAVDYAFQTREKFPNRRIFITG
                     DMIHNASMNDRLREMGIEFLSTGFDGPPDQAFDMLSPEDVVLLPAFGAPIDWVEKLRE
                     KECVVVDTTCGSVLSVWKRVTRYAKRGFTSIIHGKYYHEETKATASQTYRDDGNGKYL
                     IVRNLKETGHVTDFILGRMGAEELREKLGHGMCPGFEPDEDLQRVGVANQTTMLMSET
                     MAVGEELRKAMAERYGAQNVEEHFELFDTICSATQDRQDALFELLENPLDVVIVIGGY
                     NSSNTNNLAIIADERVPHSYHIASGDCIDGETIHHKPAGTPLDSREEVDEHGWLPDGP
                     VRIGLTAGASTPNSQIGLAIGRILEARGLPPETALS"
     gene            complement(5107..5316)
                     /locus_tag="VHM69_12650"
     CDS             complement(5107..5316)
                     /locus_tag="VHM69_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741289.1"
                     /translation="MKKVLRRLFLLGALAGAAFAARSYLKGSTPVKESVHIEFDDGSK
                     RTLASNTVEGQEFTDIATKLVEIGL"
     gene            complement(5313..5714)
                     /locus_tag="VHM69_12655"
     CDS             complement(5313..5714)
                     /locus_tag="VHM69_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX2741290.1"
                     /translation="MAERSGAGATEELDRVLADLRALSEDIESCAVLSGTRDLLSSSH
                     GPGVDRERVGAMLAALVNLSERVARENGKDHTSQLRVDTDDGHLLLVRLDGGGALAAT
                     TGPEARVGLVLYDMRNSRGEVEKVIAEGDKS"
     gene            5822..5893
                     /locus_tag="VHM69_12660"
     tRNA            5822..5893
                     /locus_tag="VHM69_12660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5854..5856,aa:Val,seq:cac)
     gene            complement(5925..6542)
                     /locus_tag="VHM69_12665"
     CDS             complement(5925..6542)
                     /locus_tag="VHM69_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2741291.1"
                     /translation="MVTEIITVSVKPGMEEAYRDWVDRIRQMEAKFPGYQGLQLQPPI
                     PGLQDDWVSLLRFDTSEHLNAWLESDARRTALRDVESFIDQREQQVATAFSGWFTFGD
                     APDQVPPNWKQSMIVLLMLYPVVMLELLYLNPLLRPLGMAEGTFIGNLLSVAALGYLL
                     VPRANRVFDWWLRPTSKNSPGVEAAGIALIIGLYALSIVVFAWIS"
BASE COUNT         1301 a         2158 c         1991 g         1138 t
ORIGIN      
        1 aagagtacgc cgaccctggc gctttctcca ccgacttcgg tctgctggaa gacgtccctt
       61 ccctcggggt cgaccagttt gaagagcggc ggacgaggga ggtcgtccag ggtcagctct
      121 cgtcctgtct cacccatgat cctcgtctcg actcaccagg tctttggctg ttccgtgcgg
      181 gaagagcgtc gccactgcga gggctgagag accgaagaag agcagcaccc agaacgccgg
      241 ggagagcgag gctgcgaggg ctccggccag ctccgagtac acagcttcgg ggatgcggct
      301 ccgcacggcg ggctgcagaa gggcgttcgg gtcggagagc agacgctcga ggaccgcgtc
      361 gccccctgcg gccttatcca cggcggccct gtaacgctcc ccgagagaag cgttcagcaa
      421 ggcgcccatc acggcgaccc cgagcaagcc gcctaccgtg cggaagaaga ccacagaaga
      481 agtcgcgacg ccgcggcgcc gccagggcac cgcgttctgt acgctgacca gatagctcgt
      541 gctcgagaag ccaagcccga gcccgattac gacgacggcg aggacgacat aggcgaggga
      601 ggtcccgcca tccatcggca cgcagagggc cgaacccagg gcgatcaggg ccgagccaag
      661 gaggagcgtc gtgcggtatc cagtcaggag gagcatcctt ccgccaacga aagatcccac
      721 gggccacccg atggagagcg aggccaccgc cgcgcctgcg gtgatggccg tccctcccag
      781 tgcgccctgt acgaagagcg gcacgtagac ggagacgccg agcaacacgc cgcccatcgc
      841 caggtttccg agggccgaga cggcgatgat ccgctccttg aacaggtcga gcgggactat
      901 gggatcggca gcccgccgtt cgacgagaac gaagagcgcg agcacggcca cgccgccgaa
      961 gaagagcgcc agggtggagg ccgagagcac ggtcccaccg ccgagaagcg cgagcaggac
     1021 cgcgacgagg ccgcctgtga ggagggccgt tccgaggtag tccacgccgc gcgtccggcg
     1081 ttcgaacgtc tcggtcaggg tcatggagag gaggacggcg gcgacgatac cgaacggtac
     1141 gttaacgtag aagacccagc gccaggagat caggtccgta ataaagccgc ccgcggcggg
     1201 gccgaccacg gcggagatcc cccacaccgc cccgaagaga ccctgtatgc gagcccgcgc
     1261 ctcgagctcg aagatgtccc cgactatcgt aatggcgaca ggctgcacgg cccctgcgcc
     1321 gagaccctgg agcgtcctga agaggatcag ggcggccatg ctctgggatg tgcccgcgag
     1381 cacgctaccc agcaggaaga ggaagatccc agccagatag acgggcttcc tgccgtaggt
     1441 gtcggagagt ttgccgaaga cgggtcctgt cacggttgcg gccaggaggt aagaggcgaa
     1501 ggcccacggg tagagttcga ggccgccgag ctccccgatc accgtcggca tcgccgtacc
     1561 cacgaccgtc gcgtcgaggg ccgcaagaaa cgtcccgata agcgtggcga gcgttatgag
     1621 tgccttgcgg cgcttgctca gtcccaagga ccagcacctc ttccgcgatc tacgtctttt
     1681 acggggtgcg atcttaccac cgcagacttc atacctcgta cctgacgatg ggatccaacc
     1741 tccccatcat gaccccggca ggcatgagcc tgaaccccgc gtcgcgcaca ctgcaaagca
     1801 gaggagcttc gatctgcatg actctcgcca tattccgcga cgcccgtacg acggcgccgg
     1861 tacgccggat gcgcctcgcc tcgtaggaac gcagggctgc cgccgccgag tccgtttggc
     1921 gcacggcgtc agccaggaca acggcgtcct ctatcgcctg gcaggcgccc tgtccgagat
     1981 tcggcgtcat cggatgggcc gcgtccccga ggagggtcgc gttgacctcg ccccagcgtc
     2041 gggccggctc gcggtcgtag atgtcgttgc gcaggatccc cgagtcctcc gtcgcctgta
     2101 ccgccgctct cgcggccccg taccagcctc gaaggttcct gagtacttcc tccttgttcg
     2161 gaccttcaac cccgccctcg ggcgcgttct tgctcaggta ccagtaagcg cgtccgcggc
     2221 cgatgctgat caatccgaac tccatgcctc ttccccagag gttgaggccg attccttcgg
     2281 gaacgagtcc ttcgacgccc tcggcgatgc ctcgccaggc catgaacccg gcgtagcgcg
     2341 gcggaccatc acccaggagt cgcgcccgta ccgtcgagcg caggccgtcg gctcccacga
     2401 gaaaatccgc cgtctcctcc ctgcctccgc tgaaatgggc taccacggcc tctccatcct
     2461 gggagaaaga ttccagtttg gcccccagcc tcacggcgtc ttcaggcagg gctgccagaa
     2521 gcgtggcttg caggtccgcc ctgtgcatga ccaggttggc ttctccgaac ctgctcctca
     2581 tctcgtcagc agttatggtg aagagcttcc tacctcgcca ggtgcgcgtc tcgcctccga
     2641 tctctgcgga tcgatcccga accgcttcgt agaggccaag ctttcgcagc gccttcgcgg
     2701 cgttggccca cagtgcgatc cccgccccga cctcccgcag ctcgggcgct cgctcgaaca
     2761 ccgcggcctc cacgcctgcc ctccgcagcg agatcgccgc cgtgagcccg ccgatcccac
     2821 cccctatcac cacggctttc gtcacgagat cctccgatct ttaacgaagc acagacaggt
     2881 tagaccgtcg ggcgaaaccc ttcggtaaac gtgttaccag ccgggagggg agttaaggaa
     2941 gttagatcag ggacttgggc cgtgcgagtt gttggacgac ggagacgagg tcttcgtcgg
     3001 aaaagggctt cctcaggacc cgtacagagc cgtgcgtacg ttgggcgagt gctttcgcgg
     3061 ctgggtcttt cttggcagcg gtgaggatga cagggatttt ctgggtcttc ggcattgcct
     3121 tgagacaatg acacgtctcg acgccgctaa gttgggggat cgaaacgtcc atgattatga
     3181 ggtcgaccct gcgggtgagg gcctcctgga gagccgtgcg gccgtccgtg gccgaaacca
     3241 cgcggaatcc agcgttctca agggctcctg cggagcgttt gagcgcttcg gcgtcgtcgt
     3301 ctgcgaccat gatggtggct ctgcggtcca cggcgctaga ttttacaccc atttcacatt
     3361 taaagcgaca gtgacctgcg ccacgacacg gctttatgga tgcgttacac tcaacttcga
     3421 gccaggaaca cgaacgtcgg gtacgagagg tgagcgtgac ggagcaaaaa tatttccgca
     3481 aaggcttcaa gatgcgggag aacgtccagc atctcctgga ccgggactac cgctctgaag
     3541 tcgttgacag ggtgcgtgag aacggcaacg tcctcgctgc cgaaaacctc acgattcgcc
     3601 tcgccgacga gttcgggttc tgctacggag tcgacagggc cgttgactac gccttccaga
     3661 cccgcgagaa gttccccaac aggcgcatct tcatcaccgg agacatgatc cacaacgcct
     3721 ccatgaacga ccgtctgcgc gagatgggaa tagagttcct ctcgaccggc ttcgacggtc
     3781 cccccgacca agctttcgac atgctctccc ccgaggacgt cgtgctcctc ccggccttcg
     3841 gcgcccccat agactgggtc gagaagctca gggagaagga atgcgtcgtc gtggacacga
     3901 cgtgcgggtc ggtcctctcc gtgtggaagc gcgtaacgcg ctacgccaag cgcggcttca
     3961 ccagcatcat ccacggcaag tactatcacg aggagacgaa ggccacagcc tcccagacgt
     4021 acagggacga cggcaacggc aagtacctca tcgtccgcaa cctcaaagag acgggccacg
     4081 tgactgactt catccttggc aggatgggtg ccgaggaact cagggagaag ctcggacacg
     4141 gcatgtgccc aggcttcgag ccggatgaag acctgcagcg cgtcggcgtc gccaaccaga
     4201 caacgatgct gatgagtgag acgatggccg tcggggagga gctcaggaag gcgatggcgg
     4261 agcgctacgg cgcccagaac gtcgaggagc acttcgagct cttcgacacc atctgctcgg
     4321 cgacccagga caggcaggac gccctcttcg agctgctgga gaacccactg gacgtcgtga
     4381 tcgtcatcgg cggctacaac tcatccaata cgaacaacct ggcgatcatc gccgacgaga
     4441 gggtcccgca cagctaccac atagctagcg gagactgcat agacggtgaa accatccacc
     4501 acaaacccgc aggcacgccc ctggacagcc gggaagaggt agatgagcac ggctggctcc
     4561 ccgatggtcc cgtgcggatc gggctcacag ccggcgcctc gacgccgaat tcccagatag
     4621 gactagccat cgggcgcatc ctcgaggcgc gcgggctccc gccggagacc gcactcagtt
     4681 aaaacgaata tcagaagata tcttctgata ttcgcgctat caggtatcag cagtcagctt
     4741 cttgttctct ctccgcaaag tgctctatgt ggccttcggc gccgcttccg agcagtctcc
     4801 cagcttatcc gacgaagtag gtagcttcac atccatggag gtcccaatca agctctatgc
     4861 ggctcgtcga gccatcaaga aacgcgaggg gtggcagtgg atccaaaacc ggacctgaaa
     4921 acgttgagca cgtgatccgg gacccacacg gagtccaggc ccggctccta cgcagccctg
     4981 accatcccga cgtacgcctc gagaccttca cccccaggtc gtccggatca aacatgtcga
     5041 gcgcccggag tgccctgata cctgaagcct cggatttagc gagaggatca cctctcgcga
     5101 tccgccttag agcccgatct ctacgagctt ggtggctatg tctgtaaact cctgtccttc
     5161 gacggtgttc gaggcgagcg tgcgcttaga gccgtcgtcg aactctatgt ggacggactc
     5221 tttcaccggt gtgcttccct tcaggtagct acgcgcggcg aaggcggcgc cggccagcgc
     5281 cccgagcaga aagaggcgtc tcagaacctt cttcatgact tgtccccctc cgcgatgact
     5341 ttctcgacct cgccgcgaga gtttcgcatg tcgtagagca cgagtccaac cctggcttct
     5401 ggccccgtcg tggccgccag cgcgcctccg ccatcgagcc gtaccaacag taggtgtcca
     5461 tcgtccgtat ctactcttag ctgggaggtg tggtccttcc cgttctcccg cgccaccctc
     5521 tccgagaggt tcaccagcgc ggcgagcatc gcgccgacgc gctccctatc cacccctggc
     5581 ccatgtgagg acgagaggag atcccgcgtt ccagacagaa ccgcgcacga ctcgatatct
     5641 tccgagagtg ccctgaggtc agccagcacg cggtccaatt cttccgtcgc gccggctccg
     5701 cttcgttccg ccataagtat ctccgtcccc tgtttaccgt cgaagcgggc attatatccg
     5761 atggagatcc tcccaacgaa cttaacacaa acccccgata atggtatatt caccgctcca
     5821 ggggcgatta gctcagcggg agagcgctgc cttcacacgg cagaggtcgc tggttcgagc
     5881 ccagcatcgc ccacttcgag tcggttgtgc tcgacttggt gacctcaact gatccatgcg
     5941 aacacgacaa tagacagggc gtacaggccg atgatcagcg caatcccggc ggcctcaacc
     6001 cccggagaat tcttggacgt tggacgtagc caccagtcga agacgcggtt ggcccggggc
     6061 accaacaagt atcccagtgc tgcgacgctg agcaggttgc cgataaaggt gccttccgcc
     6121 atgcccagcg ggcgcaggag tgggttgaga tacagcagtt cgagcatcac gaccgggtac
     6181 agcatcagca gcacgatcat ggactgcttc cagttgggtg gcacttgatc gggagcatca
     6241 ccaaaggtaa accatcccga gaaggccgtg gcgacctgct gttcgcgctg atcgatgaat
     6301 gactcgacat cccgcaaggc ggtacggcga gcatcggact ccagccaggc gttcaagtgc
     6361 tctgacgtat cgaagcgtag cagcgagacc cagtcatcct gcagcccggg gatcgggggc
     6421 tgcaactgta gtccctggta tcccggaaac tttgcctcca tctgcctgat acggtccacc
     6481 cagtcacggt acgcctcctc cataccaggc ttgacgctga ctgtgatgat ctcggtcacc
     6541 atatcctgtg gggagcgagc gctccgtaga tgctccttcg atcacgtt
//
LOCUS       DATAIR010000534         4802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_62461, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000534 DATAIR010000000
VERSION     DATAIR010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62461"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHM69_12670"
     CDS             <1..341
                     /locus_tag="VHM69_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2741292.1"
                     /translation="LKRSETGARGKIIEAGDLQLDTKNFTAHVAGEPLALRLKEFELL
                     AALASTPGELRSREELAKEVWWHAGVGSSRTIDVHIRRIRAALEQNSAFEYIHTARGL
                     GYRFEPKHRE"
     gene            639..1088
                     /locus_tag="VHM69_12675"
     CDS             639..1088
                     /locus_tag="VHM69_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2741293.1"
                     /translation="MRGVLMTVLARMRRVARKKDITLQWKRFGKAAQYTVYGDETQLT
                     SMFTNLVDNAVKYTPPGGRVEVIGGSEDSEIVIRISDTGIGISEGQIPRIFERFYRVD
                     KARSKATGGTGLGLSIVRHVAQNHGGRVTVESAPGEGSTFTVYLPRA"
     gene            1278..>4802
                     /locus_tag="VHM69_12680"
     CDS             1278..>4802
                     /locus_tag="VHM69_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741294.1"
                     /translation="MESERSGRFRAGNRALWVALCLAVVLWFLDAAMEALVFHEGTFA
                     DRLFTVEPSEVWHRLAITAGVIAFGILIQRGINRSRPTRAALKEGEERFRSLSDATFE
                     GIAISENGRILETNRAFAKMYGYETSEIVGMSAEALIAPESREVALGHIFSDSEEPYE
                     AVALKKDGTKFTVEVRGKKSSYRGRPVRITALRDITERRESEKRLREAEERYRTVVEQ
                     IPAATYVQEIEHDFASTFVSPQIEEIVGYTPHEYLSTPNLWIDILHPEDRERVLAEDA
                     RTDETGEPFRVEYRMICKDGRVVWVRDEAVIVRGDEGAPLYWQGFMQDITERKRAEDA
                     LLENEERLRLALEAGSLGTWQIDLERGEFTAFPRARAMHGLTEETLDNQKLIEAVHPE
                     DREEIRQRMERAITGGGSYEAEYRIAWPDGTYRWLDSRGRVYGRDERGRGGRLIGVVR
                     DVTERKRTERRIAAQYATTHILEESTTLKEASPRLLRELCENLEWEMGALWEVDQHVL
                     RCARTWVAPTMRDSEFEAATRGRTFSRGEGLPGRVWAGGEPAWVQDVLEDDNFPRAPL
                     AAKAGLHGAFAIPIVLRGEVLGALEFLSYEVRQPDEPTLAMMGSVGIQLGQFVERRRA
                     QEELREAEERFRSAFDDAAIGMALNTVDGRFVQVNAALCKMLGRSEDELLASTFQDIT
                     YPEDVDASVMRVRQLLEGEIQSYQLEKPYPHADGHTVWISLNVSLVKDSKGEALYLVS
                     QMQDVTDRKRAEESLRASEAELRAVFSAMNDVILVLDAEGRYLEIAPTNPSLLYKPSE
                     DLIGKTLHEIFPAEQADVFLDFVRRALLEQRPVNLEYSLMIDGRETWFAGTVSAMEED
                     RVVYVARDITESKRAQDELRKSEERTRTIVETTPDAIITMSKDGFIQSFNPGAERIFG
                     YTTGEAVGQPLTMLMPERFKAQHEAGFRRHLDTGEAHIVDKGAVEFVGRRKDGEEFPI
                     ELSLGGMHEGDDVLFTGIVRDITERKKAEEDLRQSEERYRLVAQATDETIWDSDILAD
                     EQAWDGAVETMFGYSSEQRTETTWWEERIHPEDRERVLSSVDSALEGGVDMWSEEYRF
                     MHADGSYSTVVDRAYVVRDAEGKAVRMIGSMADVTERRRAERELRENEKRFRQLFDQS
                     VDALFVHDASGKIVDCNAEACRSLGYSRE"
BASE COUNT         1027 a         1445 c         1594 g          736 t
ORIGIN      
        1 acctgaaacg cagcgagacg ggcgcgaggg gcaagatcat cgaggcggga gacctgcaac
       61 ttgacacgaa gaattttact gcccacgtcg cgggagaacc gctcgcgctg cggctgaagg
      121 agttcgagtt gctcgccgcc ctggcctcga ccccgggcga gctgaggagc cgcgaggagc
      181 tggcgaagga ggtctggtgg cacgccggag tcggttcctc ccgcacgata gacgtgcaca
      241 tacgcaggat cagggccgcc ctggagcaaa actcggcctt cgagtacatc cacaccgccc
      301 gcggcctcgg ctaccgcttc gagccgaaac acagggagta gacgctgcag agacgaaacg
      361 gcacgcactc cgtcgacccc gatgaactcc agagggtcaa caagatccgg cgcgacttcg
      421 tcgcgaacgt ctcccacgag ctgaagacgc cggcgacgag cctgaagctg ctggccgaaa
      481 gcctggagga gaccctcgac gaggacccgg tccaggcgcg tctcttcgcc gcgcaactca
      541 agaaggagac agaacggctc gcccacctca taacggacct cctcgacctg gcgcgcctcg
      601 agagccagga gtggatcgac taccccaccc tcgttgacgt gcgcggcgtc ctgatgaccg
      661 tgctcgcccg catgaggcgc gtcgcccgca agaaggacat cacactccag tggaagcgct
      721 tcggcaaggc cgcccagtac accgtctacg gcgacgagac acaactgacc tccatgttca
      781 ctaatctggt ggataatgct gtcaagtaca cgccgccggg agggcgggtc gaggtgatcg
      841 gtggttccga ggactccgag atagtcatac gcatctccga cacgggcata ggcatctcgg
      901 agggtcagat cccacgcatc ttcgagcgtt tctaccgcgt ggacaaggcc cggtcgaagg
      961 cgaccggcgg gactggactg ggcctatcca tagtgaggca cgtggcccag aaccacggcg
     1021 gccgggtcac cgtcgagagc gccccgggcg aagggtcaac cttcaccgtc tatctcccgc
     1081 gcgcctgaga gcccacaggg caagacaacg aatgtactgt atccatcaaa gtactaacac
     1141 cgggtactag catttgggta tacttccggt cgagccggct cggggaacgt cttcggagga
     1201 acgccgaaga ctaacacgaa tagcaggcga cgttgcgcga gcggtgcacg agcacatcgg
     1261 atttagagag gcacaggatg gagagcgaga gatccgggcg tttccgcgca ggcaaccgtg
     1321 cgctgtgggt tgctctctgc ctcgccgtag tcttgtggtt cttggacgcc gccatggagg
     1381 ccctcgtgtt tcacgaaggg accttcgccg acaggttgtt caccgtcgag ccgagcgaag
     1441 tgtggcaccg tctcgcgata accgccgggg tcatcgcttt cggcatcctt attcagcgtg
     1501 gcatcaaccg gagcaggccg acccgggcag cgttgaagga gggtgaggaa cgcttcagga
     1561 gcctctccga cgccacgttc gagggcatag ccatcagcga gaacgggagg atcctggaga
     1621 ccaaccgggc ctttgccaag atgtatggtt acgagacctc cgagatcgtc ggcatgtctg
     1681 cggaggccct cattgcgcca gaatcccgcg aagtagcgct ggggcacatc ttctctgact
     1741 ccgaggagcc atacgaggcc gtagctctca agaaagacgg cacgaagttc accgtggaag
     1801 tcagggggaa gaagtcgtcg taccggggac gtcccgtccg gataaccgcc ctgcgggaca
     1861 ttacggagcg cagggagtcg gagaagagac tcagggaggc cgaggaaaga tacaggactg
     1921 tcgtcgagca gatccccgcc gcaacatacg tgcaggagat cgagcacgac ttcgcgagca
     1981 cgttcgtgag ccctcagata gaggagatag tgggatacac cccgcatgag tacctgtcca
     2041 caccgaatct gtggatagac atccttcacc ccgaggacag ggagcgtgtc cttgccgagg
     2101 acgcgcgcac ggacgagaca ggcgaaccct tcagggtgga gtacaggatg atctgcaagg
     2161 acgggcgcgt cgtttgggtg cgcgacgagg cggtgatcgt acgcggcgat gagggcgccc
     2221 ctctctactg gcagggcttc atgcaggaca tcaccgagcg caagagggcc gaagacgcct
     2281 tgctggagaa cgaagaacgg ctaaggctgg cgctggaagc aggctcgctc ggcacgtggc
     2341 aaatcgacct ggagcgtggt gagttcacgg ccttcccacg ggccagggcc atgcacggcc
     2401 tgacggagga gacgctcgac aaccagaagc tgatcgaggc ggtacaccct gaggaccgcg
     2461 aagagataag gcagagaatg gagcgggcga tcacgggggg agggtcctac gaggcagagt
     2521 accgcatcgc atggcctgac ggcacctacc gctggctgga ctcccgcggg cgggtctacg
     2581 gtcgcgacga aaggggccgc gggggacgtt tgatcggggt cgtccgcgac gtcacggagc
     2641 ggaagcgaac cgagcggcgc atcgcagccc agtacgccac gacgcacatc ctcgaggagt
     2701 cgaccacgct gaaggaagca tccccacgcc tccttcggga actgtgcgag aacctggaat
     2761 gggagatggg tgcgttgtgg gaggtcgacc agcatgtgct gcgttgcgcg cgcacctggg
     2821 tagcgcctac catgcgggac tccgagttcg aggcggccac ccgaggacgg accttctcgc
     2881 gtggggaggg gttgccagga cgcgtctggg ccggcggtga gccggcctgg gtccaggatg
     2941 tgctcgaaga cgacaacttc ccacgggcgc ccctcgcggc caaggccgga ctgcacggtg
     3001 ccttcgcgat ccccatcgtg ttgcgcggtg aggtactcgg cgcactggaa ttcctgagct
     3061 acgaggtccg ccagcccgac gaaccgacac ttgcgatgat gggcagcgtc ggcatccagc
     3121 tcgggcagtt cgtcgagcgc cggcgcgcgc aggaggagtt gcgcgaggcg gaagaacgct
     3181 tcaggagcgc cttcgacgac gcggccatcg ggatggcact taacaccgtc gacggccgat
     3241 tcgtgcaggt caacgccgca ctgtgcaaga tgctaggacg ctccgaggac gagctgctgg
     3301 cgagcacctt ccaggacatt acctatccgg aagacgtgga cgcgagcgtg atgcgcgtac
     3361 gacagcttct agaaggtgag atccagagct accagctgga gaagccttac ccacacgccg
     3421 acggtcatac ggtatggatc tcgctcaacg tctctctcgt aaaagactcg aagggtgaag
     3481 cgctctacct ggtctcccag atgcaggacg tcaccgaccg caagcgcgcg gaagagagcc
     3541 tgcgggcctc cgaagccgag ctgcgggccg tgttttcggc gatgaacgac gtgatactgg
     3601 tgctcgacgc agaggggcgc tacctcgaga tcgcccccac caacccctca ctcctttaca
     3661 agccttcaga agaccttatc ggcaagacgc tgcacgagat ctttcctgca gagcaggcag
     3721 acgtgtttct ggacttcgtc aggcgagcac tgctggaaca gcggcccgtc aacctggagt
     3781 acagcctgat gatcgacggc agagagacct ggttcgcggg caccgtctcc gcgatggagg
     3841 aagacagggt cgtctacgtc gccagagaca tcacagagag caagcgggcc caggacgagc
     3901 tgcgcaagag cgaggagcga acccgcacca tcgttgagac gacccctgac gccattatca
     3961 ccatgtccaa agacggcttc atccaatcat tcaaccccgg cgcagaacgc atcttcggct
     4021 acaccaccgg ggaagccgtg ggccaaccct tgacgatgct gatgccagag agattcaagg
     4081 cgcagcacga ggccggtttc cgccgccacc tggacacggg cgaggcccac atagtcgaca
     4141 aaggcgccgt cgaattcgtg gggcgccgca aagatggcga ggagttcccc atcgagctct
     4201 ccctcggagg gatgcacgaa ggcgacgacg tcctgttcac cgggatcgtg cgcgacatca
     4261 ccgagcgcaa gaaggccgaa gaggatctca ggcagagcga ggaacgctac aggctggtag
     4321 cccaggctac cgacgagacg atctgggaca gcgacatcct ggccgacgag caggcctggg
     4381 acggggccgt cgagaccatg ttcggctact cttcggagca gcgaaccgag accacgtggt
     4441 gggaagagcg catacatcct gaagacaggg aaagggtcct atcaagcgtc gattccgctc
     4501 tcgagggcgg cgtagacatg tggtccgagg agtaccgttt catgcacgcg gatggatctt
     4561 actccaccgt cgttgacagg gcatacgtgg tgcgcgatgc cgagggcaag gctgtcagga
     4621 tgatcggctc catggccgac gtcaccgagc gccggcgggc tgagagggag ctcagggaga
     4681 acgagaagag gttcaggcag ctcttcgacc agtccgtcga cgccctcttc gtgcacgacg
     4741 cctcgggcaa gatcgtcgac tgcaacgcgg aggcgtgccg gtcgctcggt tacagcaggg
     4801 ag
//
LOCUS       DATAIR010000535         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_338980, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000535 DATAIR010000000
VERSION     DATAIR010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338980"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHM69_12685"
     CDS             <1..189
                     /locus_tag="VHM69_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741295.1"
                     /translation="PIVFDRYEGMDRPPWGNSPPRALWLGAVERALADGRMGHVLENE
                     DAHAVRPLFERVERDQEA"
     gene            240..1073
                     /locus_tag="VHM69_12690"
     CDS             240..1073
                     /locus_tag="VHM69_12690"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2741296.1"
                     /translation="MPGAEVGRERGMQMEEFSGTVAIIAGGALGIGRAAARKFADGGA
                     SVVICSNREDQVEKTVEELRGEDLEVRGTQADVTSAADMKRLVGFAAETYGGVDVLVN
                     SAGVQRYGTVVETEEDVWDEVLDVNLKGIYLASRYAIPEMWKRGGGAIVNVSSVQAFA
                     SQTGVAAYTASKGGINALTRAMALDHAPENIRVNAVCPASVDTPMLRWSANLFKGEKS
                     VDETLEDWGRMHPLGRVARPEEVAEVIVFLAGPRSSFVTGGEYKVDGGMLAALGVRLP
                     D"
     gene            1106..1981
                     /locus_tag="VHM69_12695"
     CDS             1106..1981
                     /locus_tag="VHM69_12695"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX2741297.1"
                     /translation="MEMVLDIGAASILYDGLFTEPMLDHPEGLAVHRDGSVWCGGERG
                     QVYRIEPDGSSMQQVAQAEGGFSHGMAFDREGNLYVCDLKHAAVLRLDAKRGSLETYA
                     DGAGGRGINICNYPAFDSEGRLYVSDSNAFKEPGPGIFRFDPGGSGVLWYDEPVNFAN
                     GLALSPDGGYLYVAETFGHAIFRIPIEEDGSAGRREEVARLPGALPDGLAFDAEGTLY
                     VACYEPSQVLRVVPEGTVECLIRDQEAHLLCHPTNLVFRDNVLFTTNLGRWHITSIET
                     TAEGLPLYGGYGALR"
     gene            2066..>2899
                     /locus_tag="VHM69_12700"
     CDS             2066..>2899
                     /locus_tag="VHM69_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX2741298.1"
                     /translation="MNTRDRHRLERHARRIEELRLWRNAYESPVEEWSFVAGDAGEPC
                     RIRLGDFWPEIETPVWLSAETSVPEEWSGLPVELELWLGGEGFVRLSNGTSGGLNPFH
                     RSYVVADEAEGGEKIGIEAEVVSKGLFGSHVAEPRITHAALVVPEREVRALERDLSMI
                     SEACDQLDDHHAVSHLLDACDAALISLAPYWPSESDTTVSRYLRGYENPIGNGLYSMP
                     SFAAAKALDVNRLDRDLWSKPEISPPQPLPEEARWAVRKARVLIASRLEEIKSEYPPV
                     GG"
BASE COUNT          544 a          860 c         1019 g          476 t
ORIGIN      
        1 ccgatcgtgt tcgaccgtta cgagggaatg gaccgcccgc catggggaaa cagtcctccg
       61 cgcgctttgt ggctcggtgc ggtggaaagg gccctggccg acgggaggat gggccacgtg
      121 ctggagaacg aagacgcgca cgcggtccga cccctcttcg agagggtcga gcgggaccag
      181 gaagcataga gaaaggatag agacgaggtc tccggcgcca ggcatagaga gagcgtttct
      241 tgcccggggc tgaggtgggc agggagaggg ggatgcagat ggaggaattc tccggaacgg
      301 tggcgatcat cgccggtggg gctctgggta tagggcgggc ggcggccagg aagtttgccg
      361 acggaggcgc ttccgtggtg atctgcagca accgcgagga ccaggtcgaa aagacggtcg
      421 aggaactgcg cggggaagac ctcgaagttc gaggaacaca ggctgacgtg acatcggccg
      481 ccgacatgaa gaggcttgtc ggcttcgcgg cggagactta cggtggcgtg gacgtgctgg
      541 taaacagcgc cggggtccag cgctacggga ccgtcgtcga gaccgaagaa gacgtgtggg
      601 acgaggtcct tgacgtgaat ttgaagggga tctacctggc gtccaggtac gccataccgg
      661 agatgtggaa gcgtggaggc ggggcgatag tcaacgtctc ctccgtgcag gccttcgcgt
      721 cgcagaccgg ggttgcagcc tacacggcga gcaagggagg gatcaacgcg ctcacccgcg
      781 cgatggccct ggatcacgcg ccagagaata tccgtgtgaa cgccgtctgc ccggcctcgg
      841 tggacacgcc gatgctgcga tggtcggcga acctgttcaa gggagagaag agcgtcgatg
      901 agaccctgga ggactggggc aggatgcacc ctctcggtag ggtggcccga cctgaggagg
      961 tcgccgaggt catcgtcttc ctcgccggcc caaggtcgag cttcgtcaca ggcggagaat
     1021 acaaggtcga cggtggcatg ctcgccgctc tcggcgtccg ccttccagac tgaggagccg
     1081 gcctacgaac acgggaggca tttcgatgga gatggttctg gacatcggcg cggccagcat
     1141 actctacgac gggctcttca ccgagcccat gctcgaccac ccggaggggc tggccgtgca
     1201 cagggacggg agcgtatggt gcgggggaga gcgggggcag gtctaccgca tcgagcccga
     1261 cggaagctct atgcagcagg tggcccaggc ggagggcggt ttctcccacg ggatggcctt
     1321 tgatagggag ggcaacctct acgtctgcga tctcaagcac gctgcggtac ttcggctcga
     1381 cgcgaagaga ggatctctgg agacgtacgc cgacggcgct ggtgggcgcg ggatcaacat
     1441 ctgcaattac cctgccttcg actctgaagg ccgcctttac gtctccgaca gcaacgcatt
     1501 caaggagccg ggaccgggga tcttccgctt cgaccccggt gggtcgggcg tgctctggta
     1561 cgatgagccg gtgaacttcg ccaacggact ggcgctttca ccggacggcg gctacctcta
     1621 cgtggcagag acctttggcc acgccatctt ccgcatcccc atagaggagg acggctccgc
     1681 cgggcggcgc gaggaggtcg ccagactccc aggggcgctg ccggacgggc tggccttcga
     1741 tgcggaggga accctgtacg ttgcctgtta cgagccgagc caggtgcttc gcgtcgtccc
     1801 cgaagggacg gtcgagtgcc tgatccggga ccaagaggcc cacctcctct gccaccccac
     1861 caacctggtc ttcagggaca acgtgctgtt cacgaccaac ctcgggcggt ggcacatcac
     1921 ctctatagag acgacggcgg agggcctccc gctctatgga ggttatggtg ccctgcggta
     1981 agccggttca ggccgggtgg tgtgcgaaac ggtatacaag ctgtaatatc tctcgcgtga
     2041 cttcaggctg acaagaagga gatccatgaa cacccgtgac cgtcaccgcc tcgaacgcca
     2101 cgcgcgccgc atcgaggagc tgcgtctctg gcgaaacgct tatgagagcc ccgtagaaga
     2161 atggagcttc gtggcaggtg atgctggcga gccgtgcagg atacggctcg gcgacttctg
     2221 gcccgagata gagacccccg tgtggctgtc cgccgagaca tccgttcccg aggaatggtc
     2281 tgggttgccg gtcgagctgg agttgtggct cggcggcgag gggttcgtgc ggctctccaa
     2341 cgggacgagc ggcggcctca acccgttcca ccgcagctac gttgtggcgg atgaggccga
     2401 gggcggggag aagatcggga tcgaagccga ggtagtctcg aaggggctgt tcggctccca
     2461 cgtcgcggag ccaaggatca cacacgccgc gctcgtcgtg ccagagaggg aggtgcgcgc
     2521 tctggagcgg gacctgtcca tgatctccga ggcctgcgac caactagacg accatcacgc
     2581 agtgtcccac ctgctcgacg cctgcgacgc cgccctgatc tcgctcgcgc cctactggcc
     2641 ctctgagagc gacacgaccg tatcccggta cctcaggggc tacgagaacc caatcggcaa
     2701 cggtctatac agtatgcctt cgttcgccgc cgccaaggcg ctcgacgtga accggctcga
     2761 ccgcgatctt tggagcaaac cggagatctc acctccccag ccgctcccgg aagaggccag
     2821 atgggcggtg cggaaggccc gcgttctcat tgcctcgcgg ctcgaggaga taaagtcgga
     2881 gtaccccccg gtcggggga
//
LOCUS       DATAIR010000536         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_62477, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000536 DATAIR010000000
VERSION     DATAIR010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62477"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VHM69_12705"
     CDS             <1..698
                     /locus_tag="VHM69_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741299.1"
                     /translation="DVVGHSATAVAEEISDAGGRSLAHTADVTDAGDVEAMVGAAREA
                     FGPVQILVNNAAKASDADFLDLSEETWEEDVAITLKGPFLCSQAVLPDMTENRSGVMI
                     NIASVNALTYFGNEAYSAAKAGVLSLTRSLAVRYGPSGVRVNAIAPGTLRTPAWEQRR
                     QKDPEVFERVRRWYPLGRIGEPEDVAGAALFLASDEAAWISGAVLPVDGGLTAGNLQM
                     AQEIVIGSEDNTP"
     gene            788..2002
                     /locus_tag="VHM69_12710"
     CDS             788..2002
                     /locus_tag="VHM69_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741300.1"
                     /translation="MLAFATDLHDEGLDVVLGNVQERAGLDGLTLAAAYHDARDLFPH
                     NPVSKIRYLEGGAVFFRPDESRYEDLQLRPHVSGLAQTTDPLEDLCAAADERGMDANA
                     WVVFLHSDRLGFTDPGCAARNAFGDPYLTDLCPSNPEVRGFARALASDVARYDVSTVF
                     AESLHYHGLEHGYHHERYFEEIGALGNYLLGLCFCVHCLEAAARSGVDAETVHRSVRD
                     ELERRLSGESEDEGSEEVSRERLADFGGEQLLGYLEARIATVTSLAAEVSEAVADEGA
                     RFVFMDLSGAEKGFATGQPTGEAAPTIGWRTGVDVAALAEACDTVEVTGYAADPGRLR
                     FDLDANRALLADNARPGLLLRPMPPDCRSADNLAEKVAVAREHGLERLDFYHYGFCRL
                     RSLDWIRESLTV"
BASE COUNT          362 a          665 c          664 g          344 t
ORIGIN      
        1 ccgacgtggt tggccacagc gcgacggcgg tagcagagga gatctccgat gcgggcggca
       61 ggtccctggc acacacggcg gacgtaaccg acgcgggcga tgtcgaggct atggtcggtg
      121 cagccaggga agctttcgga cctgtgcaga tactcgtcaa caacgcggcg aaagctagcg
      181 acgcggactt cctcgacctc agcgaggaga cctgggaaga agacgtggcg atcacgttga
      241 agggaccgtt cctctgctcg caggccgtgc ttcctgacat gacggagaac aggtctggcg
      301 tcatgatcaa catcgcctcg gtaaacgccc tcacgtactt cggcaacgaa gcatacagcg
      361 cggcgaaggc aggggtcctc agcctgacga ggagcctggc ggtccgctac gggccttccg
      421 gcgtgcgcgt caacgccatc gcccctggca cgctgagaac accggcctgg gaacaacgcc
      481 gtcagaagga ccccgaagtc ttcgagcgcg tgcgccgatg gtatcccctc ggccgcatcg
      541 gtgagcctga ggacgtggcc ggagccgccc tcttccttgc atctgatgag gcggcctgga
      601 tctcgggcgc cgtcctcccc gtcgatgggg gactcaccgc cggcaacctt cagatggcgc
      661 aggagatcgt catagggtcc gaggacaaca ctccatgacg cagatcccgc aggcgccggt
      721 aggcgccgcg atgcccggcc ttgatcccat acctccggct gtatagtctg gccgcatgga
      781 aacctcgatg ctcgcattcg ccaccgatct ccacgacgag ggactggacg tggtcctcgg
      841 caacgtccag gagcgggcgg gcctggacgg actgacgctc gcggccgcct accacgacgc
      901 cagggacctc ttcccccaca accccgtcag caaaattcgc tacctcgaag gcggcgccgt
      961 gttcttcagg cccgacgaat cacgctacga ggacctgcaa ctcaggccgc acgtctccgg
     1021 gctcgcccaa acgaccgatc ctctggaaga tctctgcgcg gccgccgacg agcggggaat
     1081 ggacgcgaac gcgtgggtcg tcttcctgca cagcgacagg ctcggcttca cggatccagg
     1141 ctgcgccgcg cggaacgcgt tcggggaccc ctacctcacc gacctgtgtc catccaaccc
     1201 ggaggtgcgg ggttttgcgc gggcactcgc gtcagatgtc gcgcgctacg acgtctcgac
     1261 ggtcttcgcc gagtcgctcc attatcacgg gctggagcac ggctaccacc acgagcgcta
     1321 cttcgaggag atcggggctt tgggcaacta cctgctcggt ctgtgtttct gcgtccactg
     1381 tctggaggcg gcagccaggt ccggcgtcga cgccgagacg gtccatcgct ccgtccgcga
     1441 cgagctggag cgccggttgt caggcgagag cgaggacgaa ggctccgaag aagtctcccg
     1501 cgagcgtctc gcggacttcg ggggcgagca actcctcgga tacctagaag cgcggatagc
     1561 gacggttacc tcgctcgcgg cggaggtgtc tgaggccgtc gctgatgaag gcgccaggtt
     1621 cgtcttcatg gatctctctg gggcggagaa aggcttcgcc acggggcaac ccacgggaga
     1681 ggccgcgccc acgatcgggt ggaggacggg cgtggatgtc gccgcccttg cagaggcatg
     1741 cgacaccgta gaagtcaccg gctacgccgc cgaccctgga cggctacgct tcgatctcga
     1801 cgccaatcgt gccttgctag cggacaatgc caggccgggc ctgttgcttc gtcccatgcc
     1861 ccccgactgc cgatcggcgg acaacctcgc cgagaaagta gccgttgccc gcgagcacgg
     1921 tctggagcgc ctcgatttct accattacgg gttctgtaga ctgcgctctc tggattggat
     1981 cagggagtct ctcacagtct gacaggtttc aggcttcagg tttcaggttt caggg
//
LOCUS       DATAIR010000537         7150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_35878, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000537 DATAIR010000000
VERSION     DATAIR010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35878"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..961
                     /locus_tag="VHM69_12715"
     CDS             125..961
                     /locus_tag="VHM69_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741301.1"
                     /translation="MEGALEDLFSLRGRVAVVTGATGVLGGEMARSLAHSGARVAVLG
                     RREEKARRVTEEIKQAGGEALALPADVLKRDQLEDARDALLEHWGGVDILVNAAGGNV
                     PQATLSNDVGVFDLPETALRQVMDLNFLGTLLPCQVFGAAMVEGRQEPEGCIVNVSSM
                     AASRPLTNVIGYSAAKAAVDNLTRWLAVELARSYGPGLRVNAIAPGFFIGEQNRSLLL
                     NEDGSLSQRGQTIIEHTPARRFGEPDELAGTLIWLCSRAARFVNGVVVPVDGGFSAFG
                     GV"
     gene            1066..2025
                     /locus_tag="VHM69_12720"
     CDS             1066..2025
                     /locus_tag="VHM69_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2741302.1"
                     /translation="MREFQDYTRERGGARRRWWLWSLPVVLVALFLGIGAVAGPVPLK
                     TLGYGAPPDAGRVGASDGGWIQRMLGNDGEITDRPLNVLVLGVDTRPDDEEMGSRTDT
                     IMLVQVVPGTGEVKLLSVPRDLLVEVEPGVEDRINAAYNYGGIDETIDALENYAGVPI
                     DHYAIVDFEGFSEVIGAMGGVKVDVGEDQFPEKWHLGEGIQRLGGRKALFYARYRGTP
                     GGDLDRMQRQRELVAALRSKALRWNTVRKLPETMKIMDENVQTDLGIGEAITLGRVMI
                     RQGRHAEMTSEQLKGTPETLSNGDQVLVPDEDANQPILDKFRY"
     gene            2135..2215
                     /locus_tag="VHM69_12725"
     tRNA            2135..2215
                     /locus_tag="VHM69_12725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2168..2170,aa:Leu,seq:tag)
     gene            2259..3653
                     /gene="tig"
                     /locus_tag="VHM69_12730"
     CDS             2259..3653
                     /gene="tig"
                     /locus_tag="VHM69_12730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX2741303.1"
                     /translation="MTANVTKLEENKVRLDVEVPPETVRKGVEAKVRELGRQVRVPGF
                     RPGKAPRRVIENRLGRDYIYMEALQEQLPGWYSQAVVETDLRPIDQPEIHFDDPLDEE
                     EGFKFSATVEVRPEAQLGDYKGVEVPKREVEVSDEDVEERLEELRGQFATLAAVEARS
                     AQDGDFVILDFRGERLAGGPLEGADAEDYMLEVGRGDLLPEFEENVVGMNAGERKQFG
                     VTFPMDYAEESLRGESVLFNVHVKEIKERDLPPLDDDFVKEASEFETLDELRAAVLEQ
                     LEAAAEQRIDGEFRGRVLDVVAEAAEVEIPEVMAHEKAHEMVESFERSIKAQGMEPEQ
                     YYQLAGTDHHQFEERVRPDAEDTVKKELVLDAVAAAEGIDADEDEVMHEIGHLAEESE
                     RNPEEIAETLRRNGTYSLLEEEISRQKALDFLVENAVPVPMPEEETEEPDEESVDEPV
                     EAGTTEKDEREEQE"
     gene            3650..4252
                     /locus_tag="VHM69_12735"
     CDS             3650..4252
                     /locus_tag="VHM69_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX2741304.1"
                     /translation="MSYYDPQAVIPYVIEQSPRGERAMDIYSRLLKDRIIFLGTPVDD
                     QVANAIMAQLLHLESEDPEQDINLYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMA
                     ASMGAFLLAAGAKGKRNALPNTRILLHQPSVGGLAGQASDVEIHAKELIMTKRRLNEI
                     LSQNTGQDYDKIEHDTDRDYIMGPEEGIEYGVIDNIVRNH"
     gene            4305..5564
                     /gene="clpX"
                     /locus_tag="VHM69_12740"
     CDS             4305..5564
                     /gene="clpX"
                     /locus_tag="VHM69_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX2741305.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQEDAKRVLAVAVYNHYKRIQMGAEASDG
                     TELQKSNILLVGPTGSGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNVLFVCGGAFGGLEDIIRQRIGKKSIGFGSDIDSRLKQEE
                     DSILQFALPEDLLKYGLIPEFVGRLPVISTLRSLEEADLVRILTEPKNALVRQYRQFF
                     RYDKVDLSFTEEALVAIAELALERGTGARGLRSILETTLLPTMYELPSRSDVNKCVVD
                     VDTVKDGVQPSLVTGSQKYSYDEEETA"
     gene            5572..>7150
                     /locus_tag="VHM69_12745"
     CDS             5572..>7150
                     /locus_tag="VHM69_12745"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX2741306.1"
                     /translation="MRKTEIPKTYDHRAVEGRWYEEWERRGAFEADPNPEKEPYCIVM
                     PPPNVTGALHMGHALNGSIQDTLIRRARMKGYEALWLPGVDHASIALQNVVERNLMRG
                     EGKTRFDVGREDFVERCRVFADDARSQILGQLKRLGASVDWRRLRYTMDEQYIDAVLT
                     AFVHLFDAGLIYRENRITNWCPRDRSAISDLEVNYEEVSGKLYGLRYPFVDDRGPGPD
                     GKDCVQVFSTRPETMLGDVALVVNPDDERYTELVGRRVMVPFVEREIEVLADDYVDPS
                     FGTGVLKVTPAHDPNDFEIGKRLDLKPVNVLNPDGTINENGGPFAGMSREEARKAVVQ
                     RLDEEGLLGEVRDYPHRVGHCDRCGAIIEPWLSEQWWISMKELAEPAIQALKEREITV
                     YPDSWRRESIRWLENIHDWNVSRQLWWGHRIPVWYGPEGEVVASKVSPGEGWEQDPDI
                     LDTWFSSGLWPIATLGWPARSEDLAYFYPSSLLSTAREIMYLWVARMIMMGLRFENEV
                     PFEKVNVHSIVLAEDGTK"
BASE COUNT         1489 a         2087 c         2386 g         1188 t
ORIGIN      
        1 tcatggcggg aatggtggat gaagaagaag ccagagacat ggcgcgcgag ttcgcctatg
       61 ggctcgccac aagggcctac ggattacgac gtgatcccgt cggcagtaag tgaggagggg
      121 aaggatggaa ggtgcgttag aggatctatt ctccctccgg ggaagggtcg ccgtcgttac
      181 aggcgccaca ggcgttctcg gtggagagat ggcccgctcc ctcgcgcata gcggtgccag
      241 ggtggcggtg cttggccgtc gagaggagaa ggcgcgtagg gttacagaag agattaaaca
      301 ggcaggaggg gaggcgctcg ccttgcctgc cgacgtcctt aagagggatc agctcgaaga
      361 cgccagggac gccttgctgg agcactgggg cggggttgat atcctggtca acgcggctgg
      421 cgggaacgta ccacaggcca ccctcagcaa cgatgtcggc gtcttcgatc tgccggagac
      481 ggccttgcgg caggtcatgg acctgaactt cctcgggacg ctgctcccct gccaggtgtt
      541 cggggccgct atggtcgagg gtcgtcagga gccggaggga tgcatcgtca acgtctcctc
      601 catggcggcc tccaggccgc tcacgaacgt gatcggttac tccgccgcca aggccgcggt
      661 cgataacctc acgcgctggc tcgccgtcga gctcgctcgc agctacggtc ccggtctcag
      721 ggtcaacgcc atagcgccgg gtttctttat cggggagcag aaccggtctc tactgctcaa
      781 cgaggacggg agcctctcgc agcgtggaca gaccataatc gagcacaccc cggccaggcg
      841 attcggggaa cccgatgagc tggcggggac cctgatctgg ctctgcagcc gggccgcccg
      901 cttcgtcaac ggcgtagtgg tgcccgttga cggcggcttc agtgccttcg ggggcgtctg
      961 ataggggcta tggcgcaaga gtaatagatc ggggcgcagg tcgctgccgg cgagtgtcgg
     1021 agatttgttt gctttgcagg taggggcgga ttagactccc tgtggatgag ggagttccag
     1081 gattacacaa gggaacgtgg aggtgcgcgc cgccgctggt ggctgtggtc gttgcctgta
     1141 gtactggtcg cgttgttcct gggcataggg gccgtcgccg gcccggttcc gttgaagacg
     1201 ctgggctacg gtgcgccgcc cgacgccggg agggtcgggg catctgatgg cggttggatc
     1261 cagaggatgc tcggtaacga tggtgagatc acggaccgac ccctcaacgt gctcgtcctc
     1321 ggcgtggata cgcggcctga cgatgaggag atggggagcc gcacggacac cattatgctc
     1381 gtgcaggtcg tgccagggac tggagaggtc aagcttctct cggtaccgag ggatctgctt
     1441 gtcgaggtgg aacctggggt cgaggacagg atcaacgcgg cctacaatta cggcggcatc
     1501 gatgagacga tcgacgcgct ggagaactac gccggcgtcc cgatagacca ctacgcgatc
     1561 gtggacttcg agggcttctc cgaggtcata ggcgccatgg gtggggtgaa ggtagatgtg
     1621 ggagaagatc agttccccga gaagtggcac ctgggtgagg gcatccagag gctcggcggg
     1681 cggaaggctc tcttctatgc caggtacaga ggtacgccgg ggggagacct ggaccgcatg
     1741 cagcgccagc gggagctcgt ggcagccctt cgcagcaagg cgctcagatg gaatacagtc
     1801 aggaaactgc ccgagaccat gaagatcatg gacgagaacg tccagacaga tcttggcatc
     1861 ggcgaggcca tcacgctcgg tcgtgtcatg atccgccagg gaaggcacgc cgagatgacg
     1921 tcggagcaac tcaagggcac gcccgagacg ctctccaacg gagaccaggt cctcgtcccc
     1981 gacgaggatg ccaaccagcc gatcctggac aagttccgct actagggtac tctttgagcc
     2041 accggaccgg gcttgcgccc gtagcgtaaa gcgggtccat gcgtccgggt ggagattgga
     2101 cggctgctat aatccggcgc aggtcctacg gcgggcgaga gtggtggaat ggtagacacg
     2161 ctagatttag gatctagtgc cttcgggcgt gcgggttcaa atcccgcctc tcgcacatcg
     2221 ccgcccaacg tcgggccggt aaagaggaac tggagggtat gacggctaac gtaacgaaac
     2281 tcgaagagaa caaggtcagg ctcgatgtcg aggtgcctcc tgagacggtc aggaagggcg
     2341 tcgaggcgaa ggtcagggag cttggccgtc aggtgcgggt ccctggtttc cgccctggca
     2401 aggcgccgcg gcgggtgata gagaaccggc tcggacgcga ctatatctac atggaggccc
     2461 ttcaggagca actccccggc tggtattcgc aggcggtggt ggagaccgac ctgcgcccga
     2521 tcgaccagcc cgagatccac ttcgacgacc cactggatga ggaggagggc ttcaagttct
     2581 cggccaccgt cgaggtgcgc ccggaggcgc agctcggcga ctacaagggt gtcgaggtcc
     2641 cgaagcgcga ggtcgaggtc tcggatgagg acgtcgagga gcggctggag gagttgcgag
     2701 gccagttcgc aaccctggcc gccgtcgagg caaggtccgc gcaagatggc gacttcgtga
     2761 tcctggactt caggggcgag aggcttgccg gtgggccgct cgagggcgcc gacgccgagg
     2821 actacatgct cgaggtcggg cgcggggacc tgctgcccga gttcgaggag aacgtcgttg
     2881 ggatgaacgc cggtgagcga aagcagttcg gcgtaacttt cccgatggac tacgccgagg
     2941 agtccttgag gggcgagtcg gtgctcttca acgtgcacgt caaggagatc aaagagcgag
     3001 acttgcctcc cctcgacgac gattttgtca aggaggcgag cgagttcgag acgctggacg
     3061 aactgcgcgc cgccgtgctc gagcagcttg aggccgccgc cgagcagcgc atcgacggcg
     3121 agttccgtgg gcgtgtgctc gacgtcgtgg ccgaggctgc cgaggtcgaa atccccgagg
     3181 tcatggctca cgagaaggct cacgagatgg tcgagagctt cgagcggagc atcaaggcgc
     3241 aggggatgga gcccgagcag tactaccagc tcgccggcac ggaccatcac cagttcgagg
     3301 agcgcgtcag gcccgacgcc gaggacaccg tcaagaaaga gctcgtgctc gacgccgtcg
     3361 ccgcggcaga gggcatagac gctgacgaag acgaggtgat gcacgagatc gggcacctcg
     3421 cagaagagtc cgagaggaac cccgaggaga tagcagagac cctgcgccgc aacgggacct
     3481 actcgctgct ggaagaggag atctctcgcc agaaggccct cgatttcctg gtagagaacg
     3541 cggtacccgt gccgatgcct gaggaagaga cggaagaacc cgacgaagaa tcggttgacg
     3601 aacccgtaga ggccggtacg acggagaagg acgaacgaga ggaacaggaa tgagctacta
     3661 cgatccgcaa gctgtaatcc cctacgtcat agagcagagc ccgcgggggg agcgggcaat
     3721 ggacatctac tcgaggctgc tcaaggaccg catcatcttc cttggcactc cggtggatga
     3781 ccaggtggcg aacgccataa tggcgcagct attgcacctg gagagcgagg accccgagca
     3841 ggacatcaac ctatacataa actcccctgg cggaagcgtc acggcggggc tcgcgatcta
     3901 cgacaccatg cagttcgtca agcccgacat ctccacgacc gccctcggca tggcggcctc
     3961 gatgggggct ttcctgctcg cggcgggcgc caagggcaag aggaacgcgc ttcccaacac
     4021 ccggattctc ctgcatcagc cgagtgtcgg cgggctggcg ggccaggcat ccgatgtgga
     4081 gatacacgcc aaagagctga ttatgaccaa acgccgccta aacgagatcc tctcccagaa
     4141 cacggggcag gactacgaca agatcgagca tgacaccgac cgcgactaca taatgggtcc
     4201 cgaagagggg atcgagtacg gcgtcatcga caacatcgtc aggaaccatt aggggttcct
     4261 ggagccattg gaatcgatag agatgtcggg gaggatgtag tggcatgaga gatccgtcgg
     4321 atcagttgca gtgctctttc tgcggcaaga gccagcgtca ggtcaggaag ctcatagccg
     4381 gcccaggcgt ctatatctgc gacgagtgca tcgagctgtg caacgaaatc atcgacgagg
     4441 agttctccgg gcccgaggtc ctgaaggatg atgatcttcc aaaaccacgt gagatcaacc
     4501 gcatcctcaa cgagtacgtt ataggccagg aggacgcaaa gcgtgtcctc gccgtcgccg
     4561 tctataacca ttacaagcga atccagatgg gcgccgaagc gtccgacggc accgaactcc
     4621 agaagtccaa tatattgcta gtcggaccga cgggttcagg taaaacgctc ctcgcccaga
     4681 cgctcgccaa gatactcaac gtccccttcg ccatagcgga cgccacggcc ctgaccgagg
     4741 caggctacgt cggcgaggac gtcgagaaca tcctcctcaa gctcatccag gcggccgact
     4801 tcgacgtgaa gaaggccgag accgggatca tctacataga cgagatcgac aaggtcgccc
     4861 gcaaggcgga taacccttcg atcacccgcg acgtctcggg cgagggcgtg cagcaagccc
     4921 tcctgaagat cctcgagggc acggtcgcct cggtcccgcc tcagggcggt cgcaagcacc
     4981 cgcaccagga cttcctccag atagacacca agaacgtcct gttcgtctgc ggcggagcgt
     5041 tcgggggact cgaagacatc atccgccagc gcatcggcaa gaagagcatc ggcttcggga
     5101 gcgatataga ctcgcgcctc aagcaggagg aagactccat cctgcagttc gcattacctg
     5161 aggatctctt gaagtacggc ctgatccccg agttcgtcgg gcgtctgcct gtgatctcga
     5221 ccctccgcag cctggaggag gctgacctcg tgcgtatcct caccgagccg aagaatgcgc
     5281 tcgtacgcca gtacaggcag ttcttccgct acgacaaggt ggaccttagt ttcactgagg
     5341 aggccctcgt cgcgatcgcc gagctcgccc ttgagcgcgg gacaggggcc cgcggcctga
     5401 ggagcatcct ggagactact ctcttgccga cgatgtacga gttgcccagc cgctcggacg
     5461 tgaacaagtg cgtcgtggat gtagatacgg tgaaggacgg cgttcagccg tcacttgtaa
     5521 ctggctccca gaagtactcc tacgacgaag aagagaccgc ttaaagaccc cttgaggaaa
     5581 acagagatac cgaaaaccta cgaccaccgc gccgtcgagg gccgctggta cgaagagtgg
     5641 gagcgccgcg gggcgttcga ggcagacccg aaccccgaga aggagcccta ctgcatcgtc
     5701 atgccgccgc ccaacgtaac gggcgcgctg cacatggggc acgccctcaa cggctccatc
     5761 caggacaccc tgatccggcg cgccaggatg aagggctacg aagccctctg gctccccgga
     5821 gtcgaccacg cctccatcgc cctccagaac gtcgtcgagc ggaacctgat gcgcggggaa
     5881 ggaaagacgc gcttcgatgt gggccgcgag gatttcgtcg agcggtgccg cgtcttcgcc
     5941 gacgacgccc gctctcaaat actcgggcag ctcaagcgcc tcggagcttc ggtggactgg
     6001 cgcaggctcc gttacacgat ggacgaacag tacatagacg ccgttctcac ggctttcgtt
     6061 cacctgttcg acgcggggct catctacaga gaaaaccgta taacgaactg gtgcccgcgc
     6121 gaccgctccg ccataagcga tctcgaggtc aactacgagg aggtttctgg aaagctatac
     6181 ggcctgcgct accccttcgt ggacgacagg ggccccggtc ccgacggcaa agactgcgta
     6241 caggtcttct ccacccgccc cgagacgatg ctcggggacg tcgccctggt cgtcaacccg
     6301 gacgacgaac gctacacgga gctggtggga cgccgcgtga tggtgccatt cgtagagcgc
     6361 gagatcgagg ttctcgccga cgactacgtc gacccctcct tcgggacggg agtcctcaag
     6421 gtcacgccgg cacacgaccc gaacgacttc gaaataggga agaggctgga tctcaaaccc
     6481 gtcaacgtcc tcaaccccga cggtaccatc aacgagaacg gcggcccttt cgccgggatg
     6541 agccgcgagg aggcaaggaa ggccgtcgtc cagcgtctcg acgaggaggg tttgctcgga
     6601 gaggtacggg attatccgca cagggtcggg cattgcgaca ggtgcggggc catcatagag
     6661 ccctggctct cggagcagtg gtggatctcc atgaaggagc tcgccgagcc agcgattcag
     6721 gcgttgaagg aaagggagat caccgtctac cccgattcct ggcgccgcga gagtatccgg
     6781 tggctggaga acatccacga ctggaacgtg tcacgccagc tgtggtgggg gcaccgcatc
     6841 cccgtctggt acggtcctga aggggaggtc gtggcctcca aggtgtcgcc cggcgaggga
     6901 tgggagcagg accccgacat cctcgatacg tggttttcga gcggcctgtg gcccatagcg
     6961 acgctcggct ggcccgcaag gtcagaggat cttgcatact tctacccgtc gagcctactc
     7021 tcgacggcgc gtgagatcat gtacctgtgg gtggcgcgta tgatcatgat gggactgcgc
     7081 ttcgagaacg aggtgccttt tgaaaaggta aacgtccact ccatcgtcct tgccgaggac
     7141 gggacgaaga
//
LOCUS       DATAIR010000538         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_36165, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000538 DATAIR010000000
VERSION     DATAIR010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36165"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHM69_12750"
     CDS             complement(<1..432)
                     /locus_tag="VHM69_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741307.1"
                     /translation="MGARRPQQNRTVAVALVFALCLLMLISVSDHARAAVGNFVPRQV
                     IVELRQPGASIEEINVAYGSSTLLRYSDSSDVYLLKLPTGSGVLDTVDRMTSDGRLLY
                     SEPNYVAEPPEGGARHKAWGVSSDAPTSQNYAATALNLSAAH"
     gene            748..1104
                     /locus_tag="VHM69_12755"
     CDS             748..1104
                     /locus_tag="VHM69_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741308.1"
                     /translation="MERLLLVEANLLFRDGLALLLEWRTGLSSVYAGSLAEAERFLED
                     PGQKPACVIVDLDLPDGDGTELLKQLNGLPVLALIKGRSLERRAQAIKSGADEVVRTT
                     QPPEKIIAAVERLIAH"
     gene            1201..1536
                     /locus_tag="VHM69_12760"
     CDS             1201..1536
                     /locus_tag="VHM69_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741309.1"
                     /translation="MALEDDYRTYRDVIAAVIQISRPKVETASVGLDELEEKVRTFNP
                     HLVICSLPATADLERVPGWVELSLDLSRPTKISIKGRHVVQSTRMGLEQLLTVISEVE
                     QIAPKDHGP"
     regulatory      1644..1722
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1854..2630
                     /locus_tag="VHM69_12765"
     CDS             1854..2630
                     /locus_tag="VHM69_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741310.1"
                     /translation="MDKTILARKHALFSGAIVVLALTLAALLVALVAARPSEAAESIT
                     IDPSNIGFGAVTLSADAQTRTVTITNNGLAAITIGSIDYSGLPGTEIGTFETLIGPGG
                     LLVGAGQTATLDVDFDPVTTGFKQATGTIKDLVGVEIVGAPQLVVSGTGVTTQPAGVP
                     SDCTIVGTNSGERLTGTSGKDVICALGGADRVFGRGSGDVERGGSGNDLISDKMGRDK
                     LLGQGGKDRLSARDGGRDILKGGKRSDRCARDKRDRARSC"
     gene            2925..3467
                     /locus_tag="VHM69_12770"
     CDS             2925..3467
                     /locus_tag="VHM69_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741311.1"
                     /translation="MSLTRYYTIGAVAQDLRTLKALDERLEDLDVQADSLIVFSRRAD
                     ERLVSVTLPGARTRSVESGLSRTQWFEFASTYLGVMAVSVLMGAVHPPTGMAVQAIMT
                     LAAIVGLVLYHRTSHLEKKLLAMGLPERFADEWKDAFSQGFALALVSVPEDLFDETQD
                     AFLEDAGLRSPLAVDRRPVL"
     gene            3595..4290
                     /locus_tag="VHM69_12775"
     CDS             3595..4290
                     /locus_tag="VHM69_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX2741312.1"
                     /translation="MIVRDRPDSFLLFEQHEHALIAGEFARRWEERPSPFEPTVYAVA
                     NHDLAWREPDREVLWNEEKDRPFAFVDYPMELKLPAQKKGIDLVEDHDSYAGCLCSMH
                     YARFLLDSQRPEEVEFREGEIERQSRLREGMGGEELENLERNFHFLRLCDGLSLFLCL
                     NEPGSGASPPPYPGGFTFEGTRFKPVWEDDRTLRLDPNPFSEAFGIQIPYQMVGSNRR
                     HLGSDILDLRITC"
BASE COUNT          943 a         1312 c         1382 g          837 t
ORIGIN      
        1 gtgggcggcc gagagattga gcgccgtggc cgcgtaattc tgggaagtcg gcgcatcgga
       61 gctcacaccc catgctttgt gcctggcgcc tccctcgggc ggctcggcca cataattcgg
      121 ctcggagtag agcaatcgcc cgtctgacgt catccggtct accgtgtcca atacgccaga
      181 tccggtcggc aacttgagta gatagacgtc gctgctatcc gaatatctca gcagcgtgct
      241 cgatccgtag gcgacgttga tctcctcgat gctggcacca ggctgacgta actcgactat
      301 gacctggcgg ggcacgaagt tccccacagc ggcccgtgcg tggtcggaaa cgcttatgag
      361 catcagcaag cacagcgcaa atacgagcgc tactgctaca gtacgatttt gctgcggcct
      421 cctggcgccc aaagtccccg cctcacacta gtgtcactgg ccgcattata catctacttc
      481 tgtcggcccc gcgatatttt gcgtcgaact caggcggttc acatttgtca taaaaatgag
      541 acttcgggta tttgccgacc ctctaagccg cataactatc atgccatgac aaacgtgaac
      601 cggcggcgag gggtggtgtc tgaggcagag gacgtgttcc tgcctggctt cgcctccggt
      661 tttggacctc gaagaacgtg tctctgaggt ggttgcagga aagataggaa gtggtcgcac
      721 atacatgatc tccaaggtgg taccgtgatg gaacggctat tgcttgtcga ggccaacctt
      781 ctatttcggg acggcctcgc gctactgctc gaatggcgaa cgggcttgag tagcgtttat
      841 gcagggtcgc tcgccgaggc ggagcgtttt ctggaggacc ccggccagaa acctgcctgc
      901 gttattgtcg acctcgatct gccggacggc gacgggaccg aactactcaa gcaattgaac
      961 gggctgccgg tgctggcgct catcaaaggc cgcagcctgg aacgccgagc ccaggcgata
     1021 aaatcagggg cggacgaggt ggtacgcacg acacagccgc ccgagaagat aatcgccgca
     1081 gtggaacggc tcatcgccca ctagatcgct ggttcacacg tagcgcagcc cgccctgctg
     1141 ccgtaagata cacgcccagg ggaacggcat aggcagcagg ggggcgcaat acgcgtacta
     1201 gtggctctgg aggacgacta ccgcacctac cgggatgtta tcgctgccgt catccagatt
     1261 tcgcgcccga aggttgagac agcgagtgtc ggactggatg agcttgaaga gaaggtgcgg
     1321 accttcaatc cgcacctggt gatctgcagc ttacccgcta cggcagatct tgaaagggta
     1381 cccggctggg tagagctttc cctggatctg agtcgcccga cgaaaatttc catcaagggg
     1441 cgccacgtgg tgcagagcac ccgtatgggg cttgaacaac tgctgaccgt catatcggaa
     1501 gtcgagcaga ttgcgccaaa agatcatggt ccttaacttg ccccgatgac gcacgcgaga
     1561 acctattcac actccgatgt attttcggac cctgtccgcg gcatatcagt gtcatagggc
     1621 atcccaggaa atgaggatgg ccatcctgca aaaggcaaac ctgtcgtgag gcgggggcgc
     1681 aaagccacgg acctcctgga aggaggtagc cgggttgccg aaaggaggtt ctacagcact
     1741 taacttcccg ctcacaccgg cgaggcaaga cagacatcgt caggtcggaa aaggagcttc
     1801 ggagtgtaaa gggcaccccg atgcaacatc catagaattg gagacactca tccatggaca
     1861 aaaccattct agcgcgcaag cacgcgctct tcagcggggc catagtagtg ctcgcactta
     1921 ccctcgcagc gcttctcgtg gccctcgtgg ccgccagacc ctctgaggcc gcggagtcga
     1981 taaccatcga cccgagcaat atcggcttcg gcgccgtcac gctcagcgcc gacgcccaaa
     2041 ccaggaccgt gaccataacc aacaatggcc tggctgctat tacgatcgga agcatcgatt
     2101 attctggtct ccctggcacg gaaatcggca ccttcgagac tttgataggc ccaggcggcc
     2161 tactcgtagg agccggtcag actgccaccc tggacgtcga tttcgaccct gtgacgacag
     2221 gattcaagca ggcgaccggg acgatcaagg acctggtagg agtcgagata gtgggagcgc
     2281 cgcaactcgt ggtgagcggc accggggtga ctacccaacc cgccggggtg ccatcggact
     2341 gcacgatcgt cggcaccaac agcggcgagc gtttgaccgg cacctcgggc aaggacgtca
     2401 tctgcgcttt gggcggagcc gaccgcgtct tcgggagagg ctccggcgac gttgagcggg
     2461 gtggatccgg caacgacctt atcagcgaca agatgggccg cgacaaactg cttggccagg
     2521 gtggcaagga tcgcctcagt gccagggacg gcggccgcga catcctcaag ggaggaaaac
     2581 gtagcgaccg gtgcgccagg gacaagaggg acagggcaag gagttgctag cctagggccg
     2641 caactgtgag agccgggacc gaatgctccc ggctctctat gccatctggc gcaggtactc
     2701 ggaacctcga caagtcaact cccggcacac gctcacccgg ccgggagtgc tgtcatggct
     2761 ggtgtatctt tgctaacggt ccaccgccag gagtggcgct agactctatc ggcagtagaa
     2821 tacgaggacc agccaggagt gagcgtgcga tccatgtcaa gacatctccc tgtggcccgt
     2881 tcggggcaca gtcccagccc gagatgccag caggttggta cgccatgagc ctgacacgct
     2941 actacacaat cggggccgtc gcgcaggacc tgcggacgct caaggcgtta gacgagcggc
     3001 tggaggacct ggacgtacag gccgactcgc tcatcgtgtt ctcgcggcgc gcggacgagc
     3061 gcctcgtgag cgtaaccctt ccgggtgcca ggacgcgcag cgtcgagtcg gggctctcgc
     3121 gcacgcagtg gttcgagttc gcgagcacat acctcggggt aatggccgtg agtgtcctaa
     3181 tgggcgccgt ccatccacct accggtatgg ccgtgcaggc aattatgacg ctggccgcca
     3241 tcgtcggcct cgtcctctac caccgcacct cgcacctgga aaagaaactg ctcgccatgg
     3301 gcctccccga gaggttcgca gatgagtgga aagatgcctt ctcacaaggc ttcgccctcg
     3361 ccctcgtcag cgtacccgaa gacctcttcg acgagacgca ggatgccttc ctcgaagatg
     3421 ccggtctgag atctccgttg gctgtagaca ggcgaccagt tctctaggtt tcaggcctca
     3481 ggctacaggc ttcaggtctt gctgccgatc tccgtctcat ggccgtgaac ctggtgttaa
     3541 gcgcaactgg ctgaagcccg atgccttatc tcgaaacctg ataccttatt gcgtatgatc
     3601 gttcgtgaca ggcccgactc tttcttgctc ttcgagcagc atgaacacgc cctcatcgca
     3661 ggcgagtttg cgcgccgatg ggaggagagg ccgtcgcctt tcgagccaac ggtctacgcg
     3721 gtagccaacc acgacctcgc ctggagggag cctgacaggg aggtgctgtg gaacgaggag
     3781 aaggatagac ccttcgcatt cgtcgattac ccgatggagc tcaagctgcc ggcacagaag
     3841 aaggggatag atctcgtgga agatcacgac tcctatgcgg gatgcctgtg cagcatgcac
     3901 tacgcccgat tcttgcttga ctcacagagg cccgaggagg tcgagttccg cgagggtgag
     3961 atagagcgcc agagccgact cagggagggg atgggcggag aggaactgga gaacctggag
     4021 cgcaacttcc acttcctgag gctgtgcgac gggctctcgc tgtttctgtg cctgaacgag
     4081 ccaggaagtg gcgcaagccc gccgccgtat ccgggagggt tcacgttcga gggtacacgc
     4141 ttcaagcccg tgtgggagga cgataggacg ttgcgcttgg atcccaaccc gttctctgag
     4201 gcttttggga tccagattcc ctaccagatg gtcggtagca accggcgaca cctcgggagc
     4261 gatatactcg atcttcggat aacctgttag gggcgccgga acggcgcact tttagaaacc
     4321 catttccacc acagaaagga ctcatatgag ggacgcgtcg tacgatttct tgaagaggct
     4381 gctctcgacg cctggaccga gcggctacga ggaggccgcc gcgaagatct ggagggagga
     4441 ggcagggaag ttcgccgaag tctacggtga cagg
//
LOCUS       DATAIR010000539         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_129244, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000539 DATAIR010000000
VERSION     DATAIR010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129244"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHM69_12780"
     CDS             complement(<1..367)
                     /locus_tag="VHM69_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741313.1"
                     /translation="MESLDKLETQGGQMRLSANAGSLSILFSQEVWEDLISNAPAFEE
                     PTPEELEELDLMKHQLERYARQRSFSTVHRGVRCGSSGLDYDLVLRPAGLFAMTTGYH
                     FTPLVGPDLVAKVGVALEGD"
     gene            733..1374
                     /locus_tag="VHM69_12785"
     CDS             733..1374
                     /locus_tag="VHM69_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX2741314.1"
                     /translation="MSASDSNLRSRLREWFGRLGRAQKVLAASATLVITVGGVASGIT
                     AVLDLGGRISAGSPASSDTPATSLPVAEGVRVTEVIPDSPADRAGLEVGDIITDVGGE
                     SVEDVDDLRKVLSTFGDSEAVRMSVVTDSGKRSVLVKPEPIEGTRQRMGIGAQDLFPK
                     DGAFRKSSRLDEESRARSAQDAGETPAGARGLEGESATDSSDAPSARQYPAED"
     gene            1765..2301
                     /locus_tag="VHM69_12790"
     CDS             1765..2301
                     /locus_tag="VHM69_12790"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX2741315.1"
                     /translation="MTTNEHKRREVQEILGVELRRADLDLPEIQAIDPADVAAEKVRA
                     AREALGSPGLPVLVEDSGLMVDAWDGFPGALTKWIMQSVGNEGLLRMLGPDNDRSAMA
                     VCVVALAEADGTVRTFRGEVPGTLAPEPRGEGGFGYDPVFVPGWSSLTYAEMGDDKNT
                     DSHRARAFREVRRWLGEA"
     gene            2409..>3145
                     /locus_tag="VHM69_12795"
     CDS             2409..>3145
                     /locus_tag="VHM69_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2741316.1"
                     /translation="MSADNEHSENGGVETREDGGAIATSSERLVDLYKEMALIRAFEE
                     ACQRGFRQGKIGGYLHVYIGQEAVATGFLQAFKEGDKVITAYRDHAHALLLGSDPNEV
                     MAELYGKGTGLVKGKGGSMHLFDVERGLMGGYGIVGGHIPPGVGFAYAMKYQETDHIT
                     QLYLGDGAISTGAFHEAANLAGLWGKDGMCPCLFIVENNQYGMGTSVERTTAMTDLAA
                     KFDAYGIENEKVDGMDIEAVIEVAERTA"
BASE COUNT          653 a          886 c         1041 g          565 t
ORIGIN      
        1 tgtcgccttc gagcgcgacc ccgaccttcg ccaccagatc ggggccgacg agcggcgtga
       61 agtggtagcc tgtcgtcatg gcgaagaggc ccgccggacg cagcacgaga tcgtaatcca
      121 gacccgaact cccgcacctc acacctctgt gcacggtgct gaaggatcgt tgtctcgcgt
      181 accgttcgag ctgatgcttc atgaggtcca gctcttccag ctcctcagga gtcggttctt
      241 cgaaggccgg cgcgttggag atgaggtcct cccagacctc ctgcgaaaag agaatgctca
      301 gggaacccgc gttggccgag agacgcatct ggcctccctg tgtctccagc ttatcgaggc
      361 tctccaccct cacctcatgc atggacgcga ctgtatcata aagaaccata aagcgtacat
      421 actctggcat tattctcgtg agtctggtca gatcttcagc ctggctactt caccgtgaca
      481 cggtcggaat aggaagcgtc gccccgctgc tccctcacat aaacttccac ggcattctct
      541 cttggcggca atttgactca ccccagcatc cagttcatcg tgaattcatc ggcgattcat
      601 cgtcctctgc ttctatgctg tgggcgttgc gcgaacgtgg cgcaatagcc atagaaggaa
      661 gctcacaagc acaggtggtg ttccaacgtt gggtcgagaa acgggctctc ggcaggttag
      721 aggtagagtc tcatgagcgc cagcgacagt aatctgcgat cccgccttcg ggagtggttc
      781 ggcagactgg gccgcgccca gaaagtgctg gccgcgtcgg cgaccctggt aataacggta
      841 ggcggggtcg cgtcaggtat cacagcggtg ctcgacctgg gaggtcgcat cagcgccggc
      901 agcccggcgt caagtgacac cccggcaacg tctctacccg tggcggaggg agtgcgcgtg
      961 acggaggtaa ttccggatag tccggcggac cgtgccggat tggaggtcgg ggatatcatc
     1021 acggacgtcg gtggcgagag cgtggaagac gtggacgacc tccggaaggt actgagtaca
     1081 tttggcgaca gcgaagccgt gcgcatgtcc gtggtcacgg attcaggcaa gagatccgta
     1141 cttgtgaagc ctgaacccat agagggcacg cgtcagcgta tgggaatcgg agcacaagac
     1201 ctgttcccga aagatggagc cttccggaaa tcaagtcgct tggacgagga atctcgggcg
     1261 cggtcggccc aggacgccgg tgagaccccc gctggggcca gggggctgga aggtgaatcc
     1321 gcaacggatt cgtcggacgc tccctccgcc agacagtatc ccgccgagga ctgaccggct
     1381 ccctgacgct atcgggaggg ccttcaatgt actcggcaac agctaccgca tcaaccccgt
     1441 ccggagcctt gctgtagcta ccatcagatg tagcctgcga ttgagcacac ttcgatactc
     1501 aagcgaatcc ggaagacggg atggccttga agactctcgg agcgagagta gctccaacgt
     1561 ggcaacgtac cccaggaacg ggagagcagg agaagaagtg tgcggctgga gcaggcgata
     1621 gaatcgtcct gcggcctatc gactgacagg cttggcgaaa gtagtaagct tcgcagacga
     1681 atagcagcct cgattcgtgc cacattgcgt acgttttatg tagactgttt gaatttgcag
     1741 gggagagcca tcagagcaat cttcgtgacc acaaacgagc acaagcgccg cgaagtacag
     1801 gagatcctcg gcgtcgaact gaggcgggcg gatctggacc tgcccgagat acaggcgatc
     1861 gaccccgccg atgtagcagc cgagaaggtc cgcgcggccc gcgaagcgct tggcagcccg
     1921 gggcttcctg tcttagtcga ggactccggg ctcatggtag acgcgtggga cgggtttcca
     1981 ggggcgctga ccaagtggat catgcagagt gtcggtaacg agggactgct caggatgctc
     2041 ggacctgaca atgaccgctc ggccatggcc gtgtgcgtcg tcgcgctcgc ggaggcggac
     2101 gggacggtac gcaccttcag gggtgaggtt ccgggaacac ttgcgcctga gccgagggga
     2161 gagggcgggt tcggctacga tcccgtgttc gtccccgggt ggtcctctct cacctacgcc
     2221 gagatgggcg atgacaagaa caccgactct cacagggcgc gggcgttccg ggaagtgcgc
     2281 cggtggttag gagaagcatg aaaatggggt ataagggcct atgggatagg ttgagggatt
     2341 acgtacgaaa ctcgcggttt tggtattctg tccctgggtt tgtacctctg agagggaagg
     2401 ggccgtcaat gtcggcagat aacgagcact cggaaaacgg gggcgtagag acccgtgagg
     2461 acggtggcgc catagcgacc agttcggagc gcctcgtaga cctgtacaaa gagatggccc
     2521 tgatccgggc cttcgaggag gcgtgccaga ggggcttcag gcagggtaag atcgggggct
     2581 acctccacgt ctacatcggg caggaggccg tagccacggg cttccttcag gcattcaagg
     2641 aaggcgacaa ggtcatcaca gcctaccgcg accacgcgca cgcgctactt ctggggagcg
     2701 atcccaacga ggtcatggcc gagctatacg gcaaggggac gggcctggtg aagggcaagg
     2761 gcggctcgat gcatctcttc gacgtggagc gcggcctgat gggcggctac ggcatcgtcg
     2821 gcgggcacat accgcctggg gtgggtttcg cctacgcgat gaagtatcag gagacggacc
     2881 acatcaccca gctctacctg ggtgacggtg cgatcagcac cggcgccttc cacgaggccg
     2941 cgaacctcgc cgggctctgg gggaaggacg gcatgtgccc ctgcctgttc atcgtcgaga
     3001 ataaccagta cgggatgggc acgtcggtgg agcgaacgac ggcgatgacc gatcttgcag
     3061 ccaagttcga cgcctacggc atcgagaacg agaaggtcga cggcatggac atcgaggcgg
     3121 tcatcgaggt cgcggagagg acggc
//
LOCUS       DATAIR010000540         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_36307, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000540 DATAIR010000000
VERSION     DATAIR010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36307"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VHM69_12800"
     CDS             complement(<1..518)
                     /locus_tag="VHM69_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741317.1"
                     /translation="MLGRYAELKAQLPPGTILFYQVGTFFETFEEDAKKISQALSIRL
                     TSREAAGEGRVPLAGVPGHAIQEHLATLLRKGYSVAVAEQRQHPTKPKQFTREVTRVL
                     TPGTVIEDNVLSAGHANYLAAFVVRDGRAGIAVVEASTGEFTGTEVPEEGLAAELERW
                     SPREIVVPGRTAA"
     gene            complement(494..1066)
                     /locus_tag="VHM69_12805"
     CDS             complement(494..1066)
                     /locus_tag="VHM69_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2741318.1"
                     /translation="MRFNHTVETEASPEEIWTNINNWPAWDTELASATLDGEFAKGTS
                     GRLRPGKRPTSTFFISDLTQGESYAFTTRLPLCELEVRRSLVRTGDGGTYFTHEVSFA
                     GPLSFAFGRILGGRYQESPPAVMENPRKIAEAVRRNGPSRQMLLAQSDVGHGAASASL
                     FRAFLSIKYSGLTVEVLRRRINPCSDDTRS"
     gene            complement(1077..1460)
                     /locus_tag="VHM69_12810"
     CDS             complement(1077..1460)
                     /locus_tag="VHM69_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2741319.1"
                     /translation="MCDVIFGAVNVMPFRRWRRALRPGGTTVTVNPIFVNGALRWLAR
                     TVGGARLGSVQPSGADLETIGGWISTGQILPVVDRRYMLAEAHAYSESKRVRGKLVLV
                     VRVAEKADSTQREADRDPVIDGSAS"
     gene            complement(1499..>2683)
                     /locus_tag="VHM69_12815"
     CDS             complement(1499..>2683)
                     /locus_tag="VHM69_12815"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX2741320.1"
                     /translation="ERIQTIDALRGFALFGILVVNMAAFRAPVFDETGLDAGVLDRVV
                     TWLIAFGFEQKFYVLFSFLFGYGLSVQMFRAAERGSPLVPRFLRRLLGLLLIGLAHAV
                     LLYTGDILVTYALLGVVLLLMRNVSSRVLLLLAAGLVLLSALAFALSGTVFATGDNAA
                     FEAELRAESERSVEAYRGTPAAAISERIRTYPGTLGFALVGQGPTALAMFLVGLWAGR
                     RRLFERVEDHLPLLRRALVVGLVVGVAGALVWASERAVSGFAFDEGFFLAASVDFLTA
                     PFLTAAYVVAITFLYRSPGWRRRLAFLVPVGRAALSNYLFQSLVAALIFTGYGLGLYG
                     RVDAGLGLLLSFAIFAAQIPLSAWWMSRFAFGPAEWVLRSFTYARLQPIRVRLKVPQT
                     PP"
BASE COUNT          540 a          883 c          849 g          411 t
ORIGIN      
        1 gccgccgtac gcccggggac gacgatctcg cgcggcgacc acctctccag ctcggcggcg
       61 agcccttcct cgggaacctc agtgcccgtg aactcgcctg tcgaggcctc gacgaccgct
      121 ataccagccc gaccgtccct gaccacgaac gcggccaggt agttggcgtg tcctgcggag
      181 aggacgttgt cctctataac ggtgccaggc gtcagaacac gggtgacctc acgcgtgaac
      241 tgcttcggtt tcgtcggatg ctgccgctgc tcggcgacgg cgacggagta tcccttcctg
      301 agaagagtag ccaggtgctc ctggatggcg tgaccgggga cacctgcgag cgggacgcgg
      361 ccctcgcccg ccgcctcgcg gctcgtcagc cggatcgaga gcgcctgtga gatcttcttc
      421 gcgtcctcct cgaaggtctc gaagaacgta cccacctggt agaagaggat ggtcccaggc
      481 ggcagttgcg cttttaactc cgcgtatcgt ccgagcatgg atttattcta cggcgcaaca
      541 cctccaccgt cagccccgag tatttgatgg acaagaaggc gcgaaacaga gaggcggatg
      601 ctgcaccgtg gccgacatct gattgtgcga gcagcatctg gcgtgatggc ccgttacggc
      661 ggacggcttc tgcgatcttc cgcgggttct ccatcaccgc cggcggagac tcctggtaac
      721 gccctcccaa tattcttccg aacgcgaagg atagcggccc ggcgaaggag acctcgtggg
      781 taaagtacgt tccaccgtcg cccgtgcgca ccaaagagcg tctgacctcg agctcgcaca
      841 gaggcaatcg ggtcgtgaac gcgtaactct ctccctgggt aaggtcggag atgaagaacg
      901 tggaagttgg tctcttccct ggtctcagcc taccactcgt ccctttcgcg aattcgccat
      961 ccagtgtggc ggaggcgagc tcggtatccc aggcgggcca attattgatg ttggtccaga
     1021 tctcctccgg cgaggcttcg gtttcgacgg tgtggttgaa cctcatgtcg tctccttcat
     1081 gatgcggaac catcgatgac aggatcacgg tcagcttcgc gctgcgtcga gtccgccttc
     1141 tcggcaacac ggacgacgag cacgagcttg cctcgaaccc gtttggattc gctatacgcg
     1201 tgagcctcgg cgagcatgta gcgccggtcc acgacaggaa gtatctgccc ggtcgagatc
     1261 caacccccaa tcgtctccag gtccgcgccg ctcggctgga cagaccccaa cctggcgccg
     1321 ccgacggtgc gcgccaacca tcgcagtgcg ccgttgacga atatggggtt gaccgtgacg
     1381 gtggtgcctc cagggcgcag cgcccgacgc cagcgccgaa agggcatgac gttgaccgcg
     1441 ccgaagatca catcgcacat cggtcctccc tcccgcgtca cgaaggtctt tctctatgct
     1501 atggcggcgt ctgaggcacc ttgaggcgaa cccggatcgg ctggaggcgg gcataggtga
     1561 acgagcgcag cacccactcg gccgggccga aggcgaagcg ggacatccac caggcgctca
     1621 gggggatctg ggcggcgaag attgcgaagc tcaagagcag cccgagcccg gcgtcgaccc
     1681 gaccgtagag cccgaggccg tatccggtga agatcagcgc ggcgacgagc gactggaaca
     1741 ggtagttgct gagcgccgcg cgccccaccg gcaccagaaa cgccagacgg cgccgccagc
     1801 ccggtgaccg gtagagaaac gtgatcgcga ccacgtacgc cgcggtgagg aagggcgcgg
     1861 tgaggaaatc caccgacgca gcgaggaaga acccctcatc gaaagcgaat cccgagacgg
     1921 cccgctcgct ggcccagaca agagctccgg cgacgccgac gacgagcccc accaccagcg
     1981 cccgccgcaa cagaggcaga tgatcttcca cgcgttcgaa gagcctgcgg cgccccgccc
     2041 agagcccgac caggaacata gcaagcgccg tcggcccctg gccgacaagg gcgaatccca
     2101 gggtacccgg atacgtcctg atccgctctg agatcgccgc tgcaggcgtg ccacggtagg
     2161 cctcgacact acgctcgctc tcggcacgaa gctcggcctc gaacgcggcg ttgtccccgg
     2221 tggcgaatac cgtgccgctg agtgcgaaag ccagggccga gagcaacacg aggccggcag
     2281 ccaagaggag caggaccctg gaggacacgt tcctcataag cagcaggacg accccgagca
     2341 acgcgtaggt aacaaggata tcgcctgtgt agagcagcac ggcgtgggcc agccctataa
     2401 gcagcagccc gagcaatcgg cgcaggaaac ggggaactaa gggcgacccg cgttccgccg
     2461 cccggaacat ctgcacgctc aacccgtagc cgaacaggaa cgagaacagc acgtagaact
     2521 tctgctcgaa gccgaaggcg atcaaccacg tgacgacgcg atcgaggacg ccggcgtcga
     2581 ggcccgtctc gtcgaaaaca ggggccctga acgcggccat gttgacgacg agaatcccga
     2641 agagcgcgaa cccacgcagc gcgtcgatcg tctggatgcg ctc
//
LOCUS       DATAIR010000541         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_62719, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000541 DATAIR010000000
VERSION     DATAIR010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62719"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VHM69_12820"
     CDS             <1..789
                     /locus_tag="VHM69_12820"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2741321.1"
                     /translation="TFQSTGTVNAIYRLGDHLCVRLPRVQRWAQDLEKEWHWLPKLAP
                     SLPLRVPEQVGKGHPVSSYPFSWAIYGWIEGQPYSDELVDDEHQAARDLAHFVAELRR
                     IDPVIGAPRGGRSPLRDLDAVTREAIESARGVIDGDAATAAWERALEAPAWKGIPTWI
                     HTDLLRPNVLVHGGRLCAVIDFGGIGVGDPAADVIAAWSVFSHTGRGTFRGALDVDDG
                     AWERARGFALHQAAMIIPYYGETNPGFVSLAKRTVEEILADIDT"
     gene            complement(739..975)
                     /locus_tag="VHM69_12825"
     CDS             complement(739..975)
                     /locus_tag="VHM69_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2741322.1"
                     /translation="MLLQLPPEFTVEGMGVLEDFKKSLPEGFRYAVEVRHRSWLGSDL
                     PELLRECGVADEARLPERSRVDISEDFLDGALRQ"
     gene            1103..1468
                     /locus_tag="VHM69_12830"
     CDS             1103..1468
                     /locus_tag="VHM69_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741323.1"
                     /translation="MPSVRSGMSIGIMFMLILSSLVIVLSAPRADAQAVCQTELDALE
                     SAITSATFTNEKDRQNLLAKLSNAEAKLTEGKTNDAVAKVTDIQSAAARLAEGNKLDP
                     DGAKAIDAAAAVAIGCLQP"
     gene            complement(1635..2438)
                     /locus_tag="VHM69_12835"
     CDS             complement(1635..2438)
                     /locus_tag="VHM69_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2741324.1"
                     /translation="MDDSAQQRLFAETPGSIEPPERGLYLGTSGWSYADWEGTLYPEA
                     LPSGSRLAEYVKRFATVEIDSTFYGTPRRPTVQKWREIAPDGFLFAAKFPQEITHEKY
                     LVGSETEAESFVRTMAELGDRLGPLLLQLPPSFDVEGMGVLEDFLKSLPEGFRYAVEV
                     RHRSWLGSDLPEMLRERGAALTLLDYPRMPRMQEATAEFSYIRWLGDRREFPEGHTHL
                     KKERDEDLRWWSHLVDRFLQEDMTVFAYANNHYQNHSPSTLERFLELRR"
BASE COUNT          536 a          813 c          850 g          526 t
ORIGIN      
        1 acgtttcagt cgacggggac ggtgaacgcc atctaccggc ttggcgacca cctctgcgtc
       61 cgactgccgc gcgtgcagag gtgggcacag gacctggaga aagagtggca ctggcttccg
      121 aagctcgctc caagtctgcc actgagagtc cccgagcagg ttgggaaggg tcaccctgtg
      181 agctcgtacc ccttctcttg ggcgatctac ggatggatcg aaggccagcc gtattcggat
      241 gagctcgttg acgatgaaca ccaggcggcc agggaccttg cgcacttcgt ggccgagctg
      301 cgccgaatag atcccgtaat cggggcacct cgcggcgggc gcagtccact ccgcgatctc
      361 gacgcagtca cgcgcgaggc gatcgagtcg gcgcggggcg tcatagacgg cgatgcggcc
      421 accgccgcct gggagcgagc actcgaagca ccggcgtgga aaggaatacc cacgtggata
      481 cacaccgacc tgctgaggcc caacgtgctc gttcacggcg gccgactgtg cgcggtcata
      541 gacttcggcg gtatcggggt cggtgatccc gccgctgacg tgatcgccgc gtggagcgtg
      601 ttcagccata ccgggcgagg aacgtttcgc ggtgccctcg acgttgacga cggcgcctgg
      661 gagcgagcac gtggcttcgc actccaccag gctgccatga tcatcccgta ctacggcgag
      721 acgaacccag ggttcgtctc actggcgaag cgcaccgtcg aggaaatcct cgctgatatc
      781 gacacgtgac ctctcgggca agcgcgcttc gtccgcgacg ccgcactcac gcagcaactc
      841 ggggagatcc gagcccagcc agctcctgtg acgcacctcg acggcgtagc gaaatccctc
      901 gggcagactc ttcttgaagt cttcgaggac ccccatccct tcgacggtga attccggcgg
      961 aagctgaagt agcacagacc gaagagcagc ttttcgcttg gatagtcaat ccagtagcct
     1021 cggttttgcg tatatactgc acgtgggcag cgaatatcgg ctgtcccttg caatgctcac
     1081 tataggaagg agcgcataag taatgccaag cgtacgctca ggtatgagca tagggatcat
     1141 gttcatgcta attctctcct cgctagtgat agttttgtcc gccccaaggg ctgatgcaca
     1201 ggcggtttgc cagacggagc tggatgccct ggagagcgct attacgtcgg cgaccttcac
     1261 taatgagaaa gaccggcaga acctgctcgc caagcttagc aacgcggaag ccaagcttac
     1321 tgagggcaag accaatgacg ccgtagccaa ggttacagac atccagagtg cggcagcgag
     1381 gcttgctgaa ggaaacaagc tggacccgga tggtgccaaa gccatcgatg ccgcagccgc
     1441 tgtcgcgata gggtgcctgc aaccctaatc tgacacgtag gctgcgagtt accccagtct
     1501 aacaacgggg agcgcctctt tccccgttgt tagactgaga ctctatacat gttgtggtgc
     1561 tggatggatt tgcagttgga tgaagaccgg gggccaggag agtgatctcc ttcaggacac
     1621 cttgggcggc tcatctatcg cctcaactcc agaaatcgct ccagcgtgga tggcgagtgg
     1681 ttctggtagt ggttgttggc gtaggcgaag acggtcatat cctcctgaag aaagcggtcc
     1741 acaagatgcg accaccacct gaggtcctcg tcacgctcct tcttgagatg ggtgtgtccc
     1801 tcagggaact cccgtctgtc accgagccag cggatgtagg agaactcagc cgtcgcctcc
     1861 tgcatgcggg gcatcctggg atagtcgaga agcgtcaggg cagcgccgcg ctcgcgtagc
     1921 atctcgggga ggtccgaacc cagccaactc ctgtgacgca cctcgacggc gtagcggaag
     1981 ccttcgggca gactcttcag gaagtcttcg aggacgccca tgccctcgac atcgaaggac
     2041 ggcgggagct gaaggaggag cgggccgagc ctgtctccca gttcggccat cgtgcgcacg
     2101 aagctctccg cctcggtttc tgagccgacg aggtactttt cgtgtgtgat ctcctgcggg
     2161 aacttggccg cgaagaggaa gccatcgggt gcaatttcac gccacttctg cacggtcggg
     2221 cgacgcgggg tgccgtagaa ggtcgagtct atttcgacgg tcgcaaatcg cttgacgtac
     2281 tcggcgagcc gcgaccctga agggagcgct tctggataga gtgttccctc ccagtcggcg
     2341 tacgaccagc ccgaggtccc gaggtacagg cccctttcag gcggctcgat gctgcctggt
     2401 gtctccgcga agagccgctg ctgtgccgaa tcgtccattc gcttacaagc taccatactg
     2461 gccctcgagg gttctatgaa cgcgcttcct gtttgccttc gccgcgtgta ctacattatt
     2521 gcggtggacg aacgtcacca gacagcgtga aggtcttcta cagtgatctg ttcgtgctcc
     2581 ccttgccgga ggggcaccgc ttcccgatgg tcaagtactc gatgctgcgc gagcgcgtcg
     2641 cggacgatgg ggtctgcggt cccggtgaac ttcaggtccc acgcgcggta acggacgagg
     2701 agattctgcg ggctcacgaa ccaaa
//
LOCUS       DATAIR010000542         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_36479, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000542 DATAIR010000000
VERSION     DATAIR010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36479"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /gene="eno"
                     /locus_tag="VHM69_12840"
     CDS             <1..316
                     /gene="eno"
                     /locus_tag="VHM69_12840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2741325.1"
                     /translation="GIKTSTANAILVKVNQIGTLSETFAAVDLAQRNGYAAVMSHRSG
                     ETEDATISDLAVAVNAGQIKTGAPARGERVAKYNQLLRIEEALGTSAFYPGRAAFNPR
                     RG"
     gene            319..1743
                     /gene="pyk"
                     /locus_tag="VHM69_12845"
     CDS             319..1743
                     /gene="pyk"
                     /locus_tag="VHM69_12845"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2741326.1"
                     /translation="MRRTKIVATLGPATSSEEGIGDLVRAGVDVTRFNLSHGEHGVHL
                     ENAGIVRGVAKELGRTVAILQDLQGPKIRTGEVESDTELVEGSRVVIAPGDFVGDASR
                     LATSYEDLPNDVRPGHRLLIDDGLIGLRVMSIEDGEVVCEVIEGGPVSSHKGLNFPDS
                     TLSIRGLTPKDVDDLRFGMEELHPDWVAMSFVRTADEVMEVKERIREFGGNAPVISKI
                     EKHEGIDNIEEIIAASDGIMVARGDLAVELSAERVPIEQKRMVARCRRLGKPVIVATQ
                     MLDSMIRNPRPTRAEVSDVANAIFDRADAVMLSGETAVGRYPMQSVQEMERICRAAEG
                     AINYGRDIPASASWGRGDRYDAVTHAACELAEDLQAEAILTSTQTGLSCIRVARFRPP
                     NKILAISPIEHTVRMLALVWGVISIHGEQAGSLEDRFREAIEAARKAGHLKDGDEVIL
                     TGGTAGSVPGSTNVVEVATVGEDR"
     gene            1765..2046
                     /locus_tag="VHM69_12850"
     CDS             1765..2046
                     /locus_tag="VHM69_12850"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX2741327.1"
                     /translation="MSIPRRLRPLVVVFYAAFIGLLLASYVPPLQDILHNRAEIPVLE
                     QKLQKARTQNTANERLIRELNTPAGIERAARERYGMIRPGEKVYIIPKE"
     gene            2159..2599
                     /locus_tag="VHM69_12855"
     CDS             2159..2599
                     /locus_tag="VHM69_12855"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX2741328.1"
                     /translation="MPETSKAVVARQLGREPRPFRVAARCPFGLPSVIENERTREMPT
                     SFWITCPSLVAAISRIESTGGVREAQNELGEATIEEIHTDHRSRYGTRVAGVREGGNV
                     KCLHAFTALHLSGAIPNPVAEWTIQRLENPYPEDHCCSAENVET"
     gene            3097..3180
                     /locus_tag="VHM69_12860"
     tRNA            3097..3180
                     /locus_tag="VHM69_12860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3131..3133,aa:Leu,seq:taa)
BASE COUNT          706 a         1001 c         1075 g          550 t
ORIGIN      
        1 ggggatcaag accagcacgg ccaacgcgat cctcgtcaag gtcaaccaga ttggcacgct
       61 gagcgaaacc ttcgccgccg tggacctggc gcagcgcaac ggttacgcgg cggtgatgtc
      121 gcatcgcagc ggcgagacgg aagacgcgac gatatcggac ctcgccgtgg cagtgaacgc
      181 aggccagatc aagaccggcg ccccggcccg cggagagcgg gtcgccaagt acaaccagct
      241 actccgcata gaggaggcgc tgggaacctc ggctttctat cccggccggg ctgccttcaa
      301 cccgaggagg ggctgacgtt gcgcaggaca aagatagtcg cgactctggg gcctgccacc
      361 tcctctgagg agggtatagg ggacctcgtc cgcgcgggtg tggacgttac caggttcaac
      421 ctctcgcatg gcgagcacgg cgtgcacctc gagaacgccg ggatcgtgcg cggcgtggcg
      481 aaagagctgg gacgcaccgt cgccatactg caggacctcc agggacccaa gatccggacc
      541 ggcgaggtcg agagcgatac ggagctggtc gagggcagcc gggtcgtgat cgcgcccggg
      601 gacttcgttg gtgacgcgag ccgtctcgct acctcctacg aagacctccc caacgacgtg
      661 cgcccgggcc accggctgct catagacgac ggacttatcg ggctgcgggt aatgagcatc
      721 gaggacggcg aggtggtctg cgaggtgatc gaaggcggcc ctgtctcgtc ccacaagggt
      781 ctcaacttcc ccgactcgac cctctcgata aggggcctca cgccaaagga cgtcgatgac
      841 ctgcgcttcg ggatggagga gctccacccc gactgggtgg ccatgtcctt cgtgcgcacg
      901 gcggatgagg taatggaggt caaggaacga ataagggagt tcggcggcaa cgcgcccgtc
      961 atctccaaga tcgagaagca cgagggcata gacaacatcg aggagatcat agccgcctcg
     1021 gacggcatca tggtcgcgcg gggggacctt gccgtggagc tctctgccga gagggttccg
     1081 atagagcaga agcgcatggt ggcgcgatgc aggcgcctcg ggaagcctgt catcgtcgcc
     1141 acccagatgc tcgactcgat gataaggaat ccccgcccga cgcgggccga ggtctcggac
     1201 gtggccaacg ccatcttcga tcgggccgac gccgtcatgc tctcaggcga gacggccgtc
     1261 gggcgctacc cgatgcagag cgtgcaggag atggagcgga tctgcagggc cgcggaggga
     1321 gctatcaatt acgggcggga catccctgcc tcggcctcat gggggagggg agaccgctac
     1381 gacgccgtca cccacgccgc ctgcgagctc gccgaagacc tgcaggccga ggcgattctg
     1441 acctctaccc agacggggct ctcgtgcatc agggtcgccc gcttcaggcc gccgaacaag
     1501 atccttgcca taagccccat cgagcatacc gtcaggatgc tcgcgctggt gtggggtgtg
     1561 atctccatcc acggggaaca ggctggttct cttgaagacc gtttcagaga ggctatcgag
     1621 gccgcccgga aggccggaca cctcaaggac ggcgacgagg tcatcctgac cggtgggacc
     1681 gccgggtctg tgcctggctc cacgaacgtc gtcgaggtgg ccacggtagg agaagaccgt
     1741 tagaaggagc gtggcggaac cgaggtgagc ataccccgga gactgaggcc gcttgtggtc
     1801 gtgttctatg cggccttcat cggcctcctc ctcgcctcct acgtccctcc tctgcaggac
     1861 attctgcata accgtgccga gatccccgtc ctcgaacaga agctacaaaa ggccaggacg
     1921 cagaataccg cgaacgaacg ccttataagg gagttgaaca cccccgccgg catcgagcgg
     1981 gccgcccgcg aacgctacgg gatgatccga cccggcgaga aggtgtacat cattccgaag
     2041 gaataggcat cagccgtcag gaaccgcatg aggactaacc acgccacgac aacgcaaccg
     2101 gccatgaccg ggtttcgcct tgctgcagga gcgccgctgg ccaagaatct gatgcctgat
     2161 gcctgaaacc tcgaaagccg tcgtcgcccg acagctcgga cgcgagccgc ggcctttccg
     2221 cgtcgccgcc cgctgtccct tcggccttcc gagcgtcata gagaacgagc ggacgcgaga
     2281 gatgccgacg agcttctgga tcacctgccc gagtctcgtc gccgccatct cgcgtatcga
     2341 atcgacaggc ggcgttcgcg aagcgcaaaa cgagcttggt gaagcaacga tagaggagat
     2401 ccacaccgat caccgctcgc gctacgggac ccgtgtcgct ggtgtccgcg aggggggtaa
     2461 cgtcaagtgc ctccacgcct tcacggccct ccacctctcg ggcgccatcc ctaaccctgt
     2521 cgcagaatgg accatccaac gcctcgaaaa tccctacccg gaggaccact gctgcagcgc
     2581 ggagaacgtg gaaacctagc tgttatactc ccgacctgag cccgggggcg tagcccaatc
     2641 ggcagaggca gaggaccttt aggccaggga attcgggtaa tgaggttatg gatgcgtggt
     2701 ccatataaag acagaaaatc ggagtatgtc agggcagtcg agctgcgtag atccggttac
     2761 ggttacagaa gcatcgctaa cgagatagac gttccctggc gtacagtttg tggttgggta
     2821 aagcacattc gggtcgaccg acgcgtagct tacgataatg caattgcacg caagaagcga
     2881 aaggcattcc ccgtcgggaa gagcgcgatt cgcttgaggc taatggagga gcgtggtagc
     2941 gtttgccagg cgtgcgggtt agaagaatgg ttcggtgagc cgatagtgtt ggaaatgcac
     3001 agaaaggagg caggtggaga ctacacgcca gacaatatcc ttctcctctg tcccaattgt
     3061 catagcatta ccaatacctg gaggaacaag aagcgcgggg gcgtggcgga atcggcagac
     3121 gcaccggact taaaatccgg gtcctgaaaa ggagtgtggg ttcgagtccc accgccccta
     3181 ctcatataac agcggcttgg ggaaggattg atcctcccca agccgggcta caggcaacag
     3241 gtttcaggca tcaggaaggt cttccctgac tgacggtggt cgctaaggtg caccttcggg
     3301 gcgaagtgga tcaatactta tctactgcgc tg
//
LOCUS       DATAIR010000543        10111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_451886, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000543 DATAIR010000000
VERSION     DATAIR010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451886"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VHM69_12865"
     CDS             complement(<1..1122)
                     /locus_tag="VHM69_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741329.1"
                     /translation="MRARAARLAPLVFAASLFFSVAALIVVTLNGDRIGSADATGSAI
                     LAVSYSAVGVMIVVRRPGNPIGWILLLGGFCNGLNAFSWEYARYALITTAGSWPLGPF
                     FAWLSTWIFAPGFVASFPLTLLLFPTGRPPSPRWRPLLWLIVIGLALAVLPMAVAAWP
                     IRGPDLVTSFLWESEALGDLAVTLQRAGVGVIILCILASVVSVGFRYWSAGGEERQQL
                     KWLVYAGALTFVVTVTASPSALFELSGFAGVILSILAPLALPSIPVAVGIAILRHRLY
                     DIDIVINRTLVYGSLTATLAAVYVVCVVVLQYLFRVLIGGELQLAVVASTLLIAALFG
                     PLRRRVQASIDRRFYRRKYDAAKTLEQFGGRLRDEVDLDT"
     gene            complement(1119..2357)
                     /locus_tag="VHM69_12870"
     CDS             complement(1119..2357)
                     /locus_tag="VHM69_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741330.1"
                     /translation="MNPRVASRLAWSLWGFALGFAAAGLLFGVLSFSAELPEGREPFL
                     ASIVVQDVLVVLYGVLGALIASNQGRNVIGWIFCFVAVSLGVISFAYGFADYALYARE
                     NPLPGAVLAAWVPNWLFIPAVYVSMCYLFFLFPDGRPASPWWRPVIWAATIVAAVATL
                     ATALEPGRLYSFPTVENPFGLGEPLGRVAIVANDVTDLLAMLVFLVSLASMIARLRRA
                     RGRERQQLKWITYSAALTATSFATAFLAGSLAQDRRVFADAFFLLGVVGFTSIPVAAG
                     IAILRHRLYDIDIVINRTLVYASLTATLAAVYVVCVVSLQFIFRVITGQESQLAVVAS
                     TLAIAALFNPLRRRIQILVDRRFYRRKYDAKRVLDEFAARLRDETDLEVLGDDIIAVS
                     RETMQPAHVSLWLRKAEGER"
     gene            complement(2354..3595)
                     /locus_tag="VHM69_12875"
     CDS             complement(2354..3595)
                     /locus_tag="VHM69_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741331.1"
                     /translation="MDHKKIVFWLAWSLCLLCVGLALCAPLLWLLNGRTLIGFVREGD
                     CAVVVLVISFSVVGALIVSHRPENTIGWIFCAAALFQALSEVGLEYATYALVTRPGSL
                     PFGAGMSWLAEWVWAPGLGLILVFLPLLFPDGRPPSRRWRPVAWLGGLSIGLISVPVA
                     VLTLSENGKALLSDGGDGEEGLGRLVWIVEAGFPLMLLAGLLAVVSLFIRFRGASGDE
                     RQQIKWFAFAAALTFAWIFVFEQLVSAEGGALEAIAAVSSLVLVPSIPVATGIAIFRY
                     RLFEIDRLVNRTLVFAALTAALVLVYLGGVISLQYAFRAITGQESNLAVVASTLAIAA
                     LFGPLRRRIQAFIDRRFYRRKYDAAKTLEGFSGKLREEVELDSLTDDLVAVVRETVQP
                     THVSLWLRSPVEALRSPGRDR"
     gene            3869..4711
                     /locus_tag="VHM69_12880"
     CDS             3869..4711
                     /locus_tag="VHM69_12880"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX2741332.1"
                     /translation="MREIAASTITEAVRDLCIEANTRLPQEHLAALRRALGAEESELG
                     REVIERLLENAEVARERCVAFCQDTGYAVFFVEMGSGVRISSGEIGASIDEGVRQGYE
                     EGYLRKSIVRSPIDRTNTGDNTPAVVYYEPVPGDGLKLTMLVKGAGCDNMSALKMLTP
                     AEGLPAMKSFVVETIERAGPNASPPLTVGVGIGGPFEKAAQLAKKALTRPSGEPNPDP
                     ELAGLERELLEEINATGIGPAGYGGTQTALAVHVESFPTHIAAFPVAVNLDCHSHRTA
                     SIEL"
     gene            4708..5265
                     /locus_tag="VHM69_12885"
     CDS             4708..5265
                     /locus_tag="VHM69_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX2741333.1"
                     /translation="MTPEPIYLHAPLSIEDIEPLRTGDVVRLNGVLYTARDAAHARMK
                     EALEAGEPLPFDPEGQVVYFTGPAPARPGHALGPAGPTTASRMDPYSPLLIERGLRGM
                     VGKGSRSEAVLDSMREHGCVYFGAVEGTAALLADCVKEAEVIAYEDLGAEAIRRLVVE
                     DFPVVVVNDLYGGDLYREGKERWRR"
     gene            complement(5262..6644)
                     /locus_tag="VHM69_12890"
     CDS             complement(5262..6644)
                     /locus_tag="VHM69_12890"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX2741334.1"
                     /translation="MATRKIQNERVQNLNEADVRDGDHVLYWMQSSQRAEQNHALEYA
                     VQRANDLGQRLLVVFGLTDDYPEANLRHYTFMLEGLEDVQEALKERGIKMVVRKGSPD
                     AVALDAGKDASLIVTDRGYLRPQRRWREKVAGEAGCLVTQVESNVVVPVELASEKRET
                     AARTLRPKIQEYLEDFLVGLTPMKIEKQSLNVRAEGLDLSDIERVLDGMDLDRSVGPL
                     SHLYRGGTSEAKKVFRIFLKDSFSNYDGHRNQPQTDDVSHMSKYLHFGHVSPIWLALE
                     ARQARTKKANIESFVEELIVRRELSMNFVHYTPDYDSYSCLPDWARKTLEEHKDDERE
                     HTYTRKHLESAQTHDEYWNTAMKEMVHTGYMHNYMRMYWGKKILEWSNTPEHAYRTTL
                     YLNNKYFLDGRDPNSFTNVAWIFGQHDRGWTEREVFGKVRYMSAGGLERKTKPEQYVE
                     KIEKRIDASG"
     gene            complement(6774..7190)
                     /gene="msrB"
                     /locus_tag="VHM69_12895"
     CDS             complement(6774..7190)
                     /gene="msrB"
                     /locus_tag="VHM69_12895"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2741335.1"
                     /translation="MTDTPRKIQKTEEEWQDQLTSEQYRITRMKGTERPFSGEYYENK
                     EEGVYRCVACGTPLFSSDTKYESGTGWPSFWAPLDESNVETEEDRSLFMRRIEVHCAT
                     CGAHQGHVFDDGPAPTGQRYCINSAALRFDPEAKPE"
     gene            complement(7228..8376)
                     /locus_tag="VHM69_12900"
     CDS             complement(7228..8376)
                     /locus_tag="VHM69_12900"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEX2741336.1"
                     /translation="MSLRGKAAVVGVAESDLGEVGAGFTPLDLIGQATLRALDDAGLQ
                     KSDVDGLFSASPYYHMPTLSVGEYLGIRPRYSDATSMGGSSFVSHLFHAAAAIDAGLC
                     EVALITYGSTQRSGGGRLVTGSEADPYEAPYKPRYPVSMYALAASRHMHEYGTTREQL
                     AEVAVAAREWAKLNPKAFMRDDLTVEDVLDSRMVSSPLTVRDCCLVTDGGGAAIVTSA
                     ERARDFIERPVYLLGAGEAHWHRNMSQMPDLTVSAAAESGPRAYEMAGVGPEDIDVAM
                     LYDAFTINTLLFLEDLGFCEKGEGGTFVSGGRISPGGELAVNTSGGGLSYNHPGMYGL
                     LLIVEAVRQLRGECGERQVEVANIALVHGNGGVLSSQVTAILGSESTL"
     gene            complement(8373..8750)
                     /locus_tag="VHM69_12905"
     CDS             complement(8373..8750)
                     /locus_tag="VHM69_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX2741337.1"
                     /translation="MSQEEGGPPAEIYRGYLESGELGFQRCAGCGAAVFYPRVLCSVC
                     GGADLVWETSSGLGVVYATTAVYRRDADPYNVVLVDLEEGFRMMSRVKGMPAEEVEVG
                     TRVALRIDREGDVPVPVFVPEDR"
     gene            complement(8871..>10111)
                     /locus_tag="VHM69_12910"
     CDS             complement(8871..>10111)
                     /locus_tag="VHM69_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741338.1"
                     /translation="EADAFTSKFQQYTGPVMAKGMEDAALYVYNRLISLNEVGGEPER
                     FGVSVPEFHRLNAERLECWPHSMLSTSTHDTKRSEDVRARIDVLTEIPGEWRESVVRW
                     SELNAPHRRDAGGELAPSRNDEYLLYQTLLGAWPLGELDGKEQEAFRKRVKEYMEKAM
                     REAQVRTSWTDANEKYEDAVAGFVDALLSESNPFLREFLPFQRRVARIGALNSLSQSL
                     IKLTAPGIPDVYQGNEIWDFSLVDPDNRRPVDFGLRKRLLAHLRELDPSDVRMLLDEG
                     AWQDGRPKLYLIWKALRMRSELPELFGDGGYVALHTSGKRREHIVAFARLHGGEVALT
                     VAPRLLAKMMDVEGSLIPTPEAWEDTSILLPAELAEGTYRNVLTGEKVTTEEHNGQQS
                     LHVGRLLHNFPVALLETGDR"
BASE COUNT         1942 a         3193 c         3182 g         1794 t
ORIGIN      
        1 cgtgtccaga tccacctcgt ccctcaacct gccaccgaat tgctccagcg tcttcgccgc
       61 gtcatatttc ctgcggtaga agcggcggtc tatggaagcc tggaccctgc ggcgcaaagg
      121 tccgaagagg gctgcgatca agagcgtcga agcgaccacc gccagctgca actctccccc
      181 gatcaggacg cggaagagat actgcagcac gaccacgcag acgacgtaga ccgcagcgag
      241 cgtcgcggtg agcgaaccat agaccagcgt cctgttgata acgatgtcta tgtcgtagag
      301 gcggtgcctg agaatcgcga tgccgacggc gacgggtatg ctgggcaggg cgaggggggc
      361 caggatcgag aggatcaccc cggcaaaccc ggacagctcg aataacgccg agggcgacgc
      421 cgtgacggtc acgacgaacg tgagagcccc ggcgtagacc agccacttga gttgctggcg
      481 ttcctcgcct ccggcactcc agtaccggaa gcctacggag accacggagg cgagtatgca
      541 caggatgatg acccccaccc ccgcgcgctg tagcgtcacc gccaggtcac caagggcttc
      601 gctctcccac aggaagctgg taaccaagtc cggacccctg atcggccacg ccgcgacggc
      661 catcggcaac acggccagcg cgaggccaat gacaatcagc cagagcagtg gacgccagcg
      721 cggagatggc ggacgtcccg tcgggaagag cagaagcgtg agggggaagg aggccacaaa
      781 ccctggggcg aaaatccagg tcgagagcca cgcgaagaaa ggccccaaag gccacgaacc
      841 ggcggtcgtt atcagggcgt acctggcata ctcccaggag aaggcgttaa gaccgttgca
      901 gaagcccccg aggaggagga tccaacctat cggatttccg ggccgccgga cgacgatcat
      961 cacgcccacc gcggagtagg agacggcgag gatcgcggag cccgtcgcgt ccgccgagcc
     1021 gatccggtct ccgttcaggg tcacgactat caaggccgcg accgagaaga ataaggacgc
     1081 ggcgaagacg agcggggcga gccgggcggc gcgtgccctc atcgctcgcc ttccgccttt
     1141 cttagccaca gcgacacgtg ggccggttgc atcgtctccc gcgacaccgc gatgatatcg
     1201 tcacccagga cttcgaggtc cgtctcatcg cgcagcctcg cggcgaactc gtccagtact
     1261 ctcttcgcgt cgtacttgcg cctgtagaag cgacggtcca cgagaatctg gatgcgccgc
     1321 cgcaagggat tgaacaacgc cgctatcgca agagttgagg cgacgaccgc gagttgcgac
     1381 tcctgcccgg ttatgacgcg gaagatgaat tgcagggaga ccacgcagac gacgtaaacc
     1441 gcagcgagcg tcgcggtgag cgatgcgtag accagcgtcc tgttgataac gatgtctatg
     1501 tcgtagaggc ggtgcctgag gatcgctatg ccggcggcga cgggtatgct ggtgaagccg
     1561 accacgccca gcaagaagaa cgcgtccgcg aagacccgcc ggtcctgcgc gagggagccg
     1621 gccaggaacg cggtggcgaa gctcgtcgcc gtgagcgccg cggagtaggt gatccacttg
     1681 agttgttggc gctcccggcc ccgtgcgcgt cgcaggcgtg cgatcatcga ggccagcgag
     1741 acgaggaaga cgagcatcgc gaggaggtcc gtcacgtcgt tggcgacgat ggcgactctg
     1801 cctaagggtt ctcccaggcc gaacgggttc tccacggtcg ggaatgagta tagccgtccg
     1861 ggctcgaggg cagtggcaag ggtcgcgacc gcggcaacga tggtcgcggc ccagatcacc
     1921 ggccgccacc acggagaggc cggacgcccg tcgggaaaga ggaagaacag gtagcacatc
     1981 gagacgtaca ccgcgggtat gaacaaccaa ttcggcaccc aggccgcgag cacagcgcca
     2041 ggcagggggt tctctctcgc gtaaagggcg tagtctgcga agccgtaggc gaaggagatg
     2101 acgccgagcg agaccgccac gaagcagaag atccagccga tcacgttccg cccctgattg
     2161 gaggcgatca acgctcccag aaccccgtag aggacgacca gcacgtcctg aacaacgatg
     2221 gacgccagga agggttccct cccctcaggt agctccgccg aaaacgagag tacgccgaat
     2281 aagagaccag cggcggcgaa gccaagcgcg aacccccaga gcgaccaggc gagccgggag
     2341 gcaacgcgcg ggttcatcgg tcccgccccg gcgaccttaa cgcttcgaca ggggaacgca
     2401 gccacaagct gacgtgggta ggctgaaccg tctcccttac cacagccacc agatcgtcgg
     2461 taaggctatc aagctcgacc tcctcgcgca gtttgccaga gaagccttct agcgtcttcg
     2521 ccgcgtcgta tttgcggcgg tagaaacggc ggtctatgaa agcctggatg cggcgccgca
     2581 gcgggccgaa cagcgccgcg atagcgagcg tggaagccac cacggccagg ttagactctt
     2641 gcccggtgat agcacggaag gcgtactgca aggagatcac accccccagg tagaccagga
     2701 ctagtgccgc cgtgagggcg gcgaagacga gggtgcgatt gactagccga tctatctcga
     2761 agagccggta gcggaagatg gcgatacccg tggcgacggg gatcgaggga acgagaacca
     2821 ggctggagac ggcggctatc gcttcgagtg ccccgccctc ggcgctcacc agttgctcga
     2881 acacgaagat ccaggcgaac gtcagggctg ctgcgaaggc gaaccatttg atctgctggc
     2941 gctcgtcccc gctcgccccg cggaagcgta tgaagagtga gaccacggcg agtagcccgg
     3001 ccaacagcat gagtgggaac cccgcctcca cgatccacac gagccgtccc agtccctctt
     3061 ccccgtcacc tccgtcgctc aacaatgcct tcccattctc ggacaaggtc aggaccgcga
     3121 cgggaacact gatcaggccg atggagagtc cgccgagcca ggctacgggg cgccagcgcc
     3181 gggacggcgg acggccgtcg gggaacagga gcggaaggaa caccaggatc aggccgagcc
     3241 caggcgccca gacccactcg gccaaccacg acatccccgc cccgaagggg agtgatccgg
     3301 gcctggtgac gagcgcgtag gtggcgtact cgagcccgac ctcggagagc gcctggaaga
     3361 gggctgcggc gcagaagatc caacctatag tgttctccgg gcgatgggag acgataaggg
     3421 caccgaccac cgagaagctg atgacgagta ctacgacggc gcagtcaccc tccctcacga
     3481 acccgatcag ggtcctgccg ttcaggagcc agagcagggg ggcgcacagg gccaggccca
     3541 cgcacaaaag gcacaacgac cacgccagcc agaaaacgat cttcttatga tccatcaaca
     3601 tgaaaaccga accgccagcc tcctatcctc atggatgaac gatgacaccc ctcaaacctc
     3661 acaacatcct ccagatggac tagatctgcc aggttttcac cgtctccgcc gccgtcaggc
     3721 tctccgcaca ttaccagagg agcgtctcgc gatcgttaca caaagcgtta cgagagcctt
     3781 ctcttcaacg agttccccga taaaagcggg aagccgggca cctccgatgc taacctttgc
     3841 ctgaggagat cgaggagttg acgaggaatt gcgcgagata gcggccagca ctatcaccga
     3901 ggcggtgcgc gacctgtgca tcgaggcgaa cacgcgtctg ccgcaggagc acctcgccgc
     3961 cctgaggcgt gcgctcggag cagaggagtc ggaactcggg cgcgaggtga tcgaacgcct
     4021 cctggagaac gccgaagtcg cccgcgaacg gtgcgtcgcg ttctgccagg acacgggata
     4081 cgcggtcttc ttcgtcgaga tggggagcgg ggtcaggatc tcgtccggtg agatcggagc
     4141 ctccatcgac gagggcgtcc gacagggcta cgaggaaggc tacctgagaa aatccatagt
     4201 ccgaagccct attgacagga cgaacacggg cgacaacacc cccgccgtcg tctactacga
     4261 gccggtccca ggcgacggcc tgaaactgac gatgctcgtc aaaggcgcgg gctgcgacaa
     4321 catgagcgcc ctcaaaatgc tcacccccgc cgaaggtcta cccgcgatga agagcttcgt
     4381 cgtcgaaacc atcgagaggg caggcccgaa cgcgagcccg ccgctcaccg tcggagtcgg
     4441 catcggtggt cctttcgaga aggcagctca gctcgccaag aaggcgctca cccgcccgtc
     4501 cggtgagccg aaccccgacc cagaattggc cggcctcgaa cgcgagctgc tcgaagagat
     4561 caacgccaca ggcatcgggc ccgccggcta cggcggcacg cagacggcgc tcgcggtcca
     4621 cgtcgagagc ttcccgacgc acatcgccgc cttccccgtc gccgtcaacc tcgactgcca
     4681 ctcccaccgc accgcctcga tcgagctgtg acccccgagc ccatctacct ccatgcacca
     4741 ctctccatag aggacatcga acccttgagg acgggagacg tcgtgcgcct gaacggggtt
     4801 ctatacacgg cccgcgacgc ggcccacgcc aggatgaaag aagcactaga ggccggcgaa
     4861 ccgcttccct tcgatcccga gggccaggtc gtgtacttca ccggcccggc tcctgcgcgc
     4921 ccgggccatg cgctcggacc tgcggggccc acgacggcat cgaggatgga cccgtactca
     4981 ccattgctga tcgaacgcgg cctgagggga atggtcggga aaggttcacg ttcggaggct
     5041 gttctcgact cgatgcgaga gcacggttgc gtgtacttcg gagccgtcga gggtacggcg
     5101 gcgctgctcg cggactgtgt aaaggaggcc gaggtcatcg catacgagga cttgggcgcg
     5161 gaggcgatcc ggcgtctagt cgtggaagat ttcccagtag tagtcgtgaa cgatctctac
     5221 ggcggcgacc tttaccgcga gggtaaggag agatggcggc gctagcctga agcgtcgatc
     5281 ctcttttcta tcttctcgac gtactgttct ggtttggtct tgcgctccag gccgcctgcg
     5341 gacatgtagc ggaccttacc gaagacctcg cgctcggtcc agcccctatc gtgctggccg
     5401 aagatccagg cgacgttggt gaaggaattg gggtcgcgtc cgtcgaggaa gtacttgttg
     5461 ttgaggtaca gcgtggtcct gtaggcgtgc tcgggggtgt tcgaccactc caggatcttc
     5521 ttcccccagt acatgcgcat gtagttgtgc atgtagcccg tgtggaccat ctccttcatc
     5581 gccgtgttcc agtactcgtc gtgcgtctgg gcgctctcca agtgctttct cgtgtaagtg
     5641 tgctcgcgct cgtcgtcctt gtgttcttcg agggtcttcc tggcccagtc ggggaggcat
     5701 gagtacgaat cgtagtccgg ggtgtagtgg acgaagttca tggacagctc gcggcgtacg
     5761 atcagctcct cgacgaaaga ttcgatgttt gctttcttcg ttctggcttg tcgggcttcg
     5821 agggcgagcc agatcgggga tacgtgcccg aagtgtagat atttgctcat gtgcgatacg
     5881 tcgtcggtct gcggctggtt gcgatgcccg tcgtagttgg aaaagctgtc tttgagaaat
     5941 atgcggaaca ccttcttcgc ctccgaggtt ccgcctctgt acaggtggct gagtgggcca
     6001 acgctcctgt cgaggtccat gccgtcgagg actctctcga tgtcggagag gtcgaggcct
     6061 tcagccctca cgttcagcga ctgtttctcg atcttcatcg gcgtgagccc tacgaggaag
     6121 tcttcgagat attcttggat cttgggcctg agcgtcctcg ccgctgtctc ccgcttctct
     6181 gatgcgagct cgaccggcac gaccacattg gactcgacct gcgtcacgag gcatccggct
     6241 tctccggcga ctttctcgcg ccatctcctc tgagggcgca aatatccgcg gtcggtgacg
     6301 atgagggagg cgtctttgcc cgcgtcgagg gcgactgcat caggtgaacc cttgcggacg
     6361 accatcttga tgccgcgttc tttcagagct tcctggacgt cttcgaggcc ttccagcatg
     6421 aaggtgtagt gacggaggtt ggcttcgggg tagtcgtcgg ttaggccgaa gaccacgagc
     6481 aggcgctgac ccaggtcgtt cgccctttgg acagcgtatt cgagggcgtg gttctgctcg
     6541 gcacgctggg atgactgcat ccagtacagg acgtggtcgc cgtccctcac atccgcttcg
     6601 ttcaggttct gtacacgctc gttttgtatc ttccgtgtcg ccatctggtc tttgcctcca
     6661 gagttctcgc tcgtcttcgg gcaagcttag aggaacgcgc cgggaccgga tggagcagaa
     6721 aacacaaaac gccccctaca gcgctgtagg gggcgttctt gcaggctcgg gggttactcc
     6781 ggtttggctt ccgggtcgaa gcggagggcg gcggagttta tgcagtagcg ctggcccgtc
     6841 ggggcgggtc cgtcgtcgaa gacgtgtccc tggtgcgccc cgcaggtcgc gcagtgaacc
     6901 tcgatgcggc gcatgaagag actgcggtcc tcctctgtct cgacgttcga ctcgtcgagt
     6961 ggcgcccaga agctgggcca tcccgtaccc gactcgtact tggtgtcgga gctgaagagc
     7021 ggcgtcccgc aggctacgca gcggtagacc ccctcctcct tgttctcgta gtactcaccg
     7081 ctgaaaggcc gctccgtgcc cttcatccgc gtgatccggt actgctcgga ggtgagctgg
     7141 tcctgccact cttcttctgt cttctggatc ttcctcggtg tatcagtcat gcctgaattt
     7201 tatcacgccg tctcccgcct ccggagttca gagtgtgctc tctgagccga ggatggctgt
     7261 gacctgactc gagaggacgc ctccgttgcc gtggacgagg gcgatgtttg caacttcgac
     7321 ctgccgttcg ccgcattctc ctcggagctg ccgtacggcc tccacgatca ggagcagtcc
     7381 gtacatgcct ggatggttgt acgagagacc gcccccgctg gtgttgacgg cgagttctcc
     7441 tccgggggag atcctacctc cggagacgaa agtaccacct tccccctttt cgcagaaccc
     7501 gagatcttcc aggaacaaca aggtgtttat ggtgaaggcg tcatacagca tcgccacgtc
     7561 gatgtcctcg ggccccacgc cggccatctc gtatgccctc ggcccagatt ccgctgcagc
     7621 cgagaccgtg aggtcgggca tctggctcat gttgcggtgc cagtgggctt caccagcccc
     7681 gagaaggtag acaggacgct ctatgaaatc tcttgcccgt tcggcgctcg tgacgatggc
     7741 cgctccccca ccgtcggtca cgaggcagca atccctgaca gtcaggggcg aggagaccat
     7801 gcgcgaatcg agtacgtcct cgacggtgag gtcgtcgcgc atgaacgcct tcgggttgag
     7861 cttcgcccac tccctggccg cgacggctac ctcagcgagt tgctcccgcg tggttccgta
     7921 ctcgtgcatg tgccgggaag ctgcgagggc atacatgctc acggggtagc ggggtttgta
     7981 aggggcctcg tatgggtcgg cctccgagcc tgtgaccagg cgtcccccgc cggagcgctg
     8041 ggtcgagccg taggttatga gggcgacctc acatagcccg gcgtctatcg cggcggccgc
     8101 gtggaagagg tgggagacga acgatgagcc acccatgctc gtcgcgtccg agtagcgggg
     8161 cctgatgccg aggtactcgc ccactgagag ggtcggcatg tgatagtagg gcgaggccga
     8221 gaacagaccg tccacgtcgg acttttgcaa tccggcgtcg tcaagggcgc ggagtgtagc
     8281 ctgcccgatc aggtcaagag gcgtgaaccc cgcccccacc tcgccgaggt ccgattccgc
     8341 gaccccgacg acggcagcct tccccctgag gctcatctgt cctccggcac gaagacaggg
     8401 accggtacgt cgccttcacg gtcgatcctg agcgccaccc tcgttccgac ctccacttcc
     8461 tccgcgggca taccctttac gcggctcatc atcctgaagc cttcttccag atccacgagc
     8521 acgacgttgt aagggtctgc gtcccgacgg tacacggccg tcgtcgcgta aacgactccc
     8581 aggccagagc ttgtctccca aacgaggtct gcgcctccgc agacagagca caggacacgc
     8641 gggtagaaga ctgcagcccc acaaccggcg cagcgctgga agcctagctc tcctgactca
     8701 aggtagcccc tgtagatctc ggcgggtggc ccgccctctt cctggctcat ctcacctctc
     8761 tcatcatgga ccgagggtaa gcatccatga aagaaccagc aggcgcaagc cgggccagga
     8821 cttcgggtgc tgttgctagc ctcccggcat gagatcttta ggaccgccct tcatctatcc
     8881 cctgtctcaa gcaatgccac agggaagttg tgaagaaggc gtccgacatg gagcgactgc
     8941 tgaccgttat gctcctcggt cgtcaccttt tcgccggtga gcacgttgcg gtacgtgccc
     9001 tcagccagct cagccggaag aagtatggag gtatcttccc aggcttcggg agtaggaatc
     9061 agggaccctt ccacgtccat catcttcgca aggaggcgtg gcgccactgt tagcgctact
     9121 tcgcccccgt gcagcctggc gaaggcgacg atgtgttccc tccgtttacc cgacgtgtgg
     9181 agcgctacgt agccgccgtc cccgaagagt tccggcaatt cgctgcgcat cctcagcgct
     9241 ttccagatga gatagagctt cggacgaccg tcctgccacg cgccttcgtc gagaagcatg
     9301 cgcacgtcgg aggggtcgag ttctctcaga tgggcgagga gtctcttgcg tagcccgaag
     9361 tccaccggac gacggttatc cgggtcaacg aggctgaagt cccagatctc attgccctga
     9421 tacacgtcgg ggatgcctgg tgctgtaagt ttgatcaggc tctgggagag gctgttcaag
     9481 gcgccgatgc gggccacgcg acgttggaag ggcaggaatt ctcgcaagaa cgggttggac
     9541 tcagagagaa gggcgtcaac gaaaccggcg actgcatcct cgtacttctc gttcgcgtcg
     9601 gtccacgacg tgcgcacctg cgcctcccgc atcgccttct ccatgtactc ctttacgcgc
     9661 ttccgaaacg cttcctgctc ctttccgtcc agttctccca gcggccaggc accgagtagc
     9721 gtctggtaca ggaggtactc gtcgttgcgg ctcggggcga gttccccacc agcgtcgcgg
     9781 cggtgcgggg cattgagctc actccagcgc accacgctct cgcgccactc gccggggatc
     9841 tccgtgagta cgtcgatcct ggcgcgcacg tcctcgctgc gcttggtgtc gtgcgtggag
     9901 gtcgagagca tcgagtgcgg ccagcactca aggcgctcgg cgttcagacg gtgaaactcg
     9961 gggacggaga cgccgaagcg ctccggctcg ccgccgacct cgttcaggga gatcagtcgg
    10021 ttgtagacgt atagggccgc atcttccatg cccttcgcca tcaccggtcc cgtgtactgc
    10081 tgaaactttg acgtgaaggc gtccgcttcg a
//
LOCUS       DATAIR010000544         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_339281, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000544 DATAIR010000000
VERSION     DATAIR010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339281"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VHM69_12915"
     CDS             <1..793
                     /locus_tag="VHM69_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2741339.1"
                     /translation="EIGGVCLAAPGFILASENKIMSAANLEAIEGIPLKEELGSRTGL
                     RVTVENDANAAAWGEVRFGAGEDVDNLVFVTLGTGGGGGIISQGVLLRGARGMGGELG
                     HITIQPAGPRCGCGNRGCLEALASGTAIGRRAREVAGEQPDSALGRLAMERTPLGEDV
                     LTLARKGDGPALEVLREAGTWLGIGLATFVNVFDPEVIAIGGGVSEAGDLVLEPARRE
                     LRLRSCSPARDFVEIRGATLGAKSGILGAAALARGEDGEYVLGSS"
     gene            1002..2387
                     /locus_tag="VHM69_12920"
     CDS             1002..2387
                     /locus_tag="VHM69_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX2741340.1"
                     /translation="MNSSGGGMRGSVSRLNVYIAGTAAIIGLIYGYDLGSIASALLFL
                     VPDFDLSTFMTSAVTSAVVLGQLAGAFSAGRISNTIGRKRTMVAIALGYVLFAALQGL
                     APNEWFLLVIRFLLGFTIGLSIVVAPAYIAESAPRSVRGSMIVTFQIATVTGIAVAYF
                     VGAALAGTESWRLILSLSAIPALFVLFFVLRLPDTPRWYLMNGQREEALDLVAKVQGD
                     VNPEEEVRRIEEDLAYEEKGNFTELFQRRYRLATFFVITLGFLVQITGINAIVYFSPT
                     IIQEVGVTDPFRAIVVNGFIQLAGVVAVVGAFLVVDRWGRRPTLLTGVGVMVAANAIL
                     VVAYAMGPSIVLALVGILLFILGFNFGYGALVWVYASESFPARLRTQGGSAMLTSDLL
                     ANFIVGVVFLSALGALGGSLTFGIFFVLGIVSFVFIYALAPETKGRQLEAIRQYWYNG
                     AKWPDEEETRT"
     gene            2558..3511
                     /locus_tag="VHM69_12925"
     CDS             2558..3511
                     /locus_tag="VHM69_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX2741341.1"
                     /translation="MEASRDDVVEVVPDDFSYVFNPYREPVVRVEPGQRVVIHTEDAF
                     ESRINTVDDLPSRALATAKFLNPQTGPVYVEGAEPGDTLAVRLESIEPKRDYAVSCFV
                     PYFGGLTSTTLTRTLQEALPEKVYVWNLVEEGGERYVENDEVGVRLPWEPFVGTIAVA
                     PDLEAISALSPGPFGGNMDVPDVKPGNTVYLPVFNEGALFYTGDCHARQGQGELCGVA
                     LEITSQVTVSFEVIKGGGIEWPRIESGDRLMTVGSARPMEDAARIAYAELIGWLEDEH
                     GFSRPDAYQLLTQAGGLYVGNMVDTTYSLVASVEKQYLTRG"
     gene            complement(3579..3746)
                     /locus_tag="VHM69_12930"
     CDS             complement(3579..3746)
                     /locus_tag="VHM69_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741342.1"
                     /translation="MKNEMDAWYEEDEEVFVHARDPYHRVDVLENSRHVKDSVNGEVV
                     SETERPRTRWS"
     gene            4071..5729
                     /locus_tag="VHM69_12935"
     CDS             4071..5729
                     /locus_tag="VHM69_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2741343.1"
                     /translation="MTRIVLLQTDRARLVVTIVVAVLAALLAVSNYGNRSAHAASGCN
                     GPWGPGNWPPACWQPYADTSPFNTPLPPNPQLVTNSEQITDRIFTVRNSTNQGIAREA
                     HPANLAANWAGTAGEPTYYSRSNDPNFRLHCEGDPFGRPDGKCPIEGVVIDIPGGAGI
                     EYPPGSDHHLTVVDQAKGVEYDLWQVKAVDEAGPNPKKTVVDKLPTGLSNDPNVVHDL
                     YFSWGGRIDLYGLGFVDPNDKPHGFVGDATAAHFGSLAGRLRAEELAAGQIDHALFIG
                     VDCVGGAPVYPAQGQGRLCSTLGLSETDAPPMGTRLRLNMSEAQINGLNIDSWKKTIL
                     QALRKYGAFIGDTGTKGYFVIEAEAGVQYLTYGETDKWWAFGSALDPTQQRHVWNPWD
                     SNNDGVVDEYVGKLYNDDDDPRSGQPGFVNWESQVWENMEVVKPEVSKDTVAPAITRV
                     TPAPGSTGVSPTKNVSVLFSEAMQKSAVNGTTFSLMKKGTTTPVSASVSYNAATTTAT
                     LNPSRDLRRGATYIVTVKGGSSGVKDLSANPLAADKVWRFTVRS"
     gene            5881..>6307
                     /locus_tag="VHM69_12940"
     CDS             5881..>6307
                     /locus_tag="VHM69_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741344.1"
                     /translation="MRKIGVAAAVLLSLLIGIGVGTDDSPAAPTVKIASGDLAADIES
                     FVENIPRAYSEDYDVPTSAERSTMAAAHDAIEAGDLSQAASLADPLDYDVVRYEDTST
                     GRTLIMLSERSTEDAFGKKIWPHAWGMYIFSPHAGSDITV"
BASE COUNT         1234 a         1899 c         1982 g         1192 t
ORIGIN      
        1 cgagatagga ggcgtttgcc tggccgcccc cggcttcatc ctcgccagcg aaaacaagat
       61 catgagcgcg gccaacctcg aagctatcga gggcataccc ttgaaggagg aacttggcag
      121 tcgaacggga ctacgggtta ccgtggagaa cgacgccaac gccgcggcct ggggtgaggt
      181 ccgattcggt gccggagagg acgtcgataa cctggtcttc gtgacgcttg ggacgggggg
      241 ggggggtggg atcatctcgc aaggcgttct actcaggggc gcccgcggta tgggcgggga
      301 gttggggcac ataaccatcc agcccgccgg tcctcgctgc ggatgcggga acaggggatg
      361 tctcgaggcg ctggcctcgg gaacagctat agggcgtcgt gccagggagg tggcaggcga
      421 acagccggac tcggctctcg ggaggctagc catggagcgc acgccgctcg gtgaagacgt
      481 actgactctc gcccgcaaag gagatgggcc tgctctcgag gttctgcggg aggccggaac
      541 ctggctcggg ataggactcg ccaccttcgt aaacgtcttc gaccctgagg tgatcgccat
      601 aggcgggggt gtctcggagg cgggagacct ggtgcttgag cccgcccgca gggagctgcg
      661 tctgcgttcg tgctcgcccg cgagggactt cgtggagatc agaggcgcga cgctcggggc
      721 gaagtcaggc attcttggcg ccgcagcgct cgcccgcggt gaggacgggg agtacgtgct
      781 ggggtcctcc tgagcgtcct gcttgcgcgg tgtactcgaa cgccggtaga ataagcaatt
      841 gggggcatgc tattacaccg gcctgaacct tcggtgtggt tccggtcgcc gttggggagg
      901 gctttcgggg gaggattttt cgttcgagta agcttcgcac tttgggatcg agaaaaggtc
      961 acgcttcggg gaaggggtgt atcgtgtcag agggaggaac gatgaattca agcgggggag
     1021 gaatgcgggg gtccgtctcc aggctcaacg tgtatattgc cggcacggcg gcgatcatcg
     1081 gcctgatcta cggttacgac ctggggtcca tagcgagcgc gctcctcttc cttgtgcctg
     1141 acttcgatct ctccactttc atgacctcgg cggtgacctc ggcggtggtg ctcgggcagc
     1201 tggccggggc cttctcggcc ggtcgtatct ccaatacgat cgggcgcaaa cgcacaatgg
     1261 tcgcgatcgc gctcggctac gtgctcttcg ccgccctcca ggggctcgct cccaacgagt
     1321 ggttcttgct cgtgatccgg ttccttctcg ggttcaccat cggcctctcg atagtcgtcg
     1381 cgccggctta catcgctgag tccgcgccaa ggagcgtcag gggctccatg atcgtcacgt
     1441 tccagatcgc caccgtgaca ggcatcgccg tcgcctactt cgttggagca gccctcgccg
     1501 gcaccgagag ctggcgtctg atcctctcgc tctctgcgat cccagccctg ttcgtgctct
     1561 tcttcgtcct gcgtctgccc gacacccccc gctggtacct gatgaacggc caacgcgagg
     1621 aggctttgga cctggtagct aaagttcagg gagacgtgaa tcccgaagaa gaggtgcggc
     1681 gcatcgaaga agacctcgcc tatgaggaga agggcaactt cacggaactc ttccagaggc
     1741 gctatcgact ggccaccttc ttcgtcatca ccctcggctt cctggtccag atcacaggca
     1801 tcaacgccat cgtctatttc agcccgacga tcatccagga ggtcggcgtc accgacccgt
     1861 tcagggcgat cgtggtgaac ggtttcatcc agctcgccgg cgtggtagcg gtggttggcg
     1921 ccttcctcgt tgtggacagg tgggggcgca ggcccaccct gctgacgggt gtcggcgtca
     1981 tggtggcggc caacgcgatc ctcgtcgtcg cttatgcgat ggggccttcc atagtcctgg
     2041 ctctcgtcgg catcctcctc ttcatactgg gcttcaactt cggctacggc gcgctcgtgt
     2101 gggtctacgc ctccgagagc ttcccggcac gcctgcgtac ccagggcggg tcggcgatgt
     2161 tgacctcgga tctcctggct aactttatcg tcggcgtggt gttcctcagc gcgctcggcg
     2221 cacttggggg atcactgacc ttcggcatct tcttcgtact cgggatagtg tcgttcgtct
     2281 tcatatacgc ccttgccccc gaaaccaagg gacgccagct cgaggcgatc cggcaatact
     2341 ggtacaacgg cgccaagtgg ccggatgagg aggagacccg gacgtaacct gatctcgagc
     2401 cagcgcaaac cgggacctgg gtgaagggcc ctggttcacc cggagtgctg aaaatagatc
     2461 aaaccgaacg attccttgcg gatcgcttcg gtccctggtg gtacttgaac gggtgccaca
     2521 gaggggttga tagccatccc ggaaaggagg acagcaggtg gaggcatcga gggacgatgt
     2581 ggtggaggta gtgcccgacg acttcagcta cgtgttcaac ccttacaggg agcctgtggt
     2641 tcgcgtcgag cccggccaga gggtagtcat ccacaccgag gatgctttcg agagcaggat
     2701 caacaccgtg gacgacctgc cgagcagggc tctcgccacg gcgaagttcc tcaatcccca
     2761 gacggggccc gtctacgtcg agggagccga gccaggcgac accctcgccg tacgtctgga
     2821 gagcatcgag ccgaagcggg actacgcggt gagttgcttc gttccgtact tcggtgggtt
     2881 gacctctact actctcaccc gtactttgca ggaggcgctc ccggagaaag tctacgtatg
     2941 gaacctcgtc gaagagggcg gggagcgtta cgtggagaac gacgaggtcg gtgtcaggct
     3001 gccgtgggag ccgttcgtcg gcaccatcgc cgtggccccg gatctcgagg cgatctcagc
     3061 cctctcgccg ggacctttcg gtggcaacat ggacgtgccc gatgtgaagc cgggaaacac
     3121 cgtgtacctg ccggtcttca acgaaggggc tctcttctac acgggagact gccacgccag
     3181 gcaggggcag ggcgagctgt gcggcgtggc actcgagatc accagccagg tcaccgtctc
     3241 tttcgaggtg atcaaaggcg gaggcataga gtggccccgc atcgaatcag gagataggtt
     3301 gatgaccgtg ggaagcgcca ggccgatgga ggacgcggcc cgcatagcct acgcggagtt
     3361 gatcggctgg ctcgaagacg agcatggctt ctctcgcccg gacgcctacc agctcctgac
     3421 ccaggcagga ggcctctacg tgggaaacat ggtagacacc acctactccc tggtagcctc
     3481 cgtcgagaag cagtacctca cgcgtgggta ggccaacctc tccgagaaca caaagacagg
     3541 agagaagcta aagagcacat ccggtattgc gcctggacct acgaccagcg cgtcctgggg
     3601 cgctccgtct ccgacacgac ctctccattc acagagtcct tgacgtgccg cgagttctcc
     3661 agcacgtcca cccgatggta agggtcgcgg gcatggacga agacctcttc gtcctcctcg
     3721 taccacgcat ccatctcatt cttcatttac ttatgacctt cggctacggc tccgcgcttc
     3781 gctgaccgac ggtggcacag tgaagacaga accggagaag gctgcttacg cccctgatcg
     3841 tccctttcct actccgtaac aggttcgctg ccgaaggaac ttgatcgcac agttcgacgg
     3901 caacgccgac tcggcaataa gtccgccgat gtatagcgca aaccatgcgc tccggcagag
     3961 agcatccggg actcatcttc ccgaccttat cgggacagtg ttcccatgac ggtgggactt
     4021 acctccactt agtattccga cggcgaacga gcaaaggagg atgtacagat atgacccgca
     4081 tagtgttatt gcaaacggac cgagcaagac tcgtggtaac gatagtggtg gccgtgcttg
     4141 cggctttgct ggccgtctct aattacggga atcgctccgc gcacgcggcc tccggttgta
     4201 acgggccctg gggaccgggc aactggccgc cagcgtgctg gcagccctac gccgacacga
     4261 gccccttcaa tactcccctt cccccgaatc cccaattggt tacgaactcc gaacagataa
     4321 cggaccgcat cttcacagta cgcaacagca ccaatcaagg catcgcccgt gaagctcatc
     4381 cggcaaacct tgcggcaaac tgggcgggga ccgccggcga gccgacctac tactcgaggt
     4441 caaacgaccc gaatttcaga ctgcactgcg aaggagaccc tttcgggcga cctgacggga
     4501 agtgcccgat cgagggcgtg gtaatcgaca ttcctggtgg tgccgggata gaatatcctc
     4561 cgggttcgga ccaccacttg accgtcgtgg accaggcgaa gggcgtagaa tacgatctct
     4621 ggcaggtcaa ggccgtcgac gaggcgggac cgaacccgaa gaagacggtc gtggataaac
     4681 tcccgactgg cctttcgaac gacccaaacg ttgttcacga cctctacttt tcgtggggcg
     4741 ggcgtataga cctctacggt ctcggattcg tcgaccccaa cgacaaaccc cacggtttcg
     4801 tgggagacgc gaccgcggcc cacttcggca gcctcgcggg gcgcctcagg gccgaggagc
     4861 tcgctgcggg ccagatcgac cacgccctct ttatcggcgt agactgcgtc ggcggcgctc
     4921 cggtgtaccc ggcgcaggga cagggacgac tgtgctcgac gcttggtctc tcagagaccg
     4981 acgcgcctcc catgggcacg cgtctcaggt tgaacatgag cgaggcccag atcaacggcc
     5041 tcaacataga ctcttggaag aaaaccatcc tgcaggctct ccgcaaatac ggtgcgttca
     5101 tcggcgacac agggaccaaa ggctacttcg tcatcgaggc cgaggccgga gttcagtacc
     5161 tgacctacgg cgagaccgat aagtggtggg ccttcgggag tgccttggac cccacgcagc
     5221 aacgccatgt ctggaacccg tgggattcaa acaacgacgg tgtagtcgat gagtacgtcg
     5281 gcaagctgta caacgatgat gacgacccca ggtccgggca gcccggattc gtcaattggg
     5341 aatcgcaggt gtgggagaac atggaagtgg tcaagccgga agtatccaag gatacggtcg
     5401 cgcctgcgat caccagggtg acgccggctc ctggaagcac gggcgtctct ccgaccaaga
     5461 acgtctccgt gctcttctca gaggctatgc aaaagagtgc cgtaaacggg accaccttca
     5521 gcctgatgaa gaagggcacc actacgcctg tttccgcctc ggtaagctac aacgcggcca
     5581 ccacgacggc gacgctcaac ccgagccgcg acttgcgtcg tggggctacc tacatcgtca
     5641 ccgtcaaggg cgggagttca ggcgtgaaag acttgtccgc aaatcccctc gccgccgata
     5701 aggtctggag gttcacggtc aggagctagc ccgaagccag atcaacgggg taaccggggc
     5761 ccacaaactc cggttacccc tatcaggacc tcctaaggta cggatgcaga gggatgttat
     5821 gaggactgga tcgcttaaca ccaggttgac cgaacaatac tatcgcggga ggagttgatt
     5881 atgcgcaaga ttggcgtggc ggcagcggtg cttctctcgc tgctgatagg catcggcgtg
     5941 gggacagacg attcccctgc cgctcccacc gtcaagatcg cttcgggtga tctcgcggcc
     6001 gacatcgagt cgttcgtcga aaacatacca agagcatata gtgaagacta cgacgtgccg
     6061 acctccgctg agaggtcgac catggcggct gcgcacgacg ccatagaagc gggcgacctg
     6121 tcgcaagcgg catcactcgc ggatcccctc gattacgatg tcgtacgcta cgaggatacg
     6181 agcaccggtc ggacgctaat tatgctttcc gagcggtcaa cggaagacgc cttcgggaaa
     6241 aagatctggc ctcacgcctg gggtatgtat atattctctc ctcacgcggg atccgatatt
     6301 accgtag
//
LOCUS       DATAIR010000545         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_314012, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000545 DATAIR010000000
VERSION     DATAIR010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314012"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VHM69_12945"
     CDS             <1..713
                     /locus_tag="VHM69_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX2741345.1"
                     /translation="SGASPSKEHFCGATLIDSDSVLTAAHCADYIGAATIPGISVSYR
                     DVQIVVGLTELDSTQGQARKIQSLTDISIHPRYDRRRSTYDAAVIDLNEPVQDIAPIR
                     LATVVSKNRLERPGTSARIAGWGTTKIVGPFEQNRDNVSNRLRDATPPIVSDAECDRA
                     YNGDIQRTVQVCAGHRGEDSCQGDSGGPMWATTKTGRRQIGITSKGVGCGTERFPGVY
                     TEVNAPAIRTFITQVAGG"
     gene            complement(789..>2240)
                     /locus_tag="VHM69_12950"
     CDS             complement(789..>2240)
                     /locus_tag="VHM69_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741346.1"
                     /translation="KAREAIDSGAEEHCPTCHREFESGEQAEISDTLRRQAAAIRRRA
                     DRETEEGRKLAASADETAEKLKKVTSNLNRWRGLRETKVRAEDRAAGRLETLEKARSS
                     LEEIEAGIAGTDAPTEADLEAASTRCDRLRELRDVRPHIKSLAAEHSKLTQRVAELAD
                     ELEALSGVSYDPEAHLERREEKDRLERARGRIDELERRLATRPEVQRALEEARELGGK
                     AEAAAEELRREISTLAFDESAYEASVERVTAAEDRLDTLRNAREGLGGEWKEADHRIE
                     RARTELKRLDDDRKLANQRAAAASRMDEMDGLFTEFFRSLTARARPMLEVEASNLVRE
                     LTDGRYERMEFDDNYRVKLLDRFDDSYAIERFSGGESDVASLSARVALSRIVASRGGN
                     TLGFLVLDEVFGSLDAGRRNNVLLALERLKRSFGQIFLISHVAEVQEAALVDEVWMLE
                     EDEEGKSTVRLANSSPAPDDSVSRAITGTFSES"
BASE COUNT          365 a          749 c          700 g          426 t
ORIGIN      
        1 cgagcggcgc ttctcccagc aaggaacact tctgcggtgc caccctcatc gactccgaca
       61 gcgtgttgac ggctgcacac tgcgccgact acataggcgc cgctaccatt cccggtatct
      121 ccgtgtcata ccgcgacgtg cagatagtgg tcggcctgac cgagctcgac agcacgcagg
      181 gacaggcgcg taagatccag agcctcacgg acatctctat tcatccgcgc tacgaccgca
      241 ggaggagcac ttacgacgcc gctgtgatcg acttgaatga gcctgtacag gacatcgcac
      301 ccataagact cgccacggtg gtttccaaga acaggctcga acggccaggc acctcggcgc
      361 ggatcgccgg ttggggcaca acgaagatcg ttggcccatt tgagcaaaac cgggataacg
      421 tatcgaaccg tctgcgggac gcgacgccgc cgatcgtctc cgacgccgaa tgtgaccggg
      481 cctataacgg ggatatccaa agaactgtcc aggtctgtgc gggccatcgc ggagaggact
      541 cctgccaggg ggattcagga gggcctatgt gggccacgac gaagacggga cgacgacaga
      601 tcgggatcac cagcaaaggt gtgggttgcg gaacggaacg cttccccggc gtctacaccg
      661 aggtgaacgc acccgccata aggaccttca tcacccaggt tgccggcggc taagaggaag
      721 ctcgccgctg ccgtacccgg caatacctga cagaagacgc ggttcttcag gggcgtcgtc
      781 ctacgacgct acgattcgct gaaagtacct gtgatggctc tcgatacgga gtcatccggt
      841 gccggggacg agttcgccag tctgacggtg ctcttgcctt cctcgtcttc ttcgagcatc
      901 catacctcgt caacgagggc cgcttcctgc acctcggcga cgtgcgagat caggaagatc
      961 tggccgaacg agcgcttgag acgctcgagc gcgagtagca cgttgttgcg ccgccccgcg
     1021 tcgaggctgc cgaagacctc gtcgaggacg aggaagccga gagtgttgcc gccgcgggag
     1081 gccacaatcc tggagagcgc caccctcgcc gagaggctgg cgacgtccga ctcgccgcct
     1141 gagaagcgct cgatggcgta tgagtcgtcg aagcggtcga ggagcttcac gcggtagttg
     1201 tcgtcgaact ccatccgctc gtagcggccg tccgttagct cgcgcacgag gttggaggcc
     1261 tcgacctcga gcatgggacg cgccctcgcg gtcaggcttc tgaagaactc ggtgaataga
     1321 ccgtccatct cgtccatacg cgaggccgcg gcggccctct ggttggcgag cttcctgtcg
     1381 tcgtcgaggc gcttgagctc ggtcctggca cgctcgatgc ggtggtctgc ctccttccac
     1441 tcgcctccga ggccctccct cgcgttccgc agcgtatcga gccggtcctc cgccgccgtc
     1501 acgcgctcca cggaagcctc gtaggccgat tcgtcgaagg caagcgtgga gatctcccgc
     1561 cgcaattctt cggccgccgc ttccgccttt ccccccaact cacgggcctc ctcgagcgct
     1621 ctctggactt cgggacgagt cgcgaggcgg cgctcaagct catcgatcct tccgcgggcg
     1681 cgctccagcc tgtctttctc ctcccgcctt tccaggtgcg cttctgggtc gtacgagacg
     1741 cccgagagcg cttcgagctc gtcagcgagc tcggcgacgc gctgcgtgag cttcgagtgt
     1801 tcggccgcca ggctctttat gtgcggacgg acgtcgcgca actctctcaa gcggtcgcag
     1861 cgggtgctgg ccgcttccag gtcggcttct gttggagcgt ccgtgcctgc aataccggct
     1921 tcgatctcct ccaggctgga gcgggctttc tcaagcgttt cgagacggcc tgcggcgcgg
     1981 tcctcggcac gaaccttcgt ctcgcgcagc ccgcgccagc ggttcaggtt cgatgtgacc
     2041 tttttcagct tctcggcggt ctcgtccgca gaggcggcga gctttctgcc ctcctccgtc
     2101 tcgcggtcgg cgcggcgacg gatggcggcc gcctgcctcc tcagggtatc ggagatctcc
     2161 gcctgctccc cgctctcgaa ctccctgtgg caggtggggc agtgctcctc ggcgccgctg
     2221 tcgatggctt ctctggcctt
//
LOCUS       DATAIR010000546         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_247612, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000546 DATAIR010000000
VERSION     DATAIR010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247612"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHM69_12955"
     CDS             <1..280
                     /locus_tag="VHM69_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741347.1"
                     /translation="GAGVAYATGSGESGEQQATGPGIEKAKSVALDHTNGGRVTGTEV
                     GDEEGYYEVEVIREDGSQVDVHLDRDFNVLGTPADNEGPDDKDAPNDD"
     gene            404..1081
                     /locus_tag="VHM69_12960"
     CDS             404..1081
                     /locus_tag="VHM69_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741348.1"
                     /translation="MRAGTRVLLVEDDPSIVGFVEPELQHVGFHVRCARDGLTGLQQA
                     QLFGPDIVVLDIMLPKLDGVGVLKRLRHGGCGVPIVMLTARDATIDKVHSLDLGADDY
                     LTKPFDIDELLARMRALLRRVEGDEILRVADLEVNTTTREVRRGEREIFLTAREYDLL
                     EFMALNPRRVLSRDLLLMRVWDREFAVTANSVEVYIAYLRKKVDAPGERKLIHTIRGA
                     GYALKGT"
     gene            1087..2487
                     /locus_tag="VHM69_12965"
     CDS             1087..2487
                     /locus_tag="VHM69_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2741349.1"
                     /translation="MPIRWRLTLYIALVIGAILIILGFALYFLSRSVLLSGVEDTARS
                     RATAAAKLIDSGESLSDAYVEQLTLDGVVVIVRDGDGGVLRRVNIPAKGDAESAVWRE
                     AMGSGEPASGTAVLSGDDPYYVYAMPVDPPEGPARVVEAAKPYEPAQDALEVFETVLV
                     SGIGAALLLSIGGAYLLAGAALGPVEAVTRAAGSMGEGELSKRLPVANPNDEVGRLAA
                     TINGLLSRLEAAFARREEALERQRRFAADASHELRTPLTAISGHARMLEEWALEEDPK
                     RAKRSVRAIRVAAGRMRNLVESLLILTRGDESTVLDVGSNDLASVAEEATQVARTAAD
                     EKVSIEYVSPKRPVEATFDRDRVFQVASILLDNAVKYTHQGGSVLVRVEEEDGGAVLE
                     VSDTGVGIPEDELPLVFERFHRTDDSRTEGGVGLGLSIARQIAEAHGGEIRAKSAQGK
                     GSTFTLLLPNRPQPDP"
BASE COUNT          507 a          808 c          845 g          436 t
ORIGIN      
        1 cggggcaggt gtcgcttatg ctaccggctc cggcgaatct ggagagcagc aggccaccgg
       61 acccggcatc gagaaggcca agagcgtcgc gctcgaccac accaacggag gccgtgtgac
      121 cggcaccgag gtcggcgacg aagagggcta ctacgaggtc gaggtcatcc gtgaagacgg
      181 aagccaggtc gacgttcacc tcgaccgcga cttcaatgtc ctcggcactc ctgcagataa
      241 cgaaggcccc gacgacaagg acgcgcccaa cgacgactaa catccgagcc gtgaaggcct
      301 ggatgcaata ctgcatttgg tgagggccgg gatcagttgt cccggccctt actcttgccg
      361 ggagatcggg gattagaata tcgatgcgag aggtggtgaa aaattgagag ctggaacgcg
      421 cgtgttgctc gttgaggatg acccctcgat cgtcggcttc gtcgagcccg agctccagca
      481 tgtcggtttc cacgtgcgct gcgctcgcga cggtctcacc ggactccagc aggctcaact
      541 attcgggcca gatatcgtgg tccttgacat catgctgcca aaactcgatg gggtgggggt
      601 cttgaagagg ctcaggcatg gagggtgcgg ggtgcctatc gtcatgctca cggcccgcga
      661 cgccaccatc gacaaggtcc acagcctcga cctcggcgcc gacgactacc tcaccaagcc
      721 gttcgatatc gacgagctcc tcgcgcgtat gcgtgctctc ctaaggcgcg tggagggcga
      781 tgagatactc agggtcgccg accttgaagt gaacaccaca acacgcgagg ttcggcgcgg
      841 ggagcgggag atattcctca ccgcccgaga gtacgatctg ctggagttca tggcactcaa
      901 tccccgccgg gtcctctcca gggacctact cctgatgagg gtatgggaca gggagttcgc
      961 cgtcacggcg aactccgtcg aggtctacat cgcctacctc aggaagaagg tcgatgcacc
     1021 gggggagcgc aagctcatcc acaccatcag gggcgcggga tacgccttga agggtaccta
     1081 gcacgcatgc cgatccggtg gcggctgacg ctatacatcg cccttgtgat cggcgcgatc
     1141 ctgatcatcc ttggtttcgc tctgtacttt ctgagcagga gcgtgcttct ctctggcgtg
     1201 gaggataccg caaggagccg ggcaaccgcc gccgccaaac tcatcgattc cggagagagc
     1261 ttgagcgacg cctatgtcga gcagctcacc ctcgatgggg tagtcgtgat cgtccgtgat
     1321 ggagacggcg gcgtcctgcg gagggtgaac attcccgcga aaggtgatgc cgaaagcgcc
     1381 gtctggcgcg aggcgatggg ctcgggagag ccggcctcgg ggacggctgt gctctctggg
     1441 gatgatccct actacgtcta cgccatgccc gtggacccgc cggagggacc ggcgcgggtg
     1501 gtcgaagcgg ccaagccata tgagccagca caggatgccc tcgaagtctt cgaaaccgtt
     1561 ctcgtatccg gcatcggcgc tgccctcctt ctgtccatcg gtggagctta cttgctggct
     1621 ggggccgccc tcggacccgt cgaggcggta acgagagccg ccgggtcgat gggagaaggc
     1681 gagctttcga agcggttgcc agtggcgaac cccaacgacg aagtcggtcg tctggccgcc
     1741 acgatcaacg gcctcctctc tcgtctcgaa gccgccttcg cgcgccgcga ggaggcactg
     1801 gaacgccagc ggcgcttcgc cgccgacgcc agccacgagc tgcgcacgcc gcttaccgcg
     1861 ataagcggcc acgccaggat gcttgaggag tgggcgctcg aagaggaccc caagagggct
     1921 aaaaggagcg tcagggccat ccgcgtggcg gctggaagga tgcgcaacct ggtcgaatcg
     1981 ctcctgatcc tgacccgcgg cgacgagagc acggtcctcg acgtcggaag caacgacctc
     2041 gcctccgtcg ccgaggaggc aactcaggta gccaggaccg ccgccgacga gaaggtctca
     2101 atcgagtacg tcagcccgaa gcgtccggtc gaggcaacct tcgacaggga ccgtgtcttt
     2161 caggtcgcct ccattctcct agacaacgcc gtgaagtaca cgcaccaagg tgggagcgtc
     2221 cttgtgaggg tggaggagga agacggtggg gccgtgctgg aggtctcgga cacgggtgtc
     2281 ggcatcccag aggatgagct gcccctggtc ttcgagcgct tccacaggac ggacgactcc
     2341 cgcacggaag ggggagtggg tcttgggctc tcgatagcgc gtcagatcgc cgaggcccac
     2401 ggcggcgaga taagggcgaa gagcgcacaa ggtaagggtt cgactttcac acttctactc
     2461 cctaatcggc cgcagccaga cccctaaacg ctcagtcctc acgctcgccg tgccacccga
     2521 gcaaacacct cgggccactg tgtgcctggc cgacgccgaa gcagccccgg ctacatcctc
     2581 agcaacctct caggat
//
LOCUS       DATAIR010000547         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_452175, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000547 DATAIR010000000
VERSION     DATAIR010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452175"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VHM69_12970"
     CDS             complement(<1..315)
                     /locus_tag="VHM69_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX2741350.1"
                     /translation="MLNSEELAVLEQEIVSCRRCPRLVAWREEVAREKRASYSDETYW
                     GRPVPGFGDPDASVVVFGLAPAAHGANRTGRVFTGDRSGDFLFAALHRTGFANKPISR
                     HKD"
     gene            complement(333..>1545)
                     /locus_tag="VHM69_12975"
     CDS             complement(333..>1545)
                     /locus_tag="VHM69_12975"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX2741351.1"
                     /translation="VLDLNLMRELSVKTDTKIVLLVIDGLGGLPMDPSGETELEAANT
                     PNLDALAARSDLGLSRPVAAGVSPGSGPGHLGLFGYDPVSFQVGRGVLSALGVGFDLQ
                     KSDLAARVNFATKDESGKISDRRAGRIPSEKGEELVALLNEKVKIDGVEVSVMHEKEY
                     RAVAVFRGDGLSDALADSDPQRTGLEPLPVKPTDESSEANNSAQIANAFVEQANEILA
                     DQHPANTVLVRGFGMHPALPSFEEAYKLDAAAIASYPMYKGLARLAGMQLLKEGEGIA
                     GEFETLKASWDDHDFFFVHIKPTDAAGEDGDFDRKASVIEEVDAQIPGLLDLGPDALA
                     ITGDHATPAKMKSHSWHGVPFLLNSPYTLPTAEAFGERPCAGGSLGVLQAEEIMGYLM
                     GNALKLNRFGA"
BASE COUNT          240 a          482 c          539 g          284 t
ORIGIN      
        1 gtctttgtgt cgcgagatgg gcttgttggc gaaacccgtg cggtggagcg ccgcgaagag
       61 gaagtcgccg gagcggtcgc ccgtgaagac gcgccctgtc ctgttcgcac cgtgggcggc
      121 aggggcgagg ccgaagacca cgacgcttgc gtcagggtcg ccgaagccgg ggacgggacg
      181 tccccagtaa gtctcgtcag agtaggaagc cctcttctcc ctggcgacct cctctcgcca
      241 ggcgacgagg cgagggcagc gacggcagga gacgatttct tgttcgagaa cagcgagttc
      301 ctcactgttc agcacgctgc gccagcgtgc tctcacgcgc cgaagcggtt tagtttcagg
      361 gcgttgccca tgaggtagcc catgatctcc tctgcctgca acacgccgag cgagcctcca
      421 gcgcacgggc gctctccgaa ggcctccgcg gtcggcagcg tgtacggtga gttgagcaag
      481 aacggcaccc cgtgccacga gtggctcttc atcttcgccg gtgtcgcgtg gtcgccggtt
      541 atggcgaggg catcgggacc gaggtcgaga aggcccggta tctgggcgtc tacctcctcg
      601 atgacggagg ctttcctgtc gaagtccccg tcctccccgg cggcgtcggt cggcttgatg
      661 tggacgaaga agaagtcgtg gtcgtcccag ctcgccttaa gcgtctcgaa ctctcctgcg
      721 atgccctcgc cttccttgag gagctgcatc cccgcgagac gggcgaggcc cttgtacatc
      781 gggtagctcg cgatggcggc ggcgtccagc ttgtaggcct cctcgaaaga ggggagggcg
      841 ggatgcatcc cgaagccgcg cacgaggacg gtgttagcgg ggtgctggtc tgcgaggatc
      901 tcgttggcct gctcgacgaa ggcgttggcg atctgggcgc tgttgttggc ttccgaggac
      961 tcgtccgtcg gcttgacggg gagcggttcg aggccggtgc gctgggggtc cgagtctgcc
     1021 agggcgtcag agagaccatc acccctgaag acggccacgg ccctgtactc cttctcgtgc
     1081 atgacggaga cctcgacgcc gtcgatcttc accttctcgt tcagaagcgc gacgagctcc
     1141 tcgcccttct ctgaggggat acggcccgcg cgtcggtccg agatcttgcc cgattcgtct
     1201 ttggtggcga agtttacgcg ggccgcgagg tcgctcttct gcaggtcgaa gccgacgccg
     1261 agggcagaga gcacgccgcg tcctacctgg aagctcacgg ggtcgtaccc gaagaggccg
     1321 aggtgaccgg ggccgctacc agggctcacc cctgccgcga cgggtcgcga gagcccgagg
     1381 tcagatcgcg cggcgagggc gtcgaggttc ggggtgttgg cggcctccag ctccgtctcg
     1441 cccgaagggt ccatcggcag cccgcccagt ccgtcgatga cgagcaggac gatcttcgtg
     1501 tccgtcttca cggatagctc gcgcatgagg ttgagatcca agaca
//
LOCUS       DATAIR010000548         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_452282, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000548 DATAIR010000000
VERSION     DATAIR010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452282"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHM69_12980"
     CDS             complement(<1..204)
                     /locus_tag="VHM69_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741352.1"
                     /translation="MITSENLQQRDVTFKPGDTVRVHYRLVGGCGTSESVSRARSWDG
                     LSSIYDGPLIAYPITRFGSVEYGY"
     gene            complement(201..929)
                     /gene="trmD"
                     /locus_tag="VHM69_12985"
     CDS             complement(201..929)
                     /gene="trmD"
                     /locus_tag="VHM69_12985"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2741353.1"
                     /translation="MRNIDVFCVFPEAVDAAVRVGVVGRAIENGIVGYRSFDLRDFSP
                     DGRIDDMPYGGGAGMVVRVDVVARALEEVYGVPAKEVKAKRRVVLTEPAGRRLTQGYV
                     DEVAAGPDLTVICGRYGGMDERVWTELATEAVSLGAFVLSGGEIVAAALADAAIRRQQ
                     GVLGNAESLVGESFSAEDVVGPPTYTRPAVWDGEEVPGVLLSGDHAQIRAWREREARE
                     RTLARTTGKRSPGHPEIHPREEKA"
     gene            complement(934..1425)
                     /gene="rimM"
                     /locus_tag="VHM69_12990"
     CDS             complement(934..1425)
                     /gene="rimM"
                     /locus_tag="VHM69_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2741354.1"
                     /translation="MEGLSDPVPIGVVVAPHGVSGTLRVRALGSGRHLRKESEPVVAG
                     VRRRISTSRQTPKGFLLDFEGIESRAAADSLRGEELFLDRRELDAPDAGEVYVADLVG
                     LTVVNDAGDVVGTVKDTFETAAHEVLIVSKEDDELYVPFTLEHVPVLDLETGRVVIRP
                     PES"
     gene            complement(1431..1649)
                     /locus_tag="VHM69_12995"
     CDS             complement(1431..1649)
                     /locus_tag="VHM69_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX2741355.1"
                     /translation="MLVLARSLVDEPEQVEVSGTESDSRVDLELRVAPGDMGRVIGRQ
                     GRTIRAIRTVVKAASVKLGKRVTVEVPD"
     gene            complement(1677..1919)
                     /gene="rpsP"
                     /locus_tag="VHM69_13000"
     CDS             complement(1677..1919)
                     /gene="rpsP"
                     /locus_tag="VHM69_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2741356.1"
                     /translation="MAVKIRLARHGSKGNPFYRIVVADSRSPRDGRFIDRIGTYDPQK
                     EPSDIKVDEEKAREWLGKGAQPTDQVRNLLKISGVL"
     gene            complement(1939..3270)
                     /gene="ffh"
                     /locus_tag="VHM69_13005"
     CDS             complement(1939..3270)
                     /gene="ffh"
                     /locus_tag="VHM69_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX2741357.1"
                     /translation="MFDTLGERLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YGVVKEFVSNVRERATGADVSKALSPGQQVVKIVNEELANLMGGTAHKLSFASKPPTV
                     VMLAGLNGHGKTTTAGKLALFVRKLGKNPFLIAGDTYRPAAIDQLQQIGRELSVPVYT
                     EGTGPKPEEITERGIREARDAGYDFVIVDTAGRQVVDMEMMEEIRRVRKVSRPHSVLL
                     VLDVVTGQNAVEVAQAFQEYADFDGMVLTKLDGDARGGAALSVVSVTGRPVKFASEGE
                     KMGEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDRSEAEAQGKKLMSGKFTFEDFLQ
                     QMQMIKKMGPLSGLLGMVPGLGKQLKDVEIDDRQLGRVEAMITSMTVQERRNPKLLQN
                     PSRARRIARGSGATQQDVQKLVKQFKEMQKMMAQMGKGGGMPRIPGLPGRR"
     gene            complement(3271..>3591)
                     /locus_tag="VHM69_13010"
     CDS             complement(3271..>3591)
                     /locus_tag="VHM69_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX2741358.1"
                     /translation="VNLMAELDKVQRVIQRQLPGAPHEVLMTVDATTGQNGLRQAKMF
                     KEVAGLTGIILTKLDGTAKGGIALAIANEVGVPIKLISVGEKVEDLHPFDAREFAEAL
                     FGDL"
BASE COUNT          606 a         1269 c          994 g          722 t
ORIGIN      
        1 atacccgtac tcaacgcttc cgaaacgggt tatgggataa gcgatcaacg gcccgtcgta
       61 tatagaggaa aggccgtccc acgagcgggc acgggagacg ctttcagacg ttccacatcc
      121 acccaccagg cggtagtgga cccgcacggt gtcgcccggc ttgaacgtga cgtcccgctg
      181 ctgcaaattc tcgctcgtta tcatgctttc tcctctcgtg ggtggatctc cggatggccg
      241 ggcgacctct ttccggtcgt cctggccagc gtacgctctc gcgcctcgcg ctcgcgccag
      301 gcgcgtatct gcgcatgatc gcccgataat agcacgcctg gcacctcctc tccatcccat
      361 acggctggcc tcgtatacgt tggagggcca accacatcct ccgccgagaa cgactcgccc
      421 acgaggctct ccgcgttccc gaggactccc tgttggcgcc gtatcgcggc gtccgcgagt
      481 gccgcggcga cgatttcacc tcccgaaagc acgaaggcgc ccagcgagac tgcctccgtc
      541 gcgagctccg tccacacccg ctcgtccatc ccgccgtagc gtccacagat gacggtgaga
      601 tccggtcccg ctgccacctc gtccacgtaa ccctgcgtga gcctccggcc cgctggctcc
      661 gtgagaacga cgcgacgttt ggccttcacc tctttcgccg gaacgccgta gacttcctcg
      721 agcgcccgcg cgacgacgtc gacccggacg accattccgg caccaccacc gtaaggcatg
      781 tcgtcgatgc ggccgtccgg ggagaagtca cggaggtcga agctccggta gccgacgata
      841 ccattttcta tcgccctgcc gacgaccccg acacgcacag ccgcgtcgac ggcttcgggg
      901 aagacgcaga aaacgtctat gttcctcatg gcattagctc tctgggggcc ggatcacgac
      961 gcgccccgtc tcaagatcca gcacggggac gtgctcgaga gtaaatggga cgtatagttc
     1021 atcgtcctct ttgctgacaa taagcacttc gtgtgccgcg gtctcgaaag tatccttcac
     1081 cgtacctacg acgtcaccgg catcgttcac gaccgtcagg ccgaccagat cagcgacgta
     1141 gacctcgcct gcatccggcg cgtcgagctc tctccggtcc aggaacaatt cttcgcctct
     1201 gagggagtcc gcagcggccc tgctttcgat gccttcgaag tccagcagga accccttagg
     1261 cgtctgcctg gaggtagaga tccgacgcct aaccccggcc acgaccggtt cagactcttt
     1321 cctgaggtgt cggcctgagc cgagggcgcg gaccctcagg gtgccgctca ccccgtgcgg
     1381 tgcgaccacg accccgatcg gcaccgggtc ggagagtcct tccacggtct ctagtccggg
     1441 acctccacgg tgacccgctt gccgagcttt acggaagcgg ccttcacgac cgtgcggatc
     1501 gcccggatcg tacgtccctg gcgaccgatc acacgcccca tgtcgccagg cgccaccctg
     1561 agctcgaggt cgacgcgcga gtcggactcc gtccccgaga cctcgacctg ctccggctcg
     1621 tccaccagcg agcgggccaa taccagcaac aagttctcca gttgcgccac ggccggctag
     1681 aggaccccgg agatcttcaa caggttacgc acctggtccg tcggctgcgc ccccttgccg
     1741 agccactccc tcgccttctc ctcgtccacc ttgatatcag aaggctcctt ctgcggatcg
     1801 taggtcccga tcctgtctat gaatcgcccg tcgcgcgggc tccgtgaatc agcgacgact
     1861 atcctgtaga agggattgcc cttcgaaccg tgcctagcga gcctgatctt taccgccata
     1921 ctatctcctc tcgaactttt acctgcgccc cggcagaccg gggatacgcg gcataccgcc
     1981 gcccttgccc atctgggcca tcatcttctg catctccttg aactgcttga cgagcttctg
     2041 gacgtcctgc tgggtcgccc cagagccccg cgcaatccta cgcgcccggc tcgggttctg
     2101 caacaacttg gggttgcgcc gctcctggac ggtcatcgag gtgatcatcg cctcgaccct
     2161 gccgagctgc ctgtcatcta tctccacgtc ctttagctgc ttgccaaggc cggggaccat
     2221 gcccaatagt cccgacagcg gtcccatctt cttgatcatc tgcatctgct gcagaaagtc
     2281 ctcgaaggtg aatttgccgc tcatgagctt cttgccctgg gcctcagcct ccgaacggtc
     2341 catctgctgc tcggccttct cgattagcgt tagcacgtca cccatcccga ggatgcgacc
     2401 tgccatccga tccgggtaga agtagtcgaa ctcgcccatc ttctcgccct ctgaggcgaa
     2461 ctttacggga cgccccgtca cggagactac ggagagcgcg gccccaccgc gggcgtcgcc
     2521 gtctagcttg gtgagcacca tcccgtcgaa gtcggcgtac tcctggaagg cctgggcgac
     2581 ctcgacggcg ttctggccgg tgacaacgtc gagtaccagg aggaccgagt gcggtctcga
     2641 aaccttcctg acgcgccgga tctcctccat catctccatg tcgacgacct gccggccagc
     2701 cgtatcgacg atgacgaagt cgtaccctgc gtccctggcc tcccggatgc ccctctcggt
     2761 gatctcctcc ggcttcggcc ccgtgccctc ggtatagaca ggcacggata gctcgcgccc
     2821 gatctgctgc agctggtcta tggccgcagg acgataggtg tccccggcga taagaaaagg
     2881 gtttttgccg agcttcctta cgaacaacgc cagcttaccg gccgtcgtcg tcttgccgtg
     2941 gccgttgagg ccggcgagca taacgacagt tggcggcttc gaggcgaagg atagcttgtg
     3001 ggccgtgccg cccataaggt tcgcaagctc ctcgtttact atcttcacga cctgctgccc
     3061 ggggctgagg gccttcgaca cgtcggctcc cgtcgccctc tcgcgcacgt tggagacgaa
     3121 ctccttcacg acgccatagt tcacgtccgc ctccagcaag gccagacgga tctctcgcgt
     3181 aaccttcttt acctggtcct cggtgaggtt gccgctgcct cggaccccgt cgagggcctt
     3241 gttgagcctt tcacctaaag tatcgaacat ctatagatct ccaaacaatg cttccgcgaa
     3301 ctccctggcg tcgaacgggt gaaggtcctc gaccttctcc ccaaccgaga tgagcttgat
     3361 cggcaccccg acctcgttgg ctatggcgag cgcgatgccg cccttggccg tcccgtcgag
     3421 cttggtgagg attatgccgg tgagccccgc gacctctttg aacatcttgg cctgccgcaa
     3481 cccgttctga ccggtggtcg catccaccgt catgagcacc tcgtgcggcg cgcctggaag
     3541 ctggcgctgt attactctct gcactttgtc cagttccgcc atgaggttga c
//
LOCUS       DATAIR010000549         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_452346, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000549 DATAIR010000000
VERSION     DATAIR010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452346"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VHM69_13015"
     CDS             <1..1169
                     /locus_tag="VHM69_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741359.1"
                     /translation="EEEKLLDRFRDPLDPLQFLIVTSKLLTGFDAPILQAMYLDKPMR
                     DHNLLQAICRTNRVYPGKTHGLIVDYLGVFDDVANALDFDERAVQQVITNLDELKERL
                     PTQIEACLHFFPDVDRTIGGYEGLMAAQECLPNNERRDRFAEQYSVLSRLWEALSPDP
                     VLEPHEADYGWLTQVYESVKPPSGNGKLLWHALGAKTLDLVHRNTHIEDVRDDLETLV
                     LDAEVLEGLLGTDDPEGKSREIEVKLISRLNRHGDNPTFVALGERLERLKERHEQGQL
                     HSIKFLKELLTLAREVVEAEQQIDPVDEQARAKAALTELFAETRNGETPVIVERIVAD
                     IDNIVRIVRFPGWQGTKAGEREVQKALRKVVHVDYKIKDQDLFDKAFGYIRQYY"
     gene            1657..2217
                     /locus_tag="VHM69_13020"
     CDS             1657..2217
                     /locus_tag="VHM69_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741360.1"
                     /translation="MLYAPVFVVHDCGFQARCALEIDDSGRVRIENIVELIQGCRYGI
                     HDISRTELDTENKLPRFNMPLELGLFLGATRFGSPKQKRKRSLILDREPYRYQKYISD
                     ISGQDIKSHDGDPKTAIRQVRDWLRNSTTMTDTIIPGPGRIIERYELFREELPELCRR
                     SHLDPDDLIFNDYTTCVSEWLKENDW"
     gene            complement(2282..>3246)
                     /locus_tag="VHM69_13025"
     CDS             complement(2282..>3246)
                     /locus_tag="VHM69_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX2741361.1"
                     /translation="LSGASSDAAGYTMSETYDLGHLFEIMEEVARVQAGRNDGSRVSL
                     SIHCEQPELIRIFNERVQREGVLEGLEAYSASRPTLTEHLAVAEVGVLAGHTRCPVNL
                     LHLSSEEAFEAALDLKRKHPSLDARLETTLHHLALTYETYNDQRGKVNPPIRAQSDVD
                     ALWRGVLRGEIDWVCSDHACCSEENKEGDLWQALPGFGGTALIYPFMLTEGLRRGASL
                     ERIVDLVSTNPARAYGLTPRKGTITIGADADLAIVNLEDTHPVTTERLLSAQEYTPFE
                     GMELAGWPVRTLLRGKTVFADGEATGQPTGEYLKRPLTGAQTPG"
BASE COUNT          670 a          988 c          996 g          592 t
ORIGIN      
        1 acgaagaaga gaagctcctc gaccgcttcc gcgacccgct cgacccacta cagttcctca
       61 tcgtcacctc caagctcttg accggcttcg acgcgcccat attgcaggcc atgtacctgg
      121 acaagccgat gcgggatcat aacctgttgc aggccatctg ccgcaccaac cgcgtatatc
      181 cgggaaagac gcacggcctg atcgtggact acctcggcgt cttcgacgac gtagcaaacg
      241 ccctggactt cgacgagcgg gccgtccagc aggtaatcac caacctcgac gagctgaagg
      301 aacgcttgcc aacacagatc gaagcgtgcc tgcacttctt ccctgacgtg gaccgcacca
      361 ttggcggcta cgagggcctg atggccgccc aggaatgcct gccgaacaac gaaaggcggg
      421 accgcttcgc cgagcagtat tctgtgctct cgcgcctgtg ggaagcactc tcgcctgacc
      481 cggtgctgga gccccacgag gcagactacg ggtggctcac ccaggtctac gaatccgtca
      541 agccgcctag cggcaacgga aaattgctgt ggcatgcgct cggggcgaag accctggatc
      601 tcgtccacag gaacacgcac atcgaggacg tgcgcgacga cctggagacc cttgtgctag
      661 atgcggaggt gctggaaggt ctgctcggca ccgacgaccc ggagggcaag tccagggaga
      721 tagaggtcaa gcttatctcc cgcctgaacc ggcatgggga caaccccacg ttcgtggcac
      781 ttggtgagcg gctggagagg ctcaaggagc ggcacgaaca gggccagttg cacagcatca
      841 agtttctcaa agagctcctg acgcttgcca gagaagtagt cgaggcggag cagcagatag
      901 accccgtaga cgagcaggcg agggccaagg ccgcgctgac agagctgttc gccgagacca
      961 ggaatggtga gacgcctgtg atcgtggagc gaatagtcgc tgacatagac aacatcgtgc
     1021 ggatcgtgcg gttccctggt tggcagggca ccaaggcggg ggagcgggag gtgcagaagg
     1081 cgctgcgcaa ggtggtccac gtggactaca agatcaaaga ccaggacctc ttcgacaaag
     1141 ccttcgggta catccgccag tactactgaa catctcgaag gcggtagacc gaaagcaaga
     1201 caagccgaga tcacagggag tgggtcagac gtgcccgcga gttctccgaa atactcgctg
     1261 cttctggcag tgccttcgac tccagcgaga tcgctcgcgc cgaccgcgct ctcgtgatca
     1321 acggctggac gccaactggt ctcgcacaga cctgaccggg tataatcacc gtggcaggtg
     1381 tcgttgataa agctcgcgga gggatcatgt ccaggtctcg aaggacaata aagagttcgg
     1441 ccaggccggg tacggttaaa cgtagccgga tacgcgctgc cgcacgcgag atcaggcagg
     1501 agcgccagac cacctccggg cgtcagcatg cccgagcagg gtagaaccgc ccctccgggc
     1561 gcccactcct ccgtttcctt acaatagcct catggcagat ggggctacga ttacgatgtc
     1621 tttatcaact gcctcttcga ccacgagtat cgttcgatgc tctacgcgcc ggtcttcgtg
     1681 gtgcatgact gcggttttca ggctcgttgc gccctggaga tcgacgactc cgggagggtg
     1741 aggatcgaga acatcgtcga gctcattcag gggtgccgtt acggtatcca cgatatctcc
     1801 cgcaccgagc tcgataccga gaacaaactg cccaggttta acatgccgct ggagctcggc
     1861 ttgttcctcg gcgctaccag attcggaagc ccgaagcaga aaagaaaacg ctccctgatc
     1921 ctcgaccggg aaccataccg ctaccagaag tacatatcgg acatatctgg ccaggacata
     1981 aaatcccacg acggcgaccc aaagaccgct ataaggcagg tccgcgactg gctcaggaac
     2041 tccaccacca tgaccgacac catcatccca ggaccaggga gaataatcga gcgttacgag
     2101 ctcttccgcg aggagctccc cgagttgtgc cgccggtccc acctggaccc cgatgacctc
     2161 atctttaacg attacacaac ttgtgtctcg gagtggctca aagagaacga ctggtagccc
     2221 ctaccgcaac ggcaaagggg acgcgcaggc cgcaatcgaa agtccaggat catccatcgt
     2281 gttagccagg tgtctgggct ccggtcaggg ggcgtttgag atactcgccg gttggctggc
     2341 ctgtcgcctc tccatcggcg aagacagtct tgccgcgcag cagggttcgt acaggccagc
     2401 ctgcaagttc catcccctcg aacggcgtgt attcctgcgc cgatagcagc cgttccgttg
     2461 tgacgggatg cgtgtcctcc aggttgacga tggcgaggtc ggcgtcggcg ccgatggtta
     2521 tggttccctt gcgtggcgtg aggccgtagg cgcgggccgg gttggtggat acgagatcca
     2581 ctatgcgttc gagcgatgcg ccccgtctca ggccctcagt cagcatgaag gggtagatca
     2641 gggcggttcc cccgaagcct ggtagcgcct gccagaggtc tccttccttg ttctcctccg
     2701 agcagcaggc gtggtcagag cagacccagt cgatctcacc ccgcagtacg cccctccaca
     2761 gggcgtctac atctgactgc gccctgatcg gcgggttgac cttgccgcgc tggtcgttgt
     2821 acgtctcgta ggtgagtgcc agatggtgaa gcgtggtctc cagacgggcg tcgagagacg
     2881 gatgcttgcg tttgaggtcg agcgccgcct cgaacgcttc ctcgcttgag agatgtagca
     2941 ggttgaccgg gcatcgcgtg tgcccggcga gcacgccgac ctcggcgacg gcgaggtgtt
     3001 cggtcagcgt cgggcgagag gcgctgtagg cttcgagccc ttctagcacg ccttctcttt
     3061 gcacacgttc gttgaagatc cggatcaact ccggctgctc gcagtggatg gagagtgaga
     3121 cgcgggagcc atcgttgcgg ccagcctgga ccctggcgac ttcctccatg atctcgaaga
     3181 gatgcccgag gtcgtaggtc tcgctcatcg tgtagccagc cgcgtcgctc gatgcccccg
     3241 agagat
//
LOCUS       DATAIR010000550         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_130126, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000550 DATAIR010000000
VERSION     DATAIR010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130126"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..736)
                     /locus_tag="VHM69_13030"
     CDS             complement(386..736)
                     /locus_tag="VHM69_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741362.1"
                     /translation="MASYTVNRRGAAQARKLIKAKQYVLDSDWGDVQPKAASENAFLK
                     EHSWRDYSAWFLGLTDGAADDTKARYAFVYGDFRRLHRSGLIACLYRAAEWRHKEIER
                     AAYRLLQELDAATS"
     gene            1009..1446
                     /locus_tag="VHM69_13035"
     CDS             1009..1446
                     /locus_tag="VHM69_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741363.1"
                     /translation="MDEHENAFPMDFWAEDPSPHPWKFRPEGYLVVILSNAEEAHRAL
                     SSLVSQGFASRDVKLYTGQQILENYEVYAGRRNIADKVVGSVVSDSEGRELYLDYARQ
                     DRCAMWVRIPYVEDVPQALRVLADHDYVHTRYYGSKQQTDFNV"
BASE COUNT          290 a          473 c          498 g          275 t
ORIGIN      
        1 attccggcta taggggggac cacgtcgggt aagccgtggg acctcgcctg ccaggggtgg
       61 cctcctagtg cagagggcgg agggacccac caacccgact agtccattgg accccaacgc
      121 gggcagaccg tgctcgttcg aggcccgtcg gtcgagtagg gcatcggggg acgatcagga
      181 cggaacccgg gactctttcc gcgccccctc gcccaaggca caatggctca cggatggtca
      241 ccctgaggcg cctgctcagc aaggctcgat cgaaaggcga gatccgccgg aggcaggcat
      301 ggaaacaggc ggccgctgag ggggatcgcg atcgccgtca ccgcggcagc atctggagat
      361 ccggaaaggg cggatctcac ataggctagg aggtcgcggc gtcgagttcc tgtagcagcc
      421 ggtacgcggc tcgctcgatc tccttgtgac gccactcagc ggcccggtag aggcaggcga
      481 tcaagccgct tcggtgaagg cgccgaaagt caccgtagac gaacgcgtat cgcgccttgg
      541 tgtcgtcggc ggcgccatcg gtgagcccga ggaaccaggc cgagtagtcc cgccacgaat
      601 gctccttcag gaaggcgttc tcggacgcgg ccttcggctg aacatctccc cagtcgctgt
      661 ccaacacata ttgcttggcc ttgatgagct tgcgggcttg ggccgctccg cgtctgttca
      721 cggtgtacga agccatgaac gatccgtacc cagtcctgga ccatcaggaa ctcgagatca
      781 tcccgacgcc acccgtacag cgggttcact ccggcctgaa cgcccgaacc caaaccgaag
      841 tagcgacgct agcggcgggt tcgctctcat cattcgcgcg gtcgctgacg gtgggagggg
      901 tggaggtctc tcggcccacc atcagctgtg gtctggacgc tcgattgggc agctccgacg
      961 ggctgggtgg caaagacgta cattggatca tctcccgaag gaggcccgat ggacgagcat
     1021 gagaacgcgt tccccatgga cttctgggcc gaagaccctt cgccacatcc ctggaagttc
     1081 cggccggagg gctacctcgt cgtaattctc tcaaacgccg aagaggcaca tcgggcgttg
     1141 tcgtctcttg tcagccaggg gttcgcctcg cgagatgtca agctctacac cggccagcag
     1201 atcctagaga actatgaggt gtacgcgggt cggcgaaata tcgcggacaa ggtggtcggc
     1261 tcagtcgtgt ccgactccga gggtcgagag ctctacctgg actacgcccg tcaggatcgc
     1321 tgtgcgatgt gggtgcgcat cccgtacgta gaggatgtcc cgcaagctct acgagtcctc
     1381 gccgaccatg actacgttca tacgcgttac tacgggtcca agcaacagac cgacttcaac
     1441 gtctaggtgt tatggcggtc tcaggccgtg cttcaggccc ggtggggcgg gcaggacttg
     1501 aacctgcgac cgacggatta tgagtccgct gctctg
//
LOCUS       DATAIR010000551         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_62925, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000551 DATAIR010000000
VERSION     DATAIR010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62925"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VHM69_13040"
     CDS             <1..1474
                     /locus_tag="VHM69_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX2741364.1"
                     /translation="LFFFFGSPKEILSEYTALTGRSPVPPLWTFGLWMGRQTYSSEKE
                     ARDVAKKLRQERIPSDVIHLDTGWSEVPHRCDFEFSTSRFENPETMLADLEKDGFRVS
                     LWQLPYFNPKNRLHREATEKDLVILTASGEPPVDDAIIDLSKPEAEIWYREKLARLLE
                     MGVGIFTADFGEAAPLSGMYASKKGGFHEHNLYPLRYNMAVAGATEEVTGNGATFARS
                     AWAGSQRYPLHWGGDAENSDGGMLGTLRGGLSLGLCGFSFWSHFIGGFPYASPEDLYK
                     RWLAFGVLSSHSRCHGRPPTEPWEYGEEFTDDFRRTVEMRYRMMPYIYAQAAHASRQG
                     YPMLRTLFFEYPDDRTSWLVEDEYLFGTDLLVAPLFEEARSREVYLPPGLWTDYQSGE
                     TYEGARWHHVPAGEVPVVMLVRDGTAIPHVGLAQSTDRIDWSEIELRVFGAGDSAGGY
                     FCDPSDGELRFLRLSREADGFMLCEDPTDGRVNWEIRTAP"
     gene            1557..2060
                     /locus_tag="VHM69_13045"
     CDS             1557..2060
                     /locus_tag="VHM69_13045"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX2741365.1"
                     /translation="MSDGKPSEEIFEEYKQGEDIPLFSYALLAGVFNLAFAVFLLAAR
                     VTGRPIPERVDAKDIALLGMATHKLSLVGSQDAIMSPLRAPFTEVREKESPKKVDEKP
                     RGEGLRRSVGELLTCKFCLSVWLASFFTYGLVLVPRITRLVATVFAVVTVSDHLHQIY
                     KALMNRA"
     gene            2393..3181
                     /locus_tag="VHM69_13050"
     CDS             2393..3181
                     /locus_tag="VHM69_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741366.1"
                     /translation="MRFHDVWIGMSANVGEAGPVHDGGDRMGRVAQVHSAEKSGHSGV
                     SRLVIADDHPLFRIAIRQMVEDWPDLELVGEASDGMEIVEVCLRERPDLVLMDLWMPR
                     KNGFEATREIKRESPSTSVLVLTAFEEPDYLLEALKAGASGYVLKYMSPQQIAVAIRR
                     VLEGEFPLNQEVTMQLLVRLIEDSQNKRDGISPSLPMALSSREFEVLVLLARGQSNQQ
                     ISRELFISISTVKNHVHHIIEKLGVSDRVQAAVLAIERGWVTLR"
     gene            3573..5594
                     /locus_tag="VHM69_13055"
     CDS             3573..5594
                     /locus_tag="VHM69_13055"
                     /inference="COORDINATES: protein motif:HMM:NF039608.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741367.1"
                     /translation="MAWFSAVLGRRPLRVWAIVAVVGALAAATVLLIGMQAAQASHPE
                     VSLAGSDFEIDTNANLKVDDAAPSIDWASVNETRKADLASGSGDNSFGQGTKEDTAVP
                     TVVSGSIPPQKSDLLTFGVYLETTASGARFLNLFWHRVQEPNGTTNMDFEFNQSDEIS
                     GNGVTPVRTSGDLLIQYDLSRGGTSPTLFLSRWIDGSEGATAADCEASNSLPCWDTKD
                     NLTAAGDATGSINTSAILAADSDGLGAISARTFGEAQLDFDALTGGTDECVSFGSAYL
                     KSRSSDSFNSELKDFIAPENLNLNQCANVIIRKQTDPDEDPNTTQFGYTKTFPTDPAT
                     PDTFTLTDDGVQDYEGTVLPGTGYTVVEDVIPAGWDFVGVDCSASTGVDFSIAGATVT
                     FDIDANSDVLDCTYTNRARGTIVVEKITDEGNGAFDFTSNTLTPSPFTLTTTAAGSGG
                     KDSRTFDDLNPGTYDVAETVPAGWNLVSATCDDGSDPGSIGLSPGETVTCTFHDTRER
                     GAILITKTRKHAADGPGDHPHNGVEFTITGGSLPAGGTTVTTSGEGQACLDGLVLSSF
                     VGDYTVTETLPSGYQADGALAKTVTVSAESTCGDGNEAPVSFSNTPLTDISTSVDSQV
                     DGGTASTISCTDANGDTVASGSTGANGDGSATAEDLEPGTYTCEIVIDP"
BASE COUNT         1197 a         1696 c         1806 g         1068 t
ORIGIN      
        1 cctgttcttc tttttcggct cgccgaagga gatcctgtcg gagtacaccg cgcttacagg
       61 ccgctccccc gtccctcccc tgtggacttt cggactctgg atgggccgcc agacctactc
      121 ctccgaaaag gaggccaggg acgtggcgaa aaaactccgt caggagcgta ttccatcgga
      181 tgtgatccac cttgacacag gctggtccga ggttccccac cgctgcgact tcgagttttc
      241 gacctcgcgc ttcgagaacc cagaaacgat gctcgccgac ctcgaaaagg acggcttccg
      301 cgtcagcctg tggcaactgc cctacttcaa cccgaagaac agactgcacc gagaggccac
      361 agagaaggac ctcgtgatcc tcaccgccag cggcgaacct ccggtggatg acgccatcat
      421 cgacctgagc aagcccgagg ccgagatctg gtaccgcgag aagctggcaa ggttgctgga
      481 gatgggcgtc ggcatcttta cggcggactt cggcgaggcc gcgcctctct ccggcatgta
      541 cgcctcgaag aagggcggct tccacgagca caatctgtac cccctgcgct acaacatggc
      601 ggtcgccggg gctactgagg aggtcacggg caacggcgcg acctttgcgc gcagcgcctg
      661 ggcgggcagc cagcgttatc ctctgcactg gggaggagac gccgagaact cggacggagg
      721 gatgctcggg acgctgaggg gcgggctctc cctgggacta tgcggcttct cgttctggag
      781 tcactttatc gggggatttc catacgcgag cccggaggat ctctacaaac gctggctggc
      841 cttcggcgtg ctctcctcgc acagcaggtg ccacggcaga ccccccactg agccgtggga
      901 gtacggcgag gagttcaccg atgacttcag gagaaccgtg gagatgagat accgcatgat
      961 gccctatatc tatgcccagg ccgcccacgc ttcgcgccag ggctacccga tgctgcgcac
     1021 gctcttcttc gagtatcctg acgacaggac ctcatggctc gtggaggacg agtacctctt
     1081 cggcacggat ctcctggtgg cacccttgtt cgaggaggcc cgaagccgcg aagtctatct
     1141 gccgccgggg ctctggaccg actaccagag cggcgagact tatgaaggcg cccgctggca
     1201 ccacgtgccc gccggggagg tccctgtcgt gatgctcgtc agggacggca cggcgatccc
     1261 acacgtaggt ctcgcccaga gtactgatcg tatcgactgg agcgagatag agctgagagt
     1321 cttcggtgcc ggggattccg cagggggcta cttctgcgat ccctcggacg gcgagctgcg
     1381 cttcctgcgt ctctcacgcg aggccgatgg tttcatgctg tgcgaagacc ccacggatgg
     1441 gagggtgaac tgggagattc gtaccgctcc gtgagcctgc cgcggatgtt tagggaccat
     1501 gatgcgaggg ggatatccac tcagagcgga taagcatagt tgcgcaagga gcacgcatga
     1561 gcgacggcaa gccgtccgag gagatcttcg aggagtacaa gcagggcgaa gacataccgc
     1621 tgttctcgta cgctctgctg gccggggttt tcaacctggc cttcgctgtc ttccttctgg
     1681 cggccagggt cacgggcagg cccataccgg aacgggtcga tgcgaaggac attgcgctcc
     1741 tcggcatggc gacccacaag ttgagcctgg tgggctcgca ggatgcgatc atgagccccc
     1801 tgcgcgcccc gttcacagag gtcagggaga aggagagtcc caagaaggtc gacgagaagc
     1861 caaggggcga aggcttgcga aggtccgtgg gcgagctcct gacgtgcaag ttctgtctaa
     1921 gcgtgtggct tgcctccttc ttcacctacg gactcgtgct cgtgccgcgc atcacccgtc
     1981 tcgtggccac cgtgttcgcc gtagtcaccg tctcggatca cttgcaccag atatacaagg
     2041 ccctgatgaa ccgggcctga gggcgttcac aaacctctga gtgcggcgac cagataatcg
     2101 aaatagccgg agccgtacgc cggcacagag ggtgcagatc actccgactg acccggcaac
     2161 ctgcttgccg cacgctacac gagcgatcat ttgtacctta tgacactaag tgtcgaagga
     2221 tacagacacc cgcggattcc tctggctcta tgatgatcag ggagagcaat aggaaggcat
     2281 ggcagggtga gaaccggcgc gggagagccg tggcgcaggt catagtactg ttcgtgctgc
     2341 cttcgtccct gtcgttgccg gtctacaggc aaggtgaaca ggacggaaga acatgaggtt
     2401 tcacgacgta tggatcggta tgagtgccaa tgtgggcgag gccgggcccg tccatgatgg
     2461 tggggaccga atgggaaggg tcgcacaggt tcacagcgcc gagaagagcg gacattccgg
     2521 ggtttcgagg cttgtcatag ccgacgatca tcccctcttt cgaatcgcga tcagacagat
     2581 ggtggaggac tggccggatc tggagcttgt tggcgaggca tccgacggca tggagatcgt
     2641 agaggtctgt ctgcgcgagc ggccagatct tgttttgatg gacctgtgga tgccaaggaa
     2701 aaacgggttc gaagccaccc gtgagatcaa acgagaatca ccctccacca gcgtgcttgt
     2761 gctgaccgcg ttcgaggagc ctgactacct gttggaggcg ttgaaagctg gggcctcagg
     2821 ttacgtgctc aagtatatga gtcctcaaca gatagcagtg gctatcagga gggtgcttga
     2881 aggcgaattc ccattgaacc aggaagtcac catgcaactg cttgtgcgcc tgatcgaaga
     2941 cagtcaaaat aagagggatg ggatttctcc gtcccttccc atggcgcttt catcgcgcga
     3001 gttcgaggta ctcgtcctgc tggcgcgagg gcaaagcaac cagcagatct ctagagaact
     3061 gtttatcagt ataagcactg tgaagaatca cgtgcatcac atcatcgaga aactaggggt
     3121 ctcggaccgt gtgcaagcgg ccgttctggc aattgaacga ggttgggtca ctctccgata
     3181 ggttttcaaa tgcggcatgg gccggtagat ccacgaaaat agatccctcg tcaccaggaa
     3241 tagatccacc gatccatata caggttcttc atctgagttc atccttctaa acgttcgtag
     3301 caatagctgc gaatggggcg agatccttgc ctgacgaggt agggtccttg ctccctacta
     3361 gccaggggga aggaggcgga gatacggtta gcttaagcgg gactcgaact gccaaggggc
     3421 cggatgcttg ttttcgagta ggcaccgata caagaaagac ccgaccccgt aagcattcta
     3481 ctaccctgat ccgcctacta ctgacttctc tggcggtgaa gggaaagggg aaacagaatg
     3541 caggatcgat gtacctcctt taggaggacg tgatggcgtg gtttagcgcg gtactcgggc
     3601 gacgcccgct cagagtttgg gcgatcgtcg cggtagtagg ggctctcgct gcggcgacgg
     3661 tgctcttgat tggcatgcag gctgcacagg ccagtcaccc tgaggtgagc ctagcgggca
     3721 gcgacttcga gatcgacacc aatgccaacc tcaaggtaga cgacgcagcg ccgtccatcg
     3781 actgggcgag cgtcaacgag acgaggaagg cggaccttgc ctctggctct ggagataact
     3841 ctttcggaca aggcaccaag gaggacacgg ccgttccgac cgtggtgagc gggagcatcc
     3901 caccgcaaaa gagcgacctg ctcaccttcg gcgtctacct ggagacgacc gccagtggcg
     3961 cccgcttcct gaacctcttc tggcatcggg tgcaggagcc aaacggcacg acgaacatgg
     4021 acttcgagtt caaccagtca gatgagatct cgggcaacgg cgtgacaccg gtgcgcacct
     4081 ctggtgactt gctgatccag tacgacctct ccaggggcgg cacgagcccg accctgttcc
     4141 tctccaggtg gatagacggc tctgagggtg ccacagccgc tgactgcgag gcttcgaact
     4201 cgctgccgtg ctgggacacg aaggataacc tcaccgctgc gggcgacgcc acggggtcga
     4261 tcaacacctc ggccattctc gctgcggact ccgacgggct gggagccatc tcggcgcgca
     4321 ccttcggcga ggcacagctt gacttcgacg cccttaccgg cggtacggac gagtgcgtct
     4381 cgttcggcag cgcctacctc aagagccgct cttcggactc tttcaattcg gagctgaagg
     4441 acttcatcgc gccggagaac ctgaacctca accagtgcgc caacgtgatc atccggaagc
     4501 agaccgatcc ggatgaggac cccaatacaa cgcagttcgg ctataccaag acgttcccta
     4561 cggaccccgc gaccccggac actttcacgt taacggatga cggtgtgcag gactacgagg
     4621 ggaccgtact gccaggcacg ggttacacgg tggtggagga cgtcatcccc gcgggctggg
     4681 acttcgtcgg cgtcgactgt agcgcgagta cgggggtgga cttcagcatc gccggcgcca
     4741 cggtgacctt cgacatcgac gccaactccg acgtccttga ctgtacgtac accaacaggg
     4801 cgcgcggcac gatagtcgtc gagaagatca ctgacgaagg caatggtgct ttcgacttca
     4861 cgtcgaatac gctaacgccg agtcctttca ccctgacaac gacagcagcg gggtcaggcg
     4921 gcaaagactc acgcaccttc gacgatctca atccaggcac ctatgacgtc gccgagacgg
     4981 tgcccgcggg ctggaacctg gtttcagcga cctgcgatga tgggagcgat cccggctcaa
     5041 tcggcctctc gccaggtgag acggtaacgt gtacgttcca cgacacgcgt gagaggggcg
     5101 cgatcctgat caccaagacc cgcaagcacg ctgcggatgg acctggtgac cacccgcaca
     5161 acggggtgga gttcacgatc acaggtggtt cgttgccggc aggcggcacg acggtgacca
     5221 cgagcggtga aggtcaagcg tgtctcgacg gtctcgttct cagcagcttt gtcggggact
     5281 acaccgtcac cgagacattg ccgagcggct accaagcgga cggagctttg gcgaagaccg
     5341 tgaccgtgtc ggctgagtcg acgtgtggtg acggcaatga ggcgcccgtc agcttcagca
     5401 acacaccgct gactgacatc agcacgtccg tcgactcgca ggtggacggc ggcacggcat
     5461 cgacgatctc gtgcacggac gcgaacggcg acaccgtcgc ctccggatct acaggcgcca
     5521 atggtgacgg gtcggccacc gcggaggacc tggagccagg cacctacacg tgcgagattg
     5581 tgatcgaccc gtaaactcaa gaagggagac tatctcgaga gggtgggacg agtcccaccc
     5641 tctcatcacg tgcatcatcg agaagctcgg gggctcggat cgcgtgcaag cggccgttct
     5701 ggctatagaa agcggaagcc cactccctag agtgcgttgc gcgggctggt agacacgaat
     5761 atccctc
//
LOCUS       DATAIR010000552         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_155823, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000552 DATAIR010000000
VERSION     DATAIR010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155823"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VHM69_13060"
     CDS             complement(<1..522)
                     /locus_tag="VHM69_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2741368.1"
                     /translation="MAKPVTMPQLGESVTEGTITRWLKAEGDEVTIDEPLAEVDTDKV
                     NAELPSPVAGTIEKFLVSEGSTVDVGTELVLIAVDGEAESPSTEAPPQENVADDAPTE
                     EFPASDTEAQPAVQESEKPRAGARGDGNGRGTVEDAETLRLTRSSPVVRRLAEEHGVE
                     IADVPGTGTEGRVT"
     gene            complement(535..1305)
                     /locus_tag="VHM69_13065"
     CDS             complement(535..1305)
                     /locus_tag="VHM69_13065"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741369.1"
                     /translation="MDVRGYNQEAWDREVERGNQWTVPFGPDVIEAARRGEWTVLLTD
                     SVPVPKSWFPEMGGADVLCLASGGGQQAPIFAAAGANVTVLDNSPKQLAQDRLVAGRE
                     SLDLKTVEGDMRDLSSFADESFDLIFHPVSNLFVPEVRPVWTEAFRVLRRGGILLAGF
                     LNPAVYIFDLELADSTGELRVKYKLPYGAATSLSEEELRAQVERGDPLEFSHTLEDQI
                     GGQIEAGFLISGFYEDRHRDDPIAAYMPTFVATRATKP"
     gene            complement(1306..2292)
                     /locus_tag="VHM69_13070"
     CDS             complement(1306..2292)
                     /locus_tag="VHM69_13070"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2741370.1"
                     /translation="MATKNLLQAIHEGLAEEMRADESVMVLGEDVGRAGGVFRVTQGL
                     QEEFGEQRVMDTPLAESLIVGSAIGLSVNGMHPVVEIQFADFIPPAFDQIVNEAAKFY
                     YRSNGAWTCPITIRAPYGVVPGGALYHSQSIEAWFCNTPGLKVVAPTYPADAKAMIKS
                     SIRDPNPVLFLEHKRTYRLIKEEVPDEDYEVPLGKARVHREGADITVVAYGLMLYVVL
                     ETAGKLSEEHGIETEVVEPRTLYPLDKETILDSAKKTGKVLVISEANLTGSVSGEIAA
                     IVAREAFEWLDGPVMRLGAPDVPAAAFAKPMMDYFVPSAEKIEEAMLELARY"
     gene            complement(2295..3299)
                     /locus_tag="VHM69_13075"
     CDS             complement(2295..3299)
                     /locus_tag="VHM69_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2741371.1"
                     /translation="MAIRETSARHEALGLDEDDLRRMHYHMLIARRVDERSWILNRQG
                     KAAFVISCQGQEGAQVGAAYNLRPGYDYLYPYYRDSGMTLLLGQTPRDQLLSLLGRKE
                     DPNSGGRQMPGHYSSRKLNIVTASAPVGVQFPQATGTALAFKLRGEDGVVLACGGEAS
                     TSGGDWHEAMNFAGIHDLPVVFLVENNVYAISVPAKFQVAGSIARRAEGYGFPGVEVD
                     GNDVLDVYEVAREAFERARRGEGPTLIEARTYRMTAHSSDDDDRRYREREEIEEWRQK
                     DPIVRFEKYLMEHDLLDEERKEEMDAEIKAEVAEASEYAENAPFADPEDVLTGVYAER
                     "
     gene            complement(3390..3704)
                     /locus_tag="VHM69_13080"
     CDS             complement(3390..3704)
                     /locus_tag="VHM69_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741372.1"
                     /translation="MHARVVNLRVRAVDTSEMVRIYRASVMPALRPQRGFDGALLLSD
                     PETGICISITMWETEEDREAGETSGFYKEQIAKFADLLIETPVRKHYDVSVLEQIREL
                     AG"
BASE COUNT          686 a         1351 c         1175 g          814 t
ORIGIN      
        1 ggttacgcgt ccctcggtgc ctgtgccggg gacgtcagct atctctacgc cgtgttcctc
       61 ggcgaggcgc cgaacgacgg gcgaggagcg cgtgaggcgc aaggtctcgg catcctcgac
      121 cgtaccgcgc ccgttgccgt caccccgggc gccggcgcgt ggcttctcac tctcctggac
      181 ggcgggctgg gcctcggtat ccgaagcggg gaactcttct gttggagcat cgtcggcgac
      241 gttctcttgt ggcggggctt ccgtggaggg agactcggct tctccgtcga cggcgatcag
      301 gacgagctcg gtcccaacgt cgaccgtcga gccctcggag acgaggaact tctctatagt
      361 accagccacg ggagagggta actcggcgtt gaccttgtcc gtatccactt cggcgagggg
      421 ctcgtctatc gtcacctcat caccctcggc tttcagccag cgggtaatag tcccctccgt
      481 aacgctctca ccgagttgcg gcatcgttac cggttttgcc acgattccct cctgctatgg
      541 ctttgtcgcc cgcgtggcga cgaaggtcgg catataggct gcgatcgggt cgtcgcggtg
      601 gcgatcttcg tagaagcctg aaatcaagaa tccagcctct atctggcccc cgatctgatc
      661 ctccagggtg tggctgaact cgagcggatc tcccctctcg acctgcgccc tcaactcctc
      721 ttcgctcagg ctcgtcgcgg cgccgtacgg caacttgtac ttgacccgca actcacctgt
      781 actgtcggcg agttcgaggt cgaagatgta gaccgcaggg ttcaggaaac cggccaatag
      841 aattccccca cgacgcagga cgcggaatgc ctcggtccat accggcctga cctcgggcac
      901 gaaaaggttg gagacggggt ggaagatgag atcaaagctc tcgtcggcaa acgacgatag
      961 gtccctcatg tccccctcga cagtcttcag gtcgagcgac tcgcgcccgg cgacgagcct
     1021 gtcctgcgcg agctgcttcg gcgagttgtc gaggacggtc acgttggcac cagccgcagc
     1081 gaagatagga gcctgctgcc cgccgcccga ggcgaggcag agtacgtcgg cgcctcccat
     1141 ctcggggaac catgacttcg gcacaggaac gctgtccgtc aacaatacgg tccattcgcc
     1201 gcgcctagcc gcctctatga cgtcggggcc gaagggtacg gtccactggt tcccccgttc
     1261 gacctccctg tcccacgcct cctggttgta tccccgcaca tccatctagt agcgcgccag
     1321 ctcgagcatt gcttcctcta tcttctccgc cgagggcacg aagtagtcca tcatcggctt
     1381 cgcgaaagcc gccgcaggca cgtcgggagc gccgaggcgc atcacgggcc cgtcgagcca
     1441 ctcgaacgcc tcccgagcca cgatggcggc gatctcacca gagaccgaac cggtcaggtt
     1501 ggcctcggag atcacgagca ccttgcccgt cttcttcgcc gagtcgagga tggtctcctt
     1561 atccagcgga tacagcgtcc tcggctcgac cacctcggtc tcgatgccgt gctcttcgga
     1621 gagcttaccc gcagtctcga ggaccacgta gagcatgagg ccgtaggcga ctacggtgat
     1681 gtcagcgccc tcgcggtgga cgcgggcctt tccgagagga acctcgtagt cctcatccgg
     1741 cacttcctct tttatgaggc ggtaggtacg tttgtgttcg aggaagagga cggggttcgg
     1801 gtcgcggatc gagcttttga tcatggcctt cgcgtccgcc ggatacgtcg gcgcgactac
     1861 cttgagaccc ggagtgttgc aaaaccaggc ctcgatgctc tgcgaatggt acagagcccc
     1921 gccaggcact acgccatacg gcgcgcggat ggtgattggg caagtccagg cgccgttgga
     1981 acgatagtag aacttggcgg cctcgttgac gatctggtcg aacgccggag ggatgaagtc
     2041 ggcgaattgt atctctacca cggggtgcat accgttcacc gagagtccta ttgcggaacc
     2101 cacgatcagg ctctccgcca gcggcgtgtc catgaccctc tgttccccga actcttcctg
     2161 cagtccctgc gtcaccctga atacaccacc ggcccggcca acgtcctcgc cgagtaccat
     2221 gaccgactcg tccgcgcgca tctcctcggc gagtccctcg tggatggcct ggagcaaatt
     2281 cttcgtcgcc atgactatcg ctccgcgtag acgccggtca gcacgtcttc gggatccgcg
     2341 aacggagcgt tctccgcgta ctcgctggct tcggcgacct ccgcctttat ctcggcgtcc
     2401 atctcctctt tccgctcctc atccaggaga tcatgctcca tcaaatactt ctcgaaccgg
     2461 acgatgggat ccttctgacg ccactcctcg atctcctcgc gctcccgata ccgccggtcg
     2521 tcgtcgtccg atgagtgggc ggtcatgcgg tatgtcctgg cctcgattag cgtcggtcct
     2581 tcaccgcgcc gggcgcgctc gaaagcctct cttgctacct cgtatacatc gagcacgtcg
     2641 ttgccgtcta cctcgacccc ggggaagccg tagccctcgg cacgcctcgc tatggacccc
     2701 gcgacctgga acttggctgg gacggagatg gcgtagacgt tgttctcgac gaggaaaacc
     2761 acgggcagat cgtggatgcc cgcgaagttc atagcctcgt gccagtcgcc gccgctggtc
     2821 gaagcctctc cgccacacgc gagcacaacg ccgtcctcgc cgcgcagctt gaacgcgagg
     2881 gccgtgcccg tcgcctgcgg aaactgcacc ccgacggggg ccgaagcggt cacgatgttc
     2941 agcttgcggc tgctgtagtg accgggcatc tggcgaccgc cgctgttcgg gtcttccttc
     3001 ctgccgagga ggctcaggag ctggtcgcgg ggcgtctggc cgaggagcag ggtcatcccg
     3061 gagtcgcggt agtatgggta gagataatcg tagcccgggc gcagattgta ggcggcgccg
     3121 acctgggctc cctcctgtcc ctggcaggag atcacgaacg ccgccttgcc ctggcggttc
     3181 aggatccagg accgctcgtc caccctgcgc gcgataagca tgtggtagtg catcctgcgc
     3241 aggtcgtcct cgtccaaccc cagagcctcg tgcctcgcac tggtctcccg gatagccata
     3301 gacctcctcc taagctccgc tttccctcac ggagaagcag gtaccgctcc tcgaatcctt
     3361 atcgtatcag aacgcaactc cccgaaccgt taccccgcca gctcccttat ctgctccaga
     3421 acgctgacgt cgtaatgctt ccttacggga gtctcgatca ggaggtcagc gaacttggcg
     3481 atctgctcct tgtagaaacc gctggtctcg cccgcctccc tgtcctcctc ggtctcccac
     3541 atcgttatcg agatgcatat gccagtctca gggtcactca ataacagagc cccgtcgaag
     3601 ccccgttgcg gcctgagagc cggcatgacc gaagcccggt agatgcggac catctcgctg
     3661 gtgtctaccg ccctgacccg aaggttcacc accctggcgt gcattctcga ctccttcccg
     3721 aactccacaa cacgtgatac tcaagtatca acttaattat atatcagcgc cgcctccctg
     3781 cggtcggcgg ctttcagcac ggcgcttcgc gcctttcagc tcgcttcgtg ccttatcggc
     3841 cctcgctttc agctgtcagc tcttcctcgg atggcgtcgc tcacgctcat gaggcgaaat
     3901 ccatcgggca ggagctgctc ttctcgaacg aagatctcgg catccacgcc cttgctgaaa
     3961 gctgatggct gaaagcggag gcgtagccgt agcgtgctga aagcgaagcc catagggcgt
     4021 agcgtg
//
LOCUS       DATAIR010000553         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_130211, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000553 DATAIR010000000
VERSION     DATAIR010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130211"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1071
                     /locus_tag="VHM69_13085"
     CDS             13..1071
                     /locus_tag="VHM69_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX2741373.1"
                     /translation="MRDASYDFLKRLLSTPGPSGYEEAAAKIWREEAGKFAEVYGDRM
                     GNSFATIGAGGSPKIMLAGHIDEIGVMVTHVDEKGLLRFTGVGGWDSQVLVGQRVRLL
                     TKSGEVAGVIGKKAIHVMEADERKKVSEIKSLWIDIGANDADEAKGMVRVGDVGVIDA
                     DLVELPNGRIASRSVDNRMGAFVVLEAVRLLSEEEDIGAEVVAVACVQEEIGLYGARG
                     AAFGLDPDAAIAVDVTHATDTPGVSKNEHGDHSLGSGPVIKRATNISPIVSEGLIAAA
                     DSENIPYTLEADSRSTGTDADAIQFTRAGISTGLISCPNRYMHSPNEVVDIEDVENCA
                     RVIAAYIKSLNAETTFVR"
     gene            1090..1977
                     /locus_tag="VHM69_13090"
     CDS             1090..1977
                     /locus_tag="VHM69_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2741374.1"
                     /translation="MREEPKRRASTPVEAALLLTVFFFGTNFTAVKIVVESVPPILFA
                     ATRFTLAGLLLLFLLRFVEQGIRLRRADFLAVLGLGVVGITLTQTVFTLGVSLTTAAN
                     TALVYSTAPVWGMLLGFALGLERPRLAGILGIGLSLVGVGFIVYGGLEFAGTSLVGDA
                     LILVAAVFWGSYTMLSLSLLQRYSPLALAAYAMTLGGLAAFPLAALDPGGFHVGALDG
                     LTWGAAAYSTLFSSAFGFAAWGWGISHVGANRVLIYQYLVTLIGVTTGIVLLGEDFGL
                     GQLIGAVVILCGVYLARRK"
     gene            2060..2269
                     /locus_tag="VHM69_13095"
     CDS             2060..2269
                     /locus_tag="VHM69_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX2741375.1"
                     /translation="METVRVSTKYQVVIPKAVREAAGIRPGQEVAVLRYGGRIELIPL
                     RPVGEMRGFLRGLDTTIEREREREV"
     gene            2271..2660
                     /locus_tag="VHM69_13100"
     CDS             2271..2660
                     /locus_tag="VHM69_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2741376.1"
                     /translation="MPAKNVVDSSGWLEYFADGPNADFFEEAIEEEASLVIPSISIYE
                     VFKRLLAEPDGEEAGFLVVAAMQRGEVVDLDAELALDAARISRETRLPMADSIILATA
                     HARDARLWTQDADFEGIEGVEYVRKQK"
     gene            2714..2932
                     /locus_tag="VHM69_13105"
     CDS             2714..2932
                     /locus_tag="VHM69_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741377.1"
                     /translation="MTEVSGGSWRRWRRRGTTIPSKVGEFGMPEPEFEFVTADDRVLP
                     GKVAGLVSDAYMGEGPSHEGPWTFLFTL"
     gene            complement(2922..3581)
                     /locus_tag="VHM69_13110"
     CDS             complement(2922..3581)
                     /locus_tag="VHM69_13110"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX2741378.1"
                     /translation="MTTSGGQDGNTGRIEAFSDGVFAIAITLLIIEIGVPHAEGTESL
                     FEKLVELWPSYLGYAISFLVIGTVWANHHNRFRLISRSDHVLLFLNILFLMCVAFVPF
                     PTALLAEYIREEEHRITAVAVYSGTLAVTAVFFTLLWLYAAGNYRLIDRSVDPSLLRA
                     MTRRFVLGMLLYVLAFAIAFVSAATSLTLIVILALIFVLPEPDSSRRSARRTRRRSFR
                     E"
BASE COUNT          733 a         1161 c         1273 g          729 t
ORIGIN      
        1 gaaaggactc atatgaggga cgcgtcgtac gatttcttga agaggctgct ctcgacgcct
       61 ggaccgagcg gctacgagga ggccgccgcg aagatctgga gggaggaggc agggaagttc
      121 gccgaagtct acggtgacag gatgggcaac tccttcgcca cgataggcgc gggcggttcg
      181 ccgaagatca tgctcgctgg gcacatcgat gagatcgggg tgatggtcac ccacgttgac
      241 gagaaaggcc tgctccgctt cacgggcgtg ggcggctggg actcgcaggt gctcgtcgga
      301 cagcgggtca ggttgctgac gaagagcggc gaggttgcag gtgtgatcgg caagaaggcc
      361 atccacgtca tggaggcgga cgagcgcaag aaggtctccg agataaagag cctctggatc
      421 gacataggcg ccaacgacgc cgacgaggcg aagggcatgg tgcgcgtcgg ggacgttggc
      481 gttatcgacg cggacctggt ggagcttccg aacggacgca tcgcctcccg ctccgtagac
      541 aaccgcatgg gcgcgttcgt ggtgctcgaa gccgtgcgcc ttctctccga ggaagaagat
      601 atcggggccg aggtcgtcgc cgtggcctgc gtgcaggagg agataggtct ctacggcgcc
      661 cgcggggccg ccttcggcct cgaccccgac gccgccatcg ccgtcgacgt gacccacgcg
      721 acggacaccc cgggcgtctc caagaacgag cacggagatc actccttagg cagcggcccc
      781 gtcataaagc gggcgacgaa catctcaccc atcgtctccg agggacttat cgccgctgct
      841 gattcggaga acatcccata caccctggag gccgattcgc gctcgacggg gacggacgct
      901 gacgccatac agttcacgag ggcagggatc tcgaccggac tcatctcctg cccgaaccgc
      961 tatatgcact ccccgaacga ggtcgtcgac atcgaggacg tcgagaactg cgcgcgtgtg
     1021 atcgccgcct acataaagtc cctgaacgca gaaacgacct tcgtccgctg acccaggctc
     1081 cccgtgaccg tgagagagga accgaagcgg cgtgcctcga cccccgtcga ggctgcgctg
     1141 ctcctgacgg tcttcttctt cggtacgaac ttcaccgcgg tcaagatcgt cgtcgagagc
     1201 gttccgccga tactgttcgc ggcaactcga tttaccctgg ccggtctcct gctcctgttt
     1261 ctcctgcgct tcgtggaaca gggcatccgc ctgcgccgcg ccgacttcct cgccgtgctc
     1321 ggcctcggag tagtcggcat caccctgacc cagacggtct tcacgctcgg ggtgagcctg
     1381 acgacagcgg cgaatacagc cctcgtgtac tcgacggccc ctgtgtgggg gatgctcctc
     1441 ggtttcgcac tcggcctgga gaggccgaga ctcgcgggta tcctcggtat agggctctcc
     1501 ctcgtcgggg tcggcttcat cgtctacggc gggctcgagt tcgccggcac gagcctcgtc
     1561 ggtgacgccc tcatcctcgt cgcggccgtg ttctggggct catataccat gctctccctc
     1621 tcgctcctgc agcgctactc tcccttggcc ctcgcagcct acgccatgac cctgggcggc
     1681 ctcgccgcct tcccgctcgc agccctcgat ccgggcggct tccacgtagg cgccctcgac
     1741 ggcctgacgt ggggcgctgc ggcctactca accctcttct cttcggcctt cggcttcgca
     1801 gcctggggat ggggcatctc ccacgtcggt gctaacaggg tcctgatcta ccagtacctc
     1861 gtcaccctga tcggcgtgac cacaggcatc gtcctgctcg gcgaagactt cggattaggc
     1921 cagctcatcg gcgccgtcgt catcctctgc ggcgtctacc tggcacgaag gaagtagtct
     1981 ccaacgctcg tcgttaggca tacgcagtgt gatacgatcc gatcgcgtat tacattatct
     2041 ctatcgatgg agaaagcgta tggagacggt tagagtatcg accaagtacc aggtggtcat
     2101 cccgaaggct gtgagggaag cggcgggcat acgtccgggg caggaggtag ctgtgctgcg
     2161 ctacggtgga cgcatagagt tgatcccgct ccgtccggtc ggcgagatgc gagggttctt
     2221 gcgtgggctt gacacgacca tcgagcggga gagggaacgg gaggtctgag gtgcccgcca
     2281 agaacgtggt cgattcctca ggctggctcg agtacttcgc cgacgggccg aatgctgact
     2341 ttttcgagga agctatcgag gaagaggcct cgttggttat accttcgatc agcatctacg
     2401 aagtattcaa gcgcctcctc gccgagccgg acggcgagga agccgggttc ctggtcgtcg
     2461 cggcgatgca gcggggggag gttgtcgacc tcgacgcgga gcttgcgctc gacgctgcca
     2521 gaatatccag agagacgagg ctccccatgg ccgacagcat tatcctcgcc acggcacatg
     2581 ccagggacgc caggctctgg acccaggacg cagacttcga gggtatcgaa ggcgtcgaat
     2641 acgttcgtaa acagaaatag acgtacgtag tttgcccttt ggcggatgcc ccggagaggc
     2701 aggtcgagac atcgtgacgg aggtaagcgg tggatcgtgg cgacgctgga ggaggcgcgg
     2761 gacgacgatc ccgtcgaagg taggcgagtt cgggatgccc gagccggaat tcgagtttgt
     2821 gaccgctgat gaccgcgtcc ttcccgggaa ggtggcgggt ctggtctccg acgcttacat
     2881 gggcgaggga ccgtcccacg aaggtccctg gaccttcctg ttcactctct gaaggagcgc
     2941 ctgcgggtgc gacgggcgct tcttctggaa ctgtccggtt cgggcagcac gaagatgagg
     3001 gcgaggatca cgatgagcgt caggctcgtc gccgcgctga cgaaggcgat cgcgaaggcc
     3061 aggacgtaga ggagcatgcc gaggacgaag cggcgcgtca tggcgcgcag cagggaagga
     3121 tcgacgctcc tatctatcag cctgtagtta ccggcggcgt agagccagag cagggtgaag
     3181 aagacggcgg tgacggccag cgtgccgctg tagactgcga cggcggtaat tcgatgctct
     3241 tcttccctga tgtactcggc gagcagggct gttgggaagg ggacaaacgc gacgcacatc
     3301 aagaacagga tgttcaggaa gagcaggacg tggtcggagc gtgagatcag gcggaatcgg
     3361 ttgtggtggt tcgcccagac ggtgccgatg acgaggaagc tgatcgcata cccaagataa
     3421 gagggccaca actcgacgag cttttcgaat agagattccg tgccttcggc atgaggtact
     3481 cctatctcga tgatcaggag tgtgatcgcg atggcgaaca ctccgtcgga gaaggcctct
     3541 atccttcctg tgttgccgtc ctgtccgccg ctcgtagtca ccggggctcc ctcttcgtcc
     3601 ctgcccggta gtttgtatca cccgcgagta caggcgcaat gcccgaggtc ctcccggatc
     3661 cgcaagctca caggctgagt cgcccaacaa gtcgcttgcc ttccggggtg caaatctggt
     3721 gtaataccgg cgacagtaga tcgaccaggg agaggaaaat gcgccaggct ttcatgtcta
     3781 tccaggaggt aattgacacc tatgtagagg acatgaagct ccatgagcac tttgaacctt
     3841 gggattttgg cgcatgtaga cgccggtaag acgaccctga ccgagcggct gctcta
//
LOCUS       DATAIR010000554         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_339538, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000554 DATAIR010000000
VERSION     DATAIR010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339538"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VHM69_13115"
     CDS             complement(<1..1276)
                     /locus_tag="VHM69_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX2741379.1"
                     /translation="MSDSLRRYVIWVVVALVGAVCWGMLALSRGETINAGWLLFAALA
                     SYAIAFRFYARFIQRKVLETDDSRATPAERLNNGRDFEPMDKRVLFGHHFAAIAGAGP
                     LVGPVLAAQMGFLPGTVWIIVGVILAGAVQDMTTLFFSMRRDGKSLGQMAREEIGPVG
                     GAAALIAVLSIMVILLAVLALVVVLALAESPWGSFSLAMTLPIALLMGVYLRFLRPGR
                     VLEVSLIGLVLLLLAILGGGWISESSAYADAWTFTPNQIVFALIAYGFVASVLPVWLL
                     LAPRDYLSTFMKIGTIALLAVGIALTLPVMQMPSTTEFAFNGQGPVFAGALFPFVFIT
                     IACGALSGFHSLVASGTTPKLIQKESQVRVIGYGAMLTESFVAVMAMIAACILDPGVY
                     FAMNANPALLGDTAQSAAQAVSGFGFSVNAETL"
     gene            complement(1465..>1776)
                     /locus_tag="VHM69_13120"
     CDS             complement(1465..>1776)
                     /locus_tag="VHM69_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2741380.1"
                     /translation="ANCIGVFIANFTSSPADVLLYATAILFPLIYLSGFFRNQHPTGI
                     LQTLRGFVPFAYTDDALKTVFGLTTGTTPLEILFYPTVVCAVLAGAVCMTAPHLLSQR
                     P"
BASE COUNT          380 a          530 c          596 g          270 t
ORIGIN      
        1 tgagagtctc ggcatttacc gagaaaccga agccgctcac ggcctgggcc gccgactggg
       61 cggtgtcgcc gaggagtgca gggttggcgt tcatggcgaa gtacacgccg gggtccagga
      121 tgcacgctgc gatcatggcc atcacagcca cgaacgactc cgtgagcatc gccccatagc
      181 cgatcacgcg cacctgcgac tctttctgga tcaacttggg cgtcgtcccc gaagccacga
      241 gcgagtggaa gccggagagt gccccgcacg ctatcgtgat aaacacgaac gggaagaggg
      301 cgcctgcgaa cacgggcccc tggccgttga atgcgaattc cgtggtgctg ggcatctgca
      361 tgactggcag tgttagcgcg atgcccacgg cgagcagggc gatggtcccg atcttcatga
      421 aggtcgagag gtagtcgcgc ggggccagaa gtagccacac gggaagtacc gaggcgacga
      481 agccgtaggc gatgagggcg aagacgatct ggttgggtgt gaacgtccag gcgtcggcgt
      541 aggctgagga ctcggagatc caaccacccc cgaggatcgc gagcagcagg agcacgaggc
      601 ctatgagcga gacttcgagc acccttccag gacgcaagaa gcgcagatac acacccatga
      661 gcagcgctat ggggagggtc atcgcgagcg agaacgatcc ccacggcgac tcggcgagcg
      721 cgaggacgac gacgagcgcg agcacagcga gcaggatgac catgatggag agcacggcga
      781 tgagcgccgc cgccccgccg acgggtccga tctcctccct cgccatctgc cccagggact
      841 tgccgtctct tcgcatggag aagaagagcg tggtcatgtc ctgcacggcg cctgccagta
      901 tgacgccgac gatgatccag acggtccccg gcaggaaccc catctgagcc gccagaacag
      961 gcccgacgag cggtcccgcc cccgctatgg ctgcgaagtg atgcccgaag agcaccctct
     1021 tgtccatcgg ctcgaagtcg cggccattgt tgagccgctc ggcaggcgtc gccctcgagt
     1081 cgtcggtctc gagcaccttt ctctggatga agcgggcgta gaacctgaag gcgatggcgt
     1141 atgaggcgag agccgcgaag aggagccaac ctgcgttgat ggtctcaccg cgtgagaggg
     1201 caagcatccc ccagcagacc gccccgacga gggcgacgac gacccagatc acgtaccgac
     1261 gcagactatc actcataggt cctcctacct aaagtgccct tccctaaggc tcttacctaa
     1321 gcatatgcat cctatcatag cggaaaagca tatcgaacgc gggaacccgc tgccagattg
     1381 gagaaggccg ccttgtacca gcgaaccgga ggatgctgtc gtctcctcca cgcctgaacg
     1441 acaggaggct atttccgacg ggctctacgg acgttgggag aggagatgtg gggcggtcat
     1501 gcagaccgcg ccggccagga cggcgcagac gacggtgggg tagaagagga tctccagcgg
     1561 cgtcgtgccc gtggtcagtc cgaagaccgt cttcaaggcg tcgtccgtgt aggcgaaggg
     1621 tacgaagccc cggagcgtct ggagaatgcc cgtcgggtgc tggttgcgga agaagcctga
     1681 taggtagatc agggggaaca gaatcgccgt cgcgtacagg agcacgtcgg ccggcgagga
     1741 ggtgaagttg gcgatgaata ccccgatgca gttcgc
//
LOCUS       DATAIR010000555         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_62986, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000555 DATAIR010000000
VERSION     DATAIR010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62986"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1468)
                     /locus_tag="VHM69_13125"
     CDS             complement(155..1468)
                     /locus_tag="VHM69_13125"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEX2741381.1"
                     /translation="MKIAFIGAGSVVFTKSLMTDTLSFPELHGSTIALHDIDQERLET
                     AGMMARWTSEQLGAGAKVEEHLDRRAALEGADFVVNMVQIGMHQSTLINFEIPKKYGL
                     KQTIADSMGIGGIFRGLRTIPFVLDLAGEMVELCPKALLLNYTNPMSILMEALYTARP
                     EIKSVGLCHSVPYTAREISSYIGVEHEDLLFECAGINHIAWMTRLEVDGEDVYPRLFE
                     AMDDPDVYARDKVRFELMRRFGYFVTESSEHNAEYVPYILKDDDLIERYDVPVDEYIR
                     RSEENLVQYGETRRKLLAGEDFPLDRSVEYGSLIIHSLVTGEARAVYGNVENTGLITN
                     LPEGCCVEVPILVDGTGLRPCHVGDLPPQCAATCAPHTAVQNLTVRAALEGRRDHVHH
                     AAMLDRHAPSVLSLDEIADMVDELIEAHGDAMPEGVRNPRAKARR"
     gene            complement(1518..>2004)
                     /locus_tag="VHM69_13130"
     CDS             complement(1518..>2004)
                     /locus_tag="VHM69_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741382.1"
                     /translation="DMLMWHPEDPVHSAALQLVNVLFSVPQISVLLDRIPPEHIEMLR
                     FWLGFWREHRDVFLDGAIEPRHPEAVYPVVLSRTDTKLAAAAYGNSVVPLEGPLPPTL
                     LVVNGTLENGIVLELAGDAGTREMEVRDCRGRVTCTDSIQLETGLHQIDIPAAGVAVL
                     R"
BASE COUNT          343 a          616 c          638 g          407 t
ORIGIN      
        1 gcctcttccc ggcaccctgc tccctctacc ttctatccct tccccatccc ggtagaggaa
       61 ccgagcttgc ccgaatattg tcacaccaat tttgccctgt caagtatctg gcgtagcacg
      121 tgcaggctct tctgcgccct gacccgaggg gtctctaccg cctcgccttc gccctaggat
      181 tccggacgcc ctcgggcata gcgtcgccgt gggcttctat cagttcgtcc accatgtctg
      241 cgatctcgtc cagggagagg acgctcgggg cgtgcctatc gagcatggcg gcgtggtgga
      301 cgtggtcgcg ccttccctcc agcgccgccc tgacggtgag gttctggacg gccgtgtggg
      361 gggcgcaggt ggccgcgcac tggggtggga gatccccaac gtggcacgga cgcaggccgg
      421 tgccgtcgac caggatcggc acctcgacgc agcatccttc ggggaggttg gtaattagac
      481 cagtgttctc gacgttgccg tagacggcgc gcgcttcgcc ggtgactagc gagtggatga
      541 tgagcgagcc gtactcgacg ctgcggtcga gcgggaagtc ctctccggcg agcagctttc
      601 ggcgggtctc gccgtattga acaaggttct cctcgctgcg cctgatgtac tcgtcgacgg
      661 gcacgtcgta gcgctcgatg aggtcgtcgt ctttgaggat gtaggggacg tactcggcgt
      721 tgtgctcgct cgactcggta acgaagtagc cgaaacgtcg catcagctcg aagcgcacct
      781 tgtccctggc gtacacgtca gggtcatcca tcgcctcgaa gaggcgcggg tatacgtcct
      841 cgccgtctac ttcgaggcgc gtcatccagg cgatgtggtt gatgccagcg cactcgaaga
      901 gcaggtcctc gtgctccacg ccgatgtacg acgagatctc acgcgccgtg taagggacgc
      961 tgtggcagag cccgacgctc tttatctcgg gacgggcggt gtatagcgcc tccatcagga
     1021 tgctcattgg gttcgtgtag ttgaggagca gcgccttcgg gcagagctcg accatctcgc
     1081 ctgccaggtc gagcacgaaa ggtatcgtgc gaaggccacg gaatatgccg ccgatgccca
     1141 tggagtcggc gatcgtctgc ttcaggccgt acttcttcgg gatctcgaag ttgatgaggg
     1201 tcgactggtg catcccgatc tggaccatgt tgactacgaa gtcagcccct tcgagcgcgg
     1261 cacgccggtc gagatgttcc tcgaccttag cacccgcgcc gagctgttcc gaagtccagc
     1321 gggccatcat gcccgctgtc tccagacgct cctggtcgat gtcgtgcaag gcgatcgtgc
     1381 tcccgtgcaa ttcgggaaag ctcagggtgt cagtcatcag gctcttggta aagacgacgc
     1441 tcccggcacc gataaaggct atcttcaaga ctcggtcctt tctctgaatg agcaacctgg
     1501 aatccccacg gtccacatta cctcaataca gccacgccgg cagcggggat gtcgatctgg
     1561 tgcaatcctg tttcgagctg gatgctgtcc gtacacgtca cgcgtcctct gcaatcccgc
     1621 acctccatct cgcgggtgcc tgcgtcgcca gcgagctcga gaacgatacc gttctccagt
     1681 gtgccgttga cgacgagcag cgtcggagga agcgggcctt cgagcgggac gaccgagttc
     1741 ccgtaagcgg cggcggcaag cttcgtatcc gtgcgcgaga gcacgacggg gtagacggcc
     1801 tcggggtggc gcggctcgat agctccgtcc aaaaacacgt ctcggtgttc gcgccagaag
     1861 cccagccaga agcgcagcat ttcgatgtgt tcgggcggta tccggtcgag gaggaccgag
     1921 atctggggca cgctgaagag gacgttcacg agctgaagcg ccgcgctgtg tacgggatct
     1981 tcggggtgcc acatgagcat gtcg
//
LOCUS       DATAIR010000556         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_452686, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000556 DATAIR010000000
VERSION     DATAIR010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452686"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VHM69_13135"
     CDS             complement(<1..597)
                     /locus_tag="VHM69_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2741383.1"
                     /translation="MSERDNSPLWGGRFGTSPAEAFERLNASIPFDMRLAPYDIRGSK
                     AHARMLGAQGIVSEEEAAELIRGLEAVMREVEAGEFSWDLSDEDVHTAVERRLRELVG
                     DVALKLHTGRSRNDQVALDMHLFVRDAAERIRGAVLAAMRSFVEVAENNRDLVLPGYT
                     HLQRAQPILLSHHLLAHFWAFKRDLRRLDAASEAAGVSP"
     gene            694..1116
                     /locus_tag="VHM69_13140"
     CDS             694..1116
                     /locus_tag="VHM69_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741384.1"
                     /translation="MRGWTGSLYWLVAGAVVGFGLIGIMTIGFPFLIIGMVLCVVGVW
                     KPGIGRSWAFLIGLGGLPALVFLRHITDAALTALNPYCSEYFNNPGTGVPRSVGTVTC
                     YYTPASYYVMFVISVAITLSGVVVYLFLRARSGDGAPA"
     gene            complement(1172..1576)
                     /locus_tag="VHM69_13145"
     CDS             complement(1172..1576)
                     /locus_tag="VHM69_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX2741385.1"
                     /translation="MDRKERVARLVDHATNPRHKGTLEDADVAMPGGGPECGGSVVVY
                     LKGDGDGGIEDLSWTGQGDTISTGAASIIMQRVHDEGLGMDEVLDLDYGIFVDGLGRD
                     IIGSRTSNATLGLSTLKNAVRKYQRHLRAGAR"
     gene            complement(1592..>2143)
                     /locus_tag="VHM69_13150"
     CDS             complement(1592..>2143)
                     /locus_tag="VHM69_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2741386.1"
                     /translation="LFGHDNVKVMDGGRVKWEAEGREMTQDVPSFPRAEYPVPTRDDS
                     SIRAFRDDVMAHLEKVSSGEAGLIDVRSPGEYKGELLHMPDYPQEGALRGGHIPGAAN
                     VPWARAANEDGTFKSAEELRSIYEGEAGLKAEGDTIAYCRIGERSSHTWFVLTYLLGY
                     GNVRNYDGSWTEWGNVVRAPIER"
BASE COUNT          367 a          695 c          633 g          448 t
ORIGIN      
        1 cggcgaaacg cccgcggcct cgcttgccgc gtctaacctc ctcagatccc tcttgaaggc
       61 ccagaaatgg gcgagcaggt ggtgggagag caaaatcggc tgggcgcgct ggagatgagt
      121 gtaaccggga agtacgaggt ccctgttgtt ctcggccacc tcgacgaacg aacgcatcgc
      181 ggcgagcacg gcgccacgta tccgttcagc cgcgtccctg acgaagaggt gcatgtcgag
      241 cgcgacctgg tcgttgcggc tgcggcccgt gtgcagcttg agcgcgacgt cgcccacaag
      301 ctccctcagc ctgcgctcga ccgccgtgtg cacgtcctcg tccgagagat cccaggaaaa
      361 ctccccggcc tcgacctccc gcatcacggc ttcgagtcct cttataagct ccgccgcctc
      421 ttcctccgag actattccct gagccccgag catcctggcg tgggctttgg agccgcgaat
      481 atcgtaggga gcgagacgca tatcgaaggg gatggaggcg ttgagacgct cgaaggcttc
      541 ggccggcgag gtgccgaacc ttcctcccca gagaggacta ttgtctcgct cgctcatggg
      601 cggcagttta gccgaacgaa ggcaggccga ccggagggcg tgtgggacag atataatgcc
      661 tgcaacacgt ttcttcttgc gaggagatca ccgatgcgag gctggacggg ttctctctac
      721 tggttggtgg ctggcgccgt tgtgggtttc gggttgatcg gcatcatgac catcgggttc
      781 ccgttcctga tcatcggcat ggttttatgc gtggtcggcg tctggaaacc gggaatcggc
      841 agaagttggg cgttcctgat cgggctcggt gggctacctg cgctcgtctt tctccgtcac
      901 attaccgacg ccgcgttgac cgcgttgaac ccgtactgct cggaatattt caataaccca
      961 gggacaggcg ttccacgcag tgtgggcacg gtgacgtgct attacacacc cgccagctac
     1021 tacgtgatgt tcgtgatctc ggtcgcgatc accctctccg gagtagtcgt gtatctgttt
     1081 ttgcgtgccc gttcgggcga tggcgcccct gcctgaaaaa cccgtcagga gagtctcacg
     1141 aagctctagt gcgaccgcaa cgcgcgggca gctagcgggc acccgcgcgc aagtgcctct
     1201 ggtacttcct cacggcgttc ttgagagtgc taaggccaag cgtcgcattg ctggtgcggc
     1261 tgccgatgat gtcgcgaccg agcccatcga cgaatatacc gtagtcgaga tcgaggacct
     1321 cgtccatccc gaggccctcg tcgtgcaccc tctgcatgat gatagatgcc gcacccgtgc
     1381 tgatagtatc gccctgtccg gtccacgaga ggtcctctat cccgccgtcg ccatcacctt
     1441 tgaggtagac gacgacggac cctccacact cgggaccgcc accgggcatt gccacgtctg
     1501 cgtcttcaag tgtccccttg tggcgcgggt tcgtggcatg atccactagc cgcgccacgc
     1561 gctctttgcg atccaaaagt ctcctggcgc tctatctctc tatgggagca cgcacgacgt
     1621 tgccccactc cgtccaacta ccgtcgtagt tccttacatt cccgtagcca agcaggtaag
     1681 tgagcacgaa ccacgtgtgg ctcgagcgct ccccgatgcg gcagtaggcg atggtgtccc
     1741 cttcggcctt gaggccagcc tctccttcgt agatgctccg cagttcctct gcgctcttga
     1801 aggtcccgtc ttcgttggcc gcccgagccc acggaacgtt ggccgctccg gggatgtgac
     1861 caccgcgcaa tgcgccttcc tgcgggtagt caggcatgtg gagcagctct cccttgtact
     1921 ccccagggct cctcacgtcg atcaggccgg cttcaccaga agagaccttc tccaggtgcg
     1981 ccatcacatc atcgcgaaac gcccttatac tcgagtcatc gcgggtcggg acagggtact
     2041 cagccctggg gaaggaaggc acatcctggg tcatctcgcg accctccgcc tcccacttga
     2101 cccttcctcc gtccatgacc ttcacgttgt cgtggccgaa gag
//
LOCUS       DATAIR010000557        13761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_130428, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000557 DATAIR010000000
VERSION     DATAIR010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130428"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VHM69_13155"
     CDS             complement(<1..371)
                     /locus_tag="VHM69_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX2741387.1"
                     /translation="MFEGLQAQGEINRIIARDEVLDDEFIARQRARLEDIRRTLLRER
                     AGIQEDERQWDEDGHYAPRDVEDVRAHILAEDLDAVLDRQLIKRLRSVERALEKIEEG
                     TYGICDATGEQIPKGRLEAVP"
     gene            complement(405..731)
                     /locus_tag="VHM69_13160"
     CDS             complement(405..731)
                     /locus_tag="VHM69_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741388.1"
                     /translation="MTALGGVKVRIEGHYEVNDLSYGRDYVWVPAHALVECGCGQVMD
                     ADAHHTTCPNCGADHSDVMREVVGRHLSDEVLHPWRPDYEEWRRFNENRTEYQEWLEL
                     QSLDKD"
     gene            complement(823..1269)
                     /locus_tag="VHM69_13165"
     CDS             complement(823..1269)
                     /locus_tag="VHM69_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2741389.1"
                     /translation="MNGLPTKILVATDGSKDAAMAARRAAEMAHAFGSELHMVHVVPV
                     AQPYHLFGADAEGPILYEEDKQWAQEMLDGQVRLVEESGGKVARTHLREGEPDAEVVS
                     LGEEIGANMIVTGSRGLGRMRRPIGSISSSIAAHAHCPVLVVRGSA"
     gene            complement(1307..3718)
                     /gene="ppsA"
                     /locus_tag="VHM69_13170"
     CDS             complement(1307..3718)
                     /gene="ppsA"
                     /locus_tag="VHM69_13170"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX2741390.1"
                     /translation="MLKTRWTRWFSEIGIDDVPLVGGKNASLGEMIRELGSLGVRVPE
                     GYSVTADAYRYFIEANELEAAIKEAMAGLHGYDVEGLTRGSHMIQNLILGGYIPEDLE
                     TEILRGYRELSDRYGEEHADVAVRSSATAEDLPTASFAGQQESFLNVRGEAQLLESVK
                     RCFASLFTPRAISYREDMGFDHFQVALSVGVQKMVRSDLASSGVIFTLDTESGFPDVV
                     FVTSTWGLGENIVQGRVVPDGFYVHKPTLELGYTPLIRKRLGTKEFKLIYDARGHKLV
                     ENVPTSEEERETFSVSDENVLQLARWALKIEGHYSDKRGTRTFMDVEWARDGLTGELF
                     ILQARPETVHGGHAAPTLRMHRLKEQGRVLVEGLAIGNAVATGPVRVLKDPSEIESFR
                     SGEVLVTEITDPDWEPIMKISSAIVTERGGRTSHAAIVARELGIAAVVGTENATEAIK
                     TGGPVTVSCCEGEVGRVYEGVLDYEVTELDPATVGRPKTQIMMNVADPERVFELSQIP
                     NDGVGLARMEFVFAGWVGIHPLALTRYSSLPPKVRLEVSRLTRGYEDKTEFFVERLAQ
                     GIGTLAAAFWPRPVILRFSDFKTNEYAHLLGGDLFEPHEENPMLGWRGASRYYHPDYK
                     EGFLLELQAVKRVREVFGLKNLKVMIPFCRTPEEGRRVLEVLEEGGLVRGEDGLEIYV
                     MAEIPSNVILAKEFAEIFDGFSIGSNDLTQLILGVDRDSERVAPLFDERDQAVKLMCA
                     TLIEEAHRAGRKVGICGQAPSDYPDFAAFLVERGIDSVSLSPDAVVPTAMKILEVEQR
                     TDRTG"
     gene            3952..4326
                     /locus_tag="VHM69_13175"
     CDS             3952..4326
                     /locus_tag="VHM69_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741391.1"
                     /translation="MVNTAQHVPHPPLWRRLLSFPKTRLGWWSVGFAATSVVMLIFGY
                     GVFEVLMVRVLGRYQETQWEGMAITFYGITMLLCGLAGGVVSLIASIRQHERSWLVWL
                     SILTLLVDLFLVIGEFLIPPFD"
     gene            complement(4419..4889)
                     /locus_tag="VHM69_13180"
     CDS             complement(4419..4889)
                     /locus_tag="VHM69_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741392.1"
                     /translation="MLKVLYALAIALLVVAFVGFGISAFYPAPEYPELPTELEYVGKN
                     QTDRQTQLIAEQRQKEEAYQKEFSDYNQVVSSISIGIAVLLLVGSILWLSSLDVIGDG
                     VTLGAVFTLFYGLIRAFMTESEVFRFIAVGVGLVVVITLVYLRFVRSAGKAEPA"
     gene            complement(5114..5326)
                     /locus_tag="VHM69_13185"
     CDS             complement(5114..5326)
                     /locus_tag="VHM69_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741393.1"
                     /translation="MDSDKQKEERSTELASPREHVQEMEGHLQEMGEYLRRMGERLRK
                     LDAQLSHLEAEDDRAVDRGRGWPTGG"
     gene            complement(5407..5895)
                     /locus_tag="VHM69_13190"
     CDS             complement(5407..5895)
                     /locus_tag="VHM69_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741394.1"
                     /translation="MHSISLHQKGAGSLAGGTASYPTPWYWLLTRVRVSGLEVFTENT
                     SKGEEILPVFGSADDAIGYLTEEPGSWKPRKTGRGELASLLMGVCREVRWVTLDPPPR
                     VATDEVLKVLGLSRDTFLEPLLGRDRSWFEEERNRRQRSSAYTLLSRTIRMTTVEARK
                     AF"
     gene            complement(6270..7457)
                     /locus_tag="VHM69_13195"
     CDS             complement(6270..7457)
                     /locus_tag="VHM69_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2741395.1"
                     /translation="MLNGKGDAYQEQIVSLRRHLHRHPELSGEEHATSEMVQEKLREH
                     DIPFTAGYAGTGVLGVVEGGRPGGTVALRADMDALPIEEKNDHSFISKVDGVMHACGH
                     DAHTAMLVGAGWLLNDVRDELPGRVLLLFQPAEENSPTGGARQMIKDGVFREHRPDAM
                     FAQHVSPELPVGQVGVRRDAMTGASDRFRVTIQGEGGHASKPHQTTDAIVVANQIINS
                     LQTVVSRDVDPLRSAVLTIGKIRGGTRYNAIAEKATLEGTIRTFGPQTKETVKDRFHA
                     IVRGVAEAMEAEAQIRYWDGYPATVNTPEWAERVRKTAQDLLGTDATPEIDPSLGGED
                     FGRFLLEYPGAYFRLGTASSNSTERQRLHDSRFDIDERALHIGTELIARLAVDALCQL
                     NDR"
     gene            7657..8376
                     /locus_tag="VHM69_13200"
     CDS             7657..8376
                     /locus_tag="VHM69_13200"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741396.1"
                     /translation="MYGKPVALWAVPRSVSTALERVFVERGDFKVFHEPFSATYYYSP
                     ERRSDRFAGTAEEYGHEKLLARMLEYREKPVYFKDMAYHVAGFMTPEFVSRFTNTFII
                     RDPAPVIASLYRFWPDFTLEETGYEQQHRLFGLAVENGEDPAVVDAADLTADPEGTIR
                     AYCEKLGVRFMPESLSWEPGEVPGWEMWKEWHEEAQESTGIKGEPLEDDTEVPAGLES
                     IYERCLPYYEELYEKRLQSAR"
     gene            8437..9093
                     /locus_tag="VHM69_13205"
     CDS             8437..9093
                     /locus_tag="VHM69_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2741397.1"
                     /translation="MALALYDLDNTLLAGDSEHAWCEFLVDAGVLDGDEARAENERLY
                     GEYLAGNLDIHESVKFQAAPLAKHPTDDLHRWRREFMASRVETMITESAVALVEEHRE
                     AGDDLAIITASNSFVTEPIADRFGVPTLLAVELERHERRYTGRILGTPTFREGKITRL
                     TEWMEDSGHSLDGSYFYSDSHNDLPLLKLVDNPVAVNPDPTLSAHAEAEEWPVLRLHG
                     "
     gene            9189..10184
                     /locus_tag="VHM69_13210"
     CDS             9189..10184
                     /locus_tag="VHM69_13210"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEX2741398.1"
                     /translation="MDSEAQEAVGETRPVTVAPSGAALKRIQDVLAQVPLFGDTRWED
                     FEIEALNSFTNLSYKLSAQSDAYVLRVAGKGTSTYIDRSAEEHNARIATEAGLNAEVL
                     YFDAKDGTMLSRFIEGSHMDKMEFHRDPTAPSRAALTLKRLHGIGRTFKSRFGPYAPI
                     DYYLQLLRTLRSSLPDVYEEIKGEAEAVRRMLDATSVLTTPCHNDTCPENFVEVGRRI
                     YLIDWEYSGMNDPMWDLGNLSVEAAFGYEQDCKMMEAYCEEAVLPGLYERMVLQKAMS
                     DYFWGLWSIVQHANGNPAADFWTYAIDRFERCKALMSSDGFGLHLDAVSAGYRAK"
     regulatory      10390..10475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10518..11816
                     /locus_tag="VHM69_13215"
     CDS             10518..11816
                     /locus_tag="VHM69_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2741399.1"
                     /translation="MTSRHISRKEFLGIGAGAAAGVALAGSGMATFLSKEAGALTSFK
                     PFTVSLPIPPKAVEDADGVIRLFAQQSTQAMHPRLPKVKVWGYNQVDPDPTLPPEYNK
                     TSGYLGPTIEVDKNGPNPTNVEFTNNLPPTHLLPVDDNLPRPDGGTERGTSPRILTHL
                     HGSFVDGDNDGNPYATQNEYETGSVAGATQSVTYPSQPRATMLWYHDHAMAMTRLNVY
                     AGLAGFFLVRDNNDTGGEDNPIGIPGGPYEVPILIQDKSFTSGGQLFYSATAEWIPEF
                     FGNTPVVNGAVRPFLTVEPRMYRLRFLNGSQSRFYNLEMAIGDANAALLPDRLRGRHV
                     RRTGANQPDPAFACRASRRDSGFQGPRRPEYRAQEPLAAQGSDQPRYAEDTEPDAVPG
                     PWDGQHARADVDPDPAARLQAEPSRLRRRQDPLHHPRRGP"
     gene            11833..12342
                     /locus_tag="VHM69_13220"
     CDS             11833..12342
                     /locus_tag="VHM69_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2741400.1"
                     /translation="MRLAIDGRFFDDGADEDDDAVDERPLAGTVEDWSFVNVSADTHP
                     IHMHLTHFQVMGRTPLNGVPYAQDLENARAIAANQPLPYPTRPAAPPLLANNTIDPTP
                     YLLGPEVSPAPDEQGWKDTVGAHPNQVTRIRQRFDLPADAPVPQKYVYHCHILEHEDN
                     DMMRPYDVV"
     gene            12733..13170
                     /locus_tag="VHM69_13225"
     CDS             12733..13170
                     /locus_tag="VHM69_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2741401.1"
                     /translation="MLGPFRRFWDMQSEMNRMMSDAIGRAPQASITGAGWTPSVDVVA
                     REGDLVIKAELPGMKREDVEVSFQNGVLTISGERREEEEHKDAGYLVKERRYGSFSRS
                     MTLPEGIDEQKIKASFNDGLLEVMVQGAGAQVAEGPRRIEIGD"
     gene            complement(13210..13443)
                     /locus_tag="VHM69_13230"
     CDS             complement(13210..13443)
                     /locus_tag="VHM69_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2741402.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDQFLINPEECIDCG
                     ACEPECPVEAIYPEDEVPDDMEAFITKAEDYFE"
BASE COUNT         2686 a         4342 c         3978 g         2755 t
ORIGIN      
        1 tcaggcacgg cctcgagcct gcccttcggg atctgctccc ccgtggcgtc acagatgccg
       61 tatgtccctt cttctatctt ctcaagcgcc ctttccacgg accggagccg ctttatgagc
      121 tgccggtcga ggacggcatc aaggtcttcc gcgagaatgt gggccctgac gtcttcaacg
      181 tcccttggcg cgtaatgtcc atcctcgtcc cactgcctct catcctcctg gatgcctgcg
      241 cgttctcgca gcagcgtgcg cctgatgtcc tccaggcggg cccgctggcg ggctatgaac
      301 tcatcgtcga gcacttcgtc ccgcgctatg atgcggttga tctctccctg cgcttgcaga
      361 ccttcgaaca tgtcggacac agcctttccg tttcgtggac gttctcagtc cttgtcgaga
      421 gactgcagct cgagccactc ctggtactcg gtccggtttt cgttgaacct gcgccactcc
      481 tcgtaatccg gacgccaggg atggaggacc tcgtcgctga ggtgtctgcc taccacttcc
      541 ctcatgacat ccgagtggtc ggcgccgcaa ttcggacacg tcgtgtggtg tgcgtcggcg
      601 tccataacct gaccacatcc gcactcgacg agcgcgtgag cggggaccca cacgtagtcc
      661 ctgccgtagg agaggtcgtt cacctcgtaa tgtccctcga tacgtacctt gaccccgccc
      721 aatgcggtca cctccttgct cgatcttctc ctacccaaac gtaccgcgat gtaataacag
      781 cggcctgatc aatttggccc atttatctgg caagtattgt cctcaagcgc ttcccctcac
      841 cacgagcacg gggcagtggg catgcgcagc tatgctgctc gagatgcttc cgatgggccg
      901 tctcatccga ccgagccccc tgctcccagt gacgatcatg ttcgccccta tctcctctcc
      961 gagcgagacc acctccgcgt ccggctctcc ttccctcagg tgcgtcctgg cgaccttccc
     1021 gccggactcc tcgaccagcc tcacctgtcc gtcgagcatc tcctgagccc attgcttgtc
     1081 ctcttcgtaa aggatgggcc cctccgcgtc tgctccgaac aggtggtacg gttgcgccac
     1141 gggtacaacg tggaccatgt gcagctccga gccgaacgcg tgggccatct ccgccgccct
     1201 tcgtgccgcc atggcagcat ccttcgatcc gtcggtggcg acaaggatct tcgtcggtag
     1261 accgttcatc tcttcccttt cgtagcgttc ctcaacgtca tgctcgtcaa cctgtcctgt
     1321 ccgttctctg ctccacctcc aggatcttca tggccgtcgg caccaccgcg tcggggctca
     1381 ggcttactga atcgatccct cgctcgacca ggaaggcggc gaaatcgggg tagtcagagg
     1441 gggcctgacc gcagatcccg accttgcgtc ccgccctgtg cgcctcctct atgagcgtgg
     1501 cgcacatgag cttcacagcc tggtcacgct cgtcgaagag cggtgccacc cgctccgagt
     1561 ccctgtccac cccgaggatg agctgggtga ggtcgttgga gccgatagag aatccgtcga
     1621 agatctcggc gaactccttc gcgaggatga cgttggaggg tatctcggcc atcacgtaga
     1681 tttcgagccc gtcctcgccc cgcacgagcc cgccttcttc cagaacctcg agaacccttc
     1741 gcccctcctc cggggtgcgg cagaacggga tcatgacctt caggttcttg agaccgaaga
     1801 cttcgcgcac ccgcttgacc gcctggagtt cgagcaggaa gccctctttg tagtcggggt
     1861 ggtaatagcg gctcgccccg cgccagccga gcatcgggtt ctcctcgtgg ggctcgaaga
     1921 ggtcgccgcc gaggaggtgg gcgtactcgt tggtcttgaa gtccgagaag cggagtatga
     1981 cgggtctcgg ccagaacgcg gcggccagtg tacctatccc ctgagcgagc ctttcgacga
     2041 agaactccgt cttatcctca tagcctcggg taaggcgcga gacttcgagc ctgaccttgg
     2101 gcgggagcga ggagtagcga gtgagggcca gcggatgtat cccgacccaa ccggcgaaga
     2161 cgaactccat acgagcgaga ccaacgccgt cgttcgggat ctgggagagc tcgaagaccc
     2221 gctccgggtc tgcgacgttc atcatgatct gggtcttcgg acggcccacc gtcgcgggat
     2281 ccagctcggt gacctcgtag tcgaggacac cttcgtagac gcgccccacc tcaccctcgc
     2341 aacaagagac ggtgacgggg cctccggtct tgatggcctc ggtcgcgttt tccgtaccta
     2401 ctacggccgc aatcccaagc tcgcgggcca cgatcgccgc gtgcgaggtg cgtcctccgc
     2461 gctcggtcac tatggctgag gagatcttca tgatcggctc ccagtcgggg tcggtgatct
     2521 ccgtgacaag cacctcgcca cttcggaagc tctctatctc gctcgggtcc ttgagcaccc
     2581 ggacagggcc ggtcgcgacc gcgttaccga tagcgagacc ctcgacgagc acccttcctt
     2641 gctccttgag gcggtgcata cgaagcgtcg gcgcagcatg ccctccgtgc accgtctcgg
     2701 ggcgggcttg caggataaag agttcgcctg tgaggccgtc cctggcccac tcgacgtcca
     2761 tgaacgttct cgttccacgc ttatcgctgt agtgaccctc gatcttcaag gcccagcgcg
     2821 cgagctggag cacgttctcg tcagagacgg agaacgtctc acgctcctct tccgaagtag
     2881 gaacgttctc gaccagcttg tgccccctgg cgtcgtagat tagcttgaac tccttcgtgc
     2941 cgagccgctt ccggatcagc ggggtgtagc ccagttcgag cgtcggcttg tgcacgtaga
     3001 acccgtcggg gacgacgcgc ccctggacta tgttctcgcc aagcccccac gtcgaggtca
     3061 cgaacacgac gtcagggaag cccgactcgg tgtcgagcgt gaagatcaca cctgaagagg
     3121 ccaggtccga gcggaccatc ttctggacgc cgaccgagag tgctacctga aagtggtcga
     3181 agcccatgtc ctcgcggtag cttatggcac gaggggtaaa gaggctcgca aagcaccgct
     3241 ttaccgactc gagcaactgt gcctcgcccc taacgttgag gaagctctcc tgctggcctg
     3301 cgaagctcgc ggtaggcagg tcctccgctg tagcagaaga gcggacggca acgtccgcgt
     3361 gctcttcccc gtagcgatcg gagagctcgc ggtagccacg caggatttcc gtctccaggt
     3421 cttcagggat gtagccgcca aggatcaggt tctggatcat gtggcttccg cgggtgagac
     3481 cttcgacatc gtagccgtgg agccccgcca tggcttcctt gatggcggcc tcgagttcgt
     3541 tggcctcgat aaagtacctg taggcgtcgg cggtcaccga gtacccctcc ggcaccctga
     3601 caccaagcga tccgagttcg cggatcatct ctcctagaga agcgttcttc ccaccaacga
     3661 gcggcacgtc atctatcccg atctccgaga accaccttgt ccagcgtgtc ttcaacatcc
     3721 ctgcaacacc tccgtcagtc accgcatcag cctctatccg tggcggggct ccgtcgttca
     3781 cacccgaact gtacgtgagc tagatcagcg tagcctgagc agaatttccc atatacatgg
     3841 gcagtattga ctattgcggt gcaggcggtc cgctcgtcaa accgcatgga tcccgcttcc
     3901 agagtaatcc ttccctctgt ttagagcccg cgcttccgag acaaggatgc cttggtcaat
     3961 actgctcaac acgttcccca ccctccgctg tggagaaggc tcctctcgtt tccaaagact
     4021 cgcctcgggt ggtggtcggt gggatttgca gccacgtccg tggtcatgct catcttcggc
     4081 tacggcgtct ttgaggtctt gatggtacga gttctaggca ggtatcaaga aacgcagtgg
     4141 gagggaatgg caataacctt ctacggcatt acgatgctat tgtgcggtct ggctggagga
     4201 gttgtctctc tgatcgcgtc gatacgacag catgagcgat cgtggctcgt atggctttct
     4261 attctgacct tgctggtcga cctcttcctc gtgatcggcg agtttctgat tccacccttc
     4321 gactagaatc aagccactgg ccacagcatg tgaaagagtg gctccttaag gagccactct
     4381 ttcacgaggt tgggcgtgga accaggctct cggtaacctc aggcaggttc agccttccca
     4441 gcagaccgca caaacctcaa gtaaacaagc gtgatcacca ccacaagtcc tacccctaca
     4501 gcgatgaacc tgaacacctc gctctccgtc atgaatgccc tgatcaaacc gtagaagagg
     4561 gtgaacactg ccccaagggt cacaccatcg cctatgacat ccaggctgct tagccacagg
     4621 atgctgccaa cgaggagcag caccgcaatc cctatggaga tggatgagac tacctggttg
     4681 tagtctgaga actccttctg gtatgcctct tccttctgcc tctgctcggc gattagttgt
     4741 gtctgcctat cagtctggtt cttacccacg tactctagtt cggtaggaag ctctgggtat
     4801 tcaggagcag ggtagaaggc agagatcccg aagccgacga aggcgacgac gaggagggct
     4861 atggccagag catagagtac cttgagcatc ttgagccgtc ctcctcgaag tcatccaaac
     4921 tatgttagcc cctgagcagg tacccgtctt gacgaatctc gaccaggtaa gtggtcagat
     4981 tgtgtcattc gcctcgcgaa gtttcatgag aaggcacatt ctggtcgagg ctggtaccgg
     5041 gccctgggcc ggtgagatgt tcccaccgca gcgagagagt gcaggctcat gttcgacggg
     5101 cccgactacg aagctatcct cctgtgggcc agcccctccc acggtctacg gcacggtcgt
     5161 cctctgcctc caggtgactt agctgcgcgt ccagcttacg cagccgctcg cccatacgcc
     5221 gcaggtactc ccccatctct tgcaggtgtc cctccatctc ttgcacgtgc tctctcgggc
     5281 ttgcaagttc ggtgctccgt tcttcttttt gcttatcgct atccattggc tccttccctt
     5341 cttcctctcc gtaaacactg atagcaagcc catgcggcat ctccagcgcc gcacgacaat
     5401 gtaaaactag aatgccttcc tggcttcgac tgtagtcatc ctgatagtgc gggacagtag
     5461 agtgtaggcg gacgacctct gcctcctatt gcgctcctct tcaaaccagg agcggtccct
     5521 tccgagaagc ggctccagga atgtgtcccg agacagaccg agtaccttca ggacctcatc
     5581 cgtcgccacc cgcggcggag gatccagcgt cacccacctg acttctctgc acactcccat
     5641 caggagggag gcgagttctc cgcggccagt cttcctcggc ttccaggaac caggctcttc
     5701 ggtgaggtag ccaatggcat catccgcgga gccgaagacg ggcaggatct cctcaccctt
     5761 cgacgtattt tcagtaaaga cctccagccc gctgaccctc actctggtca agagccagta
     5821 ccacggcgtc gggtatgagg cggtgcctcc cgcgaggctt cccgctcctt tctgatgcaa
     5881 cgaaatgcta tgcattcctg ctcctccata agccatcggg ggttacctgg tggactccac
     5941 gctatccgtg gccagtggat aacggtagtc ctggtccttg ctcacactgt aagacgcgta
     6001 tccggcatgc gcgaacggcg cgacggagtt tcacgctgag gtttcccctg cctacgagtc
     6061 cctggtcctg cctggccgcc cgcacaacga cctctctccg ccttcttgcg tatcccttgc
     6121 gctaattgta tcaacctggg gcgccactgt cattaatgag attcgctttt gcgcatccgc
     6181 caggctcgaa ggcatctaac cttgcggccg ggcgctcgtt ccttatggga tccgctacag
     6241 agtagacgaa aagttggacc acaatcactc tagcggtcat tcagttggca aagggcgtca
     6301 acggctagcc gggcgatgag ttctgttcct atgtggagcg ccctctcgtc aatgtcgaaa
     6361 cgcgagtcgt gaagccgctg cctctctgtg gaattcgaag acgccgtccc gagcctgaag
     6421 taggcaccgg gatactcgag caagaagcgt ccgaaatcct cgcctcccag ggaaggatct
     6481 atctcgggcg tcgcgtcggt cccgagcagg tcctgcgccg tcttacgtac acgctcggcc
     6541 cactccgggg tgttgacggt tgccgggtag ccgtcccaat acctgatttg ggcctcagcc
     6601 tccatcgcct cggcgacgcc gcgtactatc gcatggaagc ggtctttgac cgtctccttg
     6661 gtctgcggcc cgaacgtgcg gatcgtgcct tcaagcgtcg ccttctcggc tatggcgttg
     6721 tagcgggtcc cgccccttat cttcccaatg gtaagcaccg cggacctgag agggtcgacg
     6781 tccctactaa cgaccgtctg caggctgttg atgatctggt ttgccaccac tatggcgtcg
     6841 gtcgtctgat ggggtttgct cgcgtgtccg ccctcaccct ggatcgtcac cctgaagcgg
     6901 tctgaggccc ccgtcatcgc gtcccggcgt acgcccacct gtccaacggg caactcgggc
     6961 gatacgtgct gcgcgaacat ggcgtcgggg cggtgctccc tgaagacgcc atcctttatc
     7021 atctgtcgcg ccccgccggt gggcgaattc tcctccgcgg gctggaacag gagcagcact
     7081 cgtccgggga gttcatccct gacgtcgttc agcaaccatc ccgcccctac cagcatcgcc
     7141 gtatgggcat cgtgtccgca ggcatgcatc accccatcca ccttcgagat gaaggagtga
     7201 tcgttcttct cctctatggg cagggcgtcc atgtcggccc gcagtgcgac ggtcccgccc
     7261 ggcctgcccc cttccaccac cccaagcacg cccgtcccgg cataacccgc ggtaaatgga
     7321 atgtcgtgct cacgcagctt ctcctgcacc atctcagaag ttgcgtgttc ctccccgctc
     7381 agctcgggat gccggtggag atggcggcgc aaggaaacga tctgttcctg gtatgcgtcg
     7441 cccttgccgt tcaacattga tctcaagctt gccatcccgc cgcaggagta ccggccaccg
     7501 ccaattgtat ctccgggacc agttatatgc gcacccttga gaaaatccct tagccagcgc
     7561 cgaaagggat gctatgctcc aggagcgcac aacacatcca gctggacctc caaaaaatcg
     7621 tccgggcggg gcgagaaagg tgaaaggagc gcgttgatgt acggtaagcc cgtagccctc
     7681 tgggcagtgc cccggtccgt ttcgaccgcc ctggagcggg tattcgtcga gcgaggggac
     7741 ttcaaggtct ttcacgaacc gttctccgcc acctactact actcgcctga gcgccgcagc
     7801 gaccgcttcg cagggacggc agaggagtat ggacacgaga agttactggc acggatgctc
     7861 gagtacaggg agaagccggt atatttcaag gacatggctt accacgtggc cggcttcatg
     7921 accccagagt tcgtctccag gttcaccaac acgttcatta tccgggatcc tgctccggta
     7981 atcgcctcgc tctacaggtt ctggcccgac ttcaccctgg aagagacggg ctacgaacaa
     8041 cagcacaggc tgttcgggct agcggtggag aatggggagg accctgccgt agtcgacgcc
     8101 gcagacctga cagcagaccc cgaaggcacg atacgagctt actgcgagaa gctcggcgta
     8161 cgattcatgc cagagtcact atcctgggag ccaggggagg tgccaggctg ggagatgtgg
     8221 aaggagtggc acgaggaggc gcaagaaagc accggtatca agggcgagcc gctcgaagac
     8281 gacacggagg tacctgcggg cctggaaagt atctacgaac gctgccttcc ctactatgag
     8341 gagctgtacg aaaagcggct gcagtccgcc agatagccga aggacgattg cggctatccg
     8401 gtccatccgc ccatgggttg ctatagttac ctgatcgtgg cgcttgctct gtacgatctg
     8461 gacaataccc tgctggctgg tgactccgag cacgcctggt gcgagtttct ggtcgacgcc
     8521 ggcgtgctcg acggggacga agcgcgcgcc gagaacgagc gtctgtacgg cgaatacctg
     8581 gccgggaacc tggacatcca cgagtccgtg aagttccagg cggcacccct cgcgaagcac
     8641 ccgacggacg acctgcaccg gtggcgaagg gagttcatgg cctcgcgcgt cgagaccatg
     8701 atcacggagt cggcggtcgc gctggtcgag gaacaccgcg aggctggcga cgatctggcc
     8761 atcatcacag cgagcaacag cttcgtcaca gagcctatcg ctgaccgttt cggcgtaccc
     8821 acgctccttg ccgtggagct ggaacgtcac gagcgtcgtt acacaggacg catcctcggc
     8881 acacccacct tcagggaagg gaaaataacg cggctcacgg agtggatgga ggatagtggc
     8941 cacagccttg acggcagcta cttctacagc gattctcaca acgacctgcc tttgctcaag
     9001 ctggtggaca acccggtcgc cgtaaacccg gaccccacct tgagcgcgca tgctgaggcc
     9061 gaagagtggc ctgttctccg ccttcatggc tagcctgatc tcgtctggag cccgcggggt
     9121 cgtcgacagg ccgggcatcc gtcgtaggac gatactcgag taacgcgaac ttcacgcgga
     9181 ggagacatat ggatagcgaa gcacaagagg ccgtcggcga gacgcgccct gtgaccgtgg
     9241 cgccatctgg ggcggcgctc aagaggatcc aggacgttct ggcgcaggta cctctgttcg
     9301 gggacacccg gtgggaagac ttcgagatcg aagccctcaa ctccttcacc aacctgagct
     9361 acaagctgag cgcgcaaagt gacgcatacg tgcttcgggt agcggggaag ggcacatcga
     9421 cctacatcga ccgtagtgcc gaggagcaca acgcccgtat cgcgactgag gccggactca
     9481 acgcggaggt gctctacttc gacgcgaaag acggcaccat gctctcacgg ttcatcgagg
     9541 gttctcacat ggataaaatg gagttccacc gagatccgac ggcgccatcc cgcgcagcgc
     9601 tcacgctcaa gcggctacac ggcatcggtc ggaccttcaa gtctcgcttc ggcccatatg
     9661 caccgatcga ctactatctt caacttcttc gcacgctacg ctcctcgctg cctgacgtct
     9721 acgaagagat aaagggagag gcagaggcgg tgcgccggat gctcgatgcg acctccgtac
     9781 tcacgactcc ctgccacaac gacacttgcc ctgagaactt cgtggaggtg ggtaggcgta
     9841 tctacctcat cgactgggag tactcgggaa tgaacgaccc tatgtgggac ctcggcaacc
     9901 tctcggtaga ggcagccttc ggctatgaac aggactgtaa gatgatggaa gcttattgtg
     9961 aggaggccgt gctgccgggt ctctacgagc gcatggtgct gcagaaggcg atgagcgatt
    10021 atttctgggg tctgtggagc atcgttcagc atgctaacgg caatccggcg gcagatttct
    10081 ggacctatgc catagatagg ttcgaacgct gcaaggcgtt gatgagcagc gacgggttcg
    10141 gtctacatct cgatgccgtg agcgcgggct acagagcaaa atagtgacgt acacctggca
    10201 cccactatca cggagtagtc ggcaccagga gagttcccca acaacctggg tgttctcaga
    10261 agttcaagcc ttacaagttg cttggcagtc tcgaaccagt gcgagaagtg gccgttggca
    10321 aaggtggatt ttgaccaatg aattggccca ttttcatcaa gagtagcgct gttcagtacg
    10381 ctatcttgcc tggcaagtag aggtcaagaa gcctatccca ctattgtggg cacctgggat
    10441 gggaagagcc agtggtgcaa ccggctactc gtaaagagag tggccggttt tgttgttttt
    10501 gggaaaggag acacgctgtg acaagcagac atatatctag gaaggagttc ctcgggatcg
    10561 gtgcgggggc agccgccggt gtcgcgcttg ccggatcggg gatggcgacg ttcttgtcga
    10621 aggaagccgg cgctctcacc tcgttcaagc cgttcacggt gtcgttgcca attccgccca
    10681 aggcggtcga ggacgctgac ggggtgatcc ggctctttgc ccagcagtcc acccaagcga
    10741 tgcatccgcg gctgccgaaa gtgaaggtct ggggctacaa tcaggtagat ccagatccca
    10801 ccctgccccc cgagtacaac aaaacgagtg gctatctcgg cccgacaatc gaggtcgaca
    10861 agaacggtcc gaacccgacc aacgtagagt tcaccaacaa cctgcccccc acgcatctgc
    10921 tgccggtcga tgacaacctg ccccgcccgg acggaggaac ggagcgagga accagccccc
    10981 gcattctgac gcacttgcac ggcagtttcg tcgacggcga caacgacggc aacccctacg
    11041 ccactcagaa tgagtacgaa acaggaagcg tcgcaggcgc gacgcagagt gtgacctacc
    11101 cgagccagcc gcgcgcgacg atgctgtggt accacgacca tgcgatggcg atgacgcgcc
    11161 tgaacgtcta cgccgggctt gctggcttct tcctggttcg cgacaacaac gacaccggtg
    11221 gagaggacaa cccgattggg atcccaggcg ggccctatga ggtaccgatc ttgatccagg
    11281 acaagtcgtt tacgtccggc gggcagctct tctacagcgc cacggctgag tggatccccg
    11341 agttcttcgg caacacgccc gttgtaaacg gtgcggtgcg gccgttcctg accgtcgagc
    11401 ccaggatgta ccgcttgcgg ttcctcaacg gctctcagtc gcgcttctac aacctcgaga
    11461 tggccatcgg agacgctaac gccgcccttc taccagatag gctccgaggg cggcatgttc
    11521 gacgaaccgg tgcaaaccag ccggatcctg cttttgcctg ccgagcgagc cgacgtgata
    11581 gtggatttca ggggcctcgc cggccagaat atcgagctca agaaccgctc gctgcccaag
    11641 ggagtgatca gccccgctac gccgaggata ctgagcctga tgcagttccg ggtccgtggg
    11701 acggccagca cgccagggcc gacgtcgatc ccgaccccgc tgcccggctc caagccgaac
    11761 cttcccgact ccgacgtcgt caagacccgc tacatcaccc tcgaagaggt ccttgacgag
    11821 gatggcgacc cggtgcggct ggctatcgac gggcgcttct tcgacgacgg ggcggacgag
    11881 gatgacgacg cagtggacga gaggcccttg gcgggcacgg tagaggattg gtcgttcgtc
    11941 aacgtaagcg cggacacaca cccgatccac atgcacctga cccacttcca ggtgatggga
    12001 cgcacaccgc tgaacggagt cccgtacgcg caggacctcg agaacgcccg ggccatagcc
    12061 gcgaaccagc cgctgcctta cccgacgcgg ccggcggcgc cgccgctgct cgcgaacaac
    12121 accatcgacc ccacgccgta cctgttgggg ccggaggttt cgccagcacc tgacgagcag
    12181 ggctggaagg acactgtcgg cgcacatccc aaccaggtca cacgcatccg gcaaaggttc
    12241 gaccttcccg cggacgcccc ggtgccgcag aagtacgtct accactgcca catcctcgaa
    12301 catgaggaca acgacatgat gcgaccctac gacgtcgtct aacgtgcatc ggcacgcgct
    12361 cgtcaccagg ctgtagcgcg cggttaggag ttgcgcactt gtaaagacag ggcaggatgc
    12421 cctgtccttt ctacgaagtt gatcctgtac gccgccaata gtgtggaaca aggagcctga
    12481 gcgggcaatc tgccggtcgc tgaatagcca atctgcgcct gctagatagt caaggttgct
    12541 catgacgcgc tgtgctccct accagacaat gtcgagtatg acgcaggccg ggttcgatga
    12601 ttctcccccc tcccatgaag tcgacctgcg tcacattcga tatcggatta gtgaggactt
    12661 tccgagtaac cgaaaccttc gaagagagtc gacaagaaat actcccagag gggagcgagg
    12721 aggcacgaga agatgcttgg cccattcagg cgtttctggg atatgcagag cgagatgaac
    12781 cgcatgatgt cagacgccat cgggagggcg ccgcaggcgt cgatcaccgg agcgggatgg
    12841 acgccgtcag tggatgtcgt ggccagggaa ggagacttgg tcatcaaggc ggagcttcca
    12901 ggcatgaagc gcgaggacgt cgaggtcagc tttcaaaacg gtgttctcac gatctcaggc
    12961 gagcgcagag aagaagagga gcacaaagac gccggctacc tcgtaaaaga gcgccgctac
    13021 ggatctttca gcaggagcat gacccttccc gagggaatag acgaacaaaa gatcaaggct
    13081 agcttcaacg acggcttgct ggaggtcatg gtgcaaggcg caggcgcgca ggtcgcagaa
    13141 gggccgaggc ggatcgagat cggagactag gagcccggca catggaggtc tgcgacctct
    13201 tacatctgtc tactcgaagt agtcctcagc tttggtgatg aaggcttcca tatcgtcagg
    13261 gacttcatcc tctgggtaga tggcctcgac cggacattcc ggctcgcaag ccccacagtc
    13321 gatgcactcc tcagggttta tgaggaactg gtcaccggca tcgtagatgc agtccacggg
    13381 acagacctcg acgcagctct ggtccttcgt gccgatacac ggctcggtga tcacataggt
    13441 caatggctct cctctcccgg cctaccttgg gggtagttta cacccgtccg gcgaagcctg
    13501 gcacacttcg tttacgatat tgtcccggcg cgtgatccgt agctgaggca cgctgtagag
    13561 gccattgaac gaagccttgg ttgacagggc ggtactgcat tcgcgcgtgc gaactgacat
    13621 tctaccgccg agcgcggcct ctctacttca ccagcactcc cgccagctcc atcaactcca
    13681 cgtccagcgc cttcttcttt gcgaccacct cggccagcgg cgttgccact atctccgagc
    13741 ccatcgtccc aaccattagc c
//
LOCUS       DATAIR010000558         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_339674, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000558 DATAIR010000000
VERSION     DATAIR010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339674"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VHM69_13235"
     CDS             complement(<1..726)
                     /locus_tag="VHM69_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX2741403.1"
                     /translation="MEFPKVVKIRQKFPRPRVEDVDAALREQLGREEISSSIRPGMSV
                     ALTAGSRGIAEIDEVLRSLVAILKEMGAEPFIVPSMGSHGGATAEGQVEILESLGVTE
                     EFCDAPIRSSMEVVELGETERGVPVYMDRIASEADGVVLVNRIKAHTDFRSSIESGLM
                     KMASIGLGKHKQALALHGHGVEGIRDFMVEVGDKVLESGHILFGVATVENAYDEPAII
                     EAIPAAKIHDREAELLAEYMEMMP"
     gene            complement(729..1727)
                     /gene="pdxA"
                     /locus_tag="VHM69_13240"
     CDS             complement(729..1727)
                     /gene="pdxA"
                     /locus_tag="VHM69_13240"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX2741404.1"
                     /translation="MTEPLIAVTMGDPAGIGPEIIARNFAEEDFRERNRAIVVGDATI
                     LERAVRLLDLPLRANVVEEPEDAKFEPGTVDVIPETDLPDDLPFGELDARAGDAAFRY
                     VARATALASAGRVGAIATAPLNKEAMHLAGHKYPGHTEILAQLTDTKDYAMMLVTDEL
                     KVIHVSTHVSLREAIERARPERELAVIRLAHESLRKLGVENPRVAVAGLNPHAGENGL
                     FGTEDAEQIAPAVAAAVEEGIDATGPHPPDTVMMRARMGAFDIVVVQYHDQGHIPIKL
                     MGFDTGVNVTVGLPFFRTSVDHGTAFDIAGTGKAEPASLRAALDLARTLAGAEERS"
     gene            complement(1724..>2783)
                     /locus_tag="VHM69_13245"
     CDS             complement(1724..>2783)
                     /locus_tag="VHM69_13245"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX2741405.1"
                     /translation="TDSRTMPAGFAAKRVVDAAHSARGARIVYKKLDSTLRGNVAAEL
                     AAALNAARRDLAIVAPAFPAAGRTTVGGNQLVNGVPVHETEMGNDPSTPVRESHVPTM
                     LADTFSSVGTLRAEDLRDPVRVRQTLRDYECVVADAERDGDLEALVRAVPDPARVLWS
                     GSAGLAHALGSVHPGPYAGEAGMLRTPACPVLVVIGSLSGVAREQLRRLVEEYGDVAV
                     PVRGGESGALERAVDAVREALTTGACAVVHSPEQRSASGGPVLKPLAEVAACLSEEGL
                     LNGLVLTGGSTAVKVSRRLGASGIRLEGEVETGIPLGTLIGPRPYPVVTKAGGFGGPD
                     TLVRAVEALAQCGEEKNT"
BASE COUNT          440 a          976 c          851 g          516 t
ORIGIN      
        1 aggcatcatc tccatgtact cggcgagcag ctccgcctcg cgatcgtgta tcttcgccgc
       61 cggtatggct tcgatgatgg cgggttcgtc ataggcgttt tcgaccgtgg cgacgccgaa
      121 gaggatgtgt cctgattcca ataccttatc cccgacctcg accatgaagt ccctgatgcc
      181 ctcgacaccg tggccgtgca gcgcgagcgc ctgcttgtgt ttgcctagcc cgatagaggc
      241 catcttcatg aggccgctct ctatactgga gcggaagtcc gtgtgcgctt tgatcctgtt
      301 gacgagcaca acgccgtcgg cctccgaggc gatcctatcc atgtaaacgg gcaccccgcg
      361 ctccgtctcc cctagctcga cgacctccat cgaggagcga atcggcgcgt cgcagaactc
      421 ctccgtgacg cccagagact ccaggatctc gacctgtccc tcagccgtcg ccccgccgtg
      481 gctccccatc gatggcacga taaagggctc ggcccccatc tccttgagga tggcgacgag
      541 tgagcgcagg acctcgtcta tctcggcaat tccgcggctt cccgcggtga gcgcgacgct
      601 catgcctggc cgtatcgacg aagagatctc ctctcgcccc aactgctccc gcagcgcggc
      661 atcgacgtcc tcgacgcgcg gccttgggaa cttctgcctg atcttgacca ccttcgggaa
      721 ctccatctct aactcctctc ctcagccccc gccagcgtgc gggcgaggtc cagggctgcc
      781 cggaggctcg ctggctcagc cttccccgta ccagcgatgt cgaaggcggt gccgtggtcg
      841 acgctggtcc tgaagaacgg caggccgacc gtgacgttga cgcccgtatc gaagcccatc
      901 agctttattg gaatgtgtcc ctggtcgtgg tactggacga cgacgatgtc gaacgcgccc
      961 atcctggccc gcatcataac ggtatcaggc ggatgcggcc ccgtcgcgtc tatcccctcc
     1021 tccaccgcgg cggcaacggc cggcgctatc tgctcggcgt cctcggtgcc gaagagaccg
     1081 ttctcccccg cgtgcgggtt gaggccggcg acggcaaccc tcgggttctc gacgccgagt
     1141 ttcctaagcg actcgtgggc gagccggatg accgcgagct ctcgctcggg cctggccctc
     1201 tctatggcct cgcgtagaga cacgtgggtc gagacgtgga tgaccttcag ttcgtccgtc
     1261 acgagcatca tggcgtagtc tttcgtatct gtgagctgcg ccaggatctc cgtgtgccca
     1321 ggatacttgt ggccggcgag gtgcatggcc tccttgttga gaggagcggt cgctattgcc
     1381 ccgactcgcc cggcgctcgc aagcgccgtc gcccgcgcga cgtagcggaa agccgcgtcg
     1441 cctgccctgg cgtcgagctc gccgaaaggc aggtcatcag gcagatcggt ctcagggatc
     1501 acatccaccg tgcctggctc gaacttcgcg tcttcaggct cctcgacgac attggcgcgc
     1561 agcggcagat cgaggagacg cacggcgcgt tccaggatcg tcgcgtcgcc tacgactatg
     1621 gccctgttcc gttctcggaa gtcttcctcg gcgaagttgc gggctatgat ctccggcccg
     1681 atccccgccg ggtctcccat cgtcacggct atgaggggct ctgtcatgtg ttcttctcct
     1741 caccgcactg cgcgagcgcc tctactgctc ttaccagtgt atccggtccg ccgaagccgc
     1801 cggccttggt gaccacaggg tagggcctgg gtccgatcaa ggtccccaaa gggatgcccg
     1861 tctcaacctc tccctctaac ctgatacccg atgcccccag ccgccgcgac accttgaccg
     1921 ccgttgaccc tcctgtcaaa accagcccat tgaggagtcc ttcctcggag agacacgcgg
     1981 cgacctcggc aagtggtttc aagaccggtc ctcccgaagc actcctctgc tccggcgagt
     2041 gaacgacagc gcaagcacct gtggtcagcg cctcccgcac cgcatcgacc gccctttcta
     2101 acgcgccgga ttctccgccg cggaccggga cggcaacatc accgtactct tcgacgagcc
     2161 gccgcaactg ctcccgcgcc acgccgctga ggctgccgat cacgaccagt acaggacagg
     2221 ccggcgtgcg gagcataccc gcctcccccg cgtatggccc cggatggacg ctcccgagcg
     2281 cgtgcgcaag ccccgccgac ccggaccaga gtacacgggc agggtcaggc acggcccgca
     2341 cgagagcctc caggtccccg tcgcgctccg cgtcggcgac gacgcactcg taatctctta
     2401 gagtctggcg cacgcgtacc gggtctcgga gatcctcggc cctcagggtg ccgactgaag
     2461 agaacgtgtc agcgagcatt gtcgggacgt gactctccct gacgggggtg ctcgggtcgt
     2521 tacccatctc ggtctcgtgg actgggacgc cgttgacgag ctgattcccg ccgacggtgg
     2581 tgcgcccggc tgcagggaaa gcgggcgcga cgatggcaag gtctcgccgc gcggcgttca
     2641 acgctgcggc gagctcggcg gcgacgttgc cccgaagggt ggagtcgagc ttcttgtaga
     2701 cgatgcgagc cccacgggct gagtgcgcgg catcgacgac gcgcttcgcc gcgaagcccg
     2761 ctggcatggt gcgggagtcg gtg
//
LOCUS       DATAIR010000559         6738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_314897, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000559 DATAIR010000000
VERSION     DATAIR010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314897"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1055
                     /locus_tag="VHM69_13250"
     CDS             441..1055
                     /locus_tag="VHM69_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2741406.1"
                     /translation="MRQVSLLLTVMAATLVVASGVALAVTRIGTDGPDTLRGTNRDDN
                     LIGKGGDDDLFAMGGNDSLLGGPGRDNVLGGHEIGPGPSGGKKNLLGGPGNDLVNGGS
                     GSDSIEGEEGNDFLIDSPFRKPSKDKIVGGDGNDVIDVLSRPAVKDSVVCGDGFDRVL
                     ADRADLVVSDCERVLVGFGSTVKFYRSIPQRFWDGLPPFPGHES"
     gene            complement(1485..3323)
                     /locus_tag="VHM69_13255"
     CDS             complement(1485..3323)
                     /locus_tag="VHM69_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2741407.1"
                     /translation="MQKHHIAGAAVSVVKDGKLFFAKGYGDADLENGIPVDPERTNFR
                     IGSLAKLFTWTAVMQLAEQGKLDLDADINRYLDFPIPDSYPQPITLKHLMTHTAGFED
                     LYYERLAKDPNDLLPPREWLISHMPARVRPPGDIAAYSSYGTALAGYIVARVSGEPYD
                     QYIQEHILNPLGMVHTTARSSMPPDIRAHTSVGYTYEDGAFQVFPDTSDMGQPALEYS
                     DMGQPALVPAGDMHASATDMARFMIAHLQNGRYSDANIAEARILNKSTMQQMHTMLYT
                     PDPRLLGTAYGFFDFSDNGQRTIGHSGGSDPIFTLLLLLPDQNLGVFVAYNSQGGEDL
                     ILQHLGFQRAFFDHYYPAPALKPVQPPADFAEEAGRFEGTYRITGGTPGTSYTTLEKV
                     GGLLGISTVTISDSGDGTLLFTNPWDEWRFVEVEPLYFRQVDGPFNILFSEDDQGRIT
                     HMFTDYTPMFAFEKLDWYETPGFNMALALGCVLMFVSMIAAVLIRFIRNRRPSGDREP
                     APRGARVAYWIIVGICVLNLLFVIGTVLWGNPVPVFGVSMIYRIVLGVGVLAAVLTVG
                     ALIYSVLAWKRGYWGNAARVHYTLVTVAAVAFVWFLNYWNLLGWRF"
     gene            complement(3887..4075)
                     /locus_tag="VHM69_13260"
     CDS             complement(3887..4075)
                     /locus_tag="VHM69_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741408.1"
                     /translation="MVGEVGAHGERYFAPAAAWAGAEENRVPQRFVFDGPYSYERNPL
                     HVTDFASATNSPAIATHG"
     gene            4199..4822
                     /locus_tag="VHM69_13265"
     CDS             4199..4822
                     /locus_tag="VHM69_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2741409.1"
                     /translation="MASKRKVSNPLALAVMALLEERPMHPYEMVSLMRERGKHESVRL
                     KYSSLYSVVEALGREGLISARETVREGRRPERTIYELTEAGRVEFFDWLRELIGEPMK
                     EFTQFTAGLSFLPALHPDEAVALLEVRVRLLEKEVGEMRSVLDSALEQGLAELFLIEH
                     EHELVLRKAELEWVRGLIQEIKTRTLGGISVWEGFHTDSDPAIQGDD"
     gene            4822..6141
                     /locus_tag="VHM69_13270"
     CDS             4822..6141
                     /locus_tag="VHM69_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2741410.1"
                     /translation="MMGSSTTMDIGRLPRWLGPANRVIIALQHRGVVLGTMRLLSVPG
                     RKSGKMRTTPVSPLTVEGERYIVGGLESSDWVKNARVAGWGILARGRKEEKVSLVELP
                     VEERASILREFPRRVPHGVQFFHQVYGVSADPEEFAALAPHCPVFRISAAPEGCATGS
                     VVSRDGTTIGYRRIGHGPGVVLLHGAASSGYNHMQLAEALSDAFTVYLPDRRGRGMSG
                     PYSEDHEIQKDIEDLDALLTETGARDVFGVSSGGIICLQAALALPAIRRAAIYEPPLV
                     SPGPASMDLLTRYEKEIGQGKVAASLVSAMKATRMGGPIFNAMPHRLLESFVRMGINR
                     EDKRGSGDYVPMKEIAPTLRYDFRIVAETSENVESFEGIRAEVLLLGGSRSPAYMKAS
                     LEVLEKILPHATRIELPGLDHAASWNSDRGGRPYRVAQDLRRFFARQ"
     gene            6392..>6738
                     /locus_tag="VHM69_13275"
     CDS             6392..>6738
                     /locus_tag="VHM69_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX2741411.1"
                     /translation="MTTQTSREGATDYTADEEAIRDLFRQLLDDWGRGDGDAYGSRFT
                     EDADYVAFDGTHTKGRSDIAASHQQLFDKYLKGTRLTGRIESVRFLSPDTALVHAVGG
                     TVMRGKTRPSPER"
BASE COUNT         1439 a         1970 c         2021 g         1308 t
ORIGIN      
        1 ccgggcggcc tgcgggcgac gccaaggcgt ccggccgacg agtcacttgc aaggcaccct
       61 tggggcccac gtggaaggag ggtcaggccg ctccgaggca tcgcccacag ccattctcca
      121 ggatactcac agcgtggaag gaaacagcct gttgaaacac tcctttggta cccgctttcg
      181 ccccagattg ggcaccaaat gcgttggttt tgggtctttt cgagcacgat ttgtggtcgc
      241 aacctgctca atgccgacct tttcaacaca ctggggtgtt cggcgaactt cggcctgagg
      301 gggttctcga atattggcaa agaatgtagc aatgaatagt acatttgagt gatacgctgc
      361 accgttccag aggttatgct gggatcgtca caaaggaagg cgtggtttgt gatatctcgt
      421 cttgaagaaa ggagcgaagg atgcgacagg tcagtcttct gttgacagtg atggccgcaa
      481 cactggtcgt ggcaagcggg gtagccttgg cggtcaccag gataggcacc gatggccccg
      541 acactttgag gggaaccaac agggacgaca accttatcgg caagggcggc gacgacgatc
      601 tcttcgccat gggaggcaac gacagccttc tgggcgggcc gggcagggac aatgtgctcg
      661 gcggccacga aatcggcccc ggtccctccg ggggtaagaa gaacctactt ggcggtcccg
      721 gaaacgatct ggtaaacggc ggcagcggct ccgactccat agagggcgag gaaggtaatg
      781 acttcctgat cgatagcccg ttccgtaaac cttcgaaaga caagatcgtg ggtggggacg
      841 gcaacgacgt catagacgtc ctcagcaggc ctgcggtcaa ggactcagtc gtatgcggcg
      901 acggcttcga ccgggtgctc gccgacagag ccgacctggt cgtgtccgac tgcgagagag
      961 tgctcgttgg ctttggttct actgtgaaat tctacagatc cattccgcag agattctggg
     1021 acggtctgcc accttttccc ggacacgaga gttagcgtct aacccaaaag ggggattaga
     1081 gaggaaggag ccggaacgaa tatacgctcc gacctttcct tcttgcccta tccacccagg
     1141 tgcgtggaga aactgtttgg atattcgagg taagtgccga atcggggcta caaaagtaca
     1201 agttctaccg ccgtgaccgc aacgaggaac ggcaccaccc gcgtgacacc gagtgcgaga
     1261 ggatccgact cgaaatcggg tgccaccagc gggaaaacga tccagctcag gaagccaatc
     1321 ggggcagccg cgagtgccca gatgcccagg aactgtgcaa gagaatactg ctcaacgcgt
     1381 ctctcactcg gctgcccttc tactgtggat tccaattcga ctccggcgcg cgtagtctgc
     1441 ccttccgcct ccctttcatc gatcctcgtt acgaaacttc gccgctagaa cctccaaccc
     1501 aagaggttcc agtagttcag gaaccacaca aaagcgaccg ccgcaaccgt caccagcgtg
     1561 taatgcacgc gggcggcgtt gccccagtag cctctcttcc aggccagcac actgtagatg
     1621 agcgcgccga ctgtcagcac ggcagccaaa acacccactc ccagcacgat tctgtagatc
     1681 atcgagacgc caaacaccgg caccggatta ccccacagca ccgtgccgat cacgaacaac
     1741 aggttcagaa cacagatccc cacaatgatc cagtaagcca cgcgggcgcc gcgaggcgca
     1801 ggttcccggt caccgctcgg gcggcggttc cgaataaagc ggatcaatac tgcggcgatc
     1861 atggacacga acatcaacac gcagcccaat gccaatgcca tgttgaaacc gggcgtttcg
     1921 taccagtcca gcttttcaaa agcaaacatc ggagtatagt cggtgaacat gtgggtgata
     1981 cgaccctggt cgtcctcact aaagaggatg ttgaacggac cgtcaacctg gcgaaaataa
     2041 agcggctcca cttcgacgaa tcgccactcg tcccaagggt tcgtaaagag cagcgtgccg
     2101 tcgccggaat cgctgattgt aactgtgctt atgcccaaga gtcctcccac cttttcgagg
     2161 gtggtatatg aagttccggg tgtacccccc gtgatgcgat aggtgccctc gaaccgaccg
     2221 gcctcctccg cgaagtccgc cggaggttgt actggcttga gtgcaggagc aggatagtag
     2281 tggtcgaaaa acgctctctg gaacccgaga tgctgaagga tcaggtcctc accaccctgg
     2341 ctgttgtagg ccacaaatac tcccaggttc tggtcgggca agagtagcag cagagtaaag
     2401 atcgggtccg atccaccgct gtgcccgata gttctctggc cgttgtcgct gaaatcgaag
     2461 aagccgtagg cggtgcccaa aaggcgcggg tcgggagtgt acaacatggt gtgcatttgc
     2521 tgcatcgtgc ttttgttcag gatgcgcgct tcggcaatgt ttgcatcgct gtagcggccg
     2581 ttctgcaggt gcgcgatcat gaaacgtgcc atgtccgtgg cgctggcgtg catatcgccg
     2641 gcaggcacta gcgccggctg acccatgtcg gagtactcta gcgccggctg acccatgtcg
     2701 gaggtatcgg ggaatacctg aaaggcgcca tcttcatacg tgtatcccac cgatgtatgt
     2761 gcacgtatgt ccggcggcat cgacgaccgg gcggttgtgt gcaccatgcc cagtgggttc
     2821 aggatatgtt cctggatgta ctggtcgtag ggctctcccg atacccgcgc cacgatgtag
     2881 cccgccaggg ccgtgccata gcttgaataa gccgcaatgt cgccgggcgg acgaacccga
     2941 gccggcatat gcgagatcag ccattcgcgt gggggcagca ggtcattggg atccttcgcc
     3001 agcctctcgt aatacaggtc ttcgaaccct gccgtatggg tcatcaggtg cttgagagtg
     3061 atcggttgtg gatagctatc cgggatagga aagtcgagat agcggttgat gtccgcgtcc
     3121 aggtccagct ttccctgctc agcgagctgc ataaccgccg tccaggtgaa cagcttcgcg
     3181 agtgagccaa tgcgaaagtt cgtccgttcg gggtcgacag ggatgccgtt ctcaagatcg
     3241 gcgtcaccat agcctttggc aaagaatagc ttgccgtctt tgacgacgga gacggctgct
     3301 ccggcgatgt gatgcttttg catctccctt cctaattcct catccaggaa ggcctccagt
     3361 tcggcacgat cggaggggcc ttgttgttgt gatggcgtag tggatacatt cctgccctgg
     3421 gctgccctgt tttcctggcc cgtggctgct ccgctagtcg atagcactat cactagagcc
     3481 aacacaatca gcatagatat cgccatgaat ctcttgataa tgagcaagtg attcatttat
     3541 cctttttccg aaagtttgct cgtgccaggg taaggttgac tctgttgtgg gctcttggca
     3601 tgacacatat tgagcatact accaggcagc tttgatcaac gtgtcacgac gtgcagctac
     3661 gaggtttagt caccaacttc ggggaagtaa aggtcccaga agcccgtatt caagatggta
     3721 catatcctta actcttggtc gcctcagttc ctcaaggcca aacacaatac ccagcccacc
     3781 tgaatagaga tacatcgtag cgtatacaaa ccagtgcttt cccacacccg cgactgcgca
     3841 gcgaatgtgg gtgccctatc actccgtcgt taagccctcg tcaaatctaa ccgtgagtcg
     3901 cgatcgccgg ggagttggtc gcggaagcga agtccgtgac gtggagagga ttccgctcgt
     3961 aactgtacgg tccgtcaaag acgaaccgtt gcggcaccct gttctcctcc gccccggccc
     4021 acgccgcagc gggcgcgaag taccgctccc cgtgagcgcc cacttcacca accatcttcc
     4081 attttgaatc cagccaccat cccaagattt tctcaggaaa ttccgcacga cccttgacac
     4141 caggccactc cgagtatata gtcgatctcg attaaactgg atcgactaaa ggatattaat
     4201 ggcgtcgaag agaaaagtat ccaacccgct ggcactggcg gtaatggcgc tcctcgagga
     4261 gcggccgatg cacccttacg agatggtctc gctgatgcgt gagaggggca agcacgagtc
     4321 ggtcaggctc aagtacagtt cactttactc cgtggtcgag gcgctgggtc gggagggttt
     4381 gatctcggcc cgcgagacgg tgcgcgaggg gcgtcgtccg gagcgtacga tctacgagct
     4441 gaccgaggca ggacgcgttg agttcttcga ctggctccgc gagctcattg gcgaaccgat
     4501 gaaggagttc acccagttca cggcggggct ctccttcctc ccggcccttc accctgacga
     4561 agctgtcgcg ctgctcgaag ttcgggtacg gctcctcgaa aaagaagttg gggagatgag
     4621 gtctgtcctc gattcggcgc tggagcaggg cttagccgaa ctcttcctca tagagcacga
     4681 gcacgagctc gtgttacgca aagccgagct cgagtgggtc cgcgggctca tccaggagat
     4741 caagaccaga acgctgggag ggatctccgt ttgggaaggc ttccacacgg actcagatcc
     4801 ggcgatacaa ggagacgact gatgatggga tcaagcacga cgatggacat cggccggttg
     4861 ccgcggtggc tgggtccggc caaccgtgtg atcattgccc tccagcaccg tggcgtggtc
     4921 ttaggcacca tgcgcctgct ctcggtgccc ggccgcaaga gcggcaagat gcgcacgacg
     4981 ccggtctcgc cgcttacggt cgaaggcgag cgctacatcg tgggcggtct cgaaagctcc
     5041 gactgggtca agaacgcgcg ggtggcaggg tggggaattc tggcacgcgg ccgcaaagag
     5101 gagaaggtct cactggttga gctaccagtc gaggaacgcg cctccattct cagagagttt
     5161 ccgcggagag tgccgcacgg ggtccagttc ttccatcagg tgtacggcgt ctccgccgac
     5221 cccgaggagt ttgccgcgct ggcgccgcac tgtcccgtgt tccgtatatc ggccgcgccg
     5281 gagggttgcg cgaccggctc cgtggtctcc agggacggca cgaccatcgg ataccgtcgg
     5341 atcggccacg gtcctggtgt cgtgctgctg cacggcgccg cgtcgtcggg ttacaaccac
     5401 atgcagctcg ccgaggccct gtccgacgcg ttcaccgtct acttgccgga tcgccgcggt
     5461 cgcggcatga gcggcccgta cagcgaggat catgaaatcc agaaggacat cgaggatctg
     5521 gacgctctcc ttaccgaaac gggcgctcgc gacgtcttcg gagtcagttc ggggggcatc
     5581 atctgtctcc aggcggcgct cgccctgccc gccattcgca gggccgccat ctacgaacca
     5641 ccgttggtgt cccccggtcc tgcttcgatg gatttgttga ccagatatga gaaggagatc
     5701 ggtcagggca aggtggccgc atcgctcgtc tcggccatga aagcgacccg gatgggcggc
     5761 cccatcttca acgcaatgcc tcaccggctg ctcgagtcgt tcgtccgcat ggggataaac
     5821 agggaggaca agagaggatc cggcgactac gtcccgatga aggagatcgc accgacgctg
     5881 cgctacgact tcaggatcgt cgccgaaacg agcgagaatg tagaaagctt cgagggaata
     5941 cgcgccgagg ttctcctgct cggcggaagt agaagcccgg catacatgaa ggcgtcgctc
     6001 gaagtcctgg agaagatctt gccacacgcc acacgtatcg agcttcccgg actcgaccac
     6061 gccgcttcgt ggaactccga cagaggcggc aggccgtatc gggtggcaca ggacctgcgc
     6121 cgtttctttg ccaggcaatg aaagtcgttc tcgccgccca cgccgtcccg tactccggtc
     6181 gaggaggttg atgtgaagta agagaagacc cccggcagag gtgccgccac acctcgtcgg
     6241 gggttgcgat ccccgagcaa ccagtcccgc ggagcgagac ctgccacccg aagcatgcgc
     6301 cataaaggat agcaggtcga gctccaccgg acggggccct gagaacaggg ctctgcatac
     6361 cggaatcaac gaaaaaggag ttcgaccgac catgactacc caaacctcgc gcgaaggcgc
     6421 gacagactat acggccgacg aggaagccat ccgggacctc ttcaggcaac tgctcgacga
     6481 ttggggcagg ggtgacggcg atgcctacgg atctaggttt acggaggacg cggactacgt
     6541 ggcgttcgat ggaacccaca ccaaaggacg gagtgacatc gccgcttcgc accagcaact
     6601 cttcgacaaa tatctcaaag gcacgcgact cactggccgg atcgagagcg ttagattcct
     6661 gagtcccgac accgccctgg tacatgcggt cgggggcact gtgatgcgcg gaaagacgcg
     6721 gccctctccg gagcggga
//
LOCUS       DATAIR010000560         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_63201, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000560 DATAIR010000000
VERSION     DATAIR010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63201"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHM69_13280"
     CDS             complement(<1..186)
                     /locus_tag="VHM69_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741412.1"
                     /translation="MREIDRRYTPETGSPGIGHLLERARRKRGVSLEDAERATMIRKD
                     YLQRLESDDHSTMPEPIY"
     gene            complement(217..561)
                     /locus_tag="VHM69_13285"
     CDS             complement(217..561)
                     /locus_tag="VHM69_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741413.1"
                     /translation="MRILLANEPRAYREAFADAFRSLRPHAQVVTLEPEALEAEVLRL
                     HPDVVVCSRTTPAIRAATRSWMEVRIEGELLILRTSHTGRSYDTNPGLETLLAFLDRT
                     EKDSRSHRANAS"
     gene            complement(564..1799)
                     /locus_tag="VHM69_13290"
     CDS             complement(564..1799)
                     /locus_tag="VHM69_13290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2741414.1"
                     /translation="MNAWLKRNEPSHEGNRIEDHSESDRTRGLTYSSSESPPGSGLSR
                     DSTTAVAPHFDLRMKAVSTALYAVGALAVLLVIHGGWLQLSDTNVQALDRVVLAGGVL
                     LWSMWLFPWQRFGRNLLLVPISLGLALATLVVYFSGGWHSPFSVFYLFIVVFCAGYFS
                     ARVAALCVAVTLLASLTPQLYVPNPALLVEQLIVEIPTYLGLALVCRYAVQERTRLQF
                     QQDSEQIRDLEERLWHEASLDPLTGLFNRGRFETRFNEEFERAKRTGERFMLLFVDID
                     DFKDVNDNHGHRMGDEALKLVAEVLQSTSRRIDVVARHGGDEFMVMLPGASLPEAHRF
                     FERMRKRVAERSDRTLGLDLRLSAGAVLCPGYSTDPASLLDAVDEAMYRAKRRGKNRM
                     FAALSLTPADRRRAFQPEE"
     gene            3180..3371
                     /locus_tag="VHM69_13295"
     CDS             3180..3371
                     /locus_tag="VHM69_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741415.1"
                     /translation="MAVVRRDGLLVLWPLRLWLSLWPLPHRLLLRCGGVVVGLRRARW
                     LVRSRGAGWFGRLLRLLGR"
BASE COUNT          587 a         1109 c         1190 g          686 t
ORIGIN      
        1 gtagataggc tccggcatcg tagagtggtc gtcgctttcg agcctctgca ggtaatcttt
       61 gcggatcatg gtggcccgct cagcgtcctc cagcgagacg ccccgcttcc gtctcgcgcg
      121 ctccaggagg tgacctattc caggacttcc ggtttccggt gtatagcgtc tatctatctc
      181 tctcaccttc acctccagga cgttcgcaag gcgctcctaa ctcgcgttcg ccctgtggct
      241 tcgagaatcc ttctcggtcc ggtctaagaa cgccagcagc gtctccagac cgggattggt
      301 atcgtaggat cggcccgtgt ggctggtgcg taagatcaac aattcgccct caatgcggac
      361 ctccatccac gagcgggtgg cggcccttat cgcaggggtg gtgcggctgc acacaaccac
      421 atcgggatgc aagcgcagca cctctgcttc gagagcttcg ggctctagcg taaccacctg
      481 ggcgtgggga cggagtgacc ggaaggcatc cgcgaatgcc tcacggtacg cgcgcggttc
      541 gttggcgagc aagatacgca tctttattcc tccggttgga aggcccgccg gcggtcggcg
      601 ggggtgaggg acagggcggc gaacatgcgg ttcttgccac gccgcttggc cctgtacatg
      661 gcctcgtcca cggcgtccag aagggacgct ggatcggtgg agtagccagg gcacaagact
      721 gctcctgcgc tgaggcggag gtccagaccc agcgtacggt ccgagcgttc ggctacccgc
      781 tttcgcatcc gctcgaagaa acggtgggct tcggggagtg aggctccagg caacatcacc
      841 atgaactcgt cacccccgtg acgcgcgaca acgtctatgc gtctggaggt ggactgcagg
      901 acctcggcga ccagcttcag ggcctcgtcc cccatgcgat gcccgtggtt gtcgttcacg
      961 tccttgaaat cgtcgatgtc aacgaagagc agcatgaacc gctcccccgt acgctttgcc
     1021 ctctcgaact cctcgttgaa ccgcgtctcg aaacgaccac gattaaagag gcccgtaagc
     1081 gggtcgagcg aagcctcgtg ccacagtcgc tcctcgaggt ccctgatctg ctcggagtcc
     1141 tgttggaact gcagcctcgt gcgttcctgc acggcgtacc tgcacacgag cgcgaggccg
     1201 aggtaggtgg ggatctcgac gatcagctgc tcgacaagca gcgccgggtt cgggacgtag
     1261 agctgcgggg tgaggctggc gagcagcgta acggccacgc agagcgcggc gacccgggcg
     1321 gagaagtagc cggcacagaa gactacgatg aagaggtaga agaccgagaa gggactgtgc
     1381 cagccacccg agaagtaaac gacgagcgtc gccagggcca ggcccaagga tatcggtaca
     1441 agcaaaaggt tgcgtccgaa gcgttgccag gggaagagcc acatggacca cagcagtacc
     1501 ccgcctgcca acaccacccg gtcgagggct tgcacgttcg tgtctgagag ctgcaaccac
     1561 ccgccgtgga tgaccagcaa gacggccaga gcgccaaccg cgtagagcgc ggtagacacc
     1621 gctttcattc gtaaatcgaa gtgcggggcc accgccgtgg tactgtcgcg ggatagaccg
     1681 gaaccagggg gactctctga agacgagtag gtaagcccgc gcgtgcggtc cgactcgctg
     1741 tgatcttcga tcctgttccc ttcgtgcgat ggttcgttgc gctttagcca tgcgttcatg
     1801 ttttctcctt gctctgagat acgccgggac gtccggtttt ccgaacggtc ggcgcacggc
     1861 cgatgtaggg catcggggcg cgcagaacgc tccctgagca aggaggatca gcccggtcgt
     1921 tcgagagcca gccgcgggac cgttcccggc tggaaaatag cccggaaggc gcggcctcgg
     1981 cccaggcaca ggagggctat gaggcatggg gcgatcacgg cgaataacgg cgcggtgcca
     2041 tcgcccgatg ttccagagcc tgtggcgacg cctcctccta tagtcaatga gagaggcggt
     2101 ggaacctcaa gcgggggccc gcgttgcgtg agacttccct tatgcagcgt ccctgtggaa
     2161 tgatcgtgcg aaccgccggt caatgaacgg agtacttgtt cggtttgttc gatcagggaa
     2221 cctctgtcgt cccgcaaggt atcgagcacg agtttggccg atccgaaggg cgtccgcgac
     2281 cctatttccg ccagctctcc caccggtctc tgcttcccgc ccggcatgcc ggtatccctg
     2341 gccggcgctg ccgggatgag gtccctgccg cgcacgggtc cgacggcgcg cgcgacccta
     2401 tgacggtcgg gtggatgggc ggaggagggc accgcgagcc tcgcggggcc ttcgctacgg
     2461 gcttccatgg ggtttgcgcc ctcatgtccc tctggccggt aggacggttc cacagtgggt
     2521 cggacgttcg cagagggtcg gtcatccact ctagcaggat gatggggaac tgatcgtacc
     2581 ggggcctggc ggtccaaagc ctccccggca accttgttat tgggcggact gcgatccggc
     2641 gcgttgcccg accgggtggc gcccttctct ttgggaggag ccggttcgtt ggtttccgtg
     2701 tcagctttcg gcccggcctg ggtgttgggc tcgggaggtg gttctgtctg ctccgggcta
     2761 cctgtgacgc cctgtcccct gggatgggcg tgttccctgc ccggtgggtc cgccggcttg
     2821 ccgtgacgga ccggggatcc attaccccgg tgcttgcctg tgttctcgtt tccctgggaa
     2881 tggttcggct tcgccgctcc cggcgtggga ccctgggggg tcggacgcga cgggcggccc
     2941 cggttcacct gaccctgttt ggcgtgctca ggacgaggat gatggcgtgc ggtatccggc
     3001 gccgtcggcc tgtgcgccga cttcccatgg ggcgcaaccc gcgaaccggg ctgtctcgca
     3061 acctgactgt ggccacgagc atgccctctt tgaccggcgg gggcggagcg tccattggca
     3121 tgctcgtggg caggccgcga tgatggagcg cggtcggcgc gctcgggctg aggatgacgg
     3181 tggccgttgt tcgacgcgac gggcttctgg tgctgtggcc tttgcgcctg tggctgtcgc
     3241 tgtggccttt gccgcaccgg ctcctgctgc gctgcggagg tgttgtggtc ggccttcggc
     3301 gcgcccgttg gctggtgaga agtcgcgggg ccggctggtt tgggcgtctt ctcaggctgc
     3361 ttggccggtg actctttccc gacgtacaac aacgagccca gcctgaccag aaagcagact
     3421 acaacaagga cgcaccggtt tcgcatggca ccacgcaccg gaccacttcc ggtgtcagcg
     3481 gaaacaagaa gccttctgtg atcctccatt atccccaacg tgccatacgc tccagagacc
     3541 ggcagatcgc cccaccgtgg tgcttcgaac ca
//
LOCUS       DATAIR010000561         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_339794, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000561 DATAIR010000000
VERSION     DATAIR010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339794"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHM69_13300"
     CDS             complement(<1..240)
                     /locus_tag="VHM69_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2741416.1"
                     /translation="MATEISLVLAATFWGLNFAVTKYAAATIPPLSLVALRFTVGGLL
                     LFLVLRLLEPKSRLGKDLLPMGALGCFGVATAQTGF"
     gene            complement(429..1592)
                     /locus_tag="VHM69_13305"
     CDS             complement(429..1592)
                     /locus_tag="VHM69_13305"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2741417.1"
                     /translation="MRRTILLMASMALASLLSSGVAFSQSTSVGDADTLPDEICSSSN
                     PVSAANRPSITVMTRNLYLGADLDPVFQAVAGGDPLGIIQATTTTWQSVQATNFHDRA
                     GALADEIAHSEPLLVGLQEVSLYRTGDPDSFFGNPTLADHVEYDFLKILLNELDQRGL
                     HYKRVTVTRNTDAETPGFTAPGVLRDIRLTDRDVILARTDPPTSMLKLSNRQTGNFAA
                     RVSLPIGTTGQSIDLLRGWGSVDVTLRGQTFRFINTHLEPEGTFSALQVAQGNELLGG
                     PADTSLPVILVGDFNSRADGLGTPTYGNLIGAGFEDAWNITHPGEPGNTFGHDVDLLN
                     TTVDLTQRLDLVLFRHDLCALDADVVGDELDDRTTSGLWPSDHAGVVATLRGE"
     gene            complement(1771..2103)
                     /locus_tag="VHM69_13310"
     CDS             complement(1771..2103)
                     /locus_tag="VHM69_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741418.1"
                     /translation="MVSPEQSHALLAVLRVLTVVFWVAASLFVLTMAASRVTENEAAW
                     LSWTTGVSLLLVIVCSGVIAWAEFRRNPSEDAESGEWTNKMLFYGLVFAITFFVGSLY
                     LPGLYFAP"
     gene            2598..3221
                     /locus_tag="VHM69_13315"
     CDS             2598..3221
                     /locus_tag="VHM69_13315"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX2741419.1"
                     /translation="MTEATGKAGRELERIIYFSDAVFAIAITILVLDIRVPDIPPELV
                     SRELPARLLDLGPKYVSYVISFLVLAIYWQAHHRVFRPITGYDGTLVWLNFLFLMAVA
                     FLPFPTSLLGEYSEEQVSVVIYAANAAVASLLLVAISWYATSGHRLVAPGLDDEEEKT
                     RRLQGLAAPVVFLASIVVSFFSPMAAMYSWLLLIATDPLVRWLRPRQ"
     gene            3395..4654
                     /locus_tag="VHM69_13320"
     CDS             3395..4654
                     /locus_tag="VHM69_13320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741420.1"
                     /translation="MGSGRSVVSVTNRWTVAAAAVVMQSGFGSLYAWSVFRQPLSAHY
                     GANITDVNIAFFVASLVFATSTFGAGFLLRRVGPRFVGIAGGVLFGLGIFLAAFTGES
                     LLLLYLTYGLVAAVGGGFGLIAPIVVLPRWFPDRPGLAYALALVGFGLGTMVSVPVIS
                     ALLSVTGGPFRTFGVLGISYAVLLGSAAWFVRYPSEDGNAVPGSGESGLLQEREQVRK
                     KGSYDLRQALRTWQWYAIWVMFFLNTTVGLAIYSDARAMAGSISGTSAALASAFVVII
                     SMSDTVGRLVWPILSDRIGGRNVFLVMFLLQALAFLLMPSLGAESFVVLCVLASAVTS
                     CFGGGYATMSALSTAYYGLRDIGAIYGSIVLASGIAGFGAPVLLARSADLTGSYDLAL
                     YITGGLMLIGTTIPLALRPPVSSRGSG"
BASE COUNT          966 a         1516 c         1510 g          995 t
ORIGIN      
        1 gaaacccgtc tgcgccgtcg ccaccccgaa acagcccaaa gcgcccatag gcagcaggtc
       61 cttcccgagc ctgctcttcg gttcgagtaa gcgcagtacg agaaacagca acagaccgcc
      121 caccgtgaac cgcagggcga cgagcgacag aggagggata gttgctgccg cgtacttggt
      181 gacggcgaag ttcaggcccc agaaggttgc ggcgaggacc agcgagatct ccgtcgccac
      241 cgcagccctg tccttgaatc cagacacagg agcggattgt atcgtgtgag aaccggaaga
      301 gagcccgaca gaattcgaac atcgtcacac ggtccacgga acgtcccgcc cggccatgac
      361 tcgaagcaac gctacctgtg ggcgcgtact cgtatcctgc cgggcggcaa acgcaaggag
      421 atctagaatc actctcctct cagtgtcgcc acgaccccgg catggtcaga aggccacagg
      481 ccggaagtag tgcggtcgtc cagttcatcg ccgacgacat ctgcgtccaa tgcacacaga
      541 tcatggcgga acagtacgag atccagcctc tgcgtgagat cgaccgtcgt gttgagaagg
      601 tcaacatcat ggccaaaggt attgccaggc tccccggggt gtgtgatgtt ccatgcatcc
      661 tcgaagccgg ccccgatcaa gttgccgtaa gtcggcgtgc cgagcccgtc cgcccgagag
      721 ttgaaatcac cgaccaggat cacgggcagg gaggtatccg ccggtccgcc caggagctcg
      781 ttgccctggg caacctgtag ggcgctgaag gtaccctccg gctcaagatg agtgttgatg
      841 aaccggaacg tctggccgcg cagcgtgacg tccaccgaac cccatccccg caggagatca
      901 atggactgac cggtggtacc gatagggagc gagactcggg cggcaaaatt ccccgtttgc
      961 ctgttggaga gcttcaacat ggaggtaggt ggatccgtgc gggcgaggat cacatctcga
     1021 tctgtgaggc ggatatcccg tagcactccg ggagccgtga atcccggggt ctcggcgtcg
     1081 gtatttctgg ttaccgtcac ccgtttgtag tgcaggccac gctggtcgag ctcattgagg
     1141 agaattttga ggaagtcgta ttcgacgtga tcagccagcg tcgggtttcc aaagaagctg
     1201 tctggatcac cggtgcggta cagtgagacc tcctggagtc caacgagcag tggctctgag
     1261 tgtgcgatct catccgccag tgctccagcc ctgtcgtgga agttagtggc ctgcacgctc
     1321 tgccatgtgg tagtggtagc ctggatgatg cccagtgggt caccgccggc aacggcctgg
     1381 aagacagggt ccaggtcagc cccgaggtag aggttgcggg tcatgaccgt gatcgacggc
     1441 cggttcgcgg cagacacggg gttgctggac gaacatattt catcgggaag tgtatcagca
     1501 tccccgacag aggtgctctg ggagaatgct accccgctgg atagcaatga tgccagcgcc
     1561 atggaagcca tcaacagtat ggtacgcctc atgaggaaac cgccttcctt ggccagggag
     1621 cactgcgtca cgtcacttgt ttcggttcca acatagtcca cagcgatgaa tcggcgatga
     1681 atcgaacgtc tggaaactca aatgaggcag gaaagcaata gccgggacct gctgcaccgg
     1741 ccctctgatt cttctgggcg ctcgcgaagc ctatggtgcg aagtagaggc cgggcaggta
     1801 gagggatccc acgaagaacg tgatggcgaa gacgaggcca tagaacagca tcttgttcgt
     1861 ccactcgccg ctctcggcat cctcagaagg attcctcctg aactccgccc acgcgatcac
     1921 gccggagcac actattacga gcagaagtga tacgccggtc gtccaagaca gccacgctgc
     1981 ctcgttctct gtaaccctgg acgccgccat cgtcagcacg aagagcgatg cggcgaccca
     2041 gaacaccacc gtcagcacac gcagcaccgc aaggagagcg tggctctgct ccgggctgac
     2101 catcccccgc tcctctccat taccttgctg tgggcggtac atctctcacg ctcccgtcct
     2161 cgctctcatg ctgcgtatat cctgcatcgt agagagaggc agcgcatcac ccaaactacg
     2221 tatctttgtc acgtttattt caagaattga gcgaccttgg gatgaaggat ggcattccgg
     2281 gaacgaagtg gtcgtcgctt atattcccct tcaccgtaat cgtcgagaag ccgcccgtgg
     2341 cggatgcttc gataacagcc agagctgcat gtgctcctga tcctcgacat acgggtgccc
     2401 gacataccgg agaacgtggt agccagtgag ctaccgaagg aggctccgct agatcggccc
     2461 cctgtgatct cctcggtggg accatgagtt cctcaccctc actttgccgt cgcccagacc
     2521 tcacgatcga ggcaagcaac gactgtcttc ctctacactg ctggacactg aaaacggact
     2581 tcgaggcagg agaggctatg accgaagcaa ctggaaaggc agggagggaa ctggagcgca
     2641 tcatctactt ttccgatgcg gtattcgcca tcgccataac gatcctggtg ctggatatca
     2701 gggtgcccga catcccgccc gagctcgtct cgagggagct ccctgcccgt ttactcgacc
     2761 tgggtccgaa gtacgtcagc tacgtcatta gcttcctggt gctcgccatc tactggcagg
     2821 cccaccacag ggtcttcagg ccaataacgg gctacgatgg gacgttggtg tggttgaact
     2881 tcctgttcct gatggccgtg gccttcctgc cgttccccac ctcgctcctt ggagagtaca
     2941 gcgaggagca ggtgtcggtc gtgatctacg cggcgaacgc tgccgtggca agtctgctcc
     3001 tggtggcgat ctcctggtac gccacgagcg gacaccgcct tgtggccccc ggcctggatg
     3061 acgaggagga gaagacccga cgcctgcagg gcctggcggc gccggtggtg ttcctcgcct
     3121 ccatagtagt ctccttcttc agccccatgg cggcgatgta ctcctggctg ctcctcatcg
     3181 ccaccgaccc actcgtccga tggttacggc cccgccagta gtagcccagg atgctgcgct
     3241 atgaacagta tggaaacgcg ggcccggaaa ccgtttctgt tcgtctacgg tatcaaggga
     3301 taacattcgg gcagtatgct ggaaatggat gtcggagagt gcacattccc cgacgttggg
     3361 tgaatggact ggcaagttcg gaggatgaga atacttgggt agcggccgga gcgtggtgag
     3421 cgtcacaaac cgctggacgg tcgccgctgc cgcggtagtc atgcaatccg ggttcggttc
     3481 attgtacgca tggagcgtct ttcggcagcc actctccgcg cactacggcg cgaacattac
     3541 cgacgtcaac atcgccttct tcgtcgcgag cctcgtgttc gcaacctcga ctttcggcgc
     3601 cggcttctta ctcaggcgcg tcggtccgcg cttcgtcggc atagctggag gggtgctctt
     3661 cggactcggg atcttcctgg ctgccttcac cggagagagc ctcctgctcc tgtacctgac
     3721 gtacggcctc gtggctgccg tcggaggcgg tttcggactc atagccccca tcgtggtgct
     3781 tcccaggtgg ttcccggaca ggcccggcct cgcctacgcc cttgctcttg tgggcttcgg
     3841 cctcggtacg atggttagcg tccccgtcat ctcggctctt ctctcggtga ccggcggacc
     3901 gttccgaacc ttcggcgtcc tggggatctc ctacgcagtt ctgctcggca gcgccgcgtg
     3961 gtttgtcagg tacccgtccg aggatggaaa tgccgtcccc ggaagtggag agtcggggct
     4021 tctccaagag cgcgagcagg tgcgcaagaa aggctcgtac gatctccgcc aggccctcag
     4081 gacgtggcag tggtacgcca tctgggtcat gttcttcttg aacacgacgg tcgggctcgc
     4141 catctattcc gatgccaggg cgatggccgg ttcgatcagc gggacaagcg ccgctctcgc
     4201 ctcagccttc gtagtcatca tctccatgtc ggacacagtc gggaggctgg tatggcccat
     4261 actctcggac agaatcggtg ggagaaacgt cttcctggtg atgttcttac tgcaggccct
     4321 ggcgttcttg ttgatgccgt cgctgggagc ggaatcattc gtggtgctct gcgttctcgc
     4381 ctcggccgta acgtcttgtt tcggtggcgg ctacgcaacg atgtcggcgt tgagtaccgc
     4441 atactacggc cttcgggaca tcggggcgat ctacgggagc atagttcttg cctctgggat
     4501 cgccggcttc ggcgcacccg ttctcctggc gcgctcggcg gacctgaccg gatcctacga
     4561 cctcgccctc tacattacgg gagggctgat gctgatcggc accaccatcc cactggcgct
     4621 caggccgcca gtctcctcgc gaggatcagg ctagctccgc cgcgttgcgg aagctctcgg
     4681 cgccttcatg tcgaagatcc acaaacaagg tttcccgccg ttggatgaat agccgcagaa
     4741 cgtaggcacc gaccgaagaa gcacagcggg cctggcagga tgcgaacctg cgatacacgg
     4801 ccccggaaac cgttaggaac accctacctt cttgggatga gcgcctgctt ccgcgggtgc
     4861 ggcgttgagt acaaagacgg cggtgcatct cacggaccga gccacatgac tagagaggcg
     4921 gagagcggtg gagttgccgc tccccttgag tacgaagacg aggccgaaag gggtagacga
     4981 cctctcc
//
LOCUS       DATAIR010000562         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_201904, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000562 DATAIR010000000
VERSION     DATAIR010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201904"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..907)
                     /locus_tag="VHM69_13325"
     CDS             complement(236..907)
                     /locus_tag="VHM69_13325"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2741421.1"
                     /translation="MSDHRDTRNLLGPYVMGALEPREEREVEEHLEGCASCREEARDL
                     RLAHESLADLAYTIEATPPDLKGRVVAGIPRRGARRRVPSWVTAVAAAFCVLAVLAAI
                     FTPDPFGGGAVASATLSPTDRAPDAGGEVSIQGTGENMEVRLEAWGLPACKHEQYYEL
                     WLVEGKERVSAGSFTVGSSGQVDVSMNAPSFAGSYPQVGVTSEWDKDPRPSDEKMLSG
                     ELREL"
     gene            complement(900..1457)
                     /locus_tag="VHM69_13330"
     CDS             complement(900..1457)
                     /locus_tag="VHM69_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2741422.1"
                     /translation="MDRVEDEVLVRRVASGDERALSGLYDRYAGLVYGTGMRYLGDRG
                     TAEELVQDVFTSVWRNAAGFDPARASFATWVFRITRNRATDLIRRRRARVRTVEGELL
                     FEPGETDPSGGLSRNFDVAAALSRLSEVHREVLVLAYFHGYSQREISERTGTPLGTVK
                     SRTTAALRALRESMNFDEDRWPADE"
     gene            1672..2694
                     /locus_tag="VHM69_13335"
     CDS             1672..2694
                     /locus_tag="VHM69_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2741423.1"
                     /translation="MVLMTPDTAGANLQAVARRPARGSLTNFFQDLRDLLRYGTIKAT
                     VQEFMRDDALGLAAQLAFYLILAIFPFILFLVAVLDAFSSSSPQFVTELFDYLRRLLP
                     PQVFDLIETYTRNTLRNPDTAPGLLSVGILGTIWAASGAFSALINALNRAYDVQETRP
                     FWKVKGIAILMTLGLSALILIGVLLLIAGPSIGETVAEIFTLGDEFMAAWNIARWPAA
                     LLFMVVTVALLFYFAPDANQPFRWITPGGFVGILLWVIASVAFNLYLSSDFNSYDKTY
                     GSIGTVIILLLYLYISSLTILFGATLNATLVRIKEEVSGEQILKAEPANEKPDIIDEE
                     IQNKDG"
     gene            2749..2838
                     /locus_tag="VHM69_13340"
     tRNA            2749..2838
                     /locus_tag="VHM69_13340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2783..2785,aa:Ser,seq:gct)
BASE COUNT          528 a          994 c          840 g          548 t
ORIGIN      
        1 cacgtgaaga catttcgatg cccgctcgac gcttgtgtgg cgtggattcg ttgcaagtag
       61 ttgcgacttg ctacctacga acgtccggag aattttagga acatttccta atccccttcg
      121 ccctgcggca ccacgaacac gtcgatgtgg tgaatatgat cggcttcgac atcttccggc
      181 ctttcttcgt actgctgaac tcgacgtgca gcgagccaca cgcgccgaag atagtctaca
      241 actcgcgcag ttcgcccgag agcatcttct cgtcactggg gcgagggtcc ttatcccact
      301 cagaggttac gcccacctgc gggtaggaac cggcgaaaga cggggcgttc atgctcacgt
      361 cgacctggcc gctactgccg accgtgaagc tcccggcgct gacacgctct ttgccctcga
      421 cgagccagag ctcgtagtac tgctcgtgct tgcaggctgg tagtccccac gcttccagac
      481 gcacctccat gttctctccg gtgccctgga tgctgacctc gccgccggca tcgggggcgc
      541 ggtcggtggg actcagggtc gctgaggcga cggccccgcc accgaaggga tcgggggtga
      601 agatcgccgc gagcacggcg agcacgcaga aggccgcggc cacagcagtt acccacgaag
      661 gaactcgtcg ccgcgctcca cgccgcggta tccctgccac gacgcgaccc ttgaggtcag
      721 gcggcgtcgc ctcgatggta tacgcgaggt cggcgaggct ttcgtgtgcg agcctgagat
      781 cccgcgcctc ctctctgcaa ctcgcgcacc cttccaggtg ctcctcgacc tcccgctcct
      841 ctcgcggttc gagggcgccc atcacgtaag ggccgaggag gttccgcgtg tcacggtggt
      901 cactcatccg ctggccaccg gtcctcgtcg aagttcatcg actcgcgcag ggcgcgcaac
      961 gcggccgtgg tgcggctctt gacggtaccc agaggcgtgc ccgtgcgctc ggagatctca
     1021 cgctgagagt agccgtggaa gtacgcgagt acgagcacct ccctgtggac ctcggagagc
     1081 cgtgagagag cggccgccac gtcgaagttc cgtgagagcc cgcccgatgg atctgtctcg
     1141 ccgggctcga agagcagttc tccctccacc gtccgaacgc gcgccctgcg gcgacggatc
     1201 aggtccgtcg cccggttgcg ggtgatgcgg aacacccacg tggcgaagct ggcccgcgca
     1261 gggtcgaagc cggcggcgtt tctccacacg gaggtgaaga catcctggac gagctcctcc
     1321 gccgtccccc tgtctcccag gtagcgcata cccgtcccgt agacgaggcc ggcataccgg
     1381 tcgtagaggc ccgagagggc gcgctcgtcc cccgacgcca cccttcgcac cagaacctcg
     1441 tcctctacac ggtccatcca gagccttccc cagccaccgg cgaaagcgga ctctccggtc
     1501 caacgccaca cggcacagat gatatcccgc ccgctatgcg gcgcgagaaa tacagcactc
     1561 cccgacacta actctttcat acagagattt atacgaccgg ccgggcccag cggattatac
     1621 gcccagggtc gggattgtgg ttaaaatgag gcaagttacg agaagggagg tatggtcctg
     1681 atgacacccg atacggcagg ggcgaatctc caggcggtcg cgaggcgtcc cgcgcgaggg
     1741 tcgctcacga acttcttcca agatctgagg gacctcctcc gatacggtac gatcaaggcg
     1801 accgtacagg agttcatgcg cgacgacgcc ctcggactcg ccgcgcagct ggccttctac
     1861 ctgatcctgg cgatcttccc tttcatcctg ttcctcgtcg ccgtgctcga cgccttctcg
     1921 agctccagtc cgcagttcgt cacggagcta ttcgactacc tcaggcggct ccttccgccg
     1981 caggtcttcg acctcataga gacctacacc aggaacaccc tccgcaaccc cgacacggct
     2041 cccggcctcc tctcggtcgg catcctcggc acgatctggg ccgcttcagg ggctttctct
     2101 gccctgataa acgccctcaa ccgcgcctac gacgtgcagg agacgcggcc cttctggaag
     2161 gtgaagggca tcgccatcct gatgacgctc ggtctctcgg cgctcatcct cataggcgtg
     2221 ctactcctga tcgccggccc ctccataggc gagacggtcg cggagatctt cacgctcggc
     2281 gacgagttca tggccgcctg gaatatcgca cgctggcccg cggcgctgct gttcatggtc
     2341 gtgactgtcg ccctcctgtt ctacttcgcc ccagacgcca accagccgtt ccgctggatc
     2401 acaccgggcg ggttcgtcgg catcctgctc tgggtgatcg ccagcgtcgc cttcaacctg
     2461 tatctgagca gcgacttcaa ctcatacgac aagacttacg gttccatcgg caccgtcatc
     2521 atcctgctcc tctacctcta tatctcctcc ctgacgatcc tcttcggcgc aaccctcaac
     2581 gccacgctcg taaggataaa agaggaggtc tccggagagc agatcctcaa agccgaaccg
     2641 gccaacgaga agcccgacat catcgacgaa gagatccaga acaaggacgg gtgagcggcg
     2701 tatcaggcgc gtcgaagacg cggcacgtct ttgatatact cgtgccccgg aggagtggcc
     2761 gagtggttga aggcggcacc ctgctaaggt gttatacctc ttaacggggt atcgagggtt
     2821 cgaatccctc ctcctccgca ccgactacac caacgcctag aggtcaggcc ccgtgatcag
     2881 atagggataa gaggctttcg tctctacttt
//
LOCUS       DATAIR010000563         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_37875, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000563 DATAIR010000000
VERSION     DATAIR010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37875"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VHM69_13345"
     CDS             <1..515
                     /locus_tag="VHM69_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2741424.1"
                     /translation="EDLPDVETVLVPVSGGGLISGVSAAIKLNRPETNVIGVEPELAA
                     DARASLKSGRLVELPADQVSRTIADGLRVQRLGEAPFEHVRAFVDDIVTVTEEEILDA
                     MRRLALRVRLVAEPSGAVTFAAYLFHGEELPDSRLTVAVISGGNVEPGFLAEVLSGSS
                     LEEVRKAATR"
     gene            complement(528..1337)
                     /locus_tag="VHM69_13350"
     CDS             complement(528..1337)
                     /locus_tag="VHM69_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX2741425.1"
                     /translation="MAADNVTLSGLYVYPIKSCAGIPLKRAELSATGLRHDRRWMLVD
                     ETGEFMSQRTHPRMALISVSFSTEGLIVTAPDMPELEIPLRQENGTRIDVRVWGDTNR
                     GALVGEEADRWFGEFLDFPCRLVSKPEDDLRLVDSIYAEDGDQVGFADGFAFLLISEA
                     SLEDLNERLEECLPMNRFRPNFVVRGCPPYAEDGWSRLRIGSVPFRVAEPCPRCAITT
                     VDQNTGVPGKEPLRTLATYRKTELGAVFGRNLIHDAPGTVRVGDLVETTPR"
     gene            complement(1327..1842)
                     /locus_tag="VHM69_13355"
     CDS             complement(1327..1842)
                     /locus_tag="VHM69_13355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2741426.1"
                     /translation="MFERFQPDEDAYERRDRTGTCFVCGIVSRDPDYPKHHVVYEDDA
                     VIAFLNRYPTQYGYTLVAPKEHKVQVADDFTIEEYLGLQRVVHRVAEAVREEVGADRV
                     YLLSLGSNEGNAHVHWHVVPLPPGTPEQQFSAIMLERAGAMKIPEEEIVSLAARLRRR
                     VRRSPETYFGG"
     gene            complement(1922..>2303)
                     /locus_tag="VHM69_13360"
     CDS             complement(1922..>2303)
                     /locus_tag="VHM69_13360"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741427.1"
                     /translation="FQHWHADPDVNPYVLCDGDALIGYGEVWIDETEHEVELGRIIVR
                     PESRSRGVGKRFVILLLERAAQSGFPDAFVRVVPDNHVAIACYRSAGFSLVPESVRER
                     YNRGQPVDYVWMRHPLTDNGSGPP"
BASE COUNT          434 a          726 c          718 g          425 t
ORIGIN      
        1 tcgaagacct gcccgacgtg gagaccgttc tcgtgcccgt cagcggcggc ggcctcataa
       61 gcggcgtctc cgccgccatc aaactaaacc gacccgagac aaacgtaatc ggggtggagc
      121 ccgagctcgc ggccgacgcg agggccagcc tgaagagcgg aaggctggtc gagttaccgg
      181 cggatcaggt ctcccgcacg atagccgacg ggttgcgcgt gcagagactg ggcgaagcac
      241 ccttcgagca cgtacgcgca ttcgtggacg acatcgtcac cgtgacggaa gaagagatcc
      301 ttgatgccat gcgccgcctg gcattgcggg tcaggctggt cgcggagccg agcggggccg
      361 ttacgttcgc agcatatctc ttccacgggg aagagttacc cgactcgcgc ctcaccgtgg
      421 ccgtgatcag cggcggcaac gtcgagcctg gcttcctcgc cgaagtcctc tccggttcct
      481 ctctcgaaga agtccgaaaa gcagctacga gataggaccg acggctttca tcgcggcgtg
      541 gtctcgacga ggtcgccgac gcgaaccgtc cctggcgcgt cgtggatcag gttgcgcccg
      601 aagacagccc cgagttcggt cttgcgatac gtcgcgagcg tgcgcaacgg ctctttgccg
      661 gggacgcctg tgttctgatc caccgtcgta atagcgcatc gggggcacgg ttcggcgacc
      721 ctgaaaggca cgcttccgat ccggagccgg ctccagccgt cttcggcgta aggagggcag
      781 cccctgacga cgaagttggg acggaagcgg ttcatgggaa gacattcttc caggcgctcg
      841 ttcaggtctt cgagcgaggc ctcggagatc aggaggaacg cgaaaccgtc ggcgaagccg
      901 acctggtcac catcttccgc gtagatcgag tcgaccagcc ggagatcgtc ttccggcttg
      961 cttaccagcc ggcaagggaa gtccaagaac tctccgaacc agcggtcggc ctcctcaccc
     1021 acgagcgcgc cccggttcgt gtcaccccag acccgcacgt ctatccgggt accgttctcc
     1081 tgccggagtg ggatctcaag ctcaggcata tctggagcgg tgacgatcag gccttctgtg
     1141 gagaagctga cggagatcag ggccatccgc ggatgggtgc gctgtgacat gaactcgccc
     1201 gtctcgtcga cgagcatcca gcgccggtcg tggcggagtc ccgtggcgct cagttcggcc
     1261 ctctttagcg ggatcccggc gcaggacttg attgggtaga cgtacaaccc actaagggtg
     1321 acgttgtcag ccgccaaagt acgtctccgg agatctgcgc accctgcggc ggaggcgggc
     1381 ggcgagggac acgatctctt cctcgggaat cttcatcgca ccagccctct caagcatgat
     1441 ggcggagaac tgctgttcgg gagtgcccgg cggcaaaggc acgacgtgcc agtggacgtg
     1501 ggcgttgccc tcattggagc ccaacgagag tagatagacc cgatcggccc ccacctcctc
     1561 acgcaccgcc tccgccacgc gatgcacgac ccgctgcaga cccaggtact cctcgatcgt
     1621 gaagtcgtca gccacctgca ctttgtgctc tttcggcgcg accagcgtgt acccatactg
     1681 cgtcggatac ctgttgagga aggcgatcac ggcgtcatcc tcgtagacga cgtggtgttt
     1741 tggataatcc gggtcccgcg atacgatgcc gcaaacgaag caggtccctg tccggtctcg
     1801 cctctcataa gcgtcttcgt cgggctgaaa tcgttcgaac attttcactc gaagttgagg
     1861 atcgggactt gttttacgcg gctcgacaca gactccatca tccaccttaa agtcgatggc
     1921 ttcaaggagg accgctccca ttatcggtca gggggtggcg catccagacg tagtcgacgg
     1981 gctgtccccg gttgtaccgt tctcgtacgg actcgggaac cagcgagaac ccggcgctcc
     2041 ggtaacaggc gatggcgaca tggttgtcag gaaccacgcg gacgaaggca tcaggaaagc
     2101 ccgattgggc cgctcgttcc aggagcagga tcacgaaccg tttgcctaca ccgcggctcc
     2161 ggctctcggg cctgacgatg atgcgtccca gttcgacttc atgctcggtc tcgtcgatcc
     2221 acacctcgcc gtagccgata agtgcgtcac cgtcgcacaa cacgtatggg ttcacgtccg
     2281 gatcggcgtg ccagtgttga aat
//
LOCUS       DATAIR010000564         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_315295, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000564 DATAIR010000000
VERSION     DATAIR010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315295"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VHM69_13365"
     CDS             <1..516
                     /locus_tag="VHM69_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2741428.1"
                     /translation="MEALPESRMILLVRDPRDVVASVLDAAREGGWLYASREGKTGTR
                     RGRVERRPNVFVRERAKVYRRGVESARRAYDAHGGPKVLVRYEDLRSDTMNEMSRIYS
                     TLGIRVDEEELRRAVEKHSWENVPEGEKGRGKFYRKGTPGGWKEDLTPRQVEVVERTT
                     ASLLQIFYPES"
     gene            513..1466
                     /locus_tag="VHM69_13370"
     CDS             513..1466
                     /locus_tag="VHM69_13370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2741429.1"
                     /translation="MSAPKASVVIPTFNAGPGFEETLGRLVAQEAYFEYEILIVDSGS
                     TDSTIEIARRHGASVYLMPQGEFDHGTARNLGISNARGEHVALIVQDALPVDSRWLVA
                     MVENLDEDATAAGVYGRQIPHPGSSPLTRALMDDWPTSGLERREQFAGDPESYRRMSA
                     DTRRKLATFDNVSSCVRRSVWENFPFEATGFGEDLRWGKKAVEAGYKLVYEPRSTVLH
                     SHERGALYDLRRYYADGLVLQDLFGLAPTPNLARLLLNVLLSSAHLYLRLSRNDKRVA
                     PRSLLLAVRYALCSQTGAYLAARRSKSRISARVHEYLIKGV"
     gene            1530..2885
                     /locus_tag="VHM69_13375"
     CDS             1530..2885
                     /locus_tag="VHM69_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2741430.1"
                     /translation="MRILFTVHQFFPESRAGVEVVTLGLARELKARGHEPFVLAAKRT
                     VPHSELRPGQTEDYEFDGIPVRRVGRPEEGLSRPYELNFRNDDMALEVREYVRDVAPD
                     IVHAMHLQGLSASVLPVFKEFGLPVVFTAADFWTVCPVVDLRRHDGVMCKGPDVAHCV
                     RCIASRSADPRLKKAANLLPGAVPRAADLISRTPLSRFSLPLRQVGAVRERPEYIRKR
                     MDLVDHVLAYTRLGRDLLIANGMAEEKIQVSHYGIDTSHVAGASSERRPSSKLRLGFV
                     GTLAPHKGPDILLRAFGMLPPGLDATLSVHGSEEGYESYADELRDLAGDDGRISFRGA
                     FAREELRSVLAGIDVLVVPSRWYENAPGVIFEAFAAGMPVVATDLGGMSEFVRRGRNG
                     LLFGLEDAQDLARQLRRLAEEPGLLPELRAGIGAVKSVGEYADEREQLYNTLLNGRTR
                     Y"
BASE COUNT          583 a          941 c         1014 g          512 t
ORIGIN      
        1 atggaggccc tgcccgagag ccgtatgatc ctgctcgtac gtgacccgag ggacgtggtc
       61 gcctccgtcc tcgacgccgc aagagagggg gggtggctat acgccagcag ggaaggtaag
      121 acgggcacgc gtcgcgggag ggtagaaagg aggccaaacg tcttcgtcag ggagcgggcg
      181 aaggtgtaca ggcggggcgt ggagagcgca aggcgggcgt acgacgccca cggaggaccc
      241 aaggtgctcg tccgttatga ggatctcagg tcggacacca tgaatgagat gagtcgcatc
      301 tactctactc taggcatccg tgtggacgaa gaggagttga ggagggccgt cgagaagcat
      361 tcctgggaga acgtgccaga aggcgagaaa ggcaggggca agttctatcg caaggggacg
      421 ccgggcggct ggaaagaaga cctgaccccg aggcaggtcg aagtggtaga gaggactaca
      481 gcctccctgc tccagatatt ttaccccgaa agttgagcgc gccgaaggca tcggttgtaa
      541 tccctacatt caacgccggt cctggcttcg aggagacgct cgggaggttg gtcgcgcagg
      601 aggcttactt cgagtacgaa atcctcatcg tggactcagg ctcaacggac agcactatcg
      661 agatcgcccg gcgccacggc gcttccgtct acctcatgcc gcaaggagag ttcgaccacg
      721 ggacggcgcg caacctgggc atctcgaacg cgaggggaga gcacgtggcc ctgatcgtgc
      781 aggatgcctt gcctgtagat agcagatggc tagtggccat ggtcgagaac ctcgacgagg
      841 atgcgaccgc cgccggggtg tacgggcgtc agatcccaca ccctgggagc agcccgctga
      901 cccgcgctct gatggatgat tggcccactt ccggactaga gcggcgcgag cagttcgccg
      961 gagaccctga aagctaccgc cggatgtccg cggacacgcg gcgcaagctc gcgaccttcg
     1021 acaacgtgag ctcctgcgta cgacgctcgg tgtgggagaa cttccccttc gaggccacgg
     1081 gcttcggaga agacctcagg tggggcaaga aggccgtcga ggctggttac aaactcgtgt
     1141 acgagccgcg ctcgaccgta ctccactccc acgagcgcgg cgccctctac gatttgcgcc
     1201 gctactacgc agacgggctg gtcctccagg atctcttcgg actcgctcct acgccgaacc
     1261 tggcacggct gctcttgaac gtgctcctct cctccgcgca cctctacctc cgcctcagcc
     1321 ggaacgacaa gagagtggct ccacggtccc tgctgctcgc cgtgaggtac gccctctgct
     1381 cccagacagg cgcctacctg gcggcccgca ggtcaaagtc ccggatctct gccagagtgc
     1441 acgagtacct gataaagggt gtctagcgat tccgtataat ctctggtagt gcatcaagga
     1501 cgctgcgcaa agtcactcgg ctctcgacgg tgaggatcct ctttaccgtc caccagttct
     1561 tccccgagag ccgcgcgggc gtggaggtcg tgacgctcgg gctggcgcgg gagctgaagg
     1621 cccgcggcca cgagcccttt gtcctagccg ccaaacgcac cgttccccac agcgaactga
     1681 ggcccggtca gacggaggat tacgagttcg atggcattcc cgtccgcagg gtcggccgcc
     1741 cggaggaggg actgtcccga ccatacgaac tcaacttcag gaacgacgac atggctctgg
     1801 aggtacggga gtacgtgcgg gatgtagctc ctgacatcgt ccacgccatg catctgcagg
     1861 gactctcggc gagcgtgctg ccggtcttca aggagttcgg gttgcccgta gtcttcacgg
     1921 cagccgactt ctggacggta tgccctgtcg tcgacctgag gaggcacgac ggcgtaatgt
     1981 gcaagggacc cgacgtagcc cactgcgtcc ggtgcatagc gagccgaagc gccgacccac
     2041 gcctgaagaa ggcggcgaac ctcctccccg gagcggtccc gagggctgct gacctgatct
     2101 cccgcacgcc gctctcgcgc ttctccctcc ctttgcgcca ggttggggcc gtgcgggaac
     2161 gccctgagta cataaggaag cggatggatc tcgtggacca cgtcctcgct tacaccaggc
     2221 tcgggcgcga ccttctcatc gccaacggca tggcggaaga gaagatacag gtctcccact
     2281 acggcatcga cacctcccac gtcgccgggg cctcctccga gcgaaggcct tcctcgaagc
     2341 tccggctcgg gttcgtcggt accctggctc cgcacaaggg tcccgacata ctcctgcggg
     2401 ccttcgggat gctaccgccc gggctcgacg cgacgctctc cgtccacggg agcgaggagg
     2461 gctacgagtc ctatgcggat gaactgcgtg atctcgccgg ggacgacggg cggatctcct
     2521 tccgcggcgc tttcgcgcgc gaggagctga ggagcgtcct ggcggggatc gacgtcctcg
     2581 tggtgccgtc ccgctggtac gagaacgccc cgggcgtgat cttcgaggcc ttcgccgctg
     2641 ggatgccggt ggtagcaacg gacctcggag gcatgtccga gttcgttaga cgtggacgga
     2701 acggccttct cttcggcctt gaggacgcgc aggacctcgc caggcaactg cggcggctgg
     2761 cagaggagcc tggcctgctc ccggaactgc gcgcggggat aggcgcggta aagagcgtcg
     2821 gcgagtacgc tgacgagagg gaacaactct ataatacgct cctgaatggg cggacaaggt
     2881 attaaggaga ccgtggagag cgtgtcagca tgaaggtcct actaacaggc tcgaacggcc
     2941 agctcggaag ggaactggcc aggatacttc cggagcgagg ccaggaagta gtcgctctag
     3001 acaggaagag cctcgacgtg gccgaccctg aggccgtaga gcgggtcgtt
//
LOCUS       DATAIR010000565         8588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_339908, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000565 DATAIR010000000
VERSION     DATAIR010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339908"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..2070
                     /locus_tag="VHM69_13380"
     CDS             430..2070
                     /locus_tag="VHM69_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2741431.1"
                     /translation="MALGQEQTTSPPGDSTPGGKKRLGRAGVVFAGLGVFLLVFVLYL
                     RTLAPTVLYLNDPRLLDAVMLQMQVSVLGITHPTGYPTYLMLTHLFTYLPFGDPAYRV
                     NLGSAIYAGLAVIVVYAGGLMLSRRVVAAAAGALAFGLGAALWSQAVIAEVYTLNALL
                     VSVTIVVLLIWREYRKDRYLLLSAFLVGLCLTNHLTSGLLLPASLLFVALVDWRRLVD
                     FRLILKGAGLFLLGLTPYLYLPVRAAMDPPMDANNPTNFERFWYVISGGNLTGTFFSF
                     GPFELPGRIVFYWEHLLDNTNLLVAMIAVTGAVTMLLKDRAVGIFLAFLFFGWLFHAV
                     ENDIPDVNLYFIPTYLVLSLWAAAGIGALFEEVEALIAGFPRSAKGAILVALSAMVLV
                     LPLLGVRETYAQDDMSGVYQGRKEIEAVAENAAPNATILQHRSSMWYMVLVEKRRRDL
                     TIVDPFFHNLEVSYADLVWPDDIDLATTNRRYGTDDFTGVEAAKKAAKRGRVYVMNQG
                     YAQRGDFEDAGFQVVRVDEYGIIYELVPQGREPYKADD"
     gene            complement(2067..3671)
                     /locus_tag="VHM69_13385"
     CDS             complement(2067..3671)
                     /locus_tag="VHM69_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2741432.1"
                     /translation="MIGIYALLGLAAACSLSACFPSAEGKQPDQEQEVPQPPNVILIL
                     TDDLAANDLNSDTLQHMPNLKSLLMDKGTTFDNAFVTNSLCCPSRATILRGQYTHSHQ
                     ILSNEPPRGGFQKFRFLGHENSTMATWVKDQGYRTAFFGKYLNGYNETYVPPGWDEWY
                     AVTGNYLSHMLNENGHMVSYDPESYYDTDVLSDKASDYITRTAGADPPFFTADRPFLM
                     WLGTKAPHQPANPAPRDVEALPGVSLPRPPSFDEMDVSDKPGWVSDNPPLSLEQKKYM
                     EELHRKRLQSMLAVDDMVGDLVKALHESGELDNTYIFFTSDNGFHLGQHRLGAGKWTA
                     YEEDIRIPLIVRGPGVPEGRTLHQMVLTNDLAPTFADLAGARTPNFVDGRSIQPLLTE
                     DPTPLEDWRQRFIIESVAERSGVAQPPFINESTVAPLLTGDPLPNNWRKTSAASAQSS
                     EEWGRPWMKALRTQNYLYVDYKTGEHELYDLRRDPYELHNQFATASPELKQRLEAELD
                     ALRQCTAAECYAAEGGQLEGVAPDGR"
     gene            3875..5929
                     /locus_tag="VHM69_13390"
     CDS             3875..5929
                     /locus_tag="VHM69_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741433.1"
                     /translation="MISDLIQALPAALLVGVLPGWFWTRCLLASGDHAERLAYTIALS
                     ITLVPTAALAQTYLFTTGVTFAVTVASVALVFLTGLAAYLLLGPAKGPEEPVLRPPSR
                     PGLPTLIPLVVALALALVMLLGIVPGEWLMIPIAILVLAAGIAFLWTGREAPPSEEKR
                     VPAAALYAMLCVVLLLVLARGYLGPVINGWPFPRGVDRYEHAVMAGMMMREGSNESFM
                     LYPPGFHLLTAMLSRLCGFEPLEVFPILAPTLPLLCALACYALAKRLWGPEYGVAAAL
                     TSGLLLGGTYMHFEEARYPNFIGEYFLIVLAVAALIWLYAAPSARGGLLLALLGSSTV
                     LYHQIAGYSLAVLLVFVSVLFLPYLLLRDHRRGVYLLLSLALLGFFSVLYAWDTYDLP
                     SLLAGLLGGSETGKGGEAVAMAIGTKPGNGPGHFLMTMSHPVLWLGLLGLPLMLAGPN
                     NGEDKSATLARLTLLVWTLLLFFGSLTSYSGFPDRFERDLGVPLALLGALAIVTLLRS
                     RWSPHRTVFGAGLLAVVLTATLVGAQSILNLEEAAGASTRLKDRPPPGEVVAAGTWLE
                     EHNEGGSIVATPYLEYVPSRAMLAMGGYTRMQSYDAARILRARDLPPFGAGPMWEALW
                     ILKHPAGEHTDQLLQKYDVRYVVFHKRYPGIDWRPFASRTGLYRTAFENQGVIIFAPR
                     ED"
     gene            6024..8138
                     /locus_tag="VHM69_13395"
     CDS             6024..8138
                     /locus_tag="VHM69_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741434.1"
                     /translation="MVLDLIRALLAALVVAVAPGWFWAGLLRTSGDYAERLTYSAALS
                     MALVPATALIPTRLLGLSLTLPTALACALVVFLAGLGTYLRFGAAKGSDEPIFPAPVA
                     TLSVPALALLVPAFGLAFGVMIGAIPGVPVAIPFTSAVDPGPRVMATVALLVCAAGTV
                     HLFMSRRTDGSEAVESPVLPGPILRRLALPAVLLLAIARGYLGPVLHDWPFVRGVDHY
                     SHAVMADRMMSVGRIEPYLIYPPGFHTLTASISRLSGLEPLEIFPVLGPAVLLLPTLA
                     LYALARRLWGWEYGVGAALLSVLMGGSYYYFNDAMYPNLVTSQFLLVLALASLAGLYK
                     APSARNGVLFALLGSSVVLYHPVASMYLAVLLAVVGAYFIVPLLFRDRRTGLALLLSF
                     ALLGFLSVLYAWNTYHLGQALAGLVDRSGESNTTEAVKMAVGTQAPYGIDLQIGTMVS
                     QPVAWLGLLGVFLLVGDGRRRMGVPAAIAHITLFLWTLLLFVGSRAALTGFPQRFGRD
                     VGVPLALLGALALVTILRSLGTRRRAAAVFVASVAALLCVALVGVQTAQSLKFASGPS
                     LQKTMTPQIEAAGAWLEEHNTGGNIIVSPHVNQVPSRMMLAMGHYSALQSFELWQVAN
                     PRDLPPTGPKPLWDILWLMKHPDNERTDRALSEYDVRYIVLYKNMPDRPTEDYWLDFK
                     SHPKLYRTDFENSDVLIVERRE"
     gene            8202..>8588
                     /locus_tag="VHM69_13400"
     CDS             8202..>8588
                     /locus_tag="VHM69_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741435.1"
                     /translation="MSTGVGFILAVYAASRMFYLISGSLLARIVPTSSFQRVTSDVPS
                     GSMNLWSHWDGEHYVALALGGYLNPPDNVSPAFFPLYPLLMRSFAELFGGPLSQGALS
                     VWGPLLSLLFLPFALYFIYHLALDEWG"
BASE COUNT         1388 a         2787 c         2624 g         1789 t
ORIGIN      
        1 ggccggatcg gggtgaggga ctcgcattcc agctcgatgt ctatcttgcg ccgggagagg
       61 ttgtcgacta tggccacctc gtatccttgc gccgagaggt gcagcgaggt gggccagccg
      121 cagaagccgt cgccgcctag aaccagtacc ttcatttatc gcactccctt gttgctctac
      181 gctactatct tggtttcagg atctaaccta cttgaaccga acgtgccagc caacgattgt
      241 aacggaaaaa cggttgaatg agtactgaat cgccgtaaag gctctgttca aatttctccc
      301 gcggtccctc acgccgggca acatacccgg ccggcgccat attaacgcag tttaatgcaa
      361 attaacatca ggtgttccat ctgggtacaa tagcctccgc ccacgaagag caaaggacgt
      421 tatgagagcg tggcactagg gcaggagcag acaacctctc ccccagggga ttcgacgcct
      481 ggcgggaaga aacgtctcgg cagggcgggc gtggtcttcg ccgggttggg ggtcttcctg
      541 ctcgtgttcg tcctctacct taggaccctg gcgccgacgg tcctctacct caatgatccc
      601 aggctgctcg acgctgtgat gttgcagatg caggtctcgg tactcggcat cacgcacccg
      661 accggctacc cgacctacct gatgctgacg cacctgttca cctacctgcc tttcggggac
      721 ccggcctacc gcgtgaacct cggttcagcc atctatgcgg ggcttgccgt catcgtggtc
      781 tacgccgggg gtcttatgct cagcaggcgg gtcgttgcgg ctgctgctgg cgcactcgcc
      841 ttcgggctgg gggcggcctt gtggtcacag gctgttatcg ccgaggtgta caccctgaac
      901 gccctgctcg tttcggtcac catcgtcgtg ctgctgatct ggcgcgagta ccgcaaggac
      961 cgctatctgc tgctctcggc cttccttgtt ggactctgcc tgaccaacca cctcaccagc
     1021 ggattgctcc tgcctgcgag cctcctcttc gtagccctcg tagactggcg caggctcgta
     1081 gatttcaggc tcatcctcaa gggcgccggg ctcttcctcc tcgggctcac gccataccta
     1141 tacctgcccg tacgggcagc gatggacccg cccatggacg cgaacaaccc gaccaacttc
     1201 gagcgtttct ggtacgtcat aagcgggggg aacctgacgg gcacgttctt ctccttcggc
     1261 cccttcgagc tgccgggaag gatagtcttc tactgggagc atctgctcga caacacgaac
     1321 ctcctcgtgg cgatgatagc ggtgacgggc gccgtcacca tgctcctcaa agaccgcgcg
     1381 gtcggcatct tcctagcgtt cctcttcttc ggctggctct tccacgcagt cgagaatgac
     1441 atccccgacg tcaacctcta cttcataccc acatacctcg tactctcgct atgggcggcg
     1501 gccgggatcg gggcgctgtt cgaggaggtc gaagccctca tcgccggctt cccgaggtcc
     1561 gcgaaggggg cgatcctcgt cgcgctctcg gcgatggtgc tcgtcctgcc gctgctcggc
     1621 gtgcgcgaga cctacgcgca agacgacatg agcggcgtct accaggggcg aaaagagatc
     1681 gaggccgtcg ccgagaacgc cgcgccgaac gcgaccatcc ttcaacaccg cagcagcatg
     1741 tggtacatgg tgctcgtcga gaagcggcgc agggacctga cgatagtcga cccgttcttc
     1801 cacaacctgg aggtctctta cgccgacctc gtatggcccg acgacataga cctcgcaacg
     1861 accaaccgcc gctacgggac cgacgatttc acgggggtcg aggccgcgaa gaaggccgcg
     1921 aagaggggtc gcgtatacgt gatgaaccag gggtatgcac agagggggga cttcgaggac
     1981 gcgggcttcc aggtagtgcg tgtggacgag tacgggatca tctacgagct tgtgccccaa
     2041 ggcagagagc cctacaaagc cgacgactag cggccgtccg gtgcgacgcc ttccagctgg
     2101 ccgccctcgg cggcgtagca ttccgccgcc gtgcactgcc tgagcgcgtc gagctccgcc
     2161 tcgagccttt gcttgagctc aggcgaggct gtcgcgaact ggttgtgcag ctcgtaaggg
     2221 tctctcctca ggtcgtacag ctcgtgttcg cccgtcttgt agtctacgta aagatagttt
     2281 tgcgtcctca gtgccttcat ccagggcctg ccccactcct cgctcgactg ggcgctcgcc
     2341 gccgaggtct tccgccagtt gttgggcagc gggtcacccg tgagcagcgg cgctaccgta
     2401 ctctcgttga tgaacggggg ctgggccacc ccgctacgct ccgcgacgga ctcgatgatg
     2461 aagcgctgac gccagtcttc gaggggggtg gggtcttctg tgagtagagg ttgtatcgag
     2521 cgcccatcca cgaagtttgg cgtcctggcc cctgccaggt cggcgaaggt cggcgcgagg
     2581 tcgttggtca gcaccatttg atgcagggtc ctgccttctg gcacgccagg accgcgtacg
     2641 atgagcggta tcctgatgtc ctcttcataa gccgtccatt tccccgcccc gagcctgtgc
     2701 tggcccaggt gaaagccgtt gtccgaggtg aagaatatgt atgtgttgtc cagctcgccg
     2761 ctctcgtgta gcgcctttac gaggtctccc accatgtcgt caacggccag catcgactgc
     2821 aggcgcttgc ggtggagctc ctccatgtat ttcttctgct ccaggctcag aggagggttg
     2881 tcgcttaccc aaccgggctt atccgagacg tccatctcgt cgaatgatgg aggccgcggc
     2941 agcgagacac cgggcaaggc ttccacgtcg cggggggccg ggttggcggg ctggtgtggg
     3001 gccttcgtac caagccacat caggaagggc cggtcggccg tgaagaacgg cgggtcggct
     3061 ccggccgtgc gcgtgatgta gtcggaggcc ttgtccgaga gcacgtcggt gtcgtagtag
     3121 gactctggat cgtagctgac catgtggccg ttctcgttga gcatgtggct gaggtagttt
     3181 ccggtcaccg cgtaccactc gtcccaccct gggggtacgt aggtctcgtt gtagccattg
     3241 agatacttcc cgaagaaggc cgtcctgtat ccctgatcct tgacccaggt agccatcgtc
     3301 gagttctcat gaccgagaaa gcggaacttc tggaagccgc cgcgcggcgg ctcgttgctg
     3361 aggatctggt ggctgtgggt gtactggccg cgcaggatcg tggccctgct cgggcagcag
     3421 agtgagttgg tcacgaacgc gttgtcgaag gtcgttccct tatccatgag cagcgacttc
     3481 aggtttggca tgtgttgcag cgtatccgag ttcaagtcgt tcgccgcaag gtcgtccgtg
     3541 aggatgagga tcacgttcgg aggttgcggc acttcctgct cctggtcggg ctgtttcccc
     3601 tccgccgaag ggaagcatgc cgagagcgag caggcggcgg ccagcccgag gagcgcgtag
     3661 atccctatca aaagtattct cttcgcctga ttcactccgg ccaccacatc gccgaacggc
     3721 gctcgttaga tcggcgttcg gacatcctca ccttccaacc ccgagaggtc ctcgctactt
     3781 cgaaaccttg cgcatcccgg gtctatgttt cctccaggtt aagtgttata cattcccgcg
     3841 atgggcgagt ctacttcaaa aataccggcg cggcgtgatc tctgacctga tccaggccct
     3901 cccggctgcc ctactggtgg gagtcctgcc tggatggttc tggacgaggt gcctgctggc
     3961 ctcgggggac cacgccgagc gactcgcata cacgatcgcg ctctccatca cgctggtgcc
     4021 gaccgccgcg ctggcccaga cgtacctgtt cacgacaggc gtcacgttcg ctgtgaccgt
     4081 cgcctcggtg gcgctcgtat tcctgaccgg gctggccgcc tacctgctgc tcgggcctgc
     4141 caaggggccg gaggaacccg tcttgcgccc gccgtcacgg cccggacttc ccacgctcat
     4201 accgctcgtc gtggccctcg cgctcgccct tgtgatgttg cttggtattg tgccaggcga
     4261 atggttaatg atcccgatag cgatcctcgt gctcgccgcg gggatcgcct tcttgtggac
     4321 agggagagaa gctccgccct ctgaagagaa gagagtaccc gcggcggcgc tttacgcgat
     4381 gctatgcgtg gtgctcctgc tcgtgctcgc ccgcggctac ctcggtcccg tcataaacgg
     4441 ctggcccttt cccagaggcg tagaccgcta cgagcacgcg gtgatggccg gcatgatgat
     4501 gagagaaggg tcaaacgaat cgttcatgct gtatccgccc ggcttccacc tcctaaccgc
     4561 catgctctcg cgcctctgcg ggttcgagcc tctcgaggtc ttccctatcc ttgccccaac
     4621 gctcccgctg ctctgcgcgc tggcgtgtta tgcgctggcg aagcgactct ggggtcctga
     4681 gtacggggtg gccgccgcgc tcacctctgg attgctcctc ggcggcacgt acatgcactt
     4741 cgaggaggcc cgctacccga acttcatagg agagtatttc ctgatcgtgc tggccgtcgc
     4801 cgccctgatc tggctgtacg ccgcgccttc ggcacgcgga ggtctgttgc tcgcgctcct
     4861 cggctcttcg acggtgctct accaccagat agccggctac agcctcgccg tgctgctcgt
     4921 attcgtgtca gtactcttct tgccctacct gctcctgaga gaccacagga gaggggtcta
     4981 cctgctgctc tccctcgcgc tcctcggctt cttctcggtg ctctacgcct gggacaccta
     5041 cgacctgccg agcctgctgg caggattgct gggcgggtcg gagacgggga agggagggga
     5101 ggccgtcgcg atggcgatag gaacaaagcc ggggaacggc cctggacact tcctgatgac
     5161 gatgtcacac cccgttctct ggctcggcct gcttggcctg ccgctaatgc tcgccggccc
     5221 caataatgga gaggataagt ctgccaccct ggcgcgcctc accctgctcg tttggaccct
     5281 gctgctgttc ttcggcagcc taacctccta cagtggattc cccgaccgtt tcgagcgcga
     5341 cctcggcgtg ccgctggcgc tgctcggggc actggcaatc gtgaccttgc tgcggtcacg
     5401 gtggtcgcca catcggacgg tcttcggggc gggcctgctc gcggtcgtcc tcacggcgac
     5461 cctcgtgggg gcgcaatcta tcctgaacct ggaggaagct gcgggagcga gcacacgcct
     5521 caaagacagg ccgcctccgg gcgaggtcgt cgccgccggc acgtggctcg aggagcataa
     5581 cgaaggcggc agcatagtcg cgactcctta cctggaatac gtcccgagcc gcgccatgct
     5641 cgcgatgggc ggatacacaa ggatgcaatc ctacgacgcg gccaggatac tgcgagccag
     5701 ggatcttccg cctttcggcg caggccctat gtgggaggcg ttgtggatcc tcaagcatcc
     5761 ggctggagag catacggatc aactcctgca gaaatacgac gtcaggtacg tcgttttcca
     5821 caaacgctat cctggcatcg actggcggcc cttcgcgtcc cggacaggcc tctaccggac
     5881 ggcattcgag aaccagggcg tcatcatctt cgcgccgcgt gaggattagc acctcaagga
     5941 agacgcgggc cgggtggcgg ttcgggaaga ccttgttaag ctatccggcg tgggttggcc
     6001 aaagtacacg aggagtgtga ggcgtggtac ttgacctgat ccgggccctg ctcgcggcgc
     6061 tagtcgtggc tgtcgctccc gggtggttct gggccgggct gctgcgaact tcaggggact
     6121 acgccgagcg cctcacctat tctgcggcgc tttcgatggc gctggtgccg gcaacggcgc
     6181 tgataccgac acggcttctg gggttgagcc ttacgcttcc aacagccctg gcctgcgccc
     6241 tcgtcgtgtt cctggcgggg ctcgggactt atctccggtt cggtgcggcc aaggggtcag
     6301 acgagcccat cttcccggcg cctgtcgcca ccctcagcgt gcccgcgctg gcgctgctcg
     6361 tgccggcgtt cggcctcgct tttggggtaa tgatcggcgc tatcccaggc gtgcccgtcg
     6421 cgatcccctt cacgagcgcc gtcgatcccg gcccacgggt aatggcaacg gtagccctcc
     6481 tggtgtgcgc ggcgggtaca gtccacctct ttatgtccag gcgaacggac ggatctgagg
     6541 cggtggaatc ccccgtgttg ccaggaccga tcttgcgccg gctcgcgctc cccgccgtat
     6601 tgttgctggc gatcgcgcgc ggctacctcg gacccgtgct gcacgactgg cccttcgtgc
     6661 gcggcgtaga ccattactcc cacgccgtga tggccgacag gatgatgagc gtggggagga
     6721 tagaacctta cctcatctac cctcccggct tccacaccct gacggcctcg atctcacgcc
     6781 tgagcggcct cgagcccctg gagatcttcc ccgtgctagg tcccgcagtg ctcctcctgc
     6841 caaccctcgc cctctacgcg ctggccaggc gtctgtgggg gtgggagtac ggggttggcg
     6901 cggctctgct ctctgtgctc atgggcggaa gctactacta cttcaacgac gcgatgtatc
     6961 cgaacctcgt tacctcccag ttccttctgg tccttgccct cgcatccctg gcaggcctct
     7021 acaaagcgcc ttccgcacgt aacggggtct tgttcgcgct gctcggttca tcggtcgtgc
     7081 tctaccatcc cgtcgcgagc atgtatctgg ccgtgcttct ggcggtggtc ggggcgtact
     7141 tcattgttcc cttgctattc cgcgaccgga ggacggggtt ggcgctgctg ctttcgtttg
     7201 cactcctcgg tttcctctcc gtcctgtacg cgtggaacac ctaccatctg ggacaggctc
     7261 tcgcgggact tgttgacagg tcgggcgaga gcaacacgac cgaagccgtc aagatggcgg
     7321 tgggcacaca ggcaccgtac gggatcgact tacagatcgg gaccatggtc tcccaacccg
     7381 tagcctggct cggcctcctc ggggttttcc tcctcgtggg tgacggaagg aggcgtatgg
     7441 gagtacctgc cgcgatagcg cacatcacgc tgttcctgtg gaccctgctg ctgttcgtcg
     7501 gcagccgcgc tgcgctgacc ggtttcccac aacgcttcgg acgcgacgtc ggcgtaccgc
     7561 tggccctgct cggagcactg gccctcgtaa ctatcttgag gtccctcggg acccgcaggc
     7621 gtgccgccgc ggtgttcgtc gcctccgtcg cggcgctcct ctgtgttgcg ctcgtgggcg
     7681 tgcagacggc gcagagcctc aaatttgcct ccggcccaag cttgcagaag acgatgacgc
     7741 cccagatcga ggcagccgga gcgtggctag aagagcacaa cacgggtgga aacatcatcg
     7801 tgagcccgca cgtcaaccag gtgccgagcc gcatgatgct cgccatgggc cattactccg
     7861 cactccagtc gttcgagctg tggcaggtcg cgaatcccag ggacctcccc cccacgggac
     7921 ccaaaccgct gtgggacatc ctctggctga tgaagcatcc tgacaacgag aggacggaca
     7981 gggccctcag cgagtacgac gttcgctaca tcgtgctcta caagaacatg cctgacaggc
     8041 cgaccgaaga ctactggctg gacttcaaga gtcaccccaa actttacaga acggacttcg
     8101 agaacagcga cgtcctgatc gtcgagcgac gcgagtgaag gagacgtgat atagcccctc
     8161 cgcggcccgc cgccgcttcg caggaggtcg acgagaaccg tatgagcacc ggggtgggat
     8221 tcatcctggc ggtctacgcg gcgagccgta tgttctacct gatctccggc tccctcctcg
     8281 ccaggatcgt gccaacctcc agcttccagc gcgtcacttc cgacgtgccc tcggggagta
     8341 tgaacctctg gtcacactgg gacggggaac actacgtggc gctggccttg ggaggatacc
     8401 tcaacccccc ggacaacgtc tcccctgcct tcttcccgct gtacccgctg cttatgagat
     8461 cgttcgcaga actcttcggt ggtcctcttt ctcagggtgc tctctccgtg tggggtcctc
     8521 tactctccct actcttcctc cccttcgccc tctacttcat ctaccactta gcccttgatg
     8581 aatgggga
//
LOCUS       DATAIR010000566         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_315404, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000566 DATAIR010000000
VERSION     DATAIR010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315404"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..2392
                     /locus_tag="VHM69_13405"
     CDS             392..2392
                     /locus_tag="VHM69_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX2741436.1"
                     /translation="MQQGHIIIRATRHAGVAIAEPTDPSKRPGERLKRWLLEHRIEDV
                     PGPEAKEGRSEQHPWWQVICLTGVDYFSTLGYIPGIAALAAGVLSPIATLLIVLLTLF
                     GALPMYRRVAAESPHGQGSIAMLENLLSFWRGKLLILCLLGFVATGWLVTITLSAADA
                     TAHIVENPLTPDFLHDQHVVITLVLLATLGAVFLKGFKEAIGIAVAVVGAYLLLNLVV
                     VSVGLYECVVGPSFVVDWQSKLFANYGNPLTMVGVSLLVFPRLALGLSGFETGVSLMP
                     LVRGDRDDEPQRPAGRIRNTRKMLTVAAIVMSFYLIMTSFVTVVLIPAAEFQKGGSAN
                     GRALAYLAHDQLGEVFGTVYDLSTIAILWFAGASALAGLLNIVPRYLPRYGMAPEWAR
                     AIRPLVLVYIAISVTVTIVFAADVDAQAGAYATGVLAMMTSAAFAVSLSAWRRGSRLG
                     TLRFGAITIIFVYAFVANEVQRPDGIVIASFFILTIVLTSLVSRVWRTTELRTEKIEV
                     DETAQRFIDEASGGEEIHIVANRRQSGDARDYYLKEREQRQYTHIPSDVILFLEVEVG
                     DPSEFEDVLEVRGVEVNGYRVLRAESSTVPNAIAAFLLHLRDTTGKETHCHFDWTQGN
                     PIGYLLQYVLLGEGDTAPVTHEVLREAERDPERRPIIHVAGK"
     gene            complement(2485..2757)
                     /locus_tag="VHM69_13410"
     CDS             complement(2485..2757)
                     /locus_tag="VHM69_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2741437.1"
                     /translation="MRRRIRALEPQLRALQRAFARELACPSAVYRVMDTTLVPAMVRV
                     RASRRGPGSPTCSRGTGWGGIRRALLGSVSESVIKHAHCPVLVVRE"
     gene            3120..3770
                     /locus_tag="VHM69_13415"
     CDS             3120..3770
                     /locus_tag="VHM69_13415"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2741438.1"
                     /translation="MAEENVIVVSFEEEAKAYQAASVLRQADADGRIDVHAVAIVQRM
                     EDGTLRVKEGEADDFPAATWTATALGAATGGIIGLTLGVLGGPLGVLLGGTYGMLLGS
                     LVDLSAVEEEESVLATMARAIQPGTTAVIAEVTEPTPAVVDEEMENLGGNVLRRPVAE
                     VEAELVAAADAARAAEEEARRRLVSEKHNEEQKENGEQKGKVGEKIERLKAKLAGR"
     gene            3896..>4842
                     /locus_tag="VHM69_13420"
     CDS             3896..>4842
                     /locus_tag="VHM69_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2741439.1"
                     /translation="MKREKDRAEHSYLGVWDPAGGEIREGSGLPVEEGQEEIRIVTRL
                     LRGRGKNHEISREELKGAVEDPDLLVWIDVLYPREGAAALLRDSLELGALTVEDCMEP
                     LRMPKMDVLSGDGSGGAQGAFVAAFAARLEDEARGPRLSASEVDLVTGPGYLVTLRDG
                     PVDEVERRLDVLMRSPEPGASGAALAHAAFDALVDGHLPVLVRASTIAEELEEVLDPR
                     NERASISALEALITLRRDLLAFRRLAVAQSEILRRLARISPEVSEYLSDVADNQREAV
                     DMADANRDYIEGAVEAYRVRRDERSDAGIRRLTILAGIIG"
BASE COUNT          950 a         1441 c         1552 g          899 t
ORIGIN      
        1 tcccgcgtgg ctttgcactc gcccgtaagc ggcgtcgcta tcccagcgcc ttgtccatac
       61 cctcggcggt gtgccttccc atgctgggca tacagtgcga gtagcggtcg aggttgcttg
      121 accctagtta gaccctaacc cggacgcaat accctgcaat agtgggcaat gcagggaaca
      181 gaaaaccctt tacttatgcg ggatttgcaa gtccgtgcaa cccccagcaa ccactgagcg
      241 cacactcgta aagagattag gtcagcggtt cgagtccgct cgtcggctct tttcgtgaag
      301 atgcgcaagg ttgtggataa cgtcatgcgt ttcgtacacc tctggtgtat agctaacctg
      361 atcaggcact tgttgcctca gttcaggtaa agtgcaacaa ggccacatta tcatcagggc
      421 aaccagacac gcgggagtag ccatagccga acctacggac ccatcaaagc gcccaggcga
      481 acgactcaag cgttggctcc tcgagcaccg catcgaggac gtaccaggcc cggaggcgaa
      541 agagggaaga tccgagcagc acccctggtg gcaggtgatc tgtctcaccg gcgtggacta
      601 cttctcgacg ctcggttaca tcccgggcat cgcggcgctc gcagccggag tgctctcgcc
      661 catagccacg ttgctcatcg tgctcctgac cctctttggg gccctgccaa tgtacaggcg
      721 cgtcgccgca gagagtcctc acgggcaagg ctcgatcgca atgctggaga accttctctc
      781 tttctggaga ggcaagctgc ttatcttgtg cctgctcggc ttcgtggcga ccggctggct
      841 ggtcacgatc accctctcgg ccgctgacgc cacggcccac atcgtggaga atcctttgac
      901 gccggatttc ctccacgacc agcacgtcgt catcacgctc gtgctgcttg cgacgctggg
      961 ggcggtcttc ctcaagggct tcaaggaagc catagggatc gcggtggccg tggtaggggc
     1021 gtacctgctg ctcaacttgg tcgtcgtgtc cgtaggactc tacgagtgcg tggtgggccc
     1081 atcctttgta gtcgattggc agtctaagct gttcgccaac tacggcaacc ccttgacgat
     1141 ggtcggcgta tcgttgctgg tgttcccgcg cctggcccta gggctttcgg ggttcgaaac
     1201 tggcgtaagc ttgatgccgt tggtgcgtgg cgaccgagac gacgaaccac agcggcctgc
     1261 aggccgcatc cgcaacacgc gcaagatgct caccgtggcg gccatcgtca tgagcttcta
     1321 cctcattatg acgagcttcg tgacggtcgt actcatcccg gcggcggagt tccagaaggg
     1381 aggaagcgcc aacgggcgcg ctcttgccta cctggcgcac gatcaactgg gtgaggtctt
     1441 cggcacagtc tacgacctga gcaccatcgc cattctttgg ttcgcaggag cctcggcgtt
     1501 agcggggctc ttgaacatcg tgccccggta tctgccccgc tacggcatgg cacccgagtg
     1561 ggcgcgagcc atccggccgc tggtgctcgt ctacatcgcc atctccgtta ccgtcaccat
     1621 cgtcttcgca gccgacgttg acgcacaggc cggggcctat gctaccggcg ttctggctat
     1681 gatgacctcc gcagcgtttg ccgtctcgct ctcggcctgg cgccggggtt ccaggctcgg
     1741 gacgcttagg ttcggagcga taaccatcat ctttgtctac gcgttcgtgg cgaacgaggt
     1801 gcagcgcccc gacggcatcg tgatcgcctc gttcttcatc ctcaccatcg tgctcacctc
     1861 gctcgtctct cgggtgtggc gcaccactga gctgcgcacg gagaagatag aagtggatga
     1921 gacggcccag aggtttatcg acgaagcgag cggaggagag gagatccaca tcgtcgccaa
     1981 tcggcgccag tcgggcgacg cgcgcgacta ttatttgaag gagagggagc agcgccagta
     2041 cacccacatc ccatcggacg tgatactttt cctggaagta gaggtgggag atccatccga
     2101 gttcgaggac gtgctcgagg taaggggcgt ggaagtaaac ggctacaggg tgctgcgcgc
     2161 cgagagctcg acggttccga acgctatcgc cgccttcctg ctccacctgc gcgacacgac
     2221 aggcaaagag acgcactgcc acttcgattg gacgcagggt aatcccattg ggtacctgtt
     2281 gcagtatgtc ttgctggggg agggggacac tgccccagtc acccacgaag tcctgaggga
     2341 agccgaacgt gaccccgaac ggcgccccat catccacgtc gccggcaagt gaccgagcag
     2401 gcgaccccca gcctactact ctggcccacc gttggatgag ggaaacgtca aatttcgatc
     2461 tcccctctta gcgctaatgt gagcctactc gcgtactact aggacagggc agtgggcgtg
     2521 cttgatcaca gattcggaga cgctgcccaa caatgctctt ctgatgccac cccagcccgt
     2581 gccgcgagag cacgtcggcg atccgggccc cctgcgagaa gccctcaccc tcaccatggc
     2641 cggaacgagg gtcgtgtcca tcactcgata gacggccgaa ggacaggcga gttctcgggc
     2701 gaaggcccgc tgcaaagcgc gcagctgggg ctccagggct cggatgcgtc gccgcatctt
     2761 gcaggataac cttttatgct gtggaggcta gcaagcaggc aagactcggg cgtcgtctga
     2821 atgactcgtt ttgtgctaag tcatattccc cacaccgcac gggcccgatc cgcagctcct
     2881 accgatgctg tacagcccga ttacccgatt gtcactgtag ggtagagaaa tttgcttacc
     2941 caaagatagg gagtcttgcc catgcttttg ctctgcgccc tgtttagact ccttggtccc
     3001 ggtgagaaaa agggtgagtt cggtccccag gcatactgag gcctgcgcgg cgcctctcag
     3061 gaagtaccag cgtgaagacg ggttactcgc tcgggaaaat gaaggaaagg ggtaggatca
     3121 tggccgaaga gaacgtcata gttgtgtcct tcgaggaaga agccaaagcg taccaggcgg
     3181 ccagcgtcct caggcaggcc gacgccgacg ggcgcatcga tgtgcacgcg gtcgccatcg
     3241 tgcagaggat ggaagacggc acgctcaggg tcaaagaagg cgaagccgac gatttccccg
     3301 ccgcaacctg gacagcgacg gccctcggcg ccgcaacagg tgggatcatc ggcctcaccc
     3361 ttggcgttct tggtggacca ctaggggttc tattgggagg cacctacggc atgctgcttg
     3421 gctctctggt cgacctcagc gccgtggagg aagaggagag cgtactcgcc acgatggcca
     3481 gggcgatcca gccaggcacc acagcggtga tcgctgaagt cacggagccg acgcccgcgg
     3541 tggtcgacga ggagatggag aaccttgggg gcaacgtact gcggcgtcct gtggcagagg
     3601 tagaggccga gctagtagca gcggcagatg ccgccagggc cgccgaggag gaagcgcgcc
     3661 ggaggctcgt cagcgagaag cacaatgaag agcagaagga gaatggagaa cagaagggga
     3721 aggtcggaga gaagattgaa aggctcaagg ccaagctagc tggcagataa ccaaggacgc
     3781 tgcttccagc atcttcgata ctggaggtga tgcaaccggc gcacgcctcg ctttggctat
     3841 cccccgattg cgaggccagg cagcgtaact agaactgccg ctgatcctat aatagatgaa
     3901 gcgcgaaaag gatagagcgg agcacagcta cctcggggtc tgggaccccg cgggcggcga
     3961 gatacgtgag ggaagcggac tcccggtcga ggagggccag gaagagatcc gcattgtgac
     4021 gcgcctactg cgtggcaggg gaaagaacca cgagatctcc agggaagagc tcaaaggagc
     4081 cgtggaggac ccggatctcc tggtgtggat agacgttctc tatccgcgag aaggtgccgc
     4141 ggctctgttg cgtgactccc tggagcttgg ggctctgacc gtcgaggact gcatggagcc
     4201 cctgaggatg cccaagatgg atgtcttgtc cggcgacggt tctggcgggg cacagggagc
     4261 gtttgtggcg gcgttcgccg cccgcctgga ggacgaagcc cgcggaccac gcctgagtgc
     4321 ctcagaggta gacctcgtga cgggtcccgg atacctggtt accctgcgcg acgggcccgt
     4381 ggatgaggtc gagaggcggc tggacgtcct gatgcgttct ccggaaccgg gggcctcggg
     4441 cgccgcacta gcgcacgctg ccttcgatgc cctcgtcgac ggccatctgc cggttttggt
     4501 acgggcttcg acgattgcag aggagctcga ggaggtgctg gatccccgaa acgagcgggc
     4561 ctctatttcg gcgctggagg cgctcattac cctgcggagg gacttgctgg cgttccgtcg
     4621 cctggcggtc gcccagagcg agatccttcg gcgcctggcg cgaatctccc cagaggtgag
     4681 cgagtacctc tcggatgtcg ccgacaatca gcgggaagcc gtggacatgg ccgacgcgaa
     4741 cagggactat atagaagggg ccgtggaggc ctaccgcgtg cgtcgcgacg aacggagcga
     4801 cgccgggata cggaggctca cgatcctcgc cgggatcatc gg
//
LOCUS       DATAIR010000567        11290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_407910, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000567 DATAIR010000000
VERSION     DATAIR010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407910"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VHM69_13425"
     CDS             complement(<1..851)
                     /locus_tag="VHM69_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2741440.1"
                     /translation="MSAPTKEQAAVIRLEHGPARVLAGAGSGKTHTMTELVSYRVDEH
                     LRKLGGTAPERILALTFTVKAADEMRSRLISRLGEQALKLTVANFHSYALQIVRENAA
                     VLGLETDAPVLRRGRAWLMILDELAARDLTLRRLDLSDPAGAADKVLTLLSQAKNDLV
                     DLGTLKSRTEEDLSRADTDGLVRVLDERLDLIELSQRFEARRAARGLLRYEDMIELGA
                     RVLEDPVMGETYRDRYEFVVVDEFQDTNPAQLRFVELLAGGDLSKVVVIGDDLQSIYN
                     FTGASIR"
     gene            complement(848..3793)
                     /locus_tag="VHM69_13430"
     CDS             complement(848..3793)
                     /locus_tag="VHM69_13430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2741441.1"
                     /translation="MASLDLNIDQLKAVEHPGGPLLVLAGPGTGKTGVLVARISHLVG
                     ERDVRPGRVLALTFSRRAADEMRARVTARLPEAEKVEVRTFHSFALSVVRRHAGSLGL
                     RSAPEIVPTNEQWALVSDILGTEDSESWGLPHGAFERPATVREVYDLMLRAQEHLHGP
                     KALRELGEETGRPYLVRAGAVLERYIDRLKWTTMTDYEGVVQYALRLLMPGGRTAGEI
                     SGLYDHVLVDEFQDTNRSQMELLKRLMPGESPNVFCVGDDAQSIYSFRGARIENVREF
                     GERFPGAREIHLRTNYRSAAHIVSLAEQAIAGDESRPRRDPQNVASIKKLGTVLHKVA
                     SSPREEGEWISDRIVELTQGQGVPREEIAILRRSLLDAAPLVDALASRGVPVDMAVSP
                     GGSSARHLATLLAASDGEEPDPTPAASALVSPLCGVAPEAARALRAASEASGRPVFGL
                     VRSGDSVVGIPDGEIEKARAVVQTVDAAAQEDSFAAKVDALWKGLPATKGLFESHAQE
                     QESALALTDALSFVRSAHAYARMSQRPSVDGFLRAGKMLHEDSDTWAPSAPPAEDAVR
                     LLTVHGSKGLEFEAVFVSGLVDERFPIRPRGVRFVDPGLLSSGTPTPRALLDQSHMFE
                     ERRLFYVALTRAKTYLFLTGVEERSEDGMKASPFLRELEDRLVELAESARPRRFWVSR
                     EEAIEELRRATCDQELPEPSRFAAARALAQMGERPLGEDGVDGWWRYLEQTEGSQAFP
                     ELGDLRARELLLHLDCPRRAFMERVSSGPARRGGTGRMAFGSVFGDGLRRFLEGDGES
                     LRDAVLASVEERDFGGPAFKEYWRRQALDAVASCEAWAHETRENLVTPNGEWEIEVGG
                     HKVHGRHGPLVEESGERVLVRVRTGKNPMKHAEAAVDPDLALTALGAEVDRAKYVYPR
                     KLSYGAPAERELDTSEGLEGFRAKAAAAVAEMELGEIPARPRSAEICGRCAFISICPL
                     HMEDEPWAG"
     gene            complement(3793..4746)
                     /locus_tag="VHM69_13435"
     CDS             complement(3793..4746)
                     /locus_tag="VHM69_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2741442.1"
                     /translation="MSTVKPEPLALEVALAHYVASLPEGKSVALEELAGELGCSVEEL
                     RRALESVIEVEDRDLTTISGVVIEDGRLVKYLHGGYGRDFRKPIRLSPVQGRAALLAL
                     DLVSIAVDPGILDELRGKVLAAVGREIPRVQAGAGAGDHEIAETIDQARREKRVLEVL
                     YPSGGKIKDRLVEPLLMSSIDGVWYLNAYCRYVDAPRLFRLDRIVSARLTDEHFEERE
                     GLELKTDFEDIDPRTYAARRAVVRFSPAIARWMEERPELDLIEVREDGSADYALYYTD
                     PGWAARRVMQYLGEAVVLEPEELRQEVHRQTSALLDRYESR"
     gene            complement(4743..5630)
                     /locus_tag="VHM69_13440"
     CDS             complement(4743..5630)
                     /locus_tag="VHM69_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2741443.1"
                     /translation="MRKQLHRDVDALAGAGITVQVESQEVRHYRLPSSGFSPVEVDLT
                     DEERAVLVGALRALRRDFPYAGPLRLALANLIGAASAGTLHGGHTDEAERVSLAAVAT
                     RDDEAVAGRVAFLESAVSRRKRVRFDYYSISRDEISTREVEPYTLSLLDGSWYLTGRD
                     LGREDLRQFRLSRIQGRILFVTKRDGGDFDVPESFERRLAGPRAPWQLGAPDQTACIR
                     VPRHVFDAARASYGEAVSLREDDGSSVLVTQYSGERQLAGWVVSLGEDAEILSPDSLV
                     DRTVEGLKRVVATHDRERA"
     gene            complement(5900..6820)
                     /locus_tag="VHM69_13445"
     CDS             complement(5900..6820)
                     /locus_tag="VHM69_13445"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX2741444.1"
                     /translation="MDHTLAKTLASLTIEEPVTHGPLHLFPLMGGTFTEEDLSLLEVA
                     IQEGTLRIEEMNESGSVPELRVLNEGTLPVLILEGDELVGAKQDRVVNSSVLVAADSE
                     LILPVSCVERGRWSYRSRAFSSGAGSPHLTLRHLKSRSVHDSLKHGRGHRSDQGAVWR
                     EVDRKARLHEATSPTHALQDSRSHLSEKLNAFEKLAEELPEGTRGVAVALGERPVLLE
                     VLAGPHSFARVSRKLLSGYAFEAVGPGNRGGNPDPSVVRDFIEAAAKARHDEHPAVGS
                     GRDVRFDEDGVSGYALVAQRGVLHAAAFAA"
     gene            complement(6996..7604)
                     /locus_tag="VHM69_13450"
     CDS             complement(6996..7604)
                     /locus_tag="VHM69_13450"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX2741445.1"
                     /translation="MTDQPPPATPNVDVQPPPPQSGRSWMRWLLIGGGGCLMLVVLML
                     LGFAGCLAVFSGGVDEASDDSKSTPKAEIGEPLRVGNVTWVVEDAKMVTELTSSYEKP
                     IQGNFVVVNFTFTNEGKEAATLDTASMALIDSEGRKSEADPDKFGYIPDDRNIFLENV
                     NPGVTDEGQAIFTVAPDSTGFKLELGDADLLTDDHGTVPLGF"
     gene            complement(7931..8149)
                     /locus_tag="VHM69_13455"
     CDS             complement(7931..8149)
                     /locus_tag="VHM69_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741446.1"
                     /translation="MPSTAPLWGVEHLLGPILEGKDLIIEGRLARTALERLGAEVEEL
                     RLGDEERYMDMTLAIFRRRKSPLREPRN"
     gene            8396..9076
                     /locus_tag="VHM69_13460"
     CDS             8396..9076
                     /locus_tag="VHM69_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741447.1"
                     /translation="MPTANIGAWIRIGVWALPAYALLTFWATLTHEPDRQTQVEAYAR
                     YISSSNYLTQHLLGSIFGTILAIFGVIALAAYLANSRSGALALVAMVLSVAGNCLILT
                     IFGFSTIVSPVIGQVYLAGRPGVMEVNEAIFGSAAFSLLAVSGLLVYLLGAILLGVAV
                     WFSGTLPKWAGALYAPTGFLIAAGVAIGAAQTLGSALLVVAGGWIAWSVMRGPSGQQG
                     PEARPRVR"
     gene            9418..9924
                     /locus_tag="VHM69_13465"
     CDS             9418..9924
                     /locus_tag="VHM69_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2741448.1"
                     /translation="MTVAARTVDLRTVALCLSYTGDMTIKRLDHISVVVDDLAAAIAF
                     FSELGLELEGEAAVEGEWVDRVNGLESVQVDIVMMRTPDGHGRLELTKFRNPELVEIE
                     PAIAPPNTLGLRSVMFTVESVDDTVARMRAHGAELVGEVAQYENLYRLCYMRGPGGII
                     VALAEELF"
     gene            complement(10936..>11290)
                     /locus_tag="VHM69_13470"
     CDS             complement(10936..>11290)
                     /locus_tag="VHM69_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2741449.1"
                     /translation="KPEVGLRIMQLLSERLRRQEMRLEDVGMKDVRARLASIKLLLVE
                     SEGVRSGTGYRIRSHYTHERLGTMIGANRVAVTRAFGRLQDEGVVQLRRRLIYVADIE
                     ALKRSATYGAAQEEE"
BASE COUNT         1947 a         3616 c         3498 g         2229 t
ORIGIN      
        1 ttacggatcg aggcgcccgt gaagttgtag atgctctgga ggtcgtcccc gatcacgacc
       61 accttcgaga ggtcgccgcc ggccaggagc tcgacgaagc ggagctgggc cgggttcgtg
      121 tcctggaact cgtctactac gacgaactcg tagcggtctc tgtacgtttc gcccattacg
      181 gggtcttcga ggacgcgggc gccgagttcg atcatgtctt cgtagcgcag gaggccgcgg
      241 gcggcgcgcc tggcctcgaa cctctgggac agctcgatca ggtccagacg ttcatccagg
      301 acacggacga gcccatccgt gtcagccctg gaaaggtctt cttccgtcct gctcttcagt
      361 gttccgaggt ccaccaggtc attcttggcc tgcgagagga gggtcaggac cttgtccgcg
      421 gcgccggcgg gatcggagag gtcgaggcgg cggagcgtga ggtctcgtgc cgcgagctcg
      481 tctaggatca tcaaccacgc cctgccgcgc ctgaggactg gagcgtcggt ctcgaggccg
      541 agcacggcgg cgttctcccg cactatctgg agtgcatagg agtggaagtt ggcgacggtc
      601 agcttgaggg cctgctcgcc gaggcgggag atcagacgcg agcgcatctc gtccgcggct
      661 ttgacggtga aggtgagagc caggatgcgc tccggcgccg tgcccccgag cttccggaga
      721 tgctcgtcta ccctgtaact cacgagctcg gtcatcgtgt gggtcttgcc cgagccggcc
      781 ccggccagga ccctcgcggg tccgtgttcg agccgtatca cggccgcctg ctcctttgtt
      841 ggtgcactca cccggcccac ggttcatcct ccatgtggag cgggcagatg ctgataaacg
      901 cgcagcgccc acaaatctcc gcactgcgcg gtctggcggg gatctctccc aactccatct
      961 ccgccacggc tgctgccgcc tttgcccgaa aaccctccag accttccgag gtgtcgagct
     1021 cgcgctcggc gggcgcgccg taggagagct ttctcgggta gacgtatttc gccctatcca
     1081 cctctgctcc gagggctgtc agggcgagat ccgggtcaac ggcagcctcc gcgtgcttca
     1141 ttggattttt ccccgtcctc acccgcacga gcacacgctc cccggactcc tcgaccaggg
     1201 gcccgtgccg cccgtgcacc ttgtgtccgc cgacctctat ctcccactct ccgttaggag
     1261 tgacgaggtt ctccctcgtt tcgtgtgccc acgcttcgca ggaagcgacg gcatcgaggg
     1321 cttgacgcct ccagtactct ttgaaggccg ggccgccgaa atcccgctcc tcgacgctcg
     1381 ccagcacggc atctctcagg ctctcgccat cgccttcgag gaagcgcctg agcccgtcgc
     1441 cgaagacgga gccgaaggcc atgcggccgg tcccgccacg tcgtgcaggg ccgctggaga
     1501 ccctctccat gaaagcccgc cggggacagt ccaggtgcag gagcagctcc ctggcgcgga
     1561 gatcgccgag ttccgggaac gcctgcgagc cctctgtctg ttccagatac cgccaccagc
     1621 catctactcc gtcctcgccg agtggccgct ctcccatctg cgcgagggct ctggccgcgg
     1681 cgaagcggga gggttcgggc agttcctggt cgcaggtagc acggcgcagc tcctcgatcg
     1741 cctcctcgcg ggagacccag aaccggcggg ggcgggcgct ctcggcgagc tccacgagac
     1801 ggtcctccag ctcgcgcagg aatggagagg ccttcatgcc gtcctcggat ctctcctcga
     1861 cgcccgtgag aaacaggtag gtcttcgcgc gggtgagggc gacgtagaac aggcgccgtt
     1921 cttcgaacat gtgactctgg tcgagcagtg cccttggcgt cggtgttccg gaggagagga
     1981 ggccggggtc cacgaacctc acgccgcgcg gacggatcgg gaaccgctcg tccaccagcc
     2041 cgctgacgaa gacggcctcg aactccaggc cctttgagcc gtggacggtg aggagacgga
     2101 ccgcatcctc cgccggcggg gccgagggcg cccaggtgtc cgagtcctcg tgcagcatct
     2161 tgccggctct caggaaccca tcgacgctgg gtctctggct catcctggcg taggcgtggg
     2221 cggagcggac gaaggagagg gcgtcagtca gggccagggc ggactcttgc tcctgagcgt
     2281 ggctttcgaa gagccctttc gttgcaggga gacccttcca cagtgcgtcc acttttgccg
     2341 cgaaagagtc ctcctgtgcg gcagcatcga ccgtctgcac gacagccctc gccttctcga
     2401 tctccccatc ggggataccg acgaccgagt caccggagcg aacgaggccg aagaccgggc
     2461 gcccgctcgc ctcagaggcg gcccgcaggg cgcgcgccgc ttccggggcg acgccgcaca
     2521 aaggtgagac cagcgcgctc gccgcgggcg tcggatccgg ctcctctcca tcgctcgcgg
     2581 ctagcagggt ggccaggtgt cgggctgagg agccacccgg agagaccgcc atgtcaacag
     2641 ggacgcctct cgaggcaagg gcgtcgacca ggggtgcggc gtcgagcagg ctgcggcgga
     2701 ggatggcgat ctcttcgcgt ggaacgccct gtccctgggt cagctcgacg atgcggtcgg
     2761 agatccactc tccctcctcc cgtgggctcg aagccacctt gtgcagcacg gtgccgagct
     2821 tctttatcga cgccacgttc tgcgggtccc tgcggggacg gctctcgtcg ccggcgatcg
     2881 cctgttccgc gagggagacg atgtgggcgg ccgagcggta gttcgtcctg agatgaattt
     2941 cgcgggcgcc ggggaacctc tccccgaact ccctgacgtt ctcgatcctg gcgccgcgga
     3001 agctgtagat gctctgcgcg tcgtccccga cacagaacac gttcgggctc tcccctggca
     3061 tgagcctctt caagagctcc atctggctgc ggttcgtgtc ctggaactcg tcgaccagga
     3121 cgtggtcgta gaggccggag atctcccccg ccgtccgccc accaggcatg aggagcctga
     3181 gggcgtactg cacgacaccc tcgtagtccg tcatagtcgt ccacttcaag cggtctatgt
     3241 atctctccag cacggctcca gcccgcacga ggtacggtct gccggtctct tcgccgagct
     3301 cgcgcagcgc ttttggtccg tgcaggtgct cctgggcgcg gagcatcagg tcgtagacct
     3361 cgcgcaccgt ggctggtcgc tcgaaggctc cgtgcggcag gccccatgat tctgagtcct
     3421 cagtgccgag tatgtccgag acgagcgccc attgctcgtt ggtcggaacg atctccggcg
     3481 cactgcgaag gcccagggaa cctgcgtggc ggcggacgac tgagagggcg aaggagtgga
     3541 aggtgcgcac ctccaccttt tcagcctcgg gcagcctggc cgttacgcgg gcgcgcatct
     3601 cgtctgcggc ccggcgggag aacgtgaggg cgagtacgcg cccgggcctg acatccctct
     3661 cccccacgag gtgcgagatc ctcgctacga gcacgccggt cttgcctgtg cccggaccgg
     3721 cgagcacgag cagcggtccg ccaggatgct ccacagcctt tagttggtcg atgttgagat
     3781 ccaggctcgc catcagcggc tctcgtacct atctagcaga gccgaggtct gacggtggac
     3841 ctcctggcga agctcctccg gctccagcac gacagcctcg ccaaggtact gcattaccct
     3901 cctcgcggcc cagcctgggt cggtgtagta gagggcgtag tcagcggagc catcttcgcg
     3961 cacttcgatc aaatccagct cgggacgttc ctccatccag cgggcgatcg ccggggagaa
     4021 gcgcaccacg gcgcgcctgg ccgcgtaggt gcgtggatcg atgtcctcga agtccgtctt
     4081 gagctcaagg ccctcgcgct cctcgaagtg ttcgtccgtc agcctggccg agacgatgcg
     4141 gtccaggcgg aagaggcgag gagcgtccac gtaacgacag taggcgttca ggtaccagac
     4201 tccgtcgatg ctggacatca agaggggttc gacgaggcgg tcctttatct tgccgcctga
     4261 ggggtagagg acctcgagca ctctcttctc ccgcctggcc tggtctatcg tctcggcgat
     4321 ctcgtggtct ccggctcctg cgccggcttg tacacgtggg atctcccgtc cgacagccgc
     4381 gagcaccttg ccacgcagct cgtccaggat gcccgggtcc acagctatgg agacgaggtc
     4441 cagtgcaagg agtgccgccc gtccctgtac gggagagaga cgtatgggct tgcggaagtc
     4501 cctcccgtag ccgccgtgca ggtacttgac cagccgtccg tcctcgatga cgaccccgga
     4561 aatggtcgtg agatccctgt cctcgacttc tatgacggac tcgagggccc tgcgcaactc
     4621 ctcgacggag cacccgagct cccccgcgag ctcttcgagg gccacgctct tgccttcggg
     4681 cagggaggcg acgtagtggg ctaacgctac ctcgagtgcc aggggttccg gcttgaccgt
     4741 gctcacgccc gctcgcgatc gtgcgtcgcc acgacgcgtt tgaggccctc tactgtcctg
     4801 tccacgagag agtctgggga caggatctcc gcgtcctccc cgagagaaac gacccaaccg
     4861 gcaagctgtc gctctccgga gtactgcgtg acgaggactg aagatccgtc gtcctcccgg
     4921 agcgacaccg cctccccgta ggatgctcgc gccgcgtcga agacgtgcct gggcactctg
     4981 atacacgccg tctggtcagg cgctccgagt tgccacgggg cgcgtggtcc tgccaggcgg
     5041 cgttcgaagg actccggcac gtcgaagtct ccgccgtcgc gcttcgtcac gaagaggatg
     5101 cggccctgga tccgggagag cctgaattgc ctcagatcct ccctaccgag gtcccgtccg
     5161 gtcaggtacc aggagccgtc gagcaacgag agcgtgtatg gttcgacctc cctcgtcgag
     5221 atctcgtccc gcgagatgga gtagtagtcg aagcgcaccc gtttgcgcct ggagacggcg
     5281 ctctcgagga aagctacgcg tccggccacc gcctcgtcgt ctctggtggc gacggcggcc
     5341 agggatacac gttctgcttc atcagtgtgt cccccgtgca gcgtacccgc ggacgccgcg
     5401 ccgatgaggt tggcgagggc caggcgcaag ggtcctgcgt acgggaagtc cctcctgagc
     5461 gcccgcaaag ccccgacgag gactgcgcgc tcttcgtccg tcagatctac ttcgaccggc
     5521 gagaagccgc ttgacgggag tctatagtgg cggacctcct gtgattcgac ctggaccgtt
     5581 atgcctgctc ccgcgagcgc atcgacgtcg cggtggagct gcttacgcaa ggcctcggca
     5641 gcatgttctt ggtcactgtg ctccgctagc gaggcgttgt ggatctcggc gtagggcggc
     5701 aggttctccc tgatggaggc ttcatccctg ggccggccat cgctagatag caggtaagct
     5761 gctaacgaga gctggcgtgc gaccgctttc tgattcctgt tcagaagtct tacccctcct
     5821 ccaggggatc aaaggacacc ttgtcgtccc caacctcagc aaggttatgt atatgatata
     5881 cgatgcaagc gttcgagttc tatgccgcga aggccgcagc gtgcagtaca ccacgctggg
     5941 ccacgagcgc gtagcccgag actccgtcct catcgaacct gacatcccgc ccagagccca
     6001 ccgcagggtg ttcgtcgtgc ctggctttcg ccgcagcctc gataaagtcc ctcacgactg
     6061 aagggtcggg attacctccg cggttgcccg ggccgacggc ttcgaatgcg tagccggaga
     6121 gcagttttcg ggagaccctg gcgaaggagt gaggaccggc aagcacctca agcagcaccg
     6181 ggcgctcgcc gagcgcgacg gccacgccgc gggttccctc gggaagctct tctgcaagct
     6241 tctcgaaggc gttgagcttc tcggagaggt gggaccgcga gtcctgcaag gcgtgtgttg
     6301 gcgatgtggc ttcgtgcagg cgggccttgc ggtcgacttc cctccaaaca gccccctggt
     6361 cgctcctgtg gccccggcca tgcttcaggg agtcgtgtac ggacctggac ttgaggtgac
     6421 gcagcgtgag gtgcgggctg ccggctccgg aggagaatgc tcgtgaacgg tagctccagc
     6481 gcccgcgctc gacgcacgag acgggcagga tgagttcaga gtcggctgca accaggacag
     6541 aagagttcac cacgcggtcc tgcttggcgc cgacgagctc atctccttcg aggatcaaga
     6601 ccggcaaggt tccctcgttt agtacgcgca attccggtac gcttccgctc tcgttcatct
     6661 cctctatacg cagcgtccct tcctggatag cgacttcgag gagtgagaga tcctcctccg
     6721 tgaacgtgcc ccccatgagg gggaacaggt gaagtgggcc gtgggttacg ggctcttcga
     6781 tcgtgaggct cgccagtgtc tttgccaggg tatggtccat tgtctggtcc tttctatcaa
     6841 ggtttcaggt cgtgctggcg gcattcggga agcccgtaca gatgtttaca gcagtctaag
     6901 aaggattcgc gccgtctggt ccaagtctgt gccctgcgag gccctgatac ccgaaacctg
     6961 tagcctgaag ccgcggtttg cgaagcaaat cgcaatcaga agcccagcgg tacagttccg
     7021 tggtcgtcgg tcaggaggtc cgcatcaccc aactccagtt tgaagcctgt cgagtctggg
     7081 gccacggtaa agatcgcctg accttcgtct gtcacgccgg gattgacgtt ctcgaggaag
     7141 atgtttcggt cgtcagggat gtagccgaac ttatcagggt cggcctcgct cttgcggccc
     7201 tcgctgtcga tcaatgccat cgaggccgtg tccagggtgg cggcttcttt gccttcgttt
     7261 gtgaacgtga agttgacgac cacgaaattg ccctgtatcg gcttctcgta actggaggtc
     7321 agttccgtga ccatcttggc gtcctcaacg acccaggtaa cgttacccac tctaagcggc
     7381 tcgccgatct cggccttcgg agtgctcttg gagtcgtcgg acgcctcatc gacgccgccg
     7441 ctgaagaccg ccaggcagcc cgcaaacccg aggagcatca atacgacgag catgagacat
     7501 ccaccaccgc ctataaggag ccaccgcatc cacgagcggc cgctctgggg tggtgggggc
     7561 tggacgtcaa cgtttggtgt cgccgggggt ggttgatcgg tcatgttcag cgcctttcgg
     7621 tttctggtgg tctggctttt cacactgtat ggggtgtggt ggacagaact tctggcagta
     7681 gcgaccagca tcatctccac gcgtcggtag tgatgcatac acgccgagta catatgcgtt
     7741 tacgacctga ggttgtggct actcgttttc cagtggaccc tgcagcacct gtatgatctc
     7801 gctggcagcg tacacccgat tgcgacgccg accgatgatc tcctccaaga cgcgcttttc
     7861 ttggagagaa ccgacatgac tgcgggctgc ctcgaaggat acgtttaaga cctccgccag
     7921 ctcgctgatg tcagttacgg ggttcgcgaa gaggtgactt acggcggcgg aatatcgcca
     7981 gcgtcatgtc catgtagcgc tcctcgtctc cgaggcgcag ttcctctact tcggcgccca
     8041 gcctctccag ggcagtgcga gcgaggcggc cttcgatgat caggtctttg ccttcgagga
     8101 tgggtccgag gaggtgttcg actccccata gaggagcggt agagggcacg catcaagctt
     8161 cgcggccttc tacctatcca tggaagagcg acggaggact agcgcgtatt tgacggtcac
     8221 tgtcagcaca tcggtaaata aaggtacggt gcgtctcctc cacgaccagg tccaaagggc
     8281 ctctgatcct ttttattcag tctcactacg aacttcgatg agaagtcttg tgcagcagac
     8341 actatcggct ggcatcatgc actgataacc gagcgcgcca gaaggaggtc gctctatgcc
     8401 gactgcgaac atcggggctt ggatccgcat tggcgtgtgg gcattgcccg catatgcttt
     8461 gttgaccttc tgggcgaccc tcacccacga gcccgaccgc caaacccaag tcgaggccta
     8521 cgcccgctac atcagctcct ctaactacct gacccaacac ctccttggca gcatattcgg
     8581 aaccatactc gccatattcg gcgtcatagc ccttgccgca tacctcgcga acagccgctc
     8641 aggagcattg gcgctcgtgg cgatggtctt gagtgtcgct ggcaactgcc tgatcctgac
     8701 catcttcggc ttctctacca tcgtctcgcc ggtgatcggg caggtgtacc tggcgggccg
     8761 gccaggtgtg atggaagtaa acgaggccat cttcggctct gcggcgttca gccttctcgc
     8821 cgtgtcgggg ctgcttgttt atctgttggg ggccatcctc cttggcgtag ccgtgtggtt
     8881 ctctggaacg ctgccgaaat gggccggagc tctctacgcc ccgaccggat tcttgatcgc
     8941 cgcgggggta gcgatcgggg cggcccagac cctcggaagc gcgttgcttg ttgtggccgg
     9001 gggatggatc gcctggagcg tgatgcgcgg accttcgggc cagcaaggac ccgaagcccg
     9061 gccaagggta cggtgagcca gcccagcgaa cgtcgtggac ccatcccaga gagccaagct
     9121 gtaatcgcca tcgaagacag cattccggcg tgaatgtgct cgagaccgcg gtggcgagta
     9181 cgccatccag ctagcgaacc tcatccatct actcgaggag gtattccagc gacgttcgga
     9241 gcgcatttag agatcctgac tctaaacccg atcctcgcaa gggttctcca ggatagagaa
     9301 catcccaaga agccctgtta caagcatcta agtacgccta acaagacccg cccaacccac
     9361 gtagcgtaag caatcgttaa gggacccacc tcttggaagc caggtggtcg gctgggtttg
     9421 acggtagcgg caagaacagt cgaccttagg accgtcgcgc tctgcctctc ctatactggg
     9481 gacatgacga tcaagagact tgaccacata agcgtcgtcg tcgacgacct tgcagccgct
     9541 atcgccttct tttccgagct cggtttggaa ctggagggtg aggcggcggt agagggagag
     9601 tgggtggacc gcgtcaacgg gctcgaaagt gtccaagtcg acatcgttat gatgcggacc
     9661 ccagatgggc acgggcggct cgagctgacg aagtttcgca atccagaact ggtcgagatc
     9721 gaaccggcga tcgcaccgcc gaacacgctg ggcctgagga gcgtcatgtt tacagtcgaa
     9781 agcgtcgacg acaccgtcgc tcgcatgcgc gcccacggcg ccgaactcgt cggcgaagtg
     9841 gcccaatacg agaacttgta ccggctctgc tacatgcggg gtccaggggg cattatcgta
     9901 gcgctggctg aagagctctt ctgaagcgtt caccgcttcg tgacggctcc gatcctccgc
     9961 ggaagcatac tgcttccgct ccaactcgcg ccgcggtttt gttgggcgca ctaagtgagt
    10021 gacgttggtc ctttggcgtc taacgtccga gaactgtgaa tgaggaccag cgactaccct
    10081 actggcgcca tgtctcctca aacgccagct tcgctttgag accatccttg ctcacgggac
    10141 caacccgcat tgtagtcagc agcccacgta ggaacctgcc tcaacgacat cgaccaggtg
    10201 cggaccgtgg ggaggacaga gtctgcggcc acgtgatggt catctctgac cctttcccgc
    10261 ttgccgatgt gcgcctcgtc tgaaagcatc tcggttcctc cgattccgct gattcaggat
    10321 cgcctactct actccaaacg agccacggcg cccatcgtgg cgccgtggcc cgcctttcct
    10381 actctttccc cgccatttac taggccacaa gggtatccga gagtctgtat cgtgtaacac
    10441 ttagtgtcgt ggcgcacgga ctcgatgcct cgcgtgcagt atatttggct tatcaccgag
    10501 ccggtcggag agagttcccc ctccatctct accggcccgg cgtcccgcat cagaggctgg
    10561 catacctcgc tcttatccgg tacaccttgc gccaaggccg gaggccgtta gtactctgat
    10621 tcgaataccg gtcgctttgc tcgaccgatc aagagccggc cgctcccacc ttcatgcagc
    10681 gcagcgacct ctgaggacct tagttcggcg agcatcggcg gatagggccg ctgattgact
    10741 gatgccagcc acactcaaat gtagattctt acgctcggga agataatatg tcacaatagt
    10801 tacgtatctt cgactatctt cctctcagtc aacaaggcag gcgttgcgcg gacggtgggg
    10861 agtcaatgca cccgacacgt aggtcattac cgtgacgcac ggagtcaagt cggttcgggt
    10921 gacggtagga ctccgctact cctcctcttg agcagcgcca taagtggctg atctcttgag
    10981 ggcctcgatg tctgcaacgt agataagacg ccgcctcaac tgcaccactc cctcgtcctg
    11041 gagccgcccg aacgccctgg tgacagctac cctgttggca ccgatcatgg tccctagacg
    11101 ctcgtgggtg tagtggcttc gtatcctgta tccagtgccg ctcctgacgc cctcgctctc
    11161 gaccaggagc aattttatgc ttgcaaggcg tgctcttacg tccttcatgc caacgtcctc
    11221 gaggcgcatc tcctgtcgcc tgagacgctc gctcaagagc tgcattatcc gcaatccaac
    11281 ctcgggcttc
//
LOCUS       DATAIR010000568         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_131218, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000568 DATAIR010000000
VERSION     DATAIR010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131218"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VHM69_13475"
     CDS             <1..389
                     /locus_tag="VHM69_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741450.1"
                     /translation="EIDVVLNITQVIAADGTVSFQQADPVSISLIQIGLIPTFYQFSE
                     ATIEVTMDIKTTTSRETNVKVSASAKVGFAAWSASVRADVSHNRKFGKEVQGTSRLFT
                     RLAPVPPPPRIAPELTVVDNRPDGGV"
     gene            448..942
                     /locus_tag="VHM69_13480"
     CDS             448..942
                     /locus_tag="VHM69_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741451.1"
                     /translation="MAEDTIRVDLLLRGVGQSMLEAQTQLNTAALEQPPGPEGLRTGV
                     AISETEIDVKMVFEDDGSGINVRPVTAGAARLSELDPGVLSGIRARLVAVADEEARPP
                     SRSPADVRQEVLSSPDIIRLQEIFGELQVETTYVGAVQRWIVDVKEPGGAILRSIQVP
                     DATQ"
     gene            971..1489
                     /locus_tag="VHM69_13485"
     CDS             971..1489
                     /locus_tag="VHM69_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741452.1"
                     /translation="MAEEPPTSSAREQIEGLIRENRTLGETLATLQKELESLQNRLQE
                     AVQARQSAEREAEQITQSNKQLQSTVAGLQNSKEQLEAQVGRLSLQVESTPLQPLTTK
                     EASTLFNQTLQELGDVSGFEVRNAELTLKLATAKLGEEPVLVLPQPDAVDPATLHELT
                     FSFRNVGLSETS"
     gene            complement(1763..2203)
                     /gene="dtd"
                     /locus_tag="VHM69_13490"
     CDS             complement(1763..2203)
                     /gene="dtd"
                     /locus_tag="VHM69_13490"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2741453.1"
                     /translation="MRIVLQRVSEASVAVDGDCISGIGPGLLLLVGVARGDGESEADW
                     LADKVAGLRIMGDEAGKMNRSVRDVGGAVLAVSQFTLLADTRKGKRPSFVAAAQPEEA
                     ARLFDYFCGRLRAAGVEEVQTGRFGAMMDVSLVNDGPVTIVLER"
     gene            complement(2209..2595)
                     /locus_tag="VHM69_13495"
     CDS             complement(2209..2595)
                     /locus_tag="VHM69_13495"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX2741454.1"
                     /translation="MDPAFQRLFTVEEANALLPTLREILADVALHRDALREKAPNMEP
                     VLRAAIGNGGGRGGSDYGVEAYNLYLDIERIRELGVVLKDLDLGLLDFPHERDGRVVF
                     LCWHPPEERIGYWHDLDAGYAGRQPL"
     gene            complement(2607..3389)
                     /locus_tag="VHM69_13500"
     CDS             complement(2607..3389)
                     /locus_tag="VHM69_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2741455.1"
                     /translation="MGLREGRLKSLTQRINYAAPYMYAVISDIHGNLEALEAVLRDVP
                     EDVESVYCLGDVIGYGANPDECCDVVRSLGMPTISGNHDLAVTDLGTDLNWFNPTAAA
                     AVLWTRDHLSEENAEFLRTRPRMMRTDEALFVHGSIRDPDEYIINKISAQENLEILRR
                     EYPDVPICFFGHTHVKTVAPSPNGSMTGGHTLDLRSGGPYLVNPGSVGQPRDGDTFAS
                     YVIADGEHVTYRFVEYDIRKAQVKIRAAGLPDMLADRLAVGR"
     gene            3450..3522
                     /locus_tag="VHM69_13505"
     tRNA            3450..3522
                     /locus_tag="VHM69_13505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3483..3485,aa:Arg,seq:ccg)
     gene            complement(3764..>4825)
                     /locus_tag="VHM69_13510"
     CDS             complement(3764..>4825)
                     /locus_tag="VHM69_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX2741456.1"
                     /translation="LITAGVAGAVGIFFTWRGQRLAREAQDHNQKNTQEQLRLTRRSQ
                     EQSQKSTQAQLENAQEELRLTRQGQITERFTRAIDQLGSEKLEIRLGGIYALERISSE
                     SAEDYWPVMEVLTAYVRKHAPLPCSKDWLRRTGQEGTEGAPIQTNSEEGSSGESEPFK
                     VPASAPDLQAVIIVLRRRTRYFRHGEPESLDLHETNLSGARLSRANLSGANLSGAAFW
                     GAILSGAILSGANLQLANLSRADLSGARLWEACLSDANFAEANLSGADLSGANLQLAN
                     LSRADLRGANVSRANLSGTRLSSADLSGADLSSVFLRGERLLKGNFSGALTQAQIDEA
                     RGDKRTKLPDHLKRPASWT"
BASE COUNT          873 a         1476 c         1526 g          950 t
ORIGIN      
        1 cagagatcga tgtagtcttg aacatcaccc aggtgatagc cgctgacggc acggtcagct
       61 tccagcaggc agatcccgtc tccatctccc tcatccagat aggtctgatc cccaccttct
      121 accagttctc ggaagccacc atcgaggtaa cgatggacat caagacgacg acgagcaggg
      181 agacgaatgt aaaggttagt gcctccgcca aggtaggatt cgccgcgtgg agcgcgtctg
      241 tgcgggcgga tgtatcccac aaccgcaagt tcggtaaaga ggtgcaggga acgagccggc
      301 tgttcacgcg ccttgcgccg gtaccgccgc caccgaggat agcgccggag cttactgtgg
      361 tggacaaccg tcctgacggg ggtgtgtagt ggcgtttgac ctgagtggga agaacccgct
      421 agagccgacg cggggaggtg aagtctgatg gccgaagata cgatacgcgt cgatctcctc
      481 ctccgcggtg tcgggcagtc gatgctcgaa gcccagactc agctcaacac ggccgcgctg
      541 gaacaacctc ccggtcccga aggattgcga acgggtgtgg ccatcagcga gacggagatc
      601 gacgttaaaa tggtcttcga ggacgacggt tcagggatca acgtccggcc tgtgaccgcg
      661 ggggctgcgc ggctctccga gttagatccc ggggtactct ccggcatacg ggcccgcctg
      721 gtcgcggtcg cggacgaaga agcgcgccct ccctctcgaa gtcccgcgga cgtccgccag
      781 gaagtgctct ccagtcccga catcatccgt ctgcaggaga tcttcggcga gttgcaggtc
      841 gaaaccacct atgtcggtgc ggtacagcga tggatcgtgg acgtcaaaga gcctgggggt
      901 gctatccttc ggagtattca ggttccggac gctacccaat aagggcgaga gccgggttga
      961 ggaggagtga atggccgaag agccccccac cagcagcgcc agggaacaga tcgaaggttt
     1021 gatccgcgaa aaccgaaccc tcggcgaaac cctcgcaact ctgcagaaag agttggagtc
     1081 tctccagaac agactgcagg aggccgtgca agcaaggcaa agtgccgaga gggaagcgga
     1141 gcagatcacg cagagtaaca aacagctgca aagtacggta gccggactgc agaactccaa
     1201 ggaacagctc gaagctcagg tcgggcggtt gtcgttgcag gtcgagagta cccctttgca
     1261 gcccctgaca acgaaggaag cgtcaaccct ctttaaccag acgctccaag agctaggcga
     1321 cgtcagcggc ttcgaagtac gaaacgccga gctcaccctg aagctcgcaa cagccaagct
     1381 tggggaggag cccgtcctcg tgctcccgca gcccgacgcc gtagaccccg ccaccctgca
     1441 cgagttgacg tttagcttcc gaaatgtcgg tctgtccgaa acatcgtgag gagccccgaa
     1501 gcacgctaag cccttcgggc ttcgcatgtc agcacggcgc ttcgcgcctt tcagcacgct
     1561 tcgggccttc taggccctcg ctctcagctg tcagcttttc ctctgaaggc gtcgcctaca
     1621 ctcacgaagt gacctatcgg gaagtaggca cttctctcga atggcggttt cagtatccac
     1681 gttcttgctg atggctgaca gcgaagacgc cagcctgagc gtgctgagtg ccgccgaccg
     1741 gagggaggcg ttgtgctgac agctaccttt caaggactat cgttacgggg ccgtcgttga
     1801 ccaaggacac gtccatcatc gccccgaagc ggcctgtctg tacttcctct acgccagcgg
     1861 cgcggagccg cccgcagaag tagtcgaaga gccgcgcggc ctcctcgggc tgggccgccg
     1921 cgacgaagct cgggcgcttg cccttgcgcg tgtccgcgag cagggtgaac tgtgagacgg
     1981 ccaggacggc gcccccgacg tctctgacgc tgcggttcat ctttcccgct tcgtcgccca
     2041 tgatcctcag gccggctacc ttgtccgcca gccagtccgc ctcggactca ccgtctcccc
     2101 gcgcgacgcc gacgagaagg aggaggcccg ggccaatacc tgagatgcaa tctccgtcga
     2161 ccgctaccga cgcttcactg acgcgctgga ggactatacg catggtctct agaggggttg
     2221 gcggccagcg tatccggcat cgaggtcgtg ccagtagccg atgcgttcct cgggcgggtg
     2281 ccagcagagg aagacgacgc gtccatcccg ctcgtgcggg aagtcaagga ggcccaggtc
     2341 aaggtcctta agtacgacgc cgagctccct gatgcgttct atgtccaggt agaggttgta
     2401 cgcttcgacg ccgtagtcgg agccgcctct cccgccgccg ttcccgattg cggccctcag
     2461 gacgggctcc atgttcgggg ccttctcgcg caaggcgtcg cggtggagcg ccacgtcggc
     2521 aaggatctca cgcagcgttg ggagtagcgc gttcgcttct tcgacggtaa agagtctctg
     2581 gaaggcgggg tccacgcctg ggtgattcag cggccgacgg ccagccggtc tgcgagcatg
     2641 tcggggaggc ccgctgcccg tatctttacc tgagctttcc gaatgtcgta ctcgacgaag
     2701 cggtacgtca cgtgctcgcc gtctgctatg acgtacgagg cgaaggtgtc cccgtcgcgc
     2761 ggctggccca ctgagccggg gttcacaagg taggggccgc cggagcggag gtccagggta
     2821 tgtccccccg tcatcgagcc gttcggagaa ggcgctacgg tcttcacgtg cgtgtgcccg
     2881 aaaaagcaga tcgggacgtc ggggtactcg cgcctcagga tctccaggtt ctcctgggcg
     2941 gagatcttgt tgatgatgta ctcgtcggga tcccggatgg agccgtggac gaagagcgcc
     3001 tcgtccgtcc gcatcatacg cggcctcgtg cgaaggaact cggcgttctc ctcgctcagg
     3061 tgatccctgg tccacagcac cgcggcagcg gccgtcgggt tgaaccagtt cagatcggtc
     3121 ccaaggtcgg ttacggcgag gtcgtggttg cccgagatcg tgggcatccc caacgagcgc
     3181 accacgtcgc agcactcgtc cgggtttgcg ccgtagccga tcacatcccc caggcaatag
     3241 acgctctcca cgtcctctgg cacgtcgcgc aggacggctt cgagcgcttc gaggttaccg
     3301 tggatgtccg agatcaccgc gtacatatag ggtgcggcgt agtttatacg ttgggtgagc
     3361 gatttcaatc gcccctctct caggcccacc ctccattgaa gcgccgcagg cgttaaggta
     3421 acatcatcgg gcggttcccg ctcttgcgcg ggcctgtagc tcaggggata gagcaacggt
     3481 ttccggggcc gtgtgtcgca ggttcgaatc ctgccaggcc cgctcctcgt ccgcgttatc
     3541 cctgattcgt cgtccacttt tggttggtgc cgctcgcatg ggtcgtggaa gaacccgcgg
     3601 gaactggtgg acatgaaggc cggagccgcg gggaagggag tcgcaccggg tgggacgtgc
     3661 cgggtgtgtg cggctatgag tctccttagc cgggattgat ctccatgtag acggctactg
     3721 gcggatttag gaatcatcgt tcgccggttt ggccggcggg cttctaggtc cacgacgcag
     3781 ggcgcttgag gtggtccggt agcttggtgc gcttgtctcc ccgcgcctcg tcaatctgtg
     3841 cttgagtcaa ggctcccgag aagttccctt tcaagaggcg ctctcccctg aggaacacac
     3901 tcgagagatc tgctccagag aggtctgcac ttgagaggcg cgttcccgag aggttcgctc
     3961 tcgagacgtt cgctccccta aggtctgctc tcgagagatt cgctagctgg aggttcgctc
     4021 ccgagaggtc tgctcctgag aggttcgctt ccgcaaagtt cgcatccgaa agacacgctt
     4081 cccagaggcg ggctcctgag aggtctgctc tcgagagatt cgctagctgg aggttcgctc
     4141 ccgaaaggat cgctcccgaa aggatcgctc cccagaaggc cgctcccgag aggttcgctc
     4201 ccgagaggtt cgctctcgag aggcgcgctc ccgagaggtt cgtctcgtgc aggtcgagcg
     4261 actcgggctc cccatgcctg aagtagcggg tgcgtcgccg gaggactatg ataaccgctt
     4321 ggaggtctgg tgctgaagcg ggaactttga aaggctcaga ctccccgctt gagccttcct
     4381 cggagttcgt ctgaatgggt gctccctcag tgccctcctg tcctgtcctg cgcaaccagt
     4441 ccttgctgca tggcaaggga gcgtgctttc ggacataggc ggtcaatacc tccataacgg
     4501 gccagtagtc ttccgcggat tcgctggaga tccgttcgag ggcatagatg cctcccagcc
     4561 ggatctccag cttctcgctc ccgagttggt cgatagcccg cgtgaagcgt tccgtgattt
     4621 gaccctgccg ggtgagccgc agttcctctt gggcgttttc tagttgtgct tgggtgctct
     4681 tctgactctg ctcttgggat cgtcgggtga gtctgagctg ctcctgagta ttcttctggt
     4741 tgtgatcttg tgcttcccgg gcaagcctct gccctctcca tgtgaagaag atgccgacgg
     4801 caccagccac ccccgccgtg atcaa
//
LOCUS       DATAIR010000569         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_38248, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000569 DATAIR010000000
VERSION     DATAIR010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38248"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VHM69_13515"
     CDS             complement(<1..385)
                     /locus_tag="VHM69_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2741457.1"
                     /translation="MNRQAAFLQKQKPLKEKYRGEPGSSRITLEARASQQDTPISCSV
                     GVGRAIYEAGAHAGVGGTGSAACSGDLLLGALAACAQITCQMVAEAMGIGTESIDVTV
                     EGDLDLAGTLGASRDVPVGFETIRTS"
     gene            680..1120
                     /locus_tag="VHM69_13520"
     CDS             680..1120
                     /locus_tag="VHM69_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741458.1"
                     /translation="MDDAIILFVDDKSRNADMFAQALRIFGYSNEVVKAGDGIEALDF
                     LFARGDHVGRDTGDEPRLILLDLNMPRMDGLETLRRLREDKRTKLLPVVMLTATDDSA
                     NKTEAYRLGINGYVDKLSDVPFPDMVKRIADYWLGLNEPATTDR"
     gene            1303..1542
                     /locus_tag="VHM69_13525"
     CDS             1303..1542
                     /locus_tag="VHM69_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741459.1"
                     /translation="MIQIRNVPDELHRTLKVRAAKAGMTLSDYLLSEIEHVAARPTME
                     EWLERVAKLEPVDVDEPPEVTIRRMRDADDPRDVE"
     gene            1549..1944
                     /locus_tag="VHM69_13530"
     CDS             1549..1944
                     /locus_tag="VHM69_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2741460.1"
                     /translation="MIVVDASAAVTMFLNLGPSAARVRERLGQVEVVHVPHLFDIEVL
                     HALRRHSLRGDLSGEQSWLIMDLLHEMNAVRYPHAPLAARIWELRENLTAYDAAYVAL
                     AEALDAPLVTMDARLAQAPGIRATVEVYG"
     gene            complement(1993..3258)
                     /gene="odhB"
                     /locus_tag="VHM69_13535"
     CDS             complement(1993..3258)
                     /gene="odhB"
                     /locus_tag="VHM69_13535"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX2741461.1"
                     /translation="MAAEIHVPELGESVADATVGRWLKQEGEAVKSGDALVELETDKI
                     NFEVEAEQDGVLESISKAEGETVNVGEVIGTIGEGDGAAPEEEPEAEAEEAEAEEVGG
                     EESNGHREVEDGGFRASPSVRKLAQEYEVDLAEVSGSGSGGRITREDVERLIRKQSQT
                     AAPEKTEEPERDGKGATQPAPAASEDGRAALEERVRVSRRRLTIAQRLVESQQTAAML
                     TTFNEADMSAVMALRKRRKESFQERTGARLGFMSFFTKAAVGALKAFPKVNAELQGSE
                     LILKKYYDIGVAVSTDEGLVVPVIRDADHKNFAGIEQGIADLATKAREGKLTLDDLRG
                     GTFTITNGGIFGSLVSTPILTLPQVTILGMHKIQDRPVVVDGEVVVRPMMYLALSYDH
                     RVIDGAEAVSFLVRIKELVEDPESLLLEG"
     gene            complement(3270..>5399)
                     /locus_tag="VHM69_13540"
     CDS             complement(3270..>5399)
                     /locus_tag="VHM69_13540"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX2741462.1"
                     /translation="MAHRGRLNVLAHVMDKPYSKIFGEFQQPEKGEQSSAAGRPGDAW
                     VGDVKYHLGVRGFQLGDEEADEEVFIHLAPNPSHLEHVNPVVEGMARAAQEERDEAGP
                     PRQDVDSSLPILLHGDAAFPGEGVSAETLNLSRLPGYRTGGTIHIITNNQLGFTTERQ
                     DARSTTYASDLAKGFEIPVVHVNADDPEACMAAARMAHAYREKFNKDFMIDLVGYRRF
                     GHNEGDEPAYTQPGMYETIRNHPTVREVWARELERRGIIEEAEAEAMFEEIMADMAEV
                     HKKPSDELTEEDFATDEMYTPLVQIPETTVAAEKLSELNEALLDIPEHFTPNSKLERL
                     FQKNRGDLDSIDWAHAETLAFATLLEDGTPVRLTGQDTERGTFSQRHAVFYDAETDER
                     YVPLQNLAQARASFSIYNSPLSEIAVLGFEYGYSVTSEDALVLWEAQYGDFANVGQPM
                     IDQFIVSGQSKWGQTSGLVLLLPHGYEGQGPEHSSARLERYLQLAAHENVRIGNLTTA
                     AQYFHLLRAQAALKDNKRPLIIMTPKSLLRHPMAASKLEDLAEGAFQPVLDDAEASAD
                     SVERLILCSGKIYTELVGSEYREEAVDTAVARIELLYPFPDERVREVLEGYPNLREII
                     WVQEEPQNMGAWTFVEPRLRELVGGELPILYVGKPPRPSPAQGSAKFHKQEHAGIVRE
                     AFENVGQNLDEEQAVEETQVRASSPSE"
BASE COUNT          911 a         1701 c         1724 g         1063 t
ORIGIN      
        1 agctggtgcg gatggtctcg aagccgacgg ggacgtctct cgaagcgccg agcgttccgg
       61 cgaggtccag gtccccttcc accgtgacgt cgatgctttc ggtccctatc cccatcgctt
      121 cggcgaccat ctggcaggtt atctgggcgc aggcggcgag ggccccgagc aggaggtcac
      181 cggagcaggc agccgagccc gtgcccccga ccccggcgtg tgctccggct tcgtatatcg
      241 cacgccccac gcccacggag caggagatcg gggtgtcctg ctggctcgct ctggcttcga
      301 gcgttatccg ggaagaaccc ggttcgccgc gatacttctc cttcaacggc ttctgtttct
      361 gcaaaaacgc ggcctgtctg ttcaaaccta ccccttcgct ctagtgcgcg actgacaaat
      421 gatacgcccg aagtgacgct attgcgtacc agtgcacctt agcacggctc tcctgcttga
      481 agagtggccg gagagcaaag aacgacacca gcttactaga cttcccgtac tgcgcctcag
      541 cccgacgctg atggagagtg tgcacttcac gaagccccag cggtcaggcc gccgggaaag
      601 tttagtaccg gcgacggcgg gtagcatacg aatagtggca tatacaggga caataacatt
      661 tgcttggggg tgagctgatg tggacgatgc gatcatcctg ttcgtggacg acaagtcccg
      721 gaacgctgac atgttcgcac aggctctgcg aatcttcggc tattccaacg aagtagtcaa
      781 ggcgggcgac ggcatcgaag ccctggactt cttgttcgcc aggggagatc acgttggccg
      841 tgataccggc gatgaaccac gtttgatcct gctggacctg aatatgccgc gcatggacgg
      901 actggaaacg ctgcggcgtc tgcgcgaaga caaacgaacg aagctgctgc cggtcgtgat
      961 gctaaccgca acggacgatt cagcaaacaa gacggaagcc taccgccttg ggattaatgg
     1021 gtacgtagac aagctttcgg acgtgccatt ccccgacatg gtaaagcgga tcgccgacta
     1081 ctggctgggt ctgaacgaac ccgccacgac agaccgatag ggcgggttag tacatctgaa
     1141 agcggctatt caacggcttc tgcacaggtc ggagatccaa gatcgtcctc ccgagtagtg
     1201 tcagaagcgt attgtagacg ttcgggtatg cctgaggatc gcacagcagg tccacagcat
     1261 cgcatgtgct gcgcatgtgt tactctcatt aacatgtcga agatgatcca gatccgcaac
     1321 gtcccagacg agcttcaccg cacgctgaag gtccgtgcgg cgaaggcagg gatgacgtta
     1381 tcagattacc tgctctccga gatagagcac gtggcggcaa ggccgacgat ggaagagtgg
     1441 ctcgaacggg tagcgaagct ggagcccgtc gatgtggacg agccgccgga ggttaccatc
     1501 cgcaggatgc gggacgccga cgatcctcgc gacgtggagt aacgtcgctt gatcgtcgtt
     1561 gacgcgtcgg cggctgtgac gatgtttctc aacctcgggc cgagtgccgc acgcgtgcgg
     1621 gagaggctcg gacaagtcga agtggtacac gtccctcacc tcttcgacat agaggtgctc
     1681 catgcactgc gacgccactc tctgcgcgga gacctgtcag gcgaacagag ctggctcatt
     1741 atggatctac tccacgaaat gaacgctgtc cgctatccgc acgccccgct cgccgctcgc
     1801 atctgggaac tgagagagaa cctgacggcg tacgacgcgg cttacgtcgc cctcgccgaa
     1861 gcgctggacg cccccctggt cacgatggat gcgcggctgg cgcaggctcc gggcatccgt
     1921 gccacggttg aagtctacgg gtagacgaag ggactccccg aagggagccc ctggcctggt
     1981 cgcatttctg gtctagcctt ccagcagcaa tgactcgggg tcttcgacca attccttgat
     2041 acggacaagg aagctcacgg cctcggcgcc gtctatcacg cggtggtcgt aggagagcgc
     2101 gaggtacatc atcggacgga cgacgacctc gccgtctacg accactgggc gatcctggat
     2161 cttgtgcatc ccgagtatcg tgacctgcgg cagggtgagg atgggcgtgg agacgagcga
     2221 gccgaagatg cctccgttgg tgatagtgaa cgtcccgcct cgcaggtcgt cgagggtcag
     2281 cttgccttcg cgggccttcg tagcgaggtc tgctattccc tgttcgatcc cggcgaagtt
     2341 cttgtggtcg gcgtcgcgga tgacggggac gacgagcccc tcgtccgtgc tcacggccac
     2401 gccaatgtcg tagtacttct tgaggatcag ctcgctcccc tgcaactcgg cgttgacctt
     2461 ggggaacgcc ttgagcgccc cgacggcggc cttggtgaag aagctcatga agccgagccg
     2521 cgcgcctgtg cgctcctgaa aggactcctt gcggcgcttc ctgagcgcca tgaccgcgct
     2581 catgtcagcc tcgttgaagg tggtgagcat cgcggcagtc tgctgcgact cgacgagccg
     2641 ctgggcgatg gtcaggcgcc gcctggagac gcgcaccctc tcctcaagcg ccgccctgcc
     2701 atcctccgag gcggcgggcg caggttgcgt agcgcctttg ccgtcgcgct caggttcttc
     2761 cgtcttctcg ggggcggcgg tctgggactg cttccggatc aaacgctcca cgtcttcacg
     2821 cgtaatgcgc ccgcccgaac cggaaccgga gacctcggcg aggtccacct cgtactcctg
     2881 ggccagcttc ctcacggaag gcgaggcgcg gaatccacca tcctcgacct cgcggtgccc
     2941 gttggattcc tcgccaccga cctcttcggc ctcagcctct tcggcctcag cttcaggctc
     3001 ctcctcgggg gctgcaccgt caccttcgcc tatggtgccg ataacctccc cgacgttaac
     3061 ggtctcaccc tcggccttag agatagactc gaggacgcca tcctgttctg cctcgacttc
     3121 gaagttgatc ttatccgtct cgagctcgac gagcgcatcg cccgacttca cagcttcgcc
     3181 ctcttgtttg agccacctac ccaccgtcgc gtcggcgacc gactccccaa gctcaggaac
     3241 gtgtatctca gcagccaatg ctcactctcc tattcgctcg gggaggaggc gcggacctgc
     3301 gtctcctcca cggcctgttc ttcatcaaga ttctgcccga cgttctcgaa ggcctccctc
     3361 acgatgccgg cgtgctcctg cttgtggaac ttggccgagc cctgggccgg gctggggcgc
     3421 gggggcttgc ccacgtagag gatgggcagc tcgccgccta cgagctcgcg cagccgcggc
     3481 tcgacgaacg tccaggcgcc catgttctgc ggctcctcct ggacccagat gatctcccgc
     3541 aggttcgggt agccttcgag cacttcgcgt acccgctcat cggggaaggg gtagagaagc
     3601 tctatcctgg cgacggcggt gtcaacggcc tcttccctgt actcgctccc gacgagctcg
     3661 gtgtagatct tgcccgagca caggatgaga cgctctacgg agtcggcact cgcctcggcg
     3721 tcgtcgagca cgggctggaa ggcgccttct gcaaggtcct ccagcttcga ggccgccatc
     3781 gggtgcctga ggaggctctt cggcgtcatg atgatgaggg gacgcttgtt gtccttgagc
     3841 gcggcctggg cgcgcagcag gtggaagtac tgggccgccg tggtgaggtt gccgatgcgt
     3901 acgttctcat gcgccgcgag ctggaggtag cgctccagcc tggcgctcga gtgctcgggt
     3961 ccctgcccct cgtagccatg gggcaggagc aggacgagcc ccgaggtctg gccccacttc
     4021 gactggccgc taacgatgaa ctggtcgatc atcggctgcc cgacgttggc gaagtctccg
     4081 tattgcgcct cccagaggac gagcgcgtcc tccgaggtga cagagtaacc gtactcgaag
     4141 ccgagaacgg ctatctctga gagcgggctg ttgtagatgg agaaggacgc tcttgcctgc
     4201 gccaggttct ggagcggtac gtagcgctcg tcggtctcgg cgtcgtagaa cacggcgtgg
     4261 cgctgggaga aggtcccgcg ctcggtgtcc tggccagtga gcctgaccgg cgtgccgtcc
     4321 tccaggagcg tggcgaaggc cagggtctcg gcgtgcgccc agtctatgga gtcaaggtcg
     4381 ccgcggttct tctggaagag gcgttcgagc ttggagttcg gggtgaagtg ctcggggata
     4441 tcgaggagcg cctcgttcag ctcggagagc ttctcggcgg cgacggtggt ctcggggatc
     4501 tggacgagcg gcgtgtacat ctcgtccgtg gcgaagtctt cctctgtgag ctcgtccgag
     4561 ggcttcttgt ggacctcggc catgtcggcc atgatctcct cgaacatcgc ctcggcctca
     4621 gcctcctcta tgatgccgcg gcgttcgagt tctcgcgccc acacctcgcg caccgtcggg
     4681 tggttgcgga tggtctcgta catcccgggc tgggtgtagg ccggctcgtc gccctcgttg
     4741 tggccgaagc ggcggtagcc gacgaggtcg atcatgaagt ccttgttgaa cttctcgcgg
     4801 taggcgtggg ccatcctggc cgccgccatg caagcttccg ggtcgtcggc gttgacgtgg
     4861 accaccggta tctcgaaccc cttggcaagg tcgctcgcgt acgtcgtcga tcgggcgtcc
     4921 tgccgctctg ttgtgaaccc cagctggttg ttggtgatga tgtggatggt gccgcctgtc
     4981 ctgtagccag gcagacggct caggttcagg gtctcggccg agactccctc gccagggaac
     5041 gccgcatccc cgtgcaagag gatgggcagt gaagagtcga cgtcctggcg cggcgggccg
     5101 gcttcgtccc gctcctcctg cgcagcgcgg gccatcccct cgacaacggg gttcacgtgc
     5161 tccaggtggc tcgggttcgg cgcgaggtgg ataaagacct cctcgtcggc ctcctcgtct
     5221 ccaagctgga agcccctgac gccgaggtgg tacttaacgt ccccgaccca cgcgtcgcca
     5281 ggtctgcccg cggcggagga ctgctcgccc ttctccggct gctggaactc gccgaagatc
     5341 ttcgagtaag gcttgtccat cacgtgcgcg aggacgttga ggcgtccgcg atgcgccat
//
LOCUS       DATAIR010000570         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_407993, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000570 DATAIR010000000
VERSION     DATAIR010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407993"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VHM69_13545"
     CDS             <1..589
                     /locus_tag="VHM69_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741463.1"
                     /translation="GGGLMEGRRERIVGGLAALSLVPGGLIAYMGYLWIRFGDPLLFY
                     SAQEYWNRRHTDPLTLFVDIVTEAYASLGDMFGAVPADSALGGVVDRIHGANDAYALL
                     FLLFTVALFALGFRVLPLSLSLYTILLLITAVSFGKPTTPLMGFSRYILVAFPLFITL
                     ATLLESRRAMVAWLSFSTVVSLVFCAFFVSWRFVA"
     gene            669..2069
                     /locus_tag="VHM69_13550"
     CDS             669..2069
                     /locus_tag="VHM69_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2741464.1"
                     /translation="MTIATINPATGEEVKTFEALTGDEIDRKLGLAAETFREYRKTSF
                     EERSRMLLRAAEILEEEAEDLGRLMTGEMGKTLAAATAEANKCARGCRYYAENAEKML
                     ADEAVELEGAKAFIRYQPIGPVLAVMPWNFPFWQVFRFAAPALMAGNVGLLKHASNVP
                     QCALAIEDIIHRAGFPEGAFQTLLISSSQVQAVIDDPRVRAATLTGSEGAGRQVASEA
                     GENIKKTVLELGGSDPFIVMPSADLDKAVNMAVISRTLNNGQSCINAKRIIVHEDIAD
                     DFESRYVRSMEALRVGDPMDEDTEMGPLATPDILEDVDGQVRKSVEAGARVLTGGEPI
                     EGPGNFYPPTVITDIPEHSPAYKEEIFGPVASLFRVRDIDEAIAIANDTTFGLGSSSW
                     TNDPEEQERFVNEIEAGMVYINRMTESTPEVPFGGVKNSGYGRELSAFGIREFVNAKT
                     VWIDASAEAATMAASE"
     gene            2183..2728
                     /locus_tag="VHM69_13555"
     CDS             2183..2728
                     /locus_tag="VHM69_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741465.1"
                     /translation="MILLRLVGIGAVYGGTFMFVKSLFPEPFVLLILQGGRSSEGDMT
                     TLALVYMGVGLLAGLIAGPIFGGALLLRRGRSEDPGPRLTLSLALALLTGFISGVLTL
                     IVYATGILPGGGVLDPLLLIGDANHPPGVPLLVAWTIARDLLPAGLAGLFLSPLVGGT
                     LQRLYAAERPPEQRRYDWEDI"
     gene            complement(2788..>3659)
                     /locus_tag="VHM69_13560"
     CDS             complement(2788..>3659)
                     /locus_tag="VHM69_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741466.1"
                     /translation="SPSNYNDGPENMPSPAPAEPYGTSLSVFDGTDPNGPWNLLIRDD
                     AGGDTGQITGGWSLDISIPPTDNDNFSNARAINTSTATINGDNLASSFETGEPNLLGG
                     GTVWHRWTAPNTGPTTIETCNTDYDSIMGIFTGSTVDSLTSPTPALGLINNGCLSGFG
                     DSTTFTAQAGTTYNIQVGGWPGGAPQGSFTLNLSGPANTPPTITPVNPLRPTRDRTPL
                     IRAAVTDTAGLTNSNIELRVDNRARTFSYDPATGALSYTSGKLSIGKHIVKLVATDAH
                     GGVTPKSWSFTIK"
BASE COUNT          660 a         1119 c         1168 g          712 t
ORIGIN      
        1 gggagggggg ctaatggaag ggaggaggga gaggatagta ggggggttgg cggcactttc
       61 attggtaccg ggagggctga ttgcatacat ggggtatcta tggataaggt ttggagatcc
      121 cctcctcttc tactcagcgc aggaatactg gaaccggagg catacggacc ccctgaccct
      181 gttcgtcgac atcgtcaccg aggcatacgc gagccttggc gatatgttcg gtgcggtccc
      241 tgcggattcg gcgctcggag gcgtagtcga tcgcattcac ggcgcgaacg acgcttatgc
      301 cctcctgttc ttgctcttca cggtcgcgct cttcgcgctc gggttcaggg tgcttccgct
      361 atccctcagc ctctatacaa tcctgctcct catcactgcc gtctccttcg ggaagccaac
      421 cacccccctg atgggtttct cgcgctacat cctggtcgcc ttccccctgt tcataacgct
      481 cgcgacgctc ctcgagagcc ggagggcgat ggtcgcctgg ctctccttca gtacagtagt
      541 ttcgctggtt ttctgcgcct tcttcgtcag ctggcgtttc gtggcgtgat cctgcaccgg
      601 ggtgtgaccg tcaccacccg ccgggtatat atttagcgat tggggacccg ttcgtaagga
      661 ggactatcgt gacgatagcg accatcaacc cggccacagg cgaggaagtg aagaccttcg
      721 aagccctgac cggcgatgag atagaccgga agctcgggct cgctgccgag accttccgcg
      781 aataccgtaa gacctcgttc gaggagcgct ccaggatgct cctcagggcg gccgagatcc
      841 tcgaggaaga ggcggaagac ctcggacgcc tgatgacggg cgagatgggc aagaccctcg
      901 ccgccgcgac cgcggaggcc aacaagtgcg cccgcggctg ccgctactac gcggagaacg
      961 ccgagaagat gctggccgac gaggcggtgg agttagaagg cgcgaaggcc tttatccgtt
     1021 accagccaat agggccggta ctcgccgtga tgccctggaa cttcccgttc tggcaggtct
     1081 tcaggttcgc cgcgcccgcg ctcatggcag gcaatgtggg tttgctcaag catgcctcaa
     1141 acgtgccgca gtgcgcccta gccatagagg acatcatcca cagagccggc ttccccgagg
     1201 gggctttcca gacgctcctg atctcctcct cacaggtcca ggccgtcatc gacgacccca
     1261 gggtgagggc ggcgaccctc accggcagcg aaggagctgg ccgccaggtc gcgagcgagg
     1321 ccggtgagaa cataaaaaag accgttctcg agctcggagg cagcgacccg ttcatcgtga
     1381 tgccgagcgc cgacctggat aaagccgtca acatggcagt catctcccgg acgctcaaca
     1441 acggccagtc ctgcatcaac gccaaacgaa tcatcgtgca cgaagacata gccgacgatt
     1501 tcgagagccg ctacgtcaga agcatggagg ccctgcgagt aggcgacccc atggacgagg
     1561 atacggaaat ggggccactc gccacccctg acatcctcga agacgttgac gggcaagtca
     1621 ggaagagcgt cgaggcaggg gcgcgcgtgc tcaccggcgg cgagcccata gagggtcccg
     1681 gcaacttcta tcctcccacg gtcatcacgg atatccccga acattcgccg gcttataagg
     1741 aggagatctt cggtcccgtg gcctcgcttt tccgtgtgag ggacattgac gaggcgatag
     1801 ccatcgccaa cgacaccacg ttcgggctgg gatctagctc ctggaccaac gaccctgaag
     1861 agcaggagcg cttcgtgaac gagatagagg cggggatggt ctacataaac aggatgacgg
     1921 aatcaactcc cgaggtccca ttcggcggcg tcaagaactc gggctacggc cgcgagctct
     1981 ccgccttcgg catccgcgag ttcgtcaacg cgaagaccgt ctggatcgac gcgagcgcgg
     2041 aagccgcgac catggcggca tcggagtagc gtgaaagctc ttgctgactg atggggcatc
     2101 accggctaag ctcgaaggtg tgatgagaga tggtggacat gcgcgagaag catctgccgc
     2161 cgggctgcac ggtgggtgga acgtgatcct tctgcgtctg gtgggtatcg gggccgtcta
     2221 cggggggact ttcatgttcg tcaagtcgct cttccccgag cccttcgtgc tcctgatcct
     2281 gcagggagga aggagcagcg agggcgacat gaccacgctc gccctcgtct acatgggcgt
     2341 cgggttgctc gcaggactca tcgcgggccc catattcggc ggcgctttgc tcctgcggcg
     2401 cggccgttct gaggaccccg gtcccaggct caccctcagc ctcgcgctcg cgctgctgac
     2461 cggcttcatc tccggcgtcc tcaccctcat cgtatacgcc acgggcatcc tccccggcgg
     2521 cggcgtcctc gaccccttgc tcctgatagg agatgccaac cacccgcccg gagtaccgct
     2581 cctcgtcgcc tggaccatcg ccagagacct gcttcctgcg ggcctcgccg gactcttcct
     2641 ctcgcccctg gtgggaggca cgctccaacg cctctacgcc gcggaaaggc cgccagaaca
     2701 gagacgctac gactgggaag acatctagca agtggataga gggccgggat gtagtgtccc
     2761 ggccctccgt agaaaacccg acctctgcta cttgatcgtg aagctccagc tcttgggtgt
     2821 tacgcccccg tgggcgtcag tcgccaccaa cttcacaatg tgtttgccga tggagagctt
     2881 gccgctcgtg taggagaggg cgccagtggc tggatcgtag gaaaaggtcc tggccctgtt
     2941 atcgacccgc aactcgatgt tgctgttcgt caaaccagcc gtgtccgtga ccgctgctct
     3001 tatgaggggg gtcctgtcgc gggtcggacg cagcggattc acgggcgtga tggttggcgg
     3061 agtgttagca ggacccgaga ggttgagggt gaagctcccc tgcggcgcac cgccgggcca
     3121 ccctccgacc tgtatgttgt aggtggttcc tgcctgggcg gtgaacgtcg tgctgtcgcc
     3181 aaaaccgctc aggcagccgt tgttgataag gcccagcgca ggcgtcggcg acgtgaggga
     3241 gtcgacggtg cttcctgtga agatgcccat tatcgagtcg tagtccgtgt tgcaagtctc
     3301 tatggtcgtc gggcccgtat tgggggccgt ccaacggtgc catacagtgc ccccaccgag
     3361 tagattgggt tcgccggtct cgaagctgct cgccaggttg tcgccgttta tagtggctgt
     3421 gctggtgttg atcgcccgcg cgttcgagaa gttgtcgttg tcggttggag ggatcgagat
     3481 atcaaggctc cagccgcccg tgatctgtcc cgtgtcgccc ccggcatcgt cgcgtatcaa
     3541 caggttccac ggcccgttcg ggtcggtgcc gtcgaacacg gagagcgagg ttccgtatgg
     3601 ctccgcgggt gccggggagg gcatattctc ggggccatcg ttgtagttgg aggggctta
//
LOCUS       DATAIR010000571         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_248288, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000571 DATAIR010000000
VERSION     DATAIR010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248288"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VHM69_13565"
     CDS             <1..681
                     /locus_tag="VHM69_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741467.1"
                     /translation="EVIVDEKTIARDMGFFLIFFAVAAGAGIVELPFAFRIALAAILI
                     SAYALYVRRTLHSGGAWEEVPQRLTLGRFSSRPPTWTVVCQAVLSLALIVVGAEFFVG
                     AVEGAAEVVALPAGLVALILAPLATELPEKINSVLWVREGKDTLALGNITGAMVFQST
                     VPVTLGVLFTPWELGPLNLFSAALAILSGSFVYAMLRRPASLHSWHLMVGGAFYLVFL
                     LVAVFAIF"
     gene            complement(688..1554)
                     /locus_tag="VHM69_13570"
     CDS             complement(688..1554)
                     /locus_tag="VHM69_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2741468.1"
                     /translation="MFPEKVLVSTDGSKDAARATEAASDLAVRSGAELHVVHVWHDVR
                     GFAHDFVKRELKRQGQEILDEQVERIRASGGDVAQAHLRMGRTSNEVLALSKEIDAGL
                     LVVGSRGLGTVQRILMGSQSEEIVHHAQIPVLVLRGGEEVWPPVRIVIGEDFSDDARK
                     AGELAAGLGKLYEAGGLLVYSHPDLPEVPPGEARSTVSDLMDIRGRDESMLDRRAAEL
                     EEILGSRPEVSVSEGYPANVLLEAAQEVQPSLVAVGSRGLAGIMRTRLGSVATKVVTA
                     ARGPVLVYPHVD"
     gene            complement(1640..>2227)
                     /locus_tag="VHM69_13575"
     CDS             complement(1640..>2227)
                     /locus_tag="VHM69_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2741469.1"
                     /translation="TEGEVRIDGRDVRELARKDLRANIGIVLQEPFLFSGTIAENIGY
                     GRSGDPSKATREEVEAAARVVDANDFITSLPEGYDTVLGEGGGALSQGQRQLLSFARA
                     ILTDPRILILDEATSNIDTRTEATIQKALATLLEGRTSVVIAHRLSTIRNADLILVID
                     DGRIAERGTHAELFDKNGLYADLYRRQFREPVASG"
BASE COUNT          316 a          765 c          691 g          455 t
ORIGIN      
        1 gaggtgatcg tggacgagaa aaccattgcc cgcgatatgg ggttcttcct gatcttcttc
       61 gccgtggccg cgggagccgg gatcgtcgag ctgcccttcg cgttccgcat cgccctggcc
      121 gcgatcctga tctccgccta cgccctttac gtgcggcgga ctctacattc tggcggcgcg
      181 tgggaggagg tgccgcagag actcacgctc gggcgcttct cttcgcggcc tccgacatgg
      241 accgtcgtct gccaggcggt cctttccctg gcgctcatcg tcgtcggcgc cgagttcttc
      301 gttggcgccg tcgagggcgc cgctgaggtc gtggcactac cggcgggtct ggtcgccctc
      361 atactcgccc ccctggcgac ggaactgccg gagaagatca actccgtcct ctgggtgcgc
      421 gaaggcaagg acaccctggc actcggcaac atcacgggcg ccatggtctt ccagagcacc
      481 gtacccgtca cccttggcgt cctgttcacg ccgtgggagc tcgggccgct gaacctcttc
      541 tcagcggcgc tcgccatcct ctctggtagc ttcgtctacg cgatgctacg ccgccccgca
      601 tcgctccact cctggcacct gatggtcggc ggcgcgttct acctcgtctt cttactggtg
      661 gccgtcttcg ccatattctg aacgcctcta gtccacgtgc gggtagacca ggacggggcc
      721 gcgcgcggcg gtcacgacct tggtcgccac gcttccgagc ctggtgcgca ttattcctgc
      781 caggccccgg ctgccgacgg ccacgagcga gggctgaacc tcctgggcgg cttcgaggag
      841 cacgttggca gggtagcctt cggagacgct gacctcgggc ctgctgccca ggatctcctc
      901 cagttcggcc gcccgccggt caagcatact ttcgtcccgc cctcgaatgt ccatgaggtc
      961 gctgaccgta gatctcgcct ctccgggagg aacctcgggg aggtcggggt gggagtacac
     1021 gagcaatccg ccggcttcgt agagtttgcc gaggcccgcg gcgagttctc cagctttgcg
     1081 cgcgtcgtcc gagaagtcct cgccgatgac gatcctgacc ggcggccaga cctcctctcc
     1141 gcctctcagc acgaggaccg ggatctgggc gtggtgcacg atctcctcgg actggctgcc
     1201 catgaggatg cgctgcaccg tgccgagccc gcggctgcct acgaccagta gcccagcgtc
     1261 gatctccttg ctgagggcga gtacctcgtt cgaggtgcgt cccattcgca ggtgcgcctg
     1321 cgcgacgtcg cctcctgagg ccctgatcct ctctacctgc tcgtcaagta tctcctgtcc
     1381 ctggcgcttg agctcccgct tgacgaagtc gtgggcgaag ccacgcacgt cgtgccacac
     1441 gtgaacgacg tgaagctcgg ccccgctcct cacggcgagg tccgaggccg cctctgtcgc
     1501 cctggcggcg tccttggatc cgtcagtgga caccagaacc ttctcgggaa acacgctcaa
     1561 cagggactcc tctctccttt aattactgaa acacctgcct gaatataaca gccagccgtc
     1621 cgttcgggtc cggcaagggt catcccgaag ccacgggttc gcggaactgg cggcggtaga
     1681 ggtcggcgta gagaccgttc ttatcgaaaa gctctgcgtg tgtcccacgc tccgcgatgc
     1741 ggccatcgtc tatgacgagg atgaggtccg cgttcctgat ggtgctcagg cggtgggcga
     1801 tgacgacgct cgtcctgccc tcgagcagcg tggcgagcgc cttctggatg gtcgcctccg
     1861 tgcgcgtgtc tatgttgcta gtggcctcgt ccaggatgag gatgcgcggg tccgtcagta
     1921 tcgcccttgc gaaggagagg agttggcgct ggccctgact caatgcccct ccgccctcac
     1981 ccagtacggt gtcgtagcct tctggcagcg aggtgatgaa gtcgttggcg tctaccacgc
     2041 gggccgcggc ctcgacctcc tctcgcgtcg cctttgacgg gtcgcccgag cggccgtatc
     2101 cgatgttctc cgcgatggtg cccgagaaca ggaaaggttc ctggagcacg atccctatgt
     2161 tcgccctcag gtccttgcgg gcgagctccc tcacgtctcg accgtcgatc ctgacctctc
     2221 cctctgt
//
LOCUS       DATAIR010000572        22372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_131288, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000572 DATAIR010000000
VERSION     DATAIR010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131288"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VHM69_13580"
     CDS             complement(<1..1009)
                     /locus_tag="VHM69_13580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2741470.1"
                     /translation="MKLAKRMERLGTETAFSVLAKAKALEAQGREIIHLEIGEPDFDT
                     PAHVVEAGCRALREGHTHYTPTAGIPELREAIAADVARSRGIEVGPEQVVVTPGGKPI
                     MFFAILALVDEGDEVLLPNPAFPIYESMVEFVGGRPIFVPLRQENGFRFDLDEFSAGL
                     SGRTKLVILNSPANPTGGVLTEEDIAGLASILRERPDVYVLSDEIYSRLLYTGNFASI
                     ASQHGLGSDERTIILDGFSKTYAMTGWRLGYGVMPEPLAEEVARLQVNSNSCTSAATQ
                     YAGIEALHGPQDAVQSMLAEFRARRDLIVSGLNELPGVECITPQGAFYAFPRISGTGH
                     SA"
     gene            1171..1293
                     /locus_tag="VHM69_13585"
     CDS             1171..1293
                     /locus_tag="VHM69_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741471.1"
                     /translation="MAGVEKQVKKAVKGKGAGGKKGKKGGSSKQVEKSAKKLLK"
     gene            1514..1720
                     /locus_tag="VHM69_13590"
     CDS             1514..1720
                     /locus_tag="VHM69_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741472.1"
                     /translation="MIRVRIEVDRGEASSTLSVRAESILRALEVAGEQNPDCALSVVF
                     PLDPDTFFVRDTSTVEHEVAEIAA"
     gene            1767..2876
                     /locus_tag="VHM69_13595"
     CDS             1767..2876
                     /locus_tag="VHM69_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX2741473.1"
                     /translation="MLTARADVVGSLLRPPELIEARERHAAGDLSAAEFKRIEDRAVD
                     WAIELQERAGLPVVTDGEMRRESFQSQMVEAVEGFGEHDIDAWLWGEWHGDETVGEKT
                     TERPPTLGVVGKLRRRRHLSVEEFVYARGRTERIVKVTLPSPSMYANLWSPELSTGAY
                     PTLDLFLEDVAEILREEVDELARLGAQYIQLDAPHYPLLLDQKTREFYERRGWDLDRW
                     LSRGIELDNHVIGAHGDVTFSFHLCRGNQGSRWLVSGSYEPLARHIFGGINAERLMLE
                     YDDERSGGFEPLAHVPEDKVAVLGLVTTKTPRRETPEELEARLREASRFVPLERLALS
                     PQCGFATSIVGNAISVEDEEYKLRTIVETAGRVWG"
     gene            complement(2914..3900)
                     /locus_tag="VHM69_13600"
     CDS             complement(2914..3900)
                     /locus_tag="VHM69_13600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX2741474.1"
                     /translation="MKAWQVQSLGDPEQALKLVEVDGPTPGPGEVVIEVEAAALNFFD
                     ILLCRGEYQERPDLPFTPGGEVSGTVNETGEGVGMQAGQRVIATPPLPRGGYSDKVAV
                     PVASVFPIPESMPFEKAAALPITYQTAHVALHHRARLEAGETVLVHAGAGGVGSAAIQ
                     LARAAGARVIATAGSPEKIVICERLGAEVSIDYREENFIEVVKEATEGRGADVVFDPV
                     GGDTFDGSRRCVAFEGRILVIGFAGGRIAQAPTNHVLVKNYSVVGVHWGLYARVMPEL
                     VRRTHDELVSLYEDGKLDPLIFETMPFERLPEALKLLGSRGTYGKIIIRPGD"
     gene            4020..4679
                     /locus_tag="VHM69_13605"
     CDS             4020..4679
                     /locus_tag="VHM69_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX2741475.1"
                     /translation="MLRVMDCMTKEVVTLGPEASAAEAWSLCREHNIRHVPVVQEGRL
                     AGLVSDRDLRDVRGGGGDRESDTPRWVRLDDMMTRNVVTIHPLDTIEHAARELYERKI
                     GCLPVVADDELIGIITSEDMMRTLIEMVGAQGRGTWVEVEVPNEPGTLAEVTDVIRDR
                     KVNVASIFLGPASRETNRLIVLRLETTNPAGIVATLRDAGYGVTTVESSFVAAKSPYE
                     E"
     gene            complement(4818..5729)
                     /locus_tag="VHM69_13610"
     CDS             complement(4818..5729)
                     /locus_tag="VHM69_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741476.1"
                     /translation="MRVLVTGARGKVGRATVAALQDAGHDVRATDLTPPVFERPEEGE
                     ADYVQADLTDAGDAFAVVRGAEAVVHTAAIPEPTSNPPHVVFQNNLMATFNTLEAAIR
                     FGVPRFVNISSETVPGFFFPERPFLPDYVPVDEEHPIRPQDPYATAKYFGELLMDAAL
                     RRSDIRCISIRPSWVQHEGNYERNLGPMVRDRSELSAGIWSYIDVYDLAYAIVLATES
                     DLSGHEVFYIASPDNVGGRPFEASVREHYGDQMEIKTPLSREDASGISIDKARRMLGY
                     DPKRSWRDYLDDEGRLKPGVKGLFSSS"
     gene            complement(5731..6180)
                     /locus_tag="VHM69_13615"
     CDS             complement(5731..6180)
                     /locus_tag="VHM69_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741477.1"
                     /translation="MELRDVNADNWRDVIRIEPREDQRRFVASVAYYLNLCHFGEVWR
                     PLALYMDDEPVGFAMWGFDTEEESYWIGGLVIDAEHQGKGYGRVAMEALLDHLAAQPG
                     YREAALSCDPENTVARRLYASLGFVETGERVDDELVARLQATQQRGG"
     gene            6293..7348
                     /locus_tag="VHM69_13620"
     CDS             6293..7348
                     /locus_tag="VHM69_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2741478.1"
                     /translation="MRIVVVGATGNLGTSVLRSLADEQEVESVLGLARRTPSLAMPKV
                     EWASVDVVNDDLTPHFRGADAVVLLAWIIQPSRDLNKQWMVNVEGSTRAARAVQEAGV
                     PSLLYASSVGAYSPGPKDRRVDEGWPTGGVPTSYYGRHKAEVERRLDRFEREAPGVRV
                     VRMRPGLVFKKEAAQGIRRLFAGPFFPGLLANPALINIVPEIEDLRSQVVHSYDVGEA
                     FRLAIVNEDARGAFNLAAEPVLDSREIARILDARTVPVPERLARAGAGLSWRLRMQPV
                     PPGWLDLSRQVPIMDTTRARTELGWTPRYSADFALLDLLEGLWTGAGLDTPPLARRTS
                     GPLRIREILTGVGGREP"
     gene            7345..8514
                     /locus_tag="VHM69_13625"
     CDS             7345..8514
                     /locus_tag="VHM69_13625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX2741479.1"
                     /translation="MKAVVWHGKEDVRVDNVPDPIIQESTDAIVRITSTAICGSDLHL
                     YAKLSPLMREGDIIGHEPMGIVEEVGSDVGHIAPGDRVVIPFNISCGHCYFCDQHLYS
                     QCEKTRDTGKLAQAVNLLGRGRGASLLGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPQ
                     GPPDERFLYLSDVLPTSWQAVVYADVPEGGTLGIWGLGPIGQMCARMALHQGAGRVIG
                     VDNVAERLSLASRWGVQTVDFSAVDNVRDVILDLTEGRGLDSVIDAVGMEASGSVVGS
                     ILQATKLQLDRATALRECMSAIRRGGTLSISGVYAGPIQAFPLGDLFDMQVQLRMGQA
                     NVKRWVDDLMPLLGDDDLLGTEDLKTHELPLDDAPLGYEMFQKKRDGAIKCVLKP"
     gene            8553..9509
                     /locus_tag="VHM69_13630"
     CDS             8553..9509
                     /locus_tag="VHM69_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2741480.1"
                     /translation="MAAGKKSGTEERGTKRSVYAAFTANLLIAVSKLIAGLISGSAAL
                     LAESVHSVADTINQVFLLVSLRSADEEPDDEHPFGHGKDRFFWSLLVAVGLFVAGSVF
                     SIYEGVSKILHGSGSHDAFFIGYVVLAAAFVFESGALLVSAREFRRTAHEANVSFREH
                     FRTTRNTTMKVPLYEDVAAIMGIAVAATGLFLTQITGNHVYDGLASIGIGVILVFVAW
                     ELGTDSRRLLIGEAMPPETRQKMEEIVLSFPEVVEVLRLLTMHLGPNSALLTAEINVV
                     DGLTTDEIEDLLECITQKVRSDVPEVTQTFIELHPEGSVGSY"
     gene            complement(9503..9991)
                     /gene="rraA"
                     /locus_tag="VHM69_13635"
     CDS             complement(9503..9991)
                     /gene="rraA"
                     /locus_tag="VHM69_13635"
                     /inference="COORDINATES: protein motif:HMM:NF006875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX2741481.1"
                     /translation="MPIKTADICDRFTESVEVCEPMFTSYGSVDGFDGWISTAKVYED
                     NVLVREALDDLAPGSVLVVDGGGSRRCALLGDKLASIAASRGLAGIVINGCVRDSREL
                     ADIEVGILALASHPLRSKKEGEGERDVPVEFGGVTWTPGHYVYADNDGIVLAAERLHQ
                     DQ"
     gene            complement(10003..11019)
                     /locus_tag="VHM69_13640"
     CDS             complement(10003..11019)
                     /locus_tag="VHM69_13640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2741482.1"
                     /translation="MKLDAGLGTEGKLKATDRTARAAEALGFAGLWTSETKHDAFLPL
                     AIAANATHEIELGTSVAIAFSRSPMETAQTAWDLQDLSDGRFVLGLGTQVKAHITRRF
                     SMPWDRPAARLREYILALRAIWESFRTEGPLQFDGEFYRHTLMSPFFNPGPIEHPEIP
                     VYIAGVNTRLARLAGEACDGFHVHPFHSPEYVRRTVKPAISEGAGEAGRDPEEVVLAT
                     SAFVIAAQNEEEAAEQRESVRAQISFYASTPTYRTVLEAHGWEEVGERLGTMAREKKW
                     REMPDLVTDEMLAAFAVEAAPDEIGGALKERYEGLIDRVALYLPFVPGERDEFWRAVV
                     ESAR"
     gene            complement(11028..11999)
                     /locus_tag="VHM69_13645"
     CDS             complement(11028..11999)
                     /locus_tag="VHM69_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX2741483.1"
                     /translation="MKAVRIHEFGGPEVLRYEDVEVSEPGPGQARVKLAASGVNYIDV
                     YQRKGLYPGDLPRTLGLEGAGEVEAVGEGVEDIAPGDYVAFASTPGSYAEYVVAPAQN
                     LVPFNVTLVEARVAAAVMLQGMTAHYLTHSTFPLQEGQTALVHAAAGGVGLLLVQLAK
                     LRGATVIGTAGTEEKAQLAREAGADEVILYTEQDFVEETNRITGGEGVHVVYDSVGKD
                     TFDGSLDVLRRRGYMVLFGASSGPVPPLDPQVLNHKGGLFLTRPSLAQYTTNREELLW
                     RAESLFSWIGQNNLSVRIGSTYSLADAEQAHRDLEGRKTTGKLLLIP"
     gene            complement(12150..13739)
                     /gene="pknB"
                     /locus_tag="VHM69_13650"
     CDS             complement(12150..13739)
                     /gene="pknB"
                     /locus_tag="VHM69_13650"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX2741484.1"
                     /translation="MRDVGKTVVDNRYDLVRPLGSGGMGEVYLARDRVLGRDVALKVL
                     RRQYAGDNEFAERFKREALSAASLSHPNIVQVYDRGETEEEASYIAMEYVPGGTLKER
                     ISEEGPLETADAAAIGAQVAEALGAAHDRGMVHRDIKPQNVLLTARGEAKVADFGIAR
                     AASSATISQTGSVMGTAGYMSPEQALGKPATPKSDLYSLGVVLYETLTGELPYTAENP
                     IAVSMKHVNEPLRPPRELNPRITEGMNALVTKLMAKDPDDRYASADELADDLWRVQRG
                     LPPVGADQMQGARTRPNAVAPEYFASKMPTARARTAPMRRRRGLPWLLLALVAVLFTL
                     GVLGGLQAVLGADAMNGWFDVFQAGKQPSPAQPSGPEKVKVPDVAGLTRSEAEQRLTD
                     AGLEVGQVGSFPSDTVAEGRVISAGREAGTPVEPGTEVNLTISSGPQPSSSASSSASA
                     SASAQSSAGASATAASSASAQPAQEEERQPAENQAAEEKAPKVKGPKVKVKAGPSLRG
                     PNLAAPARGLDEGKGHKHGKD"
     gene            13881..14120
                     /locus_tag="VHM69_13655"
     CDS             13881..14120
                     /locus_tag="VHM69_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741485.1"
                     /translation="MGRRGRGDSERPGRQEGGQGRQRDRSEGAADRHAARDGGRPEER
                     EDAGYKDRVHERLERMDARLERLEQRLEKLDGGGA"
     gene            14168..14689
                     /locus_tag="VHM69_13660"
     CDS             14168..14689
                     /locus_tag="VHM69_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741486.1"
                     /translation="MATSRVEENALRLLGAFYDLSGEMITEPVTIGGPEMPGQGAAPR
                     AGLDPESTECDVAARYLVNQGYIQSTGAASDYMITVAGFDRVREMRGIGDSAGERSGM
                     SDKTQKRLLTLLSIGISVGLSQPLTRFIGEEIPERRGIRDDVTEAVLKGLVRAVALVA
                     ASIIVRQLAASRR"
     gene            14739..15335
                     /locus_tag="VHM69_13665"
     CDS             14739..15335
                     /locus_tag="VHM69_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741487.1"
                     /translation="MDLASGAQILSSVAVLLLAVFAFVQVIEQRAARIAQDSPQILVD
                     VNYSQRTMINIVVRNIGRGAAKDIRFDFSAPVESSGGQRISDLAYFKEGIAFLAPNTE
                     IATVWDSYGDAVEVLKKKGLRNGITVTVKYKDLRGKAYITRWTINPLILEGSGYADYR
                     EFEDLVRALEKISRDIEGLREATVRLEAPREESDLDPR"
     gene            15404..16987
                     /gene="mdlC"
                     /locus_tag="VHM69_13670"
     CDS             15404..16987
                     /gene="mdlC"
                     /locus_tag="VHM69_13670"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HEX2741488.1"
                     /translation="MATVREATFDLLRRLGITTVFGNPGSTELPFLKDFPDDFRYVLG
                     LQEATVLGMAEGFAQGTGQAALVNLHTGPGLGNAMGAMITAWHNKTPLLVTAGQQDRR
                     HSALEPLLTGGLVELAKPYVKRSFEPVRSEDVPREILRAYHTAMQQPRGPVFVSIPMD
                     DWDSDAELLEDHDVVHRIAPDPDAIERFASILEEARSPAVVTGAGVDRAGAFYDAVTL
                     AEKLAAPVWQDPISPLASFPQDHPLFQGHLPPAQRQLAEQLSGHDVVLVLGAPVFLYY
                     PYVPGPTVADGTRVLHVSEDPEEAARAAVGTSVVGDVGLAIKGLVELLPDAYRPPPQS
                     KEPPPEPEAGTPMSVEYVMHTIAGVLPEEAVVFDETASSKAKLHMYIRINRPGGYHTS
                     ATGGLGFCMPGSVGFKLAVPERPVVCAVGDGSSMYSIQALWSAARYGAAVAFIVVNNR
                     GYSILKGFRDAIGAGDRVPGLDVEGIDFVGVAKGFGVEGELVEEAGDLLPAVKRALTA
                     GRPYLLEVVVDPEVPSLLS"
     gene            17000..17905
                     /gene="rbsK"
                     /locus_tag="VHM69_13675"
     CDS             17000..17905
                     /gene="rbsK"
                     /locus_tag="VHM69_13675"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX2741489.1"
                     /translation="MADVFVVGSINQDFVMYVDRRPEPGETVTDARLATHNGGKGANQ
                     AAAAALLGASVVFLGRVGDDGFGEPLVEALADKGVDASLIEVAPESSTGTAFITVTPD
                     GENAITVAPGANRRLTVENVDAASGSIGEAQVLVAQMEVPPEVVLRAVEVAARSGTRA
                     LVNLAPPFEVPRELLDKLDPIVVNEHEAAFLLGERVEGVDGALSAAPELLALGPKSAV
                     ITVGEAGAVFAEGKYTKHLPAPKVDVVDTTGAGDAFVGALAAQLARGSSLEDAVAYAV
                     RAGAAAVTKEGAQGALPTPETVESL"
     gene            complement(17937..18755)
                     /locus_tag="VHM69_13680"
     CDS             complement(17937..18755)
                     /locus_tag="VHM69_13680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741490.1"
                     /translation="MGLDLLSIVRLSDGGGLIYSGGIEEDGMVMAGKARSPVTSDTYE
                     VKNGYLDLLKTRAGAANVANLTNFIPGAGRGYEPLWRKHSLSLLTGESFPTERELEII
                     TDLVRPVRGARYLDLGCSSGLYTRSLARDLDDGAAVGIDISPSMLREAARRSRATGVK
                     PSFVRASAVNLPFIDDCFAGAVCGGSLNEFGDPARVLRETHRVLEPGGRLAMMGILRA
                     GTPRGRRLQRFLSTGGLKFFDPDELRSLLDHAGFEPDPVQTHGAVFFAGATSRT"
     gene            complement(18759..19394)
                     /locus_tag="VHM69_13685"
     CDS             complement(18759..19394)
                     /locus_tag="VHM69_13685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2741491.1"
                     /translation="MPPDGPSGVNEREYPWEVRDADADALRSIMSHYPTGVTVVTSGR
                     EEKVEGMTANAVISVSLDPLLFLVSVHKDARLNPRIREEGYFAVNLLADDQEGLSRLF
                     ASPERSSGLPALHSLGGGYGSTGAPLAAGALGVIECELAEIYAGGDHDLFLGRVVEVR
                     MGDTRKGPLVFHEGNYPTLNAVPRPEESGAFIDSVRGFDARLNRLRDRRRR"
     gene            complement(19433..20143)
                     /locus_tag="VHM69_13690"
     CDS             complement(19433..20143)
                     /locus_tag="VHM69_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741492.1"
                     /translation="MSSAPIVIVGGATWWPGSYRDFANMLREISGSEVHVAPVTPLDW
                     VLGYFRGFGQLVFEVASTVDRALLESEAKRAVLVGHSAGGLACRVYIGGVPPYGGRRY
                     SGHRRVERLITLGTPHNVADRERLSPITRVNELFPGALHDGLRYLSVAGSAVDGASSP
                     KARKRYERFVEDGCMAGDGVVPVDAAVLPGSESLVVDGVHHNRRLGLWYGSDRETVER
                     WWPEELRVGGSLVGDTRA"
     gene            complement(20164..20778)
                     /locus_tag="VHM69_13695"
     CDS             complement(20164..20778)
                     /locus_tag="VHM69_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX2741493.1"
                     /translation="MDFVFNAFVTLLVVVDPLGLAPIFAALTRGFSKKQKRESAIRGT
                     LLGALILYVFALAGDVLLEALGIGLPAFRIAGGLLLFLLSLDMVFARPSGMRATSVRE
                     QEEESYQQDISVFPLAIPLLAGPGAITTVLLYTGGSSTGQLLLFMGVLFVILVLTLIS
                     LLLAPRVMQLFGETGANVLSRVLGVLLAALAVQFVLDGLHKSLA"
     gene            complement(20802..21551)
                     /locus_tag="VHM69_13700"
     CDS             complement(20802..21551)
                     /locus_tag="VHM69_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741494.1"
                     /translation="MEILVAAEPYDDPEDGFGLQQAVLEEVAAGERGPVALMWTSSRY
                     VGATRQETRQAGFEAAARAASDLGFPVLVRNSGGGAVAANRGSLSFSLTFPVEDLRHG
                     LFERYSEGLDLIASALQQVGVDAERGEVDGEFCPGAYSVRSGGPWGVKHAGLAQRVTR
                     RAARLEALILVSETAEIRAVLGRFYEALGLPFRPGSVGDLPVDVARVIQALSEEVRNR
                     YVAAEGRIGAKTLERARSLRGEWRVAPETGT"
     gene            complement(21556..21753)
                     /locus_tag="VHM69_13705"
     CDS             complement(21556..21753)
                     /locus_tag="VHM69_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741495.1"
                     /translation="MTYSDPEYVRRQYRDPSNLNARIALHERFSTSFYGLTRWIFDQF
                     ELASRGAIHITKDVGTFVARK"
BASE COUNT         3967 a         7120 c         7310 g         3975 t
ORIGIN      
        1 ccgcgctgtg gcctgttccg ctgatacgcg ggaaggcgta gaacgcgccc tggggcgtta
       61 tgcactcgac gccagggagc tcgttgaggc cgctgacgat gaggtccctg cgggccctga
      121 actcggcgag catgctctgc acggcgtcct gcggaccgtg gagggcctcg atgccggcgt
      181 actgggtggc cgcgctggtg cacgagttgg agttgacctg tagcctggct acctcttctg
      241 caagtggctc gggcatcacg ccgtagccga gccgccagcc cgtcatcgcg tacgtcttcg
      301 agaagccgtc gaggatgatc gtccgttcgt cggagccgag cccgtgctgc gaggcgatgc
      361 ttgcgaagtt gcccgtgtaa agcagccggg agtagatctc atcgctcaag acgtagacgt
      421 cgggacgctc gcgcagtatg ctggcgagtc cggcgatatc ttcctcggtg aggacgcccc
      481 cagtggggtt ggcgggggag ttgaggatca ccagtttcgt gcgcccgctg aggcccgcgc
      541 tgaactcgtc gaggtcgaag cggaacccgt tctcctggcg cagtggtacg aagatgggcc
      601 tcccgccgac gaactcgacc atcgactcgt agatcgggaa cgcgggattg ggaagaagta
      661 cctcgtcccc ctcgtcgacg agcgcgagga tggcgaaaaa catgatcggc ttcccaccag
      721 gcgtgacgac gacctgttcg ggaccgactt ctattcccct gctacgcgcc acgtcagcgg
      781 cgatagcctc gcgcagctcg gggatgccgg ctgtgggggt gtagtgggtg tgcccttcac
      841 gcagcgcgcg gcagccggcc tcgaccacgt gggccggagt atcgaagtcc ggctcgccga
      901 tctcgaggtg gatgatctct cgtccttgtg cctctagcgc cttcgccttc gccaggaccg
      961 agaaggccgt ctctgtccca aggcgctcca tccgcttcgc cagcttcatc ccgctactcc
     1021 tccggtaaat gggattcatc acgcaccagc attacaccac acgcggtagt tcacaggcgc
     1081 ggagcgatgc ggccatacgg tttcgaggat gcctcgggag ggtagaggca tacatgtcag
     1141 gagtcgtcag gggaaggaga taactattct atggccggag tagagaaaca ggtcaaaaaa
     1201 gccgtcaaag gcaagggtgc cggaggcaag aagggcaaaa agggcgggag ctcgaagcag
     1261 gtcgagaagt ccgcgaagaa gttactgaag tagacaggcc atcatcgaat aacccgccgg
     1321 ggcttcgtgg ctccggcact tcttctgaac cttcctctcg ctgcgcagac ggatacgtat
     1381 ggggatctac ctgatccagg ttagatcctg gcgcgtattt ctgttgccta cattttgcgc
     1441 ttctgttacc ggcatgtaac agacgtgatg tacaaggagc ttgaagcatg acgctggcag
     1501 agggaggagc ggaatgatca gagtccgtat agaggtggat cgcggcgagg ccagttctac
     1561 gttgtccgtc cgagccgaga gcatcctgag ggcactcgag gtcgccggtg agcagaaccc
     1621 ggattgtgcc ttgagcgtag tgttcccact cgaccccgat acgttcttcg tgcgcgatac
     1681 gtctacggtg gagcacgagg tcgccgagat agccgcatag gcttgccggg acacgggctg
     1741 gtgctagact gcctctcgtg agcccaatgc ttacagcccg cgcagacgtc gtcggcagcc
     1801 tcctgcgccc gccggagctg atcgaggcgc gcgaacgcca cgcagccggt gatctctccg
     1861 ccgccgagtt caagcgcatc gaggacaggg ccgtggactg ggccatagaa ctgcaggaac
     1921 gggccggtct acccgtcgtc accgacggcg agatgcgcag ggagtccttc cagagccaga
     1981 tggtcgaggc cgtcgagggc ttcggcgagc acgacataga cgcctggttg tggggagagt
     2041 ggcacggcga cgagacggtg ggggagaaga cgaccgaacg tccgccgacc ctgggtgttg
     2101 tcgggaagtt gcggcggagg cgccacctct cggtcgagga gttcgtgtac gcgcggggcc
     2161 gcactgagag gatcgtgaag gtaacgctgc ccagcccgag catgtacgcc aacctctggt
     2221 cccccgagct atccaccggg gcctacccga cgctcgacct cttcctcgag gacgtggcgg
     2281 agatcctgcg cgaggaagtc gacgaactcg cgcggctcgg tgcacagtac atccagctcg
     2341 acgcgccgca ctatcctctc ctgctcgacc agaagacccg cgagttctac gagcggcggg
     2401 gctgggacct ggaccgctgg ttgagcagag ggatagagct ggacaaccac gtgatcgggg
     2461 cccacggcga cgtgacgttc tctttccacc tctgcagggg caaccagggc agcaggtggc
     2521 tcgtctcagg ctcgtacgag ccgctggcgc gccacatctt cggcggtatc aacgccgagc
     2581 ggctgatgct ggagtacgac gacgagcgct cggggggctt cgagccgctc gcccacgtgc
     2641 ccgaagataa agtcgccgtc ctcggcctcg tcacgacgaa gacgcccagg cgcgagacgc
     2701 cggaggagtt ggaagccaga ctcagggagg cttcgcggtt cgtgccgctg gagcggctgg
     2761 cactcagtcc gcaatgcggc ttcgccacgt ccattgtcgg gaacgccata agcgtcgagg
     2821 acgaagagta caagctgcgc accatcgtcg agaccgcggg gagggtctgg ggttagcgtt
     2881 caaggcatcg tccggtaaag accggagaac gggttaatcg cccggacgta tgatgatctt
     2941 gccgtaggtg ccccggctgc cgagcagctt cagggcctcc ggcaatctct cgaagggcat
     3001 tgtctcgaag atgagaggat ccaacttccc gtcttcgtag aggctaacca gctcgtcgtg
     3061 agtcctgcgg acaagctcag gcatgaccct cgcgtagagt ccccagtgca cacccactac
     3121 cgagtagttc ttcaccagga cgtgatttgt cggggcctgg gcgatcctcc ctcccgcgaa
     3181 accgatgacc aggatcctgc cttcgaaagc gacgcaccgc cgcgagccgt cgaaggtatc
     3241 cccgccgacg ggatcgaaga cgacatccgc gccccgcccc tccgtcgcct ccttgacgac
     3301 ctcgatgaag ttctcctccc tgtagtctat agagacctcc gcaccgagcc tctcgcagat
     3361 gacgatcttc tcagggctgc ctgctgtggc gatgactcgc gcccctgcgg ccctcgcgag
     3421 ctggatggcc gcggaaccga cgcctcccgc gccggcatgg acgagcacgg tctcgcccgc
     3481 ctccagtcgc gccctgtgat ggagggctac gtgggccgtc tggtaggtga tggggagcgc
     3541 cgcagccttc tcgaagggca tcgattccgg tatggggaag acgctcgcga cgggtacggc
     3601 gaccttgtct gaataaccgc cgcgcgggag agggggagtc gcgatgacgc gttggcccgc
     3661 ctgcataccg acgccttcgc cggtctcgtt gactgtcccc gagacctcgc cgccgggggt
     3721 gaaaggaagg tccggcctct cctggtactc gcccctgcac aggaggatgt cgaaaaagtt
     3781 gagcgccgcg gcctctacct cgatcacgac ctcgccgggc cctggcgttg gcccatcgac
     3841 ctcgacgagc ttcagcgcct gctccgggtc accgaggctt tgcacctgcc aggctttcat
     3901 agcggatcct ccctccagtt catgggcttc gagcctacca cagcgcctgt ccagccggga
     3961 tggtaagctc cgctgagaac gtagaaagag cggccgcaga agaggacagg agcgtctgta
     4021 tgcttagggt tatggactgc atgacgaaag aagtcgttac gctagggccg gaggcgagcg
     4081 ccgcggaggc gtggtccctt tgcagggagc acaacatccg tcacgtgccc gtcgtgcaag
     4141 aagggcggct cgcgggcctg gtctcggaca gggaccttag ggacgtcaga ggcggtgggg
     4201 gagaccgcga gtcagatacc ccgcggtggg tgcgactcga cgacatgatg acaaggaacg
     4261 ttgtcaccat acacccgctc gataccatcg aacacgctgc gcgcgaactt tacgagcgca
     4321 agataggctg tctccccgtc gtcgctgatg atgagctgat cggcatcata acctccgagg
     4381 acatgatgcg taccctaatt gagatggtcg gggcgcaagg ccgcgggacc tgggtcgagg
     4441 tcgaggtccc gaacgagcct ggcacgctcg ccgaggttac cgatgtgata cgggaccgca
     4501 aggtcaacgt agcgagcatc ttcctcggtc cggccagccg tgagaccaac cgcctcatcg
     4561 tgctgcggct ggagacgacg aacccggcag gaatagtggc gaccctgagg gacgccggct
     4621 acggcgtaac caccgtcgag tcttcgttcg tcgcggccaa gagtccttac gaggagtagc
     4681 tctttgagcg aagtgcagaa gtcaggactt ctgcacttcg cgctttcagc cttcagcttg
     4741 ttgctgcgaa ttttgttgat tccagaggtt cgttggctga gagcgtttgc aggttggatc
     4801 ttattgcaag ggcagcttta ggaactcgag aacaatccct ttacgccagg cttcaatcgt
     4861 ccctcgtcgt cgaggtagtc gcgccaggag cgtttggggt cgtagccgag catgcgcctg
     4921 gccttgtcta tggagatgcc tgaggcgtcc tctcgggaga ggggcgtctt tatctccatc
     4981 tggtccccgt agtgctcgcg cacggacgct tcgaacggcc tgccgccgac gttgtcgggc
     5041 gaggctatgt agaagacctc gtgtcccgac aggtcgctct ccgtggctag cactatggcg
     5101 taggccaggt cgtagacgtc gatgtagctc cagatgcccg cgctcagctc cgagcggtcg
     5161 cggaccatcg gaccaaggtt gcgctcgtag ttgccctcat gctgcaccca ggacgggcgg
     5221 atcgagatgc accggatgtc cgagcgcctg agagctgcgt ccataaggag ttcgccaaag
     5281 tatttggccg tagcgtaggg atcctgaggc cggatcgggt gctcttcgtc cacaggcacg
     5341 tagtcgggca agaaaggccg ctcagggaag aagaatcccg gcacggtctc gctcgaaata
     5401 ttcacgaaac ggggtacgcc gaaccttatc gcggcctcca gcgtgttgaa cgtcgccatg
     5461 aggttgttct ggaacaccac gtgcggcggg ttgcttgttg gctctgggat cgccgctgtg
     5521 tgtacgacgg cctccgcgcc ccgcacgacc gcgaaggcgt cgcccgcgtc ggtgaggtcg
     5581 gcctggacgt agtcggcctc gccctcctcg ggccgctcga agaccggcgg ggtcaggtcc
     5641 gtcgcccgca cgtcgtggcc tgcatcctgg agcgccgcaa ccgtcgccct tccgaccttt
     5701 cccctcgctc ccgtaaccag taccctcacg tcaacctcct cgttgttgag tcgcctggag
     5761 gcgcgcgacg agctcgtcgt caacccgctc gcccgtctcg acgaacccga ggctagcata
     5821 gagtcggcgc gcgacggtgt tctcgggatc acatgagagc gctgcctccc tgtatccagg
     5881 ctgcgcggcg agatggtcga gcagcgcctc catcgccacc cttccgtagc cttttccctg
     5941 gtgttcagcg tcgatgacga gcccgccgat ccagtagctt tcttcttcgg tgtcgaatcc
     6001 ccacatcgcg aatccgacgg gctcgtcgtc catgtagagt gccagaggac gccagacctc
     6061 cccgaagtgg cagaggttga ggtagtaggc cacgctcgcg acgaaccgcc tctgatcctc
     6121 gcgcggctcg atgcgtatca cgtcgcgcca gttgtccgcg ttcacgtctc gaagctccat
     6181 acgacgaaac cacgctcctc ttttcgaggc aagctcactc ggatactaca gtagcccgtc
     6241 gtcaggggcg agcgcggttt cgcccgcaga ggagtggggt aaaggcccat gcatgagaat
     6301 tgttgtggtg ggggcgacgg gcaacctggg gacgagcgtg ctgcgctccc tcgcggacga
     6361 gcaagaagtt gaatcggtgc tcgggctggc gcgaaggacg ccctcgctcg ccatgccgaa
     6421 ggtcgaatgg gcttctgttg acgtggtgaa cgatgatctc accccgcact tcagaggcgc
     6481 cgacgccgtt gtgctcctcg cctggatcat ccagccctct cgcgacctca ataaacagtg
     6541 gatggtgaac gtcgagggga gcacgcgggc cgcaagggcc gtacaggagg ctggagtacc
     6601 ctcgctcctc tacgcctcgt ccgtcggcgc gtactcgccc ggtccgaagg ataggcgcgt
     6661 ggacgagggc tggccgaccg gcggcgtccc gacctcgtac tacggacgcc acaaggccga
     6721 ggtcgagcgg aggctggacc gcttcgagcg ggaggcacct ggagttcggg tagtcagaat
     6781 gcgtccgggt ctcgtcttca agaaggaggc cgcgcagggc ataaggcgac tctttgccgg
     6841 gccattcttc cccggcttgc tcgccaaccc ggccctgatc aacatcgtcc ctgagataga
     6901 ggacctgcgc tcacaagttg tacattccta cgacgtcgga gaagcatttc ggctcgccat
     6961 cgtgaacgag gacgcgcgcg gcgctttcaa cctggcggcc gagccggtcc tggactcgcg
     7021 ggagatcgcg aggatactcg acgcccgtac cgttcccgtc cccgaacggc tcgcccgcgc
     7081 cggtgcgggg ctctcgtgga ggctcaggat gcagcccgtg ccgcctggat ggctcgacct
     7141 ctcgcggcag gttccgatca tggacacgac gcgggcgcgg accgagcttg gctggacgcc
     7201 gcgttactcc gctgactttg cgcttctgga tcttctggag ggcctgtgga ccggcgccgg
     7261 cctcgacacg ccgccgctcg cgcggaggac gagcgggcct ctgcgaattc gcgagatact
     7321 caccggagta ggagggaggg agccgtgaag gcagtagtct ggcacggcaa ggaagacgtc
     7381 agggtggaca acgtccctga cccgataatc caggagtcca ccgatgccat agtcaggatc
     7441 acctcgaccg ctatctgcgg ctcggatctg catctttacg caaagctctc gccgctcatg
     7501 cgcgaggggg acatcatcgg gcacgagccg atgggcatag tcgaggaggt tggctccgac
     7561 gtggggcaca tcgcgcccgg cgaccgcgtc gtgatcccct tcaacatctc ctgcggccac
     7621 tgttatttct gcgaccagca cctctactcc caatgcgaga aaacacgtga tacgggcaag
     7681 ctcgcccagg cggtcaacct cctcggacgc ggcaggggcg cgagcctcct cgggtacacg
     7741 cacgtctacg gcgccgtccc cggcggacag gccgagtacc ttcgcgtccc gcaggcccac
     7801 ttcggtccca tcaaagtacc ccaaggaccg ccggacgagc gctttctcta cctctccgac
     7861 gtactgccga cctcctggca ggccgtcgtc tacgctgacg tgccagaagg cggcactcta
     7921 ggtatctggg gcctcggtcc tatcgggcag atgtgcgcca ggatggcgct ccaccagggg
     7981 gccggccgcg tcatcggggt ggacaacgtg gccgagcgcc tctcgctcgc ctccaggtgg
     8041 ggcgtccaga ccgtggactt ctcggccgtg gacaacgtga gggatgtgat cctggacctg
     8101 acggagggac ggggtctcga ctcagtcata gacgccgtcg ggatggaggc cagcgggtcg
     8161 gtcgttgggt ccattctcca ggctaccaag ctccagctcg acagggcgac agcactcagg
     8221 gagtgcatgt ccgccatcag gcgcggtggt acgctctcga tctccggcgt gtacgccggt
     8281 ccgatccagg ctttccccct cggcgacctg ttcgacatgc aggtacaact ccgtatggga
     8341 caggccaacg taaagcgctg ggttgacgac ctgatgccgc tcctcggaga cgatgacctt
     8401 ctcgggaccg aggacctcaa gacccacgag ctaccgctcg acgacgcgcc gctaggctac
     8461 gagatgttcc agaaaaagcg ggacggtgcc atcaagtgcg tcctgaagcc ataggttgtt
     8521 gcactcacga cgcgccgacg gtgtaggcga ccgtggctgc cggaaagaaa agcggcaccg
     8581 aggagcgtgg gacgaagcgt agcgtctacg cggcgttcac ggcgaacctc ttgatcgccg
     8641 tctccaagct catcgcgggc cttatctcgg gtagcgcggc gctgctggcc gagagcgtcc
     8701 actcggtggc tgacaccata aaccaggtct tcctgctcgt gagcctgcgc tcggcggacg
     8761 aggagcccga cgacgagcat cccttcggcc acggaaagga ccgcttcttc tggtcgctgc
     8821 tcgtggcggt ggggctcttc gtcgccggct cggtgttctc tatctacgag ggcgtctcca
     8881 agatcctgca cggctcaggc tcgcacgacg cgttctttat cgggtacgtg gttcttgcag
     8941 cggcgttcgt cttcgagagc ggcgcgctac tcgtaagcgc gcgcgagttc aggcggaccg
     9001 cgcacgaggc caacgtctct tttcgagaac acttcaggac cacgcgcaat acaacaatga
     9061 aggtgccgct ctacgaggac gtggccgcga taatgggcat cgcggttgcc gcaacagggc
     9121 tctttctgac ccagatcacg ggcaaccacg tctacgacgg gctggcctct atcggcatcg
     9181 gggtaatcct cgtcttcgtc gcctgggagc tgggaaccga ctcgcggagg ctcctgatcg
     9241 gggaggcaat gccgccagag acgcgccaga agatggagga gatcgtgctc tccttccccg
     9301 aggtcgttga ggtactgcgc ctgctaacga tgcacctcgg gccgaacagc gcgctcctga
     9361 cggccgagat aaacgtcgtg gacggcctga ccacggacga gatcgaagac ctcctcgaat
     9421 gcatcaccca gaaggtgcgc tccgatgttc ccgaagttac ccagactttc atagagctcc
     9481 accccgaagg aagcgtggga agttactgat cctggtgtaa tctttcggcg gccaggacga
     9541 tcccgtcgtt atccgcatag acgtagtggc ccggcgtcca cgtaacgccg ccgaactcaa
     9601 cgggcacgtc cctctctccc tcgccttcct tcttgctcct caatgggtgg ctcgccagcg
     9661 caagtatgcc aacctctatg tcggcaagtt cccgcgaatc ccggacgcag ccgtttatga
     9721 cgatcccggc cagacctcgc gatgcggcga tcgaggccag cttgtctccg agtagggcac
     9781 aacgcctgga accgccgccg tcaacgacga ggacgcttcc cggcgcgaga tcgtccaacg
     9841 cctccctgac cagcacgttg tcctcgtaca cttttgccgt cgagatccaa ccgtcgaaac
     9901 catcgaccga accgtaggag gtgaacatgg gctcgcagac ctcgacgctc tctgtgaaac
     9961 ggtcgcagat gtccgccgtc ttgataggca tattcttcgt actcaacgcg ccgactcaac
    10021 cacagcccgc cagaactcgt cccgctcacc aggcacgaag gggagataga gcgcgacgcg
    10081 gtctatgagg ccctcgtagc gctccttgag cgcgcctccg atctcatcgg gcgcggcctc
    10141 gacggcgaac gccgctagca tctcgtcggt gacgagatcg ggcatctcgc gccacttctt
    10201 ctcgcgcgcc atcgtgccga gcctctcgcc gacctcttcc cagccgtggg cttcgaggac
    10261 ggtacggtag gtcggggtcg aagcgtagaa agagatctgg gcccgcaccg actcccgctg
    10321 ctctgcggct tcctcttcgt tctgcgcggc gatgacgaaa gcgctcgttg caagcacgac
    10381 ctcctcaggg tcccgaccag cttctcctgc accctctgat atggctggtt tgacggtacg
    10441 tcgcacgtac tcgggggagt ggaaagggtg gacgtggaag ccgtcgcagg cctcccctgc
    10501 gagcctggcg agtcgggtgt tgactccagc tatgtagacc gggatctccg ggtgctctat
    10561 gggaccaggg ttgaagaacg ggctcatcaa ggtgtgcctg tagaactcgc catcgaactg
    10621 aagggggcct tctgtccgga acgactccca gatcgcgcgc agcgccagga tgtactcgcg
    10681 cagacgcgcc gccggcctgt cccacggcat cgagaagcgg cgggttatgt gggccttgac
    10741 ctgcgtgccg aggccgagca cgaaacgtcc gtcggagagg tcctggaggt cccacgccgt
    10801 ctgggccgtc tccatcggag accgcgagaa ggcgatggcg actgaggtgc ccagctctat
    10861 ctcgtgcgtc gcgttcgcgg cgatagccag gggcaggaaa gcgtcgtgtt tggtctcgct
    10921 ggtccacagg ccggcgaagc caagggcttc ggctgcccgg gccgtccgat ctgtcgcttt
    10981 gagtttgccc tcggtcccga ggccggcgtc aagcttcatg gtgtcgccta cggtatgagc
    11041 agcagtttgc ccgtagtctt gcggccttcg aggtcgcggt gggcctgctc cgcgtccgcc
    11101 agggagtagg tgctgccgat tcggacgctg aggttgtttt gcccgatcca ggaaaacagg
    11161 ctctctgccc tccacaggag ctcctcgcga tttgtggtgt actgggcgag ggagggccgg
    11221 gtcaggaaga ggccgccttt gtggttcagg acctgggggt ccaacggggg cacggggccg
    11281 ctggatgccc cgaagagcac catgtatccg cgccggcgca gcacgtcgag gctgccgtcg
    11341 aaggtgtcct taccaacgga gtcgtagacg acgtggacgc cctcgccgcc cgtgatgcgg
    11401 ttcgtctcct ctacgaagtc ctgctcggtg tagaggatga cctcgtcggc ccccgcctcc
    11461 ctggcgagtt gcgccttctc ctcggtgcct gcggtcccga tgacagtggc accgcgcaac
    11521 ttcgcgagct ggacgagcag gagcccgacg ccgccggcag cggcgtgcac gagcgccgtc
    11581 tggccctcct gcagcgggaa ggtgctgtgc gtcaggtagt gggcggtcat gccctggagc
    11641 atgaccgccg cggccacccg cgcctcgacc agcgtgacgt taaaaggcac caggttctgg
    11701 gccggggcga ccacgtactc cgcgtaggag ccgggcgtgc tcgcgaaggc tacgtagtca
    11761 cccggcgcaa tgtcctcgac gccttcaccc accgcttcca cctcgccggc gccttcgagc
    11821 ccgagcgtgc gaggcagatc gccggggtag aggcccttgc gctggtagac gtctatatag
    11881 ttcacgcctg aggcggcgag cttcacgcgc gcctgaccgg gaccgggctc ggagacctcg
    11941 acatcctcgt agcgcaagac ctcaggcccg ccgaactcgt gtatacgtac agccttcata
    12001 agtcactcct ccccacgatg aactggactt tattgtaccg gcaatctcag gagcgccgcc
    12061 ggacaagcac gcagtacgaa tgctagacgg ggagtagatc tgcggcgccg gtccacctga
    12121 agactacggg tgatcgggtg tgcccggatc tagtcctttc cgtgcttgtg tccttttccc
    12181 tcgtccagcc ccctggccgg cgccgccagg ttcggccctc gcaacgaggg tcccgctttc
    12241 accttgacct ttggaccttt gaccttggga gccttctcct cagcagcctg attctcggct
    12301 ggctggcgtt cctcctcctg ggccggttgc gccgatgcgg aagaagcggc ggtggcggag
    12361 gccccggcgc ttgactgggc cgacgcggag gcgctcgccg acgagcttgc ggacgaggac
    12421 ggctgcggtc ccgagctgat ggtcaggttt acttcggtgc ctggctcgac cggggtgcct
    12481 gcttctctgc cggccgagat gactctgccc tcggcgaccg tgtcgctcgg gaacgagcct
    12541 acctgaccga cttcgagccc ggcgtcggtg agcctctgct ccgcctcgct cctggtgagc
    12601 ccggccacgt ctggcacctt caccttctcc gggccggaag gctgggccgg agaaggttgc
    12661 ttgcctgcct ggaagacgtc gaaccagccg ttcatggcgt cagccccgag gacggcctgg
    12721 aggccgccga gcacgcccag ggtaaacagg accgcgacca gcgcgagaag cagccagggc
    12781 aagccgcgcc ggcgccgcat cggagcagta cgggcgcgtg ctgtcggcat cttgctcgca
    12841 aagtattccg gcgccaccgc gttcggccgc gtgcgggctc cctgcatctg gtcggcgccc
    12901 accggtggga gcccgcgctg caccctccac aggtcgtcgg cgagctcgtc ggcgctcgcg
    12961 tagcgatcgt ccgggtcttt cgccatgagc ttcgtaacca gcgcgttcat gccctcggtt
    13021 atccggggat tcaattctct cggaggccgc agcggctcgt tcacgtgctt catggagacg
    13081 gcgatggggt tttcagcggt gtaaggcagc tcgcctgtga gcgtctcgta gaggactacc
    13141 ccaagcgaat acaggtcgct cttgggtgtg gccggcttgc cgagtgcctg ctcgggagac
    13201 atgtagcctg ccgtgcccat cacggacccg gtctgggata tcgttgccga ggaggccgcc
    13261 ctggcgatgc cgaagtcagc caccttcgcc tcgcccctgg cggtgaggag aacgttctgg
    13321 ggcttaatgt cgcggtggac catgcccctg tcgtgggcgg ccccgagcgc ctccgctacc
    13381 tgagccccga tcgcggccgc gtccgccgtt tcgagcggac cctcctcgga gatcctctcc
    13441 ttcagcgtgc cgccgggcac gtactccatc gctatgtatg aggcctcttc ttccgtctcg
    13501 cccctgtcgt agacctggac gatgttcgga tgcgataggc tcgcggcgct cagggcctcg
    13561 cgtttgaagc gctccgcgaa ctcgttatcg ccggcgtact gcctcctgag caccttcagc
    13621 gcgacgtcgc gcccgagcac cctgtcgcgg gccaggtaca cctcacccat gcctccgctg
    13681 cccagtggtc gaacgaggtc gtaacggttg tctacgacag tttttcctac gtctcgcatc
    13741 ctgcccttgt gtctagcctc aagtctgcta ccgcgacatt ctacacgtcc ggtaccggcc
    13801 ttacgaagaa cgagggttag acggcgtccc cgcagggtac ggttacaatc ctagagcgaa
    13861 cgacggatca ggaggacgac gtgggacgaa gaggcagggg cgactccgaa agaccgggca
    13921 ggcaagaggg gggacagggg cgccagcgtg accgctccga aggcgctgcc gaccgccacg
    13981 cagccaggga cggcggccgg cccgaggagc gtgaggatgc cgggtacaag gacagggtcc
    14041 acgagcgcct cgagcgaatg gacgcgcgtc tcgagaggct cgaacagcgc ctggagaagc
    14101 tcgatggcgg gggtgcctga ggaggcgtcc atcgtgtgga agatctcagg ccggggaggg
    14161 taggatcttg gcgacgagca gggtagaaga gaacgcgctg cgtcttcttg gcgctttcta
    14221 cgacctgtcg ggagagatga tcaccgagcc cgtgacgatc ggtgggcctg agatgccagg
    14281 acagggcgcg gctccaaggg ccgggctcga ccccgagtcc acagagtgcg acgtcgcggc
    14341 caggtacctg gtgaaccagg gatatatcca atctaccggt gccgcttcgg actatatgat
    14401 caccgttgcc ggtttcgaca gggtgagaga gatgcgcggc atcggcgatt cggcgggaga
    14461 gaggagcggt atgagcgaca agacccagaa gcggcttctg acgctgctgt ccatagggat
    14521 ctccgtgggc ctgagccagc cgctgaccag gttcatcggg gaggagatcc ccgaacgcag
    14581 gggcattaga gacgacgtga cggaggctgt cctgaagggg ctcgtcaggg cggtcgcgct
    14641 cgtggcggcc tcgatcatcg ttcggcaact ggcggcgagc cggaggtagg gacaagacga
    14701 tctctattcc tcgtcggtcc tcaatgagga gataaacgat ggatctggct tcgggggccc
    14761 agatcctgtc ttccgtcgcc gtattgctcc tggccgtatt cgctttcgtt caggtgattg
    14821 agcagcgcgc ggcccgcata gcccaggaca gcccgcagat tctcgtcgac gtgaactaca
    14881 gccagcgcac aatgatcaac atagtcgtca ggaacatcgg gaggggggcg gcgaaagaca
    14941 tcaggttcga cttctcggcg cccgtcgaaa gctcaggcgg gcagaggatc tcggacctcg
    15001 cctatttcaa ggagggcata gctttcctgg cgccgaacac ggagatcgcg accgtctggg
    15061 actcgtacgg cgacgccgtc gaggtcttga agaagaaggg cctcaggaac ggcataacgg
    15121 ttactgtgaa gtacaaagac ctcaggggga aggcctacat tacgcggtgg acgatcaacc
    15181 cgctgatcct ggagggtagc ggttacgccg attacaggga gttcgaggac ctggtgaggg
    15241 cactggagaa gatctccagg gatatcgagg gcctgagaga ggcgacggtc cgtctcgaag
    15301 cgccgcggga ggagagcgat ttggacccac gttaggccgt cctctggcgc tcctctgata
    15361 tatttggctt gaggggcagg ttctcacgaa ggggagaggt atcatggcta cggtccgcga
    15421 ggcgaccttc gatctcttac gccgtcttgg gataacgacg gtcttcggca acccaggctc
    15481 gaccgaactg ccgttcctga aggacttccc cgacgacttc cgctacgtgc tcggtctgca
    15541 ggaggcgacg gtactcggga tggccgaagg attcgcccag ggcacgggac aggcggccct
    15601 ggtcaatctc catacaggac ccggcctcgg caacgcgatg ggggccatga tcacggcctg
    15661 gcacaacaag accccgctac tcgtcacagc aggacaacag gacaggcggc acagtgccct
    15721 tgagccgttg ctgaccggcg ggctcgtgga actggcgaag ccctacgtca aacgcagctt
    15781 cgagcccgtg cggtccgagg acgtgccgcg ggagatcctg cgcgcctacc acaccgcgat
    15841 gcagcagccc cggggtcccg tcttcgtctc gatcccgatg gacgactggg actccgacgc
    15901 cgaactgctg gaagaccacg acgtggtcca cagaatcgct ccagatcccg acgctatcga
    15961 acggttcgcg agtatcctcg aggaagcccg gagccccgcc gtcgtcacag gagcaggcgt
    16021 cgatcgggcc ggggccttct acgacgcggt cacgctcgcc gagaagctcg ccgcgccggt
    16081 atggcaggac cctatctccc cactcgccag cttcccccag gaccatcctc tgttccaggg
    16141 ccatcttcct ccggcccaga gacagctcgc cgaacaactc tcgggccacg acgtcgtcct
    16201 cgtcctcggc gcgcccgttt tcctctacta tccgtacgta cctggcccga ccgtcgcgga
    16261 cgggacgcgg gtgctccacg tctccgagga ccccgaggag gcggcccggg ccgcggtagg
    16321 gacgagcgtc gtcggggacg ttgggctggc gatcaagggt ctcgtcgagc tgttgcccga
    16381 tgcgtaccgc ccaccacctc agtccaagga gccacctccc gagcctgagg ccggaacgcc
    16441 gatgtctgtg gagtacgtga tgcacaccat agcgggtgta ctccccgaag aggcggtcgt
    16501 cttcgacgag acggcctcga gcaaggcgaa gctgcacatg tacatacgca tcaaccggcc
    16561 cggcggctac cacacctcgg cgacgggcgg gctcggcttc tgcatgccag gctccgtcgg
    16621 gttcaagctc gccgtgccag agaggcccgt tgtatgcgcc gtcggtgacg ggtcgagcat
    16681 gtactcgatc caggccctct ggagcgcggc ccgctacggg gcagccgtcg ccttcatcgt
    16741 cgtgaacaac aggggctact ccatcctcaa gggcttccgt gacgccatcg gcgcagggga
    16801 cagggtgccg gggctcgacg tggaggggat agacttcgtc ggcgtcgcaa agggtttcgg
    16861 ggtcgaaggc gagctggtgg aggaggcagg ggatctcctc cccgccgtca agcgggcatt
    16921 gaccgcaggt agaccgtacc ttctcgaggt cgttgtcgac cccgaagtcc cgagtctcct
    16981 gagctagagg ggatcccgaa tggcagacgt cttcgtcgtc ggttcgatca accaggactt
    17041 cgtcatgtac gtggatcgcc gtcccgagcc gggcgagacc gtcacggacg cccgtctcgc
    17101 gacgcacaac ggcggtaagg gggccaacca ggccgcggct gcggccctcc tcggcgcgag
    17161 cgtagtcttc ctaggacgcg ttggcgacga cgggttcggc gagccgctgg ttgaggcgct
    17221 cgccgacaag ggtgtcgatg cgagcctgat cgaagttgcc cccgagtcct ctacgggcac
    17281 cgccttcata accgtcacgc cggatgggga gaacgcgatc acggtcgccc caggcgcgaa
    17341 ccgccgcctt accgtggaga acgtggatgc tgcctccggt tccatcggag aggcacaggt
    17401 gctcgtcgcc cagatggagg tcccgccgga ggtcgtcctg cgggccgtcg aggtcgctgc
    17461 ccggagcggg acccgcgcgc tggtcaacct ggcgcctcct ttcgaggttc cgcgggagct
    17521 gctggacaaa ctcgacccca tcgtggtcaa cgagcatgag gccgccttcc tgctcggcga
    17581 gagggtagaa ggagtggatg gggctctctc cgcagctcca gagctcctgg ctctggggcc
    17641 gaagtcggct gtgatcacag tcggagaagc cggcgccgtg ttcgcggaag gtaagtacac
    17701 aaaacacctt ccagccccga aggtcgacgt cgttgacacc acgggcgctg gagacgcctt
    17761 cgtcggcgcc cttgccgcgc aactcgccag aggctcctcg ctcgaagacg ccgtagccta
    17821 cgccgtgcgc gccggagcgg cggctgtgac gaaggaaggc gcccagggag cgctcccgac
    17881 gccagagacc gtggaatccc tgtagtacgc gcctgtattg aacggtgtgt tcggcactac
    17941 gtccggctgg tagctcctgc gaagaagacc gcgccgtgcg tctgaaccgg gtcgggctcg
    18001 aagccggcgt ggtcgaggag gctccgtagc tcgtcggggt cgaagaactt gaggccaccc
    18061 gtggagagga agcgctggag gcggcgccca cgcggcgtgc cggcgcgaag gatgcccatc
    18121 atagcgaggc gtcctcctgg ttcgaggacg cggtgggtct cgcgcaggac cctggcaggg
    18181 tcgccgaact cattgagaga gccgccgcag acggcgccag cgaaacaatc gtcgatgaaa
    18241 gggaggttca cggcgctcgc cctgacgaag gaaggtttca cgcctgtcgc gcgggagcgg
    18301 cgggcggcct ccctcagcat ggatggggag atgtctatcc cgacagcggc gccgtcgtcg
    18361 agatcccgtg caaggctgcg ggtgtagagg ccggaggagc agccgaggtc gaggtagcgg
    18421 gcaccgcgaa ctgggcgtac caggtctgtg atgatctcca gctcccgctc ggttgggaaa
    18481 gattcgcccg tgagcaggct gagggagtgt ttgcgccaga gcggttcgta gcctcgtcct
    18541 gcgccgggga tgaagttggt gaggttggcg acgttggccg cgccggcgcg ggttttcagg
    18601 aggtccaggt agccgttctt tacttcatag gtgtcgctgg ttacggggga gcgggctttg
    18661 ccggccatga ccatcccgtc ttcctctata cctccggagt agatgagtcc cccgccgtct
    18721 gaaagacgta cgatcgagag caggtccagc cccatcaatt agcggcgtct tctgtcgcgc
    18781 aggcggttca ggcgggcgtc gaaacccctg acggagtcga tgaaggcacc cgactcctcg
    18841 ggacggggta cggcgtttag cgtggggtag ttgccctcgt ggaagacgag ggggcctttg
    18901 cgtgtatcgc ccatacggac ttcgactacc ctgccgagga acaggtcgtg gtctcctccg
    18961 gcgtagatct cggccagctc gcactctatg accccgaggg cgccggctgc cagcggtgcc
    19021 cccgtcgagc cgtagccgcc gcctagagag tggagcgcag gcagaccgct tgagcgctcg
    19081 ggcgaggcga agagcctgct gagtccttcc tggtcgtccg cgaggaggtt caccgcgaag
    19141 tagccttcct ccctgatgcg ggggttcagg cgagcgtcct tgtggacgct gacgaggaac
    19201 agcagcgggt cgagggagac ggagatcacc gcgttcgcgg tcatgccctc gaccttctcc
    19261 tcccgcccgc tcgtcacgac cgtcacgccg gtaggatagt ggctcataat gctccgcaaa
    19321 gcgtcggcgt cggcgtccct gacctcccag ggatactctc gctcgttcac accactcggg
    19381 ccgtcaggcg gcatctttgc cctttcctgt acgccggatc ctcaggaggc tactacgcac
    19441 gagtgtctcc cacaagagaa cctccgacgc gcaactcttc gggccaccac cgctcgaccg
    19501 tctctctatc tgatccgtac cagaggccga gacgcctgtt gtggtggacg ccgtccacga
    19561 ccaggctctc cgagccaggg agcaccgctg catcgacggg cacgaccccg tcgccggcca
    19621 tgcagccgtc ttcgacgaag cgctcgtagc gcttgcgggc cttcggagag gaggcgccgt
    19681 cgacggcgct tcccgcgacg gagaggtagc gcaggccatc gtgcagcgct ccggggaaga
    19741 gttcgttcac gcgggtgatg ggggagaggc gttccctatc cgccacgttg tgcggggtcc
    19801 ccagtgtgat caggcgctcc acccgcctgt gacctgagta gcgccgcccc ccgtaaggag
    19861 gaactccgcc gatatacacc cggcacgcga gtccgccagc cgagtgtccc acgagcaccg
    19921 ctcttttcgc ttcgctctcg agcagcgccc tatccaccgt gctcgccacc tcgaagacaa
    19981 gttgcccgaa gccccggaag tatccgagca cccagtccag gggcgtaacc ggagccacgt
    20041 gcacctcgga gccggagatc tcgcgcagca tattggcgaa gtccctgtaa ctgcccggcc
    20101 accatgtagc cccaccgacg atcactattg gcgcgctcga catgccgaga gtatatccgc
    20161 ccgtcaggcc aggctcttat ggagaccgtc caggacgaac tggacggcga gcgctgcgag
    20221 caagacgccg aggacgcgcg aaagtacgtt cgccccggtc tccccgaaga gctgcataac
    20281 gcggggggcg aggagcaacg agatgagggt cagaaccagg atcacgaaga gtacgcccat
    20341 gaataacaac aattggcccg tgctgcttcc accggtgtac aggaggaccg tggtgatcgc
    20401 accaggtccc gccagcaacg ggatggcgag agggaacacc gagatgtcct gctggtaaga
    20461 ctcctcctcc tgctcgcgca ccgacgtggc gcgcatccca gaaggtctcg cgaagaccat
    20521 gtcgagcgaa aggaggaaga gcagcagtcc accggcgatc cggaacgcag ggagcccgat
    20581 gccgagagcc tccagcagca cgtcaccagc aagggcgaac acgtagagga tgagggcgcc
    20641 gagcagagtg cccctgatgg ccgactcgcg cttctgcttc ttggagaaac cgcgcgtgag
    20701 cgcggcgaag atgggggcga gacccagtgg gtcaacgacc acaagaagcg tcacgaaagc
    20761 gttgaatacg aagtccataa tcgtaagact ttacctcaca atcacgtccc tgtttcgggt
    20821 gcaacgcgcc actcacccct caacgaccgc gcccgctcca gggtcttcgc cccgatccgg
    20881 ccttccgctg ccacgtaacg attgcgcacc tcttccgaga gcgcctgtat tactcgtgca
    20941 acgtctaccg gcagatcccc gacgctcccg ggccggaacg gtagacccaa agcttcgtag
    21001 aagcgcccga ggacggcccg tatctccgcc gtctccgaga ccaggatgag ggcttcgagc
    21061 cgcgcggccc tgcgtgtgac ccgctgggcc aggcccgcgt gcttgacgcc ccacggaccg
    21121 ccggagcgca cggagtacgc cccaggacag aactccccgt cgacctcgcc gcgctccgcg
    21181 tcgactccga cttgttgtaa tgccgacgcg atgagatcca agccctctga gtagcgctcg
    21241 aagaggccgt gacgcaagtc ctcaaccgga aacgtcagcg agaacgacag agagccccga
    21301 ttcgccgcga ccgctccacc tcccgagtta cgaaccagca cggggaagcc gaggtccgag
    21361 gccgccctcg ccgccgcttc gaagcccgcc tgacgcgtct cctgccgcgt cgccccgacg
    21421 tagcgcgagg aggtccacat cagggctaca ggaccacgtt cacccgccgc gacctcctca
    21481 agcacggcct gttgcagccc gaagccgtct tcggggtcgt cgtatggctc tgcggctacc
    21541 agaatctcca tttgcctact tgcgggcaac gaacgtgcct acgtcttttg tgatgtggat
    21601 agcgcctcga gaagccagct cgaattggtc gaagatccag cgtgtcaggc catagaagct
    21661 ggtgctgaac cgttcgtgca gtgcgatgcg tgcgttcaga ttcgaagggt cgcggtattg
    21721 ccgccttacg tattccgggt ccgagtacgt cacgctttgc ctcctggctt cgaccggctg
    21781 gtgcacgacg tcggctggtt tgatcttcca aaatgctacg atagcaagcg tggtagacgc
    21841 cggctagata cttgcggccg ggaaagggtc agatgcctcc cgacgaaata cccgtggtcg
    21901 atacagaagc ggtgctgcag acgcctcgat tagtgatcga acccctcctc catgctcacg
    21961 ccgtcaggct ttacgagtgt ttgcaggaca agcaattgta cgaatatatc ccccaggacc
    22021 ctcctgcttc gccacaggca ctggagggcc gctatctgag cctgtcctcg cgacgctctc
    22081 cggacaaccg cgaggcatgg ttgaactggg cgatgcgcac gcgtcgctcc ggagagtatg
    22141 tcggtgttct ggaggccacg gtacacgaag acctgactgc tgcggtcgcc tatatggtct
    22201 tcgtgccttt cagcggcggg gcctcgccgc agaagcgtgt atgcgcctgc tcgcgtatct
    22261 tttcgaggac tatcaggtga acgtggccgc ggcggagata gacacgcgca acgtcgcctc
    22321 catcgcgctc gtcgagtctc tgggtttcga gcgcgtcgct tttcacaagg ac
//
LOCUS       DATAIR010000573         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_63544, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000573 DATAIR010000000
VERSION     DATAIR010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63544"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHM69_13710"
     CDS             complement(<1..240)
                     /locus_tag="VHM69_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2741496.1"
                     /translation="MKEAGNTPVDASATEHGYIKEGDKEKIQNRLRRIEGQVRGIQRM
                     VDEEAYCVDILTQISSVVSASEKVATILLKDHVEHC"
     gene            complement(250..2676)
                     /locus_tag="VHM69_13715"
     CDS             complement(250..2676)
                     /locus_tag="VHM69_13715"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2741497.1"
                     /translation="MDKSLRKVAFPVTGMSCAACARRVEKALVRTEGVSAANVNLAVE
                     KATVEYDPASAEPDALIRAVEDAGYGIEKHKTGFGVTGMSCASCVGRVENALGRLPGV
                     LGVNVNLAAERVTVEYLAGAAEMRDFERAIESAGYGVVREEERTAEDERGREYRRLRG
                     RFLLAAVLTALVLIGSLPMMLGLMIPVPVRWLNFALLALATPVQFWSGWRFYEGAWGA
                     LKHGQADMNTLVAVGTSAAYLYSAVATLAPGLFQGRADVYFDTSSLIVTLILLGRLLE
                     ARALGRTGEAIKKLAGLQAKTARVVRDGREINIPVEEVEVGDVVVVRPGEKISVDGVV
                     VGGESAVDEAMISGESMPVTKREGDEVIGATINKTGTFRFRATKVGRDTALARIMRMV
                     EEAQGSKAPIQRLADRISGVFVPVVMALATLTFFVWLVFGPEPAFTLALLNFVAVLII
                     ACPCAMGLATPTSIMVGTGRGAERGILVKGGEALEVAHRLTTVVLDKTGTLTKGEPHL
                     TDVVVIDGFSEDGLLRMAASVERGSEHPLGEAIVAGAWDLDLAEPEDFDAPTGRGVRA
                     TVEGRSVLVGSRKLMDEYGIPEDGLSPELERLSGEGKTPVLVAVDGEPAGIVAVADTV
                     REESREAVDALKKMGLDVAMITGDNERTALAVARELGIEHVLAEVLPDQKSGQIKKLQ
                     AEGAVVAMVGDGINDAPALAQADVGIAIGTGTDVAMEAADLTLISGDVRGVVRAIRLS
                     RATIRNIRQNLFWAFAYNVVLIPVAAGALYPFFGEAGFLNPVFAAAAMALSSVTVVSN
                     ALRLRRARID"
     gene            2845..3570
                     /locus_tag="VHM69_13720"
     CDS             2845..3570
                     /locus_tag="VHM69_13720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2741498.1"
                     /translation="MRYGFVITSGDPRVVAELAGEVEEAGWDGAFYWDGICVGDMDTY
                     DPWVVMAAMAMRTERVRIGAILTPPARRRPWKLARETVALDHLSGGRLVLPVGLGALD
                     DGGFAKVGEPTDRRVRARLLDESLEVLTGLWSGEPFSYQGEHYRLEEMTFRPPPVQRP
                     RIPIWVVGAWPSQKSMGRALRYDGVLVATVGGSLESPGVTPGVIRQIKEYVEERKEGP
                     FDIVCEGQTPGEDPGRAASIVRP"
BASE COUNT          632 a         1262 c         1198 g          724 t
ORIGIN      
        1 gcagtgctcg acgtggtcct tgagcaggat ggtcgccacc ttctccgagg ctgagacgac
       61 gctgctgatc tgggtgagga tgtccacgca gtacgcttct tcgtccacca tgcgctgtat
      121 cccgcgcacc tgcccctcta tccgcctgag acggttctgg atcttctcct tgtctccttc
      181 tttgatgtac ccgtgttccg tcgcggacgc gtccacgggc gtgttccctg cttctttcat
      241 gtcgtcctcc taatctattc tcgcccgccg caaccggagg gcgttggata ccaccgtcac
      301 cgacgagagt gccatcgccg cagcagcgaa gaccgggttg aggaaaccgg cctcgccgaa
      361 gaacgggtag agggctccgg ccgcgacggg gatgagcacc acgttgtagg cgaaagccca
      421 gaaaaggttc tgcctgatgt tcctaatggt cgccctggag agtctgatgg ccctcacgac
      481 cccgcgcacg tcccccgaga taagcgtgag gtccgccgcc tccatcgcca cgtcggtacc
      541 cgtcccgatg gcgatgccga catcggcctg cgccagagcc ggcgcgtcgt tgatgccgtc
      601 cccgaccatc gcgacgaccg cgccttcggc ctggagtttc ttgatctggc cggatttctg
      661 atcggggagc acttcggcca gtacgtgttc gatgccgagc tcgcgcgcga cggccagggc
      721 ggtccgctcg ttgtcgcccg tgatcatggc tacgtccaac cccattttct tcagcgcatc
      781 cacggcctcc cttgactctt ctctcaccgt gtcggcgacc gccacgatgc ccgccggttc
      841 cccatctaca gcgacaagga ccggcgtctt gccttctcca gatagccgtt cgagctccgg
      901 cgagagcccg tcctccggga tgccgtactc atccatcagc ttgcggctgc cgacgagaac
      961 actcctgcct tcgacggtcg cccgcacgcc gcgccctgtg ggtgcgtcga agtcctcggg
     1021 ctccgcgagg tcgagatccc acgcccctgc gacgatggcc tctccgagtg ggtgctcgct
     1081 gccacgttcg acggaggcgg ccatgcgcag caacccgtcc tcggagaaac cgtcaatgac
     1141 caccacatcg gtcagatgcg gctctccctt cgtgagggtg ccggtcttgt ccaggacgac
     1201 ggtggtgagc ctgtgcgcca cttcgagcgc ctcgccgccc tttaccagga tgccgcgctc
     1261 cgcgccgcgc ccggtcccga ccattatgga ggtcggcgtc gcgaggccca tggcgcacgg
     1321 gcaggcgatg atcaggacgg ccacgaagtt cagaagggct agcgtgaacg cgggctccgg
     1381 cccgaagacg agccagacga agaaggtgag cgtcgcgagc gccataacga cggggacgaa
     1441 gacgccgctg atcctatccg cgaggcgttg tatcggggcc ttcgagcctt gcgcctcctc
     1501 gaccatgcgc atgatcctgg cgagcgccgt gtccctgccg accttggtcg ccctgaaacg
     1561 gaacgtcccc gtcttgttga tggtcgcccc gatgacctcg tcgccctcgc gctttgtgac
     1621 gggcatggac tccccggaga tcatggcctc atcgaccgcc gactcgccac cgacgaccac
     1681 accgtccact gagatcttct ccccaggccg cacgacgacc acgtccccta cctcgacctc
     1741 ctcgacaggg atatttatct cccggccatc ccgtacgacg cgcgcggtct tcgcctgcaa
     1801 cccggcgagc ttctttattg cctcgcctgt gcgcccgagg gcgcgcgcct cgagcagtct
     1861 tccgaggagg atgagggtca cgatgagcga cgaggtgtcg aagtacacgt cggcccgccc
     1921 ttggaagagc cccggcgcga gcgtcgcaac ggcgctgtac aggtatgccg cgctcgtccc
     1981 gacggcgacg agcgtgttca tgtcggcctg gccgtgcttg agagcgcccc aagcaccttc
     2041 gtagaatcgc cagccggacc agaactgaac gggcgtcgca agcgcgagca acgcgaagtt
     2101 cagccaccgc acggggaccg ggatcattaa tcccagcatc ataggcaggc tcccgatcag
     2161 gacgagcgcg gtaagtaccg ccgccaggag aaaccgcccc cgcagccgcc tgtactcgcg
     2221 cccgcgctcg tcttcagcgg tcctttcttc ctccctgacc acgccgtacc cggcactctc
     2281 gatagcccgc tcgaagtctc gcatctccgc cgcaccggcc agatattcga cggtgaccct
     2341 ctcggcggcg aggttgacgt tcacgcccag tacgccaggc agcctcccca acgcattctc
     2401 gacgcgcccg acgcaactcg cgcaactcat ccccgtcacg ccgaagccgg tcttgtgttt
     2461 ctcgatgcca tacccagcgt cttccacggc ccggatcaaa gcgtccggct ccgcggaggc
     2521 tggatcgtac tcgaccgtcg ccttctccac agccaggttc acattcgccg cggagactcc
     2581 ctcagtcctc acgagcgcct tctcgacccg ccgcgcacac gcggcgcaac tcatccccgt
     2641 gacaggaaat gcgacttttc gtaatgattt atccacttag cgaacctctg cctcagagcc
     2701 tgcgaaaggc agtgtaccat acccccctac cctatataaa gatcatgatg tgctccgaac
     2761 ttcggtgttt ttcgttcggc ggttggggcg tatacagagg ccccgtatac tcgtttatgg
     2821 gaaagtctat gagaggaggt gtacatgcgc tacgggttcg tgatcacttc gggggatcct
     2881 cgcgttgtcg cggagcttgc gggtgaggtg gaggaggctg gttgggacgg cgccttctac
     2941 tgggacggca tctgcgtcgg ggacatggat acgtatgatc cgtgggttgt tatggctgcg
     3001 atggcgatgc gcacggagag ggtgcgcatc ggggctattt tgacgccgcc ggcacggagg
     3061 aggccgtgga agctcgcccg agagaccgtc gccctcgacc atctatccgg tgggcgactc
     3121 gtgttgccgg tcgggctggg cgcgctcgac gatggcggct tcgccaaggt gggggagccg
     3181 accgacagga gggttcgggc gcgactattg gatgagagtc tggaggtttt gacgggatta
     3241 tggagcgggg agccgttctc ctatcagggg gagcattaca ggctcgagga gatgactttc
     3301 aggccgcctc ccgtccagag gccgcgcatc cccatctggg ttgtcggggc gtggccgagc
     3361 cagaagtcca tgggacgggc gcttcgctac gacggggtac ttgttgccac cgttggcggg
     3421 agcctcgaat cgcctggtgt tacgccaggg gtcatacgcc agatcaagga gtacgttgag
     3481 gagaggaagg aggggccctt cgacatcgtg tgtgagggac agacgccggg tgaagatccc
     3541 gggcgtgcgg cttccatcgt gcgaccttga gcggaggcgg gggcgacgtg gtggatcgaa
     3601 tctccgtgga tgccgccgaa cgagcctgaa gacctccgcc gccgcataga acagggtcct
     3661 ccgctggctg gctaaagtcg ctagcgggtg cgggtgtcct tcaggatctc gaagacctct
     3721 tcgatcaggg cctcgcgctt gtccggaggg aggtcgcgag gatggatagt ctgtttatct
     3781 gactggaggt tgcgcagggc cgcctcctcg gccagg
//
LOCUS       DATAIR010000574         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_408251, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000574 DATAIR010000000
VERSION     DATAIR010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408251"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VHM69_13725"
     CDS             <1..958
                     /locus_tag="VHM69_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2741499.1"
                     /translation="VAGALKVPYAAIELKQDGGFRTAAAHGSPAGEPTVVPLVRQNQE
                     VGRLVLSPRTPYESFSPADSLLLQDLARQAGVAVHAVRLTVDLQRSREHLITTREEER
                     RRLRRDLHDGLGPTLSGLVLGLNVSQRMMGKNPEEARELLSRLEKQSEEAVTEIRRLV
                     YGLRPPALDDLGLAAAIRRQAEALDLTDGPEARQPGDVAPVFSVTTPEELPPLPAAVE
                     VAAYRIVQEALTNVAHHASARDCQVRLSVDEEAGVLDLVVEDNGVGLQVDRRAGVGMI
                     SMRERAEELGGSCEVEAVPSGGTRVLARIPIPAWENDDREAT"
     gene            955..1605
                     /locus_tag="VHM69_13730"
     CDS             955..1605
                     /locus_tag="VHM69_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741500.1"
                     /translation="MKHRVLVADDHAVFRHGLRALLDSEPDMELVGEAATGREVVESC
                     AKLAPDVVLMDLQMPGINGIEATRRILQANPRVGIVVLTMFEDDDSVFSAMRTGARGY
                     VLKGGKPSEILEVVRAVAKGEAHFGPEIARRLTGFFSAPKIDPSEAFPQLTTREREIL
                     DLIAQGHSNAKIATRLYLSPKTVRNYISRIFTKLQVADRSQAIVRARESGLGREGH"
BASE COUNT          345 a          607 c          592 g          296 t
ORIGIN      
        1 cgtggcgggg gccctcaaag taccttacgc ggcgatagag ctgaagcaag acggcgggtt
       61 cagaaccgcc gcggctcacg gctcgcccgc gggcgagcct accgtcgttc cgctcgtcag
      121 gcaaaaccag gaggtaggac gcctggttct ctccccacgt accccgtacg aatccttctc
      181 ccccgccgac agcctccttc tccaggacct cgcccgccag gccggggtgg cagtccacgc
      241 ggtgaggctg actgtggacc tccagcgctc acgagaacac ctgatcacga cgcgcgaaga
      301 ggaacgcagg cgcctccgtc gggacctgca tgatgggctt ggccccacac tctccggtct
      361 cgtcctaggg ctgaatgtct cgcaaaggat gatgggcaag aatccggagg aagcgaggga
      421 gttgctctcc aggctagaga agcaatcgga ggaagccgtg acggagatac ggcgcctggt
      481 ctacggtctc aggccccccg cgctcgacga cctcggcctc gcagccgcca tcagacggca
      541 ggccgaagcc ttggatctca ccgacggacc ggaagctagg cagcccggcg atgtcgcgcc
      601 ggtcttctcc gtgacgacgc cggaggaatt gccgcccctg cccgccgcgg tagaggtggc
      661 cgcctaccgc atcgtccagg aggcgctcac caacgtcgcc caccacgcga gcgctcgcga
      721 ctgccaggtg cgtctctctg tggacgagga ggcgggtgtc ctggaccttg tggtggagga
      781 taatggcgtg ggactgcagg tggaccgcag agccggagtg gggatgatct ccatgcgcga
      841 gcgcgccgag gagttgggcg gttcgtgcga ggtcgaagcg gttccttcgg gcggaacgcg
      901 ggttctggca cggataccga tacctgcttg ggagaacgac gatagggagg cgacgtgaag
      961 caccgggtac tcgtagctga cgatcacgcc gtctttcgcc acgggctcag ggcgctgttg
     1021 gactcggagc cggacatgga gctggtagga gaggccgcaa cagggcggga ggtcgtggag
     1081 agttgcgcga agctggcgcc cgacgtggtg ctgatggacc tgcagatgcc agggataaac
     1141 ggcatagagg cgaccagacg catcctgcag gccaacccgc gcgtcgggat cgtcgtcctc
     1201 accatgttcg aggacgacga ttcggtattc tcggccatgc gcacgggcgc ccgcggctac
     1261 gtgctcaagg gcggtaagcc ttccgagatc ctggaggtcg tccgcgccgt cgccaagggc
     1321 gaagcccatt tcgggcccga gatcgcgcgc cgcctcacgg gcttcttctc agcccccaag
     1381 atcgacccat cggaagcttt cccccagctc accacccgcg agcgcgagat cctcgacctg
     1441 atcgcccagg gccacagcaa cgccaagatc gccacgcgcc tctacctcag cccgaagacc
     1501 gtgcgcaact atatctcgcg catcttcacc aaactgcagg tcgccgaccg ctcccaggcc
     1561 atcgtccgtg cccgagaatc cggactgggc agggaagggc actaaccgac actcaacttc
     1621 attccttcgt cggtgccact cccagcggct gaataagggc ccttctgtag ggacaaacag
     1681 ggacctcagg cgggacaacc cgtgaacaat ctcccagcca ggaagggacc tgtctcccac
     1741 gaccgtggga ccatgcccag gctagcgtgc ttgtggtatg aaaaagttcc acaaacgcac
     1801 cctgaccctg gcgttgtctc tcttcttcgg ccttggcttc
//
LOCUS       DATAIR010000575         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_131594, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000575 DATAIR010000000
VERSION     DATAIR010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131594"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VHM69_13735"
     CDS             complement(<1..462)
                     /locus_tag="VHM69_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741501.1"
                     /translation="MSTDDRVAIVTGASRGIGRQVSLTLAERGYRVAANDLDAPEGTL
                     DELRSTGAEALSVPGDVSDETVVRGMVEAVVDEFGRIDVVVNNAGISAIVPAEETTLE
                     DWDRTLAVNLTGPFLMCREFGKVMLEGGAGSIINVSSVAGLLGISDRAAYNA"
     gene            complement(476..1366)
                     /locus_tag="VHM69_13740"
     CDS             complement(476..1366)
                     /locus_tag="VHM69_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2741502.1"
                     /translation="MAEQGRDRWAAWILERRHGGDPEDLKATMDFLIPVRDRVLGNAA
                     LTGGETLLDVGAGDGLIAFGALELVGERGRVIFSDISQDLLDHCRSVALQMGVLGRCE
                     FVRAPADDLSVLEDTSVNAVTTRSVLIYVKDKRRAFEEFHRVLKPGGRLSIFEPINNF
                     TYPEPPHLFGGSDVRAVADLAEKVKAVYASRQLPDECPMLDFDERDLFDLAERAGFEE
                     IHLSYEAKLIPGNLGDGARDWETALRSAPNPLAPTLGEAMDEALTPDEAERFTAHLRP
                     LVEKNENTARNAVAYLWTVR"
     gene            complement(1371..2327)
                     /locus_tag="VHM69_13745"
     CDS             complement(1371..2327)
                     /locus_tag="VHM69_13745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX2741503.1"
                     /translation="MPEKVLVTREIPEAGLRLLEDFDLTVLSEAPPERDELLEAARGA
                     AGVLSTVTEKIDGEFMDAAGEGLKVVANLAVGYDNVDVEAANVRGVIVTNTPGVLDET
                     TADVAFMLLLAAARRLGESERVLRAGKWEWWGPKLFMGPDVWGKKLGIIGLGRIGQAV
                     ARRAKGFEMDILYHNRSRKVEAERELGARYMDLGELLETADFVTIHTPLTDETQHLIG
                     RAELHKMKSEAVLVNTSRGPVVDEGALADALAEGRIFAAGLDVYEEEPKVHPKLLELE
                     NVVLAPHIGSGSIETRNKMAALAAENLAAVLRGEEPKTPVNG"
     gene            complement(2350..>2748)
                     /locus_tag="VHM69_13750"
     CDS             complement(2350..>2748)
                     /locus_tag="VHM69_13750"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2741504.1"
                     /translation="PLVVLAGKEYGSGSSRDWAAKGSFLLGVKAVIAESFERIHRSNL
                     IGMGVLPLQYTQGENAESLGLSGHESYDISGLADLEPGKELTVRATGDDGETREFNVK
                     ARVDSPVEVEYLRNGGILHTVLRQMLKEDS"
BASE COUNT          437 a          910 c          856 g          545 t
ORIGIN      
        1 cgcgttgtac gccgcgcggt ccgagatccc gagcagcccc gccacggaac tgacgttgat
       61 gatgctcccc gcgcctcctt ccagcatcac tttgccgaac tccctgcaca tcaggaacgg
      121 gccggtcagg ttgacggcga gggtcctgtc ccaatcttcg agcgtcgtct cctcagcggg
      181 cacgatggcg ctgatacctg cgttgttgac aaccacgtcg atacgcccga actcgtccac
      241 cacagcctcg accatcccgc gcaccaccgt ctcgtccgag acgtcgccgg ggacggatag
      301 cgcttcggcc cctgtggagc gtagctcatc gagggtgccc tccggggcat cgaggtcgtt
      361 ggcggctacc ctgtagcctc gttcggcgag ggtcagcgag acctgtctac ctatgccgcg
      421 ggatgctcct gtaacgatgg ctacgcgatc gtctgtactc aacgggactc ctctctcatc
      481 tgacggtcca gaggtatgcg acggcattgc gtgctgtatt ctcgttcttc tctaccagag
      541 ggcgaaggtg cgccgtgaag cgttccgctt catccggtgt cagggcttcg tccatcgcct
      601 ctccgagcgt tggtgccagc gggttgggcg cgctcctgag ggcggtctcc cagtcgcgtg
      661 cgccgtcccc gaggttgccg ggaatcagtt tggcctcgta gctcaggtgg atctcctcga
      721 agccggcccg ttcggcgagg tcgaaaaggt cccgctcgtc gaagtcgagc atcggacact
      781 cgtccgggag ctggcgagaa gcgtagaccg cctttacctt ctcggcgagg tcagcaacag
      841 ctctcacgtc ggaaccgccg aagaggtgcg gcggctcggg ataggtgaag ttgttgatcg
      901 gctcgaagat cgaaagccga cctcctggtt tgaggacgcg gtggaactcc tcgaaggccc
      961 gccgtttgtc cttaacgtag atcaaaaccg agcgcgtagt caccgcgttt accgatgtgt
     1021 cctccagcac agagagatcg tcagccggtg cgcgcacgaa ctcgcaccgg ccgagcacgc
     1081 ccatctgaag cgcgaccgag cgacagtgat cgagcaggtc ctgggagatg tcgctgaaga
     1141 tgacacgtcc cctttccccg acgagttcga gtgcaccgaa ggctatgaga ccgtcgccgg
     1201 ccccgacgtc gagcagcgtc tcgccgcctg tgagcgcggc gttcccgagt accctgtcgc
     1261 gcacgggtat caggaagtcc atagtggctt tcaggtcttc cggatctccc ccgtggcgcc
     1321 gctccaggat ccaggcagcc cacctgtctc gaccctgctc cgccatgcgg ttatccgttg
     1381 accggcgtct tcggctcctc gccgcgcagc acggctgcaa ggttctccgc ggctagggcc
     1441 gccatcttgt tgcgtgtctc gatggagccg ctgccgatgt gcggggcgag gacgacgttc
     1501 tcgagctcga gcagcttcgg atggaccttc ggctcctcct cgtatacgtc gagccccgcg
     1561 gcgaagatac gcccttccgc cagggcgtct gccagagcgc cctcgtccac gacggggccg
     1621 cgcgaggtgt tgacgaggac ggcttcggac ttcatcttgt ggagttcggc tcgtccgatg
     1681 aggtgttggg tctcgtcagt tagcggggtg tgtatcgtga cgaagtccgc ggtttcgagc
     1741 agctcgccga gatccatata acgggctccg agctctcttt ccgcttccac tttccgagag
     1801 cggttgtggt agaggatgtc catttcgaag cccttcgcgc ggcgggcgac ggcctgcccg
     1861 atcctgccga gccctattat gccaagtttc ttgccccaca cgtcagggcc catgaacagc
     1921 ttcggtcccc accactccca tttcccggcc cgcagaacgc gctcgctctc cccaagacgg
     1981 cgggccgcag ccaacagtag catgaacgcc acgtccgcgg tcgtctcgtc gaggacgccg
     2041 ggcgtgttgg tgacgatcac gccgcgcacg tttgccgcct ccacgtcaac gttgtcgtaa
     2101 ccgaccgcga ggttggctac gaccttcaac ccttctcctg cagcgtccat gaactcgccg
     2161 tcgatcttct ccgtaacggt ggagagaacc cctgccgccc cgcgggcggc ttcgagaagc
     2221 tcatcgcgct ccggtggggc ctcggagagg acggtcaagt cgaagtcctc gaggagacgg
     2281 agcccggcct cggggatctc gcgcgtcacg agcacctttt cgggcacggg ctcctcctat
     2341 cttagcgttc tagctgtcct ctttgagcat ctggcgcagc acggtgtgca ggatgccgcc
     2401 gttacgcagg tactcgacct ctacgggcga gtccacgcgc gcctttacgt tgaactccct
     2461 ggtctcgccg tcgtcgcccg tagctctaac tgtcaactcc ttgccaggct ccaggtcggc
     2521 gaggcctgag atgtcgtagg actcgtggcc gctcaggccg agggactctg cgttctcgcc
     2581 ttgtgtgtac tggagcggga ggacgcccat gccgatcagg ttcgagcgat ggatgcgctc
     2641 gaagctctcg gcgatgacgg ccttgacccc gaggaggaat gagcccttcg cggcccagtc
     2701 acgcgaggag cctgagccgt actccttgcc agcgaggacg acgagcgg
//
LOCUS       DATAIR010000576         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_340193, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000576 DATAIR010000000
VERSION     DATAIR010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340193"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VHM69_13755"
     CDS             complement(<1..1262)
                     /locus_tag="VHM69_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2741505.1"
                     /translation="MMMADVKKLEVPEPLSRLGEAFRGAGHELYLVGGYVRDSLLGGA
                     GPDLDATTGAHPREIKRLLGARAEHLWTVGERFGTIGAMVDGYAVEVTTYRTDLYTEG
                     SRHPEVRFGESLEEDLARRDFTINAVAADALTGEILDPFEGRKDLEAGVIRPVGDPLD
                     RMRDDPLRMLRAVRFETTLSNPQKPFAMTPDLEEAISNNAHWLESISAERIRGEFEKI
                     LLSENVASGLRTLVRLGLMPYIVPEFMETVDVEQEAEFHHKDVFEHTLIVVSSIEPGP
                     DLTVRDFSILRKAAFFHDIGKPRTLVYEHRCTYCGAKSTQKTPEEGECERCGGRTIPK
                     KIHFYGHENVGAAIARRAMQRLAYPRDDIDAVAHLVANHMRPYGYAASRDPWSDAAVR
                     RFVRDTYLARGDRELANVDMLLRLARAD"
     gene            complement(1304..1627)
                     /locus_tag="VHM69_13760"
     CDS             complement(1304..1627)
                     /locus_tag="VHM69_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2741506.1"
                     /translation="MDEHAEGYSVIAVRGEMDLHTAPKFQYAIERAAEKEGVGAVVVD
                     MGDVAFMDSTALSALMRSKDALDQQGVSLRLAAPSNAVERIFSVTGFQDYFDIFSSRE
                     AAIPA"
BASE COUNT          323 a          600 c          572 g          376 t
ORIGIN      
        1 atgtcggccc tggcgagcct taggagcatg tccacgtttg cgagctccct gtcgccgcgc
       61 gccaggtacg tgtccctgac aaagcgtctg accgccgcgt cgctccaagg gtcgcggctc
      121 gcggcgtatc cgtaggggcg catgtggttc gcgacgaggt gggcgacggc gtctatgtcg
      181 tccctcgggt atgcgaggcg ttgcatcgcc cggcgcgcaa tggccgcccc aacgttctcg
      241 tggccgtaga agtggatctt cttcgggatc gtacgccccc cgcaacgctc acactcgccc
      301 tcttcaggcg tcttctgcgt gctcttcgcg ccgcagtagg tgcagcggtg ctcgtagacc
      361 agcgtccgcg gcttgccgat gtcgtggaag aaggcagcct tgcgcaggat ggagaagtct
      421 ctcactgtca agtcgggacc cggctcgata ctggatacca cgatcagggt gtgctcgaag
      481 acgtccttgt ggtggaactc ggcctcctgt tctacgtcga ccgtctccat gaactcgggc
      541 acgatgtagg gcatgagccc gaggcgaacg agcgtcctca gacccgacgc gacgttctcg
      601 gagagcagga tcttctcgaa ctcgcccctg atgcgctctg cggagatgct ttccagccag
      661 tgggcgttgt tgcttatggc ctcttcgagg tccggcgtca tcgcgaaagg cttctgtgga
      721 ttactgaggg tcgtctcgaa gcggacggcc ctcagcatcc tcaaggggtc gtcgcgcatc
      781 ctgtcgagtg ggtctccgac gggacgaatg acaccggctt cgaggtcctt cctgccttcg
      841 aaagggtcca gtatctcacc cgtcagcgca tcggctgcga cggcgttgat cgtgaagtcc
      901 ctcctcgcga ggtcctcttc gaggctctcc ccgaacctta cctcgggatg gcgactccct
      961 tccgtataca ggtcggtcct gtaggtcgtg acctcgaccg cgtaaccgtc taccatcgcc
     1021 ccgatggtcc cgaagcgttc cccgacggtc cacaggtgct cggcgcgcgc cccgaggaga
     1081 cgtttgatct ccctcggatg ggcgcccgtc gtcgcatcca ggtcaggccc ggccccgccg
     1141 agcaggctgt cccgcacgta cccacccacc aggtacagct cgtgccccgc ccccctgaaa
     1201 gcctcgccga ggcgcgaaag gggctccgga acctcaagct tctttacgtc agccatcatc
     1261 atgcccagat tctagtatct atgcggccca agtctaggag acgtcaggct gggatggcgg
     1321 cttcgcggga ggagaagata tcgaagtagt cctggaagcc ggtcacggag aagatgcgtt
     1381 cgacggcatt ggagggggcg gcgaggcgaa gggagacccc ctgttgatcg agtgcgtcct
     1441 tggagcgcat gagggccgag agggctgtgg agtccatgaa agccacgtcg cccatgtcga
     1501 cgacgaccgc gccgacaccc tccttctcgg cggcccgctc tatcgcgtac tggaacttcg
     1561 gcgcggtatg caggtccatc tcaccccgca ccgctataac ggagtatccc tctgcatgct
     1621 cgtctatgct aacctcgaag tccgtgccag ctccctccta tctacggccc gttcgactcg
     1681 gccaatggat attaacgcag ttcatgcgcc cggtaaacag gcggaattgt acgggcatgc
     1741 aatgatatct gctaatattc gggcgtgatg gaaggtagaa ccactgagcg ggtcgccgtc
     1801 ttcgtcgacg gggcgaacct ctaccactcg atcaagaact actacaaggg cgttctcgac
     1861 tactccaggc t
//
LOCUS       DATAIR010000577         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_248527, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000577 DATAIR010000000
VERSION     DATAIR010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248527"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="lgt"
                     /locus_tag="VHM69_13765"
     CDS             <1..738
                     /gene="lgt"
                     /locus_tag="VHM69_13765"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX2741507.1"
                     /translation="ALGIIVATWLTGRELERKGYDGTLALDSLFFIVPLGFVGARAYH
                     VATDYDLYASDPFPGVFAVWNGGLGIYGGVVGGFVGLWIFARIRDISPWVLADAIAPG
                     LILAQAIGRWGNYFNQELFGRPSDLPWAIRIAPENRPVEFSDVGAFHPTFLYEAIWNV
                     FVCLILLWVARRFADRLKDGDIFLLYVSLYSFGRFFVEALRVDPAFLIGGTIRGNLFV
                     SSLLALGFAMILLLRHSRSGRKRPDSR"
     gene            complement(740..>2155)
                     /locus_tag="VHM69_13770"
     CDS             complement(740..>2155)
                     /locus_tag="VHM69_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX2741508.1"
                     /translation="GKRDRTRHPGSLAASRWVAIGACGAALGFIFLSLPVDAGGASKS
                     FSRDAVVNVVMSEVDHARIRELADNANVTPIEPPLGTHLQQTAQTEQKNVVLIHLESV
                     RARSTTPYNPRIDTTPYLNELSRQSLMADNAYTIVPHTSKAITAINCGIDPHLVRKIT
                     EAEPGGVPARCLPELLKEQGYDSVWFSSATEHFEDRRDLVKNFGYAHFKPVESMDKEG
                     FEKSNYFGYEDDIMLQPSKYWLEKHGDRPFMATYLGVTGHHDYRPIERYGLEHYAEDS
                     ALNHYQNEVRYLDFFVKNVIDQYKALGLYDNTIFVIYGDHGEGFGAHDLYQHDNTIYQ
                     EGLKIPLIIHEPGHFQEGKRVETLTDQVDIMPTLLDLLGYKVTGGDYMGRSLLDPPDG
                     DRTLFFSCFDDYKCLASLKGTEKYIYFFGNQEEEVYDLASDPFERDNIADQQSKKELK
                     KKRLQLLAWYSRVNGLYEDKK"
BASE COUNT          344 a          640 c          670 g          501 t
ORIGIN      
        1 gcgctcggca tcatcgtcgc gacctggctt acggggaggg agctggagcg caagggctac
       61 gacggcactc tggccctcga ctcgctgttc ttcatcgtgc ctcttggctt cgtcggcgcc
      121 agggcttacc acgtggcgac cgactacgat ctctacgcaa gcgacccctt tcccggtgtg
      181 ttcgcggtct ggaacggggg gctcgggatc tacggcgggg tcgtcggggg gtttgtaggg
      241 ctctggatct tcgcccgcat cagggatatc agcccgtggg tcctcgccga cgcgatagcg
      301 ccgggcttga tcctggccca ggcgatcggg cgctggggca actacttcaa ccaggaactc
      361 ttcggaagac cttctgacct gccgtgggcc atacggattg cgccggagaa ccggccagtc
      421 gaattctccg acgtcggcgc ttttcacccg actttcctgt acgaggcgat ctggaacgtc
      481 ttcgtctgcc tgatcctgct ctgggtggcc cgccgtttcg ccgaccgtct gaaggacggt
      541 gatattttcc tgctttacgt cagcctctac tctttcggac gcttcttcgt cgaggcgctg
      601 cgcgtcgacc ccgcgttcct gatcggcgga actatccgcg ggaacctctt cgtgagcagt
      661 ctcctggcac tcggcttcgc catgattctg ctcctcaggc attcccggtc agggcgaaaa
      721 cgtcccgact cccgctgagc tacttcttat cctcgtacag gccgttgacc ctcgagtacc
      781 acgcgagcag ttgtaaccgt ttcttcttca gctctttctt gctttgctgg tcggcgatgt
      841 tgtccctctc gaaggggtct gacgccagat cgtacacttc ctcctcctgg ttgccgaaga
      901 agtagatgta cttctcggtc cctttcaggc tggcgaggca cttgtagtcg tcgaagcagc
      961 tgaagaacag ggtcctgtct ccgtccggag ggtcgaggag ggatctcccc atgtaatcgc
     1021 cccccgtgac tttgtagccg agcagatcta agagggtggg catgatgtcg acctggtccg
     1081 tgagggtctc gacgcgcttc ccttcctgga aatgtcctgg ctcgtggatg atgagcggta
     1141 tcttgagccc ctcctggtag atggtgttat cgtgctggta gaggtcgtgt gccccgaacc
     1201 cttcgccgtg gtcgccgtag atcacgaaga tagtgttgtc gtagagcccg agcgccttgt
     1261 actggtctat gacgttcttt acgaagaagt cgaggtagcg gacctcgttc tggtaatggt
     1321 tgagcgccga atcctcggcg taatgctcga gcccgtaacg ttcgatggga cgatagtcgt
     1381 ggtggcccgt gacgccgagg tacgtagcca tgaatggccg gtcgccgtgc ttctcgagcc
     1441 agtacttgct cggttggagc atgatgtcgt cctcgtagcc gaagtagttg gatttctcga
     1501 aaccttcctt gtccatcgac tcgacgggct tgaagtgggc gtagccgaag ttcttgacga
     1561 ggtccctgcg gtcctcgaag tgctcggtcg ccgaggagaa ccagacggag tcatacccct
     1621 gctccttcaa caactctggc aggcatcggg ccgggacgcc gcctggctcg gcctcggtga
     1681 tcttcctcac caggtgcggg tcgatgccgc agttgatggc ggttatggcc ttggaggtgt
     1741 gtggcacgat cgtatacgcg ttatcggcca tgaggctctg ccgcgagagc tcgttcaggt
     1801 agggcgtcgt atcgatgcgc gggttgtaag gcgtcgtgga ccgcgctcgg acggactcca
     1861 ggtggatcag gactacgttt ttctgctcgg tctgggcagt ctgctgcaga tgggtgccta
     1921 atggaggctc tatgggggtg acgttggcgt tatcagcaag ctctctgatc ctcgcgtggt
     1981 ccacctccga catgaccacg ttgaccacag cgtccctgga gaaggacttg ctggccccac
     2041 ccgcgtcaac gggcagcgag aggaagatga accccagcgc agcgccacat gccccgattg
     2101 cgacccacct ggacgcggcc agagaacccg gatgccgcgt cctgtcacgc tttcc
//
LOCUS       DATAIR010000578         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_131825, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000578 DATAIR010000000
VERSION     DATAIR010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131825"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="hemB"
                     /locus_tag="VHM69_13775"
     CDS             <1..1006
                     /gene="hemB"
                     /locus_tag="VHM69_13775"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX2741509.1"
                     /translation="PTRQGEVSRMAFPRQRLRRTRRTAALRGLVRETRLTPEDFVYPI
                     FVTAGEDVRNPVASMPGIFQLSISHAVAEAEMAHDLGIPAVLLFGIPDQKDEAATGAY
                     DPEGIVQLATRAIKEAAPDLLVITDVCLCEYMSHGHCGVVEKDTGEVLNDVSLELLAR
                     TAASQAEAGADVVAPSDMMDGRVAAIRAELDNEGFENTPIMAYAAKYASAFYGPFRDA
                     ADSAPQFGDRRSYQMDPANAREALVETELDIEEGADIVMVKPALPYLDVLRRVRETTD
                     LPVAAYNVSGEYAMVKAASQNGWLDEERVVLEALTGIKRAGADIIISYHAPDVAKWLA
                     "
     gene            complement(1036..1266)
                     /locus_tag="VHM69_13780"
     CDS             complement(1036..1266)
                     /locus_tag="VHM69_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEX2741510.1"
                     /translation="MGKPIELYTTAGCPYSEAAREDLEWRGVDFVEYDVEKDFKARER
                     MLGLTGGNRTVPVIFEEGSPVQIGWMGRGCAV"
     gene            1391..1729
                     /locus_tag="VHM69_13785"
     CDS             1391..1729
                     /locus_tag="VHM69_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX2741511.1"
                     /translation="MHELSIAESVVSIASRQADGRRVTKVRMKVGHLRQVVPSALAFG
                     FELLAEGTPVEGAELEMEQVPAEGTCRVCGAESRLDSFPLQCLGCGGFDLEVLKGEEL
                     LVESLELEEA"
     gene            1732..2400
                     /gene="hypB"
                     /locus_tag="VHM69_13790"
     CDS             1732..2400
                     /gene="hypB"
                     /locus_tag="VHM69_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX2741512.1"
                     /translation="MHRTAVKKVVMENVLDANDTLALANREMFARAGTYTINMMSSPG
                     AGKTAILERTLEQMRGRLRLGVLEGDVQTTLDADRLARFHIPLVQVNTDPGFGGECHL
                     DANMVRSGLAELPLDDIDVLIIENVGNLVCPAEFRVGEDVRVMVYSVTEGEEKPLKYP
                     LMFRSADLVLVNKVDLLEYLDFDLEQFLGNLDAVNPGVRRILTSAKTGQGLDEWCGWL
                     GQRP"
     gene            2712..>3518
                     /locus_tag="VHM69_13795"
     CDS             2712..>3518
                     /locus_tag="VHM69_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741513.1"
                     /translation="MAETQSTASGVEEVHILWISEGMSCDGDSVSITAATLPSIEDVL
                     LGNIPGLPKVHLHNKVLHPSLGGEDFLQPFRQAVDDELDAPFVLVIEGSIPNENINGD
                     GYWTSFGNDPATGDPLTLNWWIDRLAPKAFAVVAAGTCATYGGIHAMAGNPTGCMGLA
                     DYLGWNFRSVAGIPIVNVPGCPVQPDNFMETVTWLLYQAAGLSPMIPLDEQLRPTWLF
                     GKTVHEGCDRAGYYEQGDFALDYNSPKCLVKVGCWGPVVNCNVAKRGWMDG"
BASE COUNT          676 a         1052 c         1157 g          633 t
ORIGIN      
        1 gccgacccgg caaggagagg tgagtaggat ggcgttcccg cggcaaagac ttcgaaggac
       61 caggcgcacg gcggctcttc gcgggctcgt gcgcgagacc cggctcacgc cggaggactt
      121 cgtgtatccg atcttcgtga ctgcggggga ggacgtaagg aaccccgttg cctcgatgcc
      181 aggcatcttc cagctctcca taagccacgc ggtcgcggag gctgaaatgg cgcacgacct
      241 cggaatacct gccgtactgt tgttcggaat cccggatcag aaggacgagg ctgccacggg
      301 cgcctacgac ccggaaggca tcgtccagct cgccacccgc gccataaagg aagcggcgcc
      361 tgatctgctc gtgataacag acgtgtgcct gtgcgagtac atgagccacg gccactgcgg
      421 ggtcgtcgag aaggatacgg gcgaggtcct aaacgacgtt agcctggagc ttcttgcgcg
      481 aaccgcggcg tcccaggccg aggccggggc cgacgtcgtc gctccctccg acatgatgga
      541 cgggcgggtg gcggcgatcc gggccgaact ggacaacgaa ggcttcgaga atactcccat
      601 tatggcctac gcggccaagt acgcctcggc gttctacggc ccgttccgcg acgccgccga
      661 cagcgcgccg cagttcggcg acaggcgcag ctaccagatg gatcctgcca acgcccgcga
      721 ggcgctcgtc gagacggagt tggacatcga ggagggcgcc gatatcgtga tggtcaagcc
      781 cgccctcccg tacctggacg tactccgccg tgtgcgcgaa acgaccgatc tacccgtcgc
      841 cgcatacaac gtgagcggtg agtacgcgat ggtcaaggcc gcatcccaga acggctggct
      901 cgacgaggag cgggtcgtcc tcgaggctct gacaggcatc aagcgcgcgg gtgctgacat
      961 cattatcagc taccacgccc ccgacgtggc aaagtggcta gcctgacgta agggcggttc
     1021 aggaaccgag caggatcaga ccgcacaccc cctgcccatc cagccgatct gaacgggact
     1081 gccctcctcg aagattactg gtacggtacg gttgccaccg gtcagcccga gcatgcgctc
     1141 gcgggctttg aagtccttct ccacgtcgta ctcgacgaag tcaaccccgc gccactccag
     1201 gtcctcacgg gcggcctcgc tgtagggaca gccggccgtg gtatagagct ctatcggctt
     1261 gcccatgtat cctccttacg cggcgtacgc cccctgattc tatccctcga cgaagctctc
     1321 cggcgttgtt gccccgattg ttatggtcta cgatggggtc gcggcttctt tacgccgtga
     1381 ggagtagcgc ttgcacgaac tctctatagc ggagtctgtg gtcagtatcg ccagccggca
     1441 ggcagatggg cggcgggtta ctaaggtgcg gatgaaggtg ggtcatctgc gccaggtcgt
     1501 gccttcggcg ctggctttcg gtttcgagct ccttgcggaa ggaacgcccg tcgagggtgc
     1561 cgaactggag atggagcagg tccccgccga aggaacgtgc cgcgtgtgcg gggctgagag
     1621 ccgcctggat agcttcccgc tacagtgcct ggggtgtgga ggcttcgacc tggaggtcct
     1681 caaaggggaa gagttgctgg tcgagtcact cgaactggag gaggcgtagg gatgcaccgc
     1741 acggcggtca agaaagtcgt catggagaac gttctggacg ccaacgacac gctcgccctg
     1801 gcgaacaggg agatgttcgc cagggccgga acgtacacga tcaacatgat gagctcgccg
     1861 ggggccggca agacggcgat acttgagcgc accctggagc agatgcgcgg caggctacgc
     1921 ctgggcgtac tcgaaggcga cgtgcagacc acgctcgacg cggaccgcct tgcccgcttt
     1981 cacatcccgc tcgtgcaggt gaataccgac cccgggttcg gcggcgagtg ccacctggac
     2041 gccaacatgg tgagatccgg cctcgccgaa ctaccgctcg acgacataga cgtactcatt
     2101 atcgagaacg tggggaacct ggtgtgtccg gccgagttca gggtgggcga ggacgtcagg
     2161 gtcatggtct actccgtcac ggaaggtgag gagaagcctc tcaagtaccc gctcatgttc
     2221 cggtctgctg acctcgtact cgtcaataag gtcgacctgc tcgagtatct tgacttcgac
     2281 ctggagcagt tcctggggaa cctcgacgcg gtcaaccccg gagtcaggcg catcctcacc
     2341 agcgcgaaga caggtcaggg cctcgatgag tggtgcggct ggctcgggca acgtccctga
     2401 gatagggatc gtatccaagt ttacaaccgg tttgaacgct ggtatcctgc ctgtcctact
     2461 tgtttgtggg tgatgggtag ttgggaaaag cggcggttcc gggattcggg aagatggctg
     2521 gatctctggt cattccttag gggtagggag tgtaccggtg gagcgctcga caaagggaag
     2581 ctcatatggg gtgcaggccc ggcgacatgg tccggaccac gacaggggtt gaagttcatc
     2641 tattcgtacc cttctgagga acgctctttt taagaatcgg cggtcgtatg gcgcaagggg
     2701 aaagcagaat cgtggccgag actcaaagca cagccagcgg ggtagaggag gttcacatac
     2761 tgtggatctc ggagggcatg agttgcgacg gcgactcggt ctccatcacg gcggccactc
     2821 tgccaagtat cgaggacgta ttgctcggga acataccggg gctgcccaag gtacatctgc
     2881 acaacaaggt tctccatccc tctctcgggg gggaagactt cctgcaaccc ttcaggcagg
     2941 ctgtggacga cgagcttgac gcgccattcg tgctcgtgat cgagggctcc atccccaacg
     3001 agaacatcaa cggcgacggg tactggacct ccttcggcaa cgaccccgcg acaggcgatc
     3061 cgctgacgct caactggtgg atcgaccgtc tcgccccgaa ggcttttgca gtggtggcgg
     3121 cagggacgtg cgcgacctac ggcggcatcc acgcgatggc tggcaacccg acggggtgca
     3181 tggggctcgc agattacctc gggtggaact tccgctccgt ggcaggtatc ccgatcgtga
     3241 acgtgccagg gtgccccgtc cagcccgaca acttcatgga gaccgtcacg tggttgctgt
     3301 atcaggccgc cggtctatcc cccatgatcc cgctcgacga gcaactgagg ccgacctggc
     3361 tgttcgggaa gaccgtgcac gagggttgcg acagggcagg gtactacgag cagggcgact
     3421 tcgcactcga ctacaactcg cccaagtgcc tggtcaaagt cggctgctgg ggacccgtcg
     3481 tcaactgcaa cgtggcgaag agaggctgga tggacggc
//
LOCUS       DATAIR010000579         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_408950, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000579 DATAIR010000000
VERSION     DATAIR010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408950"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VHM69_13800"
     CDS             complement(<1..940)
                     /locus_tag="VHM69_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2741514.1"
                     /translation="MLQQVRSARVSLGATVALGLLAAAATIAQLVLLSKVVDRVFLGG
                     QDLGEVRDLLLLLLGASFLRSGLLWAREVAAQRGAVRVKSELRERLFAHVLRLGPSYT
                     RGERTGELTTTATEGVEKLDAYVGRYLPQTFLSALVPLMIAGYVFPLDPSSAVLLLVT
                     APVILILMVLVGSYAEEHTRRQWKALSRMGASFLDAIQGLTTLKVFGRSADESENVAA
                     ASEEFRKRTMKVLRYAFLSGFVLEFMTAAAIGLVAVTLGLRVISGNMPFEAAFLVLLL
                     APEFYKPLRELGVHRHAGMEGSAAAERIFEILSTPVL"
     gene            complement(1016..2056)
                     /gene="cydB"
                     /locus_tag="VHM69_13805"
     CDS             complement(1016..2056)
                     /gene="cydB"
                     /locus_tag="VHM69_13805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2741515.1"
                     /translation="METAWFIFVALMIAVYVLLDGFDIGAGAIHLFAARDDRERRTII
                     RAIGPVWDGNEVWLIAAGGTLFFAFPVLYASSFSGFYLPLIIVLWLLMIRGLSIELRG
                     HIEDPLWASFWDAMFFIGSTLLAVFYGAALGNVVRGVPLNENGYFFQPLWTDFNPFSQ
                     TPGILDWYTVLIGLLALTTLVVHGANFVAVKTEGGLNGRSRRISRLFTYATAALTLLA
                     TPATFWISPEMLRSFNERPYGYVLPLVAIAGLVGAVLFNSRDNERAAFLSSGAYIIGM
                     LTSTVFAIYPNVLPAIDPSNSLTIHNASASQYGMAVGLVWWSIGMVLAAVYFIVIYRL
                     FRGKVDLEDEGY"
     gene            complement(2070..>2439)
                     /locus_tag="VHM69_13810"
     CDS             complement(2070..>2439)
                     /locus_tag="VHM69_13810"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2741516.1"
                     /translation="GLGTIFIAIMVLAAFLLWRGRLYDARWMLWILMLAIPFPFIANT
                     AGWFTAELGRQPWLAYGIFRTSEGVSPLVSSGSVLFTLIGFAGMYLVMGLLYIVLMVR
                     EVAHGPEGEETVESAEGVTV"
BASE COUNT          526 a          763 c          770 g          380 t
ORIGIN      
        1 caagcacagg ggtggacagg atctcgaaga tgcgctccgc tgcggcgctc ccctccatgc
       61 cggcgtggcg gtggacgcct agctcgcgca gaggtttgta gaactcgggc gccaggagca
      121 gcaccaggaa ggcagcctcg aagggcatgt tcccggagat cacacgcaac ccgagcgtga
      181 cggcgacgag acctatggcc gccgcggtca tgaactcgag tacgaacccg gagaggaagg
      241 cgtagcgcaa caccttcatc gtgcgcttcc tgaactcctc gctggccgcc gccacattct
      301 cgctctcgtc ggcgctcctg ccgaagacct tgagggttgt gaggccctga atggcgtcca
      361 ggaagctcgc tcccatgcgc gagagtgcct tccactggcg gcgggtgtgc tcctccgcgt
      421 agcttccgac cagcaccatc aatatcagaa tcacaggcgc cgtaacgagc agcaggacgg
      481 cgctcgaagg gtccagtgga aacacgtacc cggctatcat caggggaacg agcgcgctga
      541 ggaacgtctg cggcaagtag cggccaacgt acgcgtcgag cttctcgaca ccctcggtcg
      601 cagtcgtcgt gagctcgccg gtacgctccc ccctcgtata cgacggaccg agacgcagga
      661 cgtgcgcgaa caaccgctcc ctgagctcgg acttcacccg cacagccccc cgctgggcgg
      721 cgacctcgcg ggcccacaag agaccagaac gcaggaacga ggcaccgagc aagaggagga
      781 gtagatctct cacctccccg agatcctgcc ctcccaggaa caccctgtcc acgaccttgc
      841 tcaacaagac cagctgtgcg atcgtcgcag ctgcggccag aagccccagc gccacggtcg
      901 cccccagaga gacccgcgct gaccgaacct gctgcagcaa ctccctattc atcgacccgt
      961 ccaagcaaag gtaaagtcca agaagctgac tgctgaaagc tgatagctga ccgctctaat
     1021 acccttcgtc ttccagatct accttgccgc ggaagagacg gtagatgacg atgaagtaga
     1081 cagcggcgag caccatgcct atgctccacc agacgaggcc gaccgccatt ccgtactggg
     1141 aggctgaggc gttgtggatc gtgaggctgt tcgacgggtc gatggcgggg aggacgttcg
     1201 ggtagatggc gaagacggtg ctcgtcagca tcccgattat gtaggcgccg gacgagagga
     1261 acgccgccct ttcgttgtcc ctggagttga agagaaccgc gccgaccagc ccggctatgg
     1321 cgacgagcgg gaggacgtat ccgtatggcc gctcgttgaa gctccggagc atctcagggc
     1381 tgatccagaa ggtggccggg gtggcgagca gggtcagtgc agcggtcgcg taggtgaaga
     1441 ggcgtgagat cctgcgtgag cggccgttca gcccgccctc ggtttttacg gcgacgaagt
     1501 tcgccccgtg cacgacgagc gtggtgaggg ccagcagccc gatgaggacc gtgtaccagt
     1561 ccaggatgcc tggagtctgg gagaagggat tgaagtccgt ccacaacggc tggaagaagt
     1621 agccattctc gttcagcgga acgccgcgca cgacgttgcc gagggctgcg ccgtagaaga
     1681 cggcgagcag cgtgctcccg ataaagaaca tcgcgtccca gaaagaggcc cacagcggat
     1741 cttctatgtg tccgcgcagc tctatggaga ggccccggat catgagcagc cagagcacga
     1801 tgatgagcgg caaatagaag ccggagaagc tggaggcgta cagtacggga aacgcgaaga
     1861 acaacgtacc tccggctgcg atcagccaga cttcatttcc gtcccacaca gggccgatgg
     1921 cgcgtatgat cgtgcgccgc tccctgtcgt ctctggcggc gaaaaggtgt atggctcctg
     1981 cgccgatgtc gaagccgtcc aggaggacgt aaacggcgat catcaacgcg acaaagataa
     2041 accaggccgt ctccacgatc cctcctcttc tagactgtca cgccttcagc cgattccaca
     2101 gtctcctcgc cttcggggcc gtgggcgacc tcgcgcacca tgagcacgat atagaggagg
     2161 cccatgacga gatacatccc cgcgaaccct atgagcgtga agagcacgct gcccgaggag
     2221 acgagcgggg agacgccctc cgacgttctg aagatgccgt aggcgagcca gggctgtcgc
     2281 ccgagctccg ctgtgaacca ccctgcggtg ttcgcgatga acggaaaggg gatggcgagc
     2341 atcaggatcc agagcatcca gcgagcatcg tacagcctgc cacgccacag caggaacgcc
     2401 gccaggacca tgatggctat gaagatagtc ccgaggccg
//
LOCUS       DATAIR010000580         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_39299, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000580 DATAIR010000000
VERSION     DATAIR010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39299"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1669
                     /locus_tag="VHM69_13815"
     CDS             176..1669
                     /locus_tag="VHM69_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX2741517.1"
                     /translation="MSLSLFGIDALPVEVEVDVSPGLPGFSIVGLPDAAVQEARERVR
                     VAISNSGYKFPTKRVLVNLAPANLRKEGPAFDLPIALGILAASGALAPGGLERAGVAG
                     ELSLDGGVRGIRGALSMAEGARREGLRRLVVPERDAPEAAVIGGVEVYGMRTLRAAVE
                     FLGDPTRVSPAEAAPEDGRDAAGRADDFADVAGQRYAKRALEVAAAGGHNVIMSGPPG
                     SGKTMLARRMPGILPPLTLEERIEVTKVHSASGEGNGALVTRRPFRAPHHTVSTFGLS
                     GGGSNPRPGEVSLAHHGVLFLDELPEFSRTALEALRQPLEDGHVTISRVAATLSYPAR
                     VTLLCSMNPCPCGYAGDKRRVCRCTAGQIERYQGRISGPLLDRMDIFVDVPRLTRQEL
                     REEERAEASRFVRERVIRARGIQMRRQGSPNSTLSGRLLREVCALGGDAETLFHRAMD
                     RMGLSGRGHDRVLRVARTVADLSGEDEIATEHLAEALNYRRSHILGR"
     gene            1659..2765
                     /gene="dprA"
                     /locus_tag="VHM69_13820"
     CDS             1659..2765
                     /gene="dprA"
                     /locus_tag="VHM69_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX2741518.1"
                     /translation="MDGSDRAKYLFLSLLQARVGSSLVSRLGNLAPDAVLKTPLDELS
                     ERAKLSEKVARAFDELQRGFDPDALEDRLAAKGIEAVTLVDHGYPRSLRSIPDSPPAL
                     FVDGNLPEADSVALVGSRKATATGIEAARALGMALSERGVCVVSGLALGVDAAAHEGA
                     VGAGGPTVGVLGCGIDVVYPRCNRGLFQSVRRQGALVSEYFLGEAPLAWHFPARNRII
                     AGLVGTVVVVEAPVHSGALITARHALDAGRDVWAVPGPPGVSECRGSNGLLADGAGVL
                     WDIPEFVGAIVPASEVPEVGGISDGIPPPPASLPETEAAVLSGVGLEPRGVDVVARRC
                     GVELREALAALAMLELKGFISRDGVGAYIRRAVP"
     gene            2762..>3307
                     /locus_tag="VHM69_13825"
     CDS             2762..>3307
                     /locus_tag="VHM69_13825"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2741519.1"
                     /translation="MTRRTLGALWEQYFQIRARLAEADLTCLERRLAEREARGMRDRL
                     VVNYAPLVKYVAGRTSGRMTGSLDKEDIISSGLFGLLNAVETYDPGRRTKFESYAISK
                     IRWSILDELRRLDPLSRRARLRAHQTERARDELVQKLGRAPTESELVMRLGISITEHR
                     AFLNQYARAQVRSLEARPDAQG"
BASE COUNT          559 a          984 c         1168 g          596 t
ORIGIN      
        1 agcccctaac aaacagattt gtataattta cgaaacatat ttcgtttcag tatcgtattt
       61 tagacaaaac tactatatac tggtacgact gatgcacaaa cctgggtgtt aggacgtgtg
      121 ataggggcgt catggtggca ctggggtact gcagctgatg tccgtatgcc gggcgatgag
      181 cttgagtctg ttcgggatcg acgcgcttcc ggtggaggta gaggtagacg tgagccctgg
      241 ccttcccggg ttttcgatcg tcggattacc tgacgcggcg gtccaggagg cgagggagag
      301 ggtcagggta gccatctcga acagtggcta caagttccct acgaagaggg tcctcgtcaa
      361 cctcgcgccg gcgaacctca ggaaggaagg tcctgcgttc gaccttccga tagcccttgg
      421 catcctcgct gcttcaggtg ccctggcgcc gggtgggctc gaacgggccg gagtcgcggg
      481 tgaactctcg ctcgacggtg gtgtgcgggg gatcaggggt gcgctttcga tggctgaggg
      541 tgcccggcgt gagggcctgc ggcgactcgt ggttcctgag agggacgcgc cggaggccgc
      601 tgtcatagga ggggtcgagg tatacgggat gcgcaccctt cgagcggccg ttgagttcct
      661 gggggatccc acccgagtat cgcctgcgga ggccgcgccc gaggacggac gcgacgctgc
      721 cggcagggcg gatgactttg ccgacgtggc ggggcagcgt tacgcgaagc gggctttgga
      781 agtagccgcg gcgggggggc acaacgtgat catgagcggg ccgccgggct cggggaagac
      841 gatgctggcg cgcaggatgc ctggcatcct gccaccgctc acgttggaag agcgcatcga
      901 agtaaccaaa gtccacagtg ccagcgggga gggcaacggg gcgctcgtca cgcggcggcc
      961 atttcgggcg ccacaccaca ctgtttcgac gttcgggctg tctggtggcg ggagcaaccc
     1021 gcgtcccggg gaggtatcgc tcgcgcacca cggcgtgctt ttcctcgacg agttgcccga
     1081 gttcagccgg acggcgctcg aagccctgcg ccagccgctc gaggacgggc acgtaaccat
     1141 ttcgcgggtc gcggcgacgc tcagctatcc tgcgcgtgtc acgctacttt gttccatgaa
     1201 tccatgccct tgcggatatg cgggggacaa gaggagggtc tgccggtgca cggcgggcca
     1261 gatcgaacgc taccagggac gcatctcggg acccttgctc gaccgcatgg acatattcgt
     1321 tgacgttccc cgtctcacca ggcaggaact gcgtgaagag gagcgagccg aagcgtccag
     1381 gttcgtcagg gagcgggtga tcagggcgcg tgggatccag atgcggcgcc agggttctcc
     1441 caactccacc ctctcagggc gtctcctgag ggaggtctgc gcactcgggg gcgacgcgga
     1501 gacgctcttc catcgggcga tggacaggat gggcctctcg ggaaggggcc acgacagggt
     1561 attgagggtg gccaggaccg ttgcggatct ctcgggtgag gatgagatcg caaccgagca
     1621 cctggcggag gcgctcaact accggaggtc acacatactt ggacggtagc gacagggcga
     1681 agtacctatt cctctcgctc ttgcaggcca gggtcggtag cagcctggtc tcgcgcctcg
     1741 gtaacctcgc tcccgacgcc gtcctgaaaa cgccgctcga cgagttaagt gagcgtgcga
     1801 agctctccga gaaagtcgcg cgagcgttcg acgagttgca gcgaggcttc gaccccgatg
     1861 cgctggagga ccgcctcgcg gcgaagggta tcgaggcggt cacgctcgtc gaccatggat
     1921 atccccggtc actgagaagc atacccgact ccccgcccgc gctcttcgtg gacggcaact
     1981 tgcccgaggc agattctgtc gcgctcgtag gctcgcgcaa agccaccgcg acgggtatcg
     2041 aggcggcccg cgctctcggt atggcgctct cagagcgggg cgtatgcgtg gttagcggtc
     2101 tcgcgctggg cgttgacgcc gccgcgcacg agggtgctgt cggtgcaggg gggccgacgg
     2161 taggtgtgct cgggtgcggc atagatgtcg tctaccccag gtgcaacagg ggcctgttcc
     2221 aatcggtcag gcgccagggt gccctggtat ccgagtactt cctcggcgag gcgccgcttg
     2281 cgtggcactt ccctgcgagg aaccgtatca tcgccggcct cgtagggacg gtcgtcgtcg
     2341 tcgaggctcc cgtgcacagc ggcgccctga ttaccgcccg ccacgcgctc gatgcagggc
     2401 gcgacgtctg ggccgtgccc ggccctcccg gtgtcagtga gtgcaggggc tcgaacgggc
     2461 tgctcgccga cggggcaggt gtgctctggg atatacctga gttcgttggc gctatagtgc
     2521 cggctagcga agtccctgag gtaggtggga tctccgatgg catcccgccc ccgccggcga
     2581 gtctgcctga gaccgaggcc gccgtgctct ccggggttgg cctcgagccg cgaggagtgg
     2641 acgtggtcgc gaggcgctgc ggggtggaac tgcgcgaggc gctcgccgcg ctcgccatgc
     2701 tcgagctcaa gggtttcata agtcgcgacg gtgttggcgc gtacatcagg agggcggttc
     2761 cgtgacgcgg cgtactcttg gcgctctctg ggagcagtat ttccagatac gggctcgcct
     2821 ggccgaagca gatctaacct gtctcgaacg ccggttggcc gagcgcgagg caagggggat
     2881 gcgggatcgg ctggtcgtca actatgcgcc tctcgtaaag tacgtcgccg gcagaacatc
     2941 agggcgcatg accggctcgc tggacaagga ggacatcatc tcgtcgggac tcttcgggct
     3001 cctcaatgca gtcgagacct acgatccagg gcgccgcacc aagttcgaat cttatgccat
     3061 ttccaagatc cggtggtcca tactcgacga gctgaggagg ctggatccgc tctcgcgacg
     3121 tgcgcgactc agggcgcacc agaccgagcg tgccagggac gagcttgtgc agaaactcgg
     3181 ccgcgcgccg acggagtccg agctggtcat gcgcctcggt atcagcatca ccgagcaccg
     3241 tgcgttcctg aaccagtacg cgcgggcaca ggtcaggtcg ctagaagccc gcccggatgc
     3301 tcaggga
//
LOCUS       DATAIR010000581         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_63908, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000581 DATAIR010000000
VERSION     DATAIR010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63908"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VHM69_13830"
     CDS             complement(<1..181)
                     /locus_tag="VHM69_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX2741520.1"
                     /translation="MLTAALFDFDGTLVDTTEMIHQSMRHAASSVLGRDDIPRETLLA
                     NVGQPLPRQMELIDAE"
     gene            complement(185..1003)
                     /gene="surE"
                     /locus_tag="VHM69_13835"
     CDS             complement(185..1003)
                     /gene="surE"
                     /locus_tag="VHM69_13835"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX2741521.1"
                     /translation="MRIVLTNDDGIEAPGLLAARRALEKVGDVITVAPDRNRSGVGRS
                     ISFGRSLHVEERKMADGTVGYACSGTPVDCVRLVALGLMDFEPDIVVSGINHGENLGD
                     DITYSGTVAGAMEGIVIGVPGIAVSLSINRPWHETVQEAMPLERGLHFEAAAEFTASL
                     VKVALNGLPSGRILNVNAPNLPREKLRGARVTKLGRRFYKDELVEVRDENGRVGYDIY
                     NNPPGHHEEEGTDFAAIENDEISITPVHLELTDVPGLEELCDWDVDGLLKNGRE"
     gene            1210..2196
                     /locus_tag="VHM69_13840"
     CDS             1210..2196
                     /locus_tag="VHM69_13840"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX2741522.1"
                     /translation="MDAVEWFKHRSYDYKQFSDLRALGRRKRELGLTVSAVLPCRNVA
                     DTVGGIIDEINAVNRQTGDTPLVDQILAVDADSPDGTAEVAASRGAEVYSENELMSNH
                     GGAHGKGDAMWRSLSVARGDLVMYIDADTRDFRPQLVYGILGPILEVPEIRFVKAAYR
                     RPFKSHETVEADGGGRVTELSTKPLFNLFYPELTGFVQPLAGEFVADRELFSSIPFLT
                     GYAVETGIMIDVLKMVGLDAMAQVDLGTRQNRHQPLRDLSRMSYSVLRAVARRMRQEG
                     RLNQVRDPGLPDSLFQFSDYMHAVATPDGLKLQEYVEELVERPPIKEVLRVG"
     gene            2503..2796
                     /locus_tag="VHM69_13845"
     CDS             2503..2796
                     /locus_tag="VHM69_13845"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX2741523.1"
                     /translation="MTFKPGDRVRVVPAEKPGHVRTPGYIKGKRGWIESVLGEFPNPE
                     DLAYGFSGLPERLLYKVGFRQADLWRDYDGPADDCLYADVYEHWLEPGEGGTA"
     gene            2793..3407
                     /gene="nthA"
                     /locus_tag="VHM69_13850"
     CDS             2793..3407
                     /gene="nthA"
                     /locus_tag="VHM69_13850"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX2741524.1"
                     /translation="MSDHHPHEETATSPYAARIRAIEALLVEKGVLTEPEVQDRISYM
                     EARSPANGAKLVARAWVNEDFKELLLSDGKSAASELGIDPEHPAEFVVVENTPEVHNL
                     IVCTLCSCYPRAILGRPPDWYKSFNYRSRAVSEPRAVMREFGFEIPEGVEVAVHDSSA
                     DIRYLVLPMRPPSTEGMSEEELAGLVTRDCLIGVSVPLEPVLTG"
BASE COUNT          666 a         1166 c         1113 g          628 t
ORIGIN      
        1 tttcggcgtc gatcagctcc atctggcgcg ggaggggctg gccgacgttg gcgagcagcg
       61 tctcgcgggg gatgtcgtcg cgcccgagta cggagctcgc ggcgtggcgc atggactggt
      121 ggatcatctc cgtcgtgtcg acgagcgtcc catcgaagtc gaagagggcc gcggtgagca
      181 aggcctactc gcgcccgttc ttcagcagac cgtccacgtc ccagtcgcag agctcctcca
      241 ggccgggcac atccgtcagc tcgaggtgga cgggggtgat gctgatctca tcgttctcga
      301 tggcggcgaa gtcggtgccc tcctcttcgt ggtggcctgg agggttgttg tagatgtcgt
      361 agccgaccct gccgttctcg tctctgacct cgacgagctc gtctttgtag aaccgtcgtc
      421 ccagtttggt gacccgcgca cctcttagct tctcgcgggg cagattaggc gcgttgacgt
      481 tcaggattct tccggagggg aggccgttga gagcgacctt caccaggctg gccgtgaact
      541 ccgccgcggc ctcgaagtgc agccctcgct cgagcggcat agcctcctgg acggtctcgt
      601 gccagggtcg gtttatgctc aaggagacgg cgatgccagg gaccccgatc actatcccct
      661 ccatcgcacc ggctaccgtc cccgaatacg tgatgtcgtc gccgaggttc tccccgtggt
      721 ttatccccga gacgacgatg tcaggctcga agtccatgag gccgagggcg acgagccgca
      781 cgcagtcgac cggcgtcccc gagcaggcgt agccgaccgt cccgtcggcc atcttccgct
      841 cctcgacgtg gagcgaacgc ccgaaagaga tgctcctccc cacgcctgag cggttgcggt
      901 cgggggccac ggtgatcacg tcccctacct tctcgagcgc gcgtcttgcg gcaagaagcc
      961 cgggagcctc tattccgtcg tcgttcgtca atacaatgcg catagtccca agctctccgg
     1021 ttctgtcgga ggtgatcgcc gtcccaaaag ggtcctgatt ctatcatccc ggccctgatt
     1081 gccatcgtgc ccatcaggta tagaaatggg ctatctgtga cattaagaaa aaagaatgga
     1141 caaagccgct cacatacggt agcttaccgt ggctgtgacc gagagggcta cgtaaggagt
     1201 gtgatgcagg tggacgccgt tgagtggttt aagcatcgct cctatgacta caagcagttc
     1261 tctgacctca gggcactggg ccgccgcaag cgcgagctcg gcctgacggt cagcgcggtc
     1321 ctgccctgcc gcaacgtcgc cgacaccgtg ggcgggatca tcgatgagat caacgccgtc
     1381 aaccgtcaga cgggggatac tcctctggtc gaccagatcc tggccgtaga cgccgactcg
     1441 cccgacggca ccgccgaggt ggcggcatcg aggggcgccg aggtgtactc ggagaacgag
     1501 ctgatgagca accacggcgg cgcccacggc aagggcgacg ccatgtggcg ctctctctcc
     1561 gtggcgcgcg gcgacctcgt catgtacatc gacgcggaca ccagggactt caggccccag
     1621 ctcgtatacg gcatcctcgg cccgatcctg gaggtacccg agatccgctt cgtcaaggcc
     1681 gcctatagac ggcccttcaa gagccacgag accgtggagg ccgacggtgg aggaagggtt
     1741 acagagctct cgaccaagcc gctcttcaac ctcttctacc cggaattgac cggattcgtg
     1801 cagcctctgg ccggagagtt cgtcgcagac agggagctct tctcctccat ccccttcctc
     1861 actggctacg ctgtggagac ggggatcatg atagacgtac tcaagatggt cgggctcgat
     1921 gcgatggccc aggtggacct cggtacgcgc cagaacaggc accagccact gcgcgacctc
     1981 tcccgcatgt cctactcggt cctgcgcgct gtcgccaggc ggatgcgcca ggagggacgc
     2041 ctgaaccagg tgcgcgaccc cggtctgccc gactccctct tccagttctc cgactacatg
     2101 cacgccgtcg ccacgccgga cggcctcaaa ctgcaggagt acgtcgagga gctcgtcgag
     2161 cggcccccga tcaaggaagt attgagggtc ggataggagc cggatcctgg gcggagtcca
     2221 cgacctcgga ggttcgccgg gcggtcccat agacaggggc cagcacgaag tcgaggactg
     2281 ggaacgcctc gccgacgcca tcaccacggc gctcgacggc aaggggatca agaccacaga
     2341 cgaacaccgc agggcgatag agagcctcga aaactactac gagctctctt actacgaacg
     2401 ctgggcagcc gggaccgagc agctgctcgt cgagaagggc atcctcaccc gcgaggagat
     2461 agacgagagg gcggcccgga tcggccgccg ctggaacgga ccgtgacctt caagccgggc
     2521 gaccgcgtaa gggtcgtgcc cgcagagaag ccgggccacg tacgcacccc ggggtacatc
     2581 aaagggaaga ggggctggat cgagtccgtg ctcggcgagt tcccgaaccc cgaagacctc
     2641 gcatacggct tctccggcct gcccgaaaga ctactctaca aagtgggctt ccggcaggcc
     2701 gacctctgga gagactacga tggacccgcc gatgattgcc tgtacgccga cgtctatgaa
     2761 cactggctcg agcccggcga aggaggaacc gcgtgagcga ccatcacccg cacgaggaga
     2821 cagcgaccag cccgtacgcc gcgcgcatcc gtgccataga ggcgctgctc gtggagaaag
     2881 gagtcctcac ggaaccggag gtgcaggaca ggatctccta catggaggca cgctccccag
     2941 ccaacggcgc gaagctcgtg gcccgcgcat gggttaacga ggacttcaaa gagttgctcc
     3001 tctccgacgg caagtccgct gcctctgagc ttggcataga ccccgaacac cccgctgagt
     3061 tcgtcgtcgt agagaacacc cctgaggtac acaacttgat cgtctgcacc ctctgctcct
     3121 gctacccgcg ggccatcctc ggccgtccgc cagactggta caagagcttc aactaccgct
     3181 cgcgcgccgt cagcgaaccc cgcgcggtca tgcgagagtt tgggttcgag atccccgaag
     3241 gcgtcgaggt agccgtccac gacagctcgg cggacattcg ctatctcgtc ctgccgatgc
     3301 gtcctcccag cacggaaggc atgagtgagg aggagttggc cggcctcgtc acgcgggact
     3361 gcctgatcgg ggtctccgtg cctctagagc cggtcttaac cggctagcaa tcgtggtgat
     3421 cccgttgcac acgcttgcga cgccgagcgg atcctcaaac tccagtatcc tttgctactg
     3481 ttcctctgtg tggagccggt ccggccttct acgattcatg ccactctctc tacccgtaca
     3541 acggcgatca cgttccgccg agaacgggaa cct
//
LOCUS       DATAIR010000582         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_156830, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000582 DATAIR010000000
VERSION     DATAIR010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156830"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VHM69_13855"
     CDS             <1..832
                     /locus_tag="VHM69_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX2741525.1"
                     /translation="NLHADLRRLGFSRKMLQGYEAVFRRPYGTILVTGPTGSGKSTTL
                     YATLGEINAPEKNIITVEDPVEYRMRGINQVQVNPRIGLTFPSGLRSILRSDPDVVMI
                     GEIRDRETARTSIEAALTGHLVLATLHTNDAPAAVTRLNDMGVEPFLTASAVDCVIAQ
                     RLARRLCDRCKRPVEMDERALRDLGFPTTRLANVKRDFHEAVGCERCGGTGYRGRIGI
                     YEMMIVDQEIRRLILRSASADAIARAAKKEGTIPLREDGLIKAALGITSVEEILLTIV
                     "
     gene            1364..2773
                     /locus_tag="VHM69_13860"
     CDS             1364..2773
                     /locus_tag="VHM69_13860"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX2741526.1"
                     /translation="MRTFRSRPDLRPPAVTVNMNSPDTTPGYVFVSPKKGPGEEAPSE
                     DAPLIVDNVGEPVWFHPLPSADEDAFNFEVQTYKGEPVLTWWEGLHTGYGQGEYVIYD
                     QSYSEIRRFKAGNGYEGDHHEFLITPEDTALITIYSKVQMDLSSVGGPADGVVLDGIV
                     QEIDIETGEVIFEWHSLDHITVDESLYNPPPDLESSFDYFHINSIDPFPDGYLLISAR
                     RTSAVYKVDRKTGEVVWRLGGEKTDFEMGPGTRTDWQHDARYHPDGTITIFDNGGVSK
                     DVQSRCIVVEIDEDAMSATLVGEYTHPDEILAETQGNMQVLPNGNVFVGWGSEPYFSE
                     FSRDGKLLFDASFPTEVESYRAFRFPWKGQPKVPPALVAESGTDGNVTLYVSWNGATE
                     VATWHVLAGSGPEELEHVGSAPRKGFETTITLQTSDPYVAVRAEDHTGKLLGTSRTIQ
                     LQNSEASSSATRSRPKRTA"
BASE COUNT          610 a          890 c          915 g          535 t
ORIGIN      
        1 gaacctccac gccgaccttc gacgtctcgg gttctcacgc aagatgcttc aagggtacga
       61 ggccgtattc aggagaccct acgggacgat tctcgtaaca ggacctacgg gcagcggtaa
      121 gtcaacgacc ctctacgcca cactggggga gatcaatgct cccgaaaaga acatcatcac
      181 cgttgaggac cccgtcgagt acaggatgcg cggtatcaat caggtccagg tcaaccccag
      241 gatcgggctg accttccctt ctggtctcag gagcatcctg cgctctgacc ctgacgtggt
      301 gatgatcggg gagataaggg accgtgagac cgccaggacc agcatcgagg ccgcgctcac
      361 gggccacctc gtactggcca cgctgcacac caacgacgcc cctgctgcgg tcacgcgctt
      421 gaacgatatg ggagtggagc ctttcctgac ggcatcagcg gttgactgcg tcatcgccca
      481 gaggctggcg cggaggctct gcgatcggtg caagcggcct gtcgagatgg atgaacgggc
      541 cctgcgagat ctcggcttcc caaccacgcg tctggcgaac gtaaagaggg atttccacga
      601 ggccgtgggt tgcgagcggt gtggtggcac cggctacagg ggacgcatag ggatctacga
      661 gatgatgata gtggaccagg agataaggcg gttgatcttg cgtagcgcat cagccgacgc
      721 gatagctcgc gcagcaaaga aggaaggcac gatcccactc agagaagacg gcctcataaa
      781 agctgccctg ggtattacca gcgtcgagga gatcttgctg acgatcgtgt gacaggctga
      841 ccctacgcgc aaggcccgta gcgatcggtc ggctcatcgc tccagtttga ccgagaatcc
      901 cgactttcta aggcaggcta tagagtttgc ccgccggtga gagttacggg ttagtccgca
      961 tactcgacgc aatttgacat gggccatggg tgtctgatct tgcaaatccc ggctttttca
     1021 tggaagatct ccgtcgtcgg gacgtccgcg aagagagtgc gcgcactcat gaagcgctcg
     1081 tgccgcaact gcgaccggat atagtgtacg gcgggtagcc ttcgtggctt ccgctcgtgg
     1141 agagggcggc tttgccctgt ttggtggagc aacgctagcg agagacgaga ggtcgaattt
     1201 gggtgagcag gcaggtaaca ggagtctaag agcggcgatc acccgtcgaa gatttctcgg
     1261 cgcacttacg gccggaacag cctggatcgc cctggccggg acccttggct gcgagccgga
     1321 cgcgcggact cgggccaccg cagccccaaa gggacaggga cccgtgagga ctttccgatc
     1381 acgcccggat ctccgccctc ccgccgtaac ggtcaacatg aactctccgg atacgactcc
     1441 cggttacgtc ttcgtctccc cgaagaaagg tcccggcgaa gaagctccca gcgaggacgc
     1501 gccgctcatc gtcgataacg tcggcgagcc ggtctggttc catccgctgc ccagtgccga
     1561 cgaggatgct ttcaacttcg aggtgcagac atacaagggt gagcccgtgc tcacctggtg
     1621 ggagggtcta cacaccggct acggccaggg tgagtacgtc atctacgacc agtcgtacag
     1681 tgagataaga aggttcaaag ccggcaacgg ctacgaggga gaccaccacg aattcctcat
     1741 cacgcctgag gacaccgcat tgatcacgat ctatagcaag gtgcagatgg acctctcttc
     1801 ggtcggcggt cccgctgacg gcgtggtgct cgacgggatc gtccaggaga tcgacatcga
     1861 gaccggcgag gtgatcttcg agtggcacag cctcgatcac attaccgtcg atgagtcact
     1921 ttacaatccg ccgcctgacc tcgagtcgtc cttcgattac ttccacatca actcgataga
     1981 tccgtttccg gacggatacc tgctgatctc ggcgcgcagg acttcggccg tgtacaaggt
     2041 cgaccgaaag accggagagg tcgtctggcg gctgggcgga gagaagaccg acttcgagat
     2101 gggtccgggg acccgcacgg actggcagca cgacgcaaga taccaccctg acggcaccat
     2161 cacgatcttc gacaacggcg gggtgtccaa ggacgtgcaa tcgcgctgca tcgtcgtcga
     2221 gatcgacgag gacgccatga gcgccacgct cgtcggtgaa tacacgcatc cggacgagat
     2281 actcgccgaa acccagggaa acatgcaggt tttgcctaac ggcaacgttt tcgtagggtg
     2341 gggtagtgag ccctacttct ccgagttcag ccgcgacggc aagctgctct tcgacgccag
     2401 cttcccgacc gaggtcgagt cctatcgggc tttccgcttt ccgtggaagg gccagccgaa
     2461 agtcccgccc gcactagtcg ccgaatccgg gaccgatggc aacgtaacgc tctacgtcag
     2521 ctggaacggt gccacggagg tcgccacctg gcacgtgctc gctggttccg gccccgaaga
     2581 actcgagcac gtcggctcag caccccggaa gggcttcgag accaccatca cgctgcaaac
     2641 cagcgatccg tacgtcgccg tgcgcgccga ggaccataca ggcaagttac tcggcacttc
     2701 caggacgatt caactccaga acagcgaagc ctcctcttct gcgacgaggt cacgcccgaa
     2761 acggactgca tgagcgtttc cgcgcgcccg gaaggcaagc tgtcgcgtcc gcgctcgtgg
     2821 agcttgatct gcggagtacc gggatagtga aggggcgggc tccgggccca tggttcgagg
     2881 ccggcgtggg actctccgcg ggtgcttcct tcgcggtagt cggtccattg cgagacgttt
     2941 tctcgacgct
//
LOCUS       DATAIR010000583         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_409225, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000583 DATAIR010000000
VERSION     DATAIR010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409225"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1811)
                     /locus_tag="VHM69_13865"
     CDS             complement(33..1811)
                     /locus_tag="VHM69_13865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX2741527.1"
                     /translation="MAYEFARYLKIRGAWGASFSPDGRRVSFLTEITGVPQVWEVAAD
                     GPSWPEQLTFYEERVSDATYSPTENSLLFSMDAGGNERSQLFLLQDGEVMDLTGAPDA
                     IHYSGDFSSDGRRIAYTATRRNGTDFDVFVQNLPDGEPEMVWKVSGYHTVADWAPEGS
                     ALIVSRHHSNVNNDLYWLDLATGEATLLTPHEGDARFYGANVTPEGGSIYLATDRDGE
                     FLRLARLELASLTLEYLTPDDWDVESVELSRNGRYIVASRNVDGYSDLMLFNGRGRRM
                     PDPRIPEGIVGGLEFSPDSGRLAFTLTASERNPDVWVLDLPDGDARRLTRSSTAGIPP
                     RTFRRPRVVRYPSFDGRRIPALFHEPGIENAPVIVNVHGGPESQSRPAFAPVTQYLLH
                     RGYAVFFPNVRGSTGYGKTYTHLDDVELRMDSVKDLAHAAYWLREQGHERIAVMGGSY
                     GGFMVLAALTEYPGLWTAGVDIVGIANLVTFLENTGSYRRHLREPEYGSLEKDRAFLS
                     SISPIHKAEKIKVPLMVVHGKNDPRVPVGEAEQIVERVKNNGGIVEYLLYEDEGHGLA
                     RLKNRLDAYPRIAAFLEEHLAGRRAD"
     gene            1930..2367
                     /locus_tag="VHM69_13870"
     CDS             1930..2367
                     /locus_tag="VHM69_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741528.1"
                     /translation="MLRRLRNIFGGAAPSTQEPSPLYGLAEEERERIGTSEDPLRNFE
                     AAMQRNEEAERAVHNGDTERAIQLYETSIAEEFVGSHPYERLASIYERRHNPAEALRV
                     CIAFTTLAASGKMPRGAQRSADRKLPEFEARIERYSRLIDDSQ"
     gene            2575..2934
                     /locus_tag="VHM69_13875"
     CDS             2575..2934
                     /locus_tag="VHM69_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX2741529.1"
                     /translation="MKSYLKMLLVAGAFVLSLAAFQDDAQADTMLASWYGPGLEGNVT
                     ASGDIFEPYTEYTAASPYLPFGTDLLVTYGDYSVVVEVTDRGPYVGGRDLDLSAVAAE
                     EIGLTYVGTDYVDVEVI"
     gene            3214..3651
                     /locus_tag="VHM69_13880"
     CDS             3214..3651
                     /locus_tag="VHM69_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2741530.1"
                     /translation="MSAVDDLDQAVEQYQEALGEFMKGNPEPAKKVWSRREDVTLANP
                     LVGSPARGWEQVSETMEHAASQVRDGEITGFETVAKYVTPDLAYVVWVERARAKVAGR
                     EDVVPLDLRVTMIFRPEDGAWKIVHRHADPITTAQPAESVIQQ"
BASE COUNT          869 a         1304 c         1411 g          844 t
ORIGIN      
        1 gaggtgcgcg ggtagcgacc cgcgaagccc tatcaatcgg cccggcggcc tgcgagatgc
       61 tcctcgagga aggctgctat cctggggtag gcgtcgagac ggttcttcag cctggcgagg
      121 ccgtgtcctt cgtcttcgta gagaaggtac tcgacaattc cgccgttgtt cttcacacgc
      181 tccacgatct gctctgcctc cccgacaggc accctggggt cgttcttgcc gtggacgacc
      241 atgaggggca ccttgatctt ctcggccttg tgtatgggac tgatcgacga gaggaaggcg
      301 cggtccttct ccaacgaacc atactcgggc tcgcgcaggt gcctccggta gctgcccgtg
      361 ttctccagaa acgtaaccag gttggcgata cccacgatgt ccacccctgc cgtccagagt
      421 cccggatact cggtgagggc ggccagcacc atgaacccgc cgtaggagcc gcccatgacc
      481 gcgatacgct cgtggccttg ctccctgagc cagtacgccg cgtgcgccag atccttgacg
      541 gagtccatcc gcagctcgac gtcgtcgagg tgggtgtagg tcttgccgta gccagtcgag
      601 ccgcggacgt tcgggaagaa cacggcgtag ccccgatgca gcaggtactg cgtaacaggg
      661 gcgaacgcgg ggcgcgactg gctctccggc ccgccgtgca cgttgacgat cacaggcgcg
      721 ttctctatcc ccggctcgtg gaagagggcc ggtatccggc gcccgtcgaa gctgggatag
      781 cgtacgaccc gcggtctgcg gaaggtgcgt ggcgggatac ccgccgtcga ggagcgggtg
      841 aggcgcctcg cgtcgccgtc cggcaggtcg aggacccaga cgtccgggtt acgctcagag
      901 gccgtgaggg taaaggcgag ccgaccggag tcaggagaga actccaatcc gccgacgatg
      961 ccttccggta tgcgcgggtc cggcatcctg cgtcccctgc cgttgaagag catcaggtct
     1021 gaatatccat ccacgttgcg gctcgcgacg atgtagcgac cgttcctcga aagctcgacg
     1081 ctctcgacgt cccaatcgtc cggggtgaga tattcgagcg tgagcgacgc gagctccagg
     1141 cgggcgaggc gcaggaactc cccgtcgcgg tccgtcgcca ggtagatgct gccgccttcg
     1201 ggcgtgacgt tcgcgccgta gaaccgcgcg tccccctcgt gcggggtaag tagagtcgcc
     1261 tcgcctgttg cgaggtccaa ccagtacagg tcgttgttca cgttggagtg gtggcgggag
     1321 acgatcaggg cactcccttc aggtgcccag tccgcaacgg tatggtatcc ggagaccttc
     1381 cagaccatct caggctcccc atccggaagg ttctggacga atacgtcgaa gtccgtcccg
     1441 ttgcggcggg tcgccgtata cgctatgcgc cgcccgtcag acgagaagtc tccagagtag
     1501 tggatggcgt cgggagcacc cgtaaggtcc ataacctcgc cgtcctggag caggaagagt
     1561 tgcgagcgtt cgttgccgcc ggcgtccatg ctgaagagga gagagttctc ggtcggcgaa
     1621 tacgttgcgt ccgagacgcg ctcctcgtag aaggtcaact gctccggcca ggaaggacca
     1681 tctgccgcaa cctcccacac ctgcggtacg ccggtgatct ccgtcaggaa cgagaccctg
     1741 cgcccgtcgg gcgagaagct cgcgccccag gcgccccgga tcttgagata ccgcgcgaac
     1801 tcgtaggcca atgttctccc gtttctccga tacggctgag cggagattaa aacacaatgg
     1861 agatcccggc ttcgcccgag actcaccgct tggttatact caggcgagcg tatcgggagg
     1921 gaaggcttgg tgctaaggcg tctgcggaac atcttcggag gggccgcccc gtccacacag
     1981 gaaccatcgc ctctctatgg actcgccgaa gaagaacggg agaggatcgg cacgtcggag
     2041 gacccgctcc gcaacttcga ggcggcgatg cagcgcaacg aggaggccga gcgtgccgtt
     2101 cacaacggcg acaccgagcg ggccatccag ctctacgaga cgagcatcgc agaggagttc
     2161 gtcggcagcc acccttacga gaggctcgcc tccatctacg aacgcaggca taacccggcc
     2221 gaagccctcc gcgtctgcat tgccttcacg acgctcgccg ccagcggaaa gatgccccgc
     2281 ggagcccagc gctcggccga ccgcaaactc cccgaattcg aggccaggat cgaacgctac
     2341 agtcgcttaa tcgacgatag ccagtaaatc cagcataacc gcctgaccgc cgcttgccgg
     2401 cagaaaaccc ccatatgtag gtaaaaagcg tcgctgacgc catatctcgt ggatcagatg
     2461 tggacgtaac gcgttcacaa acaggtctca taaacgtcat gtagcgactt gtaacttgat
     2521 tgtagcgtag taaggtcttg ggcttgttgt gcaagcgatc tggaggggga cttcttgaag
     2581 tcttatctca agatgctctt ggtggctggt gctttcgtcc tatcgctggc tgcctttcag
     2641 gatgatgccc aggccgacac catgctggcg agctggtatg gaccggggtt ggagggcaac
     2701 gtgacggcga gtggggacat cttcgagccc tacaccgagt acactgcggc gtcgccgtac
     2761 ctgccattcg gtacggactt gctggtgact tacggggact actccgtggt cgtcgaggtc
     2821 acggaccgtg ggccgtacgt gggaggcagg gacctcgacc tctccgcggt ggccgccgag
     2881 gagatcgggc tgacctacgt cggcaccgac tacgtggacg ttgaggtcat ctagtagggt
     2941 cctcgccgga acatcggtga gaagcaagag cgggcgcggc attgatgccg cgcccgtttt
     3001 ctttgcaaca gtcgccacat cttcaggaat cagaggcctg tctggccgga aatagtgtac
     3061 gagggacgct gctggcgata gaaggtttct ctttgccttg cgggcactgc ggtgaatagg
     3121 gcaagatgtg tcagagcctc ggccacgggg aacgagtatg tcgcaccttg cgggaaggtt
     3181 tacttaccga cgaaggagga gatctcctag aagatgtccg cggtagatga tcttgaccag
     3241 gcagttgagc aataccaaga agcgctgggc gagttcatga aagggaaccc cgagcctgcg
     3301 aagaaggtat ggtcccgtcg agaagatgta accctcgcta accccttagt aggctctcct
     3361 gcgcgtggat gggagcaggt ttcggagacc atggagcatg ccgcatcgca ggtcagagac
     3421 ggcgagatca ccggcttcga gacggtggcg aagtacgtga ctccggatct tgcctacgta
     3481 gtgtgggtag agcgggccag ggccaaggtt gccggaaggg aggatgtcgt tccattggat
     3541 ctgcgggtta cgatgatctt tcgacccgag gacggcgcgt ggaagatagt gcatcgacac
     3601 gcggacccga taaccacggc tcagccggca gagtcggtta ttcagcaata gctctacctt
     3661 aggcgaactt ccacaaccag attccgagaa agtggtattt ctccgaagtt gggcgatgtc
     3721 ttcgccctta ggggccgcta tgcttggacc ggatttcgcg ttcaattccg taccgactaa
     3781 gagaggagct atgggcaagc gatgataagg gcttcgtgtc attgcggttc cgtccacctg
     3841 gagatcgacg ttccgccgtc ggaggttacg gagtgtaact gttcgatatg ccgacgctac
     3901 ggcgttttgt gggcttacta ccctctggat cgagtgcgcg tgttgccctc cgatccgctg
     3961 accgacgtct acgtatggaa cggtaggacc accgaattcc accgctgtcg gaattgcggc
     4021 tgtgtctcgc attggtcccc ggtgaatcgc gagcgcaacc gcatgggcgt caatgcgcgc
     4081 ctcatggaac cggaaatcgt cgccggcgcc cgcgtacgcc accttgatgg tgccaacacc
     4141 gaggagtata tcgactgact atcggcggtt aggccaacaa tcgagtatcg cgctcccgaa
     4201 gggcgaccaa gcgatctagt tgcgccttat tcacccaacc ttgcagaagg ataattctga
     4261 gaagctcgag gagcagctga ggcgaagatc actcaattgg ggcatgcgcc ggcttcactt
     4321 ccggtctacg ataccttcgt tacgtatgcg aagcggaagg gagcatcccg tggcggcgaa
     4381 agaggacagg aagatcttca tcggtccgga cgagggcgtc caccttcc
//
LOCUS       DATAIR010000584         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_39512, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000584 DATAIR010000000
VERSION     DATAIR010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39512"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1180)
                     /locus_tag="VHM69_13885"
     CDS             complement(302..1180)
                     /locus_tag="VHM69_13885"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2741531.1"
                     /translation="MTMPSFLIIGAMKSGTTALYYYLAQHPQVYMSPVKEPNYFCSER
                     QASRDDYIAHAGTYQGLFEGASDEKAIGEASHCYLYEPEAVARIQQYLPEARFIAILR
                     YPVDRAYSHFLHMVRTGDEPLTDFAQALREEQVRADPNRRFQDYIGRGLYYDQVKRYL
                     DVFGRKRVKVYLYEDLNNAPVDMLQDTFRFLEVDSSFIPDVSLRRNVSGHPRYKTLDK
                     VLRRPSPIKDALKMHLPAKLRWRLSKAFDDLKTRNLIVPPAVEPEVRQQLKDIYREDI
                     MKLQELIDRDLSKWLE"
     gene            complement(1274..1423)
                     /locus_tag="VHM69_13890"
     CDS             complement(1274..1423)
                     /locus_tag="VHM69_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741532.1"
                     /translation="MLPINSVGGAGVTVSPFGRNATAAFETLGWLLLAAAGALQRKLV
                     VYPEG"
     gene            complement(1455..2591)
                     /locus_tag="VHM69_13895"
     CDS             complement(1455..2591)
                     /locus_tag="VHM69_13895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2741533.1"
                     /translation="MLHVVGDFSSGGAERVAANLLRTLDPEQFDARAISLRGPLGSAL
                     EQTLAEDKIPVWYLGKEKGFNPLVLARVTRTMERFRPQVVHTHIYALRYAFPYMLFRR
                     VPAMVHTVHNLAEREIEWYGRWVHRLAFRRGVLPVAIAGEVAESIRRVYGIEDFPLIP
                     NGIPVNRFRDPSIGREGWRNKEGFAPTDVLFVCVATLRAQKNPALLVESFCRGAASDP
                     RAHLLFAGVADLKSGDLVSKLERQIAALGLQERVHLLGLRSDIPELLNAADVFVLSSD
                     YEGNPLAVLEAMAAGKPIISTAVGGVPELVEGGCGLLVPPGDAQALSEAMRHMLESPE
                     ARKSMGEASARRAVEHFDLRVMTGAYEDLYRTLIANDRSRWKNA"
     gene            complement(2928..>3195)
                     /locus_tag="VHM69_13900"
     CDS             complement(2928..>3195)
                     /locus_tag="VHM69_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741534.1"
                     /translation="SELGLVGLVLFLGILFFAFKTAVPIAQGSGLGTGMVLGLIVFII
                     AGMTLTWETQKIGYVLFGSVLALQLHNSIRRARSSAKQKDSPAD"
BASE COUNT          683 a          989 c          866 g          657 t
ORIGIN      
        1 cgacgctaca ggcctgcgcc gggttcactt ccactgcaat ccgccgtaga actaatatct
       61 cattgcgggt tgcgccgata tcgacccaca cacgcgaatc atgcacaacc ggtcgaggag
      121 acgcttcggc tgactcctct gtctgcgcac gtcatgcgat acgtgtcaac tacgctgaaa
      181 tccggtcaga tcgacgctgc ggaggccatc cgcagcgtcg atccgcaccg gcaaagtggc
      241 gcgtcatcac agcggcagac tactcgaaca atgaccgtag atcctggcat agaccaggag
      301 ctcattcaag ccatttcgag aggtcgcgat ctatgagttc ctggagcttc atgatgtctt
      361 cccggtagat gtccttcagt tgctgtcgca cctccggctc gaccgccgga ggtacgatca
      421 ggtttcgagt cttgagatcg tcgaaagcct tggagagacg ccaccgcaac ttggccggca
      481 ggtgcatctt gagggcgtct ttgatcggac tgggccttct cagtaccttg tccagggttt
      541 tgtatctagg atggccggac acattacgtc gaagggatac atccgggatg aatgagctgt
      601 cgacctccag aaaccggaag gtgtcctgca gcatatccac aggcgcgtta ttcaggtcct
      661 cgtagagata gactttgact cgcttccgac cgaacacatc cagatacctc ttcacttgat
      721 catagtagag tcctctgccg atgtagtcct gaaaacgcct atttggatct gcacggacct
      781 gttcttcccg gagtgcttgg gcaaagtcgg taaggggttc atcgcctgtc cgaaccatat
      841 gtagaaagtg cgagtacgct ctgtcgaccg ggtaacgaag aatagcgatg aacctggcct
      901 caggcagata ttgctgaatc cgtgcaacag cttccggttc atagaggtag caatgggacg
      961 cttcaccgat agccttctca tccgacgcac cctcaaagag cccctggtag gttccggcgt
     1021 gggcaatgta gtcgtcccga ctcgcctgcc gctcagagca gaaatagttg ggttccttca
     1081 ccgggctcat gtaaacctga gggtgttgcg cgagatagta atacagtgcc gttgttccag
     1141 atttcatcgc ccctataatg agaaaactcg gcatcgtcat agattgccca tccttgtttt
     1201 ggcgtagagc ggggagctac ggcccagcct cgcacatgaa ggtgatccgt gttcaaaaag
     1261 cacgtgacgc ccgctagccc tctggataca ctaccagctt gcgctgaagg gcaccggctg
     1321 cggccaaaag cagccagccc aaggtctcga acgccgccgt agcgttcctc ccgaacgggc
     1381 tcacggtcac tcccgcgccc ccgacgctgt ttatcggcag cacagccctc cttgcaagac
     1441 gaccggatat agggctaagc atttttccat cgagaccgat cgtttgcgat gagcgttctg
     1501 tacaagtctt cgtaagcccc cgtcatcacc ctcaggtcaa aatgctctac ggctcgtctc
     1561 gccgacgcct ctcccatgga cttcctggcc tcggggcttt ccagcatatg cctcatggcc
     1621 tcagacaaag cctgtgcgtc gccgggcggc acgagtaacc cgcacccgcc ctcgaccaac
     1681 tccggaaccc cgcccacggc ggtagagatg attggtttgc ccgcggccat agcctccaaa
     1741 accgcaagcg ggttgccttc gtaatcggag ctcagcacga agacatcagc cgcgttcagc
     1801 agctctggaa tgtccgagcg tagcccgagc aggtggaccc tttcttgcag gcccaaagca
     1861 gcgatctgtc tctcgagctt cgacaccaag tctcccgact tcaagtctgc tactcctgca
     1921 aaaaggaggt gggcccgagg gtcagatgcc gctcccctgc aaaacgactc cacgagcaag
     1981 gcaggattct tctgcgccct caacgtggct acacaaacaa agagaacgtc cgtaggcgca
     2041 aacccttcct tgtttcgcca cccttcgcgc ccgatcgacg gatcgcgaaa cctatttacc
     2101 gggataccgt tcgggatcag ggggaagtcc tcgatcccgt atactcgacg gatactctca
     2161 gccacctcgc ctgcgattgc cacgggaagc acacctcgcc tgaaagccag ccggtgcacc
     2221 cagcgcccgt accactctat ctccctttcg gcgaggttat ggacggtgtg taccatcgca
     2281 ggcactcggc ggaacagcat atacggaaag gcgtagcgca gagcataaat gtgagtatgt
     2341 actacctggg ggcgaaagcg ttccatggtg cgggtaaccc tcgcaagcac aagtgggttg
     2401 aagcctttct ccttgcccaa gtaccacacc ggtattttgt cctccgcgag agtctgctcc
     2461 agggcagagc ctaacggacc gcgcaagctt atcgcccgcg catcgaactg ctctgggtcc
     2521 agggttcgca gcaggttggc ggctacccgc tcggctccgc cgctgctgaa atcgccaaca
     2581 acatgcagca cccttatcaa cggagcccct cgagcttggc tggcgaaggg gcgcgttgcg
     2641 ttaaccctcg caggcggcgc tcaagagcgg agccgccgtg gggcccgccc ctatccagaa
     2701 cgcggatgag cagcacagac gtttgcgggt taaaggcccg gcacttactt tgttgcccca
     2761 cagaggtttc ctcttgctct agggtctctt ccacatcctt accgaatgga tggtcaaata
     2821 aaccgagtgg ccgggcttcg acccgttctt ggctcaggat agacaccgag ttagccgcga
     2881 ggcactcggc tgcgccgctg cgtaggccgg gcagtaggta caacgcctta atcagcggga
     2941 gagtccttct gtttggccga tgaccgtgcc cgccggatcg aattgtgcag ttggagcgcg
     3001 aggaccgaac cgaagagaac gtaccctatt ttctgggtct cccacgttag cgtcataccc
     3061 gcgattataa acacgataag accgaggacc atgcctgttc ccaggcccga cccttgggcg
     3121 atcggcaccg ccgttttaaa cgcgaagaac agtattccca ggaatagtac cagccccacg
     3181 agccctaact cgctc
//
LOCUS       DATAIR010000585         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_156942, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000585 DATAIR010000000
VERSION     DATAIR010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156942"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHM69_13905"
     CDS             <1..196
                     /locus_tag="VHM69_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741535.1"
                     /translation="RTSSAGVSTAFLSAMEDFVAVSGGVVGLFLPFLPLLFVGFLLFF
                     FNRIRKRRGRKYGGLRILGD"
     gene            224..1207
                     /locus_tag="VHM69_13910"
     CDS             224..1207
                     /locus_tag="VHM69_13910"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HEX2741536.1"
                     /translation="MDEREITIVKGGRETPFSRGILAQTLAQAGARAELAHQIASEVR
                     AELLAEQRYTVEEEEVLARVRDKLVKKDAFVVGRLDKWRLLRESTEPIVVLIGGATGV
                     GTSTLAADVARRLNIQSVVGTDSIREVLRRAISPDLLPLLHRSSYDVKREDLRVPVEE
                     EEMVLYGFRQQASQVAVGVEAIVDRGLKEGTNLVVEGVHLVPEIILHRYRDHPNVCTL
                     VVYLSDEHVHRSRFYIRALGTAMRRPAEEYISYFQEIRQIHDYILESSARAGVQAVEN
                     ISIENTSDAAVEIVANRVSGIAERAGQRASAMLLDGGTPRHGVPITRAKSG"
     gene            1238..1657
                     /locus_tag="VHM69_13915"
     CDS             1238..1657
                     /locus_tag="VHM69_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX2741537.1"
                     /translation="MLNKQRLRTFVLGGAVGALVGILLAPRSGRELRGSLANRAGEAR
                     ERGRETYFEAQEQMRERLAEVREGSRQRHDTVDPGGPAGETTGVERPYLRDVSRAADG
                     EATGPDAAERSEELRRKVRETRERLRGRKDSDLGPER"
     gene            1654..2268
                     /locus_tag="VHM69_13920"
     CDS             1654..2268
                     /locus_tag="VHM69_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741538.1"
                     /translation="MRPTSFVPMIGEISAVDAGDVPGLEAVISYWTGDKYSAKDWFRE
                     LNSCWGTAGFVVRRGEEVQGFVVYAPPERLSRAARYPLGPIDDEAVLLAYVGGDTRTR
                     RRLLVRMLRDLRQRGVGRVEAIASDRGVSNHVPTSFLLESGWRPVRRATYRMSHFTLA
                     HIDLKSAVEVGQLARALVGRVKLPGIKAPVPGALVRSTGEQSGR"
     gene            complement(2275..2772)
                     /locus_tag="VHM69_13925"
     CDS             complement(2275..2772)
                     /locus_tag="VHM69_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741539.1"
                     /translation="MREIVLWLSGAALAFFVTAGAFLSLANLAGAPSDKNLTPDPSPK
                     RSGPALELSLDEGRLAALRPLPGQSLDLVVKNGGGEQLSDVNVTLGVSSENTALPNSR
                     YYRRTVEKLLPDEAVTVHFEFDLSSPDQHVERAPAPEPTRKILEIRATTPQGVSAVRT
                     AILPH"
     gene            complement(2769..>3839)
                     /locus_tag="VHM69_13930"
     CDS             complement(2769..>3839)
                     /locus_tag="VHM69_13930"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX2741540.1"
                     /translation="RVDPGLGPGTRALSSGPGYKGSPSWSPGGGRIAFTVDGYVVDRP
                     AGPGDLRRWTTRDFVAEDTEWISDDTLTMLGAPSATEATPSSVYRARAGKNSLELEKV
                     ARGVMAMSIGSEGLILALGTGTHESALALAHGSGEVYRLYTDPIEGDVTSLSPSPDGD
                     AVVLALRPPGDLQTSELRVFDLLEGKAREITRLDGNREILGTPQWTKHGVYFVAGKKD
                     TSLDDDGAEPLYNLYRTSTESGAPELAPGVGEDFVAASIRVSPEGERVAVIGRLNPKS
                     PANLYVLDIHAQNLRAVTTNEDMEIKTGPDDLAWSPGGKSVAIVARGTPSTEPEVRAA
                     PASSLLKDFYNLYEIPVEGTTR"
BASE COUNT          636 a         1163 c         1360 g          680 t
ORIGIN      
        1 gagaacctcc tcggccggcg tctcgacggc gttcttgagc gctatggagg actttgttgc
       61 cgtttcaggc ggggtagtag ggcttttcct gcccttcctg ccgcttttgt ttgtcgggtt
      121 tctgctattc ttcttcaacc gcatcaggaa gcggcggggg aggaagtacg ggggcctccg
      181 catcctcggc gactaatatt cgtcggcgct ctaggagggt gcaatggacg agcgggagat
      241 cacgattgtc aaggggggca gggagacacc gttctcccgg ggaattctgg cgcagacgct
      301 cgcgcaggcg ggcgcgcgcg cggagcttgc gcaccagata gcgagcgagg tgcgcgccga
      361 gctcctggcc gagcagcgct acacggtcga ggaggaggag gtccttgccc gcgtcaggga
      421 caagctggtc aagaaggatg ccttcgtcgt cggcaggctc gataagtggc gcctcctgcg
      481 ggagtcaaca gagcccatag tggtcttgat cggcggggcg acgggggtcg ggacgagcac
      541 gctcgcggcc gatgtagcca ggcgtctgaa tatccagagc gtggtcggta cggactccat
      601 cagggaggtc ctgcggcgtg cgatcagccc cgacctcttg cccttgctcc acaggagctc
      661 ctacgacgtg aagcgcgagg atctcagggt gccggtcgag gaggaggaga tggtcctcta
      721 cggcttcagg cagcaggctt cccaggtcgc cgtcggcgtg gaggccatcg tggaccgggg
      781 actgaaagag gggacgaacc tcgtcgtcga gggtgtgcac ctggtacccg agatcatcct
      841 ccaccgctac cgcgaccacc ctaacgtctg cacgctggtc gtgtacctct cggacgagca
      901 cgtgcatcgc tcgcgcttct acatcagggc cctcggcacg gctatgcgcc gtcctgctga
      961 ggagtacatc tcctacttcc aggagattcg ccagatccac gattacatcc tcgagagctc
     1021 cgccagggcc ggcgtccagg cggtcgagaa catctccata gagaacacct ccgacgcggc
     1081 cgtggagatc gtggccaaca gggtctctgg gatagccgaa agggccggcc aacgcgcctc
     1141 ggcgatgctc ctcgacgggg gtacgccacg gcacggggtg cccatcaccc gcgcgaaaag
     1201 cggttagaat atcccgagaa gcccagaagg agataaagtg ctgaacaagc agcgactgag
     1261 aaccttcgtg ctcggcggcg cggtgggggc cctggtgggc atactcctgg cgccccgaag
     1321 cggtcgtgag cttcgaggct ccctcgccaa cagggcgggc gaggcccgcg agagaggccg
     1381 cgagacgtat ttcgaggcgc aggagcagat gagggagcgt ctggccgagg tccgcgaagg
     1441 gtcccgccag cgccacgaca cggtggatcc gggcggaccc gccggggaga cgacgggtgt
     1501 tgagcggccc tatctcaggg acgtttcacg cgcggcggac ggcgaggcca cggggccgga
     1561 cgccgccgag aggtcagagg aactccgcag gaaggtgagg gagacaaggg agcggctgcg
     1621 tggacggaag gacagcgacc tggggccgga gcgatgagac ccacgtcttt cgtgccgatg
     1681 ataggtgaga tctcggccgt ggatgcaggc gatgtgcccg gcctggaggc ggtgatctcc
     1741 tactggaccg gcgataagta ttccgcgaaa gattggttcc gcgagctgaa tagctgctgg
     1801 ggtacggcgg gtttcgtcgt gcggcgtggt gaggaggttc aggggttcgt agtctacgcg
     1861 ccgccggagc gtctgtcgcg cgccgcgcgt taccctctgg ggccgataga tgatgaggcc
     1921 gtgctactgg cctacgtagg gggcgatacg cgcacacgcc gccgcctgct cgtgaggatg
     1981 ctcagggatc tcaggcagcg gggcgtaggg agggtggagg cgatcgccag cgaccgtggg
     2041 gtgtccaacc acgtgcctac ctcttttctt ctggagagcg gctggcggcc cgtgcgccgc
     2101 gccacttaca ggatgagcca cttcacgctc gcgcacatcg acctgaagag cgccgtcgag
     2161 gtggggcagc tggcgcgggc tctggttggt cgggtcaagc tgcccgggat caaagcaccg
     2221 gtaccaggcg cgcttgtccg ttcgactggc gagcagagcg gccggtagag gttctcagtg
     2281 aggcaggatc gcggtacgga ccgccgagac gccctgtggt gttgtggccc ttatctccag
     2341 gatcttacgc gttggttcgg gggcgggcgc cctctcgacg tgctgatcgg gtgaagagag
     2401 gtcgaactcg aagtgaacgg tgaccgcctc atccgggagg agtttttcaa ccgtccgccg
     2461 gtagtagcgg gagttcggaa gcgccgtgtt ctcggaggag acacccaggg tcacgttcac
     2521 gtcggagagt tgctcgccgc ccccgttctt taccaccagg tcgaggctct gcccgggtag
     2581 cggcctgagc gctgccagcc ggccttcgtc caggctcagt tcgagcgctg gcccggaccg
     2641 tttgggtgac ggatcgggcg tcagattttt gtcggaaggg gcgccagcaa ggttcgccag
     2701 ggacaggaaa gctcccgccg tgacgaagaa ggcaagcgcc gcgcccgaga gccagagaac
     2761 gatctccctc accgcgtggt accttcgacc gggatctcat acaggttgta gaagtctttc
     2821 aggaggctac tggcgggggc cgcacggacc tcgggttccg tggatggggt tccacgggcg
     2881 acgatcgcga cgctctttcc gcctggggac cacgccaggt cgtcaggccc tgtctttatc
     2941 tccatgtctt cgttggtcgt cacggccctg aggttctgcg cgtggatgtc gagtacgtac
     3001 aggttggccg gcgacttggg gttgaggcgg ccgatgacgg ccactcgttc gccctcggga
     3061 gagacgcgga tgctcgcggc tacgaagtcc tctccaacgc ccggcgcgag ttccggcgcg
     3121 ccgctctccg tcgaagtgcg atagaggttg tagaggggct ccgcgccgtc gtcatctagt
     3181 gaagtatctt tcttcccggc cacgaaatag acgccatgct tcgtccactg gggcgtaccc
     3241 aggatctccc ggttgccgtc cagccgcgtt atctctcggg ctttcccctc cagtaggtcg
     3301 aagacgcgca gctcggaggt ctggaggtcc ccgggagggc gcaaagccag gacgaccgcg
     3361 tcgccgtccg gcgaagggga gagggacgtg acgtcgcctt cgatggggtc ggtatagagc
     3421 cggtagactt ccccgctgcc gtgcgccagg gcgagtgcgc tctcgtgagt accggtgccg
     3481 agggcgagga tcaaaccctc gctaccgatg ctcatcgcca tgacccccct ggcgactttc
     3541 tcgagttcca gcgaattctt cccggcacgc gcccggtata cggaactcgg tgtcgcttcg
     3601 gtcgctgagg gcgctccgag catcgtcagg gtgtcgtccg agatccactc tgtatcctcg
     3661 gcgacgaagt ccctggtggt ccagcgcctc aggtctcccg ggccggccgg cctgtcgacc
     3721 acgtagccgt cgacggtgaa cgcgatcctg ccgcccccgg ggctccagct cggcgagcct
     3781 ttgtacccgg gacccgagct cagggcccgc gtcccagggc cgagtccagg gtcgaccct
//
LOCUS       DATAIR010000586         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_202667, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000586 DATAIR010000000
VERSION     DATAIR010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202667"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..969
                     /locus_tag="VHM69_13935"
     CDS             130..969
                     /locus_tag="VHM69_13935"
                     /inference="COORDINATES: protein motif:HMM:NF044469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HEX2741541.1"
                     /translation="MLQQEAMIERTRRVCKEDERLSAAMMYGSFAQGEGDGFSDIEFI
                     LFFRDEDLEGVDQEEWVSRISPVELYFVNEHGNGTAIFENLVRGEFHFDRASDMERVV
                     GESMKDADWLPSLEDTLILDRTGELTPRLRTIVGPPPARDTPENVLFLCNCFVNWFIF
                     GSNLFARGELARSLDLLGIVRDRLLQMVRVLEGSTLHWFNSSKFLEREISETSYRRYV
                     SCTARLDTDELRRAYLSAWEWGRELMPSLADGYGVVLPADLLLRLDGRFAETFRTAEQ
                     EGP"
     gene            966..1523
                     /locus_tag="VHM69_13940"
     CDS             966..1523
                     /locus_tag="VHM69_13940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2741542.1"
                     /translation="MKETENPWRTLGSKRVYENPWISVREDRVIRPDGEPGIYGVVHY
                     KNRAVGVLPVEDGHVYLVGQYRYPLAHYSWEIPEGGCPEDEEPLRAAQRELKEETGLE
                     ARNWRMLGEAYLSNSVADEHAVWFLATGLVPGEQQPEGTEVIGVRRVPVSEALAMAMD
                     GRITDALSVLALTTYALDQSRGNDG"
     gene            1551..>1753
                     /locus_tag="VHM69_13945"
     CDS             1551..>1753
                     /locus_tag="VHM69_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX2741543.1"
                     /translation="MKVCALAGGVGGAKLAAGLQDVLPAGDLAVVVNTADDFDLWGLR
                     ICPDLDTVMYTLAGISNPETGWGL"
BASE COUNT          325 a          546 c          581 g          301 t
ORIGIN      
        1 ccagcgacga gtaccgctac gagcttggag agcgcggctg ggccgagacg cgtgcaggtt
       61 cctccggatt gcatcgaccg cgatgctaag atatcagccc ggaaataatg ccggatagta
      121 tggaggacga tgctccagca ggaagctatg atcgaacgca cgcgccgcgt ctgcaaggag
      181 gacgaacgcc tctccgcggc gatgatgtac ggctccttcg cccagggcga gggggacggg
      241 ttctcggata tcgagttcat cctgtttttc agggacgagg acctcgaggg cgtggaccag
      301 gaggagtggg tgtccaggat ctcacccgtc gaactgtact tcgtgaacga gcacgggaac
      361 gggacggcga tcttcgagaa cctcgtcagg ggcgagttcc atttcgaccg cgcctcggac
      421 atggagaggg tcgttggcga gtcaatgaag gacgcggact ggcttccctc gctcgaagac
      481 accctgatcc tggaccgcac gggcgagctg accccgcgcc tgcggacgat cgtcggtccg
      541 ccacccgcac gcgacacgcc ggagaatgtc cttttcctgt gcaactgttt cgtgaactgg
      601 ttcatcttcg gctcgaacct cttcgcgagg ggcgagctgg cgcgttcgct cgacctcctc
      661 gggatcgtac gtgaccggct gttacagatg gtgagggtgc tggaaggctc gacgctgcac
      721 tggttcaatt cctcgaagtt cctcgaacgc gagatctccg agacctcgta cagacggtac
      781 gtgtcgtgca ccgcccgcct cgacacagac gagcttcgcc gggcgtacct ctcagcctgg
      841 gagtggggca gggagctgat gccgtcgctc gccgacgggt acggcgtcgt cctgcctgcg
      901 gacctgctct tgcggcttga cgggcgcttc gctgagacct tccgcacggc ggaacaagag
      961 ggaccgtgaa ggagacggag aacccgtggc ggacgctcgg gtcgaagcgc gtctacgaga
     1021 acccctggat ctcggtccgc gaggacaggg tgatacgccc tgacggcgag cccggtatat
     1081 acggcgtcgt ccactacaag aacagagccg tcggcgtcct gccagtggag gacggccacg
     1141 tctacctggt agggcaatat cgctaccctc ttgcacacta ctcctgggag atacccgagg
     1201 gcggttgccc cgaggacgag gaaccccttc gcgcggccca acgcgagcta aaagaagaga
     1261 ccggcctcga agcgaggaac tggcggatgc tcggcgaggc atacctctcc aactccgtcg
     1321 ccgacgagca cgctgtgtgg ttcctcgcca caggactcgt gcccggcgaa caacagcccg
     1381 aaggtacaga ggtgatcggg gtgcgccgcg ttccggtaag cgaagccctc gccatggcga
     1441 tggacggcag gatcaccgac gccctcagcg ttctcgccct cacgacctac gccctggatc
     1501 aatccagagg caacgatggc taggatctcc tgctacttat tggaggcgcg atgaaggtct
     1561 gcgcgctcgc gggcggggtc ggcggcgcga agctcgccgc cggcttgcag gacgtgctgc
     1621 cggcgggtga tctggcggta gtcgtgaaca cggctgacga cttcgacctc tggggactcc
     1681 gcatctgccc cgacctcgac acggtcatgt acacgctcgc cgggatctcc aaccccgaga
     1741 ccggctgggg cct
//
LOCUS       DATAIR010000587         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_454963, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000587 DATAIR010000000
VERSION     DATAIR010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454963"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VHM69_13950"
     CDS             <1..623
                     /locus_tag="VHM69_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2741544.1"
                     /translation="ALQQMELAGEYPDIVIGCVGGGSNFGGVAFPFVRENLKNGKNTR
                     IVAVEPASCPTLTKGRYVYDFGDTAGTTPMMKMYTLGHSFVPSGIHAGGLRYHGDSPI
                     VSALVHEGLVEARAYAQNPTFEAGVTFARTEGIVPAPEVTHAIKATIDLALEAKEAGE
                     EKTILFNLCGHGHFDFSAYEQYLAGELVNLEHSDEEIEEAVAGIPG"
     gene            complement(662..1231)
                     /locus_tag="VHM69_13955"
     CDS             complement(662..1231)
                     /locus_tag="VHM69_13955"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2741545.1"
                     /translation="MESTKKDAVSGEAAELARAEVGEHEITNMLPADGKKPDAARPSP
                     EEPPRGIEVYPATTNRTVKGPIEYDRRPPTNGDHDPLWQNCGFYEKPIKERHAVHSMD
                     HGVVWITYRPDLPKQQLESLRPYGEENYVIVSPYPGQDAPVISTSWRVQLKLKGADDP
                     RLRKFVNQFRISELAPLSGNRCTSGVGDR"
     gene            complement(1538..2428)
                     /locus_tag="VHM69_13960"
     CDS             complement(1538..2428)
                     /locus_tag="VHM69_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX2741546.1"
                     /translation="MEKPRVVVVGDLLYDLLARVDGDVTLGTDTFTKIRGVAGGSGAN
                     AAAWLASAGVETHFVGRVGDDVFGGFLEGEMERAGVKAQLARDPSVATGKVFVLVDGA
                     GERTMITDRGAGENLSPEDLPESLFGKGHLHLSGYTLSGGSRRETARKALRLVRESGM
                     TFSVDPSSVPMLGDVGPERFLRWTSGADLCFPNLEEGSLLAGTEGPERIAERLLDYYS
                     GVVLKLGAEGAIYAGADGERSRVPAAPARVADTTGAGDALCAGFIEAWLAGAPADEAL
                     RRGVGLAARAVERVGGRPEG"
BASE COUNT          420 a          813 c          760 g          459 t
ORIGIN      
        1 aagcgttgca gcagatggag cttgcaggcg agtaccccga catcgtaatc ggctgcgtag
       61 ggggcggctc gaacttcggc ggcgtcgcct tcccctttgt ccgtgagaac ttgaagaacg
      121 ggaagaatac gcgcatcgtc gccgtcgagc ccgcctcctg tccgacgctc accaaaggcc
      181 gctacgtcta cgacttcggc gacaccgccg ggacgacccc gatgatgaag atgtacacgc
      241 tggggcactc gttcgtcccg agcggcatcc acgcaggagg gctgcgctac cacggcgact
      301 cccctatagt ctcggcgctc gtgcacgagg ggctcgtcga ggcgcgggcc tacgcccaga
      361 acccgacctt cgaggcaggg gtcacgttcg ccaggacgga gggcatagtg cccgcgcccg
      421 aggtgacgca cgcgatcaaa gccaccatag accttgcgct cgaagcgaag gaggccggag
      481 aagagaagac catcctcttc aacctctgcg gccatggcca cttcgacttc tctgcttacg
      541 agcagtacct cgccggggag ctcgtcaacc tggagcactc ggacgaggag atagaggagg
      601 ccgtcgccgg cataccgggc tgaggacagt tctcgcgtcc cgcgaattga tttattcgcc
      661 cttaccggtc gccgacgcct gaagtgcagc ggttgccaga gagtggcgcc agctcggaga
      721 tcctgaactg gttcacgaac ttcctgagac gcgggtcgtc tgcgcctttc agtttgagct
      781 ggaccctcca tgaggtgctg ataacgggtg cgtcctggcc aggataaggg ctgactatga
      841 cgtagttttc ctcgccgtaa ggacgtagag attcgagctg ctgcttcggc aagtcgggac
      901 ggtaggtgat ccagaccaca ccgtggtcca tgctgtggac ggcgtgcctt tccttgatgg
      961 gcttctcgta gaagccgcag ttctgccaca gcgggtcgtg gtccccgttg gtcggcggcc
     1021 tcctgtcgta ttcgatgggt cccttgacgg tacggttggt cgtggctggg tagacctcta
     1081 tccctctggg cggttcctcg ggcgaaggcc gcgccgcgtc aggcttcttc ccgtcggcgg
     1141 gtagcatgtt ggtgatctca tgttcaccaa cctcggcccg cgccagctcg gccgcctcgc
     1201 cggagacagc gtctttcttc gtactctcca ccacagtggt cttttgagcg gcgctctgtt
     1261 ccggctttcc tgaagcttcg tgtttcccgg aaccgttcga agaggttctc gacgattccc
     1321 caccacaaga cgccacgagg aggattagcg cggccgcaag taccatgatg gcgagcttcg
     1381 tcgagcggga acttcttccg tgctttccac tcggaccctc gacgttcaag aaatgcgacc
     1441 ttcctgtctg cttacggtaa gggtatctac ggacctggag taactatgga tgtaccggag
     1501 atcaccgcgg gcgcgccagc ccgatcaccg aaaacttcta tccctctggt cgaccgccca
     1561 cgcgctcgac ggcacgcgcc gcgagcccga cgccccgccg caacgcctcg tctgccggtg
     1621 caccggcgag ccaggcctcg atgaagccag cacagagggc gtctcctgcg cccgttgtgt
     1681 cggccacccg cgctggtgcg gcggggacgc gcgaccgctc cccgtccgcg cccgcgtaga
     1741 tggctccctc agcgccgagt ttcaggacga cgccggagta gtagtcgagg agcctctccg
     1801 cgatgcgctc cgggccttcg gtccctgcga gcagcgaacc ctcttcgagg ttagggaaac
     1861 acaggtccgc gccactggtc caccgtagga agcgttccgg acccacgtcg ccgagcatcg
     1921 ggactgacga ggggtctaca gagaaggtca tgcctgactc gcgcaccagg cgcagggctt
     1981 ttctcgccgt ctctctgcga ctgccgcctg agagggtgta gcccgagagg tgcaggtgtc
     2041 ccttgccaaa gagagattcg ggaagatctt cggggctgag gttctcaccg gcgccgcggt
     2101 cggtgatcat ggtccgctcc cctgcgccgt cgacgagcac gaagaccttg cccgtcgcca
     2161 cggaagggtc acgtgcaagt tgcgccttca cgccagctct ctccatctcc ccttcaagga
     2221 acccgccgaa gacgtcgtcc ccgaccctcc ctacgaagtg cgtctcgacg cctgcggagg
     2281 cgagccaggc ggccgcattc gcgccggaac caccggcgac gccccggatc ttcgtgaagg
     2341 tatctgtccc cagggtcaca tccccatcca ccctggcgag caggtcgtat agcaggtccc
     2401 cgactacgac gaccctcggc ttctccacgc ctcaggaccg tctggctgag ag
//
LOCUS       DATAIR010000588         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_39707, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000588 DATAIR010000000
VERSION     DATAIR010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39707"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VHM69_13965"
     CDS             complement(<1..1295)
                     /locus_tag="VHM69_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX2741547.1"
                     /translation="MKGSLLVAGTHSDAGKSVVVAGICRWLAREGVRVAPFKAQNMAL
                     NSFVTREGAEIGRAQAAQAAAAMVEPDAAMNPVLLKPSAGQTTQVVVRGKAWATANAM
                     SYSGMKKGLMPVVLESFEELRSRFDVIICEGAGSPAEINLRENDLANMGLARAADLPV
                     LLVGDIDRGGLFASLYGTLALLSPDDQSLVAGFLVNKFRGDTAVLAPGLNRMASLTGR
                     PFFGTLPWIRGLGLDGEDSLALDAPRPVKPPLGRDVLRVAVARLGRISNFTDFDALAH
                     EPGVSVRFTESAEEILAADLAVLPGTKATVDDVKLLRTRGLDRALAERADLGLPMLGI
                     CGGYQMLGGRIADGVESCEGEVVGLGLLPVETFFEEEKALGRPGGRASSFGDAVVSGY
                     EIHHGRVRRYGGEPLFDAGDETEGCLTGATLGTSWHGVF"
     gene            complement(1292..1999)
                     /locus_tag="VHM69_13970"
     CDS             complement(1292..1999)
                     /locus_tag="VHM69_13970"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX2741548.1"
                     /translation="MVSAYLRRGMAAGLLVGLLAGVFAFFVGEPILDRAIALEEAAHH
                     EEGHEEVFSRSTQKAGLFFATGLFGVTTGGVFGISYAFFRERLAAGSEFKRSIFLAGA
                     IFAGAFLIPFLKYPANPPTVGDPATIRERTAAYFTLVALSLLAIFLAWLAARALKSRG
                     INARNRRLMAGAGLVVVIGVLFLLLPAAPSTDGFPSGLLWAFRLSSFGTQLIFWAGLG
                     VLFGLLCERANRKGAVA"
     gene            complement(2008..2217)
                     /locus_tag="VHM69_13975"
     CDS             complement(2008..2217)
                     /locus_tag="VHM69_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741549.1"
                     /translation="MEKTLGRRQTSTLPLWSWLALALLLLALFVLLSANGALLAPLFG
                     QAAGAFDYLHEFAHDGRHLLAVPCH"
     regulatory      complement(2251..2432)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2494..3156
                     /locus_tag="VHM69_13980"
     CDS             2494..3156
                     /locus_tag="VHM69_13980"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2741550.1"
                     /translation="MQILLDFIGSIPNSPQAILGFVTNLLIAHGYLVVLFGAALDNFG
                     LPASGDIVLLAGGLFANSGHLALPLVMLSGFAGAIISDNAVYWIGRTGGRPLIYRILK
                     IRLLHFLVHERSLNRAERYFESHGGKTVFVGRFGPGLRSMTPLFAGVTRMKYYRFLPY
                     NVAAAVVWAVAYSLVGYVFGQYWSELLAIAKSLGYGVVALVVLALLAYLLRRRRVRNK
                     LM"
     gene            3324..>4163
                     /locus_tag="VHM69_13985"
     CDS             3324..>4163
                     /locus_tag="VHM69_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741551.1"
                     /translation="MGPSDNPEGRRRAEEPLRQSEERFRLLVEGVKDYAIFMLDEQGR
                     VSTWNKGARHIKGYSAEEILGQHFRIFYTEADIARSHPEEELRKAAAEGTYEEEGLRV
                     RKDGSRFWADVVITALRDEDGNLRGFAKVSRDITTRKEAEERERLLAQEQAARERATD
                     ILESISDAFYAVDEEWRFTYVNGKAEDLWGRPREELLGKKIWDEFPEAPGSEFHEQVR
                     RAAEERVTTAFETPSPVRGTWIAGRIYPTRAGLSVYFRDVTERRRTEEGLGFLAEASE
                     VLSS"
BASE COUNT          786 a         1316 c         1361 g          700 t
ORIGIN      
        1 tcgaagacac cgtgccacga cgtaccgagc gtcgcccctg tcaggcagcc ctccgtttca
       61 tcgccggcat cgaaaagagg ctcgccgcca taacgcctga ctcttccgtg gtggatctcg
      121 tatccagata ccaccgcatc cccgaagctg gaagcccgtc ccccgggacg tcccagagcc
      181 ttttcttcct cgaagaacgt ctcgacgggc aggagcccga gaccgaccac ctctccttcg
      241 cagctttcca cgccgtccgc gatgcgtccg ccgagcatct ggtagccgcc gcagataccg
      301 agcatcggca ggccgagatc agcccgttcg gcgagggcgc ggtcgaggcc gcgggtgcgg
      361 agtagcttca cgtcatctac cgtcgccttc gttcctggca ggacggcgag atccgcggcc
      421 aggatctcct ccgccgactc cgtgaagcgc acagagacgc cgggctcgtg ggccagggcg
      481 tcgaagtccg tgaagttgct gatgcgcccg aggcgcgcga cggcgactcg cagaacgtcg
      541 cggccgagcg ggggcttcac cggacgcggc gcatccaggg ccagcgagtc ctccccatcg
      601 aggccgaggc cacgaatcca gggcagcgtc ccgaagaaag gacggcccgt gagagaggcc
      661 atccggttga gaccgggggc caataccgcg gtatcccccc tgaacttgtt gacgaggaag
      721 cctgcgacga gcgactggtc gtcgggcgag aggagggcga gcgtcccgta gaggctcgcg
      781 aagaggccgc cgcggtcgat gtccccgacc aggaggacgg ggaggtccgc agcgcgggca
      841 agacccatgt tggcgaggtc gttctcacgg aggttgatct ccgccgggct cccggctccc
      901 tcgcagatta ttacgtcgaa acggctacgt agctcttcga acgactcgag cacgacgggc
      961 atcaggccct tcttcatccc actgtagctc atggcgttcg ccgtcgccca ggctttgccg
     1021 cgcactacga cctgcgtggt ctgtccggct gaaggtttta gaagcacagg attcatcgcg
     1081 gcatccggtt cgaccatcgc cgcggcggcc tgtgcggcct gggcgcgccc gatctcagcg
     1141 ccctcccgag tcacgaacga gttgagcgcc atgttctggg ccttgaacgg cgctaccctc
     1201 acgccctcgc gggccagcca gcggcagatg ccggccacga ctacgctctt ccccgcgtcg
     1261 gagtgggtcc cggccacgag tagagaacct ttcaagcgac agcgcccttc ctgttggccc
     1321 gctcgcacag caggccgaac agtaccccga gcccggccca gaagatcaat tgtgtaccga
     1381 aggacgagag cctgaaagcc cagagcagcc ctgacgggaa cccgtcagtg gaaggtgcgg
     1441 caggcaagag caggaacagg acgcctataa cgaccaccag accggctcct gccatcagac
     1501 gacggtttcg ggcattgatc cctcgcgact tcagtgcgcg ggcggcgagc caggcgagga
     1561 atattgccag cagcgagagg gcgacgagcg tgaagtaggc cgctgtccgc tcccttatcg
     1621 tggcgggatc gccgacggtt ggagggttgg cgggatattt gaggaagggg atcaagaacg
     1681 caccagcgaa gatggcgccg gcgagaaaga tactgcgctt gaactcgctg ccggcggcga
     1741 gccgttcgcg aaagaaagcg taggagatgc cgaacactcc cccggtcgtc accccgaaga
     1801 ggcccgtggc gaagaaaagt ccggccttct gggtcgagcg ggagaagacc tcctcgtggc
     1861 cttcctcatg atgggcggcc tcttccagcg ctatggcccg gtcaaggatg ggctctccga
     1921 cgaagaaagc gaacacgccg gccaggaggc cgacgagcag cccggcggcc atgccgcggc
     1981 gcaggtaggc cgagaccacg ggcgtctcta gtggcaggga acggcgagca ggtgtcgccc
     2041 gtcatgggcg aactcgtgca ggtagtcgaa ggctcccgcc gcctgcccga agagcggagc
     2101 gagcaaagcc ccgttggcgg agaggaggac gaaaagcgcg agtagcaaca aagcgagggc
     2161 cagccaactc cacaggggca gggtagaagt ctgcctccgt ccgagggtct tctccatagg
     2221 tgcctccttc tcgggggtcc tcgccccggt tcggggttac gggcgaaagg tcagtctcct
     2281 gactcccggc tctgtgctgc cccggccttc ccgctgcgct tgcggtggct tagtcgggtt
     2341 cgctccccgg ttacagtggc gggaccgtgc cggactcgca ccggcttcct ggaccccttc
     2401 gcccctcaat tgcctcaggg cagcatagcc gggctgccag cacgcgtcaa ccgtttcgcc
     2461 gcataccttg gcgtggtata aaccgtccgg cgcatgcaga ttcttctgga cttcattgga
     2521 tccatcccga actcccccca ggccatactg ggtttcgtga cgaaccttct catagcccac
     2581 ggctacctcg tcgtactctt cggggcggct ctggataact tcggtctccc cgcctcggga
     2641 gatatagttc tactggcagg cgggttgttc gccaacagcg gacacctcgc gctcccgctg
     2701 gtgatgctaa gcggcttcgc cggggccatc atctccgaca acgccgtgta ctggatcggg
     2761 cgcacgggag gcaggcccct gatctaccgc atcctcaaga ttcgtttgct ccacttcctg
     2821 gtccacgaga ggagcctcaa cagggcggag cgctacttcg agtcgcacgg cgggaagacg
     2881 gtcttcgtcg ggcgtttcgg gccaggcctg cgcagcatga ccccactctt cgcaggagtt
     2941 accaggatga agtactacag atttctcccg tataacgtgg cggcggcggt cgtttgggcc
     3001 gtggcctact cgctggtcgg ctacgtcttc ggccagtact ggagcgagtt gctcgccatc
     3061 gcgaagtctc tcggctacgg cgtcgtcgcc ctcgttgtcc tggcgctgct cgcgtacttg
     3121 ctcagacgac gccgggtcag aaacaagctg atgtaggtac aaacggtgtc cgcgcccgga
     3181 aaggcttcac gctcttgtcc gagaagccta cttccgcaga ccgtcgatta tccggtcccg
     3241 gcgattccct gagagcaata aatctgcgaa aatgacttgg ctaaaacttc tcctgagttc
     3301 gccgaggtag ttcaaccata gacatgggac cctccgacaa tccagaaggg cgcaggcggg
     3361 ccgaggaacc cctgcgccag agcgaggagc gcttcaggct gctggtggag ggcgtcaagg
     3421 actacgccat cttcatgctc gacgagcaag gtcgcgtgtc gacctggaac aagggagccc
     3481 gacacattaa gggttacagt gccgaggaga tactgggaca gcactttcgt atcttctaca
     3541 ccgaagcgga catcgcgcgt agccaccccg aggaggagtt gaggaaggcg gctgcggaag
     3601 gaacctacga ggaggagggt ctcagggtac gcaaggacgg ttctcgtttc tgggctgacg
     3661 tagtcatcac ggccctgagg gatgaggatg gcaacctccg cggcttcgcc aaggtgtcga
     3721 gggacattac cacacgcaag gaggccgaag aacgcgaacg cctgctggcc caggagcagg
     3781 cagccaggga gagggccacg gacatcctgg agagcatcag cgacgccttc tacgccgtcg
     3841 atgaggagtg gcgcttcacc tacgtcaacg gcaaggctga ggatctatgg gggcgtccga
     3901 gggaagagtt gctggggaag aagatctggg acgagttccc cgaggctccg ggctccgagt
     3961 tccatgagca ggtcaggcgg gcggccgagg agcgcgtgac cacagcgttc gagacaccgt
     4021 ctcctgtgcg ggggacgtgg atcgcgggac gaatctaccc cactcgggcg ggactttccg
     4081 tctacttccg ggacgtgaca gagcgcaggc ggaccgaaga aggtctgggc ttccttgccg
     4141 aagcgagcga ggtgctatct tcc
//
LOCUS       DATAIR010000589         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_40152, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000589 DATAIR010000000
VERSION     DATAIR010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40152"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VHM69_13990"
     CDS             <1..1604
                     /locus_tag="VHM69_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2741552.1"
                     /translation="FAELLGYGADELLDMKIYKFIAHEVDDIDLNVQRHLIEEKRFVG
                     ERKYLRKDGRLLEVEVSATLIPYGGKEVVCCVVRDVTGRKALEEQLARQAFHDSLTDL
                     PNRALFLDRLGHTLARARRGDGQAAVLFVDLDDFKIINDSLGHESGDLLLAEVAERLK
                     SCVRPGDTVARLFGDEFAVLLESPTAGDQAQMVAARVLERLQAPFDLNGREVFASASI
                     GITDCEPVRSIDQDSKQGRPEDVLRRADLAMYTAKRRGKNGYEVFSPSMNTRVTERME
                     VENQLRRAVEREEFIAHYQPIIDLKSGAIRGVEALARWEHPERGVIVAEEFAQIVEET
                     GLIRPVGRRVFEAACRTAKEWCGLHPDRSFLMSVNFSASQFVHQADLIPKVLNDTGLD
                     PSALVIEITERAVMDDAEFAIGKLQRMKDLGVSFIIDDYGTGYSCLKYLKLMPVDSLK
                     IDGSFIAGLGKDWGDTAIVSGTIDLAHALELTVVAEGVETAGQLEQLRELECDLVQGY
                     YLSEPLPEGEMRELLTADPGRWPYR"
     gene            1691..>3155
                     /locus_tag="VHM69_13995"
     CDS             1691..>3155
                     /locus_tag="VHM69_13995"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX2741553.1"
                     /translation="MTVDNGRPARRFRSRDWFDDPHRRDQSALYLERFMNYGITPDEL
                     RSERPVVGIAQSGSDLSPCNRHHVELASRVRDGIRDGGGIPMEFPVHPINENCRRPTA
                     ALDRNLFYLGLVEILYGYFMDAVVLTTGCDKTTPAAIMAASTVDIPAIVLSGGPMLDG
                     YFEGELVGSGMAIWKSRRRLAAGQIDEEEFFETALASAPSAGHCNTMGTASTMNALAE
                     VLGLSLPGCAAIPAPYRERGQMAYDTGKRIAEMAYEDLRPSKILTREAFLNAIVATTA
                     IGGSTNAQPHIMAMARHAGVEIEPSDWEQGFDVPLLVNMQPAGKYLGERFHRAGGVPA
                     VVNELLRAGKIDGDAMTVTGKTLAESVAGRESVDREMIAAHDEPLQEHAGFLVLSGNL
                     FDFAIIKTSVISDEFRDRYLSDDGVFEGRAVVFDGSDDYHHRINDPDLNIDERSILVI
                     RGAGPIGWPGSAEVVNMQPPDALLKRGINTLPTIGDGR"
BASE COUNT          641 a          947 c         1023 g          544 t
ORIGIN      
        1 ccttcgctga actgcttgga tacggcgccg acgagttgct tgatatgaag atctacaagt
       61 tcatcgccca cgaagtggat gacatcgacc ttaacgtcca gcgtcacctg atcgaggaga
      121 agcgcttcgt cggcgaacgc aagtaccttc ggaaggacgg gaggttgctc gaagtcgagg
      181 taagcgcgac cttgatcccc tacggtggta aagaggtcgt ttgctgcgtg gtccgcgatg
      241 ttaccgggcg caaggccctc gaggagcagc tagcccgcca ggctttccac gactcgctca
      301 ccgatctgcc caaccgggct ttgtttctgg acaggctcgg gcacaccctc gcgcgggcgc
      361 gcagggggga cggccaggct gccgtgctct tcgttgacct ggacgacttc aagatcatca
      421 acgactccct gggtcatgag agcggagacc tgctactcgc cgaggtcgca gaacgcttga
      481 aatcttgcgt gagaccggga gatacggtag cccgactctt cggggatgag ttcgccgttc
      541 tgctcgagtc gccgaccgcc ggcgaccagg cccagatggt cgccgcacgg gttctcgaga
      601 ggttgcaggc gccgttcgac ctcaatggaa gagaggtctt cgcgagtgca agcattggta
      661 tcacggactg cgaacctgtc aggagcatcg atcaagactc gaagcaggga cggccggaag
      721 acgtcctgcg ccgggcggac ctggcaatgt acacggcgaa gcggcgcggc aagaacggtt
      781 acgaggtgtt cagcccgagc atgaacacca gggtgacaga acgtatggag gtggagaatc
      841 agctcagacg cgcggtagag cgcgaggagt tcatagccca ctaccagccg atcatcgacc
      901 tcaaaagcgg cgctatcagg ggcgtggagg cgctggcgcg ctgggagcat cctgaacggg
      961 gggtgatcgt cgctgaggag tttgcccaga tcgtggaaga gacaggcttg atccgtcccg
     1021 ttgggcgaag ggtcttcgag gcagcatgcc ggactgccaa agagtggtgc ggactacatc
     1081 cagacaggtc attcctgatg agcgtgaact tctcggcgag ccagttcgtc caccaggctg
     1141 acctgatccc caaggttctc aacgataccg gattggaccc cagcgccctg gtcatagaga
     1201 tcaccgagcg ggccgttatg gacgacgcgg agttcgctat aggcaaactg cagaggatga
     1261 aagatctcgg ggtgagcttt atcatcgacg attacggaac ggggtactcc tgcttgaagt
     1321 acctgaagct gatgccggtc gattccctga agatcgacgg ctccttcatc gccggactcg
     1381 gcaaggattg gggagacacg gcgatagtgt ctgggacgat agatctggcg cacgccctcg
     1441 aactgacggt ggtcgcagag ggcgtggaga ccgcggggca gctcgaacag cttagagagc
     1501 ttgagtgtga tctggtccag ggctactatc tctccgaacc tttgccggaa ggagagatgc
     1561 gagaactctt gactgccgac cctggccgtt ggccgtaccg gtaaccatgg cactcacgtg
     1621 aacgatccgg aggatttgtt gaagataatt gacgtgagtg ccgcgaccgt cgagacgaac
     1681 aaggagagac atgaccgttg acaatggccg ccccgcgcgc cggttccgct cgcgcgactg
     1741 gttcgacgac ccccaccgca gagaccagag cgcgctatac ctggaacgct ttatgaacta
     1801 tgggatcaca cccgacgagc tgcgctcgga gagacccgtc gtcggcatcg cccagtcggg
     1861 cagcgacctc tcgccatgca acaggcacca cgtggaacta gcgagccgtg tgcgcgacgg
     1921 gatacgcgac ggcggcggta tcccgatgga gttcccggtc catcccatca atgagaattg
     1981 ccgcaggccg accgccgcgc tcgaccgcaa cctcttctat ctggggctgg tcgagatcct
     2041 ctacggttac ttcatggacg ccgtggtgct caccacgggc tgcgacaaga cgactccagc
     2101 ggcgatcatg gccgcctcga cggtggacat accagcaatc gtgctctcgg gcgggcccat
     2161 gctcgacggc tacttcgagg gcgaactggt gggttctgga atggctatct ggaagagccg
     2221 ccggaggctc gccgcgggcc agatcgacga ggaggagttc ttcgagacgg cccttgcctc
     2281 cgcgccttcg gcaggccact gcaacacgat ggggacggcc tctaccatga acgccctggc
     2341 cgaggtgctc gggctctcgc tgccaggatg cgcggccata cccgccccct accgcgagcg
     2401 cgggcagatg gcctacgaca cggggaagcg catcgccgag atggcatacg aggacctgcg
     2461 cccgtcgaag atactgacgc gcgaggcttt cctgaacgcg atcgtcgcca ccacggccat
     2521 aggcggctcg accaacgccc agccgcacat catggcaatg gcgcgccacg ctggcgtcga
     2581 gatcgaaccc tccgactggg agcaaggctt cgacgtgcct ctgctcgtca acatgcagcc
     2641 agcgggcaag taccttggcg aaaggttcca ccgcgcaggc ggcgtgcccg ccgtggtcaa
     2701 cgagctgttg cgggccggca agatcgacgg tgacgcgatg accgtcaccg gcaagacgct
     2761 ggccgagagc gtggcggggc gcgagagtgt tgaccgggag atgatcgcgg cccacgacga
     2821 gccgttgcag gagcacgcag ggttcctcgt cctctcgggc aacctcttcg acttcgcgat
     2881 cataaagacg agcgtgatct ccgacgagtt ccgcgaccgc tatctcagtg acgatggcgt
     2941 gttcgaaggt cgagcggtcg tcttcgacgg ctcggacgac taccaccacc gcatcaacga
     3001 ccctgacctg aacatcgacg agcgatcaat tctggtgatt cggggcgctg gacctatcgg
     3061 gtggccaggc tctgccgagg tcgtcaacat gcagccacct gacgcactgc tgaagcgtgg
     3121 gatcaacacc ctgcccacca taggcgacgg caggc
//
LOCUS       DATAIR010000590         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_64337, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000590 DATAIR010000000
VERSION     DATAIR010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64337"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VHM69_14000"
     CDS             complement(<1..980)
                     /locus_tag="VHM69_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741554.1"
                     /translation="MGVLQSPQPPPIESILTALLNEIDTIPDDFVLVLDDYHVIDGSA
                     VDEALSFVVEHLPPGMHLVIATREDPHLPLARLRGRGQLSEVRATDLRFAPDEAAEFL
                     KVVMGLSLSAQDIATLETRTEGWIAGLQMAALSMQGLEDATSFIKSFTGTHHFVLDYL
                     VEEVLKQQPKSIQTFLLRTSILGRLCGPLCDAVVLDPAMSGQETLEYLERANLFLVPL
                     DNERRWYRYHHLFADLLRQRLHQSAASSTEDERESVAELHLRASIWCEDNGLEVEAFQ
                     HAAAADDVERAARLVEGEGMPLHFRGAIAPVLSWLESLSTTELDATPSLWV"
     gene            complement(1435..1614)
                     /locus_tag="VHM69_14005"
     CDS             complement(1435..1614)
                     /locus_tag="VHM69_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741555.1"
                     /translation="MLRERRPAESLSEATGGRPELSLRYRELLEPLGFAREVSSLFAD
                     SGGPLEDAPGRPWAA"
     gene            2181..>2732
                     /locus_tag="VHM69_14010"
     CDS             2181..>2732
                     /locus_tag="VHM69_14010"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2741556.1"
                     /translation="MDTSHTGTKELCVRAISIMADGTLEDFEAVVHPDARNREDVDEP
                     PSSRGRGPGAFYATALWLRDAYAELHWDIHDVVAEDDLVVVHTTMSGRHVRTFVGYDA
                     EARPAQAFPPTGRRFATTQTHWFRVAEWKVIEHWANRDDLGTAMQLGWTPPSPLYLVR
                     MVLATSRARREARQATRGGRGESN"
BASE COUNT          543 a          866 c          847 g          476 t
ORIGIN      
        1 acccataatg agggcgtagc atccagttcc gtcgttgata gcgactccag ccagctcaac
       61 actggggcta tcgcgcctct aaagtgcagg ggcatcccct ctccttcaac caggcgcgcg
      121 gcacgctcca catcatcggc ggcagcagcg tgctgaaatg cttcaacctc cagaccattg
      181 tcttcacacc atatgctcgc acgtagatgc aattcggcca cgctctccct ctcatcctct
      241 gtggacgagg cggcgctctg gtgcagtcgc tgccgcagca aatctgcaaa gagatggtga
      301 taccgatacc aacgccgctc gttgtccagc gggacgagga acaggttggc tcgttcgaga
      361 tattccaggg tttcttgccc tgacattgcg gggtcgagca ctacggcatc acaaaggggg
      421 ccgcacaggc gcccgaggat agacgtgcgc agcaagaaag tctggatgct tttgggctgc
      481 tgcttgagga cttcttcaac cagataatcc agcacgaaat ggtgggtgcc ggtgaaagat
      541 ttgatgaagc tggtggcgtc ttctagcccc tgcatggaga gtgcagccat ctgcagtccg
      601 gcgatccaac cctcagtacg tgtttccagc gtggcgatgt cttgcgctga gaggcttaga
      661 cccatcacca ctttgagaaa ttcggcggct tcgtcaggag caaaacgtaa gtctgtggcg
      721 cgcacttcac tcaattggcc cctgccgcgt aaccgggcca ggggtagatg tggatcctca
      781 cgggtggcga tgaccaggtg catacctggc ggcaagtgct cgaccacgaa gctgagggct
      841 tcgtcaaccg cactgccatc gatgacgtgg tagtcatcga ggacgagaac gaaatcatcc
      901 ggaatggtgt caatctcatt gagcagggcc gtaagaatcg attcgattgg tggtggctga
      961 ggggattgga gcacacccaa cacgccttct ccaatgttcg gggcaatcgt ctgcaaagca
     1021 gcgacgaggt aagctagaaa gcgcgtggga tcgctatccc cttcgtccag cgacagccag
     1081 gcggccggcc gctggcaacc ggcgacccat tcgccgagga gcgtagtctt accgaagccg
     1141 gcgggggcgg agacgagggt cagtttgcgg tgcagaccct ggtcgagccg tccaaccagg
     1201 cggggccgga gaacgactct aggccgaggt ggggggatgt agagcttagt ggttaggacc
     1261 ggcgcagaca tagaccaatt ttagaacacg cacatcctcg gcaccctcca aaccgcttcc
     1321 ctccgaatac cccatcacgt agctggaacc gctttacgtt acggcccacc gcttcgacct
     1381 gccgagctac gtcagcccga gtatgttgtg tcctggtctt gaagcctccg actatcaggc
     1441 agcccaaggg cggccaggag catcctcaag tggtcccccg ctgtcggcga agaggctgct
     1501 cacctcgcgg gcgaagccca ggggctctag gagctcgcgg tagcgcaagg agagctccgg
     1561 ccttccgccg gtagcctcgg agagcgactc cgccgggcgc ctttcacgga gcatggaggc
     1621 gatcttgccg agactctcct cgaagtagac gcggtcgaag aagacgacgc cgtccttggc
     1681 ccaagccctc ggtgacgccg tgagtcatga ggctggtcgc cgggtccacc gtcgaggcgc
     1741 agtacgcgtc gaaggggacc gcgcacccta ggctctgcgc ggcccgtctc agcaactttg
     1801 gccccaatct cgagatcgac gcgtgcttga cctccgcgaa ggcccgctct ttctatcgag
     1861 cggaaacact gcggattatg ctcgtccctc cgatccggcc gtcatcgccc cgccggtgag
     1921 cgggccgtgc gcggcttcga cctcgacgca gctctagatg ctttcgttgc aacaacgatg
     1981 gtagcgcggg acgttgcggg cagcatcatc cggatgggcg gttttgtcgc gcccgaaaga
     2041 tccagccctc aaccgacgac gcgcctcccg aaaatggcag atttctgcaa tgggcgcagc
     2101 gccccggcac tgctaccctc ccacagaaca ggtcgaacga gaaacgggcg aactgaggat
     2161 gggtacggaa aggagcagtg atggacactt cccacaccgg cacgaaggag ctctgcgtcc
     2221 gcgcgattag catcatggcc gacggcactc tcgaagactt cgaggccgtc gttcaccccg
     2281 acgcccgcaa ccgcgaggac gtcgacgagc cgccgtccag ccgcggtcgc ggacccgggg
     2341 ccttctacgc caccgccctg tggctgcgcg acgcctacgc cgagctacac tgggacatcc
     2401 acgacgtggt tgccgaggac gacctcgtcg tggtgcacac cacgatgtcc ggccggcacg
     2461 tgcgaacctt cgtcggctac gacgccgaag cgcgccccgc ccaggccttc ccgccgacag
     2521 gcaggcggtt cgctaccacc cagacgcact ggttccgcgt agcggaatgg aaggttatcg
     2581 aacactgggc caaccgggat gacctcggca ccgcgatgca gctcggctgg accccgccgt
     2641 cgccgctgta cttggtgcgg atggtcctgg cgaccagccg ggcccgccgg gaggccagac
     2701 aagccacgcg cggtggacga ggagaaagta ac
//
LOCUS       DATAIR010000591         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_455472, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000591 DATAIR010000000
VERSION     DATAIR010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455472"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1637)
                     /locus_tag="VHM69_14015"
     CDS             complement(57..1637)
                     /locus_tag="VHM69_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2741557.1"
                     /translation="MSELTGLGVLFIGPDGDPLSARDGFGEPVAPGEAYRWLGLDDGI
                     VERAARSPTGQGRLVPRKGPRYIAVFPLLDVDGQMQGVMVYTVPQQGLDQTLAYLRYG
                     ILGAILASVLLAGLGSLLLTRQITLPLSETRDAAIRFASGDYKTAVPVTSNDELGEMA
                     RAFNYMAEEIEHYVGEIQEQKSRLEAVLEASPEAVVAADPEGRVTMVNPAAAGMLGIL
                     ATDYGRTLEEIGVPEEVSWCLTEAAKTGVAVREFESEEKTFWAYAARMDPNGGGNGAA
                     AILAVRDITEHRSLEQAKTAFVSDFSHELRTPLTTIQSAVGLLERARERLDPLEQRAL
                     ELADQELQRIRGMIEELLTLAQMDSWRYSLEVGPANLSNVVENAIESLESKAERFGIE
                     VYFEDREERRGVCDAPKLYHVFLNLLDNAIKYSDPGDRVDVLFEEDDSTVAVAVRDTG
                     FGIPQEDLEQLFERFYRVNKDRSRATGGSGLGLAIAKEIVELHGGSLDVESEVGVGST
                     FTVTLPKIPHTRSTSYAM"
     gene            complement(1950..>2496)
                     /locus_tag="VHM69_14020"
     CDS             complement(1950..>2496)
                     /locus_tag="VHM69_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741558.1"
                     /translation="VILDVMLPRASGFEVLQKIREQSDVPVVMLTAKSQSVDKVVGLE
                     LGADDYITKPFDTKELLARIKAILRRFGRQEGMGRDGVVKLGALELDPTGYTVSKEGQ
                     PLELTPTEFKILALLMRRPGQAFTRTQISDAVSTGQHYLASRYIDVHISRLRGKVERD
                     PSKPEIIQTVPSVGYRAARAS"
BASE COUNT          412 a          816 c          756 g          512 t
ORIGIN      
        1 gtaaaaccag cgccatcacc ttcaagacgc gccacggtcg cgggcggcgc gtgggatcac
       61 atcgcatagc tcgtggagcg ggtgtggggg attttcggga gcgtcacggt gaacgtcgag
      121 ccgacgccga cctcgctctc cacgtccagg ctcccaccgt gcaattcgac gatctccttc
      181 gctatggcga gcccgaggcc gctgccgccg gtcgccctgg agcgatcctt gttcaccctg
      241 tagaaccgct cgaagagctg ctccaggtcc tcttgcggga tgccgaatcc tgtgtcccga
      301 acagcgacgg ccaccgtcga gtcatcctcc tcgaacagta cgtccacccg atcgccggga
      361 tctgagtact tgatggcgtt atcgagcagg ttgaggaaga catggtagag cttcggggcg
      421 tcacatacgc ccctgcgttc ttcccggtct tcgaagtaga cttcgatacc aaacctctcg
      481 gctttggatt ccagggattc gatggcgttc tctacaacgt tgctgaggtt ggctggcccg
      541 acttcgagtg agtaacgcca cgagtccatc tgggccaggg tgagaagctc ctcgatcatg
      601 ccgcgtatac gctgcaattc ctggtcggcc agctcgagcg cgcgttgctc cagcgggtcg
      661 agcctctccc gcgcccgctc cagcaggccg acggcgctct ggatggtggt cagcggcgtg
      721 cggagctcgt gcgaaaaatc cgagacgaag gctgtcttgg cctgctccag ggagcgatgt
      781 tcggttatgt ccctcaccgc caggatggct gccgccccgt tgcccccgcc gttggggtcc
      841 atcctcgccg cgtaggccca gaacgttttc tcctccgact cgaactccct cacggcgaca
      901 ccggttttcg cggcttccgt gaggcaccag gacacttctt cggggacccc gatctcctcc
      961 agcgtgcgcc cgtagtccgt ggcaaggatg ccgagcattc cggccgccgc cgggttcacc
     1021 atggtcaccc gtccttccgg gtcggccgcg accacggctt ccggggaggc ttcgaggact
     1081 gcctccaggc ggcttttctg ctcctggatc tctccaacgt agtgctctat ctcttccgcc
     1141 atgtagttga aggcgcgggc catctcgcca agctcgtcgt tgctcgtgac gggcactgcg
     1201 gtcttgtagt cgccagaggc gaacctgata gcggcgtccc tggtctcgga gaggggaagg
     1261 gtgatctggc gcgtcaacaa taggctcccg agcccggcca acagcacgct ggccaggatg
     1321 gccccgagta tcccgtaacg gaggtaggcg agggtctggt ccagtccctg ctgcgggacc
     1381 gtgtagacca tgacaccctg catctgcccg tccacatcca aaagcgggaa taccgctatg
     1441 tagcgtggcc ccttccttgg cacaagcctg ccctggcccg ttggagacct ggcggcccgc
     1501 tccacgatcc catcgtcaag gcccagccat ctgtacgctt cccccggcgc gactggttca
     1561 ccgaaaccat ccctggcgga gaggggatcc ccgtcaggcc cgatgaagag cacgccaagc
     1621 cccgtcagtt cggacacaga cctgaccagc tcccccgcat aagtatccgg ggctggaagg
     1681 gtctgatcct ctgggttgcg gagaccttcg atgaactctc cggcgcccgc gttggcagtc
     1741 tgggcgcgct gggcgaggag ttcgctctcg gtgttaacca gacggtcctg ggcgaaatag
     1801 tagaggacca gtcctataac caggctggtg acggctgaga tcccgaccag ggcgacgaat
     1861 atcctggccc tgaagctcgg ccttcgaaag aattcgttct tgcttttgtt gtctacgtct
     1921 cgcctccgct gcttcctcga atagttctct caggaggctc gcgcggccct gtagccaacg
     1981 ctcgggaccg tctgaatgat ctccggtttc gatgggtcgc gctcgacttt gccccgcaat
     2041 cggctgatgt gaacgtctat gtagcggctg gcaagatagt gctgccccgt ggagactgcg
     2101 tcggagatct gggttctcgt gaatgcctgt cccggacgcc gcatgagcag ggcgagtatc
     2161 ttgaactccg tgggcgtgag ttcgaggggc tggccttcct tgctgaccgt ataccctgtc
     2221 gggtccagct ccagcgcgcc gagcttgacg acgccgtccc tgcccatccc ctcctgacgc
     2281 ccgaaccggc gcaggatcgc ctttatgcgg gcaagcaact ccttggtgtc gaagggcttg
     2341 gttatgtaat cgtcagctcc cagttccagg cccacaactt tgtccacgga ttggctcttg
     2401 gcggtgagca ttaccacggg cacgtcgctt tgctcccgga tcttctgcaa cacctcgaac
     2461 ccgctggcgc gtggcagcat cacgtcgagg atcacg
//
LOCUS       DATAIR010000592         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_64417, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000592 DATAIR010000000
VERSION     DATAIR010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64417"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VHM69_14025"
     CDS             complement(<1..443)
                     /locus_tag="VHM69_14025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2741559.1"
                     /translation="MPRGEQTRNLRCGHALKADLVSGLLPIREDGRMLLLQRPTGTWE
                     PPAGRLQPGESFEDGAVRELYEETGILVSPQRIIATWVGEAPSGGRLASVTYAGRTGG
                     GEVRLSDEHLDHRWVTIEEWMSLPSWWSRENIRRVPVREIRETPVP"
     gene            complement(489..1214)
                     /locus_tag="VHM69_14030"
     CDS             complement(489..1214)
                     /locus_tag="VHM69_14030"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX2741560.1"
                     /translation="MEHRPYPVHAGSWAMGMSWRNLLFMHWPVEEDALRPLVPPALSL
                     DTFEGSAWLGVVPFRMTGVRPRFLPAVPRLSDFPEINLRTYVTAEGRSGVWFFSLDAH
                     NPLAVRVARATFHLPYFDAEMSCHASGDEVDYRSVRTHRDAPRAEFAARYRPVGDPFE
                     SGRGTIENFLTERYCLYSADRKGNVRRGEVHHQLWPLQPAEAEVHTLAMTQQIGVELP
                     DTSPLLHFARRLDVLAWLPRRIF"
     gene            complement(1304..1942)
                     /locus_tag="VHM69_14035"
     CDS             complement(1304..1942)
                     /locus_tag="VHM69_14035"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX2741561.1"
                     /translation="MRAAVFLNGDPDSRGLIRAVAKRADLILAADGGARYALDAGIFP
                     DLVVGDMDSLGEDLAREIEERGASLERHPVQKDKMDGHLAVIAARERGATEVDFLCAV
                     GGKLGAIFAVPHILLVAGRIGLLATVVADWGRMFVVEAGSRAVQGAPRDSISIFPLSG
                     TATGVTLEGMAYPLEDAVLEPADTLGFHNELTGREARVSVRDGALLVVHETT"
     gene            complement(1944..2501)
                     /gene="thiT"
                     /locus_tag="VHM69_14040"
     CDS             complement(1944..2501)
                     /gene="thiT"
                     /locus_tag="VHM69_14040"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEX2741562.1"
                     /translation="MNAFRDTRVLTEAAIAVALAFVLGLVVLFKMPFGGTVSLEMVPL
                     ILLSLRQGWKVGIVAGVAYGFLDLAIDPFLVHPLQVLLDYPLAFGALGLAGFFRPTVR
                     GAILGTTVAVLARFVCHFLSGVVFFASYAPEGWNVYLYSAAYNAAYLAPSLGIALVVV
                     VVLLKALEGAQPSPRQLRYRHSATS"
     regulatory      complement(2536..2637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2633..>3038)
                     /locus_tag="VHM69_14045"
     CDS             complement(2633..>3038)
                     /locus_tag="VHM69_14045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2741563.1"
                     /translation="DVVLVRQWRQPLGAFTLELPSGAVDDGEEPEQAARRELFEETGY
                     RAEGLEHLTSVHTSTGRSTEVCHLFRCAAARDERGPRPEPTEFIQVVELPFQEAIEGI
                     REGSITDAATVLGLLWSACGGVDTRSTLHWEQ"
BASE COUNT          553 a         1023 c          950 g          512 t
ORIGIN      
        1 ggcacgggag tctcgcgaat ctcccgcacg ggtacgcgcc ggatattctc gcggctccac
       61 cagctcggca acgacatcca ctcttcgatg gttacccacc tgtggtcgag gtgctcgtcg
      121 gagagcctga cctctccccc acctgtccgc cctgcgtagg tgaccgaggc caggcgtcca
      181 ccgctaggcg cctcgccgac ccaggtggcg attatgcgtt gcggtgagac gaggatgccc
      241 gtctcctcgt agagctcgcg gacggcccca tcctcgaagc tctcgcccgg ctgcaggcgt
      301 cctgccggcg gctcccatgt ccccgtcgga cgctggagca gaagcatgcg gccgtcctcc
      361 ctgatgggca agaggccgga gacgagatcc gccttcaagg cgtgcccgca gcgaagattt
      421 ctggtctgct ccccacgcgg caagtatatg gctcgtaggg gctgttcgcg aacggccccc
      481 tgtggacctc agaagatcct tcgcggcagc caggcgagaa cgtccaggcg tctcgcgaag
      541 tgaaggagcg ggctcgtatc gggaagctcc acgcctatct gctgggtcat cgcgagcgtg
      601 tgtacctcgg cctccgcggg ctgcagtggc cagagctggt ggtggacttc gccgcggcgg
      661 acgtttccct tcctgtccgc gctgtacagg cagtagcgct cggtcaggaa gttctcgatg
      721 gtcccgcgtc cgctctcgaa ggggtctccg acagggcggt aacgggccgc gaactcagcg
      781 cgcggcgcgt ccctgtgtgt cctgacgctc ctgtagtcga cctcgtcgcc cgaggcgtgg
      841 caggacatct cggcgtcgaa gtaaggcagg tggaaggtcg cccgggctac tcggacggcc
      901 aaagggttgt gggcgtcgag ggagaagaac cagacacctg atctgccctc cgccgtcacg
      961 taggtgcgga ggttgatctc agggaagtcc gagagacgtg ggacggcggg aaggaagcgt
     1021 ggcctcacgc cggtcatgcg gaaaggaacg acgccgagcc aggcgcttcc ttcgaaggtg
     1081 tcgaggctca gggccggcgg tacgaggggc ctgagcgcat cctcttcgac gggccagtgc
     1141 atgaagagca ggttccgcca gctcatcccc atggcccacg agccggcatg aacggggtag
     1201 ggtcgatgct ccaccctgct cagaaccgac tctggtctaa aggcagcccg cataagcgaa
     1261 ggttaagacg tctgcgcctg ggagtccgta tgccctgtca cggtcacgtg gtctcatgca
     1321 ctacgaggag cgcaccgtcc ctcacgctca cccttgcctc cctgccggtc aactcgttgt
     1381 ggaaccccag agtgtcggcg ggctcgagaa ccgcatcttc gagtggatag gccatgcctt
     1441 ccagcgtgac gcccgtggcc gtcccggata gagggaagat agagatgctg tcacgtggcg
     1501 ctccctgaac cgcccgggat cctgcctcga ccacgaacat ccggccccag tcggccacga
     1561 ctgtcgcaag gagcccgatc cgccccgcta cgagcaggat gtgcgggacg gcgaagatgg
     1621 ccccgagctt tccccctacg gcgcagagga agtctacctc tgtagcacct ctctccctgg
     1681 ccgcaatgac cgcgagatga ccgtccatct tgtctttttg cacaggatgg cgttccagtg
     1741 aggctccgcg ttcttcgatc tcccgcgcga ggtcttctcc cagagagtcc atatccccga
     1801 cgaccaggtc ggggaagatc ccggcgtcca gcgcatagcg ggcaccgccg tcggcggcga
     1861 gtatcaggtc cgcccgcttt gcgacggccc ggatcagacc ccgcgaatcg ggatcgccgt
     1921 tcaaaaatac cgcggcccgc accttacgag gtcgccgagt gccggtagcg cagttgcctg
     1981 ggcgaaggct gcgccccttc tagcgccttc agcagcacca cgacgacgac gagcgcgatg
     2041 ccaaggctcg gggccaggta cgccgcgttg tacgccgccg agtagagata gacattccag
     2101 ccctccggcg cgtaggatgc gaagaagacg acgcccgaga ggaagtggca cacgaagcgc
     2161 gcaagcacgg ccaccgtggt cccaaggata gcgccgcgca ccgttggcct gaagaatcca
     2221 gcgagcccga gggcaccgaa ggcaagcgga taatccagga ggacctgcaa gggatgtacc
     2281 aggaaaggat caatggcgag gtcgaggaag ccataggcta ctcctgcaac gatccccacc
     2341 ttccaaccct gtctgaggga gagcaggatc aggggtacca tctccaggga gacagttccg
     2401 ccgaagggca tcttgaagag tacgacgagc cccagaacga aagccagcgc cacggcgatc
     2461 gccgcctcgg tcagcaccct tgtgtctctg aacgcgttca aaagcgcacc ctccttccaa
     2521 tatgcgggga tgcgccgaaa gattccgaaa cctccctgcg ccggcattac ccggatcagg
     2581 ttcagcgggt cggcacctgc gtgccctctc agcctatcgg ctccccggtc ttttattgtt
     2641 cccagtgtaa cgtactgcgg gtatccacac caccgcaggc actccacaat aacccgagca
     2701 cggtggcggc gtcggtgatg gaaccttccc tgatcccctc tatagcctcc tgaaaaggca
     2761 actccaccac ctgtatgaac tccgtgggct ctggccgcgg gccgcgctcg tccctcgccg
     2821 ccgcgcaccg gaacaggtgg cagacctccg tgctccgtcc cgtgctcgtg tgtaccgacg
     2881 tgagatgctc cagtccctcc gcccggtaac ccgtctcctc gaagagctcc ctacgggccg
     2941 cctgctcggg ctcctcaccg tcatcgaccg cgccgctcgg caactccagc gtgaaggcgc
     3001 caagtggctg tctccactgg cgcaccagta ccacgtct
//
LOCUS       DATAIR010000593         6597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_64449, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000593 DATAIR010000000
VERSION     DATAIR010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64449"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VHM69_14050"
     CDS             <1..760
                     /locus_tag="VHM69_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX2741564.1"
                     /translation="QCLSLKNGRVGIGTYDPQGTLDVRGDIRAGNSDIYFTQANHNHT
                     GIGNTAGYAALENAANYGALMILGRSGTPRGRYVRLWDYLQVNGGMDVTGSVGVGTDG
                     PQVKLHAVGNRIRIDNGGGRSLDLRADGSALDLESNGADLYINNNNRPVRIRNQQSVS
                     SRDLKENIADFTTREATEALEGLIPVSFNWNDDGEKTRHIGFIAEDAPEIAKSADEKA
                     IIPVHILAILSKVVQEQQRAIGAMRRQLDLREAT"
     gene            765..881
                     /locus_tag="VHM69_14055"
                     /pseudo
     CDS             765..881
                     /locus_tag="VHM69_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            990..2528
                     /locus_tag="VHM69_14060"
     CDS             990..2528
                     /locus_tag="VHM69_14060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2741565.1"
                     /translation="MTHGYMLSGTGSNVYVQNLCRALSREGHEVHLLCQEPDPLRYDF
                     VGEHSTVYGGRIEKLEERETPYAGRCAVYNPEIGDLLPVYVYDDYPGWRVKTFLDLTD
                     DELDNYLRRNIEAVRSVLGASGAEAVITNHSVPGPLISRRALEDTGVPYASIVHGSCL
                     QYVSRKSEKYMGLTREGLEAAREILALSSHSAGTIAEDFPDLAGKTRSLPGGVDTELF
                     HPDAFDRGVIENLNGGPGRGKEQRASLLEALEDSGNAVELAGALRAVASSYDARSHDG
                     DAGERLKSFLDREGPLVIYVGKLIHSKGVHSLISAFSRIRGETGARLLVIGFGTFREG
                     LEALTQSLSTGDETTLERLAEVGRLLEGGPAAPLEHFELTDELLDDARGIEDDILFTG
                     PLYHDELAKLLPAADAGVVPSIFPETFGLVAAELAASGVVPFVADHSGLREAGEIVGR
                     GLPFDLRIGMESFEENLARALADYLTLPEDQRRRCQGIVRHNSVEHLGWATLAGDLVV
                     LVQE"
     gene            2532..3767
                     /locus_tag="VHM69_14065"
     CDS             2532..3767
                     /locus_tag="VHM69_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2741566.1"
                     /translation="MEIEHILRDFGLILGAGLVSQLIATLIRLPEMVVLVAAGALIGP
                     SVLGLVSNPLGGVGAQLLFNIGVALILFHGGTGISLRVISKTAVGLGLLVLPGVLLTA
                     FIVALVVSPVFGVAFPVALLVGAVLASTDPAILIPLFERLKLRPKVSQTVIAESAFND
                     VTGTVLTLTLVGAVEAGRITFSGPALEFTKELALGGLIGVAAGAVLCYAVASTARAGI
                     WDESPGVAILAVVALAYFSTESVGGSAYLATFVMGLMIGNMEFLGLGHHDEHAALLEG
                     FLGQVAEIATLLVFVTLGLNLPFEALGKYFFGGLLVMFVFIFVARPITVLACLLPDRR
                     GEWTGNEIAFISWCRETGVIPAAVASLLLARGVEGAEIAVSMVALAVCVTLLLQATTA
                     GYVARRLGLLEGQEEESPA"
     gene            3980..4966
                     /gene="moaA"
                     /locus_tag="VHM69_14070"
     CDS             3980..4966
                     /gene="moaA"
                     /locus_tag="VHM69_14070"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX2741567.1"
                     /translation="MAQLTDSYNRKMDYLRISVTDRCNFRCQYCMPEDIKFQDKSHIL
                     TLEEMLTFAEACLQLGVTKVRVTGGEPLVRRGVVMFVGWLKDLGFEDVTMTTNGFLLK
                     ENLDGLVEAGLDRINISLDTLQPEKFAFITRRNHFQKVWDAVMAALETPLSPVKINAV
                     AMRGVNDDEISDMAKLTLEYPMHVRFIELMPLNGDTDGSRFRSLFISGREILDRAQDE
                     LGDLRPIEKEDPAAPADEFKFDGAPGTFGVITPVSEPFCARCSRLRLTADGKIHPCLL
                     TDLDVPVKHAIRSEDPIPAIHKVLWETARIKPAAGLTLPEESRHRTMSQIGG"
     gene            complement(4995..5240)
                     /locus_tag="VHM69_14075"
                     /pseudo
     CDS             complement(4995..5240)
                     /locus_tag="VHM69_14075"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5258..5467)
                     /locus_tag="VHM69_14080"
     CDS             complement(5258..5467)
                     /locus_tag="VHM69_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741568.1"
                     /translation="MANVDPDKDALKQALKEALTETLHEQREWLHEVFAEVVEEIGLA
                     EAIREGRRTDLAERGEVFDVLEDRT"
     gene            complement(5794..6123)
                     /locus_tag="VHM69_14085"
     CDS             complement(5794..6123)
                     /locus_tag="VHM69_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX2741569.1"
                     /translation="MQTIPPLHGVAIRSTSSTETKPGIEIERSVTEEPPYKVILHNDD
                     YTPMEHVVATLQKVIPRMSVRRAVDIMMEAHTKGKAVATKCHKELAELYQEGLNNEGL
                     IASIEPD"
     gene            complement(6202..>6597)
                     /locus_tag="VHM69_14090"
     CDS             complement(6202..>6597)
                     /locus_tag="VHM69_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX2741570.1"
                     /translation="QNGCDGHTLDPLTHLSATTRFYEHVPNRMHDLAHELCEGRWVAT
                     GGGGYDIWRVVPRAWTALWAAVSHQELPEKVSEDWLSKWGAKSPVKLPRLMRDDPEDY
                     PRGPRAANIASRNERTVEEVVENVIPMIR"
BASE COUNT         1236 a         2120 c         1967 g         1274 t
ORIGIN      
        1 ccagtgcctc tcgctcaaga acggcagagt cggcatcgga acctacgacc cacaggggac
       61 attggatgtc aggggagaca tccgcgccgg taactccgac atctacttca cccaggccaa
      121 ccacaaccac accggcatag gtaacacagc cggctatgcg gcgctcgaga acgcggcaaa
      181 ctatggcgcc ctgatgattc tcggtagatc gggaacgcca cgcggccggt atgtcaggtt
      241 gtgggactat ctacaggtca acggtgggat ggacgtgacc ggctctgtcg gcgtaggaac
      301 agatggccct caagtcaagc tgcatgcggt cggcaaccgc atccgcatag acaacggagg
      361 cgggcggtct ctagatctaa gggccgatgg ctcagccctg gatctggaat ccaacggtgc
      421 cgatctgtac atcaacaata acaaccgtcc ggttcgcatt cggaaccagc agtcggtctc
      481 atcccgagac ctgaaggaga atattgcgga ctttaccacc cgagaggcca cggaagccct
      541 ggagggcctg atccccgtct ccttcaactg gaacgacgac ggggagaaga cgaggcacat
      601 cggcttcata gccgaggacg ccccggagat agcgaaatcg gctgacgaaa aggcgatcat
      661 acccgtgcac atactggcca tactgagcaa agtggttcag gagcagcagc gggcgatcgg
      721 cgcaatgcgt cggcaactcg acctgcggga agctacataa cgatctcgta gcgggcgaaa
      781 acccgtagcg cccgcgcggc gaagccgtct ttgccggcct gtgcgtggtc tcacccgata
      841 ctgcgtcttc cggcaagagc ttacgccaac gtctaagacg aagcgcgaga tctcgcgccg
      901 tcaaaggcgc tgtgctgcct gagctgtgtc ttgaaccgta gtcatcaggc cctgtgcctc
      961 tgctatccta cttcgggatg aagatcttga tgacccacgg gtacatgctc tcgggcacgg
     1021 gcagcaacgt ctacgtccag aacctgtgcc gcgccctctc gcgcgagggc cacgaggtgc
     1081 atctgctctg ccaggagcct gatcccctgc gctacgactt cgtcggggaa cactcgaccg
     1141 tgtacggtgg caggatcgaa aagctggaag agcgggagac accatatgcc gggcgctgcg
     1201 ccgtctataa cccggagata ggggacctcc ttcccgtcta cgtctacgac gactaccctg
     1261 gatggcgcgt gaagaccttc ctggacctga ctgatgacga gctggacaat tacctcaggc
     1321 gcaacatcga ggctgtgcgg agcgtgctcg gcgcgtctgg cgctgaggct gtcatcacca
     1381 accattccgt gccgggcccc ctgatctcgc gccgcgcact cgaagacacc ggcgtaccgt
     1441 acgccagcat cgtgcacggg agctgcctgc aatacgtatc ccgcaagagc gagaaataca
     1501 tgggcctcac ccgcgagggc ctggaagcgg cgcgggagat ccttgccctc tcatcacaca
     1561 gcgcgggcac catagcggag gacttccccg acctcgccgg aaagacccgc tctctccctg
     1621 gcggcgtgga cacggagctg ttccaccccg acgcgttcga ccgcggggtc atcgaaaacc
     1681 tcaacggcgg ccccggtcgc ggcaaggagc agcgggcttc gctactagaa gccctggagg
     1741 attcgggcaa cgccgtagag ctggcaggcg ccctgcgcgc ggtcgcctcc tcctacgacg
     1801 cccgctcgca tgacggggac gccggtgagc ggttaaaaag cttcctcgac cgcgaggggc
     1861 cgctcgtgat ctacgtcgga aaactcatcc actccaaggg agtgcacagc ctcatctccg
     1921 ctttttcccg gatacggggg gagacaggtg cccgcctcct cgtgattggc ttcggcacct
     1981 tccgcgaagg actcgaggcc ctgacccagt cgctgagcac gggcgacgag acgaccctgg
     2041 agaggctcgc cgaggtgggc aggctgctcg aaggcggacc agcggccccg ctcgagcact
     2101 tcgagcttac cgatgagttg ctcgacgacg ccaggggaat cgaggacgac atactcttca
     2161 ccggccctct ctaccacgac gagttggcga agctcctccc cgccgctgac gcaggagtcg
     2221 tgccttccat cttccccgag accttcggcc tcgtggcggc cgagctcgcc gcgagcggcg
     2281 tcgtgccttt cgtcgctgac cactccggcc tcagggaggc cggagagatc gtgggccgcg
     2341 gtctgccctt cgacctccgc atcggcatgg aaagcttcga ggagaacctg gcaagggcgc
     2401 tggcagatta cctgacattg ccggaggacc agaggcgacg gtgccagggt atcgtgcgcc
     2461 acaacagcgt tgagcatctg ggttgggcga cgctggccgg ggacctcgta gtcctcgtcc
     2521 aagagtagag cttggagata gagcacatac tccgcgactt cggcctcata ctaggggccg
     2581 ggctcgtctc gcagctcata gcgaccctca taaggctccc cgagatggtc gtgctcgtgg
     2641 cggcgggcgc gctcataggg ccttccgttc tcgggctcgt ctcgaatcca ctgggcgggg
     2701 ttggagcgca actcctcttc aacatcgggg tggcgctcat cctcttccac ggcggcacgg
     2761 gaatctcact gcgtgtgatc tcgaagaccg cggtcggcct cggcctgctc gtcttgccgg
     2821 gcgtgctcct gacggccttc atcgtcgcgc tcgtagtctc gcccgtgttc ggcgtcgctt
     2881 tccccgtcgc cctgctcgtt ggagcggtcc tcgcttcaac ggaccccgcc atcctcatcc
     2941 ccctcttcga gcgcctgaag ctccgcccca aggtctccca gacggtcatc gccgagtcgg
     3001 cgttcaacga cgtgacgggc acggtactga cgctgaccct ggtcggcgcg gtggaggcgg
     3061 gccgtatcac cttctccggt cccgctctgg agttcaccaa agagcttgcg ctcgggggtt
     3121 tgatcggcgt cgcagcgggc gccgtgctct gctacgcggt cgcctcgacg gcgcgggcag
     3181 gaatatggga cgagtcgccg ggggtggcta tcctggctgt cgtggcgctt gcgtacttct
     3241 cgaccgagtc cgtcggcggg tcggcctacc tcgcgacctt cgtgatgggg ctcatgatcg
     3301 ggaacatgga gtttctaggg ctcggccatc acgacgagca cgccgccctc ctcgaagggt
     3361 tcctcggaca ggtagcggag atagccaccc ttctcgtctt cgtcactctc gggctaaatc
     3421 tgcccttcga agcgctcgga aaatacttct tcggcggact cctggtgatg ttcgtgttca
     3481 tcttcgtggc ccgtcccata accgttctcg cgtgcctcct gccagaccgg cgcggtgaat
     3541 ggaccgggaa tgagatcgcc tttatcagct ggtgccgcga gacgggtgtg atccctgcgg
     3601 ccgtcgcgag cctgctcctg gcgcgcgggg tcgagggggc ggagatcgcc gtctccatgg
     3661 ttgccctcgc tgtctgcgtg acgctgctcc tgcaagcaac gaccgcaggc tacgtcgccc
     3721 gccgtctcgg cctgctcgaa gggcaggaag aggaatctcc cgcctgaaac gtcgatctgc
     3781 acgccgggct tgcgtgctgc cgatgtcagc cgattcgagt tacattgcat agccctcacc
     3841 ctatttctat ctcctccgaa tctctaacgg taaacagcag ccgcaaactc tgggcagtat
     3901 gaaggtgggc atctctttgg cggctcctct ctccgaaagg agatacaatc tgcggagcag
     3961 ggttcggcga aaggatatct tggcgcagct gaccgacagc tacaaccgca agatggatta
     4021 cctcaggatc tcggtcacgg accgctgcaa cttccgctgc cagtactgca tgcctgagga
     4081 catcaagttc caggacaaga gccacatact cacgctcgaa gagatgctca ccttcgccga
     4141 agcgtgcctg caactcggcg tgaccaaggt ccgcgtgaca ggcggcgagc cgctcgtcag
     4201 gcgcggggtc gtcatgttcg tcggatggct caaggacctc ggcttcgagg acgtgaccat
     4261 gaccaccaac ggcttcctac tcaaggagaa cctggacggc ctggtcgagg ccggcctcga
     4321 caggatcaac atctccctcg atacactaca gcccgagaag ttcgccttca taacacgccg
     4381 caaccacttc cagaaagtct gggacgccgt gatggcggcg ctcgagacac ccctgtcgcc
     4441 ggtcaagatc aacgccgtgg cgatgcgcgg cgtcaacgac gatgagatct ccgacatggc
     4501 gaagctcacg ctcgaatatc ccatgcacgt caggttcata gagctcatgc ctttgaacgg
     4561 cgacaccgat ggatctcgct tccggtcgct cttcatctcg gggcgtgaga tcctcgaccg
     4621 agcccaggac gagctgggtg atctacgccc catagagaaa gaagaccccg ccgcccccgc
     4681 ggacgagttc aagttcgatg gcgcacctgg caccttcggc gtcattacgc cggtatcgga
     4741 gcctttttgc gccaggtgca gcaggctacg tctgacggct gacggcaaga tccacccctg
     4801 cctactgacc gacctcgacg tgcccgtaaa acacgccatc cgctcggaag acccgatccc
     4861 tgcgatccat aaggtcttgt gggagacggc gcggataaaa cccgccgccg gcctcaccct
     4921 cccggaagaa tcgcgccacc gcacgatgag ccagatcggg ggctaacaag cccacctcta
     4981 cgcgaggcgt cgacttatgg gaagtaccgg tggatgtcac gccggtgcag acagcggacg
     5041 aactcgacca cgtctccttc aatcgcgagg ccgatccgat agtcgccgac acgaactcgg
     5101 tagagacctc ccgtaccgct aatcttcttg aggttcggca cctcgttcag cgtcctggcc
     5161 gcttcgactc gttcgatgat ttcgcgtatc cggcgcaaca gggactggtc acgaatcttc
     5221 ttcaagtcgc gtacgaaact tctccggaac tcggtctcta cgtccgatcc tccagaacgt
     5281 cgaacacttc gccgcgctcc gcgagatcgg tcctccgtcc ttcgcggata gcttcggcca
     5341 ggccaatttc ttcgacgacc tcggcgaaga cctcgtgcag ccattcgcgc tgttcgtgca
     5401 gcgtctcggt aagcgcctcc ttcagcgcct gcttgagagc atccttgtcc ggatcgacgt
     5461 ttgccatctc gcctccgcga ttacgggagt ctggtcgaga gtatcgcaca cctagtcaca
     5521 gctgaaagac tttcttcacc cactcatctt gttccaaaaa tgggccgtgg atcgttccga
     5581 agtaatcgcg attcgatcac cacgagcaat ctgatcacat agcttagcca tctgcggctt
     5641 gtcggcatcc gaccaaccaa gtttccagta gccccggcga acgatcttcg gagtctggtc
     5701 gtatcgacca ctatacatag caccaaccac ccagtaattc ggcatttcac tccttcagca
     5761 gcgcggccgc cttaccattg tgctgagcta tgactaatcg ggttctatgg aggcgatgag
     5821 gccctcgttg tttagtccct cctggtagag ctcggcgagc tctttgtggc atttggtggc
     5881 gacggctttg cctttggtat gggcctccat catgatatca acggcgcgcc gcacggacat
     5941 gcgcgggatg actttctgca gcgtggcgac gacgtgctcc atcggagtgt agtcgtcgtt
     6001 gtgcaggatg accttgtatg gaggctcctc ggtgacgctg cgctcaatct ctatccccgg
     6061 cttcgtctcc gtggaagagg ttgagcggat cgccacgcca tgcaacggtg gtatggtttg
     6121 cactgtcatc tccggtccga agtgttgatc actcaaggca aaataccgcg acgccgccag
     6181 aatcgcaacc ctttttcttc tctaacggat catgggtatc acgttctcca cgacctcctc
     6241 gacggtacgc tcgttccgac tcgctatatt agcggcgcgc ggtccccgcg gataatcctc
     6301 gggatcgtcg cgcatgaggc gaggcaactt cacggggctc ttcgctcccc acttcgagag
     6361 ccagtcttcg gatacttttt caggtaattc ctggtgagag acggcggccc agagggccgt
     6421 ccaggcacgc ggtacgacgc gccagatgtc gtagccaccg ccgccagttg caacccagcg
     6481 gccctcgcag agctcgtggg cgagatcgtg catcctgttt gggacgtgct cgtagaagcg
     6541 ggtcgtagcc gagaggtgtg tcagggggtc gagcgtgtgg ccgtcgcaac cgttctg
//
LOCUS       DATAIR010000594         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_410177, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000594 DATAIR010000000
VERSION     DATAIR010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410177"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHM69_14095"
     CDS             complement(<1..346)
                     /locus_tag="VHM69_14095"
                     /inference="COORDINATES: protein motif:HMM:NF020856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX-like domain-containing protein"
                     /protein_id="HEX2741571.1"
                     /translation="MTDSEPALWFAFQGDRLLVFEDDPIRVPLASGLEHLGLETAFRW
                     EIGELDGHACWAAEIDSGTGPPEGMAFRDLRSLFYGVDEEFFRMAGRAKQIVGWHTTH
                     RFCGRCGGKTEPA"
     gene            complement(355..>2392)
                     /locus_tag="VHM69_14100"
     CDS             complement(355..>2392)
                     /locus_tag="VHM69_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2741572.1"
                     /translation="SFQFDSLAAASRATGRFLGMAPSAIELLDDVAIGRARANPAYRD
                     STRFVKGDPKAILVVEWSGTEVELDERFAKLDDLAGEVGALAVTPLRTKEEMSQTVRL
                     RKSTLPLLLGTTDKEKPVAFVEDAAVPPDRLEEFVVRFEEIVDRNGTWACFYGHASVG
                     CLHVRPALDTSDPEGVSRMRRIGEEVADLVVECGGSISGEHGDGLSRSEFLEKMYGKE
                     VLRAFAEVKRLFDPEGMMNPGVIVDPPPMDRQLRIGPGRKRLPINTNLSFRDQGGFAM
                     AVELCNGSGLCRKRTGGTMCPSYMVTLDEQDTTRARANMLRSVIEGTLPQEMTGRRMK
                     EVMDLCVGCKACKTECPSRVDVASMKTEVLYQTGKEHGFSMRQKAAGHIRRQLALAAL
                     VPSLYNAVAGTKLARRAAAFAGIDPRRSLPRVTKKPFSKRFPELPQGAGPAEVALFND
                     TWNEYQRPEVGEGAVRLFAAAGARVHLPEVVCCGRPMLSEGLVDEARKNARRNLDLLL
                     PLIERGVPLVGLEPSCILTMRDDYRKLLPDDERVEKLAEATRLFEEALLELDAELPLE
                     EGSPVLLHGHCHQKALVGTGPTERALALAPGSDVEVVDSGCCGMAGLFGYEKGHYEVS
                     MKMGERRLFPAVREAGERVVVAPGTSCREQILDGTERRALHPAEYLASLLDGST"
BASE COUNT          402 a          830 c          716 g          444 t
ORIGIN      
        1 gcgccggctc cgtctttccg ccgcagcgtc cgcagaaccg gtgcgtcgtg tgccagccga
       61 cgatctgctt cgcccgtccc gccatcctga agaactcttc gtccaccccg taaaagaggc
      121 tcctgagatc ccggaaagcc atgccctcgg gcggcccggt gccagagtct atctccgcgg
      181 cccaacaggc atgaccgtcg agctccccga tctcccatcg aaaagccgtc tccaggccga
      241 gatgctccag gccacttgcc agtggaaccc tgatagggtc gtcttcgaac acgagcagcc
      301 tgtcgccctg gaacgcgaac cacagcgccg gctccgagtc cgtcaatgtg gtttctatgt
      361 tgaaccgtcc aggagcgagg ccaggtactc ggcagggtgc agtgcccgcc gttctgtgcc
      421 gtccaggatc tgctcccggc aactcgtccc cggcgcgacc accacccgct cccccgcctc
      481 gcgcacggca gggaacaacc tcctctcccc catcttcatc gaaacctcgt agtgaccctt
      541 ctcgtacccg aagagcccgg ccatcccgca acatcccgag tccacgacct cgacgtcgga
      601 gcctggagcg agggccaatg ctctctccgt cggtcccgtc cctacgagcg ctttctggtg
      661 gcagtggccg tgcaggagga cgggtgagcc ttcttcgagc ggaagctccg cgtcgagctc
      721 gagcagcgcc tcctcgaaga gacgggttgc ttcagccagt ttctccacgc gctcatcatc
      781 tggaagcaac ttcctgtagt cgtcacgcat ggtgaggata cagctcggct ccagtccgac
      841 gagcggaacg ccgcgctcta tgaggggcag caggagatcc aggttccgcc tcgcgttctt
      901 cctcgcctcg tccacgagcc cctcggagag catcggcctt ccgcagcaga ctacctcggg
      961 cagatgtacc ctcgcgcccg ctgctgcgaa gaggcgcacg gcgccttccc cgacttccgg
     1021 cctttggtac tcgttccagg tatcgttgaa gagcgcgact tccgccggtc ctgcgccctg
     1081 cggcaattcc gggaagcgct tcgagaaggg cttcttggtc acacgcggca acgagcggcg
     1141 ggggtctata cctgcgaacg cggccgccct gcgcgcgagc ttcgttccgg cgacggcgtt
     1201 gtagagcgat gggacaagcg ccgctagcgc aagctgtctt cggatgtgtc ccgcagcctt
     1261 ctggcgcatg gagaagccat gctctttgcc cgtctggtac aggacctcgg tcttcatcga
     1321 ggcgacgtca acgcgggagg ggcattccgt cttgcaggcc ttgcagccga cgcagaggtc
     1381 catgacctcc ttcatgcgcc gtcccgtcat ctcctgcggg agggtgcctt cgatcacgga
     1441 ccggagcatg ttggcacgcg ccctcgttgt gtcctgttcg tcgagggtca ccatgtaaga
     1501 gggacacatc gtgccgcccg tcctcttgcg gcacaacccg ctcccgttgc aaagctcgac
     1561 ggccatggcg aagccgccct gatccctgaa tgagaggttc gtgtttatgg ggagacgctt
     1621 ccggccaggg ccgatacgca actggcggtc catcggaggt gggtccacta tgacgccggg
     1681 gttcatcatc ccttcggggt cgaagagacg cttgacctct gcgaaggcgc gcagtacctc
     1741 tttgccgtac atcttctcca ggaactccga gcgcgagagg ccgtcgccgt gctccccaga
     1801 gatggaacca ccgcattcca cgacgaggtc cgcgacctcc tcgccgatac gccgcatccg
     1861 tgagacacct tccgggtcgg aggtatccag cgccggcctg acgtgcagac accccacgct
     1921 ggcgtgcccg taaaagcagg cccaggtacc gttcctatct actatctcct cgaagcgcac
     1981 cacgaattct tccagccgat cgggaggaac cgctgcgtcc tcgacgaacg cgacgggctt
     2041 ctctttgtcc gtcgtaccga ggaggagcgg cagggtggac ttgcggagcc tgaccgtctg
     2101 gctcatctcc tccttcgtgc gcagcggcgt cacagccaga gccccgactt cacctgccag
     2161 atcgtccagc ttcgcgaagc gttcgtccag ctctacttcc gtgccgctcc actcgactac
     2221 caggatggcc ttgggatcgc ctttgacaaa acgggtcgag tccctgtagg cagggttggc
     2281 cctggcgcgg cctatcgcga cgtcgtcgag gagttctatc gcggatgggg ccatcccgag
     2341 gaaccggccc gtcgcgcgcg aggcggcagc cagggagtcg aactggaaag ag
//
LOCUS       DATAIR010000595        10216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_410196, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000595 DATAIR010000000
VERSION     DATAIR010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410196"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VHM69_14105"
     CDS             <1..254
                     /locus_tag="VHM69_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741573.1"
                     /translation="AIAALFQPLRRRLQNVIDRHFYRKKYDAARTLEAFSDRLRHKTD
                     LEHLNAELLSLVRETMQPAHVSLWLKPAGAGRSAEQRTG"
     gene            251..1501
                     /locus_tag="VHM69_14110"
     CDS             251..1501
                     /locus_tag="VHM69_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741574.1"
                     /translation="MSARESRRVGDQRSPLFARVVAWLVVLIYVAGTGANIWLQDRLD
                     VQGDPLLEDVLLRAGFGAFAVVGALLIAKRPTNLVGCIMAVIGIMVGIFPAGDSYAAY
                     VMTTRGHPDALAIVGAWAQGWYWYLLLALALIYLPLLFPDGRLPSRRWLPFALMMGTV
                     TLTLVILGALIDTLSSQDADYRIENPIGVESLAPVESLPVFGVFGVLLGVGLVGAIGA
                     VIVRFRRARGIERQQMKWFLYAAALIPSFLALDYLPGILSSVVLGLVILALPTAIGIA
                     VLRYRLYDIDLVVNRTLVYGSLTAALVLVYLGGVVSLQYVFRAFTGSESQFAVVVSTL
                     AIAGLFNPLRHRVQTFVDRRFYRRKYDAVKTLEAFGSRLRDDTDLDDLSGHLIEVVRE
                     TMQPAHVSLWMRTTEQGAGKRAGA"
     gene            1498..2781
                     /locus_tag="VHM69_14115"
     CDS             1498..2781
                     /locus_tag="VHM69_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741575.1"
                     /translation="MTARSAAPSRLSGRGLFLARTAWLVVALLAVAIFVAGAWLRFAE
                     LQQVCTASASACARQSLPTPENVRELHSLGLTLDFWALYSSATSLIFASVWWVVGAVI
                     FARRSEDRMALLVSIFLVTFTIAFWPDIPGALARAYPAFWTPVGIYRVLGDVLAMLFF
                     YLFPSGHFVPRWTRWLAVVWLVQRIFNIFTDSLSEPTFWLQLLVGLIFLGLLLSFIFA
                     QVYRYRRVSSPVERQQTKWVVFGVAVALVGIGTMLTPYFVFEPTSPSGTTTISVRTLI
                     QNTGGDAFMLLIPLSIGVAVLRSRLFDIDVLINRTLVYGALTVMLALVYVGSVVLLQG
                     ALRALAGGESQLAVVASTLTIAALFGPLRRRVQAFVDRRFYRRKYDAAKTLAAFSARL
                     REETDLDRLDKELVGVVKDTLQPKHVSLWLRDGGW"
     gene            2778..4055
                     /locus_tag="VHM69_14120"
     CDS             2778..4055
                     /locus_tag="VHM69_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741576.1"
                     /translation="MRSFWLAWSLAGLSVAMFLAGFALALLTMNVADPVMRPSVGGID
                     GLLIFLPFLAFPIVGALVASKRPRNPIGWLCLTAGLFWMLIVLGDQSTAYGLATTGSP
                     PGPVMLDALTLFAWVPPVGLLGAFMVMLFPDGRLPSRRWRPLAWFSGAAILLASVALE
                     LEPGRLSYRGGVRNPLGIEGHPWIQVVAVICILLLPLCIIASAFSLISRYRRSSREVR
                     QQIKWLAFAASFVGVTYLSALISGLFLAPDSLFTEGKSPAWVSLLFSAVLISYAGIPT
                     AIGIAILKYRLYDIDILINRTLVYGSLTATLAAFYFAGVAATQTTFRAITGQEQQPQL
                     AVVISTLAIAALFGPLRRRIQIVIDRRFYRRKYDAARILGTFSTRLREEVDLKSLRDD
                     LLEVVRETVQPAHVSLWLRPSEKVAPTSEEQDR"
     gene            4236..5420
                     /locus_tag="VHM69_14125"
     CDS             4236..5420
                     /locus_tag="VHM69_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741577.1"
                     /translation="MNRRTARSVALFLCALTLVLIACAVALSFQNRYPVENLYFLLTE
                     ASAAVVGGLIASRQPRNPVGWLIVGHALCFTLGEFGRQYAIYGVLTNPGSLPLARAMA
                     SPAYWAWFPGLLLMLAFLPLYFPNGRLLSPRWRPVLWFTVFSAVVITVLTMFRPSSDE
                     TRGIPNPLGIDSLKNAGLLEVVVPSLWSILAVMSAVSLVMRFRYSSGEERQQIKWVAY
                     ALVLLIVVTVSNQLIWQNLLPGWFDIFFPLLLEVLWISIAVAIFRYRLYDIDLVINRT
                     LVYGALTLFLALIYFGGVVLLQATLRALTGQESSLAVVASTLLIAALFNPLRHRIQSF
                     IDRRFYRRKYNAERVLATFSSRLRDETDLDRLSEELLVVVRETVQPAHASLWLRGARE
                     RT"
     gene            5455..6735
                     /locus_tag="VHM69_14130"
     CDS             5455..6735
                     /locus_tag="VHM69_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741578.1"
                     /translation="MVATGTPSPRWLHGRWLLAARATWLAVVALTIGLFVVSVPAGYS
                     LLRKVCTQKPCGPEQLSPEGAQSISQLGMSLGWYAAYNTALVVVFAIVFCAVAFVIFW
                     RRSDDIMALYTSLTLVLLGMFLPEWTAELLWPLYPSLRLTLELLTSLMFTCLFILLYI
                     FPDGRFVPRWTLWLAVVWVAIQVSSYVFPDLNFSPASWSSILRELVVAGLIFTCLFAQ
                     VYRYLKVSGPVERQQTKWVVFGLMATLVVLILVSVPPEFYPSLGQPGTLYDLILDFVS
                     FWIVLLVPLTFGIAILRHRLFDIDVIINRTLVYGTLTATLVVVYVVAVVAFQTVLHAL
                     TGSDSQLAVVASTLNIAALFNPLRRRIQSFIDRRFYRRKYNAARTLEAYAARLREETD
                     LEQLSGALVSVVEKSVQPAHVSLWLRAPESGERR"
     gene            6732..7949
                     /locus_tag="VHM69_14135"
     CDS             6732..7949
                     /locus_tag="VHM69_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741579.1"
                     /translation="MTRRAARRLAVATCALTLVLISGIGVMTFLNRGDIYDVNFTIVG
                     VSSAIVGGVVASRQVTNPVGWLFLGSALSAALRELAGAYAVYGIITNPGALPLPYAAA
                     WLSNAVVLIGPVMSFVLIPLYFPNGKPVSRRWSLVAWIALVSLPVITLLYALAPGEAV
                     NGSGIQNPLGVEALRPVMEGFQSIALVWYIGLILAAAASLVVRFRRSVGEERLQLKWF
                     TFAAAFIPVWFLVNSPIEAAIPNLFALLDSLIIAAVPIAAGIAILRYRLYDIDLIINR
                     TLVYGALTLFLALIYFGGVVVVQAALRAVSGQESSLAVVASTLMIAALFTPLRRRIQS
                     FIDRRFYRRKYDAARTLEEFGSHLREETDLDRLGEELVSVVRETVQPEHASLWLRPQQ
                     SSFDWREGGSRGV"
     gene            7942..9399
                     /locus_tag="VHM69_14140"
     CDS             7942..9399
                     /locus_tag="VHM69_14140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2741580.1"
                     /translation="MFEVVVIGGGPAGVTAALRARELGATVALVERANMGGTCTNDGC
                     VPTRVLARAARLMRDSEQFADYGLVGERPEVDFALLLNRTQRIVYEVQEKKQLRRHLE
                     AAGVIVLDRTGGAAFVDGHTLALGDGSRVSGERFVVCAGGHARRMDFPGSEHALTHSD
                     VWTMKNLPSSVVVIGAAATGCQLASVFEAFGSRVRLLEVAPRILGGEDEVVSSGVAEA
                     FSRRGIEILTGIGGVERIVRDGEVLLLSYGQGDETRILETEAVVLAVGWPANVEALNL
                     GAAGVETDRGYVLVDDALRTSAPHVFAAGDVTGRMMLVQSATYEGALAAENAALGGSR
                     DYRHAIVPHGGFTDPEYASVGLTEIQARDELGDEDCAVAIVPYADLDRGVIDGHPDGS
                     CKLIVSRSSRLILGAHIVGEQAVEVVQLVAAAMDSGMRVEQLAHLELAYPTFTAILGL
                     AARQLARELDLVAASPRWRASERPGATEWEHNDRG"
     gene            9536..9946
                     /locus_tag="VHM69_14145"
     CDS             9536..9946
                     /locus_tag="VHM69_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741581.1"
                     /translation="MKISVLLRWVGPAAIAGGVFMVLSDLSGLPITLPYLSQTSPTGY
                     DAVGSGLILFALVLLLVGMVGLYAKKAEPNGARVIEYGYPTENTQLVEVQSAPEEAGY
                     QQAEEAVETVDPRASRAGALVMGAGLLLFFLLRD"
     gene            9986..>10216
                     /locus_tag="VHM69_14150"
     CDS             9986..>10216
                     /locus_tag="VHM69_14150"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2741582.1"
                     /translation="MTHRSRKRLELPSEVEEVFREFRTCEMSTLARDGTPITWPTLPF
                     WKPDEGRFMITTSISLSQKVSNVRRNPRVSLLF"
BASE COUNT         1634 a         3198 c         3176 g         2208 t
ORIGIN      
        1 tggctatagc cgcgctcttc cagcctctgc ggaggcgtct ccagaatgtc atagacaggc
       61 atttctacag gaagaagtac gatgcggcga ggacgctcga agccttctcc gacaggctgc
      121 gccataagac agatctggag cacttgaacg ccgagttgct gtcgcttgtc agggagacga
      181 tgcagccggc ccatgtctcc ctttggctga aacccgccgg ggcgggccgg agcgcggagc
      241 agcggaccgg gtgagcgcca gggagagccg gagagtcgga gatcagcggt ccccgctctt
      301 cgcgcgggtg gtggcgtggc ttgtcgtcct catctacgtc gccggcacag gcgcgaacat
      361 ctggctccag gaccgcctcg atgtgcaagg cgatcctctg ttagaggacg ttttgctgcg
      421 cgctgggttc ggcgccttcg cggtagtcgg tgcactgttg atcgccaaga gacccaccaa
      481 cctggtcggc tgtatcatgg ccgtcatcgg gatcatggtg ggcatcttcc ccgcgggcga
      541 ctcatacgcc gcctacgtga tgaccacccg cgggcaccca gacgcgctgg ccatcgtcgg
      601 cgcgtgggcc cagggctggt actggtatct gcttctcgcg ctggccctga tctatctgcc
      661 actgcttttc cccgacgggc gtctgccttc ccggcgctgg ctacccttcg ccttgatgat
      721 gggaaccgta acgttgaccc tggtcatcct cggggcgctc atcgacaccc tgagcagcca
      781 ggatgccgat tataggatcg agaaccctat cggtgtcgag agtctggccc cggtagagtc
      841 cctgccggtg ttcggcgtat tcggagttct gctcggcgtc ggattggtag gggcgattgg
      901 ggccgtcatc gtgcgcttcc gcagggcgcg ggggatcgag cgtcagcaga tgaagtggtt
      961 cctgtacgcg gcagcgttga tcccgagctt cctggcgctg gattacctgc cggggatcct
     1021 ttcgagcgtg gtattgggtc tggtgatcct cgccctgccg actgcgatcg ggatcgcagt
     1081 gctgcgctac cgtctctacg atatcgatct ggttgtcaac cgcaccctgg tctacggctc
     1141 tctgacggcg gcgctggtgc ttgtgtactt gggcggcgtc gtctctttgc agtacgtctt
     1201 ccgcgcgttt accgggagcg agtcgcagtt tgcagtggta gtctcgacgc tcgccatcgc
     1261 gggtcttttc aacccgctgc gccacagggt gcagaccttc gtggaccgcc gcttctaccg
     1321 gcgtaagtac gatgcagtca aaacgctgga agctttcggc tcccgcctca gggacgatac
     1381 ggatctggac gatttgagtg gccatttgat agaggtggtc agggagacga tgcagcctgc
     1441 gcacgtctca ttgtggatgc gcactactga gcaaggagcc ggaaagagag cgggagcgtg
     1501 acggcgcggt ccgccgctcc ttcgaggctg agcgggcgcg ggctgttcct tgccaggacg
     1561 gcatggctcg tggtcgcttt gctcgcggtg gcgatcttcg tcgcgggcgc ctggctgaga
     1621 ttcgcggaac tgcagcaggt gtgcacggct tcagcgagcg cgtgcgccag gcaatctcta
     1681 cccacgccgg aaaacgtgcg ggagcttcat tctctcggcc tcacgctaga tttctgggcg
     1741 ctgtatagca gcgccacgag cctcatcttc gcgtccgtgt ggtgggtggt cggcgccgtc
     1801 atcttcgcgc gcaggtcgga ggaccggatg gcgctactgg tctctatttt cctggtgacc
     1861 ttcacgatcg ccttctggcc cgacatacct ggcgcgctgg ccagggcgta cccagcattc
     1921 tggacacctg tcggaattta cagggtcctt ggcgacgtac tggctatgct cttcttctac
     1981 ctcttcccaa gcggacactt cgtgccacgc tggacccgct ggctcgccgt ggtctggcta
     2041 gtgcagagga tattcaacat cttcaccgac tcactctccg aacccacctt ctggctgcag
     2101 ttactcgtcg gcctgatctt tctcggcctc cttctcagtt tcatcttcgc ccaggtttac
     2161 cgttaccggc gggtctcaag tccggtcgag cgccagcaga ccaagtgggt cgtcttcggc
     2221 gtagccgttg cgctcgtcgg gatcgggact atgctgacgc cctacttcgt cttcgagccc
     2281 acatctccat caggtacgac aacgatatcg gtccgtaccc tgatccagaa caccggcggc
     2341 gacgcgttca tgctgctgat tcctctcagt atcggagtcg ccgtcttacg ctcgcgtctc
     2401 ttcgacatag acgttctcat aaaccgtacc ctggtctacg gcgctctgac ggttatgctg
     2461 gcgcttgtat acgtcggcag tgtggtgttg ctgcagggcg cgctccgcgc gctcgctggt
     2521 ggggaatcac agcttgccgt ggtcgcttcg acgctcacca ttgcagccct gttcgggccc
     2581 cttagacgcc gggtgcaggc tttcgttgac cgccgctttt accgcaggaa atacgatgcg
     2641 gcaaagacgc ttgccgcctt ctcggcaagg ctgagggagg agacggatct tgaccggctg
     2701 gataaggaac tggtcggcgt cgttaaggat accctgcagc cgaaacacgt ctcgctctgg
     2761 ctacgggatg gcgggtggtg agatccttct ggctcgcctg gtcgctcgcc gggctctcgg
     2821 tggcgatgtt cctggccggt ttcgcgctgg cgctcctgac tatgaacgtc gccgaccccg
     2881 tcatgcggcc ttccgtgggc ggcatcgacg gactgttgat cttcttgccc ttcctggcgt
     2941 ttcccatcgt tggtgccctg gtggcttcga agcgaccgcg caatcctatt ggttggctct
     3001 gcctgaccgc tggcctcttc tggatgctca tagtactcgg tgaccagtca accgcttatg
     3061 gcctcgcaac gaccggctcg cctcccggcc ctgtgatgct cgacgcgctg acccttttcg
     3121 catgggtgcc accggtgggg ctgctcggcg ccttcatggt catgctcttt ccggacggga
     3181 ggctcccttc gaggaggtgg cgtccgctgg cctggttctc gggggctgcg atcttgctcg
     3241 cgagcgttgc cctggagcta gaaccgggac ggttgtcgta cagagggggc gtacgcaatc
     3301 ctctggggat cgaaggccat ccgtggatac aggtcgtggc ggtgatctgt atcctgctct
     3361 tgccactttg tatcatcgcg tcggccttta gcctgatttc acgttacagg cgttccagta
     3421 gagaggtgcg ccagcagatc aagtggctcg ccttcgccgc ttcgttcgtc ggggtgacgt
     3481 acctgagtgc cctgatcagc ggcctatttc tcgccccgga ttctctattc accgaaggaa
     3541 agtcgccggc ctgggtctcg ctgctcttta gtgctgtcct gatcagttac gccggcatcc
     3601 caacggccat cggcattgcc atcctcaagt accgtctcta cgacatagac atactcataa
     3661 accgcacgct cgtatacggt tcgcttaccg cgacgctggc tgcattttac tttgccggcg
     3721 tggcggcgac ccagacgaca ttccgcgcaa ttacaggcca ggaacagcag ccgcaactgg
     3781 ccgtagtcat ctcgaccctc gccatcgccg ctctgttcgg acccttgcgg aggcgcatcc
     3841 agatcgtcat agaccgacgc ttctaccgca ggaagtacga tgcggccagg atcctgggta
     3901 ccttcagcac ccgcttgcgt gaggaagtag acctgaagag tttgagggac gacttgctcg
     3961 aggtcgtgcg ggagaccgtg cagcctgccc atgtcagcct gtggctgcgg ccttcagaga
     4021 aggtggcgcc cacatcggag gagcaggaca ggtgaatatc cgctggatcg cgcgcccagc
     4081 gtgggtcctc tccccggcgc tcggcgtcct cagcttcccc tggacaatcg ttcagtcgaa
     4141 aatgaggtcg agcagacgat gcggcctgct catgcttcac tatggttacg tggaccgtag
     4201 gtacaggaga tgaacgtaag gatgcccaga gacagttgaa ccgaagaacc gcccgtagcg
     4261 tagctctgtt cctgtgcgcg ctgacgctgg tcttgatagc ctgtgccgtc gcactctcct
     4321 tccagaaccg ttaccccgtc gagaacctgt acttcctcct taccgaggcg tcagccgctg
     4381 tagtcggggg ccttatcgcc tcccgccagc ccagaaaccc tgtcggctgg ctcattgtcg
     4441 ggcatgcgct gtgcttcact ctgggcgagt tcgggcgcca gtacgctatc tacggcgttc
     4501 ttaccaaccc gggctccctg ccgctcgcca gggcgatggc ttcgccggcg tactgggcgt
     4561 ggttcccagg acttctattg atgctggctt tcctgcccct ctacttccct aacgggcgcc
     4621 tcctctcgcc acgctggcgc cccgtcctct ggttcaccgt cttcagcgcc gtggtcatca
     4681 ccgtcttgac catgttcagg ccgagcagtg acgagacccg cgggatcccc aatcctcttg
     4741 ggatagatag cctgaagaac gcagggttgc tggaggttgt cgtgccatcc ctctggtcga
     4801 tccttgccgt catgtccgcg gttagccttg tgatgcgttt tcgctactcc tcgggggagg
     4861 agcgccagca gatcaaatgg gtcgcctacg cgctggtgct tctgatcgtc gtgaccgtct
     4921 cgaaccagct catttggcag aatctccttc ccggctggtt cgacatcttc ttcccactac
     4981 tcctcgaggt actctggatc tctatcgccg tcgccatctt caggtatcgg ctctacgaca
     5041 tagacctggt cataaaccgc acccttgtct acggcgccct gacgctgttt ttggctctga
     5101 tctacttcgg aggcgtcgtg ctgctccagg cgacgctgcg cgcgctgacc ggacaggagt
     5161 cgtctctggc cgtggtcgct tccacgctgc tgatagcggc cctgttcaat ccactcagac
     5221 atcgcattca gtcgtttata gatagacgct tctaccgcag gaagtacaat gcggagcggg
     5281 tactggccac gttctcgagc aggctgcgcg acgagacgga cctcgacagg ctcagcgagg
     5341 aactgctcgt agtggtgcgg gagacggttc agccggcgca cgcgagcctg tggttacgcg
     5401 gtgctcgtga gaggacgtga acgggagggc atgagaacga gagaaggaga accgttggtt
     5461 gcgacaggca cgccgtcccc caggtggctg catggtcgtt ggctgctggc ggcgcgggcg
     5521 acgtggctcg cggtggtggc gctgacgatc ggcctgttcg tagtcagcgt ccctgccggc
     5581 tactcgcttc tgcgcaaggt atgtacccag aagccgtgcg gccccgaaca actctctcca
     5641 gagggggcgc agtcaataag ccagctcggc atgtcgctcg gctggtacgc cgcttacaac
     5701 acggcgcttg tggtcgtctt cgccatcgtg ttctgcgccg tagccttcgt tatcttctgg
     5761 cgaagatcag atgacatcat ggcgctttac acgtcgctca cgctggtgct gctcgggatg
     5821 ttcctgcctg aatggacggc ggagttgctg tggccgctct atccatccct gaggctgacg
     5881 ctcgaactcc ttacctcact gatgttcacc tgccttttca tcctgctcta catcttccct
     5941 gacggacgct tcgttccgcg ctggaccctc tggctggcgg tggtctgggt cgcgatccag
     6001 gtgtccagct acgtcttccc tgacctgaac ttctcccctg caagctggtc atctattctc
     6061 agggaattag tggtggcggg tttgatcttt acctgtttat tcgcgcaggt ctatcgttac
     6121 ctgaaggtct ctggacccgt ggaacgccag cagaccaagt gggtcgtctt cgggctgatg
     6181 gcgacgctgg tagtcctgat cctggtgagc gtgccgccag agttctaccc atcgctcgga
     6241 cagccaggga cactctacga cctcatactg gacttcgtct cgttctggat cgtgttgctt
     6301 gtgccgctga cgttcggcat cgcgatcctg cgccaccgcc tcttcgatat agacgtcatc
     6361 atcaaccgga cgctggtcta cggcacacta accgcgacgc tcgtcgtggt gtacgtggtc
     6421 gccgtagtcg cgtttcagac ggtcttacac gctcttacag gaagcgactc gcagctagcg
     6481 gtggttgcct cgacgctgaa catcgccgcc ctgttcaatc ccctgcgtcg ccgcatccag
     6541 tccttcatag acaggcgttt ctatcgtaga aagtacaacg cggccaggac gctcgaagct
     6601 tacgccgcga ggttgagaga agagacggac ctcgagcagt tgagcggcgc cctagtctcc
     6661 gtcgtggaga agtcggtcca gcccgcgcac gtctcgctgt ggctgcgggc gccggagagc
     6721 ggagagcgca ggtgacgcgc cgggcggcca ggcgtctcgc cgttgcaacg tgcgcgctca
     6781 ccctggtcct gatctccggt atcggggtca tgactttcct gaaccgtggc gacatctacg
     6841 acgtgaactt caccatcgtc ggggtgtcga gcgccatcgt cggcggtgtc gtcgcctctc
     6901 gccaggtcac caacccggta ggctggctct tcctcggcag cgcgctgagc gcggcgttga
     6961 gagaacttgc tggggcgtat gccgtctacg ggatcatcac caaccctggt gctctgccac
     7021 tgccgtatgc cgcggcgtgg ctgtcgaacg cggtcgttct gatagggcct gtcatgagct
     7081 ttgtcctcat accgttgtac ttccccaacg gcaagccagt atctcggcgc tggagcctcg
     7141 tcgcctggat cgccctcgtc agcctgcctg taatcaccct cctttacgcg ctcgctcccg
     7201 gcgaggcggt caacggctcg ggcatccaga acccgctggg ggtcgaagcc ttgcgacctg
     7261 ttatggaagg cttccagtcc atcgcgctcg tgtggtacat agggctgatc ctcgctgccg
     7321 cggccagcct ggtggtccgc ttccgtcgtt cggtgggcga ggagcgactg cagctcaagt
     7381 ggttcacctt cgccgccgcg ttcatccccg tctggttcct cgtcaactcc ccgatcgaag
     7441 ctgcgatccc caacctgttc gcgctgcttg actccctcat catcgcggcc gtcccgatcg
     7501 ccgctggcat cgcgatcctg cgctatcggc tctacgatat agacctgatc ataaaccgta
     7561 cccttgtcta cggcgccttg acgctgtttc tggctctgat ctacttcgga ggcgtcgttg
     7621 tggtgcaggc ggcgctcagg gctgtaagcg gtcaggagtc gtcgctggcc gtggtggcat
     7681 ccaccctgat gatcgcggcc cttttcactc ctctgcgtcg ccgcatccag tccttcatag
     7741 accggcgctt ctatcgccgc aaatacgacg ccgcgaggac gctcgaagag ttcggttccc
     7801 atctcaggga ggagacggat ctcgaccgtc tgggtgaaga gctggtctct gtggtcaggg
     7861 agacggtcca gccggagcat gcctcgctgt ggctgagacc gcagcaaagt agtttcgact
     7921 ggcgcgaagg cgggtcgagg ggtgtttgag gtcgtcgtta taggcggagg gccggccggg
     7981 gtgacggcgg ccctgcgcgc ccgtgagctc ggcgcaaccg tcgcgctggt cgaacgcgcc
     8041 aacatgggcg ggacctgcac gaacgacggc tgcgtcccta cccgcgtgct cgccagggcg
     8101 gcgcgcctga tgcgcgattc cgagcagttc gccgactacg gcctcgtcgg cgagcgaccg
     8161 gaggtggact tcgccctgct cttgaacagg acgcagcgga tcgtctacga ggtccaggag
     8221 aaaaagcaat tacgaagaca cctcgaagcg gctggagtca tcgtcctgga tcggaccggc
     8281 ggtgccgcct tcgttgacgg gcacacgctc gcgctcgggg acggctctcg cgtctcaggc
     8341 gagaggttcg tcgtctgcgc cggaggccac gcgcgaagga tggacttccc cggcagcgag
     8401 cacgccctga cccacagcga cgtgtggacg atgaagaacc tgccctcctc ggtcgtggtc
     8461 atcggcgcgg ctgctacggg gtgccagctt gcctcggtct tcgaggcgtt cggctccagg
     8521 gtaagattgc tcgaggtggc gcctcgcatc cttggcggcg aggatgaggt ggtctccagc
     8581 ggggttgcgg aggcgttctc ccgcaggggc atcgagatcc tcacaggcat cggcggggtg
     8641 gagcgcatag tgagagatgg cgaggtactt ctgctctctt acgggcaagg tgatgagacc
     8701 cgcattctcg aaacggaggc ggtcgtgctc gcggtcggct ggccggccaa cgtggaggcg
     8761 ctgaacctcg gcgccgctgg tgtggagact gatcgcggtt acgtgctcgt cgacgacgcc
     8821 ctgcggacga gcgccccgca cgtcttcgcc gccggagacg tgacagggcg gatgatgctt
     8881 gtccagagcg caacctacga gggtgccctc gccgccgaga acgccgcgct cggtggctcg
     8941 cgtgactaca ggcatgccat cgtcccgcac ggcggtttta cagaccccga gtacgccagc
     9001 gtgggcctaa ccgagatcca ggcccgcgac gagctcgggg acgaagattg cgccgttgct
     9061 atcgtgcctt acgccgacct ggaccgcggg gtgatagacg gacatcccga cgggtcctgc
     9121 aagctgatcg tatcccgctc ctcccgcctc atcctcgggg cgcacatcgt gggcgagcag
     9181 gccgtagagg tagtccagct cgtcgccgcg gcgatggact ccggcatgcg cgtggagcaa
     9241 ctcgcgcacc tggagctcgc ctatcccact ttcacggcga tactcggact cgccgccagg
     9301 caactcgccc gcgaacttga tctcgttgcc gcatcacccc ggtggcgtgc ctccgaacgc
     9361 ccgggcgcaa ccgagtggga acacaacgac cggggttgag ttcgtgcgcg tcagaaaaca
     9421 gaaaaaattg cgaaattctg caaaatactc tcgtcgtaac gcttctcgta acgacctcgt
     9481 aacgccaggc cactaggctg aaggcacaag agatcttacg agagaggaga ggcgaatgaa
     9541 gatatcggtc cttctgaggt gggtcggacc cgcggccata gcgggcggcg tgttcatggt
     9601 gctctccgac ctctctggtc tgcccataac ccttccctac ctgagccaga cttccccgac
     9661 gggctacgac gccgtcgggt cgggtttgat cctgttcgcg ctcgtgctgc ttctcgtcgg
     9721 catggtcggg ctctacgcaa agaaggccga gcccaacggc gccagggtca tagagtacgg
     9781 ctatcctact gaaaacacgc agctcgttga agtgcaatcc gcccccgaag aggccggcta
     9841 tcagcaggct gaggaagcgg tcgagacggt ggatccgcgg gcttctcggg ccggcgcgct
     9901 ggttatgggt gccgggctcc ttcttttctt cctgttgcgc gactagaata ggttggagag
     9961 cgtaaccggg gagaaggact gaaccttgac ccatagatcg cgcaagcgat tagagcttcc
    10021 gtccgaggtc gaggaggtct tcagggagtt ccgaacttgc gagatgtcca cgctagccag
    10081 ggacggcacc ccgatcacct ggcccacgct gcccttctgg aagcccgatg agggacgttt
    10141 catgataacg acctcgattt ccctctctca gaaggtgtcc aacgtccgcc gcaacccgcg
    10201 cgtctctctc ctcttc
//
LOCUS       DATAIR010000596         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_133562, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000596 DATAIR010000000
VERSION     DATAIR010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133562"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VHM69_14155"
     CDS             complement(<1..546)
                     /locus_tag="VHM69_14155"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX2741583.1"
                     /translation="MTTPQDNTKTTERAPRPSGSPPDEPRRTPGAGKQSAMPPRRTWL
                     WFALLLLANFLLVRFLLPGPEAPVEVPYTFFKEQVRAGNVESIYSQGDSVEGRFEEPV
                     TYPPKGQGGSEPSRTSKTFTTVLPSFVDPGLEDLLIDKGVEISARPIQEGGGLWQTLL
                     FAFGPALLLIGGYIWLYRRAQQ"
     gene            1325..1492
                     /locus_tag="VHM69_14160"
     CDS             1325..1492
                     /locus_tag="VHM69_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741584.1"
                     /translation="MDEARRDKERVASTKRGDRPLVELNRYVASHDVADFGTRVAPGG
                     DVPGDFGEELD"
     gene            complement(1993..3141)
                     /locus_tag="VHM69_14165"
     CDS             complement(1993..3141)
                     /locus_tag="VHM69_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2741585.1"
                     /translation="MGGSFKIGRFSGIDVRVHWTFFLLLAFFAFIGYQSSGSLAGALT
                     PTGVIVALFICVVLHEFGHSLVAQRLGIGIHSITLLPIGGVSNLESLPEKPADEVKIT
                     LAGPLVNVVLAPIFFGVGLLLGAEPRMPTDLFTGIGSVGQFFFYLGYLNVVLAVFNLI
                     PAFPLDGGRILRGVLATRLGALRATEIASRVGQLFALAFFIIGLLGGNILLALVAVFI
                     FFGASGEAQMVRQRELTHGLLVSDVMGTRSQTKTVSPYHTFVQVLDMVIHGYQEDFPV
                     VDDGKLVGMITRQEIMSAAHSPERYSTVRDLMKTNVPTIPSQSDLFKDGLRILQQSGL
                     RALPVTANGELVGMLTIEDIGQANLLRSLPGEMNRTGGRATSQQTASR"
     gene            complement(3189..4211)
                     /locus_tag="VHM69_14170"
     CDS             complement(3189..4211)
                     /locus_tag="VHM69_14170"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2741586.1"
                     /translation="MSFFRSLFPAHREAGTEILPSEGRLPPFDGATGWLNSEPLTPEG
                     LRGRVVAVQFWTYTCVNWLRTLPYTREWATKYQDQGLTVIGVHTPEFGFERNVDNVVA
                     AARDMRVDYPIALDSDYAVWRAFANHFWPALYIADGRGRIRYHHFGEGEYAMSEMVVQ
                     QLLREAGMEGFDPALVSVDPQGTEAPADWSNLRTPETYLSYGRAYGFASADGERFDEP
                     HDYPEPSRLSLNQWAPSGVWTLAEHAAVLNEAPGRIAIRFHARDVNLVMGPAERGLSV
                     QFRVLIDGQPPGDSHGTDVDDQGNGTVSEQRLYQLVRQPKPIADRQLEIEYLDSGVEV
                     YCFTFG"
     gene            complement(4208..4345)
                     /locus_tag="VHM69_14175"
     CDS             complement(4208..4345)
                     /locus_tag="VHM69_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741587.1"
                     /translation="MSSTSTRLPKPEGPGDWLAEQAAEEMLTALKDFLAPYREGGGGL
                     R"
     gene            complement(4411..4755)
                     /locus_tag="VHM69_14180"
     CDS             complement(4411..4755)
                     /locus_tag="VHM69_14180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2741588.1"
                     /translation="MSSTEQRASGIGRTDLQRHDLGIPGREVVHNRVDISPEAPAFEH
                     KLPGEEIIYVLEGSLAYQAEGKPPTTVEAGEVFFVPAETIHAVKNVGTGNAAELAKYV
                     IEKGKPLVVLAE"
     gene            complement(4837..>5620)
                     /locus_tag="VHM69_14185"
     CDS             complement(4837..>5620)
                     /locus_tag="VHM69_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741589.1"
                     /translation="LPSLPGYGFSSEPAELGWDAARTGRAWAQLMRRLGYTRYVAQGG
                     DVGALVTDLMGRQAVEGLVGYHLNLLTAVLAIGEHLPKESEQEQAAAAAVATFREDGF
                     AYFLEMATRPQTIGYALLDSPVALAAWLLDHDTDSYYKISRAFVDGEPVGNLTRETIV
                     DNITLYWLTGTGASAARSYWEDARALAASLASGQPPPPVTVPVGFTTFPDEIWASPRS
                     WVEAVYPGLAYFNEVDKGGHFAAWEEPQLFSEEVRAAFASLR"
BASE COUNT         1030 a         1807 c         1727 g         1056 t
ORIGIN      
        1 ctgctgcgcg cgcctataga gccagatgta gcccccgatc aggagcaaag ccggaccgaa
       61 ggcgaagagg agcgtctgcc acaagcctcc gccctcctga atcggcctgg cgctgatctc
      121 gacccccttg tctatcaaga ggtcttctaa gccgggatcc acgaaggacg gaagcacggt
      181 ggtgaaggtc ttggaggtac gcgaaggctc cgagcctccc tgacctttgg gcgggtaggt
      241 gaccggctcc tcgaagcggc cctcgacgga gtccccctgg ctgtagatcg actcgacgtt
      301 ccccgccctg acctgctcct tgaagaaggt gtagggcacc tcgactggag cttcggggcc
      361 gggcaagagg aaccttacaa ggaggaagtt cgcgagtagt aagagcgcga accatagcca
      421 cgtccttctg ggcggcatgg cgctctgctt ccccgctccg ggcgtgcgcc gcggctcgtc
      481 aggagggctc ccggaaggac ggggggctct ctcggtcgtc ttggtgttgt cttggggcgt
      541 ggtcatcagg tacctccgtc ggcgtgctga cgccttggtc cacttccaca aatatactca
      601 gccgcccagg aactgaccat gggtgctgat aaggtggcga accattgcgg gcagtgtacg
      661 cgggtcgccc acgtagtggc tgcggtctaa gaggttagtc tcgcggacct tctggcgggt
      721 gagatcggtg gtcacggcga ccatctccgt cccggccgcc agcgccggcc cccgatgccc
      781 cgcgatatat tcacatcgct catgttccat acctcctcgc tctttcgctt gcgatctggc
      841 ccctgtgaaa gaccgtatta gcaaacgccg tagatgccaa gaccaaaacc aacacaatca
      901 caataacgac gcgtttcatt actacttacc tcccatgtct tgttaccaca atgacaatat
      961 agagccgccc cctcttcttc ggagagacat ctgttctcct tcggcgagcg gagggacggc
     1021 gaggtgccac atcgcgagca tctgggggct gccgcggagc aacagcagaa ccggcggtcc
     1081 ctcaccactg cgaacggcgt tgatgctcgc tccttccgtc tcgaccgctc gaagccgcca
     1141 aacaaactgt atcttcccta gagcgcgttg tatgtcttcc gaccgcatac cagaggaatg
     1201 gcgtggtcca cccggagtca cggaaccttg ccttcaactg aagccggtcg cgaagggttg
     1261 cacctcgtat accacatagg tggaaaacat ctttcgtggt gacgcttccg atgtttctcg
     1321 cgttgtggat gaggccaggc gggataagga acgggtcgcc tctacgaagc gtggggatcg
     1381 ccccctcgtc gaactcaatc gctacgtcgc ctcgcacgat gtagccgact tcgggacccg
     1441 ggtggcaccc ggaggcgacg tccccgggga tttcggcgag gaactggact gactccgacc
     1501 ctgcgatcgg cgagggtagg ggctgcagct ccgttcgccc gaactccacg gtcgcggctg
     1561 accgggtggg ctccggctcg ctcatcatct gcggttctcc ctatcttgtg aacggcgact
     1621 gcttcttggg aaacgggtgg tgagcccgcc gactttctcg ccctcgtccg gggccgacga
     1681 cggcgatcac cgactgctgg ccgtttcgga acgtttcgct cgaaaggaag cgcgtcgtgc
     1741 cgaagccccg cagatacggg acgatcgccc tatagcccgc ctcggccagc aaggcgcaac
     1801 gtcgacatag ctgtgaatgt catagggcca gccgtgcaga aggatgaccg caggaacatc
     1861 ggaagggccg gcttcagcgt atccgacgtt caggacgccg gcgtcgatct gcttaatact
     1921 gttcatttgt tctccttcgc tgcccgcatg acgccgaagg ccgcggcgcc gatgacctct
     1981 atcccgcttc cgctaccggc tcgcagtctg ctggctggtc gctctgccac ctgtcctgtt
     2041 catctccccc ggtagcgacc tgagcaggtt ggcctggcca atatcctcga tggttagcat
     2101 tccgactagt tccccgttcg ccgtgacagg caacgccctg agcccgctct gctgcaggat
     2161 gcgaagaccg tccttgaaaa ggtcagactg ggaagggatc gtgggtacat tcgtcttcat
     2221 caggtcacgg acggtcgagt acctctctgg tgagtgggct gctgacatga tctcttgacg
     2281 cgtgatcatg cccacgagct tcccgtcgtc aaccacgggg aagtcctcct ggtagccgtg
     2341 gatgaccatg tccagaacct gcacgaaggt gtggtaaggc gaaacggtct tggtctgaga
     2401 cctggtgccc atcacgtcag agacgagaag gccgtgcgtc aactcccgtt gcctcaccat
     2461 ctgtgcttcg ccacttgccc cgaagaagat gaagaccgcc acgagggcga gcaggatgtt
     2521 gccgccgagc aaccctatga tgaagaaggc cagggcgaag agctggccta ccctcgaagc
     2581 gatctctgtt gccctgagcg ctcccaggcg ggttgcaagc actccacgca agatcctccc
     2641 accgtccaac gggaaggctg ggatcaggtt gaagaccgcc agaactacat tcaggtagcc
     2701 cagatagaag aagaactgcc cgaccgaccc gatgcctgtg aacaggtcgg ttggcatgcg
     2761 aggttcggct cccaacagga ggccaacgcc gaagaagatc ggggcgagca ccacgttcac
     2821 caagggaccg gcgagggtta tcttgacctc atctgcaggc ttctcgggga gggactccag
     2881 attagagacc ccgcctattg gcagaagggt tatgctgtgg atcccgatgc caaggcgctg
     2941 ggctacgagg gagtgtccga actcgtgcag caccacgcag atgaacagcg ccactatcac
     3001 gcccgtcggt gtgagcgccc cggccagact accagaactc tggtagccga tgaaggcgaa
     3061 gaaggccagg agaaggaaga aggtccagtg caccctcacg tctatgcccg agaatcgtcc
     3121 tatcttgaaa gaaccgccca tgaggtctct ccagttcgta gtagggattg aatagcctat
     3181 gctgtggatc atccgaacgt gaagcaataa acctccacgc ccgaatcgag atactcgatc
     3241 tctaactgtc gatcggcgat gggcttgggt tgccggacca gctggtacag ccgctgttcg
     3301 ctaaccgtgc cgttgccctg gtcgtcgacg tcggttccgt gagaatcgcc cggcggctgt
     3361 ccgtcgatga gcacgcgaaa ctgcaccgat agacctctct cggccggtcc catcaccaga
     3421 ttcacgtcgc gcgcgtggaa ccggatcgca atccgtcctg gcgcctcgtt gagcaccgcc
     3481 gcgtgctcgg caagcgtcca gacgcctgac ggggcccact ggttcaggct cagccgcgat
     3541 ggctcgggat agtcgtgagg ctcgtcgaac cgctcaccgt ctgcagacgc gaagccgtaa
     3601 gcgcgcccat agctcaggta tgtctcgggc gttctcagat tgctccaatc cgccggcgct
     3661 tcggtgccct gggggtcgac ggacacgagc gccggatcga agccttccat gcctgcttcg
     3721 cgcagcagct gctgaacaac catctccgac attgcgtact caccctcgcc gaagtggtgg
     3781 tagcggatcc gtccccgccc atccgcgatg tacagcgccg gccagaaatg attggcgaag
     3841 gcgcgccaga cggcgtagtc gctatcgagc gcgatcggat aatccaccct catgtccctg
     3901 gcggccgcca ccacgttgtc gacgttgcgc tcgaacccga actcgggcgt atgaaccccg
     3961 atcacggtca ggccctgatc ctggtatttc gtggcccact cacgcgtgta cggcagcgtt
     4021 cgcagccagt tgacgcacgt gtacgtccag aactgcaccg cgacgacgcg tccgcggagc
     4081 ccctcgggtg tcagcggctc ggagttgagc catcctgtcg cgccatcgaa aggtggcagg
     4141 cggccctcgc tcggcaagat ttccgttccg gcctcccgat gcgcggggaa gaggctccgg
     4201 aagaaactca tcgcaagcca cctccgccct cgcggtacgg ggccaggaag tccttcagcg
     4261 cagtaagcat ctcctcggca gcttgctcgg cgagccagtc gccgggacct tccggtttag
     4321 gtagccgggt cgaagttgag ctcatgacga tctcctttca cacggtcatc tagcgaagcg
     4381 acctgaatgc ggctcggacc tcgttttgtg tcactcggcc agcacgacga gcggcttccc
     4441 tttttcgatg acgtacttgg cgagctccgc cgcgttgccg gtgcctacgt tcttcaccgc
     4501 gtggatcgtt tcggccggga cgaaaaagac ctcgcccgcc tcgaccgtcg tcggtggctt
     4561 gccctcggcc tgatacgcca gcgacccttc gaggacgtag atgatctctt cgcccggaag
     4621 cttgtgctcg aaggctggcg cttccgggct aatgtccacg cgattgtgga cgacttcgcg
     4681 tccgggaatg ccgagatcgt gccgctggag atcggtgcgc cctatccccg acgctcgttg
     4741 ctccgtgctg ctcaatctca ccctccgttg tctcgtagtc gcccgacgca ggtcgcgggc
     4801 ggcgcacaga tcgtacgcgc cgcccacgtc cgccggttaa cgcagtgacg cgaatgcggc
     4861 tcgcacctct tctgagaaga gttgcggctc ctcccacgcg gcgaagtggc cgcccttgtc
     4921 gacctcgttg aagtaggcga ggcccgggta gaccgcctcg acccagctcc gaggcgacgc
     4981 ccagatctcg tcagggaacg tggtgaagcc gaccggcacg gtgaccggcg gaggaggctg
     5041 gccgctcgca agcgacgcgg ccagcgctcg tgcgtcctcc cagtacgacc gggccgccga
     5101 ggcgccggtg cccgtcagcc agtacagcgt gatgttgtcg acgatcgtct cccgggtgag
     5161 gttgcccacg ggctcgccgt cgacgaaggc gcgggagatc ttgtagtagc tgtccgtgtc
     5221 gtgatcgagc agccaggccg ccagcgcgac gggcgaatcc agcagggcgt agccgatcgt
     5281 ctgtggccgc gttgccatct ccagaaagta ggcgaagcca tcctccctga acgtcgcgac
     5341 cgccgcggcc gctgcctgtt cttgttcaga ttccttgggc aggtgctcgc cgattgcgag
     5401 cactgccgtg agcaggttca gatggtagcc gaccaaaccc tcgaccgcct ggcgacccat
     5461 caggtcagtg acgagggcac ccacgtcgcc gccctgggcg acgtagcggg tgtagccgag
     5521 gcgcctcatc agctgtgccc acgcgcgtcc ggtgcgggcg gcgtcccaac cgagctcggc
     5581 cggctcactg gagaatccgt aaccgggcaa tgacggcagc
//
LOCUS       DATAIR010000597        10782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_249263, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000597 DATAIR010000000
VERSION     DATAIR010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249263"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VHM69_14190"
     CDS             <1..1867
                     /locus_tag="VHM69_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2741590.1"
                     /translation="VGELETYESARLFAERARQRNPSFVLTASNAEAVSRVCRQLEGM
                     PLAIELAAARMEALSAEQLASRLHAPLKVLSRGERTAEPRHRSLRATLEWSHELLGEH
                     EQALFRRLSVFAGGWTLEAAEEVCSGDGIEEGDVLDLLSELVVRSLVVTEAGGEEALR
                     YGMLDLVRQYAREELEESGESESLRSRHAWYYLALAEREDDQDADPGVRRSRPVAWLR
                     QMEAEHANLRAALSWSLDGDAGQPEGRRVELGQRLATALFWFWQTHDYQTEGRSYLER
                     ALSNSTGTTTARLRAQALDAAGGIAGFQGDNGAAKALLQEGLALYRQLEDEEGIASTL
                     TDLGMIAVGGQREDIPLSAVMEELGELKPSLKSRNTLAWLHLVEGGIAVGRGDLEDSV
                     AHCERSLELFREIRDPRGILSCLLHLGMLALIGGNHEEAVPMLRESLRLAWDLDYTVV
                     IQCSLYSLACVAASREQPVLAARLWGTVEGLEEAYGVRIASLILSVTDYEGRLGIVRS
                     QLGEEACLEAWAEGKAMALERAVEYPLSEEEQQESPVRLAASERQPPPVDGRAERLTP
                     REQEIAILVARGFTNRQIASELFISERTVDHHVSNILKKLDVSSREQVASRLGDR"
     gene            2146..2337
                     /locus_tag="VHM69_14195"
     CDS             2146..2337
                     /locus_tag="VHM69_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741591.1"
                     /translation="MNHYLGLDPYVIKERNEEIRREVRALRLEGRLRDGHRQTPSRLV
                     LFALRSTLPLLRSVGLAER"
     gene            2567..2761
                     /locus_tag="VHM69_14200"
     CDS             2567..2761
                     /locus_tag="VHM69_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741592.1"
                     /translation="MTTGFIDAARDEGSFATLEGGGRAWLRLLASQEARHLREKEEAA
                     IEAEDYWEQAEEACAIDPIG"
     gene            2993..3475
                     /locus_tag="VHM69_14205"
     CDS             2993..3475
                     /locus_tag="VHM69_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HEX2741593.1"
                     /translation="MDIGVQPYALARENGQAVWFLGTLVIVKATGEQTGGAFGLIDNL
                     MPAGFASPYHMHRNEDESFYVVEGEMTFYVGDERVKAEPGAFVYGPRGVPHGFEVNGT
                     APARILLQNYPAGFEGFPVEVGEPAKELTIPPAKPPDMERLMAIAAKYDIEILGPLPG
                     "
     gene            3568..4017
                     /locus_tag="VHM69_14210"
     CDS             3568..4017
                     /locus_tag="VHM69_14210"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2741594.1"
                     /translation="MSEQEENKAVVRRWIEAYNERDLEAEADVLAPGLVVHVSAVPGP
                     LEGLEAWRQFSGSFAEAFPDLRLTVQDIAAERDTVAARVSFHGTHRGEFQGIPPTGKE
                     VAFSSMEFNRVVDGKVQEHWVEIDLLGLMQQLGAIPEPGQPGMREPA"
     gene            4292..4615
                     /locus_tag="VHM69_14215"
     CDS             4292..4615
                     /locus_tag="VHM69_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741595.1"
                     /translation="MMDLDSFLVSLNVYIDEWWKTFHRPNASRKPGRPALLSESEVLT
                     LAILAQWPRFRSEPDFWRFASSHLRSYFPTLCSQTLRLNTRPRKVLEYSTPADTMART
                     VALTG"
     gene            complement(4657..4926)
                     /locus_tag="VHM69_14220"
     CDS             complement(4657..4926)
                     /locus_tag="VHM69_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741596.1"
                     /translation="MRPDAEVVTAEAHEIREIVERYGPDVVICSLPKGGDANCMRAWI
                     ELSLDPTQPTKVRVDGEHSEVINPTLDTLLVIIDEVAQLARTSEL"
     gene            6116..8785
                     /locus_tag="VHM69_14225"
     CDS             6116..8785
                     /locus_tag="VHM69_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741597.1"
                     /translation="MKALHGFERQYRPESGVLRILLFEHNTPDAQLIRTRLSEGGVAH
                     ELDHVQSREAFVAALEQGEVDLILADFALPGLDGLSALRLAMRIRPGVPFIFVSDSLG
                     EEVAIQAIKSGATDYVLKHRLERLRTAIRRAMREVEERRQRERAEEEFARLAFLPRVG
                     PTLIVEATVTGEPVFIDPAAKEQIPGLIELGGRHPALADLASVARKIRESGGQPDNSE
                     VRVDGTLYQRVIFMSPGSDLVRLYVADVTERRRAEETLRESEEQFRWIFEQATVGVAQ
                     VGIDGRWLRVNDKLAEITGYSCDELLERTFQDITHHDELDTDLEQMERLLSGEIATYS
                     REKRYIKKDGSTVWVNLIVSLVRDRAEDPKYFVSAVEDISKRRRAEEALKDSEELYRT
                     VVEQTPENIFIVDLESRRIVEANAALQRSLGYTLEELKGMVIYDVVADDLERVDEITE
                     IILREGTYFVGERKYRRKDGSLLDVEVNVGVVPYGDRRAMCIVAHDVTDHKHSEIALR
                     ESLSVLLALREAGQVLSSTLESEEIVTRLLEIMRNVANLTAAVISRFDHHGNLRVWRS
                     AGLEELWPRVRFTPEAEAARRAVLNSEGRQLFRLRRPGSEGDFLVGLCLPIGTRVSGG
                     VLEAYGSETLAQDDMIEIVNSMTGQAASALENARLYEELGKRERTLQNLVGKLLGAQE
                     EERRRVAYEVHDGLAQVAVAAHQNLQAFARRHVPEEEQARRELDRILKQVRATVSDAR
                     RVIANLRPTALDDLGLAAAVSLEVERLSKEGYHVDYDERLGDERLPGEMEITLFRVTQ
                     EALTNMRKHAGTNWVSVELVRRDSEVHLEVRDGGRGFDPAAVERLGGGPGERVGLQGM
                     RERVSMLGGELEVQSRPNAGTSIAATIPLIRTS"
     gene            8923..9630
                     /locus_tag="VHM69_14230"
     CDS             8923..9630
                     /locus_tag="VHM69_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741598.1"
                     /translation="METGMSPEQVRGRARLAIVDDHDLTRESLQNMLSDEPDIEIVGQ
                     AANGRQALLLCSRLRPDLILMDVRMPEMDGLAATKEIKQRYPETSVMMLTMHENPDYL
                     LEALKAGAAGYILKDAPQEDIIEAVRRVRSGESPLDPELAARLLRRLAIEGGMNRGTL
                     GPRGNAHEVDPLTPRELEVLELMRLGRTNRQIAENLVISLGTSKNHVEHIIAKLGVSD
                     RTQAVVKALEIGILGLS"
     gene            9898..10116
                     /locus_tag="VHM69_14235"
     CDS             9898..10116
                     /locus_tag="VHM69_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741599.1"
                     /translation="MIKVSVEVSGASANFTADVWAESVAQAVDLASACNPGCEAKLLF
                     PIDPDTFFAKASAEGTRLIRSEVLAATG"
     gene            10438..>10782
                     /locus_tag="VHM69_14240"
     CDS             10438..>10782
                     /locus_tag="VHM69_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741600.1"
                     /translation="MCGIVGYAGKERCVEVLMEGLGHLEYRGYDSAGLALQREGRLEQ
                     IREVGRLGNLIRTVDSRNGDLSGVRTGVGHTRWATHGRPSEANAHPHLGGEGTVAVVH
                     NGIIENFADLKEE"
BASE COUNT         2208 a         3061 c         3558 g         1955 t
ORIGIN      
        1 ggtgggggag ctggagacct acgagtcggc gcggctgttc gccgaaaggg cacgccagcg
       61 aaatccgtcc ttcgtgttga ccgccagcaa cgcggaggcc gtctcaaggg tatgccgaca
      121 gttggagggc atgccgctcg ccatagagct ggccgccgca cgtatggagg cgctctcggc
      181 ggagcagctc gcatcgaggc tccatgctcc cctcaaggtg ctcagcagag gcgaacggac
      241 ggcagagccc aggcatcgga gcttgcgggc gacgctcgag tggagccacg agctcctcgg
      301 cgagcacgag caggcgctct tcaggcgact ttccgtgttt gccgggggat ggactcttga
      361 ggcggcagaa gaggtgtgct ccggggatgg catcgaggag ggcgacgtcc tggacctgct
      421 ctccgaactg gtcgtgaggt ctttggtggt gaccgaggca gggggcgaag aggcgctgcg
      481 ctacgggatg ctggaccttg tccgccagta cgcgagggag gagctcgagg agagcgggga
      541 atccgagagc ctccgtagcc ggcacgcctg gtactacctg gcgcttgccg agagggaaga
      601 tgaccaagac gcggaccctg gtgtgaggag atcgcggccg gtagcgtggc tcaggcagat
      661 ggaagccgag cacgccaacc tcagggcggc tctcagttgg tcgctcgatg gggacgccgg
      721 gcagcccgag ggaagacggg tggagctggg gcaaaggctg gcgaccgcgc tattctggtt
      781 ctggcagacg cacgactacc agaccgaggg acgaagctac ctggagaggg ccctctccaa
      841 cagcaccggc acaacgacgg cccgtttgag ggcacaagcg ttggacgcgg ccggcgggat
      901 tgcgggcttt cagggagaca acggagcggc gaaggccctc ctccaggagg ggctggccct
      961 ctaccggcag ctggaggacg aggagggcat cgcatccacc ctcaccgacc tcggaatgat
     1021 cgcggtgggt ggccagcgag aggacatacc cctgagtgcc gtaatggaag agctcgggga
     1081 gctcaagccg tcgctaaaga gccgcaatac gctcgcctgg ctgcacctgg tcgagggtgg
     1141 gatagcggtg ggccggggcg atctggagga ttctgtggcg cactgcgagc ggagcctcga
     1201 gctctttcgc gagatacggg acccgcgggg catcctcagc tgcctgctcc acctcggcat
     1261 gctggcgctg atcggaggta accacgagga agccgtgccg atgctgcggg aaagcctgcg
     1321 cctggcgtgg gacctggatt acacggtggt aatccagtgc tccctctact cacttgcgtg
     1381 cgtggcggcc tctcgggagc agccggtcct cgccgcgcgt ctgtggggga ccgtggaggg
     1441 cctggaggag gcttacggtg tgcgcatagc gtccctgatc ctctctgtca ccgattacga
     1501 gggacgcctg ggcatcgtgc gctcgcagct tggtgaggag gcctgcttgg aagcgtgggc
     1561 ggaggggaaa gcgatggcgc tggagcgggc cgtcgagtac cccctctcgg aggaagagca
     1621 gcaggaatct ccagtgcgcc tcgctgcgtc ggagcggcag ccaccaccgg tcgacggacg
     1681 agcagagagg ctcacccccc gcgagcaaga gatcgctatc ctcgtcgcgc gggggtttac
     1741 caaccgccag atcgcctccg aactcttcat ctccgagcgc acggtggatc accacgtctc
     1801 caacatcttg aagaagctcg acgttagctc ccgcgagcag gtcgcgtctc gcctcggcga
     1861 tcgttagcaa tgccagcctt ccatcgccaa gggaaccctc aaccactacc cgcggcgttt
     1921 taccaaagct cgattcccct gtcctaggac ggatatcaga ggataccgcc gcaggccgtt
     1981 tacgcctcga gacgcttcgc ggttatgggg cgacccttcc ctactgctcg aagataggca
     2041 caagataggc acctcctggg tacctcaggt aacggccagg gaacgcacac cctctacgct
     2101 tgtgcccatg aagcacgcgg cgagcaatcg agaaggaggg cgaccatgaa ccattacctg
     2161 ggcctcgatc cctacgtgat caaggaacgc aacgaggaga tcaggcggga ggtgcgagcc
     2221 ctgcggctcg agggacgcct gcgggacggt cacaggcaga cgccttcgcg tttggtcctc
     2281 ttcgccttga ggagcacatt gcctctgctt cgcagtgtgg ggctagcaga gcggtagatc
     2341 cctatcggga gggggaagga ggtgaacgca ttcgagagat acctacacga tgggggttgc
     2401 gatgcccatc atcccgcccc acagaaagaa gaagggcgag cggtgagaac agggagatgg
     2461 agcagacgac cgctatcggg ggccgatatg aagcggagat acttaggccg ccacctgaac
     2521 cgtagagaca ctgtgccaca tccagacacc cacaaaggag aaggccatga ctacaggctt
     2581 tatcgacgcg gcgagggacg aaggttcctt cgcgacctta gaaggaggtg gacgagcatg
     2641 gttgcgcctg ctcgccagcc aggaggcaag gcacctccgc gagaaagagg aggcggccat
     2701 agaggctgag gattactggg agcaggccga agaggcttgt gccatagatc ccatcgggtg
     2761 agtggcaagg atcaggagac gggacacatg gtatgtcgcg tttggtcctc ttcgccttga
     2821 ggagcacgtt tcccctgctt cgcagtgtga ggctagcaga gcggtagatc tctaccaggg
     2881 gggaaaggag gtaagcgcat tcgagagata tctactatgc gggtggcggt gctgaatatc
     2941 ccgccccaga aaatgaagat ggccaatatg acccgagcga aaggagaaag cgatggatat
     3001 aggagttcaa ccgtacgcgc tggcgaggga aaatggccag gcggtgtggt ttttgggcac
     3061 tctcgtgata gtcaaggcga ccggcgagca gaccggaggc gccttcggcc tcatcgataa
     3121 cctgatgccg gccgggttcg cctcgcctta ccacatgcat cgcaacgagg acgagtcctt
     3181 ctacgtcgtc gagggcgaaa tgactttcta cgtcggagac gagagggtga aagccgagcc
     3241 gggcgcgttc gtctacgggc cgcgcggggt gccccacggg ttcgaggtaa acggaacggc
     3301 gccggccagg atactcctgc aaaactaccc ggctggcttc gaggggttcc ccgttgaagt
     3361 gggcgagccg gccaaggagc tgaccatacc gccggcgaag ccacccgaca tggagagact
     3421 tatggcgatt gcggccaagt acgacatcga gatcctgggg ccgctacccg gctaataaca
     3481 cgttcggtgt agcgtgccgg cacccaacgg ccgctagagc gaacttgagt atcatgagta
     3541 aacacagcga gacacaagga gatagaggtg tcggaacaag aagagaacaa ggccgtcgtt
     3601 agacggtgga tcgaggctta caacgagcgc gacctggagg ccgaagcgga cgtcttggct
     3661 cccggcctcg tagttcacgt ctcggctgtc ccgggccccc tggagggcct ggaggcctgg
     3721 aggcagttca gcggctcctt cgcggaggcg ttccccgacc ttcggctcac ggtgcaggac
     3781 atcgcggccg agagggacac ggtagcggct cgtgtgtcct tccatggtac ccaccgcggc
     3841 gagttccagg gtatcccacc gaccggcaag gaagtggcct tctcgagcat ggagttcaac
     3901 cgtgtagtgg acggtaaggt gcaggaacac tgggtcgaga tcgacctgct cggcctgatg
     3961 cagcagttag gtgccatccc cgaaccgggg cagccgggta tgcgtgagcc tgcgtaggta
     4021 gccgttattc acccgagtgc gggaagagta gcgcgggagc ggggcggacg ctagaaccag
     4081 agccacgcta ttgaaagata cgccatatgg tggatacatc gacgcatctt cgccattgta
     4141 atgcaatatg cgggcccgta ccgcttgtga cgagcagcgg ccgagggagg ttacacccga
     4201 gtactgagac ggctgatgcg taattgtgat cgacgaggaa ggaaccgaga gtctattctg
     4261 gcaccgaacc ttctcccaaa ggcggtgccc cgtgatggac ctcgactcct tcttggtctc
     4321 attgaacgtc tacatagacg agtggtggaa gaccttccac cgcccgaatg cgtctcgcaa
     4381 gcccggccgt cccgctctgc tttctgagag cgaagtcctt accctggcca tcctcgccca
     4441 gtggcctcgt ttccgcagcg agccagactt ctggcgtttc gcttcttcgc acctgcgctc
     4501 ctacttccct accctctgct cccagacgct gaggctaaac accaggcccc gcaaggttct
     4561 cgagtacagc accccggctg atacaatggc acggaccgtt gcgctcaccg gttgaatccg
     4621 cccaatcctt ccgtaagctg ctggagatgc tggtagtcaa agttcgctcg tccgggcaag
     4681 ctgcgcgacc tcatctatga tcacgagcag cgtgtccagc gtggggttga tcacctccga
     4741 gtgttctccg tctacccgta ctttcgttgg ctgggtgggg tctagtgaga gttcgatcca
     4801 ggcccgcata cagttcgcgt cgccgccctt gggcaggctg cagatgacga catccggacc
     4861 gtagcgctcc acaatctccc ttatctcatg tgcctcggcg gttaccactt ccgcgtctgg
     4921 acgcactatc ctgatggtcg ctgcgagcgt ctcccgatag gcgcggaacg cgtcctcgaa
     4981 agcgaccagt atcttgatcc ggattggttg ggattgtatg ctaatttttg attcctggtt
     5041 agataaggca tgtgctccgc gtgtccatgc caatcaacct attccatgtc attcgcggaa
     5101 cgctggagtg ctccctagct caatcctcag gctcaccacc tcgtgatttt gtctcagccc
     5161 agactggagg tcgaaaggaa agtagattga gccggcttcg gcacccggga cagaaccaga
     5221 ttcgctcgcc attggttgcg ctcgcttcct cgtcgtcgaa aaacgtcagc tggcccagtt
     5281 gttcaccgtg gcgcgtaacc tcgcagcgta ccttgtaact acatccgcac aagagctggt
     5341 atgcccaccc ggttgtccta tcttgcccgg caccgatttc gacgccacgt cctccgtcgt
     5401 tgtctaccgg caacggtctc atttggacgg aaccccgtat cgcgacctcc tttggcggag
     5461 ctcgtcctcg cgcagcgagt tctcgccgct cgaggctctt ggcggaggct ttcccaggtt
     5521 aacaaccttc actttcgtgc ctttcctatc tgctggtcct ccgtaccctc ctttactagt
     5581 cgcatcctat acccgcagca ccgtcagcga ccgtgccggg aggcctattt tcggtcgtca
     5641 ggatagaata aatttgcttg cgtcgcccgc gaggccctcc tcgcggcgtg cgccgatgac
     5701 gtgggtttgg tgtacaatag tccgacgaac acttcagtgt atcctggtta ctgcacgatg
     5761 cggaagtatt cggaggtgcc ggcatcggat ccgttagcgt gagacgtatc gtcggtagcc
     5821 acgctggacg ggtgcacgaa tggtgggaga atgagctacc tttaagcttc gcttccgcga
     5881 tacgcggaaa caaggtgtgg aagacaggca tgacagcagt acgctcacct agaactcgac
     5941 ctgaacttag cagcgttgag cgaggagtgg ccagccgagc tgttcgttgt ggcgcgaggc
     6001 gagaagacgg cttccgattg tctcgaccgc cggggcgcgc acccgtcgca ggccctgcga
     6061 acctgggcgc gctcgatctc gacagccatc ggtctagcgc tatgaggcga gaatcttgaa
     6121 agcattgcac ggattcgaaa ggcagtaccg ccctgagtct ggcgtcctgc gcattctcct
     6181 gttcgaacac aacacgccgg atgcacaatt gatccgcact cgactttctg agggcggggt
     6241 cgcccacgag ttggaccacg tacagagccg cgaagccttc gtcgcagcgc tcgagcaggg
     6301 cgaggtcgat cttattctgg ccgactttgc gctaccgggc ttagacggtc tctcggcatt
     6361 gaggctggcc atgcgaatcc gtcccggggt gccgttcatc ttcgtctcgg actcgctcgg
     6421 tgaggaagtt gcgatccagg ccataaagag cggggccacg gattacgtgc tcaagcatcg
     6481 tttggagcgg ctccggacgg cgatccggcg ggctatgcgt gaagtcgagg agcgtcggca
     6541 gcgcgagcgt gccgaggaag agttcgccag gctggctttc ctgccaagag tcggcccgac
     6601 cttaatcgtg gaggctaccg ttacgggcga accggtcttc atcgatcctg cggccaagga
     6661 acagattccg ggtctaatcg agttgggagg gcggcaccct gcactcgcgg atctggcctc
     6721 ggtggcccgc aagataaggg agtctggtgg gcagccggac aatagcgagg tgcgggtcga
     6781 tggaacactc tatcagcggg taatcttcat gagcccgggc agcgacctcg tgcgtctcta
     6841 cgtcgcagat gttaccgaac gccgccgggc agaagagacg ctgcgggaga gcgaggagca
     6901 attcaggtgg atcttcgaac aggcgacggt cggcgttgcg caggtgggaa ttgacggtcg
     6961 ctggctacgg gtgaacgaca agctcgccga gatcacgggt tattcctgcg acgagctgct
     7021 cgagagaact tttcaggaca taacccatca cgacgagctc gacaccgatc tcgagcagat
     7081 ggaaaggcta ctgagtggtg agatagcgac gtactccagg gagaagcgct acataaagaa
     7141 agatggctcg accgtctggg tcaatctgat cgtctccctg gtgagggatc gggcggagga
     7201 ccccaagtat ttcgtctccg ccgtagagga catctccaag cgcagacgag ccgaagaggc
     7261 gctcaaggat agcgaggaac tctatcgcac cgttgtggag cagacgccgg agaacatctt
     7321 catcgttgac ctggagagca gacgcatagt cgaggccaac gcggcgttgc agcgctcgtt
     7381 gggctatacc ctcgaagaat tgaaaggcat ggtaatctac gatgtcgtcg ccgacgacct
     7441 cgagcgcgtc gacgagatca cggagatcat tctaagagaa ggcacgtact tcgtcggcga
     7501 gaggaaatat cgtcgcaaag atggctcgct gctcgacgtc gaggtcaacg tcggcgtggt
     7561 cccttacgga gacaggcgcg ccatgtgcat agtggcccac gatgtcaccg atcacaagca
     7621 ttccgagatc gccctgcgcg agagcctgag cgttctgtta gcattgcgcg aggctggcca
     7681 ggtcttgagt tccacgctcg agtcggagga gatcgttacc aggctgctgg agatcatgcg
     7741 caacgtggcg aaccttacgg cggcggttat aagcaggttt gatcaccacg gaaacctgcg
     7801 cgtctggcga tccgccggcc ttgaggagct gtggccccgg gttcgcttta cgccggaagc
     7861 cgaagcggca cgcagggcgg tgctaaatag cgaagggcga cagctgttca ggctgcgacg
     7921 cccaggctcg gaaggtgact tcctcgtagg tctttgcctc ccgattggca ccagagtaag
     7981 tggcggggtt ttagaggcgt acggcagtga aaccttggcc caagacgaca tgatagagat
     8041 cgtgaacagc atgaccggcc aggctgcgag cgctctcgaa aacgcccggc tctacgagga
     8101 attgggcaag cgcgagcgta cgttgcagaa cctggtaggg aagttgctgg gagctcagga
     8161 agaggagaga cgccgtgtag cctacgaggt gcacgatggc ctggcccagg ttgcggtggc
     8221 tgcacaccag aatctgcagg ccttcgcacg gcggcacgtc ccagaggagg agcaagccag
     8281 gagagaactg gatcgtatcc tcaagcaggt ccgggcgacg gtctcggatg cacgccgggt
     8341 catcgcgaac ttgaggccga cagcgctaga cgacctcggg ctcgcggccg ccgtttcgct
     8401 ggaagtcgaa cgccttagca aggaaggcta ccacgtcgac tacgacgaac gactcggaga
     8461 tgagcgtttg ccgggtgaga tggagatcac gctcttccgc gttacgcagg aagcgttgac
     8521 caacatgcgc aagcacgctg ggacgaattg ggtgagcgtc gagttggtaa ggcgggatag
     8581 tgaagttcat ctagaagtcc gcgacggcgg acgcggattc gacccggccg cagtagagag
     8641 gttgggtggc gggccgggcg agcgggtcgg tctccagggg atgagggagc gagtgagcat
     8701 gcttggtggc gaactcgagg tacagagccg cccaaatgcc ggcacttcga ttgcggccac
     8761 gatccctctg atacggactt catgactcaa ccatcttacg ggcgagacgg gatcgagtga
     8821 tataggaact ggtagccagc cggtcgacgt tacaggagcg cctcgacgca gggtaaggtc
     8881 gcgaatagga ttagcggtta tataaggagg taagcagggg ctgtggaaac gggaatgtct
     8941 ccagagcagg tacgcgggcg ggcgcggttg gccattgttg acgaccacga tctcacccga
     9001 gagagcctcc agaacatgct ctccgacgag cccgacatcg agatcgtcgg ccaggcggcc
     9061 aacgggcgtc aggcgctctt gctctgctcg cgcctccggc ccgaccttat cctcatggac
     9121 gtgcggatgc cggagatgga cggccttgcg gccacgaagg aaatcaagca aagatacccc
     9181 gagaccagcg tcatgatgct gaccatgcac gagaaccccg actacctgct cgaagccctg
     9241 aaggcggggg cggccggcta catactcaag gatgcccccc aggaagatat tatcgaggcc
     9301 gtgcggcggg tgcgcagtgg ggagtccccg ctggacccgg agttggccgc ccggcttctg
     9361 cgccggttgg ccatcgaagg ggggatgaat aggggaacgc tcggacctcg aggtaacgcc
     9421 cacgaggttg accccctgac cccgcgggag ctcgaggttc tagaactcat gaggctagga
     9481 cgtaccaacc gtcagatcgc cgagaacctc gtgataagcc tcggtacgtc gaagaaccac
     9541 gtcgagcaca tcatcgccaa gttgggtgtc tccgaccgga ctcaagcggt ggtgaaggcc
     9601 ctggaaatcg gaatcctggg tctttcctag gcacctgacg aacgaaattt ctggtagacg
     9661 gctgcgcctc tcacggcacg tacgcggccg ctgggatcag atccgctaag cgctcgagag
     9721 gtccgtcggg cgtgcgcgag agtctagtcg agccgaatct actttgacac ccgagaaatg
     9781 tgccgaatgg ctgatgtggg gtacccatca tcggagtgaa catgggataa aaatggcaag
     9841 ccctcctaga gtgaaggcca gatagctcga cgagctagaa cgagcgagag gaggaacgtg
     9901 ataaaggtct cggtggaggt tagcggcgcc tcagccaact ttacggcgga cgtctgggct
     9961 gagagcgtgg ctcaagccgt cgatttggcc agtgcctgca atccaggttg cgaggccaag
    10021 ctgttgtttc ctattgaccc ggacaccttc ttcgcgaaag cttcggcaga gggcacgagg
    10081 ttgataaggt ctgaggtcct tgcagcgacg ggatgagttg gagagcaaga gtacgtcggc
    10141 caggacaaga gcatttcgcg gcagacggca agcaagctcg gtgggatctg cacggcttgc
    10201 ggggtagaga agagtatcaa gcgccaggac tctcgcactt cgggagtcga cgaggaagac
    10261 gttcatcgaa gcattcggcg ggtacgtctc ggcttcgggc ggtcgtcagg acggtgacaa
    10321 atcccggtgg tgacgccggg gacaaagcac cgttcgtgcc cggacagagc accatagctc
    10381 tgtctccttt ctctgcctcc cggcatccca tcggcaaata gacagggagg tacgcccgtg
    10441 tgcggcatag tgggttacgc tggcaaagag cggtgtgtcg aggtgctcat ggagggcctc
    10501 gggcacctgg agtatcgtgg ttacgattct gccgggctcg ccctgcaaag ggagggccgg
    10561 ttagagcaga ttagagaggt cgggaggctc gggaacctca tccgtaccgt tgactctcgc
    10621 aatggggatc tctccggcgt acggaccggg gtgggacaca cccgctgggc cacgcacggg
    10681 cgtcccagcg aggcgaacgc ccatccacat ctcggcggag aaggcaccgt cgcggtcgtg
    10741 cacaacggca tcatagagaa cttcgctgat ctcaaagagg ag
//
LOCUS       DATAIR010000598         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_456070, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000598 DATAIR010000000
VERSION     DATAIR010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456070"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..854
                     /locus_tag="VHM69_14245"
     CDS             195..854
                     /locus_tag="VHM69_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX2741601.1"
                     /translation="MLRVRDCMTKDVVTLGPEASAAEAWSLCREHNIRHVPVVQEGRL
                     AGLVSDRDLRDVRGGGEGRESDTPRWVRLGDMMTRNVVTIHPLDTIEHAARELYERKI
                     GCLPVVADDELVGIITSEDMMRTLIEMVGAQGRGTWVEVEVPNEPGTLAEVTDVIRDR
                     KVGIASIFLGPASRETNRLIVLRLETTNPAGIVATLRDAGYGVTTVESSFVAAKSPYE
                     E"
     gene            complement(1037..>1627)
                     /locus_tag="VHM69_14250"
     CDS             complement(1037..>1627)
                     /locus_tag="VHM69_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741602.1"
                     /translation="FVNTSSETVPGFFFPERPFLPDYVPVDEEHPIRPQDPYATAKYF
                     GELLMDGAIRRSDISCISIRPSWVQHEGNYERNLGPMVRDRSELSAGIWSYIDVYDLA
                     DAIVLAATSDLPGHEVFYIASPDNVGGRPFEAAVREHYGDQIQIKTPLPREDASGISI
                     EKARRMLGYDPKRSWRDYLDDEGRLKPGVKGLFSSS"
BASE COUNT          306 a          475 c          537 g          309 t
ORIGIN      
        1 cgttggccca tcgacctcag cgagcttcag cgcctgctcc gggtcaccga ggctctgcac
       61 ctgccaggct ctcatagcgg atcctccctc cagttcatgg gcttcgagcc taccacagcg
      121 cctgtccagc cggaatggta agctccgctg agaacgtaga aagagcagcc gcggaagagg
      181 acaggagcgt atgtatgctt agggttaggg actgcatgac gaaggacgtc gttacgctag
      241 ggccggaggc gagcgccgca gaggcgtggt ctctttgcag ggagcacaac atccgtcacg
      301 tgcccgtcgt gcaagaaggg cggctcgcgg gcctggtctc ggacagggac ctcagggacg
      361 tcagaggcgg tggggaggga cgcgaatcag ataccccgcg gtgggtgcga ctcggcgaca
      421 tgatgacaag gaatgtcgtc accatacacc cgctcgatac catcgaacac gctgcacgcg
      481 agctttacga gcgcaaaata ggctgtctcc ccgtcgtcgc tgatgatgag ctggtcggca
      541 tcataacctc cgaggacatg atgcgtaccc tcattgagat ggtcggggcg caaggccgcg
      601 ggacctgggt cgaggtcgag gtccctaacg agcctggcac gctcgccgag gttaccgatg
      661 tgatccggga ccgcaaggtc ggcatagcca gcatcttcct cggtccggcc agccgtgaga
      721 ccaaccgcct catcgtgctg cggctggaga cgacgaaccc ggcaggaata gtggcgactc
      781 tgagggacgc cggctacggc gtaacaaccg tcgagtcttc gttcgtcgcg gcgaagagtc
      841 cgtacgagga gtagctcttt gagcgaagtg cggaagtcct gacttccgca cttcgcgctg
      901 tcagcacggc gccttgcagg cgcatttcag ctcgcttcgg gccttcagcc ttcagcttgt
      961 tgccgcgaaa tttgttgatt ccagagcttc gttggctgag ggcgtttgca ggttagatct
     1021 cattgcaagg gccgctctag gaactcgaga acaacccctt tacgccaggc ttcaagcggc
     1081 cctcatcgtc gaggtagtcg cgccaggagc gtttggggtc gtagccgagc atgcgccgcg
     1141 ctttctctat ggagatgcct gaggcgtcct ctcgggggag gggtgtcttt atctggatct
     1201 gatccccgta gtgctcgcgc acggcggcct cgaaaggcct tccgccgacg ttgtcgggcg
     1261 aggcgatgta gaagacctcg tggcctggca gatcactcgt cgcggcgagc actatggcgt
     1321 ctgccaggtc gtagacgtcg atgtagctcc agatacccgc gctcagctcc gagcggtcgc
     1381 ggaccatcgg acccaggtta cgctcgtagt tgccctcgtg ctgcacccag gaagggcgga
     1441 tggagatgca actgatgtcc gagcgccgga tggcgccgtc catcaggagc tcgccgaaat
     1501 acttggcggt cgcgtagggg tcctggggac ggatcggatg ttcctcgtct acgggcacgt
     1561 aatcaggaag aaagggccgc tcggggaaga agaaccctgg taccgtctcg ctggaggtgt
     1621 tcacgaa
//
LOCUS       DATAIR010000599         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_133870, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000599 DATAIR010000000
VERSION     DATAIR010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133870"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHM69_14255"
     CDS             <1..272
                     /locus_tag="VHM69_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741603.1"
                     /translation="GVSFAAVYVNSAPSEDAVPYTMLLDSTGELVSKYNVKRVPRLFV
                     VKNGTVEYTLDYYYDGYEKGISEALDETLAKKPEKGGQSALRGER"
     gene            complement(412..1251)
                     /gene="pyrF"
                     /locus_tag="VHM69_14260"
     CDS             complement(412..1251)
                     /gene="pyrF"
                     /locus_tag="VHM69_14260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2741604.1"
                     /translation="MQALVDAARRKDSVLVAGLDPTPSRFPEELRTLPEVDATLQFCR
                     GILDAVEPYAAAIKLQAAFFERLGPAGMGIYANLIEAAADLDLPVIADIKRGDIGPVA
                     AAYAEAHLGVYGATCVTVNPYMGEDAVLPFLEEARRRSRGGGVFVLVSTSNPSSGRMQ
                     ETTKPPLFEVAAGLVAELGTPGGDYPDAGAVVGVTRPEIGGRVRELLPDALFLSPGYG
                     AQGGEASGVRPLLDRSGGGVLVNSSRDILYAYEGSTSGYREAAGEAARLARDALRRAG
                     ARL"
     gene            complement(1334..1612)
                     /locus_tag="VHM69_14265"
     CDS             complement(1334..1612)
                     /locus_tag="VHM69_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX2741605.1"
                     /translation="MALNKTALIEEIAGKANASKSDAQRFFDAFTEVVTDSLKKGEQV
                     QITGFGKFYVQKRDARQGINPQTKQRINIPASKVPKFTAGNALKDQIK"
     gene            complement(1687..2220)
                     /gene="pyrE"
                     /locus_tag="VHM69_14270"
     CDS             complement(1687..2220)
                     /gene="pyrE"
                     /locus_tag="VHM69_14270"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX2741606.1"
                     /translation="MSRAPDLAPRIREAALLEGDFVLSSGERSNVYVDKYLFSTEPTL
                     LRDVADALAKQIPDDVERLAGVELGAVPLVVAVALATGLPYVIVRKSAKEYGASAGKG
                     IEGNFQEGEKLALIEDVVTTGTQAVRAARNLDEAGVDVVTIVAVLDRREEYGGELGGF
                     PFRALLRMDDLRVVKRN"
     gene            complement(2204..2524)
                     /locus_tag="VHM69_14275"
     CDS             complement(2204..2524)
                     /locus_tag="VHM69_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741607.1"
                     /translation="MVYSPEPMEDTGREQDNVEGLEEKVGALEKVTDSLDEVPDEELV
                     GTLNEAVELLAEINAGIEARLDTAGDESLEIDALLGQVDFGPFDESLEDHERMERSTG
                     EPGA"
     gene            complement(2514..2768)
                     /locus_tag="VHM69_14280"
     CDS             complement(2514..2768)
                     /locus_tag="VHM69_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741608.1"
                     /translation="MNRMHPQTLLFVLLGTVPLVVAGAGVAAFLLMRSGYGVLVWAVV
                     PFVASLLIVATLAFVLSRAAGGPRAQERSSEGRRRDRDGV"
     gene            complement(2816..3076)
                     /locus_tag="VHM69_14285"
     CDS             complement(2816..3076)
                     /locus_tag="VHM69_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2741609.1"
                     /translation="MQRQTKSEVIRRLKSVEGHVRGVQKMVEEGDVTYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAEKDGASEELISDLIKAIERVTK"
     gene            complement(3081..>4266)
                     /locus_tag="VHM69_14290"
     CDS             complement(3081..>4266)
                     /locus_tag="VHM69_14290"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX2741610.1"
                     /translation="SRSMDVYGVTGTNGKTTTSYALHAILAGAYGEEECGLMTTVETI
                     SGGERRPAVRTTPEATEVQGTLAAMLESGVRRVVMEVSSHGIALGRVAGTRFEGAIFT
                     NLTRDHLDLHGSMEAYFAAKRELFYMTVGPRLANAEDEYGRRLAGEVEGVDTFGGTAG
                     ADYRVAEVEATREGTSFALHHHGGVVKLETPLLGPYNVLNVAGAASLALAAGAEEGAI
                     SRAARGMGQVPGRFERVAEAGEHGFEVVVDYAHTDVGLEAVLKVARGVAEAGVDRGRV
                     LCVFGAAGDRDGAKRPMMGRVASQLADMSIITTDDAYSEDPAKIAGEVAAGSDLSRTR
                     VELDRRKAIRRALREARAGDVVVVAGKGHETVQHLPEGDVPFHDATVVGELLAEIGEK
                     GR"
BASE COUNT          741 a         1465 c         1244 g          816 t
ORIGIN      
        1 acggggtctc cttcgcggcg gtctacgtca acagcgcccc gagcgaagac gccgttccat
       61 acaccatgct ccttgactcg accggtgagc tggtctcaaa gtacaacgtc aagagagtac
      121 cgaggctgtt cgtggtcaag aacggcacgg tagagtacac cctggactac tattacgatg
      181 gatacgagaa ggggattagt gaagccctgg acgagaccct cgcgaagaag cccgagaagg
      241 gcggccagag cgccctccgc ggagagagat agatccggag cccacgtgct ccccggaagg
      301 agaagatgac gagagagagc atggtacaga tcgcaggtgt tgtggtcgcc gcggcgacgg
      361 tgctggtcac cgtgctcgcc gtcgtccact tgttctccgc ctgacgggac cttacagacg
      421 ggctcctgcc cggcgcaacg cgtcccgtgc cagacgggcg gcttccccag cggcctccct
      481 gtagcctgag gtcgagcctt cgtaggcgta gaggatgtcc ctgctgctgt tgacgagaac
      541 cccgccgccg ctccggtcga gcagcgggcg tacgcctgat gcctcgcccc cctgtgcccc
      601 gtaaccgggg gagaggaaaa gcgcgtcggg cagcaattcc cgcacccgcc ccccgatctc
      661 aggccgcgtg accccgacga ccgcaccggc atcagggtag tcgccgcccg gagtccccaa
      721 ctccgcaacc aggcctgcgg cgacctcgaa gagcggcggt ttcgtagtct cctgcatcct
      781 ccccgacgat gggttcgagg tcgagaccag cacgaagacg cctccgccac ggcttcgtct
      841 gcgcgcctct tcgaggaagg ggagcaccgc gtcctccccc atataagggt tgaccgtgac
      901 gcaggtggcg ccgtagacgc cgagatgcgc ctccgcgtag gcggcggcga cgggcccgat
      961 gtcaccgcgc ttgatgtcgg ctatcacagg cagatccagg tccgcggcgg cttctatgag
     1021 attcgcgtag attcccatcc cagcgggccc gagacgctcg aagaacgcgg cctgcaactt
     1081 gatggccgcc gcgtagggct ccacagcatc gaggataccc ctgcagaact ggagggtcgc
     1141 gtcgacctca ggcagagtgc gcaactcctc aggaaagcgc gaaggtgtgg gatccaggcc
     1201 cgcaacaagc acgctgtcct tgcggcgcgc cgcgtctacc agggcctgca tcgagatcca
     1261 ggcgttcgag aaaggtgcaa caagatcggc accgcgggct ggtcaccgcg gcaccggata
     1321 agttcttctc tggctacttg atctggtcct tgagcgcgtt gccggcggtg aacttgggca
     1381 ccttggaggc gggaatgttg atcctctgct tggtctgagg gttgatcccc tgcctggcgt
     1441 cacgcttctg cacgtagaac ttgccgaagc cagtgatctg gacctgctcg cctttcttga
     1501 ggctgtcggt cactacttcg gtgaacgcgt cgaagaacct ctgggcatcg ctcttggagg
     1561 cgttcgcctt gccagcgatt tcctcgatca acgccgtttt gtttaatgcc acgtctgttc
     1621 ctccctactt cgcttccaat gctcttcccc cagtttgccg gaggatacca gagacactat
     1681 agcgcctcaa ttccttttaa ctacccgtaa atcatccatt ctaagcaagg cgcggaaggg
     1741 aaatccaccg agttctcccc catactcctc cctgcggtcg agtactgcca ctattgttac
     1801 cacgtctacc ccagcttcgt ccaggttcct ggccgcgcgt accgcctgag tacccgtggt
     1861 gacgacgtct tcgatcaggg ctagcttctc accttcttgg aagttgcctt cgataccctt
     1921 tccggcggag gcaccatact ctttggcaga cttgcggact atgacgtacg gcaaccccgt
     1981 tgcgagggcc acagccacga cgaggggcac ggcaccgagc tccacgcctg cgagtcgctc
     2041 gacatcatcg ggtatctgct ttgccagcgc gtctgccacg tcgcgcagga gcgtcggttc
     2101 ggtcgagaac agatatttat ctacgtagac gttggagcgc tctccgctgg agagtacgaa
     2161 gtccccctcc agaagggctg cttccctgat gcggggcgcg agatcaggcg cccggctcac
     2221 ctgtcgaccg ctccatgcgt tcgtggtcct ccagggactc gtcgaagggc ccaaagtcca
     2281 cctgcccgag gagcgcgtcg atctcgagcg actcgtcacc ggcggtgtcg aggcgggctt
     2341 cgatgccagc gttgatctca gccagtagct caacggcctc gttgagggtc ccgacgagct
     2401 cctcgtcggg cacttcgtcc agagagtcgg tgaccttctc cagcgcgccg accttctcct
     2461 ccagaccttc tacgttgtcc tgttctctcc ctgtatcctc cataggctca ggactataca
     2521 ccatcacgat cccgtcttcg gccctcggaa gacctttcct gagcgcgagg gcctcctgcg
     2581 gcccggctca ggacgaaggc gagcgtcgcg actatgagca gagaagccac gaaaggcacg
     2641 accgcccata ccaggacgcc gtaccccgac ctcatcaaca ggaaagcggc cacgccggcg
     2701 ccggccacta cgagcggaac ggtccccaga aggacgaaga gcagggtctg tggatgcatg
     2761 cgattcatac gtctcccgat cctgaagcag gtaaccgaac gcgcgattgc gtcatctact
     2821 tcgtcaccct ttctatggct ttgatcaggt cactgatcaa ctcttcggag gcgccgtcct
     2881 tttcggccct ctcctcgacg aagtccttta agaggactgt gtttatccgc ctgatggcgg
     2941 agaagatggc gttcgtctgc tggacgatgt cggcgtaggt cacgtcgccc tcctcgacca
     3001 tcttttggac ccctctgacg tggccctcca cgctcttgag ccggcgaatc acctcgcttt
     3061 tggtctgtct ctgcaagctg ctacctcccc ttctccccga tctccgccag tagctccccg
     3121 accaccgtgg cgtcgtggaa gggaacgtcc ccttcaggca ggtgctggac cgtctcgtgg
     3181 cccttgccag cgacaaccac aacgtcaccc gccctcgcct cccgcaaggc gcgccggatc
     3241 gccttccgcc tgtcgagctc gacgcgggtc cgcgagagat cggaacccgc tgcaacctcc
     3301 ccggctatct tggccgggtc ctcggagtac gcgtcgtccg tcgttatgat gctcatgtcg
     3361 gcaagttgag aagctacacg acccatcatg gggcgcttgg ccccgtccct atccccggcc
     3421 gccccgaaca cacagagcac tcgcccccgg tcaacgccgg cttccgcgac cccccttgcg
     3481 accttcaaga cagcctcgag ccccacgtcg gtgtgggcgt agtcaacgac tacttcgaac
     3541 ccgtgctccc ctgcctccgc gacccgctcg aaccgtccag ggacctgccc catacctcgc
     3601 gccgcacgag agattgcccc ctcctccgcc cctgcggcga gcgcgaggga ggcggcccca
     3661 gcgacgttga gtacgttgta aggtccgagc agcggcgtct caagcttcac gacgccgcca
     3721 tgatggtgga gcgcgaagct cgtcccttcc cgcgtcgcct cgacctcggc gaccctgtaa
     3781 tccgcgccgg ccgtgccgcc gaaggtatct acgccctcta cttcccctgc gagccgcctt
     3841 ccgtattcgt cctcggcgtt ggcgagcctg gggcctaccg tcatgtagaa gagctcccgc
     3901 ttggcggcga agtaggcctc catcgagccg tgcaggtcca ggtgatccct tgtcaggttg
     3961 gtgaagatcg ccccttcgaa acgcgtcccg gcgacacgcc cgagcgctat gccgtgcgaa
     4021 gatacctcca tgacgaccct gcgaacgccg gattcgagca tcgcggcgag cgtcccctgc
     4081 acctccgtgg cctcaggggt cgtccttacc gcgggacggc gctccccgcc cgagatggtc
     4141 tcgacggtcg tcatgagccc acactcttcc tccccgtagg ctccggccag gatcgcatgc
     4201 agggcgtagg aggttgtggt cttaccgttg gtgcctgtaa caccgtagac gtccatgctc
     4261 ctcgaa
//
LOCUS       DATAIR010000600         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_410580, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000600 DATAIR010000000
VERSION     DATAIR010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410580"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..870)
                     /locus_tag="VHM69_14295"
     CDS             complement(139..870)
                     /locus_tag="VHM69_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEX2741611.1"
                     /translation="MVEAFAWGFIAASSLLLGALIALRRPIALRPLGLIMAFGSGVLI
                     SSVAYELVEDAFSASGGSAAVGLGLLAGALAFYLGDLAIDRFGGDGRKSAAGGQDEGS
                     ALAIVLGTVLDGIPESIVLGLSLIGGEGVSVAMLAAVFISNLPEALSSTAGLSKSGWQ
                     RGRILGLWTSVTVVCALSSLAGYALFDGAPAATVAFVQSFAGGALLTMLADTMMPEAF
                     KFGGKQVGLFTTLGFALAFLITTIE"
     gene            1381..1971
                     /locus_tag="VHM69_14300"
     CDS             1381..1971
                     /locus_tag="VHM69_14300"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX2741612.1"
                     /translation="MSYTQPQPILPMMAGNWWALLIRGIAAVTFGVAAFLWPGPTLYV
                     LIIFFGAYALVDGLFAIAAGIRGTAGRRRWLLLAEGILGVVAGLVAFFYPGMTALVLL
                     YVIAFWAIFTGILKIAMAISLRREIENEWLMGLGGVLSVLFGIILAVLPGVGLLSLVW
                     LISIYAIVFGVAQIALGFRVRSHRRDTPSREQGQAS"
     gene            2141..3202
                     /locus_tag="VHM69_14305"
     CDS             2141..3202
                     /locus_tag="VHM69_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2741613.1"
                     /translation="MAEIGNPSALLIVNPAAGDAAGFLDGLTKTARERGIRAHVLEPG
                     EDARLAAIAAAEDGSEVLAVAGGDGTVSAVAAVAAERGVALAIVPMGTLNHFARDLGL
                     DLARPLSALDALRAGRERRVDVGRINGRLFINNVSLGLYAEMVGDPGYRHDKLGVATT
                     KLRDALSDPEHRGALRITPPEGGPLEGVLALVVSNNPYEFARLGRLGQRHSLDTGMLQ
                     ISAFDASMLGALVERLLSGTPIGAIDIRPALRHWTSERLQMGILGERVRAGVDGEPTT
                     LEAPLRFSVEPGALRVLVLEGVPEDRRVPPQGAGLRAARTLRQWLRPTLVEQESGSGD
                     PHGRTAPDTVGPQRPESGR"
     gene            complement(3255..>3547)
                     /locus_tag="VHM69_14310"
     CDS             complement(3255..>3547)
                     /locus_tag="VHM69_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX2741614.1"
                     /translation="NSTRWINSRLSELAGLLQNLLSGNLTLVSVQLNEQSRRISAWAA
                     IIAVPTVIASIYGMNFRYMPELYLRFAYPVVVLVMVASCVVLYAVFKRIGWL"
BASE COUNT          649 a         1134 c         1160 g          604 t
ORIGIN      
        1 taagagcatc tgcaatatta ctaaggatgc cgccgagcgt ggtatccagc cagtccagac
       61 atgctgctcc ggccctcctt ctttatgccc ttttgcaccc aagcgtctaa gacgtgcatt
      121 ctcggaactg tgatcccttc actcgatcgt ggtgatgagg aaggccaggg cgaaacccag
      181 ggtggtgaag agcccgacct gcttgccccc gaacttgaat gcctccggca tcatcgtatc
      241 ggccagcatg gtcaacagcg cgccgccggc gaaggactgg acgaaggcga ccgtcgccgc
      301 aggagctccg tcaaagaggg cgtagccggc cagtgaagag agagcgcaca ccacggtgac
      361 cgatgtccag aggcccagga tccgtcctcg ctgccagccg ctcttggaca ggccggccgt
      421 ggaggaaagg gcctctggca ggttggagat aaacacggcc gcgagcatgg cgacgctcac
      481 cccttcaccg cctatcaacg ataggccgag cacgatcgac tcggggatgc cgtccagcac
      541 cgtgccaagc acgatggcca aggctgaccc ctcgtcttgc cctccggcag cgctcttcct
      601 cccgtcccct ccgaagcggt cgatggcgag atctccgagg tagaacgcga gggcgccggc
      661 caacaggccg agccccaccg ccgcgcttcc gcccgatgcg ctgaaggcgt cctcgaccag
      721 ttcgtaggcc acgctgctga tcagaactcc cgagccgaag gccatgatga gccccagggg
      781 gcgtaacgcg atcgggcggc gcagggcgat cagtgctccg agaaggagcg aggaggcggc
      841 gataaacccc cacgcgaacg cctcaaccac cggcagacct caggcgggtc gtggcctcgc
      901 cgcacccttt attctcgaac gagcgggaca agccatacac aactagcatg atgccaccgt
      961 agtgaacccc gcgcctccgt cgggggccca ttcaccgaac ctgcgagagg ataggcgttc
     1021 tcggtctagt cccagcgggg ggatgcccgc ctctcacggc taccgtgcaa cgctttcgca
     1081 acgctcccct aatacactga ctacctacga ataaggaaac gccaacgctg caagtggagg
     1141 tcgagcagag aatgagccct gacgatggcg ccttcgtgtt cgtcgggact tacgcagata
     1201 cccgaagcgc acaagccgac cacgagatgg tttaggagct gcactccggc gggtggtata
     1261 cgccggggct atccagccaa gcgggccatt ttggtcaggg caccaacggt gatgcatgcc
     1321 gaatatacga gggaacgcgt cctacgtggg gatcgagcaa agcggagagg agaggcgaag
     1381 atgagctaca cgcaaccgca acccatactc ccgatgatgg ccggcaactg gtgggcgctc
     1441 ttgatcaggg gtatagccgc cgttaccttc ggggtggctg ccttcttgtg gccggggccg
     1501 accctctacg tgctgatcat cttcttcggg gcctacgccc tggtggatgg cctcttcgcc
     1561 atcgccgccg gcataagggg cacagcggga aggcgcaggt ggctgctgtt ggccgagggg
     1621 atcctcggcg tcgtcgctgg cctcgtggcc ttcttctatc ctggcatgac cgcgctcgta
     1681 ctcttgtacg tgatcgcctt ctgggcgatc ttcaccggca ttctaaagat cgccatggcc
     1741 atctcgctca ggcgggagat agagaacgag tggcttatgg gcttgggcgg ggtactctcc
     1801 gtgctcttcg ggatcatcct ggcggtgctg ccgggggtgg ggctgctttc gctcgtgtgg
     1861 ctcattagta tctacgcgat cgtctttggt gtggcgcaga tcgccctcgg tttcagggtg
     1921 cggagccacc gtcgagatac gccgtcgaga gagcagggtc aggcgagcta gccagacccg
     1981 aggggagtgc gggcgccgtt ggcgacctcg atgattctcc gacccgccaa ccgaacggcc
     2041 tcgagccggc acggcaagct gcgcccccga caacaacgtg gcggcacgat gcagaccgga
     2101 gtcgatcgtt tcccgtcgga cgagcaagcg gcctcagacc gtggctgaga tcgggaaccc
     2161 ctccgctctc ctgatcgtaa atcccgccgc gggggacgcg gcgggattcc tcgatgggct
     2221 gaccaaaacg gcccgtgagc gtgggatccg ggcgcacgtg ctagagccag gggaggacgc
     2281 aaggctcgcc gccatcgcgg cggcggagga cggatccgag gtgctcgcgg tggccggtgg
     2341 cgatgggacc gtatccgcgg tggccgcggt agccgccgag cgcggggtag cgctcgcgat
     2401 cgtgcccatg ggaaccctca accacttcgc ccgcgacctc gggctggacc tcgcgcgccc
     2461 gctcagcgcg ctcgacgcgc tccgtgctgg ccgcgagcgg cgcgtagacg tcggacgcat
     2521 caacggccgc cttttcatca acaacgtctc tttgggtctc tacgcggaga tggtgggaga
     2581 tccgggctac aggcacgaca agctcggcgt ggccacgacg aaactccggg acgccctctc
     2641 cgatccggag catagaggag cgctgcgtat cacgccacca gaaggcggtc cgctcgaggg
     2701 cgtgctcgcc ttggtcgtct cgaacaaccc ctacgagttc gcacggttgg gtaggctagg
     2761 ccaacgccac agcttggaca cggggatgtt gcagatctcg gcgttcgacg ccagcatgct
     2821 aggcgcgctc gtcgagcggc tgctttcggg cacaccgatc ggcgccatcg acatccgtcc
     2881 cgctttacgc cactggacgt ccgagcgtct gcagatgggc atccttggcg aaagggtgcg
     2941 ggccggggta gacggcgagc ctaccacgct cgaggcacct ttgcgcttct cggttgagcc
     3001 cggtgcgctg cgcgtgctcg tgctggaagg ggtgcccgag gaccggcggg tcccgccaca
     3061 aggggccgga cttcgtgccg cccgaaccct gcgtcagtgg ctgcgcccaa ccctggtcga
     3121 gcaagagagc ggaagcggtg atcctcacgg taggacggcc ccggacaccg tgggtcctca
     3181 acgacccgag tcgggtaggt gagctcggcg cgctgggggc actttttgca ttttgtgcac
     3241 cgggtaggcg atcctcacag ccacccgatg cgcttgaaga ccgcgtagag cacgacgcag
     3301 ctcgccacca tgacgagcac caccaccggg taggcgaacc tcaagtagag ctccggcatg
     3361 tagcggaagt tcatgccgta gatgctcgcg attacggtag gcaccgcgat tatggcggcc
     3421 cacgcgctga tcctgcgcga ctgctcgttc agctgaacgc tcaccagggt aaggttgccg
     3481 ctcaagaggt tctgtagaag tccggcaagc tctgacaacc tcgaatttat ccaccgggtg
     3541 ctgtttc
//
LOCUS       DATAIR010000601         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_410628, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000601 DATAIR010000000
VERSION     DATAIR010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410628"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VHM69_14315"
     CDS             <1..1502
                     /locus_tag="VHM69_14315"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2741615.1"
                     /translation="WTRLAPDPLNGGMPDKRVPVQWQVATDEAFANVVREGTTFASPE
                     LAHSVHVEVGGLRPAGEYFYRFRAGSELSPVGRTKSAPAFGASVAEMNFAFVSCQQYE
                     HGYFTAYHHMSEEDLDLVVHLGDYIYEYGPNQYVAPGGNVRTHVGPEIVSLSDYRRRH
                     AQYRTDEDLQAAHAAFPWTVTWDDHEVENNYADEIPEDGQSVEAFILRRAAAYQAYYE
                     HMPLRRSSVPSGPDMQLYRRLTFGNLAEFNVLDTRQYRDDQAAGDGTDPPNPGSLDPN
                     RTLTGETQEQWLLNGLVASGVTWNVLAQQVFFAQRDFDTGDGQRFSMDAWDGYVGSRN
                     RISNFIMVNDIQNPVVLTGDVHNNWACDLKANYDDQSSDTLGVEFVGTSITSGGDGAD
                     TSPSQEAVVAENPHIKFFNGQRGYVRCRLTPEEWRADYRVLPFVKQPGAPIYTRKSFA
                     VEAGNPGLHPAVEQEPLGTRVSSAAIESDAERIRAQEEADRKGRGRQGS"
     gene            1613..>2168
                     /locus_tag="VHM69_14320"
     CDS             1613..>2168
                     /locus_tag="VHM69_14320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX2741616.1"
                     /translation="MARTVEVPDYEVQDVVRVDPSRTALIIGDMQNDFVTEPGTLVVP
                     SAERTVPAIRNLLELARESGMRVVFVQDTHRDGDPEWEIWGEHCREGSWGWQVVDELA
                     PGEDEATIRKPRYDAFYGTQLEHMLRQWGVDTLIICGTVANICVFYTAAGAAMRWFNV
                     VVPRDCISSFNDFDMEASLSQTATL"
BASE COUNT          405 a          705 c          700 g          358 t
ORIGIN      
        1 tatggacccg cctcgccccc gacccgctga acggcgggat gccggataaa agggtccccg
       61 tgcagtggca ggtcgccacc gacgaggcct tcgccaacgt cgtgcgggag gggaccacgt
      121 tcgccagtcc tgagctggcc cactccgtcc acgtcgaggt cggtggcctg cggccggccg
      181 gcgagtactt ctaccgcttc agggccggct ccgagctctc ccctgtgggg cgcacgaagt
      241 ccgcgccggc gttcggggct agcgttgccg aaatgaactt cgcttttgtc tcgtgccagc
      301 aatacgagca tggctacttc acggcgtacc atcacatgag cgaggaggat ctcgacctcg
      361 tcgtccacct cggagactac atctatgaat acggcccgaa ccagtacgtt gcgcccggcg
      421 gcaacgtcag gacgcacgtt gggcctgaga tcgtctcgct ctccgactac cggaggcgcc
      481 acgcacagta ccgcacggac gaggacctgc aggctgcaca cgcggccttc ccgtggaccg
      541 ttacgtggga cgaccacgag gtagagaaca actacgccga cgagatcccc gaggacggcc
      601 agtccgtgga ggccttcatc ctccggcgcg ccgccgccta ccaggcctac tacgagcaca
      661 tgccgctgcg tcgctcctcg gttccaagcg gccctgacat gcagctctac aggcgtctta
      721 ctttcggcaa cctcgccgag ttcaacgtgc tagacacgcg ccagtaccgc gacgaccagg
      781 ccgcgggcga tggcaccgat ccgcccaatc cggggagcct ggatcccaac cgcaccctca
      841 ccggcgagac gcaggaacag tggctcttga acgggcttgt cgcctctggc gttacctgga
      901 acgtcctcgc ccagcaggtc ttcttcgccc agcgcgactt cgatacgggt gacggccagc
      961 ggttcagcat ggacgcgtgg gacggctacg tcggctcccg caaccgcatc tcgaacttca
     1021 ttatggtcaa cgacatccag aatccggtgg tcctgactgg cgacgtccac aacaattggg
     1081 cgtgcgatct caaggcgaac tacgacgacc agagttccga tacgctcggc gtcgagttcg
     1141 tcggaacctc gatcacctcg ggcggcgacg gggcggacac gagcccgagc caggaggccg
     1201 tcgtggccga gaaccctcac ataaagttct tcaacggcca gcgcggctac gtccgatgcc
     1261 gcctcacgcc agaggagtgg cgggccgact acagggtcct gccgttcgtg aagcagccag
     1321 gagcgccgat ctacacgcgg aagtccttcg ccgtcgaggc cggaaaccca ggtctacatc
     1381 ctgcggtcga gcaggaaccg cttggcaccc gcgtctcctc ggcggccatc gagtccgacg
     1441 ccgagcgcat ccgggcgcag gaggaggccg accgcaaagg tcgcggccga caggggagct
     1501 gatagatctt cgcgggacgg gtcaccggac ctgcaggctg gggccaggtg atctattgtt
     1561 ggggcgagag gcggtaaaat cagcgctggt tctgtgatcc aaggaggttc gcatggcacg
     1621 caccgtggaa gtcccggact acgaggtaca ggatgtcgtt cgcgtggacc cgagccgaac
     1681 ggccttgata atcggggaca tgcagaacga tttcgtgacg gagccaggca cgctcgtcgt
     1741 ccccagcgcc gagagaaccg taccggcgat acggaacctg ctggagctgg cgcgggagtc
     1801 cgggatgagg gtggtcttcg ttcaggacac ccacagggat ggtgacccgg agtgggagat
     1861 ctggggcgag cactgccgcg aggggagctg gggctggcag gtggttgacg agctcgcccc
     1921 cggtgaggac gaggcgacta tccgcaagcc acgctacgat gccttctacg gcacgcagct
     1981 ggagcacatg ctgcgtcagt ggggcgtcga caccctgatc atctgtggca ctgtcgccaa
     2041 tatctgcgtc ttctacacgg ccgctggcgc cgcgatgcga tggttcaacg tcgtagttcc
     2101 cagagactgc atctccagct tcaacgactt cgacatggag gcatcgctga gccagacggc
     2161 tactttgc
//
LOCUS       DATAIR010000602         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_41136, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000602 DATAIR010000000
VERSION     DATAIR010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41136"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..561)
                     /locus_tag="VHM69_14325"
     CDS             complement(148..561)
                     /locus_tag="VHM69_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2741617.1"
                     /translation="MNWKIEVVTIPVSDLDRSIEFYAEKVGFNVDIDFKVSDEVRLVQ
                     LTPPGSACSIHLGKNTVNMAPGSIDGVFLVVPDVRAAHAELAGRGVEVGEIQIFEGGR
                     YRPAREGEDLNNVGCIFFNDPDGNRWCVQQIPPHD"
     gene            complement(585..1151)
                     /locus_tag="VHM69_14330"
     CDS             complement(585..1151)
                     /locus_tag="VHM69_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741618.1"
                     /translation="MRKLVAVELVSLDGVMESPEEWAFPYTDDEMNEANAAGMAASDA
                     LLMGRKTYEGMVAFWPNQPGGTPMVDYINSVRKFVVSTTLQDNLEWNNSSLIGGGEGN
                     VAEEIAQLKRQPGKAITVLGSGVLVRTLLREGLLDELTLMVHPIVLGSGKRLFDDGDP
                     KSLELVDATAFGSGVVSLVYRPSAGQGS"
     gene            complement(1186..2298)
                     /locus_tag="VHM69_14335"
     CDS             complement(1186..2298)
                     /locus_tag="VHM69_14335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2741619.1"
                     /translation="MSELQQQIQEAIDGLVETGAERGLQVAVYRDGEPVVEAVAGVAD
                     HETGRPVTPDTPIYCYSVGKGATSTVAHVLVERGLFDYDTPVVELWPEFGAHGKEAAT
                     VRHVLTHTVGVPGIPEDTTPEDLCDWDKMCDAIAEAEPWWEPGTKVAYHAYPFGYIVG
                     EIVRRTTGKPISQVLREDVAGPLGIADEIYFGVPESELGRLARLEDAEGSAEFLASMP
                     DDAPFFKLGPRTVTPTAEFGNRTDILMADIPAGGKMSARAVARMYAALMDEVDGVRLV
                     SPERLREISAGSFTGIDQIMGFPSTWALGYSVGRIGADPQETQSVLGIGGVGGSFACA
                     DTATGVAWAVTKNRLTADFSAAEQISGIVANATAES"
     gene            complement(2335..2949)
                     /locus_tag="VHM69_14340"
     CDS             complement(2335..2949)
                     /locus_tag="VHM69_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741620.1"
                     /translation="MRKLIVCNAVSLDGYYEGPGKNVMALFDYRWEAYPTDESFDAYN
                     AERLRAADTLLLGRVSYEGFKGFWPPVADDPDATPFQREISRLDNAIDKVVISDSLTA
                     EETAPWHNTRIISRADAREQISELKREAGRDILVFGSRTLWKGLLAHDLVDEIHLMIG
                     PVVLGAGTPVFDGKPPVSLRLIDTRTWDGSGNVLVQYEVRRRTT"
     gene            complement(2961..3743)
                     /locus_tag="VHM69_14345"
     CDS             complement(2961..3743)
                     /locus_tag="VHM69_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741621.1"
                     /translation="MTLLLQDRNAVIYGAGGAIGGAVARAFAREGANVFLAGRTLESL
                     DRVADEIRSSGGSAETDQVDALDDQAVDEHVGEIVERAGSIDVSFNAISIRDIQLIPL
                     VEMSSEDFVLPISTGATTHFLTATSAARHMAERGSGVILTLSASAVRAYVPGVYVGGF
                     GIACTAIEALTKQLAAELGPHGIRSVCLRLEGIPESWEGASTEDWSAPTEEIEAAMKA
                     RSLLGRVTTLADVGNAAAFLASDLAGATTGTVFNLTSGTVVD"
     gene            complement(3907..>4752)
                     /locus_tag="VHM69_14350"
     CDS             complement(3907..>4752)
                     /locus_tag="VHM69_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2741622.1"
                     /translation="DFRPKEVTVNESRTGALKVPGATLHYEVCGSGRVLLLIPGGPAD
                     AGGFAPIRDVLSDRYTVVTYDPRGLSRSPFDGEAQDTTVQTFAQDAHHLLAEFGNEPA
                     YVLGDSGGALVGLELVSQHPEQVRLLVANEPPLVRLLDDADEHARFGREIYDTYRSEG
                     IGPAMGKFAAGTGLDQDEPWPVDPSPELAEAMARMQVNMDFFLGHMWLPLGDYAPDIS
                     RLMSRPITVAVGEASEGQLAYRAAVKLAEQLGSEPIVFPGGHSGTITQPEAYAGRLHE
                     VLSTS"
BASE COUNT          854 a         1559 c         1427 g          912 t
ORIGIN      
        1 gtcgccgctc gaataccagt cgaaaaggcg catcccaccc tcgccgagcg gatgctcggg
       61 gccgtcgttc ggtcccgcga taaagccgtc aagcgacatc gtgagccccg tcgctacttt
      121 ccccatgttc gttctcctat ctacgaccta gtcgtggggt ggtatctgtt gcacgcacca
      181 gcggttgccg tcggggtcgt tgaagaagat gcacccgacg ttgttgagat cttccccctc
      241 cctggccggg cggtacctgc ctccttcgaa gatctgtatc tcgcctacct cgacgccacg
      301 cccggccagc tcggcgtggg cggcgcgcac gtcagggacg accaggaaca cgccatcgat
      361 ggagcctggc gccatattca cggtgttctt tccgaggtgg atcgaacagg cagagcctgg
      421 aggggtcaac tggacgagac ggacctcgtc gctgaccttg aagtcgatgt ccacattgaa
      481 gccgactttc tccgcgtaga actcgatgga gcggtctaag tcggataccg ggatcgttac
      541 gacctctatc ttccagttca tgtcgctctc ctttctgtag tctttcaaga accctggccc
      601 gccgaaggcc ggtagacgag agatacgact cccgaaccga acgcggtcgc gtccacgagc
      661 tcgagtgatt tggggtctcc gtcgtcgaag agacgcttcc cgctcccgag gacgatggga
      721 tgcaccatga gcgtcagttc gtcgagcaat ccctcacgca gcagcgtccg cacgaggacg
      781 ccgcttccga ggacggtgat cgccttgccc ggttgccgct tgagttgggc gatctcttca
      841 gcgacgtttc cttcgccacc cccgatcaga ctagagttgt tccactccag gttatcctga
      901 agtgtcgtcg agacgacgaa cttacgcacg ctgtttatgt agtccaccat cggcgtgccg
      961 ccaggctggt tgggccagaa ggcgaccatc ccttcgtacg tttttcgtcc catcagcaag
     1021 gcgtcagacg cggccattcc cgccgcattc gcctcgttca tctcatcatc ggtgtagggg
     1081 aaagcccact cttcaggcga ttccatgacg ccgtccaacg acaccagctc caccgcgacg
     1141 agtttcctca cgttgatcct cctttccctg gctttataac gcgggctagc tttcggctgt
     1201 ggcattcgcg acgatgccgg agatctgttc cgcggctgag aaatcggcgg tgagcctgtt
     1261 cttcgtcacg gcccacgcga cgcccgttgc ggtgtcggcg cacgcgaagc tcccgccgac
     1321 gcccccgatt cccaacacgc tctgggtctc ttgcgggtct gctccgatac gaccgaccga
     1381 gtaaccgagc gcccaggtgg atgggaagcc catgatctgg tcgatcccgg tgaacgaccc
     1441 tgcagatatc tcgcgcagcc gttcgggcga gacgagccgt acgccatcaa cctcgtccat
     1501 cagggcagcg tacatgcgtg ccacggcccg cgccgacatc ttgccgccgg cggggatgtc
     1561 cgccatcagt atgtcggtgc ggttgccgaa ttcggctgtc ggggtcacag tccgcggccc
     1621 cagcttgaag aacggcgcgt catcgggcat cgaggcgaga aattccgcgc tgccttcggc
     1681 gtcctccagc ctcgccaacc tgcccagttc cgactcgggg actccgaagt agatctcatc
     1741 ggcgatgccg agcggaccgg ctacgtcctc gcgaagcacc tgcgagatag gcttgcccgt
     1801 agtccggcgt acgatctccc ctacgatgta gccgaacgga tacgcgtgat aggcgacctt
     1861 cgtgccaggc tcccaccagg gctcggcctc agcgatcgca tcgcacatct tgtcccagtc
     1921 acacaggtct tcaggcgtcg tgtcctccgg tattccaggc acgcctaccg tgtgcgtaag
     1981 gacgtggcgc acggtcgcgg cctccttgcc gtgggcgccg aactcgggcc acagctctac
     2041 tacgggagtg tcgtaatcga acaagccacg ctcgacgagc acgtgcgcca cggtcgaggt
     2101 cgcgcccttg ccgaccgaat agcagtagat aggcgtgtcc ggggtgacag gacgcccggt
     2161 ttcgtggtcg gcgacgccgg cgacggcttc tacgacaggc tcgccgtccc tgtagacggc
     2221 gacctgcagt ccccgctcgg cgcccgtttc cacgagcccg tcgatcgctt cctggatctg
     2281 ttgctgaagt tcactcacgg acggttcctt tcttgtcggc ggaacaaacg agagtcatgt
     2341 cgttcggcgg cggacttcgt actgcacgag tacgttccct gagccatccc acgtgcgtgt
     2401 gtcgatgagc cgaagcgaaa caggtggctt gccgtcgaat accggcgtgc ccgcgcccaa
     2461 tacgaccggg ccgatcatca ggtgtatttc gtcgaccaga tcatgggcca gcagaccttt
     2521 ccagagtgtg cgactgccaa agaccaggat gtctctgccg gcctcgcgtt tgagttcgga
     2581 gatctgctcg cgagcgtcag cgcggctgat gatgcgtgtg ttgtgccagg gtgccgtctc
     2641 ttccgcggtc aggctatccg agatgacgac tttgtctatg gcgttgtcga gccgagatat
     2701 ttcccgttgg aagggcgtgg catcgggatc gtccgccacc ggcggccaga aacccttgaa
     2761 accctcgtat gaaacacgcc caagcagcag cgtatcggcc gcgcgcagcc gttcggcgtt
     2821 gtaggcatcg aagctttcgt ccgtcgggta agcctcccaa cgatagtcga acagggccat
     2881 gacatttttt ccgggcccct cataatagcc atccagtgag acggcgttgc agacgatgag
     2941 tttacgcatc acacacctac ctaatctact accgtaccac tggtcaggtt gaagacggtg
     3001 ccggtcgtcg cgcccgccag gtccgaggcc aggaacgccg ccgcgttgcc cacgtcggcg
     3061 agcgtcgtca cccgccccag caaggaacga gccttcatgg cggcctctat ttcttcggta
     3121 ggagccgacc agtcctcggt cgaggctccc tcccagctct cggggatccc ttcgagccgc
     3181 aggcagacgg aacgaatacc gtgcggaccc agttccgccg cgagttgctt cgtgagtgct
     3241 tcgatggccg tacacgcgat cccaaaaccc ccaacataga cgccggggac gtacgcgcgg
     3301 acggccgagg cggagagagt caggatcaca ccggaacccc tctcggccat gtgccgcgcg
     3361 gccgacgtgg ctgtcaggaa atgcgtagtc gcgccggtcg agatcgggag cacaaaatcc
     3421 tctgaagaca tctcgacgag cgggatcaac tggatgtcac ggatcgagat ggcgttgaat
     3481 gagacgtcga tgctgccggc ccgctccacg atttcaccaa cgtgctcgtc gacggcctgg
     3541 tcgtcgaggg cgtccacctg atccgtctcg gctgatcctc cagaggagcg tatctcgtcg
     3601 gcaaccctgt caagactctc aagggttcga ccggcgagga agacattcgc accctcgcgg
     3661 gcgaaggcgc gggcaaccgc tccgcctata gctcctcccg caccatagat cacggcattc
     3721 ctgtcctgaa gtagcagcgt catttattcc tcctctcgcc gccggccggt gggcgtgaga
     3781 agtctgcgga tgagacgcgg agatccacgc cccgcaccgc acagatcact gtcttcaacc
     3841 tgcctcctcc actgtactcc cacactagta tgggaaagcc cgcggtcaat ccggcacgca
     3901 gggcgctcag gaggtgctga gcacctcatg caggcggcct gcatatgcct cgggttgggt
     3961 aatggtccct gaatggccgc cgggaaagac gatgggttcg ctccccagct gttcggccaa
     4021 cttcacggcg gcgcgatacg cgagttgccc ctccgaagcc tcgccaacgg cgaccgtgat
     4081 cgggcgcgac atgagccggg agatatcggg cgcgtagtcg cccaacggta accacatgtg
     4141 cccgaggaag aagtccatgt tgacctgcat acgggccatc gcctcggcca gctccggtga
     4201 cgggtccacg ggccacggct cgtcctggtc cagccctgtc ccggcggcga acttgcccat
     4261 agcaggaccg atcccttcgg agcgataggt gtcgtagatc tcccgcccga acctggcatg
     4321 ctcgtcggcg tcgtcgagca ggcggaccaa cggcggctcg ttggcaacca gcaatcgaac
     4381 ctgctcgggg tgctgggaca ccagttcgag cccgaccagg gcacctccgc tatcgccgag
     4441 cacgtatgcg ggctcgttcc cgaactctgc gagcagatga tgcgcatcct gcgcgaaggt
     4501 ctggacggtc gtgtcctgag cttcgccgtc aaatgggctg cgggacaatc ccctggggtc
     4561 gtaggtgact accgtatagc ggtcagaaag tacgtccctg ataggtgcaa acccaccggc
     4621 atccgcgggc ccgcccggga tgagcagcaa gacacggccg ctgccgcata cctcgtagtg
     4681 cagcgtcgcg ccagggacct tcagcgcgcc ggtgcgagac tcattcacgg tgacctcctt
     4741 tggtctgaag tc
//
LOCUS       DATAIR010000603         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_41152, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000603 DATAIR010000000
VERSION     DATAIR010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41152"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHM69_14355"
     CDS             complement(<1..569)
                     /locus_tag="VHM69_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2741623.1"
                     /translation="MPAEDGTRLGVDVGGTFTDLVALGGGRLITTKVPSTPRDQSEGV
                     MNAIEASEIDAGAVVALAHGMTVATNALLERRGARTALVTTEGFRDVLEIARQNRPSL
                     YDLAQDRPPALVPRGLRFTIKERMGPDGVLAPLDEASLNETVSAVREAEVDAVAVCLL
                     FTFAHPEHEERVGEALREALPDVHVSLSS"
     gene            complement(559..1881)
                     /locus_tag="VHM69_14360"
     CDS             complement(559..1881)
                     /locus_tag="VHM69_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741624.1"
                     /translation="MQSRAHGTETAGEPPLRKAVVAAAIGNGVEWFDFAVYGFLATYI
                     GLNFFPNEDPTVSLLSTFAVFAAAFFTRPLGGLFFGPLGDKIGRQKVLATVILLMSAS
                     TFLIGLLPSYQQIGLLAPALLVVLRCLQGFSAGGEYGGGAAFLAEYAPDERRGFIVSF
                     LVWSVVIGFLLGSASATVLSSVMSEDAMSAWGWRIPFLLAGPLGLIGLYIRLRLEDTP
                     DFRELEEAGEVSSSPLRETIAHNWVPILQIAGLVIIHNVGFYTVFTYLPVYFTTELGF
                     STTASFASTTVAGLVALALIPPLGALSDRVGRKPLLISSCVLFAVLTYPLFLLMNTGS
                     LSAAVAAHVGLAAFESLFVCCSLAAGAELFATKVRYGGFSIGYNFSVAIFGGTAPYIA
                     TFLVDRTGNPLSPAYYVIIAAVLTLVTVLTMRETAHAPLAQTTAADAG"
     gene            complement(2112..>3201)
                     /locus_tag="VHM69_14365"
     CDS             complement(2112..>3201)
                     /locus_tag="VHM69_14365"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741625.1"
                     /translation="PGAYDGLPTDNGLSDLDTTGRAGSIDAGDNKILVAPVDLGGHER
                     GYLSAPLVAGTLTALALRTLEQGAVIFALYAIRERATRETEDRIKGDLLADLLADRFR
                     DESEARERARHLGLDFSDAPLRVLILGHEPLDAYLERRELDTRSAGPLRARLLSLARS
                     FATGATPPGIAGLDRDHPVVLLPLETGQDSRTSAERLLRLIRDDLPGLRARVAVSAPF
                     TAPKDLAGIHKEAVSLLELADHLDAAEDVLCHDDWKIYGLLLRSSDREGLLETSHRVL
                     DPLLSQDRSADLLATLETYLDNDLSPTRTAAALYVHTNTVKYRLGKLANIMDLDAQTL
                     SGALTLKVALMVRHLDPEGFDAAVTPPD"
BASE COUNT          596 a          938 c         1120 g          547 t
ORIGIN      
        1 tcgctggaga gggacacgtg cacgtcgggg agcgcctcgc gcagtgcttc gccgaccctc
       61 tcttcgtgtt cgggatgagc gaaggtgaag aggaggcaga cggcgacggc atcgacctcg
      121 gcctcgcgga cggctgagac tgtttcgttc aggcttgctt catcgagcgg ggcgagcact
      181 ccgtcaggtc ccatcctctc ctttatggtg aatcgcagcc cgcgcggtac gagggcggga
      241 gggcggtcct gcgccaggtc gtagagcgag ggccggttct ggcgggctat ctccagcacg
      301 tcccggaaac cctcggtcgt cacgagggcg gtacgggccc cccggcgctc cagcagggcg
      361 ttcgtcgcta ccgtcatgcc gtgggcgagg gcgacgaccg ctccggcgtc gatctccgag
      421 gcttcgatgg cgttcatcac gccctctgac tggtcgcgcg gggtggaggg gaccttcgtg
      481 gtgatcaggc gccccccacc gagggcgacg aggtcggtga acgtgccgcc gacgtccacc
      541 ccgagccgtg tgccgtcctc agccggcatc ggcggccgtg gtctgggcga ggggagcatg
      601 ggcggtctcg cgcatcgtca ggacggtgac gagggtgaga acggcggcaa ttatgacgta
      661 gtaggcgggc gaaagtgggt tgcccgtgcg gtcgacgagg aacgttgcta tataaggcgc
      721 ggtcccgccg aagatagcga ccgaaaaatt gtagccgatg gagaagccgc cgtaacgcac
      781 cttggtggcg aacagctctg cgcccgccgc cagagagcag cacacgaaca gggattcgaa
      841 ggcggcgagc ccgacgtggg ccgcgaccgc cgcagaaagg cttcctgtgt tcataagcag
      901 gaacagcgga tatgtaagga cggcgaagag cacgcaagag cttatcagta gcggcttgcg
      961 tcccacgcgg tcagagagcg ctccgagcgg tgggatcagg gccagcgcca ccagtccggc
     1021 caccgtggtc gatgcgaacg acgccgtcgt cgagaaaccg agttcagtcg tgaagtagac
     1081 aggcagatag gtgaagacgg tgtagaagcc tacgttgtgg atgatgacga ggcctgctat
     1141 ctgcaggata gggacccagt tatgggctat cgtctccctg agcggtgagc tggagacctc
     1201 gcctgcttcc tcgagctccc tgaagtcagg cgtgtcctcc agcctgagtc gaatgtagag
     1261 cccgatcaat ccgagtggtc cggcgagcag gaacggtatg cgccagcccc acgcgctcat
     1321 ggcatcttcg gacatgacgg aagagaggac ggtcgccgac gcggagccga gcaggaagcc
     1381 gatgacgacc gaccacacta gaaagctaac tatgaagccc ctccgttcgt caggggcgta
     1441 ctcggccagg aacgcggcac cgccgccgta ctcaccgccg gccgaaaagc cctgcaggca
     1501 gcgcaggacg acgagcagcg ccggggcgag gaggcctatc tgctggtagg aaggcagaag
     1561 gccgatcaag aacgtggagg ccgacatcag caggatcacg gtcgcaagca ccttctggcg
     1621 cccgatcttg tccccgagcg gcccgaagaa cagtccacct aatggccgcg tgaagaaggc
     1681 agcggcgaac acagcgaacg tgctcaatag cgaaaccgtc gggtcctcat tggggaagaa
     1741 gttcaacccg atgtacgtgg cgaggaaccc gtagaccgcg aaatcgaacc actccacgcc
     1801 attcccgatc gccgccgcga ccacggcttt ccgaagtggc ggctctcccg ccgtctctgt
     1861 gccatgtgct ctggattgca tttttccctc ctgtcccgct tacccttcgc cggcgtggac
     1921 ctaatcgtag agtcccccag aactccccgc tacggccctc cggatagaga cctccggcgt
     1981 gcaatgcctg gaggctacct tcctctacaa gtgcttactg tagcgtgcgg aagcgaagaa
     2041 ttgcatgtaa aaacgtccaa taaagcccct gtccatttgt tgaaagagcc aattcgggcg
     2101 tgctgagacg ttcaatcggg gggtgtaacg gcggcgtcga agccttcggg gtcgaggtgg
     2161 cggaccatga gtgccacctt gagggtaaga gcgcctgaga gggtttgtgc atcgaggtcc
     2221 atgatgttgg cgagcttgcc cagccggtac ttcacggtat ttgtgtggac gtagagtgcg
     2281 gctgcggtcc gggtggggct gaggtcgttg tccaggtaag tttcgagcgt ggcgaggagg
     2341 tcagcgctcc gatcttgaga gaggagcggg tcgaggacgc ggtgggaggt ctcgagcagt
     2401 ccttctctgt ccgaggagcg gaggaggagg ccgtagatct tccagtcgtc atggcagagc
     2461 acgtcttcgg cggcatcgag atggtcggcg agttcgagca gtgagaccgc ttccttgtgg
     2521 atgcccgcga ggtctttcgg ggccgtgaac ggggcgctaa cggcgacgcg ggcgcgcagg
     2581 ccgggtaggt cgtctctgat aagtcgcagg agccgttctg cggaagtgcg ggagtcctgg
     2641 cctgtttcaa gaggcagaag cactactgga tgatccctgt caagaccggc tattccgggc
     2701 ggagtagcgc cggtcgcgaa gctcctggcg agggagagga gcctggcgcg cagaggtccg
     2761 gcggatctcg tgtccaactc acggcgttcc aggtaggcgt cgagcggctc gtgaccgagg
     2821 atgaggaccc tgagcggcgc gtccgagaag tccaggccga ggtgacgcgc ccgctcccgc
     2881 gcctctgact cgtccctgaa gcggtcggca aggaggtcgg cgaggaggtc gcccttgatc
     2941 ctgtcctccg tctccctggt tgcgcgctcc ctgatggcgt agagggcgaa gatcacggcc
     3001 ccttgctcca gcgtgcggag ggcgagggcc gtcagtgtgc cggctacgag cggcgcagag
     3061 agatacccgc gctcgtgacc acccaggtcg acgggcgcga cgagaatctt attgtccccc
     3121 gcatcgatgc ttccggcgcg gcccgtcgtg tcgaggtccg aaagaccatt gtcggttggg
     3181 aggccgtcgt aggctccagg g
//
LOCUS       DATAIR010000604         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_456321, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000604 DATAIR010000000
VERSION     DATAIR010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456321"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VHM69_14370"
     CDS             <1..395
                     /locus_tag="VHM69_14370"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2741626.1"
                     /translation="DEAAADYEEQLREFFGGLPQFDLILLGIGGDGHTASLFPETPAL
                     EVRDRWVVANPVQKLDTIRTTLTVPVINAARSVVFLVAGEDKAEALKEILEGDADPRE
                     YPATLVRPPAGPDWMLDRAAARLLSEPL"
     gene            658..1212
                     /locus_tag="VHM69_14375"
     CDS             658..1212
                     /locus_tag="VHM69_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741627.1"
                     /translation="MRNAATFALPTRTLALFLGALLIAVTVFASATSAQAKKPGSGTG
                     SLPVTGTLSDGGSFEGTISDLNASVNDAGDLVVSGVLNGTATDAAGDVTQITDQAFTT
                     TATLDQGGACDILFLDLGPISLDLLGLTVDLSQITLDVNAVPGAGNLLGNLLCAVAGL
                     LDNTGGGATNGIANLLNQIFSLLG"
     gene            1756..2130
                     /locus_tag="VHM69_14380"
     CDS             1756..2130
                     /locus_tag="VHM69_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741628.1"
                     /translation="MGEIQDINVVYNADRDILKVTGKLVGTATKANGVDRAVSERFNT
                     KAVAQQSPTSCKILDLDIGRIHLDLLGLVVDLAPIHLDVTAVPGAGNLLGNLLCAVAG
                     LLDPNNSLANFLDGLLRALYPL"
     gene            complement(2466..>2759)
                     /locus_tag="VHM69_14385"
     CDS             complement(2466..>2759)
                     /locus_tag="VHM69_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741629.1"
                     /translation="VVGFPAAPAIWGLRVLDKLKLSPLYKWVYETASKDSFVSIEKAE
                     RQLGYAPKFSNKEALIRNFRWYLENLKKFENASGVSHRVPWKQGAVGALKRFF"
BASE COUNT          545 a          816 c          820 g          578 t
ORIGIN      
        1 cggatgaagc ggcagcggac tacgaagaac agttgcggga gttcttcgga ggacttcctc
       61 agttcgacct gatcctgctg ggcataggcg gcgacggcca caccgcaagc ctcttcccgg
      121 agaccccggc gctggaggtc cgggaccgat gggtagtcgc caacccggtg cagaaactag
      181 atactatccg aaccacacta accgtccccg tcataaacgc ggcacgctcg gtcgtctttc
      241 tcgtcgccgg tgaggacaag gcggaggcgc tgaaggagat cctcgaaggc gacgcagacc
      301 ctcgcgagta tcccgccacg ctagtccggc cgcccgccgg ccctgattgg atgctggatc
      361 gcgccgccgc caggctgctc tctgagcccc tgtagatatc tccaaatcca accagtcgat
      421 ctggccgggg tgcaactaac attgcacgtc tcgtgtcaga tgtagtgcgg gcccgcatct
      481 cgatgggagt agtgacgtga aagctatccc ccggaggctg cgggcccgtt ctctcatttc
      541 tacccatctg gggccttaag taacagtttg ataacagttt gctctacttt gtttgaaggc
      601 tgtgccaaac gggtacatgc tcgatcgacg tgaaagctac ctcacaggag gtaccagatg
      661 aggaacgcag ctacatttgc cctgcccacg cgcaccttgg cactctttct cggagcactg
      721 ctcatagcgg tgaccgtatt cgcgagtgct acaagcgccc aagccaagaa gccgggcagc
      781 ggtacgggct ccctgcctgt caccggcact ctctccgatg gcgggtcatt cgagggaact
      841 atctcggatc tcaacgcctc ggtgaacgac gcgggggatc tggttgtgag cggcgtgctc
      901 aacgggacgg cgacggacgc agctggtgac gtgacgcaga tcaccgacca ggcgttcaca
      961 actactgcga ctttggatca gggcggtgcg tgcgacatcc tgttcctcga tctggggccg
     1021 atatccctcg accttctagg tctgacggtg gacctctcgc agattacgct cgacgtaaac
     1081 gctgttccgg gtgcaggcaa cctgctcggc aacctgcttt gcgcggtcgc cggcctactc
     1141 gataacacgg gcggcggcgc cacgaacggc attgccaacc tgcttaacca gatcttcagc
     1201 cttctcggtt aaagccggtt atctagggta actcgaaggc gggaggagaa ttctcctccc
     1261 gcctttttgt gtgcccggga cacgtgcgag gtaaggggac gcttgagcgc cctcgctggt
     1321 ggtcatgtac aacggggttg agggattagc gctctcttgc atcgaccgcg ctggccatga
     1381 acacatacag ggcagtaatt cgcgagacgg aactctcccg gagggttgga tcggctgctg
     1441 gaacgggcgg ctgcgaaagc tatcgagacg actcctcaaa tgcttgttac acggtttacg
     1501 ttacttgctc aacggggtaa agctaccgtg acgtgaaagc tacctcagag gaggtatagc
     1561 gatgaaggca acgcgtgtcg cgaagaaagg ggtgttggaa tgctaaatgc cccgacccga
     1621 tccatccggc tggttgcgct gtttgccgca gcatttgcgc ttctggctct gaccatcatc
     1681 atgcccagcc cttcaaaggc gctaagtgtc ggtgatgttc ctgtacgcgg cccgctttcc
     1741 gatggcggca ggttcgtggg agagatccaa gacattaacg tggtgtacaa cgctgaccgg
     1801 gacattctca aggtcaccgg gaagctcgtg ggcacggcta cgaaagcaaa cggtgtcgac
     1861 agggctgtct ctgagaggtt caataccaag gctgttgccc agcaatcgcc gacctcgtgc
     1921 aagatccttg acctcgatat aggtcgtatc catcttgacc tgctcgggct ggtcgttgac
     1981 ctcgcgccga ttcacctgga cgtaacggca gttcctggcg cgggtaactt gctcggcaac
     2041 ctgctctgcg cggtcgccgg tctgctcgac cccaataact cgctcgccaa cttcctggac
     2101 ggtctcctaa gggcactgta cccactgtag tcgggtattc gtcagtacgg gtctggaaga
     2161 gaggggcagg tttctcgctt gcccctctac tcatggtgct acatcgccgg agcgatagcg
     2221 gattttagaa gaacggagtc ggtagcctta ccagcaacgg cgctgcgcct gccgtaaccc
     2281 tcgttgtata actagagtcc atttagagtg cgggccggct tgcttcttga cgtgaaagca
     2341 gctgaagagc gacaaagcgc gttatagcta tgccgacccg ctcttgcatt gtacgtcaat
     2401 agcggcgagt ctggatcaca ccgctatccc cttcgccgga gtatcagcct tcggatcacg
     2461 gcgtcctaga agaacctctt gagagccccg accgcgccct gcttccatgg caccctgtgc
     2521 gagacgcccg aggcgttctc gaacttcttc aagttctcca ggtaccagcg gaagttccgg
     2581 atcagggcct ccttgttcga gaacttcggc gcgtagccga gctgcctctc ggccttctct
     2641 atggagacga aggagtcctt cgacgccgtc tcgtagaccc acttgtaaag gggggagagt
     2701 ttcagtttgt ctagaacgcg caggccccag atcgccggtg cggcggggaa gccgacgac
//
LOCUS       DATAIR010000605         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_318699, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000605 DATAIR010000000
VERSION     DATAIR010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318699"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VHM69_14390"
     CDS             <1..604
                     /locus_tag="VHM69_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX2741630.1"
                     /translation="TKEDIGLEGTNIFLGKHSGRHALKDALEELGYYVEGEVLKRAFT
                     RFKEIADHKKTVTAADLEAIAADEVGSFEGKFVLESFRVVAATGRQSRAAVTISHADH
                     GTFEAEAEGEGPVDAVFRAIDAATNIKGRLTDFRIDAVTGGKDALGEVRVSVEFEGGD
                     YAGRGLSQDVVEAAARAYVRAVNVYTAGMVGARWDAQVAP"
     gene            580..1350
                     /locus_tag="VHM69_14395"
     CDS             580..1350
                     /locus_tag="VHM69_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741631.1"
                     /translation="MGRAGSAVEAPERRGAAPPGYECLDGDVPVVHAPRLAEKAGEVQ
                     GYLAQGYRTLAEVLSVEPPEIAALLVADEDWDEAPRDNARPYPPGLPYFTRSVEPPAL
                     VLPEELSPVFRPCTEVTQPLTVWHEMAHAFLLRREVVRTPAWLREFVPQAAAAAVASK
                     VGLSLDEHVSRIDRDPGFTVRDVRGPAGADDQMSFQNLLLLLGTAALDEFGDGFLTKL
                     VHALWEENEIVDEVRAEELFADALGPGGREWLMSRPEF"
     gene            1358..2425
                     /gene="leuB"
                     /locus_tag="VHM69_14400"
     CDS             1358..2425
                     /gene="leuB"
                     /locus_tag="VHM69_14400"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX2741632.1"
                     /translation="MRSSFEILLLPGDGIGPEVVGATREVVDAAADHFGIEMRYEERR
                     IGGTAIREEGEPISDETLDAASASDSVLLGAVGHPDFDDAPVRPEAGLLKLRKHLGAF
                     ANLRPVAAIPSLIGSSPLKREVVEGVDVLIVRELTGGAYFGEKEEGEERASDLSVYTR
                     EEVVRVARVAFEAAKRRKRRVTSVDKANVMATSRLWRKVVTGVAKDYPDVELEHVLVD
                     AAAMYLIQSPKRFDVILTENLFGDILSDEAAILPGSMGMLPSASLGKPGSPGIFEPVH
                     GSAPDIAREGKANPYATILSAAMMFRHSLGRPDVAEAIEQGVSVALEAHFLTPDLGGK
                     KTTEEVTKAVSKWVAAGEGVA"
     gene            2428..>3003
                     /locus_tag="VHM69_14405"
     CDS             2428..>3003
                     /locus_tag="VHM69_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX2741633.1"
                     /translation="MKLFDTTLRDGTQREGVSMTAEDKARIARELDSLGIHYIEAGFP
                     ASNPKDREFFEGFDASELANAKLVAFTRARRPNGRAEDDPAVTLLPSLSAPVACIVAK
                     SWKLHVEKVLRTTPEENLESVRDTVAYLADAGKEVIFDAEHYFDGFRDDPAYSLAVLR
                     AAAEAGATTLVLCDTNGGTLPTELKAIVSRTV"
BASE COUNT          552 a          922 c         1029 g          500 t
ORIGIN      
        1 gaccaaagaa gacatcggcc tcgaggggac gaacatattc ctgggcaagc actcgggccg
       61 ccacgccctc aaggacgccc tcgaagagct cggctactac gtcgagggcg aggtgctcaa
      121 gcgggccttc acgcgcttca aggagatcgc agaccacaag aagacagtaa ccgccgcaga
      181 cctcgaagcc atagccgcag acgaggtcgg ctccttcgag ggcaagttcg tcctggagtc
      241 gttccgggta gtcgccgcaa cgggccgcca gagccgggcc gccgtcacca tctcgcacgc
      301 cgatcacggc acgttcgagg ccgaggcaga gggcgagggc ccagtggacg ctgtcttccg
      361 cgccatcgac gccgcgacga acatcaaggg ccgcctgacg gacttccgca tcgacgccgt
      421 gacgggtggt aaggacgccc tcggcgaggt gagggtctcc gtcgagttcg aagggggcga
      481 ttacgccgga cgcggcctct cccaggacgt cgtcgaggct gcggcgcgcg cctacgtgcg
      541 ggcggtcaac gtctacacgg ctggcatggt cggagccagg tgggacgcgc aggtagcgcc
      601 gtagaagccc cggagagacg gggagccgct ccgccaggct acgaatgcct ggacggcgac
      661 gttccggtag tccatgcccc aaggctggct gagaaagccg gggaggttca gggctatctc
      721 gctcagggct atcggacgct cgcggaggtg ctgtccgtcg agccgcccga gatagccgcg
      781 ctcctcgtgg cggatgaaga ctgggatgag gcgccgcgtg ataacgcgcg tccttaccca
      841 cccgggcttc cgtacttcac ccgttccgtc gagccgcctg ccttagtgtt gccggaagaa
      901 ctatctccgg tgttcaggcc atgcacggaa gtcacgcagc ccctgaccgt ctggcacgag
      961 atggcccacg ccttcctgct gcggcgggag gtcgtgagga caccggcatg gttgcgtgag
     1021 ttcgtcccgc aggcagcggc tgcggccgtc gccagcaagg tcggtctgtc actggatgag
     1081 cacgtgtcgc ggatagacag ggacccggga ttcaccgtgc gggatgttcg tggtcctgcc
     1141 ggggcggatg atcagatgtc gtttcagaat ttgctacttc tactcggcac ggcggcgctc
     1201 gacgaattcg gagacggatt tctgacaaaa ctcgtccacg cgctctggga agaaaatgag
     1261 atcgtcgatg aggtgcgggc cgaggagttg tttgctgacg ctctgggtcc tggcgggcgt
     1321 gagtggctga tgtcgaggcc ggagttctag gaggatcatg agaagctcgt tcgagatatt
     1381 gttgctccct ggcgacggca tcggtcccga ggtggtcggg gcgacgcgcg aggtcgtcga
     1441 cgcggctgcg gatcacttcg gtatagagat gcgctacgag gagcgcagga tcggcggcac
     1501 ggccatccgc gaggagggcg aacccatctc cgatgagacg ctcgacgccg ccagcgcctc
     1561 cgactcggtc ctgctcggcg ccgtgggcca cccagacttc gacgacgctc ccgtacgccc
     1621 cgaggcaggt ctcttgaagc tcaggaaaca tctcggggct ttcgccaatc tcaggcccgt
     1681 ggccgccatt ccgtcgctca tcggctcctc acctctcaag cgcgaggtcg tggagggcgt
     1741 tgatgtgctc atcgtcaggg aattgacagg tggcgcgtac ttcggagaga aggaagaggg
     1801 tgaggagagg gcgagtgatc tcagcgtgta cacacgcgag gaggtcgtgc gggtggcgcg
     1861 ggtagcgttc gaagcggcga agcggcgcaa gcggcgcgta accagcgtgg acaaggcgaa
     1921 cgtgatggcg acgagccggc tgtggcggaa agtcgtcacc ggggtggcta aagactatcc
     1981 cgatgtggag ctcgaacacg tcctcgtgga tgccgctgcg atgtacctga tccaatcccc
     2041 aaagcgcttc gacgtgatac tgaccgagaa cctcttcggc gacatcctct cggacgaggc
     2101 ggcgattctc ccaggctcga tgggcatgtt gccctccgca tctctgggca aaccgggctc
     2161 gcccggcatc ttcgagcctg tacacggctc agcccctgac atagctagag aggggaaggc
     2221 gaacccctac gcgaccatcc tctccgccgc aatgatgttc cgccattctc tgggccgccc
     2281 ggacgtggcc gaggccatcg agcagggcgt ctccgtcgcc ctggaggcgc actttttgac
     2341 gcctgacctt ggtggaaaaa agacgacaga agaggtaacc aaggccgtga gcaagtgggt
     2401 cgcggcggga gagggtgtgg catgaccatg aagcttttcg atacgacgct acgcgacggg
     2461 acccaacgcg aaggcgtaag catgaccgcc gaggacaagg cgcgcatagc ccgcgagctg
     2521 gactctctgg ggatccacta catagaagcg ggcttccccg cctccaaccc gaaggaccgg
     2581 gagttcttcg agggcttcga cgcctcggag cttgcgaacg cgaagctggt cgcctttacc
     2641 cgtgcgcggc gcccgaacgg gcgcgcggag gacgaccccg cggtaacgct cctcccctcc
     2701 ctctccgcgc ccgtcgcctg catcgtcgcc aagagctgga agttgcacgt cgagaaggtt
     2761 ctcaggacca cgccagagga gaacctggag tccgtcaggg ataccgtggc gtatctcgca
     2821 gacgcgggca aagaggtcat cttcgacgcg gagcactact tcgacggctt ccgcgacgac
     2881 cctgcgtatt cgcttgcggt gctgcgcgct gcggctgagg caggtgcgac gacgctggtc
     2941 ctctgcgaca cgaacggtgg gacgctgcct acggagctga aggcgatcgt ctcgcgcacg
     3001 gtg
//
LOCUS       DATAIR010000606         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_411203, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000606 DATAIR010000000
VERSION     DATAIR010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411203"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VHM69_14410"
     CDS             complement(<1..810)
                     /locus_tag="VHM69_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX2741634.1"
                     /translation="MNAYPIKLTAHVRAYAFGERLIPEILAKEDVPEGTVAETWEISD
                     YRETTGKVKNGPYTGRTLHDLVEEFPDELVGEGWSGPYFPLLEKFIDASHMLPVHLHA
                     DDETARTKHGEANGKTEAWHIIRAAEGASILAGVREGVGRDELREAFLAQDHDSVMPR
                     YPIRAGDTVYVPGGVIHAFGPDTLVFEVQQTSDLGQFVTPMSLYGERLDDETWRSNIE
                     AALDELRPDYRPRPNPGLVLQDDGNRRVVCCAGPHFALERWTLTEPLTEPSH"
     gene            949..1827
                     /locus_tag="VHM69_14415"
     CDS             949..1827
                     /locus_tag="VHM69_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741635.1"
                     /translation="MTLYETDDARTEPRMKVLLTGATGYIAGQLLPAFRERYDLRLVD
                     VKDVDASGNRVEGVEVSDLLSDDDSTLEPLFSDMDVVVHCGHFKPDGDDPRSLYQGER
                     RNVDMMQRVYQLALEGGARRVVAASTNQAAKWYERPYYAGLRDRVGPEDYPRPDSFYG
                     WAKAAYEPLGFLYACGSLGRKLEVVQIRIVVPREIDAALFEDEPPERYVRELAGYISE
                     RDMQQLFCKSAETPDIDDQHGVPFHIFYGVSDNARKFWSITNARKVIGYEPRDDSEIR
                     FANDIARLLHREEPGA"
     gene            1824..2249
                     /locus_tag="VHM69_14420"
     CDS             1824..2249
                     /locus_tag="VHM69_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2741636.1"
                     /translation="MITDLGHPAFAAHDVDETISFYGMLGIEEAFRLHNDDGSLMLAY
                     LHVSGDRFLEVFPGGPPPDPDRTQSFMHICLLTEDVHAAVEHLRENGAPIDREPVVGL
                     DGNWQAWTRDPDGNEIELMQLSEESPQWRTARSAERSET"
     gene            2267..>2775
                     /locus_tag="VHM69_14425"
     CDS             2267..>2775
                     /locus_tag="VHM69_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741637.1"
                     /translation="MLDHARLKDFAPGEGLQFRAFDERFEEEGWLPVPVPGDVHRALL
                     DAGRIDDPYYDRNEGDCAWMEDREWWYRMTFDGPEDPLLPDERLRLVFHGLDTFATIW
                     LNGEELGKHHNMFREAVFDVGDRIRPGENTLAILFDRPLDHVGEEHPDQWGRNPERVF
                     MRKAQFGFG"
BASE COUNT          524 a          896 c          863 g          492 t
ORIGIN      
        1 gtgggacggc tctgttaagg gctcggtgag ggtccagcgt tcgagggcga agtgcgggcc
       61 ggcgcagcag actacgcgtc ggttcccatc gtcctggagg acgaggccgg ggttcgggcg
      121 cggccggtag tcagggcgga gttcgtcgag ggcggcctcg atgttcgacc tccaggtctc
      181 gtcgtccagg cgctcgccgt agaggctcat gggcgtcacg aactggccga ggtccgaagt
      241 ctgctggacc tcgaagacga gcgtatccgg gccgaaggcg tggatcacac ctcctggcac
      301 gtacaccgta tcgcccgccc ttatcgggta gcgcggcatg acggagtcgt gatcctgcgc
      361 cagaaaagcc tcgcgaagct cgtccctgcc gacaccttct ctgacgccgg caaggatcga
      421 ggccccctcc gcagcccgga ttatgtgcca cgcttcggtc tttccgtttg cctcgccgtg
      481 ctttgtgcgc gccgtctcgt catcagcgtg caggtggacg gggagcatgt gcgaggcgtc
      541 gatgaacttc tcgagcaacg ggaagtacgg cccgctccag ccctccccca ccagttcgtc
      601 agggaactcc tctacgaggt cgtggagagt ccttcctgtg taaggtccgt tctttacctt
      661 gcccgtcgtc tccctgtagt cgctgatctc ccaggtctca gccaccgttc cttctggaac
      721 gtcctccttg gcgagtatct cggggatcag acgttccccg aaagcgtatg ccctgacgtg
      781 cgcggtgagc ttgatcggat acgcgttcat cgtctctccc cggttcgtcc ttctcccaac
      841 acggacattc tacagtggtg acgagacgtg aacccgtaaa cggctcgtgc gcgaagcccg
      901 cgattgtgag tagatattgc agacgagctt cgactcagga ggctactctt gaccctttac
      961 gagacggacg atgcacgaac agagccgcgc atgaaggtcc tgctgacggg cgcgacgggc
     1021 tacatcgcag gccagctcct ccctgcgttc agggaacgct acgacctcag gctcgtcgat
     1081 gtcaaggacg tagacgcctc gggtaacagg gtcgaagggg tcgaggtctc cgacctgctc
     1141 tcggacgacg actcgactct tgagcccctc ttctctgaca tggacgtggt ggtccactgc
     1201 gggcacttca agcctgatgg cgacgaccct cgctcgctct accaaggcga gcggcgcaac
     1261 gtggacatga tgcagcgcgt ctaccagctc gcgctcgaag ggggcgcgcg gcgggttgtc
     1321 gcggcaagca ccaaccaggc agcgaagtgg tacgagcggc cctattacgc agggctacga
     1381 gaccgcgtcg gtcccgaaga ctatccgcgg cctgacagct tctacggctg ggccaaggcc
     1441 gcctacgagc cgcttggatt tctgtatgcg tgcggcagcc tcggacggaa gctggaggtc
     1501 gtccagatcc ggatagtcgt gccgcgtgag atcgacgcgg cgctcttcga ggacgaaccg
     1561 ccggaacgct acgtccgcga gctagcaggc tatatcagcg agcgggacat gcaacagctc
     1621 ttctgcaagt ccgccgagac gccggatata gacgaccagc acggcgtacc cttccacatc
     1681 ttctacggcg tctcggacaa tgccaggaag ttctggagca tcaccaacgc acgaaaggtc
     1741 atcggctacg aaccacggga cgactccgaa atccgcttcg ccaacgacat cgcccgcctg
     1801 ctgcacagag aggagcccgg cgcatgatca cagacctcgg acaccccgcc ttcgccgcac
     1861 acgacgtaga tgagaccatc agcttctacg ggatgctcgg catcgaagag gctttcaggc
     1921 tgcacaacga cgacggctcg ctcatgctcg cgtacctgca cgtctcgggc gacaggtttc
     1981 tcgaggtctt ccccggcggg ccgccccccg accctgacag gacgcagagc ttcatgcaca
     2041 tctgcctgct gaccgaagac gtacacgcgg ctgtcgagca cctgcgcgag aacggcgcgc
     2101 caatagacag ggagcctgtc gtcggcctcg acggtaactg gcaggcctgg acgcgcgacc
     2161 ccgacggaaa cgagatcgaa ctcatgcaac tctccgagga gtcgccgcaa tggcggacgg
     2221 cccgttcggc ggagcgtagc gagacgtaga gagcgggggg aactccatgt tggatcacgc
     2281 gagactcaaa gactttgctc caggtgaggg gctccagttc cgtgccttcg acgaacgctt
     2341 cgaggaggag ggctggctcc ccgtccccgt ccccggagac gtccaccggg ctttgctcga
     2401 cgcaggacgc atcgacgacc cttactacga ccgcaacgaa ggcgattgtg cctggatgga
     2461 agaccgcgag tggtggtaca ggatgacctt cgacggtccc gaagatccac tcctgcccga
     2521 tgagcggctg cgcctcgtct tccacggtct cgacaccttc gccaccatct ggctgaacgg
     2581 cgaagagttg ggcaagcacc acaacatgtt ccgagaagcc gtcttcgacg tcggagatcg
     2641 gatacgcccc ggggagaaca ccctcgccat cctcttcgac cgtccgctcg accacgtcgg
     2701 agaagagcac cccgaccagt ggggaagaaa ccccgagcgc gtcttcatgc gcaaagccca
     2761 gttcgggttc ggatg
//
LOCUS       DATAIR010000607         6516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_203537, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000607 DATAIR010000000
VERSION     DATAIR010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203537"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1159
                     /locus_tag="VHM69_14430"
     CDS             92..1159
                     /locus_tag="VHM69_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX2741638.1"
                     /translation="MAKGRTVLPLMVLVLAAVLLGVPSVRSALPGMTHRATYTNPVIR
                     WDFADPTVLKAGDGWYYAYSTENLTTERLAYIQAARSRDLVKWELLPDAMPEKPDWAS
                     TTRDFWAPGVIEADGRYYMYFSGVHDERGSMCLGVATSGSPGGPFVPEDEPLRCGDGF
                     ENIDPMPFDDPRTGESLLYWGSDGAPIRVQRLAPDRLHFARGSNAIALVYTDIIQPYE
                     RLVEGAFVVYRDGFYYLFYSGDNCCSAPASYGVMVARSRSATGPFEKRSETTGEWGAN
                     VILNMNERWEGPGHNSVIRDEAGQDWIFYHAIDPEDRYNPGTEAPKRPMLMDPIVYRS
                     GWPAIEGQSPSTSERQGPLVP"
     gene            complement(1168..2409)
                     /locus_tag="VHM69_14435"
     CDS             complement(1168..2409)
                     /locus_tag="VHM69_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX2741639.1"
                     /translation="MRSEDRVAEATGGRYPLVEDRIQAEIMHHVRAMDRPVSRSEITE
                     VLGVSRSKISLEVGCLIGAGLLVEDGLAKSEGGRRSSLLRIPRAAGLIAAVDLGATST
                     DVALTTLGGELLVHRGEPGEVKDGPRRVLGRVKELLSELLAEQRADAKDVLAIGVGVP
                     GPVEHASGVLRSPPIMPGWDGFPICSVFAGEYDAPVFVDNDVNVMALGEHRDGVGKGE
                     DNVLFVKIGTGIGAGIIADGHLQRGSQGCAGDIGHICVDPEGPVCSCGNRGCLEAMAA
                     APAIAAKAERCAREGLSPVLEEMFDERGALTARDVGEAASLGDYHALQIIRGSGKLVG
                     QVLATLVSTLNPSIIVVGGGVANIGNSLLAEIRSTVYRRSLPLATRNLPIVLSKLDQQ
                     AGVTGASVLAAEGVLQTSMAG"
     gene            complement(2602..3972)
                     /locus_tag="VHM69_14440"
     CDS             complement(2602..3972)
                     /locus_tag="VHM69_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX2741640.1"
                     /translation="MRERFRDVRGFAVPLDYTYRIKLPHRPGSLARVTGTIAEGEGLI
                     GDVNTISVGRTTSVREITIEVRDQDHAAHIAEMLDTLDGVKVLWSRDRALLRHVGGKL
                     IIESTRPVRTVQDMRDVYTPGVARACVAISEEPALAGELTMIGRSVAICTNGSRVLGL
                     GDIGPVASMPVMEGKAVFYHQLANVSAMPILIDTKDVDEFVETVVRIAPTFGGIHLED
                     ISAPECFEIESRLIEALDKPVMHDDVHGTAVVTLAAVLVACRHAGLKLEEEVVGQIGL
                     GAAGFGIAALIADAGARRVLGSDPNPLSHERAEKKGIEIADMETVMREADIVIATTGR
                     PGLIDPGMVREGQVILALTNPYPEIDAEAAVEAGAAFAADGTSVNNVLGYPGIFRGAL
                     LSGAEEINLPMKLAAAEKIASLASGSELVPDALDSSVHKQVADTVRDAAIESGIAHLD
                     KAPMGL"
     gene            complement(4039..4437)
                     /locus_tag="VHM69_14445"
     CDS             complement(4039..4437)
                     /locus_tag="VHM69_14445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2741641.1"
                     /translation="MSRRVITSSKLPAPMRGGAFSAGVEAPAGRTVYVSGQVSMDAEG
                     NVVGEGDIQRQTETVLEHVKTVVEEAGGGMDDIVKVTVFITEMGLYDEIHEVRRRYFE
                     EPYPASSMVEVSALIDPRLLIEIEAVAVVG"
     gene            4544..5776
                     /locus_tag="VHM69_14450"
     CDS             4544..5776
                     /locus_tag="VHM69_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX2741642.1"
                     /translation="MQRTFDVQSVREQFPALRRKYDGRGVVYFDGPGGSQVARQAIDA
                     MSGYMERGGANLHGVFPTSTETEEILSETRLAAADFLGAGPEEISFGANMTTLTFAIS
                     RALAREWDEGSEIVVTELDHRANVDPWLIAASERGAKVRWVKVDPETLILDQDDLEQN
                     INERTKLVAVGLASNAVGTVSDVAAVAERAHSVGGVVAVDAVHAAPHIPIDRDAIGAD
                     VITCSAYKFFGPHVGVSAIKRDLFEQMDVYRLDPAPDQIPDKLETGTQNHEGIAGVKG
                     ALDFISSLGEGTSRRQRLASAMRTVEEHETDLAETFRAALRDLPKVKLYAAPEGVRKT
                     PTIAFRVEGHAPEEICERMLEHGFFIAAGDFYASTLAQKLGIKDSGGFVRAGLAPYNT
                     EEEVERFVKVLDRLVRKG"
     gene            5973..6176
                     /locus_tag="VHM69_14455"
     CDS             5973..6176
                     /locus_tag="VHM69_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2741643.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHHTGIAGEGFKTLDE
                     GAKVTYEATQGRKGMQAENVTPA"
     gene            6292..>6516
                     /locus_tag="VHM69_14460"
     CDS             6292..>6516
                     /locus_tag="VHM69_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741644.1"
                     /translation="MQYIIAVLVGLALAEVATLITTIYLHRVLSHKAIRLHPAVTMFM
                     RFGTWMLTSISPREWVAVHRKHHNFSDVEGD"
BASE COUNT         1225 a         2101 c         2009 g         1181 t
ORIGIN      
        1 tacgtcgtga ggggagagac gctggtgata ctctccggcc taccgagaga agatcttatc
       61 cggatggcaa gctcgctgag gcccgtagag aatggctaag gggcgcaccg tcttgccgct
      121 gatggtcctc gtgctcgctg ccgttttact gggcgtgcca tcggtgcggt ctgcgttgcc
      181 gggaatgacc catcgcgcga cgtacacgaa ccctgtcata cgatgggact tcgctgatcc
      241 gaccgtgctc aaagctggcg acggctggta ctatgcgtac tcgaccgaaa acctgactac
      301 cgagcgcctg gcgtacatcc aggccgcgcg ctccagggac ctggtgaaat gggagttact
      361 ccccgacgcc atgccggaga agcctgattg ggccagcacg acccgtgact tctgggctcc
      421 gggtgtcatc gaggctgacg gtcgatacta catgtacttt tcgggcgtcc acgacgagcg
      481 aggtagcatg tgcctcgggg tcgcaacgtc aggttctccc ggcgggccgt tcgtccccga
      541 ggacgagccc ttgcggtgcg gcgacggctt cgagaacata gacccaatgc ctttcgacga
      601 cccgcgcacg ggagagtcgt tgctgtactg gggatctgac ggtgcgccga tccgcgtcca
      661 acggctcgcc ccggaccggc tccatttcgc cagggggtcc aacgccatag cgctcgtcta
      721 cacggacatc atacaaccct acgagaggct ggttgagggc gcgttcgtcg tctaccggga
      781 tggcttctac tacctcttct actccggtga caactgttgc tcggcgccgg cgagctacgg
      841 cgtgatggtc gcccgttcac gcagcgccac gggtccgttc gagaagcggt cggagacgac
      901 cggcgagtgg ggcgccaacg tcattttgaa catgaacgag cgatgggaag gcccgggaca
      961 caactcggtc atcagagacg aagcggggca ggattggatc ttctaccacg ccatagatcc
     1021 ggaggaccgc tacaaccctg gcaccgaggc gcccaaacgt ccaatgctca tggacccgat
     1081 cgtctaccgc tcaggctggc cggcgatcga aggacaatcg ccctccacga gcgaacggca
     1141 ggggccactc gtcccctgat aggatctcta tccggccatt gacgtctgca gcacaccttc
     1201 agcggcgaga acgctggccc ctgtgacgcc ggcctgctgg tcgagcttgc taagaactat
     1261 ggggaggttg cgggtagcga gcggcagcga ccgacggtag acggtgctcc ggatctcggc
     1321 cagcagcgag ttgcctatgt ttgccacacc tcccccgacc acgattatgg aagggttgag
     1381 cgtgctgacg agggtggcaa gcacctgtcc cacgagcttt cccgaccccc ttatgatctg
     1441 gagcgcatgg taatccccga gggaggccgc ttcgccgacg tccctcgcag tgagcgcgcc
     1501 acgctcgtcg aacatttcct ccaggacggg tgagaggccc tccctcgcac agcgctctgc
     1561 tttggcggcg atggctggag ctgcggccat cgcttccagg catcccctgt tgccgcacga
     1621 gcagacaggc ccttccgggt ccacgcagat gtgccctatg tcccccgcgc acccctggct
     1681 gcctcgttgc aggtgcccgt ccgctatgat gccggcgccg atgccggtgc cgatcttgac
     1741 gaagagcacg ttgtcctctc ctttgccaac gccgtccctg tgctcgccca gcgccatgac
     1801 gttgacgtcg ttgtccacga agacaggggc gtcgtactcg ccggcgaaca ccgagcagat
     1861 agggaacccg tcccaaccag gcatgatcgg cgggcttctc aaaactcccg atgcgtgctc
     1921 cacgggaccg ggaaccccca cccctatggc aagtacgtcc ttcgcatccg ccctctgctc
     1981 ggcgaggagc tccgaaagca gctccttcac cctcccgagc actctccgtg gcccgtcctt
     2041 gacctcccca ggctctcccc tgtgtaccag aagctcaccg cccagtgtgg tcagggcgac
     2101 gtccgtggaa gtggccccga ggtccacggc ggcgatcagg ccagccgcac gggggatgcg
     2161 caagagcgag gaacgacggc ccccctcgga cttggccaga ccatcctcaa cgaggaggcc
     2221 ggccccgatc aagcatccca cctcgaggga gatcttgctc ctgctcacgc caagaacttc
     2281 cgttatctca gaacgcgaca ccggacggtc catcgccctg acatgatgca tgatctccgc
     2341 ctgaatgcga tcctcgacaa gcggatagcg accaccagtt gcctctgcta cccgatcttc
     2401 gctcctcaca tgtacagtat agagaaactt gttccacaat gccaacgact tttgccgcct
     2461 cttgtcataa gtcgcaatga gcaggcacga gtgttatttg gccaagaagg tcgatggagc
     2521 ggaaccctcg tgccgggcat gatctatcgg gtgaggggtg cttatgccgt ggaggttcta
     2581 cataacacga ggcttattcc ttcaaagacc cattggcgcc ttgtcgaggt gagcgatgcc
     2641 gctctctatt gcggcgtctc tgacggtgtc ggcgacctgc ttgtgcacgc tagagtcgag
     2701 ggcgtcgggg acgagttcgg agcctgaggc gagggaggct atcttctctg ccgcggcgag
     2761 cttcatggga aggttgatct cctcggcccc cgaaaggagc gccccgcgga agatgccagg
     2821 gtagccgagc acgttgttca cgctcgtccc gtccgcggcg aaggccgccc cagcttcgac
     2881 ggcggcttct gcatcgatct ccggataggg gttggtgagg gcaagtatta cctgaccttc
     2941 acgcaccatg ccagggtcta tgaggccggg tctccccgtc gtcgcaatga cgatgtctgc
     3001 ctcgcgcatg acggtctcca tgtctgcaat ctctatgccc ttcttctcgg cgcgctcgtg
     3061 ggagaggggg ttcgggtccg agccgagcac gcgccttgcg cccgcatcgg cgatgagggc
     3121 ggcgatcccg aatcccgcgg cgccgagccc gatctggccg accacctcct cttccaactt
     3181 cagcccggcg tggcggcagg cgacgagcac ggccgccagc gtcacgaccg cggtgccgtg
     3241 gacgtcgtcg tgcattacgg gcttgtcgag ggcctctatg aggcgggact ctatctcgaa
     3301 gcactccggc gcggagatgt cctccaggtg gatcccgccg aaagtcgggg cgatgcgcac
     3361 gacggtctcg acgaactcgt ccacgtcttt cgtgtcgatc aggatcggca tcgccgagac
     3421 gttggcgagc tggtggtaga acacggcttt gccttccatc acgggcatcg aggcgacggg
     3481 accaatgtcg ccaaggccga ggacccgcga gccattcgtg cagatggcga cgctgcgccc
     3541 gatcatggtg agctcgcctg cgagcgcggg ctcctcggag atggcgacgc aagcccgcgc
     3601 caccccaggg gtgtagacgt cgcgcatgtc ctgaaccgtc ctcacggggc gggtcgactc
     3661 gatgatgagc ttcccgccca cgtgccgtag cagcgccctg tcccgcgacc acaggacttt
     3721 gacgccgtcg agcgtatcca gcatctctgc gatgtgcgcg gcgtggtcct ggtcgcgtac
     3781 ctctatcgtg atctcacgca ccgaggtggt ccgcccgacg cttatcgtgt tcacgtcgcc
     3841 gatcagaccc tccccctcgg ctatcgtccc cgtcaccctg gctagcgagc ccggacggtg
     3901 cggcaacttg atgcggtacg tgtagtcgag cggcacggcg aaccccctga cgtcccggaa
     3961 acgctctctc aacatcccgc cacagtgcgg gcgctggata cctgattata taggtgaccg
     4021 tccctccggc gctgaagctt aacccacgac cgcgacggct tcgatctcga tgaggagcct
     4081 cgggtctatg agggctgaga cctcgaccat ggatgacgcc ggatacggct cctcgaagta
     4141 ccttctcctg acctcgtgga tctcatcgta gagccccatt tccgtgatga agaccgtcac
     4201 tttgactatg tcgtccatcc cgccgccggc ctcctcgacc acggtcttta catgctcaag
     4261 caccgtctcg gtctgacgct gtatgtctcc ctcgccgacc acgttccctt cggcgtccat
     4321 cgagacctgc cccgagacgt agaccgtgcg ccccgccgga gcctcgaccc cggcggagaa
     4381 cgcgccccca cgcatcggcg ccggcaactt cgagctggtg atcacacgcc tcgacataac
     4441 gacctcctca cctatgttcc actcccgaga gtacagtagc atccgggccg cgcgcctgat
     4501 gtaaactcgc caaccaggag atcttcgagg cgggagagga cttatgcagc gtacgttcga
     4561 cgtgcaatcc gtccgcgaac agttcccggc tttacgccgg aagtacgacg ggcgcggcgt
     4621 cgtgtacttc gatgggcccg gcggctcgca ggtggcgcgg caggccatag acgccatgtc
     4681 gggctacatg gagcgtggag gcgccaacct ccacggcgtc ttcccgacga gcaccgagac
     4741 agaggagatc ctgtccgaaa cccgcctggc ggcggcggac ttccttggag ccggaccaga
     4801 ggagatctcc ttcggcgcca acatgacgac cctgaccttc gccatctccc gcgccctcgc
     4861 gcgcgaatgg gacgaaggct cagagatagt tgtcacggag ctcgaccacc gcgccaatgt
     4921 tgacccctgg ctcatcgccg cgtcggagag aggggcgaaa gtgcgctggg taaaggtcga
     4981 cccggagacc cttatcctcg atcaggatga cttagaacag aacatcaacg agaggacgaa
     5041 gctcgtggcc gtcggcctgg cctcgaacgc cgtcggcacc gtgagcgacg tggccgccgt
     5101 cgccgaaagg gcccacagtg tcggtggcgt cgtcgccgtg gacgccgtgc acgcggctcc
     5161 gcacatcccc atagaccgcg atgctatcgg ggccgacgtc atcacgtgct ccgcctacaa
     5221 gttcttcggc ccgcacgttg gggtgtcggc aattaagcgg gatctcttcg aacaaatgga
     5281 tgtctacagg ctggaccccg cgccggacca aatcccggac aaactggaga cgggtaccca
     5341 gaaccatgag ggaatagccg gtgtcaaagg cgcccttgat tttatctcct ccctcggcga
     5401 aggaacctca cggcgccagc ggctcgcgag tgccatgcgg accgtcgaag aacacgaaac
     5461 agatctcgcc gaaaccttcc gcgccgcgct acgggatctg cccaaagtaa agctctacgc
     5521 cgcccccgaa ggcgtccgca agaccccgac catcgccttc agggtcgagg gacacgcgcc
     5581 ggaagagatc tgtgagcgga tgctggagca cggcttcttc atcgccgcag gagatttcta
     5641 cgcctccacg ctcgcgcaga agctcggaat aaaggacagc ggcgggttcg ttcgagccgg
     5701 cctcgccccg tacaacacag aagaagaggt tgagaggttc gtcaaggtac tggacagact
     5761 ggtgcgcaag ggatgatctc taccggcggt cggtgagcag gcggcctctt gatgacaaaa
     5821 tatcctggcg agcgacccgc aggcagagtt ctgctatact acgtgccggt aacgtaaggt
     5881 gggtttacag tagacggagc gccacccccg tgagatagag ggtggttttt tgtttagtgg
     5941 ggcccaggaa tacgaaaggt gggctccagt atatggccca aggaacagtg aagtggttca
     6001 gcgacgagaa gggctacggc ttcatctccc cggacgatgg aggtgaggac ctcttcgtgc
     6061 accacacagg gatagcaggt gaaggcttca agaccctcga cgagggcgcc aaggtaactt
     6121 acgaggcgac ccaggggcga aagggcatgc aggcggagaa cgtgactccg gcttaacacc
     6181 cgagaagcaa aagtaagcgc aaaggccggg gtataaaccc cggcttttgt cgtgtaagca
     6241 ggattatcag aacgcgctgg gcgaggcctg gacccgttcg agggaggtct cgtgcaatac
     6301 ataatagctg tgctcgtggg tttggcgctc gctgaggttg cgaccctgat caccacgatc
     6361 tatctgcaca gggtgctgtc gcacaaggcg atccggctcc atcctgccgt gacgatgttc
     6421 atgcgcttcg ggacgtggat gctcacgagc atctcgccgc gcgagtgggt cgccgtccac
     6481 cgcaagcacc acaacttcag cgacgtcgaa ggagac
//
LOCUS       DATAIR010000608         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_134576, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000608 DATAIR010000000
VERSION     DATAIR010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134576"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VHM69_14465"
     CDS             <1..516
                     /locus_tag="VHM69_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741645.1"
                     /translation="DGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEDE
                     RREIMPVAARHTIPELMEAARYFVTRTRRKLFFEYTLLAGVNDQPRHVEALAELLDHP
                     LYHLNLLRFNWTDTGFSATSKRDAREFLRYARELGLSATLRPSRGQDIDAACGQLAAK
                     DLRGRPAVPMV"
     gene            513..992
                     /locus_tag="VHM69_14470"
     CDS             513..992
                     /locus_tag="VHM69_14470"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2741646.1"
                     /translation="MKDEPAERLDPRAKTLWRITGVLNMLPLLVGAGFASWALPRFGG
                     VSFFVGVLPVLAVLTLAVFVVGVAPGLRWRRWRYEIRLDEVDLQRGILWITRTLVPLA
                     RIQHVDTRQGPLQRRFGLSTVIFYTAAGPNQIPELSTPVAAQARDRIAALTRDQDEI"
     gene            982..2433
                     /locus_tag="VHM69_14475"
     CDS             982..2433
                     /locus_tag="VHM69_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2741647.1"
                     /translation="MRSEEHLHPVAMLIGAIKTTRRWISALVFPGIAFLVSQGFSLWT
                     MTLFLFGLIVVAMLAALWGFLSWRATTYTVTGNAFRLRQGVFQKNERTIPLDHVQSVD
                     TVQGIIQRVFGVVEVRIETAGGGANEPDASLSALDLEAAEALRREIEGSQRERSEDAA
                     GPTVLRRLSRRELLIAGATSGQIGIALSLLAVGSQFLDEFFSEDFVRGLVETFAPNWL
                     MVVLIVLPAGTLLAWLLAIAGTVLAFSGFTLSREGDFLYIKRGLLERREATIPLARIQ
                     AVRISEGVLRQPFGLAALRVESAGYGEDAGVSTTLFPLLPRHEVDEFLSVAAPEFAVD
                     PALKPLPRRALRRYVFRATVPALIAAVVSAGLLFLAFESALGLLALLLVPPAAAYGWL
                     GYRDAGWAFEEDRLVVRYRSLGRKTAVAPRRRLQSRGMVRSPLQRRLRLATFLAEVAS
                     GSGGSTLRVTDLDERSAESLVGNLGPGARLAEA"
     gene            2649..>3003
                     /locus_tag="VHM69_14480"
     CDS             2649..>3003
                     /locus_tag="VHM69_14480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX2741648.1"
                     /translation="MRRLVGLGAVGLWHQGVARYLVAIAFAIVLGLSAAVPVFAADID
                     GQYQPSIVGGDPVPNGKYPFMVSLQADTSGASPSKEHFCGATLIDSDSVLTAAHCADY
                     IGAATIPGISVSYRDV"
BASE COUNT          461 a          969 c          991 g          582 t
ORIGIN      
        1 gacgggttcg ggttggcggc gaggcacatc gcggtctcga cgagtgggtt ggtggacaag
       61 atccggcgtt tcgcggacga gcccgagcag ttccaccttg cgatatcgtt gcataccccc
      121 ttcgaggacg agcggcgcga gatcatgccg gtcgcggccc gccacaccat ccccgagctg
      181 atggaagcgg cgcgttactt cgtgacacgg acgcgccgca agctcttctt cgagtacacc
      241 ctgctcgcgg gggtcaacga ccagccgcgt cacgtcgagg ccctggctga actcctcgac
      301 catcccctct accacctgaa cctgctccga ttcaactgga cggacacggg gttttcagcc
      361 acctccaagc gcgacgccag ggagttcctg cggtacgcga gggagctcgg cctttcggcg
      421 acgctgaggc cgagccgggg acaggacata gacgccgcat gcggccagct cgccgcgaaa
      481 gaccttcgcg ggcgtcccgc cgttcccatg gtttgaagga cgaacccgca gagaggctcg
      541 atccgcgggc gaagactctg tggaggatca cgggcgttct gaacatgctg ccgctccttg
      601 ttggggcggg tttcgcgagt tgggcgctgc cgcggttcgg tggcgtgtcc ttcttcgtgg
      661 gggttctgcc cgtactcgcg gtcctcacgc tcgccgtgtt cgtggtcggg gtggcgccgg
      721 ggttgcggtg gaggaggtgg cggtacgaga tccggctcga cgaggttgac ctgcagcgtg
      781 ggatcttgtg gatcacccgt acgctggtgc cgctggcccg catacagcac gtggacacca
      841 ggcaggggcc cttgcaacgc cgtttcgggc tctccaccgt cattttctac accgcggcag
      901 ggccgaacca gatcccggag ctctcgaccc ccgtcgccgc gcaggcgcgg gaccgcatcg
      961 ccgcgctgac cagggatcag gatgagatct gaggagcacc tccaccccgt cgcgatgctc
     1021 atcggggcta taaagacgac ccggcgctgg atcagcgcgc tcgtcttccc agggatagct
     1081 ttcctcgtga gccaggggtt cagcctgtgg accatgacgc tcttcctgtt tggcctcatc
     1141 gtcgtggcca tgctggcggc gctctggggt ttcctttcgt ggcgggcgac gacctacacg
     1201 gtcactggca acgctttccg gctgcgccag ggcgtcttcc agaaaaacga gcggacgata
     1261 cccctggatc acgtacagtc cgtggatacg gtgcagggga tcatccagcg cgtcttcggc
     1321 gtcgtcgagg tgcgcataga gacggcgggc ggcggcgcga acgaacctga cgcttcgctc
     1381 tcggcgctgg acctggaagc ggcggaggcg ctgaggcgtg agatcgaagg ttcccaaagg
     1441 gagaggtcgg aggatgctgc ggggccaacc gtcctcaggc gtctctcgcg gcgggagttg
     1501 ctgatagccg gggccacgag cggccagatc gggatcgccc tctccctgct cgccgtcggg
     1561 tcccagttcc tcgacgagtt tttctccgag gacttcgtcc ggggtctcgt cgagaccttc
     1621 gcccctaact ggctgatggt tgtcctgatc gtcttgcctg ccggaacgct acttgcctgg
     1681 ctccttgcta tcgcagggac cgtcctcgcc ttctctggct tcacgctctc ccgcgaggga
     1741 gacttcctgt acatcaagcg cgggctcctg gaacgtcgcg aggcgacgat tccgctcgcc
     1801 cgcatacagg ccgtcaggat ctcagagggc gttctgcgcc agcctttcgg tctcgccgcc
     1861 cttcgcgtcg agagcgcagg ctacggcgag gacgcgggtg tctcgaccac gctcttccca
     1921 ctccttcccc gccacgaggt ggatgagttc ctgtccgtcg cagcccccga gttcgccgtt
     1981 gatccagcac tgaagccgct accgcgccgc gccctccggc gctacgtctt ccgggctaca
     2041 gtccccgccc tgatcgccgc cgtggtttct gcaggtctct tgtttctcgc cttcgaatcg
     2101 gctctgggac tactcgcgct gcttctggtg ccaccggccg ccgcttacgg ctggctcggt
     2161 tatagggacg ccggatgggc gttcgaggag gaccgcctgg tggtgcgcta caggagcctg
     2221 ggccgcaaga cagccgtcgc gccccggcga cggctgcagt cgcgaggcat ggtcaggagc
     2281 ccgctccagc ggcgtctacg gcttgcgacc ttcctggccg aggtcgcctc cggctccggc
     2341 ggatccaccc ttcgggtcac ggacctcgac gaaaggtccg ccgagtcact ggtggggaac
     2401 ctggggccgg gagcccgtct cgcggaagcc tgaggaccgt ggtaggttag gcgaacctca
     2461 cggaacctgg cgccctccgg cgtgaggtac ggtctctcaa gtacgtctga cggagcgtgg
     2521 ctgtgagcat tgaagatccg gcacctatga ggcgcgcggg agccggaaaa acagtacttc
     2581 ctagcactac ttcatcggct acgcgtacta tattatcgcc ctggcgatac gagcggggga
     2641 acatacgcat gagaagactt gttgggttgg gcgcggtagg tttgtggcac cagggcgtcg
     2701 cacgttacct ggtggcgata gccttcgcga tagtgctggg tctgtcggct gcggtgccgg
     2761 tattcgcggc ggacatagac ggtcagtatc agcccagcat agtcggagga gatcctgtcc
     2821 cgaatggtaa gtatcctttt atggtctcct tgcaggcgga tacgagcggc gcttctccca
     2881 gcaaggaaca cttctgcggt gccaccctca tcgactccga cagcgtgttg acggctgcac
     2941 actgcgccga ctacataggc gccgctacca ttcccggtat ctccgtgtca taccgcgacg
     3001 tgc
//
LOCUS       DATAIR010000609         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_456801, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000609 DATAIR010000000
VERSION     DATAIR010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456801"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..234)
                     /locus_tag="VHM69_14485"
     tRNA            complement(163..234)
                     /locus_tag="VHM69_14485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Thr,seq:tgt)
     gene            complement(412..900)
                     /locus_tag="VHM69_14490"
     CDS             complement(412..900)
                     /locus_tag="VHM69_14490"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX2741649.1"
                     /translation="MALILDGYNVIGALDRYREPESLEVARELLINDALKAAGWTGQQ
                     LVVVFDAHRGPEPERTELRAGGAVRVIYSSPGESADDVIERLLSTLESPVTVYTADFA
                     LQRTALARGSTRATPREFANLLDELPAVTRSPDTPRKSRVADRLSLETLRRLEEIRRR
                     GT"
     gene            complement(891..1601)
                     /gene="rlmB"
                     /locus_tag="VHM69_14495"
     CDS             complement(891..1601)
                     /gene="rlmB"
                     /locus_tag="VHM69_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX2741650.1"
                     /translation="MKQETIYGIRPVVEALRSGRRRVLEVLDAVGDREVAMAAGARGV
                     VVKKFSRQRIEELTRGAVHQGVAARVEPYPYSGLEEILSTPEPLVLVLDGVTDPRNLG
                     AVLRAADGAGASGVVVPKDRAVGVTAAAVKASAGASEHMRVARETNLKRAIDRMKQAG
                     LWVYAAGTGGTPYTELDLAGPVALVLGSEGRGVRRLVREACDGEVSIPMLGAVESLNV
                     SVAAAVLLYEARRLRGWP"
     gene            complement(1602..>2849)
                     /gene="cysS"
                     /locus_tag="VHM69_14500"
     CDS             complement(1602..>2849)
                     /gene="cysS"
                     /locus_tag="VHM69_14500"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2741651.1"
                     /translation="FVQNITDIDDKIINKANDEGVSWQEIVRRYTDSYHERLEMLGIG
                     LPDVEPLATEHIPEMISLIEELIEKGHAYAPSEANGDVYYAVESFPDYGALSHQRPEE
                     MRETERGRTGFKRSPLDFTLWKGSKPGEPSWESPWGPGRPGWHIECSAMVSKHLPDGA
                     DVHGGGTDIRFPHHENELAQSAAAHPDHPFVRAWAHHGMVTLASGKMAKSVGNVLDVQ
                     RAVDIHGRNAVRMWLLQSHYSQPIEYSEEILEEKRRSYVRLSRLHRQIEKATSSSEMS
                     DQIAAWLRERFDTAMRDDLNTPEAIAALFEAARQAGHEISVYPETAETFASLAEAIEE
                     IMTIFGFDFAEEFSSTLSGVQVWHEETPDERPNDAIVRRVVERERARLAKDWLLADRL
                     RDELYAEGWLVADTPRGPFVSRR"
BASE COUNT          488 a          962 c          839 g          560 t
ORIGIN      
        1 aggattcgaa cctccgtagg cgaagccggc agatttacag tctgctccct ttggccgctc
       61 gggcacaccc ccggccacgc ccccgtggca ctctgcggag cgcccggata tggtagcaaa
      121 cgccaccgac ttttcaacag acgcgggcga gcccgcaacg agagcccggg aagggattcg
      181 aacccccgac cggccgctta caaggcggct gctctacctc tgagctaccc gggcgaatag
      241 gcttcaggtt taggcctcag gtctcaggct tcaggtcaga tcactgcgcc tcgaagcctg
      301 tacccctctg ccagcactat tctaccaagg caagcatccg gacgggggcg tcgttagcga
      361 aaacacgttc cgcttacccc ttcctgatgc ctgtaacctg atacctgaag cctatgtccc
      421 cctgcggcgt atctcttcaa gcctacgcaa agtctccaac gaaagtcggt ctgctacacg
      481 ggacttgcgc ggggtgtcgg ggctgcgggt cacggcgggt agctcgtcga gcaggttcgc
      541 gaactcgcgc ggggtggcgc gggtcgagcc gcgggcgagg gcggtgcgtt gcagggcgaa
      601 gtctgccgta tagaccgtga ctgggctctc gagggtggag aggaggcgtt cgatcacatc
      661 gtccgcagac tctccggggg agctgtagat cactcgcaca gccccgccgg cccgtaactc
      721 ggtcctctcc ggctccggac cgcgatgtgc atcgaagacg acgaccaatt gttgacccgt
      781 ccaccctgcc gccttcaggg cgtcgtttat cagtagctcg cgcgcgactt cgaggctctc
      841 cggctcccta tagcggtcga gagccccgat gacgttgtag ccgtcaagga tcagggccac
      901 ccgcgcagcc tccgagcttc gtagagcaag accgccgcag cgacggagac gttgagcgac
      961 tctacagcgc ctagcatcgg gatggagacc tcgccgtcgc acgcctcgcg cacgagccgc
     1021 cgcacgcctc tgccctcgct cccgagcacc agcgccacag gtcccgcgag gtcgagttcc
     1081 gtatacggcg tcccgccggt cccggcggcg tagacccaga gacctgcttg cttcatcctg
     1141 tcgatggcac gcttgaggtt ggtctcccgc gcgacacgca tgtgctcgct cgcaccggcg
     1201 ctcgccttga ccgcggccgc cgtcaccccc accgccctgt ccttcgggac cacgaccccg
     1261 ctcgcgcccg caccgtcggc ggcccgcagg acagctccca ggttgcgcgg gtcggtgacg
     1321 ccgtcgagaa ccagcaccag aggctcaggg gtggacagga tctcctccag cccggaatag
     1381 ggatacggct cgacgcgtgc cgcgacgccc tggtgaaccg ctcccctcgt cagctcctct
     1441 atccgctgcc gggagaactt cttcacgact acgccgcgag cgccggcggc catcgcgacc
     1501 tccctgtctc ctacggcgtc aaggacctcc aagacccttc gacgtccgct cctgagcgcc
     1561 tcgaccacgg gcctgatgcc atagatggtc tcctgtttca attagcggcg gcttacgaag
     1621 ggacctctgg gcgtatcggc aactagccaa ccctccgcgt acaactcatc acgcaaccgg
     1681 tcagccagaa gccagtcctt tgcgagtcgc gcccgttctc tttcaaccac tcgacgcacg
     1741 atagcatcat taggtctttc gtcgggtgtc tcttcatgcc agacttgaac tccgctcaag
     1801 gtacttgaga actcctccgc gaaatcgaag ccaaagattg tcatgatctc ttcgatggcc
     1861 tctgcgagcg acgcaaacgt ctccgcggtt tcaggataga ctgaaatctc gtgtcccgcc
     1921 tggcgagccg cttcgaagag cgcggcgata gcttcaggcg tgtttagatc gtctcgcatc
     1981 gcggtgtcga aacgttcgcg tagccaagca gcgatttggt cagacatctc ggaggagctg
     2041 gttgcttttt caatctgtcg atggaggcgc gaaagcctta cataggaacg tcgcttctcc
     2101 tcaaggatct cctctgagta ctctatgggc tgcgagtagt ggctctgcaa cagccacatg
     2161 cgcacggcgt tcctgccatg gatgtccacg gctctttgca cgtcgagcac gttcccgaca
     2221 gacttcgcca tcttgccgct cgccagcgtc accatcccat gatgcgccca ggcgcggacg
     2281 aacggatggt caggatgggc cgcggcgctc tgcgccaatt cgttctcgtg gtgcgggaag
     2341 cgtatgtccg tgccgccgcc gtggacatcc gcgccgtccg gcaggtgctt gctcaccatc
     2401 gcagagcact cgatgtgcca gccagggcgc cctggtcccc agggactctc ccaggaaggc
     2461 tcgcccggct ttgacccttt ccaaagcgtg aagtcgagcg gactcctctt gaagccagtc
     2521 cggcccctct ccgtctcccg catctcttcg ggacgctggt ggctcaaggc gccgtagtca
     2581 gggaagctct ctacggcgta gtagacgtcg ccgtttgctt cgctcggcgc gtaggcgtgg
     2641 cccttctcta tcagctcctc gatcaaggag atcatctctg gaatgtgctc tgtggccagc
     2701 ggttcgacgt cgggcaaacc tatgccgagc atctcaagcc gttcgtggta ggagtcggtg
     2761 tagcggcgta cgatctcctg ccacgagacg ccctcgtcgt tggccttgtt gatgatcttg
     2821 tcgtctatgt ccgttatgtt ctggacgaa
//
LOCUS       DATAIR010000610         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_65099, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000610 DATAIR010000000
VERSION     DATAIR010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65099"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VHM69_14505"
     CDS             complement(<1..797)
                     /locus_tag="VHM69_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2741652.1"
                     /translation="MAANPVSKNATSAYDASSIQVLEGLEAVKRRPGMYIGSTGPRGL
                     HHLVREIIDNSIDEALAGYCTEILVTIHTDNSVTVTDDGRGMPVGTMEKYGKPAAEVI
                     MTTLHAGGKFGGDGYKVSGGLHGVGASVVNALSEWLELDIRRDGHVWSQRYERGFPKS
                     DLTKDRKLKKGEGTGTIVTFMPDPDVFTETQDFSFDTLSRWFREWAFLNKGLKIRLVD
                     ERGEDRDVTYQYEGGIRDFVEHINESKDPVHRSVFYLEREEENGDVEV"
     gene            complement(957..>1547)
                     /locus_tag="VHM69_14510"
     CDS             complement(957..>1547)
                     /locus_tag="VHM69_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741653.1"
                     /translation="FSSERTLATWDRKVVELGTIVLEGREAALPILEEYFKVSLRALY
                     GPEKATIRYGHSATLQEYSEALREAHSADVERGTTSIGPHRDDFEVLLGGVNLTIFGS
                     QGQQRLATLALKFAARDYVRGAVGEDPILLFDDVMSELDERRREYLAKYFLESTQAVI
                     STTNLEYFGEEILRRTRIIRISGGSISETATDGAGK"
BASE COUNT          265 a          507 c          429 g          346 t
ORIGIN      
        1 acctctacgt cgccgttttc ttcctcgcgc tccaggtaga aaacggacct gtggacgggg
       61 tccttcgact cgttgatgtg ctccacgaag tccctgatcc cgccctcgta ctggtacgtg
      121 acgtccctat cctccccgcg ctcgtcaacg agccggatct tcaaaccctt gttcaggaaa
      181 gcccactcgc ggaaccagcg gctcaaggtg tcgaaagaga aatcctgggt ctctgtgaag
      241 acgtccgggt caggcatgaa ggttacaatc gtccccgtcc cctcgccctt cttgagcttg
      301 cggtccttcg tgagatcgct cttcgggaag ccgcgctcgt agcgctggct ccaaacgtgg
      361 ccgtcgcgcc tgatgtccag ctccagccac tcggagaggg cgttcaccac ggaggcgccg
      421 acgccgtgca atccaccgga aaccttgtag ccatccccac cgaacttccc gccggcgtga
      481 agggtcgtca tgatgacctc tgcggcgggc ttgccatact tctccatcgt acctaccggc
      541 atgccgcgtc catcatctgt gaccgtgacg gagttgtccg tgtggatggt gacgagtatc
      601 tccgtgcagt acccggcgag cgcctcgtct atcgagttgt ctatgatctc ccggacgagg
      661 tggtggagtc ccctcgggcc cgtcgagccg atatacatgc cgggacgccg cttgacggcc
      721 tcgagtcctt cgaggacctg gatcgaactt gcatcgtacg cgctcgtagc attcttggat
      781 acagggttag ccgccaaaga accggaccct cctcgtaatt agaccggcgc cttcgccgga
      841 gttccgttct tcttatcgtc acattgcacc tgtagattgt aacacatccc ccgcgctttc
      901 ccaacggccg ttgtagccgt tttgtggctt atataagcct gtttcgcagg gccgcgctac
      961 ttccctgcac catctgttgc ggtctctgaa atggaaccac cagatatgcg tatgatacgt
     1021 gtccttctaa ggatttcctc gccaaaatat tcgaggttcg tcgtcgagat gacggcctgg
     1081 gtactctcga ggaagtactt tgcaagatac tctctgcggc gctcgtccag ctcgctcatc
     1141 acgtcgtcga agagcaggat cgggtcctcc cccaccgccc cccgtacgta gtccctcgcg
     1201 gcgaacttga gtgccagcgt cgcgagccgc tgctgccctt gcgacccgaa gatggtcagg
     1261 ttgaccccgc cgagcaagac ttcgaagtcg tccctgtgcg gccctatgga ggtcgtcccc
     1321 cgctcgacgt cggcgctgtg cgcctccctg agcgcctcgg agtactcttg gagggttgcg
     1381 ctatgcccgt agcggatcgt ggccttctcc gggccgtaca gggcccttag agagactttg
     1441 aaatactctt cgaggatggg cagcgccgcc tcgcggccct ccaggactat cgttccgagc
     1501 tcgacgacct tcctatccca ggtagccagc gtcctctcgg aggagaa
//
LOCUS       DATAIR010000611         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_249848, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000611 DATAIR010000000
VERSION     DATAIR010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249848"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1140
                     /locus_tag="VHM69_14515"
     CDS             247..1140
                     /locus_tag="VHM69_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2741654.1"
                     /translation="MPKGRSLGARLSERSLGASPTVLVMFSIVSAQLGAAIAKDLFDS
                     LGPGGTVFLRISFAALVLFLLVRPKLRGHDRSGYVVATVFGLALAGMNLSLYLALDRI
                     PLGVAVTLEFIGPLGVAVAGSRRMLDLLWVVLAAAGILLLAPLGVLGGTDLDPVGVAF
                     ALLAGCLWASYILLSARTGSAFPGGTGLVIALCIGTLVLLPVGIAGAGYALLDPRLLL
                     AGFGVAMLSSAIPFFLDLEALRRLPARVFGVLMSLEPAVAALAGFVVLGERLELRAVA
                     AILLVTVAAAGASRFGRHEVG"
     gene            1313..2416
                     /locus_tag="VHM69_14520"
     CDS             1313..2416
                     /locus_tag="VHM69_14520"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2741655.1"
                     /translation="MRTPERTLILRELNRATLARQMLLDRETLTVLNAVERLVGLQAQ
                     VVSPPYVGLWTRLRDFRRDELTRLMQERRVVRATLMRATLHLMTAEDYLLLRPALQPA
                     LTRSMKTTTGRRVDGLNVERLVGEARAYFEEEPRTFADLRPLLSELEPERDLSALAYA
                     VRTHLPLVQVPSGGAWGYSGKAPFTTVEQWLGRPLSGSEDPRELVLRYLAAFGPATVR
                     DVQTWSGRIGLKQVIEELKPELRTFSDERGNELLDLPDAPLPDPDTFAPPRFVPDYDN
                     LVLSHADRRRVISDEHRKKVFLSAARVRATFLIDGFVSGTWKVEKTKRTATLVIEPFE
                     SLSVKVRDALQDEGERLVHFVGEGAEAFEVRFG"
     gene            complement(2493..>3525)
                     /locus_tag="VHM69_14525"
     CDS             complement(2493..>3525)
                     /locus_tag="VHM69_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2741656.1"
                     /translation="PHEAAISAGRCPTVAIGGLVLGGGIGFSSRKLGLTCDSLRETEI
                     VTADGKILTCNEKKNEDLFWACRGGSGGNFGVNVSYTFQSTPVKDVSIYDLQWDLEDG
                     EKVLPALQDVVLQAPDDFSCRMGGGRSGKDGGGRSPANVNALGQYFGPREELLEILEP
                     VLRLAKPTKRVIEDKTFWQAKDYFFHNTPTDHFQVKSSFVQKTLPEQGIEELLRYVNS
                     WPGSSNEDGAGFAMFALGGAISRIPEEKTAYAHRDSRFLLAIDSSWASSDSHRTEQEN
                     VDWVEGFADGMRPYTSEYAYQNFIDRSQPDWARAYYGDNLDRLVRIKRLRDPDDFFHF
                     RQSVPQRLS"
BASE COUNT          584 a         1115 c         1128 g          698 t
ORIGIN      
        1 gtcgaacatc tccctgtccg cgccgcagtc gggacacttc tccggtagtt cgccttcgac
       61 ccggtagccg cagttggtgc aggcccagtt gaggggttct gcttcaccct gcacgccgtg
      121 cgtctcgtcg gtcatccgtg atcctcctcg tattgtgaga gatcattatg tcatcggcga
      181 agaccgggtt cacgctgatg ctagactatc cgcgccgtaa ccgcgaccgg aaaggttgtc
      241 actacgttgc cgaaaggtcg ctcgctcggc gcccgcctct cggaacgatc actgggcgcg
      301 tcgccgacgg tcctcgtgat gttctcgata gtctcagccc agctcggggc cgccatagcc
      361 aaagacctct tcgattctct gggccccggc ggtacggtgt tcttgaggat ctcgttcgcc
      421 gcgctcgtgc tgttcctcct tgtgcgcccg aagctccggg ggcacgaccg gtcgggatac
      481 gtcgtcgcca ccgtcttcgg gctcgcgctg gcggggatga acctttcgct ctacctcgca
      541 ttagaccgca tacccctggg cgtcgccgtg acgctggagt tcatcggtcc gctcggcgtt
      601 gctgtcgccg gctcccggcg aatgttggac ctgctatggg tcgtgctcgc cgccgcaggc
      661 atcctcctgc tcgctcccct cggggtgctc ggtgggacgg acctcgatcc cgtaggcgtt
      721 gcattcgcgc ttctggcagg gtgtctctgg gcctcatata tcctcctcag tgctcgtacg
      781 gggagcgcgt ttcctggtgg gacggggctt gtgatcgcgc tctgcatagg gacgctcgta
      841 ctcttgcctg tcgggatagc gggcgccggc tacgccctgc tcgatccgag gctccttctc
      901 gctggcttcg gcgttgcgat gctctcctcg gcgatccctt tctttctgga tctcgaagcc
      961 ctgcgcaggc tccccgcccg tgtcttcggc gtcctgatga gcctggagcc cgccgttgcc
     1021 gcgctcgccg gcttcgtcgt tctaggcgaa cggctcgagc tgcgagccgt ggccgccata
     1081 ctcctcgtga ccgtcgcagc cgcaggtgcc tcgcggttcg gcagacacga agtgggctga
     1141 caggaacggg gtggtgaggt cttccgccac ccgtagaatg agaactccag agaagggccg
     1201 gggagcccgt tcgaggagcg aaaggagtat ccgtggccgc tgagggacga gacatttcgg
     1261 gggtggcgcc ggtcaccggc ggcttgttca gcgacaggga gcccctgccc tggtgaggac
     1321 gccggagcgc accctgatac tccgggagct caaccgggcg accctcgcac gccagatgct
     1381 actcgacagg gagacactga ccgtgctgaa cgccgtagag cgcctggtcg gccttcaggc
     1441 gcaggtggtg agccctccct acgtcggcct gtggacccgc ctgcgggact tccggcgcga
     1501 cgagctcacg cggctcatgc aggagcgacg ggtagtcagg gcgacgctca tgcgcgcgac
     1561 gttgcacctc atgacggcgg aggattacct tctgctgcgt cccgccttgc agcccgcgct
     1621 cacccgctcc atgaaaacca ccacgggcag gcgggtagat ggattgaacg tagagaggct
     1681 cgtcggggag gcgagagcgt acttcgagga agagccgcgt accttcgccg acctgcgtcc
     1741 cctgctctct gagctggaac cggagaggga cctctcagcc ctcgcctatg ctgtgaggac
     1801 ccacctcccg ctcgtacagg tgccatctgg tggcgcgtgg ggctactcgg ggaaggcacc
     1861 gttcacgacc gtcgagcagt ggctcggcag accgttatca ggttcggagg acccgcgcga
     1921 gctcgtactc cggtacctgg cggcgttcgg tcccgcgacg gtgcgggacg tccagacgtg
     1981 gtcgggtagg ataggcctga agcaagtcat cgaagagctc aagccggagt tgcgcacctt
     2041 cagcgacgag cgtggtaacg aactgctcga cctccctgat gcgcctttgc ccgaccccga
     2101 caccttcgcc ccgccgcgct tcgtaccaga ttacgacaac ctggtgctct ctcatgccga
     2161 caggaggcgt gtgatctcgg acgaacaccg caagaaggtc ttcctctcgg ccgccagagt
     2221 cagggccact ttcctcatcg acggcttcgt gagtggaacg tggaaggtcg agaagacaaa
     2281 gagaacagct accctcgtga tcgaaccctt cgagtctcta tccgtgaaag tccgtgacgc
     2341 ccttcaagat gaaggcgagc ggctcgtcca cttcgtcgga gagggcgcgg aggcgttcga
     2401 ggtcaggttc gggtagcgag tgaaatcgtg gagccgagac gaggaggcac ggtttcagat
     2461 ccaaacccgg cgaaaagacg tggcgaccgg gctcaggaga gacgctgcgg cacgctctga
     2521 cgaaaatgga agaagtcgtc ggggtcgcga agacgcttga tgcgaaccag gcgatccagg
     2581 ttgtcaccgt agtaggcacg tgcccagtca ggttgtgaac ggtcgatgaa gttctggtag
     2641 gcgtactccg acgtgtatgg tcgcatcccg tcggcgaacc cttcgaccca atcgacgttc
     2701 tcctgctcgg tgcggtgcga atcgctgctt gcccaggagc tgtcgatggc caggaggaac
     2761 cgcgagtcgc gatgcgcgta ggcggtcttc tcttcgggga tgcgtgagat ggcaccgccg
     2821 agggcgaaca tggcaaagcc tgcgccgtcc tcgttcgagc ttcccggcca actgttaacg
     2881 taccggagta gctcttcgat cccctgctcg ggaagtgtct tctggacgaa ggacgacttg
     2941 acctggaagt gatcggttgg ggtgttgtgg aagaaatagt ccttggcctg ccagaaggtc
     3001 ttgtcttcga tgaccctctt cgttggcttc gccaggcgca gtaccggctc caatatctcg
     3061 agaagttcct cgcgcggtcc gaagtactgg ccgagtgcgt tgacgttcgc cggacttcga
     3121 ccgccgccgt ctttccccga acggccgccg cccattcggc aggagaagtc atcgggagct
     3181 tgcaggacga catcctgtag cgcagggagc accttctctc cgtcttcgag atcccattgg
     3241 agatcgtaga tcgacacgtc ctttaccgga gtcgactgga aagtatagga gacgttgacg
     3301 ccgaagttgc ctccgctgcc tccccggcag gcccagaaca gatcctcgtt cttcttctcg
     3361 ttgcaggtga gtatcttccc gtcagccgtg acgatctcgg tctccctcaa gctatcgcag
     3421 gtcagtccga gcttgcggga actgaagccg atcccaccac ccagaaccag accgccgatc
     3481 gcgaccgtcg gacaacgccc cgcggagatg gccgcctcgt gcggc
//
LOCUS       DATAIR010000612         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_411633, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000612 DATAIR010000000
VERSION     DATAIR010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411633"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VHM69_14530"
     CDS             complement(<1..460)
                     /locus_tag="VHM69_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX2741657.1"
                     /translation="MIRRCTLVSVGKLKGWAAEGSEDYAKRLRRHFQVNIVEVAEEDM
                     NRRSREEVLAAEGGRILKRLPADVYVISLDSEKGKQHTSEGLARRLEDLGLSGRSNVA
                     FVIGGPLGLSTEVLQRADELWSFGKITLPHALARVVLLEQLYRAVKIQRGE"
     gene            complement(457..1281)
                     /locus_tag="VHM69_14535"
     CDS             complement(457..1281)
                     /locus_tag="VHM69_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2741658.1"
                     /translation="MRLSVLSSGSSGNATYMETDSGGLLVDAGLSRRRIEALLARIGR
                     NLADVGAVLITHGHADHTCGVRSLVREYGIEVFAAPGVGVSFRATAVSPGESCRVGDL
                     TATFFEVPHDSLTYGVRVADHESTVAMATDLGEAGVEVLRRMRGAEAVVLEANHDHDW
                     LRRGPYSSSLKRRISSRRGHLSNRQAAEAAVVLAPHGLKDLVLAHLSKTNNSPTRACG
                     TVHGALRESGYAGVRVRAAIAGQPTPWIEVGAPLEVVEHVYHYGESDAAGQLFGIE"
     gene            complement(1337..>2070)
                     /locus_tag="VHM69_14540"
     CDS             complement(1337..>2070)
                     /locus_tag="VHM69_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2741659.1"
                     /translation="ATGVADGNQPESAETTLVSRDHEDETRPENDGSEDRDSGKTVEA
                     VLPEDVLSEKQLEHQEVPKYEYWHKLSDSQPKRLVSTDSSVGTIPAVKPFNLGRDPGG
                     PGDRTLYLTVPKLGLKRIPVFDEVSEEKLKESVVHVPVTGFPWQEGANTYIAGHRIGY
                     PGTGSSYVFFHLDWLMEGDEILLRDAAGKDYQYRVTEKVKVSPDNVEVMEPKVGESLV
                     SLQTCTLPDYKKRLIVHGELVRTSA"
BASE COUNT          326 a          679 c          646 g          419 t
ORIGIN      
        1 tctcgcctcg ctgtatcttt acggcgcggt agagctgttc gaggaggacg accctggcga
       61 gggcgtgagg gagtgtgatc tttccgaagg accagagttc gtccgctctt tgcaacacct
      121 cagtggagag gccaagcgga cctccgatca cgaaggctac gttgctacgg cccgagaggc
      181 cgaggtcttc gagcctccgg gcgagtcctt ccgaggtgtg ctgcttgccc ttctcgctgt
      241 cgagggagat cacgtacacg tcggcgggaa ggcgctttaa gatacgccct ccttccgcgg
      301 cgagcacctc ctcgcgggag cggcggttca tgtcctcttc tgcgacctcg acgatgttta
      361 cctggaaatg gcggcgcagg cgcttcgcgt agtcctcgga gccctcggcg gcccatccct
      421 tcagttttcc gacagagacg agcgtgcagc gccggatcac tcgataccga agagttgtcc
      481 cgcggcgtca ctctcgccgt agtggtagac gtgttcgacc acttccagcg gcgcgccgac
      541 ctcgatccag ggcgtcggct gccccgctat ggccgcgcgt actctgacgc cggcgtaccc
      601 cgactccctg agcgccccgt gtacggttcc gcaggctctg gtcggggagt tattggtttt
      661 cgataggtgg gcgagtacaa ggtctttgag cccgtgggga gccagcacca cagcggcctc
      721 ggcggcctgc cggttcgata gatggccgcg cctggaggag atgcgccgct tcaggctcga
      781 cgagtatggg ccgcgcctga gccaatcgtg gtcgtggttc gcctccagga ccaccgcctc
      841 ggcaccgcgc atgcgccgca gtacctcaac gccggcctcc ccgaggtccg tcgccatcgc
      901 cacggtgctc tcgtggtccg cgacccttac cccgtaggtc aatgagtcgt gggggacctc
      961 gaagaaggtc gctgtgagat ctccgaccct gcaggactct ccgggagaca ccgcggtcgc
     1021 cctgaatgac acgccgacgc cgggggcggc gaacacttcg atgccgtact cgcgcaccag
     1081 cgagcgcacg ccgcaggtgt ggtcggcgtg tccgtgagtg atcaggactg cccccacgtc
     1141 ggccaggttc ctgccgatac gggcaaggag ggcctctatg cgtcgccgtg agagaccggc
     1201 gtccacgagg agtccgccgg agtccgtctc catataggtc gcattgcccg agctgccgct
     1261 cgaaaggaca ctcagccgca cagcagtcct tctggttcag tcctcgctcg cacgttgaga
     1321 tcttagcacg ctgcgctcag gcgctcgtcc ggaccagctc tccgtggacg atgaggcgtt
     1381 tcttgtaatc gggcagagtg catgtctgca gggacacgag cgattcgcca accttcggct
     1441 ccatgacctc cacgttgtcc gggcttacct tcaccttctc cgtcacgcgg tattggtagt
     1501 ccttgcccgc ggcgtcccgg aggaggatct catccccctc cattagccag tcgaggtgga
     1561 aaaagacgta tgatgaaccc gtgccggggt acccgatgcg gtgacccgca atgtacgtgt
     1621 tcgccccctc ctgccacggg aaccccgtga ccgggacgtg gacgaccgac tccttgagct
     1681 tctcctccga aacttcgtcg aagactggaa tgcgcttcag ccccagcttc gggaccgtca
     1741 ggtagagcgt tctgtcgcca ggtccgccag gatcccgtcc caggttgaac ggcttgacgg
     1801 ccggtatggt gcctacggag gagtcggtgg aaaccagcct tttcggctgg gaatccgaca
     1861 gtttgtgcca gtactcgtac ttcggcactt cctgatgctc tagctgcttt tccgagagta
     1921 cgtcctcggg gagcaccgct tcgaccgtct tcccggaatc gcggtcttcc gagccgtcgt
     1981 tttccggtct ggtttcatcc tcatgatccc tggaaacgag tgtcgtctcc gcgctctctg
     2041 gctggttccc atcagcgacg cccgtggcgg
//
LOCUS       DATAIR010000613         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_249907, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000613 DATAIR010000000
VERSION     DATAIR010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249907"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1386)
                     /locus_tag="VHM69_14545"
     CDS             complement(58..1386)
                     /locus_tag="VHM69_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741660.1"
                     /translation="MERLGGEQEGRTSSIRQVALASFIGTAIEWYDFFLYGTAAALIF
                     GELFFPEFSSTAGTLAAFSTYAVGFAARPLGGIVFGHYGDRIGRKTMLVLSLMIMGIA
                     TALIGFLPTYQSIGIWAPIILVVLRFFQGIGVGGEWGGAVLMAVEHAPEGRRGFYGSW
                     PQMGVPAGLLTGNLAFLAATAWLPEAWGWRVPFLFSMVLVGVGLFIRLRIMESPAFTQ
                     VKESGTEAPMPILDVLRTYPKNVLLAMGMRVAENGTFYVLTVFVLTYIVEELKMEQTT
                     GLVGVLIAATIGLFTVPFFGHFSDRVGRRPLYLLGSGFSLLFAFPFFWLLNTGVEPLV
                     WLAIILGVNIGHDAMYGPQAAFFSELFGTRVRYSGASLGYQLASVLAGGFAPLISLYL
                     LTTFGYGAVALYMAGMALITVISVLLATETYQGDITETRAEERRFIAEGP"
     gene            complement(1492..2955)
                     /locus_tag="VHM69_14550"
     CDS             complement(1492..2955)
                     /locus_tag="VHM69_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX2741661.1"
                     /translation="MTGVSWTNPRMGACVALLSGAVLALLVVAATVSRTADAGAVEDS
                     GSNSRHCHKLPRQVPHLVRNAEVQDGDCLKDLTTAGTEDTGHTDKNDWSGLNAEGTNN
                     PSGIGGFQVDGYFHDGRTTPTPNTNNGWLHDSQFVIRFPNHWNGKLVITGAPGVREQY
                     ANDFIISDWVLAKGYAFASTDKGNTGVSFYDDGSAPGGSVLEWHRRVEQLTRATKDAA
                     KRYYGEAPRRTYITGISNGGYLTRYALENTPELYDGGVDWEGTLWRAEGPNLFTYLPT
                     ALANYPEYATGDQQAHDNIIDAGFEPGSEFLWDEHYVTYWDLTQRIYREEFDPEYDGA
                     LKAGIPECQDEANIPGCDANYVYEDRPQSVRDAVRKVSLTGDIGKPLLTLHGDLDALL
                     PIRTDSDVYSELVRDKGHGNIYRYYVIGKGNHVDSYYDNNKDRLRPILPCHRDAFKAL
                     VGFIQRGIRPPESGYVPKPATGDVVNQCSIEATAPRP"
     gene            complement(3088..3687)
                     /locus_tag="VHM69_14555"
     CDS             complement(3088..3687)
                     /locus_tag="VHM69_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX2741662.1"
                     /translation="MKRVEFYYDLVSPYSYLAYGRIVRICEVNRAELALRPMLLGAVH
                     KAVGLQAPIEIKPKASYQARDIRRWAEYYGLPLQFPEPFPFRTLKTMRAAMFLRERGK
                     LEAFTREAFALYWEEGGAPEGREEADEDGPISSVARRVGAEPEEVLEGAASQETKQAL
                     KAATSEAVERGIFGAPAFFVNGEMFWGNDRLHFVEVALK"
     gene            complement(3751..5163)
                     /locus_tag="VHM69_14560"
     CDS             complement(3751..5163)
                     /locus_tag="VHM69_14560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741663.1"
                     /translation="MKAFDGNKGKGRRWSALGLLCVAQFVVVLDVTIVAIALPEIQDE
                     LGFSQAGLQWVISAYTLVFGGFLLLGGRAADLWGRRRLFVIGLLVFSGASLACGLADS
                     ALSLVVARVVQGLGAAIVAPSALSLLTTAFPEGEGRGRALGVWTAAAAGGGATGWLLG
                     GLLSGGLGWEWVFFVNVPVGVIGVALAPFFISESRDVSASPRLDLAGAVSVTVGLTLL
                     VYGITRVQEVGLGAPETLVSLGVSVVFLAGFVAVEGRVKYPLVPLGIFRSRAFLGANL
                     VALTLTASTTPPMLLCILYVQRVLGYSPAEAGLVFPPFNLAVIGGSLLGPRLVAWLGP
                     RAVMAFGLLAIAAGTLCFLGISRHGGYLYYLIPGFVLMGSGLGCASVASTASGTSAAD
                     GGLQGLASGLLNSAAQIGTVLGLAVLVPLSAARAEALAGTMPAEAALVEGFRSAFFGA
                     AGIAAVGIVVSLLIVRRKGA"
     gene            5220..5846
                     /locus_tag="VHM69_14565"
     CDS             5220..5846
                     /locus_tag="VHM69_14565"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX2741664.1"
                     /translation="METPRHIDELRIVGGHLALDFVNTVEGEPDGEPGFEFLRGYVDV
                     VAWSERVGVLSKEEAGRLALEAKRRPEEAEAVYRDALELRDTLYGVFRAVAEGGGPTS
                     GSLEVLGGYECEALSRGKLAPGDHGFRWEWTDGGDLASILWRVAHAATELLTSGSLDR
                     LKRCAHCRWVFLDESRNHSRRWCTMEVCGTHEKVRRYVAKRAARRSTM"
     gene            complement(5938..>6246)
                     /locus_tag="VHM69_14570"
     CDS             complement(5938..>6246)
                     /locus_tag="VHM69_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2741665.1"
                     /translation="FLFSLTFAEIPWQVVGALLVGGVIAAPLAAFIVKKLPARILGTV
                     VGGVILIVNMQTFFGAIGLQGAAANVGYFLIVTVWLAAIAFAIVALRRDRAVTESQPA
                     "
BASE COUNT         1266 a         2073 c         1870 g         1037 t
ORIGIN      
        1 gggcgccaca ggcagccctc tctcgtttct cacgtcttca gtgccttccg cctgaaacta
       61 tgggccctcg gcgataaacc ggcgctcctc ggcccgggtc tcggttatgt cgccctggta
      121 cgtctccgtc gccaggagca cagagatcac ggtgataagc gccatccccg ccatgtacag
      181 ggcgacggcc ccgtacccga aagtggtaag gaggtagagg gagatcaggg gcgcgaaccc
      241 tcccgcgagg accgaggcga gctggtagcc caaagacgcg cccgagtagc gaacccgcgt
      301 cccgaagagc tcagagaaga acgcagcctg gggaccgtac atcgcatcgt ggccgatgtt
      361 caccccgagg atgatggcca gccagaccag gggctcgaca ccggtgttca agagccagaa
      421 gaacgggaag gcgaacagca gcgagaaccc cgacccgagc aggtagagtg ggcgacgccc
      481 gacgcgatcc gagaagtgtc cgaaaaacgg gacggtgaag agcccgatag tcgcggcgat
      541 gagcacgccg acgagccccg tcgtctgctc catcttcaac tcctctacga tgtaggtgag
      601 cacgaagacg gtcaggacgt agaaggtccc gttctcggct acgcgcatcc ccatcgcgag
      661 caggacgttc ttcgggtacg tgcgcaacac gtccagaatc ggcatgggag cctcggtacc
      721 tgactctttg acctgcgtga aagccgggga ctccatgatc ctcagccgga taaagagacc
      781 gacgccgacc agaaccatgc tgaacaggaa aggcacccgc cagccccagg cctcgggaag
      841 ccacgccgtg gccgcgagga atgccaggtt ccccgtcaag agcccagccg ggactcccat
      901 ctgcggccag ctaccgtaga agccgcgccg accctccggc gcgtgctcga ctgccatcag
      961 gacggcgccg ccccactcgc cgcccactcc aatgccctgg aagaagcgca acacgacgag
     1021 tattatcggt gcccagatac cgatggattg gtaggtgggt agaaacccga tcagggccgt
     1081 tgcaataccc atgatcatta gagataggac gagcatggtc ttgcgcccta tcctgtcccc
     1141 gtagtggccg aagactatgc cgcccagcgg cctggccgcg aatcctacag cgtaagtgga
     1201 gaaggcggcg agcgttcctg ccgtcgagct gaactcgggg aagaacaact ccccgaagat
     1261 cagggccgca gccgtgccgt agaggaagaa gtcataccat tctatcgccg tcccgataaa
     1321 gctcgccagc gccacctggc gtatcgaaga cgtccttccc tcttgctctc cacctaatct
     1381 ctccacacga ccctcctttg tgcccggacc ccgatgcgga ctccgaatct ccgatcccca
     1441 ctacccgccg aatactccca ccctcggctc ccagccgcaa cccggaaggt actatggcct
     1501 tggcgctgtg gcctcgatcg agcattggtt gactacgtcc ccggtcgcgg gcttggggac
     1561 gtacccgctc tccggcggtc ggatacctct ctggatgaag ccgaccagcg ccttgaaagc
     1621 gtcccgatgg caggggagta tcgggcgcag cctgtccttg ttgttgtcgt agtacgaatc
     1681 tacgtggttc cccttcccta tcacgtagta cctgtaaatg ttgccgtgcc ccttgtccct
     1741 gacgagctcg gagtatacgt cggagtcggt cctgatgggc agcagcgcgt cgaggtcccc
     1801 gtgcagcgtg agcagcggct tgccgatgtc gccggtcagg gaaaccttcc ttacggcgtc
     1861 cctgacgctc tgcggcctgt cctcgtagac gtagtttgca tcgcaaccag gtatgttggc
     1921 ctcatcctga cactccggga tgccggcctt gagcgccccg tcgtattcgg ggtcgaactc
     1981 ttcgcggtag atgcgctggg tgaggtccca gtaggtcacg tagtgctcgt cccaaaggaa
     2041 ctcggagccc ggctcgaaac cggcgtctat tatgttgtcg tgggcctgct gatcgccggt
     2101 cgcatactca gggtagttgg cgagcgccgt tggcaggtag gtgaagaggt tgggcccttc
     2161 ggcgcgccag agcgttccct cccagtccac gccgccgtcg tagagctcgg gcgtgttctc
     2221 cagggcgtaa cgggtcaggt agcctccgtt tgagatgcca gttatgtagg tccgccgcgg
     2281 cgcctctccg tagtaccgtt tcgcggcgtc cttagtggcc cgcgtcagtt gctcgaccct
     2341 gcgatgccac tcgaggacgg agccgcccgg agcggagccg tcgtcgtaga agctaactcc
     2401 tgtatttccc ttgtcggtcg aggcgaaagc gtatcccttc gccagcaccc agtcgcttat
     2461 gatgaagtcg ttggcgtact gctccctgac accaggggcg ccggttatga cgagcttccc
     2521 gttccagtgg ttggggaacc ggatcacgaa ctgcgagtcg tggagccatc cgttgtttgt
     2581 gttcggcgtc ggcgtcgtcc gcccgtcgtg gaagtaaccg tcaacctgga atcccccaat
     2641 gccactcggg ttgttggtcc cctccgcgtt gagccccgac cagtcgtttt tgtctgtgtg
     2701 cccggtatcc tcggtgcccg cggtcgtcag gtctttgaga cagtctccat cctggacctc
     2761 agcattccga acgaggtgcg gcacctgtct cggcagtttg tgacaatgtc tggagttgct
     2821 gccactatcc tccaccgccc cggcatcagc cgtcctggag accgttgcgg ccacgacgag
     2881 caacgccagc acagcaccag agagcaacgc gacgcacgca cccatcctcg gattcgtcca
     2941 cgatacgcct gtcatggctg atctcccttc ccctcgtcgc aggccgcggc acaccgccct
     3001 cgcatgacct gtactccccc ttgcagtatg gaagaagtct agcgtccacg gtacggccag
     3061 gcaagcgcag cctcaaaaga cctcaaccta cttcagagcg acttcgacga aatgtagccg
     3121 gtcgttaccc cagaacatct ccccgttgac gaagaaggcc ggggcaccga atatgccccg
     3181 ctccaccgcc tccgaggtcg cagccttgag tgcttgcttc gtctcctggc tcgcggctcc
     3241 ctcaagcact tcttcaggtt cggcccctac tctcctggcg acagatgaga tcgggccgtc
     3301 ctcatcggcc tcttcgcgac cctccggcgc gccaccctcc tcccagtaca gcgcgaacgc
     3361 ttcgcgggtg aacgcctcaa gctttcccct ctctcgcaag aacatcgccg cgcgcatcgt
     3421 cttgagggtg cggaatggaa agggttcagg gaactgcagc ggcaacccat agtactcggc
     3481 ccagcgccgg atatcccgcg cctggtagct ggctttgggc tttatctcta tcggggcctg
     3541 cagtcccacc gccttgtgta cagcgccgag cagcatgggc cgcaatgcca gctcagccct
     3601 gttcacttca cagatacgca ctatccgccc gtaggccaga tatgagtagg ggcttacgag
     3661 gtcgtagtaa aactcaaccc gcttcatgcc ggctcctttc gtgctaccag taaggtacag
     3721 cacgtgcgat cttcacgccg tggaaggaag tcacgcaccc tttcgccgta cgatcagaag
     3781 ggaaaccacg atcccaactg ccgcgatccc ggcagcgccg aagaacgccg acctgaaccc
     3841 ttcgacgagc gcggcttcgg ctggcatcgt cccggcgagc gcttccgcgc gggcggcgga
     3901 gaggggaacc aggaccgcga gcccgagaac cgtcccgatc tgggccgccg agttcaagag
     3961 accggaggcg agcccctgca gcccgccgtc cgccgcagag gtcccggaag ccgtcgatgc
     4021 gaccgaggca catcccagac cggagcccat gaggacgaag cctgggatca gataataaag
     4081 gtacccgcca tgacgcgaga tccccaggaa gcataaggtc ccagcggcaa tggcaagcag
     4141 cccgaaggcc atcacggccc tgggccccaa ccacgccacg agcctgggac cgagcaggga
     4201 cccgccgatg accgccaggt tgaacggcgg aaagacgagt cctgcctcag caggcgaata
     4261 cccgaggacc cgctgcacgt agaggatgca caacagcatc ggcggggtag tagaggccgt
     4321 gagcgtgagc gcaacgaggt tcgcgccgag gaaggcacgc gaacggaaga tccccagcgg
     4381 cacgagcggg tacttcaccc tcccctccac cgccacgaag cccgccaaaa acacgaccga
     4441 aacccccaga gacaccaggg tctccggcgc gcccaggccc acctcctgca ctcgtgtgat
     4501 cccatagacg agcaaagtga ggccaacggt tacgctgacc gcgccagcga gatccaacct
     4561 gggagaggca ctaacgtcac ggctctctga gatgaaaaac ggagccagcg ccacaccgat
     4621 aaccccgaca ggcacgttga cgaagaacac ccactcccat cccaaacccc cactcagaag
     4681 tccccccaac aaccagcccg tcgcgccccc tcccgccgcg gcagcggtcc atacgccgag
     4741 cgcgcgcccg cgtccctcac cctcagggaa ggccgtcgtt agtaaggaga gcgcggaagg
     4801 agccacgatc gccgcgccca acccctgcac gacgcgcgca accacgagcg agagcgcgga
     4861 gtccgcaagc ccacaagcca gcgaagcccc cgaaaacacg agcagcccga tcacgaaaag
     4921 cctccgcctc ccccagaggt ccgcagcccg cccaccgagc aacaggaaac ccccgaagac
     4981 aagcgtgtac gccgatatta cccactgaag cccggcctgc gaaaaaccaa gctcatcctg
     5041 tatctccgga agcgctatgg ccacaatggt cacgtcaagc accacaacga actgcgccac
     5101 acacagcaaa cccagtgccg accagcgccg cccctttccc ttgtttccat caaaagcttt
     5161 catactggta acagcatacc cgtagcccgg ggcagtgcaa accggtaaaa tgtgttttga
     5221 tggaaacacc gaggcacatc gatgagttgc ggatagttgg gggacacctc gccctcgact
     5281 tcgtcaacac ggtagaaggg gagcctgacg gcgagcctgg cttcgagttt ctgcgaggct
     5341 atgtagacgt cgtcgcgtgg agcgagcgag tcggcgtgct atccaaagag gaggctgggc
     5401 gtctggctct tgaagcgaag cgcaggcccg aagaagcgga ggcggtatac cgcgatgcgc
     5461 ttgagttgcg ggacacgctc tacggagtct tccgcgccgt cgcggaagga ggaggaccca
     5521 catccgggag cctggaggtc ctgggcgggt acgagtgtga ggccctttcg cgtgggaagc
     5581 tcgcgccggg cgatcacggc ttccgttggg agtggactga cgggggggat ctcgcgagta
     5641 tattgtggcg tgtggcgcat gcggctacgg agctcctgac ctcagggagc ctggatcggc
     5701 tgaagcgctg tgctcattgc cgctgggtgt tcctggacga gagcaggaac cacagccggc
     5761 gctggtgcac catggaagtc tgcggcacgc acgaaaaggt gcgccgctac gtcgcaaaac
     5821 gcgcggctcg acgtagcact atgtgacttc ggcgcggatc catataagaa caaagcggcg
     5881 gggctcggat cgtaatccga accccgctgc gaggccgcta catggaagcg tctggttcta
     5941 ggcgggctgg ctctccgtaa ctgcgcggtc ccggcggagc gccactatcg cgaaggcgat
     6001 ggcagccaac cacacggtga cgatcaggaa gtagccaacg ttggcggcag ctccctgcag
     6061 cccgatggcc ccgaagaacg tctgcatgtt cacaatcagg atgacgccac cgaccaccgt
     6121 ccccaggatt cgcgcgggga gcttcttgac gatgaatgcc gcgagcggag ccgcgatgac
     6181 accacccacg agcagcgctc cgactacctg ccaggggatc tcggcgaaag tcaggctgaa
     6241 gaggaa
//
LOCUS       DATAIR010000614         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_65219, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000614 DATAIR010000000
VERSION     DATAIR010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65219"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..>3192
                     /locus_tag="VHM69_14575"
     CDS             2..>3192
                     /locus_tag="VHM69_14575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2741666.1"
                     /translation="MVSPVVRRKRHPYARLQSTEVQLPDLVEIQLNSFKKFLDEGIQR
                     TFTEISPIEDYTGSYAVEFGEGRFEDPPVSVEECMSKDKTYAAPLFVKVRFIVKETGE
                     VREQDVFMGDFPLMTDSGTFVINGTERVVVTQLVRSPGAYIMEPKDASKQVLTASLMP
                     GRGSWLEFEVETKGYVSVRIDRKRKLPVTVLLKALGMGGPEEILSRFEDSWLVRNTLE
                     RDDTPSREEALLEVFRRQRPGEPVNIENAQNLVEGLFFDAKRYDLSEVGRYKVNKKLK
                     LDVPLENHTLTIEDIEALLARLIETSEEVAEEEEFGRINYERIRHKLDEYEHFGNRRL
                     RTVGELVQEAFRIGMYRMERVVRERMTTQDEESITPQTVVNTKPVASAIKEFFGSSQL
                     SQFMDQTNTLSGLSHRRRLSAMGAGGLSRERAPIEVRDVHPTHYGRMCPIETPEGPNI
                     GLIGQLSTFATVDEYGFVQTPYRKVVNGKVTDEIEYLSADEEEEFTIAQANTPVDPAS
                     GEIAQDAQILARRKGGDVVTVAAEEVDYVDVAPLQTVSVGTALIPFLEHDDANRALMG
                     ANMPKQGVPLLRSEAPYVGTGMEFRAATDTGDVLLARHAGTVERVVSDKITVRREGGE
                     LDEYFLRKFARSNQGTCVNQRPLVKEGEEVEAGTVMADGSSTDQGELALGKNLLVAFM
                     PWEGFNFEDAIIISERLVKDDTLSSIHIEEYEVQARDTKLGPEEITRDIPNASDDVLM
                     NLDVDGIIRIGAEVGSGDVLVGKVTPKGESEPTPEEKLLRAIFGEKAREVKDSSLKVP
                     HGEGGVVIDVKRFSRDGGDELQPGVNEMVRVFVAKKRKIAEGDKLAGRHGNKGVISRI
                     VPEEDMPFMEDGRPVDVILSPLGVPSRMNIGQILETHLGWAASQGLGENDGEPTFVST
                     PVFSGAEVSDIDEAIEKVRNNGGSKTGMGRGGKVTLYDGRTGEPFDGRITVGYMYILK
                     LLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQELLT
                     IKSDDRVGRVKSYESIVKGENIPEPGVPASFKVL"
BASE COUNT          660 a          959 c         1056 g          517 t
ORIGIN      
        1 cttggtgagc cctgtcgtgc gccggaaacg gcacccgtat gctcggttac agtccacaga
       61 agttcagttg ccggatctcg tcgagatcca gctgaattca tttaagaagt ttctagacga
      121 gggtatccag aggactttca cagagatctc ccctattgag gactacacgg gctcttacgc
      181 cgtggagttc ggggaggggc ggttcgagga tccgccggtc tccgtggaag agtgcatgtc
      241 caaggacaag acgtacgcgg cgccgctctt cgtgaaggtc cgctttatcg tcaaggagac
      301 aggagaggtc agggagcagg acgtctttat gggcgacttc ccgctcatga ccgactcggg
      361 gaccttcgtt atcaacggta cggagcgcgt cgtcgtgacg cagctcgtgc gttcgcccgg
      421 cgcctacatc atggagccga aggacgcgag caagcaggta ttgacggcga gcctgatgcc
      481 tggacgcggg tcctggctcg agttcgaggt cgagaccaaa ggttacgtct ccgtgcgcat
      541 cgaccgcaag cggaagctac ccgtcacggt gctcttgaag gcccttggca tgggcgggcc
      601 cgaggagata ctttcgcgct tcgaggattc gtggctcgtg cgcaacaccc ttgagcggga
      661 cgacacccct tccagggaag aggcccttct cgaggtcttc cgcaggcagc ggccaggcga
      721 gcccgtcaac atcgagaacg cgcagaacct ggtggaaggg ctcttcttcg acgccaagcg
      781 ctacgacctc tccgaggtcg gacggtacaa ggtcaacaag aagctcaagc tcgacgtgcc
      841 gctcgaaaac cacacgctca ccatcgagga catcgaggcc ctcctcgcac gcctcatcga
      901 gacctcggag gaggtcgccg aagaagagga gttcggacgg atcaactacg agcgcatccg
      961 ccacaagctc gacgagtacg agcacttcgg caacaggcgc ctcaggaccg tcggggaact
     1021 cgtgcaggaa gcgttccgga tcgggatgta ccgcatggag cgcgtggtac gcgagcggat
     1081 gacgacacag gacgaggagt ccataactcc ccagaccgtc gtaaacacca agcccgtggc
     1141 gagcgcgatc aaggagttct tcggttcctc gcagctctcg cagttcatgg accagacgaa
     1201 tacgctctca ggtctctcgc acaggcggcg tctctcagcg atgggagctg gtgggctcag
     1261 cagggagcgg gcgcccatag aggtgcgcga cgtgcatccg acccactacg gcaggatgtg
     1321 ccccatagag acgccagaag gcccgaacat tggcctcata gggcagctca gtactttcgc
     1381 gaccgtggat gagtacggct tcgtgcagac accgtacagg aaggtcgtaa acggcaaggt
     1441 tacggacgag atcgagtacc tctccgccga cgaggaggag gagttcacga tcgcgcaggc
     1501 gaacacgccc gtcgatcccg caagcggcga gatcgcccag gacgcccaga tcctggctcg
     1561 caggaagggc ggcgacgtcg tgactgtcgc ggcagaagag gtcgactacg tagacgtggc
     1621 gccgcttcag acggtctcag tcggcacggc tctgatcccg ttcctcgagc acgacgacgc
     1681 caacagggcc ctcatgggcg ccaacatgcc gaagcagggc gtaccgctcc tcaggagcga
     1741 ggccccttac gtcgggacgg gcatggagtt cagggcggcg accgacaccg gcgacgtgct
     1801 gctcgcgcgc cacgctggca ccgtcgagcg ggtcgtctcc gacaagataa cggtgcgtcg
     1861 cgagggcggc gagctcgacg agtacttcct gaggaagttc gcccgctcca accagggaac
     1921 ctgcgtcaac cagcgtccgc tcgtcaagga gggcgaggag gtcgaggcgg ggaccgtcat
     1981 ggccgacggg tcatcgaccg accagggcga gctggccctc ggcaagaacc tgctcgtggc
     2041 tttcatgccg tgggagggtt tcaacttcga ggatgcgatc atcatctccg agcgcctggt
     2101 caaggacgat actctctcct ccatacacat cgaggagtac gaggtgcagg cccgcgacac
     2161 caagctcggg ccggaggaga tcacccgcga catccccaac gcttccgacg acgtcctgat
     2221 gaacctggac gtggacggca tcatccgtat cggcgccgaa gtaggttccg gggacgtttt
     2281 ggtcggcaag gtaacgccca agggcgagag cgagccgacg cctgaggaga agctgctgcg
     2341 cgcgatcttc ggcgagaagg cgcgtgaggt caaagactct tccctcaagg tcccccacgg
     2401 cgagggcggc gtcgtcatcg acgtcaagcg ctttagtcga gacggtggcg acgaactcca
     2461 gcccggcgtc aacgagatgg tcagggtctt cgtggccaag aagcgcaaga tcgccgaggg
     2521 cgacaagctc gccgggcgcc acggcaacaa aggcgtcatc tcgaggatcg tgccggagga
     2581 ggacatgccc ttcatggagg acgggcgtcc tgtggacgtg atcctctcac ctctcggcgt
     2641 gcctagccgg atgaatatag gccagatcct ggagacccac ctcgggtggg ccgcgagcca
     2701 gggcctcggg gagaacgacg gcgagcctac cttcgtctcc acgcctgtct tctcaggcgc
     2761 cgaggtttcg gatatcgacg aggccatcga gaaggtgcgt aacaacgggg gctcgaagac
     2821 cggcatgggg cgtggcggca aggtcacgct gtacgacggc aggaccggag agcctttcga
     2881 cggcaggatc accgtcggct acatgtacat actgaagctt ttgcacctgg ttgacgacaa
     2941 gattcatgcc aggagcacag gcccttactc gctggtcacg cagcagccgc ttggcggcaa
     3001 ggcccagttc ggcggccagc gcttcggcga gatggaggtg tgggcgctcg aagcctacgg
     3061 cgcggcgcac accctgcagg agctgctcac cataaagagc gacgaccggg ttggacgggt
     3121 caagagctac gagtcgatag tcaagggcga gaacatcccg gagcccggtg taccggcgag
     3181 cttcaaggtg ct
//
LOCUS       DATAIR010000615         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_411916, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000615 DATAIR010000000
VERSION     DATAIR010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411916"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..442
                     /locus_tag="VHM69_14580"
     CDS             125..442
                     /locus_tag="VHM69_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX2741667.1"
                     /translation="MERNDEWVRVERTPAFKELVRAKKAFIIPATIFFMVFYFGLPFL
                     AAFTTVLNVKVFGPITLAYVYAFAQFAMTWILMHLYVSRANKWDDLVDRARREAAGEG
                     RSA"
     gene            439..2061
                     /locus_tag="VHM69_14585"
     CDS             439..2061
                     /locus_tag="VHM69_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX2741668.1"
                     /translation="MSDQVISIIFFVAIIALTLGITAWASRQNTDTSHHYVAGGDIKG
                     WQNGLAISGDYLSAASFLGIAGAIALTGFSGFYLSIGFLVAYLVVLLLVAEPLRNMGK
                     YTLADMLASRFNTSSVRSVAALSTITISMFYMIAQLNGSGALIGLLLGLPYWLSVIVI
                     GILMTVYVVAGGMVATTWIQIIKAVLLIVGTLTLSILVLARYGFNPIELFSAVENEIG
                     SEAMVPPPPSGLTAGLDVISLNIALVLGTAGLPHILIRFLTVPDAKTARSSIVTATWI
                     IGLFYLLTPVLGYGAALIVGQDEIAKANAAGNLAAPQLAEVLGGPIFFAFISAVAFST
                     IVAVVAGLVVAASSAFAHDFYTNVIRGGQATEQEQFRAARYAAIGISVAAILLALPAE
                     SFNVAFLVALAFAVAASANVPVILLTIFWKRFNTTGAVTGMLVGLISTVVLVLLSPNV
                     LTGPNPEPPATPIIPIDAIFPLLSPAIVSVPLGFLGCYLGTIFGGRGAEREREQGLQV
                     SYDEILVRANTGITNIESEIKEAAPAEEPRTT"
     gene            2150..>2588
                     /locus_tag="VHM69_14590"
     CDS             2150..>2588
                     /locus_tag="VHM69_14590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741669.1"
                     /translation="MDITVRRAEPGDYEALHRIISDPKAIAGTMQLPLPSAEKWRKRL
                     SEPSEDQHSLVACVDGEVVGNLGLETYSTRWRMRHTGRIGMAVRDDWQGQGVGTALME
                     AALDLADNWLNLTRIELHVYVDNAAGIALYEKFGFEVEGTHRRF"
BASE COUNT          466 a          828 c          786 g          508 t
ORIGIN      
        1 tgtgccggag ggccttcgtc cgcccgtgta cccaagcaca tacgaccaat cgtatacgct
       61 tgactgtata caaagcatgc catatagtgc ccagtcaatc ggaggagtgt gggaaaggag
      121 ttttgtggag aggaacgacg agtgggtgcg cgtggagcga accccggctt tcaaggagtt
      181 agtacgcgcc aagaaagcat tcatcatacc ggcgaccata ttctttatgg tgttctattt
      241 tggtctgccc tttctcgcgg cgttcacgac cgttttgaac gtaaaggtgt tcggccccat
      301 cacgctagcc tacgtttatg cgttcgcgca gttcgcgatg acctggatcc tgatgcacct
      361 ctacgtcagc agggccaaca agtgggacga cctggtggac cgggcgcggc gtgaggccgc
      421 tggtgagggg cggtcggcgt gagcgatcag gtcatatcca tcatcttctt cgtcgcgata
      481 atcgcgctga cgctcggcat aacggcctgg gcttctcgcc agaacacgga cacgagccac
      541 cactacgtcg cgggcgggga tatcaagggc tggcagaacg gcctcgccat ctcaggcgac
      601 tacctctcgg cggcctcctt cctcggcata gccggggcca tagcccttac cggtttctcc
      661 ggtttctacc tctccatagg cttcctcgta gcgtacctgg tggtgctttt gcttgtggca
      721 gagccgttgc ggaacatggg caagtacacc ctggcggaca tgctcgccag ccggttcaac
      781 acaagcagcg tccggtcggt ggcggcgttg agcacgataa cgataagcat gttctacatg
      841 atcgcgcagc tcaacggctc gggggcgctc atcgggctcc tgctggggct gccttactgg
      901 ctctcggtca tcgtcatagg aattttgatg acggtgtacg tcgtggctgg cggcatggtg
      961 gcgacgacct ggatccagat catcaaggcg gtgctcctga tcgtcggcac gcttacactt
     1021 tccatcctcg tgctagcccg ctacgggttc aaccctatcg agctattctc cgcggtagag
     1081 aacgagatcg ggagcgaggc catggtaccg ccgccgccca gcgggctcac cgccgggctg
     1141 gacgtgatct cgctcaacat cgccctcgtg ctgggtacgg cggggctacc gcacatcctg
     1201 atccggttcc tcacagtccc tgacgccaag acggcccgct cctcgatcgt tacggcgacc
     1261 tggatcatcg gcctcttcta cctgcttact ccggtgctcg gatacggcgc agcgctgatc
     1321 gtcggccagg acgagatcgc caaggcgaac gccgcaggca acctcgcggc cccgcagttg
     1381 gccgaggtcc tggggggtcc gatcttcttc gcctttatct cggcggtcgc cttctccacc
     1441 atcgtggccg tggtcgctgg cctcgtcgtc gcggcctcca gcgccttcgc ccacgatttc
     1501 tacaccaacg tgatccgtgg cgggcaggcc accgagcagg agcagttcag agcggcgcgc
     1561 tacgcggcca tcggtatctc cgtcgcggcg atactccttg cgctacccgc ggagagcttc
     1621 aacgtagcgt tcctggtcgc cctggccttc gcagtcgccg cgagcgcgaa cgtgcctgtc
     1681 atcctcctga cgatcttctg gaagcggttc aacaccacgg gggcagtcac aggcatgctc
     1741 gttggcctga tctccaccgt cgtgctcgtc ttgctcagcc cgaacgtgct taccggcccc
     1801 aaccccgagc cgccggccac cccgatcatc cccatagacg ccatcttccc gctcctgagc
     1861 ccggctatcg tctcggtacc acttggcttc ctgggatgct acctcggcac gatctttggc
     1921 ggtcgaggcg cggaacgcga gcgagagcag ggactccagg tttcctacga cgagatcctg
     1981 gtccgcgcca acacaggcat caccaatata gagagcgaga tcaaggaagc agccccggca
     2041 gaagaacccc gcacgactta gtgaaataaa gcgctcgact ggagccctcg ccactaaccg
     2101 gcgggggctc ttcttgtatc cttagcgggc acgcacaggg aggacgaata tggacatcac
     2161 cgtccgacgc gcggagcccg gagactacga ggcgctgcac cgcatcataa gcgacccgaa
     2221 ggcgatcgcc ggcaccatgc aactcccgct cccttcggct gagaagtggc gcaaacgtct
     2281 ctccgagccg tcagaggacc agcattctct ggttgcctgt gttgacggcg aggtggtcgg
     2341 aaacctcggc ctggagacct attcgacccg ctggcgtatg cggcacacgg gtcgcatcgg
     2401 gatggccgtg cgcgacgatt ggcagggcca gggcgtcggg acggcgctca tggaggcagc
     2461 gctggacctc gcggacaact ggctgaacct gacgcgcatc gagctgcacg tgtacgtgga
     2521 caacgccgca ggtatcgcac tgtacgagaa gttcgggttc gaagttgagg gaacccaccg
     2581 ccgcttcg
//
LOCUS       DATAIR010000616         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_411974, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000616 DATAIR010000000
VERSION     DATAIR010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411974"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VHM69_14595"
     CDS             <1..762
                     /locus_tag="VHM69_14595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2741670.1"
                     /translation="KDTTPEDLCDWDKMCDAIAEAEPWWEPGTKVAYHAYPFGYIVGE
                     IVRRTTGKPISQVLREEVAVPLGIADEIYFGVPESELGRLARLEDVEGSAEFLASMPD
                     DAPFFKLGPRTVTPTAEFGNRADILMADIPAGGKMSARAVARMYAALMDEVDGVRLVS
                     PERLREISAGAFTGIDQIMGFPSTWALGYSVGRIGADPQETSSVLGVGGVGGSFACAD
                     TATGVAWAVTKNRLTADFSAAEQISGIVANATAES"
     gene            797..1366
                     /locus_tag="VHM69_14600"
     CDS             797..1366
                     /locus_tag="VHM69_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741671.1"
                     /translation="MRKLVAVELVSLDGVMESPEEWAFPYTDDEMNEANATGMAASDA
                     LLLGRKTYEALAAFWPDQPGGTPMVDYINSVRKFVVSTTLEDDLEWNNSSLIGGGEGN
                     VAEEIADLKRQPGKAITVLGSGVLVRSLLREGLLDELTLMVHPIVLGSGKRLFDGDGE
                     RKVLELVDATAFGSGVVSLVYRPSAGQDS"
     gene            1390..1803
                     /locus_tag="VHM69_14605"
     CDS             1390..1803
                     /locus_tag="VHM69_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX2741672.1"
                     /translation="MNWKIEVVTIPVSDLDRSIEFYAEKVGFNVDIDFKVSDEVRLVQ
                     LTPPGSACSIHLGKNTVDMAPGSIDGVFLVVPDVRAAHAELAGRGVEVGEIQIFEGGR
                     YRPAREGEDLNNVGCIFFNDPDGNRWCVQQIPPRG"
     gene            1824..2435
                     /locus_tag="VHM69_14610"
     CDS             1824..2435
                     /locus_tag="VHM69_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741673.1"
                     /translation="MGKVAMGLTMSLDGFIAGPNDGPEHPLGEGGMRLFDWYSSGDIE
                     YHMAGTEMVVRVSRQSADMLREAHSKMGAFVTGRRTFDITNGWGGNPPLGVPAFVLTH
                     SVPEEWVYEGSPFTFVTEGVESAVEQAKKAAGEKDVAVGAASIVQQCIRAGLLDEIHI
                     DLVHVLLGDGVRLFDNLGGQVELERTEIIEAPDVTHMTFRVVR"
     gene            2449..3000
                     /locus_tag="VHM69_14615"
     CDS             2449..3000
                     /locus_tag="VHM69_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2741674.1"
                     /translation="MRKLVVSEFLSLDGVVEDPSWSFPFESAEKDRFKFDELAAAGGL
                     LLGRVTYEGFAAAWPQLEEETGEYGAWMNGYPKHVASTTLQEPLEWNNSSLIQGDVAE
                     KVTELKQGSGGDILVFGSGDLVNTMMQNDLVDEYRLMIFPVVVGKGKRLFGEGSGTKT
                     MRLLDTKAFSSGVVVLTYGPAGE"
     gene            3074..3214
                     /locus_tag="VHM69_14620"
     CDS             3074..3214
                     /locus_tag="VHM69_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741675.1"
                     /translation="MTRRLLACGVIAGPLYVIGAGIFIADPMGGFPPGTPQNAGTVSC
                     TS"
     gene            3758..5701
                     /locus_tag="VHM69_14625"
     CDS             3758..5701
                     /locus_tag="VHM69_14625"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX2741676.1"
                     /translation="MTEMVVPGTYITVRAEGLISAGRVATGIVGVVGTAANGPIGVPV
                     TLSGLTDARARFGLPDDFDRPEDGANPLTLFRALEHIYSNGAASVVAVRVAGSSQSSA
                     TYTVQDADGLNVAVLSASTPGTWANNIRVQIDPAEDPCRVDDETHTEDFDQLEYGSIR
                     PSPENRLRITRGATRRTETPDIVYRRVVRDEEVVRRNNRFFLAGVDADTPVFEVPDIN
                     LIRVLEDDEPVREYGDGDILYGAGGAPGAGEVRLDNATGELTFEATEVPTGTQTVVAT
                     YALDHPEPQSGEVRVTTWDGSLDFAEDEAPDEVEGDTLVASYLIEADACVQVTLTYGA
                     VIERYTVPDGNLLASQVNSSSALVSAEADDDDGGNLPRTGVDAYFGTGVNVPGSNGAE
                     AGRDEYTLGLEALSDRLVNIVVLAGQDAGTMGSALLGHLNSTEQTDFERIGVIGAAGE
                     STADFLGHTMADDRVILVAPGIREPGGTVLPPAYTAAAVAGLISSFPVQTSLTNKPLN
                     VPGLANRFNRGEQGQLIRRNVLAVVERDGFRVLKGVTTEGEGAPFSAVPTRRIVDYAK
                     YGVRSAANPYLGRLNNVRVRAALQSTLDAFLTRMVEDEALTGYELQVSATRAQEIAGE
                     VSVVMTLQPTFSIEFIRVIMNLS"
     gene            5738..6241
                     /locus_tag="VHM69_14630"
     CDS             5738..6241
                     /locus_tag="VHM69_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741677.1"
                     /translation="MPNDVFRAIDATLVLGVEDASLPEGTVADALVSEYDLSNTVGRL
                     HQVTVSVLSDVQPFYEIGRRYPTHLRPGVVRVSGSAERAHVNGALLRLLLGEGAVSPP
                     GASNFVQPAFNIVASLQDPARPDQTTRITVFGTKFDAWDYHVATEDSFVMEQVTFQAL
                     RIAFEES"
     gene            6275..>6550
                     /locus_tag="VHM69_14635"
     CDS             6275..>6550
                     /locus_tag="VHM69_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741678.1"
                     /translation="MVHAETQAILDAQDLLAGSAIIHDVRVPGEILRPGAEALDGDGV
                     VRMKPLSVAVLTLVSRAAREDPGLIPLLMIKESLVEPVLALDQIRSMH"
BASE COUNT         1303 a         1978 c         2166 g         1103 t
ORIGIN      
        1 aaagacacga cgcctgaaga cctgtgtgac tgggacaaga tgtgcgatgc gatcgctgag
       61 gccgagccgt ggtgggagcc tggcacgaag gtcgcctatc acgcgtatcc gttcggctac
      121 atcgtagggg agatcgtacg ccgtactacc ggcaagccca tctcgcaggt gctccgcgag
      181 gaggtagccg ttccgctcgg catcgccgat gagatctact tcggagtccc cgagtccgaa
      241 ctgggcaggt tggcgaggct ggaggacgtc gaaggcagtg cggaatttct cgcctcgatg
      301 cccgatgacg cgccgttctt caagctgggg ccgcggaccg tgaccccgac cgccgagttc
      361 ggcaaccgcg ccgacatact gatggcggac atccccgccg gtggcaagat gtcggcgcgt
      421 gccgtcgcgc gaatgtacgc cgccctgatg gacgaggttg acggcgtcag gctcgtctcg
      481 cccgaacggc tgcgcgagat atctgccggg gcgttcaccg ggatcgacca gatcatgggc
      541 ttcccatcca cctgggcgct cggttactcg gtcggtcgta tcggggcaga cccgcaagag
      601 acgagtagcg tactcggcgt cgggggcgtc ggcgggagct tcgcgtgcgc cgacaccgca
      661 acgggcgtcg cgtgggccgt gacgaagaac aggctcaccg ccgatttctc agccgcagaa
      721 cagatctccg gcatcgtcgc gaatgccaca gccgaaagct aatccgcgtt ataaagccag
      781 ggaaaggagg gccagagtga ggaaactcgt cgcggtggag ctggtgtcgt tggacggcgt
      841 catggaatcg cctgaagagt gggctttccc ctacactgat gatgagatga acgaggcgaa
      901 cgcgacggga atggccgcgt cggacgcctt gctgctggga cgaaagacgt acgaggctct
      961 ggccgccttc tggcccgacc aacccggtgg cacgccgatg gtggactata taaacagcgt
     1021 gcgcaagttc gtcgtctcga cgacgcttga ggatgacctg gagtggaaca actctagttt
     1081 gatcgggggt ggcgaaggaa acgtcgctga ggagatcgcc gacctgaagc ggcaacctgg
     1141 caaggcgatc accgtcctcg gaagtggcgt cctcgtgcgg tcgctgctgc gcgagggatt
     1201 gctcgacgaa ctgacgctca tggtgcatcc catcgttctc gggagcggta agcgtctctt
     1261 cgacggcgac ggagagcgga aggtactaga actggtggac gcgaccgcgt tcggctcggg
     1321 agtcgtatct ctcgtctacc ggccttcggc gggacaggat tcttgaaaga cagtagaaag
     1381 gagagcgaca tgaactggaa gatagaggtc gtgacgatcc cggtatccga cttagaccgc
     1441 tccatcgagt tctacgcgga gaaagtcggc ttcaatgtgg acatcgactt caaggtcagc
     1501 gacgaggtcc gtctcgtcca gttgacccca ccagggtctg cctgttcgat ccacctcgga
     1561 aagaacaccg tggatatggc gcctggttcc atcgatggcg tgttcctggt cgtccccgac
     1621 gtgcgcgccg cccacgccga gctagccggg cgtggcgtcg aggtcggcga gatacagatc
     1681 ttcgaaggag gcaggtaccg cccggccagg gagggagaag atctcaacaa cgtcgggtgc
     1741 atcttcttca acgaccccga cggcaaccgc tggtgcgtgc aacagatacc accacgcggc
     1801 taggtcgcag aaaggagaac gagatgggga aagtagccat ggggctcacg atgtcgcttg
     1861 acggctttat cgcggggccg aacgacggcc ccgagcatcc gctcggcgag ggtgggatgc
     1921 ggctcttcga ctggtattcg agcggcgata tcgaatacca catggcgggt accgagatgg
     1981 tggtcagggt ctcgcggcag agcgccgaca tgcttcggga agcgcacagc aagatggggg
     2041 cgttcgtgac gggacggagg acgttcgaca tcaccaacgg gtggggtgga aatcctccac
     2101 tgggagtacc ggccttcgtc ctgacccatt cagtcccgga agagtgggtc tatgagggat
     2161 ctcctttcac gttcgtcacc gaaggcgtcg agagcgccgt cgagcaagcc aagaaggcgg
     2221 cgggcgagaa ggatgtcgcc gtcggcgccg cgagcattgt gcagcagtgc ataagggccg
     2281 ggctgctcga cgagatacac atagacctcg tgcacgtcct gctcggggac ggtgtccggc
     2341 tcttcgacaa cctcggcggt caggtggaac tcgaacgcac ggagatcatc gaagcccccg
     2401 acgtgaccca catgaccttc cgggtcgtca ggtgaaagga gaaccgacgt gagaaaactg
     2461 gtcgtatcgg agttcctctc gctggacggt gtggtggaag acccgagctg gtccttcccg
     2521 ttcgagagtg cggagaagga caggttcaag tttgatgaac tggccgcggc cggcggcctc
     2581 cttctggggc gcgtgactta cgaaggcttc gccgccgcct ggccgcagtt ggaggaggag
     2641 acgggcgagt acggagcctg gatgaacggc taccccaagc acgtcgcctc gacgaccttg
     2701 caagagcccc tggagtggaa caactccagc ctgatccaag gcgacgtcgc ggaaaaagtg
     2761 acggaactca agcaagggtc cggtggggac atcctggtct tcggtagcgg agacctcgtg
     2821 aacacaatga tgcaaaacga cctcgtcgat gagtacaggc tcatgatctt ccctgtcgtc
     2881 gttgggaagg ggaagcgtct cttcggggag ggcagcggca cgaagacgat gaggctcctg
     2941 gatacgaagg ctttcagttc tggcgtcgtc gtccttacct acgggccggc gggcgagtag
     3001 ggagtcctgc acagagagtt gcaagtgtca ggaggcggcc tgtgagccgg gggcttggag
     3061 cgaggggagc aaagtgacga ggcggctgtt ggcctgcggg gtcatcgcgg gtccactgta
     3121 cgtgatcggc gccgggatct tcatcgccga tccgatgggc ggctttcctc ctggtacccc
     3181 gcagaacgcc ggcacagtct cctgcacttc gtaaccggcg ggataggatt cctcgggctg
     3241 atctcggcgt gtttcgtcta cgcccgccgg ctcgcggcca tcggcatgcc aagcgagggc
     3301 cgtacctggg gctcttcgcc aaaaaagcac cagcccgaga acgtcgttgc gacggcgagc
     3361 gcctgtcatc tcctgaatac gtctgccttg ccggccgtcg gcgaccacct tttgtattgc
     3421 gtcggatctc cagggcaggc tatccgagat aggcggtaaa tccggggaaa tcggaaaaat
     3481 tcatccgccc atcatcataa attcatcgga aaatcatcgc ctgctgctta aattctcacg
     3541 ccaatactgc ggaaaaggct ctcccgggcg gtgagtcagg actgggggag caagcgagcg
     3601 acgaggaggc gacgatcgaa gagcaacccc gagtcggcgg tttcgagtac cgaagggaga
     3661 aagccaccac cacattctct ggcgagagcg ggacggaccc cacggttctc tcgtagacac
     3721 ccaccatcga ttcaagaaat caagggagac caaaaccatg actgagatgg ttgttccggg
     3781 gacctacatt acggttcggg cagagggttt gatctcggcg ggcagggtgg ctacgggcat
     3841 cgtcggggta gtcgggacgg ccgccaacgg tcctatcggg gtaccggtca ccctctccgg
     3901 cctgaccgac gcgcgggcca ggttcgggct ccccgacgat ttcgaccgtc cggaagacgg
     3961 cgccaacccg ctcacgctct tcagggcgct ggagcacatt tacagcaacg gcgcggcttc
     4021 ggtggtcgcg gtcagggtcg cggggtcgag ccaatccagc gcgacgtata ccgtacagga
     4081 cgccgacggg ctaaacgtcg ccgtgctgag cgcaagtacg ccaggcacgt gggcgaacaa
     4141 catcagggta cagatagatc ccgcggagga cccttgccgg gtcgatgacg aaacccatac
     4201 cgaggacttc gaccagctgg agtacggctc gatcaggccc tcgccggaga accggctccg
     4261 tatcaccagg ggcgcgacga ggcgcacgga gacaccggat atcgtctaca ggagggtcgt
     4321 tcgggacgaa gaagtcgtca ggcgaaacaa ccgtttcttc ctcgccggcg ttgacgcgga
     4381 cacacccgtc ttcgaggtcc ctgacatcaa cctgatccgt gttctcgaag acgacgaacc
     4441 cgtcagggaa tacggggacg gcgacatcct ctacggcgcc ggaggagctc ctggcgccgg
     4501 tgaggtcagg ctggataacg ccacgggcga gctaacgttc gaggccaccg aagttcccac
     4561 tggcacccag acggtcgtcg ccacgtacgc cctcgatcat cctgagccgc aatccggaga
     4621 agtgcgcgta accacctggg atgggagcct cgacttcgcc gaggacgaag cgccggatga
     4681 ggtcgagggc gacacgctag tcgcctcgta cctcatcgag gccgacgcgt gcgtacaggt
     4741 gacgttgacg tacggggccg tgatcgaacg gtacaccgta cccgacggca acttgctcgc
     4801 gtcgcaggtc aacagttcgt cggccctcgt ctctgccgag gccgacgatg acgacggggg
     4861 caaccttcct cggacgggcg tcgacgcgta cttcggtacc ggcgtgaacg tgccagggag
     4921 caacggcgcc gaagccggtc gggatgaata cacgctgggc ctggaagcgc tctcggacag
     4981 gctcgtcaac atcgttgtcc tcgccggaca ggatgcgggg accatgggct cggccctcct
     5041 cggccacctg aactccaccg agcagaccga cttcgagcgc atcggagtca tcggcgcggc
     5101 gggggagagc accgcggatt tcctcgggca caccatggcg gacgaccgtg tgatcctcgt
     5161 cgcgccaggc atacgcgaac cgggcgggac cgtactccct cccgcctaca ccgccgcggc
     5221 ggtcgctggt ctgatctcct ccttcccggt gcagaccagc ctgaccaaca agccgctaaa
     5281 cgttcccggg cttgccaaca ggttcaacag gggcgagcag gggcagctga tccggcgcaa
     5341 cgtcctcgcc gtcgtcgagc gcgacggctt cagggtgctc aaaggcgtca ccacggaagg
     5401 cgaaggagcg ccgttctccg cggtgcccac gcgccggatc gtggattacg ccaagtacgg
     5461 cgtccgctcc gcggccaatc cctacctggg ccgtctgaac aatgtgcgcg tccgcgccgc
     5521 cctgcaatcc acactcgacg ctttcctcac ccgcatggtc gaggacgagg ccctgacagg
     5581 gtacgagttg caggtttcgg ccacgcgagc ccaggagatc gcaggagagg tcagcgtcgt
     5641 gatgaccctc cagccgacct tcagcataga gttcatcagg gtgatcatga acctgagcta
     5701 gtgccgcatc caaacctctg gagaggagtg acaactcatg ccgaacgatg tctttcgggc
     5761 aattgacgcc acgctggtgc tcggtgtcga ggacgccagc ctgcctgagg ggaccgtcgc
     5821 cgacgccctg gtgtccgagt acgacctgag caacaccgtg ggccgacttc accaggtgac
     5881 cgtgtcggtc ctcagcgacg ttcaaccgtt ctacgagata ggacgccgtt atcccaccca
     5941 cctgcgcccc ggcgtggtac gcgtgtcggg cagcgcggaa agggcccacg tcaacggcgc
     6001 cttgctacgg ctcctgctcg gagagggtgc tgtcagcccg cctggagcct ccaacttcgt
     6061 ccagccggcc ttcaacatcg tcgcaagcct tcaagacccg gcgcggcccg accagacgac
     6121 caggatcacc gtgttcggta cgaagttcga cgcctgggac taccacgtcg ccacagagga
     6181 cagcttcgtg atggagcagg tgacgttcca ggcgctgcgc atagcgttcg aagagagcta
     6241 agaaacccca acaaccggag ggacttacga gcagatggtc catgccgaaa cgcaagccat
     6301 actagacgcc caggatctgc tggcggggag cgccatcatc cacgacgtca gggtgcctgg
     6361 tgagatcctg aggccaggag cagaggcgct ggacggagac ggtgtcgtca ggatgaagcc
     6421 gctgagcgtc gcggtcctca cgctcgtctc acgcgcggcg cgggaggacc ctggcctgat
     6481 acccctgctc atgatcaagg agtcactcgt cgagcccgtc ctcgcgctgg accagatccg
     6541 cagtatgcac
//
LOCUS       DATAIR010000617         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_457348, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000617 DATAIR010000000
VERSION     DATAIR010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457348"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VHM69_14640"
     CDS             <1..833
                     /locus_tag="VHM69_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2741679.1"
                     /translation="ALGGALAKGSGSMGFSIGEVSQAVGLAPQTLRLWERERLITPRR
                     TDRGYRVYTEPDVARLRQIKQLRKVEGLNFAAIRKHLGSAPEKPGSKDSDTAGPSEAP
                     GERLRRLRVKAHKTLKEVSETTGLSISFISALERGGSGASVASLRLLAGAYGVNMREV
                     FGADLEQTSPLVRSEDRPVMQWDNGVRFEEMASGEKVMDPSYIRVPPGAGSEGFYSHT
                     GEEFVYVVSGSLFVELKDQGTFRVAPGDTLYFPSTTPHRWWAGDEPVEAVYVNTPPTF
                     "
     gene            843..>2321
                     /locus_tag="VHM69_14645"
     CDS             843..>2321
                     /locus_tag="VHM69_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2741680.1"
                     /translation="MPRDLLDALLSIPALSGPVVSPDGVWVAWSWSRLGPAADVFAAP
                     ADGSRPPLRLTETVEGDTIVVSWTPDGEAVLVTQDRDGDERARLFRVRLAEPCAMEPL
                     TEPEPNYYLGGGQLHPDGRWLIYSANLDAQSGAEIETDRLYRHDLLTGERKVLAWPEK
                     GSIFEPELNRPGTHVLYARNDLDPAGQQVWLVDIEGRKDREILNFGPRVKVSACWLPD
                     GRRVLFVAETGSYRRLGLWSMDEEPIRWLVDDPARNIEDAFAPPNGDRVVVVEVEQAG
                     VRASLLDVESGAEIRLEHTTGNLVPLAPADEGHWICTYYDARHPVDLVRYSTEDGGPA
                     SVTGLPELTPVGAERLVAAEDFRWRSVDGLEVQGWLYRTPGESPGTIVLIHGGPTSRA
                     EDRFNAEIQYLLTRGFNVLAPNYRGSTGFGLPFQESIKDDGWGGREQEDIRSGIAALI
                     EAGVAQPGRIGVTGTSYGGYSAWWAITHFEPHVAAAPICGMTD"
BASE COUNT          415 a          719 c          806 g          381 t
ORIGIN      
        1 cggcattggg aggagcgttg gcgaagggaa gcggttcgat ggggttctcc ataggcgagg
       61 tctcgcaggc ggtaggtcta gcgccgcaga ccctgcggtt atgggagagg gagcgcctga
      121 tcacaccccg caggacggac cgtggctaca gggtgtacac ggagcccgac gtggcgcggt
      181 tgcgccagat taaacagcta aggaaggtcg agggccttaa cttcgcggcg atacgcaagc
      241 acctcggcag cgcgcctgag aagcccggct ccaaagactc ggatactgcc ggaccgtccg
      301 aagctccggg ggagcggttg cggaggctca gggtcaaagc ccacaagacg ctgaaggagg
      361 tttcggagac cacggggctc tcgatctcct ttatctcggc gctggagcgc ggaggctcgg
      421 gggcttcggt cgcttctttg cggctgctgg caggggccta cggggtgaac atgcgcgagg
      481 tgttcggcgc agacctggag cagacctcgc cgctggtgcg gagcgaggat cggcctgtga
      541 tgcagtggga caacggcgtg cgcttcgagg agatggcctc gggggagaag gtcatggacc
      601 cttcctacat cagggtgccg cccggtgcag gcagcgaggg tttctactcc cacaccgggg
      661 aggagttcgt atacgttgtc tctggttccc tgttcgtaga gctcaaggac caggggacgt
      721 tccgggtagc gcccggcgac acgctgtatt tcccttccac cacaccgcac cggtggtggg
      781 caggcgatga gcccgtcgaa gccgtgtacg tcaacacccc gccaacgttc tagccggtcc
      841 tcgtgccccg agacctcctc gacgcgctgc tctccatccc ggctctctcc gggccggtgg
      901 tctcgcccga cggagtgtgg gtggcgtgga gctggtcgcg gctcggaccc gccgccgacg
      961 tgttcgcagc gcctgccgat ggctcgcggc ctccactccg tctgacggag actgtagagg
     1021 gagacacgat tgtggtctca tggactcccg acggcgaagc agtcctggta acgcaggaca
     1081 gagacgggga cgagcgcgcc agactcttca gggtgaggct tgcggaaccc tgtgcgatgg
     1141 agccgcttac agagccggag ccgaactatt atctgggagg cggccagttg caccccgacg
     1201 gacgctggct aatctactcc gcgaacctcg acgcccagag tggagcggag atcgagaccg
     1261 accggctcta tcgccacgat ctgctgacgg gcgagagaaa ggtgcttgcc tggcctgaga
     1321 agggcagcat cttcgagccc gagctgaacc gtcccggcac ccacgtactc tacgcgcgca
     1381 acgacctgga ccccgcaggg cagcaggtat ggctggtgga catagaaggc cggaaagacc
     1441 gtgagatcct gaacttcggc cctcgggtca aggtctcggc ttgctggctc cccgacggac
     1501 gcagggtcct cttcgtcgcc gaaactggct cctaccgcag gttgggcttg tggagcatgg
     1561 acgaagaacc catccgctgg ctcgtagacg accccgcccg caacatcgag gacgccttcg
     1621 cgccaccgaa cggagaccgg gttgtcgtag ttgaggtcga gcaggccgga gtgagggcct
     1681 cactgctcga cgtggagagc ggtgccgaga ttcgtctaga gcacaccacg ggcaacctgg
     1741 tgcctctggc cccggccgat gaagggcact ggatctgcac ttactacgac gcccgccacc
     1801 ctgtggacct ggtccggtac agcacggaag acggcgggcc agccagcgtc accggcctgc
     1861 cagagctgac gcccgtaggt gcggagcgac tcgtcgcggc tgaggacttc cgttggcggt
     1921 ccgtggacgg tctcgaggta cagggatggc tctacaggac ccccggcgag agccctggaa
     1981 ccatagtctt gatccacggc ggcccgacca gtcgcgccga ggaccggttc aacgccgaga
     2041 tccagtacct cctcacgcgc ggcttcaacg tgctcgcgcc taactacagg ggtagcaccg
     2101 ggttcggcct ccctttccag gagtcaataa aggatgacgg ctggggcggt cgggagcagg
     2161 aagacatcag gagtgggatc gcagcgctca tcgaggctgg cgtggcgcag ccggggcgca
     2221 tcggtgtcac gggcacctcc tacggagggt actcggcgtg gtgggcgatc acacacttcg
     2281 agccgcacgt cgccgccgcc ccgatctgcg gtatgaccga c
//
LOCUS       DATAIR010000618         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_250093, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000618 DATAIR010000000
VERSION     DATAIR010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250093"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..327
                     /locus_tag="VHM69_14650"
     CDS             106..327
                     /locus_tag="VHM69_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741681.1"
                     /translation="MVSVCIEVGSETGSHKVAVRARSIERAVRIVAQRYPGSRVEVEF
                     PIDPETFFAEGPFPEGVISADKSAQRRAA"
     gene            324..809
                     /locus_tag="VHM69_14655"
     CDS             324..809
                     /locus_tag="VHM69_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741682.1"
                     /translation="MRQSRVAVREAGWTAHVPEEYGTEAEGRNASFWDRILGLLEEDL
                     ARRRERKIFVKMADGTGSYEEIGDLSTEQLRSLDRGLHGWVAEQLLDRYLAEGGYVDL
                     LGRRRAYTKRTKDLVPEDLPALVAADRAKAEEHSEGRDRARFAADVHRDLERLGTRLP
                     G"
     gene            complement(835..1506)
                     /locus_tag="VHM69_14660"
     CDS             complement(835..1506)
                     /locus_tag="VHM69_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741683.1"
                     /translation="MEAEGTHKNPTRVLIADDHALFRFGLKAALRAEEDFEVVGEADS
                     GESVVSLAAQLKPQIILMDIQMPGFNGIEATRRILEADPDVVVVVVTMFEEDDSVFAA
                     MRAGARGYVLKEDDADEMIRVIRAVAEGEAHFGQEIARKLRGFFSRPTAPKEVFPELT
                     PREGEVLDMIARGLNNAEISRRLYVSPKTVRNHVSNIFLKLQVADRSQAIVRAREAGL
                     GRDGP"
     gene            complement(1511..3682)
                     /locus_tag="VHM69_14665"
     CDS             complement(1511..3682)
                     /locus_tag="VHM69_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2741684.1"
                     /translation="MNRWSTLPGTSVHPAPPRTELHGRPLLAARSAWIVLTVLIVGLS
                     AASVPGAYSEYSSVCAAGAECRPYWRIAPEDLRALHDLGISVSSYAVYRLTADILYGL
                     GFWAIGAVIFWRKSDNWVALFVSVMLVTFGALNMIELASGTVPLLARVDAVLSFLGYV
                     AFVVAFYLFPDGRFIPGWTRWLVIVWTLYLASLYFLPDRSPYPGAWSSLLSAPIVISL
                     LGALVFAQAYRYRRVSGPVERQQTKWVVLGLTSAIILSVLVILPAVFYPELLQPGAPK
                     IMYALFELTATNLVLLLIPLSIGVAILRYRLWDVDVVINRTLVYGALTASVAGIYVLI
                     VGSLGAILQARGSLAISLPAAGVVAILFAPLRQRLQSGVNRLMYGERDEPYRVLSRLG
                     ERLEATLEPASVLPTIVETVAAALKLPYAAISMARESGYEVAAAKGTPTGEETALPLT
                     YGGRPIGELTLAPREPGDAFTPADMRLLRDLARQAEVAVHAVRLTADLQRSRERLVTA
                     REEERRRLRRDLHDGLGPTLAGLSLGLDISLKTLRSEPGEAEHLLAELKSRTQEAVAD
                     VRRLVYGLRPPALDELGLVLAIREQAAGYGYLADDSEKLREDDTAFYVEAPEELPPLP
                     AAVEVATYRIVQEAITNVARHARARSCLVRLAIHDARDELVLEVSDDGVGLPDGRRAG
                     VGSSSMSERSEELGGTCTVSSNPAGGTRVIARLPLFAEDRG"
     gene            complement(3780..4346)
                     /locus_tag="VHM69_14670"
     CDS             complement(3780..4346)
                     /locus_tag="VHM69_14670"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2741685.1"
                     /translation="MTIEENKAVVRRFWEQAFDSGDLELVDKLFAQDHALRYPGLGTE
                     GEGRDAMKALVSLFRSVSPDFRINVQDEIAEEDRVVVRWAGSGSVREEIAAEAGDMVR
                     VSGIAISRVGRGRIEETWLRFTAHPDGIQRQVPREGILERVGLDISDPAVLAGRLICL
                     IIPRACVPDGERFPEDPTGPHGLTRPDG"
BASE COUNT          931 a         1494 c         1491 g          842 t
ORIGIN      
        1 cgtggttcgc gtctgggcgc cctcgcccga acggcgacgc tgccgctgct tcgtagagcg
       61 agaccctcgg gatagcggtt cggagtgtaa aggaggtaat cgctcatggt gagcgtctgt
      121 atagaagtcg gcagcgagac aggtagccac aaggtggccg ttcgggcccg aagcatcgag
      181 cgggccgtac gcatcgtggc ccagcggtat cctggcagcc gcgtcgaggt cgagttcccg
      241 atagatcccg aaactttctt cgccgaaggt ccctttccgg agggtgtgat ctcggcggat
      301 aagagcgcac aaaggagagc ggcatgagac agagcagggt tgctgttcgc gaggctggct
      361 ggacagctca cgtacctgag gagtacggga ccgaagcaga gggacgaaac gcatcctttt
      421 gggaccggat actagggttg cttgaggagg atctggcaag gcggcgcgaa cggaagattt
      481 tcgtgaagat ggcggacgga accggatcgt acgaggagat cggcgatctg tccacggagc
      541 aactccgcag cctggaccgc ggactgcacg ggtgggtcgc agagcaacta ctcgacaggt
      601 atctggcgga aggcgggtat gtggatctcc tcgggagaag acgcgcctac accaagagga
      661 ccaaggatct cgtaccagag gacctgccgg ctctcgtggc agccgacagg gcgaaggccg
      721 aagagcattc cgaagggcga gaccgggcga ggttcgccgc cgacgtgcat cgggacctgg
      781 agcgcctggg tacacggctt ccgggctaga actaagggga tagaggcagc actatcaggg
      841 tccatccctc cccagtccgg cctctcgggc acggacgatg gcctgggagc ggtcggcgac
      901 ctggagtttc agaaagatgt tcgagacgtg gttgcgcact gtcttggggc tgacgtagag
      961 gcggcgggag atctcggcgt tgttcaggcc tcgcgctatc atgtcgagga cctcgccctc
     1021 cctgggggtg agctcgggaa agacttcttt gggggcggtg ggccttgaga agaaacccct
     1081 cagcttcctc gcgatctcct ggccaaagtg ggcctcccct tctgcgacgg cgcgtatcac
     1141 cctgatcatc tcgtcggcgt cgtcctcctt gagcacgtag ccgcgcgcgc ctgcgcgcat
     1201 ggcggcgaac accgaatcgt cttcttcgaa catggtcacg accacgacca cgacgtccgg
     1261 gtctgcctcg agtatgcggc gcgtagcttc tatgccgttg aaccccggca tctgtatgtc
     1321 catgaggatt atctggggtt tgagttgggc ggccagcgag acgacgctct cacccgagtc
     1381 cgcctcgccg acgacctcga agtcttcttc ggcgcgcagc gcagccttga gcccgaagcg
     1441 gaatagagcg tggtcgtcgg ctatcaggac gcgcgtcggg ttcttgtgtg ttccttctgc
     1501 ttccatctcg ttaccccctg tcttcggcga acagtggaag gcgggcaatg acgcgggtcc
     1561 cgcccgccgg gttcgatgac accgtgcacg tgccgcccag ttcttcggac ctctcggaca
     1621 tggaagagga gcccacgcca gcccggcggc cgtcgggtag gccgactccg tcgtcggaga
     1681 cctccaacac gagttcgtcc ctggcgtcgt ggatggcgag acgtaccagg cagctcctgg
     1741 ccctggcgtg acgggcgacg ttggtgatcg cctcctgcac gatgcggtac gtcgccacct
     1801 cgacagctgc aggcaggggc ggcaattcct ccggtgcctc gacgtagaat gccgtgtcat
     1861 cctccctgag cttctcggaa tcgtcggcga ggtatccgta gcccgccgcc tgctcgcgga
     1921 ttgccagcac tagcccgagc tcgtccaggg ccggtggccg cagcccgtag accagccgcc
     1981 tcacgtcggc tacggcctcc tgggtccgcg actttagttc tgccaagagg tgctcggctt
     2041 caccgggttc gctcctgagc gtcttgagtg agatatcaag cccgagggag agacctgcga
     2101 gcgtcggtcc aaggccgtcg tgcagatccc gcctcagtcg tctgcgctcc tcttccctgg
     2161 cggtgacgag ccgctccctg gatctctgca gatcggccgt caggcgcacg gcgtggacgg
     2221 ctacctcggc ctgcctggca aggtccctga gcaggcgcat gtcggccggc gtgaaagcgt
     2281 cgccaggctc cctcggggcg agcgtcaact ctccgatggg cctaccgccg taggtgagcg
     2341 gcaaagcggt ctcctcgccc gtgggggtgc ctttggccgc ggctacctcg tacccgctct
     2401 cgcgcgccat ggagatcgcc gcatagggta gtttgagcgc tgccgccacc gtctcgacga
     2461 tggtgggaag gacggaggcg ggttccagcg tcgcctccag gcgctcgccc aggcgtgaga
     2521 ggaccctata cggttcgtcg cgctcgccgt acatcaggcg gttcacgcca ctctgcaacc
     2581 tctggcgcag cggcgcgaac aggatcgcaa ccactcccgc cgcagggagc gagatggcca
     2641 ggctgccccg cgcctgtaga atcgccccga gggaaccgac gatgagcaca tagatgcctg
     2701 cgacgctcgc agtcagggca ccgtagacaa gcgtgcggtt tatgacgacg tccacgtccc
     2761 acagccggta gcgcaggatc gccacgccta tagacaacgg gatcagaagc agcacaaggt
     2821 tcgtggccgt caactcgaac agcgcgtaca ttatcttcgg tgcgccaggc tgcaggagct
     2881 ctggatagaa cacggcaggc aggattacca gcacggaaag tattatcgcg gacgtcaggc
     2941 cgaggaccac ccacttggtc tgctggcgtt cgaccggccc tgaaacccta cggtaacgat
     3001 aggcttgcgc gaacaccagc gcaccgagca ggctaattac gatcggggcg ctgagtagcg
     3061 acgaccacgc tcctggatag ggtgatctgt cgggcaagaa atacagtgac gcgaggtaaa
     3121 gggtccacac tatgacgagc caccgggtcc agccaggtat aaaacgtcca tccgggaaga
     3181 gatagaaggc gactacgaac gcaacgtacc cgagaaagct caagacagcg tccactcttg
     3241 ccagcaacgg gaccgtcccc gacgccagtt cgatcatgtt cagcgcgccg aatgtcacca
     3301 gcatgacaga aacaaataag gctacccaat tatccgactt cctccagaag atgaccgcac
     3361 cgatcgccca gaatcccaac ccgtacaaga tgtcagccgt gagcctgtag acggcgtagg
     3421 aactgaccga gatccccaag tcgtggagcg cacgcagatc ctccggcgcg atgcgccaat
     3481 aggggcggca ctctgctccg gcagcgcaca cgctgctgta ttcggagtag gcgccgggca
     3541 ccgaagcagc cgacagcccc acgatcagga cggtgagtac gatccatgcc gagcgcgcgg
     3601 cgagcagcgg acgcccatgt agctcagtcc gcggcggggc gggatgcacc gacgttccag
     3661 gcagggtaga ccatcggttc acgacgttct cactcttgat tgctgcatgt gcggatccag
     3721 aagccgtctc gggctatgct atgcccgatt acaggatgaa cgcgcggttg tggcgacatt
     3781 catccgtcgg ggcgcgtcag accatgcgga ccggtcggat cttctggaaa gcgctcacca
     3841 tccggcacgc acgcccgagg aattatcaag cagatcaacc ttcctgctag caccgcaggg
     3901 tccgagatgt cgaggcccac cctttccagt atcccttccc tgggaacctg ccgctggatt
     3961 ccgtcagggt gcgccgtgaa ccggagccag gtctcctcga tcctacctct tccgacgcgg
     4021 gagatggcga ttcccgacac cctcaccatg tcaccggctt cggctgctat ctcttcccgc
     4081 acgcttccgc ttcccgccca ccggaccacc accctgtcct cttctgctat ctcgtcctgg
     4141 acgttgatgc ggaagtccgg cgagaccgaa cggaacaggc tgacgagggc cttcatggca
     4201 tcccggcctt caccttcggt tcccagacca gggtaacgaa gggcatggtc ctgggcgaaa
     4261 agcttgtcga ctagctcaag atcgccgctg tcgaatgcct gctcccagaa acgcctgacc
     4321 accgccttat tctcctcaat agtcatgcat gtgctcctca ctcccggaca cgcgactgcc
     4381 gtatttcaga acttacctta caaggaaccc aagaccagcg caacggtttc cggaagcctc
     4441 ccgcaaagcc ggcgtcctcg ggcgtagtca gggagcgacc gccccgttac ggttgccgga
     4501 aaatgcctcc acaaggaccg ggaggttcga cgctaccgtg tccctatttc ggggacgcgc
     4561 cgtcccgagt gcagggacat taattccctt acgaatcggg acttcgcgct catgacagcg
     4621 ggattcctcc ccacctaact tgccattacg acgttacgga tagaggaaag gagagcagat
     4681 gggctagacg cagggacagg aactacagat caagaccata cagcaaagga gatcagttaa
     4741 ccatgttgac cagaggat
//
LOCUS       DATAIR010000619         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_135385, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000619 DATAIR010000000
VERSION     DATAIR010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135385"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHM69_14675"
     CDS             <1..315
                     /locus_tag="VHM69_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741686.1"
                     /translation="ETAFAALYTDLVLSGRLSLARLVEAMSCAPGRWVGEGGSLAVGA
                     PADLTLIDLTEEWTVGRETLISRSSNSPYLGRTLTGRVVGTIVGGEPVHDRSGAKFGL
                     RA"
     gene            308..1405
                     /gene="carA"
                     /locus_tag="VHM69_14680"
     CDS             308..1405
                     /gene="carA"
                     /locus_tag="VHM69_14680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX2741687.1"
                     /translation="MRSRATLVLEDGAAFEGWSFAGEGEVAGEVVFTTSMVGYQETIT
                     DPSYRGQILLFTYPLIGNYGVIPGDDESRRVQAAAVLVREYTPHPSNWASERSLAALM
                     DEEGVLGVEGIDTRALTRHLRDKGAMRGVISTSESDRTALREKATAHPSMVGLDLAST
                     SSELAEPTLLPAFGEERCRVVALDYGVKGSIYKELRSRGASVLAMPGSTSSEEILATD
                     PDGLFLSNGPGDPAALGEAVNTLRPLLGEVPTFGICLGHQLLGLALGCETYKMPFGHH
                     GANHPVRNLRTGRIEITSQNHGFAVSEESLSDGVELTHRNLYDGTVEGIASPDLRAWS
                     VQYHPESSPGPRDSGYLFEEFVESVSEKAPA"
     gene            1407..>2410
                     /gene="carB"
                     /locus_tag="VHM69_14685"
     CDS             1407..>2410
                     /gene="carB"
                     /locus_tag="VHM69_14685"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2741688.1"
                     /translation="MPRRDDISSVLIIGSGPIVIGQAAEFDYSGTQACRALRDEGYKV
                     ILVNSNPATIMTDPEVADATYVEPLTVETVAEILRRERPDALLPTLGGQTALNLAVEL
                     HEAGVLDELDVELLGASIPSIRMAEDRQLFSEAMRRIGLKVPDSRTVRSLAEAEELAE
                     RIGFPLIIRPSFTLGGKGGATARDNRELKRAVLDGLDASPVNSALVERSVAGWKEFEL
                     EVMRDLADNVVVICSIENVDPMGVHTGDSITVAPAQTLSDRQYQTLRTASLRIIREIG
                     VSTGGSNIQFAVDPESDEYYVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDE
                     "
BASE COUNT          465 a          769 c          767 g          409 t
ORIGIN      
        1 gagacggcgt tcgccgcact gtacacggat cttgtcctca gcggcaggct atcgctggcg
       61 cggctagtcg aagcaatgag ttgcgcccca ggcaggtggg tcggtgaggg agggagcctg
      121 gccgtcggcg cgccggcaga cctgacactg atcgacctca ccgaagagtg gaccgtcggg
      181 agggaaacgc tcatcagtcg ctcgtccaac tctccatatc tcgggcggac gctgacgggc
      241 agggtcgtgg gtactatagt tggtggcgag ccggtacacg acaggagtgg agcgaagttt
      301 ggattacgtg cgtagcaggg cgacgctcgt cctcgaggac ggggccgcgt tcgagggctg
      361 gagcttcgcc ggtgaaggcg aggtcgcggg cgaggtcgtc ttcacgacga gcatggtcgg
      421 ataccaggag accatcacag atccttctta ccgcggtcag atcctgctct tcacctaccc
      481 attgatcggg aactacggtg tgatccccgg cgacgacgag tcccgcaggg tccaggccgc
      541 cgccgtcctc gtccgcgagt acacgccaca cccgagcaac tgggctagcg agcgttccct
      601 ggcagccctg atggacgagg agggtgtgct cggtgtggaa ggtatcgaca cccgcgccct
      661 gacgcgtcac ctcagggaca agggtgcgat gcgcggtgtg atctcgacct cagagtcaga
      721 ccgcaccgcc cttcgcgaga aagcgacggc acacccctcg atggtcggcc ttgacctggc
      781 ctccacaagc tcggaactcg cggagcccac gctgcttccg gccttcgggg aggaacgctg
      841 ccgtgtcgta gccctcgact acggggtgaa gggttcgatc tataaggaac ttcgtagccg
      901 gggcgcctcc gtcctcgcga tgccgggatc gacgagctca gaagagatac tcgcgaccga
      961 cccggacggc ctcttcctct cgaatggccc tggcgacccg gccgctctgg gggaggctgt
     1021 gaacaccctg cgtccgctgc tcggcgaggt cccgaccttc ggcatctgtc tcggccacca
     1081 gttactcggg ctcgccctcg gctgtgagac gtacaagatg cccttcggcc accacggcgc
     1141 gaaccatcct gtaaggaatc tcagaaccgg gaggatagaa atcacgagcc agaaccacgg
     1201 cttcgccgtc agcgaggagt cgctttcgga tggcgtcgag ctgacccatc gcaacctgta
     1261 cgacggtaca gtcgaaggca tcgcgagccc ggacctcagg gcatggagcg ttcagtacca
     1321 tcccgaatcg agtcctggtc cgagagactc aggctatctc ttcgaggagt tcgtcgagag
     1381 cgtctcggag aaggcgccgg cctagcgtgc cgcgtcgcga cgacatctcg agcgttctga
     1441 tcatcggctc cggtccgatc gtgatcgggc aggccgctga gttcgattac tcgggcaccc
     1501 aggcatgccg cgccctgcgc gacgaaggct acaaagtcat cctcgtcaat tcgaacccag
     1561 ccaccataat gacagacccc gaggtcgcag acgccaccta cgtcgagcct cttacggtcg
     1621 agacagtggc ggagatcctg cgccgcgagc gaccagacgc cctgcttcca accctgggcg
     1681 gccagacagc cctgaacctg gccgtcgaac ttcacgaagc aggcgtcctc gacgaacttg
     1741 acgttgagct tctcggggcg tctattcctt cgatccgtat ggcggaagac cgccagctct
     1801 tctccgaggc catgcggcgc atcggcctga aggtgcctga tagccgaact gtccgcagcc
     1861 tcgccgaggc agaggagctc gccgaaagga taggcttccc gctcatcatc cgcccgagct
     1921 tcaccctcgg aggcaagggc ggggccacag cccgagacaa ccgcgagctc aagcgcgccg
     1981 tcctcgatgg cctcgacgcg agccccgtaa actcagccct cgtcgaaagg agcgtcgcag
     2041 gctggaagga gttcgagctc gaagtaatgc gcgacctcgc agacaacgtc gtcgttatct
     2101 gctccataga gaacgtcgac cccatgggcg tccacacagg cgactccatc accgtagccc
     2161 cggcccagac cctctcagac cgccagtacc agactctccg tacagcttct ttacgcataa
     2221 tacgagagat cggggtttcg acggggggat cgaacatcca gtttgcggtg gatcccgaga
     2281 gtgacgagta ctacgtgatc gagatgaacc cgagggtgag caggtcgagt gcgttggcga
     2341 gcaaggcgac ggggttcccg atcgcgaaga tagcggccaa gctcgcggtc gggtactcgc
     2401 tcgatgagat
//
LOCUS       DATAIR010000620         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_42731, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000620 DATAIR010000000
VERSION     DATAIR010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42731"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1064)
                     /locus_tag="VHM69_14690"
     CDS             complement(189..1064)
                     /locus_tag="VHM69_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX2741689.1"
                     /translation="METMDTVIGRRTERPAETPDLIPGYFARIDKGNLLTHREEIDLS
                     KRAKAGDDRARKKLVEKNLRLVVSVAKRYRGMGLPFEDLIQEGNVGLMRAVEKFDPDR
                     GWRFSTYATWWIRQAVQRAVADKGRTIRVPVHMGDKIRKMARAYNELSAELTREPTDE
                     EVAGRLEWTVDEVLDVKSAMPDATSLNQTLSADGDGSELGELIEDERASEVAGVVIGE
                     IEVGSLKEAVQKLPERHRHVLIRRYGLGNRDTATLAQLSDELGISRERVRQLQREAER
                     MLGSGELGMVLRGAA"
     gene            1407..>1532
                     /locus_tag="VHM69_14695"
     CDS             1407..>1532
                     /locus_tag="VHM69_14695"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2741690.1"
                     /translation="MSVATVTEISATSPDGFDAAVKEGIARANKTLRNVESAWVKD"
BASE COUNT          288 a          495 c          462 g          287 t
ORIGIN      
        1 gggcgtctta gaacgaagtc gcacaggatc atccgcgacg aaagctccgg gctctttccg
       61 agcgaccact acccgatact cgcggaactc gtaccggagg aagggcaaag ccgttaggcg
      121 aacagggtct tgcacaaacg aaaagagccc cgcgaacggg gctcctctcg taaagaaaat
      181 cctactcctc aggcggcgcc gcggaggacc atcccgagct caccgctacc gagcatacgc
      241 tcggcctcgc gctgcagctg gcggactcgc tcccgcgaga tccccagctc gtcgctgagc
      301 tgggccaggg tcgcggtgtc gcggttgccg aggccgtaac gccgaatcag gacgtggcgg
      361 tgacgctccg ggagcttctg tactgcctcc ttcagcgagc caacctcaat ctcgccgatg
      421 accacgcctg ccacctccga ggcgcgctcg tcctcgatca gctcgccgag ctcggatccg
      481 tcgccgtcgg cggagagggt ctggttgagg ctcgtggcgt cgggcatagc gctcttgacg
      541 tcgagcacct cgtcgaccgt ccactcgagg cgtccggcga cctcctcgtc ggtgggctcg
      601 cgcgtaagct cggcggagag ctcattgtac gcgcgggcca tcttccggat cttgtccccc
      661 atgtgcacgg ggacgcggat ggtacggccc ttgtcagcga cggcccgctg cacggcctgg
      721 cggatccacc acgtcgcgta cgtggagaac ctccagccgc ggtcagggtc gaacttctcg
      781 accgcacgca tgagaccgac gttgccctcc tggatgaggt cctcgaaggg aagccccatc
      841 ccacggtacc tcttggccac ggatacgacg agcctgaggt tcttctcgac cagcttcttc
      901 ctggccctat cgtcgcccgc cttcgcccgc ttgctgaggt cgatctcctc ccggtgagtc
      961 aggaggttcc ccttgtcgat gcgcgcgaag tagccgggga tgaggtcagg ggtctccgca
     1021 ggccgctcag tccgccgccc gattactgta tccatggtct ccatcaagct cctctctatc
     1081 ctgtaataaa ccgtcaacgt cctctaaaac ccgcactact ttcccacttc caggcgccag
     1141 aaacccagag taataccgcg cgggcatcgc cctccggtga cgcgaagttg tatggatgcg
     1201 ggaatgcagg ctttttatag aattacgccg aactagtagt ttacacattc aagagcgttt
     1261 gtcaagggcg atttcgaccg ccgagacctg cgtagatact gctcatcgcg ggctctggaa
     1321 gggcgccggc gtttagcctc gcgccgtggc gggaagttta actgtagata gggaagaagt
     1381 acaggctagt aggatggagg cacgatatga gcgttgccac cgtcaccgag attagcgcca
     1441 cctctcccga cgggttcgat gccgctgtca aagagggaat agcgcgtgcc aacaagaccc
     1501 tgcgcaacgt agagagcgct tgggtcaagg ac
//
LOCUS       DATAIR010000621         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_412708, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000621 DATAIR010000000
VERSION     DATAIR010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412708"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VHM69_14700"
     CDS             complement(<1..244)
                     /locus_tag="VHM69_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2741691.1"
                     /translation="MDSSAATEVIARLKAEYPDARTELNWTNPLELLVATILSAQTTD
                     VRVNQVTESLFAKYRTAKDYADSAPDELEEDIRPTGF"
     gene            315..1613
                     /locus_tag="VHM69_14705"
     CDS             315..1613
                     /locus_tag="VHM69_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX2741692.1"
                     /translation="MAEITFDYQNLMEVEGGLQSGELEELRPRLREAADDLLASPPGF
                     MRLPKTAEYAEGSARIAQEIQASGATDFIHVGIGGSALGPMAVHKALNHPYYNVLPGE
                     ARRGPRLHFAENTDPATLSAILDVADPDGTWVNVVTKSGSTAETMANFLVIRGFLMDS
                     LGDFGFQERTIATTDPEEGFLKQIADKEDLKVLPIPPDVGGRFSVLTPVGLFPAAVTG
                     LDIDALLSGAAQCVDDMNEQGAEHPAAVGAAMHYLMDTARGRNVRVMMTYADALERLA
                     AWFVQLWAESLGKDGKGSTPHGAVGTTDQHSQVQLYMEGPQDKVIEIVEVGNHQRDLE
                     IPGAYEDLEGVGYLGGHTMAELLNVECDATRRALTEAGHPNSTIKLGTVNEENLGYLL
                     QALEVQTAIAGALYGVNAFNQPGVEAGKQITYKWMGRPGY"
     gene            complement(1740..2561)
                     /locus_tag="VHM69_14710"
     CDS             complement(1740..2561)
                     /locus_tag="VHM69_14710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741693.1"
                     /translation="MRGIQPLINLGLLLSLAPRRPREFRDRVAGYADLGLERFSGVAP
                     EYETVGWEEALRDVEKHLGRVAEILDEPALKETEENTRRLLEDIRGEDAFSPRWAADS
                     RLARLCYLLCRLIRPSIVIETGVAYGVSSAFILRAMDENGIGTLHSVDLPPLRKEYER
                     FWGVAVTETLKGRWKLHRGSSVRNLPRLLDEVRAIDLFVHDSLHTQRNMRREFDTVWP
                     RLRNGGALLADDVERNRAFGELQRRSPALWRVVRDREEAPLHGTAAPVVFGIAVK"
     gene            2686..3297
                     /locus_tag="VHM69_14715"
     CDS             2686..3297
                     /locus_tag="VHM69_14715"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX2741694.1"
                     /translation="MKWLQVYGLPKLPLFLARWAMRLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMSRIGEKQGSDLREQLGVKTMKDTVDVVMLANRFFDI
                     EITLTETKDGEYVINADRCPWFGGMGHDGVPGWDVKPCAAFSTYEKAMVNAINPNVKL
                     SYTEKRTTGGHTCRGVYQYKDKSLGDNVESQTEFVGLGKKPGK"
     gene            complement(3333..>3852)
                     /locus_tag="VHM69_14720"
     CDS             complement(3333..>3852)
                     /locus_tag="VHM69_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2741695.1"
                     /translation="KEPAYGYALGMVPGVDLRVLHLIRDPRAAAYSWAKKKRQPDSTD
                     REFMHRKSPSQSAVLWDAWNAAIEVLWRETPEKYLRLRYEDFVSDPRRSFEDILKLTG
                     EENADLPLVGEREVKLGISHTVSGNPNRFDTGAVELRQDSEWQKKMKPRDNALVTALT
                     LPLLTRYRYTLS"
BASE COUNT          750 a         1180 c         1238 g          684 t
ORIGIN      
        1 agaagccggt cgggcggatg tcctcctcga gctcgtcggg ggcggaatcc gcgtagtcct
       61 tcgccgtacg gtacttcgca aagaggcttt ccgtcacctg attcacccgt acgtcggtgg
      121 tctgggcgga gaggatcgtt gctaccagga gttccagggg gttcgtccag ttcagttcgg
      181 tgcgcgcgtc gggatactcc gccttcaggc gggctatgac ctcggttgct gccgatgagt
      241 ccacgatggc cggagattat accctgttct aaactatctc cgtaacggag actgcgaccg
      301 atgggagcct gagcatggcc gagatcacct tcgactacca gaacctcatg gaggttgagg
      361 gtggtctgca gagcggggag ctggaggagt taagaccccg cttgcgcgag gctgcggacg
      421 atcttctcgc gagcccgccg ggcttcatgc ggttgcccaa gaccgccgag tacgcggagg
      481 gttcggcgcg tattgcccag gagattcagg cctccggggc gacggacttc atccacgtag
      541 gcatcggagg ctctgctctg ggcccgatgg ccgtgcacaa ggcgctgaac catccttact
      601 acaacgtgct gccaggagaa gcccgtcgcg gtccgcgcct ccacttcgcg gagaacacag
      661 accctgcgac gctctccgcc atactcgacg tagctgatcc tgacggaacg tgggtcaacg
      721 tcgtgaccaa gtcaggctcg acggcggaga cgatggcgaa cttcctcgtg atccggggct
      781 tcctcatgga ctctctgggg gacttcggct tccaggaacg cacgatcgca acgaccgacc
      841 cagaggaagg tttcctgaag cagatcgccg acaaagaaga cctcaaagtt ctccccatac
      901 caccggacgt tggcggcagg ttctctgtcc tcacaccggt cgggcttttc cccgccgccg
      961 tgaccggtct cgacattgac gcgctgctct ccggggccgc ccagtgcgtg gacgatatga
     1021 acgaacaggg ggcggagcat cccgcggccg tcggggcggc gatgcactac ctcatggata
     1081 cggcgcgggg gcgcaacgtg cgggtaatga tgacctacgc cgacgccctg gagcggctcg
     1141 cggcctggtt cgtccaacta tgggccgagt cccttggcaa ggacggcaaa ggctcgacac
     1201 cccacggcgc ggtcggtacc acggaccagc actcgcaggt ccagctctac atggagggtc
     1261 cccaggacaa ggtcatcgag atcgtcgagg tcgggaacca ccagcgcgac ctggagatac
     1321 caggagcata cgaagacctc gaaggcgtag gctacctcgg gggacacacc atggccgagc
     1381 ttctcaacgt cgagtgcgac gccacccgaa gggctctcac agaagcaggg catccgaact
     1441 cgaccatcaa gctcggcacc gtaaacgagg agaacctcgg atacctgcta caggccctcg
     1501 aagtacagac cgccattgct ggcgcgcttt acggggtcaa cgccttcaac cagcccggcg
     1561 tggaggctgg gaagcagatc acctacaagt ggatgggaag gccagggtat taggcatcag
     1621 gctacaggtc tcagaaccaa aaccgtggcc gagtgcgagt ggatgaaagc tacggacgaa
     1681 cgtctaacga tggatcccta atcgctgata cctgaaacct gatgcctgta gcctgatgcc
     1741 tatttcaccg cgatcccgaa cactaccggc gcggccgtgc cgtgtagcgg cgcctcctcc
     1801 ctgtccctga ccacgcgcca taaggccggg ctccttcgct gtagttcgcc gaaagcgcgg
     1861 ttcctctcga cgtcgtcggc gaggagcgcg ccaccgtttc gtagacgggg ccataccgtg
     1921 tcgaactcgc ggcgcatgtt cctttgggtg tgaaggctgt cgtgaacgaa gaggtcaata
     1981 gcacggacct cgtcgaggag acggggcaga ttgcggacgc tggagcccct gtgcagcttc
     2041 caccggcctt tgagcgtctc ggtcacggca acgccccaga acctttcgta ctcctttctc
     2101 agaggaggca ggtccacgct gtggagcgtg ccgatgccgt tctcgtccat cgctctcaag
     2161 atgaacgccg agctcacgcc gtaggcgacc cctgtttcga tcacgatgga aggtctgatc
     2221 agtcggcaca acagatagca aagacgggcg agccgcgagt cggcggccca gcgcgggctg
     2281 aatgcgtcct cgccacggat gtcttcgagg aggcgacgcg tgttctcctc ggtctccttg
     2341 agggcaggct cgtcgaggat ctcagccaca cgaccaagat gcttctccac gtcccgaagc
     2401 gcctcttccc agcctaccgt ctcgtactcc ggcgcaacgc ctgagaaccg ctccaggccg
     2461 aggtcagcat accccgccac cctgtcgcga aactctcgcg ggcgccgcgg ggccagcgag
     2521 aggaggaggc ccagatttat caggggctgt atgccacgca cggctacgct ccctcggctc
     2581 tctacgacag gacggcctaa tggtaaggca cgggggcgcc atacggggtg gtccgggtat
     2641 aatggggcct gcgccggctc aagcgaacga agggtattct ctattatgaa gtggctacag
     2701 gtctacggcc ttcctaagct cccgttattc ctcgccaggt gggcgatgcg tctcgggctg
     2761 cccggtccga cgtggtacaa gtggaaggcc gcatcggcgg ggacgggtgt aatcctggac
     2821 gagatgatcc gggccgcaga cgacctcggc tacgacggcc agaaggtcgg taagctcgca
     2881 atgagccgga tcggcgagaa gcagggctcc gacctgcgcg agcagctcgg cgtcaagacg
     2941 atgaaggaca ccgtagacgt agtcatgctc gcgaaccgct tcttcgatat cgagatcaca
     3001 ctgacggaga ccaaagacgg cgagtacgtg ataaacgcgg accgatgccc ctggttcggc
     3061 gggatgggcc acgacggcgt ccccggctgg gacgtgaagc cctgcgccgc tttctcgacg
     3121 tacgagaagg cgatggtgaa cgccataaac ccgaacgtaa aattgtcgta caccgagaaa
     3181 cgcaccaccg gcggtcacac ctgccgcggc gtctaccagt acaaggataa gagtcttgga
     3241 gacaacgtcg agtcccagac ggagttcgtg gggctcggca agaagcccgg aaagtaggga
     3301 tgtagcgggc gacacttgcc cgccgaggct gcttaactca gcgtatagcg gtagcgtgtc
     3361 agcaggggca gagtcagtgc ggtgacgaga gcgttgtcac ggggcttcat cttcttctgc
     3421 cactcgctgt cctgacgcag ttcgaccgcg cctgtgtcga agcgattggg gttgcccgag
     3481 acggtgtggc ttataccgag cttgacctcc cgctctccta ccaggggcag atcggcgttc
     3541 tcttccccgg tgagcttcag gatatcttcg aagctgcggc gggggtccga gacgaagtcc
     3601 tcataacgga ggcggaggta cttctcgggg gtttcgcgcc agaggacctc gatggcggcg
     3661 ttccaggcgt cccagaggac agcgctctgg gacggggact tccggtgcat gaactcgcgg
     3721 tcggtgctat caggctggcg cttcttcttc gcccacgagt aagcggcggc ccttgggtcc
     3781 cggatcaggt gcagaacgcg gaggtccacg ccagggacca tgccgagcgc ataaccgtag
     3841 gccggctctt tg
//
LOCUS       DATAIR010000622         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_320243, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000622 DATAIR010000000
VERSION     DATAIR010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320243"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VHM69_14725"
     CDS             complement(<1..1347)
                     /locus_tag="VHM69_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2741696.1"
                     /translation="MIGGAQGGSDPFEMLQNLIQQQQGAQGSLFDALSGEAQQAVMRA
                     REASAGTTETAAIGTDHLLAGIVTGDNVATEALQRAGADVNGMRTRFQEFRGDPGERI
                     YTFTPSAKRALQLSFAAARQMSAGQVGSEHLLLGLLGEGDGNAYRILQQHGASDAEAL
                     RREILRLMQQRGAQTGPGAGMPGAAAGGPGDGQQGPPSRTPTLDQVSRDLTQLAREGE
                     LDPVIGRAEEISRVIRILSRRTKNNPVLIGEAGVGKTAVAEGLAQRIISEDVPETLKD
                     KRVVALDVGGLVAGTRFRGDFEERMQQMLKELQQEEKNIVLFIDELHTIVGAGGAEGA
                     VNASNMLKPALARGELQVVGATTLDEYRKHVEKDPALERRFQPVLVDEPTVDETVAIL
                     FGLRDRYEAHHRVRISDEAIIASAQLGDRYITDRFLPDKAIDLLDEAAAEVRLRSTV"
     gene            1654..2625
                     /gene="pfkA"
                     /locus_tag="VHM69_14730"
     CDS             1654..2625
                     /gene="pfkA"
                     /locus_tag="VHM69_14730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2741697.1"
                     /translation="MRIGVLTSGGDAPGMNAAVRAVARTAFFKGWEVMGIWSGYRGLL
                     EGRIYPLDRRRLGGIIHRGGTVLGTERSPEFATPEGQRKAAGKLAEAEVEGLVVIGGN
                     GSLTGGLRLAELGVKVAGVPATIDNDVWGTDTAIGVDTALNTALEAIDRLKDTASSHQ
                     RAHVVEVMGRDCGYLALMSAIAGGAEAAVVPEFEPKPEALLQFLKKAFEQGKSHFIVV
                     VAEGAPLSAEELHEYVNDSEGTYDARLTVVGHIQRGGIPTASDRILASRLGTAAVEAL
                     ADGESGVMVGLRGTRIERTPLEEVVGKEQPLDPDLYKMAEVLAELPE"
BASE COUNT          478 a          874 c          937 g          510 t
ORIGIN      
        1 caccgtcgaa cggagccgca cctcggctgc ggcctcgtcg agtaggtcga tggccttgtc
       61 gggcaggaag cgatccgtta tgtagcggtc gccgagctgg gctgaggcga tgatcgcctc
      121 gtccgagatg cgcaccctgt ggtgcgcctc gtaacggtcc ctgagaccga agaggatcgc
      181 caccgtctca tccaccgtgg gctcgtccac gagcacgggc tggaagcgcc gctccagcgc
      241 ggggtccttc tcgacgtgct tgcggtactc gtcgagcgtg gtcgccccga cgacctgaag
      301 ctcgccgcgt gcgagcgcgg gcttgagcat gttcgaagcg ttcactgcac cctcggcgcc
      361 gcctgccccg acgatggtgt ggagctcgtc gatgaacagg acgatgttct tctcctcctg
      421 ctggagttcc ttgagcatct gctgcatccg ctcctcgaag tccccgcgga agcgggtgcc
      481 agcgacgagc ccacccacgt cgagggcgac gacgcgcttg tccttaagcg tctcagggac
      541 gtcttcggag atgatcctct gcgccaatcc ttcggctacg gcagtcttgc cgacgccggc
      601 ctctcctatg agaaccggat tgttcttcgt gcggcgggag aggatgcgga tgacgcgtga
      661 gatctcctcg gccctcccga tgacggggtc gagctcgcct tcgcgcgcca gctgcgtgag
      721 atctctactg acctgatcga gggtcggcgt ccggctcggc ggcccctgct gcccgtcgcc
      781 agggcctccc gcggcggccc cgggcatccc tgcccctggt ccggtctgcg caccccgctg
      841 ctgcatgagg cgcaggatct cacgcctcag cgcctctgcg tcgctggcgc cgtgttgctg
      901 caggatcctg taagcatttc cgtcgccctc gccaaggaga ccgagcaaga gatgctcgct
      961 cccgacctgc cccgcgctca tctgcctggc ggcggcgaag gagagctgca aggcccgctt
     1021 ggcgctcggg gtgaacgtgt agatccgctc tcccggatca cccctgaatt cctggaaacg
     1081 cgtgcgcatt ccatttacgt cagcgcccgc acgctgcagg gcctcggtag ccacgttgtc
     1141 cccggtcacg atgcccgcga gcaggtggtc cgtgccgatg gcagccgtct cggtcgttcc
     1201 cgccgacgcc tcacgcgccc gcatcacggc ctgctgcgcc tccccggaga gcgcgtcgaa
     1261 gaggctaccc tgcgcccctt gctgttgctg tatgaggttc tggagcatct cgaagggatc
     1321 cgacccaccc tgcgcgcccc cgatcatgcc gagctgccgc gcgcactgcg tgcacagata
     1381 gttggcaacc ctccgatccc cctgctgctg gaagacctgt acactcgcag gccgtatacc
     1441 acagttttca catatcattt gttcaaagcc tcctgtatta aatcttcgta ctccctttct
     1501 ccctgtaccg ggagaaccat aacagtcaaa atctaagcct gtcacgctca tattttatta
     1561 ctccttcgcg gtagatcaag gcatcgagga aatcgggata ttgtgtctgg ttccggcata
     1621 atgggtctct gaggtagaga gaggagacgg atcttgagga taggggtatt gaccagtggt
     1681 ggcgacgcgc ccgggatgaa tgcggcggtg cgtgctgtgg cgcggacggc gtttttcaag
     1741 ggttgggagg tcatggggat ctggtccggc tacagggggc tccttgaagg caggatctac
     1801 cctctggacc ggcgccgtct cgggggcatc atccaccgtg gtgggaccgt gctggggacg
     1861 gaacgctcac ccgagttcgc gaccccggag ggacagagga aggcggcggg gaagctcgcc
     1921 gaggccgagg tcgaagggct cgtcgtcata ggcggtaacg ggagcctgac agggggcttg
     1981 aggctggctg agctcggggt caaggtggcg ggagttcccg cgaccataga caacgacgtg
     2041 tggggcaccg acacggccat aggggtggac actgcgctca acacggcgct ggaggcgata
     2101 gacaggctca aagacaccgc aagctcccac cagagggcgc acgtggtcga ggtgatgggg
     2161 cgcgactgcg gctacctggc gctcatgagc gccatcgcgg gaggggccga ggccgccgtg
     2221 gtccccgagt tcgagccgaa gcccgaggct ttgttgcagt tcctgaagaa agctttcgag
     2281 cagggcaagt ctcacttcat agtcgttgtc gcggaggggg ctccgctttc agcggaggag
     2341 ttgcacgagt acgtaaacga ctccgaggga acgtacgacg cccgtctcac ggtcgtaggc
     2401 cacatccagc gcggcggcat ccccaccgcc tcggacagga tactggccag ccggctcggt
     2461 acggcggcgg tcgaggcgct cgccgacggg gagtcagggg tgatggtcgg gctcaggggg
     2521 acacggatcg aacgcacacc gctcgaggaa gtcgtcggca aggagcaacc cctcgacccc
     2581 gacctctaca aaatggccga ggtcctggcc gagctgccgg agtaatggta gagggtatat
     2641 ccttaccaga cggaaactat ttcggaggtc gacgtgcaag agcgaggtct catagggctc
     2701 gaagctcggg cggcggacgg tactgaggtc ggtcgcatcg tcgacgtcgt ctcggacgag
     2761 gagagcgggg aggttacaca cgtagtcgtg gagaccgag
//
LOCUS       DATAIR010000623        10221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_412734, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000623 DATAIR010000000
VERSION     DATAIR010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412734"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..221)
                     /locus_tag="VHM69_14735"
     CDS             complement(6..221)
                     /locus_tag="VHM69_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741698.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDRYLAEEAALRNLQSDKQTI
                     HPRDLPPDKREALIEEVFEILKDTRTR"
     gene            complement(283..1185)
                     /locus_tag="VHM69_14740"
     CDS             complement(283..1185)
                     /locus_tag="VHM69_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2741699.1"
                     /translation="MSPGRVRVEFWIVVTLAHISDLHFGRPAVVERLEALQRYIAELQ
                     PEAVAVSGDLTQRCTRREFSNASAYLDSIGEMAPCVVVPGNHDIRWLGAVARNLGLAG
                     LFRKQAHKFKYSRYTKYVSEDLSPSLEIQGAVIAGINTAHGITRGSITRRFRDLGVIG
                     HVKHKDVTRVREAFENAAPDAARIVLMHHNPIKGELSGRHGLANTEQALHAFSDLGTE
                     LILCGHDHLDAVHTIERGAHGLIISTAGTISSRIRPGRASSFNVVEIKDHEFRITAHT
                     WRQGKGFLPAEVCTFPRRTTSKQA"
     gene            1484..2929
                     /locus_tag="VHM69_14745"
     CDS             1484..2929
                     /locus_tag="VHM69_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741700.1"
                     /translation="MAVLLMAASGPARAAFPGTSGKIAFYTGGDVWTMNGDGTNPTKL
                     TSNYNAEANPAVSPDGSRIAYEFLRGIWMMNSDGSGKRALTDGTATDEDPAFSADGTR
                     VAFARNGDIWAMNVDGSGVTNLTNSPNNQELDPAWSPLGDKIAYTRIGCDPANFGAVC
                     VYSMNADGKGQTNLTPETSMPQCPNQPGYFHNGASREPAWSPDGTLIAFSGALICPHT
                     TGRDIWVMSATGGAKTNLTNDNGTNDKRPAFSPDGTKILFESDRESTNLELYTMSTNG
                     SGLQRLTNNGTLDDNPDWGVARPECATTDGATNPSLLGTPSADSITGTPGNDIICGLG
                     GNDTVDGGGGNDIILGDAGDDRLTGASGRDTLNGGAGTDTASFAGSSQAVEASLMSGF
                     ARRAGTSPVEGVALVGIENLSGTSLSDMLSGSAGANTLVGGQGADELLGLGGNDELKS
                     RDGARNDSLNGGPGTNRCTTDNREVSIRNCS"
     gene            3038..3862
                     /locus_tag="VHM69_14750"
     CDS             3038..3862
                     /locus_tag="VHM69_14750"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HEX2741701.1"
                     /translation="MLFILIFGGFGILMWVASGVLLYQRRKQLRKTDLMRRVETSQAS
                     QVSALSAGTPVEVKGFVRCEEPLKSEMAGHECAYYLSQVIREYHETDRDADGDMQTRR
                     RSEVMASNERFAPFVVEDDSGVVGIRGEGAEVDALEVMNRFENDTGGEGSITVGGISV
                     NLGGGARTIGYRYVETVLPVDAPVYVLGVAQEDGHIGSPVEEGGEERFLISHRSEEQL
                     EKKYRRSALLLGLLAVGLFLFGAIFVVAGVVAGVFSMGASAAVEPSVAVAERILSG"
     gene            3859..4320
                     /locus_tag="VHM69_14755"
     CDS             3859..4320
                     /locus_tag="VHM69_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741702.1"
                     /translation="MNGRPGRMRSRLTVATLFAGCCALVLTACTSGTITGSEQACKST
                     GGLFADKQVACTGSVSGVRGEPSLGIIDTDEDLSGDYKLDATFTVEKGTAYVSTAEGG
                     QAGGKVTPDEPLRINTVVALDESDEDVSVNLKVPGKEVKDLRYEATLLPQD"
     gene            complement(4330..5127)
                     /locus_tag="VHM69_14760"
     CDS             complement(4330..5127)
                     /locus_tag="VHM69_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2741703.1"
                     /translation="MDHRPSGVAMSYRDLGDGDGVPLVLLHAFPLNARMFEPQMTAFS
                     GERRVVAPDYPGFGRSPRTPAQPDVHYYAEGVRRLLDRLHLERVVLGGVSMGGYVAFE
                     CMRVFPERISALVLANTRPEPDSEEMRETRNEMALRVAQEGVGVLVKLQLERLLAPYT
                     LQNDEALVEKVRTMILENSPDGAVAALGAMRDRPDSTPLLGEIGVPTLVVGGEEDGIS
                     SPEVMSAMAAKIPGAHHVTIPNAGHLSNLEAPGEFNAALNDFLKSIK"
     gene            complement(5286..6767)
                     /locus_tag="VHM69_14765"
     CDS             complement(5286..6767)
                     /locus_tag="VHM69_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2741704.1"
                     /translation="MAKKAAGVPVWISLDFSSDAPIYRQLYERMRTEILGGRLPASTR
                     LPSTRTLATELGVSRSTVVTAFEHLLAEGYLEGKVGSGTYVARSLPEEMLGVQGRRIH
                     EQGPLRSGRGLSRRGTLLAATPTTIAWDMGTPRAFRPGVPALDQFPYGVWRRISGSVW
                     RRPSGAMLGYGDPAGYWPLREEISAHLRAARAVRCEPEQVIVVSGSQQALDLASRVLL
                     DPGDAVWVEDPGYVGARGALSGSGARLVPVPVDEEGLIVTTGIEREPGARLVCITPSH
                     QYPLGVTMSLARRLELLRWASSSGAWVIEDDYDSEYRYTGRPLEALQGLDTEGCVVYV
                     GTFSKVLFPALRLGYLVVPPDLVDAFVAARELTDRHPPIVEQTILAEFIAGGHFVRHL
                     RRMRALYAARQAVLVEEISHELRGLLDSSPASSGMHLVGWLSEGIDDREASRLAATQG
                     VEAPPVSLYKTRPDGQGGLMLGYAAVDEAGIRDGVRRLANALQ"
     gene            6906..7499
                     /locus_tag="VHM69_14770"
     CDS             6906..7499
                     /locus_tag="VHM69_14770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2741705.1"
                     /translation="MWKNLTSVLEGGIVRLEPLARGHEQGLFEVARDERIWRWMPYDA
                     GKSSETFNTWLEDAVAASEAGSEGAFVTLDARTGEPIGSTRYLALRAEHRGLEIGWTW
                     LAPSRWQSGANVEAKLLMLEHAFERLGCLRVEFKTDARNERSRAALAALPAQFEGVFR
                     KHMLVRDGERRDSAYYSIIDDEWHEVRDNLRRRLAVV"
     gene            7529..7771
                     /locus_tag="VHM69_14775"
     CDS             7529..7771
                     /locus_tag="VHM69_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741706.1"
                     /translation="MARIEGVDPERVEKRIRAVLEAQADRWGAPLLNHLIYARRPSIY
                     RGVRGMWAGLAVSGLLDSGLVALVNRRVAALNGCDF"
     gene            7790..8092
                     /locus_tag="VHM69_14780"
     CDS             7790..8092
                     /locus_tag="VHM69_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741707.1"
                     /translation="MGSELGISDEKILALEEYATSPLYDDQERVALEYADAMTLSDSD
                     VEEELFERVRAVFDEDAVVELTAVISWENSSSKFNRALRVPSQGLWKREHGAGGSE"
     gene            8089..8445
                     /locus_tag="VHM69_14785"
     CDS             8089..8445
                     /locus_tag="VHM69_14785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2741708.1"
                     /translation="MSGEVRRGAGTRRATVAEAMGRLPGSGGERFAKVLEHGSMEVEI
                     YAPRDSDPQTPHTRDELYVVVGGSGEFVNGRERNSFGPGDVLFVPAGVEHRFEDFTDD
                     LVTWVIFYGPEGGEAR"
     gene            8442..9056
                     /locus_tag="VHM69_14790"
     CDS             8442..9056
                     /locus_tag="VHM69_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX2741709.1"
                     /translation="MTLRLHDNLSSGNGYKVRLLLTQLGIPFERIEYDITTGGTRTPE
                     FLEKVNANGRIPVLETEEGEFLPESNAILFYLADGTPLLPDDRLGRAMALQWMFFEQY
                     SHEPNIAVARAWLHVFDVRMTEERLGVLETKRKLGYDALGVMEKHLKVNDFFVGDYSI
                     ADIALYAYTHVAGEGGFDLDGFPAVLAWLERVASQPGHIPITQG"
     gene            9081..10169
                     /locus_tag="VHM69_14795"
     CDS             9081..10169
                     /locus_tag="VHM69_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2741710.1"
                     /translation="MQTEPRNSHPFHMHDAILAQPEAFVRAVERNEDALDEFASRIAS
                     CERIFLSGIGTSHHASLVGECLMRAYGGGPDVRAFHSFDLALYGPQMRPEDCVVAVSH
                     RGAKRYTALALDRAREMGCMTALVTGEAGSGEGRADALFRTVAQERSPAHTVSYTGAV
                     AVLALLAGRLGYHRTGSATLPDELLRDEVPGALRAALGTEEEVAALAREHVGRRRIWL
                     VGGGPAAVTAMETALKIKETSYLQAEGMSTETMLHGPFQCVEEDDLFVLIAPSGAARE
                     RTLEVAELAEMVGATCLILGDDTTEAPLDDVSILSVPEVPEPFSALSCIVPLQLFAYH
                     LALARGTNPDSFRGDDPRFARADVSGRL"
BASE COUNT         1911 a         3123 c         3379 g         1808 t
ORIGIN      
        1 agtcgctagc gggtgcgggt gtccttcagg atctcgaaga cctcttcgat cagggcctcg
       61 cgcttgtccg gagggaggtc gcgaggatgg atagtctgtt tatctgactg gaggttgcgc
      121 agggccgcct cctcggccag gtaccggtct ataaggcgga cgatggcgtc caccttctca
      181 cggggcagtc cgtcgccgcc gcgggtcagt ctgctccaca tgtcgatagt cccttcagtg
      241 acggttggcg gtgtcgcggc agattatacc gtcgggtgtg cttcaagcct gcttcgaggt
      301 cgtgcggcgt gggaacgtgc agacttccgc gggtaagaag ccctttccct gacgccaggt
      361 gtgggccgtt atacggaact cgtgatcctt tatctccacg acgttgaagc tcgatgcgcg
      421 tccgggacgg atgcggctcg agatcgttcc ggcggtcgag atgatgaggc cgtgggcgcc
      481 ccgctcgatc gtgtggacgg cgtccaggtg gtcgtgtccg cagaggatca actccgtacc
      541 caggtctgag aaggcgtgca gggcctgctc cgtgttggcc aggccgtggc ggccggagag
      601 ctcgcccttt atcgggttgt ggtgcataag cacgatgcgg gcggcgtcgg gggcggcgtt
      661 ctcgaaggcc tccctcacgc gcgtcacgtc tttgtgtttg acgtggccaa taacgccaag
      721 gtcacggaag cggcgggtga tcgaaccccg cgtgatgccg tgggccgtgt ttatccccgc
      781 gatgacggcg ccttgaatct ccagagaagg gctcaggtct tccgagacgt attttgtgta
      841 gcgggagtat ttgaacttgt gggcctgctt cctgaagaga ccggcgaggc cgaggttgcg
      901 ggcgacggcc ccgagccagc gaatatcgtg attgcccggg acgacgacgc aaggcgccat
      961 ctcaccgatc gagtcaaggt aagcgctggc gttcgagaac tccctcctcg tacagcgttg
     1021 cgtcaggtcg ccagagacgg ctaccgcctc aggctgtagc tctgcaatgt acctttgcag
     1081 agcttcgagg cgttcgacga cagcgggacg cccgaagtgc aaatccgaaa tatgcgcaag
     1141 ggtgaccacg atccagaatt ctacccgaac gcgccccggc gacaaagtcc gccctccgga
     1201 gatccaaccc gacggaaccc cgcgacttcc tcccccacag ttcgagcgtg ggggtggtgt
     1261 gggggtggtg tgggtggttc gcgaacctgc gcgccggcag agtacgagtc aggaaagtcc
     1321 ggaccgaaag tgaggtggcc tgacaacggg agaccgcgaa cggccacagg aagtgcaagc
     1381 acgaacgatg agaggagcaa agatgacaaa gcagacctcc atgaaggtaa tgaagatcga
     1441 cagagcgatg ttcttctagg tgggcctggc tgtggtgctg tcgatggccg tgctcttaat
     1501 ggcggcctcg ggacccgcgc gggcggcctt ccccggcacg agcggcaaga tcgccttcta
     1561 caccggcggc gacgtgtgga ctatgaatgg ggacggcacg aacccgacaa agctgaccag
     1621 taattacaac gccgaggcaa accccgccgt ctcgcctgac ggctctcgca tagcctacga
     1681 gttcctgcgc ggcatctgga tgatgaactc cgacggctcg gggaagaggg cgctcacaga
     1741 cggtacggcg accgacgagg acccggcctt ctccgctgac ggaaccaggg tggccttcgc
     1801 gaggaacgga gacatctggg cgatgaacgt ggacggttcc ggcgtgacca acctcaccaa
     1861 cagcccgaac aaccaggagt tggatccggc gtggtcgcca ctgggagaca agatcgccta
     1921 cacgcgcata ggctgcgacc cggccaactt cggggccgtc tgcgtctact ccatgaacgc
     1981 ggacggtaag gggcagacca acctcacccc cgagacctcg atgccgcagt gccctaacca
     2041 gccaggctac ttccacaacg gcgcgagccg tgagcccgca tggtctcctg atggaaccct
     2101 gatcgccttc tcaggcgccc tgatctgccc gcacaccacg ggcagggata tatgggtgat
     2161 gagcgcgacc ggcggagcga agaccaacct cacgaacgac aacgggacga acgacaagcg
     2221 gcccgctttc tcgcctgacg gcacgaagat cctcttcgag agcgatcgtg agagcaccaa
     2281 tctggaactg tacacgatga gcacgaacgg ctcggggctg cagcggctaa ccaacaacgg
     2341 cacccttgac gataatcctg actggggagt agcaaggccg gaatgcgcca cgaccgacgg
     2401 tgctaccaac ccgagcctcc tcggcacccc atcagccgat tccatcactg gcacgccggg
     2461 caacgacatc atatgcggcc tcgggggcaa cgacactgtc gatggcggcg gtggtaacga
     2521 catcatcctc ggggacgcgg gtgatgacag gctcaccggt gccagcggca gggacacact
     2581 gaacggcggc gctggtacgg acacagcctc tttcgcaggc tcgtcccagg ccgtggaggc
     2641 ctcactgatg agtggtttcg caaggagggc gggcacgagc cccgtagaag gggtggctct
     2701 agtcgggatc gagaacctga gcggcaccag cctctctgat atgttgagtg ggtccgccgg
     2761 ggcgaacacc ctggtcggcg ggcagggtgc agacgagcta ctcggcctcg gcggcaacga
     2821 cgagctcaag tcgcgggatg gtgcgcgcaa cgactccctc aacgggggac ccggcaccaa
     2881 ccgttgcacg acggacaaca gggaggtttc gatcaggaac tgttcctagg aactgctcct
     2941 gggagacgag gctggggagg agtgccgccg cgctcctccc cgagcgagtc caagcccgat
     3001 gccgcatact acggacgagc gacggggagg aatctttatg ctgttcattt tgatcttcgg
     3061 cgggttcggc atcttgatgt gggttgcctc cggggtactt ctctaccaga ggcgcaagca
     3121 gctacgcaag acggatctca tgcgcagggt cgaaacctct caggcttcgc aggtatcggc
     3181 tctatccgcc ggcacgcctg tggaggtgaa gggatttgtg cgctgcgagg aaccactcaa
     3241 gagcgagatg gccgggcacg agtgcgccta ctatctgtct caggttatcc gcgagtacca
     3301 cgagaccgac agggacgcgg atggagacat gcagacgagg aggcgatcgg aggtgatggc
     3361 ctcaaacgag cggttcgccc ccttcgtcgt tgaggatgat tcgggagtgg tcggtatccg
     3421 cggtgagggc gccgaggtcg atgctctcga ggtgatgaac cgattcgaga acgacacggg
     3481 aggcgaaggg agcatcacag tgggcggcat ctcggtaaat ctgggcggag gggcgcgcac
     3541 catcggctac cgatacgtgg agaccgtcct gcctgtcgat gcgccggtgt acgtgctcgg
     3601 cgtggcccag gaggacgggc acatcggttc ccccgtggag gaaggcgggg aggagcggtt
     3661 cctgatcagc caccgctccg aggaacaact ggagaagaag tacaggaggt ccgccctcct
     3721 gctcggcttg cttgccgtcg gactgttcct gttcggggcg atcttcgtcg tagccggcgt
     3781 ggtagccggt gtcttttcca tgggtgcctc tgccgccgtc gagccgtcgg tagctgtcgc
     3841 ggagcgaatc ctctcagggt gaacggcaga ccaggcagga tgcgaagccg tctcacagta
     3901 gctacgctct tcgcaggatg ttgcgcgctc gttctgacag cgtgcacctc tggtaccatc
     3961 acaggctccg agcaggcctg caagtccaca ggtggtctct tcgccgataa gcaggtcgcc
     4021 tgcaccggaa gcgtttccgg cgtgcggggc gaaccctcgc tcggcatcat cgacaccgac
     4081 gaagacctgt ccggagacta caagctcgat gcgaccttca cggtggagaa ggggacggcg
     4141 tacgtcagca cggccgaagg cgggcaggct gggggcaaag tcactccgga cgagccgctg
     4201 cgcatcaaca ccgtcgtcgc actcgacgaa agcgatgaag atgtctcagt caacctgaaa
     4261 gtccctggca aagaggtcaa ggacctgcgt tacgaggcca cgctgcttcc ccaggattga
     4321 cgactctccc tacttgatac tcttcaaaaa gtcattcagg gcagcgttga attcccctgg
     4381 ggcttcgagg ttcgacaggt ggcctgcatt ggggatggtc acgtggtggg cgcctggtat
     4441 cttcgcggcc atcgcgctca tgacctcggg ggaggagatc ccatcctcct cgccgccgac
     4501 gacgagggtc gggacaccta tctcgccgag gagcggagta gagtcaggcc gatcgcgcat
     4561 agcgccaagg gcggcgaccg cgccgtcggg ggagttctcc aggatcatgg tccgcacctt
     4621 ctccaccaac gcctcgtcgt tctgcagcgt gtagggggcg aggaggcgct ccaactggag
     4681 cttcacgagg accccgaccc cttcctgggc gacgcggagg gccatctcgt tgcgggtctc
     4741 gcgcatctct tcggagtcgg gttcagggcg ggtgttggcg aggacgagcg cggagatcct
     4801 ctcggggaag accctcatgc actcgaaagc gacgtagccg cccatagaga cgccgccgag
     4861 gacgacccgt tcgagatgca gcctgtcgag gagcctgcgc accccttccg catagtagtg
     4921 gacgtcgggc tgggccggcg tacgggggga gcgtccgaat ccggggtaat ccggggcgac
     4981 gacgcgacgt tcccccgaga aggccgtcat ctggggctcg aacatcctgg cgttcaacgg
     5041 gaaggcgtgg agcaggacca ggggaacccc gtctccatct ccgaggtccc tgtacgacat
     5101 cgccacgccg gaaggtctgt gatccactac gctctccttt tgattagcat ttcagcctgt
     5161 cagcttcctg gtcttgcggt gaatgttccc aacgagaaac ccccgccgcc acagatggta
     5221 gcgttttcga cggatggttt ccgcgtacac tttttccctg atgcctgaaa cctgatgcct
     5281 gaagcctact gcaaagcgtt tgcgagccgg cgcacgccgt cgcgtatccc cgcttcgtcc
     5341 accgctgcgt atccgagcat gagacctccc tgcccgtcag gacgggtctt gtagagcgag
     5401 acagggggcg cttccacacc ttgcgtggcg gcgagcctcg acgcctccct atcgtcgatg
     5461 ccctcggaga gccaccccac gagatgcatc cctgagctgg cggggctgga gtcgagaagc
     5521 ccgcggagct cgtgtgagat ctcctccacc agcacagcct gcctggcggc gtagagagcg
     5581 cgcatccggc gcaggtgcct gacgaagtgt ccccctgcga tgaactcggc gagtatggtc
     5641 tgctcgacaa tgggcggatg ccggtcggtg agctcgcggg ccgcgacgaa ggcgtctacg
     5701 aggtcgggtg gaacgaccag gtagcccagc ctgagggcgg ggaagaggac tttgctgaaa
     5761 gtgccgacgt agacgacgca cccctctgta tccagtcctt gcaaggcttc gagcggtcgc
     5821 cccgtataac ggtactcgga gtcgtagtcg tcttcgatca cccacgcccc ggaactactc
     5881 gcccaccgga gcaactccag ccgccgggcg aggctcatgg ttacgccgag cggatactga
     5941 tgcgaaggcg tgatgcagac cagacgagcg ccgggctcgc gctcgatccc cgtcgtcact
     6001 atcagccctt cctcgtctac cggaaccggc acgagccggg cgcctgaacc tgagagagcg
     6061 cccctggcgc ccacgtaccc tgggtcctcg acccatacgg catcgcctgg gtcgaggagg
     6121 acgcgcgatg cgagatccag ggcctgctgc gagccggaga ccactatcac ctgctcaggc
     6181 tcgcaccgca cggcccgcgc cgcccgaaga tgagccgaga tctcctcccg cagcggccag
     6241 tatcccgcgg ggtccccgta accgagcatc gctcccgacg gtctccgcca cacgctaccc
     6301 gagatcctgc gccacactcc gtaagggaac tgatcgagcg caggcactcc aggtcggaaa
     6361 gctcgtggtg ttcccatgtc ccatgcaatc gtcgtgggcg tcgcagccag caaagtcccg
     6421 cgcctggaga gtccccgccc cgatcgtaga ggaccctgtt cgtggatcct ccggccctgc
     6481 acaccaagca tctcttcagg tagtgaacgc gcgacgtagg tgcctgaccc gacctttcct
     6541 tccaggtatc cttcggcgag caggtgctcg aacgccgtca ccacggtcga gcgggatacg
     6601 cccagctcgg tggcaagcgt ccgcgtcgaa ggcagccgtg tgctcgcagg gagccgacca
     6661 cccagtatct cagtacgcat cctctcgtag agttggcggt agatcggcgc gtccgatgaa
     6721 aagtccaggg agatccagac cggcaccccg gccgccttct tagccatgct caacctccag
     6781 acgttccacg tctaaatggt ctggtcagat tacatgatag tggatctggt acagagtcca
     6841 cattccgtta ccatgttgtt cgaaggggag cggtccggag gtgctctggc gaaggggtgg
     6901 tctgtatgtg gaagaacttg acgagtgtgc tggaaggggg gatcgtgcgc ctcgagccgc
     6961 tggcgcgcgg tcacgagcaa gggctcttcg aggtcgcgcg ggacgagagg atctggcgct
     7021 ggatgcccta cgacgccggt aagtccagcg agacgttcaa cacctggctg gaggacgccg
     7081 tggccgcttc cgaggctgga agcgagggcg cgttcgttac cctcgacgca cgcacggggg
     7141 agcccatcgg cagcactcgt tatctcgcac ttcgtgccga gcacaggggg ctggagatcg
     7201 ggtggacctg gctcgcgcca tcgcgctggc agtccggcgc caacgtcgag gcgaagctgc
     7261 ttatgctcga gcacgccttc gagcgcctgg gatgcctgcg ggtcgagttc aagaccgacg
     7321 cccgcaacga acgttcccgc gccgccctgg ccgcccttcc cgcgcagttc gagggcgtct
     7381 tccgcaagca catgctcgtg cgtgacggcg agcgccgcga ctccgcctac tacagcatca
     7441 tcgacgacga gtggcacgag gtcagggata acctgaggcg gcgactggcc gttgtttgag
     7501 agatctcgtc cagcgaaagg aggatgtcgt ggcacgcata gaaggcgtag atccggagcg
     7561 ggtggagaag cgcatccggg ccgttctcga ggcgcaggcc gacagatggg gcgcaccgct
     7621 tctcaaccat ctgatctacg cccgccgtcc gagtatctac aggggtgtac ggggcatgtg
     7681 ggcaggacta gctgtttcgg ggctactcga ctccgggctc gttgcgcttg tgaatcgcag
     7741 ggtcgcggcc ctcaacggct gcgatttttg acaggatatc aacgctgccg tgggtagcga
     7801 gctggggatt tctgacgaga agatccttgc cctcgaagag tacgcgacga gcccgctcta
     7861 cgacgaccag gaacgtgtgg cgctggagta cgccgatgcc atgaccctct ctgatagtga
     7921 cgtcgaagaa gagctgttcg agcgcgtgcg ggccgtcttc gacgaggatg ccgtggtcga
     7981 gcttacggct gtgatctcct gggagaactc ttcgagcaag ttcaacaggg ccctgcgcgt
     8041 accttctcag ggactctgga agcgggagca tggagcagga ggaagtgagt gagcggtgag
     8101 gtgaggaggg gtgcagggac gcgcagggcg acggtcgctg aggcgatggg gcggttgcct
     8161 gggtcaggcg gagagcgatt cgcgaaggtg ctcgagcacg ggagcatgga ggttgagatc
     8221 tacgcgcccc gcgactcgga tccacagacg ccgcacaccc gcgacgagct gtacgtggtc
     8281 gtgggcggaa gcggggagtt cgtaaacggg cgggagcgca attctttcgg tcctggcgat
     8341 gtgttgttcg tgcctgctgg cgtcgagcat cgcttcgagg atttcacgga cgacctcgtc
     8401 acgtgggtca tcttctacgg tcccgaagga ggggaggctc gatgaccttg cgcctgcacg
     8461 acaacctgtc ttcgggaaac gggtacaaag ttcgtctact tcttacgcag ctcggcatcc
     8521 cgttcgagcg gatcgagtac gacataacaa cgggtggtac tcgcacccct gagttccttg
     8581 agaaggtcaa cgcgaacgga cgtatccccg tgctcgagac cgaggaaggc gagtttctgc
     8641 cggaatccaa cgcgatactt ttctacctcg cggacggtac ccccctccta cctgacgacc
     8701 ggctcgggag ggcgatggcg ctgcaatgga tgttcttcga gcagtacagt cacgagccga
     8761 acatcgccgt cgcccgcgcc tggcttcacg tcttcgacgt aaggatgacg gaggaacgtc
     8821 ttggcgtcct ggaaaccaaa cgaaagctcg gttacgacgc gctcggggtc atggagaagc
     8881 acctgaaggt caacgacttc ttcgtcggag actactccat agcggacatc gccctatacg
     8941 cctacaccca cgtcgccggc gaaggaggct ttgatctcga cggctttccc gccgtcctcg
     9001 cgtggttgga acgagtcgct tcgcagcctg gacacatacc gataacgcag ggctgaaaga
     9061 tcagccagag aggagccttc atgcagaccg aaccccgcaa ctcacatcct tttcacatgc
     9121 acgatgcgat cctggcccag ccggaagcat tcgtgcgggc cgtcgagcga aacgaagatg
     9181 cgctggacga gttcgcttct cgaatagctt catgcgagag gatcttcctg tcgggtatcg
     9241 gcacctcgca ccacgcgtcc ctggtcggcg agtgcctgat gcgggcctac ggcggcggtc
     9301 ccgacgtccg cgccttccac tccttcgacc tcgcgctcta cgggccacag atgcggcctg
     9361 aggactgcgt cgtcgccgtg agccaccgcg gcgccaaacg ctacacggcc ctcgcgctgg
     9421 accgtgctag agagatgggt tgcatgacgg ctctggtaac aggcgaagca ggatcgggcg
     9481 agggtcgggc cgacgccctg ttccgaacgg tggcgcagga gaggtcgccc gcccacaccg
     9541 tcagctatac gggcgccgtg gccgtgctcg cgctcctcgc gggacggctc ggctaccatc
     9601 ggaccggctc agcgacgctg cctgacgagc ttctgcggga tgaggttcct ggggcgttgc
     9661 gtgcggccct gggcacggaa gaagaagtgg cggcgctcgc gcgagagcat gttgggcggc
     9721 gcaggatctg gctcgtaggt ggcgggccag ccgcggtaac ggccatggag acagcgctca
     9781 agatcaaaga gacctcctac ctccaggccg agggcatgtc aacggagacc atgctccacg
     9841 gccccttcca gtgcgtcgag gaggacgacc tgttcgtcct catagccccg tcgggcgcgg
     9901 ccagggagcg tacgctcgag gtggctgagc ttgccgaaat ggtcggggct acctgcctga
     9961 tcctcggcga cgacacgacc gaagctcctc tggacgatgt gagtattctg agcgtgcccg
    10021 aggtccccga gccgtttagc gccctgagtt gcatagttcc tttgcaactc ttcgcttacc
    10081 atctcgcgct cgcgcgcggg accaatccgg acagcttcag gggcgacgac ccgcgcttcg
    10141 cgagggccga cgtttctggg cggttgtagg tagacttggc gcccgtgcgt ctgacctaca
    10201 ctcttctatt agcatacaat c
//
LOCUS       DATAIR010000624         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_204197, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000624 DATAIR010000000
VERSION     DATAIR010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204197"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1284)
                     /gene="dxr"
                     /locus_tag="VHM69_14800"
     CDS             complement(154..1284)
                     /gene="dxr"
                     /locus_tag="VHM69_14800"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2741711.1"
                     /translation="MRRRLTILGSTGSIGQQALDVVRRYPDRFEVVGLSAGTDADALA
                     IQAAEFEPEYLALESTDASPLEGLATEVIVGPGSAERLAGVPSDVVLNGIVGFAGLAA
                     TVGALQAGNRVALANKESLVAGGEWIMELASGNHIIPVDSEHSAIFQCLEGRREEEIR
                     GVLLTASGGPFFSFEKARLVEAGPEDALKHPTWRMGRKITIDSATMMNKGLEVIEAHH
                     LFGVSYDDVCVVVHRQSVVHGGAFFEDGSVVLHAALPDMRLPIAYGILYPERVDVGAV
                     PVPFDGVSWSFEEPRDDVFRCLPLAIEAGRAGSAYPVALNAANEVAVEAFLDGRITFL
                     RIAEVIEEVLEAVPEFGKMQSMETIKAVDGWARDEAQRRAVR"
     gene            complement(1304..>2156)
                     /locus_tag="VHM69_14805"
     CDS             complement(1304..>2156)
                     /locus_tag="VHM69_14805"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2741712.1"
                     /translation="ISPRRNSCTPSSPSRPGPDAGAASRDTVLRWRAATALVLAPIAL
                     ASIAIGKWAVLAVLLIVIAGAAYELSRALEPLPFVAALGAGALPLLLSIPYHKTGILA
                     GAVASLPWALFWLAGKPETRTLRAVLAVLLMSLWVGVPLAHLGLFPRSRYGVVLILIA
                     VVGPWISDSGAYFAGRFFGRNLLFPSLSPNKTVEGGVGGLLLTVLIIAPLSYQLLDFT
                     LTKALIMGAGVSISSQCGDLFESSLKRILDVKDLGNFLPGHGGVLDRVDSLLFTAPAV
                     YYISLLV"
BASE COUNT          421 a          670 c          703 g          362 t
ORIGIN      
        1 agatggtctt cccgaacttc tttttgaaga gcggtggacc gaaacctatg ccgaactcgg
       61 ggaccctgac gccgagggcc ttggccgtga gcatgtgccc gagctcgtgg atgactatga
      121 ggaagatgag accgagcagc gcgacgatga cggtcatcgc acggcccgcc tctgggcctc
      181 atcgcgcgcc catccgtcta cggcctttat ggtttccatg ctctgcatct tcccaaactc
      241 aggcaccgcc tcgagaacct cctcgatcac ttcggctatc ctaaggaatg ttatcctgcc
      301 gtcaaggaag gcctcgacgg ccacctcgtt ggccgcattg agcgcaacgg ggtaggcact
      361 ccccgcgcga ccggcctcga tcgcgagcgg caggcacctg aacacgtcgt cgcgcggctc
      421 ctcgaagctc cagctgacgc cgtcgaaagg aaccggcacg gcgccaacgt caacccgctc
      481 gggatagagg atgccgtagg ctatgggcag cctcatgtca ggcagcgcgg catgcaaaac
      541 gaccgagccg tcctcgaaga acgcgccccc gtgtactacc gactgccgat gcacgaccac
      601 gcatacgtca tcatatgaaa cgccgaacag gtggtgagct tcgatcacct cgagtccctt
      661 gttcatcatc gtcgccgagt ctatggtgat cttgcgcccc atccgccacg tcgggtgctt
      721 gagcgcatcc tcaggcccag cctccacgag ccgcgccttc tcgaacgaga agaacggtcc
      781 tcccgacgca gtcagcaaaa caccccgtat ctcttcctcc cgcctgcctt cgagacactg
      841 gaagatcgcc gaatgctcgg aatctacggg tattatgtgg ttacctgacg cgagttccat
      901 aatccactcg ccgcctgcga cgagggattc tttgtttgcg agggcgacgc ggttgcccgc
      961 ctgcagtgcg cctactgtgg ctgctaggcc cgcgaagccg actataccgt tgaggaccac
     1021 gtcggaaggg accccggcca accgctccgc cgagcctggt cccactatga cttcggttgc
     1081 caggccttcg agggggcttg cgtcggttga ttccagggcg aggtactcgg gctcgaactc
     1141 ggccgcctgg atagcgagcg catctgcgtc ggtccctgcg gagaggccga cgacctcgaa
     1201 gcggtcgggg tatctacgga cgacgtcgag ggcctgctga ccgatactgc cggtcgagcc
     1261 caggatcgtg aggcggcgtc tcattgtggt ttgggatcca atatcatact agtaaggaga
     1321 tgtagtatac cgcgggggcc gtaaagagca ggctgtccac cctgtcgagg acgccgccgt
     1381 ggccggggag gaagttcccg aggtccttca cgtcgaggat acgtttcagt gaggactcaa
     1441 agaggtctcc gcactggctc gagatggaga cgcctgcgcc catgatcagg gctttggtga
     1501 gggtaaagtc cagaagctga tacgacagcg gggcgatgat gagcacggtg aggaggaggc
     1561 cgcccacgcc accctcgacg gttttgttcg ggctgaggct tgggaacagc aggttccggc
     1621 cgaagaagcg cccggcgaag tacgcgccgg agtcggagat ccagggccca acgacggcga
     1681 tgaggatgag gacgacgccg tagcgggagc gagggaagag cccgagatgc gccagcggga
     1741 cgccgaccca gagcgacatg agcaggacgg cgagtaccgc gcgcagggtc cttgtctcgg
     1801 gcttaccggc gagccagaac agggcccacg gcagactggc gaccgctccg gccaggatac
     1861 cagtcttgtg ataggggatg gagagcagga gcggaagagc gcctgcgccc agcgccgcca
     1921 cgaaggggag cggttccagg gcgcgcgaga gttcgtaggc ggccccggcg atcacgatga
     1981 gcaggacggc gaggacggcc catttcccga tcgctatgct tgcgagcgcg atcggcgcca
     2041 gaaccagggc tgtggctgcc ctccaccgta gcacggtatc cctagacgcc gccccggcgt
     2101 ctggaccggg acgcgaagga ctcgatggcg tgcaggaatt cctgcgtgga gaaatc
//
LOCUS       DATAIR010000625         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_65648, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000625 DATAIR010000000
VERSION     DATAIR010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65648"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(72..239)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            449..739
                     /locus_tag="VHM69_14810"
     CDS             449..739
                     /locus_tag="VHM69_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741713.1"
                     /translation="MGIRDFIQHLKEHQDRLKRRPDLSDSEREETLKEAETMVGMADA
                     AIRELESVLKNRRVNLPEEHALRFAQDVFVYLREEEEQVKEALREPASSDNR"
     gene            complement(736..1053)
                     /locus_tag="VHM69_14815"
     CDS             complement(736..1053)
                     /locus_tag="VHM69_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741714.1"
                     /translation="MPVDLARAGEARHFIAWRGAVAGLGGGKLNDLAVAAEAVLTDLL
                     LSAREGVIQIESVHDEEVFRIFIKHPEIGERRMRDLEAVLEKFLDGHELTPTRAVLVK
                     RLR"
     gene            1156..>2089
                     /locus_tag="VHM69_14820"
     CDS             1156..>2089
                     /locus_tag="VHM69_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2741715.1"
                     /translation="MDSRSRVCSALLVAMLVAVMLAGCSEKQPVSRENHAAQASPQAS
                     SGIPADEPVAQVASQVGPSVVQVNVRAVQQTPLGVQRGEGIGSGVIYRPGGYIVTNNH
                     VVEGATGVDVAFADGTTEDAKVVGADPNTEIAVLKVDRDGLPAATFSRGDDPIVGQLA
                     VAIGSPSGFESTVTSGVVSGVGREFPSELMMGGTEAERNALTDLIQTDAAISPGNSGG
                     ALADREGEILGINVAYLPPTETGAVNLGFAIPSDTAVSVADQLIETGEVTTPYIGILT
                     TDLSPEDAGRFDLPVDSGALVEQVVPGSAADEAGV"
BASE COUNT          393 a          648 c          658 g          390 t
ORIGIN      
        1 actgtggctg accacccctg cctcccgatc cggcgccggt tctcccaaca gcaaaccact
       61 tggctcggag tgccctcttt ccttatcgcc tacgaggtta gctgtcgggt tcgggccgaa
      121 agagttaagc cctacgctag ctcgagagct ggcgattcac cccgcggacc ttcctgatga
      181 agtatccgtg tgggtccccc gcctcccggc gccacccggg agttaggcgt cctggctatg
      241 cagttgtcat ctatcggtat acgcctcgga gggcttgttg tcaacactga ttttggtcca
      301 atgcctggca gtgtttcctc cacaattctg tcgcaggatt ctgcctgaat aagcctcgaa
      361 tcgctgcagt tcgaacgcgg tgcgctggga gatggtcggg tgcttctgag attcgtagcg
      421 cggtccggag cagctataat cctcctccgt gggcattagg gatttcatac agcacctcaa
      481 ggaacaccag gaccggctga agaggagacc agatctctcg gactcggagc gcgaggagac
      541 tttgaaagag gctgagacga tggtcgggat ggcagacgcg gccatcaggg agctcgagtc
      601 ggtcctcaag aatcgtcgag tcaacctccc cgaggagcac gccttgcgct tcgcccagga
      661 cgtcttcgtc tacctcaggg aggaagaaga gcaggtcaag gaagccctcc gcgaaccggc
      721 ctccagcgac aaccgctaac gcagccgctt tacgaggacg gcacgggtcg gggtgagctc
      781 gtgcccgtcg aggaactttt cgagcacggc ttcgaggtct ctcatccggc gctcccctat
      841 ctcgggatgt ttgatgaaga tcctgaagac ttcttcgtcg tgaacggact ctatttggat
      901 gactccctcc cgcgcggaga gtagaagatc cgtgagcacg gcttcagcgg cgacggccag
      961 gtcgttcaac tttccaccgc cgaggccggc gacggcgccc ctccatgcga taaagtggcg
     1021 ggcctcgcca gcgcgggcga ggtctacggg cacctctctc ctgttggtcg cggcatccac
     1081 gtacaaaggg tacagtgtag tgcccttcgt atagactctg tagcgaacga cgcgcccaca
     1141 ggaggttctg gtacgttgga ttcgagatcc agagtgtgct cggccttgct cgtcgccatg
     1201 ctcgtcgcag taatgctggc aggatgttcg gagaaacagc ccgtatccag ggagaaccat
     1261 gctgcccagg cctctccgca ggcgtcctcg ggaataccgg ccgacgagcc tgtggcacag
     1321 gtcgcctcgc aggttggacc gagcgtcgtt caggtcaacg tcagggctgt gcagcagacg
     1381 ccgctagggg tccagagggg cgaggggatc gggagcggtg tgatctaccg ccccggcggc
     1441 tatatcgtca ccaataacca cgtggtcgaa ggggctaccg gcgtggacgt cgccttcgcc
     1501 gacggcacga cggaagacgc aaaagtagtc ggcgccgacc cgaacaccga gatcgcggtt
     1561 ctcaaagtgg accgtgacgg cctgccggca gccaccttca gccgtggaga cgaccctatc
     1621 gtcggccagc tcgcggtcgc catcggcagc ccttcgggct tcgaatctac ggtgacctcc
     1681 ggcgtcgtca gcggcgttgg ccgcgagttt ccttccgagc tgatgatggg cggaacggag
     1741 gctgagagga acgcattgac cgacctgata cagaccgacg cggccatctc ccccggaaac
     1801 tctggcggcg ccctcgcaga ccgcgaaggt gagatcctcg gcatcaacgt cgcatatctc
     1861 cccccgaccg agacaggggc ggtgaacctc ggcttcgcta taccgtctga cacggcggtc
     1921 tctgtggcag accagctgat cgagacaggc gaggtcacca ccccctacat cggcatcctg
     1981 acgacggact tgagccccga ggacgcggga cgattcgacc ttcctgtaga ctctggcgcg
     2041 ctggtggagc aggtcgtgcc aggcagtgca gccgacgagg ccggggtgc
//
LOCUS       DATAIR010000626         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_458215, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000626 DATAIR010000000
VERSION     DATAIR010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458215"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..996
                     /locus_tag="VHM69_14825"
     CDS             205..996
                     /locus_tag="VHM69_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741716.1"
                     /translation="MEGLSATLVYVGIGSVLATGVGILIVAMLFLRTARRYVDLAEER
                     LELLRESEALLLKVARQQSHAMEEESREPEPERRERVPEVIGHTLSRRAGEASPGSRR
                     PEAPGIRGQVPSAGDEEQGREKTPKASDPGGATARPKDGKPLLGVRVAHPDDDVTRRD
                     WNSSATKFFQKCYDRYLEHYEGYVRLAGRIHQMQDETSPGTLGRREWEEKLRRAYDAI
                     ETTTHRLDILEEHYPELATDSDRISSRISTARLHAGLAERFGYPG"
     gene            complement(1091..1873)
                     /locus_tag="VHM69_14830"
     CDS             complement(1091..1873)
                     /locus_tag="VHM69_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741717.1"
                     /translation="MGRRALLLLTTIGVGVLLSSGVALAANLISCTGGLCEGTTGDDE
                     MIGTSATDHMYAYQGNDTLRGGRSFDDLRGGQGNDDLNGGRGNDQYNIYDNNWGADTI
                     SADRSGVKDWLIFQTSGALMIDLKPSSTSDEVTSGTNTINIAPEVVIEWVQAGSESDT
                     IKGNSADNYLSGAGKDDTLIGRKGDDDLLGDIVVFGNNGKDTLIGGPGNDDLDGGPGN
                     DTFFAQDGEADEITCGPGTDVVVDADPSLDSRSLDCIDFVHP"
     gene            complement(2667..2993)
                     /locus_tag="VHM69_14835"
     CDS             complement(2667..2993)
                     /locus_tag="VHM69_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741718.1"
                     /translation="MHPDQTVSEMAQEVIRRQARTLADQSGQPFEWAMSDTLKSDAAR
                     QLRQLAEGEYGEWKAAQWQARLPWARAEERHYSWLESYMGWLKGKESRQEYHALLEEE
                     LASLRG"
     gene            complement(3350..3422)
                     /locus_tag="VHM69_14840"
     tRNA            complement(3350..3422)
                     /locus_tag="VHM69_14840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3388..3390),aa:Ser,seq:tga)
     gene            complement(3562..3634)
                     /locus_tag="VHM69_14845"
     tRNA            complement(3562..3634)
                     /locus_tag="VHM69_14845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3599..3601),aa:Arg,seq:cct)
BASE COUNT          755 a         1119 c         1146 g          808 t
ORIGIN      
        1 cgtctttcga cggagttgtc cttcaaagta ggccatggct gggatccttt ccgagtgtca
       61 ggtgtgaaca tagtacctgt atttgcgggt aaggacttgg ccggtgtcct ttgcggttca
      121 tcctgttgct atcctgtcgc tagtgcactt taatacaggc aaagcaggtg cctcggactc
      181 ggagatggcg gtgttcggag ggagatggaa gggttgagcg cgacgttggt gtatgttggc
      241 atcggttccg tcctggctac gggcgtgggg attctcatcg tcgccatgct tttcctgcgc
      301 acggcgcgtc gatatgtgga tctggccgag gaacgcctgg agctcctgcg cgaaagcgaa
      361 gcgcttctgc tgaaggttgc gcgacaacag agccacgcta tggaggagga gagtcgggag
      421 ccagaaccgg agcgacgaga gagggtccct gaggtaattg ggcatactct ctcccggcgg
      481 gcgggcgaag cgtccccggg cagcaggcgg cctgaagcgc cgggtatacg cgggcaggtt
      541 ccctcagcag gagatgaaga gcaggggcgg gagaagacac cgaaggctag cgaccccggc
      601 ggcgcgacgg cgcggcccaa agacggtaag ccgctccttg gagtcagagt ggcgcatcct
      661 gacgacgatg tgacgcgacg tgactggaac agctccgcga cgaagttctt ccagaagtgc
      721 tacgacagat acctcgaaca ctacgagggc tacgtgcggc tcgccgggcg gatacaccag
      781 atgcaggacg agacgagccc aggtaccctc ggaaggcggg agtgggagga aaagctgcgg
      841 cgcgcctacg acgccatcga gacgaccacc cataggctgg acatactcga ggagcactac
      901 ccggagcttg cgacagacag cgatcgtatt tcgtcccgca tcagcacagc ccgcctccac
      961 gccgggctcg cggagcgctt cggttaccca ggctgagcgg tttacgagcg cagcccctta
     1021 aggtataggc agcagggccg gtgcgcatag aaggatgcgc gggcgctgag tttgtttcac
     1081 gtccctctat ttagggatga acgaagtcga tgcagtccaa cgatcggcta tcgagtgagg
     1141 ggtcggcatc gactaccaca tcggtgcctg gaccgcaggt gatctcgtcc gcttcaccgt
     1201 cttgcgcgaa gaatgtgtcg ttgccggggc caccatcgag gtcgtcgttg cccgggcccc
     1261 cgatcaaagt gtccttaccg ttattaccga acacgacgat atcgccgagc aggtcgtcat
     1321 cgcccttgcg accgatcagg gtgtcgtctt tacctgcgcc cgagaggtag ttgtcggcgc
     1381 tgttgccctt gatggtgtcg ctttcggacc cggcctggac ccactctata acgacctcgg
     1441 gggcgatgtt gatcgtgttc gtgcccgaag tgacctcatc gctggtcgag ctcggcttca
     1501 gatctatcat cagggcgccc gaagtctgga agatcagcca gtccttcacc ccggaacggt
     1561 cggcggagat ggtgtccgct ccccagttgt tgtcgtagat gttgtactgg tcgttgcccc
     1621 gtcccccgtt aaggtcgtcg ttgccctgtc caccacgcag atcatcgaag ctcctgccgc
     1681 cgcgtagggt gtcgttgccc tggtaggcat acatgtggtc ggtggcgctc gtgccgatca
     1741 tctcgtcgtc accggtggtg ccttcgcata accctcccgt acaggatatt agattcgctg
     1801 ccagcgccac cccgctggag agcaacaccc ctacccctat cgttgtaagc aagagtaaag
     1861 ctcgccttcc catgctcttc tccctcgtgc ccaagtggtt actcaagtac actaacagtg
     1921 gagaaggggc cggcgaatcc cccaaaatga ctaacttttg accttcctcg tgaaggatgc
     1981 accggggcca ccatgtagga ctccggcgca caaggtcaat gctcttcaca cgggcttttc
     2041 tcagcggttc ccagttcgcg tgccggcgag agagatctcg tctgacctga gttcgctacg
     2101 taacaagaag cccgagaggt actctttacc tggccgcgga tactcttgcg ctcctgtcga
     2161 tagtcacagg gcacactcgg gtgaataacg gaccggggca tcgttgagga aagaatccgt
     2221 aaaggcatcg tcggaacccc ggtccgtgag tcaatcgcca ggcctcgggt ccgagggaga
     2281 agggacctgt acccgacatg ccagacaccg tacgatacgc gggaactcgc cgcatctgcc
     2341 gtatggcata tctttggtat cccacgagaa aagctcaggc ataggtcgag gagcgcggcg
     2401 gccttgggac cgggcctcaa cgaaaagtgc atgagagaac gagaggcccg gccagtgagg
     2461 agggcggact caaaggggag cgaatccgtt cccggcctca agactactgt aggagctgat
     2521 ctgggccggc gcatctgccg actggcatat ctttctctat gatgttcacg ggttgggact
     2581 accccgtaga gatattccgg cccacacaag ccccaggtag cgtcggaccc agatcaaagt
     2641 gttgacgaaa atgctggtca ggcggctcag cctctaaggc tagcgagctc ttcctccagc
     2701 agcgcatgat actcttgccg gctctcttta cccttgagcc agcccatgta gctctccagc
     2761 cacgagtagt ggcgctcctc agcacgcgcc cacggcagtc tggcctgcca ttgcgctgct
     2821 ttccactccc cgtactcgcc ttcggctaac tgcctgagct gcctggcggc gtcggacttc
     2881 aaggtatccg acatcgccca ctcaaagggt tgtccactct gatcggccag ggtcctggcc
     2941 tggcgcctga taacctcctg ggccatctcc gagacggttt gatctgggtg cacgatttct
     3001 tatcccttcc tctaactgtt ccccgatgga ctgactagag cctgctctga ctgatgctgg
     3061 catgacgccg aggttcgcgt atcactcaaa tgcagtattt ttcgttaact ccgagttaaa
     3121 tgcttgatgg attcccggca catcgttcca cttcgtcatc ttcggtgtca catgtgtggg
     3181 cccgcgttcc gagcggagat cccagctgac gtgaaagcta ccctcgcgga ggccgtgggc
     3241 ccacagcaat caaggtgcct tggtggatat gattgtgcat ttgccagata gcgtatttca
     3301 tatcgcgtgg acccagtccc agttcccgct ccctctcgcg ccgcaaggtt ggttccagga
     3361 tagggactcg aaccccgatc ttcgctttca aggtgcgagg tgctgccgtt acaccatctt
     3421 gactctctga tcctatactt ctccagaagt cttcctgaaa agcctcttca gcggcaaccg
     3481 tgctggtgaa atgactggtg ggttagttgg atgcggggtg gcatattcag ggaatctagc
     3541 ttatctaagc caatttgaga gtgcgcctgg aaggattcga acctccgact cgcggtttag
     3601 gaaaccgctg ctctatcccc tgagctacag gcgcaaagcg ccaacagttt aacgcgcaac
     3661 tgccatgctt accaccgcgc tgcatccagc cagcttgccc gcgctatcga aggggtttgc
     3721 agcagtttcg gcccccgcgt atcgcgcgga caggctgtag tagatcggct gagagcgaaa
     3781 aacctgaagc tgaagtgctg accggctgaa atgctgaagt gctgaacg
//
LOCUS       DATAIR010000627         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_158498, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000627 DATAIR010000000
VERSION     DATAIR010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158498"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHM69_14850"
     CDS             <1..182
                     /locus_tag="VHM69_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741719.1"
                     /translation="SFGVGISLDPRPGTRDHTQRAGLHDLVGRAPHLREVLLHGRPVD
                     DGKRGLRGAHHVVHR"
     gene            330..692
                     /locus_tag="VHM69_14855"
     CDS             330..692
                     /locus_tag="VHM69_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741720.1"
                     /translation="MQYMKAYSEDLRQKIVDALKRGMPNSEAASLFGVSLSSVKRFAR
                     LERQGDSLAPRKPPGRPPKSNDTIRRLLSEELADHPAATAPERRRYLERMTGETMSDS
                     TLRRLVRRLGHSRKKDHP"
     gene            734..1294
                     /locus_tag="VHM69_14860"
     CDS             734..1294
                     /locus_tag="VHM69_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2741721.1"
                     /translation="MVSGELCAKRLVFVDEMGSNTSLHELYAYAPRGESVLLGGAQNR
                     GKNTTLLSSLSSMSLRGMGPSMVVEGGTDGAVFEGYLREMLLPALEEGDVVVMDNLSV
                     HKGEGVREMIEGAGAHLLYLPPYSPDFNPIEEAFSKIKNLLRKAGARVHEALVVVLGE
                     AISEVGEDDAKAFFEHFGYHEAVQLL"
     gene            1423..2529
                     /locus_tag="VHM69_14865"
     CDS             1423..2529
                     /locus_tag="VHM69_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX2741722.1"
                     /translation="MQVFEKLAEYRYEQLVFCHDKATGLKAIIAIHDTTLGPALGGCR
                     MWPYATEDEAIVDVLRLARGMTYKAAASGLNLGGGKSVIFGDPDHDKSEALFRSFGRY
                     IETLGGRYIVAEDVGTSTEDANFIRVETSHVVGVDVTRGGSGDPSPFTALGVRQGMRA
                     CAEEVFGTASLEGRTVAVQGLGHVGYHLCRLLNEEGARLIVTDLHGDVVERAAREFGA
                     KPVEPDEILPLPCDILAPCAMGAIVNDQTLPELRCGIIAGSANNILHEARHGEVLAER
                     GILYAPDYVINAGGLINVADELEGYNERRATKRVMRIYDSVKSIIAISKRDGVPTNVA
                     ADTMALERISAISSMERLHTGHPYGQLSRRRETL"
     gene            complement(2889..>3453)
                     /locus_tag="VHM69_14870"
     CDS             complement(2889..>3453)
                     /locus_tag="VHM69_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX2741723.1"
                     /translation="FAEREGVKLTYLPFIVGAVVESLKENPVLNSVWDGDRILLRKQI
                     NIGIAVDLDDALIVPVLRDADEMNLVGLARRIDEVVNKARARRLSPDEVSGGTFTVNN
                     PGALGSVVSTPIINHPQAAILSAEAVVKRPVVVDHDGGEAIAIRRMMNLEVSFDHRIL
                     DGGAALRFLNAVKRRMEGYRAKSSIRG"
BASE COUNT          702 a         1027 c         1074 g          650 t
ORIGIN      
        1 acagcttcgg ggtcggaatc tcgctcgatc cgcgtcctgg cactcgagac catacgcagc
       61 gtgctggatt acacgacctt gtaggccgcg cgccacatct gcgcgaagtc ctgctccacg
      121 ggcgtccggt cgatgacggc aagcgcggtc tacgaggagc gcatcacgtc gtacatcgct
      181 gaccgctcga tattgagcat caaccgggtt cacatcggtc ccacaaacgt tccgttaggt
      241 atagttgaga agtttagaga cctattatcg agacaaacct ttctgcgtcc cttacagcgg
      301 tgagcaaaaa ccttgacccg catgagatca tgcaatacat gaaagcctac tcggaagatc
      361 tcagacagaa gatagtcgat gccctaaagc gaggcatgcc caacagcgag gccgcctccc
      421 tcttcggggt gagcctctct tcggtaaagc gcttcgccag gttggagcgc cagggggact
      481 cgctggctcc gaggaagcct ccgggaagac ctccgaagag caacgacacc atcaggaggt
      541 tgctttccga ggaactcgcc gatcacccgg cggcgaccgc cccggaaagg cgacgctacc
      601 tggagcgcat gacgggcgag accatgagcg actcgacgct gaggcggctc gtgaggaggc
      661 ttggccatag ccgaaagaaa gatcacccct agcctccgag cgcgacgagt tcccaagagc
      721 ggcctggcgg gtgatggtct cgggggagct ctgtgccaag aggctggtgt tcgtggacga
      781 gatgggatcg aacacctccc tgcacgagct ctacgcctat gctccgaggg gagagagcgt
      841 attgctcggt ggcgcgcaaa accgcggcaa gaacaccacg ctgctttcga gcctttcgag
      901 catgagcctt agggggatgg ggccttcgat ggtggtcgag ggcgggaccg acggggcggt
      961 gttcgagggc taccttaggg agatgctcct gcccgcactt gaggaggggg acgtggtggt
     1021 tatggacaac ctctccgtcc ataagggcga gggggtcaga gagatgatcg agggcgcggg
     1081 cgcgcatctc ctctacctgc caccctactc gccggacttc aatccgatag aggaggcatt
     1141 ctcgaagatc aagaacctcc ttcgcaaggc cggggcgcgc gtccacgaag ctctcgtcgt
     1201 ggtgctcgga gaggcgatct ccgaggtcgg tgaggatgat gcgaaggcat tcttcgagca
     1261 tttcggctac catgaggcgg ttcaactatt atgaccgccg ctgtagcgct accggaatcg
     1321 tgatttggca taaacaaact tatcctgtgg cggttcgatg gacgcctggt agtagaatgt
     1381 gatttcggga cgatgttccg gttggggaaa ggagaaacga atatgcaagt cttcgagaag
     1441 ctggcagaat accgctacga gcagctcgtg ttctgccacg acaaggccac aggattgaag
     1501 gcgatcatcg cgatccacga caccaccctc ggtccagctt taggcggctg ccggatgtgg
     1561 ccttacgcca cggaagacga agccatcgtg gacgtactac gcctggcacg tgggatgacc
     1621 tacaaggccg ccgcgagcgg cctcaacctt gggggaggga agtccgtgat cttcggcgac
     1681 cccgaccacg acaagtccga ggcgctgttc cgatccttcg gccggtacat cgaaaccctg
     1741 ggcggacgat acatagtcgc cgaggacgtt ggcacctcca ccgaagacgc gaacttcatc
     1801 agggtcgaga cctcacacgt agtcggtgtg gacgtgaccc gtggcggctc cggcgatcca
     1861 tcacccttca ccgccctggg cgtgcgccag gggatgcggg cctgcgccga ggaagtcttc
     1921 ggcacggctt cgctggaggg ccggacggtg gccgttcagg ggcttgggca cgtcggctac
     1981 cacctgtgcc ggttgctgaa tgaggaggga gcacgcctca tcgtcactga tctgcacgga
     2041 gacgtggtcg agcgtgcggc gcgggagttc ggtgctaagc cggtggagcc ggatgagatc
     2101 ctgcccctgc catgcgacat actggccccg tgcgcgatgg gcgccatcgt caacgaccag
     2161 acgctgccgg agctgcgatg cggcatcatc gccggtagcg ccaacaacat cctgcacgaa
     2221 gcccgccacg gcgaggtgct cgcggagcgc ggcatcctct acgctccaga ttacgtcata
     2281 aacgctggcg gtctcatcaa cgtggccgac gagctggaag gctacaacga gcgccgggcc
     2341 accaagcgcg tcatgcgcat ctacgactcc gtaaagagca tcatcgccat ctccaaacgc
     2401 gacggcgtcc cgaccaacgt cgccgcagac accatggccc tggaacgtat atccgccata
     2461 agctcgatgg aacgcctgca taccggccac ccatacggcc agctcagccg ccgccgggag
     2521 actctgtaac tggggtcagc aagctggcaa caatggtcag cttagtgatg ccggagagat
     2581 tcgacgaaat ccactctgcc atcgttagtg aagggtatac cctcatcgag tagttgctgc
     2641 ttctgctgat cagatccatc ggggaggttt gcaagctttc catcaacacc gactacacga
     2701 ttcgtcaacc gctgatggtt gtagtttctg gcaccccgca gaagagcgcc gactggctgg
     2761 ttttggttcg gcttgtcgta tccccaaaga gatacctggg cgtaggttgt tacgcgacct
     2821 gcaggaacat tctgccgaag cacggtcacg agttgtctcc aggtcctact tctcttcggt
     2881 gccattctct aacctcgtat gctgctcttt gctctgtagc cttccatccg ccgcttgacg
     2941 gcgttgagaa agcggagggc agcgccgccg tcgaggatgc gatggtcgaa ggagacttcc
     3001 aggttcatca tgcggcggat ggcgatggct tcgcccccat catggtccac cacgaccggg
     3061 cgtttgacga cggcctcggc tgagaggatg gccgcctgcg ggtggttgat gatcggggtg
     3121 gagaccacgc tccccagcgc gccggggttg ttgacggtga aggtgccgcc ggatacttca
     3181 tccggcgaaa gacggcgggc gcgtgccttg ttcacgacct cgtctatacg gcgggcgagg
     3241 ccgaccaaat tcatctcgtc ggcgtcccta agaacgggca ctatcagggc gtcgtccagg
     3301 tcaacggcta taccgatgtt gatctgcttg cgaagcagga tcctgtcccc atcccacacg
     3361 gagttcagaa ccgggttttc tttgaggctc tctacaaccg ctcccacgat gaaaggcaga
     3421 tacgtgagct tcaccccctc acgctccgcg aat
//
LOCUS       DATAIR010000628         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_136167, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000628 DATAIR010000000
VERSION     DATAIR010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136167"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHM69_14875"
     CDS             complement(<1..240)
                     /locus_tag="VHM69_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2741724.1"
                     /translation="MRGRLIVVSGPSGAGKSTLIRASLEAVPELAYSVSATTRGPRPG
                     EVDGKDYIFLSQEEFESWIEEDRFLEWAEYSGNLYG"
     gene            complement(225..548)
                     /gene="mihF"
                     /locus_tag="VHM69_14880"
     CDS             complement(225..548)
                     /gene="mihF"
                     /locus_tag="VHM69_14880"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX2741725.1"
                     /translation="MLRRAPERTADERRNALEEANRVRFARAATKRDLKSGGLAIYDL
                     LMDPSEELRGAKVEEMLLAVKGMGRIKVTRVMREAGISRSKTLVGLTHGQRDRLIRAL
                     GCEEG"
     gene            complement(564..1490)
                     /locus_tag="VHM69_14885"
     CDS             complement(564..1490)
                     /locus_tag="VHM69_14885"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX2741726.1"
                     /translation="MVIRETGVDLTVELCGLTLGTPLIPASGTLSKEALGEVEGVYGA
                     MLPKTTTPVARAGNPPPRVAETPAGMVNSIGLQNPGVERFLQDLDAFDLGIPIIVSVA
                     GDTVAEFGGLCERLAGEDLVWAVELNLSCPNVECGGLAFCAGPASVEEVVAACRAAVP
                     GKPIFAKLTNEGVVENARAAEAAGADGLTVINTIPALTVDARARKVLVRGGLSGPAIK
                     PVALRAVYDVSGVVSIPVVGSGGVVSGTDVAEFMLAGAAAVQVGTASFVRDPQVILGE
                     FVAYLKESGLGAADMTGALRGIRPAVTGGTGR"
     gene            complement(1484..2218)
                     /locus_tag="VHM69_14890"
     CDS             complement(1484..2218)
                     /locus_tag="VHM69_14890"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX2741727.1"
                     /translation="MKFYNVQVEERRELGNYTLIRYRWNDGPVEPGQFVMARAAGYPA
                     TLDPFLARPFSFYDYDGEMASLLFEVRGRGTALLDLATNIEVTAPLGKGFAIEPDLGR
                     AALLGGGIGVAPLKVLGRRLEALDVPYDVFLGFADAGSAVVAREFAGAEVATMDGSVG
                     THGTVLDAAASLEDYGAVYSCGPNPMLAAVKLAAGEKSLCQLSVEERMGCGNGSCNGC
                     VVPVSGGGYVRSCIEGPVFSAEVLAW"
     gene            complement(2215..>4516)
                     /gene="carB"
                     /locus_tag="VHM69_14895"
     CDS             complement(2215..>4516)
                     /gene="carB"
                     /locus_tag="VHM69_14895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2741728.1"
                     /translation="DPESDEYYVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSL
                     DEIPNDITRATPASFEPALDYVVTKIPRFAFEKFPGAVPRLTTRMHSVGEVMAIGRTF
                     TESLMKAMASLEVDAQDVHAGLDEPSPYRVFAIFDALRAGMDIPEIFKRTSIDPFFIA
                     SIARIVAAEGSVSQPLRFEDLRELKRIGLTDETIAAASGDSVEVVRGVRRALGVSPTY
                     KSVDTCAGEFPARTPYYYSTYEEEDEVRRGENASVVVLGSGPNRIGQGIEFDYACVHA
                     SYALKESGYDAVMVNSNPETVSTDYDTSTRLYFEPLTAEHVLEVIRREEPDGVILQFG
                     GQSPLKLAHELEKAGARILGTSPDAIDLAEDRSRFGRLLADLGIPHPRFGTASTAEEA
                     REVARELGYPVVVRPSYVLGGRRMEIVYNDDDLDLYLRTSAGASPGHPTLIDKFMEDY
                     TEVDVDAVSDGEEVYVGGIMEHVEEAGVHSGDSSCVTPPITIHQALLQRIEDYTRQLA
                     LSVGVVGLMNVQFVVRGDDVLVIECNPRASRTVPFISKATGVPLAKVATRVLLGEKLR
                     DMRLQASTNGHFSVKAPVFPFDRFADVDPLLGPEMRSTGEAMGIDRTFGGAFAKALTA
                     AGQELPVSGRVYISVSNREKRGVVLIARAFADLGFEISASEGTAEVLKNNGLPVMIVP
                     KIGELGEDVLGLIEAGGVDLIVNTPWGRGARTDGYLIRRRALMHGVPCITTLAGAAAA
                     VQGIEARVRGGTRRVNSLQGLYAAKA"
BASE COUNT          822 a         1546 c         1335 g          813 t
ORIGIN      
        1 cccgtagagg ttaccagaat actcggccca ctccagaaat cgatcctcct cgatccagct
       61 ctcaaactcc tcctgcgaga gaaagatgta gtccttacca tctacttcgc cgggacgtgg
      121 ccctcgcgtc gtcgccgaga cggaataagc aagctcggga accgcctcta gcgaggccct
      181 tatcagtgtc gactttcctg ctccggaagg accggagacg acgatcagcc ttcctcgcac
      241 cctaaagccc tgatcaaacg gtccctctgc ccgtgcgtga gccctacgag ggtcttggag
      301 cggctgatgc ccgcctccct catgacccga gttaccttta tgcggcccat gcccttgacc
      361 gcgagcagca tctcctcgac cttggcgccc ctcaactcct cggaaggatc catgaggagg
      421 tcgtagatcg ccaaccctcc gctcttcagg tcccgcttgg tggcggccct ggcgaagcgc
      481 actctgttgg cctcctcgag cgcgttccgg cgttcgtctg ccgttcgttc tggtgcccgt
      541 cgtaacatca acaagctagt atatcatcgg cccgttccgc ccgtcaccgc aggacgaata
      601 ccccgcaaag ctcctgtcat gtccgcggcg ccgagaccgg actcttttag atacgcgacg
      661 aactccccaa gtatgacttg cgggtcacgg acgaagctcg ccgtgcccac ctgcacggca
      721 gccgcgccgg cgagcatgaa ctctgccacg tccgtgccgc tcacgacacc cccgctcccc
      781 actacgggga tactcaccac accagacacg tcgtatacgg cccgcaacgc caccggcttt
      841 atggccggtc ccgagaggcc gccccgcacg agcaccttcc gcgcacgagc gtctacggtc
      901 agcgcaggga tcgtatttat tacggtcagc ccgtcagcac ctgccgcttc ggcggcgcgg
      961 gcgttctcca cgaccccttc attggtcaac ttggcgaaga tcggcttgcc cggaacggcg
     1021 gcgcggcacg cggcgacgac ctcctcgacg gacgctggtc ctgcgcagaa ggccaacccg
     1081 ccgcactcga cgttcgggca cgagaggttc agttcgacgg cccagacgag atcttcgccc
     1141 gcaaggcgct cgcagagccc gccgaactcg gcgacggtat cgccggccac ggagacaatg
     1201 atgggaattc cgaggtcgaa agcgtccagg tcctgcaaga aacgctccac gccgggattc
     1261 tgaagcccga tcgagttgac catccccgca ggggtctcgg ccacccgggg cggcggatta
     1321 ccagccctcg caacaggagt cgtggtcttc ggcaacatcg ccccgtagac gccctctacc
     1381 tcccccagag cctccttcga gagggttcct gaagcgggga tcaggggtgt gcccagcgtg
     1441 agcccgcaga gctcgacggt gaggtccaca ccggtttctc gaattaccat gccagtacct
     1501 cagcgctgaa gacaggcccc tcgatgcacg acctgacgta accgccaccg ctcacaggga
     1561 ccacgcaccc attacacgag ccgttgccgc accccatccg ctcctccacc gagagctggc
     1621 aaaggctttt ctctccagcg gcgagcttga ccgccgccag catggggttc ggcccgcaag
     1681 agtagaccgc cccgtaatcc tccagggatg ctgcggcatc caggaccgtt ccgtgggtgc
     1741 ctactgaccc atccatagtc gcgacctccg cgcctgcgaa ctcccgcgcg acgaccgctg
     1801 aaccagcgtc cgcgaacccg aggaacacgt cgtaaggaac gtccagtgct tcgagtcgac
     1861 gcccaaggac cttgagcggc gcgacgccta tgccgccacc gagaagcgcg gcccggccca
     1921 gatccggctc gatagcgaac cccttaccca acggagcggt gacctcaatg ttggtcgcaa
     1981 ggtcaagtag cgccgtgccg cggccacgca cctcaaagag caggctcgcc atctccccgt
     2041 cgtagtcgta gaaggaaaag ggtctggcga ggaaagggtc gagggttgcc ggataccctg
     2101 ccgcccgcgc catcacgaac tggccgggtt cgacggggcc gtcgttccag cggtagcgaa
     2161 tgagcgtata gttgcccagt tccctgcgct cctcgacctg tacgttgtag aacttcacgc
     2221 cttcgccgcg tagaggccct ggagggagtt cacgcgcctg gtaccgccac gaactctggc
     2281 ttctatgccc tgcacggcgg ccgcggcacc ggcgagggtc gttatacacg ggaccccgtg
     2341 catgagggcc ctgcgacgaa tcaggtaccc gtccgtgcgc gccccgcggc cccatggggt
     2401 gttgacgatc aggtctacac ctccggcttc tatgaggccg agaacgtcct cgccgagctc
     2461 cccgatcttg ggcactatca tgacgggcag gccgttgttc ttgagcacct ccgctgtacc
     2521 ctcgctcgcc gagatctcga acccgaggtc ggcgaaggcc ctggctatga gcacgacgcc
     2581 ccgcttctcg cggttagaaa cggagatgta gacacgccct gatacgggaa gctcctgtcc
     2641 cgctgcggtc agcgccttcg cgaaggcgcc accgaaggtg cggtctatac ccatcgcctc
     2701 ccccgtcgag cgcatctctg gccctagcag aggatccaca tcagcgaacc tgtcgaacgg
     2761 gaagactggt gccttgacgg agaagtgccc gttggttgac gcctgcagcc gcatatccct
     2821 gagcttctct ccgaggagta ccctggtcgc taccttagcg agcggcacac ccgtggcctt
     2881 ggagataaac gggacggtgc gcgaggcgcg agggttgcac tcgatgacga gcacatcgtc
     2941 gcccctgacc acgaactgca cgttcatcaa cccgacgacg ccgacggaga gtgcgagctg
     3001 ccttgtgtag tcttcgattc gttgaagcag cgcctggtgg atcgtgatcg ggggcgtcac
     3061 acaggaggag tcgccggagt gaacccccgc ctcctcgacg tgctccatga tcccgccgac
     3121 gtagacctct tccccgtcgg agacagcatc cacgtctacc tcggtatagt cctccataaa
     3181 cttgtcgatc agggttggat gaccaggact ggcacccgcg ctggtcctga ggtagagatc
     3241 gaggtcatca tcgttgtaaa cgatctccat cctgcgcccg ccgaggacgt acgagggccg
     3301 cacaacgacc ggatatccca gttcgcgggc gacctcgcgg gcctcttcgg cggtcgatgc
     3361 cgtcccgaac ctgggatgcg ggatgcccag atcggccaga agccgaccga acctggagcg
     3421 gtcttcggca aggtctatgg cgtccggaga tgtgccgagg atgcgcgctc ccgccttctc
     3481 cagttcgtgc gcgagcttca gtgggctctg gccgccgaac tgcaggatca ccccgtcagg
     3541 ttcctcgcgg cggatgacct cgagcacgtg ctccgccgtg agcggctcga agtacagtcg
     3601 cgtggaagta tcgtagtccg tggatacagt ctccgggttg gagttgacca tgacagcgtc
     3661 gtagccgctc tccttgagag cgtaactcgc gtgaacgcaa gcgtagtcga actctatccc
     3721 ctgcccgatg cggttcgggc cactgccgag cacgacgacg gaagcgttct ccccgcgccg
     3781 aacctcgtct tcttcctcgt atgtcgagta gtagtatggc gtgcgggcgg ggaactcccc
     3841 tgcgcaggtg tccacggact tgtaggtcgg cgaaacgccg agggcccgcc tcacgccacg
     3901 caccacctcc acagagtctc ccgacgccgc ggcgatggtc tcgtcggtga ggccgatcct
     3961 cttgagttcc cgcaggtcct cgaagcggag cggctgcgat accgagcctt ctgcggccac
     4021 gatcctggct atggacgcga tgaagaacgg gtctatggag gtgcgtttga atatctcggg
     4081 tatgtccatc cccgcgcgca atgcgtcgaa gatggcgaag acgcggtaag gactcggctc
     4141 gtcgagtccg gcatggacgt cctgggcgtc cacttcgagc gacgccatcg ccttcatcag
     4201 gctctccgtg aacgtccttc caatcgccat gacttcgccg acagaatgca tcctggtggt
     4261 cagcctcggc accgcacctg ggaacttctc gaaagcgaag cgcggtatct tggttaccac
     4321 gtagtcgagc gcaggttcga acgaggcggg agtcgcgcgg gttatatcgt tggggatctc
     4381 atcgagcgag tacccgaccg cgagcttggc cgctatcttc gcgatcggga accccgtcgc
     4441 cttgctcgcc aacgcactcg acctgctcac cctcgggttc atctcgatca cgtagtactc
     4501 gtcactctcg ggatcc
//
LOCUS       DATAIR010000629         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_136180, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000629 DATAIR010000000
VERSION     DATAIR010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136180"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VHM69_14900"
     CDS             <1..684
                     /locus_tag="VHM69_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HEX2741729.1"
                     /translation="QTEEGLHYVGLNVPVGRMSGEQFEEAGRLAGEYGDGIRLATDQN
                     LIITGVPEDRLDDLLAEPLLARYSPNPGAFERGVVACTGSEFCRFGIVETKVRAVEWA
                     REMDERVGDLGQEAVRMHFSGCSASCAQPQIGDIGFRGETAKTRDAIVEGVDIGLGGS
                     LGKDAAFIDWVEGAKPAEEVPDALVAVFEQFEEERREGERFHEWARRKKNAELREALR
                     KPTRSGVVS"
     gene            684..>1559
                     /locus_tag="VHM69_14905"
     CDS             684..>1559
                     /locus_tag="VHM69_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HEX2741730.1"
                     /translation="MAGFDIRDMMNDIEEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGIGKDDLPELVKMCTGCSLCWDFCPRGGLQYESTWKISGNGHSIEGIGRVEE
                     SYTARVKQRIDGVQDGGFVSALLISLLEAGEIDGALLARESETERWKGEAFLATTPEE
                     VRECAGSFYNQTLALGHVDFKSSDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSSKV
                     EAITLTIALLCTKSFNYEKLMLEEIQDKRNVDLDNVGRVDIIRGKMIVQDLDGATIFE
                     EPIRDFHGAALKGCDE"
BASE COUNT          310 a          441 c          570 g          238 t
ORIGIN      
        1 cagacggagg aaggactcca ctatgtcggc ctgaacgtgc ctgtggggag gatgagcggg
       61 gagcagttcg aggaggcggg taggctcgcc ggcgaatacg gcgacggcat ccgtctcgcc
      121 acggaccaga acctcatcat tacgggcgtc cccgaagacc gattggacga ccttctcgcc
      181 gagccgctac tcgcgcggta ttccccgaac cctggggcgt tcgagcgcgg ggtcgtcgcg
      241 tgcacaggca gcgagttctg ccgcttcggt atcgtcgaga cgaaggtcag ggccgtagag
      301 tgggcccgag agatggacga gagggtgggt gacctcggcc aggaggccgt caggatgcac
      361 ttctccggtt gctcggcgtc atgcgcgcag ccccagatcg gagacatagg cttcaggggc
      421 gagaccgcga agacgcggga cgccatcgtg gagggcgtgg acatcgggct cggcggctcg
      481 ctcggcaagg acgcggcgtt tatcgactgg gtcgagggcg cgaagcccgc cgaggaggtg
      541 cccgacgcgc tggttgcggt cttcgagcag ttcgaggagg agcggcgcga gggcgagcgc
      601 ttccacgagt gggcgcggcg caagaagaac gccgagctgc gggaggcgct gcgcaagcca
      661 acgaggagcg gggtggtctc gtagtggccg gcttcgacat acgggacatg atgaacgaca
      721 tcgaggaggc tccgggcaag gtctggttct gggacctgga gaaggccgtt atcgacgcgg
      781 accgttgcgt gcagtgcggt gtgtgcgtcg ctgcctgccc gaccgactcc atcggaatag
      841 gcaaagacga cctccccgag ctcgtcaaga tgtgcacagg atgttcgctg tgctgggact
      901 tctgcccgcg cggcggacta cagtacgagt cgacgtggaa gatctccggc aacggccata
      961 gcatcgaggg cataggtcgc gtcgaggaga gctacacagc ccgagtcaaa cagcgaatag
     1021 acggcgtgca ggacggcggc ttcgtgagcg cgctcctgat ctcccttctc gaagcgggtg
     1081 agatcgacgg cgccctgctg gcgcgcgaga gcgagacgga acgctggaag ggcgaggcgt
     1141 tcctggcgac cacacccgag gaggtgcgcg agtgcgccgg tagcttctac aaccagacgc
     1201 tcgcactcgg gcacgttgac ttcaagagct ccgacctgcc gccgaacccg aggatcgcgg
     1261 tggtcgggac gccgtgtgag atcgaaggca tcaaggcgat gcaggcgagg ccgtggacgt
     1321 ggggcagctc gaaagtcgag gccataacgc tcacgatagc tctgctgtgc accaagagct
     1381 tcaactacga aaaattgatg ctcgaggaga tccaggacaa aagaaacgtc gacctggata
     1441 acgtagggcg ggtcgacatc atccgtggga agatgatcgt ccaggacctc gacggggcca
     1501 cgatcttcga ggagccgata cgcgatttcc acggcgccgc cctgaagggt tgcgacgag
//
LOCUS       DATAIR010000630        11439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_65717, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000630 DATAIR010000000
VERSION     DATAIR010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65717"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VHM69_14910"
     CDS             complement(<1..577)
                     /locus_tag="VHM69_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2741731.1"
                     /translation="MRRIHRGISLVVLGVVSALVAVGCGGGAGSGDGGAPSVKIVSDL
                     PLQGANRAQTTTMVNAIELAIQQRDGKAGDIKIDYESFDDATAQAGQWDEAKCGENAQ
                     KAAQDEEIVGWIGPFNSGCAAVEIPSLNRAGLGMISPANTYIGLTKPGGASDEPEKYY
                     PSGERTYARVIVADDKQGKAGVALMQEEGYES"
     gene            741..824
                     /locus_tag="VHM69_14915"
     tRNA            741..824
                     /locus_tag="VHM69_14915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:Leu,seq:caa)
     gene            1004..1138
                     /locus_tag="VHM69_14920"
     CDS             1004..1138
                     /locus_tag="VHM69_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741732.1"
                     /translation="MQGGGKAVVAEDFGGNLGRDAVDAYEGGEGAGDPYAAPRRWNNL
                     "
     gene            1239..1418
                     /locus_tag="VHM69_14925"
     CDS             1239..1418
                     /locus_tag="VHM69_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741733.1"
                     /translation="MKWELDLWYELWDRAGSQGLDIRRELPYKRRAEDRQRAVPATDV
                     DRFDVMSSLHEGGPK"
     gene            complement(1697..2179)
                     /locus_tag="VHM69_14930"
     CDS             complement(1697..2179)
                     /locus_tag="VHM69_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX2741734.1"
                     /translation="MASTGIARNVDVEVRALIYRSCLRLDEGDFAGWIDLCAPEFNYS
                     ITAYSPEIRKEMVWLEHDREGMEGLFKMLPKHNSDHSPLTRHASVYTVEYDESGKEAS
                     VVTSLVVYKTQLDGGATDLFAVGKYFDTVSLEEEIPLLTNRNVRLDTRELGIGSHVPL
                     "
     gene            complement(2215..3441)
                     /locus_tag="VHM69_14935"
     CDS             complement(2215..3441)
                     /locus_tag="VHM69_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX2741735.1"
                     /translation="MGRTASEWGRKPPFPDTHYVDTLVYTDETIFEEEKERIFAKTWM
                     LACHESELPEPNDYRTYHHPAGVNLIVVRGEDGEVRSFYNVCPHRGNTILYSPSGNAK
                     RMTCIFHAWSFDYKGNCVGLPREKAGYCSGDECTITRDDLGLREVRTQVAYGGFVYVN
                     LDDECDPLEEHIGSALDTIIDQIDTEPLEVFSYHRAVVNTNYKLWHDTNSEFYHDYLH
                     VHNRLTSMKQEGYFDRKMIPFPNGHAEVGSQEVHYEAYEGGKGRADAGFPGVEGNPWS
                     LIDLFPGYSYNLRASVLRVDSQIPLAPNKTLVEYRSLGLKSDTEEQRRQRIKDNLVIW
                     GPFGRNLHEDLLAVHGQGRAISNGSRYVLHGREENSTIHDEVGMRHFYAEWSRRMGRL
                     ASDPTMKLEARKHARA"
     gene            complement(3502..4008)
                     /locus_tag="VHM69_14940"
     CDS             complement(3502..4008)
                     /locus_tag="VHM69_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741736.1"
                     /translation="MHAIRVLEEDHARIEALFERLADADDARDRDWLFERLEKELTVH
                     TLAEDNIFLPHVEEAVEDTRKATQQFFDEDALAEASWLLAAAYENNGEIRTLMEQRQA
                     ARTEELNKAVRLHVELEESLFSRAEKVLEAEDFERIGDLIEHCKWQVRGLVQARLASS
                     ASFRPSPM"
     gene            complement(4078..5199)
                     /locus_tag="VHM69_14945"
     CDS             complement(4078..5199)
                     /locus_tag="VHM69_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX2741737.1"
                     /translation="MTQSPARKTTVARRTIRKPLPPEKLVDFRKGEVEPGDPTEDLAL
                     NAEMRWEMMAAAGYLTPIDRFFVRNHAPTPRIDPASWRLRVEGPGVKRPIELGYDDLT
                     RLPSVSVVRALECAGNGRVFFEERQGQEAPGTPWRLGAIGVAKWTGVPLREILERAGL
                     KETAREVMPEGLDAVKMRRPLPLWKALEGDTILAVGMNGQLLPSDHGFPARVVVPGWA
                     AVASVKWVGRLLVSDGPLYSPWNTAKYVMTGGTWGSRRKPVAAQVPKSALELPWPATL
                     RCGRQTVTGRSWSAGAAIMRVEYAVDGDTQWRPAQLFGTNLPGAWARWRFDWQAQPGW
                     HELRVRTTDVAGNVQPETAPWNELGYMYGGVVGHPVEVL"
     gene            complement(5273..5524)
                     /locus_tag="VHM69_14950"
     CDS             complement(5273..5524)
                     /locus_tag="VHM69_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741738.1"
                     /translation="MQKQSELKNDNEGNARVTLLERLFSRESRYLPLILKLQLAFLFV
                     LVVMAFFLPTSGSERGIVVVAAGVLAGLNWAILDHVRQR"
     gene            complement(5526..5789)
                     /locus_tag="VHM69_14955"
     CDS             complement(5526..5789)
                     /locus_tag="VHM69_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741739.1"
                     /translation="MNEVGVPVEFLLGIVAFSILLIAWPEETSLPPTDMEGGLPGGVV
                     LVAVGMITRLQIRESPALNKVKQASQNVAQVPACRVPRPVVER"
     gene            complement(5786..6775)
                     /locus_tag="VHM69_14960"
     CDS             complement(5786..6775)
                     /locus_tag="VHM69_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX2741740.1"
                     /translation="MIERATSRRPRGETASVRGMVRVPGGAFLMGSNDFYPEERPVHR
                     VAVEGFWMDQHPVTNAEFHAFVEDTGYVTVAERQPDPADYPYADPSLLVPGSLVFRKP
                     RGPVDLRDYRAWWEYAPGASWHRPEGPGSTLHGREQHPVVHVAYEDALAYAAWSGKEL
                     PTEAEWEFAARGGLEGATYIWGDEFAPEGRMMANTWQGRFPWQNLKIDGYEGTSPVRA
                     FPANGYGLHDMAGNVWEWTSDSFAPHPAEQAKSSCCVPRKLQANSSKQSHITNQPGGH
                     SPRRVVKGGSYLCAPNYCLRYRPAARQGESVDTSAHHIGIRCVVRTSAVERNA"
     gene            complement(6780..8147)
                     /locus_tag="VHM69_14965"
     CDS             complement(6780..8147)
                     /locus_tag="VHM69_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX2741741.1"
                     /translation="MPEGPNIVFFHVDNLGMGELGCYGGGILRGADTKRVDQFATEGL
                     KLSHYVVEPQCTPTRSALMTGRYPIRSGNHTIALGGNQGGLVAWEQTMGDILSEAGYA
                     TACFGKWHIGAEDGRWPTDHGFDEWYGPARTYDECLWLDDPFYDPERDGYSYMYEGRK
                     GEGVRTLTDQQLTQELKGSVDAEYQRRAFDFVKRSSEADKPFFLYYNHSLMHFPMVPR
                     EEFKGASTNGDWGDCLLMLDHDFGALLDYLDELGIADNTIVIFAGDNGAEDHMIGRGT
                     AGFFDGSYFSSAEGGIRTPCLIRWPGKVVAGRESNEMVHVTDMFTTLLGLAGCEVPQD
                     RTIDGLDQSSFVLGEREESDREACIVWLNEELHAVKWHHFKISFVRQQHFHDPELPLG
                     FARIMNLLEDPKEREPVNQQYVRWWVMQHAHNTIREFESSVERESLIPPGAPLNYVPE
                     VGEPS"
     gene            complement(8398..9417)
                     /locus_tag="VHM69_14970"
     CDS             complement(8398..9417)
                     /locus_tag="VHM69_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2741742.1"
                     /translation="MHDHRGPLRPPRRLSRREFLGLSAAGAAGLALAGCGGAATGSSG
                     SGGKLRMVYQPGTTVFAQLVIMEQEGWLEKALPDYEVSWTQVDAGAAVRDAMATGEAD
                     IGAGGIGPFLVGYDGGVDWRILSALNDIEMWLMVNDDKFQSLEDFGPEDKIAVVAPDS
                     VQAVFVRKAAEEQLGDPHALDNNLVTLPSPDSVQALLSGQIAAHSTYPPFPFQEQDKG
                     ARPIVRSFDLFGTHGLMSVWVVKEFHDANPKIMETVYNNIQRATTLIQDDPERTGEIL
                     AKASQIDPAEETRFLTEEGVSFTTEPHGYIEFSEFMESADLIKDVPGSWKDLVYDNLK
                     NTNGS"
     gene            complement(9450..10322)
                     /locus_tag="VHM69_14975"
     CDS             complement(9450..10322)
                     /locus_tag="VHM69_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2741743.1"
                     /translation="MRSLNDAATTNLRPDAASSEGVPGRKNRGGYWSRLPVALRRAIL
                     VAGMVAAWQLYVSLSGVQPLVLPGPVDVAVAFWDGWTEGRLALATLVTLKTLCIGMLA
                     GTAVAFLLTVLATWTRVGEDLLSLLTSMFNPVPAIAILPLAILWFGLNPRALVFVIVY
                     SIIWPIAINLSTGFKTVNPTIMMVGRNLGLRGWRMVGDVLMPAALPHTISGLRTGWAF
                     GWRTIVAAELVFGVAGKGGGLGFFINDARYFLRIPEVFAGILTIALIGILVEVVFNAV
                     ERRTVIRWGMKSGT"
     gene            complement(10306..11115)
                     /locus_tag="VHM69_14980"
     CDS             complement(10306..11115)
                     /locus_tag="VHM69_14980"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2741744.1"
                     /translation="MLDVNDRQVRAGYPLLSVESARIGYEVDGRFNVAVGDVSFDIWP
                     REKLILLGPSGCGKSTLLKAIAGFIEPDGGTISFAGRDDLRPGPDRAVVFQEFDQLFP
                     WRTVLDNVAYPLRVTGRGKDESGETASSYVEMMGLSHAADRFPHQLSGGMKQRVAIAR
                     ALALDPLMLLMDEPFGSLDAQTRSRMQRELNEIAARTEVTILFVTHSIQEALVLGDRV
                     VVLGQPPSEVEEIVDVGHITDPDSEELVQVRRRLRELLAGEGEEVESAVFE"
     gene            complement(11103..>11439)
                     /locus_tag="VHM69_14985"
     CDS             complement(11103..>11439)
                     /locus_tag="VHM69_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741745.1"
                     /translation="LVTDMMSEERQGTTRDGEKVSAVDIYAQEMLTSIKDVVPYLFKP
                     WRGRVAVDDGYTGLSGDPCAYSQEAGVAIADAVTASICRSREAGSPVWIMQTPQATRE
                     RVRRFLCST"
BASE COUNT         1979 a         3735 c         3438 g         2287 t
ORIGIN      
        1 cggactcgta gccctcctcc tgcataaggg cgacgccagc cttaccctgc ttatcgtctg
       61 ccacgatcac acgcgcgtag gtgcgctcgc ccgaaggata gtacttctcg ggctcgtcag
      121 acgcgccacc cggcttggtc aggccgatat acgtgttcgc ggggctgatc atgccgagac
      181 ccgcccgatt gaggctcggg atctctaccg cggcgcagcc cgagttgaaa ggcccgatcc
      241 aacccacaat ctcctcgtcc tgggcggcct tctgcgcgtt ctcaccacat ttggcctcat
      301 cccactggcc ggcctgcgcc gtggcgtcgt cgaaggactc atagtcgatc tttatgtccc
      361 cggccttgcc atcccgctgc tgtatggcaa gctctatggc gttgaccatc gtcgtagtct
      421 gggccctgtt cgcaccctgc aatggcaggt cgctgactat cttcacgctc ggcgctccgc
      481 catcaccgct acctgctcca ccgccgcacc cgacggcgac cagggccgat accacgccca
      541 gcaccacaag actgatccct cggtgaattc tcctcaagtt tcctccttgt atccggagcg
      601 cgccattgat ccgccccgat gacggtccgt aaaggcctgt tgcggcggca ttatgagtat
      661 tagcactcct ctttgtcaag cgagccttga tggcatgccg aacgagccct ctacattggt
      721 ataatgtcgc agttctccgc gccggagtgg cggaattggt agacgcgccg gactcaaaat
      781 tcggttccct tcggggagtg agggttcgag tccctcctcc ggtacttctg acgaaactcc
      841 cgttgttatg ggagtttcgc tcgcgcaatc aggccccgac catggtctcc gctccgcctc
      901 ttggtgacag ctttggtgac aacaggccta ccaccaatag ataaggacag aattcgctgc
      961 gctttgcctc cgcatgcgac ctgcttgttg cgcgagcgca ggcgtgcagg gtggcggcaa
     1021 ggccgtagtc gccgaagact tcggaggcaa cctcggcaga gacgccgtcg acgcgtacga
     1081 aggtggcgaa ggggccgggg acccttacgc agcaccccgc agatggaata acctctgaaa
     1141 ggacgccagg acgcttgact ccttatttcc cttcgtttac cctgtttgtg catttataat
     1201 ataggcaata tggagaatat gaaaaggagg tagcggcgat gaagtgggag ctggatctct
     1261 ggtacgagct ttgggacagg gcgggtagcc agggtttgga catccggcgt gagcttccgt
     1321 ataagaggcg ggccgaagac aggcagcgcg cagttccggc tacggatgtc gacaggttcg
     1381 acgtgatgtc ctccctacac gaaggtggtc cgaaatgagg gtgggtgcac gggtgccaag
     1441 cgatagaaca ggcgaatcgg cgaccggcgt ggcagtaaga agcgaatcag aggggcaggt
     1501 tcgcatcggc accgcgcctg caccagcacg aggccggcaa cctcggggcc cggcggagga
     1561 gcgacactat tcgtggctcc aggatcatct cgcgcgtctc gacggcaaag aggcgcgcag
     1621 tgagtactac gagaggctgg ctgggtaagc ccctgcgaag ggcctggtat gtgcgtgaga
     1681 ggttatggac cgtagcctac aggggcacgt gcgagccgat gccaagttcc cgcgtgtcta
     1741 gcctgacgtt acggttcgtg aggagaggaa tttcttcctc aaggctcacc gtgtcaaagt
     1801 actttccgac ggcgaagagg tcggtagcgc ccccgtccag ttgggtcttg taaacgacca
     1861 atgaggtgac gacggatgct tcctttcccg attcgtcgta ctcgacggtg tagacgctcg
     1921 catggcgggt gaggggagag tggtcgctat tgtgcttcgg cagcatcttg aagaggccct
     1981 ccataccctc tcggtcgtgc tcaagccaga ccatctcctt gcggatctcc gggctgtagg
     2041 ccgtgatact gtagttgaac tcaggggcgc agaggtcgat ccaccccgcg aaatcccctt
     2101 cgtccaggcg cagacaggag cggtagatca aagctctcac ctccacgtct acgttccttg
     2161 cgattccggt gctcgccatg tctttctcct cggttggctt gtgggctctt atctttaggc
     2221 tctcgcatgc tttctggctt ccagcttcat tgttgggtcg ctcgccaggc gtcccatacg
     2281 cctgctccat tcggcgtaga aatggcgcat gccgacctcg tcgtggatcg tactgttctc
     2341 ctcgcggccg tgtaggacgt agcgcgagcc gttcgagatc gcgcgcccct ggccgtggac
     2401 ggcgagcagg tcctcgtgca ggttgcggcc gaagggaccc cagatcacca ggttgtcctt
     2461 gatcctctgg cggcgctgct cttctgtgtc gctcttgagg cccaaggaac ggtactcgac
     2521 taaggtcttg ttcggggcga gcggtatctg cgagtcgacg cgcaggaccg aggccctcag
     2581 attgtaggag tagccgggga agaggtcgat cagggaccag gggtttccct ccacacccgg
     2641 aaatccggcg tcggccctgc ccttgccgcc ctcataggct tcgtagtgga cctcctggga
     2701 gccgacttcg gcgtggccgt tcggaaaggg gatcatcttc cggtcgaagt acccttcctg
     2761 cttcatgctc gtaagccggt tatggacgtg cagatagtca tggtagaact cgctgttggt
     2821 gtcgtgccag agcttgtagt tggtgttgac cacggccctg tgatacgaga agacttccag
     2881 cggctctgta tctatctgat caatgatggt gtcgagcgcg gaaccgatgt gctcttccag
     2941 agggtcgcac tcgtcgtcga ggttgacgta gacgaagccc ccgtatgcta cctgcgtcct
     3001 cacctcgcgc agtccgaggt cgtccctggt tatcgtgcac tcgtcgcccg agcagtagcc
     3061 ggccttctcg cgcggaagac cgacgcagtt tcccttgtag tcgaaggacc aggcgtggaa
     3121 gatacaggtc atgcgcttgg cgttgcctga cggactgtac aggatcgtgt tccccctgtg
     3181 cgggcagacg ttgtagaacg aacggacttc tccgtcctcg ccccgtacca cgatgaggtt
     3241 tacgccagca ggatgatggt aggtgcggta atcgtttggc tccgggagct cgctctcgtg
     3301 gcaagcgagc atccacgttt tcgcgaagat cctctccttc tcttcctcga agatcgtctc
     3361 gtccgtgtag acgagggtgt ccacgtagtg cgtatcaggg aagggaggct ttcgtcccca
     3421 ctcgcttgcg gtgcgtccca tccttctttc ctccttactc gttggtcagt acagggttca
     3481 cttcgtgctc ctcactcgcg cttacatcgg tgatgggcgg aagctcgcag aggaggccag
     3541 cctcgcctgg accagccccc ttacctgcca cttgcagtgt tcgatgaggt ccccgatcct
     3601 ctcgaagtct tccgcctcga ggaccttctc ggccctggag aatagactct cctccagctc
     3661 gacgtggagc ctcacagcct tattcagctc ttccgttcgg gctgcctgcc gctgttccat
     3721 gagggtccgg atctccccgt tgttctcgta agctgcagcc agaagccagg acgcttccgc
     3781 aagggcgtct tcgtcgaaga actgctgcgt tgcctttctc gtgtcctcga cggcctcctc
     3841 tacgtgcggg aggaagatat tgtcctcagc gagggtgtga acggtgagct ccttttcgag
     3901 ccgctcgaac agccaatccc tgtcccgggc atcgtccgca tcggccaacc gttcgaacag
     3961 ggcctcgatc cgggcgtgat cctcctctaa caccctgata gcgtgcacta ttacctcccc
     4021 agtagcgtgc gcaggcgatc ccataccttg tgcggttttc cgcatgcatc gagtccctca
     4081 aagcacctca acagggtgtc ccacgacgcc gccgtacatg tatccgagct cgttccacgg
     4141 cgccgtctcc ggctggacgt tgcccgcgac gtccgtcgtt ctaacgcgca gctcgtgcca
     4201 acccggctgc gcctgccaat cgaacctcca tcgcgcccac gcccctggca ggttggtccc
     4261 gaagagctgc gccggacgcc actgcgtatc gccgtccaca gcgtactcaa cccgcatgat
     4321 ggcggcgcca gccgaccacg agcgtcctgt tacagtctgc ctcccgcacc gcagcgtggc
     4381 cggccacggc agctcgaggg cgctctttgg cacctgagcg gcgacgggtt tccgtcgcga
     4441 accccacgta ccgcccgtca tgacgtactt cgctgtgttc catggcgagt acagcggtcc
     4501 atcggacacc agcaggcggc cgacccattt cacgctcgcc accgccgccc acccgggcac
     4561 caccacgcgc gcagggaagc cgtggtccga cgggagcagt tgcccgttca tgcctactgc
     4621 gaggatcgtg tccccttcga gggccttcca cagaggcagc gggcggcgca tcttaaccgc
     4681 gtcgagaccc tccggcatca cctcccgggc cgtctccttg aggccggcgc gctccaggat
     4741 ctcgcgcagc ggcaccccgg tccacttggc gaccccgatc gcaccgagcc tccacggtgt
     4801 tcccggggcc tcctggccct gccgctcctc gaagaatacc cgcccgttcc ccgcgcactc
     4861 cagcgcccgc accaccgaga cgctcggcag ccgcgtgagg tcgtcgtagc cgagctcgat
     4921 gggacgcttc acgcccggac cctcgacgcg cagccgccag cttgccgggt ctatgcgcgg
     4981 ggtcggggcg tggttgcgga cgaagaagcg gtcgatgggc gtaaggtaac cggcggctgc
     5041 catcatctcc cagcgcatct ccgcgttgag ggcgaggtcc tcggtcgggt ccccaggttc
     5101 cacctcgcct ttgcggaagt cgacgagctt ctcagggggc aacggcttgc ggatggtcct
     5161 tctcgcaaca gttgtcttcc tggccgggct ctgtgtcatg ccccaccccc aacctcgcca
     5221 ccagggcacg caaacctcga acagagccct cggcgcgtcg cgacgaccga gctcatctct
     5281 gcctcacgtg gtccagaatg gcccagttca agccagccaa cacacctgcg gccacgacga
     5341 cgatccccct ctccgagcca gaggtcggca ggaagaaggc cattaccacc agcacgaaca
     5401 ggaacgccaa ttgcagcttg aggatcaacg ggaggtacct gctctccctt gaaaacagac
     5461 gctccagcag cgtcactcgc gcgttacctt cgttgtcatt cttgagttca gattgcttct
     5521 gcacgctaac gctccacgac cggcctgggt acccggcatg ctggcacctg ggccacattt
     5581 tgtgacgctt gcttgacctt gttgagtgcc gggctctccc taatctgcaa ccgtgtgatc
     5641 atgccaaccg ccacgagaac aacgccgcca ggaaggccgc cctccatatc cgtaggaggg
     5701 aggctggtct cttcgggcca cgctataagc aggatgctga aagcaacgat ccccagcaag
     5761 aactccaccg gcacccctac ctcgttcatg cgtttctctc tactgctgac gtgcgcacga
     5821 cgcatcggat acctatgtgg tgtgcggatg tgtccactga ctcgccctgc ctcgccgccg
     5881 gacggtagcg caggcagtaa ttgggcgcgc agaggtatga accgcccttc accacccttc
     5941 gcggactgtg tcctcccggc tgattcgtaa tgtggctctg tttcgaggag ttggcctgga
     6001 gtttgcgcgg aacgcagcag gatgattttg cctgctctgc aggatgcggc gcgaaggagt
     6061 ccgacgtcca ctcccagacg ttaccggcca tatcgtgtag tccataaccg ttggcgggaa
     6121 aggctctgac cggagaggtg ccctcgtacc cgtctatctt caggttctgc caggggaatc
     6181 ggccttgcca ggtgttggcc atcatccgcc cctcaggtgc gaactcgtcg ccccagatgt
     6241 aggttgcgcc ctcgagcccg ccgcgggcgg cgaattccca ctcggcctct gtcgggagct
     6301 cctttccaga ccaggcggcg tatgccaggg cgtcctcgta agcgacgtgt accaccgggt
     6361 gctgttcccg gccgtggagg gtactgcccg gtccctccgg ccggtgccag ctcgcgccgg
     6421 gcgcgtactc ccaccaggcg cggtagtcgc gaagatccac gggcccgcgc ggtttcctga
     6481 acaccagcga accaggcacc agcagggatg ggtcggcgta cgggtaatca gcggggtcgg
     6541 gctggcgctc ggcgacggtg acgtatccgg tgtcctccac aaatgcgtga aactcggcgt
     6601 tggtcaccgg atgctgatcc atccagaaac cttcgacagc gacgcggtgg accgggcgct
     6661 cctcggggta gaagtcgttc gatcccatca ggaaggcacc gccgggcacc cggaccatgc
     6721 ccctcacgct cgctgtctca ccgcgcggtc ttcggctggt ggctctctcg atcataatct
     6781 tacgagggct ccccgacttc cggcacgtag ttgagtggcg ccccaggggg gatcagcgac
     6841 tctctctcga cgcttgactc gaactccctg atggtattgt gagcgtgctg catcacccac
     6901 cagcgcacgt actgctggtt gacgggttct cgttcttttg gatcctccag gagattcatg
     6961 atgcgcgcga agcccagcgg caactccggg tcgtggaagt gctgctgtcg tacgaagctg
     7021 atcttgaaat ggtgccattt gaccgcgtgc aactcctcgt tgagccacac gatgcaggcc
     7081 tcgcggtcgg actcttcccg ctcgccgagc acgaaggagc tttggtccag gccgtcgatg
     7141 gtccggtctt gcggtacctc gcacccggcc aatcccagca gcgtggtgaa catgtcggtg
     7201 acgtggacca tctcgttgct ctcgcgtccc gccacaacct tgccgggcca cctgatcaaa
     7261 cacggcgtac ggatgccccc ctccgccgag ctaaagtagg agccgtcgaa gaaacccgcc
     7321 gttcctcgcc cgatcatatg gtcctcggct ccgttgtcgc cggcaaagat aacgatggtg
     7381 ttgtcggcta tccccaattc gtccagatag tcgagcaacg cgccgaagtc gtggtccagc
     7441 atcagcaggc aatcgcccca gtcgccgttc gtactggccc ccttgaactc ctcgcgtggg
     7501 accatcggga agtgcatcag cgagtggttg tagtagagga agaacggttt gtccgcctcc
     7561 gagctgcgct tgacgaagtc gaacgcgcgc ctttgatact cggcgtccac actccccttc
     7621 aactcctggg tgagttgttg atccgtgagc gttcgcacgc cttcgccctt gcggccctcg
     7681 tacatgtaag agtacccgtc gcgctcagga tcgtagaaag ggtcatcgag ccagagacat
     7741 tcgtcgtagg tacgggccgg gccgtaccac tcgtcgaagc cgtggtcggt cggccaacgc
     7801 ccatcctcgg ccccaatgtg ccactttccg aagcacgccg tggcgtagcc ggcctccgag
     7861 aggatgtcgc ccatggtctg ctcccatgcc accaggccac cctgattgcc accgagggcg
     7921 atcgtgtgat tgccagatcg gatggggtaa cgtcccgtca tcaaagccga acgggtgggc
     7981 gtgcactgag gctcgacgac gtagtgcgaa agcttcaagc cttctgtggc gaactgatct
     8041 acgcgcttcg tgtcggcccc tcgcaagatg ccgccgccgt agcagcccag ctcgcccata
     8101 cccagattat cgacgtggaa gaagacgatg ttcggtcctt caggcaaatc gcacctcctg
     8161 ttgtacattg gtaatcgcta tgctcgccaa tctcgggtca cggaagctcc gcatcgagca
     8221 cgtggggatg agcgcgccca agatcttgac ctcgaccgcg ctccggacct ggttgccaag
     8281 cttcatcctc gcgccgacga gcttcccgcg cgaacggatc tgggtcggat gcaaaaccct
     8341 ccgcacagta tctgatcgga gggggcaacc aggtctagtt tccccaaaac tcatgtctca
     8401 gcttccgttt gtgttcttga ggttgtcgta gacgaggtcc ttccaggagc cgggcacgtc
     8461 cttgatgaga tcggcgctct ccatgaactc ggagaactcg atgtacccgt gcggctcggt
     8521 agtgaacgag acgccctcct cagtgaggaa cctcgtttcc tcggcggggt ctatctggga
     8581 ggccttggcg aggatctcgc cggtcctctc ggggtcatcc tggatgagcg tcgtggccct
     8641 ctggatattg ttgtagacag tttccatgat cttcgggttg gcgtcgtgga actccttgac
     8701 gacccagacg ctcatgaggc cgtgcgtgcc gaagaggtcg aagcttctga cgatgggccg
     8761 ggcgccctta tcctgctcct ggaacgggaa cggcggatag gtggagtggg cggcgatctg
     8821 gccgctcaag agcgcctgca ccgagtcggg gctgggcagg gtgacgaggt tgttgtcgag
     8881 cgcatggggg tcgccgagtt gctcctccgc cgccttgcgg acaaagacgg cctgtactga
     8941 gtccggggcg accacggcga tcttgtcttc ggggccgaag tcttcgaggc tctggaactt
     9001 atcgtcgttg accatcagcc acatctcgat gtcgttgagg gcgctgagga tcctccagtc
     9061 tacgccgccg tcgtagccga ccaggaaagg gcctatcccg ccggcaccta tgtccgcctc
     9121 accggtagcc atcgcatcgc gcaccgctgc tccggcgtcc acctgggtcc atgagacctc
     9181 gtagtccggc agggctttct ccagccagcc ttcctgttcc atgattacca gctgcgcgaa
     9241 gacggtggtc cccggctggt agaccattct cagcttcccg ccgctcccac tgcttccggt
     9301 tgctgccccg ccgcacccgg ccagcgcgag ccccgctgcc ccggcggcac tcaagcccaa
     9361 gaactctcgc cggctaaggc gtctcggcgg acgcaatggt cccctatggt cgtgcattgg
     9421 tgcctccttt tcggtaagtt gggttttgtt caggtgccgg acttcatgcc ccatcggatc
     9481 acggtgcgcc gttccacggc gttgaagacc acctccacta gtatcccgat cagggctatg
     9541 gtcaggatgc cggcgaagac ctcgggtatc cgcaagaagt agcgggcgtc gttgatgaag
     9601 aagcccagtc ccccaccctt tccggccacg ccgaagacca gctcggcggc gacgatggtg
     9661 cgccagccga aggcccagcc ggtcctgagg cccgagatgg tgtgtggcaa cgcggcgggc
     9721 atgagcacgt cgccgaccat ccgccagccc cgcagtccca ggttgcgtcc gaccatcatg
     9781 atggtggggt ttaccgtctt gaagcccgta ctgaggttta tggcgatggg ccagatgatc
     9841 gagtacacga tcacgaagac gagcgcccgg ggattgaggc cgaaccatag gatggcgagc
     9901 ggcaggatgg cgatggcagg gacagggttg aacatggagg tgagcagcga gagcaggtcc
     9961 tcgccgaccc gcgtccacgt cgcgagcacg gtgagcaaga acgcaacggc cgtcccggcc
    10021 agcatcccga tgcacagggt cttgagcgtt accagggtcg cgagggccag ccggccttcg
    10081 gtccagccgt cccagaaggc caccgccacg tccacgggac cggggagcac cagcggctgt
    10141 acgcccgaga ggctcacgta gagctgccag gcggccacca tcccggctac caggatcgcg
    10201 cgcctcaggg cgaccggcag ccgcgaccag tacccgccgc ggttcttgcg tccaggaaca
    10261 ccctccgatg aggcggcgtc aggccgaaga ttcgtcgtgg ctgcgtcatt caaagaccgc
    10321 actctccacc tcctctcctt cgccggccag caactccctt agccgccgcc gcacctgcac
    10381 tagttcctcg gagtcggggt ccgttatgtg gccgacatcc acgatctcct cgacctctga
    10441 gggaggctga ccgagcacga cgacgcggtc cccgagcacc agcgcctcct ggatactgtg
    10501 cgtgacgaag agtatcgtca cctcggtgcg ggcggcgatc tcgttgagct cccgctgcat
    10561 acggctgcgt gtctgcgcat cgaggctgcc gaaaggctcg tccatgagca gcatcagcgg
    10621 gtccagggcc agggctcgcg ctatggcaac ccgctgcttc atcccgccgg agagctggtg
    10681 cgggaaacgg tcggccgcgt gcgagaggcc catcatctcc acgtagctgc tggcggtctc
    10741 ccccgactcg tccttacctc gcccggtcac ccgaagcggg taggcgacgt tgtccagcac
    10801 ggtgcgccag gggaagagct ggtcgaactc ctggaataca acagcccggt cggggccagg
    10861 ccgcaggtcg tctcggccgg cgaaggagat cgtgccgccg tccggctcga tgaatccggc
    10921 tatcgccttg agcagcgtgg actttccgca tccgctcgga ccgagcagga tcagcttctc
    10981 cctcggccag atgtcgaagc tcacgtcgcc gaccgcgacg ttgaaccgcc cgtcaacctc
    11041 gtagcctatc cgcgcgctct ctaccgacag cagcggataa ccggcgcgca cttgtctgtc
    11101 gtttacgtcg agcatagaaa cctccgtaca cgttctcttg tagcttgtgg ggtttgcatg
    11161 atccacactg gagaacccgc ctctcggctc ctacagattg acgcggtcac cgcatcggct
    11221 atcgctacgc ccgcctcctg cgaatacgca cacgggtctc cagagagccc cgtgtagccg
    11281 tcgtcgacgg ctacacgccc tcgccacggc ttaaaaaggt acgggacgac gtccttgatc
    11341 gaggtcaaca tctcctgtgc gtagatatct accgccgaga ccttctcccc gtcccgagtc
    11401 gtcccctgac gctcctcgct catcatgtcg gtcaccaag
//
LOCUS       DATAIR010000631         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_413001, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000631 DATAIR010000000
VERSION     DATAIR010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413001"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1485)
                     /gene="gatA"
                     /locus_tag="VHM69_14990"
     CDS             complement(40..1485)
                     /gene="gatA"
                     /locus_tag="VHM69_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX2741746.1"
                     /translation="MAGSLLDLTAYEVAKQIEAGEVSTREAAEIANARVDEVEDEVNS
                     FVTTTPELALERAERVDAKNGDRRLWEGVPLVVKDVLSTRGVRTTCGSKILENFEPLY
                     DASALLNFGEDLVMVGKANCDEFAMGSSTENSAYGPTRNPWDLDRVPGGSSGGSAAAV
                     AAGEGWWALGTDTGGSVRQPAALCGIVGLKPTYGRVSRHGLIAFASSLDVVGPMTRDV
                     RDAALLLQAIAGHDPSDSTSANVEVPNYLANLDDGVEGLRIGVVPELIGEGIEPGVRE
                     VVERVARRLESAGAEVGEASLPHSGYGLPAYYIIAPAEVSSNLARFDGVRYGLRVPAQ
                     GVHEMYRKTREAGFGDEAKRRIMLGTYALSSGYYDAYYAQAQKVRTRIIEDFRAAFAR
                     YDVLLSPTAPSVAFRIGEKVDDPLAMYANDICAVPANLAGIPAISVPGGLSEGLPVGV
                     QLMGDHFTEPTLLRAARAAEEASDFEFTLKP"
     gene            complement(1487..1768)
                     /gene="gatC"
                     /locus_tag="VHM69_14995"
     CDS             complement(1487..1768)
                     /gene="gatC"
                     /locus_tag="VHM69_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2741747.1"
                     /translation="MISEEQVRHVANLARLGLTDDEIEKMGTQLDAILDSIEKIRELD
                     LTDVPPTANPLNLSNVLRPDEPRPELSQDEALSPAPERVDDLFAVPRID"
     gene            complement(1803..2003)
                     /locus_tag="VHM69_15000"
     CDS             complement(1803..2003)
                     /locus_tag="VHM69_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741748.1"
                     /translation="MEHSGRHAARCVAYMVEHLRPLQPVWLAEQKNQPSLELAARLGF
                     VPVDEIIVFHPPQGQDIDPALR"
     gene            complement(2134..2505)
                     /locus_tag="VHM69_15005"
     CDS             complement(2134..2505)
                     /locus_tag="VHM69_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741749.1"
                     /translation="MSDESTLAGALPDIPRWVEARSTLLGGCELFGLDEEGEEPSFIV
                     RDAVYPLVSVVGCPSLQAIREPVAGRRGYDVLVPPEKGDHVAAALPAWERSPATLHEL
                     GDEGRSLSSRQTLCDCWNLRS"
     gene            complement(2495..3355)
                     /gene="folD"
                     /locus_tag="VHM69_15010"
     CDS             complement(2495..3355)
                     /gene="folD"
                     /locus_tag="VHM69_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX2741750.1"
                     /translation="MAKILDGKAAAAEVRLEVASEVAQLEERGVPVRLDVILVGEDPA
                     SVTYVASKERDCREVGIESRVHRFPAQVSQAELADLVDRLNEDTAVSGFFIQLPLPGG
                     LDAMLLLSRIAPSKDVDGLSPESAGRLAVGLPSLLPCTPHGIIQLLNRNGVDLAGREA
                     VVVGRSNLVGKPLSQLLLRENATVTVCHSRTRNLIDVTRRADVLVVAAGQREMVGAEH
                     VREGAVVVDVGIHRKEEGGLTGDVRFEEVEPVASYITPVPGGVGPMTRAMLLHNAVRA
                     AQIAEGGLVR"
     gene            complement(3363..>4244)
                     /locus_tag="VHM69_15015"
     CDS             complement(3363..>4244)
                     /locus_tag="VHM69_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HEX2741751.1"
                     /translation="WEVMSENPLTVNPDDLISDITEQVMEVHYRAAIAVDDKYVPIGV
                     ITRTDLLNPKPRRVLLVDHAEVGQSVKGVEDAEVVEILDHHHVGDIETTSPILATFDP
                     VGSTATLIVERFKGSGLRPEESTAKMLLAALLSDTVILNSPTTTDRDREVAEYLEQML
                     DLDAEEFGTEMFEASSDVSSLSAKEIVNRDAKEYGTSSGEKVSVSQVETVGSGLLERK
                     DELIDALEVLKGENGYLFSALMVTDIMERGTQLLCVGDCTPVHNAFDGQAENGVIDLP
                     GVMSRKKQVAPVLLAVL"
BASE COUNT          722 a         1374 c         1384 g          764 t
ORIGIN      
        1 taacgagaag acagcagtcc cgcctgcaag ccaggttctc tagggcttga gcgtgaactc
       61 gaagtccgat gcctcttcgg cggcgcgggc cgcgcgcagc agcgttggtt ctgtgaagtg
      121 gtcgcccatg agctggacgc ctacggggag accttccgag aggccgccgg ggacgcttat
      181 ggcagggatg cccgcgaggt tcgcggggac cgcgcagatg tcgtttgcgt acatggcgag
      241 ggggtcgtcc accttctccc cgatcctgaa cgccacagag ggggctgtgg gcgagagtag
      301 cacgtcgtag cgggcgaagg ccgccctgaa gtcctcgatg atcctggtac gaactttctg
      361 cgcctgggcg tagtaggcgt cgtagtagcc ggaagacagg gcgtaggtgc cgagcatgat
      421 ccttcgcttc gcctcgtcac cgaagcctgc ctcacgcgtc ttgcggtaca tctcgtgcac
      481 gccttgagca gggaccctga gaccgtagcg tacgccgtcg aagcgggcga ggttggagga
      541 aacctcggcc ggggcgatga tgtagtaggc cgggaggccg tacccggaat gcggaaggct
      601 tgcctccccc acctcggccc cggcgctttc gagccggcgg gcaaccctct cgaccacctc
      661 acgcacgcct ggttctatcc cctcgccaat caactcgggc acaaccccga tgcgcagtcc
      721 ctccacaccg tcgtcgaggt tcgcgaggta gttggggact tcgacgttgg cgctcgtcga
      781 gtcgctgggg tcgtggcctg cgatggcctg caggagcagc gcagcgtccc gcacgtcgcg
      841 ggtcatcggg ccgaccacgt cgagtgagga ggcgaaggcg atcaaaccgt ggcgcgagac
      901 ccgcccgtag gtgggcttga gcccgacgat gccgcacaag gcagcgggct ggcgcacgga
      961 gccgcccgtg tccgtcccga gcgcccacca tccttcacca gcggcgacgg ccgcggcaga
     1021 gccgccggag gagccgcccg gcacgcggtc gaggtcccaa ggattacgcg ttggcccgta
     1081 ggccgagttc tccgtcgagg agcccatcgc gaactcgtcg cagttggctt ttcccaccat
     1141 tacgaggtcc tcaccgaagt tcagcagagc cgaggcgtcg tagagaggct cgaagttctc
     1201 cagtatcttt gatccgcacg tcgtcctcac cccgcgcgta gagagtacgt ccttgacgac
     1261 cagaggtacg ccctcccaga gacggcggtc gccgttcttc gcgtctacac gctcggcgcg
     1321 ttcgagcgcc agttcagggg tcgtggtcac gaaagagttg acctcgtcct cgacctcatc
     1381 gacgcgtgcg ttggcgatct ctgcggcttc acgggtcgag acctcgccgg cttctatctg
     1441 tttcgcaacc tcataagccg tgaggtcgag caggctaccg gccatctcag tcgatcctcg
     1501 ggacggcgaa gaggtcgtcc acgcgctcgg gtgccgggga gagagcctca tcctgcgaga
     1561 gctcggggcg cggctcgtcg gggcggagga cgttcgagag gttcagcggg ttcgcggtgg
     1621 gcggcacgtc tgtgaggtcg agctcccgga tcttctctat gctgtcgagg atcgcatcga
     1681 gctgagtacc catcttctct atctcatcgt ccgtcaggcc aagccgtgcc agattggcca
     1741 cgtggcgcac ctgctcctcg gatatcattt tcagcctccg tcttcgctcg caaagctcga
     1801 acttatctta gcgcagggtc aatgtcctgg ccctgcggag gatggaacac gatgatctcg
     1861 tctacgggta cgaaccccag cctggcggcc aactccagcg atggctggtt cttctgctca
     1921 gccaaccaga cgggctgcaa gggacgcagg tgctcgacca tgtacgcgac gcaccgggca
     1981 gcgtgtctac cgctatgttc cacaggctct cggtgcgggc cgcgtagcag aacgcgaccg
     2041 ggcgcccgtc ctcgaagctc gccgccacgg gagatctgcg tccggccagg tccagctcgg
     2101 agcggaggcc gggcggcaac ccctcaacgg atttcaactc cgcaggttcc agcagtcgca
     2161 cagagtctgc cgggacgaga gggagcgtcc ctcgtcgccg agctcgtgca aagtcgccgg
     2221 agacctctcc caggccggca gcgcggcggc gacgtgatcc cctttctcgg gcgggacgag
     2281 aacgtcgtag ccccgccgac cggcaacggg ttcgcggatc gcctgcagag atgggcaccc
     2341 gacgacggag accagagggt acacagcgtc gcggacgatg aatgacggct cctctccttc
     2401 ctcatcgagg ccgaagagct cacacccgcc gagtaacgta gagcgcgcct cgacccagcg
     2461 tgggatgtca ggaagggcgc cggcaagggt ggactcatct gacaagcccg ccctccgcaa
     2521 tctgcgccgc cctgacggcg ttgtgtagga gcatggcgcg ggtcatcggg ccgacgcctc
     2581 cagggaccgg cgtgatgtag gacgccaccg gctcgacctc ctcgaagcgc acatcgcctg
     2641 tgagaccacc ttcttctttg cgatgtatgc cgacatctac tacaacggcg ccctccctga
     2701 cgtgctcagc cccgaccatc tccctctggc cggctgcgac gacgagcacg tcggccctcc
     2761 gggtgacgtc gatcagatta cgcgtgcgag agtggcagac cgtgacggtc gcgttctcgc
     2821 gcaggagtag ctgagagagc ggcttgccaa cgaggttcga gcgcccgacg actacggcct
     2881 cgcgtccggc gagatccacg ccgtttctat tgaggagctg gatgatgccg tgcggggtgc
     2941 atggcaggag gctcggcagg ccgacggcga gccttcctgc gctctctggg ctcaggccgt
     3001 ccacgtcctt cgaaggggct atgcgcgaaa ggaggagcat ggcatcaagc cccccgggaa
     3061 gggggagctg gatgaagaag ccagagacgg cggtgtcctc gttcagccgg tcgacgaggt
     3121 ctgcgagctc tgcctgagag acttgcgcgg ggaagcggtg gaccctcgac tctatcccga
     3181 cctcacggca gtcgcgctct ttagaggcga cgtaggtaac agaagccggg tcttcgccga
     3241 ccaggatcac gtcgagccgc acgggcacgc cccgttcctc cagctgcgct acctcggagg
     3301 ctacctcaag gcgtacttcg gcggcggccg ccttgccgtc cagaatcttc gccacgaagg
     3361 tcctagagga ccgcgaggag tacgggggcg acctgcttct tacggctcat cacgccgggc
     3421 agatctatga cgccgttctc ggcctgtccg tcgaaggcat tatgcacggg ggtgcagtct
     3481 ccgacgcaca acagctgcgt gccgcgctcc atgatatccg tgaccatcag cgccgagaag
     3541 aggtagccgt tctctccttt caggacctcg agggcgtcga tcagttcgtc cttacgctcg
     3601 aggaggccag aaccgaccgt ctctacctgg gagacgctca ccttttcgcc cgagctcgtc
     3661 ccgtactctt tcgcgtcgcg attgactatc tcttttgccg agagcgacga gacgtccgaa
     3721 gaggcctcga acatctccgt tccgaactcc tcagcatcca ggtcgagcat ctgctcgagg
     3781 tattcagcga cctcgcggtc gcggtccgtt gtggtcgggg agttcaggat caccgtgtcc
     3841 gagaggaggg ccgcgagcaa catcttcgct gtcgactcct cgggtctcag gccgctcccc
     3901 ttgaagcgct caacgatcag cgtagccgtc gagccgacgg ggtcgaaagt ggccaggatg
     3961 ggcgaggtcg tctcgatgtc tccgacgtgg tggtgatcga ggatctcgac tacctcggcg
     4021 tcctcgacgc ccttgacgct ctgccccacc tcggcgtggt caacgaggag aactcgcctc
     4081 ggtttgggat tgaggaggtc cgtcctggtg atgaccccga taggcacata tttgtcgtcc
     4141 acggcgatcg ccgccctgta gtggacctcc atcacctgct cggtgatatc cgagatgagg
     4201 tcgtcggggt tgacggtcag agggttctcg ctcatgacct ccca
//
LOCUS       DATAIR010000632         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_320575, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000632 DATAIR010000000
VERSION     DATAIR010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320575"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VHM69_15020"
     CDS             complement(<1..1131)
                     /locus_tag="VHM69_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX2741752.1"
                     /translation="MIVHMMGNAHIDPVWLWSWQAGVDEALATFRSAADRCDEYPEFV
                     FTRGESWLFEQVERIDPGLFERVRRQVESGQWHITGGQFIQPDVNLSTVMGLHRQIIH
                     GQRYFKDRFGVRPKVGYNVDSFGHPATMPDVLDSHGYVGYVFHRPGAHQVELPAQTFR
                     WRGANGGEVIGFRIAPAYVTFSDDLYGQIMLSIEAADPELGHTMCFYGVGNHGGGPTK
                     ANIEYILENVHSFPDAELRFSTPQAFFDAIAESTDRLPVVTEELQHTFPGCYSVMHDI
                     KQQQQRGEHLLDQCGRLVDGFVYDEVERRELYKRLDAAWDDLLFTEFHDVLAGTSIPA
                     AWDSIRAMQGRARIIGEEVAVESTRRWARRKLPPLDEQQIVVI"
     gene            complement(1206..2051)
                     /locus_tag="VHM69_15025"
     CDS             complement(1206..2051)
                     /locus_tag="VHM69_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2741753.1"
                     /translation="MRSRKARGRIGDVLTYLVFAATLVFFGGPLLWVLSLSIRTPQEV
                     YITSLRLIPENPTLDNYVEVLYTAQFSVFLINSLKLSVAGGIGAMLVAAPAAYAFSRL
                     AFRGNNGLLIGVLALQMISPLVIGIPLYRYFARLNILDSHVSTAMVYIAILVPIATWM
                     LKGFFDGIPPALDEAAMVDGCTRFGAFRRVTLPLILPGLTSAFVLTAILAWGEFTIPY
                     ILLSKPSLLPISIGILNFQGNYAQTSQQVVAAGGILAMLPAIATFVILQRFIVRALTS
                     GAIKG"
     gene            complement(2048..2953)
                     /locus_tag="VHM69_15030"
     CDS             complement(2048..2953)
                     /locus_tag="VHM69_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2741754.1"
                     /translation="MTKKAFAPRELIGENRPTLWMAPLLAVVTLFYLYPAFEVLRFSL
                     TDASLLKPDYEYTLDTFKNVLSDPVLYKVLRITAIFVLASIVLQLALGLLIAVAVNRA
                     RRRRLPGMVFMRSVILSAWVMPGVVIGIVWAIIFNEASYGLANLVLAPVGLDGMAWLS
                     DPNLALMSIVIANVWRGTAFSMILQYAGLQSIPDELYEAAEVDGASTLQSFWYITIPQ
                     LRPMLLINIILITIQTLNSFDMVLALTGGGPGQATEVLALHTYNVIFREYSLAGGAVL
                     AVIMLLISLVITLGYRRLLRSEGVV"
     gene            complement(2965..>3870)
                     /locus_tag="VHM69_15035"
     CDS             complement(2965..>3870)
                     /locus_tag="VHM69_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2741755.1"
                     /translation="LFPFVRDIVVGDDGKMYAYWFATDGRILYRRTDLVPEAPRTWDE
                     LIEAANAAEEKDSKVDGFLFNGGRWEGTTFDNLAHFWSQGGELVDEQGKPIFGEEPNR
                     TYMLNEFNFLKETVDSGASPQRVATIKDYAEFEAAAQAQQVAMFQGGDFQYPTLKDTL
                     PPEEFKKWEVSLLPTMSASDTPSSGSGGWTIGSFSDDPKKVEMCMEFTKDVFVGGMNE
                     VTGQLPTSPKLFDEIDKFDEPIYDDFRTMLQHGKARPGVPVYPTMSEELQVAIGTVLT
                     EEATPEEALDTAYKASLSAYEEQKK"
BASE COUNT          738 a         1189 c         1207 g          736 t
ORIGIN      
        1 gatgaccacg atctgctgct cgtcgagcgg cggcagttta cgacgcgccc agcgccgggt
       61 ggactccacg gccacttctt ctccgattat ccttgcccgg ccctgcatcg ccctgatgga
      121 atcccacgcc gcggggatcg aggtgcccgc cagaacgtcg tgaaactcgg tgaacaacaa
      181 atcgtcccag gcggcatcga gtcgcttgta gagttcccgt ctctccactt cgtcgtacac
      241 gaaaccatcg acgagacgcc cgcactggtc caggaggtgc tcaccgcgct gttgttgctg
      301 cttgatgtcg tgcatcacgc tgtaacagcc ggggaaggtg tgctgcaact cttcggttac
      361 gacagggaga cggtctgtac tctccgcgat cgcgtcgaag aaggcctggg gtgtactgaa
      421 ccttagttcc gcgtccggga atgagtggac gttctcgagg atgtactcga tgttcgcttt
      481 cgtaggtccg ccgccgtggt tgccgacgcc gtagaagcac atcgtgtgtc cgagttcagg
      541 gtcagccgcc tcgatggaga gcatgatctg accgtacagg tcgtcagaga acgtgacgta
      601 ggcgggagcg atgcggaacc cgatcacctc ccccccattt gcgccacgcc agcggaatgt
      661 ctgagctggc aactccacct ggtgggctcc gggacgatgg aagacgtatc cgacgtaacc
      721 gtggctgtcc agaacgtcgg gcatggtcgc cgggtgtccg aaggagtcca cgttgtagcc
      781 tactttcggt ctcaccccga aacggtcctt gaaatagcgc tggccgtgga tgatctgacg
      841 gtgcaggccc attaccgtgg agaggttgac gtcgggctgg atgaactgcc cgcccgtgat
      901 gtgccactgg ccgctctcga cctgccgccg cacccgctcg aagagcccgg gatctatccg
      961 ctccacctgc tcgaagagcc aggactcccc gcgcgtgaat acgaactcgg ggtactcgtc
     1021 gcagcggtcc gcggcggaac ggaacgtggc cagcgcctcg tccaccccgg cctgccagga
     1081 ccacagccac acagggtcga tgtgggcgtt tcccatcatg tggactatca cgaaaaaacc
     1141 aaccccctcg gtttgtatga gcttgctatt cctaaccgac ggacagagcc ggctcgcttc
     1201 ccggactagc ctttgatagc tccggaggtc agggcgcgga cgatgaaccg ctgcagaatg
     1261 acgaaggtag cgatggcagg cagcatggcc agtatccctc ccgcggcgac cacttgttgt
     1321 gaggtctggg cgtagttgcc ctggaagttc aggataccga tggagatcgg gagcagcgag
     1381 ggcttgctga ggagtatgta gggtatggtg aactctcccc aggcgaggat cgccgtgagc
     1441 acgaacgccg aggtgaggcc tggcaggata agcggcaggg taaccctccg gaatgcgccg
     1501 aaacgcgtgc agccgtctac catcgccgcc tcgtccagag ctggagggat gccgtcgaag
     1561 aagcctttca gcatccaggt tgcaatcggc accaggatgg cgatgtagac catcgccgtg
     1621 ctgacgtgcg agtcgagtat gttcaggcga gcgaagtatc tatacagcgg gatgccgatc
     1681 accagcggag atatcatctg gagggcgagc accccaatca gcaggccgtt gttgccgcgg
     1741 aaggcaagac gggagaaggc gtacgccgcc ggggcggcga ccagcatcgc gccgatgccc
     1801 cccgccacgg agagcttgag agagttgatc aggaacaccg agaactgcgc cgtgtaaagc
     1861 acctcgacgt agttgtcgag cgtcgggttc tcggggatca gcctgagcga ggtgatgtag
     1921 acctcctgtg gggtccttat cgagagcgac agcacccaca gcagcggtcc gccgaagaac
     1981 accagggtgg cggcgaacac caggtaggtc agcacgtcgc cgatcctgcc tctggccttg
     2041 cgactcctca taccacaccc tccgaacgca gcaacctcct gtaaccgagc gtgatgacca
     2101 ggctgatgag gagcatgatc acggcgagca cggccccgcc ggccaggctg tactccctga
     2161 agatgacgtt gtacgtgtgg agggcgagca cctctgtcgc ctgtcctggg ccaccacccg
     2221 tgagcgctaa taccatgtcg aagctgttga gggtttgaat cgttatgagg atgatattga
     2281 tcaagagcat cggcctgagc tgcggtatgg tgatgtacca gaatgactgc agcgtcgatg
     2341 cgccgtccac ctccgcggcc tcgtagagct cgtcggggat cgactgcagg ccggcgtact
     2401 gcaggatcat gctgaaggcc gtccccctcc agacgttggc gattacgatc gacatcaggg
     2461 cgaggttggg gtcggagagc cacgccatgc cgtccagccc gacgggggcg agcaccagat
     2521 tggcaagccc gtagctcgcc tcgttgaata tgatggccca cacgatcccg atgaccacgc
     2581 ctggcatgac ccaggcggag agaattaccg accgcatgaa caccatgcct ggaaggcgcc
     2641 gcctcctggc cctgttgacg gcgaccgcga tcagcagtcc gagggcaagc tgcaagacga
     2701 tgctcgccag gacgaagatg gccgttatcc gtagcacctt gtacaggacc ggatcgctca
     2761 ggacgttctt gaacgtgtcc agggtgtatt cgtaatccgg cttcagcaag gaagcgtcag
     2821 tgaggctgaa ccgcaggacc tcgaacgccg ggtacaggta gaacagcgtg acgaccgcga
     2881 gcagcggggc catccagagc gttggcctgt tctcgcctat gagctcccga ggagcgaaag
     2941 ccttcttcgt catgatttct accgctactt cttctgctcc tcgtacgccg agagcgaagc
     3001 cttgtacgcc gtgtcgaggg cctcctcagg cgtcgcctcc tccgtgagga ccgtgccgat
     3061 ggcgacctga agttcctcgg acatcgtcgg gtagaccggg acccccggcc ttgccttgcc
     3121 gtgctggagc atggtgcgga agtcgtcgta gatcggctcg tcgaacttgt ctatctcgtc
     3181 gaagagtttg ggagaggtcg gcaactggcc ggttacctcg ttcatccccc ctacgaaaac
     3241 atccttggtg aactccatgc acatctcgac cttcttgggg tcgtcggaga aggagcctat
     3301 ggtccacccg ccgcttcccg acgacggcgt gtcgctggcg ctcatcgtcg ggaggagcga
     3361 cacctcccac ttcttgaact cttcgggcgg caaggtgtcc ttcaaggttg gatactggaa
     3421 gtcgccgccc tggaacatcg cgacctgctg cgcctgggcc gccgcctcga actctgcgta
     3481 gtccttgatc gtcgcgaccc gctggggcga ggcgccgctg tccacggtct ccttgaggaa
     3541 gttgaattcg ttcagcatgt acgtgcggtt cggctcctcg ccgaagatcg gcttgccctg
     3601 ctcgtcgacc agttcgccgc cctgactcca gaagtgggcc aggttatcga aggtggtgcc
     3661 ctcccacctt ccgccgttga agaggaagcc gtcgaccttc gagtccttct cctctgccgc
     3721 attcgcggcc tctattagct cgtcccaggt cctgggcgcc tccggtacca ggtccgtcct
     3781 gcggtacaga atccgcccgt cagtggcgaa ccagtaggcg tacatcttcc cgtcatcacc
     3841 gactacgatg tcgcggacga aggggaacag
//
LOCUS       DATAIR010000633         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_136297, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000633 DATAIR010000000
VERSION     DATAIR010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136297"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VHM69_15040"
     CDS             <1..575
                     /locus_tag="VHM69_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2741756.1"
                     /translation="RIPFDDAALRESWGVEALDRGLTGQDALREMLLRPTANIAGIQS
                     GYTGPGPKTIVPSEAFVKMDFRLVSGQSPGPVLKLIRSHLKERGFDDIEVVDLHGVEP
                     AKTPVDSPVVRIAVETWKDLGSEAIVYPTIGGSGPTSLIAAELGIPTIMTGNVANSGS
                     RIHSPNESITLEDYFDAIGYFARFFERFTS"
     gene            572..1156
                     /locus_tag="VHM69_15045"
     CDS             572..1156
                     /locus_tag="VHM69_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741757.1"
                     /translation="MTEEAGKGLDLPERVRTSLAAAVAQRGVQARVISTQGRESMRPP
                     QGHIIVAAREEEVEAVRGPARIVRRLEFYKTFYGPVVRLAMSVYPMEGEPLSAGTILN
                     VSQVSGDAALTGLGRQKSIYIHFYSAEGEDLTYAFSKEIPNAPEQRGETKKVLKMARD
                     AFTQTPEERRSFRAAVGLAERQFELPVPEPDEEG"
     gene            1190..2332
                     /locus_tag="VHM69_15050"
     CDS             1190..2332
                     /locus_tag="VHM69_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741758.1"
                     /translation="MQAGSASKDFVAKISQALEGAGRRGIHVTAGAGSCDLEAQQFAN
                     VPPEDEEQRPTGRPHHLEEPPGPRDLGGHDGTSRLCYFLDGVQSSREIGRIEMVPVIV
                     STVAAAIVNRCDRRFSRMPFESPPVVVQAVILPRSAGDAKVEAFWDLLLDAGFSELDP
                     DEVPSSPHLVLDSAEYVADADPSDYVGMRERARVRVRALRERLEGGMLRQWEGDDRTL
                     EDPDAWIAVDGQLENIRESNRRAIGLIKSVARPEFVGKDVGMLLDLEPGMRTTSFIPD
                     WQLRRATTDQRTSWYLRMWPPQRGADALGSLMRVEAPRDTEPDQVDEISRWILAERAP
                     LAKPDPRWPAMIYPIQYVEKILKPLVQGSERAYARLERQLASNGRN"
BASE COUNT          473 a          761 c          769 g          377 t
ORIGIN      
        1 gccgcatccc cttcgacgac gccgcgctgc gggagtcctg gggcgtcgag gcgctggaca
       61 ggggacttac cggccaggac gccctacgcg agatgttgct caggccgacg gcgaacatcg
      121 ccggcattca gtctggctac accggccctg gtcccaagac catcgtgcct tcggaggcgt
      181 tcgtgaagat ggacttcagg ctagtgtcgg gccagagccc cggtcctgtg ctgaagttga
      241 tccggtccca cctcaaagag cgcggcttcg atgacatcga ggtcgtcgac ctgcacggcg
      301 tcgaaccggc gaagaccccg gtggactcgc ctgtcgtccg tatcgcggta gagacgtgga
      361 aggacctggg cagcgaagcc atcgtttacc cgacgatcgg tgggagcggg ccgacatccc
      421 tcatagccgc cgaactcggg atacccacca tcatgacggg caacgtggcg aactcgggga
      481 gcaggatcca ctcgcccaac gagtcgatca cactcgaaga ctacttcgac gccataggct
      541 actttgcccg attcttcgag aggttcacat cttgacggag gaagccggca aggggctcga
      601 ccttcccgaa cgtgtcagaa ctagcctcgc cgctgccgtc gcacagaggg gcgtgcaggc
      661 gcgtgtgatc tctacgcagg gccgcgaatc gatgcgtccc ccgcaaggac acatcatcgt
      721 cgcagccaga gaggaagagg tagaggccgt ccgcggcccc gcccgcatcg tgcgccgcct
      781 cgagttctac aagacgttct acgggcccgt cgtccgtctc gcgatgagcg tatatccgat
      841 ggaaggcgaa ccgctctccg cgggcaccat cctcaacgtc tcccaggtca gcggcgacgc
      901 ggccctcaca gggctcgggc gccagaagag catctacata cacttctact ctgccgaagg
      961 cgaagacctg acctacgcgt tctccaagga gatccccaac gcccccgagc aacgcggcga
     1021 gacgaagaag gtcctcaaga tggcccgaga cgccttcacc cagacgcccg aggagcgccg
     1081 gagcttcagg gcagccgtcg gactcgccga gcggcagttc gagctacctg tacctgaacc
     1141 tgacgaagaa ggatagtctt cgaaggcgag cccgcgtata ctgccacgta tgcaagcggg
     1201 gagcgcttcc aaagacttcg tagcgaaaat aagccaggcc ctcgaagggg ctggccgcag
     1261 gggcatccac gtaaccgccg gagcaggttc atgcgatctt gaggcccaac agttcgctaa
     1321 tgttccccct gaggatgaag agcagaggcc gaccgggcgc ccgcaccatc tggaggagcc
     1381 gccgggacca cgggacctgg gcggccacga cggcacctcg cggctctgct acttcctcga
     1441 tggcgtgcag agctcgcgtg agatcgggcg catagagatg gtgccagtga tcgtgtcgac
     1501 ggtggcggcg gcgatcgtca accgctgcga caggcggttc tcgcggatgc cgttcgagag
     1561 cccgcccgtc gtcgtgcagg ccgtgatcct accccgcagc gccggtgacg cgaaagtcga
     1621 agccttctgg gaccttctgc tagacgcagg gttctccgaa ctcgaccctg acgaagtccc
     1681 ctcttcccca cacctcgtcc tcgactccgc tgagtacgtg gccgacgcag acccttcgga
     1741 ttacgtcggg atgagggaga gagcccgcgt gagggtcagg gcgctcaggg agcggctgga
     1801 gggcgggatg ctcaggcagt gggaagggga tgaccggacg ctggaggacc ccgacgcctg
     1861 gatcgcggtg gacgggcaac tcgagaacat ccgtgagtcg aaccggcgcg ccatcggcct
     1921 cataaagagc gtcgctcgcc ccgagtttgt gggcaaggac gtcggaatgc tgctcgacct
     1981 ggaaccaggg atgcggacga cgagcttcat cccggactgg cagttgagac gtgccaccac
     2041 cgaccagcgt acctcctggt acctgaggat gtggccgccg caacgcggcg cggatgctct
     2101 aggttcgctc atgcgcgtcg aagcgccgcg tgatactgaa ccggatcagg tcgatgagat
     2161 atcgcgttgg atcctcgccg agcgggcacc cctggcgaaa ccagacccga ggtggccagc
     2221 catgatctac ccgatccaat acgtcgagaa gatcctcaaa cctctggtgc agggttcgga
     2281 gagagcctac gcacggctgg agcgccaact ggcatcgaac gggaggaact agatgctgca
     2341 cgaagatttc ctggacctga tctcaggccc catcggcagg
//
LOCUS       DATAIR010000634         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_320683, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000634 DATAIR010000000
VERSION     DATAIR010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320683"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1056)
                     /gene="era"
                     /locus_tag="VHM69_15055"
     CDS             complement(136..1056)
                     /gene="era"
                     /locus_tag="VHM69_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX2741759.1"
                     /translation="MTEATGVSEKNGLRSGFIAVVGRPNVGKSTLVNKLVGRKVSITS
                     SRPQTTRSPIRGVRNGPHYQAIFVDTPGSQKPRDTLRARMQEQVLDSLSESDVILLLF
                     DAQQAASGLGNGDKYVARLVAESGTPAIASINKTDLLRSREEALPIVQAVSDLGDWDE
                     VFQISAANGTNVEPLMESVVDLLPPGPRYFPEGTVTDYPESLVLAEYVREKALNVLRE
                     EVPHAVAVEIEDVERKENVTVVYAVIHVERATQRMIVLGKGGRTIKKIGTEARRDVER
                     LLGTRIYLDLKVKVSPGWRSDKMFLERLGL"
     gene            complement(1053..1442)
                     /locus_tag="VHM69_15060"
     CDS             complement(1053..1442)
                     /locus_tag="VHM69_15060"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX2741760.1"
                     /translation="MDLETVMEAARAASSRAYAPYSGFQVGAAIVTSGGAIHAGSNVE
                     NASYGLSICAERNAATTMAHADPEDRHIELVAVYSPNTSPCFPCGACRQFLREFGCKE
                     VVVEDGSGLRRYPFEEMLPNSFGPEDL"
     gene            complement(1454..2200)
                     /locus_tag="VHM69_15065"
     CDS             complement(1454..2200)
                     /locus_tag="VHM69_15065"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX2741761.1"
                     /translation="MAKQVKQPGNKGQQGVARSFEHAYKGMIFAVRTQRNMRFHVVVA
                     VAVLVASLLVGVSKLELAVLVLTILLVFVTELFNTAMEFVVDLATKEYHPLAKLAKDV
                     SAGAVLVSSVGALLVGYLVLADDLGPLSLATLETIRRQPAHLTLVSLGVVVLVVLLGK
                     ALTHSPNTFAGGMPSGHAAVAFAGWVAASFIAVAGGAGSYAGLVSVISLLMALLVCQS
                     RVESGLHSVYQVVMGAVIGALVTTAAFQLL"
     gene            complement(2184..2594)
                     /gene="ybeY"
                     /locus_tag="VHM69_15070"
     CDS             complement(2184..2594)
                     /gene="ybeY"
                     /locus_tag="VHM69_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2741762.1"
                     /translation="MSVAVLDEVGHAGLDEARASRIFAATFSTRGLDLSRIGEVSVAL
                     VDSETVRDLNLRFRGKDEPTDVLSFEIDGPYGEMVGEVVICPRCASLEMGVEELVVHG
                     ALHLSGMDHGEDFEASEMAKVQRAVMEELRGEAG"
BASE COUNT          528 a          907 c          841 g          537 t
ORIGIN      
        1 gtccttgcag aggcgtacga tctcctgctc agtggcctcg ggcttcaaga gcgtatggtc
       61 caccgtcttc gcgaactcgc gcaccctcat cgccaactct accctcttct cactcgacac
      121 tacgacgcac ggtgattata aacctaaccg ctcgagaaac atcttgtcac tcctccatcc
      181 cgggctaacc tttaccttca ggtcgaggta aatacgcgtg ccgagcaggc gttcgacgtc
      241 gcggcgggcc tcggtcccga tctttttgat ggtcctgccg cctttgccga gcacgatcat
      301 gcgctgtgtc gcccgctcga cgtggatgac ggcgtaaacg acggtaacgt tctccttgcg
      361 ttctacgtct tcgatctcga cggccaccgc atgagggacc tcttcgcgca ggacgttaag
      421 cgccttctcc cgcacgtact cggcgaggac cagcgactcg gggtagtcag tgacggttcc
      481 ttcaggaaaa tagcgaggtc cggggggcag aaggtcgacg acagattcca tcaacggctc
      541 gacgttcgtc ccgttcgcag cgctgatctg gaacacctcg tcccaatccc cgagatcgga
      601 gaccgcctgg actatcggca gtgcctcctc gcgagagcgg agcaggtcgg tcttattgat
      661 agaagcgatc gccggagtgc ctgactcggc gacgaggcgg gcgacgtact tgtcgccgtt
      721 tcccagtcca gatgcggcct gttgcgcgtc gaagagcaag agtatgacgt ccgactcgga
      781 gaggctgtcc aggacctgct cctgcatcct ggcgcgcagg gtgtcgcggg gcttctggga
      841 gcccggggtg tcgacgaaga tcgcctggta atgcgggccg ttgcggacgc cccggatcgg
      901 gctccgcgtc gtctgaggac gggaggaggt gatcgaaacc ttccttccca cgagcttgtt
      961 gaccagtgtg ctcttcccga cgttgggccg tcccacgaca gcgatgaaac cgcttcgtag
     1021 cccgtttttc tcagagacgc cggttgcttc tgtcacaggt cctccggacc gaaagagttg
     1081 ggaagcattt cttcgaatgg gtagcgacgc agccctgagc cgtcctcgac gacgacctcc
     1141 ttacaaccga actcgcgcaa gaactgcctg caggcaccgc aggggaagca cggcgaggtg
     1201 ttggggctgt acacggcgac cagctctatg tgcctgtctt ccgggtcggc gtgagccatc
     1261 gtcgtggcgg cgttgcgctc cgcgcagatc gacagtccgt aggaggcgtt ctcgacgttc
     1321 gagccggcgt gaatagctcc accggacgtt acgatggccg cgccgacctg gaatccgctg
     1381 tacggcgcgt aagcccggct cgaggctgcg cgagccgcct ccatcacagt ttccagatcc
     1441 aacttctctc ccctcatagc agttgaaaag cggctgtggt gacgagggcc ccgatcaccg
     1501 cccccataac cacctggtag acgctgtgaa ggccgctttc gacgcggctc tgacacacga
     1561 gcagggccat gaggagcgag atcacggaga ccagaccggc ataggatccg gcgccacccg
     1621 cgacggctat gaagctcgcc gcgacccacc ctgcgaaggc gaccgcagcg tgtcctgaag
     1681 gcatccctcc cgcgaacgtg ttcggtgaat gcgtcagggc cttgcctaag aggactacca
     1741 gcacgacgac accgagcgag acgagcgtaa ggtgggccgg ctggcgccgt atggtctcca
     1801 gggtagccag agagagcggg ccaagatcgt cagcgaggac cagatagccc acgagcaggg
     1861 ctcccacgct cgagacgagt actgccccgg ccgagacgtc tttggcgagc ttggcgagcg
     1921 ggtggtattc cttcgtagca aggtcaacga cgaactccat ggccgtgttg aacagctcgg
     1981 tcacgaagac caggaggatg gtaaggacaa gtacggcgag ctccagtttg cttacgccaa
     2041 caagcaggct cgccaccagg acagcgacag ccacaacgac gtggaagcgc atgttgcgct
     2101 gggtgcggac cgcgaagatc atacccttgt acgcgtgctc gaagctgcgg gccacaccct
     2161 gctgtccttt attgcccggc tgcttaacct gcttcgccac gcagctcctc catcacggcc
     2221 ctctggacct tcgccatctc gctcgcctcg aagtcctccc cgtggtccat gccgctcaag
     2281 tgtagcgctc cgtgcaccac gagttcctcg acccccatct caaggcttgc gcaacggggg
     2341 cagatcacga cctcgccaac catctctccg tatggtccgt cgatctcgaa gctgagcacg
     2401 tcggtcggct cgtctttacc tctgaacctc aagttgaggt cgcggaccgt ctctgaatcc
     2461 acgagggcaa ccgaaacctc gccgatacga ctgaggtcga gaccccgtgt ggaaaaggta
     2521 gcggcgaaga tgcgggaggc acgcgcttcg tcgagtccgg cgtggcccac ctcgtccaga
     2581 acagcgacgg acatcggcgg atagctcctc aggtcgtatc tctctctacg gtatccactg
     2641 actacttcgc cgccttcccg ttgcgggtat cgccgtgagg ttcagggtac tcgatgcgcg
     2701 gcccgagaaa acctataagc gcctccctta tagcctcccc cgcctcatgg atatcgtgca
     2761 tggtcagctg gcattcgtcg aactggccgt cctgaagctt cctcctgacg gta
//
LOCUS       DATAIR010000635         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_320805, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000635 DATAIR010000000
VERSION     DATAIR010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320805"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..834)
                     /locus_tag="VHM69_15075"
     CDS             complement(205..834)
                     /locus_tag="VHM69_15075"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX2741763.1"
                     /translation="MSLGAISDAGARGLLDPDSPVRRHYEDLANNLLRLGAGSVVVTS
                     PEPGAGRTSVCLGLGAALAGMGRRAAIVDCNLENPQLHRILGEPNFVGLTSGLEGSKP
                     LEHYGHEVIPGLLVVPTGPVLLDPAYRLEDERVGDVVRGLQKSRDLVLLDAPVAGSVL
                     RSPNLSGGFDGVLLVVHASKTPKSVARGTTDDLLEAGVNLLGVVLNGCS"
     gene            complement(836..1558)
                     /locus_tag="VHM69_15080"
     CDS             complement(836..1558)
                     /locus_tag="VHM69_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX2741764.1"
                     /translation="MTFTSGWRGDSRDEPSLSEVLYILQRRRLLVVGVVLVLAGVALL
                     VGLFRQPVYTAEAKVSVTPQETLDSEEARLAFLEEVRGDVVTQEMLSEVRRRAGWEAE
                     PEAFSARLDPRTAVTRDGRTGLQILFSGTEPGQAARAANAYAELFVEGVENLGDEPLG
                     DGIPAAEASIEQRAVPGGYSPRPLIYAAIAAVAGLLLGGTVALLLEGRASGWRGVRDA
                     ELTLRAPVLGAIPDYSSKGTEG"
     gene            complement(1545..2342)
                     /locus_tag="VHM69_15085"
     CDS             complement(1545..2342)
                     /locus_tag="VHM69_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2741765.1"
                     /translation="MIFRISAGFLVGVLALLATVLYLSDYYIVEQQRLAAAGDVQGAL
                     EASRKAVRLDPFDTDALEAQSFLLQQQQKYDESAQALRGAIERDPNNYLPHLMLANLE
                     LATANYDAAAESYRDVLRLNPNASLASNSLAQALVRQGKLEEAARVYEKLKNDRTIDY
                     QGLYNLGRIQVRRGEPGDGLKNLKRARNMAAADLDEMRGPVKKQRQQLLVSMDLDIAD
                     ALVVQGRYGQAREILTQSPSEQAQGLLGLLDSDPDAYREQVINSDIY"
BASE COUNT          465 a          894 c          748 g          460 t
ORIGIN      
        1 tcgtcgtaga ggccgtctcg gcgtatcccg taagttgccg ctcccaccag agcaagcagt
       61 acgaggccct tgaagaaggc aagcagacgg ccaggacggt gctgctatcc tccgttctcc
      121 tcgccgccgg cgccgaagaa gcgtcctctg gcgtcgcctg aggattggac ctctttcacc
      181 cctctacccc accacgagcg ggcgtcacga acatccgttc aacaccaccc cgagcaggtt
      241 gacccccgcc tccagcaggt cgtcagtcgt cccgcgtgcg acgctcttgg gggtctttga
      301 ggcgtggacg acgagcaaga ccccgtcaaa gcctcccgaa aggttcggcg agcgtagcac
      361 gctccctgcc accggcgcgt cgagcagtac cagatcccgg ctcttctgga gaccgcgcac
      421 gacgtctccg actctctcgt cctcgagacg gtaggcgggg tccaacagaa cgggaccggt
      481 cgggacgacg agcagcccag ggatgacctc atgcccgtag tgctcgaggg gcttgctacc
      541 ctccagaccg cttgtaagcc ccacgaagtt cggctcacct agtatcctgt gcaactgtgg
      601 gttctcgagg ttgcagtcca cgatggcggc ccgcctcccc atgccggcca gggcggcacc
      661 gaggccgagg cagacgctcg tgcgaccggc tcccggctcg ggactggtta cgacgacgct
      721 gccagcccca agcctgagaa ggttgttcgc caggtcttcg taatgccgcc tcacgggaga
      781 gtcggggtcg agtaagcccc tggcgcccgc gtcgcttatc gccccgaggc tcacgtcaac
      841 cctcggtacc cttcgaggag tagtcaggta tggccccaag caccggcgcc ctcagcgtca
      901 attcggcgtc acgcacaccc cgccagccac tggcccttcc ctcgagtagc aatgccacgg
      961 tgcccccgag cagcaacccg gccactgcgg cgatggcggc gtagatgagg ggccgcggac
     1021 tgtatcctcc aggcacggcc ctctgctcta tgctggcctc cgccgccggt atgccgtcgc
     1081 cgagcggctc gtcgccaagg ttctccactc cctcgacgaa caactcggcg taagcgttcg
     1141 ccgcgcgggc cgcctgccct ggctcggtgc ctgagaacag tatctgcaac ccggtcctgc
     1201 cgtccctcgt cacggcggtc ctcggatcta gcctcgctga aaaggcctcc ggctccgcct
     1261 cccagcctgc cctgcgcctt acttcgctca acatctcctg cgtaaccacg tcgccacgca
     1321 cctcttcgag gaatgcaagc ctcgcttcct cgctgtcgag tgtctcctgg ggcgtgacgc
     1381 tcaccttcgc ctcggctgtg tagacaggct ggcggaagag cccgacgagg agggcgaccc
     1441 cagcgaggac gagcacgacg ccgacgacga gcaaacgcct tctctgcaag atatacagga
     1501 cctcggagag cgaaggttcg tcccgcgagt ccccgcgcca gccgctagta aatgtcacta
     1561 tttatcacct gttcgcggta cgcgtctggg tccgagtcca acagaccaag gagtccctgc
     1621 gcctgttcgg agggactctg ggtgaggatt tcgcgcgcct gcccgtacct gccctggacg
     1681 acgagagcgt cggcgatgtc gaggtccatg gatacaagga gctgctgccg ctgcttcttt
     1741 acgggtcccc tcatctcgtc caggtcggcc gccgccatgt tcctggcacg ctttaggttc
     1801 ttcaggccat caccaggctc gcctcttcgg acctggatcc tcccgagatt gtaaagtccc
     1861 tggtagtcga tcgtcctgtc gttctttagc ttctcgtata ctctcgccgc ttcctccaac
     1921 tttccctgcc tcacaagcgc ctgggcgaga gagttgctcg ccaggctggc gttcggattg
     1981 agcctgagca cgtcacggta gctctccgcc gcagcatcgt agttcgccgt ggcgagctcg
     2041 aggttggcca acatcaggtg gggaaggtag ttgttgggat ctcgctctat cgcgcccctg
     2101 agagcctgcg cggactcatc gtacttctgc tgctgctgca gtaggaatga ctgcgcctcg
     2161 agagcgtcgg tgtcgaaggg gtccaggcgc accgccttcc gcgaagcctc cagcgccccc
     2221 tgaacgtccc cggcagcagc gagacgctgc tgctcgacta tatagtaatc agacaggtag
     2281 agtaccgtag ccagcagggc caataccccc accagaaacc ccgcggagat tctgaagatc
     2341 acactgctca aaaggatcac cgcgcatgag gatatccaca accctccgca aatacaagaa
     2401 agatacggcc gccgagctat gaagacacaa agaagcgggc ccccgaagga gcccgcctca
     2461 tgatgcctaa cgtgtccggt tacttaccga acgatcctgc gtgccagaag tccaccggca
     2521 acaagcaggg cgccagcacc gagcgcgagc agcgaagcgc cacccgt
//
LOCUS       DATAIR010000636         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_320847, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000636 DATAIR010000000
VERSION     DATAIR010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320847"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1271)
                     /gene="obgE"
                     /locus_tag="VHM69_15090"
     CDS             complement(33..1271)
                     /gene="obgE"
                     /locus_tag="VHM69_15090"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX2741766.1"
                     /translation="MQFVDEASFVVRGGRGGDGSVSFNREKYKPRGGPDGGRGGDGGA
                     VVLHATEDLSTLEPYAYRNVVKAGRGGHGSGNNRAGERGKEIVLDVPVGTQIFDDDGL
                     LADLSEPGETFVAAHGGEGGRGNGSFATSTRQAPAFRERGLPGEEREIRLELKVLSDV
                     GLVGLPNAGKSSLLRALSAARPRVGDYPFTTLSPQLGVVDERAYRGPFVVADIPGLIS
                     GASEGRGLGNRFLRHVARARLLALVLDASEDPEGAEGTLRAELHAARLSEKPSLVVLN
                     KVDLLDEELRAYLREAFPQASQVSAATGEGVAGLRDRFEEMLREMGEHAVTQPAEREH
                     RVFRPTWKGVRVEQENGGYVVSGEGVERLALKTDWGNMEGVERFQRELEKIGVVGALR
                     RAGAEPGDEVRIGETVFDFQ"
     gene            complement(1303..1578)
                     /gene="rpmA"
                     /locus_tag="VHM69_15095"
     CDS             complement(1303..1578)
                     /gene="rpmA"
                     /locus_tag="VHM69_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002088814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX2741767.1"
                     /translation="MAHKKGGGSSRNGRDSAGRRLGVKAYGGQVVTAGSIIVRQRGSK
                     VHAGQNVGKGSDDTLFARIDGRVNFDRSRGKSVVSVVEVPVLEEATA"
     gene            complement(1568..1867)
                     /gene="rplU"
                     /locus_tag="VHM69_15100"
     CDS             complement(1568..1867)
                     /gene="rplU"
                     /locus_tag="VHM69_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX2741768.1"
                     /translation="MFAVVKSGGKQYRVTEGDEIVVDRVKGEVGDSVELPVGFSVDDE
                     GFDLGEKTARVEILEHLRGDKIHVYKYKPKKDYRKKTGHRQELTLVKVLEVSDGS"
BASE COUNT          357 a          773 c          577 g          394 t
ORIGIN      
        1 tcccgccgaa gatccctatc ttcaagtcca cctcactgaa agtcgaacac cgtctcgcca
       61 atcctgacct cgtcgcccgg ctcggcccca gcccgccgca gggcaccaac aaccccgatc
      121 ttctcaagct cgcgctggaa gcgctcgacg ccctccatgt tgccccaatc tgtctttaga
      181 gcgagccgct cgaccccctc ccccgagacg acgtagcctc cattctcctg ctccaccctg
      241 acccccttcc acgtcggcct gaagaccctg tgctcgcgct cggccggctg cgtaacggcg
      301 tgctcgccca tctcgcgcaa catctcctcg aaccggtcgc gcagtccggc gaccccttct
      361 cccgtggcgg cggagacctg agacgcctgt gggaaggcct ctcgcaggta cgccctgagc
      421 tcctcgtcga gcaggtcgac cttgttgagg acgacgagag aaggcttctc cgagagcctg
      481 gcggcgtgaa gctccgcccg cagcgtcccc tccgcgccct cagggtcctc gctcgcgtcg
      541 agcacgagcg cgagtagccg cgcccgcgca acgtgcctga ggaagcggtt gccgagtccc
      601 ctgccctcgc tcgcgcccga gatcaatccg ggtatatccg ccacgacaaa gggtcctcgg
      661 tacgccctct cgtcaacgac cccgagctgg ggcgacaggg tggtaaaagg atagtcgccc
      721 accctcggac gggcggcgct cagggcgcgg agcagcgaag acttccccgc gttcggcagg
      781 ccgacgagcc cgacgtcgga gaggaccttg agctcgagcc ggatctcacg ctcctccccg
      841 ggcaatcctc tttccctgaa ggccggagcc tgacgcgtcg aggtggcgaa agaaccgttt
      901 ccgcgccccc cttctccacc gtgtgcggcg acgaaggtct cccccggctc ggagaggtcg
      961 gcgaggaggc cgtcatcgtc gaagatctgg gttccaacgg ggacgtcgag gacgatttcc
     1021 ttcccccgct cgcctgccct gttgtttcct gaaccgtgtc cgcctcggcc cgccttcacg
     1081 acgttgcgat aagcgtatgg ttccagggtg gagaggtctt cagtggcgtg caggacaacg
     1141 gcgccgccgt caccgccacg gccaccgtcg gggccgccgc gcggcttgta cttctcgcgg
     1201 ttgaaagaga cgctgccgtc gccaccgcgg cctccgcgga cgacgaagct ggcttcatct
     1261 acgaactgca aaggggctac agacccccgg aacgcgcgat cgtcaggccg tggcctcctc
     1321 gagaactggc acttcgacca cgctcaccac gctcttgccg cgcgagcggt cgaagttgac
     1381 ccttccgtct atccttgcaa agagcgtgtc gtcggagccc ttgccgacgt tctgaccagc
     1441 atgcaccttc gagccgcgct ggcgtacgat gatgctgccc gcagtgacca cctggccccc
     1501 gtatgccttc actccgagtc tcctacccgc cgaatcgcgg ccgttacgcg aggagcctcc
     1561 gcccttctta tgagccatct gatacctcca gcaccttgac cagggtcaac tcctgcctgt
     1621 gcccggtctt cttcctgtaa tccttcttcg gcttgtactt gtagacgtgg atcttgtccc
     1681 cccgcagatg ctcgaggatc tccaccctgg ccgtcttctc gccgaggtcg aacccctcgt
     1741 catctacgga gaagccgacc ggcaactcca cggagtcacc gacctctccc ttgacgcgat
     1801 cgacgactat ctcatcgccc tccgtcaccc tgtactgttt tccaccgctt ttcacaaccg
     1861 caaacatcac gcgctcctag gatttatcaa gctcagacga gcgtacaaaa cgtgctatac
     1921 ggggcaacag tatacccgcc atcctccgta tcgaccagca attgtgctcg cacgtaccct
     1981 cagtcgttgc cgaaagcgtc ccgattcggt atcttttggt catgcgtgca gtaaggacca
     2041 gtccagcggc tgtcgtctct cgtggcaaga agcccgagaa ggtgagcatc acacaccgcc
     2101 t
//
LOCUS       DATAIR010000637         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_458749, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000637 DATAIR010000000
VERSION     DATAIR010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458749"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VHM69_15105"
     CDS             <1..549
                     /locus_tag="VHM69_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2741769.1"
                     /translation="VAEVLGHPPDALALPVGNAGNITSYWKGFRELRESGRSDSLPRM
                     LGFQAEGASPLVAGHDFENPETVASAIRIGSPASKEGALSAARDSGGLIESVSDAQIL
                     AAQEMLAHEEGVFCEPASAAGIAGLLKLTGESRGPEGTVVSVLTGHGLKDPDVILGRV
                     QLPDPVPATFEAVAGQMGRAIA"
     gene            546..1472
                     /gene="thrB"
                     /locus_tag="VHM69_15110"
     CDS             546..1472
                     /gene="thrB"
                     /locus_tag="VHM69_15110"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2741770.1"
                     /translation="MISVRVPATSANLGPGYDAVGLALSLSMRIGLDRAPQPAIEVHG
                     TGADLIPTGPEHPAYRAARFVAELVGESDIHFSLVQENHIPPTRGLGGSAAALVGGAV
                     AANDLFGGQIAAPDLLNIVCELDGHPDNAAPALLGGLVIGTLTPSGVSAVRLEPKNLK
                     VVVAVPDFAVSTTAARQALPDYIPHRDATFNVGRSGLLLGALATGEYHLLRIAMQDRL
                     HQPYRSHLIPGLEDVIEAALASGAYGACLSGSGPTVLAFAPETSAPEIASDMRAAFEA
                     RGVRAESWALDVDLAGARVEPPEVVPTPSPVF"
     gene            complement(1501..2076)
                     /locus_tag="VHM69_15115"
     CDS             complement(1501..2076)
                     /locus_tag="VHM69_15115"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX2741771.1"
                     /translation="MSLTQPQPILPMVAGNWWALLLRGIAAVLFGLAALFWPGLTLYV
                     LVIFFGAYVLVDGVFAIAAGIRGSGGRGWLLLAEGVLGVLAGLVALFWPGISALVLLY
                     VIAAWAIFTGILKVVIAISLRREIENEWLMILSGVLSVVFGVVLAALPGVGLLSLVWL
                     VGIYALIFGVALIVLGFRVRGHRRENSGEVS"
     gene            complement(2283..3695)
                     /locus_tag="VHM69_15120"
     CDS             complement(2283..3695)
                     /locus_tag="VHM69_15120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2741772.1"
                     /translation="MNKFRDSGRLPILALLCAAQFMVVLDFSIINVALPSIRDDFGLS
                     RAGLQWVITGYVLTFGGFLLLGGRVADIYGRRLVFVAGLSLFVVASLAGGFASSAAVL
                     VGARAAQGLGAAAVSPAALSILTTSFREGAERNRALGIWGGVAAGGFSAGVILGGFLT
                     EILGWEWVMFVNVPIGGVVAILALFLLQESEGEAAGRKLDLPGAVSVTAGLVALVYAL
                     SGAGGAGFLSPRTVLSFGLALALLAGFWIIEWRTRDPLVPLGVFRRSTLTGANVVGLL
                     IIASAAPMIFLITLYMQRVLGYSAIEAGLAWLPHGLASIFASSVGGRLATRIGVRPTL
                     IGGLTVLAVSLGLLSRVSAGGVFWTDVLPGTLLVSFGIVLPLVAVSVAATSGVRDSEQ
                     GLASGLLSTMQQIGAALGLALLVAISATHTEALIGAGVAHPEALARGFRYALVFGLGF
                     PAMGLLIALLTMRGRRLQED"
BASE COUNT          781 a         1363 c         1195 g          574 t
ORIGIN      
        1 gtcgctgaag tcctcggaca tccccccgac gccctggccc tgccggtggg gaacgcgggc
       61 aacatcacct cgtactggaa agggttcagg gaattgagag aatcgggacg cagcgactca
      121 ctcccccgta tgctcggctt ccaggccgag ggtgcttcac ccctggtcgc aggccacgat
      181 ttcgagaacc ccgaaaccgt ggcgagcgcc atccggatag gttcgccggc gagtaaagaa
      241 ggggccctct cagccgcgcg cgattccggc ggcctcatag agagcgtatc ggacgcccag
      301 atcctcgccg cccaggagat gctcgcccac gaggaggggg tcttctgcga gcccgcatcg
      361 gcggcgggga tcgccggact cttgaaactc acaggcgaga gccgcgggcc tgaaggcacg
      421 gtcgtgagtg tcctcaccgg acacgggctc aaggaccctg acgtgatcct cggtcgtgtg
      481 cagctgccgg atcccgtccc cgcgaccttc gaggcggtcg ccggacagat ggggagggct
      541 atcgcgtgat ctccgtccgc gtcccggcca cctcggcgaa cctgggacca ggctacgacg
      601 cggttgggct cgcgctctct ttgagcatgc ggatcgggct ggacagggca ccgcagccag
      661 cgatcgaggt acacggtacc ggggccgacc tcatccctac cgggccggag catcctgcat
      721 acagggcggc gcggttcgtt gccgaactcg tcggcgaatc ggatatccac ttcagcctcg
      781 tgcaggagaa ccacataccg ccgacgcggg gcctgggcgg gagcgccgcc gcgctcgtcg
      841 gcggggccgt cgcggcgaac gatctgttcg ggggtcagat cgccgccccc gacctcctca
      901 atatcgtctg cgagctcgac ggacatccag acaacgcagc cccggccctc ctcggcgggc
      961 tcgtgatcgg cacactcacg ccgagcggcg tgagcgccgt gcgcctggaa ccgaagaacc
     1021 tcaaggtcgt cgtcgccgtc cccgacttcg cggtctcgac caccgcggcc cgccaggccc
     1081 tccccgacta catcccccac cgcgacgcca cttttaacgt cggacgctcc ggtcttctgc
     1141 taggggccct ggcgacaggc gagtaccatc ttctgcgtat cgccatgcag gacaggctcc
     1201 accaacccta ccgctcccac ctcatccccg gactcgaaga cgtcatagaa gccgcgcttg
     1261 ccagcggcgc ctacggcgcc tgcctctccg gctcgggccc caccgtcctc gccttcgcgc
     1321 ccgagacaag tgcccccgag atagcgtcag atatgcgggc ggctttcgag gctaggggag
     1381 tgcgcgccga gtcgtgggcg ctcgacgtcg acctcgcagg ggcccgcgtc gagccgccgg
     1441 aagtcgtccc gacaccgagc cccgtgttct agggcctact gctcggaccg tggagccagg
     1501 ctagctcacc tctccgctgt tctcgcggcg atggccccgc accctgaagc ccaggacgat
     1561 cagcgccacg ccgaagatca gcgcgtagat gccgaccagc cacaccaacg agagcagccc
     1621 aacgccaggc aatgcagcca ggaccacccc gaagactaca gagagcacac cgctcaggat
     1681 catcagccac tcgttctcta tctcccgcct gagcgagatg gcaatgacca ccttcagtat
     1741 gcccgtgaag atcgcccacg ccgcgatcac atacagcaac accagggccg atatgccggg
     1801 ccagaacaga gcgacgaggc ccgccagcac tccgagcacg ccctcggcca gaagcagcca
     1861 cccgcgccct cccgatcccc taatgcctgc ggctatggcg aagacgccgt ctaccaggac
     1921 gtaggcaccg aagaagatca cgaggacata cagggtcagc ccgggccaga atagcgcggc
     1981 cagcccgaag agcacggccg ctatccctct gagtagtagg gcccaccagt tgccggctac
     2041 catcggcaga atgggctgtg gttgcgtaag gctcatgttc gcatctccct tatcccccga
     2101 ctcctgcaca atacttgctc cgccccaact ctaatctgcg caagggttct cgtactgaac
     2161 agaatggccc aattggctga tcagaattga ctaataacgc cccgaagaag gtcaaagtag
     2221 ggctaaagaa cctggtgcgg tcgatcctcg tgctgccact ctcgttctag cgtggctcct
     2281 gctcagtcct cctgaagccg tctcccccgc atggtgagaa gggcgatcag caatcccatg
     2341 gcagggaacc cgaggccgaa gacgagcgcg tagcggaacc cgcgggcgag tgcctcgggg
     2401 tgtgcgacgc cggctccgat cagggcctcc gtgtgggtgg cggagatggc gacgagcaga
     2461 gccagcccga gcgcggcgcc tatctgctgc atcgtgctga gtagaccgga ggcaagaccc
     2521 tgctccgagt ctctcactcc tgaggtggca gcgacggaga cagccacgag aggcaggaca
     2581 atgccgaaag agacgagtag cgtgcctggc aagacatccg tccagaaaac tccgccggcc
     2641 gagacgcggc taagcaggcc gaggcttacc gcgagcacgg tcagaccccc gatcagggtc
     2701 ggccttacgc cgatacgggt ggccagccgt cccccgacgc tggacgcgaa gatgctcgcc
     2761 aggccgtgag gcagccaggc gaggccggcc tctatcgccg agtagccgag taccctctgc
     2821 atgtagagtg tgatgaggaa gatcatgggc gccgctgagg ctatgatcag gagccctacc
     2881 acgttcgcgc ccgtgagcgt gctgcgccgg aagacgccga gcggtacgag cgggtcccgc
     2941 gtccgccact ctatgatcca gaacccggcg agcagcgcga gcgcgaggcc gaaggagagc
     3001 actgtacgcg gcgagagaaa gccggcacca ccagccccag agagcgcgta gacgagcgcg
     3061 acgaggccgg ccgtgacgga gacggcaccg gggagatcca gcttcctccc ggccgcctct
     3121 ccctcgctct cctgcaggag gaagagcgcg aggatcgcca ccacgccgcc tatgggcacg
     3181 ttgacgaaca tcacccactc ccaacccagg atctcggtca ggaagccacc aaggatgacg
     3241 ccggctgaga acccccccgc cgcgaccccg ccccagatgc ccaaagcccg gttgcgctcc
     3301 gcgccttccc ggaagctcgt cgtgagtatg gagagcgccg caggtgagac ggccgccgcc
     3361 ccgagaccct gcgcggcgcg cgcccccacc aggacggccg cggaagaggc aaatcctcct
     3421 gccagagaag ccaccacgaa tagcgagaga ccggccacga agaccagccg gcgcccatag
     3481 atgtctgcca cccgcccacc gagaagcagg aatcccccga acgtcaggac gtaccccgtt
     3541 atcacccact ggagccctgc ccgtgagagg ccgaagtcgt cacggatgga aggcagggcg
     3601 acgtttatga tcgagaagtc aagcaccacc atgaactgcg cggcacataa caacgccagg
     3661 ataggtaaac ggccagagtc cctgaacttg ttcaacaaac ctcccgattt gctacatact
     3721 tcttacaacc tgtttctttt tgccctgagc ctgcgagact aaacgggttc aaaccttgac
     3781 aaccctctac cgttgtgtaa cccttcacca cacttgcggg ctaaacgagc gagggctttt
     3841 gacggaagtc tcgaaaacgg cgcagaagaa ggtctcgggt ttgatgctca agatctcacg
     3901 cctgctcgct ccc
//
LOCUS       DATAIR010000638         9378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_136685, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000638 DATAIR010000000
VERSION     DATAIR010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136685"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..282)
                     /locus_tag="VHM69_15125"
     CDS             complement(112..282)
                     /locus_tag="VHM69_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741773.1"
                     /translation="MANVSIAYGTGAGQTAKISAYIVRDRCHDAELIDEFQRFTEELL
                     RVLTRETMLEHG"
     gene            complement(325..1302)
                     /locus_tag="VHM69_15130"
     CDS             complement(325..1302)
                     /locus_tag="VHM69_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2741774.1"
                     /translation="MDSQHGGVIRPDRVLLATDGSKDAMLAARVATDLSRKTGSELHV
                     AHAWHYHIQGLGYPAVVWTDYSHIYEKEARRLLGAQVDSIEATGDLVAEPHLLHGPPI
                     DAILDLCEDLRPGLLVMGSRGLGPVGRIFVGSVSEGVVHHARCPVLVVRGGDESWPPD
                     RIITGDDGSESAGLAAEMAAGIGSMFGAEDVLVRAHRNPPEPIGGWSAEDRRRLEKQR
                     SREAEHLNRRAEALGEAVGSRPETRVVDADATLALLLVAEEGDERKTLLSVGSRGLGV
                     IGRVRLGSVSTNVLRAAQGPVLIYSKPEAEITASREATVVVSRSGPATL"
     gene            complement(1339..2049)
                     /locus_tag="VHM69_15135"
     CDS             complement(1339..2049)
                     /locus_tag="VHM69_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2741775.1"
                     /translation="MTFALSTGQEAHDKIAVAELPETLESLTGALTADAVFIVDPEFR
                     IVHWDARAESLTGLLAEEMVGGFCYEVLTGESEDGASFCAHVYSAVRLAWAGQPAPGY
                     EVRVFTRSGRERWVGVSSLAVETGEGLYLVHMVRDTQAAHETLEMARQVLRLSRQDAP
                     EECTVRNHRDTPVLTPRQLEVLGLLAAGKSAREICRELYLSQATVRNHIRALLLALGA
                     HSQLEALARAREAGLLDG"
     gene            2363..5182
                     /locus_tag="VHM69_15140"
     CDS             2363..5182
                     /locus_tag="VHM69_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741776.1"
                     /translation="MVTRKTNEGREPGLESERVAHDQMANILESISDAYYAVDEEWRF
                     TYVNRKAEQLWGRSREELLGKNIWDEFPEAAGSESRRQIERAMEERVTTEFETVSPVL
                     GKHISGRAYPSAEGLSVYFQDVTGRKRAEESSRRLAAIVESSDDAIIAKTMEGRITSW
                     NDGAQRLYGYSAEEAVGKPITFLVPPEYPGEVAGILEKIRRGEKVDHFETVRVTKDGR
                     RLNISLSVSPIEDARGTIVGASTIARDVTERKKAEEELGRTLKELADIKFALDESAIV
                     AITDQRGRITYVNDKFCEVSKYSRDELIGQDHRIINSTYHPKEFIRDLWRTIARGRVW
                     RGELRNKARDGSIYWVDTTIVPFLNDRGKPYRYVAIRYETTERKRAEEEVRESNILLR
                     SVIEGTSDAVFLKDAWGRYLMVNSSAAEVIGLPVKDIIGKNDAELLAPEVGQALMEAD
                     LEVMTTGEARKIEEQVPVAGVTRTYLSTKAPYRDHEGRVIGVIGVSSDITGLKRAEEA
                     LREIREAERARISRELHDVVLQDLTYAMQVLHLAQTRRDGTASLDEAAAAMRRSVRGL
                     RSAVYDLSPEAEEGRFVRSVESLVDLNRGMNPECEYELNVGEGFPEELPERQSKELLR
                     IVQEALANARRHSAARRISVSAGASERELWVEVSDDGGGFDPKAVSAGVGTRGMRQRA
                     RALGGELEISSTPGGGTTVRLELAVETRRAQPAESGTDGARILLVDDHASFRQGVAAA
                     LEAEPGLEVVGQAGSLAEARAKLDGYDIAIIDLWLPDGFGGDLIRDLRAANPHAQVLV
                     LSSTEDRAEIARAVESGASGVMHKASELKEVADAVKRLRSGETLMPLEEVVDLLRFAG
                     ARRDEEHEARQAISRLTERELEVLSLLAEGLEAQEISSRLHISAKTERNHVASILFKL
                     GVHSRLQAVIFAWRHGLVEIGRKES"
     gene            5329..5709
                     /locus_tag="VHM69_15145"
     CDS             5329..5709
                     /locus_tag="VHM69_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741777.1"
                     /translation="MMGEKRTSAIERILHRIEEHVEEWRKRDAALQAEADQSRSQLWA
                     EAAERERMLAEAVGAEEARRESIEELTKEHRVVFVLHRKEVIETLEDFARQGDRLVSV
                     VPRRGGETISDGLKGSWLVFESSD"
     gene            5801..7723
                     /locus_tag="VHM69_15150"
     CDS             5801..7723
                     /locus_tag="VHM69_15150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX2741778.1"
                     /translation="MSERMITPYGSWRSPISSDLIVRGVVGLAQVTLDGNDVFWMESR
                     PGEGGRNVLVHRGADGVTEDVTPQPFNARTRVHEYGGGDFAVNEGTVYFSNFADQRIY
                     RRVPRGEPEPLTPDTGRRFADTVVDHDRRRLIAVREDHAQNGPINEIVGIDLETGEER
                     VLVSGNDFYASPRLSPDGARLAWLTWNHPNMPWDGTELWICELGADGSSENARQVAGS
                     HDESIFQPEWSPEGTLHFVSDRTGWWNLYREGRDGPLCEREAEFGLPQWVFGMSTYAF
                     VSPQRIVCSYTEHGNPRLAILDAETGELEPIETPYSSFAYLRASEATGAVLFRGGSPT
                     EPACIARLDPSTGQHEVLRRSGDLEIDTGYLSVPEPVEFPTQNVRTAHAFFYRPKNKD
                     YVAPKEELPPLLVMSHGGPTAAATPSLDPGIQYWTSRGIAVLDVNYGGSTGHGREYRR
                     RLDGMWGVIDVEDCANGASYLAERGLVDGGRLMITGGSAGGYTTLCALAFTDTFVAGA
                     SHFGVSDVEALARETHKFESRYLDRLIGPYPERADLYRERSPIHFADRLSCPVIFFQG
                     LEDEVVPPNQAETMFAALRKKGLPVSYVPFEGEQHGFRRAENIRRALDGELYFYSRVF
                     GFELADPVEPVPIENL"
     gene            7740..8549
                     /locus_tag="VHM69_15155"
     CDS             7740..8549
                     /locus_tag="VHM69_15155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741779.1"
                     /translation="MADWHDNWRDANRDHWDERVPIHVSGEFYDVGSFKAGQERLQPF
                     EIAEVGDVSGKDLLHLQCHFGIDTLSWARRGASVTGLDFSAPAIEEARKLASELGLEA
                     TFVHSDVYEAVKATGGRTFDVVYTGRGAILWLPDIRRWAEVVADLVRPGGFLYLTEFH
                     PFTEVFGDDLTVEHDYFQDENPRIWDEPGTYADFDAETSNNLTYEWNHTLGEVVSAVS
                     SAGLIVELLHEYDFTKFARWPFLEKSGFDTYRLPEGTPRLPLMYSLLARKL"
     gene            complement(8569..9147)
                     /locus_tag="VHM69_15160"
     CDS             complement(8569..9147)
                     /locus_tag="VHM69_15160"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2741780.1"
                     /translation="MWRPNRSLLVMAGLALILLSAGIVFLDVQRAGEESAAPPPKRDD
                     TMELTIPSMKRVKDVPVYTASAQNESALHDGTLHLKGTGFPWQEHANVYIAGHRLGYP
                     RTKSFLVFWDLNELRRGHRVVLTDSEHNRYIYRVYDRFILAPDDASAMKPVGGMDIVT
                     LQTCTLPNYKERLIVRAELERVLRPESQANAG"
BASE COUNT         1774 a         2959 c         3045 g         1600 t
ORIGIN      
        1 cggctaccgt agagtagccc ggctacggac gacacgagta acagcaccgc cagcaccccg
       61 gacagcgcgt aggagaaggt cagtctgttg ttgaccggta gcacagcgtc cttacccgtg
      121 ttccagcatc gtctcacggg tgagcactct caaaagctcc tcggtgaacc tctggaactc
      181 gtctatcagt tctgcgtcgt ggcaccggtc cctgacgatg tacgcggaga tcttcgcggt
      241 ctggcccgca cctgtgccat acgctatcga gacgtttgcc atcgccgttc tacctcccga
      301 ggatctaccc tgccagtgtc gtcttcacag ggtggccggg ccggaccgtg agacgacgac
      361 cgtggcttcc cgcgaggcgg tgatctccgc ctcgggcttc gagtagatga ggacaggacc
      421 ttgcgctgct ctcaggacgt tggtcgagac gctaccaagc ctcacgcggc ctatcacgcc
      481 gaggcccctg ctccccacgg agagcagagt cttgcgctcg tccccctcct cggccaccag
      541 caggagggcg agcgtggcgt ccgcgtcgac cacgcgcgtc tcagggcggc tgcccacggc
      601 ttccccgagt gcttctgccc gcctgttcag gtgctccgct tctcgcgacc tttgcttctc
      661 gagtcttcga cggtcctcgg cgctccagcc cccgatcggc tcgggcgggt tcctgtgggc
      721 ccgaacgagg acgtcttcgg ccccgaacat gcttccaatg ccggcggcca tctcagccgc
      781 aagccctgcg ctctccgagc cgtcgtcgcc ggtgatgatc ctatctggag gccaggactc
      841 gtctccccca cggaccacga gaacagggca cctcgcatga tggacgacgc cctcggagac
      901 gctaccgacg aagatgcgcc cgaccggccc gaggccacgg ctgcccatta cgaggagacc
      961 gggccggaga tcctcgcaga ggtccaggat ggcatctatg ggagggccgt gaaggaggtg
     1021 cggctcagcg acgaggtcac ccgtggcttc tatcgaatcc acctgtgccc cgagcaggcg
     1081 ccttgcctcc ttttcgtaga tgtgcgagta gtcggtccac accacggcag ggtaaccgag
     1141 accctgaatg tggtagtgcc aggcgtgtgc gacgtgcaac tcggatccgg tcttccgcga
     1201 gagatctgtg gcgacgcgcg cggcgagcat cgcgtccttc gagccgtcag tggccagcag
     1261 taccctgtct ggcctgatga caccgccgtg ctgactatcc atctcccgct ccttctccgt
     1321 gactcaggtt ctctatcctc acccgtccag caggcccgcc tcgcgtgccc ttgcaagcgc
     1381 ctcgagctgt gagtgggccc cgagcgccag gaggagggcg cgaatgtggt tcctgaccgt
     1441 ggcctgcgag aggtagagct cgcggcagat ctccctcgcc gactttccgg ccgcgagcaa
     1501 accgagaacc tcgagctgac gcggcgtgag caccggggtg tccctatggt tgcgaacggt
     1561 gcactcctca ggagcatcct ggcgggatag ccgtagcact tgtcgggcca tctccagcgt
     1621 ctcgtgcgcc gcctgcgtgt cgcgcaccat gtggaccagg tatagcccct ctccggtctc
     1681 cacggcgagg ctgctcacac cgacccaacg ctcccggccc gagcgggtga agacccgcac
     1741 ctcgtagccg ggtgcaggct gtccagccca ggcaagccgc acggccgagt acacgtgggc
     1801 gcagaacgag gcaccgtcct cgctctcccc ggttaggacc tcgtagcaga acccgccgac
     1861 catctcctca gcgagtagac ccgtgagcga ctccgccctg gcgtcccagt ggacgatgcg
     1921 aaactccggg tccactatga agacggcgtc cgcagtgagg gccccggtca agctttcgag
     1981 ggtctcggga agctccgcaa ctgctatctt atcgtgcgcc tcctgccctg tgctcagtgc
     2041 gaacgtcatt ctcgaccttt cttctagttc tgtcctgtgc ccgaatgatg ctccacgcca
     2101 tggatggggt catgagcagg gttggttatc ttcgcctggg ccggcggccc atgggcgtat
     2161 gggcaaaatt gcccacgttt tcgctgtcgt gatagacttt tcaggagagt gcggccgccg
     2221 gccagagaac cctcaggcaa agaatatatg tgatgatcct cggcgagcaa catgtgtcta
     2281 cccggaccgg accccgactt cttggcgcaa ttcgccacaa tgatggggct tttgctaccg
     2341 cagagcccga cgatataact tcatggtgac gcgaaagaca aatgaagggc gtgagcccgg
     2401 gctggagagt gagcgcgtgg cccacgacca gatggcgaac atcctcgaga gcatcagcga
     2461 cgcctactac gccgtcgatg aggagtggcg cttcacgtac gtaaacagga aggcggagca
     2521 gctctggggc cgttccaggg aagagttgct ggggaagaac atatgggacg aattccccga
     2581 ggctgcgggc tccgagtctc gcaggcagat cgagcgtgcc atggaagagc gtgtcacaac
     2641 tgagttcgag acggtctctc cggtgcttgg gaaacacatc tccgggcggg cctacccttc
     2701 tgcggaggga ctctctgtat acttccagga cgttaccggg cgcaagaggg cagaagagag
     2761 cagccgcagg ctcgcggcga tagtcgagtc ctcggacgac gccatcatcg ccaagaccat
     2821 ggaaggcagg atcaccagtt ggaacgacgg agcgcagagg ctatacggct actctgccga
     2881 ggaagccgtg gggaagccca tcactttcct tgttccgccc gagtaccctg gcgaggtggc
     2941 cgggatcctc gagaagatca ggcgtggaga gaaagtcgat cacttcgaga ccgtgcgtgt
     3001 gaccaaggac ggcaggaggc tgaacatatc cctgagcgtg tctcccatag aagatgcccg
     3061 cggaacgatc gttggcgctt caacgatagc cagggacgta accgaacgca agaaggccga
     3121 ggaagagttg ggcagaacgc tcaaggagct cgccgacatc aagttcgccc tcgacgagag
     3181 cgccatcgtc gcgatcaccg accagcgagg caggatcacc tacgtcaacg acaagttctg
     3241 cgaggtctcc aagtactcga gagacgagct catcgggcag gaccaccgca tcataaactc
     3301 cacctatcat ccgaaggagt tcatcaggga cttgtggagg acgatcgcgc gtggcagggt
     3361 gtggcgcgga gagctgagga acaaggccag ggacggttcg atctactggg tggacacgac
     3421 catcgtccct ttcctgaacg accggggcaa gccctaccgc tacgtcgcga tccgctacga
     3481 gaccaccgag cgcaagcggg ccgaggagga ggtgcgggag agcaacatcc tcctgcgttc
     3541 cgttatcgag gggacgagcg acgccgtctt cctgaaggac gcgtggggcc gctacttgat
     3601 ggtgaactcc tccgctgctg aggtcatcgg gctgccagtc aaggacataa tcggcaagaa
     3661 tgacgccgag ctgctcgccc ccgaggtcgg ccaggcactc atggaggccg acctcgaggt
     3721 catgaccacg ggggaggccc gcaagatcga agagcaggtc cccgtcgccg gtgtcacgcg
     3781 cacctatctc tcgaccaagg cgccgtaccg ggatcacgag gggagggtga tcggcgtcat
     3841 cggcgtctct tcggacatca cagggctcaa gcgcgccgag gaggcgctgc gcgagatcag
     3901 ggaggcagaa cgcgcccgca tctcccgcga gctacacgac gtcgtcctcc aggacctcac
     3961 ctacgcgatg caggtgttgc acctcgccca gactcgccga gacgggactg ccagcctgga
     4021 cgaggcagct gcggccatgc ggcgatctgt ccgcgggctg aggagcgccg tctacgacct
     4081 gagcccagaa gcggaggaag gacggttcgt ccgctccgtg gagtctctcg tcgacctcaa
     4141 caggggaatg aaccccgagt gcgagtatga gctgaacgtc ggcgaaggct tccccgagga
     4201 gctaccggaa aggcaatcca aagaactgct gcgcatcgtg caggaggcgc tggcgaacgc
     4261 caggcgtcac tccgcggcgc gacgcatctc ggtctccgca ggggcttcag aaagagaact
     4321 ctgggtagag gtctccgacg acggaggtgg attcgacccg aaagcggtct ccgccggggt
     4381 gggcacgagg gggatgagac agagggcaag ggcgctgggc ggggaactgg aaatatcgag
     4441 cacgcccggc ggaggtacga ctgtacgact cgagttggct gtagagacgc gtcgagcgca
     4501 gccagcagag tcaggtacgg acggagcccg gatactgctc gttgacgacc acgcctcctt
     4561 ccggcagggc gtcgcggccg cgctggaggc tgagcctggg ctcgaggtcg tcgggcaggc
     4621 cggatctctc gccgaagcgc gggcgaagct cgacggatac gacatcgcta tcatcgacct
     4681 ctggctaccc gacggcttcg gcggcgacct gatccgggac ctccgggccg ccaacccgca
     4741 cgcacaggta ctggttctca gctccacgga agaccgggcg gagatcgcgc gggcggtcga
     4801 atcgggggca tcgggggtca tgcacaaggc ttcggagttg aaggaggtgg ctgatgcggt
     4861 caagcgcctt cgctcgggcg agacgctgat gcccctcgaa gaggtcgtcg atctccttcg
     4921 cttcgccggc gcacgcaggg atgaagagca cgaggcgcgg caggcgatct cacgtctcac
     4981 cgaacgagaa ctagaggtgc tctcgctcct cgcggagggc ctggaagcgc aggagattag
     5041 ctccagattg cacataagcg ccaagacgga gcgcaaccac gtcgcgagca tcctcttcaa
     5101 acttggcgtt cactcccggc tgcaggctgt catcttcgcc tggcgccatg gcctcgtcga
     5161 gataggcagg aaggagtcct aggcgcttcg tgagatatcg acactcttag ccctggtctc
     5221 cgtagcgatt tccccggacg agggctactc aacggccagg gatcgggcgc gggaagcgaa
     5281 acaagggtac agtgaatagt actcgggaca cgccacgtaa ggaggttcat gatgggcgag
     5341 aagcgtacat ccgccataga gcggatactg cacaggatcg aggagcacgt ggaggagtgg
     5401 cgcaagcggg acgccgccct ccaggccgag gccgaccaga gccggagcca actctgggcc
     5461 gaggcggcag agagggagcg aatgctggct gaggccgtgg gagctgagga ggcgcgacgc
     5521 gagtccatcg aggagctcac gaaggaacac agggtcgtct tcgtcctgca ccgcaaggag
     5581 gtgatcgaga cgctcgagga cttcgcccgt cagggcgaca ggctcgttag cgtggtgccc
     5641 cgcagagggg gtgaaacgat aagcgacggc ctgaagggct cgtggctggt cttcgaatcc
     5701 tccgactgaa gggcgcagcg gtccccggaa gattcgcggg tgggagtatg ctcgcacccc
     5761 tcaatacgga acctttcaac tggtgcggag gtaccgagtc atgtccgaac gcatgatcac
     5821 gccctacggt tcttggaggt ccccgatctc ttcggacctg atcgtccgtg gcgtcgtggg
     5881 cctggcgcag gtgacgctcg acggcaacga cgtattctgg atggagagcc gccccggcga
     5941 gggcgggcgc aacgtgctcg tgcaccgcgg agcggatggg gtcaccgagg acgtcacgcc
     6001 gcaaccgttc aacgcgcgca cgcgggtaca cgagtacggc ggaggcgact tcgccgtcaa
     6061 cgaaggcacg gtctacttct cgaacttcgc cgaccagcgg atctaccgcc gagtcccccg
     6121 cggcgagccc gagcccctta cgcccgatac aggcaggcgc ttcgctgaca cggtggtcga
     6181 ccatgaccgc cggcgtctga tcgccgtgcg cgaggatcac gcgcagaacg gcccgataaa
     6241 cgagatagtc ggtatagacc tcgaaactgg agaggagcgc gtgctcgtct ccggcaacga
     6301 cttctacgcc tcgccacgcc tgagccccga cggcgcgcgc ctcgcgtggc tgacgtggaa
     6361 ccatccgaac atgccgtggg acggcacgga gctgtggata tgcgaactcg gcgcggacgg
     6421 gtcttccgag aacgccaggc aggtcgcagg cagtcacgat gaatccatct tccagccgga
     6481 gtggtccccc gagggcacac tccacttcgt ctctgaccgc acaggctggt ggaacctgta
     6541 ccgtgaggga cgggacgggc cgctctgcga gagggaggcc gagttcggcc tgccgcagtg
     6601 ggttttcggg atgtccacgt acgctttcgt ctcaccgcaa cgcatcgtat gctcctacac
     6661 ggagcacgga aaccctcgcc tcgccatcct cgatgctgag acaggagagc tggagcctat
     6721 cgagacgccg tactccagct tcgcgtacct gcgcgccagc gaagcaacgg gcgctgttct
     6781 cttccgcggc ggatctccga cggagccggc gtgtatagcg aggctggacc cctctaccgg
     6841 ccagcacgag gtcctgcgcc ggtcgggcga cctcgagatt gacactggct acctctccgt
     6901 tcccgagcct gtagagttcc ccacgcagaa cgtccgcacg gctcacgcct tcttctatcg
     6961 gccgaaaaac aaggactacg tcgctccgaa agaagagctg ccgccgctcc tcgttatgag
     7021 ccacgggggc ccgaccgcgg cggcgacgcc ctccctcgac cctgggatac agtactggac
     7081 cagccgcggc atcgccgtgc tcgacgtgaa ctacggtggg agcacgggcc acggacgcga
     7141 gtatcgtcgc cgactcgacg gtatgtgggg cgtcatcgac gtcgaggact gtgccaatgg
     7201 ggcgagctat ctcgcggagc gcggcctggt ggacggcggg cggctcatga tcaccggcgg
     7261 cagcgccggc ggctacacga cgctgtgcgc gctcgcgttc acggatacgt tcgttgcggg
     7321 cgcgagccac ttcggggtca gcgacgtgga ggccctggca agggagaccc acaagttcga
     7381 gtcgcgctac ctcgaccggc tcatcggacc ctaccccgag cgcgccgacc tctacaggga
     7441 gcgctccccg atccacttcg cagaccgtct ctcctgcccc gtgatcttct tccagggcct
     7501 cgaagacgaa gtcgtgcccc cgaatcaggc ggagacgatg ttcgcggccc tgcgaaagaa
     7561 gggcctcccc gtctcatacg tccccttcga gggagagcaa cacggtttca ggcgcgccga
     7621 gaacatcagg cgggcgctcg atggcgagct gtacttctac tcgcgcgtct tcggcttcga
     7681 gctcgcagac cctgtcgagc cggtaccgat agagaatctg tagagacggg aggagatcta
     7741 tggctgactg gcacgacaac tggcgggacg cgaacaggga ccattgggac gagcgcgtcc
     7801 cgatccacgt ctccggcgag ttctatgacg taggatcgtt caaggctggt caggagcgtc
     7861 tgcaaccttt cgagatagcc gaggtcggcg acgtgtcggg taaagacctt ctgcacctgc
     7921 agtgccactt cgggatcgac accctgagct gggcccgccg cggcgccagc gtgacgggcc
     7981 tcgacttctc cgcccccgcg atagaggagg cccggaaact cgcttcggag ctggggcttg
     8041 aagcgacctt cgtgcactcc gacgtctatg aggcggtaaa agccacgggc ggccgtacct
     8101 tcgacgtcgt ctacacgggc cggggagcga tcctctggct ccccgatata aggcggtggg
     8161 ccgaggtggt cgctgacctc gtcaggcctg gcggcttcct gtacctcact gagttccacc
     8221 ctttcaccga ggtcttcggg gacgacctga ccgtggagca cgactatttc caggatgaga
     8281 accccaggat atgggacgaa ccaggcacct acgccgattt cgacgccgag acgtcgaaca
     8341 acctgaccta cgaatggaat cacaccctgg gcgaggtggt gagcgcagtg agctcagccg
     8401 gcctcatcgt cgagctgctg cacgagtacg acttcacgaa gttcgcgcgg tggcctttcc
     8461 tggagaagtc agggttcgac acctaccgtc ttcccgaggg cacaccgcga ctccctctca
     8521 tgtactcgtt gctggcgcgc aagctgtaag ggcggttagc gaaccgccct atccggcgtt
     8581 ggcttgagat tccggtctca agaccctttc gagctcggcg cgtacgatca ggcgctcctt
     8641 gtagttcggg agcgtgcagg tctggagggt gactatgtcc atccccccta ccggcttcat
     8701 ggctgaggca tcgtcaggcg ccaggatgaa acggtcgtag accctgtaga tgtatctgtt
     8761 gtgctcggag tccgtcagca caaccctgtg cccgcgcctc aactcattga ggtcccagaa
     8821 gacgaggaag ctcttggtgc gcgggtagcc aagcctgtga cccgcgatgt agacgttggc
     8881 atgttcctgc caggggaagc ccgttccctt gaggtgcagc gtcccgtcgt gcagcgccga
     8941 ctcgttttgc gccgaggcgg tgtacacggg aacgtccttc acccgcttca tgctggggat
     9001 ggtcagttcc atggtgtcgt ccctcttcgg cggcggcgcc gccgactcct cccccgccct
     9061 ctgaacgtcg aggaaaacga tacccgccga gagcaggatc agggcgagtc ccgccattac
     9121 aagcaggctg cggttaggcc gccacatggt cctgtagctc ggcgttcttt tccgttctcg
     9181 ggtccacgcc ccgtatggtt tggttctcac ggtggactat gatacatccc cagcgcaaca
     9241 aaacaacacg aagcgttaac aatcgttcgc gaggacgaca ccaggaggct acctagtgcg
     9301 gcggggatcg agagcgtcgc gcagtccgtc gccgataaaa ttgacggcga gaaccgagag
     9361 ggctatggcg atgcctgg
//
LOCUS       DATAIR010000639         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_44014, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000639 DATAIR010000000
VERSION     DATAIR010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44014"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..439)
                     /locus_tag="VHM69_15165"
     CDS             complement(200..439)
                     /locus_tag="VHM69_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741781.1"
                     /translation="MRKKLTILVAMLAMMMFAAVPAFANTTLVNIGPLNAEGEGVISA
                     EAESVFLGNDGAEGKIGGFTVEAESHFLFFHFNDE"
     gene            678..2063
                     /locus_tag="VHM69_15170"
     CDS             678..2063
                     /locus_tag="VHM69_15170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX2741782.1"
                     /translation="MPVENFEVGVIGAGYVGLTTGACLAHLGHRVTCLDNDEERVTSL
                     AEGRVPFYEPGLDDLVTSTASAGRLSFASTERLAGVVQGADVVFIAVGTPQGDDGSAD
                     LSNVSSVARQVGRSLAASPPRGRPLVVVNKSTVPIGSGDYVSMLIEEGAGDEGVADFL
                     VVSNPEFLREGSAVYDTLFPDRIVVGADSREPLETMRMLYEPIIGQTFPTQIDPRPKA
                     AVPFVITDLASAEMIKYAANAFLATKISFINEISNLCELTGADVGRVAKGIGLDGRIG
                     MRFLNPGIGWGGSCFPKDVSALRAAALEYQHETPLLDATVAVNERQRGRVIQKLQSEL
                     HTLKGKRVALLGLAFKPNTDDMRGAPSVEIARALGAMGARTVGYDPVAGKAAATVMPD
                     LRVVFEPYEALDGAHAAVLVTEWEEFRRIDLPRAAALMREPRLLVDGRNALEPGLVRK
                     AGLKYTGFGRG"
     gene            2056..>2739
                     /locus_tag="VHM69_15175"
     CDS             2056..>2739
                     /locus_tag="VHM69_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2741783.1"
                     /translation="MGERPRALVTGGAGFLGSHLCERLLAEGYGVVCVDNLSTGVRAN
                     VAHLEEDPNFEYVEHDVTTPFEAGGRLDEVYHFASPASPADFERIPIQILEAGAFGTH
                     NALELARSVGARFMLASSSEVYGDPLVHPQYEDYRGNVSTTGVRSVYDEAKRYAEAAT
                     MAYHRHHGLDTRIVRIFNAHGPRMRPDDGRMIPAFVSQALAGRPLTVYGDGNQTRSLQ
                     YVDDLVEGVF"
BASE COUNT          503 a          870 c          861 g          505 t
ORIGIN      
        1 cagcgacctc tccttcgagg agatggtgcg cctcgacctc tactacatag agaactggtc
       61 gctctcgttc gacctcaaga tcatcctgaa gaccctcggc gccgttctac gacgcgaagg
      121 cgcgtactga tcagaccgta ccgcggtctc cagcgccgcg gggtggggtg actccccacc
      181 ccgcagcgct gagactttcc tactcgtcgt tgaaatggaa gaacaggaag tggctttcgg
      241 cttcgacggt aaacccgcct atcttgccct ctgcgccgtc gttaccgagg aagacactct
      301 cggcttcggc gctgatgacg ccttcgcctt cggcgttgag cggaccgatg ttgacgagcg
      361 tcgtgttcgc gaacgccggg acggcggcga acatcatcat cgccagcatc gctaccagta
      421 tagtcagctt cttcctcatc tctctccttt acgattcccc gatattgctg attcgtcacc
      481 gcctggcaac tctcctatcg tacccccttt gtgaaagtat ctttccctga tgtggaattc
      541 tataccttcc cgcacgataa gcaatgtatt ttgcttgatt gccaggatac gctccgcctg
      601 tagtaagcta cgaagcgtaa tctccaggtc gatgctgaca acgttcgggg tggcaccgac
      661 ccagatcagg aggcgttttg cccgtcgaga acttcgaggt aggggttata ggagccggtt
      721 acgtaggatt gacgacgggc gcgtgcctgg cgcacctcgg gcacagggtg acctgcctgg
      781 ataacgatga agagcgcgtg acgagcctcg cggaggggcg agttcccttc tacgagcctg
      841 gtctcgacga tctggtgacc agcactgcga gcgcgggacg actctctttc gcaagcactg
      901 agaggctggc cggggtggtg cagggggctg acgtggtttt tatcgccgtg ggaacgccgc
      961 agggcgatga tggatcggcc gacctctcga acgtgtcgag cgtggcacgc caggtagggc
     1021 gctccctcgc cgcgtctccg ccccgcgggc gccctctggt cgtggtcaac aagagcaccg
     1081 tgccgatagg gagcggtgac tacgtatcga tgttgatcga ggaaggtgct ggcgacgagg
     1141 gtgttgccga cttcctcgtg gtctcgaacc ccgagttcct cagagagggg agcgccgtct
     1201 acgatacgct tttccctgac aggatcgtgg taggggcgga ctctcgcgag ccgctcgaga
     1261 ccatgcgaat gctctacgag ccgataatcg ggcagacctt ccccacacag atagaccccc
     1321 gccccaaagc tgccgtgccg ttcgtcataa cggaccttgc gagcgcggag atgatcaaat
     1381 acgccgcaaa cgcgtttctc gcgaccaaga tcagcttcat aaacgagatc tccaacctct
     1441 gcgagctgac aggggccgac gtaggcaggg tggcgaaagg catcggcctc gacgggcgca
     1501 tcgggatgcg attcttgaac cccggcatcg gatggggtgg gtcttgcttc cccaaggacg
     1561 tctctgccct gagggcagcg gcgctcgagt accagcacga gacgccgctg ctcgacgcga
     1621 ccgtagccgt caacgagcgc cagcgcggga gggtgatcca gaagctccag agcgagctcc
     1681 acaccctgaa gggcaagcgt gtcgccctcc tcgggctcgc cttcaagccg aacacggacg
     1741 acatgagggg ggccccgagc gtcgagatag cccgtgccct gggcgcaatg ggagcacgca
     1801 ccgtcggcta cgatcccgtg gccggcaagg cggcagcgac ggtcatgccc gacctcaggg
     1861 tcgtcttcga accttacgag gccctcgacg gcgcgcacgc cgcggtgctc gtgacggagt
     1921 gggaggagtt caggcgcata gatctccctc gcgccgccgc gcttatgcgg gagcccaggc
     1981 tcctcgtgga cgggcgcaac gcgctcgagc ccggcctcgt gcgcaaggcg ggcctcaaat
     2041 acaccggctt cggccgtggg tgagagaccc cgcgcgctcg ttaccggcgg ggccggtttc
     2101 ctcgggagtc acctctgcga gcggctcctc gctgagggct acggcgtggt atgcgtggac
     2161 aacctgagta cgggtgtgcg ggcgaacgtg gcgcatctgg aagaagaccc caacttcgag
     2221 tacgtcgagc acgacgttac gacgcccttc gaggccgggg gccggctgga tgaggtatac
     2281 catttcgcct cccccgcgag ccctgcggac ttcgagcgca tccccataca gatcctcgag
     2341 gcaggcgctt tcggcaccca caatgccctc gaactcgcgc gctcggtggg cgcccgcttc
     2401 atgctcgcct cgtcctcaga agtctacggc gacccgctcg tgcatccgca gtacgaggac
     2461 tatcggggca acgtcagcac cacgggcgtc aggagcgtct acgacgaggc caaacgctac
     2521 gcagaggccg ccaccatggc ctaccaccgt catcatggct tagatacgcg catagtgcgc
     2581 atctttaatg cccacggccc gaggatgagg cccgacgacg ggcgtatgat ccccgccttc
     2641 gtctcccagg ctctcgctgg gaggcccttg accgtgtacg gagacggcaa ccagacaagg
     2701 agcctgcagt acgtagacga cctcgttgaa ggggtcttt
//
LOCUS       DATAIR010000640         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_136911, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000640 DATAIR010000000
VERSION     DATAIR010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136911"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..678
                     /locus_tag="VHM69_15180"
     CDS             334..678
                     /locus_tag="VHM69_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741784.1"
                     /translation="MRILVANTPRLYRESLALSIQQHRPQYEVLLADPEALDGHVERL
                     SPHALVRDDDGIETEAPDGVICWAGINIDDHLNARISVNGRISEMHDASLEEFLAALD
                     EAEGLISTDGGK"
     gene            complement(696..1385)
                     /locus_tag="VHM69_15185"
     CDS             complement(696..1385)
                     /locus_tag="VHM69_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741785.1"
                     /translation="MEGRSKTGAIRVLLADDHTMFRQGIAGVLDEYGGMEVVGQTSND
                     EGAVEMARRTKPDVVLMQVQMPIQRAKENLRLMREVEPPPKVVIVTMIEDPRHVREIM
                     DVGVSAYIVKSASAEHLVAAVRAAILDPKSKNVVVGMPLGLLEKAERGSQGVLSAREL
                     EILLLAARGLSNRQIANSLHVAEGTVKRHLANTFEKMDVRSRGEATRMALHEEWITIQ
                     DVTDGLEGESG"
     gene            1768..2838
                     /locus_tag="VHM69_15190"
     CDS             1768..2838
                     /locus_tag="VHM69_15190"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX2741786.1"
                     /translation="MNLDRPYQDETPGEIESPETYSRANQYAELKRLVRQEGLLDQQP
                     AYFAGKSILTFGLLAVSLALLLVSGNTWLQLLNAAYLAFVFVQISLLAHDCGHRQISF
                     RAPWKNDLLTLVLGNLLLGMSRQWWIDKHNEHHGHPNQIDVDPDIDIPLLAFEEEQAL
                     DKRGIARFVVKYQAILIFPLSLLQALSMLRSSIGFLVARKAKSALAEVLAMGAHFALY
                     FGLLFSTLEPLQAVLFIIVHRGLYGLYMVSIFAPNHKAMPLLEQDSEEDFLRRQVLTS
                     RNVFAHPITDFWYGGLNYQIEHHLFPRMPRNNLREAQPIIKDFCRVHSIAYHETSVLQ
                     SYREILQHLHEVGAPLRKGSAT"
     gene            complement(2914..3795)
                     /locus_tag="VHM69_15195"
     CDS             complement(2914..3795)
                     /locus_tag="VHM69_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2741787.1"
                     /translation="MATKAVIIGNPNSGSAGDEGYLERFAETLREGGLEVEVLNTERP
                     DHATELAAEAGDQLVVAAGGDGTVNEVVNGLSKDATLGILPLGTANVLARELGLPLEP
                     EDACKRILTGTGTRMDVGVATDEEGTERRFTCMAGIGFDANVVREVTPRFKRYLRSLA
                     FPLAALKVYLQGDQPDLQIVDGDTTYVTQFAVVANGQYYGGEFEMAEEASLGSGALEV
                     VLIEKVGLLLRPDVLARILAKSPLDRAMKSFSSQGILAKSPAARGVRVPVQIDGEVWG
                     SLPMSFRIEPAAISVIR"
BASE COUNT          741 a         1299 c         1175 g          802 t
ORIGIN      
        1 atccccacaa gagccacacc cccgagaacc cggcttggag caagaatatc gtaggaacga
       61 aaacgcccgt tgagttccgg tgcctgggac cgggccattc gccgtgaaat accccgccgg
      121 aaccgccttc caacacgcgg aaggtcatac gcctttcggc ccaagcctac gcatttgggg
      181 ccatttttgg cgcagcgggc acaaatgctt acgaaagggc taggatacgc caccgaaagt
      241 tgtaagcttg gtgtatacac cctaacgggt aggcgacatc tgcgtgtgtt ctttcatgtg
      301 ggctttgtct gcacagagag gagcacatgt ttcgtgcgaa tattggttgc gaatacgccg
      361 cgattgtacc gggagtccct ggctctctcc atccaacagc accggcctca atacgaggtg
      421 ctgcttgccg accccgaggc tctagatggg catgtggagc gtctgtctcc ccacgcgctc
      481 gtgcgagacg acgacgggat cgaaacggag gcgccggacg gcgtcatctg ctgggccggg
      541 atcaacatcg acgaccacct caacgcgcgc atcagcgtga acgggcggat ctcggagatg
      601 cacgacgcga gcctggagga gtttctcgcg gccctggacg aggctgaggg gttgatctcc
      661 accgacggcg gcaagtagga acgcttcccg gagttctagc cgctctcccc ttcgaggcca
      721 tcggtgacgt cctggatggt gatccactcc tcgtgaagag ccatcctggt ggcttcgccc
      781 ctggatcgca cgtccatctt ctcgaaggtg ttggcgagat ggcgcttgac ggtcccctcc
      841 gccacgtgca aggagttcgc aatctggcgg ttgctgagac ccctggcggc caggagcagt
      901 atctctagct cccgggcgga gagcacgccc tgcgagcctc tctcggcttt ctccagcaac
      961 ccaagcggca tgcccaccac gacgttcttg ctcttcgggt ccaggatcgc ggcccgaacc
     1021 gccgccacca ggtgctcggc cgaggcgctc ttgacgatgt aggcgctcac cccgacgtcc
     1081 atgatctccc tgacgtgccg cgggtcctcg atcatcgtga ctatgacaac cttcggcggc
     1141 ggttcgacct cgcgcattag ccggaggttc tcctttgccc tctggatcgg catctggacc
     1201 tgcatgagca ccacgtcggg cttcgtccgg cgggccatct ccacggcccc ctcatcgttg
     1261 ctggtctgcc cgacgacctc catgccgccg tactcgtcca gaacccccgc gataccttga
     1321 cgaaacatag tgtggtcgtc tgccaggagc accctgatag cgccggtttt gcttcttccc
     1381 tccatttcct cgctttcagg gttatgccct ctggctggga gagctaactg taataacatt
     1441 ttcatcttat ccaaacgctc tgactccgac aacatagctt ggggagcata ctctcgccaa
     1501 ccgcactccg ggctcgccct cggcgagcgc cgcgccgcct agtatgaagc ttactcggtg
     1561 catatttcga tacatttacg ccgttaaaca gccgaatcag agtttgagac ggtccggagt
     1621 ggctatattc tcgtcacccg tcttctaaaa cgtgcacgga ctacatacct tctcgtcggt
     1681 tctcaaaaga agtgagaacg aaggtctgat gctgaagcgc gaaccagtcg gatcgggcag
     1741 cacctagatt ggaaaggaac atacttcgtg aatttggatc ggccgtacca ggacgagacg
     1801 ccgggcgaga tcgagagccc cgagacctac tctcgggcca accagtatgc cgagttgaag
     1861 cgtctcgtca ggcaggaagg gctcctggat cagcagcccg cttatttcgc gggcaagagc
     1921 atactgacct tcggcttgct ggcggttagc ctggccttac tgctggtctc gggcaacacc
     1981 tggctccagc ttctgaacgc cgcctatctg gccttcgtct tcgttcagat cagcctcctc
     2041 gcccacgact gcgggcaccg gcaaatctcc ttccgcgctc catggaagaa cgacttgctc
     2101 accctcgttc tggggaacct cttgctcggc atgagccgcc agtggtggat cgacaagcac
     2161 aacgagcacc acggacatcc gaaccagatc gacgtcgacc ccgacatcga catcccgctg
     2221 ctcgccttcg aagaagagca ggctctggac aagcggggga tcgcgcggtt cgtcgtgaag
     2281 tatcaggcta tcttgatctt cccgttgtcg ctcctgcagg ctctgagcat gctccgcagc
     2341 agcatcgggt ttctcgtggc gaggaaggcg aagagcgctc tggccgaggt gctagccatg
     2401 ggagctcact tcgctttgta ctttggcttg ctgttctcta cgttggaacc cttgcaggcg
     2461 gtgctgttca taatcgttca ccgtgggctc tacggtttgt atatggtctc gatcttcgcg
     2521 ccgaaccaca aagccatgcc tcttctggag caggatagcg aggaagactt cctgcgccgg
     2581 caggtgctga ccagccggaa cgtcttcgcg cacccgatca ccgacttctg gtacggcggc
     2641 ctcaactacc agatcgaaca ccaccttttc ccaaggatgc cccgcaacaa tctgcgggaa
     2701 gcgcagccga tcatcaagga cttctgccgt gttcactcga tcgcttacca cgagaccagc
     2761 gtgctgcaat cctaccggga gatcctgcaa cacctgcacg aggtcggcgc tccgctccgc
     2821 aagggatcag cgacataacg gctccggtcg gacgcgcata gaacaaggcc ggccgctgga
     2881 ggaaagtacg cccggttacg cagccgcccg tggctacctg atcacgctta tagccgccgg
     2941 ttcgatgcgg aaagacattg gaagacttcc ccagacttcg ccgtctatct gcaccgggac
     3001 gcgcacgccg cgagcggccg gggatttggc caggatgccc tgcgacgaga acgacttcat
     3061 cgcgcggtcc agggggctct tggccaggat tcgggccagg acgtcgggcc tcaggagcag
     3121 gcccaccttc tcgatgagga cgacctccag cgcgccgctc cccagggagg cctcttcggc
     3181 catctcgaat tctcctccat agtattggcc gttggcgacg acggcgaact gggtcacgta
     3241 ggtcgtgtcc ccgtccacga tctgcaggtc cggctgatcg ccctggaggt acaccttgag
     3301 cgcggccagc gggaaggcga gcgacctgag gtagcgtttg aagcggggcg tcacctcgcg
     3361 gacgacgttc gcgtcgaagc cgatgccggc catgcaggtg aagcgccgct ccgttccctc
     3421 ctcgtcggtc gccaccccga cgtccatccg cgtgccggtt ccggtcagga ttcgcttgca
     3481 agcgtcctcc ggctccagcg gcagcccgag ctcccgcgcc agcacgttcg ccgtgccgag
     3541 cggcaggatg cccagggtgg cgtctttgga gaggccgttc acgacctcgt tcaccgtccc
     3601 gtcacccccg gccgcgacta cgagctggtc gccagcctcg gcggcaagct ccgtggcgtg
     3661 atccggacgc tcggtgttga gcacctcgac ctccaggccg ccctcgcgca gggtttcggc
     3721 gaagcgctcg aggtagccct catcccccgc gctcccggaa ttggggttgc cgatgatgac
     3781 cgctttcgtt gccatacagt tagctccgtc cttttcgggt gttaccacgt cgcgacgaac
     3841 cggcctgcca tccttacccc ggcatcagac agtacgatcc tgcacggcag gctccgagat
     3901 ggcaacttgc cgggtatctc aaccggtgcc cgtataagca aggggtgata ggtggggaaa
     3961 ggaaaggagg atacgggctc cggttgcgtc aatagtcagc tacgcttcta tgcctgt
//
LOCUS       DATAIR010000641         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_44126, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000641 DATAIR010000000
VERSION     DATAIR010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44126"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VHM69_15200"
     CDS             complement(<1..543)
                     /locus_tag="VHM69_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741788.1"
                     /translation="MRIAVDALGGDNAPREIVAGAVSAARRLPRDEILLVGKLDEIKA
                     LLGANPPENISLRSAGSSVGMEEEPAAALKAHPDASVAVAAEMVRDGDADALFSAGNT
                     GATVAAALLRIGRLEGCRRPAIATMLPFPTPVLLLDAGATVACRPQDLMNFAILGGVF
                     AKRYFGLEDEARVGLVNVGEE"
     gene            complement(575..757)
                     /gene="rpmF"
                     /locus_tag="VHM69_15205"
     CDS             complement(575..757)
                     /gene="rpmF"
                     /locus_tag="VHM69_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2741789.1"
                     /translation="MAVPKKKTSKARRNQRRAHHAITGPGLVACPNCGEPHVPHRVCQ
                     ECGYYKGRTVVAVEGI"
     gene            complement(785..1243)
                     /locus_tag="VHM69_15210"
     CDS             complement(785..1243)
                     /locus_tag="VHM69_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741790.1"
                     /translation="MNHLIPLRRPGAEVGDRHDVRAEFSVDPFDLYGRHHEVTKAEAD
                     VGVTRLSEGLHLDVQVTATVLTTCDRTLEPTELKLEFGDSEFLSGPGNPEIAVEDWTL
                     DLARYTERALPNEVPMQVFSPGTEPVQPEGDGDEVDPRWHGLDDVFASGF"
     gene            complement(1263..>1512)
                     /locus_tag="VHM69_15215"
     CDS             complement(1263..>1512)
                     /locus_tag="VHM69_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2741791.1"
                     /translation="SDRIIRSAQDEAEKITSEEEVLKRAEKRSAEIMEDARRREREIR
                     LGAEDYADEVLANLEDNLGKLLEAVQRGRTKLQGVQEQ"
BASE COUNT          236 a          523 c          464 g          289 t
ORIGIN      
        1 ctcctcaccg acgttgacga gtccgacccg cgcctcatcc tcaagtccga aataacgctt
       61 cgcgaacacg ccaccaagaa tagcgaaatt catgaggtct tgcggcctgc aggcgaccgt
      121 ggccccggcg tcgaggagca atacaggcgt cgggaaaggc agcatcgtgg cgattgccgg
      181 cctgcggcac ccctccaggc gtccgatccg cagcagcgcc gccgcgaccg tcgcgcccgt
      241 gttcccggct gagaatagcg cgtcggcgtc cccgtcgcgc accatctcgg cagcgacagc
      301 gacgctcgcg tcgggatggg ccttgagcgc cgccgccggc tcctcttcca ttcctacgga
      361 cgaacccgcg gacctcagag aaatgttctc gggggggttg gcacccagca gtgctttgat
      421 ctcatcgagt ttgccgacga gcaggatctc atcccgcggc aacctccggg cggcggagac
      481 ggcgccggcg acgatctctc gcggggcgtt atctccgccg agggcgtcta ccgcgatacg
      541 cactggtcgg tctatctagt tttcggggct gccgctagat gccctccacg gcgaccaccg
      601 tgcggccctt gtagtaaccg cattcctggc agaccctgtg cggaacgtgc ggctcgccgc
      661 agttcgggca cgcgacgagg ccggggcccg tgatcgcatg gtgagcccgg cgctggtttc
      721 tgcgcgcctt cgacgtcttc ttctttggta cggccatgag attacctcct ggttgcttgg
      781 aagattaaaa ccccgaggcg aaaacgtcgt caaggccgtg ccagcgcggg tcaacctcgt
      841 cgccatcacc ctcgggttgc acgggctccg ttccaggcga gaaaacctgc atcggaacct
      901 cgttcggcag ggcacgctcc gtgtagcgcg ccagatcgag agtccagtcc tccacggcga
      961 tctcagggtt gcctggccct gagaggaact ccgagtcgcc gaactccagt ttcagctccg
     1021 tgggctccag cgtcctgtcg cacgtcgtca gcacagtcgc cgttacctgc acgtcaaggt
     1081 gcagaccttc agagagtcgc gtgacgccaa cgtcggcctc agccttcgtc acctcgtggt
     1141 ggcgtccgta gaggtcgaag ggatctacgg agaactcggc acgcacgtcg tgcctgtctc
     1201 caacctccgc gcctggccgc cttaagggga tcaagtggtt catgcggcta gtttactacg
     1261 ggctactgtt cctggacgcc ctgcagcttc gtacggcccc gctgcacagc ctccaggagc
     1321 tttccgaggt tgtcctccag gttcgcgagg acctcgtcag cgtagtcctc cgcgcccagc
     1381 cggatctcgc gctcgcggcg gcgggcgtcc tccatgatct ccgcgctccg cttctcggcg
     1441 cgcttcagga cctcttcctc ggaggtgatc ttctccgcct cgtcctgggc gctgcggatg
     1501 atacggtcgc tc
//
LOCUS       DATAIR010000642         5476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_250834, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000642 DATAIR010000000
VERSION     DATAIR010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250834"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1331
                     /locus_tag="VHM69_15220"
     CDS             93..1331
                     /locus_tag="VHM69_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741792.1"
                     /translation="MTEGTSAQGRGEDPGGISTRTASWLAWSLCGATVLILALSLLLI
                     LLGWSTPQPRGEVPWRNQAISLVGLVGAPILGGLIASRLPRNPYGWVWLGFGLGLTLQ
                     QLAASYTAYARVVEPGSLVAPRAISHVLALGGPLALTFAPFLLLLFPTGRLPSRRWRP
                     LAWTAAISGTGVLLLNLLYKRPDTVGGAVTATTIVAVSLIFVTIFLSALSLVVRYHRA
                     SGVERQQLKWVALAAVLTIMFTIAQLSWLDQVLSDFVWTLLETAQQIVLYAAVGIAIL
                     TYRLYDIDVVINRALVYVSLTATLIAVYFGGIVLVQTTLRTLTAQESSLAVVASTLAI
                     AALFNPLRRRIQSFIDRRFYRRKYDARKTLEAFSARLREETDLQELNGELTRVVRETM
                     QPEHVSLWLRGSPRDAGRRT"
     gene            1328..2617
                     /locus_tag="VHM69_15225"
     CDS             1328..2617
                     /locus_tag="VHM69_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741793.1"
                     /translation="MSSSRTFPGGRRHFFQAAWVLVALVALAITAASAPADFERYGTL
                     CTEAPDSCLGRSQLTQEGLRELEGVGISLGLYAALGVGVSVLSKLVWFAVGALVFLLR
                     SRDRMALLVSCFLISFGTATLASESVDVLVTTHPAWWILGRGLQVLGEVLAVLFFFTF
                     PDGRFVPRWTPLLAAVFLAFQIPGDLFPGLYGMVPPVLNTLQTLVFMACVLGMIGSQV
                     YRYRNVSTFEQRRQTKWVVFGTTLAISLLIATMAPLFYLVLKVSETPSFIVLVIGDLI
                     PFIMLLIPLSIGIAMLRSGLFDIDLVINRALVYGTLTVSLVIVYLAAVVGAQRLLSPL
                     VGESNQLAVVVSTLAIAALFQPLRKRIQTFIDRRFYRNKYDASKLLAHFSSRLRDETD
                     LDRLGAELLSVVGKSMQPAHVSLWLRPDKSSINEQAD"
     gene            2804..3775
                     /gene="selD"
                     /locus_tag="VHM69_15230"
     CDS             2804..3775
                     /gene="selD"
                     /locus_tag="VHM69_15230"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX2741794.1"
                     /translation="MPPGDLEQVLEGISPTAVDGFVSGMETRDDAGFIPFGGVLLLQT
                     ADFFTPIVDDPYRFGQIAAANAFSDVYAMGGYPLTAMNLVAFPCSLDLGVLREILAGG
                     LSKIEESGAKLCGGHSVQDEEPKYGLSVTGFVKRDRVVRNAGAKPGDALVLTKPLGLG
                     ILTTALKRDLVSEEEIEDAVETASLLNGAARDAMNEIGVSAATDVTGYGFIGHLMEML
                     EASGVGAVVHRSQIPVWERAVPLANDGVYPGGLKSNREYLADRVTTDGVDQNDLLPLY
                     DPQTSGGLLVAVPEEQAEEFVNSLEKHGASGTLVGEVVEQSGVRVAR"
     gene            3873..4805
                     /locus_tag="VHM69_15235"
     CDS             3873..4805
                     /locus_tag="VHM69_15235"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX2741795.1"
                     /translation="MQSILNTALPFFALIFCGYAAGRLKLLDETAAAGVNTFVFYFAL
                     PAFLFGLMSSSPIGEVVNAPFIGAYVTASLTVFALAALVGRGLFKLLREEAAVLGLAA
                     VLANTGYMGIPLVSAVFGRESAVPVVVGLTIDGILMIPLGIMVIESSKGRAEGLFQTA
                     LTTLAALTRNPLILSIFAGLAVSAAGLTPPLPLTNFLDLLGDTAGPCALFALGATLAG
                     RPVAGGAAEVSYMTFMKLAVHPLALWFTMTRLFDVDPLWATAALLGAALPIAANVFIV
                     ARQYDVYVIRTSSAILVSTAISMISVSALLLVLH"
     gene            4991..5302
                     /locus_tag="VHM69_15240"
     CDS             4991..5302
                     /locus_tag="VHM69_15240"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX2741796.1"
                     /translation="MTSIMDTIEREVGGWPGVEEKEHRFGGIEFRVNGHEIGHLHGDR
                     LADLPFPVRMRKELVESGKAQLHHVLPQTGWVSYRIGGEEDVAGALDLFRKNYERLTR
                     V"
BASE COUNT          963 a         1744 c         1676 g         1093 t
ORIGIN      
        1 gggtagtgag ttctcaagag cttgccgtga acgactttgc tcgtaacggg tgccgtaacg
       61 tctgggagac ggacatcatg tagagtggtg tcgtgacaga agggacaagc gctcaaggcc
      121 gaggtgaaga ccccggcggg ataagcactc gaacggcctc atggctggca tggtcgctgt
      181 gcggcgcaac agtgttgatc ctggcgttgt cgctgttgct gatcctcctc ggctggtcga
      241 cgccgcagcc gcgcggggag gtaccgtggc ggaatcaggc catctccctg gttgggctcg
      301 taggtgcacc catcctcggc ggcctcatag catcgcgcct cccacgcaat ccctacggat
      361 gggtgtggct cggcttcgga ctgggcctca cccttcagca gctcgcagcg tcctacacag
      421 cctacgcgcg ggtggtggaa ccgggatcgc tcgtggctcc acgggccatc tcccacgtgc
      481 tggcgctggg agggcctctc gcgctcacct tcgccccctt ccttttgctg ctattcccga
      541 cgggtcggct tccgtcgcga cgctggcgtc ccctggcctg gaccgcggcc atttcgggga
      601 ccggggtcct gctcctcaat ctcctctaca aaaggccaga cacagtcggg ggagcggtca
      661 cggcaacgac aatagtcgct gtatccttga tcttcgtcac cattttcctc tcggcgctct
      721 cgctcgtcgt ccgctatcac agggcgagcg gggtggagcg ccagcagttg aagtgggtcg
      781 ctctggcagc cgtcctgacc attatgttca ccatcgcgca attgtcgtgg ctcgaccagg
      841 tgctcagtga cttcgtatgg accctgctcg agacagcgca gcaaatagtt ctctacgcag
      901 cagtaggaat cgccatactc acgtaccgcc tctacgatat agacgtcgtc ataaaccgcg
      961 ccctcgtcta tgtctcactg acggccacgc tcattgcagt ctacttcggc ggcattgtcc
     1021 tggtgcagac gacgctccgc actctgaccg cccaggagtc ctcgctggcc gtggtcgcct
     1081 caacccttgc gatcgccgcc ctcttcaatc ctctgaggcg gcgcatccag tccttcatcg
     1141 accgccgttt ttacaggcgc aagtacgacg ccagaaagac gctcgaagcc ttctccgcca
     1201 ggctcaggga ggagacggat ctgcaagagc tgaacggcga actgacgagg gtggtaaggg
     1261 agacgatgca gcccgagcat gtctcactgt ggctcagggg atcgccccgc gacgcaggga
     1321 ggagaacttg agttcgtcgc ggaccttccc gggcgggcgt agacacttct tccaggcagc
     1381 ctgggtgctg gtcgcattgg tggcgttggc gatcacggcc gcaagcgccc cggctgactt
     1441 cgagcggtac ggcaccctgt gcaccgaagc cccggatagc tgcctaggac gttcgcagct
     1501 cacgcaggag ggattgaggg agctggaggg ggtcgggatc tcgctcggac tctacgcggc
     1561 tctcggggtc ggggtctctg tgctctcgaa gctggtatgg ttcgccgtgg gcgcgctcgt
     1621 cttcctgctc cgctcccgtg accgcatggc gctgctggtc tcatgtttcc tgatctcatt
     1681 cgggaccgcg accctcgcca gtgaatctgt cgatgtcctt gtcacgaccc atcctgcatg
     1741 gtggattctg ggtcgcgggc tgcaggtttt gggagaagta ctcgcggtcc tcttcttttt
     1801 caccttccct gacgggaggt tcgtgccgcg ctggacgccc cttctcgccg cggtattcct
     1861 ggcctttcag atccccggcg acctcttccc cgggctgtac ggtatggtgc cgcccgtgtt
     1921 gaatacgctc cagacgctcg tattcatggc ctgcgttctc gggatgatcg ggtcacaggt
     1981 ctaccgctac cgcaacgtct ccacattcga gcaacgaagg cagacgaagt gggtcgtctt
     2041 cgggacgacg ctggcgatct cgctcctcat cgccaccatg gcgcccctct tctacctcgt
     2101 cctgaaggtg tcggagaccc cgtccttcat cgtgctggtg atcggggact tgatcccttt
     2161 tatcatgctg ctaatacccc tcagcatcgg catcgcgatg ctgagatccg gcctcttcga
     2221 tatcgaccta gttatcaacc gcgcacttgt ctacgggacg ctgacggtca gcctcgtcat
     2281 cgtgtacctc gccgccgttg tcggggcgca gcgcctgctc tcgccgctcg tcggagagag
     2341 caaccagctc gccgtcgttg tctccactct tgccatagcg gcgctgttcc aacctctgcg
     2401 aaagcgcatc cagacgttta tcgacaggcg tttctaccgc aacaagtacg acgcatcgaa
     2461 gttgctggcg catttcagct ccaggctgag agacgaaacg gacctggaca ggttgggagc
     2521 ggagttgctc tcggtggtcg ggaagtcgat gcagccggcc cacgtctctt tgtggttgcg
     2581 ccccgataag tcctccataa acgagcaggc ggattagcag cccttcgcgc tccgtggcga
     2641 agcggggtgg ataattgaag tgcgggcaag cccaccctct cgacgcccac ggactgcgct
     2701 caggcggaac ctgattccag cgcgggtata ctctcctgcg atggacgagc gtacacgcat
     2761 aaggctgacg aagtactcga cgaaggctgg ttgagcgtcc aagttgcctc caggggacct
     2821 ggagcaggta ctcgaaggga tctcaccgac cgcggttgac ggcttcgtga gcgggatgga
     2881 gacccgcgac gacgcggggt tcatcccgtt cggcggcgtc ctgctcctgc agacggcgga
     2941 tttcttcacg cccatagtgg acgatccgta ccgcttcggc cagatcgccg ccgccaacgc
     3001 cttctccgac gtttacgcga tgggagggta tccattgaca gcgatgaacc tcgtcgcatt
     3061 cccttgctcg ctggacctcg gggtcctgcg tgagatcctg gcaggaggcc tctccaagat
     3121 cgaagaatcc ggcgcgaaac tctgcggcgg tcactccgtc caggacgagg agccgaagta
     3181 cggcctctcc gtgacgggct tcgtgaaacg ggacagggtc gtgcgcaacg ccggagcaaa
     3241 gcccggagac gcgctggtcc ttacgaaacc cctcggcctc ggcatcctga cgaccgcgct
     3301 caagcgggat ctcgtctcgg aggaggagat cgaggacgcc gtcgagacgg cctccctcct
     3361 gaacggggca gcccgcgacg cgatgaacga gatcggtgtc tcagcggcaa cggacgtgac
     3421 cggctacggt tttatcggcc acttgatgga gatgctggag gccagcggcg tcggggccgt
     3481 cgtacaccgc agccagatcc cggtctggga gcgggccgtg ccgctcgcga acgacggcgt
     3541 ctacccaggc ggattgaaga gcaaccgcga gtatctggcg gaccgggtca cgacggacgg
     3601 cgttgaccag aacgatctac tcccgctcta cgacccgcag accagcggcg gtttgctcgt
     3661 ggccgtgcca gaggagcagg cggaggaatt cgtcaattcc ctagagaagc acggcgcttc
     3721 aggcacactc gtcggagagg tcgtagagca gtcaggcgtc agggtcgcca ggtaaccgcc
     3781 ggccctcagc cacgaggtct ggtaacgtaa gacccggtcc tcatcatcca ggaaagtcgt
     3841 cgtgaaactg atgcctgaag cctgaaacct cgatgcaatc cattctcaac acagcgctac
     3901 cttttttcgc cctcatcttc tgcggctacg ccgccggacg cctgaaactc ctcgacgaga
     3961 cggccgcggc aggagtgaat acgttcgtct tctacttcgc cctgcctgcg ttcttgttcg
     4021 gcctgatgtc ttcatcaccg atcggagagg tcgtgaacgc tccgtttatc ggggcttacg
     4081 tgacggcgag cctaaccgtc tttgcgcttg ccgcgctcgt aggacgcggg ctgttcaagc
     4141 tcctacgcga agaggccgcg gtcctcgggc tcgccgccgt gctcgccaac acgggctata
     4201 tggggatacc tctggtctcc gccgtattcg gccgcgaatc agcagtcccc gtagtcgtcg
     4261 ggctcaccat cgacggcata ctgatgatcc cactcggcat catggtcatc gagtcgtcca
     4321 aaggccgcgc agaaggcctc ttccaaacgg ctctgaccac cctcgcagca ctgacgcgaa
     4381 accccctgat cctctctatc ttcgccggac tggccgtctc cgccgccggc ctgactcccc
     4441 ccttgcccct gacgaacttc ctcgacctgc tcggcgacac ggcaggacca tgcgccctct
     4501 tcgccctggg cgcaaccctg gcaggacgcc cggtcgccgg cggcgcggcc gaggtctcat
     4561 atatgacgtt catgaagctg gcggtccacc cactcgccct gtggttcacg atgacccgac
     4621 tcttcgacgt agaccccctc tgggccaccg cggccctgct cggcgcagcc ctccctatag
     4681 ccgccaacgt gtttatcgtc gcccgccagt acgacgtcta cgtcatccgt acatccagcg
     4741 ccatcctggt ctccacagcc atatccatga tctccgtctc agccctcctg ctcgtcctcc
     4801 attagggacc cctccggaaa ccgtacgaag gtccaggtca gtacccaccc ttcgtccgtt
     4861 gcacatatta gtcgcccctc gcattatcag ggaatgtggg cccaccaatc ctacgcatct
     4921 tgacatcatc cacgtcggta gtaggcttta gttagccggc taattaacat tgatgagata
     4981 ggaggaaagc atgacgagca tcatggacac cattgagcga gaagtagggg gatggcctgg
     5041 ggttgaggag aaggagcaca ggttcggtgg gatcgagttc agggtgaacg ggcatgagat
     5101 cgggcacctg cacggcgaca ggttggccga ccttcccttc cctgtgagga tgaggaagga
     5161 gctggtcgag tcgggcaagg cgcagctcca tcacgtattg ccgcagacgg ggtgggtcag
     5221 ctaccgcatc gggggcgagg aggacgtggc tggggccctg gatctgttcc gcaagaatta
     5281 cgagcggctg accagggtat gagcgcgttc acggagaaag agatcgagta catcagagag
     5341 cggcgactgg gcaggctggc gacggtggat gctgagggcg ggccgcacgt cgtgcccgtc
     5401 gggttcaggt tcaacgagga gcttgacacc gtagacatcg gcgggcacga cctcggcgct
     5461 accaagaagt ttcggg
//
LOCUS       DATAIR010000643         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_342556, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000643 DATAIR010000000
VERSION     DATAIR010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342556"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VHM69_15245"
     CDS             <1..645
                     /locus_tag="VHM69_15245"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2741797.1"
                     /translation="AHLLEAAVDDIEYEDGRFFVKGSPDQAKTMDEVAFAAYTNVPEG
                     EEAGLEGVTYYDPPEMTYPFGAYICVVEIDRGTGQVEVKRFIAVDDCGVRINPTVVDG
                     QVHGGLAQGIGTALMEVIEFDEEGNHLNSSFMDYLIPTSMEVPNYELGEFVTPSTHHP
                     LGARGVAESPTVGSPPAVVNAVVDALSPYGVRNVDMPLTPAKVWQLLREHGVEA"
     gene            651..1610
                     /locus_tag="VHM69_15250"
     CDS             651..1610
                     /locus_tag="VHM69_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2741798.1"
                     /translation="MLPQDSNSLARRAESLRRAGELAGGERPFALITVVRTEPATSAR
                     VGDRAIVFADGTLEGWVGGGCIGPTARREALSALEEGMPRLVRVTPDTAATQTGVRMA
                     RMTCASEGTADLYVEPFLPRPTLIVAGDSPVAATLSAIAPPLGFRLLKMDEELTAGDV
                     PFPQDSWMVVATFGEFDEDAVEAGVRLGLPYVGLVASERRAGQVLSELRARGLEEGQL
                     SAVRSPAGLPLGTSGQEGISLSIMAEVSSLRAKLRPSFEPIRESLEQPGEAVDPVCGM
                     LVDVATARDVVEYGGTRYYFCGPGCKRAFEEEPEKYAAQVSAR"
     gene            1607..2203
                     /locus_tag="VHM69_15255"
     CDS             1607..2203
                     /locus_tag="VHM69_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2741799.1"
                     /translation="MSVVGVVMGAGRSSRFGSPKQLLPFGDTTLLGQVVKNANASSLD
                     RTIVVLGRASGELRETIDFGRAEVVENTAYGTGCASSLLAGLDAAGDDCEAIFLLLGD
                     QPGVLSEFVDRALSEWRGERPWAAVCSYMGRLGHPFVFSMEAFGDLRGLHGDKAVWKL
                     IEAYPDRVREIEIDAGLPPDVDTPEDYDRALAYSRNTS"
     gene            2206..2850
                     /locus_tag="VHM69_15260"
     CDS             2206..2850
                     /locus_tag="VHM69_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2741800.1"
                     /translation="MSSGVSAIVLAAGGGSRFGGGKLLAKLGGRPLIEAVLENLREAP
                     VDEIIVVVGTDAESLREVCDRYGARTIENEEWERGQSTSVLAGLRACGGGTRAAVVLL
                     GDQPFVGAEAVERLVAAFAEGAKIAVATYGGKRRNPVLFSRETWPLLEAELTGDEGAR
                     SVLRRHPDLVVEVPCYGVGDPADVDTREDLRRLEEMRGPVGGYNMVGRSKDGRS"
     gene            2893..3693
                     /locus_tag="VHM69_15265"
     CDS             2893..3693
                     /locus_tag="VHM69_15265"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2741801.1"
                     /translation="MKINNEFTVSAPIDQAWETMLNLERIAPCLPGAAIQEEKGEGEY
                     DGTMKVKIGPITANYKGTVKFDEVDEENHRAVLQATGRDARGQGTASATITSTLQEEG
                     DGTKVSVETDMKLTGRAAQFGRGIAQDVATKMLGQFASCLEEEIMGEPEEGAAAAAAE
                     PKGEENGSHDGTQPQAPPAAAAGGTAGRIISSEDPSLMAGGTVEGVVVAGAPEGAPTT
                     GQQPPPREEPEAFDIGAASQEAILKRVKPILVGAGVLFVLIWLFRRRR"
     gene            complement(3775..4230)
                     /locus_tag="VHM69_15270"
     CDS             complement(3775..4230)
                     /locus_tag="VHM69_15270"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX2741802.1"
                     /translation="MATHGTSLNRTALSATLHCLTGCSIGEVLGIVIGSALGWSNFAT
                     IVLAIVLAFFFGYLLTMLPLLRSGMALGAVLPLAFASDTLSITVMEIVDNLIIVVIPG
                     AMDAGLGSPLFWGSLAFALVIAGVAAFPVNRYLISRGKGHAVVHEHHGH"
     gene            complement(4346..5050)
                     /locus_tag="VHM69_15275"
     CDS             complement(4346..5050)
                     /locus_tag="VHM69_15275"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX2741803.1"
                     /translation="MHHYTKFGLPVLLLTLAILLASCGSAPGGSKQGAGTKDASTGQN
                     EKASRSPEPTTATSDMGDMGGMDHGSGGMATGMLMKNGRYSDERFIDAMVPHHQGAVE
                     MARVALKNAEHDEIKHLSRNIITTQKAEIKELKSIKQEVFGTSQVPMDLSMGEMKSMG
                     MMMNPGDLAKKDPFDKAFIDSMIPHHQSAIEMAQVALHESKNQRIKDLATNIVEAQKR
                     EISQMKQWREQWYPQG"
     gene            complement(5153..>5380)
                     /locus_tag="VHM69_15280"
     CDS             complement(5153..>5380)
                     /locus_tag="VHM69_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2741804.1"
                     /translation="IEGQVRGIQRMVDEEAYCVDILTQISSVVSASEKVATILLKDHV
                     EHCVRESIEKGDSADEKIEELTAAVERFLRV"
BASE COUNT         1009 a         1628 c         1768 g          975 t
ORIGIN      
        1 gcccatctgc tggaagccgc cgtggatgac atcgagtacg aggacgggcg cttcttcgtc
       61 aaggggtcgc cggatcaggc gaagaccatg gacgaggtcg ccttcgccgc atacaccaac
      121 gtacccgagg gcgaggaggc cggcctcgag ggcgtaacct actacgaccc gccggagatg
      181 acctacccgt tcggcgcgta catctgcgtg gttgagatag acaggggaac cgggcaggtc
      241 gaggtcaagc gctttatcgc tgttgacgac tgcggggtca ggatcaaccc gaccgtcgta
      301 gacgggcagg tgcacggcgg gctcgcgcag ggtatcggta ctgccctgat ggaggtcatc
      361 gagttcgacg aagagggcaa ccatctcaac agcagcttta tggactacct gatcccaacc
      421 tccatggagg tgcccaatta cgagctcggc gagttcgtga ccccatcgac gcaccatccg
      481 ctcggggcac gcggcgtggc agagtccccg acggtcggtt cgccccccgc cgtggtcaac
      541 gccgttgtgg acgcgctctc gccttacgga gtgaggaacg tggacatgcc tttgacaccc
      601 gccaaggtct ggcagctcct gcgcgagcat ggggtggagg cttaaaaacg atgctccctc
      661 aggactcgaa ctccctcgcc cgacgcgccg agtcgctgag gcgggcggga gagctcgcgg
      721 gcggggagcg tcccttcgcc ctgatcaccg tcgtccgcac cgagccggca acctcagcca
      781 gggtaggcga cagagctatc gtcttcgccg acggaacgct ggaaggttgg gtcggcggtg
      841 gatgcatcgg gccgacagcc cgtagggagg cgctctccgc gctcgaggaa ggcatgccgc
      901 gactcgtgcg cgtcacacca gataccgctg ccacccagac cggcgtccgc atggcccgca
      961 tgacctgcgc cagcgagggc accgcagacc tctacgtgga gcccttcctc ccgcgcccga
     1021 cgctcatcgt cgccggagac tcccccgtcg cagccacgct ctccgcgata gccccacctc
     1081 tgggattccg gctcctgaag atggatgagg aactgaccgc cggagacgtt cccttcccgc
     1141 aggactcatg gatggtggtc gccaccttcg gcgagttcga cgaggacgcc gttgaggcgg
     1201 gggtgaggct cggactgcca tacgtggggc tcgtcgcaag cgagcggcgc gccgggcagg
     1261 ttctctccga actgagagca cgagggctcg aagaaggaca gctctcggcg gtacgttccc
     1321 ctgccgggct gccgcttggc acgagcggtc aggagggcat ctcgctctcc atcatggcgg
     1381 aagtttcctc gctgcgcgcg aagctacgcc cgagcttcga accgatcagg gaatccctcg
     1441 aacaacccgg cgaagccgtt gaccctgtat gcgggatgtt ggtggatgtc gcgacggcca
     1501 gggacgtcgt cgagtacggc ggcacgcgct actacttctg cggccctgga tgcaaacggg
     1561 ctttcgagga ggagccggaa aagtacgcgg cgcaggtctc ggcccgatga gcgtagtcgg
     1621 cgtggttatg ggcgccggaa gatccagccg cttcggatct ccgaagcagc tcctcccgtt
     1681 cggggataca accctcctcg gccaggtcgt caagaacgcc aacgcctcgt ctctggacag
     1741 gaccatcgtc gtgcttggcc gcgcctccgg cgagctgcgc gagaccatcg acttcggacg
     1801 ggcagaggtt gttgagaaca ccgcctacgg tacgggctgc gcctcctcgt tgcttgccgg
     1861 cctcgacgct gccggggacg actgcgaagc catcttctta ctcctcggcg accagcccgg
     1921 cgtactgtcc gagttcgtag accgggcgct ctcggagtgg agaggcgaaa ggccgtgggc
     1981 ggcggtctgc tcgtacatgg gcaggctcgg tcatcccttc gtcttctcca tggaagcttt
     2041 tggggatctc agaggtctgc acggagacaa agccgtctgg aagctcatcg aggcatatcc
     2101 tgacagggtt cgagagatcg agatcgacgc agggttgccg ccggacgttg ataccccgga
     2161 ggactacgat cgggctcttg cctacagccg gaatacctct tagccgtgtc gtccggcgtt
     2221 tccgcgatag tgctcgccgc cgggggaggg agccggttcg gaggaggtaa gctgctcgcg
     2281 aaactcggag gtcggccgct catagaagcc gtcctcgaaa acctgcgcga agcacccgta
     2341 gacgagatca tcgtggtcgt cggcacggac gcggagagtc tacgcgaggt ctgcgatcgg
     2401 tacggtgcaa gaactatcga gaatgaagag tgggagcggg gccagtcaac gtcggtcctc
     2461 gccggactga gggcgtgtgg tggagggaca cgagccgcgg tagtcttgct cggggaccag
     2521 ccgttcgtcg gggcggaggc agtggagcgg ctcgtcgcgg ctttcgctga aggggcgaag
     2581 atcgcggtgg cgacgtacgg agggaaacgg cgcaacccgg tgctgttttc cagggagaca
     2641 tggccgctgc tcgaagcgga gttgaccgga gatgaggggg cgaggtctgt gttgcggcgg
     2701 catcctgatc tggtggtcga ggtgccttgc tatggggtgg gggacccggc tgacgtggac
     2761 accagggagg acctgaggcg tctggaggag atgcgcgggc cggtgggcgg ttataacatg
     2821 gttgggcgat ccaaagacgg tcgcagctga taatcctgca atcgcgactc cttattggaa
     2881 ggaggaggtt ccatgaagat caacaacgag ttcacggtga gcgctccgat cgatcaggcg
     2941 tgggagacca tgttgaacct ggagaggatc gcgccatgcc tcccgggggc ggccatccag
     3001 gaggagaagg gcgaaggcga gtacgacggc acgatgaagg tgaagatcgg cccgatcacg
     3061 gccaactaca agggtaccgt gaagttcgac gaggtcgacg aggagaacca tcgcgctgtc
     3121 ctccaggcaa cgggccgcga cgcccgcggc cagggtactg cctccgcgac catcacatcg
     3181 acgttgcaag aggaaggcga cggcaccaag gtcagcgtcg agacggacat gaagctcacg
     3241 ggacgcgcgg cccagttcgg gcgcggcatc gcccaggacg tggccacgaa gatgctcggc
     3301 cagttcgctt cctgcctgga agaggagatc atgggcgaac ccgaagaggg agccgccgca
     3361 gcagccgcgg aacccaaagg cgaggagaat ggctcacacg acggcacgca gccacaggct
     3421 cctcccgccg ccgcagcagg cggcacggca ggcaggatca tcagcagcga agacccgagc
     3481 ctcatggccg gaggcacggt cgaaggagtc gtcgtcgccg gggcgccgga gggcgcaccg
     3541 accaccggac agcagccacc accccgcgag gaaccggaag ccttcgacat cggtgcggcg
     3601 agccaggagg ccatcctcaa gcgggtaaag cccatcctcg tcggcgcagg cgtgctcttc
     3661 gtcctgatct ggctcttccg ccgcaggcgc taggcattca gcaagatcaa aagctaaggc
     3721 tgagacctga ctgctgaagg cacgggttga ggaagttttc ttcctcaacc cgcttcaatg
     3781 accgtggtgc tcgtggacga cggcgtgacc tttgccgcgt gagatcaggt acctgttgac
     3841 agggaaagcc gcgacacccg ctataaccag cgcgaacgcg aggcttcccc agaacagagg
     3901 gctgccgagc cccgcgtcca tcgcgccggg gatcaccacg atgatgaggt tgtcaactat
     3961 ctccatgacc gtgatcgaaa gcgtatcgga ggcgaaggca agcggcaata ccgcaccgag
     4021 cgccatgccc gaccgcaaga gcggcagcat cgtcaacagg tagccgaaga agaaggcaag
     4081 cactatcgcg agcacgatgg ttgcgaagtt gctccatccg agcgcgctac cgatcacaat
     4141 gccgagcacc tcaccgatgg aacatcccgt cagacaatgc aacgtcgcac ttaaggccgt
     4201 ccggttgaga gaagtgccgt gggttgccat gcaactcctt cctatgcctg tctaccaact
     4261 aaactatata ccccctgggg gtatttgtaa agaattggat tgcgcggcgg aaaggtaggc
     4321 gcgcaatcct gatgagggcg ggcttctagc cttgcgggta ccactgttcg cgccattgtt
     4381 tcatctgaga gatctcacgc ttctgcgctt cgacgatgtt ggttgcaagg tcctttatcc
     4441 tctggttctt gctctcgtgc agggcgacct gggccatctc gatagcggac tggtggtgag
     4501 ggatcatgct gtcgatgaac gctttgtcga aggggtcctt ctttgccagg tccccgggat
     4561 tcatcatcat acccatactt ttcatctcgc ccatgctcag gtccatgggg acctgggagg
     4621 taccgaacac ctcctgtttg atcgacttga gctctttgat ctctgccttc tgggtggtga
     4681 tgatgtttct ggagaggtgc tttatctcat cgtgctcggc gttcttcagc gcgaccctgg
     4741 ccatctccac tgctccctgg tgatggggga ccattgcgtc gataaagcgt tcgtccgagt
     4801 atctgccgtt cttcatcaac atcccggtag ccatgccgcc ggagccgtga tccataccgc
     4861 ccatgtcgcc catatcggac gtggcagtag tgggctccgg ggaccgtgag gctttctcgt
     4921 tctggccggt ggaggcatct ttcgttccgg ctccctgttt ggatcctcca ggagcgctgc
     4981 cgcaggaggc cagcaggatg gcgagggtga gcaggaggac cggcagaccg aacttcgtgt
     5041 aatgatgcaa gataggcccc tttccgtttt gaaactcgtc tgtgatccaa cgccgctctc
     5101 gtcgtggcgc agctcatttc ctccacgttg gaggattccc ccttgcgtac ttctagactc
     5161 gtaggaaccg ctcgacggcg gcggtaagct cctctatctt ctcgtccgcg ctatcgccct
     5221 tctcgatgga ctcgcgcacg cagtgctcga cgtggtcctt gagcaggatg gtcgccacct
     5281 tctccgaggc tgagacgacg ctgctgatct gggtgaggat gtccacgcag tacgcttctt
     5341 cgtccaccat gcgctgtatc ccgcgcacct gcccctctat
//
LOCUS       DATAIR010000644         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_44455, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000644 DATAIR010000000
VERSION     DATAIR010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44455"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1175
                     /locus_tag="VHM69_15285"
     CDS             114..1175
                     /locus_tag="VHM69_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX2741805.1"
                     /translation="MVSTTTGSRMMDSTDKEILNRIQREVPLEREPFEAIGREMGIAG
                     DEVIRRIEALKRGRVIRQISAIFDTRVLGYESSLVAATIPAHKLNEGAKAINSHPGVS
                     HNYERNNEFNLWYTVAVPPDSRLGLEGTVDVLHHISGAEKTRILPTLKLFKIGVTLDM
                     NTGATAKKEAPQYGESDRASADKNISEDDKAAIRALQEDVPLTPRPFDLWGRQVGLSY
                     EELLERAFDLRDRKIMRRFSAVLYHRKAGFRANAMGVWRVPDERIDEVGNAFAHFQAV
                     SHCYQRPTYEDWPYSVFSMVHGRTVEECENVLSAMAEETGLTEYTSLYSTREYKKTRV
                     RYFTPEMAAWERLYAGILR"
     gene            1217..1954
                     /locus_tag="VHM69_15290"
     CDS             1217..1954
                     /locus_tag="VHM69_15290"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2741806.1"
                     /translation="MSAALIVALFAALLAGIVTGLTGFGLALISTPILLFVYEPRTVI
                     VLTAMFSIFISSAVVWDSWHEARRRLALALLIPALVGIVAGAEVLRVIDPDYVRLGVG
                     VIVIFSALLLVRDIRLPGAGTRLGTLVAGSASGALSTSTGLAGPPIVLLLASRNLPKH
                     EFRGTSALYFLPMSIVGLAVLSLRGLVEPGEILLGLLLIPAAIAGKAVGTALLGRVSE
                     KKFRAITLGLVILTGTLGVATALWALL"
     gene            2118..2906
                     /locus_tag="VHM69_15295"
     CDS             2118..2906
                     /locus_tag="VHM69_15295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2741807.1"
                     /translation="MTVRVGAQLHPQQASYAQMRDAWLRVEEMGADTLFNWDHFFPLY
                     GDPDGKHLECWTLLGAMAEVTERVEFGALVTCNSYRNANLLADMARTVDNISGGRLIL
                     GIGSGWFQRDYDEYGYEFGTAGGRLRDLDAAMPVIEERLGKLNPPPTREIPILIGGGG
                     EKVTLRIVAEHAHIWNGFGNPEEAGRKSRILDDWCRKVGRDPKEIERSIMVRPNQVQN
                     AARYVENGITHLLMGFGGPDYDVSPLQDLIAWRDEYRRRNENAG"
     gene            complement(3018..>3416)
                     /locus_tag="VHM69_15300"
     CDS             complement(3018..>3416)
                     /locus_tag="VHM69_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2741808.1"
                     /translation="MRQHEENAIAVAEFLQGHPEVETVLYPGLPAHPQHDLAKRQMSG
                     FSGMVSFTLKGGTEAAYAAVQKTRIFHFAESLGGVESLITHPATMTHAAIPREQREAR
                     GVTDGLMRLSVGIEDKEDLISDLDQAISPD"
BASE COUNT          654 a         1075 c         1085 g          602 t
ORIGIN      
        1 tcggcctgaa cggaacaacg taaacaatct catacccagt tttggccgcg cctgttatag
       61 ttgcggtctt agtagtaaaa cgtaggaaaa ccgtcgagaa gggctcgtaa agcgtggtaa
      121 gcacaactac cggcagtcgc atgatggata gcaccgacaa ggagatactc aaccggatcc
      181 agcgcgaggt cccgctggag cgggagcctt tcgaggccat cgggcgcgag atggggatcg
      241 cgggcgacga ggtcatccgg cgcatagagg cgctcaagag ggggcgtgtg atccgccaga
      301 tcagcgcgat cttcgatacg cgcgtcctcg gctacgagtc gagcctggtc gcagccacga
      361 taccggccca caaactcaac gagggcgcga aggcgatcaa ctcccacccc ggcgtctccc
      421 acaactacga gcggaataac gagttcaacc tctggtacac cgtcgccgtc cctcccgact
      481 cccgcctggg actggaaggc acggtggacg tgctgcacca cataagcggc gcggagaaga
      541 ccagaatctt accgacgctc aagctcttca agatcggtgt gaccctggac atgaacacgg
      601 gcgccaccgc gaagaaggag gccccgcagt acggggagtc cgaccgcgcg agcgcagaca
      661 aaaacatctc ggaggacgac aaggcagcca tcagggcgct ccaggaggac gtcccgctga
      721 cgccgcgccc gttcgacctg tggggccgtc aggtcgggct ctcctatgag gagctactgg
      781 agagggcctt cgacctgcgc gaccgcaaga tcatgcgccg cttctcggcg gtcctatacc
      841 accgcaaggc cggcttcagg gccaacgcga tgggggtctg gagggtgccc gacgaacgca
      901 tcgacgaggt gggcaatgct ttcgcccact tccaggccgt atcccactgc taccagcgtc
      961 caacgtacga ggactggcca tactccgtct tctccatggt ccacggccgc accgtcgagg
     1021 agtgcgagaa cgtcctttcg gccatggccg aggagacggg cctgacggaa tacacctccc
     1081 tctactcaac acgcgagtac aagaagacgc gggtgcggta cttcaccccc gagatggctg
     1141 cctgggagcg cctctacgcg ggcatcctgc gctgaaatag gtctcaggct tcaggtatta
     1201 ggggaacgct agcttgttga gcgcggcgct gatcgtggca ctcttcgcgg cattgctcgc
     1261 ggggatcgtt acgggcctga cgggcttcgg gctcgccctg atcagcaccc ccatactcct
     1321 cttcgtctac gagccgagga cggtaatcgt actgacggcg atgttctcga tctttatcag
     1381 cagcgcggtt gtgtgggact cgtggcacga ggcgcggcgg cgtctcgcgc tcgcgctctt
     1441 gatacccgcc ctcgtcggca tcgtggccgg tgccgaggtc ctgcgcgtga tagacccaga
     1501 ctacgtgcgg ctcggggttg gcgtcatcgt gatcttctcc gccctgctcc tcgtccgcga
     1561 catccggttg cctggagcgg gtacccgttt ggggacgctc gtcgcgggct cggcgagcgg
     1621 ggcgctctcc acctcgaccg gactggccgg tcccccgata gtgctgctcc tggcttcgcg
     1681 caatctcccg aagcacgagt tcagggggac cagcgccctg tacttcctgc cgatgagcat
     1741 cgtcgggctg gccgtacttt cactgcgcgg cctcgtcgag ccaggagaaa tactgctcgg
     1801 gctcctgctc atccccgccg ccatcgcagg aaaggccgtg ggtacggcgc tccttgggcg
     1861 cgtctccgag aagaagttcc gtgcgattac ccttgggctc gtgatcctga ccggcaccct
     1921 cggcgtcgcg accgcgctgt gggcgctgct gtagcaagaa ctggtcgaag agcgcaacca
     1981 tcgcaggtat gagaccgccg accatgcgaa aacctgccgt tctttgagac tctagctgct
     2041 accggtctgg cctgttacct tcctgatgct acccgcgtgg cataatctcc cagttggcca
     2101 ggtacaggag gcgaaggatg acggtaaggg ttggggctca gcttcatccg caacaagcca
     2161 gttacgcgca gatgagggac gcgtggctca gggtagagga gatgggggct gacacgctct
     2221 ttaactggga tcacttcttc ccgctctacg gcgatcctga cggcaagcac ctcgaatgct
     2281 ggacgctcct cggggcgatg gccgaggtta cggagagggt ggagttcggg gcgctcgtca
     2341 cgtgcaactc ctaccgaaat gcgaacctgc tcgcggatat ggcccgcacc gtggacaaca
     2401 tctccggcgg acgtctcata ctggggatcg gctccggctg gttccagagg gactacgacg
     2461 aatacggcta cgagttcggg acggcggggg gtcggctacg ggacctcgac gccgcgatgc
     2521 ccgtcatcga ggagcggctc gggaagctca acccgccacc cacgcgtgag atcccgatcc
     2581 tcatcggggg cggaggggaa aaagtcaccc tacgcatcgt ggccgagcac gcccacatct
     2641 ggaacggctt cgggaaccct gaggaggcgg gccgcaagag ccgcatcctc gacgactggt
     2701 gccgcaaagt cgggcgcgac ccgaaagaga tagagcgctc gataatggtt agaccgaacc
     2761 aggttcaaaa cgcagcccgc tacgtggaga acggcatcac gcacctactc atgggcttcg
     2821 gcggccccga ctacgacgtg agccccctgc aagacctcat cgcatggcgc gacgaatatc
     2881 ggcggcgtaa cgaaaatgcc ggttgaccaa ctggcggaaa gtgtgactcc cccagaacaa
     2941 tcaggcttgg tcgagaaccg gttcgaggac agactcggct acccgtacgt cttcgccccg
     3001 taaagggacc actcctatca gtcagggctt atcgcctggt ccaggtcgga gatcaaatcc
     3061 tccttgtcct cgataccgac agagagacgc atgaggccgt cagtgacgcc cctcgcctcg
     3121 cgttgctcgc gcgggatggc ggcgtgggtc atggtggcgg gatgggttat gagcgactcg
     3181 accccgccca ggctctctgc gaagtggaag attcgcgtct tctgcaccgc cgcataagcc
     3241 gcctcggtgc cacccttgag ggtgaacgag accatccccg agaagccgct catctgcctc
     3301 ttcgccagat cgtgctgggg gtgtgctggg aggccagggt ataacaccgt ctccacctcg
     3361 gggtggcctt gcaagaactc ggcgacggcg atggcgttct cctcgtgctg gcgcat
//
LOCUS       DATAIR010000645         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_137416, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000645 DATAIR010000000
VERSION     DATAIR010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137416"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VHM69_15305"
     CDS             <1..1054
                     /locus_tag="VHM69_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2741809.1"
                     /translation="SWSLGNLNLDLPCLASALDAAVDPTTAGTIGSLGGLAVLNLEGL
                     QTRYEDPGPVFEEIASLPEHKATRIMQEIYAEPIREELIFRRVQEIKDKGVIAAASLT
                     PQRVERYHRAAIEAGLDVLVVQGTVVSAEHVSRSVEPLNLMEFVPSLNVPVIVGGCAS
                     YSTALHLMRTGAVGVLVGVGPGRICTTRGVIGVGVPQATAVADAAAARMRHYLETGEY
                     VNVIADGGMRTGGEIAKAITCGADAVMLGSAFARAEEAPGRGYSWGMATFHPTLPRGT
                     RISTRTTGTLEEILVGPARENDGTLNLMGALRTSMATTGYQNIKEFQKAEVMYAPSLA
                     TEGKTEQRSQGVGMGK"
     gene            1055..2575
                     /gene="guaA"
                     /locus_tag="VHM69_15310"
     CDS             1055..2575
                     /gene="guaA"
                     /locus_tag="VHM69_15310"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2741810.1"
                     /translation="MILVLDFGGQYSQLIARRVREARVYSELIPYDTPVDEIERRNPD
                     GIILSGGPSSVYEEGAPRVDDMLFDLDVPILGICYGMQLMALVEGGDVGAVQIREYGR
                     SDLRVKSHDGLFAGTPDQQKAWTSHGDAVFSVPEGFEVTAETPSVPIIAFEDRERGRH
                     GVQFHPEVSHTEYGMEILKNFLYEACGCKPEWTPVNIITDAVERIREQVGDARAICGL
                     SGGVDSATAAMLVHRAIGDNLTCVFVDHGLLRHNEAEQVVEAFGENSDVPLIHVDASG
                     RFLDKLANVTDPEAKRKIIGEEFIRTFEKEAGRLEDAKFLVQGTLYSDVIESGTRDAA
                     KIKSHHNVGGLPEVMDLDLVEPLRNLFKDEVRVVAAELGMPERMVWRQPFPGPGLAIR
                     VIGDVTAERLEILRKADAVLQDEIRNAGLYRELWQSFAVLPAIQSVGVMGDARTYAYP
                     VVIRAVTSDDAMTADWARLPYDLLEKISNRIINEVPGVNRVALDVTSKPPGTIEWE"
     gene            2876..3916
                     /locus_tag="VHM69_15315"
     CDS             2876..3916
                     /locus_tag="VHM69_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2741811.1"
                     /translation="MSRIVRSRLLIVMSVVALSVVLAACGGSAGGESGGGGGGEVSGT
                     INIEGSSTVQPITQAAAELFREEYPDAKIEVGGAGTSDGFEAFCQGDTQISDASRPID
                     VAEEVPVCKENGVEFIEIPVAYDGISVVVNKKGNDWATDVTSEELKTMWEPAAEGKIT
                     KWSQVRSSWPDRELSLYGPGTESGTYEFFNETIVGNEEEVNRSDYEASEDDNVLVQGV
                     SGDENALGYFGYSYYENNLDTLRALAVDGIKPTEDSIRSGEYVLSRPLFIYVSTDALK
                     NNKAVKPFVDFYVSEQNLDRLVEAAKYVTLPESLSQESRTQYEDRTTGTVFTEAGEPK
                     GGDLETALKQSQ"
     gene            4012..>4592
                     /locus_tag="VHM69_15320"
     CDS             4012..>4592
                     /locus_tag="VHM69_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741812.1"
                     /translation="MAIEAGGTRSGAGAWKRDSRTRELRERVIKLLLTACAYLSIFTT
                     VGIVVVLFEETARFFLEVSPVEFLTSTRWSPQQGSFGVLPLVYGTLMATFIAIIVALP
                     IGLLTAIYLSEYAPQQLRRWLKPMLEILAGVPTVVYGYFALTFITPFFRDSVFPGIGS
                     FNALAAGLIMGVMIIPTVASLSEDAMSAVPSELR"
BASE COUNT          891 a         1419 c         1486 g          796 t
ORIGIN      
        1 ctcctggtcg ctgggcaacc tgaacctcga cctgccatgc ctggcaagcg cgctcgacgc
       61 cgccgtggac ccgacgacgg caggcactat cggttctctg ggcggcctcg ccgtcttgaa
      121 cctcgaaggg ttgcagacca ggtacgagga ccctggtccc gtcttcgagg agatagccag
      181 cttgcccgag cacaaggcca cacggatcat gcaggagatc tacgccgagc cgattagaga
      241 agagctgatc ttccgccgtg tgcaggagat taaggacaag ggagtcattg cggcggcctc
      301 gctcaccccc cagcgcgtcg agcgctacca tagggccgcc atagaagcgg gcctggacgt
      361 cctcgttgtg caggggacgg tggtctccgc cgaacacgtc tcgagatcgg tcgaaccgtt
      421 gaacctgatg gagttcgtgc cgtccctgaa cgtccccgtc atagtgggcg gctgcgcgag
      481 ctactcgacg gcgctccacc tcatgcggac gggtgccgtc ggtgtactcg tcggtgtcgg
      541 gcccgggcgt atctgcacca cgcggggtgt gatcggggtc ggcgtgccgc aggcgaccgc
      601 cgtcgcagac gccgccgccg cccgtatgcg ccactacctg gagaccggcg agtacgtcaa
      661 cgtcatcgcc gacggcggga tgcgcacggg aggtgagatc gccaaggcca taacctgcgg
      721 tgccgatgcc gtcatgctcg gcagcgcttt cgcgagggcc gaggaggcgc ctggccgtgg
      781 ctactcgtgg gggatggcta ccttccaccc gacgctccct cgcggcaccc gcatcagcac
      841 gaggactacg gggacgctcg aggagatact cgttggaccc gcccgcgaga acgacgggac
      901 gcttaacctc atgggcgccc tgaggacgag catggcgacg acgggctacc agaacataaa
      961 ggagttccag aaggccgagg tcatgtacgc gccctcgctc gcgaccgagg gcaagacgga
     1021 gcagcgctcc cagggcgtcg ggatgggaaa gtagttgatc ctcgtcctcg acttcggcgg
     1081 gcagtactcc caactcatcg cccgccgggt acgtgaagcc cgcgtctatt ccgagctgat
     1141 cccctacgat acccccgtcg atgagatcga gcgtcgcaac cccgatggga tcatcctctc
     1201 cggcggcccg agcagcgtct acgaggaggg cgcgccgcgg gtggacgaca tgctcttcga
     1261 cctcgacgtg cctattttgg gcatctgcta cgggatgcag ctcatggcgc tcgttgaggg
     1321 aggggacgtc ggggccgtcc agatacggga gtacggacgt tctgaccttc gcgtcaagag
     1381 ccacgacggc ctcttcgcag gcacgccgga ccagcagaag gcctggacga gccatggcga
     1441 cgccgtattc tccgtccccg agggattcga ggtcacggcc gagacgcctt ccgtccccat
     1501 aatcgccttc gaggaccggg aacgtggccg ccacggggtc cagttccatc ccgaggtcag
     1561 ccacaccgag tacggcatgg agatcctcaa gaacttcctc tacgaggcgt gcggctgcaa
     1621 gcccgagtgg accccggtca acatcatcac cgacgccgtc gagaggataa gggagcaggt
     1681 cggcgacgcc agggccatct gcgggctctc cggcggggtc gactcggcga cagcggcgat
     1741 gctcgtccac cgcgccatcg gcgacaacct gacgtgcgtc ttcgtcgacc atggactcct
     1801 caggcacaac gaggccgagc aggtcgtcga ggctttcggg gagaactccg acgtgccgct
     1861 gatccatgtt gacgcgtctg gacgttttct cgacaagctc gccaacgtca ccgacccaga
     1921 ggccaagcgc aagatcatcg gcgaggagtt catacgcacc ttcgagaagg aggctggcag
     1981 gctggaggat gccaagttcc tcgtacaggg cacgctgtac tcggacgtaa tagagagcgg
     2041 aacccgcgac gccgcaaaga taaagagcca ccacaacgtc ggcggcctgc ccgaggtcat
     2101 ggacctcgac ctcgtcgagc ccctgaggaa cctcttcaaa gacgaggtgc gcgtcgtcgc
     2161 tgccgagctg gggatgcctg agaggatggt ctggaggcag cccttccctg gaccagggct
     2221 cgccatcagg gtcataggcg acgtcacggc cgagaggctg gagatcctcc gcaaagccga
     2281 cgcggtgttg caggatgaga taagaaacgc agggctctac cgcgagctgt ggcagagctt
     2341 cgcagtgttg cccgcgatcc agtccgtcgg cgtcatgggc gacgcccgta cttatgctta
     2401 ccccgtcgtc atcagggccg tcacctcaga cgacgcgatg accgcggact gggccagact
     2461 cccctatgac ctgctggaga agatcagcaa ccgcatcatc aacgaggtgc ctggcgtaaa
     2521 ccgcgtcgcc ctggatgtca caagcaagcc cccaggcaca atagaatggg aatgagtagg
     2581 catcaggtca caggcatcag gagagcgacc cggttcggga cgccttcgcg taaggtgtgg
     2641 cgcatacgac tactcggtga aagtcgaacc ggatctcgtc ccagcagccg aggcctgaaa
     2701 cctgaagcct atttaaccta gatttagcgt ggagtttaca taactgcaac taaatcccgg
     2761 gagttatgcc ctatcatccc gccacggaag tcgagtgaga ggttcccaag ttattcggtc
     2821 gaggatcttg cactcgaggt acgtgaatcg taatggagcg aagggagcgt aggtattgag
     2881 taggatcgta cgttcgaggc tgttgatagt gatgtccgtt gtggctctgt cggtcgttct
     2941 ggccgcctgt ggcggcagcg ctggcggcga aagtggtggc ggcggtggcg gtgaggtctc
     3001 cggtaccatc aacatcgagg gctccagcac cgtgcagccg atcacgcagg ccgctgccga
     3061 gctattccgg gaggagtacc cggatgcgaa gatcgaggtc ggcggcgcgg ggaccagcga
     3121 cggcttcgag gcgttctgcc agggcgacac ccagatctcc gacgcttcca ggccgataga
     3181 cgtggccgaa gaagttcctg tttgcaagga gaacggggtc gagttcatag agatcccggt
     3241 cgcctacgac ggcatctcgg tcgtcgtcaa caagaagggc aacgactggg cgaccgacgt
     3301 cacgagcgag gagctcaaga cgatgtggga gcctgccgcg gagggcaaga tcacgaagtg
     3361 gagccaggtg cgctccagct ggccggatag ggaactgagc ctctacggtc ctggcaccga
     3421 gtccggcacc tacgagttct tcaacgagac catcgttgga aatgaagagg aggtcaaccg
     3481 ctcggactac gaggccagcg aggacgataa cgtgctcgtg cagggtgtct ccggtgacga
     3541 gaacgcgctc ggttacttcg ggtactcgta ctacgagaac aacctcgaca ccctcagggc
     3601 gctcgccgtc gacggcataa agcccaccga ggattccatc cgctccggcg aatatgtgct
     3661 ctcccgcccg ctgttcatct acgtgagcac cgacgcgctc aagaacaaca aggcggtcaa
     3721 gcccttcgtt gacttctacg tctcggaaca gaacctcgac cggctcgtcg aggccgcgaa
     3781 gtacgtgacg ctgccggaga gtctgtcgca ggagtcgcgg acccagtatg aggaccgcac
     3841 cacaggcacc gtgttcaccg aagcaggcga gcccaagggc ggcgacctgg agacggcgct
     3901 caagcagtcg cagtaaccgt ttgtaccggg acgggtctgc ttgtaacata tgggggccgg
     3961 cacaacagcc ggtccccatc cttatgatca cgaggtttcg gaggattttc ggtggcgatt
     4021 gaagcggggg gtacgcggtc aggggcgggt gcctggaagc gcgacagccg caccagggag
     4081 ctaagggagc gggtcataaa gctcctgctc acggcatgcg cgtacctttc aatcttcacg
     4141 acggtcggta tcgtcgtcgt cctcttcgag gagaccgccc ggttcttctt ggaggtctct
     4201 cccgttgagt tcctgacttc gacgcgctgg tcgccgcaac agggcagctt cggcgttctg
     4261 cctctcgtct acggcacgtt gatggcgacg tttatagcca taatcgtagc gctcccgatc
     4321 gggctgctca cggccatcta cctcagcgag tacgccccgc agcagttgcg gcgatggcta
     4381 aagccgatgc tcgagatcct ggctggcgta cccaccgtgg tctacgggta cttcgccctg
     4441 acgttcatca ccccgttctt cagggacagc gtcttcccgg gcataggctc cttcaacgcg
     4501 ctcgccgcgg gcctcatcat gggcgtgatg atcatcccca cggttgcctc gctctcggag
     4561 gacgcgatgt ccgccgttcc gagcgagctg cg
//
LOCUS       DATAIR010000646         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_414226, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000646 DATAIR010000000
VERSION     DATAIR010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414226"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VHM69_15325"
     CDS             complement(<1..435)
                     /locus_tag="VHM69_15325"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741813.1"
                     /translation="MVRSAGRTAARAIRRPSAARVSLLIPTHALPPLSYRIPERLQGE
                     IRVGATVVAPLSGRHRLGVVVGTERASEQAREDVLCAVPDLSLRPEIVELCLRIGEYA
                     AVPLPTALRAALSPGIDAGRYRVLKPSPDWPWERNKLVTRTAL"
     gene            complement(436..1674)
                     /gene="metK"
                     /locus_tag="VHM69_15330"
     CDS             complement(436..1674)
                     /gene="metK"
                     /locus_tag="VHM69_15330"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2741814.1"
                     /translation="MVERTAYTTTWNAADDQKLKTSHLFTSESVTEGHPDKIADQISD
                     TILDAALADDPMSRVACETLVTTGLVVVAGEITTSTYVDIPTLVREKVARIGYTRAKF
                     GFDAQTCGVIVSIDPQSADIAQGVDRALEVRDGDQDLDEIGAGDQGMMFGYACEETEE
                     LMPLPITLAHALTRRLSGVRKSGDLDYLRPDGKSQVTVRYEGDKPIGIEKVLISAQHR
                     DGAEAHIRDDLVETVIRPVMPEDLFDESVAEILVNPTGRFVTGGPQGDTGLTGRKIIV
                     DTYGGSAPHGGGAFSGKDPTKVDRSASYAARWVAKNLVAAGLARRCMVQVAYAIGVAH
                     PVSVMVETFGTARMDPAALGALVQEHFDLRPGAIIRDLDLRNPIFAQTAAYGHFGRRE
                     PGFTWEETSRADDLRRAAGF"
     gene            complement(1677..>2474)
                     /locus_tag="VHM69_15335"
     CDS             complement(1677..>2474)
                     /locus_tag="VHM69_15335"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX2741815.1"
                     /translation="DPGTAGHPAVRENLRLLRKDGCRIIEGHDGGMAPAGEVVAGLLG
                     SLGGPMSGRRLLITAGGTREPIDSVRFIGNRSSGKMGLSVAREALRLGADVTVVAANI
                     ESVEPGTETLPVESVEDLQEAVLERAGNAHALVMAAAVSDFTPASPVKDKIRRGTGTT
                     SLDLAPTSDILKAVRERYPDLFVVGFAATHGDPVTDAREKLASKGADLVVGNDISREG
                     VGFGADENEVYVVGPEGERFVPRASKEEVARVILDSMIVEMDKERQS"
BASE COUNT          390 a          822 c          781 g          481 t
ORIGIN      
        1 cagtgcggtg cgcgtaacga gcttgtttct ctcccatggc cagtccgggg atggtttcaa
       61 gaccctgtaa cgtccggcgt cgatgccggg cgagagggcg gctctcagcg cggtcggcaa
      121 aggaacggcg gcgtactcgc cgatcctgag gcagagttcc acgatttcag gtctcaggga
      181 gagatcaggt acggcgcaaa gtacgtcctc gcgcgcttgc tcgctcgccc tttcggttcc
      241 gactacgaca cccagacgat gccttcctga gagtggcgcg acgaccgtgg cgccgaccct
      301 gatttcgccc tgcagacgct ccggtatcct gtagctaagc ggcgggaggg cgtgcgttgg
      361 aatgagcagg cttacccgtg ctgcggaagg ccgtcggatg gcgcgggcgg ccgtacggcc
      421 tgcactcctt accaactaga agccggcggc gcggcggaga tcgtcggcgc gcgaggtttc
      481 ctcccaggtg aatcccggct cgcggcggcc gaaatgaccg taagcggcgg tctgcgcgaa
      541 gatagggttc ctcaggtcca ggtcgcggat gatggcgcct ggcctgaggt cgaagtgctc
      601 ctgaaccagc gcgccgaggg ccgcggggtc catcctggcc gttccgaaag tctcgaccat
      661 cacgctgacg gggtgggcga cgccgatggc gtaggccacc tggaccatgc accgtctggc
      721 gaggccggcg gcgacgaggt tcttcgcgac ccatctggcc gcgtaggagg ccgagcggtc
      781 aaccttcgtc gggtccttgc cggagaaagc gcctccgccg tgcggggccg accccccgta
      841 tgtgtcgacg atgatcttgc gtcccgtaag tcccgtgtcg ccctgtgggc cgcccgtcac
      901 gaaccggccc gtcgggttaa ccaggatctc ggccacgctc tcgtcgaaga ggtcctcagg
      961 catgacggga cgaatcaccg tctccaccag gtcgtccctt atatgggctt cagccccgtc
     1021 gcggtgctgg gcggagatga ggaccttctc gatcccgatg ggcttgtctc cttcgtagcg
     1081 cactgtcacc tgactcttgc cgtcaggacg caggtagtcc aggtcgccgc tctttcggac
     1141 cccggagaga cggcgtgtca gggcgtgggc aagtgtgatc gggagcggca tcaactcctc
     1201 cgtctcctcg caggcgtagc cgaacatcat cccctggtcg cctgccccta tctcgtccag
     1261 gtcctggtcg ccatctctga cctcgagggc cctgtcgacg ccctgggcga tatccgccga
     1321 ctggggatct atcgagacga tcacaccgca cgtctgggca tcgaagccga acttggccct
     1381 ggtgtagccg attctcgcga ccttctcgcg cacgagggtg gggatgtcca cgtaagtcga
     1441 tgtggtgatc tctccggcca cgacgacgag gccggtggtc actaatgtct cgcaggcaac
     1501 cctgctcatc gggtcgtcag cgagcgcggc gtcgaggatg gtgtcggaga tctggtccgc
     1561 tatcttgtca ggatgaccct ctgtaaccga ctctgaagtg aacaggtgcg aggttttcag
     1621 tttctggtcg tccgccgcgt tccaggtagt agtgtacgcc gttcgctcca ccatctctaa
     1681 ctctgcctct ccttatccat ctccactatc atgctgtcga ggatcacacg agccacctct
     1741 tccttcgagg cgcggggtac gaagcgctcc ccctcaggac ccaccacgta gacctcgttc
     1801 tcgtcggcgc cgaagccgac gccttccctg gagatatcgt tgccgactac gagatccgct
     1861 cctttgcttg cgagcttctc gcgggcgtcg gtcacggggt ccccgtgcgt cgccgcgaat
     1921 cctaccacaa agaggtcggg ataccgctcc ctgacagcct tgagaatgtc ggaggtcggg
     1981 gcaagatcca ggctcgtcgt cccagtgccg cgccggatct tgtcctttac gggtgaggca
     2041 ggcgtgaagt ccgagacggc cgccgccatg acgagcgcat gcgcgttccc ggcccgctcc
     2101 aggaccgcct cctgcagatc ctctactgat tcaaccggta aggtctcggt ccctggttcc
     2161 acgctctcta tgttggcggc cacgacggtc acgtcagcgc ctagccgcag ggcctcgcgg
     2221 gccaccgaga gccccatctt ccctgaggaa cggttcccga taaaacgcac gctgtctatt
     2281 ggctccctgg tgccgccagc ggtaatgagc aaccgccgcc cgctcatcgg cccaccgagg
     2341 cttccgagta gtccggcgac gacctctcct gctggggcca tgccaccgtc atgcccttcg
     2401 atgatgcggc agccgtcttt ccgcaggagc ctcaggttct ctcgcacggc agggtgaccg
     2461 gccgtaccgg ggtc
//
LOCUS       DATAIR010000647         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_137490, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000647 DATAIR010000000
VERSION     DATAIR010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137490"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHM69_15340"
     CDS             complement(<1..228)
                     /locus_tag="VHM69_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX2741816.1"
                     /translation="MSRRVLVAGIGNIFLGDDGFGVEVVKRLAGRVLPEGVEVVDFGI
                     RGMDLAYALHEDYDLVVFVDATPRGGEPGTVY"
     gene            complement(221..532)
                     /locus_tag="VHM69_15345"
     CDS             complement(221..532)
                     /locus_tag="VHM69_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741817.1"
                     /translation="MDFFQEQEGRPELRSVVVEGVELTKGSRVMLWPRSGGDVMDIAL
                     AGKIAFVEAIEQDYEEKVHLSVTLEDDPGRDLGDAHILGHRFFFSPDEVVPLDEGAAS
                     E"
     gene            complement(534..1544)
                     /locus_tag="VHM69_15350"
     CDS             complement(534..1544)
                     /locus_tag="VHM69_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741818.1"
                     /translation="MDVVKKIAEAVLYEGYVLWPYRKSAMKNQQRWTFGGVYPRAYSE
                     ARGEDDPWLMQTQCLVSGDGESKIEVRVRFLHVTERKVGRKVGETLEYVDQLRLGEDV
                     YLPWDEATEREIAVGRFILSDLVEKPRRAGIDIPRGSREEVLTSPTDENTGVLVRSWR
                     ALGGTVEVEAEALREGIYMVTVRITNTTPWEGEDRESTLRQTFVSTHTVLEVEGGDFV
                     SMTDPPEELQLLVDVCENLKTWPVLVGEEGDRSMLLSSPIILYDYPRIAPESPGDMFD
                     GGEIDQLLILNILTLTDEEKAEMRASDPRAREILERCESLSQEELMNLHGAIREFRVL
                     RD"
BASE COUNT          287 a          565 c          453 g          349 t
ORIGIN      
        1 gtagacggtt cccggctcac ctccccgcgg agtggcgtcg acgaagacaa cgagatcgta
       61 atcctcgtgc aaagcgtacg cgaggtccat gccgcgaatg ccgaagtcca cgacctcgac
      121 gccctcgggt agaacacgtc cggcgagccg cttcacgacc tcgaccccga atccgtcgtc
      181 gcccaggaat atgttgccga tgcctgcgac gaggacgcgc ctactcactc gcggcccctt
      241 cgtcgagcgg cacgacctca tcaggcgaga agaagaaacg gtgcccgagg atgtgggcgt
      301 ctcccaggtc cctgcccggg tcgtcctcca gcgtgactga caggtggacc ttttcctcat
      361 agtcctgttc gatagcctcg acgaacgcga tttttccggc gagcgcgatg tccatgacgt
      421 ccccgccgga gcgcggccag agcatgacgc ggctcccttt cgtaagctcg acgccctcca
      481 cgaccacgct ccgaagctcg gggcgtccct cctgctcctg gaagaaatcc atcctagtcc
      541 ctcaacactc tgaactcgcg gatagccccg tgaaggttca tcaattcctc ctgtgagagg
      601 gactcgcacc gctccaggat ctcgcgcgcc cgcgggtcgg aggcgcgcat ctcggccttc
      661 tcctcgtccg tgagggtgag gatgttcagg atcaggagtt gatctatctc ccccccatcg
      721 aacatgtcgc ccgggctctc cggggcgatc cggggatagt cgtagaggat gatcggggag
      781 gagagcagca tgctcctgtc cccttcttct ccgacaagca caggccacgt cttcaggttc
      841 tcacacacgt ctacgagcaa ctgcaactct tccggcgggt ccgtcatcga gacgaagtct
      901 cctccctcca cctccagcac agtgtgggta gagacgaagg tctggcggag cgtgctctcc
      961 ctgtcctctc cttcccaggg agtggtgttg gtgatcctga cggtcaccat gtagatcccc
     1021 tcccgcaaag cctcggcttc gacctcgacc gttccgccca gcgcgcgcca gctacgcacg
     1081 agaacacccg tgttctcatc cgtcgggctg gtcagtactt cctctctgct tccacgtggg
     1141 atgtctatac ctgcccgtct gggcttctcc accagatcgg agagtatgaa ccgtccgacg
     1201 gctatctctc gctcggtcgc ttcgtcccat ggtagataga catcttcccc gagacgcaac
     1261 tgatcgacgt attccagggt ttcgccgact tttctgccga ccttgcgttc ggtgacgtgc
     1321 aggaaacgca ccctgacttc gatctttgac tctccgtctc ctgagaccag gcactgcgtc
     1381 tgcatcagcc aggggtcgtc ctcgcccctg gcctcgctgt aggccctggg gtagactcct
     1441 ccgaacgtcc agcgttgctg gttcttcata gcggactttc gataaggcca caacacatat
     1501 ccttcgtaga gcaccgcctc ggcgatcttc ttgaccacgt ccatttagcg ctccacttcc
     1561 tgctcgctgg cgcggagcaa tgcctccacg gtggcttccc aggtgcgtag tcccatacgt
     1621 agcttgtagt cgtagagccg gtcgaaggcg tctc
//
LOCUS       DATAIR010000648         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_251086, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000648 DATAIR010000000
VERSION     DATAIR010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251086"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHM69_15355"
     CDS             complement(<1..428)
                     /locus_tag="VHM69_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX2741819.1"
                     /translation="MLSRVKDGLSAGYVRLRTNVWPVSQAAVAASLAYFLADVVIGHG
                     QPFFAPIAAVICLSVTLGHRNRRAVELVLGVAVGLMVADLLVLVIGNGTLQIAVVVLL
                     AMAAAVFFGGGTILVNQAAVSAILVVVLQPPGAVFSPDRFV"
     gene            640..906
                     /locus_tag="VHM69_15360"
     CDS             640..906
                     /locus_tag="VHM69_15360"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2741820.1"
                     /translation="MSRERVVETALRHGVRSVRVFGSVARGTDAEGSDLDLLVEFEPG
                     RDLFDLIELKYELEEVSGRSVDVVTEKSLSPHIREKVLREAMPL"
     gene            903..1247
                     /locus_tag="VHM69_15365"
     CDS             903..1247
                     /locus_tag="VHM69_15365"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX2741821.1"
                     /translation="MTDDEVYLRHFLESLERIEAYTAGGREYFMHSSMAQDATVRNLE
                     VVGEATKRLSPEIRSAHPEIPWRNMARMRGVLIHDYMGVDLEIVWEVVENRLRPLRDH
                     LSPLFEPDDPER"
     gene            1318..1812
                     /locus_tag="VHM69_15370"
     CDS             1318..1812
                     /locus_tag="VHM69_15370"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX2741822.1"
                     /translation="MRRQRVEPGQGQESVWDYPRPPRLEDVDRKVKVVFGDVTLAYTT
                     RAKRVLETSHPPVYYVPPEDIRMEHLEPSAGGSYCEWKGMASYYDVVTEERSERRAAW
                     FYADPAEPFASLEGYVAFYPAKMDACWVGGEKVEAQEGGFYGGWITPDIVGPFKGAPG
                     TRGW"
     gene            1816..2451
                     /gene="pdxH"
                     /locus_tag="VHM69_15375"
     CDS             1816..2451
                     /gene="pdxH"
                     /locus_tag="VHM69_15375"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX2741823.1"
                     /translation="MDASRLRKEYTRAGLVEADVDPDPVLQFHNWFEKVIEADLHEPN
                     AMILATASREGRPSARIVLLKGYDERGFVFYTNYEGRKAREIEANPLCALLFYWGELE
                     RQVRIEGRACRISEEESDAYFLSRPRGSRLAAWASEQSRPVESRSILEERVRALEADY
                     EGLEVPRPPFWGGYRVEPEVIEFWQGRKNRLHDRLVYRRTEGEWRIERLQP"
BASE COUNT          520 a          738 c          836 g          414 t
ORIGIN      
        1 acgaagcggt caggcgagaa gacggccccg ggaggctgca acacgacaac gaggatcgcc
       61 gagacggccg cctggttgac caggatcgta cctcccccga agaacaccgc cgcggccatc
      121 gcaaggagca cgacgaccgc gatctggagc gtcccgttcc ctataaccag taccagaagg
      181 tcggccacca tgagcccaac ggccaccccg aggacgagct cgacggcgcg cctgttcctg
      241 tggccgagcg tgacgctgag gcagatgacg gcggcaatgg gggcgaagaa gggctgcccg
      301 tgcccgataa ctacgtccgc caggaagtaa gccaggctcg ccgccacagc cgcctgcgac
      361 accggccaca cattggtcct gaggcggacg taccccgcgg aaagaccatc cttgaccctg
      421 gaaagcaaac gtaccctctc tcacggtgcc gcccgcacga acccttcgtg aactatacac
      481 cgtcgcgtta gcgacctact ctgcctcacc ggcacgtgag agaaggacgc gtcctcacca
      541 ccggccatga cgctccgctt tgcttgccgc tgctatcatt cagtgcgagc tggctgagaa
      601 cgagtggtga atgggaaaat gtcgcaactc tttgggaata tgtctcggga acgggtcgta
      661 gagacggccc tccgacacgg tgtccggagc gttcgggtgt tcggctcggt ggcgcgaggc
      721 acggacgcgg aagggagcga cctcgacctt ctcgtcgagt tcgagcctgg acgagatctc
      781 ttcgacctga tcgaattgaa atacgaattg gaggaagtgt cgggccgaag cgttgacgtc
      841 gtgacagaga agtcgctctc gccgcacata cgtgagaagg tgctgcgcga ggcgatgccc
      901 ctgtgaccga cgatgaggtc tatctgcggc actttctgga gagcctggag cgcatcgaag
      961 cgtacaccgc cgggggcagg gaatacttca tgcactctag catggctcag gacgcgaccg
     1021 ttcgcaatct ggaggtagta ggcgaggcga caaaaagact ctcgccggag atccgctcgg
     1081 cacatccgga gataccgtgg aggaacatgg ctcggatgcg cggcgtactg attcacgatt
     1141 acatgggcgt ggacctggag atcgtttggg aagtagtcga gaatcgactc agaccgctgc
     1201 gggaccacct ctccccgctc ttcgaacccg atgacccgga gcggtgaacg cgccgagact
     1261 gctggtacag gagcaagccg ccttactagc tccctaaaac aacatcgagg aggtctaatg
     1321 cgtcgtcagc gagtagaacc cggtcaggga caggaatccg tctgggatta tccgcgcccg
     1381 ccgaggctgg aggacgtgga caggaaggtc aaggtcgtct tcggcgacgt gacgctagcc
     1441 tacactacaa gggcgaagcg cgtcctcgag acgagccacc cgcctgtgta ctacgtcccg
     1501 cccgaggaca taaggatgga gcatctcgag ccctcggcag gtggttcgta ctgcgagtgg
     1561 aagggcatgg ccagctacta cgacgtggtt acggaggagc ggtcggagag gcgggcggcc
     1621 tggttctatg ctgacccggc ggagccattc gcgagcctcg aaggctacgt ggccttctat
     1681 ccggcgaaga tggacgcctg ctgggtcggc ggggagaagg tcgaggcgca ggagggaggc
     1741 ttctacgggg gttggatcac cccggacata gtcggccctt tcaagggcgc tccaggaacc
     1801 cggggctggt agaccttgga cgcctcacgc ctccgcaagg agtacactcg tgccggcctt
     1861 gtcgaagccg acgtggaccc tgatcctgta ctccagttcc ataactggtt cgagaaggtg
     1921 atcgaagcgg acctgcacga gccgaacgcg atgatcctcg ccacagccag cagagaaggc
     1981 aggccctcag cccgcatcgt cctcctcaag ggctacgacg agcggggttt cgtcttctac
     2041 acaaactacg agggacgcaa ggcccgcgag atcgaggcga accctctatg cgcgctcctt
     2101 ttctactggg gcgagctcga gcggcaggtc aggatcgagg gccgcgcgtg ccgtatctcc
     2161 gaggaggagt cggatgccta cttcctgagc cgtccgcgag ggagtcgtct cgcagcgtgg
     2221 gcttcggagc agagccgccc ggtcgagagt cgaagtattc tcgaagagcg ggtgcgggcg
     2281 ctggaggcgg attacgaggg gcttgaggtc ccgcgcccac cgttctgggg aggatacagg
     2341 gtcgagcccg aggtgatcga attctggcag ggacgcaaga acaggctgca cgaccgtctc
     2401 gtctaccgtc gtaccgaggg agaatggagg atagagcgtc tgcaaccgta gggacgctgg
     2461 tggccccgcc ttgctgctaa gatgcgttct ggaacagatg gagaggag
//
LOCUS       DATAIR010000649         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_459854, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000649 DATAIR010000000
VERSION     DATAIR010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459854"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /gene="accC"
                     /locus_tag="VHM69_15380"
     CDS             <1..1428
                     /gene="accC"
                     /locus_tag="VHM69_15380"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2741824.1"
                     /translation="RRVRPDALLPAARRTVVIGKVLVANRGEIALRIIRSCRELGVRS
                     VAVYSTADTDSLHHELADESICIGPPPAVSSYLNVPAIISAAELTGADAIHPGYGFLA
                     ENARFAEICERVGLKFVGPSSRTIARMGDKAEARDAAAEAGVPVTPGSDGPCESVEEV
                     KRVGAEIGYPLVIKASAGGGGKGMRVVEDEAGVESAYLSASREAGASFGDGAVYVEKY
                     LETLRHVEVQVLADSYGETVTFPERDCSIQRRYQKLIEESPAPGLPEDVRDGLMAASA
                     DLIGSLDYEGAGTVEFVYADGEFYFIEMNTRLQVEHPVTEMISGVDIVAEQLKVASGE
                     RLEIPADALSPDGHAIEFRINAEDPLRNFLPQAGLVDVYNPPGGPGVRVDSHLYAGYR
                     VPPHYDSLLAKLIVHARDRDGAIRRGLRALDEFAVSGMETTLPLHLAVLEDEEFRRGG
                     VTTRFLADRELESEGGLLRLHRKSA"
BASE COUNT          309 a          546 c          552 g          266 t
ORIGIN      
        1 cgtcgagtac gaccagacgc tcttctacct gcggcccgac gaacagtagt gatcggcaag
       61 gtcctcgtag cgaaccgcgg cgagatcgcc ctgcgcataa tccggtcctg ccgcgagctc
      121 ggggtgcgca gcgtcgcagt ctactccacc gctgacaccg attccctgca ccacgagctg
      181 gccgacgagt ccatctgcat aggcccaccc cctgcggtga gcagctacct gaacgtgccc
      241 gcgatcatct ccgccgccga actcacgggc gcggacgcca tccatcccgg ctacggtttc
      301 cttgccgaga acgcccgctt cgccgagatc tgcgagcgag tcggcctgaa gttcgtcggc
      361 ccgagctccc gcactatagc caggatgggc gacaaagccg aggcacgcga tgccgcggct
      421 gaagcgggcg tccccgtgac ccccggctcc gacggcccct gcgagagcgt tgaggaggta
      481 aagcgagtcg gcgccgagat cggctatccg ctggtcatca aggcgagcgc aggcggcggg
      541 gggaagggca tgcgcgtggt ggaggacgag gcgggtgttg agagcgccta cctcagcgcg
      601 agcagggagg ccggggcaag cttcggcgac ggcgcggtct acgtcgagaa gtacctcgaa
      661 acgctcaggc acgtcgaggt tcaggtcctc gccgactctt acggcgaaac ggtaaccttc
      721 cccgagaggg actgctccat ccagcgccgc taccagaagc tcatcgagga atcgccggcg
      781 ccgggtctgc ccgaggacgt gagggatggt ctcatggcgg cctctgcaga cctgatcggc
      841 tccctcgatt acgagggggc ggggacggtg gagttcgtct acgccgacgg ggagttctac
      901 tttatcgaga tgaatacccg gctccaggtc gagcatccgg tgacggagat gatctcgggt
      961 gtggacatcg tggcagagca gcttaaggtc gcctcgggcg agcgtctgga gatccccgcg
     1021 gatgccctca gccccgacgg gcacgcgatc gaattcagga tcaacgccga ggatcccctc
     1081 cgcaatttcc tgccacaggc cggcctcgtc gatgtctaca acccgcccgg aggccccggc
     1141 gtcagagtcg actcgcacct ctacgccggt tacagggtgc caccgcacta cgattccctt
     1201 ctggcgaaac tcatcgtgca cgcgcgcgac cgcgatggtg cgatacgccg cggcctccgg
     1261 gcactggacg agttcgccgt ctcgggcatg gagacgaccc tcccgctgca cctcgccgta
     1321 ctggaggacg aggagttcag gcggggcggc gtcaccacgc ggttcctcgc ggatagggag
     1381 ctcgaaagcg agggcggcct cctgcggctc cacaggaaat ccgcctgaat aacgttggcc
     1441 gcctctacgc gccgacaggc atgttaatct ttacgacttg atcctcggct agaagaggga
     1501 gaactttgtc ggaagacgtc gcgaaggaga agaacggagg gcgcctgctc ttcggtggga
     1561 tggcccgctg ggacgggctc gacctcttcg gcccgaacta catgagcgtc gcgtaccgcc
     1621 ggaagggtga gatccacgtc aaggtcgaac cctccaaaat gcgcaagccc acg
//
LOCUS       DATAIR010000650         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_342812, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000650 DATAIR010000000
VERSION     DATAIR010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342812"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VHM69_15385"
     CDS             complement(<1..741)
                     /locus_tag="VHM69_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2741825.1"
                     /translation="MLRCAVVHDLLIPETSKEAKTDTRTGSQVEVKLVIPPGRTLEVF
                     GDRDSVLRRVEELVECEVVVRGNEIVLRGTEVAVERAEAVFDGLVGLAEEGHHPTPET
                     AERLFKMADSGGGREVLGDVILTHRGRRVAPKTRNQKAYTDAIRQHQVVFGIGPAGTG
                     KTYLAVALAVGALSRGEVSRIILTRPAVEAGESLGYLPGDVMAKVDPYFRPLYDALYE
                     MVDPAKFQAHLERGIIEIAPLAFMRGRTL"
     gene            complement(826..1269)
                     /locus_tag="VHM69_15390"
     CDS             complement(826..1269)
                     /locus_tag="VHM69_15390"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2741826.1"
                     /translation="MTAAERIQSDTKEAMKQRDRTRVGALRMLGAALKNAEIEAGRPL
                     TEQEEQTILRRQLKQRDESAEAFRKAGREERAAAESAEADIVRSYLPEPLSPEELEHI
                     VDRAVNETGATSMRDMGAVMSRAMELSEGRAEGREVSELVRGRLQ"
     gene            complement(1266..1610)
                     /locus_tag="VHM69_15395"
     CDS             complement(1266..1610)
                     /locus_tag="VHM69_15395"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX2741827.1"
                     /translation="MSETECLFCKIARGEMETEFVHVEDDVLAFNDINSKAPVHVLVI
                     PRQHVANLEEVGALPDAVVKRLFEVASAVAEKLGVTESGYAVRVNNGSDAGQEIFHLH
                     LHVMGGKRLGMP"
     gene            complement(1624..2304)
                     /locus_tag="VHM69_15400"
     CDS             complement(1624..2304)
                     /locus_tag="VHM69_15400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX2741828.1"
                     /translation="MDSALEPGGVLELPAGEAHHVLKVLRGRSGDPIEVADGAGRVFA
                     ARLRGGKVVDVQEDLATQEPVEITLYQAVPKGGRMDLVVEKATEAGATRIVPLLTERG
                     VVNPREGKIGRWRRVVAAAARQSLRLSVPEVTEPEPFEDAVLNVGETGVLLHNASELR
                     PVEAVVDTPASLFIGPEGGWSEGELRFAEEAGLAFGGLGPYRLRSETAGIVAVARASA
                     ALQSRVKP"
     gene            complement(2343..3476)
                     /gene="dnaJ"
                     /locus_tag="VHM69_15405"
     CDS             complement(2343..3476)
                     /gene="dnaJ"
                     /locus_tag="VHM69_15405"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2741829.1"
                     /translation="MATKRDYYEVLGVSREASEAEIKKAYRRAARSHHPDANPGDHEA
                     EDRFKELTEAYEVLANPEARRAYDTYGHQVPRGGAGGYPGGGDPFGGFEDIFEAFFGN
                     RFGGNSFFGASTAQRPSRGSDAEVEVEVSLREAAFGIDREVDVQVVKNCGVCDGVGGT
                     ESHVCPTCGGAGAVRTVRESILGQMVSTQTCSSCGGRGRVIDVYCDNCRGSGRVSEVQ
                     TRRLRVPEGIESGMRLRVSGGGHAGEPGAPAGDLYVKVKVKEDPELLRDGEDLIHRMK
                     VSFVGAAIGTEVEVPTLDGTKPVRIEPGTQPGATLTLRGEGMPRLRRRGRGDLKVVVD
                     VMVPTRLTGEQRELLERFEMVSGEETYNGSGGSFFDRLRGVFR"
BASE COUNT          591 a         1289 c          988 g          707 t
ORIGIN      
        1 caacgtgcga cctctcatga atgccagggg tgctatctcg attataccgc gctcgaggtg
       61 cgcctggaat ttggcagggt cgaccatctc atagagcgca tcatacagcg gcctgaagta
      121 tgggtcgact ttggccatca catcacccgg cagataaccg agcgactcac cggcctcgac
      181 ggctggcctg gtcaatatga tcctcgagac ctcccccctg ctgagagccc cgacggcaag
      241 ggcaactgcc aggtatgtct tgcccgtccc agccggacct attccgaaaa ctacctgatg
      301 ctggcgaatg gcatcggtat aggctttctg gttgcgggtc ttcggcgcga cgcggcgtcc
      361 ccggtgcgtc aggatcacat ccccgagcac ctctcttccc ccgccgctat ccgccatctt
      421 gaagaggcgc tccgcggtct caggggtcgg atggtgcccc tcctcggcaa gcccgacgag
      481 gccatcgaag acggcctcag ccctctccac ggccacctcc gtgccgcgaa gcacgatctc
      541 gttccccctc acaacgacct cgcactcgac tagctcctcg acccgtctga gaactgagtc
      601 ccgatccccg aagacttcga gagtccttcc cggcggtatg acgagcttga cttctacctg
      661 gctgccagtt ctagtgtccg tctttgcctc ctttgaggtt tcaggtatca ggagatcatg
      721 aaccactgca cagcggagca atatatgcgg acagcgtcgg attccgcggt ggcggaggca
      781 ctatccgacg agaagctgac ggctgaagcc tgatacctgt agccttcatt gcagcctacc
      841 tcgcaccagt tcggagacct cgcgcccttc cgcgcggcct tctgagagtt ccatggccct
      901 gctcatgacg gcccccatgt ccctcatgct cgtcgccccc gtctcgttca ctgccctgtc
      961 cactatgtgt tcgagctcct ctggcgagag gggttccggc aggtaggagc gcacgatatc
     1021 cgcctcagcc gactcggccg ccgcccgctc ctcgcgccct gctttgcgga acgcctccgc
     1081 cgactcgtcg cgctgcttga gctggcgcct gaggatcgtc tgttcttcct gctcggtgag
     1141 cggcctcccc gcctctatct cggcgttctt gagcgcggct ccgagcatcc gcagcgcgcc
     1201 gacgcgggtc ctgtcgcgct gcttcatggc ctctttggtg tcgctctgta tcctctccgc
     1261 cgcggtcacg gcatccccaa cctcttgcca cccatgacgt gcaggtggag gtggaagatc
     1321 tcctgccccg catcggaccc gttgttgacc ctgacggcgt agccactctc cgtgaccccg
     1381 agcttctcgg cgacggcgct cgccacctcg aagagtcgct tgacaacggc atcaggcaag
     1441 gctccgacct cctcgaggtt ggcgacgtgt tgcctcggga tgacgagaac gtggacgggg
     1501 gccttgctat ttatgtcgtt gaaggcaagc acatcgtcct cgacgtggac aaactcggtc
     1561 tccatttccc ctcgggctat cttacagaat aagcactccg tctcgctcac gactgacctc
     1621 ctcctacggt ttgaccctgc tttgcaacgc ggccgacgcc cgcgcgacgg cgacgatgcc
     1681 agcggtctcg ctacggagcc tgtatggtcc caacccgcca aacgcaaggc cggcctcctc
     1741 cgcgaaccgc aactcaccct cgctccaacc gccttccggc ccgataaata ggctcgcggg
     1801 agtatcgacg acggcctcga cgggccggag ttcgctggcg ttgtgcagaa gcacccctgt
     1861 ctcccccacg ttcaggaccg cgtcctcaaa cggctcaggc tccgtcacct ccggcacact
     1921 tagcctgagc gactgtcgcg ccgcagccgc caccactcga cgccagcgcc cgatcttccc
     1981 ctcgcgcggg ttcaccacac cacgctcggt aaggagcggc acgattctag tggccccggc
     2041 ctcggtggct ttctcgacca cgagatccat gcgccccccc ttcggcaccg cctgatagag
     2101 cgtgatctcg acaggttcct gggtcgcgag gtcttcttgc acgtccacca cttttccgcc
     2161 acgtagccgg gctgcgaaga cccgtccggc gccgtccgcg acttcgatgg gatccccact
     2221 ccggcctcgc aggactttga gaacgtgatg ggcctcgccc gcaggtagct ccagaacccc
     2281 accgggttcc agtgcactgt ccacgaagat acgggttatg ggcgtaggcg taacagagcc
     2341 ccctacctga acactcccct gagacggtcg aagaaggagc ctccacttcc gttgtaggtc
     2401 tcttcaccac tcaccatctc gaacctctcg agcaactccc gctgctcccc cgtcagacgc
     2461 gtcggaacca tgacgtcgac gacgaccttg aggtccccac gtccacgccg cctcaaacgc
     2521 ggcatacctt cacccctgag cgtgagcgtc gcccccggct gggtgcccgg ctcgatcctg
     2581 acgggcttcg tcccatcaag ggtgggcact tcgacctcag tccctatcgc cgcccccacg
     2641 aaactcacct tcatcctgtg gatcaaatcc tcaccgtcgc gcaatagctc gggatcttct
     2701 ttaaccttca cctttacgta taaatccccc gccggagcgc cgggttctcc tgcgtgcccc
     2761 ccacctgaca cacgcagcct catgccgctc tcgatccctt ctggcacacg caggcggcgc
     2821 gtctgtactt cggagactcg tccgcttccg cgacagttgt cgcagtacac gtcgatgaca
     2881 cgtccccgcc ctccgcaaga cgagcacgtc tgggtgctca ccatctggcc caggatactc
     2941 tcgcgcaccg tccttacggc accggcgccg ccgcacgtgg gacagacgtg tgattcggta
     3001 ccccctacgc cgtcgcagac tccgcagttc ttgacaacct gaacgtcgac ttcacggtct
     3061 attccgaacg ccgcctctcg caaagaaacc tctacctcga cctcagcgtc actgccgcga
     3121 ctcgggcgtt gcgccgtcga ggcaccgaaa aaggagttgc cgccgaatcg gttgccgaag
     3181 aacgcttcga atatatcttc gaaaccgccg aacgggtcgc ccccgccggg gtagccgccg
     3241 gcgccgcctc gcggcacctg gtggccgtag gtatcgtaag cgcgtctggc ttcagggttc
     3301 gcaaggacct cgtaggcttc ggtgagttcc ttaaagcggt cttcggcctc gtggtcgccc
     3361 gggttggcgt ccgggtggtg agagcgggcg gcgcggcggt aggctttctt gatctccgcc
     3421 tccgacgcct cccgggacac gcccagaacc tcgtagtagt cgcgcttggt tgccacctag
     3481 aacctggcgt ccaggcagtt tgtcagggct tcggcggcgg agcggaccgt cgttatagcg
     3541 acgtcgtagt ccatgcgctt ggggccgatc aggct
//
LOCUS       DATAIR010000651         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_414921, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000651 DATAIR010000000
VERSION     DATAIR010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414921"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            544..2547
                     /locus_tag="VHM69_15410"
     CDS             544..2547
                     /locus_tag="VHM69_15410"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2741830.1"
                     /translation="MTPPVNEDQRTTPARDGREAGRLAALEYLRAVRPGVDHVLQELV
                     DEVRGVFGTELCMVNLNLSDVQYFRAWSGDLPPDLAEARQDPIEHSMCQYVVKEEMPL
                     VVEDFLATEEFREQYWCVNYGVRFYAGTPLVTSAGHAIGTLCLLSTRSFDFDEDQLRV
                     LGAFARAVVGRLELLGALEREQTFRKEQTQRSRQLQQTLDSLSAHIAILDESGEIVAV
                     NDAWRTFARANDSDPERVSEGVNYLQVCGATSGSAEAVAFERGIRAVLSGRREDFELE
                     YPCHSPTERRWFIGRVTRFSGGERPWAVVAHENITQRKMAEQALRDQKVLLETILGQA
                     ADAIVVSDDGGTFTFANAAARNMAMRDPEGTTFEETSEVWGVAHSPDGRRIPLGESSI
                     ARALRGETTLGRETRMVRPDESHYDVLISAAPLRDSEGEIVGAVAGLLDITERKLAEQ
                     ERDRLRAREIEARTQREERRRIARDLHDVVLQDLSGTLQSLRLTHLRKDMHPQVDLEG
                     ELEALGRATSGLRNAIYDLRHEKKRPFAKSVESLVEFSSHAASGCEIGLSVQGRLPAE
                     LPDEVGVELLRVLQEALINARRHSGARNVEVRLRAEGGEILAEVIDDGRGFDPASVRA
                     GVGLSAMRERVEVLGGEISIQSRPGEGTMVRVNVPAGGGTPGP"
     gene            complement(2510..3214)
                     /locus_tag="VHM69_15415"
     CDS             complement(2510..3214)
                     /locus_tag="VHM69_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741831.1"
                     /translation="MRFLSDGEGAIRVAIADDQRLFTKGLSGLVDMLTGVEVVGVAYD
                     GEEAVALCRREEPDVVLMDISMPKMDGIDATREIKDLLPQTAVIILTGHEEDEHVFEG
                     IKAGAQGYLLKDSEPEDLARAIHTVNAGDTIIAPDLAQKMLRTFEGGRPGSPRLAPPP
                     LTERELEVIKALAQGMSDRQIAGSLGISEKTVRNHTSNIYRKLHIFDRTQAVIYAVRE
                     GVIDIKDLEYRPPQGH"
     gene            complement(3546..>3861)
                     /locus_tag="VHM69_15420"
     CDS             complement(3546..>3861)
                     /locus_tag="VHM69_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2741832.1"
                     /translation="EDAQSYIGTPYGAYGFDCSGFTSAVFADFGVYLPDSPYDQYAYG
                     TPSYGEAGDLVFFDEHGYGISHVGIATGYGTMVHSSTYYGEVVESPISSVPGYVGAVD
                     VY"
BASE COUNT          732 a         1149 c         1270 g          710 t
ORIGIN      
        1 agaactcttc ggaatctatg gggaacacta cctgtgcctc gcggcctgga tagcgtccct
       61 tcacgatgct cacggcccgg tagatgctat ctgcctgcac cgccaccttg atgggagaga
      121 tcccttcgag cacttccaca cagaccttta tcatctgccc gcgcctctat gaacagagac
      181 atatctctga acgggtcccc gatcgcccga cgtgtaccaa cgggcgctcc ggggaggagg
      241 gctcgggaag gcggcatttg aagggctatg tagggaaaga aggcgccgcc gggtcccgag
      301 ctcgtcgatg gcgcaccgca ggcagcaggt aaaggtgtgc tgctccgtgt atgtgccggc
      361 gaccatctca cagacgagta tggtccgcag ccgccgggtt tccatcggac ttttcgacta
      421 ttcactgccg gtaatggtgg ctagggcgat tgccctatca aatctcgagg gctctcatga
      481 ctcgtgtttg gggagcagca tggtattctg agcgtcggta aacgcatgaa cgagggtgct
      541 ttcgtgaccc cgcctgtcaa tgaggaccag cgcaccaccc cggccaggga cggacgcgaa
      601 gctggacgcc ttgcggcgct tgaatacctg agggcggtca ggccgggtgt tgaccacgtc
      661 ctccaggagc tggtggacga ggtgcgtggc gtgttcggta cggaactttg catggtcaac
      721 ctcaatctct cggacgtgca gtacttcagg gcgtggtcgg gggacctgcc tccggatctg
      781 gccgaagcca ggcaagaccc catcgagcac agcatgtgcc agtacgtggt gaaagaagag
      841 atgcctctcg tcgtggagga cttccttgcc acggaagagt tcagggagca atactggtgc
      901 gtgaactacg gcgtccgttt ctacgccggt acgcccctcg ttacctcggc cggacacgcg
      961 atcggcactc tttgcctgct cagtaccagg tctttcgact tcgacgaaga tcaactcagg
     1021 gtgctcgggg cgttcgccag ggccgtcgtc gggcggctgg agttgctcgg ggcgctggag
     1081 cgagagcaga ccttcaggaa agaacagacg cagcgcagcc ggcagttgca acagacgctc
     1141 gacagcctct cggcgcacat agccatcctc gacgagtccg gagagatcgt cgccgtgaac
     1201 gacgcttgga ggacgttcgc ccgggcgaac gacagcgacc ctgagagagt ctcagagggc
     1261 gtcaactacc tccaggtctg cggagccacc tcgggctccg cagaagccgt cgccttcgag
     1321 cgagggatca gggccgtcct ctcgggccgg cgcgaggact tcgagctgga atatccgtgc
     1381 cactcgccga ccgagcggcg ctggtttata gggagggtga cgaggttttc aggcggggag
     1441 aggccgtggg ccgtggtcgc ccacgagaac ataacccaac ggaagatggc cgaacaggcg
     1501 ctacgagatc agaaggtcct cctagagacg atcctgggtc aggctgcgga cgctatcgtc
     1561 gtcagcgatg acgggggcac cttcacgttc gccaacgcgg cggcgagaaa catggcgatg
     1621 cgtgaccccg aagggacgac gttcgaggag acctccgaag tgtggggggt ggcccactct
     1681 cccgatggtc gccgcatacc actcggggag tcgtccatag ccagggcctt gcgcggggag
     1741 accaccttag gcagggagac gcgcatggta aggcccgacg aaagccacta cgacgtcctc
     1801 atcagcgccg ccccgttaag agattcagaa ggagagatcg tcggggcggt cgcgggcctg
     1861 ctcgatataa ccgagcgcaa gctggccgaa caagagagag accgattgcg cgccagggag
     1921 atcgaggccc gcacccagag agaggagcgc cgcaggatag ccagggatct gcacgacgtt
     1981 gtcctccagg acctctcggg cacgctgcaa tctctgcgcc tgacccacct gcggaaggat
     2041 atgcaccctc aggtggacct cgaaggagaa ctggaggctc tcgggcgggc gacctcgggc
     2101 ttgcgcaacg ctatctacga cttgaggcac gaaaagaagc gacccttcgc gaagtccgtc
     2161 gagtcgctgg tcgagttcag cagccacgcc gcgtcagggt gcgagatagg tttgagcgtg
     2221 caaggacggc tcccggcaga actacccgac gaggttggtg tcgagttgct gcgcgtgctt
     2281 caggaagcgc tcatcaacgc caggcgtcac tccggggcga gaaacgtcga ggtcaggctg
     2341 cgggctgaag gcggggagat cctggccgaa gtcatcgacg acggcagggg gttcgacccg
     2401 gcctcggtcc gggccggcgt gggactatcg gcgatgcgcg agcgggtcga ggtcctggga
     2461 ggtgagatca gcatacagag caggcccggc gagggcacga tggtccgggt caatgtccct
     2521 gcggggggcg gtactccagg tccttgatgt cgatgacacc ttccctgacg gcgtagatca
     2581 ccgcctgcgt ccggtcgaag atgtggagct tcctgtagat gttggaggtg tggttgcgca
     2641 ccgtcttctc cgagatacct aaagagccgg ctatctgcct gtcgctcatg ccctgggcca
     2701 gggcctttat gacctcgagc tccctctccg tcagcggcgg aggggcgagt cggggacttc
     2761 ccggtctgcc gccctcgaag gtcctcagca tcttctgggc gaggtctggg gcgattatgg
     2821 tgtccccggc gttgacggtg tggatcgcgc gcgcgaggtc ctcgggttct gagtccttca
     2881 acaggtagcc ctgcgcgcct gcctttatgc cctcgaagac gtgctcgtcc tcctcgtggc
     2941 cggtcaggat tatcacggcg gtctgcggca ggaggtcttt gatctcccgc gtggcgtcga
     3001 tgccgtccat cttcggcatg gagatgtcca tcaggaccac gtcgggctct tctctgcggc
     3061 agagggcgac ggcctcctcg ccatcgtagg ccacccccac cacctccacg ccagtcagca
     3121 tgtccacgag gccggagagg cccttggtaa agagcctttg atcgtcagcg atcgcaacgc
     3181 ggatcgctcc ttcaccgtcg ctcaagaacc tcacacccct tcacctcttg gaagcccagc
     3241 acggtgttaa ctctatctgc tgcccgccgg atgtgtcacg ggattgaccg gtggagatcc
     3301 cgagtacttc atctctcgta tcatcttccg tgcgggcggc gttatcgtcg gatatttgca
     3361 tcacacgccg ctgggtaatt ccagctaggt cgtacgtccc gctagatagt cgcacaagac
     3421 gcggcaagtg aacgatcgac tgttcaatca ttctgtgcgc ctatctaggc tacggcgccg
     3481 tggcagtcaa gtggtgaaga tcagaggcca ggctccacag gaagcccggc ctctctcgtg
     3541 ctgtcctagt agacgtccac agcgcccacg tagccgggga cggaggatat gggcgactcg
     3601 acgacctcgc cgtagtaggt agaagagtgg accatagtac cgtagcccgt agctataccg
     3661 acgtgggata tgccgtagcc gtgctcgtca aagaacacca ggtcgccggc ctctccgtag
     3721 gatggggtcc cgtaggcgta ctggtcgtag ggggagtctg gcaggtacac gccgaagtcg
     3781 gcgaagaccg ccgaggtgaa gcctgagcag tcaaagccat aggcgccgta aggtgtcccg
     3841 atgtaggact gggcgtcttc c
//
LOCUS       DATAIR010000652         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_414932, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000652 DATAIR010000000
VERSION     DATAIR010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414932"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHM69_15425"
     CDS             complement(<1..884)
                     /locus_tag="VHM69_15425"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2741833.1"
                     /translation="MDNTYVSLEWRPELERPVLIAGFTGWNDAADAASVAVGALARSW
                     ETRRFGSFDGEEFFDFQTTRPQVKLVDGVTREIEWPENVLSATEPALEAAGGRGGILL
                     TGPEPNFRWRTFSAAVVDLAKELDVELVVTMGALLADVPHSRPVAVAANSQDATLVES
                     LGLSASRYEGPTGITGVLHRLCADGGLPSVSFWASVPHYLPAVPSAPAALALLEKLAT
                     LLGMQVETADLESTAKTYQEQVSVAVSQDSDLSSYVRMLEERFDSQADQGPRNLPSGD
                     ELAQELEGFLREQRNDEE"
     gene            complement(929..1501)
                     /gene="aat"
                     /locus_tag="VHM69_15430"
     CDS             complement(929..1501)
                     /gene="aat"
                     /locus_tag="VHM69_15430"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX2741834.1"
                     /translation="MRLTTDLLEVCYRAGAFPMADSYGRIEFYRSDPRSVLDLESLHV
                     SRSLGRVIRKGTYELRVDEDFEGVVRACADREETWINAEIVDAYVRLHFVGKAHSVEA
                     YSGGELAGGLYGVTLGGAFMGESMFSRMRDASKVCFVYLVERLKERGYELLDCQIQNP
                     HLASLGAVEIPEREYLRRLDHALSLDRTFV"
     gene            complement(1648..2634)
                     /locus_tag="VHM69_15435"
     CDS             complement(1648..2634)
                     /locus_tag="VHM69_15435"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX2741835.1"
                     /translation="MANAEAGLRGDAPLAAVRRGALVESVHRGRLAVCDPNGNVLEAV
                     GDHEGYLYARSSAKPLQALPLVLSGAADAFGLTDEELAVACASHNAEEPHLAAVRSIL
                     EKAGLTEGDLQSGAHPPLYAPDAAKLIRNGEEPRPIHGNCSGKHAGMLAVCVHEGYES
                     ATYREPDHPLQRRILGLIAEVCGLREDEVLVAGDNCGVPAFALPLRGFATGLARIATG
                     EGLSEELAGAALRIGDAMRGHPFMVAGTGRLDTELMDSTDLLVKGGADGVLAVGSREG
                     WGMALKISDGAQRAVRPAALAALGGRGIEVPDAVCTVRGLHGETVGEIRPLI"
     gene            complement(2627..2941)
                     /locus_tag="VHM69_15440"
     CDS             complement(2627..2941)
                     /locus_tag="VHM69_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741836.1"
                     /translation="MKAWERTDVPFRTAHLLRIDSYMDMAILAMWTMNPRVDVMIGMV
                     EASLRGESPGGKDDELLQQVRDLVREGRAYLAGGEFSVAMGRMRVAHDIVALHIIRLS
                     DG"
     gene            complement(2938..3759)
                     /locus_tag="VHM69_15445"
     CDS             complement(2938..3759)
                     /locus_tag="VHM69_15445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2741837.1"
                     /translation="METEHNIEALTGGAMAGVWSWVLGKIPAGVEVFDAHAHIGADVD
                     GRTMTADGMRDRMEAAGVKKSIVFPLNDPNARDDYSGPNDVVWHAYEEYPAQFVPFFR
                     LNPHLDYDTEFGRCVERGFMGLKLHPVSQKFELDDPRVVRLFSMAAEIDLPVLIHAGF
                     AMQHIVRPLLPTVEAHPGLRLILGHAAMVEVLAAARAFEGHPNVFFETSVVQAKDLYE
                     LFCSVDPSRICYGSDIPYGDLPSTLHSALAAADAAGVPDEALPGVLSENIRRWFP"
     gene            3950..4417
                     /locus_tag="VHM69_15450"
     CDS             3950..4417
                     /locus_tag="VHM69_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2741838.1"
                     /translation="MSIFPTKILLAADGSREAEIAARTAADLANSTNSELHVVTVAPG
                     NPDPVYHIHEGGFRYETYEEALEAVKGAAQKTLDEQVQKIEEAGGTVKEAHLKTGERR
                     DQAIVHLAEELGAGLIVLGSRGLGGVRRALMGSVSSSVVHHAHCPVLVVRPEK"
BASE COUNT          863 a         1545 c         1466 g          797 t
ORIGIN      
        1 tattcctcat cattgcgttg ctcgcggagg aagccttcga gctcctgggc gagttcgtcg
       61 cccgatggca gattgcgtgg gccttgatcc gcctgcgagt cgaagcgttc ttctagcatc
      121 ctgacgtagg acgaaaggtc agagtcctgg gagaccgcca cggagacctg ctcctggtag
      181 gtcttggccg tgctttcgag gtcggccgtc tctacctgca ttcccagtag tgtcgcaagc
      241 ttctcgagga gtgcgagcgc ggcaggggcg gacggaaccg caggcaggta gtgcgggacc
      301 gaggcccaga agctcacgga gggcagtcca ccgtcggcgc agagtcggtg cagcacgccc
      361 gttatgcctg tcggtccctc gtagcgcgag gcggagagac ccagggattc gacaagggtc
      421 gcgtcctggg aattggctgc taccgctacc ggacgcgaat gtggcacgtc ggcgagcagg
      481 gcgcccatcg tgaccaccag ctccacatcc aactctttag ccagatcgac gaccgccgca
      541 gagaaagtgc gccaccggaa gttcggctca ggtcccgtca gcagtatccc cccgcgtccg
      601 cccgcggcct ccagggccgg ctccgtcgct gagagcacgt tctcgggcca ttcgatctcg
      661 cgcgtcacgc cgtctaccag cttgacctgg ggccgcgtag tctggaagtc gaagaactct
      721 tcaccgtcga agctcccgaa acgccgcgtc tcccacgagc gcgccagggc tccgacagcg
      781 acgctggccg cgtcggcagc gtcgttccac cctgtgaaac ctgcgatcaa aaccggcctc
      841 tcaagctctg gccgccactc cagacttacg tacgtgttat ccatgaggct agcttaccac
      901 gcccgcccgg cactagattt actcgccctc agacgaaagt tctatccagg ctcagggcat
      961 ggtccaggcg cctcaagtac tcgcgctcgg ggatctcgac ggcccccagg ctggcaaggt
     1021 gggggttctg gatctggcag tctagcaact cgtacccacg ctcctttaga cgctccacca
     1081 gatagacgaa gcagactttg gaagcgtccc tcatgcgcga aaacatcgac tctcccatga
     1141 aagccccgcc aagagtgacg ccgtagagac cgcctgcgag ctcgccgcca ctgtacgctt
     1201 cgacgctgtg cgccttgccc acgaagtgca gcctgacgta agcatcgacg atctcggcgt
     1261 tgatccaggt ctcctccctg tccgcgcaag cccgcaccac gccttcgaag tcctcgtcca
     1321 cgcgcaattc gtaggttccc ttgcggatca cacgcccgag cgacctggag acgtgcagac
     1381 tctcgagatc gagcacgctg cgcgggtccg agcggtagaa ctcgatgcgc ccataggaat
     1441 cggccatcgg gaaagcgccg gccctgtagc acacctccag gagatcagtg gtcagcctca
     1501 tggacgtatg ttagcggcga gtcgcactcg aggctctacc cacggactca ctaccgagac
     1561 tcgaacagca atgctgacag cggagcccgc agggcgcggc gtgctgacca gctcgaaatg
     1621 cggaagtcaa gacatctgca tttcgcttca gatgagaggc cggatctcac cgacggtttc
     1681 gccgtgcagt cctctcacgg tgcataccgc atcgggcacc tcgatgcccc tgccgccgag
     1741 cgcggcgagc gccgcgggac gcacagccct ctgggcgccg tcggagatct tcagcgccat
     1801 cccccagcct tcccggcttc caacggcgag cacaccgtca gccccgcctt tgacgaggag
     1861 gtcggtagag tccatcagct cggtgtcgag ccttcccgtc cccgctacca tgaacggatg
     1921 cccgcgcatt gcatctccga tgcggagagc cgccccggcg agctcttcgg agaggccctc
     1981 gcccgtggcg atacgcgcga gtcctgtcgc gaacccccgc agcggcaggg cgaaggcggg
     2041 gacgccgcag ttgtcaccag ccacgagcac ctcgtcctcc cgcaggccgc agacctcagc
     2101 tatgagcccg agtatgcggc gctgaagcgg atggtcgggc tcgcggtacg tggcactctc
     2161 gtagccttcg tggacgcaga cggcgagcat gccagcgtgt ttgcccgagc agttaccgtg
     2221 tatcgggcgc ggctcctcgc cgttacggat cagcttcgcc gcgtcgggcg cgtacaacgg
     2281 cggatgggcg ccgctctgga ggtcgccctc ggtcaggccc gctttctcga ggatcgaccg
     2341 tacggcggcg aggtgaggct cctcggcgtt gtgcgaggcg catgccacgg cgagctcttc
     2401 gtccgtgagc ccgaaggcgt cggcggcgcc tgagaggacg agcgggagcg cctgtaaggg
     2461 cttggccgat gagcgggcgt agagatagcc ttcatggtcg cccacggctt cgaggacatt
     2521 gccattcggg tcgcagacgg cgagacgacc gcggtggacg ctctcgacga gtgcgccgcg
     2581 cctgacggcc gcgagcgggg cgtcgcctcg gagaccggcc tctgcgttag ccatcagaga
     2641 ggcgaataat gtgcagggca acgatgtcgt gggcgacgcg catccggccc atcgccacgg
     2701 agaactcccc gccggccagg tacgcccgtc cctccctgac gaggtcgcgc acctgctgga
     2761 gcagttcgtc gtccttgcca ccggggctct ctccccgcag gctcgcttcg accatgccga
     2821 tcatcacgtc gaccctgggg ttcatcgtcc acatcgcgag gatcgccatg tccatgtagc
     2881 tgtcgatcct gagcaggtgc gccgtcctga acggcacgtc ggtcctctcc caggccttca
     2941 agggaaccac cgcctgatgt tctcagagag caccccgggg agggcctcgt caggcacccc
     3001 tgcggcatcc gctgcggcaa gggcgctgtg cagggtcgac ggcaggtcgc cgtacggtat
     3061 gtccgagccg tagcagatgc gcgaaggatc gacggaacag aacaactcgt aaaggtcctt
     3121 tgcctgcact acagaggtct cgaagaaaac gttcgggtgc ccctcgaagg cgcgggcggc
     3181 ggcgagcacc tcgaccatcg cggcgtggcc gaggatcagg cgcaggccgg ggtgcgcctc
     3241 gaccgtaggc agtagcggcc tgacgatgtg ctgcatcgcg aagcccgcat ggatgagaac
     3301 gggcaggtcg atctcagccg ccatcgagaa caggcgcacc accctcgggt cgtccagctc
     3361 gaacttctgc gagacaggat ggagcttgag gcccatgaag ccgcgctcga cgcaacgacc
     3421 gaactccgtg tcgtagtcga ggtgcgggtt gaggcggaag aagggcacga attgggccgg
     3481 gtattcctcg taggcgtgcc agacaacgtc gttcggcccc gagtagtcgt cgcgggcgtt
     3541 cgggtcgttc agagggaaga cgatggactt cttcacgccc gccgcctcca tgcggtcgcg
     3601 catcccgtcc gcggtcattg tacggccgtc cacgtcggcc ccgatgtggg cgtgggcgtc
     3661 gaagacttcg acgccggcgg ggatcttccc cagcacccag gaccagacgc cggccattgc
     3721 cccgcccgtc agcgcctcga tgttgtgctc agtctccaaa gtgctcgccc atcctagagc
     3781 tcgtcatcca acgaccgtga aatggtagca ggggggatcg gatagtgctt attgggcctt
     3841 tccgagagca ctttcgaggt gagatctcac ctcgtccccg aggatgcaac ttgtcgcaac
     3901 cgatggcatt attagggtat atgaccccgc cgagcaagga gacgaccgca tgagcatctt
     3961 cccgaccaag atcctgcttg ctgccgatgg ttccagggag gcagagatag ccgcccgcac
     4021 cgccgccgac cttgccaaca gcacgaactc agagttgcac gtcgtcaccg tcgcgccggg
     4081 aaaccccgac cccgtctacc acatacacga aggcggcttt cgctacgaaa cctacgagga
     4141 agcactcgaa gcggtaaagg gagcagcgca gaagacgctc gacgagcagg tacagaagat
     4201 cgaagaggcg ggaggaaccg tcaaagaagc ccacctcaag acgggagaac gacgagatca
     4261 ggcaatcgtc cacctggctg aggagctggg cgccgggctc atagtcctcg ggagccgggg
     4321 cctcggcggg gtaagacggg cgctgatggg cagcgtctcc agtagcgtcg tgcaccacgc
     4381 ccactgcccc gtcctcgtcg tacgtccgga gaagtaaggg cgcgctagtc gttccaagaa
     4441 cctgcgtgaa cttcatcgat actttctctc gtgacgacgc gagaaggtct cgccctgaac
     4501 cgacgactct ttacgcgcaa gcgtcatttc atctgacagc ggcgttgatg tacgtggatc
     4561 caccgtctct ggccatcact tcggcgaacg agatcccttc ccacaacaaa tcctgatacc
     4621 tgaagcctga agcattcatc gccccgcgca tttgtggccc ttgtccggtt c
//
LOCUS       DATAIR010000653         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_415011, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000653 DATAIR010000000
VERSION     DATAIR010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415011"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="atpD"
                     /locus_tag="VHM69_15455"
     CDS             complement(<1..1038)
                     /gene="atpD"
                     /locus_tag="VHM69_15455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2741839.1"
                     /translation="MTESGQPFNRTTFGGERGEVDNGHAGTQPEGNGHEERGTGVGTV
                     IEVKGPVVDVRFPPENIPEIFNAVTVPIEADGETTELTLEVQQLLGDDMVRTVAMAST
                     DGLRRGVDAQDTGQPIAVPVGKSVLGRVLDVLGEPVDEQGPVEDAEDYWPIHRPSPKF
                     QDLATQAEQFVTGIKVIDLLCPVRRGGKVGLFGGAGVGKTVVIQELINNIALQYEGTS
                     VFAGVGERTREGNDLYGEFQDAGILPNVGLVFGQMNEPPGARFRVALTGVTIAEYFRE
                     ELGQDVLFFVDNIFRFVQSGNEVSALMGRMPSEVGYQPTLGTEMGALQERITSTKKGS
                     ITSFQAVYVPAD"
     gene            complement(1053..1916)
                     /gene="atpG"
                     /locus_tag="VHM69_15460"
     CDS             complement(1053..1916)
                     /gene="atpG"
                     /locus_tag="VHM69_15460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX2741840.1"
                     /translation="MASARDFQRQINTVKNQARVFSALQTVSSVKFRKAEARVRRARP
                     YAENVEQMMRDVAGSASGDLPLLTGREVQRVAVCTLTADRGLAGGFNAQVLRRTVSLR
                     SEKDPDALQVASGRKAVAFFRFRRVDLAEVFTGFTDNPTYDKAREIGRALTRLFEEEE
                     ADEVFLVYNRFESALNQRPVVKRLLPVAPEDDEEEEERGVSYMEYVPDAETVLKRLVP
                     RYVETMVWQALLEGAAGEHGARMTAMKNATDNANDLVRDLTLQMNKARQAQITQEILE
                     IVGGAEALSAG"
     gene            complement(1920..>2129)
                     /locus_tag="VHM69_15465"
     CDS             complement(1920..>2129)
                     /locus_tag="VHM69_15465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX2741841.1"
                     /translation="WERQFRDYMRNSHSDILDTIRSEQQLSDEVEEKLREAIEHFNSN
                     YEPEHTSLVDVSSGDEGENGEEEGS"
BASE COUNT          326 a          727 c          646 g          430 t
ORIGIN      
        1 gtcggcaggc acgtagacgg cctggaagct cgtgatcgaa cccttcttcg tcgaggtaat
       61 ccgctcctga agcgccccca tctcggtccc gagagtaggc tggtaaccga cctccgatgg
      121 catgcggccc ataagcgccg agacctcgtt gccggactgg acgaatcgga agatgttgtc
      181 cacgaagaag agcacgtcct ggcctagctc ctcgcggaag tactcggcaa tggtgacacc
      241 cgtcagcgct acgcggaagc gggcgcctgg cggctcgttc atctggccga agacgagtcc
      301 cacgttaggg agaatcccgg catcctggaa ctcgccgtag aggtcgttcc cctcgcgcgt
      361 gcgctcgccg acgccggcga agaccgacgt accctcgtac tggagggcga tgttgttgat
      421 cagctcctga atgacgaccg tcttgcctac gccggcgccc ccgaagaggc cgaccttgcc
      481 acctcgcctc accgggcaga gaaggtctat gactttgatg ccggtgacga actgctcggc
      541 ctgcgtcgcg aggtcctgaa acttcgggga cggacggtgt atcggccagt agtcctcagc
      601 gtcctctacg ggcccctgct cgtcgaccgg ctcccccagc acgtcgagca cccgcccgag
      661 cacgctcttg cctacgggca ccgctattgg ctggcccgta tcctgcgcgt ccaccccgcg
      721 cctcagaccg tcggtcgagg ccatcgccac cgtgcggacc atgtcgtccc cgaggagctg
      781 ttgcacctcc agggtcaact cggtcgtctc cccgtcggcc tcgataggca ccgtgacggc
      841 gttgaagatc tccggtatgt tctcaggcgg gaacctgacg tcaacgacgg gccccttgac
      901 ctcgataacg gtgcctacgc ccgtgcctct ttcctcgtgc ccgttgcctt caggctgcgt
      961 acccgcgtgg ccgttatcca cttcgcccct ttcgccgccg aacgtcgtac ggttaaaggg
     1021 ctgtccgctc tcggtcataa aactccttct ctctagcccg cggagagggc ctccgcgccg
     1081 ccgacgatct cgaggatctc ctgcgtgatc tgggcctggc gcgccttgtt catctgtagg
     1141 gtgaggtcac gtaccaggtc gtttgcgttg tccgtagcgt tcttcatcgc cgtcatgcgg
     1201 gccccgtgct cgcccgcggc gccctcgagg agggcctgcc acaccatcgt ctccacgtag
     1261 cggggaacga gcctcttcag gaccgtctcc gcgtcgggga cgtactccat gtaggagacg
     1321 cctctctcct cttcctcctc atcatcctcc ggggcaacgg gcaggaggcg cttgacgacg
     1381 ggcctctggt tcagtgcgct ctcgaagcgg ttgtagacca ggaacacctc gtcggcctct
     1441 tcctcttcga agagccgcgt gagggcgcgc ccgatctcac gcgccttatc gtacgtcggg
     1501 ttgtctgtga agcctgtgaa gacctcggcg agatccaccc gcctgaacct gaagaacgcg
     1561 acggccttgc gcccgctcgc gacctgcagg gcgtcggggt ccttctcgct tctgaggctg
     1621 acggtcctgc ggagcacctg cgcattgaac ccgccggcca ggccgcggtc tgccgtaagg
     1681 gtgcagacgg ccacccgctg tacctcacgg cctgtgagga gcgggaggtc tccggaggcg
     1741 ctgccagcaa cgtcgcgcat catctgctcg acgttctcgg catatgggcg cgcccgcctg
     1801 accctggcct ccgctttgcg gaacttcacc gaggataccg tctgaagggc ggagaatacc
     1861 cttgcctggt tctttacggt gttgatctgc ctctgaaagt ccctggcgga agccaagctc
     1921 tagctcccct cttcctcgcc gttctcccct tcgtccccgg aggagacgtc gacgaggctc
     1981 gtgtgctcgg gctcgtagtt ggagttgaaa tgttctatgg cctcgcgcag cttctcctcg
     2041 acctcgtcgg agagctgctg ctcggagcgg atagtgtcga gaatatcgct gtggctgttg
     2101 cgcatgtagt cgcggaactg gcgctccca
//
LOCUS       DATAIR010000654         8466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_205143, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000654 DATAIR010000000
VERSION     DATAIR010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205143"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VHM69_15470"
     CDS             <1..740
                     /locus_tag="VHM69_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2741842.1"
                     /translation="MGAWDEAEALAIQADGRIVAAGSAESEDGDYDVAIARYHPITDN
                     TPPNTTITSGPSGAVNSTSATFAFVSSEPGSKFQCSLDGATFSACSSPKSYSSLNDGS
                     HLFGVRAIDASGNTDQTPAQRSFTVDTRAPKVNSVLPTEDDTGVSPGVNIKATFSEAI
                     MASSITTDTVKLFRAGTTTPIAAVASYDTGTKTATLIPDNDLRLGSKYKAVVTTATRD
                     LAGNRLDQDQDSDNGNQPKVWKFTTSS"
     gene            complement(1207..2907)
                     /locus_tag="VHM69_15475"
     CDS             complement(1207..2907)
                     /locus_tag="VHM69_15475"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX2741843.1"
                     /translation="MQQQWWKESVVYQIWPRSFADGDGDGIGDLAGIISRLDYLTALG
                     VDVLWLSPIYPSPQDDAGYDISDYQDIDSTFGSLEQFDALLAAVHERGMKLVMDLVVN
                     HTSDEHPWFVESRASKDNPKRDWYWWRPAREGMSPGDPGAEPNNWGSIFSGPAWELDD
                     NTDEYYMHLFSRKQPDLNWENSEVRQEIYSMMRWWLDRGIDGFRMDVINFISKNTDLP
                     DGHVHDASLYGDGMPHFVDGPRIHEFLQEMHREVFAGREGLITVGEMPGVTVEEARLY
                     TDPARRELDMVFQFEHVQVDHGETRWDVHPLRLRDLKAVLGRWQAGLSEVGWNSLYWN
                     NHDQPRVVSRFGDDGEHRTRSAKMLGTVLHLHRGTPYVYQGEELGMTNAPLASIDDFR
                     DIQTLNHYAEAVGTGADPETVLAALRISSRDNARTPMQWDDTENAGFTTGTPWMALNP
                     NHEEINAQAEIADDDSVFHYYRRLIELRHTEPAVAHGDFHMLLADHRQIYAFTRSYGT
                     TELLVLASFSGEPATIEVPDADRWKGAELVIANYPVDAAEDFQHLALRPWEARVYRMS
                     "
     gene            3845..4531
                     /locus_tag="VHM69_15480"
     CDS             3845..4531
                     /locus_tag="VHM69_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2741844.1"
                     /translation="MSRDAWRERDLGWLDVLLVIIFYLLISVLAGSAVGFLSIGLLSI
                     AGQLLILTVSAFAAIASVGGVLVARRHLSAASVGLRRVNARWLLIGAGLGLLGWFLTR
                     GVVLAYFWVTGDTSNPQAGLVNAAQGTPLQFALTLLVAGLAVPFGEELLFRGVLYTWL
                     RRWGVVVAMVVSALIFGLNHGINVVFPATAILGLLLALAYERSGSIWPGVVGHILYNL
                     LVFSAARLLL"
     gene            4811..5749
                     /locus_tag="VHM69_15485"
     CDS             4811..5749
                     /locus_tag="VHM69_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741845.1"
                     /translation="MSDIRTGVRTVLSRSYLASLPERSVRAGAALTGGLVYEASEVVL
                     PVAVRRSRLYQAIVGRLLRITIELVGGVEGVYPAQEMPVRELLVRKSAGNVVELSSFL
                     AVGWSPVWLLAGASDLVGGTKVYLRALVAELRDAGVLAEDADVASFEELLTTLEDTSG
                     VLADTVDVPPLNVSSMRTSWQELQRQAADLPDADSLEKIFADLQLAARREDRSILEIS
                     SMVALGAVRTGISLGNVHIFDYYRRALRTIVEEGLLSFLRRTSTPYVTRAGSHFDPQS
                     STYSERLLRRWADRRSPMGRQTAPPSKAAEEDVARG"
     gene            5742..6746
                     /locus_tag="VHM69_15490"
     CDS             5742..6746
                     /locus_tag="VHM69_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2741846.1"
                     /translation="MDDADGTSAYLILNAAAGSSADLLRRLTSAARERGIRVRILQPG
                     EDARQATLEAADEGAESLAVAGGDGSVAAVAGVAVECGLPLVVVPTGTLNHFARDLGL
                     DLARPLRALDAFASDHHERRVDIGRINGRPFINNVSLGVYAEMLGDPLYRGDKLRVAQ
                     TKLQAVFSDPELRRALRITPPDEIPLESVVAVVVSNNPYEFARWDRFGHRHRLDTGTL
                     QISVLDASTLDDLERLLAGTLLGALEIRPALRHWTSRRLDVGAPGEMVRAGVDGEPVT
                     LEAPLRFSVDPGALRVLVPEGSHTRRQVPPLEAGWQAARTLRRWLRPQAPGRHFTEVL
                     "
     gene            complement(7033..>8466)
                     /locus_tag="VHM69_15495"
     CDS             complement(7033..>8466)
                     /locus_tag="VHM69_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741847.1"
                     /translation="IIGVSTDLTELKKTEELRRSEERFRLLAENARDLIYRYCLKPTR
                     GFEYVSPSASAITGYTPEEYYADPELVYKLVHPGDRHLIDEVLRSPESLITIRWLRKD
                     GGVIWIEQRIKAIYEEAGELVAIEGIARDVTGRKRVEEALRETQEFLGGILDNAPLPI
                     YGVSAEGRIRLANRFFADFVGMPQEKVIGALLEDVFTEEEARQFLENNRRVIEAESPL
                     IEEEWAESPDGRRYFQTIKFPLRGPDMRTVAIGGISLDVTERRRAEEALSETRRAERR
                     RIARDLHDIVFQDLSGALQSLRLTNLQANSSELGLNLQEEIEALGRASSGLRSAIYDL
                     RYEKEQPFVESVESLVKLNREATPERRIQLVVEEGFSGDLSRKESVEILRVLQEALAN
                     TRRHSAAMNVEVGLRTDDETILIEVADDGRGFDLESARAGIGLSAMRERVEALGARID
                     VRSRPGEGTTVSVRVRRRSGTPNPRRI"
BASE COUNT         1584 a         2561 c         2575 g         1746 t
ORIGIN      
        1 agatgggtgc ctgggacgaa gccgaggcgc tcgcgatcca ggcagatggc aggatcgtcg
       61 ctgcgggaag cgcggaaagc gaggacggcg actatgacgt tgccatcgcc cgctaccacc
      121 ccatcaccga caacacccca cccaacacaa ccataaccag cggtccatcg ggtgccgtta
      181 acagcacctc cgccaccttc gcgttcgtct cctcagaacc cggctcgaag ttccagtgca
      241 gccttgatgg tgccaccttc tctgcgtgct cctcgccaaa gagctactca agcctcaacg
      301 acggtagcca cctcttcggc gtccgggcga tagacgcttc gggcaacaca gatcagactc
      361 ccgcacagcg cagcttcacg gtggacacaa gagcgcccaa ggtcaatagt gtccttccca
      421 ccgaggacga cacaggagta tctccgggag taaacatcaa agccaccttc tctgaggcga
      481 taatggcttc ctccatcacc accgacaccg tcaagctctt cagagcgggc accaccaccc
      541 cgatagctgc cgtggctagc tatgacacgg ggacgaagac ggcgacgctc atccccgaca
      601 acgatctccg gctcgggtca aagtacaagg ctgttgtcac taccgcgacg agggacctgg
      661 cgggcaaccg gctcgaccag gaccaagact ctgacaacgg caatcagccc aaggtttgga
      721 agttcaccac tagcagctag aggctaacgg gaaaggcggt gcgaataagg gccgggaact
      781 accccggccc ttccttatgc tcttattcat cgcagaggct acaatgggat tactccgaag
      841 tctacttcca gcattctgta gaaaccgctt gttgttaggc gcggcagtga ctgttcagta
      901 gataggaggg tgctattgac accactctga caccaacgcg agcgcaatac agtgaaacac
      961 ggggcaagcc atagcagaga aagccgctta gaaaggcggg aattgcaacc tcatgcaaac
     1021 ccctgcaacg cacgaatcat cactcgtagc tggagatgag gtcagcggtt ggagtccgct
     1081 cgccggctct tcttgactag tttgtcgccg ctttgccagg gggcaaaccc catacatcct
     1141 ccggaggact tccagcacgt cggttaacaa aggtgagaag gagtggagtt gggctacgca
     1201 tccaatctag ctcattcggt agacgcgcgc ttcccacggc cggagcgcaa ggtgctgaaa
     1261 gtcctctgcg gcgtcgaccg ggtagttggc gatgacgagc tcggcgcctt tccagcggtc
     1321 ggcgtccggg acttcgatgg tcgcgggctc tccggagaag ctggcgagga cgagcaactc
     1381 ggtggtgccg tagcttctgg tgaacgcgta gatttgcctg tggtccgcga gcagcatgtg
     1441 gaagtcaccg tgggcgacgg cgggctcggt gtggcgcagc tcgatcagcc gccggtagta
     1501 gtggaacacc gagtcgtcgt cggcgatctc ggcctgcgcg ttgatctcct cgtggttggg
     1561 gttgagtgcc atccacggtg tcccggtcgt gaaaccagcg ttttccgtat catcccattg
     1621 catcggggtg cgagcgttat cgcggctgct gatgcgcagc gcggccagga cggtttcggg
     1681 gtcagcgcct gtgcctacgg cctcggcgta gtggttgagc gtttggatgt cgcggaagtc
     1741 gtcgatcgag gccaagggcg cgttggtcat gccgagctct tcgccctggt aaacgtaggg
     1801 cgtcccgcgg tgcagatgca gcacggtgcc gagcatcttt gcggagcgcg tgcgatgttc
     1861 tccgtcgtcg ccgaagcgcg atacgacgcg tggctggtcg tggttgttcc agtacaggct
     1921 gttccaaccg acctcggaca ggcctgcctg ccagcgccct aggaccgcct tgaggtcgcg
     1981 caggcgcagc gggtgaacgt cccatcgcgt ttcgccgtgg tcaacttgga cgtgctcgaa
     2041 ctggaagacc atgtcgagct cgcggcgggc ggggtcggtg tagagcctgg cctcttcgac
     2101 tgtgacgccg ggcatctcgc cgacggtgat caaaccttcc cgtccggcga agacttcgcg
     2161 gtgcatctct tgcaggaact cgtggattct gggcccgtcg acgaaatgcg gcatgccgtc
     2221 gccgtagagg ctggcgtcgt gcacgtggcc gtcgggcagg tcggtattct tggagataaa
     2281 gttgatgacg tccatgcgga agccatcgat gccgcggtcc agccaccagc gcatcatgga
     2341 gtagatctcc tggcggacct cggagttctc ccagttgagg tcgggttgct tgcgcgagaa
     2401 caggtgcatg tagtactcgt ccgtgttgtc gtcgagctcc cacgccgggc cggagaatat
     2461 ggaaccccag ttgttcggct cggcgccagg gtcgcccggg ctcatgccct cgcgcgcagg
     2521 ccgccaccag taccagtcgc gtttcggatt gtccttcgag gcgcgcgact cgacgaacca
     2581 cgggtgttcg tcggaggtgt ggttgacgac gaggtccata acgagcttca tcccccgctc
     2641 gtggaccgct gccagcagcg cgtcgaactg ctccagcgag ccgaaagtgg agtcgatgtc
     2701 ctggtagtca ctgatgtcgt agccggcgtc atcctgcggc gacgggtaga tcggcgacag
     2761 ccacaacacg tccactccca gagctgtcag gtagtccagc cgcgagataa ttcctgcgag
     2821 atcgccgatc ccatctccgt cgccgtcagc gaagctgcgg ggccagatct ggtatacaac
     2881 actctctttc caccattgct gctgcatcgt aactagtacc cctcctgtgg tagtaggttt
     2941 gtcaagacaa gatttagcgc gtcaccttta gatgggcgct ggtctgagtg cctctcatgc
     3001 tcggaataac gtagtgttca cgagagcact gtatcaaacg ctcatgtctg ttggcccgag
     3061 gctatgtaga tatttggttc gcagaaaaac agccgcgccc aagactcatt gcgtactgac
     3121 ctattttgac cccgacccgc acgcaattat ctgcgatacc gggcaatcga gagtacagaa
     3181 aaccccgtag ctatgcaata ttcccaaacc cctgcaacac cctatttttc actcctaatg
     3241 agcaagcggt tcgagtccgc gcgtcggctc tctttttgct tgcatatcct gtacttaacc
     3301 aagagccttc agtacgttca ccgaagcgtt gccagtagta ggtctcacca aaactctcag
     3361 cttacagaat tatccggggc ttacacaacc cctctgtggg cctttgagag ttcgtcatag
     3421 gggttgacca atcgagctgg taaatcgcag tggcggaaga acactcgggc gtctcagcgt
     3481 ggcgtagacc ttgactgaga tgctggccgc ttcactcggc tgtggtagac gtagattact
     3541 gaaacgctgg atgccatgga ggcaccagga gttcgagcat tgaccatcac ggaggaggta
     3601 ctggaatgct ccacacccct gatcctggtt aggatcctat gccgaccccg ttggtcagtc
     3661 catgaccctc gaggacggct agatcgggag ggaggcaaaa gtttcgaccc cggttcggtc
     3721 gatgaggtaa gctcgatcag aatgtgtgtt cgaactgaag gtaccttgcc gcgaccctca
     3781 ggctttatcg gtaaggagag tgttcccgat ggaagaacct catccacagg cggagaatcg
     3841 ggttttgtcg cgggacgctt ggagagagcg tgacctcggc tggctcgatg tgctgctggt
     3901 tatcatcttc tatctgctca tctcggtact cgcaggctcg gcagtcggct ttctcagcat
     3961 tggtctcctg tccatcgccg gacagctgct catactcacc gtaagcgcgt tcgcggcgat
     4021 cgccagcgtc gggggcgttt tggtagcacg gaggcacctg agcgccgcct cagtaggctt
     4081 gcggcgcgtc aatgccagat ggctactcat cggggctggc ttggggctgt tgggctggtt
     4141 cctcacccgt ggagtagtcc tggcgtactt ctgggtcact ggagacacca gcaaccctca
     4201 agcggggctg gtgaacgcgg cgcaaggtac tcctttacag ttcgccctca cactgctggt
     4261 tgctggcctg gccgttccct ttggagagga gttgctgttc cgaggtgtac tctatacctg
     4321 gctcagacgc tggggggtcg tggtcgcgat ggtagtcagc gcgctgatct tcggtcttaa
     4381 ccacggtatc aacgtggtgt tccccgcgac ggcgattctg ggattgcttc tggcactcgc
     4441 atacgagcgt agtggttcga tatggccagg tgttgttgga catatcctct ataacctgct
     4501 cgttttctcg gcggcgcgct tgctgctttg agagcgggac cgggatcctt atgcgggact
     4561 ccgatcctcc atgcaccggg ttacctccga gttctctgcc tccaactccc tgcgggagag
     4621 cttatcgcca agctttaccg cagaggagaa agcggttcgg agtttcgggg agatcacaaa
     4681 tcggcccacg ggactagtcg aattgggcca tgcgccccat gtgcctactc gcgggcaggc
     4741 ttatagtacg tgcacaatgg cgagcctatt tgctgccccg aaagcggttg acaacctcag
     4801 gagttcgaca gtgagcgata taagaacagg agtccgaact gtattgtcga ggtcgtacct
     4861 ggcatccctt cccgagcgaa gcgttcgagc gggagccgcg ctgacaggcg ggctggtcta
     4921 cgaggcgagc gaggtggtac ttccggttgc ggttcgcaga tccaggctct accaggcgat
     4981 agtcggacgc ttgttgcgta tcacgatcga gcttgtaggc ggcgtcgagg gcgtctaccc
     5041 ggcccaggag atgcctgtgc gagagctgct ggtacgcaag tcagctggta acgtggtcga
     5101 gctgagcagc ttccttgcgg tcggttggtc gcccgtctgg ctcctggcag gggcttccga
     5161 cctggtcggt ggtacgaagg tgtacctgcg ggcgttggtc gcggagttac gggatgcagg
     5221 agtactcgcg gaggacgcgg acgtggcatc tttcgaggaa ctgttgacca cgctagagga
     5281 cacctccggc gtgctggccg acacggtaga cgtacccccg ttgaacgtct caagcatgcg
     5341 cacctcgtgg caagaactac agcgacaggc ggcggacctg ccggacgccg acagtctaga
     5401 gaagatcttt gccgatctgc aattggcggc acgacgagag gatcgttcga tactggagat
     5461 ctcttccatg gttgcgctcg gcgcggtacg cacgggcatt tcgctgggaa acgttcacat
     5521 cttcgactac taccgccgcg cgcttcggac gatcgtggag gagggtctgc tttcttttct
     5581 gcggcgaacc agcactccct acgttacgcg ggctggttcc catttcgatc cccagtcgag
     5641 cacctactcc gaacgcctgc tacggcgctg ggccgatcga cgctcgccga tgggacgcca
     5701 gacggcgcca ccgtccaagg cggcggaaga agatgtcgcc cgtggatgac gccgatggca
     5761 cctccgccta tctgatcctc aatgccgccg ccggcagctc ggcggaccta ctgaggaggc
     5821 tgaccagtgc tgctcgcgaa cgcgggatcc gggtgcgcat tctgcagccc ggggaggatg
     5881 cgaggcaagc caccctcgag gctgcggatg aaggtgcgga gtcgctcgcc gtagccggcg
     5941 gcgacgggtc ggtggcggcg gtggccgggg tggccgtgga gtgtggcttg cccctagttg
     6001 tggtgcctac gggcacgctc aaccacttcg cccgcgacct cggattggac ctcgcgcgcc
     6061 cgctacgcgc gctcgacgcc ttcgcttcag atcaccacga gcggcgcgtg gacatcgggc
     6121 ggatcaacgg ccgcccgttc ataaataacg tctcgctggg cgtctatgca gagatgttgg
     6181 gagacccact gtataggggt gacaagcttc gcgtggcgca aacgaaactc caggccgtct
     6241 tctccgaccc ggagctccgg cgagcgctgc gtatcacgcc acccgacgag atcccgctcg
     6301 agagcgtcgt cgccgtggtc gtctcgaaca acccgtacga gttcgcacgg tgggacaggt
     6361 tcggccaccg ccaccgcctt gataccggaa cgttacagat ctccgtgctc gacgctagca
     6421 cgctcgatga tctcgaacga ctgctcgcgg gaacattgct cggtgccctt gagatccgac
     6481 ccgctttacg ccattggacg tccaggcgcc tggatgtggg agcccctggc gagatggtgc
     6541 gggcaggggt agacggcgag ccggtcaccc tcgaggcgcc gctgcgcttc tccgtggacc
     6601 ccggtgcgct gcgtgtgctc gtgcctgaag gctcgcacac gcgccggcag gtgcccccgc
     6661 tagaggccgg ctggcaggcc gcccgaaccc tgcgccgctg gttgcgccca caggctcctg
     6721 gtcgacattt caccgaggta ctgtagtaga gattctcatg gagcagcagt tccaggtcct
     6781 cacgacctca cgacctcctg cacctcatct cacagcggct tgcggaagga ttgatcctcc
     6841 cacaagccgg gctacaggtt tcaggaatca ggaaggtctt cccggacggt ggtcgctaag
     6901 gtgcacctat ggggcgaggt gggtcaatac ttatgaactg cgctgtcaga tacaacgatg
     6961 gatctccgta tcgttgaccg agaatgctag aaggcgcgcg tgctgtagcg gcgagtgagg
     7021 ccaacttgaa tgtcatatgc gtcgaggatt tggagtacca ctccttcttc gaaccctaac
     7081 tgaaaccgtc gtaccctcgc ccggccggct cctcacgtcg attcttgcgc cgagagcctc
     7141 gaccctctcg cgcatcgccg aaagccctat gcccgccctg gcggattcaa gatcgaaccc
     7201 ccggccgtcg tcggcgacct ctatcagaat cgtttcgtca tccgtgcgca accccacctc
     7261 gacattcatc gcggcggagt ggcgccgagt gttagcgagg gcctcctgta gtacgcgcaa
     7321 tatctctacg ctctcctttc tggagagatc tccagagaat ccttcctcga cgaccaactg
     7381 tatcctgcgc tccggcgtcg cttctctgtt cagcttcacc aacgattcga cggactccac
     7441 gaacggttgc tccttctcgt acctgaggtc gtagatggcg cttcgcaggc ccgagctcgc
     7501 ccgtccaagg gcttcgatct cttcctgcag gttcagccct aactctgaac tattggcctg
     7561 caggttggtg agccgcaacg actgtagcgc gcccgaaagg tcttggaaca ctatgtcgtg
     7621 caggtccctg gctatcctgc gacgctcggc ccgacgtgtc tccgagagcg cctcctcagc
     7681 ccggcgacgt tcggttacgt cgagcgagat ccctccgata gcaacagtcc tcatatccgg
     7741 gccacgcaat ggaaatttga tcgtctggaa atagcggcgc ccgtcgggcg actccgccca
     7801 ttcttcttct atcaatgggg actccgcctc gattaccctc cggttgttct caaggaactg
     7861 acgcgcctcc tcctccgtga atacatcttc taacaaagcg ccgattacct tctcctgtgg
     7921 catgccgacg aaatccgcaa agaagcggtt ggccaggcgg attcgaccct ccgcggaaac
     7981 gccatagatc ggaagcggtg cattgtccag gatgcccccc aggaactcct gggtctcacg
     8041 cagagcttcc tcgaccctct tgcgcccggt aacatctcgg gcgatgccct cgatagcaac
     8101 cagctcgcct gcctcttcgt agatcgcttt gatgcgctgc tcgatccata tgaccccgcc
     8161 atctttgcga agccagcgga tggtgatgag ggactccgga gagcgcaata cctcgtcgat
     8221 aaggtgcctg tcgccggggt gaacgagctt gtacaccagc tcgggatcgg cgtagtactc
     8281 ctcgggcgtg tagcctgtta tggcgctggc cgaagggctg acgtactcga agccgcgcgt
     8341 gggcttgagg cagtagcggt agatgagatc ccgggcattc tcggccagga gcctgaacct
     8401 ctcctcgcta cgacgcaact cttccgtctt cttgagttcg gtaaggtccg tactaacgcc
     8461 gatgat
//
LOCUS       DATAIR010000655         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_343033, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000655 DATAIR010000000
VERSION     DATAIR010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343033"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VHM69_15500"
     CDS             <1..2324
                     /locus_tag="VHM69_15500"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2741848.1"
                     /translation="PEEVSLRDLGEQRLKDLFRPERIFQLTASGLPSEFPPLRTLGAL
                     LNNLPLQPTPLVGRVREVEEVTGRLRSEQVRLLTLTGPGGTGKTRLALQSAADLLEHF
                     DDGVFFVPLAAITDPELVPSAIAEPLGVRESAEQTLEEGLKSFIRDTRLLLVLDNFEH
                     ILEGAPLVGELVGACPNLKVLATSRIPLRLYGEWEYPVPPLALPDPMVLPPIKVLTQY
                     EAVKLFVERARAVKEDFEVTNESAPAVAEICARLDGLPLAIELAAARIRILPPQMMLQ
                     RLSNRLKFLKGGSRDLPARQQTLRGTIDWSYDLLPEEEKMLFGRLSVFSGGSTLEAIE
                     EICDPEGDMDALEGVDSLLEKSLIRQEEPDREPRFAMLETVQEYASEKLEESGEAGEI
                     KRAHAEFYLALAERANAELRGPDQLEWLQRLDAEHDNMRAALRWELQQGETELMLRLG
                     GALWLFWSVRGHYGEGRRWLEEALAMDGRGPIESRAMALAGVGVLARDQGDFDRAEEA
                     LAEGLEHLGASERSEAKLYLLLSLGNLAVEREDHGRATELFEERLALSREMGHPMALA
                     SSVMSLATVTHKQGDLDRATELYEEGMDLFRERGDKLGLALCLSNLGLVMYSRGDLGR
                     AAKLTEEGVALLRELGAGAHTAVGLCNLGWIALLQNDLDGAAALFEESLVLAWDNGMK
                     PSVLTTLEGYACVAGAKGAAQRAAQLWGAAQALQEAKGIPRDTDWLTEADERISAVRS
                     VLGEQAWEEAQRKGRAMTLEEAVSYALEEEGKG"
     gene            complement(2488..2619)
                     /locus_tag="VHM69_15505"
     CDS             complement(2488..2619)
                     /locus_tag="VHM69_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741849.1"
                     /translation="MPLDYDGNRMNVCEIVDIEDGKIKSATGYFAGPFEAPEWRAQG"
     gene            complement(3443..3868)
                     /locus_tag="VHM69_15510"
     CDS             complement(3443..3868)
                     /locus_tag="VHM69_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741850.1"
                     /translation="MAGTSPGAGIPRAIADAEGSTEESCCEVGGADPADVGWVAVVGP
                     DEVSGDTDGVDAVGASTLLSAGTVAGAGEAATELATGLDAEGDTELAGSGDPAAAAVG
                     VVDTEPVVSCAWLASLWSRPVCPVEAAPSSSELVSGDPS"
     gene            complement(4272..>4991)
                     /locus_tag="VHM69_15515"
     CDS             complement(4272..>4991)
                     /locus_tag="VHM69_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX2741851.1"
                     /translation="EGVSMNCGEGFSRDVGAPIRAALALRRVHRLRKVFESRFEVFAA
                     IDDYLDLLREQRTPLPRDYYEVGRMARAVHRALEASPVPLVPCHNDPWPGNLLDADGR
                     MYLIDWEYSGMNDPMWDLADLSVEAGFGPEQDRTMVEAYCGAAAPQTLYSRLEVYKAM
                     SDLHWSLWGFVQHAKGNPAEDFWSYALGRLERCKARMSSADFRLHVGAVREGDHPRPQ
                     KRIYRTSQELRTPARKVTESG"
BASE COUNT          996 a         1455 c         1634 g          906 t
ORIGIN      
        1 tgcctgagga agtaagcctc agggacctgg gggagcagcg cctcaaggac ctcttcaggc
       61 cggagaggat cttccagctt acggcgtccg gactgccctc tgagttcccg cctctaagga
      121 cgcttggggc gctgctcaac aaccttccgc tccagcccac ccctcttgta ggtagggtgc
      181 gcgaggtcga ggaggtgaca gggcggctgc gcagcgagca ggtgcggctg ctcaccctca
      241 caggtccggg gggtactggc aagacccgcc tcgccctgca gagtgcggca gatctcctgg
      301 agcacttcga cgacggggtg ttcttcgtcc cccttgccgc cattaccgat ccggagctgg
      361 tgccctccgc catcgctgag cctcttgggg tcagggagag cgcagagcaa accctggagg
      421 agggcctgaa gagtttcatt agagacacgc gcctactgct cgtcctggac aacttcgagc
      481 atatcctgga gggggcgcca ctcgtggggg agcttgtggg tgcatgcccc aatctcaagg
      541 tcttggcaac cagccgcata cccctcaggc tctacggtga gtgggagtac cccgtgcctc
      601 cccttgctct gccagatccc atggtcttgc ctcccatcaa ggtactcacc cagtacgagg
      661 ctgtgaagct cttcgtggag agggccaggg ccgtcaagga agacttcgag gtcaccaacg
      721 agagtgcccc tgcagtagcc gagatctgcg cccgcctgga cggcctgcct cttgcgatag
      781 agttggctgc cgcgaggatc cgcatattgc ccccgcagat gatgctacag aggctttcca
      841 accgtctgaa gttcctgaag ggagggtcgc gcgaccttcc cgcccgccag cagactttga
      901 gggggacaat agactggagc tacgatctcc ttcctgagga ggagaagatg ctcttcggga
      961 ggctctccgt attctctggg ggaagcactc tggaggccat agaggagatc tgcgaccccg
     1021 aaggggatat ggacgccctg gagggggtgg actcgctatt agagaagagc cttatacggc
     1081 aggaggagcc tgacagagag ccgcgcttcg cgatgctcga gactgtacaa gagtatgcca
     1141 gcgagaaact agaggagagc ggtgaggccg gggagatcaa gcgagcgcac gccgagttct
     1201 atttggcttt ggcagaacgg gccaatgcgg aactacgggg cccggatcag ctggaatggc
     1261 tccagaggct tgatgccgaa cacgacaaca tgagggctgc tctcaggtgg gaactccagc
     1321 aaggagagac tgaactgatg ctcaggcttg ggggcgctct gtggttgttc tggtccgtgc
     1381 gcggtcacta tggcgagggg agacgctggc tcgaagaggc gctagcaatg gacgggcgtg
     1441 gcccgatcga gtcgagggcg atggcgctgg ccggggtagg agtgctggcg agggatcagg
     1501 gcgacttcga tcgggcagag gaggccctgg cagagggtct cgaacacctc ggagcatcgg
     1561 agagaagcga ggctaagctc tacctactct tatccttggg aaatctggca gtggaaaggg
     1621 aagaccacgg cagggcaacc gagttgttcg aagaaaggct tgcgttgagc cgggaaatgg
     1681 ggcaccctat ggcgctcgcg agctctgtaa tgagcctggc gaccgttact cataagcagg
     1741 gcgacctcga tcgggctacc gagctctatg aagaaggcat ggatctcttc cgggagcgtg
     1801 gcgacaaact tggcctcgct ttgtgcttga gcaacctggg gctcgtgatg tactccaggg
     1861 gtgatctagg gcgggccgcg aagctcacgg aagagggcgt tgcgctcctg cgagagctgg
     1921 gagccggagc acacaccgct gtcggactct gcaacctggg atggatagcc ctgctccaaa
     1981 acgatctcga cggggcggcg gctctcttcg aagagagcct tgttctcgcg tgggacaacg
     2041 gaatgaaacc gagcgtcctc actactctgg agggatacgc gtgcgtagcc ggagcaaagg
     2101 gagcggcgca gcgggcggcg caactgtggg gtgcggcgca ggcgttacaa gaagcaaagg
     2161 gtatccccag agacaccgac tggctcaccg aggctgacga acgcatttcc gccgtgcgct
     2221 cggtcttggg cgaacaggcg tgggaagagg cgcagcggaa gggtcgggcg atgacgcttg
     2281 aggaggccgt ctcctacgcg ctcgaggaag aagggaaggg ctgatccgcc acgcggtgaa
     2341 cctgaatcct ttcggctctt cgcgaggccc gcgtggagac acggccgtgt ggcacccggc
     2401 gggacaacct tatgggagat gttgcatacg gactttcgcg aacactactt tctactcagt
     2461 tgggtgacta gggtccctaa agtctctcta cccctgcgct ctccactcgg gcgcttcgaa
     2521 gggcccggcg aagtaccccg tcgcgctctt gatcttgccg tcctcaatgt cgactatctc
     2581 gcacacgttc atgcggttgc catcgtagtc gagaggcatc tcgacgacag ccaggtcacc
     2641 gtttatctcg tagctagagt cgatcaggtt cagtacgcct ggatagttat agacggttcg
     2701 cgcggtttgc ttgatcgctc atgagtcctc cttccgctca tggcgggcat cgtcggaggt
     2761 tggttgcggg gtcgattatc gacggatgga agagaccgct catctgccag gcggcgtatt
     2821 cttgccgttg cttgggtagc gaactgctca tcttctgcgc aactgctgaa gagcgacctg
     2881 gcagtcctca acgggatggt tttcggttgc gaacacactc cccttctcac ataatggctg
     2941 ctgtacgcgc gaaaatcatt catttggtgt atgcggcgga cgcggcttcg aggttatcct
     3001 tctacggtag tgcgtagtca atgagggggg agggagaatt ggacaggaga gatcatcccc
     3061 gagagcccga acctcgagtt gcatcccgac aggacctgca aacgtcgacg caagatgaac
     3121 cctggaaggg aatagcaggt cagccgcctg ctctcaccgg cgtcgcgacg ggagatgata
     3181 gagaggatgg gctgaacgct gaagcaggat ggctgacctc agtgagttcc gaactccgct
     3241 cgttccttgg catagcggct ctaatggtct tcctcctgcc tgcggtcgta ctcggattga
     3301 cccttttggg aaacgacgcc aaaccggtgc ccataaagac ggccaccgca cgagatggag
     3361 gaagcgcgac caccagccag gccgagcccg agagaggaga gcggagcggg agcgaagagg
     3421 tgagtgatcg cctcctcggt agtcacgagg gatctccaga gacaagctcg gatgacgacg
     3481 gagcggcttc aacggggcag accggacgag accagagtga cgccaaccag gcgcaagata
     3541 caacaggctc cgtgtcgaca acgccgacgg cggccgcagc gggatccccg gaaccggcca
     3601 gctctgtgtc accttctgcg tccagtcctg tggccaattc tgtggccgct tcgccggcac
     3661 cggccaccgt tccggcggat agtagtgtgg aggcgccgac agcgtctact ccatcggtgt
     3721 cgccggatac ctcatctggt cccaccacag caacccagcc gacgtccgcc gggtcggctc
     3781 ccccgacttc gcaacaggat tcctcggtgg atccctcggc gtcagcgatt gcgcggggga
     3841 tgcccgcacc cggcgacgtc ccggccaagg acgttcaaga aggagactct aacgtcagcc
     3901 gcgacgatgc ctccgaatac gcaggagggc ctccagagtg aaggccgagg acgcctgata
     3961 cacgggtaga ccgggcccgc cgtgccgtca ccgggaaagt ccaacttggg gacatctcac
     4021 catggcaacg ccggtcggga agaagaggac ggcccgggga gggggggaga aaagggccgt
     4081 cctagagttg actcatggac ctatcttacg accgcgcggg catcatggca tctgccacat
     4141 ggcatatatt cgtcagatcc tcacgaagac cgtctgtgta cgggaacgaa atgcactggc
     4201 gccggatgct ggagcctatg cgcttcatgg cggtgcggaa gtcgtaagtg gtgacggaga
     4261 gctggtggag actatccgct ctccgttacc ttgcgggcag gagttctcag ctcctggctg
     4321 gtccgataga tgcgcttctg agggcgcgga tggtcgccct cacgcaccgc gcccacgtgc
     4381 agcctgaaat cggcgctgct catccgtgcc ttgcagcgtt cgagtctacc cagagcatag
     4441 ctccagaaat cctccgcggg attgcctttc gcgtgctgga caaagcccca gagggaccag
     4501 tggagatcgc tcatggcttt gtagacctcc agccgcgagt agagcgtctg aggagcggcg
     4561 gcgccgcagt aggcctccac catagtgcgg tcttgctcgg gcccgaaccc ggcctcgacg
     4621 gagagatcgg caagatccca catcggatcg ttcatcccgg aatactccca gtcgatgaga
     4681 tacatgcgcc cgtccgcatc gaggaggttc ccaggccacg ggtcgttgtg gcagggcacg
     4741 aggggaacag gagaagcctc tagcgcccgg tgtaccgctc tcgccatcct ccctacctcg
     4801 tagtaatccc gcggcagcgg cgtccgctgc tcgcgtaaga ggtcgaggta atcgtcgatc
     4861 gcagcgaaga cctcgaagcg ggactcgaaa acctttctca ggcggtgaac ccgcctgagc
     4921 gccagcgcag cccgaatcgg cgcccccaca tcccggctaa agccttctcc gcagttcatg
     4981 gagacccctt c
//
LOCUS       DATAIR010000656         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_460556, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000656 DATAIR010000000
VERSION     DATAIR010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460556"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VHM69_15520"
     CDS             complement(<1..562)
                     /locus_tag="VHM69_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX2741852.1"
                     /translation="MKRRDKGGLSQRVPRGSDAVASGLAVAALALLAGFISLPLLSLV
                     VWTIDEQAWRAMASPTAVDALLLSIRTTVITMLILLVVGTPAAYVLARVNFRGNRVIN
                     TLIDIPVVLPPSAAGIALLLAFGRFGLVGQHLEVLGITLTFTTTAVVMAEIFVAAPFY
                     VRQAATGFGAVNLTVEEAALVDGARRS"
     gene            complement(559..1383)
                     /gene="modA"
                     /locus_tag="VHM69_15525"
     CDS             complement(559..1383)
                     /gene="modA"
                     /locus_tag="VHM69_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2741853.1"
                     /translation="MSTSVHRRVFLALLAALLVSVLAGLTSCGGGSASGEQMGGTLTI
                     LAASSLTDAFGELAKTFEKQNPGTEVRTSFGASSEMLAQIQQGAPADVFASAAEEEMD
                     TAVKDGLVTGEPQVFVKNREVVMVPKANPANIRRFRDVATPGTRLVLAEDAVPAADYA
                     LEILGKANERYGAGFKKDVLSNVVSREADVRASVNRVTVGDADATFGYASDHTPDIRD
                     KVEVVPIPPDLNIVATYPIAALEDAKSPTLARKWVDLVTSEVGQRVLKQWGFEPAA"
     gene            complement(1486..2658)
                     /locus_tag="VHM69_15530"
     CDS             complement(1486..2658)
                     /locus_tag="VHM69_15530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX2741854.1"
                     /translation="MILGVLSLAIAHAGVAGAQSPARTQYQTEEESITTRVPELEARV
                     RDQDAVLRDRIEEISTVGAELEEAQWRSEGAQARVAELKEQTRQLERQISAQDASFRE
                     VRARYRDQARAAYKGQSLEGLAALLDGLFGSGVGDPGVASILLDSRETLAAYEDSQQT
                     LRNTRRQISQKRSDYGAAIEEKQKTAADLSRRKQALDESIARLGARKARTEARLQELK
                     AAERARILNSAAATGWGEASRGYELEIAREDIVARSVDPIPKKEYIKLYRKSAMDYGF
                     GPDWYILAAVGKVESNHGENMGPSSAGAMGPMQFLPSTWESSGVDGNGDGVANILDPE
                     DSIPAAAGYLKDGGAPRDWYRALYSYNHADWYVMKVLAVAEAYRRLAKDERVGPYI"
     gene            complement(2832..3698)
                     /locus_tag="VHM69_15535"
     CDS             complement(2832..3698)
                     /locus_tag="VHM69_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2741855.1"
                     /translation="MGKRGLGRGLSALIATGESVGGLKFEELPVTAIRPNSRQPRSSF
                     PEAGIKELAASIQEVGILQPLVVRSTPAGFELIAGERRLRAAKEAGLDRVPVLIRQAG
                     DNESMELALVENLQRENLSPLETASAYQALMDGFGLTKEQLARRLGKSRAAVTNTLRL
                     MHLPKRIRTLLSDGKLSEGHARALLGLETEEQMIQLAKQVGSDKLSVRRTEELVRDWL
                     ASPDDSSQTVAREASPTPNEFHEASRLIREAVELPVRVKSLRSGGKLEIRFRKTEELD
                     ALVTLLTSQDES"
     gene            complement(3691..>4345)
                     /locus_tag="VHM69_15540"
     CDS             complement(3691..>4345)
                     /locus_tag="VHM69_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2741856.1"
                     /translation="VDMDPQGNATSGLGVSPGSGGCMYDVLLDGKPLQDVAVTSEVDG
                     LEVAPATINLVGAEVELVSSLAREFKLKRALEKLPHESYDRVLLDCPPSLDLLTLNAL
                     TAADEILIPIQCEYYALEGLTQLMQSIRMVREELNPGLKVGGVLLTMFDPRTNLAHQV
                     AEEVRSFFGDRVFQTIVPRNVRLSEAPSFGLPVALYAPKSTGAEAYSAVADEVLSRG"
BASE COUNT          741 a         1469 c         1240 g          895 t
ORIGIN      
        1 tggaccggcg cgctccgtca acgagagcgg cctcctccac cgtcaggtta accgcgccga
       61 aacccgtcgc cgcctgcctc acataaaagg gagccgccac gaagatctcc gccatcacga
      121 ccgcggtggt cgtgaaagtg agcgttatcc cgagaacttc gaggtgctgc cccacgaggc
      181 cgaatctccc gaaagccagg agtagcgcga ttccggccgc gctcggaggc agcaccacgg
      241 ggatgtctat tagcgtgttg atcacacggt tcccccggaa gttcacccgc gccaggacgt
      301 aggccgcggg cgtgcccacc accagcagaa tcagcatcgt tatgaccgta gtgcggatgc
      361 ttaagagcag ggcgtctacc gcggtcgggg aggccatagc ccgccaggct tgctcgtcta
      421 tcgtccagac aaccaggctc agtagcggca ggcttatgaa tcccgccagc agcgcgagcg
      481 cggcaacggc cagaccggag gcgacagcat cgctcccacg gggaacccgc tgagagaggc
      541 ctcctttgtc ccgtctcttc acgccgcggg ctcgaagccc cactgcttca gcacgcgctg
      601 tcccacctcg ctcgtcacga gatccaccca cttcctcgcc agcgtcgggc ttttcgcgtc
      661 ttcgagggcc gctatggggt aggtcgcgac gatgttcagg tcgggtggga tcgggacgac
      721 ctctactttg tctcttatgt ccggcgtatg gtcgcttgca tagccgaagg tcgcgtcggc
      781 gtcccccacg gtcacgcggt tgaccgatgc ccttacgtcg gcctcgcggg agaccacatt
      841 ggagaggacg tccttcttga agccggcccc gtaccgctcg ttcgccttac ccaggatctc
      901 cagggcgtag tcggcggccg gcaccgcatc ctccgccagc acgagcctgg tgccaggcgt
      961 cgcgacatcc cggaaacgcc gtatgtttgc cgggtttgcc ttgggaacca tgacgacctc
     1021 gcggttcttg acgaaaactt gtggttctcc cgtcaccaga ccatccttta ctgcggtgtc
     1081 catctcttcc tccgccgccg aagcgaacac gtcggcgggg gcgccctgct ggatctgggc
     1141 gagcatctcc gaactggcac cgaagctcgt acggacctcg gtccccggat tctgtttctc
     1201 gaaggtcttt gcaagctccc cgaatgcatc agtgagcgag gaagcggcga gtatggtcag
     1261 cgtccctccc atttgctcgc cgctagccga gcctccccca caactcgtga gtcccgcaag
     1321 gactgaaacc aacaacgcag ccaacagggc tagaaagacc cggcgatgta cgcttgtgct
     1381 caccaggagt ccatgctaga gaaaggctca cgcccggtct ttgtacaaag tgcacgaaaa
     1441 ccgacgccgt gctcgtaagc tcctttgact tggttgtgga gaaacctaga tgtagggacc
     1501 gaccctttcg tccttcgcca gacgtctgta ggcctccgcg acggcgagga ctttcattac
     1561 gtaccagtcg gcgtggttgt agctgtaaag ggctctatac cagtcacgcg gcgctccgcc
     1621 gtccttgagg tagcctgctg ccgcgggtat ggagtcttcc gggtccagta tgttcgcgac
     1681 cccgtcgccg ttcccgtcca cgccggagga ctcccacgtc gaaggcagga actgcatcgg
     1741 gcccatcgcg ccggcgctgg aaggccccat gttctcgccg tggttcgact ccaccttccc
     1801 tactgccgcc agtatgtacc agtcaggccc gaacccgtag tccatggctg acttcctgta
     1861 gagctttatg tactcctttt tgggtatcgg atctaccgac ctggctacta tgtcctcgcg
     1921 tgcgatctcg agctcgtacc ctctgctcgc ctctccccag ccggtagccg ccgcgctatt
     1981 aaggatgcgg gctctctccg ccgccttgag ctcctgcagg cgggcttccg ttcgtgcttt
     2041 tctggcgccc agacgggcta tggactcgtc gagcgcctgc ttacgccggc tgaggtccgc
     2101 ggccgtcttc tgcttctcct cgatggcagc accatagtcg cttcgtttct gcgagatctg
     2161 ccgcctcgtg ttcctgagcg tttgctgaga gtcctcgtat gcggccagag tctctcgact
     2221 atcgaggagg atgcttgcca cgccgggatc ccctacccct gaaccgaaca acccgtccag
     2281 cagggcagcc agcccttcca ggctctggcc tttgtaagcg gcgcgggcct gatccctgta
     2341 cctggctcgc acctcgcgga acgacgcatc ctgcgcggat atctgacgtt ccaattgccg
     2401 ggtctgctcc ttgagctccg cgactcgcgc ctgggcaccc tcggagcgcc attgcgcctc
     2461 ctccaactcg gcgcccactg tggagatctc ctcgatcctg tcccgcaaca cggcgtcctg
     2521 atccctgacc ctcgcctcca actcaggtac ccgcgtcgtt atggactctt cctcagtctg
     2581 gtattgcgtt cgagccggac tctgcgcccc ggctaccccc gcatgggcaa tcgccagaga
     2641 tagtacgccc aggatcaccc aagctcttag ccgtcctccc gacaaaccat ctcctccaca
     2701 cgccagacac ttcgagaaag cgcagacagc taagtctata ccaccgcaac tcgtagcggt
     2761 agataccacc acaaacccgg cggtattcta ccttatacgt acaaaattaa ttgtgcctca
     2821 agtgctttag gctaactttc gtcctgggaa gtcaggaggg taaccagagc atcgagctcc
     2881 tctgttttgc ggaagcggat ctcgagtttt ccgccgcttc tgaggctctt caccctgacc
     2941 ggcaattcga cggcttcgcg gatgaggcgc gaggcttcgt ggaactcgtt tggtgtgggg
     3001 ctggcttccc tggccacggt ttggctagaa tcgtcagggc tggcgagcca gtcccggacg
     3061 agttcctcgg ttctcctgac ggagagctta tctgacccca cttgctttgc gagttggatc
     3121 atctgctcct cggtctccag gccaagtagt gcgcgtgcgt gtccttcgga gagctttccg
     3181 tcgctcagga gggtccggat ccgcttcggc agatgcatga gtcgcagggt gttcgtaacg
     3241 gctgccctgc tcttccccag ccggcgcgcg agctgctctt tggtcagccc gaacccatcc
     3301 atgagagcct ggtaggcaga ggcggtctcc agggggctaa ggttctccct ctgcaggttc
     3361 tctacgagcg ccagctccat ggactcgttg tcgccggcct gcctgatgag gacgggcacc
     3421 ctgtccagtc cagcttcctt cgccgcacgc agccttctct ccccggctat gagctcgaag
     3481 ccggccggcg tcgagcgtac gacgagcggc tgcagtatgc cgacttcctg tatggatgcg
     3541 gcaagctcct tgatccccgc ttctgggaag ctgctcctcg gctggcgcga gttggggcgg
     3601 atcgccgtca cgggcagctc ttcgaactta agccccccca cgctctctcc ggtcgctatg
     3661 agggcggaga gtcccctacc cagccctctc ttacccacga ctgagcacct cgtccgcgac
     3721 ggccgaatac gcctcggctc cggtactctt cggcgcgtag agggccacgg gcaacccgaa
     3781 gctgggggct tcactcaacc ttacgttcct cgggacaatg gtctggaaga cgcggtcccc
     3841 gaagaaggaa cgcacttcct cggctacctg atgcgccagg ttcgtgcgtg gatcaaacat
     3901 cgtcaggagg accccgccga ccttgagacc agggttcaac tcctccctga ccatcctgat
     3961 actctgcatg agctgcgtga gtccctccaa cgcgtagtac tcgcactgta tcggtatgag
     4021 gatctcatcc gcagcggtca gcgcgttgag cgtcaagaga tctagggatg gcgggcagtc
     4081 gagcaacacc ctgtcatacg actcgtgcgg cagcttctcc agagcgcgct tgagcttgaa
     4141 ctcccgcgcc aggctggaca ccaattcgac ctccgcccca acgaggttga tcgtggccgg
     4201 cgcgacttcg agtccgtcga cttcgctcgt caccgccacg tcctgcaatg gtttgccatc
     4261 gagcagaacg tcgtacatgc atccacccga gcctggcgac acgccgaggc cgcttgtggc
     4321 atttccctgc gggtccatgt cgact
//
LOCUS       DATAIR010000657         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_66727, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000657 DATAIR010000000
VERSION     DATAIR010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66727"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VHM69_15545"
     CDS             complement(<1..294)
                     /locus_tag="VHM69_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX2741857.1"
                     /translation="MSLGERLHSALDGRLDGYLRTDEASRALWSTDASIYLRKPVGVV
                     VARSEGDVRHALSTARDLGLSITPRGTGTSLAGQATAPGLALDVSEMRRALQID"
     gene            651..1157
                     /locus_tag="VHM69_15550"
     CDS             651..1157
                     /locus_tag="VHM69_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741858.1"
                     /translation="MRKMVLVLASVACTFLLASGVALAYGGGGGGTTYNNIRCDGGKC
                     QGTPKADKMLGTLRRDVIYGLKGDDRMWGRSGRDEVYGFDGNDWATGGDGIDKVGGGD
                     GNDVLLDGTLADNARDKIYGSNGRDRLFTNNNPASVDVVDCGSGHDVAHVDSEDIVHN
                     DCERVDLN"
     gene            complement(1504..2295)
                     /locus_tag="VHM69_15555"
     CDS             complement(1504..2295)
                     /locus_tag="VHM69_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2741859.1"
                     /translation="MPTRGETSGEGSRTAGTVATSSGERVGRWLQVALIAAALLAIPV
                     VIVQESGLGGFWEPLADALDWIIWGVFAANLVIMLAVVPDRRRWLFDNPIDVLIVVLT
                     PPFLPATLKIARVLPVVRLVWLLLLAHRMRGLFSLNGLRYAALIVFTVVVGGGVVFVA
                     VEPDQHLSTWDGLWWATETVTTVAYGDISPTTGLGRIVATVVMTTGIGFVALLTGALA
                     QRFLYSGNEDAVPEAPRSEDETMRKLDDLSEQIAELQRTLDERRR"
     gene            2465..2662
                     /locus_tag="VHM69_15560"
     CDS             2465..2662
                     /locus_tag="VHM69_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2741860.1"
                     /translation="MSFNVRGSFRDPGTKNSWRNRAALNVETIERYAPDLIGLQEAQR
                     GNLGAYGKHLSRYAMVRGPRY"
     gene            2921..>3190
                     /locus_tag="VHM69_15565"
     CDS             2921..>3190
                     /locus_tag="VHM69_15565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2741861.1"
                     /translation="MEISARTGQEPPVIVTGDFNSRPGSPTYSNFTESGFSDTYLAPG
                     NEEGANTFHAFSGARFRDAHPERGPRRIDWILLKDPKGRLRTKSHR"
BASE COUNT          662 a         1002 c          952 g          574 t
ORIGIN      
        1 gtctatctga agcgcccggc gcatctcgga gacgtcgagg gctagcccag gggccgtcgc
       61 ctgccccgcc aggctcgtgc ccgtgccacg cggtgtaatg gagagtccca ggtcacgggc
      121 cgtcgaaagg gcgtgccgca cgtccccctc ggaacgggcg acgacgacgc ccacgggctt
      181 gcggaggtag atggaggcgt ctgtggacca gagggcgcgc gaggcttcgt ccgtgcgcag
      241 gtacccgtcc aatcgtccgt cgagagcgga gtgcaaacgc tcacccaggc tcatgtcctt
      301 ctccccgatc atacccggag tatagtatcc agccgtcctg tccacgaagc aagatggact
      361 ttactcccgg accgagccca ccaccaggcg aacgtagccg atccaccaag gtatctgagc
      421 gcctcgggat gtagcatcgc gtaccggcct ggactcctta tggggagtcc aggcgctttc
      481 tctcattcac cgaagctccc gggaagtagg atcgaaaatg ggacatacgc ccccccaaaa
      541 tgggacacca gtcccattcg ctctgcctta ccgcaaatta ctatgtattc gttgtgtaac
      601 gggttagaga tatgaaacga actctgccct actagagaaa ggtgagtagg atgagaaaga
      661 tggttctggt tctggcatcg gtggcctgta cgttcctgct tgcaagcggg gttgcactcg
      721 cctacggggg cggaggtggc ggaacaacgt acaacaacat ccgctgtgat ggtggcaagt
      781 gccagggcac ccccaaggcc gacaagatgt tgggcacact tcggcgagac gtcatctacg
      841 gcctcaaggg cgacgacagg atgtggggcc gatccggtag agacgaggta tacgggttcg
      901 acggcaacga ttgggccacg ggaggggacg gtatagacaa ggtcggcggc ggcgacggga
      961 acgacgtact tctcgacggc acgctggcag ataacgccag agacaagatc tacggcagca
     1021 acggccgcga caggttgttt accaataaca acccggcttc tgttgacgtt gtcgattgcg
     1081 gaagtggcca cgacgtggct catgtagata gcgaagacat cgttcacaac gactgtgaga
     1141 gggtcgacct caactgaagc ggaggaggct attaacccgc cgtcggcgcc tttgtgccag
     1201 actgcgctga cccttgtaca ctgggggacc gtcggccgac ggtcccccgg tcccttcctt
     1261 gctcgtatca aagcacaaaa gcatccacgc agaaatcatt catttgcggt atgtgccagg
     1321 gccttcctcg gtattacaat tacccaatcg tcaagccgga cgccacctga accttccccc
     1381 tcccaggatt ggcggcccgg cttttctatt ggctgggcct cggtccaggc acccgcaccg
     1441 ttccgggctc acctggccgg gctgctcgtt acgatgagcc cgaccttcgc tctgtcagcg
     1501 cggtcatctg cgtctttcat ccagggttct ctggagctcc gcgatctgct cagacaagtc
     1561 gtctagcttg cgcatggtct cgtcttcact ccggggtgct tcgggaaccg cgtcctcgtt
     1621 cccgctgtaa aggaaacgct gggccaaagc tccggtcagc aacgcgacga agccgatacc
     1681 ggtcgtcatc acgacggtag cgacaatgcg ccccagccct gtcgtgggag agatgtcgcc
     1741 gtaagccacg gtcgttacgg tctcggtagc ccaccagagt ccgtcccagg tgctcaagtg
     1801 ctgatccggc tcgacggcga cgaaaaccac cccgccgccg accacaacgg tgaagacgat
     1861 cagggccgcg tagcgcaacc cgttcaagga aaagaggcca cgcattcggt gggcgagtaa
     1921 caggagccag accaacctca ctacaggcag tacgcgggcg atcttcagcg tcgcgggtag
     1981 gaagggtggg gtgagcacta ctatcaacac gtctatggga ttgtcgaaca accagcgacg
     2041 ccgatccggg acaacggcca gcatgatgac cagattggcg gcgaacaccc cccagatgat
     2101 ccaatccagg gcatcggcca ggggttccca gaagccgccg agtccggact cctgcactat
     2161 cacgacgggg atggcgagca gagcggcagc gatcagcgcc acctggagcc acctgcctac
     2221 ccgctctccg gaggaggtag ccaccgtccc ggccgtgcgg ctaccttcac ccgaggtttc
     2281 acctctcgtt ggcatgctct tagggcctcc cctctccaga atctcacttc cgatatcttg
     2341 cgcttcacac gccctcctct aagataccgg agatgatctg cttacggaga gcgtgagatc
     2401 accgttcatc gaggcaagac gtggatagag agggtttcga ctgaacaagt cttcgctgcg
     2461 ggtgatgagc ttcaacgtga gggggtcgtt ccgcgaccct ggcacgaaga actcatggcg
     2521 taaccgggcc gcgctcaacg tcgagactat agagcgctac gctcctgacc tcatcggtct
     2581 ccaggaggcg cagcgcggca acctcggcgc ttacggcaag catctgtcac gttacgctat
     2641 ggtccgaggc ccgaggtact gaaacgccat cccacacgac ttcaacgcga tcctcttcga
     2701 cccagggcgg ctggggctgc tcgactcggg cggcttctgg ctttccgaga cacctgagcg
     2761 acattccaga agctgggaga cgagggtcgc gcgctgcgca acctgggcgc tattcagcat
     2821 cctgggcacg gaactcgcca tccttcacct gaatacgcac ctcgaccacg tgagctccct
     2881 cgctcgccgg gaaggcagca agctcatcgt cgggagaaca gtggagatct ccgctcgcac
     2941 gggccaggaa cctccggtca tcgtcaccgg tgacttcaac agccggccag gcagcccgac
     3001 ctacagcaac ttcactgaat cgggcttctc ggatacttac ctcgcccccg gcaacgagga
     3061 gggagcgaac accttccacg cgttcagtgg cgcacgcttt cgcgatgccc accccgagcg
     3121 gggaccacgg cgtatagatt ggatcctcct gaaggatccc aaagggcgtc ttagaacgaa
     3181 gtcgcacagg
//
LOCUS       DATAIR010000658         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_343101, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000658 DATAIR010000000
VERSION     DATAIR010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343101"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VHM69_15570"
     CDS             complement(<1..850)
                     /locus_tag="VHM69_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2741862.1"
                     /translation="MSTFTFKARTREGEIVQDQIEGDDRMAVAFELRQQGFLVIDVKE
                     QGVGQMDILEPFKKVKLHDLVVFSRQFATMINAGLPIVRSLYVLSEQTDNPKLAEVIV
                     QVRKDVEAGLALSEALDKHPKVFSRLYTEMVRAGEIGGILDGVLLRIADQLEGDQELR
                     RKVKSAMTYPSIVLILAILAASFMLVFIVPVFAQMFEDLGGTLPLPTRVAMGISDILT
                     SLYGVLLYAGMIGAVIAFLRWKNTEQGRKVWGRVSLRIPFKIGDVIQKVALARFARTL
                     GTLTAAG"
     gene            complement(863..1972)
                     /locus_tag="VHM69_15575"
     CDS             complement(863..1972)
                     /locus_tag="VHM69_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX2741863.1"
                     /translation="MIQTGLNDYLFDAVRMGASDLHITVGLPPMVRVSGQVQALDYEP
                     FTSNVTRELIYDVLSNEQRQRLENDWELDFSYTLPRTARFRVNVYFQKGSLGAAFRTI
                     PHEVKSLGELGLPPAVKDLTEKPRGLVLVTGPTGSGKSTTLASMIDRINETRNEHIMS
                     VEDPIEFLHSHKKCIVNQREVNQDTKSFAQALKHVLRQDPDVILVGEMRDLETISLAV
                     TAAETGHLVFGTLHTQDAPQTVDRIIDVFPSHQQDQIRTQLANALQGVITQTLIPRRG
                     GEGRAVAAEVLVPTPGVRNLIREGKNHQIYSAMQTGGKFGMQTMDASLVDLVRRNVIS
                     REEAEKRSSNPEELRRLAGGYTVGAGTGGYSSFKK"
     gene            complement(2027..>2491)
                     /locus_tag="VHM69_15580"
     CDS             complement(2027..>2491)
                     /locus_tag="VHM69_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2741864.1"
                     /translation="LVLATLHTNDAPAAVTRLVEMGVEPFLTASAVDCVIAQRLARKL
                     CERCKRPVEVDEETLGVLGFPFSLFGEGVIDFHEAVGCDRCGGTGYRGRIGIYEMMVV
                     SGRIKEMILKRVSTDEVSRVAESEGMARLREDGLIKAARGVTTIEEVLRTVV"
BASE COUNT          423 a          799 c          752 g          517 t
ORIGIN      
        1 ctcccgcggc ggtcagggtc ccgagggtcc tcgcgaagcg ggcgagggcg accttctgga
       61 ttacgtcccc tatcttgaag ggtatcctga gcgagaccct cccccagacc ttgcggccct
      121 gctccgtgtt cttccacctg aggaatgcga tgaccgcgcc gatcatcccg gcgtaaagga
      181 gcacgccgta gaggctcgtg aggatgtcgg agatgcccat cgcaacccgc gtcgggagcg
      241 gcaacgtccc gccgaggtcc tcgaacatct gggcgaagac gggaactatg aatacgagca
      301 tgaacgaggc cgccagtatg gccaggatca ggactatcga tggataggtc atcgcgctct
      361 tcaccttgcg cctgagctcc tggtcgccct cgagctggtc ggcaatcctg aggagcacgc
      421 cgtcgaggat gcccccgatc tccccagccc tcaccatctc ggtgtagagg cgggagaaga
      481 ctttggggtg tttgtcgagc gcttcggaga gtgcgaggcc ggcctccacg tccttgcgta
      541 cctgcacgat cacctcggcg agcttcgggt tgtcggtctg ctcggagagg acgtagagcg
      601 agcgcacgat ggggagtccg gcgttgatca tggtcgcgaa ttgcctgctg aagacgacga
      661 ggtcgtgcag cttgactttc ttgaagggtt cgaggatgtc catctggccg acgccctgtt
      721 ccttcacgtc aatgacgagg aatccctgct ggcggagctc gaaggcgacc gccatcctgt
      781 cgtcgccctc gatctgatcc tgaacgatct cgccctctct ggtgcgagcc ttgaaggtga
      841 aggtcgacat tcctcgcccc ccttacttct tgaagctgga atacccgccg gtaccggctc
      901 cgacggtgta accgccggcg aggcgcctta gctcctcggg gttgctcgaa cgcttctcgg
      961 cctcttcccg cgagatcacg ttacgccgca cgagatccac cagggacgcg tccatggtct
     1021 gcatcccgaa cttgccgccc gtctgcatag ccgagtagat ctggtggttc ttgccctccc
     1081 tgatgaggtt cctcacgccg ggcgtaggga cgagtacctc ggccgccacg gccctgcctt
     1141 ccccgccgcg ccgcggtatg agagtctggg tgatgacgcc ctgcagcgcg ttggccagct
     1201 gagtcctgat ctggtcctgc tggtgggatg ggaagacgtc gatgatacgg tcgacggtct
     1261 ggggggcgtc ctgggtgtgt agcgtaccga agacgaggtg ccctgtctcc gccgccgtca
     1321 cagcgagcga gatggtctcg aggtcgcgca tctcaccgac gagtatgacg tcggggtcct
     1381 gcctgagtac gtgcttcaat gcctgggcga agctctttgt gtcctggttg acctcgcgct
     1441 ggttgacgat gcacttctta tggctgtgga ggaactctat cgggtcctcg acgctcatga
     1501 tgtgctcgtt tctggtctcg ttgatcctgt cgatcatgga cgcaagggtg gtcgacttgc
     1561 cgctacccgt aggtccggtc acgagcacga gacctcttgg cttctcggtc agatccttga
     1621 cggcaggggg cagaccgagc tcgcccaggc tcttgacctc gtgcgggatc gtcctgaacg
     1681 ccgcaccgag cgagcctttc tggaagtaaa cgtttaccct gaaacgggcg gtacgcggca
     1741 gggtatagct gaagtccagc tcccaatcgt tctccaggcg ctggcgctgc tcgttggaga
     1801 gtacgtcgta gatcaactct ctggtcacgt tggaggtaaa gggctcgtag tcgagcgcct
     1861 gcacctggcc gctgacacgg accatcggcg gcaaccccac ggttatgtgc aagtcgctcg
     1921 cccccatcct gacggcgtcg aaaaggtaat cgttgagccc ggtctgtatc attcttcctc
     1981 catcctcgtc taacccatgg ttatcggcac gatcatcgaa cggtccctag accacggtgc
     2041 gcaggacctc ctctatggtg gtaacgccac gtgccgcctt tatgagaccg tcctccctga
     2101 gcctggccat gccctcggac tccgccacgc ggcttacctc atcagtcgag acgcgcttga
     2161 ggatcatctc ctttatccgc ccgctcacca ccatcatctc gtatatccca atgcgtcccc
     2221 tgtagcctgt gccaccgcac cggtcgcaac ccacggcctc gtggaaatca atcactccct
     2281 cgccgaagag cgagaagggg aacccaagca cgccgagggt ctcctcatcc acctcaactg
     2341 gccgcttgca cctctcacac agcttccttg ccagcctctg ggcgatgacg cagtcaaccg
     2401 ctgatgccgt caggaacggc tccactccca tctccacgag gcgtgtgacc gcggcggggg
     2461 cgtcgttggt gtgcagcgtg gccagtacga g
//
LOCUS       DATAIR010000659         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_251569, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000659 DATAIR010000000
VERSION     DATAIR010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251569"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VHM69_15585"
     CDS             complement(<1..1374)
                     /locus_tag="VHM69_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741865.1"
                     /translation="MASEGSGTQQSDEQQSQGADGQSQQQPQQNQQEGKQPDSEQNTR
                     YEQNLTEQEKAEMQEELSEIEDNAPVRKNDPRTSVQKKEEYRFAANIPGDKTIPEKEY
                     ADYTLEYDEDDAVMNGKIPDVLLDVPVIKVDEINFELNDLRAKVNLFAKVLDLVELSV
                     GVDAYLGRVKLVIKGVEAQALLKVRLDNVTAILDRVLTTIDRNPQIIEKLVEGVSSAV
                     SDIGEGAGNLVGEGLNSAVEDIGAGAGNLVGEGLNSAVEDIGAGAGDLVGEGLNSAVE
                     DIGAGAGDLVGEGLNSAVEDIGAGAGSAVEDIGAGAGDLVGEGLNSAVEDIGAGAGSA
                     VEDIGSGAGSAVEDIGSGAGHAVGEIGEGAGSAVEDIGSGAGHAVGEIGEGAGSAVED
                     IGSGAGSAVEDIGSGAGHAVGEVGEGAGHAVGEIGEGAGSAVEDVGSGAGHAVGEIGE
                     GAGSAVED"
     gene            complement(1555..2388)
                     /locus_tag="VHM69_15590"
     CDS             complement(1555..2388)
                     /locus_tag="VHM69_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2741866.1"
                     /translation="MKIDDMIEAFAADLSDDPGTSPRTAEFYVADLREFARFLEERDV
                     TDVENVGVGAVMEFRNHLLEGNRKRFTVYRKDAAVRRFLDWIRTATDAELDFDPIEPM
                     HQPLDQQIVFLDDDEVDQLLSFPLNTRDDARDAAVIRLMLDTGVTVSEIRGLLKSDVD
                     LEAAQVHLGGPGSHLAPRVRRLSEEAVGAIRSYLRTRDDETSELIVARAARPVTTSKT
                     LQNAIWKRCDQVGMWRVSPMNLRHTFAMRLLRRGASLNELKEALGVRDTSNIGVYKKF
                     L"
     gene            2465..3067
                     /locus_tag="VHM69_15595"
     CDS             2465..3067
                     /locus_tag="VHM69_15595"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2741867.1"
                     /translation="MLVALTTLLCLVALPILPERETVSAKLRTAEVENTGTLLGENSP
                     ELTAPRKSPPLSSRGGAISGAIKITVPRLGLKDVAVPTGSKQAELDRAGIMRLRGSGL
                     PWIAGSNTFITGHALGFPRTRVPYAFYKLDEMRPGDEIFIEDTDGRRYTFRVYDRLTV
                     EPDDYWVTYPVEGMTVVSLQSCTPIPTFEYRLVVRGELVS"
     gene            complement(3142..3765)
                     /locus_tag="VHM69_15600"
     CDS             complement(3142..3765)
                     /locus_tag="VHM69_15600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741868.1"
                     /translation="MKRGNITGRPHLPTLSYYEQNAPNFVRRTRDLDMEAVYEPFLKL
                     LPEGAHVLDAGCGSGRDSRSLLEKGYEVTAFDASEALVALASEHAGIPVLRMRFDELQ
                     FEESFDGVWACASLLHVPQREMDGAISNLSRSLKPGGVFYASFRYGDGESVVEDGRLF
                     NNYTEHTFRDLLGGHSEIEVVNLWRGEGIHRPEVFWLNVLLRKKQTS"
     gene            complement(3776..4279)
                     /locus_tag="VHM69_15605"
     CDS             complement(3776..4279)
                     /locus_tag="VHM69_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2741869.1"
                     /translation="MSISRRLGRLAQGFVSSLQEDERFRERLRASRERSEALRDAFEA
                     GWRGASEEWRRAEERRAAETAAAEEAAENARNSTSEGRNQGWRATATTFVPRRYPPNV
                     LAAYDRLGLVPGASMDEVDKRRRELVKRFHPDRFSDPEKRVRAERVTSEINAAHDAIE
                     RHILRKR"
     gene            complement(4276..>4791)
                     /locus_tag="VHM69_15610"
     CDS             complement(4276..>4791)
                     /locus_tag="VHM69_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741870.1"
                     /translation="FTVERAAEVIRNLPPEVPRLSAVRIVRGTLEAAGIDIAELESST
                     RARESRLNSEIDLSEGRIQQLKDETEEVIRNLEDEIRKAREARNFGVSEEERKISDAE
                     AGLDNIDMVRDFFGLPQEEDGASPSDPAAGEETQVLEGVDEEDDTQILRRPGPLSDSE
                     DYLDSRENKET"
BASE COUNT          791 a         1581 c         1417 g         1002 t
ORIGIN      
        1 gtcctctaca gcagaaccgg cgccctctcc gatctctcct accgcatgac ccgcgccgga
       61 gccaacgtcc tctaccgcgg agccagcgcc ttcgccgatc tcacctaccg cgtggccagc
      121 gccctctcca acctcgccga ccgcatgacc tgcgcccgaa cctatgtctt ccacagcgga
      181 gccagctccc gaaccaatgt cttctacagc ggaaccggcg ccttcgccga tctcgccgac
      241 tgcgtgaccc gcgccagaac cgatatcctc gacggctgag cctgcacctt cgccgatctc
      301 acctactgcg tggccagcac ctgaacctat atcctcgacg gcggagccag ctcccgaacc
      361 gatgtcttct acagcagaac cggctccagc gccgatatcc tctaccgcgg aattcagtcc
      421 ttcgcccacg agatccccgg caccggcacc gatgtcctcg acggctgaac cagcaccagc
      481 gccgatatct tcgactgccg agttgaggcc ctcgccaaca agatcccctg cacccgcgcc
      541 gatatcctct accgcggaat tcagtccttc gcctaccagg tccccggcac cagctccaat
      601 gtcttccact gcggagttga gtccttcgcc aaccagattg ccagcgccag cgccgatgtc
      661 ctcgactgcc gagttgaggc cctcgccaac aaggttgcca gcgccttctc cgatgtctga
      721 aaccgcggag cttacgccct ctaccagctt ctcgataatc tgcgggttgc ggtctatggt
      781 cgtgaggacc ctgtccagga tggcggtcac gttgtcgagc ctgaccttca gtagggcctg
      841 cgcttcgacg ccctttatca cgagcttgac cctgccgagg taagcgtcga ccccgacgga
      901 gagctccaca agatccagca ccttcgcgaa gaggttgacc ttcgccctga ggtcgttgag
      961 ctcgaagttg atctcatcta ccttgataac cggcacgtcg agcaagacgt cggggatctt
     1021 gccgttcatc acggcatcgt cctcgtcgta ctccagcgtg tagtccgcgt actctttctc
     1081 cgggatcgtc ttgtcgccgg gtatgttggc cgcgaacctg tactcctctt tcttctgaac
     1141 cgaggtgcgg gggtcgttct tccttacagg ggcgttgtcc tcgatctcgc tcaattcctc
     1201 ctgcatttcc gctttttcct gctccgtcag gttctgctcg tatcgagtat tctgctcgga
     1261 gtctggctgc ttcccctcct gctggttttg ctgaggctgt tgctgactct gtccgtcagc
     1321 cccctgcgac tgctgctcgt ccgactgctg agtgcccgaa ccctcggatg ccattgctcg
     1381 tgtctcccgt agatagaaat gatttgtgac gttacttata tgccacgctg taggccagcc
     1441 caaacccccc gtgatccagg ctccggcccg cgaacgtagt accttcctgg ttgcgctcct
     1501 aattagatta gatgcctgac gaccgcttcc ggttacacga agcagtggaa aggatcagag
     1561 gaacttcttg tacaccccta tgttggaggt gtcgcggacg ccgagtgctt ctttgagctc
     1621 gttgaggctg gcgccccggc gcaggagcct catggcgaag gtgtggcgga ggttcatagg
     1681 ggagacgcgc cacatcccga cctggtcaca gcgtttccag atggcgtttt gcaacgtctt
     1741 cgaggtcgtg acgggacggg cggcgcgcgc gacgatgagc tcggacgtct cgtcgtcgcg
     1801 cgtcctcagg tagctgcgga tggcccccac agcctcttcg gagaggcgcc gtacgcgcgg
     1861 ggcgaggtga gagccaggac cgccgaggtg cacctgggcg gcttcgagat ccacgtcgct
     1921 cttgaggaga ccacggatct cgctcaccgt cacgcctgtg tcgagcatga gcctgatgac
     1981 tgcggcatca cgggcgtcgt cgcgggtgtt cagggggaag gagaggagtt ggtccacctc
     2041 gtcatcatcc agaaacacta tctgctggtc gagcggctgg tgcatgggct cgattgggtc
     2101 gaagtccagc tcggcgtccg tcgcggtcct gatccagtcg aggaacctcc tcacggctgc
     2161 gtccttgcgg tagacggtga agcgtttgcg gttgccttcg aggaggtggt tgcggaactc
     2221 catcacggca ccgacgccca cgttctcgac gtcggtcacg tccctctctt cgaggaaacg
     2281 ggcgaactcg cgcaggtcgg ccacgtagaa ctccgccgtt cgggggctcg tgccggggtc
     2341 gtccgagaga tcggcggcga aggcttcgat catgtcgtct atcttcacgt gtaccattct
     2401 aacggtaatt cagcgccgct tggtatactg ccggcgggtg atcggtactc caagagcgtg
     2461 ggtcttgctt gtcgcactta cgaccctgct gtgcctcgta gcccttccca tcctgcccga
     2521 aagagagacg gtctcggcga agctccgcac ggcagaggtc gagaacacgg gcacgctgct
     2581 cggagagaac tccccagagc tcacggctcc gcggaaatct cccccgcttt cctcccgtgg
     2641 gggcgccatc tcgggggcca taaagatcac ggttccgagg ctcggcctga aggacgtggc
     2701 agtgccgacg ggatcgaagc aggccgagct agacagggcg ggcataatgc gcctgcgcgg
     2761 cagcggcctg ccctggatag cgggctccaa caccttcatc acggggcacg ccctcggctt
     2821 cccgaggacc agggtaccct acgccttcta caagctcgac gagatgagac ccggtgatga
     2881 gatctttata gaagatacgg acggacgtcg gtacaccttc agggtctacg accgcctgac
     2941 cgtcgagccc gacgactact gggtgaccta ccccgtggaa ggcatgaccg tcgtctcgct
     3001 ccagagctgt acccccatcc cgaccttcga gtaccgtctc gtagtccgcg gcgaactggt
     3061 ttcttagctt gtcagcacgc cgcccgcagg gcggctctca gcacgctcag gcctggcggc
     3121 cttcagcctt tcttgcgaat cctacgaagt ctgcttctta cgcaagagca cgttgagcca
     3181 gaacacctcc ggacgatgga tgccctctcc gcgccagagg ttcacgacct cgatctccga
     3241 atgccctccg agaaggtctc ggaaggtgtg ctcggtatag ttgttgaaga gtcgtccgtc
     3301 ttcgacgacg ctctccccgt cgccgtacct gaaggatgcg taaaagacgc cgcccggttt
     3361 gagcgaacgg gagagattgg agatggcccc atccatctcg cgctgtggca catgaagcag
     3421 agacgcgcac gcccacacgc cgtcaaaact ttcctcgaac tggagttcgt cgaagcgcat
     3481 acgcaagacg ggtatgccgg cgtgttcgga ggccagggcg accagcgcct cgctggcatc
     3541 gaaggcggtt acctcgtaac ccttctccag taaagagcgg ctatcgcggc ctgatccaca
     3601 cccggcgtcg agcacgtgcg cgccttcggg gagcagcttc aagaaaggtt cgtacaccgc
     3661 ctccatgtcc aggtctcggg tgcgacgtac gaagtttggt gcgttctgct cgtaataaga
     3721 aagcgttgga aggtgcggcc gtccggtaat gtttcctcgt ttcaaacggt tgttcctacc
     3781 gcttgcgaag gatgtggcgt tctatggcgt cgtgggccgc gttgatctcc gaggtgacgc
     3841 gctcggcgcg cacacgcttt tcggggtccg agaaacggtc ggggtggaag cgtttgacca
     3901 gttccctgcg cctcttgtcc acctcgtcca tggatgcgcc ggggaccagc ccgagcctgt
     3961 cgtaggcggc gaggacgttg ggcgggtacc tcctggggac aaacgtggtt gccgtggcac
     4021 gccacccctg gtttcgacct tcagaggtgc tgtttctcgc gttctctgcc gcctcttctg
     4081 ccgcggctgt ttctgctgcg cgtcgttcct cggccctgcg ccactcctcg gaagcacccc
     4141 tccaaccggc ctcgaacgca tctctgaggg cttcgctccg ttcgcggctg gcgcggagcc
     4201 tctctcggaa gcgctcgtct tcttgcaggc tcgagacgaa accctgcgcg aggcgcccca
     4261 gacggcgcga gatgctcacg tttccttgtt ttccctgctg tccaggtagt cttcagagtc
     4321 cgagagcggg cctgggcgac ggaggatctg ggtgtcgtct tcctcgtcga caccctccaa
     4381 gacctgcgtc tcttccccgg cagcggggtc ggaaggtgac gcgccgtcct cctcctgggg
     4441 aaggccgaag aagtcacgca ccatgtcgat gttgtcgagg ccggcctcgg cgtcggagat
     4501 cttcctctcc tcctcggaga cgccgaagtt cctcgcctcg cgcgccttgc ggatctcgtc
     4561 ttcgaggttc ctgatgacct cttccgtctc gtccttgagc tgctggatgc ggccctcgct
     4621 gaggtctatc tcggagttca gcctcgactc cctggccctt gtggaggact cgagttcggc
     4681 gatgtctatg cccgcggcct caagcgtccc ccgcacgatg cgcacggcgg agagccgcgg
     4741 cacctcgggc gggaggttcc tgatgacctc ggcggcccgc tcgacggtga a
//
LOCUS       DATAIR010000660         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_66907, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000660 DATAIR010000000
VERSION     DATAIR010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66907"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..167)
                     /locus_tag="VHM69_15615"
     tRNA            complement(97..167)
                     /locus_tag="VHM69_15615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Gln,seq:ctg)
     gene            complement(217..1167)
                     /locus_tag="VHM69_15620"
     CDS             complement(217..1167)
                     /locus_tag="VHM69_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX2741871.1"
                     /translation="MDRGTFRDRIAVNRRNSVFLIVCFLALGVVLGYVIGYAWIGDPR
                     GAIFGLVLAFVVGSISGGATYFGGDRMMLAASRAHQITHEDAPILFNVVEEMSLAAGL
                     PMPKVYIIEDTAPNAFATGRDPEHASVAATAGLLQKLDRDELQGVMAHEMSHVANYDI
                     RYSMLVGILVGTTVLISDFFLRGLWFGGGRGGRRSEGGGQIQMIMMVVAIVLAILAPL
                     FARLLQLSISRQREFLADATAVRLTRNPKGLADALAKISGDKEVLEVANRATAHLYIV
                     NPVKSFEKRAKGLFSTHPPIQERIDRLRSMETGGIPETVG"
     gene            complement(1355..>1787)
                     /locus_tag="VHM69_15625"
     CDS             complement(1355..>1787)
                     /locus_tag="VHM69_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX2741872.1"
                     /translation="QLKRRYDLIPNLLEGVKKYFRQEQEVLTQVTQARSRAMQPQSPG
                     EQAQSEARLSGAIGNLFAVAENYPDLKSNTVMLDFQEELSSTENKISFARQHYNDSVG
                     EYNTRIQSFPAVIFSRAMGFTEREYFEAQGPERESVTVSYD"
BASE COUNT          334 a          541 c          570 g          342 t
ORIGIN      
        1 actgtgccca tacccccatt ggggtcgagg cgggtccacg agggtccagc gaaaagcccg
       61 aaaaagaaaa cccctcaaaa cgaggctctt ggaaagctgc cggacctgga ttcgaaccag
      121 gactaagtga ttcagagtca ctcgtgctgc cgttacacca tccggcaatg ttgcgaggag
      181 gtattgtaac gtctcacgcc gggtccgtcg aggtcgctag ccgacggttt cgggtatgcc
      241 gccggtctcc atggagcgaa gccggtctat gcgctcctgt atgggcgggt gggtggagaa
      301 gagtcctttg gcccgcttct cgaagctctt taccgggttg acgatgtaca ggtgggccgt
      361 ggcccggttg gcgacttcga ggacttcctt atcgccggag atcttggcga gcgcgtctgc
      421 caggcccttg gggttgcggg tgagcctgac ggctgtcgcg tcggccagga actcgcgctg
      481 gcggctgatg gagagctgga ggagcctcgc gaagagcggg gccaggatgg cgagcacgat
      541 ggccacgacc atcatgatca tctgtatctg gcctccccct tcactacgcc tgccccccct
      601 accgccgccg aaccacagcc cgcgcaggaa gaagtccgag atcaggaccg tggtaccgac
      661 cagaatgccc acgagcatgg agtatcggat gtcgtagttg gcgacgtggc tcatttcgtg
      721 cgccatcacg ccctgcaatt cgtcgcggtc gagtttctgg aggagccccg cggtcgcggc
      781 gacggaggcg tgctcggggt cgcggcccgt ggcgaaggcg ttgggcgcag tgtcctcgat
      841 gatgtacacc ttcggcatcg ggagcccggc cgcgagcgac atctcctcga ccacgttgaa
      901 tagaatgggc gcgtcttcgt gtgtgatctg atgcgcccgc gaagcggcga gcatcatgcg
      961 gtcaccaccg aagtacgtgg cacctcctga gatgctcccg acgacgaagg cgaggacgag
     1021 gccgaaaatc gcgcccctcg ggtcaccgat ccaggcgtac ccgatcacgt acccaagcac
     1081 cacgcccagc gcgaggaagc acactatcag gaagacgctg ttgcgcctgt ttaccgctat
     1141 gcggtctctg aaggttcccc ggtccatcta gcgatcaacc tgtccggtac ggaaacctgg
     1201 cagagatttg gtacttccat gcctctggag tcgggagatc gggagttggg aaaaagcaaa
     1261 actcctactc cctactcccg aagcgagccg taggcgagcg gctcccgact cccgaacgaa
     1321 agagcgatgc gaagtatcca cctggcttcc gccctcaatc gtaggagacg gtgacggact
     1381 cgcgctcggg gccctgggcc tcgaagtatt cccgctcggt gaagcccatc gcgcggctga
     1441 agatgacggc agggaagctc tgtatgcgcg tgttgtactc gccaaccgag tcgttgtagt
     1501 gctgcctggc aaaggagatc ttgttctctg tcgaggacag ctcctcctgg aaatccagca
     1561 tcaccgtgtt gctcttgaga tcggggtagt tctcggcgac cgcgaacagg ttcccgatgg
     1621 ccccggatag cctcgcctcg gactgcgcct gctcgccagg agactgcggc tgcatcgcgc
     1681 gcgaacgcgc ctgcgtcacc tgcgtcagga cttcctgctc ctggcggaaa tacttcttca
     1741 cgccttcgag gaggtttggg atcaggtcgt aacggcgttt gagttgc
//
LOCUS       DATAIR010000661         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_46147, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000661 DATAIR010000000
VERSION     DATAIR010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46147"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VHM69_15630"
     CDS             complement(<1..794)
                     /locus_tag="VHM69_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX2741873.1"
                     /translation="MQDQNINRAEIVTIGTEMLLGDLVDTNTAWISQRLAGLGVGIYR
                     HTTVGDNPERIVDALREAASRSDLVVTTGGLGPTSDDLTNACLSTLTGREMVEYQEAR
                     EHVDRKFAKFGREPTENNYKQALFPKGTELIPNPVGTAMGALVEWEGTLFATLPGVPP
                     EMKTMFGATLEPLIRARSDGSIVSKTLHFAGIGESALAEKVQEFLDATDPTVAPLAGQ
                     GRVRLRITTRAASEKEAQEKIAPVEKEMIARLSDYYYGEGDETLEGS"
     gene            866..1429
                     /gene="yqeK"
                     /locus_tag="VHM69_15635"
     CDS             866..1429
                     /gene="yqeK"
                     /locus_tag="VHM69_15635"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEX2741874.1"
                     /translation="MARSRLSEKRYGHTVRVADTAEDLARAHHVDENRVRLAALLHDA
                     ARETGPEEYLRLADKWEIPVGEPERQSPKLLHGPVAAELARRELGIEDGEVLEAVRAH
                     TTGKPGMGPLALVLYVADKIEPARDYPSVGRLRELAREDLHVVAAESLRRAIAHNEER
                     GRDTHPASLKTLEWLEADHTSQVRVKQ"
     gene            complement(1379..1948)
                     /locus_tag="VHM69_15640"
     CDS             complement(1379..1948)
                     /locus_tag="VHM69_15640"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX2741875.1"
                     /translation="MNATTHAVFGVAALAGAALVAGTDPPAYVYPAAVVAAWLPDVDN
                     PRSTLGNGLSRMKSPVLNLLSRPVSWALRTTSFVLVRTVGHRTLTHSLIGILLFSLPV
                     WLLLGGFPNLFLALVAGYASHVLADALNTRGVPLLWPVGRPFRLLPGGVRSGGAVEVA
                     VALVALAFTLYALLLLYPDLRGVIGLQPL"
BASE COUNT          374 a          667 c          718 g          349 t
ORIGIN      
        1 gagccttcga gggtctcgtc gccctcaccg tagtagtaat cactgaggcg tgcgatcatc
       61 tccttttcga ccggcgctat cttttcctgc gcctccttct cggaggcggc cctggtggtg
      121 atacgcaggc gcaccctgcc ctggccagca agtggggcga ccgtggggtc ggtggcgtcg
      181 aggaattcct ggaccttctc agcgagcgcc gattccccga tgccggcgaa gtggagcgtc
      241 ttcgagacga tagagccgtc gctgcgggcc cggatcaggg gctccagcgt cgccccgaac
      301 atggtcttca tctccggagg cacccctggt agcgtggcga acagggtccc ttcccactcc
      361 accagcgcgc ccatcgccgt ccctacaggg ttcgggatga gctcagtacc cttcgggaag
      421 agggcctgct tgtagttatt ctccgtcggt tcgcggccga acttcgcgaa cttcctgtcc
      481 acgtgttcgc gggcctcctg atactcgacc atctcgcggc ccgtcagggt cgagaggcac
      541 gcgttcgtca ggtcgtccga ggtcgggccg agtccacccg ttgtgacgac caggtcggag
      601 cgggaggcgg cctcgcgcag ggcgtcgacg atcctctccg ggttgtcccc gacggttgtg
      661 tgccggtaga tacccacccc gagtccggcg aggcgctggc tgatccaggc cgtgttggta
      721 tccacgaggt ccccgaggag catctcggtc ccgatggtca cgatctccgc cctgtttatg
      781 ttttgatcct gcacgcgagt attttacaat ccgccggatg gaagacaggg cgaaaactca
      841 cactctcctc gaagaagccg ctgccttggc ccgttctcgg ctctcggaga agcgctacgg
      901 ccataccgta agggtggcgg ataccgcaga agacctggcg cgggcccacc atgtggacga
      961 gaaccgcgtg cggctggccg ccctcctgca cgacgcggcc cgcgaaacgg gaccggagga
     1021 gtatctgagg ctggcagaca aatgggagat acccgtcggc gagccggagc ggcagagtcc
     1081 caagctactg cacggcccgg ttgcggcgga gctggcacgg agggagctcg gcatcgaaga
     1141 cggggaggtt ctggaggcgg ttcgggccca caccacaggg aagcctggga tggggccact
     1201 cgcgctcgtt ttgtacgtgg ctgacaagat cgaacccgcc cgcgactacc cgagcgtggg
     1261 acgtctgcgc gagcttgcca gagaggatct ccacgtagtc gccgccgagt ccctgaggcg
     1321 tgccatagcc cacaacgaag agcgcggacg cgacacgcac cctgcgagcc tcaagactct
     1381 agagtggctg gaggccgatc acacctcgca agtccgggta aagcagtagt agcgcgtaaa
     1441 gcgtgaatgc aagtgccacg agggccacgg cgacctcgac ggctccgccc gaccggacgc
     1501 cgccgggcag cagcctgaac ggcctcccta cgggccacag gagcggcacg cccctggtgt
     1561 tgagcgcgtc ggcgaggacg tgcgaggcgt agccggcgac gagggcgagg aagaggttag
     1621 ggaaacctcc aaggagcagc cagaccggga gcgaaaagag caggatgccg atcagcgagt
     1681 gggtcagggt gcggtggcct acggtcctga cgagcacgaa agaagtcgtc ctcaaagccc
     1741 agctgacggg cctgctcagg agattgagca cggggctctt catcctgctg aggccattgc
     1801 ccaaagtgct gcgcgggttg tctacgtcgg gcagccacgc cgcgacgacg gcagccgggt
     1861 agacgtaggc gggcgggtcg gtgcctgcga cgagcgcggc acccgcaagg gccgcgactc
     1921 cgaatacggc gtgggtggtc gcattcaatc ctggcgcagt ttagcaactc cgcggctcgg
     1981 aggtttcagg catcggcagt cgggcgttcg atcgtcgaac tctgccgggt gggcgccaca
     2041 ccaagctgtg ccgtggcacg tcagtcgttt agcggagcac cgtatctcga acccgtcgcc
     2101 cgaaccat
//
LOCUS       DATAIR010000662         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_67118, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000662 DATAIR010000000
VERSION     DATAIR010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67118"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHM69_15645"
     CDS             <1..220
                     /locus_tag="VHM69_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741876.1"
                     /translation="YVADKIEPARDYPSVGRLRELAREDLHEVAAETLKRAIAHNEER
                     GRDTHPASLKTLEWLEADHTSQVRVEQK"
     gene            complement(167..736)
                     /locus_tag="VHM69_15650"
     CDS             complement(167..736)
                     /locus_tag="VHM69_15650"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX2741877.1"
                     /translation="MNGTTHAVFGVAALAGAALVAGTDPPAYVYPAAVVAAWLPDVDN
                     PRSTLGNGLSRMKSPVLNLLSRPVSWALRTTSFVLVRTVGHRTLTHSLIGILLFSLPI
                     WLLLGGFPNLFLALVAGYASHVLADALNTRGVPLLWPVGRPFRLLPGGVRSGGAVEVA
                     VALVALAFTLYALLLLYPDLRGVIGLQPL"
     gene            1317..1439
                     /locus_tag="VHM69_15655"
     CDS             1317..1439
                     /locus_tag="VHM69_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741878.1"
                     /translation="MSRRTAAWLAYSMLALAVILGVLGCLLFFLNDQPLRGTLG"
     gene            complement(2384..2899)
                     /locus_tag="VHM69_15660"
     CDS             complement(2384..2899)
                     /locus_tag="VHM69_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX2741879.1"
                     /translation="MRFLCDAMLGGLAKWLRAAGYNAYYAREGTDVSDAALTRKALEE
                     SRVLLTSDGGFLEHKPVRDGSFEFLRVPHLPLEDQLRLVVETFGLVRRESRCMECNGE
                     LEIVPCEAVRPLGQVFAGSDGHVVLCVRAVGAEPQGGVSGLHRLPNDSEHLVAQSVEV
                     GLLAQPGTEGG"
     gene            complement(2901..>3457)
                     /gene="dut"
                     /locus_tag="VHM69_15665"
     CDS             complement(2901..>3457)
                     /gene="dut"
                     /locus_tag="VHM69_15665"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX2741880.1"
                     /translation="QTGYSEACVVVYLPNYLMPEASYLKPPGATMLAMDSERSLRVPF
                     RRLVPEAGMPERAHAGDAGYDLRSVEEVDLAPGGRALVRTGISVAIPEGYAGLVLPRS
                     GLALRHGISLVNTPGLIDSGYRGEIQVPLINHDREATFRIEVGTRIAQLVLVRAATAD
                     FTEVQFLESSSDGRGEGGFGSSGR"
BASE COUNT          638 a         1078 c         1111 g          630 t
ORIGIN      
        1 gtacgtggct gacaagatcg aacccgcccg cgactacccg agcgtgggac gcctgcgcga
       61 gcttgccaga gaggatctcc acgaagtcgc cgccgagacc ctgaagcgcg ccatagccca
      121 caatgaagag cgcggacgcg acacgcaccc tgcgagcctc aagactctag agtggctgga
      181 ggccgatcac acctcgcagg tccgggtaga gcagaagtag cgcgtaaaga gtgaaggcaa
      241 gggccacgag ggccacggcg acctcgacgg ctccgcctga ccgtacgcct ccgggcagca
      301 gcctgaacgg cctccctaca ggccacagga gcggcacgcc cctggtgttg agcgcgtcgg
      361 cgaggacgtg cgaggcgtat ccggcgacga gggcgaggaa gaggttaggg aaacctccaa
      421 ggagcagcca gatcggaagc gaaaagagca ggatgccgat cagcgagtgg gtcagggtgc
      481 ggtggccgac ggtcctgacg agcacgaaag aggtcgtcct caaagcccag ctaacgggcc
      541 tgctcaggag gttgagcacg gggctcttca tcctgctgag gccattgccc aaagtgctgc
      601 gcgggttgtc tacgtcgggc agccacgccg cgacgaccgc agcggggtag acgtaggcgg
      661 gcgggtcggt ccctgcgacg agcgcggcgc ccgcaagggc agcgactccg aatacggcgt
      721 gggtggtccc attcaatcct ggcgcagttt agcaactccg ccgccgggag gtttcaggcg
      781 atggttcgat cgtaggacgc tgccgggttg gaggcacacc agcctgtgcc aaggcatgtc
      841 agtcgtttag cgctcgaagc cgtcgcccga accatcgcct gaagcgagtc ctgtgcgaat
      901 cggcgctgca gcctccgggc tagcggatag cccagcttcg ccagaaggta gctcggtcgg
      961 gagaaagcat aaacattgta gaagacagag tcgtcgtcgc gacaccattc cacggtgtga
     1021 tagtgaaaaa catccccgct cagtaccctg gatagccgcc gagctccgcc cagggacggc
     1081 gtgatcccag gactccgcat ctcggccact ctgggagagg agccggaggt cgaagtcgcg
     1141 ctagtctagc ggtgccgtcc tcgtggtccg gccaaggtct tacatgccaa gggtgttctc
     1201 agggtttttc tcggtgcttg ccccgtaacg gttgtcgtaa cgctcaagag acggtactgc
     1261 agtagagtgg tgttgtgacg gaaagggcaa gccaacatgg tcgtgggatc gataggatga
     1321 gccgccgcac cgccgcttgg ttggcctact ccatgctggc gctggccgtg atcctcggcg
     1381 tgctcggctg cctgttgttc ttcctcaacg atcaacccct gagggggaca cttggttaac
     1441 acttgcgttc ctggctttcc ctatggtcgg cgtcgtgatc gtctcccacc gcccgcagaa
     1501 ctccataggc tggatgttct gtttcatcgg tcttgggaac atcctatcgg tcttcgctca
     1561 cgagtacgcg ttctatgcgc tagtcacgca gccaggagct ctgccggggg gcatgtgggt
     1621 gggctggtca ttgttctggg tggcgggcat cacgtggacg ctaatgttcc tttcgcttct
     1681 gctctttccc accggacgac tcccatcgcc tcgttggcgg ccggtcggct gggtgatcgt
     1741 gggcggcatc ttcgtgttga gcgtgctgac agccttcgag tccggtcgga tacaagatat
     1801 acctgtgccc aacccgacag gcatggagca ggcgagcggc atactagcgg tgatccgaat
     1861 catcttgttt cctatccttg tagcgagcat cttcgccgtg gccgcctcgg tcatcgtgcg
     1921 cttccggcgc gcgagaggtg aggagcgcca gcagctcaag tggttcgcct actctgtgag
     1981 cttcttggta ggttcgctgg ggcttggttt gctgaacacg gaggtgctgc ataaccgcgc
     2041 gatcgagtac gccgctggca tctcgggcat cgttgctgtt gtcgcagttc ccacggccat
     2101 cggcgtgggc atcctgcgct accgcctcta cgagatcgac atcatcatca accgcaccct
     2161 cgtctacggc tcgctcacgg cggtgctcgc gctggtgtac ttcgggggcg tgacggcgac
     2221 ccaggcagtt tttacagcgc tcacgggcca aaaagagcag ccgcaactag ccatcgtagt
     2281 ctcaaccctg gtgatagcag cgctgttcac gcccttcaga cgacgcatac agcggttcat
     2341 agatcgccgc ttctaccgca ggaagtacga cgccgcaaag acgctagccg ccttcagttc
     2401 caggttgcgc gaggagaccg acctcgacac tttgggcgac gaggtgctcg gagtcgttcg
     2461 ggagacgatg cagcccgctc acgcctcctt gtggctccgc ccccacagcg cggacgcaaa
     2521 ggacgacgtg cccgtctgac ccagcaaaga cctgcccgag gggccgaacg gcttcgcagg
     2581 gtacgatctc cagctccccg ttgcactcca tgcagcgact ctcgcgcctg acgagcccga
     2641 aggtttcgac aacgagcctg agctggtctt cgagcgggag gtgcggtacc ctgagaaatt
     2701 cgaagctccc gtcacgtacc ggcttgtgtt cgaggaagcc tccatcgctc gtcaggagca
     2761 cacgcgactc ttcgagcgct ttgcgggtaa gcgcggcgtc cgagacgtcc gtcccctccc
     2821 tggcgtagta ggcgttgtat ccggcggcgc gcagccactt cgccagcccg ccgagcatcg
     2881 cgtcacagag gaaccgcacg ctagcgtccc gacgagccaa agcccccctc tccacgaccg
     2941 tcggaggaag actcgaggaa ctgtacttcg gtgaagtctg ccgtggctgc ccgcacgaga
     3001 acgagctgcg cgatcctggt tcccacttcg atcctgaagg tcgcctcgcg gtcgtggttt
     3061 atgaggggga cctggatctc accgcggtag cccgagtcta tgagccctgg cgtgttgacg
     3121 agggagatcc catgcctcaa cgcgaggccg ctgcgcggga ggacgagccc ggcgtacccc
     3181 tcagggatgg cgaccgagat ccctgtccgc accagcgccc gcccgccagg cgcaagatcc
     3241 acctcctcca cggaacgaag gtcgtagcca gcgtccccgg catgggcgcg ctcgggcatc
     3301 cctgcctcgg ggacgagacg gcgaaaaggg acgcgcagtg atcgctctga gtccatcgcg
     3361 agcatagtag caccgggagg tttcaggtat gaggcttcag gcatcaggta gttcgggaga
     3421 tacaccacta cgcaagcctc cgaatagccg gtctgac
//
LOCUS       DATAIR010000663         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_67134, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000663 DATAIR010000000
VERSION     DATAIR010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67134"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..431
                     /locus_tag="VHM69_15670"
     CDS             216..431
                     /locus_tag="VHM69_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741881.1"
                     /translation="MRLSRRNATILLVIGIYMLLTWGTRVFTFIGEFRAGTLVAPGVH
                     FTLVIIGLAIGVYLAYLGIKGRRATRI"
     gene            complement(563..2491)
                     /locus_tag="VHM69_15675"
     CDS             complement(563..2491)
                     /locus_tag="VHM69_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX2741882.1"
                     /translation="MGEGLLGPVDRLQRAFHRSPPAFRRGAAMLVDALIVMESFAVVL
                     LFRFAGNVEPVFWRMFWPFAVFSALAFVLLLNENGVYQSILRYTGIYQGVRVLSATAI
                     AAGGLFIAVFAVGPDGFGIMKYNPVPLSVPLMGSVLAYVQLVAVRLYPRVFYEMSLRE
                     VGRRTRTAIVGTGEAGVALAGHIWRTAASETQVVGFISGSRAEVGRHIEGAPVLGVIE
                     DLEDLIAQQGIDQVIIATPQASREEMDWIWRTSTRASAEVKVMPDLGEFLAEGAIKLR
                     ELQIEDLLGREPVDIDLDALSDYINGNRVLVTGAGGSIGRELSRQISRLGPAKLVLLD
                     RDESGLYYLNTELRREDFYDAEVFVGDVTHAERVSFAFETFRPQLVFHAAAYKHVPMM
                     ELQATEAVVNNVLGTLNVARAAGAYGTSKFINVSTDKAVNPVNVMGATKRLAEMVVKN
                     LSVEYPETVYASVRFGNVLGSRGSVVPTFRQQIEVGGPVTVTHPDMIRYFMTIPEAVS
                     LILQAGAMAEGYGTYVLEMGRPVAITDLARKMIEIMGAPNVQIKFVGLRPGEKLKEEL
                     FEEGEERASTGHQMVFRLSSENMSPPGDSNLSELVDAMVYHARSQETGRALDLLRRAV
                     PNYSAADLPQESRLDYPL"
     gene            complement(2732..3709)
                     /locus_tag="VHM69_15680"
     CDS             complement(2732..3709)
                     /locus_tag="VHM69_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741883.1"
                     /translation="MTAGVVAGGVAFLVAGALVPLLVRFAVGRSLLDVPNLRSSHEVP
                     TPRLGGVAIFLGVLAGVPLLRPDGALPLLAAAALVWAVGLVDDLSSLHFGAKAAAQTL
                     AAAGLLLFYPPTLLSAAPGELAVFAIGVFWIVALSNAFNFMDGIDGFTGGVALVNALF
                     LMPLVGVAGGFLPALIGATAGFLIWNIHPASIFIGDSGAYFLGFSMAAAALYAPALSG
                     REWTPLGFLACVVVFTPYLFDTGYTLIRRLRGGAGKNIFLAHREHIYQRITPTTGMHR
                     RTSNLYYGASVIAGFAALLVSGGGALSMAGVALALVCCALLAALPKIMR"
     gene            complement(3722..3850)
                     /locus_tag="VHM69_15685"
     CDS             complement(3722..3850)
                     /locus_tag="VHM69_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741884.1"
                     /translation="MFSHRDALIVEASLNAGADRLLTEDIQHGLLIEGMRVENPFL"
     gene            complement(4002..>4256)
                     /locus_tag="VHM69_15690"
     CDS             complement(4002..>4256)
                     /locus_tag="VHM69_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741885.1"
                     /translation="AAAALGRSPRIVKLPIEPVRRALVAAERLKLPLPIDSGQVTRLR
                     EDKAYPYDDARRDLDYAPRTFRDGVALEVARLRKLGMLIS"
BASE COUNT          806 a         1357 c         1313 g          780 t
ORIGIN      
        1 gcctggcgca taatctcaac ggcctcgtcc ctgctgaggg cctttacctc gtcaagattg
       61 cgctgcttca actcagccaa gatctttccc tccgaacact atatacacca gatacttgct
      121 gttacaaatg tcgtccaact attacagcta gtataccctt tgcccttttg tgttgctagt
      181 tcggacatgg ccgctctggt acactcacgg acaaggtgag gctctcccga cgcaacgcga
      241 ctattctcct cgtgatcggc atctacatgc tcctcacctg ggggacgagg gtctttacgt
      301 ttattggcga gttccgcgcg ggtacgctgg tcgcgcccgg cgtccacttc actctcgtca
      361 tcatcggcct cgccatcggc gtgtatctgg catatctcgg catcaagggc cgcagggcga
      421 cacgaatata ggtttcaggc ttcaggccac agataggaag tacttccgac gcaacccgaa
      481 cgtgtgacaa acggtcgtag aagcttaagc gacaactgcc atcgagctat gaatgcgaaa
      541 acctgtagcc tgaaacctat ccttacagcg ggtaatccag cctgctctcc tggggaaggt
      601 cggcggcgga gtagtttggt actgcgcggc gcagtaggtc gagggccctc cctgtctcct
      661 ggctgcgggc gtggtagacc atcgcgtcta cgagttcgga gaggttcgag tcgccgggcg
      721 ggctcatgtt ctccgaggag agccggaaga ccatctggtg gccggtcgag gcccgctcct
      781 ctccctcttc gaagagttcc tccttcagct tctcgcccgg gcgcaggccg acgaacttta
      841 tctgcacgtt cggcgcgccc attatctcga tcatcttcct ggccaggtcg gtaatggcga
      901 cgggacggcc catctcgagg acgtaggtac cgtagccttc ggccatagcc cctgcctgca
      961 ggatcaggga gaccgcctcg gggatggtca tgaagtaccg gatcatgtcg gggtgcgtga
     1021 ccgttacggg gccgccaacc tctatctgct ggcggaaggt cggcacgacc gagccgcggc
     1081 tgccgagcac gttcccgaac ctgacggagg cgtagaccgt ctcggggtac tctacagaga
     1141 ggttcttgac gaccatctcg gccaggcgtt tggtcgcgcc catcacgttg acagggttca
     1201 cggccttgtc cgtcgagacg ttgatgaact tgctcgtccc gtaggccccc gcagcgcggg
     1261 cgacgttgag tgtgccgagt acgttgttga ccacggcctc ggtcgcctgc agctccatca
     1321 tggggacgtg cttgtatgcg gcggcgtgga agaccagttg cggcctgaag gtctcgaagg
     1381 cgaagctgac gcgctcggcg tgcgtgacgt cgccgacgaa gacctcggcg tcgtagaagt
     1441 cctcgcgcct gagctccgtg ttcaggtaat agaggccact ctcgtctcgg tcgagcagga
     1501 ccagcttcgc ggggccgagg cgtgagatct ggcgtgatag ctccctgccg atcgaacctc
     1561 ccgcgccggt aaccagcacc cgattgccgt tgatgtagtc ggagagggcg tcgaggtcta
     1621 tgtccacggg ctcgcgtcca aggaggtctt cgatctggag ctcgcgcaac ttgatggcgc
     1681 cttcggccaa aaactccccg aggtcgggca tgaccttcac ctcagcggaa gcgcgggtgg
     1741 aggtgcgcca gatccagtcc atctcctccc tgctggcctg cggcgtggct atgatgacct
     1801 ggtctatccc ttgctgggcg atgagatctt cgaggtcctc gatcacccct aggacaggcg
     1861 cgccttctat gtgcctgccg acctcagcgc gcgagccgct tatgaagccg acgacctgcg
     1921 tctctgaggc ggccgtgcgc cagatgtgcc cggcgagcgc cacgccagcc tctccagtcc
     1981 ccacgatggc cgtccgcgtc cgtcgcccga cttccctcag ggacatctcg tagaagaccc
     2041 gtggatacaa ccgtacggcg acgagctgca cgtaggcgag gacagacccc atcaacggca
     2101 ccgagagcgg gacggggttg tacttcatga tcccgaaccc gtcgggaccc accgcgaaga
     2161 ccgcgataaa cagtccacca gcggctatgg cggtcgcact cagaacccgc acgccctggt
     2221 agatgccggt gtagcggagg atgctctggt agaccccgtt ctcgttcagg agcaagacga
     2281 aagcaagcgc cgagaagacg gcgaaaggcc agaacatgcg ccagaacacc ggctccacgt
     2341 tgcctgcgaa cctgaagagc agcacgacgg cgaacgactc catgactatg agggcgtcga
     2401 cgagcatcgc cgcaccacgc ctgaaggcgg gcggcgagcg atggaaggcc ctctgcaacc
     2461 ggtccaccgg acccagaagc ccctccccca tcctccgcaa aagccacata gtcacatata
     2521 ttagcttttg ttggcggatc tcaccacttt ttgttcagca cgctcaggct gacgccttcg
     2581 cttgtcagca cggcgccttt ggcgcctttc agctcgcttc gggcctggcg gccctcgctt
     2641 tcaggtttca gcttcatccg ctcgggagcc tcgtgtgact gttacaacct ggctgaaatg
     2701 ctgagagccg ccgtcagcct gagcgtgctg cctatctcat tatcttcggc aatgctgcga
     2761 gtagcgcgca acatacaagc gccagggcga ctccggccat tgaaagggcg cctccccctg
     2821 acacgagcag cgcggcgaaa ccggcgatga cgctcgcgcc gtagtagagg ttgctcgtgc
     2881 gcctgtgcat ccccgtggtg ggtgtgatcc gctggtagat gtgttccctg tgcgccagga
     2941 agatgttctt tccggccccg cccctgagac gtcggatcag ggtgtagccc gtgtcgaaga
     3001 ggtaaggcgt gaagaccacg acgcatgcca ggaatcccag aggcgtccac tcccgtcccg
     3061 agagcgctgg agcgtagagc gccgcggcgg ccatactgaa gccaaggaag tacgcaccag
     3121 aatccccgat aaagatagac gccggatgta tgttccagat caggaaccct gccgtagccc
     3181 cgatgagagc cggcaggaaa ccacctgcaa ccccgacgag cggcatgagg aagagcgcgt
     3241 tcacgagagc gacgcccccc gtgaaaccgt caatgccgtc catgaagttg aaggcgttgc
     3301 tgagtgccac gatccagaac acaccgatgg cgaagaccgc gagctccccg ggagcggctg
     3361 agaggagcgt tggcgggtag aagagcagca ggccagcggc ggctagcgtc tgggcggcgg
     3421 ccttggcgcc gaagtgaagg ctcgacaggt cgtctacgag tccgacggcc cagacgaggg
     3481 ccgccgccgc gagcagcggc aaggcaccat cggggcgaag gagaggtact cctgcgagta
     3541 cgcccaggaa tatggcgacg cctccgagcc ggggcgtcgg gacctcgtgc gaactacgga
     3601 ggttcggcac gtcgagcagg ctccgtccga cggcgaacct cacgagcagc ggcacgagcg
     3661 cccccgcgac caggaacgcc acaccaccgg cgaccacgcc ggccgtcaag gctcccgcgt
     3721 cctacaagaa cgggttctca acgcgcattc cctcgataag caggccgtgc tgtatgtcct
     3781 cggtgagcaa cctatcggcg ccggcattga gcgaggcttc gacgatcagg gcatcccggt
     3841 gagagaacaa tgttggctcc tcgctatgta atccagtttc acgggaatgt ctacccgcac
     3901 cggggacagc ctggagaagc cccgaaccct cgcccgcttt gccttctccg tcagaatgtc
     3961 caagccggct cctctcgtgc cacacggttt cagattagca cctacgagat cagcattccg
     4021 agcttgcgca ggcgtgcgac ctcgagtgct acgccgtcgc ggaaggtgcg cggcgcgtag
     4081 tcgaggtcgc gcctggcgtc atcgtaaggg taagccttat cctcccgcag cctggtgacc
     4141 tgaccggagt cgatcgggag cggcaatttc agcctctccg cagcgacgag tgccctgcgc
     4201 actggttcga tcggcagctt gacgatgcgt ggactcctgc ccagggccgc ggccgc
//
LOCUS       DATAIR010000664         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_343824, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000664 DATAIR010000000
VERSION     DATAIR010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343824"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VHM69_15695"
     CDS             <1..247
                     /locus_tag="VHM69_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2741886.1"
                     /translation="RVVYTASLEQIQDESQDPLKIDINTADAEQLDELPEVGPSTAES
                     IVEYRRLNGEFRSVDELEEIPGIGPKTLEKIEPFATV"
     gene            268..1833
                     /locus_tag="VHM69_15700"
     CDS             268..1833
                     /locus_tag="VHM69_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2741887.1"
                     /translation="MDLSPPVRLDLWAILLAISIAVGTITPPLALMILVASSIVATGA
                     LLRRDLLPTEWRLMAVLAPLYVAGGVGIAVLHAATPDPLAELAAMEPGDLILVGRISS
                     PPEQSSFGYRADLRVEHLWYEGREVLRGGGVEVFAGDLSVGVGDTVRVDGEISLPRIG
                     EDGFDYARYLSTKRISALVDATSVRPVGEERGWIGRVHRRTDVALGYGLRPREAAVVR
                     GMVLGDRSFMPEELEDAFQRSGITHVLAISGQHVAILAAVIYFALRLFTIPVGMRAGV
                     TIVLIWLYILVAGAPPSAIRAGVVATFVLAAQLLGRQVSALHFMTTMLAAVLAYNPLL
                     VYSTGFQLSVAAVFGILLLTKPLKSLVERTLLRPFARPPEQLSNLISVSLAAQISTSP
                     IVAATFDQVSVIGVLTNLIAVPLSGPILILGLLGSLAGNVAPLLAYPLNACNGFLVTA
                     LILVAQGASSLPFASITTPGVTPLLVGLFYAGCVPPIVAERVFSSEHKSLWAALLLLW
                     TVIWLVLVSAGSV"
     gene            1843..>2135
                     /locus_tag="VHM69_15705"
     CDS             1843..>2135
                     /locus_tag="VHM69_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741888.1"
                     /translation="MTVYLLLGDDEERKSRGVEKLRGGRAVEGYDAAGTSPETIVSAC
                     NSFSLFGEGPFVVLKNLDAWNAAQKAVVVDYLQDPSPGADLILLGKKLGARERL"
BASE COUNT          371 a          650 c          673 g          441 t
ORIGIN      
        1 cagggtcgta tacacagcct cactggagca gatacaggat gagtcgcagg acccgctcaa
       61 gattgacata aacaccgcgg atgcagagca actcgacgag ttgcccgagg tcggaccatc
      121 gaccgccgag agcatcgtcg agtacaggcg gttgaatggg gagtttagat ctgtggacga
      181 actcgaggag ataccgggga tcgggccgaa gacattagag aagatagaac cgttcgctac
      241 ggtctaagga ggcatcgagg gcctgatttg gatctcagcc ctcccgtcag gctggacctg
      301 tgggccattc tgctcgccat cagtattgcg gtcggaacga taacaccgcc gctcgccctg
      361 atgattcttg tggcgagctc catcgtggcg acaggcgcgt tgttgaggcg ggatctccta
      421 cccaccgaat ggcgactgat ggcggtcctt gcgccattgt acgtcgctgg aggagttggg
      481 atcgccgtac tccacgctgc gacgcccgat ccacttgcgg agcttgccgc gatggagcca
      541 ggcgatctga tactcgtcgg caggatatct tcgccaccgg aacagagcag cttcggatac
      601 agggccgacc tcagggtcga gcacctctgg tacgagggga gagaggtctt gcgtggtggt
      661 ggcgtcgaag tcttcgccgg cgacctcagc gtcggggttg gtgacacggt gagggtggac
      721 ggtgagatct cactcccccg gatcggcgag gacggcttcg actacgcgcg ctacctctcg
      781 acgaagagga taagcgcact cgtcgatgcg accagcgtca gacctgtagg ggaggagcgc
      841 ggttggatcg gccgggtaca ccggcgcacc gacgtggcgc tcggctacgg gctcaggccg
      901 cgggaggcgg ccgtggttcg cggtatggtc ctcggggacc gttcgttcat gcctgaagaa
      961 ctggaggatg ctttccagcg cagtggtatc acgcacgtac tcgcgatctc gggccagcac
     1021 gtggcgatcc tggcggccgt gatctacttc gccctccgcc tctttactat ccctgtcgga
     1081 atgcgcgccg gcgtcacgat tgtcctgatc tggctctaca tcctggtcgc gggtgcccca
     1141 ccgtcagcga tcagggcggg ggtcgtggct acattcgtgc tcgctgcgca actcctcggg
     1201 cgccaggtct cggcgcttca cttcatgacc acgatgctcg cagccgtgct agcgtacaac
     1261 ccgctactcg tctacagcac cggcttccag ctctcagttg cagcggtctt cggtatcctg
     1321 cttctcacca agcccctgaa atctctcgtc gagcgtacgc tgctaaggcc cttcgccaga
     1381 ccgccggagc aactctccaa tctcatctcc gtctctctag cggcacagat ctcgacctcg
     1441 cccatagtcg ctgctacctt cgatcaagtc tccgtcatag gggtgcttac gaacctgata
     1501 gcggtgccgc tttccggtcc gatcctgatc ctcggcctcc tcggctcgct cgcagggaac
     1561 gtcgcgccct tgctcgctta cccgctcaat gcctgtaacg gcttcctggt cactgccctg
     1621 atccttgtgg cacaaggggc ctcctcgctc ccgttcgcgt ccataacgac acctggggtg
     1681 acaccgctgc tcgtgggact gttctacgca ggatgcgtcc ccccgatagt cgccgagcga
     1741 gtcttctcga gtgaacacaa gtctctatgg gccgccttgc tccttctgtg gacggtgatc
     1801 tggctggtac tcgtcagtgc tggtagcgtt tagaatacgt gcatgacggt ctacctcttg
     1861 cttggagacg acgaggagcg aaagtcccgc ggcgtagaga agctgcgtgg tggccgggcg
     1921 gtcgagggtt acgatgcggc agggacgagt ccggagacga ttgtctcggc gtgcaactcg
     1981 ttctcgctct ttggggaggg accgttcgtg gtcttgaaga acctcgacgc ctggaacgcg
     2041 gcccagaagg ccgtggtggt tgattacctg caggaccctt cgccgggtgc ggacctgatc
     2101 ctcctcggca agaagctcgg cgcacgagag aggct
//
LOCUS       DATAIR010000665         6989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_205894, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000665 DATAIR010000000
VERSION     DATAIR010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205894"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VHM69_15710"
     CDS             complement(<1..584)
                     /locus_tag="VHM69_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2741889.1"
                     /translation="MAKGDMHQRGELRWCRKEHLLLRFASDIFTVLEVDGTILYESPA
                     IEEVLGYRPEDLIGQNAFGYIHPEDLERVLDAFAEGTLDAGSSRRVEYRFRHRDGSWR
                     NLEGVGVNLLDEPDIGGIVITSRDISARKRAEEALLMSEERYRAVVEQSAEGIYLLDA
                     ATKRLIESNPALQMMLGYSASELKGMHIYDIAAHS"
     regulatory      808..885
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            895..2592
                     /locus_tag="VHM69_15715"
     CDS             895..2592
                     /locus_tag="VHM69_15715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX2741890.1"
                     /translation="MLRYIGFVILASVVLTTGFLAPQEKAPSDPTRAPSQPPSQPKPA
                     VGLKEALENSSFEPGEILVQTESGAPADTIQSINRRNDASVEESIPHSQVSVVDLPKG
                     LSVAEAIARYRAAPGVKYAEPNYSAAPAALPPTPNDPDFPKMYDLDNRGQYGGTFDAD
                     IDAPEAWNVEKGSADTSVAVIDTGMDISHKDLRDNVWTNPGEVPGNGIDDDGNGYKDD
                     VHGWDFYNDDASVFDNAEQDTHGTHVAGTIAAEGNNTIGVTGIGWQVKVMSLKFIGPN
                     NRSYIDDAVEAINYSVAEGAQVSNNSWGYEFPRNNPSRILQDAIAEADRANQVFVAAA
                     MNGGADFVGDDLDQGSYSIYPANYTNANIISVAATEKTDHLTGFSNYGATSVDLGAPG
                     KDILSTLPGNNYGFGYGTSMATPHVSGAAALLKSQSPQLKAGEIRARILNSVDKNASL
                     EGKTVSGGRLNAAKALGANTSPVILSLKPGSRVRTSKALIGATVRDEETDLTREQITL
                     YLDGRRKVGFTYDQATNRLTYRTTKLASRRHSVKVVTVDNDARQPLTEIRTWSFKVV"
     gene            complement(2620..3585)
                     /locus_tag="VHM69_15720"
     CDS             complement(2620..3585)
                     /locus_tag="VHM69_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741891.1"
                     /translation="MRAERVRTPTILAAALVAAVVVAILLAAPTVMVPAKASRSDVPS
                     MAFTSDRKATAESVFNIYRIDADGHGPAKRLTDIPGSNVMPTWAPDGSEIAFVNSGLP
                     GIPDIYRMDADGSNESSLTSGLMFELEPAWHPGGRRIVYSDADDLFSMSLDAGGTPTG
                     VPTRLTKNAGVDRQPTVSPNGKRIAFSSDRDGDYDIYVMKAAPESSTNQPVKLTRNTR
                     PDTLPDWSPAGGMLAFSTGNEGARDIYKIKTDFQNISPAANLVGDAADDSDPAWSPDG
                     RKVAFISDRSGDSEIWVVGADGTDPSNITKNPASEELQPDWQPLQ"
     gene            complement(3610..5325)
                     /locus_tag="VHM69_15725"
     CDS             complement(3610..5325)
                     /locus_tag="VHM69_15725"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX2741892.1"
                     /translation="MSTNLKDSPAPGWWRAKVIEVFGADLRSLALFRIVVALLVLADL
                     ANRATDLTAHYTDAGVLPRTALVEQVLSPWAFSLNLMNGQLFFQALLFCVAALAAIGL
                     LFGYRTRLMTVVVWVLLISIQWRNPLVTGADGPFLRLLLFWGMFLPLGAYWSVDRALK
                     DGASRLSTRFLSLATVGLFMQIAFVYWFTATLKSGPEWRVDGTALYYALSLDQITTPF
                     GHYLLNFPDLLTVMTFATFGLEALGPFLLLCPFFTGPVRTGAVFAFMSLHFGIWLSMD
                     IGIFPWISALCMVCFLPAWFWDVPFAKLRAALPEWFENAALRMQHAASHMVRDRLSPL
                     HLRLAPAMPAAAVPARQEEGSKLGSRIARIASLPRTLISGEPRDEFAAGQGRTPAGSS
                     EPRMLRSSLVVNLLAFIFLMYVLCWNLTTVSAFKMPEPLYPVGPFLGLDQYWGMFAPS
                     PSREDGWYVIPGNLRDGQQTDLMSITRDDYGMHGVSWEKPQDVNGTFKNEHWRKYLEH
                     IYMQKYSDERLYLGQYICRQWNARHTGAEQLTTFDITYMREMTQPDYKHSTPEKVVLW
                     EHSCV"
     gene            5447..6166
                     /locus_tag="VHM69_15730"
     CDS             5447..6166
                     /locus_tag="VHM69_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741893.1"
                     /translation="MPPTSSRATTGRYANPPVSGSGAAEALAVALGEALAVALAVGEA
                     VALAVGVAVAVADEALAVAVAVGVALADSVVTMPVPPVVPPVVAPVVPTIPVPPVVAP
                     VPAVVPPVVVPLVVGLLVVPLVVGLLVVPLVVGLLVVPLVVGLLVVPLVVGLLVVPLV
                     VGLLVVPLVVGLLVVPLVVGLLVVPPLGPQCGFLFTALPLEVQPTTLPLLFSVLSPSA
                     RPQGSLRSALEPPLQPMPPPD"
     gene            complement(6587..>6989)
                     /locus_tag="VHM69_15735"
     CDS             complement(6587..>6989)
                     /locus_tag="VHM69_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741894.1"
                     /translation="DGRRVISLPASVASRDRGEEPTQEEWRALCRGLLLRLSAQSAEC
                     ERLSIHAAGLEARVEAAEAERDQANAIAEDLYDFFRRRVREVVDRRTESEERTHVEQR
                     SHEEFSGSLGAATTSWMLNASQDGSDRGGGM"
BASE COUNT         1303 a         2152 c         2236 g         1298 t
ORIGIN      
        1 gagtgggctg ctatatcgta gatgtgcata cccttgagct ctgaagcgga gtagccgagc
       61 atcatctgca gagctgggtt ggactcgatg aggcgcttgg tagcagcgtc gaggaggtag
      121 ataccctcag cggactgctc gacgacagcc ctgtagcgtt cctcgctcat caggagcgcc
      181 tcttccgccc gcttgcgcgc gctgatgtcg cgggaggtga tgacgattcc cccgatgtct
      241 ggctcgtcga gcaggttgac gccgacgcct tcgaggttac gccaggagcc gtccctgtgg
      301 cggaaccggt actccacccg ccgcgaggac ccggcatcca atgtgccttc ggcgaaggcg
      361 tccaggaccc tctcgaggtc ttcgggatga atgtagccga aggcgttctg cccgataagg
      421 tcctccggcc ggtagccgag cacctcctct atcgcggggc tctcgtagag gatcgtaccg
      481 tccacctcga gcacggtgaa gatgtcagaa gcaaagcgaa ggagtagatg ctccttcctg
      541 caccagcgta gctcgccacg ctggtgcatg tctccttttg ccatcctcac gctcccggtg
      601 tcttacagag gccggacaga tcggtgaacc cgcggtggat cacacgggac tctgaccggc
      661 acccttgtcg tcgggcggat tatacctgca agcagcggcc tccggacacg acgccagagc
      721 gaagcctgaa aggaacgact ggcatatacg gcggttaacc tatacaatcc gcccggaaag
      781 ggcaaagcgg ccgcaggcgc ggtcctcgcc gcgaaaaggc aaccccgtcg cgaggcgggt
      841 gcgcaaagcc tacgggcctc tcatggaggc ggccgggctg ccgaacgggg gaatatgctt
      901 cgctacattg gttttgtgat cctcgcttct gtcgttctga cgacgggctt tctggcacca
      961 caagaaaagg ctccatccga tccgacgcgg gcgccgtcgc agccgccatc gcagcccaaa
     1021 cccgcggtcg gcctgaagga agcactggag aactcgagct tcgagcccgg cgagatcctt
     1081 gtccagaccg agagcggggc gcctgcggac accatccagt ccatcaaccg caggaacgac
     1141 gcaagcgttg aagaaagcat ccctcactct caggtaagcg tggtggatct ccccaagggg
     1201 ctctcggtcg ccgaggccat cgcgcgttac cgggccgccc caggggtcaa gtacgcggag
     1261 cccaactaca gcgccgctcc cgcggcgctc ccgccgacgc ccaacgaccc tgacttcccg
     1321 aagatgtacg acctggacaa cagaggacag tacggtggca ccttcgatgc ggatatagac
     1381 gcgccggagg cgtggaacgt cgagaagggg agtgccgaca cctccgtggc cgtcatagac
     1441 acggggatgg acataagcca taaggacctc agagacaacg tgtggacgaa cccgggtgag
     1501 gtgcccggca acggcataga cgacgacggt aacggttata aggacgacgt acacgggtgg
     1561 gatttctaca atgacgacgc ctcggtcttc gacaatgccg agcaggatac tcacggtacc
     1621 catgtggccg gtaccattgc cgcagagggg aacaacacca ttggcgtaac cggcatcggc
     1681 tggcaggtca aggtcatgtc gctcaagttc ataggcccca acaacaggag ctacattgac
     1741 gacgccgttg aggccatcaa ctactcggtc gcggaaggcg cccaggtcag caacaactcc
     1801 tggggctacg agttcccgcg gaacaacccg agcaggatcc tccaggatgc catcgctgaa
     1861 gcggaccggg ccaaccaggt cttcgtcgcc gcagcgatga atggaggcgc ggacttcgtc
     1921 ggtgatgacc tcgaccaggg ctcctactcc atctatccgg caaactacac caatgccaac
     1981 atcatctcgg tagcggccac cgagaagacg gatcatctaa cgggcttctc gaactacggc
     2041 gccacttcgg tggacctcgg ggccccgggg aaagacatac tcagtacgct gccgggaaat
     2101 aattacggct ttggctacgg cacttcgatg gctacaccgc acgtatccgg cgccgcggcc
     2161 ctactcaaga gccagtctcc ccagttgaag gcaggcgaaa taagggcacg catcctcaat
     2221 tcggtggaca agaatgccag cctggagggc aagacggtca gcgggggccg cctcaacgcg
     2281 gccaaagccc tcggggccaa cacgtccccg gtcattctca gcctgaaacc tggctccagg
     2341 gtccggacca gcaaagcgct gataggcgcg acggtgcggg acgaggagac ggaccttacc
     2401 agggagcaga taacgctgta tcttgatgga aggcggaaag tcggctttac ctacgatcag
     2461 gctaccaaca ggctcaccta caggaccacg aagctcgcct ctcgcaggca cagcgtgaag
     2521 gtagtgaccg tggacaacga cgcgcggcag ccgctgacgg agatccgcac ctggagcttc
     2581 aaggtagtct gagtcgagga aggtccctgg gtcagggcgt cactgcagcg gttgccagtc
     2641 cggctgcagc tcttctgagg ccgggttctt ggtgatgttg gagggatcgg tgccgtcggc
     2701 cccgaccacc cagatctcgc tgtcgccgct acggtcgctg ataaatgcga ccttccgtcc
     2761 gtcgggagac catgcgggat ccgagtcgtc cgcggcgtca ccgaccaggt ttgccgcggg
     2821 gctgatattc tggaagtcgg tctttatctt gtagatgtcc ctggcacctt cattgcccgt
     2881 ggagaaggcg agcataccgc ctgcgggtga ccagtccggc aaggtatcgg gcctggtgtt
     2941 cctggtgagc ttcacgggtt ggtttgtgga actctctggg gctgccttca tcacgtagat
     3001 gtcatagtct ccgtccctgt cgctggagaa ggctatcctc ttcccgttgg gagagacggt
     3061 gggttgacgg tctacgcccg cgttcttcgt tagccgggtc ggaacgcccg taggagttcc
     3121 gccggcgtcg agggacatcg agaagagatc gtcggcgtca ctgtagacga tcctccgtcc
     3181 gccggggtgc caggcgggtt cgagctcgaa catgaggccg ctggtgagtg aggactcgtt
     3241 ggacccgtcg gcatccatcc tgtagatgtc gggtatgccg gggagacccg agttgacgaa
     3301 cgcgatctcg gaaccgtcgg gcgcccaagt cggcatcacg ttgctccccg ggatgtccgt
     3361 gagccttttc gccgggccgt gcccgtcggc gtcgatacga tagatgttaa acacgctttc
     3421 cgcggtggcc ttacggtcgc tggtgaacgc catgctgggg acgtcgctgc gcgacgcctt
     3481 cgcaggcacc atcaccgtgg gcgccgcaag caaaatagcg accacgacgg ctgctaccag
     3541 ggccgccgcc agtatcgtcg gcgttctcac gcgctcggcc ctcacagtcc aacttccaag
     3601 gtcgcggtcc taaacacaac tgtgctccca caggacgacc ttctcaggtg tggagtgctt
     3661 gtagtccggc tgcgtcatct cccgcatata ggtgatgtcg aatgtcgtga gttgctccgc
     3721 gcccgtgtgg cgcgcgttcc actggcggca gatgtactgg cctaggtaca ggcgctcgtc
     3781 ggagtatttc tgcatgtata tgtgctccag atactttcgc cagtgctcgt tcttgaaggt
     3841 gccgttgacg tcctgcggtt tctcccacga cactccgtgc atgccgtaat cgtcgcgggt
     3901 gatggacatg aggtcggtct gttgcccgtc gcgcaggttc cctgggatga cgtaccagcc
     3961 gtcttccctg ctcggggacg gggcgaacat gccccagtac tggtcgaggc cgaggaaggg
     4021 tccgacggga tagagtggct cgggcatctt gaaggcggag acggtcgtca ggttccagca
     4081 gagtacgtac atcaagaaga tgaaggcgag caggttcacc acgagcgacg accgtaacat
     4141 ccttggctcc gacgaccccg ccggggtcct cccctgtcct gccgcgaact cgtctcgcgg
     4201 ctcgcctgag atcagcgttc tcgggagcga ggcgatgcgg gcgatgcggc tacctagctt
     4261 gctgccttcc tcctgtcgcg cagggacggc cgcggcgggc atggcgggtg ccaggcgcag
     4321 atgtaggggg gagaggcgat ctcgtaccat gtgggacgcc gcgtgttgca tgcggagcgc
     4381 agcattctcg aaccactctg gcaacgccgc cctgagcttc gcgaagggta cgtcccagaa
     4441 ccaggcgggg aggaagcaga ccatacataa cgcgctgatc caggggaaga tcccgatatc
     4501 catcgagagc cagatgccga agtggaggct catgaaggcg aagacggcgc cggtacgcac
     4561 gggccctgtg aagaacgggc acaagagcag gaagggcccg agtgcttcga ggccgaacgt
     4621 ggcgaaggtc atcaccgtga gcaggtcggg gaagttgagc aggtaatggc cgaaaggagt
     4681 ggtgatctga tctaagctca gcgcgtagta gagcgcggtg ccgtcgacgc gccattccgg
     4741 cccggacttc agggtcgcgg tgaaccagta cacgaaggct atctgcatga aaagaccgac
     4801 ggtcgcaagc gacaggaagc gcgtcgagag acgcgatgcc ccgtccttga gcgctcggtc
     4861 gaccgaccag tacgccccga gcggtaggaa catcccccag aagagaagca gccgcaagaa
     4921 tgggccgtcc gctccggtaa cgagcgggtt gcgccactgg atcgagatca aaaggaccca
     4981 cacgacgacg gtcatgaggc gcgtgcggta gccgaagagc aacccgatcg ccgccaacgc
     5041 cgccacacag aacagcagag cctggaagaa tagctgcccg ttcatgaggt tgagcgagaa
     5101 cgcccacggg ctcaagacct gttctacgag agcggtgcgc ggcaataccc ctgcgtcggt
     5161 gtaatgggcg gtgaggtctg tggcccggtt ggccaggtcg gcgagcacca gcagcgccac
     5221 aacgatacgg aacagcgcga gagagcgcag gtccgcaccg aatacctcta taactttcgc
     5281 gcgccaccag cccggtgccg gcgagtcctt gagattggtg ctcatgcgag agttcccctg
     5341 ccgttcacca acgaagaagg aagatagggg gatcgatccc cctatcttcg atgaactcgc
     5401 ctactagcct tgctggcgcc tcagcgctat gagcgagagg agccccgtgc ctccgacgag
     5461 tagcagagcc actaccggca ggtatgcgaa cccgccggtg tcaggaagcg gtgccgccga
     5521 agcgctggca gttgcgctcg gcgaggcgct ggccgttgca ctcgcggtcg gcgaggcggt
     5581 agcactcgca gtcggcgtag cagttgcagt tgccgacgag gcactggcgg tagcagtcgc
     5641 ggtcggcgtg gcacttgccg actcggtcgt aaccatgccc gtgccgccgg tcgtgccgcc
     5701 ggtcgtggca ccggtggtgc caactattcc ggtcccgccc gtagtagcgc cggtgccagc
     5761 agtcgtgccg ccagtggtcg tgccgctggt agtcgggttg ctggttgtgc cactggtggt
     5821 cgggttactg gtcgtaccgc tagtggttgg attgctcgtt gttccgctgg ttgtcggatt
     5881 actagtcgtg ccactagtgg tcggattgct tgtagtccca ctggtagtcg ggttactggt
     5941 cgtgccgcta gtggttggat tgctcgttgt tccgctggtt gtcgggttgc ttgtcgtgcc
     6001 gccgctggga ccgcagtgcg ggttcttgtt cacggcgtta cccttggagg tacagcctac
     6061 gacgttaccc ttgttgttca gcgtgttgtc gccttccgca cgtccgcaag gttcgttacg
     6121 atcggcgttg gaaccgccct tgcagccgat gccgccgccg gattgaccct ggttgttgtt
     6181 gggatcgttg cggtcgttac acgcgctgcc ctcggaaggg ttcccggtca cacatccgcg
     6241 gttggggtcg ttacctccac cgccgccccc gccaccgccg ttgcccccgc caggatctgc
     6301 gggaggccca ccgccgttgc cgccaccgcc gccattgtcg gcgggaggtc ccggagtgcc
     6361 accttttgcc gcatcagcgg gagatccgaa tgcgccaaac gtcagcgcca ggacgacggc
     6421 aagcaccgcc cccagggcga gcatggccgc gaacccgtgc cacctgtgta aagcttccct
     6481 catctagtta cctccttcgt aatgtcgcct ggtccgtgat ttggtggcta gtttcgatgt
     6541 acggatccaa gctgcatcta ccttatgtcc gtcgcggatt ctcgtttcac atacctcctc
     6601 ctctatccga cccatcctgc gaagcgttga gcatccatga ggtcgtggcc gcgccgagag
     6661 agccactgaa ctcttcatgc gatcgttgct caacgtgcgt tcgttcttcg gattccgttc
     6721 tgcggtccac gacctcgcgc acccttcgtc tgaagaagtc gtacaggtcc tctgctatgg
     6781 cattggcctg gtctcgctcg gcctcagccg cttcaacgcg cgcctcgagc ccggccgcgt
     6841 ggatgctgag cctctcgcac tctgcggatt gcgctgagag ccggagcagt agcccgcggc
     6901 agagggcgcg ccactcctcc tgagtcggct cttcgccgcg atcccgcgac gccacggatg
     6961 ccggtaggct gatcactctt cgcccatcg
//
LOCUS       DATAIR010000666         8063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_252008, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000666 DATAIR010000000
VERSION     DATAIR010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252008"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..901)
                     /locus_tag="VHM69_15740"
     CDS             complement(113..901)
                     /locus_tag="VHM69_15740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2741895.1"
                     /translation="MLDLRGKVVIITGAGGIGSETARAFAAAGCRVAVCDRLEDRLES
                     AREKVEGTPGEVFTMVADVSNELQVRDFVEATVERWGRVDVLVNNAGYGLHKPFLKTS
                     VDEVQDQVQTNYMGVVHATKAVLEHMVPARQGHIVNIVSVVAKLPVPNSGNYAATKAA
                     LDSLSLTLRAELSEFDIAVTTVYPTAVRNTFFFKTSGSTVEETWARHFMKEPSEVAAA
                     VVGAVKNFRAEVHVGAQTRILPVVRALLPPVVRLALRWIARVYH"
     gene            complement(912..1655)
                     /locus_tag="VHM69_15745"
     CDS             complement(912..1655)
                     /locus_tag="VHM69_15745"
                     /inference="COORDINATES: protein motif:HMM:NF028265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741896.1"
                     /translation="MSWAIFLGAFVGSLIELVEVLAVVLVVGRVAGWRNALVGAGSAV
                     GLVVIAALVVGRGLALVPARPLELLAGVILLAFGQAWARSATRYYGGTLESHDDEERI
                     QEQLARGGERGGWDAVAVATAFKSSLLESFEIAIVVIGLGTAGGNWFESIGGALVATA
                     ALVCFAFWLRASLEKVPVKPAKFIAATLLMGFGTYWVGEGLGFEWPGGMLSLLWLPLL
                     WGLLMAGAAMFLRSRRGDATVTHGPPKEL"
     gene            complement(1652..1789)
                     /locus_tag="VHM69_15750"
     CDS             complement(1652..1789)
                     /locus_tag="VHM69_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741897.1"
                     /translation="MNRGDRRMLVVGGLDLGTFVTTGEVADFAASWWVADTFVSGGDL
                     P"
     gene            complement(1887..2630)
                     /locus_tag="VHM69_15755"
     CDS             complement(1887..2630)
                     /locus_tag="VHM69_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741898.1"
                     /translation="MRSDASPRSSETVVAENVGRRVLIWAGLLLLGAGAGWLLKSIAG
                     WVGSLPWAPLQGPFELAERLISSFGEPQATVGALAVGGVAGLVLAFIAEQESLTVAVA
                     HDGVITKRGGSSARIERTSVDAVFLDGNQLVLLGRSGEELAREPSDLDADRLRDAFQV
                     HGFRWLAGGDPYKVEYRLWVEDLPDLPAGADALLKARARALGKGDGGKSDAAALRAEL
                     ARLGIVVREEEKRQYWRLTREHPAGNSPH"
     gene            complement(2674..3720)
                     /locus_tag="VHM69_15760"
     CDS             complement(2674..3720)
                     /locus_tag="VHM69_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741899.1"
                     /translation="MEPTTGRDAGAKVVVDPPGTSHVDATGRVTMSRRVAPAVGLFLL
                     APLVAEYLLGNFPIGKIPALVVLAPMYGGGALLIREVARRTGRGWPTIVLLALAYGVL
                     EPGLLDNSMFNPAFQGHDFRDATWIPVLGISAGNTLAFTVGHAIWSISIPIALVETFV
                     PYRRTTPWLGKIGLSATGVLFLFGCVAVFRWAQKAEQFLPSVPQMIATAAVVVALIVA
                     AFAVGRIPLPGVDRRAPNPWFVGVLAFVASSLFFARSESWPGVAFGVSLIVAMTGLIV
                     RWSSCEGWGVAHRLALAGGALMTYAWGGFVLSALILGRTDAVDLIGNALFALGALALL
                     YAATRTVHKTKGSK"
     gene            3913..5223
                     /locus_tag="VHM69_15765"
     CDS             3913..5223
                     /locus_tag="VHM69_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2741900.1"
                     /translation="MDTDDANPTSSKPLQKERSLFASATLLKNPDRPAIFDLLFWSIL
                     AMIYAFRVFAPQIQVRGIGLAPMTAVCAALVAVWLVLPWSPYATSFRKCAAPAFLLAT
                     TAMLFASDLIWALGLYPIAFANAVFLFGLWRGVAYSAAALFALFVPAFIRSVSGTAGS
                     SPETVIQRWAVIILLSVVCMGLSATVIEARRSRERAKDLYADLESAYAELGHYAERVR
                     ELTLSEERARMSREIHDSVGHHLTAVKLQAEAAVKMAEKRPQEARAQMERARDLAAQA
                     YEEVRRSVRALGPPPLGERSGAGAMRALVRSFEGTGFEISFRLEGDERVLPEEADLVL
                     YRALQEGLTNAVRHSNARRINASLSYGGEAVKLAVTDDGQGASGKAGALREGFGLHAL
                     EERVGALGGVFAAGNTQVGGFAIEVELPVEEPFGGTCRPTGETP"
     gene            5220..5909
                     /locus_tag="VHM69_15770"
     CDS             5220..5909
                     /locus_tag="VHM69_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2741901.1"
                     /translation="MSNQPARRQVRVLIVEDQDIVRRGLATLLELEERVEVVGEAADG
                     VEALRKVPQLRPEVALVDVRMPRMDGVELTKRLAADHPRVAVIILTTFDDDEYIFGGL
                     KAGAKGYLLKNVSPEDLVWTIEKVSRGESVLGSPVASRVISELKRLGEVAARQRDSEG
                     ILSEREVEVAGLVGKGANNAEVARALYISEGTARNQVSKILKKLGLRDRTALAVHAVE
                     RGWAERSRGSS"
     gene            6124..6882
                     /locus_tag="VHM69_15775"
     CDS             6124..6882
                     /locus_tag="VHM69_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2741902.1"
                     /translation="MKVFGIDIGGSGIKGAPVDTASGKLIKERVKIKTPHPATPEAIV
                     ETAVEVVRQSGWSGPVGCGFPAVVKEGVVQTAANIDKAAIGFDMREALEGKLDAPVSV
                     VNDADAAGLAEMRWGAGRDQNGVVLMLTLGTGIGTSLFVGGRLVPNTELGHIEIRGKD
                     GEHRAAESARKREDLSWKEYSKRLDEYIHRIEDLLWPDLIVIGGGISKKSEKYFPHLT
                     TRTPLVPAEMHNDAGIAGAALAGIPERAAVSTAE"
     gene            6937..7344
                     /locus_tag="VHM69_15780"
     CDS             6937..7344
                     /locus_tag="VHM69_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2741903.1"
                     /translation="MPEVIERLRQALNRHDPEAMLECFDPDYRSEQPAHPNRGFGGRE
                     QVRKNWSAMFESFPDFEAELLRHSSDGNAVWSEWHWSATGLNMAGVTVMGIKEGRISW
                     ARLYMETVEEAGQDIDETMRTITGRDSGPGGRG"
     gene            complement(7395..7976)
                     /gene="folE"
                     /locus_tag="VHM69_15785"
     CDS             complement(7395..7976)
                     /gene="folE"
                     /locus_tag="VHM69_15785"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2741904.1"
                     /translation="MPENVDGPRIERAVREILIAIGEDPEREGLVETPGRVAEAYAQL
                     FSGLHEDPARNLEVSFPAGNHDTVLLRDVPLASVCEHHLLPFVGKAHVGYVPEERVVG
                     LSEIVYLVEGFARRPQLQERLTAQIADALYDALGSRGSVVVIEAEQLCMTMRGAQKPG
                     TITVTSAVRGVFDEEPGRRAEIMALVSRGETQG"
BASE COUNT         1596 a         2575 c         2552 g         1340 t
ORIGIN      
        1 ggggcgggag aggcgaacgt ccggtaccgc gcgctgtcag gggcggcgag agatcggtcc
       61 cagatcccct gctggaaggg aagacctacg ccgcctgacg gataagcggg agctaatgat
      121 acacgcgggc tatccaccgc agcgccagac gaactacagg cggtagaaga gcccggacca
      181 cgggcagtat tcgcgtctgg gctccaacgt gcacctctgc ccggaaattc tttacggcgc
      241 ccacgaccgc agccgctact tcagacggct ccttcatgaa gtgtctggcc cacgtttctt
      301 cgaccgttga gcccgatgtc ttgaagaaga acgtgttgcg cacggcggtc gggtacacgg
      361 tcgttacggc gatatcgaat tcggaaagtt cggcacgaag ggtcagggag agtgagtcca
      421 gcgcagcctt ggtcgcggcg tagttgcccg agttcgggac ggggagcttg gcgaccacag
      481 agacgatgtt gactatgtgg ccctgacggg cgggcaccat atgttcgagc acggccttcg
      541 tggcgtgcac gacgcccata tagttggtct gtacttgatc ctgcacctcg tccacactcg
      601 ttttgaggaa cggcttgtgt aacccgtaac cggcgttgtt gaccagcacg tccacacgcc
      661 cccatcgttc gacggttgcc tccacgaagt ccctgacctg gagctcgttc gagacgtcgg
      721 ccaccatcgt gaaaacctca ccgggggttc cttcgacctt ctcacgcgcg ctttcgagac
      781 ggtcctccag ccggtcgcag acggccacgc ggcatcctgc cgcggcgaag gctctggcgg
      841 tctccgagcc gatgccgccg gcgcctgtga tgatgaccac tttgccgcga agatctaaca
      901 tctacctggc ctcatagctc cttcggtggt ccgtgggtca ccgtggcatc gccgcgcctc
      961 gaacgtagga acatcgccgc tccggccatc aggaggcccc atagtagagg cagccagagc
     1021 aaagaaagca tcccgccggg ccattcgaac cctaacccct cgccgaccca gtacgtcccg
     1081 aaacccatca acagcgtcgc cgctatgaac ttggcaggtt tgaccggcac cttctccagg
     1141 gaagccctca gccagaaagc gaagcatacg agtgccgcgg tggcgaccag cgcccctccg
     1201 atcgactcga accagttccc gcctgccgtg ccgagcccga tcacgactat ggcgatctcg
     1261 aagctctcca gaaggctgct cttgaaggcc gtcgcgacgg caacggcgtc ccagccaccc
     1321 cgctccccgc cccgcgcgag ttgctcctga atgcgctctt cgtcatcgtg cgactcgagt
     1381 gtgccaccat aatatctcgt agcggagcgt gcccacgcct gcccgaaggc gagcaatatc
     1441 acccctgcga gaagctcgag cgggcgagcc gggacaagcg cgaggccgcg ccccacaacc
     1501 aatgcggcaa tcaccaccag cccgaccgcg ctccctgcgc caaccagggc gttgcgccat
     1561 ccggcgaccc tgccgaccac gagcaccacc gccaacacct cgacgagttc gatcagggac
     1621 cccacaaaag cgccaaggaa gatggcccag ctcatggcaa atctcctccg gaaacaaacg
     1681 tatccgcgac ccaccaggag gccgcgaaat cagccacttc cccggtcgtc acgaaagtgc
     1741 cgagatccag gccaccgacc acgagcatgc gccgatcgcc cctgttcaaa tccttcaatg
     1801 gtgtatcgtt gacattgaga gccattcgca ataaccgcat aggcgacacg atacacgccc
     1861 tggaaggaga aagcaacgca tctgcatcaa tgagggctgt taccggccgg gtgctctcgg
     1921 gtcaggcgcc aatactggcg cttctcctcc tcgcggacca cgatgccgag cctggcaagt
     1981 tccgcgcgca aagccgccgc gtcgctcttg ccgccgtcgc ccttgccgag cgcccgcgcg
     2041 cgggccttca gcaaggcgtc ggcgccggcc ggaaggtcgg gtaagtcctc cacccagagg
     2101 cggtactcga ccttgtacgg atcgccgccg gccagccagc ggaacccgtg gacttggaag
     2161 gcatcccgca ggcggtcggc gtcgaggtcg gaaggttccc tggccagttc ctcacccgac
     2221 cttccgagga gaacgagttg attcccgtcg aggaaaaccg cgtccaccga agtgcgttcg
     2281 atcctagccg aggatccgcc gcgtttcgtg attacgccat cgtgggcgac ggcgacagta
     2341 aggctctcct gttccgcgat gaaggcgagt accagacctg ctacccctcc gacggcgaga
     2401 gcgccgaccg tggcctgcgg ctcgccgaag gacgagatca gccgctcggc gagctcgaac
     2461 gggccctgca gcggcgccca cgggagagat cccacccagc ccgctatgga cttcagcagc
     2521 catccagccc cggcgccaag aagcaggagc ccggcccaaa tgaggacacg cctccccaca
     2581 ttttcggcta caacggtctc gctgctgcgt ggtgacgcat ctgatctcat ttactcactc
     2641 cttgatgaac gactgcggac tgcttatacg agttcatttc gatcccttcg tcttgtgcac
     2701 cgtgcgggta gcggcataaa gaagggcgag cgcgccgagg gcgaagagcg cgttgcctat
     2761 caagtcgacc gcgtccgtgc ggccgaggat cagtgccgaa aggacgaacc cgccccaggc
     2821 ataagtcatc agcgcgccac ctgccagggc aagacggtgg gcgacacccc aaccctcgca
     2881 tgacgaccat cggactatca ggccggtcat cgcgacgatc agtgagacac cgaaagcgac
     2941 gcctggccag ctctcgctcc tggcgaagaa caggctcgaa gcgacgaacg ccaatacgcc
     3001 gacgaaccat gggttcggcg cccgccggtc gacgccgggg agaggaatcc taccgaccgc
     3061 aaaagccgcg acgatcaggg ccaccacgac ggcagccgtt gctatcatct gcggcacgga
     3121 cgggagaaac tgctccgcct tctgggccca gcggaagacg gcgacgcagc cgaagaggaa
     3181 aagtacgccc gtggccgaca ggcctatctt gcccagccac ggcgtcgtcc tccgataggg
     3241 aacgaaggtc tcgacgaggg cgatcggaat acttatgctc cagatcgcgt gcccgaccgt
     3301 gaatgccaaa gtgttgccgg cactgatccc aagcaccggt atccacgtgg cgtcacggaa
     3361 gtcgtggccc tggaacgcgg ggttgaacat cgaattgtcg agcaggcccg gctccagcac
     3421 accgtacgcc agcgctagca agacgatggt gggccagccg cgacccgtgc ggcgtgccac
     3481 ttcgcgaatc agaagcgctc cgccgccgta catcggtgcg aggacgacga gggccgggat
     3541 cttcccgatc gggaagttac ccagcaggta ctccgccacc agcggggcca gcaggaaaag
     3601 tcctactgcg ggagccactc gccgcgacat cgtgactcgc cctgtggcgt ccacatggct
     3661 ggttcccggc gggtcgacga cgactttcgc gccggcgtct cttccagtgg taggctccat
     3721 cggacctccc tttctcgccc gggtcgctct tcatattcga atctagcaac gtgcgcttcc
     3781 gtggtaatct gctgccgatc attttgtttt caccttcttc cgtgagcgcc tcatacggta
     3841 tcgttacatc cgtcacgagg aaagtgacat ccgtccgatg accggcggct cagaaggggt
     3901 tagagttgga tcgtggacac agacgacgca aacccaacgt cgagcaagcc gctccagaaa
     3961 gaacggtcgc tcttcgcgtc cgccactctc ctgaagaatc ccgaccggcc tgcgatcttc
     4021 gatcttctct tctggtccat cttggccatg atctacgcct tccgagtgtt cgcaccccag
     4081 atccaggtcc ggggcatcgg gctggcgccg atgactgcag tctgcgccgc cctcgtggcg
     4141 gtgtggcttg tgctgccctg gtccccttac gcgaccagct tcaggaagtg cgcagccccg
     4201 gccttcttgc tggccacgac cgccatgctc ttcgcgtcgg acctgatctg ggcgctaggg
     4261 ttgtacccga ttgccttcgc caatgcggtc tttctgttcg ggctatggcg cggagtcgcc
     4321 tactccgccg cagccctgtt cgctctcttc gtgcccgcct ttatccgatc cgtctccggt
     4381 accgctggct cttcaccgga gaccgtcatc caaaggtggg cggtgatcat cctcctaagc
     4441 gtggtctgca tgggcctcag cgcaactgtg atagaagcga ggcgaagcag ggagcgggcg
     4501 aaggacctgt acgcggacct tgagtcggcc tacgcggagc tcgggcatta cgcggaaagg
     4561 gtgcgtgagt tgaccctatc cgaggagcgt gcccggatgt cgcgggagat ccacgactcc
     4621 gtcggccacc acctcaccgc cgtaaagctg caggccgagg ccgcggtgaa gatggcggag
     4681 aaaagaccgc aggaggcccg agcgcagatg gaaagggcca gggatctggc cgcgcaagcc
     4741 tacgaggagg tgcgccgctc ggtgagagca ctgggtccac cacctctggg agagaggtcc
     4801 ggcgctggcg ctatgcgggc gctagtcagg agcttcgagg ggaccggatt cgagatcagc
     4861 ttccggctcg aaggagatga aagggtcttg ccggaggagg cggatctggt cctctaccgt
     4921 gcgctgcagg aggggctgac gaacgccgtc agacattcga acgcccgccg gatcaacgcc
     4981 tcactttctt acgggggaga agccgtgaag ctggcggtga ccgacgatgg gcagggcgcg
     5041 tcgggcaaag ccggagcgct gcgggaagga ttcggacttc acgccctcga agagcgggtc
     5101 ggggcgctgg ggggcgtctt cgccgccggg aacacacaag tcggaggatt cgccatcgag
     5161 gtcgaattgc ctgtcgaaga gcctttcggc ggtacgtgcc gcccgacggg ggagacaccg
     5221 tgagcaatca gccagcgcga agacaggttc gggtgctgat cgtcgaggac caggacatcg
     5281 tgcgccgggg cctggctacg ctccttgagt tggaggagcg ggtggaggtc gttggagagg
     5341 ccgccgacgg cgtcgaggct ctccgtaaag taccgcagtt gaggcccgag gtcgcgctgg
     5401 tggacgtgcg catgcctcgg atggacggcg tcgagcttac caagcggtta gccgccgacc
     5461 atcccagagt cgcggtgatc atcttgacca ccttcgacga cgacgagtac atcttcgggg
     5521 gtttgaaggc cggagcgaag ggctacctgc tcaagaacgt ctcgccggaa gatctcgtct
     5581 ggaccataga gaaagtgagc cggggcgagt cggtgctagg cagtccggtc gcatcgaggg
     5641 tgatctccga gctcaagcgt ctgggagaag tcgcggcccg acagcgcgac tctgaaggca
     5701 tcctctccga gagggaggtc gaagtggcag ggctcgtggg taagggcgcc aacaacgcgg
     5761 aggtggcccg cgcgctgtat ataagcgagg gcacagcgag gaaccaggtc tccaagatcc
     5821 tcaagaagct aggtctgcgc gaccgaaccg cgctggccgt ccacgccgta gagcgcggat
     5881 gggccgaacg ttctcgcggg agttcctagc cccgggtcag acgcagctca accgtgcttc
     5941 gcagagggcc acagcacaat cctcccggaa tcatgccgta ccccgatcca agctggtccg
     6001 cgtcttggca ggagcagccc gcccttacac ttgtccaata gcgtcaccga cctttctcag
     6061 ggaagcgggc ggcggcgtgg gatcggctat aaaatgacgc aagtctcagg ctggggagga
     6121 tgcatgaaag tattcggcat agacattgga gggtccggga tcaagggcgc gcccgtggat
     6181 acggcgtccg gcaaactcat aaaggagagg gtaaagatca agacgcccca tcctgcgaca
     6241 cccgaggcaa tcgtcgagac ggcggtcgag gtagtacgcc agtccgggtg gagtggacct
     6301 gtcggatgcg gcttccctgc cgtcgtaaag gaaggtgtcg tgcagacggc ggcgaacata
     6361 gacaaagcag ccataggctt cgacatgcgc gaagcgttgg agggaaaact cgacgctccc
     6421 gtgagcgtgg tcaacgacgc tgacgcggcg ggcctcgccg agatgcgctg gggcgctggc
     6481 agggaccaga acggcgtcgt cctcatgctc actctaggga ccggcatcgg cacctccctc
     6541 ttcgtcggag gcaggctcgt gccgaacaca gagctcgggc acatcgagat caggggcaag
     6601 gacggcgagc acagggctgc ggagagcgcc cgcaagcgcg aagacctgag ttggaaggag
     6661 tactcgaagc gtctcgacga gtacatccac aggatcgagg acctcctctg gcccgatctc
     6721 atcgtcatcg gcggcggtat cagcaagaag tccgagaagt acttccccca tctcacgacg
     6781 cgcacaccac ttgtgcccgc cgagatgcac aacgacgccg gtatcgcggg ggccgcactg
     6841 gccggtatcc cggagagagc agccgtctcg acggcagaat aatgccggcc gcgaggatgt
     6901 ggctttcgta attcaactcg agaaggagcc ggcgtcatgc ctgaagtgat cgagcgtctt
     6961 cgtcaagccc tgaacagaca tgacccggag gcgatgctcg agtgcttcga tcccgactac
     7021 cgcagcgagc aaccagccca tccaaaccgg ggcttcggag gcagagaaca ggtgcgcaag
     7081 aactggtcag ccatgttcga gagcttcccg gacttcgagg ccgagttgct aaggcacagc
     7141 tccgatggaa acgcagtgtg gagcgaatgg cattggagcg caacggggtt gaacatggcc
     7201 ggggtaaccg tcatggggat caaggagggc aggatctcgt gggcgcgcct ttacatggag
     7261 acggtcgagg aggccggcca ggacatcgac gaaaccatgc gaaccattac aggaagagac
     7321 tcaggccccg gaggacgcgg ctaatcgtta gccccggtcg ccggcggact cagggagacc
     7381 ttagctccgc ggccctaacc ctgtgtctct ccacgtgaga ccagagccat aatctctgcc
     7441 ctccgccccg gttcctcgtc gaagacgccc cgtacggccg aggtaacggt gatggtgcct
     7501 ggcttctgcg cccctctcat ggtcatgcac agttgctccg cctcgatgac aacgacggaa
     7561 ccacgcgatc ccagagcgtc ataaagagcg tcggcgatct gggccgtcag gcgttcctgg
     7621 agttgcggcc tgcgggcgaa accctcgacc aggtacacga tctccgatag ccccactacg
     7681 cgctcctctg gcacgtaccc gacgtgggct ttgccgacga aggggagcag gtgatgttcg
     7741 caaacactcg ccagcggcac gtcccgcagc aggacagtgt cgtggttccc agccgggaag
     7801 gagacctcca ggttacgcgc tgggtcttcg tggaggccag agaaaagctg ggcgtaagct
     7861 tctgcgacac gcccgggtgt ttcgaccaga ccctcccgct ccgggtcctc gcctatggcg
     7921 attaggatct cacgcacggc ccgctcgata cgcggcccat ctacgttctc cggcatcgga
     7981 tcacccctcg acgatcccgg gtagactatt caggtcggag acgacgaagg tagcctcggg
     8041 cgcctcgatg ttgcctttgc ggt
//
LOCUS       DATAIR010000667         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_160057, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000667 DATAIR010000000
VERSION     DATAIR010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160057"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VHM69_15790"
     CDS             complement(<1..854)
                     /locus_tag="VHM69_15790"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741905.1"
                     /translation="MSSRRNNLIVLALVVVLLGVAAYFIFINEPVTESTQLGLDLQGG
                     VSAQLEGSQTGGGKVTHQEMVQAADLIRQRIDRLGVAEPDVRVQSEDQIVVQIPGVKN
                     PDEVIQIIGSTAQLGFYQVLAFDGLPNVPQDEIDQAEEDIRESLKDDDTYEKGKTKIL
                     FEESPALKGPGTAVSGYVVHKNPDLTGDALQQNGANVEFDQTTGKRIVSLQLTAEGGR
                     QMTELTQKMLSDAAVSGEPAQLAIALDQDIQSAPTVQEPLARNISISNDSLPEGLPED
                     EAKQLETVL"
     gene            complement(877..>1899)
                     /gene="tgt"
                     /locus_tag="VHM69_15795"
     CDS             complement(877..>1899)
                     /gene="tgt"
                     /locus_tag="VHM69_15795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX2741906.1"
                     /translation="TKGTVKGLTPRDLRETGAGIVLGNTYHLYLRPGSDLIAEAGGLH
                     AFMGWEGPILTDSGGYQVFSLAQTRRISEEGVEFASVYDGSKHVFTPELTTRVQEELG
                     SDVAMVLDECPPANASSEYHQDSLRRTARWARRCKEAHGRRDQALFGIVQGGLYPDLR
                     EESLGRTVEIGFDGYAVGGLSVGESREDMLEALALTASMLPSEKPRYFMGIGDPVGIL
                     QVIALGVDMFDCVLPTRLARHGAALTPDGRLNLKNAKYRKDFGPLDPECSCEACTGYS
                     RAYLAHLVRENELLGHRLVTLHNVSFTVDLCRVARREIQAGRYEAWSRAWISRYEASG
                     LPFKGR"
BASE COUNT          313 a          632 c          558 g          396 t
ORIGIN      
        1 agcacggtct ctagctgctt cgcttcgtct tcagggaggc cttcaggaag actgtcgttg
       61 gagatggaga tgttccgcgc cagcggttct tgcacggtag gcgcgctctg gatgtcctgg
      121 tcgagcgcga tggcgagttg cgcgggctcg ccgctcacgg cggcgtcgct caacatcttc
      181 tgggtgagtt ccgtcatctg ccgcccacct tcggcggtca gttggaggct tacgatgcgc
      241 ttccccgtgg tctggtcgaa ctcgacgttc gccccgttct gctgcagcgc atcgcccgtc
      301 aggtcgggat ttttgtgaac cacgtatccg ctgacagccg tacccggtcc cttaagagcc
      361 gggctctcct cgaacaggat cttcgtcttc cccttctcat aggtatcgtc gtccttgagg
      421 ctctccctga tgtcttcttc tgcctggtcg atctcgtcct gcgggacgtt cggcagccca
      481 tcgaaggcga gcacctggta aaagcctagc tgggccgtgc tgccgatgat ctggatgacc
      541 tcgtcgggat tcttcacgcc ggggatctgg accacgatct ggtcctccga ctgtaccctg
      601 acgtcgggct ctgcaacgcc gagacggtct atacgctgcc ggatcagatc agccgcctgc
      661 accatttcct ggtgggtgac cttgcccccg ccggtctgag aaccttctag ctgcgcgctg
      721 acaccgccct ggaggtcgag gccgagctgc gtcgactccg taacgggctc gttgatgaag
      781 atgaagtagg ctgccactcc gaggaggacg accaccagcg ccagcacgat caggttgttt
      841 cgacgactac tcatataatc ggagcgttat tctagctcat ctgcccttga atggtagccc
      901 tgaggcttcg taacgtgaga tccaggcccg cgaccacgcc tcgtagcgtc ccgcctgtat
      961 ctcccgccgc gccacccggc acagatcgac ggtgaagctt acgttgtgca gcgtgacgag
     1021 cctgtgaccg agaagctcgt tctctctaac caggtgcgcg aggtaggccc ggctatagcc
     1081 cgtacacgcc tcgcaggagc actctgggtc gagcggcccg aaatctttgc ggtacttcgc
     1141 gttcttgagg ttgagtcggc catccggggt gagggccgcg ccgtggcggg ccaggcgcgt
     1201 cgggagcacg cagtcgaaca tatcaacccc gagggctatg acctgcagga tgccgacggg
     1261 gtcccctatc cccatgaagt accgcggttt ctcggagggc agcatagatg ccgtgagagc
     1321 cagcgcttcc agcatatctt cccgcgattc ccctaccgag agacccccga cggcgtagcc
     1381 gtcgaacccg atctctaccg tccgacccag gctctcctcc ctcagatctg gatacagtcc
     1441 gccctgcacg atcccgaaga gcgcctgatc ccttctcccg tgcgcctcct tgcaccgccg
     1501 cgcccacctc gcagtccgcc gcagcgaatc ctgatgatac tcactgctcg cgttggcagg
     1561 cgggcactcg tcgaggacca ttgccacgtc cgacccgagt tcctcctgta ctctcgttgt
     1621 caactccggg gtaaagacat gcttcgaccc gtcgtagacg gaggcgaact cgaccccctc
     1681 ctccgagatg cggcgcgtct gggcgagcga gaagacctgg tagccgcccg agtcggtgag
     1741 gattggacct tcccaaccca tgaaagcgtg gagcccaccc gcctccgcta tgaggtccga
     1801 gccgggtctg agatagagat gatacgtgtt cccgagcacg atgccggccc cggtctccct
     1861 caagtcccgt ggcgtcagtc ctttgaccgt acccttggt
//
LOCUS       DATAIR010000668         8924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_205995, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000668 DATAIR010000000
VERSION     DATAIR010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205995"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VHM69_15800"
     CDS             complement(<1..777)
                     /locus_tag="VHM69_15800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2741907.1"
                     /translation="MHAGHSSRILFTAHQFFPESRAGVEVVTLGLAQELKARGHEPFV
                     LAAKRTIPYSELRPGETEDYEFEGIPVRRVGRPEESLFRPYRLNYRNDDMAMRVREYV
                     REIAPDIVHAMHLQGLSAGVLPVFKEFGLPVVFTAADFWTVCPVVDLRRHDGVMCKGP
                     EVAHCVRCIASRSADPRIKRAASLLPGVVPTAADVISRTPLSRFSLPLRQVEDVRERP
                     SYIRERMESVDRVLAYTRLARDLLLANGIEEEKVHVSHYGI"
     gene            complement(800..1759)
                     /locus_tag="VHM69_15805"
     CDS             complement(800..1759)
                     /locus_tag="VHM69_15805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2741908.1"
                     /translation="MSTEPGSPKASIVVTTLNAGPGFGDLLRRLFSQKADFDYEVLVI
                     DSGSTDATIELALRCGASVHRIHRDEFDHGATRNLGASLSRGRYVAFVVQDALPLDDR
                     WLASMVENFERDETVAGVYGRQIPRPESSPLTRALMNDWPVAGVERREQFAGGAYREM
                     SPHSRRSLATFDNVSSCVRRSVWEDLPFERTGFGEDLRWGKKAVEAGYKLVYEPRSTV
                     LHSHERGALYDLRRYYADGLVLQELFGLAPTPNLARLLLNVLLSSAHLYLRLIQNDKR
                     VAPRSLLLAMRYALFSQAGAYLAARRSRAGTSARLHSFLLKGI"
     gene            2118..2996
                     /locus_tag="VHM69_15810"
     CDS             2118..2996
                     /locus_tag="VHM69_15810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741909.1"
                     /translation="MASRHDTNVYFNSNSSHAQIVELVGYDRRVLDVGTSTGYLAEAL
                     VERGCSVTGIELDPEAARQAGKYCDRVIVGDVESLDLGAEPGEESFDVIVFGDVLQHL
                     KNPLRALERLKPFLDAEGCVVASIPNIAHGSVRLALMQGEFRYRPQGLLDNTHLKFFT
                     RKTVEKLFEDAGLLITESRRTTLGVFDTEIEIDRERVTEDVLRQVEEDPESLTYQFVL
                     RASPVGEAATLAHYEKLLYALARKLRGSEHTQRLLDIRTKQLAEKEQEVASLAQQVID
                     LNNRLAKMVQFGREEA"
     gene            2993..4093
                     /locus_tag="VHM69_15815"
     CDS             2993..4093
                     /locus_tag="VHM69_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741910.1"
                     /translation="MRTEDFASPQENAVWADLTQPLDRLVPASSEAAYDRLVGYGFAR
                     RYVEGKTVADIGWEDIGYGSHLLAEKSAFVSAMSVSVEAVDIALKYYSAPNASYEKIY
                     LTDLPYPEEHFDVVVALRMIENLDQPEDLVREASRVLKQDGIFVVSVPDKQGNAEDRP
                     RMGMHASQFQELLERHFEQVRTYRQGAVAGGSIIPASGDISGALVEGASLSPSAPSFG
                     PERPTSRHIVAVCGGAEALENAEQPYLVIDRDRRVFDECADQAKDLDLLATEIKNMEK
                     TEVQTFRDALWLRGSEIAYLRAQIRDQIRNSEARTRRSEAQIRSNEAQIQYLKQQIHE
                     MENSTTWRMFEPYRRLRAWLGTTKGSDDHRPD"
     gene            4203..6941
                     /locus_tag="VHM69_15820"
     CDS             4203..6941
                     /locus_tag="VHM69_15820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2741911.1"
                     /translation="MRLREEASTFRYRPLISILMPVIEREPEQLEQALDSVLGQIYQH
                     WELCVGCNDTTGGRTKRILSEYERLDGRIKVVYLGRSAAGLSNAVLSIAAGEFAGVLG
                     CCDELAPDALFEVVKALQEHADSDLIYSDEDRIDEKGNRSRPHFKPDWSPDLLLSTNY
                     VSHLSVYRRSVLEDVGAFRKGFEGCLGYDLVLRVTERDGLIQHVPKVLYHRRISEAPK
                     ASGEGDEVTTRESARRALSEAMKRRGLEGSVEDGLLPDRFRLRFDVRGEPKVSIIIPT
                     RDNVSLLKNCVESIERLSTYRNYEILIVDNDSVDSETVEYLASMVHRVVRFRDEFNYS
                     RINNLAVAQAEGEYVLLLNDDTEVISADWLEVMLGHAQRPEVGAVGARLLYPDRRIQH
                     AGVLVGTGNPWGPGIALHSHQYYPADSAGYAGTALTTTNYSAVTAACLLLRKSLFEEV
                     GGLDEENLPVGFNDVDLCLRIRERGFLITYTPHAELYHHESASRGHGGGDPAEALYMR
                     DRWGTVMDEDPYYNPNFSKGSGDFNLRADLLRPRVLRPEVEQESAVSFMNSLTTPREE
                     LKEYVMAQQGAARNSSRNVIVPASEDRNGSSPLLKTFEPLEDPGPQEDERPIRSFAAP
                     VDREALRTEQLIWIFGSPRTGSTWLSCIMAELDNQKRWHEPYVGLLFGSFIYERLGES
                     SKLLNTTGFIMGEAHRKVWLNSIKNFVVDGAMARYPRLRRNQYLVVKEPNGSVGAPLL
                     LEATPESRLIFLIRDPRDVVASRLDAFRAGSWSAQQRVYSTVAELNAFTKHLAEEYVN
                     VVSQVKRAYEAHAGRKTLIRYEDLRYDTSSTLAAMYEALGVEFDRARLEAAVAKHSWE
                     QIPPADKGTGKFYRKAQPGGWKEDLTQEQVSLVVDVTRPMIREFYPPDENGYTFSG"
     gene            complement(6923..7942)
                     /locus_tag="VHM69_15825"
     CDS             complement(6923..7942)
                     /locus_tag="VHM69_15825"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2741912.1"
                     /translation="MFEGLRARVLGSGRAMRRAAKKNRTLIRVYGRLIGERERAYLEL
                     VRLRSRLGAKSVRAGIDPGRMVWIFGSGRSGSTWLRSMMGEIGGFRVWEEPMVGRLFG
                     EFYARAPKENLRSADFVMGDPIRKGWIESVRNFVLDAARYSNPRLGAEEYLVVKEPNG
                     SVGAPLLMEALPESRMILLVRDPRDVVASVLDAAREGGWLYASREGKTGTRRGRVDRR
                     PNAFVRERAKVYRRGVESARLAYDAHGGPKVLVRYEDLRSDTMNEMRRIYSTLGVPVD
                     ENELRRAVEKHSWENVPEGEKGRGKFYRKGTPGGWKEDLSPEQVQIVEKVTGQLLKEL
                     YPENV"
     gene            8113..>8924
                     /locus_tag="VHM69_15830"
     CDS             8113..>8924
                     /locus_tag="VHM69_15830"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2741913.1"
                     /translation="MRHKWLVFLPVLAVITMAASGAGVEARQASLSDEFGRASEVYGV
                     PKELLLAMGYVNTRWEMPPPDASDYEAGDPEGRGNYGVMALVKNPSTDTLGEAASLTG
                     LSERDLKTDRASNVLGGAAVLAELAGSQRPSDLGGWYDAVGRYGNGALYAQQVYEVLK
                     SGASADISTGEHLELAPQSVDVPPIYTTQATGDYSESTWYGNGGGNYTNSNREATYDI
                     NKIVIHVTQGSWSSAINWFANSDNKQSSAHYTVRSSDGFIGQSVREEDIAWHA"
BASE COUNT         1813 a         2756 c         2759 g         1596 t
ORIGIN      
        1 gataccgtag tgggagacat gtaccttctc ctcttctatg ccgttggcaa ggagaaggtc
       61 tcgtgcgagc cgggtgtagg cgagaacacg gtccacggat tccatccgct cccttatgta
      121 cgatggacgc tccctcacgt cctcaacctg gcgcagtggg agggagaagc gcgagagcgg
      181 tgtgcgagag atcacgtcag cggccgtcgg gaccacacca ggtagaaggc tcgcggccct
      241 ctttatgcgg gggtccgcgc tcctgctggc tatgcaccgg acgcagtgtg ccacctcggg
      301 tcctttgcac atgacgccat cgtgcctcct aaggtcgaca acggggcaga ccgtccagaa
      361 atctgccgca gtgaagacta cgggcaagcc gaactcctta aagacaggta gcacgcccgc
      421 cgagagcccc tgcaggtgca tggcatggac gatatcagga gctatctccc gcacgtactc
      481 ccgtaccctc atagccatgt cgtcgttcct gtagttcagc cggtaggggc ggaacaggct
      541 ctcctcagga cgaccaaccc tcctgactgg gatgccctcg aactcgtaat cctccgtctc
      601 acctggcctg agttcgctgt agggaatagt gcgcttggcg gccaggacaa agggctcgtg
      661 tccgcgtgcc tttagctctt gggcgagccc gagcgtcacg acctccacgc cagcacggct
      721 ctcggggaag aactggtggg cggtaaagag gatccttgaa gagtgtcccg cgtgcacaat
      781 gaaggattat acggaatcgc tagatacctt ttagaaggaa ggagtgcagc ctggcggaag
      841 tcccggccct tgatctgcgg gccgccaggt aggcgcctgc ctgggagaag agagcgtacc
      901 tcatggcgag tagcagggac cggggagcta ctctcttgtc gttctgaatg aggcggaggt
      961 agaggtgcgc ggaggagagg agcacgttca ggagcagcct tgccaggttc ggcgtcggag
     1021 caagcccgaa gagctcctgg agaaccagcc cgtccgcgta gtagcggcgc aagtcgtaga
     1081 gagcgccgcg ctcgtgggag tggagtacgg tcgagcgcgg ctcgtacacg agtttgtagc
     1141 cagcctcgac ggccttcttg ccccacctga ggtcttctcc gaagcccgtg cgctcgaagg
     1201 gaagatcttc ccacacggag cgccgcacgc aggagctcac gttgtcgaag gtggcgaggg
     1261 agcgccgcga atgcggggac atctcgcggt aggctccgcc ggcgaattgc tcccggcgct
     1321 cgacacctgc gacgggccag tcgttcatca gggcgcgggt cagcgggcta ctttcggggc
     1381 gtgggatctg tcgcccgtac accccggcca ccgtctcgtc gcgctcgaag ttctcgacca
     1441 tcgaagcgag ccacctgtcg tccagtggca gtgcgtcctg cacgacaaag gccacgtacc
     1501 ttcccctgga gagcgaggca cccaggttgc gcgtcgcgcc atggtcgaac tcgtcccggt
     1561 gtatccggtg gacggatgcg ccacagcgca gggccagctc gatggtagcg tctgtcgagc
     1621 cggagtcgat cacgagcacc tcgtagtcga agtccgcctt ctgggagaag agcctgcgga
     1681 gcaggtcccc gaaaccggga cccgcgttca gagtcgtgac gacgatcgac gccttcggtg
     1741 agcccggctc agtcgacaaa cgcggtcggc accctcaacg cttcgcgggg atcactaccg
     1801 tctgtcagta tggcctttcc tgctctgttc aagacaagcc tcacgagcgc tatgctagca
     1861 tgccaggtgg actgtgcggc cgccccgttc agcagggcgg ttatgcgctc gaccgtcccg
     1921 gcctctcccg gagtgaggtc gttcctccat gcggccgata agctaggatg acaaaatggc
     1981 tatcatgtcg atgtcctggg agagaacggg cactatcgac atggacagag gctccagata
     2041 agatgcgtag ggtatcctgg aaagctagaa cgcggatgga tgccgccact acaatcgaga
     2101 gtttcgagcg gagaaggatg gcgagcaggc acgacaccaa cgtatatttc aacagcaact
     2161 cctcccacgc ccagatcgtc gagctcgtcg ggtacgacag gcgcgtgctc gacgtcggaa
     2221 cctcgaccgg ttacctggct gaggccctcg tagagcgcgg gtgcagcgta acggggatcg
     2281 agcttgatcc cgaggccgcc agacaggcgg gaaagtactg cgacagggtc atagtcgggg
     2341 acgtggagag cctggatctc ggcgcggaac cgggcgagga gtcctttgac gtgatcgtct
     2401 tcggggacgt gctgcagcac ctgaagaacc cgcttcgagc actcgaacgt ctcaagcctt
     2461 tcctcgatgc ggagggctgt gtggtcgcct cgatcccgaa catagcccac ggcagcgtac
     2521 gcctggctct catgcaggga gagtttcgct accgcccgca gggtctgctg gataacacgc
     2581 atctgaagtt ctttacacgc aagacagtgg agaaactgtt tgaagacgcg gggctcctga
     2641 tcaccgagtc gaggcgtacc acgctcggcg tcttcgacac cgagatagag atagacagag
     2701 aacgggtaac ggaggatgtt ctacggcagg tagaagaaga cccggaatct ctcacctacc
     2761 agttcgtgct cagggccagt cctgtcggtg aagcggcaac gctggcccat tacgagaagt
     2821 tgctctacgc gttggcaagg aaacttcgcg gctccgagca tacccaacgc ctgctggaca
     2881 tcagaaccaa acagcttgcc gaaaaggagc aagaggtggc gagcctcgcg caacaagtca
     2941 tcgatctcaa caatcggttg gcgaagatgg tgcagttcgg cagagaggaa gcatgaggac
     3001 ggaggatttc gcctcgcctc aagagaatgc cgtgtgggct gatctgaccc agcccctgga
     3061 tcgactcgtg cccgcgtcgt ctgaggccgc ctatgatcgg ctggtcggtt atgggttcgc
     3121 ccgtcggtat gtggaaggaa agactgtagc ggatatcggc tgggaagaca tcggatacgg
     3181 ctcccacctg ctcgccgaaa agtccgcctt cgtctcggcc atgtccgtct ccgtcgaagc
     3241 agtagatata gcgctgaaat actactcggc accgaacgcg agctacgaga agatatacct
     3301 caccgacctg ccatatcctg aggaacactt cgacgtcgta gtagcactcc ggatgatcga
     3361 gaacctcgac caacccgaag atctggtgcg ggaggccagc cgtgtcctca agcaggacgg
     3421 gatatttgtc gtctccgtcc ccgacaaaca gggcaacgcc gaagatcgtc cccggatggg
     3481 aatgcacgcc tcccaattcc aggaactgtt ggaaaggcac ttcgagcagg tacgcaccta
     3541 tcggcaaggt gccgttgccg gcggctccat cattcctgcc tccggggaca taagcggcgc
     3601 attagtggag ggcgcctccc tctctccgtc agcccctagc ttcggcccgg aacgtccgac
     3661 ctcccggcac atcgtggccg tatgcggcgg tgcggaggct ctcgagaacg cggagcagcc
     3721 gtacctggtc atcgatcgcg accggcgcgt gttcgatgag tgcgcggatc aggccaagga
     3781 cctggacctc ctggccaccg agataaagaa catggagaag accgaggtcc agacattcag
     3841 ggacgctctc tggcttcggg ggagcgagat agcctacttg agagcacaga tcagggacca
     3901 gatacggaac tctgaagccc gaacgcggag atctgaagcc caaatacgga gcaatgaggc
     3961 ccagatacag tacctgaagc agcaaatcca cgaaatggag aattcgacga cctggcggat
     4021 gttcgaacct tacaggcggt tgcgtgcatg gctgggcacc acgaaaggaa gcgacgatca
     4081 ccgaccggat taggagtttc tcgccgttcg gcggtccttc cactcgggaa gtccgtcgct
     4141 cttacgtaga ggagcagtac cctgcccttc tcagaaataa cggtctcagg gcgggggacc
     4201 tggtgcgcct gcgcgaggag gcttcgacct tccggtacag gccgctgata agcatcctga
     4261 tgccggtcat cgagcgggaa cccgagcagt tggagcaggc gctggattcg gtactgggtc
     4321 agatctacca gcactgggaa ctatgcgtcg ggtgcaacga cactaccggt gggcggacga
     4381 agagaatcct ctccgagtac gaacgtctgg atgggcggat caaggtcgtg tatctggggc
     4441 gaagcgccgc cgggctctcg aacgcggtcc tctccatcgc cgccggagag ttcgcgggcg
     4501 ttctgggctg ctgcgacgag ctcgcgccgg acgccctctt cgaagtcgta aaggccctgc
     4561 aggagcacgc cgactcggac ctcatctact ccgacgaaga caggatagat gaaaagggga
     4621 acaggtcgcg acctcacttc aaacccgact ggtctcctga cctgctcctg tccaccaact
     4681 acgtgtccca cctcagcgtc tacaggagga gcgttctgga ggatgtcggc gccttcagga
     4741 aaggcttcga gggttgcctc ggctacgatc tcgtcctgcg tgtcaccgag cgggacgggt
     4801 tgatccagca cgtaccgaag gtcctgtacc acagacgaat ctccgaggcc ccgaaggcat
     4861 cgggcgaagg agatgaggtc actacaagag agagcgcacg tcgcgccctc tccgaagcca
     4921 tgaagcgccg cggcctcgaa ggttccgtcg aagacggcct cctccccgac cggttccggt
     4981 tgcggtttga cgtgcggggc gagccgaagg tgagcatcat catcccgact cgggataacg
     5041 tctctttgct caagaactgt gtggagagca tcgagcgcct gagcacctac cgcaactacg
     5101 agatactgat agtggacaac gacagcgtcg attctgagac cgtcgagtac ctggcctcga
     5161 tggttcatcg ggtggtcagg ttccgagacg agttcaacta ctccaggatc aacaacctcg
     5221 ccgtcgccca ggccgagggg gagtacgttc tcctcctcaa cgacgacacc gaggtgatct
     5281 ccgcagattg gctggaagtg atgctcgggc acgcccagag gccggaggtg ggggccgtcg
     5341 gggccaggct cctctacccc gaccgccgca tccagcacgc cggggtactg gttggaaccg
     5401 gcaacccctg gggacctggc atcgccctgc attctcatca gtactatccg gctgactccg
     5461 cgggctacgc gggcaccgcg ctgacaacca ccaactacag cgccgtgacg gcggcctgcc
     5521 tgttgctacg caaatccctg ttcgaggaag tcgggggcct tgacgaagag aacctcccgg
     5581 taggattcaa cgacgtggat ctgtgcctgc ggatacgcga gcgggggttc ctgatcacct
     5641 acactcccca cgccgagctt tatcatcacg agtccgcgag ccggggacac ggtggcgggg
     5701 atcctgcgga agccctgtac atgagagacc gctggggcac ggtgatggac gaagatcctt
     5761 actacaaccc caacttctcg aagggaagcg gggacttcaa cctcagggcc gacctgctga
     5821 gaccgagggt actacgccct gaggtcgaac aggaatcggc agtctcgttc atgaactcgc
     5881 tgacgacacc ccgagaagaa ctcaaagagt acgtgatggc gcagcaaggg gcagccagaa
     5941 actcttcccg aaacgtgatc gtacccgcct cagaggatag gaatgggagc agcccgctac
     6001 tgaagacctt cgaacctctc gaagaccccg gtcctcaaga ggacgaacgc cccatccggt
     6061 cgttcgcggc gcccgtcgac cgtgaggcgc tccgcactga gcagttgatc tggatcttcg
     6121 gctctcccag gaccgggagt acctggctct catgcatcat ggcggagctc gacaaccaga
     6181 agcgctggca cgagccttac gtcgggctac tgttcgggtc gtttatctac gagcggctgg
     6241 gagagagcag caaactgctg aacaccaccg gcttcattat gggagaggcc cacaggaagg
     6301 tgtggctaaa ctcgataaag aacttcgtcg tcgatggggc gatggcccgg tatccgcgtt
     6361 tgaggcggaa ccagtacctg gtggtcaagg agcccaacgg ctccgtgggg gcgcccctgc
     6421 tcctcgaagc taccccggag agccgtctga tcttcctgat ccgggacccc agagacgtgg
     6481 tcgcctcgag gctggacgcg ttcagggcgg gtagctggag cgcccagcaa cgggtctaca
     6541 gtacggtggc tgaactgaac gccttcacga agcacctggc cgaggaatac gtcaatgtgg
     6601 tctcgcaggt caagagggct tacgaggcgc acgccggtag gaagacgcta attcgctacg
     6661 aagacctgag gtacgatacc tccagcactc tcgcggccat gtacgaagct cttggagtgg
     6721 agttcgacag agcgcggctc gaagccgccg tcgcgaagca cagctgggaa cagatacccc
     6781 ccgccgacaa aggtacgggg aagttctaca ggaaagcgca gccgggcggc tggaaggaag
     6841 acctgaccca ggagcaggtc tcgctcgtcg tggacgtaac gaggccgatg atccgcgagt
     6901 tctacccgcc tgacgagaat ggttacacgt tctccgggta aagctccttg agcaattggc
     6961 cggtaacctt ctcgactatt tgtacctgct cgggggacag gtcttccttc cagccacccg
     7021 gtgtcccctt gcggtagaac ttgcccctgc ccttctcgcc ctcgggcacg ttttcccacg
     7081 agtgcttctc gacggcccgc ctcagctcgt tttcgtccac agggacgcct agagttgagt
     7141 agattcgcct catctcgttc atggtgtcag acctgagatc ctcataacgg acgagcacct
     7201 tgggtcctcc gtgggcatcg tacgccagcc ttgcgctctc cacgccccgc ctgtacacct
     7261 tcgcccgctc cctgacgaag gcgtttggcc tcctgtctac cctcccgcgg cgcgtgcctg
     7321 tcttaccttc cctgctggcg taaagccagc ccccctctct ggcggcgtcg aggacggagg
     7381 cgaccacgtc cctcgggtca cgtacgagca ggatcatacg gctctcgggc agggcctcca
     7441 tgaggagcgg cgcccccacg gagccgttgg gttccttgac caccagatat tcttcagcgc
     7501 cgagacgggg gttggagtat cgcgccgcgt cgaggacgaa gttcctcacg gactctatcc
     7561 aacccttcct gatcgggtcc cccatcacga agtcggcgga gcgcaggttc tccttcggcg
     7621 ccctggcgta gaactccccg aagagcctac ccaccatggg ctcctcccag acccggaagc
     7681 cgccgatctc ccccatcatg ctgcgtagcc aggtgctccc cgaacgcccg gagccgaaga
     7741 tccagaccat cctccccggg tcgatgccgg ccctgacgct cttcgcgccg agcctggatc
     7801 tcaaccggac cagctcgagg taggctcgct cgcgctcgcc gatgagcctg ccgtagaccc
     7861 ggatcaacgt ccggttcttc ttcgcagccc gccgcatggc ccgtcccgac ccgagaacac
     7921 gcgctctcag gccctcaaac atcgtcccca cagggagccg aagctaacgt tttgcatctc
     7981 cggcctgagg ccatcttcac ttcctccgtc atagaaacgt tcgaaaagtg tgtcacacaa
     8041 cccttaactt ttctgaactc ctgtcccata ataggcttcg taactacacg acgtttgccc
     8101 tgagggggga atttgcgtca caaatggttg gtctttctgc cggttctggc cgtcataaca
     8161 atggcagcat cgggcgccgg cgtggaggca aggcaggctt cgctctccga cgagttcggt
     8221 cgtgcctcgg aggtgtacgg cgtaccgaag gagcttctcc ttgctatggg atacgtcaac
     8281 accaggtggg agatgccgcc gccggacgct agcgattacg aagcgggcga cccggagggc
     8341 cggggcaact acggggtcat ggccctggtc aagaacccct cgacggatac gctgggtgag
     8401 gccgcatccc tcacgggtct ttcggaaagg gatctcaaaa cggatcgcgc gtcgaacgtc
     8461 ctcggagggg ctgccgtcct cgcggaactg gcgggttcac agaggccatc cgacctcggt
     8521 ggctggtacg acgccgtcgg caggtacggg aacggcgcgc tctacgcgca gcaggtctac
     8581 gaggtcctga agagcggggc gtccgctgac atctccaccg gcgagcacct ggagctcgcg
     8641 ccgcaaagcg tggatgttcc cccgatctac accacgcagg cgacgggcga ctactcagag
     8701 tcaacctggt acggcaacgg gggcggcaac tacaccaact ccaaccgcga ggccacctac
     8761 gacatcaaca agatcgtcat ccacgtaacg cagggttcgt ggtcgagcgc cataaactgg
     8821 ttcgcgaact ccgacaacaa acagtcctcc gcccattaca ccgtccgctc ctccgacggg
     8881 ttcatcggcc agtccgtccg tgaggaggac atcgcctggc acgc
//
LOCUS       DATAIR010000669         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_160125, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000669 DATAIR010000000
VERSION     DATAIR010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160125"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="galK"
                     /locus_tag="VHM69_15835"
     CDS             complement(<1..1005)
                     /gene="galK"
                     /locus_tag="VHM69_15835"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX2741914.1"
                     /translation="MRETEQRASRAYMDRFGEEPVLVASAPGRVNLIGEHTDYNGGFV
                     LPCAIDRRIAVAVGAGEGGLYSADFGETRPLGEKDSTWADYPRGVVWAMGEAGYEIGA
                     FRAAFSGDVPLGSGLSSSAAIEAATALALDALFGLGIGGKDLAVLCQRAENEFVGVNS
                     GIMDQYASLLCEAGAALLVDCRSLEARGVPLDLKGADLALLVCDTRVERGLADTGYND
                     RRAACERAARAIGVEELRDATEEDLALLSGEELKRARHVVSENARVLVAVKVLEDGDF
                     DEFGRLMYASHASLRDDYEVSTPELDAFVEIAQDHGASGARLTGAGFGGCAIALVPES
                     E"
     gene            complement(1014..1985)
                     /gene="galE"
                     /locus_tag="VHM69_15840"
     CDS             complement(1014..1985)
                     /gene="galE"
                     /locus_tag="VHM69_15840"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX2741915.1"
                     /translation="MKVLVTGGAGYIGSVVASQLVEAGYETVLLDDLSKGYREAVPEG
                     TRLAIGSLLDMDFVRETLSGGFDGVLHFAALSLVGESVEQPERYYRNNVCGTLNLLEA
                     MRETGTGKLVFSSTAAVYGEPEEVPIQEIAPALPTNPYGSSKLAVDSLIGAVARSQGL
                     AATSLRYFNVAGSSGQFGEDHRPETHLIPLVLRAAADGTSVKIFGTDYPTRDGTAVRD
                     YIHVEDLGRAHLLALEAAGPGEHSVYNLGNGEGFSVREVVEAARRVTGKDIEAVDAPR
                     RAGDPPVLVASSEKIRSDLGWKPEKPELETMISDAWTWMRDHPNGYE"
     gene            complement(1982..2965)
                     /gene="galT"
                     /locus_tag="VHM69_15845"
     CDS             complement(1982..2965)
                     /gene="galT"
                     /locus_tag="VHM69_15845"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX2741916.1"
                     /translation="MNQLRWDPTQREWVAYATHRQDRTFLPPAEYCPLCPTIPGGFPT
                     EVPRESYEIAVFENKFPSFAPNAPVPDESGTSLTPTAPGRGICEVVLYSDDHDATLAT
                     MSERRMRNLIEVWTDRFEELGALDFVDYVFIFENKGEAIGVTLHHPHGQIYGYPFIPP
                     RPAKELEAASEYRARNDGRCLHCDLLSQEHEDARRILTRGEHFTAFIPFYAHFPYEAH
                     VYSHRCAPSIADLDGPERRDLASVLKRLLVGYDSLFGISLPYMMVMHQAPTDGGHYKG
                     VAHFHIEFYPPNRTADKLKYLAGSETGAGAFIMDVLPERTAETLREKIEGQ"
     gene            complement(3198..>3404)
                     /locus_tag="VHM69_15850"
     CDS             complement(3198..>3404)
                     /locus_tag="VHM69_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741917.1"
                     /translation="DFLWPVYVLFSPENLPLPPGLSMLQRAYGTDYAIVMAGGVVATV
                     PVLLVFIFAQKYVIQGVSRSGLKG"
BASE COUNT          578 a         1111 c         1089 g          626 t
ORIGIN      
        1 ctcgctttca ggtacgaggg cgatggcaca tccgccaaaa ccggcgcccg tgagacgcgc
       61 gccgctggcg ccgtgatctt gcgctatctc cacgaatgca tccagttcgg gggtcgagac
      121 ctcgtagtcg tcacgcagcg aggcgtgcga ggcgtacatc aggcgcccga actcgtcgaa
      181 gtccccgtcc tcgagcacct tgacagccac aaggacgcgg gcgttctcgg agacgacgtg
      241 cctggcgcgc ttcaactcct cgccggagag gagagccagg tcctcctccg tggcatcgcg
      301 cagctcctcc accccaatcg ctctggccgc gcgctcgcac gcagccctcc tgtcgttgta
      361 gccggtatcg gcgaggccgc gctcgacgcg ggtgtcacag acgagaaggg cgaggtcggc
      421 gcccttcagg tcgagcggta cgccccgtgc ctcaagcgag cggcagtcga cgagcagcgc
      481 ggctccggcc tcgcagagca gggaggcata ctggtccatg atgccgctgt tgacgcctac
      541 gaactcgttc tcggcccgct ggcacaggac ggcgaggtcc ttgccgccga tcccaagccc
      601 gaagagagcg tcgagggcga gcgccgtcgc ggcctcgatg gccgccgacg aggagagccc
      661 cgagccgagc ggtacgtccc ctgagaaggc ggcccggaaa gccccgatct cgtacccggc
      721 ctcccccatc gcccatacta cgccgcgcgg gtagtcggcc catgtggagt ctttctcccc
      781 caaaggacgc gtctcgccga agtctgcgga gtagagtccg ccttcgcccg ctcccacagc
      841 tacggcgatg cgtctgtcta tggcacaggg gagtacgaag ccgccgttgt agtccgtgtg
      901 ctccccaatt aggttcacgc ggcccggggc gcttgccacc agcacgggct cctcgccgaa
      961 cctgtccatg tacgctcgtg acgctctttg ctctgtctcg cgcaagcagg ctcctactcg
     1021 tagccgttcg ggtggtcacg catccaggtc caggcgtcgg agatcatcgt ttccagctct
     1081 ggcttctccg gcttccagcc gaggtcggac cgtatcttct cgctcgaagc aacgaggacg
     1141 ggcggatcac ccgcccgccg cggcgcatcg acggcttcta tgtcctttcc cgtgacccgg
     1201 cgcgcagcct caacgacctc cctgacagag aagccctctc cgttgcccag gttatagacg
     1261 ctgtgttccc caggcccggc ggcctcgagc gcgagcagat gggcgcgccc gaggtcctcg
     1321 acgtggatgt agtcgcgcac ggcggtgccg tctcgcgtcg ggtagtcggt cccgaagatc
     1381 ttcacggagg taccatccgc cgctgccctg aggacgagtg ggatcaggtg cgtctcaggc
     1441 cgatggtcct ccccgaattg cccgctggac cccgcgacgt tgaagtagcg aaggctcgtc
     1501 gcggcgagcc cttgggatcg ggcaacggca ccgatcaggc tgtcaacggc gagctttgaa
     1561 ctcccgtacg gattggtcgg caaggcaggt gctatctcct gtatcggcac ctcctcgggc
     1621 tccccataga ccgctgccgt ggacgagaag acgagctttc ccgtacccgt ctcgcgcatc
     1681 gcttcgagca ggttgagcgt accgcagacg ttattacggt agtagcgctc gggctgctct
     1741 acggactcgc cgacgaggga gagagcggcg aagtggagca ccccgtcgaa gcccccagag
     1801 agcgtctcgc ggacgaaatc catgtccaga agacttccga tcgccagccg cgtcccttcg
     1861 ggcacggcct ctcgataccc tttggagagg tcgtcgagca gcacagtctc gtagccggcc
     1921 tcgacgagct gggatgcgac gacgctccct atgtagccag cgccgccggt tacgagtacc
     1981 ttcattggcc ctctatcttt tcgcgcaggg tctcggctgt cctttcaggc agcacgtcca
     2041 tgatgaaagc gcctgccccc gtctcgctgc ccgcgaggta cttcagcttg tccgcggtac
     2101 ggttcggcgg gtagaactcg atgtggaaat gggcgacgcc cttgtagtgt ccgccgtcgg
     2161 tcggggcctg gtgcatgacc atcatataag ggagcgagat gccgaatagg gaatcgtagc
     2221 ccacgagcag ccgtttcagg acagacgcca ggtcccggcg ctccgggcca tcgaggtctg
     2281 ctatcgaggg ggcgcagcgg tgggagtaga cgtgcgcctc gtaggggaag tgggcgtaga
     2341 aagggatgaa ggccgtgaag tgctcgcccc ttgtcagtat ccggcgcgcg tcctcgtgct
     2401 cctgggagag gaggtcgcag tgcaggcagc gtccatcatt ccgtgccctg tactcgctgg
     2461 ccgcctccag ttccttcgcg gggcgcggtg ggatgaaggg gtagccgtag atctggccgt
     2521 gcgggtgatg cagcgtcacc cctatggcct cgcccttgtt ctcgaagatg aagacgtagt
     2581 caacgaagtc aagggcgccg agctcctcga agcggtcggt ccagacctcg atcaggttac
     2641 gcatacggcg ctcgctcatg gtcgcgagcg tcgcgtcgtg gtcgtctgag tagagaacga
     2701 cctcgcagat gccgcgcccc ggagccgtcg gcgtcagcga ggttccgctc tcgtcaggaa
     2761 cgggggcgtt cggggcgaac gaagggaact tgttctcgaa gacggctatc tcgtacgact
     2821 cccttggcac ctccgtaggg aagcctcccg gtatcgtcgg gcacagggga caatactccg
     2881 ccggtggaag gaacgtgcgg tcctgcctgt gggttgcgta ggcgacccac tcgcgctgag
     2941 tagggtccca gcgaagctgg ttcaatcctc tcccccctcg tcttcctcga acgtgtagaa
     3001 gatgatgtag cggccgtcgt cctttatctc gcgttccttc tgcggttcgg acttctcctg
     3061 cacgaaagta ctccttacag tgggctgatt cacgggctaa gggcgatata ccctgaagta
     3121 gttcctctcg ccctcgcgcg acggctcgct aatcccgaac aaccgtatca caccgtggcg
     3181 cttcgccgtc acactgacta gcctttcagg ccgctgcggg agacaccttg tatcacgtac
     3241 ttctgggcga agatgaagac cagcaagacg ggtacggtgg ccactacgcc accggccatc
     3301 acgatcgcgt agtctgtccc gtaggctctc tggagcatgg agagccctgg cgggagcggc
     3361 aggttctccg ggctaaagag aacatagacc ggccagagaa agtc
//
LOCUS       DATAIR010000670        11056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_206334, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000670 DATAIR010000000
VERSION     DATAIR010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206334"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VHM69_15855"
     CDS             <1..1329
                     /locus_tag="VHM69_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX2741918.1"
                     /translation="LQEDQLMERDDEQTMRRARMVLDFNWTGEYTQPGPRLYPHQWSW
                     DSALIAIAYAHYDQNRAEKELRHLFEAQWKNGLLPQIIFNSRLSGYFPGPGFWHAKES
                     PAAPEHIKTSGVVLPPVHATAVLRLHEHAEDKARARMFLEYAFPRLAAWHEYLYRERD
                     SHEEGLVYIRHPWESGMDNSPIWDVVLQRLQLRPEEIPRYNRADTHIVSTEDRPTSDD
                     YDRFAYLVKFFADRDYDERRIREECPFLVQDVLFNSLLCRSGTDLAQIARIVGEDPTP
                     FEERATKTAGAVNDKLWDEEHSTYLDFDLVEGRLIHAYAAPNFCALFAGIPDKERAGR
                     MVESLKSNFGLAVGGTPVASYDVYGFGFSPVRYWRGPVWINIDWFLMHGLRRYGYEDL
                     ADRLRQTIIDLCREQGFHEYFNPMEGSGHGSDLFSWSAALFIDVLMEEGR"
     gene            complement(1368..2306)
                     /locus_tag="VHM69_15860"
     CDS             complement(1368..2306)
                     /locus_tag="VHM69_15860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX2741919.1"
                     /translation="MRLDPAGGKKLDGGLSLAESYDLCRRVQKAHSRTYYFSTRLFPA
                     EVRPRVHALYAFMRYADEIVDTPHDLPLDAQLSVLEDFEAETMAAVSGEEVPNPVLRA
                     YADTVRTCGIDPGTITAFFESMKMDTRVFRYPTYEDLEVYTYGSAAVVGLMMCKVVGV
                     ADGKADPHAEALGVAMQLSNFLRDVGEDWRRGRVYLPLEDLARFGYTERDLASGVVDE
                     RFVDLMRFEIDRARKLYEIADEGMAYIPRGRRFPVVVARELYAAILDHIEAQRYDVFS
                     RRAQVSRPGKIVVAARCAARDPGEIAARIRYRRTGP"
     gene            complement(2303..3814)
                     /gene="crtI"
                     /locus_tag="VHM69_15865"
     CDS             complement(2303..3814)
                     /gene="crtI"
                     /locus_tag="VHM69_15865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX2741920.1"
                     /translation="MGTLAMARPKKRVAVVGAGMGGLAAAIRLAATGFEVDVFEKNAQ
                     VGGRVGRLGAEGFTFDTGPSLLLMTDTYRELFRSAGRDLDDYVRLIPLDANYRITFGD
                     GDALTIRRTLPELIKELERIEPGVTPTFYRFLEDACHKYRLGRSEFVERDFEGAGDFF
                     GLRNLRLLLKTKAVNNYYRQVSKFFQTEKLRQAFSLQTMYLGLSPFEAPAVYSLLPYT
                     ELAEDGLWFPEGGMYSLVEAMERLATELGVRFHMNSPVEEILVTKGRARGVRVSGGEI
                     GADAVLANADLPYAYRELLGGAADGDFRLRKRQKLEYTASAFMLYLGIDGNLGDMVHH
                     NFYLSGRYRENFQAIFRDHTLPDDPSFYAVVPSKTEPRMAPDGMESLFVLVPVPYLGP
                     NVDWNRDGDAFREKVYGLLERRCGIARDRVVYEKVRTPLDWRDEYNLEEGAAFGIGHG
                     IFQVGYFRPPMVSRAVDGLYFVGASTRPGTGVPLVTIGARLVADRIRREVGIY"
     gene            3900..5063
                     /locus_tag="VHM69_15870"
     CDS             3900..5063
                     /locus_tag="VHM69_15870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX2741921.1"
                     /translation="MDLPIAVLKNRGAVRVRSGHPWVYRSDVASAEGGAGDVVRVTDR
                     GGNFLGMAFLNPSSEISLRVVTREDEPIDEGWFRRKLEVALAYRSSLDIDADAYRLMH
                     AEADGVPGLVVDRYGDYVVLQIGVAAVERRMGWVISSLEDLLAPAGILLRADSLARKR
                     EGLDTEVRSLSGKVPEGVVVREGPVRYTARLWTGQKTGSFLDQRENHLAAGRFANGRV
                     LDVFSYAGGFALHAAGKAESVEAVDSSVAALEAARANAELNGLTNLTFTRKNAFDLLR
                     GRSDSGELYQTVILDPPAFAKARRDLPGAIRAYKEINLRAIKLLATGGTLITCSCSYH
                     FSRELMQDTLRSAAADVGATMRVREWRSQAADHPEVLTIPETRYLKCAVLERL"
     gene            5151..6251
                     /locus_tag="VHM69_15875"
     CDS             5151..6251
                     /locus_tag="VHM69_15875"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX2741922.1"
                     /translation="MASSRDFLSTPLCELLGIRYPIIQAPMAGGWTTPDLVCAVCEAG
                     GFGVLAGARVSPEKLREDIRTVKARTDRPFGVNFLIAPPGEGNEDVASVQRFLDRFRN
                     RLGLPPGRIDITLPPSPLEEQLEIVFEERVPVLSVALGDPGELVERAHEEGALVSAMV
                     TTVEEAVRVAERGVDIVVAQGAEAGGHRSTFEVGPRGDAPLVGTMALVPRVVDAVEVP
                     VVAAGGIADGRGLLAALALGASGVQLGTRFLVARESGAYPAYRQRLLEAVETDTVVTH
                     VFTGRPARGLRNRLAEEYLRDGPEPLGWLLHGAAAGDIYGAAQADDDGDHSPLLAGQG
                     LGLIREEQGAAEIVEQLVQEAESVLSRLAGSP"
     gene            6305..7663
                     /locus_tag="VHM69_15880"
     CDS             6305..7663
                     /locus_tag="VHM69_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2741923.1"
                     /translation="MEGRGSRRVWLVIAVLLFGGFLVAGRLVPAVRWFFRQRVERVVE
                     EVGSRGVKLPAFKLTRRRALIHHLTHDPELIGAAEVYARENGISMAAAMARVERYARE
                     IVPSFNAFLYFRIGIPLAGWISRSLYDVRVDSERGLAALDESPSGVASVVFVMNHRSN
                     IDYVLLAHLMAHRTALSYAAGEWACVWPLGRLIGGMGAFFVRRGSGDDLYRRVLERFV
                     QMAVEGGLTQVFFPEGGLSRDGKPREPKIGLLDYMLRRFDPRACDILFVPVGVNYDWV
                     LEDHSLLQTGGPEAGIRGRGGLFASAAASIVRNLMQARRGGSFHLGGAAVGVGSPISA
                     REYAASRGIEFRELEREDRIEEVKVLAELLMETINESIPDTAAPLLARALLEAPERAV
                     PEEKLIANARTLAEGAGRLDYEGALRKLAARGLVLAERGGYRPATGSEKLLGYYAGSS
                     YR"
     gene            complement(7675..9153)
                     /locus_tag="VHM69_15885"
     CDS             complement(7675..9153)
                     /locus_tag="VHM69_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741924.1"
                     /translation="MSARRSEKSNLADSAVLESITSQWWSFSSFLPLLTGALWLWLAA
                     RAGLPALLLGAVPGALLLGTGLSGLLWAVESRTFQYMALASSLGAILSLPAILLLGPL
                     AALVLGAGSAASFLATGYLALGQHRCPAGVPVPDTGPGLAARAAVNELMMCGLILTSW
                     PVVVGSIAARVQREAIDAYALFEEKGWFSDPATYHEDPPPLEEPKIESIEKRGRTIEQ
                     LTFESLYEPHEDEPGRERWLSYERNPTAWAWILRHSGEPRPWLICVHGIRMGTLNKSL
                     IRFQPEYLHEELGLNLIMPVLPMHGPRDTGGLISGERTLSGDVMDTLHTGAQAMWDLR
                     QLVRWLRESEGAPAVGALGHSLGGYAVSLLASLEEDLDCVVAGNPAVDPAHLFWNNAL
                     ALATHSLSAEGIREETFTALLRPVSPLALEAVVPHERRAIFAGVVDRVVPPVQAHSLW
                     RHWEEPRIAWYQGAHQRFIRSSEGRKVLEDTLRAADMLRKDG"
     gene            9369..10289
                     /locus_tag="VHM69_15890"
     CDS             9369..10289
                     /locus_tag="VHM69_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741925.1"
                     /translation="MRNREVVMQVKVSRRLVAVAAMIFAFAPGAVGTVFAQDTTTING
                     CVNKKSGALRVVSDPSACKSGETPISWNQQGPPGADGAVGTDGQDGKTILNGSGTPAA
                     DLGSEGDFYIDTSANNIYGPKSADGWGSPTSLVGPAGSGGDADTLDSKDSTEFAAADH
                     QHVTRIQQFGGQVSNELFATPTQWSFAGPTATVTVSNGQRITGSAQAPLGLTYDTSDD
                     LLYGLCYKSTDAESFVIPFTYYGSSDTESPSRAEVSTTRIPFAASETISGLTAGTYNV
                     GFCVRAAAPDGFLVLDDNGYVNGWVMVTND"
     gene            complement(10630..>11056)
                     /locus_tag="VHM69_15895"
     CDS             complement(10630..>11056)
                     /locus_tag="VHM69_15895"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX2741926.1"
                     /translation="AQIESDFNRQYVFFYAIMGVSVAVSAFGVVNTLSMIVFERTREI
                     GILRAVGTTRLQIGRLIIDEGIVISLIGCLVGVALGSLLGYLFVQGSGAGGFEIDFYY
                     PKLPALAALLSGLFIGVFAGLLPARSAARKDIVEAVQYE"
BASE COUNT         2042 a         3452 c         3594 g         1968 t
ORIGIN      
        1 ctccaggagg accaattaat ggagcgagac gacgagcaaa cgatgcggcg ggccaggatg
       61 gtcctggact tcaactggac gggcgaatac acccaaccgg gacccaggct gtatccgcac
      121 cagtggtcgt gggattccgc gctaatcgcc atcgcctacg cccactacga tcagaacagg
      181 gctgagaagg agttgcggca tctcttcgaa gcgcagtgga agaatgggct gctgccccag
      241 atcatcttca actccaggtt atccggctat ttccctggtc ctggattctg gcacgcgaag
      301 gagagccccg ccgctccaga acacatcaag acctcgggcg tggtcctgcc gcccgtccac
      361 gcgacggcgg tactgcgcct ccacgaacat gcggaagaca aagctagggc gaggatgttc
      421 ctggagtacg ccttccccag gctggcggcc tggcacgagt acctctaccg cgagcgcgac
      481 tcgcacgaag agggcctggt gtatatccgg cacccgtggg agagcgggat ggacaactcg
      541 ccgatatggg atgtcgtcct gcagcggctc caactgaggc cggaggagat ccctcggtat
      601 aaccgggccg acacccacat cgtctctacc gaggaccgtc cgaccagcga cgactacgat
      661 cgtttcgcct acctcgtcaa gttcttcgcc gaccgcgact acgacgagcg acgcatccgt
      721 gaggagtgcc cgtttctggt gcaggacgtg ctcttcaatt ccctgctgtg caggtcaggt
      781 acggacctcg ctcagatagc gcggatcgtc ggcgaagacc cgacgccgtt cgaggagagg
      841 gccacaaaga ctgctggtgc cgtaaacgac aagctctggg acgaggagca cagcacatac
      901 ctggacttcg acctggtcga gggcaggctt atccatgcgt acgccgcgcc caacttctgc
      961 gcgctgttcg ccggcatacc ggacaaggag cgggccggac gcatggtcga gagcctcaag
     1021 agtaacttcg gcctggcagt ggggggaacg cccgtggcca gctacgacgt gtacggcttc
     1081 gggttctcgc cggtcaggta ctggcggggt ccggtctgga tcaacataga ctggttcttg
     1141 atgcatgggc tcaggcgtta cggctacgag gatctggctg atcgtctgcg acagacaatc
     1201 atagatctgt gcagggaaca gggcttccac gagtacttca acccgatgga aggatccggg
     1261 cacggctccg acctcttctc gtggtcggca gccctgttca tagacgtgct gatggaagaa
     1321 gggaggtagg ttatgtaacc gcagaacggg gcccggtccc tattgggcta cgggccggtc
     1381 cgcctgtagc ggatgcgggc cgctatctcg ccaggatccc tggccgcgca cctggcagcg
     1441 accactattt tcccggggcg cgagacctgc gccctgcgcg agaagacatc gtacctttgg
     1501 gcctcgatgt ggtccaggat agccgcgtac aattcgcgcg ccacgaccac ggggaagcgt
     1561 ctccccctcg ggatgtatgc catcccctcg tcggcgatct cgtagagctt cctcgccctg
     1621 tctatctcga agcgcatgag atcgacgaac cgctcgtcga ccacaccgga tgcgagatcg
     1681 cgctccgtat acccgaagcg cgccagatcc tccaatggca aatacacccg cccgcgtcgc
     1741 cagtcctccc caacgtcgcg caggaagttg gagagctgca tggcgacgcc gagggcctcg
     1801 gcatggggat cagccttccc gtcggccaca ccgacgacct tgcacatcat caggcctacg
     1861 acggcagcgc tcccgtaggt gtagacctcg aggtcctcat acgtcggata acggaagaca
     1921 cgggtgtcca tcttcatgct ttcgaagaag gcggtaatcg tccccgggtc gatgccgcag
     1981 gtgcgtaccg tgtcggcgta ggcgcgcagg acggggttgg ggacttcctc acctgagacg
     2041 gctgccatcg tctcggcctc gaaatcttcg aggacggaga gctgggcgtc caggggcagg
     2101 tcgtgggggg tgtccacgat ctcgtcggcg taacgcatga aagcgtagag ggcgtgcacg
     2161 cgcggcctga cctcagcggg gaagagacgg gtcgagaagt aatatgttct gctgtgcgcc
     2221 ttctgtaccc tgcggcacag atcgtagctc tcggcgagcg agagcccgcc gtcgagtttc
     2281 ttgccgccag cagggtcgag tctcaatata ttccgacctc gcgcctgatg cggtccgcca
     2341 cgagcctcgc gcctattgtc accagcggta ccccggtccc cggcctggta ctcgcgccga
     2401 cgaagtacag gccgtccacg gctctcgaaa ccatgggagg tcggaagtag ccgacctgga
     2461 agatgccgtg cccgatcccg aacgcagcac cctcctccag gttgtactcg tcccgccagt
     2521 cgagcggcgt cctgaccttc tcgtagacga cacgatcccg cgctatgccg caccgccgtt
     2581 ccagcagccc gtataccttc tccctgaacg catccccgtc gcggttccag tccacgttcg
     2641 ggccgagata cggcacgggt acgagcacga acaggctctc catcccgtca ggggccatgc
     2701 gcggctccgt cttcgagggg acgacggcgt agaacgaggg gtcatcgggc agcgtgtgat
     2761 ccctgaaaat agcctggaag ttctccctgt agcgccccga gaggtagaag ttgtggtgca
     2821 ccatgtcccc gagattcccg tctattccca gatagagcat gaacgctgag gccgtgtact
     2881 ccagcttctg gcgtttcctg agcctgaaat ccccatccgc ggccccgccc agtagctcgc
     2941 ggtaggcgta cggaaggtcg gcgttcgcga gcacggcgtc ggcccctatc tcgccaccac
     3001 tcaccctgac gccgcgtgcc cttcccttcg ttaccaggat ctcctcgacg ggactgttca
     3061 tgtggaacct cacgccaagc tcggtcgcga gtctctccat cgcttcgacg agggagtaca
     3121 tcccgccctc ggggaaccag agtccgtcct cggccagttc tgtgtaaggg aggagcgaat
     3181 agaccgccgg cgcctcgaag ggtgagaggc cgaggtacat cgtctgcagg gagaaggcct
     3241 gcctgagctt ttcggtctgg aagaacttcg agacctggcg gtagtaattg ttgaccgctt
     3301 tcgtcttcag gaggaggcgg aggttgcgga gtccgaagaa gtccccggca ccctcgaagt
     3361 cgcgctcgac gaactccgag cgtccgaggc ggtacttgtg gcaggcgtct tccaggaagc
     3421 ggtagaaggt cggggtcacg cccggctcga tgcgttccag ttcctttatc agttcaggga
     3481 gggtgcgccg gatcgtcagg gcgtcgccgt cgccgaaggt gatgcggtag ttggcgtcga
     3541 gcggtatgag cctgacatag tcgtcgaggt cgcgccctgc ggagcggaag agctcgcggt
     3601 aagtgtccgt catcaggagg agggatgggc ccgtatcgaa ggtgaagcct tcggccccga
     3661 gccttccaac gcgtcccccg acctgcgcgt tcttctcgaa gacatctact tcgaagcccg
     3721 tggctgccag ccggatcgcc gccgcaaggc cacccatccc ggccccgaca accgccaccc
     3781 gcttcttcgg cctggccatc gccagagtgc ccacctactt tcaagggtat atagagagta
     3841 tacgttgcag atgctaacag cgttcaccac gcgcacgggt aaaggagctt acaaggatct
     3901 tggatcttcc gatagccgtc ctgaaaaaca ggggcgcggt gcgcgtccgc tccggacacc
     3961 cctgggtcta ccgctccgac gtggcgagcg ccgaaggcgg ggccggagac gtggtgcgcg
     4021 tgaccgaccg gggcggcaac ttcctcggca tggctttcct caacccgagc agtgagatct
     4081 cactccgcgt agtaacccgc gaggacgagc cgatcgacga aggctggttc cgtcgaaagc
     4141 tcgaagtcgc tctcgcctac cgttcttccc tcgatataga cgccgacgcc taccggctca
     4201 tgcacgccga ggctgacggc gttcccggcc tcgtcgttga ccgctacggc gactacgtcg
     4261 tcctccagat cggcgtcgcc gccgtggagc ggcgcatggg gtgggtgatc tctagcctcg
     4321 aagacctgct cgcgcccgcg gggatcctcc ttcgagccga ctctctcgcc cgcaagcgcg
     4381 agggcctgga tacggaggtg cgctctctct ctggcaaagt ccccgagggc gtcgtcgtaa
     4441 gggaggggcc ggtgcgctac acggcccgcc tgtggaccgg gcagaagacg ggctccttcc
     4501 tcgaccagcg cgagaaccat ctcgcagccg gtcgattcgc aaatggccgg gtcctcgacg
     4561 tcttctccta cgcgggcggc ttcgcgctgc acgccgccgg caaggcagaa tcggtcgaag
     4621 ccgtggactc gagcgtggcc gcgctcgaag ccgcccgcgc caacgccgaa ctcaacggcc
     4681 tcaccaacct gaccttcacc cgcaagaacg ccttcgactt gctgcgtggg cgctccgact
     4741 ccggggagct ctaccagacc gttattctcg accctcccgc gttcgccaag gccaggcgag
     4801 atcttccggg tgcgatccgg gcctacaagg agatcaacct gcgcgcgata aagctcctcg
     4861 ccaccggcgg caccctcatc acctgctctt gctcctacca tttctcgcgc gagctgatgc
     4921 aggacaccct gcgctccgcc gccgccgatg taggagcgac gatgcgcgtt cgggagtgga
     4981 gatctcaggc cgccgaccac cccgaagtcc tgacgatccc cgagacacgc tacctcaagt
     5041 gcgccgtgct ggagcgtctg taagtagccg acgactttcg tgtagcggaa tcgggcgtta
     5101 tattggtcct ccgggactcg cacgcaggtc agttaccgag gaggtatatc atggcgtcat
     5161 cgcgcgactt cctttcgacc ccgctctgtg agttgctcgg gattcgctac cccatcatcc
     5221 aggcgccgat ggcagggggg tggacgacgc ctgatctcgt ctgcgccgta tgcgaggcgg
     5281 gagggttcgg ggtgctcgcg ggtgcgcgcg tttcgcccga gaagcttcgg gaagacatca
     5341 ggacggtcaa ggcgcgtacg gacaggccat tcggggtcaa cttcctgatc gcgcctccag
     5401 gagaggggaa cgaggacgtg gcatcggtcc agcgtttcct cgatcgcttc aggaatagac
     5461 tgggcctgcc accgggacgc atcgacatta ccctgccgcc ttcgccgctg gaggagcaac
     5521 tggagatcgt cttcgaggag agggtgcctg tattgagcgt ggccctggga gacccgggag
     5581 agctcgtaga acgggcacac gaggaaggcg ctctggtttc ggctatggtc actacggtcg
     5641 aagaagccgt gcgcgtggcg gagcgcggcg tggacatcgt cgttgcccag ggggcggagg
     5701 ccggaggaca ccgctctacc ttcgaggtcg gtccccgtgg agacgcgccg ctcgtcggta
     5761 cgatggcgct ggtgccgcgg gtggttgacg cggtggaagt cccggtggtc gccgcgggcg
     5821 gcatcgcgga cggacggggt ctactcgccg cgctcgcgct cggggcttcg ggggttcagc
     5881 tcggaacccg ctttctcgtg gcgcgggaga gcggggccta tcccgcgtac cggcagcgtc
     5941 tgctcgaagc cgtcgaaacg gacacggtcg tcacgcacgt ctttaccgga cgtccggcgc
     6001 gtggcctccg caaccggctg gccgaggagt accttcgcga cggtcccgag ccgcttggct
     6061 ggctgcttca cggcgcggcg gcgggagaca tctacggcgc cgcgcaggcc gacgacgacg
     6121 gggatcactc accgctgctg gcgggacagg ggctcgggct aatccgagaa gagcaggggg
     6181 cggccgagat cgtcgaacaa ctcgtgcagg aagcagagag cgtgctgtcg cgcctggccg
     6241 gtagcccgta aagagtggtg ggctatactg cgttctcggg gtctggtctg acggggagag
     6301 cgtaatggaa ggtaggggta gcaggcgagt ttggctggtc atcgccgtgc tgttgttcgg
     6361 tgggttcctg gtagcgggac ggctcgttcc cgccgtccgc tggttcttca ggcagagggt
     6421 cgagagggtc gttgaagagg tggggagcag gggtgtcaag ctgcccgcgt tcaagctgac
     6481 ccgccgcagg gctttgatcc accacctcac ccacgacccc gagttaatcg gggccgccga
     6541 agtctatgct cgtgagaacg gcatctcgat ggcggccgcg atggccaggg tcgagcgata
     6601 tgcccgtgag atagtacctt cattcaacgc cttcctttac ttcaggatcg ggatccctct
     6661 ggcgggttgg atctccaggt ctctctacga cgtccgcgtc gactcggaga gaggactcgc
     6721 ggcgctggac gagagcccga gcggggtcgc gagcgtggtc ttcgtgatga accacaggag
     6781 caacatcgac tacgtgctcc tggcacacct gatggcgcat cgcaccgccc tcagctacgc
     6841 ggcgggggag tgggcttgcg tctggcccct ggggcgtctg atcgggggca tgggcgcgtt
     6901 cttcgtgcgc cgtggttctg gcgatgatct ctacaggcgg gtgctggagc gcttcgtcca
     6961 gatggccgtc gagggcggcc ttacgcaggt tttcttcccc gagggcggac tgagccggga
     7021 cggaaaacca cgcgagccga aaataggtct cctcgactac atgctcaggc gtttcgaccc
     7081 acgagcctgc gacatcctct tcgtgcccgt cggcgtcaac tacgattggg tgctcgaaga
     7141 ccacagcctc ctgcagacag gtggaccgga ggccggtatt cggggacgcg gaggactctt
     7201 cgcctcggcg gcagcctcca tagtcagaaa cctcatgcaa gcacggcgtg gaggcagctt
     7261 ccatctcggc ggcgccgccg taggggtcgg gtcgcctatc tccgcgcgcg agtacgccgc
     7321 atcgcgcggg atcgagttcc gagagctgga gcgggaagac aggatcgaag aggtcaaagt
     7381 cctggccgaa ctcctcatgg aaaccataaa cgagtccatc ccagatactg ccgcaccgct
     7441 tctcgcccgc gccctgctcg aagcgccgga gagagccgta cctgaagaga agttgatcgc
     7501 caacgcccga actctcgcgg agggagcagg gcggctcgac tacgagggcg ccttgcggaa
     7561 gctggcggcg cgggggctgg tgctcgcgga gcgtggcgga taccgccccg cgacggggag
     7621 cgagaagcta cttggctact atgccggatc ttcctaccgt taacaggtgt gccgctagcc
     7681 gtccttccga agcatgtccg cggcacggag ggtatcttcc aggaccttgc ggccttccga
     7741 ggaacgtata aagcgctggt gtgctccctg gtaccaggcg attcgcggtt cttcccagtg
     7801 gcgccacagg ctgtgggcct gcaccggagg cacgacccga tctacgacgc cggcgaatat
     7861 cgccctgcgc tcgtgcggga caacggcctc cagcgcgagc ggagagacgg ggcgaagcaa
     7921 tgccgtgaac gtctcctcac ggatgccctc ggcgctgagc gagtgggttg cgagggcgag
     7981 agcgttgttc cagaataggt gtgcagggtc caccgccggg ttgcctgcga ccacgcagtc
     8041 cagatcttcc tccagcgagg ccagcagcga gacggcatag ccgcccagcg agtgacctag
     8101 cgccccaacg gccggcgccc cctcgctctc tcgcagccag cgcacgagct gcctgaggtc
     8161 ccacatagcc tgagcgcccg tgtgcagcgt atccataacg tctcccgaga gcgttcgttc
     8221 tccgctgata aggccgccgg tatcgcgtgg gccgtgcatg gggagaacgg gcatgatcag
     8281 gttgagcccg agctcctcgt gcaggtattc cggctggaag cggatcaagc tcttgttgag
     8341 ggtgcccatc cttattccgt ggacgcagat cagccacgga cgcggctcac ctgagtggcg
     8401 caggatccag gcccacgccg tcgggttacg ttcgtatgag agccaccgct cgcggcctgg
     8461 ctcgtcctcg tgcggttcgt agaggctttc gaaggtgagt tgctcgatcg tccttcccct
     8521 cttttcgatg ctttcgattt tgggttcctc caggggcggc gggtcctcgt ggtaggtggc
     8581 gggatctgag aaccagccct tctcctcgaa cagagcgtat gcgtctatgg cctcgcgctg
     8641 gacgcgggcg gcgatcgagc ccaccaccac gggccagctc gtgagaatga ggccgcacat
     8701 catgagctcg tttaccgccg cccttgcagc gagccccggc cccgtgtcgg ggaccggcac
     8761 tcctgcggga caacgatgct gtccgagggc caggtagccc gtcgcgagaa agctcgccgc
     8821 agaacctgct ccgagtacga gcgcggccag gggcccgagg agcaggatcg ccggcagaga
     8881 gagtattgct ccgaggctgg aggcaagcgc catgtactgg aacgtcctgg attcgaccgc
     8941 ccacagaagt cccgaaagcc ccgtgccgag gagcaacgct cccggtaccg ccccgagcaa
     9001 aagcgcaggc agaccggcgc gcgccgccag ccagagccag agcgcacccg tgagtaacgg
     9061 aagaaaggac gagaaagacc accactgcga tgttatggac tccagaaccg cactgtcagc
     9121 gaggttgctc ttctccgatc gccttgcgct catcaaccaa cccttctcaa gcaccgggag
     9181 acacgatcga tccattctag acgttgacac cactcgtggc gacgctggaa ttcctgaaag
     9241 atggaacttg ctaccgttca tgtaccgcat gctgatcgcc gaactatacc gtggcgacaa
     9301 actgccatgg tcgtgtgatc tggagcaggg agtgcgtggc cacccttcgc tggaaaggag
     9361 tgggaggtat gaggaacagg gaagtcgtga tgcaagtgaa ggtgagcagg aggctggtcg
     9421 cggtggcggc catgatcttc gccttcgcgc ccggggcggt aggtacggta ttcgcgcagg
     9481 atacgacgac cataaacggc tgcgtcaaca agaagtcggg ggcactcaga gtagtcagcg
     9541 acccgagcgc ctgcaagagt ggggagacgc cgatctcctg gaaccagcag ggaccaccag
     9601 gcgcagacgg cgcggttggt accgacggcc aggacggaaa aaccatcctc aacggcagcg
     9661 ggacgcccgc cgccgacctc ggctcggagg gagacttcta cattgatacc tccgccaata
     9721 acatctacgg tcccaagagc gcggacggtt ggggttcgcc gacctcgctg gtcgggcctg
     9781 ccgggtcggg cggtgacgcc gacacgctcg atagtaaaga ctcgaccgag ttcgcggcag
     9841 cggaccacca gcatgttacc cggatccagc agttcggggg ccaggtcagc aacgagctct
     9901 tcgccactcc tacgcaatgg tccttcgctg ggcccacagc gaccgttacg gtgagcaacg
     9961 gacagagaat cacaggttcg gcgcaagctc cgctcggcct cacctacgac acttcggacg
    10021 atctcttgta cgggctatgc tacaagtcca ctgacgccga atcatttgtc atcccgttca
    10081 catactacgg ttcctccgat acagagagtc cctccagagc agaggtctcc acgaccagga
    10141 taccgtttgc ggcaagcgaa acgatatccg gcctgacagc gggaacctac aatgtgggat
    10201 tctgtgtgcg tgcggctgcc ccagacggtt ttctcgtcct cgatgacaat ggttacgtca
    10261 atggttgggt aatggtgacc aacgattagg tcaacctccg tgcggcaaga gggtcggacg
    10321 atggcgggag cagatcccga agtccggccc tctgaagcat atcgaggctc gtgaacaatg
    10381 aagaacgcgt ttcccacgag cttgcggatc gggcgctcaa tgagttgctg tcggcctagc
    10441 gctctgctcg gctacctgtt tcttcggcaa tgtattcaat gccggttaca ttacccggct
    10501 gaccttacag cgcaccgcgg gagatcactg ctaggtaggg acggtaatct cctcctacaa
    10561 acgataggag caggcttatg ggggcggatg gagaattcgc gaaccttctc tcgtaacgaa
    10621 gtctcgcggc tactcgtact gaacggcctc gacgatgtcc ttgcgggcgg cggagcgggc
    10681 tggcaagagg cctgcgaaga ccccgataaa gaggccggag aggagggcgg cgagggctgg
    10741 tagcttgggg taatagaagt cgatctcgaa gccccctgcg cccgagccct ggacgaacag
    10801 gtagccgagc agagagccga gtgccacgcc tacgaggcag ccgatcaggc ttataactat
    10861 tccctcgtca attatgaggc gcccgatctg gagccgcgtc gtccctacgg ccctgaggat
    10921 gccgatctcg cgggtacgct cgaagacgat catggagagg gtgttgacga ccccgaaggc
    10981 cgagaccgcc acggagacgc ccatgatggc gtagaagaag acgtactggc ggttgaagtc
    11041 gctctctatc tgcgct
//
LOCUS       DATAIR010000671         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_344458, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000671 DATAIR010000000
VERSION     DATAIR010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344458"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..480)
                     /locus_tag="VHM69_15900"
     CDS             complement(61..480)
                     /locus_tag="VHM69_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX2741927.1"
                     /translation="MEDFTPVSGLVGGLLIGLAVTLMLLLNGRIAGISGIVGGLLAPK
                     GGDRGWRVAFVAGLLLGALASILAIGGPAPVDVLASPPAILAGGLLVGFGTRLGSGCT
                     SGHGLCGMARLSRRSIVATAVFFGVAMFTVFLTRHVV"
     gene            complement(544..1029)
                     /locus_tag="VHM69_15905"
     CDS             complement(544..1029)
                     /locus_tag="VHM69_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX2741928.1"
                     /translation="MKARPATRRDADSIVRIYNEGIEERIATFETRPRKTEDIQAWFD
                     GTHPIVVVEEGAVISFANTSAYSTRECYFGVAEFSVYTASEARGRGAGTLAMKALMEA
                     AEEAGFWKLVSRVFVENTPSRELLLSLGFRDVGIHEKHARLDGIWRDVVVVERLIQAN
                     L"
     gene            1214..2725
                     /locus_tag="VHM69_15910"
     CDS             1214..2725
                     /locus_tag="VHM69_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741929.1"
                     /translation="MPESIDVPVEPEVFRRLDRARFLRLLGAGAGLSMLPTSLAFLAG
                     PAGAQAAASGLPISGEDYPIGIWWPPPPELPPDPPDKTREEYYAEIMAAGFNFVIGGN
                     GVVNDAHNPPALQAASANGLMFLLTDARLRNLIRDGGGATQTSSLEKSTPSVMQSILG
                     RPGPKLRTAAVSDDDVKQRVQELVANYGGHPALAGINLYDEPKASMFGLVNTARTELQ
                     TSAPDELPYVNVWPSYAAAGALGTRDYRTYLERFFTGVNPPLLCFDHYPLLKKGITSD
                     YFYNWAMIRTFALDFGVPSWGFVQSVGFDGSDVGLADRRRPNRQEILWQVNVGLAYGA
                     KGIQYFTYWTPEAQPAPSIRFGQAMISTTGQPTVLYDYVREVNTYLKVVGKVLLPLFS
                     ESVVHARERRMPMGAKPFRGDRYVRDVSGSPVILGRFRDAGTSARYMLVVNRSFANRA
                     RTRLTMGGAVSRVSKLNISTGVFDKVRLQGRPRRDLVSTIQPGGARLYLLQNA"
BASE COUNT          542 a          852 c          849 g          506 t
ORIGIN      
        1 cgagaccgaa gacgagacca cagcccaggg cggcgagagt tctcagagca ccggaggcca
       61 tcagacgacg tggcgggtca ggaacacggt gaacatcgcc accccgaaga acaccgctgt
      121 ggcgacgatc gagcgccggg acaggcgcgc catgccgcac agcccgtggc cgctagtgca
      181 gcccgagcca aggcgggtgc cgaagccgac gaggagcccc cctgccagga tggccggggg
      241 agatgcaagc acgtccacgg gtgcaggtcc tcctatcgcc aggatagatg ccagggctcc
      301 gaggaggaga cccgcgacga aagccacgcg ccagcctctg tccccgccct tcggcgcgag
      361 cagtccgccg acgatgccgc tgatcccggc gatgcgccca ttaagcagca gcataagcgt
      421 gaccgcgagc ccgatcaaga gcccgcccac gagcccgctt accggcgtaa agtcctccac
      481 tcattcctcc gttcgtcgtc atcagggtag gttggatcgc gtgaggatag cacgacctgg
      541 gaactacagg ttggcctgga tgagccgctc gacgactacg acgtcgcgcc agatcccatc
      601 gagacgggcg tgtttttcgt gtattcccac atcccggaaa cccaaggaaa ggagtagctc
      661 ccggcttggc gtgttctcca cgaagacgcg cgagacgagc ttccagaagc ctgcctcttc
      721 cgccgcttcc atgagggctt tcatcgccag ggtgcccgcg ccacgtcctc gcgcctcact
      781 ggcggtatac acggagaatt cggccacgcc gaagtaacat tcgcgggtac tgtaggcgga
      841 ggtgttggcg aaggagatca ctgctccctc ctccacgact acgatgggat gcgtgccgtc
      901 gaaccaggcc tgaatgtcct ctgtcttgcg aggacgcgtc tcgaacgtcg ctatgcgctc
      961 ttcgatgccc tcgttgtaga tgcggactat ggaatccgcg tctctcctcg tggccggcct
     1021 ggctttcatg aggacatcct agcaaagttg cctcccaagg cttctattct tagagaccag
     1081 acgatgctaa tatagcaggg caattacaag gccagcctgt atcgagtact ggagaggggt
     1141 tacatggcgc gtggagaggc tcgtcgttgc atgctacgcg tcatagatat ggaccctggg
     1201 gaggaagaag tccgtgccgg aaagtattga cgttccagtg gagcccgagg tgttcaggag
     1261 gctggatcgg gcgcgcttcc tgcgcctgct cggggcgggt gcaggtcttt cgatgttacc
     1321 cacctcactc gcttttctgg ctggtccggc aggtgctcag gctgccgcgt ccggactgcc
     1381 gatctctggt gaggattacc cgatcgggat ctggtggccg cctcctcccg aactgcctcc
     1441 agaccctccg gacaagacac gcgaggaata ctacgctgag atcatggccg cgggcttcaa
     1501 cttcgttatc ggcggaaacg gcgtggtcaa cgacgcacac aacccgccgg cgcttcaagc
     1561 ggcctcggcg aacggtctta tgtttctact cacggacgca aggctcagga acctcatccg
     1621 cgacggtggt ggagctaccc agacaagttc cctggagaag agtacgccga gcgtcatgca
     1681 gtccatcctg gggcgacccg ggccgaagtt gcgtaccgcc gcggtgtctg acgacgacgt
     1741 gaagcagagg gtacaggagc ttgtggccaa ctatggggga catcctgctc tggccgggat
     1801 caacctctac gacgagccga aggcttccat gttcgggctg gtaaataccg ccaggacgga
     1861 actgcaaacg tccgccccgg acgagctccc ttacgtcaat gtatggcctt cgtacgcggc
     1921 ggcgggcgcg ctcggaacca gggattacag gacgtatctg gaacgtttct tcaccggggt
     1981 aaaccctccg cttctctgct tcgatcacta cccgctcttg aagaagggca ttacctctga
     2041 ctacttctat aactgggcga tgatccgtac cttcgcgctc gacttcggcg tcccttcatg
     2101 gggctttgtc cagagcgtcg ggttcgacgg cagcgatgtg ggcctcgccg accgccgccg
     2161 gcccaacagg caagagatcc tgtggcaggt caacgtcggc ctcgcatacg gtgcgaaggg
     2221 gatacagtac tttacgtact ggaccccgga ggcgcagccc gccccttcca tacggttcgg
     2281 gcaggccatg atctccacta ccgggcaacc aaccgtattg tacgactacg taagggaagt
     2341 caacacctac ctgaaggttg tcggcaaagt acttctgccg ctcttctcag agtccgtggt
     2401 ccacgcccgt gagaggcgga tgccgatggg agcgaaacct ttcagagggg accgatacgt
     2461 gagagatgtc agcgggagtc cggtcatcct tggcaggttc cgggatgccg gtacctcggc
     2521 caggtacatg ctggtcgtga accgctcgtt cgccaacagg gccagaacca ggctgacgat
     2581 gggcggcgcg gtgagcaggg tctcaaagct gaacatatct accggcgtgt tcgacaaggt
     2641 ccggcttcaa ggcaggccgc ggcgggacct cgtttcgacc atccagccgg gaggagccag
     2701 actctacctt ctgcagaacg cctgatccgg ctacatcttc aggtccacc
//
LOCUS       DATAIR010000672         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_344567, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000672 DATAIR010000000
VERSION     DATAIR010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344567"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VHM69_15915"
     CDS             <1..1080
                     /locus_tag="VHM69_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX2741930.1"
                     /translation="GQGTRLRPVTYDIPKALVPLRNRPFMGYMLDFLRNGGLNGAVLS
                     LGYLPDPIQAYLCGQQDLEGFSVDYAVEDRALGTAGGIKNAQEYLDGDTFVVVNGDVL
                     CGMDLAKALEIHRASDALATIVLTSVEDPTAYGLVEVDHEMLVHRFIEKPAADEVTTN
                     LVNAGVYVLEPEILDMIPAGREVSIEREIFPELQDSGRLRAHISSSYWRDIGTPRSYL
                     AASHDVLSGAVGARDGFEYLDVDPSVELGKNVRLLPPVSIGRGCRISNLATIGGRSSL
                     GNGCVIGEGAVVEGSIVLDGAEVEAGAVVRGSVIGPRARVGNNAIVRGLSVLGAGCVV
                     GEGNVLDQGIRVNPGVVVSPRSLSF"
     regulatory      1172..1247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1439..4507
                     /locus_tag="VHM69_15920"
     CDS             1439..4507
                     /locus_tag="VHM69_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2741931.1"
                     /translation="MSQIREFGPKAGLRAWLVYLVIGVAATSIFFLLPGVAQAILYNL
                     IGASMVIAILVGVRLNRPEPALPWYVIAFGLALFVSADVIYYNIYPNVLGVPAPFPSV
                     ADAFYASSYLVVAFGLAVLIRGVGGREDWGSLIDAGIIAAGLGLLAWEFLIQNYLEAA
                     SIPLFVRLVAIDYPLMGVVWVALAIRLLFMSRPPRPPALYLLVLAAAFHPIGDAIYAW
                     LVLRDAYQSGTVLDTFWMLSYVTFGAAALHPSMRELNEATRDRVTGLSGWRLTLLTAA
                     ALMVPGVLVLQTTLNEAIDVLAIAAAATVLFSLVLLRVAGLLRENERTAAELGRLNER
                     LEERVAERTADLEEAREIAESANRAKSEFLANMSHEIRTPMNGVIGMTGLMLGTDLSP
                     EQREYAETVRSSGENLLTIINDILDFSKIEAGKMDLEIIDFDLRSAVEESVGLLAERA
                     HDKGLELASLVKYDVPTTLRGDPGRIRQVLVNLLGNAVKFTEKGEVTLIVGLVEDTED
                     AAIVRFEVRDTGIGMTPEQKERLFRSFSQADASTTRRYGGTGLGLAISKQLVELMGGE
                     IRVQSEPGVGSSFFFTLPLRKQRAGASSTPKFLTDLRGLRVLIVDDNETNRKIVHHQI
                     VSWGMKNGRAEDGPSALQMLRSAKESGKPYDLVILDMQMPGMDGLELARKIKTEPLLS
                     STKLIMLSSIGRRGEGEEARQASIEAYLTKPVRQSRLFDAIALVMGTAAEETVAPEEE
                     KQRQLVTRHSLKEAKDRSRIRILVAEDNQVNQKVAVQMLKRLGYQADVAADGLEVLET
                     LSRIRYAAILMDVQMPEMDGYEATAEIRRREEGQDRHTPVIAMTANAMQGDREKALEA
                     GMDDYVPKPVKPEELKAVLGRWIAQREVTLGADTHATAAGADPTASDDEQAPLDESVL
                     EGLRELQAEGEPDILEELIEMFLEEVPPQLVAMREAIEDRDASTVEHLAHMLKGSSGN
                     MGALRMAPICGELEEVGAAEDLVRAQRLLEQLEVEFGRVRLALEAERSAN"
     gene            4600..5283
                     /locus_tag="VHM69_15925"
     CDS             4600..5283
                     /locus_tag="VHM69_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX2741932.1"
                     /translation="MYEPYASALADLFFPLRCVGCGRRSSDLLCRTCFDALPIVGRPV
                     CERCGLPTAFETFVCEECKNVDFGFESARAPLKYAGVGKEIVHALKYRGYRSVVEKLA
                     APLMLQVLEDSRFDAVVPVPLHRARRRKRGFNQAELLARGVAEKINATVSDTLKVVRT
                     TRDQVELSAAQRRANVAGAYTARNPLSGRVLLVDDVFTTGATMSACAATLVEAGAKEV
                     HALSLCRTV"
BASE COUNT         1086 a         1619 c         1759 g          969 t
ORIGIN      
        1 gggcagggca cgcggctgag gccggtgact tacgacatcc ccaaagcgct cgtacctctg
       61 cgcaaccgtc ctttcatggg ttacatgctg gacttcctgc gcaacggcgg gctcaacggg
      121 gcggtgctgt cgttggggta cctgccggat cccatacagg cctacctgtg cggacagcag
      181 gacctcgagg gcttctccgt tgattacgcc gtcgaagacc gtgccctcgg tacggccggc
      241 ggtatcaaga acgcgcagga gtacctggac ggcgacacct tcgtcgtggt caatggtgat
      301 gtgctctgcg ggatggacct cgccaaagcg ttagagatac acagggcctc agacgccctc
      361 gcgaccatcg tcctcacgag cgtcgaggac ccgacggcgt acggcctcgt cgaggtggac
      421 cacgagatgc ttgtgcatcg ctttatcgag aagcccgcgg ccgacgaggt gacgacgaac
      481 ctggtcaacg cgggcgtcta cgtgctagag cccgagatac tcgacatgat ccccgccgga
      541 cgcgaggtct ccatcgagcg ggagatcttc cccgagctgc aggacagcgg caggctcagg
      601 gcccacatct cgagctcgta ctggcgggac atagggacgc cgcgcagcta ccttgccgcc
      661 tcacacgacg ttctctccgg cgcggtcggg gcgcgggatg gcttcgagta cctcgacgtc
      721 gacccttcgg tcgagctcgg aaaaaacgtc aggctcctgc ctcccgtctc gataggccgg
      781 gggtgcagga tctcgaacct cgccacgata ggcggacgct cctctctagg gaacggatgc
      841 gttataggag agggggccgt ggtcgagggc agcatcgtcc tcgacggcgc cgaagtggag
      901 gccggcgccg tggtgcgcgg ttctgtcatc ggcccgcgcg caagggtagg caacaacgcc
      961 atcgtgcgcg gcctctcagt gctcggcgca ggctgcgtcg tcggcgaggg taacgtcctc
     1021 gaccagggga tacgcgtaaa ccccggcgtc gttgtttcgc cacgtagctt gagcttctag
     1081 ccatcgacta gcggcgctcc ctcttgcttc gacccgtacg cagacatgcg gcctgtgatt
     1141 ggggtatcat taaggcgctc tagcaagagc atagtgaaaa tggcaaactc gtcgcgagac
     1201 ggggacgcaa agttacggac ctcgtgggag gccgccggac cgccactatg tcccgtaatt
     1261 acttgtccct tcctcggcga agaggatagg agaagtcgga tcacactagc cagcacaagg
     1321 ccttctctaa ccagtgttat cggcaagagg gggcaagact acagccatgg agcatcgtgg
     1381 ccgaggatgg gcgtaggcaa ccacagatga ccgaagccac gaggacagga agccgaaggt
     1441 gagccagatt cgcgagttcg ggccaaaggc gggccttcgc gcctggctcg tctatctggt
     1501 aatcggagtc gccgcaacca gcatattttt tctgttgcct ggcgttgccc aggcaatcct
     1561 atacaacctc atcggcgcct cgatggtgat cgcgatcctg gtcggcgtgc gcctgaaccg
     1621 ccccgagcct gcgctaccct ggtacgtcat cgccttcggc ctggcgctct tcgtgagcgc
     1681 tgacgtgatc tactacaata tctacccgaa cgtgctgggc gtccccgcgc ccttcccttc
     1741 ggtcgctgat gccttctacg ctagcagtta cctcgtcgtc gccttcgggc tggccgtact
     1801 catccgcggc gttggcggca gagaggactg gggcagcctg atcgacgccg ggatcatcgc
     1861 tgcaggtctg gggctgctcg cgtgggaatt cctgatacag aactacctgg aagccgcatc
     1921 cattccgctg ttcgtgcgcc tcgtcgccat tgactacccg ctcatggggg tggtgtgggt
     1981 agctttggca atcaggctac tgttcatgtc gcgccccccc cgcccgccgg cgctgtacct
     2041 gctcgtcctg gctgccgcgt tccatcccat aggtgatgcg atatacgcct ggctggtgct
     2101 cagggacgcg tatcaaagcg gtactgtgct cgacaccttc tggatgcttt cctatgtgac
     2161 attcggcgcg gcggctctgc acccgtccat gcgcgagctg aatgaggcta cgcgtgatcg
     2221 cgtaacgggg ctctctggct ggcgcctgac gttgctgacg gcggcggcgc tgatggtgcc
     2281 cggcgtgctc gtactgcaga cgacgctcaa cgaggctatc gacgtgcttg cgatagctgc
     2341 ggccgcgacc gtcctgttct ctctcgtact gctacgcgtg gcgggtctcc tccgcgagaa
     2401 tgagcggacg gcggcggagc tcgggcgtct caacgagagg ctcgaggagc gagtcgcgga
     2461 gcgcaccgcg gatctcgagg aagccagaga gatagccgag tcggccaaca gggcaaagag
     2521 cgagttcctt gccaacatga gccacgagat aaggacgcca atgaacggcg tcataggcat
     2581 gacggggctc atgctcggca ccgacctctc ccctgagcaa cgcgagtacg ctgagacggt
     2641 gcgctcctcg ggggagaatc tgctcacaat catcaacgac atcttggact tctccaagat
     2701 cgaagcgggc aagatggatt tggagatcat agacttcgac ctgcgctcgg cggtagagga
     2761 gtcggtgggg ctgctcgccg agcgggcaca cgacaagggc ttggagcttg cgagcctcgt
     2821 caagtacgac gtgcccacca ccctcagggg tgacccgggg cgcataaggc aagtcctggt
     2881 caacctcctc ggtaacgcgg tgaagtttac cgaaaaggga gaggttacgc taatagttgg
     2941 gctcgtcgag gacaccgaag acgcggcgat cgtgcgcttc gaggttaggg acaccggtat
     3001 agggatgacc cccgaacaga aggagcgcct gttccggtcc ttctcccagg ccgacgcctc
     3061 caccacccgc cgctacggag ggacggggct agggctcgcc atcagtaagc agcttgtgga
     3121 gctgatgggc ggggagataa gggtacaaag cgaaccggga gtgggaagct ccttcttctt
     3181 taccttgcct ttgagaaagc agcgagcggg cgcgtcatcc acacctaagt ttctcaccga
     3241 tcttaggggc cttagggtgc tcatcgttga cgacaacgag accaaccgca agatagtgca
     3301 ccaccagatc gtctcctggg ggatgaagaa cggtagagcc gaggatggac cgagcgccct
     3361 tcaaatgctg cgctctgcca aagagagcgg aaagccctac gatctggtga tcctcgacat
     3421 gcagatgccg gggatggacg gcctggagct ggcaaggaag ataaagaccg agcctctgct
     3481 ctcctctacc aagctcatca tgcttagctc catagggcgg cgcggagaag gcgaggaagc
     3541 gcgacaagcg agcatagaag cctacctcac caagcccgta aggcagtccc gactcttcga
     3601 tgctatcgcg ctcgtgatgg gtactgcggc agaggaaacc gtagcacccg aggaggagaa
     3661 gcaaaggcaa ctcgtcaccc gccacagcct caaagaggcg aaggaccgct ctcggatccg
     3721 aatcctcgtc gccgaggaca accaggttaa ccagaaggtg gcggtacaga tgctaaaaag
     3781 attgggctac caggcagacg tcgccgccga tggcctggag gtgcttgaaa ccctctcgcg
     3841 catccggtat gcggccatcc tcatggacgt gcagatgccc gagatggacg gctacgaagc
     3901 tacagccgag ataaggcgcc gcgaagaagg ccaagatcgc catacgcccg taatcgcgat
     3961 gacggccaac gccatgcagg gagatcggga gaaggcgctc gaagcgggga tggacgacta
     4021 cgtccccaag cccgtaaagc ccgaggagct gaaggcggtc cttgggcgtt ggattgctca
     4081 gcgggaggtt actctgggag cggacacgca cgcgacggca gcaggcgccg atccaacggc
     4141 atcggacgat gagcaagctc cacttgatga gagcgtgcta gaaggcctgc gcgagctgca
     4201 agcagagggc gagccggaca tcctggagga actgatagaa atgttcctgg aggaggtccc
     4261 tcctcagctg gtggccatgc gagaggcgat agaagatcgc gacgccagca ccgtcgagca
     4321 cctcgcgcac atgctgaagg gaagctcggg caacatgggg gcgctgcgga tggcccctat
     4381 ctgcggcgaa cttgaggagg tcggcgccgc ggaggaccta gtacgcgctc agaggttgct
     4441 cgaacagctc gaagtggagt tcgggcgcgt gcgcctggct ctcgaagccg agcggtcagc
     4501 gaattagtct gagatcgtga ttccagtatc ttgacgggat cgatgttcct ggtacttacc
     4561 cggattagcg ggtcgccggc cttttgagga gaatcctcga tgtacgaacc atacgcctct
     4621 gcgctggctg acctcttctt cccactacgc tgcgtcggct gcggaaggcg ctccagcgac
     4681 ctcctctgcc gaacatgctt cgatgcactt cccatagtgg gccgtcccgt ctgcgagcgt
     4741 tgcgggttgc ccaccgcctt cgagacgttc gtctgcgagg agtgcaagaa cgtggacttc
     4801 ggcttcgaga gcgcgagggc acctctcaaa tacgcgggcg tagggaagga gatcgtccac
     4861 gcactcaagt atcgcggcta cagaagtgtg gtcgagaagc tggccgcgcc gctcatgctc
     4921 caggtactcg aagatagccg cttcgacgcc gtggtgcccg taccgttgca ccgcgcgcgc
     4981 cgcaggaaga ggggcttcaa ccaggccgag ctccttgcgc ggggcgtcgc agagaagata
     5041 aacgcgaccg tatcggatac actaaaagtc gtgcgcacca cacgggacca ggtcgagctc
     5101 tcggcggccc agaggcgggc caacgtcgca ggcgcctaca cggccaggaa cccgctcagt
     5161 ggcagggtcc tgctcgtcga tgacgttttc accacaggcg cgacgatgag cgcgtgcgcc
     5221 gcaactctcg tcgaagcagg ggcgaaagag gtccacgcgc tgagcctgtg caggacggtc
     5281 taggtgtcag gcatcaggtt tcaggtttca ggtatcagca aaatacctga agcctgttgc
     5341 ctgtagcctt ttgcgaaggg aggctacatt ggacatactc atcaagggac gcaacatctc
     5401 ggtgacggag gcgctcgagc ggtacgcctg gga
//
LOCUS       DATAIR010000673        10755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_344673, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000673 DATAIR010000000
VERSION     DATAIR010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344673"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VHM69_15930"
     CDS             <1..405
                     /locus_tag="VHM69_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741933.1"
                     /translation="APIGVRRRWILRGLLTALLVLVYFGGVTATKAIFQALTGQQELP
                     QLTIVASTRVIAALFNPLRHRIQSFIDRRFYRRKYDARKTLEAFSAKLRDETDLEALN
                     NDLVGVVRETMHPKHVSLWLRSVTAAKGRQTG"
     gene            918..4184
                     /locus_tag="VHM69_15935"
     CDS             918..4184
                     /locus_tag="VHM69_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2741934.1"
                     /translation="MTGSQRPTTGQQRAPRETPGSRLGAVRIWLLDGFRISVGSSRSI
                     GEDEWRLKKAGSLLKLLALAPAHRLHREQAMDLLWPELDSEAAANNLHHALHIARRTL
                     EPSAPAGAASGYLHLRGESLALSPAGPVWVDVEAFEEAATTARHALDPAAYRAAIDLY
                     SGELLPQDRYEAWAEERRAHLRGVYLSLLWEVAGLYEERKEFGEAIEVLGRVVAEDPT
                     HEGAHVGLIRLYALLGRRREALGQYERLRDALFREFRAEPEAATVRLHQEIWAGTFPP
                     VHSPLAAGSPSEALHAPGAGGRHNLPLARTSFIGRGRETLEVKRLLAMTRLLTLTGVG
                     GSGKTRLALKVASDLAGAYPDGAWLVELAALSEGELVPQAVARALRVREQPGRPLTES
                     LKDALRARRMLLVVDNCEHLIEPVVRLVDSLLDSCPGLRVLATSRETLSAAGEVAWVV
                     PPLTVPDSGRASTAEKLEGYESVRLFVERARQRDPSFVLTPRNGPPVAQVCRRLEGIP
                     LAIELAAARIGVLSVERIAERLDDSLRLLTAGGRTAQPRHRTLRATLEWSHELLGEPE
                     RKLFRRLSVFAGGLTLEAAEAVGAGDGIEHHDVLDLLSKLVDKSLVLAEASPAEEGAL
                     RYRMLEPLRQYGQEHLEESGEAQRVRERHAEYYLALAEGADAQEAERELNAARPVAWL
                     ERMESEHANLRAALNWSLDQYDEPDGGRAEQLGLRLAVALWWFWHTHDYLSEGRRYLQ
                     RALSGRSDPAMIRWRARALDAAAGLALYQADYGASKALMEEALALYRELGDKEGIAAG
                     LTDIGLVAVLGQRDDIPLPAVLEELGELKPELKNRNTLAYLLMLEGLIALSRGDPEPS
                     VAQHEQSLDLFREIGNTQGIITCLGHLGLLALVRGDYETAPTLLRESLRLAWELDYKQ
                     SIQHCLYTLACVTACREQPVRATRLWGAVEGMEEAYGAHLTPVILSLTNYEGHLSRGQ
                     SLLGEEGFAVAWAGGKAMSLGQAIEFALSDKEERDPPTLVPVTEQQPDERTDRLTRRE
                     REVALLVARGLTNRQIASELSVSRSTANNHVARILRKLGLRSRAQIAAWVTERRSPSS
                     "
     gene            4384..5742
                     /locus_tag="VHM69_15940"
     CDS             4384..5742
                     /locus_tag="VHM69_15940"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX2741935.1"
                     /translation="MSTKEGDGYDVSSGTISRRGALKLLGGAGLGLLVLPVFPSSAYA
                     AGKLQPVLKTSPLGKFGGVQYVQHDGLFVGTTSTGNYSVPYRISAPANPRRAIRSTVL
                     VEPPHFATGTYLRDGWLGRPFLFGRGFLHASVGYSTTTSGPFVFRILDPTAQGAFIDG
                     GVVDGNGRTDDEIVVDFARALRSDPLARALMGPLGRRYLAGFSDSADPVKRIVSSGLA
                     EDEFDLAVPITTGSEGDPQESIAAGKYSGKVIAVNSEAEWFDGRALEDRGTTPDHYRF
                     FIVPGTPHVPDPLCPGFFSNETTPAGWQPALRAHFLQGHAWVTRGDAPPASTRLATAN
                     GDAEIARDPNGNALLVEITGVSAPRLPFVELGEARFITGFLGTYEPQPPPTIEQLGFS
                     SFPEYLAAFEEALKAQVQARYMLREDAKVQLMRAGLSSPATFTENYFRRYDEFRSGTY
                     CP"
     gene            5870..6502
                     /locus_tag="VHM69_15945"
     CDS             5870..6502
                     /locus_tag="VHM69_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741936.1"
                     /translation="MASKVAWMARVTTTVVGLAIMLAMMFGAASAAFGANGGNFILGQ
                     LNKATAMTRLTANVAGNPALQLTNNNPAAGSRALHLNAFSGRAPLTVNETAGRATNLN
                     ADKVDGSEGEMWAQINENGTADNWKGMTGNSRFAEGVYIISFARDDVRGCAISTSIAS
                     HGAPHFPGSITATTTDSVPNTVTIFSYDSSGVAEDRDFHLVLFCGDHQSA"
     gene            complement(6588..6866)
                     /locus_tag="VHM69_15950"
     CDS             complement(6588..6866)
                     /locus_tag="VHM69_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741937.1"
                     /translation="MIASAANDATRLLRAINMYQAHGRIGATIFAVDAARVAGIDPAT
                     KRYEEAVWYLLWNGALTVNECVPPEIAARVPFGRAPYRLAPAAIRMLE"
     gene            7374..8534
                     /locus_tag="VHM69_15955"
     CDS             7374..8534
                     /locus_tag="VHM69_15955"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX2741938.1"
                     /translation="MDKEFRVELAAVYDLACEKIARTAGRLEGSEEFPHDTDDGRWRT
                     WPADVYAGWEGDSWSHGNWTCGFWVGMLWLALSRTHDPRFEEWARYFGDLVAPRQRDE
                     NTHDIGFIFYPSFALGHRVTGDERLREPALQAARTLATRFNPRGGYLQAWGPLDHPRA
                     MGSTAIDTMMNLPLLWWASRITEEPEFAEIARAHAATSARYYLRPDGSTYHIYTFDPD
                     TGTGIEGGTYQGANPDSTWSRGVTWGIYGWALAYREHRDPAFLEAAERAADYFVGALP
                     ADLVPPWDFADEDPSSPKDSSATAICANAFLELGEVHPDPLRRAYYRSLADAMLASLC
                     RDYIGRDEEEGILLHSAYSVPHNDGVDSSVMWGEWFFLRAVSHLTAGPIPIP"
     gene            8566..9360
                     /locus_tag="VHM69_15960"
     CDS             8566..9360
                     /locus_tag="VHM69_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2741939.1"
                     /translation="MCAENLVLVERSEGGVAKLTLNNPPLNLVTLEMTQRLIEALEDL
                     DGDESVRAVVVTGAGDEAFCAGADVKEFADVRDRVVEKKLARENRAFSAIETLSRPVI
                     AAVEGLAYGGGCEISMACDLRIIAAGARMALPEVKLGVVPGAGGLFRLPELVGPARAM
                     ELMYLGDPIDAREAEIIGLVNEVVPDGEALPRAIDIARKISRQPKEAVAAIKRGVRES
                     AHLSREEAVQLTLELSDRVFKTGDCDEGIRAFFEKREPRFEGAPVD"
     gene            9391..10596
                     /locus_tag="VHM69_15965"
     CDS             9391..10596
                     /locus_tag="VHM69_15965"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2741940.1"
                     /translation="MNYTERTGMLNGVKVLSFTHFLQGPSAVQILADLGADVIKIEPP
                     KGAWERHWSGAEGFLNGVSVFFLLGGRNQRSLGVDLRSEEGKEIIWRLIRESDVVVEN
                     YRPGAMERLGFGYERVSQEKPDIVYCSLSGYGTDGPYKERAGQDLLSQAMSGLAMING
                     RKSDPPTLVGSAVVDQHAAVLGALGVLAALYERRDTGMGKRVDSNLLNAALDLQIEPF
                     IYHLNGSLYERSDTGISSRFHQSPYGVFETADNFICVSLTPPEKLAEAFGDDTFLEWT
                     PEDQFRKREEVNAHVAGHIRRKPAEHWYKVFDEVGIWYSPVNSYEEVEKDPQVLHNEA
                     IMTFDHPTAGTVRVLSHPVRYDGAPPPLRAVPPELGEHTEEILAELGYDEESITRLKA
                     EGAVRVKDR"
BASE COUNT         2039 a         3266 c         3527 g         1923 t
ORIGIN      
        1 gcacctatag gtgtgcgtag aaggtggatt ctccgaggtt tgctcaccgc cttgctagtg
       61 ctggtttact ttgggggtgt aacggctacc aaggcgattt tccaggcgct caccggccaa
      121 caagagcttc cgcagctcac catcgttgct tccacccgcg tgatagccgc gctgttcaac
      181 cctctaaggc atcgcattca gtcgttcata gataggcgct tctaccgccg caagtacgac
      241 gccagaaaga ctctcgaagc cttctctgca aagctcagag atgagacgga tctggaggcg
      301 ctcaataatg atctggttgg agtggtgagg gagacgatgc acccgaagca cgtctctttg
      361 tggttgcgct ctgtcacggc tgctaagggc cggcagacgg gctagcgacc cgagtgcgtg
      421 gaagttaaca gcgtgttgaa aagcgtcttg ctggaccccg cgagagaagc ctctcgccat
      481 gcccccgaag ttcacggaac ccaaagcgtc cggcttcggg tcgtcggaga gcggccacct
      541 gcgccactct cctcgcggtt ccgaaggcca ggagccgctt gacgttctgc cccgaagcga
      601 tctgcacagc ttctatgttc acattctcga gcttccccgg tcggaaccct ctcatcccgt
      661 gccattcttt agcctcggcg aacagaggct ctgaccaaac cctccgcttt cgtagggctt
      721 tctcgtaggc gaaagattcg cggtaggtgc tcactctgtc gtggtagtcc tcgtagaacg
      781 atcggtacgg tacaacttgc gaatggcctc gacaccgtcg ggagggagga tctctcggct
      841 atagcagtct cgcttgcgct ttgtgtgatc ccgcgctatt aattgcacta cctcgtattt
      901 cgtggggaag cgagaggatg acgggctctc agcgaccgac aaccgggcag cagcgcgccc
      961 cgagagagac gccgggtagt aggctcgggg ctgtacgtat ctggctgctg gacggcttcc
     1021 ggatatcggt tgggtcttcc cggagtatcg gggaggacga gtggcgcctc aagaaagccg
     1081 gcagccttct caagctgctc gccctggcac cagctcaccg ccttcaccgc gaacaggcga
     1141 tggacctgct ctggccggag ctagactcgg aggccgcggc caacaacctg caccacgcgc
     1201 tgcacatcgc ccgccgcacc ctcgaaccct cggccccggc aggcgccgct tccggctacc
     1261 tgcaccttag gggcgagagt ctggccttgt ccccggcggg acccgtgtgg gtcgacgtcg
     1321 aagccttcga ggaagcggcg accacggctc gccacgccct cgatcccgcg gcctaccggg
     1381 cggccattga tctgtactcg ggggagctct tgccgcagga ccgctacgag gcctgggcgg
     1441 aggagaggcg ggcacatcta cgaggggtgt acctctcgct gctttgggag gtggccggat
     1501 tatacgagga gcgaaaggag ttcggggaag caatcgaagt gttggggagg gtggttgccg
     1561 aagatccgac gcacgaggga gcgcacgtgg ggctcatacg actgtatgcc ctcttgggga
     1621 ggcgaagaga ggccctgggg cagtacgagc ggctcaggga tgccctcttt agggagttta
     1681 gggccgagcc cgaggcggcg acagtacgcc tgcaccagga gatctgggct ggcaccttcc
     1741 caccggtcca ttcgccgctg gccgcaggct ccccatcgga agcgctccat gcgccgggtg
     1801 caggtggcag gcacaacctg cctctggcgc gcaccagctt tattgggcgc gggcgggaga
     1861 cgctcgaggt caagcggctt ctggccatga cccggctgtt gacgctcacg ggggtgggcg
     1921 gctcgggcaa gacgcgattg gcgctcaagg tggcatcgga cctggcaggg gcctacccgg
     1981 atggggcatg gctggtggag ctggcggcgc tctcggaggg agagctggtg ccccaggcgg
     2041 tggccagggc tcttagggtg cgcgagcagc ccgggcgccc gcttaccgag tcactcaagg
     2101 acgccttgag agccaggagg atgctcctgg tggtagacaa ctgcgagcac ctcatagagc
     2161 cagtagtccg cctggtggat tccctcctcg attcctgccc gggcttgcgg gttctcgcga
     2221 ccagcaggga gacgctcagc gcagcgggcg aggttgcctg ggtggtgccg ccgcttacgg
     2281 tgcccgactc cgggcgggcc tcaacggccg agaagctcga gggttacgag tcggtcaggc
     2341 tgttcgtaga acgggcccga cagcgagatc cctccttcgt cttgaccccc cggaacgggc
     2401 cgcccgtggc tcaggtatgc cggcggttgg agggcatccc tctggccata gagctggccg
     2461 ccgcgcggat aggggtgctt tcggtggagc ggatcgccga aagactcgac gactccttga
     2521 gactccttac cgcaggcggg cggacggccc aaccccgcca tcggacgctc agggcgacgc
     2581 tggaatggag ccacgagttg ctcggcgagc ccgagcggaa gctgttcagg cggctttcgg
     2641 tattcgccgg gggcttgacg ttggaggccg cggaggcggt gggggcgggt gacggcatcg
     2701 agcaccacga cgtcctggac ctgttgtcta agctggtgga caagtcgctg gtgctggccg
     2761 aggcttcgcc tgcggaggaa ggggctttgc gctacaggat gctggagccc ctcagacagt
     2821 acggccagga acatctggag gagagcgggg aggcgcagcg ggtcagggag cgccacgcgg
     2881 aatactacct ggcgcttgcg gaaggagcag acgcgcagga agcagagcgc gaattgaacg
     2941 cagcgcggcc ggtggcgtgg ctcgagcgga tggagagcga acacgccaac ctcagggcgg
     3001 ctctcaattg gtcgctagat cagtacgacg agcccgacgg cggacgtgct gagcaactgg
     3061 ggttacggct ggcggtcgcg ctgtggtggt tctggcacac ccacgactac ctgagcgagg
     3121 ggcgaaggta cctgcagaga gccctctccg ggaggagcga ccctgccatg atccgttgga
     3181 gagcgcgggc gttagacgcg gccgccgggt tagcgctcta tcaggcggat tacggggcgt
     3241 cgaaggccct gatggaggag gcgcttgccc tctaccggga gctaggagac aaggagggca
     3301 tagcagccgg cctcaccgac atcggtttgg ttgcggtgct tggccagcgt gatgacatcc
     3361 ccctgccggc agtgctggag gagctcgggg agctcaagcc ggagctaaag aaccggaata
     3421 cgctcgccta cctgctcatg ctcgagggtc tcatagcgct gagcaggggt gatccggaac
     3481 cttcggtagc gcagcacgag cagagcctcg atctgttccg cgagataggg aatactcagg
     3541 gcatcatcac gtgccttggc caccttggcc tcctggcgtt ggtccggggc gactacgaga
     3601 ctgccccgac actgctgcgg gaaagcctgc gccttgcgtg ggagttggat tataaacaga
     3661 gcatccaaca ttgcctctac acgctggcgt gcgtgaccgc ttgccgcgag cagccggtcc
     3721 gagccaccag gctgtggggg gccgtggaag gaatggagga ggcttacggc gcacacctca
     3781 cccccgtaat tctctctctc accaactacg agggccacct gagcaggggg caatcgttgc
     3841 tgggggagga ggggttcgcc gtggcgtggg cggggggcaa ggcaatgtcc ctggggcagg
     3901 ccatagaatt cgccctctcg gataaagagg agcgggaccc tcccacgctc gtccccgtga
     3961 ccgaacagca gcccgacgaa cgaacggatc ggctcacccg ccgcgagcga gaagtcgcgc
     4021 tcctcgtcgc gcgggggctc accaaccgcc agatcgcctc ggagctttcg gtctccagga
     4081 gcacggccaa caaccacgtg gccaggatct tgcgcaagct ggggctccga tcccgtgccc
     4141 agatcgccgc ctgggtaacc gaacgacgtt ctccctcctc gtagcggaga accctttccc
     4201 gcccgagggg cgttacaggg cgagccattc ttttctcgat ctaaccaaga aatagttaag
     4261 tgtaactacg cccttgggtc ctccagctaa gagtcagcta agaaccacgc cctatagtgc
     4321 tcacgcgtga gtggacaatc gtcataagta gggtcgacaa agagcagaac gaaggctacg
     4381 aaaatgagca caaaagaagg tgacgggtac gatgtctcta gtggcaccat ctctcggcgc
     4441 ggggcgttga agctgttggg tggcgcaggc cttggcctgc tcgttttgcc cgtatttccg
     4501 tcgagcgctt acgccgcagg gaagctccag ccggtgctca agacctcccc gctcggcaag
     4561 ttcggcggag tccagtacgt tcagcacgac ggattattcg tcggcacgac gtccacgggg
     4621 aactacagtg tgccgtaccg gatcagcgcg ccggccaacc cgaggcgcgc tatccgcagc
     4681 acggtgctcg tcgagccgcc gcacttcgca acgggcactt acctgcgtga tggctggctc
     4741 ggccggccgt tcctgttcgg acgaggattc ctgcatgcgt cggtcggcta tagcacgact
     4801 acctccgggc catttgtctt ccggattctg gatcccacag cgcagggcgc cttcatcgat
     4861 gggggagtcg tcgatgggaa tggccggacc gacgacgaga tagttgtcga cttcgctcgc
     4921 gcgctgagga gcgatccgct cgctcgcgcc ctcatgggac cgcttggccg gcgctacctc
     4981 gcggggttct ccgattccgc ggacccggtg aagaggatcg tcagctcggg gctcgccgag
     5041 gacgaattcg atctggcggt gccgatcacc accggcagcg agggcgaccc ccaggagtcc
     5101 atcgcagccg gcaaatacag cggcaaggtc atcgccgtca attccgaggc cgagtggttc
     5161 gacggccggg ctctcgaaga tcgcggcacg acccccgatc actatcgctt cttcatcgtg
     5221 ccgggcacac cgcacgtgcc ggatccgctg tgtcccggct tcttctccaa cgagacgacg
     5281 cccgccggct ggcagcctgc gctccgcgca cacttcctgc aaggccacgc ctgggtcacg
     5341 cgaggggacg cgccgcccgc gagcacacgc ctggccacgg ccaatggcga cgccgagatc
     5401 gcccgcgacc ccaatgggaa cgcgctgctg gtcgagatca ccggcgtctc cgcgccgcgg
     5461 ctgcccttcg tcgagctcgg cgaggccagg ttcattaccg ggtttctggg cacctatgag
     5521 ccacagcctc cgccgacgat cgagcaactc ggtttctcca gcttccccga atacctggcc
     5581 gcgttcgagg aagcgctcaa ggcacaggtg caagcccgct acatgctcag ggaggacgcg
     5641 aaagtccagc tgatgcgcgc aggtctttcc tcaccggcga cgtttacgga gaactacttc
     5701 agacgctatg acgagttccg atccggcacg tactgtccat gacgctccgc tcgtcctacg
     5761 ttggagatct gcctatgctc caccgcaggt gggtacaccc aaccgcgctg aagttcatga
     5821 tgcttttgtt gctacaagga cttgaggagg catggctatg ttgaggagca tggcgagcaa
     5881 ggtggcgtgg atggcgaggg tcaccacgac cgtcgtgggc ctggcgatca tgctagcgat
     5941 gatgttcggg gcggcttctg cggcattcgg ggcaaacgga ggcaacttca tcctggggca
     6001 gctcaacaaa gctaccgcta tgaccaggtt aacggccaac gtcgcgggca accctgcttt
     6061 gcagttgacg aacaacaatc ccgctgcggg ttccagagct ctgcatctca acgcgttttc
     6121 gggtcgtgca cccctgacgg taaatgagac agcaggcagg gccacgaacc tcaacgccga
     6181 taaggtggac ggaagcgagg gggagatgtg ggcccagatc aacgagaatg gcaccgccga
     6241 taactggaaa ggcatgaccg gcaactccag gtttgcagag ggcgtctaca tcatcagctt
     6301 cgcacgcgac gacgtgcgcg gatgcgcgat ctcgacctcc atcgcaagcc acggtgcgcc
     6361 gcattttcca ggttccatta cggcgacgac gacagacagt gttcccaaca ccgtaaccat
     6421 atttagctac gactcctctg gagtggcgga agatagggac tttcatctag tgttgttctg
     6481 tggcgatcac caaagtgcct agagaggttc atgttcgaga ccttgcgagt cgtctacttt
     6541 caacaggccg accccctcca ggaaggtggg tgaataagga ctcacctcta ctccagcatg
     6601 cgtatggccg ccggagccaa cctgtacggt gcccggccaa acggtactct ggccgcgatc
     6661 tccggaggga cgcactcgtt taccgtgagc gccccattcc acagcaggta ccaaaccgct
     6721 tcctcgtagc gcttcgtggc agggtcgata ccggcgacgc gggcggcatc gaccgcgaag
     6781 atggttgccc cgatgcgccc gtgggcctga tacatgttga tagcgcgcag caacctcgtg
     6841 gcatcgttgg cggcactcgc aatcatgcag ctcctctccg caagcggggc gaatagggta
     6901 tgcgcaccgc cgctctttgc aggctacggg tcggggaagg gcggcgcatc tgccacatgg
     6961 cgtatttttg gtcgctggtg tgttaccagc ggaggcgatt ctccgaaaac actcgcaagg
     7021 tgaggtgaac tagcgtccgc ctcgctctcg ggtggaatag tcttccgatg tcggctgctt
     7081 ccctcatcgg ggcgtccggt gcaacttaca aggagcgtga cgccagggat cctcggccgc
     7141 tcgaacttgc tttcggggtc gctgctgctg ggttcgcgcg cgaaccacgc tcacgaggag
     7201 cgtcttgaaa gtcctctggt gaacagatag ggtttctcac aatcggggag ggaaggagag
     7261 tggagtaatg agacctgaac cagttttaga tgatctcatt ctcgccaata tgctgacggc
     7321 cacctgggtt gggattccgg gctggcatcc acatttgggc gcgaggcggt agcatggaca
     7381 aagagttccg agtcgagcta gccgcggtct acgacctggc gtgcgagaag atcgcacgca
     7441 ccgccggccg tctcgaaggg agcgaggagt ttcctcacga taccgacgat ggccgctggc
     7501 gcacgtggcc ggccgacgtg tacgcgggat gggaaggcga tagctggagc cacgggaact
     7561 ggacgtgcgg gttctgggtt ggcatgttgt ggctcgctct ctcacggacc cacgacccac
     7621 gtttcgagga gtgggcaagg tattttggtg acctggtggc gccccgacag cgcgacgaaa
     7681 acacacacga catcggcttc atcttctatc cgagcttcgc gcttggccac cgggtcacgg
     7741 gggatgagag actcagagaa cccgcgttgc aagcggcgcg cactctggcc acccgcttca
     7801 acccacgggg tggctatctc caggcctggg ggcccctgga ccacccacgg gccatgggat
     7861 ccacggctat cgatacgatg atgaacctgc cgctcctctg gtgggcttcc cgtataacgg
     7921 aggagccgga attcgccgag atagcccgcg cacacgccgc cacctcggcc cgctactacc
     7981 tgcggcccga cggttccacg taccacatat acaccttcga ccccgacacg ggcaccggca
     8041 tcgaaggcgg cacctatcag ggggctaacc ccgactcgac gtggagccgg ggagtgacgt
     8101 gggggatcta cggttgggcc cttgcttacc gcgagcaccg cgaccccgct tttctggagg
     8161 cggcggagag ggcggcggat tacttcgtcg gagccctgcc cgccgacctg gtcccgccct
     8221 gggacttcgc cgacgaagat ccttcgtccc cgaaggattc ttcagccacc gccatttgcg
     8281 ccaacgcttt cctcgaactg ggggaggtgc acccggaccc actgcgtcgc gcctactacc
     8341 gtagccttgc cgacgccatg cttgcctccc tctgcaggga ttacatcggg cgggacgagg
     8401 aagaaggcat actactgcac tccgcctact cggtgccgca caacgacggg gtagatagct
     8461 ccgtgatgtg gggcgagtgg ttcttcctca gggcggtgag ccacctgacg gcaggcccga
     8521 taccgatccc ctgaatgaaa tggacgctca tgaaggcgag gatgcatgtg tgctgaaaac
     8581 ctggtgctcg tggagcgctc ggaagggggt gtcgcgaagc tcaccctgaa caatccgccg
     8641 ctcaaccttg tcacgctgga aatgacgcag cgcctgatcg aagccctcga agacctcgat
     8701 ggagacgagt ccgtccgcgc ggtcgtcgtt accggcgcgg gtgacgaggc tttctgcgcc
     8761 ggggccgacg tgaaagagtt cgcggacgtt cgagaccggg tcgtagagaa gaaactcgcg
     8821 agagagaacc gggcattcag cgccatcgag acgttgtcca ggccggtgat cgctgccgtc
     8881 gagggcctcg cctacggcgg aggatgcgag atctccatgg cctgcgacct gaggataatc
     8941 gctgctggcg cgaggatggc gctccctgag gtgaagctcg gcgtcgtgcc tggggccgga
     9001 ggtcttttcc gcctgcccga gcttgtgggt cctgcacggg ctatggagct aatgtatctc
     9061 ggcgatccca tcgacgcccg cgaggctgag atcattggcc tggtcaacga ggtcgtcccg
     9121 gacggggagg cgctcccacg cgccatcgac atagcgcgga agatctcccg ccagccaaag
     9181 gaagccgtcg cggcgatcaa gcgcggggtg agggagtcgg cgcatctctc ccgcgaggaa
     9241 gccgtccaac tgaccctcga gttgagcgac cgcgtcttca agaccgggga ttgcgacgag
     9301 gggatcaggg ccttcttcga gaagcgggaa ccacgcttcg agggagcccc tgtagactaa
     9361 cggcgaaact cagaggcggc gctctgtgtc atgaactaca cggaaaggac tggcatgttg
     9421 aatggtgtaa aggtcctgag cttcacgcac ttcctgcagg ggccttcagc ggtccagatc
     9481 ctggctgacc tcggggctga cgttatcaag atagaaccac cgaagggggc gtgggagcgc
     9541 cactggtcgg gcgcggaagg gttcctgaac ggggtgagcg tcttctttct cctcggcggg
     9601 cgcaaccagc gcagtctggg agtggatctg cgctctgaag agggaaagga gatcatctgg
     9661 cgtctgatcc gcgagtccga cgtcgtggtt gagaactacc ggcccggcgc catggagcgt
     9721 ctgggattcg ggtacgaacg ggtctcccag gagaagccgg acatcgtgta ttgttcgctc
     9781 tccggttacg gaacggacgg gccatacaaa gagcgggcgg gccaggacct cctcagccag
     9841 gccatgagcg gtctcgcgat gatcaacggt cgcaagagcg acccgcctac tctggttggg
     9901 tccgccgtgg tggaccagca cgccgccgtg ctcggggctc ttggtgtact ggccgcgctc
     9961 tacgaacgca gggacacagg catgggaaag agggtggaca gcaacctgct caacgccgcg
    10021 ctcgacctgc agatcgaacc ctttatctac catctcaacg gtagtctgta cgaacgtagc
    10081 gacacaggca tctcctcacg gttccaccag tcgccctacg gtgtcttcga gaccgctgac
    10141 aacttcatct gtgtttcgct gacgccgccc gagaagctgg ccgaggcgtt cggggacgat
    10201 acgttcctcg agtggacacc ggaagaccag ttccgaaaaa gggaagaagt gaatgcccac
    10261 gtggccggcc acatcagacg taagccggcg gagcactggt acaaggtgtt cgacgaagtc
    10321 ggaatctggt actcgcccgt caactcctac gaggaggtgg agaaggatcc tcaggtcttg
    10381 cacaatgagg cgataatgac cttcgatcat cccaccgccg gaacggtccg cgtactgtcc
    10441 catccggtgc gatacgatgg ggcaccacca ccgctgcgcg cggtgccccc cgaacttggc
    10501 gagcacaccg aggagatact cgccgaactg ggctacgacg aggaatccat aaccaggcta
    10561 aaagcggaag gagccgtacg tgtcaaggat cgatgatcag aaccaggaaa tgacggcgag
    10621 caaggtcatc ccgctcgaag ccggcgattc agtctcgttc agcaagacgg ttggcgagtc
    10681 cgacgtctat ttattcgcag gcatcacggg cgatttcagc cccaaccaca tcaacgaaga
    10741 gatcatgagc aagac
//
LOCUS       DATAIR010000674         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_160441, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000674 DATAIR010000000
VERSION     DATAIR010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160441"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2922)
                     /gene="cobN"
                     /locus_tag="VHM69_15970"
     CDS             complement(<1..2922)
                     /gene="cobN"
                     /locus_tag="VHM69_15970"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX2741941.1"
                     /translation="MSATPEREAIRSKVRGSGSGEILFLTTADTEILAAARASERLPE
                     GFPRLRCANPVTLDDAGSFLELDETRVVLVRLLGGRRAWPEGLEELRLRCAQMRVPLL
                     AFGGEAEPDAELAALSTVPSGTVLDAFEYLRHGGVSNTENLLRFVADTVLVEGYGFEP
                     ASPLPEVGVYHPRLPEGSPVEDLLSTHDPARPTVGVIFYRAHWMGGNTAFVDTLVGAL
                     EEAGTNALPVYCYSLRAGADGAVPALEILNNRVDCLITTVLAGGGSNAADAQRSGTPE
                     EWLEWEVPALEELGVPVLQGICTTSTREAWLASDAGLSPLDTAWQVAIPEFDGRIIGV
                     PFSFKERLGEGSPVGAPLTLYSADPERTGRLAGLAARFASLGRVPNPEKRISILLSNY
                     PTKHSRVGNAVGLDTPASAIGLLGAMREAGYRVEDAPDDGDELIHSLIAAGGHDLEFL
                     TGDQLKGAAGRLDAKRYAEWFGRLPEGLRSEVEEHWGPPPGELYVDRGDFVVAGLTFG
                     NVFVGIQPPRGFGENPIAIYHDHDLPPTHHYLAAYWWLIEEFGADAVIHLGKHGTLEW
                     LPGKSLGLSPSCAPDAVLKDVPLFYPFVVNDPGEGTQAKRRAHATVVDHLIPPMTRAE
                     TYDDLAKLEQLLDEYYQVETLDPSKLPAIRVRIWETLREAELHRDLGVEEQPEEFSDF
                     LTHVDGYLCEIKDLPIRGGLHVLGETPEGEPLRHLVASILRLGAGEVPGLRRAVGTAY
                     GLDERSLAENGGLRAEAPENLAQRFPAVVATASDLIDRLEEATQALLGEMDTRGWDAD
                     AAGAVCEEVLGVADAGVERSLRFAADEVVPRLMRTPEEMENLLSGLRGGYVPAGPSGS
                     PTRGLVNVLPTGRNFYSVDPKALPSRLSWEVGQGLADDLLRRYIEEEGRYPESVGIVV
                     WGTAAMRTQGDDIAEILALLGVRPVWNEESRRVTGLEVVPLEELERPRIDVTVRISGF
                     FR"
     gene            complement(2919..>4227)
                     /locus_tag="VHM69_15975"
     CDS             complement(2919..>4227)
                     /locus_tag="VHM69_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2741942.1"
                     /translation="PGTKATVDDLKLLRTRGLDRALAERADLGLPMLGICGGYQMLGE
                     RIVDGVESGEPEIMGLGLLPVETFFEEDKVLGRPGGRASRFGDAAVSGYEIHHGRVRR
                     YGGEPLFDAGDETEGCLTGATLGTSWHGVFESDGFRRALLSWVARERGLDWRPGEESF
                     AAVREAQLEKLGDLVADHVDREALLSLVEGGAPRGLPVVVGTLEEQPEIPLAGGRVTE
                     GVVRVGGTVRRPVGSHTHLAHALLSRLEAVGFDGAPRVLGIDERGREILTFIEGEVPP
                     DLGRFSDRQLVEAATLIRRFHDATADSELAGVEEVVCHNDLSPCNAVFVNGTPRALID
                     FDASAPGTRAMDLGYALWMWLDLGHEGPDAREQGRRMRLMLDAYGLDGDPTEMVLNSV
                     RATIRRTRGRREVVQWMESVAAWVKNNRDILTTYAKPREVPRT"
BASE COUNT          710 a         1464 c         1339 g          714 t
ORIGIN      
        1 cctgaagaag ccgcttatcc tgacggtaac gtcgatcctc ggcctctcca gctcttcgag
       61 tggaaccacc tcaagcccgg taacccgacg agattcctcg ttccagactg gcctcacgcc
      121 gaggagggcg agtatctccg ctatgtcgtc gccctgtgtt ctcatggccg ccgtgcccca
      181 gaccacgatc ccgacgctct cgggataccg accttcctcc tctatgtagc gccgcaacag
      241 gtcgtcagcg agcccctgcc ccacctccca agagagcctg gacggcagag ccttcgggtc
      301 cacggagtaa aaattgcgtc ccgttggcag tacgttcacg agcccgcgag tcggagagcc
      361 agacggtccg gcaggcacgt acccgcccct gaggccggag agaaggttct ccatctcctc
      421 gggtgtccgc atcagacgcg gcacgacttc gtcggcggcg aaccgcaaag aacgctccac
      481 cccggcgtca gcgacgccaa ggacttcctc gcagactgcg cccgcggcat cggcgtccca
      541 gccacgggta tccatctccc ccagcagggc ctgggttgcc tcctcaagac ggtcgatgag
      601 gtccgaggcg gtggctacga cggcggggaa gcgttgcgcc aggttctcag gtgcttcggc
      661 gcgcaggccg ccgttctcgg cgagggacct ctcgtcgagg ccgtaggcgg tgccgacggc
      721 gcgacgcagg ccgggcacct cgcccgcacc gaggcgcagg atggaagcga cgaggtggcg
      781 caggggctcg ccctctggtg tctcgccgag gacgtggagg ccgcccctta tggggaggtc
      841 tttaatctca cagaggtatc cgtccacgtg ggtgaggaag tcgctgaact cctccggctg
      901 ctcctcgacg ccgaggtcgc ggtgaagctc cgcctcgcgc agggtctccc agatacgaac
      961 ccggatcgcc ggcagcttcg atgggtcgag cgtctctacc tgatagtact cgtccaggag
     1021 ttgctccagc ttcgccaggt cgtcgtaggt ctcggcgcgg gtcatcgggg ggatcagatg
     1081 gtccaccacc gtggcatgcg ccctcctttt cgcctgcgtc ccctcgccag ggtcgttgac
     1141 gacgaaggga tagaagagcg gcacgtcctt gaggacggcg tcaggggcgc acgatggcga
     1201 gagtccgaga gactttccag gcagccattc cagggtgccg tgcttgccga ggtggatcac
     1261 ggcgtcagcc ccgaactcct cgatgagcca ccagtaggca gcgaggtagt ggtgcgtcgg
     1321 ggggaggtcg tggtcgtggt agatcgcgat cgggttctcc ccaaatcccc gcggcggctg
     1381 tatcccgacg aagacgttcc cgaaagtgag ccccgccacc acgaagtctc ccctatccac
     1441 gtaaagctct ccgggcggtg gtccccagtg ctcctcgacc tcgctacgca agccctcggg
     1501 cagcctgccg aaccattcgg cgtagcgttt cgcatccagc cgtcccgcag cgcccttgag
     1561 ttgatctcct gtcaggaact ccaggtcgtg cccgcccgcg gcgatgagcg agtggatgag
     1621 ctcgtcccca tcgtcaggcg catcttccac tctgtaaccg gcctcccgca tcgcaccgag
     1681 cagcccgatg gcgctcgccg gggtatccag cccgaccgcg ttgccgaccc gcgagtgctt
     1741 ggtcgggtag ttggagagca agatggagat cctcttctcc gggttcggga cgcgcccgag
     1801 gctggcgaag cgcgccgcga gccctgcgag gcggccggtg cgttcggggt cggcgctgta
     1861 gagggtgagt ggagcgccga ccggggagcc ttcaccgagg cgttccttaa aagagaaagg
     1921 gacgccgatg atacggccgt cgaactcggg gatcgccacc tgccacgctg tatccagcgg
     1981 ggagagaccg gcgtcggagg cgagccaggc ttcgcgcgtc gaggtcgtgc agatgccctg
     2041 taacacgggt acgccaagct cttcgagagc agggacctcc cattcgagcc actcctctgg
     2101 agtaccggat ctctgcgcgt ccgcggcgtt ggaccccccg cccgcgagca cggtagttat
     2161 gagacaatcg actctgttgt tcagtatctc cagggccggc actgctccgt cggcgcctgc
     2221 gcgcaacgag tagcagtaga cgggcagggc gttggtcccg gcctcctcca gcgctccgac
     2281 aagcgtgtcg acgaaagcgg tgttcccgcc catccagtgg gcgcggtaga agatcacacc
     2341 caccgtaggc cgcgccgggt cgtgcgtgga gaggaggtct tcaacaggag aaccctcggg
     2401 caaacgtgga tgatagacgc cgacttcggg cagcggcgac gcgggctcga acccgtagcc
     2461 ttccacgaga acggtgtcgg cgacgaatcg gagaaggttc tccgtgttgc tgacgccgcc
     2521 gtggcgcagg tactcgaacg cgtcgagaac tgtccccgaa gggacggtcg agagcgcggc
     2581 gagctcagcg tcgggctcgg cctccccgcc gaaggcgagc agcgggactc tcatctgcgc
     2641 gcagcgcaac ctcaactcct caagcccctc tggccacgcc ctccgcccgc cgagaagccg
     2701 cacgagcacg acccgcgttt catccagctc caagaacgac ccggcatcat cgagcgtgac
     2761 ggggttggcg cacctgagcc tcgggaagcc ttctggcaga cgctcagagg cccttgcggc
     2821 ggccaggatc tcggtgtcgg cggtggtcag gaacaggatc tcgccggacc ccgaacccct
     2881 gacctttgat ctaatagcct ctcgctctgg tgtggcgctc aagtccgcgg cacctctcgt
     2941 ggcttcgcat acgtcgtcag gatgtccctg ttgttcttca cccaggccgc cacgctctcc
     3001 atccactgga ctacctctct gcgtcccctg gtccggcgta tcgtcgcccg cacgctattc
     3061 aagaccatct cggtcggatc cccatcgagg ccgtaggcgt cgagcatgag tcgcatccgc
     3121 ctcccctgct cgcgggcgtc tggaccttcg tgcccgaggt ccagccacat ccacagcgcg
     3181 tacccgaggt ccattgctct cgtacccggt gcgctcgcgt cgaagtcgat gagcgcccgc
     3241 ggagtcccgt tcacgaagac cgcgttgcag ggtgagaggt cgttgtggca gacgacctcc
     3301 tcgactcccg ccagttccga gtccgccgtg gcgtcgtgga agcgccggat caacgtcgcc
     3361 gcttcgacga gttgccggtc ggagaagcgg cccaggtcgg gcggcacttc gccctcgatg
     3421 aaggtgagga tctctcgccc gcgctcgtcg atcccgagca cccgcggggc tccatcgaaa
     3481 ccgacggcct cgagacggct cagtaacgcg tgggcgagat gagtgtgcga tcccacaggt
     3541 cttctcacgg tacccccgac gcgcacgaca ccctcggtga cccgtccacc ggcaagagga
     3601 atctccggct gttcctccag agtcccgaca acgacgggca agccgcgggg cgcgccacct
     3661 tcgaccaaac tcagcagcgc ctcgcggtcc acgtgatcag ccacgagatc gccgagcttc
     3721 tccagttgag cctcgcggac ggcggcgaag gactcctcgc ccggcctcca gtcgaggccc
     3781 cgctccctgg cgacccaact cagcagggcg cgccggaatc cgtccgattc gaagacgccg
     3841 tgccacgacg taccgagcgt cgcccctgtc aggcagccct ccgtttcatc gccggcatcg
     3901 aaaagaggct cgccgccata acgcctgact cttccgtggt ggatctcgta cccagatact
     3961 gccgcatccc cgaatctgga agcacgcccc ccgggacgtc ccagaacttt gtcttcctcg
     4021 aagaacgtct cgacgggcag gagaccgagt cccatgatct ctggctcgcc gctctcaacg
     4081 ccgtcaacta tacgttctcc gagcatctgg tagccgccgc agataccgag catcggcagg
     4141 ccgagatcag cccgttcggc gagggcgcgg tcgaggccgc gggtgcggag cagcttcagg
     4201 tcatctaccg tcgccttcgt tcccggc
//
LOCUS       DATAIR010000675         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_252923, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000675 DATAIR010000000
VERSION     DATAIR010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252923"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VHM69_15980"
     CDS             complement(<1..1000)
                     /locus_tag="VHM69_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741943.1"
                     /translation="MGDARALEDLGLSIDLYATYVLALDLVVALGFCAVGAVIFRRKS
                     QERGALFVSFALIVFGLTWPDTFDTARLHPVWGGLAGFLTQLGLASLFVFFFIFPDGR
                     FVPRWSRRVVPLVFAMPVLYVLLPGSRLVEPPEPYNILIFLGLWASALLAQVYRYRRV
                     SGPVERQQTKWVVFGATALVVLLIGFLLPLVFFPALDQPGVLSLSTNLIGLTVGGSFG
                     FLLIPLSIGAAILRYRLYDIDVIINRALVYGTLTLTLALTYFGSVALLQYLFRSLTGQ
                     ETQLTVVASTLAIAALFQPLRRRLQNVIDRHFYRKKYDAARTLEAFSDRLRHKTDLE"
     gene            complement(1232..2659)
                     /locus_tag="VHM69_15985"
     CDS             complement(1232..2659)
                     /locus_tag="VHM69_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2741944.1"
                     /translation="MNGQGTYDAVIVGSGPNGLAAAVELARNGQRVAVLEARSTIGGG
                     ARSAEITLPGFVHDLGSAIHPLGYASPFFRSLPLEEHGLEWIHPPTLLAHPFDDGTAA
                     VLERSIEATAATLGPDAGTYRRLMEPVSADAGRIADFFVGRPSVLRHPLALATAGMRS
                     LSDARSLAEKLFATEKARGLFAGNAAHSFLPLEQSPSAMFGILLGTLGHAFGWPFPKG
                     GSQKLADALASYLVSLGGEVYTGVPVRSVDDVPRTRTVLFDVTPRLLLDIAGQHFTER
                     YRSGLKRYRYGPGVFKMDFALDGPVPWEAEECRRAGTVHLGGTLDEISAGESAVARGE
                     HPERPFILLAQHSLFDRTRAPEGKHTVWAYCHVPNGSTVDMTERIEAQIERFAPGFRD
                     IILARRSAGTADLERWNPNLVGGDIVGGSMDLRQLFTRPMPRLNPYSTPAGGLYICSS
                     STPPGGGVHGMCGYLAARVALRYLN"
     gene            complement(2682..>3323)
                     /locus_tag="VHM69_15990"
     CDS             complement(2682..>3323)
                     /locus_tag="VHM69_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741945.1"
                     /translation="LNVDGRRVVGALQGFGQLWQKTYRIRLTGADVSPEEVVRVWKQE
                     FPHFHPPESRFYPSLDGVEPGEVMLINASVQGMPVYTGVMVLYSDEVSFTVMTAEGLP
                     EAGWNTFSAHEEDGTTVVQIQSLARASDPIYEVGFRLFGSTQQEKIWTHVLTTLAARF
                     GVQERVHLEKACVDPQLQWFRIGNLWHNAGLRSVIHAVATQGRRIRDLIRRQG"
BASE COUNT          642 a         1033 c         1084 g          564 t
ORIGIN      
        1 gctccagatc tgtcttatgg cgcagcctgt cggagaaggc ttcgagcgtc ctcgccgcat
       61 cgtacttctt cctgtagaaa tgcctgtcta tgacattctg gagacgcctc cgcagaggct
      121 ggaagagcgc ggctatagcc aaggtcgaag caacgaccgt cagttgcgtc tcctggcctg
      181 tcaaagaacg gaagaggtac tgcagcagcg cgacgctgcc gaagtacgtg agcgcgagcg
      241 taagcgttag cgtgccgtag acgagggcgc ggttgattat tacgtctatg tcgtagagcc
      301 tgtagcgtag tatcgcggcg cctatggaca gggggattag caggaatccg aaggagcccc
      361 cgaccgtcag accgatcagg ttggtggata gcgagagcac tccgggctgg tcgagcgccg
      421 ggaagaaaac tagcggcaac aggaagccga tgagcaacac gaccagagcc gtggcgccga
      481 agacgaccca cttggtctgc tggcgctcca ccggccctga caccctcctg tagcggtaga
      541 cctgcgcgag caaggcactg gcccagaggc cgaggaagat caagatatta tacggctcag
      601 gtggctcaac gaggcgcgag cctggtaaca aaacgtatag caccggcatc gcaaatacga
      661 gcgggacaac ccgccggctc cagcgcggaa cgaaccggcc atcagggaag ataaagaaga
      721 acacgaacaa cgacgcgagt ccgagctggg tcagaaaccc ggccaaccct ccccacacag
      781 gatgcaagcg ggcagtatcg aaggtgtccg gccacgtcag tccgaagact atgagcgcga
      841 aggagacgaa gagagcaccc cgctcttgcg acttccgcct gaagatgacc gcgccgaccg
      901 cgcagaagcc gagcgcaacg acgaggtcca gcgccaggac gtaagttgca tacaggtcga
      961 tcgagaggcc aagatcttcg agtgcgcgcg cgtctcccac ctcgagctgt ccggcgacgc
     1021 atgactcagc agggcacgtc tcgtggaggg ctgcataatg gggaggaatg ctggccgcga
     1081 agataagcgc cgtcaacaga actattacga cccatatggc acgcgcggcg aggagccggc
     1141 ggccgctaag tctggggtgg aagggctcct ggtttcccgt ggtcagggcg gctctgttcc
     1201 gggtcagcgt cgagccctcc ttcccgcgcc tctagttgag gtagcgtagg gctaccctgg
     1261 ccgccaggta tccgcacatg ccgtggacgc ctccacccgg cggggtggac gaagagcaga
     1321 tgtagaggcc accggcaggg gtagagtacg ggttgagtct gggcatcggc cttgtgaaga
     1381 gctgcctcag gtccatggag ccgccgacga tgtcgccgcc gacgaggttg gggttccacc
     1441 gttcgagatc cgccgtccct gcggatctcc tggcgagaat tatatctctg aagcctgggg
     1501 cgaagcgctc tatctgggct tcgatcctct cggtcatgtc gacggtcgag ccgttcggga
     1561 cgtgacagta ggcccagact gtgtgcttgc cctccggggc gcgtgtgcgg tcgaagaggc
     1621 tatgctgggc gagcaggatg aacggtcgct ccgggtgctc cccgcgtgcc actgcgctct
     1681 ctcccgccga gatctcgtcg agcgtgcctc cgaggtggac ggtgcccgcg cgtctgcact
     1741 cctcggcctc ccatggaacc ggcccgtcga gcgcgaagtc catcttgaag acgccggggc
     1801 cgtaacggta gcgcttgagg ccgctcctgt aacgttcggt gaagtgttgt cctgcgatgt
     1861 cgagtagaag gcgcggcgtc acgtcgaaga ggaccgtgcg ggtgcgaggc acgtcatcca
     1921 ccgaacgtac cggtacgcca gtgtagacct caccgcccag agacacgagg taagaggcga
     1981 gggcatcggc gagcttctgc gacccaccct tcgggaaggg ccagccgaaa gcgtgtccca
     2041 acgtccctag cagtatcccg aacatggcgc tcggcgattg ctccagcggc aagaacgagt
     2101 gcgccgcgtt ccctgcgaag agccccctgg ccttctcggt cgcgaacagc ttctcggcca
     2161 ggctgcgggc gtcgctcagg gacctcatac cagccgtagc cagcgccagg ggatgacgta
     2221 atacgctcgg cctgccgacg aagaagtccg ctatcctgcc tgcgtccgcc gagacgggct
     2281 ccatgagcct gcggtatgtc cctgcgtcgg ggccgagcgt ggccgccgtc gcttcgatcg
     2341 aacgttcgag caccgcagcc gtaccgtcgt cgaaaggatg ggcgagcagg gtgggcgggt
     2401 ggatccattc cagaccgtgt tcttcgagcg gcagcgagcg gaaaaacggc gaggcgtagc
     2461 ccaaggggtg tatcgcggag ccaaggtcat ggacgaagcc tggaagtgtg atctctgcgg
     2521 aacgggcacc tccgcctatg gtgctcctgg cctcgagcac ggccacgcgc tgcccgttcc
     2581 gggccagctc tactgcggcg gcgagaccgt tcggtcccga gccgacgatc acggcatcgt
     2641 atgtcccctg tccattcatg ccctgattct agcctcccgc tctagccctg acggcggatc
     2701 aagtcacgta ttcgtcggcc ctgggtggcc acggcgtgga tcacggacct gagacccgcg
     2761 ttgtgccaga ggttgccgat cctgaaccac tgcagctgcg ggtcgacgca ggccttctca
     2821 aggtgtaccc gctcttgcac gccgaagcgt gccgccagag ttgtgaggac gtgggtccag
     2881 atcttctcct gctgcgtcga tccgaacagt cggaagccga cctcgtagat agggtcgctc
     2941 gcgcgcgcca gagactgtat ctggacgacc gtcgtgccgt cctcttcgtg ggcagagaac
     3001 gtgttccaac cggcctcggg gagaccctcc gccgtcatca ccgtaaagga gacctcgtcg
     3061 gagtagagga ccattacccc cgtgtacacc ggcatgccct gcaccgaggc gttgatgagc
     3121 atgacctcgc cgggttccac gccgtcgagc gaaggataga accggctctc aggcgggtgg
     3181 aaatgcggga actcctgctt ccagaccctg acaacttcct ccgggctcac gtccgcgccg
     3241 gtcaggcgaa tcctgtatgt cttctgccag agttgcccga agccctgcag ggcaccgacg
     3301 accctccgtc catcgacgtt cag
//
LOCUS       DATAIR010000676         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_345283, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000676 DATAIR010000000
VERSION     DATAIR010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345283"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VHM69_15995"
     CDS             <1..168
                     /locus_tag="VHM69_15995"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2741946.1"
                     /translation="VNVNGGALALGHPVGASGARILTTLLYELRRRGGGKGIAAICSG
                     GGQGDAVLVEV"
     gene            complement(334..1560)
                     /locus_tag="VHM69_16000"
     CDS             complement(334..1560)
                     /locus_tag="VHM69_16000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2741947.1"
                     /translation="MPQTFVIVGAGLAGGTAASTLRQDGFDGRVVLIGAEKHPPYERP
                     PLSKEYLRGESSFEQALLQPPDYYGQNGIELQPDVRATRIDVREKTVELDGGEYVAYD
                     RLLFAAGVKNRRVPIPGSDLDGVYDLRTVADCNLIRREISPGRKAVVVGMGFIGSEVA
                     ASLRQSGVEVTVIDRNKVPLRRVLGEEVGRVMEVIHRDQGTELVFEDTVAAFGGAGRV
                     ESVTTRAGRHLWCDFVVIALGVEPAAEPLAGTGVKIDNGVVVDEYCRTGVEGIYAAGD
                     VANQYHPVFGRHIRVEHWQNAIMQGQAAARSMMGVGEPYQEIPWFWSDQYEHNLQYVG
                     FHREWDELVVRGSLEDRSFVAFYRKDERVLAAVAIDRGRDLRRSVPLIKARERIEAAR
                     LRDIDTDLRTLVDARP"
     gene            complement(1560..1868)
                     /locus_tag="VHM69_16005"
     CDS             complement(1560..1868)
                     /locus_tag="VHM69_16005"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX2741948.1"
                     /translation="MTGFITIAGADELEAGEMRAFDVRGVKIAVANVSGAFHAFDDTC
                     THAGCSLAGGDLEETTVICACHGSEFDVRSGEVLRGPAREPVATYETRVEDGRLEIED
                     "
     gene            complement(1891..2394)
                     /locus_tag="VHM69_16010"
     CDS             complement(1891..2394)
                     /locus_tag="VHM69_16010"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX2741949.1"
                     /translation="MESKAKIMGHPIHPILIPFPPGLLMTSVVFDIVYLITGGGRWAE
                     IIQKEISAGVIGGLIAAVFGLIDWLAIPSGTRAKAVGLLHGLTNCLMVALFAIGWLLR
                     AGVPGEPGAAAIVLSFAGVGVASIGGFLGASLSYAWVLGYPKGRTSTRRVRSPDVRRA
                     ESGMESC"
     gene            2525..3181
                     /locus_tag="VHM69_16015"
     CDS             2525..3181
                     /locus_tag="VHM69_16015"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2741950.1"
                     /translation="MAVLWDRKFFESTRGRLVTLLRRRGRTVDELAADLGLTNNGVRA
                     HLATLERDGLVRRGGSVRRVGGGGKPAYVYELTSEARELFPRAYEPVLGGLLDVLLDH
                     FGSEELEKLLREAGTRMASGGSVPSGGIRERLEAAVGVLDELGGLAEIEDREGTFVIR
                     SYGCPLAAVVPKQPRACDLVETLIATLAGVRVRERCERGENPRCRFDVGPADGFTGST
                     "
     gene            3339..3809
                     /locus_tag="VHM69_16020"
     CDS             3339..3809
                     /locus_tag="VHM69_16020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX2741951.1"
                     /translation="MSDETPDNPGVIAPPPLIYAAALAAGLLANRRYRIPFLPRPLTR
                     TLGWPLVIGGIAIGFLGSREMRRVETNLDPYKPTTAIVSDGPFRFTRNPLYLSMTLIY
                     CGITALANALPAALLLPAVLYLMRRGVIEREERYLERKFGDEYLQYKARTPRWI"
     gene            3800..4339
                     /locus_tag="VHM69_16025"
     CDS             3800..4339
                     /locus_tag="VHM69_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX2741952.1"
                     /translation="MDLNENSNLLPHGEKFPEVAESAWIAPGAYVIGDVYLGEQSSVW
                     YGAVLRGDTEPIRIGARTNVQDGCVLHADPGYPAVVGEGCVVGHKAVVHGCQIGANCL
                     VGMGATILNGAKIGEGSIVAAGAVVPEGREFPPRSLIVGIPAKRIGDVSEEQTADIAR
                     GAGEYVERARAHKESLARG"
     gene            4637..5161
                     /locus_tag="VHM69_16030"
     CDS             4637..5161
                     /locus_tag="VHM69_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2741953.1"
                     /translation="MPIDHIETQRLHMRAFQPNDWQAVYDYTSDSAVMMYIPEGPFTS
                     EQAKTFVADNMGEQAHAVAVLLKTADMLVGHMVFHPWFAPQTYEIGWVFNSAYHGQGY
                     ATEAAVALLQYGFEALHLHRIIATCQPENVASWRVMEKLGMRREAHFRKCIRRPGNQW
                     WDEYFYALMEEEWF"
BASE COUNT         1078 a         1672 c         1595 g         1087 t
ORIGIN      
        1 gtcaacgtca acggcggcgc gctggcgctt ggccatcccg taggcgcgtc cggcgcgcga
       61 atcctgacta cgctgctcta cgagctacgg cgcaggggag gcggcaaggg aatcgcggcg
      121 atctgctccg gcggcggaca gggtgacgcc gttcttgtgg aggtctgaca gcggcttggg
      181 gaaggattga tcctccccaa gccaggcttc aggtttcagg catcaggaag gtcttgccgg
      241 acggacggtg gtcgctaagc tgcacctctg gggcgaggtg gatcaatact tatctactgc
      301 gctgtgagaa gggctgcagc gtcagtattc gactcatgga cgcgcatcaa cgagcgttcg
      361 caggtcggtg tcgatgtcgc gaagccttgc ggcttcgatc ctttcgcggg ctttgatcag
      421 gggcacagag cggcgcaggt ccctccccct gtctatcgcc acggccgcga gaactcgttc
      481 atcttttcgg tagaacgcga cgaagctccg gtcctccagg ctgcctcgca cgacaagctc
      541 atcccattct ctgtggaacc cgacgtactg gaggttgtgc tcgtactgat ctgaccagaa
      601 ccacgggatc tcctggtaag gttcaccgac tcccatcatg cttctggccg ccgcctgacc
      661 ctgcatgatc gcgttctgcc agtgctcgac gcggatgtgc cgaccgaaca cgggatggta
      721 ctggttggcc acgtcgcctg cggcgtagat tccctcgacg cccgtccggc agtactcatc
      781 cactacgacg ccgttgtcga tctttacccc ggtgcctgcg aggggttctg ccgcaggctc
      841 gacaccgagg gcgatcacga cgaaatcgca ccagaggtgg cggcctgccc tggtcgtgac
      901 gctttcgacg cgacccgcac ccccgaaagc cgccaccgtg tcctcgaaga caagctccgt
      961 tccctggtcg cggtgtatga cttccattac acggccaacc tcttcgccga ggacccggcg
     1021 taacggcacc ttgttacggt ctattacggt tacctccacg cccgattgcc tgagcgaggc
     1081 tgccacctca gagccgataa agcccatccc aacgacgacc gccttgcggc ctggtgagat
     1141 ctcccggcgt atgaggttac agtcagccac cgtgcgcaga tcatagacgc catccaggtc
     1201 cgagccaggg ataggaacgc gcctgttctt cactccagcg gcgaagagaa gcctgtcata
     1261 agcgacgtac tctccgccat ccaactcgac ggtcttctcc ctgacatcta ttcgtgtagc
     1321 ccgaacgtcg ggctgcagct cgatgccgtt ctgaccgtaa tagtcgggcg gttggagcag
     1381 agcttgctcg aacgaggact cgccgcgcag gtactctttg gagagcggag gccgctcgta
     1441 gggcgggtgc ttctccgcgc cgatcaagac caccctgcca tcgaaaccgt cctggcggag
     1501 ggtagatgca gccgttcccc ccgccagccc ggcgccgacg atcacgaatg tctgaggcat
     1561 caatcctcga tctctagtct gccgtcctcg accctggtct cgtaggtcgc gactggctcc
     1621 ctggccggcc cccggagcac ctcaccgctt ctaacatcga actcggagcc gtggcacgcg
     1681 cagatcacgg tggtctcttc gagatctccg ccggcaagcg aacacccggc atgcgtacag
     1741 gtgtcgtcga acgcgtggaa cgcgccggac acgttggcaa cggcgatctt gacgccccgc
     1801 acgtcgaatg cccgcatctc acctgcttct aactcgtccg ctccggctat ggttatgaaa
     1861 ccggtcatcg ctaactcctt tcattgacct tcaacaactc tccattccac tctccgcccg
     1921 ccggacgtcc ggagagcgaa ctcggcgcgt tgaggttcgc cccttcggat accccaacac
     1981 ccatgcgtac gacaagctcg ccccgagaaa acctccaata gaagcaactc caacacctgc
     2041 gaacgaaagc acgatggcag ccgctcccgg ctcgcccggc acccccgcgc gcaacagcca
     2101 accaatagcg aacagcgcca ccataagaca gttggtcaag ccatgcaaca atcccaccgc
     2161 cttggcgcgc gtccctgaag gtatcgccag ccagtcgatc aaaccgaata ccgccgcaat
     2221 aaggccaccg atcacaccgg cggagatctc cttctggatg atctccgccc accgcccgcc
     2281 accagttatc aggtagacga tgtcgaagac tacggaagtc atgagtagac ctggaggaaa
     2341 agggatcagg atcgggtgta tcggatggcc cattattttc gctttgctct ccatattctc
     2401 ctttcccgga tcctgtccgc gcacgataca gcaatatcaa aaataagtca aataattact
     2461 tgatttattt gagtatttat tcttactctg ttacagtagg gttgtaatag agaaggggtg
     2521 gctattggca gttctctggg accgcaagtt tttcgagagc acgcgtgggc ggctcgtcac
     2581 gttgctcagg cgtaggggac gcacggtcga tgagcttgcc gcggatctgg ggctgacgaa
     2641 caacggagtt cgggcgcatc tggctacgct ggagcgggac ggcctcgtgc ggcggggtgg
     2701 ttctgtgcgt cgcgtcggcg gtgggggcaa gcctgcttac gtctacgagc tgacttccga
     2761 ggcgcgagag ttatttccca gggcttatga gccggtgctt ggtggactcc tggatgtctt
     2821 gctagatcat tttgggtctg aggaactgga gaaactacta agggaagcgg ggactcgcat
     2881 ggcgagtggg ggcagtgtgc cttcaggtgg tatccgggag cggctcgagg cggccgtagg
     2941 ggtgctcgac gaactggggg gactcgccga gatcgaggat cgcgaaggca ctttcgtcat
     3001 ccgcagttat ggatgtccgc ttgcggcagt ggtgccgaag caaccccggg catgtgatct
     3061 ggtcgagacg ctcattgcga cgcttgcggg tgttcgcgtc cgcgagcgct gcgagcgcgg
     3121 tgaaaatcct cgctgccgct tcgatgtggg tccggccgat ggtttcacag ggagtacgta
     3181 gagtctatcg acgggactcg ggtggttgtg cacgagaaag cgtggcatca tcactggtct
     3241 ggcgcggctt tagaggtact gacgtttacg tccgcccgag gcaggatctg tccgcgtaga
     3301 gctgtgtgag caggccatcc acaaaaggag ttatcgaaat gagcgatgag acgccagaca
     3361 atcccggtgt gatcgcacct ccgcccctga tctacgccgc tgctcttgcc gccggtctgc
     3421 tggcgaaccg ccgctaccga attcccttcc tgcctcgtcc actcacgaga acgctcggct
     3481 ggccgctggt catcggcgga atcgcgatcg gatttctcgg atctcgcgag atgcgccggg
     3541 tcgagacgaa cctggacccg tacaaaccga cgaccgccat cgtctccgat ggcccattcc
     3601 gcttcacgcg caatcctctc tatctgagca tgaccctgat ctactgcggc atcaccgccc
     3661 tcgcgaacgc gctacccgcc gcattgctgc tccccgctgt tctttacctc atgcggcgcg
     3721 gcgtcatcga gcgcgaggag cgatacctgg aacgcaagtt cggcgacgag tatctacagt
     3781 acaaagcgag gactccgcgt tggatctgaa cgagaatagc aacctgctcc cgcacggcga
     3841 aaaattcccc gaagtcgctg agtccgcgtg gatcgcacca ggagcctacg tcatcgggga
     3901 cgtttatctg ggtgagcaat cgagcgtctg gtacggggcc gttctgcgcg gcgacacgga
     3961 gccgatccgc atcggtgcgc ggacgaacgt ccaggacggc tgcgtactcc acgccgaccc
     4021 cggctaccct gcggtcgtgg gcgaggggtg cgtggtcggc cacaaggcgg tcgtgcacgg
     4081 ctgccagatc ggggccaact gcctcgtcgg tatgggagcc accatcctca acggcgcgaa
     4141 gatcggcgaa ggctccatag tcgccgcggg agccgtcgtc cccgagggcc gcgaattccc
     4201 tccccgcagt ctcattgtcg gcatccccgc taaacgcatc ggcgacgtga gcgaggagca
     4261 gaccgctgac atagcgcgcg gagccggcga gtacgtcgag cgcgccagag cccataagga
     4321 gtcgctggca cgcggctgac acgcctcccg cgttcggcca aaccctagat ccaccccgcc
     4381 aggaacaact agcttcctag ggtgtatatt gcgtatgggt tttgtttcga ctagacttcc
     4441 catcagagtc gttggggcaa catagtgggc gtaaacgatg accggacagg gcaagcctac
     4501 agcaagacgt aacgggcatc gcggcataac tcctttccga gaacgttata ctgcacatag
     4561 cggcctagct aatagttggg cgtctgtctc gcgatggcag cccacctaat tagaaactgt
     4621 tctagagaga cctagaatgc cgattgatca catcgaaacg caacgattac acatgcgcgc
     4681 tttccagcct aacgactggc aagccgttta cgattacacg tccgactcag ctgtcatgat
     4741 gtatatccct gaagggccat ttacgtctga acaagccaag acgtttgttg ccgacaatat
     4801 gggcgaacag gcgcacgccg ttgctgtcct cctcaaaaca gccgatatgc tcgttgggca
     4861 catggtgttt catccgtggt ttgctcctca gacttatgag attgggtggg tcttcaatag
     4921 cgcctatcac ggccaagggt atgccacgga agccgctgtg gcattgctcc aatatggctt
     4981 tgaagcgttg cacctgcatc ggatcattgc tacctgtcaa cccgagaacg ttgcttcgtg
     5041 gcgggtaatg gaaaagcttg gcatgcgacg agaggcccac tttcgaaagt gcatcaggcg
     5101 tccgggtaat cagtggtggg acgaatactt ctatgctctt atggaagaag agtggttcta
     5161 ggtcaatgag acataaccgg ggagcgtaca cggggaagtc cgcccaactc atgcgccggc
     5221 caacaaaggc atacagccta cagcttcggg ccgcgcctga tgagctgacc cggcaaacag
     5281 ttcaacccag cagggtctta ccctgccgtt cgtctgacaa gccctagcct ccgaaagcct
     5341 cgtggtgcca ccggcttcac tgatctgctc ctagtacact cgtccttagc gtaccggtgg
     5401 ggccaataat agcttcctaa cacaaattct cc
//
LOCUS       DATAIR010000677         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_345422, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000677 DATAIR010000000
VERSION     DATAIR010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345422"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..703)
                     /gene="mreD"
                     /locus_tag="VHM69_16035"
     CDS             complement(188..703)
                     /gene="mreD"
                     /locus_tag="VHM69_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX2741954.1"
                     /translation="MEQASVVRAAVVVALAALLDSVVGPYLTFGFFSPKLTLIAIVFV
                     VSTLRDLQAVLLGFFGGILLDALGSDLFGVGALGGLLAATLSARASAVRRKGTERVLL
                     TQVVALAVAGYDLMGFTARWLAGLGVPTLGEYAVWGVFPDVAFNALMAFLVGGWLLKV
                     VSVKPHGWESN"
     gene            complement(713..1642)
                     /gene="mreC"
                     /locus_tag="VHM69_16040"
     CDS             complement(713..1642)
                     /gene="mreC"
                     /locus_tag="VHM69_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX2741955.1"
                     /translation="MGRRKSSAASSLAALVILCLASVALFTVYVKEGTCAREGECGPL
                     HTVQLGAAEVLQPVRSVFATVFEPLQETSESVMSAFDNSQEERLQRELRETEALAAEA
                     SRLERENAHLRELLNGARSSYEYGPLARVVAPVGDQFTQRIVINVGTEDGVEPNKPVV
                     IGKSTLVGRTTEVSKHTAQVMLVTDQMFAAGVKIIPPAQFDPASDELSPAVTQENVSY
                     GQGLLETNLEGYFGVDLVNLSARAEEGDYVVTSGRAGNRNLLFPPGLYIGTVESVSSQ
                     DYNQFKKIVVDPAMNPQDLEEVRVIVDWARRDG"
     gene            complement(1649..2677)
                     /locus_tag="VHM69_16045"
     CDS             complement(1649..2677)
                     /locus_tag="VHM69_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2741956.1"
                     /translation="MFGGLFGRDVAIDLGTANTLVFVKGQGIVLSEPSVVAIDTKTDK
                     VVAVGSAAKRMLGRTPGNIVAMRPLKDGVIADFEVTEKMLAYFIRKVQPQRLFFRSLV
                     GPRVVVCVPSGVTGVELRAVKEATESAGARQAYTIEEPLAAAIGASLPVNEAQGSMVV
                     DVGGGTSEVAVISLGGIVTKSSIRIAGDDIDDAITTYIQKEYKLAIGTQTAEQLKIEL
                     GSAFRLEEEESAEIRGRDLVTGLPKTIVITSEEIREAISIPVDAMIAAVRDTLDRTPP
                     ELASDIMDRGMVLAGGGALLRHLDERLRRETGIPVHVADDALMCVAIGSGRSLEEINS
                     YRSALSSA"
BASE COUNT          489 a          901 c          804 g          487 t
ORIGIN      
        1 gatcaacgtg gggatggcga tgggcatgat gcccgtgaca gggctgcccc tgcctttcat
       61 aagctacggg cgcagcaacc ttctcgtgag catgatgtcc gtcgggctcg tccagagcat
      121 cgtgatccag gcgaggctca aaactgaagg gaacccacgc ccctgagcag cccggaagtc
      181 tccaaagtta gttactttcc cagccgtggg gcttgacgct cactaccttc agcaaccagc
      241 caccaacgag gaacgccatc agcgcgttga aagcgacgtc ggggaagact ccccacacgg
      301 catactcacc gagggtcgga acgccaagac cggccagcca gcgagcagtg aatcccataa
      361 ggtcgtagcc tgcgacggcg agggctacaa cctgggtgag cagcacccgc tccgttccct
      421 ttcgccgcac ggcgctcgcg cgggccgaga gcgtggccgc gagcaagccc ccgagcgcgc
      481 ctaccccgaa gaggtcgctc ccgagggcgt cgagcagaat tcccccgaag aagccgagca
      541 acacggcctg cagatcccgc agcgtcgaga ccacgaagac tatggcgatc agggtcaatt
      601 tcggagagaa gaagccgaag gtcaggtatg gtccaacgac ggagtcaagg agcgcggcca
      661 gagccacgac gacggcggca cggacgacgg aggcctgttc cacggctggt ccctacccgt
      721 cccgacgggc ccagtcgacg atgacccgca cctcttcgag gtcctgcggg ttcatggccg
      781 gatcaacgac tatcttcttg aactgattat agtcctgcga gctcaccgac tctaccgtac
      841 cgatataaag accgggaggg aacagcaagt tcctgttgcc tgcccgcccg ctggtcacca
      901 cgtaatcgcc ctcctcggca cgggcgctga ggttcacgag gtccaccccg aagtaccctt
      961 cgaggttggt ctcgagcagc ccctggccgt aggagacgtt ctcctgggtg acggccgggc
     1021 tcagctcatc cgacgcgggg tcgaactggg ctggagggat gatcttgacc ccggcggcga
     1081 acatctgatc tgtgacaagc atgacctggg ccgtatgctt ggagacctcc gtcgtgcgcc
     1141 cgaccagggt gctcttgccg atcacaacgg gcttgttagg ctcgactccg tcctctgtgc
     1201 cgacgtttat tactatcctc tgggtgaact gatccccgac cggcgcgact acccgcgcga
     1261 gcggcccgta ctcgtacgag gacctcgcgc cgttcaataa ctcgcgcagg tgcgcgttct
     1321 cgcgttcgag ccgcgacgct tcggcggcca gggcttcggt ctcccgcaac tcgcgctgga
     1381 gcctctcttc ctgactgttg tcgaaggcgc tcatcacgct ctcactcgtc tcctgaagcg
     1441 gctcgaagac ggtagcgaag acgctcctta ccggctgaag gacctctgcg gcgccgagtt
     1501 ggaccgtatg caacggtccg cactctccct cgcgcgcaca ggtgccctcc ttcacgtaga
     1561 cggtgaagag cgccacgctg gccagacata ggatgacgag ggccgcgaga ctgcttgcgg
     1621 cactggactt cctccgaccc acgtcgcgct aggccgagga gagagcgcta cggtaggagt
     1681 tgatctcttc gagcgagcgc ccgctgccga tggcgacgca catgagcgcg tcgtcggcga
     1741 cgtgaacggg gatgcccgtc tcgcgcctca gccgctcgtc gaggtgcctc aagagcgccc
     1801 cgccaccggc caggaccatc cccctgtcca tgatgtccga tgcgagctcg ggaggcgtgc
     1861 ggtccagggt gtcccgaact gccgcgatca tcgcgtcgac agggatgctg atggcctccc
     1921 tgatctcctc gctcgtaatt actatggtct tgggcagccc cgtgacgagg tccctgcccc
     1981 ttatctccgc cgactcttcc tcttcaagcc tgaaggcgct gccaagctcg atcttgagct
     2041 gctcggcggt ctgggtgccg attgcgagct tgtattcttt ctgtatgtaa gtcgtgatgg
     2101 cgtcgtcgat gtcgtcgccg gcgatgcgga tggaggactt ggtgactatg ccgccaaggg
     2161 atattacggc tacctcgctc gtccccccgc cgacgtctac caccatggac ccctgcgcct
     2221 cgttgacagg cagactggcc ccgatggccg ccgccagagg ctcctctatg gtgtaagcct
     2281 ggcgggcgcc cgccgactcc gtcgcttcct tcactgccct caactccacg cccgtcacgc
     2341 cagaggggac acatacgacc accctcggac cgacgagcga cctgaagaac aggcgctgtg
     2401 gctgcacctt gcgaatgaag tacgcgagca tcttctccgt gacctcgaag tcggcgatca
     2461 caccgtcctt caggggtctc atagcgacga tgttgcccgg cgtgcggccg agcatccgct
     2521 tcgccgcaga tcccacggcg acgactttat ccgtcttggt gtcgattgct actaccgagg
     2581 gctcggagag tacgatcccc tgccccttga cgaacacgag cgtgttcgcc gttcccaggt
     2641 ctatcgcaac gtcccgcccg aaaagtcctc caaacatacg c
//
LOCUS       DATAIR010000678         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_207624, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000678 DATAIR010000000
VERSION     DATAIR010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207624"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VHM69_16050"
     CDS             complement(<1..180)
                     /locus_tag="VHM69_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741957.1"
                     /translation="MKEGMGLGDIAPGLIVRHTNRRLGKLDRCEACERLISGRISFSE
                     FVDQVGEEYRELLDFM"
     gene            413..1702
                     /locus_tag="VHM69_16055"
     CDS             413..1702
                     /locus_tag="VHM69_16055"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2741958.1"
                     /translation="MSVRREARVVVVGAGFAGASLIRHLPPVLRRPGETLLIDRKEEY
                     TFVPLIHEVAVGRLHPKSVRSPIGPLCPGACEFVQTEVLGIDLSNKTLETADGTISYE
                     YLVLNPGSVAAEPGPRMQKFFQPFLSLDDALLLRSTLSDAWHTATRPGPPPPPGILTV
                     AIVGGGATGVELAAEVATLFAYLGKRSHRRPSEEPRVVLLEATFRLMGWLDPYFDRVA
                     MRTLSDLGVEVRLNSHVEEASADGIRAGKYWLKAGTRVWTTGVEASPLARSLPGEHDA
                     TGRAYVDQHLTLPDHPEIYVLGDAAVYTDPRLGPLPPTASVAVQQGPWVARDLKRRLR
                     GAGSRRGRPPFRFFDRGYVVSLGPDNAVADAVGLRLRGAPAQALYRSVLLYYMKSRRD
                     RILTGADWAMERTLGRVGFGSRQPSSDGGEEDLEGEG"
BASE COUNT          297 a          582 c          555 g          303 t
ORIGIN      
        1 catgaaatcg agtagctccc tgtactcttc gccgacctga tccacgaact ccgagaacga
       61 gatgcggcca gagatcaaac gctcgcacgc ttcgcagcgg tcgagcttcc ccagacgacg
      121 gttggtgtgc cttacgatga gtccgggggc tatgtccccg agacccattc cctccttcat
      181 caccaagacg gtccctttct agccccatag gggtcgttcc ccgttttcca tatacacccc
      241 gtatatcgcc agcgccccat ccgccggcgg tccgtgcagt ctacgttcgg gcgcgccttt
      301 tgtaaagcgc atcgccagga ccctgcgccc gaaggatata cttccggcaa agatcaggta
      361 tcgtgaagga gggtcccgtg ccaggagagt gctatcgcag gaggcccgta ccgtgagcgt
      421 caggcgcgaa gctcgcgttg tggtcgtcgg cgcgggcttc gccggagcct cactgatcag
      481 gcacctcccg cctgttctgc ggaggccggg cgagacgttg ctgatcgacc gcaaggaaga
      541 gtacacgttc gtgcccctga tccacgaggt cgccgtgggc cgcttgcatc ccaagagcgt
      601 ccgctcaccg atcggccccc tctgtcccgg ggcctgcgag ttcgtgcaga ccgaggtatt
      661 ggggatcgat ctcagcaata agacgctcga aaccgccgac ggcaccatca gctacgagta
      721 cctggtactc aaccctggta gcgtcgccgc ggagccaggt ccgcgcatgc aaaagttctt
      781 ccagccgttc ttgagcctgg acgatgcgtt gctcctgcgc agcaccctga gcgacgcctg
      841 gcatacggcg acgaggcctg gtcctccgcc acctccgggc atccttaccg ttgccatcgt
      901 cggcggtggg gcgacaggcg tggagctggc cgccgaggta gcaaccctct tcgcttacct
      961 cgggaagcgc tcccaccgca ggccatcaga ggaaccgcgc gtcgtgctgc tcgaggctac
     1021 gttcaggctg atgggctggc tcgacccgta cttcgaccgc gtcgcgatga ggacgctctc
     1081 cgacctcggc gtcgaagtgc gcctgaactc ccatgtagag gaagcgagcg cggatggaat
     1141 ccgggccgga aagtactggc tgaaggctgg cacgcgggtg tggacgaccg gagtcgaggc
     1201 gtcgccgctg gcacggagcc tgcctggaga gcacgatgcg acgggccgcg cgtacgtgga
     1261 ccagcatctc accctcccgg atcatcccga aatctacgta ctcggcgacg ccgcagtata
     1321 cacagacccg cgcctcggtc ctctgccccc caccgcttcc gtcgctgtgc agcagggacc
     1381 gtgggtcgcc cgtgacctca agcgccggtt gcgaggagcc gggagccggc gcggcaggcc
     1441 gcccttccgc ttcttcgacc ggggctacgt cgtgagcctg ggtccggata acgccgtggc
     1501 cgacgccgtt ggcctgaggc tgcgtggcgc cccggcgcag gccctctatc gcagcgtgct
     1561 cctctactac atgaagagta ggcgcgacag aatactcacg ggcgccgact gggcaatgga
     1621 gcggaccctt ggccgcgtgg gtttcggtag ccgccagcca tcatccgacg gcggtgaaga
     1681 agacctcgaa ggcgagggct gaccggggtt cctgtggatc ggcaacgtga cggctgc
//
LOCUS       DATAIR010000679         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_68356, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000679 DATAIR010000000
VERSION     DATAIR010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68356"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VHM69_16060"
     CDS             <1..954
                     /locus_tag="VHM69_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX2741959.1"
                     /translation="LARMGLKHINRGLAVGGARPGIEALVKVAGVRGELDEGDLGWKL
                     GPRINSIGRIKNPRPALDLLLTDDRREAFRIASLLNQLNSERQRRTKFAVERAMAEVD
                     PEQDFKVVVTEETGGLAGLIAGRVAGATGRPAAILTRRADDSYGGSARAGETDVDLYG
                     ALYSVRHLMGEWGGHRKAAGLSVNPGNLDAFVAGVNEAVRKQSVEKPEVFVPAIEIDA
                     EIPLGSVSNGLLDWHEMLAPFGSGNYRPIFVTEGLRIAGSRKIWEGMNLIEVEGGIKA
                     KMAGEPDGLPAGPFDAAYTVGRSRYSGKAELEIVDWRQTGS"
     gene            951..1904
                     /locus_tag="VHM69_16065"
     CDS             951..1904
                     /locus_tag="VHM69_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2741960.1"
                     /translation="MNAIGVDVGGTKIAAGVVTPEGKILDEVRYPTPHSPQTLVDTIA
                     RALTEVGDGYEVGGACLAVPGLILARENKVVFSPNLRAIEGIPLKDALEPKIGMPLTI
                     DNDGNAAAWGEFRFGVGSEADHLVFVALGTGIGGGVITHGILMRGAQGSGGELGHVTI
                     QATGPRCACGNHGCLEALASGTAIGRRARELAIESPDSALGRLAMRRQILGEDVTELA
                     REGDKVALSVLEEAGTWLGVGLAGFVNVFNPEVVAVGGGVMEAGELVLEAARREVILR
                     SRPPSRDLAEVKVATLGPRSGVLGAAALARDASSGECVLDA"
     gene            1904..2704
                     /gene="rsmI"
                     /locus_tag="VHM69_16070"
     CDS             1904..2704
                     /gene="rsmI"
                     /locus_tag="VHM69_16070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2741961.1"
                     /translation="MPLTVIPTPIGNLEDITLRALRHLREADLVACEDTRRTGRLLAH
                     YEIRKDLVAYHEHNEERLAPELAERAKTERVSLVTDAGTPLVSDPGYRLVRACIEAGV
                     EIEVLPGPSALTTALVASGLPADTVVFTGFPPRKGKERTQLLGRIATEPSTFVLFESP
                     HRLGKTLGDLPAGTPVAVCRELTKLHEEVFRGKTEEAARHFSGGVKGEIVLVIQGGTA
                     KAEESIEEVVRRARGYVSEGESPSQAAARAARESGSRRGEVYERLVKG"
     gene            2793..3551
                     /locus_tag="VHM69_16075"
     CDS             2793..3551
                     /locus_tag="VHM69_16075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2741962.1"
                     /translation="MLVDSHTHLLRLEVSPEEAVKDAAEAGVGAVVNIGTDVEDSRRG
                     AELAAVLPNVYSTAGIHPHNAGTYTAADLEALAELSESPGIVALGEVGLDYYRSEWSG
                     EVQRALFEDVISLANDTRLPLVIHSRHAFADTMAALGSARVPIVLHCFEGGDREVREA
                     RERGYYVGRAGNVTYENSETARVLRLIDEERILVETDAPYLSPQPLRGRKNAPKNVVH
                     TASFVAERLGLEDEELATLTTRNARNLFGLPLEV"
     gene            3558..>4172
                     /gene="rsmA"
                     /locus_tag="VHM69_16080"
     CDS             3558..>4172
                     /gene="rsmA"
                     /locus_tag="VHM69_16080"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX2741963.1"
                     /translation="MSEQGPPKKRLGQHFLKDPNTARIVASGVKKDDVVLEVGPGRGF
                     LTAFLAEKARLVHAVELDPDVLPSLREAVGDMDNVLIHEGDALRFDYSTLDPAPNKLV
                     ANLPYNIASPLVLRFLEEVDSLTTLRFMVQLEVARRMAAERGTKDYASYAVLAQLLAR
                     VRISHKVPPTVFDPPPRVHSAVVEMERREPPDDYERIKSVVLGAF"
BASE COUNT          760 a         1318 c         1430 g          664 t
ORIGIN      
        1 ctcgcccgca tggggctcaa gcacataaac agggggcttg cggttggcgg agcccgtccc
       61 ggcatcgagg cgctcgtcaa ggtggccggg gtgaggggcg agctggacga aggggacctc
      121 gggtggaagc taggtccgcg catcaactcc atcggaagga taaaaaaccc tcgtcctgct
      181 ctggatctgc tcctcaccga cgaccgtagg gaggccttca gaatcgcttc cctcctcaac
      241 cagctcaact ccgagcggca gcggcggacg aagttcgccg tcgagcgggc catggctgag
      301 gtggatcccg agcaggactt caaggtcgtc gtgacggagg agacgggggg gcttgcgggc
      361 ctgatcgcgg gccgcgtcgc tggcgctacg gggcgtccgg cggcgatcct gacccgcagg
      421 gccgacgact catacggcgg ctcggcccgc gctggcgaga ccgacgttga cctctacggc
      481 gccctctact ccgtcaggca tctgatgggg gagtggggcg gccaccgcaa ggccgcgggt
      541 ctctccgtca accctggcaa cctcgacgcc ttcgtcgccg gcgtcaacga ggccgtcagg
      601 aaacagagcg tcgagaaacc ggaggtcttc gtccccgcca tagagattga tgccgagata
      661 ccgcttggga gcgtatccaa cggtctgctc gactggcacg agatgctcgc cccgttcggc
      721 agcgggaact atcgtcccat cttcgtcact gaaggactca ggatcgcggg ctcgcgcaag
      781 atctgggagg gcatgaacct catcgaggtg gaaggcggga tcaaggcgaa gatggccgga
      841 gagcctgacg ggttgcccgc cggcccattc gacgcggctt acacggtcgg ccgcagccgg
      901 tactcaggca aggcggagct ggagatagtg gactggaggc aaaccggctc gtgaacgcca
      961 taggggtgga cgtcggaggc accaagatcg ccgctggggt cgtcactcct gagggcaaga
     1021 tcctggatga ggtacgctac ccgacgccgc actcgccgca gacgctggtc gatactatcg
     1081 cccgcgccct tacggaggtc ggggacggct acgaggtcgg tggtgcatgc ctcgccgtgc
     1141 ccggcttgat cctcgcccgg gagaacaagg tcgtcttctc cccgaacctg cgcgccatcg
     1201 agggcatccc cctgaaggac gcgctcgaac cgaagatagg gatgcccctc acgatagaca
     1261 acgacggcaa cgccgcggcg tggggcgagt ttcgttttgg cgtgggaagc gaggccgacc
     1321 acctcgtctt cgtcgccctg ggaacaggga tcgggggagg tgtgatcaca cacggcatcc
     1381 tgatgcgcgg cgcgcagggc tccggcggag agcttggaca tgtgacgatc caggccacgg
     1441 gaccccgatg tgcatgcggc aaccacggct gcctggaggc cctcgcctcg ggcaccgcaa
     1501 taggacgccg cgcccgcgag ctcgcaatag aaagtcccga ctcggccctg ggacgcctcg
     1561 ccatgcggcg ccagatactc ggcgaggacg tcaccgagct agcccgcgaa ggagacaagg
     1621 tggcgctctc ggtgctcgag gaggccggaa cctggctcgg cgttggcctc gccggcttcg
     1681 taaacgtctt caaccccgag gtggtcgccg tcggtggagg cgtcatggag gcgggagagc
     1741 tcgtcctcga agcggcccgc agggaggtga tcctgcgttc caggcctcct tctcgcgacc
     1801 tcgcggaggt gaaggtggcg accctcgggc ccaggtccgg cgtcctcggc gccgcagccc
     1861 tcgcccggga cgcatcttcc ggggagtgcg tgctggacgc ctgatgcccc tcacggtgat
     1921 cccaactccc ataggcaacc tggaggacat caccctccgc gccctcagac atttgcgcga
     1981 ggccgacctc gtcgcctgcg aggacacccg tcgcacagga cgcctgctgg cacactatga
     2041 gataaggaaa gacctcgtcg cctaccacga acacaacgag gagcgcctgg cccccgaact
     2101 ggccgaaagg gcgaagaccg agagggtctc gctcgtcacc gacgcaggca ccccactggt
     2161 ctccgaccct ggttatagac tcgtccgtgc ctgcatcgag gccggtgtgg agatagaagt
     2221 cctcccagga ccctcggccc tcaccacggc cctggtggcc tcagggcttc ccgccgacac
     2281 ggtcgtcttc accggcttcc cgccgcgcaa gggaaaagaa cgtacgcagt tgctcgggcg
     2341 tatcgcgacc gagccctcca ccttcgtgct cttcgagtct ccgcaccggc tcgggaaaac
     2401 gctcggtgat cttccagccg ggacccctgt cgctgtgtgc agggagctca ccaagctcca
     2461 cgaggaggtc ttcaggggga agacggagga ggccgcgcgg cacttctcgg gtggtgtgaa
     2521 gggtgagatc gtgctggtga tccagggcgg aacggcgaag gcggaggaga gcatcgagga
     2581 ggtagtcagg cgggcgcgag gatacgtgtc agagggggag agtccttctc aggctgcagc
     2641 gagggcggcc agggagtcag ggtcgcggcg cggtgaggtc tatgaacgtc tggtaaaggg
     2701 ctgaaggaag atttggatgc ttccggcctg acgcctgaga gccgacgcct ttataatgcc
     2761 cctcgctgag aactcttggg gagaggggcg ctatgctcgt tgactcgcac acgcaccttt
     2821 tacggttgga agtctcgccg gaggaagcgg tgaaggacgc cgcggaggcg ggggtcgggg
     2881 ctgtggtgaa catcgggacc gatgtcgagg actcgcggcg cggggccgag ctcgcggcgg
     2941 tattgccgaa cgtctactcg acggcgggga tccatccaca caacgccggt acgtacacgg
     3001 cggccgacct cgaagcgctg gccgagctct cggagtcgcc cgggatagtg gccctcggcg
     3061 aggtcgggct tgattattac aggtccgagt ggtccggaga ggtgcagcgg gcgcttttcg
     3121 aggatgtgat ctcactcgcc aacgatacgc gcctgccgct ggtgatccac tctcgccacg
     3181 ccttcgcaga cactatggca gccctcggga gcgccagggt ccctatagtc ctccactgtt
     3241 tcgagggcgg cgaccgcgag gtgagggagg cgagggagcg cggctactac gttggacgcg
     3301 ctggcaacgt cacgtacgag aacagcgaga cggcgagggt gctccgcctc atagacgagg
     3361 aacgcatcct cgtcgagacg gacgcccctt accttagccc ccagccgttg cgcggcagga
     3421 agaacgcgcc caaaaacgtc gtccacacgg ccagcttcgt cgccgaaagg ctcgggctgg
     3481 aggacgagga gcttgctacg ctcaccaccc gcaacgcccg caacctcttc ggtctgccgc
     3541 tcgaggtgtg agcgtcggtg tccgagcagg gtccgccgaa gaagcgtctc ggccagcact
     3601 tcctgaagga cccgaacacc gccaggatag tcgcctcggg cgtgaagaag gatgacgtcg
     3661 ttctcgaggt cggtcccgga cgcgggttcc tgacggcgtt cctcgctgag aaggctcgtc
     3721 tggtacacgc cgtggaactc gaccccgacg tgctcccgag cctgcgtgag gccgtcgggg
     3781 acatggacaa cgtcctgatc cacgagggag acgccctccg cttcgattac tccaccctcg
     3841 accccgcacc gaacaagctc gtcgccaatc tgccttacaa catcgcttca ccgctcgtcc
     3901 taaggtttct tgaagaggta gatagtctca cgacgctgag gttcatggtg caactcgagg
     3961 tggcccgcag gatggcggcg gagcgcggga ccaaggacta cgcatcgtac gccgtgctgg
     4021 cgcaactgct ggcgagggtg aggatctcac acaaagtccc cccgacggtg ttcgacccgc
     4081 ctcccagggt acactcggcg gtcgtggaga tggagcgcag agaaccgccg gatgactacg
     4141 aaaggataaa gagcgtcgtg ctcggcgcgt tc
//
LOCUS       DATAIR010000680         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_254306, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000680 DATAIR010000000
VERSION     DATAIR010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254306"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..802)
                     /locus_tag="VHM69_16085"
     CDS             complement(179..802)
                     /locus_tag="VHM69_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741964.1"
                     /translation="MRRAVSLLGAAFALLFVLILFVLPAGAQNDGGIPPGTPANGSNP
                     DNPRGNPDIVTIASEGCRVAEGASVTLQDDENETRAIFTDNEKGIEIFDVDGRVRIKG
                     PDDDPSLSPHATFPDAGDTSFSTNGNYKVVSSTGITGCTGGGASPAQAQYEQYQKADG
                     VDSAKDVIPETIKVKKVPDTGGPPYIALGALVLLSVSVVVGGRILRP"
     gene            complement(864..2150)
                     /locus_tag="VHM69_16090"
     CDS             complement(864..2150)
                     /locus_tag="VHM69_16090"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX2741965.1"
                     /translation="MHDSARSNNETVFTMEATPLKYGPGASEEAGWELKRMGVGRVML
                     VSDPGIVKANITGRIVELIEAEGIEVEVWDRSRVEPTDESFQAAADFAAAGDFDGFVA
                     VGGGSSIDTAKVSDVIATHGGEILDYVNAPVGKGKKPPGPLKPLLAIPTTAGTGAEAT
                     TVAILDIPEQRVKSGISHQYLRPDRGVVDPLLTMTMPPEVTSSCGLDVVCHAAESYIS
                     KPYDARPKPQSPDDRPPYQGANPIADMWSAKALEYGGRYLRRAVKDGEDVEARGAMML
                     GASLAGIGFGSAGVHIPHACAYPIAGLKHEWQPPGYPEDHPFVPHGWSVIVTAPAAFR
                     FTYQSDPERHHTVAELLSGKEIVQADENTLPDIIVQLMKDVGAPRGVRELGYSEDDIE
                     DLVDGAMKQQRLLVGAPREVREDDLANIIRESMENW"
BASE COUNT          433 a          798 c          739 g          507 t
ORIGIN      
        1 acggctgtgg cctgcggcca accttcgtga gagctccgat cctccatccg ctaccaggtc
       61 tcctcaggtc ggcagagatc cagcgcttcc cgtgtatcca tatggttttt cgcccctcca
      121 ggttggctcg cttaccactt cggcacgaag ccatggtagc ttgggggcgg agcagacgct
      181 acggtctgag tatgcgcccc cccacgacca cagacacgga caggagtaca agggcgccca
      241 aggctatgta aggaggaccg cctgtgtcgg gcactttctt gactttgatc gtttcaggta
      301 tcacgtcttt ggcgctgtcc actccgtcgg ccttctgata ctgctcatat tgagcttgcg
      361 cgggacttgc gcctccaccc gtgcaaccgg tgatccccgt ggaggacacg accttataat
      421 tgccgttggt gctgaaagac gtatcaccgg catctgggaa cgtggcgtgt ggtgagaggg
      481 aggggtcgtc gtcaggacct tttatcctca cccgcccgtc cacgtcgaat atctcgattc
      541 ccttttcgtt gtcagtgaag atcgccctgg tctcattctc atcgtcttga agcgttaccg
      601 aggcgccctc cgcaacgcga cagccttccg aggcgatggt gacgatatcc ggattacccc
      661 tggggttgtc cgggttgctc ccgttcgcgg gcgtgccagg tggtatgcca ccatcattct
      721 gcgcaccggc tgggagcacg aagaggatca agacgaacaa cagggcgaag gccgccccca
      781 acaaagaaac cgctcgtctc atccccgccc tctttcgtca cacaaacgac gagaggtatt
      841 gtgcccgacc ggacagcgtt tcgctaccag ttctccatcg actcgcggat gatgttcgcc
      901 aggtcgtctt ctctcacctc acggggagcg ccgacgagca gccgttgctg cttcatcgca
      961 ccgtccacaa gatcctctat atcgtcttcg ctgtagccaa gctcgcgcac gccgcgcggg
     1021 gcgccgacat ctttcatcaa ctggacaatg atgtcgggca gcgtattctc gtcggcctgt
     1081 actatctcct tgccggagag cagctccgcg accgtgtggt gcctctcggg gtcggattgg
     1141 taggtgaacc ggaaggcggc cggtgccgtg acgatgacag accagccgtg tggcacgaaa
     1201 ggatggtcct ccgggtagcc aggcggttgc cactcgtgct tcaggccggc tatcgggtag
     1261 gcgcaggcgt gcggtatgtg gacccccgcg gacccgaagc cgatgcccgc gagagacgcc
     1321 ccgagcatca tagccccgcg cgcctcgacg tcctcgccgt ccttgacggc gcgcctcagg
     1381 tacctgcctc cgtactcgag tgccttcgcg ctccacatat ccgctatcgg gttcgccccc
     1441 tggtatggtg gcctgtcgtc cgggctctgc ggcttcgggc gggcgtcgta gggtttggag
     1501 atgtatgact cggcggcgtg gcagaccacg tcgaggccgc aggacgaggt gacctcgggc
     1561 ggcatcgtca ttgtgagcaa gggatctacg acgcctcggt cgggacgcag gtactggtgc
     1621 gagatgccgg atttcaccct ctgttcgggg atgtccagga tcgcgaccgt cgtcgcctcg
     1681 gcgccagtgc ctgccgtcgt ggggatggcg aggagcggct tcagcggtcc tggcggcttc
     1741 ttgcccttgc cgacgggggc gttgacgtag tccaggatct ccccgccatg cgtcgcaatg
     1801 acgtcggaga ccttcgccgt gtcgatgctc gacccgccgc cgaccgcgac gaacccgtcg
     1861 aaatcgccgg cagcagcgaa gtctgccgca gcctggaagg actcgtccgt cggctcgacg
     1921 cgcgagcggt cccacacctc gacctctata ccctcggcct cgatcaactc gacgatccgt
     1981 cccgttatgt tcgccttcac gatgccagga tcggagacca gcatcacccg cccgacgccc
     2041 atccgtttca actcccagcc ggcctcctcc gaggcgccgg gaccgtactt cagcggcgtg
     2101 gcctccatgg tgaagacagt ctcattgttg gatctggcgc tgtcatgcat gatagtcagt
     2161 ctcctttccc ccgcaagaaa taccctagcc tttggtaccc gcttcttccg gcaaatacac
     2221 gtgcgagcag gaccggcgac actcagaggc acccggtcga gaaccgcgag taatggttcc
     2281 ggtccgtcgt cgggtgtcta gtacatttgt cgtatgccac atagcataca aacgacacgc
     2341 aaaatatgcc gaacggcaga tgtgcggggt tacttcagat cctaaagttc ccactgtaga
     2401 accagcgact cgctggcttt atacggtaga ggctgagaaa cccgcccaaa tctctgggcg
     2461 cccggaacgg gtggagc
//
LOCUS       DATAIR010000681         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_346503, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000681 DATAIR010000000
VERSION     DATAIR010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346503"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VHM69_16095"
     CDS             complement(<1..905)
                     /locus_tag="VHM69_16095"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX2741966.1"
                     /translation="MEDTDILITGGYGTVGSRVAADLAPDYPGRLVLAGRSAAKAGQL
                     AAEIGYGVRGRRIDVGNPASVEAALGGVGVLVSCVDQPEPHLLRAAIARGLACTDIAP
                     HLMTRRPTEGMKAEATHTGARIVLGAGLAPGISSLLARMGADRVGAVDSIESNVLLSA
                     GDTYGPASRKYIMEEIDMAYAVRIEGREVLMRPFSRSARVNFPPPLGERTAYPFPFSD
                     QVFFPLTLGARTAITRLVLDPPWLGALLSALVRLRVMGVLRRQGRAQERGQQLIARLQ
                     RRYEGSDWYGVVVDIRGTRGRVRASL"
     gene            1352..1600
                     /locus_tag="VHM69_16100"
     CDS             1352..1600
                     /locus_tag="VHM69_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741967.1"
                     /translation="MSDGLKIVLGAVVGAFVVLLLVGGFSGGGMGYGMMGGMGQMMGG
                     YMMGGGILGMLLAGLFWILLLILLAAVVVWIFNQTQRH"
     gene            2026..2472
                     /locus_tag="VHM69_16105"
     CDS             2026..2472
                     /locus_tag="VHM69_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2741968.1"
                     /translation="MSPSKEFPPTTGALLRLAWQAVRERIYEGVLEAGYTDLSPAHVL
                     LFRWPTLDGLRPSELAARNQLSRQSINDLLGDLEKRGYLERTPDPTDGRARIVRLTEN
                     GWDLSQVLNDTGFATESEWARAIGEARFAEFRDTLRELVAYASPAG"
BASE COUNT          501 a          841 c          811 g          497 t
ORIGIN      
        1 agagaagccc gcacccggcc tcgcgttccc cgaatatcca cgacaacgcc gtaccagtcg
       61 ctcccctcat accggcgctg caatcgagcg atgagttgct gacctcgctc ttgagcacgt
      121 ccctgccgtc ggagcacccc cattacccga agccgcacga gggccgacag cagcgctcct
      181 agccaaggtg gatcgagtac caggcgcgtg atggccgttc gggcaccgag cgtcaaaggg
      241 aagaagacct ggtcggagaa cgggaacggg taggccgtgc gctctccgag aggcggaggg
      301 aaattgacgc gagcggacct ggagaacgga cgcatcagta cctcgcggcc ctcgatccgc
      361 accgcgtagg ccatgtcgat ctcctccatg atgtacttac gcgaggcggg cccgtaggta
      421 tctccggcgg agagcagcac gttgctctcg atgctatcga ccgcgccgac gcgatccgct
      481 cccatacggg cgagcaggct cgatatgccg ggagccagcc cggccccgag cacgattcgg
      541 gcgccggtgt gggtcgcttc ggccttcatg ccttcggtgg gacggcgggt catcaggtga
      601 ggtgcgatgt ccgtgcaggc caagccgcgc gctatcgccg cgcgtagcag gtgaggctca
      661 ggttggtcga cgcagctcac caagacgccc acgccgccga gggcagcctc aaccgaggca
      721 gggttgccaa cgtcgatcct tcgtccccga actccgtatc cgatctcggc cgcaagctgc
      781 ccagccttcg ctgcgctcct tcctgccaag acgagccgac ccgggtagtc aggtgcgaga
      841 tccgctgcga cgcggcttcc cacggtcccg tagcctccgg tgatcaggat gtcggtatct
      901 tccaatatgg gttcctcctc ctatcttccc acatgctcac acgtccttgc tgcgccagag
      961 cctgctcccc acgaggactt tgctcgagag cctggcgtac ccagagctga tacccgcctt
     1021 gtgagggctc gccgcatcgg ttggatcagc gcgtggcaag gggcgtaggc aaggtgcatg
     1081 ctgaacgcgc cgggaacgcg ttgcgcaccg ccgaaggggt catctacgct gtagtccgcg
     1141 ccgccttctt tgctgttacc ttctacgctc acgccgtggt accactcctc cgcaggcatc
     1201 ttgagggctc gaccgagccc catcgtgagt ccattcgtcc tgattgatca aagttgccca
     1261 acagaacggc acatattgct caggacaccg ccgccccccg gcgttagact cgaactgtgg
     1321 caagcagagt tcaatcgcaa ggaggtcgat aatgagcgac ggcttgaaga tagtacttgg
     1381 cgccgtggtc ggggccttcg tggtcctgct cttggtcggg ggcttctccg gcggcgggat
     1441 gggctatggg atgatgggcg gcatgggaca gatgatgggc ggctacatga tgggcggcgg
     1501 catcctgggg atgcttcttg ccgggctgtt ctggatcctg ctcctgatcc tgctggcggc
     1561 agtggtggtc tggatattca accagactca gcgccactag ccgccagccc atcagaccgt
     1621 agactggccg aaggcgcctg agggcattac atccccctca gcatctcggg atcgccggtc
     1681 tgcgttccgt caagcgtcca gttcagcacg ccttcgaacc cgtagatcgt ctcaccgtaa
     1741 ccatcgtggc tgtggacggt cggcatcctt gcccggctgg tacgtcgaac tcgaagaccg
     1801 ccacaaaccc ccccgactct tcggcttgga gcctgaatcg gatcgccatc tgcccgattc
     1861 ggatctcttc gcccgggctc aatagctatc tcccctgtct ccatttctct ctgttgtcca
     1921 tttagtacgc aattcgtatt aaaatgatac gaattacgta ctaaatgtca atagcgaaag
     1981 tgaaggatgc gcatctttca cgataagaaa gcgaggaggc aaactatgag cccgagcaaa
     2041 gaatttccgc ccactaccgg cgccctgttg cggctcgcct ggcaagccgt gcgcgagcgg
     2101 atctacgagg gtgttctcga ggcaggttac accgacctga gtcctgctca cgttctgctt
     2161 ttccgctggc cgacgctcga cgggcttaga ccatccgagc ttgcggcgcg caaccagctt
     2221 agcaggcagt ccatcaacga cttgcttggc gatctggaga agaggggcta tctggagcgc
     2281 acccctgacc ccacggacgg acgcgcccgg atcgtacgcc ttacggagaa cgggtgggat
     2341 ctatcgcagg tgctgaacga cacgggattt gccacagaga gcgagtgggc acgggcgatt
     2401 ggagaggcac gcttcgccga gttccgtgac acgctgcgcg agttggtggc ctacgcctcg
     2461 cccgccgggt aggctgctac aggaggtagc gaagggcagc actggcgaac ttcgtaggaa
     2521 gctgaagagc gcgatgacag gtaccagctg acgtcgcttc gtggagaccc acaacagcct
     2581 gctgctgcgc cgctagtaat ggtgagacaa agcacaccaa aaggctgagc ccgaaagccc
     2641 cgccccaact
//
LOCUS       DATAIR010000682         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_161577, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000682 DATAIR010000000
VERSION     DATAIR010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161577"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1161)
                     /locus_tag="VHM69_16110"
     CDS             complement(226..1161)
                     /locus_tag="VHM69_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HEX2741969.1"
                     /translation="MTRIFDAGETASRLPYPALADSIREAALAMSSGELQAPPRLVLS
                     LSEGGVLLVMPASDQEISITKLVTVHPENAARGLPTIQGEVVVMKAATGERLGILEGS
                     VVTARRTAALSMLAARELAPRPDGPLLVVGAGTQGRSHLEAFHEGLGVSKVFISSRTA
                     DSATSLAEYARDLGIDAEAVERPKEVLDEVSLVVTATTSREPVLPDEIPHGTFVAAVG
                     SFEPEAAELPPALLSTSKVFVDTMEGAKEEAGDLIQAVQAGDFRWEDATALEDALRSS
                     GRPEGTVVFKSVGHALWDLAAARTAFVDQPGRDTS"
     gene            complement(1158..1661)
                     /locus_tag="VHM69_16115"
     CDS             complement(1158..1661)
                     /locus_tag="VHM69_16115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2741970.1"
                     /translation="MKVRREAERDIPAIREVNRGAFDREVEARLVDDLRSEGYAKVSL
                     VAEEGGRIVGHIMFSEAVIRTGSGEVAALALGPVAVTPERQGQGIGSALVREGLDQCP
                     QTLYQSVFLLGIPGYYPRFGFSADLAGSFSSTYSGEAFMALELVPGALAEITGEFEFA
                     PPFEAVS"
     gene            complement(1658..>2706)
                     /gene="solA"
                     /locus_tag="VHM69_16120"
     CDS             complement(1658..>2706)
                     /gene="solA"
                     /locus_tag="VHM69_16120"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX2741971.1"
                     /translation="GLERHTPAHDRGSSHGESRIIRQAYFEDPAYVPLLLRAYELWER
                     LEEETGEDLMTLCGGLMIGPRDGVLVSGSVASAQEYSLPYEILDSSELKRRYPIFEPG
                     PETIALFEEKAGFVRPEASVKAHLDRAASLGADLRFEEEILSWEPRESGVRVETASGT
                     HEAERLVVSAGAWAPKLLADLGLPLEVTRQILFWFRPEGGTESFAPEKVPIWIYEPDD
                     GNMFYSFPAIDGADTVKVAFFRVDGKPADPETIDREVHDDEVEFIRGYLAKYVPSLNG
                     DFHFAKTCMYTNTPDEHFVISTHPEYPQVSIAAGFSGHGYKFCSVVGEILADLVTEGK
                     TSHPIDLFSPERLW"
BASE COUNT          481 a          880 c          866 g          479 t
ORIGIN      
        1 cctgaacctc ctgctcctgg gagtctgtga ggacgccggc ctcgtcgtag acggtgtcag
       61 tgccctgggc cagggcgacc gagtaggcac ccgccagcat ggccagcgag atcaagcaca
      121 acgcgatgag tctcgaccct attgtctgct ccagtcgtgt cgatcgtaca ttgctacgac
      181 accggaacca aaaggtttct cgcagggatc gttcttagat cttctctacg acgtatctct
      241 tcctggctgg tctacgaaag ccgttctggc ggcggcgagg tcccacagcg catggcccac
      301 gctcttgaat acgaccgtcc cttccggacg ccctgacgag cggagcgcgt cctccagcgc
      361 cgtcgcgtcc tcccaccgga agtctccggc ctgcacggcc tggattaggt ccccggcctc
      421 ctccttcgcg ccctccatcg tgtccacgaa gaccttcgag gtagagagca gagccggcgg
      481 caactccgcc gcctcgggct cgaaagaccc gaccgccgcc acgaaggtgc catgggggat
      541 ctcatcaggg agcacaggct cccgactcgt ggtggcggtg acgacgaggc tcacctcgtc
      601 cagcacctct ttgggacgct cgacggcttc ggcatctatg cccagatcac gggcgtactc
      661 ggcgagggat gtggcgctat ccgcggtccg tgaggagatg aagaccttgg agacgccgag
      721 gccctcgtgg aaggcttcga ggtgcgagcg tccctgcgtt cccgcgccca cgacgaggag
      781 cggtccgtcg ggacgcgggg cgagctcgcg ggctgcgagc atcgagagcg cggcggtgcg
      841 cctggcggtg actacgctgc cttcgaggat gccgagcctt tcacccgtcg ccgccttcat
      901 cacgacgacc tcgccctgta tggtcggtag gccgcgggca gcgttttcag ggtggacggt
      961 gacgagcttg gtgatggaga tctcctggtc cgaggccggc atgacgagca ggacacctcc
     1021 ctcagagaga gacaggacga gccggggagg cgcctgcaat tcgccggagc tcatggcgag
     1081 cgcggcctcg cgaatagagt ccgcaagcgc agggtagggc aggcgggatg cggtctcccc
     1141 agcatcgaag atacgggtca cgacaccgcc tcgaacgggg gggcgaactc gaactctccc
     1201 gtaatttccg cgagggctcc gggtacgagt tccagcgcca tgaaggcttc cccggagtag
     1261 gtggaggaaa agctcccggc cagatccgcc gagaagccga aacgtgggta gtagcctggg
     1321 ataccgagca ggaagacgct ttggtagaga gtctgtgggc attggtcgag gccctccctg
     1381 acgagcgcgg agcctatacc ctgcccctgg cgctcagggg taacggccac aggtccgagc
     1441 gccagggccg ccacctcgcc gcttcccgtc cggatcacgg cttcgctgaa catgatgtga
     1501 ccgacgatcc ggccaccttc ctccgcgacc agagatactt tagcgtatcc ctcgctacgc
     1561 agatcgtcga cgagacgggc ctcgacctcc ctgtcgaatg ccccgcggtt cacctccctg
     1621 atagccggga tgtccctctc agcctcgcgc cggaccttca ccacaaacgc tccggggaga
     1681 acagatctat agggtgggaa gttttgcctt ccgtgacgag gtcggccagg atctcaccta
     1741 ccacgctgca gaacttgtag ccgtgccccg agaaaccggc tgcgatggag acctgggggt
     1801 attcggggtg ggtggatatg acgaagtgct cgtcaggggt gttcgtgtac atgcaggtct
     1861 tcgcgaagtg gaagtccccg ttcagcgagg ggacgtactt cgccaggtaa ccccggatga
     1921 actctacctc gtcgtcgtgg acctctcggt ctatcgtctc ggggtccgcc ggtttcccgt
     1981 ctaccctgaa gaacgcgacc ttgaccgtat ccgcaccgtc gatggcagga aagctgtaga
     2041 acatgttgcc gtcgtcgggc tcgtagatcc agatagggac cttctcgggc gcgaaggact
     2101 ctgttccccc ttcgggacgg aaccagaaaa ggatctgacg cgtaacctcc agagggagac
     2161 cgaggtcggc aaggagctta ggcgcccacg cgcccgccga gactaccaga cgctccgcct
     2221 cgtgggtccc tgatgccgtc tcgacacgta cacccgactc ccttggttcc caggacagta
     2281 tctcctcttc gaaacggagg tccgcgccta aagaggcggc cctgtccagg tgggccttca
     2341 cggaagcctc gggcctgacg aagccggctt tctcctcgaa gagggcgatc gtctcgggac
     2401 caggctcgaa gatcgggtag cggcgcttca gctcggagga atccaggatc tcgtacggca
     2461 agctgtactc ctgtgcgctc gcgacgctgc ccgatacgag cacgccgtcg cggggaccga
     2521 tcatgagccc cccgcagagc gtcatgaggt cctcgcccgt ctcctcctcc aacctctccc
     2581 acagctcgta ggcacgcagg agcagcggca cgtaggcagg gtcctcgaag tacgcctggc
     2641 ggatgatcct ggactctccg tggctggatc ccctgtcgtg ggccggggtg tggcgctcca
     2701 gcccga
//
LOCUS       DATAIR010000683         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_161623, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000683 DATAIR010000000
VERSION     DATAIR010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161623"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VHM69_16125"
     CDS             complement(<1..1825)
                     /locus_tag="VHM69_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX2741972.1"
                     /translation="MTGGKAVNERLWDFREVGRLPAPGDNVAMATRRLEAGTRVSYEG
                     SEFTVGHTVLEGHRFAVEPVAEGEDLLSWGLRFGRAIRDIVPGDYACNEKILRVLRER
                     FRASYRGDEDPEGTSDQGGGRVPGGYSKAGLILPEEPNFRDAELEPYLFDEESFRAGE
                     QVPLNDEVRTFMGYRRGADRGVGTRNYVVVMGVTSRLTGFVRALELEMNGVAESYDNI
                     DGIVCVAHTEGGEERAPNNLDLLLRTLSGFMVNPNVGAVLVLDHGGEEAVTNRMLQAY
                     VEEHGYPVHDVPHEFISLEGGFRRDLERAKSVVRGWLEEVNAARRTEVPASELKIGLQ
                     CGGSDAFSGVSANPLVAWVSREVVRNGGIANLAETDELIGAEHYVLKYVSDLQTAKRF
                     LSTVERFKERVSWHGHTAEDNPSGGNNYRGLYNISVKSIGAAMKKHPDVRIDRVIEYA
                     ERMDEGGFYFMDSPGNDLESVAGQVASGANMIFFTTGNGSITNFPFVPTIKFVTTTGR
                     YELLSKDMDVNAGAYLDGTPMEDLGRETFERTLRAASGERTVGERAGHSQVSIWRDWK
                     QTSDENLALLGNAPEPDGEPLPIKKDIPDVEFSFEAIRSGRG"
     gene            complement(1898..>2152)
                     /locus_tag="VHM69_16130"
     CDS             complement(1898..>2152)
                     /locus_tag="VHM69_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741973.1"
                     /translation="AGWFGETARLRFAPWEEWREGVSKEEAEGTWDHIAHSPNCSIAK
                     ARRLLDYQPRYSSLQAVRESLGWLIEHNKLEAEWTIGHST"
BASE COUNT          334 a          731 c          650 g          437 t
ORIGIN      
        1 gcccgcgccc gcttcttata gcctcgaatg agaactcgac gtcaggaatg tccttcttta
       61 tgggcaaggg ctcgccatcg ggctcgggtg cgttcccgag caacgccagg ttctcgtcgc
      121 tcgtctgctt ccagtcgcgc cagattgaga cctgggagtg gcccgcgcgc tccccgacgg
      181 tcctctcgcc cgaagccgcc ctgagcgtcc gttcgaaagt ctcgcggcca aggtcttcca
      241 tcggggtgcc gtcgaggtag gcgccggcat tcacgtccat gtccttggag agcagttcgt
      301 agcggcccgt ggtggttacg aactttatgg tcgggacgaa ggggaagttg gtgatggagc
      361 cgttgcccgt ggtgaagaag atcatattcg caccagaggc tacttgcccg gcgacgctct
      421 cgaggtcgtt gccggggctg tccatgaagt agaagccgcc ctcgtccatc ctctcggcgt
      481 actcgatgac gcggtcgatg cgcacgtcag gatgcttttt catcgcagcc ccgatggact
      541 tgacggagat gttgtagagc ccgcggtagt tgttccctcc tgacggattg tcctcggccg
      601 tgtgtccgtg ccacgagacg cgctccttga accgctcgac cgtggagagg aaccgtttcg
      661 ccgtctggag gtcactgacg tacttcagga cgtagtgttc ggccccgatc aactcgtccg
      721 tctcggccag gtttgcgatg ccgccgttcc tgactacctc cctgctcacc cacgccacga
      781 gcgggttcgc cgagaccccg gagaaggcgt cggacccgcc gcactgcaac ccgatcttca
      841 actcggacgc aggcacttcg gtgcgtcgcg cggcgttcac ctcctcgagc cagccgcgca
      901 cgacggactt ggcccgttcg aggtcccgcc tgaaacctcc ctccaggctg atgaactcgt
      961 gcggaacgtc gtgaacggga tagccgtgct cctccacgta cgcctggagc atcctgttcg
     1021 tcacggcctc ctcaccgccg tggtcgagga ctagcacagc ccctacgttc gggttcacca
     1081 tgaaaccgct gagggtcctt aggagcaaat ccaggttatt aggcgcgcgc tcctccccgc
     1141 cctccgtgtg ggcgacgcag actatgccgt ctatgttgtc gtaggattcg gcgacgccgt
     1201 tcatctccag ctcgagcgca cgcacgaagc cggtgagcct agacgtgacg cccatcacga
     1261 ccacgtagtt gcgcgtcccg acgcccctgt ccgccccgcg cctgtagccc ataaaggtgc
     1321 gcacctcgtc gttgaggggg acctgctctc ctgcccggaa gctctcttcg tcgaagagat
     1381 agggctccag ctccgcgtcg cggaagttcg gttcttcagg cagaatcagg ccggccttgc
     1441 tgtaccctcc agggacgcgt cctccgccct ggtccgaggt cccttcagga tcttcgtccc
     1501 cccgatacga agccctgaag cgttcgcgca gcacgcgcag gatcttctcg ttgcacgcgt
     1561 agtctccggg cacgatgtcc ctgatggcgc gcccgaagcg cagtccccag gagagaaggt
     1621 cctccccttc tgccacgggc tcgacggcga agcggtgacc ttcgagcacc gtatgcccga
     1681 ccgtgaactc cgagccttcg taggatacgc gcgtcccggc ctccagcctg cgggtcgcca
     1741 tagccacgtt gtcgccgggc gccggcaggc gtccgacctc ccgaaagtcc cagagccgtt
     1801 cgttcacagc cttgcctccc gtcatcttct cagccgatct cgcggtgagt gtatcgtgcc
     1861 ctgccgcccg gggtggtagc tcgcgagagt ctgacttcta tgtggagtgc ccgatcgtcc
     1921 attccgcctc tagtttgttg tgttcgatca gccatccgag agattcacgc acggcctgca
     1981 gcgagctgta acggggctgg tagtcaagca agcgcctggc tttggcgatg ctgcagttgg
     2041 ggctgtgggc gatgtggtcc caggtcccct cggcttcctc cttcgataca ccttcgcgcc
     2101 actcctccca gggcgcgaag cggaggcggg ccgtctctcc gaaccagccg gc
//
LOCUS       DATAIR010000684         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_255277, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000684 DATAIR010000000
VERSION     DATAIR010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255277"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VHM69_16135"
     CDS             complement(<1..741)
                     /locus_tag="VHM69_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2741974.1"
                     /translation="MKGTSFHTQVLAIARRSTLRTMRQPAVIVSALLFPMMFFSINAS
                     GLDAASRIPGFPTDSYLDFAFAFPLIQASLFGAVTAGSDLARDIESGFFDRLSLTPLR
                     PVALLGGMLAGVVALGLLQGVVFLTIGLLMGVNVSSGFFGMIVIVLLTVLVALGFGGI
                     GAMLAIRTGSVEAVESAFPLFFVAIFLSSINLPRDLIEQDWFRFIATINPISYLVEGI
                     RSLVITGWDAQALILGFGFAIAIVVVAIG"
     gene            complement(738..>1731)
                     /locus_tag="VHM69_16140"
     CDS             complement(738..>1731)
                     /locus_tag="VHM69_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2741975.1"
                     /translation="GRLVGRERGIEVRNLVREFRKGPRAVDGIDLRVEPGEIYGFLGP
                     NGAGKSTTVLMLTTLLPPTSGTATVAGYDIVSEGPQVRASIGAALQEAALDPFLTGRE
                     HLHLQGALHGLSRKERRRRGRELLERVGLEEAADRKVRGYSGGMKRRLDLALALVHGP
                     QILFLDEPTTGLDPQSRSALWAEVSRLAREDGVTVFLTTQYLEEADVLADRVGIIDRG
                     RIVAEDTPEALKAEIGRPSVEATPAERRERDAVARVLGQFGEEIPAQPGAVAVRLSAG
                     TAGLADVVRALDRENLHVANLRLDEPSLNDVFLAKTGRSLEGAAEDNEMEEAQA"
BASE COUNT          310 a          589 c          565 g          267 t
ORIGIN      
        1 gccgatcgcg acaacgacga tggcaatggc aaagccgaaa ccgaggatca gagcctgcgc
       61 gtcccagccg gtgatcacca ggctccggat gccttcgacc aggtaggaga tcgggtttat
      121 ggtcgcgatg aatcggaacc agtcctgctc gatcaggtcc cgcggcaggt tgatggaaga
      181 caggaagatg gccacgaaga atagcgggaa cgcgctctcg acggcctcga ccgaacccgt
      241 ccgtatcgcc agcatcgccc ctatcccccc gaagccaaga gcaacgagca ccgtcagaag
      301 cacgatcact atcatgccga agaatccact gctcacgtta acgcccatga gcagcccgat
      361 ggtgaggaag acgacaccct gaagcaggcc gagcgcgacc accccggcga gcataccgcc
      421 gagcaatgcg acggggcgca gcggggtgag cgagagcctg tcgaagaagc cgctctctat
      481 gtcgcgggcg aggtcggagc cggccgtgac ggcgccgaag agcgaagcct ggatgagcgg
      541 gaacgcgaag gcgaagtcga ggtacgagtc ggtcgggaac cccgggatgc gagaggcggc
      601 gtcgaggccg ctagcgttta tggagaagaa catcatcggg aagagcagcg cggagacgat
      661 gacagcgggc tgcctcatgg tccgcaacgt ggagcggcgt gcgatcgcca ggacttgcgt
      721 gtggaacgaa gtgcccttca cgcctgcgcc tcttccatct cgttgtcctc cgccgcgccc
      781 tccagcgagc ggcccgtctt ggcgaggaag acgtcgttga ggctgggctc gtcgaggcgg
      841 aggtttgcga cgtgcaaatt ctcccgatcc agagcacgca ccacgtcggc caggccggcg
      901 gtgcctgcgg agagtctcac ggcgacggct cctggttggg ccgggatctc ctccccgaac
      961 tgtcccagga cgcgcgcgac ggcgtcgcgc tcccggcgct cggccggcgt agcctctacg
     1021 ctggggcggc cgatctcagc cttcaaagcc tccggcgtgt cctcggcgac gatcctgcca
     1081 cggtcgatga tgccgaccct gtccgccagc acgtcggcct cttcaagata ttgcgtcgtc
     1141 aggaacaccg tcacgccgtc ttcgcgcgcg agcctgctga cctcagccca caaagcgctg
     1201 cggctctggg ggtcgaggcc cgtcgtaggc tcgtccaaaa agagtatctg cgggccgtgc
     1261 accagcgcga gtgcgaggtc gagcctgcgc ttcataccgc cggagtagcc acgcaccttc
     1321 ctatcggcgg cctcttcgag cccgacgcgc tcgagtagct cccgtccccg ccgcctgcgc
     1381 tccttacgcg agagcccgtg cagggctcct tgcaggtgca ggtgctcgcg gcccgtcagg
     1441 aatgggtcga gcgccgcctc ctgcagcgcc gccccgatgg aggcccgcac ctgcggcccc
     1501 tcgctgacga tgtcgtagcc tgccaccgtg gccgtgccgc tcgtgggcgg aagcaatgtc
     1561 gtcagcatca gaaccgtcgt cgacttgccg gcgccgttcg ggccgaggaa gccgtagatc
     1621 tcaccaggct cgacgcgcag gtctatgccg tcgacggccc gcggtccctt cctgaactcc
     1681 ctgacaaggt tcctgacctc tatcccccgc tccctgccca ctaggcggcc a
//
LOCUS       DATAIR010000685         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_255743, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000685 DATAIR010000000
VERSION     DATAIR010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255743"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VHM69_16145"
     CDS             complement(<1..615)
                     /locus_tag="VHM69_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2741976.1"
                     /translation="MGKQKLDRPGGTIDFGSGNGEVGTGRRARRGVPAVHAEGLVYRF
                     GDNVAVAGVDLEISPGEIFGFLGPNGAGKTTTIRMLATLLRPHSGTARVFGHDVVRES
                     DAVRGRVSLTGQFASVDDDLTGYENLVLVARLLGYSRKGAKGRAGELLDAFGLSEAAG
                     RLVKTYSGGMRRRLDIAASIVVTPDLLFLDEPTTGLDPRNRNQVW"
     gene            916..2028
                     /locus_tag="VHM69_16150"
     CDS             916..2028
                     /locus_tag="VHM69_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2741977.1"
                     /translation="MRKLSRGSSENGREELLGELVGEVRRLNGLGASFLRAVAGRVGM
                     NATDLQVVDILGSAGPITAGRIAELTGLTTGATAQMLGRLERDGIVRRERDPDDGRRV
                     LVRLAAGWDAMEKIGPTFDSAGRAWEELASRYDQGQLALILGFLQRANSVYEEEIYRL
                     REAPPEGRDFSAPLQGVESGRLVFSSSASRIVLRADARMDELYRASFEGPVPEVKAEG
                     GTVTFRYPRRLRLFDRSKQGAEVALNVAVPWRIEIRGGASEVVAELGGLDLSGLEVRG
                     GASSFRVELPEPNGTVPVRIAGGASEIVVRRPPHVAARVRLKGWASQLTFDDQTFGAV
                     GSDVRLQSPGYEDAAGRYDVEVSGSASDFTLTTTPG"
     gene            complement(2630..3991)
                     /locus_tag="VHM69_16155"
     CDS             complement(2630..3991)
                     /locus_tag="VHM69_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741978.1"
                     /translation="MRSESFAAVASYQRLRLREIRGLYEELDPSLEHYLGVERGISDE
                     IDSLEDEFAQTRKTLLSLGADLRRLAPVIDPENSMAPAMPQHRRRETGSADDFRNLVR
                     RAEGYLEDAGLDPNRDPLLQVLDPLEVAGIESRYRSEFGDVAWDESDYLVVIFAGFVA
                     TLLDVFLVRIPMDGAFLGKLQNGSPLTKWIKENSTTVHRDYFGHLEKVAKVPYDLSIG
                     KAVDGLRPKVHRLMSPGHDPVLGFVFGVKDIMDGTGTYIDKNGDLLRIGTSMSPEDLN
                     VAFLKVFLHLVSDVGTSAGIPPPFFTLLQLDKAKSPFVLGPSGERVSWTDVARYMYAH
                     GYDLRHFATMGIVPASVEMIVRGWWLCRRCESGEEPELAKAKLTSMLLLGHTIAASGN
                     LLKTGVIFGMNPLALNWAQMLALFPAAMAWVKESLKRDREIRAALDEQWLSMYRADLG
                     YSP"
     gene            complement(3993..4919)
                     /locus_tag="VHM69_16160"
     CDS             complement(3993..4919)
                     /locus_tag="VHM69_16160"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX2741979.1"
                     /translation="MTLEARPEAVKVLYARVLAAQCLVDGRLDPREIQYLYVFMSRIG
                     LGPESREEVRRSLDAGEVSSEVLVRLVEEVISQVPDNEEEIGVSVIKDMVQVSRADGV
                     VQPPEEETITVVAEARFGERAEQVIELAGKTVEYEQALIKGDVSASELEGHIKEIAAV
                     ATAASVPITAIFFSGSVVGLSAAGITSGLAALGLGGLLGLSAMVTGIGTVVVLGVAAY
                     AGTRWLLGGRERELAKQREHMIQEVIKRHEGAIEDLAEDVTGIGLKLAEYVSRSDQNE
                     ARLERLKDDLRMFNAALAELRQEKGNMEARSA"
BASE COUNT         1038 a         1872 c         1768 g         1040 t
ORIGIN      
        1 ccagacctgg ttgcggttcc tggggtcgag gccggtggtc ggctcgtcga ggaagaggag
       61 gtccggcgtg accactatcg acgccgcgat gtcgagcctg cggcgcatgc cacccgagta
      121 tgtcttcaca agccggcccg ccgcctccga gagcccgaaa gcatcgagga gttcaccggc
      181 gcgccctttc gctcccttgc gggagtagcc gaggaggcgg gccacgagga cgaggttctc
      241 gtagccggtg aggtcatcgt ccaccgaggc gaactgccct gtaaggctca cccgaccgcg
      301 caccgcgtcg gattcccgaa cgacgtcgtg gccgaacacc cgcgccgtgc cggaatgcgg
      361 gcgtagcagg gtcgcgagca tccgaatcgt cgtcgtcttg cccgcgccgt tcggaccaag
      421 gaagccgaag atctcccccg gcgagatctc gaggtccacg cccgcgacgg ccacgttgtc
      481 cccgaagcgg tagacgagcc cctccgcgtg caccgcgggc accccacgcc tcgccctccg
      541 tccggtgcca acttccccgt tgccggaacc gaagtctatc gttccgcccg gacggtccag
      601 tttctgtttg cccatctcca acctcctctg acgattcacg agtcaacgac tcggacgacg
      661 tgagtccacg cgggctgcta tcctcggagg cgcaggcccg gaggcaggtc tccccgcgag
      721 gaccttctct ggtgctcacg acccccggtg cggcggtgcg ccaacatccc gccgggggtc
      781 ctccttaatt cccctgatac aaccctagat cctctcctcc ttccaaacca ccgtgctata
      841 ctcgttttgc tagctaacga agccatatta ctatataata acttcgtagc gaagctaaat
      901 gaaggatact cgggggtgag aaagttgtca aggggttctt cggagaacgg gcgcgaggag
      961 ttgctggggg agctcgttgg cgaggtccgg cggttgaacg ggctcggcgc gtccttctta
     1021 cgggcggtgg ctgggcgggt tgggatgaac gccacggacc tgcaggtcgt tgacatcctg
     1081 ggcagcgcgg ggcctataac cgcgggacgg atcgccgagt tgaccggcct cacgacgggg
     1141 gcgactgcgc agatgctggg caggctcgag agggatggga tcgtgcgccg ggagagggac
     1201 cccgacgacg gccgcagggt actggtccgc ctcgccgcgg gctgggacgc gatggagaaa
     1261 atcggcccca ccttcgattc cgcgggcagg gcgtgggagg agttggcctc gcgctacgac
     1321 caagggcagc tagccttgat cctggggttc ctccagcgcg ccaactcggt gtatgaggag
     1381 gagatctacc gtctgcggga ggcgccaccg gaggggaggg acttctccgc gcccctgcaa
     1441 ggcgtggaga gtggcaggct cgtcttctcc tcgtcggcct cccggatcgt gttgcgcgcg
     1501 gacgcccgga tggacgaact ctaccgggcc agcttcgagg ggcccgtgcc ggaggtgaag
     1561 gctgaggggg gaaccgtcac cttccgctac ccgcggcgcc tgcggctctt cgaccggagc
     1621 aagcaggggg cggaggtcgc gctgaacgtc gccgtcccct ggcgcattga gatcaggggc
     1681 ggggcctctg aggtcgtcgc ggagctgggg ggcctcgacc tctcagggct ggaggtcagg
     1741 ggaggggcga gctcgttcag ggtggagctc cccgagccca acggcacggt ccccgtgcgg
     1801 atcgccggcg gagcgtcgga gatcgtcgtc cggcgccccc cgcacgtcgc cgccagggtc
     1861 cgcctcaagg gctgggcctc ccaactaacc ttcgacgatc agaccttcgg cgccgtgggc
     1921 agcgacgtga ggctgcagag tcccggctac gaggacgccg caggacgcta cgacgtagag
     1981 gtctccggca gcgccagcga cttcaccctg acgacgaccc ccggctgacg aagagccgcg
     2041 tcgagccgcg aggagcgcgt cgagatcccg aagtgaaaga atatcgcggc gacagtcccg
     2101 ccgtccgagt cctgatggac cctacaccac cgtggccgcc ctgttggctc acaggacccg
     2161 ccagaacgaa gagaggctgg cgtcccccgg gtggcgcgac cacgacctcg tcttccccaa
     2221 ccgcctcggc ggtccgatgg ctcacaacaa ctgctactcc cgcgagtata aaccgctaca
     2281 gaagcggtcg ggccacgccg acgaaggctt cacattacac gccctacgcc acaccttcgc
     2341 gaccgcgctc ttcaagcggg gcgaacaccc taagatcgtg cagtccttgc tcggacacgt
     2401 ctcgatcacg cagacgatgg cacctactcg cacctcatgg atggcatggg caacgacgcg
     2461 gtatacgggc tggacgaggc tttcggctag cctccgttcg gacaagttct tttcgcagcg
     2521 ggcagtccga aactgggtgg atttcaacca atgcgtggtc gattttcagc cacattgggg
     2581 gatcccggca gtacggtgag cagaatgccg ctgtgtggca ggaccggtgt catggagagt
     2641 agcccaggtc ggccctgtac atgctcagcc actgctcgtc taaggctgct cttatctccc
     2701 gatccctctt cagactctcc ttcacccaag ccatcgccgc gggaaagagc gcgagcattt
     2761 gcgcccagtt cagggcgagc gggttcatac cgaaaatcac ccccgtcttg agaaggttcc
     2821 cggaggcggc gatcgtatgg cccagcaaca acatggaggt cagcttcgcc ttcgcgagct
     2881 ccggctcctc cccactctcg catctccggc acagccacca cccgcgaacg atcatctcga
     2941 cggaggcggg cactatcccc atcgtggcga agtgccgcag gtcgtagccg tgggcgtaca
     3001 tataccgggc cacgtcagtc cacgataccc tctcccccga cgggcccagg acgaagggcg
     3061 acttcgcctt gtcgagctgc agcaaggtga agaaaggtgg cggaatccct gcgctcgtgc
     3121 ccacgtccga gacaaggtgg agaaagacct tcaggaaggc cacgttcagg tcctccgggc
     3181 tcatcgaagt cccgatcctg agcaggtcac cgttcttatc tatgtacgta cccgtgccgt
     3241 ccatgatgtc cttgaccccg aacacgaaac cgaggacggg gtcgtgcccg ggactcatca
     3301 gccggtggac cttgggcctc aaaccgtcca cagccttccc tatcgacagg tcgtagggca
     3361 ccttcgctac cttctcgaga tgtccgaagt aatctcggtg gacagtcgtg gagttctctt
     3421 tgatccactt cgtgagggga gaaccgttct gcagcttgcc caggaaggcg ccgtccatcg
     3481 gtatccttac caggaacaca tcgaggaggg ttgcaacgaa gccggcgaag ataacgacca
     3541 ggtaatcgga ttcgtcccag gcgacgtcgc cgaactcgct cctgtagcga ctctcgatcc
     3601 cggcgacctc gagggggtcc aggacctgga gcagcggatc cctgttcggg tccaacccag
     3661 catcctcgag gtatccctcg gccctcctca ccagattgcg gaagtcgtcg gcgcttccgg
     3721 tctcgcgtcg ccggtgctgc ggcatcgcgg gagccattga gttttcggga tcgatgacag
     3781 gcgccagcct gcgcaaatcg gcacccaaag agagcaaagt cttcctggtc tgggcgaact
     3841 cgtcctccag ggagtcgatc tcatccgaaa taccgcgctc gacgccaagg tagtgctcca
     3901 agcttgggtc cagctcctcg tagaggcccc ggatctcccg tagccgtagc cgctggtagg
     3961 atgcgaccgc cgcgaaggat tcagaccgca tactaggcgc tcctcgcctc catgttcccc
     4021 ttctcctggc gtagctccgc caaggcagcg ttgaacatcc gaagatcgtc cttcaggcgt
     4081 tctagacgcg cctcgttctg gtcggatctg gaaacgtact ccgccagctt caggccgatg
     4141 ccggtgacgt cttctgccag gtcttctatg gccccctcgt ggcgcttgat gacctcctgg
     4201 atcatgtgct ctctctgctt cgcaagctcc ctctcccttc cgcctaacag ccacctcgtc
     4261 cccgcgtaag ccgccacccc cagtacgacc acggtgccta tcccggtaac catcgcgctc
     4321 aatcccaaga ggccaccgag tcccagcgcc gcgagcccgg aagttattcc cgcggcgctc
     4381 aggccgacaa cacttccgct gaagaagatc gccgttatcg ggacgctcgc ggcggtcgcg
     4441 accgcggcta tctcctttat gtgtccttcg agctcgcttg cgctgacgtc gcccttgatc
     4501 agggcttgct cgtactccac ggtcttgccg gccagctcga tcacctgctc ggccctctcc
     4561 ccgaaccgcg cttccgcgac caccgtaata gtctcctcct ccgggggctg caccaccccg
     4621 tcggcacgcg agacctgcac catgtccttg atcacggaca cgccgatctc ctcctcgttg
     4681 tcggggactt gggagatgac ctcctccacc aggcggacca gaacttcgga actgacctcg
     4741 cccgcgtcca acgaacgcct aacctcttcg cgcgattcgg gaccgaggcc gatccgggac
     4801 atgaacacgt agagatactg gatctcacgc gggtcgagcc tcccatcgac gaggcactgg
     4861 gcggccagga cccgagcgta gagtaccttc accgcctccg gcctcgcctc aagggtcaca
     4921 ccagtccaca cggtcagcgc atgagctcgg gcaagcctgc gcggatctgc cagatcttgg
     4981 caatcctcat tacgtagatt tggatgtgtg gtcagtagtt cgctcatctc cgtaaggtag
     5041 cgttgtagtt cgtcaacctg agctcgtgat gggtggaacc tgaccccagg gccccagtga
     5101 gaggttgcga acgcggcggc gcgatgagcg gccaggagag ccgcgctctt ctgcccccat
     5161 gctacgctcg gccacgacgc ctcgtgttac cgggaggtaa accgcgcgtt aagacctcgt
     5221 gcaacgcgaa ggagtgccag ggtgggtatg ttgccaggat cccgtcccag aagatgatgg
     5281 tagagcagac ggccctataa gggagtttgg ctgcagtcca ttcgcgcttt cttgctggac
     5341 ggtgcaagag cgaggtaccc taacgcagga ggacaccctg tgatcaagga accccacggt
     5401 tccatcgccg cacctttgtt gatggcggcc ttaccagaga gtaggatggt atttatgatc
     5461 cgtgatccca gggacgtggt ggcgtcccgt ttggatgccc ataagatggg cggctggacc
     5521 cgcaacctga cgggtaaatc tgggagaagg ctcgcagata cagacgcatt cgtgagaacg
     5581 gcagctaatg cgtatctacg ggagtggagt tttacgacaa gcgaacgggg tcgtatcggc
     5641 tagtgttggc gtacgcttgg atagaggcct tcaaatcgat tctacgttca ccggctatcg
     5701 tggcttcagc ataaatgc
//
LOCUS       DATAIR010000686         6576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_210338, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000686 DATAIR010000000
VERSION     DATAIR010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210338"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VHM69_16165"
     CDS             complement(<1..870)
                     /locus_tag="VHM69_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741980.1"
                     /translation="MTEGAREQGRGVDRTSGARWLAWLLWTLTVVCALSLLVVREQGS
                     LIAVLPVAVWVVTSTTVGALIVSRRPDNSIGWILCASGFLFAFSIFSGMYAIYALVTY
                     PGSLPAGEVAAWFAAWVQEPVYSLFVFLFLLFPDGRPLTRRWRYVMWINGILMVTGIV
                     ASAFAPGRLMADEPILNPLGIEAAAGVFPVVSELVYSLTLLLLVVSVASVYLRFRRAG
                     GIERQQIKWLAYSAALLGVVVIVGTIDDLLLGVFGWWIFLVLVVTLFGIPLSIGAAVL
                     RYRLYDIDIVINRT"
     gene            complement(1326..1778)
                     /locus_tag="VHM69_16170"
     CDS             complement(1326..1778)
                     /locus_tag="VHM69_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2741981.1"
                     /translation="MAQRTVSDEELAELIRRTGEAASALIRGDIRTYITLLPHAEEYT
                     LMDPFGGEPTRGFDASSGSLQALERFFQGGEAELELVESYTSGDLVVLVVIERQHGQV
                     GGLPDQDLSLRVTWVFRREGSEWRQVHRHADPLVHGIDLEQLAALARA"
     gene            2663..2803
                     /locus_tag="VHM69_16175"
     CDS             2663..2803
                     /locus_tag="VHM69_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741982.1"
                     /translation="MSEPGVVIGYYIDWGLYRVVYMVDTGQGPRAIEEWRLSFRGKVD
                     EC"
     gene            complement(3046..3321)
                     /locus_tag="VHM69_16180"
     CDS             complement(3046..3321)
                     /locus_tag="VHM69_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2741983.1"
                     /translation="MRIAGYFVTGLGVVFIALGIVDYVLKEIAPPPEGPPQPSEPTGL
                     TAFILALARLTIALTGAPTYVSATILGVLLVVLGVTLVTLAQRRPLI"
     gene            3732..4502
                     /locus_tag="VHM69_16185"
     CDS             3732..4502
                     /locus_tag="VHM69_16185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2741984.1"
                     /translation="MSAEQPKLYTELAGWWQLVSPTEDYADEAAFFEGLFRAENAKTV
                     LELGSGGGNIAWFLKEHFTLTLTDLSDGMLAESKKQNPELEHIRGDMRTLRLGRTFDG
                     VLIHDAIMYMTTEEDLRAALATACEHCKPGGMVVIAPDWVAETFRPQTMHEGGDEGER
                     GVRYLEWVWDADPDDTEVNCEYILALKDGDELRTVVDRQIVGVFPRATWLRLLEEAGF
                     EATVLEDPSTGGERSEVLLGRRSWQPGHSAGPDPADQD"
     gene            complement(4499..5830)
                     /gene="trmFO"
                     /locus_tag="VHM69_16190"
     CDS             complement(4499..5830)
                     /gene="trmFO"
                     /locus_tag="VHM69_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX2741985.1"
                     /translation="MPDVTIIGAGLAGSEAAWQAAELGCSVELWEMRPVKETPAHRTQ
                     HFAELVCSNSLGNRSLETASGLLKAELRRLGSVILRCADANSVPAGGALGVAREDFPR
                     AVTETVYSHPNIEVIRREVTDIPEGPTVVATGPLTSDALAEKVEALSGERLYFYDAAS
                     PIIHRESLDDAMIYRASRYDKGEAAYLNCPMKEDDYYAFVDDLAAAELSPIKDFEEDL
                     YFEGCLPVETIARRGRETLRFGPMKPVGLPDPRTGEIPYAVVQLRQDDAEGHLYNIVG
                     FQTRLKWGEQRRVFRSIPGLENADFARMGVMHRNTYLPSNHMLEATMRIREDLSDKPL
                     FFAGQLTGVEGYVESTAMGHIAGSNAARLARGEETITMPRGTMMGALANYITTKEGTL
                     QPINSNWGLVPAVPKKENGRRLSKPERRQRQARTALAVLDEFMQGGFQTAV"
     gene            5952..6386
                     /locus_tag="VHM69_16195"
     CDS             5952..6386
                     /locus_tag="VHM69_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX2741986.1"
                     /translation="MGKKRVLIAATLTMAGVMFFGVPALAGTMLTATLSGAAEVPGPG
                     DPDSSGTADLNLLPKRERICYTITVQNIEPATAAHIHRGTSTVAGPIVKELKAPSDGS
                     SSGCVRSGRTLIQKIRNNPSRYYVNVHNDDFPNGALRGQLTK"
     gene            complement(6434..6571)
                     /locus_tag="VHM69_16200"
     CDS             complement(6434..6571)
                     /locus_tag="VHM69_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2741987.1"
                     /translation="MLDGIPLHRFGNPEDLKGVVIFLASPAASYITGQTVVVDGGATA
                     W"
BASE COUNT         1371 a         1993 c         2003 g         1209 t
ORIGIN      
        1 ggtgcggttt atgacgatat ctatgtcgta gagccgatag cggagtaccg ccgcgccgat
       61 ggagagcggg atgccgaaca aagtcacaac caggaccagg aagatccacc atccaaagac
      121 tccgagcagc aggtcatcaa tcgtgccgac tatgacgact accccaagca acgcggctga
      181 gtaggcgagc catttgatct gctggcgctc tatccccccc gcccggcgaa acctcaggta
      241 aacggaggca acggagacca ccagcagcag cagggtgagg gagtaaacca actcggacac
      301 gacggggaag acaccagctg ccgcctcgat gccgagcggg ttaaggatcg gttcgtccgc
      361 catcagccgg cccggagcga atgcgctagc gacgattcct gtcaccatca ggatgccgtt
      421 gatccacatg acatagcgcc agcggcgagt cagaggccgc ccgtcgggga aaagcaggaa
      481 caagaatacg aacaacgagt acacaggttc ctgaacccag gcggcgaacc aggccgccac
      541 ttctcctgcg ggaagagagc caggataggt gaccagcgcg tagatggcgt acataccgga
      601 gaagatactg aacgcgaaca agaagccgct cgcgcacagt atccacccga ttgaattatc
      661 aggccgccgg gaaacgatga gcgctccgac cgtagtggag gtaaccaccc acacagccac
      721 cggaagtacg gcgatcaagc tgccctgctc cctaaccacg agcagggaga gggcgcaaac
      781 cacagtcagc gtccacaaca accacgcaag ccatctggcg ccgctcgtcc tatctactcc
      841 tcgcccctgt tctcttgctc cttctgtcac gacacaactc tacagcacca ccgtctccaa
      901 agcgttacga aacgcgctac gaagtggagg tgatcgaaca tcgcagaacg tctcacaggc
      961 gaactccagg ctctcccagg tcgacgattc gagcgacgcc ttcagtcagg ccccatgtgg
     1021 gcgcgcctcg tcgcaagggc cgtgtgccgc ggccagagta gagcgagctg atctacctgc
     1081 cttctcgcct gacttcaacc cgatagagga agccttctcc aagctcaagc atctgctgag
     1141 gaagttcggc gcccgcggca gaaagcccct ggtcgaggcg atgggtcgag cgttggcagc
     1201 cgtgagcatc gaagacgtga gaggatcctt cgctcattgt ggctactgcg tcccggctca
     1261 gcaactataa agacgctgta tccggccttc ctgatgaggc gggtggcggt ggcacggtag
     1321 aagagtcagg cgcgcgcgag tgctgctagt tgctcgaggt ctataccatg gaccagcggg
     1381 tcggcatgtc gatgcacctg ccgccactcg gatccctcac ggcgaaagac ccaggtcacc
     1441 cggagcgaca agtcctggtc cggcagacca ccgacctggc catgctgacg ttcgatcacc
     1501 accaacacga cgagatctcc cgaagtgtat gactccacca gttccagctc cgcctcgcca
     1561 ccctgaaaga agcgctccaa ggcttgcaga ctcccgctcg atgcgtcgaa gccacgcgtc
     1621 ggttcgccgc cgaacggatc catcagcgtg tattcttccg catgcggaag aagcgtgatg
     1681 taggtgcgta tatccccgcg tataagggcc gaggcggctt ccccggtgcg ccgaatgagt
     1741 tctgcaagtt cctcgtcgct gacggttctc tgcgccatgg tcgcggcctc ctctaggggt
     1801 ataggcggtt tcgctcctgg ggacaccctt cttcaccgac tcgacgatct tctctctgag
     1861 atccatcgag tacaccctca tgcttctacc ttacccggcg catcatcgca acccgctgat
     1921 tgggcacagg tggcgagaac tcagggggta acgcccaggg cttccgcttc gaagagccag
     1981 gccaaaaccc gggtgtggcg attaccaatg tcgctaacgc agatgccggg cgaattacgg
     2041 tagaccgccc cggggataac gacggtacac tgagtggtac ttatcgaggc gatagtgcgg
     2101 gagtgggtcg tttggaggtt ggtcaggtca atgtgatgct gccaattttc cggagttagc
     2161 aacagacggc cagacgtcgg ctgtttaaaa gctgcaccag catctgaaca acaagcgcct
     2221 gggtcctacg gggctccggc cgtaatctct tgtatttccg cgtgggccag gacgcactat
     2281 tactaataga ctcggcggca aggctttcac gttaacttca ttatctggtc gccgggtgac
     2341 attccgggcc ggggtcccgt agagctccgg ccccttcgct gtatcccact cctaacgccc
     2401 gagcactccg cgcccgctct gggtttcctt cggtataaca cccatcagaa tacgaatact
     2461 tcgccggcgg tccctgtccg ccttggaacc gtggcgatca cctatttgcc cggagcagag
     2521 cgcggtctct cggaagctcc tgtagtactc tcacttcgta gccagttagg agtacagaga
     2581 gcggctaatg agcaggccat ctggggaaga tgagccccga tacgcgaagg gtgatcgagt
     2641 gtgggtccag agaggacatg gcttgagcga acccggcgtg gtgatcggct actacatcga
     2701 ctggggcttg tacagggtcg tctatatggt cgataccggg caaggaccga gggcgatcga
     2761 agagtggcgt ctgtccttcc gaggaaaagt ggatgagtgc tgacctcata tttcggcccc
     2821 tattcacaga agctcgcagg caaaaagggc gaaaaatggg ccacaatcga ggtgaggaaa
     2881 cgcagcaagc ggtagcgact tgcttccgac gaacccccgg agatttcagg aaatgttccc
     2941 aatccgccga agctagagcc tttggcgccg gtgtcaaagg tttcccgccg gcgggtccct
     3001 ctctatgaac accctctctg gtcatctgta ctcatcttga gtttgtcaga tgagcggtct
     3061 gcgctgggcc agagtgacga gggtgacgcc taagacaacc aagagtaccc caagtatagt
     3121 tgctgagacg tatgtgggcg cgccggtcaa tgctatggtg agccttgcca gggcgagtat
     3181 aaatgccgtc agcccagtcg gttccgatgg ttgtggcgga ccctccgggg gcggcgcaat
     3241 ttccttgagc acgtagtcca caatgccgag cgcgataaac accacaccga gccccgtaac
     3301 gaagtatcca gctattctca tagcaccgca tcaccctccg aataggccaa ccaccctgac
     3361 agcgtaattg ctaccgctag gatctgctcg ttcgcccgcc gaacatccta cgccaccagg
     3421 accggggacc tgcccactcc gccattctag tcccttgcgt caagcctgta atcccttgtc
     3481 aggtaaggga agatatcgaa ggtcccgctg taaaactgat tctgggtagc gcgccgttcc
     3541 ctggcccgtg tgacggctat tagagtcgcg gctccgggaa gggcttttcg tcgagtttag
     3601 cgggcaggca attcataatg tggaggaatc gctccccgcc cgggagagcg aggtcgtagg
     3661 cagcgccagc acgctattcg acctctcccg cttccaggca ggtcactacg gctgaatggt
     3721 agagttgcgg catgagtgcc gaacaaccga agctgtacac cgaactggcg ggctggtggc
     3781 aactggtgtc accgacggaa gactacgcgg acgaggcagc cttctttgag gggctcttcc
     3841 gggccgagaa cgcgaagacg gtgctcgagc ttggaagcgg tggcggcaac atcgcctggt
     3901 ttctcaaaga acactttacg ctcacgctca cagacctttc ggacgggatg ctcgcggaga
     3961 gcaagaagca gaaccccgag ctcgaacaca tcaggggcga catgcgcacg ctgcgcctcg
     4021 gcaggacgtt cgacggcgtc ctgatccacg acgccatcat gtacatgacg acggaggagg
     4081 acctccgcgc ggcccttgcc acagcctgcg aacattgcaa gccaggcggc atggtggtca
     4141 tagcacccga ttgggtcgca gagaccttcc ggcctcagac catgcacgaa ggaggcgatg
     4201 aaggcgagcg gggcgtcagg tacctggaat gggtctggga cgccgacccc gacgacacag
     4261 aagtcaactg cgaatacata ctggctctca aagatggcga cgaactccgc accgtcgtcg
     4321 accgccagat cgtcggcgtc ttcccccgtg ccacctggct gcggttgctg gaggaagccg
     4381 gcttcgaggc aacggtcctc gaagacccgt ctaccggcgg tgaacgcagc gaggtccttc
     4441 tgggccgtag atcatggcag cctggccaca gcgccggtcc tgatccagca gaccaagact
     4501 aaacggcggt ctgaaatccc ccttgcataa actcatcgag caccgcgagg gccgtcctgg
     4561 cctggcgctg acggcgctct ggcttgctca gacgcctgcc gttctccttc ttcggtacgg
     4621 cggggacgag gccccagttg gagtttatgg gctgaagcgt cccctccttg gtcgtgatgt
     4681 agttggcgag cgcgcccatc atcgtgcctc gcggcattgt gatggtctcc tcaccgcggg
     4741 caagtcgggc tgcgttggaa ccggctatgt ggcccatcgc tgtgctctcg acgtagcctt
     4801 ccacgcccgt cagctggccc gcaaagaaga gaggcttatc gctgaggtcc tcccttatcc
     4861 gcatggtggc ctccagcatg tggttcgagg gaaggtaggt attgcggtgc atgacgccca
     4921 tgcgggcgaa atcagcgttt tcgaggccag ggatgctacg aaagacacgc ctctgttcgc
     4981 cccacttcag tcgcgtctgg aagccgacga tgttgtagag gtggccttct gcgtcgtcct
     5041 ggcgcagttg cacgacggcg taggggatct cacccgttcg cgggtccggg agcccgacgg
     5101 gcttcatagg gccgaagcgt aaagtctctc tgccgcggcg ggcgatggtc tcgacgggga
     5161 ggcacccctc gaagtacaga tcctcctcga agtcctttat ggggctgagt tcggcggcgg
     5221 ccaggtcatc gacgaaggcg taataatcgt cctccttcat cgggcagttc aggtaggccg
     5281 cctctccctt gtcgtagcgg gaggcgcggt agatcattgc gtcgtccagg gactcgcggt
     5341 ggataatggg agatgccgcg tcgtagaaat agagcctctc ccctgagagg gcctcgacct
     5401 tctcggccag cgcgtccgag gtcagtggtc ctgtcgccac gaccgtaggt ccctcgggta
     5461 tgtccgtgac ctctctgcgg atcacctcga tattgggatg agagtacacc gtctccgtaa
     5521 ctgcacgcgg gaagtcttcg cgggcgaccc cgagagcgcc gcccgcgggt accgagttgg
     5581 cgtcggcgca gcgcaggatc acggacccga ggcgccgcag ttccgctttt aacaaaccgg
     5641 aagccgtctc gagagatcta ttaccgagcg agttggagca gacgagctcg gcgaagtgct
     5701 gcgtgcggtg ggccggggtc tcctttacgg ggcgcatctc ccacaactca accgagcagc
     5761 cgagctccgc ggcctgccag gccgcttcgc ttccagcgag ccctgctcct atgatggtta
     5821 cgtcaggcat ggatcttcct ccagggaaac gtcttgtgag agccgtcatt ctacgtcaag
     5881 agagggtaaa ccgtcgcccg caccgctgcg tatatccgta tgcgaagcgg tggctactca
     5941 aggggatgtt gatggggaaa aagagagttc tcatcgcggc gacgctgacg atggcaggcg
     6001 taatgttctt cggcgtcccg gcgctggccg gaacgatgct cacggccacc ttgtcagggg
     6061 cagcggaagt gccgggaccg ggcgatcccg acagcagcgg gacggccgat ctcaacctcc
     6121 tcccaaagcg ggagaggatc tgctacacga tcaccgtaca gaacatagaa ccagctacgg
     6181 cggcgcacat acacagaggt acctccaccg tggccggtcc gatagtcaag gagttgaagg
     6241 cgccgagcga cggttcctcc agcggttgcg tcaggagcgg gcgcacgctg atccagaaga
     6301 tcaggaacaa cccgtccagg tactacgtca acgtgcacaa cgacgacttc cctaatggag
     6361 ccctgagggg ccagctcacc aaatagccag tcaacatttc agcccaaggg gtcgaggtgc
     6421 tgaccggctg aaatcaccag gccgttgcgc ccccgtccac gacgacggtc tggcccgtaa
     6481 tgtaggaggc tgcgggcgag gcgaggaaga tcaccacgcc tttgaggtcc tcggggttgc
     6541 cgaaacggtg cagagggatg ccgtcgagca ttcgag
//
LOCUS       DATAIR010000687         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_255914, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000687 DATAIR010000000
VERSION     DATAIR010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255914"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VHM69_16205"
     CDS             <1..672
                     /locus_tag="VHM69_16205"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX2741988.1"
                     /translation="ADPLEVIEWAVETYGEGLALSASFGGGEGMALLDMISKVTDKVT
                     VLTIDTGFIFKETAEFREEVMRRYKLPVEVLKPALTIEEQVERFGEQMRTCTPNLCCQ
                     IRKIEPLQRALNNYDAWMTGIRREQTPQRADTKVVSWEERYGAAKIAPMPSWTEEQVW
                     DYVRAHDVPVNPLLHQGYKSIGCEPQTRPVHHDEDPRAGRWSGIDKTECGIHFVDGRI
                     SRTGA"
     gene            712..>1526
                     /locus_tag="VHM69_16210"
     CDS             712..>1526
                     /locus_tag="VHM69_16210"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX2741989.1"
                     /translation="MKFLRKDQTLNKVEKIKQERHPLEVYGAIINTYSKDLSAMDEVP
                     GEVERLKWVGIYPQKQGGDAFMMRIKVPGGVLTPEQTRVIGKLAADFAHGPIPNPHFG
                     NNFLDLTTRQDVQMHWIKMENVPEIWRRLEEVGITTVQACGDSARNVLCCPVSGLGHD
                     EVIDAYPVAQAISNYFTGNREYANLPRKFKMSVTGCLEDCAQAEINDIGMLPARLEDG
                     TLGFNVRVGGGLSDGPRMASDIDVFVRPDEAVEITRGIAQVFGELGDRENRWTA"
BASE COUNT          326 a          453 c          503 g          244 t
ORIGIN      
        1 gccgatccgc tggaggtaat agagtgggcg gtggagacct acggtgaggg cctggcgctc
       61 tcggcctcgt tcggcggggg cgaggggatg gcgcttctgg acatgatctc caaggtcacc
      121 gacaaggtca ccgtcctcac catcgacacg ggcttcatat tcaaggagac cgccgagttt
      181 cgcgaggaag tcatgcggcg ctacaagcta cccgtggagg tcttgaagcc cgcgctcacg
      241 atcgaggagc aggtcgagcg cttcggcgag cagatgcgca cctgtacacc gaacctgtgc
      301 tgccagatcc ggaagatcga gcctcttcag cgtgccctga acaattacga cgcctggatg
      361 acgggcattc gcagggagca gaccccccag agggcggaca cgaaggtcgt ctcatgggag
      421 gagcgctacg gtgccgccaa gatagcgccc atgccgtcct ggacggaaga gcaggtgtgg
      481 gactacgtga gggcacacga cgtgccggtc aacccgctgt tgcaccaggg ctacaagagc
      541 atcggctgcg agccgcagac gaggcccgtg caccacgacg aggacccgag ggcgggcagg
      601 tggtcgggta tcgacaagac ggagtgcggt atccacttcg ttgacggcag gatcagccgc
      661 acgggcgcct gaatttcaca gatcacgctt caatagagca ggttttagat aatgaagttc
      721 ttgaggaaag accagaccct caacaaggta gagaagatca agcaggagcg ccatcccctc
      781 gaggtgtatg gcgcgataat caatacatac tcgaaggacc tctcggctat ggacgaggtc
      841 ccaggcgagg tcgagcgtct gaaatgggtg ggcatctacc cgcagaagca gggcggcgac
      901 gcctttatga tgcggatcaa ggtccctggt ggcgtcctga cgccggagca gaccagggtc
      961 atcggaaagc tagcggcaga cttcgcgcac gggccgatac ccaacccgca cttcggcaac
     1021 aacttcctcg acctgacaac gcgccaggac gtgcagatgc actggatcaa gatggagaac
     1081 gtacctgaga tctggcggcg cctggaggag gtcggcatca ccaccgtgca ggcgtgcggc
     1141 gactcggccc gcaacgtcct ctgctgcccg gtctcagggc tcggccacga cgaggtcatc
     1201 gacgcctacc ccgtggcgca ggctatctcc aattacttca cgggcaaccg cgagtatgcc
     1261 aacctgcccc gcaagttcaa gatgagcgtt acgggctgtc ttgaggattg cgcgcaggct
     1321 gagatcaacg acatcgggat gctccccgcg cgcctggagg acggcacgct cggcttcaat
     1381 gtgcgcgttg gcggcggcct ctccgacggc ccgaggatgg catcggacat cgacgtattc
     1441 gtccgccccg acgaggcggt ggagatcaca cgcggcatag cccaggtctt cggagagctc
     1501 ggagacaggg agaaccgctg gaccgc
//
LOCUS       DATAIR010000688         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_255940, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000688 DATAIR010000000
VERSION     DATAIR010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255940"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VHM69_16215"
     CDS             <1..1048
                     /locus_tag="VHM69_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2741990.1"
                     /translation="VRESAHRITIADVAREAGVSRQTVSRVLNGKGEIRASTRESVMR
                     VIERLGYSPSGIARSLATNKTLTVGLVVPDITNPFFPEIARGVEDVARERGYEMFLCN
                     SGEDPEREDDVLRALQVKRVDGTIICSSRLSDERLFTRLRQQRAAVLVNRHAPPELAG
                     TVRVDDAEGTFQAVDHLLAGGRCVIGFLCGPPNSRSGIERVRGFKTALSAAGTGLDET
                     LMRPCPPNPQGGYEAALALLSERPDIDGLVCYNDLVAVGALRACAGLGSRVPDDVAIV
                     GCDDIMVAGLLSPALTTLRVPKYDIGASAARMLLDRISGRGGEDEVLLQPELVVRESA
                     PGLDPSETAEGREN"
     gene            1048..2133
                     /locus_tag="VHM69_16220"
     CDS             1048..2133
                     /locus_tag="VHM69_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2741991.1"
                     /translation="MTTGERLSFGILGCGVIGPHHARAISGLDGAEVVAVADVVPELA
                     EKLAAEYGSSWHTSLEDMLSGAEVDAVCVCTPSGMHADHAIAALESGKDVVIEKPVDV
                     TLKAADRLLDVQRATGRKVAVVSQHRFDPATLAVREAVSNGEFGRLTSGSAEVRWWRS
                     QAYYDSGGWRGTWELDGGGVLINQAIHSIDLLQWLMGPVVEVTAYTGLLAHQRIEVED
                     TAVAILEFAGGALGTIVATTAAFPGLTASIAVHGDRGSAVIDDDDLRYFHVAGGGQEG
                     DDYGGGENQAEQVMKRYGASSASSGAGADPGSLSMAHGDQIKDFIEAVGEDREPSVNI
                     EEGRKALAIITAIYESARTGEPVRIQQ"
     gene            2194..3024
                     /locus_tag="VHM69_16225"
     CDS             2194..3024
                     /locus_tag="VHM69_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2741992.1"
                     /translation="MWTLTGFADEISPELDEQLDTLAQESMKFMELRSVWNTNVLDLT
                     DVELEKVRSATTGRGVGISSIGSPIGKVSITDPFGPHLGRFRRALNAARFMNAPYVRV
                     FSFFIPEGQAPDLYRDEVLERMGAMASEAEGAGITLLHENEKEIYGDVPSRCLDILES
                     VDSSALRAAWDAANFVQCGIQHPYTDGYELLRPYVEYVHVKDALAESGTVVPAGEGDG
                     EIPETLAALRASGFDGFFSLEPHLASSGTYSGFSGPELFRKAAGAFKDLLRRQGIEWA
                     "
     gene            3148..>3780
                     /locus_tag="VHM69_16230"
     CDS             3148..>3780
                     /locus_tag="VHM69_16230"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEX2741993.1"
                     /translation="MAEETAAGILRLRDLDVLPRSVVSVAGAEFGLARPGPRVAILAP
                     SGSPVLAGFEGEGSARGDRTLVLGPTSVANLDALRALLPWLRPRPLGILASAGFGDRL
                     GLATPGHVRAMRTSGAGMAPIFAQQSIREMERTGRSPMEVLDDATWGVFAEGWREGFG
                     ADADHLKTAEDVDACVAAGYTFFTFDPGEYVEDGAEAADASALIFRLEDLP"
BASE COUNT          694 a         1109 c         1314 g          663 t
ORIGIN      
        1 agtgagggag agcgcgcaca ggatcaccat cgccgacgtg gcccgtgagg cgggggtttc
       61 aaggcagacg gtttcgaggg tgctcaacgg gaagggggaa atccgtgcct cgacccgcga
      121 gtcggttatg cgtgtgatcg aacgcctcgg gtacagcccg agcggcatcg cgcgcagtct
      181 ggccacgaac aagaccctca ccgtcgggct ggtcgtaccc gacataacca atcctttctt
      241 tccggagatc gcgcgcgggg tcgaggacgt ggcgcgggag cgcggttacg agatgttcct
      301 gtgcaacagc ggcgaggacc ccgagcgaga agacgatgtc ctgcgggccc tccaggtcaa
      361 gagggtggac gggaccatca tctgcagttc caggctgtcc gatgagcggc tcttcacccg
      421 cctgcggcag caacgggcgg cggtccttgt caacaggcat gcgccacccg agctggcagg
      481 aacggttcgg gtggatgacg ccgaaggtac gtttcaggcg gtggatcacc tgctggctgg
      541 cgggcgctgc gtcatcgggt tcctgtgcgg tccaccgaac tcgcgcagcg gcatagaacg
      601 tgtgcggggc ttcaagacgg cactttcggc ggccggtact ggtctcgacg agacactcat
      661 gcgtccgtgt cctcccaacc cgcaaggagg gtacgaggcg gcgctcgcgc tgctgtcgga
      721 gcgcccggat atcgacgggc tcgtctgtta caacgacctc gtcgccgtgg gggcattgag
      781 agcctgcgcc gggttgggat ctcgcgtgcc cgatgacgta gcgatagtgg ggtgcgacga
      841 cataatggtc gcgggcttgc tctcgcctgc gctcacaaca ttgcgcgtac ccaagtacga
      901 catcggcgcg agcgcggcca ggatgctcct cgaccgcatc agcggcaggg gtggggagga
      961 cgaagttctg ctccagccgg agctagttgt ccgtgagagt gctcctggat tggatccatc
     1021 agaaacagcc gaagggagag agaactgatg acgacgggcg agaggctgag tttcgggata
     1081 cttggctgtg gcgttatcgg accccaccac gcgcgcgcca tctcgggcct cgacggcgcg
     1141 gaggtcgtgg cggtggccga cgtagtccct gaactggccg agaagcttgc tgctgagtac
     1201 gggagctcct ggcacaccag cctggaggac atgctctcgg gagcagaggt ggacgctgtg
     1261 tgcgtgtgca ccccgagtgg catgcatgcg gaccacgcca tagcggcgct cgagtcaggc
     1321 aaggacgtgg tgatcgaaaa gcccgtcgat gtaacgttga aggcggccga ccgtctgctc
     1381 gatgtgcagc gcgccaccgg gaggaaggtc gccgtcgtca gccagcaccg cttcgacccg
     1441 gcaacgctgg cagtgcggga ggcagtgagc aacggtgagt tcggccgtct cacttcaggg
     1501 tctgcggagg ttcggtggtg gcggtcccag gcgtactacg attccggcgg ctggcgtggc
     1561 acatgggaac tggatggggg aggagtcctc atcaaccagg ccatccatag catcgacctg
     1621 ctgcagtggt tgatgggccc tgtcgtcgag gttaccgcct acaccggact tctggctcac
     1681 cagaggatcg aagtcgagga caccgcggta gctatcctgg agttcgccgg cggcgccctc
     1741 ggcaccatag tggctactac cgcggcattc ccagggctta ccgccagtat cgccgttcat
     1801 ggagatcgtg gctccgcggt aatcgatgac gacgatctca ggtacttcca cgttgccggc
     1861 gggggacagg agggggacga ctacggcggg ggtgaaaatc aggcggagca ggtgatgaaa
     1921 cgatatggcg cgtcgagtgc gtcatccggg gcgggggcgg accctggcag cctctccatg
     1981 gcgcacggag accagatcaa agacttcatc gaggcggtcg gggaagaccg cgagccgtct
     2041 gtaaacatcg aagagggtcg caaggcgctc gcgataatca cggcgatcta cgagtcagcc
     2101 cgtaccggtg aaccggtgcg catccagcaa tgaaggtcga ggcaacgccg gaagtctata
     2161 gcttgccttc cgcctagaaa ggggacatga cacatgtgga ccttgacggg tttcgccgat
     2221 gagatctctc ccgaactaga cgagcaactg gacacgctgg cgcaggagtc catgaaattc
     2281 atggagctgc gtagcgtgtg gaacaccaac gtcctcgacc tgacggacgt ggagttggag
     2341 aaggtcaggt ctgcaaccac cggacgcggc gtcgggatat cttccatagg ctcgccgata
     2401 ggcaaggtgt ccatcacgga cccgtttggg ccgcatctgg ggcgcttccg gcgcgcgcta
     2461 aacgccgcaa ggttcatgaa cgcgccatac gtgcgcgtct tctcgttctt cattccggag
     2521 gggcaggcgc cggatctcta ccgggacgag gtcctggaac ggatgggcgc gatggcgagt
     2581 gaggctgaag gggcggggat cacactcctg cacgagaacg agaaagaaat ctacggcgac
     2641 gtcccgagcc gctgcctgga catactcgaa agcgtggact cgtccgcctt gagggccgcg
     2701 tgggacgctg ccaacttcgt gcagtgtggg atccaacatc cgtataccga tggctacgaa
     2761 ttgttgcgtc cctacgtaga gtacgttcac gtcaaggatg cgctcgccga gtcaggcacg
     2821 gttgtgcctg cgggtgaggg cgacggagag atcccggaga ctctcgccgc cttgcgcgcc
     2881 tcagggttcg acggcttctt ttcgctggaa ccgcatctcg cctcctcggg gacgtactcg
     2941 ggtttcagtg gtccagagct gttccgcaag gccgccggag ccttcaaaga cctgctccgc
     3001 cggcagggga tcgaatgggc ctgacgtcgt cgaaagacgg ctacctccgg gagagccagg
     3061 atctacctgt cggaaagcga ttccgccgat ggctggtggt ttctggaaag gaggcttcgt
     3121 cagaccatga acggacgggg gagagtgatg gctgaggaga ccgccgccgg aatcttgaga
     3181 ttgagggacc tcgacgtact cccccgatcg gtcgtcagcg tcgccggcgc ggagttcgga
     3241 cttgcgcggc ccggtccgag agttgcgatc ctcgcgcctt cggggtcgcc ggtactcgcc
     3301 gggttcgaag gggagggcag tgcgcgtgga gaccgtacgt tggtgctggg tccgactagc
     3361 gtcgccaacc tggacgccct gcgcgccctc ctgccctggc tgcgccctcg gccactcggc
     3421 atactcgcct cggcgggctt cggtgacagg cttgggctcg ctactcccgg ccacgtcaga
     3481 gccatgcgta cttccggagc aggaatggca cccatcttcg cccagcagtc gatccgggag
     3541 atggaacgta cggggcgaag cccgatggaa gtcctcgacg acgcaacctg gggcgtcttc
     3601 gccgaaggct ggcgggaggg cttcggcgcg gatgccgacc acctcaagac cgctgaagac
     3661 gtggacgcgt gcgtcgctgc cggctacaca ttctttacct tcgatcccgg ggagtacgtc
     3721 gaagacgggg ctgaggccgc cgacgcttcc gccctgatct ttagactaga agatctaccg
//
LOCUS       DATAIR010000689         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_348235, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000689 DATAIR010000000
VERSION     DATAIR010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348235"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="VHM69_16235"
     CDS             complement(<1..1842)
                     /locus_tag="VHM69_16235"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2741994.1"
                     /translation="MAMTHTFGDRMPRKEDGRFIRGKGRYVDDVRLPGMLHSAILRSP
                     FAHARINSVDTSAALELPGVHAVITGNDLDAQGLAWMPTLAGDTQAVLATDKVRFQGQ
                     EVAFVVADDIYIAQDALELIDVDYEPLDPVVDSRKALDEGAPIIRDDKEGKEDNAIFF
                     WDAGDKEATEGAFQGAEVTVRQEMYYPRIHPAPMETCGAVADYDVSTGEMTVHMTSQA
                     PHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLGRPVKWME
                     TRSENLMSTSFARDYHMVGELAATREGRILGARSTILANHGAFDAHAQPGEQYPTGFF
                     PIFTSCYDVPAAYAEATGVYTNRAPGGIAYRCSFRVTEAIYLMERTMDVLADELRMDP
                     VELRRKNFVTKEQMPYVSVMGWEYDGGDYEGALQEALRIAGYEELLEEQKRAREEGRI
                     MGIGISTFTEIVGAGPGKKCHIAGIEMFDAAELRIHPTGKAILKLGVKSQGQGHETTF
                     AQIVAGELGIPAENVEVREGDTDNTPFGLGTYGSRSTPVCGAATAVVTRKVREKAKKI
                     AAHLLEAAVDDIEYEDGRFFVKGSPDQAKTMDEVAFAAYTNVPEGEEA"
     gene            complement(1842..>2272)
                     /locus_tag="VHM69_16240"
     CDS             complement(1842..>2272)
                     /locus_tag="VHM69_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2741995.1"
                     /translation="PRLLLVHFIRETLGLTGTHWGCDTSNCGACTVLLDGEPVKSCTV
                     LAVRANSHEVTTIEGLWSGTELTPVQEGFHVEHGLQCGFCTPGMIITATEFLERNPDP
                     TEAEIREAISGNLCRCTGYENIVKAVQWAARNGSTQEVGA"
BASE COUNT          327 a          753 c          737 g          455 t
ORIGIN      
        1 ggcctcctcg ccctcgggta cgttggtgta tgcggcgaag gcgacctcgt ccatggtctt
       61 cgcctgatcc ggcgacccct tgacgaagaa gcgcccgtcc tcgtactcga tgtcatccac
      121 ggcggcttcc agcagatggg ctgctatctt tttggctttc tcgcgcacct tgcgggtgac
      181 tactgcggtc gccgcgccgc agacgggcgt ggagcggctg ccgtaggtgc ctagcccgaa
      241 gggcgtgttg tccgtgtcgc cctcgcgcac ctcgacgttc tcggctggga tgccgagctc
      301 tccggccacg atctgggcga aggtcgtctc gtggccctgt ccctgcgact tgactcctag
      361 tttgaggatg gctttgcccg tcgggtggat cctgagctcc gccgcgtcga acatctcgat
      421 gcccgcgatg tggcacttct tgcccggtcc cgcgcccacg atctccgtga acgtcgagat
      481 gccgatgccc atgatgcgcc cctcttcgcg cgcgcgcttc tgctcttcga gaagctcttc
      541 gtagccagcg atccttaacg cctcctgcaa cgcgccctcg tagtcgccgc cgtcgtattc
      601 ccaacccatg acggagacgt agggcatctg ctccttggtg acgaagttct tgcgccgcag
      661 ctctacgggg tccatcctca gctcgtcggc gagcacgtcc atcgtgcgct ccatgaggta
      721 gatagcctcg gtcaccctga acgagcaccg gtaggcgatg ccgccggggg ccctgttcgt
      781 gtagacgccc gtggcctccg cgtaggcggc gggcacgtcg tagcagctcg tgaagatcgg
      841 gaagaagccg gtggggtact gctcccccgg ttgggcgtgt gcgtcgaacg ccccgtggtt
      901 cgcgaggatc gtcgatcggg caccgaggat cctgccttct ctcgtggccg cgagctcgcc
      961 gaccatgtgg tagtcgcggg cgaagctcgt gctcatcagg ttctcggagc gcgtctccat
     1021 ccacttgacg ggcctaccga gtacgaggga ggcgaccgcc gcgcacacgt agcctgggta
     1081 gatggggacc ttgttcccga agccgcctcc gatgtctggg gagattatcc ggatctggtg
     1141 ttcgggcaac cccgagacga tggcgaagag cgttctgtgc gcgtgcgggg cctgcgaggt
     1201 catgtgcacg gtcatctcgc cggtcgagac gtcgtagtcg gcgacggccc cgcaggtctc
     1261 catgggggct gggtggatgc gcgggtagta catctcctgg cgcaccgtta cctcggcccc
     1321 ctggaaagct ccctcggtcg cctccttgtc ccccgcgtcc cagaagaaga tcgcgttgtc
     1381 ttccttgccc tccttgtcgt cgcggattat gggagcaccc tcgtcgagcg ccttccggga
     1441 atccaccacc gggtcgaggg gctcgtagtc cacgtcgata agctccagcg cgtcctgcgc
     1501 gatgtagatg tcgtcggcca ccacgaaggc gacctcctgc ccctggaaac ggaccttgtc
     1561 cgtggcgagc acggcctggg tgtccccggc gagggtgggc atccaggcga ggccctgcgc
     1621 gtcgaggtca ttgccggtga tgaccgcatg gacgccggga agctccagcg cggcgctcgt
     1681 gtccacgcta tttatcctgg cgtgggcgaa ggggctgcgc aggatggccg agtgcaacat
     1741 cccaggcagc ctgacgtcgt cgacgtagcg tcccttgccc ctgatgaagc gcccgtcctc
     1801 cttgcggggc atccggtcac cgaaggtatg tgtcatggcc attacgcgcc gacctcctgt
     1861 gtactgccgt ttctcgcggc ccactggacc gccttcacga tgttctcgta gccggtgcag
     1921 cggcagaggt tgcccgagat ggcttcacgg atctccgcct ccgtcgggtc agggttgcgc
     1981 tccaggaact ccgtggcggt tatgatcata cccggcgtac agaaaccgca ttgcagcccg
     2041 tgctcgacgt gaaagccttc ctgcacgggg gtgagctctg tcccgctcca gagcccctcg
     2101 atggtagtca cctcgtggga gttggccctg acggcgagca ccgtacagct cttgaccggc
     2161 tccccgtcga ggagcaccgt gcatgcaccg cagttggacg tatcgcaccc ccagtgggta
     2221 cccgtgagcc ccagggtctc cctgatgaag tggacgagca agaggcgcgg ct
//
LOCUS       DATAIR010000690         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_69324, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000690 DATAIR010000000
VERSION     DATAIR010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69324"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VHM69_16245"
     CDS             <1..418
                     /locus_tag="VHM69_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2741996.1"
                     /translation="AHDISEELRKSRPWRNVEFEIEDGLVANGDARLLRVALWNLLDN
                     AWKFTAREPNPRIEFASTLRDGRRVYRVTDNGAGFDARYSDKLFGAFQRLHSSEEFEG
                     TGIGLATVARIVHRHGGEVWAEGKVGEGAVFYFTLR"
     gene            415..1740
                     /locus_tag="VHM69_16250"
     CDS             415..1740
                     /locus_tag="VHM69_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2741997.1"
                     /translation="MRSANDRRRDLSRLDFLRLGGLAGTALLGLSGCGGGQSRGPVEL
                     VFSHGEAPEVLSAQISRFNHKHRGEIEVKLRLAPADTGQYFEKLRTGFQAGEAGVDVI
                     SGDVIWPAQFAASGWISDLSDRFGGDLREAHLPAAINSNTHRGAVYGVPWFTDAGMLY
                     YRKDLLEKSGFSEAPETWDELAEQAEKVMRDSGTKYGFVFQGAEYEGGVVNGLEFIWN
                     SGGDVLAPGNLDKVTVDAPKAVEGLKSARGTVTGGVAPEAVPSYKEYESYSDFLDGDA
                     VFMRNWPNVYARAADPRLSKIEQHQIGVAPLPVSRAGEKAYSGLGGWNLMVNAASHEK
                     LEAAWTFIRYLSAPEQQKERAIKGGYLPTLKDCYEDHEILSKVPVITLGREAIRNVRA
                     RPATPFYQDMSLAMAERFHASLSGAVDPGRAARTLRERLEEILDNAESL"
     gene            complement(1805..2365)
                     /locus_tag="VHM69_16255"
     CDS             complement(1805..2365)
                     /locus_tag="VHM69_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2741998.1"
                     /translation="MRTMIRKKIAIMLAVAGTMLLLSSGVAFAVQVINCENGKACNGT
                     AKSDRMLGTRGSNEMNGRASRDIMGGDYGKDTMHGAQGNDTIRGGYNGDSLFGDDGSD
                     VLRGQNGKDTISGGPGNDSIGAGGGDDIVMANDGQLDSISCGLGTDVVFVDQEDLDAE
                     NTNFEDFIRLTSCETINEPEATQTPE"
     gene            complement(2463..>3868)
                     /locus_tag="VHM69_16260"
     CDS             complement(2463..>3868)
                     /locus_tag="VHM69_16260"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX2741999.1"
                     /translation="SFRCFFPDGDSTTNVEIQVRDDDGALDSDSQRVVIVNVANVAPV
                     LTPAADQNSNEGQNKPFDLGSFTDPGDDDLWTITVEWGDGSADTTFDAANPGKLAQKA
                     HTYANDGNYTVTVTVVEDGGAASDSETFNVTVANVAPTVDLTGPNASNEGDTEKYEFT
                     VTDPGDDTHTIDWDCGAGEKVQGSFEYDPTTKTGSFECRFPDGPVNTTVSVSVNDGDD
                     TGNDSIQVSVANVAPVANDDQMTTTELKEVTGNLVDNDDDPGNDIDRASLEILSINAL
                     NQTASGPLGGVVTISNLLDGNVTYLPNRGFVDIDSFRYKVCDDDGDCSSANVEVTVGP
                     VDCNAQGVILGTPGADILRGTPGNDVICSFGGNDRIYAGAGDDLLVAAEGLDRMWGEG
                     GSDVMRGGSQTDAMDAALGDDYISGDTGRDVIMARGGNDYIDLKDNSAGDAANGGAGT
                     DQCKKDTGDKTVSCELR"
BASE COUNT          635 a         1201 c         1281 g          751 t
ORIGIN      
        1 tgcccacgac atctccgagg aactcaggaa gtcccgtccc tggcgcaatg tggagttcga
       61 gatcgaagac ggcctcgtcg ccaacgggga tgcgcgtctc ctgcgggtcg cgctgtggaa
      121 cctcctggat aacgcctgga agttcaccgc tagggagccg aacccgagga tagagttcgc
      181 aagcaccttg cgcgatggac ggcgcgtcta ccgcgtcacg gacaacgggg ccggcttcga
      241 cgcgcgatat tcggacaagc tgttcggggc cttccagagg ctacattcct ctgaggagtt
      301 cgaggggacg gggatagggc ttgcgacggt cgcgcgcatc gtgcaccgcc acggcggaga
      361 ggtgtgggcc gaggggaagg tcggggaggg cgcggtcttc tacttcacgc tgagatgaga
      421 tccgcaaacg acagacgccg cgatctgagc cgtctggact tcctgaggct tggcgggctc
      481 gctgggacgg cgctcttggg gctctccggg tgcggcggcg ggcagagtag gggccctgtc
      541 gagctagtgt tctcccacgg agaagcgccc gaggtcctga gcgcccagat ctcgcgtttc
      601 aaccataaac ataggggcga gatagaggtg aagctgcgcc tggcgcccgc ggacacgggg
      661 cagtacttcg agaagctccg cacggggttt caggcaggcg aggcaggcgt ggacgtcatc
      721 agcggcgatg tgatctggcc agcccagttc gcggcgtcgg gttggatctc agacctgtca
      781 gaccgtttcg gcggggacct ccgcgaggcc cacctccccg ccgccataaa ctcgaacacc
      841 cacagaggcg ccgtttacgg tgtcccctgg ttcacggacg cggggatgct ctactaccgc
      901 aaggacctcc tggagaagtc cggcttctcg gaagcgccgg agacctggga cgaactcgcc
      961 gagcaggcgg agaaggtcat gcgggactcg ggcacgaagt acgggttcgt cttccagggg
     1021 gcggaatacg agggcggggt cgtaaacggc cttgagttca tctggaactc gggcggcgac
     1081 gtcctggctc cagggaacct ggacaaggtt acagtggatg ccccgaaggc cgtggaaggg
     1141 ctcaagtcgg cgcgcggcac cgtgaccggc ggtgtcgcgc cggaggccgt ccccagttac
     1201 aaagaatacg aatcctactc ggatttcctg gacggcgacg cggtcttcat gcgcaactgg
     1261 ccgaacgtct acgcgcgcgc ggcggacccc aggctctcga agatagagca gcaccagatc
     1321 ggggtcgcgc cgctccccgt gtcccgcgcg ggagagaagg cttacagcgg tctcggcggg
     1381 tggaacctca tggtcaacgc cgccagccat gagaaactgg aggccgcctg gacctttatc
     1441 aggtatctca gcgcgccgga acaacaaaag gaaagggcga tcaaaggcgg ctacctgccc
     1501 acgttgaagg attgctacga ggaccacgaa atcctgtcga aggtgcccgt catcaccctt
     1561 ggcagggagg cgataagaaa cgtccgcgcg cggccggcca cgcctttcta ccaggacatg
     1621 tcccttgcga tggccgagag gttccacgcc tccctttccg gcgctgtcga cccagggaga
     1681 gcagcgcgga cgctgcgcga gaggctagaa gagatactgg acaacgccga atcgctctga
     1741 cagacgaagg ccggacccct gaggagtccg gccttctcgt agtcctgtcg ccgggcgacc
     1801 tcccttactc cggagtctga gtggcctccg gctcgttgat ggtctcacag ctggtaagcc
     1861 gtatgaagtc ctcgaaattc gtattctcgg cgtccaggtc ctcctggtcg acgaagacga
     1921 cgtcggtacc gagcccgcag ctgatcgagt cgagctgccc gtcgtttgcc ataacgatgt
     1981 catcgccgcc tccggcgcct atggagtcgt taccaggccc accgcttatg gtgtccttac
     2041 cgttctgacc cctaagaacg tcacttccgt catcgccgaa aaggctgtcg ccgttgtatc
     2101 cgccgcgtat cgtatcgttg ccctgggcac cgtgcatggt gtccttacca taatcaccgc
     2161 ccatgatgtc cctgcttgcc cgaccgttca tctcgtttga gcctctcgtt cccagcatcc
     2221 tgtcggactt cgcggtaccg ttgcaggcct ttccgttctc gcagttgatg acctgcaccg
     2281 cgaaggcgac cccgctgctc aagaggagca tcgtacccgc taccgccagc atgatcgcta
     2341 tctttttcct tatcatcgtc ctcatcttgc tctcctttct tgctttctct ttcccgcccg
     2401 ccggggctgg tccccggcgg gctcccgcgt tcctcagggg atttccgctc ccctcgggct
     2461 ctctaccgca gctcgcaact cacggtcttg tcccctgtgt ctttcttgca ctgatccgta
     2521 ccggctccgc cgttggcggc gtctcccgcg gagttgtcct tgagatcgat gtagtcattg
     2581 ccgccgcggg ccatgatgac gtcgcggcca gtgtcgcccg agatgtagtc atcgccgaga
     2641 gcagcgtcca tcgcgtcggt ctggcttccg ccgcgcatta cgtccgagcc gccctcgccc
     2701 cacatcctgt cgaggccctc agcggcgacc aggagatcgt cgccggctcc ggcgtagatg
     2761 cggtcgttgc cgccgaagga gcagatcacg tcgttgccag gggtgccgcg caggatgtct
     2821 gcgcccgggg tgccgaggat gacgccctgt gcgttacagt cgacggggcc gaccgtgacc
     2881 tcgacgttgg ccgaggagca gtccccgtcg tcgtcgcaga ccttgtagcg gaaggagtcg
     2941 atgtccacga agccgcggtt gggcaagtag gtcacgttgc catcgagaag gttgctgatc
     3001 gtgaccacgc cgccgagcgg tcctgaggcc gtctggttga gggcgttgat gctgaggatc
     3061 tcgagcgacg cgcggtcgat gtcgttgcca ggatcgtcgt cgttgtcgac gaggttgccc
     3121 gtcacctcct tgagctcggt cgtcgtcatc tggtcgtcgt tggccaccgg cgcgacgttg
     3181 gcgacgctca cctggatcga gtcgttgccc gtgtcgtcgc cgtcgttgac ggagacggag
     3241 accgtggtgt tgacaggacc atcggggaag cggcactcga aggagcccgt cttcgtggtc
     3301 gggtcgtact cgaaggagcc ttggaccttc tcaccggcgc cgcagtccca atcgatggta
     3361 tgcgtgtcgt cgccggggtc ggtcaccgtg aactcgtact tctcggtgtc gccctcattg
     3421 gaggcgttgg gcccggtcag atcgaccgtt ggcgcgacgt tggcgaccgt gacgttgaac
     3481 gtctctgagt cggaggccgc gccgccatcc tcgacgaccg tgacggtgac ggtgtagttg
     3541 ccgtcgttgg cgtaggtgtg agccttctgc gcgagtttgc cagggttggc cgcatcgaag
     3601 gtcgtgtcgg cggagccgtc gccccattcg acggtgatgg tccagaggtc gtcatcgccc
     3661 gggtccgtga aggagccgag gtcgaagggc ttgttctggc cctcgttgga gttctggtca
     3721 gcagcggggg taaggaccgg cgcgacgttg gcgacgttga cgatcacgac ccgctggctg
     3781 tctgagtcga gcgcgccgtc gtcgtcgcgg acctggatct cgacgttggt ggtcgagtca
     3841 ccgtcgggga agaagcacct gaagctgc
//
LOCUS       DATAIR010000691         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_256119, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000691 DATAIR010000000
VERSION     DATAIR010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256119"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VHM69_16265"
     CDS             <1..720
                     /locus_tag="VHM69_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742000.1"
                     /translation="ADGSNKRKIPNTTHSNFEIGRYGSYGGNPAFSPGSRKIIFDKGK
                     NIYTINVDGTRLTRITNALAGQGPNEDKPTFVHPVWSPVSNKIAFSWLWGGAREIHVM
                     ALSDLSNKQFLTPESRVTPAGIGVYRPDWSPDGTRLTYECSDDPCDESGGTHTYNPEI
                     FKVNADGSFNTRLTTNPADDSYPAFSPTGGKIVFSSNRPAPKDGSTDYELYIMDADGH
                     NVRQLTNNSALDILPDWQPVQ"
     gene            885..2825
                     /locus_tag="VHM69_16270"
     CDS             885..2825
                     /locus_tag="VHM69_16270"
                     /inference="COORDINATES: protein motif:HMM:NF023375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX2742001.1"
                     /translation="MLIVGIGTRPADAAATTYTLTDLGTLGGASIARDINDSGQVAGQ
                     SQNTSAQNRAFLWENGQMTNLGTLGGPTSAARGIDGSGRVVGFSRISSANNQMRAFLW
                     ENGQMTNLGTLTGFSSSEAWHINDTGVAVGRSFNSSSQGRAVLWENGQIKDLGASLNT
                     PYSEAWSINNLGQVVGESGNVDQQAKAFLYDKDTGEVTDLDALLDRRVFPHSYSEAMG
                     INNHGQVVGWSYRIPINDPPSTPSGPEGKAFLYEKGADGTETMLPLNPLDGDLYSRAR
                     DIDEYGRVVGWSRGTNGNEGEQFSAVLWEDEKVTDLNSLIPTDSPLKLLDAYAISGSG
                     KIVGSAFTKENQLRGFLLTPNDSTAPNTSATHSPEPNAAGWNNEDVTITLDATDEGGS
                     NVEKITYSASGAQPIEDRTVHKDSTQISITAEGETTITYYATDHAGNAEEPKTLTVML
                     DKTAPEVNIATPAEGAEYRLGEVVTADYTCSDDGSGVDSCAGPVPVGGNIDTAMIGQK
                     TFAVEAVDRAGNVASESQNYSVVYDFGGFFRPVDNPDVLNRVQAGSAIPVKFSLSGDQ
                     SLDVFAEGYPKSQQIECDSTAPVDNIEQTVTAGDSGLYYDASLDQYTYVWKSARAWSG
                     TCRQLVMKLDDGTVHRANFKFR"
     gene            3065..3817
                     /locus_tag="VHM69_16275"
     CDS             3065..3817
                     /locus_tag="VHM69_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2742002.1"
                     /translation="MYGENAPPSWARLPGARCRAVEHLTKLDGFKEFELLEGLDEGFF
                     QDLAIAAEVLDLGAGTSLYREGEPSGAVYFMREGRVKLYRSSGGPKARDQILGVVGDG
                     SVLGLGAALEGRPQSQGATALTECVLYAVFRDDLFEIMGRFPGGAVRLARALATRSRE
                     LEDLVGDLVFRSAPQRVARMLLNLATEEGRVTKRGVVFAPSLSRQEMAEATGISREAL
                     SRALSRLAREGILDLGGKTITILNPTELRSRT"
     gene            3969..4202
                     /locus_tag="VHM69_16280"
     CDS             3969..4202
                     /locus_tag="VHM69_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2742003.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIYPEDEVPDDQESYITKAADYFE"
     gene            4260..>4667
                     /locus_tag="VHM69_16285"
     CDS             4260..>4667
                     /locus_tag="VHM69_16285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2742004.1"
                     /translation="MTFEKIGLRASIITRMDDGSWRTLGREYVYRNPWCAFRVDDVEV
                     PGGMRIEYGVLESGGFAAVVPVTEGGDVVLVRQWRQPLGAFTLELPSGGVDADEEPEQ
                     AARRELFEETGYRAEGLEHLTSVHTSTGRSTEVC"
BASE COUNT         1007 a         1405 c         1428 g          827 t
ORIGIN      
        1 gccgacggct ctaataagag gaagattccc aacaccaccc attccaactt tgagataggt
       61 cgctatggtt cttatggtgg caaccctgcc ttctcgcccg gtagtcgcaa gatcatcttc
      121 gacaagggta aaaacatcta caccatcaac gtggacggta ccaggttgac ccggattacc
      181 aatgccttgg caggccaagg ccctaatgaa gacaaaccaa cgttcgtgca cccggtatgg
      241 tctccggtta gcaacaagat agccttttcg tggttgtggg gtggggccag ggagatacac
      301 gtgatggctc tgtccgacct gtcaaacaag caattcctga cacctgaaag cagggttact
      361 cccgcgggga tcggtgtcta caggcccgac tggtcgccgg acggtacccg gctcacctac
      421 gaatgctctg atgatccttg tgacgagagc ggaggcaccc acacttacaa ccccgagatc
      481 ttcaaggtca acgccgatgg ttctttcaat actcgcctta ccaccaatcc cgccgacgac
      541 agctatcctg ccttctcgcc caccgggggt aagatcgtct tctctagcaa ccggcccgca
      601 cctaaggatg ggagcacgga ctacgaactc tacattatgg atgcagacgg tcacaacgtg
      661 cggcagctaa ccaacaactc ggccctggat attctccccg actggcaacc ggttcagtag
      721 gggagtgaat acagaggatg gttaggagaa tcgaatatgg ggaggttcgt aattgtcact
      781 gtgagccgga gaccactttc cccactaaag aaaggaaaat tagagtgaga aagacagtcc
      841 taatgtttgc agcgacgtga tgcgctgcgt tcctggcctt cgctttgctt atagtaggta
      901 tcggcacgcg accagccgac gccgcggcaa caacctacac ccttaccgac ctcggcacgc
      961 tcggaggcgc aagcatcgct cgcgacatca atgactccgg gcaggtagcg gggcagtcgc
     1021 agaatacttc ggcgcaaaat cgggccttcc tctgggagaa cggccagatg acgaacctcg
     1081 gtaccctcgg agggccgacc agcgctgccc gcggcatcga cggctccggt cgggtggtcg
     1141 gtttctcgcg catctccagc gcaaacaatc agatgcgggc cttcctgtgg gagaacggcc
     1201 agatgacgaa cctcggtacc ctcaccggct tctcctccag cgaagcctgg cacatcaacg
     1261 acaccggagt ggcggtcggc aggtcgttca attcctctag ccagggccga gcggttttgt
     1321 gggagaacgg ccagatcaaa gacctcggcg cgtctctcaa tactccttac agcgaggcct
     1381 ggagcatcaa caacctcggt caggtggtcg gggagtcggg taacgtcgac caacaggcta
     1441 aagccttcct ctatgacaag gacacaggtg aggtgactga cctcgacgcc ttgcttgacc
     1501 gccgcgtttt cccgcattcg tacagcgagg ccatgggtat caacaaccac ggccaggtgg
     1561 tcgggtggtc ttacagaatc cctatcaacg atccacctag caccccttcc ggtcccgagg
     1621 gcaaggcttt tctctacgag aagggtgcgg atggcacaga gacgatgcta cctctcaacc
     1681 ccctggacgg agatctctac agtagagctc gtgacatcga cgagtacggc cgggtcgtcg
     1741 gatggtcgcg aggcaccaac ggaaacgaag gcgagcagtt ctcggccgtc ctctgggagg
     1801 acgaaaaagt aacggatctc aattccctga tacctaccga ttcgccgcta aaactgctcg
     1861 atgcctacgc gatcagtgga tcagggaaga tcgtcggttc ggcctttaca aaggaaaatc
     1921 agctcagagg cttcctcctg acgccgaacg actccaccgc acctaacacc agcgccaccc
     1981 actcgcctga gcccaacgcc gccggctgga acaatgaaga cgtcaccatc acgctcgacg
     2041 ccacggacga aggcggctcg aacgtcgaga agatcaccta ctccgcaagc ggcgcccaac
     2101 ccatcgagga ccggaccgta cataaggact cgacccagat cagcatcacg gccgagggcg
     2161 agacgaccat tacctactac gccacggacc acgcgggcaa cgccgaagaa ccgaagactc
     2221 tcaccgtcat gctcgacaag accgcgcccg aggtcaacat cgccacaccg gctgaaggcg
     2281 ccgagtatag actcggagaa gtagtcacgg ccgattacac ctgctccgac gacggctccg
     2341 gagttgactc gtgcgccggc ccggtacccg tcggcggcaa tatcgacacc gcgatgatag
     2401 ggcagaagac tttcgctgta gaggctgtcg acagggcggg caacgtcgcc tcagagagcc
     2461 aaaactacag cgtcgtctac gacttcgggg gcttctttag gcctgtcgac aaccccgacg
     2521 tgctaaatcg tgtacaggcg ggtagcgcca tccccgtgaa gttcagcctg agcggggacc
     2581 agagcctcga cgtcttcgct gaaggctacc ccaagtccca acagatagag tgcgactcga
     2641 cagcccctgt agacaacata gagcagaccg taaccgccgg agacagcggt ctttactatg
     2701 atgctagcct cgatcagtac acgtatgtgt ggaagagcgc cagggcgtgg agcggtacgt
     2761 gcaggcagct ggtcatgaag ctcgacgacg gcaccgtcca cagagccaac ttcaagttca
     2821 ggtaggggcg aatcctgacc aggagccgga gtgaataccg ctccggctcc ctcctgcatt
     2881 gctgaaaacg tagagcttgt ctgaaggacg aagccaagga ccttgcgacg gtaccctgtc
     2941 aagtctcgaa tacgtttgca gaggtggtgg aggaagcaga gaccgggctg gcgtactgca
     3001 attgttcttc acttggcgta taaagggtct gtgaacgcac gtgagctgga aggaacacga
     3061 aacgatgtac ggtgagaacg ctcctccttc ctgggcgcgg ctgcctggcg cgcgctgccg
     3121 ggccgtcgag cacctgacga agctcgatgg gttcaaggag ttcgagcttc tcgaggggct
     3181 cgacgaaggg ttcttccagg acctcgcgat agcggctgag gtgctggatc tcggggctgg
     3241 cacctccctg tacagggagg gtgagccctc gggggccgtg tacttcatgc gcgagggacg
     3301 cgtcaagctc taccgttcct ccggcggccc gaaggctagg gaccagatcc ttggcgtggt
     3361 cggcgacggc tccgttctcg gcctcggggc tgccctcgaa ggccgcccac agagtcaggg
     3421 cgccacggcc ctgacagaat gtgtgctcta cgccgtcttc cgcgacgacc ttttcgagat
     3481 catgggccgc ttcccaggcg gcgccgtcag gctcgcaagg gccctggcca cgaggtcccg
     3541 cgagcttgag gacctcgtcg gcgacctcgt cttccgcagt gccccgcaga gggtggcgcg
     3601 gatgttactg aacctcgcca cggaggaggg ccgcgtcacc aagaggggcg tcgtcttcgc
     3661 gccctctttg agccgccagg agatggcgga ggcgaccggc atctcgcgcg aggcgctctc
     3721 gcgcgccctc tcgcggctcg ccagagaggg aatcctcgac ctcggtggca agaccattac
     3781 aatcctcaat ccaaccgagc tccgctcccg tacctgagaa cggctcgcag caaggatggt
     3841 ttttgatcca gatcacaccg ccgtatgacc ctggtcatgg gctgccatga tactggtcaa
     3901 cggcgggtgt tgagggtggt ggcgggctgg tataaagcgg ctacgagaga gcccgagagg
     3961 aggaccctgt gacctacgtg atcactgagc cgtgtatcgg cacgaaggac cagagctgcg
     4021 tcgaggtctg cccggtagac tgtatctacg acgccggcga ccacttcatg atcaaccccg
     4081 aggaatgcat cgactgcggg gcttgcgagc cggagtgtcc ggtcgaggcc atctaccccg
     4141 aggatgaagt ccccgacgat caggagagct acatcaccaa ggccgccgac tacttcgagt
     4201 agaggagagc ccggcgatat gcggaggagg ggcccacggg tccctcctct tgcttttgcg
     4261 tgacgttcga gaaaatcggc ctacgagcct ccataatcac ccgcatggat gacggaagct
     4321 ggcggacact ggggcgggag tacgtgtacc ggaacccgtg gtgcgccttc cgcgtggacg
     4381 atgtggaggt gcccggcggt atgcggatcg aatacggcgt cctggagagc ggtggtttcg
     4441 ccgccgtggt gcccgtcacg gaggggggag acgtggttct ggtgcgccag tggagacaac
     4501 ccctcggcgc attcacgctg gagttgccga gcggcggagt cgatgctgac gaggagcccg
     4561 agcaggcggc ccgtagggag ctcttcgagg agacgggtta ccgggcggag ggactggagc
     4621 atctcacgtc ggtacacacg agcacgggac ggagcacgga ggtctgc
//
LOCUS       DATAIR010000692         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_210674, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000692 DATAIR010000000
VERSION     DATAIR010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210674"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VHM69_16290"
     CDS             <1..1006
                     /locus_tag="VHM69_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2742005.1"
                     /translation="VARLATADSSGKPHAVPVCFAIAQPSIYIALDEKPKDVPVSRLK
                     RVRNIIENPDVALVADRYTEDWSRLAFVMVRGRAELLDSDTMEHSAAVRLLRGKYHQY
                     EAMRIEENPVIAIRLEKAASWGALDEAESSEPPILDTIHGRRSIRRYLPKMVPDETIE
                     QVLEAARWAPSPHGRQPWRFAVIRRVETKERLADAMGEEWRSNLEMDGQDAAVVEKRL
                     EGSRRRLLDAPVLVLLCLYLEDLDVYPDAERQRGETTMAVQSLGAAAQNALLAAYDLG
                     LDAGWMCAPLFCPDKVVEALGLDPKLVPHALLTLGYADGDPPKRRGRKTLDQLVVYRD
                     "
     gene            complement(1143..>1551)
                     /gene="fbiC"
                     /locus_tag="VHM69_16295"
     CDS             complement(1143..>1551)
                     /gene="fbiC"
                     /locus_tag="VHM69_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase"
                     /protein_id="HEX2742006.1"
                     /translation="REIQAETGGFTEFVPLPFVHMGAPLFLQGRSRKGPTFAEALKMH
                     AVGRIALHGYIDNVQVSWVKMGTEGAKACLNAGCNDLGGTLMNENISRAAGASHGQEM
                     LPSELEAMIQEIGRIPRRRTTLYGVPEREYSVG"
BASE COUNT          283 a          500 c          494 g          274 t
ORIGIN      
        1 ggtggcccga cttgcgaccg cggacagttc gggaaagccg cacgccgtcc ccgtctgctt
       61 cgcaattgcg cagccttcga tctacatagc cctcgacgag aaaccgaagg acgtgcccgt
      121 atcccgcctg aaacgcgtca ggaacatcat cgagaacccc gacgtggccc tcgtcgccga
      181 ccgatacacc gaggactgga gccggctcgc tttcgtgatg gtgcgtggcc gcgcggaact
      241 tctggattct gacactatgg agcactcggc ggccgtgagg ctgctgcggg gcaagtacca
      301 ccagtacgag gcgatgagga tagaggagaa ccccgtcatc gctattcgtc ttgagaaggc
      361 agcctcgtgg ggcgcgctgg acgaggcgga gtcctccgag ccgccgatcc tcgacaccat
      421 acacgggcgc cgttcgatcc ggcgatatct tcccaagatg gtgcctgacg agacaatcga
      481 gcaggtactc gaagccgccc gctgggcccc ttctccacac gggcggcagc cctggcgctt
      541 cgccgtgatc agaagggtgg agacgaagga gcgtctggcc gacgcgatgg gcgaggagtg
      601 gcgctccaac ctggagatgg acgggcagga tgccgccgtc gtcgagaaac gcctcgaagg
      661 ttccaggaga cgcctcctcg atgcgcctgt actggttttg ctctgcctgt acctggagga
      721 cctcgatgtc tacccggacg cggaacggca acgcggcgag accacgatgg ccgtccagtc
      781 tctaggggct gctgcccaga atgcgttgct cgcggcatac gacctcggac tcgacgcggg
      841 ctggatgtgc gccccgctct tctgtcccga caaagtcgtc gaggcgctcg ggctggaccc
      901 caagcttgta ccccatgcgt tgctcacgct cggatacgcg gacggggacc cgccgaagcg
      961 ccggggccgg aagaccctcg accaactcgt cgtctacaga gactgagtca accgcagggt
     1021 catgaacgac gtgagtgcaa cgtccacgcc gacacgggcg ttgcagaacc cggattccga
     1081 cggatcatac ttgccgcgac gctcatcgac cgaaggtgcc ttttcgacga gccgatagcc
     1141 tctcacccca cggagtattc tcgctcgggt acgccgtaga gcgtggtgcg gcgacgtgga
     1201 atgcgtccga tctcctggat catggcttcc aattctgatg ggagcatctc ctggccgtgg
     1261 ctggctccgg cggcacgcga gatgttctcg ttcatgagcg tgcccccgag gtcgttgcag
     1321 cctgcgttca ggcaggcctt ggccccttcg gtacccatct tgacccacga gacctggacg
     1381 ttgtctatgt agccgtgcag cgctattcga cccacagcgt gcatcttgag cgcctcggcg
     1441 aaggtcgggc ccttgcgaga gcggccctgc aaaaatagcg gcgctcccat atggacgaaa
     1501 ggcagcggca cgaactcggt gaagccgcct gtctcggcct gtatctcgcg c
//
LOCUS       DATAIR010000693         9107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_256287, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000693 DATAIR010000000
VERSION     DATAIR010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256287"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHM69_16300"
     CDS             <1..489
                     /locus_tag="VHM69_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742007.1"
                     /translation="VPRRREEERLDPHVYKRGINGLTVEEALRRAVPRVMDILRRSGE
                     EYSEFEEQLVRQTVRACLISQVSRANLYRIAGILVADRRDEGAYIDTSLALAYVAALR
                     EAFITEISVGERVRTPLGFGEVRYVSEDGANAAIRLDSAHEVWLPVQRVSRPDVFARR
                     AS"
     gene            555..1475
                     /locus_tag="VHM69_16305"
     CDS             555..1475
                     /locus_tag="VHM69_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2742008.1"
                     /translation="MEKSKARVICNPQSRGGAYDPDEIRDHLDGLAVEWVQTEGPDEV
                     IAAAKEWREGLLIVAGGDGTINDVVNGLGRAGFPDGVTLGILPSGTGNDLAATLCIPE
                     DHDLAKGVILQNRVQKLDVARVRSDGVGERFFLNVATGGLGAEISNANEGEFKKRWGK
                     LSYLRASLEVARDFDVRELTLYLDGEPQDLRAVNVVVANCRYAGGGWLAAPKANPEDG
                     LLDVVVIETLGMAELLKLAPASLAKFSYLDNEGVYFARAKEIRVETQPPGLEFTTDGE
                     VIGDEPAEFSVVPRALKVLVGPEYVPEPAG"
     gene            1485..1727
                     /gene="moaD"
                     /locus_tag="VHM69_16310"
     CDS             1485..1727
                     /gene="moaD"
                     /locus_tag="VHM69_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX2742009.1"
                     /translation="MIKVLLFGAAADRAGTRETELPVEDGVTLEQLWPLLAERHPGLA
                     PMRDTLAFAVNGEYARMDAVVTSGDEVAILPPVSGG"
     gene            1728..2162
                     /locus_tag="VHM69_16315"
     CDS             1728..2162
                     /locus_tag="VHM69_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX2742010.1"
                     /translation="MFAIVEEPLDIGPLIGNALRPECGAIATFVGTTRIDESEGASVE
                     YLEYEAYRPMADRKLEEIGAEIMDRWHVGHVAIVHRVGRVDPGEASVAIVVAAPRRGP
                     AFEASRYAIERIKEIVPIWKREVWSDGYVWVGSQVGTRSGAM"
     gene            complement(2155..2526)
                     /locus_tag="VHM69_16320"
     CDS             complement(2155..2526)
                     /locus_tag="VHM69_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX2742011.1"
                     /translation="MEADNEPREQGGTEIREHLANERTLLSWVRTGVSLISIGLVVER
                     AGALTDARMKVGSTSASELFGLALALLGALTLIIGTVQFLSNRRRISAGDFEPSVAAY
                     LIIVAGSLAFAGAFVVYVLFT"
     gene            complement(2680..3495)
                     /locus_tag="VHM69_16325"
     CDS             complement(2680..3495)
                     /locus_tag="VHM69_16325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2742012.1"
                     /translation="MTSDPALARQVHEAWEKKAAFWDEKMGEGNAFQRVLIGPATERL
                     LRLRTGETVLDVACGNGVFSRRLAELGARVVAIDFSEKFVELARTRTEKAGYGEAVEY
                     LVTDATDEEQMLALGEGRFDAAVCNMALMDMPVIDPLMLGLRRLLKPKGRFVFSVQHP
                     AFNSNAVTLVLEEEDRDGALVETYSVKVSDYLHVPPGMGGGMPGEPVPHPYFHRPLRE
                     LFGACFRAGFAVDGMEEPSFAEPEGGPRPATRALDWENLVGIPPVLVARARPA"
     gene            3837..5129
                     /locus_tag="VHM69_16330"
     CDS             3837..5129
                     /locus_tag="VHM69_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX2742013.1"
                     /translation="MDRRIGLEQEFFLLEDSGLPSRRADEFLERCRVLTEEESGGAAC
                     FAPEFVKALVEINTPPVHTLSDLEREYARNLRLALRTARSLGLRLYPLGTYPLPMVPV
                     IRDGLDYSVQANTVGPERFADAGRCAGTHLHLELQTGTVDAGAGISPASSVAARDELL
                     NLYNLATALDPALVALTRSCPYFEGRRSGLANRIVHYRGSAIFGWKGVYTDLPQVGAL
                     LPYADDAEHLIRQQFTRYKAWLTAMDKAGVKRHLFAEAGGDLLRPAWNPVRLNRHGTL
                     ELRGMDSNYPEVTLTAAAVILGAADRVRRNGLEVVPDDDVLTFESTEDALRVPGFGRL
                     GGELFYAAVTRGVNDETVATYLDSVLDFAGVEEERLARLSRQRRTTGVYPTTEADILE
                     EYASDVENLSEDSGLRIVLKACDALEAEVSGLAGSPAP"
     gene            5242..6000
                     /locus_tag="VHM69_16335"
     CDS             5242..6000
                     /locus_tag="VHM69_16335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2742014.1"
                     /translation="MSEPKRELQGKAALVTGASSGLGRATAISLARAGADVALVARSP
                     EELESAREEASKTGGRTLALPVDLARVDEAKRAIGETVEAFGRLDILINAAGTDAPGT
                     VEELDVEDWDRTLSVNLRAPFVLSRAAFPRMREAGGGTIVNISSVAGKKGWANASAYC
                     ASKFGLTGFTEALADEGREHGIRAIVLYPGAMATNWGAFSPEERRRSEPDEAPPSRVL
                     QPERVADFIAWFVASPPEFVLTEGIIVPIEEGLP"
     gene            6076..6567
                     /locus_tag="VHM69_16340"
     CDS             6076..6567
                     /locus_tag="VHM69_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2742015.1"
                     /translation="MGKDRAASDMDFGVRVAAVVEREGDLLLVRHQKPDRDPYWVLPG
                     GRLEPGETIPECASREVAEETGLTASFSGVLYLGEFLREGRHTIDVVARVELEGNEVA
                     SLGSDPEVAPGTEPTLTDLRWVSVDELREIQLLPASIKKRLLRDADSAWTPDDTYLGN
                     VDD"
     gene            6536..7282
                     /locus_tag="VHM69_16345"
     CDS             6536..7282
                     /locus_tag="VHM69_16345"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2742016.1"
                     /translation="MTPISVTSMTDPQTALLSVVLLDAAVAGFVIGGFWIAVSLMRSF
                     GKKIGYSLAPLGFSKPRGGIPSGIRVGIAVGVGAVLLSMIVNPLSVLVLERLGYPTKS
                     TVQQPLMEGLVGWVRESPGVAVPTILLVVVIFGPAVEELVFRGIVFNGLYRLGAMIST
                     KSVRKESSRVPSGKALFAFSALASSVLFAMLHLEPVLLPVLLILGMVLCELFRRTVSL
                     LPSFVAHATFNSFATSLIILNGLNVLNIPI"
     gene            complement(7470..8420)
                     /locus_tag="VHM69_16350"
     CDS             complement(7470..8420)
                     /locus_tag="VHM69_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742017.1"
                     /translation="MRIRPIHADEIDLFVEAGSTGHNEEVKQYVEGMFAAGAMRPGWC
                     FVIEEEGRPLGRVALWALPGADRPLALVLLDVPWEEEDLTVSVPLLRDVLDRARALGT
                     EEIEHVLDSPPMYPQFQSHQERRSELLDVIGFALKRETIRFEWRGKKTPAVSGRLDFR
                     ALDEVGDEAFADAMARVSEGTLDRQIQDERERLGSGQAARVFFEDARRVKHEPSWWQL
                     AYTPDGDLVGLVMPVEPPAFLTIFYVGVVPAMRGRGYVDDLLAAGTATLLAAGSGAGG
                     RPLLADTDIANAPMAAAFRRAGWEEFARRREYGLDLAGDS"
     gene            complement(8564..>9107)
                     /locus_tag="VHM69_16355"
     CDS             complement(8564..>9107)
                     /locus_tag="VHM69_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2742018.1"
                     /translation="VDHELPVSTFAARCVASSEATPYAVVLAGLAALGGVKHGGEIEL
                     VEAFLREVEDTGDARAVISGRLRRGERIPGFGHSLYPEGDPRGAALLRLTAEAYPEGP
                     AVALHEAVAGEVLQLMGELPTVDFALATVSRVLGLPAGGALALFALGRTVGWIGHAIE
                     QYQSDSLIRPRARYVGEQPR"
BASE COUNT         1647 a         2896 c         2925 g         1639 t
ORIGIN      
        1 gttccgcggc ggcgggagga ggagcgttta gacccgcacg tttacaagcg tgggatcaac
       61 gggttgacgg tcgaggaggc attgcgtcgg gcggttccca gggtgatgga cattttgagg
      121 aggagtggcg aggagtactc cgagttcgag gagcagttgg tacggcagac tgtacgggcg
      181 tgtttgatct cgcaggtttc gcgggcgaac ctctaccgca tcgcgggcat cctggtcgcc
      241 gacaggcgtg acgagggagc gtacattgat acttcgctcg cgctcgccta cgtcgcggcg
      301 ctcagggagg cgttcatcac ggagatctcg gtgggtgagc gggtacggac gccgctcggc
      361 ttcggtgagg tccgctacgt ctccgaagac ggcgccaacg ccgccatcag gctcgacagc
      421 gcgcacgagg tttggctccc cgttcagagg gtttcgcgcc ccgacgtctt cgcgcgccgc
      481 gcttcctagg gcgatcacag accggaggca ttccggtaga ctaggtgcgt cgtagaggcc
      541 acaaagggcg cgccatggag aagtccaaag cacgggtcat ctgcaatcct caatcccgtg
      601 gcggagctta tgatccagat gagatccgcg accatctcga cggccttgcg gtcgagtggg
      661 tccagactga aggacctgac gaagtcatag cggcagcgaa ggagtggcgt gagggcctgc
      721 tcatcgtcgc cggcggagac ggcacgatca acgacgtggt gaacgggctc ggcagggccg
      781 gtttccctga tggcgtgact ctcggcatcc tcccgtcggg gacaggcaac gatcttgccg
      841 ccacgctgtg catcccggag gaccacgacc tcgcaaaagg cgtcatcctc cagaacagag
      901 ttcagaagct cgacgtggcg cgggtgaggt cggatggggt cggggagcgg ttctttctca
      961 acgtggcgac aggggggctc ggggcggaga tctccaacgc caacgaaggg gaattcaaga
     1021 agcggtgggg caagctctcc tacctgcggg cctcgctgga ggtggcgagg gatttcgatg
     1081 tgcgcgaact cacgctctac ctcgacggcg agccgcaaga cttgcgggcg gtgaacgtgg
     1141 tcgtcgccaa ctgccgctac gcgggcgggg gctggctcgc cgcgccgaaa gccaacccgg
     1201 aggacggact gctcgacgtc gtcgtcatcg agactctggg gatggctgag ctgctcaagc
     1261 tcgcgcccgc gtctctggcg aagttcagct atctggacaa tgagggcgtg tacttcgcgc
     1321 gggcgaagga gatccgggtc gagacacagc cgcccggcct ggagttcacg accgatggcg
     1381 aggtcatagg cgacgagccc gccgagttct ccgttgtccc ccgcgcactg aaagtcctgg
     1441 tcggaccgga gtacgtcccc gagccggcgg gatagaatta ggccatgatc aaagttctcc
     1501 tcttcggtgc cgccgcggac agggccggga cccgcgagac cgagttaccc gtggaggatg
     1561 gcgtaaccct cgagcaactc tggcctctgc tcgccgagag gcatccgggt ctcgccccga
     1621 tgcgcgacac actcgccttc gccgtcaacg gcgagtacgc caggatggat gcggtcgtca
     1681 cctccggcga cgaggtggct atcttaccgc ctgtctcggg tggctagatg ttcgcgatag
     1741 tcgaggagcc cctggacatt ggacccttga tcggaaacgc cctgcgacct gagtgcggcg
     1801 cgatagcgac gttcgtcggc accacgagga tcgacgagtc ggagggcgcc tctgtcgagt
     1861 acctcgagta cgaggcatac cgcccgatgg cagacaggaa gcttgaggag atcggggcgg
     1921 agatcatgga ccgctggcac gttgggcacg tcgcgattgt gcatcgtgta ggaagggtag
     1981 atcctggcga ggccagcgtc gccatagtcg tcgccgcgcc gcgccgcgga ccggcgttcg
     2041 aggcgagccg ttacgctatc gagcgcataa aggagatcgt ccccatctgg aagcgcgagg
     2101 tctggtcgga cgggtacgtt tgggtcggca gccaggtcgg cactcgctca ggggctatgt
     2161 gaacaggacg tagacgacga atgctccggc gaaggccagg ctgcccgcga ctatgatcag
     2221 gtaggccgcc actgaaggct cgaagtcgcc agccgagatc ctgcgccggt tgctgaggaa
     2281 ctgaaccgta cctatgatga gcgtgagcgc cccaagcaac gcgagcgcca gcccgaaaag
     2341 ctccgaggcg ctcgtggagc ccaccttcat gcgggcgtca gtcagcgccc ccgcccgttc
     2401 gacgactagc cctatcgaga tcaggctaac ccccgtcctc acccacgaga gcagcgtccg
     2461 ctcgttcgcg aggtgctccc tgatctcggt cccaccctgc tccctgggct cattgtccgc
     2521 ttccatgacc tcagacctcc tcctcacgaa cgctgctctg gccggccaca cttgaggtta
     2581 gtgcaaccat atttttcgga gtttcctgtt cgaatcggca ggaagcgaat tgcgagagtg
     2641 ggcgctgtag acataaaggc aaagctcaaa gcgctgactt caggccggtc tcgcccgtgc
     2701 gacgagcact ggtgggatcc ccacgaggtt ctcccaatcg agcgctcttg tggctggtcg
     2761 tgggccaccc tctggttctg cgaaggaggg ttcctccatg ccatctaccg cgaagccggc
     2821 cctgaagcag gccccgaaca gctctctcaa gggtctgtgg aagtacgggt gcggcacggg
     2881 ctcccctggc atccccccgc ccatcccggg tggcacgtgc aggtaatcgc tcaccttaac
     2941 ggagtacgtc tcaaccaggg caccgtccct gtcctcttcc tccaagacca gcgtgactgc
     3001 gttggagttg aaggccgggt gctgcaccga gaagacgaag cggcctttcg gcttgagaag
     3061 gcggcgcagg ccgagcatca aggggtcgat gaccggcatg tccataagcg ccatgttgca
     3121 gaccgctgca tcgaaacgcc cctctcccaa ggcgagcatc tgctcctcgt cggtcgcgtc
     3181 cgtcaccagg tactccacgg cttcgccgta acccgccttc tcggtgcggg tcctggcgag
     3241 ctcgacgaac ttttcgctaa agtcgatggc cacaaccctc gccccgagct ccgcgagcct
     3301 cctcgagaat accccgttgc cacaggcgac atcgagcacc gtctcgccgg ttcgcagccg
     3361 aagcaaccgt tccgtggcgg gaccgatgag caccctctgg aaggcgttgc cctcgcccat
     3421 cttctcgtcc cagaatgcgg ctttcttctc ccaggcctcg tgcacctggc gcgctagcgc
     3481 cgggtcgctg gtcatagacc tcggtcgctc cttatttgcg ccgtcagtcg cgcccacgcc
     3541 ggtatctccc atcttcttgg cactgggctg gatcttcccc agacaaggca tcatagcgca
     3601 agtacggccc ggcatggcta cgaaagcgga tgccccgtct tcgggtcttg ggcccttcct
     3661 ctgcacttca cacgccagag gagcatcagg ttggagcgcc aaataggcgc ctctttaccc
     3721 ggatatccgc cccggggccg atatctgatc ctcacatctg gggtagagtt tggtccacaa
     3781 aaggcagaca atacaacgta tcctcagggg caaggggaag cgaagggaac aggttgttgg
     3841 atcgacggat cggactagag caggaattct tcctgcttga ggactccggc cttccttcac
     3901 ggcgggccga cgagttcctg gagcgctgcc gggtattgac cgaggaggaa tccggcggtg
     3961 ccgcttgctt cgcccccgag ttcgtgaagg ctctcgtaga gataaacact cctcccgtcc
     4021 acaccctctc cgatctggag cgcgagtatg ccaggaacct gcgtcttgcc ctgcgcacgg
     4081 cgcgctcgct ggggctcagg ctgtatccgc ttggcaccta tccccttccc atggtgcctg
     4141 tcatacgcga cgggctggac tacagtgtcc aggcgaacac ggtcggcccc gagaggttcg
     4201 ccgacgcggg gaggtgcgcc ggcacgcacc tgcacctgga gttgcagaca gggacggtgg
     4261 atgccggagc ggggatctcc cccgcatcgt ccgtcgccgc tcgggacgag cttctcaacc
     4321 tctacaacct tgcgacggcc ctggaccccg ccctggttgc actcacccgc tcgtgtccgt
     4381 acttcgaggg acgaaggtcg gggcttgcca accgcatagt ccactacagg ggaagcgcga
     4441 tcttcgggtg gaagggagtc tacacggatc tgccccaggt aggagccctg ctaccttacg
     4501 ccgacgacgc ggaacacctg atccgacagc agttcacccg ctacaaggcc tggctcacgg
     4561 ccatggacaa ggcaggcgta aagagacatc ttttcgccga ggcaggtggg gatctcctca
     4621 ggccggcctg gaacccggtg cgtctgaacc gtcacgggac gctggaactg cgcggcatgg
     4681 acagcaacta tcccgaggta acccttacgg ccgccgccgt gatcctcggc gccgctgacc
     4741 gtgtcaggcg gaacggcctc gaagtggtac ccgacgacga cgtgctcact ttcgagagca
     4801 ctgaagacgc gctcagggtg cctggcttcg gccgactcgg cggggagcta ttctacgctg
     4861 ccgtaaccag aggggtgaac gacgaaaccg tggccaccta tctggactcg gtcctggatt
     4921 tcgccggggt ggaagaagaa aggctcgcca ggctcagccg tcaacgtcgc accacaggcg
     4981 tctatccgac caccgaggcc gatatcctcg aagaatacgc ctccgatgtc gagaatctgt
     5041 ccgaagacag tggattgcgc atcgtgctga aggcctgtga cgcgctggag gcggaggtct
     5101 ccggcctcgc cggatctccg gcgccataga gaagggcggg ctgacatctc gcaggaaaac
     5161 ctcaccatgg cgggagctgg ccgcaagccc gcacacgtgt catgcttccg cgacgatggc
     5221 atcagaagga ggtggtagag catgagtgag ccaaagcgcg agcttcaggg caaggcggcg
     5281 ctggttaccg gggcgagcag cgggttgggg agggcaaccg cgatttccct cgccagggcc
     5341 ggtgcggatg tcgcactggt agccagaagc ccggaggagc tagagagcgc cagggaggag
     5401 gcatcgaaga ccggcggcag gacgttagcc ctacccgtgg accttgccag agtggacgag
     5461 gcgaagcggg caatcgggga gaccgtcgag gccttcgggc gactagacat cctcatcaac
     5521 gccgccggca ccgacgcgcc aggtacggtg gaggagctgg acgtggagga ctgggaccgg
     5581 actctttcgg tgaacctcag ggcacctttc gtgctctcca gagcggcctt ccctcgcatg
     5641 cgcgaggcgg gaggaggtac tatcgtcaac atttcctcgg tggcggggaa gaagggctgg
     5701 gcgaacgctt cggcctactg cgcctccaag ttcggcctca cgggcttcac cgaggcgttg
     5761 gcggacgaag gtagagagca cggcatcagg gcgatcgtac tgtatcccgg cgcgatggcg
     5821 accaactggg gtgctttctc tcccgaggag cgacggcgga gcgagccgga cgaagcccca
     5881 ccgagccggg tccttcagcc ggagcgcgtc gctgacttca tcgcctggtt tgtcgcatct
     5941 ccgcccgagt tcgtgctgac ggaggggatc atcgttccca tcgaggaagg tttgccgtag
     6001 gacattcaag ggccagggcg gggtccctga cagcgttcta ataacggaag tccaccccgc
     6061 gggtatactc gccgcatggg caaggaccgc gcagcgtcag acatggactt cggggtaaga
     6121 gtcgccgccg tcgtggagcg cgaaggagat ttgctcctcg tgcgccacca gaaaccggac
     6181 agggacccgt actgggtcct gcctggaggc aggctcgagc cgggcgagac gatcccagag
     6241 tgcgcgagtc gcgaggtcgc ggaggagacg ggcctcaccg ccagcttctc cggcgtcctc
     6301 tacctgggcg agttcctgcg ggaaggacgg catacgatcg acgtcgtcgc gcgcgtggaa
     6361 ttggaaggaa acgaagtggc aagcctcggg agcgaccccg aggtcgctcc aggcacagaa
     6421 ccgacactca cagacttacg ctgggtaagc gtcgatgagc tgcgggagat ccagcttcta
     6481 ccagcctcca taaagaagcg gctcctgaga gacgcagata gcgcatggac cccggatgac
     6541 acctatctcg gtaacgtcga tgactgaccc gcagacagct ttgctttcgg ttgtcttgct
     6601 ggacgcagcc gtcgcggggt ttgtgatcgg ggggttctgg atcgcggtct cgctgatgcg
     6661 gagcttcggt aagaagatag gatactccct cgcgccactg ggattctcga agccgagggg
     6721 cggtatcccg tcgggaatcc gcgtcgggat cgccgtggga gtcggcgccg ttctccttag
     6781 catgatcgta aaccccctca gcgttctcgt cctcgaacgg ctcggctacc cgacgaaatc
     6841 gaccgtccag caaccgctta tggaaggact cgtcggctgg gtgcgagaga gtcccggcgt
     6901 ggccgtaccg acgatactcc tcgtcgtcgt gatcttcggt cccgccgtgg aggaactggt
     6961 cttcaggggc atcgtcttca acggcctcta ccggcttggc gccatgatct ctacgaagtc
     7021 agtcaggaaa gagagttcca gagtcccttc cgggaaggcg cttttcgcct tctcagcgct
     7081 cgcgtcgtcc gtgctcttcg ctatgctgca cctggaacct gtccttctgc ccgtcctcct
     7141 tatactcgga atggtcctgt gcgagctatt ccggcggacg gtcagcctgc tgccttcgtt
     7201 cgtcgcccac gcgacgttca actccttcgc cacctcgctc atcatactga acgggctgaa
     7261 cgtcctgaac atacccatct gaagcgaatt gaagaagtcc tgacttcttc aattcgcgct
     7321 ggtcagcact ctacgccctt cgggcttcgc tttctgctcg gcgcctttca gctcgcttcg
     7381 gccttacggc ctcgctttta gtgtttgagt ttttgtcttg gtggtgatct cccgtcttcc
     7441 tgccgggatc gactattgtg agagtccctt taagagtcgc ccgcaaggtc cagaccgtac
     7501 tctcgtctcc tcgcgaactc ctcccatccc gcccgcctga acgcggcggc catcggcgcg
     7561 ttcgcgatat ccgtgtccgc aagcaagggt ctgccgccgg ctcccgatcc tgccgccagg
     7621 agagtcgcag taccggcagc gagcagatcg tcgacgtatc ctcgtcccct catggcaggc
     7681 accaccccga cgtagaagat ggtcaggaaa gcgggtggct cgacgggcat caccagcccg
     7741 acgagatctc catcaggggt gtaggcgagc tgccaccagg agggttcgtg tttcaccctc
     7801 ctggcgtcct cgaagaacac gcgggcggct tgccctgacc ccaggcgttc ccgctcgtcc
     7861 tggatctgcc tgtcgagcgt gccctctgaa accctcgcca ttgcgtcggc gaaggcctcg
     7921 tccccaacct catccagcgc gcggaagtcg agccgcccgg acacagcggg agttttcttg
     7981 ccccgccact cgaagcggat cgtctcgcgt tttagcgcga agccgatgac atccaacagc
     8041 tcggaccgtc tctcctgatg ggactggaac tggggataca tcggcggaga gtcgaggacg
     8101 tgctcgatct cctctgtgcc cagggcacgt gccctatcca gcacgtcgcg aagaaggggc
     8161 acgctgacgg tcaggtcctc ctcttcccag ggcacgtcga gcaggaccag ggcaagcggc
     8221 ctgtctgctc ccgggagagc ccagagggcg acccgcccga ggggtctccc ctcttcttcg
     8281 ataacgaagc accatccggg acgcatggcc ccggcggcga acataccttc aacgtactgc
     8341 ttgacctctt cgttgtgtcc tgtgcttcca gcctcgacga agaggtcgat ctcgtcggca
     8401 tggatcggac gtatgcgcat atttgggagc ctcctgtggc acggttgcgc ggcgggagat
     8461 ctgcttcttc gaaccgggaa agcatgacgt ctggacgcac cgtacacccg aaggagtcag
     8521 ccggcagtat acggcatcca cagggtccca caccacccgg ctctcagcgc ggctgttctc
     8581 cgacgtaccg ggccctgggg cgtatgagcg aatcactctg gtactgctct atggcatggc
     8641 cgatccagcc aacagtcctg cccagggcga agagcgcgag cgccccgcca gcgggaaggc
     8701 caagcacccg cgagactgtc gccagcgcga agtccaccgt gggcagctcg cccatcagtt
     8761 gcagcacttc tccggccaca gcctcatgaa gtgccacggc gggaccctcc ggatacgcct
     8821 cggcggtcag gcgtagtagc gctgcgccgc gcgggtcccc ctcggggtag agcgagtgcc
     8881 cgaaccctgg gatgcgctcc ccgcgcctta gacggccaga gataaccgcc cgcgcgtctc
     8941 cggtgtcctc cacctcccgc aggaaggcct cgacgagttc gatctcccct ccgtgcttga
     9001 cgcctccgag cgccgcgagc ccggccagaa cgacggcata aggtgtggcc tcggatgaag
     9061 ccacgcaccg ggcggcaaat gtcgagaccg gcaactcgtg gtccaca
//
LOCUS       DATAIR010000694        12347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_348789, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000694 DATAIR010000000
VERSION     DATAIR010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348789"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHM69_16360"
     CDS             complement(<1..648)
                     /locus_tag="VHM69_16360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2742019.1"
                     /translation="MPEVHGALERSGEPGYRLGQAYAALTRSLVRDWEEATSLPQELR
                     GRLNEEAPAAVLDLRRTWLATDGTRKYLFYTHDGHAIETVMIPEKGRHTVCISTQVGC
                     AMGCKFCATGLLGIKRNLKAREIAEQVFVAARDIVPERVSNVVVMGMGEPMLNYNATL
                     AALKVLNDADGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEDERRE"
     gene            843..1991
                     /locus_tag="VHM69_16365"
     CDS             843..1991
                     /locus_tag="VHM69_16365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX2742020.1"
                     /translation="MSALDLMIALFVILSVLRGARTGFLAGVFSLVGVVVGASVGSRI
                     AQSLMPDDGNPIYGAGITLGSILAFAVLGEVVARTIGSSLRNRLSSPASEALDGLGGA
                     ALGFALSLVLVWAVGAFALQSPPLAGLQPAVQESRILDTLNERMPSGLLTRAVADLQP
                     LPQIQGPEPDVAAPEGSIVRDPDVQTASASTLRITGIACGYGVEGSGWAAGRDLVVTN
                     AHVVAGETATRVQVGGTGRPLPAQVVVFSEKNDIAVLRVNGLGLTPLRLSAPRPGEAS
                     AVIGFPENGPLDIEPARTGTTRRVISSDAYNHGPVERIVTSFRVYVRPGNSGGPAVNA
                     DGQVVSTIFASRTDSDNAGYGVPSRIVRHYLQIATARYSPVSTGGCAN"
     gene            complement(2090..2266)
                     /locus_tag="VHM69_16370"
     CDS             complement(2090..2266)
                     /locus_tag="VHM69_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742021.1"
                     /translation="MLTLESAVTAIEEAATAVAHEVERDRLRESSLARLSTVEAELNR
                     SRLALEKIIREETA"
     gene            complement(2292..2522)
                     /locus_tag="VHM69_16375"
     CDS             complement(2292..2522)
                     /locus_tag="VHM69_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742022.1"
                     /translation="MRSPILAAVLSLIVAGLGQIYNGQIGKGVIFIVIQLINGALTAV
                     LIGWVLLPIVGLWAMIDAYLTAKRNNERYGFM"
     gene            2711..3325
                     /locus_tag="VHM69_16380"
     CDS             2711..3325
                     /locus_tag="VHM69_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2742023.1"
                     /translation="MSEEPDRRAQILEAAFEEFSAKGFKGATIKSIARTARLQSPALI
                     YWYFPDKEALFREVLESRIPVLRTVRDPTGLLDLPPEKVLPTIARGYLSTFDDPAAQR
                     MIRLMVGEAMRRPEIAEIFGNTVIRRVLGFLKSYMARQIELGRLRPHDVRASSRAFMG
                     MLLPQAGGKVFFHVLREDGLTDEEHIQAAVGIFLRGLEGKEEKG"
     gene            3322..4173
                     /locus_tag="VHM69_16385"
     CDS             3322..4173
                     /locus_tag="VHM69_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742024.1"
                     /translation="MNAIEVSGLSKRYGRVEALWDVDLSVRRGEVFGLVGPNGAGKTT
                     LIKGLVGALRPSGGEARVMGLNPLKDRAELRQHIGYMPQSPALYEDLSARGNIGFFGA
                     AHRTPDLRKKVDEVLRFTELSGRANDPMHTFSGGMKRRVSLACALVHGPEVIFLDEPT
                     AAVDPQLRSRFWSTFRELAEDGATLFISTHLMDEAMLCDRIAILRKGRVIAADTPRAI
                     LEMGRTHLVVKYDGTEEETTIGGHPDDLAAALRRYGMRPEVKAVDVRSDSLETVVLSL
                     IEKDEVA"
     gene            4173..4910
                     /locus_tag="VHM69_16390"
     CDS             4173..4910
                     /locus_tag="VHM69_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742025.1"
                     /translation="MRRMFIVAGRVVRQLLRNPRFLVLSVAAPLIIVYFLKIFFDTMP
                     PTFDVARYAVPVAAFLVHFLAFLLCAISLVQERTAGTMERMFINGFRRTEIIAGYVLG
                     FLGLATFQAVAGLTEAIWLFELDYDEETLVTLFVVVWLLAIASVMLGIFVSTFARHEG
                     QVFPFIPLIIVPSVFLSGLLIDVDELPGWAEWLGHAFPLFWANDVIQEIIGSGWTFED
                     IQSSLAVLVGYSVVLLLLASLTLREVE"
     gene            4932..7628
                     /locus_tag="VHM69_16395"
     CDS             4932..7628
                     /locus_tag="VHM69_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2742026.1"
                     /translation="MNEPEKSPLDLIFRFEEIDRTALPVAGGKAANLGELARAGLPVP
                     EGFCVTTAAYEVVAADAGLEPTLGALADTRPEDTERLADLAAEARSRLSEASVPEALT
                     RAISLAYEELGEGEPVAVRSSATAEDLPYASFAGQQDTYLNVVGVESVLDAVRRCWAS
                     LWTDRAVSYRATNGIDPHGVRLAVAVQRMVDASVAGVLFTANPVTGKRRQAVIDASPG
                     LGEAVVSGMVNPDHFVVDTQKGTIVERRLGDKRVAVRAAAGSGTERVEMAAGEDEASL
                     SDGQILTLAKLGARVEDHYGSPQDTEWAIDVDGKIWLLQARPITTLFPLPANVPTDDE
                     DLRVYFSFSVAQGVYRPLTPMGFQAFRLIFTSLATMAGRPPRNPYQGPAFVIEGAGRI
                     FADITPVLRSKQGRRFFNLIMRNMEARTVPIVRRLVEDPRLAPVQTSWRPVLRAVLPI
                     FVRGRIPLRIIQALARPEKARERAARVVARLRANDLPRNASAAERLSAAERMLRDGPP
                     GILPAVPPAFITGVLSNALAGKILGNLATDDERRVAMRALPHNPTTEMDLALWDLARE
                     ASAVPDTARTLGETPPERLAEDYRRGSLPPNLQAGLEDFLRLYGHRGVAEIDLGLPRW
                     SEDPTYILGVLSNYLRLDDPQAAPDVQFGRAAREAEEMVAELTRRARRKGRLRGALVG
                     FLLDRARELSGLREMPKFCIVLLMARARGLLWKVGDELAEAGRLERAGDIFFLSVPEA
                     WAVLSGEDLRPLVRGRRAVYEQEVGRKHVPRLLLSDGTEPTAETNGAAEGVLLGTPAS
                     GGVVTQKARVILDPSGARLEPGEILVAPSTDPGWTPLFLTAGGLVMEMGGPMSHGAIV
                     AREYGIPAVVGVPDATERINTGQRITVDGAAGEITPEADGAT"
     gene            complement(7695..8636)
                     /locus_tag="VHM69_16400"
     CDS             complement(7695..8636)
                     /locus_tag="VHM69_16400"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX2742027.1"
                     /translation="MREQTTRQGVRSTESPGTYADGAAFVEGRFVPVAEARVPILDWG
                     FLRSDATYDVAHVWKGSFFRLNDHLDRFERSMNHLRLSPPYGRDEIQDILVECVRLSG
                     LRDAYAEVICTRGIPKPGSRDPRECENRFYAFVVPFVWIAYPEKQERGLHATIGRGQR
                     IAPESVDPTVKNYHWLDLETGLLEAYERGGETVILVDAKDNIVEGPGFNVFAVKDNEI
                     STPDRGVLEGVTRKTVIELAAEQDIPLEARSVPADELRRADEVFLSSTAGGIIPVTTV
                     DGEPVGNGEPGHLTRRLRDAYWALHGDPRFSLPVSYG"
     gene            8829..9605
                     /locus_tag="VHM69_16405"
     CDS             8829..9605
                     /locus_tag="VHM69_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742028.1"
                     /translation="MMAVFEHGTSRIYYEVHGSGDPVLLLPGFAVSMTELSALREALA
                     ARYRVISADLPGSGRSGPQPREYTPAYFEEDARSFAALLRHLETGPGHLVGYSDGGEV
                     SMLMAAMTPDLVRSVATWGAAGTIPEEQLPLLDAFYDVVDNPIEPLQGFSEQLKAEYG
                     EANARAMTRSFMNAIRAIIEGGGGDISLSRAGGITCPVLLIVGEHDFFVSPALVAQVA
                     DRIPSAEVIRVEGAGHGVHEDRPEWLARTLIDWLTEHQIP"
     gene            complement(9764..10645)
                     /locus_tag="VHM69_16410"
     CDS             complement(9764..10645)
                     /locus_tag="VHM69_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742029.1"
                     /translation="MLKKTARIATRIARATTTVVGLAIMLALVFGVSTTAIGATGSGF
                     ILGKGNTAGKQTSLVSTVVDITQSALLIKNGKGGSALELRVVDPDTTDPALKDVAPMK
                     VDSQAKVTNLNADEIDGKDSTDFYAAGSKVNAAHHADTAYQAQDANNANTLNGKPSNY
                     FAPRAVEPWHVIGSASEPGFQNGWTNFDPNGFFSTAAFYKDPYGVVHLKGVIKGGTVS
                     HGSSGVIFMLPYGYGSEQDEVHAVLSNEGLGRVTIERGSSVSDGRTVSYVHAAPPSNS
                     AWVSLDGITFRAAGPLN"
     gene            10910..>12347
                     /locus_tag="VHM69_16415"
     CDS             10910..>12347
                     /locus_tag="VHM69_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2742030.1"
                     /translation="MYKEIYVPVDNSDYSNQACVIGVDVARQFGGRVAGCHAYAAKMH
                     DVRFRQMESGLPEEFRDEDEMKRQRKIHDQLITKGLEIITDSYIDVLEPLCEKYDVEL
                     VRRSLEGKNFKVIVEDVNANDFDLVVIGAMGMAAVKDTVLGTVTERVVRRLQKADTLI
                     VKDLDRSPFEHIVVAVDGSAKSLGGLKRAIELAREFGGTVEAISVFDPYFHYAMFHSI
                     AGVLSSKAQKVFRFKEQEKLHEEIIDSGLAKIYTAHLEIAKKIAEDEGVDLKTTLLAG
                     KPFEQTMKYVNEVNPTMVVMGRIGYHSDDEMDIGGNTENMMRYLPANVLITNYEYQAP
                     DILTAEEHMTWTKEALERLDRIPGFVVGMATGAIIRYAIERGYTVITTGVIDEVIKNI
                     LPPGAMESMRAIGDQMRAAEAMGEEQPIDSLFKDFDERTADKNAAKAKANGNGSGNGS
                     GNGSANGTSNGNGNGHVTKEEIFEAGRGG"
BASE COUNT         2254 a         3957 c         3848 g         2288 t
ORIGIN      
        1 ctcgcgccgc tcgtcctcga agggggtatg caacgatatc gcaaggtgga actgctcggg
       61 ctcgtccgcg aaacgccgga tcttgtccac caacccactc gtcgagaccg cgatgtgcct
      121 cgccgccaac ccgaacccgt cagcgtcgtt caagaccttt agcgccgcga gggtcgcgtt
      181 gtagttgagc atcggttcgc ccatccccat gacgacgacg ttggatactc tctcggggac
      241 gatatccctg gccgcgacga agacctgctc cgcgatctcg cgcgctttca ggttgcgctt
      301 gatgccgagc aggccggttg cgcagaactt gcatcccatc gcacacccga cctgtgtcga
      361 gatgcagacg gtgtgcctgc ccttctcggg aatcataacc gtctctattg cgtgcccgtc
      421 gtgggtatag aacaggtact tcctggtccc gtccgtggcc agccaggtac ggcgaaggtc
      481 cagtaccgcc gccggagcct cctcgttgag cctcccgcgc aattcttgcg gcaggctcgt
      541 ggcttcctcc caatcgcgca ccagagagcg tgtcagcgcc gcgtatgcct gcccgagacg
      601 gtagcccggc tcaccgctcc tttcgagcgc cccatgtact tccggcatga actttgcggc
      661 tctcatcgac cgtggattat atcaagcacg ctggcgcagc cagcttgtca gcacgcttcg
      721 tccgctcggt acgtgcatga gcttccctcg aaggttctct ggtgcgtctc gggcgacgcc
      781 tacttcccgg ctgaaatgct gaaagccgcg tagcggcgcg ctgacaatgt agaatcttac
      841 ggatgtcggc gctcgatctc atgatagccc tcttcgtgat cctgtccgtg ctgcgcggcg
      901 cgaggacggg ttttcttgcc ggcgtcttct cgctggtcgg cgtcgtcgta ggcgcctccg
      961 tgggatccag gatcgcgcag tccctgatgc cggatgacgg gaaccctatc tacggggcgg
     1021 ggataacgct cgggagcatc ctggccttcg cggtgctcgg ggaggtcgtc gcccgtacta
     1081 tagggagctc gctgaggaac cgtctctcca gcccggcctc cgaagcgctg gacggactgg
     1141 gtggggcggc gctcgggttc gccctctccc tcgtgctggt gtgggccgtc ggcgccttcg
     1201 ccctccagtc cccacccctc gcgggactgc aacccgccgt gcaggagtcc aggatcctgg
     1261 acactctcaa cgagcggatg ccctccgggc tcctcacccg ggccgtcgcc gatctacagc
     1321 cgctgcccca gatccagggc ccagaaccag acgtggccgc cccggaaggg agcattgtca
     1381 gagatcccga cgttcagact gcgagcgcga gtacgctgcg cattaccggc atagcatgcg
     1441 gctacggcgt ggaggggtcg ggctgggccg cgggccggga tctggtcgtc accaacgccc
     1501 acgttgtcgc gggggagact gcgacgcggg tgcaggtggg aggcacggga aggcccctgc
     1561 ccgcccaggt cgtcgtcttc agcgagaaga acgacatagc cgtcctgcgc gtaaacggcc
     1621 tcggcctgac ccctctgcgt ctctccgccc cccggccggg cgaggcatca gccgtgatcg
     1681 gtttccccga gaacggcccg ctcgacatcg agcccgcccg caccggcacg acgcgccgcg
     1741 tgatctccag cgatgcctat aaccacggcc ccgtggagcg tatcgttacc agcttccgcg
     1801 tctacgtcag gccaggcaac tccggcggtc ccgcagtcaa cgcagatgga caggtcgtct
     1861 ccaccatctt cgccagccgt accgactccg acaacgccgg ctacggcgtc ccctcccgga
     1921 tagtccgcca ttatctccag atcgccaccg cccgctactc tcccgtcagc accggtggat
     1981 gtgcgaatta ggcatcaggc tacaggcttc aggaagtacc tctggccagg tctgtgccac
     2041 gcgggcaacg ttgcccacgg ccttacctga cggctgatac ctgaagcctt taagctgtct
     2101 cttcgcggat gatcttctcc agcgcgagcc ggctcctgtt gagttcggcc tcgaccgtgg
     2161 agaggcgggc gagggagctc tcgcgcaacc tgtcgcgctc gacctcgtgg gcaacggcgg
     2221 tggccgcctc ctctatggcg gtgacggcgc tttccagcgt cagcaactct cgctcatcca
     2281 cgggacggat attacatgaa gccgtacctc tcgttgttgc gcttcgcggt caggtacgcg
     2341 tcgatcatgg cccataggcc aacgatcgga agcagtaccc agccgatcaa aactgccgtc
     2401 agcgcaccgt tgatgagctg tatgactatg aagatgacac ccttgcctat ctggccgttg
     2461 tagatctgac ctagcccagc aacgatcagg ctgaggacgg cggccagtat gggactgcgc
     2521 atggacttct cctcgctgtc gactacttca tctattcagg tatacgtccg aaatcgctac
     2581 tcgtttccgc gcctgcgctc gtcctcgccc ggctccacgt cgagcctcgt aaacgctacg
     2641 cacttttctc ttgactgaac gttctatcaa gtgtaaagtg attgattaaa cgatcaataa
     2701 aggggcaagg atgtcggagg agccggacag gcgggcgcag atcctggagg cggcattcga
     2761 ggagttctct gcgaagggct tcaagggggc cacgatcaag agcatcgcgc gcacggcgcg
     2821 actacagtct ccggcgctca tctactggta cttccctgac aaggaagcgc ttttcaggga
     2881 ggtactcgag tcccgcatcc ccgttttgcg cacggtgcgg gatcccacgg gcctgctcga
     2941 ccttccgccg gagaaggtgt taccgacaat agcgcgggga tacctctcga ccttcgacga
     3001 tcccgcggcc cagaggatga tacgtctgat ggtaggagag gccatgaggc gacctgagat
     3061 cgccgagatc ttcggcaaca cggtaataag gcgggtgctc ggcttcctca agagttacat
     3121 ggcgcgccag atcgagctcg gaaggttgcg cccgcacgac gtgcgggcga gctcacgggc
     3181 cttcatgggg atgcttctcc cgcaggccgg gggcaaggtg ttctttcatg tcctgaggga
     3241 agacggtctg accgacgagg agcatataca ggcggcggtc gggatcttct tgcgaggtct
     3301 cgaagggaag gaggagaagg ggtgaacgcc atagaagtct ccgggttgtc caagaggtat
     3361 ggtcgggtcg aagccctgtg ggatgttgac ctgtcggtac gaagaggcga ggtattcggc
     3421 ctggtagggc cgaacggggc cggcaagacc accctcatca aaggcctcgt cggggcgctg
     3481 cgcccatcag gcggtgaggc gcgggtgatg ggtttgaacc cgctcaaaga ccgggccgag
     3541 ctgaggcaac acatcgggta catgccccag tcgcccgcgc tctacgagga cctctccgcc
     3601 aggggcaaca tcggcttctt cggcgcggct caccgcacgc ccgaccttcg gaagaaagta
     3661 gacgaggtgc tacggttcac cgagctgtcg gggcgggcga acgaccccat gcacaccttc
     3721 tcaggcggga tgaagcggag ggtctcgctc gcctgcgccc tcgttcacgg gcccgaggtc
     3781 atcttcctcg acgagccgac cgccgccgtc gacccgcagc tcaggagccg tttctggagt
     3841 actttccggg agcttgcaga ggatggcgcg acgctgttca tcagcacgca cctcatggac
     3901 gaggcgatgc tctgcgacag gatcgcgata ctcaggaagg gcagggtcat cgccgcagat
     3961 acccctcgcg ccatcctcga aatgggcaga actcatctcg tggtgaagta cgacggcacc
     4021 gaggaggaga cgaccatcgg cgggcatccc gatgacctgg ccgcagcgct tcgacgctac
     4081 ggtatgcggc ccgaagtcaa ggccgtagac gtgcggagcg actcgctcga gacggtggtc
     4141 ctgtccctga tcgagaagga cgaggtggcg taatgcgccg gatgtttatc gtcgcgggga
     4201 gggtcgtcag gcagctactg aggaacccga ggttcctcgt cctctccgtg gccgcgccgc
     4261 tcataatcgt gtatttcctc aagatcttct tcgataccat gcctccaact ttcgacgtgg
     4321 cgaggtacgc cgtgcccgtg gcggccttcc tagtccattt cctggccttc ttgctctgcg
     4381 ccatctcgct cgtacaggag cgcacggccg gcaccatgga gaggatgttc ataaacggat
     4441 tcaggcgcac ggagataatc gcggggtacg ttctcggctt cctcgggctc gcgaccttcc
     4501 aggcggtcgc cgggttgacc gaggcgatct ggctcttcga gctggattac gacgaggaga
     4561 cgctggtgac gctgttcgtc gtggtctggc tccttgccat agcctccgtg atgctcggta
     4621 tctttgtctc cacgttcgcc cgccacgagg gacaggtctt tccattcata ccgctcatca
     4681 tagttccatc cgtattcctc tcggggctgc tcattgacgt ggacgagcta cctggctggg
     4741 cggagtggct cggacacgcc ttcccgctgt tctgggccaa cgatgtgatc caggagatca
     4801 tcgggtcggg ctggacgttc gaggacatac aaagcagcct cgccgttctc gtcggctaca
     4861 gcgtggtcct gctcctgctc gcctcgctca cgctgcggga ggtcgagtag ccgggcagaa
     4921 aggagaaact gatgaacgag ccggagaaaa gtccccttga cctgatattt cgattcgagg
     4981 agatagaccg caccgcacta cccgtggcag gcggcaaggc ggcgaacctc ggtgagctgg
     5041 cgcgtgccgg gctccctgtg ccagaagggt tctgcgtcac cacggcggcc tacgaggtcg
     5101 tggcggcaga cgccggactg gaacccacgc ttggtgccct ggccgatacg cgtcccgagg
     5161 acacggagag actcgccgac ctcgccgcgg aggcgagatc caggttatct gaagcaagcg
     5221 tacccgaggc tctcacgcgt gcgatctcgc ttgcttatga ggaattggga gaaggagagc
     5281 ccgtcgccgt ccgctcatcg gcgacggcgg aggacctgcc ctacgcgagc ttcgcgggcc
     5341 agcaggatac gtacttgaac gtcgtcggcg tcgagtcggt gctcgacgcg gtgcggcgct
     5401 gctgggcgtc gctgtggacc gatcgcgccg tcagctacag ggctactaac gggatcgacc
     5461 cgcacggcgt gcgcctcgcc gtcgccgtac agcggatggt cgatgcgtcg gtcgcaggcg
     5521 tattgttcac cgccaacccg gtcacaggga agcgccgcca ggccgttatc gacgcaagtc
     5581 cgggtctcgg cgaggccgtc gtctcgggca tggtcaaccc agatcacttc gtcgtggaca
     5641 cgcagaaggg cacgatcgtc gagcgcaggc tcggcgacaa gcgggttgct gtacgggccg
     5701 ccgctggtag cggcaccgag cgggtcgaga tggctgcagg cgaagacgaa gcctccctct
     5761 ctgacggtca gatcctgacc ctcgcgaagc tgggtgctcg ggtcgaggac cattacgggt
     5821 cgccgcagga cacggagtgg gctatcgacg ttgatgggaa gatctggctc ttgcaggccc
     5881 gcccgatcac gacgctgttt cctctgccgg cgaacgtccc gacggacgat gaagacctgc
     5941 gcgtctactt ctcattcagc gtcgcccagg gcgtctaccg cccgctaacg ccgatggggt
     6001 ttcaggcgtt ccgtctcatt ttcacttcgc tggcgacgat ggcgggccgt ccgccgcgca
     6061 acccctacca gggacctgcc ttcgtgatcg aaggcgccgg acgcatcttc gctgacataa
     6121 cgcccgtttt acgcagcaag caagggcgca ggttcttcaa cctgatcatg cgtaacatgg
     6181 aagcgcgtac cgtgcccatc gtccgtcgtc tcgtcgaaga tccacgcctc gcacccgtgc
     6241 agacatcctg gcggcctgtg ttgcgggccg tgctccccat attcgtgcgg ggccgcatcc
     6301 cgctccgcat catccaggcg ctcgcgcgtc cggagaaggc gagggagcgc gcggcgcgtg
     6361 tggtagcacg gctgcgcgca aacgacttgc ccaggaacgc cagcgctgcg gaacgtctct
     6421 ccgcagccga gcgcatgctg cgcgacggcc cgccggggat cctgcccgcc gtgcctccgg
     6481 ccttcatcac gggcgtgctg tcgaacgccc tcgccggcaa gatcctcggc aacctcgcca
     6541 ccgacgacga gcggcgggtg gccatgcgcg ctctcccgca caaccccacc acggagatgg
     6601 accttgcgct ctgggatctc gccagagagg ctagcgcagt ccctgacacc gccaggacgt
     6661 tgggcgaaac accgcctgag cgtctggctg aggactatcg tagaggttcc cttcccccga
     6721 atctccaggc cggcctggaa gatttccttc gtctctacgg gcatcgcggg gtggcggaga
     6781 tcgacctcgg tctgccacgc tggtctgagg acccgacgta catcctcggc gtgctctcaa
     6841 attacctgcg tctcgacgac ccacaggcgg ccccggacgt ccagttcggg cgggcggcgc
     6901 gcgaggccga ggagatggtc gccgaactca cccgacgcgc caggcgcaag ggccgtcttc
     6961 gcggggcgct ggtcgggttt ctgctcgacc gcgccagaga gctttcgggg ctgcgcgaga
     7021 tgcccaagtt ctgcatcgtc ctcctcatgg cgcgtgccag ggggcttcta tggaaggtcg
     7081 gtgatgaact ggctgaagcc gggcgtctgg agagggcagg cgacatcttc ttcctctccg
     7141 tacccgaggc gtgggccgtg ttgtcgggtg aagacctccg ccccctcgtc agagggcgcc
     7201 gcgcggttta cgagcaggag gtcgggcgca agcacgtacc gcgactccta ctctccgacg
     7261 gcaccgagcc aaccgccgag acgaacggtg cggctgaggg tgtccttctt gggacgcccg
     7321 cttcaggcgg cgtcgtgacg cagaaggcgc gtgtgatcct cgacccaagc ggagcaaggc
     7381 tggagccagg tgagatcctg gtcgcgccat cgaccgatcc tggttggaca cccctcttcc
     7441 tcacggccgg cggcctggtg atggagatgg gaggaccgat gtcccacggc gccatcgtcg
     7501 cccgcgagta cggcatcccc gccgtcgtcg gtgtccctga cgccacggaa cgaatcaaca
     7561 caggacagcg gatcaccgtg gacggggccg caggcgagat cacacccgaa gccgacggag
     7621 caacttgagc caggtccgca attgcagaag aatagcgtcg tcagcgctgc tcagatagca
     7681 tcgcgcacgg cgtctcagcc gtagctgacg ggtagggaga agcgcggatc gccgtgcagt
     7741 gcccaatagg cgtcacgtag acgccgggtc aggtgacccg gctccccgtt tccaacaggc
     7801 tcgccgtcga ccgtcgtaac agggatgatc ccgcccgcgg tgctcgatag aaagacctcg
     7861 tctgcccgcc tgagctcgtc cgcagggacg gagcgggctt cgagcgggat gtcctgttct
     7921 gcggcgagct cgatgacggt cttccgggtg acgccttcca gtacgcccct gtcgggggtg
     7981 gagatttcat tgtccttcac ggcgaagacg ttgaagccag gtccctcgac gatgttgtct
     8041 ttggcatcga ccaggatcac cgtctcccca ccgcgttcgt aggcttcgag aagtcctgtc
     8101 tcgaggtcga gccagtggta attcttcacc gtcgggtcca cagactcggg cgctatgcgc
     8161 tgcccgcggc cgatggttgc gtgcagcccc cgctcctgtt tctcggggta tgcgatccac
     8221 acaaaaggca cgacgaaagc gtagaagcgg ttctcgcatt ctcgcgggtc ccgcgaaccc
     8281 ggcttgggga tcccacgcgt gcagatgacc tcggcgtagg cgtcgcgcag cccgctcagg
     8341 cgcacgcact cgacgaggat gtcctggatc tcatcccgcc catacggcgg actcagtcgc
     8401 aaatggttca tcgaccgctc gaaacggtcg aggtggtcat tcaatcggaa gaagctcccc
     8461 ttccaaacgt gggccacgtc gtaggtcgcg tccgagcgca ggaaccccca gtccaggatc
     8521 gggacccgcg cctccgcgac cggcacgaac cgtccttcga cgaaagcagc gccgtccgcg
     8581 taagttccag gactctcggt cgatctgacg ccttgccttg tagtctgttc gcgcatctct
     8641 acctcacccc atccaccttg tttgggaaga ctgcgccgcg agagtcaaat ctgtttgcca
     8701 aataggtacg acagaaagct cgcgaacaat acagtccaac cctcatcgct atcaaacaca
     8761 tccgaaacac tgcttgctag tatatgatcg tccaccgtcc gggactattt cgctgtccca
     8821 ggaggcttat gatggcagtg ttcgagcacg gaacgtcccg catctattac gaagtgcacg
     8881 gcagcggcga ccctgtcctc ttgctgcccg gattcgccgt gagcatgacc gagctttcgg
     8941 ccttgaggga agccctggca gccagataca gggtgatctc cgccgacctg ccaggttctg
     9001 gccgctcggg accgcagcca cgtgagtaca cgcccgccta cttcgaggaa gacgcgcgtt
     9061 cgttcgcggc acttctacgg catctggaga ccggcccagg tcacctggtc ggctacagtg
     9121 atgggggcga ggtctccatg ctcatggcag ccatgacgcc ggatctcgtg cgctctgtgg
     9181 cgacctgggg cgctgcgggt acgatacccg aggaacagct tcctctcctc gatgccttct
     9241 acgacgtggt cgacaacccc atcgagccgc ttcagggttt cagcgagcaa ctcaaagccg
     9301 agtatgggga ggccaacgcc agggccatga cgcgaagttt catgaacgcc atccgcgcca
     9361 taatagaggg gggtggtggc gacatcagcc tctccagagc aggggggatc acatgccccg
     9421 tgctacttat cgtgggcgag cacgacttct tcgtctcgcc tgcgctagtc gcccaggtgg
     9481 ctgaccgcat acccagcgcc gaggtaataa gagtcgaggg ggccggacac ggcgtgcacg
     9541 aagaccgccc cgaatggctc gcccgaaccc tgatcgactg gctcacagag catcagatac
     9601 cttagaatcc aaaccctccg cacccttccg acagtcctcc ctggtagcct ggcgacgccg
     9661 gaagctagat ccggccatat tctgaggtct ggaacgcctt ctcaacaggc gaataggccg
     9721 gagtctgaag gaccccggcc cacgaaggtc agacgctact tcactagttc agagggccgg
     9781 ctgcgcggaa cgttatcccg tcgagtgaga cccaggctga gttgctcggc ggcgcggcgt
     9841 gtacgtaaga cacggtccgc ccatcgctga cggagcttcc cctctcgatg gtcaccctgc
     9901 ccaatccttc attcgagagc accgcatgta cctcgtcctg ctcggagccg taaccgtagg
     9961 gcagcatgaa gattacgcca gaactgccgt gggaaacggt tccacccttg atcacgccct
    10021 tgaggtgcac cacaccgtag gggtccttat agaaggccgc cgtgctgaaa aacccgtttg
    10081 gatcaaagtt cgtccagccg ttctggaagc cgggctcgct cgccgacccg atcacgtgcc
    10141 acggctcaac agccctgggg gcgaagtagt tcgacggttt gccgttcaag gtgttggcat
    10201 tgttggcgtc ctgggcttga taggccgtgt cggcgtggtg ggcggcgttg accttgctgc
    10261 cagcggcgta gaaatccgtc gagtccttgc cgtcgatctc gtctgcgttg aggttggtga
    10321 ccttcgcctg ggagtcaacc ttcatcgggg cgacgtcttt cagggctggg tctgtggtgt
    10381 cggggtcgac cactcttagt tcgagggcgc tgccaccctt gccgttcttg atcagcagcg
    10441 cgctctgggt gatgtccacg acggtactga cgagagaggt ctgcttgcct gcggtgttgc
    10501 ccttcccgag aatgaatcca ctcccggtcg ccccgatggc cgtcgtcgac accccgaaaa
    10561 ccagggcaag catgatcgcc aaccccacaa ccgtcgttgt agccctggcg attctagtcg
    10621 ctatcctggc agtcttcttc aacatggtcg tctctcctgt ctgtggctgc tcgactgtac
    10681 tcagtctgcg cccggggaag ggtcccaacc atccacactg ctcctacatc gttcccacat
    10741 atgtcccaca gttcattaac cggtcggatt gatcccgttc gtgtgaacgc ttttatttgc
    10801 ggtgtatccg gctaaacggt aagctacgtt ggcgcggaga aggagcgtcc tcacgcgtta
    10861 tgaaacaacg aacttctcgg cgtcctgcaa agctaggaga acggggtata tgtacaaaga
    10921 gatctacgtc ccggtagaca actcggacta ctccaaccag gcgtgcgtca tcggagtaga
    10981 cgtcgcgcgg cagttcgggg gaagggtcgc gggttgccac gcctacgcag ccaagatgca
    11041 cgacgtccgc ttccgccaga tggagagcgg tctcccggag gagttccgcg acgaggacga
    11101 gatgaagcgg caacgcaaga tccacgacca gctgataact aaaggcctgg aaattattac
    11161 cgactcgtat atcgacgtgc tcgaacctct gtgcgagaag tacgacgtcg aactggtccg
    11221 ccgctccctc gaaggcaaga acttcaaggt catagtcgag gacgtgaacg cgaacgactt
    11281 cgacctcgtc gtgatcggcg ctatgggcat ggccgccgtc aaggacaccg tcctcggcac
    11341 cgtaaccgag cgcgtcgtca ggcgtcttca gaaggctgac accctgatcg tcaaggacct
    11401 cgaccgctcg cccttcgagc acatcgtcgt cgccgtggac ggctcggcca agtcactcgg
    11461 cggcctcaag agggcgatag agctggcgcg tgagttcggc ggaacggtcg aggccataag
    11521 cgtcttcgat ccctacttcc actacgcgat gttccactcc atagccggtg tcctctccag
    11581 caaggcgcag aaagtcttcc ggttcaagga gcaggagaag ctgcacgagg agatcatcga
    11641 ctcgggattg gccaagatct acaccgcgca cctggagatc gccaagaaga tcgccgaaga
    11701 tgaaggcgtc gatctgaaga cgacgctcct tgcgggcaag cctttcgagc agacgatgaa
    11761 gtacgtcaac gaggtcaatc cgacgatggt cgtgatgggc cgcatcggct accactccga
    11821 cgacgagatg gacatcggcg gcaacaccga gaacatgatg cgctacctgc cggcgaacgt
    11881 cctcataacc aactacgagt accaggctcc ggacatcctc accgccgagg agcacatgac
    11941 ctggaccaag gaggccctgg agcggctcga ccgcatcccc ggcttcgtcg tcgggatggc
    12001 gactggtgcg atcatccgct acgccatcga gcgtggctat acggtcatca ccaccggcgt
    12061 catagacgag gtcataaaga acatcctccc ccccggcgcg atggagtcca tgcgcgccat
    12121 cggcgaccag atgcgcgccg ccgaagcgat gggcgaagag cagcccatag attccctctt
    12181 caaggacttc gacgagcgca ccgccgacaa gaacgccgcg aaagcgaagg ccaacggcaa
    12241 cggctccgga aacggctccg gaaacggctc cgccaacggt acgtccaacg gaaacggcaa
    12301 cgggcacgtc acgaaagagg agatcttcga ggcgggccgc ggaggct
//
LOCUS       DATAIR010000695         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_69514, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000695 DATAIR010000000
VERSION     DATAIR010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69514"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VHM69_16420"
     CDS             <1..226
                     /locus_tag="VHM69_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX2742031.1"
                     /translation="SPDKKWSDLTAREKAPFVIRGVVQFTLLVAALVDIYRRPAEEIR
                     GSKWLWSAAAFVNFMGIGPIVYFALGRKRT"
     gene            257..970
                     /locus_tag="VHM69_16425"
     CDS             257..970
                     /locus_tag="VHM69_16425"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEX2742032.1"
                     /translation="MRQTVLLLVAALALVFFVGACQTQAVGDLQRETNTIQPETAEAV
                     SAHLMMGAGELKVSGAADALMEGDFTYNVADWKPKVDYDVLGDTGELSVAQGSGEGIP
                     FAGDARNEWDLRFNDQVPTDLVVDMGAGESDLDLDSLNLTGLTLHMGAGKTTVDLAGD
                     YSRSFDASIEGGVGEATVLLPSEVGVRVTAEGGLGKINAEGLNRDGEAYVNDVYGDSD
                     VNLEVVVRGGVGEINLEVV"
     gene            1266..1961
                     /locus_tag="VHM69_16430"
     CDS             1266..1961
                     /locus_tag="VHM69_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2742033.1"
                     /translation="MAVDVSLKEYTKLQEQECRALLQLLDEMGIATADRVYGPGDAIY
                     NEGEYGDALYVLTSGVLKLFRPYSGSKEATLRLLKPWDIFGHLAFAGETRQRAYAEAV
                     TECRVTKVPKVFVERAVRQEPRVAFKLMTLLELRLVQYEELVQCLLPRETEVRLANLL
                     PLLAQKFGEYRNGSVTIDLRLTHQDLAAMVASTRESVTKVLNELRNRGVIEVDSGRIT
                     LKDTRALTILSRP"
     gene            2067..2888
                     /gene="uppP"
                     /locus_tag="VHM69_16435"
     CDS             2067..2888
                     /gene="uppP"
                     /locus_tag="VHM69_16435"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX2742034.1"
                     /translation="MLELIEAVILGVVQGLTEFLPVSSSGHLLLGQYFLGLDQDRFGL
                     SFDVALHMGTLVAVVTYFWRDLIRMALAFLRSFGHRDLANDPDQRLAYLVIASTVPAA
                     LIGFLFEGFFENTVRSPWVVVFNFVLVGVLFLVAEAAGNRRRLASKLGFGEAIVIGLA
                     QATALVPGVSRSGATITLGLFLGLRREEAARFSFLMSVPIIAGAGGLKLGEVIAGGMS
                     GEQIAMFLVGFVASAVVGYFTIRFLLNYLTHHSLRVFAYYRFAVAAVVALVLVLF"
     gene            3065..>3356
                     /locus_tag="VHM69_16440"
     CDS             3065..>3356
                     /locus_tag="VHM69_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2742035.1"
                     /translation="MTLSDSSDKKQREVSLADSGETPPGEMEALRQGIVSVDEEIVRL
                     LDRRAWLARRIGEIKHENGLAAYAPARERAVLDRVAALSEGEFPRRGLEAVYR"
BASE COUNT          615 a          970 c         1090 g          681 t
ORIGIN      
        1 gtcgcccgac aagaagtgga gcgacctcac ggccagagag aaagccccct tcgttatcag
       61 gggtgtcgtc cagttcacgc tgctcgtggc ggcgctcgtg gatatctacc gccgtcctgc
      121 ggaggagatc cgcggcagca agtggctatg gagcgcggca gcgttcgtga acttcatggg
      181 cattggtcct atcgtctact tcgctttagg acggaagcga acctaaaggg tggccgcata
      241 agaccaggga ggcagtatga gacagaccgt gctacttcta gtcgccgcgc tggctttggt
      301 gttcttcgtt ggcgcttgcc agacgcaagc ggtcggcgat ctgcagcgcg agacgaacac
      361 gatccaaccc gagaccgccg aggccgtgag cgctcatcta atgatgggcg caggcgagct
      421 aaaggtgagc ggcgccgccg acgccctgat ggagggcgac ttcacctaca acgtggctga
      481 ctggaaaccc aaagtggact acgatgtcct tggcgacacc ggtgagttga gtgtggcaca
      541 gggaagcggc gaggggatcc cattcgccgg cgacgcccgc aacgagtggg acctgcgctt
      601 caacgatcag gtgccgaccg acctcgtagt cgacatgggc gctggagaga gtgatcttga
      661 cctggacagc ctcaacttga cggggctcac cctccacatg ggggcgggca agaccacggt
      721 ggacctcgcg ggcgattaca gccggagctt tgacgccagc atcgagggag gtgtcggcga
      781 ggccacggtg ttgctaccca gcgaggtggg tgtgagagtg accgctgagg gcggcctggg
      841 caagataaac gccgagggct tgaaccggga cggcgaagcc tacgtgaacg acgtgtacgg
      901 agactcggac gttaacctcg aggtggtagt tcgcggtggg gtcggggaga tcaacctgga
      961 ggtagtctga gttctctttg tccggggatg agtagacact ctcctgcacc tgacctgaaa
     1021 gagcatcggt ggcttcgtca catcagagga tgtcgagagg gaaatccccg atcaccttct
     1081 tcgaattggc ccacgggatt tcgtagtgca atagccgtaa tggcggcgaa tgcgccgcgt
     1141 cccgatttgc gcaagcccta aatccatttg tgcagtacaa tgtagcaatg tgtagcacgt
     1201 tcagcctgga gaactcgtga ggatcggcgt ggatttggag ccgcatataa agggaggaga
     1261 ggcgcttggc agttgatgtc tccctcaagg agtatacgaa gctgcaggag caggagtgcc
     1321 gtgcgctttt gcagcttctg gacgaaatgg gcatcgccac cgccgacagg gtctacggac
     1381 ctggcgacgc aatttacaat gagggcgaat acggtgacgc cctgtacgtg ctgacctcgg
     1441 gtgtattgaa gctcttcagg ccatattcgg gcagcaaaga ggcgacgttg cgactcctca
     1501 agccctggga catctttggc catctggcct tcgctggtga gaccaggcag cgcgcctatg
     1561 ccgaggctgt aacggagtgt cgtgtgacga aggtccccaa ggtcttcgtc gaacgcgctg
     1621 ttcgccagga accgcgggtc gccttcaagc tgatgacgct tctggagctg cgtctggtgc
     1681 agtacgagga actggtccag tgcctgctgc cccgcgagac cgaggtgcgc ctggcgaacc
     1741 tgctgcccct actggcgcag aagttcggcg agtacaggaa tggctcggta accatagacc
     1801 tgcgcctcac acaccaggac ctggctgcga tggtcgcctc aacccgggag tcggtcacca
     1861 aggtcctcaa cgaactgcgc aaccggggtg ttatcgaggt tgactccggt cgcatcaccc
     1921 tcaaggacac ccgcgccctc acgatactca gccggccgtg aaatagtatc gcagcacgct
     1981 gaggcttcgc ctcagcgtgc tgaaaagctg acacgctttt gtcatagtac gctggttgcg
     2041 ttggctatgt tgggatggtg ccgggtgtgc tcgagctaat cgaggcggta atcctcgggg
     2101 tggtgcaggg cctcaccgag ttccttccgg tctccagctc ggggcatctc ttgctcgggc
     2161 agtatttcct cgggctcgac caggaccgct tcgggctctc cttcgacgtt gctttgcaca
     2221 tgggcaccct cgtcgctgtc gtcacctact tctggcggga tctcatcagg atggccctcg
     2281 ctttcttacg gtctttcggg catagggacc tcgccaacga ccccgaccag cgactcgctt
     2341 atctcgtgat cgcctcgacg gtgccggctg ccctgatcgg ctttctcttc gagggctttt
     2401 tcgagaacac ggtgcgctcg ccttgggttg tggtctttaa cttcgttttg gtaggcgtgc
     2461 tgtttctcgt cgctgaggct gccggaaacc gaaggcgcct ggcttcaaag ctcgggttcg
     2521 gcgaagcgat agtgataggg ctcgcccagg cgacggcgct cgtgcccggt gtctcgcgct
     2581 cgggggcgac gatcacgctc ggcctcttcc ttggcctcag acgcgaggaa gctgccaggt
     2641 tctcgttcct gatgagcgta ccgatcatcg caggagcggg cggcctgaag ctcggggagg
     2701 tgatcgccgg tggcatgagc ggggagcaga tcgcgatgtt cctggttggc ttcgttgcct
     2761 cggcggtcgt cgggtatttc acgatcaggt tcctgctcaa ttacctcacg catcacagcc
     2821 ttcgcgtctt cgcttactat cgcttcgccg tcgcggccgt cgtcgcactc gtgctcgtcc
     2881 tcttctaaag aacgtgactt tgacatcaga ggccactctc tggtactttg atcggatcat
     2941 gcactacgcg gtccgggcat tgcggcgatt taccggggag gggcgctccc gttcctgacg
     3001 cgcgaccgcg ctcctcctga cccgctccct tgtaaccaga tctcgacagg agaccgcact
     3061 cgtcatgacc ctgtcagact catccgacaa gaagcaacgg gaagttagcc tcgctgactc
     3121 cggagagacg ccgccaggcg agatggaggc gcttcgccag gggatagtct cggtggacga
     3181 ggagatcgtg cgccttctcg accggcgcgc ctggcttgcg cgcaggatag gagagatcaa
     3241 acacgagaac gggctcgccg cgtatgctcc ggccagggag cgggcggtcc tcgaccgcgt
     3301 cgccgccttg agcgagggag agttcccaag gcgcggactg gaggccgtgt accgtg
//
LOCUS       DATAIR010000696         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_211279, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000696 DATAIR010000000
VERSION     DATAIR010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211279"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHM69_16445"
     CDS             <1..327
                     /locus_tag="VHM69_16445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2742036.1"
                     /translation="VVRGWIDLGPDHHTPWGVVHGGVYASAVESAASLGASAAVEEGG
                     QFAVGVNNSTDFLRPMGEGRVEVVAEPIMQGKSQQLWQVIITRAEDGKEVARGQVRLQ
                     NVPLPS"
     gene            complement(334..1479)
                     /locus_tag="VHM69_16450"
     CDS             complement(334..1479)
                     /locus_tag="VHM69_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2742037.1"
                     /translation="MRDGREDVKVDIAVVGAGTMGAGIAESVALAGMSVTMLDVGEEA
                     LERGRHTIERDLERRVKKERLSEEESRDVLDRISTTTSVGDLSGVSLVVEAVVEDMEV
                     KRKVFADLEGAVGDEAVLATNTSSLSVSGIATATENPERVVGMHFFNPVPAMRLVEVV
                     AGPSTEESALRRAEEVAGWLGKTPVRVSDTPGFIVNRVAQPFYLEALRIVESGGAPAQ
                     IDAEIRGAGFRMGPLELADLIGLDVNLAVSESLFERYYCHPRFRPSFLQRSMVEAGTL
                     GRKSGRGFYDYRDERRGEISAPSEEEVARRIISCIVNEAFLAHSEGVATTEDIDRAMK
                     LGANYPKGPFEWADEIGAASILNTLDSLRATHGDAYVAAPALREIVR"
     gene            complement(1616..1792)
                     /locus_tag="VHM69_16455"
     CDS             complement(1616..1792)
                     /locus_tag="VHM69_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX2742038.1"
                     /translation="MIGSDLSDAQGCFESGARMSGTDSDGADVSGADLTNAKVTGEQL
                     REAEALDDATMPNG"
     gene            complement(1988..2500)
                     /locus_tag="VHM69_16460"
     CDS             complement(1988..2500)
                     /locus_tag="VHM69_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742039.1"
                     /translation="MEEKGKQEAAGEKQSRQPWTLREFGGKTLWDWLQLLIVPIMLSL
                     ITVAFTWQQNTRQQRLEDQRARQAQRIENQRAQAEQEIQEQRAQQATLQAYSDQMGTL
                     LLDRGLRNADEDSDVRRLARARTLVVLDALDSDRQNRVFRLLKETELIQARPPDVRQS
                     SASSTPACTT"
BASE COUNT          459 a          851 c          788 g          583 t
ORIGIN      
        1 gtggtacggg gatggattga tctcggcccg gaccatcata cgccgtgggg ggtggtgcat
       61 gggggcgttt acgcctcggc ggtcgagagc gccgccagcc tgggtgcttc agccgccgtg
      121 gaggagggag gacagttcgc cgtcggcgtc aacaactcca cagactttct gaggccgatg
      181 ggcgaagggc gggtggaggt agtagccgag ccgatcatgc agggcaagag ccagcagctc
      241 tggcaggtca tcatcacccg cgctgaagat ggcaaagagg tagcccgcgg tcaggtgcga
      301 ctgcaaaacg taccattacc gtcctgacag ttcctaacgt actatctccc tgagcgccgg
      361 ggcggccacg taagcgtcgc cgtgggttgc cctcagggag tcgagtgtat tgaggatgct
      421 agccgccccg atctcgtccg cccattcgaa cggacctttg gggtagttcg cgccgagctt
      481 catggcccgg tctatatctt cggtcgtcgc cacaccctcc gaatgggcca ggaacgcttc
      541 gttcactatg caggagatga tgcgccgcgc cacttcttcc tctgagggcg ctgaaatctc
      601 cccccgcctt tcgtcacgat agtcatagaa cccccgtccg ctcttcctgc caagcgttcc
      661 ggcctcgacc atcgaacgtt ggaggaaaga aggacggaac ctcggatggc agtagtatcg
      721 ctcgaagagg gattctgaga ctgcgaggtt gacatcaagg ccgataaggt cggcgagttc
      781 cagcggtccc atcctgaacc ccgccccgcg gatctccgcg tcgatctgcg cgggcgcgcc
      841 ccctgactcc acgatcctca gggcttcgag gtagaacggt tgggccacgc ggttcacgat
      901 gaatcccggc gtgtccgaga cacgcaccgg cgtcttgccc agccatccgg cgacttcttc
      961 cgcccgtctc agggcagact cttccgtgga aggcccggcg acgacctcga ccaggcgcat
     1021 tgcgggtacc gggttgaaga agtgcatccc caccacccgt tcggggttct cggtggccgt
     1081 cgcgatgccg gacaccgaga gcgaggaggt gttggtcgcg agcacggcct catctccaac
     1141 cgctccttcc aggtccgcga agaccttcct ctttacctcc atgtcctcga ccaccgcttc
     1201 gacgacgagc gaaaccccgg agagatctcc caccgaagtg gtagtcgaga tgcggtcgag
     1261 cacgtccctg ctctcctcct ccgagaggcg ttctttcttc acccggcgtt cgagatccct
     1321 ttcgatggta tgcctgccgc gctccagcgc ctcctcgccc acgtcgagca tggtgacaga
     1381 catccccgcg agcgccacgc tctccgcgat gccagcaccc atcgtgccag cccccacaac
     1441 agcgatgtcc acctttacgt cctcccgtcc gtccctcacg tgtcccagtc cacgcggttg
     1501 attgtacgga cggtcgagag tactgcaaag ttcgccttga tggatgttac gaggggccgc
     1561 tgttctcccc accctccctg cggccttatt tgtctctgag ccagtcttcg tacttctagc
     1621 cgttgggcat ggtggcgtca tccagggcct cggcttctcg tagttgctcc ccggtgacct
     1681 tggcgttggt aaggtcggcc ccactgacgt cggcaccatc tgaatcagtg ccactcatgc
     1741 gggcgccgct ctcgaagcat ccctgggcgt cactcaaatc agagccgatc aagctggctc
     1801 cgctggggtc agcatcacgg agataggctc cgctcagctt ctctcaagtc agcaccgctg
     1861 aggttggccc cctccagata ggccccgctc atgtcggtac cttgcaggtt ggctttggtg
     1921 agatctgctc cggagaggtg agcttgagtc aggtcactac tttcgaggag ctgtttccct
     1981 ttgagtttta agttgtacaa gcgggcgtac ttgaggctga tgattggcgg acgtccggcg
     2041 gccgggcttg tatgagttca gtctctttca aaagcctaaa taccctgttc tggcgatcag
     2101 agtccagcgc gtccagcact accagcgttc tggctcgcgc tagcctcctt acgtcgctgt
     2161 cctcgtcggc gttacgcaga ccgcgatcta gcaaaagcgt tcccatctgg tcgctgtaag
     2221 cctgcaacgt agcttgctgg gcacgctgct cctgtatctc ttgctcggct tgtgcgcgtt
     2281 ggttctcgat cctctgtgcc tgtcgggcgc gttggtcctc gagccgttgc tgccgagtgt
     2341 tctgttgcca ggtgaacgcg accgtgataa gagacagcat gatcgggaca atgaggagtt
     2401 gcagccagtc ccagagggtc ttcccgccga actctctaag cgtccacggc tggcggcttt
     2461 gcttctctcc cgcagcttcc tgcttgccct tttcctccac tgtgagcgat acgataacag
     2521 agctggtgaa tccacgatga atcgaggccc tgcatgccta tccagcacca caatatgtac
     2581 ctctcggcat tgccgccgcc gatcatttat tgttacggta ggtggaaata agattctctg
     2641 ttggcaggcg ttcgtgatca gggacgctgc ggtcggcgag g
//
LOCUS       DATAIR010000697         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_349124, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000697 DATAIR010000000
VERSION     DATAIR010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349124"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..603)
                     /locus_tag="VHM69_16465"
     CDS             complement(25..603)
                     /locus_tag="VHM69_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742040.1"
                     /translation="METRLSAVVPTGYPSGTIATVAAGLVEFSATLSWKRAEDVPSSP
                     TLPALPQTVRCSVVENDDPGRREISGEILGLYEFDLGSALETVLRSLTITAALSDALL
                     ETRAARVTPPSSASAATLQALALDLWNAGFHVRFGPSWTPVPEGAIGLGVSGAEEELR
                     GFLEAHPSWEMSWEFQDAVLDRHGDPGYSLKA"
     gene            complement(630..2276)
                     /locus_tag="VHM69_16470"
     CDS             complement(630..2276)
                     /locus_tag="VHM69_16470"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2742041.1"
                     /translation="MSETKYIFVTGGVVSSIGKGTSAAALGMLLKSRGYRVVLQKFDP
                     YINVDPGTMNPYQHGEVFVTEDGAETDLDLGHYERFLDENLGRLSNVTTGSVYWEVIT
                     RERRGDYLGATVQVIPHITNEIKNRIGRLGQDKDVVITEIGGTVGDIESLPFLEAIRQ
                     FRNDVGRKNVLYVHVSYVPYIEAAGELKTKPTQHSAQRLREIGISPDVLICRADRPIS
                     DDIRRKIALFADTELDSVIAAEDAPNLYAIPLNLHREGLDDLVLDKLGLAAPPVDLGE
                     WRNLVSRMLEATRTVRVAIIGKYIKLQDAYLSVVEALAHAGGAHDLRVELDWVDSEQL
                     VDRASTEKRLAGVDGVLVLPGFGHRGAEGKVEAARYARETGTPYLGLCLGMQIEVIEF
                     ARNVAGLEMSNSTEFDPDTPHPVIDIMPDQVGVEMGGTMRLGRWPCQIVPGTLAERVY
                     GADLVYERHRHRYEVNNAYRDALSDAGMVFSGTSPDGRLVEIAEIKDHPFYIGSQFHP
                     EFKSRPLRPHPLFYGFMGACGAALEEAADIEAVPGERAAR"
     gene            complement(2477..>3218)
                     /locus_tag="VHM69_16475"
     CDS             complement(2477..>3218)
                     /locus_tag="VHM69_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742042.1"
                     /translation="DRLAAMRALERKYGDVEGYLEESRARLARLENLDEETAELEERL
                     AEGERRLGDLAATLTAGREAAGERLSVKVRENLGDLNLGKTTFRAELMRAEPGPSGRE
                     RVEFVIQPNPGEPELPVRRYASGGELSRIMLAIRLAQERVEPGATYVFDEVDAGIGGE
                     TATAVGAKLRELGERNQVLTITHLPQIASEAGTHVVVEKEESGGRTITRIERIKGEER
                     RRELARMLSGRIDEASLAHASELLVGGR"
BASE COUNT          569 a         1076 c          955 g          618 t
ORIGIN      
        1 agacttcgta ggaatattgc ggtctcatgc tttcaaagag taccctggat cgccgtgacg
       61 atccagcacc gcgtcctgaa attcccagga catttcccag gaaggatgcg cctccaggaa
      121 gccccgcagc tcctcctccg ccccactgac cccgaggccg atcgcacctt cgggtaccgg
      181 cgtccacgat gggccgaacc tgacgtgaaa accggcgttc cacaggtcga gagcaagggc
      241 ctgcaacgtc gccgcagaag ccgatgatgg tggcgtcacg cgggcggcgc gggtctcgag
      301 gagggcgtcg gagagcgccg ccgtgatcgt cagggaccgc agtacggttt cgagggccga
      361 accgaggtcg aactcgtaga ggccgaggat ctcaccgctg atctcacgcc ttccagggtc
      421 gtcattctcg acgacggaac accgcacggt ctggggcaac gcggggagcg tgggcgaaga
      481 aggcacgtcc tcggcccgct tccaggagag cgtcgccgaa aactcgacca ggcccgccgc
      541 gacggtggct atggtaccgg aagggtagcc cgtcggtacg acggcggaga gtcttgtttc
      601 caccagaccc cgcactacgc cggcttccct cagcgggccg cccgttctcc aggaacggct
      661 tctatgtcag cggcctcttc cagcgccgcg ccgcaggccc ccatgaaacc gtagaacagt
      721 gggtgtggcc gcaaggggcg gctcttgaac tctgggtgga actggctccc gatgtagaac
      781 gggtggtctt tgatctccgc gatctcgacc agcctgccgt cgggtgaggt ccctgagaag
      841 accatccccg catcagagag cgcgtccctg taggcgttgt tcacctcgta gcggtggcgg
      901 tgacgttcgt agaccaggtc cgcaccgtag accctctccg cgagtgtgcc tggcacgatc
      961 tggcagggcc agcgtccgag gcgcatcgtc ccgcccatct cgacgccgac ctggtccggc
     1021 atgatgtcta ttactggatg gggggtgtcc gggtcgaact cggtggagtt cgacatctcc
     1081 aggcccgcga cgttgcgtgc gaactcgatg acctctatct gcatcccgag gcagagtccc
     1141 aggtagggcg ttcccgtctc ccgggcgtag cgtgccgcct ctaccttgcc ctcagcgccg
     1201 cgatggccga agcccggcaa aacgagcacc ccgtccaccc cggcgagccg cttctcggtc
     1261 gatgcccggt ccacgagctg ctcggagtcg acccagtcga gctcgaccct caggtcatgc
     1321 gccccaccgg cgtgcgcgag ggcttccact acagacaaat acgcgtcctg aagcttaatg
     1381 tacttgccga tgatggccac cctgacggta cgcgtggctt ccaacatgcg cgaaacgagg
     1441 ttacgccact ccccaaggtc cacggggggc gcggcaagac ccaatttatc cagcacaaga
     1501 tcgtcgagac cttcgcgatg aaggttaagt gggatggcgt agaggttagg cgcgtcctca
     1561 gccgcgatga ccgagtcgag ctccgtgtcg gcgaagagcg cgatctttct cctgatatca
     1621 tcgctgatgg gcctgtcggc cctgcagatg agcacgtccg ggctaatgcc gatctcacgc
     1681 aggcgttgcg ccgagtgctg ggtcggcttg gtcttcaact cgcccgcggc ctcgatatag
     1741 ggtacgtagg agacgtggac gtagagtacg ttcttgcgtc cgacatcgtt tctgaactgc
     1801 cttatcgcct ccaggaaagg cagactctcg atgtcgccga cggtgccgcc aatctcggtt
     1861 atgacaacgt ccttgtcctg cccgagacgc cctattcggt tcttgatctc gtttgtgatg
     1921 tgcggaatga cctgcacggt cgctccgagg taatcccccc tcctctccct ggttatgacc
     1981 tcccaataga cgctccccgt cgtcacgttc gagagcctgc ccaggttctc gtccaggaac
     2041 cgctcgtaat ggccgaggtc gagatccgtc tcggccccat cctcggtgac gaacacctca
     2101 ccgtgttgat aagggttcat cgtccctggg tccacgttga tgtacgggtc gaacttctgg
     2161 agcacgaccc tgtacccacg gctcttgagc aacataccca gagccgcggc gctggtcccc
     2221 ttgccgatgg acgaaacgac gccgcccgtc acaaagatat acttcgtctc gctcacctac
     2281 accacactcc caacagacat aaacaacgta acgcccctac ctcctgtatg gatcgggatc
     2341 agggaagaga atccgacgga gaagcgcggg ctgatcggct cccgcaacct tctacgccag
     2401 cgcacctgca tggatctcta tcatacgtct tttggcgcaa gaattaaaca gcgtgggatg
     2461 ataagcgagc ggacggttac cttccaccta caaggagctc tgaggcgtgg gcgagtgagg
     2521 cctcgtcgat gcgccctgag agcatacggg caagctccct gcggcgctcc tcgcccttga
     2581 tgcgctctat gcgagtgatc gtacgtccac ccgactcctc tttctcgact acgacgtgcg
     2641 tgcccgcctc ggaggctatc tgcgggaggt gtgtgatcgt cagtacctga ttgcgctcgc
     2701 cgagttccct cagcttagcc ccgacggcag tcgccgtctc gcctcctatc ccggcgtcga
     2761 cctcgtcgaa gacgtacgtc gcgccaggct caacgcgctc ctgcgcgagc cttatggcga
     2821 gcatgatcct ggagagctcg ccgcccgagg cgtagcgtct tacaggcaac tcaggctcgc
     2881 cggggtttgg ctggatcacg aactccaccc tctctctacc ggagggtcca ggctcggccc
     2941 gcatcagttc agcccggaag gtcgtcttcc cgaggttgag gtccccgagg ttctcccgca
     3001 ccttcaccga caacctctcg cctgccgcct cccttcccgc ggtcagcgtg gccgcaaggt
     3061 caccgaggcg ccgctcacct tcagcgagcc tctcctcgag ctccgcggtc tcctcgtcca
     3121 ggttctcgag ccttgcgagg cgcgcgcggg attcttcgag gtaaccctcg acgtctccat
     3181 actttcgttc cagggcacgc atggcggcga gcctgtcc
//
LOCUS       DATAIR010000698         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_349147, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000698 DATAIR010000000
VERSION     DATAIR010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349147"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..949)
                     /locus_tag="VHM69_16480"
     CDS             complement(32..949)
                     /locus_tag="VHM69_16480"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2742043.1"
                     /translation="MASLAAALLVGVMLVCAQVWGAPLVQALGLEQNAPLGKLVNLNA
                     THAALEEPRSDGLAAGDPINVLVLGVDAKPEDEHGGSARRSDTIMLVQVSPDSGMVKL
                     LSMPRDLMVEVEPGVEGKINSAYAYGGISQTISALENYADIPIDHYAIVDFEGFEAVV
                     DDIGGVRLDVEEGDLPPDVRLKKEGVQHLNGRRALLYARYRGTAGGDLDRIERQQELV
                     AALRSKALHWNGVRRLPQVAATIEKHVETDMGVRDAVALGSVLLKHGRGARMTATQLK
                     GTPETLPNGVEVLKPDHQVNQALLREFRY"
     gene            1234..1986
                     /locus_tag="VHM69_16485"
     CDS             1234..1986
                     /locus_tag="VHM69_16485"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HEX2742044.1"
                     /translation="MTTFWILLLFAIIFLVAGGVMLYFRRSTQQQSALMSQTETSSAS
                     EVSGLSPGTTVEVKGTLRCDEPLTSEMAGEACAYYQATVTREYLEEDHDDNDSGSDRR
                     SEVLSQSERFAPFMVEDESGRVPVNAEGAEMDVRQVVDRFERDADSGFSLGGIPIRFG
                     DSERTIGYRYTESLLPVDKPVYVLGTVRENGEIGAPPAEDKHQRFVVSYRSEEALGQS
                     LGRNVFWLGIGAIGAFVLGVVLLAIGLLLLVS"
     gene            complement(2005..2077)
                     /locus_tag="VHM69_16490"
     tRNA            complement(2005..2077)
                     /locus_tag="VHM69_16490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2042..2044),aa:His,seq:gtg)
     gene            complement(2406..2696)
                     /locus_tag="VHM69_16495"
     CDS             complement(2406..2696)
                     /locus_tag="VHM69_16495"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2742045.1"
                     /translation="MYARSGTLYPRPGEFDYVLRLLRDTVALAAQREPGFSGMLIMND
                     RQASKVVGITLWESKADMLASAEGEYLQEQVSRIITHLKKPPEFEDYELDLM"
     gene            complement(2920..2993)
                     /locus_tag="VHM69_16500"
     tRNA            complement(2920..2993)
                     /locus_tag="VHM69_16500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2957..2959),aa:Arg,seq:tct)
     gene            complement(3021..3091)
                     /locus_tag="VHM69_16505"
     tRNA            complement(3021..3091)
                     /locus_tag="VHM69_16505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3057..3059),aa:Gly,seq:tcc)
     gene            complement(3105..3178)
                     /locus_tag="VHM69_16510"
     tRNA            complement(3105..3178)
                     /locus_tag="VHM69_16510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3142..3144),aa:Pro,seq:tgg)
BASE COUNT          697 a         1048 c          947 g          652 t
ORIGIN      
        1 cacaaataac acaacgcggg tacgacaaag tctagtagcg gaactctctc aagagcgcct
       61 ggttaacctg atggtccggc ttcagcacct ccacgccgtt cggcagggtc tctggagtac
      121 ccttgagctg cgttgcggtc attctcgcgc cgcgtccgtg cttgagcagg acactcccca
      181 gagccacggc gtcacggaca cccatgtccg tctcgacatg cttctcgatg gtggctgcga
      241 cctgcggaag cctccgcacg ccgttccaat gtagagcctt gctcctcagc gccgcgacga
      301 gttcctgctg gcgctctatt cggtcgagat ccccccccgc ggtgccgcga taccgcgcgt
      361 agagcagcgc tcgacgcccg ttcaggtgct gcacgccctc tttcttcaag cgcacgtcgg
      421 gaggaagatc tccttcctcg acatctagcc taacgccccc gatatcatcc accacggctt
      481 cgaaaccctc gaagtctaca atagcgtagt ggtctatcgg aatgtcggcg tagttttcca
      541 gggcggagat cgtctgcgag ataccgccgt aagcatatgc agagttgatc ttgccctcga
      601 ctccgggctc cacctcgacc ataaggtccc gcggcatcga cagcagcttg accatgccgc
      661 tatccggtga gacctgcacc agcataatag tatccgaacg tctcgccgaa ccgccgtgtt
      721 catcctccgg cttggcgtcc acgcccaata ccaggacgtt gattggatcg cccgccgcca
      781 acccatcact ccgcggctct tctagcgccg cgtgcgtcgc attcaaattg acgagcttcc
      841 ccaaaggtgc attctgctca agcccgagag cctgaaccag cggcgcgccc cacacctggg
      901 cacagacgag catcacaccc accaacagcg ccgccgcaag cgatgccacc agcaaccccc
      961 gccgccgaca cgagcccgtg ctgcgcgtat attcctggaa aaccctcacc cacgggagtc
     1021 taatgccccc accacactat cgcaacgcat cacatatccc acacgtgtaa gatggtttcg
     1081 aacccacgat cttgagaggc acgatactat cgccagtgca agacagatgc agcatcttga
     1141 acgccacgac acggccactg cgattgtctc ctactcaccg cgccgtccga tgcgcgaatc
     1201 attccaggaa gcgaccagta aggggagctg aatatgacga ccttttggat actgttgctg
     1261 ttcgcgatta tctttctggt agctggcggc gtcatgctgt atttccggcg gagcacgcag
     1321 cagcagtcgg cgctaatgag ccagaccgag acgtcgagcg cctccgaagt atccgggctc
     1381 tcaccgggaa ctacggtcga agtcaagggt accctgcgat gcgacgagcc gctgacgagt
     1441 gagatggccg gcgaggcttg cgcttactat caagccaccg tgacacgaga atacctggaa
     1501 gaagaccacg acgacaacga ctccggctcg gatcggcgct cggaggttct ctcgcaatcc
     1561 gagcggttcg cgcccttcat ggtcgaagac gagtcgggca gagtgccggt gaacgccgaa
     1621 ggcgcagaaa tggacgttag gcaggtggtg gaccgcttcg aacgcgacgc ggactccgga
     1681 ttctccctcg gcggcatacc gatcaggttc ggcgacagcg aacgcaccat cggctatcgg
     1741 tatacggaga gccttttacc ggtggacaaa ccagtctacg tgctgggaac ggttcgggag
     1801 aacggtgaga tcggggctcc gccagcggaa gacaaacacc agcggttcgt cgtcagttac
     1861 cgctcggagg aagccctggg acagagcctg ggaaggaatg tattctggct gggaatagga
     1921 gctatcggcg cgttcgtcct gggagtggtc ctgttggcta tcggactctt attgctggtc
     1981 agctaggaac gcggacgccg taagggtgac cgacggggct cgaacccgcg accttcggga
     2041 ccacaacccg atgctctgcc aactgagcta cgatcaccac gcgcagaggg attttatcag
     2101 gaaaggcccc ggaccaaaac gtagtgttgc ttatgcacca ccacagatga accgggtctc
     2161 gcggtcttct atgcgcgcgc tacggtcccc tagcactttg tcaaccgcga gtttgaatgg
     2221 aggcttccag ggcgcggtga tgtggttctc acactcatct tttggagggg accctggaat
     2281 cgaagggatc tagcagatcg agttctatcc cgatgggcgg ccctgcaccg aacgtcgcgt
     2341 ggcccttccc gggctgagac gaggattctg aaccagaagc gaggaccagg caaaatcctc
     2401 tgcttctaca tcaagtcgag ttcgtagtct tcgaactcgg gcggcttttt caagtgggtt
     2461 atgatccggc tcacctgttc ttgcaggtac tcgccctccg cgcttgcgag catgtcggcc
     2521 ttgctctccc acagggtaat cccgacaacc ttactcgcct ggcggtcgtt catgatcaac
     2581 atcccactaa agcccggttc cctctgggcg gccagggcta ccgtgtcgcg cagtagtcgc
     2641 agcacgtaat cgaactcgcc ggggcgcgga tacagagttc ccgaacgcgc gtacatggca
     2701 tccccttttg ctggttaggg caacaattgt caagtgtacg ctgtactcca caagaacggt
     2761 agatatttgc ctaaatgagg tgttaagaag cctccaatcc ccaccgctgc ttctccaaac
     2821 tctacttatc gttcagattg gccgctcgag ccgacaataa tggattagtc cgactcttga
     2881 tacaaaaacc atgtcacccg gttccgggtc ttccacatgc gcgcccggca ggactcgaac
     2941 ctgcaacctg cggattagaa gtccgctgct ctatccagtt gagctacggg cgcttgttgg
     3001 agggccctag cggacccgag agcgggtgag gggaatcgaa cccccatcat cagcttggaa
     3061 ggctggggct ctaccattga gctacacccg cgtgatcccg gccgtcgggg cgagaggatt
     3121 cgaacctccg accccctgct cccaaagcag gtgcgctacc aggctgcgcc acgccccgag
     3181 atgccaggag caagtataac aaacgggatg atcctttaaa ctgcggtggt cggggctacg
     3241 gctcgtatct cctcgtccag gaagcggctg cccagggcgg cgaagtcatc ggggtcggcg
     3301 ctacagatgt aggtagcaga cccgagatcc tccgcgtcgt gggg
//
LOCUS       DATAIR010000699         6309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_257363, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000699 DATAIR010000000
VERSION     DATAIR010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257363"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VHM69_16515"
     CDS             complement(<1..184)
                     /locus_tag="VHM69_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742046.1"
                     /translation="MSAGTMGLETARDGLRRPLLIFASSATIFSVLHHTDHVIRGNHS
                     GWPFQAEVTPFTFSLLI"
     gene            complement(181..681)
                     /locus_tag="VHM69_16520"
     CDS             complement(181..681)
                     /locus_tag="VHM69_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742047.1"
                     /translation="MKKEATMCGRSSVGYGIGHGLRRHSGGRDFLGRDVEKMSPGARV
                     MAALSVLVPVGLSGVFLVVFVPQLWWIFTTYFWVAFPAFGLLSSSVAGLGEGRVSEQE
                     RERELLEAIRERGEISAAAAAARTSLTVAEADRRLRELAENGHLEVRARGGAILYALW
                     GSEELR"
     gene            767..1357
                     /locus_tag="VHM69_16525"
     CDS             767..1357
                     /locus_tag="VHM69_16525"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2742048.1"
                     /translation="MTTLGYAILGLLSRESLSGYDLTQRMKGPVGYFWSARHSQIYPE
                     LARLEEGGYVTHKVVEQKERPDKKVYEITDEGLEELKGWVTRPPVPRPTRDELTLKTY
                     SVWLADPEEATRIFREEEERHEEQLAHYEEIRAWMEREWRDGLTRTDSPEFASYATLR
                     RGISYEREYAGWCRWMADSIGGGKSPENHKEDKNRG"
     gene            1403..2584
                     /locus_tag="VHM69_16530"
     CDS             1403..2584
                     /locus_tag="VHM69_16530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742049.1"
                     /translation="MMELRRYREGDIGALARLGVAAFGASVSDWEEYFDPVRNARLDP
                     EQVHVIEEDGEARASATVLPLESFVDGEPRPMGGIAAVMAHPAYRRRGHAGELMRAVL
                     RDMRERDIHLSLLAPFAHSFYRMFGYELATEGIEYRFRPTDLATSPEQSHLRAYREED
                     LPQMMALLEAEAREHPLCVRRSEGHWKATLAEKDKEVAVYERDGGVEGYILYKMSGWR
                     EDRDPRRILSIREAVGATQRAWEAILSFLAGLDPQVFEIHLYTSRGEPIHPYLRSSHV
                     NAKVAPDQMLRLVDVEGALGYLNRKIDSPLVLDVSDDVIPENDGEYTFGESDVVRGAE
                     AEARVSLDVRHLAQLYAGYLPVRGLARHGLIQAESPEALELLETLFPVGDPTLFGPDH
                     F"
     gene            2751..3752
                     /locus_tag="VHM69_16535"
     CDS             2751..3752
                     /locus_tag="VHM69_16535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2742050.1"
                     /translation="MKPTGTIGYAAMFEQFHPTELLAWCEVAEESGFGSVMASDHFHP
                     WTPDQGQSAFVWSWLGALGATTNLRFGTGVTPPGFRYHPAIIAQAAATLEAMYPGRFW
                     LGLGAGEALNEHIVGDYWPEAPTRLRILMEAIEVIRRLFSGKVVKYSGEHIKLESARL
                     YTMPDTPPPIYVATAGPIQSRRTGEFCDGIITVGAPDEKIRGLMEKFEEGARRADKDP
                     SNMPRMIQLHVSWAEDQEEAEQQAVREWPNGGMPFPKADIRNPEDFAAMAKQVTVENF
                     KNRVLMSADLEEHLEHIQHYVDLGFDEVYVHNVGRNQEQFIRAYGERIIPKLRWPER"
     gene            3782..4015
                     /locus_tag="VHM69_16540"
     CDS             3782..4015
                     /locus_tag="VHM69_16540"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2742051.1"
                     /translation="MDWRDHINSDPEILSGKPVVKGTRLSVEFLLGLFAVGWTKEQVL
                     ENYPTLDSEAIRAVFAFAAEHVREESVSLGTRG"
     gene            4031..4384
                     /locus_tag="VHM69_16545"
     CDS             4031..4384
                     /locus_tag="VHM69_16545"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX2742052.1"
                     /translation="MRFLADENFPLRGVGFLTNAGHEVAAIILESPGLPDEQVMLRAA
                     REDRVLLTVDRDHGRLLYRTGVVYFRFDPAYPEEPVEHLLSILDQPETSVLGMLTVME
                     RDRVRQRPLPGADRT"
     gene            4539..5633
                     /locus_tag="VHM69_16550"
     CDS             4539..5633
                     /locus_tag="VHM69_16550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2742053.1"
                     /translation="MKVAVVGAGIIGSSVAYRLSEGGAKVVLIDAAEPGSGTTSASFA
                     WVNANNKLPREYFELNLAGMREHEQLRDEIGGGWLHPTGNLIQTTDEGQEELEKRVGR
                     LRSWSYAAEMLTASTVNEKLEPEAVFPNPETRIAYFPEESWVDAPALTRTLVQAARRN
                     GALTLVGNAVHGIEVGGEGVTLRLEKGDAVHADAVVNATGAGAASVAEMVGRKLPLDV
                     FRGLLVRVAVPGDPLGRLIHTPRVNLRPDGPGYVLLNHDSVDAKLTDDFSGTEDPLCA
                     ELLERARRVFPALAEAEVVEARFGLRPVPADGHSCVGELSEIPGYYEAVTHSGVTLGP
                     LVGRLLAREILTSQVDPLIAPFRPDRFPST"
     gene            5908..>6309
                     /locus_tag="VHM69_16555"
     CDS             5908..>6309
                     /locus_tag="VHM69_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2742054.1"
                     /translation="MARTLFSGGTIVTAEGSYRADVLVEDEKVVAVGTNLDADGAETV
                     DASGKLVMPGFIDAHTHMDMPFGGTVTADDWATGTEAAAAGGTTMIIDFALQEAGGTL
                     AGAFDTWTEKARDKAVIDYSFHVAITDLRDDI"
BASE COUNT         1292 a         1934 c         1996 g         1087 t
ORIGIN      
        1 agatcagcag gctgaacgtg aacggcgtga cctcggcctg aaagggccat cccgagtgat
       61 tacccctgat gacgtggtcc gtatgatgca gcacgctgaa tatcgtcgcc gacgaagcga
      121 agatgagcaa gggtcgccgc aaaccgtccc tggcggtctc caaacccatc gtgccagcgc
      181 tcaccttaat tcctcgctcc cccacaacgc atacaggatg gcgcctccgc gagccctgac
      241 ctcgaggtgg ccgttctcgg ccagctccct gagccgcctg tcggcctcgg ccaccgtcag
      301 cgaggtcctc gccgccgcag ccgccgccga gatctcaccg cgctccctga tcgcctcgag
      361 caactcccgc tcccgctcct gctctgaaac ccgcccctca cccaaccccg ctacgctact
      421 gctcaacagc ccgaacgcag ggaacgcaac ccagaagtac gtcgtgaaga tccaccacaa
      481 ctggggcacg aacacgacca gaaagacccc cgagagcccg acaggtacca gaaccgagag
      541 cgcggccata accctcgccc caggactcat cttctccacg tcacgcccca gaaagtcacg
      601 cccgccacta tgccgcctca gtccatgacc tatcccatac cccacagaag acctgccgca
      661 catcgtagcc tccttcttca tgtcctttat gtcactcctg acatatggta ctctacaatc
      721 agattgctga tatgtcaaga gtgacataag agttttttgg aggatcatga cgacgcttgg
      781 gtatgcgatc ctgggtctac tatcgaggga gtcgctcagc gggtacgatc tgacgcagcg
      841 catgaaggga cctgtcgggt atttctggag tgcgaggcac agccagatct acccagagtt
      901 ggcgaggcta gaagagggcg gctacgtgac ccacaaagtg gtcgagcaga aggagaggcc
      961 ggacaagaag gtctacgaga ttaccgatga gggtctggag gagctgaagg gttgggtaac
     1021 ccggccgccc gtgccgaggc cgacgcgaga cgagttgacg ttgaagacgt actcggtctg
     1081 gctcgctgac ccagaggaag cgacgcggat cttccgtgag gaggaggagc gtcacgagga
     1141 gcaactcgcg cactacgagg agatccgggc ctggatggag cgcgagtggc gcgacggcct
     1201 gacccgtacg gacagccccg agttcgcaag ctacgcgacg ctcaggcgcg gcatcagcta
     1261 cgagagggaa tacgccgggt ggtgccggtg gatggccgac tccatcggag gcgggaaaag
     1321 tccggagaat cacaaagagg acaagaaccg cgggtgatac gctcttccga tagaatccag
     1381 gcgcagtcta gagagccgga ggatgatgga gcttcgcagg tatcgtgaag gggacatagg
     1441 cgcgctggcg cgtctcggag tggcggcctt cggggccagc gtctcggatt gggaggagta
     1501 cttcgatccc gtgcggaacg cgcgcctgga ccctgaacag gtccacgtca tcgaggagga
     1561 tggcgaggcg cgggcgagcg ccacggtgct gccgctcgag tctttcgtgg atggagagcc
     1621 gcgtccgatg ggcggcatcg ccgcggttat ggcccatcct gcatacagac ggcggggcca
     1681 cgctggggag ttgatgcgcg ccgtgctgcg cgacatgcgc gagagggaca tccatctctc
     1741 gctactggcg cccttcgccc actccttcta caggatgttc ggctacgagc tcgccacaga
     1801 gggcatcgaa tacaggttca gacctaccga tctcgccacc agccccgaac agagccatct
     1861 cagggcctac cgtgaggagg acctgccaca gatgatggct ctcctcgaag cggaggcgag
     1921 ggaacatccc ctgtgcgtgc gccggagcga gggacactgg aaggccaccc tggcggagaa
     1981 agacaaagaa gtcgcagtat acgaacggga cggaggagtg gagggctaca tcctctacaa
     2041 gatgtcgggg tggcgcgaag accgcgaccc gcgccgtatc ctctcgataa gggaggccgt
     2101 cggcgccaca cagcgggcgt gggaggccat cctctccttt cttgcgggcc tggatcccca
     2161 ggtcttcgag atacacttgt acacttcgcg cggggagccg atacacccct atctcagaag
     2221 ctcccacgtt aacgcgaagg tcgcgcccga ccagatgttg cgcctcgtag acgtagaagg
     2281 agctttagga tacctgaatc ggaagatcga ctcaccgctc gtcctcgacg tctcggacga
     2341 cgtcatacct gagaacgacg gagagtacac cttcggagaa agtgatgtcg tccgtggagc
     2401 ggaagccgag gcgcgggtgt cactcgacgt gcgccaccta gcccagctat acgcagggta
     2461 tctcccggtc agaggtctcg cccgtcacgg gctcatccag gcggagtctc ccgaagccct
     2521 tgagttgctg gagaccctct tccctgtggg agacccgact ctgttcgggc cggatcattt
     2581 ttgagttgag taggctacag gcatcagcca ctctgtgagt tggcgacacc ttcgagggtt
     2641 ggcggaggtc ttctccatag tcgctgtaaa ctatcgggtg gcgggcagga gaagctccgt
     2701 ccagcgaaaa cctgaaacct gatacctcat ccgacgaagg aggcatcaca atgaaaccca
     2761 cgggcaccat aggctatgcg gcgatgttcg agcagttcca tccgacggaa cttcttgcgt
     2821 ggtgcgaagt ggccgaggag tcggggttcg gctctgtcat ggcctccgac catttccatc
     2881 cttggacccc ggatcagggc cagagcgcct tcgtctggtc ctggttgggc gctctgggtg
     2941 cgacgacgaa cctccgtttc ggaaccggcg tgaccccgcc gggcttccgc taccatccgg
     3001 ccatcatcgc gcaggccgcg gcgacgctcg aagcgatgta ccctggacgc ttctggctag
     3061 gactgggtgc cggagaggcc ctcaacgagc acatcgttgg cgactactgg cccgaggcgc
     3121 cgacccgcct ccgcatcctc atggaggcga tcgaggtcat ccgcaggctc ttctcgggga
     3181 aggtcgtcaa gtacagcggc gagcacataa agctcgagag cgcgaggctc tacacgatgc
     3241 cggatactcc gcccccgatc tacgtcgcaa ccgcggggcc catacagagc cgcaggacgg
     3301 gcgaattctg cgacggcatc atcaccgtgg gcgccccgga cgagaagata cggggcctga
     3361 tggagaagtt cgaggagggg gcgcgcaggg ctgacaagga cccctccaac atgccgcgca
     3421 tgatacagct ccacgtctcg tgggcagagg accaggaaga ggccgagcag caggccgtac
     3481 gcgagtggcc caacggcggg atgcccttcc ccaaggcgga tataaggaac cccgaggact
     3541 tcgccgcgat ggcgaagcag gtaaccgtcg agaacttcaa gaaccgcgta ctgatgtccg
     3601 ccgacctgga ggagcacctg gagcatatcc agcactacgt ggatctcggc ttcgacgagg
     3661 tctacgtaca caacgtcggg cgcaaccagg agcagttcat aagagcatac ggcgagcgca
     3721 tcatccccaa gctccgctgg ccggagaggt gatctccgca gcggatataa tgcaggaggt
     3781 tatggactgg cgagaccaca taaactcgga ccctgagata ctatccggca aaccggtcgt
     3841 caaggggacg cggctttcgg tagagttcct gctggggttg ttcgccgtgg gatggactaa
     3901 ggagcaggtc ctcgaaaact accccacgct cgactcggaa gccataagag ccgttttcgc
     3961 ctttgccgcc gaacatgttc gcgaggagag tgtttcgctg ggaacccgcg gttgagatgc
     4021 ggtagccgcc ttgcgcttcc tcgccgacga aaatttccca ttgcgaggcg tcggctttct
     4081 cacgaacgct ggacacgagg tcgcggccat catactcgag tcgccgggcc tgcctgatga
     4141 gcaggtcatg ttaagggcgg ctcgcgaaga ccgcgtactg ctaacggtcg acagggatca
     4201 cggaagactc ctctatcgaa ccggcgtggt ctactttcgc tttgatccag cctatccgga
     4261 ggaacccgta gagcatctgc tctcgatcct cgatcagccc gagacttctg tcctcggcat
     4321 gctcaccgtc atggagcgcg accgggtgag acaaagaccg ctgcccggcg cagaccggac
     4381 atagtctgtg cgacccggtg atgagaaggt ggatctcggc gagcgcgtca tctccaagct
     4441 ccgctggccg cagtagtaga aaatgaaggt cgggcaagag gtcaacacgg aaatcgtggg
     4501 cctatgatcc actaccattg tggtagcggg gtgacgaatt gaaagtcgcg gtcgtgggag
     4561 caggcataat cggctcttcc gttgcctacc ggctctcgga aggcggagcg aaggtcgttc
     4621 ttatcgacgc cgctgagcct ggctccggca ccacctcggc cagcttcgcc tgggtcaacg
     4681 ccaacaacaa gctcccccgt gagtacttcg agctgaacct tgccgggatg cgcgagcacg
     4741 agcagctccg cgatgagatc ggcggcggat ggcttcaccc taccggaaac ctgatccaga
     4801 ccacggacga aggacaggaa gagctcgaaa aacgcgtcgg gcggcttcgc tcctggtcct
     4861 acgccgccga gatgctcacc gcttccacgg tcaacgagaa gctcgaacct gaggccgttt
     4921 tcccgaaccc agagaccagg atcgcttact tccccgaaga atcctgggtc gacgccccag
     4981 cccttacgag aacgctcgtg caagccgcca ggcgcaacgg agcactgacg ctggtcggaa
     5041 acgcggtaca cggcatagag gtcggaggcg agggtgtgac actgcgactg gagaaaggcg
     5101 atgccgttca cgccgacgcc gtggtcaacg ccaccggcgc gggggccgca tccgtggcgg
     5161 agatggtcgg gaggaagttg ccgctcgacg tgttccgggg tctgctcgtc cgggttgccg
     5221 tccccggcga cccgttagga cgcctcatac acacgccgcg cgtcaacttg cgccccgacg
     5281 gtcccggcta cgtcttgctg aaccacgatt cggtggacgc aaagcttacc gacgacttct
     5341 cagggacgga agacccgctt tgcgcggagc ttctggagcg tgcgcgacgc gtatttccgg
     5401 cgctcgctga ggctgaagtc gtggaggcga ggttcgggct tcgtcctgtc ccggcggatg
     5461 ggcattcgtg tgtgggtgag ctctccgaga ttcctggcta ttacgaggcc gtgactcaca
     5521 gcggtgtcac gctcggaccg ctcgttgggc ggttgctggc gcgtgagatc ctgaccagcc
     5581 aggtggaccc gctcatcgcg ccgtttcgtc cggatcgctt cccatcaacg taagcccgga
     5641 gctcgctggt gctgtttaca acccgtctcg ccgggtccta tactgacgcc cgaggaaagg
     5701 gaggcgagga cgcacgctat ggcttttgca cagcaagggt ttctcgttgc ggagggagcg
     5761 gtgctccccc tggacaaacc accgtcgtag cgtgggctgg atcttgcccc actggtctga
     5821 tgcgggatca cggaccgctc ttcatagtgt cgcaggcgga ccgccctcat gcgcgctagc
     5881 ttccggtcta cgaaaggagt agaagctatg gcaaggacac tttttagcgg gggaacgata
     5941 gtaaccgcag aaggcagcta cagggccgac gttctcgtcg aggacgagaa ggtagtcgcc
     6001 gtaggcacga acctcgacgc ggacggcgcc gaaactgttg acgcttccgg caaactggta
     6061 atgcccggct ttatagacgc ccacacccac atggacatgc ccttcggggg cacggtcacg
     6121 gctgacgact gggccacggg cacggaggcc gcggcggcgg gcggaactac catgatcatc
     6181 gacttcgccc tgcaagaggc aggtggcacc ctcgccggag ccttcgatac gtggacggag
     6241 aaggcgcggg acaaggcggt catagattac agcttccacg tcgccatcac tgacctgcgc
     6301 gacgatata
//
LOCUS       DATAIR010000700         8173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_211842, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000700 DATAIR010000000
VERSION     DATAIR010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211842"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VHM69_16560"
     CDS             <1..1166
                     /locus_tag="VHM69_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2742055.1"
                     /translation="SRVRDLFKQARENAPAIIFIDELDSIGRARGQSAIGGSSEQEQT
                     LNQILTEMDGFSSKEGVIVLAATNQPDVLDKALLRPGRFDRRVVVNLPDKVGREAILK
                     VHTRSVPLGQDVDLGELAAATPGLSGADLANLVNEAALLAARREQEEVRQKDFLDALE
                     KIVLGPERPLLMSPEERERIAYHEGGHAILGLVVPGADPVNRVTIVPRGQALGITYQR
                     PEDDRYNYPEGYLRARIIGALGGRSAEEIVYGTRTTGAENDIEQATELARRMVTRWGM
                     SDKLGTVELAPRENPYLAGGGGGYPGEKPYSEETARAIDEEVLRIIDESHEDARRLLT
                     QHRAELEALAQTLLERETLDEAEILQVTGLAPAPRLENAKIAAPSSNSRRDAR"
     gene            1313..1780
                     /locus_tag="VHM69_16565"
     CDS             1313..1780
                     /locus_tag="VHM69_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2742056.1"
                     /translation="MSVFPTTVLLATDGSEDAKLATTTAVELANSTGSELHVVFVTQL
                     PSVPGAVGYWSGVPTGYPYTDPELEPDIEQQGRKLLDAEVEEVRSAGGRVAEAHLKIG
                     DAAAEIVALAEDIGAGLIVMGSRGLGGIRGALMGSVSEAVVKHAHCPVLVVRH"
     gene            1844..2518
                     /locus_tag="VHM69_16570"
     CDS             1844..2518
                     /locus_tag="VHM69_16570"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2742057.1"
                     /translation="MADENVVLVTFEEESRAYQAASVLKKASAEGRIYLHAVAVVQRT
                     EDGTLRVKEGDADDFPVGTWTGGVIGGTTGGIIGLTLGTLGGPLGLLLGGAGGALLGS
                     LIDLDTAEEAESVLATMARVIEPGKTALIAHVTEPAVKVIDGEMERPGGEVIRRPVDE
                     VEAEIAAVDEAARAAEAEARRKIREQKAAERKEKVDEKIESLKAKWNTYGSLWPTLSS
                     RINDDR"
     gene            2781..3734
                     /locus_tag="VHM69_16575"
     CDS             2781..3734
                     /locus_tag="VHM69_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2742058.1"
                     /translation="MRSGSFKIGRAFGIDVRVHWTFFLLLFYFAFVGYRGAGNLVGAL
                     ATVAVIVALFACVLLHEFGHSLVAQRLGIEIPDITLLPIGGVARFKTLPAKPMDEVKI
                     AVVGPLVNVVLAPIFFGMGIALGADPLTMGSPIGGFVSAGRFFVYLGYINVALVLFNM
                     LPAFPMDGGRVLRGLLASRLGAVRATDIASVVGQHFAAVFLVLGILGGNFILVLIALF
                     IFFGGPNGGRDGAPEGANVRPCRAHRHGRPPPEGGLSGTVAAILFLRRCRPYPGRPSE
                     KRDEPEAGGRVVSGEAARGRPARRRTGGRFKQTTWRTSNAP"
     gene            3747..5024
                     /locus_tag="VHM69_16580"
     CDS             3747..5024
                     /locus_tag="VHM69_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2742059.1"
                     /translation="MTPDGPREDGAPEETVGARDTSHKVTRAEPGTDAQTGPNRAPLA
                     ESDGSVPSGAVVWVRISPSQVTSTVVVALLTAAVVCGAFFVLWRVRTFVGWFVIALFL
                     AAVLNPVVDWLQRRHRLIKRPLAIVLAYLGLVVALLFVVGLLLPVLADQIDSLIKFVA
                     AVANAPEGPTEYLMGLARQYGFGWVFERFSELLGDVRSQMGVAARNFLSSTGDVVAGA
                     AGFAAALGTVLTLAFLLLLGSERYINAGVGLFAEAHRPLVRRILARSAGALTGYVTGN
                     LAISAICGVTTFVVLVVLGMPYAAALALLVAVPDLIPLAGATLGGALLVVVGLFVAPW
                     KAAVLLVYIVVYQRVEDSILQPLVYSHAVQLNALAIFIAILVGGILLGIPGALVAIPV
                     AQIIRIVVTEILAYRRAKEEANEPVSSPSRPTS"
     gene            complement(5114..7066)
                     /locus_tag="VHM69_16585"
     CDS             complement(5114..7066)
                     /locus_tag="VHM69_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX2742060.1"
                     /translation="MSGPTTASKRPVQRFKGWLLADVVEDVPGPEAKEGRSEQHPWWQ
                     VVCLTGVDYFSTLGYIPGIAALAAGALSPIATLFIVGLTLFGALPMYRRVAEESPHGQ
                     GSIAMLEDLLSFWRGKLLVLVLLGFVATAWIVTITLSAADAAVHVAENPLVPSFLHGR
                     EVLITLALLAFLAAVFLRGFKEAIGIAVFIVGVYLLLNLVVVGVGFYEIATHPRSVVS
                     WQNALFENYGNPLVMVVASLLVFPRLALGLSGFETGVGMMPLVRGDEGDDPERPEGRI
                     RNTRRMLTVAALTMSFYLLATSLVTVMLVPSREFEPGGAANGRALAYVAHDQLGEVFG
                     TVYDLSTITILAFAGASAMAGLLNIVPRYLPRYGMAPEWARAIRPLVLLYTAVSVAIT
                     FVFSADVDAQAGAYATGVLAMMTSAAFAVTLSTWRRGSRLPAIFFGLVSVVFVYALVA
                     NEIQRPDGLVIASFFIVTMIATSLVSRVYRSLELRQERIDMDETAERFIEAASHRGEI
                     HIVANRRQAGDEAEYDNKEKEQREDNHIPKEVPIIFLEVDVEDASEFEDVLEVRGVEV
                     GGHKVLRAKSSVVPNAIAALLLHLRDATGRAPHCYFGWTEGNPIVYLFRFLLFGEGDT
                     APVTHEVLREAEPDGTRRPAVHVGGR"
     regulatory      complement(7136..7300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            7492..>8173
                     /locus_tag="VHM69_16590"
     CDS             7492..>8173
                     /locus_tag="VHM69_16590"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2742061.1"
                     /translation="MRNGSTQQMKMTAGMDLGDKYSHLCLIDTESGELIEESRLRTTP
                     EAMRRRFDSQRPLRVAIEVGTHSPWVSRLLEECGHEVLIANPRKTRLIYGERRKTDKL
                     DAEKLARLARADPELLYPVEHRGCDSQDHLAIVHSREALVRSRTQMINHVRGTVKSFG
                     YRLPKCSTPSFHKKVPGQLPSELAEVLGPIVETVGSLTEKIKDYDRRIEELAAERYPE
                     TKLLRQVPG"
BASE COUNT         1512 a         2553 c         2741 g         1367 t
ORIGIN      
        1 ccagccgcgt cagggacctc ttcaagcagg cgcgcgagaa cgccccggcg atcatcttca
       61 tcgacgagct cgactccata ggccgcgccc gcggccagtc ggccatcggg ggatcgagcg
      121 agcaggagca aaccttgaac cagatcctca ccgagatgga cggcttctcc agcaaggagg
      181 gcgtgatcgt tctggccgcc accaaccagc ccgacgtgct cgacaaggcc ctcctgaggc
      241 cagggcgctt cgaccggcgc gtggtggtga acctccccga caaggtgggc cgcgaggcca
      301 tcctcaaggt ccacaccaga agcgtgccgc tcgggcagga cgtggacctt ggggagctcg
      361 cggcagcgac gcccggtctc tcgggcgccg acctggctaa cctggtaaac gaggctgcgc
      421 tcctggcggc gcgccgcgag caggaggagg tgcgccagaa ggatttcctg gacgccctgg
      481 aaaagatagt gctaggcccc gagcgaccgc tcttgatgag cccggaggag cgggagcgca
      541 tcgcctacca cgagggcggc cacgccatcc tcggcctcgt cgtgcccgga gccgatccgg
      601 taaaccgggt gacgatcgtg ccgcgtggtc aggccctggg catcacctac cagcgccccg
      661 aggacgaccg ctacaactac ccggaagggt acctcagggc gaggatcatc ggcgccttgg
      721 ggggacgcag cgcggaggag atcgtctacg gcaccaggac caccggagcc gaaaacgaca
      781 tcgaacaagc caccgagctc gctcgtagga tggtcacccg ctgggggatg agcgataagc
      841 tgggaacagt agaactcgct ccccgcgaga acccttacct cgcaggcgga ggtggaggct
      901 atccgggcga gaagccctac agcgaggaga cggcccgggc catagacgaa gaggtcttgc
      961 gcatcattga cgagagccac gaagatgcca ggcgtctgct cacgcagcac cgtgcggagc
     1021 tcgaggcgct cgcccagaca ctactcgagc gcgagaccct ggacgaggcg gagatccttc
     1081 aggtcacggg actggccccc gcgccgcgcc tggagaacgc gaagatcgcg gccccgagct
     1141 cgaactcgcg cagagacgcc aggtagggat ctccgcgatg cccactccca aggatgaatg
     1201 ccgcttaagg aggaggacga tagctggtaa ggcttagggc ccttaaggat gactcactct
     1261 cttgatgtca ggtggatgat cgccgaagaa atgtccccag gaggcgtagc cgatgagtgt
     1321 cttccccaca accgtacttc tggcaaccga cggctcagag gacgccaagc ttgccaccac
     1381 aaccgctgtc gagctggcca acagcaccgg ctccgaactg cacgttgttt tcgtaacgca
     1441 gttgccttca gttccgggcg ctgtgggcta ctggtcgggc gttcccacag ggtatccata
     1501 caccgacccg gagctcgagc ccgacattga gcagcaaggt cgtaaactgc tcgatgcgga
     1561 ggtggaggaa gtacgctcgg cgggaggcag ggtcgcagaa gcacatctaa agatagggga
     1621 tgcggccgcg gagatagttg ctttggcgga ggacataggg gcggggctca tagtgatggg
     1681 tagccggggc ctagggggga taaggggcgc actcatgggc agcgtctcag aggccgtggt
     1741 caagcacgcc cactgccccg tcttggtagt acgccattaa acagaacctg agtgggctta
     1801 tccaaccatc aagtgaggaa gcgcaaaaga aaggagcagg gccatggccg acgagaacgt
     1861 ggtgctcgtg acgtttgagg aagagagcag ggcctaccag gcggcgagcg tcctcaagaa
     1921 ggcaagcgcg gaggggcgca tctatcttca cgcggtggcc gtcgtgcaga ggacggaaga
     1981 cggcacgctc agggtcaagg agggcgacgc cgacgacttc ccggtgggca cctggaccgg
     2041 aggcgtcatc ggcggcacga ccggtgggat aatcggtctc accctgggca cgcttggcgg
     2101 accccttggg cttctgctgg gcggcgcggg cggcgcgctg cttggctcgc tgatcgacct
     2161 cgacaccgct gaggaagcgg aaagcgtcct ggccacgatg gccagggtca tcgagccggg
     2221 caagacggcc ctaatcgcgc atgttacgga gccggcggtc aaggttatcg acggcgagat
     2281 ggaacgtccg ggaggtgaag ttatccgtcg tccggtcgac gaggtggagg ccgagattgc
     2341 cgccgtcgat gaagccgcca gggccgccga ggcggaagcg cggcgcaaga tcagggagca
     2401 gaaagcggcc gagcgcaagg agaaggtcga tgagaagatc gagtcgctca aggccaaatg
     2461 gaacacctat gggtctcttt ggccaacgct ttcgagtcgc atcaatgacg atcgctgatg
     2521 ccacagtagc tcgaggcgcc cacccaggca ctactggagc gggagacact cgaggacggg
     2581 gtggagaccc ttcgggccac gggaccggcc cccgccccgc gcccggagaa cgtgaagatc
     2641 gcggagctcg gactctcgca gggacgccgg ctccaggcaa gtcccaaatt tggtcggcgc
     2701 atggcacaca ctgctcgaga taacccgtct catcggtgcc atggtcggga cctaagctcg
     2761 agcggccgaa aggaaccacc atgagaagcg ggtccttcaa gatcgggcgc gcgttcggta
     2821 tagacgtaag ggtccactgg accttcttct tgctcttgtt ctacttcgcc ttcgtcggat
     2881 accggggagc tggcaatctg gtcggggcac tcgccaccgt cgccgtgata gtggcgctct
     2941 tcgcctgcgt gctgctacac gaattcgggc actccctcgt ggcccagcgc ctcggcatcg
     3001 agatccccga cataaccctc ctgcccatag ggggcgtcgc caggttcaag accctgccgg
     3061 caaagccgat ggacgaggtc aagattgccg tcgtcggtcc cctggtgaac gtggtactgg
     3121 cccccatctt tttcggcatg gggatcgcgc tcggcgccga tccgcttacc atgggtagcc
     3181 cgatcggggg tttcgtctcg gccgggcgct tcttcgtcta ccttggctac ataaacgtcg
     3241 ccctggtgct cttcaacatg ttgccggcct tcccgatgga cggcgggcgc gtattgcggg
     3301 ggttgctcgc gagccgcctg ggagccgtgc gggctacgga tatcgcctcg gtggtcgggc
     3361 agcacttcgc cgctgtcttc ctggtgctcg gaatcttggg cggcaacttc atcctggtcc
     3421 tgatagccct cttcatcttc ttcgggggcc cgaacggagg ccgagatggt gcgccagagg
     3481 gagccaacgt gaggccttgc cgtgcacacc gtcatggccg cccacccccg gaggggggtc
     3541 tgagcggcac ggttgccgcg atacttttcc ttcgtcgctg tcggccttat cctggccggc
     3601 cttcggagaa gcgggatgag ccggaagcgg gtgggagggt ggtgagtggt gaggcggcgc
     3661 gcggacgccc ggcgaggcga cgaacgggcg ggcgattcaa gcagacgacg tggcgcacca
     3721 gcaacgcgcc gtaaggagac gcggacatga cgcccgatgg gccccgcgag gatggggccc
     3781 ccgaagaaac ggtgggggcc agagatacga gccataaggt gacgcgggcg gagcccggta
     3841 cggacgcgca aacggggcct aatagggctc cacttgccga atccgacggc tccgtgccga
     3901 gcggggccgt tgtgtgggtg cgtatctcgc cctcgcaggt gacgagcacg gtggtcgtag
     3961 ccctcctgac cgctgcggtg gtttgtggcg cgttttttgt gctctggcgg gtgcgcacct
     4021 ttgtcggctg gttcgtcatc gcgctgtttc tggcagcggt gctgaacccg gtggtcgatt
     4081 ggctccagcg gcgccacagg ctgatcaagc gccctttagc catagtgctc gcctaccttg
     4141 ggctggtggt ggcgctgctg tttgtggttg ggctactctt gcccgtactg gccgaccaga
     4201 tcgacagcct gatcaagttc gttgccgccg tggccaacgc ccccgagggg ccgaccgaat
     4261 acctaatggg gctcgcccgg caatacgggt tcggttgggt attcgagagg ttcagcgagt
     4321 tactgggtga tgttcgcagc caaatggggg tggcggccag aaacttcctc tcatcgaccg
     4381 gagacgtggt agccggcgcg gccgggttcg ccgccgcgct gggaaccgtc ctgacgctcg
     4441 cgttcttgct gctcttgggc agcgagcggt atatcaacgc cggggtaggg ctcttcgccg
     4501 aggcacaccg gccgctcgtg aggcgaatcc tcgcgcgctc ggccggggca ctcacgggct
     4561 acgtcacggg caacttggcc atcagcgcga tctgcggcgt aacgaccttc gtcgtgctgg
     4621 tggtccttgg catgccctac gccgccgctc tggccctcct ggttgcggtg ccggacctca
     4681 taccgctggc cggcgcgacg ctgggcgggg cgttgctcgt cgtagtgggg ttgttcgtcg
     4741 caccatggaa ggccgcggtg ctgctcgtgt acatcgtggt ctaccagcgg gtggaggata
     4801 gcatcctcca acctctcgtt tacagccacg ccgtccagct caatgcgctg gcgatcttca
     4861 tcgccatcct ggtagggggg attctgctgg gcattcccgg ggcgctcgtg gccatcccgg
     4921 tagcccagat catccggata gtggttaccg aaatcctggc ttaccggcgt gccaaagagg
     4981 aagcgaacga acccgtctcc tcgccgtcac ggcccaccag ctgagggctg agtgcttata
     5041 gctcgcacac cggctcggtt ccatcgcagt ccgccgccag acggtttctt gacggagtct
     5101 aaagggggtc cgatcatctg ccgccgacgt ggacggccgg gcgcctcgtg ccgtctggtt
     5161 cggcttcgcg caagacctcg tgggtgacgg gtgcggtatc cccctccccg aataggagga
     5221 agcggaacag gtacacgatc gggttgccct ccgtccaacc aaagtagcag tgcggggccc
     5281 taccggtggc gtctcgaagg tgtagcaaga gggcggcgat ggcgttcggg accaccgagc
     5341 tcttggcgcg cagcaccttg tgaccgccga cctccacgcc cctcacctcc agcacgtcct
     5401 cgaactcgga ggcgtcctcc acgtccacct ccagaaatat gatgggtact tccttgggaa
     5461 tgtggttgtc ctcgcgctgc tctttctcct tgttgtcgta ttcggcctcg tcgcccgcct
     5521 ggcgacggtt ggcgacgatg tggatctctc ccctgtggct ggccgcttcg atgaaccgct
     5581 cggccgtctc gtccatatct atcctctcct gcctcagctc caacgaacgg tagaccctcg
     5641 agacgagcga cgtcgcgatc atcgtgacga tgaagaagga cgcgatcacg agcccatcgg
     5701 gccgctgtat ctcgttggcg accaaagcgt atacgaagac gacactcacc agaccgaaga
     5761 agatcgccgg caacctcgag cccctgcgcc aggtcgaaag cgttaccgcg aaggcggcgg
     5821 aggtcatcat cgccaagacg cccgtggcat aggcgccggc ctgggcgtcc acgtcggcgg
     5881 agaagacgaa ggtgatcgcc acggagaccg cggtgtacag gagcacgagg ggccttatcg
     5941 cccgcgccca ctcgggcgcc atgccgtagc gcggcaagta gcgcggcacg atattcagga
     6001 gcccggccat ggccgaggcc cccgcgaacg ccaatatcgt tatcgttgag aggtcgtaga
     6061 cggtgccgaa gacttccccg agctggtcgt gcgccacgta ggcgagggcg cgcccgttgg
     6121 ccgctccccc gggctcgaac tcccgagagg ggaccagcat caccgtcacc aggctcgtgg
     6181 cgagaaggta gaagctcatg gtgagcgcgg cgacggtcag catcctgcgc gtgttgcgga
     6241 tgcgcccttc cgggcgctcg ggatcgtcgc cctcgtcgcc gcgcacgagc ggcatcatgc
     6301 ccacccccgt ctcgaagccg gagagaccca gcgccaggcg cgggaacacg agcagcgagg
     6361 cgacgaccat cacaaggggg ttgccgtagt tctcgaacaa agcgttctgc cagcttacga
     6421 cgcttcgggg atgggtcgcg atctcgtaga agcccacgcc cacgacgacg aggttcaaga
     6481 gcaagtatac cccgacgatg aagacggcga tgcctatggc ctccttgaag cccctgagga
     6541 agacggcggc gaggaacgcc aggagcgcta gcgtgatgag gacctcccgg ccgtgcaaga
     6601 atgaaggcac cagcgggttc tccgcgacgt ggacggcggc gtcggcggcc gagagggtga
     6661 tggtcactat ccaggccgtc gcgacgaagc ccaggagcac gagcacgagc agcttgcccc
     6721 tccagaagga gagaagatcc tcgagcatcg cgatcgagcc ttgcccgtgc gggctctcct
     6781 ccgcgacgcg cctgtacata ggaagcgccc cgaagagcgt tagacccacg atgaagaggg
     6841 tggctatggg cgagagcgcc ccggcggcga gcgcggctat acccgggatg tagcccaaag
     6901 tggagaagta gtcgacgccc gtcaggcaca cgacctgcca ccacgggtgc tgctcggagc
     6961 gcccctcctt ggcctccggg ccgggcacgt cctcgaccac gtccgcgagc agccaaccct
     7021 tgaaccgctg gactgggcgc ttggacgccg tcgtcggccc gctcatcggg gccgacccac
     7081 gagacggcag gggtcggcag gacgggagac tcgtagggat cgtcgcgtga gcacgtgtca
     7141 ccttcttccg cgcctgcggg gttagccgtc aggttagggc cgaaagagct atgccctact
     7201 ccagcggtca ccgcccgccg gcgatgcacc ccgaggaccc aatgcgtcct cggtgggtcc
     7261 ccacccccgg tgccacccgg gggctcggcg tcttcttggc atcgtgttgg cacccggcgg
     7321 tatacgcctc gctcgcacga ttgtcaatgt tctcgaccgt catattcacc cgagtgcatg
     7381 gacctcgact ttagatgaga tgagaaggcc gatcctccgc gcagtaccct gtcggagggt
     7441 ctgcaaataa tcgacgggct cttttagtgg cccagagagg aggatcggcc aatgcgtaat
     7501 ggtagcaccc agcagatgaa gatgacggcg ggtatggatc tgggcgacaa gtacagccac
     7561 ctctgtctca tcgacaccga aagcggcgaa ctcatcgaag agagccggct gcgcaccacc
     7621 ccagaggcga tgcggcgccg gttcgattca caacggccgc tgcgtgtcgc aatcgaggtg
     7681 ggcacccact cgccctgggt aagccgtctg ctggaggagt gtggccacga ggtactgatc
     7741 gctaatcctc gtaagactcg gctgatctat ggcgaaaggc gtaagaccga caagctcgat
     7801 gccgagaagc tggcccgcct cgctcgggcc gatccggaac ttctgtaccc ggtggagcac
     7861 cgaggctgcg actcccagga tcacctggcg atagtccatt cgcgcgaggc gctggtccgt
     7921 agccgcaccc agatgatcaa ccacgtgcgc gggacggtga aatctttcgg ataccgcttg
     7981 cccaagtgct cgacgccgag cttccacaaa aaggtcccag gccaactacc ctccgagctt
     8041 gccgaggtcc tcggacccat agtagagacc gttggctcgc tcaccgagaa gatcaaggat
     8101 tatgaccggc gcatagaaga gttggctgca gaacgttatc cggagacgaa gcttttgcgg
     8161 caagtgccag gag
//
LOCUS       DATAIR010000701         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_162644, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000701 DATAIR010000000
VERSION     DATAIR010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162644"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VHM69_16595"
     CDS             <1..1132
                     /locus_tag="VHM69_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2742062.1"
                     /translation="ALQGLTTLKVFGGSADESERVAAASEEFRNRTMKVLRYAFLSGF
                     VLEFMTAAAIGLVAVTLGVRVISGNMPFEAAFLVLLLAPEFYKPLRELGVHRHAGMEG
                     SAAADRIFEILSTPVPVRQGSGAHGLVTHGLAIEFSGVGYTYPGSDHAALSDLTFTLE
                     AGTRTALVGRSGAGKSTMVNLLMRFVDPESGTIRANGVEITDLSAMTWRKSVALVPQR
                     PHLFYGNVLENIRLARPEASTEEVERAAELVGAAEFIRRLPDGYSTEIGERGARLSGG
                     EAQRIAIARAFLKDAPVLVMDEPTSSLDPESERLVQSALDRLARDRTVLVIAHRLNTV
                     YKADRIAVLDDGRLVETGTHRELIQRGGLYSSLVNAYGRVPA"
     gene            1129..>2404
                     /gene="cydC"
                     /locus_tag="VHM69_16600"
     CDS             1129..>2404
                     /gene="cydC"
                     /locus_tag="VHM69_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEX2742063.1"
                     /translation="MRTFARLVGFLKPHFPRVALAVLLGVATVASNVGLLATAAYVIS
                     AAALVSFLGALVIPIYLVRLFGVSRAGARYAERMVSHDLTFKLIADFRTWFYTRLEPL
                     APARLLSYRTGDLLSRIVQDVDELQNLYLRIVAPVAVASLTSLLSFVLLYAFDPTLAF
                     VALGFLLATGIGVPLLVRSLARGLGRKQLELRGELNARIVDGIQGAQDLLAFGREEDQ
                     GRAIATLNRRLGRLQGRMAFITGLQDALCDVMMNLAMLAMLVLAIPLVAGGEIRGVYL
                     AFLALVVLGSFEAVQPLGTAFQFLGRSVRAGERLFEIVDAEPEVKDPAEPLPLPENHK
                     LVVESVSFLYEAGGPHVLEDVSFTLEPGKRVAVVGPSGAGKSTLAGLILRFRDPTSGT
                     ISVGGNDVRGYAQEDLRALIGVVAQDTHVFNET"
BASE COUNT          390 a          783 c          783 g          448 t
ORIGIN      
        1 tgccttgcag ggactcacta ccctcaaggt cttcggcggg agcgccgacg agagcgagag
       61 ggttgcggcg gccagcgagg agttcaggaa ccgcacgatg aaggtgttgc gctacgcttt
      121 cctctccggg ttcgtgctcg agttcatgac cgcggcagcg ataggtctcg tcgccgtcac
      181 gctcggggtg cgtgtgatct ccgggaacat gcccttcgaa gctgccttcc tggtgctgct
      241 cctggcaccc gagttctaca aacctctgcg cgagctaggc gtccaccgcc acgccggcat
      301 ggaggggagc gccgccgccg accgcatctt cgagatcctg tccacccctg tgccggtgcg
      361 gcagggttcg ggcgctcacg gactcgttac acacggactt gccatcgagt tctccggcgt
      421 cggttacacg tatccgggta gcgaccacgc ggcgctctcg gacctcacgt tcacgctgga
      481 ggcagggacc cggaccgcgc tcgtcgggcg cagcggcgcg ggaaagagca cgatggtgaa
      541 tttgctcatg cgcttcgtcg atcctgaatc aggcacgata cgcgcgaacg gcgtggagat
      601 cacggacctg tccgcaatga cctggcggaa gagtgtggcg ctcgtgccgc agaggcccca
      661 cctcttctac ggaaacgtgc tggagaacat ccgcctcgca cggcctgagg cgagcacgga
      721 ggaggtcgag agggcggcgg agttggtcgg ggccgccgag ttcatccggc gtctccctga
      781 tggctattcg actgagatag gggagcgtgg ggcgcgtctg agcgggggag aggcccagag
      841 gatagccatc gcccgcgcct tcctcaaaga cgcacccgtg ctcgtcatgg atgagccgac
      901 ctccagtctc gacccggaga gcgagcgcct cgtgcagagc gccctcgacc gcctggcccg
      961 agaccgtacg gtactcgtga tcgcccaccg cctcaacacg gtatacaaag cggacaggat
     1021 cgccgtcctc gacgacgggc gtctcgtcga gacagggacg caccgcgagt tgatccagcg
     1081 cggcggactc tactccagtc tcgtgaacgc ctacggaagg gtgccggcgt gaggaccttt
     1141 gcgcggctcg tcggcttcct gaagccacac ttcccccggg tggcgctggc agtcttgctc
     1201 ggcgtcgcga ccgtggccag caacgtcggg ctgctcgcta cggcggcgta tgtgatctcc
     1261 gccgccgcgc tggtctcttt cctgggcgct ctggtcatcc cgatctacct cgtacgtctc
     1321 ttcggcgttt cgagggcagg cgcgcgctac gcggagcgca tggtctccca cgacctgacg
     1381 ttcaagctga tcgcggattt cagaacgtgg ttctatacgc gcctcgaacc cctcgcccca
     1441 gcccgacttc tgagctaccg aacaggggat ctcctctcac gcattgttca ggacgtcgac
     1501 gagctgcaga acctctacct gcggatagtc gctcccgtgg ccgtggcctc gttgacttcc
     1561 ctcctgtcct tcgtgctcct gtacgcgttc gacccgacgc tggccttcgt cgccctcggg
     1621 tttctgttgg ccacaggcat tggggtgcca cttctcgtca gaagcctggc gcgcgggctc
     1681 ggacggaagc agttggagct gaggggcgag ctgaacgccc ggatcgtgga cggtatccag
     1741 ggcgcgcagg acctgctcgc tttcggcagg gaggaggatc aaggacgtgc catcgccacc
     1801 ctgaaccgca ggctgggccg tttgcagggg aggatggctt tcattacagg acttcaggac
     1861 gctctctgcg acgtgatgat gaaccttgcg atgctcgcga tgctcgtcct ggcgatccca
     1921 ctcgtcgcag gtggggagat ccggggcgtc tacctggcct tcctggcgct cgttgtactc
     1981 ggtagcttcg aggccgtgca gcctctcggc acagccttcc agttcctcgg acgttccgta
     2041 agggccggag aacgtctctt cgagatcgtg gacgcagagc ccgaggttaa ggatcccgca
     2101 gagccgctcc ctctacccga gaaccacaag ctcgtcgtcg aaagtgtgag cttcctctac
     2161 gaggctggag gcccacacgt cctcgaagac gtctccttca ccctggaacc gggcaaacgg
     2221 gtcgctgttg tcggcccgag cggagcgggg aagagcacgc tcgccggtct catcctgcgc
     2281 ttccgggatc cgacgtcggg tacgatatcc gtcggcggca acgacgtacg cgggtacgcc
     2341 caggaagact tgcgggccct gatcggggtc gtcgcccagg acacccatgt cttcaacgag
     2401 accc
//
LOCUS       DATAIR010000702         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_349720, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000702 DATAIR010000000
VERSION     DATAIR010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349720"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VHM69_16605"
     CDS             <1..663
                     /locus_tag="VHM69_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2742064.1"
                     /translation="PKDILANAGTYGPLTIEAPEGSLVNATYPSAVVAGNVETSNRIA
                     DTVLAALSGFAPQGIPAQGQGTLNNTIIGGRGWTYYETVGGGQGASHRGPGPSGVHIG
                     MSNTLNTPTEAFEMEYPMRVEHYELLYGSGGEGGHLGGDGIVRSVKVLEPASLSLLTD
                     RRRHGPRGVEGGGPGRVGRNLLNGEELPPKVSRELAAGDVVTVETPGGGGYGKPPNSQ
                     SP"
     gene            759..1949
                     /locus_tag="VHM69_16610"
     CDS             759..1949
                     /locus_tag="VHM69_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX2742065.1"
                     /translation="MPDRPHQHGMSRRRFVKDVGLGVAGLSMPGLLACRGAVSAQTVG
                     SDALHRRVRAELRKFTDWLDQNGVDGYIGEVGWPDDYAGDAALWNSLANAWYGDAAGL
                     WVTQWSTGEWWGTDYKLAAYEDRHSPNGVDSANTQAVVTEAHPTTPSYLRGVNVSGAE
                     FGAPFSLDATSNFSNRNPGRYDTAYHYDGQATFDYLASRGIRLIRLQFRWERIQPTPG
                     GPLDGTELRRLKAAVRRATGAGLEVILDVHNFGDYYIERNGRGVRFPIGSDRLPISRF
                     ADLWRRLSNNFKAMPGIVGYGLMNEPAGMPRVGDFSPAKVWEKASRRALDAIRSNGDL
                     SLVLVQGYQWAGAQDWPTNHPASWIDDHNFRYEAHHYWDRNNSGAYSNSYAEEVAEAR
                     SRGY"
     gene            1971..2429
                     /locus_tag="VHM69_16615"
     CDS             1971..2429
                     /locus_tag="VHM69_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2742066.1"
                     /translation="MQGVVESIYVTGEGSASMERVDEVRTIEGCGIEGDRYCEGMGYW
                     TNFGDVCEVTLISSEDLEHIERELGIGVGNGEHRRNIVTRGVSLKVLRGKRFRIGDAV
                     LEYDGPRPPCRHVQDLTEPGMTRALKGRGGICARVVQAGRIRARDAIEIQ"
     gene            complement(2433..3734)
                     /locus_tag="VHM69_16620"
     CDS             complement(2433..3734)
                     /locus_tag="VHM69_16620"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX2742067.1"
                     /translation="MKSFLSHDRSWAYPSMLLITVCAVALGVWARLYHLGFPPKIMWD
                     EKYFPAMANNFLDGVYQFDLHPPLGKFILAVGIFFLGDEPLGWRIMPTLFGLALLPLV
                     AVLGWYCLRERVAAILMLTFFAVETMFIVHSRTGVMDIFVVFFVLATFLAALRAETGR
                     QVIWTSVFLGLAIAVKWAAFPVAIPAGYVLWRKGLLRPFLAGLWISAVIYIAVIYVGA
                     LISVTMNPVEAWVWTWNWHVHAAQKITVAVDHPWGSPWWSWPIMLRPIRFFYGVNAES
                     LLQVVVAIGNPLIWWSSTLAVLAGAFDIARKAIARKFSADDPIIPIVLGYFFLLAPWV
                     PGTRIPYIYNYLPIYPFAILALVYWLCRIWNRPQWGPWVVVGFTVCAVALTLFFLPMV
                     IALPMSKDALMDRVWTDYWLYQNDPVPGSGCLRENPGELCG"
     gene            complement(3801..>4100)
                     /locus_tag="VHM69_16625"
     CDS             complement(3801..>4100)
                     /locus_tag="VHM69_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742068.1"
                     /translation="GLILPATLLAFGAGWGWPGLFNFAVVKTNPGAPAAATGITQTGA
                     SGGAALGPLVFGLVVEATSYGTAWLVSGVIALLALVAILMGRAMILRDREPRFGA"
BASE COUNT          845 a         1280 c         1342 g          633 t
ORIGIN      
        1 cccaaagaca tcctcgccaa cgccggcacc tacggtcccc tcaccataga agcccccgag
       61 ggcagcctcg tcaacgcgac ctacccatct gcggtcgtcg ccgggaacgt cgagacctcc
      121 aaccgaatcg ccgacaccgt actcgccgcg ctctctggct ttgcgccgca ggggataccg
      181 gcgcagggcc agggtactct gaacaacacg ataatcgggg ggcgtgggtg gacctactac
      241 gagaccgtcg gcggcggcca gggggcgagc cacaggggcc ctggtccctc cggcgtacac
      301 atcgggatga gcaatactct caatactccc accgaggcct tcgagatgga gtacccgatg
      361 cgcgtcgagc actacgagct gctctacggc tcgggcggag aaggcgggca cctcggcggc
      421 gacggcatcg tccgctccgt gaaagtcctc gaaccagcga gcctctccct gctcaccgac
      481 cgcaggcgcc acgggccgcg cggcgtcgag ggcggagggc ccggcagggt cggccgtaac
      541 ctcttgaacg gcgaggaact gcctcccaaa gtgagcaggg aactcgccgc gggagacgtc
      601 gtgacggtcg agacgcctgg cggcggaggt tatgggaaac ccccgaattc tcagagccca
      661 tagggaagtc ccggcgaacc tcttagacga ctattgacgc gacgaacggg gtaagcacct
      721 ttgtatgggg atacaggctc gaccagaagc cgggtggcat gcccgatagg ccacatcagc
      781 acggaatgtc gcgtcggcgg ttcgtcaagg acgtggggct cggcgtagcc gggctgtcca
      841 tgccagggtt actcgcttgc cgcggcgctg tctcggccca gaccgtagga tccgacgccc
      901 tccaccgccg cgtacgcgcc gagctgagga agttcaccga ctggctcgat cagaacggcg
      961 tcgatggcta catcggggag gtcggatggc ccgacgatta cgcgggcgac gccgctttgt
     1021 ggaactcgct cgcgaatgcc tggtacggcg acgctgcggg actgtgggtc acacagtggt
     1081 ccacgggcga gtggtggggc acggattaca agctggcggc ctacgaggat cggcactcgc
     1141 ccaacggcgt tgactccgcc aacacccagg ctgtagtgac cgaggcgcat ccgacgacgc
     1201 cttcttactt gcgcggggtc aacgtctcag gcgcagagtt cggcgctccc ttctcgctcg
     1261 atgcgacgag caacttctcc aacaggaatc cggggcgcta cgacaccgcg taccactacg
     1321 acggacaggc gaccttcgat tatctggcga gccgtggcat ccggctcatc aggctgcaat
     1381 tccgctggga gcgcatccag cccacgccgg gaggcccgct tgacgggacg gaactacgca
     1441 ggctaaaagc ggcggtccgt cgcgccacag gcgctggtct cgaagtcatc ctcgacgtcc
     1501 acaacttcgg cgattactac atcgagcgca acgggcgcgg cgtccggttc ccgatcggct
     1561 ctgaccgcct gcccataagc cgcttcgcag acctctggcg ccgcctgagc aacaacttca
     1621 aagcgatgcc aggcatcgtc ggctacggcc tcatgaacga gcccgccgga atgcctcgcg
     1681 tcggggactt ctcgcccgcg aaggtgtggg agaaggcctc gcggcgggcg ctcgacgcca
     1741 tccgctccaa cggcgacctt agcctggtgc tcgtccaggg ctaccagtgg gccggagcgc
     1801 aagattggcc cacgaatcat ccggcgtcct ggatcgacga ccataacttc cgctacgagg
     1861 cgcaccacta ctgggatcgc aacaactccg gcgcctacag caacagctac gcggaggaag
     1921 tcgcagaggc ccgatctaga ggctactagc cgctccgaaa ggaacgagaa atgcagggtg
     1981 tggtcgaaag catctacgtt accggcgaag gaagcgcgtc gatggagcgc gtcgatgaag
     2041 tccgtactat cgaggggtgt ggcatcgagg gcgaccgcta ctgcgagggt atggggtact
     2101 ggacgaactt cggcgacgtt tgcgaagtca ccctgatctc ctccgaggac ctcgaacaca
     2161 ttgagcggga actcggcata ggcgtgggaa acggcgagca caggcgcaac atcgtcacac
     2221 gaggtgtcag cctcaaagtc ctgcgtggaa agcggttcag gatcggggat gcggtcctcg
     2281 agtacgacgg gccccgtcct ccttgcaggc acgtccagga cctgaccgag ccaggcatga
     2341 cgagggcgct caagggacgc ggtggtatct gcgctcgcgt tgttcaggct ggccgcatca
     2401 gggcgcgcga cgccatcgag atccagtaac tttcagccgc agagctcgcc ggggttctcg
     2461 cgcaggcacc cgcttccggg gacaggatca ttctggtaca gccagtagtc ggtccatacc
     2521 cggtccatca gggcatcctt gctcatgggc agcgctatga ccattggaag gaaaaacagc
     2581 gtcaacgcca cggcgcagac ggtgaagccc acgaccaccc agggtcccca ctgcgggcgg
     2641 ttccagatcc tgcagagcca gtaaacgagg gccaggatgg cgaagggata gatgggcaga
     2701 tagttgtaga tgtagggtat cctggtgcct ggaacccacg gcgccaggag gaaaaagtag
     2761 cctagtacta tggggatgat cgggtcgtcg gcagagaact tcctggcgat cgccttgcgc
     2821 gctatgtcga aggcgccggc caacacggcg agtgtgctgc tccaccagat cagggggttg
     2881 ccgatcgcga cgaccacctg caagagactc tccgcgttaa ccccgtagaa gaagcgtata
     2941 ggcctgagca tgatcggcca gctccaccat ggcgagcccc acggatggtc gacggcgacg
     3001 gtgatctttt gcgccgcgtg cacgtgccag ttccacgtcc acacccacgc ctctaccggg
     3061 ttcatcgtca cgcttatcag cgccccgacg tagatcacgg cgatgtagat gaccgcggag
     3121 atccagagcc ccgccagaaa cgggcggagc aagcccttgc gccagagcac gtagccggcg
     3181 ggtatggcca cggggaaagc ggcccacttc accgcgatag ccagacccag aaacacggag
     3241 gtccagatca cctgcctgcc cgtctcggcc cgcagcgcgg cgaggaaggt cgcgagcacg
     3301 aagaaaacga cgaagatgtc cataaccccg gtgcgggagt ggactatgaa catcgtctct
     3361 accgcgaaga acgtcagcat gagaatggcg gcgaccctct ccctcaggca gtaccagccg
     3421 aggactgcga ccagcggcag aagggccagg ccgaacaggg taggcatgat cctccagccg
     3481 agaggttcgt ccccgaggaa gaagatgccg acggccagga tgaactttcc gagcggtgga
     3541 tgcagatcga actggtagac gccatcgagg aagttgttgg ccattgcggg gaagtacttc
     3601 tcgtcccaca tgatcttggg cgggaagcca aggtgatata gcctggccca tactccgagc
     3661 gcgaccgcac agacggtgat gagcagcatt gagggatagg cccatgacct gtcgtggctt
     3721 aggaacgact tcataaggcg cacgttacta ggtttggcga cggtttgtaa agtaggagtg
     3781 cggcaaaagg tgattcgaga tcatgcacca aacctgggct cgcggtcacg caggatcatt
     3841 gcccttccca ttagtatggc gacgagcgcc aggagggcga taacgcccga gacgagccag
     3901 gccgtgccgt aggaagtcgc ctcgacgacg aggccgaaga cgagaggccc gagcgcggcg
     3961 cctccggagg cgccggtctg cgtgatccca gtggccgccg ccggcgcgcc ggggttggtc
     4021 ttgacgacgg cgaagttgaa gaggccgggc cacccccatc ccgccccgaa tgcgagcagg
     4081 gtggcaggca agatgaggcc
//
LOCUS       DATAIR010000703         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_257684, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000703 DATAIR010000000
VERSION     DATAIR010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257684"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..798
                     /locus_tag="VHM69_16630"
     CDS             163..798
                     /locus_tag="VHM69_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742069.1"
                     /translation="MFALTPGDLSGRILDCAAGPASFNAELTSDGHDVISCDPVYSLT
                     ADEIRSRIDATRGTVVGNARAAQNEFVWRDFASPEHLGEVRMAAMQRFLADFPEGLRE
                     GRYLVQALPHLDFHGDEFDLALCSHFLFTYSDQLSTDFHVSAIQEMCRVAGEARVFPL
                     LKSYGGPSPHLEPVVNALRKRGYEAEIREVPYEFQRGGDKMLAVRRSTSAG"
     gene            complement(804..1310)
                     /locus_tag="VHM69_16635"
     CDS             complement(804..1310)
                     /locus_tag="VHM69_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742070.1"
                     /translation="MTSNSKAEIRLFEMREAHFQTYREDLAREYAADKVRAGVWSQAE
                     AEGRARRELDELLPEGPDTPGHFIYSVRDELVPAEVGIVWFAVIDSGVGRSLWIYDLV
                     IHEQFRRRGYARRTLELVEEKAIELGARRVELHVFGHNHGARTLYEEVGYGVTSIIMA
                     KPVSAESG"
     gene            complement(1307..2236)
                     /gene="holA"
                     /locus_tag="VHM69_16640"
     CDS             complement(1307..2236)
                     /gene="holA"
                     /locus_tag="VHM69_16640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX2742071.1"
                     /translation="MAVYLLLGDDEERKARGVEKLREGRAVEGYDAAGTSPETIVSAC
                     NSFSLFGEGPFVVLKNLDAWNAAQKAVVVDYLRDPSPGADLILLGKKLGARERLLSAV
                     KKNGEVHNFEQPKGKALIRWVVGHAKKLGLDLPEDVAEDLTNRCSGDKMRLIQESEKL
                     ALYVGDGTATHDDVAALCPPDVQSNIFAFVDSLAAGEQDRALRLLEDLINTGEPPLRL
                     TFMIRRQFQLVARARALLQRGTPQKEVASLLKVPPFVARKLEEQGRKLDGEDLERAMN
                     LVLGLESGLKGGSNLSDELQVEMTVLKLSEVPR"
     gene            complement(2246..>2438)
                     /locus_tag="VHM69_16645"
     CDS             complement(2246..>2438)
                     /locus_tag="VHM69_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742072.1"
                     /translation="SSLPFASITTPGVTPLLVGLFYAGCVPPIVAERVFSSEHSPLWA
                     ALLLLWTMLWLVLVGAGSV"
BASE COUNT          477 a          797 c          692 g          472 t
ORIGIN      
        1 tacaaccagg ccgcgaccaa gggtgtcatt cacccgaaca gggccgcccg caaggtcagc
       61 cgcctcgaca aagcactcgc agccaaacaa ggcgaataag caacagttcc caggctggat
      121 gaggtagctc cctggggaag atcgaggggc gaatacgtca ggatgttcgc cctgacgccc
      181 ggcgacctct caggcaggat cctcgactgc gccgcgggac ccgcgagctt caacgccgaa
      241 ctcacttcag atggccacga tgtcatctcc tgcgatccgg tctacagcct caccgccgac
      301 gagattagat cccgcatcga cgctacccgt ggcaccgtcg tgggcaacgc gcgggcagcc
      361 caaaacgagt tcgtctggcg cgactttgca tcaccggagc atctgggcga ggtacggatg
      421 gcggccatgc agcgattcct tgcggacttc cctgaaggac tacgggaggg acgctatctg
      481 gtccaggcgc tcccccacct cgactttcac ggcgacgagt tcgacctcgc gctctgctcc
      541 cacttcctct ttacgtactc cgatcaactc tccaccgact tccacgtctc cgcgatacag
      601 gagatgtgcc gcgtcgcggg tgaagcgcgt gtctttccgc tgctcaagag ctacggaggg
      661 ccttcgccac acctggagcc ggtagtgaac gccttgcgaa agcgagggta cgaagcagag
      721 atcagagaag tcccatacga attccagcgt ggaggagata agatgctcgc ggtgagaaga
      781 tcaaccagcg caggctgagg acttcagccg ctttcggcac ttaccggctt ggccatgata
      841 atgctcgtta cgccgtagcc cacctcctcg tatagcgtcc gtgccccatg attgtgcccg
      901 aagacgtgca actccaccct cctcgcaccg agttctatgg ccttctcctc gaccagttca
      961 agagtacggc gcgcgtatcc tctgcgcctg aactgctcgt ggatgacgag gtcgtagatc
     1021 cagagcgaac gcccgacgcc ggagtcgatt acggcgaacc atacgatccc gacctcggca
     1081 ggaaccaact cgtcccgtac cgagtagatg aagtggcccg gcgtgtccgg tccttcaggc
     1141 aacagctcgt ctagctctcg ccttgctcta ccttccgcct cggcctgtga ccagacgcct
     1201 gcccgaacct tgtccgccgc gtactcgcgc gccaggtcct cacggtaggt ctgaaagtgc
     1261 gcttcgcgca tctcgaatag cctgatctcc gccttgctat tcgatgtcat cggggcactt
     1321 cggagagctt cagcacggtc atttcgacct gtagctcgtc ggaaagattg ctcccgcctt
     1381 tcagcccact ctccaggcca aggacgaggt tcatggcgcg ttcgaggtct tccccgtcca
     1441 atttgcggcc ctgttcctcc agctttcggg ctacgaaagg aggtactttg aggaggctcg
     1501 caacctcctt ctgtggggtg ccgcgttgca ggagggcgcg ggcgcgagct acgagctgga
     1561 actggcgacg gatcatgaac gtaagccgga gtggcggctc tcccgtgttg atcaggtctt
     1621 ccagaagtct caatgctcga tcctgctcac ctgcggccaa tgaatcgacg aacgcgaaga
     1681 tgttggattg gacgtctggt ggacagaggg cagccacatc gtcgtgggtc gcagtgccat
     1741 ccccgacgta gagcgcaagc ttctctgact cctgaatcag ccgcatcttg tcgccggagc
     1801 aacggttcgt caggtcctcc gcgacgtcct ctgggagatc caggcccagc ttcttcgcgt
     1861 ggccgactac ccatctgatg agggccttgc ccttcggttg ctcgaagtta tggacctctc
     1921 cattcttctt gaccgccgaa agcagcctct ctcttgcgcc gagcttcttg ccgaggagga
     1981 tcaggtcggc acccggcgaa gggtcccgca ggtaatccac caccacggcc ttctgggccg
     2041 cgttccaggc gtcgaggttc ttcaagacca cgaacggtcc ctccccaaag agcgagaacg
     2101 agttgcacgc cgagacaatc gtctccggac tcgtcccggc cgcatcgtat ccctcgaccg
     2161 cacggccttc acgcagcttc tctacgccgc gcgctttgcg ctcctcgtcg tctccaagca
     2221 acaggtaaac agccatggat gtattctaaa cgctaccagc accgacgagt accagccaaa
     2281 gcatcgtcca cagcaaaagg agtgctgccc acagcggact gtgttcactc gagaagactc
     2341 gctcggcgac tatcgggggg acgcatcctg cgtagaacag tcccacgagc agcggtgtca
     2401 ccccaggtgt cgttatggac gcgaacggga gcgaggag
//
LOCUS       DATAIR010000704         7407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_257808, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000704 DATAIR010000000
VERSION     DATAIR010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257808"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHM69_16650"
     CDS             complement(<1..407)
                     /locus_tag="VHM69_16650"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX2742073.1"
                     /translation="MTESARATAARELQDHIRTVPDFPRPGISFKDITPLIEDGRALH
                     MAVDALAEATDNISYDRILSAEARGFVFGTALAYRARKGLILARKPNKLPRETISATY
                     ELEYGTDALEAHADSIPEGTRILVADDLLATGGT"
     gene            complement(404..1144)
                     /locus_tag="VHM69_16655"
     CDS             complement(404..1144)
                     /locus_tag="VHM69_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2742074.1"
                     /translation="MNRVENGGINQENNVNADEEYHPTGERYSATKDSFTIGEVVERL
                     RPEFPTLSVSKIRYLERRRLINLTRTRGGYRLFSSQDIELLRYILTLQDKEYLPLKVI
                     KKRIEGGGAFTASDSAGDLSRVLEDRTYTREELADTLESDTRFVEELVNVGVIGRSGE
                     LTQRDAEIARMALEMAKYSIQPRHLRGIMAAVDREAALFKQVVNPELRSGDDGRVAEA
                     MNRARHLADVDSRMKQLMMANVIDTFVP"
     gene            complement(1174..1623)
                     /locus_tag="VHM69_16660"
     CDS             complement(1174..1623)
                     /locus_tag="VHM69_16660"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2742075.1"
                     /translation="MRLCPQCGAEVSAKMKFCAECGSKLDTGQSTVSFAPSFGEDAEA
                     ITSGGAEGAALIELDQVEGTAGRRMHDLSDDVVTVGRGQESNIFLDDVTVSRKHAEIV
                     HGDRGYRIRDVGSLNGTYVNRVRVDAVDLRSGDEIQVGKYRFRFVLS"
     gene            complement(1623..1868)
                     /locus_tag="VHM69_16665"
     CDS             complement(1623..1868)
                     /locus_tag="VHM69_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742076.1"
                     /translation="MPRGGGFEWEEAEDITALSEADLRVRLEALCDEERAISYRRRVF
                     QGRIDLIRAELVERGAVAVSPEDLARVLLGGSPSREL"
     gene            complement(1922..4426)
                     /locus_tag="VHM69_16670"
     CDS             complement(1922..4426)
                     /locus_tag="VHM69_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2742077.1"
                     /translation="MKAVIMAGGQGSRLRPLTSDQPKPMIPIVNVPCMEHIVGLLKRH
                     GITDVAVTLQFMPDEIRDYFGDGSDWGVNMRYSIEDAPAGTAGSVKMAEQQLGLEGER
                     LLIISGDALTDADLSHLIEFHEEKGSEATMVLKGVENPLDFGIVITEEDGRISRFLEK
                     PAWGQVFSDTVNTGIYLLEASVMDEIPDPEEGEYDFSKELFPKLLDDGRPLYGYVTED
                     YWEDIGTLEQFASAQRDVLDGKVKGVRPPGTRLRENIYVGRRAQVDDDDLQGPVVIGE
                     NVRVDEGARISPYSVIADNVVVSAGAKVERSVIAEGTYIGEGAEIVDALVGRNSYVQA
                     RARILERSALGDDVIVGEGATIAPEVKVYPHKTIESGANVTQSLIYETMGLRTVFKRG
                     IVSGKFNVDLTPEFVTRLGSSFGTTLDPGAVVTLGRDSSLSAQVAKRAMTAALLGTGI
                     NIRDLRAAHAGVVRHDVLAGKSSAGVHVRAGGDPDEVEILYFSADATPMTEGDQRAVE
                     KAYVREEYRRAHGGDIGELIYPGRVVEQFVERLERAVQREMTSGATIVVDFCGGVAGL
                     VASRVFSRLGVSAVVMEGFANANAVGEAGRMNLEESIERVARIVPTVDAAFGAVVSPE
                     AEYVQFIDEKGEWVPPDVLLACLIDRMRPQKAVLPINLSRRYEELVEEGGGSVEMSRT
                     GLGNVAIKAAGVRADVASPGDGRYIFPAFLPAPDCFMTLASALSVFRETALSEVREKF
                     GDSVGNVVRERIECPWSAKGRVMRGLAEKFGGDPDAILTEGVLLSVEDGWVLMLPDPD
                     SPVFYVYAESERKDHIMEEYVDLVKSLIDDQAMLSS"
     gene            complement(4622..5923)
                     /gene="der"
                     /locus_tag="VHM69_16675"
     CDS             complement(4622..5923)
                     /gene="der"
                     /locus_tag="VHM69_16675"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX2742078.1"
                     /translation="MSPLPVVAVVGAPNVGKSSLVNRIMRRRNAVVSEQPGTTRDRAY
                     NRAEWAGREFTLVDTGGMEPYAKVGLQALVTLQARVAVEEADVVLHLTDARLGPTEAD
                     AAIARELRRKGKTVLAVNKLDNPADDTERHRFYSLGEGEPHAISAIHGLGVGDLLDVV
                     VSLLPEHEPESEDQLPKLAIVGRPNVGKSTLLNRLLGTDRAVVSEKAGTTTDAVAGEV
                     EVEVDGEVERYLLLDTAGVSKSARRASGVPYYSALRTSEAIRRSDVALLLVDAVEGIV
                     AGDLRIAREIEEAGSSCGVLTNKRDLVPAERLREIEGLISARLTDLKPRAIAISALEG
                     AGVDGVLPFAARIGRAYRMRVSTHELAEFINDVVGRSAPPRGVKIRYATQTGTAPPRF
                     TLFANRPKDLPESYLRYLENALRQRYGLWGVSVRIRVKSSR"
     gene            complement(5926..6579)
                     /locus_tag="VHM69_16680"
     CDS             complement(5926..6579)
                     /locus_tag="VHM69_16680"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2742079.1"
                     /translation="MATVAPFKGKSTPGRMSPRYHLFRRAMMGLCRILFGLTIHGAER
                     VPSEGPLIVASNHRHYADPVLVCVAVPRRLQWMAKKQLFVFPFRRFFEFIGSFPVDRE
                     GGGRGAIRAALAFLAEGWALGIFPEGTHRGAGAAREAKSGVVVLAVRSGAPVLPVYVG
                     KLPGPLARLRGRRLHIFVGEPVEIDRDLRGGVAYRTAADGIMRAIYALPKQHGSEAG"
     gene            complement(6579..7244)
                     /gene="cmk"
                     /locus_tag="VHM69_16685"
     CDS             complement(6579..7244)
                     /gene="cmk"
                     /locus_tag="VHM69_16685"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2742080.1"
                     /translation="MGGILVAIDGPAGSGKSSVARAVAGRLGVVNLNTGAAYRAVALL
                     ALREDVDLADGSGLATLAERVDLDPEGASIDGERVAETDLRTPEVSAAASRVSAHAQL
                     REVLLPVQRMAAEKARERGGAVVEGRDIGTVVLPDAELKVYLSAAAEERARRRASQTG
                     REGELDRIRAAIARRDRQDSEREVSPLKPAADAVVLDTTSLSLEEVTSEVLDLAQNLR
                     RGA"
BASE COUNT         1249 a         2499 c         2219 g         1440 t
ORIGIN      
        1 gttccgccgg tggcgagcag gtcgtctgcg acgaggatgc gggtaccttc cgggatcgag
       61 tctgcgtggg cctccagggc gtccgtgccg tactcgagtt cgtaggtagc ggagatggtc
      121 tccctcggaa gcttgttcgg cttgcgggcg aggatcaaac ccttcctggc gcgataggcg
      181 agggcggtgc cgaagacgaa gccgcgggct tcggctgaga gtatgcggtc gtaggagata
      241 ttgtctgtgg cttcggcgag ggcgtccacg gccatatgca gggctcgccc gtcctcgata
      301 agcggggtta tgtccttgaa ggagatcccc ggccgcggga agtcgggcac tgtgcggatg
      361 tggtcctgga gctcccttgc ggcggtcgcg cgcgcggatt cggtcacggg acgaaagtat
      421 ctatcacgtt cgccatcatc agctgcttca tgcgcgagtc cacgtcggcg aggtgcctgg
      481 ctctattcat cgcctcggcc acacgtccgt cgtcgccgct gcggagctcg gggttcacta
      541 cctgcttgaa gagggcggct tcgcggtcca cggcggccat gatgccgcgg agatggcggg
      601 gttggatcga gtacttcgcc atctcaagtg ccatgcgcgc tatttcggcg tcccgctgcg
      661 tcagttcacc gctacgtcct ataaccccca cgttgacaag ctcctcgacg aagcgagtgt
      721 ccgattcgag ggtgtcagcc aactcctcgc gggtgtacgt cctgtcttcc aggacccgcg
      781 agaggtcacc cgccgagtcg ctggcggtga atgcgccccc tccttcaata cgcttcttga
      841 tgaccttcag cggaaggtat tccttatcct gcagcgtcag gatgtacctg aggagctcga
      901 tgtcctggga cgagaacagc ctatacccac cacgcgtgcg ggtcaggttg atcagacgcc
      961 tccgctcgag gtagcggatc ttgctgacgg agagggtcgg gaactcgggc ctgagtcgct
     1021 cgacgacctc gccgatggtg aagctgtcct ttgtggcgct gtacctctcg ccggtgggat
     1081 ggtattcctc gtctgcattg acgttgttct cctgattgat gccgccgttc tccaccctgt
     1141 tcactcccat cctctcgaat ccagtgtccg ctctcaggag agcacgaacc tgaaccggta
     1201 tttccctacc tgtatctcat ccccgctgcg caggtcgacc gcgtcaaccc tgacgcggtt
     1261 gacgtaggtc ccgttgaggc tcccgacgtc acgtatcctg tacccacggt caccgtgcac
     1321 gatctcggcg tgcttccttg agacggtgac gtcgtcgagg aagatgttgc tctcctggcc
     1381 acggccgaca gtgacgacat cgtcactgag gtcgtgcatc ctgcgtccgg ccgtgccctc
     1441 cacctggtcc agctcgatca aagccgcgcc ttccgcgcct ccggatgtga tagcctccgc
     1501 atcctctccg aaactcggcg cgaaagaaac ggtgctctgg ccggtgtcga gcttggaccc
     1561 gcactcagca cagaacttca tcttcgcgga gacttcggct ccacactggg gacacagcct
     1621 catcaaagct ccctactcgg ggaaccgccg agcaaaaccc gcgcgagatc ctcaggcgag
     1681 actgcgaccg cgccccgctc cacgagctcg gcacggatca gatctatcct cccctgaaat
     1741 acccgccgtc tgtaactgat ggcgcgctcc tcatcacaca gcgcttcgag ccttactctc
     1801 aggtccgctt cagagagagc tgtgatgtcc tcagcttcct cccactcgaa tcccccaccc
     1861 cgaggcaacc ctgaatcatc catggttgaa gtataacctg ccctgaacct gaagtgcaat
     1921 ctcagcttga gagcatcgcc tgatcgtcga taagagactt caccaggtcg acgtactcct
     1981 ccatgatgtg gtctttcctc tcggattcgg cgtagacgta gaacacgggg ctgtcgggat
     2041 ccgggagcat taggacccag ccgtcctcga cgcttaggag cacgccctcg gtcaggatag
     2101 catccgggtc cccgccgaat ttctcggcga ggccgcgcat tacgcggcct ttggccgacc
     2161 acgggcactc tatgcgctcg cgcacgacgt ttccgaccga atccccgaac ttctcgcgca
     2221 cctccgagag tgccgtctcg cggaaaactg agagtgcgct cgcaagcgtc atgaagcaat
     2281 caggagcggg gaggaaagct gggaagatat agcgtccgtc gcccggtgaa gcgacgtcgg
     2341 cacggacgcc cgcggccttt atggcgacgt tacccaggcc cgtacggctc atctcgacgg
     2401 agccgccgcc ttcctcgacc aactcctcgt atctcctgct caggttgatc gggagcacgg
     2461 ccttttgcgg gcgcatacgg tcgatgaggc aggcgagcaa gacgtctggg gggacccact
     2521 cacctttttc gtcgatgaac tgcacgtatt ccgcctcggg gctgacaacc gcgccgaaag
     2581 ccgcgtccac cgtcggcacg atgcgcgcga cgcgctcgat gctctcctcc aggttcatcc
     2641 ttcctgcttc accgacggcg ttggcgttgg cgaagccctc catgaccacg gcgctcacac
     2701 cgagccggga gaacactcgg ctcgccacca gtccggcgac tccgccgcag aagtccacga
     2761 cgatcgtcgc gcccgaggtc atctccctct gcacggcgcg ctccagacgt tccacgaact
     2821 gctccacgac gcggccgggg tagatcaact ccccaatgtc cccgccgtgg gccctgcggt
     2881 actcctcgcg cacgtacgcc ttctcgacgg ccctctggtc cccctcagtc atcggagtcg
     2941 cgtcggctga gaagtagagg atctcgacct cgtcggggtc gcctccggcg cgtacgtgga
     3001 ctcccgcgga agacttgccg gcgaggacgt cgtgcctcac gacgccggcg tgcgcggccc
     3061 tcaggtccct gatattgata cctgttccga gcagcgcggc cgtcatcgcc cgcttggcga
     3121 cctgggcgga gagcgaagag tctctgccga gcgtgacgac ggcgcctgga tcgagcgtgg
     3181 tcccgaagga ggaaccgagg cgggtgacga actcgggggt gaggtcgacg ttgaacttgc
     3241 cgctgacgat gccgcgcttg aacacggtgc gcaaccccat ggtctcgtag atcagggact
     3301 gcgtgacgtt ggccccgctc tcgatggtct tgtgcgggta gacttttacc tcgggggcga
     3361 tggtagcgcc ctcgccgact attacgtcgt ccccgagggc gctgcgctcc aggatgcggg
     3421 cccgcgcctg cacgtaagag ttacggccga cgagcgcgtc tacgatctcg gcgccttcac
     3481 ctatgtaggt gccctccgcg attacgctgc gctccacttt cgcgcctgcg gagacgacga
     3541 cgttgtccgc gattacggag taggggctga tcctggctcc ttcgtcgacg cgcacgttct
     3601 caccgatgac cacgggtccc tggagatcgt cgtcgtcgac ttgcgccctg cgccccacgt
     3661 agatgttctc cctgaggcgc gttccaggcg gcctgactcc cttgaccttg ccgtcgagga
     3721 cgtcgcgctg tgcgctcgcg aactgctcca gggtacctat atcctcccag taatcctcgg
     3781 tgacgtatcc gtagagcggg cgtccgtcgt cgagtagctt gggaaatagc tccttcgaga
     3841 agtcgtactc gccctcttcg gggtcgggga tctcatccat cacagaagct tcgagcagat
     3901 agatgcccgt gttcacggtg tcggagaaga cctgtcccca tgccggcttc tcgaggaagc
     3961 gcgagatacg cccgtcctct tcagttatga cgatgccgaa atccagaggg ttctcgacgc
     4021 ccttcaggac catagtggcc tcggagccct tttcttcgtg gaactcgatg agatggctca
     4081 ggtcggcgtc ggtcagggcg tcgccgctta tgatgagcag acgctcgcct tcgagcccga
     4141 gttgctgctc ggccatcttc acggagcctg cggtgccggc gggcgcgtcc tcgatcgagt
     4201 agcgcatgtt cacgccccag tcagagccgt cgccgaagta gtccctgatc tcatccggca
     4261 tgaactgcaa cgttacggcg acgtccgtga tgccgtggcg cttgagcagc cctacgatgt
     4321 gctccataca gggtacgttg actatgggga tcatcggctt gggctggtcg cttgtgagcg
     4381 gccgcaggcg gctgccctgc ccgccggcca tgatgacagc cttcatcgga cctcacccaa
     4441 gacgtgccgc gcacgcctta cacgtgtgaa cctcgcctct gcgtcgaaca tgctctctcc
     4501 tgatccttcc cgtttttcac ggcgcaccgg cgtcaccgtg agcgaggcat cggcctcgcg
     4561 ttcccagcaa gagtttatct tacaagcgaa gcgagtttgg gaaacccgac aaaggaactt
     4621 gctacctact gctcttgacg cgtatcctga cgctgactcc ccagagtccg tatcgctgcc
     4681 gcagcgcgtt ctccagatat cgcaagtagc tctcaggcag gtctttcggc ctgttggcga
     4741 agagggtgaa cctcggcggt gccgtgcctg tctgggtggc gtagcggatc ttgactcccc
     4801 tcggcggcgc gctgcgtcct accacgtcgt ttatgaactc ggcgagctcg tgggtagaga
     4861 cccgcatcct gtaggcccgt cctatgcgcg ccgcgaatgg caacacccca tcgaccccag
     4921 cgccctcgag cgcggagatg gctatggccc tgggcttcag gtcggtgagg cgcgccgaga
     4981 tgagaccctc gatctcccgc agccgttctg ccggcacgag gtcgcgcttg tttgtcaaga
     5041 ccccacatga agatccagcc tcctctattt cacgggcgat cctgaggtcc ccggccacga
     5101 tgccttcgac agcatcgacc aggagcagcg cgacgtcgga acgacggatg gcctcggagg
     5161 tccgcaaagc ggagtagtaa ggtacgccac tcgcccgcct cgcgcttttg cttactcccg
     5221 ccgtatccag caggagatat cgctccactt ctccatctac ctctacttca acttcacctg
     5281 ctacggcgtc agtggttgtc ccggccttct ccgagacgac tgcccgatcg gtgccgagca
     5341 gacgattgag aagcgtgctc ttgccgacgt tcggcctgcc gacgatggcg agcttcggca
     5401 gctggtcctc agactccggc tcgtgttccg gcagaagtga gaccactacg tcgagaaggt
     5461 ccccgacgcc gaggccgtga atagctgaga tcgcatgtgg ttctccctca cccagggagt
     5521 aaaaccgatg tcgttcggta tcgtcggcag ggttatccag cttgttgacc gcgagtacgg
     5581 tcttgccctt acgtctgagc tcgcgggcta tggcagcgtc ggcctcggtc gggccgagtc
     5641 tggcgtcggt gagatgcaga accacgtccg cctcctcgac agccaccctc gcctgcagcg
     5701 tcacgagggc ctggagaccg accttcgcgt aaggctccat cccgcccgta tcaaccagcg
     5761 tgaactccct gccggcccac tctgcccggt tgtacgccct gtcgcgcgtg gtgccaggct
     5821 gctccgagac caccgcattg cgacggcgca tgatccggtt taccagcgag ctcttgccca
     5881 cgttcggagc cccgacgacc gcgaccacag ggagagggct caagttcagc cagcctcgct
     5941 gccgtgttgc ttgggaaggg cgtagatggc gcgcatgatc ccatccgcag ccgttcggta
     6001 ggcgacaccc ccgcgcagat ctctgtctat ttccaccggc tcgcccacga agatgtgaag
     6061 cctccggcca cgcaggcggg cgagaggtcc cggcagcttc ccgacgtaga cgggcagaac
     6121 gggggcgcca ctgcggacag cgagtaccac gaccccgctc tttgcctccc gcgctgctcc
     6181 tgcgcccctg tgggtacctt cagggaagat acccagcgcc cacccttcgg ccaggaaagc
     6241 gagagcggcc cgtatcgctc cacgtccccc accctcccgg tcgacgggga atgaacctat
     6301 gaactcgaag aacctccgga acgggaagac aaagagctgc ttcttcgcca tccattgcag
     6361 acgccgaggt acagccacgc agaccagaac ggggtcagcg tagtgcctgt ggttggaggc
     6421 cactatcaag ggtccctccg aaggaaccct ttccgcgccg tgaatggtca gtccgaacag
     6481 gatcctgcac agtcccatca tagcccgccg gaagagatgg tatctggggg acatccggcc
     6541 tggcgtactt ttgcccttga agggcgcgac ggtggccatc acgcgcccct gcgtaggttc
     6601 tgggcgaggt ccagtacctc ggaggtaact tcttcgaggc tcagtgaggt cgtgtcgagg
     6661 acgacggcgt cagcggccgg cttgagaggc gagacctccc gctcggagtc ctggcggtcg
     6721 cgcctggcta tagccgcccg gatgcgatcc aactccccct ccctgcctgt ctgggacgcg
     6781 cgcctgcgcg ccctctcttc cgcggcggcg gatagataaa ctttcagctc agcgtcaggt
     6841 aagacgacgg taccgatgtc cctgccctcg acgacggctc caccccgctc ccgcgccttc
     6901 tccgccgcca tccgctgcac ggggagcaag acctccctga gctgcgcatg cgcagagacc
     6961 ctggaggccg ctgccgacac ctcgggcgtg cgtagatccg tctccgccac ccgctccccg
     7021 tcgatgctcg cgccctcggg gtcgagatcc acgcgttctg cgagcgtcgc cagcccggac
     7081 ccgtccgcga ggtcaacgtc ctcgcgtagc gcgagcaacg caacagcacg gtacgcagcg
     7141 cccgtgttga ggttcacgac cccgaggcgc ccggcaaccg cccgcgcgac cgaactcttc
     7201 ccgctcccgg cgggtccgtc tatggctacg agaattcctc ccatcggcca cggaagtata
     7261 tccgaaagcc cgcgctcttc accgtaagga ttccagccgc tcgaagtaat cgggaaaggt
     7321 cttggtcaca cacccggggt ccctgatcct tattcctccc cccaccagcc ccgttaccgc
     7381 gaacgccatc gccatcctgt ggtcgcc
//
LOCUS       DATAIR010000705         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_162774, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000705 DATAIR010000000
VERSION     DATAIR010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162774"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..650)
                     /gene="gcvH"
                     /locus_tag="VHM69_16690"
     CDS             complement(273..650)
                     /gene="gcvH"
                     /locus_tag="VHM69_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX2742081.1"
                     /translation="MSVPEDLQYTKSHEWVRTEDDTATIGITDHAQEELGDVVFVELP
                     SEGDTFEAGEAFGSVESVKAVSDLYAPVGGEVVEVNSALEDAPEKINEDPYGEGWIVK
                     LRTSDEADLLSPEEYERVVEEES"
     gene            complement(663..1742)
                     /gene="gcvT"
                     /locus_tag="VHM69_16695"
     CDS             complement(663..1742)
                     /gene="gcvT"
                     /locus_tag="VHM69_16695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX2742082.1"
                     /translation="MADLKHTPLYDEHKDLGARLVDFAGYEMPVQYEGIKAEHNAVRN
                     HAGIFDVSHMGEAVFRGPDAESAVQRLVTRDVSRLDVGQAGYSVVCYEDGGTVDDVIV
                     YRREGDFLVVVNASNREKDLAHFRENTEDLDVEVADESDDWALLALQGPEAEQLLQGS
                     TDTELSDLKPFRFVEGEVAGFAALISRTGYTGEDGFELYVSPDDAPGIWRTLIEAGAT
                     PAGLGARDTLRLEAGLCLYGNELDPGTTPLEAGIGFAVHLDKEQEFIGQTALKDEKEN
                     GLRKKLVGFEMEGRGIARHDHQVTVDDETVGHVTSGTRSPTLDRAIGLALVDPGVEDR
                     FDVVIRDKPVPAKTVPIPFYKRDKG"
     gene            complement(1745..3160)
                     /gene="gcvPB"
                     /locus_tag="VHM69_16700"
     CDS             complement(1745..3160)
                     /gene="gcvPB"
                     /locus_tag="VHM69_16700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX2742083.1"
                     /translation="MPFNLIRDKGREGRRGYTLPKPDVEMKKLEKILPKTALRKEKPG
                     LAEVDEPTVIRHYTNLSRENEGIDTTFYPLGSCTMKHNPRANEAAANVPGFAGLHPLQ
                     PEAQSQGALRIMHELQGFLSEVSGLPYVSLQPLAGAQGELTSVLMIKEFFEDSGDDGR
                     TTVLVPSTAHGTNPATASMAGFKSKEIGLDQHGCVDVDELREMVDETTAALMITNPNT
                     CGVYERNMAEIADVLHEAGAFLYMDGANMNANMGRMRPAEASVDIMHFNLHKTFSTPH
                     GGGGPGCGAIACSDDLAPFLPDPTVEKEGDEYRFVRPDKSIGRVATFHGNFGQMLRAW
                     NYIRMHGPDLKDVTDMAVLNANYVRAQLGDTYRIPYDELCKHEVVVQPPQGKKAIDIA
                     KGLIDHGYHPPTIYFPLVVKEAMLIEPTETESKETLDDFIGAMLELAETSQEDLEEAP
                     THAPTRRLDETRAARQLKARW"
     gene            complement(3150..>4410)
                     /gene="gcvPA"
                     /locus_tag="VHM69_16705"
     CDS             complement(3150..>4410)
                     /gene="gcvPA"
                     /locus_tag="VHM69_16705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX2742084.1"
                     /translation="EMLEAIGVEALDDLFADVSPKYEGELDLPPALSEYEALREVDAL
                     AKLNVSGLPIFLGAGAYDRIVPSAIGAIISRGEFLTCYTPYQPEISQGTLQSIFEFQS
                     MISELTGLEISNASVYDGANAVVEAALMTARLTKRDPKVAVSAGLNPRYREVIETYLV
                     ETVDLPFENGTTDFSELPDDVSGVFVQSPNFFGVVEDIEAASEAAHGVEALCVAVCDP
                     ISLSVLEAPGNLGADVAVGEAQPLGMPLTFGGPYAGYMATKEEFVRQLPGRITGETID
                     EDGKLAYVLTLRGREQDIRRAKANSNICTNQALTALAATVYTALMGPEGLREVAELSI
                     SKAHYLAERLQASGFRLRYPDAPFLWEFAVEFGEAGDVAKVNEALLEAGIVGGLDLGD
                     GAMLVAVTEKRTREELDAFVEVVANAL"
BASE COUNT          708 a         1376 c         1404 g          922 t
ORIGIN      
        1 gctctttaat tgttgccggt ttagtttcgc accaaccgac tgacaggatg atacatcgct
       61 cgttacactt tgtaaagggt ttggaacagc gaatttagaa tctttttgaa gatatctgca
      121 aaatgcacat aaatgttgcg gtggggcgtg ccagccggtc tcgacgacca cgagggttag
      181 tccgcagagg tacttgaccg gcgggatccg cgctggtgat cttgggtgag cagaggctcg
      241 tcaggaaccg ccgaggaagg cggggaggcc tcttatgact cctcttccac cactctctcg
      301 tactcttcgg gagagagcag gtcggcttcg tccgaggtgc ggagtttaac gatccagcct
      361 tctccgtagg gatcttcgtt gatcttctcg ggcgcgtcct cgagggccga gttgacctcg
      421 acgacctcgc cgccgacggg ggcgtacagg tccgagacgg ccttgacgct ctcgacgcta
      481 ccgaaggctt cgccggcctc gaaggtgtct ccctcggatg gcagctcgac gaagacaacg
      541 tcgccgagct cctcctgggc gtggtccgtg atgcctatgg tagcggtgtc gtcctcggtg
      601 cggacccact cgtggctctt cgtgtactga aggtcttccg gtacgctcaa agctatcctc
      661 ccctatcctt tgtcgcgctt gtagaacggt atcggcacgg tcttcgcggg cacgggcttg
      721 tcccttatca ccacatcgaa cctatcttcc actccgggat cgacgagcgc aagtcctatc
      781 gccctgtcga gggtcgggga cctggtgccg ctggtcacgt gtccgaccgt ctcatcgtcc
      841 accgtgacct gatggtcgtg gcgcgcgatc ccacgcccct ccatctcgaa gccgacgagc
      901 tttttgcgta gtccattctc tttctcgtcc tttagcgcgg tctggccgat gaactcctgt
      961 tccttgtcca ggtggacggc gaagccgata ccagcctcca gcggcgtagt cccagggtcg
     1021 agctcgttgc cgtagaggca cagtcccgct tcgaggcgca aagtgtccct ggccccgagt
     1081 ccggccgggg tggcgccggc ctcgatcagc gtgcgccaga taccgggcgc gtcgtccgga
     1141 ctgacgtaca gctcgaagcc gtcctcgccc gtgtatccgg tgcgtgagat caaggccgcg
     1201 aagccggcga cctctccctc gacgaaccgg aacggcttga ggtcagacag ttctgtgtcc
     1261 gtggaaccct gcaaaagttg ctccgcctcc ggtccttgca gggcgagaag cgcccaatcg
     1321 tcggactcgt cggccacctc cacgtcgagg tcttctgtgt tctcccgaaa gtgcgcgagg
     1381 tccttttcgc ggttcgaggc gttcacgacg acgaggaagt cgccttcgcg cctgtagacg
     1441 atcacatcgt ccaccgtccc cccgtcctcg taacagacca ccgagtagcc tgcctgccca
     1501 acgtccagac gtgatacgtc gcgggtcacg agacgctgga cggcgctctc ggcgtcaggg
     1561 ccgcggaaga cggcctcgcc catgtgagag acgtcgaaga tgccggcgtg attgcgtacc
     1621 gcattgtgct cggctttgat cccctcgtac tgaacgggca tctcgtagcc cgcgaagtcg
     1681 acgaggcgcg ccccgaggtc cttgtgctcg tcgtagagag gagtgtgctt gaggtccgcc
     1741 accactacca cctggccttg agttgacggg ctgcgcgggt ctcgtcgagg cggcgggtgg
     1801 gggcgtgcgt cggggcctct tcgaggtctt cctggctcgt ctctgcaagc tcaagcatag
     1861 cgccgataaa gtcgtcgagg gtctctttcg actccgtctc tgtcggctct atcagcatcg
     1921 cctccttgac tacgagtggg aagtagatcg ttggcgggtg gtaaccgtgg tcgatgagcc
     1981 ccttggcgat gtcgatggcc ttcttcccct gtgggggttg gacgacgacc tcgtgcttgc
     2041 acagctcgtc gtaggggatg cggtaggtgt ccccgagctg cgcccgcacg tagttcgcgt
     2101 tcaggacggc catgtcagtc acgtccttca ggtcggggcc gtgcatcctg atgtagttcc
     2161 aggccctgag catctggccg aaattgccgt ggaaggtggc cacgcggccg atactcttgt
     2221 cggggcggac gaagcggtac tcgtcgccct ccttctccac cgtagggtcg gggaggaaag
     2281 gcgcgagatc gtcggagcag gcgatggctc cgcatcccgg tcctccgccg ccgtgtgggg
     2341 tcgagaaagt cttgtgcaga ttgaagtgca tgatgtccac gctggcctcg gcggggcgca
     2401 tgcggcccat gttcgcgttc atgttcgcgc cgtccatgta gaggaaggcg cctgcttcgt
     2461 gcagcacgtc ggcgatctcg gccatgttgc gttcgtagac gccgcaggtg ttggggttgg
     2521 tgatcattag cgcggcggtc gtctcgtcca ccatctcacg tagctcgtcc acgtcaacgc
     2581 aaccgtgctg gtcgagcccg atctccttgg acttgaagcc cgccatcgag gcggtcgccg
     2641 ggttggtccc gtgcgccgtc gaaggcacga gtaccgtggt ccgcccgtcg tcaccggaat
     2701 cctcgaagaa ctccttgatc atcaggacgc tcgtgagctc cccttgcgcc ccggcgagcg
     2761 gctgcaggga gacgtacggt agtccggaga cttcagagag gaaaccctgt aactcgtgca
     2821 tgatgcggag cgcgccctgt gattgtgcct ccggctgtag tggatggagt ccggcgaagc
     2881 ctgggacgtt cgcggcggcc tcgttggcgc gtgggttgtg cttcatcgtg caggagccga
     2941 gtgggtagaa cgtcgtgtcg atgccctcgt tctcgcgcga gaggttggtg tagtggcgga
     3001 tgacggtcgg ctcgtccacc tcggcgaggc ccggcttctc cttgcgcagc gcagtcttcg
     3061 gcagtatctt ctccagcttc ttcatctcca cgtccggctt cgggagggtg tagcccctgc
     3121 gtccctcgcg gcctttgtcc cggatcaggt taaagggcat tcgcaaccac ctccacgaag
     3181 gcgtcgagtt cttccctggt gcgcttctcc gtaaccgcta cgagcatcgc cccgtccccg
     3241 aggtcgagtc cgcccacgat cccggcctcc agcaaagcct cgttcacctt cgccacgtcc
     3301 cctgcttcgc cgaactcgac ggcgaactcc cacaggaacg gcgcatcagg ataccgaagc
     3361 ctgaaacctg aagcctgaag cctctccgcc aggtagtggg ctttcgagat ggagagctcg
     3421 gccacctcgc gcagtccttc agggcccatg agggccgtgt agacggtcgc ggcaagggca
     3481 gtgagtgcct ggttggtgca gatgttggag ttcgccttcg cacgccttat gtcctgctcg
     3541 cggcccctca gggtgaggac gtaggcgagc ttgccgtcct cgtctatcgt ctctcccgtg
     3601 atacgtcctg gaagctggcg gacgaactcc tccttcgtcg ccatgtagcc ggcgtacggg
     3661 ccgccgaagg tgagcggcat cccgagcggc tgggcctctc ccacggccac gtcggcaccc
     3721 aggtttcccg gagcttcgag gacggagaga gagatcggat cgcacaccgc gacacagaga
     3781 gcttccacgc cgtgcgcagc ctcagatgcg gcctcgatgt cctcgacgac tccgaagaag
     3841 ttggggctct gcacgaagac gcccgagacg tcgtcaggga gttccgagaa gtccgtggtc
     3901 ccattttcga atggcaggtc aaccgtttcg acgaggtagg tctcgatgac ctcgcggtag
     3961 cgggggttca ggccggcgga aacagcgact ttggggtccc tcttcgtcag cctcgccgtc
     4021 atgagggcgg cttccacgac ggcgttcgcg ccgtcgtaca cggaggcgtt ggagatctcc
     4081 agtcccgtca gctcggagat catcgactgg aactcgaaga tggactggag ggtaccctgg
     4141 ctgatctcag gctggtacgg cgtgtagcag gtcaggaact cgccgcggga gatgatcgcg
     4201 cctatggccg agggtacgat cctgtcgtaa gcccctgcgc cgaggaagat cggcagcccc
     4261 gagacgttca gtttcgcgag ggcatctact tctcttagcg cctcatactc ggagagggcg
     4321 ggcggaaggt ccagttctcc ttcgtacttc ggactgacgt cggcgaagag gtcatccagg
     4381 gcttcgaccc cgatggcctc cagcatctcc
//
LOCUS       DATAIR010000706         7710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_349910, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000706 DATAIR010000000
VERSION     DATAIR010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349910"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VHM69_16710"
     CDS             complement(<1..992)
                     /locus_tag="VHM69_16710"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HEX2742085.1"
                     /translation="MSRLRSLDALVLLSSAAAVIVLLPLREVSGAFPPVAFSAALVLF
                     TAPGVLISHWFLRDDIYGVALVPVGFGISTGVFGLLGVPALILHLSIGEYLLAAGALL
                     AASLAGATWRTLRMKAPAVEDDPYGQRAGWLWAPFALLGGVLAFVGIRRVPNNYDDIW
                     VYLSWVKDFANAERLALRDPYFGEKLAELSRAKVNGWLLEQAALSRVSGLDTIELVLR
                     YLTPTLVIVALLMVYALARTLLKNERAAVLTASVYALFHIIFIQPSVQNVGVELAARI
                     SEDKYAARFLILPATLLFAVLFIESRKWRYLGLFTFFCWVAIAVHPSVLPAIGL"
     gene            1141..2118
                     /locus_tag="VHM69_16715"
     CDS             1141..2118
                     /locus_tag="VHM69_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2742086.1"
                     /translation="MEIFENLSASSRLESNELDAYSRAVRDVTERLEPAVISVGLPDG
                     RGGGSGVILGFDGISATAITNSHVVRGLRSRRAGNPGGDIQVTLSDGTSVHADVLGDD
                     PPSDLAVVRFETEEEPTVATLGDAGDLVVGQLVVAIGNPLGFQRSVTAGVVSALGRSI
                     RGESGRLIENVIQTDAAINPGNSGGPLADASARVVGINTAIIGGAQGIGFAVPVSGTF
                     RRVVFTLVTEGRVRRAYLGVVVESRQPRGEFKGGAGVRNIAPNSPAERAGVKPGDVIV
                     GLKGEPVHSNDDLLNLLDASSIGKDATLRVLRGNRELSLTVRPREQPAE"
     gene            2314..2544
                     /locus_tag="VHM69_16720"
     CDS             2314..2544
                     /locus_tag="VHM69_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX2742087.1"
                     /translation="MPDVTFMVNDIAEDSEKLERALTRLDFVDLVNVDSERGLVAVSY
                     EGGEAEIKRIEGAIEEAGHDAEPSPGADPDVD"
     gene            2548..2967
                     /locus_tag="VHM69_16725"
     CDS             2548..2967
                     /locus_tag="VHM69_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742088.1"
                     /translation="MTTISVAEAARFLDVPEEEARKLADDEGNVPLEVVRSRAGAAAI
                     ARDLAAAREEVGRLSGQLKEVRASNVRLQKEIKESVEERRRLTQEVLELRAAAEERLM
                     LMERIEHIARVEQDLEESTSELERLRNRRLLGRLLNR"
     gene            complement(2979..3668)
                     /locus_tag="VHM69_16730"
     CDS             complement(2979..3668)
                     /locus_tag="VHM69_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX2742089.1"
                     /translation="MEFLTGEWLGRLAGVLVVNLILSGDNAVVIAMAARSLEGANRRR
                     AIIWGAAGAVILRLVFAAVITYLLVIPFLQVVGGLLLIWIAWKLIHDDEPEDGKIEAG
                     QSAWQAIRIIIVADAVMSLDNVIALVQAARIDGEVNASLLVIGLATTIPLVVFGAALL
                     TSLLNRFPVLVYIGAGLLVYLAVEMFFADVFLHHYLEPYKSIEWIIGVAAAVIFMAVA
                     WLWAKRSKEPG"
     gene            complement(3757..4776)
                     /locus_tag="VHM69_16735"
     CDS             complement(3757..4776)
                     /locus_tag="VHM69_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742090.1"
                     /translation="MTIIELVLSLAAILGAAILFTNAVEILGDRLNLGAGAVGSLLAA
                     VGTALPETMIPIVAILGALLVGSGGAAASEIGIGAILGAPFLLATLALFIVGAAGLFY
                     RNRRDTGSEITVDRQTARHDLAFFLPCFALAAGAGLVSLPLVLKIILAVVLLGAYVLY
                     VVWTVKQGGEAEEDVPQNLTLWPSRSEAPTWAVATQLLGTIAVMGAGAHFFVSAVEHI
                     SKSVGIPAGLIALVLAPLATELPEKFNSVIWLRDNKDTLAVGNITGAMVFQSTIPVSI
                     GVLFTPWHLDFLSALAAAFAIISSIVFLGFLLRKAPLRAFVMLGAGSLYIVFVVTAVV
                     YEFVL"
     gene            4788..5642
                     /gene="larB"
                     /locus_tag="VHM69_16740"
     CDS             4788..5642
                     /gene="larB"
                     /locus_tag="VHM69_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX2742091.1"
                     /translation="MNVENVLDSYPLANLLHQEAEGMDELEKTLAAVASGDLSPESAA
                     GHLKRGEVRYLDEFAALDLGRSIRKGVPEVVYARGKSPAQVAGICATLLESCERVIVS
                     SPTPEHEAQIRATLPDVPLRQAGRSLVIGSGEPTPSGGRVGAVSAGTSDLPVLEEALA
                     VVGEMGASVMSFHDVGVAGVHRLAVPLQELRQFDPDSMIVAAGMEGALPSVVAGLVSV
                     PVIGLPTSTGYGLGGDGTAAILGMLQTCSPGLSVVNVDNGVGAGATAALIANRAALGR
                     TAHENGRT"
     gene            5657..6193
                     /gene="thpR"
                     /locus_tag="VHM69_16745"
     CDS             5657..6193
                     /gene="thpR"
                     /locus_tag="VHM69_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2742092.1"
                     /translation="MRTFVAIFPPPEVREALFHASCELPASKDFRLVTPEKIHLTLKF
                     LGDVPGDNLTVVTQSLDPVRERHDPFDVSTSGFGAFPSERRARILWAGVGEGSDPLRA
                     LARDVEDLLEPAGFERERRPYMPHLTLGRARGRPAKLDDILATPPAQHFTVSGVDLVR
                     SVPGDDGVTYSVLATYSF"
     gene            complement(6364..6903)
                     /locus_tag="VHM69_16750"
     CDS             complement(6364..6903)
                     /locus_tag="VHM69_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742093.1"
                     /translation="MAELVDNCLHLEGLRVAAGSEYRCPVCEESLDLAGVARWVRDAE
                     KTRDGAYSMVEGDGFEDMVREGLQREVYRRRRVMYELENVPRAVTARPEMILVIHDVA
                     RATYECRIFYKEPRPAGGLDQISVEAVQETILDLRSDPEPIVRLIAEKVEEFHAFRAK
                     LSEAGEPAPERRVFYANEL"
     gene            7285..>7710
                     /locus_tag="VHM69_16755"
     CDS             7285..>7710
                     /locus_tag="VHM69_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2742094.1"
                     /translation="MILNPSAGKGRAEDRRAFVSRFLEERGVRPVWHLTEGPGHAGSI
                     VRELPEDVLVVAVGGDGTVHEVAAACATTGRTMGVLPAGSGNDYVKALGVGTGLRRAL
                     EILVGGKTRVVDMGEVNGVPLNNGLGIGFDAEVAAGVAEA"
BASE COUNT         1550 a         2433 c         2444 g         1283 t
ORIGIN      
        1 caaagcccga tggcaggcag cacagaagga tgcacggcta tcgcgaccca gcagaagaac
       61 gtgaagaggc cgaggtatct ccatttccgg ctctctatga agagcacggc gaagagtagc
      121 gtagcgggta gtatcaggaa ccttgctgcg tacttgtcct ccgagatacg ggctgccagc
      181 tccaccccaa cgttctgtac ggatggttgg atgaagatga tgtgaaacag ggcgtagacc
      241 gaagctgtga ggacggcggc ccgctcgttc ttcaataggg tccttgccag ggcgtagacc
      301 atcaacaggg ctactattac gagcgtcggg gtcaggtatc taagcacaag ttcgatggtg
      361 tccaggccgg agacgcggga gagggcagcc tgctcgagca accacccgtt gaccttggcg
      421 cgagagagct cggcaagctt ctcaccgaag taagggtcgc gcaacgcgag cctctccgcg
      481 ttcgcgaagt ccttcaccca cgacaggtag acccatatgt catcgtagtt gttaggtacc
      541 ctcctgatcc caacgaaagc cagtactcca cccaacaacg cgaacggcgc ccagagccag
      601 ccggcccgtt gaccgtacgg gtcatcctcc accgcgggcg ctttcatgcg aagcgttctc
      661 catgtcgcgc cggcgagaga agcagcaaga agcgcgcccg cggccaagag gtactcccct
      721 atgcttaggt gcaggatcag ggcaggcaca cccaggagcc cgaagacgcc cgtgctgatc
      781 ccgaacccca caggcacgag cgcgaccccg tatatgtcgt ccctgagaaa ccagtgcgaa
      841 atgagcaccc caggcgccgt gaagagcacg agggcagcag agaaggcgac gggagggaaa
      901 gccccggaga cttctctgag tggcaggagc acgatcaccg ccgccgcact agagagcagc
      961 acgagcgcat ccagggacct cagacgactc aagatactac ctcgacgctc ggtagcgaag
     1021 gctcctgcac aacgcgttcc atcggcgaaa gctagcacac tctcctccgt ctctcagccc
     1081 ccgccatacc tggccgttct tccgggtggc gcgcgtatgg tggtgcggta caaatagcga
     1141 atggagatct tcgagaacct ctcggcgagt tcgaggctgg agagcaatga gctcgacgcg
     1201 tactcgcggg ccgtgcggga cgttacggag aggctcgagc cggcggtgat ctcggtcggc
     1261 ctgccggacg gacgcggcgg cgggagcggg gtgatactcg gcttcgacgg tatctcggcc
     1321 acagccatca ccaacagcca cgtcgtgcgc ggactccgga gcaggagggc cggcaacccc
     1381 ggcggcgata tccaggtcac gctctcggac gggacatcgg tacacgctga tgtccttggc
     1441 gacgacccgc cgagcgatct cgccgtcgtc cgcttcgaga ccgaagagga gcctaccgtc
     1501 gccacgctcg gcgatgctgg ggacctcgtg gtcggccagc tcgtcgtcgc gatagggaac
     1561 cctctcggct tccagcgtag cgtcaccgcc ggggttgtga gcgccctcgg aaggtccatc
     1621 aggggcgaga gcggccgtct catagagaac gtgatccaga ccgacgcggc gatcaacccc
     1681 ggtaactcgg gcggccccct ggccgacgcc tcagcgcgcg tcgttggtat caatacggcc
     1741 atcatcgggg gggcgcaggg tataggcttc gccgtgccgg tctccggtac cttccgccgc
     1801 gtggtgttca cgctcgtgac ggagggacgg gtgcgccgcg cctacctcgg ggttgtggtt
     1861 gagagccgcc agccacgcgg cgagttcaag ggcggcgctg gcgtgaggaa catcgccccg
     1921 aacagccctg ccgagagggc cggcgtgaaa cccggagacg tcatcgtcgg cctgaaagga
     1981 gagcccgtcc actcgaacga cgacctcctg aacctgctcg acgcatcgtc aatcggcaag
     2041 gacgccacgc tgcgcgtgct gcgcggtaac agggaactat cgctcaccgt gcgcccgagg
     2101 gaacaaccag cggaataagg cttcaggttt caggcatcag ccagaacgtg ggcggcgaaa
     2161 ctcatagatg acaacgcggg tgccgataga ccaaccttac gtctttagga aggcggcata
     2221 tccgtgtggc actcccgctg cctcgagagg aacctgacgt ctgaaacctg taacctgata
     2281 cctgaagcct gttgccaacc ggagggagga cctatgcccg acgtgacttt tatggtgaac
     2341 gatatcgcgg aggactcgga gaagctggag cgggcactga cgcgtctgga cttcgtggat
     2401 ctcgtgaacg tggattcgga gagggggctc gtcgccgtct cctacgaggg tggggaggcg
     2461 gagatcaaga ggatcgaggg cgccatagaa gaggctggcc atgatgccga gccctctccc
     2521 ggcgccgacc cagacgtgga ctgacccgtg accaccatct ccgtggcgga agcggcccgc
     2581 ttccttgacg ttccggagga ggaagcgcgg aagctggccg acgacgaagg caatgtaccc
     2641 ctggaggtcg tcaggagccg ggcgggcgcc gctgccatcg cgcgcgatct cgccgccgcc
     2701 cgcgaggagg tcgggcggtt atccggccag ttgaaggagg tccgagcctc caacgtgcgg
     2761 ctccagaaag agatcaaaga gtcggtcgag gagcggcggc ggctcacgca agaggtactc
     2821 gagctcaggg cggcggccga ggagcggctc atgctcatgg agcgcatcga gcacatagcc
     2881 cgcgtcgagc aggatctcga ggagagcacc tctgaactgg agcgcctcag gaacaggaga
     2941 ctgcttggac ggctcctgaa ccgctaggag gggacagact accctggttc cttcgagcgt
     3001 ttcgcccata accaggcaac ggccataaag atcaccgcag cggccacgcc gatgatccac
     3061 tcgatgctct tataaggctc caggtagtgg tgcaggaaaa cgtcggcgaa gaacatctcc
     3121 acagccaggt aaacgagcag ccccgccccg atgtagacaa gcacggggaa gcggttaagc
     3181 agcgaagtaa gcaacgccgc gccgaagacg acgagcggta tcgtcgtcgc gagcccgatc
     3241 acgagcagcg aggcgttcac ttcgccgtca atcctcgccg cctggacaag cgcgatcacg
     3301 ttgtccagag acatcaccgc gtcggccacg atgataatcc tgatggcctg ccaggcgctc
     3361 tgaccggcct cgatcttccc gtcctcgggc tcgtcgtcgt ggatgagctt ccaggcgatc
     3421 cagatcagca ataatccgcc aaccacctgc aagaacggga tcaccagaag gtaagtgatg
     3481 acggccgcga agacgagcct gaggatcacg gcgccggccg cgccccagat gatggccctg
     3541 cggcggttgg cgccttcgag gctgcgggcg gccatggcga tgaccacggc gttgtcgccc
     3601 gagaggatca gattgacgac gagaacccca gcgagccggc caagccactc tcccgtcaag
     3661 aattccatct agccccgcat ttccttcgac cgatcacaca acagaacagc tatcttacca
     3721 ggaaagtggt caatcgggcg caggatgaga agcacatcag aggacaaatt catagactac
     3781 cgccgtcacg acgaacacga tgtaaagcga accggcgccg agcatgacga acgcccggag
     3841 cggggctttg cgcagcagga acccgaggaa gactatgctt gagatgatgg caaacgcggc
     3901 ggccagggcg ctcaggaagt cgaggtgcca cggcgtgaac agcaccccga tcgagacggg
     3961 gattgtgctc tggaagacca tcgcccctgt gatgttgccg acggccaggg tgtctttatt
     4021 gtccctgagc cagatcacgg agttgaactt ctcaggcaat tccgtcgcca gaggcgcgag
     4081 gaccagggcg atcaggcccg cagggatgcc cacggatttg gagatgtgct cgacggcgct
     4141 cacgaagaag tgggctcccg ctcccatcac cgcgatagtc ccgaggagct gcgttgccac
     4201 ggcccaggtc ggggcctcgg agcgggacgg ccacagcgtg aggttctgag gtacgtcctc
     4261 ttcagcctct cccccctgct ttacagtcca caccacgtag aggacgtacg cgccgagcag
     4321 caccacagcg aggattatct tcagcaccag gggcagagac acgagacccg ccccggcggc
     4381 aagggcgaag cagggcagaa agaaagcgag gtcgtgtcta gcggtctgcc tatcaaccgt
     4441 gatctcactc cctgtatccc tgcggttccg gtagaatagt cctgccgccc cgacgatgaa
     4501 cagggcgagc gtcgcgagca ggaatggggc gccgagtatg gcgccgatcc ctatctcgct
     4561 ggctgcggct ccacccgagc ccaccagcaa agcgcccagg atggcgacta tcgggatcat
     4621 ggtctcaggc aacgccgtgc cgacggccgc gagcaagctc ccaacggccc cggcgccgag
     4681 gttcaacctg tcgcccagga tctcgacggc gttggtaaac aggatcgccg cgccgagtat
     4741 ggcagccaga gagaggacca attcgattat cgtcacaggt cctccttatg aacgtcgaga
     4801 acgtgctaga ttcttacccg ctggcgaact tactacacca ggaagcggag ggcatggacg
     4861 aactcgagaa gacactggca gcggtagcga gtggcgacct atctccggaa tcggccgcgg
     4921 gacacctgaa aaggggcgag gtccgctacc tggacgagtt cgccgccctc gacctcggcc
     4981 gctctatcag gaagggcgtg cccgaagtcg tctacgcccg cggcaagagc ccggcacagg
     5041 tagccggcat ctgcgcgacc ctgcttgaat cgtgcgagcg tgtgatcgta agctccccca
     5101 ccccggaaca cgaggcacag atccgcgcga cgcttcctga tgtaccgctc cgccaggccg
     5161 gacgctccct ggtcatcggt agcggcgagc ccaccccgag cgggggacgc gtcggcgctg
     5221 tgagcgcggg cacctcggac ctgccagtgc tcgaggaggc gctcgccgtg gtcggagaga
     5281 tgggcgcatc cgtgatgagc ttccacgacg tcggggtggc aggcgtccac cgcctcgccg
     5341 tgccgttgca ggagctgaga cagttcgacc cagactccat gatcgtggcc gcagggatgg
     5401 agggggcgct gcccagcgtg gtcgctggcc tcgtctccgt ccccgtgata gggctcccga
     5461 cctcgaccgg atacggcctc ggcggcgatg ggacggcagc catcctcggc atgctccaga
     5521 cctgctctcc cgggctctcc gtcgtcaacg tggacaacgg cgtcggagca ggggccaccg
     5581 cagcactgat cgccaaccgc gcagccctcg gccgcacggc ccacgagaac ggccgaacgt
     5641 aagaccccgt ctacgaatgc gaaccttcgt cgccatcttc ccgcctcccg aagtcaggga
     5701 agcgctgttc cacgcgtcgt gcgagctgcc tgcgagcaag gacttccgcc tcgtcactcc
     5761 ggagaagatc cacctgacat tgaagttcct gggcgacgtg ccgggtgaca accttaccgt
     5821 ggtgacgcag tcactcgatc cggtgcgcga gagacacgac ccgttcgatg tctcgacctc
     5881 cggcttcgga gcctttccct ccgagaggag agcccgcatc ctctgggccg gcgtcggaga
     5941 agggtcagat ccgctgcgcg ccctggccag ggatgtggag gatctcctgg aacctgccgg
     6001 cttcgagcgc gagcgcagac cctacatgcc ccacctcacc ctgggtcgtg cacgaggccg
     6061 cccggcaaag ctcgacgaca tattggccac tccgcccgcg cagcacttca cggtatcagg
     6121 cgtggacctg gtgaggagcg tgcccggcga cgacggagtc acctactccg tcctggcgac
     6181 gtactctttc tagaaggccg agatcagggg tccgatagac atgagaatga acagcaggat
     6241 caggatcata acgacaaggg atatgaaggt actcccgctc atgcgaccgc gctttctgga
     6301 ttcgtattca tctggcggtc aattctagcc gctagccgac tttgctgcca gcgcccggac
     6361 gcttcagagc tcgttggcgt aaaacaccct gcgctcgggc gcgggttcac cggcctctga
     6421 cagcttcgcc ctgaaagcat ggaactcctc caccttctcg gcgatgagcc ttacgattgg
     6481 ctcagggtcg gagcgaagat ccagaatagt ctcctgcacc gcctccacgc tgatctgatc
     6541 cagacctccc gccggtcgcg gctccttgta gaagatccgg cactcataag tcgccctcgc
     6601 aacatcgtgg ataaccagga tcatctccgg cctcgccgtc actgcccgcg gcacgttctc
     6661 tagctcgtac attaccctcc gtcgccggta gacttcccgc tgtaaccctt cacgtaccat
     6721 atcttcgaac ccgtcacctt cgaccataga gtaagccccg tcgcgggtct tctcggcgtc
     6781 acgaacccac cttgcaacac cggcgaggtc cagactttcc tcacaaaccg gacagcggta
     6841 ctccgatccg gcagcaaccc tcaagccctc aaggtgcagg caattatcga ccagctcagc
     6901 catgtggacc agtattgtac tcctttccct ctcctgcgcg actctcaccc aatgaacgat
     6961 aatgctctat acctgcactt tagctctata tgtatcagaa gtctatggta gtggagaacc
     7021 ttcttgtgac ttcccgcagt tattccggct caggtcgtgc tgcgtttttc ccgcaaagga
     7081 gcctctgttc gcttcggacc gcgcccgcgg acacggtggg gagacaggca tcccgttccc
     7141 tggtggctca tgctgtctgg cgaacagctc atcattctcg ccatagccta tacctcgcgt
     7201 ataggctgaa tgatctccga ccgggccgct gaagtgtagt tctgttattt tccggctgtg
     7261 atgttcggtg gaggagaggc gcatttgatc ctcaacccgt cggccggcaa ggggcgggcg
     7321 gaggatcggc gggcgttcgt ctcgcggttt ctggaggagc ggggggtgcg cccggtctgg
     7381 caccttacgg aaggtcccgg gcatgctggc agcatcgtac gcgagttgcc tgaggacgtt
     7441 ctagtggtgg cggtcggcgg agacgggacg gtgcacgagg tggctgcggc atgcgccacg
     7501 acgggaagga ctatgggtgt gttgcccgcc ggcagcggca acgactacgt caaggcgcta
     7561 ggggttggca cgggactccg gcgggcgctg gaaattctcg tcggaggaaa aactcgcgtc
     7621 gtcgatatgg gagaggtcaa cggggtcccc ttaaacaacg ggctcggcat cggcttcgac
     7681 gccgaggtgg ctgcgggcgt cgctgaggcg
//
LOCUS       DATAIR010000707         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_70105, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000707 DATAIR010000000
VERSION     DATAIR010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70105"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHM69_16760"
     CDS             <1..189
                     /locus_tag="VHM69_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742095.1"
                     /translation="GNKDCYTCVRGGNTRRKLYNGVSRLNLKHPQWAWYSMISVLLTD
                     VYFRLIQAGVIPDLTILG"
     gene            199..1956
                     /locus_tag="VHM69_16765"
     CDS             199..1956
                     /locus_tag="VHM69_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2742096.1"
                     /translation="MQTDANGRREDYEVREHDVLIIGAGGAGLRAAVAAAEHGLSVGV
                     VTKSLLGKAHTVMAEGGMAAALGNVDSDDSWRQHFMDTMKSGRFINNWRMAEIHAKES
                     PDRVYELEQWGALFNRTPEGKISQRPFGGHTYRRLAHVGDRTGLELIRTMQEKVLATD
                     AKVYMETTVTKLFKKDGRVVGALAYTRENGKFVHFKAKAVIMATGGWGRIFKVTSNSW
                     EGTGDGVVLGYDAGAELVDMEMMQFHPTGMVWPPGVRGLLVTEGVRGEGGVLRNSEGK
                     RYMEKYDPEKMELSTRDVVARANYTEVQEGRGSEHGGVYLDITPLGYDGIMKKLPTMY
                     EQFLKLADIDISKEPMEVFPTVHYTMGGIKIDPETAISSVPGLFAAGEVAGGLHGANR
                     LGGNSLSDLLVFGRRAGEGAAAYIEESVHSADIDEGEVLHEIGRVLEPLEKKEEGESP
                     YLIQQQLQEAMMEHANLMRDEESLKEGLGKVLDLKDRLPQISVPGSRLFNPGWHTAQD
                     VRYLIQISEIIVRTALERKERRGAQWRLDFDGLDEELGKINFIVKKDEQGEVGIERRE
                     IPPMPEHLASLLEEEKAKA"
     gene            1953..2765
                     /locus_tag="VHM69_16770"
     CDS             1953..2765
                     /locus_tag="VHM69_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX2742097.1"
                     /translation="MSEGNGNNPGGVTSMAHSPVPDETLGISDKTAKLKIFRGDAEGG
                     EEVEYEIPLVEGMVVLDAVLTIQARQAPDLAVRWNCKAAHCGSCSAEINGRPKLLCKT
                     RVEEYGEGEIHVGPMRAFPVIKDLVSDVSWNYEVSKGIKPFQSSQQAPFTMYEEDVER
                     LYEPKSCIECFMCQDVCHVLRTHHKHPEFAGPRFFVRSQWLEMHPMDEADRLHDLKED
                     NGIGFCNITKCCTEVCPVDIHITDNSIIPLKERVGDEYYDPAKWLMRKIRRR"
     gene            complement(2913..2985)
                     /locus_tag="VHM69_16775"
     tRNA            complement(2913..2985)
                     /locus_tag="VHM69_16775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2950..2952),aa:Gly,seq:ccc)
     gene            3004..>3371
                     /locus_tag="VHM69_16780"
     CDS             3004..>3371
                     /locus_tag="VHM69_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2742098.1"
                     /translation="MSGVAYRFCPRCAGRLEQRVLKEDDPSRLVCEACGFVFYLGPKL
                     VVGAIFELDGGIVLIQRDIEPGYGKWTFPGGFVERGERAEAAAEREVLEETGLEIEVR
                     EIVGLYTYEGEVPAIAVFAAG"
BASE COUNT          729 a          964 c         1152 g          526 t
ORIGIN      
        1 ggcaacaaag actgctacac gtgcgtgagg ggcggcaaca ccaggcgcaa gttgtacaac
       61 ggcgtcagca ggttgaacct gaagcatccg cagtgggcgt ggtacagcat gatctcggtg
      121 ctccttacgg acgtctattt caggctaatt caggcaggcg taatacccga cctgacgatt
      181 ttagggtagg tggtggagtt gcaaacagac gcgaacgggc gccgcgagga ctacgaggtg
      241 cgcgagcacg acgtcctgat catcggcgcg ggaggcgccg gactcagggc ggcggtcgcc
      301 gcggcggagc acgggctctc ggtcggcgta gttacaaaga gcctgctcgg caaggcccac
      361 acggtcatgg ccgagggcgg catggcggcc gcgctcggga acgtcgactc tgacgactcc
      421 tggcgccagc atttcatgga caccatgaag agcggcaggt tcatcaacaa ctggcgtatg
      481 gcggagatcc acgccaagga atccccggac agggtctacg agctcgagca atggggagcc
      541 ctcttcaacc ggacgcctga aggcaagatc agccagcggc ccttcggcgg ccacacctac
      601 cggcggctcg cccacgtcgg cgacaggacg gggctggagc tgatccggac catgcaggag
      661 aaggttctcg ccaccgacgc gaaggtctac atggagacca cggtgacaaa gctcttcaag
      721 aaggacgggc gggtcgtcgg cgctctggcc tatacgcggg agaacggcaa gttcgtccac
      781 ttcaaggcga aggccgtcat catggcgaca ggaggatggg gccgcatctt caaggtcacc
      841 tccaactcgt gggaggggac gggggacggc gtggtcctcg gctacgacgc gggggccgag
      901 ctggttgaca tggagatgat gcagttccac ccgaccggga tggtatggcc tcccggcgtg
      961 cggggattgc tcgtcacaga gggcgtgcgc ggcgagggcg gcgtgctccg gaactccgag
     1021 ggcaagcgtt acatggagaa gtacgacccc gagaagatgg agctctccac gagggacgtc
     1081 gtcgcgcggg caaactacac agaggtgcag gaggggcgcg gcagcgagca cggcggggtg
     1141 tatctggaca taacgcccct cggctacgac ggcatcatga aaaagctgcc gaccatgtac
     1201 gagcagttcc tcaagctcgc cgacatagac atctccaaag agcccatgga agtcttcccg
     1261 accgtccact atacgatggg cggcatcaag atcgacccgg agacagccat atccagcgtg
     1321 ccagggctct tcgccgcggg cgaggtcgcc ggcgggttgc acggcgcgaa ccggttggga
     1381 ggcaactctc tctccgacct gctcgtattc ggaaggcggg ccggcgaggg tgccgcggcg
     1441 tacatcgagg agagcgtgca ctcggcggat atcgacgagg gcgaggtgct gcacgagatc
     1501 gggcgcgtac tggagccact ggagaagaag gaagaaggcg agagcccgta cctgatccag
     1561 cagcagctcc aggaggcgat gatggagcac gcgaacctga tgcgcgacga ggagtctctc
     1621 aaggaagggc taggcaaggt actcgatctc aaggacaggc tgccgcagat aagcgtgccc
     1681 ggatcgaggc tcttcaaccc tggctggcac acggcccagg acgttcggta cctgatccag
     1741 atctcggaga tcatcgtcag gacggccctg gagcgcaagg agcgccgcgg ggcgcaatgg
     1801 cgactggact tcgacggtct ggacgaggag ctggggaaga tcaacttcat tgtgaagaag
     1861 gacgagcagg gcgaggtcgg catcgaacgg cgcgagatac caccgatgcc cgagcacctt
     1921 gcgtcgcttc tggaggagga gaaggctaaa gcatgagcga aggtaacggc aacaacccag
     1981 ggggagtgac ctccatggct cactccccgg taccggacga gacacttggc atcagcgaca
     2041 agacggcgaa actcaagatc ttccgcggcg acgccgaagg cggcgaggag gtcgagtacg
     2101 agatacctct cgtcgagggc atggtcgtcc tcgacgcggt gctcacgata caggccaggc
     2161 aggcccctga cctggctgtg cgctggaact gcaaggcggc ccactgcggc tcgtgcagcg
     2221 cggagatcaa cggcaggcct aagctgctct gcaagacgcg cgtcgaggag tacggcgagg
     2281 gagagatcca cgtcggcccc atgcgggcct tccccgtgat aaaggacctg gtctcagacg
     2341 tctcatggaa ctacgaggtc tccaagggca tcaagccgtt ccagagtagc cagcaggcgc
     2401 cgttcacgat gtacgaggag gacgtcgagc gcctctacga gccgaagagc tgcatcgagt
     2461 gctttatgtg ccaggacgtc tgccacgtcc tgaggacgca ccacaagcac cccgagttcg
     2521 cgggcccaag gttcttcgtc aggagccagt ggcttgaaat gcacccgatg gatgaggccg
     2581 acaggctgca cgacctcaaa gaagacaacg gcatcggttt ctgcaacatc accaagtgct
     2641 gcaccgaggt ctgccccgtc gacatccaca tcaccgacaa ctccattatt cccctcaaag
     2701 agcgcgtcgg cgacgagtac tacgacccgg ccaagtggct gatgcgaaag attagaagaa
     2761 ggtaacaggt ttcaggctac aggaagacaa agggcgagag gtcgctccga ccggcttcgg
     2821 agcgtcttct tcgtttttgc agagatggtc gaagccgcga caaagtaccg aaaggcagaa
     2881 cctgaaacct gtagcctgaa gcctacaacc agagcggacg acgggcttcg aacccgcgac
     2941 ctccagcttg ggaagctggc gctctaccaa ctgagctacg tccgcgtgag gagggatttt
     3001 atcatgtcgg gcgtggcgta caggttctgc cccaggtgcg caggacgtct ggagcaacgt
     3061 gttctgaagg aggatgatcc ctcgcgtctc gtgtgcgagg cgtgcggctt cgtcttctat
     3121 ctcgggccga agctggtagt cggcgcgatc ttcgagctgg acggggggat agtcctgatc
     3181 caacgcgaca tcgagcccgg ctacggcaag tggaccttcc ctggcggctt cgttgagcgc
     3241 ggcgagaggg ccgaggccgc cgcggagcgc gaggtgctcg aagagacggg tttggagatc
     3301 gaggtgaggg agatcgtcgg actctacacc tacgagggcg aggtgccggc tatagccgtc
     3361 ttcgcggccg g
//
LOCUS       DATAIR010000708         6501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_212464, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000708 DATAIR010000000
VERSION     DATAIR010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212464"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VHM69_16785"
     CDS             complement(<1..620)
                     /locus_tag="VHM69_16785"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2742099.1"
                     /translation="MSVDRGVRRRRRPEEAERAILAAARSFLEEHPFREMTVEGVMVR
                     TGLSRPAFYAYFRDRYDLVTRLLEGVGGLLFALDWRWLSGVAGEDESREVLVDALRAG
                     SQTFVEYGPVLRAISDAAGQDARVEEVYRYGLIQRLVTAVAGRISRDVEAGVSPGGLD
                     PEETARALVLMTERYLLDTFGRPERRPSREQTEAVFRTLESVWVNT"
     gene            744..1634
                     /locus_tag="VHM69_16790"
     CDS             744..1634
                     /locus_tag="VHM69_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742100.1"
                     /translation="MERSRMAGDVREVWLRQGIVRYRDVGTGPVLVFVHGVMANGVLW
                     RDVVAALSGRFRCIVPDLPLGGHSVAMRPEADLSPRGVARIVADMMEALELRDVTLVG
                     NDTGGAICQVVVTEHPGRVGRLVLTDCDAYDAFFPVLIGGLFRNAARFLGSRPVDLIA
                     WMLRARFAQRVLFKCVALRRPDRVTLDAYYTSLIQDAGVRRDLTKFLCGVSRKYTMEA
                     ARGFRGFERPVLIVWGDRDLLLPSRLAVRLQHDFPDARLEMVDRSRTFIPEDRPERLA
                     ELIGGFLEENAAAGVRGHSV"
     gene            1718..1945
                     /locus_tag="VHM69_16795"
     CDS             1718..1945
                     /locus_tag="VHM69_16795"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX2742101.1"
                     /translation="MPQTNSQRNDASGNYARFVGLGFTLILIFGLPTVVGFFVDRLAG
                     TLPLFLLIGLALGFVGGMFYVYRALQNLGGG"
     gene            1942..2370
                     /locus_tag="VHM69_16800"
     CDS             1942..2370
                     /locus_tag="VHM69_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742102.1"
                     /translation="MTGHWRLAFRCAGWMFLLAAIAVVVISYLYDKNSAVAFGYGAGV
                     GLLSFVSTAATVTLLTGRSKVVGVVLGVGTFVARYAFVAIALGLPMYRDLWPALPALG
                     GFAGVYFAETVFLVPWSIRTMRFPVAERPVDEVVERRAEV"
     gene            2367..3152
                     /gene="atpB"
                     /locus_tag="VHM69_16805"
     CDS             2367..3152
                     /gene="atpB"
                     /locus_tag="VHM69_16805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX2742103.1"
                     /translation="MIGTLAQISQDELREKILHTFETARHAWHIPLHIGPVDISINKS
                     IWFLWIGAAVTFLILFVGSRLLKDRPGAYQVIVEELYGFGRNLGGQVGEEGRKWFPYT
                     LSLFVFILVLNFIGLIPNTYPVTANISFTLMLALLTFILTQYEGFRRNGAGTYLKSFA
                     PPGLPLKPVMVPFMWGIEVLGEFTKPLTLGMRLYANILAGHLIIYVFLGLILYFGTFI
                     AIVSVPLAVVFYAFEIFVAILQAYIFAILTQVYIELAMFREDH"
     gene            3267..3476
                     /gene="atpE"
                     /locus_tag="VHM69_16810"
     CDS             3267..3476
                     /gene="atpE"
                     /locus_tag="VHM69_16810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX2742104.1"
                     /translation="MIGVGIAAGTAVIGPGAGMGYLIGQTIASIHRQPEAFDQTRTLM
                     FLGIGLVEAFALYGIVFALLIAFVL"
     gene            3489..4034
                     /gene="atpF"
                     /locus_tag="VHM69_16815"
     CDS             3489..4034
                     /gene="atpF"
                     /locus_tag="VHM69_16815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX2742105.1"
                     /translation="MLDPTGIFDLGNTSLIFWEVITFLILLFLLIRYVYPPIRAQIQE
                     RQSQIERSIDEAEKTRAEARELLAEYRRQIEEARGEGRRILEEARKQAEAQRERTKRE
                     AREEGDRIIQRAREEIDREREAAVREVRREVADMVILASEQVIGRSIDRDEHERLISQ
                     ALDDLEAEVAGATAGEGSGTA"
     gene            4031..4570
                     /gene="atpH"
                     /locus_tag="VHM69_16820"
     CDS             4031..4570
                     /gene="atpH"
                     /locus_tag="VHM69_16820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX2742106.1"
                     /translation="MSAVSTYAEALFEAARERDELEEVFSDLQEFVTALRETEELRLF
                     FYGGQIPERQKRRALEGLTEGMSTSTTNFLKVLVDNGREEILDEVLARYEQLVKEHLG
                     RIEVDVTTAVELSEEQRDRLKERLASVLEGREVILETSVDPGLIGGAVFRLGGRLMDS
                     SIRGRLESLREGMLERSVV"
     gene            4570..6159
                     /gene="atpA"
                     /locus_tag="VHM69_16825"
     CDS             4570..6159
                     /gene="atpA"
                     /locus_tag="VHM69_16825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX2742107.1"
                     /translation="MGRLRPEEISSILKGAITDYENRVRTEEVGQVLEVGDGIARVHG
                     LTNVMYQEMVEFEDARGEKIIGLALNLEEDNVGVIVAGEDTHIQEGNTVRRTEQLASV
                     PVGDGVLGRVIDALGRPMDGKGEVEADETLPVEIVAPGIIKRQPVKQPLQTGIKAIDS
                     MIPIGRGQRELIIGDRKTGKTSILLDTIINQADQDVKCVYVAIGQKDSTVAGVVAALE
                     EAGAMEYTTVVNASAAQSATLQYLAPYAGCAIGEYFMNRGQDALLIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAAKLSEDAGAGSLTALPVIETKAGDISAY
                     IPTNVISITDGQIFLDSDLFNSGQRPAIDTGNSVSRVGSSAQIKAMKSVAGTLRLDLN
                     QYRELASFAQFGSDLDRATQQTLNRGERLTAILKQDERDPMPVEEQVVVLYAASNGYL
                     DEVDVEAVTDWERQFRDFMRNSHSDLMDSIRSEQQLSDENEKKLREAVEHFNSNYEPE
                     HTSLVDVSSGDEEENGEEEGS"
     gene            6163..>6501
                     /locus_tag="VHM69_16830"
     CDS             6163..>6501
                     /locus_tag="VHM69_16830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX2742108.1"
                     /translation="MASARAFQRQINTVKNQARVFSALQTVSSVKFRKAEARVRRARP
                     YAENVEEMMRDVAGSASGDLPLLTGREVQRVAICTLTADRGLAGGFNAQVLRRTVSLR
                     NEKDSDALQVA"
BASE COUNT         1261 a         1925 c         2090 g         1225 t
ORIGIN      
        1 aacgtattga cccaaaccga ttccagggtg cggaaaacgg cctcggtctg ctcacgcgac
       61 ggacggcgtt cgggacgccc gaaggtgtcg agcaggtagc gctcggtcat caacaccaac
      121 gcccgcgcgg tctcttcggg atcaaggccc ccaggcgaga cgccagcctc tacgtcgcgc
      181 gagatgcggc cggccacagc cgtgacgagg cgttggatga gaccgtacct gtagacttcc
      241 tcgaccctgg catcctgccc cgcagcgtcg gagatcgcac gcagcacggg cccgtactcg
      301 acgaaggtct gagaacccgc ccgcagcgca tccaccagaa cttccctcga ctcgtcttcg
      361 ccagccacac cggatagcca gcgccagtcc agcgcgaaga gtagcccgcc aacaccctcg
      421 agcagacgcg tgaccaggtc gtaacggtcc cggaaatacg cgtaaaaggc gggccgggac
      481 aagccggtac gcaccataac cccctccacg gtcatctccc tgaaaggatg ttcctccaga
      541 aatgaccgcg cagcagccag tatcgcccgc tcagcctcct caggcctgcg cctcctgcgc
      601 acaccccgat ccacactcac ctcaactcct caacctactc aagtcgatac cagacaattt
      661 accgcgttat tgacaccgtg tctattgacg tcatgtcaat aacgggctat tatctggata
      721 agaagccacg agaggagagc gacatggagc gcagccggat ggcgggtgat gtaagggaag
      781 tttggctgcg gcagggtatc gtccgataca gggatgtcgg gactggtccg gtcctcgtct
      841 tcgtgcacgg tgtaatggcg aacggggtac tttggcggga cgtggtggcg gctttatcgg
      901 ggcggtttcg gtgcattgtc cctgaccttc cgctcggcgg acattccgtg gcgatgcggc
      961 ctgaggctga tctaagccct cggggcgtgg cccgcatcgt cgctgacatg atggaggcgc
     1021 tcgagctgcg cgacgtgacg cttgtcggca acgacacggg cggagccatc tgccaggtag
     1081 tcgtcaccga gcaccccggg cgcgtcgggc gtctcgtcct gaccgactgc gacgcctatg
     1141 acgcgttctt ccctgttctg atcggcggcc tatttcgcaa cgctgcgagg ttcttgggct
     1201 cgcggcctgt tgaccttatc gcgtggatgt tgagggctcg attcgcgcag cgggtgctat
     1261 tcaagtgtgt cgccctacgg cgcccggaca gggtcacgct cgacgcttac tacacatctc
     1321 tcatacaaga cgccggcgtc aggcgagacc tcactaaatt cctatgcgga gtgtcgagga
     1381 aatacacgat ggaggccgcg cgcggtttcc ggggcttcga gcgtcctgtc ttgatcgtgt
     1441 ggggggacag ggatctcttg ctcccctcac gcctcgccgt ccgcctgcaa cacgacttcc
     1501 ctgatgcgcg gttggagatg gttgaccgct cgcggacctt catcccagaa gacaggccgg
     1561 aaagattggc ggagctaatc ggcggcttcc tcgaagagaa cgccgcggcg ggcgtacgtg
     1621 gtcacagcgt atagccggcc acggtctttc gggggaagag tcaccccgag cttgacactg
     1681 attgcaacaa attggcccgt aaggtatagt ttccgtgatg ccccagacca actctcaaag
     1741 gaacgacgcg agtggcaact acgctcgttt cgtcggtctt ggattcactc ttatcttgat
     1801 cttcggccta cccacggtgg taggtttctt cgtggacagg ctggcgggga cgttgcctct
     1861 gttcctcctg atcgggctcg cgctcgggtt cgtcgggggt atgttctacg tgtacagggc
     1921 gctccagaat ctggggggcg gatgaccggt cactggcggc tggctttccg gtgcgccggc
     1981 tggatgtttc tgctggcggc cattgccgta gtagtgatct cctacctcta cgataaaaac
     2041 tccgctgtcg ccttcggcta cggtgcgggg gtgggtctct tgagcttcgt ctctacggcg
     2101 gcaacggtca cgttattgac cgggaggtcg aaggtagtcg gagttgttct tggggtaggt
     2161 actttcgtgg cgcgatacgc gttcgttgcg attgcgctcg ggctacccat gtacagggac
     2221 ctgtggccag cgcttcctgc gctgggtggg tttgccgggg tatatttcgc ggagaccgtg
     2281 ttcctcgtgc cgtggtcgat caggacgatg agatttccgg tcgcggagcg acctgtcgat
     2341 gaggtagtag aacggagggc ggaggtttga taggtacgct tgcccagatc agccaggacg
     2401 agctaagaga gaagatcctg cacaccttcg agaccgccag gcacgcctgg cacataccgt
     2461 tgcacatcgg cccggtagac atctcgatca acaagagcat ctggtttctg tggatcgggg
     2521 cggctgttac gttcttgatc cttttcgtag gaagccggtt gctcaaggac aggcccggcg
     2581 cttaccaggt gatcgtcgag gagctatacg gcttcgggcg caacctcggt gggcaggtcg
     2641 gggaggaggg gaggaagtgg ttcccttaca ccctctcgct cttcgtgttc atcctggtgc
     2701 ttaactttat tggcctcata cccaacacct atcccgttac ggcgaacatc tcctttacgt
     2761 tgatgctcgc gttgctcacg ttcatcctca cccagtacga gggcttcaga cgcaacggcg
     2821 cggggactta cctcaagagc ttcgcgccac cggggctgcc gctcaagccg gtcatggtgc
     2881 ctttcatgtg ggggatagaa gtcttggggg agttcaccaa gccactcacg ctagggatgc
     2941 ggctctacgc caacatcctg gcggggcacc tgatcatcta cgtcttcctc gggctcatcc
     3001 tgtacttcgg gactttcatt gcgatagtct ccgtgccgct ggccgtagtg ttctatgcgt
     3061 tcgagatctt cgtcgccatt ttgcaggctt acattttcgc tatactcacg caggtttaca
     3121 tcgagttggc aatgttccgt gaggatcact aaaagtcgta gtcaagtcaa gagagtcagc
     3181 cgagaggcga aaggagtaag aggatggaca tactggcgtt aataatgccc gcactagcgc
     3241 tgcttcaggc aagtggggac gtaaccatga tcggggtagg catcgctgcc ggtacggcgg
     3301 tcatcgggcc cggggccggg atgggctatc tgatcgggca gaccatcgcg tccatccaca
     3361 ggcagccgga ggcgttcgac cagaccagga ccctcatgtt cctcggtatc ggccttgtcg
     3421 aggcgttcgc gctttacggc attgtcttcg ccctcctcat agccttcgtc ctctagagag
     3481 gagagcacat gctcgacccg acggggatct tcgatctggg caacacgagc ctgatcttct
     3541 gggaggtcat taccttcctg atcttgctgt tcttgctcat caggtacgtc tacccgccga
     3601 tccgggctca aattcaggaa cggcagtccc agatcgagcg ctcgatcgac gaggctgaga
     3661 agacgcgcgc cgaggcacgc gaactcctgg ccgagtacag gcgccagatc gaggaagccc
     3721 gcggcgaggg aaggcggatc ctggaagagg cgcgcaaaca ggccgaggcc cagcgcgagc
     3781 gtacgaagag agaggccagg gaagaaggag accgtattat ccagcgcgcc cgtgaggaga
     3841 tagaccgaga gcgggaggcg gccgtgcgcg aggtccgtcg cgaggtggcc gacatggtca
     3901 tcctcgcctc cgagcaggtc atcgggcgct ccatagaccg cgacgagcac gagcgactga
     3961 tctcccaggc cctcgacgac ctggaagccg aggtcgccgg cgcgaccgcc ggtgaaggga
     4021 gcgggacagc ttgagcgccg tctccacgta cgccgaggcc ctcttcgagg cggccaggga
     4081 gagagatgag ctcgaggagg tcttctcgga tctccaggag ttcgttacgg ccttgcggga
     4141 gaccgaagag ctcaggctct ttttctacgg cgggcagatc cccgagcgcc agaagcgcag
     4201 ggccctggag ggcctgactg aaggcatgtc aacctcaacg accaacttcc tcaaggtgct
     4261 cgtggacaac gggcgcgagg agatcctgga cgaggttctc gcccgatacg agcagctcgt
     4321 gaaagagcac ctcggcagga tcgaggtcga tgtaacgacc gcggtggagc tctcggaaga
     4381 acagcgcgac aggttgaagg agcggcttgc gagcgtgctc gaaggcaggg aagttatcct
     4441 ggagaccagt gttgaccctg gccttatcgg tggcgcggtc ttccggctcg ggggacgatt
     4501 gatggacagc agtatccggg ggcggttgga gagcctccgg gaggggatgc tcgagaggag
     4561 tgttgtttag tgggtaggct caggccggag gagatctcct ccatactcaa aggcgctatt
     4621 acggattatg agaaccgcgt caggaccgag gaggtcgggc aggtcctcga ggtgggggac
     4681 ggcatcgctc gcgtccacgg cctgaccaac gtgatgtatc aggagatggt ggagttcgag
     4741 gacgccaggg gagagaagat catcggtctc gctctcaacc tggaggagga caacgtcggc
     4801 gtcatcgtcg cgggcgagga cacccacatc caggagggca ataccgtaag gcgcaccgag
     4861 caactggcga gcgtgcccgt tggcgacggc gtgctgggcc gggtcatcga cgccctcggg
     4921 cgcccgatgg acggcaaggg tgaggtcgag gccgatgaga ccctcccggt ggagatcgtt
     4981 gcgcctggca ttatcaagcg ccagccggta aaacagccgc tccagacggg catcaaggct
     5041 atagactcaa tgatcccgat cgggcgcgga cagcgggaac ttatcatcgg agacaggaag
     5101 acgggcaaga cctcgatcct gctcgacacc atcatcaacc aggccgacca ggacgtgaag
     5161 tgcgtctacg tcgccatcgg ccagaaggac tccacggtgg ccggcgtcgt cgccgctctg
     5221 gaggaggcag gcgcgatgga gtacaccacc gtcgtcaatg cctcggccgc ccagtccgcg
     5281 acgctgcagt acctggctcc gtacgcaggg tgcgccatcg gcgagtactt tatgaacagg
     5341 ggccaggacg cgctcctgat ctacgacgac ctctccaagc acgccgtcgc ctacaggcag
     5401 atgatgctgt tgctcaggcg tccgccaggc cgcgaggcgt atcctgggga cgttttctac
     5461 ctccactctc gccttctgga gcgcgcagcg aagctctccg aagatgcagg agctggttcg
     5521 ctaacggcgt tgcctgttat cgagaccaag gcgggagaca tctccgcgta catcccgacc
     5581 aacgtcatct ccatcactga cggtcagatc ttcctcgatt cagacctatt caactcgggc
     5641 caacgccctg ccatcgacac gggcaactcc gtgagccgcg tggggtcatc ggcccagatc
     5701 aaggccatga agagcgtcgc gggcaccctg cgtctcgacc tgaaccagta ccgtgagctt
     5761 gccagcttcg cccagttcgg cagcgacctg gacagggcga cgcagcagac gcttaaccgc
     5821 ggtgagcgtc tgacggcgat cctgaagcag gacgagcgcg acccgatgcc ggtggaggag
     5881 caggtggtcg tcctgtatgc cgcttcgaac ggctatctgg acgaggtgga tgtggaggca
     5941 gttactgact gggagcgaca gttccgcgac tttatgcgca acagccacag cgacctgatg
     6001 gattccatcc gctccgagca gcagctctcc gacgagaacg agaagaagct gcgcgaggct
     6061 gtagagcatt tcaactccaa ctacgagccc gagcacacga gcctcgtcga cgtctcctcc
     6121 ggggacgaag aggagaacgg cgaggaagag gggagctaga gcttggcttc cgccagggcc
     6181 tttcagaggc agatcaacac cgtaaagaac caggcaaggg tcttctctgc ccttcagacg
     6241 gtatcctcgg tgaagttccg caaagcggag gccagggtca ggcgggcgcg cccatacgcc
     6301 gaaaacgtcg aggagatgat gcgcgacgtg gctggcagcg cgtccggaga ccttccgctg
     6361 cttacaggcc gtgaggtaca gcgggtagcc atctgcaccc tcacggccga ccgcggcctg
     6421 gccggcgggt tcaacgcgca ggtgctccgc aggaccgtca gcctcagaaa cgagaaggac
     6481 tccgacgccc tgcaggtcgc g
//
LOCUS       DATAIR010000709         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_350247, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000709 DATAIR010000000
VERSION     DATAIR010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350247"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1162
                     /locus_tag="VHM69_16835"
     CDS             119..1162
                     /locus_tag="VHM69_16835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase family protein"
                     /protein_id="HEX2742109.1"
                     /translation="MEKIYYEPRVRDYARGREVLERFPDAERVEVPSHWNIPELHGDP
                     GGVGNWTRNKKTVLVLGVKKGLGIRSFYRSADFIAPSQANGCAMACGYCYVGRRKGYA
                     NPITTFVNAGEIMDAVERHATRQGFKFEGTQADDDLWVYEFGTNSDLSVDAALSDNVR
                     DAVSLFRELPNAKATFATKFVNRELLDYDPQLKTRLRFSLMPSEISKLVDVRTSPVER
                     RVAAINDFVEAGYEANVNFGPVIFEDAWLEDYAELFGMLDAALSSEAKEQLAAEIIFL
                     THTRELHEVNLSWHPEGEKVLWRPDIQEHKTSQASGERVLRYERNLKRGLVREFRALL
                     SDKMPYCKVRYAF"
     gene            1166..1669
                     /locus_tag="VHM69_16840"
     CDS             1166..1669
                     /locus_tag="VHM69_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742110.1"
                     /translation="MTYSTGQAGEVLVPVGAPEGFVEHAYQKTFLVDHHISRVWAWLN
                     DPATFVDGQVWPFKVEFVNGGFEPGVLNVHHGPFLNAAGIIGEVRGPGDEPAYRDLRY
                     FYGSYALSPRLARPTRLQFWAGEGGGRTVVTLRLDALVRRRFVPVWHFGQRVFWSRFP
                     RWVDSAL"
BASE COUNT          319 a          545 c          598 g          337 t
ORIGIN      
        1 gcggcggggg ttttaagtgt tgcgggttac tgtgagagag gtgtgtctcc cgtagagttt
       61 cgtcgtggga gagagcacca gagataaagt acccccggtc gcgggcggat tgttgcgggt
      121 ggagaagatc tattacgagc cccgcgtgcg ggattacgcg cggggacgcg aggtcctgga
      181 gcgcttcccg gacgcagagc gtgtcgaggt gccttcgcac tggaatatac ctgagcttca
      241 cggggacccc ggcggtgtgg ggaactggac ccgcaacaag aagaccgtgc tggtgctcgg
      301 cgtcaaaaag gggctcggca tccgctcctt ctaccgcagc gcggacttta tagcgccctc
      361 gcaggcaaac ggttgcgcga tggcctgcgg ctactgctac gttggacgcc gcaagggcta
      421 cgccaacccg atcacgacct tcgtcaacgc cggggagatt atggacgccg tcgagcggca
      481 cgcgacgaga cagggtttca agttcgaggg gacgcaggcc gacgacgacc tctgggtcta
      541 cgagttcggt acgaacagcg acctctcggt cgatgcagcc ctttcggaca acgtgcggga
      601 cgcggtaagc ctctttcgcg aactgccgaa cgccaaggcg accttcgcca cgaagttcgt
      661 caaccgggag ttgctggact acgatccgca actcaagacg cgcctgcgct tctcgctcat
      721 gccgtctgag atctcgaagc tcgtagacgt gcgcacttcg ccggtagagc ggcgcgtcgc
      781 ggccatcaac gatttcgtcg aggcgggcta cgaagccaat gtgaacttcg ggccggtgat
      841 cttcgaggat gcctggctcg aggattacgc cgaactcttc gggatgctcg acgccgcgct
      901 ctccagtgag gcgaaggagc agttggcggc cgagatcatc ttcctcaccc acacgaggga
      961 gttgcatgag gtgaacctct catggcaccc ggagggagaa aaagtcctct ggcggccgga
     1021 catccaggag cacaagacct ctcaggcgag cggcgagcgc gtgctccgct acgaacggaa
     1081 cctgaaacgc ggcctcgtcc gcgagttccg cgcgctgctc tcggacaaga tgccgtactg
     1141 caaggtacgg tacgctttct agaccttgac ctactctacc ggacaggctg gtgaggttct
     1201 ggtgcccgtg ggcgccccgg agggcttcgt cgagcacgcc taccagaaga cgtttctcgt
     1261 ggaccaccac atcagccgcg tgtgggcgtg gctgaacgat ccggccacgt tcgtcgacgg
     1321 ccaggtctgg cccttcaaag tcgagttcgt gaacggtggc ttcgagccgg gcgtgctgaa
     1381 cgttcaccac ggtccctttc tgaacgccgc gggcatcatc ggcgaggtac ggggaccagg
     1441 agacgaaccc gcctaccggg acctgagata cttctacggc tcctacgccc tcagcccgcg
     1501 cctcgcccgc ccgacccgcc tccaattctg ggccggggag gggggagggc gaaccgtagt
     1561 caccctgcgc ctcgacgccc tcgttcgccg acggttcgtg ccggtgtggc actttgggca
     1621 gcgggtcttc tggtctagat ttccacgctg ggtggattcc gccctgtaga ggactgcttc
     1681 gagcttctct ctacgctggt cctccgagcg ccagggcgaa aaggtcttcg tcgtcggtgt
     1741 agaggtccag tagttcgagg ctggcctctt cgaaggttcg ggcggcggat tccggggtg
//
LOCUS       DATAIR010000710         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_350322, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000710 DATAIR010000000
VERSION     DATAIR010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350322"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VHM69_16845"
     CDS             complement(<1..1176)
                     /locus_tag="VHM69_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX2742111.1"
                     /translation="MTEPAANGRGRALITGAGAVTPLGTGVENFWDAALHGKSGIGEI
                     TLFDPSPYPSRIAGECTDFDPTDFISKKQARRMDRFAQLGIAAGIQAAESAGIVDLIR
                     ETPERVAIVMGSGIGGLSTWEREYRILHERGPGRVSPFLITMMVPNMAAGQLAIVLGA
                     TGPSQCPVLACATGGEAIAEGLELIRRGSADVVIAGSSEAPITPLSMAGFCAIRAVSH
                     RNDEPSRASRPFDEGRDGFVMSEGAGAVVLESEEHAERRGAEPISAVAGYGRTTDAYH
                     VTAPDEEGRGVERAMVAAFQDSGLNAEEVGHVNAHATATPTGDGPETRAISRVVPHAL
                     VSGTKSMTGHSFGAVGATEGIMCALALKHKIVPPTINLENLAEDCEELNYVGSEPVEA
                     "
     gene            complement(1173..1418)
                     /gene="acpP"
                     /locus_tag="VHM69_16850"
     CDS             complement(1173..1418)
                     /gene="acpP"
                     /locus_tag="VHM69_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2742112.1"
                     /translation="MDREEVLGKIQEITADRLGVDEGDVTPDASFREDLEADSLDLVE
                     LIMELEEQFGMEIPDEEAEKITTVEEAVDYVLEHQAA"
     gene            complement(1491..2237)
                     /gene="fabG"
                     /locus_tag="VHM69_16855"
     CDS             complement(1491..2237)
                     /gene="fabG"
                     /locus_tag="VHM69_16855"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX2742113.1"
                     /translation="MIGEGRVALVTGGGRGIGRAVAVRLGKEGADVAISYRSNDATAE
                     EAAEEVRAAGGRCEIFKGDVASPEDVEALFKGVAEAFGPVGILVNNAGVTRDNLMLRM
                     KEEEFDDVLRTNLKGTYLCTRAALRPMIRARWGRIISVSSVVGIVGNAGQANYAASKA
                     GIIGFTKSVAREVAQRGITANVVAPGYVETELTGSLPDKVKDQIRGQVPAGRFGEAEE
                     VAEVVAFLAGEGAGYVTGQTVAVDGGMTMQ"
     gene            complement(2234..2683)
                     /gene="fabZ"
                     /locus_tag="VHM69_16860"
     CDS             complement(2234..2683)
                     /gene="fabZ"
                     /locus_tag="VHM69_16860"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX2742114.1"
                     /translation="MKAPLGPAQIRDLIPHRYPFLLVDRIEELEAGVRAVGVKNVTQN
                     EPFFQGHFPDYPVMPGVLIIEAMAQVGAVGVMAGGEHRDKLALFAGIDGVRFRRQVLP
                     GDVLTMEVEITRLKGKVGRGKARATVGDERVCEAELMFAFAERGEVQ"
     gene            complement(2680..3507)
                     /locus_tag="VHM69_16865"
     CDS             complement(2680..3507)
                     /locus_tag="VHM69_16865"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2742115.1"
                     /translation="MQRSETQRAIVFPGQGTAGGEIGGAVRDAVLDCVGDEKVPYQLS
                     VFASSVDLFYRLRKAGIEADVVAGHSLGEYAAAHAAGSLGLHDGVRLVAERDRLMNEA
                     SRENPGGMVALIGADPEEVARATEGAEGVAVAANFNTPRQTVISGEKNALEEVTEHVR
                     GRKVRLEVAGAFHSPLMAEAARRMEDLLEGVAFKVPEIPMVGATDGVLLTTADEVREA
                     LRNQMLSPVRWVAVVERLIQMEVREIFEAGEDGTLTRMLRDFKRKDIEGRTAKDVLS"
     gene            complement(3509..>4470)
                     /locus_tag="VHM69_16870"
     CDS             complement(3509..>4470)
                     /locus_tag="VHM69_16870"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX2742116.1"
                     /translation="GKAGVNGVPAGRILGVGRALGSRVVTNADLESALDTTDEWISTR
                     TGIKERRFVAEGENAVTLAIEASKKALEHAEVEGGSVDLVICGTSTAPESMPSVACLV
                     GEEIGAPGAGAMDVAAACAGFSYAASVASSMIHSGAAGRVLLIGADAMSTIVDQSDRS
                     TAVLFGDGAGAVVLDAGDSDSGFVDHILGADGRGAPLGRAGHPGDDEKLFQNGREVFR
                     FAVKILPDMVEKLISRNGLSPDEVQYVIPHQANARIIRSAMKKMEVPEDRVVINLDRF
                     GNTSAASIPLSYPDIYDEMEKGRYIITVGFGFGLTWAANLYRI"
BASE COUNT          715 a         1602 c         1292 g          861 t
ORIGIN      
        1 cgcctcaacg ggctcgcttc cgacgtagtt aagctcctcg cagtcttcgg ccaggttctc
       61 caggttgata gtcggtggga cgattttgtg cttgagcgcg agagcgcaca tgatcccttc
      121 cgtggcgccg acggccccga aggagtgccc cgtcatggac ttcgtgcctg ataccagggc
      181 gtgcggcact accctggata tagccctcgt ctcaggcccg tctccggtcg gggtggccgt
      241 cgcgtgggcg ttgacgtgcc caacttcctc cgcgttgagg ccggaatcct ggaacgccgc
      301 gaccatcgcg cgctcgacgc ctcggccttc ctcgtcgggc gcggtcacgt ggtaggcgtc
      361 ggtggtacgg ccgtagcccg cgaccgcgga gatcggttcc gcgccgcggc gctcggcgtg
      421 ctcctcgctc tccagaacga ccgcgccggc gccctcgctc atcacgaatc cgtcgcggcc
      481 ctcgtcgaag ggacggctcg cgcgggacgg ctcgtcgttg cggtggctca cggcgcgtat
      541 cgcgcagaag ccagccatcg agagcggcgt tatgggggct tccgaagagc ccgcgatcac
      601 cacgtccgca cttccgcgcc ggatcagttc aagaccctca gcgatagctt cccctccggt
      661 ggcgcaggcc agtacaggac actggctcgg ccccgtcgcg ccgagcacga tcgcgagttg
      721 tccagccgcc atgttgggga ccatcatcgt gataaggaac gggctaaccc tgccaggtcc
      781 tcgttcgtgc aggatacggt actcgcgctc ccaggtcgag agcccaccga tgccgctgcc
      841 catcacgatc gccacccttt cgggggtttc acgaatgagg tccactatcc ccgcgctctc
      901 cgcggcctgt atgccggccg cgatgccgag ctgggcgaag cggtccatcc tgcgagcctg
      961 cttcttgctg atgaagtccg tcggatcgaa gtccgtgcac tcacccgcga tccgcgaagg
     1021 ataaggcgag ggatcgaaga gcgtgatctc ccctatcccg ctctttccgt gcagggccgc
     1081 atcccagaag ttctccaccc cggtccccag gggcgtgacg gccccggcgc cggttatgag
     1141 cgcgcgcccc ctaccatttg cggcgggttc ggtcacgcgg cctgatgctc caacacgtag
     1201 tcaacggcct cttcgaccgt ggtgatcttc tcggcctcct cgtccgggat ctccatcccg
     1261 aactgctcct caagctccat gatgagctct accagatcca gggagtcagc ctccaggtcc
     1321 tcgcggaagg aagcgtcggg tgtaacgtca ccctcgtcta ctccgagcct gtcggcggtg
     1381 atctcctgta tcttgccgag tacttcttcg cggtccaatc cgtctctcct ctcgaagtct
     1441 tgctactttc aacccatcca acgatagcgc gtagtgtaac cgaaaatcgg ctactgcata
     1501 gtcatgccgc cgtccacggc cacggtctgg cccgtaacgt atccggctcc ctcacccgcc
     1561 aggaacgcaa cgacctcagc cacctcctcc gcttccccga acctgccggc aggtacctgg
     1621 cccctgatct ggtccttcac tttgtcgggc aagctgcctg tgagctcggt ctcgacgtac
     1681 ccgggcgcca ctacattggc cgtgatgccg cgctgcgcca cctccctcgc cacgctcttg
     1741 gtgaagccga tgatcccagc cttggaggcg gcgtagtttg cctgccccgc gttccccaca
     1801 atcccgacga ccgaggagac gctgatgatc cttccccacc tcgcccgtat catcggcctt
     1861 aatgcggccc gcgtgcacag gtaagtacct ttgaggttgg tcctcaatac gtcgtcgaac
     1921 tcttcttctt tcatgcgcag catcaggttg tccctggtaa cgccggcgtt gttgacgagg
     1981 attccgacgg gcccgaacgc ctcagccacg cccttgaaca gcgcctcgac gtcctccggc
     2041 gaggcgacgt cgcccttgaa gatctcgcaa cgccctcctg ccgcccgcac ttcctcggca
     2101 gcctcttcgg cagtcgcgtc gttggaacgg taggagatcg ccacgtcggc gccctctttg
     2161 ccgaggcgaa cggccacggc gcggccaatg ccgcgtcctc cgccggtgac gagggcgacg
     2221 cgtccttccc cgatcactgg acctcccccc tctccgcgaa ggcgaacatg agctcggcct
     2281 cgcagaccct ctcgtccccg accgtcgccc tggccttgcc ccgtcctacc ttgcccttga
     2341 gccgtgttat ctcgacctcc atcgtgagca cgtcgccggg caggacctga cgtcggaacc
     2401 gtaccccgtc gattccggcg aagagcgcga gcttatccct gtgctcgccg cccgccatga
     2461 cgccgacggc ccccacctgt gccatagcct cgatgatgag cacgccgggc atgacagggt
     2521 aatcagggaa gtggccctgg aagaatggct cgttctgggt gacgttcttt acgccgacgg
     2581 cgcgcacacc ggcttcgagc tcctcgatcc tatctacaag gaggaacggg taacggtgcg
     2641 ggatgagatc ccggatctgc gccggcccca gcggcgcctt cacgagagca catccttcgc
     2701 tgtccggccc tcgatgtctt ttcgtttgaa gtcccgcagc atcctggtca gagtcccatc
     2761 ctccccggcc tcgaagatct cacgcacctc catctgaatg agacgttcga ccacggccac
     2821 ccaccttacg ggtgagagca tctggttccg caacgcctcc cgtacttcgt ccgcagtcgt
     2881 caggagcacc ccatccgtcg cccccaccat cggtatctcc ggtactttga acgcgacccc
     2941 ctcgaggagg tcctccatcc gccgcgcagc ctctgccatg agcggcgaat ggaaggcccc
     3001 ggctacttcc agccgtacct tgcgaccgcg gacatgctct gtcacttcct caagcgcatt
     3061 cttctccccc gagatgacgg tctggcgtgg cgtattgaag ttcgccgcca cggcgacacc
     3121 ttcagcgccc tccgtagccc tggcgacctc ctccgggtca gcgccgatca aggcgaccat
     3181 ccctccgggg ttctcccgcg aggcctcgtt catcagtcgg tctctctctg cgacgagcct
     3241 tacgccatca tggagtccca ggctccctgc ggcgtgggct gccgcgtact cgccgagcga
     3301 gtggcccgcg accacgtcag cttctatccc ggccttccgc aaacggtaga agagatccac
     3361 ggagctcgcg aagacggaga gctggtacgg taccttttcg tcgccgacgc aatcgagcac
     3421 ggcgtctcgc acggcgccgc cgatctcacc tccggccgtt ccctgtccag ggaagacgat
     3481 cgcccgttgc gtctcgctcc gctgcaaact agatcctgta caggttcgct gcccaggtca
     3541 ggccgaaccc gaagccaacg gtgatgatgt acctgccttt ttccatctca tcgtagatgt
     3601 cggggtacga gagagggatg ctcgccgccg atgtgttccc gaacctgtcc aggttgatga
     3661 ccacgcggtc ctcaggcact tccatcttct tcatagccga acgtattatg cgtgcatttg
     3721 cctggtgggg gatgacgtac tggacctcgt ccggcgagag gccgttgcga gagatgagct
     3781 tctcgaccat gtcaggcagt atcttgacag cgaacctgaa gacctcgcgg ccgttctgga
     3841 agagcttctc gtcgtcgccg gggtggcccg cgcgcccgag cggggctccc ctgccgtcgg
     3901 cgccgaggat gtggtcaacg aagccagaat cgctgtcgcc cgcatcgagt acaacggcgc
     3961 ctgcgccgtc gccgaagagg acggcggtcg aacggtcact ctggtcgacg atcgtgctca
     4021 tcgcgtcggc cccgatcagc aagactctgc ccgccgcacc tgagtgaatc attgatgagg
     4081 cgaccgaagc ggcgtatgag aagcctgcgc atgcggccgc aacgtccatc gcgcctgcac
     4141 caggcgctcc gatctcctcc ccgacgagac acgcgacgga aggcatcgac tcaggcgctg
     4201 tcgacgtgcc gcagatgacg aggtcaaccg accctccctc gacctccgca tgttcgagcg
     4261 ccttcttcga ggcttcgatg gcgagcgtca cggcgttctc gccttcagcg acgaagcggc
     4321 gctctttgat ccccgtgcgc gtcgagatcc actcgtccgt cgtgtcgagt gcgctctcaa
     4381 ggtcagcgtt ggtgacgacc ctgctaccga gggcgcgacc aacgcccaat atcctgcccg
     4441 ccggaacgcc gttcacgccg gccttccctc
//
LOCUS       DATAIR010000711        16892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_350341, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000711 DATAIR010000000
VERSION     DATAIR010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16892
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350341"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..819
                     /locus_tag="VHM69_16875"
     CDS             292..819
                     /locus_tag="VHM69_16875"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2742117.1"
                     /translation="MTFPKVVLAEYDPGWPALFEAEKKRILDAIGLRVLAIEHVGSTA
                     VPGMMAKPIIDVMVGTRNVAATADACTAPLQNIGYASVPLPAIMDRRFFKKGAWLLEC
                     MYHLHMVELGGETWHRYTLFRDVLRAYPDVARSYQELKQQLAVEYRFDRAAYTAAKNW
                     FVEGVIARAENEFKS"
     gene            complement(880..1071)
                     /locus_tag="VHM69_16880"
     CDS             complement(880..1071)
                     /locus_tag="VHM69_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742118.1"
                     /translation="MGLASVAQLFLKHLDCPADAQQGLPHRSGLRLKLIECLLTTFEL
                     GFQLSESLLESLSHRSTFL"
     gene            1141..1917
                     /locus_tag="VHM69_16885"
     CDS             1141..1917
                     /locus_tag="VHM69_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742119.1"
                     /translation="MTGGLPDALETLLTTSLPGLFDGETVGLTVGAGLLEIDQEQSAD
                     ATASEPRPDDRVDDLGFDPEDPAGPYTLTQPPYPGPRRVWLVTGAADRLTLRSEEVVW
                     DRTDARVFSLALRPQRDVSAVTGIRVLYGVTAVYTKVKAVQTLDVRLTASDSDLLERA
                     ESLVVAVIQLNRQRLADEARAIYEDGDYGAAVELKNVGLVRGTTPEDDVRQLLMRAEL
                     ELKASRALGEDEGAPIERILTPGRPPDPDRPIDVHIDVEA"
     gene            1921..6948
                     /locus_tag="VHM69_16890"
     CDS             1921..6948
                     /locus_tag="VHM69_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2742120.1"
                     /translation="MSRTKAALILDPDRREEQLAYLSERVETLHEAGDQLWVSMTEDQ
                     AERLADQGIAVQLHEEADLIELPAARFDPAAGSPVPPEGLRAASPPEGTSSYHLVQFI
                     APPDPTWVHEVIRMGGNYIQTLPVNAGVFELTVLQATDVGQLGYVSWVGLYHPAYAVS
                     FRLAGRTEPFDAPSLRNLAVGPAGVPPGDGGNIRLRLFDDVSSEEIRPNVEGAATIVA
                     ETEYGFVVNADHSRITTLAGIPGVFAVEPFFQPTFKNNHAGAIMNTNQVRNVGNVNFL
                     VNLDGSGEIVGVIDSGLDTGVTPPGAPAIHADLGARVLQITNLAAPGTPARDNAAASG
                     PHGTHVTGSIAGDGTGSAGRVRGVAPNSHVIFHGPLGPNLMPGFRNAHDAGARVHSNS
                     WGTSPSFSCGGNSWPAPTNNRYVIGNTYVIDQFCFANPESLLLFASGNEENDIVPVGG
                     DGTPDMNFLTEEATSKNALTVGAVENVRSNDGDPTTHRASSAPPAAVGACPPIPLPYP
                     NPAWNALAGGAALGFTQSDNAGQLALFSNRGVVQASGGRIKPDLVAPGTNILSLRSTQ
                     QPAPPGVIPPHPSAANVGVSRTESALLYELMSGTSMATPQVSGAAILARQYYRARFGQ
                     MRRPLLLERLSALVDSPAIASHRDGRVAAWVQRDAGQNHLVAARYDRNWARQGGIIRL
                     QTNVGDHPAPVLARHGDNTFLVYRAGDNTIKLSLFDASLFLVNGFGIGGTVTLAPASR
                     SEDSRRPGICVQGNEVAVAWNETGGDKLLFRRFRTDNGAPIDSAPQNLGNMTSSSPHP
                     FIAHNGTRYTVVWAQTEGTDRKLRIRQVDSSGTPQGTQPGTLVTQTQDIRDPHLIWDG
                     RQGRFVVVWAGSDASPTGDIRCLFVDPLGSPSGAASTVSTVPAGNTARRPRISQHPEA
                     GYVLLWEDDTQNNRHDVYMAFLDNAGQPDRLPRDPTDPAILAPVARPSRQLLRISDTP
                     ENTAGFAGLVDADGVAVVWHSNDEINSDLLGVYGLSVTPLGAFQAQTDPNTPMIDSGR
                     YKNQTLLDHTLQTLTSVSMAWAGGSYYLLRRSPGNILDELHLIRTNADGLVDTTYGTR
                     SLDSGIFLSQHEMHWTGDRLVCASVGLVSDPVVYLLDASGEPVASFGTGGGQSITDSG
                     VTNPAISPQLGHTTQPSFRVIVAYGTGSPQPDIRYTVLNANGGTFVAPLTLARANGTA
                     RHGWFHYISSEARSIAAWHRDEAGTMKVFINRYSIGGAKQHASDIPLTTLPGQSMNPV
                     IAARPVDINSSQREYGLAWQYRASGTDPWEIRFSRLGRDGRPMNAAPAPPAPPGGTRD
                     VRVIFAAPSVWPPPGMPAGTDAIEPQLVSTYTHQNWNSARPWSPGYGLAWLGQPSGGN
                     RTLFFTALDENGVRSQLTLPPPYAAGAPRSEAAHIVQISTTGADVREFKLVWNGRTFR
                     LTWTETQGGRLRHMQTALTRTGNRAVYDLPSSSLVRATLINGATNINATPLPNIPTAP
                     ITAANRNQGYGWGRVNLRQSLSPSPPVTFHVRDDEAVGSGRRAQYRFYLPPDTRLLRV
                     TLAWTDPPRTDARIANNLHLRVVPPGGVGAGQEFHGNTWAAAPDSHLSRRVAPAAPFE
                     GVHNVEQVVVRDPASGIYDVEVVAEPFPANNFNQLRVQPFALVFVGSGQEVLFGPVAP
                     AGPIPVY"
     gene            7247..8335
                     /locus_tag="VHM69_16895"
     CDS             7247..8335
                     /locus_tag="VHM69_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEX2742121.1"
                     /translation="MSTFVGGLRLSGHFYEEAVRPVLETSFPGLQHSAALIGYGSEVL
                     GYDDARSTDHEWGPRLLLFLSEEDHRVRADSIVEILRNELPHEFGGYPTHFGEPDEEG
                     VRLAEKKASGPVDHKVEVHTVGGFFSAWTGLDPFEEWGSAEWLCVPQQRLLEVTAGRV
                     YHDGIGELQVARARLRYYPRDVWLYLLAAQWARVGEQEAFVGRTGEAGDELGSRLVAA
                     GLVRDIMGLCFLMERRYAPYAKWFGTAFSRLRCAPRLGPVLDAVLSARYWGEREGRLS
                     EAYGIVAGMHNALGLTPPLGTGVSRYHDRPYLVVHSGRFVAALERAIQDQAVLSVPHR
                     AGSIDQICDSTDVLSRPETYLKLKGFFE"
     gene            8916..9410
                     /locus_tag="VHM69_16900"
     CDS             8916..9410
                     /locus_tag="VHM69_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742122.1"
                     /translation="MLENPNFGATARAAEDLERGIVDQRLIATLRILAKEHRICVEAF
                     KQGHYFLPGVPDGPRIPKGYGKAGGLPNTHYYGRAADIRWVNGKPVEGHATDQDVLRL
                     GKILASIPLGRRPDQIIGPPRWSRALGYGRKEGWILAPDQLHLHKDHIHLGYTDAYSL
                     NNTR"
     gene            complement(9720..10670)
                     /locus_tag="VHM69_16905"
     CDS             complement(9720..10670)
                     /locus_tag="VHM69_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2742123.1"
                     /translation="MREIVRDTPAASLTAEERTGEERKGERSKATRRTELFLILAVLL
                     LGTNPVAVKYAVSTIAPLPFVALRFTVAGLLLLGAVRFLGSGGGVERKDLLAMMGVGA
                     VGVGMTNMLFTSGVNLTNASDTALLYAVVPVWGMILGSVLGLERPTLRGIFGVGIAFM
                     GIIVVVYGGLGGSGSSLEGNLLVLGATVCWGSYTVLSLPLLAKYSPLVVAAYTMFFGG
                     LATLPFALPGFPSVDWDTMSVGVWAAVAYSTVLVAAFGFFAWQRGASLLGANRVLVYQ
                     YLITFVGVISGIVLLGEELGFEKIIGGSIILFGVYLARRQ"
     gene            10798..11412
                     /locus_tag="VHM69_16910"
     CDS             10798..11412
                     /locus_tag="VHM69_16910"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2742124.1"
                     /translation="MKHAADKYPDARPPGRPRSERARRAILQAANELLESEGFAAVTV
                     EAIAERAGVSKATVYRWWPNRAAVVMDGFLSTVSSEVPFPHTGHAREDIQIHMRRLAE
                     AFRGKIGRTVAALIAEGQADPELADALRSRWLSVRRAEAREILELGIERGELRENLDP
                     EVAVDVLYGPIYYRMLVGHAPLDDDFTDALADHVFSGLGARDPA"
     gene            11588..12013
                     /locus_tag="VHM69_16915"
     CDS             11588..12013
                     /locus_tag="VHM69_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742125.1"
                     /translation="MWAAAAHVLALTVILAQPVASLAWLGGTHATPEQAALHEAAVEH
                     GHYYHHGAPRHDVHYPGDGKTDTQFSQVKAGPEFTTAASHAAPFQDLLRATLAGAPKA
                     PMPDDLLRYAPPAGGAPSQYSPPVPHRPPILLFSTFAFM"
     gene            12086..12466
                     /locus_tag="VHM69_16920"
     CDS             12086..12466
                     /locus_tag="VHM69_16920"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEX2742126.1"
                     /translation="MKRLLFVGAAALSLYAMLAVASPVWAHVVVSPEEVKAGDYATLT
                     VSVPTEKELPTTQIRVEVPEGFLLSGVQPVPGWEHTFEDDGGVVTAVTFSGGEIHPRE
                     FQQFLVQAQAPDEPGEYPWKAIQT"
     gene            12479..12721
                     /locus_tag="VHM69_16925"
     CDS             12479..12721
                     /locus_tag="VHM69_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX2742127.1"
                     /translation="MVKWTGPPDAEEPASVVEVVSAGSGGHESSPETQEGGASQEASA
                     EAEVLADTGGTNLAVYAGIGVVGLAISALLARRLLG"
     gene            12724..13299
                     /locus_tag="VHM69_16930"
     CDS             12724..13299
                     /locus_tag="VHM69_16930"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX2742128.1"
                     /translation="MAPVTGIGRLPIALWLSVAWVLLSSVPALAHANLVGSFPSAGLK
                     SSTPPEQVRLRFNEPVDAEFDPVVVRDSEGDRVDEGDARVDPEDARVVLADLEKLPEG
                     SYTVEWRVTSIDGHVVEGRYGFAVTGGSGDERPNAEERAGQADREPATQGGRPDGSVP
                     ILTYSALSLGLVAAVVAAALLLTWLVRRRRP"
     gene            13296..14330
                     /locus_tag="VHM69_16935"
     CDS             13296..14330
                     /locus_tag="VHM69_16935"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX2742129.1"
                     /translation="MSVRTHIAGATSVRSSAIVVASVAALLAAAYLGDPAYAQGIHGG
                     RSAAPHDQENTVAIEHGVTLAVTAFLAGLAPFAALVWLPASREVGAGRDALGPFVLLA
                     WVLLWCLTVAGVGELAAYATRASGEPISTVLLWQALSDTRVGAVWLARLGFGLVTTAA
                     ITAAVRAGWDWLWWTASGTGSLLLMTLTQLSHAAATGRFLPFLADWTHAMAATIWMGG
                     LLGFVVVLFAGPLDAVPADRGARLRERSARRFSTVTTISVVVLAATGLYASVLHVPSP
                     RALVETPYWIALTIKLGLLVLVLTVGAANFLLRGRGPFGRLVVVELILALALFVATGF
                     LTSLPPASSA"
     gene            complement(14738..15085)
                     /locus_tag="VHM69_16940"
     CDS             complement(14738..15085)
                     /locus_tag="VHM69_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742130.1"
                     /translation="MSEVHEPQKFSPSDTISIDMEFQNESGVDNVYALFVNTEYLKNT
                     IGLYGNGEGSTKLSIRMQSRAASYPMPGEYLCQYIHVTDIHGNYAVSYPDRKVCFWVD
                     GSTDAYEAHDPGS"
     gene            16239..>16892
                     /locus_tag="VHM69_16945"
     CDS             16239..>16892
                     /locus_tag="VHM69_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742131.1"
                     /translation="MRIEEDREHNVEEVVSQEVLPSMIKWWQQRSLFTRLLAYAVAAT
                     LAFVMAASVGAIAALVVSGNLSWPTGEKPRPEGVSPAGEQGDTPQRQQSDADRPKQER
                     AGATKGDQAASQQAASQQVETTYVHEVGEIQANSVETFLDSHNKLLRYDALTTDDVEE
                     LQANQAALKASTHQASELNAPRKYSEQNETFLSAINELQEAVQLAYTLAADPTSATQS
                     "
BASE COUNT         3221 a         5352 c         5322 g         2997 t
ORIGIN      
        1 ctgcaagcga agaacaatga tctcgaaggc aggataatgg acctcgagtt gagcgctttt
       61 gctaaggatc gggaggccga cgccaccgaa gcagccgagc gtggagccgg caactcggcg
      121 aacggctctc cgaagtgatg ggcaagggag ccggctcgta ctggtcaaag atgtagaggt
      181 ggccgcgata gcctggcggc gatttttgga atagatgggt agctgatggg cgagagtgcc
      241 gttagagtac gacgaaaaga tacgtcgcat cttcaaggag cggaccgagt cgtgaccttt
      301 cccaaagtcg tcctggccga gtacgatcca gggtggccgg cgctcttcga agccgagaag
      361 aagaggatac tggacgctat cggcctacgc gtcctggcga tcgaacacgt cggcagcacg
      421 gcggtaccgg gaatgatggc caagcccatc atcgacgtga tggtcggcac caggaacgtc
      481 gctgctacgg cggacgcttg caccgccccg ctacagaaca tcgggtacgc gtccgtcccg
      541 ctgccggcga ttatggacag gcgctttttt aagaagggtg cctggcttct cgagtgtatg
      601 taccacctgc acatggtcga gttgggtggg gaaacctggc acagatatac gctattccgt
      661 gacgtactgc gcgcgtaccc ggacgtggcg cgaagctacc aggagctgaa gcaacaactc
      721 gccgtcgagt acaggtttga ccgggccgcc tacaccgcgg ccaagaattg gtttgtcgaa
      781 ggcgtaatcg ccagggccga aaacgagttc aaatcatgac gaggaaagtc gacgcgatcc
      841 ggcgatgcct cagagttgta actgtatcta tgcggcggcc tagagaaagg tggatctatg
      901 cgagagcgac tccagaagag actcgctgag ttgaaaaccg agttcgaaag tggtcagcag
      961 acactcgatc aacttgagac gcaagccgga acggtgcggc agaccttgct gcgcatcagc
     1021 ggggcaatcc aggtgcttga ggaagagctg ggcgacactg gccagcccca tgggaacagc
     1081 tcggaggacg aaacgttgac cgggtctgcg accgcgagca atcgaagcta ggggcgcagg
     1141 atgaccggcg gtctgcccga cgctctggag actctgctca ccaccagcct gcccggcctg
     1201 ttcgacggtg agacggttgg cctgaccgta ggcgctggtc tgctggagat agatcaggag
     1261 cagtccgccg acgcgacagc gagcgagccg cgaccggacg accgcgtgga cgacctgggg
     1321 ttcgaccctg aggacccggc gggcccgtat acgctcaccc agccgccgta tcctgggccg
     1381 cgccgcgtgt ggctggtcac gggagccgcc gaccggctca cattgaggtc ggaagaagtg
     1441 gtctgggacc ggacggatgc cagggtcttc tccctggcgt tacggccgca gcgcgacgtg
     1501 tccgccgtta cgggaatccg cgtgctctac ggcgtgacgg ccgtctacac gaaggtcaag
     1561 gccgtacaga ccctcgacgt gcggctgact gcctcggatt cggacctgct ggagagggcg
     1621 gagtcgctgg tggtcgccgt gatccagcta aaccgccaga gactcgccga cgaagcgcgg
     1681 gccatctacg aggacgggga ttacggcgcc gccgtcgaat tgaagaatgt tggcctggta
     1741 aggggaacca cgcccgagga cgatgtacgc cagctcctca tgcgtgcgga attggagctt
     1801 aaggcgagtc gcgcgctcgg ggaggacgag ggcgccccga tcgagcgcat actgacgccg
     1861 ggaaggcctc ccgaccccga caggccgatc gacgtccaca tcgacgtgga ggcgtagatt
     1921 atgagtcgta ctaaggccgc cctgatactc gacccagacc ggcgcgagga gcaactcgcc
     1981 tacctctctg agagggtcga gaccctccac gaggccggag accagctgtg ggtctcgatg
     2041 accgaggacc aggcagagcg gctcgccgac cagggcatcg cggtgcagtt gcacgaggag
     2101 gctgatctga tcgagcttcc cgccgcaagg ttcgaccccg ccgcagggag ccctgtacct
     2161 cccgagggcc tgcgtgccgc atcgccaccc gagggcacgt ccagctacca cctcgtgcag
     2221 ttcatagcgc ccccagatcc cacctgggtc cacgaggtca tccggatggg cggaaactac
     2281 atccagaccc tgccggtcaa cgcgggcgtc ttcgaactga ccgtgctcca ggcgacggac
     2341 gtcgggcagc ttgggtacgt gagttgggtc ggcctctacc acccggcgta cgcggtcagc
     2401 ttcaggctgg caggcagaac ggagccgttc gacgccccgt cattgcggaa cctcgccgtc
     2461 ggtccggccg gcgtgccacc cggcgacggc gggaatatca ggctgcgcct gttcgacgac
     2521 gtctcctcag aagagatcag gccgaacgtg gaaggcgcgg cgacgatagt cgcggagacc
     2581 gagtacgggt tcgtcgtgaa cgcggatcac tcccggataa cgaccctcgc ggggatacca
     2641 ggcgtcttcg cggtggagcc gttcttccaa cccacgttca agaacaacca cgccggcgcc
     2701 ataatgaaca ccaaccaggt caggaacgtg ggtaacgtga atttcctggt aaacctcgac
     2761 gggtctgggg agatcgttgg cgtgatcgac agcggcctgg acacgggcgt tacccctccg
     2821 ggagcgccgg cgatacacgc tgacctcggc gccagggtgc tccagataac caaccttgcg
     2881 gcgccgggca cccccgcccg agacaacgcc gcggccagcg gaccgcacgg gacgcacgtc
     2941 accggatcga tcgccggtga cgggacgggg tcggcggggc gggtacgtgg cgtcgccccg
     3001 aactcccacg tcatctttca cggaccgttg ggccccaacc tgatgcctgg tttccgcaac
     3061 gcccacgacg cgggcgccag ggttcacagc aactcctggg ggacgtcacc gtccttttcc
     3121 tgcggcggga attcgtggcc ggcgcccacc aacaaccggt acgtcatcgg caacacgtac
     3181 gtgatcgacc agttctgctt cgcgaacccc gagagcctgt tgctgttcgc atccggcaac
     3241 gaggagaacg acatcgtacc cgtgggcggc gacggcacgc ccgacatgaa cttcctcacc
     3301 gaggaggcga cctcgaagaa cgccctcacg gtgggagccg tcgagaacgt gaggagcaac
     3361 gacggcgacc caacgaccca ccgtgcttct tccgcgcctc ccgccgcggt aggcgcttgc
     3421 ccgcccatac ctctccctta ccccaacccg gcgtggaacg cgctggctgg cggggcggcg
     3481 ctggggttca cgcagtcgga caacgccggc cagctcgcgc tgttcagcaa ccggggggtg
     3541 gtgcaggcct cgggaggcag gataaagccg gatctggtgg cgccgggaac gaacatactg
     3601 tccttgcgct cgacgcaaca gccggcgccg cctggggtga ttccgcccca tccgagtgcg
     3661 gccaacgttg gggtctcgcg taccgagagt gccctgctct atgagctgat gagcggcacg
     3721 agcatggcca caccccaggt ctcgggcgcg gcgatactgg cccgccagta ctatcgcgcc
     3781 cgcttcgggc agatgaggcg gccactgctc ctcgaacggc tgagcgcact cgtggattcg
     3841 ccggccatcg cctcacaccg cgacgggcgc gtggccgcct gggtccagcg cgacgccgga
     3901 cagaaccacc tggtcgcggc gcgctacgac cgcaactggg cgcgtcaggg cggtatcatt
     3961 cggctacaga cgaacgtggg agatcacccc gcgcccgtgc tcgcccggca cggcgacaac
     4021 acgttcctcg tgtaccgggc tggcgacaac accattaagt tgagcctctt cgacgcctcg
     4081 ttgtttctcg tgaacggttt cgggatcggc ggcacggtca ccctcgcgcc ggcctcccgc
     4141 tccgaggact cgcgccggcc cggcatctgc gtccagggca acgaggtcgc cgtggcatgg
     4201 aacgagaccg gcggcgacaa gttgttgttt cgacgattca gaacggataa cggcgccccc
     4261 atagactccg ccccgcagaa cctggggaac atgaccagtt cctcgccgca cccgttcatc
     4321 gcgcacaacg ggacgcgtta caccgtcgtg tgggcccaga cagaagggac ggatcgcaag
     4381 ctcagaatac gccaggtgga tagctcgggg acgccccagg gcacgcagcc ggggacgctg
     4441 gtgacgcaga cgcaggacat ccgtgacccc catctcatct gggacggacg ccagggtcgc
     4501 ttcgtggtcg tatgggccgg ctccgacgcc agccccaccg gcgacatccg ctgcctgttc
     4561 gtcgatcccc tcggctcccc ttcaggcgct gcgagcacgg tgagcacggt gcccgccggc
     4621 aacacggcgc gacggccccg catctcgcag cacccggaag ccggttacgt cctgttgtgg
     4681 gaagacgaca cccagaacaa ccgccacgac gtctacatgg ccttcctcga caacgcgggt
     4741 cagcctgacc gcctcccgcg cgacccgacc gacccagcca ttctggcacc cgtcgcgcgg
     4801 ccctcaaggc agctactgcg catatccgat accccggaga acacggcggg gttcgccggt
     4861 ctggtcgacg cggacggcgt cgcggtagtg tggcacagca acgacgagat caactcggat
     4921 ctgctcggtg tttacgggtt gagcgtaacg cccctcggtg ccttccaggc gcagacggat
     4981 cccaacacgc ccatgatcga cagcggacgt tacaagaacc agacactgct cgatcacacg
     5041 cttcagaccc tcacgagcgt ctcgatggcc tgggcgggag ggagctatta cctgctgcgc
     5101 aggagccctg ggaacatact cgacgagctg cacctaatcc ggaccaacgc tgacgggctg
     5161 gtagacacca cctacggcac ccgctcgctc gacagcggga tctttctcag ccagcacgag
     5221 atgcactgga ccggggaccg gctggtctgc gcttcggtgg gcttggtctc cgatccagtc
     5281 gtctacctgc ttgacgcctc gggcgagccc gtggcgtcgt tcggtactgg cggcggtcaa
     5341 tccatcactg acagcggcgt aacgaacccc gcgatcagcc ctcagctggg tcacaccacc
     5401 cagcccagct tcagggtcat cgtggcatac gggacgggtt cgcctcagcc agatatccgc
     5461 tacactgttc tcaacgccaa cgggggtact ttcgtcgccc ccctcaccct cgctcgcgcg
     5521 aacggcaccg cacggcacgg ctggttccac tacatcagca gcgaagctcg ctcgatcgcg
     5581 gcgtggcaca gggatgaggc cggcaccatg aaggtgttca tcaaccggta ctcgatcggg
     5641 ggcgcgaagc agcacgccag cgacatcccg ctaaccacgc tccccgggca atcgatgaac
     5701 ccggtgatcg ccgcccgccc ggtggacatc aacagcagcc agcgggaata cgggctcgcg
     5761 tggcagtacc gcgccagcgg cacagatccc tgggagatcc gcttcagccg cctgggccga
     5821 gatggccggc ccatgaacgc cgcgcccgcg ccgcccgcgc cgcctggtgg cacgcgggac
     5881 gtgcgcgtta tcttcgccgc cccatcggtg tggccgccgc caggtatgcc ggcgggtaca
     5941 gacgcgatcg agcctcaact cgtctccacc tacacgcacc agaactggaa cagcgcgcgc
     6001 ccgtggagcc ccggctacgg gctggcctgg ttggggcaac cctccggcgg caaccggacg
     6061 ctattcttta cggcgctgga cgaaaacggc gtcaggtcgc agctgaccct gccaccgccg
     6121 tacgcggccg gggcgccccg cagcgaggcc gcccacatcg tgcagatcag caccacgggc
     6181 gccgacgttc gggagttcaa gttggtctgg aacgggcgta ccttccgctt gacgtggacg
     6241 gagacccagg ggggacggct ccggcacatg cagaccgcgc tcacccggac cgggaaccgg
     6301 gccgtctatg atctgccgag ctcctcgctg gtgcgcgcca cgctcatcaa cggagccacc
     6361 aacatcaacg ccacgccact acccaacatt cccaccgcgc cgatcacagc cgcaaaccgg
     6421 aaccaggggt acggatgggg gcgggtgaac ctccgccagt ccttgtctcc ctctcccccc
     6481 gtgactttcc acgtgcggga cgacgaggcc gtggggtcgg ggcgccgcgc gcagtaccgg
     6541 ttctacctgc cgccggacac caggctgctg cgggtgaccc tggcgtggac ggatcctcct
     6601 cgcaccgacg ccaggatagc gaacaacctg cacctgaggg tcgttcctcc gggcggcgtg
     6661 ggggctggcc aggagttcca cggcaacacg tgggcggcgg cgccggattc tcacctgagc
     6721 cgccgcgtgg cccccgccgc gccctttgaa ggcgtgcaca acgtcgagca ggtcgtcgtt
     6781 cgtgatccgg ccagcggtat ctacgacgtg gaggtcgtcg ctgagccttt ccccgctaac
     6841 aacttcaacc agcttcgcgt gcagccgttc gcactcgtct tcgtgggaag cggacaagag
     6901 gtcctcttcg ggcctgtggc gcccgctggt ccgatcccgg tgtactgagc gccctcgtcg
     6961 cgcgaacggc tgacaaagac acgggcaacg accgtcatgt cagaagttcc ataagattat
     7021 cccctgagcc tttagagagc gaggccggcg cagaaggaac ccgtgccttc aactccagcg
     7081 tactccagat agccggactc actcgcctga ggtcgcggta cctgcgactt cgccgggatc
     7141 ctcgtggcca acccaatgat tgtggtccct cagttgctcg ctccggtatg tctccccggg
     7201 acataatgta tcgaatcggg cagacggacg gttgaggagg agcttggtgt ctacgttcgt
     7261 gggcggactg cgtttgagcg gccacttcta cgaggaggcg gtaaggccgg tcctcgaaac
     7321 ctccttcccc gggcttcaac attcagcagc cctcatcggc tatggctccg aggtattggg
     7381 ctacgacgac gcccgttcga ccgaccacga gtgggggccg cgactcctgc tattcctctc
     7441 cgaggaggac caccgcgtac gggccgatag catcgtcgag atcctccgca acgagctgcc
     7501 tcacgagttc ggggggtacc caacccactt cggagaaccg gacgaggagg gggttcggct
     7561 cgccgagaag aaggcgtccg gacccgtgga ccacaaggtc gaggtgcata cggtcggggg
     7621 cttcttctcc gcgtggacgg gtttagatcc tttcgaggag tgggggtcgg cagagtggct
     7681 gtgcgtgccg cagcagaggc tcctggaggt caccgccggc cgcgtctacc atgatggtat
     7741 cggagaacta caggtggcgc gcgccaggct tcgctactac ccccgtgacg tgtggctgta
     7801 cctgctcgcg gcgcagtggg cgcgcgtggg ggagcaggag gcgttcgtcg ggaggacggg
     7861 cgaggcgggt gacgagctgg gttcccgcct ggtcgcggcc ggattggtgc gcgacattat
     7921 ggggttgtgc ttcctcatgg agaggcgcta cgccccttac gccaaatggt tcggcacggc
     7981 cttctcccgg ttgcggtgcg cgccacgtct cggccccgtc ttggacgcgg tgctaagcgc
     8041 gcggtactgg ggggagcggg aaggccgcct ctccgaggcc tacggcatcg tggccgggat
     8101 gcacaacgcc ctcggcctaa cgccgccgct tggcaccggg gtctcccgct accacgaccg
     8161 gccctacctc gtcgtccact ccgggcgctt tgtggcggcc ctcgaaaggg ccatacagga
     8221 ccaggcggtc ctctccgtgc cgcacagagc gggctctatc gaccagatct gcgacagcac
     8281 cgacgtgctc tcacgacccg agacttacct caagctcaag ggattcttcg agtaagcaac
     8341 cgacgaaacg gctccaaccc cttcaccaag agttcctgac ggccccggca gggcaccgtc
     8401 gcgtgttctt cccaggcaag cgctacagcg accgcaacga gcatcggacc gacaaaccac
     8461 cccgcgggca gaccaatgcc tcgggcgagc agtcccgcca atgaatagcc gataacacta
     8521 ctcagctcct tcaagccgac gcgccgctga tccaccggtc gatttcggtt ctctcagcgc
     8581 taaaggttcg agcgtcgtcc attatcgtcg ctgtcagcaa ggtccctggg tatataagct
     8641 agccgatgtc gaccactcga aacacctgca agacgtagct acttcccacg tcataatatg
     8701 atgaggtgga acaggactgt atcctactgt ggtcccggcg aacgatcgca agtgcggcca
     8761 tgcttctgag tgtcgctcta ctgctcacca cttgtgggag cttgaaggat gtggacctca
     8821 ctccgggatg cctaccgggc gtcggccggg gaatgtggga tccaggttca ctgaaaggtg
     8881 gtgacgctac agcgaatagc aatgaggtgc gaaagttgct ggagaatccc aacttcgggg
     8941 caactgcgcg ggcggcagaa gatctggagc gaggaattgt ggaccagcgc ctcatagcca
     9001 cgctccgtat cctggccaaa gagcatcgga tctgcgtcga agcattcaaa cagggtcatt
     9061 acttcctgcc aggcgtcccg gatggtccgc gcatacccaa gggctacgga aaggctggtg
     9121 gcctacccaa tacccattac tacggcaggg ccgctgacat ccggtgggtg aacggcaagc
     9181 ctgtcgaagg ccacgctaca gaccaggacg tcttaaggtt gggtaaaatc cttgccagca
     9241 tacctcttgg gaggagacct gatcagataa ttgggccgcc aagatggtcc agggctctgg
     9301 gatacgggcg caaggagggc tggattctgg cccccgacca gctccacctg cacaaagatc
     9361 acatccacct tggctatacg gacgcataca gcctcaacaa tactcggtga acaccttagt
     9421 actagataac gtacccctta cagcggcttg cggaaggatt gatcctccac aagccaggct
     9481 acaggtatca ggtttcaggc atcaggaagg tcttgccgga cggtggtcgc taaggtgcac
     9541 ctctggggcg aagtggatca atacttatgt actgcgctgt tagagcttcc accgacgtct
     9601 ttgtagcggt gtggaacggg tcctcagtgt tcactatgct tgagtaaggc aggccgtaga
     9661 ggtcgctgcg aaccgctctc aggttcattc cgccgcgccg aagggaggag agacactcgc
     9721 tactgacgac gggccaggta cacgccaaag aggatgatcg accctccgat gatcttctcg
     9781 aaccctagct cctcgcccaa gagcacgatg cccgagatga ccccgacgaa tgtgatgaga
     9841 tactggtaaa cgagcactct gttggcccca aggagggaag cccccctctg ccaggcgaag
     9901 aacccgaatg cggccaccag gacggtcgag taggcgaccg ccgcccacac cccgacgctc
     9961 atcgtatccc agtccacgct cggaaagcct ggcaaggcga acggtaaggt ggccaggcct
    10021 ccaaagaaca tggtataggc agcgaccacc agcggcgagt acttcgcaag gaggggtaag
    10081 gagagaacgg tgtacgaccc ccagcacacc gtggctccca ataccaggag atttccttcg
    10141 agactcgagc cgcttccgcc caaacccccg tacacaacga cgatgatgcc cataaaggct
    10201 atccccaccc cgaagatgcc cctcagggtg ggcctttcta atcccaacac ggagcccagg
    10261 atcatgcccc atacaggcac gacagcgtat agcaaggcag tgtccgaggc gttcgtgagg
    10321 ttcacgcccg aagtgaagag catgttcgtc atcccgacgc ctacagcacc tacccccatc
    10381 atggccagta ggtccttcct ctcgacacct cctccggatc ccaagaaacg tacggcgcca
    10441 aggagcagca gacccgccac tgtaaacctc agggctacga agggcagggg ggcaatggtg
    10501 gagacggcgt acttgacggc cacggggttg gtgcccaaga ggaggaccgc caggatcagg
    10561 aagagttccg tacgccgggt cgccttcgac cgttcacctt tgcgttcttc cccggttcgt
    10621 tcctcagcgg tgagcgacgc ggcaggcgtg tcccgtacta tctccctcac ggtctgtcgg
    10681 ctttcgtcat ttgccctgtg cgcctcattc gctttgtggt ctttcgatac acaataacaa
    10741 aacgaaacgt tttgtctagt aattcccttc actccgcact gctgctagta tgcgaatatg
    10801 aagcatgctg cggacaaata ccctgatgcc cgaccgcctg gccgacctcg cagtgagaga
    10861 gcacggcgtg cgatcctaca agcggccaac gaactgctcg agagcgaggg gttcgccgcg
    10921 gtaacggtgg aggcgatcgc cgagcgggca ggggtctcca aggccacggt ataccgctgg
    10981 tggcccaaca gggccgcggt ggtgatggac ggcttcctct cgaccgtctc ctccgaggtc
    11041 ccattcccac acacgggtca cgcgcgggag gacatccaga tccacatgcg caggctcgca
    11101 gaagcgttca ggggtaagat agggcgaacg gtggctgcct tgatagccga gggccaggca
    11161 gacccggagc tcgccgatgc tttacgttcg cgctggcttt cggtaaggcg ggcggaagcc
    11221 agggagatac tcgagcttgg catcgagcgc ggcgagctca gagagaacct cgacccggag
    11281 gtggccgtcg acgtcctcta cgggcccatt tactaccgga tgcttgtcgg ccacgccccg
    11341 ctcgatgacg atttcaccga cgcccttgcc gaccacgtct tctccggcct gggagcgcga
    11401 gatccagcgt agcccgggac gcgggtgcat ggtcggtcct cgctcgtagc gcggcttatg
    11461 agaattggcc aggtcgagta tccaaagact gagaatacgg attctcagaa ggtcggcgaa
    11521 tggcgtaaga tggccgaagg ttgctagcct ggccgtccca taagcaccta ctctcgaatg
    11581 cccggcgttg tgggcagcgg ccgcgcacgt cctggcgctg accgtgatcc tcgcccagcc
    11641 cgtcgcgtct ctggcatggt taggtggaac gcatgcgact ccagagcagg ccgccctcca
    11701 cgaggctgcc gtcgagcatg ggcactacta tcatcatggc gcacccaggc acgacgtaca
    11761 ctacccaggg gacggcaaga cggatactca gttctctcag gtcaaggccg ggccggagtt
    11821 caccactgcc gcgtctcatg cggcaccatt ccaagacctc ctcagggcga cactagccgg
    11881 agcccccaaa gcacccatgc ccgacgacct cctgcgctac gctcctcccg cggggggcgc
    11941 tccatcccag tattcgcccc ccgttccgca caggcctccg atcctccttt tctcgacctt
    12001 cgccttcatg taggagtaga ggcccaacct gggccggaca gtactgcgtt tgtccggccg
    12061 agcgagggcg tgtttggagg tgcctatgaa gcgattattg ttcgttgggg ctgcggccct
    12121 tagcctctac gcgatgctcg ccgtagcatc cccggtctgg gcccacgtgg tcgtctcccc
    12181 ggaggaggtg aaggctggtg actacgcgac actaaccgtc tccgtgccca ccgagaagga
    12241 gttgccaacc acgcagatac gggtggaagt gccggaaggg ttcttgctct ccggggtgca
    12301 gcccgtgccc ggttgggagc acaccttcga ggatgacggc ggcgtcgtga cggccgtgac
    12361 tttctccggg ggggagatcc atccgcggga gttccaacag tttctcgtcc aggcccaggc
    12421 ccccgacgag ccgggtgagt acccctggaa ggcgattcag acctaagagg acggaagcgt
    12481 ggtgaagtgg acaggtcctc cagatgccga agaacccgcc tccgtggtag aggttgtctc
    12541 cgccgggtcc gggggccatg agtctagccc ggagacccag gagggcggcg cctcccagga
    12601 agcaagtgct gaagcagagg tcctggccga cacgggcggg acgaacctgg ccgtctacgc
    12661 cggcatcggc gtggtcggtc tggcgatttc ggccctgttg gcgcggcggc tcctgggata
    12721 gtggtggcgc ctgtaacggg catagggcga ttgccaatcg ccctttggtt gtccgtcgcc
    12781 tgggtgttgc tgtccagcgt cccggctctg gcccacgcca accttgttgg gtctttcccc
    12841 tcggcggggc tcaagtcgtc cacgcccccc gagcaggtgc ggctacggtt caacgaaccg
    12901 gtggacgcgg agttcgaccc ggtggtggtg cgcgattccg aaggcgaccg cgtggacgaa
    12961 ggcgacgcac gcgtggaccc ggaggatgca agggtggtgt tggcggacct cgagaagctg
    13021 cccgaaggtt cctacaccgt ggagtggcgt gtaacctcca tagacggaca cgtcgtggaa
    13081 gggcggtacg gcttcgcggt caccggcggg tcgggggacg agagaccgaa cgccgaggag
    13141 cgcgcggggc aggccgatag agaaccggcg acgcaaggcg gcaggccgga tggctccgtg
    13201 cccatcctta cctacagcgc cctgagcctg gggctggtgg ccgccgtcgt ggcggcggcg
    13261 ctattgctga cctggctggt gcgtcggcgc aggccgtgag tgtccgcacg cacatcgccg
    13321 gggcaacgag cgtgcggtcc tccgccatcg ttgtggcatc cgtggccgca ttattagcgg
    13381 cggcatacct tggggacccc gcctatgcgc aaggcatcca cggtgggcgc tcggcggcgc
    13441 cccacgatca agagaacacg gtcgccattg aacacggggt gacccttgcc gttacggcct
    13501 tccttgccgg gctggcaccg ttcgctgctc tcgtatggct cccggcaagc agggaggtcg
    13561 gcgccggccg ggacgcgcta gggcccttcg ttctcctggc ttgggtgctg ctctggtgcc
    13621 tcactgtcgc gggcgtgggc gagttggccg cgtacgcgac gcgagcctcc ggtgagccca
    13681 tcagcaccgt gctgttgtgg caggcgcttt ccgacacgag ggtcggcgcc gtctggctcg
    13741 cccggctcgg cttcggactc gttacgaccg ccgcgatcac cgcggcagtg cgggcaggct
    13801 gggactggct ttggtggacg gcgagcggga caggaagcct gctcctgatg actttgaccc
    13861 agctcagcca cgccgccgcc acgggccgct tcttgccctt cctggctgac tggacgcacg
    13921 ctatggcggc gacaatctgg atgggcggcc tgctgggctt cgtcgtcgtg ctctttgccg
    13981 gtcctctgga cgccgtgccc gcggaccgtg gagcgaggct gcgcgagcgc tcggcgcgcc
    14041 gcttctcgac ggtgaccacc atctctgtcg tggtcctcgc tgccacgggc ctctacgcca
    14101 gcgtgctgca cgtgccgagc ccgcgggcgt tggtcgagac cccgtactgg attgccctca
    14161 ccatcaagct cgggctactc gtactcgtgc tcaccgtagg cgcggcgaac ttcctgctgc
    14221 gcggacgcgg gccgttcggt cgcctggtcg tcgtcgagct gatcctggca ctcgcactgt
    14281 tcgttgccac gggctttctg accagcctgc ctcctgcgag ctccgcgtag cacgcacagg
    14341 ctcgcgctcg gcaggactcg aacctgcgac cttctgattc gtagcgagac gttccgcaca
    14401 tcagtagagt cccgtctggt ctcgttttgg ccctgttgat cccgatgaat cccggcggac
    14461 acccacccat aagtggtaga ggagagatca catcccgctg aaccttacta atgtagggtt
    14521 gggctaacta ctcgttgggt gagaggtagg tgttcaagaa gcgaagactg ttgaagatga
    14581 cggggtaaat gcgttcttgg aaggttgcat agtcccgacc gccacctaag gttctcatgc
    14641 ggaccctcat aatgatcata cgataatgcc tctgacgcag tccgagaacg aggacctact
    14701 gatacgctac attgattggt cagggcgggc ctgctatcta actgccagga tcgtgagctt
    14761 cgtaagcgtc ggtgcttcca tccacccaga agcagacctt ccgatcaggg taggaaaccg
    14821 cgtagttgcc gtgtatatca gtgacatgaa tgtactgaca caggtactct ccgggcattg
    14881 gatagcttgc ggctcgtgac tgcatcctaa tactgagctt ggtgcttcct tcgccgttgc
    14941 cgtacaggcc tatggtgttc ttgagatact cagtgttgac aaagagcgcg tagacgttat
    15001 ccacgccact ctcgttttgg aactccatgt cgatgcttat ggtgtcgctt ggcgaaaact
    15061 tttgcggctc gtgtacttcc gacatagtgc gttgtctcct ccgtctgtct agcagggatc
    15121 gcgagggcag tctcgccgcc ccttcttgct atacgacgag gtcaacatac gcctccgtag
    15181 ccttgaaacg catcccccgg atgacctatt tccataagac ctggcccacc cgcgtactgg
    15241 ctcattcacc caaccgcgtg gagaactgtt tctcagaagt ttgcgcagta gtggatttat
    15301 ggaacgcgtg cccggtcggc agcgcaatcc tactcttgtg gtcgtttcta gctgcccgtt
    15361 cgatgtcctc cctgctagcg acccacaccc cgaagcgggc cactgcagtt ccatcaggcc
    15421 tccttagcac ccaggaggct gcgtctgagg tatccaatcg gtagcccggg ggaagatgtt
    15481 cgcctttgat ttcccaacct ctcatctaaa ccatactcta cacacaatgg gagaacggtc
    15541 gcatctgcca gatagcctat tccaagtgca ctatgtgccc gacactctcg actgaagtgc
    15601 gtcagggcct tgacccctac agctctcctc aggaggtcat agcgcagccg aatagcaaac
    15661 aacggcaact atggtgcaaa gcagaatgtg gccactcgtg cgaacccaca tggggggctc
    15721 cctatacaag cgaggctcat tcgcaatatc ctcgagggct agcctgcacg atatgtggtg
    15781 gaattctttg gcctgcgagt gacttcttgt catagacgtt gtctccttct tccctggagc
    15841 ggataagagt gcgaaagcct gctccagagt tgcctaacga gtcgatcata gcctgcgtaa
    15901 gagcagcgcg cataggccgg gtggctcatc ctcaagtaga ttttctgaag gctcatggaa
    15961 gtacatgctc agaagagcag atgcgggagt atcagtgaag tttccgctgg gttaccaggc
    16021 cgagcggcct tgccgcatgc tcgcttttgg cacaatcaag ctagtgcgct gcggatcgtt
    16081 tctgagttgc gtcaagtgct gtatgtggct atgatgctac cactacaaaa ctaggttggt
    16141 agaggcgaag attaccgctc caagcctggg cacccggagc ggggggagtc agtggtgcaa
    16201 ccggctacct gaggtcgtag tgaccttggt ggcctgtttt gcgtattgag gaggatcgag
    16261 aacacaatgt cgaagaagta gtgtcgcaag aggtcctacc gagcatgatc aagtggtggc
    16321 agcagcgctc gttgttcacg agattactgg catacgccgt agcagcaact ttggcgttcg
    16381 tcatggctgc gagcgtgggt gcgattgcgg ccctcgtggt gagcggcaat ctaagctggc
    16441 ccacaggaga aaagccaaga ccagaagggg tcagcccggc gggtgagcaa ggcgatactc
    16501 ctcaacgtca acaatcagac gctgatcggc cgaaacaaga aagggctggt gccaccaagg
    16561 gagatcaagc cgcatctcag caagccgcat ctcagcaagt cgagactacg tatgttcatg
    16621 aggttggcga aatacaagcc aattctgtgg agacgttctt ggatagccat aacaagctac
    16681 tgcgttacga tgctctcacc accgatgatg tcgaggagct acaagctaac caggctgccc
    16741 tcaaggcatc tactcatcag gccagcgagc tcaatgcacc tcggaagtat agtgagcaga
    16801 acgagacatt tctttctgca atcaatgagc tgcaggaagc cgtccaatta gcgtacacct
    16861 tggccgccga tcccacctct gcaacccagt cc
//
LOCUS       DATAIR010000712         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_212900, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000712 DATAIR010000000
VERSION     DATAIR010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212900"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..757
                     /gene="ssrA"
                     /locus_tag="VHM69_16950"
     tmRNA           403..757
                     /gene="ssrA"
                     /locus_tag="VHM69_16950"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            891..2384
                     /locus_tag="VHM69_16955"
     CDS             891..2384
                     /locus_tag="VHM69_16955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2742132.1"
                     /translation="MSPVPGPVDRESFFEAQRRHRRTTWRLSAVCVAAVAVMGIPLSL
                     VLTPVLYTLVLVFAHLADLVAPVPESFWQTLAAGASLMIRVIGGFLGEEAPASVTQTL
                     VGLSVMLLPGAVAMVLIWLGLHSLLLRAGVGGVLSALGAREPRDGDQEERQLVNVVEE
                     MAVAAGLRPPRVMLLDSEAANAAAIGKSSEDATVVVSRRVLDELDRDETQGVIGHLVG
                     STGNGDLRISLIIASVFQAYGLLITLVDTPFGAESRAAVMRLLRFATARRSDVGREAE
                     AAAVSALLTRHLGMEGEDDLDRFMEADQAREDSVMKSIRTVLLLPLLFTNLAIKVTLW
                     ITVSFMLGPLIALLWRARRYLADATAVQLTRYPDGLARALDSLGDKGATIPGSSWATH
                     LFVIGSEGSQGGGRGAAAAGSFLTLHPPLKRRLKRLRSLGATSVPEEGRPGGLRVVGN
                     ILAVVVVGPLIVVAAGLLLCLAGIIIGLDLLFMGIAMAVIHGLFSLL"
     gene            2433..3245
                     /gene="murI"
                     /locus_tag="VHM69_16960"
     CDS             2433..3245
                     /gene="murI"
                     /locus_tag="VHM69_16960"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX2742133.1"
                     /translation="MSDPRPIGVFDSGVGGLTVLAEIRDRLPYEHTVYFGDTARVPYG
                     VRDLAEVRWFTFEIINYLIGMDVKMVVIACNTATAAALRATQRSFEVPVIGVIEPGAR
                     AAVEETLNRRVGVLATPLTAASGAYRRAVHSHDAGVEVHEQACPEFVPLIERGVVEGP
                     ELERVARGYLEPLMDRRVDAVVLGCTHYPLISAPINRILGPEVRLISSAGQTALEVGR
                     VLSRRDRLRRPRHIDDEGEATYVCSADPEEFAALGSRFLDEEIKAVAPATVI"
     gene            3284..3357
                     /locus_tag="VHM69_16965"
     tRNA            3284..3357
                     /locus_tag="VHM69_16965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3318..3320,aa:Pro,seq:tgg)
     gene            3384..3454
                     /locus_tag="VHM69_16970"
     tRNA            3384..3454
                     /locus_tag="VHM69_16970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3416..3418,aa:Gly,seq:tcc)
BASE COUNT          582 a         1114 c         1132 g          690 t
ORIGIN      
        1 gctcgcagtg gcaagccgca agaagcagta cgacaagcgg cgtgagatcg ccaagaagac
       61 cgcggaccgc gagatcgaac gcgccatgaa ggagcgcctc cgccgctaga gcgaattgca
      121 gaagtgcctt ctgcaattcg cgcgtgtcag cacggcgcct ttggcgcctt tcagcacgct
      181 tcggccttac ggcctcgctt tcagcttgtc agctttgcct cgaatgacgt tgctcacgct
      241 ccgcggcgaa atctattggg catgagtttc ttctctcgca cggcggtttc tgtatccccg
      301 cccttgctga caagctgaag tgctgaaagc ggaggccgcg ggccgaagcg tgctgatagc
      361 tttgtagaag ccctccaaaa tggtagaata tccactgtac aagggggcgc attggcttcg
      421 acgggagtgg tcagcgtcct gtgagacgag ccgagtttgc tggagactcg taaaacgccg
      481 gcaccacaag atacacgccg ataacgatat ggctctggct gcttaactaa caagtagccc
      541 atcggtccgg gaagcccacg tcccggttct ccggtgtcat tgagtgggat caccctccgg
      601 gttctggccc ggtgcgcggg ggggacattc aacgggctag cctcgcggcg gcgccccggt
      661 ccgtcgcgga aggcgaagtt aaatcgggag ctacgctcgt agaatctcac ggacggcgca
      721 ctcgcggacg cgggttcgaa tcccgccgcc tccactacca ggccgccagg agatcgggcg
      781 gcttttcttc gtccgcagac acacgctact gttaagatgc cggtgtcgtg ccatgttccg
      841 cggaggcgta acgtatggaa cccctggatc ccgcctcgcg cggaggatcg gtgtcgccgg
      901 ttcctggacc ggtagaccgc gagagctttt tcgaggccca gcggcgacac agacgcacga
      961 cgtggcgtct gtctgccgtg tgtgtcgccg cggtcgccgt catgggcatc ccgctcagcc
     1021 tcgttctaac gcccgtgctg tacacgctcg tgctcgtgtt cgcccatctg gcggacctgg
     1081 ttgcgccagt ccctgagagc ttctggcaga cgctcgcggc gggcgcttcc ctgatgatcc
     1141 gggtaatcgg tgggttcctg ggtgaggaag caccggcttc ggtgactcaa acgctcgtcg
     1201 gcctaagcgt catgttactt ccaggcgccg ttgcaatggt cctgatatgg ctcggcctcc
     1261 attcgctgtt gttgcgcgcg ggggtagggg gcgtgctttc ggcgctgggg gctcgtgagc
     1321 ctcgcgacgg cgaccaggag gagaggcagc tcgtcaacgt cgtcgaggag atggcggtcg
     1381 ctgctggcct acgtcctccg cgtgtcatgc tgctcgactc ggaagccgcc aacgctgcgg
     1441 ctatcgggaa gtcttcagaa gacgccaccg tggtcgtttc ccgtagggtc ctcgacgagt
     1501 tggacaggga cgagacccag ggtgtcatcg gacacctggt gggctcaacg gggaatggtg
     1561 atctcaggat ctccctcatt atcgcctcgg tctttcaggc gtatggcttg ctcatcacgc
     1621 tggtggatac cccgtttggc gccgagtcgc gagccgctgt gatgcgactc ttgcggttcg
     1681 cgacggcgcg ccggagcgac gttggccgcg aagcagaggc ggcggcggtc tccgcgctgc
     1741 tgaccaggca tctcggcatg gaaggagagg acgacctcga caggtttatg gaggcggatc
     1801 aggcgcggga ggattccgtg atgaagtcca taagaaccgt cctcctgttg ccgttgctct
     1861 tcaccaacct ggcgatcaag gtgacgctct ggatcacggt ctccttcatg ttgggacccc
     1921 tgatcgccct cctgtggcgc gcccgccgct acctcgccga cgccactgct gtgcagctca
     1981 cacgttatcc tgacggactg gcacgcgccc tcgatagtct cggcgacaaa ggggcgacga
     2041 ttccgggtag tagctgggcc acgcacctgt tcgtgatcgg ctctgagggc tcgcaaggtg
     2101 gaggacgggg cgctgccgca gccggaagct tcctgacgct ccatccgccg ctgaaacgac
     2161 gtctgaagcg cctgcgctcg ctcggcgcaa cgtcggtccc ggaggaagga cgacccggcg
     2221 gcctccgcgt tgtgggtaac atccttgccg tcgtcgtggt cgggcccctg atcgttgtgg
     2281 ccgccggact cctattgtgc cttgcgggca ttatcattgg gctcgacctc ctgttcatgg
     2341 ggattgcgat ggccgtgatc cacggcctgt tctccctctt gtagacgaag gcgccgggta
     2401 aaaggctcct tcagtggata taattcttgc ctgtgagcga tccgcgaccc attggagttt
     2461 tcgactctgg cgtcggtggg ctgacggttc ttgcggagat ccgggaccgt ctgccttacg
     2521 agcacacggt atatttcggg gacacggcac gagttcccta cggggttcgt gacctcgccg
     2581 aggtccgctg gttcaccttc gagatcatca actacctcat cggcatggac gtgaagatgg
     2641 tcgtgatcgc gtgcaacaca gcgacggctg ccgccctgcg ggcgacgcaa cgctccttcg
     2701 aggtacccgt gataggcgtc atagagccgg gcgccagggc cgcggtcgag gagacgctca
     2761 acaggcgcgt cggcgtcctc gcgacgccgc tgactgcggc cagcggggcg tacaggcgtg
     2821 ccgtgcactc ccatgacgcc ggggttgagg tccacgagca ggcatgtccc gagttcgtgc
     2881 cgctcatcga acgcggcgtc gtcgaaggtc ccgagttaga aagggtcgcg cgaggctatc
     2941 tggagcctct catggatcgc agggtcgacg cggtggtgct cgggtgcacc cactatccgc
     3001 tcatctcagc gccgatcaac cgcattctgg gtcctgaggt gcgcctgatc tcctccgcag
     3061 gccagaccgc cctggaagta ggccgcgtcc tctcccgccg cgaccgcctc cgtcgccccc
     3121 gccatatcga cgacgagggc gaggccacct acgtctgcag cgccgacccc gaagagttcg
     3181 ccgccctcgg aagccgcttc ctcgacgagg aaataaaggc cgtcgccccg gcgaccgtaa
     3241 tttaaacacc aacacccctt tgttatactc atgcccggtc tcgcggggcg tggcgcagcc
     3301 tggtagcgca cctgctttgg gagcaggggg tcggaggttc aaatcctctc gccccgacag
     3361 cgaagccggc gcaaagccac cgtgcgggtg tagctcaacg gtagagcccc agccttccaa
     3421 gctggtgatg ggggttcgat tcccctcacc cgctctcgcc cggcacttca gcaagtcagc
     3481 ctgtcagcac gccgcggcct ccccttctca gcgcatca
//
LOCUS       DATAIR010000713         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_350845, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000713 DATAIR010000000
VERSION     DATAIR010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350845"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VHM69_16975"
     CDS             <1..714
                     /locus_tag="VHM69_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX2742134.1"
                     /translation="GAARGLTSELSRAGVEVTGAVPGSGIGELPPLGEGTVMAVMDPN
                     LTTAAAEAERRGATVVRTLMPVGVDGTARFVQDVAEEAGSRISEVVRARSAWEDLGAL
                     RNRIRGKRIFFAGDTGMEIPLARFLADAGAVVLEVGAPRLDRRFLAAELQALGPDVDV
                     VVSPDYRGQTKRIEEARPDVVISSPALHLPLVARGHLCRSPQDFLVAGLYGYGGARRI
                     LELLVRTLERAETLDALNL"
     gene            711..2135
                     /locus_tag="VHM69_16980"
     CDS             711..2135
                     /locus_tag="VHM69_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX2742135.1"
                     /translation="MKLLRGVYEGPGSHGILRVGASIRGVHAVLRALPGETYFPTLYG
                     TRERTGEMAPVTLSPLHEGPENSMAPGNVARDLSSVVRRHPRTETVILVRSEASILSG
                     ETVPVTSLPEIEGRTPQLVTFSWEGPGMRETEAAELALEDLIRAHLQVAPERSPYPTV
                     NLFGPPVFGPGAAAEYAEAERLLDLIGVEVNARVPLGTGAGDLGRLPRAWANVLLYRE
                     VGESATLFLQDEFGMPRVTTPMIGAAGTGAALRAVGELCQLDPKSVQRAIWAELARTA
                     KLPWYARLEPPETFRGRRAAIFGDFTYPLGLGYAMAREVGLEVTACGTYLTHLERDFL
                     FHAHTFTEDAFVEEDPEEVAARIEAAKPDLVVGTHLEEGVADSLGVPFLPLCHPTAGR
                     HFVERPLMGYAGSSVIADALEGALPGTRTESTNEAGMQWTDGALQEVAEVPPFLRGRA
                     RLLAEERARQQGSGEVTREIFLDS"
     gene            complement(2435..2716)
                     /locus_tag="VHM69_16985"
     CDS             complement(2435..2716)
                     /locus_tag="VHM69_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742136.1"
                     /translation="MTKEQSVFGQFFFGDPSRAMREERVLQYVIHRINEGAVLHEVLE
                     EPYVQRNCSQIEVDEICSNPELVHACRERLEETFRSGELDPMRHRAPGT"
     gene            complement(2719..3078)
                     /locus_tag="VHM69_16990"
     CDS             complement(2719..3078)
                     /locus_tag="VHM69_16990"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX2742137.1"
                     /translation="MRRRQSRSEASISDEMMAFQHEFVGRGPDRIRTRIVEDLVIVRS
                     FGVFTPAEKQLATSFEGRRIIKAMRQQVLEAGRSVLERIVRKHTEAEVITVHSDISTK
                     SGEWLDIFVLDRSVEEE"
     regulatory      complement(3303..>3364)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          576 a         1103 c         1086 g          599 t
ORIGIN      
        1 ggtgctgcgc gaggactcac atcggagctc tcaagggctg gagtggaggt gacaggggcc
       61 gtgccggggt cgggcatcgg ggagctcccg ccactgggag agggcacggt gatggcggtg
      121 atggacccga acctgacgac ggcggccgct gaggccgaga ggcggggagc cactgttgtc
      181 aggaccctca tgcccgtcgg cgtggacggt actgcccgct tcgtgcagga cgtagccgag
      241 gaggcggggt cgcggatcag cgaggtcgta cgggcacggt cagcatggga ggaccttggg
      301 gccttgagaa accgcatcag gggaaagagg atcttcttcg ccggcgacac ggggatggag
      361 atcccgctcg cccgtttcct cgccgacgcg ggggctgtgg tactggaggt cggcgcgccg
      421 cggctcgacc gcaggttcct ggccgccgag cttcaggccc tcggccccga cgtggacgtg
      481 gtcgtctccc cggactacag ggggcagacg aagcgcatag aagaagccag gcccgacgtg
      541 gtgatctcga gcccagctct ccacctgccg ctcgtcgccc gcggacatct atgccgctca
      601 ccgcaggact tcctggtggc cggtctctac ggttacgggg gcgcgcgtcg catactcgag
      661 ctcctggtgc gcaccctgga gcgcgcggag accctcgacg ccctgaacct gtgaaactcc
      721 tgcgcggcgt ctatgagggg ccgggaagcc acggaatcct gcgggtgggc gcgagcatca
      781 ggggcgtgca cgccgtactc agggcgctgc cgggcgagac gtacttcccg acgctttacg
      841 ggacacgtga gcggacaggg gagatggcgc ccgtcacgct aagcccgctg cacgaaggcc
      901 ccgagaactc catggcgcca gggaacgtcg cccgcgacct ctcctctgtc gtccggcgtc
      961 acccgaggac ggagaccgtt atcctggtcc gctcggaggc ctcaattctc tccggggaga
     1021 ccgtccccgt aacctctctc cccgagatag aagggagaac cccgcagctg gtgacgttct
     1081 cctgggaagg gcccggcatg agggagacgg aggccgcgga gctcgcgctg gaggatttga
     1141 tccgggccca cctgcaagtc gcacccgagc ggagtccata ccctacggtc aacctcttcg
     1201 ggccgccggt cttcggtccc ggcgcggcgg ctgaatatgc ggaggccgag cggctcctcg
     1261 acctgatcgg ggtcgaagta aacgcgaggg taccgctcgg gacgggcgcg ggagacctcg
     1321 gccggctccc gcgcgcgtgg gcgaacgtgc tcctttacag ggaggtcggg gagtctgcta
     1381 cgctcttctt acaggacgag ttcgggatgc ctcgggtgac gaccccgatg atcggcgccg
     1441 ccggaaccgg cgcggcgcta cgggcagtcg gcgaactgtg ccaactcgac ccgaaatccg
     1501 tgcaacgggc gatctgggcc gagctggccc gcacggcgaa acttccgtgg tacgcccggc
     1561 tcgagccgcc cgagactttc aggggccgca gggccgccat cttcggggac ttcacctacc
     1621 cactaggcct cggttacgcc atggcccgcg aggtcggcct ggaggtaacg gcctgcggga
     1681 catatctcac gcacctcgaa cgagatttcc tttttcacgc gcacaccttc acagaagacg
     1741 ctttcgtcga ggaagatccg gaggaggtcg ctgcccgtat cgaagccgcg aagcctgatc
     1801 tggtcgtagg gactcacctg gaggagggcg tggccgattc cctgggtgta cccttcctcc
     1861 ccctctgcca tcccaccgca ggccgtcact tcgtcgagcg accgctcatg ggctacgcgg
     1921 gctcaagcgt catcgccgac gccctcgaag gcgccctgcc gggaacgaga acggagtcca
     1981 caaatgaggc cggcatgcag tggaccgacg gggcgctaca ggaggtggcc gaggtaccgc
     2041 cgttcttgcg gggcagggcc cgtctgctcg ccgaggagcg tgcccgccag cagggctccg
     2101 gagaggtgac gcgggagatc ttcctggatt cttgatgcat cgacgtggtt caatattgtc
     2161 ctggacctgg cgcgatcgtc cggccaggga tcttcgatca gacctgctcg atgcgctcgg
     2221 caagcaacca gcgattgaag cgctatgcga catgagtcgg cgaaaccgtt ggctgggcca
     2281 ctacgaccct tcttccactg gcacgccagt cgtccgcatc aacgatcggg cgatagatgg
     2341 attcgcgtta cgttcatcac aggaaactcc ctcccgaatt gccccccgtg cgatcgcttc
     2401 ttgctctgac aggcctgctc ctggctgacg acctctaggt gccaggggct ctgtgccgca
     2461 tggggtccag ctctcccgag cggaatgtct cctccaggcg ctccctacag gcatggacga
     2521 gctcagggtt gctgcagatc tcatctactt caatctgcga gcagttgcgc tgtacgtaag
     2581 gctcctccag cacctcgtgg aggacagcgc cctcgtttat acggtggatg acgtattgga
     2641 ggaccctctc ctccctcatg gccctactcg ggtcgccaaa gaagaactga ccgaagacag
     2701 actgttcttt cgtcaccgct attcctcctc tacactgcga tctagcacga agatgtccag
     2761 ccactcccca ctcttggtgg agatgtccga gtgaacggtg atgacctcag cttccgtatg
     2821 cttccggact attctctcca ggaccgaacg cccggcttcg aggacctgct ggcgcatagc
     2881 cttaatgatc ctgcgcccct cgaagctggt ggccagttgc ttctcggcag gcgtaaatac
     2941 gccgaagctg cgcactatca ccaggtcttc tacgatgcgc gtcctgatcc tgtcaggacc
     3001 acgtcccacg aactcgtgct gaaaggccat catctcgtcg ctgattgacg cctcgctcct
     3061 gctctgcctg cgtctcatgg acgtgtcgct gcacctcctc tgagggtgcg gcaacgacga
     3121 aggccggtgc cgcaccgcac ctctacacca atagagggga cgcagtcacc ggccttctat
     3181 ccgctggccg ccccggagta cgcaccgaga aggtcgccga gatatagccc gactgtggct
     3241 gaccacccct gcctcccgat ccggcgccgg ttctcccaac agcaaaccac ttggctcgga
     3301 gtgccctctt tccttatcgc ctacgaggtt agctgtcggg ttcgggccga aagagttaag
     3361 ccct
//
LOCUS       DATAIR010000714         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_350936, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000714 DATAIR010000000
VERSION     DATAIR010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350936"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VHM69_16995"
     CDS             complement(<1..842)
                     /locus_tag="VHM69_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2742138.1"
                     /translation="MYPDFIGIGAQKAGTTWLSHNLQHHPQIWMPPLKEIHYFDERLD
                     EPANLVPRLSRRLSGGRGVDRRWRRQVKSRIGRHLKQFSKKRFLWDVRYYFGAPGDDW
                     YASLFEPGKGRVVGEITPAYSMLEPATVAHVHEVVPQAKIVFMMRNPIERAWSQIAMR
                     FNKAGERDIASVKDKRLQRNFEREGSRSRTNYLRTLETWGSFYPEEQIFVGFLEDVHF
                     FPEALLSRAYEFLGVDSSFKPPGVEQRVHSRSTGQIPTRMVVYLAHTYLEQLQDLHER
                     FGGYA"
     gene            complement(873..1865)
                     /locus_tag="VHM69_17000"
     CDS             complement(873..1865)
                     /locus_tag="VHM69_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2742139.1"
                     /translation="MYPDFLGIGAQKAGTTWLHRNLQEHPQIWMPREKELHYFDEKLK
                     LEGGLLTRLRSDRPVDRRWRRQCASRFRHLPNKEFWKDLTWDLRYFFFPPNDKWYASL
                     FEPGRGKITGETTPDYSILDTDTIKHVHHIMPQAKIIFFMRNPIERPWSAADMGLRIS
                     GRSLEEVPDRKVYRQFDRRRVRLMTNYMRTIENWGSFYPEEQIFVGFLEDIHLAPVRL
                     LRNLYRFLGVDPSVKYRVMTRKIHSGQQDTMPTRFAVYLAHTYLEQLQDLHERFGGYA
                     SFWLYCAERLVNDPPEEEHIPYPLWESVLWEEWEGSRDMIKVRSGPLSSLGATS"
     gene            complement(1897..2889)
                     /locus_tag="VHM69_17005"
     CDS             complement(1897..2889)
                     /locus_tag="VHM69_17005"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2742140.1"
                     /translation="MYPDFLGIGAQKAGTTWLARNLQSHPQIWMPRKEVHYFDRKIND
                     RSNAISRLFGKRDVDAQWRRHVRTWAFRHAIKQPSLRNLLWDFKYYMRPYNDEWYLSV
                     FDGKGERIAGEITPAYSVLDKDQVAHVHSVAPEAKIIYFMRNPIERAWSNAVMSFDKA
                     EKGSADSVTEGELLKRLERSSSSRLTKYLRTIENWGAYYPEERVFVGFLEDIHFFPEE
                     LLGRLYSHLGVDPYFHRSLTNKKIHSRSGGTMPTSIAVHLAQNFQDEIAQLSVQFGGY
                     ASFWLYCAERLIESPPQEESIPYPLYESRMWEEWGGSRQMEIQSGPLTSARVIG"
     gene            complement(2990..4006)
                     /locus_tag="VHM69_17010"
     CDS             complement(2990..4006)
                     /locus_tag="VHM69_17010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2742141.1"
                     /translation="MGEWRRMYFMTPTFRPVGGVIKIFDYVNHALSLGYEPVIACPES
                     FKPGLPLFGISRFSHITPENGFLFTDLEKVTVGPHDLAFLSWPTHYEIVEPRLSRWTR
                     HEQVVFIVQNVRHANPTFTGGYALRLLSRPMARIMTNDVVLEAVRPYLNGTSMTEVIQ
                     LGNESSYFAKERTGGFVNPVRVGYTTWKSGVGDEVAQMLSDDPGFKFRAIREPVGWKE
                     LKDLYQWSDVFLATPLVEEGFYMPGLEAMAAGAVVISSDAGGNRAYCRFGENCVEVGF
                     EDARDYVGALEGLREGGVESIERLRGAGYEAVGRHTLEHERERFGQFMEKLTERLDLL
                     GPKL"
     gene            complement(4007..5161)
                     /locus_tag="VHM69_17015"
     CDS             complement(4007..5161)
                     /locus_tag="VHM69_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742142.1"
                     /translation="MVSKRVFVISNDIVPSLGMPVAAPGLRVFGLAEGLRAHGFEVKT
                     VVSKGFVERQWLRFGKPVPHPTAPDTEIIGASRLARYLEAHAPAVVILINSNQVDHLK
                     PIKGIHYVLDFFAPKMLEMLYQHGEGYPEDELKRLRQRKIRAIRMADAFVSNGAKKLP
                     YFLAWMLQADRDVRGLPLEVVNMCVPLSLTEDGTRNDGVRFAVAGYLQSWSTLGNWVD
                     VLESRLDRPGVKLDLLVPWHWSRREHLHESKSDLDRIGGHESVTTHGPMTFSNFQKFL
                     STVDVTIDLFQHNLEREYAMVTRSVVSLAAGVPVIHPPFTEVSEMISEYDAGWLVDPR
                     DTGALENVLDEIIESPDVLLRKTKNARTLARAVIDPAEAVRPLVRIMEGW"
     gene            complement(5155..>5815)
                     /locus_tag="VHM69_17020"
     CDS             complement(5155..>5815)
                     /locus_tag="VHM69_17020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2742143.1"
                     /translation="WRDCGDALALSAPYRTVFGAHHDTAFGKAVHRVQADALEPQFAA
                     ARGDRAADIPFEVLEVGCGIGRWAAGFDPTTTRFAGVDISGGMVEAARANFPEGRFDL
                     LEDDLLFPYAEESFDLVFSVGVMHHNPTQAKRALLSEMWRVTRPGGRLLFLEDFVAES
                     GSSRSIIYPMSVLKFVDVILEETAGQVVLEYVEALRYPRDPFFRGGVIALTKLGVPKT
                     W"
BASE COUNT         1118 a         1803 c         1701 g         1193 t
ORIGIN      
        1 gcgtaccctc cgaatcgctc gtgcagatcc tgaagttgct cgaggtaggt gtgtgccagg
       61 tagacaacca tacgcgtcgg tatctggccc gtggaccgtg aatggaccct ctgttccacc
      121 cccggtggct tgaaggagga atccacgccc aagaactcgt acgctcgact caagagggcc
      181 tcggggaaga agtgtacgtc ttcgaggaac ccgacgaaga tctgctcctc aggataaaaa
      241 gagccccagg tctctagcgt cctcagatag tttgtcctcg aacgcgagcc ctcacgctcg
      301 aagttccgtt ggagtctctt gtcctttaca gatgcaatgt cacgttcacc ggccttattg
      361 aatcgcatcg cgatttggga ccatgcgcgt tctatcgggt tgcgcatcat aaagacgatc
      421 ttcgcctgcg gcacgacctc gtggacgtgg gcgacggtag caggctcaag catggagtag
      481 gctggagtga tctcaccaac cacccttccc ttgccgggct caaatagcga ggcgtaccaa
      541 tcgtcgccgg gtgcgccaaa atagtaccgt acgtcccaaa gaaatcgttt cttggaaaac
      601 tgcttcagat gccgcccaat acggctcttg acctgccggc gccaccgacg atcaacgcct
      661 cgcccgccgg agagtcttct ggaaagcctg ggcacgaggt ttgcgggctc gtcgagcctt
      721 tcgtcgaaat aatggatctc ctttagaggg ggcatccaga tctgggggtg gtgctgcaga
      781 ttgtggctca accacgtcgt gccggccttc tgggccccta tcccgataaa gtccggatac
      841 aaatcgccgc tcccgttacg aaggcgtggc cttcaagaag tggctccgag agacgataac
      901 ggcccgcttc gcaccttgat catatccctc gatccttccc actcctccca gaggactgac
      961 tcccacaggg gatacggtat atgctcctcc tcgggagggt cgtttacgag cctctcggcg
     1021 cagtacagcc agaaagacgc gtaccctccg aaccgctcgt gcagatcctg aagttgctcg
     1081 aggtaggtgt gtgccaggta gacggcgaat ctcgtaggca ttgtatcctg ttgacctgag
     1141 tgtatcttgc gcgtcatgac cctgtacttc acagaaggat ccacgccaag aaatcgataa
     1201 aggttccgca gcagcctcac cggagccaag tggatgtctt cgaggaaccc gacgaagatc
     1261 tgctcctccg ggtagaacga tccccagttc tcgatcgtcc gcatgtaatt ggtcatcagc
     1321 ctgacccgcc tgcggtcgaa ctgcctgtaa acctttctgt caggcacctc ctccagagat
     1381 cgtccggaga tcctgagccc catgtctgcg gcagaccagg gtctctctat cgggttgcgc
     1441 atgaagaaga ttatctttgc ctgcggcatg atgtgatgga cgtgcttgat cgtgtcagtg
     1501 tccaggatcg aatagtccgg cgtcgtctcc cccgtgattt tgcctctgcc aggctcgaag
     1561 agggaagcgt accatttgtc atttggcggg aagaagaaat acctaagatc ccacgtcaga
     1621 tccttccaaa actccttatt cggcaagtgt ctaaacctgg aagcgcattg tcggcgccaa
     1681 cgcctgtcga caggccggtc actgcggagt ctggtcaaga gtccgccttc tagcttcaac
     1741 ttctcatcaa aatagtgcag ttccttttcc ctgggcatcc agatctgcgg gtgctcctga
     1801 aggttacgat gcagccacgt cgtgcccgcc ttctgcgctc ctatgcctag gaaatccggg
     1861 tacatctcag tttcctatcg gggcggccag gcgtactcaa ccgatgaccc gcgcggaggt
     1921 caggggtccg ctctggatct ccatctgcct ggatcctccc cactcctccc acatcctcga
     1981 ctcgtagagg ggatatggga tgctctcttc ttgcgggggg ctctcgatga gccgctcggc
     2041 gcagtagagc cagaacgacg cgtacccgcc gaactggacg ctcagctgcg ctatctcgtc
     2101 ctggaagttc tgcgccaggt gcacggcgat gctagtcggc atcgttcccc cggacctgga
     2161 gtgaatcttc ttgttcgtga gtgaacgatg aaaatagggg tccacgccca ggtgtgagta
     2221 gaggcgaccg agaagctcct cggggaagaa gtgtatgtcc tcgaggaatc cgacgaaaac
     2281 ccgttcctca gggtagtacg cgccccagtt ctcgatggtc cgcaggtact tagtcaaccg
     2341 cgagctactg ctacgctcca gcctcttgag cagctctcct tcagtgaccg agtcggccga
     2401 acctttttcg gccttgtcga agctcattac ggcgttcgac caggcgcgct ctatagggtt
     2461 acgcataaag tagatgatct tcgcctcagg cgccacgctg tggacatggg cgacctgatc
     2521 cttgtcgagt accgagtagg ccggtgtgat ctcacccgct atcctctcac ccttgccgtc
     2581 gaagacggat aggtaccact cgtcattgta gggcctcatg tagtatttga agtcccacag
     2641 aaggttccga agggaaggtt gctttatcgc gtgcctgaag gcccaagtcc ttacatgccg
     2701 gcgccactgc gcgtcgacgt cgcgcttgcc gaagagcctg cttatggcgt tggagcggtc
     2761 gttgatcttc ctgtcgaagt aatggacctc ttttctgggc atccagatct gcggatgcga
     2821 ctgaaggttg cgggccagcc acgtcgtgcc cgccttctgt gcccctatac ctaggaaatc
     2881 cgggtacatc tcgatcctcc tgtaattcgt ccgctagtag tgtcgggctc aacgcgaccg
     2941 acaaataatc gagttgggag gcggttttgt caagccagtg taccgaagat caaagcttcg
     3001 gaccgagaag atcgagacgc tccgtcagct tctccatgaa ctggccgaaa cgctcccgct
     3061 cgtgctcgag ggtgtgcctt ccgaccgcct cgtagccggc tcctcgcagt cgctctatgg
     3121 actcaactcc accctcgcgc agaccttcga gggccccaac gtagtccctg gcgtcctcga
     3181 agcccacctc tacgcagttc tctccgaagc ggcagtaggc gcggtttccg ccggcgtccg
     3241 aagagatcac cacagcaccg gcggccatcg cctcgagccc tggcatatag aacccctcct
     3301 caacgagcgg cgtcgccaga aacacgtctg accactggta gaggtccttc agttccttcc
     3361 atccgacagg ctcccttatc gccctgaact tgaagccggg gtcgtcggag agcatctggg
     3421 ccacctcgtc gcccaccccg ctcttccagg tcgtgtagcc gacgcggact ggatttacga
     3481 acccgcccgt cctctccttc gcgaaatagc tcgactcgtt gccgagctgg atcacctcgg
     3541 tcatcgaggt cccgttcagg tagggcctga ccgcctcgag gaccacgtcg ttagtcatga
     3601 tgcgggccat ggggcgcgag agcagacgca gcgcgtaacc acccgtaaaa gtcgggttgg
     3661 cgtggcggac gttctggacg atgaagacca cctgttcgtg gcgcgtccac cggctgaggc
     3721 ggggctcgac tatctcgtag tgcgtgggcc atgataggaa ggcgaggtcg tgcggcccga
     3781 cggtcacctt ctccaggtcg gtgaacagga acccgttctc cggagtgatg tgtgaaaagc
     3841 gcgagatacc gaagaggggc agaccgggct tgaatgattc tgggcaagcg atcacaggct
     3901 cgtaacccag cgagagcgcg tggttgacgt agtcgaagat cttgatgacc ccgccaactg
     3961 gacggaacgt cggggtcatg aagtacatgc gccgccactc gcccatttac caaccctcca
     4021 tgatgcgaac cagcggcctc acggcctcgg ccgggtctat gaccgcgcgc gccagcgtcc
     4081 tggcgttctt ggtcttccga agcaacacgt ctggagactc tatgatctcg tcgagcacgt
     4141 tctcgagcgc ccctgtatct cgtgggtcaa ccagccatcc ggcgtcgtac tcggagatca
     4201 tctcggagac ctctgtgaag gggggatgta tgacggggac cccggccgcc agggagacga
     4261 ccgagcgggt gaccatggcg tactccctct ccaggttgtg ctggaagagg tcgatcgtca
     4321 cgtcgaccgt cgagaggaac ttctggaagt tcgagaacgt catggggccg tgcgtcgtca
     4381 cggactcatg gccaccgatc ctatcgaggt cgctcttgga ttcgtggagg tgctccctcc
     4441 tgctccagtg ccacggcacg aggaggtcga gcttcacccc tggccggtcg agcctgcttt
     4501 cgagtacatc cacccagtta cccagcgtgc tccaggactg cagatagcct gcgacggcga
     4561 acctgacgcc gtcgttccgg gtgccgtcct cggtcagcga cagcggaacg cacatgttga
     4621 cgacctccag gggcaggccg cggacgtccc tgtcggcctg cagcatccag gcgagaaagt
     4681 aaggcagctt cttcgccccg ttgctcacga aggcgtcggc catgcggatg gcgcggatct
     4741 tgcgctggcg tagccttttg agctcatcct cagggtatcc ttctccgtgc tggtagagca
     4801 tctccagcat cttcggcgcg aagaaatcga gcacgtagtg gatgcctttt atgggtttca
     4861 ggtggtcaac ctggttgctg ttgatcagta tcactaccgc gggcgcgtgg gcctcgaggt
     4921 agcgggcgag cctgctagcg ccgattatct cggtgtcggg ggccgtcgga tggggcacgg
     4981 gctttccgaa cctcaaccac tgccgctcca cgaagccctt ggagaccacg gtcttaacct
     5041 cgaagccgtg ggcgcgcaga ccctcggcga gcccgaagac ccgcaagccg ggtgctgcga
     5101 ctggcatccc cagactaggg acgatgtcgt tggagatcac gaagaccctc ttgctcacca
     5161 cgtcttcggc acccccaact tggtcagcgc gataaccccg cctcggaaga aggggtccct
     5221 ggggtagcgg agcgcctcga cgtactccag caccacctgc cccgcggtct cttcgaggat
     5281 cacatccacg aacttcagca ccgacatagg atagatgatg gacctggacg aaccactctc
     5341 ggcgacgaag tcctccagga acagcagccg cccccctggc cttgtcacgc gccacatctc
     5401 ggagagcagt gcgcgcttgg cctgcgtggg gttgtggtgc atgacgccca cgctgaagac
     5461 caggtcgaag ctctcttcgg cataggggaa gagaaggtca tcttcgagga ggtcgaaacg
     5521 tccttcgggg aagttggcgc gggcggcctc caccatgcct ccgctgatat ccaccccggc
     5581 gaacctggtg gtcgtgggat cgaagcccgc agcccagcgc ccgatcccgc agccgacctc
     5641 gagtacctcg aacgggatgt cggccgcgcg gtcccccctg gcggcggcga actggggttc
     5701 cagcgcatcc gcctgcacgc gatgcacggc cttgccgaag gccgtgtcgt gatgtgcgcc
     5761 gaagaccgta cgatatggcg cggagagagc cagcgcgtca ccgcaatccc tccag
//
LOCUS       DATAIR010000715         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_213239, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000715 DATAIR010000000
VERSION     DATAIR010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213239"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VHM69_17025"
     CDS             complement(<1..1111)
                     /locus_tag="VHM69_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2742144.1"
                     /translation="MEDALTVSRLQFAFTVTYHYLFPMLTMGLALLIFILKSVYMRNR
                     DELYNRSARFWAKIFAVTFVMGVVTGIPLEFQFGTNWAAFSRFAGDIIAQTLAMEGAF
                     AFFLESAFVGLFLFGEQRFGQRVHWFSSLMVFLGTWASGYFIITTNAWVQHPVGYRVL
                     ENGNIELDNYWAVLLNSWVWPQYAHNMGGAVVCGAFVMAGLGAYYLLARRHEEYGRIF
                     VKVGVITAAIASLWMLFPTGHFSSEQVAEHQPVSLAAMEGLFESERQAGIVLVGQPDV
                     ENRKIENPIVVPRALSYLIYQNWNAEVKGLDAFPEKNWPDNIPLLYYSYHVMVGLGTI
                     FIAIMVAAAFLLWRGRLYGSRPMLWVLMLAIPLPFI"
     gene            complement(1137..1616)
                     /locus_tag="VHM69_17030"
     CDS             complement(1137..1616)
                     /locus_tag="VHM69_17030"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX2742145.1"
                     /translation="MGDFFHLTHVMLAGVWLGGLVFTTFVVSPALKAMKWPEAERVAV
                     RTAIGRQYARVGSVNLGLLLLFAILDGVSSGFGADLYAEYALLLVLFGLVAAHGAYFG
                     RHLAKLAEAEREAESEEVARHFADRRRSLQKVSSRVSWMTLLVSTVVMVLATGAQAG"
     gene            complement(1752..2336)
                     /locus_tag="VHM69_17035"
     CDS             complement(1752..2336)
                     /locus_tag="VHM69_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742146.1"
                     /translation="MGVHIEVFLAVAYAAFLLCVAIGLDLLARHSHARSQRYRTAGFT
                     YHHKHDAWICPEDQILTRSETDHERRLIRYRGRPHICNNCPSKGECTDSNEGREVVRP
                     MDPWPHSEAGRFHRGISVCVALIAALILAAEVLRNHAPLELAVLGAALLVVSLVVWHL
                     HAAFRSSPANFPVPRVVHEENLRTGYRRGKEARR"
     gene            complement(2341..>4099)
                     /locus_tag="VHM69_17040"
     CDS             complement(2341..>4099)
                     /locus_tag="VHM69_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2742147.1"
                     /translation="FAWTSWAASTLRPGLDYTYTNNWPPEPLVENEPTANVVVWSVLS
                     LIALLGGIGLLFAAFGRWNFLGWRGREQATLSFRAPGDVALTPAQRACAWFFLVMAAL
                     FLIQTLVGAASQHYRADLASFFGMDLARWLPYNLMRTWHVQLAIFWVATSFLAAGIFL
                     APMIAGREPKGQRWLAFGLLGALALVVFGSMIGEWLGIFGLMGEWWTLFGNQGFEYLD
                     LGRFWQILLSIGLFFWVAMLFRAMRGRLRREHMGNMPWLFFFAALAIPAFYAVGLLAR
                     PESHFTTADFWRFWVVHLWVEDFLELFTTVMVAYIFVLLGVVREKVALTVIYLDILLY
                     SAGGVIGTMHHLYFSGTPAEHMALGAFFSAAEVIPLTFLTVEAWSFLQLGARQESRSP
                     TPFPHRWAVMFLVAVGFWNFLGAGIFGFLINLPIISYYQIGTALTANHGHAAMMGVYG
                     MLAAGLALFCLRYLIPEERWSERAAKISFWSLNIGLAWMSFATLFPLGILQLYESVSV
                     GYYEARTLEFLTNDTNALLEWMRLPGDVVFIVGGVLPLLYLCYLGVRHTVPRVTMEEP
                     EDILFTDITEPEVAGPRER"
BASE COUNT          871 a         1265 c         1289 g          674 t
ORIGIN      
        1 cgatgaaagg taagggaatg gcgagcatca ggacccagag catgggacgc gacccataga
       61 gcctcccacg ccagagtagg aacgccgctg cgaccatgat ggctatgaag atggtcccga
      121 ggccgaccat gacgtggtag ctgtagtaca ggagggggat gttgtcgggc cagttctttt
      181 cggggaaggc gtcgaggccc ttcacttcgg cgttccagtt ctggtagatg aggtagctga
      241 gggcgcgcgg cacgacgatg gggttctcta tcttcctgtt ctccacgtcg ggctgtccaa
      301 cgagaacaat gccagcctgc cgctctgact cgaagagccc ctccatggcg gccagggaga
      361 ccggctggtg ctctgcgacc tgttcgctcg agaagtggcc cgtcgggaag agcatccaga
      421 ggctcgcgat ggcggcggtt ataacaccga ctttgacgaa gattcgtccg tactcctcgt
      481 gcctgcgcgc gagcaggtaa taggcgccga ggccggccat aacgaaggct ccgcacacca
      541 cggccccgcc catgttgtgg gcgtactgcg gccacaccca cgagttgagg aggacggccc
      601 agtaattgtc gagctcgatg ttgccgttct cgagcacgcg gtagccgacg gggtgctgca
      661 cccaggcgtt cgtagtgatg atgaagtagc ccgaggccca ggtaccgagg aagaccatca
      721 gggacgagaa ccagtggacc ctctgcccga agcgctgctc tccgaagagg aacagcccca
      781 cgaacgctga ctccaggaag aacgcgaagg cgccctccat cgccagagtc tgggcgatta
      841 tgtcccccgc gaagcgggag aaggcggccc agttggtccc gaactggaac tccaggggta
      901 tgcctgtgac tacccccata acgaaagtga cggcgaagat cttcgcccag aagcgcgcag
      961 acctgttgta tagctcgtcc cggttgcgca tgtagacgct cttgaggata aagatcagga
     1021 gcgccagccc catggtgagc atcgggaaga ggtagtggta ggtcacggtg aacgcgaact
     1081 gcagacggga tactgttagc gcgtcttcca aagccacctc ctacgatacc gccgcattat
     1141 cccgcctgtg cgccagtggc gaggaccatg acgacggtgc tgactagcaa ggtcatccag
     1201 gagactcgcg aggagacctt ctgcaacgag cgtcgcctat cagcgaagtg cctggccact
     1261 tcttcactct ccgcttcccg ctctgcctct gccagcttcg ccagatgccg cccgaagtat
     1321 gcgccgtgcg cggccacgag accgaagagc acgagtagca acgcgtactc cgcgtagagg
     1381 tctgcaccga aaccactgga gactccgtcc agaatcgcga agagtagtag cagccccaaa
     1441 ttgacactcc ctaccctggc gtattgtctc cctatcgccg tgcgcacagc gaccctttcg
     1501 gcctcgggcc acttcatcgc cttcagggcg ggcgagacta cgaaagtcgt gaacaccagc
     1561 cctccgagcc agacgcccgc cagcatcacg tgcgtcaagt gaaagaagtc gcccaacgct
     1621 cctacctcct gactattgcg gtttcttcta tgtccgatca acggctgccc ggcgcgtcca
     1681 ggctccgtgc accgcctgac tgtaacggta gcggaggcga cccgcctgag tagatttgcc
     1741 taatttgacc atcaccgtcg ggcctcttta ccccttctgt agcccgtgcg caggttctcc
     1801 tcatgcacga cccggggcac cgggaagttg gcgggggaac tgcggaaagc cgcgtgaagg
     1861 tgccagacta ccaacgagac gaccaagaga gcggcaccca ggacggccag ttccaggggt
     1921 gcgtggttgc ggagtacctc ggcggcgagg atcagggctg ctatgagggc gacgcagacc
     1981 gagataccac ggtggaaacg ccccgcctcg gagtgaggcc aggggtccat ggggcgcacg
     2041 acctctcgtc cttcgttgga gtcggtgcat tcgcctttgg agggacagtt gttacagata
     2101 tggggcctcc cccgataacg tatcaggcgc cgctcgtgat cggtctcgct gcgggtgagg
     2161 atctggtcct cagggcagat ccaggcgtcg tgcttgtgat ggtaggtgaa cccggcggta
     2221 cgatagcgtt gcgagcgggc gtgcgagtgt cgggccagca ggtcgaggcc gatcgccacg
     2281 cacaggagga atgcggcgta ggctaccgcg aggaacactt cgatgtgtac gcccatcgtc
     2341 ctaacgctcc ctcggtcctg ccacctctgg ctcggtgata tctgtgaaca ggatgtcctc
     2401 gggctcctcc atcgtcaccc gtgggacagt gtggcgcacg ccgaggtaac agaggtaaag
     2461 gaggggcaac acgcccccga cgataaagac tacgtcccca ggaaggcgca tccactccag
     2521 gagggcgttg gtgtcgttgg tcaggaactc cagcgtgcgg gcctcgtagt agccgacgct
     2581 gacggactcg tagagctgca agataccaag cggaaatagg gtggcgaagc tcatccaggc
     2641 cagcccgatg ttcagcgacc agaagctgat cttcgccgcc cgctcggacc agcgctcctc
     2701 ggggatcaaa taccgcaggc agaacaaagc tagccccgcc gcgagcatcc cgtagacgcc
     2761 catcatcgcc gcgtggccgt ggtttgcggt aagtgctgtc cctatctggt agtaggagat
     2821 gatcggcaag tttatgagga acccgaagat acccgctccg aggaagttcc agaagccgac
     2881 cgccaccaaa aacattacgg cccagcgatg cggaaacggc gtcggggagc gcgattcctg
     2941 cctggccccg agctgcagga agctccaggc ttcgacggtc aggaacgtca gggggatgac
     3001 ttccgcagcc gagaagaaag caccgagcgc catgtgctcg gccggcgtgc ctgagaaata
     3061 gaggtggtgc atcgtcccga tcacaccccc cgccgagtac agcagtatgt ccaggtatat
     3121 gacggtcaat gccacctttt cgcgcacgac gccgagcagg acgaagatgt aggcgaccat
     3181 gacggtcgtg aagagctcga gaaagtcttc cacccagagg tgtacgaccc agaagcgcca
     3241 gaagtccgcc gttgtgaagt gcgactccgg cctggcgagc aatcctacgg cgtagaaagc
     3301 ggggatcgcc agggcggcga agaagaacag ccagggcatg ttgcccatgt gctcgcgccg
     3361 caagcgtccc ctcatggccc tgaacagcat cgcgacccag aagaagaggc cgatggagag
     3421 gagtatctgc cagaagcgcc cgaggtcgag gtactcgaag ccctggttgc cgaagagtgt
     3481 ccaccactca cccatcagtc cgaagatgcc tagccactcc ccgatcatgc taccgaacac
     3541 cacaagcgcc agcgctccca acagcccgaa tgcgagccag cgttgtcctt ttggttcgcg
     3601 acctgcgatc atcggcgcca ggaagatccc ggccgccaga aacgaggtcg cgacccagaa
     3661 gatcgccaac tgcacgtgcc aggtgcgcat gaggttgtaa ggtagccacc gcgcgaggtc
     3721 catgccgaag aagctcgcga ggtcagcccg gtaatgctgc gaggcagccc caacgagtgt
     3781 ctgtatgaga aacagcgccg ccatgaccag gaagaaccac gcgcacgccc tctgggccgg
     3841 cgttagagcc acgtcgccgg gggcgcggaa cgacagcgtc gcctgctcgc gcccgcgcca
     3901 accaaggaag ttccatcgtc cgaaggccgc gaacaggagc ccgatgccgc caagcaaggc
     3961 gatcagggat agaacgctcc acaccacgac gttggcggtc ggctcgttct cgaccagtgg
     4021 ctcgggcggc cagttgttgg tgtacgtgta atccaacccg ggccgcaggg tcgaagccgc
     4081 ccacgaagtc cacgcgaag
//
LOCUS       DATAIR010000716         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_351556, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000716 DATAIR010000000
VERSION     DATAIR010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351556"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VHM69_17045"
     CDS             <1..184
                     /locus_tag="VHM69_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742148.1"
                     /translation="GFQRVTEADPASKFHFYEDEEFVFAQYQVELTVARPVVALDELV
                     AAAGQVPQREVLAPRS"
     gene            complement(196..810)
                     /locus_tag="VHM69_17050"
     CDS             complement(196..810)
                     /locus_tag="VHM69_17050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2742149.1"
                     /translation="MSGTSPAEESSLYAFDAAWVLGRRGPLAGADEAGRGALAGPLVA
                     AAVVLGEGEISWINDSKLLRPEQRERIFCALLRRAEAVSIVAFPAWWIDRHGLGRANR
                     EALLQALSSLNARAGCSLADGKLALGPSVECLPRADSKSAAVAAASVVAKVVRDDAMR
                     ALSEHHTGYGFERNRGYGTPQHRRALVQLGPCRVHRLSYAGVGD"
     gene            complement(824..1150)
                     /gene="rplS"
                     /locus_tag="VHM69_17055"
     CDS             complement(824..1150)
                     /gene="rplS"
                     /locus_tag="VHM69_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2742150.1"
                     /translation="MITSENLQQRDVTFKPGDTVRVHYRVIEGNRERIQVFEGLCISR
                     RGGGINETFTVRKNSFGVNVERIFPLHSPKIAKIEVATRGAVRRAKLYYIRDKVGKKA
                     RVKKAK"
     gene            complement(1147..>1750)
                     /locus_tag="VHM69_17060"
     CDS             complement(1147..>1750)
                     /locus_tag="VHM69_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742151.1"
                     /translation="SPDGRIDDMPYGGGAGMVVRVDVVARALEEVYGVPAKEVKAERR
                     VVLTEPAGRRLTHGYVEEVASGRDLSIICGRYGGMDERVWMELATEAVSLGDFVLSGG
                     EIVAAALADAAIRRLQGVLGNAESLVGESFSAEDVVGPPTYTRPAVWDGEEVPGVLLS
                     GDHAQIRAWRESEARERTRARTTGKRSPGHPEIHPREEKA"
BASE COUNT          287 a          623 c          522 g          318 t
ORIGIN      
        1 cggcttccag agggtgaccg aagccgatcc cgcgtcgaag tttcacttct acgaagacga
       61 ggagttcgtc tttgcacagt atcaggtcga gctcaccgta gcgcgtcctg tagttgcgct
      121 cgacgagctt gtagccgcgg ctggtcaggt accgcagcgc gaggtcctcg ccccacgctc
      181 ctgaggactt cttgttcagt ccccgactcc ggcgtagctg aggcggtgaa cccggcaggg
      241 gccaagttgc acaagggccc tccgatgttg cggagtaccg tagccgcggt tgcgctcgaa
      301 gccatagccg gtgtggtgtt cggagagggc gcgcatcgca tcgtcgcgga caaccttcgc
      361 cacgacgctg gcggcggcga ccgccgcgct cttcgagtcg gcccttggca ggcactcaac
      421 actggggccg agcgccagtt tgccgtcggc caggctgcac ccggctcggg cattcagtga
      481 tgaaagtgcc tggagcaatg cctcgcggtt cgcccttccg aggccgtggc ggtcgatcca
      541 ccatgcggga aaagccacta ttgagacggc ctcggcccgg cgtagcagcg cgcagaagat
      601 cctctcgcgc tgttccgggc gaagcaactt ggagtcgttg atccaggaga tctcaccttc
      661 gccgaggacg acggcagccg caacgagtgg ccccgccaac gcgccgcgac ctgcctcgtc
      721 agccccggcg agcggccccc gacggcccag gacccatgca gcgtcgaagg cgtagaggga
      781 actctcctcc gcgggagaag tccctgacaa cgcaagtgcc ggcctacttg gccttcttga
      841 ccctggcctt cttgccgacc ttgtcacgga tatagtagag cttcgcgcgc cgcaccgcgc
      901 ctcgggtggc cacttcgatc ttggctatct tcggggagtg aagagggaag atcctctcga
      961 cgttcacccc gaaggagttc ttccttacgg tgaacgtctc gttgatgccg ccgcccctgc
     1021 gcgagataca tagcccctcg aagacctgta tccgctctct atttccctcg atgacgcggt
     1081 agtggacccg cacggtgtcg cccggcttga acgtgacgtc ccgctgctgc aaattctcgc
     1141 tcgttatcat gccttctcct ctcgtgggtg tatctccgga tggccgggcg acctctttcc
     1201 ggtcgtcctt gcccgcgtac gctctcgcgc ctcgctctcg cgccaggcgc gtatctgcgc
     1261 atgatcgccc gataatagca cgcctggcac ctcctctcca tcccagacgg ctggcctcgt
     1321 atacgtggga gggccaacaa catcctccgc cgagaacgac tcgccgacca ggctctccgc
     1381 gttcccgagg actccctgga ggcgccgtat cgcggcgtca gcgagcgccg cggcgacgat
     1441 ttcacccccc gaaagcacga agtcgcccag cgagacggcc tccgtcgcga gctccatcca
     1501 cacccgctcg tccatcccgc catagcgtcc acagatgatc gagagatccc gccccgatgc
     1561 cacctcctcc acgtaaccgt gcgtgagcct ccggcccgct ggctccgtga gaacgacgcg
     1621 acgttcggcc ttcacctcct tcgccggaac gccgtagacc tcctcgagcg cccgcgcgac
     1681 gacgtcgacc cgtacgacca ttccggcacc accaccgtaa ggcatgtcgt cgatgcggcc
     1741 gtccggggag
//
LOCUS       DATAIR010000717         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_163562, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000717 DATAIR010000000
VERSION     DATAIR010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163562"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHM69_17065"
     CDS             <1..235
                     /locus_tag="VHM69_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2742152.1"
                     /translation="SNKPLLGAVALTFALQLLAIYTPFFQRFLETEALPLADLAIAVA
                     LSTIIFWAVEIEKWLARRRNGPENAPRQQDSMR"
     gene            232..426
                     /locus_tag="VHM69_17070"
     CDS             232..426
                     /locus_tag="VHM69_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742153.1"
                     /translation="MRVPADCSALAATDAVYLAETLGARLYDFDSVEMLCAFHVGIHV
                     GEAVAELNKWSAAQGSRTRI"
     gene            494..1237
                     /locus_tag="VHM69_17075"
     CDS             494..1237
                     /locus_tag="VHM69_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX2742154.1"
                     /translation="MQLTDTVEVFLFALLTAVATGIGAIPFVFVKDLTRRWLGVSNAV
                     ASGLMLSASFGLVYEGVGYGLLRTLAGAVTGLAFIVLVRRLLQRDEHPAVFAQMTDLD
                     ASKALLIVGVMTMHSFTEGVGVGVSFGGGIELGLFITLAIAVHNIPEGLAISLVLVPR
                     GVAWSRAALWSIFSSLPQPLMAVPAFVFVETFKPLLPYGLGFAAGAMIWMVFSELVPD
                     ALEEASSNLVAITITLSVLAMVAFQVLIR"
     gene            complement(1260..2060)
                     /locus_tag="VHM69_17080"
     CDS             complement(1260..2060)
                     /locus_tag="VHM69_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2742155.1"
                     /translation="MSWVMFRKALKDLRWTVLWYAFGLVLYGLLILSFYPTVRENAEE
                     MQQLMGAFPKSVMDAFGANNMATLAGWVGGKYLNLMWPLIMSVFLIMAGAATVAREVE
                     RGTIELLLSVPESRTRLLAAKLAALLTGILVLVAATIGSIALGAALVDETLAFENLLA
                     LGAVLLCFSVAVAGYTVLFSSFSKERSKPAGMAAGLTLAFYLAWVVSDVSENWEWLRN
                     VSIFTAYDPQRALQSGHIEPWHLVALIAVGLVGSLVALAIFRRRDAIS"
     gene            complement(2057..3004)
                     /locus_tag="VHM69_17085"
     CDS             complement(2057..3004)
                     /locus_tag="VHM69_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742156.1"
                     /translation="MASVPIQVERLTRYYGKRRGVMDLNFEVQEGEIFGFLGPNGAGK
                     TTTIRQLMGLMRPTSGTARVFGLDCWHDSTKVKAMVGFLPGDIRLYEKMTGEEMLDFF
                     AAFRRVQHRRYRRELAERLGLDLGAKIKHMSKGNRQKLAVAQALMHDSPLLILDEPSS
                     GLDPLMQVEFVDLLREEQARGKTVFLSSHQLPEVERIAHRVAIIREGRLVAVEEVARL
                     KQLRERRMEIILHEPVPAERFSALDGVRIVSEAPGGKQIVLAVRGKLGPLLRVLSDMP
                     IDDLTFAPPDLESVFLHYYSDDEEPERTSESRPPLEVRG"
     gene            complement(3155..3697)
                     /locus_tag="VHM69_17090"
     CDS             complement(3155..3697)
                     /locus_tag="VHM69_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX2742157.1"
                     /translation="MKRTQELRILSEDHHHGLVQARRLRRMADETKGAEPVARGFLEF
                     WQKDTSIHFRKEEEVLLPVVARHGGDLSAEPLVKMLEDHALIRGLVMRLSDEVVGGDV
                     RPETLREIGDRLEAHIRLEERAVFPLIEESLSQRALTELAARLEVKEAGPMAEPWVPA
                     EALSYAPWPGPGDSEGGGYD"
     gene            complement(3719..>4054)
                     /locus_tag="VHM69_17095"
     CDS             complement(3719..>4054)
                     /locus_tag="VHM69_17095"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742158.1"
                     /translation="GTRLIKVFLHISKEYQLERIRRRLERPDKRWKFDPKDLEDRELW
                     ASYRQAYEIMLNHCSTEYAPWYVIPAHHKWFRSLLVSQILVDTLENMDLSYPQASFDP
                     EDFSARSLS"
BASE COUNT          750 a         1280 c         1220 g          804 t
ORIGIN      
        1 ctcgaacaag ccgctcctcg gagcggtcgc cctgacgttc gcactgcaac tgctggctat
       61 ctacaccccc ttcttccaga gattccttga gacggaggcg ctgccgctcg cggatctggc
      121 gatcgcagtg gcgctgagca ccatcatctt ctgggctgtg gagatcgaga agtggctggc
      181 tcgaaggagg aatggccccg agaatgcgcc tcggcagcag gattccatgc gatgagggtt
      241 cctgccgatt gctccgcgct cgctgccaca gacgccgtat acctcgctga gaccctcggg
      301 gcgcgactct atgacttcga ttcggtagag atgctgtgcg ccttccacgt gggaatccac
      361 gtcggcgagg ccgtggcgga actcaataaa tggagcgcgg ctcaaggctc gcgcacgagg
      421 atttagaacg cggtgttctc gcgcgcccgg cgcaagtccg ggctgaaaag agggaggccc
      481 taaagtagac ggtttgcagc tgactgacac agtcgaagtc tttctcttcg cgttgctgac
      541 ggcggtggcc acggggatag gggcgatacc gttcgtattc gtgaaggatc tcaccaggag
      601 gtggctcggg gtttccaacg ctgtggcctc ggggctaatg ctgtcggcga gcttcggcct
      661 ggtctacgag ggcgtgggat acggcttgct gcgcacgctg gcgggggcgg taaccggact
      721 cgcctttatc gtcctggtgc gaaggttgct tcagcgtgat gagcaccctg ctgtcttcgc
      781 ccagatgacg gacctggacg ccagcaaagc gctgctcata gtcggggtta tgaccatgca
      841 ctcgttcaca gaaggagttg gcgtcggggt ttcattcggc ggcgggatag aactgggcct
      901 gttcatcaca ttggccatag ccgtacacaa catccccgag ggactagcca tcagccttgt
      961 gctcgttcca aggggcgtgg cgtggagcag ggcggccctc tggagcatct tctcaagcct
     1021 gccgcaacca ctgatggctg ttccggcttt cgtcttcgtc gagaccttca agcccttgtt
     1081 accttacggg ctcgggttcg cggcgggtgc catgatctgg atggtcttct cagagctcgt
     1141 ccccgacgct ctcgaggagg cgtcaagcaa cctcgtggcg atcaccatca ccctctccgt
     1201 cctcgccatg gttgccttcc aggtgcttat ccgttgaggg ggctctgtcc gagtcccggc
     1261 taggagatag catcgcgcct ccggaagata gcaagcgcaa cgagagagcc gaccaggcca
     1321 acggctatca acgctaccag gtgccagggc tcgatgtggc cgctctggag cgcgcgttgc
     1381 ggatcgtagg cggtgaagat cgagacgttc cttagccact cccagttctc gctcacgtcg
     1441 gagaccaccc acgccaggta gaaagccagc gtaagccccg ccgccatccc cgccggcttg
     1501 ctacgttcct tggagaagga ggagaacagc accgtatatc ctgccactgc gacgctgaag
     1561 caaaggagaa cagcgccgag tgcaagcagg ttctcgaacg ccagcgtctc gtccacgagg
     1621 gccgctccga gcgcgatgga tccgatggtc gccgcaacaa gcaccaatat gcccgtgagc
     1681 aacgcggcga gcttcgccgc gaggaggcgg gtacggctct cggggacgga gaggagcaac
     1741 tcgatggtgc cgcgctccac ctcgcgagcc actgtcgcgg caccagccat gatcaagaag
     1801 acgctcatga tcaggggcca catcaagttc aggtacttgc ctcccaccca ccccgccagg
     1861 gtcgccatgt tattggctcc gaaggcatcc atcacggatt tcgggaaggc tcccatgagc
     1921 tgctgcatct cctcggcgtt ctcgcgcacc gtggggtaga acgagaggat caggagcccg
     1981 tacaggacca ggccgaacgc gtaccagaga accgtccagc gcagatcctt gagcgccttc
     2041 ctgaacatta cccaactcat cctcgcacct ccaacggcgg ccggctctcg gacgtacgct
     2101 cgggttcctc atcgtcgctg tagtagtgga ggaacacgct ctcaagatcc ggtggcgcga
     2161 aggtaaggtc gtcgatcggc atgtcgctca gcacgcgcag gagcgggccg agcttgccgc
     2221 gcaccgcgag cacgatctgc ttgcctcccg gcgcctccga gactatccgt accccgtcaa
     2281 gcgccgagaa ccgctccgcc ggaacgggtt cgtgcagaat gatctccatc cgccgctccc
     2341 tcaactgctt gaggcgcgcc acctcctcga cggccacgag cctgccttcc ctgatgatgg
     2401 ccacgcggtg ggcgatgcgc tcgacctcgg gaagctggtg tgaggagagg aagacggtct
     2461 tgccgcgcgc ctgctcttcg cgcaacaggt cgacgaactc gacctgcatg agcgggtcga
     2521 ggccgctgct cggctcatcg aggatcaaga gcggagaatc gtgcatcaac gcctgggcga
     2581 ccgcgagctt ctgcctgttt cccttggaca tgtgtttgat cttcgcgccg aggtcaagcc
     2641 cgagacgttc ggccagctcc cgccggtaac gcctgtgctg taccctgcgg aacgccgcga
     2701 agaagtcgag catctcctcg ccggtcatct tctcgtagag gcggatgtcg ccaggtagga
     2761 acccgaccat cgccttcacc tttgtcgagt catgccagca gtcaaggccg aagacgcgag
     2821 cggtgccgct cgtcggacgc atcaggccca tgagctggcg gatggtcgtc gtcttgccag
     2881 cgccgttagg cccgagaaag ccgaagatct caccctcctg cacctcgaag ttaaggtcca
     2941 tgacgccacg tcgcttgccg tagtaacggg tcagccgttc gacctgtata gggacgctag
     3001 ccacgatcgt gctccttacc gctcatccat gtggctaatt taacgggcag acgaccgaac
     3061 ggcctgagca ctttgaatca ttcatctggt caaaatgagt caagagatcc tcgcttgtct
     3121 ccctcgcacc agagtcggag atctatccgg ctacctagtc gtagccgcca ccctcgctgt
     3181 caccgggacc gggccacggc gcgtacgaga gtgcttccgc agggacccaa ggctcggcca
     3241 tgggccctgc ttccttgacc tctagcctgg ccgcgagttc cgtcaaggcg cgttgtgaaa
     3301 gtgactcctc gatcagggga aacacggccc gctcctccag acggatgtgc gcctcgaggc
     3361 gatctccgat ctcacggagt gtctccggcc ggacgtcccc acccacgacc tcgtcgctca
     3421 gacgcatgac gagcccccgg atgagcgcat ggtcttcgag catctttacg aggggttccg
     3481 cggacaagtc tcctccgtgc cgcgccacca caggaaggag gacctcctct tccttgcgga
     3541 agtggatcga cgtgtccttc tgccagaatt ccaggaatcc tctggccaca ggttctgccc
     3601 ccttcgtctc gtctgccata cgcctgagcc tgcgggcctg cacaagaccg tggtggtggt
     3661 cctccgaaag aatcctcagt tcctgtgtac gcttcatcgg gtctcccttc cacgctcttc
     3721 aggagaggct tcgtgctgag aagtcctcag ggtcgaacga agcttgcgga tagctgaggt
     3781 ccatattctc gagtgtatcg acgaggatct ggctgactag aaggctacgg aaccatttgt
     3841 ggtgggctgg tatcacgtac catggcgcat actccgtcga gcaatgattg agcatgatct
     3901 cgtatgcctg ccggtatgaa gcccagagct ccctgtcttc gaggtctttc ggatcgaact
     3961 tccaacgctt atccggcctc tccagcctgc gccttattct ttccagctgg tattccttgg
     4021 agatatgcag gaataccttg atcagacgcg tgcc
//
LOCUS       DATAIR010000718         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_259824, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000718 DATAIR010000000
VERSION     DATAIR010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259824"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VHM69_17100"
     CDS             complement(<1..325)
                     /locus_tag="VHM69_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2742159.1"
                     /translation="MAEKVGFIGLGIMGKPMAKNLLDAGYDLVVYNRTREKAEELASE
                     GATVAGSPKEVAEQSDVIITMLPDSPQVEEVLTGEDGVLEGVSDSSLIVDMSTISPVV
                     TEELSE"
     gene            complement(339..1145)
                     /locus_tag="VHM69_17105"
     CDS             complement(339..1145)
                     /locus_tag="VHM69_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2742160.1"
                     /translation="MRFSANVSILFKEVPFLDRFGRAADSGFSAVEFWWPSDEEPGEV
                     ERAVKDSGLEVALFNFDAGDMPAGDRGLVGDPGREQQFRENVPVALDLARALECRRMN
                     VLVGHEVPGMDREEQLALARKNVRFAADKAGAVDVMVEAVNTFENGPYLLYTTEQAVE
                     FVKSVGRDNVKIQHDLYHMQRMEGNLVANLREYIGVIGHVQIADSPGRGEPGTGEIHY
                     PYVLAALEDLGYSGYVGLEYNPTTETTEESFGWLPEELRGGDVAVSDLKL"
     gene            1215..2504
                     /locus_tag="VHM69_17110"
     CDS             1215..2504
                     /locus_tag="VHM69_17110"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX2742161.1"
                     /translation="MRKDLQDIFSAGLAAADPAEAVRRSLRVEGGSITAVGRSFEAES
                     VSVVAAGKAAGAMARAAEEVLEGRISGGLVVTKEDHDPGPESFETVFASHPEPDERGV
                     EAARKVQELAESLGEGDLLLALISGGASALLADPAPPIELADLKKLTGDLLRSGADIG
                     EINTVRKHVSVLKGGGLVRLAHPAPTFALLLSDVVGDDPSSIASGLTAPDPTTLDDTR
                     RVLDRYEIEPPESIAAHLRSVEETPASDDPVFEDVVNVICGGGRHAAESASKKAEALG
                     YTPLLLTTVLTGDALGAASMYAAIVREVMASGNPIRPPCAIVSGGEATVTVRGNGTGG
                     PNQEFALTLAVELEGVEGWAAFAADTDGNDGSTDAAGGIVDGETAGKIRDSGVDPSEA
                     LADNDSYPALEAGNALLVTGPTGTNVNDLRVALVSPR"
     gene            2785..>3824
                     /gene="gltX"
                     /locus_tag="VHM69_17115"
     CDS             2785..>3824
                     /gene="gltX"
                     /locus_tag="VHM69_17115"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2742162.1"
                     /translation="MVNKEIRVRYAPSPTGMLHVGGVRTALFNWLFARKNGGIFVLRI
                     EDTDLERSTEESVEQLKRSMRWIGLDWDEGPEAGGPHPPYRQTERFELYREAARKLLD
                     SGAAYYDFATPEELAELRERAQAEKRQPIYTGGEYREMDPEEAKKRIEAGESYTVRFK
                     TPREGQTVVQDMIRGPVTFENANLEDFVLMKSTDTPTYNFAAAFDDAEMEISHVIRGD
                     DHLSNTPRQILIHEALGNPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAEKGYLPEA
                     LFNYLALLGAGYAADEEIFTPEELAARFRIDRVSGNPAILDEKKLRSVNALYLRRKSP
                     EELAALAAPMLV"
BASE COUNT          703 a         1267 c         1196 g          658 t
ORIGIN      
        1 tctcagatag ctcttcagtg accacgggag agatcgtgct catgtcaacg atcagggagc
       61 tgtcacttac tccttcgagt acaccatcct ccccggtaag cacctcttcg acctgcggcg
      121 agtcagggag catggtgatg atgacgtctg attgctcggc gacttctttc gggctgccag
      181 cgaccgttgc tccttctgag gcgagttcct cggccttctc gcgggtgcgg ttgtaaacga
      241 cgagatcgta gcccgcatcg aggaggttct tcgccatcgg tttgcccatg atcccaagcc
      301 ctatgaatcc taccttctca gccatcgtgc ctccctttct agagtttcag gtcagagacg
      361 gccacgtccc cgcccctgag ttcctcgggc agccagccga agctctcctc ggtcgtctcc
      421 gtcgtcgggt tatattcgag gccgacgtag cctgagtagc cgaggtcttc cagggcggcg
      481 aggacgtagg ggtagtggat ctcccccgtt ccaggctcac cgcgacctgg ggagtcggcg
      541 atctgtacgt gcccgatcac cccgatgtac tctcgcaggt ttgcgaccag gttcccctcc
      601 atcctctgca tatgatagag gtcgtgctgg atctttacat tgtcgcgccc gacgctcttc
      661 acgaactcga ctgcctgctc cgtcgtgtac agaagatagg gtccgttctc gaaggtgttg
      721 acggcctcga ccatgacgtc caccgctccg gccttgtctg cggcgaagcg cacgttcttg
      781 cgtgccagcg ccagttgctc ttcgcggtcc atgccaggga cctcgtgccc gacgagcacg
      841 ttcatcctgc ggcactccag agcccgcgcg agatccaggg ccaccggaac gttctccctg
      901 aattgttgct ctcgaccggg atctcccacc aggcccctgt ccccggcggg catgtctccc
      961 gcgtcgaagt tgaagagagc gacctcgagt ccagagtctt tgactgcgcg ctccacctcg
     1021 cccggctcct cgtccgacgg ccaccagaac tcgacggccg agaaccccga gtcggcggcc
     1081 cttccgaacc tatccaggaa cgggacctct ttgaacagga tggaaacgtt cgcgctgaac
     1141 ctcataccaa ccctccttcg actacaagca tacccaaagc gggcgccagc ttcccaaaca
     1201 gctagaatga tcccgtgcgg aaggacctcc aggatatatt ctcggccggc ctggccgcag
     1261 ccgaccccgc cgaagcggtc cgccgctcgc tgcgtgtgga gggcgggtcg atcaccgctg
     1321 tcgggcggag cttcgaggct gaaagcgtat ccgtcgtcgc ggcgggcaag gcagcggggg
     1381 cgatggcgcg ggccgccgag gaggttctgg aagggaggat ctccggcgga ctcgtggtca
     1441 ccaaagaaga tcacgacccc ggtcccgaga gtttcgagac cgtcttcgcc tctcatcccg
     1501 agccggacga aaggggcgtg gaggcggcac gcaaagttca ggagttggca gaatcgctgg
     1561 gagagggaga tctgctgctc gcgcttatct cgggcggggc ctcggcactg ctcgctgacc
     1621 ccgcgccgcc catcgagctg gccgacctga agaagctcac gggagacctg ctcaggagcg
     1681 gcgcggacat aggggagatc aacaccgtaa ggaagcacgt ctcggtattg aagggcggcg
     1741 gtctagtgcg gctcgcccac cctgccccga ccttcgccct cctgctctcc gacgtggtcg
     1801 gcgacgatcc ctcctccatc gcgagcggcc tcacggcccc cgacccgacg accctggatg
     1861 acaccaggcg cgtgctcgac cgctacgaaa tagagccgcc tgagagcatc gctgcgcacc
     1921 tgcggagcgt ggaggagacc ccggcatcag atgaccccgt cttcgaggac gtcgtgaacg
     1981 tcatctgcgg cggcggcagg cacgcggcag agtcggcgtc gaagaaggcc gaagcactcg
     2041 gatatacgcc gctgctgctc acaaccgtcc tcacgggcga cgccctcggg gcggcctcga
     2101 tgtacgccgc catcgtcagg gaggtcatgg cgagcggcaa cccgattcgt ccgccgtgtg
     2161 ccatcgtctc gggcggcgag gcgacggtaa ccgtgcgcgg gaatggcacg ggcggcccca
     2221 accaggagtt cgccctgacg ctggcggtgg agctcgaagg cgtcgagggc tgggcggcgt
     2281 tcgcggcgga cacggacggc aacgacggct cgaccgacgc ggccggcggc atcgtggacg
     2341 gggaaacggc ggggaagatc agggacagtg gggtagaccc gtcggaggcg ttggcggaca
     2401 acgactcata cccggcgctg gaggcgggca atgctctact cgtcaccggg ccgacaggca
     2461 ccaacgtcaa cgacctgcgg gtggccctgg tctccccgcg ctgaaggggc atctcaggac
     2521 cttcggcgac ctcggacttc acgacttgcc gagtccggcc cgcgcaccat tgagatgcta
     2581 gtcgttggtt gagacaggcg tgatttaccg gtgcctttag caacgtacac gaagccggca
     2641 caatgagacc gtagacgaag ttgtccacca gactcattag catcggcggg tctcatgagc
     2701 ctggcccgtc tcccaccata ctgaatacgc cagttcgccg ccggcaagct ttttaacccg
     2761 cgggccttgt gttaggttta ggtcatggtg aacaaggaga tcagagtacg atacgcgccg
     2821 agtcctaccg gcatgctcca cgtcggtggt gtgcgtacgg cgctcttcaa ctggctgttt
     2881 gcgcgcaaga acggcggcat ttttgtcttg cggatagagg atacggacct tgagcgctcg
     2941 acggaagagt cggtcgagca gctcaagcgg tctatgcgtt ggatcggcct tgactgggac
     3001 gagggacccg aggccggagg accccatccc ccgtaccgcc agaccgagcg cttcgaactc
     3061 taccgcgagg ccgcaaggaa actcctcgac tcgggcgccg cctactacga cttcgccacc
     3121 cctgaggagc ttgctgaact ccgggagcgg gcgcaggccg agaagcggca gccgatctac
     3181 accggcggcg agtaccgcga gatggacccg gaggaagcga agaagaggat agaggctggc
     3241 gagtcgtaca ccgttcgctt caagaccccg cgcgagggac agacggtggt gcaggacatg
     3301 atccgtggcc ccgtaacctt cgagaacgcc aacctggaag acttcgtcct catgaagtcc
     3361 acggacaccc cgacctacaa cttcgccgcc gccttcgacg acgctgagat ggagataagc
     3421 cacgtcatcc gtggcgacga ccacctctcg aacacgccgc ggcagatcct gatccacgag
     3481 gctctcggca acccgctccc cgccttcgcc cacgtccccc aggtcctcgg cccggacaag
     3541 aagaaactct ccaagcgcca cggggcggcg agcgtcgagg acttcgcgga gaagggctac
     3601 cttcccgagg cgctcttcaa ctacctcgcg ttgctcggtg ccggctacgc cgcggacgag
     3661 gagatcttca cccccgagga actggccgca cgcttccgca tcgaccgcgt gagcggcaac
     3721 cctgcgatcc tcgacgagaa gaagctcagg tccgtgaacg ccctctacct gcggcgcaag
     3781 agccccgagg aactggccgc cctcgccgcc cccatgctcg ttga
//
LOCUS       DATAIR010000719         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_163799, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000719 DATAIR010000000
VERSION     DATAIR010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163799"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VHM69_17120"
     CDS             complement(<1..1464)
                     /locus_tag="VHM69_17120"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HEX2742163.1"
                     /translation="MKGRAPGPWFEAGVGLSAGASFAVLGPVRDVFSTLPGVLMAATL
                     ILFLVPGALLTRWFFAEYFSGAALPPAAFVISTGIFALIGVPMLVWERSLAVYIWACG
                     LTVGVCVLAALAVLVWALRGGQRPPGPGSVVTDSGGIVWVPFAALVAVLAYIARITSP
                     SYSGDIWIYLSWVREYLGGDRLASVEPFFGGDVGLSRAKINGWLLEQAAASKVSGIDP
                     VHFVFSYLNPTLVVVAFLVFFALARILFKSDRMAFFSGCLYALFFAIHLSQSRIALGG
                     EFIQRLPEDKLAAKFLFLPLALAFAVAFMEGGRKRYFCGFAFLCCAVMAIHPIGLAIT
                     GISMAGFAILHLASNPRSRVAWARISAMGLAGLLVVAVPAVLVSFFTDEPLANALADS
                     DINSGDPDVLRNMIFVSPERNRIFEFANGSYMMHPSLLLDEVIAAAFLIGLPFLLWRL
                     RRSLAAQLLFGMMYLTSVVVYVPPIATFLGDEVVLPGQ"
BASE COUNT          369 a          493 c          504 g          212 t
ORIGIN      
        1 ctggccggga aggaccacct cgtccccgag gaacgtggct atcgggggca cgtacacgac
       61 gaccgaagtc aggtacatca ttccgaacag caactgggcc gcgaggctgc gtctaagccg
      121 ccagagcaag aatggtagtc cgatcaggaa cgcggcggcg atcacttcgt cgagcagaag
      181 cgaagggtgc atcatgtacg agccgttcgc aaactcgaag atgcggttcc tctccgggct
      241 cacgaagatc atgttcctca acacgtcggg gtcgcccgag ttgatgtccg agtcggccag
      301 ggcgttggcc agcggctcat ctgtgaaaaa agagacgagg acagccggaa cggcgacgac
      361 gaggaggccg gcgagaccca tggccgagat tctggcccaa gccacacgac ttcgggggtt
      421 ggacgccagg tgcagtatcg cgaatcccgc catggagata cccgtgatcg ccagtcctat
      481 cgggtggatc gccatgacgg cgcagcacag gaacgcgaag ccgcagaagt accgtttcct
      541 tccaccttcc atgaaggcga cggcgaaggc gagggcaagg ggtaggaaga ggaacttggc
      601 cgcgagctta tcctcgggca ggcgctggat gaattcgcct cccaacgcga tgcgcgactg
      661 gctgaggtgg atggcgaaaa agagcgcgta gaggcaaccg gagaagaagg ccatcctatc
      721 gctcttgaaa agtatgcgcg ccagggcgaa gaaaacgagg aaggccacca caaccagtgt
      781 cgggttcagg tacgagaaaa caaagtgcac ggggtcgatc ccggagacct tcgaggccgc
      841 cgcctgctcc agcagccagc cgttgatctt ggcgcgggag aggcccacat cacccccgaa
      901 aaacggttca acggaggcaa gcctgtcccc accgaggtac tcccttaccc aggaaaggta
      961 gatccagatg tcccctgaat agctgggaga tgtgatcctg gcgatgtagg cgaggacggc
     1021 gacaagggcc gcgaaaggca cccacactat tcccccgcta tcggtgacga ctgagcccgg
     1081 ccctgggggc ctctgcccac ctcgcagcgc ccagacgagc acggcgagcg cggccagaac
     1141 gcatactccg accgtcaggc cacaagccca gatgtagacc gccagcgaac gttcccagac
     1201 gagcatgggc acgccgatca gggcgaaaat gccggtgctt atgacgaacg ccgccggcgg
     1261 caatgcggcc cccgagaaat attcggcgaa aaaccaccgc gtcagcaacg cccccggcac
     1321 caggaacagg atcagggtcg ccgccatcaa gactccaggc aacgtcgaga agacgtcgcg
     1381 cacgggaccg agtaccgcga aggaagcacc cgcggagagt cccacgccgg cctcgaacca
     1441 tgggcccgga gcccgcccct tcactatccc ggtactccgc agatcaagct ccgcgagcga
     1501 ggacgcgaca gcttgccttc cgggcgcgcg gaaacgctca tgcagtccgt ttcgggcgtg
     1561 acctcgtcgc cgaagagg
//
LOCUS       DATAIR010000720         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_352378, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000720 DATAIR010000000
VERSION     DATAIR010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352378"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VHM69_17125"
     CDS             <1..303
                     /locus_tag="VHM69_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742164.1"
                     /translation="VGVMYLGVMVEMAPSEELYRNPLHPYTQALLSAVPVPDPDVELE
                     RERIVLEGDVPSPVNPPSGCRFRTRCPYATEACGREDPVWREVSPEHWVAACHCIS"
     gene            384..1244
                     /locus_tag="VHM69_17130"
     CDS             384..1244
                     /locus_tag="VHM69_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX2742165.1"
                     /translation="MRKIVATFSIVAFDPETGSLGVAVQSKFLAVGSVVPWARAGVGA
                     VATQAMANYNYGPRGLDLMSRHRTAGQTVEALISADEDREHRQLGVVDGRGRAATFTG
                     SECFEWAGGTTGEHYAAQGNMLAGRETVEAMARTYEVTDGDFATRLLSALDAGQEAGG
                     DARGKQSAALLVVREGGGYGGDNERAVDLRVDDHPDPIRELFRIRDLHTLYFGETRPE
                     DVVAVDGDVRRDVADALRRGGYIQGRDADDDAFLDALSAYIRTENFEEREQERGYLDR
                     AVLEYMKQQG"
     gene            1327..>2331
                     /locus_tag="VHM69_17135"
     CDS             1327..>2331
                     /locus_tag="VHM69_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742166.1"
                     /translation="MLEVNNLKTHFRTQDGVVKAVDGVSFSLEAGETLGIVGESGSGK
                     SVTALSIMQLNPKPPAEYPEGEILFEGQDLLKTSDKRMQEIRGNDIAMIFQDPMTSLN
                     PVFTVGNQIREAIRIHQNLSKSEAQEKTVQVLRDVGIANPERRATEYPHQFSGGMRQR
                     AMIAMGLSCNPKVLIADEPTTALDVTIQAQILELMVELQEKYGTAIIMITHDLGVVSQ
                     LADKVMVMYAGRAVEQATTDEVFYDPLMPYSWSLLRSLPRLDTAGEVRLLPIEGAPPS
                     LIFLPNGCNFSPRCPFVKDECREIDPRLEEKKPGHTAACILSVEEIEKNKHRVDEELG
                     V"
BASE COUNT          463 a          722 c          775 g          371 t
ORIGIN      
        1 gtgggggtga tgtacctcgg ggtgatggtg gagatggcgc cgagcgagga gctctacagg
       61 aacccgttgc acccgtacac ccaggccctg ctctccgcgg tccccgtacc cgaccccgac
      121 gtcgagctgg agcgcgagcg catagtcctc gaaggcgacg tgccctctcc tgtgaaccct
      181 ccttcgggtt gccgattcag gacgcgttgc ccttacgcca ccgaagcctg cggccgcgaa
      241 gacccggtgt ggcgcgaggt gagcccggag cactgggtcg ccgcgtgtca ctgcatcagc
      301 taacgcctcg tagacgattg ctccgtccac cgggtcccct tccagcgacg ctcggcaatg
      361 gacccatgac tagaaaggag cgcgtgagaa agatcgtcgc gaccttctcc atagtcgcct
      421 tcgacccgga gacaggctct ttgggcgtcg ccgttcagtc caaattcctg gccgtcgggt
      481 cggtcgtgcc gtgggcccgc gctggcgtcg gggccgtcgc cacccaggcg atggcgaact
      541 acaactacgg cccgcgcggc ctggatctga tgtccaggca caggaccgcc gggcaaacgg
      601 tggaggcttt gatctcagca gacgaagacc gcgagcacag gcagctcgga gtcgtggacg
      661 ggcggggcag agccgcgact ttcacggggt cagaatgctt cgagtgggcc gggggtacga
      721 cgggcgagca ctacgcggcc cagggcaaca tgctcgcagg cagggagacg gtcgaggcga
      781 tggcgaggac ctacgaagtg acggacgggg atttcgccac gcggctgctc tcggcgcttg
      841 acgcagggca ggaggcgggc ggggacgcgc ggggaaagca gtccgctgcg cttctcgtcg
      901 tcagggaggg agggggatac ggcggggaca acgaaagggc tgttgacctg cgtgtcgacg
      961 atcaccctga ccccataagg gagctattcc gcatcaggga cctccacacc ctctacttcg
     1021 gcgagaccag gcctgaagac gtcgtcgccg ttgacgggga cgttcgcagg gatgttgcag
     1081 acgcgctgag acgcggcggg tacattcagg ggcgggatgc cgacgacgat gcgtttctcg
     1141 atgcgctaag cgcctacatc cgcaccgaga acttcgagga gcgcgagcag gagcgcggct
     1201 atctcgaccg ggcggtactc gagtacatga agcagcaggg gtgaggctct ggccggaacg
     1261 ccgccactac tgtacccttc atttcggcgg aaaggccgca cggggagcga ggattaacct
     1321 gtgaccatgc tggaagtgaa caacctcaag acgcacttca ggacccagga cggggtcgtc
     1381 aaggctgtgg acggtgtctc gttctcgctc gaggcggggg agaccctcgg gatagtcggg
     1441 gagtccggga gcggcaagag cgtgacggcc ctctccatca tgcagctcaa ccccaagccc
     1501 ccggccgagt atcctgaggg tgagatcctc ttcgagggcc aggacctgct aaagacctcg
     1561 gacaagagga tgcaggagat caggggcaac gatatagcca tgatcttcca ggaccctatg
     1621 acgagcctca accctgtctt cacggtaggc aaccagatcc gcgaagccat ccgcatccac
     1681 cagaacctct ccaagagcga ggcccaggag aagaccgtgc aggtccttcg cgacgtcggc
     1741 atagcgaacc ccgaaaggag ggccacggag tacccgcacc agttctccgg cggcatgcgc
     1801 cagcgggcca tgatcgcgat gggcctctcg tgcaacccga aggtcctgat agccgacgag
     1861 ccgaccacgg ccctcgacgt gaccatccag gcccagatcc ttgagctcat ggtcgaactg
     1921 caggagaagt acggtactgc cataatcatg atcacccacg acctcggggt agtctcccag
     1981 ctcgcggaca aggtgatggt gatgtacgcg ggacgggccg tcgagcaggc aacgacggac
     2041 gaggtcttct acgatcccct gatgccctac tcgtggtccc tgttaagatc ccttccccgc
     2101 ctcgataccg ccggcgaggt gagactactg ccgatcgagg gtgcgcctcc gtccctgatc
     2161 ttccttccaa acggatgtaa cttcagccct cgctgcccgt tcgtgaagga cgaatgccgc
     2221 gagatcgatc ccaggctgga agagaagaag ccgggccaca ccgccgcttg catcctctcg
     2281 gtagaggaga tcgagaagaa caagcaccgc gtggacgagg agcttggggt c
//
LOCUS       DATAIR010000721         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_214703, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000721 DATAIR010000000
VERSION     DATAIR010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214703"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VHM69_17140"
     CDS             complement(<1..324)
                     /locus_tag="VHM69_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2742167.1"
                     /translation="MDHNDFAERVERIVGNVERAVSGKRDGVLLAVVAMLAGGHVLIE
                     DVPGVGKTLLAKSLARSLRAEFRRIQFTPDLLPADVTGLNVYNQGEGDFEFWPGPVFA
                     NVVLAD"
     gene            complement(503..1048)
                     /locus_tag="VHM69_17145"
     CDS             complement(503..1048)
                     /locus_tag="VHM69_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2742168.1"
                     /translation="MARCLTLNASYEPVALVTVKRAIVLVVSEKAEIVEANLDRRFRS
                     ERAEYPYPLVIRLVKYVEIPRRLRKHVTNAILFARDNYRCQYCSRHRGDLGRRDRLTR
                     DHVKPISRGGANTWDNVVTACTKCNARKGNRLPMECGMYPKSTPKEPRYVVMVWYSRD
                     LNEVQRRYVDQFYGQGSLTAS"
     gene            complement(1124..>1655)
                     /locus_tag="VHM69_17150"
     CDS             complement(1124..>1655)
                     /locus_tag="VHM69_17150"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2742169.1"
                     /translation="DAGLSPEFVPTGQTGIVIAGWGLCVDAVVSDFVPGASEQLVIEA
                     AKTSPDLILVEGQGSLGHPAYSGVTLGLMHGSCPDCLILCAADPSEDVFRGVPRPSPA
                     RVARLYEEVASLIKPAPVVAVSLNTRGMDEEKALDLIAAVADETGLPTADPFRGSAAP
                     ILEAVLQAPKSRAVGL"
BASE COUNT          322 a          523 c          505 g          305 t
ORIGIN      
        1 atccgcgagc accacgttgg cgaagacagg accgggccag aactcgaagt ccccctcgcc
       61 ctggttgtag acgttgaggc ccgtcacgtc ggcaggcaac aggtcgggtg tgaactgaat
      121 cctgcggaac tctgccctca aggagcgggc gagcgacttg gccagaagcg tcttgcccac
      181 accggggacg tcctctataa ggacgtgtcc tcccgcgagc atcgccacca cggcaagcaa
      241 gacaccatcg cgtttgccag aaacagcacg ctcgacgttc ccgacgatac gttcgaccct
      301 ctccgcgaaa tcgttatggt ccaaaggagt cctgtctatg ttcgcgtgac gcagggaatt
      361 atagctcata ggctacaggc ttcagatctt ctcagcgact cgtgatcatc gatggatcaa
      421 cggtcgtccc gcccacgacg gtacattatc gcggcagaac tggtcctcca acacctgaag
      481 cctgaaacct gttgcctgaa gcctacgaag ccgtcagact tccctgcccg tagaactgat
      541 ccacgtatcg gcgctggacc tcgttgaggt cgcgcgagta ccacaccatc acgacgtagc
      601 gcggctcttt gggcgtgctc ttggggtaca taccgcactc catcggcagt cggttgccct
      661 tcctcgcgtt gcacttggtg cacgccgtta ccacgttgtc ccaggtattc gccccgccgc
      721 gtgatatggg cttgacgtga tccctggtca ggcggtcgcg gcgcccgagg tcacccctgt
      781 gcctggagca atactggcaa cggtagttat cgcgggcgaa gaggatcgcg ttagtcacgt
      841 gtttgcgcaa gcgacggggt atctcgacgt acttgacgag cctgatgacg agggggtacg
      901 ggtactccgc cctctccgaa cggaagcggc gatccaggtt cgcctcgacg atctcggcct
      961 tttcggagac gacgaggacg atagccctct tgaccgtcac cagggccacg ggctcatagc
     1021 tcgcgttgag cgtcaagcat cgtgccacgc cgaaatctta acatacgcgg atgcttgcag
     1081 cgtggctcgc agcgtaaaat gctcgttaca cgaagcgcgc ctcttagaga cccacagccc
     1141 tgctcttcgg cgcctgcagc acggcctcca gaataggcgc ggccgaaccc ctgaagggat
     1201 ccgcggtcgg cagtcctgtc tcgtctgcta cggcggctat cagatccaga gctttctctt
     1261 cgtccatccc cctggtgttg aggctgacgg cgaccacggg ggccggcttg ataagggatg
     1321 cgacttcctc gtagaggcgg gccacgcggg ccggggaagg gcgcgggacg ccccggaaga
     1381 catcttcgga agggtcggcg gcgcacagga tgaggcaatc agggcatgac ccgtgcatga
     1441 ggccgagcgt aacccccgaa tacgcgggat gccccaggga gccctggccc tcgacaagga
     1501 tgaggtcggg cgaggttttg gccgcctcga tcacaagttg ctcggatgcc ccagggacga
     1561 agtccgagac cacggcgtcc acgcagaggc cccatcctgc gataactatt ccggtctgtc
     1621 ccgtagggac gaactccggg gagagaccgg catcc
//
LOCUS       DATAIR010000722         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_352671, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000722 DATAIR010000000
VERSION     DATAIR010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352671"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..584
                     /locus_tag="VHM69_17155"
     CDS             75..584
                     /locus_tag="VHM69_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2742170.1"
                     /translation="MTFGRFTNKSRRCVEAAFEEARMLGHDALGDEDLLLGILRADEG
                     IAVEALSSLGVTLEKAREESEEMLSDALSSIGISLEDIRREAGDAFDMRIPDNRRIPF
                     SPRAKKALVEARKLMRSLGDNYLGTEHVLLGMLRNDDGTAVRILARMGVSPEALEDRL
                     FELRGRAAS"
     gene            605..1324
                     /locus_tag="VHM69_17160"
     CDS             605..1324
                     /locus_tag="VHM69_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX2742171.1"
                     /translation="MGTVYMDEKTRTLLACGVIAGPLFVVTFLAEGATRAGYEPLRHP
                     VSSLALGEHGWMQTANFVVAGLLTLAFAVGMRRVSPKLSIRGPLLVGVWAIGLIGAGI
                     FPTDPVSGYPPGTPDKLTGYSWHGAIHDLAFSLPGFVALAAACFVFCRWFAGRGDRRW
                     AVYSALTGLVFSVSFILASAGFGQAEVLVHLAGLFQRVAVAVGFGWLTLLAVHSLRSS
                     PGGQPGSSRSEDPPAAGRVGR"
     gene            1334..1768
                     /locus_tag="VHM69_17165"
     CDS             1334..1768
                     /locus_tag="VHM69_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742172.1"
                     /translation="MEDREERGGAVRVGMVWGGIGGVVGLLVSLLGSLVGILVAGFIG
                     FSCGRRAAAAGEGSGALSGLVGGAVAAPVYVLGSSIGALVAGREIGAARIATTLSEML
                     GTSLSAEEAWTFFLFSLVLSAVLQIVILILSSTAAGAWTSRK"
     gene            complement(1773..2252)
                     /locus_tag="VHM69_17170"
     CDS             complement(1773..2252)
                     /locus_tag="VHM69_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX2742173.1"
                     /translation="MLDRQLQIQILTDHYQRPRHRGSIEDADVAMPGGNPGCGDVVTV
                     YLKGAEDQEHIEDATYEGEGCTISMAASSLLLEEVHREDMTMDQVLDMDYNEMIEQLG
                     RQIVASRPRCATLGLGTLKAAIRKYQKDRRLERAGVARPDDGEFVFGENAAEAARKE"
     gene            2343..>3268
                     /gene="ychF"
                     /locus_tag="VHM69_17175"
     CDS             2343..>3268
                     /gene="ychF"
                     /locus_tag="VHM69_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX2742174.1"
                     /translation="MRVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVASV
                     PDGRLDALADVMKSKRVVPTTVDFVDIAGLVRGASAGEGLGNQFLGHIRECEAIAHVV
                     RCFEDDNVVHVHAGVDPEGDIETIDTELLLADLATVERRLERVGRAAKSGDARLKAEA
                     AELEKLMDHLSDGSPARAYPESETLAEAMSTLITAKPTLYVANVDEESVAEGNGYSSK
                     VEKHAAREGAEAVRISAKLEAEVAELSEDEAVEYLAMLGVETTGFAEFVRAAYRLLGL
                     VTFFTAAEKESRAWTVHEGSTVRRAAGRIHTD"
BASE COUNT          542 a          989 c         1105 g          632 t
ORIGIN      
        1 caggcggtgc acaaaaagca cgccaggagg atcaaggaca agtaccctgg agaaccgccc
       61 gggaggaagc gcacatgacg tttggccgtt tcaccaacaa gtcccgcagg tgtgtggagg
      121 ctgccttcga agaggcgagg atgctgggtc acgacgcttt aggtgacgag gatctcttgt
      181 tggggatact gcgcgccgac gaggggatag cagtggaggc gctctcttcg ctgggcgtaa
      241 cactggaaaa ggcgcgggaa gaatctgagg agatgctttc agacgcgctc tcgtctatcg
      301 ggatctccct cgaagacatc cgcagggaag cgggggacgc cttcgatatg cgcatcccgg
      361 acaaccggcg catcccgttc tccccacgcg ccaagaaggc gcttgtggaa gcgcgaaaac
      421 tgatgcgaag tctcggggac aactacctcg gcaccgagca cgtcttgctc ggtatgctgc
      481 gcaacgatga cgggacggcc gtcaggattc tggccagaat gggcgtgtcg cctgaggcgc
      541 tggaagaccg gctcttcgaa ctgcgtggaa gggcggctag ctagcgccaa cgtggccggg
      601 agctgtgggc acggtctaca tggatgagaa gacgagaacg ctccttgctt gtggtgtgat
      661 cgccggtccg ctgttcgtcg tcaccttcct cgcagagggc gccacccgtg ccggctacga
      721 acctctgcgt catccggtca gttcgctcgc cctcggagaa catggttgga tgcagaccgc
      781 caacttcgtc gtcgcgggtc tcttgacgct ggccttcgcg gtcggcatgc ggcgcgtgtc
      841 ccccaagctt tcgatccggg gaccattgct cgtcggggtg tgggcgatcg gactgatcgg
      901 agcgggaatc ttcccgacag atccagtgag cggatatcct ccagggacgc ccgacaagct
      961 gaccgggtac agctggcacg gcgccatcca cgacctcgcc ttctcgctcc ccggctttgt
     1021 ggcgctggcg gcggcctgct tcgtgttctg ccgctggttt gccgggcggg gcgaccgcag
     1081 gtgggccgtc tattcggccc tcacgggtct cgtcttttcg gtctcgttca tcctcgccag
     1141 cgcgggattc gggcaggcag aagtcctcgt gcatctggcc gggcttttcc agcgcgtcgc
     1201 ggtcgcggtc ggattcggct ggctaacact tctcgccgtc cattcgctga gatcgtctcc
     1261 tggagggcaa cctgggagct ccagatctga ggaccctccg gccgccggtc gcgtgggtcg
     1321 ttagaatgag gctatggagg acagagaaga acgcggcggc gccgtccggg tcgggatggt
     1381 ctggggcggc attggtgggg tggttggcct ccttgtgtcg ttgcttgggt cgctggtcgg
     1441 gatcttggta gcgggcttca taggtttctc gtgcgggagg agggccgcgg ccgcgggaga
     1501 agggtcaggg gcgctctccg ggctcgtcgg cggtgccgta gccgcgcctg tttacgtgct
     1561 cgggtccagt atcggggcgc tggtggcggg acgtgagatc ggggctgcca ggatcgcaac
     1621 gaccctatcg gagatgctcg gtaccagtct ttcagccgag gaggcctgga ctttcttcct
     1681 cttcagcctc gtcctctcgg cggtccttca gatcgtgatc ttgatcctct cctcaacggc
     1741 cgccggtgcc tggacgagca ggaaatagaa ggctactctt ttctcgcggc ttccgccgcg
     1801 ttctcgccga agacgaactc cccgtcatcg gggcgcgcca cgccggctct ctccaggcgg
     1861 cggtccttct gatacttcct gatggcggcc ttcaacgtgc ctagacccag ggttgcgcat
     1921 ctcggacggg aggcgacgat ctggcgcccg agctgttcga tcatctcgtt gtagtccatg
     1981 tcgaggacct gatccatggt catgtcttcc ctgtggacct cttcgaggag caggctgctc
     2041 gccgccatgc tgatcgtgca gccctcgccc tcgtacgttg cgtcctctat gtgctcctgg
     2101 tcctcggctc ctttgagata cacggtaacc acgtccccgc atccgggatt gccgccgggc
     2161 atcgccacgt cggcgtcctc gatggagccc ctgtggcgcg gcctctggta gtggtccgtg
     2221 agtatctgaa tctgtagctg cctgtccaac acgcaacctt ccactctgtt gatggatact
     2281 ccaattctat cgcacactcc tcgccgtcac gtaaacttgc tacagtttga tacactcacc
     2341 ccatgagagt aggaatagtc gggctgccca acgtcggtaa gtccacggta ttcaacagcc
     2401 ttacacgggc gggggccgag gcccagaact atccgttcac gaccatcgac ccgaacctcg
     2461 gcgtcgcctc ggtaccggac gggcgtctgg atgcgcttgc tgatgtgatg aagagcaagc
     2521 gcgtcgtccc cacgacggtc gacttcgtgg atatcgccgg actggtgcgg ggcgcgagcg
     2581 cgggggaggg gctaggcaac cagttcctcg ggcacatcag agagtgcgaa gccatagcgc
     2641 acgtcgttcg ctgcttcgag gacgataacg tggtgcacgt acatgctggc gtcgaccccg
     2701 agggcgacat cgagacaatc gacacggagt tgctgctggc cgacctcgcg accgtcgagc
     2761 ggcggctgga gcgggtcggg cgggccgcca agagcggcga cgccagactc aaggccgagg
     2821 cggctgagct cgagaagctc atggaccatc tctccgacgg aagtccggcg cgggcttacc
     2881 ccgagtccga gacgctagcc gaggcgatga gcaccctgat cacggcgaag ccgacgctct
     2941 acgtggctaa cgtggacgag gagtccgtgg ctgaagggaa tggctacagt tccaaggtcg
     3001 agaagcacgc tgcgcgcgag ggtgctgagg cggtcaggat ctccgcgaaa ctcgaagcgg
     3061 aggtcgccga gctctccgag gacgaggccg tcgagtacct ggcgatgctc ggggtagaga
     3121 cgacgggatt tgcggagttc gtgcgcgccg cgtaccggct gctcgggctc gtcaccttct
     3181 tcaccgcggc ggagaaggag agcagggcct ggaccgtcca cgagggctct acggtgcggc
     3241 gggccgcggg gcgcatccac acggacat
//
LOCUS       DATAIR010000723         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_260713, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000723 DATAIR010000000
VERSION     DATAIR010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260713"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..799
                     /locus_tag="VHM69_17180"
     CDS             200..799
                     /locus_tag="VHM69_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2742175.1"
                     /translation="MEGDRRTNGEEEYAAGTTDDRGESRGDPDVQLDVPKLNVEEIDL
                     EVEDLQVRVAFLAELADLVKINVGLDAEVNEVKLQIKGVEAQAQLKARLDNVRAIFSE
                     VLTSLQHSPQFFREALGPVNQTTETPEETTWDTDVADESADTFAPSDDGAADEPVEPE
                     ATQAARKKAGDLGVDLSGVDGTGSGGRILVRDVTKAAKG"
     gene            850..1176
                     /locus_tag="VHM69_17185"
     CDS             850..1176
                     /locus_tag="VHM69_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742176.1"
                     /translation="MANKFFKQTSAEILSDLLSDARVKRVREYVQGEEFKADRERIRR
                     DAAERLKDIRERYRGKRRDPETAARERELTLRLAEVEAEAADLRIQLSELLEEEEKLR
                     SALDEL"
     gene            1519..>3647
                     /locus_tag="VHM69_17190"
     CDS             1519..>3647
                     /locus_tag="VHM69_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX2742177.1"
                     /translation="MTPRAGLYDPVYEHDACGLGFVARLDGRRTRETVEEGLEVLHNL
                     EHRGTTGSDPETGDGAGILTQIPDGFFRKECKELGIELPDAGHYGVGMLFEPGEGLGC
                     EEGLAEICAEEGHDLLGFRDVPIVPEAVGKVARSVMPRIRQFFIRRRGGDQAAFERKL
                     YVIRRRLHNAVQDKHGCYVVSLSSRTIIYKGLLKGQQLPRFYPDLGDPQFASAIALVH
                     ERFSTNTLGSWELAHPYRYIAHNGEINTIRGNVNWMRARESRLRSELFGEDLAKLSPV
                     IIPGQSDSAAFDNALELLHLAGRSLPHAVSMMIPEAWENDELMDPERRAFYQYHCALM
                     ESWDGPAAVAFTDGRLIGATLDRNGLRPARYSVTRDGRVIMASEDGALRVPAEDVIER
                     WRLQPGKMLVVDTERHELLHDEEVKKPLFGRQPYRQWLEEGEIHLNELPEPDADPRDE
                     PASLLERQRAFGYTIEDQRILLAPMAQNGKEPDGSMGTDTPLAVLSERPQLLFSYFKQ
                     HFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETPEHCRRVLIDQPVLTADELEKIR
                     RLGSERAATSTLSTLFSVSTGEEGMEEALGRLCEKAVSAVKGGSAVLVLSDRGVNETQ
                     APIPSLLATASVHHHLVRAGIRTATTLVVETAEAREVHHFALLVGYGATALNPYLAFE
                     TIEELAGSHLMEGVSPEEAAKNYVKAVG"
BASE COUNT          724 a         1129 c         1182 g          612 t
ORIGIN      
        1 gttccctttc gacgtccatc ctatgcatgt gtataccccg tacgcgtcga agtaagcacg
       61 gagcgtacct tagcgtgtgc cctggcaagt ttatttacgg ggtaggaggg gcatgacaga
      121 caaatagcga gaagttttgt agtggcgatc gtttatgtta tccagagggt ccgaagcgcg
      181 acgggaggag cgtccagcgg tggaaggtga caggcgcacg aacggggagg aagagtacgc
      241 cgccggcacc acggatgacc gcggggagag ccggggggac cccgacgtac agttggacgt
      301 acccaagctc aacgtcgagg agatcgacct cgaggtcgag gacctgcagg tccgtgtagc
      361 tttcctggcc gagcttgctg acctagtgaa gatcaacgtc ggcctcgacg ccgaggtaaa
      421 cgaagtgaag ctgcagatca aaggcgtcga agcccaggcc caattgaagg cccgcctgga
      481 caacgtgcgg gcgatcttca gcgaggtact gacctccctg caacacagcc cccagttctt
      541 ccgcgaggcg ttgggaccgg tgaatcagac gaccgaaact ccagaggaga cgacctggga
      601 caccgatgtc gcggatgaga gtgccgatac tttcgctcct tcggacgacg gggctgcgga
      661 cgaacccgtt gaaccggagg cgacgcaagc cgccaggaag aaggccgggg accttggcgt
      721 tgatctctca ggcgtcgatg gcacgggctc tggtggacgc atcctcgtca gggacgtcac
      781 gaaggccgca aagggataac cttacctccc ccagcgggta caatcaggag gagtggaagg
      841 agaagtcccg tggccaacaa gtttttcaag cagacctcgg ctgagatcct ctccgacctg
      901 ctctccgacg cgagggtgaa gcgtgtcaga gagtacgtgc agggcgagga gttcaaggcc
      961 gaccgcgaac gcatccgtcg cgacgccgca gagcgcctga aggacatccg cgaacgctac
     1021 cggggtaaac ggcgagatcc cgagaccgcc gcacgcgaga gggagctcac cctgcgcctc
     1081 gccgaggtag aggccgaggc cgccgacctg cgtatccagc tctccgagct gctcgaggaa
     1141 gaggagaagc tgcgcagcgc gctcgacgaa ctgtagtgcg gcacttcgtg acctcccgaa
     1201 gtagaggccg aggcttcagg catcaaaacg ctccggcctc cactctcagg tatcagctct
     1261 ttccggtttc atctgggctt accgctgact acgcccacca ccgcatctcg gtcgaaacgc
     1321 aggaccagcg actcccccgc cggcgcgggt ccggctcgcg tcaagatcct gaaacctgat
     1381 gcctgaagcc tgacacctca ttttccagag gccttaccag gtactagtat gcaacaggat
     1441 tgctacgcac ttgtgctttg cgtcgaattc gtgtaaaaat gggccttccg caccctggac
     1501 gccataggag gcgatttgtt gacccctaga gccggacttt acgaccctgt gtacgagcac
     1561 gatgcttgcg ggctcgggtt cgtagcgagg ctcgacgggc gcaggacgcg ggaaacggtc
     1621 gaggagggcc tcgaggtcct gcacaacctg gagcatcggg gcactacggg gagcgacccc
     1681 gagacggggg atggggcggg catactcacc cagatccccg acggcttctt ccgcaaagag
     1741 tgtaaggaac ttggcatcga gcttcctgac gccggacatt acggggttgg gatgctcttc
     1801 gagcctgggg agggcctcgg ctgcgaggag gggctcgctg agatctgtgc cgaggaaggc
     1861 catgaccttc ttggtttccg cgatgtcccc atagtgcccg aggcggtcgg gaaggtcgcg
     1921 cgcagcgtca tgccgcgcat caggcagttc tttatccggc ggcgcggagg agaccaggcc
     1981 gctttcgagc ggaagctcta cgtcatccgg cggcggctac acaacgcagt ccaggataag
     2041 cacggctgct acgtggtcag cctctcctcc aggacgatca tctacaaggg gctgttgaag
     2101 ggccagcagt tgccgcggtt ctatcccgac ctcggagacc ctcagtttgc gagtgcgatc
     2161 gcactcgtgc atgagcggtt ctcgacgaac actttaggga gctgggagct cgcgcacccc
     2221 taccgctaca tcgcgcacaa cggtgagatc aacaccataa ggggcaacgt taactggatg
     2281 cgcgcccgcg agagtcgcct acgctcggag ctgttcgggg aggacctcgc gaagctctcc
     2341 cctgtcatca tacctggcca gagcgactcg gccgccttcg acaacgccct tgagctgttg
     2401 cacctcgcgg gacgttccct gccgcacgcg gtctccatga tgatccccga ggcctgggag
     2461 aacgacgagc tgatggaccc cgagcgccgc gctttctacc agtaccactg cgccctcatg
     2521 gagtcgtggg acggcccagc cgcggtcgcc ttcaccgacg gtcggctcat cggcgcgacc
     2581 ctcgaccgga acggtttgcg tcctgcccgc tactccgtca ccagagacgg gcgggtcata
     2641 atggcctccg aggacggggc tttgagggtg cctgcggagg atgtgatcga acgctggagg
     2701 ctgcagccgg ggaagatgct cgtcgtcgat actgagcggc acgagctttt gcacgacgag
     2761 gaagtaaaga agcctctgtt cgggcggcaa ccctatcggc agtggctcga ggagggtgag
     2821 atccacctga acgagctccc cgagccggac gccgacccgc gcgacgagcc ggcctcgctc
     2881 ctggagcggc agcgggcctt cggctacacc atcgaggacc agcgcatact cctcgccccg
     2941 atggcccaga acggcaagga gccggacggt tcgatgggca cggacacgcc actggccgtc
     3001 ctctccgaga ggccgcagct cctgttctcg tacttcaagc agcatttcgc gcaggtcacc
     3061 aatcctccga tagatccact gcgcgaggag ctcgtcatgt cgctcaagat gagcctcggg
     3121 cccgagcaga acctgtttga cgagacgcct gagcactgcc ggcgcgtcct catagatcaa
     3181 cccgttctaa cggccgatga actcgagaag atccgacgcc tcggctccga aagggcagcc
     3241 accagcacgc tctcgaccct cttctccgtc tcgaccggcg aagaggggat ggaggaggcc
     3301 ctgggccgcc tgtgcgagaa ggccgtgagc gcggtgaagg gcgggtccgc ggtcctggtg
     3361 ttgagtgatc ggggcgtgaa cgagacgcag gcccctatac cgagcctcct tgctaccgcg
     3421 agcgttcatc accatctggt gcgggcaggt atcaggacgg ccacgacgct cgtcgtggag
     3481 actgcggagg cgcgggaggt ccaccacttc gccctcctcg tcggatacgg tgcgaccgcc
     3541 ctgaacccat acctcgcctt cgagaccata gaagagctcg cgggctcgca cctcatggag
     3601 ggcgtgagcc ccgaggaggc cgccaagaac tacgtcaagg ccgtggg
//
LOCUS       DATAIR010000724        12084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_71208, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000724 DATAIR010000000
VERSION     DATAIR010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71208"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VHM69_17195"
     CDS             <1..1476
                     /locus_tag="VHM69_17195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2742178.1"
                     /translation="VLERRDVIGGATVTEEPWPGYRLSTCSYVCSLLLPEVIRDLDLK
                     RHGYDVRALDPQYFVPFPDGRHLFSFLDGAKTKEQIRKFSEKDARSYDAYWAMWGRII
                     ARMRPLLTRSSPTVEEIERAFEGPQGEQDWDTLTRSSVAELLDEYFESEEVKAPLCVG
                     GVIGTNAGPRDPGTAYVKYHHIIGNLDGHQGAWGFVRGGMGAVAEAIALSAKAQGAEI
                     LTKAEVTEIEIQDGAARGVHLADGRSFEADVILSNADPHRTFLGMVGEKHLPPELVDG
                     VENARTKGSVVKVLLALGELPDFTALPGRKVGPQHTGAVVINPSIDYLQRAWEDCEQG
                     EPSEKPFMEGYIQSATESGLAPTGKHTMSLFCQYAPYELDEGTWEHRRAGIGANIIET
                     FAEYAPNLPDAIEHIEVLGPPDIEEKIGITGGNIFHGEILPDKLFGGRPVPGYSGYRT
                     PIENLYLCGAGTWPGGAVFGAPGRNCAMEVLAGQHERLALR"
     gene            1502..2935
                     /locus_tag="VHM69_17200"
     CDS             1502..2935
                     /locus_tag="VHM69_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX2742179.1"
                     /translation="MQTETKRVKNFVGGEHVDPADGRFYDLVNPSTGETFAQAPASGK
                     GDVDRAFEAAAKAFESWRDTTPSERQLALLRLADAIEERGEELVRAESENTGKPFGLT
                     MEEEIPPMADQIRFFAGAARILEGKSAGEYLEGYTSFIRREPVGVIGQVTPWNYPMMM
                     AVWKFAPAIAAGNTVVLKPSDTTPVTTVMLAEIAAEFLPDGVFNVVCGDRDTGRALVE
                     HPIPQMVSITGSVRAGMQVAEAAARELKRTHLELGGKAPVIVFDDADLEEAAEAIAGA
                     GYFNAGQDCTAATRVLAAPGIHDDFVAAITEQAKNTSTGSPDEDVDYGPLNNPTQLEH
                     VSGMVERLPDHAEVTTGGEKVDGSGYFYSPTVVSGLRQDDEASQTEIFGPVVTVQSFS
                     NEDEAVRWANGVKYGLASSVFTRDHARAMRVSRRLDFGCVWINTHIPLVAEMPHGGFK
                     HSGHGKDLSMYGLEDYTRIKHVMTSLG"
     gene            complement(2967..3620)
                     /locus_tag="VHM69_17205"
     CDS             complement(2967..3620)
                     /locus_tag="VHM69_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742180.1"
                     /translation="MEPIRVLISDDHPHFRDGMRALLLSAPDVEVVGEAGDGEKTIRL
                     AEKLQPDVILMDLNMPGISGIEATRRILHTSPHISVLVISMYEDDDSVFAALKAGARG
                     YLLKGALKAEILRSIRAVVSGEAIFGPAIAHRLMRYFAKPRPAQGEAFPELTDREREI
                     LELIARHETNPEIAGKLHLSPKTVRNHVSNIFTKLQVADRAQAIIRAREAGLGREKA"
     gene            complement(3623..5782)
                     /locus_tag="VHM69_17210"
     CDS             complement(3623..5782)
                     /locus_tag="VHM69_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2742181.1"
                     /translation="MSRPFTPELPGATPDEQSTAKPLRGRLLIVIRAAWMVVAALTVS
                     LFVAGVPVEFALLHEPCPTVRCTTGQLPPAGLHALEDLGLSRGSFAAYFVAMDAVFAT
                     VWVAVAALIFWRRSDDRMGLFASLALLTFGTATYTFTLAALTIRHPAWDIPVAFLHFL
                     GSASFGLFLYLFPDGRFVPRWARWVALAWIAWQLPPYFFPDWHLDPDAWYLWAQAIVW
                     PAGLGTALYAQAYRYRRVSSARQRQQVKLVVFGIASALSVFIALNLALGAFAPAPTSP
                     STLAALLIGYLLVYVALLLIPLSISVAMLRHHLFDVDFVINRTLVYGVLTASVVLLYV
                     LVVGGLGAALQVQGSLIVSLIATGLAAVMFQPLRERLQRGVNRLMYGERDDPYAVLSR
                     LGSRLESTLAPHAVLPAVVVTVKEALRLPHVAVEVAKNGAFETAASAGEPLGGYLRLP
                     LLYGGERVGRLVLGMRPGEEDFSPADRRLLDDLARQIGAAVHAARLTDEAVRLSEDLQ
                     RSRERLVTAREEERRRLRRDLHDGLGPQLAAVTMKAEAACDLLAVDPGRSEVLLEDII
                     ARTQEAITDVRRLVYGLRPPALDEIGLLGALRSQAAHGVHNGLRVAVEAPEDLPPLSA
                     AVEVAAYRIVLEAVNNAALHAGARSCAVSLAVDGDLLRLEITDDGRGIPDDRPAGVGL
                     HSMRERAEELGGSCVVEASPEGGTRVRAVLPLARGEA"
     gene            complement(6053..6895)
                     /locus_tag="VHM69_17215"
     CDS             complement(6053..6895)
                     /locus_tag="VHM69_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2742182.1"
                     /translation="MATTTATPSRTAATRTFPLIAFFVLAYALSWIILVPAGLGLLPD
                     SAGVLAWVAPFGPAVSAFVVTALTGGKLAVGRLLRRMVQWRVGVGWYLLVLFGVPLVE
                     LLGAFIVLGSVPLDDLAQNWPLIFSSYLPQVLVAVVVIGLAEETGWRGFALPRLQEWR
                     GPLVGTAVLAVVWAMWHLPNLLFGGYTGASYALWLAQCLAVAFIYTWVYNNTGGSILL
                     AALLHAAINMGSGLVAGLVPGLEDAFNVHLYGAIALAFTVVAVVLVVATRGRLGYRAE
                     GSSP"
     gene            complement(7140..7973)
                     /locus_tag="VHM69_17220"
     CDS             complement(7140..7973)
                     /locus_tag="VHM69_17220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2742183.1"
                     /translation="MNARGGSVESSHAREATRGEQYSLAKILGIWALAVVPMGILGWI
                     VFPLLAPDPGSDPLGFGLTRLVLLTLGLIWLFVLSMIIVRREEGDLRWTTIKRRLRLN
                     TPRDPSTGQPRARLWLWVVPFLVAVAAVELVLDSPLEHAWVSVFPFLAEPQGYSLDAI
                     FGSQEILQRLEGAWWFLALFVVQAAFNTVLGEEFLFRGVLLPKMEGVFGGSSWVANGV
                     LFGLYHVHQPWGIPNSVLTGLLYTFPAHRYRSTWMSIILHSAQSVYLAFLVLGVVLGL
                     A"
     gene            complement(8081..8791)
                     /locus_tag="VHM69_17225"
     CDS             complement(8081..8791)
                     /locus_tag="VHM69_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742184.1"
                     /translation="MGATNINRWIGIGLLGLPLYGVLTFFSSLDPQPDPSTHYEAWAR
                     FVTTDGYVLKHLLLSVLGIVFVIFGTFALGAYLTRSRAGRMGLWAMVIAIFGHALFLT
                     IGGVSTFSAPWEGQIVLSGMEEINELPSILANTLQTATFGVSILLGFVGNVLLGVAVW
                     RSGILPKWAGALWAAAPVLMYVFGLVYAMTIGAQATPPTVPAGAVLLAIGGAWMAWSV
                     LSRPSAATVGIQAQPRVQ"
     gene            complement(8978..10366)
                     /locus_tag="VHM69_17230"
     CDS             complement(8978..10366)
                     /locus_tag="VHM69_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2742185.1"
                     /translation="MKDAELPRTLETLAHSLRERHLSPVEVVRGLLERIEADESNSFI
                     TVTAERALDGASRAETEILAGRYRGPLHGVPVGLKDLIYTRGVRTTMGSGFFAEYVPD
                     HSATVARKLEEAGSVLIGKTNTHEFAYGPTGDRSYFGPARNPHDTRGITGGSSSGSGA
                     AVAAGLCYGALGSDTGGSIRIPAALCGTVGMKPTFGRVSKNGVFPLSWSLDHVGPLTR
                     TVSDNATMLNVLAGHDPADPHSADLPAEDFTRDLETDLRGTSLGLPSSFYFEHVDEEV
                     ESRVREAAEVFASLGAEVREVEIPNLWDTLKAQRLILAAEAYAVHEERLKNEPEKFGE
                     EVRERLIDGERPKAYRYANARRRGMLATDEFDRVLDGVDVILTPSVPITATEIGQREI
                     SIEGYEEDVRSALTRFTGPTDLTGHPSLSVPCGTTNSGLSVGLQLIGRLFDEATLYGF
                     GHAYEEAVGPKA"
     gene            complement(10586..>12084)
                     /locus_tag="VHM69_17235"
     CDS             complement(10586..>12084)
                     /locus_tag="VHM69_17235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2742186.1"
                     /translation="LIEGEIQTYTAEKRYIRKDGSAVWVNLTVSLVRGMSGEPGYFIS
                     VVEDISERKKTEEERDLLLVREQLARAEAVEARRRLALLAAAGPALSASLDYEETMEG
                     ITRLLVPELADWCLLDIVEEDGNVNQLAAAHADPEKENLVRRLKEHRRFGEGDPGSTA
                     EVLRTGKSVVLPDLPDATFHERALGRGEHLDIVLRLEPRSLMCVPLLARGKTLGAITL
                     VSSRPDRRYGEEDLLLAENLAYRCALAADNARLYRDRSEIARVLQRSLLPPHLPEIPG
                     VEVGAEYLPVGEANDVGGDFYDLINTVEDGWVCAIGDVRGKGVEAASVTALARYTIRA
                     VTLKDDRPSEILAALNEAMIRQLPEDRFCTAACVRLEPDEGSPGVGADVSRAGHPPPL
                     VVRADGTVEEVGCPGKVLGVFADAELTDTSLRLMAGETLVLYTDGVTEARSPDGDFFG
                     EDRLRHLLHSCSGCDAETIARRIKSVVLAFQEGYPRDDLAVLVLRVMD"
BASE COUNT         2220 a         3916 c         3954 g         1994 t
ORIGIN      
        1 gtcctggagc gccgcgatgt catcgggggc gctactgtca cggaggagcc gtggcctgga
       61 tacaggctct cgacgtgctc ctacgtatgc agcctgcttt tgcccgaggt gatccgggac
      121 ctcgacctga agcgccacgg ctacgacgtg cgcgccctcg acccgcagta cttcgtccca
      181 ttccccgacg gccgccacct tttctcgttc ctcgacggcg cgaagacgaa ggagcagatc
      241 cgcaagttct ccgagaagga cgcgcgctcc tacgacgcct actgggcgat gtggggccgc
      301 atcatcgccc gcatgcgtcc gctcctaacg agatccagcc ctactgtcga ggagatagaa
      361 cgcgccttcg aaggcccaca gggcgagcaa gactgggata cgttgacccg gtcgagcgtc
      421 gcggagttac tggacgagta cttcgaatcc gaggaggtca aggcgccgct gtgcgtgggg
      481 ggtgtgatcg ggacgaacgc agggccccgc gaccctggta ccgcatacgt gaagtaccac
      541 cacatcatcg gcaacctgga cggccaccag ggcgcctggg gcttcgtccg gggcggcatg
      601 ggcgccgtcg cagaggccat cgctctctcc gcgaaggcgc aaggggccga gatcctaact
      661 aaggccgagg tcacggagat agagatccag gacggcgcgg cgcgcggcgt ccacctcgca
      721 gacggacgct ctttcgaggc cgatgtgatc ctctcgaacg ccgacccgca caggaccttc
      781 ctcgggatgg tcggtgagaa gcacctgccc cctgaactgg tcgatggcgt tgagaatgcc
      841 aggacgaagg gttcggtagt aaaagtcctg ctggcgctcg gggagctgcc tgacttcaca
      901 gcgttgcctg ggaggaaggt cgggccgcag cacaccggcg cagttgtcat caacccgtcc
      961 atagactacc tccaaagggc gtgggaggac tgcgaacagg gagagccttc cgaaaagccg
     1021 ttcatggagg gctatataca gagcgccacg gagagcggtc tcgccccgac gggcaaacac
     1081 acgatgagcc tgttctgcca gtacgcgccg tacgaactgg acgaaggtac gtgggaacat
     1141 cgccgggccg ggatcggggc gaacatcata gagaccttcg ctgagtacgc gccgaatctg
     1201 cctgacgcaa tagagcacat cgaggtcctc ggtccgccgg acatagaaga gaagatcggg
     1261 atcaccggcg gcaacatctt ccacggtgag atcctgcctg acaagctgtt cgggggacgc
     1321 ccggtccccg gctactcggg ctaccgcaca ccgatcgaga acctctacct ctgcggcgca
     1381 ggcacatggc ctggtggggc ggtcttcggc gcgcctggcc gcaactgcgc gatggaggta
     1441 ttggccggtc agcacgagcg tctcgcactc agatagacaa agcacgcgga aggagatcca
     1501 gatgcaaact gagaccaagc gggtaaagaa cttcgtcggc ggggagcacg tagaccccgc
     1561 ggacggccgt ttctacgatc tcgtcaaccc gtccacgggc gagacgttcg cgcaggcccc
     1621 ggcctctggc aagggggatg tggacagggc gttcgaggcc gccgcgaagg cgttcgagtc
     1681 gtggcgcgat acaactcctt cagagaggca gttggcgctg ctcaggctcg ccgatgccat
     1741 cgaggaacgg ggcgaggagc tcgtccgcgc cgagtcggag aacaccggca aacccttcgg
     1801 gctcaccatg gaggaggaga taccgccgat ggcggaccag atccgctttt tcgccggcgc
     1861 ggcccgtatc ctggaaggga agtcagcggg cgagtacctc gaagggtaca cgtcgttcat
     1921 ccgccgcgag cccgtgggtg tcatcggcca ggtgacgccg tggaactacc cgatgatgat
     1981 ggctgtctgg aagttcgccc cagccatcgc cgcaggcaat accgtcgtct tgaagccttc
     2041 agacaccacc cccgtgacga ccgtgatgct ggccgagatc gccgcggagt tcctccccga
     2101 tggcgtcttc aacgtggtct gcggcgaccg tgacactgga cgcgccctcg tcgaacaccc
     2161 gatcccacag atggtctcca taacaggctc ggtccgggcc ggcatgcagg tggccgaggc
     2221 tgctgcgcgc gaactgaagc ggacccacct ggagctgggc ggcaaggccc ctgttatcgt
     2281 cttcgacgac gccgacctcg aagaggcggc ggaggccata gcgggcgcag gctacttcaa
     2341 cgctggccag gactgcaccg ccgcaacccg cgtgctcgcc gcacccggca tccacgacga
     2401 cttcgtggcg gcgatcaccg agcaggcgaa gaacaccagt acgggaagcc ccgacgagga
     2461 cgtggactac ggtccgttaa acaaccccac ccagctggag cacgtctcag gcatggtcga
     2521 gaggctaccc gaccacgccg aggtcaccac aggtggtgag aaggtcgatg ggtcgggcta
     2581 cttttactca cccaccgtcg tctcggggct gaggcaggac gacgaggctt cccagacgga
     2641 gatcttcggc cccgtcgtca ccgtgcagtc cttctcgaat gaggacgagg cggtcaggtg
     2701 ggccaacggc gtgaagtacg gcctcgcctc cagcgtgttc accagggacc acgccagggc
     2761 gatgcgggtc agccgacggc tggacttcgg gtgcgtctgg atcaacaccc acatccccct
     2821 cgtcgccgag atgccacacg gcggcttcaa gcactcgggg catggcaagg atctctccat
     2881 gtacggcctc gaagactaca cccgtatcaa gcacgtgatg acgagcctcg gatagtcgta
     2941 agctggacgt aggcgctccg gagcgtctac gccttctctc ttccgagccc ggcttcgcgg
     3001 gctcggatga tagcctgcgc gcggtcggcg acctgaagct tggtgaagat gttcgagacg
     3061 tggttcctga cggtcttggg gctgaggtgc agcttgccgg cgatctcggg gttcgtttcg
     3121 tgcctggcta tcagctccag gatctcgcgc tcgcggtcgg tgagctcggg gaaggcttcg
     3181 ccctgcgccg gacggggctt ggcgaagtac cgcatcaagc gatgtgctat cgccgggccg
     3241 aagatggcct caccgctgac gacggcccgg atggagcgca ggatctcggc cttgagcgcg
     3301 cctttgagga ggtagccgcg ggcgccggcc ttgagtgcgg cgaagacaga gtcgtcgtcc
     3361 tcgtacatgc ttatgacgag caccgagatg tgcgggctgg tgtggaggat gcggcgggtg
     3421 gcctctatgc cactgatgcc tggcatgttg aggtccatca ggatcacatc cggctgcaac
     3481 ttctctgcga gccggatggt cttctccccg tccccggcct ccccaacgac ctccacgtca
     3541 ggcgcggaga gaagcagggc gcgcatcccg tcccggaagt gcgggtggtc gtcggagatc
     3601 aggacccgta tcggctccac gctcacgcct ccccgcgcgc gagcggcaga acggcccgaa
     3661 cacgagtgcc gccctctggc gatgcttcga ccacgcagga accgccaagc tcctcggccc
     3721 gctcgcgcat cgagtggagg ccaacgcccg cgggcctgtc atcggggatg cctcgcccgt
     3781 cgtcagtgat ctccagccgc aagagatcgc cgtccacggc caacgacacg gcgcaagagc
     3841 gcgccccggc gtgcagggcc gcgttgttca cggcctcgag gacgatgcgg taggctgcga
     3901 cctctacagc ggcggacaga ggtgggaggt cctcaggggc ttcgacggca acgcggaggc
     3961 cgttgtgcac gccgtgcgca gcctgagacc ggagcgctcc gagcagaccg atctcgtcaa
     4021 gggcgggcgg cctcagcccg tagaccagac gtctcacgtc agtgatggcc tcctgcgtcc
     4081 gcgctatgat gtcttccagc aggacttcgg aacggccagg atccaccgcg aggaggtcgc
     4141 acgccgcctc ggccttcatg gtcacggcgg caagctgcgg cccgaggccg tcgtgcaggt
     4201 cacgacggag cctgcggcgc tcctcttcac gcgcggttac cagccgctca cgcgaacgtt
     4261 gcagatcttc cgagagccgc acggcctcgt ccgtgagcct ggcggcgtgg acggcggccc
     4321 cgatctgcct ggcgaggtcg tcgagcagac gccggtcagc aggcgagaag tcctcttcgc
     4381 cgggcctcat ccccagtacc agccttccca ccctctcgcc gccgtatagg agcggcagcc
     4441 ggagataccc tccgagcggc tcgcccgctg acgccgccgt ctcgaaagcg ccgttcttcg
     4501 ccacctcgac ggcgacatgg ggcagcctga gcgcctcctt caccgtcacg accacggcag
     4561 gcaggacggc atggggagcg agggtggatt cgaggcggga tcccagccgc gagaggacgg
     4621 cgtaggggtc gtcgcgctcg ccgtacatca ggcggttcac gcccctctgc aaccgctcgc
     4681 gcaggggttg gaacatgaca gccgcaagcc ccgtggcgat caacgagacg atcagactcc
     4741 cctgcacctg caacgccgca ccgagtccac ctactaccag cacgtagaga aggacgacgc
     4801 tggccgtcag gacgccgtag acgagggtgc gattgatgac gaagtcgacg tcgaagaggt
     4861 ggtggcgcag catggcgacg ctgatgctca aagggatgag gaggagggca acgtagacga
     4921 gcaggtaccc gatcaggagc gcggccagcg tgctcggcga cgtgggagcc ggggcgaagg
     4981 cgcccaacgc caggttcaga gcgatgaata ccgagagcgc cgacgcgata ccgaagacta
     5041 ccagcttgac ctgctgccgc tgcctcgcgc tggagacgcg tcggtaacga tacgcttggg
     5101 cgtagagcgc ggtgcccagg ccggccggcc acacgatggc ctgggcccag aggtaccagg
     5161 cgtccgggtc caggtgccag tcaggaaaga aatacggggg caactgccag gcgatccacg
     5221 ccagcgccac ccaccgcgcc catcggggca cgaaccgccc gtccgggaaa aggtacagga
     5281 acaggccgaa ggaggccgag ccgagaaaat gtaggaaggc gaccggtata tcccaggcag
     5341 gatggcggat cgtcagagcc gccagggtga aagtgtaggt cgccgtcccg aacgtgagca
     5401 gcgccaggga ggcgaacagc cccatgcggt cgtcggatct gcgccagaag atcagggcag
     5461 caaccgcgac ccacacggta gcgaagacag cgtccatcgc gacgaagtag gcggcgaagg
     5521 aaccccgcga gaggccgaga tcctccaggg cgtgcaggcc ggcgggggga agctgtcccg
     5581 tggtgcatcg gacggtcggg cacggctcgt gcagcagcgc gaactcgacc ggcacgcccg
     5641 cgacgaagag cgagacggtc agggcggcca ccaccatcca cgcagcgcga ataacgataa
     5701 gcaggcgccc gcgcaaaggt ttggcggtcg actgctcgtc tggcgtcgcc cccggtagtt
     5761 cgggagtaaa cggacggctc acgcgcacgc tcctcgcaaa ggtatctgag acgccctggt
     5821 cgaaattctc ttgcggtcca tttctgtgct atccgtcgcc ttcatgatgc cacgctcgcc
     5881 cgtgtttcgc ctcgacgcct ccgaaacgga tcgatctcag cttgtccata tcaagcttct
     5941 ccttcagtgt gtcaccgctg gcggtcttgt gccgagccca acgccaatta gcgcttcaac
     6001 gccgtgccat gcgcgagaac ccgcgcacga cgaatcagct tcggagtggg gcctacggcg
     6061 atgatccctc cgccctgtag ccgagcctac ccctggtggc gacgacgagc acgacggcta
     6121 cgacggtgaa cgcgagagcg atggcgccgt acagatgcac attgaaagcg tcctccaggc
     6181 cgggcaccag tcccgcgacc aagccggagc ccatattgat cgccgcgtgc agcagtgccg
     6241 caagcaggat gctaccgccg gtgttgttgt acacccaggt gtagatgaac gcgactgcca
     6301 ggcactgcgc cagccacagc gcgtaggagg cgcccgtgta gccgccgaac agcaggttcg
     6361 gcaggtgcca catagcccac accacagcca ggacggcggt gcccacgagc gggccacgcc
     6421 actcctgaag cctcgggagg gcgaagccgc gccagcccgt ctcctcggcc agcccgatca
     6481 ccacgaccgc gaccaggact tgaggcaggt agctactgaa gatgagcggc cagttctgcg
     6541 cgaggtcgtc gagaggcacc gagcctagca cgatgaacgc cccgagcaac tcaacgagcg
     6601 gcacgccgaa gagtaccagc agataccagc ccaccccaac ccgccactga accatccgcc
     6661 gaagcagtcg gcccacggcg agcttgccgc cggttagggc ggtcacgacg aaagctgata
     6721 cggccgggcc gaagggggcc acccaggcca gtacaccggc gctgtccggc agcaggccga
     6781 gacctgcggg caccaggata atccagctca gagcgtacgc gaggacgaag aaggcgatca
     6841 gcgggaaggt tctcgtcgcc gcagtgcggg atggtgttgc ggtggtagtc gccatttcct
     6901 tgtctccttt tagctcgttt ctcggatcgc cgtgcgttag atcctccaac tgaagcttcg
     6961 tccaggagga tcacttccgc cctcgatggt agcgtgcgct tttatcttca cttgtcctgc
     7021 ttccgacgcc tcgctcctgt cagggttgtt tgtgttgcga cttgtacaaa ttgatagctc
     7081 ccttcgtttt cgtctacgtg ctttgacagg accccgtggc gtccgccgcg gtctcgggac
     7141 taagccagcc ccaacacgac cccgagcacc aggaaggcga ggtacacact ctgtgcggag
     7201 tggaggatga tagacatcca cgtgctcctg tagcgatggg cgggaaaggt gtacaaaagt
     7261 ccggtgagaa cggagttcgg tatcccccag ggctggtgaa cgtgatagag accgaagagt
     7321 acgccgtttg ccacccagct gctgcctccg aacacacctt ccatcttggg caagagcacg
     7381 cctcgaaaga ggaactcctc tccaaggacg gtgttgaacg cggcctgaac gacgaacagg
     7441 gctaagaacc accaggcgcc ctccagccgc tgcaggattt cctgcgatcc gaagatcgca
     7501 tcgaggctgt agccttgggg ctccgcaaga aacgggaaga ccgacaccca agcgtgttcc
     7561 aagggggagt ccagcacgag ttccaccgcc gcgacagcaa cgaggaaagg caccacccac
     7621 aaccagagcc ttgcgcgggg ttggccagtt gacgggtccc gcggggtatt gagcctgagc
     7681 ctgcgcttga tcgtcgtcca gcgcaggtcg ccttcctctc gacggacgat gatcattgag
     7741 aggacgaaca gccagatcaa accaagcgtg agtagcacca gcctcgtgag gccgaacccg
     7801 agaggatctg acccaggatc aggtgccagc agcggaaaga cgatccaacc caggatgccc
     7861 atcgggacga ccgcaagtgc ccagataccc aggatcttcg caagggaata ctgttcaccg
     7921 cgcgtggctt cccgcgcatg actactctca accgaccctc ctctagcgtt catggaaacc
     7981 actccttact cgtttcactg gcgtatgcac cactgacaga ggatcctgtt ccattgctag
     8041 ccgttgtcgt gcagcgtggc cgtcgctcca gaggtgtcgg ttattgcacc ctcggttggg
     8101 cttggatgcc taccgtcgcg gcggagggtc ggctcaggac gctccatgcc atccacgctc
     8161 cgcctatcgc cagaagcacc gccccagcgg gcaccgtcgg aggggtggcc tgggcgccga
     8221 tcgtcatcgc gtacaccaga ccgaagacgt acatcaacac gggcgcggcc gcccagaggg
     8281 ctccggccca cttgggcagg atcccggagc gccagacggc cacgccgagg agcacgttgc
     8341 ccacgaaccc tagcaggatg gacactccga aggtcgccgt ttgcagggta ttcgcaagaa
     8401 tgctaggcaa ctcgttgatc tcctccatgc ccgacaggac gatctgcccc tcccagggag
     8461 cggagaaggt ggagaccccg cctatcgtca ggaacagggc gtgtccaaag atggcgatta
     8521 ccatagccca caaccccatt cgtcctgcgc ggcttctggt caagtaggct ccgagggcaa
     8581 acgtgccgaa gatcacgaag acgatgccga ggacgctgag gagcaggtgc ttgagcacgt
     8641 agccgtcggt ggtgacgaag cgagcccagg cctcgtagtg ggtactcggg tcgggttggg
     8701 gatcgaggct cgaaaagaag gtcagtactc cgtagagggg cagacccagc aggccgatgc
     8761 cgatccacct gtttatgttc gttgcgccca tcagtagctc ctttcaccgt cgtccctcta
     8821 ttaaggttat gggcagccta cggcgtgcgg tatcccgccg tcatgagaca gagtcccgat
     8881 caggccggga agaatgtccc gactattgtg atcaatgatc gtcgatcaaa tcgacgcgcc
     8941 ggtgcggacc gcagccggag atagtcacta gagcgtgtca ggccttgggg cctaccgcct
     9001 cttcgtaggc gtgcccgaac ccgtagaggg tcgcctcgtc aaagagccgt ccgatgagtt
     9061 gaagtcccac ggaaaggccg gaattcgtgg taccacaagg tacggagagg ctcgggtggc
     9121 cagtcaggtc ggtcggtccg gtgaagcggg tgagcgccga gcggacgtcc tcctcgtagc
     9181 cctcgatgct gatctcgcgt tgcccgatct ccgtcgccgt gatggggacg gacggggtga
     9241 ggatcacgtc cacgccgtcc agaacacggt cgaactcatc cgtggcgagc attcctcgtc
     9301 gtcgggcgtt ggcgtagcgg tacgccttgg ggcgctcacc gtctatcagg cgctcgcgga
     9361 cctcttcgcc gaacttttcg ggctcgttct tcaggcgttc ctcgtgcacg gcgtaggcct
     9421 cggcggcaag gatgagtctc tgcgctttga gggtatccca gaggttgggg atctcgacct
     9481 ctctcacctc agcgcccaag gaggcgaaga cctccgccgc ctccctgacg cgtgactcca
     9541 cctcctcatc gacgtgctcg aaatagaagc tcgaaggtaa gcctaaggag gtccccctga
     9601 gatcggtttc gaggtcccgc gtaaagtcct cggcaggcag gtctgcggag tgagggtctg
     9661 ctggatcatg gcccgcaagc acgttcagca tcgtggcgtt gtcgctgacg gtgcgggtga
     9721 gcggtccaac gtggtcgagg ctccatgaga gcggaaagac gccgttcttg ctcacgcgcc
     9781 cgaaggtcgg cttcataccg acggtgccgc agagggccgc agggatacgg atggagcccc
     9841 ctgtgtccga gccgagcgcg ccgtagcaca gtcccgcggc gaccgcggcc cccgagccgc
     9901 tgctggagcc gcccgtgatc ccgcgcgtgt cgtgagggtt gcgcgccggc ccgaagtacg
     9961 aacggtcgcc cgtcgggccg taggcgaact cgtgggtgtt ggtcttcccg ataaggaccg
    10021 aaccggcttc ttccagcttc cgtgcgaccg tcgcgctgtg gtccgggacg tactccgcga
    10081 agaatccgga acccatcgtg gttcggacgc ctctggtgta gatgaggtcc ttcaggccga
    10141 ccggtacgcc gtggagcggt cctctgtagc gtccggccag tatctccgtc tcggcccgcg
    10201 aggccccatc tagcgcccgc tccgccgtca cggttatgaa ggagttcgac tcgtccgctt
    10261 cgattcgctc cagaagcccc ctgacgacct cgaccggcga gaggtggcgc tcgcgcagtg
    10321 agtgggcgag cgtttcgagc gtgcgcggca attctgcatc cttcaagtct ttactcctct
    10381 gctgcgggag atccgcagta tctcatagcg ttcctgggtg tttccgatat catcggcgga
    10441 tgatcccacg cgccagaaag cgctgctgcg tttcgacggg agggtttggc ttctgctctt
    10501 cgccatgctc gccttccggt tcgggcacgg cctttgctta cagcttgcgg cagtggacct
    10561 cgttgcgctg gccgctctcc gccgctcagt ccatgacgcg taggacaagg actgccaggt
    10621 cgtcgcgcgg atagccctcc tggaacgcaa ggacgacgct tttgatcctc cttgcgatgg
    10681 tctccgcgtc gcagccggag caggagtgca ggagatggcg aagcctgtcc tcgccgaaga
    10741 agtcgccgtc gggggagcga gcctcggtga cgccgtctgt gtataggacg agcgtctcgc
    10801 ctgccatgag gcgcagcgag gtatccgtga gctccgcgtc ggcgaacacc ccgagcacct
    10861 tgccggggca gcctacctcc tcaaccgtac cgtccgcgcg cacgacgagc ggcggcggat
    10921 gtccggcgcg cgagacgtcc gccccgacac cgggagagcc ttcgtcgggc tccaggcgga
    10981 cgcaggccgc ggtgcagaac cggtcctcgg gtagctggcg gatcatcgcc tcgttcagcg
    11041 ccgcgaggat ctcggagggt ctgtcgtcct tgagggtgac ggcgcggatg gtgtagcggg
    11101 cgagggccgt caccgacgcc gcctcgactc ccttgccgcg cacgtccccg atggcgcata
    11161 cccacccgtc ctcgacggtg ttgatcaggt cgtagaaatc tcctccaacg tcgttggcct
    11221 ccccgacggg caggtactcg gcgccgacct cgacgcctgg tatctcgggc aggtgcggcg
    11281 gaagaaggct cctctggagg acgcgtgcga tctcactgcg gtccctgtag aggcgggcgt
    11341 tgtccgcggc cagggcgcag cggtaggcca ggttctctgc cagcaggagg tcctcttcgc
    11401 cgtagcggcg gtccggcctc gatgagacta gggtgatggc gccgagggtc ttcccccgcg
    11461 ccagtagcgg cacgcacatc agggagcggg gctccaggcg gagtacgata tcgaggtgct
    11521 ctccgcggcc cagcgcgcgc tcgtggaagg tggcgtcggg gagatccggg agcaccacgg
    11581 acttccccgt ccgcagtacc tcggccgtgc tccctgggtc cccctccccg aaccgcctgt
    11641 gctccttgag gcggcgcacg aggttctcct tctccgggtc ggcgtgcgcg gcggcgagct
    11701 ggttcacgtt tccgtcctcc tcgacgatgt cgagcaggca ccagtcagcg agctcgggca
    11761 cgaggaggcg tgtgatcccc tccatcgtct cttcgtaatc gagcgaggcc gagagcgccg
    11821 gtcccgcggc cgcgagcagg gccagtcgcc gcctcgcctc caccgcctcg gcccgcgcga
    11881 gttgctccct cacgagcaac aggtcacgct cttcctcggt cttcttccgc tcactgatgt
    11941 cctcgaccac cgatatgaag tacccgggct cccctgacat gccgcgcacg agagatacgg
    12001 tcaggttcac ccagaccgcg gagccgtcct tcctgatgta gcgcttctcg gctgtgtaag
    12061 tctggatctc accctcgatc agcc
//
LOCUS       DATAIR010000725         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_71263, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000725 DATAIR010000000
VERSION     DATAIR010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71263"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VHM69_17240"
     CDS             <1..299
                     /locus_tag="VHM69_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742187.1"
                     /translation="PKASRAAAEEDSPSVSETSPTAEEMRGVRCPVALVRASQGFFPG
                     TDPLFSDEARDAVAGSLDIREEIVLEGANHYTMLWPEYTQIWAPRVFDSPFWER"
     gene            complement(631..1380)
                     /locus_tag="VHM69_17245"
     CDS             complement(631..1380)
                     /locus_tag="VHM69_17245"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742188.1"
                     /translation="MFDSLQQGLSDFFAFIPQLIGAIVILIVGYIVAKILQAVVARVL
                     KAVGFDGWMERSGIKQFFDRAQTNQTPATILGKLVFWFVFIIGITMATDALGIEQVSA
                     VFSQLIAYIPNIIAAVLILVLAGLLAQFVAGLVRGATGINILGTIAQAAIMVYAVFAA
                     LTQLQIAVQLTAPTFLIVLGAVALAAAIAFGWGARNVAQDIVENAYNRRDEARQKIHE
                     QGDSSASTGATGDGALEDEDRLAVRPLRRQE"
     gene            complement(1478..2113)
                     /locus_tag="VHM69_17250"
     CDS             complement(1478..2113)
                     /locus_tag="VHM69_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742189.1"
                     /translation="MTDRERGNTGAPRSDVEEGSTRNDSAPRRAGDVREEREADRRHR
                     ESELGTSWASVIFGWLAALGAGLILSGIVGGIVGAILGALGAKGGTEGGIAALVGLLL
                     TLLLAFLIGGYVAGRLASRSGLKHGLLVPLLALVVTLLLAIIGGAIGYSFIDQLSGVT
                     LPQGAQQQVPQQNLGTILTIPGILALLFPFIGGALGGIWGANTGRDRPYRP"
     gene            complement(2258..>3125)
                     /locus_tag="VHM69_17255"
     CDS             complement(2258..>3125)
                     /locus_tag="VHM69_17255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HEX2742190.1"
                     /translation="MDHEARSDRFTEVEDRYAGYTVYDQDYEKIGKVDDLFVDENDSP
                     EYLGVKMGFLGTRSTLIPFQMARVNDERQVIEVSADKETVKNGPTFDDDKEITPDFEN
                     EVYSYFGLEHTNGTDERGAYSDYYGEETGSTGPGMAMGDTETGEFREHDRESEGLSQP
                     GSDLEDEDELRVQRTEEELAAGTREREAGSMKVRKRVRTDREHIEVPTRHEEVSVERV
                     PVEGEASEAEIGDDEVRVPVTEEEVVVEKRPVAKEEVRIRKDVVENTETVEEDVRREE
                     IDVEDETERRNI"
BASE COUNT          511 a         1019 c          946 g          649 t
ORIGIN      
        1 agcccaaagc ctcccgcgcg gctgcggaag aggacagccc ctccgtctcg gagaccagcc
       61 cgaccgcgga agagatgcgc ggcgtgcgat gtcccgtcgc gctggtcagg gcttcgcagg
      121 gcttcttccc tggcaccgac ccgctcttct ccgatgaggc tcgcgacgcg gtggccgggt
      181 cactcgacat ccgcgaggag atcgtgcttg agggcgcgaa ccattacacg atgttgtggc
      241 ccgagtacac acagatttgg gctcctcggg tattcgattc accattctgg gagcgctgag
      301 ccagaacgtg cgtcggactt cgcctcatac aaacggtgtc ccctgcctga gaaagtagga
      361 ccgaaacctt actgtaccgg gtcccggctc gggagcagat cagtgatctg gactaacgac
      421 ttgccatcat ccgacgatga ggcagagatt gctactctct tcaagggcac cgtcacacag
      481 tgttgacgtc cgcgtcttcg ccctatgcga cgatgagatt ggtgcgaaga ggcggatact
      541 ttcgtaagtt acgccttccg ggaacgcacg tagcgcaaaa aggcggaact cctcggagtc
      601 ccgccttcga acgccccggt tttcggggtt ctactcctgc cggcgcaggg gcctgacggc
      661 gagtctgtcc tcgtcttcga gcgcgccatc gccggtcgcg ccggtggacg cggaggagtc
      721 gccctgttcg tgtatcttct gtctagcttc gtccctgcgg ttgtaggcat tctcgactat
      781 gtcctgggcc acgttgcggg cgccccagcc gaacgctatc gctgccgcca gggccacggc
      841 accgagcacg atcaggaacg taggcgccgt aagctggacc gcgatctgaa gctgcgtcag
      901 agcggcgaag actgcgtaca ccatgatggc ggcctgggcg atggtcccga ggatgttgat
      961 gccggtcgcc ccgcgtacca gccctgcgac gaactgggcc aggagtcccg caagtaccag
     1021 gatcaggacg gcagctatga tgttcgggat gtaggcgatg agctgagaaa agaccgccga
     1081 tacctgctcg atgccaaggg cgtcggtggc catcgtgatg ccgatgatga agacgaacca
     1141 gaaaaccagc ttgccgagga tggtcgcagg tgtctggttc gtctgggctc tatcgaagaa
     1201 ctgctttatg ccgctacgtt ccatccaccc gtcgaagcct actgccttga ggactcttgc
     1261 cacgacggcc tgcaggatct tggccacgat gtagccgacg atcaggatga ctatcgcgcc
     1321 gattagctgc gggatgaagg cgaagaaatc gcttagtccc tgctgtagtg agtcgaacat
     1381 ctctatctct ctctttcttt agcttgcggt ttctacgttc tgcatgtcgc cggccaaggg
     1441 ggtctaccag cgtcggccgg cgacgtgatt ctctctttta cggccggtac gggcggtcgc
     1501 ggccggtgtt ggcgccccat ataccgccga gtgccccgcc gatgaacggg aagagcagag
     1561 cgagtatgcc ggggatggtc aggatagtgc caaggttttg ctgcggaacc tgctgctgcg
     1621 ctccctgtgg cagcgtcacg ccggagagct ggtctatgaa actgtagccg atcgcacccc
     1681 ctatgatggc cagaaggagc gtgaccacga gggccagaag cggcaccagg agaccgtgct
     1741 tgagaccgga gcggctggcg aggcgtccgg cgacgtagcc cccgatcagg aaggccagga
     1801 gcagcgtgag cagcagtcct acgagggccg cgatgccgcc ctccgtgcca cccttggcgc
     1861 ccagggcgcc gaggatagcg ccgacgatcc cgccgacgat gccgctcagg atcaggccgg
     1921 cgccgagcgc ggccagccag ccgaagatca cgctggccca actcgtgcct agctcgctct
     1981 ccctgtggcg acgatccgct tcccgctcct cgcgcacgtc tccggctctg cgcggggcgg
     2041 agtcgttccg ggtgcttcct tcctccacgt cggacctcgg cgcgccggta ttccctcgct
     2101 ctctgtcggt catactgtca ctcctttctg atctttgagc attcgcttcg acccactcgt
     2161 gaggatccca cttccctaca cgaaaggacg ggcccggtct tccgaccgga acccgccctt
     2221 ccacaggtcg ggtctcaaat gagaccgtgg gaacttctta gatgttacgc cgctcggtct
     2281 cgtcctcgac gtcgatctcc tcgcgcctga cgtcctcctc gaccgtctcg gtgttctcga
     2341 cgacatcctt gcggatgcgg acttcctctt tggccacggg ccgcttctcc acgacgacct
     2401 cttcctcggt aaccgggacc ctcacctcgt cgtcgccgat ctcggcctcg gaagcctcgc
     2461 cctctacggg tacccgttcc acacttactt cctcgtgcct tgttggcacc tctatgtgct
     2521 cgcggtcagt gcggacgcgc tttctgacct tcatggagcc tgcctcgcgc tcgcgggtgc
     2581 ctgcggcgag ttcctcctcg gtgcgctgca ccctcagctc gtcctcatcc tcgaggtcac
     2641 tcccgggctg gctcaggccc tcgctctcgc ggtcgtgctc gcggaactca ccagtctcgg
     2701 tatcgcccat cgccataccg ggaccggtcg agccggtctc ttcaccgtag tagtcgctgt
     2761 aagcgccgcg ttcgtccgtg ccgttagtgt gttcgaggcc gaagtaggag tagacctcgt
     2821 tctcgaagtc gggggtgatc tccttgtcgt cgtcgaaggt ggggccgttc ttgaccgtct
     2881 ccttatccgc cgagacttcg atgacctgcc gctcgtcgtt gaccctcgcc atctggaaag
     2941 ggatcagggt ggagcgggtg ccgaggaagc ccatcttcac acccaggtac tcggggctgt
     3001 cgttctcgtc cacgaacagg tcgtcgacct tgccgatctt ctcgtagtcc tggtcgtaga
     3061 ccgtgtaccc tgcgtaccgg tcctctactt ccgtgaacct gtcactgcgc gcttcgtgat
     3121 ccatc
//
LOCUS       DATAIR010000726         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_353004, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000726 DATAIR010000000
VERSION     DATAIR010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353004"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHM69_17260"
     CDS             <1..259
                     /locus_tag="VHM69_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742191.1"
                     /translation="TQLWKRYVSPEEVLRDWYEYSYRPIAEMIREERILDAFPDRTEL
                     DLYLWIVYHRERLALETRDEKISPQAAKDDILKNVPRRRRP"
     gene            293..1000
                     /locus_tag="VHM69_17265"
     CDS             293..1000
                     /locus_tag="VHM69_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX2742192.1"
                     /translation="MGVLAVVWAPVRILRDAAVHKRVLAAFIITALYAALGLAGSVVA
                     VLGGVTQVDGQPPGLPPGAMEKIRVATEVGLVVSSLLTPFVVWISVSLFMQFVTRYFG
                     GGARVVIGAPETNAAPDGSLAATMAAVGVSGVPLVISSAIGLLLGGLRVALGPQSTAG
                     IALGYVAGLLGLAAIVWYVVLVVIGVAQARNLGYAESAGSCAVSCVGCAGLIILVVIV
                     VGLGAALLIGPTISPQG"
     gene            1020..2045
                     /gene="sppA"
                     /locus_tag="VHM69_17270"
     CDS             1020..2045
                     /gene="sppA"
                     /locus_tag="VHM69_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX2742193.1"
                     /translation="METFSNGPSGQPERHEPRRRRRWPFVVGGLVAFGLVGLAIAAIV
                     LILAAGSPSAPEIYQEEYVAGDGSDKIAVVPVEGEIASADESLGGVQPTVTPEGLTDA
                     LRQAAGDTTVAAVVLEINSPGGGVTASDEMHQSILDFKESSGKPVVVSMEDTAASGGY
                     YISTAADRIVANETTLTGSLGVIFQLNNFAKAADKYGIKQVVIKSGKYKDIGNAFREM
                     KPEEREIFQSLVDDSYSEFVDVISEGRGIPKERVREIADGRVYSGSQAKELGLVDSFG
                     GLDEAAAIAGRLAGTSDTTVVRYVQEPTLVDTLLARLAPQEPQAVQIMDAAGLNLEPK
                     PYYLYRP"
     gene            complement(2208..3344)
                     /locus_tag="VHM69_17275"
     CDS             complement(2208..3344)
                     /locus_tag="VHM69_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX2742194.1"
                     /translation="MRRLILVCSAVFVAAGFVAITTLSAKATPVEQYLATDGTEAQNT
                     AGDGSQPETADVVSQGSALEADSSAVMAARADVAEEERLPEYSQVVDNNTDRGRFSAP
                     KWEEQSGERVNKESQVSYGGDYVYTGPGVGPARFTVKIPTSNDYTVYAWWPSFASNTD
                     AARFGIDTASGRQWTAVDQTRDGGIWIKLGTYAMEKGERTIQVTSNSAGTANVVADAV
                     AVVRGDVTMPPAEPTSGGTTSRSGEPIYSTSSRRDPTGRDVVRVAKRWLGTRYRWGTC
                     TRRRMSCTCETKKTYRHFGHRLPMSEIKQWRYDRSRRVSKSRLRIGDEVFFKENGRRG
                     GITHVGIYSGNGNIVHASSYFGKVTESKMRYVSGYFGARRYRLH"
     gene            complement(3574..>4669)
                     /gene="mmsA"
                     /locus_tag="VHM69_17280"
     CDS             complement(3574..>4669)
                     /gene="mmsA"
                     /locus_tag="VHM69_17280"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2742195.1"
                     /translation="MGETVRDVAKGIDNSSWRYPLGVVAAITPFNFPCMVPLWTLPVA
                     IGAGNTYILKPSERTPLCSVRIGELLAEAGLPDGVFNIVNGAREAVDAILEHPDIQAV
                     SFVGSQPVAEHVYTHGSAHGKRVQALAGAKNSMIVMPDAVLEKAVPNIISSAYGNAGE
                     RCLAGSVLVAVGEVADDLVGKLKEAASSMKVGPGKEEGSELTPVIRDSHRDKVRSYVD
                     LGEEEGAELVLDGREAPREEGFFMGPTLLDRVTGEMRVGREEIFGPVLSVVRVGSLDE
                     AIEFTNGSPFGNACSIYTEDGAAVRYWRENVEAGMLGVNVGVAAPMAFFPFNGVKNSF
                     YGDLHATGKDGVRFFTENKVEITRWLSDPA"
BASE COUNT          840 a         1501 c         1442 g          886 t
ORIGIN      
        1 cacccagctc tggaagcgat acgtctcacc ggaagaggtc cttagggact ggtacgaata
       61 cagctaccgc ccgatagcgg agatgatcag agaagagcgc atcctcgacg ccttccccga
      121 ccgcaccgaa ctggacctgt acctctggat cgtctaccac cgcgagcgcc tggccctgga
      181 gacgcgcgac gagaagatct caccacaggc cgcaaaggac gatattttga agaacgttcc
      241 ccgccgacgc cgaccctgaa tcaggcatgg cttatgtgat gggaggtagc gtttgggggt
      301 tctagcagtc gtctgggcgc ctgtacgcat cctgagggac gcggccgtgc acaagagggt
      361 actcgcggct tttatcatca cggccctcta tgctgccctc ggcctcgcgg gctccgtcgt
      421 ggccgtactc gggggcgtca cgcaggtcga tgggcaacct cctggcctgc cgcctggggc
      481 gatggagaaa atcagggtgg ccaccgaggt tggcctcgtc gtctcctccc ttctgacacc
      541 gttcgtcgtc tggatctccg tctcgctctt catgcagttc gtaacgcgct acttcggcgg
      601 cggggcgagg gtcgtgatcg gcgccccgga gactaacgcg gcccctgacg gatccctggc
      661 ggcgacgatg gcggcggtcg gcgtctcggg tgttccgctc gtgatctcct ccgcgatcgg
      721 actccttctc ggagggctac gggtggcgct cgggccgcag agcaccgcag ggatcgcact
      781 cggatacgtc gcggggctgc tcgggctcgc agccatcgtg tggtacgtag tactcgtcgt
      841 gataggggtg gcccaggccc gaaacctcgg ctatgctgag tccgcaggct cgtgcgccgt
      901 ctcgtgcgtc ggctgcgccg gccttataat tctcgtggtg atcgtggtcg gtctcggagc
      961 cgccctgctc atcggtccca cgatctcacc gcaagggtaa gtacggcgaa gggagcagta
     1021 tggagacttt ttctaacggg ccgtccggtc agcccgagag gcatgaaccc aggagacggc
     1081 gcaggtggcc cttcgtcgta gggggtctcg tggcgtttgg tctggtcggc ctggccatcg
     1141 cggcgatcgt cctgatcctg gccgcgggca gcccgtcagc ccccgaaatc taccaagagg
     1201 agtacgtcgc aggagacggg tcagacaaga tagccgtcgt gcccgtggaa ggtgagatcg
     1261 cctccgccga cgaatccctg ggcggggtgc agcccacggt aacacctgaa ggactcacag
     1321 acgccctgcg ccaggccgcc ggagacacga cggtcgccgc ggtagtgctc gagatcaact
     1381 ctcccggagg cggggtaacg gcgagcgacg agatgcacca gagcatcctc gacttcaagg
     1441 aaagcagcgg caagccggta gttgtctcca tggaagatac ggcggcctcg ggcggctact
     1501 atatctcgac cgctgccgac cgcatcgtcg ccaacgagac caccctgacg ggctccctcg
     1561 gcgtcatatt ccagctcaac aacttcgcca aggccgcaga caaatacggc ataaaacagg
     1621 tcgtcataaa gagcggaaag tacaaggaca taggcaacgc cttccgcgag atgaagccgg
     1681 aggagcgtga gatcttccag tcgctcgttg acgattcgta ctcggagttc gtggacgtga
     1741 tctccgaggg ccgcggcatc ccgaaagaga gggtgcgaga gatcgccgac ggccgcgtct
     1801 actcaggcag ccaggcgaaa gagctcgggc tcgttgactc cttcggaggt ctggacgagg
     1861 ccgcggccat cgcaggcagg ctcgcgggca caagcgacac gaccgttgtc cgctacgttc
     1921 aagagccgac tcttgtcgac accctgttgg cgcgcctcgc cccacaggaa ccgcaggcag
     1981 tgcagatcat ggacgcagca ggcctgaacc tggaaccgaa accctactac ctctacagac
     2041 catgaaaata ggaggccccg ttacagtccc ctctcaatgc gagacacgaa ctagaatgcc
     2101 gagagcgagt ccgcaggtcg aagcgcgctg aaaggtgcgt agcgccatgc tgaccagcgc
     2161 gaacagcagg aataagtcta ctgctgttcg cttcagacta ccgtgtctta gtgcagcctg
     2221 taccgcctgg cgccgaagta accggacacg tacctcatct tgctttctgt gaccttgccg
     2281 aagtagctgg aggcatggac gatgttcccg ttaccggagt agatgccgac gtgggttatg
     2341 ccgccccttc gcccgttctc tttgaagaag acttcatcgc cgatccgaag acgcgacttc
     2401 gagaccctcc tggaacgatc gtatctccac tgcttgatct cactcatggg cagcctgtgc
     2461 ccgaagtggc ggtaggtctt cttggtctcg cacgtgcagc tcatccgcct tctcgtgcac
     2521 gtaccccacc tgtaccttgt gcccaaccac ctctttgcaa ccctcaccac gtcgcggccc
     2581 gtgggatcac gtcgcgagct ggtcgagtat ataggctccc cacttctcga ggtagtccct
     2641 cctgaggtgg gttcagcggg aggcatggta acgtcgccgc gaacgacggc cacagcgtcg
     2701 gccactacgt tggcggtgcc cgccgaattc gacgtgacct ggatggtgcg ctcgcccttc
     2761 tccatcgcgt aggtacccag tttgatccag atgccgccgt ccctggtctg gtcgaccgcg
     2821 gtccactgcc tgcccgaagc ggtgtcgatg ccgaagcggg ccgcgtcagt gttgctggcg
     2881 aatgacggcc accaggcgta gacggtatag tcgttgctcg tggggatctt gaccgtgaag
     2941 cgcgcgggtc caacgcccgg gccggtgtag acgtagtccc cgccgtagct tacctggctc
     3001 tctttattca cccgctcgcc gctctgctcc tcccacttcg gcgcgctgaa acgccccctg
     3061 tcagtgttgt tgtcgacgac ctgcgagtat tccgggagcc gctcttcctc agccacgtca
     3121 gcccgtgccg ccatcacagc cgacgagtct gcttcgaggg ccgagccctg agagacgacg
     3181 tccgcagtct caggctggct gccgtcccct gcggtgttct gcgcctctgt gccgtcagta
     3241 gccaggtact gctcgaccgg cgtggccttg gcagagagcg ttgtgatcgc tacaaatccc
     3301 gcggccacaa agaccgccga acagaccaga ataagccgac gcatgtggcc ccccctcccg
     3361 agttcgtact tgcgtcggcg acattgtacc tgcgtcgtcc tcagaaagca ctagtttggt
     3421 aaagaatttt ctagattctt taaactagcg ttacatccgg gcaagcccga cgagtagacg
     3481 cggcgcaggg ctggacgcgc gtggcgtcca gccctgtctg agagcatccg ttcagaagcc
     3541 tgactccgcg gggtatttga aggtcgggta gtctcaggct ggatctgaga gccagcgagt
     3601 gatctcgacc ttgttctcgg tgaagaagcg gacgccgtcc ttgcctgtcg cgtgcagatc
     3661 tccgtagaaa gagttcttca cgccgttgaa tgggaagaac gccatcgggg ctgccacacc
     3721 gacgttcacg ccgagcatcc cggcctctac gttctccctc cagtacctga ctgcggctcc
     3781 gtcttctgtg tagatcgagc aggcgttccc gaaaggcgag ccgttggtga actcgatggc
     3841 ctcatccagc gaccccacgc gcaccacgga gaggacggga ccgaagattt cctcgcgacc
     3901 gaccctcatc tctccggtga cccggtcgag gagcgttggg cccatgaaga agccctcttc
     3961 ccttggcgcc tccctgccgt cgaggacgag ctcggctcct tcttcctcgc cgaggtcgac
     4021 gtaggagcgg accttgtcgc ggtgggagtc gcggatgacg ggggtgagct cgctgccttc
     4081 ttccttgcct ggaccgacct tcatcgaaga ggcggcctcc ttgagcttcc cgaccaggtc
     4141 gtctgcgacc tctcccacgg ccacgagcac gctcccggcg aggcatctct cgcccgcgtt
     4201 gccgtatgcc gaggagatga tgttcgggac ggccttctcg agcacggcgt cgggcatgac
     4261 gatcatcgag ttcttggcgc cggcgagcgc ctgcacccgc ttgccgtggg ctgagccatg
     4321 cgtgtagacg tgctcggcaa cgggctggga ccccacgaag gagacggcct gaatgtcagg
     4381 gtgctccagg atcgcatcga cggcctccct cgccccgttc actatattga agacgccgtc
     4441 gggcagcccc gcctcagcca gtagctcgcc gatcctcacc gaacagagcg gcgtcctctc
     4501 ggagggcttg aggatgtagg tgttacctgc ccctatcgca acggggagcg tccacagggg
     4561 gaccatgcag gggaagttga agggagttat ggccgccacc acccccagag ggtaacgcca
     4621 ggacgagttg tctataccct tcgccacgtc gcgcaccgtc tcgcccatc
//
LOCUS       DATAIR010000727         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_353316, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000727 DATAIR010000000
VERSION     DATAIR010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353316"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..807
                     /locus_tag="VHM69_17285"
     CDS             163..807
                     /locus_tag="VHM69_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742196.1"
                     /translation="MDKVRLLVADDHPMLREGLVAVLGTQPDFEVVGEAADGDEVVRL
                     AGDLDPDVILLDLEMPGTDGVEALERLRDAGSRARTVVFTAYDTDERILGSLRAGARG
                     YLLKGASRQEIFGAIRTVQAGGSLLVPSVTDRLLERIEGEENVPPNRRLTPRELEVLA
                     LLARGLKNSEIAARLFISERTVKFHVGSILAKLEAQNRTEAARIAVRRGLVETS"
     gene            999..1709
                     /locus_tag="VHM69_17290"
     CDS             999..1709
                     /locus_tag="VHM69_17290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2742197.1"
                     /translation="MALITGGSRGLGLALARSLAREKWNLIIDARGEDALESVRAELS
                     EHTRIIAIAGDVTDPEHRAALAEAAHEAGGVDALVNNASILGPSPQPELLDYPLDVLE
                     EVYRANVISPLAIVQAVREDLKPGARIINVTSDAAVEPYEGWGGYGSSKAALEQLTNI
                     LAAENPELRVYRVDPGDMRTRMQQEAFPDEDISDRPLPEESVPGFLELLTGDLPSGRY
                     EARALIARAGTSTLDQDS"
     gene            1719..2156
                     /locus_tag="VHM69_17295"
     CDS             1719..2156
                     /locus_tag="VHM69_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2742198.1"
                     /translation="MFSTELAEIVLIVEDVERSARFYREVVGLTPENEPENDRAWFWA
                     GEPGRTQRIALHKGPLLFEERSPLPDGERWGNVHYAFNVPHKRLEAAVDHTRQYDTEV
                     YGPVYFDWMEALSYYFYDPGGNLLEFWSADPTQAPGRKTNDES"
BASE COUNT          456 a          706 c          711 g          357 t
ORIGIN      
        1 tcatggacga tggacgaggc ttcgacccgt cgagcatcgc ggacgacagg cacggcatcg
       61 taggaatgag ggaaaggata gagatgctcg gcggctccct ccaggtggag agcagcgagg
      121 gcacgcgtgt cgaggttaca gtgccgctag aagggctgca gggtggataa ggtcaggctc
      181 ctcgtcgcgg acgaccaccc gatgctgcgc gagggcctcg tcgccgtgct cggcacccaa
      241 ccggacttcg aggtggtagg cgaagcggct gacggagacg aggtcgtcag gctggccggg
      301 gacctggatc ccgatgtgat cctgctcgac ctggagatgc cggggacgga tggtgtcgag
      361 gccctcgaaa ggctccgcga cgctggctct cgcgcgcgta ccgtcgtctt tacggcatac
      421 gacacggacg aaaggatact cggctctcta cgcgcgggcg cgcgcggata cctcctaaag
      481 ggggcgtcgc ggcaggagat cttcggcgct atccgaaccg tccaggccgg tggctccctc
      541 ctcgtaccct ccgtcacgga taggctgctc gagcgcatag agggcgaaga aaacgtccct
      601 ccaaaccgcc gcctcacacc ccgagaactc gaggtgctcg cgctcctcgc ccgcggcctg
      661 aagaactccg agatcgcggc ccgcctcttt atctcagaga ggacggtcaa gttccatgtc
      721 gggtccatcc tcgccaagct cgaagcccag aaccgtaccg aggcggccag gatcgccgtc
      781 cggcgcgggc tcgtggagac ctcctaaccc gcgaaacgtc tctgaatcta tccaccgccg
      841 agcttatttg tcaggacgga tgcgccgaat ccctccctgt accaaagtac aggccgaaaa
      901 ccgtccctca tgacgtttcc cggcttcggg tcaagcgata cattgtattc aaacaagttc
      961 aggaggaatg cgatatgacc ggaggatcac cacagcgcat ggcgctgatc actggcggct
     1021 cgcgcggcct cggcctggcg cttgcgcgct ctctggcccg ggagaagtgg aacttgatca
     1081 tcgacgcccg tggcgaggac gccctggaga gtgtccgcgc cgagctatct gaacacacgc
     1141 gcatcatcgc catcgcaggt gacgtaaccg accctgagca cagagcggcg ctcgcggaag
     1201 cggcacacga ggcgggcggc gttgacgcgc tcgtcaacaa cgcgagcatc ctcggcccca
     1261 gccctcagcc tgagttgctc gattacccgc tcgacgtgct cgaagaggtc taccgggcga
     1321 acgtgatctc accgctcgca atcgtccagg ccgtcaggga agacctgaag ccaggagcgc
     1381 gcatcatcaa cgtcacgagc gacgccgccg tcgaaccgta cgaaggctgg ggcggatacg
     1441 ggtcgagcaa ggccgccctg gaacagctca ccaacatcct ggcagccgag aaccccgaac
     1501 tgcgcgtcta ccgcgtcgat cccggcgaca tgcgtaccag gatgcaacag gaagccttcc
     1561 ccgacgaaga catcagcgac cgtccgctcc ctgaagagag cgtgccagga ttcctggagc
     1621 tactgaccgg cgacctgccg agcggacgct acgaggcccg cgcactcatc gcgagggccg
     1681 gtacgtctac cctggatcag gattcgtagg gagccgggat gttcagcacg gagctggccg
     1741 agatcgtact gatagtggaa gacgtggaga ggtcggcgcg cttctaccgc gaagtcgtcg
     1801 gacttacgcc cgagaacgaa ccggagaacg accgggcctg gttctgggcg ggtgagccag
     1861 gcaggacgca gcgcatcgcc ctccacaagg gtcccctgct tttcgaggag aggtccccgc
     1921 ttcccgatgg cgagcgctgg ggaaacgtac attacgcttt caacgtcccc cacaaaaggt
     1981 tggaagcagc cgtagatcac acccgccagt acgacacgga ggtctacgga cccgtctatt
     2041 tcgactggat ggaggccctc tcgtactatt tctacgatcc cggcgggaac ctgctggagt
     2101 tctggtccgc cgacccaacc caggcacccg ggaggaaaac gaacgatgaa agctaaggga
     2161 ctggtatggg tcggcacacg aacccgtaag ttcgacgaca cggttagatt cgttggggag
     2221 attctgggtc
//
LOCUS       DATAIR010000728         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_164364, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000728 DATAIR010000000
VERSION     DATAIR010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164364"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VHM69_17300"
     CDS             complement(<1..1128)
                     /locus_tag="VHM69_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2742199.1"
                     /translation="MRAEDIDGLTVGGLLDLVAGRRPDEDALVYADRDLRYTYREFEE
                     VVERCARALMALGLEKGDHVAVWGQNVPEWVILQFATGKMGAVLVTVNPAYKAHELRY
                     VLDQSDAAAVFLTAGVKGADFVDILGQAVPALAESAEGELSVEDLPHLRHVVLMGEGS
                     REGLPIMGFDEFMQRGKEVSQEELEERQASLGADEVINMQYTSGTTGFPKGVQLTHTN
                     IVKNAFHIGECMKLGPEDRVCIPVPFFHCFGCVLGTLNTVTHEGTMVPVESFDAEKVL
                     QAVDKERCTAVLGVPTMFIAELEHPDFEEYDTSSLRTGIMAGSPCPMEVMKQVVEVMG
                     ASEITIAYGQTESSPVITQTRTDDPLELRVSSVGRKLPEVEV"
     gene            complement(1220..2161)
                     /locus_tag="VHM69_17305"
     CDS             complement(1220..2161)
                     /locus_tag="VHM69_17305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HEX2742200.1"
                     /translation="MSPDFEQERAALIVNTRSRSGERTFFKALDRLQELDVSLGVTYA
                     IRDPARLPETVRAIVEDRPRYRVLILGGGDGSVSSVVDFLANRDVTLGLLPLGTANDF
                     ARTLEIPLDLGQACQTIANGRVVDVDLGLVHDNYFVNVASTGLSVRVTQALTPRLKRI
                     GALAYPTAAIRAFFQHEPFSARLTFPDGDLETVEYGRLLHLAVGNGRFYGGGMVLAPE
                     AGIDDRRLDVLAIELGRPRTLLGVVRYTRSGDFIPMEGVHHHRTERVLLETEPELPIN
                     IDGEVVTSTPQDFSVDHNALKVIVPPGSTAASQDLAV"
     gene            complement(2281..3150)
                     /locus_tag="VHM69_17310"
     CDS             complement(2281..3150)
                     /locus_tag="VHM69_17310"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX2742201.1"
                     /translation="MPEAGSLITNPRAGEYRRTTPEPVRHVPTVYHIPVCPFSQRLEI
                     LLTLKGCRHLVDFHVVDITRPRPGWLIEKMHGTTALPVLETEEGGIIKESMVILRYLE
                     DRHPEPAVAQRDPYRRAVEGMMNAMEGGFCALGYRYVLNQDGAKRDEFRRRMLDQYAR
                     LDEFLVRHNPAGTFLFERFGLAETVFTPILMRFWFLEYYEDFDLPREERYARVRRWRE
                     ACLAHPAAQQVSREEIVKVYYDYAKGAGNGSPLPGREHSSFVFAPGWQQRPWPSRDKY
                     GHDATDRELGLVP"
     gene            complement(3284..>3843)
                     /locus_tag="VHM69_17315"
     CDS             complement(3284..>3843)
                     /locus_tag="VHM69_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742202.1"
                     /translation="TNHGQIPGGWFIDPELAGGGAVMDHTVHLADVLRWMLDVEVRSV
                     YAEVDSFFGAEGTDDAAILTLELEGGPIADGTFATIDPSWSRGDGFPTWGDVTLRISG
                     TSGVLDVDPFARPLKTFDHETRAPAWSYTGEDMNALMLADFLRGVAEDDPAGASGLDG
                     LRTLEIVLAAYRSAVDHEPTRISRT"
BASE COUNT          695 a         1257 c         1160 g          731 t
ORIGIN      
        1 gacttcgacc tccgggagct tgcgtccgac gctggatacc cgaagctcca gcgggtcgtc
       61 tgttcgggtc tgggtgatga cgggactaga ctcggtctgc ccgtaggcta tggtgatctc
      121 cgaagcaccc atcacctcaa cgacctgctt catcacctcc atcgggcacg gggaacctgc
      181 catgatccca gtccgcaagc tgctcgtgtc gtattcctcg aagtcagggt gctcgagctc
      241 cgctatgaac atggttggca ccccgagtac cgccgtacac cgctccttgt caactgcctg
      301 caagactttc tccgcgtcga agctctcgac gggaaccatc gtgccctcat gggtaacggt
      361 gttgagcgtc ccgagcacgc agccgaaaca gtggaagaac ggtaccggta tacagaccct
      421 gtcctcgggc ccgagcttca tgcactcccc gatgtggaaa gcgttcttga ctatattggt
      481 gtgggttagc tgtactcctt tcgggaaccc ggtcgtccct gacgtgtact gcatgttgat
      541 gacctcgtcc gcgccgagcg atgcctgcct ctcttcaagc tcctcctgcg agacctcctt
      601 cccgcgctgc atgaactcgt cgaagcccat gatcgggagc ccttcgcgtg agccctcgcc
      661 cattagaacg acatgcctca agtgcggcaa atcctctacc gatagctccc cttctgcgct
      721 ctcggcgagg gcggggactg cctgtccgag gatgtcgacg aagtcggcgc cctttacacc
      781 cgcggttaga aacacggcag cggcgtcgga ttggtcgagt acatacctga gctcgtgcgc
      841 cttgtaagcg gggttcaccg tcacgaggac ggctcccatc ttccccgtcg cgaactgcag
      901 gatcacccac tcggggacgt tctggcccca gacggcgacg tgatctccct tctccaaacc
      961 aagcgccatt agcgcgcggg cgcacctctc aacgacctcc tcgaactccc ggtaggtata
     1021 gcgcaggtcc ctgtcagcgt agacgagggc gtcttcgtca ggccgtcgcc cggccacaag
     1081 atcaaggagc ccgcctacgg tcaggccgtc gatatcctcc gcgcgcattt gttcctcctc
     1141 atgaagtcgt ggtccccacc acggccacga gtgtagcagt cgcgccaatc cgcgcaaccg
     1201 atcaccccga cggcacagac tagacggcga ggtcctggct ggcagcggtg gacccgggag
     1261 gcacgatcac ctttagggcg ttgtggtcga ctgagaaatc ctgcggggtg ctcgttacga
     1321 cttcgccgtc tatgttgatc gggagctccg gctccgtctc gagtaacacc cgctctgttc
     1381 gatggtggtg aacgccctcc atcgggatga aatcaccgct cctggtgtag cggacaaccc
     1441 cgagcagtgt ccttggcctg ccgagctcta tggccaacac gtcgagcctg cggtcgtcta
     1501 tgccggcctc tggggcgagg accatccccc caccatagaa acgcccgttg cctacggcga
     1561 gatgcagaag ccgtccgtac tccaccgtct ccaggtcgcc gtcagggaaa gtgagcctcg
     1621 cggagaaggg ctcgtgctgg aagaaggccc tgatggcggc ggtcggatag gcgagcgccc
     1681 cgatcctctt cagccgggga gtgagggcct gcgtgaccct gacgctcaac cccgtcgagg
     1741 cgacgttcac gaagtagttg tcgtgcacca gtcccaggtc cacgtccacg accctgccat
     1801 tggcgatagt ctggcaagcc tgacctaagt ccaacggaat ctccagcgtc cgcgcgaagt
     1861 cgttggcggt acccaagggg aggaggccga gggtcacgtc gcggttcgcc agaaagtcga
     1921 ccacggagct gaccgaaccg tcgccgccgc ccaggataag gaccctgtaa cgtgggcggt
     1981 cctcgacgat cgcacgcacc gtctcgggaa ggcgtgcggg atcccttata gcgtacgtga
     2041 cgcctagtga gacgtcgagt tcctgtaaac ggtcgagggc cttgaagaac gtccgctccc
     2101 cgctacgcga acgcgtgttg acgatcaaag ccgcgcgctc ctgctcgaag tccgggctca
     2161 cgatgcgtca ctacaagttt cgccgatcgc gtccatcgtc cttgaaatat acgcgactat
     2221 gccgcgatgg tttcgtgcct ggctacaggc acctctcgtt tcgagcaggt tacatagaga
     2281 tcaaggtacg agacccagct cccggtccgt ggcgtcgtga ccgtacttgt ccctggacgg
     2341 ccaggggcgc tgctgccaac cgggtgcgaa cacgaacgag gaatgctcgc gtccagggag
     2401 cggcgagccg ttgcccgcgc ccttcgcgta gtcgtagtag accttcacga tctcctccct
     2461 gctgacttgc tgcgctgctg gatgcgcgag gcacgcctcg cgccacctgc gaaccctggc
     2521 gtacctctcc tcacgcggca ggtcgaagtc ttcgtagtac tccagaaacc agaagcgcat
     2581 aaggatgggc gtgaagacgg tctcagccag cccgaaacgc tcgaagagga aggtgccagc
     2641 ggggttgtgc ctcacgagaa actcgtccag cctggcgtac tgatcgagca tcctccgcct
     2701 gaattcgtca cgtttggcac cgtcctggtt cagtacgtaa cggtagccca acgcgcagaa
     2761 cccgccctcc atcgcgttca tcatgccttc caccgcgcgc cggtaaggat cgcgctgggc
     2821 gacggcgggc tcggggtggc ggtcttcgag gtacctgagg atgaccatgc tctccttgat
     2881 gatcccgcct tcctcagtct cgagcacagg cagagccgtc gttccatgca tcttctcgat
     2941 cagccacccc ggccgcgggc gcgtgatatc cacgacgtgg aagtcgacga ggtgacgaca
     3001 acccttgagg gtaagcagga tctccaaccg ctgggagaat ggacagacgg gtatgtgata
     3061 gacggtcggc acgtgccgca ccggctcggg agtggtccgg cgatactccc cagcacgcgg
     3121 gttggttatc aggcttcctg cttcgggcat cgtgccatcc ttccaatata ttctcgggac
     3181 cggcgcatgg tacccgcacc ccgccgcgaa cgaatcctcc aaacgggcta tttctcgaaa
     3241 acgccggtga ccggcgcgtc gtgccttaga aggcgataga ggtctacgtt cgacttattc
     3301 tcgtgggttc gtggtccacc gccgagcggt atgctgccag gactatttca agggtccgca
     3361 atccatccag cccgctcgct ccggcagggt cgtcttctgc gacgccgcgc aggaagtccg
     3421 cgagcatcag ggcgttcatg tcctctcccg tgtaagacca cgccggagcg cgtgtctcgt
     3481 ggtcgaaagt cttcaagggc cgcgcgaacg ggtccacgtc gaggacacct gaggtgccag
     3541 agatcctgag tgtcacgtcg ccccaggtgg ggaagccatc tccgcgcgac cagctcgggt
     3601 ctatggtggc gaaggtgccg tcggcgatgg ggccgccttc gagctcaagg gtcaggatgg
     3661 ccgcgtcgtc ggtcccctct gccccgaaga acgaatccac ctccgcgtag acgctccgca
     3721 cctcgacgtc cagcatccag cgcagaacgt ccgcgaggtg gaccgtatgg tccatgacgg
     3781 cgccgccgcc ggccagttcg ggatcgatga accagccgcc ggggatctgg ccgtggttcg
     3841 tgc
//
LOCUS       DATAIR010000729         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_261654, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000729 DATAIR010000000
VERSION     DATAIR010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261654"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1224
                     /locus_tag="VHM69_17320"
     CDS             133..1224
                     /locus_tag="VHM69_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742203.1"
                     /translation="MASERKITNLQALISALSTEERTIADRIFDVSTTTGRLVAPESM
                     NAWIEKSFGSIDRVTAQRIVKVTNRITLEGTLFNELRASRPLDTGVSLDLDREVATTG
                     GDPFCNVEVGTPADVFGRVKGEHATTASNIAKYDGFHGVVVFDDHNPLHLTPEKVAGY
                     VSVGLEWCRKAMETDPEARYPFLMWNCLWRAGGSIIHGHAQVTATRGSHYPKVERLRQ
                     VASAYKREHGSDYFKDLGRIHDSLGLGIPLEGVRAFASLSPIKEKELVVIGGDPGDAS
                     LSRAVGALLQGYVHDLGVSSFNVAFYMPPLAPTQEDWSDFPTVVYIVDRGDPANRTSD
                     IGAMELYAASVVASDPFLVSEALRDGAPG"
     gene            complement(1260..1775)
                     /locus_tag="VHM69_17325"
     CDS             complement(1260..1775)
                     /locus_tag="VHM69_17325"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX2742204.1"
                     /translation="MKSWKIAVVALTLLLAGCGGAPANSQNSSQASGTKTPATEETTS
                     ASSDLSTLVIETSGEEQVEVRVEVADNVVEQAKGLMYRKELGEDRGMLFVYPDERVLS
                     FWMRNTLIPLSIAYIDSEGRIVDIQDMKPLDDKPPHYESSEPVQYALEVNQGFFEENG
                     VKEGDHAELPG"
     gene            complement(1772..2482)
                     /locus_tag="VHM69_17330"
     CDS             complement(1772..2482)
                     /locus_tag="VHM69_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX2742205.1"
                     /translation="MTEDLFLKDAYLREFEARVVKLEGREIILDRTAFYPGGGGQPAD
                     KGSLGIGPVRANVVDARREGNEIVHVLDRAIPDTVRDLKGALDWERRFAHMRYHTALH
                     VLSGVIWRNFDAKVTGGQMRSDRARMDFSFPGEWTTGVVGEIERLTNEALAEERPVRV
                     YELEREGALANPDLIRTQVNLVPERVRTIRIVEIEGLDTQADGGTHVANTSEVGEMEI
                     TGHKSKGRHNKRIEFVLR"
     gene            complement(2479..>3008)
                     /locus_tag="VHM69_17335"
     CDS             complement(2479..>3008)
                     /locus_tag="VHM69_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2742206.1"
                     /translation="EERMEHDATALPYDYVIGSVHRVDNEEVDRATDQRIYDRYETYD
                     LYAAYYANVRKAALSGRFEILGHPDLIKIFRHFPERDITPILEETADAVAESGIVVDV
                     NAAGLRKPIGEIYPARNFLEMFHRRGVPIILSSDAHAPDEVAAGYDTSLKLVHDVGYR
                     EVATFKNHERGTLPL"
BASE COUNT          541 a          986 c          904 g          577 t
ORIGIN      
        1 cccgaggccg cccgcttcgc ccacgatgtc gcgagcctga agctgaaagt cgaggggcac
       61 gtggaacgta tgatcgaccg cgagaaagtc tacgcaggtc tacaagacgc cgaacagcgg
      121 gcacgatagc ctgtggcctc cgaacgcaag ataacgaacc ttcaagccct gatctccgcc
      181 ctctcaacgg aagaacgcac catcgccgac cgcatcttcg acgtatcgac gacgacggga
      241 cgcctcgtcg cacccgaatc catgaacgcc tggatcgaga agtccttcgg ctcgattgac
      301 agggtcacgg cccagcgcat cgtgaaggtg acgaaccgca tcaccctgga aggcaccctc
      361 ttcaacgagc tgcgagcctc ccgcccactc gacaccggcg tgagcctcga cctcgacagg
      421 gaggtagcaa cgaccggagg cgacccattc tgcaatgtgg aagtcggcac gccggctgac
      481 gtcttcggcc gcgtgaaggg cgagcacgcg accacagcct cgaacatcgc caagtacgac
      541 ggcttccacg gcgtcgtcgt cttcgacgac cacaaccccc tccacctcac gccggagaag
      601 gtcgcaggct acgtctccgt cggcctggaa tggtgccgca aggccatgga aaccgacccc
      661 gaggcacgct accccttcct gatgtggaac tgtctctgga gggccggggg ctccatcatc
      721 cacggccacg cgcaggtcac ggccacccga ggctcccact atcccaaggt cgagcgcctg
      781 cgccaggtcg cctccgccta caaacgagaa cacggctccg actacttcaa ggatctcggc
      841 cgcatccatg actcgcttgg cctcggcatc ccgctcgaag gcgtcagggc gttcgcgagc
      901 ctctccccca tcaaggagaa agagctggtg gtgatcggcg gcgaccccgg agacgcttcc
      961 ctgagccgcg cggtcggagc cctgctgcaa ggctacgtcc acgacctcgg cgtcagcagc
     1021 ttcaacgtcg ccttctacat gcccccactc gccccaacac aggaggactg gtcggacttc
     1081 cccaccgtcg tctatatcgt tgacagggga gatccagcaa accgtacttc ggatataggg
     1141 gcgatggaac tctacgcagc ctccgtcgtg gcctccgatc ccttcctggt gtccgaagct
     1201 ctgcgtgacg gcgcacccgg atagcatcaa cgttataccc tcgtcggggc tgcgggagcc
     1261 taccccggta actcggcgtg gtccccttct ttcacgccgt tttcctcgaa gaagccctga
     1321 ttgacctcga gggcgtactg gaccggttcg gacgactcat agtggggcgg tttgtcgtcc
     1381 aggggcttca tgtcctggat gtcgacgatg cgtccctccg agtcgatgta ggctatggag
     1441 agcggtatga gggtgttcct catccagaat gagagcaccc tctcgtccgg gtagacgaag
     1501 agcatgcccc gatcctcacc cagctcctta cgatacatga ggcccttcgc ctgctcgacg
     1561 acgttgtcgg ccacctcgac ccgcacctcg acctgctctt ctccagaagt ttcgatgacc
     1621 agagtactga ggtccgatga ggctgaggtc gtctcttcgg tcgctggggt cttggtgccg
     1681 gaagcctgag aagaattctg cgagttggcc ggcgcgccgc cgcatcctgc gaggagcagg
     1741 gtgagagcta cgactgctat cttccaagat ttcatctgag cacgaactct atccgcttgt
     1801 tatggcggcc cttgctcttg tggcctgtga tctccatctc ccccacctcg ctggtattgg
     1861 cgacgtgcgt gcctccgtcg gcctgcgtat cgaggccttc gatctccacg atccgaatag
     1921 tccgtacccg ctcggggacg aggttgacct gggtacggat gaggtcgggg ttcgcgagcg
     1981 ccccctcgcg ctccagctcg tagaccctga caggacgttc ttcggccagg gcctcgttcg
     2041 tcagacgttc tatctcgccg acgaccccgg tcgtccactc gcctgggaat gagaaatcca
     2101 tcctggcgcg gtcggagcgc atctggccgc ccgtgacctt cgcgtcgaag ttgcgccaga
     2161 tcacacctga gaggacgtgc aaagccgtgt ggtagcgcat gtgcgcgaac cgccgctccc
     2221 agtcaagcgc gcccttcaga tcccgcaccg tatcagggat ggctctgtcc aggacgtgga
     2281 cgatctcgtt accctcccgt ctcgcatcaa cgacgtttgc cctgacagga cctatgccca
     2341 gagacccctt gtccgcgggc tggcctccgc cgccgggata gaaagcggta cggtcgagaa
     2401 taatctcacg gccttccagc tttacgacgc gggcctcgaa ctcacgcaga taagcatcct
     2461 tcaggaagag gtcttctgtc acagtgggag ggttccccgc tcgtggttct tgaaggtggc
     2521 gacctcgcgg tagccgacgt cgtggacgag cttcaggctc gtgtcgtagc ctgctgcgac
     2581 ctcgtcgggg gcgtgggcgt cggaggagag gattatgggg acacccctgc ggtggaacat
     2641 ctcaaggaag ttcctggcgg gatagatctc gccgatgggc ttcctcagac ccgcggcgtt
     2701 cacatcgacg acgatgccgg actcggctac ggcgtcggcg gtctcctcca ggatcggggt
     2761 gatgtcccgc tcggggaagt ggcggaagat ctttatgagg tcggggtggc caaggatctc
     2821 gaaccttccc gagagggcgg ctttcctgac gttggcgtag tacgcggcgt agaggtcgta
     2881 ggtctcgtac ctgtcgtaga tcctctggtc agtggccctg tccacctcct cgttatccac
     2941 cctgtggacg ctgccgatga cgtagtcgta ggggagggca gtggcgtcgt gttccatccg
     3001 ctcctcgg
//
LOCUS       DATAIR010000730         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_215938, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000730 DATAIR010000000
VERSION     DATAIR010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215938"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1867
                     /locus_tag="VHM69_17340"
     CDS             209..1867
                     /locus_tag="VHM69_17340"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX2742207.1"
                     /translation="MSSEADRIRVCQWGTSDANNYGDRLFPLIAREQLKARLPGLDMT
                     CFAPIGNATLPPGSPPLFPLFQDGESLDRQRREYFARNFDAVLIGGGDVLRFDRSESG
                     YGDHEHEGPLRPYDAFLDFSWAETVPNILWNAPGVPFPFEPARRMLVRLARSHVRYAA
                     VRDEVSRGHLLEAGVEEPVHVYPDSGILLAEIIRQRVSREKVSDLLATWGAGSNGRGL
                     LCFQCSPGFLRRGEELIAKSLARIAARRDLDVVLLPIGLCHGDQDALRLVQRASGDRF
                     TLVEDLEAPLEIGAVIGACDYFVGSSLHGNLTALSFGIPHLVVNNSVPAVKLDGLLQM
                     ARLEEFRIVDWGDLEDGFDRLAAAPRERWAKAGDRLKILASEHFDRMAHEISRSAAER
                     RAPDGNGSRPRQATAQDFPLEVYDMIAGVHEQLDDERAARRASEREMANRLREMGDRL
                     RSQRSRYEARVELLEARVEHFKKRGEELEARARDLVTRLQDLKVQNANLKARNEDLSG
                     QLRDIEQSTTWRIFGPYRRLRESVGRLTRTSPEDAKEHSATRSE"
     gene            1978..3057
                     /locus_tag="VHM69_17345"
     CDS             1978..3057
                     /locus_tag="VHM69_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2742208.1"
                     /translation="MIKNSVGIWAFGPAVTRFVPPGYHHEVADEPMVEKTERVAGGLH
                     DLLEGLEYHYPGEINEDNAEAVLKVLAAHGMDLPVIAAGLHPDPTYAMGAFINPDAGL
                     RRRAIDTLRRGIDLCSEIGANFIIWPGAEGYNYTFQRPYAETWRHFVEGVAELTDHAN
                     EKDVKVFLEHKNSEPAMKILMQNIGMTLFTIQKVNALGVDTTHLMVNMDWQHLIMNGE
                     NLAEYADLLAAEHKLGHQHGNDGWGTFDDDNVVGTNFFMQILELAQTLQDVGYGEEGE
                     IIGFDLYPYTEDQVAAVRRAIVQWEFIWDLAKKIDRAELREARNRGDALASQKAVYAA
                     LGMGGDYEAEVASRRKEARAGRSEA"
     gene            3054..4544
                     /gene="xylB"
                     /locus_tag="VHM69_17350"
     CDS             3054..4544
                     /gene="xylB"
                     /locus_tag="VHM69_17350"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX2742209.1"
                     /translation="MSVLIGLDVGTGGARAVAVDESGSVVAEASSAYPLQSPRPGWTE
                     QDPDDWWRGAREALGKVAAEEEVAGIGLTGQMHGSVFLDSSDEVIRPALLWNDQRTYR
                     QCDEITRIVGEERLISIAGNPALTGFQAPKIVWLRDEEPANFGQVASVLLPKDYVRLQ
                     LTGEYATDASDAAGTLLLDVRARDWSREILDALEIPHEWMPEVYEGPENTGTLREEVA
                     EQLGLPAGIPVAAGGGDNAAAAVGTGIVGPGLVSSSVGTSGVLFAHASEFDPDPSGRL
                     HAFCHAVPGAYHLMGVTLSAGGSLSWWRETLGGDYDELVEAASEVPPGAEGLLFLPYL
                     SGERTPHLDPKARGAFFGLTSRHGVAHMTRAVMEGVIFSLRDSLEIMRELEVPVEDVR
                     ATGGGARSALWRRLQADIYGTPVRRTVADEGPAYGAALLAGVAAGAYSDVDEATSVVK
                     MREEITEPERERAKVYEEHYEVYRSLYPATSQAMSLLTDLAAGSAG"
     gene            4579..>5643
                     /locus_tag="VHM69_17355"
     CDS             4579..>5643
                     /locus_tag="VHM69_17355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742210.1"
                     /translation="MSRNAGGSYPGFAENAVEDEALDYRPVLLAVAVATVGVLPAFLT
                     GGLVVQIRSELDFGSAALGLAVAVFFASASLASVVMGRVVEKIGAHRGMRLSAVASAA
                     SLLGVALFAGSWEGLVACLVLGGLANAVAQPATNLSLARGVPAGRQGVSFGIKQAAIP
                     TATLLAGLAVPGIAVTLGWRWAFAGGAALALLIALLVPAESLGGVRRLKEARAGDVSV
                     APLVLLALGIGLGSTAATPLGAFIVESSVASGLNVGTAGLLLALGSAAGIVVRVWFGH
                     LADGMGGGRLRLVAAMLGVGVAGFVMLASGKVGLILPATLLAFGAGWGWPGLFNFAVV
                     KTNPGAPAAATGITQTGASGG"
BASE COUNT          975 a         1744 c         1964 g          960 t
ORIGIN      
        1 cgggtggccg ccttcctggc ttccccggcc aacagctacg ttacggggca ggcgatcctg
       61 gtggacggtg gcatggtcag ggcgatatga tctgcccgga tccggtgacg taaaccatgc
      121 gaggtcagtt tgtggagaaa cacatctctc gcaataaaga aggtatctcc ccacgggcgt
      181 acgggggacg ccggaggctt cggtcgagat gagtagcgag gctgacagga tcagggtgtg
      241 ccagtggggg acttccgacg cgaataacta cggggatcgg ctcttccccc tgatcgccag
      301 ggagcagttg aaggcgcgtc taccaggcct ggatatgacg tgcttcgcgc caattggcaa
      361 tgccaccctt ccaccgggga gcccgccgct ctttcctctg tttcaggacg gcgaatcgct
      421 ggaccggcag cggcgagagt acttcgccag gaacttcgac gccgtactca tcggcggtgg
      481 agacgttctg cgcttcgacc gctccgagtc cggctacggt gaccatgagc acgagggtcc
      541 tctgcgtccg tacgatgcgt ttttggactt ctcatgggcg gagaccgtac cgaatatcct
      601 ctggaacgcg ccaggggtgc cctttccatt cgagcccgcc aggcggatgc tggtacggct
      661 ggcgcgctct cacgtaaggt acgcggcggt gagggacgag gtgtcgcgag ggcacctgtt
      721 ggaggccggg gtagaagaac cggtgcacgt gtacccggac agcggcattt tgttggcgga
      781 gatcattcgt cagcgtgttt ccagggagaa ggtcagcgac ctgctggcta catggggcgc
      841 cgggtcgaac ggtcgggggt tgctctgctt tcagtgctct cccggtttct tgaggcgcgg
      901 ggaggagctg atcgcgaagt ccctcgccag aatagccgcc aggcgagacc tcgacgtcgt
      961 cttgctcccg atagggctgt gccacgggga tcaggacgcg ctccggctgg ttcagagggc
     1021 gagcggggac cgcttcaccc tggtggagga cctggaggcg cctctggaga tcggggcggt
     1081 aataggggcc tgcgattact ttgtcgggag tagcctgcac ggtaacctga ccgccctttc
     1141 gttcgggatt ccccacctcg tcgtgaacaa ttccgtgccc gccgtgaagc tcgatggttt
     1201 gctccagatg gcccggctcg aggagttccg tatcgtcgac tggggggatc tggaggacgg
     1261 cttcgaccgg ctggcagccg cgcccaggga gcgttgggcg aaggctggag accgtctcaa
     1321 gatactggca tccgagcatt tcgaccgaat ggctcatgag atatcgcgct ccgccgcgga
     1381 gcgccgcgcg cccgatggta atggctcgag accgcgccag gcaacagccc aagacttccc
     1441 tcttgaggtg tacgatatga tagccggagt gcacgagcaa ctggacgacg agagggccgc
     1501 ccggcgcgca agtgaacggg agatggccaa caggctgcgt gagatgggcg acaggctccg
     1561 ctcgcagcgg tcgcgttacg aggccagggt cgaactactc gaagcccgcg tcgaacactt
     1621 caagaagcgg ggcgaagagc tggaagccag agcccgagac ctcgtgaccc ggctgcaaga
     1681 tctcaaggtc cagaacgcga accttaaggc caggaacgag gacctgagcg gccagctccg
     1741 agatatagaa cagtccacga cctggcggat tttcggaccc taccggaggc tgcgcgagag
     1801 tgtaggcagg ctgacgcgga catccccaga ggatgcgaag gaacactctg ccacacgctc
     1861 cgagtagacg gtcacaggtg ccatggtccg gtactctaaa aagcgagcgc ctgtagtgcg
     1921 gatcgggcta tgatgagcgg agggctcgca cgtcctttct agggaaggag gatctggatg
     1981 atcaagaact cggtgggtat ctgggctttt ggtcctgccg tgacgcgctt cgtgccgccg
     2041 ggctaccacc acgaggtggc tgacgagccg atggtcgaga agacggaacg cgtcgccggt
     2101 gggctccacg acctccttga ggggctggag tatcactacc cgggtgagat caacgaggac
     2161 aacgcagaag cggtgctgaa ggtcctcgcc gcccacggga tggatctgcc cgtgatcgcc
     2221 gctggcctcc atcccgaccc gacgtacgcg atgggcgcgt ttatcaaccc ggacgcaggg
     2281 ctaagacgcc gggccataga caccctcagg cgcggcatag acctgtgctc ggagatcggg
     2341 gcgaacttca tcatctggcc aggcgccgaa ggctacaact acaccttcca gcgtccttac
     2401 gctgagacct ggcggcattt cgtcgagggt gtagccgagc tgaccgacca cgccaatgag
     2461 aaagacgtca aggtatttct cgagcacaag aactccgagc cagcgatgaa gatcctgatg
     2521 cagaacatcg gcatgacgct cttcaccatc cagaaggtca acgccctcgg ggtggatacg
     2581 acccacctca tggtcaacat ggactggcag cacctcataa tgaacggcga gaatctggcc
     2641 gagtacgccg acctccttgc ggcggagcac aagttgggcc accaacacgg caacgacggc
     2701 tggggcacct tcgacgacga taacgtggta ggcacgaact tcttcatgca gatcctggag
     2761 ctggcccaga ccctccagga cgtcggctac ggggaggagg gcgagatcat cggctttgac
     2821 ctctacccgt acacggagga ccaggtggcc gccgtgaggc gcgccatcgt gcagtgggag
     2881 ttcatctggg acctggcgaa gaagatagac agggccgagc tgcgcgaggc caggaatcgg
     2941 ggcgacgccc tggcgagcca gaaggcggtc tacgccgcac taggcatggg cggcgactac
     3001 gaagccgagg tcgccagccg ccgcaaggag gcgcgcgccg gccggagtga ggcgtgagcg
     3061 tcctcatcgg tctcgatgtc gggacaggcg gcgcgagggc agtcgccgtg gacgagtccg
     3121 ggagcgtcgt cgccgaagcc tcttcagcgt acccgctaca aagcccgcgc ccaggctgga
     3181 cagagcaaga ccccgacgac tggtggaggg gagccagaga agcgctcggg aaggtcgccg
     3241 ccgaagagga ggtcgcgggg atcgggctca cggggcagat gcacggctct gtgttcctcg
     3301 attcgtctga cgaggtgata cggcccgcgc tgttgtggaa cgaccagcgc acctacaggc
     3361 agtgcgacga gataacgcgg atagtaggcg aagaacggct gatctccatc gcgggcaacc
     3421 ccgccctcac gggcttccag gcgcctaaga tcgtctggct cagagacgaa gagccagcga
     3481 acttcgggca ggtcgccagc gtgctcctgc caaaagacta cgtacgactc cagctcacgg
     3541 gcgagtacgc aacggatgcc tcagacgccg cgggcaccct gctcctcgac gtacgggccc
     3601 gcgactggtc gcgtgagatc ctggacgccc tagagatccc gcacgaatgg atgcctgaag
     3661 tctacgaagg ccccgagaac accggcactc tccgtgaaga agtggccgag cagcttggcc
     3721 tgcccgctgg catccccgtt gccgctggcg gcggcgacaa cgccgctgcg gctgtaggga
     3781 cgggcatagt cggccccggg ctcgtcagct cctccgtagg gacgagtggc gtcctcttcg
     3841 cccacgcaag cgagttcgat cccgacccgt cggggcgtct tcacgccttc tgccacgcgg
     3901 tgcctggcgc ctatcatctg atgggcgtca cgctctccgc cggcggctcc ctctcctggt
     3961 ggcgagagac gctcgggggc gactacgacg agctggtcga ggcggcctca gaggttccgc
     4021 ctggcgccga ggggcttctc ttcttgcctt acctctccgg agagcggacg ccgcacctcg
     4081 accccaaggc aaggggtgct ttcttcgggc tcacgagtcg tcacggcgtg gcgcacatga
     4141 cacgcgccgt gatggagggt gtgatcttct ccctgcgtga ctccctggag atcatgcgcg
     4201 agctcgaagt gcccgtagag gacgtgcggg cgaccggcgg tggggcgagg agcgccctct
     4261 ggcgccgact tcaggccgac atctacggca ccccggtgcg ccgcaccgtc gccgacgagg
     4321 ggccggccta cggtgcagcg ctcctggcag gggtggccgc gggtgcgtac tccgacgtgg
     4381 acgaagcgac ctccgtggtg aagatgcgag aggagattac cgagccggag agggaacggg
     4441 cgaaggtata cgaggagcac tacgaagtct accgctcgct gtaccctgcg acgagtcagg
     4501 cgatgtccct tctcaccgat ctcgcggctg gctccgccgg ctgaggggtc tcgcaagccg
     4561 ggatggtaag atctcggcat gtctcgtaac gcggggggat cataccccgg cttcgcggaa
     4621 aatgctgtag aagatgaggc actagactat cgtcccgtgc tgctcgccgt ggctgtcgcc
     4681 accgttggcg tgctccctgc cttcctgacc ggggggctcg tggtccagat ccggtccgag
     4741 ctggacttcg ggtctgcggc gcttggtctg gccgtcgctg tgttcttcgc ctcggcctcg
     4801 ctcgcctctg tggtcatggg gcgcgtggtg gagaagatcg gagcccaccg tgggatgcga
     4861 ctctcagcgg tggcgagcgc ggcctcgctg ctcggcgtcg ccctcttcgc cgggtcctgg
     4921 gaagggctcg tggcctgcct ggtgctcggc gggctggcca acgcggtcgc ccagccggcg
     4981 acgaacctct cgctggcccg aggggtcccc gcgggaaggc agggggtgtc gttcgggatc
     5041 aaacaggcag ccataccgac ggcgaccctg ctcgccggcc tcgccgtgcc aggcatcgcc
     5101 gtcacgttgg gttggcgctg ggctttcgcg ggaggcgccg cgctcgcgct tctcatcgcc
     5161 ctgctcgtgc ccgccgagag cctcggcggc gtgaggcggc tcaaggaggc gcgggccggg
     5221 gacgtgtccg tcgcgcccct cgtcttgctc gccctcggca tcgggcttgg atctacggcc
     5281 gcgacaccgc tcggagcttt tatcgtggag tcttctgtcg cctcggggct gaacgtcggg
     5341 acggcggggc tcctgctcgc cctcgggagc gcggccggga tcgttgtgcg cgtgtggttc
     5401 gggcatctcg ccgacggtat gggcggtgga cgtctccggc tcgtcgccgc gatgctcggg
     5461 gtcggcgtgg cagggttcgt gatgctcgcc agcgggaagg tcggcctcat cttgcctgcc
     5521 accctgctcg cattcggggc gggatggggg tggcccggcc tcttcaactt cgccgtcgtc
     5581 aagaccaacc ccggcgcgcc ggcggcggcc actgggatca cgcagaccgg cgcctccgga
     5641 ggc
//
LOCUS       DATAIR010000731         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_71567, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000731 DATAIR010000000
VERSION     DATAIR010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71567"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHM69_17360"
     CDS             <1..318
                     /locus_tag="VHM69_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX2742211.1"
                     /translation="DRIYHFHIKDSRKRLNGRRSILGGHLDFGEPERGWDFVSPGHGD
                     VNLEDCIRALNRIGYEGPLSIEWEDSGMDREYGAQDALAFARRTDFEPSTQAFDAAFA
                     REE"
     gene            321..1493
                     /locus_tag="VHM69_17365"
     CDS             321..1493
                     /locus_tag="VHM69_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742212.1"
                     /translation="MEKREIGVGLVGYKFMGRAHSNAYRQVARFFDVDPAPHMAAICG
                     RDEAGVKEAAENLGWESYETDYRELIARDDVDLVDVVTPGNTHKEIVLAALEAGKHVL
                     CEKPLANSLSEAREMVTAARNAGTVNMVCFNYRRAPAVQLARKLIEEGRLGEIRHWRA
                     TYLQDFIMDPSFPLIWRLKKEVSGSGALGDIGAHIIDLAQFVVGPISEVTGMTETFIK
                     ERPLEEASAGGGLSASGGTETGEVTVDDAAAFLARFENGAIGTFEATRFAAGRRNQNS
                     FEVNGSKGSVAFDLERMNELEVFFTGDDADVQGFRTVNVTEPDHPYTGAWWPPGHILG
                     YEHTFIHTIKDLMDGIKRGESPAPTFEDGYRCQAVLDAVERSAASGEWKKPQMEGI"
     gene            1496..2206
                     /locus_tag="VHM69_17370"
     CDS             1496..2206
                     /locus_tag="VHM69_17370"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX2742213.1"
                     /translation="MADTPRVTIWNEYRHEKSDENVAKIYPEGIHGALAAMLTEGGFE
                     ASTATLDEPEHGLTDEVLNTTDVLLWWGHMAHDEVSDEIVEKVHDRVLGGMGLIVLHS
                     GHFSKIFKKLTGTTCDLKWRHDDKERLWVVAPGHPIAEGLGEYIELEEEMYGEPFDVP
                     QPDELVFVSWFGGGEVFRSGLCYRRGRGRIFYFRPGHEENPSYHNPDVQLVIRNAVRW
                     ASPTPGATPVYGHTEPMD"
     gene            2240..3355
                     /locus_tag="VHM69_17375"
     CDS             2240..3355
                     /locus_tag="VHM69_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742214.1"
                     /translation="MQPETSFSKPLRAGVIGLGYAGEQHLKNYQRIPGVEAVALAGLE
                     EDILGELGGRYGVPDLYTSWEDLVARDDLDIVSIGAPNHLHAPIAVAALEGGRHVLCE
                     KPLARTGVEARRIVNAAREADRAVHIAFTQRERGDVQALKRHVDEGNLGRIYHAKATW
                     MRRNGIPGMGGWFTSKELAGGGPLIDLGVHMIDMALYLMGEPEVETVSCATYAELGPR
                     GRGGRGEFGLMQGDKPYEVEDLATAFIRLSGGATLNLEAGWAAYRESSDDFGVTLYGT
                     DGGAEMKVKNYGTSDTVRIYTDVAGFPAVVTPEIAPREGHYAVVRRFVETIRGGNWEG
                     QFGEDGLNRARIIDACYASALENREVSLDEISREEAV"
     gene            3352..>3697
                     /locus_tag="VHM69_17380"
     CDS             3352..>3697
                     /locus_tag="VHM69_17380"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX2742215.1"
                     /translation="MKNALFVCGGWEGHEPEKCARIFAPFLEEKGYGVEISTTLDSYL
                     DEEKMRSLDLVVPIWTMGTITDEQETGLLDAIEGGVGLAGWHGGMADSFRNNIKYQFM
                     VGGQWVAHPGNII"
BASE COUNT          754 a         1136 c         1240 g          567 t
ORIGIN      
        1 gacaggatct accacttcca catcaaggac agccgcaagc ggttgaacgg ccgcaggagc
       61 attctcggag gccacctcga cttcggcgaa cccgagcgcg gctgggactt cgtctcgccg
      121 ggccacggcg acgtgaacct ggaggactgc atcagagccc tgaaccgtat cggctacgaa
      181 ggcccgctta gtatcgagtg ggaggactcg gggatggacc gcgagtacgg cgcccaggac
      241 gccctggcgt tcgcgaggcg cacggacttc gagccatcga cacaggcgtt cgacgccgcg
      301 ttcgcgaggg aggagtagag atggagaagc gcgagatcgg ggtcggactc gtcggctaca
      361 agttcatggg ccgcgcccac tccaacgcgt accgtcaggt cgcccgcttc ttcgacgtgg
      421 acccggcccc gcacatggcc gccatatgcg gacgcgacga ggccggcgtc aaggaggcag
      481 cggagaacct cggatgggag tcctacgaga ccgactaccg cgagctcata gcccgcgacg
      541 acgtggacct ggtggacgta gtgacaccgg gcaacactca caaggagatc gtgctggccg
      601 ctctggaggc gggcaagcac gtcctttgtg agaagccgct cgcaaattct ctatcagagg
      661 cacgtgagat ggtcacggcg gcgcgcaacg ccggcacggt caacatggtc tgcttcaatt
      721 acaggcgggc gcctgccgtg cagctcgcga ggaagctcat cgaggaaggg cggctgggtg
      781 agatccggca ctggcgggcg acttatttgc aggacttcat catggaccct tcgttccccc
      841 tgatctggcg cctcaagaaa gaggtctcgg gctcaggcgc cctgggcgat ataggcgccc
      901 acatcatcga cctcgcccag ttcgtcgtcg gccctatctc cgaggtcaca gggatgacgg
      961 agacgttcat caaggagcgg ccgctcgaag aggcgagcgc cggcggcggc ctctccgcga
     1021 gcggaggcac tgagaccgga gaggtaacgg tggacgacgc cgcggccttc ctggcccgct
     1081 tcgagaacgg cgccatcggg accttcgagg cgacgcgctt cgccgctggc cgcaggaacc
     1141 agaactcctt cgaggtcaac ggctcgaagg gcagcgtcgc cttcgacctg gagcggatga
     1201 acgagctcga agtcttcttt accggggacg atgccgatgt acagggcttc cgtaccgtga
     1261 acgtgacgga gccggaccat ccctacacgg gagcgtggtg gccgccggga cacatcctcg
     1321 gctacgagca caccttcatc cacaccataa aggacctgat ggacggtata aagaggggcg
     1381 agagcccggc gccgacgttc gaggacggct accgttgcca ggccgtcctc gacgccgtcg
     1441 agcgcagcgc cgcgagcggc gaatggaaga aaccacagat ggaagggata tagacttggc
     1501 ggacacgccg cgcgtaacta tctggaacga gtacaggcac gagaagagcg acgagaatgt
     1561 cgcgaagata tatccggaag gcatccacgg cgcgctcgcc gcgatgctca ccgaaggcgg
     1621 cttcgaggcc agtacggcca ccctcgacga gcccgaacac ggtttgacgg atgaagtcct
     1681 gaatacgacg gacgtgctcc tctggtgggg gcacatggcc cacgacgagg tctcggacga
     1741 gatagtggag aaggtgcacg atcgtgtgct cggcggcatg ggcctgatcg tgctccactc
     1801 cgggcacttc tcgaagatct tcaagaagct cacgggcacg acctgcgacc tcaagtggcg
     1861 ccacgacgac aaggagaggc tctgggtcgt cgcgccaggc catcccattg ccgaggggct
     1921 cggcgagtac atagagctcg aagaagagat gtacggtgag cccttcgacg tgccgcagcc
     1981 agacgagctc gtcttcgtga gctggttcgg cgggggggag gtctttagga gcgggctctg
     2041 ctacaggcgg ggtcggggca ggatcttcta cttccgaccg ggccacgagg agaacccctc
     2101 ctaccacaac cccgacgtgc agctcgtcat ccgaaacgcc gtccgctggg catcgcctac
     2161 gccaggggcg acgcctgttt acggacacac ggagccgatg gactagagta agaccacata
     2221 cctgtcagag gagagcaatt tgcagcctga aacttcattc tcaaaacccc tgcgcgccgg
     2281 ggttatcggc cttggatacg ccggcgagca gcacctgaag aactatcaga ggataccggg
     2341 cgtcgaggct gtcgcactcg ccgggctcga agaggacatc ctcggcgagc tcggaggacg
     2401 ctacggcgtg cccgaccttt acacgagctg ggaggacctc gtcgcccgcg acgacctcga
     2461 catcgtgagc atcggggctc cgaaccacct gcacgcaccc atcgccgtcg ccgccctcga
     2521 agggggcaga cacgtactct gcgagaaacc gctcgcccgt acgggggtcg aggcccggcg
     2581 tatagtaaac gccgccagag aggcggatag agcggtacat atcgcgttca cccagcgcga
     2641 gcggggcgac gtgcaggcgc tcaagcggca cgtcgatgag gggaacctgg gcaggatcta
     2701 ccacgccaag gcgacctgga tgcgccgcaa cggcatccct ggtatgggcg gctggttcac
     2761 gagcaaggaa ctcgcgggcg gaggccccct catagacctt ggggtgcaca tgatcgacat
     2821 ggccctctac ctgatgggcg agcccgaggt cgagaccgtg agctgcgcaa cctacgccga
     2881 gcttggcccg cgtggcaggg gcggaagggg tgaattcggt ctcatgcagg gcgacaaacc
     2941 atacgaggtc gaggacctgg cgacggcctt catacgcctc tccggtgggg ctacgctcaa
     3001 cctcgaggca ggttgggccg cataccgcga gtcgagcgac gacttcggcg tcaccctgta
     3061 cggtaccgac gggggcgcgg agatgaaggt caagaactac ggcactagcg acaccgtccg
     3121 catctacacc gacgtggccg gcttccccgc cgtagtcacg cccgagatcg caccccgcga
     3181 gggacactac gctgtggtcc gacgtttcgt cgagacgatc aggggcggaa actgggaggg
     3241 gcagttcggg gaggacggcc tgaaccgcgc ccgcatcata gacgcctgct acgcctcggc
     3301 ccttgaaaat cgcgaggtct ccctcgatga gatatccaga gaggaagccg tgtgaagaac
     3361 gcgctcttcg tctgcggtgg ctgggagggc cacgagcccg agaagtgcgc acggatcttc
     3421 gcgccgttct tggaggaaaa agggtacggg gtcgagatct cgaccaccct cgactcctac
     3481 ctcgacgagg agaagatgcg ctctctcgac cttgtggtcc cgatctggac aatggggacc
     3541 atcacggacg agcaggagac gggccttctt gatgcgatag agggcggggt cggtctcgca
     3601 ggctggcacg gcggcatggc cgactcgttc cgcaataaca tcaaatacca gttcatggtc
     3661 gggggacagt gggtcgccca tcccggcaac atcatcg
//
LOCUS       DATAIR010000732         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_71659, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000732 DATAIR010000000
VERSION     DATAIR010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71659"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VHM69_17385"
     CDS             complement(<1..608)
                     /locus_tag="VHM69_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742216.1"
                     /translation="MAKIKIDLDRGIGELDRRIFGGFIEHLGRCIYGGVFEEGSPLSD
                     DRGYRLDVLEAAKGLRIPILRWPGGNFVSGYHWTDGIGPREERPRKTELAWFTEESNR
                     FGTDEFIEYCRLLDTEPYICVNMGTGTMDEAQAWVEYCNGTGDTYWANLRRENGREEP
                     YNVKYWGLGNEMYGEWQIGALSADDYVRKAREFAKVMRWTDP"
     gene            complement(568..1632)
                     /locus_tag="VHM69_17390"
     CDS             complement(568..1632)
                     /locus_tag="VHM69_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2742217.1"
                     /translation="MRDVASKAGVSFQTAGKVLNGKGTVSPETRERILEAANALGYVP
                     NALARSLVTRSTCTIGIVASDLSDAELARFVVGAEREARRQGHGVLIGSIDQEGTDAA
                     RYLRMLLERRVDGILTAAPQMEHDARIGDLLRGPVPAVSIHRIPGGGVPLVGSDHVET
                     SLLATRHLISLGHRRIATITGSQDRQVTSMRLKGYERALEEAGVSYDPTLVESGNWEA
                     DGAYDATLRLLDRVSDITAFYAQNDTMALGVLSALYERGLGVPDDCAVVGCDDIPMAA
                     HAIPPLTTVHVPFYETGEIAVRLLLDVIAGRADRPKRVLLPVRLVRRSSCGEESDRHA
                     ISQGGVKGGEDQNRPRPRDR"
BASE COUNT          297 a          609 c          523 g          337 t
ORIGIN      
        1 ttcgggtccg tccacctcat caccttggcg aactcacggg cctttctcac atagtcatcg
       61 gccgagagag ccccgatctg ccactctccg tacatctcgt tgcccagtcc ccagtacttg
      121 acgttgtaag gctcctcgcg gccgttctcg cgcctgaggt tggcccagta agtatcgccg
      181 gtaccgttgc agtactcgac ccaggcctgc gcctcgtcca tcgtaccagt gcccatattg
      241 acgcagatgt aaggttcggt atcgagcagg cggcagtact cgatgaactc atcggtaccg
      301 aagcggtttg actcctccgt gaaccaggcg agctccgtct tcctgggacg ctcctcgcgc
      361 gggccgatcc catccgtcca gtggtaaccg ctgacgaagt tgccgccggg ccagcgcagg
      421 atggggatgc gcaacccctt cgcggcctcg agaacgtcta gcctgtagcc tctatcgtcg
      481 ctcagaggtg acccctcttc gaagaccccg ccgtagatac agcggccgag gtgctcgata
      541 aaccctccga agattcgcct gtccagctca ccgatcccgc ggtcgaggtc gattttgatc
      601 ttcgccacct ttcacccctc cctgggagat tgcatgcctg tcactttctt ctccgcacga
      661 gcttcggcgc accagccgga ccggcaagag tacgcgcttc ggcctgtcgg ccctgcctgc
      721 gatgacgtcg agcaggagac gaacggcaat ctcccccgtc tcgtagaagg gcacgtgcac
      781 tgtggtaagc ggcgggatcg cgtgcgcggc catggggatg tcgtcgcacc ctacgaccgc
      841 gcaatcatcc ggcaccccga gcccccgctc gtacagcgcg ctcaacacgc ctagtgccat
      901 ggtgtcgttc tgcgcgtaga aagcggtgat atcggatact cggtccagta gccgcaacgt
      961 cgcgtcgtac gccccgtccg cctcccagtt cccgctttcg acgagggttg ggtcgtaaga
     1021 aacgccggcc tcttcgagcg cgcgctcgta gcccttcagc cgcatggagg tgacctgacg
     1081 atcctgtgag cctgtgatcg tcgctatgcg cctgtgcccc aggctgatca ggtgtcgggt
     1141 cgcaagcaag gacgtctcga cgtggtcgga gccgacgagc gggacgcctc cgccgggaat
     1201 ccggtggatg ctcaccgctg gaaccggacc gcgcagcaga tccccgatcc tggcgtcgtg
     1261 ctccatctgc ggagccgccg tgaggatgcc gtcgacacga cgctcgagca gcatacgcag
     1321 gtagcgcgcc gcgtccgtcc cctcctggtc gatgctaccg atcaagaccc cgtggccctg
     1381 gcggcgtgcc tcgcgctcgg cgccgacgac gaaacgcgca agctcggcat cgctcaggtc
     1441 gctggccact atgcctatcg tgcacgtgct ccgggtcacg aggctcctgg ccagggcgtt
     1501 cgggacgtat ccgagggcat tcgcggcctc gaggatgcgc tccctcgtct ccggggagac
     1561 ggttcccttg ccgttcagca ccttgccagc cgtctggaag ctcacgccag ccttcgaggc
     1621 aacgtcccgc aacgatacgt gcgtcagagg accactcctt tcaacaccag cccgtacgct
     1681 ggactactca gactagctct tcaggcctgt gttcgcgatg ccctgcacga tctggcgctg
     1741 gaagaagagg aagacgatga tcagcg
//
LOCUS       DATAIR010000733         7900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_262195, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000733 DATAIR010000000
VERSION     DATAIR010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262195"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHM69_17395"
     CDS             <1..215
                     /locus_tag="VHM69_17395"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX2742218.1"
                     /translation="IEAASLQYVRKVTGFNKPSQTNEAAFYSAVEDIARISERLLASL
                     QTTASPKDREEEAAKAKARSALRFGG"
     gene            522..2018
                     /locus_tag="VHM69_17400"
     CDS             522..2018
                     /locus_tag="VHM69_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2742219.1"
                     /translation="MRKIGVVLAAVALAVGLLFFGSYTGATEEMSKAQAQTVTTATVQ
                     PNIIFILTDDMRKDDLKYMPKTKALLQDTGITFQNAFVSNALCCPSRATIMRGQYSHN
                     NGVWSNSSTDSSSTTSGGWQAYQDNGNEADNVATRLHDAGYRTGLFGKYLNRYTDTIH
                     KPQGWDRWFAAFSSGDPRYLDYDVNDDGTIMHFGTSDSDYSTDVLSSQTNAFISASAS
                     QGSPFFAYVAPLAPHLPSTPAPRHVHAYDGVKAPRLPSFNESNVSDKPSWIRQLPSLT
                     TAEISAMNKRHEKRIESLQAVDDLVEGVVNTLNNANVLSNTYIFFTTDNGFHHGEHRV
                     PSQKWRPYEEDVHVPLLVRGPGVAAGSTTYKLALNTDYLPTFTDLACSSSPCSTQNWS
                     YVPDGRSLAPVLDGSVTNWRSAVLLEAAANYSPAYYGIRTISTNGIPKRKYVEYAGSE
                     RELYDLDPDPYELANYYSPSPDVDVLAARLQALKSCAGDTCFTAENGP"
     gene            complement(2273..3004)
                     /locus_tag="VHM69_17405"
     CDS             complement(2273..3004)
                     /locus_tag="VHM69_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX2742220.1"
                     /translation="MVVTFLIGCAFLLLVVGCAGTSSESSNKKEHGSSPQATESEEEA
                     RCEGTRSYHIYQVIHKPGYWNGPLRTGSQEDIKEADKKAGQETMDFGVYTTNDLPGCL
                     KGGLLLGTEKPDMLDGKHGDDEVRGLGGSDHYLDGGDGNDIIYGGDGDDDPNNKGGEG
                     LVGGDGNDTLYGGDGDDSVHGNNGEDGLYGGDGNDIVTDWYDEQRDKLYCGEGRDEYV
                     AGKNDYVGSSCEKNVSPQRIFGGEA"
     gene            complement(3352..3621)
                     /locus_tag="VHM69_17410"
     CDS             complement(3352..3621)
                     /locus_tag="VHM69_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX2742221.1"
                     /translation="MRRLLRRECTHLDQINNVEPSAQGCEECLRMGDTWVHLRECLTC
                     GHVGCCDQSNNKHATEHFHHTGHPIIKSFEPGEDWRWCYLDKMLV"
     gene            complement(3633..4559)
                     /locus_tag="VHM69_17415"
     CDS             complement(3633..4559)
                     /locus_tag="VHM69_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742222.1"
                     /translation="MGGVSGVTTALGPILGGFLVENVSWRAAFLVNVPLAVAVLLIAW
                     RHVPESRDPNARKLDIPGVVLATTGLGGALYFLPLLLIQVHGYSATAAGSSFLPFVII
                     TFLMSRWAGGLITRFGARPPLVIGPLIAAAGFVLFAIPGTGGSYWTTFFPAIVVLGFG
                     MALVIVPLTTTALNSVSGRHSGLASGVNNAVARTAGLLAIPVLGIFVFHIFSGAFDAR
                     AQSLDLSPQVQKQLESEKEDLGGAESPSGVSGEKAAQIERAIDESFLAAFRLAMLIAA
                     GLAVASAVAAAVIIEGKERAAPAERVGQTAPA"
     gene            complement(4466..5032)
                     /locus_tag="VHM69_17420"
     CDS             complement(4466..5032)
                     /locus_tag="VHM69_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742223.1"
                     /translation="MSNIGNPPCNVGVMRSRKADTPCAPRSGPWILAATIIGSAMAFI
                     DSTVTSVALPQIQERLGATAVDAQWIVESYALFLSALILVGGSLGDHYGHKRDFSLGV
                     LTFTLASVWCGLSTSPEMLILARAVQGIGGAMLVPGSLSIISASFAGEQRGKAIGTWA
                     GSRGSRPPWGQFSAGSWSRTSRGGLHSS"
     gene            complement(5046..5624)
                     /locus_tag="VHM69_17425"
     CDS             complement(5046..5624)
                     /locus_tag="VHM69_17425"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX2742224.1"
                     /translation="MPSRSDEVLDFWFGREDEPGYGEFRAEWFSKDPAFDAEVTAQFG
                     ELYEEAASGDLEGWRDDARSCLALVIVLDQFPRNMFRGDGRTHATDSAALETSKYAVE
                     HALDRELPGFQRMFLYMPFMHCEYVEDQRRSVELFRRLAEEPDAPDVTSYAAGHREIV
                     ERFGRFPHRNEILGRQTTPEEAEFLKTEGSSF"
     gene            complement(5614..6201)
                     /locus_tag="VHM69_17430"
     CDS             complement(5614..6201)
                     /locus_tag="VHM69_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX2742225.1"
                     /translation="MGQSQLFAEICSAKRKVNTEVLKQTVSLAVEIAREGREGRKIGT
                     LFVVGDSGAVIKRSQPLILDPLQGHPDESKQIDNPDMRETIKELAQLDGAFLVSNSGV
                     VISAARYIDAASDNLDLPLGLGSRHMAGASISRQTSAVAVVVSESSMVRMFDDGELVS
                     EIIPELWMIDGYTSRMDGRIFTRRDEDITVIGRAE"
     gene            complement(6204..7616)
                     /locus_tag="VHM69_17435"
     CDS             complement(6204..7616)
                     /locus_tag="VHM69_17435"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742226.1"
                     /translation="MNHVRNTQDDNKVLARRFLEAQAKGDIETLDDLMAPDFADRSLL
                     PGQKPGREDYKQSVADMLAVYSNTGFTVEAQIAEGDHVVSRYTGSSIHRGKFLGVDPT
                     GRESGYSGIAIHRIAGGKIAEEWAEADNLEVVQSALELELRRRERIEQEKMAALGKLS
                     AGLAHELNNPAAAARRAAEELREAILKAQLLALENDGRLSNEVREVLVGMLHEATANV
                     VLDPLSQSDREQELTDWLEEKGFREAWDLAPILGAAGLSAERLEELASEMGDSETLTG
                     SLEWLGATLQLTDLADEVGQSAGRISDLVRAMKEYTYMDRAAYAGTDVREGLETTLTI
                     LGHKMKGVALQREYEENLPEIWANAGELNQVWTNLIDNAIDAVDKHGKIQVRTAREND
                     QVLVEVVDDGPGIPEEFRSRIFDPFFTTKEIGEGTGLGLDIVRRIVAGHGGELTFHSE
                     PGETRFTVRLPVNTIQNGGS"
     gene            complement(7613..>7900)
                     /locus_tag="VHM69_17440"
     CDS             complement(7613..>7900)
                     /locus_tag="VHM69_17440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742227.1"
                     /translation="GSGHIRLRTIFEGERVLGEISDDGPGIPEEIQERIFEPFFTTKD
                     VGKGAGLGLDVSYRIVVGRHRGDIRIVSKPGHTRCEVRLPMKTPLEQERED"
BASE COUNT         1512 a         2574 c         2232 g         1582 t
ORIGIN      
        1 agatagaggc cgcttcgcta cagtacgtga ggaaggtaac cgggttcaac aagccgtccc
       61 agacaaacga agcagccttc tactccgcag tcgaagacat cgcgaggatc tcggagcggt
      121 tgctcgcctc gctccaaacc actgcatctc caaaggaccg ggaagaagag gcggccaagg
      181 cgaaagcgcg ctcagcccta cgattcggcg ggtgatcaaa gctgggatct actccgcgca
      241 actgagaatg agtgctgttc gaagttcgcc tggggctgat agtcggcagt tcgatagccc
      301 cggtccaaca ccgaagcgcc cctgacctcc ttggaatagc cacctggcca agaccccgat
      361 ccgcggaaat ttgcaagaag tttcttcaaa agtagtcctg cgcaccgctc agattcatca
      421 gagattcatc gccacccgct acgctacctt cgttaaaggt gtcgtggggc ggggcctggc
      481 accctgcttt cctccgccca actaaggaaa ggttacaagg gatgaggaag atcggtgtag
      541 tattggctgc ggttgcccta gctgtagggc tgctcttctt tggctcttac actggcgcca
      601 ccgaagaaat gagcaaagcg caagcccaga cggttacaac ggctaccgtc caacccaaca
      661 tcattttcat cctcaccgac gacatgcgca aggacgacct gaagtacatg ccgaagacca
      721 aagcgctgtt gcaggatacg ggcataactt tccagaacgc tttcgtctcc aacgcgctct
      781 gctgcccttc gagagccacc atcatgcgcg gtcagtactc ccacaacaac ggggtctggt
      841 ccaactcctc cactgacagc tcctcaacta ccagcggcgg ctggcaagcg taccaggaca
      901 acggtaatga ggcagacaac gtagccacgc gtttgcacga cgctggctat aggaccgggc
      961 tctttggcaa gtacctgaac cgctacactg acaccatcca caagccacag ggatgggata
     1021 gatggttcgc cgctttctct tccggcgacc cccgctacct cgactacgac gtcaacgacg
     1081 acggcaccat aatgcacttc ggaactagcg atagcgatta ctcaaccgac gtgctcagta
     1141 gtcagaccaa cgcgttcatc agtgccagtg cctcgcaagg tagtcccttc tttgcctacg
     1201 tggctcccct tgccccgcac ctgccgtcca ctccagcccc gcgccacgta cacgcctacg
     1261 acggcgtgaa ggctccgcgc ttgccctcct tcaacgagtc gaacgtctca gacaaacctt
     1321 cctggatcag gcagctaccc agtctcacaa ccgccgaaat ctctgccatg aacaaacgcc
     1381 acgaaaaacg gatcgagtcc ttacaggcgg tcgacgattt ggtggagggg gtggtcaata
     1441 cgctcaataa tgccaatgtg ttgagcaaca cttatatctt cttcactacc gataacggct
     1501 tccaccatgg cgaacaccgc gtaccctcgc aaaagtggcg cccctacgag gaggacgtac
     1561 acgtgccgct cctggtgcgc ggccccggag tggcggcggg ttccaccact tacaaactgg
     1621 ccctcaacac tgactacctg cccaccttca ccgatcttgc gtgctcttcg agcccgtgta
     1681 gcacccagaa ctggagttat gtccctgacg ggcgctcact tgcacccgtg ctcgatggaa
     1741 gcgtaaccaa ctggaggagc gccgtcctgc tcgaagccgc ggccaactac tcaccagcct
     1801 actacgggat ccgcacgatc agcaccaacg gcatccctaa gcgcaagtac gtcgagtatg
     1861 caggcagtga aagggagctc tacgacctgg accccgatcc ctacgaactc gctaactact
     1921 acagcccatc acccgatgtc gatgtgcttg cagcgcgtct gcaagcacta aagagctgcg
     1981 ccggagacac ttgcttcacg gctgagaacg gaccgtagta gttctccagt agccacaaga
     2041 gggccaagag gggacgggcc ttccgaactc cgccccctag gctctcattc acccgaatgc
     2101 gtggaaggaa gatactgcga acttcgccat gacggcgttc tcggctcctc tttgtcaagc
     2161 tccccgcata tccccggtcc tatgccgata atacccgccg gacatatcat ggcccctctc
     2221 gcgcaggccc tcttgaccga ggctatgggc tattcaccga tgtcctaggg aatcaagcct
     2281 cacctccgaa gatacgctgg ggcgagacgt tcttctcgca actgctgccc acatagtcgt
     2341 tcttgccagc gacgtattcg tcccgacctt caccgcaata gagcttgtcc cgctgctcat
     2401 cataccagtc agtaacaata tcgttgccat ccccgccata gaggccatcc tctccgttat
     2461 ttccgtgcac actgtcgtcg ccgtcgcctc cgtagagggt atcgttaccg tctcctccaa
     2521 cgagaccctc gccgccttta ttattgggat cgtcgtcgcc gtcgcctccg tagatgatat
     2581 cgttaccgtc tcctccatcg agatagtgat cggagccgcc aaggccgcgt acctcgtcat
     2641 cgccatgctt cccgtccagc atgtcaggtt tttcggtgcc taagagcaga ccacccttga
     2701 ggcagcccgg taggtcgttg gtggtataga ccccaaagtc catcgtctct tgaccagcct
     2761 tcttatcagc ctcctttatg tcttcctggc taccagttct caacggtcca ttccaatagc
     2821 cgggtttatg gatgacctgg tagatgtggt agctcctcgt cccctcgcat cgggcttctt
     2881 cttcggactc tgtagcttgg ggtgagcttc cgtgctcctt cttgttggag gattccgagc
     2941 tcgtccccgc acagccaacc actagcagaa gaaaggcgca cccgatcagg aaggtcacca
     3001 ccacagctat aaggtgtcct tggcgaacca cggcgcaccc gccttccctt acatgtgcct
     3061 tgctactagc acctccaagc atgccacata acgaaccctg gcgcatctct acgcaggctt
     3121 attcagcgga atgcgtagaa gggtaattct gcgaaacccg gatgcagcat cgtgcatgat
     3181 tctgtcgagg aaggcgagaa gagtgactac agagcccgtg aggacatatg cccgagcgca
     3241 tcatgtggac agcatgcata gacattcacc ctgatgaaag ctgcacgtcc ggcgtaaacg
     3301 ctactcgtcc tttgccactt cgcgagcttt gtgtagatca atcctgctgt gtcagacgag
     3361 catcttgtcc aggtaacacc atcgccagtc ttctccgggc tcgaacgact tgatgatcgg
     3421 gtgtcccgtg tggtggaagt gttcggtagc gtgcttatta ttggattggt cgcaacaacc
     3481 cacgtggccg caggtcaggc actcgcgcag gtggacccaa gtatcgccca tccggaggca
     3541 ctcctcgcat ccctgggcgc tcggctcgac gttgttgatc tgatctagat gcgtacactc
     3601 tcgtcgcagc aatctcctca taggtgttct cctcacgcgg gggcagtttg gccgactctc
     3661 tcggcgggcg cggcgcgttc cttgccctct attatgaccg cggcggcgac ggcgcttgcg
     3721 acggcgaggc ccgcggcgat caacatcgca agccggaagg cggcaaggaa cgactcgtcg
     3781 atggcccttt cgatctgggc cgccttctct ccgctgacgc ctgaggggct ctccgctccg
     3841 ccgaggtcct ccttctcgga ctcgagctgc ttctgtacct gcggcgagag atccagactc
     3901 tgcgcgcggg cgtcgaaggc gccggaaaag atgtggaaga cgaagatgcc gagcacgggg
     3961 atcgcgagca ggcccgccgt cctggcgacg gcgttgttta cgcctgaggc caggcccgag
     4021 tgcctgcccg agaccgagtt cagagccgtg gtcgttagcg gcactataac taacgccatc
     4081 ccgaagccga gcacgacgat cgcagggaag aacgtagtcc agtaggagcc tcctgttccg
     4141 ggaatggcga aaagcacgaa ccccgcggcg gctattagcg ggccgatgac cagcggcggc
     4201 cgcgccccga agcgggtgat caaaccgcct gcccaccgcg acatcaggaa ggtgatgatg
     4261 acgaaaggca ggaacgaact acccgccgcc gtcgctgagt agccgtggac ctggatcagc
     4321 aggagaggca agaagtacaa cgccccgccg agccccgtcg tcgccaacac cacgcccggg
     4381 atgtccagct tcctggcgtt gggatcgcgg ctctcgggga cgtggcgcca ggcgatgagc
     4441 agcacagcga ccgcgagcgg cacgttcacg aggaatgcag cccgccacga gacgttctcg
     4501 accaggaacc cgccgagaat tggccccagg gcggtcgtga cccccgagac cccgcccacg
     4561 tgcctatggc cttcccgcgt tgctcgcctg cgaaggaggc gctgatgatg gatagtgacc
     4621 ctggcacgag catcgcgcca cctatgccct gcactgcgcg ggccaggatc agcatctccg
     4681 ggctcgtgga gagcccgcac cataccgagg cgagagtgaa ggtcaatacg ccaagtgaga
     4741 agtcgcgctt gtgcccgtag tggtccccga gggagccgcc gaccaggatc agggccgaca
     4801 agaacagggc gtaggattcg acgatccact gcgcgtcgac ggcggtcgcc ccaagccgct
     4861 cctgtatctg cggaagggcg acgctcgtca ccgtgctgtc gatgaaagcc atcgccgacc
     4921 cgatgatggt cgcagcaagg atccagggcc ccgaccgcgg cgcgcaaggc gtgtcagcct
     4981 tcctggagcg catgaccccg acgttacaag gaggattgcc tatattcgac acgccggcga
     5041 tcctctcaga acgatgaacc ttccgtcttc aggaactcag cctcctccgg ggtcgtctgg
     5101 cggcccagga tctcgttcct gtgcgggaac cgcccgaacc gctcgactat ctccctgtgc
     5161 ccggcggcgt atgaggttac gtcgggcgcg tcgggctctt ccgcgagccg ccggaacagc
     5221 tccacggagc ggcgctggtc ctctacgtac tcgcagtgca taaacggcat gtacaggaac
     5281 atcctctgga aaccaggcag ctccctgtcg agcgcgtgtt cgacggcata cttggaggtt
     5341 tcgagtgccg ctgagtccgt cgcgtgggtg cgtccgtcgc cgcggaacat gttgcgaggg
     5401 aactggtcga gcacgatgac gagcgccagg caactcctgg catcgtcgcg ccagccttcg
     5461 aggtcgccag aggcggcctc ctcgtagagt tccccgaatt gcgctgtgac ctcagcgtcg
     5521 aaggcggggt ctttgctgaa ccactcagcg cggaactcgc cgtagccagg ctcgtcctcg
     5581 cggccgaacc agaagtcgag gacctcgtcg gacctactcg gcacggccga tcaccgtgat
     5641 gtcttcgtcg cgacgggtga agatgcgtcc gtccatcctg ctcgtgtaac cgtctatcat
     5701 ccaaagctct gggattattt cggagaccag ctcgccatcg tcgaacatcc tgaccatcga
     5761 gctctcggag accacaacgg ccacggcgct cgtctgcctg gagatcgatg cgccggccat
     5821 gtgacggctg ccgagtccga gcggtaggtc gaggttgtcc gaggccgcgt cgatgtagcg
     5881 ggccgcggag atcaccacgc cggagttcga gaccaagaac gcgccgtcga gctgggccag
     5941 ctcctttatg gtctcgcgca tgtcaggatt atctatctgt ttgctctcgt cggggtgtcc
     6001 ctgcaagggg tcgaggatca ggggctgcga ccgtttgatg accgcgccag agtcaccgac
     6061 gacgaagagc gtcccgatct tgcgcccctc gcggccctcg cgcgcgatct cgaccgcgag
     6121 cgagacggtc tgcttgagca cctccgtatt caccttccgc ttcgcggagc agatctccgc
     6181 gaacaactgc gactgtccca ttactatgac cctccgttct gtatagtatt taccggcaac
     6241 ctcaccgtga agcgcgtctc tcccggctcc gagtggaatg tgagctcccc gccgtggccg
     6301 gcgacgatgc gcctgacgat gtccagcccc agtcctgtcc cttcaccgat ttccttggtc
     6361 gtgaagaacg ggtcgaagat tcgactccgg aactcctccg gaatgcccgg accgtcgtcg
     6421 acgacctcga cgagcacttg atcgttctca cgggcagtgc gtacctggat tttaccgtgc
     6481 ttgtctacgg cgtcgatggc gttgtctatg aggttggtcc acacctggtt aagttcgccg
     6541 gcgttggccc atatctccgg tagattttct tcgtattctc gctgtagggc cacacccttc
     6601 attttatgcc caaggatcgt gagcgtggtc tccaggccct ctcgcacgtc ggtcccggcg
     6661 tacgcagccc tgtccatgta cgtgtactct ttcatggccc tgaccagatc ggagatcctg
     6721 cccgcgctct ggcccacctc gtccgcaaga tctgtcagct gtagagtcgc tccgagccac
     6781 tcaagggaac cggtgagagt ctcgctgtca cccatctcgg aggccagctc ttccaggcgc
     6841 tccgcagaca ggcccgcagc gcccaggatc ggcgcgagat cccaggcttc tcggaagcct
     6901 ttttcctcaa gccagtctgt gagttcctgc tcccggtcgc tctgggagag tggatcgagg
     6961 acgacattgg ccgtggcctc gtgcagcatc ccaaccagca cctctcgtac ttcgttggag
     7021 aggcgtccgt cgttttccag ggcgaggagt tgcgctttga ggatcgcctc gcgcagttcc
     7081 tcagccgcgc gccgcgccgc cgcggccggg ttgttcaact cgtgggcgag cccggcggac
     7141 agctttccca gtgccgccat cttctcctgc tcgatgcgct cgcgtctgcg cagctccagc
     7201 tcgagggcag actgcaccac ctccaggttg tccgcttccg cccactcctc cgcgatcttg
     7261 cctccggcta tgcggtggat ggcgatgccg gaatagcctg actcccggcc cgttggatcg
     7321 acgccaagga acttgcccct gtgaatgctg cttcccgtat acctgctgac cacgtggtca
     7381 ccctcggcga tctgagcctc gacggtgaag cctgtgttgg agtacacggc gagcatgtct
     7441 gcgaccgact gcttgtagtc ctcgcggccc ggcttctggc ccggcaggag gctgcggtcg
     7501 gcgaagtcgg gagccatcag atcgtcgagc gtctctatat ccccttttgc ctgcgcctcc
     7561 aggaaacgac gagccaggac cttattgtca tcctgggtgt tccttacgtg gttcaatctt
     7621 ctctttcctg ttccaaaggc gttttcatgg gtaaccggac ctcgcagcgg gtgtgaccgg
     7681 gctttgagac gatgcggatg tccccgcggt ggcggccgac gacgatccgg tagctcacgt
     7741 ccagtccgag gccggcgccc tttcccacgt ccttggtggt aaagaaaggc tcgaagatgc
     7801 gctcctggat ctcctctggt atgcccggcc cgtcgtcgct gatctctccg agcacccttt
     7861 ctccttcgaa gatcgtcctg agccttatgt ggccgctccc
//
LOCUS       DATAIR010000734        15203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_216617, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000734 DATAIR010000000
VERSION     DATAIR010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216617"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VHM69_17445"
     CDS             <1..194
                     /locus_tag="VHM69_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742228.1"
                     /translation="SSPLLRRRVPPSAPDGVLHAEVVEDPGDEEVDDSGLYATGTKAG
                     PTATPSEAPMPISFTDWLN"
     gene            complement(191..448)
                     /locus_tag="VHM69_17450"
     CDS             complement(191..448)
                     /locus_tag="VHM69_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX2742229.1"
                     /translation="MAEREATVAAEEGIHGRPAAQLVKAAKRFSSQIVLVKGNREVNA
                     KSVLKITGLAKKGEKITIRAEGEDAEQAVEALADLISADGH"
     gene            complement(546..1307)
                     /locus_tag="VHM69_17455"
     CDS             complement(546..1307)
                     /locus_tag="VHM69_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX2742230.1"
                     /translation="MRENRLRTIWREGGSVVNGWLHVPSSFSAEVMAHAGWDSLTIDM
                     QHGPVDHGSLVPMLQAISTTDTVPVVRVAWNDPGPIMQVLDAGSYAVICPMINTREEA
                     ETFVGACRYPPQGYRSYGPYRATLYAGSDYTDHANETVVTMAMIETREALENLEEILG
                     VPGLDAVFIGPSDLGQSLGHGPGTDRDETAVVEAIDRVLAATKKHGLVAGIFTGSPEY
                     ASRMVEKGFQFVTVSSDARLMAAAAAGVISAVKGE"
     gene            1487..2083
                     /locus_tag="VHM69_17460"
     CDS             1487..2083
                     /locus_tag="VHM69_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742231.1"
                     /translation="MPEGTSSQGQMCDEELARSDCEVAVHMTRRLGLGTGVQCGLNEI
                     YERWDSKGNPRKLSGEDIALPARFAQVASQEVLFECIGGPDLASEMISRRAGAALDPS
                     IAETFLKYSRELLAEIITADAAVAVIGAGPHRRIAEPRLDEVARAFAELVDLKSPFMH
                     GHSSLTIPRTKQVRRRRGLEVTPARQRSIVANLSPKNA"
     gene            complement(2041..2388)
                     /locus_tag="VHM69_17465"
     CDS             complement(2041..2388)
                     /locus_tag="VHM69_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742232.1"
                     /translation="MRAEQTVSEMVVESLARRAEALSEGSGLTFEDAFAEVLKTPAGR
                     QLAELADGPHRHEKAAEWQKGLLAERDARRPAHLRESGSVQSRYTWLGRYEEDTYEEY
                     YAFLGERFATIER"
     gene            complement(2709..2849)
                     /locus_tag="VHM69_17470"
     CDS             complement(2709..2849)
                     /locus_tag="VHM69_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742233.1"
                     /translation="MGRYGGRKYVAVEVRLADGRYRLFWPEDLKAASTRLPRCRPLIV
                     GR"
     gene            3351..4562
                     /locus_tag="VHM69_17475"
     CDS             3351..4562
                     /locus_tag="VHM69_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742234.1"
                     /translation="MKYKGAIIQQPVPDEEFHQGQQGESALQNGEGHFHRLLEKLPAG
                     AYTCDPEGLITYYNQHAVQVWGRAPKLNDPAYRFCGSFKLYATDGTPITHDRCWMALA
                     LKENEGYNEREIVVERPDGRRLTALAHANPIHDESGNLIGAVNVLVDITERKRTEEEL
                     RIVREAERSRIARDLHDGVLQDLAAVVQRLEATCIEQEAYSDGRAHSIDLHHEIDTLR
                     HTALKLRQAVYYLRSEKAPSILRAVEALVEVNLQRAPECDVRLEVQDDFPHEVPGEAG
                     REMLRIVQEALTNARRHSRARNVRVSLKAEGTDSLVAEVSDDGRGFDLTSVKGGLGLE
                     GMRERTTLLGGDLTVYSEPGKGTSVRVRLPGLQVSHEDQAPVSEEREARGYASRTLAV
                     LGSQDHGGRRI"
     gene            complement(4507..5178)
                     /locus_tag="VHM69_17480"
     CDS             complement(4507..5178)
                     /locus_tag="VHM69_17480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742235.1"
                     /translation="MSEEGVIRVAVVDDQRLFVDGLVRILGTQPEMDVVGRAHTGEEA
                     VELCLSKEPDVVLMDLSMPGMGGINATRQILGLLPRTQILVLTVHTDDTNLFQAIKAG
                     AQGYILKDCTPEDLTSAIKSVHAGNTITSPDIAKKLITTFEGIRSNTALTPNLTDREL
                     EVIKALAQGKSNKVIARDLGISEKTVRNHASNIYKKLHIFDRTQAVIYAVRRGLVNLE
                     QLESD"
     gene            5340..6125
                     /locus_tag="VHM69_17485"
     CDS             5340..6125
                     /locus_tag="VHM69_17485"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX2742236.1"
                     /translation="MPCQDDPLGADDPDGSGLAALTNNPGQGEVCFKLGVEDIILPAI
                     GAHSYSAHPGANGPIVVPLTPPDENRVSGGCAEVSDNVVVKIIHNHEGFVVNTTDFAS
                     GAIRGQLPNGRSRVRSSAGVRVEDLGDERTYPLRSQTAEFEHFEGSLAILSGTYVLGL
                     YSPASVSVWFGKGKALGASVRFWPQVLSYKEAIFLESHEARIGTLVRVLDGARRAGKG
                     RFGTIETTYGHPTYLALDVRFEDGSVELYWYHELERSKVSSLA"
     gene            6207..6413
                     /locus_tag="VHM69_17490"
     CDS             6207..6413
                     /locus_tag="VHM69_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742237.1"
                     /translation="MEAYEARRGTVVRVRDGHWKSQFAGMYGTVQACWGQSEHAAVDV
                     LLEDGRSELFWPPNLETIDRDLAV"
     gene            complement(6946..8310)
                     /locus_tag="VHM69_17495"
     CDS             complement(6946..8310)
                     /locus_tag="VHM69_17495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2742238.1"
                     /translation="MSELMPGLTGHAKKVILHVDDNPTTRYTIGRTLEREGFKVSQAV
                     SGREGLRLAREADLIILDVKLPDIDGFEVCRRIKADPKTADIPVLHLSATYVTYEAQA
                     TGLEGGADGYLVQPVDERVLVATIKAMLRMRRAEEELHRIFTLSPDLLCVVGSDGYFK
                     RVNPAFEDTLGYASRELLARPFVDFVHPEDREAAVEEVAKLSTDFPTSHFENRFRRSD
                     GSYVWLEWKAVPVAKEGLIYAAARDVTESKQLHETLLEIREAERRRIARDLHDVVLQD
                     LAAAVQGLQANQAETKDSARGIDLHHEINALRKAVSSLRSAIYDLRLEGSQPFVRAVE
                     ALVEFNRQLAPEREITLAVQDGLPPELPHVAHVEMLRVLREALVNVRRHSDAQHVMVA
                     LRKNRDMMQVSVTDDGRGFDRASVREGVGLSAMRERAIWAGGRLQIDSTPGNGTRVSV
                     AVSL"
     gene            complement(8324..10129)
                     /locus_tag="VHM69_17500"
     CDS             complement(8324..10129)
                     /locus_tag="VHM69_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742239.1"
                     /translation="MTLPILTVAIHSEDDVVTARQRARQTAGLLGFDRQDQTRVATAV
                     SEIARNAFAYAGGGRVEFRVDEKSDPPLFLTCVIDHGPGIENVRQILAGQYRSSTGMG
                     LGIIGAKRLMDLFEIESSPARGTTVLMGKHIPRGTATFARANVAAISNELAQLAPRTP
                     SAELQQQNQELLRTLDELHKRQEELTHLNSELEDTNRGVVALYAELDEKAEQLQRANE
                     LKTRFLSNMGHEFRTPVNSILALTRLLLERTDGVLTEEQEKQIAFIRQAAQDLSDLVN
                     DLLDLAKVEAGMIAVRPEEFLVADLFATLRGLFRPLVVPEVTLHFEEPNGIPPLHTDE
                     GKVSQILRNFVSNALKFTERGHVRVSATLDAAREQVVFAVADTGIGIASEDQGQIFEE
                     FAQAEHPIQRLTKGTGLGLPLARRLTELLGGSVSLQSAPGRGSVFSAVVPLVYSGPAQ
                     VPAEPERHRLQTPAPVVNTEHSDSILIVDDDEAARYVLKRYLAETSFTIIEATNGPEG
                     LRMARENRPQVVLLDLMMPEMSGFEVLQRLKSDPVTRDIPVIIITSKPLQDRDRSSLD
                     EQTIAILSKENLSLEAAIARVREAITHAGVASDQP"
     gene            complement(10126..11112)
                     /locus_tag="VHM69_17505"
     CDS             complement(10126..11112)
                     /locus_tag="VHM69_17505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEX2742240.1"
                     /translation="MSQVAETRRAASGLASKLAFDEAGIGKVALIVTEAATNLAKHAI
                     EGEILLYAIQSGRIGGIQVLALDRGPGLANAAQALRDGYSTSGSPGTGLGAIERLATL
                     FDIHSVPGVGTALLARLWSKPMPATRQPRLEVGALSLPKPGEDVCGDRWALARFPERT
                     MILVADGLGHGPGAAEASLEAERVFHERAALAPAAIVESIHAALRSTRGAAIAVTEIA
                     PSHETVRFAGVGNISGFVLSGEGSRQMVSHNGTAGHSARRIQEFTYPWFSDALLVLYS
                     DGLISHLDPRRYPGLLNHHPDIIAATLYRDYTRGRDDVTVVAAREAKSGTTG"
     gene            complement(11169..11573)
                     /locus_tag="VHM69_17510"
     CDS             complement(11169..11573)
                     /locus_tag="VHM69_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX2742241.1"
                     /translation="MVITKDEIVPVRSSEDIVRVRQAVRNWSIDLGFGLVDQTKLVTA
                     ASELARNTVNHGGGGTVRMGVVSNAAHFGLQLTFEDHGPGIADVELALRDGYTTGGGL
                     GLGLGGSRRLANEFEIESHPGEGTRVRIVRWT"
     gene            complement(11554..11949)
                     /locus_tag="VHM69_17515"
     CDS             complement(11554..11949)
                     /locus_tag="VHM69_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2742242.1"
                     /translation="MDRIPILKMGAFLLVTIQVDMHDRLAIALQNDLMDRIASTSAQG
                     VLIDISALEIVDSFIGRMIGNIAAMSRVLDAETVVVGMRPAVAITLVELGLSLSGVRT
                     ALNVEKGMNLLQASLPLTAEESADGNNEG"
     gene            complement(11954..12814)
                     /locus_tag="VHM69_17520"
     CDS             complement(11954..12814)
                     /locus_tag="VHM69_17520"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2742243.1"
                     /translation="MIENSMIPSILKDHEEELLEDWVQEQLAADTRRADLISEFELSQ
                     QSAEFLRAFRQALQDGNVNKFENPHWDHVREILTDLSRSRAQQGFSALETATFVFSLK
                     KPLFTRLRRHLENHADTLVEENWAATVLLDRLGLFTTEVHQQNREAVIARQQQELLEL
                     STPVVKLWEGILALPLIGTLDSARTQVVMESLLQQIVESEATIAIIDITGVPTVDTLV
                     AQHLLKTVAAARLMGADCIISGIRPQIAQTIVQLGVELNEVTTKASMAAAFRLALQRS
                     GQSLAQTARR"
     gene            complement(13056..13283)
                     /locus_tag="VHM69_17525"
     CDS             complement(13056..13283)
                     /locus_tag="VHM69_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742244.1"
                     /translation="MHSTLQTWATEVVPEARATVISLFAAALFSGSGLATMAAAPLAE
                     AGAFDLLIALVAVPLGLFAGLARHGYSGNRQ"
     gene            complement(13639..14388)
                     /locus_tag="VHM69_17530"
     CDS             complement(13639..14388)
                     /locus_tag="VHM69_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742245.1"
                     /translation="MRNDGAMLGALLLFAIELTILYAAGSYAMGRLMRMSGFGGGGSV
                     LGRIAFYLLMFPGVVLHECSHYLACLLTGTRVTRFAPFSPGRSNDGRLTLGYVRHERR
                     ALPVGAIIGLAPILLNPLGLLFVTALLTPLTLHEVAKPSVMIALEGIFASGFLTDTPL
                     LATSWAYLSLSFALASVPSREDLSSLPALLLVFGGGILLAGLLRAGSERVLASAIDDL
                     SVLAAGLYALPATVAVFAAVAIGLSGRAIRG"
     gene            14514..>15203
                     /locus_tag="VHM69_17535"
     CDS             14514..>15203
                     /locus_tag="VHM69_17535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2742246.1"
                     /translation="MKLGISTLVTDEGIAPGALAGAIEERGFDSLFVAEHTHIPLSRK
                     SPWPRGGELPRRYYRTLDPFVTLTAAAAATERLLLGTGVALIIERDPIITAKEVASLD
                     LVSGGRVVLGVGVGWNREEMENHGTDPRTRGKLMDERIRAMIEMWTRDEPEYHGQFVD
                     FDPIGVWPKPVQKPYPPIYVGGGSRAFERVARFGDAWLANGLPPDKLAPMMSELREVA
                     GRDVPVSVFNAS"
BASE COUNT         2972 a         4657 c         4545 g         3029 t
ORIGIN      
        1 catcgtcacc cctcctccgc cggcgagtcc ccccatcggc gcccgacggc gtgctccacg
       61 ccgaggtggt cgaggacccg ggcgacgagg aagtcgacga ctcgggcttg tacgcgacag
      121 gcacgaaggc cggtcccacc gccactcctt ccgaggcccc tatgccgatc agcttcacgg
      181 actggctcaa ctagtgtccg tcggcggaga tgagatcagc cagggcttcg acggcctgct
      241 cggcgtcttc accctctgca cggatggtta tcttctcgcc ctttttggcc agccccgtta
      301 tcttcaacac gctcttggcg ttgacctccc gattgccctt tacaagcacg atctgcgaag
      361 agaacctctt agcagccttc accaactgcg ccgccggccg accgtggatt ccctcctcag
      421 ccgcgaccgt cgcttctctc tcagccatga gtcgctcctc tcaccataag atccaggacg
      481 cggcacccat cataccccac ccgaaccggg atgaagttcc ggcacgacag ggccactctt
      541 ttcacttact cgcccttgac ggccgagatg acgccggccg cagccgcggc catgagccgc
      601 gcatccgacg aaacggtgac gaactggaaa cccttctcga ccatgcgcga ggcgtactcg
      661 ggcgagccgg tgaagatccc tgcgacgaga ccgtgttttt ttgtggcggc cagcaccctg
      721 tcgatagcct cgaccacagc ggtctcgtcc ctgtccgtgc ctggtccgtg cccgaggctc
      781 tgacccaggt cagatggacc gatgaagacc gcgtcgagtc caggtacgcc gagtatctcc
      841 tccaggttct ccagggcttc ccgagtctcg atcatggcca tcgtcaccac ggtctcgttt
      901 gcgtgatcgg tgtagtcgga gcctgcgtag agggtcgctc tgtacgggcc gtagctacgg
      961 tatccctgcg gcggataccg gcaggctccg acgaaggttt cggcttcctc gcgggtgttt
     1021 atcatcgggc agatcacggc gtagctcccc gcgtcgagca cctgcattat ggggccggga
     1081 tcattccacg ctacccgcac tacaggtacg gtatctgtcg tggagatggc ctggagcatc
     1141 ggaacgaggc tcccgtggtc caccggcccg tgctgcatat ctattgtcag gctgtcccat
     1201 ccggcgtgcg ccatgacctc ggcggagaaa gagcttggaa cgtgcaacca cccgttcacg
     1261 acgcttccac cctcccgcca gatggtcctc agacggttct ccctcaccac ttatcccttc
     1321 tctatgctgg cgacctataa gtagcctaac atcggcgtca ccgtcgggac gagaacagcc
     1381 atttcccgcc ccggtaccgg gtgaatgatg cgtctgaccg gccggggtcc cataatccaa
     1441 agttgcgaat agtaaggggt aagccgcgtg gaacagagat tatgcagtgc ccgagggcac
     1501 atcctcccag ggacagatgt gtgacgagga gcttgcccgc tccgactgcg aggtagccgt
     1561 ccatatgact cgacgactgg gccttggcac cggggtccag tgcggactga acgagatcta
     1621 cgagcgctgg gacagcaagg gcaatccacg aaagctatcc ggcgaagaca tagccttgcc
     1681 tgcacgcttc gcccaggtcg cgagccagga ggtgctcttc gagtgcatcg gcgggccgga
     1741 cctcgccagc gagatgataa gccgacgggc cggtgctgcc ctggatcctt ccatcgcaga
     1801 aacgttcctt aaatatagtc gcgagctgct cgcggagatc atcaccgccg atgcagccgt
     1861 cgccgttatc ggggccgggc cccaccgcag gatcgcggaa cctcgcctcg acgaggtggc
     1921 gcgggctttc gcggaactgg tcgacttgaa gtcgcccttt atgcacgggc actccagctt
     1981 gaccattcca cgcactaagc aggttaggag gaggcgagga ctcgaagtaa ctccggctcg
     2041 tcagcgctca attgtggcga acctctcgcc caagaacgcg tagtactcct cgtacgtgtc
     2101 ctcttcgtag cgcccgagcc aggtgtagcg gctctgtacg ctccccgact ctcgcaggtg
     2161 ggctggcctt cgggcgtcgc gctcggcgag cagccccttc tgccactcgg cggccttctc
     2221 gtgtctgtgg ggcccgtcgg ccagctctgc gagttggcgt cccgcggggg tcttcaggac
     2281 ctctgcaaac gcgtcctcga aggttaagcc gctcccctct gaaagcgcct cggcccgacg
     2341 cgccagcgac tccaccacca tctcggaaac cgtctgctct gctctcacgc tcttctcctc
     2401 cttctacgcc cgcctgggct aatgttcgtg aatcttaagt ggccgcgcag gtcgaggccc
     2461 gcctctaata ggcacttcgg tgcatggtgg acgaccgcct ttcccctggc ggctcatccc
     2521 tcagttggta ataccaggac agctgggtgg accccacatc gtccagcaac acttcgtgag
     2581 ccgggtggtc gggatggccg aagctccggg tgatcgtgcc ggccaggccc tgcaactcgt
     2641 caggcacgga gtctaggtcg cgcaccgtca ccctcgcgcg acctttcaca ggtgccggat
     2701 ggatcaagct atctccccac gatcagggga cggcaccgag gcagccgcgt ggaagccgct
     2761 ttcaggtcct cgggccagaa cagcctgtac cggccgtcag cgagccgtac ctccacggcc
     2821 acgtacttac ggcccccgta acgacccacg atccttccca ttgcccctcg ccgggattcg
     2881 attccgtggt cgcttcgcac tcttaccctg gtgccgacgc gtgcctcctc ggaacccacg
     2941 aactcccttc cctttcccta atcgaagcga cgcgccaccc ttggaacacg tgcgctcccg
     3001 ccccctcgga cgagccaagg tgagcgacaa ccactaggtt atagtgggaa gtgacagact
     3061 tcgatgggac ttatgtccca gatgaattca tcttatccca gacctgctca tcgctgggct
     3121 ttgggaccgg gtgacttcaa atgagctgcc gtcgcgattg tcggacaggc cgaatggtcg
     3181 tgagtctacg catggaagcg acattaccag agtgggcgat tgcgctcaga tccattaccc
     3241 agcactgact cgcggcacaa ccatagcgtc gaaaatatgt cccgaggcga gcgcagagtg
     3301 gccgactcga tggtatcctc ttacgtttac agaggtagct tccgcggcgt gtgaaataca
     3361 aaggggcaat catccaacaa cctgtgccgg acgaggagtt ccatcaggga cagcagggcg
     3421 aatcggccct gcaaaacggc gaagggcatt tccatcgtct gctggagaag ttgcccgccg
     3481 gggcatacac ctgcgaccct gaagggttga tcacctacta caaccagcac gcggtgcaag
     3541 tgtggggacg agcaccgaag ctcaacgatc ccgcgtaccg cttctgcggt tccttcaagt
     3601 tgtatgcgac cgacggaacg cccatcaccc acgatcgatg ctggatggca ctggccctca
     3661 aggagaacga aggctacaac gagcgcgaga tcgtcgtcga gcgtcctgat ggacggcggc
     3721 tcaccgcact tgcgcacgcc aacccgatcc acgacgagtc aggcaacctc atcggggcgg
     3781 tgaacgtcct ggtggatata accgagcgca agcgaaccga ggaggagcta cgaatagtgc
     3841 gggaggctga gcgtagccgg attgcccgcg atctgcacga tggggtgttg caggatctcg
     3901 ccgccgtcgt tcaacgtctg gaagccacct gtatcgagca ggaagcatac tctgatggtc
     3961 gcgcacatag catcgacctt caccacgaga tcgacaccct caggcacacg gctttgaagc
     4021 tgcgccaggc ggtctattac ctgcgctcgg agaaagcccc atcgatccta agggcggttg
     4081 aggccctggt agaagtcaat ctccagaggg cacccgaatg cgacgtacgg cttgaggttc
     4141 aagacgactt cccgcacgag gttccaggcg aagcaggcag agagatgctc aggatcgttc
     4201 aggaggccct gaccaacgcc aggcgtcact ccagagcacg caacgtgcgg gtaagtttga
     4261 aagcggaagg aaccgactcc ctggtggcgg aggtatcaga cgacgggagg ggcttcgacc
     4321 tcacttcggt gaagggcggg ctggggctcg aaggcatgcg cgagcggacg actctactgg
     4381 gaggggactt gacggtctac tcggagcccg gcaagggaac ttcggtgcgc gtcaggttac
     4441 cgggactaca ggtttcacac gaggatcaag ccccggttag cgaggagagg gaggcgcgag
     4501 gttacgctag tcggactcta gctgttctag gttcacaaga ccacggcgga cggcgtatat
     4561 gacggcctgg gtcctgtcga agatgtgcag cttcttgtag atgttggagg cgtggttcct
     4621 gactgtcttc tccgagatgc ccaaatcgcg tgctatcact ttattgctct tgccctgagc
     4681 cagagcctta attacttcaa gctccctatc ggtaaggttc ggagtgagcg ctgtgttcga
     4741 acggattccc tcgaaggtcg tgatcagctt cttcgcgata tcaggggacg tgatggtatt
     4801 tccggcgtgc acggacttta tcgcgctggt caggtcttcc ggggtgcaat ccttcaggat
     4861 gtacccctgc gccccggcct tgatggcttg gaataggttg gtgtcatcgg tgtggactgt
     4921 caggaccagg atctgtgttc ttggcaaaag tccgagtatc tgtcgcgtcg cattgatccc
     4981 acccatcccc ggcatcgata ggtccatcaa tacgacgtcc ggttccttgc tcaggcacag
     5041 ttccacggcc tcctcgcccg tgtgagccct accaaccacg tccatttcag gctgggtgcc
     5101 gaggattctg acgagcccgt cgacgaagag acgctgatcg tcgacgaccg ctaccctgat
     5161 cacaccctct tcgctcacaa tgggcttcct ctcccctgcc ttctcgtggt tgtcatgtta
     5221 gcgtatcgtg ccacgtacac acatccaaat cgaggctgcc atcttttcag cagatcgaga
     5281 atccaccttc cagcaattat ctgaacaggg tcgaaaatgg gccgtttcgc ggacgcactt
     5341 tgccatgcca ggatgaccct ctcggagcgg acgaccccga tggtagcggc ttggctgctc
     5401 tcaccaacaa ccccggacag ggggaagtct gcttcaagct tggcgttgag gacatcattc
     5461 tgcccgccat cggtgcgcat agctacagtg cgcaccccgg agcgaatggg ccgatagtgg
     5521 taccgctcac accgcccgat gagaacaggg tctctggtgg atgcgcagag gtgtcggata
     5581 acgtggttgt gaagatcatc cacaaccacg agggtttcgt cgtcaacaca accgattttg
     5641 ccagcggagc gattagaggg cagttgccta atggtagaag ccgagtgagg tcctcggccg
     5701 gagtccgcgt cgaggatctt ggtgacgagc gcacttaccc cttgcgctca caaacggctg
     5761 aattcgagca ctttgaaggc tcacttgcga tcctctctgg gacatatgtt ctagggcttt
     5821 attcacccgc atcggttagt gtgtggtttg ggaaaggaaa agcgcttggg gccagcgtta
     5881 ggttctggcc ccaagtcttg agctacaagg aggcgatctt cttggaatcc catgaggcgc
     5941 gtattggcac gctggtgaga gtgttggatg gagccaggcg ggccgggaaa ggacgctttg
     6001 gcacgataga gacgacgtac ggtcatccta cctatttggc attggacgtg cggttcgaag
     6061 acggaagcgt tgagctttac tggtaccacg aactggaaag gtcgaaggtt tcttcattgg
     6121 cgtgaaacca gttgcgatgg tggcgcgtgg tgggacggac gagggcaacg gagttgtttt
     6181 gaccaacaat agtgagaggt aagctcttgg aagcgtacga agcccgtagg ggcacggtgg
     6241 tgcgtgtgcg agacgggcac tggaaatccc agtttgcggg gatgtacggc acggtccagg
     6301 cgtgctgggg gcagtcggaa cacgcggctg tggacgtttt gctagaagac ggacgttctg
     6361 agttgttctg gccgcccaac ctggagacga tagataggga tctcgccgtt tgatccttca
     6421 ccctcattca cgtggtggag cgattcagaa cgatcctggc cccgggtaca tctccacttc
     6481 ccgcatcggc gccagcccaa tcctcttcat ctccgcccgt attctcaccg aaatgtcgaa
     6541 caagcagggg tacgctgaca aaacctggca agaaactacg aaatctattc cagaggggtt
     6601 ttgcgtaacc gaagcgtatg atgtgaacaa cctcggtgcc cttactcctc ctttcccatc
     6661 atcctagagc aagggtaccg agttactccg aggggataga tttagccgaa gcgggttggg
     6721 gcgcgctaca ggagatttgt cccagttctc gacgaaggac ttgctcttgc ccaattcgtg
     6781 taacaaatcg caaggacgac gctcacgacc ttcgagggaa tccgttcgaa ccctggttcg
     6841 ccccgtcctc acctgaaggg agcatgagat gatacaggtt gaggcaagag tagcgcgccc
     6901 tgtaattctc ggagaagacc tttagaagct gccagcgtgg tgcgtttaaa gggagactgc
     6961 tacgctcacc cttgttccgt taccgggcgt gctatcgatc tgtagtctcc cgccggccca
     7021 gatggcccgc tcgcgcatgg ccgagaggcc caccccttcc cgtaccgacg cccgatcgaa
     7081 gccccgtccg tcatcagtga ccgagacctg catcatgtcc cggttcttgc gcagggctac
     7141 catgacatgc tgggcgtcgg agtggcggcg gacgttgacc agcgcttccc gaagcacgcg
     7201 cagcatctct acgtgtgcaa cgtgcggaag ttccggcggc aagccatctt gcacagccag
     7261 cgttatctcg cgctcgggtg cgagctggcg gttgaactcc accaacgcct cgacggcccg
     7321 gacgaagggc tggctcccct ccaggcgcag gtcgtagatg gcgctacgaa ggctactcac
     7381 cgcctttcgg agggcattga tctcgtggtg aaggtcgata ccacgcgccg aatccttggt
     7441 ttccgcctgg ttggcctgta gtccttgcac agccgccgcg aggtcctgga gtaccacatc
     7501 gtgcaggtcc cgcgctattc ggcgacgctc ggcctcccgg atctcgagaa gtgtctcatg
     7561 cagctgcttg ctctccgtca cgtcgcgagc agcagcgtag atcaggcctt ccttggcgac
     7621 tggaaccgct ttccactcca gccacacgta tgagccatcg gagcgacgga aacgattctc
     7681 gaaatggctg gtcgggaagt ccgtgctgag cttcgccact tcctcgacgg ctgcctctcg
     7741 gtcttcggga tggacgaaat cgacaaacgg tcttgcgagc aactctcgac tggcgtagcc
     7801 gagtgtatct tcgaacgcgg gatttacacg cttgaagtac ccgtcagaac ccaccacgca
     7861 aagtagatcg ggtgaaagcg tgaagatacg atgtaactct tcttcagccc gccgcatgcg
     7921 aagcatggct ttgatcgtcg ccacaagtac gcgctcgtcc acaggttgca ccaggtagcc
     7981 gtccgctccg ccctccagac cagtcgcctg agcctcgtaa gtcacataag tggcggagag
     8041 gtggagaacc gggatatcgg ccgttttggg gtcggctttg atccgccggc agacctcgaa
     8101 gccgtctatg tcgggcagct tcacgtcgag gataatgagg tcagcttccc tggcgagcct
     8161 gagaccctct cttccgctga ccgcctgact aaccttgaac ccttcacgct cgagggtccg
     8221 tccgatggta tagcgggtgg ttgggttgtc atctacgtgc aggatcacct tcttcgcatg
     8281 acccgtgaga ccaggcatga gctcagacat tattcggcct tcgttacggc tgatcactgg
     8341 cgaccccggc gtgcgtgata gcctccctca cgcgagcgat ggccgcttcc agcgaaaggt
     8401 tctcttttga gagaatagct atcgtttgtt cgtctagaga acttcggtcc ctatcctgta
     8461 gcggtttcga ggtaatgatg atgacaggga tgtcccgggt aaccgggtcg gatttgagcc
     8521 gctgcagcac ctcaaaacca ctcatctccg gcatcatcag gtcgagtaag acaacctgcg
     8581 gtcggttctc gcgggccatg cgcagccctt ccggtccgtt ggtggcttcg attatggtga
     8641 aagaagtctc agccaggtac cgtttcaaca cgtagcgtgc cgcttcgtca tcgtcgacga
     8701 tcaatatgct gtcagagtgt tctgtattca ctacgggagc gggcgtttgc agccggtgac
     8761 gctcaggttc cgccggcacc tgggctggcc cgctgtagac gagtgggacg acggcggaaa
     8821 agacagaccc acgacccggc gcgctctgta aggatacgct tccccctagc agctccgtca
     8881 gcctcctggc cagtggcaat cccagccccg tgcctttggt aagtcgctgt atcgggtgct
     8941 ccgcctgggc gaactcctcg aagatctggc cctgatcctc ggaggcgatg ccgataccgg
     9001 tgtcggcgac agcgaaaacg acctgctccc gcgcagcatc caaggttgcc gagacgcgca
     9061 cgtgccctcg ttctgtgaac ttgagggcat tagagacgaa attgcgcagg atctgggaaa
     9121 ccttaccttc gtcagtgtgg aggggcggga tgccgttggg ttcctcgaag tggagcgtta
     9181 cttctgggac gaccagtggc cggaagagcc cgcgcagcgt ggcgaagaga tcggccacca
     9241 ggaactcctc tggccgaacg gcgatcatgc ctgcctcgac tttcgccagg tcgagcaggt
     9301 cgttgaccag gtcggagaga tcctgggcgg cctgcctgat aaaggctatc tgcttctcct
     9361 gttcctccgt caaaaccccg tcggtacgtt cgagcaggag ccgggtaagc gccaggatgg
     9421 agttgaccgg ggtacggaac tcgtgaccca tgttcgagag gaagcgtgtc ttcaactcgt
     9481 tggcgcgctg tagctgctcc gccttctcgt ccagctcggc gtagagcgcc accacgccac
     9541 ggttggtgtc ttcgagctcg gagttcagat gggtcaattc ctcctgccgc ttgtgcagct
     9601 cgtccagggt gcgcagaagc tcctggttct gctgctgaag ctccgccgag ggcgtccggg
     9661 gcgccaactg agccagctcg ttggagatag cagcgacgtt ggcacgggcg aatgtcgccg
     9721 tgcccctcgg gatgtgcttc cccataagga ccgtcgtacc tcgcgcggga gaggattcta
     9781 tctcgaagag atccatcaga cgcttggcgc cgatgatgcc aagtcccata cccgtagagg
     9841 aacgatactg cccagctaga atctgccgca cgttttcgat cccaggccca tgatcaatga
     9901 cgcacgtaag gaaaagcggc ggatcggact tctcatcgac gcgaaactcg acccggcctc
     9961 ctcccgcata ggcgaaggca ttgcgggcga tctccgagac agcggtggcg acacgcgtct
    10021 gatcctgccg gtcgaacccg agcagtccag cggtctgccg cgctcgttgg cgcgcagtca
    10081 ccacatcgtc ctcggagtgg atcgcaacgg tgagaatggg tagagtcatc ctgttgtccc
    10141 gctcttggct tctcgggcag ccacgacggt gacatcgtct cggccgcgag tgtaatcacg
    10201 gtagagcgtg gctgcgatga tgtcggggtg atggttgagc aggccggggt agcgtctggg
    10261 atcgagatgg ctgattaacc cgtccgaata tagaaccagg agcgcatccg agaaccaggg
    10321 ataggtgaac tcctgaatcc tgcgtgccga gtgtcctgcg gtgccgttat gcgagaccat
    10381 ctggcggcta ccttcgcctg atagcacgaa gccgctgatg ttgccaacgc cggcgaagcg
    10441 tactgtctcg tgcgacgggg cgatctcggt caccgcgatc gccgccccac gtgtactgcg
    10501 caacgcggca tggatggact cgacgatcgc cgctggtgcc agagcggctc tttcgtgaaa
    10561 aacgcgttca gcttccaatg aagcttcggc ggcacccgga ccgtgcccaa ggccgtcggc
    10621 cacgaggatc atggtcctct cgggaaaccg ggccaatgcc cacctgtcac cacagacgtc
    10681 ttccccgggt ttgggcaatg acagcgcgcc aacctccaag cggggttgcc gagttgcagg
    10741 catgggcttc gaccatagac gggccaacaa cgccgtgcct acgcccggca cggagtggat
    10801 atcgaacaag gtggcgaggc gctctattgc gcccaaaccg gtgcccgggc taccggaagt
    10861 ggaatatccg tcgcgcaagg cttgggcagc gttggcgagc ccaggccctc tgtctagcgc
    10921 aagcacctgg ataccgccga tccggccgga ttgaatggcg tacagaagta tttcgccctc
    10981 tatagcatgc ttggccaggt tggttgctgc ttcggtgact atgagggcga ccttgccgat
    11041 gccagcctca tcaaaggcta gcttgctcgc aagccccgag gccgctcgac gtgtctccgc
    11101 aacctggctc atctcggtta caggcagggc caaaactctt atcacttcta ctggtttgca
    11161 gggatctctc acgtccaccg gacgatacgt actcgtgtcc cctctcccgg gtgggattcg
    11221 atctcgaact cgttcgccag ccgcctggag ccgcccaagc ccagacccag gccgccgcca
    11281 gtcgtataac cgtcccgcag cgccagctcg acgtcggcga taccggggcc gtgatcctcg
    11341 aaggtaagtt gcaggccaaa gtgggccgcg ttgcttacga ctcccatccg caccgtgcct
    11401 cctccgccat ggtttaccgt attgcgcgcc agctcgctcg ccgccgtaac cagtttggtc
    11461 tggtcgacca ggccgaatcc caggtcaatg gaccagttgc gcacagcttg ccgcactcga
    11521 actatgtctt ctgaagagcg gacgggtact atttcatcct tcgttattac catcggcgct
    11581 ctcttcagcc gttagtggca gcgacgcctg tagcagattc atacctttct cgacattcaa
    11641 cgccgtgcgg acaccgctca gggacaggcc caattctacc agcgtgatcg ccactgccgg
    11701 cctcatcccg actaccacgg tctcagcgtc gaggacccgc gacatcgcag ctatattgcc
    11761 gatcatcctg ccgataaacg agtccacgat ctccagggcc gagatgtcga taagcactcc
    11821 ctgcgcagac gtgctggcga tccggtccat caggtcattt tgaagggcga tcgccaggcg
    11881 gtcgtgcatg tcgacctgga tcgtcaccag caggaaggcg cccatcttga ggatcgggat
    11941 ccggtccacc gtgctatctc ctcgcggtct gagccaggct ctggccgctc cgctgcaggg
    12001 caaggcggaa agctgccgcc atggaagcct tcgtggtcac ctcgttgagt tccactccca
    12061 gctggacgat cgtctgtgct atctgcgggc ggatgccact gatgatgcag tcggcgccca
    12121 tcagccgggc ggcggcgacg gtcttcagca ggtgctgggc gacgagggtg tctaccgtcg
    12181 gcacgccggt gatgtcaatg atcgcgatcg tcgcttcgga ttcgacgatc tgctgcagga
    12241 ggctctccat taccacctgg gtcctggcac tgtcgagggt ccctatcaag ggcagagcca
    12301 ggatgccttc ccacagtttt acaaccggcg tcgacagctc aaggagttct tgctgctgcc
    12361 gggcaatcac cgcctctcgg ttctgctggt gaacctcggt ggtgaagagc cccaacctat
    12421 ccaacagaac cgtggccgcc cagttctcct cgacgagcgt atccgcgtga ttttcaaggt
    12481 gtcggcgcaa acgtgtgaac agaggcttct tcagtgagaa cacgaaagtg gccgtttcca
    12541 aagcggagaa gccctgttgc gcccgagagc gggagagatc ggtgaggatt tcgcgcacat
    12601 ggtcccagtg aggattctcg aatttgttta catttccgtc ctgtaatgcc tgccggaagg
    12661 cccgcaagaa ctcggccgat tgctgactta actcgaactc ggagatcaga tcggcgcgcc
    12721 tggtgtcggc ggccagttgc tcttgaaccc aatcctcgag caactcctct tcgtgatcct
    12781 tcaggatact tgggatcata ctgttttcga tcataggttg acctcccttg ggcgagcggt
    12841 aagcgccagc ggctattgta gctcaggtgg ctgtggccgg tgacctggat ctcgacagta
    12901 attgcgggac cactaacggt tcgcgcccgc tgcgcagagt ggccagagcg ctggcgacgt
    12961 ttcctctaac gcaatgttga gtatcggact cctggtagca ttgatccgct ttcgaagcac
    13021 tccgctcgtt atctgcttgc cagagtatgc tagatctact gtcggttacc ggaatacccg
    13081 tggcgcgcca ggcccgcgaa gagccccaga gggactgcca ccagtgcgat gagcagatcg
    13141 aaggcaccgg cctccgcaag cggcgccgcg gccattgtag ccaacccgct acctgagaaa
    13201 agcgcggcgg cgaagagcga gatcacggta gctcgcgcct caggcacgac ttcggtggcc
    13261 caggtctgga gagtagagtg catgtaggcg aacccccgcc gacgaatatt gccgcgatgg
    13321 tcacgtttgc cagcgactgt cccagggcgc atgaaccgta acccagcgcg agctgctcgg
    13381 agaaggtttt cggctccggc agtcgggcga gcaggacggc aggcacagca ccagcgaggg
    13441 caggagctgc ggtcctccgg aggactcttc aggcatcgcg ctctccactt tactcatccg
    13501 acagcctcca tctactgtcc cgagtggagc gtaatatccg cgagcgggct tccgcatcat
    13561 tctcgtacgg gacaatattt ccgcatctta cccacacgtt gacgcaatga agcaccgacg
    13621 agttacccag atctggtttc aaccccgaat cgcccgtcct gaaagcccga tcgcgacggc
    13681 ggcgaagacg gctaccgtag ccggaagggc gtagagtccc gccgctagca cggacaggtc
    13741 gtcgatcgct gaggcgagaa ccctctcaga gccggctctc agaagaccag ccaggagtat
    13801 cccacctccg aataccagta gcagcgcagg cagactcgat aagtcttctc ggctaggcac
    13861 gcttgccaac gcgaagctca gggacaggta cgcccagctg gtggccagca gtggggtatc
    13921 ggttagaaac ccgctcgcaa agattccttc caacgcaatc atgacggagg gtttggcgac
    13981 ctcatgaagc gtgaggggcg tgagcaacgc ggtgacgaaa aggagaccca aagggttcaa
    14041 gagtatcggc gcgagcccga taatcgctcc tacggggagg gcgcgtcgct cgtggcggac
    14101 gtatcccagc gtgaggcgac catcgttgga tctgccgggc gagaatggtg cgaagcgggt
    14161 gaccctggtt ccagtaagga gacacgccag gtagtgggag cattcgtgaa ggaccacacc
    14221 cggaaacatc aggaggtaga atgcaatacg tcccagtaca ctcccgccac caccgaatcc
    14281 tgacatcctc atcaggcgtc ccatagcgta ggaacccgcg gcgtatagga tggtcagttc
    14341 tatagcaaag agaagcagtg ctccaagcat tgctccgtcg ttcctcacgt tccggtcacc
    14401 taccttcgaa gatccacttc ggcccagatt cagtggccat gtgcctcgat gattttagaa
    14461 ccgattggag agcgtgcatc tatcctaacc tctcggtatg gttcattaac aacatgaaac
    14521 tcggcatctc gacgttggta accgacgagg ggatcgcacc gggggctctt gccggtgcga
    14581 tcgaagagcg cggcttcgac tctttgttcg tcgccgagca tacccacatc cccctcagcc
    14641 ggaagtcccc ttggccccgc ggaggagaat tgccgcgaag gtactaccgg acacttgacc
    14701 ccttcgtcac gctcaccgcc gctgccgctg cgaccgaacg cctgcttctc ggcacagggg
    14761 tagccctgat aatcgagcga gacccgatca tcacagccaa ggaagtcgcg agcctcgacc
    14821 tcgtatccgg aggacgggta gtcctcggtg tcggcgtggg ctggaatcgc gaggagatgg
    14881 agaaccacgg caccgacccg cgaacccgcg gcaagctgat ggacgagcgc atccgggcga
    14941 tgatagagat gtggacgaga gacgagccgg aataccacgg ccagttcgta gacttcgacc
    15001 cgatcggcgt ctggcccaaa ccggtgcaga agccttaccc gcccatctac gtcggaggcg
    15061 ggagccgggc cttcgagagg gtcgcccggt tcggtgacgc ctggctggca aacggacttc
    15121 cgccagataa gctcgcgccc atgatgagcg agcttcgaga agttgccggg agggacgtgc
    15181 ctgtaagcgt gttcaatgca tcc
//
LOCUS       DATAIR010000735         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_216723, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000735 DATAIR010000000
VERSION     DATAIR010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216723"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..959)
                     /locus_tag="VHM69_17540"
     CDS             complement(21..959)
                     /locus_tag="VHM69_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742247.1"
                     /translation="MNPPADVPAELRQLLDREGIEIAGSRPIDHGTQYDLTRAGETAK
                     LNVYHTGKISTGGRASGLRDLLEGWRTSQTKGESRRVSTPPTGGRPVLDGTPRLGIDE
                     SGKGDYFGPLVVAGVRITGEEEAKRLREIGVRDSKTVSVLGIRRIADLILETVGEDNV
                     CVRSLPPREYEARRRAAGNVNRLLGELDAEIIGELANEVDLVVVDEFARSARSHLEPF
                     VPEGVRLEVRTRAEDDAAVAAASIVARARQLDEMDRLSERVGFKLPLGATHVVEAGRR
                     IFEELGEEGLNEAAKTHFATTRRVLELVQHRDGGNP"
     gene            complement(956..1240)
                     /locus_tag="VHM69_17545"
     CDS             complement(956..1240)
                     /locus_tag="VHM69_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2742248.1"
                     /translation="MQNSDLERAHVRVSGQVQGVFFRDSTRKKAEELGLAGWVKNLPD
                     GKVEAVFEGPSQSVREMIRWCEEGPRHASVENVDADFESAAGDLEGFEVI"
     gene            1319..>2869
                     /locus_tag="VHM69_17550"
     CDS             1319..>2869
                     /locus_tag="VHM69_17550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2742249.1"
                     /translation="MKAEKQTVSGGTGGRSSDRTLLTLVVPTRNEADNVPKLVRELHE
                     SLSDLAYQVVFVDDSTDGTPEVIRGLAKEDGRVRLIRREGSERNGGLSTAVTTGMELF
                     SGESEYTCVMDADLQHPPEKVREMLETARTSDADVVVASRYAPGGSYVGLRGILRRAV
                     SVGSKYLAQVVFKEARKTSDPMTGFFLIKNEAISGIQFRPTGFKVLLEILVCAPELKV
                     VEAPFDFRARHAGVSKATMRQGFEYLTHILSLFWYVPSAGRFWKFALVGASGVLVNMA
                     TLIVLAEVFHAHKVIAWMFAVGISILSNFLLNNAFTWRDIRHSSRIHFFLRGALAYPV
                     AVMGIGANFAVWYPLVKYVSDDFPYYALFNLLGIIAGTSVNFILSSRLVFRPSTPRNL
                     DPMAPPKQIAEGIRRELKADRVALIRVPGLIPLGEYSPRDLNPSDRSVIDLVIQTSHP
                     TLTVTGPRRLPQARTTARWTNSLAVPILDDERVTGVVYATRTSTEPFTEEDLHWLTAY
                     TSTAAGIFE"
BASE COUNT          513 a          969 c          834 g          553 t
ORIGIN      
        1 agtgtcgctg cgaagatcta tcaagggtta cctccatctc tgtgctgtac caattcgagt
       61 acccgacgcg tggtggcaaa gtgggtcttc gccgcctcat ttagtccttc ttcccccaat
      121 tcctcgaaga tccgccgacc agcctccacg acatgcgtcg cgccgagcgg caatttgaaa
      181 ccgacccgct ccgagagccg gtccatctcg tcgagctggc gcgcccgcgc cacgatggac
      241 gcagcggcaa cagccgcgtc gtcctcggcc cgcgtgcgta cctccagccg cacaccctcg
      301 ggcacgaacg gctccagatg ggagcgggca gacctggcaa actcgtccac gactaccagg
      361 tcaacctcat tcgcaagttc acctatgatc tcggcgtcga gttcgccgag caacctgttg
      421 acgtttcccg cggcgcgccg cctggcctcg tactcccgag gaggcagaga ccttacacag
      481 acgttgtcct cacccacggt ttcgaggatg agatccgcga tcctgcgtat gccgaggaca
      541 ctgacggtct tcgagtcccg aacgcctatc tcccgcagcc ttttcgcctc ttcttcgcca
      601 gttattcgga ccccggccac gaccagagga ccgaagtagt cgcccttccc ggactcgtcg
      661 atccccagcc tcggcgtacc gtcgagaacg ggccgccctc cggtgggagg agtggaaaca
      721 cgtctactct cccccttcgt ttgcgacgtg cgccagcctt cgagcaggtc ccgcaaccct
      781 gaagctcgac ctccggtaga tatcttgccc gtatgataga cgttcagctt cgcggtctct
      841 ccggcgcggg tgagatcgta ctgggtgccg tggtctatgg gcctgctgcc tgctatctcg
      901 atgccctctc tatcgaggag ttggcgcagt tccgcgggga cgtcggcggg cgggttcaaa
      961 tgacctcgaa accttcgagg tctccggcgg cgctctcgaa gtccgcatcc acgttttcga
     1021 ccgaggcgtg acgtgggccc tcttcgcacc agcggatcat ctccctgacg ctctgagagg
     1081 ggccttcaaa gaccgcttcg accttcccgt cgggcaggtt cttcacccat cccgccaggc
     1141 caagttcttc ggccttcttc cgcgtcgagt cccggaagaa gaccccctgc acctggcccg
     1201 agacccggac gtgcgcgcgt tccagatcgc tgttctgcac ctcgtgtacc tccgtcggga
     1261 gcctttgcag tcagtctatc ccttatatgt tcacccgata tgctagccta gagcggccat
     1321 gaaagcagag aagcaaacgg tttctggcgg gacgggtggg aggagctccg acaggacgct
     1381 cctgaccctt gtcgttccga cccgcaacga ggcggacaac gtgccgaagc tcgtgcgcga
     1441 attgcacgag agcctttccg acctcgccta tcaggtggtc ttcgttgacg actctacgga
     1501 tggtacgccg gaggtgatcc ggggcctcgc gaaggaagat gggcgcgtcc gcctgatacg
     1561 ccgggagggc tcagagcgga acggcggtct ctcgacggcg gtgacgacag ggatggagct
     1621 tttctccggc gagagcgagt acacttgcgt gatggatgcg gacttgcagc accctcccga
     1681 gaaggtgcgc gagatgctgg agaccgcacg cacctctgac gccgacgtag tggtggcgag
     1741 ccgctacgcg ccaggcggca gctacgtggg ccttcggggc attttgcggc gcgcggtgtc
     1801 cgtggggagc aagtacctcg cccaggtcgt cttcaaagag gctcgcaaga ccagcgaccc
     1861 gatgacgggc ttcttcctca taaagaacga ggctatctca ggaatccagt tcaggccgac
     1921 ggggttcaaa gtactgctcg agatactggt gtgcgccccc gagctcaagg tcgtcgaggc
     1981 gcctttcgac ttcagggcac gccacgccgg ggtgtccaag gcgacaatgc gccagggctt
     2041 cgagtacctg acgcacatac tgagcctgtt ctggtacgtg ccgtcggcgg ggcgcttctg
     2101 gaagttcgcc ctggtcgggg cctcgggggt cctggtcaac atggccaccc tcatcgtgct
     2161 cgccgaggtc ttccacgccc acaaggtcat agcctggatg tttgcggtcg ggatctccat
     2221 cctgagcaac ttcctcctca acaacgcttt cacctggcgg gatatccggc actccagcag
     2281 gatccacttc ttcctgcgcg gcgccctggc ctatcccgtc gccgtcatgg gcataggggc
     2341 gaacttcgcc gtctggtatc cgctcgtaaa gtacgtctcg gacgactttc cttactacgc
     2401 gctgttcaac ctcctcggaa taatcgcggg gaccagcgtg aacttcatcc tctcctcgcg
     2461 gctcgtgttc aggccctcca cgccgaggaa cctggatccc atggctccac ccaagcagat
     2521 cgccgaggga ataaggcgcg agctcaaggc cgatagggtc gcactgatcc gtgtcccagg
     2581 actcatccca ctcggagagt attcgccaag ggatctcaac ccatcggacc ggagcgttat
     2641 agacctggtg atccaaacct cccacccaac cctgacggta accggcccca ggcgcctgcc
     2701 ccaggcccgc acgaccgccc gctggacgaa ctccctggca gtcccgatcc tcgacgacga
     2761 gcgcgtcact ggcgtcgtct acgcgacacg cacatcaact gaacccttta ccgaagagga
     2821 cctccactgg ctgaccgcgt acaccagcac cgccgcagga atcttcgag
//
LOCUS       DATAIR010000736         7840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_262375, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000736 DATAIR010000000
VERSION     DATAIR010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262375"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VHM69_17555"
     CDS             <1..181
                     /locus_tag="VHM69_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742250.1"
                     /translation="SLYLDGRPRSFAYDPATDRLTFTARRLLSYGGHTVRIVARDGAG
                     LSTTETWSFRVVRRR"
     gene            247..1620
                     /locus_tag="VHM69_17560"
     CDS             247..1620
                     /locus_tag="VHM69_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742251.1"
                     /translation="MKTFLSKTILLGRATVFTVGLAVTLALAVGAATTALAAAPGDPF
                     KLGKTNSINRLSSLVGRTAGFMLKVDNNGTGPALDLQVGPSTTPPANKTVAPMKVDSQ
                     AQVVNLNSDELDGKDSTDFARAYKRTVIVSPIPGDPSASGTALLAALSGITGASQDNP
                     YLLKLEPGLYDLGSSPLVMKQWVDIEGSGEGVTTIRRSGSPNFDTGTVVAANKSGLSS
                     LTVENTGGATRATAIWNEGATSFRLAHVTAKASGGNTNVGVDNFNASPKITDVVAAAS
                     GGSGTNNYGLYNNNSSPTIDDLEATASGGDFNCGVCNRNSSSPAMAGVIATASDGSLN
                     YGVSNHGASAPTMTQSRAFALNGSNNYGVQNTDSSPIILGSTIRASGGPDSRALTNGN
                     GVVQVDSSRLVTTPANPTVFSSGSATTKVGASLLDGGSIVNIGSNPITCAGVYDENYT
                     FFASTCP"
     gene            complement(1666..3042)
                     /locus_tag="VHM69_17565"
     CDS             complement(1666..3042)
                     /locus_tag="VHM69_17565"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2742252.1"
                     /translation="MNGLLKGALAACGGAVGAELVRRAVFSGVHPRYEPWERAPFTDF
                     PNKVLILGGGFAGYTAAKQLCKRTRNRDDVGVMVISRENFFTFWPMLAGCISSDIDTK
                     NVAQPLRRALIRDGASFRRADVESVDLDRQCVTADGREFPYDHLIVALGAEPAYFGIP
                     GVQEHAISMKGIGTAEKIRNRVIERYEETTLAHGDVPDSKLTFVIIGGGATGVETASE
                     LHELIHGVIAPDYPNINPHRTRIILVDRNEQVLKELDPALRRTARKKLAELDIQVINK
                     VKAREITAEKVVLDDGREIATENAIWTAGARASERLDDLDLPHDERRGLEVDAFMRVD
                     GHGNVWGVGDCSANVQKDGTPVPPNAQAATQEGRAVARNVLAAIDGEELQPFNYRPLG
                     QLVEMGSEFAVNEVLGIKFSGRMAALFWRMTYLYKLESPQSRARIAADWLLGRFYEPA
                     VTEIRSRE"
     gene            3148..4659
                     /locus_tag="VHM69_17570"
     CDS             3148..4659
                     /locus_tag="VHM69_17570"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX2742253.1"
                     /translation="MISNGQADTLRLLRERLATISDVYSAASVLAWDRQTYMPEGGVR
                     GRAEQLASLARLAHEMLVSPETGALLEGTGEQEPGSDEDALLRLARREHGRAIKLPAR
                     LVAETSRATALAEQAWIHAREQSDWPLFAPHMERVLALKREEAGYLDGEHPYDAMLDR
                     YEPGASTKRLQVMFDELKEGIVPFVREVSARLGEDRTAPLRGEFDEAVQDAFGRDVIS
                     SFGYDFSRGRQDRAVHPFCINFGGPGDVRITTRFDPGLLSIAFFATCHEAGHALYEQG
                     VDPSYSRTPLAGGVSMGVHEAQSRLWENLVARSRPFWSHFHPRLRRSFPGVLDDVDLG
                     TFYRAVNAVMPSEIRTVADELTYDLHVLLRFELEVALFEQDLSVEELPEAWNAKMEEY
                     LGVVPENDALGVLQDTHWAIGYFGYFPAYTVGNVLSVQLFEAAVERRPEIISEMERGE
                     FCALLGWLRENVHRHGKKYEPDELIERATGRPPDTVPYLRYLQTKFGELYGLQ"
     gene            4809..5558
                     /locus_tag="VHM69_17575"
     CDS             4809..5558
                     /locus_tag="VHM69_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742254.1"
                     /translation="MSKKWKNRLFEWLFWVSIVAGLLLMSAVFWLGWQMYSEAPEHVD
                     VGVVLATGALALFTALLWFAAAITARFARTEISTSTAVSSANLTLALDNRFNSDRALR
                     IRHGAVMCLAEKRDVHIDCDLDISPYRLDLKNRWYGLSSDLIDLFNYFDWIGYLTSDE
                     SNAIDRDVLRRKLGPWIINYYQMCEEEIDDVQQGQPDRWVHLDPLYCDLLARRKKWYS
                     DENKSLPDLDNSEELDAFLQREHVRSHRGSH"
     gene            complement(5568..6719)
                     /locus_tag="VHM69_17580"
     CDS             complement(5568..6719)
                     /locus_tag="VHM69_17580"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX2742255.1"
                     /translation="MPRIISAGDWRTLSEGLLQRGRAINAWLRAVYGGGQDLVPEEIV
                     RGSVFYRPHELPSGSPPVHIYGPDVVHLASGEYVVLEDNVRVPSGVAYSETIRRAGME
                     AIPELYEAYKVAEIFSYYDRLGETLELAAPEGVESPNVAVVTRGGDDPAYFEHRRIAE
                     ACGIAVMTLADCHVSNGVVYARDAGRRIDVIYRRFDEDYIDTDLPELERAYLEGNVGF
                     VNAPGVGVADDKAVFPYVPAMIEHYLRETPILSNAPTYSLTEEEGRAEVLDRLPELVI
                     KPREGYGAQGLVVGPEAQQDEVDTARRNIRNDPTQFVAQETLDFSTHVLGSDADKGPE
                     EVFVDLRAFVLPAIGYVVPGGLTRVAQPGTRVVNSTAGGSSKDTLVLED"
     gene            complement(7066..>7840)
                     /locus_tag="VHM69_17585"
     CDS             complement(7066..>7840)
                     /locus_tag="VHM69_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2742256.1"
                     /translation="LTALLAGAVLAFYALIGFDDSVNVAEEVRRPNRIYPRAIFGGLL
                     IAGLVYLLVTITAAMVVPISELSASDGPLLEVVERGALPIPTKFFAAIALLAVSNGAL
                     INLIMASRVVYGMGDQAVLPSVFSRVHPGRRTPWVAILFTVSIALVLLLTVGSNPDAL
                     DTLASTTVVLLLLAFVMVNVSVLALRRDPVDHEHFQTPSIFPILGITVSLALLGYQAV
                     SDISVFGLAALLLGIGLVLYVVNVFAKRSLDREPPQASE"
BASE COUNT         1488 a         2515 c         2392 g         1445 t
ORIGIN      
        1 ttccctgtac ctcgacgggc ggcccaggag cttcgcctat gatccggcca cggatcggct
       61 gaccttcacc gcgagaaggt tgctctcgta cggcggccac acggtgagga tcgtggcccg
      121 agatggagct ggcctctcta cgactgaaac ctggagcttc cgggtggtac ggcggcgatg
      181 aagtcccata aagcgacagt cccgggcgcc aggacccggc ctcaatacga agggagcgta
      241 aagctgatga agacattcct ctcaaagacg atattgctcg ggagagcaac cgtgttcacc
      301 gtgggtctcg ctgtgacgct ggctctcgcc gtcggcgcag ccacgacggc tctggccgct
      361 gctcccggcg acccgttcaa gctgggcaag accaacagca taaaccgcct cagttcactg
      421 gtagggagaa ccgccggctt catgctcaag gtggacaaca acggcacagg accggctctg
      481 gacctccagg tgggtccttc caccacccca cccgctaaca agaccgtcgc accgatgaag
      541 gtggactcgc aggctcaggt cgtcaacctc aactccgacg agctcgacgg caaggattcg
      601 acggacttcg cccgagccta caagcgcacc gtgatcgtca gtcccatccc aggagacccg
      661 tcggccagtg gcacggcgct gcttgcggcg ctctccggca taacgggcgc atcgcaggat
      721 aatccctatc tcctgaagtt agagccaggg ctctacgacc tggggagtag cccgctcgtg
      781 atgaagcagt gggtggatat agaaggctcc ggagaaggcg tcaccacgat ccgtcgctcc
      841 ggcagcccca acttcgacac aggaacagtg gttgcggcca acaagtcggg gctcagttca
      901 ctgaccgtgg agaacaccgg tggggcgacc cgcgcgacgg cgatctggaa cgagggcgcc
      961 acctcgtttc ggcttgcgca cgtgacggcc aaagcctcgg gtgggaacac caacgtcggc
     1021 gtggacaact ttaacgcatc gccaaagatc acagacgtgg tggccgctgc ttcgggtggg
     1081 agtggaacga acaactacgg gctgtacaac aacaactctt caccgacgat cgacgatctg
     1141 gaggccacgg cctcgggcgg agacttcaat tgtggcgtct gcaaccggaa ctcctcctca
     1201 ccggcgatgg ccggcgtcat tgccactgcc tcggatgggt ccctgaacta cggcgtttcg
     1261 aaccacggcg cgtcagcgcc aaccatgacc caatcaaggg ccttcgcatt gaatggaagc
     1321 aacaactacg gcgtccaaaa caccgattca tcaccgatca tattgggcag cacgatccgg
     1381 gcgagtggcg ggccagacag ccgtgcgctc acgaatggaa acggcgtagt acaggtcgac
     1441 agctctcgtt tggtcacgac tcctgccaac ccgacggtct tcagcagcgg aagcgccacc
     1501 acgaaggtgg gagcatcgct gcttgatggt gggtctatcg taaacattgg cagcaacccg
     1561 atcacctgcg caggggtcta cgacgagaac tacaccttct tcgccagcac gtgtccgtga
     1621 gcgaatacaa cgtatgccgg gacccttcag ggtcccggct cttctctact ccctgctccg
     1681 tatctcggtg acggccggtt cgtagaagcg cccgaggagc caatcggctg cgatgcgcgc
     1741 ccggctctgc gggctctcca gcttgtagag gtacgtcatg cgccagaaca gcgccgccat
     1801 gcggccactg aactttatcc cgagcacctc gtttacggcg aactcgctcc ccatctcgac
     1861 gagctgccca agtggcctgt agttgaaggg ctggagttct tcgccgtcta tggcggcgag
     1921 cacgttcctc gccacggccc tgccctcctg cgtcgcggcc tgggcgttgg gcggcacggg
     1981 tgtgccatcc ttctgtacgt tggccgagca gtcgccaacg ccccacacgt tgccgtggcc
     2041 gtcgacgcgc atgaaggcgt ccacctcgag ccctcttcgc tcgtcgtgcg ggaggtcaag
     2101 gtcgtcgagc ctctcgctcg cgcgggcgcc cgcggtccag atcgcgttct ccgtcgctat
     2161 ctcgcgaccg tcgtcgagaa cgactttctc cgcggtgatc tccctcgcct tgaccttgtt
     2221 tatgacctgt atatcgagct ccgcgagttt cttgcgcgcc gtccgcctga gtgccgggtc
     2281 gagctccttc aagacctgct cgtttctatc gacgagtatt atgcgtgtac gatgcgggtt
     2341 tatgttcggg tagtcagggg cgatgacccc gtggataagc tcgtgcagct cggaggccgt
     2401 ctctacgccc gtggccccgc cgccgatgat cacgaaggtc agcttggagt cggggacatc
     2461 gccgtgggcc agggtggtct cctcatagcg ttcgatgacc ctgttgcgga tcttctccgc
     2521 ggtcccgatg cccttcatgc tgatggcgtg ctcctggacg ccggggatgc cgaagtaagc
     2581 gggctctgcc ccgagggcga cgatcaggtg gtcgtacggg aactcccttc cgtcggcggt
     2641 gacgcactgc ctgtccagat ccacgctctc gacgtcggcg cgacggaagc tggcgccgtc
     2701 ccggatcagg gcccgcctca gaggctgggc gacgttcttg gtatctatgt cgctgcttat
     2761 gcacccggcg agcatgggcc agaaggtgaa gaagttctcc ctggagatga ccatgacccc
     2821 aacgtcgtcg cgattacggg tcctcttgca cagctgcttg gccgccgtgt acccggcgaa
     2881 cccgccgccg aggatcaaga ccttgttagg gaagtccgtg aaaggcgcgc gctcccacgg
     2941 ctcgtacctg ggatgcaccc cggagaagac ggcccgccgt accagctccg cgcccaccgc
     3001 cccgccacac gcggcgagag cccccttgag caaaccgttc atcgcaccct cccgcttcag
     3061 taggagttat ctttacattc tgatataccc gtcttcccga cgctatacgc cggaacgggc
     3121 tcgaccagcg atgctagtat tgcgcgcgtg atctcaaacg gacaggccga caccctccgg
     3181 ctgctcaggg agcgcctggc gacgatcagc gacgtatact ccgccgcctc ggtccttgcg
     3241 tgggacaggc agacgtacat gccagaggga ggtgtgcgtg gacgcgccga gcaactcgcg
     3301 agccttgccc gcctcgcgca cgagatgctc gtctcgccgg agacaggggc gctcctcgaa
     3361 ggcacaggag agcaagagcc aggctccgac gaggacgccc tcctgcgcct cgccaggcgc
     3421 gagcacggtc gggcgatcaa gttgccggcc cgcctggtag cagagacttc gcgcgcgacg
     3481 gcgctggccg agcaggcctg gatccacgcg cgagagcagt ccgactggcc gctgttcgcg
     3541 ccgcacatgg agcgggtact ggcgctgaaa cgggaagagg caggctacct cgacggcgag
     3601 catccctacg acgcgatgct cgaccgttac gaacctgggg cgagcacgaa gcgcttgcag
     3661 gtcatgttcg acgagctgaa ggagggcatc gttccgttcg tacgcgaagt ctcggccagg
     3721 ctcggggaag accgcaccgc gccgctgcgg ggcgagttcg acgaggccgt ccaggatgct
     3781 ttcggacgcg acgttatctc cagcttcggc tacgacttca gcaggggccg ccaggacagg
     3841 gccgtacacc cattttgcat caacttcggc ggtcccggtg acgtcaggat cacgacccgc
     3901 ttcgaccctg ggctgctgtc gatcgcattc ttcgccacct gccacgaggc gggccacgcc
     3961 ctctacgagc agggagtcga cccctcctac tcacgcacgc ccctcgcagg cggcgtctcg
     4021 atgggcgtgc acgaggccca gtcgcgcctg tgggagaacc tcgtcgcccg ctccaggccg
     4081 ttctggtccc acttccaccc caggttgcgg aggtctttcc ccggcgtcct cgacgacgtg
     4141 gaccttggga ccttctacag ggcggtcaac gccgtcatgc cttcggagat caggactgtg
     4201 gcggacgagc tcacctacga cctccacgtt ctcctccgct tcgagctgga ggtggcgctc
     4261 ttcgagcagg acctcagcgt cgaagagctt cctgaggcct ggaacgcgaa gatggaggag
     4321 tacctcggag tcgttcctga gaacgacgcg ctcggtgtcc tccaggacac ccactgggcc
     4381 atcggttatt tcgggtactt ccccgcctac accgtcggta acgtgctctc ggttcagctc
     4441 ttcgaagccg ccgtcgagag gcgccccgag ataatctccg agatggagcg tggcgagttc
     4501 tgcgcgctcc tcgggtggtt gcgggagaac gtccaccggc acggcaagaa gtacgagcct
     4561 gacgaactaa tcgaacgcgc cacaggacgt ccccccgata ccgtacccta cctgcgctac
     4621 ctgcagacga agttcggcga actctacggc ctgcagtaat ctcggccaga gccgtcggca
     4681 ctgtaattga tccacatcac cgatcggtcc aggcaacacg gcattcatca cggattcagc
     4741 cccaattcat catgccccct atcatcgggg aaagcgagca cctcggagta caaggagaag
     4801 tgatctctat gagcaagaag tggaagaaca ggttgttcga atggctcttt tgggtttcta
     4861 tagtagcagg gctcttgcta atgtcggctg tgttttggtt gggctggcag atgtacagcg
     4921 aggctcccga acacgtcgat gtgggtgttg tgctggccac cggagcgctg gcgctcttta
     4981 ctgctcttct ctggtttgcc gctgccataa ccgcgcgctt cgcccgtact gagatatcga
     5041 catcgactgc cgttagcagc gccaacctga cgctcgcgct ggacaacagg ttcaatagcg
     5101 atagagcctt gcgcatcagg cacggcgcag tcatgtgcct cgccgaaaag agggatgtgc
     5161 acatcgactg tgatctcgat attagtcctt accgcctcga cttgaagaac cgttggtacg
     5221 ggctcagctc ggatctgatc gatctattca actatttcga ttggatcggg tacttgacct
     5281 ccgatgagag caacgccata gacagggacg tgctgcgtag gaagctcggg ccctggatca
     5341 taaactacta tcagatgtgt gaggaagaaa tcgatgacgt gcaacagggc caacccgatc
     5401 gctgggtgca tttagaccct ctctactgtg acttgcttgc caggcgaaag aaatggtata
     5461 gcgatgagaa taaatctcta ccggacctgg acaatagtga ggaactcgat gcctttctcc
     5521 aaagagagca tgtccggagc catcgaggat cccactaatt agactctcta gtcctcaaga
     5581 accagggtat ctttggagct accgcccgct gtggagttga cgacgcgggt gcccggctgg
     5641 gcgacgcggg taagaccccc cggcactacg taaccgatcg cgggcagcac gaaagcccgc
     5701 aggtctacga acacctcctc aggcccttta tcggcatcgc tcccgagcac gtgggtcgag
     5761 aagtccagcg tctcctgggc gacgaactgc gtcgggtcgt tccttatgtt gcggcgcgcc
     5821 gtgtcgacct catcctgctg cgcctctggt ccgacgacga gcccctgcgc cccgtaacct
     5881 tcgcggggtt tgatcaccag ctcaggcaac ctatccagga cctcggcgcg gccctcttcc
     5941 tccgtaagcg agtaggtcgg cgcgttcgac aggatcgggg tctctcgtag gtagtgttcg
     6001 atcatggccg ggacgtaagg gaagacggcc ttgtcgtcgg cgacgcccac gccgggggcg
     6061 ttgacgaagc ccacgttgcc ctccaggtag gccctctcca gctcggggag gtccgtatca
     6121 atgtaatcct cgtcgaagcg gcggtagatc acatcgatcc tccggccagc gtcacgcgcg
     6181 tagacgacgc cgttcgacac gtggcaatcg gcgagcgtca tcaccgcgat accgcatgct
     6241 tccgcgatcc tgcggtgctc gaagtaagct gggtcgtcgc ctccgcgtgt gacgacggcc
     6301 acgttcgggg actccacacc ttccggcgcg gcgagttcca gggtctcccc cagccggtcg
     6361 tagtaggaga atatctccgc caccttgtat gcttcgtaaa gctccggtat cgcctccatc
     6421 cctgcccgac gaatggtctc ggagtaagcg acgccgctag gaacgcgcac gttgtcctcg
     6481 agcacgacgt actcccccga ggcgagatgg accacgtcgg gcccgtagat gtggaccggc
     6541 ggagagccgc ttgggagttc gtggggcctg tagaagacgc tgccgcgcac gatctcctcc
     6601 ggcacgaggt cctgccctcc cccgtacaca gcccgcagcc aggcgttaat ggcccgtccc
     6661 ctctgcaaga gcccttcgga gagagtacgc cagtcgccgg cggatatgat gcgtggcacc
     6721 cagtccacag gcagaatacg ttcctcatct accgtctcgt catcgacgcg gaatataact
     6781 ccctgttccc tgagttgctc cttggcgcgt ccgatccttc tccctatctc atcccgtccc
     6841 aggctccggt agagctcaag gagtggttcg tagataccac gcagagagcc gtcctcgtcg
     6901 aagatctcgt tgaaatatga aggtctcagt cggatccttc cttctgttca tctgctcgtg
     6961 aagcccgcgc tcccaggata gacgagggag cgcggctcgt atttggccct tcggagcaag
     7021 atccccgggt tcgggatctc ccacatcggc cctccgagag aacctctact cgctggcctg
     7081 cggaggctcc ctgtccagac tcctcttcgc gaacacgttt acgacgtaga gcacgaggcc
     7141 tatccccagc agcagcgccg ccagcccgaa gaccgaaata tcgctaacgg cctggtagcc
     7201 caggagcgcc agcgagaccg ttatgccgag tatcgggaag atgctcgggg tttggaagtg
     7261 ctcgtggtcg acagggtccc gccgcagagc tagtacggag acgttcacca tcacgaacgc
     7321 caacaggagg aggaccacag tagtgcttgc aagggtatcg agggcgtccg ggttggagcc
     7381 caccgtcaag aggagtacca gggcgatcga gaccgtaaac aggatcgcca cccatggcgt
     7441 gcgcctcccg ggatggaccc gcgagaacac cgaaggcaac acggcctggt cccccatccc
     7501 gtagacgacg cgggaggcca tgatcaggtt gataagcgcg ccgttggaga ccgcgaggag
     7561 cgcgatggcg gcgaagaact tggtcgggat aggcagcgcc cccctctcga ccacctcgag
     7621 caaaggcccg tccgacgccg agagctccga tatcgggact accatcgcgg cggtgatggt
     7681 gacgagcagg tagacgagtc cggcgatcag caacccaccg aagatggcgc gcggatagat
     7741 ccggttcggc cggcgcacct cctcggcaac gttgacggag tcgtcgaagc cgattagcgc
     7801 gtagaacgcc aggacggcgc ccgcgaggag tgcggtgagc
//
LOCUS       DATAIR010000737         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_262469, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000737 DATAIR010000000
VERSION     DATAIR010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262469"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..910
                     /locus_tag="VHM69_17590"
     CDS             56..910
                     /locus_tag="VHM69_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2742257.1"
                     /translation="MRTAYLTDLSDAEWSYLEPHFPAPKATGRPRLHHTRKILDAIFY
                     ILKSGCAWRLLPHDFPPWKTVHHYFRTWRIDGTWEKLNAALRERLRARIKRNTQPSAA
                     IVDSQSVKTTGVGGERGYDAGKKVKGRKRHLLVDTQGLVLKAKVHAANVFDRDGIKPL
                     LERVRERFQRLSHLWLDGGYNGKGKGKDWVEKELGLWAQVVRRPPRNRYVWVKEGEEI
                     DWERLRKLLPEPGFKVLPRRWVVERTFSWIDQNRRMSKDYERLPESSEAFIYLAMTRL
                     MVRRLARS"
     gene            907..1353
                     /locus_tag="VHM69_17595"
     CDS             907..1353
                     /locus_tag="VHM69_17595"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2742258.1"
                     /translation="MTLFGQFLRAPCNAVWASWHHVGMNAYSEDLRKKIVEAVERGMP
                     KTEAAKTFGVGISSVKRYVATARAGKSLAPKKRPGSKPKLGQGARRLLEADLEERPAA
                     TLPQRREFLGRVAGVEVSESTVSRMLKRLGWSRKKDRWERQSATSS"
     gene            1555..1932
                     /locus_tag="VHM69_17600"
     CDS             1555..1932
                     /locus_tag="VHM69_17600"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2742259.1"
                     /translation="MGPCIAVEGSTTKAVFETYVEKALAPSPRPGRVVVMDNLSSHKG
                     RRVRELIEGRGCKLLYLPPYSPDLNPIEQAFAKLKASLRRARARTFGRLIEAMGRALG
                     EITDRDASGFFRHCGYHDPAQLL"
BASE COUNT          462 a          615 c          696 g          371 t
ORIGIN      
        1 ctaagaaacc gtccgaaaag gaattctgag tcattagctc ggtaccctac cgagcatgag
       61 aacagcatac ctcactgacc tctccgatgc ggaatggtcc tacctcgagc cacactttcc
      121 tgctcccaaa gccaccggtc gccctcgact ccaccacacc cgtaagatcc tcgacgccat
      181 cttctacata ctcaagagcg gctgtgcttg gcgactacta ccgcacgact tcccaccctg
      241 gaagaccgtc caccactact tcaggacttg gcgtatcgac ggcacctggg agaagctaaa
      301 cgccgctctg cgagagcgtc tgcgtgcccg cattaagcga aatacccagc ccagcgcagc
      361 tatagtcgat tcgcaatcgg ttaagaccac cggtgtggga ggagagcgcg ggtacgatgc
      421 gggcaagaag gtgaagggaa gaaagcgcca tttgctcgta gatacgcagg gtttggtgct
      481 caaagcgaaa gtgcacgcgg cgaatgtctt cgaccgcgat gggataaagc cgcttctgga
      541 gcgcgtacga gagcgctttc aacgcctctc tcacttgtgg ttggatggtg gttacaacgg
      601 caagggcaaa ggcaaggact gggtagagaa ggagctaggc ttgtgggcgc aggtcgttag
      661 acgccctccg aggaaccgat acgtgtgggt gaaagaggga gaagagatcg actgggagag
      721 actcaggaaa ctgctgcccg agccagggtt caaggtctta ccgagacgat gggtggtaga
      781 gcggacgttt tcctggatag accagaacag gaggatgagt aaggactacg agcgcttacc
      841 tgagagtagc gaggcgttca tctacttggc aatgacacgg ctgatggtaa ggagattggc
      901 tcgctcatga cgcttttcgg acagtttctt agagcacctt gcaacgcagt ttgggctagc
      961 tggcatcatg tgggtatgaa cgcctactcc gaagacctac gcaagaagat cgtcgaggcc
     1021 gtcgagaggg ggatgcccaa gaccgaggcc gccaagacct tcggggtggg catctcttcg
     1081 gtcaagcgtt atgtcgccac ggccagagca gggaagtccc tcgccccgaa gaagcgcccc
     1141 ggctccaagc cgaaactcgg ccagggagca cggaggttgc tggaggcgga cctcgaggaa
     1201 cgaccggcgg ccaccctccc ccaaaggcgc gagttcttgg gacgagtagc cggagtagag
     1261 gtcagcgaat cgacggtgtc gcggatgctc aagcggcttg ggtggagccg aaaaaaagat
     1321 cgctgggagc gacagagcgc gacgagttct tgagggccgc ctggaaggtg ctcatggcca
     1381 gcgggatcga ggcggaacgc ttggtgttcg tggacgagtg cggcacgaac acctcgctct
     1441 gcccgctata cgcctggtcc cgaaggggcg agcgagcgta tggcgaggcg ccgcgcaact
     1501 ggggcagcaa cgtcaccctg ctggcgagca tgacgcgcga ggggatggga ccgtgtggga
     1561 ccgtgcatcg ccgtggaggg ctcgaccacc aaggccgtct tcgagacgta cgtggagaag
     1621 gcgctggccc cgtcgccgcg accggggcgg gtggtggtga tggacaacct ttcctcacac
     1681 aagggccgaa gggtgcgcga gctcatcgag ggacggggct gcaagctgtt gtacctgccg
     1741 ccctactcgc ctgacctcaa ccccatcgag caggccttcg caaagctcaa ggcctcgctg
     1801 cgcagggcgc gggccaggac cttcgggagg ctgatcgagg cgatgggtcg ggcgctcggc
     1861 gagatcaccg accgggacgc ctcgggcttc tttcggcact gcggctacca tgatccggcc
     1921 caactgctat gacagacgct ctaggcttgt cgaaggagca ctcttcccct ttctacttca
     1981 cggccatgac tgtgaggcgg ggcactgcta gtgcggatgg tatacagaac tcctcgctcc
     2041 cgacccaggc gtcgaaggct tcccggtctc tccagcgcgt gatgaccagg acctcatccg
     2101 ctccttcgga gcgtagaacc tccatcgaga cgaagcccgg gaac
//
LOCUS       DATAIR010000738         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_72206, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000738 DATAIR010000000
VERSION     DATAIR010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72206"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VHM69_17605"
     CDS             complement(<1..1798)
                     /locus_tag="VHM69_17605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX2742260.1"
                     /translation="MKTDPADLCLHEIFEEQARRTPDAPALEDASMSLTYGELDRMAD
                     RLAAYLRNAGIGPDEPVGVYMERSAEYVISCLAAMKAGGAYLILELAYPPPLLAEVVA
                     DASPRIVLTQERYADRLPDATEMFFLDDGWEEALDDVPEEDSEPEVDQDNLAFISYSS
                     GTTGKPKGIANPHRAPVRSYLWRFGISDYAPGDRVGCNVFFIWEMMRPLLRGATTVVI
                     PDDVIYDPNALIGFLEEYEVTETLVTPSLLEAVLNASGPDVGERLSKLRTLWLNGEVV
                     TRTLARRTMDLLPDARLLNVYSCSETHEVAAGDLSELVENPDSTYCAVGSLMDPERTY
                     ILDTDGELVPQGEAGELFVGGDCLARGYVNLPEKTEEGFPEDPFSSADGARMYRTGDR
                     ARILPDGSLEILGRVDFMVKIRGYSVELGAVEAAIEKDLAVSACVVVSEGEEGEDKRL
                     VAYLVPDPEDDGRYSEWSLDAKTGRSKEIRGVLQENLPHYAVPSVFVELESLPIQETS
                     GKADRSELPPPPPRPDRSTREEAESVARLPAEAPRSEKEALLARIWEAVLRLDEGEVE
                     PEDNFFDLGGHSLAAAQLSSRVEEGFGVHVSMP"
     gene            complement(1788..3413)
                     /locus_tag="VHM69_17610"
     CDS             complement(1788..3413)
                     /locus_tag="VHM69_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX2742261.1"
                     /translation="MRRSLGSAVDVSVFGISLGISVLFVLWGVLFTDSLSAVASAVLG
                     FLIEDFGWVFILSTFGFLVFVVYLAFSRYGKIKLGRDEDEPEFNTVSWVAMMFSVGMG
                     IGLMFYGVAEPISHFSAPPHGLAKAETEGAARVAMEYSYFHWALHPWAIYAVVGLALA
                     YFTFRKGRSNLISSAFYPILGDRVDGPIGKTIDILAIFATLFGTATSLGLGALQITGG
                     LNYLNDSIPADSITWAMGIIAFMTVAFILSAVSGVHRGIQWLSNVNMVLAVVLILFLV
                     VVGPTVFIFNTFTESVGGYFANLIPMSFRTAAFGDADWLAAWTIFYWAWWISWTPFVG
                     TFIARISKGRTIRQFVLGVLVVPSLVTFVWFGVLGGSALNVSLGGDAGIVNAVSESPA
                     AALFATLNEFPLAVLMSVIAMVLVALFFISGADAGAVVMGMLSSRGVLEPNRMVVVIW
                     GVLAGSAAAICLLANGLEGLQTAAIISAAPFVLVMIGLCYSLFKELSAEPSVAREPRE
                     APEPGRAAARPTGAPAPQQMRAERDPGWRQHEN"
     gene            complement(3700..>4712)
                     /locus_tag="VHM69_17615"
     CDS             complement(3700..>4712)
                     /locus_tag="VHM69_17615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2742262.1"
                     /translation="ARNEERSINESVETMLSQSYPGELEVIVIDDRSTDGTGRILRTL
                     QRANPDRLKVLRVHDLPEGWLGKNHALYLGARNAQGVWLLFTDADVRFSPLCFEYAIN
                     YASSNGLHHLTLPPEILSRGLLLRAFVAAFTLIFTVTQRPWLARDPLSRQAIGVGSFN
                     LLRSDVYEGIGTHRAIAMRPDDDMKLAKLVKKHGFRQDVVHGNGLVSVEWHESLKGAV
                     RGLGKSMFPGMDYRLDAAILASTLLFITNVLPFFSIIFSRGPARVISAPSVLLTCAMY
                     VLHARHTKSRTNLWYAALHPFSVCVFLYAMFNSVITTLANDGIEWRGTHYPLADLKRN
                     IV"
BASE COUNT          928 a         1502 c         1449 g          833 t
ORIGIN      
        1 gcggcatcga gacgtggacg ccgaaaccct cctcgacgcg gctggagagc tgcgcggcgg
       61 cgagggagtg accaccgagg tcgaagaagt tgtcttcggg ctctacctcg ccctcgtcca
      121 gccggagcac cgcctcccag atccgcgcca gcaacgcctc cttctcggac cggggagcct
      181 cagcggggag tctggcgacg ctctctgcct cttcacgggt agagcggtcg gggcggggtg
      241 gaggaggtgg gagttccgag cggtcggcct tgcctgaggt ctcctggatc gggagcgatt
      301 caagctcgac gaagacggaa gggaccgcat agtgcggcag attttcttgc aggacgcccc
      361 ttatctcctt gctccgaccc gtcttcgcgt cgaggctcca ttcagagtag cgcccgtcgt
      421 cctcgggatc gggcacgagg taggcgacga ggcgtttgtc ctcgccctcc tcaccctcgg
      481 agacaacgac gcacgcgctg acggcgaggt ccttctcgat ggcggcctca acagcgccaa
      541 gctcgacgct gtagccccgg atcttgacca tgaagtccac gcggccgagg atctccagac
      601 taccgtcggg aaggatgcgt gccctatcgc ctgtgcggta catcctggca ccgtccgctg
      661 aggagaaggg gtcctcaggg aagccctcct ccgtcttctc aggcaggttg acgtagccgc
      721 gagcgaggca gtcgccgccg acgaagagtt cccctgcctc gccttgaggg acgagctcgc
      781 catccgtgtc cagtatgtac gtgcgttccg ggtccatcag gcttccgacg gcgcagtacg
      841 tagaatccgg gttctcgacc agctcgctca ggtctcccgc ggcgacctcg tgggtctcgc
      901 tgcaactgta gacgttgagc aggcgtgcgt ctggcaacag gtccatcgtg cgccgggcga
      961 gcgttcgggt gacgacctcg ccgttcaacc aaagcgtcct gagcttcgag agcctctccc
     1021 ccacgtcagg tccgctcgcg ttcaggacgg cttccagcag ggagggggtg acgagcgtct
     1081 cggtgacctc gtactcttcg agaaagccga tcagggcatt cgggtcgtag atcacatcgt
     1141 cagggatgac gaccgtcgtg gccccacgca acagtggccg catcatctcc cagataaaga
     1201 atacgttgca ccccactctg tccccgggag cgtagtcgct gataccaaag cgccagaggt
     1261 atgaccgtac cggcgccctg tgcgggttgg ctatcccctt cggtttcccc gtcgtccccg
     1321 acgagtacga gatgaaagcc aggttgtcct gatctacctc gggttcgcta tcctcttccg
     1381 ggacgtcatc cagcgcctcc tcccagccat cgtcgaggaa gaacatttct gtcgcgtccg
     1441 gcagacgatc cgcgtatctc tcctgcgtga ggacgatgcg tgggctcgcg tcggccacga
     1501 cctcggcgag cagcggcggc gggtaggcca gctccaggat caggtacgcg ccgccggctt
     1561 tcatggcggc gaggcaggag atcacgtact ccgcgctccg ctccatgtac acgccaaccg
     1621 gctcgtcggg gccgatcccg gcgttcctga gatacgccgc gagcctgtcc gccatcctgt
     1681 ccaactcgcc gtaggtcagg gacatgctcg cgtcttcgag ggcgggcgcg tcaggggtgc
     1741 ggcgcgcctg ctcctcgaag atctcgtgca ggcagaggtc ggcagggtca gttttcatgc
     1801 tgccgccagc cagggtcgcg ctccgcccgc atctgctgcg gtgccggtgc ccccgtcgga
     1861 cgtgcggcgg cccgccccgg ttccggcgcc tcccgaggct ctctcgcgac tgagggctcg
     1921 gcgctgagct ccttgaacag cgaatagcac agcccgatca tcacgaggac gaacggcgcg
     1981 gcagagatga tggcggccgt ctgcaaccct tcgaggccgt tggccagaag gcagatcgcc
     2041 gcagccgaac cagccaagac gccccagatc accacgacca tcctgttcgg ctccagaacg
     2101 cccctcgaag agagcatccc cataaccacg gccccagcgt cggccccgct gataaagaac
     2161 aacgccacga gcaccatcgc gatgaccgac atcaggacgg ccagcgggaa ctcgttgagg
     2221 gtggcgaata gggcagcggc cggactctct gagacggcgt tcacgatccc ggcgtcgccg
     2281 cccagcgaca cgttcagggc cgagccgccg agaactccga accacacgaa ggtgacgagg
     2341 ctcggcacca cgagcacgcc gaggacgaac tgccttatgg tgcggccctt ggagatgcgg
     2401 gcgatgaacg tcccgacgaa cggggtccag gagatccacc acgcccagta gaagatggtc
     2461 caggcggcga gccagtcagc gtcgccgaag gccgccgtac ggaagctcat cgggatgagg
     2521 ttggcgaagt atccaccaac agactccgtg aaggtgttga agatgaagac cgtgggaccc
     2581 acgacgacga ggaagaggat gagcaccacc gcgagcacca tgttgacgtt cgagagccac
     2641 tgtatccccc tgtgcacacc ggagaccgcg gaaaggatga aagccaccgt catgaaagcg
     2701 atgatcccca tggcccaggt tatggagtcg gcggggatgg agtcgttcag gtagttgagc
     2761 ccgccggtga tctgcaacgc cccgagcccg agcgaggtcg ccgtcccgaa gagcgtcgcg
     2821 aagatcgcca gtatgtctat ggtcttgccg attgggccat cgaccctgtc gccaaggatc
     2881 gggtagaagg ctgagcttat gaggttgctg cgccccttgc ggaaggtgaa gtaagcgagc
     2941 gcgagcccga cgacggcgta gatggcccac ggatgcagcg cccagtggaa gtacgagtac
     3001 tccatcgcca cccgcgcagc accctccgtc tccgccttcg caagcccgtg cggcggggcc
     3061 gagaagtggg agatgggctc cgcgacaccg tagaacatca gcccgatccc catacccacg
     3121 ctgaacatca tcgcgaccca cgagacggtg ttgaactcgg gctcgtcctc gtcacgccct
     3181 agctttatct tcccgtagcg cgagaacgcc agatacacaa cgaacaccag gaacccgaac
     3241 gtcgagagga tgaagaccca cccgaagtcc tcgatcagga acccgagcac cgccgaagct
     3301 acagccgaga ggctgtccgt gaagagcacc ccccacaata cgaaaagcac cgagatgcct
     3361 aaagagatac cgaacacgct gacgtctacc gccgagccta atgaccttcg cataacagac
     3421 caccctctcc ttacgcttcc ttcattgaca acgcgttgca tcgatccatc cttggtgcgg
     3481 caggacgccg catagaaatg atgcggacca tgctcaggtc gatgaccctg agcgaagcgg
     3541 cccggaaacg ttggttgaca tactaacccg ccggaacgtt cattccagcg cttaccccct
     3601 agataagttt aaagttgtat taagtttcgt taaggacaga tgaatttccc aggttcgcgg
     3661 gcctggcgcg ggctaccctt ggcacctctt gcggggagat cacacgatgt tcctcttcag
     3721 gtccgccagg gggtagtgag ttccacgcca ttcgatcccg tcgtttgcga gcgtcgtgat
     3781 gaccgagttg aacatggcgt agagaaagac gcacacgcta aacgggtgga gcgcggcgta
     3841 ccacaggttt gttctcgact tcgtgtgtct ggcgtgaagg acatacatcg cgcaggtaag
     3901 aagcacgctc ggggcggaga tgaccctcgc cggcccgcgc gaaaagatga tgctgaaaaa
     3961 cgggagtaca ttggtgatga aaagcagtgt agaagcaagg atcgccgcgt ccaacctgta
     4021 gtccatgccg gggaacatgc tcttgccgag accgcggaca gcacccttca ggctctcgtg
     4081 ccactcgacg ctcacgaggc cgttcccgtg gacaacgtcc tgtctaaatc catgcttctt
     4141 gaccagcttc gcgagcttca tgtcgtcatc cgggcgcatg gctatggcgc ggtgggttcc
     4201 gattccttcg tacacgtcac ttctcagcaa gttaaaggac cccacgccga tggcttgcct
     4261 tgatagtgga tcccgtgcca gccacggcct ctgggtcacg gtgaagatca gggtgaacgc
     4321 ggccacgaac gccctcaaca gcaagcctct ggagaggatc tccggcggca gcgtcaggtg
     4381 gtggagcccg ttgctagaag cgtaatttat ggcgtactcg aaacaaagag gtgagaaccg
     4441 tacgtcggca tcggtgaaca ggagccaaac cccctgcgcg ttccttgccc cgaggtagag
     4501 agcgtggttc ttccccaacc agccttcagg tagatcgtgt acccggagga ccttcaacct
     4561 gtccgggttc gcccgttgca gcgttcggag gattctcccc gttccatccg tggagcggtc
     4621 gtcgatgacg atgacttcga gttctcccgg atagctctgg gacaacatcg tctcgacgct
     4681 ctcattgatg gatctctcct catttcgggc tg
//
LOCUS       DATAIR010000739         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_355413, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000739 DATAIR010000000
VERSION     DATAIR010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355413"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..742)
                     /locus_tag="VHM69_17620"
     CDS             complement(185..742)
                     /locus_tag="VHM69_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742263.1"
                     /translation="MIVQFSFWTAGLVQTLTPVLLHGLIYGAVLSALMGVIFLAIAYA
                     NPEIWLNDYPPDIRERFGPMSERAKRQRRLAGVPVFMLLFGTIVLSALRLARIGDDEV
                     FFAVFFGTFIMLLVFNLVDLLILDWLVFNTLRPGFVVLPGTEGARGYSDYGFHFRAFL
                     KGVAGALVVSFIVAGAASVISTFAA"
     gene            complement(895..1644)
                     /locus_tag="VHM69_17625"
     CDS             complement(895..1644)
                     /locus_tag="VHM69_17625"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2742264.1"
                     /translation="MKIIGAGFGRTGTTSLKAALEELGFDPCYHMTEVFAHPEHADFW
                     KAAWRGDNVDWEGVLGGYEAAVDWPACTFYRELMQRYPEAKVLLSVRDPEPWYKSTRG
                     TIYELSMLVEKSTLPRLIFGAISLLIFGGFAGGRSGMANDIIWQGTFDGRFEDKDYAI
                     EVFERHNEEVKRSVPPSRLLVYDVREGWGPLCEFLGVDEPEEPFPRLNDTAQMRRGML
                     TIRALSIALPAALALSVAAALVLLRRRAGKP"
     gene            complement(1645..3270)
                     /locus_tag="VHM69_17630"
     CDS             complement(1645..3270)
                     /locus_tag="VHM69_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2742265.1"
                     /translation="MLEGLAQDFQLTLPYILRRAEQLYGYREIVTRRPDKSFHRHTFA
                     DFVSRAKKLAVALDNLGLEPGDRVGTLGWNTYQHLEAYFGIPTSGAVLHTLNPRLHED
                     DLSYIANHAEDRVMLVDETLVPVFQKFREKTGVEHAVVMTEGGEIPEGMISYEELLGD
                     ADESSFEYPEFDEKQASAMCYTSGTTGRPKGVLYSHRSIALHSMMAAMGNTFGLSEPD
                     CVLPVVPMFHVNAWGLPFASVLVGAKLMMPGPHLDPASLLEDLEQEEVTLTAGVPTVF
                     LAVLQALDEEPEKYDLSKLRSMVIGGSAVPKGVIKAYGERHGIDILHAWGMTEMDPIG
                     TVANLTTEIEKLPEEEQLEYKAKQGYPVPFVEIRARGDEGLVPWDGESLGELEVRGPW
                     VARSYYEAPEGDDKFTDDGWFTTGDVVTIDRFGFIKITDRSKDLIKSGGEWISSVELE
                     NALMAHPAVSQACVIAIPHEKWDERPLAAIVLKEGESATEEDLHSHLEKDFARFWLPD
                     AYEFVESIPMTATGKFQKLKLREQFEGYTSTKN"
     gene            3412..3600
                     /locus_tag="VHM69_17635"
     CDS             3412..3600
                     /locus_tag="VHM69_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742266.1"
                     /translation="MVEEESFKVVAVTLFGKIVVARYPTLEQAEWRARELNQEAERSP
                     RGYIQYMVQPSEGPSGRG"
BASE COUNT          666 a         1120 c         1188 g          697 t
ORIGIN      
        1 ggacgttctt ttggaccagg atctctgttg tgcgtatcat catcctccca gactcggttc
       61 gggtaattta ccaggaggat agtgcaaacg gggcagggct tcgctgtgtg gggagcgaca
      121 tcgaggttcc ctcttttcgc ggacgacccc ctcggtgacg tctcgattct acgaccagag
      181 gatctcaggc cgcgaaggtg ctgatcaccg aggctgctcc ggcgacgatg aagctcacga
      241 caagagcccc cgccacgccc ttcaagaacg ccctgaagtg gaagccataa tcgctgtagc
      301 ccctcgcgcc ttcggtgccg ggcaagacga cgaaccctgg tctgagcgtg ttgaaaacaa
      361 gccagtcgag gatcaacagg tcaaccaggt tgaagacaag caacataatg aaggtgccga
      421 agaaaacagc gaagaaaacc tcatcatcgc cgatccgcgc gagtcgaagg gccgacagga
      481 cgatggttcc gaacaggagc atgaagacgg ggacgccggc cagcctgcgc tgtcttttgg
      541 ccctctcgct catcggtccg aacctctccc tgatgtccgg cgggtagtcg ttcaaccaga
      601 tctcggggtt ggcgtatgcg attgccagaa agatcacacc catcagcgcg ctcaggacgg
      661 cgccgtagat caggccgtgc agcagcacgg gagtcaaggt ttgtacgagt cccgccgtcc
      721 aaaacgagaa ctggacgatc atggctttct cctctggtcg gggcggaacc tccagggtct
      781 cgcttcgaga tcggataact gctgatctat cctacgacgc ctccacccct tcgaagccga
      841 gtattctggg cggtcgccgg tcgaaggagg tgcgtcgagg ttggcttcga ccggctacgg
      901 tttgcctgcg cgcctgcgga gcagtacgag agccgccgcg accgagagcg ccagcgctgc
      961 cggcagagct atggagagtg cccggatcgt cagcatcccg cgccgcatct gggccgtgtc
     1021 gttgaggcgc gggaaaggct cctcgggctc gtcgacgccg aggaactcgc acaacggccc
     1081 ccaaccctct ctgacatcgt agaccagcag ccgcgagggt gggacgctgc gtttgacttc
     1141 ttcattgtgc cgctcgaaga cctcgatggc gtaatccttg tcctcgaagc gcccgtcgaa
     1201 ggtgccctgc cagattatgt cgttcgccat cccgctccga ccccccgcga aaccgccgaa
     1261 gatgaggagg ctgatcgcgc cgaagatgag gcgcggaagg gtggactttt cgacgagcat
     1321 gctcaactcg tagatggtgc cgcgggtgct cttgtaccac ggttccggat cccgcacgct
     1381 caggagtacc ttcgcctcgg gataccgttg catgagctcc cggtagaagg tgcaggcagg
     1441 ccagtccacg gcggcctcgt agcccccgag gaccccttcc caatccacat tgtctccgcg
     1501 ccaggccgcc ttccagaaat ccgcgtgctc ggggtgcgcg aagacctccg tcatgtggta
     1561 gcaagggtcg aagccaagct cttcgagcgc cgccttcagc gacgtcgtgc ccgtccggcc
     1621 gaagccggct ccgatgatct tcacttagtt cttcgtggac gtgtagcctt cgaactgctc
     1681 gcgcagcttc aacttctgga acttgcccgt ggccgtcatc gggatggact cgacgaactc
     1741 gtaggcgtcc ggcagccaga acctggcgaa gtccttttcg aggtgactgt gcaggtcctc
     1801 ctccgtcgcg ctctcgccct ccttgaggac gatggcggcc agaggtcttt cgtcccattt
     1861 ctcgtggggg atggcgatga cgcaggcctg ggagacggcg gggtgggcca tgagggcgtt
     1921 ctcaagctcg acggaggaga tccactcccc cccgctcttt atgaggtcct tgctgcggtc
     1981 tgtgatcttg atgaatccga acctgtcgat ggtgacgacg tcccccgtcg tgaaccaccc
     2041 gtcgtccgtg aacttgtcgt ccccttcggg cgcctcgtag taggacctcg cgacccaggg
     2101 accacgaacc tccagttcgc cgagcgattc gccgtcccag gggaccaggc cctcgtcccc
     2161 gcgggcgcgt atctccacga aaggcacggg gtagccctgc ttggccttgt actcgagttg
     2221 ctcctcttcg ggcagcttct ctatctccgt ggtcaggtta gcgacggtcc cgatgggatc
     2281 catctccgtc atgccccagg cgtggaggat gtcgatcccg tgccgctccc cgtacgcttt
     2341 tatgacgccc ttggggactg cagatccccc gatgaccatc gagcggagct tggacaggtc
     2401 gtacttctca ggctcctcgt cgagcgcctg caggacggcg aggaagacgg tgggaacgcc
     2461 cgcggtgagc gtcacctctt cctgttcgag gtcctcgaga aggctcgccg ggtcgaggtg
     2521 gggtcccggc atcatcagtt tcgccccgac gaggacggag gcgaagggca gtccccaggc
     2581 gttgacgtgg aacataggca cgacgggcag cacgcagtcc ggttccgaga gcccgaaggt
     2641 gttgcccatc gccgccatca tcgagtgcag ggcgatggag cggtgcgagt agaggacgcc
     2701 cttggggcgg cccgtggtac cggaggtgta gcacatcgcg gaggcctgct tctcgtcgaa
     2761 ctcagggtac tcgaagctcg actcgtccgc gtcgccaaga agttcctcgt aggagatcat
     2821 accttcagga atctccccgc cctccgtcat gaccacggcg tgctcgacgc ctgttttctc
     2881 cctgaacttc tgaaacacgg gaacgagggt ctcgtctacg agcatgaccc tgtcctctgc
     2941 gtggttggcg atgtaggaaa ggtcgtcctc gtgcaaacga gggttcaggg tgtgcagcac
     3001 ggcccccgag gttgggatgc cgaagtacgc ttcgaggtgc tggtacgtgt tccagccgag
     3061 cgtgccgaca cggtcgccgg gttcgagccc gaggttgtcc agggcgacgg ccagcttctt
     3121 ggcgcgggag acgaaatcgg cgaaggtgtg gcggtggaag gacttgtcgg ggcggcgggt
     3181 gacgatctcg cggtatccgt agagctgctc cgcccgccgc agaatatagg gcagggtaag
     3241 ttggaagtcc tgggcaagtc cctctagcat ctcgtctcct ctataggtag ggggccgggc
     3301 gcgcctgcgg cgcgccccga acgcctgtac tccctctccc ggaaagcatt gttaagggcg
     3361 atcttagcag gcacaatgaa agggggtcaa ggctagaggc gggtgcctgg catggtggaa
     3421 gaggaaagtt tcaaggttgt cgcggtgacg ctcttcggga agatagtggt ggcccgctac
     3481 cctacactgg agcaggccga gtggcgggcc agagagctga accaggaggc cgaacgcagc
     3541 ccgcgcggct acatacagta catggtgcag ccatccgagg ggccctcggg gaggggatga
     3601 cgcggccgcc gtggtggcag gtcacgaagt ttgatctgga cagggcggcg gcgatgcgct
     3661 agcttactcg a
//
LOCUS       DATAIR010000740         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_263294, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000740 DATAIR010000000
VERSION     DATAIR010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263294"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VHM69_17640"
     CDS             complement(<1..971)
                     /locus_tag="VHM69_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742267.1"
                     /translation="MRKVRWGILGTGAIARQFVEGLGHLPEAEVLAVGSRSEASAAEF
                     ADEREIPRRYASYDDLVADPDVDVVYVATPHPFHAGNAERCLEAGKAVLCEKPFTLNA
                     AEAERVVWLARDRGLFLMEGMWTRFFPLMEKLRRLVSEGAIGEVRMLNVDFGFRADLD
                     PASRLFDPKLGGGALLDVGVYCVSLASMVLGKPSRAVGLSHLGETGVDEQASVVLEHE
                     GGRLANLSIGIRTATPQEVTIMGTDGYIRVHSPWWRPTSMTIERPGERTETVEAPVSG
                     NGFNYEAAEVMRCLEAGETESYVMPLDETLSVMRTMDGIRAAWGLRYP"
     gene            1196..2206
                     /locus_tag="VHM69_17645"
     CDS             1196..2206
                     /locus_tag="VHM69_17645"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2742268.1"
                     /translation="MEYRNLGRTGVKVSELCLGCMMFGGRTGEEDSADIIDRAIDAGV
                     NFLDTANVYSTGASEEAVGKALKRNGKRDSIVLATKVHGVMDENDPNAGGNHRRHIIE
                     QCEASLKRLQVDHIDLYQIHRPSSEVPIDETLRALDDLIRAGKVRYVGTSTFAAWQLV
                     EAMWAARELGLNRFVTEQPPYHLLDRRIERELVPFARTYGVALIPWSPLAGGFLTGKY
                     SRDESQSPDDSRYGIDPRRMGRNHFTDEAFDVLDAVQTIAGEKGCTVGQVALAWCKDQ
                     PGITSPIIGPRTMEQLEDNLGTVEVSLDENDHERLDAVAPPGRATVPYYQADFGPHRF
                     RW"
     gene            complement(2214..>2488)
                     /locus_tag="VHM69_17650"
     CDS             complement(2214..>2488)
                     /locus_tag="VHM69_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742269.1"
                     /translation="MEEERVPEHSGTNHSMAIGFYIAAILLIFGVTIALTGIIAPAQQ
                     TAGDLGFNVNLWWGLVMVVSGCVVLGMTLASPRRRAAQRQAQQQSE"
BASE COUNT          464 a          847 c          760 g          417 t
ORIGIN      
        1 ggatacctga gaccccatgc ggcgcggatc ccatccatcg tcctcatgac cgagagcgtc
       61 tcgtcaagcg gcataacgta gctctctgtc tcgccagctt cgaggcatcg catgacctcg
      121 gcggcctcgt agttgaagcc gttgcccgag acgggggcct cgacggtctc ggtcctttca
      181 cctggccttt cgatcgtcat cgacgtcggg cgccaccatg gactgtgtac cctgatgtat
      241 ccatcggtac ccatgatggt cacctcctgt ggcgtcgccg tccggatccc gatggacagg
      301 ttggcgagcc tccctccttc gtgctccagg accacagacg cctgctcgtc cacccctgtc
      361 tccccgagat gcgagagccc cacagccctt gaaggctttc caaggaccat tgaggcgagt
      421 gagacgcaat agactccaac gtcgagcagg gcgccgccgc cgagcttcgg gtcgaagagc
      481 cgggaagctg ggtcgagatc cgccctgaaa ccgaagtcca cgttgagcat ccgcacctcg
      541 ccgatagccc cctccgagac cagccgcctc agcttctcca tcagcgggaa gaacctggtc
      601 cacataccct ccatgaggaa gagccctctg tccctggcga gccagacgac cctttcggcc
      661 tcggcggcgt tgagggtaaa cggcttttcg cagagcacgg ccttgcccgc ctccagacaa
      721 cgttcggcgt tgcccgcgtg aaatgggtgt ggtgtcgcga cgtagacaac gtcgacgtct
      781 ggatcggcga cgaggtcatc gtaggaagcg tagcggcgtg ggatctctct ttcatcggcg
      841 aactccgcgg cggaggcttc ggagcgggac ccgacggcga ggacctctgc ttcagggagg
      901 tggcccagtc cttctacgaa ctgcctcgcg atggcgccgg tccccagtat cccccaccgg
      961 accttcctca cgatgtcccc cggaggcggc tcgtcagggg cgcggcgtac cggagcgcga
     1021 cggcccccga gcctccccca agctctgtat gttcgtcgcg ggccgtgctc gtccttccaa
     1081 ttcgcggtac aggccgcgat gctaccactt cggtgcatgg aatcaaaccg gacgggttgc
     1141 acggacatca ggctgcctgt tgaatatgtc caccactacc tcgaaggagc gaatcgtgga
     1201 gtacaggaat ctcggaagga caggcgtcaa ggtcagcgag ctctgcctcg ggtgcatgat
     1261 gttcggggga cggaccggcg aagaagactc ggcggatatc atcgaccgcg ccatcgacgc
     1321 gggcgtcaac ttccttgata cggcgaacgt ctacagcacc ggcgcaagcg aagaggcggt
     1381 ggggaaggcg ctgaagagga acggcaagcg cgactccatc gtcctcgcga ccaaggtgca
     1441 cggggtgatg gacgagaacg atcccaacgc gggcggcaac cacaggcgcc acatcatcga
     1501 gcagtgcgaa gcttcgctca agcgtctaca ggtcgaccac atagacctgt accagatcca
     1561 ccgcccgagc tccgaggtcc ccatcgacga gacgttgcgg gctttagacg acctgatccg
     1621 cgcgggcaag gtgcgctacg tgggcacttc gaccttcgcc gcctggcagc tcgtcgaggc
     1681 gatgtgggcc gcaagagagc tcgggctcaa ccgcttcgtc acagagcagc ccccctacca
     1741 cctccttgat cgtcgcatcg agcgcgagct agtccccttc gcccggactt acggggtggc
     1801 cctgatcccc tggtcccccc tcgcaggcgg cttcctgaca ggcaagtaca gccgcgacga
     1861 gagccagtcc cccgacgata gccgctacgg catcgatccc aggcgcatgg gccgcaacca
     1921 cttcaccgac gaggccttcg acgtactcga tgcggtacag accatagcgg gcgagaaggg
     1981 ctgtaccgta ggtcaggtcg cgctcgcatg gtgcaaggac cagcccggca tcaccagccc
     2041 catcatcggg ccgcgcacca tggagcagct agaagacaac cttggcaccg tggaagtctc
     2101 cctcgacgag aacgaccacg agcgcctcga cgccgtagcg cccccgggac gggccacggt
     2161 tccctactac caggctgact tcggcccgca ccgcttccgc tggtgagaag cccctactcg
     2221 ctctgctgct gcgcctgccg ctgtgccgcg cggcggcggg gcgaggcgag ggtcatcccc
     2281 aggacgacgc atcccgagac gaccatcacc aggccccacc acaggttcac gttaaagccg
     2341 aggtcacccg cagtctgctg cgccggggcg atgatcccgg tcagggcaat cgtgacgccg
     2401 aagatcagga ggatggccgc gatatagaag cctatcgcca tggagtggtt tgttcccgag
     2461 tgctccggca ccctctcctc ttccatgt
//
LOCUS       DATAIR010000741         6157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_72394, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000741 DATAIR010000000
VERSION     DATAIR010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72394"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VHM69_17655"
     CDS             <1..343
                     /locus_tag="VHM69_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742270.1"
                     /translation="SPWTIYSQVPDLAFLQRPLTAFAIFLAVLVAFVPRKKTLRRLAA
                     LSAAIVIAFQLTVNYWFYSYVIWFEPFVFLALLLSTNEKTALDGRHQASGISDQQDRE
                     VGDVRDGKVPG"
     gene            321..923
                     /locus_tag="VHM69_17660"
     CDS             321..923
                     /locus_tag="VHM69_17660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2742271.1"
                     /translation="MTEKCQDNKEPILNVVGELVALGPLRRDLLPVYQRWINDLGTMR
                     NLGVAPHPMTHEKEQDWYDRQSKTEDEVPFTIYERETLRPIGNTGLHDIDHRNRSATF
                     GILIGEPECRGRGYGTETTSLMLDYAFTALGLHNVMLTVFEFNQAGIRAYEKAGFREF
                     GRRRQCRMTGGKLRDEIFMDCLSSEFESPVLRRIYAPDEE"
     gene            913..1434
                     /locus_tag="VHM69_17665"
     CDS             913..1434
                     /locus_tag="VHM69_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742272.1"
                     /translation="MRNRVLETERMVLRRMEMSDVDHLMGIFSDPVAMRYYPGTKSRK
                     EVEEWVRWTLASYRDHGFGLWVAILKDSGGFAGQCGLTMQEVEGREEVEIGYHVLREF
                     WGRGLATEAATAVRDHGFQALGIERLVSLIDPLNLSSRRVAEKTGLTLEKEVWKWNKN
                     ICVYAISKDENVP"
     gene            complement(1676..2116)
                     /locus_tag="VHM69_17670"
     CDS             complement(1676..2116)
                     /locus_tag="VHM69_17670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2742273.1"
                     /translation="MHIEVQTQNVDGKVTSLGTAEPFTLVVDRPTEGGGGGLGFNGGQ
                     LLYLAVAGCISNDLFREARTSGIELSGVRVKVSGDFVGEPAVSDEIQYEVEVSGDAPE
                     DRLRALIDQVDEIAEIPNSLRNGTRVRLIGAKVGHTPYGGSRKG"
     gene            complement(2165..3460)
                     /locus_tag="VHM69_17675"
     CDS             complement(2165..3460)
                     /locus_tag="VHM69_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX2742274.1"
                     /translation="MDLFDESGIENLASRAPLAERLRPATLDEVVGQPHLTAEGGPLR
                     SVVERGRIGSVVLWGPPGTGKTTLARVLASTVEEESIPLSAVTSGVKDLRAAMEGARE
                     RLKYEGRGTLVFVDEVHRFNKAQQDALLPALEEGLVDFIGATTENPSFEVTAPLLSRS
                     RVLRLRSLSEDDLGLLLDRGMDDLGVGVSDDAREYLLRLAGGDGRRMLNALEVAAAGT
                     KQVEVADVERAVGQRALRYGREEHYDVISAFIKSVRGGDPDAALHYLARMLEAGEDPV
                     FVARRLVILASEDIGNADPHGLTLAVSAAEAVRLIGMPEGRIPLAQATTYLASAPKSN
                     AAYAGISAALSDVRRENTPEVPMHLRNAVTGLMKEEGYGANYRYAHGDEPEGMNDRYL
                     PEELADRVYYEPEKSGEEAGIRDRLQRWREARERRETEK"
     gene            3555..4823
                     /locus_tag="VHM69_17680"
     CDS             3555..4823
                     /locus_tag="VHM69_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742275.1"
                     /translation="MTTGARHQDRRVGVLGARASSWLAWSLCLMCVAPLAVGAILVPV
                     NGTSSDVGTWGTANLIFIFPVVILSFALVGTLIASRLPTNPIGWVCLGIGLILTLAIV
                     TGEYYVYALQTRPGSLPGGVYMAWLTNWLWVPAVVSIGTFLVLLFPDGRLPTRRWRLV
                     AWLCVAVLISTSASQAFSPGPLAEAPNVTNPLGIEAASGALELANTVSFLLLPLCFVL
                     SALSMIVRFRHSIGVERQQIKWFATAAALLAIVFSSSFVVPARIVQDLVTLLFAGLPI
                     AVGVAILRHRLYDIDVVINRALVYGSLTAMLAALYLGGVATTQTIFRALTGQEQQPQL
                     AIVASTLVIAALFNPLRRRIQSFIDRRFYRRKYDARKTLEAFSARLRDETDLHALNGE
                     LTRVVRETMQPVHVSVWLRPDVGPKDGPAN"
     gene            4873..5346
                     /locus_tag="VHM69_17685"
     CDS             4873..5346
                     /locus_tag="VHM69_17685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742276.1"
                     /translation="MIEIVPYEPGLHREGIREILARNGWEGRYIAGQLDKLDVLSGDP
                     KPGTRGRVYVSESDGVLAGFVSIELREWNRLGQLHGLAVDPSLKRRGIASALVSHAEV
                     FVREKDGRGIYVDTPATNEVARRFYLALGYHLVYTMPEYYDEGLDGVTYLKLFPK"
BASE COUNT         1119 a         1958 c         1838 g         1242 t
ORIGIN      
        1 cagcccctgg accatctact cccaggtccc ggatctcgcg ttcctgcaac gaccgctgac
       61 ggcgttcgcg atcttcctcg ccgtactcgt cgccttcgtg ccgcgcaaga agacgctgcg
      121 ccggctcgcc gccctctccg ctgctatcgt catcgccttc cagctcaccg tcaattactg
      181 gttctactct tacgtcatct ggttcgaacc cttcgtcttc ctcgccctgc tcctctccac
      241 caacgagaag accgcgctgg acggaaggca tcaggcatca ggcatcagcg atcagcaaga
      301 tagggaggtt ggtgacgtcc gtgacggaaa agtgccagga taacaaggag ccgatcctga
      361 acgtcgtggg cgagctggtg gcgctcggtc cgctacgaag ggacctcttg ccggtctacc
      421 agcgttggat caacgacctc ggcacgatgc gcaacctggg ggtcgcgccg catcccatga
      481 cgcacgagaa ggaacaggac tggtacgaca ggcagtcgaa gacggaggac gaggtgccgt
      541 ttacgatcta cgaacgggag accttgcgcc ccataggcaa cacgggacta cacgacatag
      601 atcaccgcaa ccgaagtgcc acattcggga tcttgatcgg cgagcccgag tgccgcggca
      661 ggggttacgg taccgagacc accagcctga tgctcgacta cgctttcacg gccctcggcc
      721 tgcacaacgt catgctgacg gtcttcgagt tcaaccaggc cggcatcaga gcttatgaga
      781 aagccggttt cagggagttc ggcaggcggc ggcaatgccg catgacgggg gggaagctga
      841 gggatgagat cttcatggac tgcctcagca gcgagttcga gagcccggtc ctgcgccgga
      901 tctacgcgcc ggatgaggaa tagagtactg gagacggagc gtatggtgct ccgtcggatg
      961 gagatgtccg acgttgacca cctcatgggc atattctccg atcccgtggc gatgcgctac
     1021 taccctggca ccaagagccg caaggaggtg gaggagtggg tgcgctggac gcttgcgagt
     1081 tacagagatc acggcttcgg cctctgggta gcgatcctca aagattcagg cgggttcgct
     1141 ggtcagtgcg gactcaccat gcaggaggtc gaaggtagag aagaggtcga gatcggctac
     1201 cacgtcctgc gagagttctg gggccgcggc ctggcgaccg aggctgcgac ggcagtaagg
     1261 gaccacggct tccaagctct cgggatcgag cggctcgtct ccctcataga cccacttaac
     1321 ctctcctccc gccgcgtcgc cgagaagacc ggtctcacgc tggagaagga ggtctggaaa
     1381 tggaacaaga atatctgcgt ctacgcgatc agcaaggatg aaaacgtgcc gtaaacaggc
     1441 acgcgctctg tctacatgcg cccttgaaaa ccgcttgtgc ttcccgtcag gattgccgcg
     1501 cgagccgatt ctcaccctcc gcaatggtgg gaagagcaag gtgtgcctat tgtgcgtcga
     1561 caatagcctg aaatagagcg gtttgcggaa agagtgaacc tgaggtgagt aaaggtttca
     1621 ggctacaggc atcagcaaaa gacctgaagc ctgatgcctt ctccgaagga gtagctcatc
     1681 cttttcgaga accgccatag ggggtatggc ccaccttcgc tccgatcaga cgcacgcgcg
     1741 tcccatttcg gagcgagttc gggatctcag ctatttcgtc gacctgatct atcagcgccc
     1801 gcagccggtc ctcgggagcg tcgccggata cttcgacctc atactggatc tcatctgaga
     1861 ccgcaggctc acctacgaag tctccgctca cttttactcg gacgccgctc agttcgatgc
     1921 cggacgtccg ggcctctcgg aagaggtcat tggagatgca cccggcgacc gcgagataca
     1981 gaagttgtcc gccgttaaag cccaatcccc cgcctccgcc ttctgtgggg cgatctacca
     2041 ccagcgtgaa tggctctgcg gtaccgagtg atgtgacctt gccgtcaacg ttctgtgtct
     2101 ggacttcgat gtgcatgtgt tctcctcccg ccaccccttg catcacctga tttcgcaacg
     2161 aactctactt ctccgtctct cgccgctctc tcgcctcccg ccagcgttgc agccgatccc
     2221 tgatccccgc ctcttccccg ctcttctccg gctcgtagta gacgcgatcg gcgagctctt
     2281 ctggcaggta cctgtcgttc attccctcag gctcgtcgcc gtgggcgtag cggtaattcg
     2341 cgccgtagcc ctcttctttc atgagtcccg taacggcgtt gcgcaggtgc atgggtacct
     2401 ctggcgtgtt ctcacggcgc acgtcggaga gcgccgcgct aatgcccgcg taggccgcgt
     2461 tggatttggg agccgacgca agatacgtcg tcgcctgcgc gaggggaatt cgcccctcgg
     2521 gcatcccgat gagcctgacc gcttcggcgg ctgagacggc gagcgtcagg ccgtgcgggt
     2581 cggcgttgcc gatgtcttct gaggccagga tcacgagccg ccgcgcgacg aacacggggt
     2641 cctcccccgc ctccagcatc cttgcgagat aatgaagcgc ggcgtcgggg tcgccgccgc
     2701 gtacgctctt gatgaacgcg ctgatgacgt cgtagtgctc ctcgcgcccg tacctgagcg
     2761 ccctctgccc cacagcccgc tcgacgtcag cgacctcgac ctgcttagta cccgccgccg
     2821 cgacctccag ggcgttgagc atccgccgcc catcaccgcc ggcgagccgc agcaggtact
     2881 cgcgagcatc gtcagagacc ccgaccccga gatcatccat cccccggtca agtaagagac
     2941 cgaggtcgtc ctccgacagg gaccggagcc tgagcacccg cgagcgggag agcagtggcg
     3001 ctgttacctc gaaggaagga ttctctgtcg tcgcccctat gaagtccacg agcccctctt
     3061 cgagggcggg gagcagggcg tcctgctgag ccttgttgaa gcggtggacc tcgtcaacga
     3121 agacgagcgt ccctcgtccc tcgtatttta gccgttcgcg ggcaccttcc atggcggccc
     3181 tcaggtcctt caccccgctc gtcacggcgc tcagggggat ggactcctct tcgaccgtgc
     3241 tcgccagtac ccgcgccagg gtcgtcttcc ccgtgccggg aggtccccac aggacgacag
     3301 acccgatcct gccgcgctcc acgaccgacc tcaagggtcc gccctcggcc gtcaggtgcg
     3361 gctggccgac gacctcatcg agcgtcgctg ggcgcagccg ctcggcgagc ggggcgcggc
     3421 ttgccaggtt ctcgatgccg gattcgtcga agagatccac atggttagta tatccttcgt
     3481 cacttcgaat ccctcccttg cttcgtaacg cctgccgtaa cgactgggag acggtagtcc
     3541 tgtagagtgg tgtcgtgaca actggagcaa ggcatcaaga tcgcagggtc ggtgtgctag
     3601 gcgcccgcgc ctcctcgtgg ctggcctggt cgctgtgcct gatgtgcgtg gcacctcttg
     3661 cggtgggtgc gatcctagtg cccgtgaacg gcacaagctc agacgtgggc acatggggca
     3721 ccgcgaacct gatcttcatc tttccggtgg tgatcctctc tttcgcgctc gtgggaacct
     3781 tgatcgcctc tcggctcccc accaacccca tcggttgggt gtgcctcggc atcggcctca
     3841 tcctgacgct cgcgattgtg acgggcgagt actacgtgta cgcgctacag acccgacccg
     3901 gttccctccc cggtggtgtg tacatggcgt ggctgacgaa ctggctctgg gtacccgcgg
     3961 tggtctcgat cgggaccttc ctggtactgc tcttccccga cgggcgcctg cccactcgcc
     4021 gctggcggct tgtggcgtgg ctctgcgtgg cggtgctgat ctcgacgagc gcctcccaag
     4081 cgttctctcc tggccctctc gcagaggccc cgaacgtcac gaacccgttg ggcatagagg
     4141 ccgcgagtgg tgccctggag ctggccaaca ccgtgagctt cttgctcctg ccgctctgct
     4201 ttgtcctgtc ggcgctctcc atgatcgtac gcttcagaca ctccatcggg gtagagcgcc
     4261 agcagatcaa atggttcgct accgccgcgg ctctcctggc gatagttttt tcctcctcgt
     4321 tcgtggtgcc cgcgagaata gtccaggacc ttgtgaccct cctcttcgca ggcctcccca
     4381 tagcggtagg cgtggcgatc ctacgccacc gcctctacga catagacgtg gtcataaacc
     4441 gcgccctggt ctacggctcg cttacagcga tgctggccgc actctacctc ggaggcgtgg
     4501 cgacgaccca aactatattc cgtgccctaa ccggccagga acagcagccg caactcgcta
     4561 tcgtcgcctc aactcttgtc atcgcagccc tcttcaaccc tctgaggcga cgcatccagt
     4621 cgttcataga tcgtcgcttc tacaggcgta agtacgatgc cagaaagacc ctggaggcct
     4681 tctcggccag gctgagggac gagacggact tacacgcgtt gaacggcgaa cttacgagag
     4741 tggtgcggga gacgatgcag ccggtacacg tctccgtgtg gctgcgcccg gatgtcggac
     4801 cgaaagatgg gccggcgaat tagcaacgaa ctcgaagact gttcaggtca tcaacggagc
     4861 tgtgtaggtg gcgtgatcga aatcgtccct tatgaacccg ggctccaccg ggaaggcatc
     4921 cgtgagatcc tggcgagaaa cgggtgggag ggcaggtaca tcgcgggcca actcgacaaa
     4981 ctcgatgtcc tctcgggaga tccgaagccc ggaacccgtg ggagggttta cgtcagcgag
     5041 tcggatggcg tcctcgcagg cttcgtcagt atcgagcttc gagagtggaa tcgtcttgga
     5101 cagttgcacg ggctcgcggt ggacccgagt ctcaaacgcc ggggtatcgc ttccgccctt
     5161 gtcagccacg ccgaagtgtt cgtgagggag aaggacgggc gtggcatcta cgtcgacact
     5221 ccggcgacca acgaggtcgc tcgccgcttt tacctggccc tgggatatca cctcgtctac
     5281 acgatgcctg agtattacga cgagggactc gacggcgtca cctatctcaa gctgttccca
     5341 aaatgaggaa ccatctcact ttccgtaagc ccatccgaaa ggagcgagga aacgtagcaa
     5401 gcggttgcga cttgctacct tccaacttcc cgagactttc aggaatcctt gttaggagtc
     5461 tgctctagcc tggatgcctt gtattgcccc ccgaagcgtc gagatctcgc cctcgattga
     5521 ggaacgggac ctactcacat cggagccagc tttgtttgaa tcgcgtgtcc tggcagactc
     5581 aacggcgtcc tcaatcaaca gtcgcatggg gtcagcctcc ggcaaggatt gcgccaactg
     5641 ctccagttgc gttaggtagc gctctagatc cgcttggtct cctgccgcca ggcgacgctc
     5701 caggtcactc agtaggtctc gtgcctgctc ggctttgcgt agctcttcga tcatctcctc
     5761 gagcggtgct ggcatagccc ttacctcctt ttcagtttgc gccgcctggg gcgccgcgca
     5821 tcaatctgag cttcttccgc ataggaccgg ggctgtccac tttccaccca acgtcaggga
     5881 aggttgactc ttggaagttg gatactgttg ttactcctga gagtcttcct gtcccacttc
     5941 ctcgatctgt ttgaggcctt cgaccatctc gaacgtgaag cgcaccgttc ggcatacgcg
     6001 cgcgatgacg gggagggcaa taatggagtc cgggaaagga aacaaacgca accttgggta
     6061 gagactaaga agctcgccga catcgagatg ggcgcctgtc gagggcgagc agagtcagtg
     6121 gcgcaaccgg ctacactccg caggaggtgg gccggtt
//
LOCUS       DATAIR010000742         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_263667, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000742 DATAIR010000000
VERSION     DATAIR010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263667"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VHM69_17690"
     CDS             complement(<1..465)
                     /locus_tag="VHM69_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX2742277.1"
                     /translation="MADMDTQAGTPADHRHVPVRSDTFELHGIHCLCCAAAVERALRE
                     QPDVTEVRLDWKNDLVHVGYDPARIGQEDIDKVITQTGCDCKLIGGEEHHESVADPER
                     RMRHLEHGVDAQPISMGTKHDRMQYEMPATPADHGDHMDHDMSDPAMAKAMER"
     gene            complement(551..1966)
                     /locus_tag="VHM69_17695"
     CDS             complement(551..1966)
                     /locus_tag="VHM69_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742278.1"
                     /translation="MSTQIILRVLALRHRLRQRDRWTRRRLEAHQARELRLLREHAYT
                     RSPFYGNFHKGLSARPLQDLPILTKEMVMEHFDELVADPAVRLDDVEAHLATLSGGDE
                     LFDGRYRVASTSGSTGRRGLFLWDPGEWATVLASYNRSFDWAGVGAGLTHRTSMVVVS
                     STTPWHQSARVGASVHSPWVPTLRIDSGDPLEIIVDRLNGFQPRVLVAYASMAHLLAE
                     EQLAGRLRISPGFVFGSSEVFTEQAKRRVEEAWGRKPFEVYAATEPAGIASECDLHRG
                     MHLFEDLVITEMVDEKNRPVPPGVYGEKVLVTVLFSRTMPLIRYEMSDSVRPASSPNC
                     PCGRPFHLIDGIQGRAEDVLRFPSASGGQVAVQPIVFHRVMDTVPAGGWQIAQGSDGL
                     TVLLSGVREGFADATLVDSLRRELATQGALAPPVKVRRVQAIPRTTVGKAPLIKSDVR
                     TSTLHQSRPCSDSPPRPGTNR"
     gene            complement(1983..2669)
                     /locus_tag="VHM69_17700"
     CDS             complement(1983..2669)
                     /locus_tag="VHM69_17700"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX2742279.1"
                     /translation="MKTILTKIWLLSILVAVAFILTACGDAGSGQQGRGSDGDGERRN
                     AKGAGMDHDQMGRGSMGMGSGGMAKQMVMENGKYSDRRFIDAMVPHHQGAIEMGKVAL
                     KNAEHEEIKELSRNIVSTQQSEIEELKSIKKEEFGSSSVTMEMSPEQMRDMGMMMDPQ
                     QLVHSGPFDKAFIDAMIPHHQSAIDMATVANEKSDNLHIKELAENIVSAQKREIEQMK
                     RWREQWYSED"
     gene            complement(2741..3115)
                     /locus_tag="VHM69_17705"
     CDS             complement(2741..3115)
                     /locus_tag="VHM69_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742280.1"
                     /translation="MELRGTTQLSVLLIVTLLFCHGALGGLHLVCLLPGCADHGHYAA
                     KYQPTTGGLGDAHEHPAGHETSIEYFAVLVGLLGLLLRLLPKLAPSRGGLSTPRAEAL
                     RRLPVAFHLPSPKPPNLQVFTL"
     gene            complement(3353..3562)
                     /locus_tag="VHM69_17710"
     CDS             complement(3353..3562)
                     /locus_tag="VHM69_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2742281.1"
                     /translation="MTKTTFQVPDMSCGHCKAAVEGELNKLFGVESSNADVDKSTVEI
                     NYDEGTVTTEELKGAIEEAGYTVAA"
     gene            complement(3562..3828)
                     /locus_tag="VHM69_17715"
     CDS             complement(3562..3828)
                     /locus_tag="VHM69_17715"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2742282.1"
                     /translation="MLENRLKQIEGQMRGVQRMLVEEDYCVDVFTQIASVVSALEKVD
                     TILLKDHIDHCVRASIAKGEYSEERIRELIAAVERFFRAYRKER"
BASE COUNT          680 a         1203 c         1129 g          845 t
ORIGIN      
        1 gcgctccatc gctttcgcca tggcagggtc tgacatgtcg tggtccatgt ggtcgccatg
       61 atccgctggg gttgcgggca tctcgtactg catgcggtcg tgctttgttc ccatagagat
      121 gggctgcgca tctacgccgt gttcgaggtg ccgcatgcgc cgctctgggt ccgccactga
      181 ctcgtggtgc tcctcacccc cgataagttt gcagtcgcag cccgtctgcg tgatgacctt
      241 gtcgatatct tcctggccga tcctcgcggg atcgtagccg acgtgtacga ggtcgttctt
      301 ccagtccagc cgaacttcgg tgacatccgg ctgctcccgt agggctcgct cgacggccgc
      361 agcgcagcac agacagtgga tgccgtggag ttcgaacgtg tcagaccgca cgggaacatg
      421 acgatgatcg gcaggtgtcc cagcctgagt atccatatcg gccatggtgg ctaactcctc
      481 cttagaactt cgggtctcac gtcgatctgc aaaccgtcga ctgccgcttc gactttctca
      541 gttaccatcc tcacctgttc gtccctggcc gaggcggcga atctgaacat ggtctcgact
      601 ggtgcagggt actcgtgcgt acgtcggact tgatcaaggg tgctttgcca acagtggtgc
      661 gggggatggc ctgcacccgc cgtaccttga ccggcggtgc gagtgctccc tgggtcgcga
      721 gctctcgccg aagcgagtcg accagggtgg cgtccgcaaa gccttcacgc acgccgctca
      781 gcagcacggt gagcccatcc gatccttgag ctatctgcca tccgccagcg ggtacggtat
      841 ccatcacgcg gtggaacact ataggttgca ccgcgacctg tccccccgac gcggaaggga
      901 accgaagcac gtcctctgct cgcccctgga taccgtcgat gagatggaag ggacgcccgc
      961 aggggcagtt gggagaagag gcaggcctca cgctgtcgct catctcgtaa cggataaggg
     1021 gcatggtgcg ggagaagagg accgtcacga gtaccttctc cccatacacg ccgggaggaa
     1081 cgggcctgtt cttctcatcg accatctcag ttatgacgag gtcctcgaag aggtgcattc
     1141 cccggtgcag atcgcactcg gaggcgatcc ccgcgggttc ggtcgccgca tacacttcga
     1201 atggcttccg gccccacgcc tcctcgacgc gccgcttggc ctgctcggta aagacttcag
     1261 agctaccgaa gacgaagccg ggagagatcc tgagacgccc tgcgagttgc tcttctgcca
     1321 ggaggtgcgc catcgaggcg taggctacga ggaccctggg ctggaagccg ttgagcctat
     1381 ccacgatgat ctctaacgga tcccccgagt ctatgcgcag ggtagggacc caggggctgt
     1441 gcacagaggc gcccaccctc gccgactggt gccagggtgt tgtagaactc accaccacca
     1501 tactcgtgcg atgcgtgagg ccggcgccaa cgccggccca gtcgaaggag cggttgtagg
     1561 aggcgagcac ggtggcccac tcgcccgggt cccagaggaa gagaccacgt ctgccggtgc
     1621 ttcccgaggt ggaggccacc ctgtagcgcc cgtcgaagag ctcgtccccg ccgctcagcg
     1681 tagcgaggtg ggcttcgacg tcgtctagcc gcacggctgg atccgcgacg agttcgtcga
     1741 agtgttccat gaccatctcc ttggtcagga tcggcaggtc ttggagtggt cgtgcgctaa
     1801 gccccttgtg gaaattcccg tagaacggag agcgggtgta ggcgtgctcc cgcagcagtc
     1861 ggagctccct ggcctggtgc gcctcgagcc tccggcgcgt ccagcgatcc ctctggcgca
     1921 ggcggtggcg aagagccagg accctgagga ttatctgcgt actcatcgca tctctcccag
     1981 ccttagtctt ccgagtacca ttgctcccgc cagcgcttca tctgctcgat ctcccgcttc
     2041 tgggcgctca cgatgttctc tgcaagctcc tttatgtgca ggttatcgct cttctcgttg
     2101 gctacggtcg ccatatctat ggccgactgg tggtgcggga tcatggcgtc gatgaacgcc
     2161 ttgtcaaagg gcccgctgtg gaccaactgc tgcggatcca tcatcatgcc catgtcccgc
     2221 atctgctccg ggctcatctc catcgttacg ctcgaactgc caaactcctc tttcttgatg
     2281 gatttgagct cctctatctc ggattgctgg gtcgagacga tgttccgaga gagctccttt
     2341 atctcctcgt gctcagcgtt cttgagggcc accttgccca tctctatggc gccctggtgg
     2401 tggggaacca tcgcgtcgat aaaccgcctg tcggaatatt tgccgttctc cataaccatc
     2461 tgcttggcca tcccaccgga gcccataccc atcgagccgc ggcccatctg atcgtggtcc
     2521 atcccggccc ctttagcgtt gcggcgctcg ccatcaccgt cggatccacg accttgctgg
     2581 ccactgccag cgtcgccgca ggcggtcaag atgaaggcaa ccgccacgag tatcgagagc
     2641 agccagatct ttgtcagaat tgtcttcaag acaaccccct gttcgtgcgt tgttcggatg
     2701 aagtaccttg ctcccgcatt atcgggacga gtagatttat tcagagcgtg aatacctgaa
     2761 ggttaggcgg tttaggggat ggtagatgga atgcgacggg caaccggcga agcgcctcgg
     2821 ctcggggcgt gctgagtccg ccacgcgacg gcgctaactt cgggagcaac ctcaagagca
     2881 ggccgagtaa tccgacgagc acggcgaagt attcgatgct agtctcatga cctgcggggt
     2941 gctcgtgcgc gtcacccagc ccaccggtcg taggctgata ctttgcggcg taatgtccgt
     3001 ggtcggcgca ccctggaagt aagcacacta ggtgcagacc cccgagggcg ccgtggcaga
     3061 agagcaacgt gacaatgagc aacacggata actgcgtggt gccgcgcaac tccatcagtg
     3121 atccactttt gtcttactcg acatgggcga cggaatttta cggttcggcc aaggtgatcg
     3181 tatatctacc ctatccaagg cgactatcct cttcgcgagt cgctacggag cttcaaccgg
     3241 tgtccggttc cagtgttaag gattgtgtcg cggcaggcgt tactattcat gacaaatatt
     3301 gttgttatgg ctggccaatc ttgatcaacc tagcgaccgt ggcactgacc tcctatgcgg
     3361 ctacggtgta gccggcctcc tcgatggcac ctttgagctc ctcggtggtc accgtaccct
     3421 cgtcgtagtt gatctcgacc gtgctcttgt ccacgtccgc gttggagctc tccaccccga
     3481 agagcttgtt cagttcgccc tcaaccgcag ccttgcagtg gccgcagctc atgtccggaa
     3541 cctggaatgt cgtcttggtc atcatctctc ctttctgtaa gccctaaaga agcgctcaac
     3601 ggcggcgatt agctccctta ttctctcctc agagtactcg cccttcgcta tggacgccct
     3661 gacgcaatgg tcgatgtggt ccttgagcag gatggtgtcg accttctcca acgccgagac
     3721 gacgctggcg atctgtgtga atacgtctac gcagtagtcc tcctcgacca acatgcgctg
     3781 cacgccgcgc atctggccct ctatctgctt gaggcggttc tcgagcagct ccttttgcgg
     3841 ttatgctata ctcccta
//
LOCUS       DATAIR010000743         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_264639, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000743 DATAIR010000000
VERSION     DATAIR010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264639"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VHM69_17720"
     CDS             complement(<1..439)
                     /locus_tag="VHM69_17720"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX2742283.1"
                     /translation="MSGDPAGTTLKPDRLFDPGRRSVASLLYEEVENLPLVCPHGHVP
                     PALLADPDATLGSPAELFIIPDHYVFRMLYSQGVSMEDLGVPTVDGTPVETDHRHIWQ
                     RFCEKFHLFRATPTGIWLSDELVNVFGVTEKPGQENAQRIYDHL"
     gene            complement(436..1482)
                     /locus_tag="VHM69_17725"
     CDS             complement(436..1482)
                     /locus_tag="VHM69_17725"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742284.1"
                     /translation="MADFGVGIIGAGTISKVHARAIDRIETTRLTAVAEPREDAGREL
                     AGEYGAEWHADPSALLGREDVDVVVLCTPSGLHADHTVAAAGAGKHVITEKPMAITLE
                     SIDRMILACREAGVSLSVIFQYRFNREALRLKRALDAGLFGRPVIGNALVHWHRTQEY
                     YEEKGGWRGTWALDGGGALMNQSVHAIDLLQWLLGPVESLSAYTETLTHDIETEDVAS
                     ATVRFTSGAMGAIQGTTSTHTDYPLKIELRGTQGSATFEGARLTDWRPSEEEEVLSEE
                     EQLTFPDNPDEPFGAAHERQLRAIFAALDEGSEPPIPGEEARKAVEIILGIYRSAREG
                     EQVSLEPAIDGRPS"
     gene            complement(1519..2367)
                     /locus_tag="VHM69_17730"
     CDS             complement(1519..2367)
                     /locus_tag="VHM69_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2742285.1"
                     /translation="MKVGVFTVGLPDLTPEEAVREIKDARYDGVEWRVTRVHDEVKGE
                     EPSFWGNNLCTLRPTDKEAHRARRLSEEVGLEVPGLGTYVAVGDLEAADEAMRFAVTA
                     GAPQVRVGAGALDGSYEESFCSAREFLGGVEEMAGSHGVKALVEIHHKTICPSASLAH
                     RLVSAFDPERIGVIFDPGNMAQEGFEDYRIGLELLGPYLAHVHIKNSAFERPEGGGVW
                     KPGWAPLEDGVVDFEFLFAALDSVGYDGWLVIEDFSAARPTREALRHNLGFVRTLAEG
                     SRAGKA"
     gene            complement(2417..>2654)
                     /locus_tag="VHM69_17735"
     CDS             complement(2417..>2654)
                     /locus_tag="VHM69_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2742286.1"
                     /translation="LKNRPDLTAVLDVTHPEPPEPDSPLYDLSNVVLTPHIAGSQGDE
                     CRRMGRLVVDELRRYVAGEPLEHEITKERAAVMA"
BASE COUNT          414 a          887 c          837 g          516 t
ORIGIN      
        1 ggagatggtc gtagatcctc tgcgcgttct cctggccggg cttctcagtg acgccgaaga
       61 cgttcaccag ctcgtccgag agccagattc cggtcggggt ggcgcggaag aggtggaatt
      121 tctcgcagaa gcgctgccag atatgccggt ggtccgtctc cacgggggtg ccgtccaccg
      181 tgggcacccc gaggtcctcc atcgacacgc cctggctgta gagcatcctg aagacgtagt
      241 ggtcgggaat gataaataac tcagccggcg agccgagggt cgcgtccggg tcagcgagca
      301 aggccggcgg gacgtggccg tgggggcaga ctagcggcag gttctcgacc tcttcgtaga
      361 gcaggctggc cacggaacgc ctgcccgggt cgaagagcct gtcgggcttt agcgtagtcc
      421 ccgcgggatc gccgctcaag atggcctccc gtctatcgct ggctccaggg agacctgttc
      481 tccctcgcgg gcggagcggt agatgccgag gatgatctcg acggccttgc gggcctcttc
      541 gcccggtatg ggcggctcgc tcccctcatc gagcgccgcg aagatcgcac gcagttgccg
      601 ctcgtgcgcc gccccgaagg gctcgtccgg attatcgggg aaggtcaact gctcttcctc
      661 cgataggacc tcttcctcct cggacgggcg ccagtccgtc aggcgcgcgc cctcgaaggt
      721 cgcactgcct tgcgtgccgc gaagttcgat cttcagaggg tagtcggtat gcgtcgatgt
      781 tgtgccctgg atagcaccca ttgccccgct cgtgaagcgc acggttgcgg aggcgacgtc
      841 ctctgtctct atgtcgtgcg tgagggtttc tgtgtaggcg ctgaggctct cgacggggcc
      901 gagtagccac tgcaggaggt cgatggcgtg cacgctctgg ttcatcaggg caccgccacc
      961 gtcgagtgcc cacgtgcccc tccagccgcc cttctcctca tagtactcct gggtgcggtg
     1021 ccagtgtacg agcgcgttgc caatcacagg gcgtccgaag agccccgcat cgagggcgcg
     1081 cttcaggcgg agcgcctcgc gattgaagcg gtactggaag atcaccgaca gcgacactcc
     1141 ggcctcgcgg caagccagga tcatgcggtc gatgctttcg agtgtgatcg ccatcggctt
     1201 ctccgtgatg acgtgctttc cggccccggc tgcggcaaca gtgtggtcgg cgtgcagccc
     1261 cgagggggtg cagagcacca ccacgtcaac gtcctccctg ccgagcaggg ctgaagggtc
     1321 ggcgtgccat tcggccccgt attctcctgc cagttccctg cccgcatcct cccgcggctc
     1381 ggcgactgcc gttagccggg ttgtctcgat cctatcaata gcccttgcgt ggaccttgct
     1441 gatcgttcca gccccgatga tgcctactcc gaagtctgcc atgcgtctcc catccatgcg
     1501 tccgtagggc cgcgtcctct aagcctttcc tgcgcgggac ccttcggcga gggtccgtac
     1561 aaagcccagg ttgtgcctca gagcctcgcg ggtcggtcgg gcggcgctga agtcttcgat
     1621 caccagccac ccgtcgtagc cgacgctgtc gagcgcggcg aagagaaact cgaagtccac
     1681 caccccgtct tcgagcggcg cccagcccgg cttccacaca ccaccgccct cgggtcgttc
     1741 gaaggcggag ttcttgatgt ggacgtgcgc aagatacggc cccagtagct ccagcccgat
     1801 acggtagtcc tcgaagccct cctgtgccat gttgccagga tcgaagatca caccgatcct
     1861 ctcggggtcg aaagctgaga cgagccggtg ggccagcgag gcgctcgggc aaatggtctt
     1921 gtgatgaatc tccacgagcg cctttacgcc gtgggatcct gccatttcct cgacacctcc
     1981 caggaactcc ctggccgagc aaaacgactc ttcgtaagac ccgtcgagtg cgcctgcccc
     2041 tacacgtacc tggggagctc cggcggtcac cgcgaagcgc atggcttcat cggcggcctc
     2101 gaggtcgccg acggcgacgt aggtgcccag ccccggcacc tcgagcccta cctcctccga
     2161 caggcggcgt gcccggtggg cttccttgtc ggtgggccgg agggtgcaga ggttgttccc
     2221 ccagaacgag ggttcttccc ccttgacctc gtcatggacc ctggtcaccc gccactcgac
     2281 gccgtcgtat ctggcgtcct ttatctcgcg cacagcctcc tcgggcgtga ggtcaggcag
     2341 gccaactgta aagactccta ctttcataga ccctctcttc ggtcgtctgg tctacgtcca
     2401 ttcgggcttc gttcgctcag gccatcacgg ctgccctctc cttagtgatc tcgtgctcca
     2461 acggctcccc cgccacgtac cggcgaagct cgtccacgac gagtcggccc atgcgccggc
     2521 actcgtcacc ttgagagcct gcgatgtggg gtgtgagcac gacgttggac aggtcgtaaa
     2581 ggggagagtc cggttccggc ggttcaggat gcgtcacgtc cagaacagcc gtgaggtccg
     2641 gccgattctt gagc
//
LOCUS       DATAIR010000744         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_264673, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000744 DATAIR010000000
VERSION     DATAIR010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264673"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1098
                     /locus_tag="VHM69_17740"
     CDS             43..1098
                     /locus_tag="VHM69_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2742287.1"
                     /translation="MEYRQLGHSGLFVSAMTLGTMTFGGRGDFGVVGSTDVATATRQV
                     DMCLDAGVNLFDTADMYSDGESEEILGQAISGRRDRLLLATKARMPVGEGPNDAGLSR
                     HHLIRQCEASLRRLGTDYIDLYQAHEWDGVTPLEETLDALDTLVRSGKVRYVGCSNHS
                     GWQLMKALGVSERLGLHRYVSQQIYYSLEARDAEYELVPLAMDQDCSILVWSPLAGGL
                     LTGKYRRDQDASEGRHVSGWDEPPVRDPEKLHDTVDTLVEIAESHGVSAAQVALAWLL
                     GRPGVASVVVGARTEEQLADNLAAAELRLSEEERRRLDEVSAPPLLYPYWHQARTASD
                     RLSPADLTLLGPHIADA"
     gene            complement(1118..1987)
                     /locus_tag="VHM69_17745"
     CDS             complement(1118..1987)
                     /locus_tag="VHM69_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2742288.1"
                     /translation="MAKMRRPIVPYVRDASFPVRSAWYTAPRRLLDYLLMYVQEGEMI
                     ARVEGRGHRFSEGDFCLIQPGELHTLEGPANTITPYVHLDVFYNQAREHSFVVRSGRV
                     DVSGLGELMQPRLFEETGIRVPTRFVPQNPAEFRDTMLKTIGIWQRRDLLGQLESHYL
                     ATGLVLSVLQSYSRRRADSLDQPEFLNWITSFMSLHLSEALSVSDMAERAGLSASRFS
                     QVFRDRFGRPPHQFLLHLRVQRAQDLLQHTGLTMREISGQCGFSDVHHFAKTFKRLSG
                     QTPGSYRANQPSG"
     gene            2259..>2645
                     /locus_tag="VHM69_17750"
     CDS             2259..>2645
                     /locus_tag="VHM69_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2742289.1"
                     /translation="MYGRVSRKQFLVRGGKAALGAGLAGSLLAACGGGGGGSGQVTYW
                     SNLEGSGPQDYFKKNIEKPFEKSQDGTDLKVTFQPPDEMDRLVRTALQGGEGPDIIMT
                     PGPAYAQEYIDANLFAELDEYSDQYKW"
BASE COUNT          515 a          791 c          847 g          492 t
ORIGIN      
        1 taggctgacg atcgtctcac ggctaattac aaggagagac ctatggagta tcgtcagcta
       61 gggcactccg gattgtttgt ctccgcgatg accctcggca ccatgacgtt cggcggacgt
      121 ggcgactttg gcgttgtcgg ctctaccgac gtcgctacgg cgacccgcca ggtagacatg
      181 tgtctcgatg cgggcgtcaa ccttttcgac acggcggaca tgtactcaga cggcgagtca
      241 gaggagatcc tggggcaggc aatctccggg cggcgcgaca ggctgcttct ggccacgaag
      301 gcgcgcatgc ctgtggggga ggggccgaac gacgccggac tctccaggca ccacctgatc
      361 cggcagtgcg aggcgagcct caggcgcctc ggtacggact acatcgacct ctaccaggcg
      421 cacgagtggg acggcgtgac ccctctggag gagactcttg acgcgctcga caccctggtg
      481 cgttccggca aggtgcgtta cgtcggctgc tcgaaccact ccggctggca gctcatgaag
      541 gctctcggcg tctccgagcg tctcgggctg catcgttacg tgagccagca gatctactac
      601 tccctcgaag cacgcgacgc cgagtacgag ctggtgcctc ttgctatgga ccaggattgc
      661 tcgattctcg tctggagccc gcttgcaggc ggcctgctca cgggaaagta caggcgcgac
      721 caggatgcct cagagggacg ccacgtgagc ggctgggacg agccaccggt acgcgaccct
      781 gaaaaactcc acgacaccgt agacaccctc gtggagatcg ccgaatctca cggtgtctcg
      841 gctgcccagg tggccctcgc ctggctgctc ggccgccccg gtgtcgcctc ggtcgtggtc
      901 ggggcgcgca cggaagaaca actcgcggac aacctggccg ccgccgaact gcgtctctcc
      961 gaagaggagc gccggcgcct ggacgaggtg agcgccccgc ctctcctcta tccctactgg
     1021 caccaggcca ggacggcctc ggaccgtctc agccccgcgg acctgacgct cctcggcccg
     1081 cacatcgccg acgcctagat atgtgtcttt ctatcgctca acctgagggc tggttcgcgc
     1141 ggtaggagcc gggggtctgg ccagagagtc tcttgaacgt cttcgcgaag tggtggacgt
     1201 cggagaagcc gcactggccg gagatctcgc gcatcgtcag gccggtatgt tggagcaggt
     1261 cctgggcgcg ttgaaccctt aaatgcagca ggaactggtg cggcggtctg ccgaagcggt
     1321 cgcggaagac ctgcgagaaa cgggatgccg agagccctgc gcgttcggcc atgtccgaga
     1381 ccgaaagtgc ttcagagagg tgcagcgaca tgaaagaggt gatccagtta aggaactcgg
     1441 gttggtcgag agaatcggcc cgcctgcgag agtaactctg gagcacggac agtaccagtc
     1501 cggtcgcgag atagtggctc tctagctgac caaggaggtc ccgcctctgc cagattccga
     1561 tggtcttgag catggtatcc ctgaactccg cggggttttg aggcacgaac ctggtgggga
     1621 cccggatccc ggtctcctca aagagcctcg gctgcatcag ctccccaaga ccagaaacgt
     1681 ccaccctccc ggaccggaca acgaagctgt gctctcgcgc ctgattgtag aaaacgtcga
     1741 ggtgtacgta tggcgtaatg gtgttggctg gtccctccag agtatgaagc tcgcctggtt
     1801 gtatcaggca aaagtctccc tcgctaaacc ggtgcccgcg cccttcgact cgggctatca
     1861 tctctccctc ctgcacgtac atcaacaggt aatccaggag gcgccgcggg gccgtgtacc
     1921 acgcagaccg aacggggaag ctggcgtcac gaacgtatgg aacgatgggt ctgcgcatct
     1981 tcgccatcct gctatccgcc tcgacttcga tcggctccac agtcgttttt tacaacaatt
     2041 tagtgcgtgc aagcaatgat tcaagctctc gatgagcgta atatgtctgg agaaacaggt
     2101 gggaatagag gaaacgctcg cagaagggcg taaatgtacg aggtgaaaat caacaacgct
     2161 gttgtccggc gacccgcgac tcctcgcgtc cgcgttgagg atttcttccc gggtcgtcac
     2221 tgcttcaatt agtgtcgttg tccgaaaggg agttaagcat gtacggaagg gtatcgcgca
     2281 agcagtttct ggtaaggggg ggcaaggctg cgctcggtgc cggactggct ggttcgctgc
     2341 tggccgcgtg tggcggaggc ggaggcggga gcggccaggt cacgtactgg agcaacctgg
     2401 aagggtccgg gcctcaggat tacttcaaga agaacattga gaagcccttc gagaagtctc
     2461 aggacggcac tgacctcaaa gttacgttcc agccgcccga tgagatggac cggctcgtcc
     2521 gcacggcgtt gcagggcggc gaggggcccg acatcatcat gacgcctggt cctgcctacg
     2581 cgcaggagta catcgacgcg aacctattcg ctgagctcga cgagtactcg gaccagtaca
     2641 agtgg
//
LOCUS       DATAIR010000745         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_356981, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000745 DATAIR010000000
VERSION     DATAIR010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356981"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VHM69_17755"
     CDS             <1..495
                     /locus_tag="VHM69_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX2742290.1"
                     /translation="FGIAVLRSKRLSLAAENILSKLPLVNRFAPHLGDFHGASNELLG
                     TGPLVVGTAISFVAWGLECLGVYMCAVGLGADRPFLLIVFVFATSSLLGVVSMLPGGI
                     GAVEAGLYGQFVTVANLSTGMAGALTLVIRLATLWFATLLGICGLLLVRRLLGGAPLE
                     PEKP"
     gene            586..921
                     /locus_tag="VHM69_17760"
     CDS             586..921
                     /locus_tag="VHM69_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742291.1"
                     /translation="MAEASNMKVEVLYFDGCPGYETAVKKLEDVLFDKGLEAEVELVS
                     VGTDEEAELLRFPGSPTIRVDGHDLFPIAERSSYALGCRTYITDEGLRSSPTAPMLRD
                     ALEKLSARS"
     gene            973..1386
                     /locus_tag="VHM69_17765"
     CDS             973..1386
                     /locus_tag="VHM69_17765"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2742292.1"
                     /translation="MTEENKAIARKVYEIISTGEFDRAAEIVDTEAPDNELLPGDPPA
                     RLIETFKATFSEARDAFPDLSVAVEDVMAEGDRVAARVTMRGTHRGEFQGIAPTGKRV
                     EVKAIDMFRISEGRIVEHWGHADDPSRYLRAPHCP"
     gene            complement(1408..>2137)
                     /locus_tag="VHM69_17770"
     CDS             complement(1408..>2137)
                     /locus_tag="VHM69_17770"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2742293.1"
                     /translation="AVLKLQMPPYASIESEVRTLIAAEGRGYVRLLGHDGERNAMLQV
                     RLGHSLRESGLPVPAQIRVLCATLRCAWDVPAPADLPSGAEKARWLSEFISATWNELN
                     EPCSRRVIEHSLAFAEVREAAFDPESSVLVHGDAHSANALRDPRHTPPRYKLVDPDGL
                     LAEPAYDLAIPMREWSTELLAGDAARLGRERCAYLSHLTGVDPRAIWEWGFVERVSTG
                     LLATKVGADEMGREMLSVAEVWAS"
BASE COUNT          393 a          663 c          694 g          387 t
ORIGIN      
        1 ttcggtatcg ccgtcctccg ctctaaacgc ctctcgctcg cggcggaaaa catcctctcg
       61 aagctgccgc tcgtgaaccg cttcgccccg cacctcgggg acttccacgg ggcctcgaac
      121 gagctcctcg ggacgggacc gctcgtcgtg ggaacagcga tctccttcgt cgcctggggc
      181 ctggagtgcc tgggggtgta catgtgcgcc gtggggctcg gcgcggaccg tcccttcctc
      241 cttatcgtct tcgtgttcgc gaccagctct cttctcggag tcgtgagcat gctgccggga
      301 gggatcggag cggtcgaggc aggactctac ggccagttcg tcaccgtcgc aaacctctcg
      361 acaggaatgg ccggcgcact gaccctcgtg atacggctcg caaccctctg gttcgccact
      421 cttctcggta tctgcggatt gctcctcgtt agacgcctgc tcggcggcgc gcccctggag
      481 ccagagaaac catagcatcc taaacgctac cacaccccgt atacatacca tcgtagttga
      541 aagcataaga acaattagat ataacgcggg gcagaggtcg aagcgatggc ggaggcatcg
      601 aacatgaagg tcgaggtact gtacttcgac ggctgccccg gctacgagac ggcggtgaag
      661 aagctcgagg acgtcctctt cgataaagga ttggaggccg aggtcgagtt ggtttcggtc
      721 ggcaccgacg aggaggccga gttgctccgg ttccctggca gtccgacgat ccgtgtggac
      781 ggccatgacc tgttccctat agccgagcgg tcttcctacg cgctcgggtg caggacgtac
      841 atcaccgatg aaggtctcag gagctcaccc actgcgccga tgcttcgcga cgctctggag
      901 aaactctcgg cgagatccta acgtatctct tgtagtaacc ggtgcaagtt ggctgcgaga
      961 aggagacgaa tcgtgacgga agagaacaaa gccatcgcac ggaaggtata cgagatcatc
     1021 tcgacggggg aattcgaccg ggcggcggag atcgtggaca ccgaagcccc tgacaacgag
     1081 cttcttcccg gcgatccacc cgcaaggctc atcgagacct tcaaggccac cttctcggag
     1141 gccagggatg cttttccaga cctcagcgtc gcggtcgagg acgtaatggc tgaaggagac
     1201 agggtggcgg cgcgtgtcac gatgcgcggc acgcatcggg gagagttcca ggggatcgca
     1261 ccgacgggga agcgggtgga ggtaaaggcc atcgatatgt tccgcatctc tgagggcagg
     1321 atcgttgagc actggggcca cgcagacgat ccgagcaggt atctgcgcgc ccctcactgt
     1381 ccctgagtga atgacgtgtg acctgactca cgacgcccag acctcggcga cggagagcat
     1441 ctccctcccc atctcgtctg cgcccacctt cgtcgccagg agcccggtgg acacccgttc
     1501 gacgaagccc cactcccaga ttgccctggg atccacgccg gtcaggtggc tgaggtaagc
     1561 gcaccgctcg cggccgagtc gcgccgcgtc gccggcgagg agttccgtgc tccactctct
     1621 catggggatg gcgaggtcat atgccggttc ggccagcaag ccgtcggggt ccacgagttt
     1681 gtaccgtggc ggcgtgtgac gggggtctcg cagcgcgttg gcgctgtggg cgtcgccgtg
     1741 aaccagtacg gagctttccg ggtcgaatgc tgcctcgcgg acctcggcga aggcgaggga
     1801 atgctcgatc acacgtcgag aacacggctc gttcagctca ttccaggtcg ccgagatgaa
     1861 ctcggagagc cagcgggcct tctcggcgcc tgaaggcaag tccgctgggg caggcacgtc
     1921 ccaggcgcac ctgagcgtcg cacaaaggac cctgatctgg gccgggacgg gcagcccgga
     1981 ttcgcggagc gagtgtccga gccgcacttg caacatggcg ttgcgttcac cgtcgtgacc
     2041 gagcagccgc acgtaaccgc gcccttctgc ggcgatgagg gtccggactt cgctctcgat
     2101 cgacgcgtat ggcggcatct ggagcttgag gacggcg
//
LOCUS       DATAIR010000746         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_165933, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000746 DATAIR010000000
VERSION     DATAIR010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165933"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1116
                     /locus_tag="VHM69_17775"
     CDS             487..1116
                     /locus_tag="VHM69_17775"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX2742294.1"
                     /translation="MNLQIVPLAITMMAGPQIMSAIIFITARKPLKLSASFIAGAALG
                     CTVGLAITFTLATLLGNSVSLGDPSDSSSTGHIIQYLLVALLVFLAIKSFVRRETSEP
                     PRWLGTLQNARPRTAFTTGVLLLSVFPSDFIVLVTVGVNLVQNDASFVAAVPFLILTV
                     FIAALPALFYVLFHRRARQVMPKVRTWMNTNSWLVNIIVYVIFIWLILG"
     gene            1335..2861
                     /locus_tag="VHM69_17780"
     CDS             1335..2861
                     /locus_tag="VHM69_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX2742295.1"
                     /translation="MSQDRDLRHEDRTIPEYAEARGSRLDRILARTSDAPLRVGNRLV
                     LLKNGPDTYEDWLQAISKAKHWIHLDNYIFENDEIGNRFADALSAKAEEGVAVRVLHD
                     WFGCLDVPRSFWNGLREAGVEVRAVNPPASGPPFGAIRRDHRKLLAVDGEYASTGGVC
                     IADGWMVTSPESGLPYRDTAVSLRGPAVADIDRAFAGVWDETATELPESERPQAKDIQ
                     QAGEEAVRVVIQEPRKMRTLRMLELLTAGVEERLWITDPYFLSMPILTQSLMATARDG
                     VDVRVLTPSTNDIAWIGRASRAGYRQFLEEGVRIFEYGGPMIHAKTIVADGWWSKVGS
                     TNLNFSSLFANWEIDLVAEDTAFAAQMEALFEDDLSNAREVRLEGSGQHRRVRPDRKV
                     DNSDRGARSGVVGSGTGSGATVSRVGNTLLQKSGSPLQTHEHAFAAAASGALLGLSMI
                     GARFPRLVAWPLMAIGAFFGGLGLVRAVRSAVSDRAPDSDLDQTDSGSDGPVRSGGPL
                     "
     gene            2963..3154
                     /locus_tag="VHM69_17785"
     CDS             2963..3154
                     /locus_tag="VHM69_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742296.1"
                     /translation="MTQTTQSAKQEEETDLFLNRLREDLRMRLIPGEPTPRQRTALTR
                     VEAALVAYLSYFYPQDPGQ"
BASE COUNT          685 a         1011 c         1050 g          598 t
ORIGIN      
        1 ccagccacat tccttgagca gtcttcgcac ttcgcccagg gtgagtgcct gctagcaggt
       61 aagatggtgc ggaatccaac gttcgtggct tttgaggggc gcttctcgga agagggggga
      121 accgacgtgc cttcctcatg ggcgtccgtc gacgggaacc gggtgtcaca aagtccagac
      181 ggcggaggat ccctcgcatc acgaagcggt ggcccgctga ggtgaacgcg acggtctacc
      241 acgcggcatc ggaaggtcgt ggagattcac ggagatccgg acaacaaggc ggccgacacc
      301 cggaggatga aagatgtctg aagcaacagc ccgatcgagc cccgactctt ctgcggatgc
      361 gctggcattg ctccgcgctc ggcggttcat catggttacg acaacatcga tggaaaggtt
      421 ggacgctgac cttgctgagt ccttaattcc gtgtgacaaa gcgaccgtgc accgagagga
      481 gagagtttga acctgcagat cgtaccgctc gccatcacga tgatggcagg accacagatc
      541 atgtcggcga tcatcttcat cacagcccgc aagcccctta agctctctgc ctccttcatc
      601 gctggcgcgg cattaggctg tacggtgggt ctcgctatca cctttaccct cgccaccttg
      661 ctaggcaaca gcgtctccct tggcgaccct tccgatagca gttcaacagg ccacataatc
      721 cagtacctgc tagtcgctct tctggtgttc cttgccatca agagcttcgt tcgccgcgag
      781 accagcgaac ccccaaggtg gcttggtaca ctgcaaaacg ccaggccaag gactgccttc
      841 accacaggag tgcttctgct ctcggtgttc ccttcggact tcatcgttct tgtgactgta
      901 ggggtaaacc tggtgcagaa cgacgcctcg tttgtggccg ctgtgccgtt cctcatcctg
      961 actgtattca tagcggcgct tcccgcgctg ttctacgtgc tctttcacag gcgagcgcgg
     1021 caggtaatgc ctaaggtaag aacctggatg aacacaaaca gctggctagt gaacatcatc
     1081 gtctacgtca tattcatctg gctcatcctg gggtgatgaa aaacgggaaa ggagaacagc
     1141 tgtgacgctg caaatgaagg cagagggtcg ggatctcaac gaaatccggc tctccgtcaa
     1201 tcccctctac gcccgcggga actgcgcacg tatgctcgca aatacgccag acgtcgccag
     1261 atctacgagc gcgccccctg ctggaggagc gttcgaaggg cgatgatgct acagcgatag
     1321 aaggaggaca tatcttgtca caagatcgag accttcggca cgaggaccgt accattccgg
     1381 aatacgcgga ggcacgtggc agtcgcttag accggatcct ggcgcgcact agcgacgcac
     1441 cgctgcgcgt gggaaaccgg ctcgtgctgc tcaagaacgg ccccgacacc tacgaagact
     1501 ggcttcaagc catctccaag gcgaagcatt ggatccacct cgacaactat atcttcgaga
     1561 acgacgagat cggaaacagg ttcgccgacg cgttgtccgc gaaggcggaa gagggcgtag
     1621 cggtgcgggt gcttcacgat tggttcggat gcctggatgt gccgcgctcc ttctggaacg
     1681 gcctccgaga ggctggtgtg gaggtcaggg cggtcaaccc gccagcgtcg ggcccgcctt
     1741 tcggggcgat ccgacgcgac caccgtaagt tgctggccgt ggacggtgag tatgcctcga
     1801 caggcggcgt gtgcatcgcc gacggctgga tggtgacctc tcccgagtcg gggctcccat
     1861 acagggacac cgctgttagc ctgagggggc cggccgtcgc cgacatagat cgcgcttttg
     1921 ccggcgtgtg ggacgagacg gcaacggagt tgcccgagtc tgagcgcccc caggcgaagg
     1981 acatccaaca ggccggggag gaagctgtgc gcgtggtcat ccaggagccg cgcaagatga
     2041 gaaccctcag gatgctggag ttgctcaccg ccggcgtaga ggagcggctc tggatcaccg
     2101 atccgtattt cctctcaatg ccgatcctca cccagtcgtt gatggcgacg gcgcgcgacg
     2161 gcgttgacgt tagggttctc acgccgtcta ctaacgacat agcctggata ggtagagcct
     2221 cacgcgccgg ctaccggcag ttcctggagg agggggtgcg catcttcgag tacggcggcc
     2281 cgatgatcca tgccaagacc attgtggccg acggatggtg gtcgaaggtc ggatcgacta
     2341 acctcaactt cagcagcctg tttgccaact gggagataga ccttgtggcc gaggacacag
     2401 cgttcgccgc ccagatggaa gcgttgttcg aggacgacct ctccaacgct cgggaagtcc
     2461 gcctggaagg ctctgggcaa caccgaaggg tgcgccccga ccgcaaggtg gacaatagcg
     2521 accgcggggc gcgcagcggg gtcgtcggca gcggcacggg gtcgggggcc accgtctcca
     2581 gggtgggcaa caccctcctc cagaagagcg gctctccgct tcaaacccac gagcatgcct
     2641 tcgccgccgc agccagcggt gccctgctcg gcttgtcgat gatcggtgca cgctttccac
     2701 gcctcgtcgc atggccgctg atggccatag gcgccttttt cgggggcttg ggcctggtac
     2761 gcgccgttcg ctcggcggtc tccgaccggg cgccggatag cgatttggat cagacagact
     2821 cgggtagcga cggccccgtc cgcagcggag gacctctcta gcagccgacc gatgccgcgg
     2881 accttcgccg caataggaga ccagaagaac tgacgagatg aagatacacg aagaacggac
     2941 aacaacgacc tcgggaggag cggtgaccca gacaacccaa tcagcgaagc aggaggagga
     3001 aaccgacctg ttcttgaacc gtctgcgcga ggacctgcgc atgagactga ttccagggga
     3061 gccaaccccg cggcagcgca cagccctgac acgggtcgaa gcggccctgg tggcctacct
     3121 gagctacttc tatcctcagg atcctggcca atgaggcggt ggtcgtccca ctaaggtaga
     3181 cgacgcgcac agcctggcaa ccgaaaagga gggcgatgaa agcccatcga gacccacatg
     3241 gcgcagcgga cacgagagcg cctatcgtgg cggcagatcc tcggggaaat actgcctcga
     3301 gttatcagca gcggcgcgag cgtgggcgcg ccgcgcgcca gaga
//
LOCUS       DATAIR010000747         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_73260, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000747 DATAIR010000000
VERSION     DATAIR010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73260"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VHM69_17790"
     CDS             <1..434
                     /locus_tag="VHM69_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2742297.1"
                     /translation="LHVDTRGVELMDYYFADEALGVLVSRVAGGEYGEKYLILVEEDR
                     DMLENIEASLKQRSLAMMRVDEVGGEPEIVGSIPDHLSDTLQTIMDAGSITNADLAKK
                     LGLNHTAMNNRATRLVKLGLIHRKIETAAPGGRQYTYERIA"
     gene            508..1110
                     /locus_tag="VHM69_17795"
     CDS             508..1110
                     /locus_tag="VHM69_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742298.1"
                     /translation="MREEYMIERQGKRFVLYAGLLEEAHERGLRSIETELLQVPGVEN
                     GDVAIARAVVRTEDGKFSGIGDASPANVNRAIAPHVIRMAETRAKARALRDAINVGVT
                     AFEELGGDDELAVPQGAAKAPARTVASGTSTKEEAQAKEEGRGELPATRKQLNYLEAL
                     ISDVVEDGISKFEDMVGKQVSELTREEASEWIGRLSGRAA"
     gene            1110..1310
                     /locus_tag="VHM69_17800"
     CDS             1110..1310
                     /locus_tag="VHM69_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742299.1"
                     /translation="MRLWIATLGDETLYEADLPGCCQRSAHEANSTFECPTCGAIWDE
                     PQPMWAEEDAFMESPRERKGAA"
     gene            1307..1582
                     /locus_tag="VHM69_17805"
     CDS             1307..1582
                     /locus_tag="VHM69_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742300.1"
                     /translation="MRKKGALRADTTLAPAGEVEREIYVQALPNCCLRAFKTAALANA
                     GDRRRLSLECGCGRNWWITSSLDGRVLERFVTHGGPRVGSGHGPSAA"
     gene            1632..>2101
                     /locus_tag="VHM69_17810"
     CDS             1632..>2101
                     /locus_tag="VHM69_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX2742301.1"
                     /translation="MEGARDTLTQYMGRVRGGRLLDAGEEKDLARRARGGDMRARQKL
                     IESNLRLVISIAKKYRGRGVLFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAVADHARTVRLPAHVVDAIYRLRRTENALSLELGRDATEEEVIERLGVKP"
BASE COUNT          426 a          581 c          771 g          323 t
ORIGIN      
        1 tgctccacgt ggacacgcgg ggcgtcgagc tgatggacta ctatttcgcc gacgaggcgc
       61 tcggggttct tgtatcgcgg gtcgctggcg gggagtacgg ggagaagtat ctgatcctgg
      121 tcgaggaaga ccgggatatg ctggagaaca tcgaggcttc gctcaagcag cggtcgctcg
      181 cgatgatgcg ggtggacgag gtcggcgggg agccggagat cgtcgggagc atcccggatc
      241 atctctccga tacgttacag accatcatgg acgccggttc tatcaccaac gcggatctcg
      301 ccaagaagct cggcctcaac cacacggcga tgaacaaccg cgcgaccagg ctcgtgaagc
      361 tcggcctgat ccaccgcaag atagagaccg ccgccccagg cggcaggcag tatacgtacg
      421 agaggatcgc ctgatgtcta tttttatctt tagtctcact aatatcagtg aagatagcgg
      481 gaacagtgtg gcgaaggagg ttcggtgatg agggaagagt acatgatcga gcgtcagggc
      541 aagcgtttcg tgctttacgc gggcttgctg gaagaggcgc acgagagggg tttgaggagc
      601 atcgagaccg agcttcttca ggttcctggc gtggagaacg gcgacgtcgc gatagcgcgg
      661 gccgttgtca ggaccgagga cggcaagttc agcggcatcg gggacgcgag cccggcgaac
      721 gtgaaccggg cgatcgcacc tcacgtcatc cgcatggccg agacgcgggc gaaggccagg
      781 gcattgcgcg acgccatcaa cgtgggcgtc acggctttcg aggagcttgg tggcgacgat
      841 gagcttgcgg ttccgcaggg cgcagccaag gcgccggcca gaactgtggc cagcggtacg
      901 agtacgaagg aggaagccca ggcgaaggaa gagggcaggg gcgagttgcc cgcgacgcgc
      961 aagcagctca actacctgga ggctttgatc tcggacgttg tcgaggacgg catctccaag
     1021 ttcgaggaca tggtcggcaa gcaggtttcc gaactcacgc gcgaagaggc cagcgagtgg
     1081 atcgggcgtc tctcagggag ggccgcgtag tgcgactttg gatcgccacc ctcggcgacg
     1141 agactctcta cgaggcggac ctcccgggct gctgccagcg cagcgcccac gaggcgaata
     1201 gcactttcga gtgccctacc tgcggggcaa tctgggacga gccccagccc atgtgggccg
     1261 aagaggacgc cttcatggag agcccacggg agcgaaaggg cgcggcatga ggaagaaagg
     1321 cgcgcttcgg gcggacacga cgctcgcacc cgcgggcgag gtggagcggg agatctacgt
     1381 ccaggcgctc cccaattgct gcctgcgggc gttcaagacg gcggccctgg cgaatgcggg
     1441 ggacaggcgg cggctctctc tggagtgcgg gtgcgggagg aactggtgga tcacctctag
     1501 cctggacggg cgggtgctgg aaaggttcgt gacgcacggt ggtcctcggg ttgggagcgg
     1561 ccacgggccg tcggcggcgt aggggaaacc ggaagaagga ttttcggaag atcgagagga
     1621 aggggaaccg gatggaaggc gcacgagaca cactcacaca gtatatgggc agagtgcgag
     1681 gagggaggct gctcgacgca ggcgaggaga aggatctcgc caggcgcgcc cgcgggggag
     1741 acatgagggc ccgtcagaag ctcatcgagt ccaatctgcg gcttgtgatc tccatcgcca
     1801 agaaatacag ggggcgcggc gtgctcttcg aggacctcat ccaggagggg aacgcgggcc
     1861 tgatccgggc cgtcgagaag ttcgaccccg agatgggcaa ccgcttctcg acgtacgcga
     1921 cgtggtggat caggcaggct gtgacccgcg ccgtcgcaga ccatgcccgc accgtccggc
     1981 tgccggcgca cgtcgttgac gccatctacc gcctgcgccg caccgagaac gcattgagtc
     2041 tggagctcgg ccgcgatgcg accgaggagg aggtcatcga gaggctcggc gtgaaaccag
     2101 a
//
LOCUS       DATAIR010000748         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_358066, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000748 DATAIR010000000
VERSION     DATAIR010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358066"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VHM69_17815"
     CDS             complement(<1..297)
                     /locus_tag="VHM69_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742302.1"
                     /translation="MRRSVLFLSIMATVLLMAAGAVLAETLACNGGTCTGSNRADTMY
                     GSPRHDAIYSLKGGDRVRGNEGADLLNGDGGGDILAGGMGDDTVNGSDGNDVVIG"
     gene            complement(424..1425)
                     /locus_tag="VHM69_17820"
     CDS             complement(424..1425)
                     /locus_tag="VHM69_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2742303.1"
                     /translation="MTSLIVLAVLAVVVFLVAARSIRIIPQSRVAIIQRLGRYHRTAQ
                     SGLTLVVPVVDKMLPKTDLREQVIAFQPQAVITNDNVGMQISTVVYYQVVDARASEYE
                     VANFHLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDEVTEKWGIRVTRVE
                     LKEIIPPRDIQQAMEKQMQAERTRRAAILTAEGDKRSAILKAEGEKESAVLRAEGEQR
                     SAVLRAEGEAEAYRNVQAAQIEMTGKLFERLESSDLSPESLRYLYLKALPEMAKGPAS
                     KLFVVPSEIQDLAGTIGALAGGASLAGEDDAKAEQRRLDAAGRPPNGELLTRRENPS"
     gene            complement(1422..>1751)
                     /locus_tag="VHM69_17825"
     CDS             complement(1422..>1751)
                     /locus_tag="VHM69_17825"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2742304.1"
                     /translation="VLGFGVTVQAVGFLLASVLSMAVLRPAVLNRLSLRGGEGYRGHR
                     GITGESAVVTEEIEAGGKGMVRVGTGEFWTARSLHPDERIARGTRVRVLDTDGLTALV
                     ETKEEKS"
BASE COUNT          285 a          593 c          509 g          364 t
ORIGIN      
        1 gccgataacg acgtcgttgc cgtcggagcc gttgaccgta tcgtcaccca tgccgcctgc
       61 cagaatgtcc ccgccgccat cgccgttcag gaggtcggcg ccctcgttgc cacgcacccg
      121 atctccaccc ttcaggctgt agatggcgtc gtgcctgggg ctgccgtaca tggtgtctgc
      181 acggttgctg cctgtgcaag tgcccccgtt gcatgcgagc gtttccgcca ggacggcccc
      241 cgccgccatc agtagtactg tcgccatgat cgacaagaac agaaccgatc tcctcacgcc
      301 actcccttct ctccatgagg tgtgtacaac tcctgttacg tcatccgcgc agcatacagg
      361 ccgagacgcg catgcgcatc cccggagaag tatttcgcgc ctcaggcgcg gaggctcatc
      421 gcactacgac gggttctcgc gcctggtcag aagctcgccg ttcggaggtc tgcccgcggc
      481 atcgagacgg cgttgttcgg ccttagcatc gtcctcgccg gcgagactcg cgccaccggc
      541 aagggcgccg atcgtccctg ccaggtcctg tatctccgag gggacgacga agagcttgct
      601 cgctggtccc ttcgccatct cggggagcgc tttcaggtag aggtagcgga gcgattcggg
      661 cgaaagatcc gagctttcga gccgctcgaa gagcttgccc gtcatctcga tctgggccgc
      721 ctggacgttg cggtaggctt cggcctcgcc ctcggcacgc aggacggcgg acctctgttc
      781 gccctcggcg cgcagtacgg cggattcctt ctcgccctcg gctttcagaa tcgcggatcg
      841 cttgtcgcct tccgctgtca ggatcgcggc gcgacgggtc cgttcggcct gcatctgctt
      901 ctccatagcc tgctggatgt ccctgggggg gatgatctcc ttcagttcca ccctcgtcac
      961 cctgatgccc cacttctcgg tcacctcgtc gaggacagtg cggagcttcg cgttgatctc
     1021 gtcgcgggag gtcagggtct tgtccaggat gaggttgccg atcacgttcc gtaaagttgt
     1081 ctgagtgatc tgctccagcg caaggtggaa gttggcgacc tcgtactcag aagctctggc
     1141 gtcaacgacc tggtagtaga cgaccgtcga gatttgcatc ccaacgttgt cgttggtgat
     1201 gacggcctgc ggctggaacg cgatgacctg ctccctgagg tcggtctttg gcaacatctt
     1261 gtctaccaca ggtacgacca aagtgagccc gctctgggct gtgcgatggt agcgtcccag
     1321 ccgctgtatg atcgccaccc ggctctgggg gatgatcctg atgctcctcg ccgccaccag
     1381 gaataccact acagccagta ctgccaacac aatgaggctg gtcacgactt ttcctccttc
     1441 gtctccacaa gggcggtaag cccgtcggtg tcgagcaccc tcactctggt gcctctcgca
     1501 attctttcgt cgggatggag cgagcgggcg gtccagaact caccggttcc cacgcgaacc
     1561 atccctttcc caccagcctc tatctcctcc gtaaccacgg cgctctcgcc cgtgatccct
     1621 cggtgtcccc tgtacccctc tccacccctg agcgagagcc ggttcaagac agccgggcga
     1681 aggaccgcca tgctgaggac cgatgccagg aggaagccca cagcctggac tgtcacgccg
     1741 aatccaagca c
//
LOCUS       DATAIR010000749         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_220402, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000749 DATAIR010000000
VERSION     DATAIR010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220402"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..498
                     /gene="nrdR"
                     /locus_tag="VHM69_17830"
     CDS             25..498
                     /gene="nrdR"
                     /locus_tag="VHM69_17830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX2742305.1"
                     /translation="MQCPYCDFEDTKVIDSRLSETKDAIRRRRECLSCGKRFTTYERR
                     EPLRLMVIKRNGEREPFEREKLQAGLVKACAKQRISEEQIEVMVDEVEAELRDRRRHE
                     VTSRRLGDMVLLRLRRLDMVAYMRFASVYRHYTDVDQFRSELVRLAGSGVGNATR"
     gene            618..>1731
                     /locus_tag="VHM69_17835"
     CDS             618..>1731
                     /locus_tag="VHM69_17835"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX2742306.1"
                     /translation="MSEEVNYNRLSEIDLTDNAVAVLKKRYLKKNEQGVAIEEPMDMF
                     RRVASNIAEGEFRFKDGDEAQALYEESYERFLGMMTTRKFMPNSPTLMNAGRELQQLS
                     ACFVLPVEDSIDGIYDTLKHQAIIHKSGGGTGFGFSRLRPKNDLVQSTMGVSSGPVSF
                     MAIYDASTDKIKQGGTRRGANMGILRVDHPDVEEFIDCKRENDQINNFNISVAITDAF
                     MQAVEDDTDFDLTNPRNGDTIRTVRARDLFRKIVEGAWLNGEPGVVFIDRINADNPTP
                     QFPIESTNPCSEAHLPPYDSCNLGSINLERFFIEGDRPGEGTVDWEDLRETVHTAIRF
                     LDNVIEQNNYPLDEIDEMSRGNRRIGLGVMGFADLLI"
BASE COUNT          376 a          460 c          577 g          318 t
ORIGIN      
        1 tgttggagta acgggggtta cgaagtgcag tgtccatact gtgatttcga agacacaaag
       61 gtcatagact cgcgtttatc ggagacgaaa gatgcgatcc ggcggcgccg ggagtgtctt
      121 tcgtgcggga agaggttcac cacgtacgag cgccgggagc ctttgaggct gatggttata
      181 aagaggaacg gggagcggga gcccttcgag cgggagaagt tgcaggctgg tctcgtgaag
      241 gcttgcgcca agcagcggat ctcggaggag cagatcgagg tgatggtgga cgaggtcgag
      301 gccgagctgc gcgataggcg tcggcatgag gtgacctcgc ggcgtctggg ggacatggta
      361 ctgttgcggc ttcgccgtct ggacatggtc gcgtacatgc gcttcgcttc cgtgtaccgt
      421 cactatacgg acgtggatca gttccgctcc gagctggtgc gcctcgccgg ttccggcgtg
      481 ggcaacgcca ccaggtaggc cgattcgggg ggtggccttg aacgcgtgcc gcaggcgtcg
      541 tcgaagacgt tgccgcacga ggtaactgaa gattttgtcg atggaacgag cagggcaagc
      601 ggtaaaataa ggggaggatg agcgaggaag tgaattacaa ccgtctgagc gagatagacc
      661 ttacggataa tgcagtcgcg gtgctcaaga agcgctacct gaagaagaac gaacagggcg
      721 tcgccatcga ggagccgatg gatatgttcc ggagggtcgc ctccaacatc gccgagggtg
      781 agttccggtt caaagatggc gatgaggcgc aggctctgta cgaggagagc tacgagcgtt
      841 tcctgggcat gatgaccacc aggaagttca tgcccaactc gccgaccctg atgaacgccg
      901 ggcgcgagct gcagcagctc tcggcttgct tcgtgctgcc cgtagaggac tccatagacg
      961 gtatctacga caccctcaag caccaggcaa tcatccacaa gagtggtggc ggcacgggct
     1021 tcgggttctc caggctgcgt ccgaagaacg atctcgtgca gtccacgatg ggcgtctcca
     1081 gcggtcctgt ctcgttcatg gccatatatg acgcttcgac ggacaagatc aagcagggcg
     1141 gaacccggcg cggggcgaac atgggcatcc tcagggttga ccatcctgac gtcgaggagt
     1201 tcatagactg caagcgggag aacgaccaga tcaacaactt caacatctcg gtcgccatta
     1261 ccgatgcctt catgcaggcc gtcgaggacg acacggactt cgacctgacc aaccccagaa
     1321 acggcgacac gatcaggact gtcagggcga gggatctttt ccgcaagatc gtcgagggcg
     1381 cgtggctgaa cggtgagccc ggcgttgtct ttatagacag gatcaacgcg gataacccga
     1441 cgccgcagtt cccgatagag agcaccaacc cgtgctcgga ggcgcacctg ccgccttatg
     1501 actcttgtaa cctcggttcg atcaaccttg agcggttctt catagaggga gatcgtcctg
     1561 gcgagggcac ggtggattgg gaggatctgc gcgagacggt acacaccgcg atccggttcc
     1621 tggacaacgt gatcgagcag aacaactacc cgctggacga gatcgacgag atgagccggg
     1681 gcaaccgccg catcgggctc ggcgtgatgg gcttcgcgga cctgctcatc a
//
LOCUS       DATAIR010000750         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_358202, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000750 DATAIR010000000
VERSION     DATAIR010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358202"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VHM69_17840"
     CDS             complement(<1..754)
                     /locus_tag="VHM69_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742307.1"
                     /translation="MILQHIISVKELSKTYASGFQALDNVNLDIRKGEIFALLGPNGA
                     GKSTLINIICGIVNPTQGKILADGHDIISEYRAARSLIGLVPQELTTDAFETVWATTS
                     FSRGLFGKPPDPGYIEKVLRELSLWDKKDSKIMTLSGGMKRRVMIAKALSHEPQILFL
                     DEPTAGVDVELRRDMWEMVRGLRETGVTIILTTHYIIEAEEMADRIGVINHGELVLVE
                     EKDELMRKLGRKQLTLQLHDPLETIPDGLDGYG"
     gene            complement(1493..2164)
                     /locus_tag="VHM69_17845"
     CDS             complement(1493..2164)
                     /locus_tag="VHM69_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742308.1"
                     /translation="MILALVVGVASTAFGANGKPFLLGKQNVASAVSTLVKRGAGPAL
                     NLKVGSGPPLAVNSSKKVANLNADELDGLDSPSFAGSGNSVYTGGKPFCSSQQVSSTP
                     ISVTKRSLVYASAHGDVSANGNAGASTLMNIQLRNGADTTTLAQTGFSSSTYSANSFD
                     IRPLVAEGVLKSDIATPSEAPFVVTPGNNYVLRLTVSLNGGTCTGSSPYLWDSSLSYI
                     LLGTP"
     gene            complement(3040..3267)
                     /locus_tag="VHM69_17850"
     CDS             complement(3040..3267)
                     /locus_tag="VHM69_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742309.1"
                     /translation="MAIEMTGGKIVNERGTVVTFRQRCEACGYVFDWNKTTIVPAYSS
                     RKVRPFTCPECGNYQEVEVRYLHKGPRQETT"
     gene            complement(3301..>3695)
                     /locus_tag="VHM69_17855"
     CDS             complement(3301..>3695)
                     /locus_tag="VHM69_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742310.1"
                     /translation="MLLATDWEGEYGLPADIIKGATAISGLFDLAPFPFTFLQPKLQL
                     TWDQVLRNSPILRLPDAAPPLLVAYGDDETDEFKRQSEAFFEAWRAKGLEGERLTLPG
                     KNHYDVIDGFLDAGSPLCSAVLGRMDLE"
BASE COUNT          705 a         1086 c         1143 g          761 t
ORIGIN      
        1 ggccgtagcc gtcgagaccg tcagggatcg tctccagcgg gtcgtgtagc tggagcgtca
       61 gttgcttgcg gcccagcttg cgcatcagct cgtccttctc ttcgaccagg acgagctcgc
      121 cgtgatttat gaccccgatg cggtcggcca tctcctcggc ctcgatgatg tagtgggtgg
      181 tcaggatgat cgtaacgccg gtctcgcgca gccctcgcac catctcccac atgtcccgcc
      241 tgagctcgac gtcgacgccc gcggtcggct cgtcgaggaa caggatctgc ggctcgtgcg
      301 agagggcctt ggcgatcatg accctgcgct tcataccgcc ggagagcgtc atgatcttgc
      361 tgtccttctt gtcccacagg gagagctccc tgagaacttt ctcgatgtag cccgggtccg
      421 gcggtttccc gaagagcccc cggctgaagc tggtcgttgc ccacacggtc tcgaacgcgt
      481 cggtcgtgag ctcctggggg acgagtccga tcaaggacct cgccgcccgg tattctgaga
      541 ttatgtcgtg gccgtcggca aggatcttgc cttgcgtcgg gttgactatc ccgcaaatga
      601 tgttgatcag ggtcgacttc cccgcgccgt tgggaccgag cagggcgaag atctctccct
      661 tgcggatgtc caggttgacg ttgtcgagcg cctggaagcc tgaggcgtat gtcttggaga
      721 gctcctttac ggagatgatg tgctgcaaga tcaagccgcc gatcccgggt cgcgcgcaag
      781 cggacactta cggctgcgct gctttcggta tgtgacgtgc cggccggctg ccccggtcgc
      841 cccgataaga aggttcacgc tgttctcctc tcgtgtgttc tgcaactgct tcgccgcgac
      901 gctcctgagc tcgcgacgtc ttcctgtaag tttatgcgga agttcccgta gtcccaagca
      961 cctcttcctc gtttcacatg gatacgcgcg cctgtattgt ctcgtggacg gattaatttc
     1021 gcgccggacc caagtacggt ttcctgacac gtactttcgt acaggaccgc atcgaaggtt
     1081 catctaccga gccacacagg aagctggaaa gctttgaagt gaggaccggc gtctccatag
     1141 ccctggaaag ggtgtggccg acatcggcgc cggatgtagc gtcggataca gaagagcgtg
     1201 cttctactcg tggctgtagg catcgaggcg ggcgaggtac tggatcgtct cctggagcgg
     1261 gcggagcgca ccggagcccc actgttcgcc gaggccgaac tttttgcatg ctacatagaa
     1321 ggccggaacc ccgtagagtc ccgacccctt aagtcacccg acggccagat aaagaaccaa
     1381 cgtgacgtga aagcctttcc gacgggtgca ttagtaaagt gcgccagggc gcgttggaaa
     1441 atacacgaga taagagccgg gacaccccgg aggataccct ggcccgtcgt cgctaaggcg
     1501 tgccgagcag gatgtagctg aggctgctgt cccataggta cggagatgag cctgtgcagg
     1561 tgccgccgtt gaggctaacg gtcagccgca gtacgtagtt attgcctgga gtcaccacga
     1621 agggagcctc cgagggggta gcgatatcac tcttgagcac accttccgcc accagcggcc
     1681 tgatatcgaa agagtttgcc gagtacgtac ttgaggagaa tccggtctgt gccagcgttg
     1741 tggtatcggc gccgttgcgt agttgtatat tcatgagtgt gctagctccg gcgttgccgt
     1801 tcgctgaaac gtccccatgc gcgctcgcgt acacgagaga tctcttggtg accgagatag
     1861 gggtcgacga cacttgctgg gagctgcaaa aaggcttgcc gccggtgtat accgaattgc
     1921 ccgaaccggc gaaactgggc gaatccagtc cgtccagttc atctgcgttt aggttggcca
     1981 cctttttgga ggagttgacg gctaagggag gtccagatcc caccttcagg ttgagggcag
     2041 gcccggctcc acgctttacc agagtgctga ccgcgctggc cacgttctgc tttccaagca
     2101 ggaagggctt gccgttggct ccaaaggccg tagaagcgac cccgacgacc agggcgagta
     2161 tcaactgcca gtcccaccag aaacacagtg gccctaccca cccacatgac tttggttacc
     2221 gcgctcctga tcatagcgct cacgtccttt cgccttatct ctgtgatcct catcctagcg
     2281 agacgccgtc taccaaccgt tacgacaagc gttacgagcg ctaaaatcca tagcttttcg
     2341 tatcgacccc ggaagatctg gctacgctac gtcccagaca aaccagaacc ggcgtcgcgc
     2401 agcccggcct gagagacgat gcgggacaga cccatccccg cgtttcaaca tcttctacga
     2461 aacgagtggg agtgggagca tctgccacac ggcatgcctg tttgcacccc atgtagagag
     2521 gcggactcga caacccggtc ggtgtgttgt gcggctagct cgcaggtgtc gggtttccga
     2581 gcaggttgtg gtccagaatt tctcgagaaa tcactctgaa ttcatcgcct catgcttcac
     2641 tccgagggcg agcccacgtt ctcaaaggag aggctaacgg acgtgaaagc tacccttgcg
     2701 gaggccgtgg gcccgcaccc aacaatgtag actgaggggc gcggcgggtc atcccccata
     2761 tggactattt tctggagaga cgcaaagggg ctggagtcct aaaggacacc cgggcccagc
     2821 ttcgccaggg tttgcctgca ctggccgcag ccgaggagga gcctggggcg ggtcagggat
     2881 gccgacgtgt ccaggagggg taggctcgct ggaggtatct acgcagatca cctgaaatcg
     2941 ggttcaaacg gtgtggtgga gctcatagca tccttgatgc ctgatgagcg caagcgggac
     3001 aaggtaccgg acccgcccac gagagctgaa gttaggagat caagtggtct cctggcgcgg
     3061 tcccttgtgg agatagcgaa cttctacctc ctggtagttt ccgcactccg gacaggtgaa
     3121 ggggcggact ttgcgggagg agtaagcggg gactatggtc gtcttgttcc agtcgaagac
     3181 gtagccgcac gcctcacacc gctgccggaa ggtcacgacc gttccgcgct cgttgacgat
     3241 cttgccgccc gtcatctcta tggccatatc gcctccgaaa ggtgttgcgt gactgtgatc
     3301 ctattctaag tccatccggc cgaggacggc ggagcacagc ggactaccgg cgtccaggaa
     3361 gccgtcgatg acgtcgtagt ggttcttgcc tgggagcgtc agtcgctctc cttcgagccc
     3421 tttggccctc cacgcctcaa agaacgcttc cgactggcgc ttgaactcgt cggtctcgtc
     3481 gtcgccgtaa gcgacgagca ggggaggagc cgcgtcaggc aggcgcagga tggggctgtt
     3541 tctgaggact tgatcccagg tcagctgcag cttcggctga aggaaggtga aagggaaggg
     3601 cgcgaggtcg aagaggccgc tgatcgcagt ggcccccttg ataatgtccg cgggcagccc
     3661 gtactcaccc tcccagtccg tcgcgagcag catgg
//
LOCUS       DATAIR010000751         7123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_266484, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000751 DATAIR010000000
VERSION     DATAIR010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266484"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..3715
                     /locus_tag="VHM69_17860"
     CDS             212..3715
                     /locus_tag="VHM69_17860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX2742311.1"
                     /translation="MFNNKGKPVRQYEPFFTDDHSFEFEVRIGVSPVLFYDPVGRVIA
                     TLHPNHTWEKVVFDPWRQETLDVSDTVLVADPKNDADVGDFFRRLPDADYLPTWHALR
                     QNGVLGPQEQDAARKAAIHSDTPTVAHLDSLGHSFFTVAQNKFKRSDAPPADPPVKEF
                     YGTRTVFDIEGNQREVIDAKDRTVMRYDYDMLGNRAHQASMEAGERWTLTDVAGKPIR
                     AWDSRGHGFRTVHDQLQRPTESFAREGVGPERLVGRTVYGETQPNPEVGNLRGQSVRA
                     FDQAGVVTSEDYDFKGNLLHSRRQVAREYKMILDWSASVTLEAKLYASSTRYDALNRP
                     TELTTPDNSVVYPTYNEANLLNAVKARLRGAAVATPFVSDIRYDAKGQREQIQHGNGA
                     RITYEYDPLTFRLLRLLTQRNATAFPTDCPQPPPVGWPGCQVQNLRYTYDPAGNVTHI
                     RDDAQQTIYFRNRRVEPSAEYTYDAIHRLIEATGREHLGQAGGQPNPPVAPDNLDSFH
                     TRLDHPGDGDAMGTYVERYIYDAVGNMLAMQHRGSDPAHPGWTRTYAYNETSKLEPER
                     ANNRLSSTTVGNTVENYRYDGTAGLHGNITSMPHLPLMRWDYHDQLRATSRQEFNVGT
                     PETTWYIYDGGGQRVRKVTERQAAAGQTPTRKQERIYLGGFEIYREFAGDGTTVTLER
                     ETLHVAAALDRVALIETRTVGNDPAPEQLIRYQYTNHLGSAVLELDDDADIISYEEYL
                     PYGSTAYQAVRGQTETPKRYRYSGKERDEENDLYHHGARYYAPWLGRWTSCDPAGLMD
                     GHNLYRYVSNNPLSFSDPSGRQKSDAVGTISTHRKHERSLYDALGNRISEDEHVIPKA
                     SQKAVTRDPTTGLSDFTERHYQNNTTVRVEKSMANEKTRGGPTSDNARSAALKQKALT
                     GTPMDVNQELLVDSLDNAKRARTATGSVITDDQLHKTALAEMGELFETHGHDTRKKVE
                     LSWERMSDAEIQKEQQSLQRASESAKKLAPEKAADTSKAVLNKADDVAEAAAKPTTKA
                     LETVAEKGGMKIATKLGTKALKVVPFVGMGASLYSMKAEAAQGNYGAAALEGVGMVPV
                     VGDVVDAARLGVAVGETASEALGIEEVATEHGMAVEGAARSLGFSQDTSRLIGATGAA
                     LSSITVAPQIALQRKIAGWFR"
     gene            3965..>7123
                     /locus_tag="VHM69_17865"
     CDS             3965..>7123
                     /locus_tag="VHM69_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX2742312.1"
                     /translation="MQLSRELRPTMTGPDVAALHQELAKLGFRIPGEESQQRRFGRGT
                     QEAVRTFQGDHGLDVTGVVNQPTADAINAAVVAAAAKKLAISGQLRQQDATPVVGALV
                     RAFEVGSAGDGNILGEAKTGLGGSYRLQCELKAGSEGTDIQVRAFEEDGTTLLAESEV
                     LRGVRTDITVNLTVGTFAKRDYVVKGHVRWEDEGWVVGHSVRAFELMQSGERQLGEDT
                     TDSQGQYEISYQYSGLKGRDLVVRVYDEQGAELVSSGRIENAHQTEVVHLTASREPEP
                     GDDFVVRGEVRGADGALLAGTTVRAFDVDLRHEEQLGEQATGEAGRYEIRYRREQFSR
                     AEKESADLRVRVLGSGGEVLVASPVIFNAQPIEEVNLMIGGGQFEGPSEYELLVADLT
                     PLVGDVPFAELTEEDVTFLAGETGEDAGRIGFLARAHLFSGETEVPAEAFYGMFRQKL
                     PTELAALLAESSDAQRQALVTAVEENIIPVSFRDRIEEILERLEDLAPGQVLDPPAPD
                     GGASVDDLLRTTLGDEGLREQFASAYAAHTGPIQEFWDGLSERPEFADKVEDLQFTVQ
                     LGGVTGNRLELVEKLQSLKEAGEISTVRDLARFDAEEWRQIVSEEGVGVPPGVPGQDD
                     DEKVQNYASAMAGVVEDAFPTSFVARRLESEVGSGVARSLAPVSSGEHLRTFFSDNED
                     SFDFAGTRLQSYLVENPDALAGIPEENRPRVQQQVETMQRLYRVAPRYEQMRVLMDDG
                     LHSAQGITLMGQSAFAIKYSAALSGEAQANQVFENAQQVTSTAMLLLADAGMPSKRLA
                     MTAVPDTTADEVEGIPDWRALFRSLDLCACEHCRSVYSPAAYLVDVLHFLGGRRLVDT
                     NSPNGDDGPVIGISFETNLLDDGTEADATARDVLFDRRPDLGKIELTCENTNVPLPYV
                     DLANEAMEEFIAPSKVHSGVGDFSATENPTGAWSYGYRASPDSDFTPYDDRGNPWGES
                     FDQWSLNDLEPHVTYNNTGSTASHATIVHPPDVLNVHPGPAGERSVVRWIAPTPGTVA
                     IEGRFEGIDTAGTSTDVAVVHNS"
BASE COUNT         1477 a         2321 c         2275 g         1050 t
ORIGIN      
        1 ctccgatctg aggccgggac agagaacgaa ggtccagcac ggcttctcct actcggacgg
       61 tttcgggcgt gagatccaga aaaaagccca ggcggaacca gggccggtgc caaggcgaga
      121 tcccgtaacc ggccagatca tcatcgtcga cggccgacca gagatgactc cgaacgatgt
      181 tagcccgcgc tgggtaggta gcggctggac ggtgttcaac aacaagggca agcccgtccg
      241 ccagtacgaa ccatttttca cggacgacca cagcttcgag ttcgaggtgc gcatcggggt
      301 cagcccggtg ctcttctacg accccgtcgg acgcgtaatc gccaccctgc acccgaacca
      361 cacctgggag aaggtggtct tcgatccgtg gcggcaggag acgttggacg tcagcgacac
      421 cgtgctggtg gccgatccca agaacgacgc cgatgtgggc gatttcttcc gccgcctgcc
      481 ggacgcggac tacctgccta catggcatgc gctgcgccag aacggtgtcc ttggtcccca
      541 agaacaagat gccgcccgca aagcggctat ccattcggat acaccgactg ttgcgcatct
      601 cgactccctt ggacactcct tcttcaccgt cgcccagaac aagttcaagc gcagtgacgc
      661 gcccccggcc gatccgcccg tcaaagagtt ctacggcacg cgcaccgtct tcgacatcga
      721 aggcaatcag cgcgaggtta tcgacgccaa ggaccgcacc gtcatgcgct acgactacga
      781 catgctcggc aatcgtgccc accaggcgag catggaggcg ggtgaacgct ggaccctgac
      841 tgatgtcgcc ggcaagccaa tccgcgcctg ggacagccgc ggtcatgggt tccgcaccgt
      901 ccacgatcag ctacagcggc ccaccgaatc gtttgcgcgt gagggagtgg ggccggagcg
      961 gctggtgggg cgcaccgtct acggcgaaac ccagcccaac ccggaggttg gaaacctacg
     1021 cgggcaatcg gttcgagcct tcgatcaggc tggcgtcgtc accagcgagg attacgattt
     1081 caagggcaac ctgctgcaca gccgacgtca agttgcccgt gagtacaaga tgatcctgga
     1141 ctggtcggcc agcgtgaccc tggaagcgaa gctctacgcc agcagcaccc gctacgatgc
     1201 gcttaaccgc ccgacggaac ttaccacgcc ggataacagc gtcgtgtacc ccacctacaa
     1261 cgaagccaac ctgttgaatg cagtgaaagc gaggctacgt ggtgccgcgg tcgccacccc
     1321 tttcgtcagc gacatccgct acgacgccaa gggccagcgc gagcagatcc aacacggcaa
     1381 cggcgccagg ataacttacg aatacgaccc gctgaccttt cgtctgctcc gcctgctcac
     1441 ccagcgcaac gccactgcct ttcccaccga ttgcccgcag ccgccaccgg tcggctggcc
     1501 cggttgtcag gtgcagaacc tacgatacac ctatgaccct gcgggcaacg ttactcatat
     1561 tcgcgacgac gcccagcaga ccatctactt ccgcaaccgg cgcgtcgagc cgagcgccga
     1621 atacacctac gacgccatcc accggctgat cgaagcgacg ggacgagagc acctggggca
     1681 ggccggtgga cagcccaatc cgccggtcgc gcccgacaac ctcgatagtt tccatacccg
     1741 cctcgaccac ccgggcgacg gcgacgcaat gggcacgtac gtcgagcgct acatctacga
     1801 tgccgtgggc aacatgctgg ccatgcaaca ccgtggcagc gacccagcgc atcccgggtg
     1861 gacgcgcacc tacgcctaca acgagaccag caagctcgaa cccgagagag cgaacaaccg
     1921 ccttagttcc accactgtgg ggaacacggt cgaaaactac cgctacgacg gcaccgccgg
     1981 gttgcacggc aacatcacga gcatgcccca cttgcccctg atgcggtggg actaccacga
     2041 ccagcttcgg gccacttctc gtcaggagtt caacgtcggc acaccggaga caacctggta
     2101 catctacgac ggtggcggac aacgcgtgcg caaagtgacc gagcgccagg ctgccgccgg
     2161 gcagacaccc acgcgcaaac aagaacgcat ttacctgggt gggttcgaga tataccgcga
     2221 gttcgccggc gacgggacca ccgtgacgct ggaacgcgag acccttcacg tcgcggcggc
     2281 tctggatcgc gtcgccctga tcgaaacgcg cacggtcggc aacgatcccg cacccgagca
     2341 gctgatccgc taccagtaca cgaaccacct cggctcggct gtactggagc tcgacgatga
     2401 tgccgatatc atctcctacg aagagtatct cccctatggc tccacggcct accaagcagt
     2461 gcgcggccag accgagactc ctaagcgcta ccgctacagc ggtaaggagc gcgacgaaga
     2521 gaacgacctc taccaccacg gcgcccgcta ctacgccccg tggctcggcc ggtggacgag
     2581 ttgtgacccg gccggactta tggacggcca caacctgtac cgatacgtta gcaataatcc
     2641 gctctccttc tctgacccta gcggacgtca gaagagcgac gctgtaggca cgatctccac
     2701 gcaccgcaag cacgaaaggt ccctttatga tgctttgggg aaccgcatct ccgaagacga
     2761 gcacgtcatt ccgaaagcta gccagaaagc cgttactcgc gaccctacga cgggcttgtc
     2821 cgacttcact gaacgccatt atcaaaacaa cacgaccgtc cgggtagaga aaagcatggc
     2881 aaacgaaaag acccgtggcg gcccgacctc cgacaacgcg cgatcggccg ccctcaaaca
     2941 gaaggcgctc actggaaccc caatggacgt caaccaagag ctcctcgttg attctctcga
     3001 caacgcgaag cgcgcgcgaa ccgcgaccgg ctccgtgatc accgatgacc agttgcacaa
     3061 gacggcgcta gccgagatgg gtgaactgtt tgagacccac gggcacgaca ctcggaagaa
     3121 ggtagagctc tcgtgggagc gcatgagcga cgccgagatc caaaaggagc aacagagcct
     3181 gcagcgggcc tcggaatcgg cgaaaaagct cgcacccgaa aaagccgccg acacgagcaa
     3241 ggcggtcctc aacaaagcgg acgatgtggc cgaggccgcc gccaaaccca ctacgaaggc
     3301 gctcgaaacc gttgcggaga aaggcggcat gaaaatcgcc acgaagctcg gaacgaaggc
     3361 gttgaaggtg gtcccattcg tcggcatggg cgccagtctc tactcgatga aggccgaggc
     3421 ggcccaggga aactacggag cggccgccct ggagggcgtc ggcatggtcc cggttgtcgg
     3481 cgatgtggtc gatgccgccc ggctcggcgt ggccgtcggg gagactgcca gcgaggcgct
     3541 gggcatcgaa gaggttgcca ccgagcacgg catggccgtc gagggtgctg ccagatcact
     3601 cgggttcagc caagacacct ctcggcttat cggggcgacc ggcgcggcgc tcagctcgat
     3661 caccgttgcc ccgcagatcg ccctccagcg aaagatcgcc ggatggttca ggtagggctg
     3721 ccggccgtaa gtactacagc ggatgcgagg aggcacatga tgctacagga atacaatctc
     3781 cgaccagaga tcctcggtga gagcgtgtgt ctcccacgca cagaaggcgc gcagatcggt
     3841 cacgaacaga tgttcggtgc tcatagcttc ccgccgagat ccgagcaacg ggtagaaaga
     3901 cagaccaccc taggaaacag gcgaactacc tctacctgct ccagcaacga aaggaagttt
     3961 gatcatgcaa cttagtcgcg agctcaggcc cactatgacc ggccccgacg tcgccgcgct
     4021 gcatcaggag ctggcaaagc tcggcttcag gatccccggg gaggagtcgc agcagcgccg
     4081 cttcgggagg ggcacgcagg aggccgtgag gacgttccag ggggatcacg gcctggatgt
     4141 gaccggggtg gtgaaccagc cgacggcgga cgcgattaac gccgctgtgg tcgccgctgc
     4201 agcgaagaag ctcgcgatca gcgggcagct acgccagcag gacgcgacac cggtagtcgg
     4261 ggccctggtg cgcgcgttcg aggtcgggtc ggccggcgac ggaaacatcc ttggcgaggc
     4321 gaagaccggt ctgggcggct cttacaggct gcagtgcgaa ctgaaggccg gatctgaggg
     4381 cacggacatc caggtgcggg ccttcgagga ggacgggacg acgcttctgg ccgagtcgga
     4441 ggtcctgcga ggcgtgcgga cggatatcac ggtgaacctc acggtgggca ctttcgccaa
     4501 gagggattac gtggtcaagg ggcacgtgcg ttgggaggac gaaggctggg tcgtcgggca
     4561 ctcggtgcgc gccttcgagc tgatgcagag cggcgaacgg cagcttggcg aggacaccac
     4621 ggattcgcag ggtcaatacg agatttccta ccagtactca gggctcaaag gccgcgacct
     4681 ggtcgtgcgc gtgtacgacg agcaaggggc tgagttggtg tcctcgggga ggatagagaa
     4741 cgctcaccag acggaggtgg tgcacctgac agcctcccgc gagcccgaac ccggcgacga
     4801 cttcgtcgta aggggagagg tccgtggcgc ggacggcgcg ctcctggccg gcacgaccgt
     4861 gcgggcgttc gacgtggacc tgcgccacga ggaacaactg ggcgagcagg cgaccggcga
     4921 ggcagggcgc tacgagatcc ggtaccggcg cgagcagttc agccgcgctg agaaggagag
     4981 cgccgacctc cgggtccgcg tactcggctc gggaggcgag gtcctcgttg cctcgcccgt
     5041 catcttcaac gcccagccca tcgaggaagt gaacctcatg atcggcggcg gccagttcga
     5101 gggaccctcg gagtacgagc tgctggtagc cgacctgacc ccgctcgttg gggacgttcc
     5161 cttcgcggag ctgaccgaag aggacgtgac cttcctagcg ggtgagacgg gcgaagacgc
     5221 cggccgcatc ggtttcctcg cccgggccca cctgttctcg ggggagacgg aagttccggc
     5281 ggaggccttc tacgggatgt tccgccagaa gctgcccacc gagctggccg ccctgctggc
     5341 cgaaagctcc gacgcccagc ggcaagcgct cgtgaccgcc gtggaggaaa acataatacc
     5401 cgtgagtttc cgggaccgta tagaggagat cctcgaacgc ctcgaagacc tggcgcccgg
     5461 acaggtcctg gacccgcccg ccccggacgg cggagcctcc gtagatgact tgctgcgcac
     5521 gacgctgggg gacgagggac tgcgagagca gttcgccagc gcctacgccg cacacaccgg
     5581 tcccatccag gagttctggg acggcttgag cgagagaccg gagttcgcgg acaaggtgga
     5641 agatctgcag ttcaccgtgc agctcggagg ggtgaccggt aaccgcctgg agctcgtgga
     5701 gaagttgcag agcctcaagg aagcaggcga gataagcacc gtgcgcgacc tcgcgcgctt
     5761 cgacgccgag gagtggcgcc agatagtctc tgaggaaggc gtcggggtcc cgcccggagt
     5821 ccccggacag gacgacgacg agaaggtcca gaactacgcc agcgccatgg ccggcgtggt
     5881 cgaggacgcg ttccccacgt cgttcgtggc ccgccggctc gagagcgagg tggggagcgg
     5941 tgtggcgcgc agcctcgcgc ccgtgagtag tggggagcac ctccgcacct tcttctcgga
     6001 caacgaggac agtttcgact tcgcgggcac caggctgcag agctacctcg tagagaaccc
     6061 cgacgcgctc gccggcatcc cggaggagaa caggccgcgg gtacagcagc aggtcgagac
     6121 catgcagcgg ctctacaggg tggcgccgcg ctacgagcag atgcgggtgc tcatggacga
     6181 cgggctgcac tccgcgcagg gcatcacgct catggggcag agcgcgttcg cgatcaagta
     6241 ctctgcggcg ctgagcgggg aggcacaggc caaccaggtg ttcgagaacg cccagcaggt
     6301 aacctcgacg gcgatgctcc tcctcgcgga cgccggcatg ccctccaagc gtctcgcgat
     6361 gacggccgtc ccggacacga cggctgacga ggtcgagggc ataccggact ggcgcgccct
     6421 cttcagatcc ctggatctct gcgcctgcga gcactgccgc tcggtgtaca gccccgcggc
     6481 ctacctggta gacgtgctcc atttcctggg cggccggcgc ctggtcgata cgaactcccc
     6541 gaacggcgac gatggtcccg tcataggcat ctcattcgag acgaatctcc tcgacgatgg
     6601 caccgaggcg gacgcgaccg cgagggacgt cctcttcgac cggcgcccgg acctcggcaa
     6661 gatcgagctc acctgcgaga acacgaacgt tccccttcct tacgtcgacc tcgccaacga
     6721 ggcgatggag gagttcatcg cgccttcgaa ggtccacagc ggcgtcggcg acttctccgc
     6781 gaccgagaac ccgaccggcg cgtggagcta cggctaccgg gcatcacccg attcggactt
     6841 caccccgtac gacgaccgcg gcaacccctg gggggagagc ttcgaccagt ggtccctgaa
     6901 cgacctcgaa ccccacgtca cgtacaacaa cacagggagc accgcttcgc acgccacgat
     6961 agtccatcct ccggacgtgc tgaacgtcca ccccggtccc gccggcgaaa ggagtgtggt
     7021 gcgctggatc gcgccgacgc ccggcacggt ggcgatcgaa ggcaggttcg aggggataga
     7081 cacggccgga accagcaccg acgtggccgt cgtccataac tcc
//
LOCUS       DATAIR010000752         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_358593, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000752 DATAIR010000000
VERSION     DATAIR010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358593"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VHM69_17870"
     CDS             <1..743
                     /locus_tag="VHM69_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2742313.1"
                     /translation="PYNDLDAVRGLFEREGEEFAAVILEPVAGNMGCVPPAEGYLEGL
                     REITSEYGTVLIFDEVMTGFRVARGGAQERYGVTPDLTCLGKIIGGGLPVGAYGGKRE
                     IMDQVAPSGPVYQAGTLSGNPLAMAAGLATLRIASQPGFYDRLEELGGLWRRGMTEAA
                     SAGSRPFSINQVGSMVSIFFTGGPVTDFGSAASSDTEAFKDFFWHMLSHGIYLAPSQY
                     EAGFISTAHSEEDLQRIFEAAGDWFEGS"
     gene            744..1163
                     /locus_tag="VHM69_17875"
     CDS             744..1163
                     /locus_tag="VHM69_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742314.1"
                     /translation="MEETARRTPEVSLKDTFLLAVAGLPGEDVRAGVVAEALSYLQSG
                     FDAHYAEGGATDDDVLVGDNAYALAVETIARLDEPRFVAVASRMIRDGAGRIAAGGAV
                     TVEMWTPHLAGLLDIISEEGTQRSEARIRDAAEELRG"
     gene            1156..1896
                     /locus_tag="VHM69_17880"
     CDS             1156..1896
                     /locus_tag="VHM69_17880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2742315.1"
                     /translation="MGKLRKTARDRLPSGAERALLVREMFDRIAGRYDLLNTVMTAGL
                     HRHRNRKVVKATGLQPGGSAIDLACGTGTLTRELAKRVGPEGYVLGVDFSREMLRAAK
                     SAPNVEYRLGDATHLGGVPSGAFDAATIAYGARNIPDLDALFSEMARTLKPGGVAVCL
                     EIARPTGKVFSIFYGLWFDNIVPRLGGLISGDASAYSYLPESVKEFVAPGELADIIGR
                     NGLQDVTWKRLAGGIITLHRGIRRSQGG"
     gene            1811..2860
                     /locus_tag="VHM69_17885"
     CDS             1811..2860
                     /locus_tag="VHM69_17885"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2742316.1"
                     /translation="MDYKTLHGSGSRAASSRSIVGSGEAKAASELHTALPEGAFETID
                     EVEGLLACVAERAPVDLIEPSLGSLTSGGKRLRPLLLVLSAQMGEPERDDLMGAATAI
                     EVLHTATLIHDDIVDRAESRRGVPTTVARYGREIAAATGDYLFAEAFSELARIGNPRL
                     VRAFAEASVGLAAGELEQYRANGATVEVEAYLEHIRKKTAGLFQAACVAGGTLGGLSL
                     VQIDALATYGQALGIAFQMSDDIMDLVGKPGLMGKGIGTDLVEGTVTLPVIFALREGD
                     TATIRRVLADPNPTPELLEAGIEAVLATDAVTRTEAWARGEIDAALEDLGLLPDCPER
                     DFLESVASEVVGRDA"
     gene            2853..>3275
                     /locus_tag="VHM69_17890"
     CDS             2853..>3275
                     /locus_tag="VHM69_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742317.1"
                     /translation="MPESRPVIFAAGTVMPVSSPHIQDGAVLVRDGRIAAVGALADVG
                     RDNPGVEIRFFPNYTIVPGAVNAHAHLGFRRKDAPEGGTFSGWLGRLIKRLPEKEAWT
                     TGAASDSAREAREAGTTYMAESSPYGECLPQLAESGMAG"
BASE COUNT          584 a         1024 c         1120 g          547 t
ORIGIN      
        1 tgccgtacaa cgacctggac gcggttcgag gcctcttcga gagagaggga gaggagttcg
       61 ccgccgtcat ccttgagcct gtcgcgggga acatgggatg cgttccaccc gcagaaggtt
      121 atctggaggg gttgagagag atcacgagcg agtacggtac cgttctcatc ttcgatgagg
      181 tcatgaccgg tttcagggtc gctcgtggcg gggcgcagga acgctacggc gtcacgccgg
      241 atctgacgtg cctcggtaag atcatcggcg gaggtctccc tgtcggggcg tacgggggga
      301 agcgggagat catggatcag gtcgcaccct cgggccccgt ctaccaggcc ggaaccctct
      361 ccggaaaccc gctcgctatg gcagcgggcc tcgcgacgct tcgcatcgct tcacaaccag
      421 gcttctacga caggctggag gagctcggcg ggctctggcg caggggaatg acagaggcag
      481 cctcagccgg ctctcgtccg ttctcaataa accaggtagg ctcgatggtg agcatctttt
      541 tcaccggtgg acccgtcacg gatttcggct ccgcagcctc gtcggacacc gaggccttca
      601 aggatttctt ctggcatatg ctctctcatg gtatctacct ggctccgagt cagtacgagg
      661 ccggcttcat ctccaccgcc cactccgagg aagaccttca gaggatcttc gaggctgccg
      721 gcgattggtt cgaggggagc taggtggagg agacggcccg ccgcacgccc gaggtctcgc
      781 tcaaggacac gtttctgctg gccgtcgcgg gacttcccgg cgaagatgtg cgggccgggg
      841 tcgtggccga ggcgctctcg tacctgcaga gcggcttcga cgcccactac gccgagggag
      901 gggcgaccga cgatgacgtg cttgtcgggg acaacgcgta cgcgctcgcg gtcgagacca
      961 tcgcgcgcct cgacgagccg cggttcgttg ccgtggcgag ccgtatgatc cgtgacggcg
     1021 ccggtcgcat cgccgccggc ggagcagtca ccgtggagat gtggacgccc cacctggcag
     1081 gtctcctcga catcatctca gaggagggca cacagaggtc cgaggcgagg atacgggacg
     1141 ctgccgagga gctgcgtggg taagctccgc aagacagctc gggacaggct gccgtcaggg
     1201 gcggagcggg cgctgctcgt gcgggagatg ttcgaccgga tcgctggccg ctacgacctc
     1261 ctgaacaccg tcatgaccgc gggactccac cgccacagga acaggaaagt cgtaaaggcc
     1321 acgggcctcc agccgggcgg atcggccatc gacctcgcct gcggcacggg taccctcacg
     1381 cgcgaacttg cgaagcgggt cgggcccgag gggtacgtgc tcggcgtcga cttctccagg
     1441 gagatgctgc gcgcggctaa gtccgcgccc aacgtcgagt acaggctcgg ggacgcgacg
     1501 cacctgggag gcgtcccatc aggcgccttc gacgccgcca ccatagccta cggggcgagg
     1561 aacatccccg acctcgacgc gctcttctcc gagatggcgc gcacgctcaa gcccggcggc
     1621 gtcgccgtgt gcctggagat agcacggccc acggggaaag tcttttccat attctacggg
     1681 ttgtggttcg ataacatcgt tccaagattg ggtggtttga tctcgggaga cgcatcagcg
     1741 tattcatatc ttcccgaatc cgtgaaagag ttcgtggcgc ccggcgaatt agctgacata
     1801 ataggacgta atggattaca agacgttaca tggaagcggc tcgcgggcgg catcatcacg
     1861 ctccatcgtg ggatcaggcg aagccaaggc ggctagcgag cttcacaccg cgctgcccga
     1921 gggcgccttc gagaccatcg acgaggtcga gggcctgctc gcctgcgtcg cggagcgcgc
     1981 gcctgttgac ctcatcgagc cgtctctcgg gtccctcacg tcggggggga agcgtctcag
     2041 gccgctgctc ctcgtactca gcgcgcagat gggggagccg gaacgggatg acctgatggg
     2101 agccgctacg gccatcgagg ttcttcacac tgcgaccctg atccacgacg acatcgttga
     2161 cagggctgaa agtaggcgcg gagtgccgac caccgtcgcc aggtacgggc gtgagatcgc
     2221 cgcggccacg ggagactacc tcttcgccga agcgttctcg gagcttgcca ggatcggcaa
     2281 ccccaggctg gttcgcgcgt tcgccgaggc ttcggtgggg cttgccgcgg gagagctgga
     2341 acagtaccgt gcgaacgggg ctaccgtcga ggtcgaggcg tatctggagc acatcaggaa
     2401 gaagaccgca gggctcttcc aggcggcgtg cgtggcaggc ggtactctag gcggactttc
     2461 gctcgtgcag atagacgcgc tcgcgaccta cggccaggcc ctcggcatcg ccttccagat
     2521 gtctgacgac ataatggacc tcgtcggcaa gcctggcctg atgggcaagg gcataggcac
     2581 ggacctcgtc gagggtaccg tgaccctgcc cgtcatcttc gccttgaggg agggcgacac
     2641 ggcgacgata cgacgcgtcc tcgccgatcc gaaccccacg cccgagctgc tcgaggccgg
     2701 aatagaggcg gtgctcgcaa cggacgccgt cacaaggacc gaggcgtggg ccaggggtga
     2761 gatcgacgcg gccctcgaag acctcggcct cctgccagac tgccccgaac gagatttcct
     2821 cgagtcggtg gcatcggagg tggtcgggag ggatgcctga aagtcgaccc gtgatcttcg
     2881 cggccggcac cgtcatgccg gtctcctctc cccatataca ggacggcgcc gttctggtca
     2941 gggacgggcg gatagcggct gtcggagcgc tggcagacgt cggacgcgat aatcctggcg
     3001 tcgagatccg gttcttcccc aactacacga tcgtccctgg cgcggtaaac gcccacgccc
     3061 acctcggctt cagacgtaag gacgcgcccg agggcggcac gttctcgggc tggctgggca
     3121 gactcataaa gcggctaccc gagaaggaag cctggacgac cggggccgcc agcgactccg
     3181 cccgcgaagc tagagaggct gggacgacgt acatggccga gtcctctccc tacggggaat
     3241 gcctgccgca gctcgccgag agcgggatgg ctggg
//
LOCUS       DATAIR010000753         6578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_220973, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000753 DATAIR010000000
VERSION     DATAIR010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220973"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VHM69_17895"
     CDS             <1..517
                     /locus_tag="VHM69_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2742318.1"
                     /translation="AARSAVDFRDDAKRRDDVRATTGFVNVSPAIVTAFNGWCEMSLD
                     QRALDPSVLASMLDAAQEATARVATEEDVSVEWEKLWRIEPIPFDDELISLAEEAVNE
                     IAGQSHRLPSGPLHDAAEMARLMPTVMLFVKSLRGLSHTKDEDTPEEDLELSVQALHR
                     LAVKTMNWAGK"
     gene            595..2118
                     /locus_tag="VHM69_17900"
     CDS             595..2118
                     /locus_tag="VHM69_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX2742319.1"
                     /translation="MEEFKDLAVNNEAELQRIKSGAMASSLYNEDLAPTGPEQRTWTT
                     YNIAALWIGMAIVITTYTLASGLMAAGMNWWQALLTISLGNVLVLIPMLLNAHAGTKY
                     GVPFPVFVRASFGVRGANFAAMARALVACGWFGIQTWIGGLALDALMKAGWGGWESVP
                     GHSFITFLVFWAIQLVIILRGIEGVKIFESLSAPLLLGGSLVLLIWGFSAGGGIANVF
                     ETSAKLQQGDAPFWALFWPSLAANVGYWITLSLNIPDFTRYAKTQRSQVLGQALGLPL
                     TMTAFSFIGIAVTAATIVVYGEAIWDPVVLVTKLAENAPWLLILAMIIIAIAQISTNM
                     AANVVSPSFDFSNLAPKYISFRTGGIITALIGIISFPWVLFNNVGAYIFTWLVGYGSL
                     LGAIGAVMIADYWIVRRRQLDLAELYKLDGRYSYSNGWNWRAIAAVFIGVIPVLPGFF
                     KAATTPGFAGVFQDPTFIESLYNYGLFFTFGVAGLAYLALSMIGGRAAEPASEPEAT"
     gene            2129..2767
                     /locus_tag="VHM69_17905"
     CDS             2129..2767
                     /locus_tag="VHM69_17905"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX2742320.1"
                     /translation="MPDASTFALFVAAALVMLIVPGPSVLYIVARSVEGGRTAGFVSV
                     LGVQTGALVHIAFATLGLSAILASSAVAFSVVKWLGAAYLIWLGLRRIFGSDEEEGDV
                     AVEPERLSRVFSQGVIVNTLNPKTALFFLAFLPQFVDPSRGAAWAQILLLGATFVILA
                     LCSDGLYALLSGTAGGWLRRKMEGADFRRGQRYVSGGVLVALGAVATAAGKD"
     gene            2778..3788
                     /locus_tag="VHM69_17910"
     CDS             2778..3788
                     /locus_tag="VHM69_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2742321.1"
                     /translation="MDFGVTLQTNPPASRVIELTRKAEDLGFGYAWTFDSHILWQEPY
                     VIHSQMLSATENIVVGTFVTNPVSRDPSVTASTFATLNGMFGNRTICGIGRGDSVVRV
                     LGRKPTTLKQLEEAMHVIKELAEGREVDYGDVKVRIPWVEDGKLDVWMAGYGPKALSL
                     IGRKADGFILQLADPVILEWTMQHVHDAAKEAGRDPKSVKICVAAPAYVGDDLAHQRD
                     QCRWFGGMVGNHVADLVSRYGEDGDVPHALTDYIKARESYDYSHHGQAGNPSTDFVPD
                     DIVDRFCVLGTVDDHVKKLNELKALGADQFNVYLMHDAMEETLYAYGEEIIPTLGAKS
                     FR"
     gene            complement(4203..4556)
                     /locus_tag="VHM69_17915"
     CDS             complement(4203..4556)
                     /locus_tag="VHM69_17915"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX2742322.1"
                     /translation="MIRLAADENLNYDIVRGVLRRNPEVDIIRVQDAGLSGAEDPAVL
                     EWAAQEGRVLLTQDVATMTRYAYERVREGKPMPGVFEVGRGMPAGLAIEEILLIAECS
                     LEGEWEGQVRYLPLR"
     gene            complement(4562..4888)
                     /locus_tag="VHM69_17920"
     CDS             complement(4562..4888)
                     /locus_tag="VHM69_17920"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX2742323.1"
                     /translation="MSLAVAPEPIPLTTDRNGVVRVTGTRVTLDTVVAAFQEGATAEE
                     LAQQYPSLRLADVYAVIGYYLRHQLEVDEYLRLRQQFAAEARLANEGRFDPAGVRARL
                     LARRAK"
     gene            complement(4951..5157)
                     /locus_tag="VHM69_17925"
     CDS             complement(4951..5157)
                     /locus_tag="VHM69_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742324.1"
                     /translation="MSLRQEERLVERRVTYALELDGKVYIVENVPARVDEETGKQYFS
                     PSTTERLQQIVLEHIEPSCSRPLQ"
     gene            5371..>6578
                     /locus_tag="VHM69_17930"
     CDS             5371..>6578
                     /locus_tag="VHM69_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2742325.1"
                     /translation="MTEPAISVQGLGYRYRGQQKPALQGIDLEVAEGEFVVVMGPSGA
                     GKSTLCVSLNGLIPHFHRGRMEGEVKVRGRSTRDGKVGEFAKEVGLVFQDFEAQLFST
                     NVALEVAFGPENFAVEHDEMLRRVKDVLGQVRLEGFEGRQPATLSGGQKQRLAIASVL
                     AIEPRILCLDEPTTDLDPAGKLGVFEIAEELKDRDDVTLIVVEHETEETLDADRIIVL
                     RDGEIIADRPAREVLRDVELLEESGVMPLQVARFFHEMGLWQAQLPLTPEEGSQEFRR
                     RGWRVNPDHHRALVAEDGQRAEGYGEPIIEIDGLSHRYPNGFAALEGVDLTVREGEFL
                     AVLGQNGSGKTTLVKHFNGLLKPTEGTVKVEGEETAEQGMLRLGQRVGYVFQNPDNQI
                     FSETVFDEVS"
BASE COUNT         1235 a         2038 c         2033 g         1272 t
ORIGIN      
        1 cgcggcacgc tcggcggtag acttcaggga cgatgcgaag cgccgcgacg acgtgcgtgc
       61 tacgaccggc ttcgtcaacg tcagcccagc catcgtaacg gcgttcaacg ggtggtgcga
      121 gatgtccctc gaccagcgcg cactcgaccc gtccgtgctc gcgagcatgc tcgacgccgc
      181 ccaggaggcg accgcccgtg tcgctacaga agaagacgtc tccgtcgagt gggagaagtt
      241 atggcgcata gagcccatcc ccttcgacga cgagttgatc tcactcgccg aagaggccgt
      301 aaacgagatc gcaggacaat ctcaccggct gccgagcgga cccctgcacg acgccgcaga
      361 gatggcgcgc ctcatgccga cggttatgct tttcgtcaag agcctgcggg gactcagcca
      421 caccaaggac gaggacacgc cggaagaaga tctggagctc tccgtccagg cgctccaccg
      481 gctcgcggtc aagacgatga actgggcagg aaaataggtt tcaggctaca ggcatcaggc
      541 gtcaggaaaa cctccccctg aaacctgatg cctgagcgac cgaagggagc gacattggaa
      601 gagttcaagg atctggcggt aaacaacgag gcggagttgc agcggatcaa atccggagcc
      661 atggcgtctt ccctgtacaa cgaggacctc gcgccgaccg gccccgagca gcggacgtgg
      721 acgacgtaca acatcgctgc gctctggatc gggatggcca tagtcattac gacctatacg
      781 ctggcttcgg gcctcatggc cgctgggatg aactggtggc aggcgctgct taccatctct
      841 cttggcaacg tactcgtcct gatcccgatg ctcctcaacg cccatgcggg gaccaagtac
      901 ggcgtccctt ttcctgtctt cgtccgtgct tcgttcgggg tgaggggggc caacttcgcg
      961 gcgatggcaa gggcgcttgt ggcctgcggc tggttcggga tacagacctg gatcgggggt
     1021 ctcgccctcg acgcgctcat gaaggccggc tggggcggat gggaaagcgt ccctggacat
     1081 tcgttcataa ccttcctcgt cttctgggcg atacagctcg tgatcatcct tcgcggcatc
     1141 gagggggtca agatcttcga gtccctctcc gccccgctgc ttctcggcgg cagccttgtg
     1201 cttctgatct gggggttcag cgcgggcggt ggcatcgcca acgtgttcga gacatcggcc
     1261 aagctccagc agggcgacgc gccgttctgg gctctgttct ggccttctct ggcggcgaac
     1321 gtggggtatt ggatcacgct ctccctcaat atccccgact ttacccgcta cgcgaagacc
     1381 cagcgctcgc aggtcctcgg acaggctctc gggctaccgc tcaccatgac ggccttttcc
     1441 ttcatcggga tagcggttac ggcggcgacc atcgtcgtgt acggcgaggc gatatgggac
     1501 ccggttgtgc tcgtcacgaa gctagctgag aacgcgccgt ggctcctcat cctggctatg
     1561 atcatcatcg cgatcgccca gatctcgact aacatggcgg cgaacgtggt ttcgccctcg
     1621 ttcgacttct cgaacctggc cccgaagtac atctcgttca ggaccggtgg catcattaca
     1681 gccctgatcg gcatcatctc gttcccgtgg gtgctcttca ataacgtcgg cgcctatatt
     1741 ttcacctggc tggtcggcta cggcagcctg ctcggggcga tcggggctgt catgatcgcg
     1801 gactactgga tcgtgcgccg tcgccagctc gacctggccg aactctacaa gctggacggc
     1861 cgctactcgt actcgaacgg ctggaactgg cgcgcgatag ccgccgtgtt tatcggggtg
     1921 atccccgtcc tccccggctt cttcaaggcg gcaacgactc ccggcttcgc aggcgtgttc
     1981 caggacccga cgtttatcga gagcctgtac aactacggcc tgttctttac ctttggcgtc
     2041 gccggcctcg cgtacctcgc gctatccatg atcggtggcc gcgccgccga accggccagc
     2101 gagccggaag cgacgtaagg tatcgccgat gccggacgca tccactttcg ccctcttcgt
     2161 cgccgcagcg ctggtgatgc tgatcgtgcc cgggccttcc gttctctaca tcgtcgcgcg
     2221 tagcgtggag ggtgggagga ccgcgggctt cgtctccgtg ctcggcgtac agacgggcgc
     2281 cctggttcac atcgccttcg ccacgctcgg gctctctgcg atcctcgctt cctcagccgt
     2341 cgccttctcg gtcgtgaagt ggctcggcgc ggcttaccta atctggctcg gcttgaggcg
     2401 gatcttcggc agtgacgagg aggagggcga cgtagccgtc gagccggagc gtctctcgcg
     2461 cgtgttctcg cagggtgtga tcgtcaacac cctcaacccg aagacggcgc tgttcttcct
     2521 cgcttttctg ccgcagttcg tggacccgtc gcgcggcgcg gcatgggcgc agatcctgct
     2581 cctcggcgcc acgttcgtaa tactcgctct gtgctcggac ggcctctacg cccttctctc
     2641 aggtacggcc ggcggctggc tcaggcgcaa gatggagggc gcggacttca ggcgaggaca
     2701 gcggtacgtc tccggcggtg tcctggtagc actgggtgcc gtagccacag ccgcagggaa
     2761 agattaggga ggaaggtatg gacttcggcg tcacgcttca gaccaaccca ccggcgagcc
     2821 gggtcatcga actgacaagg aaggccgagg acctcggctt cgggtacgca tggaccttcg
     2881 actcgcacat actgtggcag gagccctacg tcatacactc gcagatgctc tctgcgacgg
     2941 agaacatagt ggtcggcacc ttcgtcacca acccggtcag cagggaccca tcggtaacgg
     3001 cctcgacctt cgccaccctc aacggcatgt tcggcaaccg caccatctgc ggtataggcc
     3061 gcggcgactc cgtggtgcgc gtccttggcc gcaagcctac gacgctgaag cagctcgaag
     3121 aggcgatgca cgtcataaaa gagctggccg agggaaggga ggtcgactac ggcgacgtca
     3181 aagtacgcat cccgtgggtg gaggacggaa agctcgacgt gtggatggcc ggctacggcc
     3241 cgaaggcttt gagtctcatc gggcgaaagg ccgacggctt catcctgcag ctggctgacc
     3301 ccgtgatcct ggagtggacc atgcagcacg tccacgacgc ggcgaaagaa gcaggccgcg
     3361 acccgaagag cgtgaaaatc tgcgtcgcag ccccggccta cgtcggagac gaccttgccc
     3421 accagcgcga ccagtgccgc tggttcgggg gcatggtcgg caaccacgtc gcggatctcg
     3481 tctcccgcta cggcgaggac ggcgatgtcc cgcacgctct gaccgactac ataaaagccc
     3541 gcgaaagcta cgactacagc caccacggcc aggcgggcaa cccctccacc gacttcgtcc
     3601 ccgacgacat cgtcgaccgc ttctgcgtcc tcggcaccgt cgatgaccac gtaaagaagc
     3661 tgaacgagct aaaagctctc ggcgccgacc agttcaacgt ctacctcatg cacgacgcga
     3721 tggaggagac gctctacgct tatggagaag agatcatacc gacgttaggg gcgaagtcct
     3781 tcaggtgaag ggaagctgtc cgaggctgcc atacggacta aactcggaga ctgatcacct
     3841 catgttcgtt gagcaacctc aagaattcgc tgaagtcggg tggaagcggg atcgtagcca
     3901 tagagcatct gcctggtctc ctcgacggcc tgaagtctct catgcggact cttgccgcgc
     3961 caatacctct tctcgtcgtc ttcctgatcg cccaaaggaa cgacagaaaa agcctcacgg
     4021 tccaccttgc gttccatgca agcagtctat agcgcaacca cactcaacac aaccgacaac
     4081 gccagcacgc atccagtagg atcatttcga catctacctc atgcacgacg cgatggagga
     4141 gacgctctac gcttacgtag aggagatcat gccgacgcag ggtgcgatgt ctgcggtgtg
     4201 atctaccgga gcggcaggta gcggacctgt ccctcccact cgccttcgag gctgcactcg
     4261 gcgatcaaca ggatctcctc gatggctaat ccggctggca tgccgcggcc cacttcgaag
     4321 acccctggca tcggtttgcc ctcccgcact ctctcgtacg cgtagcgggt catggtcgct
     4381 acgtcctgag tcagtagtac ccggccttct tgcgctgccc attccagcac ggccggatct
     4441 tccgctcccg acagacccgc atcctggacg cgaataatgt ctacttcggg gttgcggcgt
     4501 agaacgcctc gcactatatc gtagttgagg ttctcatcgg cagccagccg gatcaacggc
     4561 tctattttgc gcggcgcgcc agcaggcgag cacgcactcc cgccggatcg aacctaccct
     4621 cgtttgccag acgtgcttcg gcggcaaact gctggcgtag ccgaagatat tcatccacct
     4681 ccagctgatg acgtaggtag taaccgatca cggcgtagac gtcggcaaga cggagagaag
     4741 ggtactgctg cgcgagttcc tccgccgtcg caccctcctg gaacgcagcg accacagtat
     4801 ctagcgtaac gcgtgtaccc gtgacacgca cgacaccgtt acggtcggtg gtcagaggga
     4861 ttggttcggg cgcaactgct aacgacatga tcaggaatta tagcctgatg tggcaagtgg
     4921 tcctagcctg ccggttttgc ccgaaagtat ttactgcagt ggtcgactac aactcggctc
     4981 gatgtgttcc aagacgatct gctgcaggcg ctccgtcgtc gagggcgaaa agtactgttt
     5041 gcctgtctct tcgtctactc gcgccgggac gttttcgacg atgtagacct tgccatccaa
     5101 ctccagggcg tatgtgaccc gacgttctac gagtctctct tcttgtctca ggctcatttc
     5161 gatccccttt ccctgaagtc gatccaccgt gcttaatccg gcccatagaa gtttacgatc
     5221 ttgaccagcg ccttcggaga tggctgcatc gtctcccgta ggttacatcg ttttctgcct
     5281 cgaagatccc tatgtggcgc ctaaatctgg tagcgggtag cggagaggac cacttataga
     5341 atattctttg tagatgggga ggtacatagg atgacggaac ccgcaattag cgtccagggt
     5401 cttggttacc ggtacagggg gcagcagaag cctgcgctgc aggggattga cctggaggtg
     5461 gccgaggggg agttcgttgt ggtgatgggt ccttcggggg cggggaagag tacgctttgc
     5521 gtctctttga acggtttgat cccccacttc caccgcggcc gcatggaggg ggaggtaaag
     5581 gttcgcggaa gatctacccg cgatggcaag gtcggggagt tcgcgaagga ggtcgggttg
     5641 gtcttccagg acttcgaggc gcagctgttc tcgacgaacg tagccctcga agtcgccttc
     5701 gggccggaga acttcgccgt ggagcacgac gagatgctca ggcgcgtgaa ggacgtgctc
     5761 gggcaggtgc gcctcgaggg gttcgagggc aggcagcctg cgacgctctc gggcgggcag
     5821 aagcagaggc tcgccatagc atcggtgctc gcgatagagc cgcggatact ttgcctcgac
     5881 gaacctacta ccgacctcga tcctgcgggc aagctgggtg tcttcgagat cgccgaagag
     5941 ctcaaggacc gcgacgacgt caccctgatc gtggtcgagc acgagaccga agagacgctc
     6001 gacgcggaca ggatcattgt cctgcgcgac ggtgaaataa tcgccgaccg ccctgcccgc
     6061 gaggtcctca gggacgtcga gctcctggag gagtccggtg tcatgccgct ccaggtggcc
     6121 cgcttcttcc acgagatggg cctgtggcag gcgcagctac ccctgacgcc ggaggagggc
     6181 tcccaggagt tccgcaggcg cggttggagg gtaaacccgg accaccacag ggcactggta
     6241 gccgaggacg ggcagagggc cgagggatac ggggaaccca tcatcgagat cgacggcctc
     6301 tcgcatcgtt acccgaacgg tttcgcggcg ctcgaaggtg ttgacctcac cgtgcgcgag
     6361 ggcgagttcc tggctgttct ggggcagaat ggctcgggga agaccacgct tgtcaagcac
     6421 ttcaacgggc tgctcaagcc cacggagggc acggtaaaag tcgagggcga ggagaccgcg
     6481 gagcagggga tgctcaggct cgggcagcgc gtcggctacg tcttccagaa ccctgacaat
     6541 cagatcttct ccgagacagt cttcgacgaa gtctcttt
//
LOCUS       DATAIR010000754         7769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_166647, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000754 DATAIR010000000
VERSION     DATAIR010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166647"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..440
                     /locus_tag="VHM69_17935"
     CDS             60..440
                     /locus_tag="VHM69_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2742326.1"
                     /translation="MQKELVKPELLSGIEVGRESEEMCLLARLFNGFANSTRLSILLL
                     LVQRGEMKVGELVDELGAPQPRVSDHLRCLAWCGYVQVRREGRNAYYSVADERVMQML
                     RLGEELLQDNLEHVGSCADIEKDC"
     gene            complement(558..1739)
                     /locus_tag="VHM69_17940"
     CDS             complement(558..1739)
                     /locus_tag="VHM69_17940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742327.1"
                     /translation="MGKMIRIAGMGLSGLMVNRGRSALSMLGIVIGVFAVILLTSLGN
                     GVQEAITGKLTGVGSNLLTISPGNAEAQGRGPFAAASASTLTRKDVEKVENLPTVAAA
                     SASVPTAALAGTQTIPLTGVDPSYERIRSVDLASGRFVEHSDEVVLETPVARDLLKSG
                     PKEAVGRTIEIQGDSYEVVGVLEVTKPAFGPPVPETSYVITDDALALSGARNVGQIVA
                     EARSAGSVDRAAKDIGAALKSAHGGTEDFNVLTQKDLLSVFTLITDLLTVLLGGIASI
                     SLLVGGIGIMNIMLVSVAERTREIGIRKAIGATDTDVLFQFLFEALLLTLIGGLAGIG
                     LGIGVSALLPEINPIIPTSVTGTSIVLAFGVSALVGLVFGVLPAYRSARLQPVEALRR
                     E"
     gene            complement(1739..2428)
                     /locus_tag="VHM69_17945"
     CDS             complement(1739..2428)
                     /locus_tag="VHM69_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742328.1"
                     /translation="MLVIAARDLTKVYGRGENKVEALAGMSMNVEPGEWIAIMGPSGS
                     GKSTLMNLIGLLDRPTSGAYTLDGRDVSRLKGGELARARRDLIGFVFQSYNLLSRQSA
                     LANVELPMIYAGIGRKERRERALGVLERVGLAGRVHHRPTELSGGQNQRVAIARALVN
                     NPALLLADEPTGNLDTASGAEILDLFAELNASGVTVVVVTHDAEVAARAERMVELRDG
                     RIVADERSEVA"
     gene            complement(2431..2646)
                     /locus_tag="VHM69_17950"
     CDS             complement(2431..2646)
                     /locus_tag="VHM69_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742329.1"
                     /translation="MCAIFGIIFLGGITALVMWTVFRGFPGPGRRPPGFSMAGRGDAA
                     EEILHQRFARGEIGAEELQRALRVLRS"
     gene            complement(2694..3068)
                     /locus_tag="VHM69_17955"
     CDS             complement(2694..3068)
                     /locus_tag="VHM69_17955"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2742330.1"
                     /translation="MHEAMNILTEAWSLASLIYLEGPPWDHRGWDGPGSGGSWWPWIL
                     FPILFWGGILSFIAWIVTRLYPRSRDRSESPRDSAEEILRERFARGEITADEYERSLD
                     ILRGGTARRDYGEDQQPGERES"
     gene            complement(3121..3420)
                     /locus_tag="VHM69_17960"
     CDS             complement(3121..3420)
                     /locus_tag="VHM69_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2742331.1"
                     /translation="MDGPDLFWGPWIIWPVLFWITVIWLMKLGVSRRTHFRHRSGDTA
                     EEILRERFARGEITAEEYERSIGALRGRFPDKDYEDYVREAEEELRSEREDSPRQ"
     gene            complement(3513..4598)
                     /locus_tag="VHM69_17965"
     CDS             complement(3513..4598)
                     /locus_tag="VHM69_17965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2742332.1"
                     /translation="MKRLWRPPGGLGAKLFVSHFLVALVGALTMFTVILVVAPLVFGN
                     LSGGEAVSPVRAFGQALLYSLLVAVLAATAVSSIASLLVSRRVVDPMRDVLSAARRIA
                     SGRYGERVPVRRLGELSEGFNAMARALDEAERRRADVISDLSHEMRTPLSTLRGYMEG
                     LAEGVIEPSDEVWDILNTETERMERLVDDLRQLSQAEAGRLPLDIVPVSPAEPVRRVT
                     ELMMPLFEEKRVRLESTVPGGTPPALMDADRVVQVLTNLLSNALRHTPPGGLVLLEVG
                     DGDGEVTFRVTDTGEGISSEHLPHVFERFYRADKSRSREGGGSGVGLAISRALVEAMD
                     GRMWVVSPGAGKGATFSFTLPINRAES"
     gene            complement(4595..5287)
                     /locus_tag="VHM69_17970"
     CDS             complement(4595..5287)
                     /locus_tag="VHM69_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742333.1"
                     /translation="MTIRALIVEDEKYLVDLLRKYLEREGFEVDEALDGPAALEVARR
                     SEPDVVVLDWMLPGLDGMGVLRELRRFSDAYVIMLTAREGEVDRIVGLSTGADDYLVK
                     PFSPGELVARIRAMLRRPRGGSTTDPEDPAMTFGDLSIDPARREVRLGRDDLQLTAME
                     FDLLATLASRPGLVFSRRQLLARVWGDDYFGSDHVVDVHVANLRKKIEDDPSNPRYVQ
                     TIRGVGYRFSRP"
     gene            complement(5333..5701)
                     /locus_tag="VHM69_17975"
     CDS             complement(5333..5701)
                     /locus_tag="VHM69_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742334.1"
                     /translation="MAAQHGYRFPEDLARHASKERFLPDELRGACRDAGEGLGREDAR
                     EARFRSAAEMVEMWRGLDLPASFAPYALRDARLGYIGGYAQILVSGELPEVRTARAAE
                     ARWGPRWRERLEAARRRAGH"
     gene            complement(5740..6810)
                     /locus_tag="VHM69_17980"
     CDS             complement(5740..6810)
                     /locus_tag="VHM69_17980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2742335.1"
                     /translation="MRWTHVLDEYFRRRAARDQFSGAVLITQGTSRLYARAHGYASRA
                     WRVPNTLDTRFDTASVTKLFTSIATLQLIDRGLLAFDTPVVERLGLEGTTLSKEVNVL
                     HLLTHTSGIGDDADEEAGERYEDLWKTKSNYSVVETRDFLPQFVNKPANFPPGQGCRY
                     CNCGYVLLGLLIETISGMTYRDYIRAHVFAEAEMTTSDFFRTDRVNHNVAEGCDPIRD
                     ATGAIVDWKKNIYSFPPIGSPDSGAHVTVVDLDRFLRAVRAGDLLSPELTGAFFTPQV
                     VHRTWDTWTEMYGYGLWFYVDQASKVVCFEKEGVNVGVSGLIRYFPDQDIAVTLLSNM
                     EDGAWDPVWKIHEMVVDGQMTR"
     gene            7330..>7769
                     /locus_tag="VHM69_17985"
     CDS             7330..>7769
                     /locus_tag="VHM69_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742336.1"
                     /translation="MGKASRIFKSERRLGLYLLAALCVALGAIFVGLSVLETQSEPVK
                     SAATPEVASSPETLSNDARVESQARIEGQAARRAAEEKAARAEAAQQEAAREQAAAAQ
                     AAAPERPAPERPAPERAVAEPEPAAHVAPADTTMSLSVPALGISG"
BASE COUNT         1443 a         2481 c         2391 g         1454 t
ORIGIN      
        1 agggtacaca ctccggaacg ggcggtgtat agtaagaatg gacgtggaag gagcgccttg
       61 tgcaaaagga attggtcaag ccggaactct tatccggcat cgaggtaggt cgcgagagcg
      121 aagagatgtg tcttctcgcc aggttattca acggtttcgc gaactcgacc aggctctcga
      181 tcctgctgct tctcgtgcag cggggcgaga tgaaggtcgg ggagctggtt gatgagctcg
      241 gggcgccgca gcccagggtc tcggatcacc tgcgctgcct ggcatggtgc ggttacgtgc
      301 aggtgcggcg cgagggcagg aacgcctatt actcagtggc ggacgaacgg gtcatgcaga
      361 tgctcaggct cggcgaggaa ctactacagg acaacctgga gcacgtaggg tcctgcgccg
      421 atatagagaa ggactgctaa gaacacaacc cggagtccaa gcactgtttt cgccagggtg
      481 cctgggacgc gtctgcacag accctgcggg tgtcccctcg gggacacccg caggattggc
      541 tgaggcggta aacccgttta ctctctcctc agggcttcga cgggctgcaa ccttgccgag
      601 cggtaggctg gcagaactcc gaagacgagc ccgaccaggg cagacacgcc gaaggccagt
      661 acgatcgacg tacccgtcac cgaggtgggt atgatcgggt tgatctcagg cagcagggcc
      721 gagaccccga taccgaggcc gatgccagcg agacccccga tcaaggtcag tagcaatgcc
      781 tcgaagagaa actggaaaag cacgtcggtg tccgtcgcgc cgatggcctt gcggatgccg
      841 atttccctgg ttcgctccgc gacggagacg agcatgatgt tcataatccc gattccgcca
      901 accagcaagg atatgctcgc gatgccaccc agtaggacgg tgaggaggtc cgtgatcagg
      961 gtgaagacag acagcagatc cttctgggtc aacacgttga agtcctccgt gccgccgtgg
     1021 gcactcttta gcgcggcccc gatatccttc gccgcgcggt ccacggagcc ggcgctcctg
     1081 gcttcggcga caatctggcc cacgttcctg gctcccgaga gcgcgagggc gtcgtccgtg
     1141 atcacatacg aagtctcggg taccggcggt ccgaatgccg gcttcgttac ctctagtaca
     1201 ccgacgacct cgtagctgtc gccctgtatc tcgatcgtcc tgccgacggc ttctttcgga
     1261 ccggatttca ggagatccct cgcgacgggc gtctccagga cgacctcatc agagtgctcc
     1321 acgaagcgcc cggaggcaag gtccacggag cgtatccttt cgtaggaggg gtcaacaccg
     1381 gtaagcggga tcgtctgcgt gcccgccaga gccgcggtcg ggacacttgc cgaggccgcg
     1441 gcgactgtgg gcaggttttc gaccttctct acgtccttcc gggtgagtgt actcgcggag
     1501 gcggcggcga acgggccgcg gccctgcgcc tcggcgtttc ccgggctgat cgtgagtagg
     1561 ttcgaaccaa cgccggtaag cttgccggtg atcgcctcct gcaccccgtt gccgagagac
     1621 gtgagcaaga tgacggcgaa gaccccgatg acgatcccga gcatgctcaa ggcgctgcgt
     1681 ccccggttga ccatgagccc cgagaggccc atcccggcga tcctgatcat ctttcccatc
     1741 acgccacctc cgatctctcg tcggcgacga ttcgcccgtc gcgaagctcc accattctct
     1801 ccgcccgcgc ggcgacctcc gcgtcgtgcg tgacgaccac gaccgtgaca cctgaggcat
     1861 tcaattccgc gaacaggtcg aggatctcgg ctccggacgc agtatccagg ttgcctgtcg
     1921 gttcgtcggc cagcagcagg gcggggttat tgaccagtgc gcgggctatg gcaacgcgtt
     1981 ggttctgccc gcctgatagc tcggtcgggc gatggtgcac ccgtccggca agcccgaccc
     2041 tctccaggac accgagggcc cgctctctgc gctctttcct gccgattccc gcgtagatca
     2101 tcggaagctc cacgttcgcg agcgcgctct gccgggagag aaggttgtag ctctggaaga
     2161 cgaacccgat gagatccctg cgggcgcgtg ccaactcacc acccttgagg cgggagacgt
     2221 cgcgcccgtc gagggtgtag gcgccggacg tcggacggtc cagcagcccg atgaggttca
     2281 tcagggtgct cttgccgctg cccgacggcc ccattatggc gatccattcg ccgggctcga
     2341 cgttcatgct catcccggcg agcgcctcca ccttgttctc tcccctaccg tagaccttcg
     2401 taaggtctct ggctgctatt accagcattc ctaactcctc aacactctca gggcccgctg
     2461 cagttcctcg gcgcctatct cgccgcgggc gaacctctgg tgcaggatct cctccgcagc
     2521 atctccccgg cccgccatcg aaaagccggg cggccgtcgt ccgggtcccg ggaagccgcg
     2581 gaacacagtc cacatcacga gggccgtgat gcctcccagg aagatgattc cgaatatcgc
     2641 gcacataatt cctcaccctc tcttcgtaag cgccgcttat ggaagcggtt ctctcagctt
     2701 tctcgttccc cgggctgttg gtcttcgcca tagtctctcc gggctgtccc tccgcggagg
     2761 atatccaggg atctctcgta ttcatcagcg gtgatctcac cccgcgcgaa acgctccctc
     2821 aggatctcct cggcggaatc tctaggcgac tcgctgcggt cacggctcct ggggtagagg
     2881 cgggtaacga tccaggctat gaaggagagg atgccgcccc agaaaaggat ggggaagagt
     2941 atccacggcc accacgagcc gccagagcca ggtccgtccc aaccgcggtg gtcccacggc
     3001 gggccctcca gatatatgag ggaagcgagg ctccacgctt ccgtaaggat gttcatcgct
     3061 tcgtgcatct cggtctaccg tttctatgtt cttcgtgatc gaacggggct ttcgtagcca
     3121 tcattgtcgg ggactatctt cccgctcgga ccgtaactcc tcttcggctt ctctcacgta
     3181 gtcttcgtaa tctttatccg ggaacctccc acgcagggct ccgatggacc tctcgtactc
     3241 ctcggcggtg atctcacccc gcgcaaagcg ctccctgaga atctcctccg ccgtgtcccc
     3301 actcctgtgc cggaagtgcg tcctgcgtga aacacccagc ttcatgagcc agattaccgt
     3361 tatccagaac agaacaggcc agattatcca cggaccccag aacagatccg gtccatccat
     3421 cgcgatcgtc tcctttcctg gctgaacatc gttcgtggtc ccaggatagg cgcgggatct
     3481 caagcccgga tctgcctgag atcaagattg gatcaagatt ccgcccggtt gatgggcagg
     3541 gtgaaggaga aggtcgcgcc tttgcctgct ccgggactca cgacccacat cctcccgtcc
     3601 atcgcctcca ccagcgccct ggatatggcg agacccactc cagagccgcc gccttctctg
     3661 gaacgggact tgtccgccct gtagaaccgc tcgaagacgt ggggcagatg ttcggaggaa
     3721 atgccttcac ctgtatccgt caccctgaag gtgacctcgc cgtcaccgtc tccaacttct
     3781 agtaagacca gtcctccggg aggagtatgg cggagggcat tggagaggag attggtcagt
     3841 acctgtacga cacggtcggc gtccatcagc gcaggtggtg tccctccggg gaccgtgctc
     3901 tccagccgga cacgcttctc ttcgaagagc ggcatcatga gctccgtgac acgacggacg
     3961 ggctcagcgg gtgaaacggg aacgatatcg agggggagcc ggccggcctc ggcttgagag
     4021 agttgcctca ggtcgtccac cagccgctcc atccgctcgg tctcggtatt caggatgtcc
     4081 cacacctcat cagatggctc gataacccct tcggcgagcc cctccatgta gccccgtagc
     4141 gtggagagcg gggtacgcat ctcgtgggag aggtcggaga tcacatccgc cctcctcctc
     4201 tcggcctcgt ccagggcccg cgccatggcg ttgaagccct ccgaaagctc tccgagcctc
     4261 cgcacgggca cgcgctcgcc gtagcgcccg gaggcaatgc ggcgagccgc cgacaacaca
     4321 tctcgcattg ggtcaacgac gcgtctcgaa acgagcaggc tcgcgatgga ggatacggcc
     4381 gtcgccgcga gcaccgccac gagcagggag tacagtagcg cctgcccgaa agcccgcacc
     4441 ggcgagacgg cctccccgcc ggagaggttc ccgaagacca aaggcgccac gaccagtatg
     4501 accgtaaaca tcgtgagcgc ccccaccagg gcgaccagga agtgggagac gaagagcttg
     4561 gcgccgaggc cgccaggcgg acgccagaga cgtttcatgg ccggctgaac ctgtacccga
     4621 cccctcggat agtctggaca tagcgagggt tcgaggggtc gtcctcgatc tttttccgta
     4681 ggtttgcgac gtgtacgtcg accacgtggt cgctgccgaa gtaatcgtct ccccataccc
     4741 gcgccagcaa ctgcctccgg ctgaagacga gtcccggccg cgaagccagg gttgcgagca
     4801 ggtcgaactc catagccgtg agttgcaagt cgtctcgccc gagacgcacc tctctacggg
     4861 ccgggtctat actcaggtcg ccgaatgtca tggctggatc ttcggggtcc gttgttgagc
     4921 cgccgcgcgg ccttcggagc atggccctga tccgggcgac cagctctcct ggtgagaagg
     4981 gctttacgag gtagtcgtcc gcgcccgtgg agaggcccac gatcctatcc acctccccct
     5041 cacgcgctgt gagcatgatg acgtaggcgt cggagaagcg tctcaactcc cgcaagaccc
     5101 ccatgccgtc gagccccggc agcatccagt ccagaaccac aacgtcgggc tcggagcgcc
     5161 gggcgacctc gagtgctgcg gggccgtcga gggcttcgtc gacctcgaag ccttcccgct
     5221 ccaggtactt ccggagcaag tccaccaggt acttctcgtc ctctactatt agagcgcgaa
     5281 ttgtcatgga gctgttgata gtctaaccgt tccacacagg gacgacggat ggtcagtggc
     5341 cggctctccg gcgggcggct tcgagtctct cccgccaccg cggcccccat cgcgcttctg
     5401 ccgcgcgggc ggttcgcact tcgggaagct ccccggagac gaggatctgt gcgtagccgc
     5461 ctatgtagcc gagacgggcg tcgcgcaggg cgtacggagc gaaggaagcc ggcaggtcga
     5521 gcccgcgcca catctcgacc atctccgccg ccgaacggaa gcgggcctcc cgcgcgtcct
     5581 cccgtccgag tccctccccc gcatcccggc aagcaccgcg cagctcatcg gggagaaacc
     5641 gctctttcga agcgtggcgc gccaggtctt ctgggaaccg gtatccgtgt tgtgcggcca
     5701 accgttctga ctcactttcg aacttcggta cgaggctatt taccgggtca tctggccgtc
     5761 gaccaccatc tcatgaattt tccagacggg atcccacgcg ccatcttcca tatttgatag
     5821 gagtgtgacg gcaatatcct gatctggaaa atagcggatc aggccactga cgccaacgtt
     5881 caccccctct ttttcaaagc agacgacctt gctcgcctga tccacgtaaa accataaccc
     5941 gtacccatac atctccgtcc aggtatccca cgttcgatgc acgacttgag gtgtgaagaa
     6001 tgcgccggtc agttcgggcg aaaggagatc gcccgccctc accgcccgca agaatcggtc
     6061 caggtcgacg accgtgacat gggcgccact atccggcgac ccgatcggtg ggaaggagta
     6121 gatgttcttt ttccagtcca cgatggcccc ggttgcgtcg cgaatcgggt cgcatccttc
     6181 ggcgacgttg tggttgacgc ggtccgtccg gaagaaatcg gaggtggtca tctccgcttc
     6241 ggcgaatacg tgggcgcgga tgtaatcgcg gtacgtcatc ccgctgatgg tttcgatcag
     6301 gaggccaagg aggacgtagc cacagttgca atagcggcac ccttgtcctg gggggaagtt
     6361 ggccggcttg tttacgaatt ggggcaggaa gtcgcgggtt tcgacgacgg agtagttgga
     6421 cttcgtcttc cacaggtctt cgtaccgttc gcccgcctct tcgtcggcgt cgtcgcctat
     6481 gcccgaggtg tgggtcagca agtgaaggac gttaacttcc ttcgagagcg tcgtgccttc
     6541 caggcccagg cgctccacca cgggagtgtc gaaggccagc agccctctgt cgatcagctg
     6601 taacgttgcg attgaggtaa agagtttggt aaccgaggcc gtatcgaacc gcgtatcgag
     6661 ggtgtttggc acgcgccatg cgcggctggc gtaaccgtgg gcgcgggcgt acaaccggga
     6721 tgtgccttgc gtgatgagca ccgcgccgga gaactgatcc cgggcggctc ggcgccgaaa
     6781 atactcgtcc agtacgtggg tccaacgcat gacgtccttc ccaagcgctc catcgtgccc
     6841 cggtaacctt cgacatcgcc gtgtaggtga gccggagagc cgcagtgccc cttcaacgca
     6901 acatggtcgc accttcggac gatcgtagca aagtgccaat gaaggatcaa tcaggggcct
     6961 tgcgacggac cagagtggca tgttatcccg tggtcggaag atgatccagc acggggaact
     7021 cacggacgag gcgttggggc gcggtagggg ccgcttctag agcggtgtgg acggatctat
     7081 ccgtccacac atagcgccca ggctaccagc tctgtgtcgc gtctctgcga gtcgccctac
     7141 gagctctcac accaacctcc accgccgccc ggatcgcgaa gaacccaggc tccagccgat
     7201 gttacgaaaa cgttacaaaa aagttgatat ttatggttgt gctcttagat acggtagttt
     7261 aatgtggtga tgcttccggc agggaaccag cactaacaaa acattggcag tggggaggtt
     7321 aggcgaggga tgggcaaagc atcgaggatc ttcaagagcg agcgtcggtt gggcctgtat
     7381 ctgctggcgg cgctctgtgt agcgttaggc gccatcttcg tcgggttgag cgtacttgag
     7441 acgcagagtg agccggtaaa gagtgcggcc acccctgagg tagcctcctc gcccgagact
     7501 ttaagcaatg acgccagggt cgagtcccag gcgcggatag aagggcaggc ggccaggcgg
     7561 gctgccgagg aaaaggcggc cagggcagag gccgcacagc aggaggccgc acgggagcaa
     7621 gccgcggcgg cgcaggccgc cgcgcctgag cgtcccgcac ctgagcgacc cgcgccggag
     7681 cgcgccgtcg cagagcccga gcctgcggcc cacgtagccc ctgctgacac caccatgtca
     7741 ttgagtgtgc ctgcgttggg catctccgg
//
LOCUS       DATAIR010000755         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_221158, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000755 DATAIR010000000
VERSION     DATAIR010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221158"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VHM69_17990"
     CDS             complement(<1..637)
                     /locus_tag="VHM69_17990"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742337.1"
                     /translation="MRLDRPDSYWKKDVALFLSRRFALLIKMLYPVAIIVPVAASGMP
                     AKYAAAVIGIVAIMAGTFGAGESLTIDLNDGILVRVALTPLSPYRIVFEILAVNAVLD
                     FVQLLPALLIVYLLHPVSIVWMLAASLAVFATLVTANCIGVFIANFTSSPADVLLYAT
                     AILFPLIYLSGFFRNQHPTGILHTLRGFVPFAYTDDAMKTVFGLTTGTTPLE"
     gene            complement(627..1637)
                     /locus_tag="VHM69_17995"
     CDS             complement(627..1637)
                     /locus_tag="VHM69_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742338.1"
                     /translation="MRITTGPRAGRYNSHTMRSGAQNPQPALEAKNMSRLYRSSGRGV
                     SGVCISVAPGEVFGLMGPNGSGKSTLLRILSTAIAPDSGAFSVGGADGLKEKRTVRAR
                     MGIMVDRPTHYGDLTGRANAYFFARAYGMDEAKAERVLTELFDYFDLAEYADDKVKTY
                     SYGMGKKLALVEALAHGPEVLLLDEPSLGLDYTSQLAFQRRIRDLASAGVAVVLASNQ
                     VDEVESLCDRAAFLHRGKLVAEGTPEELISTVEGVRRIVATLRNPVEYGRVAEVGGVQ
                     RIVPEGRDLIFHCDERPGMVADVVAGIVNAGGDIVNLSVQRPNLEDVFVKLTGEALRD
                     EA"
     gene            complement(1720..2518)
                     /locus_tag="VHM69_18000"
                     /pseudo
     CDS             complement(1720..2518)
                     /locus_tag="VHM69_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2731..3012
                     /locus_tag="VHM69_18005"
     CDS             2731..3012
                     /locus_tag="VHM69_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742339.1"
                     /translation="MGCRARPSRALHALVLKHPGALRRMFWPPGEISLSEAYIHDDFD
                     IEGEIEAVFPWPTTSSPGGAASPGAFARPDGFSPCPRRAYRAQGGALAG"
     gene            3002..3898
                     /locus_tag="VHM69_18010"
     CDS             3002..3898
                     /locus_tag="VHM69_18010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX2742340.1"
                     /translation="MLGRRHSKARDRRAVTYHYDVPGEFYALWLDQRMVYSCAYFATP
                     DEDLDAAQERKLDLICRKLRLRRGEKLLDIGCGWGRLIIHAARRYGVEALGITLSEPQ
                     AELANERIRRAGLEDRCRVEVRDYREIEDWGAYDKLVSVGMFEHVGEARLAVYFSRAY
                     RLLRPGGVFLNHGISRNPGTPERRGPTFISRHVFPDGELVPIGTTLSAAEGRGFEVRD
                     VESPREHYVLTLGHWVRRLEDNAEEARRIVDETTYRVWRLYMSGSAHGFRTGRINVYQ
                     ALLVRPDRGESGLPLTRADWYA"
     gene            4107..4292
                     /locus_tag="VHM69_18015"
     CDS             4107..4292
                     /locus_tag="VHM69_18015"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEX2742341.1"
                     /translation="MKDTHITWDPEADRRIRRSPFLMRRFIRKRIEDRVASRGRTHVT
                     EADVDESASMYRQYRFK"
     gene            complement(4636..>5202)
                     /locus_tag="VHM69_18020"
     CDS             complement(4636..>5202)
                     /locus_tag="VHM69_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2742342.1"
                     /translation="VVVDGEEITAEGVDANRIRRRIGIVYQSFNLFPHMTVLRNVTLA
                     PREAMKMDRAEAERQARALLARFGLLDKQDEYPDRLSGGQQQRVAIVRALAMRPHLML
                     LDEVTSALDPELVAEVLAVIRELAREGMTMLIATHEMGFARDIADRVCFLDAGRILEQ
                     GPPERIFTSPHDPRTHQFLRRIIEAGRL"
BASE COUNT          958 a         1620 c         1638 g          986 t
ORIGIN      
        1 tctcgagcgg cgtcgtgccc gtggtcagtc cgaagaccgt cttcatggcg tcgtccgtgt
       61 aggcgaaggg gacgaagccc cggagcgtgt ggagaatgcc cgtcgggtgt tggttgcgga
      121 agaagcctga gaggtagatc agggggaaca gaatcgccgt cgcgtagagg agcacgtcgg
      181 ccggcgagga ggtgaagttg gcgatgaata ccccgatgca gttcgcggtc acgagcgtcg
      241 cgaagacggc gaggctggcc gcgagcatcc acacgataga gacggggtgc aggaggtaga
      301 cgatcaggag cgcgggcagc agctgtacga aatccaggac ggcgttgacg gcgaggatct
      361 cgaagacgat cctgtacggc gagagcggtg ttagcgcgac gcgcaccagt atgccgtcgt
      421 tcaggtctat ggtcaggctc tcgccggccc cgaacgtccc cgccattatc gcaacgatgc
      481 caatgacggc ggcggcgtac ttcgcaggca tgccgctcgc agccacgggc acgattatcg
      541 ctacggggta gagcatcttt atgagaagcg cgaatcgcct ggagaggaag agcgccacgt
      601 cctttttcca gtaggagtcc ggcctgtcaa gcctcatcgc gcagcgcctc ccctgtcagc
      661 tttacgaaaa cgtcctcgag gttgggcctc tgcaccgaga ggttcacgat gtccccgccg
      721 gcattgacga tgccagccac cacgtcggcg accatgcctg gacgttcatc gcagtggaag
      781 atcaagtccc gcccctcagg cacgatgcgc tggacgcctc cgacctccgc tacccggccg
      841 tactccacgg ggttgcgcag cgtcgcaacg atgcgcctga cgccctcgac ggtggagata
      901 agctcctcgg gcgttccttc ggcgactaac tttcccctgt gcaaaaaggc tgccctgtcg
      961 cagagggact cgacctcgtc gacctggttg ctcgcgagca cgacggccac gccggcgctg
     1021 gcgaggtccc tgatcctgcg ctggaacgcg agctgcgagg tgtagtcgag cccgagcgag
     1081 ggctcgtcca ggagcagcac ctcgggcccg tgcgccagcg cctctacgag cgcgagcttc
     1141 ttgcccatcc cgtacgagta tgtcttcact ttgtcatccg cgtactccgc caggtcgaag
     1201 tagtcgaaga gctcggtgag cacacgttcg gccttggcct cgtccatccc gtaggcgcgg
     1261 gcaaagaagt aggcgtttgc cctgcccgtg aggtcgccgt agtgcgtcgg acggtcgacc
     1321 atgattccca tgcgggcgcg taccgtccgc ttctctttca ggccgtctgc cccgccgacg
     1381 ctgaaggcgc cggagtccgg tgcgatggcg gtagatagga ttctcaaaag ggtgctcttg
     1441 cccgagccgt tcggccccat gaggccgaag acctcgccgg gagcgacgga gatgcacaca
     1501 ccggatacac cgcgtccact gctcctgtac aggcggctca tgttcttcgc ttccagggct
     1561 ggttgtgggt tctgtgcgcc ggacctcatc gtatgagaat tgtagcgtcc tgcccgcggg
     1621 ccggttgtta tgcgcatcac ccgtgctctg atgggttatg gtaacgatgg acgattgata
     1681 gtcgggacgg cgtatgtcta gcgtcactaa ctaactatct caaaaggata gacgatgctg
     1741 acgacggtac cacagagcag gatgcaggcg accttaatca gccccagata gttggcagcc
     1801 ttcttctcgt agcgtaccag gatggagcga cacttcgaca gccagcccag cgtgcgctca
     1861 actacccatc gtcgagccgg ataacgcttg ttcccttcca ggtccttctt ctcttctccg
     1921 atgcgtcgga tatgcgcctg gtagtcgtgg cgctccacca cttcccgagc gggctgttta
     1981 tcgtagccct tatcaagaga cagatgttga ggagcctcct cggtgggttc gggacacgcc
     2041 accacgatgg cctccagcgt ggcttcgagc agcttgaagt cgggtacgtt cgccccggct
     2101 atcaccaccg aaagcggtcc tccatccgcc tcgactagaa gggctcgctt cacccccttc
     2161 ttgcctctat cagtgggatt ggggcctacg aggtcccccc aaaacgagct ttgcccatag
     2221 cgccgtccgc ggcctgccac tcgaagtcca ccccaccgag ttcctcgcag tgctcgatga
     2281 gactctccca gatgaggtcc aacaagccaa gctcgatcca cctctgcaaa gtccgatgta
     2341 ccgaggagtc gtcgggaaac tctcctgtgg gcagtcggtt ccactggacc ccgcttctca
     2401 tcctaaagat gatcgcatcc aaagcggcac gctggtccac gcgagggcgt ccggtgctct
     2461 tgggcggatc atgctctact aggattggtt cgatgatctc ccacaactcg tctggcattc
     2521 gccagatggt gggaagcggt ttcttttggg ttgcgttttc gggcatcgtt gaggcttctt
     2581 tcgataggga tcgcaggcga tgataaccga tcttgagagt tttgagatag ttagtaaggc
     2641 agaggtccat agaagtctct ccttcctcga cagcctgctc ggagagcacc atccgcgcga
     2701 cttcgccgtg cggttgtgga acggtaccgt gtgggctgcc gagcccggcc atcccgcgcg
     2761 cttcacgcgc tcgtactcaa gcaccccggc gcgctgcgca ggatgttctg gccgcccggt
     2821 gagatctccc tctcagaggc atacatccac gacgacttcg acatcgaagg ggagatcgag
     2881 gctgtcttcc cctggccgac gacctcctcg ccaggcggcg cagcctcgcc gggagccttc
     2941 gccaggccgg acgggttctc tccctgcccg cgacgggcgt accgggcgca aggcggcgcg
     3001 cttgctgggt agacgccatt cgaaggcacg tgacaggcga gccgtcacct accactacga
     3061 tgtgcctggc gagttctacg cgctgtggct cgaccaacgg atggtctact catgcgcata
     3121 cttcgccacg cccgacgagg acctcgatgc ggcccaggag cggaagctgg atctcatctg
     3181 ccgcaagctt cggctgcgta ggggcgagaa attgcttgac atcggctgcg gatggggaag
     3241 gctcatcatc cacgccgcgc gccgctacgg ggtggaggct cttggcataa cactcagcga
     3301 accgcaggct gagctggcca acgagcgcat ccgtcgggcc gggctggaag atcgctgccg
     3361 cgttgaggtt cgcgactaca gggagatcga ggattgggga gcctacgaca agctcgtaag
     3421 cgttgggatg ttcgagcacg tgggcgaggc caggctggct gtctacttct cgcgcgcata
     3481 ccgcttgctg cgccccggcg gagtcttcct gaaccacggc atctcccgca atccaggaac
     3541 gccggagcgg cgcgggccta ccttcataag ccgccacgtc ttccctgacg gtgagctcgt
     3601 gcctataggc accactttga gcgccgcgga gggccgcggt ttcgaggtgc gcgacgtgga
     3661 gagcccgcgc gagcactacg ttctcacgct cgggcactgg gtgcgccgcc tggaggacaa
     3721 cgccgaagag gcgcgccgta tcgtcgacga gactacttat cgtgtgtggc ggctgtacat
     3781 gtcaggttct gcccacggct ttcggacggg ccggatcaac gtctaccagg cgcttcttgt
     3841 caggcccgac cgcggcgaga gcgggctacc actgacgagg gcagactggt acgcctgagg
     3901 ggcgggcgtc ctatcggacg gtcctccttg cgcgccccgg aacagcgatg agataagccg
     3961 cttcgccgag gacgaagagc acctgcacca cgaccgcgaa tcaacgctgc tagcctgacg
     4021 cggcagcccc gaccccctcc agcccaacag ccaggtagct gggatagaat gtaagcacga
     4081 ggattcttag agaggggcat gaaacggtga aagatacaca tattacctgg gaccctgagg
     4141 ccgaccgcag gatcaggcgc agccccttcc tgatgcgccg gtttatccgc aagcgtatcg
     4201 aggatagggt tgcctctagg ggacgcaccc acgtcacaga ggcggacgtt gacgagagcg
     4261 ccagcatgta caggcagtac cgctttaaat aggcttccga aagcatcttt cccgaagatc
     4321 tcagcgaagg accagcgtgc tggaaagcgc cagcacgaga aagcattgct tcccaccggg
     4381 gcctgacgcc tgaaacctga aacctttaca aaatatccgg gccgggaagc cgctcttttg
     4441 gcgaacgacc accgacccgg tccgcgaact ttgtagagtc cgcagatggt tgttgactag
     4501 ctagtccact ggaccctttc ctcctagtcg aggtgcaaca accagggtca tgttatcatc
     4561 tctcgcgatt ctcgcgccac cgctttcgga tcatttcgtg cgctcctgcg aacttgccac
     4621 cacatgaagt gggaactaca accgtccggc ctcgatgatg cgccggagga actggtgggt
     4681 ccttgggtca tgcggagatg tgaagatcct ctccggcggc ccctgttcga ggattcggcc
     4741 agcatcgagg aagcacacgc ggtcggcgat gtcgcgggcg aagcccatct cgtgcgtcgc
     4801 gatgagcatg gtcatgcctt cgcgggcgag ctccctgatc actgcgagga cctcggcgac
     4861 aagctcgggg tcgagggcgc tcgtgacctc gtcgaggagc atgaggtgcg ggcgcatcgc
     4921 gagcgccctc acgatggcga cccgctgttg ctggccgccc gagagcctgt cagggtactc
     4981 gtcctgcttg tcgagtagcc cgaaacgagc gaggagcgcg cgggcctgtc tctccgcctc
     5041 ggcccggtcc atcttcatgg cctcgcgcgg ggccagggtg acgttgcgca ggaccgtcat
     5101 gtgggggaag aggttgaagg actggtagac gatgcctatg cggcgcctga tgcggttcgc
     5161 atcgacgcct tcggctgtga tctcctcccc atctaccacg ac
//
LOCUS       DATAIR010000756         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_359155, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000756 DATAIR010000000
VERSION     DATAIR010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359155"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VHM69_18025"
     CDS             <1..773
                     /locus_tag="VHM69_18025"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2742343.1"
                     /translation="EGFDLGSLDPVSADRIHLEVEAKKLAFKDLHERVGDPEFSEMPV
                     EEMLSKEYAAGLRESISRDAAAPDSPDFGSDTTYLCAVDAEGNGCSFINSLYDRFGSG
                     IVAEGTGVCLHNRGKSFRLIPGHPNAIVPGKRPMHTIIPALATRDGDLWAVFGVMGAA
                     MQPQGHAQLLINLLDYGMDPQSAVDHPRHRHENGVLRIEGRVPENEIARLRGLGHRIE
                     VGADYMIPTGGAQLIRILENGVRACGSDPRKDGCALAQ"
     gene            784..1257
                     /locus_tag="VHM69_18030"
     CDS             784..1257
                     /locus_tag="VHM69_18030"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEX2742344.1"
                     /translation="MTSGQSPARRVAIAGVVGALYVVLSLLVAPLAYGPLQFRLGEVL
                     KPLVIKYPATIPAFAIGVGIVNLFSPQAGGLELILMPVVNLIGGTLCWFVARRIGGVV
                     GTYIASLLFALIIAAGVATVLHFAAGLPYLLAFGSVAVSEVVLLLLGNAVLVRRV"
     gene            1326..1718
                     /gene="queF"
                     /locus_tag="VHM69_18035"
     CDS             1326..1718
                     /gene="queF"
                     /locus_tag="VHM69_18035"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX2742345.1"
                     /translation="MREEQVLGRDVRGPIGAEGLDAVPWSHGDTDAVVEFTTNELTAL
                     CPITGQPDFYELKLDYRPKASLIESKSLKLYLWGFRDRGIFAEDLAATLLKDLVAACD
                     PDEMTIDLTQQVRGGLQIRTVVRHSSKG"
     gene            1859..3136
                     /locus_tag="VHM69_18040"
     CDS             1859..3136
                     /locus_tag="VHM69_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2742346.1"
                     /translation="MANVTEGTAGEATSTWGRRLRLVGPGIIIAVTGVGAGDMISSLV
                     AGTSFGTVLIWAIVIGALMKFALTEGIGRWYMASGETILKGWHMLGRGATWYFLVYLF
                     IVTFVFGAAVTSTAALGADAMFPNLLPVWAWAALHGILGFVVVGIGRYDLFERIMKSI
                     AAIKFGAIILLAILVTPSLTDLALGLVPRIPEGALINVLAIIGGVGGTYTLASYTYWV
                     RERGWRHVSWIPMMRTDAAVGYILTGLFMISMLIIGAEFLFGTGKSIEDEAGILALAN
                     PIQDRFGIFVRWVFLIGFWAVATGAMIGAWNGAAYLFADSIRTFRDVPEEEAEEYLSE
                     KSVWFRAMLVWITFPPMILYAFGEPVALVIVYASLGALFLPFLAITLLLLLNSRRVAP
                     EYRNHLVTNILLAISVIPFIVLGVQEVLGALGG"
     gene            3226..>3626
                     /locus_tag="VHM69_18045"
     CDS             3226..>3626
                     /locus_tag="VHM69_18045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2742347.1"
                     /translation="MRSRPGSSEAAGPGRPLKRVYTILVAFGSIVWFALGVEWFRGMR
                     GISLLSEVGSTGNLERYPSLSVIVPARNEERSINESVETMLSQSYPGELEVIVIDDRS
                     TDGTERILRTLQRANPDRLKLLRVHELPEGW"
BASE COUNT          628 a         1144 c         1152 g          702 t
ORIGIN      
        1 tggaagggtt cgatctcggc tctctagacc cggtgagcgc tgatcgcatc cacctcgagg
       61 tcgaggcgaa gaagctcgcc ttcaaggacc tgcacgagcg ggtcggagac ccggagttct
      121 ccgagatgcc ggtcgaagag atgctatcca aggaatacgc tgctgggctg cgcgagtcga
      181 tctcccgcga cgcagcggcg ccggactctc ccgacttcgg gtccgacacc acctatctct
      241 gcgccgtgga cgccgaggga aacggctgct cgttcataaa cagcctctac gatcgcttcg
      301 gcagcggcat cgtcgccgaa ggcacgggcg tctgcctcca caatcgcgga aaatccttcc
      361 gtctcattcc cggccacccc aacgccatcg tccctggaaa acgcccgatg catacgataa
      421 tccccgctct agctacccgc gacggagacc tctgggccgt cttcggggtg atgggggctg
      481 cgatgcagcc gcagggacac gcgcagttgc tgatcaacct cctcgactac ggcatggacc
      541 cgcaatcggc cgtcgaccac ccccgccacc gtcacgagaa cggcgtcctg cgtatcgagg
      601 gccgcgtgcc tgagaacgag atagcgcgct tgcgcgggct gggccaccgc atcgaggtgg
      661 gggctgacta tatgatcccc acaggaggcg cccaactcat ccgaatcctg gagaacggcg
      721 tcagggcgtg cggcagcgac ccgcgcaaag acggctgcgc gctcgcgcag tagagaggaa
      781 gcgatgacgt caggacagag tccagccaga agggtcgcga tcgcaggagt ggtcggggcg
      841 ctctacgtgg tcctgagcct gctcgttgcg cctctcgcct acgggccgct ccagttcagg
      901 ctaggtgagg tgttgaagcc gctcgtcatc aagtaccccg ccaccatccc agccttcgct
      961 attggggtcg ggatcgttaa cctcttctcc ccgcaggccg gcggactgga gttgatcctg
     1021 atgcctgtag tgaacctgat cggcggtacg ctctgctggt tcgtggcgcg ccgaatcggc
     1081 ggcgtagtcg gcacgtacat agccagcctg ctcttcgccc tcataatcgc cgccggtgtc
     1141 gcaacggtgc tccacttcgc cgccggactc ccgtacctcc tcgcgttcgg gtccgtcgcc
     1201 gtctccgaag tcgtgttgct cctgctcggc aacgctgtgc tggtacggcg ggtctagaag
     1261 ccgctgtagc aggtgaacgc gttgcggtaa aatctgcgtc ctacggaaga tattcggggg
     1321 ttgtgatgcg agaagagcaa gtactgggcc gcgacgtgcg gggtcctatc ggggccgaag
     1381 gactcgacgc tgtgccgtgg agccacggag ataccgacgc ggtggtcgag ttcacgacca
     1441 acgagctgac ggcgctctgc ccgatcacag gccagcctga cttctacgag ctgaagctcg
     1501 actaccgccc gaaggcgtcc ctgatcgaat caaaatccct gaagctctac ctctggggct
     1561 tccgcgacag gggaatcttc gccgaggacc tcgcagccac gctcctgaaa gacctcgtcg
     1621 ctgcgtgcga tcctgacgag atgacgatag acctgactca gcaggtacgc ggcgggctcc
     1681 agatccgcac cgtcgtccgc cacagttcta agggataagc tactccatag cggctgcggg
     1741 ttacttctcg cggaccaacc atcctcgtca acctgcgcgc tgtgcctctt tgtttgccca
     1801 aaaggaacat tgttgtctaa tatggagtaa cgtctggcga cgaaggggga gggaccggat
     1861 ggcaaacgtt accgagggga ctgctggaga ggccacgtct acgtggggac gtcggctcag
     1921 gttagtaggg ccgggcataa tcatcgcggt gaccggggtt ggtgcggggg atatgatctc
     1981 ctcgctcgtg gccggcacga gcttcgggac ggtcctgatc tgggcgatcg tgatcggcgc
     2041 gctcatgaag ttcgccctga cggaaggcat cggccgctgg tacatggcct caggcgagac
     2101 catcctgaaa ggctggcaca tgctcggccg gggcgcgacc tggtacttcc tggtctacct
     2161 gttcatcgtg accttcgtgt tcggggcggc ggtgacctcg acggcggctc tgggcgccga
     2221 cgccatgttc ccgaacttgc tgccggtgtg ggcctgggcc gcgctgcacg gcatcctggg
     2281 tttcgtggtc gtcggcatcg gccgctacga cctgttcgag cggatcatga agagcatcgc
     2341 agctatcaag ttcggggcga tcatacttct cgccatcctg gtgacgccgt cccttaccga
     2401 cctggccctc gggctcgtcc ccaggatacc ggaaggtgcg ctcatcaacg tgttggcgat
     2461 catcggtggg gtcggcggta cgtacacgct cgcctcctac acgtactggg tgcgcgagcg
     2521 cggctggcga cacgtgtcct ggatcccgat gatgcgcacc gacgcggccg tgggctacat
     2581 cctgacagga ctgttcatga tttcgatgct catcatcggg gccgagttcc tgttcgggac
     2641 gggcaagagc atcgaggacg aggcgggcat cctggcgcta gccaacccga tccaggaccg
     2701 cttcgggatc ttcgtccgct gggtcttcct catcggcttc tgggccgtcg cgacaggggc
     2761 catgatcgga gcctggaacg gcgctgccta cctcttcgcc gatagcattc ggaccttccg
     2821 cgacgtgccg gaggaggagg ccgaagaata cctctccgag aagagcgtct ggttccgggc
     2881 gatgctggtc tggatcacct tcccacccat gatcctctac gccttcgggg agcctgtggc
     2941 gctcgtgatc gtctacgctt ccctcggcgc tctgttcctg ccgttcctcg cgatcacgct
     3001 cctgctcctc ctcaactccc gccgcgtggc ccccgagtac cgcaaccacc tcgtcacgaa
     3061 catcttgctg gcgatctccg taataccctt catcgtgctc ggcgtacagg aggtgctcgg
     3121 cgccctgggc ggctaggaga ggttgtttaa tgcgctgctg gatgatcgtc ggactcgagt
     3181 tccgcaccgt cgtccgtcat ccgcgcgaca caggatgact tcgtcgtgag gtctcgcccc
     3241 ggctcatcgg aggctgccgg tccgggacga cccctgaaac gcgtgtacac gattctggtc
     3301 gcattcggct ccatagtgtg gttcgcgctc ggcgtcgagt ggttccgagg aatgaggggc
     3361 atatcgctgc tcagtgaggt cggtagcacg ggcaacctcg aacgctatcc gtcgctctcg
     3421 gtgatagtgc cagcccgaaa tgaggagaga tccatcaatg agagcgtcga gacgatgttg
     3481 tcccagagtt atccggggga actcgaggtc atcgtcatcg acgaccgctc cacggatgga
     3541 acggagagaa tcctccggac gctgcaacgg gcgaacccgg acaggttgaa gctcctccgg
     3601 gtacacgagc tgcccgaagg ctggtt
//
LOCUS       DATAIR010000757         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_359163, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000757 DATAIR010000000
VERSION     DATAIR010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359163"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..607
                     /locus_tag="VHM69_18050"
     CDS             383..607
                     /locus_tag="VHM69_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742348.1"
                     /translation="MERTEEVESGMIARIAGTVIVSAIVLVVVSLLVSVGVYRATDGR
                     ANGALIGIICGAAAAFTVGAVGTVVERSSR"
     gene            656..1231
                     /locus_tag="VHM69_18055"
     CDS             656..1231
                     /locus_tag="VHM69_18055"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX2742349.1"
                     /translation="MSSQGSYRQRLSAFAANWRVLALRGLAALIFGLVVLLWPGAVLA
                     VISLLFGIYAIVDGGVILVPALRTSGRGARRWLPLIEGAVGVIAGLLALLWPGLTASG
                     VLYVIIGWAIATGILKIVTAIMLRSEVENAWLLAGSGALSVLLGVVLAALVGSNVQSL
                     APFIGVFAVVVGIGLIVFAFRTRDRQRARQA"
     gene            1415..>1810
                     /locus_tag="VHM69_18060"
     CDS             1415..>1810
                     /locus_tag="VHM69_18060"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX2742350.1"
                     /translation="MAELGKRSIRNFFEHGMTTYAEQLSYRGLFGLFPFIILVFALLA
                     VLQLDAVFDRLIEAAVGAPTQQVPEPLEPVAQEGRAQATFLRPLIEQAREQAGGGLLS
                     FGIVVSLWSVSAVAFTLTEALNTAYGVEET"
BASE COUNT          346 a          491 c          603 g          370 t
ORIGIN      
        1 tgacgtcgcc gagtacaccc gagatcagca aggaatcccc gcccagcggc tgagcgaagg
       61 agagacgatt ccccgtgact aggtggagga gcacggaggc ttggctgggt gaggccttcg
      121 ccgaactcca atatctagaa gagctttttt ggggcggtca cctgattggc tgctccaggg
      181 cgaagattgt cacgaacgga taggtgcctt tcaggtcggg agcttcacta gcgtaggact
      241 agaacccaat cagcgggaga cctctttgga agaggaacca gaaatagcta gggaactcct
      301 ccagagccat cgaacacaac ccaacggaca agcctggctc ggccgcggag agtccagaaa
      361 tagtcttgat catcttgggg tgatggagcg cacggaggaa gtggaatctg gaatgatagc
      421 caggatcgca gggaccgtga tcgtctccgc aatagttttg gtcgtcgtgt ctttgctcgt
      481 gagcgtgggt gtgtatcggg cgaccgatgg ccgcgccaat ggcgctttga tcgggattat
      541 ctgcggtgcg gcggcggctt tcactgtcgg agccgttggc acagtggtgg agagatcaag
      601 tcgttgagca aagtatgaag agacagtcga gagcacactc gaggaggacg taaacatgag
      661 cagccaggga agctacaggc agaggttgag cgcgtttgcc gccaactggc gggttctggc
      721 gctaaggggg ctcgccgccc tgatatttgg tctcgtggtc ctcttgtggc cgggtgccgt
      781 cctcgctgtg atctctctgc tctttggcat ctacgccatt gtggatggag gggtcatact
      841 cgtgcccgcg ctcagaacgt ccgggcgagg tgcgcgaagg tggctccctc tgatcgaagg
      901 agcggtaggc gtcatcgccg ggctgcttgc ccttctctgg cccggactca ccgcaagcgg
      961 agtgctctat gtcatcatag ggtgggctat cgcgaccggc atcctcaaga ttgtcaccgc
     1021 gatcatgctg cgcagcgagg tagagaacgc gtggctactc gccggcagtg gcgcgttgtc
     1081 ggtgctcctc ggcgtagtcc ttgcggccct ggtgggctct aatgtgcaat ccctggcgcc
     1141 attcatcggc gtcttcgcgg tagtggtggg catagggcta atcgtcttcg ccttcaggac
     1201 acgggaccgg cagcgggccc ggcaggcctg accccaacga gccagagctg ctgcagaaca
     1261 gtaactttaa gcatatgttc acgacgttat gggtgtccaa agagaggcag ataccaggtc
     1321 gtgatcttgc cagtttgcgc cggtcttacg gtcaacgcgg tgcccgccgc gtcgaggact
     1381 aagggtgcgg gttccgggac cacgaggact tgagatggct gagttgggca agcgctcgat
     1441 caggaacttc ttcgagcatg gcatgacgac ctacgcggaa cagctatcct accggggtct
     1501 cttcgggctc ttcccgttca tcatcctggt attcgcgttg ctggcggtac tccagctcga
     1561 cgcggttttc gaccggctaa tagaagcagc cgtgggtgcg ccgacgcagc aagtcccaga
     1621 accgctagag cccgtagccc aagagggcag ggcccaggcg acattcttgc ggcccctgat
     1681 cgagcaggcg cgggagcagg cagggggagg attgctctcc ttcggcatcg tcgtgtcatt
     1741 gtggtcggtc tccgcggtag ccttcaccct gacggaggcc ctgaacaccg cttatggggt
     1801 cgaggagacg
//
LOCUS       DATAIR010000758         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_267590, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000758 DATAIR010000000
VERSION     DATAIR010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267590"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHM69_18065"
     CDS             <1..193
                     /locus_tag="VHM69_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2742351.1"
                     /translation="PRDKEVVAYCRGPYCVFSDEVVRALSARGYRASRLADGFPEWRA
                     AGYPVESGVPISATGEKRA"
     gene            190..1299
                     /locus_tag="VHM69_18070"
     CDS             190..1299
                     /locus_tag="VHM69_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2742352.1"
                     /translation="MTHTSIDVESLREMLERGEPVTVLDVRPEDQRSDWWIPGSVHFD
                     AYGALKAKDPNAMKEAELPEGQPVVTVCAAGNLSAVAAGQLRDQGIETYTLEGGMKAW
                     SMAWNSAEVSVAGTDARVIQVRRTGKGCMSYLICSGGEAAVIDASLDPEVYVALAGSL
                     GWKVTRVLDTHVHADHLSRSRGLAELVGATLHMPESAPVSFPFSAIRDGEAIEVGSAQ
                     LEAVRAPGHTPESTCYLLDGRALLTGDTLFIDSVGRPDLGAKPGVVREKARSLHASLR
                     RLMMMDLGTLILPGHTSTPVPFDEVPVVGSLAEVRKNVPLMRESEEVFVEKISGSVMP
                     PPENYERILELNRAGKLPEGDTTELEAGANRCAVG"
     gene            1306..2556
                     /locus_tag="VHM69_18075"
     CDS             1306..2556
                     /locus_tag="VHM69_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742353.1"
                     /translation="MTAEQEPPPARLGLEQNWRQFALLVLVNAFVGGMVGLERTVVPL
                     VGAQEFGLVLRTAIFSFIVSFGVVKACSNLVSGALADSFSRKKVLVAGWLIGVPVPFM
                     LMLAPSWGWIVAANVLLGVNQGLAWSMTVLMKIDLVGPRGRGLAVGLNEFAGYLAVGL
                     TALATGYLASVYGLRPEPFYLGVAYVVLGLLVSAFLVRDTGEHVRLEMEAHPPEPSPL
                     SFREVFFMTSFKDRNLFACSQAGLINNLNDGMSWGVFPLFFNAFGLGVERIGILKAVY
                     PAVWGILQVATGPLSDRWGRKGLIVTGMWVQAGALFATVLGRSFGWWLLGSVLLGLGT
                     AMVYPSLIAAVSDASRPSWRARSLSVYRFWRDLGYAVGALSAGVISDALGLSWAIGSA
                     GVLTFLSGVVAATSMREGGDGGTK"
BASE COUNT          449 a          844 c          924 g          500 t
ORIGIN      
        1 acccagagac aaggaggtag tcgcctactg ccggggaccc tattgcgtat tctccgacga
       61 agtggtgcgt gctctgagtg cccgaggata ccgggcgagc aggctcgctg atgggttccc
      121 cgagtggcgg gcggcgggat atccggtcga gtcgggggtc ccgatcagcg ctacaggaga
      181 gaagagggca tgacccacac ctctatcgac gtcgagtcct tgagggagat gctggagcgg
      241 ggcgagcctg tcaccgtgct cgacgtgcgc ccggaggacc agcgcagcga ctggtggatc
      301 ccaggcagcg tccacttcga cgcctatggt gccctcaagg cgaaggatcc caacgccatg
      361 aaggaggccg agttgccgga aggacaaccc gtcgtgaccg tctgcgctgc gggcaacctc
      421 agcgctgtgg ccgccgggca gttgagggac caggggatcg agacctacac gctggagggc
      481 gggatgaagg cgtggagtat ggcctggaac tcggcggagg tgagcgtggc tgggacggat
      541 gcgcgggtca tccaggtgag gcgcacgggc aaggggtgca tgtcgtacct gatctgctcc
      601 ggaggcgagg cggctgtgat cgacgcctcc ctcgaccccg aggtgtacgt cgcactggcc
      661 ggctctctgg gttggaaagt aacacgcgtg ctcgacaccc acgtccatgc cgaccacctc
      721 tcccggtcgc gcgggctggc cgagctcgtc ggggccacgc tccacatgcc ggagagtgcg
      781 ccggtttcct tccctttctc cgcgatcagg gacggggaag caatcgaggt cggctcggca
      841 cagctcgagg ccgtcagggc gccgggacat acccctgaga gcacctgcta cctcctggac
      901 ggacgggcac tcctcacagg ggacacgctc ttcatcgact cggtgggccg accggacctg
      961 ggggcgaaac cgggtgtggt acgggagaag gcgcgctccc tgcacgcctc tctgcgtcgc
     1021 ctgatgatga tggacctggg aacgctcatc ctacccggac ataccagcac ccccgtcccc
     1081 tttgacgaag ttcccgtcgt tggcagcctg gcggaggtgc gaaaaaacgt tcctctcatg
     1141 cgagagagcg aggaagtctt cgtcgagaag atttcgggta gtgtcatgcc tccgccggag
     1201 aactacgagc gcatcctgga gttgaacagg gccggaaagc taccggaagg ggataccacc
     1261 gaactggagg ctggtgcaaa ccgctgtgct gttggctaga gttctgtgac cgcagaacag
     1321 gagcccccgc ctgcccggct ggggctcgag cagaactggc ggcagttcgc gctgctggtc
     1381 ctggtcaacg ccttcgtcgg cggcatggtc ggtctggagc gcaccgttgt gccgctcgta
     1441 ggcgcacagg agttcgggct cgtcctcagg acggccatct tctcgttcat cgtctccttc
     1501 ggcgtggtga aggcctgctc gaacctcgtc tcaggggcac tggccgattc cttcagcagg
     1561 aagaaagtac tggtggctgg gtggctgatc ggtgttcccg taccgttcat gctcatgctc
     1621 gcgccctcgt ggggttggat cgtggctgca aacgtcctcc tcggggtgaa ccagggtctc
     1681 gcatggtcca tgacggtcct gatgaagata gacctcgttg gaccacgggg acggggcctc
     1741 gctgtcggcc tcaacgagtt cgcaggctac cttgcggtcg ggctcacggc gctcgccaca
     1801 gggtacctcg cctcggtcta cggcctcagg cccgagccct tctacctcgg ggtggcttac
     1861 gtcgtccttg ggctcctcgt cagcgcgttc ctcgtgcgcg acaccggaga gcacgtccgg
     1921 ctggagatgg aggcccaccc gccggagccg agcccactca gcttccgcga ggtcttcttc
     1981 atgacctcct tcaaggaccg caacctcttc gcctgctcgc aagccggcct catcaacaac
     2041 ctcaacgacg gcatgagctg gggcgtcttc cccctgttct tcaacgcctt cggcctcggc
     2101 gtggagcgca tcgggatcct caaagcggtc taccctgcgg tatggggaat actgcaggtc
     2161 gcgaccggtc cgctgagcga ccgctggggc cgcaagggcc tcatcgtcac aggcatgtgg
     2221 gtgcaggcgg gcgctctctt cgccaccgtg ttgggcagga gcttcgggtg gtggctatta
     2281 ggaagcgtgt tgcttgggct cgggacggcc atggtctatc cctccctcat cgccgccgtc
     2341 tccgatgcct cgcgcccttc ctggcgggcc aggtccctga gcgtctaccg attctggcgg
     2401 gacctcggct acgccgtcgg ggcgttgtcg gcgggtgtga tctccgacgc cctcggcctg
     2461 tcgtgggcga tagggtcggc cggggtactg accttcctct ccggcgtggt cgccgcgacc
     2521 tcgatgcgag agggagggga tggtggaacc aagtaggcgg tagcaagtag atgaacgcat
     2581 agggagcagc cggattcctg taaggtggca gaagctcatt ccgacgacta cggggcaacc
     2641 ctggaacaat acccgcagcc tccaagtcag gctacaatcc cgtgcgggat cgactcccgc
     2701 tgggcttgtt agataga
//
LOCUS       DATAIR010000759         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_222266, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000759 DATAIR010000000
VERSION     DATAIR010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222266"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VHM69_18080"
     CDS             complement(<1..529)
                     /locus_tag="VHM69_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2742354.1"
                     /translation="MAARAETAQQTRGKRDTGRRTDLTGYLFVLPFLIAYALFLVWPV
                     ILGLRMSFFDWSLVGTGTRDFLGLANFTELFSDPDFWASLWHTILFTLLSTPPLVLLA
                     LGLALLANRAVPARWFFRLAFFAPYVLPVSTVALIWVWLYQPGFGLINSYLVSLGLPE
                     VDWLAGENVAMISVVI"
     gene            complement(563..>1782)
                     /locus_tag="VHM69_18085"
     CDS             complement(563..>1782)
                     /locus_tag="VHM69_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2742355.1"
                     /translation="PFGSSKQTVHFWNLFGGGDGARLADMESDFVKTHPGIDLKATTL
                     TWGAPYYTKVSMATVGGRPPDVAVMHLTRLPAYAPAGLLEPLDPDVLAEHDIGQDKFL
                     PEIWEGGKYKGEIYDIPLDTHPQVMYYNTDICKEAGLLDSDGNLKPLQGREELIDAFQ
                     KTMDVTKEQGIAFAPADGVVPWRLFYSFYGQLGGEVLSPDASEVILDQEKAEEALSFL
                     VELTVDKEFTPSNQDYAAAVAQFQSGNAGFHWNGEWEVTTFLDAKMPFSMAEFPDVFG
                     NKRVQADRHTFVIPKGVANDVKAFDAALQFVSSMLKSSLIWAEGGHIPAYRSVLESSE
                     YKNLQPQSNYAGVADKVVLDPKAWFSGSGSDMEVQASVPMQQAMAGSIEPKEAVEQIR
                     AAMQELVDTPKPF"
BASE COUNT          329 a          566 c          551 g          336 t
ORIGIN      
        1 cgatgaccac ggagatcatg gcgacgttct cgcccgccag ccagtcgacc tcgggcaacc
       61 cgagcgagac gaggtagctg ttgatcaagc cgaacccggg ctgatagagc cagacccaga
      121 tgagcgcgac tgtggacacg ggcagcacgt acggcgcgaa gaaggcgagc cggaagaacc
      181 accgggccgg tactgcccga ttagccagga gcgcgagccc gagagcgagc aggaccagcg
      241 gaggggtact cagcaaggta aataagatcg tgtgccacag ggaggcccag aagtcggggt
      301 cgctgaatag ctccgtgaag ttggcgagtc ccaggaagtc cctcgtgccc gtcccgacca
      361 ggctccagtc gaagaagctc atcctcaggc ccaggatcac gggccacacg aggaagagtg
      421 cgtaggcgat gaggaacggc agaacgaaga ggtaccctgt gagatcggtc ctccgtcctg
      481 tatccctctt ccctctagtc tgctgcgcgg tctcggcccg tgccgccaat cctgtacctc
      541 cccgtagtcc tctggtctgt tgctagaagg gcttgggcgt gtcgacgagc tcctgcatcg
      601 cggcccgtat ctgctctacc gcctctttgg gctcgatgct ccccgccatg gcctgttgca
      661 tcggcaccga agcctgaacc tccatgtccg agccggagcc gctgaaccag gccttggggt
      721 caaggaccac cttgtcggcg accccggcgt agttgctctg cggctggaga ttcttgtact
      781 cggagctttc gagcacggac cggtaagccg ggatatgccc gccctcggcc cagatcaggc
      841 tgctcttgag catggagctg acgaactgga gcgcggcgtc gaacgccttg acatcgttgg
      901 ctaccccctt cgggatgacg aacgtgtggc gatcggcctg gacgcgcttg ttgccgaaca
      961 cgtcggggaa ctcggccatg ctgaagggca tcttggcatc caggaaggtg gtgacctccc
     1021 attctccgtt ccagtggaaa ccggcgttgc cgctctggaa ctgggccacg gcagcggcgt
     1081 agtcctggtt gctcggcgtg aactccttgt ccaccgtgag ctcgaccagg aacgacaggg
     1141 cctcctcggc tttctcctga tccaagatca cttcgctcgc gtcgggcgag agcacctcgc
     1201 ctccgagctg gccgtagaag ctgtagaaca accgccacgg caccacccca tccgctggcg
     1261 cgaacgcgat gccctgctcc ttggtgacat ccatggtctt ttgaaacgcg tcaatgagct
     1321 cttcgcgccc ctgaagcggc ttgaggttgc catcggaatc caggagcccg gcctctttgc
     1381 agatgtcggt gttgtagtac atgacctgcg ggtgggtatc cagcgggatg tcgtagatct
     1441 ctcctttgta cttgccgccc tcccagatct ccggcaagaa cttgtcctgg ccaatgtcgt
     1501 gctccgcgag cacgtcaggg tccagcggct cgagcaaccc agcaggcgcg taggccggta
     1561 gcctggtcag gtgcatcacg gcgacgtccg gtggccgccc accgaccgtg gccatcgaga
     1621 ccttggtgta gtacggagcg ccccacgtta gcgtggtcgc cttgagatcg attccagggt
     1681 gggtctttac gaagtcgctt tccatgtcag caagccgcgc cccgtcacct ccgccaaaca
     1741 ggttccagaa atggaccgtc tgcttcgaac taccaaatgg tc
//
LOCUS       DATAIR010000760         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_267905, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000760 DATAIR010000000
VERSION     DATAIR010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267905"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VHM69_18090"
     CDS             <1..766
                     /locus_tag="VHM69_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742356.1"
                     /translation="ECAMVFQSAQNALNPVMRIRDHFADTARDHAESDRPLKGRALTE
                     RATECLRLVQLDPDRVLKAYPHELSGGMRQRVMIALGLLLNPRVLILDEPTTALDVLT
                     QRAIIEVLRELREGHRFAMIFVSHDLSLAAELADRVATTYAGRIVELGDVEQVFYHPR
                     HPYTVGLLDAVPTLAGEHKELSSIPGSPPDLIDLPTGCKFHPRCPYATDECREEDPPL
                     IEVAPGQMAACWHWEKVGFDWEARGGERHERDANRA"
     gene            741..1793
                     /locus_tag="VHM69_18095"
     CDS             741..1793
                     /locus_tag="VHM69_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742357.1"
                     /translation="MSGTPIELEHVSRFYGSGSQQVRAVDDVSFTIESGETLCLVGES
                     GCGKSTTGRMMAGLLEPSEGRVLYGGSDVSKLPEEEKKRFRRAVQIIHQDPYASLNPI
                     RNVTQFLSAPLKRHKLTKSRSELRTKLHELLETVGLTPPEDFLRKHPHELSGGQRQRV
                     SVARALTVDPEVIVADEPVSMVDVSLRIGLLNMLLTLQRDLGVAFLFITHDLAVAKHF
                     SWDGRIGVMYLGRMVELGSTQRLVKEPVHPYTRALISALPEADPELTRNKERLRLRSP
                     DIPSLLNLPAGCPFHPRCPMYEEGLCDTDRPAEDAVALDGHLAACYVAARELGQEGRI
                     PESSMAAAARANRRTA"
     gene            1790..1972
                     /locus_tag="VHM69_18100"
     CDS             1790..1972
                     /locus_tag="VHM69_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742358.1"
                     /translation="MIPELGKAGFRIGFLIVVPSFFLMLFLDPGTAEYVITVLTLVMG
                     VVFLVAVTLLVLYSRR"
     gene            2010..>3746
                     /locus_tag="VHM69_18105"
     CDS             2010..>3746
                     /locus_tag="VHM69_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2742359.1"
                     /translation="MSTTEGIYRPKALTRRQFLGMSAAGAGLLLAGCGGGGGQNYGEG
                     GGGGAGGAKEFHGAYPYQLPPTGHFNTFATNGIFALTLYQDVLEMPMAMYLWADQKYV
                     PYMATEWGYEPPDVFKVTLREGAKWSDGSEFTSEDVITTFNLLRLQEQLVWNYVGSME
                     ADDEYNLTFTMKDPSTVVERLVLKEPIRATSVYGEWSQKAQELFDSDTDPESKEYRDL
                     VTEFEGFKPEEMIVTGPFKIDKDSITESQLTMTKVDTAWNADQTDLEKMVIYNGETPA
                     VTPLVLSKDIDYATHGFPIATEKSFEDQGIRIIRAPTYSGPALYFNYAKIKAVADPRV
                     RRAIAKAIDMDENGTVSLAKSAKRSEYMTGVSDVLIKDWVSQEDLSKMDSYEYDPDAA
                     ASELEDLGFEKDGDVWVSPEGERMEYELGAPAEFADWSAAAKNLGDQLTKFGIKTTVR
                     TVTFTQWEPRMQDGDFEMGIQAWGQGQPHPVYSFEHPLMTYNVAASGGGMSYDMTQDT
                     ESYGKVDLEKLVIQSASGLNDEDQKATVTKLSTIFNELLPVIPLWERYGNNPALDGER
                     VTGWLPDSSSIYQ"
BASE COUNT          781 a         1123 c         1197 g          645 t
ORIGIN      
        1 ggagtgcgcg atggtgttcc agtcggcgca gaacgcgctc aaccccgtga tgcgtatccg
       61 cgaccacttc gccgacaccg ccagagacca cgctgagtcg gacaggcccc tcaagggccg
      121 cgccctcacg gagcgggcta cagaatgcct gcggctcgtt cagctcgacc cggaccgcgt
      181 gctcaaagcc tacccgcacg agctctcggg aggcatgcgc cagcgggtca tgatcgcgct
      241 cggtctactg ttgaacccac gggtactgat cctggacgaa ccaacgacag ccctggacgt
      301 gctgacccag cgcgccataa tcgaggtgct cagggagttg agggaggggc accggttcgc
      361 catgatcttc gtctcccacg acctgtccct ggccgccgag ctagccgaca gggttgcaac
      421 gacgtacgcg ggacgcatcg ttgagctcgg ggacgtcgag caggtcttct accacccgcg
      481 tcacccctat acggtcggcc tgctggacgc ggtgccgacc ctggcagggg agcataagga
      541 gttgagttcg atccccgggt ccccgcctga tctgatagac ctcccgacgg gctgcaagtt
      601 ccatccgcgg tgcccgtacg ccacggacga atgccgcgaa gaggacccgc cgctgatcga
      661 ggtggctccc ggacagatgg ccgcgtgctg gcactgggag aaggtaggtt tcgactggga
      721 agccagggga ggtgagcgcc atgagcggga cgccaataga gcttgagcac gtgagccggt
      781 tttacggttc gggcagccag caggtgcggg cggtggacga cgtatcgttc accatagagt
      841 caggcgagac cctgtgtctc gtcggcgaga gcgggtgcgg caaatccacg acgggacgca
      901 tgatggcggg cctgctcgaa ccctcggaag gtcgcgtgct ctacggcggc agcgatgtca
      961 gcaagctccc ggaggaagag aaaaagcgct tccgtcgagc ggtccagatc atccaccagg
     1021 acccgtacgc ttcgttgaac cctatacgca acgtgacgca gttcctgagc gcccctctaa
     1081 agcgccacaa gctcacaaag tcgcgctccg agctcagaac caagctccac gagctgctgg
     1141 agacggtcgg actgacgccg ccggaggact tcctgagaaa gcaccctcac gagctttcgg
     1201 gcgggcagcg ccagagggtc tccgtcgcgc gggcgctcac cgtagacccg gaggtcatag
     1261 tggccgacga gccggtctcg atggtcgacg tctcgttgcg tatagggttg ctcaatatgc
     1321 tgctgacttt gcagcgggac ctcggcgtgg ctttcctctt catcacgcac gacctcgccg
     1381 tcgccaagca cttctcgtgg gacgggcgga tcggcgttat gtatctgggg cgtatggtcg
     1441 agcttggctc cacgcagcgg ctcgtcaagg agccggtgca tccctatacg cgggccctga
     1501 tctccgccct gcccgaggca gacccagagc tgacgcgcaa taaggagagg ctgcgactga
     1561 gaagcccgga catcccgagc ctgctgaact tgccggcggg ttgcccgttc catccccgct
     1621 gcccgatgta tgaagagggg ctctgcgaca cggaccgtcc ggccgaggat gccgtagccc
     1681 tcgacgggca cctcgcggct tgctacgtcg ccgccagaga gcttggccag gaggggcgca
     1741 tacccgagag cagcatggcg gctgccgcca gggccaatcg gaggacggcg tgatccctga
     1801 acttggaaaa gcgggattca ggatagggtt ccttatcgta gtgccgtctt tcttcctgat
     1861 gctcttccta gaccccggga ccgcggagta tgtgatcact gtccttaccc tggtgatggg
     1921 ggtggtcttt cttgttgctg taacgctgct ggttctgtac agcagacgct agacacgtac
     1981 ggagactctg gcgataaagg aggcaatgaa tgagtacgac cgagggaatc taccgtccaa
     2041 aggcattgac ccggcggcag tttctcggca tgagcgccgc gggggccgga ctgttgctcg
     2101 ccggctgcgg cggcggtggt ggccagaatt acggtgaagg tggaggcggt ggggcaggag
     2161 gtgccaagga gttccacggc gcctatccct accagctacc tccgaccggg catttcaaca
     2221 cgttcgccac caacggaatc ttcgcgctga ccctgtatca ggacgttctt gagatgccca
     2281 tggcgatgta tttgtgggcc gatcagaagt acgtcccgta tatggccacg gagtgggggt
     2341 acgagccgcc tgacgttttc aaggtgacgc tgcgcgaggg cgcgaaatgg agcgacggct
     2401 cggagtttac ttctgaggac gtgatcacca ccttcaacct gcttcgcctg caggagcaac
     2461 tggtgtggaa ctacgtcggg tcaatggagg ccgacgacga atacaacctg acgttcacca
     2521 tgaaggaccc atcaacggtc gtcgagcggc tggtgctcaa ggagcccatc agggcgacct
     2581 ccgtttacgg tgaatggtct cagaaagccc aggagctctt cgatagcgac accgaccccg
     2641 agtccaagga gtacagggac ctggtcacgg agttcgaggg attcaagccg gaagagatga
     2701 tcgtcaccgg cccgttcaag atagataagg acagcataac cgaatcgcag ctcacgatga
     2761 ccaaagtaga caccgcgtgg aacgctgacc agacggacct cgagaagatg gtcatataca
     2821 acggcgagac ccctgcggtc acaccactgg tgctctccaa ggacatagac tacgccacgc
     2881 acgggttccc aatcgcaacc gagaagtctt tcgaggacca ggggatcagg atcatccgcg
     2941 cacccaccta ctccggaccg gcgctctact tcaactacgc caagatcaaa gccgtcgcag
     3001 acccgagggt gaggcgggcc atcgcgaaag ccatagatat ggatgagaac ggcacagtct
     3061 cgctggccaa gtccgccaag agatcagagt acatgacggg cgtttcggac gtcctgatca
     3121 aggattgggt ctcgcaagaa gacctgtcga aaatggactc atacgagtac gatcctgacg
     3181 cagccgccag cgagctggag gacctcggtt tcgagaagga cggcgacgtc tgggtgtcgc
     3241 ccgaagggga gaggatggag tacgagctcg gcgcccccgc ggagttcgcc gactggtcgg
     3301 cggcggccaa gaatctcgga gaccaactaa cgaaattcgg aatcaagacg accgtgagga
     3361 cggtcacctt cacccagtgg gaacccagga tgcaagacgg ggacttcgag atgggcatcc
     3421 aggcgtgggg ccagggacag cctcaccccg tctactcgtt cgagcacccc ctgatgacgt
     3481 ataacgtggc cgcatcgggt ggcggcatgt cttacgatat gacgcaggac acggagtctt
     3541 acggaaaggt tgacctggag aagctggtca tccagagcgc ctccggcctc aacgatgagg
     3601 accagaaagc gaccgtgacc aaactgtcca cgatcttcaa cgagctctta ccggtcatcc
     3661 cgctctggga gcgctacggc aacaacccgg cactcgatgg tgaacgggtt acgggctggt
     3721 tgccagatag cagctccatc taccag
//
LOCUS       DATAIR010000761         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_360401, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000761 DATAIR010000000
VERSION     DATAIR010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360401"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2889
                     /gene="gltB"
                     /locus_tag="VHM69_18110"
     CDS             <1..2889
                     /gene="gltB"
                     /locus_tag="VHM69_18110"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX2742360.1"
                     /translation="MSLGPEQNLFDETPEHCRRVLIDQPVLTADELEKIRRLGSERAA
                     TSTLSTLFPVASGEEGMEQALSRLCGKAESAVKGGSAVLVLSDRGVNEAQAPIPSLLA
                     TASVHHHLVRAGIRTATTLVVETAEAREVHHFALLVGYGATAVNPYLAFETIEELAGS
                     HLMEGVSPEEAAKNYVKAVGKGLLKVISKMGISTLFSYCGAQIFEAVGLNQEIIDRCF
                     TGTASRVGGVGMDALARETLERHRNAFYAVEDNADELEIGGEYQLRAQGQYHQWNQLS
                     IPPLQRAVKTGSFETFKEFTRHFDEHSARFANLRGLFDFQENPIPIEEVEPAKEIVKR
                     FVTGAMSFGSISKEAHETLAIAMNRIGGKSNTGEGGEDPTRFIPDPNGDNRRSAVKQV
                     ASGRFGVTTEYLVNADVLQIKMAQGSKPGEGGQLPGHKVSEDIAKVRYSTPGVGLISP
                     PPHHDIYSIEDLAQLIHDLKNANPRADVSVKLVAEVGVGTVAAGVAKAKADHITISGH
                     DGGTGASPLSSIKHAGLPWELGLAETQQVLVQNDLRGRVILQTDGQLKTGRDVVVAAL
                     LGGEEFAFSTAPLVATGCIMMRVCHLNTCPVGIATQDPELRKRFTGTPEHVVNYFFFL
                     AEEVRELMAGMGFRTFEEMVGRSDMLKMREAIEHWKARGVDLSAILHRPDAPEGAAIS
                     HSERQDHRLDKSMDNELIEKCMPALENGEEVRFEHQITNTSRTVGGMLSGEVARRYGN
                     DGLPDGTVRIDFHGVAGQSFGAWLARGLTFTLEGTTNDYVGKGLSGGRLAVFPSRLAA
                     YKPTRNVVVGNVALYGATGGEAYFRGFAGERFAVRNSGAHTVVEGVGDHGCEYMTGGI
                     VVVLGETGRNFAAGMSGGVAFVLDEEERFDTLCNMDMVGLEAVESEEDIDLLHGMVER
                     HLEWTGSERARRVLKEWDEVLPRFVKVMPHDLKRVLRERQEAELEVAS"
     gene            2889..4316
                     /locus_tag="VHM69_18115"
     CDS             2889..4316
                     /locus_tag="VHM69_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX2742361.1"
                     /translation="MGDPKGFLKIGRKPTPKRTVEERVQDYRYVYEPMPDEELKGQAS
                     RCMDCGVPFCNTGCPLGNLIPDWNDLVYREHWKQAIDRLHQTNNFPEFTGMLCPAPCE
                     TACVLAINDKPVTIKEIELSIINRAFEEGWVTARPTPPEKRTGKKVAIVGSGPAGLAA
                     AQQLNWAGHTAVVFEKDDKIGGLLRYGIPDYKIEKWVVDRRIAIMEEEGVEFRTNAHV
                     GEDPTTEELKSEFDAIVLAVGALQGRDLDVPGRELGGIHLAMDYLVQQNRRVAGLPTN
                     AEVEISAKGKNVVILGGGDTSADCLGNAHREGCASVRVLTHGPRPPENPDHLTWPDWP
                     LILHTYPAHEEGGERGFSVAVKGFSGSNGHVERMRAVETERTNGKTVPKEGTDFEIET
                     DLVLLAIGFTGPVKDRFMQEMDLGYTDRGAIHSENGFGTNEPGTFVAGDAKRGASLIV
                     WAIAEGRKAARQADEYLMGRSLLPG"
     gene            4488..4976
                     /locus_tag="VHM69_18120"
     CDS             4488..4976
                     /locus_tag="VHM69_18120"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2742362.1"
                     /translation="MDGKGNPIPDGFHTVTPYVTIKGAADAIKGAADAIGFYQEAFGA
                     REIFRWADPDGRIRHAEVVIGDSPVMLTDEAPEFGMSGPQSLGGSPVHMFLYVEDADA
                     VFNRAISAGATELMPVEDSSDGDRRGGVTDPFGHVWYIGTHVEDISREELQKRYDEMT
                     RQ"
     gene            5135..>5594
                     /locus_tag="VHM69_18125"
     CDS             5135..>5594
                     /locus_tag="VHM69_18125"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2742363.1"
                     /translation="MGRIEFSRESARELLLAAQGIGSWPERPATKEDVLGAVRKMGVL
                     QIDSISVVARSPYLVLWSRLGSYEPLWLDELLAEGALFEYWSHAACFIPIEDYGLYRG
                     LMLGDRENPVAWMQAHPDAIAHVMEYVRENGSVRSAEFRRADGRAGGWWDW"
BASE COUNT         1102 a         1735 c         1875 g          882 t
ORIGIN      
        1 atgagcctcg ggcccgagca gaacctgttt gacgagacgc ctgagcactg ccggcgcgtc
       61 ctcatagatc aacccgttct aaccgccgat gagctcgaga agatccggcg cctcggctcc
      121 gaaagggcgg ccaccagcac gctctcgacc ctcttccccg tcgcctctgg cgaagagggg
      181 atggagcaag ccctgagccg cctgtgcggg aaggccgaga gcgcggtgaa gggcggttcg
      241 gcggtcctgg tcttgagcga caggggcgtg aacgaggcgc aggccccgat ccctagcctc
      301 cttgctaccg cgagcgttca ccaccatctg gtgcgggcag gtatcaggac ggcgacgacg
      361 ctggtcgtgg agaccgcgga ggcgcgtgag gtccaccact tcgccctcct cgtagggtac
      421 ggcgcgaccg ccgtgaaccc atacctcgcc ttcgagacca tagaagagct cgcaggctcg
      481 cacctcatgg agggcgtgag ccccgaggag gccgccaaga actacgtcaa ggccgtgggc
      541 aaaggcttgc tcaaggtcat ttccaagatg ggcatctcga cgctgttttc gtactgcggg
      601 gcccagatct tcgaggcagt cgggttgaat caggagatca tagacaggtg cttcacgggc
      661 acggcctcgc gcgtcggcgg cgtgggaatg gacgctctgg cgcgcgagac gctagagcga
      721 cacaggaacg ctttctacgc ggtcgaggac aacgcggatg agctcgagat cgggggcgag
      781 taccagctca gggcgcaggg acagtaccat cagtggaacc agttgagcat ccctcccctc
      841 cagcgcgccg tcaagacggg gagcttcgag accttcaagg agttcacgag gcacttcgac
      901 gagcacagcg cgcgcttcgc caacctgcgc ggcctcttcg atttccagga gaaccccatt
      961 cccatagagg aggtcgagcc cgcgaaggag atcgtcaagc gcttcgtcac gggcgcgatg
     1021 tctttcggct ccatctcgaa ggaggcccac gagacgctcg ccatcgccat gaaccgcatc
     1081 ggcgggaagt ccaataccgg agagggcggc gaggacccga cgcgcttcat acccgacccg
     1141 aacggcgaca accgccgcag cgccgtcaaa caggtcgcga gcgggcgctt cggtgtgacc
     1201 acggagtacc tcgtcaacgc ggacgtattg cagatcaaaa tggcccaggg gtcgaagccg
     1261 ggcgagggcg gccagctccc gggccataaa gtttcagagg acatagccaa ggtccgctac
     1321 tcgacacccg gggtcggcct gatctcaccc cctccgcacc acgatatcta ctccatcgag
     1381 gacctcgcgc aactcatcca cgacctgaag aacgcgaacc cccgcgccga cgtgagcgtg
     1441 aagctcgtcg ccgaggtcgg ggtcgggacg gtcgcggcag gcgtcgcgaa ggcgaaggcc
     1501 gaccacatca ccatctcagg ccacgatgga gggacaggag cttctccgct ctcctccata
     1561 aaacacgccg gattgccgtg ggagctcggg ctcgcggaga cgcagcaggt gctcgtccag
     1621 aacgacctgc gagggcgagt gatcctccag accgacgggc aactcaagac gggccgcgac
     1681 gtggttgtgg ccgcccttct cggcggcgag gagttcgcgt tctcgacggc tccgctggtt
     1741 gcgacaggct gcatcatgat gcgcgtctgt cacctgaaca cctgtccggt cggcatcgcg
     1801 acgcaggacc ccgagctgag gaagcgtttc acgggcacgc cggagcacgt cgtcaactac
     1861 ttcttcttcc tcgccgagga ggtgagggag ctgatggcag ggatgggatt ccggaccttc
     1921 gaggagatgg tcggacgctc ggacatgctg aagatgcgcg aggccataga gcactggaag
     1981 gcccgcggcg tcgacctctc tgccatcctg caccgccctg acgcgccgga gggtgcggcc
     2041 atcagccact ccgagcggca ggaccacagg ctcgataagt ccatggacaa cgagttgatc
     2101 gagaagtgca tgccggccct cgaaaacggc gaggaggtcc ggttcgagca ccagatcacg
     2161 aacaccagcc gtaccgtcgg cgggatgctc tcgggtgagg tagcccgtcg ctacggcaac
     2221 gacggcctgc ccgacggcac tgtccgcata gacttccacg gcgtcgcggg ccagtccttc
     2281 ggcgcatggc tcgcgcgcgg cctgaccttc accctcgaag gcacgaccaa cgactacgtc
     2341 ggcaagggcc tatcgggtgg acggctcgcc gtcttccctt cccggctcgc tgcatacaag
     2401 ccgaccagga acgtcgtcgt cggcaacgtg gcgctctacg gggcgacggg cggggaggcg
     2461 tacttcaggg gcttcgcagg ggagcgcttc gcggtcagga actcgggcgc ccacaccgtc
     2521 gtcgagggcg tcggtgacca cggctgcgag tacatgacgg gcgggatcgt cgtcgtgctc
     2581 ggcgagaccg ggcgcaactt cgccgcaggc atgagcggcg gcgttgcgtt cgtgctggac
     2641 gaggaggagc gcttcgacac gttgtgcaat atggacatgg tcggactcga ggccgtcgag
     2701 agcgaggagg acatcgatct ccttcacggt atggtcgagc ggcacctgga gtggaccggg
     2761 agcgagcggg cgcggcgcgt cctgaaagag tgggacgagg tgctgcccag gttcgtgaag
     2821 gtgatgccgc acgacctcaa gagggtgctc agggaacggc aagaagccga gctggaggtg
     2881 gcgagctagt gggagacccg aagggcttcc tcaagatcgg gcgcaagccc accccgaagc
     2941 ggaccgtcga ggagcgtgtc caggactacc gctacgtcta cgagccgatg cctgacgagg
     3001 agttgaaagg ccaggcctcg cggtgcatgg actgcggcgt gcctttctgc aacacgggct
     3061 gtcccctcgg caacctcatc cccgactgga acgacctcgt ctaccgcgag cactggaaac
     3121 aggctataga ccgcctgcac cagaccaaca acttccccga gttcacaggg atgctctgcc
     3181 ccgcgccctg cgagaccgcg tgcgtcctcg ccatcaacga caagcccgtc acgatcaagg
     3241 agatagaact ctccatcatc aacagggcgt tcgaggaagg atgggtcaca gcaaggccaa
     3301 ctccgcctga gaagcggaca ggcaagaagg tcgccatcgt cgggagcggg cctgcggggc
     3361 tcgccgcggc gcagcagctc aactgggcag ggcacaccgc cgtcgtcttc gagaaggacg
     3421 ataagatcgg cggcctcctc aggtacggca tcccagacta caagatcgaa aagtgggtag
     3481 ttgaccgccg catcgccatc atggaagagg aaggcgtcga gttccgcacc aacgcccacg
     3541 tcggcgagga cccgaccacg gaggaactga agagcgagtt cgacgccatc gtcctcgccg
     3601 tcggcgccct gcagggacgc gacctcgacg tgccaggacg tgagctcggc ggcattcatc
     3661 tggcgatgga ctacctcgtt cagcagaacc ggagggtggc cgggctcccg acgaacgctg
     3721 aggtggagat ctccgcgaag ggcaagaacg tcgtcatcct cggtggaggc gacacgagcg
     3781 ccgactgcct cgggaacgcc caccgcgagg gctgcgcctc ggtgagggta ctgacccacg
     3841 gcccccgtcc acccgagaac cccgaccacc tcacctggcc agactggcct ttgatcctgc
     3901 acacctaccc ggcgcacgag gagggcggcg agcgcggctt ctccgtcgcc gtcaagggct
     3961 tctccgggag caatggccac gtcgagagga tgcgcgccgt cgagaccgag cgcacgaacg
     4021 gcaagaccgt ccccaaggag ggtacggact tcgagataga gaccgacctc gtgctcctcg
     4081 ccatcggctt cacaggcccc gtgaaggacc gcttcatgca ggagatggac ctcgggtaca
     4141 ccgaccgcgg agccatacac tccgagaacg gattcggcac gaatgagcct ggcaccttcg
     4201 tagcgggcga cgccaaacgt ggcgcctccc tcatcgtgtg ggcaatcgcc gagggccgca
     4261 aggccgcgag gcaggccgac gagtatctca tggggcgcag tctgctgcct ggataggttt
     4321 caggttcata ggcttcaggt ttcaggtatc aggcttcagc caaaacctta gacctgtggc
     4381 acaagggcgg gactcatcgc cgagtctgtt ctagatccgg ggatgggaac cggatgatcc
     4441 ttgcgtacac tcaagctact gatgacgtgg gcttgggaag ggatcgaatg gacggcaaag
     4501 gcaaccccat acctgatggt tttcacactg tcacgcctta cgtgacgatc aaaggcgcgg
     4561 cggatgcgat caaaggcgcg gcggatgcga tcgggttcta ccaggaagcg ttcggagcca
     4621 gggagatctt ccgctgggct gatccggacg gaagaatccg gcacgctgag gtcgtgatcg
     4681 gcgactcgcc ggtcatgttg accgatgagg ctcccgagtt cggaatgagc ggtccgcaat
     4741 cgctcggcgg ctcgccagtg catatgttcc tctatgtcga ggacgcggac gcggtgttca
     4801 accgtgccat ctcggcggga gcgaccgagt tgatgccggt cgaggatagc tccgacggcg
     4861 accgtcgcgg cggggtgaca gaccccttcg ggcacgtatg gtacatcggc acccacgtcg
     4921 aggacatctc ccgcgaggaa ctgcagaagc gatacgacga aatgaccagg cagtaacaac
     4981 cgacatagcc gccgggatgc tggcggaggc ccgattcgct agccgcgaag ccctcctccg
     5041 caggcgacat aagccaaacc ctgataccta ttcggtactc gttagaacgc ggcggttgcc
     5101 gtataactag acggtcgtcg atggtccgct ggaggtggga agaatagagt tttcgagaga
     5161 gtcggcgcgg gagctgttgc tcgcggcgca ggggattggc tcctggccgg agcgtcccgc
     5221 gaccaaagag gacgtgctag gagccgtgcg gaagatgggg gtattgcaga tcgactctat
     5281 ctccgtcgtc gctcgcagtc catatctggt gttgtggagc aggctcgggt catacgagcc
     5341 gctatggctg gacgagcttc ttgctgaggg ggcactcttc gagtactggt cgcacgcggc
     5401 ctgcttcatc cccatcgagg actacggtct gtacaggggg ctaatgctcg gtgacaggga
     5461 aaatcccgtg gcctggatgc aggcccaccc cgatgcgata gcgcacgtga tggagtatgt
     5521 tcgcgagaac gggtccgtgc gctcggccga gttccggcgc gccgacgggc gtgccggcgg
     5581 ctggtgggac tgga
//
LOCUS       DATAIR010000762         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_360522, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000762 DATAIR010000000
VERSION     DATAIR010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360522"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VHM69_18130"
     CDS             <1..507
                     /locus_tag="VHM69_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742364.1"
                     /translation="DQKLAEGALAEARRMDAPVARLREELDGGYQIARLSPPRRRHLG
                     NLAYYAVATDQLDLAVRNTRVLSRAALTLVRERGAAPGPLPEAILGLALAVEALAGYL
                     EDPEHPLDVRHFALGAVRDATSVLEYDNDLETSMLVGQIRSTAIDLLQAVGMDYTEAL
                     ESVREGSR"
     gene            complement(587..2395)
                     /locus_tag="VHM69_18135"
     CDS             complement(587..2395)
                     /locus_tag="VHM69_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742365.1"
                     /translation="MKELRAGLRRTTETLSNFTPFLRPRRRGLLLALGVLLLETVTSL
                     SQPWPLALTIDYVLTDNKPLPDVVPGPLADEALLLAGIAFLIVLIFTVTRTVASFRRY
                     LLQRLGQETVFDMREALYSKVHALGLDYHGKRRTGDTITRVTSDVKEVRTMLVDSVVE
                     VFSSVMILLGMLVVMLWLDWQLTLLALVTIPFLFLAVKRYRKALVERMRVVRAREGAI
                     ASVMQEAITGIRAVKLFAREEDEMERFRVESRESLVASVDSAVIEAKFSTVLGIVGGL
                     GTALVVYFGARQVLAGVLTLGDLTVFVAYLALFLSPLWALSRQVNQIGKSLVSGERII
                     DLLNAEPTVKDLPGARPAPALDGRVTFDGVRFAYDVEAGEVLRGVDFDVEAGSRVALV
                     GVSGAGKTTVASLIARLYDPQRGRVLIDGEDVRDFTLKSLRDSITFVPQDPMLFRATV
                     AENIAYGRPGASREEIETIAGLAGADEFIRELPDGYDTLLSERGESLSGGQRQRISIA
                     RAMLRDTPILILDEPQSGLDAEAAAAVEESWKALTEGRTTFVIAHELRLVRSVDLILV
                     IEEGRVAETGNHDDLVSSDGLYARLYSLQERDPVQP"
     gene            2473..>2706
                     /locus_tag="VHM69_18140"
     CDS             2473..>2706
                     /locus_tag="VHM69_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742366.1"
                     /translation="MARRVVAAVEDLLFKSKISETASQLGIEAAFPRNPLKLLEALRE
                     SPPDLLVLDLNSARFEPLSLLRTVKSEERTQNVS"
BASE COUNT          501 a          896 c          823 g          486 t
ORIGIN      
        1 gaccagaagc tggccgaagg ggcactcgca gaggcacggc gcatggacgc tcctgtggcg
       61 cggctgcggg aggagctcga cggtggatac cagatcgcac gcctctctcc gccgcgcaga
      121 cggcatctcg gaaatctggc ctactatgcc gtcgcgaccg accagctcga cctcgccgtg
      181 cgcaacaccc gcgttctctc gcgcgccgcc ctcaccttgg tgcgcgagag aggagcggca
      241 ccgggacccc ttcccgaggc tatcctcggg ctcgccctcg ccgtggaggc gctcgccggt
      301 tacctggagg accctgaaca cccgctcgac gtccgccact tcgcgctcgg tgccgtgagg
      361 gacgctacct ccgtgctcga atatgacaat gatctggaga ccagcatgct cgtcggacag
      421 atccgttcga ccgccatcga tctcttgcaa gccgttggca tggactatac cgaagccctt
      481 gagtcggttc gcgaagggtc acgctaggcg aaggagacgt agatggcgac cgccaccgcg
      541 gtgaagacga tgcacattat gagcatctct ctcaagcgtt cacctcctac ggctgtaccg
      601 gatcgcgttc ctggagcgag tagagcctgg cgtagagtcc gtccgaggac acgaggtcat
      661 cgtggttgcc ggtctcggcg acgcggcctt cttctatcac caggatcaga tctacgctcc
      721 tgaccagcct gagctcgtgg gctattacga aggttgtgcg tccctccgtc aatgctttcc
      781 agctctcctc tacggccgcg gcggcctcgg cgtctagacc cgactggggc tcgtccagga
      841 tcaggatggg cgtgtccctc agcatggcac gcgcgatgga gatgcgctgc ctctgccctc
      901 cactcaggct ctccccgcgc tcggagagca gggtatcgta gccatcgggc agttcccgta
      961 tgaattcgtc agcccccgcg agcccggcta tcgtctcgat ctcctctcgg ctcgcccctg
     1021 gccgcccgta ggctatgttc tcggccaccg tcgccctgaa gagcatcggg tcctgcggaa
     1081 cgaaggttat ggagtcacgc aaggacttga gggtgaagtc cctgacgtct tccccgtcga
     1141 tgaggaccct tcctctctgg ggatcgtaga ggcgggcgat gaggctcgct actgtggtct
     1201 tgcccgcgcc gctcacccca acgagcgcca ccctgctccc cgcctcgacg tcgaagtcca
     1261 cgccccgcag gacctcgcca gcctctacat cgtaggcgaa gcgcacgcca tcgaaggtca
     1321 ccctgccgtc taaggccggc gcgggacggg ctccgggaag gtccttcacc gtgggctccg
     1381 cgttcaggag gtctatgatc cgctcgcccg atactaggga cttgccgatc tggttaacct
     1441 gcctgctcag tgcccacaac ggcgagagga acagggccag gtaggccacg aagacggtca
     1501 ggtcgcccag agttaggacg ccggcaagga cctgcctcgc cccgaagtaa acgacgagcg
     1561 cggtaccgag ccctccgacg atgccgagca cggtgctgaa cttcgcctct atcacggcgc
     1621 tgtccacgct tgcgaccaga gactcgcggc tctcgaccct gaagcgctcc atctcgtcct
     1681 cctcgcgcgc gaagagcttg acggccctga tgcctgttat ggcctcctgc atgacgctcg
     1741 cgatcgcacc ctctctggct ctcacgaccc gcatcctctc gacgagcgcc ttcctgtagc
     1801 gcttgacggc gaggaacagg aaagggatcg taacgagggc gaggagggtg agctgccagt
     1861 cgagccacaa catcactacc agcatgccga gcaggatcat gacggatgag aacacctcga
     1921 ccacggagtc gacgagcatt gtgcgtacct ctttcacgtc gctggtgacg cgggttatgg
     1981 tgtcgccggt acgccttttg ccgtggtagt cgaggccgag ggcgtggacc ttcgagtaca
     2041 gagcctcgcg catgtcgaag acggtctcct gtccgagcct ctggagcaga tagcgccgga
     2101 aggaggcgac ggtcctggtg accgtgaaga tcaaaactat aaggaaggcg ataccggcga
     2161 gcaacaacgc ctcgtccgcc agaggacctg gcaccacgtc aggcagcggc ttgttgtccg
     2221 tgagcacgta gtcgatggtc agcgcgagag gccacggctg cgagaggctc gtcaccgtct
     2281 ctagcagcaa cacaccgagc gccagcagaa gccccctgcg tcggggcctc aagaacggcg
     2341 tgaagttgga aagtgtctct gtggtgcgcc gtagcccggc gcgaagctcc ttcaatcttt
     2401 tcacccggat aggataccgc acagcctgcg ggtagaatgt gcgagcttac ccaaactaat
     2461 cacggagggc acgtggcgag aagagtagtt gctgccgtag aagacctttt gttcaagagc
     2521 aagatctccg agactgcatc ccaactcggc atcgaggcgg cgttcccgcg taaccccctg
     2581 aaattactcg aagccctgcg cgagtcaccg cccgacctgc tcgtcctcga cctgaactcg
     2641 gcccgcttcg agccgctctc gctgctgcgc accgtgaagt ccgaagaaag gacccagaac
     2701 gtgtct
//
LOCUS       DATAIR010000763         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_360798, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000763 DATAIR010000000
VERSION     DATAIR010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360798"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHM69_18145"
     CDS             complement(<1..196)
                     /locus_tag="VHM69_18145"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX2742367.1"
                     /translation="MKKLINAPEGVVEEMLRGLERAHGGLIRLIPEHNVVVRADAPVQ
                     GKVALVSGGGSGHEPTHGGYV"
     gene            complement(250..1770)
                     /gene="glpK"
                     /locus_tag="VHM69_18150"
     CDS             complement(250..1770)
                     /gene="glpK"
                     /locus_tag="VHM69_18150"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX2742368.1"
                     /translation="MAADYILAIDQGTTGTTVLVLNHDSEVVGRAYSEFTQHYPRPGW
                     VEHDANEIWQVSRRVVGTALGDAGISARNLAAVSVTNQRETIVMWDRQTGEPVHNAIV
                     WQDRRTAEFCDELKERGLEGTFRDKTGLVIDAYFSGTKVRWMLDNVEGLRERAQNGEI
                     AFGTIDSWMVWNLTGGQTHITDYSNASRTLMYNIYDLQWDEELLEILEVPKEVLPEVK
                     PSSYVFGETDPASFFEATVPVAGIAGDQQAALFGQACYEPGLAKNTYGTGSFVLMNTG
                     TEAVPSNEGLLTTIAWGIGDEPVQYALEGAIFITGAAVQWLRDGLGIIKDAAETEDLA
                     KSIDSNEGVYFVPALVGLGAPHWDSYARGTIVGLTRGSTKEHLARAALESMAYQTRDV
                     VRAMERDSGIELKDFRADGGAVANTFLMQFQADILGVSVEVPEVTETTAVGSAYMAGL
                     ATGFWESKEDLAERWRLQRRYDPQMGDEERERLHRLWLRAVERAKGWEREEQEAAE"
     gene            complement(2087..2902)
                     /locus_tag="VHM69_18155"
     CDS             complement(2087..2902)
                     /locus_tag="VHM69_18155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2742369.1"
                     /translation="MVQLLRRHPLMTFFVLAFCITWVVWIPRAAGAQLGVLGQLWTWV
                     PAIAALIAAALTGGGAAVRDLGARLVRWRVGWQWYVVTLVGPAVFTLSVAGVYVLLGG
                     SWATAAPPALREGALFLLPLLLLALALTDGLGEELAWRGFALPRLLLRHKALAASLIL
                     GVFWGLWHLPLVWTEGATMYQQPIWLFFVDILAKSVLFTWVFLHTRGSVLLAMLLHAS
                     TNMFIVSPNLTTSGDMTLLLIAAAAKWLLVGAVIVVAGPDLARGPRPEALPAA"
     gene            complement(3073..3654)
                     /locus_tag="VHM69_18160"
     CDS             complement(3073..3654)
                     /locus_tag="VHM69_18160"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEX2742370.1"
                     /translation="MTVDQGAYHFLRALRRNPVIPAVRGPDSALEAALAGDHAAIFVL
                     GGDIFKVLGRVRAQGRRPQVYVNVDLVGGIAGDASGLRFLSGRVEGIISTHRHVIELA
                     KSSGLITIQRLFAIDSGAVERGLKLIRRAEPRFVEILPALAYREIAGRYSEALDLPVL
                     AGGLLKGHEEISTLLKAGAVGVSTSHRGLWNGA"
     gene            3798..4478
                     /locus_tag="VHM69_18165"
     CDS             3798..4478
                     /locus_tag="VHM69_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742371.1"
                     /translation="MPSRVLIADDHALFRAGIRVMLTSRPDIEVVGEAEDGEEAVALC
                     RKTHPDLVMMDLTMPRVDGIAATRTIKSEFPKTSVLVLTAHADEELLMEAVRAGAAGY
                     VIKDAVGPIELLDAVRGVLNGESQVDQDLVMKLVRRLAAEAGTSAKSTAEPTEQPGSL
                     TQRELEVLRLLPLGKTNRQIAAQLHLSLSTVKKRLERIISKLGVSDRTQAAVKAIELG
                     LLGPEQAD"
BASE COUNT          907 a         1590 c         1432 g          848 t
ORIGIN      
        1 cgacgtagcc gccgtgggtc ggctcgtggc ccgagccgcc gcctgagacg agggcgacct
       61 tgccctgcac gggggcgtcg gcgcgtacga cgacgttgtg ctcggggatc agccggatca
      121 ggcctccgtg ggcacgctcc agccccctga gcatctcttc tactacccct tcgggggcgt
      181 tgattagttt tttcatcgcg ctcctttcgg agagatcgag gcttgtgacc ccgatctctc
      241 ctctcaatcc tattccgctg cctcctgctc ctcgcgttcc catcctttgg ccctctccac
      301 ggccctgagc cagagccggt gtagccgctc gcgctcttca tcgcccatct gcgggtcgta
      361 gcgccgctgc agcctccatc tctcggcgag atcttccttg ctctcccaga agccggtggc
      421 cagcccggcc atgtacgccg agcccacggc ggtggtctcg gtgacctcag gaacctccac
      481 cgacaccccc aagatatccg cctggaactg catgaggaag gtattggcta ccgccccgcc
      541 gtcggcccgg aaatccttta gctctatgcc cgagtcgcgt tccatcgccc ggaccacgtc
      601 ccgagtctgg taggccatgc tctccagggc agcccgagcc agatgctcct tggtactgcc
      661 ccgggtgagc ccgacgatcg tacccctggc gtaggagtcc cagtgcggcg cgccaagccc
      721 tacgagagcg ggcacgaagt acacgccttc gttggaatcg atgctcttgg cgagatcctc
      781 cgtctcggca gcgtccttga tgatccctag cccgtccctg agccactgga ccgcggcccc
      841 ggttatgaag atggcgcctt ccagggcgta ctggacaggc tcgtcgccga tgccccaggc
      901 gatcgtggta aggagcccct cgttgctcgg gaccgcctcc gtgccggtgt tcatcagcac
      961 gaacgacccc gttccgtagg tgttcttggc gagacccggc tcgtagcagg cctgcccgaa
     1021 gagcgctgcc tgctggtccc cggctatacc cgccacagga acggtggcct cgaagaagga
     1081 agccgggtct gtctctccga agacgtaaga gctgggcttg acctcaggca gaacttcttt
     1141 gggaacttcg aggatctcca gaagctcctc gtcccactgg agatcgtaga tgttatacat
     1201 cagggtccga gaggcattcg agtagtcggt gatgtgagtc tgaccaccgg tgaggttcca
     1261 gaccatccag gagtctatgg tgccgaaggc tatttcgccg ttctgggccc gctccctcag
     1321 gccctccacg ttgtcgagca tccagcgaac tttggtcccg gagaagtagg cgtctatgac
     1381 cagcccggtt ttgtccctga aggtgccttc gaggccacgc tctttcagct cgtcgcagaa
     1441 ctccgccgtc ctgcgatcct gccagactat ggcgttgtgc accggctctc cggtctgccg
     1501 gtcccacatc acgatagttt cacgctggtt ggtcacgctg accgcagcga ggttgcgcgc
     1561 ggagatcccg gcgtctccca gggccgtacc gacgacccgc cgggaaacct gccagatttc
     1621 gttggcgtcg tgctcgaccc agcccggtct cgggtagtgc tgggtgaact ccgagtacgc
     1681 ccgtcccacg acctcgctgt cgtggttgag gaccagaacg gtcgtccccg tggtcccctg
     1741 gtctatcgcc aaaatgtagt ctgctgccat acccttcctc tctcctttcc caataccaac
     1801 cgtgcaagcc tccactcggg gccgtagatc acctcctcac gacaagaacc ccgcacgaaa
     1861 ccccactttc cggtatcagg gtaccggacc tcgggatctc gtgcgaggct ctctctttat
     1921 ctctgcgcct tcgctacgta tttagtttgg aactcaatgt acttgcaagc cggtcccctg
     1981 tcaaccgtct cggaatggtt agaaagggga ttctgaggat gaggcgcccg gctcgcctgt
     2041 gaggcagttc ggacggtaag cactcgggtg aaaagggcat cacggctcat gccgctggca
     2101 acgcctctgg tcgcggaccg cgtgcaagat ctggccctgc cacgacgatc actgcgccta
     2161 cgagcaacca cttcgccgcc gcggcgatca ggagtagtgt catgtcgccg ctggtggtga
     2221 ggttcggcga cacgatgaac atgttggtcg aggcatgcaa cagcattgcg aggagtacgc
     2281 ttccgcgggt gtgcaggaag acccaggtga agaggaccga cttcgccagt atatccacga
     2341 agaacagcca gatgggttgt tgatacattg tggccccctc cgtccatacc aggggaagat
     2401 gccacagccc ccagaacacg ccaaggatca ggctcgccgc cagggctttg tgccgcaaca
     2461 gtagccgagg aagcgcgaac ccccgccagg caagctcctc gcccaggccg tcggtgaggg
     2521 cgagtgccaa caataatagt ggcagcagaa acaaagcgcc ttcgcgcagg gccggcggcg
     2581 ccgcggttgc ccaggagccg ccaagtagga catagacccc ggctaccgag agcgtgaaaa
     2641 cagcgggccc gacaagggtc accacatacc actgccaacc tacgcgccag cgcaccaggc
     2701 gcgcgcccag gtctctcacc gcggcgcccc caccggtgag ggcggccgcg ataagcgcgg
     2761 ctatagccgg gacccaggtc cacaactgcc ccaggacgcc tagctgggca cccgccgccc
     2821 gcggaatcca aactacccac gtgatacaga acgcgagcac gaagaaagtc atcaaggggt
     2881 ggcgccggag caactgaacc atatccctac ctcctgcctt tccacgccat gggtacacca
     2941 tactcccggg aaagtggaag gtacatagga catgtgtttg taagtggtat tgaccgggcg
     3001 ctctactcaa tcagggcgag tatccgatga cacgccttct gaaaatcgcg cgaaccccta
     3061 tcagaaggct tctcaggcgc cgttccacag ccccctgtgg ctcgtcgaga cgccgaccgc
     3121 gccagccttc aggagggtgg atatctcctc gtggcccttg agcaaaccgc ccgcgaggac
     3181 ggggagatcc agggcctcgg agtagcgccc ggctatctcg cggtaggcga gcgccggcag
     3241 tatctccacg aagcggggct ctgcgcggcg gatgagcttc aggccccgct cgacggcccc
     3301 tgaatcaatg gcgaagaggc gttggatcgt tatgaggccg gaggacttgg cgagttctat
     3361 aacgtgtctg tgcgttgaga tgatcccctc caccctcccc gagagaaaac gcagcccgct
     3421 tgcgtcgccc gcgataccgc cgacgaggtc gacgttcacg tagacctggg gcctgcggcc
     3481 ctgtgcgcgg accctcccaa gcaccttgaa gatgtcgccg ccgagcacga agatcgccgc
     3541 gtgatccccg gcgagcgcgg cttccagcgc gctgtcaggc ccgcgaacag ccgggatcac
     3601 ggggttccgc cgcagcgccc ttaggaagtg atacgcccct tggtcaaccg tcatccgcac
     3661 accatgactc taagagatat tcgcgccgcg ctcaacccgg cacgcactcg tcggcgggaa
     3721 accgctcatt tacggtatca ttcagccgag catgactgca ggatgcggcc atcggggagc
     3781 aagcgcagga gagagaattg ccatcacgag ttttgatcgc agacgaccac gcgctgttca
     3841 gggctgggat cagggtaatg ctgacttcgc ggcccgatat cgaggtggtc ggcgaggcgg
     3901 aagacgggga ggaagccgtc gcgctctgcc gcaagacgca ccctgacctg gtgatgatgg
     3961 atctcacgat gccgagagtc gacggcatcg cggccacccg cacgataaag tccgagttcc
     4021 cgaagacgag cgtcctcgtg ctgacggccc acgcagacga ggagcttctg atggaggccg
     4081 tgagggcggg ggctgcagga tacgtcatca aggacgccgt cggaccgatc gagttgctag
     4141 acgccgtccg tggggtccta aacggtgagt cacaggtgga tcaggacctc gtcatgaaac
     4201 tcgtccggcg cctggccgcc gaagccggga cgagcgcgaa gtcgaccgcc gaaccaactg
     4261 agcagccagg gtctctcacc caacgcgagc tcgaagtcct tcgactcctg ccccttggga
     4321 agacgaaccg ccagatcgcc gcccagctgc acctctccct ctccacggtc aagaagcgcc
     4381 tggagcgcat catctccaag ctcggcgtct cggaccgtac ccaggccgcc gtcaaggcca
     4441 tagaactcgg cctcctgggt cccgagcaag ccgactagac gccatccggt agcacctttc
     4501 ccgaaaagca gccacatgac cccctgcaaa atgggccaca tgaccccctg gaaatggcca
     4561 catgactggt gcgtcaatcc ccggcgctcc tccatacttc gcgttatcgc ggcgcgaagc
     4621 agcgctttgg ccgaaggggc agagccctcg actcattcgc gacggatggg cggggtgatg
     4681 gtctttggcc gacggggcag ggtcctcgcc cctatttcaa aagagcgagg agcagagcca
     4741 atgcctaaaa gagaacaccc gcacgagcat ccgccag
//
LOCUS       DATAIR010000764         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_223137, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000764 DATAIR010000000
VERSION     DATAIR010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223137"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..983
                     /locus_tag="VHM69_18170"
     CDS             69..983
                     /locus_tag="VHM69_18170"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2742372.1"
                     /translation="MRVGGQAIMEGVLMRSPNFWGIAVRTPSGDMDLRAERFRSITRK
                     NKLFRLPIIRGALSLGETLWLGMKALTLSTNIALGEEQELSKKEIAGTLIFALVLGFG
                     LFLVAPVMGTKGLGSLIGSSIENPILFNLIEGVIRIAIFIAYLLGITLISKDIKRFFA
                     YHGAEHKAIKVYERGEELVPENARKLDTSHVRCGTSFILYVLVLSILVFSLLGVEGWL
                     YMLASRVVVIPLVAGIAFEFIMWSARNQDNAIVRALVWPGLQMQKLTTREPSDDMVEV
                     AMASLKKVLSMEREAEIEPNEATNKLVI"
     gene            985..2079
                     /gene="prfA"
                     /locus_tag="VHM69_18175"
     CDS             985..2079
                     /gene="prfA"
                     /locus_tag="VHM69_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX2742373.1"
                     /translation="MDSQVVKLAEETLGRYDALTQELSDPNIFNDQRHYAEVAKEHAR
                     MRRGAEMSRELLDAVEAERDARELIPQAENTEEREFFTAEARDAEERIDDLSERIRTE
                     LIDRDPNDDKDVIIEIRAGAGGDEAALFAGELHDMYTRYADRLGFKHSTLSSSPAEVG
                     GYKEVAVEISGDRAFSIFKHEGGTHRVQRVPKTESQGRIHTSTATVAVLPEAEDVEVQ
                     INAGDLEVDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVTCQNEKSQLQNKEQAMKIL
                     KSRLLEREMRERAEQEGALRQAQVGTGDRSAKVRTYNFPQGRITDHRVGVTSHNLEAT
                     LGGDLEEFTQALAAKERSDKLAAEAAGTPG"
BASE COUNT          448 a          682 c          706 g          330 t
ORIGIN      
        1 ggtggtaggg tccagcgctt ccagaaccgc cgccaggctc gccagaacag gcagtaaggc
       61 cgaacgcggt gagggtcggc ggacaggcca taatggaggg cgtactgatg cgctccccca
      121 acttctgggg gattgccgtc cgcacgccct cgggggacat ggatttgcgg gcggagcggt
      181 tccgttcgat cacccgcaag aacaaactct tccggcttcc cataatccgg ggggccctct
      241 ccctgggaga gaccctctgg ctcggcatga aggcgcttac cctctcgacg aacatcgcgc
      301 tcggcgagga gcaggagctc tccaagaagg agatagcggg taccctcata ttcgcgctcg
      361 tgctcggctt cgggctcttc ctcgtcgcgc cggtaatggg gaccaagggg ctcggatcgc
      421 tgatcggttc gagcatcgag aaccccatac tcttcaacct cattgagggt gtgatccgga
      481 tcgcgatctt catcgcttac ttgctcggaa tcaccttgat cagcaaagac atcaagcgct
      541 ttttcgccta ccacggtgcg gagcacaagg cgataaaggt ctacgagcgc ggcgaggaac
      601 tggtccccga gaacgcccgc aaactggaca cgagccacgt ccgctgcggg acgagcttca
      661 tcctctacgt gctcgtcctc tcgatcctgg tgttctcgct cctcggggtc gaaggctggc
      721 tgtacatgct ggcgagccgg gtggtcgtca tcccgctcgt ggcagggata gccttcgagt
      781 tcatcatgtg gagcgcgcgc aaccaggaca acgccatcgt tcgggcgctc gtctggcccg
      841 gactacagat gcagaagctg accacgcggg agccctcgga cgacatggtc gaggtcgcga
      901 tggcctccct gaagaaggtc ctctcgatgg agagggaagc agagatcgag cccaacgagg
      961 ctacgaacaa actggtgatt tgaattggat agtcaggtag ttaagctggc cgaggagacg
     1021 ctcggccgct acgacgcgtt aactcaagag ctttcggacc cgaacatctt caacgaccag
     1081 cggcactacg ccgaggtcgc caaggagcac gcgcgtatgc ggcgcggggc cgagatgtcg
     1141 cgggagttgc tcgacgcagt agaggcggag cgcgacgcgc gcgagctgat cccccaggca
     1201 gagaacacgg aggagcgcga gttcttcacg gccgaagcga gggacgcgga agagcggatc
     1261 gacgatctct ccgagcgcat caggaccgag ctcatcgacc gcgacccgaa cgacgataag
     1321 gacgtcatca tcgaaatccg ggccggagca ggcggcgacg aggcggcgct cttcgcgggc
     1381 gaattgcacg acatgtacac ccgctacgcc gaccgtctcg ggttcaagca cagtaccctc
     1441 tcctctagcc ccgcagaggt cggaggctac aaggaggtcg ccgtggagat cagcggcgac
     1501 agggcattct cgatcttcaa gcacgagggg gggacgcatc gcgtccagcg ggtgccgaag
     1561 acggagagcc agggacgcat tcacacctcg accgcgacgg tggccgttct tcccgaggcc
     1621 gaggatgtcg aggtccagat caatgccgga gacctcgagg tggacgtgta ccgttccagc
     1681 ggacccggcg gccagagcgt gaacaccact gattccgcgg tcaggatcac acacaagcct
     1741 accggcctgg tcgtcacctg ccagaacgag aagagccagc tccagaacaa ggaacaggcc
     1801 atgaagatcc tgaagtcccg cctcctggaa cgagagatgc gcgagcgcgc cgagcaggaa
     1861 ggcgcactcc gccaggcgca ggtaggcacc ggagaccgtt cggcgaaggt ccgcacctac
     1921 aacttcccgc agggacgcat caccgaccat cgcgtcggtg tcacctccca caacctcgaa
     1981 gctaccctcg gcggcgacct ggaggagttc acgcaggcgc tcgccgccaa ggagaggtcg
     2041 gacaagctcg ccgccgaagc cgccgggacg ccaggctaaa acgtgcgtgc cggcgcgacg
     2101 agcgcccgag aggccgcgcg gctcgcggcg gcaaggctga agcgtgccgg catccccgag
     2161 cccgtc
//
LOCUS       DATAIR010000765         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_223421, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000765 DATAIR010000000
VERSION     DATAIR010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223421"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..756)
                     /gene="rpe"
                     /locus_tag="VHM69_18180"
     CDS             complement(91..756)
                     /gene="rpe"
                     /locus_tag="VHM69_18180"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2742374.1"
                     /translation="MTFFGKLGGRVIGAPSILAADFAHLAEEVRRAERGGAELVHFDV
                     MDNHFVPNLTVGPAVAASLAAAVDLPIDAHLQVTNPDNLIPAFIEAGVVSVSVQPEVV
                     IHLHRTLEMIRAGGCAAGVALNPTTPPELIEWALPYVSYVNVMSVNPGFGGQAFIPEM
                     VEKVRRLKEMTDVPIEVDGGITVQTAPLMVEAGAQVLVAGSAVFKGDPEAEMRRIIEA
                     GRK"
     gene            complement(848..>2376)
                     /locus_tag="VHM69_18185"
     CDS             complement(848..>2376)
                     /locus_tag="VHM69_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742375.1"
                     /translation="GLSRAKINGWLLEQAAASRVSGIDPVHFVFSYLNPTLVVVAFLV
                     FFALARILFKSERAALFSGCLYALFFAIHLSQSRIALGGEFIQRLPEDKLAAKFLFLP
                     LALAFAVAFMECGRKRYFCGFAFLCCAVMAIHPIGLAITGISMAGFAILHLASNPRSR
                     VAWARISAMGLAGLLVVAVPAVLVSFFTDEPLANALADSDINSGDPDVLRNMIFVSPE
                     RSRIFEFANGSYMMHPSLLLDEVIAVAFLIGLPFLLWRLRRSLAAQLLFGMMYLTSVV
                     VYVPPIATFLGDEVVLPGQIWRLAWPIQLAALLTLGWVVWTAMSYVMAWLEGYRPLRF
                     LAGAIPVLVVVVLTVAAVPLTRPGIESIQTHREASVSSGYYPADPIYPWFRDELRSTV
                     VVMAPDNQSARIPAYSSEANVVSRRGGLVLNVLGKLEERVPGQIEVPQGSLDVRAFFR
                     GTTLEKGMEILRRNQVDYVMVSQESQLDASLDRLPGFEPVDEPSDRYDLYSVDLGKIP
                     "
BASE COUNT          493 a          734 c          765 g          384 t
ORIGIN      
        1 cccgcgacga tagccaggtc gggccgatac ccacatctcg acgccgagct accccaataa
       61 ctctgatgcc tgtagcctga tgcctgatgc ctatttccgg ccagcctcga tgatcctacg
      121 catctcggcc tcggggtcgc ctttgaagac cgctgacccg gcgaccagga cctgggctcc
      181 ggcctcgacc atcagcggcg cggtctggac ggtgatacca ccgtcgacct ctatcgggac
      241 gtcggtcatc tccttgaggc gcctgacttt ttcgaccatc tcggggatga atgcctggcc
      301 tccgaagcca gggttgacgc tcatgacgtt cacgtagctc acgtaaggca gggcccactc
      361 tattagctcg ggaggggtcg tcgggttgag ggctacgccg gccgcgcagc cgccggcccg
      421 gatcatttca agcgtgcggt gcaggtggat gacaacctcg ggctggacgg agacgctgac
      481 cacgcccgct tcgatgaaag cggggatcag gttgtccggg ttggtcacct gcagatgggc
      541 gtcgatgggc agatctaccg cggcggcgag cgaggccgcc acagccggac cgacggtcag
      601 gttcgggacg aagtggttgt ccatgacgtc gaagtggacg agctcggcac cacccctctc
      661 ggccctgcgc acctcctcgg ccagatgggc gaaatccgcc gcgagtatcg aaggggcacc
      721 gatcaccctg ccaccgagct ttccgaaaaa cgtcaagcgc actgcctcct aagcgaaagg
      781 tccaaaaaca accattttac agaaaggacc gaaggaggcg ttggatctcc tcatgaatac
      841 gggtcctcta gggaatcttg ccgaggtcca cactgtacag atcgtacctg tcgctcggtt
      901 cgtccacggg ctcgaagccg ggcaggcggt cgagtgatgc gtcgagttgc gactcctgtg
      961 agaccatcac gtagtccacc tggttgcggc gcaggatctc catccccttc tccaaagtgg
     1021 tccccctgaa gaacgcccgc acgtcgagcg agccctgggg gacctcgatc tggcccggca
     1081 cacgctcctc tagcttgccg aggacgttga ggaccaaccc gcccctgcgg ctgacgacgt
     1141 tcgcctccga ggagtatgcg gggatgcggg cactctggtt gtccggggcc atgacgacga
     1201 cggtcgagcg cagctcatca cggaaccatg ggtagatggg atccgcgggg tagtagccgg
     1261 acgaaacgct cgcctccctg tgagtctgta tcgactcgat tcccggacga gtgaggggca
     1321 cggccgcgac cgtcaggacg accacgacca ggaccgggat tgctccggcg aggaagcgca
     1381 agggccggta cccctcgagc cacgccatca cgtaactcat cgcggtccat actacccagc
     1441 cgagcgtgag cagcgcggcg agctgtatgg gccaggccag acgccatatc tggcctggaa
     1501 ggaccacctc gtccccgagg aacgtggcta tcgggggcac gtacacgacg accgaagtca
     1561 ggtacatcat tccgaacagc aactgggccg cgaggctgcg tcttagccgc cagagcaaga
     1621 acggaagccc gatcaggaac gcgacagcga tcacttcgtc gagtagtagc gaaggatgca
     1681 tcatgtaaga gccgttcgcg aactcgaaga tgcggctcct ctccgggctc acgaagatca
     1741 tgttcctcaa cacgtcgggg tcgccagagt tgatgtccga gtcggccagg gcgttcgcca
     1801 gcggctcatc tgtgaaaaag gagacgagga cggccggaac ggcgacgacg aggagcccgg
     1861 cgagacccat ggccgagatc ctggcccaag ccacacgact tcgggggttg gacgccaggt
     1921 gcagtatcgc gaagcccgcc atggagatgc ccgtgatcgc cagtcctata gggtggatcg
     1981 ccatgacggc gcagcacagg aacgcgaagc cgcagaagta tcgttttctt ccacattcca
     2041 tgaaggcgac ggcgaaggcg agggcaaggg gcaagaagag gaacttcgcc gcgagcttgt
     2101 cctcgggaag gcgctggatg aactcgcctc ccagcgcaat gcgcgactgg ctgaggtgga
     2161 tggcgaaaaa gagcgcgtag aggcacccgg agaagagcgc cgccctctca ctcttgaaca
     2221 gtatgcgcgc cagggcgaag aaaacgagga aggccaccac aaccagtgtg gggttcaggt
     2281 acgagaaaac aaagtgcacg gggtcgatcc cggagaccct cgaggcggcc gcctgttcca
     2341 gcagccagcc gttgattttg gcgcgggaga ggccca
//
LOCUS       DATAIR010000766         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_361094, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000766 DATAIR010000000
VERSION     DATAIR010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361094"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="ribA"
                     /locus_tag="VHM69_18190"
     CDS             complement(<1..465)
                     /gene="ribA"
                     /locus_tag="VHM69_18190"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HEX2742376.1"
                     /translation="MPSIRRVARAGLPTSHGGFEMFVYEAADGKEHVALTSGFEPGSV
                     GGPILVRLHSECLTGDVFGSRRCDCGEQLEESMRLLREQGRGVLLYLRQEGRGVGLAN
                     KISAYALQEQGLDTVQANQALGLPDDARDYRVAAEMLLDLGVRRAAVLTNNPA"
     gene            578..964
                     /gene="folB"
                     /locus_tag="VHM69_18195"
     CDS             578..964
                     /gene="folB"
                     /locus_tag="VHM69_18195"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2742377.1"
                     /translation="MSEDRILLSEMVFHGRHGTLPAEHELGQPFVVSVELQLDLRPAG
                     TSDDLTKTVDYSEVHRMAREIVEGRPVQLVETLAERIAAAALDDHPTVEAVKVRVAKP
                     HVRLGETVLAGSAVEVLRRREPGTRS"
     gene            1116..>1876
                     /locus_tag="VHM69_18200"
     CDS             1116..>1876
                     /locus_tag="VHM69_18200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2742378.1"
                     /translation="MDSASRLSTTVSAGVSSPESEARLEHLTQLCVDDLISAFGLGGM
                     WHGRAVLESISRIPARRLARQVLTYDRIVGESGLGTGGAWALKRLSRHAEIEGQVNVP
                     REGPLLLVSNHPGLADAVALFASTPRDDLRVIAADRPFLDALPNTSRYLLTVAEGSAG
                     GSGVARAAARHLRRGGAVLTFPGGRIEPDPAVLPGAVEALDLWSSSADLFARLTPGLA
                     VVPVVVSGVISPSALRIPLTRLRRRRRDREWLAASL"
BASE COUNT          299 a          636 c          624 g          317 t
ORIGIN      
        1 ggccgggttg ttagtgagca cggcggctct tcgcacgccg aggtcaagga gcatctcggc
       61 ggcgacgcgg tagtcgcgcg cgtcatcggg cagcccgaga gcctggttcg cctggacggt
      121 gtcgagcccc tgctcctgga gagcgtaggc ggagatctta ttggcgagcc cgacgccgcg
      181 cccctcctgc ctcaggtaga gtaggactcc gcgcccctgc tcgcgcagta gcctcatgct
      241 ctcttcgagc tgctcgccgc aatcgcagcg gcgcgagccg aagacgtctc ctgtcaggca
      301 ctccgagtgc aggcgtacca ggatcggccc gccaacgcta cccggctcga aaccgctcgt
      361 gagcgcaacg tgctccttac cgtccgccgc ctcgtagacg aacatctcga agccgccgtg
      421 ggaggtcggc agccctgctc gtgcaacgcg tcggatcgaa ggcatggttg tccgatctag
      481 gtcgtggtcg agtgcccgaa attctactac agccgacttt tggtactaac ttacgttctg
      541 gcatactgca tcggatggac cggggcgaaa aataacggtg agcgaggacc gcatactcct
      601 ttcggagatg gtcttccacg gccgtcacgg aaccctgcca gccgagcacg agcttggcca
      661 gcccttcgtc gtgagcgtcg agttgcaact cgaccttcgg cccgcaggga cctcggacga
      721 cttgacgaag accgtagact acagcgaagt gcaccgcatg gcgcgcgaga tcgtcgaggg
      781 acggcccgtg caactcgtcg agaccctggc ggagaggatc gccgcagcgg cgctggacga
      841 ccatcccacc gtagaggccg taaaggtgag ggtggcaaaa cctcacgtcc ggctcggcga
      901 aacggtactg gccggttccg cggtcgaggt cctgcgccgt cgcgagcccg gtacgcgtag
      961 ctaggggttc cggcgggcga accaccgccg tgctccgtgc gtactaacaa ttgacgtgga
     1021 cgccgtcgag gcaggcgaga agggtggcac gctggtgacg aacgggctca ctgataccgc
     1081 cgtacccggc gttgatgcgg ccagggagga tcactttgga tagtgcctcc aggttgtcca
     1141 cgactgtctc ggccggtgtt tctagtccgg agagcgaggc gcgtctcgaa catctcacgc
     1201 aactatgtgt ggacgacctc atctcggcct tcggcctggg cgggatgtgg cacgggcgcg
     1261 cggtactgga gtctatctcc cggatacccg cccggcgcct cgcgcgccag gtcctgacct
     1321 acgacagaat agtcggcgaa tcgggcctcg ggacgggcgg cgcgtgggcg ctgaagaggc
     1381 tctcccgcca tgccgagatc gaagggcagg tgaacgtgcc gcgcgagggg ccgctgctcc
     1441 tcgtctcaaa ccatcccggc ctcgcggacg ccgtcgccct gttcgcctcc acacctcgcg
     1501 acgacctgcg cgtcatcgcc gctgaccgtc ccttcctcga tgccctcccg aacacctcgc
     1561 ggtacctcct gaccgtagca gaggggtcgg cgggaggctc gggggtcgcg agggcggcgg
     1621 ccaggcatct gcggcgaggc ggagcggtac tcacgttccc aggcggcagg atagagcctg
     1681 acccggcggt actgcccggc gctgtcgagg cgctggatct ctggtcgtcc agcgccgacc
     1741 tcttcgcgcg cctcacgccg ggactcgccg tcgtccccgt cgtggtcagc ggtgtgatct
     1801 caccttccgc cctgcgcata cctctgaccc gcctgcgccg ccgccgccgc gaccgcgagt
     1861 ggctcgccgc ctcact
//
LOCUS       DATAIR010000767         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_223623, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000767 DATAIR010000000
VERSION     DATAIR010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223623"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..917
                     /gene="ispE"
                     /locus_tag="VHM69_18205"
     CDS             51..917
                     /gene="ispE"
                     /locus_tag="VHM69_18205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX2742379.1"
                     /translation="MREVRLRAYAKVNYALDVLRLRGDGYHEVRTVMQSISLADEVEL
                     RRSTGGFELSTVPEEVGPLEHNTVYLTWKSLRTLTGDELPVSVTLRKKIPAGAGLGGG
                     SADAAAVLVGLNELFGLGLRVEDLREAGAEIGADVPFCISGGTALGEGVGEVLTRLPA
                     PPEHRLIVAKPRMSADTGIVYCRFDEAKTESIRAVDPVISALRSGNLSALAASVGNDL
                     RYVTAGFVPEVTELEQMLLASGALGASMSGSGTAVYGIFGDEEAAGIAKDRIETPFVG
                     DYEPVSRGVEIL"
     gene            978..1352
                     /locus_tag="VHM69_18210"
     CDS             978..1352
                     /locus_tag="VHM69_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2742380.1"
                     /translation="MGEACVVLLENERREWELPGGKLEVGESPEECLVREIRGELSLD
                     VEVGPLIDAFVYEVLPGVHVLVLTYGCFAEDLRRISHSAEHSAVGLFGLDELEGIDLP
                     RGYERSVRTWSRRTGLQRQPGF"
     gene            1392..1462
                     /locus_tag="VHM69_18215"
     tRNA            1392..1462
                     /locus_tag="VHM69_18215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1424..1426,aa:Gln,seq:ttg)
     gene            1508..>2044
                     /locus_tag="VHM69_18220"
     CDS             1508..>2044
                     /locus_tag="VHM69_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX2742381.1"
                     /translation="MSQSSPIFAVILAAGKGTRMKSNRAKVLHTLCGVPMVNYVIEAT
                     RPLLPEGLFVVVGHQAELVRAVLPEDAEPVLQKEQRGTGDAVRFALDALEGEEEGVLL
                     VINGDGPLISDRTLGELVERHRSAGVGATVLVAELDDPSGLGRVVEDAGVVRIVEERE
                     ATEMEREIRLVNLGLYAFE"
BASE COUNT          363 a          557 c          738 g          386 t
ORIGIN      
        1 gcgcctgaag acttcgtcgg gttgtacgcg gcgctttcct gggcgctgtg atgagggaag
       61 tccggctcag ggcgtacgcg aaggtcaact acgcgctcga cgttctcagg ctgcgcgggg
      121 acggttacca cgaagtccgt accgtcatgc agagcatctc gctcgccgac gaggtggagt
      181 tgcggcgctc cacgggaggc ttcgagctct ccaccgtgcc tgaggaggtc gggccgctag
      241 agcacaatac cgtctacctc acctggaagt cgctgcgaac gcttaccggc gacgagcttc
      301 cggtcagcgt gacgttgcga aagaagatcc ccgcaggggc tggtctaggc ggcgggtcgg
      361 ctgatgcggc cgcggtcctc gtcgggctga acgagctctt cggcctcggt ttgcgggtcg
      421 aggacttgcg ggaggccggg gctgagatcg gggccgacgt gccgttctgt atctcggggg
      481 ggacggcgct cggtgaaggc gtcggggagg tccttacgcg gcttcccgcg ccgccggagc
      541 accgcttgat cgtagccaag cctcgaatga gcgcggatac gggcatcgtc tactgccgct
      601 ttgacgaggc gaaaacagag agcatacgag ctgtcgatcc ggtcatctca gccttgcgtt
      661 cgggaaacct ctcagcactc gccgcgtcgg ttggcaacga cctgcggtat gtaaccgcgg
      721 gtttcgtccc tgaggtgacg gagctagagc agatgcttct agcatccggg gcgctcgggg
      781 cttcgatgag cgggagcggg acggccgtct acggtatttt cggagatgag gaggcagccg
      841 ggatcgctaa agacaggatc gagacaccct tcgtcggcga ctacgagccg gtttctcgcg
      901 gggtggagat cctctgaatg gacgatcaga cgcacggaag tggaatcctg tttcccgtct
      961 ctgtcaaagg tgtattgttg ggtgaagcct gcgtcgtttt gctcgagaac gagcggaggg
     1021 agtgggagtt gcctggcggt aagctggagg taggggagag tccggaggag tgccttgtca
     1081 gggagatccg gggggagctg agcctcgatg tggaggtagg tcccctaatt gacgcgttcg
     1141 tgtacgaggt gcttccggga gtccacgtgc tcgttttgac ctatggttgc ttcgcggagg
     1201 acctgcgtag gatctcgcac tccgcggagc actcggccgt gggtctattc ggcctcgacg
     1261 agcttgaagg catcgatctg ccgcggggat acgaacgctc tgtccgaacg tggtcccgcc
     1321 gcaccggatt acaaagacaa cccgggttct gacggcgaaa aggtgctccg gtataatcgc
     1381 ggcgccggta ctggggcgtg gccaagtggc aaggcaccgg gttttggtcc cgggatcgca
     1441 ggttcgaatc ctgccgcccc agccgaggag cacgcgggta tactaccgat aggagttgcg
     1501 cgagtacatg tcacagagtt cgccgatctt cgccgtgatc ctggcggccg gcaaaggtac
     1561 ccggatgaag tcgaaccggg cgaaggttct gcacacccta tgcggtgtac cgatggtcaa
     1621 ctacgtgatt gaggccacaa ggccactcct ccctgagggg ctcttcgtcg tcgttgggca
     1681 tcaggcggaa ctggttagag ccgtactgcc agaggacgcg gagccggtct tgcagaaaga
     1741 gcagcgtggc acgggagacg cggtgcgctt tgcccttgac gcactcgaag gggaagagga
     1801 aggcgtcctc ctggtgatca acggtgacgg gcccttgatc tcggacagga ccctcgggga
     1861 actggtcgag aggcaccgct cggcgggcgt tggggcgacg gtactcgtgg cagagcttga
     1921 cgacccgagt gggctaggac gggtcgtcga ggacgccggc gtggtcagga tcgtcgagga
     1981 gagagaagcg acggagatgg agcgcgagat caggctcgtc aacctcggcc tctacgcttt
     2041 cgag
//
LOCUS       DATAIR010000768         8958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_268447, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000768 DATAIR010000000
VERSION     DATAIR010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268447"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VHM69_18225"
     CDS             complement(<1..759)
                     /locus_tag="VHM69_18225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742382.1"
                     /translation="MRRLLALSCAIVLVDTIFYAALTPLVPYFNQEFGLSKSAVGLLS
                     GAYGAGVLVGSAPGGYLASRSGVRITALVGLILLSITSVMFALVDTSGLLVAARFVGG
                     FGSALSWVAAFTWLVGRAPEERRAEMIGVMISAAVVGAILGPVLGSVAATVGLLPAFA
                     SVAVAGGVIAIWAALEPAPATSGYVSLMPTLRAVWQPRLLTSLWFIGLSPLLFSGLAV
                     LVPLELYSLGWGAAAVGTVFLISAAFEALVHPLLG"
     gene            834..1181
                     /locus_tag="VHM69_18230"
     CDS             834..1181
                     /locus_tag="VHM69_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742383.1"
                     /translation="MFVVKTISTIITGWLAWERRSQGNKTLFVLSLSLAAFVTLAFAW
                     ASLNPDPGNLARYFAFYWVWGLCALGVLLGMAEYKTSPLRKRRERGHLFILLYIGALL
                     IVTRALNMVLFYG"
     gene            1186..1833
                     /locus_tag="VHM69_18235"
     CDS             1186..1833
                     /locus_tag="VHM69_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2742384.1"
                     /translation="MYRALLFDLDGTLAETDSLHLPTWVDALEPYGVDVDEEFYRERI
                     SGRSTGEIVRDLLPDLTDDEGESIGDAKEASFRERAAELEPLPGLVDFIDQGRERGMK
                     IALVTNAPKENVEAILRSLRLQESFDVVVLADDVEAVKPDPAPYRAALKKIDVPAEEA
                     MAFEDSISGISSSVAAGIPTVGIASTQDPEKLIGAGAFMTAKDFTDPDLRSMIGA"
     gene            complement(1796..2722)
                     /locus_tag="VHM69_18240"
     CDS             complement(1796..2722)
                     /locus_tag="VHM69_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2742385.1"
                     /translation="MRRTLRRALVLLVTLSLGYIGVGLLVVLLMTSPRRKRPEATPAS
                     VGLGYREVEIQSTDGVNLSAWWVPAEDSSRAAVLVPGWGGYKFDEKLLRTVPVYHNAG
                     YGVLLLDLRAQGESSGARRTLGYREMRDVRGALAWLDQQGYKAEDVVLHGWSMGGSTA
                     LRAAPGTGVAAVVEEAGYGDLPLLLKVKIPEFIPFGRMLRPAILLAGSLFPDFDPWEV
                     VPKVDAAKLSDEGVPLLIIHSTADDIVPYEQARILVEAGPEASLWKLEGYGHVEAYEH
                     PEYALKLRAFLERSSRGQTAPMRRSWISDQGR"
     gene            complement(2748..5174)
                     /locus_tag="VHM69_18245"
     CDS             complement(2748..5174)
                     /locus_tag="VHM69_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEX2742386.1"
                     /translation="MSETYEKQPETGGNYEPLGTGELLDHDDRGLRVRAGSTTVEVTA
                     LAPDLFRVGMFPAGRPPRYDSEAIAKEDWETVEVSMQESDEELTLSTTAATARVSLRP
                     LRVGFSDHSGRQFAVDDEELGMGAVETPGADIFSEPLGSPVRLYKKREEGERYFGCGE
                     RTSGLEKTGSHQVFYNVDPPLGHTASFNNLYSSIPFTFSMTNGKAHGLFFDNTHRVEF
                     DLALEDEDRAYYGAEGGAIVYYVFCGPTPRDVVDRYTELTGRTPMPPMWSLGNQQCRY
                     TYMNEEEVREVARGFRERDIPCDVIYLDIHYMDGFRVFTWNKERFPDPQRLISDLREQ
                     GFRVVTIVDPGVKVDENYSVYTDGREKDLYCKTHDGEEYHNAVWPGVCAFPDFTNPDT
                     RRWWGENQSALTDEGVAGIWCDMNEPALFIPKATMPDDVVHPGGRTGEAKWHAQIHNT
                     YGSLMARAAREGLLALRPDERPFVITRAGYAGLQRHAMQWTGDNSSWWEHLWMSMPQL
                     QNLALSGIAWAGVDIGGFGGDTNAELLARWTEFGVFQPFCRNHTMLGTRHQEPWAFGE
                     PCESVCRKMIKLRQRLLPYFYSLFDECHRTGAPILRPLLFEYPDDETTYSADDEFLLG
                     DALLIAPISRPGIEHRHVYLPEGTWFHYWSGERFEGPAHVLAHAPLGEPAIYVKANTP
                     APMGPDAAHTGERPTDPLTLLVFPAEGAGESTIYEDAGDGFGYEAGEYARRTISCESS
                     DNRITIRLGEREGSFAPERGEVRLELRGVTAAQGVTVNGEAREPDLGENVLTVSLGEE
                     EGPVTVEVIL"
     gene            complement(5259..6428)
                     /locus_tag="VHM69_18250"
     CDS             complement(5259..6428)
                     /locus_tag="VHM69_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742387.1"
                     /translation="MFANGPQLALSVPRFAVLGVFFVNGVVIGTWVVRIPAIQQELDL
                     SEGQLGLALLGAAVGALVAMPLVGALVSRFGSRRVVGTTAMLLSLSLVLPALAPSMIL
                     LVPALAMIGAANGGLDVSMNAQAVAVEEGYGRPIMNSFHAAWSFGGLAGAAVGGLFAS
                     LGMGPLPHFASIAVLSAIAFAAVYKKLLPAGADASEDGAPAFARPTRALMGLGIISFC
                     VLLGEGAMGDWSAVYLNNTLQTGPGFAAAGYAAFSLAMAFGRLFGDRFTERLGPTRLV
                     RLCGVLSAIGLGVALAVGQPLVALVGFACAGAGFSIVFPVALSAAGRTKGMAAGPALA
                     AVSTTAYTGFLVGPPFIGFVAEWTSLGAALYLVVALSTVVILLAGAVSAGGGKAK"
     gene            complement(6428..7378)
                     /locus_tag="VHM69_18255"
     CDS             complement(6428..7378)
                     /locus_tag="VHM69_18255"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX2742388.1"
                     /translation="MTLFLTENEVESLLDMPSTLDAVEAVLRQHAEGRATNRARRRVA
                     LPTGGLNYMAAGAPEIGALGLKAYTVARTGARFYTMLFDPEGGELLSIMQSDRLGQQR
                     TGAASGVATKHLAREDANTLGIYGAGWQAESQLEAIASVRDLDRVIVYSRREDSRKAF
                     AEKMGKRLGMEIETTHAAEEPAAQDIVVTVTSSREPVLLGEWLRPGAHVNAAGSNFLF
                     KSEIDREVVRRASFVCADSREELGLEAGDLMLSLETGAIFPEAVYELGQVIAGQVPGR
                     RGPEDITLFASQGLALEDMAAARVVYDRAVERDVGRDIDF"
     gene            7430..7993
                     /locus_tag="VHM69_18260"
     CDS             7430..7993
                     /locus_tag="VHM69_18260"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX2742389.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVLVRRNDEVIPEEVSELH
                     PDRIVVSPGPCTPNEAGVSVELIEKVSNEVPILGVCLGHQSIGQAFGAKVVRGEPVHG
                     KTARIMHDGEGVYRGLDQGFVATRYHSLVIDPESVPDCLVVTSRTEDGTIMGVRHREY
                     PVEGVQFHPESVLTRSGHDLLKNFLGL"
     gene            8003..>8958
                     /gene="trpD"
                     /locus_tag="VHM69_18265"
     CDS             8003..>8958
                     /gene="trpD"
                     /locus_tag="VHM69_18265"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX2742390.1"
                     /translation="MLRETLRKVADGESLTEGEAERALETIMEGNAPPEATAALLTAL
                     RVKGESVPEIVGFARAMRRFAAKVEAPEGVVDTCGTGGDAKGTINVSTAAAFVARGAG
                     VVVAKHGNRAATSRAGSADVLEALGAEIDLGPDQVSRCIADAGIGFMFARTHHPAMRF
                     VAPVRAELPFRTVFNLLGPLTNPAGARRQLVGVFGGGYVRPVAEALRDLGAEKALVVH
                     GTDGMDEITVTDKTLVADVSEGEIEEYEISPEDFGLSRHAPDGLLGGDAHLNARILRD
                     ILSGEEMGAACDIVLLNAGAAIHVSGKAATIEEGVSLARESI"
BASE COUNT         1583 a         2918 c         2933 g         1524 t
ORIGIN      
        1 gccgagcagc gggtggacaa gagcctcgaa ggccgcgcta atcaggaaga ccgtccccac
       61 cgcagcagcg ccccatccga gactgtacaa ctcgagtggg acgagcacgg caaggccgct
      121 gaagagcagg ggagagagcc ctataaacca gaggcttgtc aggagcctcg gctgccacac
      181 cgccctgagg gtcggcatca gggatacgta ccctgaagta gccggtgccg gctccagtgc
      241 ggcccagatg gcgatgaccc cgcccgctac cgccacggac gcgaaggccg gaagcaggcc
      301 gaccgtggct gcgacgctcc cgaggaccgg gccaaggatc gccccgacca cggcagcgct
      361 gatcatcacc ccgatcatct cggcacgcct ttcttcaggc gcccggccaa cgagccaggt
      421 gaacgcggcg acccacgaga gcgcgctccc gaaaccgccg acaaaccgcg cagcaaccag
      481 cagcccgctc gtgtcaacga gcgcgaacat cacgctggtg atggacaaca ggatcaaccc
      541 gacgagggcc gtgatcctca cccctgagcg cgaagccagg tatcctccag gcgcactgcc
      601 taccagtacg ccggccccgt atgccccgct caagaggccg acagcggact tcgagaggcc
      661 gaactcctgg ttgaagtaag ggacgagcgg cgtcagagca gcgtagaaga tcgtgtccac
      721 aagcactatc gcgcagctga gggcaaggag tcgacgcacg gcggttattc taatacctga
      781 gttcgaagtc cgagccggaa tcgtttaagg cgtattgtgc cagggctgct aacatgttcg
      841 tcgtgaagac catctcgacc attattaccg gctggctcgc gtgggagagg cggagccagg
      901 gaaacaagac gcttttcgtc ctgtcgctct cgctggcggc gtttgtgacg ctggccttcg
      961 cctgggcgtc gctcaaccct gatcctggca acctcgcgcg ctacttcgcg ttctactggg
     1021 tgtgggggct atgcgctctc ggggtcctcc tcgggatggc cgagtacaag acgagcccgc
     1081 tacgcaagcg cagggaacgc ggccacctct tcatcctcct gtacatcggc gcgctgctca
     1141 tcgtgacccg cgcattgaac atggtcctct tctacggtta gctgtatgta cagggcactg
     1201 ctcttcgacc tcgacggcac gctcgcggag accgactccc tgcacctgcc gacgtgggtg
     1261 gatgcgctcg aaccgtacgg cgtcgatgtg gacgaggagt tctacagaga gaggatcagc
     1321 ggcaggagca cgggcgagat cgtacgagat ttgcttccag acctcaccga cgatgagggg
     1381 gaatccatag gtgacgccaa ggaggctagc ttccgcgagc gcgccgccga actggaaccc
     1441 ttaccaggtc tcgtggactt catagatcaa ggcagggagc ggggaatgaa gatcgcgctc
     1501 gtcaccaacg ctccaaaaga gaacgtcgag gcgatcctgc gctccctcag gctgcaagag
     1561 tccttcgatg tggtagtgct cgcagatgac gtggaggccg tgaagcccga cccggccccc
     1621 tacagagccg ctctgaagaa gatcgacgtt cccgcggaag aggccatggc tttcgaggac
     1681 tctatctcgg gcatttcctc ctccgtcgca gcaggcatcc cgacagtagg tatcgcttcc
     1741 acccaggacc cggagaagtt gatcggcgcg ggagcattta tgacggcgaa ggactttacc
     1801 gaccctgatc tgagatccat gatcggcgca taggggcggt ttgcccccgg ctgcttcttt
     1861 cgagaaacgc ccgcaacttc agcgcatact cgggatgctc gtaagcttcg acgtgtccgt
     1921 aaccttccag cttccagagg ctcgcctcgg gaccggcctc aacgaggatc cgggcctgct
     1981 cgtagggtac gatgtcgtcc gcggtcgagt ggatgatgag gagcggcacg ccttcgtctg
     2041 agagcttcgc ggcgtcgacc ttcggtacaa cttcccaggg gtcgaagtcg gggaaaaggc
     2101 ttccagccag caggatcgca ggccggagca tcctcccgaa gggtatgaac tcgggtattt
     2161 tgactttgag aaggagcggg aggtcaccgt atccagcctc ctcgacgacc gccgccacac
     2221 cggtccccgg ggccgcacgt agggcggtcg agccccccat ggaccagccg tgaagcacca
     2281 cgtcttctgc cttgtagcct tgctgatcta gccaggcgag cgcgccgcgc acgtcacgca
     2341 tctctctgta gccgagggtt cggcgcgccc cgctcgactc tccctgcgcc cgcaggtcca
     2401 gcaggagcac gccgtagcct gcgttgtggt agacggggac ggtccgcagc aatttctcat
     2461 caaacttgta cccgccccag ccagggacga gcacggcggc cctggacgag tcctcagcgg
     2521 gcacccacca ggcgctcagg tttaccccgt ctgtgctctg gatctcgacc tccctgtatc
     2581 cgagcccgac ggaggcaggc gtcgcctcag gtcgtttgcg tcgcggggag gtcatcaata
     2641 gcaccacgag aagtcccacg ccaatgtacc cgagggacag agtgacaagc aggacgagcg
     2701 cacggcggag cgttcttctc atccctccga cattgccgat ggcgatgcta aaggatcacc
     2761 tccaccgtca caggaccttc ctcttcgccg agcgagacgg tgagcacgtt ctccccaaga
     2821 tccggctctc gtgcctcgcc attgaccgtg acgccctgcg ccgccgtgac gccgcgtagc
     2881 tccagcctta cctctccgcg ctcaggggcg aacgaaccct cgcgctcgcc gaggcggatg
     2941 gtaatgcggt tgtccgagga ctcgcaggag atcgtacgcc gggcgtactc cccggcttcg
     3001 tagccgaatc cgtctccggc gtcctcgtag atggtggatt ctccggcacc ctcggcaggg
     3061 aagacgagta gcgtcagggg gtcggtcggc ctctcgccgg tgtgcgcggc gtcggggccc
     3121 atcggggcgg gagtattcgc cttgacgtag atggctggct cgccgagggg cgcgtgcgcc
     3181 aggacgtggg cggggccttc gaagcgctcg ccgctccagt agtggaacca tgttccctcg
     3241 ggaaggtaga cgtgacggtg ctcgatgccc gggcgcgata tcggggctat gaggagggcg
     3301 tcgccgagca ggaactcgtc gtcggcggag taggtagtct cgtcgtcggg gtactcgaag
     3361 aggagtgggc gcaggatcgg cgccccggtg cggtggcact cgtcgaagag ggagtagaag
     3421 taggggagca gccgctgtcg gagtttgatc atcttcctgc acaccgactc gcagggttcg
     3481 ccgaaggccc agggctcctg gtggcgcgtc cccagcatcg tgtggttgcg gcagaaaggc
     3541 tggaagaccc cgaactccgt ccagcgggcc aggagctccg cgttcgtgtc gcccccgaac
     3601 cccccgatat ccaccccggc ccaggctatg ccggagaggg cgaggttctg tagctggggc
     3661 atgctcatcc acaggtgctc ccaccatgag gagttgtccc ccgtccattg catggcgtga
     3721 cgctgcaggc cggcgtaccc ggcgcgagtt atgacgaaag gccgctcgtc tggccgcagg
     3781 gcaagcaggc cctcgcgggc tgcgcgggcc atcagagaac cgtatgtgtt gtggatctgg
     3841 gcgtgccact tcgcctctcc ggtccgtccg ccgggatgta ccacgtcgtc aggcatcgtg
     3901 gccttgggta tgaacagcgc gggctcgttc atgtcgcacc agatcccggc gaccccttca
     3961 tctgtgagag ccgactggtt ctcgccccac cacctgcgtg tatccgggtt ggtgaagtcg
     4021 gggaaggcgc agacgcccgg ccagacggcg ttgtggtact cctcgccgtc atgagtcttg
     4081 cagtaaaggt ccttctcccg tccatcggta tagacggagt agttctcgtc taccttcacg
     4141 ccggggtcca cgatggtgac tacgcggaag ccttgctctc tcaggtcgga gatcagacgc
     4201 tgcgggtcgg ggaagcgctc tttgttccag gtgaagacgc gaaagccgtc catgtagtgg
     4261 atgtcaaggt agatgacgtc gcacgggatg tcccgctcgc ggaagccgcg ggcgacctcg
     4321 cgtacctcct cctcgttcat gtaagtgtag cggcactgct ggttgccgag ggaccacatc
     4381 ggcggcatcg gggtgcggcc tgttagctcg gtgtatcggt cgacgacgtc ccgcggtgtc
     4441 ggcccgcaga agacgtagta tacgatcgcg ccaccctcgg ccccgtagta ggcgcggtcc
     4501 tcatcctcga gcgccaggtc gaactcgacc ctgtgggtgt tgtcgaagaa gagtccgtgc
     4561 gccttgccat tggtcatcga gaacgtgaac gggatggagc tgtacaggtt gttgaaggag
     4621 gcggtgtgcc cgagcggcgg gtccacgttg tagaagacct ggtgcgagcc ggtcttctcg
     4681 agcccgctcg tccgctcgcc gcagccgaag tacctttctc cttcctcccg cttcttgtag
     4741 agccgcaccg gactgccgag gggctcggag aagatgtcgg ccccaggagt ctcgacggcg
     4801 cccatcccca gctcttcgtc gtccaccgcg aactgcctgc cggaatggtc gctgaatcct
     4861 acccgcagcg gacgcagcga gacgcgcgcc gttgctgccg tggtggagag cgtgagctcc
     4921 tcgtcggact cctgcatcga gacctcgacc gtctcccaat cctccttcgc gattgcctcc
     4981 gagtcgtacc tcggcgggcg gcccgcgggg aacatcccga cccggaagag gtcaggggcc
     5041 agggccgtca cttcgacggt cgtcgaccct gctctcaccc gcaggccgcg gtcgtcgtgg
     5101 tcgagcagct ccccggtgcc gagcggctcg tagttgcccc ctgtctcggg ctgcttctcg
     5161 taagtctcgg acaatgttcc tccccgcggt cggaagccgg tcagctcttc tgcatttcag
     5221 cttgtacgct atttggttga ggatggcatt gtaccggact attttgcttt gccgcccccg
     5281 gcactcacgg ctccggcgag gaggatgacc acggtgctca gggccacgac gaggtagagg
     5341 gctgctccga ggctggtcca ttccgcgacg aacccgatga acggagggcc gacgaggaag
     5401 cctgtatacg ccgtggtcga gacggcggcg agggcgggac cggcggccat gcccttcgtc
     5461 cgccccgcgg cgctaagggc gaccgggaag actatggaga aacctgctcc ggcgcaggcg
     5521 aagccgacca gcgcgacgag aggttgcccg acggccagcg cgaccccgag ccctatggcc
     5581 gacaggaccc cgcacaaacg gacgagcctc gtggggccga gtcgctctgt aaaccggtca
     5641 ccgaagaggc gtccgaacgc catggcgagc gagaacgccg cgtatcctgc tgcggcgaag
     5701 ccggggcctg tctggagggt attgttcagg tagaccgcgc tccagtcgcc catcgcgccc
     5761 tcccccagca ggacgcagaa cgagatgatc ccaagcccca tcagagcccg cgtcggcctg
     5821 gcgaacgccg gcgccccgtc ttccgaagcg tctgcgccgg ccggcaacag cttcttgtag
     5881 acggcggcga aggctatcgc ggaaaggact gctatggagg cgaagtgtgg aagcgggccc
     5941 atcccgaggg aagcaaagag tcctcccacc gccgcgccgg cgagccctcc gaagctccac
     6001 gcagcgtgaa atgaattcat gatcgggcgc ccgtagccct cctcgacggc gacggcctgc
     6061 gcgttcatgg agacgtcgag cccgccgttg gcggccccga tcatcgcgag agcgggaacg
     6121 aggaggatca tactcggggc cagggcaggt aagacgagcg agagggagag cagcatcgcc
     6181 gtcgtcccga ccacacggcg gctcccgaat cgggagacga gggctcccac gagcggcatg
     6241 gcgacgagcg ccccgacagc ggccccgagg agtgcgaggc cgagctggcc ctcgctaagg
     6301 tcgagctcct gctgaatcgc gggtatcctg acgacccagg tcccgatcac gacgccgttg
     6361 acgaagaaaa cgcctagcac agcgaaccgg ggcacgctca gagcaagttg cggtccgttg
     6421 gcgaacacta gaagtctatg tccctgccga cgtcgcgctc caccgcgcgg tcgtagacga
     6481 cgcgggccgc ggccatgtcc tctagcgcga gtccctggct ggcgaagagg gttatgtcct
     6541 cggggccgcg acggccaggc acctggcctg cgatcacctg tcccaactcg tagacggcct
     6601 cagggaagat ggcgcccgtt tcgagggaca gcatcaggtc tccggcctcg agaccaagct
     6661 cctcgcgtga gtcggcgcac acgaagctcg cccgcctgac gacctcgcgg tcgatctcgc
     6721 tcttgaacag gaagttggac cccgcggcgt tgacgtgcgc cccgggcctg agccactcgc
     6781 cgaggagcac gggttccctc gatgaagtca cggtgacgac gatatcctgg gccgctggct
     6841 cctcggcggc gtgggtggtc tctatctcca tcccgagcct tttgcccatc ttctcggcga
     6901 acgcctttcg cgaatcctcg cggcggctgt atacgatgac cctgtcgagg tctcgaacgg
     6961 atgctatggc ctctagctgg ctctccgcct gccaacctgc gccgtagatg cccaaagtgt
     7021 tcgcgtcctc acgcgcgagg tgcttcgtcg cgacgccgct cgcggcgcct gtacgctgct
     7081 gcccgagcct gtcggactgc atgatcgaga gcagctcccc gccctcaggg tcgaagagca
     7141 ttgtgtagaa gcgtgcgccg gtgcgggcga cggtatatgc cttgagacct agagccccga
     7201 tctccggcgc gcccgcggcc atgtagttga ggccgcccgt cgggagggct acgcggcgtc
     7261 tcgcgcggtt ggtggcgcgt ccctcggcgt gctggcgcag gaccgcctct acggcatcca
     7321 gggtcgatgg catgtcgagc agcgattcga cctcgttctc ggtcaggaac agggtcaagg
     7381 cgtctcctcc ctgcttgccg gcggcttatc cgctaaaatc gctccactta tgcgggtcct
     7441 tgtaatagac aactacgact cctttacgta caacctggtc cagtatctcg gggagctcgg
     7501 ggccgaggtc ctcgtgcgcc gcaacgacga ggttataccc gaagaggtct ccgaactcca
     7561 tccggatagg atagtagtct caccaggtcc ctgcacaccg aacgaggcgg gggtctctgt
     7621 cgaactcata gagaaggtct cgaacgaggt tccgatcctc ggcgtctgcc tcggccacca
     7681 gtccataggg caggcgttcg gcgccaaggt cgtgcgcggc gagcccgtac acggcaagac
     7741 ggcgaggata atgcacgacg gcgaaggtgt ctacaggggc ctggatcagg gcttcgtggc
     7801 gacccgctac cactccctcg tcatcgaccc ggagtccgtg cctgactgcc tcgtcgtcac
     7861 cagccgcacc gaggacggta cgatcatggg cgtcaggcac cgcgagtacc ccgtagaggg
     7921 cgtccagttc caccccgaga gcgtcctgac ccgcagcggt cacgacctcc tcaagaactt
     7981 cctggggctc tgagccaacc ccttgctgcg cgagacgctt cgcaaggtcg ccgacgggga
     8041 atccctgacc gagggcgagg ctgaacgcgc gctggagacc atcatggagg gcaacgctcc
     8101 ccccgaggcc acggcggctc tccttactgc gctgcgcgtc aagggcgagt cggtacccga
     8161 gatcgttggc ttcgcccgcg ccatgcgccg cttcgccgcg aaggtcgagg cgcctgaggg
     8221 ggtggttgac acgtgcggga cgggcggcga tgccaagggc acgataaacg tctcgaccgc
     8281 ggcggcgttc gtggcgcgcg gcgcgggggt cgtcgtcgcc aagcacggca atcgcgcggc
     8341 gaccagcagg gcagggagcg ccgatgtatt ggaagcacta ggcgcagaga ttgacctggg
     8401 gccggatcag gtctccaggt gcatcgcgga cgcgggcatc gggttcatgt tcgcccgtac
     8461 gcaccaccct gctatgaggt tcgtagcccc cgtgcgggcc gaactgccgt tcaggaccgt
     8521 tttcaatctg ctcggcccgc tcaccaatcc ggcgggagcg aggcgccagc tcgtcggggt
     8581 cttcggcggg ggctacgtgc gtcctgtcgc cgaagcgctg cgggacctgg gtgccgagaa
     8641 ggcgctcgtc gtgcacggta ccgatgggat ggatgagatc acggtcactg acaagaccct
     8701 cgtcgccgac gtctcggaag gagagatcga ggagtacgag atctcaccgg aagacttcgg
     8761 cctctcccgc cacgcccctg acggcctcct cggcggcgac gcccacctca acgcccgcat
     8821 ccttcgcgac atcctctccg gcgaggagat gggagcagcc tgcgacatcg tcctcctgaa
     8881 cgccggagcc gcgatccacg tctcaggtaa agcagcgacg atagaggaag gcgtaagtct
     8941 ggcacgggag tccatcag
//
LOCUS       DATAIR010000769         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_75398, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000769 DATAIR010000000
VERSION     DATAIR010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75398"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..2195
                     /locus_tag="VHM69_18270"
     CDS             207..2195
                     /locus_tag="VHM69_18270"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2742391.1"
                     /translation="MAENLVGKILKEHMIEGALDPGDEVGLRIDQTLLQDATGTMACL
                     QFERMGVDRARVDFAVQYIDHNVIQLDFKNPDDHRFLQAFAARYGIHYSRPGNGICHY
                     LHVERFARPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQTPAPKVVGVE
                     LTGALPQWVTPKDVILELLRRRGVRGGLGRIFEFYGEGVANIDVTGRTTICNMIAELG
                     ATTGVFPSDERTKEWLRMQQRPDDFVELSADEGADYDEREHIELDKLEPLIARPHSPG
                     NVVPVSEVAGTRAAQVCIGSSVNSGFEDLAVAAAVLRDETVYPGLVMTVTPGSRQILD
                     SIAQTGVYSDLVRAGARMLEPVCGPCVGMGQAPPSDSVSVRTFNRNFPGRSGTMTDQV
                     YLTSPTTAVATALKGVITDPRELGDYPDLPDAPANPAVDDRQILAPAPPEEASGIEIV
                     RGPNIKPPPEAPELPESLECTVTIVVGDDISTGDLAPDGVEVMSFRSNVPEIAKFTFR
                     RFDPEYHKKAQEMAPGFIVGEHNYGQGSSREHAALAPLHLGIRAVIAKSFARIHRRNL
                     ISQGILPLRFKDESDYDTFEEGQEWELQEIRKRLENGDEEIPLRKEDDEVTLLAEYSQ
                     RERDILLAGGILRQLREKQGASVSKGEPAEPEHRGRDH"
     gene            complement(2279..2827)
                     /gene="lspA"
                     /locus_tag="VHM69_18275"
     CDS             complement(2279..2827)
                     /gene="lspA"
                     /locus_tag="VHM69_18275"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2742392.1"
                     /translation="MLGSNADEYPDAFFIQMMSRRTGLVLALALALVVFFVDQWIKSV
                     VEGSMRVGESITLVPGFLSLTYIKNDGGAFGILGGSQVLLLVGSAIAVVVVLWMLLSG
                     RPTKPTMLGCGLILGGAAGNLLDRLTAGEVTDYVHFSFWYIFNAADAAIVVGVGLLLL
                     STLRPERSGSPLRGPAPKSEDG"
     gene            complement(2840..3310)
                     /locus_tag="VHM69_18280"
     CDS             complement(2840..3310)
                     /locus_tag="VHM69_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742393.1"
                     /translation="MRRSHDETGTKGEAHPVFSALYGPLGFCIERLWMGERRARLLKG
                     ARGAVLEIGGGAGANLPHYRDVDRVTVAEPDPYMRKRLVRKLGATGSTARLQDRIEPF
                     WRRLLGGCHPNRDTVAAIEDAWFEIESFESFYPPVPLSGLIPHVQGSATVKLAA"
     gene            3469..3891
                     /locus_tag="VHM69_18285"
     CDS             3469..3891
                     /locus_tag="VHM69_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX2742394.1"
                     /translation="MEQNDHNYGVAEERGPATMSTQEERTWSVLAHLSIYLNLVTGFL
                     GPVAALFIWLVYRDRSRKVAFQALQSMWYQVAWLVILVSGWTVTTLLMLVLIGFLLVP
                     VMALITVVPFVHSAYAAYRVSKDGDYRYPLIADMIEAR"
BASE COUNT          750 a         1241 c         1281 g          709 t
ORIGIN      
        1 atcgccatga atctcatgca tcttgcgtgg ctgcccgagt tctctggggt ccatatctgg
       61 tgtagatcag catgccatta ggggagggca catcactcga acggaccatt tccggtacat
      121 ccgatccagc ggaactcggg ctggcccaga agcgcaggtg tgtagaatag gcgagcaggt
      181 cagggaaagt aagtcggggg tgtgccatgg cggagaacct tgtcgggaag atcctcaaag
      241 aacacatgat cgagggggcg ttggatcccg gcgacgaggt tggactgagg atagatcaga
      301 cactccttca ggatgcgact ggcacgatgg cctgcttgca gttcgagcgg atgggcgtcg
      361 accgcgccag ggtggacttt gccgtgcagt acatcgacca caacgtcatc caactcgact
      421 tcaagaaccc ggacgaccac cgtttcctgc aggccttcgc cgccagatac ggcatccact
      481 actcccgccc tggtaacggc atctgccact acctgcacgt cgagcggttc gccagacctg
      541 gcgcgacact cgtcggggcg gattctcata cgacttcctc cggcgctctc ggctccatag
      601 ccataggggc cggcggcctc gacgtggccc tcgtgatggc cggccagccg tttcagaccc
      661 cggctcccaa ggtcgtcggc gtcgagctta ctggagctct ccctcagtgg gtgacgccga
      721 aagatgtgat cctggagctc ttgaggcggc gcggcgtgag gggcggcctg gggcgcatct
      781 tcgagttcta tggagaaggc gtcgcgaaca tagacgtaac cggacgcacc acgatctgca
      841 acatgatcgc cgagctcgga gccaccaccg gcgtcttccc ctcggacgag aggacgaaag
      901 agtggctgcg gatgcagcag cgccctgacg acttcgtgga actctccgcc gacgagggcg
      961 ccgactacga cgagcgcgag cacatcgagc tcgacaaact cgaacccctg atcgcacggc
     1021 cccactcgcc cggcaacgtc gtacccgtga gcgaggtcgc gggtaccagg gcggcgcagg
     1081 tctgcatcgg tagctcggtc aactcgggat tcgaagatct tgccgtcgct gccgcggtcc
     1141 tcagggacga gacggtgtat ccggggctcg tcatgaccgt gacgcctggg agccgtcaga
     1201 tcctcgactc catagcccag acaggcgtct attccgacct cgtgcgggcg ggcgctcgga
     1261 tgctcgaacc ggtgtgcgga ccttgcgtcg ggatgggaca ggctccgcca tccgattcgg
     1321 tgagcgtcag gacgttcaac aggaacttcc caggacgctc aggcacgatg acggaccagg
     1381 tgtacctcac gagccctacc acggctgtcg cgacggctct caagggcgtc atcaccgacc
     1441 cgcgcgaact gggcgattac ccggatctcc ctgatgctcc cgcgaatccc gcggtggacg
     1501 accgccagat cctcgctccg gcgccgcctg aggaggcgtc gggcatcgag atcgtgcgcg
     1561 gccccaacat caagccgccg cccgaggccc ccgagttacc tgagtcgctg gaatgcaccg
     1621 tcaccatcgt ggtcggggac gacatctcga ccggcgacct ggcgccggac ggggtcgagg
     1681 tcatgtcgtt ccgctcgaac gtgcctgaga tcgcgaagtt caccttccgc cgcttcgatc
     1741 ccgagtacca caagaaggcg caggagatgg ctcctggctt catagtcgga gagcacaact
     1801 acgggcaggg gtccagccgc gagcacgcgg cgctggcacc tttgcacctc ggcatcaggg
     1861 ccgtcatagc caagagcttc gcccgcatcc accggcggaa cttgatctcc cagggcatcc
     1921 ttccactccg cttcaaggac gagtcggatt acgacacatt cgaggaggga caggagtggg
     1981 aactgcagga gatccggaag cgcctcgaga acggtgacga agagatcccg cttaggaagg
     2041 aggacgacga ggtaacactc cttgccgagt actcgcagcg ggagcgcgac atccttctcg
     2101 ccggcggcat cctcaggcag cttcgcgaga agcagggggc gtccgtcagc aagggcgaac
     2161 ccgcggagcc cgagcatcgc gggagggacc actaatccac aaaggtcgga ggtctcatga
     2221 gaagagcctg gtggatcgtc tcgcttcgca atgggcttgt gccaggttct aattgactct
     2281 acccgtcttc agatttcgga gcgggtcctc gaaggggact tccagacctc tcgggacgga
     2341 gggtggagag cagcaggagc ccgacgccga cgactatcgc tgcgtcggcg gcgttgaaga
     2401 tataccagaa gctgaagtgg acgtagtctg tgacttctcc tgcggtcagg cggtcgagga
     2461 ggttgccggc tgccccgcca aggatcagac cgcaccctag catcgtcggc ttcgtcggcc
     2521 tccccgagag cagcatccac agcacgacga cgacggcgat ggcgctcccc acgaggagga
     2581 gtacctggct cccgccgagg atgccgaagg ccccgccgtc gttctttatg taggtgaggc
     2641 tgaggaagcc gggcacgagg gtgatgctct cccctacccg catcgagcct tcgacgacgg
     2701 atttgatcca ctggtccacg aagaagacga cgagcgcgag cgcgagggcc aaaaccaggc
     2761 ccgtcctgcg gctcatcatc tggatgaaga atgcgtcagg gtactcatcg gcgtttgatc
     2821 ctagcatacc ggcctattct caggcggcca gcttgacggt cgccgagccc tggacgtgcg
     2881 gtatgaggcc ggacaggggg accggaggat agaagctctc gaacgactct atctcgaacc
     2941 aggcatcctc gattgccgcg acggtatctc tgtttgggtg gcatccacct aagagccgcc
     3001 tccagaaggg ctcgatgcgg tcttgcaagc gggccgttga gcccgtggcc ccgagcttgc
     3061 ggaccagcct cttgcgcatg taagggtcgg gttcggcgac ggttaccctg tccacgtctc
     3121 ggtagtgtgg gaggttcgcg cctgcgccgc ccccgatctc gagcacagcg ccacgggccc
     3181 ctttgagcaa ccgcgccctg cgctcgccca tccatagtcg ctcgatgcag aagcccagcg
     3241 ggccgtagag cgccgagaag acgggatgcg cttcgccctt ggtgcctgtt tcgtcgtggg
     3301 atctccgcat tagctctcct gatggagtct gccatgctcg gggcttctcc gccatccgag
     3361 aagctgggac tgcggaccta ccgacattcg gtcccgcgac cgatgccgaa agctctgtcc
     3421 gggggtattt tctcgatata gcgtatcgag caacagcgag gagaagagat ggagcaaaac
     3481 gaccacaatt acggagttgc cgaagagcgg ggtccggcga cgatgtcgac gcaggaggag
     3541 aggacctggt cggtgcttgc ccacctgagc atctacctca acctggttac agggttcctc
     3601 ggaccggtgg cggccctgtt catctggctg gtctacaggg acaggtcgag gaaggtggcc
     3661 ttccaggctt tgcagtcgat gtggtaccag gtggcctggc tcgtaatcct ggtctcagga
     3721 tggaccgtga cgacgctatt gatgctcgtc ttgatcgggt tcctgctcgt ccctgtgatg
     3781 gccctgatca cggtggtccc cttcgtgcac agcgcgtacg cggcctaccg cgtgagcaag
     3841 gacggagatt accgttaccc gcttatcgcc gacatgatcg aagcccgcta gacggctctg
     3901 acggaaccgg cgcctccgac ctcgtcgggg gcgccgctcc aggcgggtgg tctgacgcca
     3961 ttcgcacacg tcgtgcgtat g
//
LOCUS       DATAIR010000770         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_361642, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000770 DATAIR010000000
VERSION     DATAIR010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361642"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VHM69_18290"
     CDS             complement(<1..847)
                     /locus_tag="VHM69_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2742395.1"
                     /translation="MGSMLAVVVEPEAVGRLSLGEVEEPDPSPSEALVRVEAISLNRG
                     EVRRAETNEPGFRPGWDLAGTVERAAEDGSGPAEGVRVVGFLPSGGWAELAAVPTNAL
                     AELPDGVSFEEAATLPVAGLTAMYALEEGGNLVGRSVLITGASGGAGLFALQLARISG
                     ARVVALVRREEHVEMVTEAGAHEVVVSEDGGAAREHAPYGLILESVGGEVLGNALSML
                     ATGGTCVSFGISGGTEVTFDARTFYFTGGARLYGFILFHEVLAKPASVGLARLVRLLD
                     EGKLRP"
     gene            926..>1731
                     /locus_tag="VHM69_18295"
     CDS             926..>1731
                     /locus_tag="VHM69_18295"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX2742396.1"
                     /translation="MQYATIKNLPENVDLMVLDASGTLREDVGLEELPEHLADKDSLV
                     WCDIYSTQGGQSGPYGRLLREVFEFDELTVEDCFTRSHLPKVDVYDDYLFVALFSFHL
                     SEKTRRVQTVEVDMYVGENYVVCVHPRPLRELDRVRRRLLAGDDFVASSPANVAHTVF
                     DAVVDEYLPIMNRLSAMVDGIEDDLLGEGEEAPPDILDSLFHLKHELTALRRLAVPLR
                     DVIGILLRPTTRLIPEESRMHYEDVRDHLFRVVDMIDTMRDYLAGSLDIYT"
BASE COUNT          305 a          618 c          513 g          295 t
ORIGIN      
        1 gtggtctgag cttgccttcg tcgagtagtc tcacgagccg ggcgagcccc acggaggcag
       61 gcttcgcaag cacctcgtgg aacaggatga aaccgtagag cctcgccccg cccgtgaagt
      121 agaaggtccg cgcgtcaaac gtgacctctg tcccgccgga gatcccgaac gagacgcagg
      181 tcccgcctgt ggcgagcatc gagagcgcgt tccccaggac ctctccgcct acggactcca
      241 ggatcaggcc gtacggcgcg tgctccctcg ccgcccctcc gtcttcactg acgacgactt
      301 cgtgcgcgcc ggcctcggtg accatttcca cgtgctcctc gcgacgcacc agcgcgacga
      361 cccgcgcccc gctgatgcgg gcgagttgca gtgcgaagag cccggcaccg cccgatgcgc
      421 ctgtaatgag cacggatctc ccgacgaggt tgcctccctc ctccagggcg tacatggccg
      481 tgagaccggc cacgggcagc gtggccgcct cctcgaagga gacaccatca ggcaactcgg
      541 ccagcgcgtt cgtcggaacg gcggcgagct ccgcccatcc tcccgagggc aggaacccca
      601 cgacccggac gccttctgct gggccggagc catcctccgc ggcccgttcg accgtcccgg
      661 caagatccca gccggggcgg aatccgggct cgttcgtctc cgccctacgc acctcgccgc
      721 ggttcagcga gattgcctcg acccgcacca gcgcctccga gggcgaagga tcaggctcct
      781 cgacctcacc cagagagagc ctgcccacgg cttcaggttc cacgacaacg gctagcattg
      841 atcccatcgt tctcctttct ccatcggcat cattgcagtc tacttcacgg gcttcgggac
      901 cgggcgtccg ctgtataatc tgcccatgca gtacgccacg atcaagaacc tgccggagaa
      961 cgtcgacctc atggtcctcg acgcatcggg cacgttgcgc gaggacgtgg gccttgagga
     1021 gctccctgag cacctggcag acaaggattc gctcgtctgg tgcgacatct acagtaccca
     1081 gggcgggcag agcgggcctt acggacggct cctgcgcgag gtcttcgagt tcgacgagct
     1141 cacggtcgag gactgcttta cgaggagcca ccttcccaag gtcgacgtct acgacgatta
     1201 cctcttcgtc gcgctcttct ccttccacct ctccgagaag acccgccgcg tccagacggt
     1261 cgaggtggac atgtacgtcg gggagaacta cgtcgtctgc gttcacccgc gcccgctgcg
     1321 ggagcttgac agggtaaggc ggaggctgct cgccggagac gacttcgtcg cctcctcgcc
     1381 ggccaacgtg gcccataccg tcttcgacgc cgtcgtggac gagtacctgc cgatcatgaa
     1441 caggctttct gcgatggtcg atggtatcga ggacgacctc ctcggagaag gcgaggaggc
     1501 gccgcccgac atcctcgact cccttttcca cctcaagcac gaactgacgg cgctcagacg
     1561 tctggcagtg cccttgaggg acgtcatagg catcctcctc aggccgacca cccggctaat
     1621 ccccgaggag agccgcatgc actacgaaga cgtgcgcgac cacctcttca gggtggtaga
     1681 catgatcgac accatgcgcg actacctcgc cggctccctg gatatctaca c
//
LOCUS       DATAIR010000771         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_268615, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000771 DATAIR010000000
VERSION     DATAIR010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268615"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            563..1411
                     /gene="arsM"
                     /locus_tag="VHM69_18300"
     CDS             563..1411
                     /gene="arsM"
                     /locus_tag="VHM69_18300"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX2742397.1"
                     /translation="MDEGLKQRVRERYAEAALSVLGEAEDSSSCCGGGASCCGVAESE
                     ERAALEVDMVCGSYSEAERDELPRLAAEASLGCGNPVALATLSAGEVVLDLGSGGGID
                     VLLSARRVSPGGKAYGLDMTDEMLELARRNQAEAGVENAEFLKGEIEAVPLPDDHVDV
                     VISNCVINLSTEKPRVIGEAFRVLRPGGRFAVSDVVFLGEKSRLPASVVRSVELWSGC
                     ISGALEKSEYERLLSQAGFEEAAVEVTHTYPPEQIAGQSGEDVEALRAVPVASAFIRG
                     RKPELR"
     gene            1465..1833
                     /locus_tag="VHM69_18305"
     CDS             1465..1833
                     /locus_tag="VHM69_18305"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2742398.1"
                     /translation="MATDDAGEERIGRLVALGRALSDPIRVTMIGMLADGRGCCDLPN
                     CGVPAVDQDAGICVCEFERYFDVGQSKVSYHLGKLKEAGLVREERRGKWSFYSLDRQA
                     AGQLLSETAAHLDGGRSKDG"
     gene            1826..>2265
                     /locus_tag="VHM69_18310"
     CDS             1826..>2265
                     /locus_tag="VHM69_18310"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX2742399.1"
                     /translation="MDSRKSGDRLRVLFLCTHNSARSQMAEGLLRDMAGDRFEVFSAG
                     TEATLVRDEAISVMAEIGVDISAQESKTLERYISEPFDLVVTVCDDANEACPVFPGAR
                     DRLHWSFPDPSRVKGEERLAAFRKVRDEIQARIEVELANEEARA"
BASE COUNT          430 a          594 c          786 g          455 t
ORIGIN      
        1 agcctcgatt aggctatgcc gagcatcgtg ttctggttct tctgcatctt ggccgaagag
       61 gcgtatgtgc cactcatcag ttgttaatcg aagcgccggt agttcatgtt caagcttttg
      121 tgctagcgtt gttttcccag agcaaggaag cccaactatc aaatggagag tagccacgtt
      181 tcttactcct aaaggtcatc tagaagctgc ccgactcgtt atcatacgga cgcactccgg
      241 ataatgcttg ctcggcggtc ttatgcggaa ctgcgtgagg ctgttctggc gagaagtata
      301 accggtgggg gtagcaagcg accgaacacc cgaatcgagc gggcgaatgt cgtgcgagtc
      361 gccaggaatg gtaatgccac agcgagggta cacggtcgag cctgtcgcgg atcctgacga
      421 ccccggcggt taggctgtct cccgccaatc ttgtgacagc catgtgtcgc atacattgtg
      481 agtcggtctg cctccactcc ttgacatatc gattttcttt gatctatact cacatcaaat
      541 gttcttgatg tgaggagtag agatggacga gggcctcaag cagagagttc gggagcggta
      601 cgccgaggcg gctttgagcg tgctgggaga agcggaagac agtagttcgt gttgcggggg
      661 cggcgcgtcg tgttgtgggg tggcggagtc tgaggagcgg gctgcgctcg aggtggacat
      721 ggtatgtggg agctattcgg aggctgagcg ggatgagttg ccgcggctcg cggccgaagc
      781 ttcgctgggg tgcggcaacc cggtggcgct ggcgacgctc tcggctggcg aggtggtgct
      841 tgatctcggg agcggtggcg ggatcgacgt cttgctctct gcgaggcggg tatctccggg
      901 aggcaaagcg tatggtctgg atatgaccga cgagatgctc gaactggccc gcaggaacca
      961 ggccgaggcc ggggtcgaga acgcggagtt cttgaagggg gagatcgagg ccgtgccgtt
     1021 gcctgacgac cacgttgacg tcgtcataag caactgcgtc atcaacctct ctaccgaaaa
     1081 accgcgtgtg atcggtgagg ctttcagggt cctgaggccc gggggccggt tcgccgtctc
     1141 cgacgtggtg ttcctcggcg agaaaagcag gttgccggcg agtgtagtgc ggagcgtcga
     1201 actctggtcc gggtgcatct ccggagcgct ggagaagagt gagtacgagc ggttgctctc
     1261 gcaggccggg ttcgaggagg ccgccgttga agttactcac acgtatcccc cggaacaaat
     1321 agcggggcag tccggcgaag acgtcgaggc tctgcgcgcg gtccctgtgg cgagcgcctt
     1381 tatccggggc cgtaaaccgg agctacgata gccgtggcag ggcggcaact gcatattctc
     1441 cagaaaaaga gtgttccgac ctcggtggct acggatgacg ccggagaaga gcgcatcggg
     1501 aggctcgtcg cgcttggccg ggcgctttcg gacccgatca gggtgacgat gatcgggatg
     1561 ctcgccgatg gacgcgggtg ctgcgacctt ccgaactgtg gcgttccggc cgtggaccag
     1621 gacgccggca tctgtgtctg cgagttcgag aggtatttcg acgtgggaca gtcgaaggtc
     1681 tcctaccacc tcggcaaact aaaagaggcc gggttggtgc gcgaggagag gcgcggcaag
     1741 tggagcttct actcactcga ccgccaggcc gcaggacaac ttctctcaga gacggcagcc
     1801 catcttgatg gcggcaggag caaagatgga tagccggaaa tcgggggatc gactgcgggt
     1861 cctcttcctc tgtacccaca actcggcccg ctcccagatg gccgaagggt tgctgaggga
     1921 tatggcgggt gaccgtttcg aggtgttcag tgccggtacc gaggcgacgc ttgtcaggga
     1981 tgaggcgatc tccgttatgg cggagatcgg ggtggacatc tcagcccagg agtcgaagac
     2041 gctcgagcgt tacataagtg aacccttcga cctcgtcgtc accgtttgcg acgacgccaa
     2101 cgaggcgtgc cccgtcttcc cgggagccag ggatcgcctg cattggtcct tccctgaccc
     2161 ctcacgcgtc aaaggggagg agcggctcgc agcatttcgc aaggtgcgag atgagatcca
     2221 ggcccgcatc gaggtcgaac tggcgaatga ggaggcgagg gcgtg
//
LOCUS       DATAIR010000772         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_361964, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000772 DATAIR010000000
VERSION     DATAIR010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361964"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VHM69_18315"
     CDS             complement(<1..607)
                     /locus_tag="VHM69_18315"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742400.1"
                     /translation="MLYRNFATQEELDAQYNLNVMFPDAASSYETFCERESEKVREDF
                     DHRLDVPFGPTLAEHVDLYPAGENAPVLVYVHGGFWYLRTSKEFGFVARGPVSGGVAT
                     VVTNYALCPRVTIDEIVRQTRAAVAWVHANAESFGGDPERIHVAGHSAGGHLVTMLLA
                     TDWEGEYGLPADIIKGATAISGLFDLAPFPFTFLQPKLQLTW"
     gene            complement(609..860)
                     /locus_tag="VHM69_18320"
     CDS             complement(609..860)
                     /locus_tag="VHM69_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742401.1"
                     /translation="MLLGIKYEAARIGEVGDEALAGIEEQMKEEIGESWESDDQVLRG
                     RNAVRREHGLPGLGEPGDRPPSPPATGARRPLWRRLLGR"
     gene            complement(914..2113)
                     /locus_tag="VHM69_18325"
     CDS             complement(914..2113)
                     /locus_tag="VHM69_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX2742402.1"
                     /translation="MIFEFSDKTREYRTRIEAFMDELVYPNEATFHKQLETAESRWSV
                     PPIMEELKQEAKTRGLWNLFLPDSDLGAGLTNLEYAPLCEVMGRSPIAPEAFNCNAPD
                     TGNMEVLVRYGTEEQKERWLRPLLDGEIRSCFAMTEPDVASSDATNIRARIQRDGDEY
                     VVKGRKWWTSGAGDPRCKISIFMGKTDTSAPRHEQQSMILVPLDTPGVEIERTLPVFG
                     YDDAPHGHAEISFNDVRVPIENIIWDEGKGFAIAQGRLGPGRIHHCMRLIGVAERSLE
                     MMCERVKERKTFGKPLAEQGVVQAWIADSRMEIEQARLLTLKAAHMMDTVGNKEARAE
                     IAMIKVVAPNVALNVLDRAIQAHGGAGVSGDFPLAYLWASARTLRLADGPDEVHRTSV
                     ARLELKK"
     gene            2055..>2403
                     /locus_tag="VHM69_18330"
     CDS             2055..>2403
                     /locus_tag="VHM69_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2742403.1"
                     /translation="MNASIRVLYSLVLSENSKITTSPLVRAAGPILAQWKGRRNRRGP
                     ASPVELALVKERKAICQEEVLDLGLEGKVALVTAASKGLGRAVATELAREGARVVISS
                     RDEDALSKTATEIH"
BASE COUNT          395 a          775 c          759 g          474 t
ORIGIN      
        1 cccaggtcag ctgcagcttc ggctgaagga aggtgaaagg gaagggcgcg aggtcgaaga
       61 ggccgctgat cgcagtggcc cccttgataa tgtccgcggg cagcccgtac tcaccctccc
      121 agtccgtcgc gagcagcatg gtgacgaggt gcccaccggc cgagtggccc gcgacgtgga
      181 tacgctccgg gtcgccgccg aagctctcgg cgttcgcgtg gacccaggct acggcggcgc
      241 gggtctggcg gacgatctcg tctatggtta cccttggaca gagtgcgtag ttggtcacca
      301 cggtcgccac gcccccggag acgggccccc gcgccacgaa cccgaactcc ttgctcgtgc
      361 gcagatacca gaagccgccg tggacgtaca cgagtacagg cgcattttct cccgcaggat
      421 agaggtcgac gtgctcggcg agtgtagggc caaaaggtac gtcgaggcga tggtcgaagt
      481 cttcgcggac cttctcgctc tcgcgctcgc agaacgtctc gtaggaagag gccgcgtccg
      541 ggaacatcac attcaggttg tactgggcgt cgagctcctc ctgggtcgcg aagttcctgt
      601 agagcacgct acctcccaag gagcctgcgc cagagcggcc gtctggcgcc ggtggccgga
      661 ggagaaggag gccggtcacc cggctctccg agtccgggga ggccgtgctc gcgcctgacg
      721 gcgttgcgcc ccctgagtac ctggtcgtcc gactcccagc tctcgccgat ctcttctttc
      781 atctgctcct cgatgccggc gagcgcctcg tcacccacct cacctattct cgccgcctcg
      841 tatttgatgc cgagaagcac ggagttcttg ctctggataa tcccctcctc tagtagttac
      901 ggccttcgcg ctgctatttc ttgagttcca gcctggccac cgaggtgcgg tggacctcgt
      961 cggggccgtc agcgaggcgc agggtgcgcg cgctcgccca caggtacgcg agcgggaaat
     1021 cgccggagac cccggctcct ccgtgtgcct gtatcgccct gtcgaggacg ttcagggcca
     1081 cgttcggggc gacgaccttt atcatggcta tctccgcgcg ggcctccttg ttgccaacgg
     1141 tgtccatcat atgcgcagcc ttcagggtaa gaaggcgcgc ctgctctatc tccatcctgg
     1201 agtcggcgat ccaggcctgc acgacaccct gctcggcgag cggtttgccg aacgtcttcc
     1261 gctccttgac ccgttcgcac atcatctcca gcgaccgctc ggcgacgcct atgagcctca
     1321 tgcagtggtg gatgcggcca ggtcccagac gtccctgggc gatggcgaag cccttccctt
     1381 cgtcccagat gatgttctca atcggcacgc gaacgtcgtt gaaggagatc tcagcgtgcc
     1441 cgtgcggggc gtcgtcgtag ccgaagacgg ggagggttcg ctctatctcc acgcccggcg
     1501 tatcgagggg gaccaggatc atggactgct gctcgtggcg cggcgccgac gtgtccgtct
     1561 tgcccatgaa gatagagatc ttgcacctcg ggtccccagc cccggaggtc caccacttcc
     1621 tgcctttcac cacgtactcg tccccgtctc tctgtatgcg cgccctgatg tttgtcgcat
     1681 cagaagaggc cacgtcgggc tcggtcatgg cgaagcagga ccggatctca ccgtccagca
     1741 gtggcctgag ccaccgctcc ttctgctcct ctgtgccgta gcgtacgagc acctccatgt
     1801 tgcccgtgtc aggggcgttg cagttgaacg cctcgggggc tatggggctg cgtcccatga
     1861 cctcgcaaag tggcgcgtac tcaaggttcg taaggccggc gccgaggtct gagtcaggca
     1921 gaaagaggtt ccatagaccc ctcgtcttcg cctcttgttt gagctcctcc atgatcgggg
     1981 gtaccgacca ccggctctcg gcagtttcta gctgtttgtg gaaagtggcc tcgttcgggt
     2041 agacgagctc gtccatgaac gcctcgatcc gcgtcctgta ttccctcgtc ttgtcggaaa
     2101 actcgaagat cacaacctct cctctcgtaa gagccgcagg accgattctg gcacagtgga
     2161 aggggcgtcg caatcgccgt ggacctgctt ctcccgtaga attagctctc gtaaaggagc
     2221 ggaaggcgat ctgtcaggag gaagttttgg atctcggtct cgaaggcaag gtggcgctgg
     2281 tgacggcggc gagcaagggg ctggggaggg ccgtggctac cgagctcgcc cgagaaggcg
     2341 cgcgggtcgt gatctccagc cgcgacgaag atgctctctc gaagacggcc accgagatac
     2401 acg
//
LOCUS       DATAIR010000773         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_268863, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000773 DATAIR010000000
VERSION     DATAIR010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268863"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VHM69_18335"
     CDS             <1..380
                     /locus_tag="VHM69_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742404.1"
                     /translation="YLKVVGKVLLPLFSESVVHARERRMPMGAKPFRGDRYVRDVSGS
                     PVILGRFRMTGTTDRYLLVVNRSFANRARTRLTMGGAVSRVSKLNISTGAFDKVRLQG
                     RPRRDLVSTIQPGGARLYLLQNA"
     gene            complement(386..751)
                     /locus_tag="VHM69_18340"
     CDS             complement(386..751)
                     /locus_tag="VHM69_18340"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2742405.1"
                     /translation="MPVFDLRALAGEDFCYLTTTGRVSGEPHEIEIWFALVPETRTMY
                     MLSGGGDRSDWVKNLRRNPEVTVRIVEKRFSGFAREAEDVEEDGLARRLLVEKYESRP
                     GSLENWRRTALPLAVDLKM"
     gene            complement(854..2455)
                     /locus_tag="VHM69_18345"
     CDS             complement(854..2455)
                     /locus_tag="VHM69_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2742406.1"
                     /translation="MANIGDYEQTYAGFDWETPERFNFGRDVVDHWSAQDRPAMIWLG
                     TSGEERRLDFAQFSVLSNRFANAAKEMGISRGDRVMVLFGKVPEWHAVLTGLLKLGAI
                     AIPCAPQLRAGDLKFRAEHSGSVAMISGPEGIEAVEKVRNDVPGLYYFISLGGEREGW
                     ESYEALMEAASDDFTAEDTAADEGAYILYTSGTTKHPKGVLHTHGYTHAKWMQAKYWL
                     DLQEGDRLWCTSGTGWAKSIWNVFLGPWSQGTEIFFHEGGFDPAERLELIERYHITVL
                     CQAPTEYRLLAKTPELERATLSSIRHAVSAGEPLNPAVIERWKELHDLTIYDGYGQTE
                     NTLLVANYPGLEVRPGSMGKPSPGCDVKIVDMEGNECPPDEPGDIALAERIPALFKEY
                     WEQPDETEAVFRNGSYLTGDRAYRDTDGYLWFVGRSDDVILSAGYRIGPFEVESILIE
                     HPAVVESAVVPVPDEDRGSVVKAYIVVGNDYEPSEGLAKEIQDYCKANTAPYKYPRRI
                     EFIGDLPKTTSGKIRRVELRQRENE"
BASE COUNT          444 a          789 c          767 g          506 t
ORIGIN      
        1 cctacctgaa ggttgtcggc aaagtacttc tgccgctctt ctcagagtcc gtggtccacg
       61 cccgtgagag gcggatgccg atgggagcga agcctttcag aggggaccga tacgtgagag
      121 atgtcagcgg gagcccggtc atcctcggca ggttccggat gacagggacc acagacaggt
      181 acctgctggt cgtgaaccgc tcgttcgcca acagggccag aaccaggttg actatgggcg
      241 gcgcggtgag cagggtctcg aagctgaaca tatctaccgg cgcgttcgac aaggtccggc
      301 ttcaaggcag gccgcggcgg gacctcgttt cgaccatcca gccgggagga gccagactct
      361 accttctgca gaacgcctga tccggctaca tcttcaggtc caccgccagg ggcaacgcgg
      421 tgcgccgcca gttctccaga ctccccggac ggctctcata cttctcgacc aggagccgcc
      481 gcgcgagccc gtcttcctca acgtcttcgg cctcacgggc aaatccgctg aaacgcttct
      541 cgacaatgcg gaccgttacc tcggggtttc gacgcaggtt cttcacccag tccgagcggt
      601 cgccgcctcc cgagagcatg tacatggtgc gcgtctcggg tacgagggcg aaccatatct
      661 cgatctcatg cggttcgcca ctcacgcgcc cggtcgtcgt caagtagcag aagtcttcgc
      721 ccgcgagagc gcgaagatca aatacaggca tcaggtttca gcagtcagga ctcgcggcgt
      781 gacagaagtt cgagtgcgag cccaggatgt gctttccgca aacctgaagc ctgtagcctt
      841 tattctgaag cctttattca ttctcgcgct ggcgcagttc gacgcgccgg atcttgccgc
      901 tcgtcgtctt gggtaggtcg ccgatgaact ctatgcggcg agggtacttg tacggggctg
      961 tgttggcctt gcagtagtcc tggatctcct tcgcaagtcc ctccgacggc tcgtagtcgt
     1021 tgccgacgac gatgtacgct tttaccacgc ttcccctgtc ttcgtcaggc acgggcacga
     1081 cggcgctctc gacgaccgcg gggtgctcga taaggatgct ctcgacttcg aaaggcccga
     1141 tgcggtaacc ggcagagagt atgacgtcgt cagagcgtcc cacgaaccag aggtagccgt
     1201 cagtgtcgcg gtacgccctg tcgccggtaa ggtaggagcc gttcctgaag acggcttccg
     1261 tctcgtcggg ttgctcccag tactctttga acagcgcggg gatgcgctcc gcgagcgcta
     1321 tgtcgccggg ctcgtccggc gggcactcgt tgccttccat gtccacgatc ttcacgtcgc
     1381 agccgggaga aggcttaccc atggagccgg gccgcacttc gaggccggga tagttggcga
     1441 cgagcagggt gttctccgtt tgcccgtagc cgtcgtagat ggtcaggtcg tgaagctcct
     1501 tccaccgctc gataacagcc gggttgagcg gctcgcccgc ggaaactgcg tggcggatgg
     1561 aggagagggt ggcgcgctca agttcggggg tcttggcgag gaggcggtac tcggttgggg
     1621 cctggcagag gacggttatg tggtagcgct ctatcagttc gaggcgttcc gcggggtcga
     1681 agcctccctc gtggaagaag atctccgttc cctggctcca gggtcccaga aatacgttcc
     1741 agatgctctt ggcccaacca gtccccgagg tgcaccagag cctgtctcct tcctggagat
     1801 ccaaccaata cttcgcctgc atccacttgg cgtgcgtgta gccgtgggtg tgcaggacgc
     1861 ctttggggtg cttggtcgtc ccggacgtat acaggatata tgcgccctcg tccgccgccg
     1921 tatcctccgc cgtgaagtca tccgaggccg cttccatcaa cgcctcgtag gattcccagc
     1981 cttcgcgctc cccgcccagg gagatgaagt aataaagacc tgggacgtca ttacgcacct
     2041 tctcgacagc ttcgatgccc tcgggaccgg agatcatggc cacagatccc gagtgctccg
     2101 ccctgaactt caggtcgccg gcccgaagct gcggcgcgca tgggatggcg atggccccga
     2161 gctttaaaag acccgtgagg acggcgtgcc actcaggcac tttgccgaag aggaccatca
     2221 ccctgtcgcc gcgactgatg cccatctcct tcgccgcgtt ggcgaacctg ttcgagagga
     2281 cagagaactg ggcgaagtcg aggcgcctct cttcgccgct cgtcccgagc cagatcatgg
     2341 ccggacggtc ctgcgcggac cagtggtcta ccacgtcacg cccgaagttg aaccgctccg
     2401 gcgtctccca gtcgaagccg gcgtaggtct gctcgtagtc acctatgttg gccacggtca
     2461 atcacccttc cccaaaggta tctttccaga cccgcatttt actaga
//
LOCUS       DATAIR010000774         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_362160, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000774 DATAIR010000000
VERSION     DATAIR010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362160"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VHM69_18350"
     CDS             <1..1083
                     /locus_tag="VHM69_18350"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX2742407.1"
                     /translation="MVFSAASGPLVLVERVGVDLGSTAPVVYATLASGATLLGVGHIA
                     HASLLRVKYRQRFGETWREDLEPYLIPDALSVLTAGLGVLALLAYDPLAALVVVAGSI
                     ASQVLVYRSREQVKENEDLRARVGSLEEALSTSNTTFGTMIIQDLGQRDGYTHRHAAA
                     TARYAADLAREMKLDETHADRLRMAGLLHNIGLFGLPEELLVTTGRLNSIARSHLDEH
                     PARGAEALAAVSEFKEMANWVRWHHERPDGRGYPDKLRGPWIPLEAKILSVAQAYAAM
                     VLDQPRRPGMEPAEARENLSAGIDTEFDGVVVRALMRLLDTESVGYRMADDHRFVFPS
                     PENGRGVMPGLQDPRHDENLGRSLPQ"
     gene            1116..3170
                     /locus_tag="VHM69_18355"
     CDS             1116..3170
                     /locus_tag="VHM69_18355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2742408.1"
                     /translation="MSRITMASTKAESYRAGLLRISMAGIVALLLSREWPLLDTEGAY
                     LAVGLALLFGVSLIAGRPAILVRERLVGVVLDAVLIGVLIAYTGGGDSPFFPLYLLAA
                     LGIAWIETRRKVVGAAVALVVGYLVAVVVSGGLGALLSTPVALRAASIALFCVVVAFL
                     GAEMNGFRRLAIRLSSTLANEIDRVESDERIISKFGPVLKVLSLEGILGWTVEAAHAV
                     GGGHYAHVAALNGNHHSTLMEGDGDVCPNWWHPSIQSLVLRSCREGEVVRSEEEIHDI
                     EGFMAVPIGTQRDEQWGAIIVGGGEYGVEQERALKLLAEGIASALEAAGEAPGGLDQL
                     TGLPNRASLHRVLRRELTYGRVYTVLAMRVEGFGDYERAYGLDAGDDLLRRIGQRLKE
                     RRQRTFRYGDGEFIVLLGGSDEDRARKVAYALQQHVMEETGGPDHLPLNAAVGFAFTQ
                     KGDEVSVLDAALRALEEARNRPEGIAGLPAGRTFEGLEGDERVAGIVRTLMEALEAKD
                     PYIGEHLRAVSSLAMRIGNELSLAREQVEALTTGAMLHDIGKIGVPDLILQKSGRLTE
                     EEYEVIQRHPVLGVEILTPVRELAPALPVVKHHHERFDGNGYPDGLHGEDIPLLARIV
                     SVADAFDSMIRPRPYGYGISRKAALQEIEDNSGSQFDPVIVRALLEVVEAPGERQANS
                     VG"
     gene            3434..>4125
                     /locus_tag="VHM69_18360"
     CDS             3434..>4125
                     /locus_tag="VHM69_18360"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2742409.1"
                     /translation="MQGLSLDLPTGYYLERDPDVLILRRLDGTMIGAFSARGAAPEAV
                     KRTVEESDRPALQVRFFGHFEMFCDSESMPLGRNGKALTILKYLLANRTRPVSQDHLM
                     GWLWPESNLKKARWSLNSAIHGLRKLLSGCHSSVTTNYVSLEDGYYRLSPSVRVSTDV
                     DEFDERHEKGLQLEKERRMMEAACEYERAIEIYRGDYLVEDLYEDWTMVERERLTNAY
                     MDMLDRLAVNYL"
BASE COUNT          829 a         1150 c         1361 g          785 t
ORIGIN      
        1 atggtgttct ccgcggcgtc cgggcctcta gttctagtgg agagggtggg ggtggattta
       61 ggatcgaccg cccccgttgt ctacgcaacc ctggcatcgg gagcgacatt actaggagtc
      121 ggtcacatag ctcatgcctc actattgagg gtaaaatacc gtcagagatt cggagaaacc
      181 tggcgggagg atctagagcc ttatctgatc ccggacgcgt tgagcgtatt gactgcgggg
      241 cttggggtcc tggctctgct cgcgtatgat cctctcgcag cgctagttgt ggtagcgggc
      301 tccatcgcga gccaggtgct agtgtatcgc tcgcgggagc aggtgaagga gaacgaggac
      361 ctgcgggcgc gagtaggttc gctcgaagaa gccctctcaa cgtccaacac cacgttcggg
      421 accatgatca tccaggatct cggccagagg gacgggtata cgcacaggca cgctgccgcc
      481 actgccaggt atgctgctga ccttgcccgc gagatgaagc tggacgagac ccacgccgac
      541 aggttgcgga tggccggtct gttgcacaac atcgggttgt tcgggttgcc tgaggagttg
      601 ctggtgacga caggcaggct caactccatc gccaggagcc atctcgacga gcatcccgca
      661 cgcggcgcgg aggcgctggc cgccgtctcg gagttcaagg agatggcgaa ttgggtccgc
      721 tggcaccacg agcgccctga cgggagaggc tacccggaca agctgcgtgg tccctggata
      781 ccgcttgagg cgaagatact ctccgtggcg caggcctacg ccgccatggt gctcgaccag
      841 cccaggcgcc ccggcatgga gcccgcggag gcgcgggaga acttgagcgc gggcatagac
      901 acagagttcg acggggtcgt cgtgcgggca ctaatgcgtc tacttgacac cgagtcggtg
      961 gggtaccgta tggccgacga ccacaggttc gttttcccct cgccggagaa cggcagaggt
     1021 gttatgccgg gcttgcagga cccgcgccac gatgagaacc tcggacgaag tctcccccaa
     1081 tagttgtcgc tgaaggaggg aggtgaggta gatagatgag tcggatcacc atggcatcga
     1141 ctaaagcgga atcgtacaga gccgggctgc ttcgcatctc tatggcgggg atcgtagcct
     1201 tgttgttgag ccgggaatgg cctttgctcg ataccgaagg tgcttacctg gcggtgggac
     1261 tcgcgctgct cttcggggtg tccttgatcg ccggccggcc ggctatcctt gtgcgcgaga
     1321 ggctcgtagg agtcgttctg gacgccgttc ttatcggggt gctgatcgct tacaccggag
     1381 gaggggactc gcccttcttc cctctctacc ttcttgcggc gctcgggata gcctggatcg
     1441 agacccgccg gaaggtagtc ggggcggcgg ttgctcttgt ggttgggtat ctggtcgcgg
     1501 tggtcgtctc tggaggtctg ggtgcgctcc tttctactcc ggtggccttg agggccgcat
     1561 ccatcgcgct cttctgcgtg gtcgtcgcat tcctgggtgc cgagatgaac ggtttcagga
     1621 ggctcgctat caggctctct tcgaccctcg caaacgagat agaccgggtg gagagcgacg
     1681 agagaatcat ctcgaagttc ggacccgtac tcaaagtctt gagcctggaa ggcatactcg
     1741 gatggaccgt cgaggcggcc catgcggtgg gcgggggaca ctacgcgcac gttgccgctc
     1801 tgaacggtaa ccatcacagt accctcatgg agggcgatgg cgacgtctgc cccaactggt
     1861 ggcatccctc gatccagagt ctcgtgttgc ggagttgccg cgaaggcgag gtcgtgcgga
     1921 gcgaagagga gatccacgac atagaaggtt tcatggccgt acccataggg acacagagag
     1981 acgagcaatg gggggctatt atcgtcgggg gtggggagta cggcgtcgag caggagcgtg
     2041 ccctgaagct cctcgccgag gggatagcct ccgccctgga ggccgcaggg gaagcccctg
     2101 gcgggcttga ccagctcacg ggcttgccga accgcgcgtc tctccaccgc gttctgcggc
     2161 gggaactcac ctacggtagg gtgtacacgg tcctggcgat gcgcgtcgaa ggcttcgggg
     2221 actacgagcg ggcatacggc ttagacgcgg gtgacgacct cctgcggcgg atcggtcaga
     2281 ggctcaagga aaggaggcag cgcacgttcc gctacgggga tggcgagttc atcgtcctcc
     2341 ttggcggttc cgatgaggat agggcccgaa aggtagcgta cgcgctccaa cagcacgtca
     2401 tggaagaaac aggtggtccc gaccatctgc cgttgaacgc ggccgtaggc ttcgcgttca
     2461 cccagaaggg ggacgaagtg tcagttctcg acgcggcatt gcgcgctctg gaggaggcca
     2521 ggaaccggcc tgaagggatc gcaggattac cggccgggcg caccttcgaa ggattagaag
     2581 gcgacgagag ggttgctggt atcgtcagga ccctcatgga ggccctggag gcaaaagacc
     2641 cctacatcgg ggagcatctg agggccgtgt ccagcctcgc tatgcgcata ggtaacgagc
     2701 tgtccctggc tcgtgaacag gtcgaggccc tgaccacagg ggctatgctg cacgacatcg
     2761 gtaagatcgg ggtgcctgat ctgatcctgc aaaaatccgg ccgcttgacc gaggaggagt
     2821 acgaggtaat acagcggcat ccggtattgg gggtcgagat cctaacccct gtaagagagc
     2881 tggcaccggc cttgcccgtc gtcaaacacc atcacgagcg ctttgacggc aatggatacc
     2941 cggacggtct gcacggcgag gacatcccct tgctggcacg aatagtctcg gtagccgacg
     3001 ctttcgactc catgattcgc ccaagaccct acggttacgg catctccagg aaagccgctc
     3061 tgcaagagat cgaggacaac tcaggcagcc agttcgaccc ggtgatcgtg agggcgctgt
     3121 tggaagtggt agaggctccc ggtgagcgcc aggctaattc cgtcggttag aggcggttgt
     3181 ggctccggtg cgccgctacc aaaaaaatat gcaaatgtac gaagaatcac tcatttgcgt
     3241 gatacggcca cccccgtata cgtaataagc tgaaccagct aagtggtgtc gggcgagatt
     3301 cagacgctga agttctcgtc acaggggaaa ggagcaccag cgatcggttt acgcaacgtc
     3361 tgaaggtaac ttaggcaagc aaggtaagtc gaacctaggc cttccggccc agaatcagcg
     3421 gtctggagaa aagatgcagg gactatcgtt agatctgccg accggctact acctggaaag
     3481 agacccggat gtattgatct tacgccggct cgacgggacc atgatcggcg cgttttccgc
     3541 ccgcggtgcc gccccggaag ctgtcaaacg tacggtcgag gagagcgacc ggcccgctct
     3601 acaggtgcgc ttcttcggcc acttcgagat gttctgcgat agtgaaagta tgccgcttgg
     3661 tcgtaacggc aaagcactga caatactcaa gtacctcctc gccaaccgca cccgccccgt
     3721 atctcaggat catctgatgg gctggctgtg gcctgagtca aacctcaaga aggcccgctg
     3781 gtccctcaac tcagccatcc acggccttag aaagctcttg agcggatgcc attcttctgt
     3841 caccacgaac tacgtctccc tggaggatgg atactacagg ctctctccga gcgtcagggt
     3901 gagcacagac gtggacgagt tcgacgagcg tcacgagaaa ggtctgcagc tggagaagga
     3961 acggcggatg atggaggcgg cctgcgagta cgagagggcg atcgagatct acaggggaga
     4021 ctatctggta gaggacctct acgaagactg gacgatggta gagcgagagc gtctcaccaa
     4081 cgcctacatg gatatgctgg accggctcgc cgtcaattac ctgga
//
LOCUS       DATAIR010000775         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_224761, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000775 DATAIR010000000
VERSION     DATAIR010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224761"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VHM69_18365"
     CDS             complement(<1..1384)
                     /locus_tag="VHM69_18365"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX2742410.1"
                     /translation="MRKVTLSDRLRYRFDNTISKGTVALIGWLFLVLLALVLASTLFV
                     YLTGIAPDTGGHQPGFFETLWLDLMRTIDPGNIGGDQGSWLYLLSMLTVTAGGILVFS
                     TLIGVIFAGIDEKLADLRKGRSFVVEQGHTVIYGWSPEVFPVVAELVKANQSRQGACI
                     AVLAENDKVEMEDEIRARVGKTGNTRVVCRTGDPINIEDVEIVNPDEARSIIVLPPEG
                     DEADSRVIKTILALTNNPNRREEPYHIVSRIHDPENLGVARMVGRDELELVAVDDFIA
                     RITAQTCRQSGLSVVYTDLLDFGGDEIYIPREHSLAGKTFGEALTAYEKCSVIGLRKA
                     EGRIELNPPPDTPISEDDKLVLIAEDDSTIEGFATQKQEIDADSIRDPRSPEPKPERN
                     LILGWNDRAPTIVSELDHYVHPGSEVTVVTPEDAVPHFDDLRNLTVAPRDGNITDRHV
                     LNDLGVASYDH"
     gene            1587..2036
                     /locus_tag="VHM69_18370"
     CDS             1587..2036
                     /locus_tag="VHM69_18370"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2742411.1"
                     /translation="MAQGVSGAPAALEVHVTGRRVLATIVDGIVLTAGFWVMSALFGT
                     TSVEGGQVGASLSGLAALGSFILVFAYYILLEGFLGQTLGKMLLGIKVVKEDTGEVPG
                     LGAATLRTLLRIIDSLFFYLVAFISVLASQQNKRLGDMVAHTLVVRK"
     gene            complement(2106..>2592)
                     /locus_tag="VHM69_18375"
     CDS             complement(2106..>2592)
                     /locus_tag="VHM69_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2742412.1"
                     /translation="LDGFSKTYAMTGWRLGYGVMPEPLAEEVARLQVNSNSCTSAATQ
                     YAGLEALHGPQDAVQSMLAEFRARRDLVVSGLNELPGVECITPQGAFYAFPRISGTGL
                     SADALADLLLDEAGVACLSGTAFGRFGEGHLRLSYANSRENISRAMESMGEVLSRVAP
                     G"
BASE COUNT          453 a          771 c          839 g          529 t
ORIGIN      
        1 catggtcgta ggaagcaacc ccgaggtcgt tcagaacgtg gcgatcagta atgttgccgt
       61 cacggggagc gacggtcagg ttgcggagat cgtcgaagtg gggaacggca tcttcggggg
      121 tcaccacggt cacctccgag cccggatgca cgtagtggtc gagttcggag acgatcgtgg
      181 gggcgcgatc gttccagccg aggatcaggt tgcgctcggg tttcggctct ggggaccggg
      241 gatctcgtat agaatcggcg tcgatctcct gtttctgcgt tgcgaatccc tcgatcgtgc
      301 tgtcgtcttc ggcgatgagc acgagtttgt cgtcctctga gataggggtg tccggaggcg
      361 ggttcaactc gatacggcct tccgctttgc ggagtccgat caccgaacac ttctcatagg
      421 ctgtgagggc ttcgccgaac gtctttccgg cgaggctgtg ctcgcgaggg atatagatct
      481 catctccccc gaaatcgagc aggtctgtgt agacgacgga gaggcccgac tggcggcagg
      541 tctgggccgt gatgcgcgcg atgaagtcgt ccaccgccac cagctccaac tcgtcgcgtc
      601 cgaccatccg cgccaccccg aggttctccg gatcatggat tctcgacacg atgtggtatg
      661 gctcctcgcg ccggttcggg ttgttggtga gggcgaggat agttttgatc acgcgcgagt
      721 cggcctcgtc tccctcaggt ggaagcacga tgatcgagcg cgcctcgtcg gggttgacga
      781 tctcgacgtc ttcgatgttg atggggtctc ctgtcctgca aacgacgcgc gtgttgccgg
      841 tcttgcctac cctggcccgg atctcatcct ccatctcgac cttgtcgttc tcggccagta
      901 cggctatgca cgcaccctgg cggctctggt tggccttgac cagctcggcc acgacgggga
      961 agacctcggg agaccagccg tagatgaccg tgtgcccctg ctcgacgacg aatgagcgac
     1021 ccttccgcag gtccgccagt ttttcgtcta taccggcgaa gatgaccccg atcagggtgc
     1081 tgaagacaag gatcccgccc gccgtgacgg tcagcatcga aaggaggtaa agccagcttc
     1141 cttgatctcc gccaatattg ccgggatcta ttgtccgcat caggtcgagc cacagcgtct
     1201 cgaagaagcc cggttggtgc ccgcctgtgt caggcgctat cccggtcagg tagacgaaca
     1261 gggtactcgc caggaccaac gccagaagca ccaggaagag ccagccgatc agtgcgactg
     1321 tgccttttga gatggtattg tcgaaccggt atcgcaaccg gtcggagagc gtgacctttc
     1381 gcatacccct ccgcgccagc agccgacgaa tggatgagtg atgcattata tcgctccatg
     1441 cgcgcctgta gtcggtcggg agcatcccgc ctctagtaca ttcgcggtag cgaaaattaa
     1501 ctcatttggg ggatgtgcat gaccgcgggc cagatcatca ttatgccgtc tatgtaaggg
     1561 cttgacccga aggaaggaga tacattatgg cccaaggagt atcgggagcg cctgccgcgc
     1621 tggaggtcca cgtgacgggc aggagggtac tggcgacgat agtggacggc atagtcctga
     1681 ccgcggggtt ctgggtgatg tccgcgctct tcggcacgac ctcggtagag ggtgggcagg
     1741 tcggcgcttc tctgagcggc ctcgccgcgc taggctcctt catcctggtc ttcgcctact
     1801 acatcttgct agagggcttc ctgggacaga ccctgggcaa gatgctcctc ggcataaagg
     1861 tcgtgaagga ggatacgggc gaggtccccg gcctgggtgc tgccacctta aggaccctgc
     1921 tgcgcatcat cgactccctg ttcttctacc tggtggcctt catctccgtt ctcgcctctc
     1981 agcagaacaa gcgcctcggt gacatggtcg cccacacgct ggtagttcgt aagtagatac
     2041 gtagggtcgg gccgggactg ggggaggagt cccggcccgc acaaggtctg aggactttgt
     2101 gcggcttatc ctggcgccac ccgagacagg acctcgccca tactttccat ggccctgctg
     2161 atgttctcgc gcgagttggc ataggagagg cgcaggtggc cctccccgaa acgtccgaac
     2221 gccgttcctg agaggcaggc gacacccgcc tcgtcgagga gtagatctgc cagtgcatcc
     2281 gcgctgaggc cggttccgct gatacgcggg aaggcgtaga acgcgccctg gggcgtaatg
     2341 cactcgacgc cagggagctc gttgaggccg ctgacgacga ggtccctgcg cgccctgaac
     2401 tcggcgagca tgctctgcac ggcgtcttgc ggaccgtgga gggcctcgag gccggcgtac
     2461 tgggtggccg cgctggtgca cgagttggag ttgacctgta gcctggctac ctcttccgca
     2521 agaggctcgg gcatcacgcc gtagccgagc cgccagcccg tcatcgcgta cgtcttcgag
     2581 aagccgtcga gg
//
LOCUS       DATAIR010000776         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_224934, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000776 DATAIR010000000
VERSION     DATAIR010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224934"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VHM69_18380"
     CDS             <1..684
                     /locus_tag="VHM69_18380"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2742413.1"
                     /translation="LSRGVGDPEDTFGYAGNGRFMAVINRGDYWQIAYVIRKGEYESV
                     RAEGLEAFQRSIGGAIPEFSNRTSELRDWADVKLLTVQADRLRRWFRPGLLCIGDAAH
                     AMSPVGGVGINLAIADAVAAANALAGPLGAGRVRTRDLMVVQLRRELPTRVIQALQSF
                     AQRRVISSSLQQGGAPSLPGFVSAILRPARDLPARIIAFGIWPEHVRRREQAVRTGTT
                     PLAGKESVK"
     gene            681..2537
                     /locus_tag="VHM69_18385"
     CDS             681..2537
                     /locus_tag="VHM69_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742414.1"
                     /translation="MNAFRVLATPRVWGPILGVVTVLALIFYAYLAAVASPQENLEDL
                     PVALVNEDRGGDLGGKEINLGNAVAEKVTDPDSPAAGTVEWMRPDTREEALKGIGNNE
                     YYGAIVIPKDYTQRISDLVSPPTIPIAVVVEDQGAEINGQPMKLGEEVAKRITASDSP
                     APEFVKWTRIDDRDEAMEGLESGAYYAAIVIPGDYSQSLVGMSGPPAGVPPSGQPPAL
                     EPARIDLLTSPVVRPSTTSLIENAFSGMVGGISGATSERMLGVLSEQGAPVPPGAGAM
                     ISDPVKGKISEADVSSEAGPLPKAPEPAEIEVLTNPSAGQAVSAPVQNISTGIVDAVS
                     AATSERIAEQAGEHGAQILPEVAAVVGDPVRADVTEAQPVGPDSGNGQSPFFLSFLAN
                     LSGLVGGAVIFFLIRDAAERLEARGTSPSRTGLWTVRLLLGLVYAALVAGAELWVAFG
                     LLGVEHEASTMQVYLFLSLAIAAIVSITMLFAVLFGPAGIGISAVLNVILGLVSSGGA
                     APLEALPPFYQAYADWLPIRYAIDGLRSLLFYDGSLDAASLEGGWRDSLWFLGSDGRS
                     EAAGLEDAVWMIGAYLAGAAALGYVISLLRDLATRRGRREITNPPESAEAAG"
     gene            2741..>3197
                     /locus_tag="VHM69_18390"
     CDS             2741..>3197
                     /locus_tag="VHM69_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2742415.1"
                     /translation="MSETKQRKIYDPTSLERRWQERWAESGLYKMDWDSDKPKHYALT
                     MLPYPSGDLHVGHWYAMTPSDTRARFMRMRGYNVFFPIGFDAFGLPAENAAINRGIHP
                     YKWTMSNIENMRGQLRQMGTMFDFDAEVVTCDPDYYRWNQWFFVRFFEKG"
BASE COUNT          598 a          992 c         1033 g          574 t
ORIGIN      
        1 ctctcgcgtg gagtgggaga ccccgaggac accttcggct acgctggcaa cgggcgcttc
       61 atggccgtca taaaccgcgg cgactactgg cagatcgcct acgtcatcag gaaaggcgag
      121 tacgagagcg ttcgtgccga aggtctcgaa gcgttccagc gctccatagg aggggcgata
      181 cccgagttct cgaaccgtac gagcgagctg cgggactggg ctgacgtgaa gctgctgacg
      241 gtgcaggccg acagactgcg ccggtggttc aggccaggac tgctgtgcat cggtgacgcg
      301 gcccacgcga tgtcgccggt gggtggcgtg ggcatcaacc tcgccatagc ggatgcggta
      361 gcggcggcga acgcgctcgc cgggccgctc ggggcagggc gggtgagaac acgtgacctg
      421 atggtcgtgc aactcaggcg ggaactgccg acgcgcgtta tacaggcact acaatccttc
      481 gcccagcggc gtgtgatctc ctcgtccctg caacagggtg gagcgccgtc tctccctgga
      541 ttcgtcagtg cgattctgcg ccccgcgcgc gaccttccgg cccgcatcat cgccttcggc
      601 atctggccgg aacacgtcag gcgcagagag caagcagtca ggaccggcac tacgccgtta
      661 gcaggaaagg aatcggtgaa atgaatgcgt tcagagtact tgcgaccccg agggtctggg
      721 gaccaatcct tggcgtggtg acggtacttg ccctgatctt ctacgcttac ctggccgccg
      781 tagcaagccc acaggagaac ctcgaggacc tgccggtggc ccttgtgaac gaggacagag
      841 gcggagacct cggaggaaaa gagatcaacc tcggtaacgc ggtcgccgag aaagtcactg
      901 acccggactc accggcggcc ggcaccgtcg agtggatgcg tcctgacacc cgcgaggagg
      961 cactgaaggg catcggcaac aacgagtact acggcgccat cgtgatcccc aaggattaca
     1021 cacagcggat ctccgacctc gtgagcccac cgaccatacc tatcgcagta gtggtcgagg
     1081 accagggtgc tgagataaac ggccagccga tgaagctcgg cgaagaggtg gcgaagagga
     1141 tcaccgcctc cgactctccg gctccggagt tcgtaaagtg gacccgtatt gatgaccgcg
     1201 acgaggccat ggaaggtctc gaaagcggcg cgtactacgc tgccatcgtt atccccggtg
     1261 actactcgca gagtcttgtc ggcatgtctg gcccaccggc tggtgtgcct ccttccgggc
     1321 agccaccggc tctcgaaccg gcaaggatcg acttgctgac gagtccggtg gtgcggcctt
     1381 ctaccacgtc cctgatagag aacgccttca gcggcatggt gggggggatc tccggggcca
     1441 cgagcgaacg catgctgggg gttctttccg agcagggtgc gcctgtgccg ccgggtgcag
     1501 gagcgatgat ctccgacccc gtgaagggga aaatctccga ggccgacgtc tccagcgagg
     1561 ccggaccgct tccgaaggcc cctgagcctg ctgagattga ggtgctcacg aacccttcgg
     1621 ctggacaggc tgtctcagct cccgtacaga acatctctac gggtatcgtt gatgcagttt
     1681 cggcggcaac gagcgagcgc atcgcggagc aagccggaga gcacggggcg cagatactgc
     1741 cagaagtcgc cgctgttgtc ggggacccgg tacgggccga tgttacggag gcgcagcctg
     1801 tcggacccga ctccggcaac gggcagtcgc cattcttcct ctcgttcctg gcgaacctct
     1861 cgggtctggt cgggggggcg gtcatattct tcctgatccg cgatgccgcg gagcggctgg
     1921 aggcgcgggg cacgtctccg tcacgcaccg ggttgtggac ggtgcgactt ctcctggggc
     1981 tcgtatacgc cgcgctcgtg gcaggagccg agctgtgggt cgccttcggc cttctcggtg
     2041 tcgaacacga ggcgagcacg atgcaggttt acctgttcct ctcgctcgcc atcgcggcga
     2101 tcgtgtcgat cacgatgctc ttcgcggtac tatttgggcc tgcggggatc gggatcagcg
     2161 ctgtgctcaa cgtgatcctt ggtctcgtct cctccggcgg cgcggcgccc ctggaggcgt
     2221 taccaccctt ctaccaggcg tacgccgact ggctgcccat tcgctacgcc atcgacggcc
     2281 tgcgctcact gctcttctac gacggctccc tcgacgcagc gagtctcgaa ggtggttgga
     2341 gggattccct gtggttcctc ggtagtgacg gacgctcaga agcagctggt ctcgaggacg
     2401 cagtgtggat gatcggcgct taccttgcgg gcgcggcggc tctggggtat gtgatctcac
     2461 tgctccgcga cctcgcaacc cggcgcggac gtagagagat cacgaaccca cccgagagtg
     2521 cagaggcggc gggatagaac gccggcgcac gaccctcccc ggccgcccgg tgtcacaatc
     2581 gggcggccta ctcctttcat aattagtgtc gccgatgata tattaagggc atgaacgcca
     2641 gggatcacaa ggttcagaag tcggactccc gactataaag ccctgcgcgg atttcccctg
     2701 atctagactc accccaagcc cgtaacagaa ggagtctctc gtgagcgaga cgaaacagag
     2761 aaagatttac gatcctacgt ctctcgaacg ccgctggcag gagcggtggg ccgagagcgg
     2821 cctctacaag atggactggg actcggacaa accgaagcac tacgcgctca ccatgcttcc
     2881 gtatccgagc ggcgacctcc acgtgggcca ctggtatgcc atgacgccct ctgacacgcg
     2941 ggcgcgcttc atgcggatgc gcggctacaa cgtgttcttc cccataggct tcgacgcatt
     3001 cgggctccct gcggagaacg ccgccatcaa tcggggcatc cacccgtaca aatggaccat
     3061 gtccaacatc gagaacatgc gcggccagct caggcagatg ggcacgatgt tcgacttcga
     3121 cgccgaggtc gtcacctgcg acccggacta ctaccgctgg aaccagtggt tcttcgtgag
     3181 gttcttcgag aaaggac
//
LOCUS       DATAIR010000777         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_168268, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000777 DATAIR010000000
VERSION     DATAIR010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168268"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHM69_18395"
     CDS             complement(<1..429)
                     /locus_tag="VHM69_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2742416.1"
                     /translation="MKKVCVFDVNETLLDLAALDPHFERAFGDASARKLWFNQMIQSA
                     FVATITDAYATFGEAATAALRMTEERQGVEISDEDRQEILGGLRTLPPHPEVPESLDR
                     LREAGFRLATLTNSTQEVAEAQIVNAGLTDRFEQILSADTA"
     gene            complement(597..1520)
                     /locus_tag="VHM69_18400"
     CDS             complement(597..1520)
                     /locus_tag="VHM69_18400"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX2742417.1"
                     /translation="MEKFIISAAITGGMTVPGQSEAIPITPEEIVDSAVEAHEAGATI
                     VHLHVREPETGKPSSDIGLFREVVSGIKDRCDVIVQPTTGGGKGMTLEERAAVLPELE
                     PEMATFNTGSFNFGLFPIAERGGDFEEWEIDYLEGTRDYIFRNTFADMEKVCGMFREA
                     GAKPELEAYDVGHLYNIKYLLGKGLLDTPIHIQFVLGVMGANAATIEQFMHMRHTATE
                     LFGEDYTWSAAGVGYPAEYHLAAMCLMLGGHVRVGLEDNLRVSRSKRAESNAELVEKA
                     VALGALFDREPATPDEAREAFGLKGRAAVAF"
     gene            complement(1588..2502)
                     /locus_tag="VHM69_18405"
     CDS             complement(1588..2502)
                     /locus_tag="VHM69_18405"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX2742418.1"
                     /translation="MVDHAWEQVLPPLPLEEWEESKETLHRYVQVVGKIRLTYAPFMN
                     HWWHVTLYVSPRGLRTSPIPYGHTTFEISFDLLDNRLVVSTSEGGGFAFALDDLPVAE
                     FYRRLVDGLGTLGVHVAINTTPFDLGDEQTLDENVLNCVCDPEYVGRYGRVLAQVDQI
                     FKEFAGRFNGKQSPAQLFWHGFDLAATRFSGRRAPEREGADAVTREAYSHEVISFGFW
                     PGDINVREPAFYSYTAPEPVGLTDQPLRPASASWAPEGGMALLMYEDVRTSDSPRATL
                     LAFMESAYQAGAKTAGWDIEDFRTGHAG"
     gene            complement(2521..>3897)
                     /locus_tag="VHM69_18410"
     CDS             complement(2521..>3897)
                     /locus_tag="VHM69_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX2742419.1"
                     /translation="DGGKQPLVTPDGNLYLVGNGEVYNHEEIRETLDNDELSTRSDNE
                     VALRLVAERGPEALSELLGMYAFLIAGEDGTFIAARDPVGIKPLYWARRDGLVHFASE
                     RSAFDEDWQPDVASFPPGHYWTPEGGLVRFAPPVPPKDQMEHFEGPGEPGAEIPGDIL
                     EKVRDRLIRTVERQMMGDVPVGVFLSGGLDSSLVAAIAARWYQKRGEKLKTFAVGLED
                     SPDLIAARAVAEYLDTEHHESVYTAEDALSALPKVVRTIESFDPSLVRSAVPNYILAE
                     FTAKHVKVVLTGEGADEIFAGYEYLEDFRKEEDLHAELVRTIEGLHDLNLQRADRVTM
                     AHGLEARVPFLELDMISLGLSLPAGWKLAGEGQDEKRLLRQAFDGWLPDDFLWRKKAQ
                     FGDGSGAASVLKQRMEESVTEEEFERERHEVEPPLRTREEVAYYRIFAEDLGEVRPKR
                     TIGRFATA"
BASE COUNT          637 a         1229 c         1265 g          766 t
ORIGIN      
        1 cgcggtgtct gccgagagga tctgctcgaa cctgtccgtt agccctgcgt tcactatctg
       61 ggcctcggcg acctcctgcg tcgagttggt aagggtagct agacggaaac ctgcctcgcg
      121 tagtctgtcc aggctttcag gcacttcagg gtgcggcggc agggtgcgca gtccgcccag
      181 gatctcctgt ctgtcttcgt ccgagatctc cactccctgc cgctcctcgg tcatcctcag
      241 ggctgctgtt gcggcctcgc cgaaggtcgc gtaagcgtcg gttatcgtgg ctacgaaggc
      301 tgattggatc atctggttga accacagctt tcgcgccgaa gcgtcgccga aggcccgctc
      361 gaaatgaggg tcgagggcgg cgaggtcgag caaagtctcg ttcacgtcga agacgcacac
      421 ttttttcatg cgtggtcctt tctcttagca atgctctcag cacgctacgg cctcacggcc
      481 ttcgctttca gcctgtcagc aacagacaaa aagctgaagt gctgactggc tgacaagccg
      541 ccaaaggcgg cgtgctgacc ggcgcgattt gcagaaggtg ccttctgcaa atcgctttag
      601 aatgctacgg cggcccggcc tttgaggccg aacgcctcac gcgcctcgtc aggggtggcc
      661 ggctccctgt cgaacagggc gccgagggcg acggccttct cgacgagctc cgcgttcgac
      721 tcggcgcgct tgctccggct gacgcggagg ttgtcttcga ggccgactct gacgtggccg
      781 ccgagcatca ggcacatcgc ggcgaggtgg tattcggccg ggtaaccaac gcctgccgcg
      841 gaccacgtgt agtcctcgcc gaagagctcc gtcgcggtgt ggcgcatgtg catgaactgc
      901 tcgatggtgg cggcgttcgc gcccatcacg ccgaggacga actggatgtg gatgggggtg
      961 tcgagcaatc ctttgccgag taggtacttg atgttgtaga ggtgtcccac gtcgtaggct
     1021 tcgagctccg gtttcgcgcc cgcctcgcgg aacattccgc agaccttctc catgtcggcg
     1081 aaggtgttgc ggaagatgta gtcgcgggtg ccttcgaggt agtctatctc ccactcttcg
     1141 aagtctccgc cccgctctgc gatggggaag aggccgaagt tgaagctgcc cgtgttgaag
     1201 gtcgccatct cgggctccag ctccggcagg acggcggccc gctcttcgag cgtcatgccc
     1261 ttcccgccgc ccgtggtggg ctgcacgatc acatcacacc gatccttgat ccccgagact
     1321 acctcgcgga agagcccgat gtccgagcta ggtttgccgg tctccggctc gcggacgtgg
     1381 aggtggacga tggtcgcgcc tgcctcgtgc gcctccactg cggagtccac gatctcctct
     1441 ggcgttattg gtattgcctc gctctgccca ggcaccgtca tgccgcccgt tatggccgcg
     1501 ctgatgatga acttctccac acccgctccc ttcggtagag tcccccctca atactagaga
     1561 cccgcccgga ccgccgcaaa cggtcgctca accggcgtgt cctgtcctga agtcctcgat
     1621 gtcccaacct gcggtcttcg cccctgcctg ataggcgctc tccatgaagg cgaggagggt
     1681 cgctctggga gaatcactcg tgcggacgtc ctcgtacatc agcagcgcca tccctccttc
     1741 gggagcccac gatgcgctcg ctggccgtaa gggctgatct gtcaatccca ctggttcggg
     1801 ggctgtatag gagtagaaag ccggctcccg cacgtttatg tcgcccggcc agaagccgaa
     1861 ggagatgacc tcgtgcgagt acgcttcgcg ggtcacggca tcggcgccct ccctctcagg
     1921 agcgcggcgg cccgagaagc gggtggcggc gaggtcgaag ccgtgccaga agagctgggc
     1981 ggggctctgc ttgccgttga aacggcccgc gaactccttg aagatctggt ctacctgcgc
     2041 aagcacccgc ccgtagcgcc cgacgtactc cgggtcacag acgcagttga ggacgttctc
     2101 gtcaagcgtc tgctcgtcgc ccaaatcgaa gggggtagtg tttatggcga cgtgtacacc
     2161 taaagtgccg aggccatcga cgagcctgcg gtagaactcg gcgacgggca ggtcgtccag
     2221 ggcgaacgcg aaacctcctc cctcgctcgt cgagaccacg agccggttgt caagcaggtc
     2281 gaaggagatc tcgaacgtgg tgtggccgta ggggatcgga gatgtcctca gcccgcgcgg
     2341 gctcacgtac agcgtgacgt gccaccagtg gttcatgaaa ggcgcgtagg tcagccggat
     2401 cttgccaacg acctggacgt accggtgcag ggtctctttg ctttcctccc attcttcgag
     2461 gggaagaggc ggcaatacct gctcccatgc atgatcaacc accgtcctgc tcctggaagc
     2521 tcaggcggtt gcgaagcggc cgatggtgcg cttcgggcgt acttcgccga ggtcttcggc
     2581 gaagatgcgg tagtaggcga cctcttcgcg ggtgcgcaag ggaggctcga cctcgtggcg
     2641 ctcgcgctcg aactcctcct ctgtgacgct ctcctccatg cgttgcttga ggacggaggc
     2701 ggcgccggaa ccgtcgccga actgggcctt cttgcgccag aggaagtcgt ctgggagcca
     2761 gccgtcgaag gcctggcgga ggagccgttt ctcgtcctga ccctcgcccg cgagcttcca
     2821 cccggcgggc agggagaggc cgagtgagat catgtcgagc tccaggaaag gtactcgtgc
     2881 ttcgagtccg tgcgccatcg tgacgcggtc ggccctctgc aggttcaggt cgtgcaaccc
     2941 ttcgatggtg cggacgagct cggcgtgcag atcctcctcc ttgcggaagt cctccaggta
     3001 ctcgtatccg gcgaagatct catcggcgcc ctcgccggtg aggacgacct tgacgtgctt
     3061 cgccgtgaac tcagcgagga tgtagttggg gaccgcgctc cgcacgagcg aagggtcgaa
     3121 gctctcgatg gtacgtacga ctttggggag cgcactgagc gcgtcctccg ccgtgtagac
     3181 gctctcgtga tgctccgtat ccaggtactc ggcgacggcg cgggcggcga tcagatccgg
     3241 cgagtcttcg agcccgacgg cgaaggtctt gagcttctcg ccgcgcttct ggtaccagcg
     3301 ggccgcgatg gctgcgacga ggcttgagtc gagtccgcct gagaggaaga ccccaacagg
     3361 cacgtcgccc atcatctgcc gctcgacggt cctaatgaga cggtcacgga ccttctccag
     3421 tatgtcgccc gggatctcag ctccgggctc gccaggtccc tcgaagtgtt ccatctgatc
     3481 tttgggaggg acgggggggg cgaagcggac gaggccacct tctggggtcc agtagtggcc
     3541 tggtgggaag gacgcgacgt caggctgcca gtcctcgtcg aaggcgctcc tctcagaggc
     3601 gaagtggacg aggccgtcac ggcgcgccca gtaaaggggt tttatgccga cggggtcgcg
     3661 cgctgcgatg aaggtgccgt cctcgccggc gatcaggaac gcgtacatcc cgagcaactc
     3721 cgagagggcc tcagggccgc gttcggcaac gaggcgcagc gcaacctcgt tgtccgaacg
     3781 ggtgctcaac tcgtcgttgt cgagggtctc cctgatctcc tcgtggttgt agacctcgcc
     3841 gttgccgacg aggtagagat tcccgtcagg cgtcacgagc ggctgctttc caccatc
//
LOCUS       DATAIR010000778        11389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_269764, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000778 DATAIR010000000
VERSION     DATAIR010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269764"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VHM69_18415"
     CDS             complement(<1..460)
                     /locus_tag="VHM69_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742420.1"
                     /translation="MLGGDTPFVLLVLNAGFALSLLLVPLSIGVAVLRSRLFDIDVLI
                     NRTLVYGLLTALLVLVYFGGVTVTQAIFQALTGQQELPQLTIVVSTLVIAALFNPLRH
                     RIQSFIDRRFYRRKYDARKTLEEFSTRLREETDLDALSDEMVGVVRETMQP"
     gene            complement(479..1249)
                     /locus_tag="VHM69_18420"
     CDS             complement(479..1249)
                     /locus_tag="VHM69_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742421.1"
                     /translation="MSTRASPPATTLSGRWLPFARLLWAAVAITALAIVLISVPSSLE
                     QYRSVCTAASEVCSERAIEQPTPEGVRALRDVGLSVRSYALLNVLIDKGFQLVWFMLG
                     ALIFWRRSDDRMALLVSAFLVSFGTVTVDTTDADALVSSQPAWWLPVQGVQIMGAVCV
                     VLFFLLFPGGRFVPRWTRWLAVVFIAFEVSRDRFPDLYSRSPVLEMVSLGVFSGMIVS
                     LVWSQIYRYREVSSEAQRRQTRWVVFGTTLGVAASFPF"
     gene            complement(1774..4542)
                     /locus_tag="VHM69_18425"
     CDS             complement(1774..4542)
                     /locus_tag="VHM69_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2742422.1"
                     /translation="MRLHDLGIDQLLESIRDAVVVVDARSGRIVLWNAAATKIFGYSS
                     SEALEHSWTVIVPERLKAQCEAGMARYRDTGHGPYIDSHAVLELPAVRKDGEEIAIEI
                     ALSPIEPIRDSEVRNGRFVLAIVRNITERKEVENAIEERAEHFRTLIQNTSDIITLLE
                     ADGTVRYISPAVERVTGYMPEEQIGTNAFGAVHPDDRERALGIFADVLKRPGLHPPIE
                     FRVPHKDGSWRNVEHIVNNLLDDPAVMGVVVTSRDLTKRRQAEIRAQEADFKYRALVE
                     HIPAIVYIEDVETQATLYDSPQIEAMLGYPADTYEKDPHYWVKILHPEDRRRVMAEEV
                     AATERGEFKLEYRVLASDGRVVWLRDEASIIWDEEDRPRFWQGVIFDITERKRTEEEV
                     RRLNETLEQRVAERTVRLQAALTKLEERERGLRESEQLYRTVVEQAAENIFLVDAETK
                     QILEANAAFYESLGYREQELLRMTLYNIVAHDQESVDDSVRRIMNRGHHFIGERRFRR
                     KDGSLADVEVSVSVIPYRGREAMCVVAHDITERKQTEKALIASERRFRQLFENSADVL
                     FVHDEHGRFVDCNAEACRALGYTREELLLLSVADVATHLISEEERRERKGNTLWERAI
                     QGEPGKIVGFEENELRRKDDTIFPVEVGVGAIEYEGRHLIFASIRDTTERKRAEEELR
                     RFNEELEERVRRRTAQLEAFNSELEAFSYSVSHDLRAPLRAIDGFSQILLEDYKEKLN
                     EEGRDYLRRTKDASQRMGQLIDDLLDLSRVTRGEMGRERVNLSDVARKIVNELGRVQP
                     GRQVEVVIEQGLVANGDGRLLRVVLENLLGNAWKFTRDQTHPRIELGTLEYESTQAYF
                     VRDNGVGFDMAYADKLFGAFQRLHSMGEFEGTGIGLATVQRIIHRHGGRVWAKGEVGK
                     GATFFFTL"
     gene            4918..5481
                     /locus_tag="VHM69_18430"
     CDS             4918..5481
                     /locus_tag="VHM69_18430"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2742423.1"
                     /translation="MGQLITDLFITLDGYASGEGAPAYYGYHGPELERWVDEHLATPQ
                     VLLMGRVTYEVLSAIAQNHPVEGPDRMSELPKVVFSRTLREPLAWDNARVARADLVEE
                     VRRLKAESDAPLRTIGSLSVVKGLLEAGMVDRLRLTVFPLILGSTGREPVFAGLPDIH
                     LELVDTEVLDARLVTLEYHPATTQQTD"
     gene            complement(5855..7225)
                     /locus_tag="VHM69_18435"
     CDS             complement(5855..7225)
                     /locus_tag="VHM69_18435"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX2742424.1"
                     /translation="MPDPGHRPSLEEQIDQWRSYLRRRQAIHSADVEELEDHLREQIA
                     VLVDAGLATDEAFLVAVKRMGKLDALSREFAREHSERLWKQLVVVPSGSGEPRARART
                     DAIVVFCLAVAAALLIKVPALFGIELDQDAGFYARNLSLFVLPLLTGYFVWKRRLGTS
                     TLLWLAVAFVAAGVFANVYPFAPEGYTEVLTALHLPIALWLVVGIAYAGGRWGQVGGR
                     MDFIRFSGELFIYYVLIALGGGVLMAFMFMIFQSIGIDVEPFFESWLLPCGAVGAVMV
                     ASWLVEAKQSVIENMAPVLTRLFTPLFAAVLVTFLGTLLWTGRGVGIERSVLIAFDLL
                     LVLVLGLLLYSVSARDPRSPPGAFDVVQVVLVVSALLADAVALWAIAARITEFGFSPN
                     RVAALGENVILLVNLAWSIVLYIRFLRGRRSFTGLERWQTDYLPVYAVWAAIVVIVFP
                     PLFGYI"
     gene            complement(7228..7629)
                     /locus_tag="VHM69_18440"
     CDS             complement(7228..7629)
                     /locus_tag="VHM69_18440"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2742425.1"
                     /translation="MTREIMEINKDLIAASSTPIVLAILAEDDSYGYAILQRVRELSG
                     GRMEWTDGMLYPVLHRLERLGHVEARWEVAGSGRRRKYYRITPGGRDQLAEERRQWQA
                     VDNTLRGIWQAHSHPVPASLPASAAPLPQGV"
     gene            complement(7868..8101)
                     /locus_tag="VHM69_18445"
     CDS             complement(7868..8101)
                     /locus_tag="VHM69_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742426.1"
                     /translation="MGEAIGAVMAAADSMVFGRVTYEAILDALWQFSKRMSYSFGEGI
                     RLQVFLLMPETFTHLRFTLSANRSSSHSAVHKY"
     gene            complement(8224..9663)
                     /locus_tag="VHM69_18450"
     CDS             complement(8224..9663)
                     /locus_tag="VHM69_18450"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2742427.1"
                     /translation="MADNRQDALGSHRDRLLDEVAIRAFSSRVRGQLIRPDDDAYDVA
                     RAVFYGGIDRHPALIVRAADATDVSRVVALSRESGLELAVRSGGHSPAGHSTTEGGIL
                     LDLSQMKGLEIDLERRTAWAETGLTAGEYTAAAGAHGLATGFGDTGSVGIGGITLGGG
                     VGFLVRKHGLTIDDLLAADVVTADGQLLRVDAERHPDLFWAIRGGGGNFGVATRFEYR
                     LHEVDTIVGGMLILPATPEVVASFVAEAEAAPEELSAIANVMTAPPMPFVPAEMHGQL
                     IIMALLVYAGEVEAGERAIAPFRGLAEPIADMVKPMPYPEIYPPEEDGYHPIGSGRTM
                     FLETIDGAVAQTIVDHLKNSSAQMTVAQIRVLGGAMARVPAEATAFAHRGSRIMVNVA
                     ALYEHPDEAAVYEPWVTDFAAALHRGDEVGAYVGFLGDEGEGRVREAYPGKTLEMLTA
                     IKGRYDPTNFFRLNQNIRPEAQPTSEAVS"
     gene            9929..>11389
                     /locus_tag="VHM69_18455"
     CDS             9929..>11389
                     /locus_tag="VHM69_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742428.1"
                     /translation="MDRQILRAKLYVPRARLDNVARPRLYERLDEGVRRELTVVSAPA
                     GFGKTTLLADWSRRGELPVAWVSLDERDDDPVRFLLYLIAAIGTIHQGFGETTRAFLS
                     SLLSQEELEPVLTALSNEILELPSDFVLVLDDYHAIRSEAVHDALAFLLDHSPPPMHL
                     VLAGRTSPPLPLPRLRARGRLTELGVSDLRFTLEEASDFLGRTMDLSLTAERVATLER
                     GTEGWIVGLQLAAHALRGGDARPVEAFTGSARHVVDYLADEVLSRQPEEVREFLLKTC
                     IVETLSGPLCEDLTDTTDGQEMLERLERANLFLVPLDEGGRYYRYHHLFAAFLHERLR
                     RAHPEAVPELHRRASLWYEGDGCLSAAIEHALDAEDFGRAAGLIQEETGVRRTYVDAS
                     LLLRWLGTLPDWLVRQQPQLCLLYAWALTHSGELEDAELRLRDTEAALKLEDGVSTDA
                     LTDEERTMLGEICIVRARVAAMRENAPLTTELSNRAL"
BASE COUNT         2219 a         3584 c         3424 g         2162 t
ORIGIN      
        1 cgggttgcat cgtctccctc acgaccccta ccatctcgtc gctcaatgcg tccaggtcgg
       61 tttcctccct gagcctggtc gagaactctt ctagggtctt tctcgcatcg tacttgcgtc
      121 tgtagaagcg cctatctatg aacgactgaa tgcgatgcct tagagggttg aacagcgcgg
      181 ctatcacaag tgtggatacg actatggtga gctgcggaag ctcttgctgg ccggtgagcg
      241 cctggaaaat cgcctgggta accgttacac ccccaaagta gaccagcact agcaaggcgg
      301 tgagcaaacc gtagacgaga gttcggttta tgagaacgtc tatgtcgaac agacgcgagc
      361 gcaacacggc cacgccgatg gacaacggga cgagcagtaa ggataacgcg aagcccgcat
      421 tgaggacgag taggacgaaa ggcgtgtctc cgccgagcaa tgagaggtcc aaaggcagtt
      481 agaaggggaa ggatgccgcg acacccaagg tcgtgccgaa cacgacccac ctggtttgcc
      541 gacgttgcgc ctcagaggag acttcacggt aacgatagat ctgggaccag accaggctca
      601 ctatcatccc tgagaacacc cccagtgaaa ccatctccag gacgggcgaa cgagagtaca
      661 ggtcaggaaa ccgatcgcgg gaaacctcaa aagcgatgaa cacgacggcg agccagcgcg
      721 tccagcgcgg cacgaagcgt cctccgggga acagcaagaa gaatagcacg acgcaaaccg
      781 cgcccattat ctgcacgccc tggacgggca accaccaagc cggctgagag gagaccaggg
      841 cgtcggcatc cgtggtgtcg accgtaaccg tcccgaacga cactagaaac gccgagacca
      901 gtagcgccat ccggtcgtcg gagcgccgcc agaagatgag cgcgcccaac ataaaccaca
      961 ccaactggaa acccttgtcg atgaggacgt tcaggagcgc gtaggagcgt accgacaggc
     1021 ctacgtcccg taacgctcgc acgccttctg gggtaggctg ctctatcgcc cgctcggaac
     1081 agacctcgga tgcggcggtg cacacgctac gatattgctc taagctgctc gggacgctga
     1141 tgaggactat cgcaagcgcc gtgatggcca ccgctgccca aagtaggcgc gcaaagggga
     1201 gccagcgccc gctgagggta gtggcaggtg gcgaggcacg ggtgctcatc tcccgactcc
     1261 tcgatgctgt taccttgccc tttccgtcac aacaccactc cacagggcga ctgtctccca
     1321 agcgttacga caaacgttac gaagccgagc ggtgagcaaa cctcggagaa tccaccttct
     1381 acgcacacct ataggtgcga aagcccaaaa tcgtgtgtcc ttgtgtttcg tgccttgctc
     1441 atggtgagca gcgatgagcg cttggaatta tcgaggagct gacggcagag gaaatacact
     1501 gtctgggcga aataatggct gctgtggcaa aagagagctc gggacattgt gctaagttcg
     1561 agctggtgca ccaccgcgtc ataggctcgt agtgggtcca tctgacaggc ttcgtgcagt
     1621 atttcatgcg gacatccgaa atggctacgc acggttgaac gtatgttcga gccaacgtca
     1681 cgccgtgggt gacggacacc cactacgtca acttcatcat ggagttcgta ctccagactt
     1741 caaccttgtg ccttacttat gaggtgcagt acgctaaagg gtaaagaaaa aggtggcacc
     1801 cttcccgacc tcgcctttgg cccacactcg gccaccgtgc cggtggatga tgcgctgcac
     1861 cgtagccagt cctattcccg taccctcgaa ttcgcccatg ctgtgcaggc gctggaaagc
     1921 cccgaacagc ttgtccgcgt aggccatgtc gaatcccacg ccgttgtcac gtacaaaata
     1981 cgcctgcgtg ctctcatact ccagggttcc caactctatc cgtgggtgcg tctggtccct
     2041 ggtaaacttc caggcgttgc caagcaagtt ctccagtacc accctcaaca acctcccgtc
     2101 gccattggct acgagtccct gctctatgac tacctccact tgccgtcctg gctggactct
     2161 tccaagctca ttgacgatct tcctggccac gtcgctaagg ttcacccttt cccggcccat
     2221 ctcaccgcgc gtcacgcggg agagatccaa gaggtcgtcg atgagttgcc ccatccgctg
     2281 gctcgcgtcc ttggtgcgcc tcaggtaatc tctaccttcc tcgttgagct tctctttgta
     2341 gtcttccagc agtatttggc tgaagccgtc gatggccctc aagggggcac gcaggtcatg
     2401 tgagactgag tagctgaagg cctccaactc gctgttgaag gcctcgagct gggctgtgcg
     2461 acgccggacg cgttcttcca actcttcgtt gaaccggcgg agttcttcct cggcgcgctt
     2521 acgctcggta gtgtcgcgaa tcgaggcgaa gataaggtgt cgtccctcgt actcgatggc
     2581 tccgacgccc acctcgaccg ggaagattgt gtcatctttg cgccgcagct cattctcctc
     2641 aaagcctacg atcttgcccg gctcgccctg aatagctcgc tcccatagcg tgttgccctt
     2701 cctctctcgc cgctcctctt ccgagatcag gtgcgtcgct acgtccgcaa ccgagaggag
     2761 cagcagttct tcccgtgtgt atcccagtgc ccggcacgct tcggcgttgc agtccacgaa
     2821 gcgaccgtgc tcgtcgtgga cgaaaagaac gtcggccgag ttctcgaaga gctgcctgaa
     2881 tcgcctctca ctggcgatca gcgccttctc ggtttgcttg cgctcggtta tgtcgtgggc
     2941 gacgacgcac atggcctccc tgccacgata aggaataacg ctgacactga cttcgacatc
     3001 agcgagcgag ccgtccttgc gccggaagcg cctctcgccg atgaagtggt gacctctgtt
     3061 catgattcgc cgaacactgt cgtccacgct ttcttggtcg tgggcaacta tgttgtagag
     3121 tgtcatccgc agcagctcct gctccctgta gcccagcgat tcatagaaag ctgcgttggc
     3181 ttcgaggatc tgcttggtct cggcgtccac gaggaagatg ttctcggcgg cttgctctac
     3241 caccgtgcgg tagagctgct cgctctcacg aagccctcgc tctcgctcct ccagcttcgt
     3301 gagggctgcc tgcaaccgca ccgtgcgctc cgcaacacgt tgctcgaggg tttcattgag
     3361 ccgccttact tcttcttcgg tccgcttgcg ttcggtaatg tcgaagatca cgccctgcca
     3421 gaaccttggg cggtcttcct cgtcccaaat gatagaagcc tcgtcgcgca gccacaccac
     3481 gcgaccatcg ctggccaaca cgcggtattc cagcttgaac tcgccgcgct cggtggccgc
     3541 cacctcctct gccatcacgc gcctgcggtc ttcagggtgg agaattttca cccaatagtg
     3601 aggatccttc tcgtaggtgt ccgcagggta gcccagcata gcctctatct gcgggctgtc
     3661 gtagagggtg gcctgggtct cgacgtcttc gatgtagacg atggcaggga tgtgctcgac
     3721 cagggcacga tacttgaaat ccgcctcttg ggccctaatc tccgcctgcc tgcgcttggt
     3781 caagtctcgg gaggtaacca ccacacccat cacagccggg tcgtcgagca ggttattgac
     3841 gatgtgttcg acgttgcgcc aagagccgtc cttgtgcggc actcggaact cgatgggcgg
     3901 gtgaagccca ggtcttttca ggacatcggc gaagatgccc aatgcgcgct ccctgtcgtc
     3961 cggatgtacg gcgccaaacg cgttggtccc tatctgctct tcaggcatgt agcccgtcac
     4021 cctttcgacc gccgggctta tatagcggac ggtgccgtcg gcctccagga gcgtgatgat
     4081 atcggaggtg ttctgtatca atgtgcggaa gtgctccgct ctctcctcga tagcattctc
     4141 gacctccttg cgctcagtga tatttcggac gatggcgagg acgaagcgcc cgtttctcac
     4201 ttctgagtcg cgtatcggct cgatggggct gagggctatc tcgatggcga tctcctcgcc
     4261 gtcctttcgc acagcaggca actccagcac cgcgtgagaa tcgatgtagg ggccgtggcc
     4321 cgtatcccga tagcgggcca tgcctgcctc gcactgcgcc ttgagtcgtt cggggacgat
     4381 cacggtccaa ctatgctcga gtgcctcaga ggaggagtat ccgaagatct tggtcgcggc
     4441 tgcgttccac aggactatcc tgccgctgcg ggcgtctacg acgaccaccg cgtctcggat
     4501 gctctcaaga agttggtcta tgcccaagtc gtgtagtcgc acggctagat cacaccacat
     4561 atacggcgga cgatgttagc gttcctcgaa cgtcgccctg ccgcccttcc cgcctctgca
     4621 ttgtgctatt tttgaagctt acataaggtt tctggggttc gcaatctgac gtctgccgcc
     4681 gacctgtgtt cgtcataagg tgatctttgt accctagctt cgggagggtt gcacgcggtc
     4741 caggtgccga tcaggatggg aactcgagta atggtcatgt cgtcgatgag gcccgcgttc
     4801 agaaaccctt ggatcttctt gcctccgtca acgtagaggt gtgtcgcgcc ttgggccgcc
     4861 tgctagggtg cctcgcgggc ggggaggtca ggatgaagga acgaggaaag gatcgatatg
     4921 ggacaactca tcacggacct gttcatcaca ctcgacgggt acgcctccgg cgaaggggcg
     4981 ccggcttact acggttacca cggccccgaa ctcgagcgtt gggtggacga gcacctggca
     5041 acgccccagg tcctgctgat ggggcgcgtc acctacgagg tgctctcggc catcgcccag
     5101 aaccatcccg ttgagggccc cgaccggatg agcgagctgc caaaggtggt gttctccagg
     5161 acgctgcgag agcccttggc ttgggacaat gccagggtgg ccagggcgga cctagttgag
     5221 gaggtccgga ggctgaaggc cgaaagcgac gccccgctgc gtaccatagg cagcctctcg
     5281 gtggtcaagg gcctcttgga ggcggggatg gtcgatcggc tccggctaac cgttttcccg
     5341 ctgattctcg gcagcacggg ccgggagccg gtcttcgcag gactccccga catccacctg
     5401 gagctggtcg acaccgaagt cctcgatgcc cgcctcgtca cgctcgagta tcatcccgct
     5461 accacccagc agacggacta agtcgaagca accgcaagca ccgtagccgc tcaagggtgc
     5521 cgcccctatg tgggggtggc gagatcacgt ttcgccaagg aggaccaggc ggcgacccgg
     5581 agtagcaacg gactattcac ttgagtatct acagcggctt ggggaaggat tgatcctccc
     5641 acaagccggg caacaggttt caggcatcag gaaggtcttc cctgactgac ggtggtcgct
     5701 aaggtacgca tctggggcga agtgggtcaa tacttatgaa ccgcgctgtt agaagtcagg
     5761 attctcggag gatcggaggc agggctgttc tcggaagtcg gcatagcccc gatccgcgaa
     5821 tgtcgctgtg aagaacgggt gcagcggcgc ccgtttagat gtacccgaac aacggcggaa
     5881 agacgatcac gacaatcgcg gcccacacgg cgtagactgg caagtaatcc gtctgccacc
     5941 gttcgaggcc cgtgaacgat ctgcgccctc tcagaaagcg gatgtagagc acgatagacc
     6001 acgccagatt gaccagaaga atcacgttct cacccagggc ggccacacgg ttcgggctga
     6061 agccgaactc ggtgatgcgt gcggcgatag cccacagcgc taccgcgtcg gccaatagcg
     6121 cactgaccac cagcacaacc tgcactacat cgaaggcgcc ggggggcgac cggggatccc
     6181 tggcggagac ggaatagagc agaagaccaa ggaccagcac caggagcagg tcgaaggcga
     6241 taagcacgct ccgctcgatg ccgactccgc gtccggtcca cagcagggtc cccagaaacg
     6301 tgaccagcac ggcggcgaaa aggggtgtga agaggcgcgt caaaacgggc gccatgttct
     6361 cgatcacgct ttgtttggcc tccaccagcc aggaggcgac catcacggcc ccgaccgccc
     6421 cgcacggcag cagccacgac tcgaagaagg gctcgacgtc gattccgata gactggaaga
     6481 tcatgaacat gaacgccatg aggacgccgc cgccgagggc gatcagcacg tagtagatga
     6541 acagctcgcc cgagaaccga atgaagtcca tgcggccacc aacctggccc caccggccgc
     6601 ccgcgtacgc gatgcccact accaaccaga gcgcgatcgg caggtgcagc gccgtaagca
     6661 cctcggtgta gccctccggg gcgaagggat agacgttggc gaatacaccc gccgcgacga
     6721 acgcgacggc cagccagagc agagtgctgg tcccgagccg ccgcttccag acgaaatagc
     6781 cggtcagaag gggcagcacg aagagactca gattgcgggc gtagaagcca gcgtcctggt
     6841 ccagttcgat cccgaagagc gccggcacct tgatgagcaa ggccgccgcc accgcaagac
     6901 agaagacgac gatggcatcc gttctggccc gcgctcgtgg ctccccggag cctgaaggga
     6961 caacgacgag ctgcttccag aggcgctcgg agtgctcgcg cgcgaactcc cgtgagaggg
     7021 cgtcgagttt acccatgcgc ttcaccgcca ccaggaacgc ctcatcggtg gcgagccccg
     7081 cgtcgaccag caccgctatc tgctcgcgca ggtggtcttc cagctcctct acgtcggcgg
     7141 agtggatcgc ctgccggcgg cggaggtagc tccgccattg gtcgatctgc tcctcaagcg
     7201 atggccggtg acctggatcc ggcatcgtca gactccctgt ggaagtggcg cggctgacgc
     7261 cgggaggctg gccggaactg gatgggagtg cgcctgccag atgccgcgta gcgtattgtc
     7321 caccgcttgc cattgcctgc gttcctcggc gagctgatcc cgacccccgg gcgtgatgcg
     7381 gtagtacttg cgacggcgac cgcttcccgc aacttcccac cgcgcctcga cgtggccgag
     7441 ccgctcgagc cggtgcagaa cagggtagag catcccgtcc gtccactcca tgcgccctcc
     7501 tgacaactcc cgaactcgct ggaggatcgc gtaaccgtag ctgtcgtctt cggccaggat
     7561 cgccaggacg atcggagtcg aggaagcggc tatcagatcc ttgttgatct ccatgatttc
     7621 acgcgtcatg agacgctaat gacctccaac ttcattgtac atagcagcat tatacatagc
     7681 agtactaggt atgtaaagaa ccgcggtttc gaggaggatg gcagagggac aattccccgt
     7741 cttgacagcg gcttggggaa ggaatgatcc tcccacaagc cgggcttaag gcaacaggtt
     7801 tcaggcatca ggaaggtctt cccggactga cggtggtcgc taaggtacgc ctctggggcg
     7861 aggtgggtca atacttatga accgcgctgt gagaactaga gcggtttgcg gaaagagtga
     7921 acctgaggtg agtaaaggtt tcaggcatca gcaaaaagac ctgtagcctg atgccttctc
     7981 cgaaggagta gctcatcctt ttcgagaact gccataaggc atcgaggatc gcttcatagg
     8041 taacccgccc gaacaccatg gagtcggctg cggccatcac cgctccgatc gcctctccca
     8101 tctcgtcgtt gaagtacgag aggtgctact tccccggcga atccgtgacg ccgtccggcg
     8161 agaccaactc cgatgcgact actttctcat agcaacctct gtgaggtaaa aggctctgga
     8221 gacctacgac accgcttcgc tcgtgggctg tgcctcaggc cttatgttct ggttgagccg
     8281 gaagaagttt gtagggtcgt agcgaccctt gatcgcagtg agcatctcta gggtcttccc
     8341 cgggtaagcc tcacgcacgc gcccctcgcc ttcgtcaccg aggaagccaa cgtacgcacc
     8401 gacttcatcc ccccggtgta gagcggccgc aaaatcggtg acccagggct cgtagaccgc
     8461 cgcctcatcg gggtgctcgt agagcgcggc gacgttgacc atgatgcgcg agccgcggtg
     8521 tgcaaacgcg gtggcctctg cggggacgcg cgccatcgcc cctccgagta cccggatctg
     8581 ggccaccgtc atctgggcgc tggagttctt gaggtggtcg actatcgtct gggccaccgc
     8641 gccatcgatg gtctccaaaa acatcgtgcg tcccgagccg atcggatggt agccgtcctc
     8701 ctcgggtggg tagatctccg ggtaaggcat cggcttgacc atatcggcga tcggctcggc
     8761 gagcccccga aacggcgcga tggcgcgctc gccggcctcg acctcacccg cgtagaccag
     8821 caatgccatg ataatgagct ggccgtgcat ctccgcgggc acgaacggca tcggcggtgc
     8881 cgtcatgacg ttggcgatcg cagatagctc ctccggagcg gcctccgcct cggcgacgaa
     8941 cgaggcgacg acctctggag tcgcaggcag gatcagcatg cctcccacga tcgtatcgac
     9001 ctcgtgcagc cgatactcaa accgggtcgc cacgccaaag ttccctccgc caccgcggat
     9061 cgcccagaac aggtccgggt gcctctcggc gtccacccgc aggagctgtc cgtccgcagt
     9121 tactacatcc gcggccagca ggtcgtcgat cgttaggccg tgcttgcgaa cgaggaatcc
     9181 aaccccgcca cccagcgtga tccctccgat cccgaccgag ccagtgtcgc cgaagcccgt
     9241 cgccaggccg tgggctccag ccgcagcggt gtattcgcca gcggtcaggc cagtctcggc
     9301 ccacgctgtg cgccgctcga ggtcgatctc gagccccttc atctgcgaga ggtcgagcaa
     9361 gatcccaccc tcggtggtgc tgtgcccggc agggctatgg ccgccgctgc ggactgcgag
     9421 ttccaatccg ctttcgcgcg acaaggcgac gacacgcgag acgtcggtcg cgtccgcggc
     9481 ccggacgatg agcgccgggt ggcggtcgat cccgccgtag aagacggcgc gcgccacgtc
     9541 gtaggcgtcg tcatcggggc gtatcagctg gccccgtacc ctggacgaaa acgcccttat
     9601 ggccacctca tcgaggagcc tgtctcggtg cgatcccagg gcatcttgtc gattgtcagc
     9661 catgatcctg gctccgatcc ttggtttact tctagtaaga aacatactgg ctagccgggt
     9721 agagacatcc cccaagagag ggatcagggg gcggagtttc tagcggccta tccgtcgcgt
     9781 gtgccgagat cggacctcca cgatgttctc cgggatcaaa ctccagctcg aattcagcac
     9841 gccagagaac gagtagccgt accgttggcg tacctcggga gtccgcgcat catcgagtac
     9901 aataagcctt gtggtagagg cgaagctcat ggacaggcag atactcaggg ccaagctcta
     9961 cgtaccccgc gcccgcctcg acaacgtggc gcgcccgcgc ctctacgagc ggctggacga
    10021 gggcgtacgt cgcgagttga ccgtcgtctc tgcgccggcc ggcttcggca agacgacgtt
    10081 gctcgcggac tggagccggc gcggcgaact tccggttgcc tgggtctcgc tggacgagag
    10141 ggacgacgat ccggttcgct tcctcttgta tttgatagcg gctatcggca cgatccacca
    10201 gggcttcggg gaaactacgc gcgctttcct gagctccctg ctgtctcagg aggagctcga
    10261 gcccgtcctc accgcgctca gcaacgaaat cctggagttg cccagcgatt tcgtactcgt
    10321 gctcgacgac taccacgcca tacgctcgga ggccgtccac gacgccctgg cctttctgct
    10381 tgaccactca ccgccgccga tgcaccttgt tctcgcaggt cgcacaagtc ctccgttgcc
    10441 gcttccacgg ctgcgcgcac gaggaagatt gaccgagctc ggcgtctccg acctgcgctt
    10501 caccctcgaa gaggcgtcag attttctcgg ccgcacgatg gacctgagcc tcaccgcaga
    10561 gagggtcgca acgctggaaa gagggacgga gggatggatc gtcggcctgc agttggcggc
    10621 gcacgccctc cgggggggcg acgccaggcc cgtcgaggct ttcacaggaa gtgcccgcca
    10681 cgttgtcgat tacctggctg acgaggttct ctcccgccag ccggaggaag tgcgggagtt
    10741 tctgctcaag acctgcatcg tcgagacatt gagcggtcca ctgtgtgagg atctcactga
    10801 caccaccgac gggcaggaga tgctggaaag gctggagaga gccaacctct tcctggtgcc
    10861 gctggacgaa gggggacgct actaccgtta ccatcacctt ttcgccgcct tcctgcacga
    10921 acgcctgcga cgcgcgcacc ccgaagcagt tcccgaactg catcgccgcg ccagcctctg
    10981 gtacgagggc gacggatgcc tctccgcggc gatcgagcac gccctggacg cggaggattt
    11041 cggccgggcg gcgggcttga tccaggagga gacaggtgta cggcgcacat acgtagacgc
    11101 ctctttgctg ctccgctggc tgggaacgtt gcccgattgg cttgtgcgcc aacagccgca
    11161 gctgtgcctg ctctacgcct gggcgctcac gcattctgga gagctggaag acgccgaact
    11221 ccgcctccga gacaccgagg ccgccctgaa gctcgaagac ggagtttcga cggatgccct
    11281 cactgacgaa gagcgcacga tgttgggtga gatctgcatc gtgcgcgccc gtgtggcggc
    11341 catgcgggag aacgcgccgc ttacgacgga attatccaat cgggccctc
//
LOCUS       DATAIR010000779         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_225491, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000779 DATAIR010000000
VERSION     DATAIR010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225491"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VHM69_18460"
     CDS             complement(<1..369)
                     /locus_tag="VHM69_18460"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2742429.1"
                     /translation="MTWLLLLPVALAAGMAAPTQFAINSQLRQVVGGPVMAAALSFLV
                     GTVVLFAATAVVRRSVPELGPIMSAPWWMWLGGLLGAFFVVASIILTPRLGAATTVGL
                     FLAGQVIASIAIDHFGLLGVP"
     gene            complement(391..648)
                     /locus_tag="VHM69_18465"
     CDS             complement(391..648)
                     /locus_tag="VHM69_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742430.1"
                     /translation="MEREPLSPEAEALWRRLWEIWQDNDEEDVVLDSVELSDLAEEIP
                     GLENRMKTALAYLQRARYIQYRSGVGEDGIEPILYDVYEPR"
     gene            complement(653..1177)
                     /locus_tag="VHM69_18470"
     CDS             complement(653..1177)
                     /locus_tag="VHM69_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX2742431.1"
                     /translation="MSESVHKHRESAPETVRVAVLTISDTRTRETDTGGDTAQELLRE
                     AGQEIVARQIVRDEAASIRTNLIDLLARPDVDAVVTTGGTGISARDTTYEVVDRMIEK
                     KLEGFGEIFRMLSYEEIGAAAIMSRAVAGSVGTKFVASLPGSRNAVRLAVERLLVPEL
                     AHIVFELRKHQESR"
     gene            complement(1174..1806)
                     /locus_tag="VHM69_18475"
     CDS             complement(1174..1806)
                     /locus_tag="VHM69_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2742432.1"
                     /translation="MILEMLTVGPFQENCYIIGDKGVGALIDPGDEAARIALAVEQTN
                     LEISSIIVTHAHIDHVGAVAALVDEYSCPVLMHADAEPMLKQLPTQAMMMGLKFGKVP
                     KVDRHVEDEEVLEVGDLRLRSLYSPGHAPGHLAFYVEDESLVFSGDALFAGSVGRVDL
                     PGGSMEVLIRSIEERLLTLPDETRVYPGHGPQTTISNERTSNPFLQGGLL"
     gene            complement(1803..>2923)
                     /locus_tag="VHM69_18480"
     CDS             complement(1803..>2923)
                     /locus_tag="VHM69_18480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2742433.1"
                     /translation="RDVARAAAEGAKLGLYRFDRHKTAVKGHDLETFWLVAEDEALVE
                     AGEGAEVGESVAEGAVLARDLANEPSNVATPEYMAERTRGIAAKFGMELNVLDRAGIE
                     EEGLTGLATVGRSASNEPRFIVLEHRKGGDDAPVVLVGKAVTFDSGGISIKPSSGMED
                     MKFDMSGGAAVLGAMESVGALDLALNVVAIVPATENLPGGDAFKPGDVLEMQSGKTVE
                     IVTTDAEGRLILADALSYARRYEPAAVIDCATLTGACVVALGNHASGLMGNDEDLIAE
                     VQAAGDATGERAWPLPLFDEYRDQITGDTADIKNSGGRGGGALTAGAFLKEFADYPWA
                     HLDIAGTAYGKKGNAYTTKGATGVPTRQLVEFLIGRTR"
BASE COUNT          474 a          977 c          893 g          579 t
ORIGIN      
        1 ggggacgccg agcaggccga aatggtctat tgcaatagag gctatgacct ggccggcgag
       61 gaacaggccg accgtcgtcg cggcgccgag gcggggggta agtatgatcg aggcgaccac
      121 gaagaaagcg ccgagcagac caccaagcca catccaccac ggcgcgctca tgatgggccc
      181 cagctcgggc acggagcggc gcacgactgc cgtagcagcg aagaggacta ccgtccctac
      241 caggaacgag agcgcggctg ccattaccgg cccgccgacg acctggcgta gctggctgtt
      301 gatcgcgaac tgcgtcgggg ctgccatacc tgcggcgagc gccacgggga gcagcaatag
      361 ccaggtcatt ctcgtcactc ctcaagtacc ctagcgtggc tcgtagacgt cgtagagtat
      421 cggctctatt ccgtcctcac cgacgccaga gcggtactgg atgtagcggg cgcgctggag
      481 gtaggcaagg gccgtcttca tcctgttctc gaggcccgga atctcctctg cgaggtccga
      541 gagctccacg gagtcgagga ccacgtcttc ctcgtcgttg tcctgccaga tctcccagag
      601 tctgcgccag agggcctcgg cttccgggga caggggttct ctctccatct ctctacctgc
      661 tttcctggtg cttgcgaagc tcgaacacta tgtgcgccag ctcggggacg aggagcctct
      721 cgacggccag cctgacggcg ttgcgcgatc ccgggaggct cgcgacgaac ttggtgccaa
      781 cggaccctgc tactgcgcgg ctcatgattg cggccgcccc gatctcctcg taggagagca
      841 tcctgaagat ctcaccgaac ccttctagct tcttctcgat catgcggtct acgacctcgt
      901 aggtggtgtc gcgagccgag atgccggtcc cgcccgtagt cacgaccgcg tcaacgtcgg
      961 gcctcgcaag gaggtctatg aggttagtgc gaatgcttgc ggcttcgtcc cttactatct
     1021 ggcgggccac gatctcctgc cccgcttccc gcagtagctc ttgcgccgtg tcaccccctg
     1081 tgtccgtttc gcgcgtcctc gtgtcactaa tggtcagtac ggcgaccctg acggtctccg
     1141 gggccgactc cctgtgcttg tggacgctct cgctcacagc aggcccccct gcaggaacgg
     1201 gttggacgtc cgctcgttcg agatcgtcgt ctgcggcccg tggccggggt ataccctcgt
     1261 ctcgtcaggg agggttagca gtctctcttc gatgctgcgt atgaggactt ccatgctccc
     1321 gcccggaagg tcgactcttc ccacgcttcc agcgaacagc gcgtctccgg agaacaccag
     1381 actttcgtcc tcgacgtaga aagcgagatg tcccggcgcg tgacccggcg agtacagcga
     1441 gcgcagcctc agatctccga cctcgaggac ctcctcgtcc tcgacgtgac ggtctacttt
     1501 ggggaccttg ccgaacttga ggcccatcat catcgcctgg gtcggaagct gcttcagcat
     1561 cggctctgca tcggcgtgca tcaggaccgg gcaagagtac tcgtcgacca gcgcggcgac
     1621 ggccccgacg tggtctatgt gcgcgtgggt gacgattatg gaacttatct ccaggttcgt
     1681 ctgctctacg gcaagcgcga tgcgggccgc ctcgtcgcca gggtcgatga gcgcaccgac
     1741 gcccttatct ccgatgatgt agcagttctc ctggaaaggg cccacggtga gcatctctag
     1801 gatcatcgcg tcctccctat aaggaactcg acgagctggc gggtcgggac gcctgtcgcg
     1861 ccctttgtag tgtatgcgtt gcctttcttg ccgtaggcgg tccccgctat gtcgagatgt
     1921 gcccacgggt agtcggcgaa ctccttgagg aaagcgccgg ccgtcagagc gcctcctccg
     1981 cggccgcccg agttcttaat gtctgcggtg tcgcctgtga tctgatctct gtactcgtcg
     2041 aagagcggca gcggccacgc cctctctccg gtcgcatcgc ccgcggcctg aacctccgct
     2101 atgaggtctt catcgttacc catgaggccc gaggcgtggt tgccaagcgc gacgacgcag
     2161 gcgcccgtga gggtcgcgca gtcgatcacg gcggcgggct cgtagcgcct ggcgtaggag
     2221 agagcatcag ccaggatcaa acgcccctcg gcgtcggtgg tgacgatctc gacggtcttt
     2281 cccgattgca tctccaggac gtcgcctggc ttgaaagcgt ccccgccggg caggttctcg
     2341 gtcgccggca cgatggcgac gacgttgagc gcgagatcca gggccccaac cgactccatc
     2401 gccccgagta ccgcagcacc cccgctcatg tcgaacttca tgtcctccat gccggaagat
     2461 ggctttattg agattccccc tgagtcgaat gtaacggcct tcccgacgag gaccacaggg
     2521 gcgtcatctc cccctttgcg gtgctccagc acgatgaacc gcggttcgtt ggaggcggag
     2581 cgcccgaccg tggcgagccc tgtgagccct tcttcctcga tgccggcccg gtcgagaacg
     2641 tttagttcca taccgaactt cgctgcgatt cctcgcgtcc tctcagccat gtactcgggg
     2701 gtcgcaacgt tggacggctc gttggcgagg tcgcgtgcga gcacggcccc ctctgccacg
     2761 ctctcaccca cttcggctcc ctctcccgcc tccaccagag cctcgtcttc tgcgacgagc
     2821 cagaaggttt cgagatcgtg acctttcacc gccgtcttgt gcctgtcgaa tcggtaaagt
     2881 ccgagcttcg cgccctctgc ggcagcccgc gcgacatccc gtg
//
LOCUS       DATAIR010000780         7198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_269905, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000780 DATAIR010000000
VERSION     DATAIR010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269905"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHM69_18485"
     CDS             <1..189
                     /locus_tag="VHM69_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX2742434.1"
                     /translation="LDRPRSHYVELLEAAGGTFTSSVSKNTDYVLAGEEAGSKLERAR
                     ELGVPILDEAGFEELLSS"
     gene            complement(467..1426)
                     /locus_tag="VHM69_18490"
     CDS             complement(467..1426)
                     /locus_tag="VHM69_18490"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2742435.1"
                     /translation="MERLRRNLVLALALGAAVYLILAILSDLDELAAALDSFNYALIP
                     VILGLVTLSYVGRFFRWVYYLKVLKVSVPIGMNAAIFASGLSMTISPGKLGEVLKSVF
                     VRQVSGAPIARTAPAVVAERATDGTGMIAWGFLGAFALNVPPWTMLAFLAVAVFGIAV
                     LRSKRLSLAAENILSQLPLVNRFAPHLGDFHGASNELLGTGPLVVGTAISFVAWGLEC
                     LGVYMCAVGLGADRPFLLIVFVFATSSLLGVVSMLPGGIGAVEAGLYGQFVTVANLST
                     GMAGALTLVIRLATLWFATLLGICGLLLFRRLLGDAPLEPGKP"
     gene            complement(1414..2337)
                     /locus_tag="VHM69_18495"
     CDS             complement(1414..2337)
                     /locus_tag="VHM69_18495"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX2742436.1"
                     /translation="MVETAVVKADRPRGLARSYLRLVRPKQWTKNGFVLAGVVFAEKA
                     FSTTAVAHALLAFVVFCALSGAVYAANDVLDVEEDRKHPLKRFRPVASGDISPRAALA
                     FAAVLAAGALVLSFTMLPVGVGIAGVAYLVLQVFYTTILKHTAILDVMSISAGFVIRA
                     LAGVAAVESPISPWLIVCTGLLTLFLGFSKRRHEIATLGEGAVVHRKNLRDYSVPMLD
                     EMMNIMISATIIAYTLYTFTVYERQDQIFMMGSIPFVVYGVFRYMLLVHRDGGGNPDT
                     LLLSDRPLQIALFLWLVVVGAVIYVIPLWNG"
     gene            2560..3459
                     /gene="menA"
                     /locus_tag="VHM69_18500"
     CDS             2560..3459
                     /gene="menA"
                     /locus_tag="VHM69_18500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX2742437.1"
                     /translation="MNSTRRQVLAEWFRLARPFSLTAAAVPVLFGTALAYKDGEFAWG
                     PFLAMLFGSLLIQAATNMFNEFYDEQRGLDVAGTVGIAGSIVSGRVSSRGVLIGALIC
                     YTVALFLGIYLIYVGGWPILVLGCLSALGGYLYSAGPRPIAYTPASEAAVFVFMGVLI
                     VVIAYAVQAGTFPLYVPLAALPIGGPVAAILLANNIRDLTSDRRGGRRTLPIVFGREV
                     AILIYRALLLEAYVSVAVLIVFGVVPPECALVFPSVFAAFRLWRDIAAYSTPRRLDPV
                     VKRTAGLHLVFGLLYTAGVLIGA"
     gene            3452..3928
                     /locus_tag="VHM69_18505"
     CDS             3452..3928
                     /locus_tag="VHM69_18505"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2742438.1"
                     /translation="MPENHRPFRASYLISGVAAERIFTALLDVRRFPEWVAGLHSSRA
                     LDPTDSTEVEEIRPAVRLEFVLSAAGLTHRVLSVVTVVEPPNRLEWRYVEGASGRGGW
                     LVEKEGVGSVRITLSTDYRIRPAWLDGMAHKPFFRGLAEDLIRRSMRRFEAHVRKD"
     gene            3988..4410
                     /locus_tag="VHM69_18510"
     CDS             3988..4410
                     /locus_tag="VHM69_18510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2742439.1"
                     /translation="MATVFTRIIEGELPGRFVWKDERCVAFLSITPLKAGHTLVVPRE
                     EVDHWLDLEEDLAAHLLSVSRSIGKALQKGFQPAKVGLLIAGLEILHTHIHLVPIDGL
                     HDLDFDGQERDPDPAELDEAARVIRGSLRELGFEEVAD"
     gene            complement(4468..5274)
                     /gene="tatC"
                     /locus_tag="VHM69_18515"
     CDS             complement(4468..5274)
                     /gene="tatC"
                     /locus_tag="VHM69_18515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2742440.1"
                     /translation="MTLIEHLGELRSRIIKVAIAFFLVSILAFLFIRRIFDFLLAPAP
                     QLKGSLFVTGVTEPLITDIKLALFTALLLTIPIILYQGWAFVAPAVGDMGRAFTYILI
                     TLASSLFLAGVAFGYYVVLPIGLDFLFDYGGNRYSEIISGEKYLPFVTRFLLAFGIVF
                     EFPAATYVGAKMELVDAPLLKKYRKHAIVLNTVLAAALTPGQDPFSMVLMAVPMVVMY
                     ELSIIIARYVNPTTEVTARELVRDDEDEGEERFDDERADDHPENGHERDL"
     gene            complement(5349..5627)
                     /gene="tatA"
                     /locus_tag="VHM69_18520"
     CDS             complement(5349..5627)
                     /gene="tatA"
                     /locus_tag="VHM69_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2742441.1"
                     /translation="MPGLGSFGGMELIIALVIILLLFGAKRIPELARGLGSGVREFKK
                     GTQDGEIEDKKKKEQEKKDEEQIESSKDEAKLEDDSTVEAEKAEQKRS"
     gene            complement(5695..6336)
                     /locus_tag="VHM69_18525"
     CDS             complement(5695..6336)
                     /locus_tag="VHM69_18525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2742442.1"
                     /translation="MMMNRDKLSFWVRLVSIVLAAFFLISFIFLGVGTNISYNVFELF
                     GNQDQPQKADQTPDTEEQIATAEKDLEKDPKNPEKIKDLAALYYTAGRYDDAVRVLEK
                     GREVAPKDEEIPMLLGQIYTQQAQMEQGDKQKEFHKKAGDSFAAAAEVEPDNAEAYLL
                     AGQAYDQAGQPAEAIKYYNGYLDLEPEGETAKEVKDRISVLLEGGESSGSAQP"
     gene            complement(6359..>7198)
                     /locus_tag="VHM69_18530"
     CDS             complement(6359..>7198)
                     /locus_tag="VHM69_18530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2742443.1"
                     /translation="AESGMAGTVYAEFFPHEIGTPEEAVEVIIGKVRELQKGLPERVS
                     ASVSVHSPYTVDPESSRLAARRTRELGWKLAIHLSESPEEVEFVRDGTGGLADIFGSN
                     EWGGAGVSPVRYAESIELLAPYTIAAHLATGVTEEDIEILARTGVAAAHCPRSNDFLG
                     CGVAPVAGMIERGIRVGMGTDGLWSSTSMNLFEETLFALDLHGMSGEAGLRLATLEGA
                     RALGIEAETGTIEAGKWADLAMVEVVPWGEDPERDVIEAAADGEVAATVVSGNLIYNR
                     LGA"
BASE COUNT         1359 a         2251 c         2210 g         1378 t
ORIGIN      
        1 ctggaccgtc cccgcagcca ctacgtcgaa ctgctcgaag cggccggggg cacgttcacc
       61 tcctccgtca gcaagaacac agactacgtg ctcgcgggcg aagaggccgg ctcgaaactc
      121 gagagggcca gagagctagg cgttcccatc ctcgacgaag cgggattcga agagctgctc
      181 tcctcctgaa cagcaggatt gataccgaat ccggttgaac gccgtcgctt ttcacccccg
      241 cggtagccac gccttcccgt cattcccgcg aaagcgggaa tccacgcccc ttggagagcc
      301 gtcatcggga gcgactgagg acggtcgcgt cagccaggca tggagcgctc ttatcccgga
      361 ttccgtatca gtcaccagag tcgtttccgc caatctccgc tgcagcccgg cgttgcgcca
      421 ttcttggccg agtttgtgta tacggggtgc ggtagcgttc agggtgctat ggtttccctg
      481 gctccagggg cgcgtcgccg agcaggcgtc taaagaggag caatccgcag attccgagca
      541 gagtggcgaa ccagagggtt gcgagccgta tcacgagggt cagtgcgccc gccattcctg
      601 tcgagaggtt cgcgacggtg acgaactggc cgtagagtcc tgcctcgacc gctccgatcc
      661 ctcccggcag catgctcacg actccgagaa gagagctggt tgcgaacacg aagacgataa
      721 ggaggaaggg acggtccgcg ccgagcccca cggcgcacat gtataccccc aggcactcca
      781 ggccccaggc gacaaaggag attgctgttc ccacgacgag cggccccgtc ccgaggagct
      841 cgttcgaggc cccgtggaag tccccgaggt gcggggcgaa ccggttcacg agcggcagtt
      901 gcgagaggat gttttccgcc gcgagcgaga ggcgtttaga gcggagtacg gcgataccga
      961 agaccgcgac ggccagaaac gcgagcatcg tccatggagg cacattcaag gcgaaagcac
     1021 cgaggaaacc ccatgcgatc atgccagtcc cgtccgtcgc gcgttccgcg acgactgcgg
     1081 gggctgtacg tgcgatcggg gctcccgaca cctgccggac aaagacgctc ttcagcacct
     1141 caccgagctt gcctggtgag atcgtcatag agagccctga ggcgaagatc gccgcgttca
     1201 tgccgatggg gacggagacc ttcaggacct tgaggtagta gacccagcgg aaaaagcgcc
     1261 caacgtagga gagggtgacg aggccgagga tcactggtat gagcgcgtaa ttgaagctgt
     1321 cgagggcggc ggcgagttcg tccaggtccg agaggatggc caggatcagg tagacggcgg
     1381 cgcccagggc gagggcgagg accaggtttc ttctcagccg ttccacaggg gtatcacgta
     1441 gatgaccgcc ccgaccacga caagccagag gaacagcgcg atctggagcg gcctgtcgga
     1501 gagtagcagc gtgtcggggt taccgccgcc gtcgcggtgg accaggagca tatagcggaa
     1561 gaccccgtag accacgaacg ggatgctccc catcatgaag atctgatcct gcctctcgta
     1621 gacggtgaag gtgtagaggg tgtaggcgat gatggtcgct gagatcatga tgttcatcat
     1681 ctcgtcgagc atgggaacgg agtagtccct caggttcttg cggtggacga ctgccccttc
     1741 gcccagggtt gcgatctcgt ggcggcgttt ggagaagccg aggaagagcg tcaggagccc
     1801 ggtacagacg atgagccacg gtgagatcgg gctctccacc gcggcgacgc cggcgagcgc
     1861 ccttatgacg aacccggccg agatgctcat gacgtcaagg atcgccgtgt gcttgaggat
     1921 ggtggtgtag aagacctgca agacgaggta agccaccccg gcgatcccta cgccgacggg
     1981 cagcatggtg aagctcagga cgagggcgcc ggcagccagc accgcggcga aggccagggc
     2041 cgcgcggggt gagatgtcgc cgctcgcaac gggcctgaac cgcttgagcg ggtgcttgcg
     2101 gtcctcctcg acatcgagca cgtcgttggc ggcgtacacg gcccccgaca gggcgcagaa
     2161 gaccacgaag gccagtagcg cgtgcgccac ggcggtcgtc gagaaagcct tctccgcgaa
     2221 gaccaccccg gccagcacga acccgttctt ggtccattgc ttgggccgga ccagccgcag
     2281 ataagacctc gcgagtcccc tgggacgatc ggctttcacg actgccgttt ctaccacaat
     2341 aggcttcagg tttcaggtta ggtttcaggc atcaggcatc aggcttcagg tttccgtcgt
     2401 cgaaagggca accgggcaac gttaccgtcc cgcttggtaa ccaccggaaa acccgagaag
     2461 acgctacgaa cgaacctacc tgacacctga tgcctgatgc cttactcgtg aacgtgccga
     2521 ttgcaaacgg cgacgactct ctccacaatt ggccggacga tgaactcgac gagacggcaa
     2581 gttcttgcag agtggttccg ccttgccagg ccgttcagcc tcacggcggc ggccgtaccc
     2641 gtgctcttcg ggacggcgct ggcttacaag gacggcgagt tcgcctgggg accgttcctc
     2701 gccatgctct tcggcagcct cctcattcag gctgccacaa acatgttcaa cgagttctac
     2761 gacgagcagc gcgggctcga cgtggctggg acggttggga tcgcgggctc catagtgagt
     2821 ggtcgcgtga gttcgcgcgg tgtcctgatt ggcgccctga tctgctacac cgtcgcgctc
     2881 ttcctcggca tctacctgat ctacgtcggc ggctggccca tactcgtgct cgggtgcctc
     2941 tcggcgctcg gcggctacct ctactcggca gggccgcgcc ccatcgctta tacgcctgcc
     3001 agcgaggccg cggtcttcgt cttcatgggc gtcctgatcg tggtcatagc ctacgccgta
     3061 caggcaggca ccttcccgct ttacgtgccg ctggcggcgc ttcccatcgg ggggcccgtc
     3121 gccgcgatcc tgctcgccaa caacataagg gacctgacct ccgaccgcag gggcgggcgg
     3181 cggacgctgc ccatcgtctt cggcagggaa gtggcgatcc tcatctaccg cgccctcttg
     3241 ctcgaagcct acgtttccgt agctgtacta atcgtcttcg gggtcgttcc ccccgagtgc
     3301 gcactcgtat ttccgagcgt cttcgcagcc ttccgcctct ggcgcgacat agcggcatac
     3361 tcgacgccaa ggcggctgga cccggtggtc aagcgtaccg cggggctcca cctcgtcttc
     3421 gggttgctct acacggcggg ggtcctcatc ggtgcctgag aaccaccggc ccttcagggc
     3481 gagctacctc atctccgggg tcgcggcgga gaggatcttc accgccctcc tcgatgtgcg
     3541 gcgcttcccc gaatgggtcg ccggactgca cagctcccgc gcgctggacc ctacggacag
     3601 taccgaagtc gaggagatac gtccagcggt caggctggag ttcgtcctga gcgccgcagg
     3661 actgacccac agagtcctca gcgtcgtcac cgtggtcgag ccgccaaatc ggcttgaatg
     3721 gagatatgtc gagggcgcat caggtcgcgg tggctggctc gtcgagaaag agggagttgg
     3781 aagcgtcagg atcacacttt cgacggacta caggatcagg ccggcatggc tagacgggat
     3841 ggcccacaag cccttcttcc gcgggctggc ggaggatctg atcaggcgct cgatgcgccg
     3901 cttcgaggcg cacgttagaa aagattagat aacacgtcct cagcgcagat ataatgctcg
     3961 atcgcaggca acagagggag gtcggagctg gcgacggttt tcaccaggat catcgaagga
     4021 gagctaccag ggcgtttcgt atggaaggat gagcgatgcg tcgcattcct ctccataacc
     4081 ccgctcaaag ccggacacac gctcgtcgtc ccgagggaag aggtggatca ctggctcgac
     4141 ctcgaagaag acctggcggc ccatttgctg agcgtctctc gctccatcgg taaagccctt
     4201 cagaagggtt tccagccggc caaagtcggt ctcctcatcg ccggcttgga aatacttcac
     4261 acgcacatcc acctggtgcc gatagatggg ctccacgacc tggatttcga cggtcaggag
     4321 cgagatcctg atccggcgga gctggatgag gccgcgaggg tcatccgcgg gtccctgaga
     4381 gagctcggct tcgaagaggt cgctgattaa agctatcagc caccagtatt tcagccagtc
     4441 aggaaaagct tggagcttag tcctgactta gaggtcccgc tcgtgcccgt tttcaggatg
     4501 atcgtcggcc cgctcgtcat cgaaccgctc ctccccctca tcctcgtcgt ccctcactag
     4561 ttcgcgggcc gtgacctcgg tcgtcgggtt cacgtaacgg gcgatgataa tcgaaagctc
     4621 gtacatcacg accatcggga cggccatcaa gaccatgctg aacggatcct gccccggcgt
     4681 cagagcggcg gccagtaccg tgttcagcac tatggcgtgc ttgcggtact tcttgaggag
     4741 cggggcgtcc acgagctcca tctttgcccc cacgtaagta gccgcaggga actcgaacac
     4801 tattccgaat gcaagcaaaa agcgcgtcac gaacggcaga tacttctcgc cgctgatgat
     4861 ctcactgtac ctgttgccgc cgtagtcgaa aaggaagtcg aggcctatcg gcaggacgac
     4921 gtaatacccg aatgccaccc cggcgagaaa cagcgacgag gccagcgtga tcaggatgta
     4981 cgtgaacgcc cggcccatgt ccccgaccgc aggggctaca aaggcccaac cttgatagag
     5041 gattatcggg atggtcagca agagggcggt gaagagcgcg agctttatgt ccgtgatcag
     5101 gggttctgta acgccggtta cgaacaggct gcccttcagt tgcggcgccg gcgccagaag
     5161 aaaatcgaag atcctgcgta taaataagaa tgcgagtatg gagaccagga agaacgcaat
     5221 tgcaaccttg atgatcctgg atctaagctc gcccaggtgc tcgatcaggg tcatccgcgc
     5281 ctcgtcgcgc gggcgcgagg gaagtctcag gcggccggcc atctgtagct taggagctcg
     5341 ctagggatct agctacgctt ctgctcggcc ttctcggcct cgacggtgga atcgtcctcc
     5401 agcttggctt cgtccttgct ggattcgatc tgctcttcgt ccttcttctc ttgttctttc
     5461 ttcttcttgt cctcgatctc accatcctgg gtgccctttt tgaactcgcg cacgccgcta
     5521 cctagacctc gcgcgagctc gggaatgcgc ttcgccccga agagcaacag tatgatgacg
     5581 agagcgatta tcagctccat gccaccgaaa gaaccaagac cgggcatctc ttcctcctaa
     5641 caagctatct gctggatctc acgcaacatt atacgccttc actgtgaaca cggatcaggg
     5701 ttgcgcgcta ccgctactct ccccgccctc gaggaggacg gagatcctat ccttcacttc
     5761 tttggcagtt tcgccctctg gctcgaggtc gaggtaaccg ttgtagtact ttatggcctc
     5821 ggcgggctgg ccggcctggt cgtaggcctg gcctgccaga aggtacgctt cggcattgtc
     5881 gggctccact tccgccgcag ccgcgaagga gtcccccgcc ttcttgtgaa actctttctg
     5941 cttatctccc tgctccatct gcgcctgctg ggtgtagatc tgaccgagga gcattggtat
     6001 ctcctcgtcc ttcggcgcca cctcgcgtcc cttttccagc accctaaccg cgtcgtcgta
     6061 gcggccggcg gtgtagtaga gggctgcgag gtcttttatc ttctccgggt ttttcgggtc
     6121 cttctcgagg tccttctctg cggtggcgat ctgctcctcg gtgtcgggag tctgatcggc
     6181 cttttgcggc tggtcttggt tgccgaagag ctcgaagacg ttgtaactga tattagttcc
     6241 gacacccagg aagatgaagg agatgaggaa gaaagcggcg agcacgatgg agaccaatct
     6301 aacccagaag ctgagtttgt cacggttcat catcatggcg gcttttcctc cgagatcgtt
     6361 acgcgccgag ccgattgtat atcaggttgc cgctgacaac agtggcagcg acctccccat
     6421 cggcggcggc ctcgatcaca tcccgctcgg ggtcttcgcc ccacggcacc acctcgacca
     6481 tcgcgaggtc ggcccacttt ccggcctcta tggtccccgt ctcggcttcg atgccgagtg
     6541 cgcgcgcgcc ttcgagcgtc gcgagcctga gtcccgcctc gccgctcatg ccgtgaagat
     6601 ccaacgcgaa gagggtctcc tcgaagaggt tcatactcgt gctgctccag agcccatcgg
     6661 tccccatccc gacccttatt ccgcgctcga tcatacctgc tacgggagcg acgccgcagc
     6721 ccaggaagtc gttggagcgg gggcagtggg ctgccgccac gccggtccgc gccagaatct
     6781 caatatcttc ctccgtgacg ccggtcgcca ggtgagcggc gatcgtatac ggagcgagga
     6841 gttcgatact ctcggcgtaa cgtaccggcg agacgcccgc ccctccccac tcgttgctgc
     6901 cgaagatgtc agcgaggccg ccggtcccat ccctgacgaa ctcgacctcc tcggggctct
     6961 cggagaggtg gatcgcaagc ttccacccca actcccgcgt gcggcgcgcc gccagacgag
     7021 aggattcggg atcaacggtg tagggggaat gcacgctgac actagcgctt acccgctcgg
     7081 gtaaaccttt ctgaagctcg cgtaccttcc cgatgattac ctcgacagcc tcctcggggg
     7141 tgccgatctc gtgcgggaag aactcggcgt agacggtccc agccattccg ctctcggc
//
LOCUS       DATAIR010000781         6285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_269906, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000781 DATAIR010000000
VERSION     DATAIR010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269906"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VHM69_18535"
     CDS             <1..756
                     /locus_tag="VHM69_18535"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742444.1"
                     /translation="IEMFDSLQQGLSDFFAFIPQLIGAIVILIVGYIVAKILQSVLAR
                     VLKAVGFDGWMERSGIKQFFDRAQTNQTPATILGKLVFWFVFIIAITMSTDALGIEQV
                     SAVFSQLIAYIPNIIAAVLILVLAGLLAQFVAGLVRGATGINILGTIAQAAIMVYAVF
                     AALTQLQIAVELTAPTFLIVLGAVALAAAIAFGWGARNVAQDIVESAYNRRDEARQKV
                     QEQGDSSATTGATGDGVLEDEDRLAVRPLRREE"
     gene            1183..4503
                     /locus_tag="VHM69_18540"
     CDS             1183..4503
                     /locus_tag="VHM69_18540"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2742445.1"
                     /translation="MARHHRVLAEVEHKFSETTIREPEAVRIGLLGGFRVSVGTRTIE
                     EGAWRLRKAASLVKVLALARGHRLHRERVMDLLWPQLGVKAASNNLRGALHVVRRTLA
                     SDTASRYLELQGDQLVLCPRGQLWVDVDVFEETAATARRSRDPAAYRAAIDLYAGELL
                     PGDRYEEWTERRREELRQLHLALLTEMAGLYEAREQYERGIEALRRVLSEEPAREEAH
                     TSLMRLYVLSGKRQESILQYERLRTSLTRELDVEPGIESRQHYEEIRAGESLATGSAG
                     DPSKRAADPAPHNLPASVTSFVGREREMIEARRLLSMTRLLTLAGAGGSGKTRLALEI
                     SRDLAGLYQDGVWFVELAPLSGPDLVAQEVAGALEVQERPGQPLTGTLVDSLRDREML
                     LVLDNCEHLIDAAARLADELLYSCPRLRVLATSRETLGIQGEVVWQVKPLSLPEADGG
                     ASVEGLMLYEAVRLFVDRARLRLPDFELMEANAGAVSRVCRKLDGMPLAIELATARMG
                     ALVVDQVAQRLEGSLDVLSAGSRTAEPRQQTLRATIDWSHELLSQTERALFRRLSIFT
                     GGWTLEAAEDVCSGESIYEGDVLDLLGSLVNKSLVVVAATGSAARYRMLEPIRQYARE
                     KLEESGEIDEMRSRHAAFFLALSEQAEPELSGTQQRMWVEQLEGEHDNLREAVSWVLE
                     REGAESALRFGGALWRFWFAQGHLSEGRKWMEQILAKGKPLSTLARVKALEGLGWLAQ
                     SLGDPGLVEATFEEMLSLSRKLGDKGNIATALNALSGQAMQRGDHKCARELLEENLSV
                     LRELEEEGNTATPLKKFHVLYLLAYMALNEENDPARAAALWEEDLTLARQIGDVLRIG
                     MTMCSLGYAALLQGDYERARALCEETLAFAHELGSAVVEIIPETLVNLGLASLGLGDH
                     ERASASFEEALTMSKSAGRKPTIINILEAMASLAGALGEASRTARLWGAAEAARELTD
                     IALPPGDRALHEPYLADARLRLGEAAWEEALSEGRAMTTDQATDYAFSKEEADPTTIP
                     VPQKPLVADELTPREREIAILVARGLTNRQVSTRLGISERTAGNHVARILRKLGLSSR
                     AQIATWATELHLLTPHPD"
     gene            4736..5251
                     /locus_tag="VHM69_18545"
     CDS             4736..5251
                     /locus_tag="VHM69_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742446.1"
                     /translation="MRRSIVLALGALLALLVAAPVAFGQVADGSASEQARELSAAWWP
                     WALEKSTAASPLNGSYDGGPRCNGQAANGSAKDVWFLAGTITGEKVERTCTVPAGKEI
                     FFPVFNLLDFNVNGAQTEEELQQEVEGWADDALAHPKRPLFATVDGEPVEMNRLVSPS
                     ALFHYTLQKDA"
     gene            5713..>6285
                     /locus_tag="VHM69_18550"
     CDS             5713..>6285
                     /locus_tag="VHM69_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742447.1"
                     /translation="MRRRILLLLTTIVLTLAMANGTAMAVVVDDGVSLGNGFYIPKEF
                     AGMVNNCSGYYWYCLPTGDVTLKADNTSGVCTWTYNIAWGDGHSSSFVSMPGITESAS
                     HKYINPGIYTIDVTIPAGTSSNPSIWCGPLNQRFVVEVPNYRPDTFISTPPAPSPGST
                     VNSKDVTFTYASNRNYVTFECSLDGSDFSQC"
BASE COUNT         1282 a         1839 c         2006 g         1158 t
ORIGIN      
        1 atagagatgt tcgactcact acagcaggga ctaagcgatt tcttcgcctt catcccgcag
       61 ctaatcggcg cgatagtcat cctgatcgtc ggctacatcg tggccaagat ccttcagtcc
      121 gtcctggcga gggtcctcaa ggcggtaggc ttcgacggat ggatggaacg cagcggcata
      181 aagcagttct tcgatagagc ccagacgaac cagacacctg cgactatcct cggcaagctg
      241 gttttctggt tcgtcttcat catcgcgatc acgatgtcca cggatgccct tggcatcgag
      301 caggtatcag cggtcttttc ccagctcatc gcctacatcc cgaatatcat tgctgccgtc
      361 ctgatcctgg tattggcagg actcctggca cagttcgtcg ccgggctggt gcgcggggca
      421 accggcatca acatccttgg caccatcgcc caggccgcaa tcatggtgta cgcggtcttc
      481 gccgcgttga cgcagcttca gatcgcggtc gagcttacgg cgcccacgtt cctgatcgtg
      541 ctcggggccg tggccctggc ggcagcgata gctttcggct ggggcgcccg caacgttgcc
      601 caggacatcg tcgagagcgc atacaaccgc agggacgaag ccaggcagaa ggtacaggag
      661 cagggcgact cctccgcgac caccggcgcg accggcgatg gcgtactcga agacgaggac
      721 agactcgccg tcaggcccct gcgccgagaa gagtagaacc ccgaaaaccg gggcgttcga
      781 cggcgggact cttcggagtc ccgcctcttt cgcgatgcgt gttcccggca ggcattattt
      841 acgacgggct ccgcctgctc cagtggagca cactgtctat cggaagcatc caaccagcag
      901 tggaaagtgg gctcggggcg atcccggagt ccatccttcg ccggcatgtt tcttcccctt
      961 ccaccacgat cgaagcccct gaacggtgat tccggctaga tatcctgatg gagcatctcc
     1021 atccatggga gggtatacgg tttccgtagc ctcgtgatga cgtgcacggt tctagcccag
     1081 tagctatgat gtgctggtta catggaggag atcggctccg ctactctcct caaggagtga
     1141 aacggagccg gtcggggcga tgatgcatct gtcgagatgg ggatggcacg acaccataga
     1201 gtactggcag aggtggagca caagttttca gaaacaacca tcagagaacc cgaggccgta
     1261 agaatcgggt tgctcggcgg cttccgcgtc tctgtgggga cccggactat cgaggagggc
     1321 gcgtggcgcc tgaggaaggc ggcgagcctt gtgaaggtac tcgccctcgc gcggggtcac
     1381 cgcctccacc gcgagcgggt gatggatctc ctctggccgc agcttggcgt gaaggcggcc
     1441 tccaacaatc tacgaggggc actccacgtc gtccgccgca cactcgcgtc cgacacagct
     1501 tcccgctacc tggagcttca gggagatcaa ctggttctgt gtcctagggg gcagttatgg
     1561 gtggatgtgg acgtgtttga ggagaccgct gccaccgccc ggcgctcccg ggatccggca
     1621 gcataccgtg cggccatcga tctgtatgcg ggcgaactgt tgccgggaga ccgctacgag
     1681 gagtggacgg aacgcaggcg ggaagaactc aggcagttgc acctcgcgtt gctcaccgag
     1741 atggccggcc tctacgaggc gcgtgagcaa tacgagagag gcatcgaagc cctcaggcgg
     1801 gtactctccg aagagccggc aagagaagag gcgcatacct ctctgatgcg cctctacgtc
     1861 ctctcgggca agcgccagga gtcgattctc cagtacgagc ggctccgtac atcactcacc
     1921 agggagctcg acgttgagcc cggtatcgaa agcagacaac actacgaaga gataagagcg
     1981 ggggaatctc tggcgactgg ctccgccggt gatccttcca agagagccgc ggaccccgct
     2041 cctcacaacc tgcccgcctc cgtgaccagc ttcgtcggcc gggagcgcga gatgatcgag
     2101 gccaggcgtc tgctctcgat gacgcggctc ctgaccctcg caggcgccgg cggctcgggc
     2161 aagacgcggc tcgccctgga aatatccagg gatctcgcgg gtctctacca ggacggggtg
     2221 tggttcgtag agctggcacc gctctccggg ccggatcttg tagcccagga ggtggcaggg
     2281 gctctggagg tacaggagcg tcccggccaa ccgcttacag gtacgctcgt cgacagtctg
     2341 cgtgacagag agatgctgct ggtactcgac aactgcgagc acctgatcga tgccgccgcg
     2401 cgcctcgccg acgagctcct ttactcgtgc ccgcgcctga gggtgcttgc cacaagccgc
     2461 gagaccctgg gtatccaggg agaggtcgta tggcaggtga agccgctctc cctgccagaa
     2521 gcggatggag gtgccagcgt cgagggcctc atgctctacg aggcggtcag gctattcgtg
     2581 gatcgcgcgc gcctgaggct gcccgacttc gagttgatgg aggcgaacgc cggcgcggtg
     2641 tcgagggtgt gtaggaaact cgacgggatg ccgctcgcca tagagctcgc cacggcgcgt
     2701 atgggggcgc tcgtggtgga tcaggtcgcc cagaggctcg aaggctccct cgacgtgctg
     2761 agcgccggca gcagaaccgc agaaccgcgc cagcagaccc tgagggcgac catagattgg
     2821 agccacgagt tgctcagtca gaccgagcgg gctctgttca ggaggctctc gatctttact
     2881 ggagggtgga ccctggaggc ggcggaggat gtatgctcgg gagagagcat ctacgaaggc
     2941 gatgtcctgg atcttctggg cagtctggtg aacaagtcgc tcgtggtcgt cgctgccacg
     3001 ggcagcgcgg cacgttacag gatgctcgag cccatccgcc agtacgccag ggagaagctg
     3061 gaggagagcg gagaaatcga cgagatgcgg agcagacacg ccgccttctt cctcgctctg
     3121 tccgaacaag ccgagccgga gctgtccggc actcagcaga ggatgtgggt agagcaactg
     3181 gagggggagc acgacaatct gcgggaggca gtctcgtggg ttctcgaaag agagggagcg
     3241 gagtcagccc tgcggttcgg cggcgcgctc tggcggttct ggtttgctca agggcacctg
     3301 agtgagggac gcaagtggat ggaacagata ctcgcgaagg gtaaaccatt atccacgctg
     3361 gcacgggtca aggcgctcga gggtctggga tggctggcgc agtccctggg tgatcccggc
     3421 ctcgtcgaag cgacgttcga agagatgctc agtctatctc gaaagctagg cgacaagggg
     3481 aacatcgcga ccgccttgaa cgccctaagt ggccaggcta tgcagcgggg tgaccacaag
     3541 tgcgcgaggg agttgctcga ggagaacctt tcggtgctgc gggagttgga agaggaagga
     3601 aacaccgcca ccccgctaaa gaagttccac gtactctacc tgcttgccta catggcgctc
     3661 aacgaggaga acgatcccgc acgggcggca gcgttgtggg aggaggatct gacgttggcc
     3721 aggcaaatcg gcgacgtctt gcgcatcggt atgaccatgt gcagcctggg gtacgcggca
     3781 ctgctgcagg gcgactacga gcgggcgagg gcgctctgcg aagagaccct ggcgttcgcc
     3841 cacgaactcg gtagcgcggt cgtagagatc atccccgaaa ccttggtcaa cctggggctt
     3901 gcgtcgctcg gtctaggaga tcacgagcgt gcgagtgcgt ccttcgaaga ggccctgacg
     3961 atgagcaaga gcgcagggag aaagcctaca ataatcaaca tcctggaggc gatggcaagc
     4021 ctggcaggag cgctggggga agctagccgg actgcgcgac tgtggggggc tgcggaggcg
     4081 gctcgcgaac tcacggacat cgcattgcca cctggcgacc gggcgctgca cgagccctac
     4141 ctggctgatg cgcgtttgcg gctgggggag gcggcgtggg aagaagcgct gtccgaggga
     4201 agggcgatga caacggatca ggccaccgat tacgcgttct cgaaggagga agcggatccg
     4261 accacgattc ccgtgcctca aaagccattg gtggcggacg aactcacccc tcgcgagcgc
     4321 gagatagcca tcctcgtcgc caggggactc accaaccgcc aggtctccac caggctcggg
     4381 atctccgagc gcaccgcagg caaccacgtc gccaggatac ttcgcaagct cgggctcagt
     4441 tcgcgggccc agatcgccac ctgggcaacc gaactccacc tgcttacgcc ccacccagat
     4501 taaagctttc cttcggagcg tccagaaggc aatccattat cttgagtgcg gatcgaagat
     4561 gagtagaaat actcattccc tttcccctcc cagctaacgg ctggctaacg ccctctctct
     4621 aagctgcacg gcgtccacta gagagaggag atgccgtaga gagatagagt aaggtcgggt
     4681 ttgtcgtagg tagcgacttc tctgtagagg ttgccacaag ggaatggagg attcagtgag
     4741 acgaagcata gtgttggccc tcggcgctct tctggcgctg ctcgtggccg cgccggtggc
     4801 cttcggacag gtcgcggatg gttcggcatc ggagcaagca agagaactgt ccgcggcgtg
     4861 gtggccatgg gcgttggaga agtcaacggc tgcaagccct ctgaacggca gttatgacgg
     4921 tggcccccgc tgtaacgggc aggccgcaaa cggtagcgcg aaggatgtat ggttcctcgc
     4981 cggtacgatc acaggcgaga aggtggaacg aacctgcacc gtgccggctg gcaaggagat
     5041 cttcttcccc gtgttcaatc tcctcgactt caacgtcaat ggggcccaga cggaagagga
     5101 actccaacag gaggtcgaag gctgggccga cgatgcgctg gcccacccga agcgcccctt
     5161 gtttgccacc gtggacggag agcccgtaga gatgaatcgg cttgtttctc cttccgccct
     5221 cttccactac acccttcaga aggatgctta actgacggac ttcggtgtag agccgggacc
     5281 atacgttggc gtgacggatg gcgtgtgggt gacgctgccg ccattgtccg agggcgagca
     5341 cacgatccac ttcggaggca atgggcagaa caacacgtat aacctcaacg tggaatgaat
     5401 ccacagcgtg tatccacccc cggaacgggg tgaggtccgg gaggctgtcg caaggtggca
     5461 gcctcccttc ttctacattt cgaacgccct tgtagaacga ccacactcca ggcaatcacc
     5521 gcggatgcgg ggagcagcag cctcggccct tctcttatgc tcgcaggagg ttccaaaggc
     5581 ggtcaattct ctcaggtccg aggcgaaaat gagtagaaat actcataccc ttccccttcc
     5641 ggctaacggc cagctaacac actccagata tgctgcgtag cgtcataaca gcggaaagag
     5701 cggggtagcg aaatgaggcg gcgaatccta cttctactga ccacgatagt gctaactttg
     5761 gccatggcga acgggacggc gatggcagtt gtggtggatg acggagtcag tttaggtaac
     5821 ggcttctata tccctaagga atttgcagga atggtcaata actgttccgg ctattactgg
     5881 tattgcctgc cgaccggcga tgtaaccctc aaagcggaca acaccagcgg tgtctgcacc
     5941 tggacctaca atatagcatg gggtgatggc cactcgagtt cgttcgtttc gatgccgggc
     6001 atcaccgaga gcgcatctca caagtacatt aatcctggca tttacaccat agacgtaact
     6061 atccccgctg gcacctcttc gaacccttcc atatggtgtg gacccctcaa ccagcggttc
     6121 gtagttgagg tgcccaatta tcggcctgac actttcatca gcactcctcc ggctccttcc
     6181 cccggaagca cggtgaactc gaaggatgtc accttcacat acgcctctaa caggaactat
     6241 gtcaccttcg agtgcagtct cgatggcagc gatttctcgc agtgc
//
LOCUS       DATAIR010000782         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_269986, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000782 DATAIR010000000
VERSION     DATAIR010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269986"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VHM69_18555"
     CDS             complement(<1..965)
                     /locus_tag="VHM69_18555"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2742448.1"
                     /translation="MNESVHEPEEKTSIADALATTLRQPSFLRVMLVLAATVVVLVGI
                     RLAAPILNPIFFALVLTLLFSPVYGWLKNRGLPSPLALVIMLIFVGAIFAVLFFILGI
                     SISRFSERVGFYTTQLNGQLDSLDALIKRAGLSNVDVRDVVKPSALADALGTVLAGIA
                     GFLSDLFLILMIMLFLLGEGPAMMDRLRAGTSRDNPQVERLTTVGRSVVRQFGLRAIV
                     NLVTGAGVTVMLFALGVDFPLLWGILTFFLSFVPYIGLVLAVTPAVVLALAEFGVSRA
                     VLVIIGVIVINVLAENVLSPLMMGRGLNISPTVVFLSFIVWAWLLG"
     gene            complement(1183..2319)
                     /locus_tag="VHM69_18560"
     CDS             complement(1183..2319)
                     /locus_tag="VHM69_18560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2742449.1"
                     /translation="MPSQDGGWARASTSQVARVVAVALLTAAVVLGAFVLLWQVRTFI
                     GWFVIALFLAAVLNPAVNWLQQRHRLIKRPLAIGLTYLGVLVALLVVVGIFVPVLVDQ
                     INGFIKFVTTAANAPEGPTEYIKGLAKDNGLGGLFQRFSDQLDELRKQLGEVLQNLLS
                     ASGQIAIGLAGALAALATVLTLTFFLILGSERYVNAGVGLFPERHQPLVRRLLSQSAG
                     AISGYITGNLAISVICGVTTFVVLILLGMPYAAPLALLVAVLDLIPLVGATLGGVLLV
                     LVGLFVEPWKAVVLLVFVLVYQQVESNFLQPIVYSKAVQLNGLVILIALLVGGQLLGI
                     PGALLAIPVAEIIRIVVTELLDYRRRGQEEAREPAAAAPTTNPD"
     gene            complement(2596..3084)
                     /locus_tag="VHM69_18565"
     CDS             complement(2596..3084)
                     /locus_tag="VHM69_18565"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2742450.1"
                     /translation="MATLSVLKFNDPYGADRVLLALQGLQEQQMITLEDAAVVSWPQG
                     NKKPTTRQLHSTSGMGAMGGAFWGFLFGLIFFVPFLGAAIGAGMGALTGSLADVGISD
                     DFIKQVREKVTDGTSALFALTSGATAPDKVVEELKAYDFEIISTNLPKEQEDKLREAF
                     AQ"
     gene            complement(3294..3785)
                     /locus_tag="VHM69_18570"
     CDS             complement(3294..3785)
                     /locus_tag="VHM69_18570"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2742451.1"
                     /translation="MSDNPMFLYAGEYESVDDAKADLDELKRLHDEQFVGTYDAAVLT
                     KEDGKVKIVDKIEKPTQHGGWAGLAVGAALGLIFPPSVLVSGLVGAGAGAVIGHLRGG
                     MSRSDLKEIGEMLEESESALIVVGEATIERGVDEAIKHAKKQMKKEVRADAREIERAI
                     DSV"
     gene            4397..4630
                     /locus_tag="VHM69_18575"
     CDS             4397..4630
                     /locus_tag="VHM69_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742452.1"
                     /translation="MRTKTVYRIVVRDELSERYAMAFEGMEMKIKNGQTILTGEIIDQ
                     PHLHGILGLINGFGLKLISVESLTDELEDDATN"
     gene            5066..5476
                     /locus_tag="VHM69_18580"
     CDS             5066..5476
                     /locus_tag="VHM69_18580"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2742453.1"
                     /translation="MHRETFETATRGTGARRVQTGGIARYRPQVVVSLLVVIVLVGGA
                     LYTARLSEGAPRVVYTASLEQIQDESQDPLKIDINTADAEQLDELPEVGPSTAESIVE
                     YRRLNGEFRFVDELEEIPGIGPKKLEKIEPFATV"
BASE COUNT         1131 a         1700 c         1624 g         1107 t
ORIGIN      
        1 cctagcagcc acgcccagac gataaaggac aggaagacga cagtcggcga gatgttgagc
       61 ccacgtccca tcatcagggg tgaaagcacg ttctcggcga gcacgttgat cactattacc
      121 ccgatgatta ccagcacagc ccggctcacg ccgaactcgg ccagtgccaa aaccacagcg
      181 ggcgtcaccg caagtaccag cccgatgtat ggcacgaagc tcaggaagaa tgtgaggatc
      241 ccccacaaga gcgggaagtc caccccaagc gcgaagagca tgacggttac gccggcgccc
      301 gtgacgaggt tgacgatggc gcgcagcccg aactgccgca cgacgctccg accgacggtg
      361 gtcaggcgtt cgacctgcgg gttatccctg ctagtgcccg cgcgcaaccg atccatcata
      421 gccggtccct ctcctagcag gaacagcatg atcatcagga tcagaaacag gtcgctcagg
      481 aaccctgcga tgccggcgag tacggtgccc agggcatcgg cgagggcgct tggcttgacc
      541 acgtcgcgaa cgtcgacgtt cgagagcccc gcgcgcttta tcagggcatc gagactatcc
      601 aactgtccat tcagctgtgt cgtgtagaac ccgacgcgct cgctgaacct gctgatagaa
      661 ataccaagaa tgaagaagag taccgcgaag atggcaccaa caaagatcag catgatcacg
      721 agggcaagag gcgaaggaag tccgcgattc ttgagccagc cgtagacagg gctgaacagg
      781 agggtcagaa ccagtgcgaa gaagataggg ttgaggatcg gcgcggccag acgtatcccg
      841 acgagcacga ctaccgtcgc ggcgaggacc agcatcaccc gtaagaacga gggctggcgc
      901 agcgtggtcg caagcgcatc ggcgatactg gttttctctt ccggctcgtg cacggattcg
      961 ttcatgatct aacctccgag ttgcgcttgg ccgcttgccg agcatggttc acagccttct
     1021 cagatcgttt tgcgcggctc cttcctgctg tcccagagga tcagtgaacg acgactattc
     1081 acggtcttcg ccttggcaag gccactggaa gaacgctcca tacatggata tcttcttggt
     1141 acgcaatcct cagttcctct ccttgacttc taacctgcta cctcagtcag gatttgttgt
     1201 cggcgccgca gcggcgggct ctctcgcctc ctcctgtccc ctccgccggt aatccagcaa
     1261 ttcggtgact actatgcgaa tgatctcggc taccgggatg gccagcagcg cgccggggat
     1321 accgagcaac tgtccgccta ccagcagggc tatgaggatc accagcccat tgagctggac
     1381 ggctttgctg tagactatcg gctggaggaa attgctctcg acctgttgat agaccaacac
     1441 gaaaaccagc aagaccacgg ccttccacgg ctcgacgaac aaccctacga ggacgagcag
     1501 cacaccaccc agcgtcgcac cgaccagcgg tatgaggtcc agcaccgcaa ccaggagcgc
     1561 tagcggggcg gcgtagggca tgccgagtag aatgagcacg acgaaggtgg tcactccgca
     1621 gatcacgctg atggcgaggt tgcccgtgat gtagccgctg atcgccccgg ccgactggga
     1681 gaggaggcgt cgcacgaggg gctggtgtct ttctgggaag agcccaaccc cggcgttcac
     1741 gtaccgctcg ctgccaagga tcaggaagaa ggtgagcgta aggacggtag ccagcgcggc
     1801 gagcgcaccg gcgaggccaa tggctatttg cccgctggcg gacaggaggt tctggagcac
     1861 ctctcccagt tgtttcctga gctcgtcgag ttggtcgctg aacctctgga agagcccacc
     1921 tagcccgtta tccttggcaa gccccttgat gtattctgtt ggaccctcgg gggcgttggc
     1981 cgcggtggtg acgaatttga tgaagccgtt tatctggtcg accagcacgg gcacgaagat
     2041 ccccaccacc acaagcagcg ccaccaagac tcccaggtag gtgagcccta tggctaaggg
     2101 acgtttgatc aacctgtggc gctgctgcag ccagttgacc gccgggttga gcaccgccgc
     2161 cagaaagagg gcgatcacga accagcctat gaaggtcctg acctgccaga gcaagacgaa
     2221 agcgccaagc accaccgccg cggtcagtag ggcgacggcg accacgcgcg ctacttgcga
     2281 agtcgaggcg cgtgcccacc cgccatcctg gctcggcacg ggcgctcctg gctgcgcaga
     2341 tgtgatcggg ggctgtgcct gggcatttgc ctgcgagtcc ctatgtgaag cctttccctc
     2401 cgtcatatgt gtagctccta ctgttctcca ggctgcgact cgtcggctgt gtcgggtcga
     2461 ttgccagtcg aagtcctcat gtgccggact ccttccatct ggcctatcgg tgtggccttt
     2521 tgggagggtg gtcctcacct gcccgccttg gagcggctaa cccgctgacc gctccaaggc
     2581 gatctctact tactgctatt gggcgaaggc ctcgcgcaat ttgtcctcct gctccttggg
     2641 caggttggtc gagatgatct cgaagtcgta ggccttgagc tcctcgacga ccttgtccgg
     2701 ggccgtcgcc ccactggtca aggcaaacag agcggatgtc ccgtctgtga ccttctccct
     2761 tacctgcttg atgaagtcgt cgctgattcc cacatctgcc aaagagccag ttagtgcacc
     2821 catcccagcg cctatggcag ctccgaggaa gggcacgaag aagatcagcc cgaacaaaaa
     2881 gccccagaag gcaccaccca tcgctcccat gccggaggtg ctgtgtagct ggcgagtggt
     2941 gggcttcttg ttgccctgtg gccagctgac gacggccgca tcctcaaggg tgatcatctg
     3001 ctgctcttgc aggccctgca gggcgaggag caccctgtct gccccgtagg ggtcgttgaa
     3061 cttcagtacc gatagcgtag ccactggcta atctcctttc agttctgggt gcacgaatgc
     3121 gatgcatctc gagagcccac tgaggagggg ccctcgctcc gatatttcct ccggagatct
     3181 cgctccactc ctgagcctgg aggcttaccg tggtcatgcc agtatcctcg gcgccgacgc
     3241 gaaattgcgc ctgacggcgg tggcgcgagc cctgctcatc tctcattggg ggactacaca
     3301 gagtcgatcg ccctctcgat ctccctggcg tctgccctta cctccttctt catctgtttc
     3361 ttggcgtgct tgatcgcctc atccacgcca cgctcgatag tcgcttctcc aaccacgatt
     3421 aaggccgact cgctctcttc gagcatctcg ccgatctcct tgaggtcaga tcgcgacata
     3481 ccgccccgga gatgtccgat gaccgccccg gcccctgcgc ccacaagccc gctcaccagc
     3541 actgacggag gaaagatgag accgagcgcc gcgccgacgg ccagcccagc ccaaccgcca
     3601 tgctgggtcg gtttctcaat cttgtctact atcttgactt tcccatcctc cttggtgagt
     3661 accgcggcat catatgtgcc gacgaactgc tcgtcgtgga gtcgcttgag ctcgtcgagg
     3721 tctgcctttg cgtcatcgac gctctcgtac tcgcctgcgt agaggaacat cgggttatcg
     3781 ctcacccaat ttctcctttc tgtcctatat ctatgcccag aagagttacc cgaccaaagc
     3841 cccctttcgt cttctctcgg ctccgtagtc gtccgctgcg aacttcgatc gctctcgccg
     3901 aggcgcgctc tcctctcctg caccactcct cgtgtacgac actctaatct cgttgcaaga
     3961 cggctttcat cacccagcgg gacgtttccc ctaggggatt tcttgaggat gacggcgcat
     4021 gtcgcgtcta gcccaccgaa cgtagggtgc caggaaacac cagctgcctt gaggagggac
     4081 ccgcaagccg atgggacatg cttcagatct atcccgacgc aggcatcttc ccagacctcc
     4141 tggagcgcca acagcgcagg gtgggatcgc tcgacgctga tctcacggtg cgcgagcccg
     4201 ctgtgcttac cggaagttcg gagtatcctc gcgcaaggga gctgtggtgg aggcgcttgc
     4261 cagagggctc atctagcatc aacgacgaac ctgttaatcc cctaggggaa atacgccgat
     4321 gggtgaagat aatccttcct gctctgcatt acgctcagag aacgatgtgc agagcggtga
     4381 cgatcagggg acccgcatga gaacgaaaac tgtctatcgg atcgtggttc gtgacgagct
     4441 cagtgagcga tacgctatgg cattcgaagg gatggagatg aagatcaaga acgggcagac
     4501 cattcttacg ggggagatca tcgatcagcc tcacctccat ggcattctcg gtctcataaa
     4561 cggttttgga ctcaagctaa tcagcgtcga gtccttgacc gacgaactag aggacgacgc
     4621 gacgaactga tcgccagcgg tcgatcgcca ggataggagc actgtcgtag agagatagcg
     4681 atcgtggaga tcgacggtac tccactgggc cggtatcccg caaatgtagt atttgctgct
     4741 ttcgtgatgt caatggaacg ctcacgtttc gtcggcgaac tatcctcacc tgaagtccgg
     4801 gatctagcca gcccgcggca ccccttcgta aaagccggcg cgtgtagttg gcaccggacg
     4861 tctctaatca cacgccaagt gtggcaatcg gcctcctcgc aggagatctt gccccacagg
     4921 gggcgtaggc agacttccgt cgattcctac cgccaacggg cgcggtggaa cgctcggagg
     4981 aaatgtacgt gaacgaccag ccgggttcgt agctcttcgt atctctacta aaggttttca
     5041 tgccgcctgc cgatgacctt tcgtgatgca cagggaaact ttcgagacgg ccacgcgtgg
     5101 tactggtgcg cggcgggtgc agaccggcgg cattgcgcgt tacaggccgc aggttgtcgt
     5161 ctcgctactg gtggtgatcg tgctcgtcgg cggcgcgctt tacacggcac gtctctcgga
     5221 aggggcgccc agggtcgtat atacagcttc actggagcag atacaggatg agtcgcagga
     5281 cccactcaag atagacataa acaccgcgga tgcagagcag ctcgacgagt tgcccgaggt
     5341 cgggccatcg accgccgaga gcatcgtcga gtacaggcgg ttgaatggag agtttagatt
     5401 tgtggacgaa cttgaggaga taccgggaat cgggccgaag aaattagaga agatagaacc
     5461 cttcgctacg gtctaaggag gcatcgtggg cctgacctgg atctcagccc tcccgtcagg
     5521 ctggacctgt gggctattct gctcgccatc agtatcgcgg tc
//
LOCUS       DATAIR010000783         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_270655, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000783 DATAIR010000000
VERSION     DATAIR010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270655"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..2179)
                     /gene="asnB"
                     /locus_tag="VHM69_18585"
     CDS             complement(296..2179)
                     /gene="asnB"
                     /locus_tag="VHM69_18585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX2742454.1"
                     /translation="MCGIVAIFSPERGVSVDALQAATVRLRHRGPDGRRTWVSAEGQA
                     GLGHTQLNIVSPAGVQPIASEDGQRWIVANGEFYDFVAIRNRLEARGHRFRTQTDSEI
                     ALHLYEERGASCLEELRGEFAFVVWDGASKTLFAARDRFGIKPLFYTRLGEAWILASE
                     VKALFAAGLDATWNHEAVYQQIFGCFRPDRSLFAGVRQVPPGHYLQIGQGSHRLTSYW
                     DVNYPRRRRTRDFREAECVQEVRRLLAEAVTLRTQAHVPVGYLLSGGLDSSTVMSMAA
                     AVSDRPVQAFTIAFDGTPYDESLKARAAAEHVGARLEILRVTDADVADNFAGSVWHGE
                     TIQINAHGTARFLLSREIQASGYRAVMAGEGADELFAGYDFVRSAVAKRSALRLVPRL
                     LKMLGALNQAERRIALASPLLARICRALPQAFVSPFADASGLLHSLLSLDFVARFAGH
                     DPYRDLFRHLDWMRQMWGRERAKQVMYVWLRSIFVNYHMAAERLDMAHAVEVRLPFLD
                     HELFDYASQIPVSLLAGEGRQKHLLREAARPFVAEATYVGAKQPFYAPPTALRPDSRL
                     HELVQDTVRGETMASLPFFNQKAALALLDGAPRLEPGARTVLDPLLMVIVSLCLLQEQ
                     FAL"
     gene            complement(2189..4015)
                     /locus_tag="VHM69_18590"
     CDS             complement(2189..4015)
                     /locus_tag="VHM69_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742455.1"
                     /translation="MTEARAFYRWGREMTWRGLSTALAGFRSTLRHLPSGSAGLPEVD
                     LDPVLRALRTAVSDGEKRVRGSMWMERLPEALAELRMSGTAGVVAGLNRESGSFTGSL
                     ARTRLGRAWRLFTGPTDALPIDLFRVLVGLLGCAYFSRTLFEAPTFSGPNGLIDHGLV
                     RRTLWYTRLGFFPPGAGLNFFRVVYALAAMGSLLLAAGIWVKPVSAFLYATAVSTYRR
                     NFPVTYVDDALMHLALLWMLLLPIGNTLTLPEWHSERGAAVERWKKVRVQGAAVRCLM
                     ANLALVYIVAGLWKWTSPLWKEGLAVYAILKTAVSRTPDLWRPGHVPALRAANHTALV
                     LEPLFPVAFVLPSKHPLKWLLGAAMFGFHAGIIATLKIPFANIACIAALVLVFREEIM
                     AWLRGDSEDSPRSESPAGEFGLAERAACLLVAWLALAMLSEATRPYWRYGTPPVVKEN
                     RELANRTPGMGQNPFYGVLWLVGLAQSYRLFDWVDEYNYHIAYEVVERQRGGAARTLD
                     DGALFPRTIRNVLLQSYLQDAVWMKVPRRHLPDLQRSVFERYVRRFCSRCQHDGRIEV
                     VAKVQRTTAANLGLDRPRRVRFLEFTCGGREPRLHFMRLARS"
     gene            complement(4216..4548)
                     /locus_tag="VHM69_18595"
     CDS             complement(4216..4548)
                     /locus_tag="VHM69_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2742456.1"
                     /translation="MPETDLIDTQELKAKLDRGDDFKVVMTLGEWEYRTTHIPGSVRI
                     STVEEALTALDPEDEIVLYDSGPTCTASRMALRILKTHGYARVRRYPGGLEEWARAGY
                     PLTGENAG"
     gene            complement(5096..>6067)
                     /locus_tag="VHM69_18600"
     CDS             complement(5096..>6067)
                     /locus_tag="VHM69_18600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742457.1"
                     /translation="MAALGTLAAGLAHELNNPAAAIRRSAAQLRDALAERERLAAQLS
                     SIAGEPLQNDTFGTLQAEAMKHTTVAPLYDPLTLSEQEDELQEWLEDHGVGQAWELAP
                     VLVSFGWDRVELERLADSFSPKQLPFVVRWLGANSSVYGLLDEVGHSAEAMSEIVKAV
                     KTYSYLDQAPIQEVDVIESLENTLVLLRPKINADISITRDYADDVPRIEAYGSELNQV
                     WTNLIDNAIDAIQGQGELTLHVHSEGDAVTVEIIDSGPGIPREIRQRVFEPFFTTKPP
                     GAGTGLGLHIAYNIVVHKHHGQIQVASRPGETRFGVELPVRLASSNT"
BASE COUNT         1166 a         2005 c         1799 g         1097 t
ORIGIN      
        1 aacgcccaca cgctgatggc caccatcaag aacgccagcg actccgcccc gaggacatcc
       61 ctgcgacgcc aggcgtagaa cgcaagcacg cccgagatgg cggctgctat gagcaggctg
      121 agcgtatatg cgaactggtt catcatgccg ccagccacgc tatcaaccac tcccaaccac
      181 tgatggtccg acgtagatcc accacgaatc aaattataga ggctcacaga ggttgcaatc
      241 attgtttacc agcgatacca gatcaatccc gcaaagggaa ggtgggtttt tcccgctaaa
      301 gggcgaactg ctcctggagc agacacaggg acactatcac catcaagagt ggatccagca
      361 ctgtcctggc gcctggttcg agcctcggcg cgccgtctag cagagccaac gccgccttct
      421 ggttgaagaa gggcagcgac gccatggtct cgccgcgcac ggtgtcctgg acgagctcgt
      481 gcagccggct gtccggacgc agggcggttg gcggcgcgta gaagggttgt ttggctccaa
      541 cgtatgtagc ctcggcgacg aacggacggg ccgcctcgcg cagcaagtgc ttctggcgtc
      601 cctccccggc gagcagcgac acggggatct ggctcgcgta atcgaacagc tcgtggtcca
      661 ggaagggaag gcgcacctcg accgcgtggg ccatgtccag tcgctccgca gccatatggt
      721 agttgacgaa gatggagcgc agccacacgt acatgacctg ctttgcccgc tcccttcccc
      781 acatctgacg catccagtcg aggtgtcgga aaaggtcccg gtacggatcg tggcccgcga
      841 acctggcgac gaaatcgagg gagagcaagg agtgcagcag ccccgacgca tcagcgaagg
      901 ggctcacgaa agcctgcggg agagccctgc agatccgcgc gagcagcggc gacgcaaggg
      961 ctatccggcg ctctgcctgg ttcagcgctc cgagcatctt gagcaacctg gggaccaggc
     1021 gcaacgcgct tctctttgcc accgccgacc gcacgaaatc gtacccggcg aagagctcgt
     1081 cagcaccttc gcccgccatc accgccctgt agcctgaagc ctggatctcc cggctcagca
     1141 ggaatcgggc tgttccgtgg gcgttgatct gtatggtctc tccgtgccag acggagccgg
     1201 cgaagttgtc agccacgtcc gcatctgtga cgcgcaggat ctcgaggcgc gccccgacgt
     1261 gctccgcggc ggcgcgcgcc ttgaggctct catcgtacgg tgtcccatcg aaagcgatgg
     1321 tgaacgcctg cacaggccgg tcgctgacgg ccgccgccat gctcatcacg gtggatgagt
     1381 ccagcccgcc actcaacaag tagcccacag gcacgtgcgc ctgcgttctc agcgttaccg
     1441 cctccgccag cagccggcgc acctcctgca cgcactcggc ttcccggaag tcgcgcgtcc
     1501 gtctgcgccg agggtagttc acgtcccagt aggaggtaag acggtgggat ccctgcccta
     1561 tttgcaaata gtgacccggc gggacctgtc tcaccccggc gaaaagcgag cgatccggcc
     1621 taaagcagcc gaagatctgc tggtagacgg cttcatggtt ccaggtggca tcgaggcccg
     1681 cggcgaagag tgccttgacc tcggaagcca ggatccaggc ttcccccagc cgcgtgtaga
     1741 agagcggctt gatgccgaag cggtcgcggg cggcgaacag cgtctttgaa gcaccatccc
     1801 agaccacgaa ggcaaactcc ccgcgcagct cttccaggca cgaagccccc cgctcctcgt
     1861 agaggtggag ggcgatctca ctgtccgtct gggtgcggaa gcggtgacct ctggcctcaa
     1921 gcctgttccg gatggccacg aagtcgtaga actcgccgtt ggccacgatc cagcgttgac
     1981 catcttcgct ggcgatgggc tgcacaccgg cgggcgaaac gatgttgagc tgggtatgcc
     2041 cgagacccgc ctggccctcc gctgagaccc atgtgcgccg tccatccggt cctcggtggc
     2101 gcaaccgcac agtcgccgct tgcaacgcgt ccactgagac gccccgctcc ggcgagaaga
     2161 tggccacgat gccgcacatc gtcctgcctc agctcctcgc cagacgcatg aagtggaggc
     2221 gtggctctct ccctccacac gtgaactcca ggaagcgcac gcgtcggggc cggtccagac
     2281 ccagatttgc agcagtcgtc cgctgcactt tggcgaccac ctctatgcgg ccgtcatgct
     2341 ggcacctgct gcagaagcga cggacgtatc tctcgaacac ggaacgctgg agatcgggca
     2401 gatgccggcg cggcactttc atccatacgg catcttgcag gtaggattgc aacagtacgt
     2461 tccgaatggt gcgcgggaag agggccccgt catccaacgt gcgggccgca ccacctctct
     2521 gtcgctccac cacctcgtac gcgatgtggt agttgtactc gtcgacccag tcgaagagcc
     2581 ggtagctctg cgcgaggcca acgagccaga gtactccgta aaacgggttc tgacccatgc
     2641 ccggcgtccg attcgcaagc tcccggttct ccttcacaac gggaggcgtg ccgtaccgcc
     2701 agtacggacg cgtggcctct gaaagcatcg ccagagcaag ccacgccaca agcaaacacg
     2761 ccgccctctc ggcgaggcca aactccccgg cggggctctc gcttcggggc gagtcctccg
     2821 aatctcccct cagccaggcc atgatctcct ctcggaagac caggaccagc gccgcgatgc
     2881 aggcgatgtt ggcgaagggg atcttcaagg tcgcgatgat gccggcgtgg aacccgaaca
     2941 tcgccgcccc gagcaaccac ttgagtgggt gtttggaggg cagaacgaag gcaaccggga
     3001 agagcggctc gagcacgagt gccgtgtgat tggccgcgcg gagcgcgggc acatgacccg
     3061 gccgccagag atccggcgtc cgggagacgg ccgtcttgag gatcgcatag accgcgagcc
     3121 cctctttcca caggggactt gtccacttcc acagacccgc cacgatgtag accagcgcca
     3181 ggttggccat aagacagcgc accgccgctc cctggactcg caccttcttc caccgctcca
     3241 cagccgctcc ccgctcggag tgccactcag gcaacgtaag ggtattcccg atgggcaaga
     3301 gcaacatcca caacagggcc aggtgcatca gggcatcgtc cacgtaggtg acagggaagt
     3361 ttcgacggta ggtggaaacc gccgtagcat agaggaaggc gctgaccggc ttgacccaaa
     3421 tgcccgcggc aagtagcagg gagcccatcg ccgccaacgc gtacacgacc cgaaagaagt
     3481 tcaatccagc cccagggggg aagaaaccca gtctggtgta ccagagggtc ctgcgcacga
     3541 gcccgtggtc gataagcccg ttcggaccgc tgaacgtcgg tgcttcgaag agtgtgcgcg
     3601 agaaatacgc gcaacctagc agcccgacga ggacgcggaa gaggtcgatc ggcagcgcat
     3661 ccgtgggccc tgtgaacaac ctccacgccc gtcctaaccg ggttcttgca agtgagccgg
     3721 tgaaagaccc tgattctctg tttagcccag cgactacacc ggccgttcca gacattcgca
     3781 attcggctag ggcctcaggc aaccgctcca tccacatgct gcccctgacc cgtttctctc
     3841 cgtcggagac ggccgtccgc aacgcccgca ggaccggatc taggtccacc tccggtagcc
     3901 cggcgctccc gctaggcaga tgccgcaggg tggagcgaaa tccagccagg gccgtggata
     3961 gccctctcca cgtcatctcg cgtccccagc gatagaacgc gcgtgcctcc gtcatcagga
     4021 attcaccccc tctctacctc aaccccagac gccgcgaatc agcatcccgt cctctttacc
     4081 taccaagtcg ctcgtcctcc ccatcctttc cttacagcgt aagtatacaa tatagtgctt
     4141 acgctgaata ttgtcaaggc gtgcgaggcg tttcgaggaa tccgccagcc cagggtaccg
     4201 aagcgtttac ttcggctatc cggcgttttc accagtaagc ggatagcctg cgcgtgccca
     4261 ttcctccagc ccaccgggat agcggcgcac tcgtgcgtac ccgtgggtct tcagtatcct
     4321 caaggccatc cgactcgcgg tgcaggtggg accagaatcg tagaggacta tctcgtcttc
     4381 ggggtcgagc gccgtgagcg cttcctcaac tgtgcttatg cgcaccgagc cggggatgtg
     4441 cgtagtgcga tattcccact cgcccagggt catgacgacc ttgaagtcgt cccccctgtc
     4501 gagcttcgct ttcaactcct gggtgtcgat gaggtccgtt tcgggcattc gggaaactcc
     4561 ttctaatcag tgatcctacg agcaggcgtg agcccattcg gaggtcgggc gcggcctccc
     4621 aaaggagcgt ctgctctgcg gctcccactg gcctcttgcc ccttagcacg agcgcggcgc
     4681 tgaaggcgct ggtccaggtg cccatggcct tgccgcgccg ctcctcctcg aaagaggcgc
     4741 cgatgatcgc caatgagttc ggaacttgga gggccgcgcc tactccctgc acctccttcg
     4801 agcggtttac ctgctcgtcg caaggctgtc ttataacgtt ggccaaaggt ccctctgtcc
     4861 gggctactgg agccggtgct ccgtccgcca cggtgtcttc catgcgcgcc atgttcctgg
     4921 ccggtggcac gtagatgtcc cgcgacctta cgcagcttca tctgccacgc ggcgatctaa
     4981 cattaccttc cctgacacgg caggcaaatc ccccaaatgg cgtattttgc agattctctt
     5041 caatggcggc tgatgaataa gggatcggtc ggcggcctct gatcccgcag gttgctcagg
     5101 tgttactcga agcgagacga accggcaact ctactccaaa gcgcgtctcg ccgggtcttg
     5161 aagcgacctg aatctgccca tggtgcttgt ggaccactat gttgtaggcg atatgcagac
     5221 ccaggccggt gcccgcgccg ggcggcttcg tcgtgaagaa cggttcaaaa acgcgctgcc
     5281 ggatctcacg cgggatcccc ggtccgctgt cgataatttc gaccgtgacg gcatcgcctt
     5341 cagagtggac gtgtagcgta agctctccct gaccctgtat ggcgtcgatc gcgttgtcga
     5401 taaggttggt ccacacctgg ttgagctctg atccgtaggc ctcaatgcgc ggcacatcgt
     5461 cagcataatc tcgagtgatg ctgatgtcag cgttgatctt gggtcgcagg agcaccagtg
     5521 tgttctccag gctctcgatc acatcgacct cctggatcgg cgcctgatcc agatacgaat
     5581 acgtcttgac ggccttgacg atctccgaca tggcctcagc gctatgcccc acctcatcga
     5641 gcaagccgta gaccgaagaa ttggctccca accaccgaac gacgaagggc aactgctttg
     5701 gagagaagct gtcggccaat cgttccaact caaccctgtc ccagccgaag gagacaagca
     5761 cgggcgcgag ctcccacgcc tgcccgacgc catggtcctc cagccactcc tgcagttcat
     5821 cctcttgctc gctgagggtc agcgggtcgt acagaggcgc gacagtcgtg tgtttcatgg
     5881 cttctgcctg cagagtaccg aacgtatcgt tctgaagtgg ctcaccagcg atagagctaa
     5941 gttgggccgc caggcgctct ctttccgcca gcgcgtcacg tagctgtgcc gcgctacgcc
     6001 gtatggcggc ggccgggttg ttcaactcgt gcgccagtcc cgcggcaagc gtgccgaggg
     6061 ctgccat
//
LOCUS       DATAIR010000784         6610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_270787, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000784 DATAIR010000000
VERSION     DATAIR010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270787"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1584)
                     /locus_tag="VHM69_18605"
     CDS             complement(79..1584)
                     /locus_tag="VHM69_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2742458.1"
                     /translation="MDTSRGGAPGGTTEEVQEERVWHAGREDPMPIRELPEPPASLHI
                     LGPGMLLAALGVGLGETYLWPRLVILFGPEIRWLFFVGVTLQGVVMLEMARYATATGE
                     SIFFGAARLWRPIMWLFFSIAVLVYIWPGHVALGAQAFESLTGISWYIFAIFGLLLIG
                     VIISFSTIVYNVVEAILSTLIGVLVIGSTIIAALVGNLGFVWATITGLFTFGYFPQEA
                     ISGPQAAALFPLIVGAIAFAGPSGMQQMWYTLWLRDKGAGMGSYIPRIKGLTHMDEEE
                     TMPSRGFMFDTEDPENMRRWKGWRRWVHFDAWVLFWGITMVTTIIYTVLAQSALQIDP
                     GVREAILADDQGATLNGMAGAFAQAGGAVIGGLFFIFISVIGWKMTFGIFDAFSRGQA
                     DMTFFFIGPLRRLGMNRLYFAFLWFLILFGIAMLFVTGEEDGPAAILDILSVLSTFAM
                     GAYCLLLLGTNNLLLPKKIRPNIVINILLAFAALFYLGMLAYSYFRFGVIL"
     gene            2093..2221
                     /locus_tag="VHM69_18610"
     CDS             2093..2221
                     /locus_tag="VHM69_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742459.1"
                     /translation="MIHRIKKLISALAPSAQRFRASGKGMPGRGLGPRLTDRKHYK"
     gene            2354..3031
                     /locus_tag="VHM69_18615"
     CDS             2354..3031
                     /locus_tag="VHM69_18615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX2742460.1"
                     /translation="MIEPIRRVKLYEEVASRTRDLIVSGELQPGQALPSERKLAEQFK
                     VGRAVIREAIRRLEVSGLVESRHGGGNYVREVTVEHLVAPIATVLSDMGHLREELMEA
                     RLFFEPQIAREVAKSATPEDLRQLEDVIRRQEDRAKDGEATAEEDAEFHRLLASATHN
                     TVVVRVMEVIDNLLKDEHARWFQSAERSRNSLEGNRRILKAVQRRDQDAAQKAMEQHI
                     QEIASGF"
     gene            3195..3680
                     /locus_tag="VHM69_18620"
     CDS             3195..3680
                     /locus_tag="VHM69_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2742461.1"
                     /translation="MYRRILAAIDGSPACADTLEQAVRIARELDADLYTISVQEWPTP
                     LEVIGMEVEIPQTVHDEFFDECKYRIRSAAEEAGVTLKSTKVTKGNPAAAILRHIEET
                     GFDFVVLGRRGRGFIHRLRWTSTAHKVIACANCPVIVCPVCTDETMRIRRDVPQSSDV
                     V"
     gene            complement(3683..4180)
                     /locus_tag="VHM69_18625"
     CDS             complement(3683..4180)
                     /locus_tag="VHM69_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742462.1"
                     /translation="MNYSDLSAQNLEELREQITEYYETYYAELRSRLTSHDAATFQSV
                     PEVFKGYRRIVTELGQDGVVVTHWPSGQDSFDFHISPDRVAKDLAAEHCGGEQIMDYP
                     PGSDFGTYEITEPLQLVVDDRVVWVARWTRLEISSRLDAWLSTDQARTAARNDLLPFA
                     EARHS"
     gene            4477..5151
                     /locus_tag="VHM69_18630"
     CDS             4477..5151
                     /locus_tag="VHM69_18630"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEX2742463.1"
                     /translation="MLTRVRTVVLAAALVMVLACSCEVPVNSEKAGASSNTPVSEKLA
                     GLKVRPAGSMDGYSRELFSHWSDAQEYGWALPAGTPDPGSCDVRDAALIRDGRDEVVQ
                     QYCDVVSGTWFDPYGGATYTDPSDLDGDHFVPLANAYRSGASSWDAARRESFANIPRD
                     VLMVDDGLNQSKGDKGPEAWKPPRRAYWCTYAKKWVGIKYYWKLSVTSAEKSALKQML
                     ATCPSR"
     gene            5161..5484
                     /locus_tag="VHM69_18635"
     CDS             5161..5484
                     /locus_tag="VHM69_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742464.1"
                     /translation="MEPGSGVPHSGRRSVFASTTGGVMTREAGAVTGDLELATTVEGD
                     RLLAKVRYAGANEWYEIEGSPARVGAAGNPDEAHRLIVEHLGRPGPVTQGNEEPVSLE
                     GFTQA"
     gene            5586..6179
                     /locus_tag="VHM69_18640"
     CDS             5586..6179
                     /locus_tag="VHM69_18640"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2742465.1"
                     /translation="MDDMDTGSGQDPFAGLKESSDEYSVYDAHYERIGRVDDVLLDER
                     GAVSYIGVKMGFFGTNSTIVPVEIVRVNDRRHLIEIAEEAETIKHAPHFGHDETVSPE
                     LEDRVRTYFGLEPIQPSQEPEETHPTDDTSRFGPDDRVDTEHGERMEAQDRLAGESAS
                     EEEPAEAPPQESPGDSPEERWERETREGGMTVHRRKA"
     gene            6378..>6610
                     /locus_tag="VHM69_18645"
     CDS             6378..>6610
                     /locus_tag="VHM69_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742466.1"
                     /translation="MRTAVRLKFAIAPLCTNMKGPEEKGGTPLSSTGSPAVALLTAAK
                     MVRALIFGAIPAKLRSVHSDAFERYTAGSSSSGT"
BASE COUNT         1429 a         1995 c         2048 g         1138 t
ORIGIN      
        1 ggagtccggt gacgaaaccc gtcaccatcc ccggtatctt cagccgtcgc aagatgcgca
       61 ccgtctctgc ccccagccct acaggatgac cccgaagcgg aagtaactgt aggccagcat
      121 cccgagatag aagagcgccg cgaacgccag caagatgttg atgactatgt taggccggat
      181 cttcttcggc agaagcaggt tgttggtccc aagcagcagc aggcagtacg cgcccatcgc
      241 gaaggtcgag agtaccgaga ggatgtcgag gatggcggcg gggccgtcct cctcacccgt
      301 cacgaacagc atggcgatcc cgaacaggat caggaaccaa aggaacgcga agtagagcct
      361 gttcatccca aggcgacgca aaggcccgat gaagaagaac gtcatgtccg cctgaccgcg
      421 cgagaaagcg tcgaagatgc cgaaggtcat cttccagccg atcaccgata tgaagatgaa
      481 gaacaggccg cctatgaccg caccgcccgc ctgggcgaag gcgccagcca tgccgttgag
      541 agtcgccccc tggtcatcgg caaggatggc ctccctcacg ccggggtcga tctgcagcgc
      601 actctgcgcg agcacggtgt agatgatggt ggtaaccatc gttatccccc agaacaaaac
      661 ccaggcgtcg aagtgaaccc agcgacgcca gcctttccag cgccgcatgt tctccggatc
      721 ctccgtgtcg aacatgaagc cccgggaggg catggtctcc tcttcatcca tgtgggtcag
      781 gcccttgatc ctggggatgt acgaccccat gccagcaccc ttgtcccgca gccacagcgt
      841 gtaccacatc tgctgcatcc ccgaaggacc ggcgaaggcg atggccccca cgatcagcgg
      901 gaacagcgct gcggcctggg gaccactgat cgcttcctgc ggaaagtatc cgaaggtgaa
      961 tagacccgtg atggtggccc atacgaaccc caggtttccg acgagagccg cgataatagt
     1021 ggagccgata acgagtactc cgatgagggt cgacagaatg gcttcgacga cgttgtagac
     1081 gatcgtcgag aagctgatta tgacaccgat gaggagcagc ccgaatatgg caaatatgta
     1141 ccaactgatc cccgtcaggc tctcgaacgc ctgggccccg agcgccacgt gtccaggcca
     1201 gatgtagacc aacaccgcta tggagaaaaa tagccacatt atcggacgcc agaggcgtgc
     1261 cgccccgaag aagatgctct cgccggtggc cgtcgcatag cgcgccatct ccagcatgac
     1321 gaccccctgg agcgtgacgc cgacgaagaa gagccagcgg atctccggtc cgaagagtat
     1381 gacgagccgc ggccatagat acgtctcgcc tagacccacc cccagagccg ccaggagcat
     1441 gccaggcccg aggatgtgca acgaggcagg gggctcaggc aactccctga tcggcatcgg
     1501 gtcttccctg cccgcgtgcc acaccctctc ttcctgtact tcctcggtag ttcccccggg
     1561 cgcaccaccc ctagacgtgt ccaccacaat ctcctttcat acccggatcg gttagtgccc
     1621 gacgtgcctg cgatcgaacg tcacagacat cgaccgaatg gtcagatgca ctccacgccg
     1681 cgtgtgaccc cgaacagcga acttccacga ccgtccaatg cctttcccag agccgtgaaa
     1741 acgagcgcca atgcagagca tccttacccg attttgctaa ccgacctatc tgcctttccc
     1801 tcttgcgaca ggtcatacca gcatacacac gggtctgatt tgtgtcaaga agaacattct
     1861 ccagcccgaa gccccccttt gcccccgcaa aactcgcaac cgagacctgt tgtaccctcg
     1921 ccagcagtgc cacgtagggt cccgaacctc actttgtgat atctttctca ccccttgaca
     1981 ctatttgctt gactgttgat acaagctcgc tacaatcaag atcaagaact ggacatacca
     2041 gtttggaaga ccgaaatgac ggaagattca gagaagaaga gaggagtgga ccttgataca
     2101 taggatcaag aagttgataa gtgctttggc acccagcgcg cagcgctttc gtgcgtcggg
     2161 caagggtatg cctggcaggg gccttggacc gaggctcacc gaccgcaagc actacaagta
     2221 ggctggcgca acgctggctt cctcggcccc cacccgtcga tgcgggtggg ggccgaatgc
     2281 tatgggctgg ctcgggtatg aaggtgctaa actgaactaa atggaaccga cgggaactga
     2341 cacgaacgag ccagtgatcg agccgatccg ccgcgtcaag ctctacgaag aagtggcgtc
     2401 ccggacccgc gacctcatag tcagtggcga gctccagcct gggcaggcgt tgccttcgga
     2461 gaggaagctt gcagagcaat tcaaggtagg ccgcgcggtg atacgggagg cgatccgccg
     2521 tcttgaggtg tccggcctcg tggagagccg ccacggtggt ggcaactacg tacgggaggt
     2581 caccgtcgag catctggtcg ctcccatcgc caccgtgttg agcgacatgg ggcaccttcg
     2641 cgaggagctc atggaggcga ggttgttctt cgagccgcag atagcgcggg aagtcgccaa
     2701 gagcgctacg ccggaggatc tgcggcagtt ggaggacgtc atacgccgtc aggaagaccg
     2761 cgcaaaggat ggcgaagcga ccgcggagga ggacgcggag tttcatagac ttctggcgag
     2821 cgcgacccac aacacggtgg tcgtacgggt gatggaggtg atcgacaacc ttctcaagga
     2881 cgagcacgcg aggtggttcc agtccgccga gcggtcgagg aattcgctcg agggcaaccg
     2941 ccgtatcctg aaggccgtcc agcgtcgcga tcaagatgcc gcacagaagg ccatggagca
     3001 gcatatccag gagatagcta gcggcttcta gtcgattgtc gtagcagccg ctcggacatc
     3061 cctactcaac cgcacaaacc ttcgatcaaa tcgtggtcat accaatagag cgatgcattg
     3121 ctacgttttg ctctaatgct ataatgccta catctcgagg taacatgggg tgcgagtatc
     3181 gaagggtgat aggcatgtac agaagaatac tggcggccat tgacggatcg ccggcatgcg
     3241 ccgacaccct ggagcaagcg gtacggattg ctcgggagtt ggacgccgat ctctatacaa
     3301 tctcagtaca ggagtggccc acaccgctcg aggtcatagg gatggaggta gagataccgc
     3361 aaacggtgca cgacgaattc ttcgacgagt gcaagtatcg aatccggtcc gcggcggagg
     3421 aggccggggt cacgttgaag tcgaccaagg tgaccaaagg aaacccggcg gcggcgattt
     3481 tgcgtcacat cgaggagacg ggttttgact ttgtcgtgct tggccggcga ggccggggat
     3541 tcattcaccg tttgcgctgg acatccacgg cccacaaagt catagcgtgc gcgaactgtc
     3601 cggtgatagt ctgcccggtc tgtaccgacg agactatgag gataaggcgg gacgtgccgc
     3661 aatcctcaga cgttgtatga cgctagctgt gtcgagcttc cgcgaagggg agaaggtcat
     3721 ttctggcggc tgtccgcgct tgatccgtgc tcagccaggc gtcgagcctg ctcgagatct
     3781 ctagacgcgt ccacctggct acccagacga cccgatcgtc gaccacgagc tggaggggtt
     3841 ccgtgatctc gtaggtcccg aaatcgctcc ccggcggata atccatgatc tgttcaccgc
     3901 cgcagtgttc ggctgccagg tccttcgcca cccggtcggg agagatgtgg aaatcgaacg
     3961 aatcctggcc gctgggccag tgggtcacga ccaccccgtc ctggccgagc tcggtgacga
     4021 tccttctgta ccccttgaaa acctcgggaa cggactggaa ggttgccgca tcatggctcg
     4081 ttaaccggct ccgcagttca gcgtagtagg tctcgtagta ctcggtgatc tgttccctca
     4141 gctcttcgag gttctgggcg ctcaggtcac tgtagttcat cgttacctcc tatggatcag
     4201 gcggtctctc ctcgagatcg tccgaccaga cccctccgta accgggtacg ctggagtcga
     4261 actcccgcct ggttacgcta gtccattgaa acatagccac gaggatggga cacgtctccg
     4321 ggccatcaga ccggcgccat ggtatcgtcc acggcgccgg tcctctgatt acgccggcga
     4381 gccggtactg tagaatcctc gccgggttgc gacagcggta cgacgagcag gaaggaagcg
     4441 ctgctggtgg agttgagaga gcggcgagag gatcgcgtgt taaccagggt caggacggtg
     4501 gtactggcag cggcgctcgt gatggtcctg gcgtgctcct gcgaagtacc tgtcaactcg
     4561 gagaaggcag gggcgtcttc gaataccccc gtctccgaga agctcgctgg cctgaaggtg
     4621 aggcccgccg gctctatgga tggctactcc agggagctct tctcccactg gtcggacgcc
     4681 caggagtacg ggtgggccct gcccgcgggg acaccagacc ccggctcctg cgacgtgagg
     4741 gacgcggccc tcatccgcga cggccgggac gaggtggtcc agcagtactg cgacgtcgtg
     4801 agcgggacgt ggttcgaccc ttacggcggg gcgacctaca ccgacccttc cgacctggac
     4861 ggcgaccact tcgtccccct ggccaacgcc tacaggagcg gagcctcctc gtgggatgct
     4921 gcgaggcgcg agagtttcgc caatatccca agagacgtgc tcatggtcga cgacggactc
     4981 aaccagtcca agggagacaa gggacccgag gcctggaaac ccccgagaag ggcctactgg
     5041 tgtacctacg ccaaaaagtg ggtggggatc aaatactact ggaagctatc cgtgaccagc
     5101 gcggagaaga gcgccctgaa gcagatgctc gcaacctgcc cctcgcgatg agggtctgag
     5161 atggagcctg gatcaggcgt tccgcactcc gggcggcgct cggtcttcgc ctcgacgacc
     5221 gggggtgtca tgaccagaga ggccggcgcg gtcacgggcg acctcgaact tgcaaccacg
     5281 gtggagggag atcgtctctt agcgaaggtg cgttacgccg gcgcgaacga atggtacgag
     5341 atagagggct ctcccgcccg ggtcggggct gccggcaatc ccgatgaagc gcatcgcctc
     5401 atcgtcgagc acctcgggcg tccaggcccc gtcacgcaag gaaacgaaga acccgtatcg
     5461 ctcgaaggct tcactcaggc gtaacggaag cgcgttccgc ccggtcaata ttagtagaat
     5521 gacaaagtcg ggaacggtgg tggctctctc gacgtgcaga ccaaggtttc ttgagaggat
     5581 gagacatgga cgacatggac acaggaagcg gccaggaccc cttcgcgggg ctcaaggaaa
     5641 gcagtgacga gtacagcgtc tacgacgcgc actacgagag gatcggcagg gtagacgacg
     5701 tcctcctcga cgagcggggc gcggtgtctt acatcggcgt gaagatgggc ttcttcggaa
     5761 ccaactccac catcgtaccg gtcgagatcg tcagggtaaa cgacagacgg cacctgatcg
     5821 agatcgccga ggaggccgag acgatcaaac acgctcccca cttcggtcac gacgagaccg
     5881 tgtcaccaga actggaagac agggtgcgga cctacttcgg cctcgaaccc attcagccct
     5941 cccaggaacc ggaagagacg caccctaccg acgacaccag ccgcttcggt cccgacgacc
     6001 gggtggacac cgaacatggc gagaggatgg aggcccagga tcgtctcgcc ggggagtccg
     6061 ccagcgaaga ggaaccagca gaagcacctc cccaggaaag tcctggcgac agcccggagg
     6121 aacgctggga gcgggagacg agggagggtg gcatgacggt ccaccgccgc aaagcgtagg
     6181 taggagatgg actgagggtc gtgcctgatt cgcggcgcac ggacggcatg tgagggtctg
     6241 ggacctcgcg ccagcctgag tccgtatcca gccgctcgtc ttctgcaagg agatccagga
     6301 gccctgcgaa gatcgtttcc gagtcagtca ccccgagcaa accggagtac gcctcgtcgc
     6361 tcaaggaatc gcgaagggtg cgcactgccg tccgtctgaa gttcgctatc gcgccgttgt
     6421 gcacgaacat gaaaggtcct gaagagaagg gcgggacccc gctttcctcg acgggcagtc
     6481 ccgccgtcgc gctcctgacg gcggcgaaga tggtgcgggc cctgatcttc ggggcgatcc
     6541 cggcgaagct ccgatcagtc catagcgacg cgttcgagcg atacacggcc ggctcgtcgt
     6601 cctcaggcac
//
LOCUS       DATAIR010000785         5528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_76908, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000785 DATAIR010000000
VERSION     DATAIR010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76908"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VHM69_18650"
     CDS             <1..831
                     /locus_tag="VHM69_18650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742467.1"
                     /translation="LGALFGFFTAWGLTRRIGRLARAARSWSRGDFSVTTKDRSKDEL
                     GQLSRELNSMAAELENLLQARGELATLEARNRFARDLHDSVKQQVFATSLQIAAARAL
                     IEKDKNSAESHLSQADELVRQAQKELNVLIGEMRPAALEGKGLAAALREYVARWSEGA
                     EIPAEFHVQGEREVSLEVEQALFRVAQEALANVARHSGAGHAEVDLTYTAGTITLRVA
                     DDGRGFDTARGPGAGFGLQSMRERLVRLGGHVNVESAPGKGTRVECVCPDPSEKGKKV
                     "
     gene            828..1481
                     /locus_tag="VHM69_18655"
     CDS             828..1481
                     /locus_tag="VHM69_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742468.1"
                     /translation="MSEPITVLLVDDHALVRQGVRAFLETQDDISVVAEASGGEEAVK
                     LAAEHAPDVALMDLIMPGMDGVEATRRLVAISPRTNVVMLTSYHDDEHVFPAIRAGAL
                     SYVLKEIGPDELADAVRKAAAGEAVLHPRVAARVVRELHGARRTEPNVFHDLSDREME
                     VLKLIAEGLHNSEIAARLYVSEKTVKSHVSNILSKLHLADRTQAAVYAWRQGVVRRE"
     gene            complement(1533..2123)
                     /locus_tag="VHM69_18660"
     CDS             complement(1533..2123)
                     /locus_tag="VHM69_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2742469.1"
                     /translation="MASKSGRQSRGSGGSRRTYIIVGAIAVLFVGGFIALAVVDARQQ
                     SASGAPDDVQTYDVGPAGQHTGGDVDYEQSPPTGGAHNPVWQNCGYYDKPVRDENAVH
                     SLEHGAVWITYSPDLPKGQVDELRNLAQSQSYILVSPYSDLPDNAPVVASAWGKQVLL
                     DGADDPDLESFISAYRQGPQTPEPAAVCTGGTSATQ"
     gene            complement(2187..2567)
                     /locus_tag="VHM69_18665"
     CDS             complement(2187..2567)
                     /locus_tag="VHM69_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX2742470.1"
                     /translation="MSFVERLKRWARRLKVEVHALYLAYKDPRVPWYARVFAAVVVGY
                     AFSPIDLIPDPIPVLGYLDDLVLIPLGVALAIRMIPPHVLAECREEARQAKDRPVSRV
                     AAVVVVAVWVALAALAVWFLARAF"
     gene            complement(2564..2770)
                     /locus_tag="VHM69_18670"
     CDS             complement(2564..2770)
                     /locus_tag="VHM69_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742471.1"
                     /translation="MGELENGYEFAFPGDAEWAMKLTEFIVAERSCCPFFTFELAFET
                     DGILLRVRGPQGTRQFIREHASLR"
     gene            complement(2893..3582)
                     /locus_tag="VHM69_18675"
     CDS             complement(2893..3582)
                     /locus_tag="VHM69_18675"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2742472.1"
                     /translation="MSIQPLQLSRKLPGTVERERLVRRAKFLAWLGVGWHGVEAAIAV
                     GAGLVAGSIALVGFGADSLIESFAGIILLWRFAASRATSESAERRAQKLIGLSFYVIA
                     GYVGFEAVRSLLSGERPEASWIGIGLAAITLVTMPPLAIAKARVGEKLGSSATRSEGQ
                     QNMLCAYLSAALLVGLGANALFGLWWADPVTALLIAGVAIKEGRESWRGESCCTAPVL
                     PGEQCEDDCCG"
     gene            complement(3579..3938)
                     /locus_tag="VHM69_18680"
     CDS             complement(3579..3938)
                     /locus_tag="VHM69_18680"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2742473.1"
                     /translation="MNEERCDLLCLDAPRAEAIREGLLDVEAALEAAERARMLSDPTR
                     LVLAAALGEGGELCVCDLAWISSRAQNLVSHHLRLLRSHRLVASRRSGKLVMYSLTDE
                     GRSLLRAVLGEGATVAS"
     gene            complement(3987..4904)
                     /locus_tag="VHM69_18685"
     CDS             complement(3987..4904)
                     /locus_tag="VHM69_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2742474.1"
                     /translation="MGGHEHGTHQRGSSLRALALVLALTASFTVVEIIGGLLTGSLAL
                     LADAGHMLSDNLSLGLALFAAWLATRPATPEKSFGYRRAEILAALANGITLVAISIWV
                     FVEAFSRLREPTEVLGGPMLAVAALGLLVNAVGAIVLYRSGGESLNVDGAMRHVLADA
                     LGSVGAMAAAAVIILTGWRYADPLISIAIGLLILGSSWTLLRDSTNILLEATPRNLKA
                     EEVGRKMAAADGVVEVHDLHIWTITSGFPALAAHVLVGTDEDCHARRRELEGLLSHDF
                     GISHTTLQVDHVGDHHASLQTLRFPSHND"
     gene            complement(4928..5155)
                     /locus_tag="VHM69_18690"
     CDS             complement(4928..5155)
                     /locus_tag="VHM69_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742475.1"
                     /translation="MWARRIKETFAILTIGDGMIELISPREHSLLWEFGPDSIRNVAR
                     FFADNPNLMRLLGILQVAFGLWLALRQYRER"
     gene            complement(5168..>5528)
                     /locus_tag="VHM69_18695"
     CDS             complement(5168..>5528)
                     /locus_tag="VHM69_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX2742476.1"
                     /translation="AIPSDTAVSVADQLIETGEVTTPYLGILTTDLSPEDAGRFDLPV
                     DSGALVEQVVPGSAADEAGVRRGDIITALGDAKVESYGDLLGALRDYEPGDRVSLTVF
                     RNGDEKYLEVTLGENPQ"
BASE COUNT          969 a         1788 c         1824 g          947 t
ORIGIN      
        1 ctgggtgccc tcttcggctt ctttacggcc tggggcctga cgcgtcgcat agggcgtctc
       61 gccagggcgg cccggtcgtg gagccggggc gacttctcgg tgacgacgaa agaccgctcg
      121 aaggacgagc tcggccagct cagtcgggag ctcaacagca tggcggcgga gctcgaaaac
      181 cttctccagg cgcggggcga gctcgcgacg ctcgaagccc gcaaccgctt cgcccgcgac
      241 ctgcacgact ccgtcaagca gcaggtcttc gcgacctcgc tccagatcgc cgccgcccga
      301 gccttgatcg aaaaggacaa gaactcggca gagtcccacc tctcccaggc cgacgagctc
      361 gtgcgccagg cacagaagga gctgaacgtc ctgatagggg agatgcgtcc ggccgccctc
      421 gaagggaagg gtctcgccgc ggcgctacga gagtacgtcg cccgctggtc ggaaggagcc
      481 gagatacccg ccgaattcca cgtccagggt gagcgagagg tatctctgga agtcgaacag
      541 gcactcttcc gcgtcgccca ggaggcgctc gcgaacgtcg ccaggcacag cggagccggg
      601 cacgctgagg tggacctgac ctatacggcg ggcaccatca ccctgcgcgt cgccgacgac
      661 ggcaggggct tcgacaccgc tcgtggtccg ggcgcgggct tcggcctcca gagcatgcgc
      721 gagcggctgg tcaggctcgg cggacacgta aacgtcgaga gcgcgcccgg caaggggacg
      781 agggtggaat gcgtctgccc cgatccttca gagaagggaa agaaagtatg agcgagccga
      841 tcaccgtgtt gctggtagac gatcatgcgc tcgtgcggca gggcgtacgc gccttcctcg
      901 agacgcaaga cgacatcagc gtggtcgcag aggcctcagg cggagaggag gccgtgaagc
      961 tcgccgccga gcacgccccc gacgtcgccc tgatggacct gatcatgcct ggtatggacg
     1021 gcgtggaggc gacgaggcgc ctggtagcga tctccccgcg tacgaacgtc gtcatgctca
     1081 cctcctatca cgacgacgag cacgtcttcc cagcgatccg ggccggcgcc ctctcctacg
     1141 tcctcaagga gatcggcccc gacgagctcg cggacgccgt ccgcaaggcc gcggcgggcg
     1201 aagcggtgct gcaccctcgc gtagcggccc gcgtggtcag ggagcttcac ggcgccaggc
     1261 gcaccgagcc aaacgtcttc cacgaccttt cagacaggga aatggaggtc ctcaagttga
     1321 tcgccgaagg actgcacaac tccgaaattg cggcccgtct ctacgtgagc gagaagacgg
     1381 tcaagagcca cgtctcgaat atcctttcga agttgcacct cgccgaccgt actcaggccg
     1441 ccgtctacgc ctggcgccag ggcgtcgtcc gccgcgagtg atcagggctt gttcgaggat
     1501 ttgcctccga acgagcctgg cttcaggtgt tgctattgcg tggcgctcgt gccgccggtg
     1561 cagactgcgg ctggttcggg ggtctgtggt ccctggcggt aagcgctgat gaagctctcc
     1621 aggtcggggt cgtcagcgcc gtccagtagg acctgtttgc cccaggccga ggcgacgact
     1681 ggggcgttgt caggcaggtc ggaatagggg ctgaccagga tgtatgactg gctctgtgcg
     1741 aggttgcgca gctcgtctac ctgacctttc ggaaggtcgg gggagtaggt gatccagacc
     1801 gcaccgtgtt ccagagagtg cacggcgttc tcgtcgcgga caggcttgtc gtagtagccg
     1861 cagttctgcc agacggggtt gtgcgctcct ccggtgggag ggctctgctc gtagtccacg
     1921 tctccccctg tgtgctgccc ggccgggccg acgtcgtagg tctgtacgtc gtcaggggcg
     1981 ccgctggccg actgctgcct ggcgtccacc acagcgagcg cgatgaaccc gccaacgaag
     2041 agtacagcga tggccccgac tattatgtag gtccttctgg aacccccgga acctctgctc
     2101 tgtcgaccgc tcttgctcgc caagtaatat ccttcttcta gcctgaggga gatcgtagca
     2161 cacgccgccc gtctctccga ggggcctcag aacgcacgcg ccagaaacca cacggccagc
     2221 gccgcgaggg cgacccagac ggccacaact accacggcgg ctaccctgct gaccggcctg
     2281 tccttcgcct gacgagcctc ttcgcggcac tcggcgagga cgtggggtgg gatcattcgt
     2341 atcgcgagcg ccaccccgag cgggattagc accagatcgt cgaggtaccc gaggacgggg
     2401 atcgggtccg gtatgaggtc tatggggctg aaggcgtacc cgacgacgac ggcggcgaag
     2461 acccgcgcgt accacggcac cctcgggtcc ttgtaggcga gatacaacgc atggacctcg
     2521 accttgagcc tgcgcgccca ccgcttgagc cgctcgacga agctcaccgc agggacgcgt
     2581 gttctctgat gaactgcctg gtgccctgcg gtcctctgac gcgcagcagg atcccatcgg
     2641 tctcgaaagc gagctcgaag gtgaaaaacg gacagcagga acgctcggct actatgaact
     2701 cggtgagctt cattgcccac tccgcatccc ccggaaaagc gaactcgtag ccattttcga
     2761 gctcgcccac gagacggctc ctgttcagaa gatctctgac cgcttcatgt cgccggactt
     2821 gctcgtctcc ggagaggttg caggcgatcg gcggttctcc tctggaaagt tgggtgctca
     2881 cttttctcgc acttatccgc agcagtcgtc ttcgcattgt tcaccgggga ggacaggcgc
     2941 ggtgcagcag ctctcgccgc gccacgactc gcggccctcc ttgatggcga cgccggcgat
     3001 gagcagcgcc gtgacagggt cggcccacca caggccgaag agcgcgttcg ccccgagtcc
     3061 cacgagcagt gccgcggaca aataggcgca gagcatgttc tgctgcccct cgctcctggt
     3121 cgccgaggaa ccgagcttct cgccgacgcg ggccttcgcg atagcgagcg gaggcatggt
     3181 caccagcgtg atagcagcga gaccaatgcc gatccaactc gcctcgggcc gctcgccact
     3241 taacaaactc ctcaccgcct cgaagcctac gtatccggca atgacgtaga agctgaggcc
     3301 gatgagcttc tgggcgcgac gttccgcgct ctccgaggtg gcccgcgagg ctgcgaatcg
     3361 ccagagcaag ataatccccg cgaacgactc gatgagcgag tcggccccga aaccgacgag
     3421 cgcgatagac cccgcgacga gcccggcgcc gaccgcgatg gccgcctcta cgccgtgcca
     3481 gcccaccccg agccacgcca ggaatttcgc ccgcctgacc agcctttccc tctcgaccgt
     3541 ccctggcagc ttgcgtgaca actggagcgg ctggatactc acgaggccac cgttgcccct
     3601 tcgcccaaaa cggcccgcag gagcgagcgt ccctcatccg tgagtgaata catgaccagc
     3661 tttccgcttc tccgggaggc cacgagccgg tgcgagcgga gcaacctcag atggtgtgag
     3721 acgaggttct gggcgcgaga agagatccag gccaggtcgc atacgcatag ctccccaccc
     3781 tccccgagcg cagccgcaag caccagccgc gtcgggtcgg aaagcatcct cgcacgctcg
     3841 gccgcctcaa gggcagcctc cacgtcgagc aacccctcgc gtatcgcctc cgcgcgcggg
     3901 gcgtcgagac atagtagatc gcaacgctcc tcattcatat cgtcattcta acgcgtgttg
     3961 atacgttggt gcaagcggga tgaacgtcag tcgttgtggg aggggaagcg gagggtctgg
     4021 agggatgcgt gatggtcgcc cacgtggtcc acctggaggg ttgtatggga gatgccgaag
     4081 tcgtgggaga ggagaccttc tagttcgcgg cggcgggcgt ggcagtcttc gtcggtgccg
     4141 acgaggacgt gggctgcgag ggctggaaag cccgatgtga tggtccagat gtggaggtcg
     4201 tggacctcga ccacgccgtc tgcggcggcc atcttccggc ccacttcctc ggctttcagg
     4261 ttacggggtg tcgcttccag caggatgttc gtggagtctc tcaggagcgt ccaggagcta
     4321 cccaggatca ggagcccgat ggcgatgctg atcagggggt ccgcgtagcg ccagccggtg
     4381 aggatgatga cggctgcggc ggccatcgcc ccgaccgagc ctagcgcgtc ggcgagtaca
     4441 tggcgcatgg cgccatccac gttcaaactc tcgcccccgg atcggtagag gacgatagcg
     4501 cccaccgcgt tgacgaggag cccgagcgcg gcgacggcca gcatcggccc gccgaggacc
     4561 tcggttggtt ccctgaggcg cgagaaggct tccacgaaga cccagatcga tatcgccacg
     4621 agtgttatgc cgttcgcgag ggcggccagt atttcggccc gcctgtagcc gaagctcttc
     4681 tccggggtcg ctggcctcgt tgccagccag gcggcgaaga gtgcgaggcc gagcgagagg
     4741 ttgtcggaga gcatgtggcc tgcgtccgcg agcagggcga ggcttcctgt gaggaggccg
     4801 ccgatgatct cgactacagt gaagctcgcg gtcagggcga gcacgagggc gagcgcgcga
     4861 agggaactcc cgcgctggtg cgttccgtgt tcgtggccgc ccatgacgtt attctatcgc
     4921 tgcgtggcta tctttcgcgg tactggcgca gcgccagcca taggccgaag gccacctgga
     4981 gtatgccgag gaggcgcatc aggttcgggt tatccgcgaa gaagcgggcg acgtttcgga
     5041 tactatcggg gccgaactcc caaaggagcg agtgttctct gggggagatc agctcgatca
     5101 tcccgtcacc tatggtgagg atagcgaagg tctccttgat tctgcgggcc cacatgtggc
     5161 tcctccttta ctgcggattc tctccgagag tcacttcgag gtacttctcg tccccgttgc
     5221 ggaagaccgt caggctgacc ctgtcgcccg gctcatagtc gcgcagggcg cccaggaggt
     5281 ctccgtagct ctctactttg gcgtcgccaa gggccgtgat tatgtccccc cggcgcaccc
     5341 cggcctcgtc ggccgcactg cctggcacga cctgctccac cagcgcgccg gagtctacag
     5401 gtaggtcgaa tcgtcccgcg tcctcggggc tcaggtccgt cgtcaggatt ccaaggtagg
     5461 gggtggtgac ctcgcctgtc tcgatcagct ggtctgccac agagaccgcc gtgtcggacg
     5521 gtatggcg
//
LOCUS       DATAIR010000786         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_168923, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000786 DATAIR010000000
VERSION     DATAIR010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168923"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHM69_18700"
     CDS             complement(<1..220)
                     /locus_tag="VHM69_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HEX2742477.1"
                     /translation="METEMVMEAPGRALKLDPIKSIKKAPAEEFYQSGHRTCQGCLSA
                     LPMRLMAKAAGPRTIVLGTTGCMYVANTT"
     gene            complement(273..1493)
                     /locus_tag="VHM69_18705"
     CDS             complement(273..1493)
                     /locus_tag="VHM69_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX2742478.1"
                     /translation="MTITTPSGGVKQTEKLMTGTEAVAQAVRLADVDVMAAYPIRPYD
                     GVMQAVAKLIANGELDAEYIVAEGEHSQFEICKHASAVGSRVFVGSSGVGWMYAMESL
                     AVTPTLRLPVVAMIGNRALDDPGAFGVEHNDAMCVRDLGWQLFWVEDAQECFDATLMA
                     YRVGEDKRVSFPVGLGVDGAFITHSQALVKIPDQESVNAFLPPYDLGDRLLHPDNPIS
                     IAPQANEDWVMEIRKQTDEAAWRVKDVIREVHADFRELFGRGGDNPFFEEYRTEDADY
                     VLLGMGSLGLPAKVMVRRLRDRGEKVGFIRLKWFRPFPDVELAEVLGRFDAVGVVDRD
                     YSYGSPSQGGVLYTDLRAALYNAEQRPRMVNFIGGLGGRELTPEMRDEMALITRKAAD
                     GEEVPDVSWIGVRE"
     gene            complement(1505..2680)
                     /locus_tag="VHM69_18710"
     CDS             complement(1505..2680)
                     /locus_tag="VHM69_18710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEX2742479.1"
                     /translation="MPTGREVGDSIPGDQDTIEGRTSTEVSEEAKSVEFTYRGIFQKT
                     LAANLCKHVVMAARKRDWLGSTIQRYSDSPERNGIPAKQFAVIAPDRETLEGHMAKYE
                     PNELDVITVLDDTMCDGIESWAWYGRQPINVRLRRGGTLVVISNRSPEELLKVIPQKP
                     WDWTLAIVPGTPSMGGLWVYRDDGTDMRVLGAIASARPDIFGLDAVSAVVRDDESLDD
                     RYLDALRSGHDEVSVYEVSAGEGKVDDYEPVKLPGWKDMREAIIVEAVKPGERNPHYR
                     SSSDRTMRPVVNFDTCIKCRQCWIDCPDECFDISDEELHPINYSYCTGCGICANVCPV
                     EDCIVMVNDLEFIEEGDKDVFPMWKEDKDSYHEWREEKIASGRIDHQAHFNTDTWAK"
     gene            complement(2878..3297)
                     /locus_tag="VHM69_18715"
     CDS             complement(2878..3297)
                     /locus_tag="VHM69_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742480.1"
                     /translation="MNSSSSQVPRFVFVAFYPEGPHAGPISIGLFGVQDGVLLPVFSS
                     REKAINFAVRVLGAPPQKNENWDGVELPAEDLIQLLRADASISYVAADPAPSGEVELI
                     PTPEFIERLEQEAGDPDNPSDDFRRSLVRPFVYGDVS"
     gene            3581..>3905
                     /locus_tag="VHM69_18720"
     CDS             3581..>3905
                     /locus_tag="VHM69_18720"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX2742481.1"
                     /translation="MKINKWTRRHANSEEDNAPPEAVPDSGEEVHQPPDGLSEAGIRE
                     ALRDVRDPEIGRDLISLNMIRSIHIDASHVTVGVALTTAGCPMKHRIITDITDRLTII
                     DGVDEV"
BASE COUNT          714 a         1275 c         1144 g          772 t
ORIGIN      
        1 aggtcgtgtt cgccacgtac atgcaccccg tggtaccgag aacgatcgtg cgcgggcccg
       61 ccgccttcgc catcagcctc atgggcaggg cggacagaca accctggcat gtacgatggc
      121 cggactggta gaactcctcc gccggcgcct tctttatcga tttgatcgga tcgagcttga
      181 gcgcccgtcc tggagcctcc ataaccatct ctgtctccaa tctccttcct gcgctctact
      241 tctactcgct actttcttta ttttcctcag ccctactcgc gcaccccgat ccaactgacg
      301 tcgggcacct cttcgccatc ggctgccttc ctggtgatga gcgccatctc gtccctcatc
      361 tcgggggtaa gctcgcgacc gccaaggccc ccgatgaagt tgaccatacg gggccgctgc
      421 tcggcgttgt acagggcagc gcgcaggtcg gtgtagagaa caccgccctg agacggcgaa
      481 ccgtaggagt agtcgcggtc gaccacgcca acggcatcga agcggccaag cacctccgcc
      541 agttccacat cggggaacgg cctgaaccac ttgaggcgga tgaaacccac cttctcgccc
      601 ctgtcccgca ggcggcgaac catgactttg gccggcaggc cgagcgaacc catgccaagg
      661 agcacgtagt cggcgtcctc ggtcctgtac tcctcgaaga agggattgtc accgccgcgt
      721 ccgaagagct ccctaaagtc ggcgtggacc tcgcggatca catccttcac cctccacgcg
      781 gcctcgtcgg tctgcttgcg gatctccatt acccagtcct cgttcgcctg gggggcgatg
      841 gagatggggt tgtcgggatg gagcagccgg tctcccagat cgtacggcgg caagaacgcg
      901 ttgacgctct cctggtccgg gatcttgacg agcgcctggg aatgcgtgat gaacgccccg
      961 tccacgccca atccaaccgg gaaggagacc ctcttgtcct cgccgacgcg gtacgccata
     1021 agcgtcgcgt cgaagcactc ctgcgcgtcc tcgacccaga agagctgcca gcccaggtca
     1081 cgcacgcaca tcgcgtcgtt gtgctcgacg ccgaaagccc caggatcgtc aagtgcgcgg
     1141 ttcccgatca tggccaccac cgggagcctg agcgtcgggg tgacagccag agactccatg
     1201 gcgtacatcc agccaacacc cgaggaaccg acgaacaccc ggctgcctac ggccgaagca
     1261 tgcttgcaga tctcgaactg ggagtgctcg ccctcggcga cgatgtactc ggcgtcgagc
     1321 tcgccgttgg cgatcagctt cgccacggcc tgcatgaccc cgtcataggg gcgaattggg
     1381 taggccgcca tcacgtcgac gtccgcgaga cgaaccgcct gcgcgacggc ctccgtgccg
     1441 gtcatcagtt tttcggtctg cttgacccct ccagagggtg tcgtgatggt catcgtgatc
     1501 ctccctactt cgcccacgta tccgtgttga agtgcgcctg gtggtctatc ctgccactgg
     1561 cgatcttctc ctcacgccac tcgtggtagg agtccttgtc ctctttccac atcgggaaga
     1621 cgtccttgtc gccctcctcg atgaactcga ggtcgttgac catcacgatg caatcctcga
     1681 cagggcaaac gttggcgcag atgccgcacc ctgtacagta gctgtagttg atcggatgca
     1741 gctcttcatc ggagatgtcg aagcactcgt cggggcagtc gatccagcac tggcggcact
     1801 tgatgcacgt gtcgaagttg acgacgggcc gcatggtccg gtctgaagag gacctgtagt
     1861 gcgggttgcg ctcgccaggc ttgaccgcct cgacgatgat cgcctcgcgc atgtccttcc
     1921 acccaggcag cttgacaggc tcgtagtcgt ccactttgcc ctcgccggcg gagacctcgt
     1981 agacactcac ctcgtcgtgc ccgctcctga gcgcgtcgag gtacctgtcg tcgaggctct
     2041 cgtcgtccct gacgacggcc gataccgcat ccaacccgaa gatgtcgggc ctggcgctcg
     2101 ctatggcgcc gagcactcgc atgtccgtgc cgtcgtcgcg gtagacccag agcccaccca
     2161 tgctgggcgt ccctggtacg atcgccaggg tccagtccca cggcttctgc gggatgacct
     2221 tgagcaattc ctcgggactc ctgttcgaga tcacaaccag cgtcccaccg cgccgcaggc
     2281 gcacgttgat cggctgtcgc ccgtaccagg cccaggactc gatcccgtcg cacatcgtgt
     2341 cgtccaggac cgtgatgacg tccagctcgt tgggctcgta tttggccatg tggccttcca
     2401 acgtctccct atccggcgcg atgaccgcga actgcttggc cgggatgcca ttgcgctcag
     2461 gcgaatcact gtagcgctgg atggtcgatc ctaaccagtc ccgcttgcgg gccgccataa
     2521 ctacatgctt gcacaggttc gcagctaacg tcttctggaa gatcccccga tacgtgaact
     2581 cgacgctctt ggcctcctcg cttacctccg tggaggtgcg cccctcaatg gtgtcttgat
     2641 ccccgggaat cgagtcgcca acttctctgc cagtgggcat ccgttctcct tccctagtaa
     2701 tcggcccccc agagccaact cctacacccc tcagcttcgg tggtcgaagc tctggcctga
     2761 ccactttacc aatttgcaat gcatcctatc agcggcgggt aacagggtca atgccatgta
     2821 ccggagattg tatcattgtt tcctagcgtc cagaacccac aagcgaggta cgtgtactca
     2881 gctgacgtct ccgtagacga agggccgaac cagggaccgg cggaagtcgt cactgggatt
     2941 atctgggtct cctgcctcct gttcgaggcg ctcgatgaac tctggggtgg ggatcagctc
     3001 cacctccccg gagggtgccg gatctgcggc gacgtaggag atgctagcgt cggcgcggag
     3061 gagctggatg aggtcctcgg cggggagctc gaccccgtcc caattctcgt tcttttgagg
     3121 aggggcgcca agtacgcgga cggcgaagtt tatggctttt tcgcgtgagg agaagacggg
     3181 tagcaggacg ccgtcctgaa cgccgaagag gccgatgctg atcgggcccg cgtgcggccc
     3241 ttctgggtag aaggcgacga atacgaatct gggaacctgg gaagaggaag aattcatcga
     3301 accgcctcgg cgtgaggcta ggccccgttg cggacgctct gggcgcggga ctggccgaca
     3361 tcgaaatcca gtgttctccg ggcttcggtg caccggatcg tcctggccat cttcttctcc
     3421 tttcctcctc acttgatcat agcagggtgg agaaatccgc tcaacgagcc ccgcgactcg
     3481 atagaaaccc tcgtgataac atccctcaaa gccgatgcgt gacacggcgg tacgccaacg
     3541 ccgcttggcg gcgaacgcag agcagcgacg gaggtcaagg atgaaaatca acaagtggac
     3601 ccggcggcac gccaactctg aagaggacaa tgcgccgccg gaggccgtcc ccgactccgg
     3661 agaagaggtc catcagcctc cggatggtct gtccgaggct ggcattcggg aggcgttgag
     3721 ggacgtgagg gaccctgaga tcggacgcga cctcatttcg ctgaacatga tccgctcaat
     3781 ccatatcgat gcttctcatg taaccgtcgg agtagccctg actaccgctg gatgccccat
     3841 gaagcacagg atcatcacag atatcaccga tcgactcacg atcatcgatg gcgttgatga
     3901 ggtcg
//
LOCUS       DATAIR010000787         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_226630, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000787 DATAIR010000000
VERSION     DATAIR010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226630"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VHM69_18725"
     CDS             complement(<1..636)
                     /locus_tag="VHM69_18725"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEX2742482.1"
                     /translation="MSIRDLIAILPDPLRQRSLTHPSVADPYESNGRLEFLGDSVLNN
                     RVAELVFHGYPELLEGDLTRIRAHLVRKSFLAMVGRSEGIEEIIESSVINDSVIADTV
                     EAIIGAAYLIDRDLVLRTIDEIFNPSEVDTDELRDWKTLLQETLQADGLRPTYRVISK
                     QGPAHRPSFISGVSADDREVATGEGSSIKESEQAAARAALEILGIRPVPRSA"
     gene            complement(645..>1776)
                     /locus_tag="VHM69_18730"
     CDS             complement(645..>1776)
                     /locus_tag="VHM69_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX2742483.1"
                     /translation="EISLFDPSPYPSRIAGECTDFDPTDFVSKKQARRMDRFAQLGIA
                     AGIQAAESAGIVDLIRETPQRVAIVMGSGIGGLSTWEREYRTLHERGPGRVSPFLITM
                     MVPNMAAGQLAIVLGATGPSQCPVLACATGGEAIAEGLELIRRGDADVVIAGSSEAPI
                     TPLSMAGFCAIRAVSRRNDEPSRASRPFDEGRDGFVMSEGAGAVVLESEEHAERRGAE
                     PISAVAGYGRTTDAYHVTAPDEEGRGVERAMVAAFEDSGLDAEEVGHVNAHATATPTG
                     DGPETKAISRVVPHALVSGTKSMTGHSFGAVGATEGIMCALALKHKIVPPTINLENLA
                     EDCEELNYVGSEPVEAPDLKAAISNSMGFGGHNVVIAFTSVE"
BASE COUNT          296 a          583 c          557 g          340 t
ORIGIN      
        1 ggcggagcgc gggacgggac ggatacccag gatctctagc gctgcgcgtg cggcggcctg
       61 ttcgctctct ttgatggagc tgccctcgcc ggtggccacc tcgcggtcat cagcggagac
      121 cccggagata aagctgggcc ggtgcgccgg cccctgcttc gagatcacgc gataggtcgg
      181 cctcaggccg tcggcttgaa gcgtctcctg aagaagggtc ttccagtcgc gcagctcgtc
      241 tgtgtccacc tcggaggggt tgaagatctc gtctatcgtg cgaaggacca ggtctctatc
      301 tataagatac gcagccccga ttatcgcctc gaccgtgtcg gcgataaccg agtcgtttat
      361 gacggagctc tcgatgatct cctcgatgcc ctcgctccgg ccgaccatag ccaggaagga
      421 cttcctcaca aggtgggccc tgatgcgcgt gaggtcccct tcaagaagct ccgggtagcc
      481 gtgaaagacc agctcggcga cgcggttgtt caacacggaa tcgccaagaa actccagccg
      541 cccgttcgat tcgtacgggt cggcgacgct cggatgggta agcgagcgct gcctcaaagg
      601 gtccggaagg atcgctatga ggtctcttat agacatctcg ttctctattc tacacttgtg
      661 aaggcgatca cgacattgtg tccgccaaat cccatcgagt tggagatggc ggccttgaga
      721 tcaggcgcct cgacgggctc gcttccgacg tagttaagct cctcgcagtc ttcggccagg
      781 ttctccagat tgatagtcgg tgggacgatt ttgtgcttaa gcgcgagggc gcacatgatc
      841 ccttccgtgg cgccgacggc cccgaaagag tgccccgtca tggatttcgt gcccgatacc
      901 agggcgtgcg gtactaccct ggatatcgcc ttcgtctccg gcccgtcacc ggtcggcgtg
      961 gccgtcgcgt gggcgttgac gtgcccaact tcctccgcgt cgaggccgga atcctcgaac
     1021 gccgcgacca tcgcgcgctc gacgcctcgg ccttcctcgt cgggcgcggt cacgtggtag
     1081 gcgtcggtgg tacggccgta gcctgcgact gcggagatcg gttccgcgcc gcggcgctcg
     1141 gcgtgctcct cgctctccag aacgaccgcg ccggcgccct cgctcatcac gaatccgtca
     1201 cggccctcat cgaatggacg gctcgcacgg gacggctcgt cgttgcggcg gctcacggcg
     1261 cgtatcgcgc agaagcccgc catcgagagc ggcgtaatgg gggcttccga agagcccgcg
     1321 atcaccacgt ccgcgtctcc gcgccggatc agttcaagac cttcagcgat agcttcccct
     1381 ccggtggcgc aggccagtac aggacactgg ctcggccccg tcgcgccgag cacgatcgcg
     1441 agttgtccag ccgccatgtt ggggaccatc atcgtgatca ggaacgggct aaccctgcca
     1501 ggccctcgtt cgtgcagggt gcggtactcg cgctcccagg tcgagagccc tccgataccg
     1561 ctgcccatca cgatcgccac ccgttggggg gtttcacgaa tgaggtcaac tatccccgcg
     1621 ctctccgcgg cctgtatgcc ggccgcgatg ccgagctggg cgaagcggtc catcctgcga
     1681 gcctgcttct tgctgacgaa gtccgtcgga tcgaagtccg tgcactcacc cgcgatccgc
     1741 gaaggataag gcgagggatc gaagagcgaa atctcc
//
LOCUS       DATAIR010000788         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_364264, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000788 DATAIR010000000
VERSION     DATAIR010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364264"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VHM69_18735"
     CDS             <1..708
                     /locus_tag="VHM69_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2742484.1"
                     /translation="HGERLVQLTRYPKVFPVNVYLVREEDGFTLVDTGLSGSEEAYLA
                     VARELGGEIRRIALTHAHGDHTGSLDALREALPEAEVLVPAREASFLRGDLDLTPEEQ
                     RLGELKGWWKDARTEPTREISAGDRVGSLEVVAAPGHTPGQVAYFDARDRTLIAGDAF
                     QTRAGIAVSGVMRPLFPFPALATWSLSTALESARRLRTLSPSRLAVGHGPVIENPLDA
                     MDRAIEVAERKSEQRAA"
     gene            698..1276
                     /locus_tag="VHM69_18740"
     CDS             698..1276
                     /locus_tag="VHM69_18740"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEX2742485.1"
                     /translation="MPPRAGLSREAVVEAAARLADEDGLETLSLSVLASRLGIKTPSL
                     YNHVDGLGGLRRELALLGLRELGRRLGRAAIGKTKDEATFALARAYREFVKERPGLYA
                     ATVRSSRLSYPEDPAFQAVERETLETVLAVLDSYGLHGDDALHAARGFRSTVHGFATL
                     EAAGGFGIPLDLDVSFERLLHTFAAGLRKGGR"
     gene            complement(1294..2445)
                     /locus_tag="VHM69_18745"
     CDS             complement(1294..2445)
                     /locus_tag="VHM69_18745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2742486.1"
                     /translation="MQTVDLTQVPVVDNHCHGIMHDQTFDDIASWRLAFTESTDQIMP
                     RDHVATTSLYRRMVRTLAGFLGCVPEEEAVFAARVQRDGMELGGVLLRAANVETLLLD
                     TGYPPPEEVLPRDELGKLGDCRVEPMLRLETLMEDLLAEHDSLEEVKDALASALDDVR
                     DGGHVALKSIAAYRTGLDIREWSPEDAEGAFREYRRAAGDGRTRLVHKPLLDTLLHVA
                     FAQAARQEVPVQFHVGYGDADTDLLLGNPLYLRPVLGRPDYRSMPVVLLHECYPYTRQ
                     GGYLAAVYENVYLDLSYGIPLLGYGEMLSFTRQALGVAPTSKLMYSSDGIGVPELHWM
                     SAMDGRRVLGEILGELVAHGELSIPEAEAAGESVLRGNATRLYRLASNG"
     gene            complement(2517..>3998)
                     /locus_tag="VHM69_18750"
     CDS             complement(2517..>3998)
                     /locus_tag="VHM69_18750"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2742487.1"
                     /translation="LHKCKSFIVVILPVSKARTPWNRRPKHEILDASSRKRKVFSNVI
                     GGAMQGERGVRENVARPLPEGTLWVRFVFVDHAGIPKCKAVHRDGFAGRARAGVGLAK
                     GVLALDPSGALHPESGLSPVGECRLVPDLSTLTALPFARGQAMVSCDLTEPDASTPWD
                     GCPRSALKRVLGRLAERGYRSLASFETEFYVWSAEGPLDRTPYAGSFALTPAADFVAE
                     LAETLEQMGIRLEQFHAEVGHGNLELSVAEDECLAAADRRVMVLEAIRGVAYGMGLKT
                     TMAPKPYLDAAGNGHHLHVSLYEDNSPVLFDSSGALSGQGSGFVAGLLEHLPAIMAFT
                     APSPNSYQRIAPGMWSSAFGAYGLDNREAAVRLASPVAGRESATANVELKPVDVTCNP
                     YLALAAVLAAGMDGVGRGLDPGEPTMVDPATLGDGERASRGIRPLPASLDEALDALEE
                     DTVLIEAFGEPLVRTHVAVARAQAAMARELPPEEVAAAAAMVH"
BASE COUNT          657 a         1337 c         1311 g          693 t
ORIGIN      
        1 catggagagc gtctggtaca actgacgcgc taccccaaag tatttccggt aaacgtctat
       61 ctggtgcgtg aggaggacgg gttcacgctg gtggacacgg gcctctcggg gagtgaggag
      121 gcgtacctgg cggtggcgcg ggagctggga ggtgagatcc ggcgcatcgc gctgacccac
      181 gctcacggcg accataccgg gtcccttgac gccctgcgcg aggccctgcc tgaggcggaa
      241 gtattggttc ctgcgcgaga ggccagcttc ctgcggggag acctggatct cacaccagaa
      301 gagcaacggt tgggggagct caagggctgg tggaaagatg ccaggacaga gccaaccagg
      361 gagatctctg ccggtgaccg cgtgggctca ctggaggtcg tggccgcgcc ggggcacacg
      421 ccgggacagg tggcgtactt cgacgccagg gacaggacac tgatcgccgg cgacgccttc
      481 cagacccgcg ccggcatcgc cgtatcgggt gtgatgcgtc ccctcttccc gttccctgcc
      541 cttgctacct ggtccttatc caccgctctc gaaagcgcca ggaggctgcg gacgctatcg
      601 ccctcacgcc tggcggtcgg tcacggccct gtcatcgaga acccactaga tgcgatggac
      661 cgcgccatcg aagtcgcgga acgcaagagc gagcagcgtg ccgcctaggg cggggttgag
      721 ccgggaggcg gtggtggagg ccgccgccag gctcgcggac gaagacggtc tcgaaacgct
      781 ctctctttcg gtcctggctt ctaggctcgg gataaagacg ccgtcgctgt acaaccacgt
      841 cgacggcctc gggggactgc gccgcgagct cgcgctgctc ggcctgcgcg aactaggccg
      901 caggctcggt cgagccgcca tcggcaagac caaagacgag gccaccttcg cactagcccg
      961 agcctaccgc gagttcgtaa aagagcgccc tgggctctac gccgcgacgg tcaggtcgtc
     1021 ccggctctcc taccccgaag atccagcctt ccaggccgtc gagagggaga ctctggagac
     1081 ggtgctggcc gtgctcgact cctacggcct tcacggcgac gatgcgttgc acgccgcgcg
     1141 cgggttccgt agtacggtgc acgggttcgc cacgctcgag gcggccggcg ggttcggtat
     1201 acccctggat cttgacgtga gcttcgaacg gctgctccac accttcgccg ctggactcag
     1261 gaaaggtgga cggtaaaggg tttgcggtcg tggttacccg ttggacgcca gccggtacag
     1321 gcgcgtcgcg ttcccgcgca gcacactctc gccggccgct tcggcctcgg ggatgctgag
     1381 ttctccatgt gcgaccagct ctccgagtat ctctccgagc acacgccgcc cgtccatggc
     1441 gctcatccag tgcagctcgg gcacgcctat gccgtcggag ctgtacatga gcttggaggt
     1501 cggggcgacg ccgagcgcct gccgcgtaaa ggagagcatc tcgccgtagc ctagcagagg
     1561 gatgccgtat gagaggtcga ggtagacgtt ctcgtatacg gcggccaggt agccgccctg
     1621 cctggtatac gggtagcatt cgtgcagcaa caccacgggc atgctccggt agtcgggccg
     1681 ccccagcaca ggtcgcaggt agagtggatt gccgagcagg aggtccgtgt ctgcgtcgcc
     1741 gtagccgacg tggaactgga cagggacctc ctgcctggcc gcctgcgcga acgcgacgtg
     1801 cagcagggta tccaggagcg gcttgtggac gaggcgcgtc cggccatctc cggcggcccg
     1861 ccggtactcg cggaacgccc cttccgcatc ttccggggac cactctcgga tgtccagacc
     1921 cgtcctgtag gctgcgatgc tcttgagcgc cacgtggccg ccgtctcgca cgtcgtcgag
     1981 ggcagatgcc agtgcgtctt tcacttcctc gagagaatcg tgttcagcca gcaagtcctc
     2041 catcagcgtc tcgagacgca gcatcggctc gacgcggcaa tctcccagct tccccaattc
     2101 atccctcggg agcacttctt cgggcggcgg gtatccggtg tcgaggagca gggtctcgac
     2161 gttggcggcg cgcaggagaa caccgccaag ttccattcca tcacgctgca cgcgggcggc
     2221 gaagaccgct tcctcttccg ggacgcatcc gaggaagccg gcgagagttc ggaccatgcg
     2281 ccggtataaa gacgtcgtgg cgacgtggtc gcgcggcatg atctggtccg tggactccgt
     2341 aaaggcgagc cgccagcttg cgatgtcatc gaatgtctgg tcgtgcatta tcccgtggca
     2401 gtggttgtcc acgacgggga cctgcgtgag gtctaccgtt tgcatgcgcc acctccttac
     2461 agccagcagg aatcaggctt cagatatttt cctgatgcct gtgacctgat gcctatctag
     2521 tggaccatcg ctgccgctgc cgcaacctcc tcgggtggaa gctccctggc catggcggct
     2581 tgcgcccgcg ccacggccac atgggtgcgc acgagcggct cgccgaacgc ctctatgagg
     2641 accgtgtctt cctccagcgc gtccagggct tcgtccagcg aggcaggcag cggcctgata
     2701 cccctggagg cgcgctcccc gtcgcccagc gtggcggggt ccaccatcgt cggctccccc
     2761 gggtcgaggc cgcgcccgac cccgtccatc ccggcggcca ataccgctgc cagtgccaga
     2821 tagggattgc aggtgacgtc gacgggcttg agctcgacgt tggccgtcgc tgactccctg
     2881 cctgcaaccg gcgaggcgag gcgcacggct gcctccctgt tgtccaggcc gtacgcgccg
     2941 aacgccgagg accacatgcc gggggcgata cgctggtacg agttcgggga aggggccgtg
     3001 aacgccatga tggccggcag gtgctcgagt aacccggcca cgaagccgga gccctgtccg
     3061 ctaagggccc ccgaggagtc gaagaggacc ggggagttgt cttcgtagag gctaacgtgc
     3121 aggtggtggc cgttgcccgc cgcgtcaagg tacggtttcg gggccatcgt ggtcttcagt
     3181 cccataccgt aggccacgcc ccggatcgcc tctagcacca tcacccgcct gtccgcggcc
     3241 gcaaggcatt cgtcctccgc cacggagagc tccaggttcc cgtgcccgac ctcggcgtgg
     3301 aactgttcca gccttatgcc catctgctcc agggtctccg caagctctgc gacgaagtca
     3361 gccgccgggg tcagcgcgaa gctccccgcg tacggcgtcc tgtccagggg accttcggcg
     3421 ctccagacgt aaaactcggt ctcgaaggag gcgagcgacc tgtaacctct ttcggcgagc
     3481 ctcccgagca cccgcttgag ggcgctgcgg gggcatccat cccagggcgt actggcgtcg
     3541 ggctccgtca agtcgcagga gaccatggcc tgcccgcggg cgaatggcag ggccgtgagg
     3601 gtcgagaggt cagggaccag ccggcactct cccaccgggc tcaatcccga ctcggggtga
     3661 agagccccgc tcgggtcgag ggccagcacc cccttcgcga gccccacccc ggctctcgcc
     3721 ctccccgcga agccgtccct gtggaccgcc ttgcacttgg ggatgccagc gtgatccacg
     3781 aacacgaacc ggacccacag agtaccctcg ggcaaaggcc gcgccacgtt ctcccgcacc
     3841 ccacgctcgc cttgcatagc accccctatg acgttgctga agacctttct cttccgcgat
     3901 gatgcgtcca atatctcatg cttcggtctc ctgttccacg gtgttcgggc cttacttaca
     3961 gggaggatca caacgatgaa ggacttgcat ttatggag
//
LOCUS       DATAIR010000789         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_271116, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000789 DATAIR010000000
VERSION     DATAIR010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271116"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..211)
                     /locus_tag="VHM69_18755"
     tRNA            complement(139..211)
                     /locus_tag="VHM69_18755"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Lys,seq:ctt)
     gene            complement(410..1741)
                     /locus_tag="VHM69_18760"
     CDS             complement(410..1741)
                     /locus_tag="VHM69_18760"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742488.1"
                     /translation="MSGTVGELFGGSGRRVRDVIWGDVSVDRAVKALLETGAMERLKG
                     MSQLGFTFGSFPRARHTRMDHAIGVYHLAGLTLKRITEQGAYLEDREVRGALAAALLL
                     DAGRYPYANSIEGIHLPGAPTLKELSRRWIEHTEVAKILRSEWDLEPHNVFRLVSRGD
                     TPPHSLTPTEHLLRDILFGALNVDALDRLIRDARGARVPYGLVEVESLIERLRIVGQE
                     NRAVLALDEDGAGTLQSLVFSRYLVYYNVYGHHTLRIPTVMFLRAVQDAIQAGTMTFD
                     ELSEQDDAGAFVLLEDSAEPESSTAALMKRLADRRPYHLALEFDERHPSYSSLIRLRD
                     DASWRRRVEEAWSRYLTRYRKGVAGPFDILIDLPEKEHFPIDLRLIRRTPLPGERNPV
                     GWHHLSGMSEDEVTRYHAPLRRIIIATANDDLVKSVRRHAEELFTIAEEVG"
     gene            1817..2377
                     /locus_tag="VHM69_18765"
     CDS             1817..2377
                     /locus_tag="VHM69_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742489.1"
                     /translation="MHFREGDAIDTEELEVIGGESVILRPPRESDVEAVYSWDRDPEL
                     AAWNGRSPISISLAAARRDYLARWEDRSVKTFIIEAAEEAIGMATLYDFRHDGCELGI
                     KIGPGNLRGRGYATEAVTLLLDYVFNTLGLGTVRGSTLAHNDRMQRVFEKNGFEQVGD
                     GSILSRYDNRRYTELFYERRREDPGP"
     gene            complement(2387..3010)
                     /locus_tag="VHM69_18770"
     CDS             complement(2387..3010)
                     /locus_tag="VHM69_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742490.1"
                     /translation="MRLATLAVVVMLFLFSSAAAFAVTAGCVVGKICYGTRIDDRLTG
                     TNEDDEVLARGGRDFVNARGGSDVVHGGSGADGGEFGRGGLMGDSPGMSANSNRDGDD
                     IIHGGDGRDTMYGFGGDDLLVGGQSSDYIFAEEFRTRFGRPGFARSKNPGKDTVSSGG
                     GGDHIEAVDGRRDVIACGGGKDAVWFDEGLDTVASDCELKNVIHSGG"
     gene            3219..4133
                     /locus_tag="VHM69_18775"
     CDS             3219..4133
                     /locus_tag="VHM69_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742491.1"
                     /translation="MRTQLELNIRSRGAMSGVLTMGSLDPAFAAATRVEGEPVMVDLR
                     SVEFVEPAGLCGLAALLEYLSARSEEVDLALAGRDVPAYLERMNFFRSFGDRVRTNAD
                     VASLEERHRHNPGTLQELINFHAEEEIPGIIERISEILENQGYRLRERVAICSTLSEV
                     CANAAEHGVSSFGAYAAVQAYHHIVSGSRRRGEEVLIAIADGGVGVRETLSRNPKYAS
                     HTTTDNDALRHALEMGVSATGEIGRGGGLALVAGIAARASGSLSIRSGSGRITVYENR
                     KNSRNVPEFPGTFVRVSLPRTPQEKASK"
     gene            4229..4801
                     /locus_tag="VHM69_18780"
     CDS             4229..4801
                     /locus_tag="VHM69_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2742492.1"
                     /translation="MIEERAHGVFEVSGGDFGGKLMVTRRTGRAVREALEAALEELPK
                     GGILYVDTRGVELMDYSFADEALGILASRSASGEYEGRHLVLVEEDRDLLENVEASLR
                     QRSLAMIRVDEIGAEPGIVGKVPDHLVETLQAIYDAGSITNAALAARLGLNHTACNNR
                     ATRLAKLGLIHRRTETAAPGGRQYTYERIA"
BASE COUNT          942 a         1498 c         1561 g          945 t
ORIGIN      
        1 tagtgcgcct tctcttgttg ctcattggta tctccccacg agatagtctg tcagataccc
       61 tgtcaaaccc aggtagggga gtgggggagc cctagaaata gaaaacggat ttccgctgta
      121 taagcgagaa atccgaagtg agccgcccag gactcgaacc tggaacctag agattaagag
      181 tctcttgctc tgccaattga gctagcggcc catgagggca gattatatca tcgctcgcgg
      241 gcaaatagtc accaaggtta agttttgttc acagggcttg cgcctgaggc ggaggcacaa
      301 ttataatctt agtatcatcc tagcggtcct atctgggggc cgctaagggc cggaaaaggg
      361 tctcaccctt atatgaacac cggccctttt tctatgcgcg gtcgtcgtgt catcctactt
      421 cctcggcgat ggtgaagagt tcttcggcgt gacggcgcac ggacttgacg aggtcgtcgt
      481 tggccgtggc gataatgata cgccgcaggg gggcatggta gcgcgtcacc tcgtcctctg
      541 acatgcctga gaggtgatgc cagcccacgg gattacgctc gccggggagg ggggtgcgtc
      601 ggatcaggcg caggtcgatc gggaagtgct ctttttcggg gaggtctatg aggatgtcga
      661 agggaccggc gacgcccttg cggtagcggg tgaggtagcg ggaccaggcc tcttccacgc
      721 ggcggcgcca ggaagcgtcg tcgcggaggc gtatcaggga ggagtagctc gggtggcgct
      781 cgtcgaactc gagggcgaga tggtagggcc tgcggtcggc gaggcgtttc atgagggcgg
      841 ccgtggagct ctcgggctcg gcagagtctt caagcaggac gaaggctccc gcgtcgtcct
      901 gctcggagag ctcgtcgaag gtcatggtgc cagcctgtat ggcgtcctgg acggccctga
      961 ggaacatgac ggtcgggatg cgcagggtgt ggtgaccgta gacgttgtag tacacgaggt
     1021 agcgtgagaa gaccagggat tgaagggtgc ctgccccgtc ttcgtccagc gccagtacgg
     1081 ccctgttctc ctggcccacg atcctgagac gttcgatgag tgactccacc tcgacgagcc
     1141 cgtaggggac cctggctccc ctggcgtcac ggatcaggcg gtccagcgca tccacgttca
     1201 gggcgccgaa gaggatgtcc ctcaggaggt gttcggtggg cgtcaggctg tgtggcggcg
     1261 tatctccgcg tgagaccagc ctgaagacgt tgtggggttc aaggtcccac tcgctacgca
     1321 ggatcttcgc cacctcggtg tgttcgatcc aacgccggct caactccttg agggtgggcg
     1381 cccctgggag gtggatgcct tcgatggagt tggcgtaagg gtatctgcct gcgtccagga
     1441 gcagtgccgc ggcgagggcg ccccttacct cacggtcctc cagataagcg ccttgctcag
     1501 tgatgcgctt cagcgtcagc ccggcgagat ggtagacgcc gatggcgtgg tccatgcgcg
     1561 tatggcgggc cctagggaag gacccgaaag tgaatccgag ctggctcatg cccttcagac
     1621 gctccatcgc gcctgtctcg agcaaggcct tcaccgcccg gtctacggag acgtcccccc
     1681 agatcacatc cctgactctt ctgccggagc ccccgaagag ctccccaacc gtaccgctca
     1741 atgacgctcc cgatcttctg cttcgcgcta ttctaacgaa aatggactcg cccgaaagga
     1801 tgcttctggt gtaacattgc attttcggga aggagacgcc atagacacag aagagctgga
     1861 ggtcatcggg ggggagtcgg tgatcttgag gccgccgcgg gaatcggacg tggaggcggt
     1921 ctattcctgg gacagggacc ccgagctcgc cgcctggaat ggtcgctccc ctatctccat
     1981 ctccttagcg gcggcccgcc gcgactacct ggcacgctgg gaggaccgga gcgtcaagac
     2041 ttttataata gaggccgcgg aggaagccat cggcatggct accctctacg acttccgcca
     2101 cgacggctgc gaacttggca taaagatcgg acccggaaac ctgcgcggca ggggctacgc
     2161 cacagaagcc gtgaccctcc tcctcgacta cgtattcaac accctgggcc tcgggaccgt
     2221 tcgcggctcg accctggccc ataacgaccg catgcagcgc gtcttcgaga agaacggttt
     2281 cgaacaggtc ggggacggct ccatcctgag ccggtacgac aaccgccgct acaccgagct
     2341 cttctacgaa cgccgtcggg aggatccggg cccgtaaggc agacttttac ccgcctgagt
     2401 ggatgacgtt ctttagctcg cagtcggagg ctacggtatc tagtccctcg tcaaaccaga
     2461 ccgcgtcctt gccaccgccg catgcgatga catccctgcg accgtctacg gcctcgatgt
     2521 gatctccacc acctcctgag ctcacggtgt ctttgccagg gttcttcgag cgcgcgaatc
     2581 cagggcgacc gaacctggtg cggaactctt cggcgaagat gtagtcgctg ctttggcctc
     2641 cgacgagcag gtcatcgccg ccgaaaccgt acatggtgtc gcggccatcg ccgccgtgga
     2701 tgatgtcgtc accatctctg ttgctgtttg cggacatgcc aggggaatcg cccatgagac
     2761 cgccgcggcc gaactcaccc ccgtcggccc ctgaaccgcc gtgcacgacg tccgagccgc
     2821 cacgggcgtt aacgaagtcc cgcccgcccc tggcaaggac ctcgtcgtcc tcgttggttc
     2881 ccgtgagacg gtcgtctatc ctggtcccgt agcatatctt gccgacgaca caacccgccg
     2941 taaccgcgaa ggccgcagcc gaagagaaca aaaagagcat caccaccacc gctagagtcg
     3001 ccaaccgcat gaccttctcc tctcgtcata ccgctcggcc cctccagcgc tatgggacca
     3061 ctctatgaca ttcgcgttac gagatcgcaa cgaccgtgta acgcttctcc tgcgttttga
     3121 taacgtttcg ataactgcgc gcagaagttg ccggtctcgg agtatgtttg cacgccgtcc
     3181 ctctcgctat cctgccctgg ccgactacaa taaggctgat gaggacacag ctcgaactga
     3241 acatcaggag caggggcgcg atgagcgggg tgctgaccat gggcagcctg gaccctgctt
     3301 tcgcggccgc cacgcgggtt gaaggagagc ctgtgatggt cgatctgcgt tcggtcgagt
     3361 ttgtcgagcc ggccggtctg tgtggtctcg ccgcattact ggaatacctg agcgcgcgca
     3421 gcgaagaggt cgacctggcg ctcgcgggac gggacgtgcc cgcctacctg gagcggatga
     3481 acttcttcag gtcgttcggg gatagggtgc gtaccaacgc cgacgtggcc tcactggagg
     3541 agcgccatcg gcacaacccg gggacccttc aggagttgat caacttccac gcggaagagg
     3601 agataccggg gatcatcgag aggatatcgg agatcctgga gaaccagggc taccgcctgc
     3661 gggaacgcgt cgccatctgc tctacgctct ccgaggtctg cgcgaacgcg gccgagcacg
     3721 gcgtctcctc tttcggggcc tacgccgccg tccaggcgta ccatcatatc gtcagcgggt
     3781 cccgccgccg cggcgaagaa gtcctcatag ccatcgccga tggcggcgtg ggcgtgcgcg
     3841 agacgctctc ccgcaacccg aagtacgcct cccatacaac cacggacaac gacgccctgc
     3901 gccacgcgct cgagatgggc gtctccgcga caggggagat cggacgcgga ggaggcctcg
     3961 ctctcgtcgc aggtatagcc gcccgcgcca gcggatcact ctctattcgc tcgggctcag
     4021 gccgtatcac cgtctacgaa aaccgtaaaa actcccgtaa cgtccccgaa ttccccggta
     4081 ccttcgtccg cgtctccctg ccgcgtaccc cgcaagaaaa agcctccaaa tgaaggcaaa
     4141 atatgggccg atatgctgtt gacttatcta catatagccg gtacaatcca tttatatgat
     4201 ttcactgacg acagtgagga cagctccggt gattgaggag cgggcgcacg gggtcttcga
     4261 ggtctctgga ggcgacttcg ggggcaagct catggtgacc cgcaggacgg ggcgtgctgt
     4321 tcgtgaggcg ctcgaagcgg cgctggagga attaccgaaa ggtggcatcc tctatgtgga
     4381 cacgcggggg gtcgagctca tggactactc gttcgcggac gaggcgttgg ggatcctggc
     4441 gtcgaggtct gcgagtggag agtatgaagg ccggcatctc gtcttggtcg aggaggacag
     4501 ggatctcctg gagaacgtcg aggcttcgct ccggcagcgg tctctagcca tgatccgggt
     4561 agatgagatc ggggccgagc cgggcatagt cggcaaggtg cccgaccatc tcgtcgagac
     4621 gctgcaggcg atctacgacg caggctccat caccaacgcg gccctcgcgg ccaggctcgg
     4681 tctcaaccat acggcctgca acaacagggc gaccaggctg gcgaaactcg gcctgatcca
     4741 ccgccgcaca gagaccgcag ccccaggcgg acgccagtat acctacgagc gtatagcctg
     4801 atgtttattt ttgaccacat cctcaccagg ttcagtgaag atagcgtcac cggcattttg
     4861 ctggaggagg ttcagtcgtg agggacgagt acatgatcga gcgccagggc aagaggttcg
     4921 tgctctatgc ggggcttctt gaggag
//
LOCUS       DATAIR010000790         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_227077, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000790 DATAIR010000000
VERSION     DATAIR010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227077"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..545
                     /locus_tag="VHM69_18785"
     CDS             99..545
                     /locus_tag="VHM69_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742493.1"
                     /translation="MHLCIGLNRNHHISTCRKTAGGGGYAINLGADANTLQIEGKENL
                     SVYHAKLYDRGEPGNTLISEAERNFCKVCGSFLWLYDPRWPDLVHPFASAVDTSLPKP
                     PERVHVALEFAAPWCMVPEGENERRFALWPEESIAQWHRRHGLERP"
     gene            599..1006
                     /locus_tag="VHM69_18790"
     CDS             599..1006
                     /locus_tag="VHM69_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2742494.1"
                     /translation="MQSFYREIVLDHFQHPRNRRVLEDADIEEYIDNPLCGDVVTVYA
                     NLHDSIFEEVSFTGRGCSISQASASMLTEQLTGKSRDEAQEKIETFLEMMRTEEKEEL
                     GELAALKGIVQTPNRIRCATLAWDAMRRALSGL"
     gene            1120..2616
                     /locus_tag="VHM69_18795"
     CDS             1120..2616
                     /locus_tag="VHM69_18795"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX2742495.1"
                     /translation="MRRPGLEEGGSAERPISGGFRHEGLDRALARASDASLREGNRLE
                     ILKNGPETYDDWLQAISGARRWVHLDNYIFKDDEVGARFAEALIFKSEEGVSVRVLYD
                     WFGCLDVRASFWLRMREAGVEVRVVNPPTLGGPLGTIRRDHRKLLAVDGEYASTGGVC
                     ISEGWLVTSPETGLPYRDTAVGVRGPAVADVERAFAGLWSEAGGELPQEERPEPVNMP
                     RVGEQAARVVVQEPRRMRTLRMLQLLTGGVQRRLWITDAYFLSMAILSQSLMSVARDG
                     VDVRVLVPATNDLPWIGALSRTGYRQFLESGVRIFEYGGPMIHAKTLVADGWWSKVGS
                     TNLNFASLVANWEIDLVAEDTGFAAQMEALFEEDLSHAREVLLAGTAAHPRVRPERRI
                     QTSDREARRGAVGSGTGGSATVLRVGTTALQKSGYPLETHEQALAAAASGGLLAASVL
                     SLRFPRLLAWPLAAAGTIFGGLGVLRAARSALSDRRQRSSAPQDTDDS"
     gene            2692..3453
                     /locus_tag="VHM69_18800"
     CDS             2692..3453
                     /locus_tag="VHM69_18800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2742496.1"
                     /translation="MSPVAIVTGSDSGIGKESAKELAENGFDLGITYHTDEAGANETL
                     EAVRAVGRRGEVRRLDLTELPGAADVIDDLADSLGGVDVLVNNAGTGDPTREFLKLPY
                     EEWRQVIDTNLSGAFLCAQRAARRMVDAGNGGRIINVTSVHEQIPRTGASAYCSSKGG
                     LGLLTKVMAMELAEYGILVNAVGPGEISTPMTGAEDEDPTTIERPNLPLGRPGHAREI
                     AAWVAFLASEKSSYATGSSFVVDGGLTLMAAELSS"
     gene            complement(3502..>3935)
                     /locus_tag="VHM69_18805"
     CDS             complement(3502..>3935)
                     /locus_tag="VHM69_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742497.1"
                     /translation="PCVTMTSSMETPRKMPRLFRGPPKTLFDDAGDEAFEAPAKGPLF
                     SSSVFYALLAFFGVSLMLTADWFGLLLLNSEGVFERIGPLNSVRVTAVLMTGIAIPSA
                     LLMFHYKRLYSYTSDENVFFAITFSLSFLLGFPCALAGIFG"
BASE COUNT          750 a         1189 c         1326 g          670 t
ORIGIN      
        1 cgtgttactt gaagtctcgt gccactgcgt ggcggtgaac ttccgggtgg agtcggagac
       61 gccgtaccct tatcaggctt gctactgaaa tgtgatggtt gcatctgtgt attggtctca
      121 atcgcaacca tcacatttcc acctgtcgca agacggcggg cggaggaggg tatgcgatca
      181 acctcggcgc ggacgcgaac accctgcaga tcgaaggcaa ggagaacctg tccgtctacc
      241 acgcgaagct ctacgaccgt ggcgagccgg gtaatacact gatcagtgag gcggaacgaa
      301 acttctgcaa ggtctgcggg agcttcctct ggctctatga tccgcgttgg cccgacctcg
      361 tccatccttt cgcctcggcc gtagacacct cgttgccgaa gccgccggag cgggtacacg
      421 tcgcgctgga attcgccgcg ccatggtgca tggtccccga gggcgagaac gagaggcgct
      481 tcgccctgtg gcccgaagag tccatagctc agtggcaccg tcgccacgga ctcgaacgcc
      541 cttagggacc tcttccggcg aagaacctca cgctgctgat acaatttctt gcagggtgat
      601 gcagagcttt tacagagaga tcgtcctcga ccatttccag cacccgcgca accggcgcgt
      661 gctggaggac gcggacatcg aggagtacat cgacaacccc ctctgcggcg acgtggttac
      721 ggtctacgcg aacttgcatg acagcatctt tgaagaggtg agctttaccg gacgcggatg
      781 ctcgatctcg caggcgtccg cttcgatgct tacggagcaa ctcacgggca agagccgcga
      841 cgaggcgcag gagaagatag aaacttttct ggagatgatg cgcacggagg agaaggaaga
      901 gctaggggag ctcgccgccc tcaaaggcat tgtgcagact cctaaccgca tccggtgcgc
      961 caccctcgca tgggatgcta tgaggcgtgc cctctcagga ctgtaggcca gaaagtctcg
     1021 cttggaggta ccggaggtcg cgttcaacca ggcgcataga ggctctgtcc tgccgtcgac
     1081 gaatacgtga tgacagggta gagtaaagtt tcacccttcg tgaggaggcc gggactggaa
     1141 gagggaggat ccgcggaacg accgatttcg ggcgggttcc ggcacgaggg actcgaccgt
     1201 gccctggcgc gagcaagcga tgcgtcgctc agggagggca accggttgga gatcctcaag
     1261 aacggtcccg agacctatga cgactggctt caagctatct ccggggcgcg gcgctgggtg
     1321 cacctggaca actacatctt caaggacgac gaggtcggag cgaggttcgc tgaggctttg
     1381 atctttaagt ccgaagaagg cgtgagcgtc cgagtgctct acgactggtt cggttgcctg
     1441 gacgtgcggg cctccttctg gctgcggatg cgggaggcag gcgtggaagt cagggtggtc
     1501 aacccgccga cgctgggcgg cccgctcggc acgatccgac gcgaccaccg caagctgctt
     1561 gccgtggacg gtgagtatgc ctcgacgggc ggcgtgtgca tctccgaagg ctggctcgtg
     1621 acctcccccg agacggggct cccttacagg gacacggccg tcggcgtcag aggcccggcc
     1681 gtggccgacg ttgagcgggc cttcgccgga ctgtggagtg aagccggggg ggaactcccc
     1741 caggaggaac gccccgagcc agtaaacatg ccgagggtcg gcgagcaggc ggcgcgggtc
     1801 gtcgtccagg aaccgcgcag gatgcggacc ctcaggatgc tgcagttgct caccggcgga
     1861 gtacagcggc ggctttggat caccgacgcc tacttcctct ccatggcgat cctctcccag
     1921 tccctgatgt ccgtggcgag ggacggtgtg gacgtgcggg tcctcgtgcc ggcgaccaac
     1981 gacctgccct ggatcggggc gctctcacgc acgggctaca ggcagttcct ggaatcgggg
     2041 gtgcgcatct tcgagtacgg cggcccgatg atccacgcca agaccctcgt cgcggacggc
     2101 tggtggtcga aggtcgggtc gacgaacctc aacttcgcca gcctcgtcgc caactgggag
     2161 atagaccttg tggccgagga caccggcttc gccgcccaga tggaggcgct gttcgaagaa
     2221 gatctctccc atgcccgcga ggttctcctc gccgggacgg ccgcgcaccc gagggtgcgt
     2281 ccggagcgcc gcatccagac ctcggatcgt gaggcccgaa ggggcgcggt cggaagcggc
     2341 acgggcgggt cagccaccgt ccttcgggtc gggaccacag ccctccagaa gagcggctat
     2401 ccgctggaga cgcacgaaca ggccctggcc gcagcggcga gcggcggcct gctcgctgcc
     2461 tccgtcttga gtctccgctt cccgcgcctc ctcgcctggc ctctcgccgc tgccggcacc
     2521 atattcgggg gcctcggggt gctgcgcgcc gcccgctcag ccctctccga tcggaggcag
     2581 agatcttccg ctcctcagga caccgacgac tcctgatcca cactccgttt gtgctctcag
     2641 gtccaggggc atagagccgg gtaagcgctt ttccgaacga gaggagagag catgagcccg
     2701 gtagccatag ttacaggatc ggactcgggg atcgggaagg agtcggcgaa ggaactggcg
     2761 gagaacgggt tcgacctcgg catcacctat cataccgacg aggcaggggc caacgagacg
     2821 ctcgaggccg tgcgggctgt gggccgcagg ggcgaggttc ggcggctgga cctgaccgag
     2881 ttacctgggg cggcggatgt gatagacgac ctggccgact ctctgggcgg ggtggacgtg
     2941 cttgtcaaca acgccggcac tggcgacccg acgcgcgagt tcctcaagct cccctacgag
     3001 gagtggcgtc aggtcataga caccaacctc tcgggggctt tcctgtgcgc ccaacgggcg
     3061 gcgaggagga tggtcgatgc ggggaacgga ggcaggatca tcaacgtcac ctcggttcac
     3121 gagcaaatcc cgcgcacagg cgcctccgcg tactgctcct ccaagggcgg tctcgggctc
     3181 ctgacaaagg tcatggcgat ggaactggcg gagtacggca tcctggtcaa tgccgttggc
     3241 cctggtgaga tctctactcc catgaccggc gccgaggacg aggacccgac gactatcgag
     3301 cgcccgaacc tgccactcgg acgtcccgga cacgcacgtg agatcgccgc ctgggtggcc
     3361 ttcctcgcct cggagaagtc ttcctacgcc acaggctcct ctttcgtcgt ggacggcggc
     3421 ctgacattaa tggctgcaga attgtcgagc taaggcggtt tgcggaaggt cacttccacg
     3481 aaccgcgccg tcgggcgtca gctatccgaa tatacccgcg agggcgcagg ggaagccgag
     3541 gagaaaagag agcgagaagg tgatggcgaa gaagacgttc tcgtccgagg tgtaggagta
     3601 gaggcgcttg tagtggaaca tcaggagggc cgaggggatg gcgatccccg tcatcaggac
     3661 ggccgtcacg cgcacggaat tgagaggccc gatccgttcg aagacgcctt ctgagttgag
     3721 gagcaaaagc ccgaaccagt cggcggtaag catgagggag acaccgaaga aggcaagtag
     3781 tgcgtagaac acggaactcg aaaacagcgg acccttcgcc ggcgcctcga aggcctcgtc
     3841 cccagcgtcg tcgaacagcg tcttcggcgg tccacggaag agcctcggca tttttctggg
     3901 ggtctccata gaggacgtca tggtaacgca aggag
//
LOCUS       DATAIR010000791         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_169305, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000791 DATAIR010000000
VERSION     DATAIR010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169305"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VHM69_18810"
     CDS             complement(<1..339)
                     /locus_tag="VHM69_18810"
                     /inference="COORDINATES: protein motif:HMM:NF024980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2742498.1"
                     /translation="MVGYSKKWTTEIVRRYESGGVEALGDRRHSNPGAKERALLDEAG
                     EEELLEALAGPPPGGGMWSGPKVARWIAQRNGLEKVHVQRGFEYLRKLRYSPQIPRPE
                     NASADASEQEA"
     gene            complement(546..1041)
                     /locus_tag="VHM69_18815"
                     /pseudo
     CDS             complement(546..1041)
                     /locus_tag="VHM69_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1343..1528
                     /locus_tag="VHM69_18820"
     CDS             1343..1528
                     /locus_tag="VHM69_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742499.1"
                     /translation="MHRHAAENIAQADALLFGRVTYEMMEAAWRPPARTGVRPDWMAD
                     WMEPFARTIDAAKKYVV"
     gene            1565..1828
                     /locus_tag="VHM69_18825"
     CDS             1565..1828
                     /locus_tag="VHM69_18825"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2742500.1"
                     /translation="MRGDHLGNAVQQLKRESGKGLFVGGVKLPLALAELGLIDEYEFV
                     VQPRLAGHGPTLFAGLSKHIDLKLVSRLEFGSGAVAMRYEPRR"
     gene            complement(1987..3225)
                     /locus_tag="VHM69_18830"
     CDS             complement(1987..3225)
                     /locus_tag="VHM69_18830"
                     /inference="COORDINATES: protein motif:HMM:NF021207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HEX2742501.1"
                     /translation="MSDLSAKALIMVVSRTDAVEVAVAELGPEIVGVILSQDILGPIV
                     MKCQEMEDRATFRYRIVDSPMEIGDSFERFEHLLSEIEGLGYATGDVVLDVTGGTTPM
                     RFGAALAAMTRGMRMVHQRVPQRFVEGEWILDASRQREIVPMDNPLESTGLLREGQAV
                     ELFNRRDYGAAALVFEDVARKVTGVERGHYYNGLLLLAEGYGAWDVADYGTALEKLKL
                     AREELGVGFSEAALAERAGEITGRITAHLPFLGKVRGKLSVENVVDMVENARRRITDQ
                     GRYDDGVARLYRAVEMWHQWRLMSFYSIETKKVKWERVDKDARDHFLKLVQTKELPDG
                     LGLRHARILDHILSGKDPEEEDTAFQNLLTARNSSILAHGVDPIGQGTAKRFLMYLDT
                     LVTVPEDLRSGARHADLLAL"
     gene            complement(3222..>4226)
                     /locus_tag="VHM69_18835"
     CDS             complement(3222..>4226)
                     /locus_tag="VHM69_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2742502.1"
                     /translation="PGAFWDLILDAPQDCDISFAPEPEVPESNPNVQETGELEKGPPD
                     PWPIEATADGDDAQIAAQLGVEEWEDEYFALRRDVENIPTRPRPRHILPPPETHSPSS
                     LMDLETCARRYYYTYVFPVPLGSERMEESQEYGSAVHAWIEGGMQGDPPKPEAAGGNG
                     IGRAPAGDFSTSEYALKASSYPLHEGTDLPETGPARMVEVSFAVEIGGAEVRGRIDAV
                     FLDEDGTVHLIDWKTGRPHDSYKSRLQLPLYALAANRLWGVEPERMRLAYVFVPGDER
                     LEVEMLEGFLERAEERVIKALETIRSGAYEPTPSHHACSYCPVMGVVIEGCPPEVPEK
                     "
BASE COUNT          743 a         1313 c         1291 g          879 t
ORIGIN      
        1 agcctcctgc tcggaggcat cggccgaagc attctcgggc ctcggaatct gtgggctgta
       61 tctgagcttc ctgaggtact cgaaaccccg ctgaacgtgc accttctcta acccgttcct
      121 ctgcgctatc cagcgtgcca ccttcggtcc gctccacatc ccgccacccg gaggaggtcc
      181 cgcaagggcc tccagtagct cctcctctcc ggcctcgtcg agcagcgccc tctctttggc
      241 accagggttg gagtggcgac ggtcgccaag agcctcaacc cctcctgact cataacgcct
      301 tacgatctcg gtggtccact tcttggagta acccaccatc tcagccacct cgccgagcga
      361 atccccaagc gacactcgcc agaccatcaa caggtgcgtg cgcagtaccg gatctgttgc
      421 ctttcggtag agactctcta accggcggga ctccgatagc tctgaatttc gtttctcgtc
      481 catgtctcga agtatacagc cggattccgt atcagaggaa gatgggagaa agttagggcc
      541 tgtcgtcatt tgagccaagc cagggcgcag acgaggtgta cgcctgcgag gaagtttctg
      601 gcggtctttt cgtagcgggt ggcgatggcg cgaaagtgct tgatgttgtt gaagaaacgt
      661 tcgacgaggt tgcgctcgca atcgagcgcg aagtcgcact cgcgcggcgt cttgccccct
      721 tttttcgagc cccggcgctg tgctggtggg ctctgacgac ggttgcatcg atcatggcgt
      781 actcgttgtc cggatcgtcc gctaaggtct tgaatagact ctcccaaacg ccgctttcgg
      841 cccatcggga taatcgttta tgagtgttct tccaatcgcc aaaacgttcg ggaagatcgc
      901 gccagggcgg aacttccaga tgaccgcctc cacgaacagc cggttgccct tgtcgctgtt
      961 gcgcccgaca tggccggggc ggcctggaag caaatgttca attcgtgcaa attgatcgtc
     1021 gctcaatgca tggcgtctca aaggcggcct ccatcaagaa tgctcacatg aatcgcgctt
     1081 cgcgcggtta ccgcacgcac gtcgcgccac gggcggccgt caggccgccg ctactcttag
     1141 aacaacggct cgatgatcgt ccactgctgg tcggtcaggt ccatgcccgt ccggtacgac
     1201 aggtcgaccg taaccgttta tgagaaaact tccaatctaa ccttgatcgc ggtacgctta
     1261 cgcaatgcga ccccttcggt attccatcaa cgtcacattg gacgggtgct gcgatcatcg
     1321 tgaaatgttc ccagacgaag acttgcatcg tcacgcagcc gagaacatcg cgcaggccga
     1381 tgccctcctc tttggccggg taacctacga aatgatggag gcagcgtggc ggccgccggc
     1441 gcggacggga gttaggcctg attggatggc cgattggatg gaacccttcg cccggacgat
     1501 cgacgcggca aagaagtacg tcgtgtagag caccctggac cgggtcgatt ggaacgcgga
     1561 actcgtgcgc ggggatcatc tggggaatgc cgttcagcag ctgaagcggg agtcgggtaa
     1621 gggactgttc gtgggaggcg tgaagctccc gctggcgttg gcggagctgg gattgatcga
     1681 tgagtacgag ttcgtggtgc agcccaggct ggcgggccac gggccgacgt tgttcgcggg
     1741 gctatcgaag catatcgact tgaagctcgt gagccggctg gagttcggct cgggggcggt
     1801 ggcgatgcgg tatgagccta gaaggtagcc atcgggcttg ttagcccgag tcggccgcat
     1861 catggagcaa ctcgagccag gcctgcgaat ccgctaaatg gcgacacgcc ctggtgtgct
     1921 ttgtgaacct gatcgtggtg gtcacaaaga acgtgacagc ttgcgttgag ccaaatgacc
     1981 tggcatttac agggccaaca ggtccgcgtg tctggcaccg gagcgcaagt cctcgggcac
     2041 ggtcaccagc gtgtccaggt acataaggaa cctcttcgct gtaccctgtc cgatgggatc
     2101 tacaccatga gccaggatgg aggagtttcg ggccgtgagc aggttctgga aggcggtatc
     2161 ctcctcctcc gggtcctttc cgctcaggat gtgatcgagg attctggcat gcctgagtcc
     2221 tagaccatcg ggcaactcct ttgtctgcac gagcttcagg aagtgatccc gcgcgtcttt
     2281 atcgacgcgc tcccacttaa cctttttcgt ctcgatagag tagaagctca tcagcctcca
     2341 ctggtgccac atctccaccg cccggtagag acgcgccacg ccgtcgtcgt aacggccctg
     2401 gtcggttatg cggcggcggg cgttctcgac catatccacc acgttctcta cggagagctt
     2461 gccgcgcacc ttgccgagga aaggcaggtg ggccgtaatg cgacctgtga tctctccggc
     2521 ccgttcggcg agcgccgctt ccgaaaaccc tacgcccaac tcttcccgcg cgagcttgag
     2581 cttctccagg gctgtcccgt agtcagcgac gtcccaggct ccgtagcctt cggcgaggag
     2641 caggagcccg ttgtaatagt ggcctcgctc cactcccgtg accttcctgg ctacatcctc
     2701 gaagacgaga gctgcggcgc cgtagtcgcg ccggttgaag agctcgaccg cctgaccttc
     2761 gcgcaacagt ccggtggatt caagcgggtt gtccataggg acgatctcac gttgcctgga
     2821 ggcatccagt atccattcgc cttctacgaa gcgttggggc actctctgat ggaccattcg
     2881 catgccacga gtcatcgcgg cgagggcggc gccgaagcgc atcggggtgg tgccgccggt
     2941 gacgtccagg accacgtctc ccgtggcgta gccgaggcct tcgatctccg agagcaggtg
     3001 ctcgaagcgt tcgaaggagt ctcctatctc catcggagag tccacgatac ggtaccggaa
     3061 cgttgcccta tcctccatct cctggcactt catcacgatc ggcccgagta tgtcctgcga
     3121 caggataacg cccactatct cgggccccag ttccgcgacg gcgacctcga cagcgtcggt
     3181 cctggagacg accatgataa gtgccttcgc ggacagatca ctcatttctc cggcacctcc
     3241 ggtgggcatc cctctataac gacccccatt accgggcagt aggagcaggc gtggtgcgag
     3301 ggtgtcggct cgtaggctcc ggaccgtatc gtttccagcg ctttgattac ccgctcctcg
     3361 gctctctcga ggaagccctc aagcatctcg acctcgagtc tctcgtcgcc cgggacgaag
     3421 acgtaggcga ggcgcatcct ctcgggctct acgccccaga ggcggttggc ggcgagcgcg
     3481 tagagtggga gctgtagacg gctcttgtag ctgtcgtggg gacggcctgt cttccagtcg
     3541 atcaggtgca ccgtgccgtc ctcgtcgagg aagacggcat cgatcctccc tcgcacctcg
     3601 gcgcctccga tctccacagc gaaggagacc tcgaccattc gtgcaggtcc tgtctccggg
     3661 aggtctgtgc cttcgtgcag cgggtaggaa gacgccttga gcgcgtactc cgaggtgctg
     3721 aagtcgcctg caggcgcccg gccgataccg ttcccacctg ctgcttctgg tttcggtgga
     3781 tcgccttgca tgccgccctc gatccacgcg tgcaccgcgc tgccgtactc ctgggactcc
     3841 tccatcctct ccgagcctaa tgggacgggg aagacgtagg tgtagtaata gcgcctcgcg
     3901 caggtctcca ggtccatgag ggagctcggc gagtgggtct ccgggggcgg cagtatgtgc
     3961 ctggggcgcg gcctcgtggg gatgttctcc acgtcgcgtc gcaaggcgaa gtactcatct
     4021 tcccattcct ccacgccgag ttgcgctgcg atctgtgcgt catcgccatc ggctgtcgcc
     4081 tcgatgggcc aggggtccgg cgggcctttc tccagctcgc ccgtctcctg gacgttcggg
     4141 ttgctctcag ggacctccgg ttccggcgcg aaggagatgt cgcagtcctg gggagcatcg
     4201 aggatcaggt cccagaaagc gccggg
//
LOCUS       DATAIR010000792         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_77361, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000792 DATAIR010000000
VERSION     DATAIR010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77361"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VHM69_18840"
     CDS             <1..461
                     /locus_tag="VHM69_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2742503.1"
                     /translation="KLETGTQNHEGIAGVKGALDFISSLGEGESPRERFLEAMRTVEE
                     HETRLADQFRAALREVPGVKLYAAPDGVRKTPTVAFRLDGHAPLDVCRRAADHGYFIA
                     AGDFYASTVAQRLGISQSGGFIRAGLAPYNTEEEVEGFVRVVRDLAGESS"
     gene            645..848
                     /locus_tag="VHM69_18845"
     CDS             645..848
                     /locus_tag="VHM69_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2742504.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHHTGISGEGFKTLDE
                     GAKVTYEATQGRKGMQAENVSPA"
     gene            972..>1723
                     /locus_tag="VHM69_18850"
     CDS             972..>1723
                     /locus_tag="VHM69_18850"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX2742505.1"
                     /translation="MEYLVAVLVGLALSQLATLITTVYLHRVLSHRSIRLHPALTMVM
                     RFGTWMLTSISPREWVAVHRKHHNFSDVEGDPHSPHIEGYWQIMIGNVYYYRREANDG
                     DTLEKYAADLPFDRLDKALLRRGTLGFSLTGLVLVVLFGVFGPGWGIGPGLVALATLA
                     VVYLLLNAAINGAGHTFGYKSFENDATNLRLLALITFGEGLHNNHHARPASAKLSAFS
                     GEFDPAWPVIRLLEKLHLAEVLTKADEGWENHR"
BASE COUNT          331 a          536 c          553 g          303 t
ORIGIN      
        1 aaaagctgga gaccggcacc cagaaccacg agggcatcgc cggcgtcaag ggagccctcg
       61 acttcatatc ttcccttggc gagggggaat cccctcggga gaggttcctg gaggcgatgc
      121 ggacggtaga ggagcacgag acgcggctgg ccgaccagtt ccgcgcggcg ctgcgggagg
      181 tcccgggcgt caagctctac gccgcccctg acggcgttcg caagaccccg acggtggcgt
      241 tccgtctaga tgggcacgcg ccgctcgatg tctgccggcg cgcggcggac cacggctact
      301 tcatagccgc cggggacttc tacgcgtcca cggtcgccca gaggcttgga ataagccaga
      361 gcggcggctt catccgggcg ggcctcgccc cgtacaacac cgaagaggag gtagaagggt
      421 tcgtgcgggt ggtccgggat ctcgccgggg agtcgtctta gtaggggtgt cctgatccgg
      481 gttacggtga gacttgacga caacccggtc gagtctgcta taatgctggg cgtaacgtaa
      541 gagcaaggtg ggtttacatt cgacgaagcg ccacccccga gaagatctga gggtggtttt
      601 ttgtttggtg ggggcccata aacacgaaag gtgggcgcct gaatatggcc caaggaacag
      661 tgaagtggtt cagcgacgag aagggctacg gctttatctc cccggacgac gggggtgagg
      721 acctcttcgt gcatcatacc ggtatttccg gtgagggctt caagaccctc gacgaaggcg
      781 ccaaggtcac ctacgaggcg acccagggac gcaaggggat gcaggcggag aacgtctctc
      841 ccgcttaacg tccgataact tatcggagag cgcagggccg gggttaaccc cccggccctc
      901 gtcgtaccgc aagaaaacca cgagaccgcg ccagatagag gcttcggtac tcgttctgga
      961 ggaggatttt cgtggaatac ctggtcgccg tgctcgtggg gctggcgttg agtcagttgg
     1021 caacgcttat aacaacggtc taccttcacc gcgtactgtc gcacaggtcc atccggctcc
     1081 acccggcgct tacgatggtg atgcgtttcg ggacctggat gctcaccagc atctccccgc
     1141 gcgagtgggt agccgtccac cgcaagcacc acaacttcag cgacgtcgag ggcgacccgc
     1201 acagccccca catcgagggc tactggcaga taatgatcgg caacgtctac tactaccgcc
     1261 gcgaggcgaa cgacggcgat actttggaga agtacgccgc cgacctaccg tttgatcgcc
     1321 tggacaaagc gctgctgcgc agaggcaccc tgggattttc ccttaccggc ctggtgctcg
     1381 tcgtactctt cggcgtcttc ggtcccgggt ggggtatcgg cccaggcctc gtcgccctcg
     1441 cgacgctcgc cgtcgtctac ctgctcttga acgccgccat caacggcgcg ggacacacct
     1501 tcggctacaa gagcttcgag aacgacgcga cgaatctcag gctcctggca ctcatcacct
     1561 tcggggaagg cctgcacaac aaccaccacg cacgcccggc ctcggcgaag ctctccgcct
     1621 ttagcggcga gttcgacccg gcgtggcctg tgatccggct cctggagaag ctccacctcg
     1681 ccgaggtgct cacgaaggcc gacgaaggtt gggagaacca ccg
//
LOCUS       DATAIR010000793         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_365284, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000793 DATAIR010000000
VERSION     DATAIR010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365284"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="hypE"
                     /locus_tag="VHM69_18855"
     CDS             complement(<1..923)
                     /gene="hypE"
                     /locus_tag="VHM69_18855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX2742506.1"
                     /translation="MSDREQRVLDTLEQNRRRPPKFTAPRIDMSHGAGGKATHKLIEG
                     LLVPALENPALAPLGDAALIPFGDARIALTTDSFVVRPLVFPGGSIGELAVNGTVNDL
                     AVSGATPLGLSAALIIEEGLETEVLSHEVEAMARSAERAEVSIATGDTKVVERGKGDG
                     LYIITTGVGLADPELNLSSASVRPGDKILCSGTLGDHGAAILIARGDLELEAEVLSDT
                     RPLTPLTRALKESSGPNLRFMRDPTRGGVGTVLNELARDSGYGVALWEEKLPIRDVVH
                     GACEILGIDPLYVANEGKLLAVVAPDVADTAL"
     gene            complement(920..2077)
                     /gene="hypD"
                     /locus_tag="VHM69_18860"
     CDS             complement(920..2077)
                     /gene="hypD"
                     /locus_tag="VHM69_18860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX2742507.1"
                     /translation="MKPERPKGATIKYVDEFRNAEIARAVSAEIAAITDPQRHYKLME
                     VCGGHTHTIYRHGIKDLLPDQIELVHGPGCPVCVLPMGRVDDGIAVAERPEVIFTCFG
                     DMMRVPGSKGNLLEIKAEGADIRMVYSPLDALRIARENPDREVVFFAIGFETTAPSTA
                     LTLQRAKKEGLENFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEF
                     MPEDYGKPCVVAGFEPLDLLQSVLMLMRQLDEGRCEVENQYSRIVPWEGNLAALKSMA
                     EVFELRPYFEWRGMGFIAQSALKLSDAYADFDAELRYSVPGVRVADPKACQCGEVLKG
                     VLKPWECKVFGTACTPEHPLGTLMVSSEGACAAYYNFGRKSFEKVQIGSKK"
     gene            complement(2088..2324)
                     /locus_tag="VHM69_18865"
     CDS             complement(2088..2324)
                     /locus_tag="VHM69_18865"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX2742508.1"
                     /translation="MEHTLPDSYCTLDHDGCVVCSDAGIPLRVVSIEGDDALCEDASG
                     NRTEIALELVQPVAVGEVLLTHGGVAIGKVETEA"
BASE COUNT          423 a          741 c          835 g          499 t
ORIGIN      
        1 agggcggtgt ctgcgacatc gggagctacc acggcgagga gcttgccttc gttggcgacg
       61 tagagggggt ctatgccaag gatctcacac gccccgtgca cgacgtcccg gatcgggagc
      121 ttctcctccc acagggcgac gccgtagcca gaatcgcgcg cgagctcgtt cagaacggtc
      181 ccaacaccgc cgcgagtggg gtcgcgcatg aagcgcaggt taggtcctga ggattctttg
      241 agcgcccggg tgagcggtgt caggggccgc gtatcggaaa ggacctcggc ttccagttca
      301 agatctccgc gcgcgatcag gatggctgcc ccatggtcgc ccagggtgcc tgaacacagg
      361 atcttgtccc cgggacgaac tgaagcggag gagagattca attcaggatc agccagccca
      421 acaccggtag ttatgatgta gagtccgtct cccttcccgc gctcgaccac tttggtgtcg
      481 cctgtggcga tggagacctc ggcccgttcg gctgaccgag ccatcgcttc gacctcatgg
      541 ctcaacacct cggtttcgag tccctcctcg atgatcaagg cggcggagag gccgagcggg
      601 gtggcgccgg agactgcgag gtcgttgaca gtgccgttga cggcgagctc tccgatggag
      661 ccgccgggaa agaccagtgg tcgtacgacg aaggagtcgg tggtgagggc gatgcgggcg
      721 tctccgaacg ggatcagggc ggcgtcgccg agcggggcga gcgccgggtt ttcgagggct
      781 ggcacgagaa gaccctcgat gagcttgtgg gtagccttgc ctcccgctcc gtggctcatg
      841 tctatgcgcg gggccgtgaa ctttggcggc ctgcggcggt tctgttccag cgtgtcgagg
      901 acgcgttgtt ctctatcgct cattttttgg acccgatctg gaccttctca aatgacttgc
      961 gcccgaagtt gtagtaggcg gcgcacgctc cctctgatga gaccatgagc gtaccgagcg
     1021 gatgctcggg cgtgcaggcc gtaccgaaga ccttgcattc ccacggcttg aggacgcctt
     1081 tgaggacctc gccgcactgg cacgccttcg ggtcggcgac gcgcacgcca ggaacggagt
     1141 agcgcagttc ggcgtcgaag tccgcgtatg cgtcggagag cttgagggcg gactgggcta
     1201 taaagcccat cccccgccac tcgaagtatg ggcggagctc gaagacctcg gccatgctct
     1261 tgagggctgc caggttgcct tcccagggaa cgatcctgct gtactggttc tcgacctcgc
     1321 agcgcccctc atcgagctgg cgcatgagca tcaggacgga ctgtagaagg tcgagcggct
     1381 cgaaccctgc taccacgcac ggcttgccgt agtcctccgg catgaactcg tagggcctgc
     1441 aacctatgac cgtcgagacg tgccctggcc caataaaagc gtcgaggcgc aggtccgggg
     1501 agtcgagcag cgcccgcaca ggcggaacta tggttacgtg gttgcagaat acggagaagt
     1561 tctccaggcc ctccttcttt gcccgctgta gtgtcagggc cgtcgatggg gccgtggtct
     1621 cgaagccgat ggcgaagaag acgacctcgc ggtcggggtt ctcgcgcgct atgcggaggg
     1681 catcgagagg agaatagacc atgcggatgt cggcgccctc ggcctttatt tcgagcaggt
     1741 tccctttcga gcctgggacg cgcatcatgt cgccgaagca ggtgaagatg acctcggggc
     1801 gctcggcgac ggctataccg tcgtccacgc ggcccatcgg caggacgcag acggggcaac
     1861 cagggccgtg gacgagctct atctggtcag gtaagaggtc ctttatcccg tggcggtaga
     1921 tggtgtgggt gtggcctcca cagacctcca tcagcttgta gtgtctctgg gggtcggtga
     1981 tcgccgctat ctcagcagat acggcgcgtg cgatctcggc gtttctgaac tcgtctacgt
     2041 atttgatggt cgctcccttc ggtcgctcag gtttcaggca tcaggtatca ggcttcagtc
     2101 tcgactttgc cgatggcgac gcctccgtgc gtgagcagca cttctcccac ggctaccggc
     2161 tggaccagtt ccaatgcgat ctcggtgcgg tttccggagg cgtcttcgca gagggcgtcg
     2221 tcgccttcga tggagacgac cctaaggggg atgcctgcgt cggagcagac gacgcagccg
     2281 tcgtggtcga gggtgcagta ggaatcaggg agtgtatgtt ccacggctac tctatctggg
     2341 aggcgcgtag ctctgccagc tcctgctcgt aggcctcacc gatctcttcg agggcctgta
     2401 gctgggactg tgcctcggtt tcgttcagcc gcgacatcgc gaatccgacg tggatcagga
     2461 cccagtctcc gacttcgacc tgctcgtcct cgttgtgg
//
LOCUS       DATAIR010000794         6925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_365354, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000794 DATAIR010000000
VERSION     DATAIR010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365354"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VHM69_18870"
     CDS             <1..954
                     /locus_tag="VHM69_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HEX2742509.1"
                     /translation="VYIEEFLQVFEAMKSIFGFEQISMGVHEKDREWFADYAEHADDE
                     VFDIRYVPNTYALGEEKTLVKHATEARVPRFLDTPDGMRRPGMPPDVGKVVNNTETLL
                     NVYNAFFLGKPLTTKFFTVYGEDVDIKVYETPIGASVSEVLGIAGLDVENSGHLTVLD
                     GGPYLHDVSIKEIGTGDAYVRRMTNSLFLIPRGRQGKEYADIETDCPEEGIVSLVSKV
                     SGVNLPLGGGLLKPATPLVSEGDEVEYEQKIGEPVDEGFSVGVWASVGGEISSIENGI
                     VAISGGAIPQEEAKAEAEPSMAGGESPRGEAEPFQEADSSR"
     gene            complement(1045..1872)
                     /locus_tag="VHM69_18875"
     CDS             complement(1045..1872)
                     /locus_tag="VHM69_18875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742510.1"
                     /translation="MPWNLRSRVTLSGGEIAYDVLGYGPPLVLVHGTPGRSYIWREVA
                     MTLANRFTVYSYDLLGFGQSERREGLDVSIAAQSRLLAELLEVWGLDAPYIAGHDIGG
                     GILLRAHLLEGMPFRSIALVDSVVLKPWITPTTRHVKAHLDVYETMPTHIFEAIIASH
                     LRTATHRPMDDDAFPTYLDQWKGEFGQKLYLQKDAQLDEDDTAQFESLLPVVEVPVRI
                     VWGENDAWLAPSQAERLHDIIPTSDLVLLPETGHFAMEDSPREVAAALFQFFTVLGR"
     gene            complement(1872..3005)
                     /locus_tag="VHM69_18880"
     CDS             complement(1872..3005)
                     /locus_tag="VHM69_18880"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2742511.1"
                     /translation="MENLLAVIAENTEVLALTRQLVRYRSYTPGASQSAMDFINGWFG
                     ARGLETISYTGAGPRGDLPSLVVRVGTGDPTILLHGHVDVVPGDESQFEPREEEDELY
                     GRGVYDMKGALAAMMYAVEDLHAIGSEATVELLVVPDEEGEHGAKTGAEVLIGNGHVG
                     DFVITGEPTDFHIGTQAKGVLDVRVTLRGKSAHGSQPWLGKNAALMAYEHYHKVVDLP
                     FAKERSELFPYPSVNLARIIGGDVLNRVPDRCTYDLDVRYLPNQDPTEIVRQLRSVDL
                     PADVEVLFYREPTYVSRKNPYVKVLREVAARNFRGNPVGVGRHGASDVVYFERAGVPG
                     VEFGPVGGGHHGPSEYVVTSSLETYRRMLVQFVQIVGRNGKVV"
     gene            complement(3140..3928)
                     /locus_tag="VHM69_18885"
     CDS             complement(3140..3928)
                     /locus_tag="VHM69_18885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2742512.1"
                     /translation="MDGGEFVLLASPAWRTRVEREWAHDVARHAQQLVSQVVAPVRRS
                     GRTVFECEGRFVAVYPFVVGQMMDREDRVLRYEAAAILAAIHNALIDWRGGPRPPSGE
                     SRPAPPPDPPDLHDPALDAWWLSVQDRTFLVSATHGDYYRRNLLCAKRKIVGLIDWHD
                     ATVRPLAVELAGATFEMCRDDEHMLQLDRTDEFVTSYLAAGGPLPEHELDVLLPLIRL
                     WIRNDARGSLTHKEQTDYEYAARQMRAFRTLASCEWRPSSVTGG"
     gene            complement(4134..5423)
                     /gene="murA"
                     /locus_tag="VHM69_18890"
     CDS             complement(4134..5423)
                     /gene="murA"
                     /locus_tag="VHM69_18890"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX2742513.1"
                     /translation="MSERFYVEGGKRLQGKVEVSGAKNAALKFVAAALLAPGKTVLHD
                     VPRIKDMHTMLALLDELGVRTTFSGSTLEIDASTVDSYTAPYELVRQMRASLVVLGPL
                     AARFGEAEVSAPGGCNLGLRKFNFHVDGLRALGADIELDHGFIKASAPNGLRGTEVTF
                     EYPSVGATENVMMAAVLADGITIIENGAREPEIVALQGFLNSMGARVMGAGTGRIVIE
                     GVEELRPTEWTVMPDRIEAGTFIMAAAATGGDVTVVNAVPEHLKMELEKLREMGVDVD
                     ATPGSPGIRARVEDPALLTGVDVATLPFPGFATDLQAQMMVLLTQASGAGIITENVYE
                     NRLQVAEELNRLNAGIDLFGGHRALVRGPRRLSSAIVQSPDLRGGAALVMAGLVADGT
                     TVVDGAKHILRGYENFEEKLSGLGATVAFEAGAPVSR"
     gene            complement(5576..6031)
                     /gene="atpC"
                     /locus_tag="VHM69_18895"
     CDS             complement(5576..6031)
                     /gene="atpC"
                     /locus_tag="VHM69_18895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX2742514.1"
                     /translation="MAENREERGGGRQLFCRVITPEEVIYDGEANLVIARIADGDLGV
                     LVDHSPLVSTVEIGEVRIRENDEQLVYATSDGFFKVSENLVQVLVEEAVSVEEIDVDE
                     AENRVEKAEKELSEVPEDAEDRDRMVAEITRRQRMGENLVRLARKYAET"
     gene            complement(6031..>6925)
                     /gene="atpD"
                     /locus_tag="VHM69_18900"
     CDS             complement(6031..>6925)
                     /gene="atpD"
                     /locus_tag="VHM69_18900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2742515.1"
                     /translation="MNEPPGARFRVALTGVTIAEYFREELGQDVLFFVDNIFRFVQSG
                     NEVSALMGRMPSEVGYQPTLGTEMGALQERITSTKKGSITSFQAVYVPADDFTDPAPF
                     TVFAHLDSTVELSRSLSAQGIYPAVDPLTSSSTMLDPSIVGDEHYEVAQQVQNILQRY
                     KELQDIIAILGIDELSEEDKVIVGRARRLQRFLSQPFFVAEQFTGLEGKFVELDETIR
                     SFKEVVEGKHDDLPEQAFYLVGTIDEAVEKARKLGGEEDESGDEESEEEESGQAEASS
                     EDSDEGSRDEESGEAGEDNEK"
BASE COUNT         1265 a         2243 c         2124 g         1293 t
ORIGIN      
        1 gtgtacatcg aggagttcct ccaggttttc gaggccatga agtccatctt cgggttcgag
       61 cagatctcca tgggcgttca cgagaaggac cgggagtggt tcgccgacta cgccgagcac
      121 gcagacgacg aagtgttcga tatacgatac gtgcccaaca cgtacgccct gggcgaggag
      181 aagaccctcg tcaagcacgc tacggaagcg agagtacccc ggttcctcga cacccccgac
      241 ggcatgaggc gtcccgggat gcccccggac gtcggcaagg tcgtcaacaa cacggagacc
      301 ctgctcaacg tctacaacgc cttcttcctg ggcaagcccc tcaccacgaa attcttcacg
      361 gtctacggcg aggacgtgga cataaaagtc tacgaaacgc ccataggggc ctcggtgagc
      421 gaggtacttg ggatcgctgg cctggacgtc gagaactccg gccacctgac ggttttggac
      481 ggtgggccct acctgcacga tgtgagcatc aaggagatcg ggacgggcga cgcctacgtg
      541 aggcggatga ccaactccct cttcttgatc ccccgcggcc ggcaaggcaa ggagtatgcc
      601 gatatagaga cggattgccc cgaagagggc atcgtctccc tggtcagcaa ggtctccggc
      661 gtgaacctgc ccctgggcgg cggtttgctg aagcctgcca cgccactagt ctccgaaggc
      721 gacgaggtcg agtacgagca gaagatagga gagcccgtcg atgagggctt ttccgtaggc
      781 gtctgggcta gcgtgggggg tgagatctcc tccatcgaga acggcatcgt cgccatctcc
      841 ggcggcgcga taccccagga agaagccaaa gccgaagcag aaccgtccat ggcgggcgga
      901 gagtcacccc gtggggaggc agagcctttc caggaggctg actcgagcag gtaaaagaag
      961 gtgcgttagg gcgttgcgta ccggaatgtg ctctgatctc ctatttccga ctaccgaaca
     1021 gaccggtacg gtggcactga cacttcatct ccccaggacg gtgaagaatt ggaagagcgc
     1081 cgctgcgacc tctcgggggc tgtcctccat cgcgaaatga cctgtctcgg gcagaagcac
     1141 gaggtcggag gtggggatga tgtcgtgcag gcgttccgcc tggctcggag ctagccaggc
     1201 gtcgttttcg ccccagacga tcctgacggg aacctcgacc actggtagaa gagactcgaa
     1261 ctgcgcggta tcgtcttcat cgagctgggc gtctttctgg aggtagagct tctgcccgaa
     1321 ctcacccttc cattggtcga ggtaggtcgg gaacgcgtca tcatccatcg gcctgtgcgt
     1381 cgccgtacgc aggtgggagg cgatgatcgc ctcgaagata tgggtcggca tggtttcgta
     1441 cacgtcgagg tgtgctttca cgtgcctggt cgtcggggtt atccacggtt tgaggacgac
     1501 gctgtcgaca agggcgatac tcctgaaggg catcccttcg agcagatggg cgcgcagtaa
     1561 gatcccgccc ccgatatcgt ggccggcgat gtatggagca tcgagccccc agacttccag
     1621 cagctcggcg agcaggcgtg attgcgccgc gattgagacg tccagtccct ccctccgctc
     1681 cgactgcccg aaccccagga ggtcgtatga gtagacggtg aatctattcg cgagggtcat
     1741 ggcgacttcg cgccagatgt atgagcggcc cggcgtcccg tgtaccagga ccagcggtgg
     1801 tccgtagccg agcacgtcgt aagcgatctc acctcccgag agcgtgacgc gactacgcag
     1861 gttccagggc actagactac cttgccgttg cggccgacga tctggacgaa ctggacgagc
     1921 atccgacggt aggtttccag ggaggaggtc acgacgtact cgcttgggcc gtggtgtcca
     1981 ccgccgacgg gaccgaactc gacgcctggc acgcccgccc gctcgaagta gacgacgtcg
     2041 cttgcgccgt ggcggccgac tcccacgggg ttgccgcgga agttgcgcgc tgccacctcg
     2101 cgcaggactt tgacgtaggg gtttttgcgc gatacgtagg tgggttcacg gtagaacagc
     2161 acctcgacat ccgcgggcag atccaccgag cgcaactgac gcacgatctc cgtcggatcc
     2221 tggttcggga ggtagcggac gtccaggtcg tacgtacatc ggtcggggac gcggttgagg
     2281 acgtccccgc cgatgatgcg cgccaggttc acgctcggat acgggaacag ttcgctgcgc
     2341 tctttggcga aaggcaggtc cacgacctta tggtagtgct cgtaggccat gagcgcggcg
     2401 ttcttgccca gccagggctg ggagccgtgg gcgctcttgc cgcgcagcgt cacgcgaacg
     2461 tcgagcacgc ccttcgcctg cgtacctata tggaaatcgg tcggctcgcc ggtgatcacg
     2521 aagtccccga catggccgtt cccgatcagg acctccgcgc cggtcttggc gccgtgctca
     2581 ccctcttcgt cggggaccac gagaagctcg acggtggcct cggagccgat ggcatgcagg
     2641 tcctcgacag cgtacatcat cgccgcgagg gcacccttca tgtcgtatac gccgcggccg
     2701 tagagctcgt cttcctcctc gcgcggctcg aactgcgact cgtcgcctgg cacgacgtcg
     2761 acgtggccgt gcaggagaat cgtgggatca cccgtcccga cccgcaccac gagcgagggc
     2821 aggtcaccgc gcggcccggc gcccgtgtaa ctaatcgtct cgagtccccg agccccgaac
     2881 cagccgttga tgaaatccat cgccgactgc gaggcgcccg gcgtataaga cctgtagcgc
     2941 acgagctgcc gggtgagcgc aaggacctcg gtattctcag caattaccgc cagaaggttc
     3001 tccacttttc agccgccatc cagagcagtc tattctaaaa tatggaaggc gtctctaagt
     3061 attggagatc agcccggaga gttagtagcg accgatcaca aagaagatga tcctcgcgaa
     3121 ccgactcccc cagttacgtt catcctccgg tcacgctgct cggcctccat tcgcaggacg
     3181 cgagcgtgcg gaacgcccgc atttgcctgg cagcgtattc atagtcggtc tgttctttgt
     3241 gtgtgagtga ccctctggca tcattgcgga tccacaagcg gatcaggggt agcaaaacgt
     3301 ccagctcatg ctcgggcaat ggaccaccag cggccaggta cgaagtgacg aactcgtccg
     3361 tgcgatccag ttgtagcatg tgctcatcgt cccggcacat ttcgaacgtc gcgcctgcga
     3421 gttcaactgc gagcgggcgg acggttgcgt cgtgccagtc gatcaatcca acgatcttcc
     3481 ttttcgcgca gagcaggttg cgccgatagt aatctccatg cgttgccgac accaggaacg
     3541 ttcgatcctg taccgacagc caccacgcgt cgagtgcagg atcgtgcaga tccggcggat
     3601 cgggcggcgg cgcgggtcta ctctcaccgg acggcgggcg cgggccgccg cgccaatcta
     3661 tgagggcgtt atggatcgct gcgaggatcg ccgcagcttc gtagcgaagg acacggtcct
     3721 ctcggtccat catctggccg acaacgaacg ggtacactgc cacaaaccgc ccctcgcact
     3781 cgaaaactgt ccgaccgctt cgccggaccg gggcgacgac ctgcgagacg agctgctgcg
     3841 cgtgcctggc aacatcgtgt gcccactcac gttccacgcg cgtccgccag gctggactcg
     3901 caagcaggac gaactcgccc ccgtccatct cgacgagcag gacagtcgct tcatctccgc
     3961 ccgcgagccg cgcagatgac taaaccgcaa cgccgaggaa ccgctcggca gcgcgcacca
     4021 ggtagggttc aaggatcaaa tccggctgtg gcatcgaacc cccaccgcca ttccaccgat
     4081 cagttaccca tattcgctcc atagctcgca gacatcgcag cgagctagag catctagcga
     4141 ctaaccgggg cccctgcctc gaaggcgacg gtcgccccga gccccgagag cttctcctcg
     4201 aagttctcgt agcctctcag gatgtgcttc gccccgtcca ctaccgtcgt gccatcggcg
     4261 accagtccag ccattacaag cgcggcaccc cctcgcaggt cgggcgactg cacgatggcg
     4321 ctcgagagcc tgcgtgggcc gcggacgagc gccctgtggc cgccgaagag gtcgatgccg
     4381 gcgttgaggc ggttgagctc ctcggccacc tggagcctgt tctcgtagac gttctcagtg
     4441 atgatgcccg cccccgaagc ctgggtcagc agcaccatca tctgtgcctg gaggtccgtc
     4501 gcgaagccag ggaatggaag ggtggcgaca tcgacgcccg tgagtaaggc gggatcctcg
     4561 acccgcgccc tgatgcctgg ggaaccggga gtcgcgtcca cgtccacgcc catctcccgc
     4621 agcttctcga gctccatctt caggtgctct ggaacggcgt tcacgaccgt tacgtcgccg
     4681 cctgtcgccg cggcggccat gataaacgtg ccggcctcga tgcggtcggg catcaccgtc
     4741 cactccgtgg gccgaagctc ctctacgccc tcgatcacga tgcggcccgt gcccgctccc
     4801 atgacgcgcg cgcccatcga gttgaggaag ccctgcaggg cgacgatctc gggctcgcgg
     4861 gcgccgttct cgatgatcgt gatcccatcc gccagtacgg cggccatcat gacgttctcg
     4921 gtcgcgccta cgctggggta ctcgaaggtg acctctgtgc cgcgcaaccc gttcggagcg
     4981 ctcgccttta tgaagccgtg gtcgagctcg atgtctgcgc cgagcgccct gaggccgtcg
     5041 acgtggaagt tgaacttcct gagacccagg ttgcacccgc cgggcgcgga tacctcggcc
     5101 tcgccgaagc gcgccgcgag cgggccgagg acgacgaggc ttgcgcgcat ctggcgcacc
     5161 agctcgtaag gggccgtata cgagtccact gtggaggcgt cgatctccag ggtgctcccc
     5221 gagaaggtcg tccgcacacc gagctcgtcc agcagggcga gcatcgtgtg catgtccttg
     5281 atgcggggaa cgtcgtgaag gaccgtcttc cccggcgcca ggagcgcggc cgcgacgaac
     5341 ttcagggcag cgttcttcgc ccccgagacc tcgaccttgc cctgaaggcg tttaccgccc
     5401 tcgacgtaga atctctcaga cacggatcct ccccggtatc tgcttcccgg cccgctcttc
     5461 cggactcgta gtatcactat gcccacgaat agttacgaaa cggcaacaac cggataacgt
     5521 ctccgttacg accggcgggc cgtttcgaca tgcgtggatt ctagcctttt cggctctacg
     5581 tttctgcgta cttgcgcgcc aatcgcacca ggttctcgcc catgcgctgg cggcgcgtta
     5641 tctcggcgac catcctatca cgatcctcgg catcttccgg aacctctgag agctcctttt
     5701 ccgccttctc gacgcggttc tcagcctcgt caacgtcgat ctcctctacg gagacagcct
     5761 cctcgacgag cacctggacc aggttctcgg agaccttgaa gaagccgtca gaggtcgcat
     5821 agacgagttg ttcgtcgttc tccctgatgc gtacctcccc tatctccacg gtggagacca
     5881 gcggagaatg gtcgacgagc accccgagat ctccgtccgc tatgcgggcg atcaccaggt
     5941 tggcttcacc gtcgtagatg acctcctcag gcgtgatgac ccggcaaaat agctgcctcc
     6001 caccaccccg ctcctcgcga ttctcagcca ctacttctcg ttatcctcgc cggcttcgcc
     6061 tgactcctcg tcccgagagc cttcatcgga gtcttcgcta ctggcttcag cctgaccgga
     6121 ctcttcttcc tcggactcct cgtctcctga ctcgtcctct tcaccgccga gcttgcgggc
     6181 cttctcgacg gcttcgtcga ttgtgccaac gagatagaag gcctgctcgg gcaggtcgtc
     6241 gtgcttgccc tcgacgacct ccttgaagga ccggatcgtc tcgtcaagct cgacgaactt
     6301 gccttcgagg cccgtgaact gctcggctac gaagaacggc tgtgagagga aacgctgaag
     6361 gcgccgcgcc cgcccgacga tgaccttgtc ctcctccgag agctcgtcta tgccgaggat
     6421 cgcgatgatg tcctgtagct ccttgtagcg ctgtaggatg ttctgcacct gctgcgccac
     6481 ctcatagtgt tcgtcgccaa cgatggacgg gtccagcatc gtcgaggacg acgtcagagg
     6541 atcgacggca gggtagatac cctgggccga cagggatcgc gagagctcga ccgtcgagtc
     6601 gaggtgggcg aacacggtga aaggcgcggg atcggtgaag tcgtcggcag gcacgtagac
     6661 ggcctggaag ctcgtgatcg aacccttctt cgtcgaggtg atcctctcct gcagcgcccc
     6721 catctcggtc ccgagggttg gctgatagcc gacctctgag ggcatgcggc ccatcagcgc
     6781 cgagacctcg ttgccggact ggacgaatcg gaagatgttg tctacgaaga agagcacgtc
     6841 ctggcctagc tcttcgcgga agtactcggc gatagtgaca cccgtcagcg cgacgcggaa
     6901 acgggcgccc ggcggctcgt tcatc
//
LOCUS       DATAIR010000795         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_227803, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000795 DATAIR010000000
VERSION     DATAIR010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227803"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VHM69_18905"
     CDS             <1..593
                     /locus_tag="VHM69_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2742516.1"
                     /translation="FIPGEVGNYPLPPEAWSHEVLVQAARLLRRYHDATVGYAPPGSA
                     IWQLRAPFGPREVIRHNDFAPYNLVFADRSPRAIIYFDMAGPGPRVWDVSYAAYCFVP
                     LSGKEHSLGLPAPVDTNRRLRTFCDTYGLATEQRRALPETIEIRLKTLCAFIERSAAT
                     GDSRVERVIEEGQLALYRRDIELLRRRRVELQKSLE"
     gene            964..1503
                     /locus_tag="VHM69_18910"
     CDS             964..1503
                     /locus_tag="VHM69_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742517.1"
                     /translation="MREDMQYISGVLDELEAIVQDASGVPMSKGRAVVDRSDLLVMLD
                     ELRGSLPPELAEAEALRRECGAQVAAAEEEAQRIVEEAHHRANAMVPETELYRRAQRR
                     AGEVIDGAERYAEEVSDGSEVYRDRVMGQLENWFQDSLVSVEESRQELGGIPVRRPAP
                     RPEVVEEKTDGQGWQASSA"
     gene            complement(1630..>2337)
                     /locus_tag="VHM69_18915"
     CDS             complement(1630..>2337)
                     /locus_tag="VHM69_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742518.1"
                     /translation="ALRPLNALVLVIGAGIFLTTVSATALAWLLLSLTLVTYASLVFL
                     SARDPIFQRKVLGEDEPQATSRNVVEDVSPERRARWLPRGETRQKVEAALDVYRNVVA
                     AIEDSDDVTREVLHDAVPRLHAAADRLVDVASGREKAAETVRDLHRETGSSAGTAARQ
                     ENLRKLEERIDVADAEISDTFEELLDLRAKVVRISIDSENPARANDLNASLDGLNARL
                     EALSEVMSPQGELPREP"
BASE COUNT          462 a          697 c          744 g          434 t
ORIGIN      
        1 cattcattcc gggtgaggtc ggaaactacc cgcttcctcc agaagcgtgg tctcacgagg
       61 tgctcgtgca agccgcccgc ctcctgcgcc gttaccacga tgcgaccgta ggatacgctc
      121 ccccgggcag cgcgatctgg caactccggg cccctttcgg tccccgcgaa gtgatccgcc
      181 acaacgactt cgcgccctac aacctcgtct tcgcggaccg gagtccccgc gccatcatct
      241 acttcgacat ggccggcccc ggcccgaggg tatgggacgt ctcctacgca gcctactgct
      301 ttgtcccact ctcaggcaaa gagcactcac tcggcctgcc cgcgcctgtg gacacgaacc
      361 ggcgtctccg cacgttctgc gacacttacg gcctcgcaac tgaacagagg agggcgttgc
      421 ccgagacaat cgagatcagg ctgaagacgt tatgcgcgtt catcgagaga tccgccgcaa
      481 caggagactc cagagtcgag agggtgatcg aggaagggca actagccctc tacaggcgcg
      541 acatcgagct cctgcgccgt cgccgagtcg agcttcagaa gagcctggag tagttgctcg
      601 tagagtgagt gacgatcacc cctcaagaca cccctgtccg cgcgccgaag aatgaacgga
      661 gcgtgagtca tgatgccaat aggctccgcg gcagccggaa ctcgtggaag ctggcccctt
      721 actctccttt ctaatgtagt tgaaccccgg ttcaccggcg agtcaagcct gtggataact
      781 tgctcaaccc cactaataga gctaaaaacc gggctttcca aggcacatcg gggtacttaa
      841 ccatcgtgta acagagttac acttaacctt aagatcaaat tagacctgaa ggtgaggtaa
      901 agcatcgaaa aatactttca gaaagcctag cgtagcctgt aagagcgtgc tagggttggg
      961 gccatgagag aagacatgca gtacataagc ggggttttgg acgagctgga ggcgatcgtt
     1021 caggatgcat ctggcgtacc gatgagcaaa gggagggccg tcgttgaccg gtctgacctg
     1081 cttgtgatgc tggacgagct aaggggatcg ttgccccctg agctggcgga ggccgaggcg
     1141 cttcgccggg aatgcggggc ccaagtcgcg gcggccgagg aggaggccca gcgcatagtc
     1201 gaggaagcgc accatcgtgc gaacgcgatg gtccccgaga cggagctcta ccggcgcgcg
     1261 cagcgtcggg cgggagaagt catagacggg gcggagcgct acgctgagga ggtctccgac
     1321 ggctccgaag tatatcggga tcgcgtcatg ggacagctgg agaactggtt ccaggactca
     1381 ctcgtctccg tggaggagtc caggcaggag ttgggtggca tccctgtgcg caggcccgcg
     1441 ccccggccag aggtcgttga ggagaagacc gacggccagg gttggcaggc gagttcggcc
     1501 tagccgaagc actccacagt aacgagaggt gcccgcactt tcggtgcctt gggtccttca
     1561 cgctcacgtg tccgcacgta gagacgttat attcgtatgt gatctaatga tcctctgatg
     1621 agagaagcct cagggttctc tcggaagctc tccttgcggg ctcatgactt cgctgagggc
     1681 ttcgagccgt gcattgaggc cgtcgaggga agcgttgagg tcgttcgcgc gggcggggtt
     1741 ctcgctgtct atggagatcc ggacgacctt cgccctgagg tccagaagct cttcgaaagt
     1801 gtccgagatc tcggcgtccg caacgtctat tctctcttcc agcttgcgaa ggttctcctg
     1861 cctggctgcg gttcccgcgg aagagccggt ctcgcggtgg agatcgcgca ccgtctcggc
     1921 ggctttttcc ctgccgctgg cgacgtcgac cagccgatcc gccgccgcgt gtagcctggg
     1981 tactgcatcg tggaggacct ccctggtcac gtcgtcggag tcctcgatgg cggcgacgac
     2041 gttgcggtag acgtcgagag cggcctccac cttctgacgg gtctccccgc gtgggagcca
     2101 gcgggcacgg cgctccggag agacatcttc aactacgtta cgcgacgttg cctggggttc
     2161 atcctccccg aggaccttgc gctggaagat tggatccctc gctgaaagaa agaccaggga
     2221 agcgtaggta acgagggtca gggatagaag caaccacgcc agggcggtcg ccgacacggt
     2281 cgtaaggaag attcccgccc cgatcacgag caccagggcg ttgagcgggc gcagcgc
//
LOCUS       DATAIR010000796         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_365516, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000796 DATAIR010000000
VERSION     DATAIR010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365516"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHM69_18920"
     CDS             complement(<1..281)
                     /locus_tag="VHM69_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX2742519.1"
                     /translation="MGRAPNFLEISLFSVMWSEHCGYKNSRPLLKRFPNEGDRVLQGP
                     GENAGVVEVGDGWALAFKMESHNHPSAVEPYEGAATGVGGIIRDILAMGA"
     gene            complement(347..997)
                     /gene="purQ"
                     /locus_tag="VHM69_18925"
     CDS             complement(347..997)
                     /gene="purQ"
                     /locus_tag="VHM69_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2742520.1"
                     /translation="MTVFPGSNCDRDAIYGVERMGAEAVELWHADADLKNVDAVIVPG
                     GFSYGDYLRPGAIARFAKVMSPLEEFAKEGGPVLGVCNGFQVLTEAHLLPGALLRNTH
                     MRFVCDRVRVRVESMDTPWTAGCDPGEELTLPVAHNEGNYFADAETIAGLEDEGRVVL
                     RYLENPNGSANDIAGVCNEGRNVVGIMPHPERVSDPTLGSDEGLRILRSVLVGAGV"
     gene            complement(1006..1242)
                     /gene="purS"
                     /locus_tag="VHM69_18930"
     CDS             complement(1006..1242)
                     /gene="purS"
                     /locus_tag="VHM69_18930"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2742521.1"
                     /translation="MADNLKIRVLVRPKAGILDPQGEAVKSALPALGFEGVHTVHVGR
                     LIEMEVESADEVDAMCRRLLSNPTIEEYEWEVTG"
     gene            complement(1418..>1598)
                     /locus_tag="VHM69_18935"
     CDS             complement(1418..>1598)
                     /locus_tag="VHM69_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2742522.1"
                     /translation="DGDLMLADEISPDTCRFWDKETGEKLDKDRFRNDMGGVEEAYAE
                     MLRRVTEITQRGAVD"
BASE COUNT          269 a          538 c          470 g          321 t
ORIGIN      
        1 gcgcccatcg ccaggatgtc gcggatgatc ccacctactc ccgtcgcggc cccctcatag
       61 ggttctacgg cagagggatg attgtggctc tccatcttga aggcgagcgc ccacccgtcc
      121 cccacctcga ccacgccggc gttctccccc ggcccctgca agaccctgtc cccctcgttc
      181 gggaagcgct ttagaagtgg tcgcgagttc ttgtagccgc agtgctcgct ccacatcacg
      241 gagaagagcg agatctcgag gaagtttggt gcccgcccca tgagctccag gatccggtcg
      301 tactcgaagt ccgagagtcc gagagcgctg tatgtctcct ggatattcag accccggctc
      361 ccacgagtac ggaacgcagt atccgcaagc cctcgtcgga accgagagtc gggtcagaaa
      421 ccctttcggg atgcggcatg atgccgacga cgttgcggcc ctcattgcag actccggcga
      481 tgtcgttagc cgagccgtta gggttctcca ggtagcgcaa cactacgcgt ccctcgtcct
      541 ccagcccagc gatcgtctca gcgtcggcga aatagtttcc ctcgttgtgg gcgactggca
      601 aggtcagctc ttcgccggga tcacagcctg ctgtccacgg cgtgtccatg ctctcgacac
      661 ggacacgcac acggtcgcag acgaagcgca tgtgcgtatt gcggagcaga gcgccgggga
      721 gaaggtgcgc ctcggtgagg acctggaagc cgttgcagac gccgaggaca gggccacctt
      781 ctttggcgaa ctcttccagt ggagacatga ccttcgcgaa gcgtgcgatg gcgcctggtc
      841 tcaagtagtc accgtatgag aagccgccgg ggactatcac ggcgtccacg ttcttgaggt
      901 cggcgtcggc gtgccagagc tcgaccgcct ccgcgcccat ccgctccacg ccgtagatgg
      961 cgtcgcggtc gcagttcgag cccgggaaga ccgtcacgcc aatcctcacc cggtcacctc
     1021 ccactcgtac tcctctatcg tcgggttcga aaggaggcgt cggcacatcg cgtcaacctc
     1081 gtcggcgctc tcgacctcca tctcgatcag gcgtccaacg tggacggtat ggaccccctc
     1141 gaagccgagc gcgggaagcg cgctcttcac ggcctcgccc tgcgggtcga ggatgcccgc
     1201 cttcggacgg acgaggacac ggatcttcaa gttgtcagcc atcaggtttc agctctcgct
     1261 gaaggtttca ggctacaggt ttcaggcttc aggttttctc gtccgctcgc tacggttgcc
     1321 gcagaaacga ccgggtcggg cagcggcgct ttgacgttcg atctcgcctc cgccggcgcg
     1381 atgcccgtct cattaccgct tccctgatgc ctacttttca atctactgcg ccccgctgcg
     1441 tgatctccgt gaccctgcga agcatctcgg cgtaggcctc ttcgacgccg cccatgtcat
     1501 tgcggaagcg gtccttgtcc agtttctcgc ctgtctcctt gtcccagaag cggcaggtat
     1561 cagggctgat ctcatctgca agcatgaggt cgccgtcg
//
LOCUS       DATAIR010000797         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_272067, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000797 DATAIR010000000
VERSION     DATAIR010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272067"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VHM69_18940"
     CDS             complement(<1..620)
                     /locus_tag="VHM69_18940"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX2742523.1"
                     /translation="MATRQSKPGAIKSSATDHQELEWQFDVDDLEPVEGWLGQHSSGS
                     GVVPEDTVEITDTYYDTDDWRFYRAGYALRVRKTNGGVEATMKSLSPAEGGLRRRREI
                     SEPLKDDRPATLRKARGPVGKRLKVLLGGREISPLFQVQTRRKRFALLLKSSNDGDLE
                     DVRIGEVALDSSEFGDEPERLKRVEVEAVGGTSPDLHGFVDEMQFAL"
     gene            complement(751..>2129)
                     /gene="ppk1"
                     /locus_tag="VHM69_18945"
     CDS             complement(751..>2129)
                     /gene="ppk1"
                     /locus_tag="VHM69_18945"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX2742524.1"
                     /translation="YVFQVTRNADFVIEEDEASDLLQAIEDELESRWFGQSVRIVVSD
                     EMPDDLREWLAGNLKLNENSVFAAPHPIGLADLEELTHLERSDLLYPPITPRVPSEIR
                     SARSITQAIRNGDILLYHPYDSFAPVVDFVRAAASDPEVLAIKQTLYRVGSNSPIVEK
                     LSEARDEQTQVAVLVELKARFDEEPNITWARQLEARGVHVAYGIVGLKTHAKISLVVR
                     REGHGLRRYVHLGTGNYNPGTARIYTDFSYFTDDPKLAEDGSDLFNYLTGYSEQEEYQ
                     ELLVAPLTMRENIERMIEEQTEKARNGETVYITCKTNSLTDPRIIELLYEASQAGVKI
                     DLIVRSICCLRPGVEGVSENIRVVSLVGRFLEHARIFAFSEGDDEKIYLGSADLMQRN
                     LDRRVETAFPLREKRHREKVRRLLDLQLADNANGWELGPDGTFERLHPKEGEEPVDSQ
                     AILLGEGF"
BASE COUNT          359 a          661 c          672 g          437 t
ORIGIN      
        1 agggcgaact gcatctcgtc aacgaagcca tgcaggtcag ggctcgtgcc gcccactgcc
       61 tcgacctcga cgcgcttcag ccgctctggc tcatcgccaa actccgagga gtcgagcgcg
      121 acctcgccga tgcggacatc ctccagatcc ccatcatttg agctcttgag caagagcgcg
      181 aacctcttgc ggcgggtctg gacctggaac agcggactta tctcgcgccc gccgaggagc
      241 accttcaacc gcttgccgac ggggccacgg gccttcctga gcgttgcggg tctatcgtcc
      301 tttagcggct cggagatctc tcgccgcctc cggagaccac cctcggcggg cgatagcgac
      361 ttcatcgtcg cctccacgcc gccgttagtc ttgcggacgc gcagggcgta gccagccctg
      421 tagaaacgcc agtcgtcggt gtcgtagtaa gtgtcggtga tctccacggt atcttcaggg
      481 acgacgcccg agccggagga gtgctggccg agccagccct cgacgggctc gagatcgtcg
      541 acgtcgaact gccactctaa ttcctggtga tccgtggcgg aggacttgat cgcacctggt
      601 ttgctctgtc gcgttgccat tctcgcctcg tctcgttagc ctattgcggc tatgcttacc
      661 ttaccccgag aagacagagg cgcaatctag cacgtaaccg aatcgctagc aacactgtta
      721 gcaacaaagg cgcacattag cgtcttcggg ctagaagcct tcaccgagca gtatcgcctg
      781 gctgtctact ggctcctcac cttccttcgg gtgcaggcgt tcgaaagtcc cgtcggggcc
      841 gagctcccag ccgttcgcgt tgtcggcgag ctggaggtcg agcaggcggc ggaccttctc
      901 gcggtggcgc ttctcgcgca gcgggaaggc cgtctcgacg cgcctgtcga ggttgcgctg
      961 catcaggtct gcgcttccga ggtagatctt ctcgtcgtca ccttcgctga aggcgaagat
     1021 gcgggcgtgc tcgaggaagc gaccgacgag agagacgacg cggatgttct cgctaacccc
     1081 ctcaacaccg ggcctgaggc agcagatgct gcgcacgatg aggtcaatct tcaccccggc
     1141 ctgggatgct tcgtacagga gctcgatgat ccgcgggtcc gtgagcgagt tggtcttgca
     1201 cgtgatatat acggtctccc cgttacgcgc cttctctgtc tgctcctcga tcatgcgctc
     1261 tatgttctca cgcatggtca gtggggcgac gagtagctcc tggtactcct cctgctccga
     1321 gtagccggtc aggtagttga agaggtcgga tccgtcctcg gcgagcttgg ggtcgtccgt
     1381 gaagtacgag aagtccgtgt atatgcgggc agttccggga ttgtaattac ccgtgccaag
     1441 atgaacgtag cgcctgagcc cgtggccctc ccgcctgacg acgaggctga tctttgcgtg
     1501 cgtcttgagg ccgacgatgc cgtaggcgac gtgtactccc cgcgcctcga gctggcgggc
     1561 ccaggtgatg ttcggctcct cgtcgaagcg cgcctttaac tcgaccagga ccgcaacttg
     1621 cgtctgctcg tcacgtgcct cggagagctt ctcgactatc ggggagttcg agccgacgcg
     1681 gtagagcgtc tgctttatgg cgagcacctc ggggtcgctg gccgctgccc tcacgaagtc
     1741 gacgacgggg gcgaacgagt cgtagggatg gtagagaagt atgtcgccgt tgcggatcgc
     1801 ctgggtgata gagcgggcgc tccggatctc ggaaggcaca cggggcgtga tcgggggata
     1861 tagcaggtcc gagcgctcga ggtgcgtgag ctcctccaga tcggcgagcc ctatgggatg
     1921 tggcgcggcg aagaccgagt tctcgttgag ctttaggttg cctgcaagcc attcccgcag
     1981 gtcgtccggc atctcgtccg acacaacgat cctgacgctc tggccgaacc agcggctctc
     2041 gagctcgtcc tctatggcct gaaggaggtc agaagcctcg tcctcctcga tcacgaagtc
     2101 cgcgttgcgc gtaacctgga agacgtagc
//
LOCUS       DATAIR010000798         8690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_272138, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000798 DATAIR010000000
VERSION     DATAIR010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272138"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..764
                     /locus_tag="VHM69_18950"
     CDS             282..764
                     /locus_tag="VHM69_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742525.1"
                     /translation="MVEWALSHQGAVFSGEVVDVEDGPPIKMPSMGGIRLPSSTVTLR
                     VSEVWKGPQQETLEMSTPSAGAICGYPFKEGQDYLVYAYGKGEPFKVDLCSGTKPLSK
                     ANADLALLGKLGEGEKLKDSGDALNDTSGVVPARATVLIAVLAMAASFLLVVRLVRTG
                     "
     gene            complement(959..2059)
                     /gene="phaC"
                     /locus_tag="VHM69_18955"
     CDS             complement(959..2059)
                     /gene="phaC"
                     /locus_tag="VHM69_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEX2742526.1"
                     /translation="MTDTANYGAVPTSGDAAIPADVENFFDKYSRGMKIVVEGAQADT
                     GQTPKETVWTKNKAKLYHYAPNAEKKHPVPILMVYALINRPYILDLMPGNSFIEYLVN
                     EGFDVYLLDWGVPGDEDKDLDFENYVLDYIPRAVKKVLRHSHSEEFTMLGYCMGGTMS
                     AMYASLFPEHLKNLILMTAPIEFPEGEMGLYSLFTNEKYMNPGLLADAFGNIPGDLID
                     TGNRMLKPVTNYVGTYVNMWERIFEEKPMETWLAMNKWVNDGPPFPGATFRQWISEFY
                     QQNKLAKGEIVLRGRRVDLSKIEIPLLNIAGKKDHICTLPQAEATMNLVGSQDKEFFV
                     LDAGHVGLMTGRGAKKGLWPKVSDWLSTRSGA"
     gene            complement(2060..2989)
                     /locus_tag="VHM69_18960"
     CDS             complement(2060..2989)
                     /locus_tag="VHM69_18960"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2742527.1"
                     /translation="MTQERGVVSGMDPADLWRQWMESGSRMWSDMTRDAQEGQVDPYS
                     VYRQWFESMREMQERMIGAVPEPVTGNPGVQELWQRWFEMTSDSWRKSVELNQAAMEI
                     LPRWMQMLDEARTNLMAGGAPPSDPLQLTSQWYNATSGPYSDFVGDVIEREEFLEPSS
                     RFLQHYASFYKVFKRNSEEYLKSLQLPVRSDISRIAGLVVNLEDKVDRIEEVLEDFEY
                     GYAEPATAESVKALEGRLDRVEGKLDRLIAAIESGAAQTAEESTGTNGSNIKATDAAR
                     RKAREMGVNLAEVVGTGADGQVTVDDVRKKGES"
     gene            complement(3157..3573)
                     /locus_tag="VHM69_18965"
     CDS             complement(3157..3573)
                     /locus_tag="VHM69_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742528.1"
                     /translation="MLTPGEGPATVAILGGNPVIGKALESLLRSADYVAHFFSEYPED
                     DAEPLGDASVALVLPAVSSRHQESLIALIRNTPSTTNLPVIELITTPNEKGNGLVTRV
                     PWPCGIEDLRRFIDEKLSEDPLDSQGEPRSVDEIAS"
     gene            complement(3701..4402)
                     /locus_tag="VHM69_18970"
     CDS             complement(3701..4402)
                     /locus_tag="VHM69_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742529.1"
                     /translation="MQDESPDTKRLVLADDHHLLRRGFKSLLSSEPGLEVVGEASNGR
                     EAIEICLRLKPDLVLMDVRMPEMDGITATRQIKEEQPGVGVLMVTMHENPDYLLQALD
                     AGAAGYVLKDAPADRLISAVHRTLNGESPLNQELATRLLRQLAEEKQQKPYSRPSKRH
                     ESTGEVLTPRETEVLTLLTTGQTNQQIAQSLTISKGTAKVHVERIIRKLGVSDRTQAA
                     VRAIELGFTSPDTAR"
     gene            complement(4392..7082)
                     /locus_tag="VHM69_18975"
     CDS             complement(4392..7082)
                     /locus_tag="VHM69_18975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2742530.1"
                     /translation="MRRTSPLLRYGVAVLAVEVALVIKLLLDPLITQDTPFLLVFGAI
                     MVSAWYGGLGPGLLATVVAGLVTDYFFLPPERSFSGFSVEAVPLLAFVLEGTLLCLLT
                     EALRAARWRAENSKLEAERHHEDLRRSEEHFRSLVEGVRDYAIFMLDPQGRVASWNAG
                     AERLIGYKAEEIVGEQFTTFFIDEDPGQRRPERLLEEATREGRSEEESWRVRKDGSSF
                     WANSIITALYDDEGRQRGFSKVTQDITERKKSEELLKEAENRLRTLVEHVPAITYTEE
                     VGGHAMVYVSPQIEKVLGYSPREVMADPDLWTKMLHPHDREQVLDAERHSNETGDPFA
                     VEYRIFARNGRVVWLRDEAVLVRDEEGEPLHWQGFMLDVTERKRAEEKRRESDELYRN
                     VVERAAENIFLLDPFTKHILQANASFHNSLGYEPEELRRLTIYDIVSQVPENIDRNIQ
                     RVLEDGSHFIGERRYRRKDGSLIEVEVSAGALSYDGRPALCVVAHDVTERKSAEAALR
                     RSLDALLALYEAGQVLSSSLEREQIGSRLLEIVGRISGTTAAVIALRDESGELHTWRD
                     FGPDDLLRHIHEAPEAMRVRSAAFESGERGSFGLQVGGLKIGILAGLSMPMRARDQVI
                     GVLEVYGPKWLVESETADTFASLANQAASALENARFYEELTGRERQLHDLVVQLLAVQ
                     EEERRRVAYDIHDGLAQTAAAAHHHLQAFARHHPPGSAQGREDLDEALELVRSVVGEA
                     RQLIHDLRPTVLDDFGLAAAVRLQVDALKSEGWDVGLEDELGDERLPPEVETTLFRVA
                     QEALTNVRKHTQTTRVRVELDRSGRAVRLLVRDEGHGFQPDDATRNNGPGERVGLSGM
                     RERVSLLGGRFDLHSEPGSGTTVTAEVELPVTREDADHAG"
     gene            complement(7426..>8690)
                     /locus_tag="VHM69_18980"
     CDS             complement(7426..>8690)
                     /locus_tag="VHM69_18980"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2742531.1"
                     /translation="TVSLTISAVNDAPVANGQSVSTNEDVSKDVTLSASDIENDNLGY
                     SIVNSPTHGSLSGTGANRTYTPDADNNGPDSFTYKANDGTADSNVATVNITVDSVNDK
                     PVISSVTATGTPVDEGSSASIKVTASDPDTPAADLRYSFDCNGDGDYVDAGDKGPQAA
                     DSASCAFDDGPGANPYKVNVRVTDGAGGSAADFTNVGVRNVAPTITDISASPQLALLN
                     QSINFTGTATDPSVADTNAGFTWKWSKDGVVQTNMTTNILQTSFNTCGPHTVSATATD
                     KDGGVSPLVTKSNVVNVLESSFRPPIDGPATNLTLKGKVIPVKIYAGCNNVVQPGLTP
                     NIKLLNGDVRAGQEGAGDVVEAYSTSAADSTGWMRPVDGGYIYNLQVPGGVTVAVGQE
                     FTIRVYPLATSANPTAGPPMYAVLKIKK"
BASE COUNT         1439 a         2709 c         2686 g         1856 t
ORIGIN      
        1 gcactcgggt gaatgagcct gccagttgcc ctgtgcaccc gcttgccacc gattgtagcg
       61 acaaagatct tctattgggg gatgcgccaa tccgcacaaa cccctaaact gcttttgtgg
      121 tcggggcgtc cgaccgccac acgcagaagg agcatgtgtc catgagtagt cgactgattg
      181 gcgctctggc ctccttagtc ctcacgatcc ttgggcttgt gctcctgctt cccgactgcg
      241 cctccgcctg ctcttgtggg ggtggcggta gcgataaaca gatggtcgag tgggcgctat
      301 ctcatcaggg cgcagtgttc tcaggagagg tggtggatgt cgaagacgga ccgcccatca
      361 aaatgccatc aatgggggga attcgtttac cctcatccac ggtaaccctg cgggtctctg
      421 aggtgtggaa gggtccacag caagaaacgt tggagatgag cacgccgagc gctggggcga
      481 tctgcgggta ccccttcaag gaagggcaag attacctggt ctatgcctac gggaaggggg
      541 aaccgtttaa ggtggatctc tgcagcggga ccaagccgct ttcgaaggcg aatgcagacc
      601 tcgcattgct cgggaagctc ggggagggcg agaagctaaa ggacagcgga gacgccctca
      661 acgacacctc tggggtcgtt ccggcgcgtg cgacggtatt gattgccgtg ctggcgatgg
      721 cggcgtcatt cttgctcgtg gtgcggcttg tgcgcaccgg ataggtcgtc ctctccgagc
      781 tttgcctcct ctgacgctct gcatagagga gtgtatggat gcgccgtggt tccgggtagt
      841 gaagcaagtc gtgagttcta catgacagga tgtcatacga cgaaccgcag aggcgcggaa
      901 agcgtagagg ggacggccat aatggccgtc ccctccgggg tgctcgaagt tgttggtgct
      961 atgcgccgga gcgggtgctc agccagtcgc tcaccttggg ccagaggccc ttcttggcgc
     1021 cgcggcctgt catgaggccg acatggcctg cgtcgaggac gaagaactcc ttgtcctggc
     1081 tgccgacgag gttcatcgtc gcctctgcct gcgggagggt gcagatatgg tccttcttgc
     1141 cggcgatatt gagtagtggt atctctatct tcgagaggtc cacgcggcgg ccacgcagga
     1201 cgatctcgcc ctttgccagc ttgttctgct ggtagaactc cgagatccac tgcctgaacg
     1261 tcgcgccggg gaatggggga ccgtcgttga cccacttgtt catggcaagc caggtctcca
     1321 tcggcttctc ctcgaagatg cgctcccaca tgttgacgta ggtgccgacg tagttggtga
     1381 cgggcttgag catcctgttg cccgtgtcga tgaggtcgcc ggggatgttg ccgaaggcgt
     1441 cggcgagcaa cccagggttc atgtacttct cattcgtaaa gaggctgtag aggcccatct
     1501 cgccctcggg gaactctatg ggggcagtca tgaggatgag gttcttgagg tgctcgggga
     1561 agagtgaagc gtacatggcg ctcatcgtgc cgcccatgca gtagccgagc atggtgaact
     1621 cttcggagtg cgagtgcctc aggaccttct ttacagcccg cggaatatag tcgaggacgt
     1681 agttctcgaa gtcgaggtcc ttgtcctcgt cccctgggac gccccagtcg agcaggtaca
     1741 catcgaagcc ctcgttcaca aggtactcga taaagctgtt gccgggcatg aggtcgagga
     1801 tgtagggcct gttgatgagg gcgtagacca tgaggatagg gacgggatgc ttcttctccg
     1861 cgttcggcgc gtagtggtag agcttcgcct tgttcttggt ccagaccgtc tcctttggcg
     1921 tctggccagt gtcggcctgg gcgccctcga cgacgatctt catcccacgg ctgtacttgt
     1981 cgaagaagtt ctcaacgtct gcgggtatgg ctgcgtcgcc cgaggtaggg accgcgccgt
     2041 agttggccgt gtcggtcatc tagctctctc ccttcttgcg cacgtcgtcg acggtgacct
     2101 ggccgtccgc gccggtccct accacctcgg cgaggttcac gcccatctca cgcgccttgc
     2161 gccttgccgc gtccgtggcc ttgatgttgg acccgttggt gcctgtgctt tcctcagccg
     2221 tctgcgcggc accgctctcg atcgccgcga tcaaacggtc gagcttgccc tctacgcggt
     2281 cgagccgtcc ctccagagcc ttgacggact cggcggtagc cggctcggcg taaccgtatt
     2341 cgaagtcttc caggacctct tcgatgcggt cgaccttgtc ctccaggttg acgacgaggc
     2401 cggcgatcct tgagatgtcc gaacgcacag gcagctggag gctcttgagg tactcctcgg
     2461 agttgcgctt gaagaccttg tagaagctgg cgtagtgctg gaggaagcgg ctcgacggct
     2521 ccaggaactc ctcgcgctcg atgacgtccc cgacgaagtc cgagtagggg ccgctcgtgg
     2581 cgttgtacca ctgggacgtg agctgcaatg ggtcgctcgg cggcgcgccg cctgccatca
     2641 agttggtccg cgcctcgtcg agcatctgca tccatcgcgg caggatctcc atcgccgcct
     2701 ggttcaactc cacgctcttg cgccacgagt ccgaggtcat ctcgaaccac cgctgccaca
     2761 gttcctgaac cccagggttg cccgtgacag gctccgggac cgccccgatc atccgctcct
     2821 gcatctcgcg catactttca aaccactggc ggtatacgct gtaggggtcc acctggccct
     2881 cctgggcgtc gcgcgtcatg tccgaccaca tcctgctccc cgactccatc cactggcgcc
     2941 agaggtcggc agggtccatg ccggacacta ctcctctctc ttgcgtcaac cgaaacctcc
     3001 tcgtctgccc tgcaccgata ctatgcacgg cgctaccctc atcaaggcca acgccgatca
     3061 cgcgaattat actagcaaac gagacttatg tgttaacggc atataccgtt ggtgaattgc
     3121 cctggtctct acggattgag gcgggaagtc tgggtgctag ctcgcgatct cgtcgacgga
     3181 tcgcggttcg ccctgagaat cgagcgggtc ttccgagagc ttctcatcaa tgaagcgccg
     3241 caggtcctct atgccgcagg gccagggcac cctggtgact aggccgttcc ccttctcgtt
     3301 cggggtcgtt atcagctcga tcacgggcag gttagtcgtc gatggggtgt tcctgatcag
     3361 ggctatgaga gattcttgat gcctgctact cacggccggt aggacgagcg ccacgctcgc
     3421 gtctccgagc ggctcggcgt cgtcttcggg gtactcggag aagaagtgcg ccacatagtc
     3481 ggcggagcgg agcagcgatt ccagggcctt cccgatcacg gggttgccgc cgaggatggc
     3541 gacggtagcg ggcccttctc ccggggtcag catgctcgtg acagatccgt acccatggcc
     3601 attcgctgat tatatggtgg tagtgggcgt ccgcgcgtac gccgtgtggc ctattttgcg
     3661 tatgccacct ggcatatttg ccggatcgcc tgggagtgct ctagcgcgct gtgtcgggag
     3721 acgtgaaccc cagttcgatg gcgcgcacgg cggcctgggt acggtccgag acccccagct
     3781 tgcggataat acgctcaacg tgcaccttgg ccgttccctt gcttatggtc aaggattgcg
     3841 cgatctgctg gttcgtctgc cctgttgtca ggagggtgag gacttccgtc tcgcgcggcg
     3901 tcagcacttc acccgtggac tcgtggcgct tcgaagggcg cgaatacggc ttctgctgtt
     3961 tctcttccgc cagctgcctg aggagtcgtg tggccagttc ctgattgagg ggagactcgc
     4021 cgttgagcgt tctgtggacc gcgctgatca ggcggtccgc gggggcatcc ttgagcacgt
     4081 agccggcggc gccggcgtcc agtgcttgca gcaggtagtc cgggttctcg tgcatggtca
     4141 ccataaggac gcctacgccc ggctgctctt ccttgatctg gcgggtcgcc gtaatgccgt
     4201 ccatctcagg catccgcacg tccatcagca ccaggtcggg cttcagacgc aggcagattt
     4261 cgattgcctc cctgccgttc gacgcctcgc ctactacctc cagccccggc tcgctgctca
     4321 acaggctctt gaaccctcgc ctgaggaggt ggtggtcgtc ggcgaggacc agccgcttgg
     4381 tatccgggct ctcatcctgc atgatcggcg tcctccctgg tcactggcag ttcaacctcg
     4441 gccgttaccg tcgtacctga ccccggctcg ctgtggaggt cgaacctccc tccgagtaag
     4501 gatactctct cgcgcatccc ggagagacca accctctcgc cggggccgtt attcctggtc
     4561 gcatcatcgg gttggaaacc atgcccctcg tctctgacga ggaggcggac cgccctgccc
     4621 gagcggtcga gctccacgcg tactctggtg gtctgcgtgt gcttgcgaac gttcgtaagg
     4681 gcctcctggg cgaccctgaa cagggtcgtt tcaacctccg gtggcagtcg ctcatcgcca
     4741 agctcatctt cgaggccgac gtcccaaccc tccgacttga gcgcatcgac ctgcagtcgg
     4801 acggcggcgg ccaggccgaa gtcgtcaagc accgttgggc gcaggtcgtg gatcaactgc
     4861 cgcgcctccc cgacgacgct tcttactagc tccagcgctt catcgaggtc ctcccggcct
     4921 tgcgccgagc ccggaggatg gtgacgagcg aaggcctgaa ggtgatggtg ggccgccgcc
     4981 gccgtctgcg ccaggccgtc gtggatatcg taggcgacgc gacgccgctc ctcttcctga
     5041 accgcgagga gctggacgac gaggtcgtgc aactggcgct cccgtccggt tagctcctcg
     5101 tagaacctgg cgttctcgag cgcgctggcc gcctggttcg ccaggctcgc gaacgtgtct
     5161 gccgtctcgc tctcgaccag ccacttggga ccgtagacct cgagcacccc gataacctgg
     5221 tctcgcgccc gcataggcat ggagagaccc gcaagtatgc caatcttcag gccgcctacc
     5281 tgcagcccga acgaccctct ctcgcctgac tcgaaggccg cgctgcgcac cctcatggct
     5341 tcaggcgctt cgtggatgtg gcggaggaga tcgtcggggc cgaagtcgcg ccacgtgtgc
     5401 aactctccac tctcgtcacg caaggctatt accgccgccg ttgtgcccga gattcggccc
     5461 acgatctcga gcaacctgga accgatctgc tcccgctcga ggctggagct cagaacctgc
     5521 cccgcttcgt agagcgcgag aagcgcgtcc aggctgcgcc ttagcgccgc ctcggcgctc
     5581 ttgcgctcgg tcacgtcgtg ggctacgacg caaagcgctg gcctgccgtc gtaggagagt
     5641 gcgccagcgc tgacctccac ctcgatgagt gagccgtcct tgcgcctgta ccgcctctcc
     5701 ccgataaagt ggctcccatc ctcgagaacg cgctggatgt tgcggtcgat gttctcgggg
     5761 acttgcgaga cgatgtcgta gatcgtcagg cggcgaagct cctcgggctc gtaaccaagc
     5821 gaattgtgga acgaggcgtt cgcctgaagt atatgtttgg tgaaggggtc gagtaggaag
     5881 atgttctcgg ccgcccgctc gacgacgttt cggtagagct catcgctctc gcgccgcttc
     5941 tcctcagccc gctttcgctc ggtaacatcg agcatgaagc cctgccagtg gaggggctct
     6001 ccctcttcgt cgcgcaccag caccgcctcg tcgcgcagcc agaccacgcg cccgttcctg
     6061 gcgaatatcc tgtattcaac cgcgaaggga tctccggtct cgttgctgtg cctctccgcg
     6121 tccagcacct gttccctgtc gtggggatgc aacattttcg tccagagatc aggatcggcc
     6181 atcacctccc gtggggaata gccgagcacc ttttcgatct gcgggctgac gtacaccatg
     6241 gcatggccgc ctacctcttc cgtgtatgtg atcgccggca cgtgttcgac gagcgtgcgc
     6301 aggcggttct ctgcctcctt gagcagctcc tcggatttct ttcgctccgt gatgtcctgt
     6361 gtgaccttcg agaaaccccg ttggcggccc tcatcgtcgt acaaagccgt tatgatcgag
     6421 ttggcccaga acgaagaacc atcctttctc acgcgccaac tctcctcctc ggaccggcct
     6481 tctcgtgtcg cctcctccag cagtctctcc ggcctgcgct gccccgggtc ctcatcgatg
     6541 aagaacgtcg taaattgctc gcccacgatc tcctcggcct tgtatcctat gagacgttcc
     6601 gccccggcgt tccaactggc gacgcgcccc tgcgggtcga gcatgaagat cgcgtagtca
     6661 cgtacgcctt ccacgagcga gcggaagtgt tcctcgctcc ggcgcaggtc ttcgtggtgg
     6721 cgctcggcct ccagcttgct attctcggcc cgccacctgg ctgcgcgcag cgcctcggta
     6781 agcaagcaga ggagcgtacc ttcaaggacg aacgcgagaa gcggcacggc ttccacgctg
     6841 aaccccgaga aggatctctc cggggggagg aaaaagtagt ctgtgaccag tcccgccacc
     6901 acggtcgcga gcagtcccgg tccaagtccc ccgtaccagg cgctcaccat tatcgcgccg
     6961 aagaccagga ggaaaggagt gtcctgcgtg atcagcgggt ccagcaacag cttgatcaca
     7021 agcgccacct cgaccgcgag cacggcgacc ccatagcgca ggagtgggga agtgcgtctc
     7081 atcgagagca tctaaacctg ataccccccg gacccagcct ggactgcgga tgtctcggcg
     7141 cgcactttcc taatcctctt atcgtaccta tcgagatcta acagagacat tgtgagcctg
     7201 tgtaatggtt ctcgcctccc ccaaacgtac tattttcgtg aagctttttc tacgctgcct
     7261 ggtaactttt tgatccacct gacgaggaac atgagatcac tttggacctt cctgcagaag
     7321 cccgtgaagc gagtacggtg ttcgaaggtg ccaaaggaaa gggccagagc gttaagctcc
     7381 ggccctccga ttctcgggct atggatcagc ccgtccattc acctcctact tcttgatctt
     7441 gaggacggcg tacattggcg gtcctgcggt cgggttcgcc gaggtggcga gtgggtagac
     7501 cctgatggtg aactcctggc cgacggccac cgtcacgccg ccaggaacct gcaggttgta
     7561 gatgtatcct ccatcgactg ggcgcatcca gcccgtgctg tcggccgcgg aggtggagta
     7621 cgcctcgacg acgtctcccg ccccttcctg tccggcccgc acgtcaccgt tgagaagctt
     7681 gatgttgggc gtcaggccag gctggacgac attgttgcac cctgcgtaga tcttgacggg
     7741 tatgaccttg cccttgagtg tcaggttggt cgccggcccg tcgatcggcg gcctgaagct
     7801 cgactcgagc acgttcacca cattggactt ggtgaccagg ggcgacactc cgccgtcctt
     7861 gtcggtggcc gtggcgctca cggtgtgcgg tccgcaggtg ttgaagctgg tctggaggat
     7921 attggtggtc atattggtct gcacgacccc gtccttggac catttccagg tgaagccggc
     7981 gttcgtatca gccacgctcg gatcggtggc cgtgccggtg aagttgatgc tctggttcaa
     8041 caatgccagc tgcggcgagg ccgagatgtc ggtgatcgtg ggcgccacgt tcctgacgcc
     8101 gacgttggtg aagtccgcag ccgagccgcc ggctccgtcg gtcaccctga cgttgacctt
     8161 gtaaggattg gcgccggggc cgtcatcgaa ggcgcacgag gcgctgtcag cggcctgcgg
     8221 tcctttgtcg cctgcgtcta cgtagtcccc gtcgccgttg cagtcgaagc tgtagcgcag
     8281 atcagcggcc ggggtgtccg gatcgctggc cgtcaccttt atggacgcgg aggagccctc
     8341 gtcaaccggg gtgcctgtag ccgtgaccga cgagatcacc ggcttatcgt tgaccgaatc
     8401 aacagtgatg ttcaccgtgg cgacgttgga gtcggcggtg ccgtcattgg ccttgtaggt
     8461 gaaggagtca ggaccgttat tgtcggcatc cggggtgtag gtacggttag cacccgtgcc
     8521 actgaggctg ccgtgggtcg gactattaac gatggagtag cccaggttgt cgttctcgat
     8581 gtcagaggca ctgagggtaa cgtccttgga gacgtcctcg ttggtactaa ctgattgccc
     8641 attagcgaca ggagcgtcat tcacagcgct gatcgtgagg ctaactgtag
//
LOCUS       DATAIR010000799         9717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_228412, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000799 DATAIR010000000
VERSION     DATAIR010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228412"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VHM69_18985"
     CDS             complement(<1..474)
                     /locus_tag="VHM69_18985"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742532.1"
                     /translation="MSETEKITINLGLVDLGQIDLLVQEGFYSNRTDFIRTAIRNQLT
                     THAEEVRRTVARKTLVLGLQNYTRRDLEAVREAGETLEIKVLGLASIDDDVDPELALA
                     TIESINVLGALRAPSTLMDSMVARASSGSTSSSMLARPRTLISRVSPASRTASRSR"
     gene            697..1659
                     /locus_tag="VHM69_18990"
     CDS             697..1659
                     /locus_tag="VHM69_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HEX2742533.1"
                     /translation="MNVLVSGASGFIGSALIPELEAGGHRAIRLSRSKPSTEDTIRWN
                     PDSGELDPSRLEGVDAVVHLAAESVGEGRWTPEKKRRIMDSRREGTRLLSETIAGLSE
                     PPRVMVSVSGINYYGSRGNELLREESGPGDTFLARVCQEWEGAADPARRAGIRVVHPR
                     FGLVFPQGFSRNLLGLTVGIQHPRFEITFGTRGSTRARLPVYKLTGGGWIGSGRQFWS
                     WLVAEDVVGVILHALTTDSLEGPVNAVSSSVTAREYTTTLNRVLNRPTVFPLPAPLAR
                     LALGDFAEEMILASIRVDSTRLMESGYEFRYPELEGAFRHLLGR"
     gene            complement(1663..2709)
                     /locus_tag="VHM69_18995"
     CDS             complement(1663..2709)
                     /locus_tag="VHM69_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX2742534.1"
                     /translation="MSRTLGIIGVGLIGGSVGLAARDSGWEVLGVDRPEVLEEAAQSN
                     AIHRSSTLKEVRETDLVVLAAPISKVTGLVEDLTPTDALITDVASAKSAVVRAAESQG
                     LRFVGGHPMAGSQLSGVANARANLFHGARYFLTPTDNTNPEDYREVAGFVRELGAVPT
                     AVEPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFRDLTRVGASNPELW
                     SDILAENAPALGEALGAFAGAMAQLGSEIKDREVMEDRFRQAREAYDALGGILVEKSG
                     ENVEVAIPVENRPGVFAEVTTLMGSNNINILDLYVRHSNTERAALVLTLDAGDAPEAR
                     EMLREAGFWAEIQS"
     gene            complement(2706..3773)
                     /locus_tag="VHM69_19000"
     CDS             complement(2706..3773)
                     /locus_tag="VHM69_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2742535.1"
                     /translation="MPVTFRPELDPLSPYKPPRAAFEAEAERGADGYVKLTSNELSFG
                     PLPEAEAALAEALPRANRYPDRYAASLREAVVSANPGSGFENILVGNGSSEILLNLLQ
                     LVERPGEVIFPWPSFSLYASIGTILGLKTTKVPLTERHEMKPEALLSAVTGETRAIIV
                     SNPNNPTGTYLTLEEVRSFADDLPDNVLLILDEAYQEFVSDPAYSGSHELALERENVV
                     CVRTFSKAHGLAGFRVGYGIARQRVADYVERVRFPFSVNLAAQVAAAASMRARDKIEA
                     RARFVMRERDRIETAFRKAGLNYVPSQGNFVLVETGPEVFERGGVLVREGDPLGYPGW
                     SRVTVGDSGENDRVIGALGGA"
     gene            3972..4253
                     /locus_tag="VHM69_19005"
     CDS             3972..4253
                     /locus_tag="VHM69_19005"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2742536.1"
                     /translation="MQQKVRELRERVDEVDSELIRALSERARIVQEIMVLKAEAGAPV
                     YDPKREEEILRSVVERNPGPIYDSSMRDIFELILHRIRDLEIQRGEFSR"
     gene            4255..5067
                     /gene="aroF"
                     /locus_tag="VHM69_19010"
     CDS             4255..5067
                     /gene="aroF"
                     /locus_tag="VHM69_19010"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX2742537.1"
                     /translation="MDVSTGVKTDVKTKNGMIQPGTFRVIAGPCTVESYEQFVASANA
                     VKQAGFSYVRGGAFKPRTSPYSFQGLGEEGLKILSEVSRGLGLKVVTEVMDPYDIDLV
                     MEHAEILQIGARNMANFSLLRRVGAAIAGTDHGVVLKRGLSATVEEWLLAAEYVTAAG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     AVAAEADGLMVESHHEPQEALCDGEQALPVETLMGMKDVLQPFASAMGREVI"
     gene            5076..6701
                     /locus_tag="VHM69_19015"
     CDS             5076..6701
                     /locus_tag="VHM69_19015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2742538.1"
                     /translation="MDPASSVRRLGDRFVLVRELGFGGMARVFLGRDEVLDRPVAVKV
                     VEPDAEDPEIGLRFQREGRAAARLSHPNIVRVFDAGEDKLEGRAVSYIVMEYVPGGDL
                     QDLMDRNGPLPETMLSRVGADVAAGLAHAHARGIVHRDVKPRNILLDDRGSPKLADFG
                     IARALDATTSQNRAGSYLGTAAYSSPEQLRGERVTSKSDVYSFGATLYHAAVGKRPFS
                     GNSIEVANQHVLKMPTPPRERGARIGEDLEALILGCLAKNPAKRPDAARLSDKLQQRS
                     AADATAALVGSAARGRATGVSRVAGAAKSAGSSGVGAVRQGFARTRASGPPDSTISLP
                     TRTFRAGSRQRTMLAILMVALLLLILTVAGAWALLGQGEQGNNTPGKDVGRQEQAGAQ
                     KQEPKENPKPDEAAAPKPSGESRPEGGAPEENPAPAPPLAEAEKAVYDLYYQESFARV
                     GASWAFLSQRLQNEIGSPERWAEQEDIYTFTYMEFISYPVARAVGEEAEVTFEVRLDH
                     TWGSELLSGTWVCVNEGGEWKLDRLENERTVPA"
     gene            6783..8255
                     /locus_tag="VHM69_19020"
     CDS             6783..8255
                     /locus_tag="VHM69_19020"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2742539.1"
                     /translation="MESELGSGGMARVFLGRDEVLDRAVAVKLLNPVHGGTDIGDRFE
                     REGRTAARLAHPNIVQVYDAGEAEFNGRETSFIVMEYVPGGDLKALIDQKGRLPGSEL
                     ARLGDEVCAGLAHAHEHGVIHRDIKPHNILLDENGHAKVTDFGIARALDATQATRTGY
                     FLGTAMYSSPEQLQGQKVTPKSDVYSLGTTLYQAATGETPFAGDTPIEVASQHVSKTP
                     TPPRQRGADVSEDLAALILSCLAKNPDDRPTAEEARSRLREAIPTAGATQAQPAAPTR
                     AEQTRPAPGDGQGRVSREDRRGRRGGLLAVLALFAVLVVIGALAAQNFMGGGQNPGSN
                     GAGQKVAQGGNEGQSDGEPGGNGSQGGGQQSAATSSAESAPSQDNSGKGDQGTLTAQA
                     AEQTIRDFYKTAAGSDYESAWDFLTPRYKRDIGSRGNLTNQFRTLESIEFTDGPTAEV
                     NGDTAEVSFSTIARHEDRVDRPTLTATLVKVDGEWKIDDL"
     gene            8417..9472
                     /locus_tag="VHM69_19025"
     CDS             8417..9472
                     /locus_tag="VHM69_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2742540.1"
                     /translation="MTIKEVAPQNPSGVTPVAADGSSVREVYTRDGRGVVSVDVAATS
                     EGGPMGGSGFVIDEAGHIVTNQHVVEGAEDISVRFADGARREAEIVGQDPSTDVALIK
                     VDAPREALQPLTLGDSNSVGVGEPVIAIGNPLNVGISVTTGIVSGLGRPIMAPNNYTI
                     NDAVQTDAAINPGNSGGPLLDSRGTVIGVNAQIASESGGFEGVGFAVPIDTVKSVVKQ
                     LITEGKVVHGYIGVSMFELGIDELSAYTGLSEESLREQYGFPGNGAIVSEVTPDGPAE
                     KAGIRGGEEKDIEGIAVPIGDVITEIEGKPISTSEEVIEVVNSLKPGERLPMTVVTPG
                     EDERSVEVKVGTRPEGV"
     gene            9476..>9717
                     /locus_tag="VHM69_19030"
     CDS             9476..>9717
                     /locus_tag="VHM69_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEX2742541.1"
                     /translation="MSGDELKREAAFAAVERYVESGMVVGLGTGSTASFAVRRIGELM
                     ASGELRDIKGVPTSARTTALANEMGIPLATLAEARPR"
BASE COUNT         1840 a         2911 c         3270 g         1696 t
ORIGIN      
        1 ccgcgacctg gaggcggtgc gcgaggctgg tgagaccctg gagatcaaag ttctgggcct
       61 ggcgagcatt gacgatgacg tagatcctga gctggccctt gcgaccatag agtccatcaa
      121 cgtgctcggc gccctcaggg ctccaagcac gttgatggat tctatggtcg caagggccaa
      181 ctcaggatct acgtcgtcgt caatgcttgc caggcctaga actttgatct ccagggtctc
      241 accagcctcg cgcaccgcct ccaggtcgcg gcgagtgtag ttttgcagtc cgagcaccag
      301 cgtcttgcgc gccaccgtcc gcctgacctc ctcggcgtgc gtcgtgagct ggttgcggat
      361 ggcagtccgg atgaagtcgg tgcggttgga gtagaagccc tcctgtacca gcagatctat
      421 ctgccccaga tccacaagcc ccagattgat cgtgatcttc tccgtctcgc tcatgccagc
      481 acctcattcg ccgtacatct gctcaacatc catataccat ccacatggat ggtacaagat
      541 aggtcatgac tttgtcaagt aggagttttg tgggctttgg tggcctgggc gggtggggtg
      601 cgggagtacg tatttatgtg agtgagctta tacggactgg tgggagcgcg ggcgttacca
      661 taggagcaaa gtagatatcg ggtttggagg aagatcatga acgttctcgt gagtggggct
      721 tcggggttca tcgggtcggc tcttatccct gaactggagg ctggcgggca cagggcgatc
      781 cggcttagcc gttcgaagcc ttctacggag gacactattc gctggaaccc tgactcgggt
      841 gagctcgatc cctcgcgtct ggaaggtgtg gacgccgtcg tacacctggc cgcggagagc
      901 gtcggggagg ggcggtggac gcctgagaag aagcgacgaa tcatggacag ccggagagag
      961 ggaacccgcc tactctccga gaccatcgcc ggactctctg agccgcctcg agtaatggtc
     1021 tctgtctcgg gcatcaacta ctacgggagc agaggaaacg agctcttgcg tgaggagagc
     1081 ggtcctggag acaccttcct cgcccgggtg tgtcaggagt gggagggggc ggcggacccg
     1141 gcgcggaggg ccggcatccg ggttgtgcat cccaggttcg gtctagtgtt cccccagggg
     1201 ttttcccgga acctcttagg tcttacggtg gggatccagc atcccaggtt cgagatcact
     1261 ttcgggacca gaggttccac acgcgccagg ttacctgtct acaagcttac ggggggtgga
     1321 tggatcgggt cgggcaggca gttctggagt tggctcgtcg ctgaggatgt ggttggcgtg
     1381 atcctacacg cccttactac cgactccttg gaaggtcccg tcaatgcggt gtcgagctca
     1441 gtcacagctc gcgaatacac tacgaccctc aaccgcgtct tgaatcgccc gaccgtcttc
     1501 ccgcttcccg cccccttggc ccgactagcc ttgggggatt tcgctgagga gatgatcctg
     1561 gccagcatcc gtgtcgactc caccaggctc atggagtcag gctacgagtt ccgctatccc
     1621 gagctcgaag gagcctttcg acacctgctc gggaggtagt ctctagctct gaatctccgc
     1681 ccagaagcct gcttcgcgta gcatctcgcg agcttctggg gcgtccccgg catccagcgt
     1741 gaggacgagg gccgcgcgtt ctgtgttgga gtgccggacg tagaggtcga ggatgtttat
     1801 gttgttcgag cccatcaggg tcgtcacctc ggcgaagacg ccgggacggt tctcgacggg
     1861 gatagcgacc tcgacgttct caccgctctt ctcgacgagg atgccgccga gggcgtcgta
     1921 ggcttcgcgg gcctgcctga agcggtcctc cataacctcg cggtctttga tctcactccc
     1981 gagctgcgcc atcgcgcccg cgaatgctcc aagggcttca ccgagcgcag gggcgttttc
     2041 ggctaggatg tccgaccaca gctccgggtt ggatgctccc accctcgtca ggtccctgaa
     2101 ggaggggccg gcgaaggaga gcgcctccgg agagatgtcc gaggcgacct tcaagagcgc
     2161 cgcggccatc aggtgcggca ggtgagagag ggcagccatc aggaggtcgt gtttctcggg
     2221 ctcgacggcg gtaggtacgg cgcccagttc cctgacgaac cccgcgacct cgcggtagtc
     2281 ctcaggattt gtgttgtccg tcggggtgag gaagtatcgg gcgccgtgga agaggttggc
     2341 gcgggcgttg gcgacacccg agagctggct gcccgccatc ggatgcccgc cgacgaagcg
     2401 tagtccctgg ctctcggccg cgcgcacgac ggcgctcttg gcgctcgcga cgtctgtgat
     2461 gagtgcatcg gtcggggtga ggtcctcgac gagtcccgtc accttcgaaa tgggtgcggc
     2521 gagtaccacg aggtccgttt cgcgtacctc tttcagcgtg gaggagcggt gtatggcgtt
     2581 gctctgcgcg gcctcttcca gaacctcggg acggtctacg cccaggacct cccatcccga
     2641 gtcgcgggcg gcgaggccga ctgagccccc gatcagtccg acaccgatga tcccgagcgt
     2701 acggctcacg cccctccgag ggctcctatg accctatcgt tctcaccgga gtctccgacc
     2761 gtcacccggc tccagccggg gtaaccgaga gggtcgcctt cccgcacgag gacgccgcca
     2821 cgctcgaaga cctccggtcc tgtctctacc aggacgaagt tgccctggga agggacgtag
     2881 ttcagtcctg ccttgcggaa agcggtttcg atcctgtctc gctcgcgcat cacgaaccgg
     2941 gcgcgggcct ctatcttgtc cctggcgcgc atcgaggctg cggcggcgac ctgggctgcg
     3001 aggttcacgg agaatgggaa gcggacccgc tcgacgtagt cggccaccct ctgacgtgct
     3061 atcccgtatc cgacacgaaa gccggcgagg ccgtgcgcct tcgagaacgt gcgcacgcag
     3121 accacgttct cccgctccag cgcgagctcg tgcgagccgc tgtaagcagg gtcggagacg
     3181 aactcctggt aggcttcgtc caggatcagg agcacgttgt ccgggaggtc gtcggcgaag
     3241 gaccgcacct cctcaagggt caggtacgtg cccgtcgggt tgttcgggtt cgagacgatg
     3301 atggcgcggg tctcgccggt cacggcggag agcagggcct cgggtttcat ctcatgccgc
     3361 tccgtgaggg gaaccttcgt ggtcttcaat ccaagaatgg tcccgataga agcgtacagg
     3421 ctgaaggacg gccaggggaa gatcacctct ccgggccgct cgacgagctg gaggaggttg
     3481 agcaggatct cactcgaccc gttgcccacc aggatgttct cgaagccgct cccggggttg
     3541 gccgaaacca cagcctcgcg caaagatgcc gcgtagcggt cgggatagcg gttcgcgcgg
     3601 ggtagagctt cggccagtgc cgcttcggcc tcggggagcg gaccgaatga gagctcgttc
     3661 gaggtcagct tcacgtagcc gtccgcgccg cgctcggcct cggcttcaaa tgcggcgcgc
     3721 ggtggcttgt aaggggacaa tgggtcaagt tcgggtctga aggttacagg caccaggtat
     3781 cagctttcag gtcgtggttg acgaatctct aaagactaag gccctttagc gggtcttgca
     3841 acaaaggcgg gcgacggact gatttcggca ttttacatat tttctgatgc ctgtagcctg
     3901 aaacctgatg cctacacctg atacctgtag cctgcgctgg tacaatcgca agtcctatgg
     3961 cggatactga gatgcagcag aaagtcagag agctcaggga gcgggtggat gaggtagaca
     4021 gtgaactcat ccgggcgctg agcgagaggg cgcgcatcgt gcaggagatc atggttctca
     4081 aggccgaggc gggcgcccca gtctacgacc ccaagcgcga ggaggagatc ctgcggagtg
     4141 tcgtggagcg caaccctggt ccgatctacg actcctcgat gagggacatc ttcgagctca
     4201 tcttgcatcg catccgcgac cttgagatcc aacgcgggga gttttccagg tagcatggac
     4261 gtttcgactg gcgtaaagac cgatgtaaag accaagaacg gcatgatcca gccaggcacc
     4321 ttcagggtta tagctggtcc atgcacggtc gagagttacg agcagttcgt cgcatccgct
     4381 aacgccgtga agcaggcggg cttcagctac gtccgtggcg gcgccttcaa accccgcacc
     4441 tcgccatact ctttccaggg actgggtgag gaaggactga agatcctctc agaagtctcg
     4501 agggggctgg gtcttaaggt cgttaccgag gtgatggacc cttacgacat cgatttggtc
     4561 atggagcacg cggagatact gcagatcggg gcccgcaaca tggcgaactt ctcgctgctc
     4621 aggcgcgtcg gcgcagcgat agcgggaacg gaccacggcg tcgtcctgaa aagagggctc
     4681 tcggcgacgg tcgaggagtg gctcctcgcc gccgagtacg tcacggcggc tggcggggac
     4741 aacgtggttc tttgcgagcg gggcattcgc acgttcgaga cgagcacgcg cttcacgctg
     4801 gatctctccg cggtcatcgt ggcgaaacgt ctgacggatc tgcccgtgat cgtagaccct
     4861 tcccacgccg ccggaaggcg cgacctcgtc gtacccctgt cgaaggccgc ggtcgcggcc
     4921 gaggccgacg gcctgatggt ggagagccac cacgagccgc aggaggcgct gtgtgatggg
     4981 gagcaggcgc tacctgtcga gacgctgatg gggatgaagg acgtgctgca acccttcgcc
     5041 agcgccatgg gcagggaggt catctagtct cggggatgga ccccgctagc tctgttcgac
     5101 gactcgggga caggttcgta ctcgtgcgcg aactgggctt tgggggcatg gcgcgcgtct
     5161 tcctcggccg tgacgaggtg ctcgaccgtc ccgtcgccgt gaaggtcgtc gaacccgacg
     5221 ccgaagaccc cgagatcggc ctccgattcc aacgcgaggg gcgggctgcc gccaggctct
     5281 cccaccccaa catcgtcagg gtcttcgacg ctggcgagga caagctcgaa ggacgcgcgg
     5341 tctcatacat cgtcatggag tacgtcccag gcggcgactt gcaggacttg atggacagga
     5401 acggaccgct ccctgagacc atgctctcgc gtgtcggggc cgacgtcgcc gcgggtctcg
     5461 cccacgccca cgcgcgcggc atcgttcaca gggacgtcaa accccgaaac atactccttg
     5521 atgacagggg gagtcccaag ctggcggact tcggcatagc ccgtgccctg gacgcgacga
     5581 cgagccaaaa ccgcgccggt tcctacctgg ggacggcggc gtattcctcg cccgagcagc
     5641 tccggggtga gagggtaacg tccaagagcg acgtctactc gttcggcgcg acgctctacc
     5701 acgctgctgt cgggaagcgt cccttctcgg gtaactccat agaggtcgcc aaccagcacg
     5761 tactcaagat gccgacccct cccagggaga ggggagcccg gataggcgag gatctcgagg
     5821 cccttatcct gggctgcctc gccaagaacc cggccaagcg ccccgacgcc gcccgcctca
     5881 gcgataagct gcaacagagg agcgcggcag acgcgaccgc cgcgttggtc gggtcagccg
     5941 cccgcggtcg cgcaactggt gtctcacgcg tcgcgggggc agcgaagtcg gccgggtctt
     6001 ccggcgtcgg tgccgtcagg caggggttcg ccaggacgag ggcttccggt ccgccagaca
     6061 gcacgatcag cctcccgaca cgcaccttca gggcggggtc gcggcagagg acgatgctgg
     6121 ccatcctgat ggtcgccctc ctcttgctga tcctgaccgt cgcaggagcg tgggcgctgc
     6181 tcggccaggg ggagcagggc aacaataccc cgggtaagga cgtaggaagg caggaacagg
     6241 ccggcgcgca gaagcaggag cccaaggaga atcccaagcc cgacgaagca gcggccccga
     6301 agccctcagg tgagtccagg cccgaagggg gcgctcccga agagaacccc gctccagccc
     6361 ctccgctggc ggaggctgag aaggccgtct acgacctcta ctaccaggag tcgttcgcca
     6421 gagttggcgc gtcgtgggca tttctctccc agaggttaca gaacgagata ggttcgcccg
     6481 agcgatgggc cgagcaggag gacatctaca cgttcaccta catggaattc atctcgtatc
     6541 cggtagccag ggccgtgggg gaagaggccg aggtcacgtt cgaggtgagg ttggatcaca
     6601 cctggggatc agagttgctc tcggggacgt gggtctgcgt caacgagggc ggggagtgga
     6661 agctggaccg cctggagaac gagaggaccg tgcccgcata ggcacggtca agtcttagaa
     6721 atgacattca tcgaccttgc cctataatca cgcgcgtgat caggttcgca gacaggtttg
     6781 tattggagag cgagctgggc tcgggaggca tggcgcgcgt cttcctgggc cgcgacgagg
     6841 tgctcgaccg tgccgtagcc gtgaagttgc tcaaccccgt gcacggcggc acggacatcg
     6901 gcgaccgctt cgagcgtgag ggacgtaccg ccgcccgcct cgcccatccg aacatagtgc
     6961 aggtctacga cgccggtgag gccgaattca acggcaggga gacatccttt atcgtcatgg
     7021 agtacgttcc aggcggcgac ctcaaggcgc tcatagacca gaagggtagg ttgccgggct
     7081 ccgagctcgc caggctcggc gacgaagtct gcgcggggct cgcccacgcc cacgagcacg
     7141 gagtgatcca ccgcgacatc aaaccccaca acatcctcct cgacgagaac gggcacgcta
     7201 aggtaaccga cttcggcata gcccgcgccc tggacgcgac gcaggcgact cgtaccggtt
     7261 acttcctcgg gacggctatg tactcctcgc cggaacagct ccagggacag aaggtgacgc
     7321 cgaagagcga cgtctactcc ctggggacga cgctctacca ggcggcgacg ggagagacac
     7381 ccttcgcagg cgacaccccg atagaggtcg ccagccagca cgtttccaag accccgaccc
     7441 cgccgcgcca acgtggcgcc gacgtgagcg aggatctggc ggccttgatc ctgagctgcc
     7501 tcgccaagaa tccagacgac cgcccgaccg ccgaggaggc acgttcccgg ctacgggagg
     7561 ccattcctac agccggagct acgcaagcgc aaccagcggc ccccacccgg gcggagcaga
     7621 ctcgtcccgc gccgggagac gggcagggtc gcgtttcacg cgaagaccgg cgaggacgga
     7681 ggggaggact cctggccgtc ctggcgcttt ttgccgtgct cgtcgtgata ggagccctcg
     7741 ccgcacagaa cttcatgggt ggaggtcaaa accctggatc gaatggtgcc ggtcagaagg
     7801 tagcgcaggg cgggaatgaa gggcaatccg acggggagcc tggtgggaat gggagccagg
     7861 gtgggggaca gcagagcgcg gcgacttctt ccgcggagtc cgctccctcg caggacaact
     7921 cggggaaggg ggatcaaggc acgctcacgg cgcaggctgc tgaacagacg ataagagatt
     7981 tctacaagac ggccgcgggc tctgactacg agagtgcctg ggacttcctc accccgcggt
     8041 acaagcggga tatagggtcg cgtgggaatt tgaccaacca gtttcggacg ctggaaagca
     8101 tagagtttac ggatgggccg acggccgagg tcaacggaga tactgcggaa gtctcgttca
     8161 gcaccatagc caggcatgaa gatcgcgtcg acaggccgac cctgacggcg acgctcgtca
     8221 aagtggacgg cgagtggaag atagacgatc tataactaga aggcacgggt acggcacgta
     8281 caatggaaga tagcgacgga agggacacac tctgaagacc gtactcacgg cgctcgtctc
     8341 cgcgatcatc ggcggtctcg tcaccgcgct cttcctcctc gtaggggtat acgacctgaa
     8401 ggacgagtcc ggcaacgtga ccataaagga ggtcgcgcca cagaatccgt caggggtgac
     8461 gccggtcgcc gccgacggct cgtcggtgcg cgaagtgtac acgcgggacg gccgtggcgt
     8521 ggtgagcgtg gacgtggccg cgacgtccga aggcgggccg atgggtggct cggggttcgt
     8581 cattgatgag gcgggtcaca tagtcaccaa ccagcacgtc gtcgagggcg ccgaagacat
     8641 ctcggtaagg ttcgcggacg gcgctcgcag agaggccgag atcgttgggc aggacccgtc
     8701 taccgatgtt gcgctcataa aggtagacgc gccgcgggag gcgctgcagc cgctgacgct
     8761 cggggactcg aattccgttg gggtcgggga gccagtcatc gccatcggca atcccttgaa
     8821 cgtcgggatc agcgtcacca cggggatcgt gagcggtctc ggacgcccca taatggcccc
     8881 gaacaactac accataaacg acgccgtgca gaccgatgct gcgataaacc ctggcaactc
     8941 aggcgggccg ctactggatt cgcgcggcac ggtcataggg gtcaacgcgc agatagcctc
     9001 cgagtcaggg ggcttcgaag gcgtgggatt cgccgtcccg atcgataccg tcaagagcgt
     9061 ggtgaagcag ctcataaccg aagggaaggt ggtgcacggc tatatcgggg tatccatgtt
     9121 cgagctgggt atagacgagc tctccgccta caccggcctc tcagaggaat cgctcaggga
     9181 acagtacggc ttccctggca acggggccat agtcagcgag gtgacgcccg acggtccggc
     9241 ggagaaggcc ggcatccgcg gaggggagga gaaggacatc gagggaattg ccgtaccaat
     9301 tggagacgtc ataaccgaga tcgaaggcaa acccatctcc acttcggagg aggtcataga
     9361 ggtcgtaaac tccctcaagc cgggcgagag gctcccgatg accgtagtga cccctggcga
     9421 ggatgaacgt agcgtagagg tcaaggtcgg taccaggccc gagggggttt aggagatgtc
     9481 cggggacgag ttgaagcgcg aggccgcttt cgcggcggtg gagcgttacg tcgagagcgg
     9541 gatggtggtg ggtcttggca ccggatctac ggccagcttc gcggtgcgaa ggatagggga
     9601 actcatggct tctggggagc tgagggacat caagggtgta ccgacctctg cccggaccac
     9661 ggcccttgcc aacgaaatgg gcataccgct ggcgacgctc gcggaggccc gtccgcg
//
LOCUS       DATAIR010000800         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_272620, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000800 DATAIR010000000
VERSION     DATAIR010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272620"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2043)
                     /locus_tag="VHM69_19035"
     CDS             complement(<1..>2043)
                     /locus_tag="VHM69_19035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742542.1"
                     /translation="VARPRLVGRLDPDAGRRLTLVSAPAGFGKSTLLGKWWQGRVDGE
                     RRAAWLSLDGGDNDPGRFLSYLVVAIRKTFREEDEEFGEAVLAALGSSAPPRIDALLG
                     VLVNDVAALPGGLDLVLDDYHTIDSEDVHRIVNFLLDRLPEGVHLVVSGRADPPLHLA
                     KLRARGQMVRLGAADLAFTEEEARAFLRGVMGLDLSAGDVASLKGRTEGWVAGLQLAA
                     LSMQGRGDLHGFVEAFSGTHRDVLDFLAEEVLGRQPDRVRGFMLDTSILSDLTGPLCD
                     AITGRSDGNEMLELLDRENLFVVALDEERRWYRYHHLFAEFLRGRLRRENPELVSELH
                     LRASAWNEENGYTAEAIRHALSAPDHGIAARLIEGGVEGAVERGEGATALRWLEALAT
                     EVKRKRPRLFAQHAVALTITGRPDDVEPLLSEAERAAQADGEDRRFLLGFALAVRAWR
                     ARLRGDASEAVELARGALSLLPDEEASVRNFAAVCLGDGLRTTGDLVAAGEAFAQATE
                     IGRASGHAYASLVGLAMHARVRAEQGRLREADEAFRQSLRLLTEGGFGLLPAAGVVRI
                     GMADLRYERDDLDAAERELEGGVNLAERTGEVSTLVWAYVILSRTRRAQGDDKGALEI
                     ARRAEEAARDSGAELQIAIASSWMARLCLMRGELAEAATLEQERAAASTGAADAARR"
BASE COUNT          315 a          729 c          703 g          296 t
ORIGIN      
        1 cctacgggcg gcatccgcgg cgcctgtcga ggccgcggcg cgctcttgct caagggtggc
       61 ggcttccgcg agttctcccc gcatcaggca cagtcgcgcc atccaggaag aggcgatggc
      121 gatctggagc tcggcgcccg agtcgcgggc ggcttcctcc gcccggcggg cgatctcgag
      181 ggcgcctttg tcgtctcctt gcgcccgcct ggtgcgcgag aggatgacgt aagcccagac
      241 cagggtgctg acctccccgg tgcgctcggc caggttcacg cccccctcta gctcgcgctc
      301 cgccgcgtcc aggtcgtcgc gttcgtagcg cagatccgcc atccctatgc gcacgacccc
      361 cgcggctggc agcagcccga atcccccctc cgtcagcagc cgcagcgact ggcggaacgc
      421 ttcgtccgcc tcgcgcaggc ggccctgctc cgcccgcacg cgtgcgtgca tcgccaagcc
      481 tacgaggctc gcgtaggcgt ggcccgaggc tcggccgatc tcggtggcct gcgcgaaggc
      541 ctcgcccgcc gccacaaggt cgcccgtggt ccttagcccg tcccccaggc aaacggcggc
      601 gaagttgcgg acggacgctt cttcgtcggg caggagcgaa agcgccccgc gggcgagttc
      661 gactgcttcg gaggcgtccc cccgcagtcg ggcgcgccag gcccggactg ccaaggcgaa
      721 acccaggagg aaccggcggt cttccccgtc ggcttgggcc gcccgctcgg cctccgagag
      781 gaggggttcg acgtcgtcgg gtcggccagt tatcgtgagg gccactgcgt gctgggcgaa
      841 cagccgagga cgcttgcgct taacttccgt ggccaaggcc tccagccagc gcagcgcggt
      901 ggcaccctcg ccgcgctcca cggcaccctc gacgccaccc tcgatcaggc gcgcagcaat
      961 cccgtggtcg ggcgcggaaa gcgcgtgcct gatcgcctcg gcggtgtacc cgttctcctc
     1021 gttccaggct gatgcgcgca ggtgcaactc gctgacaagc tcggggttct cacgtctcag
     1081 acggccgcgc aggaactccg cgaagaggtg atggtagcgg taccagcgtc gctcctcgtc
     1141 gagcgccacg acgaagaggt tctctcggtc gagaagctcc agcatctcgt tgccgtcgga
     1201 ccttccggtg atggcgtcgc acaagggtcc ggtgaggtcg gagaggatgg aggtgtccag
     1261 catgaaaccc cgcacgcggt cgggctggcg tccgaggacc tcttctgcca ggaagtccaa
     1321 aacgtcgcgg tgggtgccgg agaaggcctc gacgaagccg tggaggtccc ctcgaccctg
     1381 catggaaagc gccgccagct ggaggccagc gacccagccc tccgtacgtc ccttcaggct
     1441 cgcgacgtcc cccgccgaca gatccaggcc catcacgcct ctcaagaaag cgcgggcttc
     1501 ctcctcggtg aaggcaagat cggcggcccc aagcctgacc atctggccgc gggcgcgcag
     1561 tttggcaaga tgcagcggcg ggtctgcacg gccggagacg acgaggtgga cgccctccgg
     1621 caggcgatcc agcaggaagt tgacgatgcg gtggacatcc tcggaatcga tcgtgtggta
     1681 gtcgtcgagg acgaggtcga gtccgccggg aagcgccgcc acgtcgttga cgagaacgcc
     1741 cagcagggcg tcgatgcgcg gtggcgcaga agagccgagg gcggcgagga ccgcctcccc
     1801 gaactcctcg tcctcctccc ggaaggtctt ccgaatagcg acgacgaggt aagacaagaa
     1861 gcggcccggg tcgttgtcgc ctccgtccaa ggagagccag gcggcgcgac gttcgccatc
     1921 cacgcggccc tgccaccact tgccgagcaa ggtgctcttg ccgaaccccg caggcgccga
     1981 gacgagcgtg agcctgcggc ccgcgtcggg gtccagccgc ccgaccaggc gggggcgcgc
     2041 gac
//
LOCUS       DATAIR010000801         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_169955, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000801 DATAIR010000000
VERSION     DATAIR010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169955"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VHM69_19040"
     CDS             complement(<1..264)
                     /locus_tag="VHM69_19040"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2742543.1"
                     /translation="MTLTLLIGLFTCFVYVESAGAVPNGFGDGKVADVPLPTALDFTP
                     DGRMLVTSKSGQLYVVANGRRSEALNLGPDVCSNSERGLLGVAV"
     gene            complement(464..1534)
                     /locus_tag="VHM69_19045"
     CDS             complement(464..1534)
                     /locus_tag="VHM69_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2742544.1"
                     /translation="MPNFRFDKALKVTRGAKAFLNFGDDVTRGASQDVDEQHENRAAE
                     RKRRRQRKSVLKNELQRKRSELRQITDGIRAAKREARTDEQPEHVKRAKRKQQEIFQI
                     QTELRAVKEGAEGTLVTGALPDFAVIGAAKCGTTFFYHLLTKHPHVEPAATKEIHYFD
                     LLFDEGIEWYRRCFPTPQLKEGRKTITGEASPSYLFELRAPERMAAVAPQARLIALLR
                     NPVDRAYSAYYHRFRSGHRTQTFEETVRVSLERDPRSNHLSQNFYVDYLQRWSSFYSD
                     EQMLVVKSEDFFERPRETMKVVLDFLDLPDWEPGASDLSDRLNKGGYEQEMDPVTRQR
                     LREFFEPHNQRLYDYLGVDFGW"
     gene            complement(1653..2774)
                     /locus_tag="VHM69_19050"
     CDS             complement(1653..2774)
                     /locus_tag="VHM69_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2742545.1"
                     /translation="MMTRKATDMLRRAKSSLDAAARSLRSRAGRRTRRHIAVKDAEIA
                     ALRRELARARGEGGGTVESTPVFFIVGQQKSGTTWLMRMLDSHPEILCKGEGRFFGAG
                     WRQESVKQTDAKRPPSSLYNAVLDAEYLRLWIERSVWSRDDDADEHVDNIVRMAVDYF
                     LMGELSKTGKKMVGDKSPLLTPETIEEISTIYPEARVIHIIRDGRDAAVSAVHHSWNF
                     GKQKSARVSAKREAYRNPGETRESVFAGNSLKRMAAEWGARVGRTIQDGPTLLGSNYA
                     EVRYEDLLQRPEEEIGRLLEFLGAEAGQETVRRCVGAASFERLSKGRKRGEEDATSFF
                     RKGVAGDWKNVFTEEDRRIFKREAGELLIRLSYEKSDDW"
     gene            complement(2781..3626)
                     /gene="rfbD"
                     /locus_tag="VHM69_19055"
     CDS             complement(2781..3626)
                     /gene="rfbD"
                     /locus_tag="VHM69_19055"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX2742546.1"
                     /translation="MKVLLTGSNGQLGRELAGILPERGHGVVALDRKSLDVADPEAVE
                     RVVEEHSPEVVINAAAYTNVDACEKETDLAYSVNALGPRNLAQVCGRSGCELVHVSTN
                     YVFDGERDLPYEPFDPPNPISAYGRTKLAGEEYVKHLTNRWYVVRSAGVYGQGHNFVR
                     TMLRVAGERDSLKVKDDEYISPTYARDLATGIVRIIESGHYGLYHLTNSGSCSWYEFT
                     REIFRLAGVETEVVPIPGSDYPLPAARPANGILSSLGSPELRHWSEALVDFLEGEGVL
                     DGARG"
     gene            complement(3642..>4092)
                     /locus_tag="VHM69_19060"
     CDS             complement(3642..>4092)
                     /locus_tag="VHM69_19060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2742547.1"
                     /translation="LSVHGSEEGYESYADELRDLAGDDGRISFRGAFARGELRSVLAG
                     IDVLVVPSRWYENAPGVIFEAFVAGTPVIATDLGGMSEFVRHGRNGLLFRLEDAQDLA
                     RQLRRLAEKPGLLPELRAGIGAVKSVGEYADEMEQLYDTLLNRRAKY"
BASE COUNT          725 a         1370 c         1138 g          859 t
ORIGIN      
        1 cacagccacc ccgagcagcc cgcgctcgga gttggagcac acgtcagggc cgaggttgag
       61 agcctcagac ctccgcccgt tagccaccac gtagagctgt ccagacttgc tggttaccag
      121 catccggcca tcaggcgtga agtccagagc cgtcggcaag ggcacgtcgg ccaccttccc
      181 atcgccgaat ccgttcggta cggcccccgc agactctaca tagacaaagc acgtaaagag
      241 gcctatcaac agggtcaggg tcaaggacac caacacacct ctagacaaaa cggggcctct
      301 cttcacgtcg tcgaactctc caccgaaggc cgatagcgtc acaggctaat acttgacggc
      361 ttagtactat tgtacaataa ggattaatac caaggcataa aaagcttagt accaacatac
      421 accccttgca ggacgcccca ggcttgcggg ttatctggac cctctaccag ccgaagtcca
      481 cgccgaggta atcgtagagc ctctggttgt gcggctcgaa aaattctcgt aggcgctgcc
      541 tggtgacagg gtccatctcc tgctcgtatc cgcccttgtt gagcctgtcg ctcaggtccg
      601 aagctccagg ttcccaatca ggcaggtcga ggaaatccaa gacgaccttc atggtctccc
      661 gcgggcgctc gaagaaatcc tcgctcttta cgaccagcat ctgctcgtcg ctgtagaagc
      721 tcgaccagcg ctgaagatag tccacgtaga agttctggga caggtggttg gagcgtggat
      781 ctctttcgag ggagactcgt acggtctctt cgaacgtctg ggtccgatgc ccgcttctga
      841 accggtggta ataggcggaa taggccctgt ctacagggtt ccgcaataga gctatcagcc
      901 gtgcttgcgg cgccaccgcg gccattctct ccggagcgcg aagttcaaac aagtacgatg
      961 ggctcgcctc tccggtgatg gtcttcctgc cctccttcaa ttggggcgtc gggaaacacc
     1021 ggcggtacca ctcgatgccc tcgtcgaaga gcaggtcgaa gtagtgtatc tccttggtcg
     1081 cggcaggctc gacgtgcggg tgcttggtca gaagatggta gaaaaaggtc gtcccacact
     1141 tcgcggcccc gatcacggca aagtcgggca gcgcgccagt caccagcgta ccctcagctc
     1201 cctccttcac ggcgcgaagc tcggtctgta tctggaaaat ctcctgttgc ttccttttgg
     1261 ccctctttac gtgctcgggc tgctcgtctg tgcgcgcttc gcgcttcgcg gcacgaattc
     1321 catctgttat ctgacgcagt tcgcttctct tacgttgtaa ctcgttcttg agcacggact
     1381 tgcgttgccg gcgcctcttc cgctccgcag cccggttctc gtgctgctcg tcgacgtcct
     1441 gtgaagcgcc gcgcgtgacg tcgtctccga agttgagaaa cgccttcgcc cctcgcgtaa
     1501 ctttcaacgc tttgtcgaat cggaagtttg gcaagcaatg cctccttctt cgcctcgtct
     1561 aatccgcggc ctgcatgacg aaggtttcaa cgccagcgtc cccatcagcc cgaagtctaa
     1621 aggctcttcg ttagatctgc tcgccatgta gcctaccagt cgtcactctt ctcgtaggac
     1681 agcctgatca ggagttctcc agcttctcgc ttgaagatgc gcctgtcctc ctcggtgaag
     1741 acgttcttcc agtcgccggc cacccccttc ctgaagaaag acgtggcgtc ctcctctccc
     1801 ctcttcctcc ccttcgagag cctctcgaag ctggctgcgc caacgcacct tctcactgtc
     1861 tcctggcccg cctcggctcc gaggaactcg agcagacgcc ctatctcctc ttccggcctc
     1921 tgcaggaggt cctcgtagcg tacttcggcg tagttgctcc cgagcagcgt ggggccgtcc
     1981 tgaatggtcc taccaaccct ggctccccac tccgcggcca tcctcttgag cgagtttccg
     2041 gcgaatacgc tctcgcgcgt ctcgccggga ttcctgtaag cctcgcgctt cgccgagacc
     2101 ctggcgctct tctgctttcc gaagttccag gaatggtgca ccgcagagac cgccgcatcc
     2161 cgtccgtccc tgataatgtg gatgactctc gcctcggggt agatggtgga gatctcctct
     2221 atcgtctcag gggtgaggag cggagatttg tcccccacca tcttcttgcc ggtcttggag
     2281 agctcaccca tgaggaaata gtcgacggcc atgcggacga tgttgtccac atgctcatca
     2341 gcatcatcgt ccctgctcca gacggacctc tcgatccaga gcctcaggta ctccgcgtcg
     2401 agcacggcgt tgtagagtga gctgggcggc cgcttcgcgt cagtctgctt tactgactcc
     2461 tgccgccagc ccgcgccgaa gaagcggccc tctcccttgc acagtatctc cgggtgggag
     2521 tcgagcatgc gcatcaacca cgtggtgccg gatttctgct ggcccacgat gaagaagacg
     2581 ggcgtactct cgaccgttcc tcctccttca cccctcgctc gggccagctc cctcctgagc
     2641 gcggcgatct ccgcgtcctt gacggcgata tgcctgcggg tccggcgtcc cgccctcgac
     2701 ctcagagatc tcgccgcggc gtcgagagac gatttcgccc tccgaagcat atctgtggcc
     2761 ttgcgggtca tcatcggccc tcagccacgg gcgccatcga gcacgccttc accctccagg
     2821 aagtccacaa gcgcctcgct ccaatgccgg agctcgggac tccccaggga ggaaaggatc
     2881 ccgttcgccg gtcgcgccgc cggcagaggg taatccgagc cggggatggg gacgacctcg
     2941 gtctcgaccc cggcgagacg gaagatctct cgggtgaact cgtaccagga gcacgaccct
     3001 gagttggtga ggtggtagag gccgtaatga ccggactcta ttatcctcac gatgcccgtc
     3061 gccagatctc tggcgtacgt cggcgagatg tactcgtcgt ccttgacctt caaggagtcg
     3121 cgctcgccgg ccacgcgcaa catcgtcctc acgaagttgt gtccctgccc gtagacgccg
     3181 gcggagcgta cgacgtacca gcggttcgtg agatgcttca cgtactcctc gccggccaat
     3241 tttgtacgcc cgtaggcgct tatgggattc ggggggtcga agggttcgta gggaagatcc
     3301 ctctccccgt cgaagacgta gttggtgctc acgtgcacaa gctcgcagcc gcttcgcccg
     3361 cagacctggg ccaggttgcg cggtccaaga gcgttcaccg agtacgcgag atccgtctcc
     3421 ttctcgcagg cgtccacgtt agtgtaggct gcggcgttga tcactacctc cggagagtgc
     3481 tcctcaacga cccgctctac ggcctcaggg tcggccacgt cgaggctctt cctgtctagc
     3541 gccacgaccc catggccccg ctccggaagt atgccggcca gttcccgtcc gagctggccg
     3601 ttagagccgg tcagtagtac cttcatcctg acaagttctc cttaatactt tgcccgccta
     3661 ttcaggagcg tatcatagag ttgttccatc tcgtcagcgt actcgccgac gctctttacc
     3721 gcgcctatcc ccgcgcgcag ttccgggagc aggccaggct tctctgccag ccgccgcagt
     3781 tgcctggcga ggtcctgcgc gtcctcaagc ctgaagagca gaccgttacg tccatgtcta
     3841 acgaactcgg acatacctcc gaggtcagtt gcgatcaccg gcgtccctgc gacgaaggcc
     3901 tcgaagatca cgccgggggc gttctcgtac cagcgggacg gcaccacgag gacgtcgatc
     3961 cccgccagga cgctcctcag ctccccgcgc gcgaaagcgc cgcggaagga gatccgcccg
     4021 tcgtccccgg cgagatcacg cagttcatcc gcataggact cgtagccctc ctcgctcccg
     4081 tggacggaga gc
//
LOCUS       DATAIR010000802         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_366647, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000802 DATAIR010000000
VERSION     DATAIR010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366647"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VHM69_19065"
     CDS             <1..1301
                     /locus_tag="VHM69_19065"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2742548.1"
                     /translation="DRYVEGTCPVCGYKEARGDQCDNCGSWYEAYELIDPHSKVTGGP
                     VEVRETTHLYLDLPKFTDRLREYVAGQNHWRTSVKNFILGIIDGGLEKRPITRDIQWG
                     VPVPEPGFEDKRFYVWFDACIGYVSSTMEWAENVGEPDRWREYWQEPETKLIHFIGKD
                     NTVFHTLIWPAQLMGTAEDGDEPFILPYDVPANEFMNLEVVIDGERRAVQMSTSRNLA
                     VWLHEALDRFPADPLRFYLASNLPETGDVVFSWREFQSRVNSDLIGNLGNYVNRVLSF
                     TERYFDGELPRPDKLPEDILAVLEDFRGLESRYEARMLALRPREALGELLAMGRRANQ
                     FFDASAPWKTRRDDPEQTRVTLYVCSVLLGSLAYHASPFVPDAVERLKGFFDGPISRV
                     SDFEDLPKDYRVKGARPLFRRIEDDEVEAAEEQLARAVRG"
     gene            complement(1298..1657)
                     /locus_tag="VHM69_19070"
     CDS             complement(1298..1657)
                     /locus_tag="VHM69_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2742549.1"
                     /translation="MEKSYDELVAEAKAETEQADVEAVHEALSSGEHITIVDVREPDE
                     YAEEHILQAKHIPRGMLETQAAEQLPVKEARIVTHCGAGGRGSLAAKSLEEMGYTNVA
                     NLEGGLNAWRARGYETA"
     gene            complement(1659..>1896)
                     /locus_tag="VHM69_19075"
     CDS             complement(1659..>1896)
                     /locus_tag="VHM69_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2742550.1"
                     /translation="RSLLPNETEAPMVFTGNVRALRHIIEMRADAHAESEIRNLALRL
                     FLCLRTVDPILFGDYELGELPDGTFTVSTKYRKA"
BASE COUNT          344 a          612 c          577 g          363 t
ORIGIN      
        1 ccgaccgtta tgtcgaggga acgtgccccg tgtgcggtta caaggaggcc aggggcgacc
       61 agtgcgacaa ctgcggctcg tggtacgagg cgtacgagct catcgacccg cactccaagg
      121 taacgggcgg acccgtggag gtgagggaga cgacgcacct ctacctcgac cttccgaagt
      181 tcaccgaccg cctcagggag tacgtcgcgg gacagaacca ctggcgcacg tcggtcaaga
      241 acttcatcct cggcatcata gacggaggtc tcgagaagcg gccaataacc cgggacatac
      301 agtggggcgt ccccgtcccc gagcccggct tcgaggacaa gcgcttctac gtgtggttcg
      361 acgcctgcat cggctacgtc tcatcgacga tggagtgggc cgagaacgtc ggagagcccg
      421 acaggtggag ggagtactgg caggagcctg agaccaagct catccacttt atcgggaagg
      481 acaatacggt ttttcacacc ctgatctggc ccgcccagct catgggcacg gccgaagatg
      541 gagatgaacc cttcatactc ccctacgacg taccggccaa tgagttcatg aacctcgagg
      601 tcgttatcga tggagagcgg cgggcggtgc agatgtcgac gagccgcaac ctcgctgtat
      661 ggttgcacga ggcccttgac cgcttcccag ctgaccctct gcgcttctac ctggcctcga
      721 acctgccgga gacaggtgac gtggtgttct cctggcgcga gttccagagc agggtgaact
      781 ctgacctgat cggcaacctc ggcaactatg taaaccgcgt tctctccttt acggaaagat
      841 atttcgacgg cgaactacca cgccccgata aactacctga agacatcctc gcggtccttg
      901 aggacttcag ggggctcgag tcgcgctacg aggccaggat gctggccctg aggccgcgcg
      961 aagcgctggg ggagctcctg gcgatgggca ggcgcgccaa ccagttcttc gacgcgtcgg
     1021 ccccgtggaa gactcgcaga gatgatcctg agcagacccg cgtcaccttg tacgtctgct
     1081 ccgtcctgct cggctccctc gcgtaccacg cctccccctt cgtccccgac gccgtcgagc
     1141 ggctgaaggg attcttcgac ggtcccatct cccgcgtctc ggacttcgag gacttgccga
     1201 aggactaccg cgtcaaagga gccaggccct tgttccgcag gatcgaggac gatgaggtcg
     1261 aggcggccga ggaacaactc gccagggccg tccgcggcta ggcggtctcg tagcctctcg
     1321 ccctccaggc gttcaagccg ccttccaggt tggccacgtt cgtgtagccc atctcctcga
     1381 ggctcttggc ggcaagagag ccccggcctc cggcaccgca gtgggtgacg atgcgtgctt
     1441 ccttgacggg tagctgctcg gccgcctgag tctcgagcat cccgcgtggt atgtgcttcg
     1501 cctgcagtat gtgctcctct gcgtactcgt cgggctctcg cacgtccacg atggtgatgt
     1561 gctccccgga actcaacgct tcgtgtaccg cttctacatc cgcctgctcc gtctcggcct
     1621 tcgcctcggc caccaattca tcgtaactct tctccatcct aggccttgcg gtatttggta
     1681 gataccgtaa aggtgccgtc ggggagctct cccagctcgt aatctccgaa gaggatgggg
     1741 tcaacggtcc gcaaacacag gaagaggcgc agggcgaggt tgcggatctc gctctccgcg
     1801 tgcgcgtctg cccgcatctc gatgatgtgc cttagagcgc gcacgttccc cgtgaagacc
     1861 atcggggcct cggtctcgtt cggcaacaag gaccgc
//
LOCUS       DATAIR010000803         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_273084, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000803 DATAIR010000000
VERSION     DATAIR010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273084"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VHM69_19080"
     CDS             <1..458
                     /locus_tag="VHM69_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX2742551.1"
                     /translation="DLALLVAEGRREEFALFPEFSDPATRESIPDPGAEETFRTAVLD
                     WASLGEPEHAAHLELYRGLIALRREMIVPRLSGIPGGEAEYEIIDERALSVRWTLGDG
                     SLLALLANLGPEPLEVPGWPPGESLYASEGVSEDGRDLPGWSVSWHLAG"
     gene            428..>2987
                     /gene="treY"
                     /locus_tag="VHM69_19085"
     CDS             428..>2987
                     /gene="treY"
                     /locus_tag="VHM69_19085"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX2742552.1"
                     /translation="MVGVVAPGRMRIPRATYRLQLTEDFTFSDAADLVPYLAELGVSD
                     LYASPYLKARPGSMHGYDVVDPTSLNPELGGEKEHGRLVEALEEHGMGQLLDIVPNHM
                     GVASDNAWWSDVLEHGPASEYAPFFDIDWCPSNRKELWGRVLLPVLGDHYRAALERGE
                     IRLSFEEGTFWVNYYEHRYPIDPQTYPLILNVISLPGEDEYGSEIERLVTAFGDLPGR
                     DATDERNVAGRARNAANLKADLAALCSTSSGVMDAVEERIHRLNEGLGNPRFEGLHLL
                     LEAQAYRLAYWRVGSDEINYRRFFAVDDLAGVRVEDPLVFEATHRLILQLVEKGVVNG
                     LRIDHPDGLHDPAGYLSRLKDAVSEASGGPVYTLVEKILAHNERLPEDWPVSGTTGYE
                     FTNLLNGLFVDPAGEGGTDEAYQSFIGGPIDFQSLLYECKHKVMRTMLSSELNALSHC
                     LKRISEHERSHDFSIETLREALSELVAHFPVYRTYIRSGHISDADRRHLDLAVQEAKE
                     KSTGGDPRVFDFISYVVLRHGSEEIGADAFTSKFQQYTGPVMAKGMEDAALYIYNRLI
                     SLNEVGGEPEIFGVSVPEFHRLNVESIERWPHSMLSTSTHDTKRSEDVRARINVLSEI
                     PGEWNERLGRWSKLNDPFRREVDGSTAPSRNDEYLLYQTLLGAWPPGEPDGEELEAFR
                     KRVKAYMEKAMREAQVRTSWTDANEEYEDAVTGFVDALLSKSNSFLQEFLPFQRRVAR
                     IGALNSLSQSLIKLTAPGVPDVYQGNEIWDFSLVDPDNRRPVDFELRKRLLAELRELD
                     PSDARTLLEDSAWQDGRPKLYIIWKALAMRRESQELFRDGDYVALQTSGEGSENL"
BASE COUNT          602 a          905 c          934 g          546 t
ORIGIN      
        1 ctgacctcgc gcttctcgtg gccgagggaa ggcgcgagga gttcgccctg tttcccgagt
       61 tctccgaccc cgccacgcga gagagcatcc cagacccggg ggcggaggag acgttccgca
      121 cggccgttct ggactgggcg agcctgggcg agccggagca tgccgcgcac ctggagttgt
      181 accggggatt gatcgcgctc aggcgagaga tgatcgtgcc tcgtctgtcc ggcatcccag
      241 gcggtgaagc tgagtacgag ataatagacg agcgggcact gagcgtgcgc tggacgctgg
      301 gtgacggctc ccttctggcg ctgcttgcga acctgggacc ggagccgctg gaagttcccg
      361 gatggcctcc tggcgaaagc ctgtacgcca gcgagggtgt gtcagaggat ggacgtgacc
      421 ttccgggatg gtcggtgtcg tggcacctgg caggatgagg ataccccgcg ccacctatag
      481 gctgcagctc actgaggact tcactttctc tgatgccgca gatctggtgc cttacctggc
      541 ggagctgggg gtcagcgacc tctatgcctc gccgtatctg aaagctcgcc cgggcagtat
      601 gcacggttac gacgtcgtgg atcctacctc cctgaaccct gaactcgggg gtgagaagga
      661 gcacggacga cttgtagagg cgctcgaaga acacggtatg ggccagctcc tagacatcgt
      721 acccaatcac atgggcgtgg cgagcgacaa cgcctggtgg tccgacgtgc tggagcatgg
      781 tcccgcctcg gagtacgcgc ctttcttcga tatagactgg tgtccttcaa acagaaaaga
      841 gctatggggc agggtcctgc tcccggtcct tggcgaccac taccgcgccg ccctcgaacg
      901 cggtgagatc cggctgtcct tcgaagaagg aaccttctgg gtcaattact acgaacaccg
      961 ttacccgatc gacccgcaga cttacccgtt gatcctcaac gtcatatctt tgcccgggga
     1021 agacgagtat ggctctgaaa tcgagcgtct cgttaccgcc ttcggcgacc ttcccggacg
     1081 ggatgccaca gatgaaagaa atgtagccgg gcgagcgcgg aacgccgcaa acctgaaagc
     1141 ggacctcgcc gccctgtgct caacatcttc cggagtgatg gacgccgtcg aggagcgcat
     1201 ccacaggttg aacgaggggc ttggaaaccc gaggttcgag ggtttgcacc ttctcctcga
     1261 agcacaggcg tacaggctcg cctactggcg ggtgggaagt gatgagatca actataggcg
     1321 tttcttcgcc gtcgatgacc tggccggtgt tcgtgtcgag gacccgctcg tcttcgaggc
     1381 gacgcacagg ctcatactgc aactcgtaga gaagggcgtt gtaaacggtt tgcgcataga
     1441 ccaccctgac gggctgcacg atcccgccgg ttacctcagt cgtctgaaag acgcggtatc
     1501 ggaggcgtcg ggagggcctg tctacacgct cgtcgagaag atcctggccc acaacgagcg
     1561 actgcccgag gactggcctg tctcagggac cacgggctac gagttcacga accttctcaa
     1621 cgggctcttc gtggacccgg ccggcgaagg cgggactgat gaggcgtacc agagcttcat
     1681 cgggggcccc atcgacttcc agagcctgct ctacgaatgc aagcacaagg ttatgcgcac
     1741 aatgctttcg agcgagctga acgcgctctc gcactgtctg aagcgcatct cggagcacga
     1801 acgctcgcac gacttcagca tcgagactct gcgcgaggcg ctctccgaat tggtcgcgca
     1861 tttccccgtc taccgcacct acattcgtag tgggcacatc tctgatgcag acaggcggca
     1921 cctggatctc gccgtgcaag aggcgaagga gaaaagcacg ggcggcgacc cgagggtctt
     1981 cgatttcatc agctacgttg tccttcgcca tgggtctgaa gagatcggag cggacgcctt
     2041 tacttcaaag tttcagcaat acacgggacc tgtgatggcg aagggcatgg aggatgcggc
     2101 cctgtacatc tacaaccgtc tgatctccct gaacgaggtc ggcggcgagc cggagatctt
     2161 cggtgtctcc gtccccgagt ttcaccgcct gaacgtcgag agcatcgagc gctggccgca
     2221 ctcgatgctc tcgacctcga cgcacgacac caagcgcagc gaggacgtgc gggccaggat
     2281 caatgtcctc tcggaaattc ccggcgagtg gaacgagaga ctcggccgat ggagcaagct
     2341 caacgacccg ttccgccgcg aggttgacgg gtctacggcc ccgagccgca acgacgagta
     2401 cctcctttac cagacgctac tcggtgcctg gccaccagga gaacccgacg gagaggagct
     2461 ggaagcgttt cggaagcgcg taaaggcgta catggagaag gcgatgcggg aggcgcaggt
     2521 gcgcacgtcg tggaccgacg cgaacgagga gtacgaggat gcggtcaccg gtttcgttga
     2581 cgccctactc tctaagtcca actccttctt gcaagaattc cttcccttcc agcgtcgcgt
     2641 ggcccgcatc ggcgccttga acagcctctc ccagagcctg atcaaactta cagcaccagg
     2701 cgtccccgac gtgtatcagg gcaatgagat ctgggacttc agcctcgttg acccggacaa
     2761 ccgccgtccg gtggacttcg agctgcggaa gagactcctc gctgaactga gagaactcga
     2821 cccctccgac gcacgcacgc ttctcgaaga cagcgcgtgg caggacggtc gtccgaagct
     2881 ctacatcatc tggaaggcgc tggcgatgcg ccgcgaaagc caggaactct tcagagacgg
     2941 cgactacgtg gcgcttcaga cttcgggcga aggcagcgaa aacctcg
//
LOCUS       DATAIR010000804         8947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_229530, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000804 DATAIR010000000
VERSION     DATAIR010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229530"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VHM69_19090"
     CDS             complement(<1..968)
                     /locus_tag="VHM69_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2742553.1"
                     /translation="MTLIGLRSIDKFYGGRAVLRSLEMNVNAGVRIGLVGANGTGKST
                     LLRLLAGTEEVDGGEVIRRRGLSVASLPQYVEGDMRTPMEVVLAARPEVSELQKELEA
                     CEEQLGSPGVAADLRRMQRVLERQERLLRRFTELGGPGFEGEARGYLRSLGLGDGDVE
                     RPMSDLSGGQRKLAVLASCLARRPDVLLLDEPEAHLDAERRERLEAIVRAFDGAVVIT
                     SHDRYLLDETVEQIAELEDGRITSWPGNYSAYTVARELKIKRQQQLYVSQQKEIARLE
                     EAIKRFKLWASMVENERHIKQARNKQRQIDRMDKVERPVLERRKIGL"
     gene            complement(1067..1855)
                     /locus_tag="VHM69_19095"
     CDS             complement(1067..1855)
                     /locus_tag="VHM69_19095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2742554.1"
                     /translation="MTERLYKEDPLTPEEREELEADLIAFCDRELGLLGDIGGLDVLY
                     AGGSSPLWIEGLSQRVGEDGSVTAIDLDVERVEEARVSLGEAELSAPVHPLAGDIFEI
                     PFGPGTFDLVYSSGLFHELDVSEKSGQEALAALRDVARPGGRVATSDFVGSEPAAQLE
                     DEELGADLMREVFRKELYGIGPPDRLVALHEELLADVRWRVSPPWKIRHLEKLVLSED
                     EPAELVSLPTATATRFRERREALRTRILREGYRRPATIYVEGVV"
     gene            2567..3532
                     /locus_tag="VHM69_19100"
     CDS             2567..3532
                     /locus_tag="VHM69_19100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742555.1"
                     /translation="MVVVLLVLAVLGAIYQTIATKLAERAYPPPGEMVDVKGYSLHIN
                     CVGQGSPTVLLDSGLGLFSAQWVRVQREVSGTTRVCAYDRAGMGWSEMGPDPRDAKQI
                     TGELHTLLSKASIDGPYVLVGHSLGGLSMLTYANRYPDEVAGVTLVDSSTELDQFSQR
                     PEARDSNEPQEQTSPVVSQLVRLGVSLPARLGIVRLLSKFDPASPELPPQQRAQSDAL
                     TPSTRQFTTSVLEFLAPTQTRRLGSLGNKPLAVVTAGTQEPSWLKRQDELATLTSSNS
                     IHRVVKGATHESLLYDRSDAQATSAAIVEVVAAVRNEQPLAQENP"
     gene            3649..3864
                     /locus_tag="VHM69_19105"
     CDS             3649..3864
                     /locus_tag="VHM69_19105"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX2742556.1"
                     /translation="MNPPHLSDFGNLDTATLMRHLPGLKFPAEKEQVVSTAENNRVPQ
                     EAVQRIRHTSRQRFNSPEEVLRAVQGH"
     gene            3879..4331
                     /locus_tag="VHM69_19110"
     CDS             3879..4331
                     /locus_tag="VHM69_19110"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX2742557.1"
                     /translation="MGNERLINYLRFAEHGAELVSSESTGAFVLAAAGLLEGRKATTH
                     PAFAELLKKLGVNYVQSYSVEDGRFLTMQGVSGGIDSMLKLVAKLKSEAAARRAQYII
                     EYDPQPPFGGVDWSEANGDGLAQMLEEHRADFGRELAGRPDLFQKLFI"
     gene            4394..5101
                     /locus_tag="VHM69_19115"
     CDS             4394..5101
                     /locus_tag="VHM69_19115"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX2742558.1"
                     /translation="MTAEEQNQKTIAFVLYPGLTLFDLAGPLQVFAGVSQLVPQYRTV
                     VVGENAEPADTDLHVKLIPTHTFEEVPHPSVLLVPGNRTSTFKAMSNEALRSYVRSAA
                     ATADVAGSICTGALILASVGLLEGRQATTHWVYYKIMEEFGATYVRKRWVEDGKFISS
                     AGVSAGIDMALHLAARLTDEETARRVQRSLGYDPHPPFGGMDYGHLGALSAIRAGLSM
                     AAPIIAARPKRLTKQNR"
     gene            5157..6755
                     /locus_tag="VHM69_19120"
     CDS             5157..6755
                     /locus_tag="VHM69_19120"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2742559.1"
                     /translation="MEISSEAYATLNPTELDNFKNPLRLPGESGAMGVLDASDLPTRI
                     TAQKERVEIVPGKSSELWAYRAERDGKTYVNPTFRVQKGKEFSAEFANDLDEETTVHW
                     HGLHVDWRMDGHPFRPVAPGSTYRYVFPLQDRGGTYWYHPHPHGGTARQTYAGLAGFF
                     LIEDEEQQRLDEALDLSLGETDIPLLIQDKILGESGNFVYEPDAMAVDMGYEGDVILV
                     NLTPTPYLEVSSRIYRFRLLNGSNARTYRVAFTKPGDEELLPYQVIGTDGGLLDRPRA
                     TREVFLSPGERIDVLLDLRNFEVSEELVLKSLSFDPMHREHEMGGSMDHMEHMDMGHH
                     MDMGHHHMHHQMGPANLPDGSEFYLLKLVIAEKTGYSRSVPEVLSEMPQPDLGGADTR
                     PITISMTTDDEGMRWLINGETHTMDEFPVVVRRGAKEVWEIHNDEKSMPHPAHLHGFQ
                     FRILERAGTPEQVAHLVVEEKGRLATDVGFKDTVLLWPGETVKCVMDFSHGFEGEQIY
                     MFHCHILEHETTMMLNVKIADSQG"
     gene            complement(6812..>8947)
                     /locus_tag="VHM69_19125"
     CDS             complement(6812..>8947)
                     /locus_tag="VHM69_19125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX2742560.1"
                     /translation="HGVDAQPISMGTKHDRMQYEMPATPADHGDHMDHDMSDPAMAKA
                     MERHMRNRFFVALILTIPTVLYSPLGINLFGLRLPTFGLGENAIMLLLSTPVVFYCGW
                     IFISGSYHSLRRRTLNMSVLIATGVLAAYIFSVLITFLGGETFYEAAAMLVTFVLFGH
                     WMEMRSRRGTSDALRSLFDLVPPQATVMRDGEEVEIPSSEVRLNDIVILRPGDKVPVD
                     GEVTEGETSIDEALVTGESVPVTKRAGDAVIAGSINRSGSVRFRATAVGAETTLAQIV
                     DLVQKAQNSKAPGQRLADRAAEYLVILAVGSGVVTFLAWYFLGGAAAITAMTFAISAV
                     VIACPDALGLATPTAVAVGTGIGARHNILIKDAATLENVSRIDAVVLDKTGTLTEGEP
                     SLTDVSTLNGRSEDDLLRLVAPAEKGSEHPLAEAIVAGARERGIEVGDAAHFDSIAGH
                     GIRAEVNGQTVLVGNLKLMDESGVDVFKLEDRATALAREGKTPMFVAVDGEPSGLVAV
                     ADTIKPSARDTLRRLKEMGIEAVMITGDNKRTAEAVARELGIERVFAEVLPADKARYV
                     QQLQREGKRVAMVGDGVNDAPALAQADIGIAIGAGTDVAIETAKVVLMKSDPLDVTRA
                     VVLSKATVRKMKQNLVWASVYNVLAIPVAAGVLYPAYGIMLRPEWSALLMSVSSIIVA
                     VNAVLLKGVEGDLDEPSTGGRSSEPSPAA"
BASE COUNT         1807 a         2757 c         2708 g         1675 t
ORIGIN      
        1 agccctatct tgcgccgctc cagcaccggg cgctcgactt tgtccatgcg gtcgatctgg
       61 cgctgcttgt tgcgggcctg cttgatgtga cgctcgttct caaccatgct ggcccagagc
      121 ttgaagcgtt tgatcgcctc ctccagccgc gctatctctt tctgttgaga gacgtagagt
      181 tgctgctgcc gtttgatctt tagctcgcgc gcaacggtat atgcggagta gttgccgggc
      241 cacgacgtga tcctcccgtc ttccagctcg gcgatctgct cgaccgtctc gtccagcagg
      301 tagcggtcgt gtgaggtgat gacgacggcg ccatcgaaag cgcgcacgat ggcctcgagc
      361 cgctccctgc gctcggcgtc gaggtgggct tcgggctcgt cgagtagcag cacgtcggga
      421 cgccgagcta ggcacgaggc gaggacagcc agtttgcgct gcccgccgct caggtcactc
      481 atcgggcgct cgacatcgcc atcgccgaga cccaaagacc tcaggtagcc gcgcgcctca
      541 ccctcgaatc cgggcccacc gagctccgtg aagcggcgca agagccgttc ctgacgttcg
      601 aggacccttt gcatcctgcg caggtcggcg gccaccccgg gcgagccgag ttgttcctcg
      661 cacgcttcca gttccttctg aagttccgag acctctggcc gcgcggccag cacgacctcc
      721 atcggcgtgc gcatgtctcc ttcgacatac tggggcaaag aggcgacgct caggccgcgg
      781 cgccggatca cctccccccc atcgacttct tccgtgcctg cgaggaggcg aaggagtgtg
      841 gacttacctg ttccgttggc gccgacgagc ccgatcctca cccccgcgtt cacgttcatc
      901 tccaggctac gcagcacggc ccgtccgccg tagaatttat cgatggacct cagtccgatg
      961 agggtcatgc ggccctccca tctcaggcta aacgtgtttc tctaaccggg cgaatgatga
     1021 cgcccgccgg gtaccggcgc atcacgcaat tggatgatct tcgagattaa accacgccct
     1081 cgacgtagat ggtggcggga cgcctgtagc cctcgcgcag gatacgggta cgcagcgcct
     1141 cgcgccgttc ccgaaaacgc gtcgctgtcg ccgtcggcaa cgaaaccagc tccgccggct
     1201 catcctcaga caacacgagc ttttcgaggt ggcggatctt ccacggaggc gagactcgcc
     1261 atctcacgtc ggcgagaagc tcctcatgca aggcgacgag cctgtcgggt ggcccgatcc
     1321 cgtacagttc ttttctaaac acctcacgca tcagatccgc tcccagctcc tcgtcttcta
     1381 gctgggccgc cggttctgag ccgacgaagt cgctggtcgc gaccctgccg cctggccttg
     1441 ctacatcgcg gagcgcggcg agtgcctcct gcccgctctt ctccgatacg tccagctcgt
     1501 ggaacaaacc tgaggagtag acgaggtcga aggtgccagg gccgaaaggt atctcgaaga
     1561 tgtctccggc cagagggtgg accggagcgg aaagctccgc ctctcccaga gagactcgtg
     1621 cctcttcaac ccgttccacg tccaggtcga tcgcggtgac gctgccgtcc tccccgacac
     1681 gctggctgag gccctcgatc cataatggtg acgagcctcc cgcgtaaagg acatcgagtc
     1741 cacctatatc cccgagaagg ccgagctcac ggtcgcagaa agcgatgagg tcggcttcga
     1801 gttcctcccg ctcctccggc gtcagggggt cctctttgta taatctctcc gtcatcagat
     1861 tcccgaactg caaatatgtc cttcaggttc agggtatcca cgcctcccgg cgacaaccta
     1921 gtcgggcccg gtgaggagcg agtagaactg accagagtat agataatgga agcgtactgc
     1981 gcgcggctcc tctagcatca agcaaccgta gtaactaccg gcgaacgcga gggcaggagc
     2041 accttacggt caaggtactg gctcaaaccg gctcatataa acatccatgt caggcaggcc
     2101 gtacctgtgg tcatggactc ggggctgtaa ctcctgctac ccctccccgc cccgtatctg
     2161 cgtcggtgtt ctggaagaat atcgcgcatc cacccgagcg catctttatg cgcacgtgca
     2221 acatgaagtg gtcgactcct taaggagccg agaagtctca gcgcgacgtt aggatggcca
     2281 gcgagcgggg ctatgcagca tcctgcaaac gaacttcgta caaccttcct ttgaaactgt
     2341 ctgataaatc aggattcgcc acaaactggg tgccgcgggc ctgtaaaacg ggacagagaa
     2401 gctgtatatg accttgttgg gtcccgagac gcaagatcga gactcccttc ggggatttat
     2461 ctgacttcgg cacaagcaca agacctcgtt agggattaat cttagggtaa tgagcagcac
     2521 tacggccaga aggaccatgc ggcggatctg gaggccgctc ctggggttgg tcgtcgtcct
     2581 gttggtcctc gctgtcctcg gagcgatcta ccaaaccatc gctacaaagc ttgccgagcg
     2641 cgcctatccc ccgcctggtg agatggtcga cgtaaagggc tacagcttgc acattaactg
     2701 cgttggccag ggcagcccga ccgtgctctt ggattcggga ttggggcttt tttcggccca
     2761 atgggttagg gtgcagcggg aggtctccgg taccacgcgt gtgtgcgcct acgaccgcgc
     2821 ggggatgggt tggagcgaga tgggtcccga tccccgggat gccaagcaga tcacaggcga
     2881 acttcacacc ctgctaagca aggctagcat cgatggccct tacgtgctgg tgggccattc
     2941 gttaggtgga ctgtccatgc taacgtacgc caaccggtat cccgatgagg tggcgggcgt
     3001 gaccctggtc gattcttcta ctgagctgga tcagttcagc cagcgaccgg aagcacggga
     3061 tagcaacgaa ccacaagagc agacatcccc cgtggtttcc cagctggtgc ggctcggggt
     3121 ttccctgccg gcgaggctcg ggattgtccg cttgttgtcc aagttcgatc cggcgtcccc
     3181 cgaattgccg ccccagcaac gcgcgcagtc cgatgcactc actccctcga cgcgacaatt
     3241 cactaccagc gtactagagt ttcttgcccc cacccaaacg cgcagactgg ggagccttgg
     3301 gaacaaaccg ctggcggtgg tcactgccgg cacacaggag cccagttggc tcaagcggca
     3361 ggacgagctg gccactctca cgtcctccaa cagcatccac cgtgtcgtga agggggcgac
     3421 gcacgaatca ctgttgtacg accggagcga cgcgcaagcg accagcgcgg ccatcgttga
     3481 ggtggtggcg gccgtgcgaa acgagcagcc gctggcgcag gaaaacccct aaccccctat
     3541 acctgaagtt cgcagcattt accttccacg cattcgcgtg aacaggccga agagtgagaa
     3601 aatagggttc acgacaacag cgtagaccct ctacgggagg cgaaagaaat gaacccgcca
     3661 cacctgagcg attttggcaa cctcgacacg gcaacgctca tgcggcactt gccgggtctg
     3721 aagttcccgg ctgagaagga gcaggtcgtt tcgactgccg agaacaatag agtaccccag
     3781 gaggcggtgc agcggatcag gcatacgagt cgccaacgct tcaacagtcc cgaagaagta
     3841 ttgcgggcgg tgcaaggcca ctagcaggtg acatagccat gggcaacgag aggctcataa
     3901 actacctgcg cttcgcagag cacggagccg agctggtcag ctcggagtcc accggagcct
     3961 tcgtactggc tgcagctggc ctactcgagg gacgaaaggc aaccacccac ccagccttcg
     4021 ccgagctact taagaagctg ggagtgaact acgttcagag ctattcggtt gaggacggca
     4081 ggttcctgac catgcaagga gtgtcgggag ggatcgacag catgctgaag ttggtggcca
     4141 agctaaaaag cgaggccgct gccaggcgcg cacagtacat aatcgagtac gatccccagc
     4201 cgcctttcgg aggagtcgac tggagcgagg cgaacggtga cgggctcgcg cagatgttag
     4261 aggagcaccg ggctgacttc gggcgtgagc tcgctgggcg gccagatctc ttccagaagt
     4321 tgttcatctg aagcacaccg ctgccgctcg tggtcagcgc aaaaccaata tccaggaaaa
     4381 tagaggagcc cgaatgacag cagaagaaca gaaccagaag accatcgcct tcgtcctgta
     4441 cccgggactt acacttttcg acctggcagg accgctccag gtcttcgcag gcgtaagcca
     4501 gctcgttcca cagtaccgga cggtggtagt tggagaaaac gcggagccgg cggacactga
     4561 tctacacgtg aagttgatcc ccacccacac cttcgaggag gtgcctcacc cttctgtcct
     4621 gctcgtgcct ggtaaccgca catccacgtt caaggccatg agcaacgagg cgctccggag
     4681 ctacgtgcgt tcggcggccg caaccgccga cgtggccggg tcgatctgca cgggggcgct
     4741 catcctggca tccgtcgggc tgctcgaagg taggcaagca actacccact gggtctacta
     4801 caagatcatg gaggagttcg gagccacgta cgtccgtaag agatgggtcg aggacggcaa
     4861 gttcatcagc tctgcgggtg tatccgcggg gatcgacatg gcgttgcatc tggcagcgag
     4921 gctgaccgac gaggagaccg cccggcgtgt acagcgctcg ctcggttacg atccgcatcc
     4981 ccccttcggg ggcatggact acggtcacct cggcgccctc tccgccataa gagccgggtt
     5041 gagcatggca gcgccaataa tagcggctcg accgaagcgg ctcacgaagc aaaaccgctg
     5101 aaaccaactg ggcagcctga gcaaggttgc ttgaaccccg tagaaaggag ctcctagtgg
     5161 agatatcgtc cgaggcatac gccaccctaa atcctactga gctggacaac ttcaagaacc
     5221 cgctgcggct gccgggtgag agcggagcta tgggcgtgct ggatgcctca gaccttccca
     5281 cgcgcatcac cgcccagaag gagagagtcg agattgtgcc cggcaagagc agcgaacttt
     5341 gggcttatcg cgccgagcgg gatggtaaga cctacgtcaa cccgaccttc agggtgcaga
     5401 agggaaagga gttctctgcg gaattcgcca atgacctcga cgaggagact acggtccact
     5461 ggcacgggct gcacgttgac tggcggatgg acggccaccc cttccggccg gtggctcccg
     5521 ggtctaccta ccgctacgtc ttccccctgc aagaccgggg tggaacctac tggtaccatc
     5581 cgcatcccca cgggggcact gctcgccaga cgtacgcagg actggccggc ttcttcctca
     5641 tagaagatga ggaacaacag cgcctcgacg aggcgctaga cctgagcttg ggcgaaacgg
     5701 acatcccctt gcttatccag gacaagatac tcggcgagag cgggaacttc gtctacgagc
     5761 cagacgcgat ggcggtggac atgggctacg agggggacgt catcctggta aacctgacgc
     5821 ccacccccta cctggaggta agctccagaa tctaccgctt caggctgctc aacgggtcca
     5881 acgcccgcac ctaccgggta gcctttacga agccagggga tgaagagttg ctgccctacc
     5941 aggtcatcgg cacagacggg ggcctcctgg accggccacg agcgactagg gaggtcttcc
     6001 tctcacccgg cgagcggata gacgtactct tggacctgag aaacttcgaa gtaagcgagg
     6061 agctcgtgct aaagagcttg tccttcgatc cgatgcaccg agaacatgag atgggcggga
     6121 gcatggatca tatggagcac atggacatgg gccaccatat ggacatgggg catcaccaca
     6181 tgcatcatca gatgggaccc gccaaccttc cagatggcag cgagttctac ctgttgaagc
     6241 ttgtgattgc agagaagacg ggctattcaa gaagcgtacc ggaggttctc tccgagatgc
     6301 cacagccgga cttgggcgga gcggacaccc gacctataac tatctctatg accaccgatg
     6361 atgaggggat gaggtggctc atcaacggcg agacccacac gatggacgag ttccccgtcg
     6421 tggtgcgaag gggtgcaaag gaggtctggg agatccataa cgatgagaaa agcatgcccc
     6481 accccgcgca ccttcacggc ttccagttca ggatcctgga gagggccggt acccccgagc
     6541 aggtcgcgca cctcgtagtg gaggagaaag gcagactggc tacagatgtg ggcttcaagg
     6601 atactgtgct cctttggcct ggggagaccg tgaagtgcgt catggatttc tctcacggct
     6661 tcgagggcga gcagatctac atgttccact gccacatcct cgagcacgag accactatga
     6721 tgctcaacgt caaaattgcg gactcgcaag ggtaggggaa tcggaggggc aagctgcgaa
     6781 cgcggaacct aaccgttgta gagagttccg cttacgcagc tggcgagggc tccgaagacc
     6841 ttccgcccgt gctcggttcg tccaggtcgc cctcgacccc cttgagtagc acggcgttga
     6901 cggcgacgat gatcgaagat acggacatca acagagcaga ccactccggc cttaacatga
     6961 tgccgtaggc aggatagagc acccctgcgg ctaccggtat cgcgagcacg ttgtacaccg
     7021 aggcccacac tagattttgc ttcatcttcc tgacagtggc cttggagagt accacggcgc
     7081 gggtgacgtc taaaggatcg ctcttcatca gaaccacctt cgccgtctcg atggccacgt
     7141 ccgtgccagc gccgatggcg atgccgatat ccgcctgcgc cagggcgggg gcgtcattga
     7201 ccccgtctcc gaccatcgca acgcgcttgc cctccctctg gagttgctgg acgtagcgcg
     7261 ccttgtccgc gggcagcacc tcggcgaaga cgcgctcgat gcctagttct cttgccacag
     7321 cttccgcggt tctcttgttg tcgcccgtga tcatgacagc ctctatgccc atctccttga
     7381 ggcgccgaag tgtatctctg gcgctcggct tgatagtatc cgccacggca acaagtccgg
     7441 acggctctcc gtccaccgcg acaaacatcg gcgtcttgcc ctccctggcg agcgcggttg
     7501 ctctgtcctc cagcttgaag acgtcaaccc cgctctcgtc catgagcttc aggttaccca
     7561 ccagaacagt ctgcccgttc acctcggcgc ggattccgtg tccggctatg gaatcaaagt
     7621 gcgcggcgtc tccgacctcg atacccctct cgcgggcgcc cgccactatc gcttcagcca
     7681 gaggatgctc ggagcccttc tcagccgggg ccacgaggcg cagaagatca tcctcgctcc
     7741 tgccgttgag cgtggataca tctgtgaggg agggctcacc ttctgttagg gtgcctgtct
     7801 tgtcgaggac gacggcgtct atgcgcgaga cgttctccag ggtagcggcg tctttgatga
     7861 ggatgttgtg cctggctcct atgccggtac ctaccgcaac ggcggtcggg gtcgcaagtc
     7921 caagggcgtc agggcaggcg atgacgacgg ccgagatcgc gaacgtcatc gccgtaatgg
     7981 cagcggcgcc acccaggaag taccaggcga ggaaggtcac gacacccgag ccgacggcga
     8041 ggatcaccag gtattcggcg gcgcggtcgg cgagcctctg tccgggtgcc ttggagttct
     8101 gcgccttctg gacgagatca acgatctggg ctagcgtggt ctcagccccg acggcggtag
     8161 ccctgaagcg gacgctgccg ctgcggttga tagagccggc gatcaccgcg tcgccggctc
     8221 gcttcgtgac tgggacgctc tcgcccgtca cgagcgcctc gtctatgctc gtctccccct
     8281 ctgtgacttc gccgtccacg ggcaccttgt caccaggtct caagatgacg atgtcattta
     8341 gacgcacctc gctgctcgga atctccacct cttcgccgtc cctcattacc gttgcctgtg
     8401 ggggcaccag gtcgaagaga gagcgcaggg cgtcgctggt gccgcgcctg gagcgcatct
     8461 ccatccagtg gccgaagagg acgaaggtga cgagcatggc cgccgcctcg tagaaggtct
     8521 ccccgccgag gaaggtgatc aggacgctga agatgtaggc cgcgagcacg ccggtggcga
     8581 tgagcacgct catgttgagg gtgcgcctgc gcaaggaatg gtatgagccg gagatgaaga
     8641 tccaaccgca atagaagacg accggtgttg agaggagaag catgattgcg ttctcaccca
     8701 acccgaaggt cggtagccgt aagccaaata gatttatgcc gagcggcgag taaagcacgg
     8761 tcgggatcgt aaggatcaag gcgacgaaga acctgttgcg catgtggcgc tccatcgctt
     8821 tcgccatggc agggtctgac atgtcgtggt ccatgtggtc gccatgatcc gctggggttg
     8881 cgggcatctc gtactgcatg cggtcgtgct ttgttcccat agagatgggc tgcgcatcta
     8941 cgccgtg
//
LOCUS       DATAIR010000805         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_273725, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000805 DATAIR010000000
VERSION     DATAIR010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273725"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VHM69_19130"
     CDS             complement(<1..614)
                     /locus_tag="VHM69_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742561.1"
                     /translation="MFDHIRRRLTMGYVGILALILLIFGVVVVISFQKQVTFQQDKLL
                     DQKVKSLVDGSLSGTDKNAILEDPNSYGWIIFTPDGRMLGQNDSAVSLGLPSEQLARR
                     SLHQGGIVSTTIKGPEGEVRVASSPALQSGTVVVLQVARSPAVVQGIVDRLVIVLFTV
                     GLGALILAAAGGLFMSRRAMRPVQEAFGRQRAFIADASHELKTPL"
     gene            complement(614..1303)
                     /locus_tag="VHM69_19135"
     CDS             complement(614..1303)
                     /locus_tag="VHM69_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742562.1"
                     /translation="MRILVVEDEERLARLISRVLKEEGYAVETESNGRQALVRALAGE
                     YDLLIVDWMLPELSGVRMVKRLRAAEVGTPAIMLTARDQIEDRVEGLDAGADDYLPKP
                     FAFPELLARVRALTRRPRGEESDRAVLRAGDVTLDPVRHVVRHGGEAVDLTAKEFALL
                     ATLLQRPGQVFTRPVLLDTVWGASPEVYANVVDLYVSYLRKKLDRDGGASHIRTVRGV
                     GYTFEPQPGPR"
     gene            1445..2293
                     /locus_tag="VHM69_19140"
     CDS             1445..2293
                     /locus_tag="VHM69_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX2742563.1"
                     /translation="MATRPRTYRETEAPGLLAAYMAHIGRGELLCHREEVELGRRART
                     GDREARRRLIEKNLRLSVSVAKKYRGQGLPFEDLIQEGNIGLMKAVDKFDPEKGNRFS
                     TYATWWIRQSIQRALGDKGREIRLPAHMNEKLRKIRKAHGELYAELHRAPTMEEISVR
                     LGWEADKVSAILQSVPEISSLDRPLGPEADARGLGDLVEEQEPHGPDLGESLREAMRQ
                     LPERERQVLVRRYGLDRGAKATVRELSARLGISEKAVRRAQRRAEQLLKAFLEPGTRG
                     LGEVTV"
     gene            2290..>3367
                     /locus_tag="VHM69_19145"
     CDS             2290..>3367
                     /locus_tag="VHM69_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2742564.1"
                     /translation="MKSYTLQRSFLVPLTVLAILVFVLGVIYLDPGTGNTQAADPVKS
                     APANIPADEPVARVASVLSPSVVQINVSGIQQTPFGAQKEEGIGSGVIYRSDGYIITN
                     NHVVEGTSEVEVAFADGTTERGRVVGTDPTTDIAVVKVDRNNLPAASFASGDPMVGQL
                     AVAAGSPSGFESTVTSGIISGIGREIPAEYTGGRQDDSLVDLIQTDAAISPGNSGGAL
                     ANRKGQVIGINVAYLPPSQTGAENIGFAIPSDTAVSVADQLINNGEAVHPYLGVYLSD
                     LTTETASRFGSPVDSGALVERVESGGPADDAGVRRGDVVTAAGSEEIRSSGDLISALR
                     NFRPGDTIDLTVLRNGEKTTLQANL"
BASE COUNT          664 a         1081 c         1064 g          558 t
ORIGIN      
        1 agtggagtct tcagctcgtg ggaggcgtcg gcgatgaacg cgcgctgcct gccgaacgct
       61 tcctgcacgg gacgcatcgc gcggcgcgac atgaacaggc cacccgccgc cgccaggatc
      121 agcgccccta gcccgaccgt gaacaggacg atgaccagcc ggtctactat cccctgtact
      181 accgcgggcg aacgcgctac ctgcaatacg accaccgtcc ccgactgcag cgcaggcgaa
      241 ctcgccaccc gcacctcacc ctcgggtccc tttatcgtcg tggagacgat cccgccttgg
      301 tgcagtgatc tccgagccag ttgctcggaa ggaagcccca gggaaacagc ggaatcattc
      361 tgccccagca tgcgtccatc cggcgtaaag atgatccagc cgtagctgtt gggatcttcg
      421 agtatggcgt tcttgtccgt gccggacagc gatccgtcca cgaggctttt taccttctga
      481 tccaatagtt tgtcctgctg aaaagtgacc tgcttctgga agctgatcac cactacgacg
      541 ccgaagatca gcaggatgag cgcgagtata cccacgtagc ccatcgtcag gcgccgccgg
      601 atgtggtcga acatcagcgc gggcctggtt gtggctcgaa cgtatatccg acgccgcgaa
      661 cggtgcggat gtgcgaagcg ccgccgtcac ggtcgagctt tttgcgcaaa tacgagacgt
      721 agaggtcaac gacgttggcg tagacttcgg gagaagcgcc ccacacagta tcgagcagta
      781 ccgggcgggt gaagacctgt ccgggacgct ggagcagggt ggcgagtagc gcgaactcct
      841 tggccgtcag gtcaacggcc tctcccccgt ggcggaccac gtggcgcacg gggtcgagcg
      901 taacgtcgcc ggccctgagg acagcacggt cgctctcttc gccccgggga cggcgggtga
      961 gcgcccgcac gcgggcgagc agctcgggga aggcgaaggg cttcgggagg taatcgtccg
     1021 cgcccgcgtc caggccctcc acacggtctt cgatctggtc cctcgccgta agcatgatcg
     1081 cgggcgtccc gacctccgcg gccctgagcc tcttcaccat ccggactccg ctcaactcgg
     1141 ggagcatcca gtctacgatg aggaggtcgt attcgccggc cagtgcccgc actagcgcct
     1201 gtctgccgtt ggactccgtc tccacggcat agccctcctc tttgaggacc cgtgagatca
     1261 ggcgcgccag cctctcctca tcttccacga ccagtatccg catctccctc tcattatcgc
     1321 cgctgtgcgg cgagtgtgtc ctgtttgagt cctttcctgt gaaaaccctc atggaccgca
     1381 aagacgcttc acatattgga agggcaggct ttggagcaga gatcccgcac cgagaggagg
     1441 agcgttggcg acacgtccga gaacctacag ggagacagaa gcaccggggc tcctcgcggc
     1501 atatatggcc cacatcggca ggggcgagct actgtgtcac agggaggagg tcgagcttgg
     1561 ccgcagggca aggacgggcg accgtgaggc ccgccggcgg ctcatcgaga agaacctgag
     1621 gctgtccgtc tcggtggcca agaagtatcg cggtcagggt ctgcccttcg aggacctcat
     1681 ccaggagggt aacatcgggc tcatgaaggc ggtggacaag tttgaccccg agaagggcaa
     1741 ccgcttctcg acatacgcga cgtggtggat ccgccagtcg atccagcgtg ccctcggaga
     1801 taaggggcgt gagatccggc tcccagccca catgaacgag aagcttcgca agatccgcaa
     1861 agcacacggc gagctgtatg cggagctcca ccgcgctcct actatggagg agatctcggt
     1921 ccggctagga tgggaagcag acaaggtcag cgccattctc caatccgtac ctgagatctc
     1981 gagcctcgac cggccactcg gtcccgaggc tgacgcccgc ggactcggag acctcgtgga
     2041 ggaacaggag cctcacggcc ctgatctcgg ggagagcctc cgagaggcca tgcgccaact
     2101 gcccgagcgc gaacgccagg tcctggtgcg ccgctacgga ctcgacagag gcgcaaaagc
     2161 gaccgtgcgc gagctctcgg ccaggctcgg catctcggag aaagcggtca ggcgggcgca
     2221 acgcagggct gaacaattac tcaaggcatt cctcgaacct ggaacgcgcg gtctcgggga
     2281 ggtaacggta tgaagagcta cacattacag cgctccttcc tggtgccgct aacggtcctg
     2341 gcgatacttg tgttcgtgct cggcgtcatc tacctcgatc cgggaacggg caatacgcag
     2401 gccgccgatc cggtcaagtc ggccccggca aacatccctg ccgatgagcc cgtggccagg
     2461 gtagcctccg tgctgagtcc ttcagtcgtg cagatcaacg tctccgggat ccagcagacg
     2521 ccattcgggg ctcagaaaga agaagggatc gggagcggtg tgatctaccg ctccgatgga
     2581 tacatcatca ccaacaacca cgtcgtcgag ggcacgagcg aggtggaggt cgccttcgct
     2641 gacggcacca ccgagcgcgg gcgagtcgtc ggcacggacc cgacgacgga catcgccgtc
     2701 gtcaaggtgg accgtaataa cctgcctgcc gcgagcttcg cgagtggaga tcccatggta
     2761 ggccagcttg cggtcgccgc aggcagccct tccggcttcg agtcaaccgt cacatccggc
     2821 atcatcagcg gcataggacg cgagatcccc gccgagtaca ccggcgggag gcaggacgac
     2881 tctctggtgg acctgatcca gaccgacgcg gcgatctccc cgggcaactc cggcggcgcc
     2941 cttgcgaacc gcaagggcca ggtaataggt ataaacgtcg cctacctgcc gccgagtcag
     3001 accggggctg agaacatcgg cttcgccata ccttctgaca cagcggtctc cgtggcggac
     3061 cagctcatca acaacggcga ggccgtccat ccctacctcg gcgtatacct gtctgacctc
     3121 accaccgaga ccgcgagcag gttcggaagt cccgtagatt caggggcgct ggtcgaaagg
     3181 gtagaaagtg gcggtccggc ggatgacgcc ggagtacggc gcggcgacgt cgtaacggcc
     3241 gcaggttcgg aggagatccg cagctcaggc gacctcatct cggcgcttcg gaacttcagg
     3301 ccaggcgata cgattgacct tacggtcctc aggaacggcg agaagaccac gctccaggcg
     3361 aacctcg
//
LOCUS       DATAIR010000806         5490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_367588, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000806 DATAIR010000000
VERSION     DATAIR010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367588"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /locus_tag="VHM69_19150"
     CDS             complement(<1..2043)
                     /locus_tag="VHM69_19150"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX2742565.1"
                     /translation="MRLEIPVGERSIMLETGKLARQAGGAVTARLGDTTILSTATRSK
                     APRPGATFLPLSVDIEERMSSAGKIPGGFIKREGRPSERAILTSRLTDRPLRPLFPKN
                     YHYEIQVVGTVLVADQDTPYDTVSMVGASAALALSDLPFAGPIGAVRVGRSLDGEFIL
                     NPTYAQIEESDLDLVVAGTKEAITMVEAGANEVPENVIVEALQVAQEVVRGQAEAIEE
                     WAGEYGKEKQKVEDPEENPFLAEFREKYLDRVKEGIVQTDRRARHDALDELREELIEG
                     RDEEEIPAISVALSIVEKEAFRELYIKDRKRTDLRGLDEIRPTSAEVQVLPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPQTGKRYMHHYNFPPFSTGETGRLGSPRRRE
                     IGHGALAERALVPVIPSEDEFPYALRIISEVLESNGSSSMASVCGSTLALMDGGVPIK
                     APVAGVAMGLVKEGDDYVILSDIQGLEDHMGDMDFKVAGTTDGITALQMDMKITGVSA
                     QLLQEALEQARAGRLEILEILRGAIPEPRSEVSDYAPRVEVLKIPKDKIGMLIGPGGK
                     TINALQEQFGVNISVEDDGTVFVAGEGSVAKDAAGAINGMMKDVEAGEIYTGKVVKTT
                     NFGAFVELTPGRDGLVHISRLAPGKQRVERVEDVVTEGDRIKVRVLEIDKQNRISLE"
     gene            complement(2079..2345)
                     /gene="rpsO"
                     /locus_tag="VHM69_19155"
     CDS             complement(2079..2345)
                     /gene="rpsO"
                     /locus_tag="VHM69_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2742566.1"
                     /translation="MAVVEHKEEIIEEHRTHEGDTGSPEVQVAVLTRRIAHLTEHLRE
                     HKHDYHSRRGLLKLVGRRRRLLKYLQNKDVERYRALIAKLGLRR"
     gene            complement(2491..3300)
                     /locus_tag="VHM69_19160"
     CDS             complement(2491..3300)
                     /locus_tag="VHM69_19160"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX2742567.1"
                     /translation="MRSGGGATASDRVEAGQAPESATGLPEAAGGMSEAQIAMDDFDR
                     KIVKTAELGIRADDVRRGAARAQEVTTRFGGSIISSQTYRVNDSVYADLVISVPSEEF
                     EQSLDELRGLGKEVTTDTISGEDVTEEYVDLQSRERNLLAAEEALLRLYDRAEDVQDA
                     LLVERELTDVRGQIEQVQGRIQYLKQRSDYAQISLGIRPVASPPKPPPAWDPTLVVAR
                     AWSASLAILQTLATAILSTLVFGWWLVPLLLAGTWWLRRRLRRSSFVGSEP"
     gene            complement(3523..4419)
                     /locus_tag="VHM69_19165"
     CDS             complement(3523..4419)
                     /locus_tag="VHM69_19165"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX2742568.1"
                     /translation="MRGVVVALGNFDGVHLGHQVVLRRAEEEGRQRGIKVVAATFHPH
                     PRSVLGGGDPPRLLTPLELRREALLAYGADEVLVVAFDLELSKKTPEQFVQDVLVGEI
                     GAEVVVVGENFRFGHKAAGDFRDLRRLMREAGGEAVAVHVRGAGREGGISSTRIRTLV
                     AEGEVSEAAGLLGRSYVLRGEVVVGDMRGRSIGFPTANVLPDADAVIPARGVYAGFVF
                     VGDKRYAACTNVGVAPTFGRAESRVEAHLMDFEGDLYGRIVDVSFAKRIREERRFSGV
                     DELTAQIRRDVEEARLFTGSIG"
     gene            complement(4407..5282)
                     /gene="truB"
                     /locus_tag="VHM69_19170"
     CDS             complement(4407..5282)
                     /gene="truB"
                     /locus_tag="VHM69_19170"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2742569.1"
                     /translation="MTNGALSGGILLDKPPGITSARAVSRVKRLLPKQTRIGHTGTLD
                     PLASGLLVLLIGRATRLSRYVTDLDKAYTATARFGAVSDTLDAEGEITTLEAPFPSEG
                     AFHNALPNFTGDLLQVPPMASALKREGVRLYDLHRRGISVEREARPVTVHSFELTRLD
                     PNEESATFEISCSSGTYVRTLIHDLAASLGSGAYLTALRRTSVGHLSVDEATGDLDPD
                     TIDNHIIPPMRLVSHLPEVSIPLDIGRGRVSHGRRLESAGISGSYRVMSEGELLAVYR
                     DEGEEARAEVVLCAE"
     gene            complement(5279..>5490)
                     /locus_tag="VHM69_19175"
     CDS             complement(5279..>5490)
                     /locus_tag="VHM69_19175"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX2742570.1"
                     /translation="DVVAHLREVPQGTKGSLRSETVDVGEIARHFGGGGHRLAAGYTT
                     PRRPQEAKVELLERLEGVLDLGGGR"
BASE COUNT          948 a         1825 c         1618 g         1099 t
ORIGIN      
        1 ctccaggctg atgcggttct gcttgtcgat ctcgagtacg cgcaccttga tgcggtcgcc
       61 ctcggtcacg acatcctcga cccgctcgac ccgctgcttg ccgggggcca ggcgcgagat
      121 gtgcacgagc ccgtcgcgac ctggcgtgag ctcgacgaag gccccgaagt tggtggtctt
      181 tacgaccttg cccgtgtaga tctcaccggc ctcgacatcc ttcatcattc cgtttatggc
      241 gccggccgcg tcctttgcga cgctcccctc gccggcgacg aagaccgtcc cgtcgtcctc
      301 gacggagata ttcaccccga actgctcctg cagggcgttg atggtcttcc cgccggggcc
      361 tatgagcata ccgatcttgt ctttcggtat cttcagtacc tcgaccctcg gcgcgtagtc
      421 ggagacctcg gagcgcggct cgggtatggc gccgcgcagg atctcgagta tctctagtct
      481 tcctgccctg gcctgttcca acgcctcctg caacagctgg gcggagacgc cggtgatctt
      541 catgtccatc tgcagcgctg ttatgccgtc tgtcgtgccg gcgaccttga agtccatgtc
      601 gcccatgtgg tcctcgaggc cctggatgtc cgagaggatg acgtaatcgt cgccctcctt
      661 gacaaggccc atcgccacgc cggccaccgg ggccttgatc ggcacgcctc cgtccatcag
      721 cgccagcgtc gagccgcaca cgctcgccat cgaggaggaa ccgttcgatt cgagcacctc
      781 ggatattatg cgtagagcgt aggggaattc atcttctgaa gggatgacgg gtacgagagc
      841 acgctcggcg agtgcgccgt gcccgatctc acgccgcctc gggctgccga gccggcccgt
      901 ctctcctgtg gagaacggcg ggaagttgta atggtgcatg tagcgcttgc ctgtctgcgg
      961 ctcgatggta tccagccgct gcgcgagtcc aaggtcggcc agagccagcg agctcaagac
     1021 ctgcgtctcg ccacgggtga agaggcccgt gccgtgcacc cgcgggagca cctgtacttc
     1081 ggctgaggtt ggccttatct cgtcgaggcc gcgcaggtcc gtgcgcttgc ggtccttgat
     1141 gtagagctcg cggaaagcct ccttctccac aatcgaaaga gcgaccgaga tggcggggat
     1201 ctcctcctcg tcacggccct cgatcaactc ctcgcgcagc tcgtcgagcg cgtcgtggcg
     1261 agccctgcgg tctgtctgga cgatgccttc cttgactcgg tccaggtact tctcacggaa
     1321 ctcagcgagg aaggggttct cctcgggatc ctctaccttc tgcttctcct tgccgtactc
     1381 accagcccac tcttctatag cctcggcctg gccgcggact acctcctggg cgacctgcag
     1441 agcctccact atcacgttct cggggacttc attcgccccc gcctcgacca tggtaatggc
     1501 ttccttggtt ccggcaacta ccagatccag gtcgctctcc tctatctgtg cgtaggtcgg
     1561 gttcaggatg aactcgccgt cgagcgaacg cccgacgcgc acggcaccaa taggtccggc
     1621 gaaaggcagg tccgagagcg ccagcgctgc cgaagcaccg accatgctca cggtgtcata
     1681 aggcgtatcc tggtcggcga ccagaactgt acctacgacc tgtatctcgt agtgatagtt
     1741 cttcgggaag agcggcctca ggggtctgtc cgtgagacgg gaggtcagga tagcccgctc
     1801 ggagggcctc ccctctcgct tgatgaatcc gcctgggatc ttgccagccg aggacatgcg
     1861 ctcctcgatg tccacgctga ggggcaggaa cgtcgcgcct gggcgaggtg ccttcgagcg
     1921 cgtcgcggtt gaaagtatag tcgtgtcgcc gagcctggcc gttacggcgc cgccagcttg
     1981 cctggctagc ttcccggttt cgagcatgat cgaacgctcg cctacgggta tctctagacg
     2041 catattatgt tctcctcttg tttctgttgt tacccggact atcggcgtag gccgagcttg
     2101 gcgatgagcg cccggtagcg ctcgacatct ttgttctgca agtatttgag cagccggcgg
     2161 cgtctgccga ccaacttcag caatccccgc cgcgaatggt agtcgtgctt gtgctcgcgc
     2221 aggtgctccg taaggtgcgc tatccgcctg gtgagcaccg cgacctgcac ctcgggcgac
     2281 cccgtgtcac cctcgtgcgt ccggtgctcc tcaatgatct cttctttgtg ctcaactaca
     2341 gccaacttct tccttctcct ctttgctcac ttctctcttg tttaactcgt tcgacccctt
     2401 atgctaccac atgcatcaaa tagtatcgtc agctattgct atcgtgaaaa cgacctgaaa
     2461 tacaggacgc gcacgtgttg aagcaggctt tcagggctct gatccgacga agctcgaacg
     2521 tctgagcctg cgcctgagcc accacgtccc tgccaggagc agcggtacga gccaccagcc
     2581 gaagactagc gtcgagagga tcgcggtggc cagagtctgg aggatagcga gtgacgcgct
     2641 ccacgcccga gccactacca gcgtcggatc ccaggccggc ggaggcttcg gaggactggc
     2701 tacgggcctg atgccgaggg aaatctgggc gtagtcgctt ctctgcttga ggtattggat
     2761 cctaccctgt acctgctcga tctgtccgcg tacatcagta agctcgcgct ctaccaggag
     2821 cgcgtcttgt acgtcctcag ccctgtcgta gaggcggagc agggcctcct cagccgcaag
     2881 gaggttcctc tccctggact gaaggtcgac atactcttcg gtcacgtcct ctccagaaat
     2941 ggtatcggtc gtaacctctt taccgagccc ccttagctcg tcgagcgact gctcgaactc
     3001 ctccgaggga acggagatga ccaggtcagc gtagacggaa tcgttcacgc gataggtctg
     3061 cgagctgatg atgctccctc cgaaccgcgt cgtgacctct tgcgccctcg cggcaccgcg
     3121 gcgaacatca tcagcccgta tgccgagctc cgccgtcttc acgatctttc ggtcgaagtc
     3181 gtccatcgcg atttgggctt cggacatacc gcccgcggcc tccggcaacc cggtagctga
     3241 ctcaggcgcc tgccccgcct caacccggtc tgaggcggtc gctccgccgc ccgatctcac
     3301 ctcttctccg ccacccgcgc aggctgggac aagtagtacg aagagcaagt atatgcacgc
     3361 tctcgcgcaa accccggacc gcatgaaaac ctcccatcgt gtgcgacacg tctatcctct
     3421 ttacacgcgt cgacacgtat gggttccgtg aaagggaagt acacgccgga tagaggactg
     3481 gcaacgttcg gcaaagaggc gacgggaatc gcagatccag tgctacccta tgctcccggt
     3541 gaaaaggcgg gcctcctcaa cgtcgcggcg gatctgcgcc gtgagctcat cgacgcccga
     3601 gaatcgtcgc tcctccctga tgcgcttcgc gaagctgaca tccactatgc gcccgtagag
     3661 atctccttcg aagtccatga gatgggcctc gacgcggctc tccgcccgac caaaggtagg
     3721 agcgactccg acgttcgtac atgccgcgta cctcttgtct ccaacgaaga cgaaaccggc
     3781 gtagacgccg cgtgcgggaa tgaccgcatc agcatcgggt aacacgttgg ccgtcgggaa
     3841 gccgattgac cttccgcgca tgtctccgac caccacctcg cccctgagta cgtaggatcg
     3901 tcccagcaga ccggcagcct cggacacttc gccttccgcg acaagcgtcc ggatccgtgt
     3961 cgagctgatc ccaccctccc tcccggcccc gcgtacgtga accgcgacgg cctccccgcc
     4021 cgcttcccgc atgagccgcc tgaggtcacg aaagtcgccc gcggccttgt gcccgaaccg
     4081 gaagttctca ccaaccacga ccacctcggc cccgatctcc ccgaccagca catcctgtac
     4141 gaactgctca ggggtctttt tggagagctc cagatcgaaa gctaccacca acacttcatc
     4201 ggccccgtat gcgagcagcg cctcacgccg caactcgagc ggcgtgagaa gcctcggcgg
     4261 atcccctcca cccagtaccg accgcggatg cggatggaac gtggccgcaa cgaccttgat
     4321 acctctctgc cgtccttcct cctcagcccg ccttaagacc acctgatgac cgaggtgcac
     4381 gccgtcgaag ttcccgaggg caacgactac tccgcgcata acacaacctc ggcccgcgcc
     4441 tcctcgccct cgtccctgta caccgccagc aactcgccct cactcatcac ccgatagctg
     4501 cccgaaatcc ccgcactctc taaacgcctg ccgtggctta cccgcccacg cccaatatcc
     4561 aaaggaatag atacctcagg caggtgcgaa accaaccgca tcggaggtat tatgtggtta
     4621 tctattgtat cggggtcgag gtctcccgtg gcttcatcga cgctgaggtg tccgacgcta
     4681 gtacggcgca gggcggtcag gtaggcacct gagccgaggg aggcggcgag gtcgtgtatc
     4741 agggtacgca cgtaggttcc gctgctgcag gagatctcga acgtggcgct ctcttcgttc
     4801 gggtcgaggc gggtgagttc gaagctgtgg accgtaaccg ggcgtgcctc tcgctcgacg
     4861 ctgatgccgc gccggtggag gtcgtagaga cggacgccct cgcgcttgag ggctgaggcc
     4921 atgggtggga cctggagtag gtcgcctgta aagttgggga gcgcgttgtg gaaagcgcct
     4981 tctgacggga aaggggcttc gagggtggtg atctcaccct ccgcgtcgag cgtgtcggag
     5041 acggcgccga agcgggctgt tgctgtgtag gccttgtcga ggtcagtcac gtagcgcgag
     5101 agacgtgtgg cgcgcccgat gagcagtaca agaaggccag aggccagggg gtcgagggtg
     5161 cccgtgtgcc ctatcctggt ctgcttcggc aggaggcgct tgaccctgga gacggccctg
     5221 gcgctcgtaa tgccgggcgg cttatccagc aggatgccgc ccgatagagc cccgttggtt
     5281 atcgtccgcc cccgagatcc aggacccctt caagcctttc aagaagctcg actttcgctt
     5341 cctggggacg gcgaggggtg gtgtatcctg cggcgagcct gtgccccccg cctccgaagt
     5401 ggcgtgcgat ctcccctacg tcgacagtct cggagcggag ggaacctttc gtaccctgcg
     5461 gcacctcgcg cagatgggcc acgacgtcga
//
LOCUS       DATAIR010000807         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_367882, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000807 DATAIR010000000
VERSION     DATAIR010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367882"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VHM69_19180"
     CDS             complement(<1..95)
                     /locus_tag="VHM69_19180"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX2742571.1"
                     /translation="MNEERGFTLWFTGLSGSGKTTISEIVEHELR"
     gene            complement(162..1463)
                     /locus_tag="VHM69_19185"
     CDS             complement(162..1463)
                     /locus_tag="VHM69_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX2742572.1"
                     /translation="MPDLPNILYIHSHDTGRWVRPYGHSVRTPNIQRLAEEGVLFRKA
                     FCAAPTCSGSRACLLTGQYAHSNGMVGLAHRGFSLKDYRHHIVHTLRELGYWSALIGE
                     QHISKKPDIIGYDEVIKISTSHTDDVVPVTLDLLERASDRPFFLSVGFFETHREWFRP
                     SSPKKANYVMPPPNLPDAPETRRDMAAFHESARSLDRGIGAVLDALDAEGLADDTLVI
                     CTTDHGLAFPGAKATLYDRGLGVMLILRGPGGFTGGRASDALVSHIDLFPTVCDLTGI
                     EPPEWLQGESLLPLASGEVEEVRDAIFAEKTYHVAYEPERCVRTQRWKYIQRFGDRSR
                     PVLPNTDDGPSKDLLLSHGWGEREIPAEQLYDVILDPNETCNLAGDPAHADTLEELRG
                     RLKAWMVKTEDPLLDGDVPAPKGAELNDPDGLSPTEPTTVV"
     gene            complement(1467..2339)
                     /locus_tag="VHM69_19190"
     CDS             complement(1467..2339)
                     /locus_tag="VHM69_19190"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEX2742573.1"
                     /translation="MKPELSYLICATPRSGSTLLCAALDDTGIAGHPEEHFEVLLETG
                     RPRQPRDYFQRSNDPEVWALLDDPEFEDVLGEYGGRYSDHQARRDPSWRPPAFETLVD
                     EALKKGTTENGVLGTKIMWAYLRDFVRLARRTPRLKSVPPRDVPASVLPNLRRFVWIH
                     RADTTRQAVSLWKALQSQQWRRDSDEELGGRGLRFSFAAVDHLKVRIDEHNAAWQNFF
                     EECGVEPMEVVYEELVEDYEGTVLWLLDGIGISIPENFGVTEPKMRRQADDLSEEWVR
                     LYNERAAVKTVQKG"
BASE COUNT          409 a          757 c          758 g          474 t
ORIGIN      
        1 tcgcgcagct cgtgctcgac gatctcggag atggtggtct tccccgatcc ggagagaccc
       61 gtgaaccaca gggtgaatcc gcgctcttcg ttcatatacg ttcctttcgg tagattgatg
      121 atctctactg tccgtagttt acatacccct ttgagccccg cttacacgac ggtcgtgggc
      181 tcggtggggg atagaccgtc agggtcgttg agctcggctc ctttgggcgc aggcacgtct
      241 ccgtcgagga gagggtcttc cgtcttcacc atccacgcct tgagccgccc gcgcaactct
      301 tccagagtat ccgcgtgggc ggggtctccg gcgaggttgc acgtctcgtt ggggtccagt
      361 atgacgtcgt agagttgctc agcggggatc tcacgctcgc cccatccatg cgagagtagc
      421 aggtccttgc tcgggccgtc gtcggtgttc ggcaagacgg gccttgatcg gtcgccaaag
      481 cgttggatgt acttccagcg ctgcgttcgg acgcaccgtt cgggctcgta ggcgacgtgg
      541 taggtcttct cggcgaagat ggcgtccctg acctcctcga cctcaccgct cgcgagcgga
      601 agtagcgact cgccctgcag ccattccggc ggctcgattc cggtgagatc acagacggtc
      661 gggaagaggt ctatgtggga gacgagagcg tcgctcgccc tgccgcccgt gaatccgcct
      721 ggcccgcgca ggatgagcat gacgccgagg cccctgtcgt agagtgtggc cttcgcgccg
      781 gggaaggcga ggccgtggtc cgtcgtgcaa atgacgagcg tgtcgtcggc caacccctcc
      841 gcatcgagtg cgtcgaggac ggcgcctatt cccctgtcga gcgagcgggc gctctcgtgg
      901 aaggcggcca tgtcacggcg cgtctcgggg gcgtcgggca gattgggcgg aggcatgacg
      961 tagttggcct tcttcgggga actgggacgg aaccactctc ggtgggtctc gaagaaaccg
     1021 acggagagga agaacggcct gtcgctcgca cgttcgagca ggtcgagcgt taccggcacg
     1081 acgtcgtcgg tgtggctggt gctgatcttg atgacctcgt cgtagcctat gatgtcaggc
     1141 ttcttggaga tgtgctgctc gccgatgagc gccgaccagt agccgagctc gcgcagagtg
     1201 tggacgatgt ggtgcctgta gtccttgagc gagaacccgc ggtgcgcgag cccgaccatc
     1261 ccgttggagt gcgcgtactg tcccgtgagt aaacaggcac ggctccccga acacgtcggg
     1321 gcggcgcaga acgccttgcg gaatagcacg ccctcctcgg cgagacgctg gatgttcggg
     1381 gtcctgacgg agtggccgta cggcctgacc caccgccccg tatcgtggga gtgaatgtag
     1441 aggatgttgg gaagatccgg caattcctat cccttctgga cggttttaac ggccgcgcgt
     1501 tcgttgtaca gccggaccca ctcctctgag agatcatccg cctgccgcct cattttaggc
     1561 tcggtgacac cgaaattctc agggatagag atcccgatac cgtcaagaag ccacaagacg
     1621 gtcccctcat agtcttcgac taactcctcg tagacaacct ccatgggctc cacgccgcac
     1681 tcttcgaaga agttctgcca ggcggcgttg tgctcgtcta tgcgcacctt taaatggtca
     1741 acggccgcga agctgaaacg cagaccacgc ccgccgagtt cttcatcgga gtccctgcgc
     1801 cactgctggc tctggagcgc cttccacagg gagaccgcct ggcgggtggt gtccgcgcga
     1861 tggatccaga cgaaccgtcg caggttgggc aggaccgagg cgggcacatc gcggggagga
     1921 acgcttttca gcctgggcgt gcgtcgcgcc aggcggacga agtcgcgcaa ataagcccac
     1981 atgatcttgg tccccagcac gccgttctcc gtcgtccctt tctttagcgc ctcatccacc
     2041 agcgtctcga aagccggtgg acgccaggaa gggtccctcc tcgcctgatg atctgagtac
     2101 ctaccaccgt actcgccgag cacgtcctcg aactcggggt cgtcgaggag cgcccatacc
     2161 tccggatcgt tggagcgctg gaagtagtcg cgcggttgcc tgggccgccc cgtctccagc
     2221 agaacctcga aatgctcctc gggatggccc gctatccccg tgtcgtcgag cgccgcgcac
     2281 agcagcgtgc tcccgctacg aggcgtcgcg cagatcaaat acgacagctc aggcttcacc
     2341 gcgccaccgc ctcagttagt ccgcgatcac tcatcaagcc tcctcattac gtcaccaa
//
LOCUS       DATAIR010000808        10279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_170479, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000808 DATAIR010000000
VERSION     DATAIR010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170479"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHM69_19195"
     CDS             <1..325
                     /locus_tag="VHM69_19195"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2742574.1"
                     /translation="PTANVIATFSEAMNEASVEKTTANDLPTTFTLKKKGTTKTLAAT
                     VVYSEPTATTFKAGLDPTRKLRAGATYIATVSSVATDLVGNALDQNPSLTGNQPKTWT
                     FTVKP"
     gene            449..2386
                     /locus_tag="VHM69_19200"
     CDS             449..2386
                     /locus_tag="VHM69_19200"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2742575.1"
                     /translation="MTSTSVVKKLREHRWAAVIFAALLLSTLALAFALTAGPARAIDD
                     DPCPPICDPPENNPPSVAATNPSSTANEGQTASNDGTFSDADGNATVTLSASVGSVTK
                     NLNGTWSWSFNTSDDPDQSQTVTITADDGTETATTTFSLTVNNVAPTVNLQSGSFSSV
                     QEGGSYANGYTSYGYNLSVSDPGNDTRTLNTGCGANGSEGFESTTQVWCGFFDGPGSS
                     TVSATATDSDGASRTASVLVNVTNMHPVAQVDATTSINEGSTATVSLIKGGFSYQDGE
                     TDTNAGFRYAFDCNNGPLTTYNYANSGTANSVNCPTTDNGTKTVRAKIMDKDGGSTEY
                     TKSITVNNVSPTATFNTPTSVNQGNNFTISLGSVSDPSSNDTLTYAFDCGDGSGYGPP
                     SSTPSKGDCPALDKPTLTVKGKVMDDDGGSNEYTANVTVNNVFPTGTIQVNGGAPATN
                     NAVVNLALSANDPLPGSNVDQMRFRNENTTTWQAWEDYATSRAWQLSSGDGTKTVFVE
                     YKDNAGNASQGVISDQILLDTTDFTAPTVLKWTPTTKRVSPTANVIATFSEAMNEASV
                     EKINASGLPTTFTLKKKGTTKTLAATVVYSEPTATTFKAVLNPSRNLRAGATYIATVT
                     SAATDFVGNPLVKKTWTFTVK"
     gene            complement(2510..3880)
                     /locus_tag="VHM69_19205"
     CDS             complement(2510..3880)
                     /locus_tag="VHM69_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742576.1"
                     /translation="MFRSTASKVMWVGRATVFLVGLSVILALIFGAASAALGANGDSF
                     ILGTTANTATKVTGLVGSIADTTKAALLVRNNRGGAALDLRVGSPTVPANDVVPMKVN
                     STKKVDNLNADEIDGLDSTNLQAKYAQTVVVAKSGGDFTSVQAALDSITDASGSKHYL
                     VWIGPGTYDGRVQMKPFVDIEGAGELATTITAPGGTGFAAGSSVVSGANDAELRHLTV
                     ENTGGGTDAMAIFNASASPRLSNVTATAGGAVDNKGVVNASSSPTMTDVTATASGGNS
                     AFGVANSGGSSPTMTDVSAKGSGADYNVGINNAGSFPTMTDVSAKAEGGTFARGMENS
                     TSSPKMTDVSVSASGGSTENTGVDNYLHSSPTIRESIISASGTASFGVWGHFPDDTTS
                     TVTIENSEVKGTTGTVKAEGGYKVNLGASKLGGGAVSTIGAGSAVKCAGVHDENYAFS
                     TGPTCP"
     gene            4142..4747
                     /locus_tag="VHM69_19210"
     CDS             4142..4747
                     /locus_tag="VHM69_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742577.1"
                     /translation="MADVGRLSYGQERHLGQAKGEGHDENRKPQSALTKEGLPTGSPV
                     RLTKNAAFDGQAAISPGGKRMAFVSERDGDREIFVMNANAPEGRRNRPVSITNNTVPD
                     QMPDWSPNGEQIVFQRGPDTGPSEIFKMNPNGRGRVNLTNTAALAERDPCFSPDGSKI
                     AFERETSGNREIWRMRAGGRNKVDLTNNPAVDANPSWQLVL"
     gene            complement(4978..5454)
                     /locus_tag="VHM69_19215"
     CDS             complement(4978..5454)
                     /locus_tag="VHM69_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742578.1"
                     /translation="MRRIGIIAVLSLMLVALTAAVAQAGAPKFHSATSSVNNSGALVV
                     SFDERGLGNGNIDYTLTADATATYACINGGGKNPSAANKQDVNGEVTGGGSFESKNGR
                     VSASLSAGPISAGSFTCPNGQRLVLAAVSYTNIVLTDTTNGVSTSVPDASRIFFAV"
     regulatory      complement(5696..5779)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6601..7887)
                     /locus_tag="VHM69_19220"
     CDS             complement(6601..7887)
                     /locus_tag="VHM69_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742579.1"
                     /translation="MSARTASRLAWSLAALAVAMFGAAVVLYVLTPSDNSPGDWFSVR
                     ALSDALGFVAFLAFPLVGALIASRRPDNPIGWICLADGLLWMVLAVTDQYSIYGLASP
                     GSVPFPVAVGTIGNQWLWVSTVGLLGIYLLLLFPNGRLPSSRWRPLAWFAGVVIFVSS
                     IVEGFSPGPLENQGGVPNPFGLEELPWLSEVSLVLLPLLPLCILASAFSLVLRYSRSG
                     GEIRQQIKWVAFIASFTGLLYLIAMVSQLIALQGQMDHSGKLALPLWFEILFSLAVLG
                     FAGVPVAIGFAVLKYRLYDIDLVINRTLVYGSLTATLLALYLAGVSSAQTILRLLTGQ
                     EHQPQLAIVASTLVIAALFNPLRRRIQSFIDRRFYRSNYDARKTLEEFSTRLRKETDL
                     EALSAELVGVVIDTMQPERVSLWLRPEASPKSEEAD"
     gene            8551..9009
                     /locus_tag="VHM69_19225"
     CDS             8551..9009
                     /locus_tag="VHM69_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX2742580.1"
                     /translation="MRKAMLLAAMLAMVLAAAAPAFAQAVGGNVDIQYVDCSQVQAAA
                     AAQGQYGDATADADASAGDFASAADADAAAAIAQELGITQEQVNACLGGIDNDEVSGG
                     GTTTVTVTTTGTSTATAAAELPSTGGTSLLALGAGALLVAGGLLARRIVR"
     gene            9237..>10279
                     /locus_tag="VHM69_19230"
     CDS             9237..>10279
                     /locus_tag="VHM69_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742581.1"
                     /translation="MTEETSAQGRGEDPGGISTRTASWLAWSLCGATVLILALSLLLI
                     LHGWSMQLPNVRPPWRNQAISLIGIIGAPILGGLIASRRPNNPYGWLWLGFGLGLALQ
                     LLAKPYAAHAQLDTGSLPSPQTISHMLSLGGPLALGIAPFLFLLFPTGQLPSRRWRYL
                     AWISALSGAVLLFLTVLFNRPDKVGGPILVIVWIVVSSIFVTIILSALSLVARYRRAN
                     GVERQQLKWFALAAVFAACYIFAQGVGLNRVMGPTLWQLFDSATNMFLYAAVGVAVLK
                     YRLYDIDLVINRTLVYVSLTAMLVALYLAGVSTAQTTFRLLTGQEQQPQLSIVASTLV
                     IAALFNPLRHRIQS"
BASE COUNT         2141 a         3246 c         2985 g         1907 t
ORIGIN      
        1 ccccacagcg aacgtcatag ccaccttctc tgaggcgatg aacgaagcgt cggtggagaa
       61 gaccaccgcc aacgacctac caaccacttt caccctcaag aagaagggca ccacgaagac
      121 gctcgcggcc acggtagtct acagcgagcc cacggcgacg acgttcaagg cgggcctcga
      181 tccgaccagg aagctcaggg cgggtgcgac ctacatcgcc acggtcagtt ctgtagccac
      241 tgacctggtc ggcaacgccc tcgaccagaa cccgagcctc accggcaacc agcccaagac
      301 ctggacgttc actgtgaagc cctagcggcc tgagcaccct tactgcggtg ggctttagca
      361 acggcccacc gcagtagcaa aggagcagaa ctcagagaca gggacgaacg agcgagactg
      421 acgcaggagt aaacaaggag cgaatggaat gacgagtaca agcgtagtaa agaagctccg
      481 ggagcacagg tgggcggctg tcatcttcgc ggcgctcctg ctatccacgc tggcgctagc
      541 gttcgctttg accgcgggac ctgcgcgagc catcgacgac gatccatgtc cgccgatctg
      601 cgaccctccc gaaaacaatc cgccctccgt cgccgcgacc aatccttcca gcacggccaa
      661 cgagggccag acggcgagca acgacggcac cttctcggac gccgacggca acgccactgt
      721 caccctctcc gcctcggtgg gaagtgtgac caagaacctg aacggcacct ggagctggtc
      781 cttcaacacc agcgacgacc cggatcagag ccagaccgtc acaatcaccg ccgacgatgg
      841 tacggagacc gcgacaacca ccttctcgct aaccgtcaac aacgtggccc cgacggtcaa
      901 cctgcaaagt ggcagctttt cgagcgtaca ggagggtggc tcctacgcca atgggtatac
      961 ttcctacggc tataacctct cggtgagcga ccctggcaac gacacacgga ccctgaacac
     1021 aggctgcggc gccaacggct ccgaggggtt cgagagcaca actcaggtct ggtgcggttt
     1081 cttcgacgga ccggggtcgt ctaccgtgag cgcgaccgcg accgatagtg acggcgcctc
     1141 cagaaccgcc agcgtgttag tcaacgtcac taacatgcac ccggttgcgc aggtcgacgc
     1201 cacgacttcg ataaacgagg gcagcacggc gacggtctcc ctcatcaaag gcggcttctc
     1261 gtatcaggac ggcgagaccg acacaaacgc aggcttccgc tacgccttcg actgcaacaa
     1321 cggcccgctc acgacataca actacgccaa cagcggcacc gccaactccg tcaactgccc
     1381 gactacagac aacggcacga agacggtgcg ggcgaagatc atggacaaag acggcggctc
     1441 aaccgagtac acgaagtcca tcacagtgaa taacgtgtct ccaacggcga ccttcaacac
     1501 cccgacctcg gtgaatcagg gcaacaactt cacgatctct ctcggcagcg tctccgatcc
     1561 gtcgagcaat gacaccctca cctacgcctt cgactgcggc gacggctcgg gctacggtcc
     1621 ccccagcagc actccgagca agggcgactg ccccgccctc gacaagccga ccctgacggt
     1681 caaaggcaaa gtcatggacg acgacggagg ctccaacgag tacaccgcca acgtcaccgt
     1741 gaacaacgtc tttccgacag gcacgatcca ggtcaacggc ggagcccccg cgaccaacaa
     1801 tgccgtcgtc aacctcgccc tgtcggccaa tgacccgctg ccaggctcca acgtagatca
     1861 gatgcggttc cgcaacgaga acaccacaac ctggcaggcg tgggaggact acgccaccag
     1921 cagggcgtgg cagttgagca gcggcgacgg gaccaagacg gtcttcgtgg agtacaagga
     1981 caacgctggc aacgcctcgc aaggcgtcat aagcgatcag attttgctcg acaccacgga
     2041 cttcacggct cccacggtcc tgaaatggac accgacgacc aagagagtct cccccacagc
     2101 gaacgtcata gccaccttct cggaggcgat gaacgaagcg tcggtggaga agatcaacgc
     2161 aagcggtctg cctaccacct tcacgctcaa gaagaagggc accacgaaaa cgctcgcggc
     2221 aacggtggtc tacagtgaac ccaccgcgac tacgttcaag gctgttctca acccgagcag
     2281 gaacctcagg gctggggcga cttacattgc cacggtgacc tccgcggcga cggacttcgt
     2341 gggaaatccc ctggtgaaga agacctggac gttcacggtc aagtagcgtg cagaaggagg
     2401 gcctgggatc taaacgcccc tggccctcca ttcaccttgc ctccgaggat gctggtgccg
     2461 tacgctctcg gcagatcgtc gcaactctgg gcttgttcgc cgccccggcc tacgggcagg
     2521 tagggccagt ggagaaggcg tagttctcgt cgtgcacccc ggcgcacttc accgcgctcc
     2581 cggcaccgat ggtggacact gctcctccgc cgagcttgct ggcgcccaga ttgaccttgt
     2641 agccgccttc ggccttgacg gtgccggttg tgcccttcac ctcggagttc tctatcgtca
     2701 ccgtgctggt cgtgtcgtca gggaagtggc cccacacccc gaagctggcc gttccgctgg
     2761 cgctgatgat gctctcccgg atcgtcgggg acgaatggag gtagttgtcc acccctgtat
     2821 tctcggtgct cccaccggaa gcgctgacgc tgacgtcagt catctttggc gaggaagtgc
     2881 tgttctccat gccacgggcg aaggtccccc cttctgcctt tgcgctcacg tcggtcatcg
     2941 tcgggaaaga tccagcgttg tttatgccga cgttgtagtc ggcccccgag ccctttgcgc
     3001 tcacgtcggt catcgtcggg gaggacccgc cggagttggc caccccgaag gcgctgttgc
     3061 ccccggatgc cgtcgcggtc acgtcggtca tcgtcgggga ggagctggcg ttgaccacgc
     3121 ctttgttgtc gaccgcgccc ccggctgtgg ccgtaacgtt cgagagccgg ggcgaagccg
     3181 aagcgttgaa gatcgccata gcatccgtac caccgccagt attctccacg gtcaggtggc
     3241 gcaactcggc gtcgttagcc cccgatacca cgctgctgcc ggcggcgaaa cctgtcccac
     3301 cgggggcggt tatcgtggtc gcgagctccc cggccccctc tatgtccacg aagggcttca
     3361 tctggacccg cccgtcgtag gtccctggtc cgatccacac caggtagtgc ttgctgccgc
     3421 tcgcgtcggt tatgctgtcg agcgccgcct gcacggaggt gaagtcccca cccgacttcg
     3481 ccacaaccac cgtctgggcg tacttggcct ggaggttcgt cgagtccagg ccgtcaatct
     3541 cgtcggcgtt caggttgtca accttcttcg tggagttgac cttcatcggc acgacgtcgt
     3601 ttgccggcac cgtcgggctt cccaccctga gatcgagcgc cgcaccgccc ctgttgttgc
     3661 gaaccaggag cgcggccttg gtcgtgtcgg ctatgctgcc aacgagtcct gtcaccttag
     3721 tcgcagtatt tgcggtcgtg cccaggatga acgaatcacc gttcgctccc agagccgcag
     3781 aagccgcccc gaagatcaag gctagtatca cggagaggcc gacgaggaac acggtggctc
     3841 tgccaaccca catcaccttg ctcgccgtgc tcctgaacat gacgcccaca ctccttgcgc
     3901 tctctcacgt aacgaaggca ccataccccc tgtgcggacg gagcgtatcc cccatttgga
     3961 ctagttctcg ggcctttttg gctgacctcc atgcaaggcc atcgcagtcc cgtgcgcaat
     4021 ccaccagatc ccggcctaac ctcgactccc cgctgaagaa acgtatagtg ccggcgtccc
     4081 aaagagaaca gattctgcgg ggtaatcaga aagttcccac ccgtaacgcg ggccgtaacg
     4141 attggcagac gttggaaggc tatcgtatgg gcaagaacgc cacctggggc aggcgaaggg
     4201 agaaggtcat gacgagaacc gcaaacctca gagcgccctc accaaggaag gacttccgac
     4261 cgggtctccg gtcaggctaa caaagaacgc ggccttcgac ggtcaggcgg ccataagtcc
     4321 cggcggcaaa agaatggcct tcgtttcgga gcgcgacggg gaccgtgaga tcttcgtgat
     4381 gaacgcaaac gccccggagg gccggcgcaa caggccagta agtatcacca acaacactgt
     4441 ccctgaccag atgcctgact ggtcgccgaa cggcgagcag atagtcttcc agagaggccc
     4501 ggacaccggc ccctcggaga tcttcaagat gaaccccaat ggcagaggcc gggtgaacct
     4561 caccaacaca gccgcgctcg ccgaacgcga cccctgcttc tcacccgacg gtagcaagat
     4621 cgccttcgag cgagagacca gcggcaatag agagatctgg aggatgaggg cgggcgggag
     4681 gaacaaagtg gacctcacca acaacccggc agtagacgcc aacccttcct ggcaacttgt
     4741 tctgtaggac tgcccgtgca gtgaagtacg gcgacccgaa cgccagagac agctggcgag
     4801 ggaatgtacc cgaaggggag gctgcgggaa tctgtacgcc ggggacatgg aactgccagc
     4861 ctcgttctgg tctccccggg cacggaatac gctgagggcc ggaacttttg agttccggcc
     4921 ctctgaccga aatcggcgct cctttagaga agccaccaac ttccactatc cttcggccta
     4981 gacggcgaag aatatccttg acgcatcagg cacgctggtc gagacgccgt tagtcgtgtc
     5041 cgtcagcacg atgttcgtgt acgacacggc cgcaagtacc agtcgctgac cgtttgggca
     5101 ggtgaaacta cccgcggaga tcggtccggc cgagaggctg gccgagacac gcccgttctt
     5161 ggactcgaag gagccgccgc cggtcacctc accgtttaca tcctgcttgt tggcagccga
     5221 agggttcttc ccacctccgt tgatgcacgc atatgtggcc gtcgcatcgg ctgtgagcgt
     5281 atagtctatg ttcccgttac ccagtccgcg ctcgtcgaag cttacgacga gggcgccact
     5341 attgttgacg ctcgacgtcg cgctgtggaa cttcggcgcg ccagcttgcg ctacagcagc
     5401 cgttaaggcc accagcatca aggacagtac ggcgattatg ccgattctcc tcattaagga
     5461 ctctccttcg ttcccggcct ctactcctct tttcagggac agggctattt acggtcccac
     5521 cttagagcta gtagaatgcc tggttgcggg gtcgggcgtt cttgtatcgg tgcccagtcg
     5581 aatgacaagc gtccggcccc ttccactttg acttcttcgt tagctaacct cgcgcaatca
     5641 cctcctctat tgcataattc cattccccaa aggaaaaccg gccattggat cattgatcca
     5701 gagtagccgg ttgcaccact ggctccctct gccccaggtg cccatcttgg gggcgaagtt
     5761 ctgagcctct acgtagccct attttcccgc gcacacgatt ctagcaccag ggcacggtgg
     5821 cttcgtatgc cgatcggcgt atttttacgg taacttgcgg gttacatgtt gtggccagga
     5881 gactcagcag actttctgcg tatccacgaa tgtgtcgggg tcaagcgctc aacaggccgg
     5941 attcgatccc gcgagcactt gctcgtcacg gcggcaaccg accctgggcg tctcgggtag
     6001 acttcgtact cctcatgcga ggccccgact ccggttgcag gcgtgccgcc taagccacga
     6061 ccgagatctc atctcctggg tctcgaagac acactcctga tgcacacctt gggtctgtca
     6121 gcggcttgcg gaaggaatgc tcccacaagc cgggcaacag gtttcaggca tcaggaaggt
     6181 tttcccggac ggacggtggt cgctaaggtg cacctcttgg gcgaggtgga tcaatactta
     6241 tgtactgcgc tgtgagatga ttactgactg ccccaaggga accggattgg cacgggagtc
     6301 cggccgggac ggagggggga tgccccgacc agactacgct accaccgaga acgtaacacc
     6361 ccggtcttta agcgttgtta acgacagagg agggaccgtt aagattcacc ccgatacgat
     6421 cagacatatg ctttgatgcg ctgatggagg gggtgacggg tggtcagcag gttgaccacc
     6481 cgtcgttggc ctactcatgt actacgcaac gcgagagatt cgcgtgaaca tagaagtaat
     6541 gttggccggt gcgaatctac gggaagatcc cttccaggag ccttgatgca cagggtactg
     6601 ctaatccgcc tcctcgctct tcggtgacgc ctctggacgt agccacaaag agacacgctc
     6661 gggctgcatg gtgtcgatga ccacccccac aagctctgca ctcaatgcct caagatcggt
     6721 ctccttacgc aatcgggtcg agaactcctc aagtgtcttc ctcgcatcgt agttgcttct
     6781 gtagaaacgc ctatcgatga aggactggat gcgacgcctc aaggggttga acagcgctgc
     6841 tatcacaagg gttgaggcga cgatagcgag ttggggttgg tgctcctgac cggtgaggag
     6901 acggagaatg gtctgagcag agctgacgcc agcgaggtag agagcgagaa gggtagctgt
     6961 gagcgaacca tatacgagag tacggttgat gacgaggtct atatcgtaga ggcggtactt
     7021 caggacggcg aagccgatag ccactggcac gccagcgaag ccaagtacgg cgagcgagaa
     7081 gaggatctcg aaccacaacg gcagagcaag tttaccgctg tggtccatct gcccctgcaa
     7141 ggcgatcaac tggctaacca tcgctatcaa gtacagcaag ccggtgaacg aggcgataaa
     7201 tgcaacccac tttatctgct gacggatctc tcccccggaa cgactgtaac gcaacaccag
     7261 gctaaaggct gaagccagga tgcacagtgg caacaacggg aggaggacca gcgatacttc
     7321 agacaaccag gggagttcct cgagtccaaa ggggttgggt acgcctccct ggttctccaa
     7381 cggtccggga gaaaacccct caacgatgct ggacacgaag atcaccaccc cggcgaacca
     7441 tgccagcgga cgccatctgc tcgaaggcaa cctaccgttc gggaagagca ggagcaggta
     7501 gatcccgagc agccccaccg ttgataccca cagccactgg ttgcctatcg tgccgacagc
     7561 cacagggaaa gggaccgaac ccggactggc gaggccgtag atgctgtact ggtcggtaac
     7621 cgcgaggacc atccacaaga ggccgtcagc aaggcagatc caacctatcg ggttgtcggg
     7681 acgcctggaa gcgatcaggg cgccaacgag ggggaaggca aggaaggcca cgaagcccaa
     7741 cgcgtcgctc agggcgcgga cgctgaacca gtcaccgggt gagttgtcgg acggagtgag
     7801 gacgtagagc acgacagccg ctccgaacat ggccacggct agcgcggcca gcgaccacgc
     7861 tagccgggag gccgtacggg cactcatccc acctcttcct cgaccctgtt ctgttttgtc
     7921 acgacaccac tctacagtac gcccgtctcc taaaggttac ggcacccgtt acgagagagc
     7981 agatcgcaat tgcacgtcgg tgaatctggc ttgctcacgg tcctggcata cttatgactg
     8041 aatcatcgtc gatagcctgc ccgccttttg tacggacggc aaaaggatag ccttcgacag
     8101 tggccgtggg atctggcaga tgcactcaga cggcagccac atgatgaacc tgaccgacaa
     8161 catagacatc gtcgacgcta acccctcctg cagctagacg atgttcgcgc ccttctctgc
     8221 gaagcccaga aaaggctcgc cacacatttc gtgaacaaag tacgagaacg ccatcctggc
     8281 gacgatgcgg gtctcccgag ccgtctcttg tcaagctggt gtcttccgcg aggttggcga
     8341 ggcctaaatc cgcaccatgt ggtggttgcg cacgtcatgg cctctatatg tagttttagt
     8401 tgcaaggaat atgaagaaat gttaagatac atttggaaag ttcctccggg cgggcatagg
     8461 caagaaggtg aacggggagg tgagccaggt cgagaagaag gtccagcatc ggtggagcgc
     8521 acgaggacta tttacgggga ggtaatgaag atgaggaaag caatgttgtt ggcggcaatg
     8581 ctggctatgg tgctggctgc tgctgccccg gctttcgccc aggcggttgg tgggaacgtt
     8641 gacatacagt acgtcgactg ttctcaggtt caggcagcgg ctgctgcgca gggtcagtat
     8701 ggcgacgcca ctgctgacgc tgacgcttcg gctggcgact tcgccagcgc tgctgacgcc
     8761 gatgcggctg ccgcgatcgc ccaggagctc ggtatcaccc aggagcaggt caacgcctgc
     8821 ctcggtggca tcgacaacga cgaggtcagc ggtggcggca ccactacggt gaccgttacc
     8881 accactggca cgagcaccgc tactgcggct gccgagcttc ccagcaccgg tggcacttcg
     8941 ctgctcgcgc tcggtgctgg cgccctgctt gttgccggtg gacttctggc acgcaggatc
     9001 gttcggtaag taaccggaca cgttaggcat catgaggcgg gtcccttcgg ggcccgctta
     9061 tttcgtgcgc gcctttcttc agcaccatcg gcacgccggt gatgcgtccc ttttccagtc
     9121 actatcttgc cgaatatctc gacggggtag tgagttctca agagcttgcc gtgaacgact
     9181 ttgctcgtaa cgggtgccgt aacgtctggg agacggacat catgtagagt ggtgtcgtga
     9241 ccgaagagac aagcgctcaa ggccgaggag aagaccccgg cgggataagc actcgaacgg
     9301 cctcatggct ggcgtggtcg ctgtgcggcg caaccgtgtt gatcctggcg ttgtcgctgt
     9361 tgctgatcct ccacggctgg tcgatgcagt tgccaaatgt gcggccaccg tggcggaatc
     9421 aggccatctc cctgatcggg ataatcggtg cgcccatcct cggtggcctc atcgcctcgc
     9481 gccgcccaaa taacccttac ggatggctgt ggctcggatt cgggctgggc ctcgcccttc
     9541 agctgcttgc aaagccctat gccgcccacg cgcagctgga tacggggtca ctaccctctc
     9601 cacagacgat ctcccacatg ttgagtctgg gagggccact cgcgctcggc atcgcgccct
     9661 tcctcttcct gctgttccct acgggccagc tgccgtcgcg acgctggcgc tacctggcct
     9721 ggatctcagc cctttctggg gctgtgctcc tgttcctcac cgtcctcttc aataggccgg
     9781 acaaagtcgg gggaccgatc ttggtcatag tatggatcgt ggtttcttcg atcttcgtga
     9841 cgatcatcct atcggcactc tcgctcgtgg cacgatatcg cagggcgaac ggagtcgagc
     9901 gccagcagct gaaatggttc gcgttggccg ctgtcttcgc cgcatgctat atcttcgcgc
     9961 aaggcgttgg cctcaaccgg gtcatgggtc ccactctgtg gcaactgttc gactctgcaa
    10021 ccaacatgtt cctctacgcg gccgtcggtg tggccgtcct gaagtaccgc ctctacgata
    10081 tagacctcgt catcaaccgt actctcgtct acgtctcact cacagccatg cttgtcgccc
    10141 tctacctcgc tggagtcagc actgctcaga ccacattccg tctcctcacc ggtcaggagc
    10201 agcaacccca actatccatc gtcgcctcca ccctcgtcat cgccgccctc ttcaacccct
    10261 tgaggcaccg catccagtc
//
LOCUS       DATAIR010000809         5809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_230386, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000809 DATAIR010000000
VERSION     DATAIR010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230386"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VHM69_19235"
     CDS             complement(<1..577)
                     /locus_tag="VHM69_19235"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HEX2742582.1"
                     /translation="MKISDIRTICTAPEGIRLVVVKVETDEPGLYGLGCATFTQRPLA
                     VVTAVEEYLKPFLIGRDPDEIEDIYQSAYVSSYWRGGPVLNNALSGVDMALWDIKGKR
                     AGMPVYQLFGGRCRNAVSLYAHATGSNFSEVEDEARRYMEEGYRYVRCQISVPGYSTY
                     GSTISAGPQPLGLGDDPWEPTPYCRLIPRLFE"
     gene            complement(574..1443)
                     /locus_tag="VHM69_19240"
     CDS             complement(574..1443)
                     /locus_tag="VHM69_19240"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2742583.1"
                     /translation="MQEIEGVYVANVTPFKDDGSLAVDDGAYVDHVSWLAEMGVRGVV
                     PFGTNGEGPSVTLGEKLRVLEALFERGLRTQIIPSVMQNNLPETLEMLGALEDFPATA
                     VLVLPPYYFKPVAPEGMVRFYEPVLAATSHPVIVYHIPKYAVPVPEEVVIGLPVWGVK
                     DSGGEEGYAEAVLAGGKGVLLGTEDDLFRRLDTGAPGVISALANFMPEMVLELYRKHK
                     EGDEAGGKALSEKLQKARAMTKEYASPAVLKKLAGARHGASMGTVRPPFVPVPADYDP
                     EAALESVRTRAGN"
     gene            complement(1489..2439)
                     /locus_tag="VHM69_19245"
     CDS             complement(1489..2439)
                     /locus_tag="VHM69_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX2742584.1"
                     /translation="MSGNLDVVTAGETMVLGVPPRPGRLRHAGSLELKIGGAESNLAI
                     ALSRLGLSAGWVSYIGDDEPGQLVLDRVRAEGVDTSRVRRMKDHPTALYLREQVGKDV
                     RVYYYRKGSAASTMRPGAFDPDHLSGAKFLHLTGITPALSEDCRAFTLWAAREARSSG
                     VRVSFDVNYRSKVWETETAREFVEEILPDVYLLFVGDEEAMALWARDDEGLVRELAHA
                     GPEEVVLKRGGAGSLALVEGEILEDPAFSVTQVDPVGAGDAFAAGYLAGHVWDMPARE
                     RLRVANAMGAMSVATLGDYEGLPDEKELRAFLGGEKSLGR"
     gene            2534..3370
                     /locus_tag="VHM69_19250"
     CDS             2534..3370
                     /locus_tag="VHM69_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2742585.1"
                     /translation="MVEIELIGRIDALEVGERALAFWGLGQVGLVIKGPTGIVYVDPY
                     LTDSDGAGGSLPRTFPPPLVPGEVTNASAVLLTHDHIDHTDPQTVLPISEASPEARFV
                     APFTSRDTLDGAGLDAERINVPAVGEPVEVAGASVTAIPSAHTELEHDRERGYPYLGY
                     VIEWNGVTVYHAGDTVVYEGMIETLSAWDIDIAFVPINGRDYFRTGRGIIGNTDFRET
                     AELAETLNVGLIVPTHYDLIEGNTADPGHFVSHLYGLNPMRPHKLLRPGELLYFVKGP
                     SD"
     gene            3504..4319
                     /locus_tag="VHM69_19255"
     CDS             3504..4319
                     /locus_tag="VHM69_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2742586.1"
                     /translation="MYLSRHQAAGGPRWALDGRYLPQDFTLERLLGMSATEARSFLEG
                     QSREEEAGESLLPPVEPAHEVWASGVTYLRSREAREFESADADAYERVYDAERPELFF
                     KAQGWRVVGHGDRVRVRVDSHWNVPEPELVLVVNSGMEIIGYTAGNDVSSRDIEGENP
                     LYLPQAKVYDGSCSLGPGIVLSGPDSMRDLRITMSIRRGGEIVFEDETGTSQMKRSPE
                     ELASYLGRELALPSGAFLMTGTGIVPEDEFSLEPGDLVGISVGDLMLENEVTR"
     gene            4386..>5809
                     /locus_tag="VHM69_19260"
     CDS             4386..>5809
                     /locus_tag="VHM69_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2742587.1"
                     /translation="MIESQIESLNFIDGEWVPADSGETSEIVNPSNPSEVLGTTPKSD
                     KAETERAIEAASEALPGWKATLPAARGAILMEAANIIESREDELARLMALEAGKPMKE
                     ARMEVGRAASIFRYYGSEGWRMPGIAPPSARPGVSHSSIREPLGVVGLITPWNFPLAI
                     PSWKMAPALICGNTIVMKPASNASLNAAALVRILAEAGLPAGVLNMVTGPGGSVGDAL
                     VTDSRVKGLSFTGSTGVGLGIQSRAVGKKVQLEMGGKNPYVVMEDADLSDAAAKVSFG
                     AFGYAGEKCTASSRAIVVEEVYDEFIEKLKSATEAMKVGDPLEEDTDIGPVVNKSQYE
                     SILEALETAKTEGRVVLEGGATGSEDDGYYIAPAIFADVDNKSETAQEEIFGPVLAII
                     KARDFDDAVDLANDTRFGLTAGIATKSLRYAHEFTQRSETGLVNVNLPTAGLEFQVPF
                     GGNKESGVGGREQGPAALDFYSAW"
BASE COUNT         1111 a         1891 c         1803 g         1004 t
ORIGIN      
        1 gctcgaagag gcgggggatc aatcggcagt aaggcgtcgg ctcccacggg tcgtctccca
       61 gtccaagcgg ctgcggtccg gctgagatcg ttgacccgta agtagagtag ccgggaacgg
      121 agatctggca ccgcacgtac ctgtaaccct cctccatgta cctgcgcgcc tcgtcctcga
      181 cctcgctgaa gttgctgccc gtggcatggg catagaggga aaccgcgttc cggcacctgc
      241 ccccgaagag ctggtagacg ggcataccgg cccgcttgcc ctttatgtcc cacagcgcca
      301 tgtccacgcc cgagagggcg ttgttcagga cgggaccgcc gcgccagtac gagctcacgt
      361 aggctgactg gtagatgtcc tctatctcgt cggggtcgcg tccaatcagg aacggtttca
      421 gatactcctc gacggccgtg acgaccgcga gcgggcgctg ggtgaaggtg gcgcacccga
      481 ggccgtagag tccaggctcg tccgtctcga ccttcacaac cacgagcctg atgccctcgg
      541 gtgcggtgca gatggtcctt atgtcggaga tcttcaattg cctgccctcg tacggacgct
      601 ttcaagcgcc gcttcggggt cgtagtccgc cgggacgggc acgaagggcg gtcggacggt
      661 ccccatcgac gccccgtgcc ttgcccccgc gagctttttg aggaccgcgg gagaggcgta
      721 ttccttggtc attgcccgcg ccttctggag cttctcagag agggctttgc ctcctgcctc
      781 gtcgccttcc ttatgcttgc ggtacagctc gagaaccatc tctggcatga agttggcgag
      841 ggcggagatc acacccggag cacccgtatc gagcctgcga aagaggtcgt cctccgtgcc
      901 gagaagcacg cccttgcccc ccgcgagaac ggcttcggcg tagccttcct ccccgcctga
      961 atccttgacg ccccatactg gaagtcctat gacgacctcc tcagggacgg ggacggcgta
     1021 cttcgggatg tgatacacga tgacgggatg gctcgtcgcc gcgagcacag gctcgtagaa
     1081 gcgcaccatt ccttctggtg cgactggttt gaagtagtaa ggcggcagga cgagcacggc
     1141 ggtagcagga aaatcttcca gagcgccaag catctccagc gtctctggca ggttgttctg
     1201 catgacggag gggatgatct gtgtccgtag cccgcgctcg aagagggctt cgaggacacg
     1261 cagcttctca ccgagcgtca cagaaggccc ctctccgttg gtgccgaagg gcacgacgcc
     1321 acgcacgccc atctccgcga gccaggagac gtggtcaacg tacgccccat catcgaccgc
     1381 cagagagcca tcgtctttga acggcgtcac gttcgctacg tagacgccct ctatctcctg
     1441 catcctctta cctcccttaa ggacttcaag ttaccacgcc cgaactgctc accttcccag
     1501 agacttctca ccaccgagga aggcgcgcaa ctccttctcg tcgggaaggc cctcgtagtc
     1561 cccgagggtc gcgacgctca tggcgcccat cgcgttcgcc acccggagac gttccctggc
     1621 gggcatgtcc cacacatgac cggccaggta tcccgcggcg aaggcgtcgc cggctcccac
     1681 ggggtcgacc tgcgtcaccg agaaggcggg gtcttccaga atttcaccct cgaccagcgc
     1741 caggcttccc gctccgcctc ttttcagaac aacctcttcc ggtcctgcgt gcgccagctc
     1801 ccgcaccaac ccctcatcgt cgcgggccca gagcgccata gcctcctcgt cccccacgaa
     1861 gagcaggtag acgtcaggca ggatctcctc gacgaactct ctcgccgtct cggtttccca
     1921 gaccttcgag cgatagttga cgtcgaagct gacgcgtact ccggatgagc gggcctcccg
     1981 cgcagcccac agagtgaagg cgcgacagtc ttcggagagg gcaggcgtga tgcccgtcag
     2041 gtgcaggaac ttcgcgcctg agaggtgatc cgggtcgaat gcacccggtc gcatcgttga
     2101 ggcggctgac cccttacggt agtaatagac tcgaacgtcc ttgccgacct gctcgcgaag
     2161 gtaaagtgct gtcgggtgat ccttcatcct gcgtacccga gaggtgtcca ccccttcggc
     2221 gcgtacgcgg tccagaacta gctgccctgg ctcgtcgtcg ccgatgtagc tcacccatcc
     2281 ggcggagaga ccgaggcggg agagtgcgat ggcgaggttc gactcagcgc ccccgatctt
     2341 gagctcgagg ctaccggcgt ggcgcagacg ccctggccgc ggcgggacgc cgagcaccat
     2401 cgtctctccc gcggtgacta cgtcgaggtt ccctgacacc tgcgacccct cctctacttc
     2461 gcctctcccg aacattatct tacctggagc gcggccctgc ttgtgctaac ctggaaaggt
     2521 tattcaaggg gaggtggtcg agatcgaact gatcggcagg atcgacgcgc tcgaggtagg
     2581 agaacgcgcc ctcgccttct gggggctcgg acaggtggga ttagtgatca aaggtccgac
     2641 cggcatcgtc tacgtcgacc cttaccttac agactctgac ggtgcaggcg gcagcctgcc
     2701 ccgaaccttc cctccaccgc ttgtccccgg tgaggtgacc aatgcctccg ccgtgctgct
     2761 gacccacgac cacatcgacc acacagaccc acagaccgta ctaccaatct ccgaggcatc
     2821 gcccgaggcc cgcttcgtcg cgcctttcac gagccgcgac acgctcgacg gggcggggct
     2881 cgatgcggag cgcataaacg tacccgcggt cggagagcct gtggaagtcg cgggagccag
     2941 cgtcacggcc atcccatcgg cccatacgga gttggagcac gaccgggaac gcggttatcc
     3001 ctatctgggg tacgtgatcg agtggaacgg cgtcaccgtc tatcacgcgg gcgacaccgt
     3061 ggtctacgaa ggcatgatcg agacgctctc ggcctgggat atagacatcg ccttcgtgcc
     3121 catcaacggc cgcgactact tcagaacagg caggggcatc atcggtaata ccgatttccg
     3181 cgaaaccgcg gaactggcgg agactctgaa cgtcggtctt atcgtgccga cgcactacga
     3241 cctgatcgaa ggcaacaccg ccgacccggg acacttcgtc agccatctct acggcctgaa
     3301 cccgatgcgc ccgcacaagc tgcttcgtcc tggtgagctc ctctacttcg tcaaaggtcc
     3361 aagcgactag accggccctt gacggaccag atggtcgaag tgtgaaagag gcggcgctcc
     3421 cttcaccact cacctggaag cctcgcgcaa cggaaccccg atcatggtag gttgtacgta
     3481 tccaggaagg aggctccacc accatgtacc tctcccgcca ccaggccgcc ggaggcccac
     3541 gctgggcctt ggacgggcgc tatctcccgc aggatttcac gctggagcgg ctgctcggaa
     3601 tgagtgccac ggaggcccgc agtttcctcg aaggtcagtc gcgtgaagag gaggccggag
     3661 agtccctact gcccccggtc gagccagctc acgaggtatg ggccagcggg gtgacgtacc
     3721 tgcgcagcag ggaggcccgc gagttcgagt cggctgacgc cgacgcctac gagagggtat
     3781 acgacgccga gcggcccgaa ctcttcttca aagctcaagg ctggcgggtc gtgggacacg
     3841 gcgacagggt gcgcgtccgc gtggacagcc actggaacgt gcccgagccg gagctcgtgc
     3901 tcgtcgtcaa cagtggaatg gagatcatcg gctacaccgc tggcaacgac gtctcttcgc
     3961 gggacataga gggtgagaac ccgctgtatc tcccgcaggc caaggtctac gacggctcat
     4021 gctctctagg tccaggcatc gttctctccg gccccgactc gatgcgggac ctccgcataa
     4081 ccatgagcat caggcgtgga ggtgagatcg tcttcgagga cgagaccggt acgtcacaga
     4141 tgaaacgctc tcctgaggag cttgcaagct atctgggtag agagctcgcc ttaccttccg
     4201 gagccttcct gatgacgggc acggggatag tccccgagga cgaattctcg ctagagcccg
     4261 gggacctggt gggtatatcg gtcggagatc tcatgctgga gaacgaggtg accaggtagc
     4321 acggttcata gaatgaccaa gaatcgggta ggaagggagc aagcgttctt gctaggagga
     4381 agaccatgat cgaatcccag atagagtccc tgaacttcat cgacggcgag tgggtccccg
     4441 cggactcggg cgagacctcg gagatcgtga acccatcgaa cccctcggag gtactcggca
     4501 ccaccccgaa gtcggacaag gcggaaacgg aacgggccat cgaggccgca tctgaagccc
     4561 tgcccggttg gaaggccact ctccccgcgg cccgcggcgc aatcttgatg gaggccgcga
     4621 acatcataga gtctcgagag gacgagctgg cgcggctgat ggcgctcgag gcgggcaagc
     4681 cgatgaagga agcgcgcatg gaggtcgggc gcgcggcgag catcttccgc tactacggct
     4741 ccgagggttg gcgcatgcca ggcatcgccc cgccctccgc gcggcccggc gtgagccaca
     4801 gctcgataag agaaccactc ggggtcgtcg gcctcataac gccgtggaac ttcccgcttg
     4861 ccataccttc ttggaagatg gcgccggccc tgatctgcgg caacaccatc gtcatgaagc
     4921 cggcgtccaa cgcctccctg aacgcagcag ctctagtgcg aatcctggcc gaagcgggcc
     4981 tccccgccgg ggtcctcaac atggtgacgg gacccggagg ctcggtcggg gacgctctcg
     5041 ttacggactc gcgcgtgaag ggcctctcgt tcacgggctc gacgggcgtt ggcctcggca
     5101 tccagagccg ggccgtaggc aagaaggtcc agctcgagat gggcggcaag aacccatacg
     5161 tcgttatgga ggacgcggac ctctccgacg ccgccgccaa ggtctccttc ggcgccttcg
     5221 gatacgccgg cgagaagtgc acggcctcaa gccgcgccat cgtcgtcgag gaggtctacg
     5281 acgagttcat cgagaagctc aagagcgcaa cagaagccat gaaggtcggc gacccgctcg
     5341 aagaggacac agatataggc cccgtcgtca acaagagcca gtacgagagt attctggaag
     5401 cgctcgagac ggcgaagacc gaagggcgag tggtcctcga aggcggcgcc acgggttctg
     5461 aagacgatgg ctactacatc gcccccgcaa tcttcgccga tgtggacaac aagagcgaga
     5521 cggcgcagga ggagatcttc ggccccgtcc tcgccatcat aaaggcccga gacttcgacg
     5581 atgcggtcga tctcgccaac gacacccgct tcggcctgac cgctggcata gcgacgaagt
     5641 ccctgcgcta cgcccacgag ttcacacagc gctctgagac gggcctcgtg aacgtcaacc
     5701 tgcctacggc cggcctggag ttccaggtac ctttcggagg caacaaggaa tctggagtcg
     5761 gcggacggga gcagggaccg gcggccctgg acttctactc ggcctggaa
//
LOCUS       DATAIR010000810         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_367929, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000810 DATAIR010000000
VERSION     DATAIR010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367929"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..563
                     /locus_tag="VHM69_19265"
     CDS             150..563
                     /locus_tag="VHM69_19265"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX2742588.1"
                     /translation="MIEQAKEGWRHWRTFKEIDAGHRYQSRYHFHQRRRRRGEISRYR
                     RPINLIAGPILIIAGFVFIPTPGPSYIIIALGLWMLAGEFLPLARLFDRMGVRLGRLW
                     RWIKGVWRTSSVVLRGQIIALFLVCIAALLYWLIY"
     gene            complement(570..>1759)
                     /locus_tag="VHM69_19270"
     CDS             complement(570..>1759)
                     /locus_tag="VHM69_19270"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEX2742589.1"
                     /translation="TLLSCHDASEVPLTRKILDRPRVVERIRAAIADPEAAHLTCFNS
                     TPLERSLAVRLGIPLYGNDPELNYLGTKSGSREAFREAEVPLPEGFEHLRDADDVIEA
                     VVELKRRNPGLRRAAIKLDEGFSGEGNALFAYDGAPEGRDLARWVRDELPRRIRIESV
                     GETWERYQRKFEEMGGIVECWMEGEEVRSPSVQCRINALRQETVVSTHDQVLGGPSGQ
                     IFLGSTFPADAEYSREIKDVGERVGKVLRERGAMGRFAIDFISVRRGDAWEHAAIEIN
                     LRKGGTTHPYLILRFLTDGVYDPNDGLYCTPTAKPCFYYASDNLQSPTYKGLTPDDLV
                     DIAVDNGLHFDGASQQGVVFHLIGALSQFGKVGTVCIGDSHESARKYYRDTVAVLDRE
                     AQR"
BASE COUNT          289 a          563 c          535 g          372 t
ORIGIN      
        1 gtctggaaga gttccgtcct gggcaaggtc gctgtcgtag ttgtagcctt gattctagcc
       61 gccgccatcg tctacgtcgt ttatctcgtg ctcatcagcg tatcgttgtt ctgagtaagc
      121 ccgtgaccct atggaaacgc actctgggcg tgatcgagca agcgaaggag ggctggaggc
      181 actggcgtac tttcaaggag atcgacgccg gccaccgtta ccagagccgc taccactttc
      241 accagcgccg ccgcaggcgc ggtgagattt ccaggtaccg cagacccatc aacctcattg
      301 ccggacctat tctgataatc gccggcttcg tgttcatccc cacgccgggc ccgagctaca
      361 taatcattgc tctcgggctc tggatgctcg ctggcgagtt cctgcccctc gcccgtctct
      421 tcgaccggat gggtgtgcga ctcggaaggc tctggcgttg gatcaaaggc gtgtggagaa
      481 catcgtccgt cgtgctgagg ggacagatca tcgcactgtt cctagtctgc atcgccgcgc
      541 ttctctactg gctcatctac tgatccgtac taccgttgcg cctcgcggtc caggaccgcg
      601 accgtatcgc ggtagtactt cctggcgctc tcgtggctgt cgccgataca cacggtgccg
      661 accttgccaa attgggagag cgccccgatg aggtggaaga cgaccccctg ctgggaagcg
      721 ccgtcgaagt gcaggccgtt gtccaccgct atgtccacga ggtcgtcagg ggtaagtccc
      781 ttgtaggttg ggctctggag gttgtcagag gcgtagtaga agcagggctt cgccgttggc
      841 gtgcagtaca ggccgtcatt agggtcgtag actccgtcgg tcaggaaacg caggatcagg
      901 taagggtgcg tggtgccgcc cttacgaagg ttgatctcga tcgcagcgtg ttcccaggcg
      961 tccccgcgtc tgacggagat gaagtcgatg gcgaaccttc ccatggcccc ccgctccctg
     1021 agcactttgc cgaccctttc gccgacatcc ttgatttccc tgctgtattc ggcatctgcg
     1081 gggaacgtgc tgccgaggaa gatctgaccc gaaggacctc ccaacacctg gtcgtgggtc
     1141 gagacgacgg tctcctgcct cagggcgttg atacggcact gcacggaagg agagcgcacc
     1201 tcctcaccct ccatccagca ctcgacgatg ccgcccatct cctcgaactt tctctggtaa
     1261 cgctcccagg tctcgcccac ggattcgatc ctgatgcgcc tcggcagctc gtccctgacc
     1321 cagcgtgcga ggtcccttcc ctcgggcgcg ccgtcgtatg cgaagagtgc gttcccctcg
     1381 cccgagaaac cctcgtcgag tttgatcgcc gcgcgccgca gtccagggtt acgccgtttg
     1441 agctcgacga cggcctcgat cacgtcgtcc gcgtcgcgca gatgctcgaa gccctcaggc
     1501 agcgggacct cggcctccct gaacgcctcc ctgctgccgc tcttggtccc gaggtagttg
     1561 agttcagggt cgttgccgta gagcgggatg cccagccgca cggcgaggct gcgctccagc
     1621 ggcgtgctat tgaagcaggt caggtgcgcc gcttccgggt ctgcgatcgc agcccgtatt
     1681 cgctccacca cgcgtgggcg gtccaggatc ttgcgggtca gtggaacttc tgaggcgtcg
     1741 tggcacgaga gaagcgtca
//
LOCUS       DATAIR010000811         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_230457, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000811 DATAIR010000000
VERSION     DATAIR010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230457"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1549
                     /locus_tag="VHM69_19275"
     CDS             71..1549
                     /locus_tag="VHM69_19275"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX2742590.1"
                     /translation="MGSIESQDAGILSVDVGSSAVRAGLYDESGNGVKGTEVKLDYEF
                     RHSQDGGATTDADELFDLVVRAIDGALSRVGKASISGVAMSTFWHSVLGLDREGHPTT
                     PILTWADRRAADSARTLRERLDETAYHRRTGCVAHSSYWPSKLLWMSRTMPEAFERTE
                     HWVSPADYVYGRFFGEPYQVGTSMASGTGLLDQNRMCWDKETLDALPAEEAQLSSISD
                     EPRRGLAGEWAKRWPGLRDTPWLPAVGDGACSNVGSGCTGSDRLALMVGTSGAMRVLW
                     KAETVEIPDGPWCYRADARRFVMGGALSDGGNLIEWLRNTLRLPDTEKTEALLAQMEP
                     DAHGLTFLPLLAGERGPGWADRANGTVAGLSMSSEPVEILRAAMEAVAFRFAIIAEII
                     ETASPGEKEVVASGGGLLNSPTWTRIIADTLGRPVTLSGVDEASSRGAALIAREALGG
                     PEIEEAPSPLGETFEPDPGRHEVYRNALERQRQLYEAVLDRG"
     gene            complement(1662..3074)
                     /locus_tag="VHM69_19280"
     CDS             complement(1662..3074)
                     /locus_tag="VHM69_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742591.1"
                     /translation="MGGKHKGPFARVVAIIVVLAGLSVIGACGADTVATKDQSSRDAA
                     VGSKAAEGEGGSKTIPQPVMLEDLATAPEGARVDISMPVFSNPTEITNPLFPVSKQES
                     VLMVGRVDDKPFRTEVTLLPETRIIEWQGQRVETLVSQYTAYLDGRIHEIAYDLYAQA
                     DDGSVWYFGEDVFNFEDGAIANTEGTWIAGKDGPAAMIMPSEPKMGDVYRPENIPGFV
                     FEEVTVKSVGKTLEGPMGPVDGGLVVEELHMDNTKEGKTFAPGYGEFYTAGGGDVEAL
                     ALAIPTDALSGPTPAELDTLSSGALEVFDAAESEDWEGATTTVEEMNAAWKDYREGEV
                     PKKIEPRMSAALADLTEAVEARDTKQTSQAAIDAAQWSLDLRLQFRPQAEIDLARFDL
                     WAAQLTLDAAAGDANAVRGDVFTLGYIRDRIMSSLDGADATRVNTAFMDLQVAAGEDD
                     LAAASDAAEQLRNTLGQLRN"
     gene            complement(3150..3470)
                     /locus_tag="VHM69_19285"
     CDS             complement(3150..3470)
                     /locus_tag="VHM69_19285"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX2742592.1"
                     /translation="MDKKKLAASAVAVVLALGAGGAAYAVGQGEKVTGPDADKAKSAA
                     LRAVGGGTVTEVERSDGDGAFEVEVERSDGSQVEVHLDRTLDVVGRSAGDDGPNDSEG
                     PNED"
BASE COUNT          595 a         1116 c         1138 g          667 t
ORIGIN      
        1 gatggtaaac atgtaaccgg gtagcgcttg ctaaactacc tgaagggtgg tctgtgtgga
       61 aggtgggagc gtgggatcca ttgaatcgca agatgcaggg attctctctg tagacgtagg
      121 gtcctcggcg gtgagagccg ggctgtacga tgaatcgggc aacggcgtga agggcacgga
      181 ggtcaagctc gactacgagt tccggcactc ccaggacggc ggcgcgacca cagatgccga
      241 cgagttgttc gacctcgtgg tccgtgccat tgacggcgcg ctttcccgcg tgggaaaggc
      301 tagtatctcg ggcgtcgcca tgagcacctt ctggcactcg gtcctcggcc tcgacaggga
      361 aggccatccc acgaccccga tcctgacctg ggccgaccgc cgcgctgcag actccgcacg
      421 caccttgcgt gagcgcctgg acgagaccgc ctatcaccgt cgaacgggtt gtgtggcgca
      481 ttccagctac tggccgagca agctcctgtg gatgagccgt actatgccag aggccttcga
      541 gaggaccgag cactgggtct caccagccga ctacgtctat ggccgcttct tcggggaacc
      601 gtatcaagta ggtacgtcaa tggcctcagg gaccggcctt ctggaccaga acaggatgtg
      661 ctgggacaag gagaccttgg atgccctgcc cgcggaagag gcgcagcttt catcgatctc
      721 tgacgaaccc cgacgcggat tggccggtga gtgggcgaag cgctggcctg gcctgaggga
      781 cacgccatgg ctccctgccg tcggggacgg cgcctgctcg aacgtgggga gcgggtgcac
      841 gggaagcgac cggctggcgc tcatggtcgg gactagcggc gcgatgcgcg tgctctggaa
      901 ggcggagacc gtcgagatac ccgacggtcc ctggtgctac agggccgacg ctcggcggtt
      961 cgtcatgggc ggggcgctct cagacggggg gaacctgatc gaatggctgc gcaacacgct
     1021 acgcctgccc gacacggaga agacggaggc gctgctcgca cagatggaac cggatgccca
     1081 cgggctcacg ttcctgccgc tgctcgccgg ggagcgtgga ccgggatggg ctgacagggc
     1141 caacgggaca gtggccgggc tctccatgag cagcgagcca gtcgagatcc tgcgcgcggc
     1201 gatggaggcc gtggccttca ggttcgccat catcgcggag atcatcgaaa ccgcctctcc
     1261 aggtgagaag gaagtcgtcg ccagcggcgg tggcctcctc aactcgccca cctggaccag
     1321 gatcattgcc gacaccctgg gcaggcccgt cacgctctcc ggcgtcgatg aagcatccag
     1381 ccggggcgcg gccctgatcg cccgcgaagc cctcggagga ccggagatcg aggaggcgcc
     1441 gtcgccgctc ggggagacct tcgagcctga ccccggacgg cacgaggtct acaggaacgc
     1501 gctcgaacgc cagcgccagc tctacgaagc ggtgcttgat cgggggtagg tttcaggttt
     1561 caggtttcag gtgcgggaag gggtttggtt cgcggtcata ggtagacatc cgttgcgcgg
     1621 cgcgcaacgg atgtcttacg tcgtgggcga ggaggtccgg ttcagttcct gagttgtccg
     1681 agcgtgtttc ggagttgctc ggcggcgtcg gaagctgcgg cgagatcgtc ctccccggcg
     1741 gcaacctgta gatccatgaa cgccgtgttg acgcgggttg cgtcggcacc atcgagagag
     1801 ctcatgatac ggtccctgat gtagcccagg gtgaagacgt cgcccctgac cgcgttggca
     1861 tcgccagccg ccgcgtcgag cgtgagctgg gcggcccaga ggtcgaagcg ggcgaggtcg
     1921 atctcggcct gtggcctgaa ttgcaaacgg agatccaggc tccattgggc ggcatcgatc
     1981 gccgcctggc tagtctgctt tgtgtcgcgg gcttcgacag cttcggtgag atcagcgagt
     2041 gcggcgctca tgcgaggctc gatcttcttt ggaacctcgc cctccctgta gtccttccat
     2101 gcggcgttca tctcctcgac cgtggtggta gccccttccc aatcttccga ctccgccgca
     2161 tcgaagactt cgagcgcgcc gctggagaga gtatccagct cggccggtgt tggacccgag
     2221 agggcatcag tcggaattgc gagggcgagg gcctcgacgt cgcctcctcc ggcggtgtag
     2281 aactcgccgt aaccgggggc gaaggtcttg ccctccttgg tgttatccat gtggagctcc
     2341 tcgacgacca gtcctccgtc cacgggaccc attggacctt cgagcgtctt gcccacggac
     2401 ttgacggtta cttcctcgaa aacgaagccg gggatgttct cgggcctgta aacgtcaccc
     2461 atttttggct ctgaaggcat gatcatcgcc gcagggccgt ccttgcccgc gatccaggtt
     2521 ccctcggtgt tggcgatggc cccgtcctcg aagttgaaca cgtcctcgcc aaaataccag
     2581 actgacccgt cgtctgcctg ggcgtagagg tcgtacgcga tctcgtggat gcgcccgtcg
     2641 agataggccg tgtactgcga aacgagcgtc tcgacgcgct ggccctgcca ttcgatgatc
     2701 ctggtctctg gcaacagcgt gacctcggtc cggaacggct tgtcatcgac ccggccaacc
     2761 ataaggaccg actcctgctt cgagaccgga aatagcgggt tggtgatctc ggtagggttc
     2821 gagaagactg gcatgctgat atctacccgc gctccttcgg gcgccgtcgc aaggtcctct
     2881 agcatgacag gctggggaat ggtctttgat ccgccctccc cttctgccgc cttggagccg
     2941 actgcggcat cgcgggaact ctgatccttc gtggccaccg tgtcggctcc acacgcgcct
     3001 atgactgaca ggccggccaa tacaactatt atcgcaacga cgcgcgcaaa tggtccctta
     3061 tgcttgccac ccatgactcc tcctgtacag ccgcgtccgc ccatgccatt gtccacgggc
     3121 ggcacgtcag acaataattc tctccaggat caatcctcgt tcggaccttc ggagtcgttt
     3181 ggcccatcgt cgcctgctga ccggccaacg acgtcgagcg tccgatcgag atgcacctcg
     3241 acctggctgc cgtccgaccg ctcgacctcg acctcgaagg cgccatctcc gtccgaccgt
     3301 tcgacttcgg taacggttcc gccacccacc gcccggagtg cggcgctctt ggccttatcg
     3361 gcgtcgggcc cggtgacctt ctccccctgt ccgacggcgt aggcggctcc gcccgcgccg
     3421 agagccagca cgaccgcaac ggcgctcgca gcgagctttt tcttgtccat attctcctcc
     3481 actcgtcggg ggctcatcta gatggagtat tccgcc
//
LOCUS       DATAIR010000812         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_368025, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000812 DATAIR010000000
VERSION     DATAIR010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368025"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VHM69_19290"
     CDS             complement(<1..514)
                     /locus_tag="VHM69_19290"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742593.1"
                     /translation="MPRITLIGAGSAVFTRNLCSDILLAPVLQDSTISLMDIDEGRLR
                     TSRDLVQSIIDRRGIDARVEATTDRREAIRDADYVVTTFQQGGLDAYALDIEIPQRYG
                     VEQCVGDTLGPGGVFRALRTIPVLIDLCDEMDELAPDAMLLNYVNPMAANCWAVDVAT
                     GRPHVGLCHSV"
     gene            complement(595..1401)
                     /locus_tag="VHM69_19295"
     CDS             complement(595..1401)
                     /locus_tag="VHM69_19295"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX2742594.1"
                     /translation="METTKHISQEQVGSFHEDGYLVVEGLFAPEEVRALADNFMEMHS
                     GGPIPGCFEPASPEEADGDILKLYPRMMHPHRVNELAMRYLLDDRLEDVLTGLFGEEP
                     LAAQSMLYFKPAGAKGQALHQDNFFLKVEPGTCIAAWVALDAADPENGGLEVVPGTHD
                     MELFCPEEADSEVSFTRDYVPVPEGLETVPVVMRPGDVLFFNGQLVHGSQPNTSEDRF
                     RRSFICHYIGRSSERISHWYRPILTMEGEEVRIEESADGGPCGTEDVGVH"
     gene            1479..2360
                     /locus_tag="VHM69_19300"
     CDS             1479..2360
                     /locus_tag="VHM69_19300"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2742595.1"
                     /translation="MAEKLYYPAMSLPEVPSPPPGVLVTGHFREEFGYRVRRPSGSGN
                     WLLTYTLDGKGLYLGSGPDLLVEPGDVVLLGPGFPHDYSVPEGGFWEFLWAHFQPRLE
                     WFEWWYPLVAGEGLSLARLESPRARERARRSFFRLHADALAGRELSRELALNGLEEVL
                     LLAVREGAGGRPVDPRVRRVLDLISSDLAATHDVPGLAKEVALSPSRLSHLFKQETGD
                     SVMGTVIQLRLSQAARLLEHTVDDIGSIARDVGFDSPYYFSRRFRRHFGMSPRQYRAK
                     SGDTRAGSPDPLRTVSE"
BASE COUNT          429 a          835 c          821 g          496 t
ORIGIN      
        1 ggacgctgtg acagagcccc acgtgcgggc gtccagtcgc cacgtccacg gcccagcagt
       61 tcgccgccat cgggttgacg tagttcaaca gcatggcgtc cggggcgagc tcgtccatct
      121 cgtcgcacag gtcgatcagc acaggaatcg ttctcaacgc gcggaagacg cctcctggac
      181 caagcgtatc gcccacgcat tgctcgactc cgtagcgctg ggggatctcg atgtcgaggg
      241 cgtaggcgtc gaggccgccc tgctggaacg tggtcaccac gtagtcagca tcccgaatgg
      301 cctcccgcct gtcggtggtt gcttcgaccc tggcgtcgat cccgcgcctg tctattatgc
      361 tctggacgag gtcgcgggag gtgcgcagcc tgccttcatc tatatccatc aatgagatcg
      421 tgctgtcttg cagcacaggg gcgagcagga tgtccgagca caggttgcgc gtgaagacgg
      481 cgctccccgc gccgatgagg gtgatcctgg gcactagcgc gtccctttcg ttcggggtcc
      541 tatcagaccg acggtacatg ccctgcgggc caggatcaag ccagtctcta gctcctaatg
      601 gacgcctacg tcctccgtac cgcagggacc gccatcggcg ctctcctcga tcctgacttc
      661 ctcaccctcc atcgtaagga tcggccggta ccagtgggag atgcgctcgg aggaacggcc
      721 tatgtagtgg cagatgaagg agcgccggaa acggtcctcg ctggtgttgg gctgcgagcc
      781 gtgtacgagc tggccattga aaaagagcac atccccaggc cgcatgacga cgggcacggt
      841 ctcgagtccc tcgggcacgg gtacgtagtc gcgggtgaag gagacttcgg aatccgcctc
      901 ctcgggacag aatagctcca tgtcgtgtgt accggggacg acctcgagcc cgccgttctc
      961 cggatccgcg gcatcgagcg cgacccacgc ggcgatacag gtgcccggct cgaccttgag
     1021 gaagaagtta tcctggtgga gcgcctgacc cttcgcccct gcgggtttga agtacagcat
     1081 gctctgcgcg gcgagcggtt cctcgccgaa gaggccggtg agcacgtcct ccagccggtc
     1141 gtcgaggagg taccgcatgg ccaactcgtt gaccctgtgc ggatgcatca tgcgcggata
     1201 gagcttgagt atgtcgccgt ccgcctcctc cgggctcgcc ggctcgaagc agccagggat
     1261 gggccctccc gagtgcatct ccataaaatt gtccgccagc gcccttacct cctcgggcgc
     1321 gaagagtccc tccactacca ggtagccgtc ctcgtggaag ctaccgacct gctcctgtga
     1381 aatatgcttc gtggtttcca agatcctaat cctccaaatg ttcctaccca ctccacacga
     1441 gatgctagcc caggggcccg atccacggaa tagtctcgat ggctgaaaag ttgtactatc
     1501 ctgctatgag cctaccagag gtcccgtcgc cgccgccggg cgtactcgtc acgggacact
     1561 tccgcgagga gttcggctac agggttcgca ggcccagtgg ttcggggaac tggttgctca
     1621 cgtacacgct ggatggaaag gggctgtacc tggggtccgg tcccgacctc ctcgtcgagc
     1681 ccggcgatgt cgtgctgctc ggcccaggct tcccgcacga ctactccgtg cctgagggtg
     1741 gcttctggga gttcctgtgg gcccatttcc aaccccggct ggaatggttc gagtggtggt
     1801 atccacttgt cgcgggagaa ggcctgagtc tggcgcgcct ggagagcccg cgtgcgcggg
     1861 agcgagcgcg gcggtccttc ttcaggctgc atgcggacgc cctcgccggc cgtgagttgt
     1921 cgcgtgagct ggcgctcaat ggtctggagg aggtgctact gctcgctgtg agggaggggg
     1981 ccggggggcg gcccgtggac ccgagggtga ggagggtcct ggatctcatc tcctccgatc
     2041 tggcggctac ccacgacgtg ccagggctcg cgaaggaggt cgcgctctca ccttcgcggc
     2101 tctctcacct cttcaagcag gagaccggcg actccgtgat ggggaccgtg atccagctgc
     2161 ggctaagcca ggccgccagg ctcctcgagc ataccgtcga cgacatcggc tcgatcgcca
     2221 gagatgtagg attcgactca ccctactact tcagccggcg attcaggcgg cacttcggca
     2281 tgagcccgcg ccagtacagg gcgaagtcag gggacacacg cgcaggctcc cccgaccctt
     2341 tacggacagt ttcagaatga tggattctcg ggtgttcgtg gggccggcgc tctcatgttc
     2401 cggccctgta gctatatagc ctgcttgccc tactgccccg gtgccctgaa aggcgtacct
     2461 ccagctctgc gcattatctc catgccctcc tcggcactga acagcggggt ggtcttcgtc
     2521 gccatcaggt gcccgcgtct ggccgcagcc aacaccgcag cgccggcagt agcgtcgtcc
     2581 g
//
LOCUS       DATAIR010000813         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_275076, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000813 DATAIR010000000
VERSION     DATAIR010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275076"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..990
                     /locus_tag="VHM69_19305"
     CDS             145..990
                     /locus_tag="VHM69_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2742596.1"
                     /translation="MRSRRARGRIGDLLTYLVFAGALVFFGGSLLWVLSLSIRTQQEV
                     YITSLRLIPEAPTLENYYEVLYSAQFSVFLINSLKLSVAGSVGAMVVAAPAAYAFSRL
                     DFRGNGALLIGVLALQMISPLVVIIPLYRYFARLNLLDSHFSTSMVYIAILVPIATWM
                     LKGFFDGIPPALDEAAMVDGCTRFGAFLRVVLPLILPGLTSVFVLTAILAWGEFIVPY
                     ILLSKPSLLPISIGILNFQGNYAQTSTQVVAAGGVLAMLPAIATFVILQRFIVRALTS
                     GAIKG"
     gene            1060..>1750
                     /locus_tag="VHM69_19310"
     CDS             1060..>1750
                     /locus_tag="VHM69_19310"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742597.1"
                     /translation="MIVHMIGNAHIDPVWLWTWQAGVDEALATFHSAADRCDEYPEFV
                     FTRGESWLFEQVERIDPGLFERVRRQVESSQWHITGGQYLQPDVNLSTVMGLHRQIIH
                     GQRYFKDRFGVSPKVGYNVDSFGHPATMPDVLDSHGYVGYVFHRPGAHQVELPAQTFR
                     WRGAGGGEVIGFRISPAYVTFSDDLYGQIMLSIEAADPELGHTMCFYGVGNHGGGPTK
                     ANIEYILENVHS"
BASE COUNT          299 a          558 c          526 g          367 t
ORIGIN      
        1 ggaggtgctc gccctccaca cgtacaacgt catcttcagg gagtacagcc tggctggcgg
       61 ggccgtgcta gccgtgatca tgctgctcat cagcctgacc ctcaccatcg cttacaggag
      121 attgctgcgt tctgaggggg cggtatgagg agtcgcaggg ccaggggcag gataggcgac
      181 ctcctgacct acctggtgtt cgccggcgcc ctggtgttct tcggcgggtc gctactctgg
      241 gtgctctcgc tctcgatcag gacccagcag gaggtctaca ttacgtcgct gcgcctgatc
      301 cccgaggccc cgacgctcga gaactactac gaggtactgt actcggcgca gttctcggtg
      361 ttcctgatca actctctcaa actctcggtg gcggggagcg tcggcgcgat ggtggtcgcg
      421 gcacctgctg cctacgcatt ctcccgcctg gatttccgtg gcaacggcgc gctgctgatc
      481 ggcgtgctcg ccctgcagat gatttcgccg ctggtcgtca tcattcctct gtacaggtac
      541 ttcgcccgcc tgaacctcct ggactcgcac ttcagcacgt cgatggtcta catagccatc
      601 ctggtgccga tcgcaacctg gatgctgaag ggtttcttcg acggcatccc tcccgctctc
      661 gacgaggcgg cgatggtgga cggctgcacg cgcttcggcg cgttcctgcg tgtcgttctg
      721 ccgctcatcc tgccaggcct tacttcagtg ttcgtgctca cggcgatcct cgcctgggga
      781 gagttcatcg tgccttacat actcctcagt aaaccctcgc ttctcccgat ctccatcggc
      841 atcctcaatt tccagggcaa ctacgcccag acctcgaccc aggtggtcgc agcgggaggg
      901 gtgctggcca tgctgcctgc catcgccacc ttcgtcatcc tgcagcggtt catagtccgc
      961 gccctgacgt ccggagccat caaaggctag gtcggggagc gagccgtctc tgtccggcgg
     1021 ttaggaatag caagctcatg caaaccgggg ggattggtca tgatagttca catgatagga
     1081 aacgcccaca tcgaccctgt gtggttgtgg acctggcagg ccggggtgga cgaggcgctg
     1141 gccacgttcc attccgccgc ggaccgctgc gacgagtacc ccgagttcgt attcacccgc
     1201 ggggagtcct ggctcttcga gcaggtggag cgaatagatc ctggactctt cgagcgggtg
     1261 cggcggcagg tcgagagcag ccagtggcat atcacgggag gacagtacct ccaacccgac
     1321 gtcaacctct ccacggtaat gggcctccac cgtcagatca tccacggcca gcgctatttc
     1381 aaggaccgct tcggagttag cccgaaagta ggctacaacg tggactcctt cggacaccct
     1441 gcgaccatgc cggatgttct ggacagccac ggttacgtcg gatacgtctt ccatcgtccc
     1501 ggagcccacc aggtggagtt gccagcacag acattccgct ggcgtggcgc cggcgggggg
     1561 gaggtgatcg ggtttcggat ctcccccgct tacgtcacgt tctctgacga cctgtacggt
     1621 cagatcatgc tctccatcga ggcggctgac cccgagctcg ggcacacgat gtgcttctac
     1681 ggggttggca atcacggcgg cggaccgacg aaggcaaaca tcgagtacat acttgaaaac
     1741 gtccactcat
//
LOCUS       DATAIR010000814         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_172039, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000814 DATAIR010000000
VERSION     DATAIR010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172039"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VHM69_19315"
     CDS             complement(<1..1073)
                     /locus_tag="VHM69_19315"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX2742598.1"
                     /translation="MPESPARRAGRIARVGARYGFGFVFRSRLLPRRRREEPGAVGTR
                     LRYSLEELGPTFAELGRFLSFRRDLIPHDVAAELGRARVVTTPTTFAEVRALVERELG
                     NTLERLFIEFEEIPVRVGPFTQSHRVLLPGERPALMVFNRPGVRRDLLAMRPVADLTR
                     RRLGDRLPLDPSATVAEFTAHVTHRRDMFFAAQISRRLRELEDFLLVPDVYRDYSTGR
                     CVTFEAPPDIASPEDSQYEEVSESLVRLVLTEGVFLADLSPGRFVIDGSTGEVWLSDP
                     TEAFSMDPERLRGAAEVLAAVRRGDVDGMVRTLPLAGSFVPRDGSDLRRELRETMGSL
                     GGPLWREHSLEEVRDRGLEAIRRG"
     gene            1292..1672
                     /locus_tag="VHM69_19320"
     CDS             1292..1672
                     /locus_tag="VHM69_19320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2742599.1"
                     /translation="MRYVHTCYRVLDLDRSIDFYTNKLGMELARKVPIGDDATNAFFA
                     VPGDPEPRLELTLNHDQDEPYELGTGYSHVAFAVEDLDAFVDRLEEAGGVDFESKPHA
                     MGSGTRIFFVKDPDGYRIEFIERP"
BASE COUNT          342 a          593 c          555 g          283 t
ORIGIN      
        1 cctctacgga tggcctcgag gcccctgtcc ctgacctcct cgagggagtg ttcgcgccac
       61 agagggccgc ccagcgagcc catggtctcg cgcaactcgc gcctgaggtc cgagccgtct
      121 cggggtacga aactccctgc cagcggcagc gtgcgaacca tcccgtccac gtccccccga
      181 cgcaccgcgg ccagaacctc cgctgcgccc cgcagcctct ccgggtccat gctgaacgcc
      241 tccgtcgggt cggagagcca gacctctcct gtgctgccgt cgatgacgaa gcgcccgggc
      301 gacaggtccg cgaggaagac tccctcggtc agcacgagcc gcaccagaga ttcggagacc
      361 tcctcgtatt gactgtcttc cggggacgca atatccgggg gcgcctcgaa cgtgacgcag
      421 cggcccgtcg agtaatccct gtagacgtca gggacgagca ggaaatcttc cagctctctc
      481 aggcggcggg agatctgggc ggcgaagaac atgtcgcggc ggtgggtgac gtgcgcggtg
      541 aactccgcaa cggtcgcgga ggggtcgagg ggcagcctgt cgccgagcct ccggcgcgtc
      601 aggtcagcca cggggcgcat cgccaggagg tccctgcgca cgcctgggcg gttgaacacc
      661 atgagcgcgg ggcgctctcc cggaagcagg acccggtgcg actgtgtgaa cggaccgact
      721 ctgaccggta tctcctcgaa ctcgatgaac agtcgctcca gggtattacc aagctcccgc
      781 tcgacgaggg cacgtacctc ggcgaaggtg gtaggcgtcg ttacgacccg cgcgcgcccc
      841 aactcggcgg ccacatcgtg tgggatcagg tctcgacgga aggagaggaa gcgccccaac
      901 tccgcgaacg tcgggcccaa ctcttcgagg gagtagcgta gcctcgtgcc gaccgccccc
      961 ggctcttccc tgcggcgacg cggcaacaga cggctcctga acacgaagcc gaagccgtag
     1021 cgcgccccga cacgtgcgat cctgcccgcc ctgcgggccg gactctcagg caaaccctgc
     1081 gtcaccttct ccatgaacac agcttatcag cacgccgcct tcggcggctt gtcagccagt
     1141 cagcacgctt cggcccaagg cctccgcttg tcagctattc agcaacggag gaggtattga
     1201 gactgccgtt cgacgaaatg ctgaggctga aagcgaaggc gccagcctca gcgtgctgat
     1261 aagctgaagt gctggcgacg aaggagcgaa gatgcggtac gtacatactt gttacagggt
     1321 cctggatctc gacaggagca tagatttcta cacgaacaag ctgggcatgg agctcgcccg
     1381 aaaggtccct atcggtgatg acgcgacgaa cgccttcttc gccgtgcctg gcgaccccga
     1441 gccacgactg gaactcaccc tcaaccacga ccaggacgaa ccctacgagc taggtaccgg
     1501 gtatagccac gtcgccttcg ccgtcgagga tctcgacgct tttgtcgaca ggctagaaga
     1561 agccggcggc gtggacttcg agagcaagcc gcacgccatg ggcagtggga ccaggatctt
     1621 cttcgtcaaa gaccccgacg gctaccgtat agagttcatc gagcgcccgt aacgaactca
     1681 tcgggctcgt gagagcccgc taaagtatgt ccatctccgc gccgacaaag aggtcgtgcg
     1741 cgcgatacgg ccgaaacttg cccgcgtttg tga
//
LOCUS       DATAIR010000815         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_80215, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000815 DATAIR010000000
VERSION     DATAIR010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80215"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VHM69_19325"
     CDS             <1..731
                     /locus_tag="VHM69_19325"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2742600.1"
                     /translation="LSNVDVRDVVKPSALADALGTVLAGVAGFLSDLFLILMIMLFLL
                     GEGPAMMERLRTSTSSENQQVERLTTVGRSVVRQFGLRAIVNLVTGAGVTVMLFALGV
                     DFPLLWGILTFFLSFVPYIGLVLAVTPAVVLALAEFGVSRAVLVIIGVIVINVLAENV
                     LSPLMMGRGLNISPTVVFLSFIVWAWLLGGPGAFLALPITLFVAVMFDTFPETRWLAS
                     LIGVADAGDGAPPDASTREAEHAS"
     gene            786..2504
                     /locus_tag="VHM69_19330"
     CDS             786..2504
                     /locus_tag="VHM69_19330"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742601.1"
                     /translation="MQSSDDVKKIRIRAMEELGAIPRVLKELQELVPLLSAAYSTDGR
                     TFGYEAPLSMSIPVGSYVSLTAQGGGEYLGQVVTKEVSVRKGAEVGLELDPSLSALLP
                     EGISFSSSHTYPLQRLYAHGSGVILGKLEQGRYVPTSSEDNFQSADLAPAGESLVAAY
                     LAGGVRAQLDVGRALYTEGRVRVPLKAEGFDRHTFLCGQSGSGKTFALGVILERLLLE
                     TELKIVILDPNSDFVRLDRVRPRDDVNRTSSSALSSEGYDALLVRYGRVAPRMRLLRP
                     APYAEDPSKLLRIRFSDLERHEQGLVLGMDPLRDREEFNAYWRAIQDLNRDRYSLSEV
                     KEVVVRRFSEDARRLGLRIENLGIADWSLWCESDKPSLVDMLEGDWRCLIVDSGTLAS
                     PAEQSIVAMAVLGHLWRYRNERQPVLIVADEAHNICPQEPADNLEAAATSHAIKIAGE
                     GRKFGLYLLVASQRPSKIHANVLSQCDNLALMRMNSVSDLAHVSQIFSQAPATFLEQS
                     SHFAQGECLLAGKMVRNPTFVAFEGRFSEEGGTDVPSSWASVDGNQVLQSPDGGGSLA
                     SRGGQQ"
     gene            2635..3573
                     /locus_tag="VHM69_19335"
     CDS             2635..3573
                     /locus_tag="VHM69_19335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2742602.1"
                     /translation="MLQGIGVIELGERSVRNFFEHRMTTYAAALAYRGLFGLFPFVLI
                     LVVLTSALGLSAFIERSFEQQSKQNVPQQLEPVVEQGREQIQPLERMVEQAEKQARGE
                     LLIFGVGVALWSISALAHTLTDAFNVAYETSETRHGWKWFLLSWAFGPVLALVVLVAI
                     GLMLVGPRLIERITELVGLDEVFVLLWGWLRFPVALSLLGVVLSLVYRFGPDAGVRFR
                     SVRLGAALAVLLWAISSVGFSFYLANFADYGVTYGSLGAAVGLLFYLYLSASVVLLGA
                     EVNATVYHAASDSTKVVEIHGDPDNNAADTRRMKDV"
BASE COUNT          684 a         1167 c         1210 g          771 t
ORIGIN      
        1 ggctctcgaa cgtcgacgtt cgagacgtgg tcaagccaag cgccctcgcc gatgcccttg
       61 gcaccgtact cgccggcgtc gcggggttcc tgagcgacct gtttttgatc ctcatgatca
      121 tgctcttcct gctcggcgag gggccggcta tgatggagcg tctgcgaacg agcacgagta
      181 gcgagaacca gcaggtcgag cgcctgacca ccgtcggtcg cagcgtcgtg cggcagttcg
      241 ggctgcgcgc catcgtcaac ctcgtcacgg gcgccggcgt aaccgtcatg ctcttcgcgc
      301 ttggggtgga cttcccactt ttgtggggga tcctgacctt cttcctgagc ttcgtgccgt
      361 acatcggttt ggtgcttgcg gtgacgcccg ctgtggtact ggcactggcc gagttcgggg
      421 tgagccgggc tgtgctggta atcatcgggg taatagtgat caacgtgctc gccgagaacg
      481 tgctctcacc cctgatgatg ggacgagggc tcaacatctc gccgactgtc gtcttcctgt
      541 cctttatcgt ctgggcgtgg ctgctagggg gccctggtgc gttcctggcg ctgcccatca
      601 cgctcttcgt cgccgtaatg ttcgacacct tccccgagac gcgctggctt gcgagcctca
      661 tcggcgtggc tgacgccggg gatggtgctc caccggacgc ttctacccgg gaggccgaac
      721 atgcgtcctg agaagaaagt ccggatcgat gcatcaacag tagggtgtct gagagggtga
      781 gcggcatgca atctagtgac gatgtgaaga agatccggat cagagccatg gaagaactcg
      841 gcgcgatacc gagggtgttg aaggagctac aggagcttgt tccactactc tctgccgcct
      901 actcgaccga cgggcggacg ttcggctacg aagctccact ctcgatgtcc atccctgtcg
      961 gttcctatgt gtccctgacc gcccagggcg gcggtgagta cctcggccag gtcgtcacca
     1021 aggaggtgtc tgtccggaaa ggggcagagg tcggcctgga actggacccg agtctcagcg
     1081 ctctcttgcc agagggaata agcttctcct cgagccacac ctaccctcta caacggctct
     1141 acgcccacgg ctccggcgtc atcctgggca agctggagca gggtagatac gtaccgacga
     1201 gcagcgaaga caactttcag agcgccgacc tcgcgcccgc gggcgagagc ctggtagcag
     1261 cgtacctcgc tggcggagtg cgggcgcagc tcgacgtcgg tcgggcgctg tacacggaag
     1321 gtcgggtgcg agtaccactg aaggcggagg gtttcgacag acacaccttc ctgtgcggcc
     1381 agtcgggatc gggcaagacg ttcgcgctgg gtgtgatcct cgaacgcctg ttgctcgaga
     1441 cggagctcaa gatcgtcatc ctcgatccca actcggactt cgtccgcctg gatcgggtgc
     1501 gtccccgcga cgacgtgaac cgaaccagct cgagcgcgct ctcctccgaa ggttacgatg
     1561 cgctactggt acgctacggg cgggtcgcgc caagaatgag gctcctgcgt cccgccccct
     1621 acgccgagga cccctcgaag ctcctgcgca tccggttcag cgacctcgag aggcacgagc
     1681 agggtctcgt gcttgggatg gacccgctgc gtgacaggga agagttcaac gcctactggc
     1741 gcgcaatcca ggatttgaat agagacaggt actcgctctc cgaggtaaaa gaggttgtgg
     1801 ttcgccggtt ctcagaggat gcgcgcaggc tcgggctcag gatcgagaac cttggcatcg
     1861 cggactggag cctgtggtgc gagtcagaca agccctccct cgtcgacatg cttgaggggg
     1921 actggaggtg cctgatcgtg gactcgggca ccctcgcctc gcctgcggag caatccatcg
     1981 tcgctatggc cgtgctaggc cacctgtggc ggtacagaaa cgagcgccaa cccgtcctga
     2041 tagtcgcaga cgaagcgcac aacatctgcc cacaagaacc ggcggacaat ctcgaggcgg
     2101 cggcgacaag ccacgcgatc aagatcgccg gtgagggccg caagtttggc ctctacctgc
     2161 tcgttgcctc ccagagaccg agcaagatcc acgccaacgt actatcacag tgcgacaacc
     2221 tcgcgctgat gaggatgaac tcggtctccg atctcgcgca cgtctcccag atcttctcgc
     2281 aagcaccagc cacgttcctg gagcagtctt cgcacttcgc ccagggtgag tgcctgctcg
     2341 cagggaagat ggtgcggaac ccgacgttcg tggctttcga ggggcgcttc tcggaagagg
     2401 gaggaaccga cgtgccttcc tcatgggcgt ccgtcgacgg gaaccaggtg ttacaaagtc
     2461 cggacggcgg cggatccctc gcatcacgag gtggtcaaca atgaccgggg cgccggcgtg
     2521 gagcgaggaa gctacattgc ttgcacgctt cgtctccgac gtggaggttc ggggccgaag
     2581 ccaccctggc cacggtgttc cttcacctca aggtaggatg gtggacacgt tctgatgctg
     2641 caagggatcg gggttataga actcggcgag cgctcggttc ggaatttctt cgagcaccgc
     2701 atgacgacgt acgccgccgc gctggcgtac cgtgggctct tcggcctctt ccccttcgtg
     2761 ctgatcctgg tcgttctaac gagcgcgttg ggcttgtcag ccttcatcga aaggtccttc
     2821 gagcagcaat cgaagcagaa cgtgccccag cagctagaac ccgtcgtaga gcaaggcagg
     2881 gagcagatcc aacccctcga aaggatggtc gagcaggccg agaagcaagc tagaggagag
     2941 ttgctcatct tcggggtggg cgtcgcgcta tggtccatct cagcgctcgc ccataccttg
     3001 accgatgcct tcaatgtcgc ctacgagaca tctgagaccc gtcacgggtg gaaatggttc
     3061 ctcctttcct gggcgttcgg tcccgtcctg gcgcttgtcg tgctcgttgc catagggctg
     3121 atgctggtcg gcccgcgcct gatcgagcgc ataacggagt tggttggcct ggatgaggtg
     3181 ttcgtcttgc tgtgggggtg gctgcgcttc cccgtcgccc tttctctgct gggcgtggtg
     3241 ctctcactcg tctaccgctt cgggccagac gccggagtgc gcttccgctc ggtgcgcctt
     3301 ggtgccgccc tcgccgtgtt gctctgggcg atatcctcgg tcggtttctc gttctacctt
     3361 gcgaacttcg cagactacgg cgtcacgtac gggagcctgg gagcggccgt gggtctgctc
     3421 ttctacctgt atctctcagc ctcggtcgtg ctgcttggcg ctgaggtgaa cgcgacggtc
     3481 taccacgcgg catcggacag cacgaaggtc gtggagattc acggagatcc ggacaacaat
     3541 gcagccgaca cccggaggat gaaagatgtc tgaagcaaca gcccggtcga gccccgactc
     3601 ttctgcggat gcgctggcat tgttccgcgc ccggcggttc atcacggcta cgataacatc
     3661 gatggatagg ttggacgctg acctggctga gaccttaaat ccgtgtgaca tagcaactgt
     3721 gcaccgagag gagagaattt gaacctgcaa atcgtaccgc tcgccatcac gatgatggca
     3781 ggaccacaga tcatgtcggc catcatcttc atcactgccc tcaaacccct ta
//
LOCUS       DATAIR010000816         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_172381, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000816 DATAIR010000000
VERSION     DATAIR010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172381"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VHM69_19340"
     CDS             complement(<1..424)
                     /locus_tag="VHM69_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742603.1"
                     /translation="MRVGPFEREHLPLLLNLVNLHLAAVVPGLAISEAFLAGHLHRNL
                     DEYITDPWVEERATLCAVEGHRMLAAAHLLRYGDDLQVGEYLRDVGEVAWFVFLPDRD
                     DAAGEVLSRVRGRLAAWRVTRRQAYGAGLPKMPLLGIPE"
     gene            complement(558..>1658)
                     /locus_tag="VHM69_19345"
     CDS             complement(558..>1658)
                     /locus_tag="VHM69_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2742604.1"
                     /translation="ADFEEYARIYRDAWGDPAIRWLLSTVPSAMIFDDHEVGDDWNIS
                     ASWVEEMRCQPWWNDQIVGGYASYWIYQHLGNLPPEELEKDDLYAKVKDAGDAWPILQ
                     DFAYHTHREPDGARWSYHRDFGATRLLMMDSRGGRVLEEEHRSMVDAKEWAWIEDKAT
                     GGFDHLLLGTSIPVLLGPGMHHLQSWTERLCYGVWGKRMASWAEGLRRSQDLDHWPSF
                     HDSFAALVERIRAVAGGEEGTPPSTVMILSGDVHHGYLAEATFDGGAESRVYQAVASP
                     FRNALPGKKSRLQANAWKKPAVLAGRLLARFSGVEEEPLTWRLTHEAAWFDNQVATLE
                     LRGRQATITFEKAVLGDSQEPGLEKIYSRRLA"
BASE COUNT          289 a          514 c          562 g          293 t
ORIGIN      
        1 cttcgggtat gcccaacaac ggcatcttcg gcagcccggc cccgtaagcc tgccggcgtg
       61 tgacacgcca ggccgcgagc ctcccgcgga cgcgagacaa tacctcgccc gccgcgtcgt
      121 cgcggtccgg tagaaaaacg aaccaggcga cctcgccgac atcccgaagg tattctccga
      181 cctggaggtc gtccccgtaa cgcaagagat gagcggcggc gagcatacga tgtccctcca
      241 cggcacacag cgtcgccctc tcttccaccc agggatcggt gatgtactcg tcgagattgc
      301 ggtgcagatg accggccagg aaggcttccg agatcgccag gcccggcacg acagcagcga
      361 ggtgcagatt gacgaggttt aggagaagcg gcagatgttc tcgttcgaag ggtccgacac
      421 gcatgatgta tctcctctcc aaagacaacg gacagcgggg tcggggccgt cccggaggag
      481 aacgcgctcg tcgtatgggg ccgggtcaga tcaaggccgt agcccaacat cagtcgagtc
      541 ttggcgcctt cgggtcatca tgccagccgg cgggagtaga tcttctccag tcccggttct
      601 tgcgagtcgc cgaggacggc cttctcgaag gttatggtgg cctggcggcc tcgtagctcc
      661 agcgtcgcta cctggttgtc gaaccaggcg gcttcgtggg tgaggcgcca ggtgagcggc
      721 tcctcttcga caccggagaa gcgcgcgagc aggcgtccgg cgaggacggc gggcttcttc
      781 caggcgttgg cctggaggcg ggacttcttg ccggggaggg cgttgcgaaa gggtgaggcc
      841 accgcctggt aaacgcggct ctcggcgccg ccgtcgaaag tggcttcggc caggtatccg
      901 tggtggacgt cgccggagag gatcatgacg gttgacggtg gcgtgccttc ctctccgccc
      961 gcgacggctc gtatgcgctc gacaagcgcg gcgaaggagt cgtggaagga gggccagtgg
     1021 tcgaggtcct gcgagcggcg aagtccttcg gcccacgagg ccatacgctt gccccatact
     1081 ccgtagcaca gcctctcggt ccagctctgg aggtgatgca ttccggggcc gagcaacacc
     1141 gggatagagg tgcctaacag caggtgatcg aagccgccgg tcgccttgtc ttcgatccag
     1201 gcccactcct tcgcatccac catcgagcgg tgttcctctt cgaggacgcg cccgccgcgg
     1261 gagtccatca tcaagaggcg cgtggcgccg aagtcgcggt ggtagctcca gcgggcgccg
     1321 tcgggctcgc ggtgggtatg ataggcgaag tcctggagga taggccaggc gtcgccggcg
     1381 tccttcactt tcgcgtatag gtcgtccttt tcgagttcct cgggtggaag atttccgagg
     1441 tgctgataga tccagtaaga ggcgtagccg ccgacaatct ggtcgttcca ccagggctga
     1501 cagcgcatct cctcgaccca cgaggcggag atgttccagt cgtcgccgac ctcgtggtcg
     1561 tcgaagatca tggccgacgg gacagtggag agcaaccacc ggatcgcggg gtctccccaa
     1621 gcgtcgcggt agatcctggc gtactcctcg aagtccgc
//
LOCUS       DATAIR010000817        11270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_276012, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000817 DATAIR010000000
VERSION     DATAIR010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276012"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1785)
                     /locus_tag="VHM69_19350"
     CDS             complement(151..1785)
                     /locus_tag="VHM69_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2742605.1"
                     /translation="MPELVLGPLLRYAGSTDATVWVETDAACEVEVLGCSSRTFRVKD
                     HHYALVHVTGLSPGEASEYEVRLDGEKVWPEPETLFPPSVIRPMENGETIKLVFGSCR
                     ISAPHEPPFTLSHEEDARGLGVDTLYATAMRLQEELPEELPHALVMLGDQIYAHKPPF
                     DTLEFIKERRDTERPPGEVVAKFEEYARIYHDAWRDPAIRWLLSTVPSAMIFDDHEVG
                     DDWNISAAWVEEMRQQQWWNDQIVGGYASYWIYQHLGNLSPEDLDGDDLYRKVREVDD
                     AWHVLRDFAYRTHRGADGSRWSYHRDLGNTRLLMMDSRGGRVLEEGRRSMVDADEWAW
                     IEDRATGSFDHLLLGTSLPLLLGPGMHHLQSWDERLCSGAWGERAARWAEGLRRSQDL
                     DHWASFHDSFVAMVDLLGRVARGESPPSTVLVLSGDVHHGYLAEATFTDGARSRFYQA
                     VSSPLRNALPNKKSFLPSYAWSKPVIFATRFLARLAGVRAEPLTWRMTHDAAFFENQI
                     STLELEDRSATITFEKAVLDASKEPALEVLYERRLA"
     gene            1989..2831
                     /locus_tag="VHM69_19355"
     CDS             1989..2831
                     /locus_tag="VHM69_19355"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2742606.1"
                     /translation="MTEESRAPIPARRPGMTGTARRLLRLILVVILLVGASQGTTLAA
                     PASWDYVALGDSLATGFGAFSGYVPRYETHLETDTGMTVTRTNLGQNGWTSSQLLAAL
                     RNDAKFRRAVRGAEVVTWDIGGNDLRAARNSYKNETCGGADNQDCLRAAVTKLETNWD
                     AITAEILGLRSTNNTIVRTMDIYNPYVSTDKASDTWQNDGGLNDFQVFKSYVDEANQH
                     IAATSTDSNLPYARVYLAFNGTNGGDDPRSKGYLSFDGLHPNDTGHRIIAGELRKLGY
                     APLR"
     gene            2898..3890
                     /gene="trpS"
                     /locus_tag="VHM69_19360"
     CDS             2898..3890
                     /gene="trpS"
                     /locus_tag="VHM69_19360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2742607.1"
                     /translation="MQRKRRVFSGIQPSGNLHLGNYVGALKHWVAMQDDYENYFCIVD
                     LHAITVPQDPKVLRQKIREVAAIYLASGLDPEKCVIFRQSKVSGHAELCWLLNCVARF
                     GELSRMTQFKDKTQKGGAESASAGLFDYPVLQAADVLLYNAHLVPVGEDQRQHLELMR
                     TLARRFNHLYGETFVVPEAMIPPTGARVMSLDAPEEKMSKSSPRPGSYVAILDDPGAI
                     RKKIRRAKTDSGTEIRAVPDKPAITNLLDVYSAMSGEPVAALEAEYEGRGYGDLKKDL
                     AEVVIDGLSPVRERALELLDDPAELDRLLESGARRASDAARPTLESARARLGLD"
     gene            3936..4151
                     /locus_tag="VHM69_19365"
     CDS             3936..4151
                     /locus_tag="VHM69_19365"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2742608.1"
                     /translation="MEARYIVDENGKRVSVILPVEEYERMIEELENVEDARAADEVRA
                     AVARGDDEFIPYEQAREETARGRAANR"
     gene            4175..4399
                     /locus_tag="VHM69_19370"
     CDS             4175..4399
                     /locus_tag="VHM69_19370"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX2742609.1"
                     /translation="MSASAWRSFPSKSTTGWTGRYWRSPTIPGCTRLSGRDDWRIRVG
                     DYRIVYGIDDERRVVEILNVAHRRDVYRRG"
     gene            4402..6099
                     /locus_tag="VHM69_19375"
     CDS             4402..6099
                     /locus_tag="VHM69_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HEX2742610.1"
                     /translation="MQRLRATLDRIDRKGYGAYKDLSGSYGFGHFTLFIDKVQRDPFA
                     PPSLIRVRTKENAFERDLLDNPVRRIAFEDFLTRSVERGIRNVVRGNRGSGGSGRVEI
                     QRTSQVVLPRTSMVVEPGYVEARMAVGLPARGRSVDARAARTVLLEELPEVVRRSLVP
                     TPEGGVDGERTRLHVETVEDADHLRRLLPELGLVAFVADGAVLPRESGASDLPLREGA
                     VPFASPEEFRVEVDLPNRGIISGMGIPEGVTLVAGGGFHGKSTLLSALSWGVYDHVPG
                     DGREYVVAREDAVKVRAEDGRSVSGVDISAMIGDLPGGRTTEDFSTPNASGSTSQAAN
                     IAEAIEVGTSLLLVDEDTSATNFMIRDERMRELVRREPISPFIDLVLPLHRALRVSTV
                     VVVGGVGDYLDVADRVILLEDYAPSDATRHAREVTAKFPPRAPLSHREMYPPRERIIH
                     ASSIDLRRGKRRTARGRGLGIIELGRERVDLSYLEQLAETGQTEAVARIIGRFAAAGE
                     VREMRELVREALASVSGNGLDSLGNFEGHPGEMSLPRAQEVAAAINRVRPLLASPKM"
     gene            6163..6936
                     /locus_tag="VHM69_19380"
     CDS             6163..6936
                     /locus_tag="VHM69_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2742611.1"
                     /translation="MKETAVEDTNVSNTDTPSWFRLKAVAVHLYTASGAVLALLMLVA
                     AFQGEAVKALWLMLASLLIDSTDGLLARRFRVSEALPFFDGALLDNIVDYMTYVFAPM
                     VLLWSGGYLPDGNAGIVLAALPLLASSYQFCRVDAKTEDHYFLGFPSYFNVVAFYAIV
                     FEPGPLVLAAVLVACSFLVFIPIRYVYPTRTVAFRGLSLTLTALWLASYAAILWQMPH
                     PDPLILAFSILYLVYYFGLSLYLSGKMLEARRLEEEIAD"
     gene            6990..7469
                     /locus_tag="VHM69_19385"
     CDS             6990..7469
                     /locus_tag="VHM69_19385"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742612.1"
                     /translation="MAGRASGPPTVILRDVAEGDLPVFFEHQLDPEATRMASFPARDR
                     NAFMAHWTRILSDETVFTKTILFDGRVAGNVVSFVHSGEREVGYWVGKEFWGKGIATQ
                     ALSVFLDQIETRPLYAHVARHNIGSIRVLQKCGFRISGEEPEGLILQLEANERDEAR"
     gene            7474..7788
                     /locus_tag="VHM69_19390"
     CDS             7474..7788
                     /locus_tag="VHM69_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742613.1"
                     /translation="MTDILITERSKYVTNESHHESARRTHGSQGTRRDTGGVVHQPSN
                     PGTEPTRVLLIFSPAGMDRFFEEAAEGRMPLQAVPTDPEVKERLDSFTSRYGYEFAEF
                     PQ"
     gene            7904..8932
                     /locus_tag="VHM69_19395"
     CDS             7904..8932
                     /locus_tag="VHM69_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX2742614.1"
                     /translation="MKRLIETPGVSGREERQREIAREELDTLTDEVRTDALGSVIGTM
                     KGSDDTRVMVAAHTDEIGYLVKHIDDKGFVRLQTLGGHDPANMVSQRVIITTAEGETL
                     RGALQPARKPPHLAHGEEQKPPKADEFYVDLGMDAETMKEKVRIGDYAVMDRTLEKVG
                     DNYIGKAMDDRIGVFVMYEALRAMGDHQATIFAAATSQEEVGLRGAAASGSALQPTVV
                     VALDVTLAMDVPGAGNENTITELGKGVAIKIMDGYSISHPKLVEHLRAIAEREDIPYQ
                     MEILPAGGTDAGGVQQLHGGIPAVTLSVPCRYVHTPNEMVSATDVEAAITLLARYLEE
                     AHSADYSL"
     gene            9009..9308
                     /locus_tag="VHM69_19400"
     CDS             9009..9308
                     /locus_tag="VHM69_19400"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX2742615.1"
                     /translation="MESAGVAQLKARLSEYLARVRAGEEVLVTDRGQPVARLVPVGYL
                     PDDEAELARLKAMEREGLMRLGSGRLPAGFFEKERPADPEGLLREAVLEERERGR"
     gene            9312..9749
                     /locus_tag="VHM69_19405"
     CDS             9312..9749
                     /locus_tag="VHM69_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2742616.1"
                     /translation="MRFWDTSAIMPLISREPLSEEMRRLLEEDAGIVVWWATRVECIS
                     AISRRAREGSLGTEGEASARGLLDDLSESWTEARPTTRLRALAEQALAAHPLRAADAL
                     QMASAMIWCGSQPRGRPFVCLDERLRAAASRSGFVLLPNAHTD"
     gene            9789..10589
                     /locus_tag="VHM69_19410"
     CDS             9789..10589
                     /locus_tag="VHM69_19410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742617.1"
                     /translation="MEGDSSTTVMARAIEENGAEFLMSLGRAAGAEIRDDERLRWAIG
                     NSPIDYHNCVVHAYLTPEEADEEIEVSVERMRAHGVPGSWHVGPSMRPPDLGKRLVAR
                     GFEYGGDDIGMAVDLSMLPEEVTVPEDFLVERVRDEAGLAAWVEALGSGFGEGPVEAE
                     WVGEMYRRLGEDASLRHYLGRLGGEPVATATLFIGAAVAGIYFVCTVGRARRRGIGAA
                     VTLAPLREARDLGSSVGVLGSSEMGYPVYRRLGFEEYCRIGLYEWRPG"
     gene            10667..11188
                     /locus_tag="VHM69_19415"
     CDS             10667..11188
                     /locus_tag="VHM69_19415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2742618.1"
                     /translation="MADKNYDRPPEMTLEQGYEYYANFVTEKGRVKIRLFADEAPETV
                     NNFVFLARDGYFDGTTFHRVIENFMVQGGDPTGTGTGGPGYTISDEFHPELRHDRPGV
                     LSMANAGPNTGGSQFFITHVATPWLDDRHAVFGEVIEGMDVVNAIRERDPQRDREPGD
                     KIERVEIEEVSLS"
BASE COUNT         2238 a         3400 c         3674 g         1958 t
ORIGIN      
        1 tgaggtgagt aaaggtttca ggctacaggc atcagcaaaa agacctgatg cctgtagcct
       61 tctccgaagg agtagctcat ccttttcgag aactgccata ggggaccggc cttagacgct
      121 ggagccgggg tgcagaagcc ccggctctat ctatgccaga cgccgttcgt agagcacctc
      181 cagggcaggt tctttcgaag cgtcgaggac ggccttctcg aaggtgatcg tggcgctcct
      241 gtcttcgagc tcgagggtgg agatctggtt ttcgaagaag gcggcatcgt gggtcatgcg
      301 ccacgtgaga ggctctgcgc ggactcccgc gaggcgggcg aggaagcgag tggcgaagat
      361 tacaggtttc gaccaggcgt agctcgggag gaaggacttc ttattcggga gggcgttgcg
      421 caggggggag ctgacggcct ggtagaagcg gctcctcgcg ccgtcggtga acgtagcttc
      481 ggcgaggtag ccgtgatgta cgtcgccgga gaggaccagg acggtggaag gggggctctc
      541 tccgcgggcg acgcgtccta gcaggtcaac cattgcgacg aatgagtcgt ggaaagaggc
      601 ccagtgatcg aggtcctgcg agcgacggag tccttccgcc caacgtgcgg cgcgttcgcc
      661 ccaggcaccc gagcacaacc tctcgtccca gctctgaaga tgatgcattc cgggaccgag
      721 gaggaggggt agggaggtgc cgaggagaag gtggtcgaag ctacctgtgg ccctatcttc
      781 gatccaggcc cactcgtcgg catcgaccat ggagcggcgg ccctcctcca gtaccctgcc
      841 gccgcgggag tccatcatca ggaggcgtgt gttgccgagg tcgcggtggt agctccagcg
      901 cgagccgtcc gcgccgcggt gggtgcggta ggcgaagtcg cgcaggacgt gccaggcgtc
      961 gtccacctct ctgaccttcc tgtacaggtc gtctccatcc aggtcttcgg gggatagatt
     1021 gccgaggtgc tggtagatcc agtacgaggc gtagccgccg acgatctggt cgttccacca
     1081 ctgttgttgt cgcatctcct cgacccaggc ggcggagata ttccagtcgt cgccgacctc
     1141 gtggtcgtcg aagatcatgg ccgaaggcac ggtcgagagg agccagcgta tcgcggggtc
     1201 cctccaggcg tcgtggtaga tcctggcgta ctcctcgaat tttgcgacga cctctccggg
     1261 aggacgttcc gtgtccctgc gttcctttat gaactcgagt gtgtcgaagg gaggtttgtg
     1321 ggcgtagatc tgatccccga gcatcactag agcgtgcggc aattcttcgg gcagttcctc
     1381 ctgtagcctc atcgccgtcg catagagcgt gtccaccccg aggccacgtg cgtcctcttc
     1441 gtggctcagg gtgaatggcg gctcgtgcgg ggctgagatg cgacacgagc cgaagaccag
     1501 ctttatggtt tcgccgtttt ccattggtcg gatcacactc ggcggaaaaa gagtctcggg
     1561 ctcaggccag accttctccc cgtcgagcct gacctcatac tcgctggctt cgccgggcga
     1621 gagccccgtg acgtggacga gggcgtagtg atgatccttt acgcggaacg tgcgcgagga
     1681 gcatccaagg acctcgacct cgcaggcggc gtcggtctcc acccagactg tcgcgtctgt
     1741 ggagccagcg tagcgcagta gcggcccgag gacgagttca ggcacagcag gtcacgcttg
     1801 tgtggagaat gtcttcgcgt ttcaaggaac ctactttccg aagttgaccg aaggatacac
     1861 cagacacaaa agctcacgaa ccggggtgcc gtagagatag ctgattcgtt cagaagaatg
     1921 taccggttct ccccgatagt gtagagttct caagccacac ggcgaacgta gcccgataag
     1981 gaggaatcat gacggaggag agcagagccc cgatccccgc ccgacgacca ggcatgacag
     2041 gtacagcgcg tcgcttgctg aggctcattc tcgtcgtgat cctactcgta ggcgcgtcgc
     2101 agggaaccac cctggccgcg cccgcctcgt gggattatgt ggccctcggc gactccttgg
     2161 ccacgggctt cggcgccttc agcggctacg tgccccgcta cgagacccac ctcgagaccg
     2221 acacagggat gacggtcacg cgcaccaacc tcggccagaa cggatggacc agctcccagc
     2281 tgctcgccgc cctccgcaac gacgcgaagt tcaggcgcgc cgtccgcggg gccgaggtcg
     2341 tcacctggga catcggcgga aacgacctca gggcggcccg caactcctac aagaacgaga
     2401 cctgcggagg ggccgacaac caagactgcc tcagggcggc cgtaacgaaa ctcgaaacca
     2461 actgggacgc catcacggcg gagattctcg ggttgcgttc gacaaacaac accatcgttc
     2521 gcaccatgga catatacaat ccctacgtca gcaccgacaa agcctcagac acctggcaga
     2581 acgacggcgg gttgaacgat ttccaggtct tcaagtccta tgtcgacgag gccaaccaac
     2641 acatcgccgc cacgtctacc gacagtaact taccctacgc cagggtgtac ctggccttca
     2701 acgggacgaa cggaggcgat gatccgagat ccaagggtta tctgagcttc gacgggctcc
     2761 atccaaacga caccgggcac aggatcatag ccggcgagtt gcggaagctt ggctacgctc
     2821 ccttgagata agtccggttg ggccggcgct ttttcagtac cggcccaaat ccttccaagt
     2881 ccgttagaat gacgcgcatg caaagaaaac gacgagtatt cagcgggatt caacctagcg
     2941 gcaatttgca tctcggcaat tacgttgggg cgctcaagca ctgggtcgcg atgcaggacg
     3001 attacgagaa ctacttctgc atcgtggacc tgcacgcaat tacagtgccc caggacccga
     3061 aggtacttag gcagaagatc agggaggtag cggctatcta cctcgcctcc gggctggatc
     3121 cggagaagtg tgtgatcttc cgccagagca aggtcagcgg ccacgcggag ctgtgctggc
     3181 ttctcaactg cgtggcgcga ttcggcgagc tctcgcgcat gacgcagttc aaggacaaga
     3241 cccagaaggg tggtgccgag tcggcgagcg ccggactctt cgactacccg gtcctccagg
     3301 ctgcggacgt tctcctgtac aacgcgcacc tcgtccccgt aggcgaggac cagcggcagc
     3361 acctcgagct catgcggacg ctcgcgagga ggttcaacca cctctacgga gagaccttcg
     3421 tcgtgcccga ggccatgatc ccacccacag gagcaagggt gatgtcgctc gacgcgcccg
     3481 aggagaagat gagcaagagc tctccaagac ccggcagcta cgtagccatc ctcgacgacc
     3541 cgggcgccat aaggaagaag atccggcgcg ccaagaccga ctcggggacg gagatccgtg
     3601 cagtcccaga taaacctgcc ataacgaacc tgctcgacgt ctactcagcg atgtcgggcg
     3661 agcccgtggc cgcactcgaa gcagagtacg agggcagggg ctacggcgac ctcaagaagg
     3721 acctcgccga ggtcgttata gatggcctct cgcccgtcag ggagcgggcc ctggagttgc
     3781 tggacgaccc ggctgagctt gaccgtctac tcgagtcagg cgccaggcgg gccagcgacg
     3841 cagcgcggcc cacgctggag agcgccaggg ccaggctcgg cctcgattag cggtaagatt
     3901 tggggaccgc tccgcaagct caaggaggcg aaccgttgga agcacgctac atcgtagacg
     3961 agaacggcaa gcgcgtgagc gtgatactgc ccgtcgagga gtacgagcgc atgatcgaag
     4021 agttggaaaa cgtagaggac gctagggcag ccgacgaggt gagagccgct gtcgcgcgtg
     4081 gtgatgacga gttcattccc tacgagcaag ctcgggaaga gacagcaagg ggacgggctg
     4141 caaatcggtg agctaccgtc ctctatacct cgaagtgtca gcaagcgcat ggagaagctt
     4201 cccgtcgaag tctacgaccg ggtggacggg gcgatactgg cgctcgccga cgatcccggg
     4261 ctgcacgaga ctcagcggcc gagacgattg gcggatcaga gtaggagact accgaatcgt
     4321 ctacggaata gacgacgagc ggcgcgtggt tgagattctg aacgttgctc accgccgaga
     4381 cgtctaccgc cgaggataat attgcagcgc ctgagggcaa cgctcgaccg catagaccgc
     4441 aaggggtacg gagcctacaa agacctctca ggctcttatg gcttcggcca tttcaccctc
     4501 tttatcgaca aggtccagcg agatcccttc gccccacctt ctcttatacg ggttcggacg
     4561 aaagagaatg ctttcgagcg tgacctgtta gacaacccgg tgcgccggat cgcattcgag
     4621 gacttcctga ccaggagcgt ggagaggggg atccgtaacg tcgtgcgtgg aaaccggggt
     4681 tccggcggga gcgggcgggt cgagatacag cggacctccc aggtggtcct cccccgcacc
     4741 tcgatggtcg tcgagcctgg ttacgtcgag gcccgcatgg ccgtaggtct ccccgcccgg
     4801 ggccggagcg tggacgcccg agcagcccga acggtgcttc tggaagagct gcctgaggtc
     4861 gtgcgacgtt cgctcgtccc gactccagaa ggtggcgttg atggggaaag aacgaggctc
     4921 cacgtcgaga ccgtcgagga cgcggaccat ctgcggcgac tcctgcctga gctgggtctg
     4981 gtcgcgttcg tcgccgacgg ggcggtgttg ccgagggaga gcggggcgag cgacctgcct
     5041 ttgagggagg gtgccgtacc gtttgcgagc ccggaggagt tcagggtgga ggtggacctc
     5101 ccgaaccggg gcatcatctc gggaatgggg attcccgagg gcgtcacgct cgtcgccggg
     5161 ggcgggtttc acgggaagag cacgctgctc tcggcgctct cctggggcgt ttacgaccac
     5221 gtgcctgggg acgggcgaga atacgtggtt gcgcgggagg acgcagtcaa ggtcagagcc
     5281 gaggacggca ggagcgtctc gggggtcgac atttcggcca tgatcgggga cctgccgggc
     5341 ggacgtacta cggaggactt ctcgacccca aacgcctctg gctcgacctc gcaggcggcg
     5401 aacatcgccg aggccatcga ggtagggacg tcgctcctcc ttgttgacga ggacacgagc
     5461 gcgacgaact tcatgataag ggacgagagg atgcgcgagc tggtgaggag agagcccatc
     5521 tcaccgttta ttgacctggt gcttccactt caccgtgcct taagggtatc gacggtcgtc
     5581 gtcgttgggg gagtgggcga ctaccttgac gttgctgacc gtgtgatcct gctcgaggac
     5641 tacgcgccct cggacgccac acgccatgcc cgtgaggtga cagcgaagtt cccgccccgc
     5701 gccccgctat cgcacagaga gatgtatccc ccgcgggagc gcatcatcca cgcatcctcg
     5761 atagacctga gacgcggcaa acgccgtacg gcccgtggca ggggcctcgg catcatcgaa
     5821 ctcggccgcg agcgggtcga cctgtcctac ctcgagcagc tcgccgagac cggccagacg
     5881 gaggcggtgg cccgtataat cggacggttc gccgccgccg gcgaggtcag ggagatgcgg
     5941 gagctcgtca gggaggcgct ggcatccgtc tccgggaacg gcctcgactc tctgggtaac
     6001 ttcgagggac accctggtga gatgagcctg ccacgcgccc aggaagtggc ggctgccata
     6061 aaccgcgtcc gcccgctatt ggcgagcccg aagatgtaga agccgacccg tgctggctgg
     6121 tatcattagg tattgcgtca ggaggagagt aggacagact ggatgaaaga aaccgccgtc
     6181 gaagacacta atgtgagcaa cacggatacg ccttcctggt tcaggctgaa agccgttgcc
     6241 gtacacctgt acacggcgag cggcgcggtg ctcgcgctcc tgatgctcgt tgcggctttc
     6301 cagggtgagg ctgtgaaggc cctctggctt atgctcgcat cgctcctcat cgacagcacg
     6361 gacggtctcc tcgcccggcg cttcagggtc agcgaggcgc tccccttctt cgacggggcg
     6421 ctcctcgaca acatcgttga ctacatgacc tacgtcttcg cccccatggt gttgctgtgg
     6481 agcggagggt atctgcctga tggcaacgca gggatagttc tcgccgcgct tccgctcctg
     6541 gcctccagct accagttctg ccgcgttgac gccaagaccg aggaccacta cttcctcggc
     6601 ttcccgagct acttcaacgt cgtcgccttc tacgccatcg tcttcgagcc aggcccgctg
     6661 gtgctcgccg ccgtgctcgt ggcctgctct ttcctggtct tcattcctat tcgctacgtc
     6721 tacccgacgc gcacggtcgc tttccggggg ctctcgctca ccctcacggc gctatggctc
     6781 gcgagctacg ccgcgatact ctggcagatg ccacatccgg atccactaat cctcgccttc
     6841 tccatcctct acctcgtcta ctatttcggc ctcagcctct acctctctgg aaagatgctc
     6901 gaagccaggc gactcgaaga ggagatcgca gactaggaag cccgccgggc tcttcatcgg
     6961 tctgtgcggc accgatcagg gaggaccacg tggccggacg cgcaagcggc cctccaaccg
     7021 tcatactacg ggacgtggct gaaggcgacc tccccgtatt cttcgaacac cagctagacc
     7081 ccgaagcgac ccgtatggcc tccttcccgg ccagggacag aaatgcattc atggcgcact
     7141 ggaccaggat tctctccgac gagactgtat tcacgaaaac catactcttc gacggacgtg
     7201 tggctggcaa cgtcgtgagc ttcgtacact caggtgaacg ggaggtgggt tactgggttg
     7261 gcaaggaatt ctggggaaag ggaattgcca cacaagcact ctcggtgttt ctggaccaga
     7321 tagagacgcg tcccctgtat gcccacgtcg cccgccacaa catcggctct atccgggtcc
     7381 tccaaaaatg cgggttcagg atctccggcg aggagcccga agggttgatc ctgcaactgg
     7441 aagcgaacga acgagacgaa gcgcgatgaa ggtatgacgg acatactcat aaccgaaagg
     7501 agcaaatacg tgacgaacga aagccatcac gaatccgcta ggcggacaca tggtagtcaa
     7561 ggtacgcgac gggataccgg cggcgtcgtc caccagccct cgaacccagg gacggagcca
     7621 accagagtat tgctcatctt ctcccccgct gggatggatc gctttttcga agaggcggcc
     7681 gagggccgca tgccacttca agcggtgcca acagatcctg aggtaaagga gagactcgat
     7741 tcttttacaa gcaggtacgg ctacgagttc gcggagttcc cgcagtagag acagaggtca
     7801 atttcttgtc acatccggcg ctttgacgcg tcgtcgcgcc gtgttaggct ccctgcgcaa
     7861 cctccatagc gaaaggaaga gcatggcgct aaacgagaca cttttgaagc ggttgatcga
     7921 gactcccgga gtttcagggc gggaggagcg gcagcgtgag atcgctcgcg aagagcttga
     7981 cactttgacg gacgaggtac gcaccgacgc cctgggaagc gtcataggga cgatgaaggg
     8041 aagcgacgac acgcgcgtga tggtcgccgc gcacacggat gagatcggct acctcgtcaa
     8101 acacatagac gacaagggct tcgtccgcct gcagaccctt ggcggccacg accccgcgaa
     8161 catggtctcc cagagggtca tcataacgac cgccgaaggc gagacgctcc gcggagcgct
     8221 ccaaccagct cgcaagcctc cgcaccttgc acacggcgag gagcagaaac ccccgaaggc
     8281 cgacgagttc tatgtcgacc tcggcatgga cgccgagacg atgaaggaga aggtgcgcat
     8341 cggggattat gctgtcatgg accgcaccct ggagaaggtc ggcgacaact acataggcaa
     8401 ggcgatggac gaccgcatcg gcgtcttcgt catgtacgag gcgctgaggg ccatgggaga
     8461 ccaccaggcg accatcttcg ccgccgcgac ctcacaggag gaggtgggac tgcgcggagc
     8521 agcggccagc ggctcagccc tccagccgac ggtcgtcgtg gcgctcgacg tgacgctcgc
     8581 gatggacgtg cccggagcgg ggaacgagaa caccataacc gagctaggaa aaggggtggc
     8641 gataaagatc atggacggct actccatctc ccacccgaag ctcgtggagc acctccgcgc
     8701 catcgccgag cgcgaagaca tcccctacca gatggagata ctccccgcag gcggcaccga
     8761 cgccggtggc gtccagcaac tgcacggcgg catccccgcc gtcacgctct ctgtcccctg
     8821 ccgctacgtc cacaccccga acgagatggt cagcgccacg gacgtcgagg ccgccattac
     8881 gctcctcgcc cgctacctcg aagaggctca ctccgcagac tactcgctct gagccggcgc
     8941 ccgacaacat acgatcgcgc tatacttgac cataatggtc aagtatagcg gttcagagga
     9001 ggttcctgat ggaatcggcg ggtgtcgcgc agttgaaagc gaggctcagc gagtatctcg
     9061 cacgagttag ggcgggtgag gaggtgctgg ttacggacag aggtcagccc gtcgcccgtc
     9121 tcgtcccggt cggctacctc ccggacgacg aagcggagct ggcgcggttg aaggctatgg
     9181 agcgggaggg tctgatgcgt ctcggctccg gcaggctgcc agcgggtttc ttcgagaagg
     9241 agcggccggc ggacccggaa gggctcttga gggaggccgt actagaggag agagaacgag
     9301 gccgctaagc gatgcggttc tgggatacat cggcgatcat gcccctcatc tcgcgcgagc
     9361 ctctctcgga ggagatgcgg cggttgctcg aagaagacgc ggggatcgta gtctggtggg
     9421 ccacccgcgt ggagtgtata tcggcgatct cgcggcgcgc cagggaaggc tcgctcggaa
     9481 ccgaaggaga agcgtcggcg cgcggcttgc tcgacgacct atcggaaagc tggaccgagg
     9541 cacggcccac gacccggcta cgtgctttgg ccgaacaagc ccttgcggcc cacccactgc
     9601 gagccgccga cgccctccag atggcctcag ccatgatctg gtgcgggtct cagccccggg
     9661 gacgcccctt cgtctgcctc gacgaacgat tgagagcagc ggcatcccgc tcaggcttcg
     9721 tcctgcttcc gaacgcacac acagattgaa acccggatac tacgcgaggg aaaggagcgt
     9781 atgggggaat ggaaggggac tcttcgacaa ccgtcatggc gcgagcgatc gaggagaacg
     9841 gcgccgagtt cctgatgtcg ctcggacgcg cggcaggagc cgagatacgc gatgacgaac
     9901 ggctgcgatg ggccatcggg aactccccca tcgattacca caactgcgtc gtccacgcct
     9961 acctgacccc ggaagaggcg gacgaggaga tcgaagtgtc cgtggaacgt atgcgggcgc
    10021 acggggttcc cggttcctgg cacgtcgggc cctcgatgcg cccgcccgat ctcggaaaac
    10081 gcctcgtcgc ccgcggcttc gagtacggcg gcgacgatat agggatggct gtggacctct
    10141 cgatgctgcc ggaggaggtg acggtaccag aagacttcct ggtcgagcgg gtgcgcgacg
    10201 aagcggggct cgcggcgtgg gtcgaggcgc tcggctcggg cttcggcgaa ggacctgtcg
    10261 aggccgagtg ggtcggagaa atgtatcgca ggctgggaga agatgcctcg ttgcggcact
    10321 atctggggcg attgggtgga gagcctgtgg ccacggccac tcttttcatt ggtgccgcgg
    10381 tcgctgggat ctacttcgtc tgtaccgtcg ggcgcgccag gcggaggggc atcggggctg
    10441 ccgtgacgct cgcgccgctt cgggaggcgc gggatctcgg ctccagtgtc ggtgtccttg
    10501 gttcttcgga gatgggttac cccgtctacc gcaggctcgg cttcgaggaa tactgcagga
    10561 tcgggctcta cgagtggcgt cccgggtagg cgatgccaaa acccaactct tgatgcggta
    10621 gcatgtgcgt tcgacgttag ggcgaacacc tatggaggga acctcgatgg cagacaagaa
    10681 ctacgacagg ccgccggaga tgacgctcga acagggctac gaatactatg ccaatttcgt
    10741 taccgagaag ggccgggtga agatcaggct gttcgcggac gaggcgcctg agacggtcaa
    10801 caacttcgtc ttcctggcgc gcgatgggta ctttgacggg acgaccttcc accgcgtcat
    10861 agagaacttc atggtccagg gtggggaccc gacgggcacg gggacgggcg gccccgggta
    10921 cacgatctct gacgagttcc atcccgagct gcgccacgac aggcccggcg tactttcgat
    10981 ggcgaacgcc ggcccgaata caggcgggtc gcagttcttc atcacgcacg tggcgacgcc
    11041 gtggctggac gacaggcacg ctgtcttcgg ggaggtcatc gaaggcatgg atgtggtgaa
    11101 cgccatcagg gagcgcgacc cgcagcgcga ccgcgagccc ggcgacaaga tagagcgggt
    11161 cgagatcgaa gaggtctccc tgtcctgatt ccacccttga gtgcgcattc acagtttgtc
    11221 ccgagggtcg cacgataccc tcgtcgggca aacgagatcg cacgagagag
//
LOCUS       DATAIR010000818         5869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_81043, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000818 DATAIR010000000
VERSION     DATAIR010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81043"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VHM69_19420"
     CDS             <1..1368
                     /locus_tag="VHM69_19420"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX2742619.1"
                     /translation="NMDSTQVQGRIDLETLKTEVEAGRIDTVLIAMTDMQGRLQGKRL
                     TASHFLDEVVEHDAEGCNYLLAVDVEMNTVEGYAMSSWERGYGDFVFKPDMDTLRYVP
                     WQEGTAFVTCDLVWEDGSPVVASPRQILKRQLKRLSERGLEAFVGTELEFLVFMDSYE
                     EAWRKGYRNLEPANYYNVDYSLLGTARIEPLLRRIRNSMAGAGLAVEDAKGECNFGQH
                     EINFKFDRALATADGHAIYKNGAKEIAAQEGCSISFMPKFDEREGNSCHIHLSLRGED
                     GTPIFADNSGFSDTFEHFIAGQLACLRELTLFFAPNINSYKRYAKGSFAPTAVAWGKD
                     NRTCSLRVVGHGNSLRVENRLPGGDVNPYLAIAAMIAAGLHGIENELSLEDEFAGNAY
                     ETDKPHVPTSLREARELFADSEAARNAFGEEVVEHYLNMARVEIEAFEAAVTDWERYR
                     NFERL"
     gene            1365..2075
                     /locus_tag="VHM69_19425"
     CDS             1365..2075
                     /locus_tag="VHM69_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2742620.1"
                     /translation="MSRPNIGITAATENVSYGVWKEIPAFMSPASYVRAVQRARGRPL
                     LLLPDPEDAEDPGGVLDLVDALIVTGGAGDVNPALYGQERHPETGPIQDERDAYELAL
                     VRKALEREVPILGICRGMEILNVVYGGGLEQHLPDVLGHEDHRHTPGTYSDHEVRLEP
                     GSLAARAAGEERSQVKSHHHQGVREIGSGLAVTGWADDDTVEALEDPSCPFVLGVLWH
                     PEEDEKSQVIKALVSEVS"
     gene            2076..3437
                     /locus_tag="VHM69_19430"
     CDS             2076..3437
                     /locus_tag="VHM69_19430"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2742621.1"
                     /translation="MLEVLNPATEEVVKRLEPATEDDADAAIVRARDAFPAWRDVQPD
                     DRARLLRRLADALESEQENLARLESQNTGKPIGDSRGEMAMVAETFHYYAGMPERLLG
                     DTIPVSGGVDVTFREPLGVVALIVPWNFPLTIASWKMAPALAAGNTLVLKPAGLTPLT
                     AMEFERIALEAGIPEGVVNVVVGPGSKVGKRLVEHPDVAKVAFTGSTEVGKGIMAGAA
                     STIKRVTLELGGKSANVVFADADLEKAAASAPVAVFGNAGQDCCARSRILVEKSVFDE
                     FLSLMQPVVEGFRVGDPFDEETEMGPLISADHRENVAAYVTEDAPVAIRGEAPEGSGF
                     WYPPTVLAPVSNEDTAAREEIFGPVVAVIPFDNEEDAIRQANDTIYGLSGSIWTQNGA
                     RALRVARRMETGVISINSNTSVRVATPFGGMKQSGVGRELGPHALDYYTEIKNVYLST
                     EED"
     gene            3439..4206
                     /locus_tag="VHM69_19435"
     CDS             3439..4206
                     /locus_tag="VHM69_19435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX2742622.1"
                     /translation="MPGRLEGKVAVITGGAGGIGRETALRFAEEDARVCIADLADEPG
                     NEVAGEVDGLYIHADVTDPDDVQKMYRETAERFGGIDVLFNNAGISPPDDDSILDTEL
                     DAWQRVQDVNLKSVFLCCKYGIPHLLERGGGSIINTASFVAVMGAATSQISYTASKGG
                     VLALSRELGVEFARQGIRVNALCPGPVNTPLLQELFSKDPEKAQRRLVHLPMGRFAEA
                     TEIASAVLFLASDDASYVNASTFLVDGGLSGAYVTPE"
     gene            4219..5154
                     /locus_tag="VHM69_19440"
     CDS             4219..5154
                     /locus_tag="VHM69_19440"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX2742623.1"
                     /translation="MKSGLSNSILDAIGNTPMLEIEGIYCKLEYLNPSGSIKARIAKY
                     MVERAEDEGLLQQGDTIVEATSGNTGNALSMVAAVKGYRMLVVMPEGMSGERVAISRA
                     FGAEVMEVGHFHVNEALAKAKELGSEPGYFCPSQFESEWNVEENRIWLGPEILNQLPE
                     GVVPDAMVMGVGTGGTLIGVGQAFREVNPNVKLFAMEPSESSTILCGEVGDHLIEGIA
                     DGFVPGIFERHQQLVDTTLAVDSEEAVETMRRLARQYGLFVGPSSGANLIAAKKVREA
                     YPELETVVTLFCDEGEKYISEHFADGVRITSETYT"
     regulatory      5340..5459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5572..>5869
                     /locus_tag="VHM69_19445"
     CDS             5572..>5869
                     /locus_tag="VHM69_19445"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742624.1"
                     /translation="MARFTPHTEADIQEMLEAIGVETLDDLFADVEPKYEGELDLPPA
                     LSEYESLREVDALSKENVSGRPIFLGAGAYDRIVPSAVGTIISRGEFLTCYTPYQ"
BASE COUNT         1217 a         1834 c         1864 g          954 t
ORIGIN      
        1 aacatggact cgacgcaagt ccagggacgc atcgatctcg aaacactcaa gaccgaggtc
       61 gaggcaggaa ggatagacac cgtactcatc gcgatgacgg atatgcaggg ccgcctgcag
      121 ggcaaacgcc tcaccgcgag ccacttcctc gacgaggtgg tcgaacacga cgcagaaggc
      181 tgcaattacc tgctggctgt ggacgtggag atgaacaccg tcgaaggcta cgcgatgtcc
      241 tcatgggagc gcggctacgg cgacttcgtg ttcaagccgg acatggacac cctgagatac
      301 gtaccctggc aggagggtac ggcgttcgtc acgtgcgacc tcgtctggga ggacggctcg
      361 ccggtcgtgg cctcgccgcg ccagatcctc aagcgccagc tgaagaggct ctccgagcgc
      421 gggctcgaag ccttcgtggg aactgagctg gagttcctcg tctttatgga ctcctacgag
      481 gaggcctggc gcaaggggta tcggaacctg gagcctgcca actactacaa cgtggactac
      541 tcgctcctcg ggacggcgcg catcgagccc ttgctgcggc gcatacgaaa ctcgatggcg
      601 ggggccggtc tcgcggtcga ggacgccaag ggcgaatgca acttcgggca acacgagatc
      661 aacttcaagt tcgatcgggc gctcgccacc gccgacgggc acgcgatcta caagaacgga
      721 gccaaggaga ttgcagctca agaaggatgc tcgatctcgt ttatgccgaa gttcgacgaa
      781 cgcgaaggca actcctgcca cattcacctg agcctgcgcg gggaggatgg cacaccgatt
      841 ttcgccgata acagcggctt ctcggacacc ttcgagcact tcatcgcagg ccagctggcc
      901 tgcctgcgcg agctcacgct cttcttcgcc ccgaacataa actcatataa gcgctacgcg
      961 aagggctcct tcgccccgac ggcggtggcg tggggcaaag acaaccgtac ctgctccctg
     1021 cgcgtcgtcg gccacggcaa ctctttgcga gtagagaacc ggctgccagg cggagacgtg
     1081 aacccgtacc tcgccatcgc ggccatgatc gccgccggtt tgcacggcat cgagaacgaa
     1141 ctctccctgg aagacgagtt cgccggcaac gcctatgaga cggacaagcc ccacgtaccg
     1201 accagtcttc gcgaggctcg ggaactgttc gcggacagcg aggcggcacg caacgcattc
     1261 ggcgaggaag tcgtcgaaca ctacctcaac atggcgaggg tcgagatcga agccttcgag
     1321 gccgccgtca cggactggga gcgctacagg aacttcgagc gtctgtgagc cgcccaaata
     1381 tcggcataac cgccgcgacc gagaatgtta gctacggtgt ctggaaggag atacctgcct
     1441 tcatgagtcc ggcgagctac gtgcgagccg tgcagcgggc gagagggcga cctcttctcc
     1501 tgctccccga cccggaagac gccgaagacc ctggtggggt tctggacctg gtagacgcgc
     1561 tcatcgtcac ggggggagct ggggacgtga atccagccct ctacgggcag gagcggcacc
     1621 ctgagacagg cccgatccag gacgagcgcg acgcctacga gctcgccctg gtgcgcaagg
     1681 cattggaacg cgaagtgccc atccttggca tctgccgcgg gatggagatc ctgaacgtcg
     1741 tctacggcgg cggcctcgag caacacctgc ccgacgtgtt gggacacgaa gaccatcgcc
     1801 acacccccgg tacctactcc gaccacgagg tgcgtctcga gcccggctct ctcgccgcgc
     1861 gggccgcagg tgaagagagg tcgcaggtca agtcgcacca ccatcagggg gtgagggaga
     1921 tcggtagcgg cctcgccgtg acgggctggg ccgacgacga cacggtcgag gccctggaag
     1981 acccgtcctg cccgttcgtg ctaggcgtac tctggcaccc tgaggaagat gaaaagagcc
     2041 aggtcataaa agcgctagtt tcggaggtct cttagatgct cgaagtcctg aatccagcga
     2101 ccgaagaggt ggtgaagagg ctcgaacccg ccaccgaaga cgacgccgac gcggccatcg
     2161 tccgcgccag ggacgccttc ccggcgtgga gggacgtaca acccgacgac cgtgcgcgtc
     2221 tcctcaggcg cctcgccgac gccctcgaat cggaacagga gaaccttgcg cggctggagt
     2281 cgcagaacac gggcaagccc ataggcgatt cccgcggcga gatggcgatg gtcgctgaga
     2341 ctttccacta ctacgccggg atgccggagc gactgctcgg cgatacgatc cccgtctccg
     2401 gcggcgttga cgtaacgttc cgcgaaccgc tcggcgtcgt cgcgctcatc gtcccatgga
     2461 acttcccgct caccatagct agctggaaga tggcccctgc gctcgccgcg ggcaacacgc
     2521 tcgtcctcaa gcccgccggg cttacgccac tgaccgccat ggagttcgag cgcatagcgc
     2581 tggaggccgg catccccgag ggcgtcgtaa acgtcgtcgt cggcccagga agcaaggtgg
     2641 ggaagcggct cgtcgagcac cccgacgtcg cgaaggtcgc tttcacgggc tcaacggaag
     2701 ttggcaaggg gatcatggcc ggcgccgcaa gcaccataaa gcgtgtcacc ctggagctcg
     2761 gcggcaagtc cgcgaacgtg gtcttcgccg acgccgacct ggagaaggct gctgcctcag
     2821 cgcccgttgc cgtcttcggc aacgccgggc aggactgctg cgcccgctcc cgcatcctcg
     2881 tagagaagag tgtcttcgac gagttcctct cgctcatgca gcctgtggta gagggtttcc
     2941 gggtcgggga cccgttcgac gaagaaaccg agatgggacc cctgatctcc gccgaccacc
     3001 gcgagaacgt cgccgcatac gtcaccgaag acgcaccagt ggcgatacgg ggcgaggcgc
     3061 cggagggaag cggcttctgg tacccgccca ccgtactcgc ccctgtatca aacgaagaca
     3121 cggcggctag agaggagatc ttcggtcccg tcgtcgcggt gatcccgttc gataacgaag
     3181 aggacgccat caggcaggcc aacgacacga tctacggcct ctcaggttcg atctggacgc
     3241 agaacggcgc ccgcgccctg cgcgtggccc gccgcatgga gacgggcgtg atctccataa
     3301 actcgaacac ctctgtcagg gtcgcgaccc cgttcggcgg gatgaagcag tcaggcgtcg
     3361 ggcgcgagct cggcccgcac gccctcgatt actacaccga gataaagaac gtctacctgt
     3421 caacggagga ggattagagt gcctggacgg ctcgaaggga aggtcgccgt cataaccggc
     3481 ggcgcaggcg gtataggacg tgagacggcc ctgcgcttcg ccgaagagga tgcccgcgtc
     3541 tgtatagcag acctcgccga cgaacctggc aacgaggtcg ccggcgaggt ggacggcctc
     3601 tacatacacg ccgacgtcac cgaccccgat gacgtgcaaa agatgtaccg cgagacggcg
     3661 gagcgtttcg gcggcataga cgtgctcttc aacaacgcag gcatctcgcc gcctgatgac
     3721 gactctatcc tcgacacaga actcgacgcc tggcagcgcg tgcaggacgt gaatctaaaa
     3781 tctgtcttcc tgtgttgcaa atacggcatc ccgcacttgc tggagcgggg cgggggttcg
     3841 atcatcaaca cagcatcctt cgtggcggtg atgggggcag cgacgtccca gatctcctac
     3901 acagcatcga aaggaggcgt gctcgcgctc agccgcgagc tgggagtcga gttcgccagg
     3961 caggggatac gcgtcaacgc gctctgtcct ggacccgtga acacgccgct cttacaagag
     4021 ctgttctcca aagaccccga gaaagcgcag cgcaggctcg ttcacctgcc gatgggccgg
     4081 ttcgccgagg cgacggagat cgccagcgcc gtgctcttcc tagcctccga cgacgcctcc
     4141 tacgtcaacg cctccacctt cctcgttgac ggcggcctct caggggcgta cgttacccct
     4201 gagtaggagg tggtcggcat gaaaagcggt ctatccaaca gcatactcga cgccataggg
     4261 aacacaccga tgctcgaaat cgagggcatc tactgcaagc tggagtacct gaacccgtca
     4321 gggtccatca aggcccgcat cgcaaagtac atggtcgaac gcgccgaaga cgaagggctg
     4381 ctgcaacaag gcgacaccat cgtcgaggct acgagtggca acaccggcaa cgccctctcg
     4441 atggtcgccg ctgtcaaagg ctacagaatg ctcgtcgtaa tgccggaggg gatgagcggc
     4501 gagcgggtgg ccatcagcag ggccttcggg gctgaggtaa tggaggtcgg ccacttccac
     4561 gtcaacgagg ccctcgcgaa agccaaggag cttggaagcg agcctggcta cttctgtccc
     4621 tcccagttcg agagcgagtg gaacgtagag gagaacagga tctggctcgg acccgagatc
     4681 ctgaaccaat tgccggaggg cgtcgtgcca gacgcgatgg tcatgggagt agggaccgga
     4741 gggaccctga tcggggtcgg ccaggctttt cgcgaggtga atccgaacgt caagctcttc
     4801 gcgatggaac cctccgagtc atcgacgatc ctgtgcggag aggtcgggga tcacctcatc
     4861 gagggcatcg ccgacggctt cgtccctgga atcttcgagc gccaccagca actcgttgac
     4921 accacccttg ccgtcgactc cgaagaagcc gtcgagacca tgcgccgcct cgcgcgccaa
     4981 tacggtctct tcgtcggccc ttccagcggc gccaacctca tcgccgcgaa gaaggtgagg
     5041 gaagcctatc ccgagctaga aaccgtcgtc accctgttct gcgacgaggg cgagaagtat
     5101 ataagcgagc acttcgccga cggcgtacgg atcacctcgg agacttacac ctgagccata
     5161 aagtccccag gagcacacgt tcggaaaagg cactctgggc ggatgggtaa tttccgtgat
     5221 tgggttgctg ctaatttgct gccaattgct gctaaattag caaccaacga cgattgctaa
     5281 acgatgtacg cggcttcgca agtcgcgcgc cggcacttat gctaacttta ccttgcccga
     5341 tgagggccgc gggagagagt cgtctgaggg atgggggaga cctcgtggcg gccgccgaag
     5401 gtgaaaggtc cgtggagtca cgggccgaat ctctcaggca gaaggaccgc gtgccggagg
     5461 gcgtcctgga gagttcggtc cagcgctgga tcggcgccga cggggtaaca ctctcaggtt
     5521 tgggacaggg cggaaaacga gaacgctcac ccgacaaggg ggtatagccg cgtggcgcga
     5581 ttcacaccgc acacagaggc cgatatacag gagatgctgg aggccatcgg ggtcgagacc
     5641 ctggacgacc tcttcgccga cgtcgagccg aagtacgagg gggagctgga tcttcctcca
     5701 gccctctctg aatacgagtc gctacgagag gtagacgctt tatctaaaga gaacgtctcg
     5761 gggcgcccga tcttcctcgg agccggagcc tacgacagaa tagtaccttc agcagtaggc
     5821 accatcatct cgcgcggcga gttcctgacc tgctacacgc cgtaccagc
//
LOCUS       DATAIR010000819         5655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_276355, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000819 DATAIR010000000
VERSION     DATAIR010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276355"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VHM69_19450"
     CDS             <1..542
                     /locus_tag="VHM69_19450"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX2742625.1"
                     /translation="TSLEPHQLLETMSLAVDFMRNRSLVELFEVRRMIEPAATALAAG
                     RITTEDLLRLDETLRRMDSAGSVEELVEADDDFHRTIAGATGNAALAAFLQNLYGRTL
                     RARIWRGLAEEGALDRTRVSHHAIYTAISNRDAELARSAATMHIAEVEAWFRRLPEIR
                     EAAPPNGNDGSDYPGHAKP"
     gene            565..1635
                     /locus_tag="VHM69_19455"
     CDS             565..1635
                     /locus_tag="VHM69_19455"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX2742626.1"
                     /translation="MSGYGDFGSSLRDLPRLRSSRRRRASSWDRSGGNDDRLTIEPGA
                     SAVLVQIQGAGCVNHVWITCAIEGPETKEPDFLRRLLLKMYWDGEEEPSVLVPLGDFF
                     GVGHARTTNFVSSPLQMSPEDGKSFNCFFHMPFASDARIEVVSELEHEKVFFYYYVDY
                     EEFDDLEEVLGRFHAQWRRENPTDGVEEGDQSNEEFLFGGENASGEGNYTILEAEGRG
                     HYVGCVLNVQNLRETERWNWYGEGDDMIFIDGEEWPPFLHGTGTEDYFNTAWCPREKY
                     SAPYHGITLGGGENWSGHASLYRFHVEDPVTFERSIRVTIEHGHANKRSDDFSSVAYW
                     YQTEPHKPFSVLPVEQRLPRPI"
     gene            1796..3292
                     /locus_tag="VHM69_19460"
     CDS             1796..3292
                     /locus_tag="VHM69_19460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2742627.1"
                     /translation="MSDLLLGVDIGTSSTKGVLARPDGEVVATAERPHELSLPRPGWA
                     EHDSEKVWWDDFVSVCRELLEKADDSIAAVCASGIGACLLPSDEGGVPLRPAILYGID
                     TRAEREVEELNDRYGREALLERCGSVVTTQAVGPKLLWLRRNEPEVWEKTRKFFMAST
                     FVAYRLTGEYVLDHHSASQCDPLYDVHEYGWIEDRGEEIAPNLPLPRLFWPSEVVGEV
                     SREGAEATGIPAGTPVAAGTIDAWSEGASVGVQEPGDLMLMYGTTMFMIEVLKEPLHH
                     PGLWGTTGILPRTHNLAAGMATSGALTGWLKKLSGDLPYEELTREAAAVPPGSDALVV
                     LPYFAGERTPIFDPKARGLISGLTMSHGRGHLYRAMLEATAYGVRHIFEYMREAGGGG
                     ERLVAVGGGTKGGLWTQIVSDVTGRPQDLPEQTIGAAYGDALLAARAVGLVEPDIDWS
                     TISNTVEPDEENRGIYDELYGIYRELYPATREASHRLADLQKGADVVT"
     gene            complement(3306..4592)
                     /locus_tag="VHM69_19465"
     CDS             complement(3306..4592)
                     /locus_tag="VHM69_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX2742628.1"
                     /translation="MQTENPVYEEIEEILRTVEFPRVALVEQRVDSPPALTDIREGVR
                     EALRSVDLPSGSVAIGVGSRGVARIGEVVAALVEALKESGAEPFVVPAMGSHGASTAE
                     GQAQVLAHLGVSEESVGCPVRATMETVRLGETPSGVAVYMDRNAYEADSVVVVNRVKP
                     HTAFRGAVESGPTKMLAIGLGKQRGAHSIHSAGWENIHRTIPEAARVAVESGKVAFGL
                     ATVENADEEPCKIAAIPAQELEEAEAPLLEEAKKNLARLPFDDIDVLVVDEIGKNISG
                     DGADPNVTGRYPTTAASGGPSVERMVYLGLTEETGGNANGLGMADVVTERLAGAMDRP
                     STYMNALTSTTPAPVKTPMVMPTDEMTIAAALTMCAGVTYPEARLVRIENTLKLRRIW
                     VSEALIGEVESDERLEILEEPRPMRFDSGEITPRSA"
     gene            5137..>5655
                     /locus_tag="VHM69_19470"
     CDS             5137..>5655
                     /locus_tag="VHM69_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2742629.1"
                     /translation="MERSWNLRVALFIPCYVDQINPEVGVSVVRVLRRLGVDVIYPEG
                     QTCCGQPAFNSGFFDEARTIGRRFLDVFENETFDYIVCPSGSCTTMVSHYYPFIFKEL
                     PEERERSEVIGERVREFSDFLVNVIGAKDLGARLEGKAVFHCGCHQRRELGVLEEPRE
                     LLGNVDGLDLVEW"
BASE COUNT         1024 a         1808 c         1812 g         1011 t
ORIGIN      
        1 ttactagcct ggaaccccac cagctcctcg agacgatgag cctggcggtg gacttcatgc
       61 ggaaccgctc cctcgttgag ctcttcgagg tgcggcgaat gatcgaaccc gccgccaccg
      121 cgctggccgc aggccgcatc actacggaag atctcctgcg attagatgag accctgcggc
      181 gcatggactc agcagggagc gtcgaggagc tggtcgaggc cgacgacgac ttccaccgaa
      241 cgatcgctgg cgccacgggt aacgccgccc tggccgcgtt ccttcagaac ctctacggca
      301 ggaccctgcg ggcccgcatc tggcgcggcc tcgcagagga gggtgccctc gaccgtacca
      361 gggtctcgca ccacgccatc tacacggcca tctcgaacag ggatgccgaa ctcgcgcggt
      421 ccgccgccac catgcacata gccgaggtcg aagcgtggtt ccgccgcctg ccggagatca
      481 gggaggccgc tccaccgaac gggaatgatg gatccgacta tcccggccac gcgaaaccgt
      541 aggtagaccg gaaaggagcg aagcgtgagt ggctatggtg acttcgggag cagcctgcgc
      601 gatctgccga ggctgagatc cagccgtcgc agacgggcgt cgagctggga ccgctcgggc
      661 ggcaacgacg accgactcac catagagcca ggcgcgtcgg cggtactggt gcagatacag
      721 ggcgcgggtt gcgtgaacca cgtctggatc acctgcgcca tcgaaggccc cgagacgaag
      781 gaaccggact tcctgcgccg gctcctcctc aagatgtact gggacggcga ggaggaaccg
      841 agcgtgctcg tgcctcttgg ggacttcttc ggcgtaggcc acgcacgcac gaccaacttc
      901 gtctcatcac ccttgcagat gagcccggaa gacggcaaat ccttcaactg cttcttccac
      961 atgcctttcg cttccgacgc caggatagag gtcgtgagcg agctcgagca cgagaaggtc
     1021 ttcttctact actatgtcga ctacgaggag ttcgacgatc tcgaagaagt actcgggcgc
     1081 ttccacgccc agtggcgcag ggagaaccca acggacggcg tcgaggaggg ggatcagagc
     1141 aacgaggagt ttctgttcgg cggtgagaac gcttcgggtg aggggaacta cacgatcctt
     1201 gaagccgaag ggcgtggcca ctacgtcggc tgcgtgctga acgtccagaa cctgcgcgag
     1261 acggagcggt ggaactggta cggtgagggt gacgacatga tctttatcga cggcgaggag
     1321 tggccgccct tcctgcacgg cacgggcacc gaggactact tcaacaccgc ctggtgcccg
     1381 cgggaaaaat actctgcgcc ctaccacggc atcaccctgg gcggtgggga gaactggagc
     1441 ggccacgcca gcctctaccg cttccacgtc gaggaccctg tgacgttcga acgttccatc
     1501 cgggtcacca tcgagcacgg ccacgccaac aagcgcagcg acgacttttc gtcggtagcc
     1561 tactggtacc agaccgagcc gcacaagccc ttctccgtcc tgcccgtcga acaacgtttg
     1621 ccccggccaa tatgatcccc gcgtataagg tcgtgaagcc acacgttgcg caagggcggg
     1681 tcgtgtggaa ccatgcgaaa ggtttgcggg gaggagtgag gttggcacga aaagatgttc
     1741 tggcgttccc ggacggtcgg gatttggttg acgggcacgg cttcggaggc gagcgatgag
     1801 cgatttactc ctcggcgtgg acatcggaac atcgagcacg aagggtgtcc tggcccgccc
     1861 cgacggcgag gtcgtcgcga ccgcagagag gccgcacgag ctctctctac cccgccctgg
     1921 ctgggccgag cacgactccg agaaggtgtg gtgggatgac ttcgtctccg tatgcaggga
     1981 gcttctggag aaggccgacg atagcatcgc cgctgtctgc gcgagcggca tcggggcctg
     2041 tcttcttccc tctgacgagg gcggcgttcc gttgcggcct gccatcctct acgggataga
     2101 cacgcgggcg gagcgcgagg tcgaagagct aaacgaccgt tacgggcgcg aggcgctgct
     2161 ggaacggtgc ggctccgttg tgacgaccca ggccgttgga cccaagctcc tctggctacg
     2221 gcgcaacgag cctgaggtct gggagaagac gcggaaattc ttcatggcga gcactttcgt
     2281 cgcgtaccgg ctcaccgggg agtacgtact cgaccatcac tccgcgagcc agtgcgaccc
     2341 gctctacgac gtgcatgagt acggctggat cgaagaccgt ggcgaggaga tagccccgaa
     2401 cctgccgctg ccgcgcctgt tctggccatc agaggtcgtc ggagaggtga gcagggaggg
     2461 ggccgaagcg accggcatcc cggcggggac tcccgtcgca gcagggacga tagacgcctg
     2521 gagcgagggc gcgagcgtcg gggtgcagga gcccggcgac ctcatgctca tgtacggcac
     2581 cacgatgttc atgatcgagg ttctgaagga acctctccat catcccggcc tctggggcac
     2641 gaccggcatc ctgcctcgga cgcacaacct tgcagcgggc atggcgacct cgggcgccct
     2701 cacaggctgg ctgaagaagc tttcaggcga cctcccttac gaggagctga cgagagaggc
     2761 cgctgccgta ccgccaggct cggatgcgct cgtcgtattg ccttacttcg cgggcgagcg
     2821 gacgcccatc ttcgacccga aggcccgcgg cctcatctcg ggcctcacca tgagccacgg
     2881 ccgcggccac ctgtaccgcg ccatgctcga agccacggcc tacggagtca ggcatatctt
     2941 cgagtacatg cgcgaggccg gcggcggggg agagaggctc gtcgctgttg gtggcgggac
     3001 gaagggcggt ctctggacgc agatagtctc ggacgtaacg ggcaggccgc aggacctccc
     3061 cgaacagacc atcggggctg catacgggga tgccctactc gccgcgaggg ccgtcggcct
     3121 cgtcgaaccg gacatagatt ggagcaccat ctccaatact gtcgagccgg acgaggagaa
     3181 ccgcgggatc tacgacgagc tgtatgggat ctaccgtgaa ctctatccgg ctacccgcga
     3241 ggcgtcgcac aggctggccg atctgcagaa gggtgcagac gtcgtcacct gagaagggtc
     3301 agcagctatg cgctcctggg agtgatctcg ccagagtcga agcgcatcgg gcgcggttcc
     3361 tccaggatct ctaaccgctc gtcgctctcc acctctccta tcagcgcctc ggagacccag
     3421 atcctcctga gcttgagggt gttctctatg cgcacgagac gggcctcggg ataggtgacg
     3481 cccgcgcaca tggtcagggc cgcggctatc gtcatctcgt ccgtcggcat gaccatgggc
     3541 gtctttaccg gcgccggcgt ggtcgaggtg agggcgttca tgtaggtcga gggacggtcc
     3601 atcgcaccgg cgagacgctc ggtcacgacg tcggccattc cgaggccgtt ggcgttgccg
     3661 ccggtctcct ctgtgagacc aagatagacc atgcgctcca cggatgggcc gcctgaggcc
     3721 gcagtcgtcg ggtagcggcc agtgacgttg gggtctgcgc cgtcgcccga aatgttcttc
     3781 ccgatctcgt ccactacaag tacgtcgatg tcgtcgaaag gaaggcgagc caggttcttc
     3841 ttggcctctt cgaggagcgg ggcctccgcc tcctccaact cttgagccgg gatcgcggct
     3901 atcttgcacg gctcttcgtc tgcgttctcg acggtggcga ggccgaaggc gaccttgccg
     3961 ctctcgacgg cgacgcgggc cgcctcgggg atggtgcggt ggatgttctc ccagcccgcg
     4021 gagtggatgg aatgcgcccc ccgctgcttg cccagcccga tggctagcat cttcgtcggc
     4081 ccgctctcga cggctccccg aaaagcggtg tgcggtttga ccctgttcac gacgacgacc
     4141 gagtcggcct cgtaggcgtt cctgtccatg tacacggcaa cgccagacgg tgtctccccg
     4201 agcctcacgg tctccatcgt cgcccgcacc ggacacccga cgctctcctc gctcaccccg
     4261 aggtgcgcga ggacctgcgc ctggccttcg gctgtcgagg cgccgtggct acccatcgcg
     4321 gggaccacga agggctctgc gccggactcc ttcagagctt cgacgagtgc ggcgacgacc
     4381 tccccgatcc tcgctacccc ccggctcccg accccgatcg cgacagagcc ggacggcaga
     4441 tcgacagatc tcaacgcctc acgcacgccc tccctgatgt ccgtgagggc cggtggggaa
     4501 tccacccgct gctcgaccag cgcgacgcgg gggaactcga ccgtgcgcag gatctcctct
     4561 atctcctcgt agacgggatt ctctgtctgc atcccctcac ctccgtggga ccgtacttac
     4621 aactacgctt tgatctctat cctgatgagc cgtctcgttc gaaccggggc aaaccgtcgt
     4681 tcacgctctc ccaagcaact ttgtcttcca gccagatatg gcgtgtggga gggaactcgt
     4741 ccggtaggtc caggctcgcc gtggtcacat cgatggaatc aggctcttcg tcgcgcgtgt
     4801 aggtgacata cgtaccgcaa tgttcacaga aggtcctgct caccccggga gaagaacggt
     4861 actcccgtgg gtcaccggag acgaaggaga acccatcgaa agggaaagtg agccatgcga
     4921 ctgattgtgc gcctgacgcc ttgcggcacg attcgcagtg gcagatcgcc gaccccagag
     4981 gctcaccgga taccccgaat ctcaccttgc cgcaaaaaca cccgccctcg cggggataat
     5041 cgctcattcc ttctcccctt ttgttacccg cgaccattct aacagcgctc cgcccggttc
     5101 ccctctgttg ctatacttcg cgtgggaagc tctacggtgg aaaggtcgtg gaacttgagg
     5161 gtggctctgt tcataccgtg ttatgtagac cagatcaacc ccgaggtcgg cgtcagcgtg
     5221 gtgcgcgtgt tgcgcaggct tggggtggat gtgatctacc ctgaaggcca gacctgctgc
     5281 ggccagcctg ccttcaactc gggtttcttc gacgaggccc gcacgatagg tcgccgcttc
     5341 ctcgacgtct tcgagaatga gaccttcgac tatatcgtct gtccttccgg ctcgtgcacc
     5401 acgatggtct cccactacta cccctttatc ttcaaggagc tgccggaaga acgcgagcgc
     5461 tcggaggtca tcggggagcg agtgagggag ttctctgact tcctcgtgaa cgtgattggc
     5521 gcgaaggacc tcggagcaag actcgaaggg aaggccgtct tccactgcgg ctgccaccag
     5581 cggcgcgagc tcggtgttct ggaggaacca cgcgagcttt tggggaacgt ggatggcctc
     5641 gaccttgttg agtgg
//
LOCUS       DATAIR010000820         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_173362, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000820 DATAIR010000000
VERSION     DATAIR010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173362"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VHM69_19475"
     CDS             <1..487
                     /locus_tag="VHM69_19475"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742630.1"
                     /translation="WFDFGRTGGYLRHCLNLLEEPPDEEPLPTERTHEYGSYIIEAME
                     TDDPIKVNGNVPNRGLIDSLPHGCCVEVPCLVDANGVQPTTIEHYPPQLAALNRTNIN
                     VQELTVEAALSGDVEAVHHAVMLDPLTAAVCTLPEIRSMVDEMLVAQARWLPQFSEIK
                     V"
     gene            494..>1908
                     /locus_tag="VHM69_19480"
     CDS             494..>1908
                     /locus_tag="VHM69_19480"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEX2742631.1"
                     /translation="MDTTIVTDKGYEVRLDGGGEGFRGSLSTSTLESGVELVQIRIEA
                     DEAGRPPVFRLSWSYPLVSVHGFWHPGAGYDKGLGVDWGKGVYSKATSGAPVGCMYDL
                     QGRNRLTFAFSDALNPVTFHAGVHEESAEVNCWVRLFDEPLAPLTVYEATLRLDDRDL
                     PYYETLSDVSRWWARLPGYGPAGVPEAARLPMYSTWYSFHQDLDPGRVEEQCRIAKEL
                     GCEAVIVDDGWQTTSNERGYAYTGDWEPTPEKVPDMRAHVDRVHALGMKFLLWYSVPF
                     VGVHSRAWERFSGMLLEEIERLGAGVLDPRFPEVREFLIGTYEDALREWDLDGFKLDF
                     VDSFGASEEIGGGRDYDGVPEAVDRLLDDTIRRLRRIKPDVMVEFRQSYVGPLMRKYG
                     NMFRAMDCPNDAVENRMHTLDIRLLAGDTSTHSDMLMWHPEDPVHSAALQLVNVLFSV
                     PQISVLLDRIPPEHIEMLRFWL"
BASE COUNT          353 a          586 c          614 g          355 t
ORIGIN      
        1 ctggttcgac ttcggccgca ccggtggcta ccttcgccac tgcctgaacc tgctcgagga
       61 gccgcccgac gaggagcctc tgcccaccga acgcacccac gagtacggct cctatatcat
      121 cgaggcgatg gagaccgacg accccatcaa agtcaacggc aacgtgccga atcgtgggct
      181 catagatagt ctgccgcacg ggtgctgcgt cgaagttcct tgcctggtgg acgcgaacgg
      241 cgtccagcca acgactatcg agcactatcc tccacagctc gccgccctca accgtaccaa
      301 catcaacgtg caggagttga ccgtcgaggc agctctgtcg ggcgacgttg aggcggtgca
      361 ccacgcggta atgctcgatc cgctgacggc ggccgtctgt acgctgcccg agatccgctc
      421 tatggtcgac gagatgcttg tagcccaggc gaggtggctg ccgcagtttt cggagatcaa
      481 agtctaactt cacatggata ccacgatcgt gacagacaag ggctatgagg tgaggcttga
      541 cgggggaggg gagggattcc ggggctccct ctcgacctcg acgctggaga gtggtgtgga
      601 actggtgcaa atacggatcg aagccgacga ggccggacgc ccgccggtgt ttcgcctctc
      661 gtggtcatac cccctggtga gcgtgcacgg cttctggcat ccgggcgcgg gttacgacaa
      721 gggacttggg gtggattggg ggaagggcgt ctattcgaag gcgacctctg gtgcgcctgt
      781 cggctgcatg tacgacctgc aaggacgcaa tcggctgacg ttcgccttct cagacgcgct
      841 caacccggtc acattccacg ccggggtcca cgaagagagc gccgaggtga attgctgggt
      901 ccgtcttttc gatgaacccc tggcacctct gaccgtgtac gaggctacgc tcaggctgga
      961 cgacagggat ctcccttact acgagaccct ctccgacgtg agccgctggt gggcgcgact
     1021 accgggctac gggcctgccg gtgttcccga ggcggcgcgg ttgccgatgt actcgacctg
     1081 gtacagcttc catcaggacc tcgaccccgg acgtgtcgag gagcagtgcc gcatcgcgaa
     1141 ggagctgggc tgcgaagcgg tgatcgtaga cgacggctgg cagaccacga gcaatgagcg
     1201 gggatacgcc tacaccggcg actgggagcc gacgccagag aaggtccccg acatgcgcgc
     1261 gcacgtggac agggtacacg ccctcgggat gaagttcctc ctctggtact ccgtaccctt
     1321 tgttggcgtt cacagcaggg cgtgggagcg gttctcgggg atgttgcttg aagagatcga
     1381 gcgcctcggc gccggtgtcc tggatccgcg cttccccgag gtgcgcgagt tcctgatcgg
     1441 cacctacgag gacgccttga gagagtggga cctcgacggc ttcaagctgg atttcgttga
     1501 cagcttcggc gcttctgaag agatcggcgg cgggcgcgac tacgacggcg tgcccgaggc
     1561 agtggacagg cttctcgatg atacgatccg gcgcctgcgc cggataaagc ctgacgtgat
     1621 ggtggagttc cgccagagct atgtcgggcc tcttatgcgc aagtacggca acatgttccg
     1681 tgcgatggac tgcccgaacg acgccgtcga aaacaggatg cacactctgg atatcaggtt
     1741 gcttgccggg gatacgtcca cccactccga catgctcatg tggcaccccg aagatcccgt
     1801 acacagcgcg gcgcttcagc tcgtgaacgt cctcttcagc gtgccccaga tctcggtcct
     1861 cctcgaccgg ataccgcccg aacacatcga aatgctgcgc ttctggct
//
LOCUS       DATAIR010000821         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_81859, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000821 DATAIR010000000
VERSION     DATAIR010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81859"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="cimA"
                     /locus_tag="VHM69_19485"
     CDS             <1..1193
                     /gene="cimA"
                     /locus_tag="VHM69_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX2742632.1"
                     /translation="LESVRDTVSYLADAGKEVIFDAEHYFDGFRDDPAYSLAVLRAAA
                     EAGATTLVLCDTNGGTLPTELKAIVSRTVREFPDVEVGIHTHNDSGCGVANALVAIEA
                     GATHVQGTINGVGERCGNCDLVTTIANLQLKMGLKVMDDEKLRRLTEVSNYVSEIMNL
                     TPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVAPEAVGNVRRTPVGELSGKNSIR
                     AKAEELGLEAGDATEILGAIKQREYEGYHYEAADASLALLIGRTTGEDRPLFELETFR
                     IISEKRADGETTTEATIKLSVGGHRVISTAEGNGPVNALDQALRKAIGPHYPELKEIH
                     LSNYKVRILDEHRATAATTRVLIDSTDGKRMWGAVGVSENIIEASWQALVDGLEYGVN
                     GKKV"
     gene            1234..>1898
                     /locus_tag="VHM69_19490"
     CDS             1234..>1898
                     /locus_tag="VHM69_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2742633.1"
                     /translation="MSIRTLVNPNALETLDGPTPDRAPLRFHRRLPGYEATPLVDAPG
                     LAEALGVGKVFVKDESSRLGLPAFKVLGASWAVYRALEERLEDESFGDWEEIEDLKEK
                     LSPLRQLSLVAATDGNHGRALAWVARLLGFGAKIFVPQDMAAARREAIVHEGAEVIVV
                     DGTYDDAVEISAEERGLLISDMSWPGYERIPSWVIEGYSTMLWEIDDELEKRNEAGPD
                     LVVV"
BASE COUNT          384 a          571 c          652 g          291 t
ORIGIN      
        1 acctcgagtc cgtgagggac acggtctcgt acctggctga cgctggcaaa gaggtcatct
       61 tcgacgccga acactacttc gacggcttcc gcgacgaccc tgcgtattcg cttgcggtgc
      121 tgcgggcagc ggcggaggca ggggcgacga cgctcgtcct ctgcgacacg aacggcggga
      181 cattgcccac ggagctgaaa gcgatcgttt cgcgcacggt gcgggagttc ccagacgtcg
      241 aggtcggcat ccacactcac aacgactctg ggtgcggggt ggcgaacgcc ctggttgcga
      301 tagaagcagg tgcgacgcac gtacagggga cgatcaacgg cgtaggggag cggtgcggca
      361 actgcgatct cgtcacaacc atcgccaacc tgcagctcaa gatgggcctg aaggtcatgg
      421 acgacgagaa gttgcggcgg ctcaccgaag tctccaatta cgtatcggag atcatgaacc
      481 tcacgcctga cacccatcgt ccctacgtag ggcgcagcgc cttcgcacac aagggcgggc
      541 tgcacgtgga cggcatctcg aaggaccctg cgacctacga gcacgtcgcg cccgaggccg
      601 tcgggaacgt gcgccgtacg cccgtcgggg agctctctgg gaagaactcc atcagggcca
      661 aggccgagga gctggggctc gaggcagggg atgcgacgga gatcctgggc gccataaaac
      721 agcgcgagta cgagggctac cactacgagg cagcggacgc ttcgctcgcg ctgctcatcg
      781 ggaggacgac cggggaggac aggccgctat tcgagctgga gacgttcagg atcatctccg
      841 aaaagcgggc agacggggag acgacgaccg aggcgaccat aaagctctcc gtaggcggcc
      901 acagggtcat atctaccgca gaaggaaacg gacccgtgaa cgccctggac caggcgctca
      961 ggaaagccat agggccgcac tatccggagc tgaaggagat ccacctctcc aactacaagg
     1021 tccgcatcct cgacgagcac cgcgctacgg cggcgacgac ccgcgtcctc atagactcga
     1081 ccgacggcaa gcggatgtgg ggcgccgtcg gcgtctccga gaacatcatc gaagcctcct
     1141 ggcaggccct ggttgatgga ttggagtacg gcgtgaacgg gaagaaggtg tgatcccggc
     1201 ctccaggtag cttcaggccg ggatccttcg tcagtgagca tcaggacgct cgtcaacccg
     1261 aacgccctgg agacgctgga cggcccgacc ccggatcggg cgccgctgcg attccaccgc
     1321 cgtctgcccg gctacgaggc gacaccgctg gttgacgctc cgggcctcgc cgaagctctt
     1381 ggcgtcggca aagtcttcgt gaaggacgaa tcgagccggc tcgggctgcc cgccttcaag
     1441 gtcctgggcg catcgtgggc ggtatacagg gcgctggagg agcgtctcga ggacgagagt
     1501 tttggggatt gggaggagat cgaagatctc aaggagaagc taagtcccct gcggcagctc
     1561 tcgctcgtgg cggccacaga cgggaaccac gggcgtgccc tggcctgggt ggcgcggcta
     1621 ctgggattcg gggcgaagat cttcgttccc caggacatgg cagcggcgcg ccgcgaggcc
     1681 atcgtccacg agggcgccga ggtgatcgtg gtcgatggca cctacgacga cgccgtcgag
     1741 atctcggctg aagaacgcgg gctcctgatc tcggacatgt cctggcccgg ctacgagcgt
     1801 ataccttcct gggtgataga aggctactcg acgatgctgt gggagataga cgacgagttg
     1861 gagaagagga acgaagcagg accagacctc gtggtcgt
//
LOCUS       DATAIR010000822         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_173813, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000822 DATAIR010000000
VERSION     DATAIR010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173813"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VHM69_19495"
     CDS             <1..1566
                     /locus_tag="VHM69_19495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2742634.1"
                     /translation="LDGSTRSLDEEMLVIADEERGLVVAGVMGAEDAEVGDETEDALI
                     EVATFEGRNIMQTSQKLALRTDASGRFERGLDPNMVEYAMDRVTALLTQVAGGRAAAD
                     TLSHYPEPVEPWRVPLRLERAELLLGMPVEPDEAAERLEALGCEVEREDGRLSATVPT
                     FRRDIRREADLVEEVGRLIGLDRVPETLPAVPQPGGLTEEQHRTRLLRRLLADVGLSE
                     AITYPFGPERWTEDLGLDDGAVRLKNPLSAEGSYLRTSILPGLLDAAARNRAFGARGG
                     ALFEVGHVFFADPPPDDLRDVALSFRMTGERDEQDADRLMGVREENRLGVVLAGTVRP
                     AGWNVPEARAGFFEAKGLVERLVPGASFVPAARPFLHPGRTAVVRVGAEEVGWVGELH
                     PGIAEKFELDGWPVAALELDPAFCQPDPEPRFEAFVNVPAVARDLAVLVEERIPVGDM
                     LRAIRDLPSSVLTEVRVFDVYAGSQVPAGHKSVAFGFIFQGEKTLTDEEVDAEVGHIV
                     ASLEDTFGARVRS"
     gene            complement(1556..>2015)
                     /locus_tag="VHM69_19500"
     CDS             complement(1556..>2015)
                     /locus_tag="VHM69_19500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2742635.1"
                     /translation="IWHLTSWAPSPLSRPAVERLFEDRLHSPTDDSFAIHVRGEVEPI
                     GVISLMNLSGANESADLSVLVGHPDDRHQGYGTEAMERLLRYAFETLGLNRIGLSVFE
                     FNEEAISAYEKLGFVAEGRLRGAIKRSSGFHDAILMSILKTEWEAGAPNS"
BASE COUNT          337 a          612 c          708 g          358 t
ORIGIN      
        1 ctcgacggtt cgacgcggtc gctggacgag gagatgctcg tcatcgccga cgaggagcgg
       61 ggactcgtgg tcgctggcgt gatgggcgcc gaggatgccg aggtggggga cgagacggag
      121 gacgccctga tcgaggtcgc caccttcgag gggcgcaata tcatgcagac ctcccagaag
      181 ctcgccctgc gtaccgacgc ctcgggccgc ttcgagcgcg gcctcgaccc gaacatggtg
      241 gagtacgcga tggacagggt gacggctctg ctcacgcagg ttgcgggtgg aagggccgcg
      301 gccgataccc tcagccatta cccggagccc gttgagccct ggcgcgtacc gctgcgactg
      361 gagcgggcgg agctcttgct cggaatgcct gtggagccgg atgaagccgc cgaacgtctg
      421 gaggccctgg ggtgtgaggt cgagcgggaa gacgggcggt tgtcggcgac cgtgccgacc
      481 ttccgcaggg acattcgccg tgaggctgac ctcgtcgaag aggtcggacg gctcatcgga
      541 ctcgacagag tacccgagac gctccctgcg gtgccacagc cgggtggact tacggaagag
      601 cagcacagga cgcgtttgct caggcgtctg ctcgcggacg tgggactctc ggaggccatc
      661 acctacccgt tcggccccga gcgctggact gaggatctcg gtctggacga tggtgccgtc
      721 cggctgaaga acccattgag cgccgagggc agctatctgc ggacgagcat actgccgggg
      781 ttgctcgacg cagcggccag gaacagagcc ttcggggctc ggggtggggc gctcttcgag
      841 gtgggacacg tcttcttcgc cgatcctccg cccgatgatc tcagggatgt cgccctcagt
      901 ttccgcatga cgggtgagcg cgacgaacag gatgccgacc ggctcatggg cgtgcgggag
      961 gagaacaggc taggggtcgt gctcgccggg acagtgcgcc ccgccggttg gaacgtgcct
     1021 gaagccaggg caggcttctt cgaggccaag ggcctggtcg agcggctggt gcccggcgct
     1081 tcctttgttc cggcggcgag accgtttttg caccctggac gaacggccgt agttcgggtc
     1141 ggcgcggagg aggtcggctg ggtcggagag ctgcatccag ggatcgctga gaagttcgag
     1201 ctcgatgggt ggcctgtcgc cgcattggag ctggatcccg ccttctgcca gcctgatcct
     1261 gagcctcggt tcgaggcttt tgtcaacgtt cccgccgtcg ccagggacct cgcagtcctt
     1321 gttgaggagc ggatacccgt cggcgacatg ttgcgcgcga tacgggacct ccctagttcc
     1381 gtactcaccg aggtacgtgt cttcgacgtc tacgcgggga gtcaggtccc tgccggacac
     1441 aagagcgtgg ccttcggatt tatcttccag ggggagaaga ccctcaccga cgaagaggtg
     1501 gacgccgagg tcgggcacat cgtcgccagc ctcgaagaca cgttcggcgc gcgcgtcagg
     1561 agttaggagc cccggcttcc cactcggtct tgaggatgct catgaggatc gcgtcgtgga
     1621 aaccggagct tcgctttatg gcgccgcgga gacgcccctc cgccacgaag cctagcttct
     1681 cgtaggctga gatcgcctcc tcgttgaact cgaagacgct caggccgatg cggttcagcc
     1741 ccagcgtctc gaaggcgtag cgcaggagcc gttccatagc ttccgtcccg tagccctgat
     1801 gacggtcgtc cggatgaccg acgagcaccg agagatccgc tgactcgttg gccccgctta
     1861 agttcatgag gctgatcaca ccgataggct cgacctcgcc cctgacatga atagcgaagg
     1921 agtcgtcggt gggggagtgc aatctgtcct cgaacaacct ctcaaccgct ggacggctca
     1981 agggcgatgg tgcccagctg gtcaggtgcc agatc
//
LOCUS       DATAIR010000823         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_82756, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000823 DATAIR010000000
VERSION     DATAIR010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82756"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VHM69_19505"
     CDS             complement(<1..396)
                     /locus_tag="VHM69_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742636.1"
                     /translation="MKSENRLVLLLSTAAFVVCTAAAGAGLFSRLDAHMLDLAQREPS
                     QLLDAIGLVTSVAGGVEFVAVAAFALAAGLFVDGQRRLAFRLLTAFVVTGLIEVALKM
                     VVPQAPVPDVVARGSDPSLLDVSTPYPYPS"
     gene            complement(479..1363)
                     /locus_tag="VHM69_19510"
     CDS             complement(479..1363)
                     /locus_tag="VHM69_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX2742637.1"
                     /translation="MKANLEKRRENQKIERESETPELLAKYLAHIGQGNLLTHQEEID
                     LSMRAKAGDRRARQRLIEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDP
                     DRGFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKVSRAMSELALEIEREPT
                     EDEVARRLDWDPDEVRLTMRAMPDATSLDQPVSSEETASQLGDFIEDDRVSDTPDTVM
                     REMETEHLKEAIGRLPERARYVLVRRYGLDDREPATLAELGEELDISRERVRQLQREA
                     ERILKGGEYGRVLRDAVA"
     gene            1603..>2198
                     /locus_tag="VHM69_19515"
     CDS             1603..>2198
                     /locus_tag="VHM69_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742638.1"
                     /translation="MENATRISDRIDEDVAGIVACWQASRGGADEDGVYDRMLSGMED
                     LVGTFVAFLRSTETVETFSQGGAIHVMVGEIIENQHELGRDAVGVIEDFSVLRRCIWR
                     SVEAGVDLSGLDGVEVSGFFAKLMQASDWLTETGLEAFDAIVRREMEQALGQAAATDL
                     VTGLPDRDQLNRLLLPRAIDSHDQFTVAVFDVADFTETV"
BASE COUNT          401 a          714 c          673 g          410 t
ORIGIN      
        1 gctggggtac gggtaaggag tgctcacatc gagcaacgag ggatccgacc cccgcgcgac
       61 cacgtcaggc acgggcgcct gcggcaccac catcttgagt gcgacctcga tcagccctgt
      121 caccacgaac gccgtgagca gccgaaatgc gaggcgacgc tgaccgtcca cgaataatcc
      181 agcggccagc gcgaacgcgg cgacggcgac gaactcgaca cctcccgcca cggacgtaac
      241 caatccgatc gcgtccagga gctgcgaggg ctccctctgt gccaggtcga gcatgtgtgc
      301 gtccaggcgg ctgaacagcc ccgcgccggc cgccgcggtg caaaccacga aggccgccgt
      361 agagagcagc agaaccagcc tgttttcgct cttcacgtcc tcacagacta gcaaagcggg
      421 ggcggcgggg cacgagaaag gccggacccg aaggcccggc caggtcagca aaaacgagtt
      481 aggctacggc gtcacgcagg acgcggccat actcgccacc cttcaagatg cgttccgcct
      541 ccctctgcaa ctgcctcacg cgctcgcgcg agatgtcgag ctcttcgccg agctctgcga
      601 gggtggcggg ctcacggtcg tcgaggccat agcgcctgac gagcacgtag cgcgccctct
      661 cggggagacg cccgatcgcc tccttcagat gttccgtctc catctcccgc ataacggtgt
      721 cgggggtgtc ggagaccctg tcgtcctcga tgaagtcccc gagttgggag gcggtctcct
      781 cgctcgatac cggctgatcc aggctcgtcg cgtcgggcat cgcgcgcata gtgaggcgca
      841 cctcgtcggg gtcccagtca agacgcctgg cgacctcatc ctcggtgggt tcgcgctcga
      901 tctccagggc cagctcgctc atggcccggc tgaccttccg gatcttctct gtcatgtgca
      961 cggggacgcg gatggtacgg cccttgtcag cgacggcccg ctgcacggcc tgacggatcc
     1021 accacgtcgc gtacgtggag aacctgaagc cccggtcggg gtcgaacttc tcgacggcct
     1081 tcatcaggcc gatgttgccc tcctggatca ggtcctcgaa gggaagcccg tagcccctgt
     1141 acttctttgc cacggatacg acgagcctga ggttcttctc gatcagcctc tgccgcgccc
     1201 gcctgtctcc ggccttggcc ctcatgctga ggtcgatctc ctcctggtgc gtcaggagat
     1261 tgccctgccc gatgtgcgcg aggtacttgg caaggagctc gggggtctca gactcccgct
     1321 cgatcttctg gttttctctc cgcttctcca aattagcttt caaaataccc tcctcactta
     1381 cataccaatg gtaccggaaa tcaattttag aaactactat taatttaccg cgaacgttaa
     1441 gtccgtaaac ttctcgaggc ctccatcctc acccgctctg atcgctactc tttgccgcac
     1501 caatacatac gcatgtcggc gcgaaaaagt tgcatgtggg acccgcgggg ctgcgtacag
     1561 ggcgtggtgc gtcgatacgt tgtggcacgc tatactgggg acatggaaaa tgccacgcgc
     1621 atctccgacc gcatagacga ggacgtcgcc ggcattgtgg cctgctggca ggcgagcaga
     1681 ggcggagccg atgaggatgg ggtctacgac cggatgctct ccggcatgga ggacctcgtc
     1741 ggcaccttcg tagctttcct gaggagtacc gagaccgtcg agaccttcag ccagggcgga
     1801 gctatccacg tgatggtcgg tgagatcatt gagaaccagc acgagttggg cagggatgcg
     1861 gtcggggtca tagaggactt cagcgtcctg cggcggtgca tctggcgctc ggtcgaggct
     1921 ggtgtcgacc tctcaggact ggacggcgta gaagtttcgg ggttcttcgc caagcttatg
     1981 caggcctcgg actggcttac ggagaccggc ctcgaggcct tcgatgctat cgtacgccgc
     2041 gagatggagc aggcgctagg gcaggccgcc gcgaccgacc tcgtcacagg acttccggac
     2101 agagaccagc tcaacaggtt gctcctgcca cgcgccatag attcgcacga ccagttcacc
     2161 gtcgcagtct tcgacgtcgc cgacttcacc gagaccgt
//
LOCUS       DATAIR010000824         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_174710, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000824 DATAIR010000000
VERSION     DATAIR010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174710"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VHM69_19520"
     CDS             complement(<1..607)
                     /locus_tag="VHM69_19520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2742639.1"
                     /translation="MRYDVVFLDIDGTLLWVDVDIEGYLEDLAPYSSYGGLTSEKVAG
                     PVWGSLRRHIEGNIRHRTEKDLAGFKLRNAELTAAEIGITAPAEVLTEVAERRISFNP
                     YPESEAVLRRLREMGTRIFAVSNWDIELAKVLDDLGWMGYFEGVIVSAVVGIEKPECE
                     IFEEALRVSGISRDRVVHVGNDPITDVEGALRAGLDTVLVDR"
     gene            complement(609..839)
                     /locus_tag="VHM69_19525"
     CDS             complement(609..839)
                     /locus_tag="VHM69_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742640.1"
                     /translation="MRSYGKILGLVPYDFRLPSPGRARSTFWNVRDERFLVPTFFGIG
                     WTFNLRSAHRHPLQAVLVAAFVLWRIRAARRR"
     gene            complement(1039..1260)
                     /locus_tag="VHM69_19530"
     CDS             complement(1039..1260)
                     /locus_tag="VHM69_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742641.1"
                     /translation="MLRVANVRDEDALESIRDALDLLGVYYEHVGSEPDEDRYPQTAY
                     FDVAYDSKEQVDRALAVLSEERGFDAEVL"
     gene            complement(1279..2382)
                     /locus_tag="VHM69_19535"
     CDS             complement(1279..2382)
                     /locus_tag="VHM69_19535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX2742642.1"
                     /translation="MTDERVGKLARVLVDYSVEAGEGDQVLVFGEVGAEPLIKAVYAR
                     LLQVGAIPITQVSLPGMQELFFEHAKKHHYDEIPGITRAIYEGAHAQIGIRSPSNTRA
                     LANVDPARQQALQRRNKPLGEMMLEKDRWVVTLFPTEALAQEAQMSLSEYEEFAFEAM
                     GLNEEDPVRYWSEKSAEQERLKQRLEVAREIRIVGPGTDLTLSVEGRTFVNSAGRRNM
                     PCGEVFTGPIEDSANGTVYFGVPAAIAGREVSGARLRFEEGKVVEASAEKGEEYLMSL
                     LDADAGARYLGELGIGTNYGIRKASANVLFDEKLGGTVHLAIGRSYAETGGKNDSSVH
                     TDLVCDLREGGELYADGELIQDNGRFLEFDLAG"
     gene            complement(2444..3223)
                     /locus_tag="VHM69_19540"
     CDS             complement(2444..3223)
                     /locus_tag="VHM69_19540"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2742643.1"
                     /translation="MEIPVIFSTGSLYPFGLERVYSWILQAGYDGVEIMMDERWDTHQ
                     EDYLRGLVQMHGIPILALHPPLRRGAWGLKPDQTLVRVARLARKMEVPLVVAHPPPPG
                     RPLERWKAGPLHKAREHGVTVAVENMPRSEAGGIFRVRHRSCYLPEHLAGVGDVTLDT
                     SHAGASRLDLLRTHSTLATQIRHVHLSDSNLVAGRDEHRMPGKGRLPLKPFLAALGTS
                     GYTGAVSLELKPWPLGTPDPETILERMRAALRFTREGLDGH"
     gene            3623..4006
                     /locus_tag="VHM69_19545"
     CDS             3623..4006
                     /locus_tag="VHM69_19545"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2742644.1"
                     /translation="MADPEKNKQTVIAYLNTAFNDKEPTEAVEKYGGSHYIQHNPQAP
                     DGFEAFIQFVTGFVEQFPELSFDVKRAVAEGDLVVTHSLLKTSPEDRGTAAADFFRLE
                     DGKVVEHWDVLQPIPESAANEHPMF"
     gene            4392..4766
                     /locus_tag="VHM69_19550"
     CDS             4392..4766
                     /locus_tag="VHM69_19550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2742645.1"
                     /translation="MLDHVGVPVSDFERSKRFYAEALSPLGYELMMQPSNYAAGFGRL
                     GKPDFWIGQGEPGQAFHIAFAADDRATVDAFYEAAIAAGGRDNGGPGLRPRYHANYYG
                     AFVLDPDGNNIEAVCHKPGEAA"
     gene            4949..5623
                     /locus_tag="VHM69_19555"
     CDS             4949..5623
                     /locus_tag="VHM69_19555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2742646.1"
                     /translation="MPEEFEVNGTTVAGYLALPESGHGPGVLVLHAWWGLTPFFMEVC
                     DRLAAEGFVAFAPDRYGGPTAATIEEAEALQRQREDWVRTEAVLRASVQFLDAHAAVV
                     GEGLGALGFSAGAAWALLLSVREPDLLNAVVAFYGTGPIDYTGARAAYLGHFAEVDEW
                     EPIEGVRGTQEALRAAGCEVTFYTYPGVGHWFFEEDRVGHYDAQAARLAWERTLEFLR
                     ARLGPN"
     gene            5937..>6182
                     /locus_tag="VHM69_19560"
     CDS             5937..>6182
                     /locus_tag="VHM69_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742647.1"
                     /translation="MTTGKLHAGEVDIDIPLVRRLLADQFPQWADLPIRTFQSTGTVN
                     AIYRLGDHLCVRLPRVQRWAQDLEKEWHWLPKLAPSLP"
BASE COUNT         1144 a         1944 c         1915 g         1179 t
ORIGIN      
        1 tgcggtcgac gagcacagta tccaggccgg cccgcaaagc gccctccacg tccgtgatgg
       61 ggtcgtttcc gacgtgtacc accctgtcgc gggagatgcc gctgactcga agcgcctcct
      121 cgaagatctc acattccggc ttctctatcc cgacgaccgc ggatactatc acgccttcga
      181 agtagcccat ccagccgagg tcgtcgagca ccttggcaag ctcgatgtcc cagttggaga
      241 ccgcgaagat tctcgtcccc atctcgcgca gacggcgcaa aaccgcctcc gactcgggat
      301 acgggttgaa ggagatcctg cgttccgcca cctccgtgag gacctccgcg ggcgccgtga
      361 taccgatctc cgccgcggtc aactccgcgt tacggagctt gaagcctgcc aggtccttct
      421 cggtgcggtg ccgaatgttc ccctcgatgt gacgccgaag gctgccccaa acaggaccgg
      481 cgaccttctc ggaagtcagc ccaccgtagc ttgagtacgg cgcgaggtcc tccaaatacc
      541 cttctatatc cacgtccacc caaaggaggg tgccgtcgat atccaggaac acaacatcgt
      601 agcgcatcct agcgtctcct cgcggcgcga atgcgccaca ggacgaaagc cgcaacgagc
      661 acggcctgca gcggatgcct gtgtgcgctc ctcaggttga acgtccagcc gatcccgaaa
      721 aacgtcggga ccaggaagcg ctcgtcgcga acgttccaga atgtgctgcg ggcgcgtccc
      781 gggctcggta atctgaaatc gtatggtacg agcccgagta tttttccgta ggatctcaca
      841 cgtacctccc tacattctac tgcgagagta tagaccgagc ttcggaccgc gaacgtgagc
      901 gttctatcaa tatactcagg tgcggcccac cgccgaggcg cccatgacgg cccagcggcg
      961 gctgatgaaa cgaacgccgc aggtgatgag actcacggtc gtacctcctg gcgtttgggc
     1021 tgaggacctt gccgcctctc aaagcacttc ggcgtcgaag ccgcgttcct ccgagagaac
     1081 cgctaaggct ctgtcgacct gctccttcga gtcgtacgcg acgtcgaagt aggctgtctg
     1141 gggatatcgg tcctcgtccg gctctgagcc gacgtgttcg tagtagacgc cgagcaggtc
     1201 gagggcgtcg cggatactct ccagtgcatc ctcgtccctg acgttcgcaa cgcgcaacat
     1261 cgcctcaccc ggcataattc agcccgcgag gtcgaactcg aggaagcgtc cgttatcctg
     1321 gatcaactcc ccgtcagcgt acagctcgcc gccctcacgc aggtcacaga cgaggtctgt
     1381 gtgcacgctg gagtcgttct tgccgccggt ctccgcgtag gagcggccta ttgcaaggtg
     1441 tacggtcccg ccgagcttct cgtcgaagag tacgttcgcg ctcgccttgc ggatgccgta
     1501 gttggtcccg atcccgagtt ctccgaggta cctcgcccca gcgtcggcgt cgagcaggct
     1561 catcaggtac tcctcaccct tttctgcgct cgcctcaacc accttcccct cctcgaaacg
     1621 aagccgcgcc cccgagactt ctcgtccggc tatcgcggca ggtacgccga agtacaccgt
     1681 cccgttggcc gagtcctcga tgggacctgt aaagacctcg ccgcacggca tgttgcgcct
     1741 tcccgcggag ttcacgaacg tgcgcccttc gacggagagc gtcaggtccg tcccgggccc
     1801 gacaatacgg atctcgcgtg ccacttccaa tctctgtttg aggcgttcct gctcggcgga
     1861 cttctcgctc cagtaccgca cgggatcttc ctcgttcagc cccatcgcct cgaacgcgaa
     1921 ctcctcgtac tcggagaggc tcatctgtgc ctcctgcgcc agcgcctcag tcgggaacag
     1981 cgtcaccacc cagcggtcct tctccagcat catctcgccg aggggcttgt tgcgcctctg
     2041 gagcgcctgt tgcctggcgg ggtcaacgtt cgccagcgcc cgcgtgttgc tgggcgagcg
     2101 gatgccgatc tgggcgtgcg cgccttcgta gatggcgcgc gtgatgcccg gtatctcatc
     2161 gtagtgatgc ttcttagcgt gctcgaagaa gagctcctgc atccccggca ggctcacctg
     2221 agttataggt atggccccga cctgcaagag ccgcgcgtag accgccttga tgaggggttc
     2281 cgcgccaacc tctccgaaga cgaggacctg atccccctcc cccgcctcga ccgagtagtc
     2341 cacaaggacc cgcgcaagct ttccgacccg ctcgtctgtc atgcatagct ccttcctgaa
     2401 gaaacacccg gagagcttat catccgcacg cgggggaagg acgctagtgc ccgtcgagtc
     2461 cttctcgcgt gaagcgcagc gccgcgcgca tcctctcgag gatagtttcg gggtcagggg
     2521 tgccgagcgg ccagggcttg agctccaggc tcacggcgcc ggtatacccg ctcgtcccga
     2581 gcgcggccag gaagggcttg aggggtagac gtcctttacc cggcatgcga tgttcatcac
     2641 gtcccgccac caggttggag tcggagagat ggacgtggcg gatttgggtg gcgagggtcg
     2701 aatgtgtccg aaggagatcc agcctgctcg ccccggcgtg gctcgtgtcg agcgtcacgt
     2761 caccaacgcc ggcgaggtgc tctggcaggt aacagcttct atgccgaacc cggaagatgc
     2821 ctccggcctc gctgcgcggc atgttctcga cggccacggt gacgccatgc tcgcgtgcct
     2881 tatggagcgg tcccgccttc cagcgttcaa ggggcctgcc cggcggaggc gggtgtgcaa
     2941 ccaccagagg cacctccatc ttccgggcga gccttgcgac ccgaaccagc gtctgatctg
     3001 gtttcagtcc ccaggcgccg cgtcggagcg gaggatgtag ggccaggatc gggatgccat
     3061 gcatctgcac gagccccctc agatagtcct cctggtgggt atcccagcgc tcgtccatca
     3121 tgatctcgac cccgtcatag ccagcctgta agatccagga gtacaccctt tcgaggccga
     3181 atggatagag cgagccggtg ctgaagatga cggggatctc catcaactat cgccgcgaag
     3241 gtcactcgtg ggaatacgtg ccccgagtgg tgcggatatc agcgtcgcca tgcgctattc
     3301 gagatcgagg tcctgtaagt aaacgacttg cgcttcttcc ccgccggggt atcgaaagac
     3361 aacttgaagc gccaagggtg ggtcttcggg agccgctcga gcgtgccttc gcctggtgcg
     3421 cggcgtcgtg cgctgttcat gcaacacagg gtagcgcgag agcggggcgg gcgctagttc
     3481 acccaccttg ccgaggattc tcagaaactc gctgccgctg gtaacgctct agcaaccaga
     3541 agatatacca tatgggggat gcgtaatgct cgcccgcaga gcatcatgct aacggcggca
     3601 aggagtacac aaaggaggag ctatggccga ccccgagaag aacaagcaga cggtgatcgc
     3661 ctacctcaac acggcgttca atgacaagga gcccaccgag gcggtagaga agtacggcgg
     3721 atcgcactac atccagcaca atccccaagc acccgacggg ttcgaggcgt tcattcagtt
     3781 cgtcacgggt ttcgtcgagc agttccctga gctgagcttc gatgtcaagc gcgccgtcgc
     3841 cgagggtgac ctggttgtga cgcacagcct tctgaagacc tcgcctgaag accgcggcac
     3901 cgcagcggcg gacttcttcc gcctggagga cggcaaggtc gtcgagcact gggacgtcct
     3961 gcagcccata ccggagagtg cagcgaacga gcaccccatg ttctgatagc ttgcggcgcg
     4021 ggtgtcaggt gggccttcgg tcgtcagagg aaggcgctgg cgggatgcat tgaaggacga
     4081 accctgaccc taaccctccc gacaggagac tcgacttcat tgcttgcttc attcacctga
     4141 cagcgcacta cataagtatt gatccacctc gccccagagg tgcaccttag cgaccaccgt
     4201 ccgtccggga agaccttcct gatgcctgaa acctgatgcc cggcttgtgg gaggatcatt
     4261 ccttccgcaa gccgctgtga gtgcgtagat attggagttc ttggaagttc gccgttgtgg
     4321 aggagcgcgt aacgaggata ctcagcctga cggatgtcgg ttcggtcctg ggaccgtagg
     4381 aggtacgagg agtgctggat cacgtcggtg ttcccgtgtc ggatttcgag cggagcaagc
     4441 gcttttacgc ggaggccctc tccccgctgg gctacgagct catgatgcaa ccgagcaact
     4501 atgcggcggg gttcgggcgg ttgggcaagc cggatttctg gatcggccag ggcgagccgg
     4561 gccaggcctt ccacatagct ttcgccgccg acgaccgcgc caccgttgac gccttctatg
     4621 aggcggccat cgcggcgggc gggcgcgaca acggcggtcc cgggctgcga cccaggtacc
     4681 atgcgaacta ctatggggcc ttcgtcctgg atcctgacgg caacaacatc gaagcggtct
     4741 gccataagcc aggagaagcg gcctgagaca tagagctcct attcacccaa cagcgtagaa
     4801 ggtgaacgtc taggaacacg ccttttcccg gaaggtcggc tgataggccg ccgggtgatg
     4861 gcgtattcgg ttcgataact tcctcttggc aaacaaacaa gttcgaggac actctaggta
     4921 agtagtcatc gagtagggag tagcggggat gccggaggag ttcgaggtca acggcacgac
     4981 ggtggcgggg tatctggcgc tacccgaaag tgggcatggt cccggggttc tcgtcctgca
     5041 tgcctggtgg gggcttactc ccttcttcat ggaagtgtgc gatcggctcg ccgccgaggg
     5101 cttcgtcgct ttcgccccgg accggtacgg cggtcccact gcagcgacga tcgaagaggc
     5161 cgaggcccta cagcgacagc gagaggactg ggtgcgcaca gaggcggttc tgagggcttc
     5221 ggtgcagttc ctggatgccc atgcagccgt ggtcggtgag gggcttggcg ccttgggctt
     5281 ctcggccggg gccgcctggg cgctgttgct ctcggtgcgc gagcccgatc ttctgaacgc
     5341 cgtcgtcgcc ttctatggca cagggccgat cgactacacc ggggcgcggg cggcttacct
     5401 gggtcacttc gccgaagtcg acgagtggga gccaatagaa ggagtgcgag ggacgcaaga
     5461 ggcactgcgt gccgccgggt gcgaggtgac gttctacacc taccctggcg tcggacactg
     5521 gttcttcgag gaggaccgtg tcggccacta cgacgctcag gcggctcgcc tggcctggga
     5581 acgaacttta gagttcctgc gagcacgcct tggtccgaac taatactcgt tagtatgtgg
     5641 gctttctaac tgccgcactc gccgaagtac ctggaaaagc tgtctgccag gaagttcgca
     5701 ttggcccggt gtgcttgagg gtatgcgggc ccgtcagcac cacgttccat aatgtctccg
     5761 gtacgaataa tggatcccgc caaaccaggc cagatggaca actgcagcga atcttatcgg
     5821 gtagtcggtt aagcttgggc gtgtctacag ggtgcggccg cggacggaca acaacccgat
     5881 ctaccgcgag gagtgcctgc tatgcgctgc gcatctgact cgaaaaggag acctccatga
     5941 caacgggcaa gttacacgcc ggcgaggtcg acatcgacat acctctcgta cgaaggctgc
     6001 tcgccgatca gttcccgcag tgggcggact tgccgatcag aacgtttcag tcgacgggga
     6061 cggtgaacgc catctaccgg cttggcgacc acctctgcgt ccgactgccg cgcgtgcaga
     6121 ggtgggcaca ggacctggag aaagagtggc actggcttcc gaagctcgct ccaagtctgc
     6181 ca
//
LOCUS       DATAIR010000825         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_175495, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000825 DATAIR010000000
VERSION     DATAIR010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175495"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1658
                     /locus_tag="VHM69_19565"
     CDS             <1..>1658
                     /locus_tag="VHM69_19565"
                     /inference="COORDINATES: protein motif:HMM:NF036809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX2742648.1"
                     /translation="PSLWWTHDLGEPVLHDLRVELRQDYEMIDQYRSKVGIRTLELDQ
                     SPDPDEPGTRFFRFVLNGVPIFARGADWIPADSFVGSIPDERYETLISAARDANMNML
                     RVWGGGIYEHDVFYDLCDSMGLLVWQDFMFACAVYPEEPPSFVAEVEAEARYQVRRLR
                     SRPSLALWCGNNENQWSHDRTFWDRDDTTVPGALYYHDVLPRVVAELDDHTPYWPGSP
                     FGGDDHNDRRQGNVHNWEVWHGNFPRHFGEESRQDPTPENVSYLRYAEDMGRFISEFG
                     MHAAPVRETLRRAIPEDQLYHHSPSMDHHNKDDPKNKGDNLMVNVTGLPEDLDEYVDF
                     SMISQAEGLKFALEHFRRRKPHCSGTLFWQLNDCWPVLSWSVVDYNGFGKAGYYYARR
                     AYSPILASFKTLPDGSIELWLTNDTLSNVEDDVTVRLATFEGETIWEESFVAHVPSNA
                     SRSVARWSADHTPGAPDRYLSVRSARDHFSANRHFFAAIKDLRRTPVEPDMDITPIGE
                     HELEVRLTTSAYAYFVHLEVPDAATRFSDNYLDLEPGEERTISV"
BASE COUNT          339 a          583 c          451 g          285 t
ORIGIN      
        1 atccaagtct ctggtggacc cacgaccttg gcgagcccgt cctgcacgac ctgagggtgg
       61 agctacgtca ggattacgaa atgatcgacc agtacagatc gaaggtcggc ataaggacgc
      121 tggagttgga tcaatcacca gaccccgacg agccgggtac gcgcttcttc cggttcgtat
      181 tgaacggcgt cccgatcttc gcccgcggcg cggactggat ccccgccgac tccttcgtcg
      241 gctcgatccc tgacgaacgc tacgagaccc tcatctcagc ggcccgagac gccaacatga
      301 acatgcttcg cgtctggggc ggcggcatct acgagcacga cgtcttctac gacctctgcg
      361 actccatggg cctcctggtc tggcaggact tcatgttcgc ctgcgccgtc taccctgaag
      421 agccaccctc cttcgtcgcc gaggtcgaag cggaggcacg ctaccaggtg cgccggctgc
      481 gcagccgccc gtccctcgcg ctctggtgcg gcaacaacga gaaccagtgg agccacgacc
      541 gcacgttctg ggatagggac gacacgaccg ttcccggtgc tctctactac cacgacgtcc
      601 tgccccgcgt cgtcgccgag ctcgatgacc ataccccata ctggcctgga agcccatttg
      661 gcggcgacga ccacaacgac cgcaggcagg gtaacgtaca caactgggag gtctggcacg
      721 gcaacttccc gcgccatttc ggtgaggagt cccgccagga cccgacgccg gagaacgtct
      781 cctacctccg ctacgccgag gacatggggc gcttcataag cgagttcggc atgcacgccg
      841 ccccggtccg cgagaccctc cgccgcgcga taccggagga ccagctctat catcacagcc
      901 cctcgatgga ccaccacaac aaggacgacc ccaagaacaa aggcgacaac ctcatggtca
      961 acgtgacggg cttgccggag gaccttgacg agtatgtgga cttcagcatg atctcacagg
     1021 ccgaaggcct gaagttcgcc ctcgaacact ttaggcgccg caagccccac tgctcgggta
     1081 cccttttctg gcagctcaac gactgctggc ccgtactttc gtggagcgtc gtggattaca
     1141 acggtttcgg caaggccggc tactactacg ccaggcgcgc ctactccccg atcctcgcct
     1201 ccttcaaaac actaccagac ggctccatag aactctggct aaccaacgac accctctcga
     1261 acgttgaaga cgacgtcacc gtgcgcctgg cgaccttcga aggagagacc atctgggaag
     1321 agagcttcgt ggcgcacgtt ccttccaacg ccagtcgttc cgtggcgcgc tggagtgccg
     1381 accatacccc tggcgccccc gaccgctacc tctcggtacg ttcggcaaga gatcacttct
     1441 ccgccaaccg ccacttcttc gcagccatca aagacctgcg gcgaacaccg gtcgagccgg
     1501 acatggacat caccccgatc ggggaacacg agctagaagt tcgcctcact acctcagcgt
     1561 acgcctactt cgtccacctc gaagtcccag acgcggcgac ccgcttctcg gacaactatc
     1621 tcgatctgga accgggagaa gagcgcacca tctcagta
//
LOCUS       DATAIR010000826         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_176333, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000826 DATAIR010000000
VERSION     DATAIR010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176333"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VHM69_19570"
     CDS             <1..1418
                     /locus_tag="VHM69_19570"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2742649.1"
                     /translation="SVFALTSVIGPTMGGWITDNLSWRWVFYINLPLGVFSLFALPVV
                     LHQGARRYGVKIDYLGAATITASVVSLLLALSWVGEGYDWDATRVVIGFVVAAVLLAA
                     FIPVELRATEPIIPLSLFKSRVFGSAALLMFMVGIGMFGIILYTPLFVQGVLGKSATG
                     SGTVLTPLVLSMTAMGVICGQIIARVKRVKPFMIIGAIVMTAGIYLLTTLDVDSTQTT
                     VALYLMVTGFGLGPLMPSATLAVQSTVEKRLLGVATSATQFIRSLGSTVGTAVIGSLV
                     TSGYAVYLKDNAPPQAPGRLINALKDPNALVSDQARDALDRVASAFPGGEQIVNQILD
                     VAREGLSKSIHDGFVFTLVAVMCAFVAALLMKNIHLGEERMEAAPVATNIRQDRLVTG
                     IVLEHLARRIESANGDSRELISAASRLVPDAEGSERERARIAVDHVLRPLALQALSAS
                     VERNGRRDPVEERIQSRPRSS"
     gene            1472..1562
                     /locus_tag="VHM69_19575"
     tRNA            1472..1562
                     /locus_tag="VHM69_19575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1507..1509,aa:Ser,seq:gga)
     gene            complement(1778..>2414)
                     /locus_tag="VHM69_19580"
     CDS             complement(1778..>2414)
                     /locus_tag="VHM69_19580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2742650.1"
                     /translation="HLALVAADVSTQTKVEFPLMAPLYWDDSDSVNPALYVRASMSIP
                     GFFYPLRVPNVPQGAEARQRWTDLASFEEDPPETATLVDGGIMSNFPIDLFHVPDKEP
                     AAPTFGAKLGPDERKFAEIDSPQQLGAAVFSAASHTLDYDFIKRNPDYERLVAYIDTG
                     NHGWLNFDMSDEDKIDLFVRGARKAAEFLTIFDWEKYKEIRRLKKELYTQV"
BASE COUNT          430 a          744 c          717 g          523 t
ORIGIN      
        1 tcagcgtctt cgcgctaacc agcgtcatcg gtccgacgat ggggggttgg atcacggaca
       61 acctctcctg gcggtgggtc ttctatatca atttgcctct cggtgtcttt tcgctgttcg
      121 cattgcccgt cgtgctccat cagggcgcca gacgttacgg ggtgaagatc gactacctcg
      181 gcgcagcgac cataacggcc tccgtcgtct cgctgctgct ggcgctctcg tgggtcggtg
      241 agggctacga ctgggatgcc acacgggtag tcatcgggtt cgttgtcgcc gccgtattgc
      301 tcgccgcctt catccccgtc gagctcaggg cgaccgagcc catcataccg ctctcgttgt
      361 tcaagagcag ggtcttcggt tccgccgcgc tgctcatgtt catggtcggc atcgggatgt
      421 tcgggatcat cctgtatacc cctctcttcg tgcagggcgt gctcggcaag agcgcgacgg
      481 gctcgggaac ggtgcttacg cctctcgttc tcagcatgac ggccatgggg gtgatctgcg
      541 gccagatcat tgcccgcgta aagcgcgtca aaccgttcat gatcatcggc gctatagtga
      601 tgaccgctgg tatctacctg ttgactacgc tggacgtgga ctccacccag acgaccgtcg
      661 ccctctatct catggtcaca ggcttcggac tcgggccgct catgccgtcg gctaccctcg
      721 ctgtccagag cactgtcgag aagagactac tcggcgtcgc gacctccgcc acgcagttca
      781 tacggtccct gggatcgacg gtcgggacgg cggttatcgg ctcgctcgtg acctcgggtt
      841 acgctgtgta cctgaaggac aacgcaccgc cgcaggcccc aggccgcctc ataaacgctc
      901 tgaaagaccc gaacgcgctg gttagcgacc aggcgcgaga cgccctcgac cgcgtcgcct
      961 ccgccttccc cggcggggag cagatcgtca accagatcct cgatgtcgcg cgtgagggcc
     1021 tctcgaaatc catccacgac ggcttcgtgt tcaccctcgt cgccgtgatg tgcgcattcg
     1081 tcgccgccct cctgatgaaa aacatccatc tcggcgagga gagaatggag gccgctccgg
     1141 ttgcgacgaa catcaggcag gacaggcttg tgaccggaat cgtcctcgag catctcgcca
     1201 gacgcatcga gagcgccaac ggcgactctc gggaactgat ctccgccgcc tccaggctgg
     1261 tccccgacgc cgagggctca gagagggagc gcgcacggat tgccgtggat cacgtcctgc
     1321 gccctcttgc gctgcaagcc ttgagcgcga gcgtcgagcg caatggcagg agagacccgg
     1381 tcgaggagcg catccagtcc cggccgcgtt cctcctagcg tcgcctgtag cagcggattg
     1441 gctttttggt aaactcacgc ccgtcgcttc cggagaggtg tccgagttgg ccgaaggagc
     1501 gcgattggaa atcgcgtagg cggtgttaag ccgtctcgtg ggttcgaatc ccaccctctc
     1561 cgctttctct cttatttgca ggtgaatcgt aggattggta ggagacaagt agatgaggct
     1621 cgagggtccc gaggcctcat ctactgtaac agtactgcaa cccgctaaga acgtacctca
     1681 tcaatggtgg tggcagtctg atctataata ctcggcaaca cgtggaagta ggtgtcgagc
     1741 tagacccatt ccgctgcgct ccccaacgca tctgctccta aacctgtgta tagagctcct
     1801 tcttgagtcg acgtatttcc ttgtacttct cccagtcaaa gatggtaagg aactcggcgg
     1861 ctttgcgggc gccacggaca aagaggtcga tcttgtcctc gtcgctcatg tcgaagttga
     1921 gccaaccgtg attccccgta tcgatgtagg caacaagcct ctcgtagtcg gggttgcgct
     1981 ttatgaagtc gtagtcgagg gtatggcttg ccgcgctgaa gaccgcggcc cctagctgct
     2041 gcggactatc tatctcggca aacttcctct catcgggacc gagcttcgcc ccgaaggtgg
     2101 gcgctgcagg ctccttgtcg ggtacgtgaa ataggtctat tgggaagttg gacatgatcc
     2161 cgccatccac taaggttgcc gtctccggcg gatcttcttc aaagctcgcg agatcggtcc
     2221 acctttggcg tgcttccgct ccctgcggca catttggaac ccgcagcggg tagaagaagc
     2281 ccgggatgga catcgacgcc cttacgtata gtgccgggtt tacgctgtcc gagtcgtccc
     2341 agtagagtgg cgccataagg ggaaattcga ccttcgtctg cgtcgagacg tccgctgcaa
     2401 ccagcgctag gtga
//
LOCUS       DATAIR010000827         7741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_176811, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000827 DATAIR010000000
VERSION     DATAIR010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176811"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..354
                     /locus_tag="VHM69_19585"
     CDS             160..354
                     /locus_tag="VHM69_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742651.1"
                     /translation="MDLTTAPNAKAAAVPQITPITMPKATSLLHHKTPTLTSTATKKN
                     TTVDNITVLASLARILHPIV"
     gene            complement(441..926)
                     /locus_tag="VHM69_19590"
     CDS             complement(441..926)
                     /locus_tag="VHM69_19590"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2742652.1"
                     /translation="MATLSVLKFDDPSGAYRVLIALQGLQEREMITLEDATVVSWPEG
                     NNKPKASQQHGNFDVGAISGAFWGFLIGVIFFVPSLSVGAGALSSPLADVGIDDDFIR
                     QVREKVTEGTSALFALTSGVTALDEVGDELKHYDFEIISTSLPEEQERQLREAFAHEE
                     E"
     gene            complement(1169..1381)
                     /locus_tag="VHM69_19595"
     CDS             complement(1169..1381)
                     /locus_tag="VHM69_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742653.1"
                     /translation="MNAGPEESSSRAHEESEARIIDVADLAHSETAYEFEGHHHGDTN
                     VSFIVVSDTNATRGACPCSAHLGHSG"
     gene            complement(1472..2668)
                     /locus_tag="VHM69_19600"
     CDS             complement(1472..2668)
                     /locus_tag="VHM69_19600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2742654.1"
                     /translation="MSAGGLSEARLGRMHDVMAGHVERGEVPGLVTLVSRRDEVYIDA
                     IGMKAVGGNDPIQRDTIFRIASMTKPITAAAAMVLVEECKLRLDDPVDSLLPELADRK
                     VLKAIDSALDDTVPANRQITVRDLLTLRLGIGAVMAPPGRYPIQEAMDEARLAPGPDS
                     PSLAPDEWMKRLGGLPLIHHPGEKWMYETGSDVLGVLISRSTGQPLETFFRERIFDPL
                     GMKDTGFHVPAENLHRLASCYVANSETGTLDLYDDVDDSKWSRPPAFESGGGGLVSTV
                     DDYLAFCRMMLNKGKHGGERILSRPSVVLMTTDQLTPEQRAEADILLGDNSSWGFGMS
                     VVTRRDDLASTPGRFGWDGGYGTSGYSDPKEDMVGIMMTQRLAGPHSPLIDRDFWTSA
                     YQAIDD"
     gene            complement(2665..3492)
                     /locus_tag="VHM69_19605"
     CDS             complement(2665..3492)
                     /locus_tag="VHM69_19605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2742655.1"
                     /translation="MNDTPKGDYAEVNGVNMYYEIHGTGKPLVVLHGAYMTIDAMGEI
                     VPALAETRRVIAVELQGHGRTADIDRPITYEHMADDVAALLRHLEIENADIFGYSMGG
                     AAAIQVTIRHPDVVRKLVVASASYTSDGAHPEMLEMIPSITPEAFAGSPLEEDYLRTA
                     PNPEDFPTLVAKLKQLDMEPFAWPQEDIRGISAPTLLIIGDSDVVRLEHAVELFRLLG
                     GGVMGDLAGLPRSQLAVLPGTSHFVPPGSGMLDRADWLLSMIPPFLDAPMPETGNRR"
     gene            complement(3533..4246)
                     /locus_tag="VHM69_19610"
     CDS             complement(3533..4246)
                     /locus_tag="VHM69_19610"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX2742656.1"
                     /translation="MSLPKVVSRDEWLAARKELLAKEKEFTRQRDTLNAERRMLPMVR
                     IDKDYVFEGPEAKAGLLDLFEGRRQLIVVHFMFDPGWDAGCSSCSASADEISDGLLEH
                     LHTRDTTLVHVSRAPLAKIEAYKASKGWTFPWYSSYGSDFNYDFHVTLDESVVPAEYN
                     YRTDVEGEQPSEAPGRSCFLREGDNVFHTYSVYARGLETVGGSYYFLDETALGRQEEW
                     EEPKGRAADARGAIPDFSS"
     gene            complement(4290..4682)
                     /locus_tag="VHM69_19615"
     CDS             complement(4290..4682)
                     /locus_tag="VHM69_19615"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX2742657.1"
                     /translation="MEINYLAIGVAVVVAFVASTAWYTVFGAERDRLLGTPGAASERP
                     PVWMMLVELVRSFVVAYVLAVLVGLAGVAGFFGAVGIGLMMWVGFPVVLLVGSVIWDR
                     VPFKLAAIHAGDWLVKLLLMAVIVGLWR"
     gene            complement(4712..6097)
                     /locus_tag="VHM69_19620"
     CDS             complement(4712..6097)
                     /locus_tag="VHM69_19620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2742658.1"
                     /translation="MGESISKKGQVLFVHGGGEGAHEEDKKLAENLQSVLGAAYDVLS
                     PKMPDEHSPEYAAWRDRISQELTALDGEVFLVGHSLGGSVLLKYLSEEKVEKPIAGIF
                     LISAPYWGAEDWQVSEYELKEDFASELRGELPAFFYHSRDDEVVPFVHLAMYKERFPQ
                     ASFREFDGRGHQLNGDLSEVALDIERRRNAMTENKGNYAEVNGLEMYYEVHGSGDPLI
                     LLHGGVGAIEMFGEVLPMLAEGRQIIGVDLQAHGRTADIEGRPLSYEHMADDVAALIE
                     HLQIERADVMGYSLGGGVALQTAIRHPGVVRKLVVVSTTFKRDGWYPEVLAGMEQMGP
                     EAAEPMKATPMYQLYSGIAPRPDDWPLLLTKLGDLLRQAYDWSEDVAAMEVPTMIVVG
                     DADSVRTAHAVELFELLGGGRADGGWDRSGMSDARLAILPATTHYEIFFSPTLASTVT
                     PFLDENVTEAD"
     gene            complement(6104..6571)
                     /locus_tag="VHM69_19625"
     CDS             complement(6104..6571)
                     /locus_tag="VHM69_19625"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX2742659.1"
                     /translation="MAEQDAQRQAPEPNSALGSLEVMVGTWDLKGRESGPDGEIHGRV
                     TFEWMEGGFYLVQHVDLDHIGNRIKGVEYIGYDESNEVLKSYFFGNTGPGPFGGVALE
                     YVWEVGDDTLTIWGGFVGSPAAFKGMFGDNGDTITGRWEWPGGGYEATMTRVE"
     gene            complement(6797..7159)
                     /locus_tag="VHM69_19630"
     CDS             complement(6797..7159)
                     /locus_tag="VHM69_19630"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX2742660.1"
                     /translation="MNDELLAMVDREVLGWPGVSKERHEGGSGRGGFRVPPATIYKLG
                     RRSLGHIHDTGVADIAFPREIHDVLISEGKAKQHAAGFAGVVSYRVREPEDVPGAVDL
                     FRLSYDRAKSAAERRQSA"
BASE COUNT         1472 a         2521 c         2177 g         1571 t
ORIGIN      
        1 ggagaagaac tagacgcacg actcgtccgg gagcgtaccc tacgaagtcg ggttaataga
       61 gaagaggcct cgtgtttcga aaggtcaaac cttgctcagc catcgtgtcc atcccgagat
      121 cgctcgcgtt cggcgccgaa gcgctcctcc gatcggccca tggacctgac aacggcgccg
      181 aacgcaaaag ccgccgccgt accgcagatc actcctatca cgatgcccaa ggctacatca
      241 ctgctccacc acaaaacgcc cacactcacc agcaccgcca ctaagaaaaa caccaccgtg
      301 gacaacatca cggtgcttgc gagccttgcg cgcatccttc accccatcgt ctgactacgc
      361 ctctgtgata cgcccgatcc acttcgcctt agagcggtca ctcaccgatg atcgctctaa
      421 ggcgatgtcg ctcctctagc ctactcctcc tcgtgtgcga aggcctcgcg caactgccgc
      481 tcctgctctt cgggcaaact cgtcgagatg atctcgaagt cgtagtgctt gagctcgtct
      541 cccacctcat ctagcgccgt aaccccgctg gtcaaggcaa acaatgcgga ggtgccctcc
      601 gtcactttct cacgcacctg cctgatgaag tcgtcatcga tgccgacgtc cgccagtgga
      661 ctgctgaggg cgccagcccc gacactgagg gagggcacga agaagatcac cccgatcagg
      721 aagccccaga aggcgccgct tatcgccccc acgtcaaagt tgccgtgttg ctggctggct
      781 ttgggcttat tgttgccctc aggccagctg actacggtcg cgtcctcaag ggtgatcatc
      841 tctcgctcct gcagaccctg aagggcgatg agtaccctgt aagccccact gggatcatcg
      901 aacttcagca ccgatagcgt cgccaagtgc gtttctcctt tctccgttcc gactcgctta
      961 tctacggcgg gccattgttc tccatcgcac acatgatgcc ccgaggacga gtttcgcgca
     1021 tcccctccct atacgcgagg cttccttcgg tcatctcctc gaacttctca gaacgccgta
     1081 catgcgaact cctccgcaga atccatcctg cacacagcgg gaacaagcgt ctaggcgtta
     1141 gaccagcgcc atctcaacct ccggctcctc acccactgtg gccgagatgc gcagagcaag
     1201 ggcaagcccc tcgcgttgcg tttgtatcac tgaccacgat gaacgagacg ttggtatcac
     1261 cgtggtggtg tccctcgaac tcgtaagcgg tctcgctgtg ggccaggtcg gccacgtcga
     1321 taatgcgcgc ctcactttcc tcatgagccc gactgctgct ttcttccggt cctgcgttca
     1381 cgtcgccctc cttcatcggc ggtggtgctc acgacactaa acctaaccag gctgccgcag
     1441 aaacgcacca tcgtctgtgc cataaacagc ctcagtcgtc gatcgcctgg tatgccgagg
     1501 tccagaaatc gcggtcgata agcggggaat gggggcctgc caggcgttgg gtcatcatga
     1561 tgccgaccat gtcttccttg gggtcagaat acccggacgt gccgtaacct ccatcccagc
     1621 cgaagcgtcc aggggtcgat gccagatcgt cgcgcctggt gaccacggac ataccgaatc
     1681 cccaactact gttgtccccg aggaggatgt ccgcttccgc cctctgctcc ggcgtcagtt
     1741 ggtcggtggt catgagcacg accgagggtc ttgacaggat gcgctcgccg ccgtgtttgc
     1801 ctttgttcag catcatccgg cagaaggcca ggtaatcgtc gacggtcgag accagccccc
     1861 cgccgccgga ctcgaaggca ggagggcgac tccacttgct gtcgtcgaca tcgtcatata
     1921 gatcgagtgt cccggtttcg gaattggcca cgtagcagct cgccagacgg tggagatttt
     1981 cggctggcac gtgaaatccg gtgtccttca tcccgagcgg atcgaagatg cgctcgcgga
     2041 agaacgtctc cagcggctgg cctgtagagc gtgagatcag gactccaagc acgtcggagc
     2101 ccgtctcgta catccacttc tcgcccggat gatggatcag cggcagaccg ccgaggcgct
     2161 tcatccactc atcgggcgcg agcgaaggcg agtccgggcc gggggctagt ctcgcctcgt
     2221 ccatcgcctc ctggatcggg taccgtcctg ggggagccat caccgccccg attcccagcc
     2281 gcaaggtcag caggtcgcgc acggtgatct ggcggttcgc aggcacggtg tcgtcgagcg
     2341 cgctgtcgat cgccttgagt actttgcggt ccgccagttc gggcagcagg gagtcaacag
     2401 ggtcgtccag ccgcagcttg cattcttcga ccagaaccat cgccgccgct gcggttatcg
     2461 gcttggtcat cgaggcgatg cggaagatgg tgtcgcgttg tatcgggtcg ttgccgccga
     2521 ctgctttcat gcctatggca tcgatataca cctcgtcccg ccggctgacc agcgtaacga
     2581 gaccgggcac ttccccgcgc tcgacgtggc ccgccatcac gtcgtgcatg cggcctagcc
     2641 gcgcctcgga caatcctccc gcactcatcg tctgttccct gtctccggca tgggcgcgtc
     2701 gaggaacggc ggaatcatcg acaggagcca atcggcgcgg tccagcatcc cactgccggg
     2761 ggggacgaag tgcgacgtgc cagggagcac cgcgagttgg gacctcggga ggccggccag
     2821 gtctcccatc acgccacctc cgagtagccg gaacagctcg acggcgtgct cgaggcggac
     2881 gacgtcagaa tccccgatta tgagcagggt aggcgccgag atcccccgaa tgtcctcctg
     2941 cggccaggcg aagggctcca tgtcgagttg cttcagcttg gcaaccagcg tggggaaatc
     3001 ctccgggttc ggcgcggtcc ggagatagtc ttcttcgagc ggggacccgg cgaatgcttc
     3061 cggcgtgatg gacggtatca tctcaagcat ctcggggtgc gctccgtcac tcgtgtaaga
     3121 ggctgaggcg accaccagct tgcgcacgac atcagggtgc ctgatcgtga cctgaatggc
     3181 ggcggcgcct cccatgctgt agccgaagat atcggcattt tcgatctcca ggtgccgcaa
     3241 cagggcggcc acgtcgtcag ccatgtgctc gtatgtgatc ggacggtcga tgtcggccgt
     3301 gcgaccgtgc ccctgcagtt cgacggcgat gacccggcga gtctcggcga gggcgggcac
     3361 gatctctccc atggcgtcga tcgtcatgta cgcgccgtgg agcacgacca gcggtttccc
     3421 tgtgccgtgg atctcgtagt acatgttgac gccgttcacc tctgcgtagt cgcctttggg
     3481 ggtatcgttc atttcgtctc ctttcgggct cattgccacg gatgctcgcg gttcacgacg
     3541 agaagtctgg gatcgcgcct cgcgcgtcgg cggcgcggcc cttgggctct tcccattcct
     3601 cctgccttcc gagcgcggtc tcgtcgagga agtagtacga gccaccgacc gtctcgagcc
     3661 cgcgggcata gacggagtac gtgtggaaga cgttgtcgcc ctctcgcagg aagcagcttc
     3721 gacccggcgc ctcggagggc tgctcgccct cgacatccgt cctgtagttg tactcggcgg
     3781 ggacgacgga ctcgtccagc gtgacgtgga agtcgtagtt gaagtcgctt ccgtacgagg
     3841 agtaccaggg gaatgtccat cccttgctgg ctttgtaagc ttcgatcttg gccagcggcg
     3901 cacgcgagac gtgcacgagc gtggtgtcac gggtgtgcag atgctccagt agaccgtcgg
     3961 agatctcatc ggcactggcc gaacaactcg aacagcctgc gtcccaaccc gggtcgaaca
     4021 tgaagtgtac gacgatgagc tggcggcgtc cctcgaataa gtcgagcagg cccgccttcg
     4081 cttccggacc ctcgaagacg tagtccttgt cgatcctgac catgggtagc atgcgacgct
     4141 cggcgttcag cgtgtccctc tgtctggtga attccttctc tttggcgagc agctctttgc
     4201 gggcggcgag ccactcgtct cgcgaaacga cttttggaag actcatcgtg ctctccttct
     4261 tgttgtgatc gacattctta ccggtcggac tagcgccata agccgactat gacagccatg
     4321 agaagtaact tcacgagcca gtcgcccgca tggatggcgg cgagcttgaa cggcactctg
     4381 tcccatataa ctgagccaac gagaagcacg acgggaaaac cgacccacat cattaacccg
     4441 atacctaccg ccccgaaaaa cccggcgacg ccggcgaggc cgaccaggac ggcgagcaca
     4501 tacgcgacga cgaagctacg aacgagttcc acgagcatca tccagaccgg cggtctctcc
     4561 gatgcggctc ctggcgttcc caacaacctg tcccgctccg cgccgaacac ggtgtaccat
     4621 gccgtgctcg cgacgaaagc cactacgact gcaactccga tggccagata gtttatttcc
     4681 atgtccagtc ctaatcctgt agtcctcgcg ctcaatcggc ctccgttacg ttctcgtcga
     4741 ggaacggcgt gaccgtcgaa gccagcgtgg gggagaagaa gatctcgtaa tgcgtcgtgg
     4801 ccggcaggat ggctagcctg gcatcggaca tcccggatcg gtcccatccg ccatcggccc
     4861 tgcctccgcc gagaagttcg aagagctcaa ccgcgtgcgc ggtgcggacg ctgtcggcgt
     4921 ctccgaccac gatcatcgtc ggtacctcca tcgccgccac gtcttccgac cagtcgtagg
     4981 cctggcgaag taagtcaccc aacttggtga gcagcaaggg ccaatcgtcg ggcctcggcg
     5041 cgataccgga atagagctgg tacatagggg tagctttcat cggctccgcg gcctcgggac
     5101 ccatctgctc cattcccgcg aggacctccg ggtaccagcc atccctcttg aaggtggtcg
     5161 agacgacgac cagcttgcgc acgacaccgg ggtgacggat ggcggtctgc aaggcgacgc
     5221 cgccgccaag cgagtagccc attacgtctg ctcgttcgat ctggaggtgt tcgatcaggg
     5281 ctgcgacatc atccgccata tgctcgtagg agagcgggcg accttctatg tcggctgtgc
     5341 gcccgtgcgc ctggagatcc acgcctatga tctgacgccc ttcagcgagc ataggcagga
     5401 cctcgccgaa catctcgata gcacccacgc cgccatgaag taatatcaac ggatccccgc
     5461 tgccgtggac ctcgtaatac atttcgagac cgttgacctc cgcgtagttg cccttattct
     5521 ccgtcatggc gttccgcctt ctctcgatat ccagggcaac ctcagacaga tcgccgttca
     5581 actgatgccc gcgtccatca aactcgcgaa aagatgcctg cgggaacctt tccttgtaca
     5641 tcgcaaggtg tacgaacggc acaacctcgt catcgcggct atggtaaaag aaagctggca
     5701 gctctccgcg gagctccgaa gcaaagtctt ccttcagttc gtactcgctt acctgccaat
     5761 cctcagcacc ccagtagggt gccgagatca ggaatatccc ggctataggc ttttccacct
     5821 tctcttcaga gagatacttc aacagaacag aaccgcccag agagtgtccg acgaggaaaa
     5881 cctccccgtc cagggcggtg agttcctgtg agatccgatc cctccacgcc gcgtactcgg
     5941 gactatgttc atcaggcatc tttggagaca gcacgtcgta cgcagctccc agcacgctct
     6001 gcagattttc cgccaatttc ttgtcttctt cgtgtgcccc ttctccgcca ccgtgaacga
     6061 acagcacctg tcccttttta gagatactct ctcccatggt tccctactcg accctggtca
     6121 tggtcgcctc atatccgccc cccggccact cccaacggcc ggttattgtg tccccattgt
     6181 cgccgaacat gcccttgaaa gccgccggag agcccacgaa tccaccccag atcgtgagcg
     6241 tgtcgtcgcc tacctcccac acgtactcaa gggcaactcc cccgaacgga ccaggacccg
     6301 tattgccgaa gaagtacgac ttcaagacct cgttcgactc gtcgtatcct atgtactcga
     6361 cacccttgat cctgttcccg atgtggtcaa ggtcaacgtg ttgaacgagg tagaagccgc
     6421 cttccatcca ctcgaaagta acccgtccgt gtatctctcc atccgggccg gattcacgcc
     6481 ccttcaggtc ccacgtgccg accatgacct caagactccc gagcgcggag ttcggctcgg
     6541 gcgcctgccg ttgagcgtct tgctccgcca tagcagcttc tccttccatc gggccgcgat
     6601 cgcctggaag gagcatacag caagagtcct aaaatctaac cagcaaatct tcaatcagga
     6661 cttgtaccgg tggaatccac gttatggcga gctctccgcc gctggggagg aatttgaggc
     6721 gagctatgag cgtgtggtgc ggctctacct ggcgttattc tcgcaatcgt cgtgtgaccc
     6781 gctcaagtag ccgggatcac gcactctgtc ttcgctctgc ggcggacttt gcccggtcgt
     6841 agctcaagcg gaagaggtcc acggctccgg gaacgtcttc cggctcccga acgcggtaac
     6901 tcacaacgcc cgcgaatccg gccgcgtgtt gcttcgcttt gccctcggag atcaggacgt
     6961 cgtggatctc ccttgggaac gcgatgtcgg ccacgccggt gtcgtggatg tgccctagcg
     7021 acctgcgacc gagcttgtag atggtcgcag gtggaacccg aaacccaccc cttcccgagc
     7081 ctccttcgtg tcgctccttg cttaccccgg gccagccgag cacctcgcgg tctaccatcg
     7141 ccagtagttc gtcgttcatc cgttgcctcc tcgttcgaaa gcatccccgt gcgttatgca
     7201 cagggtggca cacagggagg tgctgcgcat cgtgcgaaag cacggccttt acctgtacct
     7261 acgtacggtt tgtgtgaagc ccatgcaccc tgctgcctac aatagtcagt gccctgggta
     7321 ccacgacatc atccttgccc tccatgaccc tgtgccgtcc gttcgggtgc gcgtctgtgg
     7381 tttgcttgcc gatgtcgcta atccacgcgg ggtttgtcag aaagcagatc gatcaatata
     7441 gttcatttgg gtgatgcgct ggatactcga acgccagtaa agtactacgc gcgtgatagg
     7501 aatccacaag gggacatttc gcgaaccaat cagtaaaacg aacgtgagcc gcaaagagcg
     7561 ggaccatggg cagcccgttc cgtgaagaga cgttctcacg atttggacag acccaaatag
     7621 gtgctgctga ccaggaggag gccggatctt gaccatccca accaattacg aaccaatccc
     7681 gcaaccgcct ggatatccgg tgcttggcaa cctgttcgac ctgagagggg ctgataccgc
     7741 c
//
LOCUS       DATAIR010000828         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_177393, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000828 DATAIR010000000
VERSION     DATAIR010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177393"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VHM69_19635"
     CDS             complement(<1..1243)
                     /locus_tag="VHM69_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2742661.1"
                     /translation="MFSAKKSSSRKSTHLNPWRKARELLTPKSGKEFRPRLSMRIWLT
                     ALFVLVTALAAITAYRIVHPILYETLNRSSEAAFKQVGDQFEAQVQRLRDRDEALTEQ
                     RISAFARSHGLQWGIVKGEGGIQTQGNLDDWSASAVKRAIEEPRRPSDDMEQIETGVH
                     KGQRQATYAYPIDVEIEGKTEPRAVVFVKFFTKNDVENVESALRNIEHLALLAGGLAL
                     LIAGFAGYFAAVLISRRVSRLGLAAEHLAAGNFDERIQTRIEDEVGALGETFNAMAVS
                     LKGAFDQIEQEKERGSAILDGMTDAVIGVDRDLNAVFLNPRARKLLESSDHAFHNRLQ
                     EVLAKTRYSGPVTEPEARAGDRIIEIRAAPLEDGALAILRDVTEERHMQRVKAEFIAN
                     ASHELKTPLTALSGYLEMLEDE"
     gene            complement(1230..1907)
                     /locus_tag="VHM69_19640"
     CDS             complement(1230..1907)
                     /locus_tag="VHM69_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742662.1"
                     /translation="MTRKILLVDDEPSLQKMLTHALEREGFQIQVAGDGEAALEAFKT
                     FEPHLIVLDIMLPKLDGTEVIRRIRAQSDVPVIMLTARDDEIDRVVGLELGADDYVTK
                     PFAVRELVARVRAIMRRAAVPAGQRPDELTYDRLRINLPSRRVSADGEEVDLTYTEFE
                     LLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNPEFIHTAR
                     GVGYVFR"
     gene            complement(2025..3827)
                     /locus_tag="VHM69_19645"
     CDS             complement(2025..3827)
                     /locus_tag="VHM69_19645"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX2742663.1"
                     /translation="MSPSLYGFVKDHEPVSPEEISTEFLSLASQNGDARSIVERLVKD
                     DSRFCWDGEDLRTAHAGSLALEEAPYVVFDVETTGPAAGKGGAITEFGALKLVRGEVI
                     DEFSTLVNPGRPIDPFVVRLTGITDRMVSGAPPVSDVMPRFEEFVEGCVLVGHNVGFD
                     CSFVTAARGAPLPNEVIDTLKLARCLVPGLRRYRLSALVTHFGVRQAPNHRALSDAAA
                     TTDVFKKLLKLLRSAGIKSVGEAATIRGGRGRIKPQKQHLASGVPHTPGVYYFVDKHG
                     TILYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVAEVRVKETESELHALILEAREIK
                     RLLPRYNSVGRDDRASWFIRLDTNEPYPVPERVSTNPPDEGIIHLGPYRSAGGLDTCI
                     EALGRIFPLKRCSGEGEVCFYGQMGRCAPCVGMSEREYRSRVVDEVVALLRGEGGEEH
                     LRALVRERERLAAGLEFEAAARLRDLISGIERVRLSRAVVSAEGVQAVVAPSTEPGLI
                     EVFILCGGSLAAHSGFEPGDEAGLTSFAEKVLARPNGSRPEKEVSNEARIVAAYLRRR
                     QTEFEAVRLKEARDLLKAAERVAETAGGSASPLA"
     gene            complement(3817..>4216)
                     /locus_tag="VHM69_19650"
     CDS             complement(3817..>4216)
                     /locus_tag="VHM69_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2742664.1"
                     /translation="GEPPGHTTVRNANLLHPLPMTPSLMSRGVYYTEQIELFADTCRR
                     VLVDGEKLAGVGSLRIQRGKLFHTTKMSGVGMKTRARVLASGLLDDEYTYESLVAIPG
                     ITETQARTIAEAARKGSVPIGAAWTEVRVS"
BASE COUNT          667 a         1389 c         1331 g          829 t
ORIGIN      
        1 cctcgtcttc gagcatctcc aggtagccgg agagggccgt aaggggtgtc ttcagctcgt
       61 gggaggcatt agcgatgaac tcagccttca cgcgttgcat gtggcgctcc tccgtcacgt
      121 cgcgcaggat ggcgagtgcc ccgtcttcca gaggcgcggc ccgtatctcg atgatcctgt
      181 cccccgctct cgcctcgggt tcggtaaccg gcccgctgta gcgggtcttc gcgagtacct
      241 cttgcaggcg gttgtggaaa gcgtggtcgg atgactcgag aagcttcctg gcgcgagggt
      301 taaggaagac cgcgttcagg tctctgtcca cgccgatgac cgcgtccgtc atgccgtcga
      361 ggatggcgct accgcgctcc ttctcctgtt cgatctggtc gaaggctccc ttcagggaga
      421 cggccatcgc gttgaacgtc tcgcccagtg cgccgacctc gtcctcgatg cgcgtctgaa
      481 tcctctcgtc gaagttacca gcggccagat gctcggcggc cagccccaga cggctgacgc
      541 gccgggagat caagaccgct gcgaagtagc ccgcgaatcc cgcgatgagc agtgccagac
      601 cgccggcgag cagggccagg tgctctatat ttctcagcgc cgactccacg ttttcgacgt
      661 cgttctttgt gaagaacttg acgaatacaa ccgcccgagg ctcggtcttg ccttcgatct
      721 ccacgtctat gggataggcg taagtggcct gccgttgccc cttgtggaca cccgtctcga
      781 tctgttccat atcgtcgctc ggtctcctgg gctcctcgat ggcgcgtttc accgcgctgg
      841 cagaccaatc gtccaggttc ccttgcgtct ggatgccacc ttcccccttg actatgcccc
      901 actgcaatcc atgggagcgg gcgaacgcgc tgatgcgctg ttcggtcagg gcttcgtcgc
      961 ggtctctcaa ccgttgcacc tgggcctcga actggtcccc aacctgcttg aaggcggcct
     1021 cgctcgagcg gttgagggtc tcatagagga tgggatgcac gatcctgtag gccgttattg
     1081 cggcgagggc ggtcacgagc acgaagagcg cggtgagcca gatgcgcatg ctcaggcgag
     1141 gtctgaactc cttccccgac ttcggggtga gcagctccct ggccttccgc caggggttta
     1201 gatgcgtact cttccttgag gaactcttct tagcggaaaa catatccgac accccgcgcg
     1261 gtgtggatga attctgggtt cctggggtcg cgttctatct tctcgcggag gtggcggatg
     1321 tggacgtcca ccgtgcgttc gtcgcggaac tcgtcgcccc acaccctgcg caggagagcg
     1381 ctgcgcgaga acaccctgcc tgggctcgag gccagggtga cgagtagctc gaactccgtg
     1441 tacgtaaggt ccacctcttc gccgtccgct gacaccctgc ggctcggaag gttgatcctg
     1501 agcctgtcgt aggtcagctc gtcagggcgc tggccagcgg ggacggcggc cctgcgcatg
     1561 atcgccctta cgcgcgcaac cagttccctt acggcgaacg gcttggtgac gtagtcgtca
     1621 gccccgagct cgagcccgac caccctgtct atctcgtcgt ctcgggccgt cagcatgatg
     1681 acaggcacgt cgctctgcgc cctgatcctc ctgatgacct cggttccgtc gagcttgggg
     1741 agcatgatgt cgagcacgat caggtgcggc tcgaaggtct tgaacgcttc gagcgcggcc
     1801 tcgccgtcac cggcgacctg gatctggaac ccctcgcgct cgagggcatg tgtgagcatc
     1861 ttctgtagcg acggctcgtc gtctaccaga aggatcttgc gcgtcatgcc ctgtagatcc
     1921 tgtaccattc ccggctaaac ccttttctcg tacaccggaa ccgacaagtg acttgggttc
     1981 attttagcat gatggagaac acagcctgct gattggacgg ggcctcacgc aagcgggctc
     2041 gcactcccgc cggcggtctc agcgacccgt tctgcggcct tcaggagatc ccgcgcttct
     2101 ttgagacgca cggcctcgaa ctcggtctgc ctgcgcctga gataggccgc gacgatcctg
     2161 gcctcgttgg agacttcctt ttccggacga gacccgttgg gcctcgccag caccttctcg
     2221 gcgaaggacg tgagaccggc ctcgtcgcca ggctcgaagc cgctgtgggc ggcaaggctg
     2281 ccaccgcaaa ggatgaagac ctcgatcaag cctggttcgg tcgatggggc gacgacggcc
     2341 tgcacgccct ccgcgctcac gacagccctg gagaggcgca cgcgctctat gccggagatt
     2401 agatccctaa ggcgggccgc cgcctcgaac tctagccccg ccgcgagccg ctcgcgctcc
     2461 ctgacgaggg ccctgaggtg ctcctcgccg ccctcgccgc gcaggagggc gacgacctcg
     2521 tcaacgaccc gggagcggta ttcccgctcg ctcattccca cgcacggcgc gcagcgtccc
     2581 atctggccgt agaagcagac ctccccttcg cccgagcacc gcttgagagg gaagatcctt
     2641 cccaaagcct ctatgcaggt gtcgaggccc cctgcgctcc tgtacggtcc gaggtggatg
     2701 atgccctcgt ccggcgggtt cgtggagacc cgctcgggga cggggtaggg ttcgttggtg
     2761 tcgagcctta tgaaccagct cgccctgtcg tccctgccga cggagttgta gcgtggcagc
     2821 aggcgtttta tctccctggc ctcgaggatg agtgcgtgaa gctcgctctc ggtctccttc
     2881 acgcgtactt cggctacttc ctcgacgaga cgcccgacct tgcgacgccc gtccccgccg
     2941 ttgaagtagg tccgcacgcg ggccttcagg tccttcgcct tgccgacgta gaggatagtg
     3001 ccgtgcttgt cgacgaagta gtagacgccg ggcgtgtgcg gcaccccgct tgcgaggtgc
     3061 tgcttctggg gtttgatgcg gccccttcct ccgcggatgg tggcggcctc cccgacgctc
     3121 ttgatcccgg cggagcgaag gagcttgagc agtttcttga acacgtccgt cgtcgcagcc
     3181 gcgtctgaga gggccctgtg gttcggcgcc tggcgcacac cgaagtgggt gacgagcgcg
     3241 ctcagacggt aacgcctgag acccggtacc aggcaacggg cgagcttcag tgtgtcgatg
     3301 acctcgttgg ggagcggagc ccccctcgcg gcggtgacga aggagcagtc gaacccgacg
     3361 ttgtgcccga caaggacaca cccctcgacg aactcctcga agcgcggcat cacatcggag
     3421 acggggggtg cgcccgaaac catgcggtcc gttatgcccg tcaggcgcac gacgaaggga
     3481 tctatgggcc gcccaggatt gacgagcgtc gagaactcgt ctatcacctc cccgcgtacg
     3541 agcttgaggg cgccgaactc cgtaatcgcg ccgcccttgc ccgccgccgg tccggttgtc
     3601 tccacgtcga agacgacgta gggagcctcc tccagggcga gcgagccggc gtgcgctgtc
     3661 cgcaggtcct cgccgtccca gcagaaccgg gagtcgtcct tcacgagcct ctccacgatg
     3721 ctgcgggcgt cgccgttctg gctcgctaga gagaggaatt ctgtggaaat ctcctccggg
     3781 ctcacaggct cgtggtcctt cacgaagccg tagagactag gagacacgga cctccgtcca
     3841 cgccgccccg atcgggacag atcccttcct cgcggcctcg gcaatggtcc tggcctgcgt
     3901 ctccgtgata ccggggatgg cgacgaggct ctcgtaggta tactcgtcgt cgagcagccc
     3961 gctcgcgagg accctggccc gcgtcttcat ccctacaccg gacatcttcg tcgtgtggaa
     4021 gagctttccg cgctggatgc gcagagatcc gacgccggcg agcttctccc catcaacgag
     4081 gacgcgcctg cacgtgtcgg cgaagagctc gatctgctcg gtatagtaga cgccccggct
     4141 catcagggaa ggtgtcatcg ggagagggtg cagcaggttc gcgtttcgga cggtcgtgtg
     4201 accgggaggc tcgcct
//
LOCUS       DATAIR010000829         8452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_177630, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000829 DATAIR010000000
VERSION     DATAIR010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177630"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VHM69_19655"
     CDS             complement(<1..744)
                     /locus_tag="VHM69_19655"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX2742665.1"
                     /translation="MRRTGIKEKIEAAFEDRALLEEEEYRTAVFDTVAALDRGELRVA
                     QKSDGEWRSNAWVMKAINLYFSVARMQTTEVGPFEYHDKIPTKKNLADAGVRVVPPGT
                     VRYGAFVEPGVVVMPGYVNIGAYVGSGTMVDTWATVGSGAQIGRDVHLAGGVGIGGVL
                     EPPGAMPVVIEDGAFVGSRCIVVEGVRVEEGAVLGANVVLTGSTQIIDVTGPDEVVHK
                     GRVPARSVVVAGTRTKQFPAGDYQLQSALI"
     gene            780..1688
                     /locus_tag="VHM69_19660"
     CDS             780..1688
                     /locus_tag="VHM69_19660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2742666.1"
                     /translation="MIPAILPSARERSRYLMTFFALVLILAAAVFHATWNLLAKRVGD
                     GGPVFVWLFGICSVLIYAPLAVVVVFVNAPQLGPVQLLFMFGSGVLHLGYFVLLQRGY
                     AVGDLSLVYPLARGTGPLLATAAAIVLFGERPSLLAIFGIGLITAGVFVLTSESGSLR
                     SGFGMGVVYGLLTGVFIAAYTIWDKQAVSALMIPPLLQSWATTLIMALLLSPLAMNRR
                     KEALALWRAHKPAILGVAVLSPLSYILVLTALVFTPVSYVAPAREISILIGAAMGTHL
                     LSEGDSTRRLIAAGAMVAGIIALALG"
     gene            complement(1692..2183)
                     /locus_tag="VHM69_19665"
     CDS             complement(1692..2183)
                     /locus_tag="VHM69_19665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2742667.1"
                     /translation="MRDATLEDLASIVEIYNSTIPSRIVSADTQPVSVEQRLPWFREH
                     DPSRRPILVAEEGGEVVGWISLSDFYDGRPAYHSTAEIGIYVREGHRSRGIGGRMLEE
                     TIRRAPDLGIKTLTAGAFAGNEPSLRLFERFGFQRWAHFPRVAELEGTEQDLVVLGRR
                     LTE"
     gene            complement(2186..3382)
                     /gene="dapC"
                     /locus_tag="VHM69_19670"
     CDS             complement(2186..3382)
                     /gene="dapC"
                     /locus_tag="VHM69_19670"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX2742668.1"
                     /translation="MKPGATPPANETSKPTPPLLLNPVLAQQGAYPLLRLDERRQELE
                     EKGVDLFDFGTGDPREPTDGRIRQALIDGVPEVSRYPSTPGKASLREAFCGWMRRRHG
                     VALDPESDVLPATGAKEAIFHAPLAFLHHSHARRGVAYGTPGYPVYERGTHYAGGEAL
                     PVELKREDGFLLPTEAIDPERTRILWINYPHNPTGAPANYEYLEEVVSFCQEHDILLF
                     SDECYNEIYSGEPPPSILEITTERTLAFCSLSKRSGMTGYRSAMMAGDPELISALKKL
                     RPSIGVATQYFVQDAATAAWSDDEHVEKRRRIFGEKRALFTEFFEKAGLEYLSTDASF
                     YLWVAVPDGDDEAYALKLMDEGIIVAPGRSFGRGGEGYVRIALVPEIEDCRVAIERWG
                     ELEART"
     gene            3463..4713
                     /gene="lysA"
                     /locus_tag="VHM69_19675"
     CDS             3463..4713
                     /gene="lysA"
                     /locus_tag="VHM69_19675"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2742669.1"
                     /translation="MDDFHYEGGGLRCEEVPLSEIAGAAGTPAYIYSHAALERAYREL
                     DDAFSGLDHLVCYAVKANGNLAVLRALASLGAGADIVSGGELYRVMRAGFDPKKVVFA
                     GVGKTERELMSGLGERILLFNLESASELEHLDRICARFGKRARVALRVNPDVDAQTHD
                     HVATGRGSDKFGIPVDEALALAERIGGYRCIDLIGVHQHIGSQITKLAPYTESVERSA
                     SLVDELKTRGFDIRYFNIGGGLGIPYKDEETPTPKELVDAIRPMLEATGTKILCEMGR
                     YIAGAAGVLLTSVVYRKKSGGKSFIVADAGMNDLLRPSLYDAHHEVLAVNEDGAVANA
                     DLVGPVCESGDYLARDRELPDASEGDVLAIMDAGAYGFSMASNYNSRPRPAEIMVRGD
                     SWAVVREREHNADLVKGELVPAFL"
     gene            4727..5485
                     /locus_tag="VHM69_19680"
     CDS             4727..5485
                     /locus_tag="VHM69_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742670.1"
                     /translation="MVVTVPEGLRIFTLAERPDLESKVRSLPDMFPEFMYHDVVVNRY
                     WDSLFTDFAGFQIAVCEGGDEIVAAGHCIPVFWDGTAETLPGGLDEVLERGVGEERAQ
                     TVVSALLALVPHAHRGRGLSSVVLGAMKSVAAEHGLGAMIAPVRPTLKDCYPLTPMER
                     YIRWERPDGLPFDPWLRVHSRLGAQFLRVAPRSMVITGTIAEWEEWTGMYFPESDAYV
                     VPGALSPVAMELERDLGSYEEPNVWMRHPVMVEE"
     gene            5503..6009
                     /locus_tag="VHM69_19685"
     CDS             5503..6009
                     /locus_tag="VHM69_19685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2742671.1"
                     /translation="MTLRSVEREDLETLWRFWNDLDVELAGGGDPPLPISLERMRARF
                     EREARETDRDKTDFVIEADGACIGHCGLFDIDTAARHCELGITIGETEYWGRGYGREA
                     VRLLLDYAFRVRNLRRVWLEVHATNERAIRAYKACGFIEEGRMREHVWLAGRYVDNVI
                     MGVLREEG"
     gene            6010..6924
                     /locus_tag="VHM69_19690"
     CDS             6010..6924
                     /locus_tag="VHM69_19690"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX2742672.1"
                     /translation="MAGGTHEAVPDARNEAVLVYINGEFFGREEAKISVFDSGFLVGD
                     GVWEGIRLHEGEFAFLDLHLDRLFEGAKSIDLDVGMTREEITSALRETVRRNGMHTGV
                     HVRLMVTRGDKKTPSQDPRLTIGGPNVVIIAEHKVADPGVKERGVRLFTSTVRRPPPD
                     TLDQRLNCHSKLHEVIALVQALKAGADEALMLDPTGAVATCNATNFFIVRSGEVLTST
                     GHYSLNGITRRIVIELSRAENVPVFERAFSLTDVYGADEAFVTGTFGGITPVIEVDGR
                     TIGTGERGELTSRLTELYAEAVAAATME"
     gene            6951..7676
                     /locus_tag="VHM69_19695"
     CDS             6951..7676
                     /locus_tag="VHM69_19695"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742673.1"
                     /translation="MWSGPRNVSTALMYSFRQRADTAVVDEPLYGHYLRSTGADHPGA
                     REVMETIELDGERVVREVILGPCERAVHFFKNMAHHLPGLDPSFMSDITNVLLIRDPK
                     EMLPSLTKQLPSPILRDTGLREQAGILDSILARGKEPIVLDARELLLDPRGVLRQTCS
                     RLGIPFEEAMLSWPAGPKPEDGVWAKHWYHNVHSSTGFAPYRPRTGPFPERLRPLLAE
                     CLPLYERLRYYAIVAGAAKSGGD"
     gene            complement(7657..8139)
                     /locus_tag="VHM69_19700"
     CDS             complement(7657..8139)
                     /locus_tag="VHM69_19700"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HEX2742674.1"
                     /translation="MVWVRVLIGAVWLNGGLEKLLNPDFPRQFAASLQAGGFVSQAPP
                     SFQDFMKGYVIPNAELFAQLMRAGELTLGIVLILGLLTNLAAIGSIGLSAMILLSQGG
                     VRLGTGLGAPEFLTINVLVALISLVILLSPAAKNVSVDSRLAQRSPGLSLFLINRRQT
                     "
BASE COUNT         1476 a         2682 c         2720 g         1574 t
ORIGIN      
        1 tatcagggca ctctggagct gatagtcgcc ggcggggaac tgcttggtac gggtccctgc
       61 cacgacgacg gagcgcgcgg ggacgcggcc cttgtggacg acttcgtcgg gacctgtaac
      121 gtcgatgatc tgggtcgagc ccgtgagcac gacgttcgcc ccgagtacgg ccccctcctc
      181 gacgcgcacc ccttcgacca cgatgcaccg cgagccgacg aaagcgccgt cttcgatcac
      241 gaccggcatc gcgccaggcg gttcgagcac cccgccgatg ccaacgccac cggccagatg
      301 cacgtcgcgc ccgatctggg cccccgagcc gaccgtggcc cacgtatcca ccatcgtccc
      361 gcttccgacg taggccccga tgttgacgta gccgggcatc acaacgacgc cgggctcgac
      421 gaaggcaccg tagcgcacgg taccaggcgg cactacgcgt acccccgcgt cagccaggtt
      481 cttcttcgtg gggatcttgt cgtggtactc gaaaggaccg acctcggtcg tctgcatccg
      541 ggcgacggag aagtacaagt taattgcctt catcacccag gcgttggatc tccactcgcc
      601 gtcgctcttc tgcgcgaccc gcaactcgcc cctgtccagg gcggctacgg tgtcgaagac
      661 tgcggtgcgg tactcctcct cttccagcag tgcccggtcc tcgaacgcgg cctctatctt
      721 ctccttgatc cctgtcctcc ttatctcgac acgtgcgcca gtttagcagg aggccgggcg
      781 tgatccccgc tatactgcct tcggcccgtg agaggagccg ctatctcatg actttcttcg
      841 cgcttgtact gatactggct gccgccgtct tccacgcgac ctggaacctg ctcgccaagc
      901 gggtcgggga cggaggtccg gtcttcgtct ggctcttcgg catctgctcg gtgctgatct
      961 acgctccgct ggccgtcgtg gtggtgtttg tgaacgcgcc ccagttgggt ccggtacaac
     1021 tcctgttcat gttcgggagc ggcgtcttgc atctcgggta cttcgttctc ttgcagcggg
     1081 gatacgcggt cggggacctc tcgctcgtct atcctctcgc gcgcggcacg ggacccctgc
     1141 tcgcgacggc tgcggccatc gtcctgttcg gcgagaggcc gtccttactg gcaatcttcg
     1201 gtataggtct gatcacagcc ggcgtcttcg tgcttaccag cgaatcgggg agtcttcgct
     1261 ccggcttcgg gatgggcgtg gtctacggtc tactgaccgg cgtatttatc gcggcgtaca
     1321 ccatctggga caagcaggcc gtcagcgcgc tcatgatccc acccctcctc cagagctggg
     1381 ccacgaccct gattatggct ctcctgcttt cccccctcgc gatgaaccgc aggaaggagg
     1441 ctctggccct gtggcgcgcc cacaaacctg cgatcttggg agtcgccgtc ctcagccctc
     1501 tttcctacat cctcgtcctc actgctctcg tcttcacccc cgtgagctac gttgccccgg
     1561 cgcgtgagat ctccatcctc atcggtgccg ccatggggac acatctactc tcggaaggag
     1621 actccacgcg acgcctaatc gccgcaggcg cgatggtggc ggggatcatc gccctggcgc
     1681 tcggctgata actactctgt gagcctgcgt ccgaggacga cgaggtcctg ctccgtgcct
     1741 tcgagctccg cgacgcgtgg gaagtgggcc cacctttgga agccgaaccg ctcgaacagc
     1801 ctgaggctgg gctcgttgcc ggcgaaggcc cccgcggtaa gggtctttat gccaaggtcg
     1861 ggagcacgcc tgatggtctc ttccagcatg cgtccgccta tcccccggct cctgtgaccc
     1921 tccctgacgt aaatgccgat ctcggcggtg gagtggtacg ccggcctgcc gtcgtagaag
     1981 tcgctaaggc tgatccagcc tacaacctct cctccttctt cggccacgag gatcggacgc
     2041 ctcgaagggt cgtgctcgcg gaaccacggc agcctctgct cgacggagac gggctgggtg
     2101 tcggcactca ctatccggct ggggatcgtg gagttgtaga tctccactat ggacgcgagg
     2161 tcctccagcg tggcgtcgcg catcctcacg tccgtgcttc gagctcaccc caacgctcga
     2221 tcgccacccg gcaatcctcg atctccggca cgagggcgat cctgacgtac ccctcgccac
     2281 cgcgcccgaa agagcgtcca ggcgccacga tgatgccctc gtccatgagc ttcagagcat
     2341 aagcctcgtc gtccccgtca ggtacggcga cccacaggta gaagctcgcg tcggtggaca
     2401 gatactcgag gccagccttc tcgaagaact ctgtgaagag cgcgcgcttc tcgccgaaaa
     2461 tgcgccgacg cttctcgaca tgctcgtcgt cgctccaggc tgcggtcgcc gcatcctgca
     2521 cgaagtactg ggtggcgacg ccgatggagg gacggagctt cttaagggcg gagatcagct
     2581 ccgggtcgcc ggccatcatc gcggagcggt agcccgtcat gccgctgcgc ttggaaaggg
     2641 agcagaaggc gagggtccgc tcggtggtga tctcaagtat ggatggaggc ggctcgcccg
     2701 agtagatctc gttgtagcac tcgtcggaga agagcaggat gtcgtgttct tggcagaacg
     2761 aaactacttc ttccaggtac tcgtagttgg ctggtgcgcc tgtgggattg tgtgggtagt
     2821 tgatccagag tatccgcgta cgttcgggat ctatggcttc tgtcggtagc aggaaaccgt
     2881 cttctctttt taactccacg ggcaacgcct cgccgccggc gtagtgcgtc cctcgctcgt
     2941 agacgggata gcctggcgtg ccgtaggcga cgccgcgcct ggcgtgcgag tggtgcagga
     3001 aggccagggg agcgtggaag attgcctcct tcgcgcccgt ggccggcagc acatcgctct
     3061 cggggtcgag cgcgacgccg tgacgccgcc gcatccagcc gcagaaagcc tcgcgcaagc
     3121 tcgccttgcc cggcgtgctc gggtaacggc tgacctcggg gacgccgtct atcagagctt
     3181 gacggatcct gccgtccgta ggttcgcgtg gatcgcccgt gccgaagtcg aagagatcta
     3241 cgcctttctc ctcgagttcc tggcggcgtt cgtcgaggcg caggagcggg tatgcgccct
     3301 gctgcgcgag gacggggttg aggagtaggg gcggagtggg cttgctggtc tcgttcgcgg
     3361 gtggtgtcgc gccgggcttc atcacggagt atattctccc tacggctcca attgcaggag
     3421 ccagaaaata tagcacgctc cggctacacg aggaggtacg ttttggacga ctttcactac
     3481 gaggggggcg gactccgctg cgaggaagta ccgctctccg agatagccgg cgccgccggc
     3541 acgcctgcct acatatacag tcacgccgcc ctcgaaagag cctatagaga gctcgatgac
     3601 gcattttcgg gcctggatca cctggtttgc tacgcggtca aggcgaacgg gaacctcgcc
     3661 gtcctgcggg cgctcgcttc tttgggggcg ggtgcggata tcgtgtcagg aggagagctt
     3721 taccgggtga tgcgcgccgg gttcgacccg aagaaggtcg tcttcgcggg ggtcgggaag
     3781 accgagcggg agctcatgtc gggcctgggg gagcgcatcc tgctcttcaa cctcgagtcg
     3841 gcctccgagc tggagcacct cgaccgcatc tgcgcccgct tcggcaagcg ggcccgcgtc
     3901 gccctgcgcg tcaaccccga cgtggacgcc cagacccacg accacgtcgc cacaggaagg
     3961 gggagcgaca agttcggcat ccccgtcgac gaagcactgg ccctggctga gaggatcggg
     4021 gggtacaggt gcatcgacct catcggggtc caccagcaca tagggtccca gatcaccaag
     4081 ctcgcccctt atacggagtc ggtcgaacgt tccgcgagcc tcgtagatga actcaagacg
     4141 cgcggcttcg acatccgcta cttcaacatc ggcggcggcc tcggcatccc gtacaaggac
     4201 gaggagaccc cgaccccgaa ggagcttgta gacgccattc ggcccatgtt ggaagcgacg
     4261 gggaccaaga tcctgtgcga gatgggccgc tacatcgccg gcgccgccgg agtcctcctg
     4321 accagcgtcg tatatcgcaa gaagagcggc gggaagagct tcatcgtcgc ggacgcagga
     4381 atgaacgacc tcctgcgtcc tagcctctac gacgcccacc acgaggtcct cgccgtgaac
     4441 gaggacggcg ccgtcgctaa cgcggacctc gtcgggcctg tgtgcgagtc gggcgattac
     4501 ctcgcccgcg accgcgagct gcctgacgcc tcggagggcg acgtgctcgc catcatggac
     4561 gcgggcgcat acgggttctc gatggcctcg aactacaact cccgacccag gccggccgag
     4621 atcatggtca ggggagatag ctgggccgtc gtgcgcgagc gcgaacataa cgcggacctc
     4681 gttaaaggcg agctggttcc ggcgttttta taggcttcag gtctgcgtgg tcgtgacggt
     4741 gcccgagggt ttgcggatct tcacgctggc cgagaggccg gatctcgaat cgaaggtgag
     4801 atccctgcct gatatgttcc ccgagttcat gtaccacgac gtggtggtca atcgctactg
     4861 ggacagcttg tttacggatt tcgccgggtt ccagatcgcc gtctgcgagg gtggagatga
     4921 gatcgtcgcc gcggggcact gtatccctgt gttctgggac gggactgccg agacgttgcc
     4981 gggagggctg gatgaggtgc tggagcgagg ggtgggagag gaacgtgctc agaccgtggt
     5041 ctcggccctc ctggcactcg tgccgcacgc ccatagggga cgcggtctca gcagcgtggt
     5101 cctcggcgcg atgaagagcg tggccgccga acacgggctc ggagccatga tcgcccccgt
     5161 ccgacccacg ctgaaagact gctacccgct cacccccatg gagcgctaca tacgctggga
     5221 gcggcccgac gggctccctt ttgacccctg gctgcgggtt cacagcaggc tcggcgcgca
     5281 gttccttcgt gtcgcgccaa gatccatggt catcaccggc accatagcag agtgggagga
     5341 gtggacaggg atgtatttcc ccgagagcga tgcatacgtc gtgcccggcg ccctgagccc
     5401 cgtcgcgatg gagttggaac gggaccttgg ctcgtacgaa gaaccgaacg tctggatgcg
     5461 ccaccctgtc atggtcgagg aataatgctc acgggagaga aggtgacact gcggtccgtg
     5521 gagcgcgagg acctggagac cctgtggcgg ttctggaacg acctcgacgt ggagcttgcg
     5581 ggaggcggcg acccgccgct tccgatctct ctcgaacgga tgcgggcgag gttcgaacgc
     5641 gaggcccgtg agaccgatcg tgacaagacc gacttcgtaa tagaggccga cggcgcgtgc
     5701 atcggacatt gcggtctctt cgacatcgac acggctgccc gtcactgcga acttggcatc
     5761 accatcggcg aaacagagta ctggggacgc ggctacgggc gcgaggccgt gaggttgctg
     5821 cttgactacg cgtttcgtgt aaggaacctg aggcgcgtgt ggctcgaggt ccacgcgacg
     5881 aacgagcgtg cgatccgggc ctacaaagcc tgcggcttca tcgaggaagg caggatgcgc
     5941 gagcacgtct ggctcgccgg acgctacgtc gacaacgtca tcatgggcgt actgcgcgag
     6001 gagggatgat tggccggggg gacgcacgag gcagtgcctg acgcacgcaa cgaggcagtc
     6061 ctcgtgtaca tcaacggtga gttcttcggg cgggaggaag cgaagatctc cgtcttcgac
     6121 tcgggattcc tggtcgggga cggagtctgg gagggcattc gtctgcacga aggagagttc
     6181 gctttcctgg atctacacct ggatcggctc ttcgaaggtg cgaagtcgat agaccttgac
     6241 gtcgggatga cgcgcgagga gatcacctcc gccctgcgcg agaccgtccg ccgcaacggc
     6301 atgcataccg gcgtccacgt gcgcctgatg gtcacacgcg gggacaagaa gaccccgtcg
     6361 caggacccgc gtctcacgat aggcggtcca aacgtcgtca tcatcgccga gcacaaggtg
     6421 gccgacccag gggtgaaaga acggggcgtc aggctcttca cctcgacggt gcgccgcccg
     6481 ccgccggata cgttggatca gaggctcaac tgccacagca agctgcatga ggtaatagcc
     6541 ctggtgcagg ccctcaaggc cggagcagac gaggccctga tgctcgaccc gaccggcgct
     6601 gtggcgacgt gcaacgccac caacttcttc atcgtgcgaa gcggcgaggt gttgacctcg
     6661 actggtcact actccctgaa cggcatcacg cgccgcatcg tcatagaact cagccgtgct
     6721 gagaacgtac ccgtcttcga gcgggccttc tctctcaccg acgtgtacgg cgcggacgag
     6781 gcgttcgtca caggcacctt cgggggcatc acgcccgtta tcgaggtgga cggacggacc
     6841 atcggtacgg gcgagcgcgg cgaactgacc tcccgcctta cggaactgta cgccgaggcg
     6901 gtcgccgcgg cgacaatgga atagcagtgt cggagtgtct gaggttgtct gtgtggtcgg
     6961 gaccgcgcaa cgtctcgacg gctctcatgt actccttcag gcagcgggcg gataccgcgg
     7021 ttgtggacga gcctctctat ggacactacc tgagatcgac cggcgcggat catcccggcg
     7081 ccagggaggt catggagacg atagaactgg acggcgagcg ggtggtgcgt gaggtcatcc
     7141 tcggtccttg tgagcgtgct gtccatttct tcaagaacat ggcgcaccat ctccccggtc
     7201 tcgacccgtc cttcatgagc gacattacga acgtgctcct gatccgtgac cccaaagaga
     7261 tgcttccttc attgacgaag caacttccct ccccgatcct gcgggacacg ggactgcggg
     7321 agcaggccgg tatcctcgac tcgatcctgg cccgcggcaa agaacccata gtactcgacg
     7381 ccagggagct gctcctcgac ccgcgtggcg tactgcgcca gacttgctcc aggctcggca
     7441 tacccttcga ggaggcgatg ctctcctggc ccgcaggtcc caaacccgag gacggcgtgt
     7501 gggccaaaca ctggtaccac aacgtccact cctcgacagg tttcgcgccc tacaggccac
     7561 gtaccgggcc gttccccgag cggctgaggc cgctacttgc ggagtgcctg cctctctacg
     7621 aacgtttgcg gtactacgcc atagtcgcag gagccgctaa gtctggcggc gattgatcag
     7681 aaatagcgag agtccggggc tgcgctgtgc gaggcgggag tccaccgaga cgttcttcgc
     7741 tgcggggctt agcaggatca ccagggagat cagggccaca agcacgttta tcgtgaggaa
     7801 ctcgggtgcg ccgaggcccg tcccaagcct cacgcctccc tgggagagca ggatcatggc
     7861 gctcaacccg atgctgccga tggcggcgag gttggtgagc agaccgagga tgaggactat
     7921 cccgagggtc agttctcccg cgcgcatgag ctgggcgaag agctcggcgt tggggatcac
     7981 gtatcccttc atgaagtcct ggaaggacgg cggagcctga gagacgaagc ccccggcctg
     8041 caggctcgct gcgaactgcc gtggaaaatc cgggttcagc agcttctcca ggccgccgtt
     8101 gagccatacg gccccgatca ggacgcgcac ccacaccatg ccgttcacag gtcggactcc
     8161 tctcacgggt tggcgagtct tttaatcata tacgcagccg tggacctgtg ggatgccgca
     8221 cagaacaccc acgcacacaa atgcggatcg aagggcgctt ttcgcatgta ccaactgtcg
     8281 cgctggccac cgcggttttc attcgagggg tggcgagggc tccgccgcga tcatcgtgtc
     8341 ctgatagaac ctgtaatcgg cacctcctct acgacttctc aatctcgggc gccccatggt
     8401 agaaccggag tcttagacca tgcgtatatc atgggtaccc ttagagacga gg
//
LOCUS       DATAIR010000830        16554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_84359, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000830 DATAIR010000000
VERSION     DATAIR010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84359"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VHM69_19705"
     CDS             complement(<1..577)
                     /locus_tag="VHM69_19705"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX2742675.1"
                     /translation="MARWRIVLPLMVLLLAAVLLAVPSIRSELPGMTHRATYTNPVIR
                     WDFADPTVLKADDGWYYAYSTENLTIERLAYIQAARSRDLVNWELLPDALPEKPEWAS
                     TTRDFWAPGVIEADGRYYMYFSGVHDERESMCLGVATSGSPGGPFVPEDEPLRCGDGF
                     ENIDPMPFDDPRTGESLLYWGSDGAPIRVQRL"
     gene            complement(570..1052)
                     /locus_tag="VHM69_19710"
     CDS             complement(570..1052)
                     /locus_tag="VHM69_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742676.1"
                     /translation="MRALGIALLVVLTAEVCLLALALGPPDVTFPADSDLPMEQRVNG
                     WQRAAELARTDTFEPASLAGGERPELTVRGIPGDSSRPLAAEYGDGLVIRQAHVDVDP
                     QLEDEPAEVEGADNAWWGTVRDETYLYVVRGETLVILSGLPREDLIQTASSLRPVENG
                     "
     gene            complement(1075..1956)
                     /locus_tag="VHM69_19715"
     CDS             complement(1075..1956)
                     /locus_tag="VHM69_19715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2742677.1"
                     /translation="MAQHTERKEIRAPRFPRLPRLAHAAFVYALLVLASLFILLPVGW
                     MLTAALKPDTAPVFTFPPEWFPTEYWHWQTFHDALFKEKFIRYGLNSAFLVVMNVIGV
                     VLSSSLIAFPFARLRFRGKNVLFTFLVATMLLPAPVLLIPQFLLFYRIGWYGTYYPLW
                     VPAFAGNAFFIFLMRQYMRSIPIELDEAARVDGAGYWTIYWRIIMPLCIPVLTVAAVF
                     QFMWTWNDFFGPLIYLDDPEKFTVALAIATMVHRVGTEWNEVMAANLLVMLPVLIVYF
                     FAQDKLIGGIANVGIKG"
     gene            complement(1958..2923)
                     /locus_tag="VHM69_19720"
     CDS             complement(1958..2923)
                     /locus_tag="VHM69_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2742678.1"
                     /translation="MSAPTQQLGTQQVSRPRRSAPARAEERTAYLFLLPWFAGLLFFL
                     AIPLAYALYISMTDKRLISAGREHFVGLSNYVYMFTQDGFFYHSLYITLKWIFLTTPL
                     FLAAGLLLSLLLNQKYFGMNVFRTILYIPAVVSAVAVAVLWLQLLNPELGAVNYVLIK
                     LGVDNPPYWLADPQWAMPAVGLMSLWGVGGMAIIYLAGLQNIPPHLYEAATIDGAGAW
                     GKFRHITLPMLSPTIFFLLLNAVIDALLIFGPLFVLTLRGSEGLGGPDDSLLFYMVYL
                     YDVAFDQGFIGYGTALAWILTIIGVIMVLIMLRAERHFVFYESEE"
     gene            complement(2964..4376)
                     /locus_tag="VHM69_19725"
     CDS             complement(2964..4376)
                     /locus_tag="VHM69_19725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2742679.1"
                     /translation="MQGQHRISRGHFLRLSAMMAGGAMLAGCNLDEGGGPPGAKKGGG
                     GGEVDIGKIQAGENSELTKLAENWKPYDGPDVELSIWMYAQDPASMKAYKKAFEKEYS
                     NIKLKYVTYPEENYVTKVNVALQAHNPPDVAVMEERAWMKAHLTADLAPFYKAWGISV
                     DDFAPGGVARFTLEGGPEQGVFGVGDFLGGNIIVYNKDLFDEAGAEYPPTDRSLEWPG
                     YADLARAVAQPNSDPTKRVYGASAPEWGFGIWQRWVFGPDGRKILGNLNTEPEIEAWN
                     LGTALVRDKLAPSSQVTSTVAEPDLFTQKRIAMTWTDFTFMQDYEAANINFGIAPWPV
                     ISGSESFVDTWTAPWGTFVESEHLHAALTFLQFMGTEAQRIRAEVSSDPPLSLSVARE
                     IDWSKGDPIKEEYLSVLEVAAQAQPFVPPMPEGAYASVEVYNEMTVQGKTDAEPILDA
                     AAKKTQPILDKAWKDWEALA"
     gene            4617..5729
                     /locus_tag="VHM69_19730"
     CDS             4617..5729
                     /locus_tag="VHM69_19730"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX2742680.1"
                     /translation="MPRELIALEPRMPVLREYEEPELRLRQVRIRTEFASPKHGTEMV
                     GFRNEPSARRPYDAEWGCVIPREAGEGPRNFPLGNMAVGVVTEVGPEATRLRVGDRVF
                     GHLSIRETHTVDEDYVDPLPDALEAESAVCLDPAVMALAMRDAEIRLGDQVAVFGLGA
                     IGLMAVQMARIAGADMVIGVDPIEARREVAEALGADVTLDPRENDGDAGMSIRRISGA
                     TLGEVERPEGKRVLGGYWEVPTQYGQRGVDVAIEASGSVRALQQAIRATRFGGTICMI
                     SFYGGDAAGLRLGDEFHVNRQQLVSARVESLPLRDAPAWTLERLVEVGLRWLASGRLR
                     TEGIVTPVVPFEESVEAYREIDEHPERSIKLGISFD"
     gene            5785..6285
                     /locus_tag="VHM69_19735"
     CDS             5785..6285
                     /locus_tag="VHM69_19735"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2742681.1"
                     /translation="MPHEGQWHDVFERERNSILDHSAGLVIDVQHVGSTAVPGLDAKP
                     IIDIAVGVDTPRLVAVLSRILTGIGWIYRGDKGQDGGHLFVKEAAPDFRTHHLHVVVI
                     DDGRWKDYLAFRDILRRNERVRNEYAALKRELEKMYRDDRGSYTSRKSEFVSAVLAKH
                     AGKREA"
     gene            complement(6366..7340)
                     /locus_tag="VHM69_19740"
     CDS             complement(6366..7340)
                     /locus_tag="VHM69_19740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2742682.1"
                     /translation="MTTRFGVFVPQGWRMDLAEIEDPIEQYETMTSVARAADEGGWDS
                     IWVFDHFHTVPTPELETCFEAWTISATLARDTQNVKIGQMVGCNGYRNPALYAKIAST
                     VDVASHGRLYAGIGAGWYEHEWRAYGYGFPELRDRMGMFREACEIIHRMWTEDYPKFE
                     GKYYTIDGPINEPKGVQKPHPSFWIGGGGERVTLKLVAQWGDACNVAGSVPADIETIS
                     HKLDVLKRHCDDLGRDYDEIIKSTSVDIHVVDDMAGAEQATAAARGSRSFDDYATGTI
                     VGTPEMVRERLQPVIDAGIDYFIVTIPREAYDHDAVRRFAREVIPLFD"
     gene            complement(7361..8146)
                     /locus_tag="VHM69_19745"
     CDS             complement(7361..8146)
                     /locus_tag="VHM69_19745"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2742683.1"
                     /translation="MKLALREEMSRGGTLAEKLAWLEGIGFDGIELAAASLDLPQREL
                     EETFAGSPVRAANVAGSTALIDPDPAERSAAKDLMRRRLHLAAALGAVGVLTVPQFGS
                     APRLPDLSPYKTAADLERELFVEQLEELAPAVRDAGVPIFLEPLNRYEQHLVNRLEQG
                     VAFAEQVEGVKIMADFFHMNIEEADIAASIRAAGPHIVHVHVADSQRLQPGRGHLDFR
                     PGFAALKEAGYDGYLGIECGVAGPYEESLVECAGLLRELWDAA"
     gene            8350..9357
                     /locus_tag="VHM69_19750"
     CDS             8350..9357
                     /locus_tag="VHM69_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2742684.1"
                     /translation="MLVPRFLGGGEIGFAPKSVPEPGSGQLLIQVKANALCGSERWQY
                     FDGSGVTPGHEAAGIVVKGGPQTRTPLDTPGVIYLMEFCGECRSCKLGFTNQCLNKRG
                     DVGFNRDGGYGPYVLINENVFFPTGDDVPLAEATMLLDVMGTTGHAIRRGLRLWDDVS
                     SLAIAGGGPIGLGVLAMAKILLGPEVHVVISDLSPYRLGLAKRLGGSVIDLGEKSLAG
                     GLRDHGLDEVDLAIDTSGKGVARRSHLGVLSKRGVLVCVGHGEGLTLDVSRDLIEPER
                     AVLGSEYFCYGELASNLEILREHSSYLSQIITHRFPLQEIKEAFETFFRGETGKVLVE
                     Q"
     gene            9354..10343
                     /locus_tag="VHM69_19755"
     CDS             9354..10343
                     /locus_tag="VHM69_19755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2742685.1"
                     /translation="MNGARRVRVALIGAGDWGLQHARVFAGRRDVDLCAIVGRTPEKT
                     RARAKEFGASYYLDLGEMLDRERPDLVSLCLPNLSHFEPTLQVIEAGYPLLVEKPLVF
                     GMGEADRLLSEASRRGLFFAINFNHRYARPVLMAQEDIEQGLLGDVVFATWRFGGEPS
                     DTPHPHANLIETQCHGLDMLEHLCGRITSVMAQMTEKTGHGYSTLVLALSFADGAVGS
                     LVGSYDSSYAYGQTHTLEVNGTRGRVLVEDTVRRYTFQAAGDETARVWQAGYFNDRDR
                     EFHRTFDRHVDEVLGAFRRGDEPPVHARAGRRALELAYATIQSFETGERVATA"
     gene            complement(10356..11792)
                     /gene="menE"
                     /locus_tag="VHM69_19760"
     CDS             complement(10356..11792)
                     /gene="menE"
                     /locus_tag="VHM69_19760"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX2742686.1"
                     /translation="MSEHKLLAPCPLRAAALAAPKAVAIVGARGAISYEELDRLVSEA
                     ALRLGELEAGSRVSLYLPKDERYVVLLLALIRAGHVACPVSDRLPPQGVVPLLKRAAC
                     SAIVSDDEKLLKTVDGALLTLRPETLLRESSQTSSDRAESTSITLNRPATIIFTSGTT
                     GLPKAALHTFGNHYYNALGSNANIPLTPGDRWLHSLPLYHVGGISILFRCLLAGATVA
                     LPESGTPPGEAMVGLGATHVSLVTTQLLRLLREDVDLLGGLKAVLLGGGPIPAYLVDE
                     AVARGLPIHTSYGLTEMASQVTTTPPGASPEMLHTAGRVLPNREISISEAGEILVRGE
                     TLFAGYVEGESVDHPLDASGWFHTGDLGELDQEGHLRVKGRRDNLFISGGENVQPEEI
                     EEALSRLEGIEEAVVVPVPDEEFGARPFAFVRTNGVALEQLAQTLEQVLPRFKIPISF
                     HPWPEGAQGMKPDRAALGERARRLQSEG"
     gene            complement(11797..13410)
                     /locus_tag="VHM69_19765"
     CDS             complement(11797..13410)
                     /locus_tag="VHM69_19765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2742687.1"
                     /translation="MLAQLTNVSLSFPDKKVLDDVSLTVYPGDRISLVGENGAGKTSL
                     FRMLKGRMVPDSGEVSLAREVRIGYLEQDFAGMVDDPKRTCMEAALEPFWYLIELESR
                     IERLASELGEAGDRASELLAELGEAQQRFEVAGGYEFRARTESTLTGLGLTEAFWDRR
                     VSELSAGQRVRIALARLLLEDHDLILFDEPTNHLDIPAREWLEGHLAGMDAAYVVASH
                     DRRFLDAVSRKVAHLDRGGLTLYPGDYTAFHQQLAQAEEEGWRRYEKSRKLVKKLRRQ
                     AQDYQRWSDAGEKKKRGAADKGFVSHKAAKLMKRSLVAQRRMEEAVESASTEKPFQKD
                     AVRIEFASSRGRGLVHAEDLIVGYAEGRPLTEEISFDLSAGDRLAVLGPNGCGKTALL
                     RTVLGEIPALRGDVRLVPSVKVGYFDQDNRLVPSHLTALEAVLGTERDETLARTVMGR
                     MGVRRETVNKPVAKLSSGERAKVLLAGIILSDNNLLVLDEPTNYLDIETQDVLLEALK
                     DFPGGIMFVSHDRYFVEELATETMTLGIT"
     gene            complement(13531..13755)
                     /locus_tag="VHM69_19770"
     CDS             complement(13531..13755)
                     /locus_tag="VHM69_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742688.1"
                     /translation="MRELVGMEPQELEEQRYARAVVLKPTLVGGILRSLWLAQSTLRL
                     GMMPVVSSAYESGVRTAALVALAAGSTFES"
     gene            complement(13947..14798)
                     /gene="menB"
                     /locus_tag="VHM69_19775"
     CDS             complement(13947..14798)
                     /gene="menB"
                     /locus_tag="VHM69_19775"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX2742689.1"
                     /translation="MTDITWESAGEFEDIKYEKAEGIAKITINRPEVRNAFRPLTVIE
                     MQKALDDAREDHEIGVIILSGEGTEAFSSGGDQKVRGDAGYLDDPDAPRRTPGGESVG
                     RFHVTDLHVQIRRCPKPVVAMVAGYAVGGGHVLHVVCDLTIAADNAKFGQVGPKVGSF
                     DGGFGASVLTQLVGPKKAKEIWFLCRMYSAEEALEMGLVNTVVPLAELEEETVRWCRE
                     MLERSPFALRLLKASFHAHEDGFAGIQQLAHDANLLFYGSEEAQEGRDAYKEKRKPDF
                     SKFPHRP"
     gene            complement(15131..15331)
                     /locus_tag="VHM69_19780"
     CDS             complement(15131..15331)
                     /locus_tag="VHM69_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742690.1"
                     /translation="MSKVERLENEVRTLSTEELATFREWFREFDADAWDREIETDASS
                     GRLDDLADRALKAHRTGKTTSL"
     gene            15454..15915
                     /locus_tag="VHM69_19785"
     CDS             15454..15915
                     /locus_tag="VHM69_19785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2742691.1"
                     /translation="MREARDFMRYAYGRPVYLPDVAAHANLSPYHFLRVYKQAYGETP
                     HEFLTRLRIERAKSLLARGSHNVTEACFEVGFSSLGSFSALFAHRVGLSPSEYRGYAR
                     SSVSVPDTVRPLFVPSCFLAMLYGAADNRNFREAEKPSPIVSSGQSSETGR"
     gene            15919..16335
                     /locus_tag="VHM69_19790"
     CDS             15919..16335
                     /locus_tag="VHM69_19790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2742692.1"
                     /translation="MIQRQSHSTIYVIDQDEALAFYRDMLGFEVNTDMTMDDSGFRWL
                     TLSPKGQPDLEVILAKVSSSPMFDADTAEEMKALVARGAFGIGVFETDDIHGDYERLS
                     KQGVEFVSPPKEEFYGTEAVVKVNSGTWFSLTQPKR"
     gene            complement(16336..>16554)
                     /locus_tag="VHM69_19795"
     CDS             complement(16336..>16554)
                     /locus_tag="VHM69_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742693.1"
                     /translation="SQRSLWGELERLDVPTLAVAGELDGKYVRVSSRMTSLSPWIESA
                     VVPEVGHSVHAEIPAEYISLLERFMAPL"
BASE COUNT         3106 a         5234 c         5132 g         3082 t
ORIGIN      
        1 cgagccgttg gacgcggatc ggtgcaccgt cagaacccca gtacagcaac gactctccag
       61 tgcgcgggtc gtcgaaaggc atagggtcta tgttctcgaa gccgtcgccg caccgcaagg
      121 gctcgtcttc ggggacgaac ggtccgccgg gagaacctga cgtggcgacc ccgaggcaca
      181 tgctctcgcg ctcgtcgtgg acgcccgaga agtacatgta gtatcgaccg tcagcctcga
      241 tgacacccgg agcccagaag tcacgggtcg tgcttgccca ctcgggcttc tccggcaagg
      301 cgtcgggaag taactcccag ttcaccaggt ccctggagcg cgctgcctgg atgtacgcca
      361 ggcgctcgat ggtcaggttt tccgtcgagt acgcgtagta ccagccgtcg tcagctttga
      421 gcacggttgg atcagcgaag tcccatcgta tgacagggtt cgtgtacgtc gcgcgatggg
      481 tcattccagg caactcggag cgtatcgagg gcacggcgag taaaacggca gcgagcagga
      541 ggaccatcag cggcaggacg atgcgccacc tagccattct ctacgggcct cagggagctt
      601 gccgtctgga taagatcttc tctcggcagg ccggagagta tcactagcgt ctctcccctc
      661 acgacgtaga ggtacgtctc atccctgacc gttccccacc aggcgttgtc cgccccctcg
      721 acctccgcgg gctcgtcctc gagctggggg tctacgtcca cgtgcgcctg ccttatgaca
      781 agaccatccc cgtactctgc ggcgagcgga cgcgagctgt ctcccgggat gccccgcacc
      841 gtcagctcag gtctctcgcc tcccgccagg gacgctggct cgaaggtatc agtccgggcg
      901 agttcggcgg ccctctgcca cccgttcacg cgctgctcca tcggaagatc cgagtcagca
      961 gggaacgtga catcgggagg cccaagcgcc agcgcgagca ggcacacctc ggccgtgagg
     1021 accaccaaca gggctatgcc aagcgccctc acgcccctgc caggatgagg tgttctatcc
     1081 cttgatgccc acgtttgcga taccgccgat cagtttgtcc tgggcgaaga agtacacgat
     1141 cagcacaggg agcatgacca ggaggttcgc cgccatgacc tcgttccact ccgtgccgac
     1201 gcgatgcacc atcgtcgcga tcgccagggc gaccgtgaac ttctcggggt cgtcgaggta
     1261 gatcaggggc ccgaagaagt cgttccaggt ccacatgaac tggaagaccg cggccacggt
     1321 cagcaccgga atacacagag gcattatgat ccgccagtag atcgtccagt aaccggcccc
     1381 gtcaaccctg gctgcctcgt cgagttcaat ggggatggag cgcatgtact ggcgcatcag
     1441 gaagatgaag aaggcgttgc cggcgaacgc cggcacccag agcgggtagt aggtgccgta
     1501 ccagcctatc cggtagaaga gcaggaactg cggtatgagc agcacgggag caggcagcag
     1561 catcgttgcc accaggaacg tgaagagtac gttcttgcca cggaaccgca gccgcgcgaa
     1621 cgggaaggcg attagcgagg atgagaggac tacgcctatg acgttcatga caacgaggaa
     1681 ggcgctgttc aacccgtagc ggatgaactt ctccttgaag agggcgtcgt ggaaggtctg
     1741 ccagtgccag tactccgtcg ggaaccactc cggcgggaaa gtgaataccg gggccgtatc
     1801 aggcttgagg gcggcggtca gcatccagcc gacgggcaac aggatgaaca ggctggcaag
     1861 gacgagcaac gcatacacga acgccgcgtg cgccagccgc ggaagacgcg ggaaacgcgg
     1921 cgcccggatc tctttccgct cggtgtgctg cgccacgcta ctcctcggac tcgtagaaga
     1981 cgaaatgacg ctcggcccga agcatgatta ggaccatgat cacgccgatg atcgtgagga
     2041 tccaggctag agccgtaccg tatccgatga acccctggtc gaacgccacg tcgtacaggt
     2101 agaccatgta gaaaagtagc gagtcgtccg gcccgcccag accttcgctg cccctcaggg
     2161 tgagcacgaa gagcggtccg aagatgagca gggcgtctat gacagcgttc agcagcaaga
     2221 agaagatcgt cggagagagc atcgggagcg tgatgtgcct gaacttcccc caggcccccg
     2281 cgccgtcaat ggtggccgcc tcgtaaaggt gaggcgggat gttctgcaat ccggcgaggt
     2341 agatgatggc catcccccca accccccaca gactcatcaa cccgaccgcc ggcatcgccc
     2401 actggggatc ggccagccag tacggcgggt tgtccactcc gagcttgatc aggacgtagt
     2461 tcacggcgcc gagctcgggg ttcaagagct gtaaccagag gacggcaacg gccacggccg
     2521 agaccacagc gggtatgtac aggatggtcc tgaacacgtt catcccgaag tacttctggt
     2581 tgagcaggag cgagagcaac aacccggcag ccaggaagag cggcgtcgtc aggaagatcc
     2641 acttcagcgt tatgtacagc gagtggtaga agaaaccgtc ttgcgtgaac atgtagacgt
     2701 agttcgagag cccgacgaag tgctccctcc cagcgctgat gagacgcttg tccgtcatgc
     2761 tgatgtacag cgcgtatgcg agggggatcg cgaggaagaa gagcagccct gcgaaccacg
     2821 ggagcaggaa tagataggcg gtccgctcct cggccctggc cggcgcgctc ctgcgcggtc
     2881 gcgacacctg ctgcgtgccc agctgttgcg tcggcgcgct catggccgcg caccaccggg
     2941 cccgctaccg tagggagtac ttctcaagca agcgcctccc agtccttcca ggccttgtcc
     3001 agaatgggct gcgtcttctt cgccgcggcg tcgaggatcg gctcggcatc ggtcttaccc
     3061 tggacggtca tctcgttata cacctctacc gaggcatacg cgccctcggg catcggaggg
     3121 acgaacggct gagcctgcgc cgccacctcg agcacactca ggtattcctc cttgatcggg
     3181 tcccccttcg accagtcgat ctccctggcg acactgagcg agagcggcgg atcggacgag
     3241 acctcggccc tgatgcgctg cgcttcggtc cccatgaact gcaagaacgt gagtgcagcg
     3301 tgcaggtgct cggactcgac gaacgttccc cacggcgcgg tccaggtgtc tacgaaagac
     3361 tccgagccgc ttatgacagg ccacggcgcg atgccgaaat tgatgttcgc cgcctcgtag
     3421 tcctgcatga acgtgaagtc cgtccacgtc atcgcgatgc gcttctgcgt gaaaaggtcg
     3481 ggctcggcca ccgtcgaggt cacctgactg ctcggcgcga gcttgtccct gacgagcgcc
     3541 gtccccaggt tccacgcctc gatctcgggc tcggtgttga gattgccgag gatcttgcgc
     3601 ccatcggggc cgaagaccca cctctgccag atgccgaacc cccactcggg cgccgaagcg
     3661 ccatataccc tcttggtcgg gtcggagttc ggctgcgcca ccgcgcgcgc caggtcggcg
     3721 tacccaggcc actctagcga cctgtccgtc ggcgggtact ccgcgccagc ctcgtcgaag
     3781 aggtccttgt tgtacacgat gatgttgcca cccaggaagt cgccaacacc gaagacaccc
     3841 tgctccggcc caccctccag cgtgaaacgc gcaacgccgc cgggcgcgaa gtcatcgacg
     3901 gagatacccc aggccttgta gaacggggcc aggtcggcgg tcagatgggc cttcatccat
     3961 gccctctcct ccatcaccgc gacatcgggt gggttgtggg cctggagcgc gacgttgacc
     4021 ttggttacgt agttctcctc cggataggtt acgtacttga gcttgatgtt cgagtactct
     4081 ttctcgaacg ctttcttata cgccttcatg gaggcagggt cctgggcgta catccatatg
     4141 gacaactcca catccggacc gtcgtaaggt ttccagttct cagccagctt cgtcagctcg
     4201 gagttctctc cggcctgtat cttgccgata tcgacctcgc cgccacctcc tcctttcttc
     4261 gccccaggag gcccgccgcc ttcatccaaa ttgcacccgg ccagcatcgc gccgccggcc
     4321 atcatcgcgg agaggcgcaa gaagtgtcca cggctgatcc tgtgttgccc ctgcatccct
     4381 atctcctcct gacaccccga atactcgccg tagcaccccc gccattctcc tcgcgacagc
     4441 gcacgacccg gctagcggta tccgacaatc ctacttatac tatttgcagc cccacttgtg
     4501 ccacctataa tcccacttat gacatagtat aaagtatctt atcctggcgc aacaggaggt
     4561 tgcatcggtg tgctggtggc gggggtgacc atggcggatg ggacagggag gtttggatgc
     4621 cgcgcgaact gatagctctg gagccgcgga tgccggtttt gcgggagtac gaggagccgg
     4681 agttgcgcct gcggcaggtc cgcattcgca cggagttcgc ctcgcccaag cacgggacgg
     4741 agatggtagg tttccggaac gagccgtcgg cgcgacgtcc ttacgacgct gaatggggat
     4801 gtgtgatacc gcgggaagcg ggagagggtc cgcgcaactt ccccttgggc aacatggcag
     4861 tcggtgtcgt taccgaggtc ggacccgaag cgacgcgtct ccgggttggg gatcgggtgt
     4921 tcgggcatct gtccatccgt gagacgcaca ccgttgacga ggattatgtt gacccgttgc
     4981 ctgacgcgct cgaagcggaa tcggcggtgt gcctggatcc cgcggtcatg gcgctggcga
     5041 tgagggacgc ggagatccgt ctcggggacc aggtagccgt tttcggactc ggcgcgatcg
     5101 gcctgatggc cgtgcagatg gcgcgtatcg ccggggccga catggtgata ggcgttgacc
     5161 ccatagaggc caggcgggaa gttgctgagg cattaggcgc ggacgtgacg ctcgacccgc
     5221 gcgagaacga cggcgatgct ggaatgagca tccgacgcat ctccggcgcc acgctgggag
     5281 aggtcgagcg ccctgagggg aagcgtgttc tgggcggtta ttgggaggtg ccgacgcagt
     5341 acggtcagcg cggcgttgac gttgccatcg aggcttcggg gagcgtcagg gcgctgcagc
     5401 aggccatccg ggccacgcgg ttcggaggta cgatctgcat gatctcgttc tacggcggcg
     5461 acgccgccgg gctgaggctc ggcgatgagt tccacgtcaa ccgccagcag ctcgtctcgg
     5521 cccgcgtgga aagcctgccg ctgcgcgacg ctcctgcatg gacgcttgag cggcttgtag
     5581 aagtcgggtt gcgctggctg gcgtcaggcc gcttacgcac ggaaggtatc gtgacccccg
     5641 tggtgccttt cgaggagtcc gtcgaggcat accgcgagat cgacgagcac cccgagcgtt
     5701 cgatcaagct cggtatcagc ttcgattagt aggactgtcg gggacgttat gatactcggg
     5761 ctcaagcgag gatcggtgga ggtcgtgccg catgaaggtc agtggcacga tgtcttcgag
     5821 cgagaacgaa acagcatcct cgaccactcc gccggtctcg tgatcgacgt acagcacgtt
     5881 ggcagtaccg ccgttcccgg cctggatgcg aagcccatca tcgacatagc tgttggcgtg
     5941 gacaccccac gcttggttgc agttctctcg agaatcctga ccggcatagg ttggatctat
     6001 cgtggagaca agggccagga cggcggccac ctcttcgtca aggaggcggc gcccgacttt
     6061 cgcacacatc atctccacgt ggtcgtgatc gatgacggtc gatggaagga ctaccttgcc
     6121 tttcgtgaca tcctgaggcg gaatgaacga gtccgtaatg aatacgccgc cctcaaacgc
     6181 gagctggaga agatgtacag ggatgaccgg ggttcgtata cctcgcgcaa gtcagagttc
     6241 gtgagtgcgg tccttgcaaa gcatgcggga aagagagagg cctgacccgg tacttccgcc
     6301 gctcgactcc agggtgtccg tgagcccgcg ctattattac aaatgtacaa ccgcctctgg
     6361 cgtatctagt cgaacagtgg gatcacctcg cgggcgaagc ggcggaccgc atcatggtcg
     6421 taggcttcgc gcggaatcgt gacgatgaaa tagtctatcc ctgcgtcgat cacgggctgc
     6481 aggcgctccc tgaccatctc aggcgtgccg acgatggtgc ccgtcgcgta atcgtcgaag
     6541 gagcgggagc cacgcgcggc ggccgtcgcc tgctccgccc cggccatgtc gtcgacgacg
     6601 tggatgtcga cgctggtgga cttgatgatc tcgtcgtaat cgcggcccag gtcgtcgcag
     6661 tgacgcttca ggacatccag cttgtggctg atcgtctcga tatcggcggg gaccgagcct
     6721 gcgacgttgc aggcgtcgcc ccactgtgcc accagtttta gcgtcaccct ctcaccgcca
     6781 cccccgatcc agaacgaagg atgaggcttc tgcacgccct tgggttcgtt gatgggcccg
     6841 tcgatcgtgt agtacttgcc ctcgaacttc gggtagtcct ccgtccacat gcggtggatg
     6901 atctcgcacg cctctcggaa catgcccatc ctgtcgcgca gctcggggaa gccgtagccg
     6961 tatgcgcgcc actcgtgctc gtaccagccc gcaccgatgc cggcgtagag cctgccatgg
     7021 ctggcgacgt ctacggttga ggcgatcttg gcgtacagcg ccggattccg gtagccgttg
     7081 cagccgacca tctgcccgat cttgacgttc tgcgtatccc gcgccagcgt cgcgcttata
     7141 gtccatgcct cgaagcaggt ttcgagctcc ggggttggca cggtatggaa gtggtcgaag
     7201 acccagatag agtcccaccc accctcgtcc gccgcgcgag cgacgctcgt catggtctcg
     7261 tattgttcga tggggtcttc tatctcggcg agatccatcc gccagccctg cggcacgaag
     7321 accccgaaac gggtcgtcat cgtctgctcc tttcggttta ctaagccgcg tcccacaact
     7381 cccgcaacag cccggcgcac tccacgagag attcctcata aggaccggcg accccgcact
     7441 cgataccgag atatccgtcg taaccagcct ctttgagcgc ggcgaagccg ggcctgaagt
     7501 cgaggtgtcc cctgccaggc tggaggcgct ggctgtcggc aacgtggacg tgaactatgt
     7561 gtggtcctgc cgccctgatg ctcgcggcga tgtcagcctc ctcgatgttc atgtggaaga
     7621 agtcggccat tatcttgacc ccttccacct gctcggcgaa ggcaacgccc tgctccaggc
     7681 ggttcacgag gtgctgctcg tagcggttga gcggttcgag gaagatcgga actccagcgt
     7741 cgcggacggc gggcgcgagc tcctcgagct gctcgacgaa aagctcccgc tccagatcgg
     7801 cagccgtctt gtacggggag aggtccggca gacgcggcgc cgagccaaac tggggaactg
     7861 tcaggacacc gaccgcgccg agcgccgccg ccaggtggag ccgccgcctc atcaagtcct
     7921 tcgccgcgga ccgttcagca gggtcagggt cgatcagggc ggtgctcccc gcgacgttcg
     7981 ccgcccgcac tggcgagccg gcgaaggtct cctccagctc ccgttgcggc aggtcgagcg
     8041 aggccgccgc cagctcgatg ccgtcgaacc cgatgccctc gagccacgct agcttctctg
     8101 ccaacgtccc gccccggctc atctcctcac gcagcgcaag cttcatgaaa tatcctctct
     8161 atccctgatt cccccgaagg acgggcctga ttgtatgagg gagcctcact ccacgcaagg
     8221 accccttccg agggtttgcc tgcctgggcg aagtcgcgtg tggcttttgc tagactcgat
     8281 gaccgatgtg aggaggagag ccttaccgca tacaggtcat ctcaattgag cggctccgta
     8341 ccagcagaga tgctcgtccc cagattcctg ggcggaggag aaatcgggtt cgcgccgaaa
     8401 tccgttccgg aaccagggtc gggacaactg ctgatccagg tcaaagcgaa cgcgctgtgc
     8461 gggtccgaac ggtggcagta cttcgacgga tccggggtga cgccgggcca cgaggccgcc
     8521 ggaatcgtgg tgaagggagg accgcagaca cgcaccccgc ttgacactcc aggtgtgatc
     8581 tacctgatgg aattctgtgg cgaatgccgt agttgcaaac ttggattcac caaccagtgc
     8641 ctgaacaagc ggggcgacgt ggggttcaac cgggacggtg gttacgggcc ctacgtcctg
     8701 ataaacgaga acgtcttctt ccccaccgga gatgacgttc ccctggcgga ggcgaccatg
     8761 ctcctcgacg tcatgggcac tactgggcat gcgatcagac gcggactgcg cctgtgggat
     8821 gacgtgtcgt cgcttgctat cgccggtggc ggccccatcg ggctcggtgt gctagccatg
     8881 gcgaagatcc tgcttggtcc tgaggtacac gtcgtgatct ccgacctatc accgtacagg
     8941 ctcggcctcg caaagcgtct cggcggcagc gtcatagatc ttggagagaa gtcgctcgca
     9001 ggaggcctca gagaccacgg cctcgacgag gtggatctgg cgatcgacac gagcgggaag
     9061 ggtgtggcca ggcgatctca cctgggagtc ctctccaaac gcggggtact ggtgtgcgtg
     9121 ggacacgggg agggcctgac cctcgacgtc tcgcgggacc tgatcgaacc ggaacgggct
     9181 gtgctcggaa gcgagtactt ctgctacggc gagctggcct cgaacctcga gatactgcgt
     9241 gagcactcgt cgtacctctc ccagatcatc acgcacaggt tcccccttca ggagataaag
     9301 gaagccttcg agaccttctt ccgtggggag acgggcaagg tgctggtgga gcaatgaacg
     9361 gagcacggcg agtcagggtc gccctgatcg gggccggaga ttgggggctg caacacgccc
     9421 gtgtcttcgc aggacgccgg gacgtagacc tctgtgcaat cgttggaagg actcccgaga
     9481 agaccagggc gcgggcaaaa gagttcggcg ccagctatta cctcgatcta ggggagatgc
     9541 tggacagaga gcggcctgat ctcgtaagcc tctgcctgcc caacctgagt cacttcgagc
     9601 cgaccctcca ggtcatagag gcgggatacc ccctgcttgt cgaaaaaccc ctggtcttcg
     9661 gtatggggga ggcggacagg ctcttgagcg aggcgtccag acgcggtctg ttcttcgcga
     9721 tcaacttcaa ccaccgctac gcgcggcctg ttctgatggc gcaagaagac atcgagcagg
     9781 gcctgctcgg cgacgtggtc ttcgccactt ggcgtttcgg cggggaaccg tccgataccc
     9841 cccaccccca cgccaacctc atagagaccc agtgccacgg cctcgacatg ctcgaacatc
     9901 tgtgtggccg catcacatcc gtcatggccc agatgaccga gaagacgggc cacggttaca
     9961 gcacgctcgt cctcgccctg agcttcgccg atggggctgt cggcagcctg gtcggctcat
    10021 acgactcttc ctacgcctac ggccagactc acaccctgga ggtcaacggc acgagggggc
    10081 gcgtgctcgt ggaagacacc gttcgcaggt acacgttcca ggctgcgggc gacgagacgg
    10141 ctagggtctg gcaggcagga tatttcaacg acagggaccg cgagttccac aggactttcg
    10201 acaggcatgt agacgaagtg ctcggcgcct tcagacgcgg cgatgagccg cctgtacacg
    10261 cccgcgcagg ccgccgggca ctcgaactcg cttacgcgac tatccaatcg ttcgagaccg
    10321 gagagcgtgt ggcaacggcc tgatcgcgca gcatctcatc cttcgctctg caaccgccga
    10381 gctcgctcac ccagggcggc ccgatcaggc ttcatgccct gtgctccctc gggccacggg
    10441 tggaaggaga tcgggatctt gaaccgcggc aacacctgct ccagggtctg cgccagttgc
    10501 tccagagcca cgccgttcgt tctcacgaag gcaaatggcc tggccccgaa ctcctcgtca
    10561 ggtacgggca ccaccacagc ctcctcgatc ccctccaatc tagagagcgc ctcctctatc
    10621 tcctcgggct gtacgttctc gccacccgat ataaagaggt tgtccctgcg ccccttgacc
    10681 cgcaggtggc cttcctggtc cagctcgccg aggtctcccg tatggaacca accgctagcg
    10741 tcgagaggat ggtctacgct ttcgccctcg acgtagcctg cgaagagcgt ctcgcccctg
    10801 accaggatct ctccggcctc cgagatcgag atctcgcggt tgggcagaac acggcccgca
    10861 gtatgcaaca tctccggcga agcacctggc ggcgtcgttg tgacctggga ggccatctcc
    10921 gtgagaccat agctggtgtg gatcggtagc ccgcgggcca cggcctcgtc aacgaggtat
    10981 gcagggatcg gacctccgcc cagcaataca gccttgagtc cacccagcag atctacgtcc
    11041 tcccgaagca gcctcagaag ctgagtcgta acgagcgaga cgtgtgtagc gccaagcccg
    11101 accatggcct ctccgggcgg cgttcctgac tcagggagcg ccaccgtcgc cccggcgagc
    11161 aggcaccgga acaggatcga aatccccccg acgtgataca gcggcaacga gtgaagccac
    11221 ctgtcgccag gtgtaagcgg gatgttggcg ttcgatccga gggcgttgta gtagtggttt
    11281 ccgaaggtgt gcagcgccgc cttcgggagg ccggtggtgc ccgaggtaaa gatgattgtc
    11341 gcgggacggt tcaatgtgat tgatgtcgac tccgcccggt cagaggaggt ctgggaactc
    11401 tctcgtagca gtgtctccgg tcgtagtgtg aggagggccc catcgaccgt tttcaagagc
    11461 ttttcgtcgt cggagacgat ggcggagcag gcggccctct ttagaagcgg cacgacgccc
    11521 tgtggtggca gacggtcgct gacggggcag gctacgtgtc cggcacggat cagggcgagc
    11581 agcaacacga cgtaacgttc gtctttggga aggtagagtg atacgcggct ccccgcttcg
    11641 agctccccga gacgaagagc tgcctccgag accagccggt ccagttcctc gtaagagatg
    11701 gcgccccgcg cgccgacgat ggccacggct ttcggcgcgg cgagggctgc agcccgcaag
    11761 ggacagggcg caagtagctt gtgttcactc acgtccctat gttatcccga gcgtcatggt
    11821 ttcggtcgcg agctcttcca cgaaatagcg gtcgtgggag acgaacatta tcccgccggg
    11881 gaagtccttg agtgcctcca gcagtacgtc ctgggtctct atgtcgaggt agttcgttgg
    11941 ctcgtcgagg acgaggaggt tgttgtccga gaggatgatc ccggccagga gcaccttggc
    12001 gcgctcgccg gaactcagct tcgccacggg cttgttgacg gtctccctcc tcacacccat
    12061 cctgcccatg acggttcggg ccagggtctc gtcgcgctct gtcccgagga ccgcttcgag
    12121 ggccgtgagg tgtgaaggca cgagcctgtt gtcctggtcg aagtagccga ccttcacgga
    12181 agggacgaga cgcacgtcgc cgcgaagtgc cggtatctca ccgagcacgg tacgcagcag
    12241 agcggtcttg ccgcaaccgt tcggaccgag gacagcgagc ctgtcccccg ccgatagatc
    12301 gaacgaaatc tcctccgtca aaggacgccc ctccgcatag ccgacaatca ggtcctcggc
    12361 gtggaccaga ccacgtcctc tcgaagaggc gaactctatc ctgaccgcgt ctttctggaa
    12421 cggcttctcc gtactggcgc tctcgacggc ctcctccata cggcgctggg cgacgagcga
    12481 acgcttcatg agcttcgcgg ccttgtggct cacgaacccc ttgtccgccg cgccccgctt
    12541 cttcttctcg ccggcgtctg accacctctg gtagtcctgt gcctgcctcc gcagcttctt
    12601 caccagcttc cggctcttct cgtacctgcg ccatccctcc tcctcggcct gagcaagttg
    12661 ttggtggaac gcggtgtagt cgcccgggta gagggtgagc ccgccacggt cgaggtgggc
    12721 caccttcctt gagacggcgt cgaggaagcg cctgtcgtgg gaggcaacga cgtatgctgc
    12781 gtccatcccc gcgaggtgcc cctccagcca ctcgcgggcc gggatgtcca ggtggttcgt
    12841 cggctcgtcg aacaggatga ggtcgtgatc ttccaacaac agcctcgcga gcgcgatccg
    12901 caccctctgg cccgcggaga gctcggacac cctgcggtcc cagaacgcct cagtgaggcc
    12961 gagcccggtg agagtggact ctgtccttgc cctgaactcg taaccgcccg cgacctcgaa
    13021 gcgctgctgc gcctcgccca gttcggccag gagctccgaa gcccggtcgc ccgcctcgcc
    13081 gagctccgag gccagacgct cgatgcggct ttccagctcg atcaggtacc agaaaggctc
    13141 aagcgccgcc tccatgcagg tgcgtttcgg atcgtccacc atgcccgcga agtcctgctc
    13201 cagatacccg atacggactt ccctggcgag agatacctcg cctgaatcgg gcaccatgcg
    13261 tcccttcagc atgcggaaca ggctcgtctt tccagctccg ttctcgccga cgagcgagat
    13321 cctatccccc ggatagacgg tgagggagac atcgtcgagc accttcttgt ctgggaacga
    13381 gagcgagacg tttgtgagtt gtgcgagcac gtaaaggtcc tttgctggac gaggggaaag
    13441 acttgttgca aaggaagctc gctacatgcc ggatagtata tcctaacgcc cccgagcccg
    13501 cacggagcca tcggagaagc tagagcgtct ctagctttcg aaagtcgacc ctgcggcaag
    13561 cgccaccagg gcagcagtcc ggacgccgct ctcatacgcc gaactgacga cgggcatcat
    13621 gccgaggcgg agtgtgcttt gcgccaacca caagctacgc aagatgccgc cgaccagtgt
    13681 cggcttgagc accacggccc gcgcgtaacg ctgctcctcc aattcctgag gctccatccc
    13741 gaccagctcg cgcaaccgcg ccgcggcttc ttccagactc tccccgctga agccggcgca
    13801 ctctcgcccc agccttccga accgtcatcg ccggtcagcc tcagcaggag gccttcgcgg
    13861 taatggagcg tcagcggttg cgagaagggc agtctgtaac ggtagaggtc gaagcgtctc
    13921 agaagcacgc cggcttcgcc gccggcctaa gggcggtggg ggaacttcga gaagtccggt
    13981 ttgcgcttct ccttataggc gtcgcggccc tcctgtgcct cctcgctacc gtagaagagg
    14041 aggttcgcgt cgtgggcgag ttgttggatg ccggcgaagc cgtcttcgtg ggcgtggaaa
    14101 ctagccttca ggagacggag tgcgaaaggt gagcgctcca gcatctcgcg gcaccagcgg
    14161 accgtctcct cttccagttc ggcgagtggc acgaccgtgt tgacgagtcc catctcgagc
    14221 gcctcttcgg ccgagtacat acggcagagg aaccagatct ccttcgcctt cttcggaccc
    14281 acgagttggg tgaggaccga agcgccgaaa ccgccgtcga agctgccgac cttcgggccg
    14341 acctgcccga acttcgcgtt atccgcggcg atcgtgaggt cgcatactac gtgcaagacg
    14401 tggccgccgc cgacggcgta tccggcgacc atcgctacca ccggcttcgg gcaacgccgg
    14461 atctggacgt gcaggtccgt gacgtggaag cgtccgacgg actcaccgcc cggggtgcgg
    14521 cgcggcgcgt ccgggtcgtc gaggtatccg gcgtcacctc gcaccttctg gtctccgccg
    14581 gaggagaatg cctccgtacc ctccccgctc aggatgatga ccccgatttc gtggtcttcg
    14641 cgggcgtcat ccagcgcttt ctgcatctcg atcaccgtca gcggacggaa ggcgttgcgc
    14701 acctcggggc ggtttatggt gatcttcgcg atcccctccg ccttctcgta cttgatgtcc
    14761 tcgaactctc cggctgattc ccaggttatg tcagtcatga gagagattat agaagatcgg
    14821 aatactaatg gatcgtccca gacggcttta tcgaaattgc tactgcatcg agcgcaaggt
    14881 tagcccaaga tcttgtcgta atctgcatgg cttccgatcc agaaccagag tacgccgtct
    14941 tctacttcga ccccgagcgc acgatgcgac aggccgacac gcgccgaaaa gaatcggcct
    15001 accttcttaa aacgcagaga cggatgttga ggatcgttct ttagcagatc gaagttcttg
    15061 tccgcgacct tgcgaagcgg atctggcagt ttaccgtaac tctcccagaa ctccggggag
    15121 gcgaagggcc tcaaagagag gtggtcttac cggtgcggtg agctttcaaa gctctatccg
    15181 caaggtcatc gagccttccg gatgatgcgt cagtctcgat ctcacggtcc caggcgtcag
    15241 cgtcgaactc gcggaaccac tcgcgaaagg tagcgagctc ctcggtggag agagtgcgaa
    15301 cctcgttttc gagcctttcg accttgctca ctcagacctc cttcggacga gcaacgaaga
    15361 ttatagcagc gggaatacct tgccaataat gccattggct ctcggaccac gtcctgtact
    15421 ctttctacga tgcaccctca gacacacaga gacttgagag aagctcgcga cttcatgcgc
    15481 tacgcttacg gtcgtcccgt ctatttgcct gacgtcgcgg cccatgcaaa cctctcgccc
    15541 taccacttcc tgcgcgtcta caagcaggcc tacggggaga cgccgcacga gttcctgacc
    15601 agactgcgaa tagagagagc gaagtcgttg ttggcgagag ggagtcacaa cgtcaccgaa
    15661 gcgtgcttcg aggtgggctt ctcaagcctg gggagcttca gtgccctgtt cgcccatcgc
    15721 gttggtctgt ccccctccga gtacaggggc tacgctcgat ctagtgtctc cgttcctgat
    15781 acggtcagac cgctcttcgt tccatcgtgt tttctggcca tgttgtacgg ggccgcggat
    15841 aaccgcaatt ttcgagaagc cgaaaagccg tctccgatag tatcttcggg ccaaagcagc
    15901 gagaccggga ggtaagcaat gatccaacgc cagagccact cgacgatcta cgtgatcgac
    15961 caggatgagg ccctcgcttt ctaccgtgac atgctgggtt tcgaggtcaa tacggacatg
    16021 accatggacg actcgggctt tcgctggctg acgttgagcc ccaaaggaca gcccgacctc
    16081 gaagtgatac tggccaaggt atcttcgagc ccgatgttcg acgccgacac agccgaagag
    16141 atgaaagcgc tagtcgccag gggcgctttc ggaatcggcg tcttcgagac cgacgacata
    16201 cacggcgatt acgagcgcct gagcaagcag ggcgtcgagt tcgtatcgcc gcccaaggaa
    16261 gagttctacg gcaccgaggc cgtcgtgaaa gtcaactcgg ggacctggtt cagcctcacc
    16321 cagcccaaga gatagtcaca agggggccat aaaacgctcc agcagggaga tatattccgc
    16381 ggggatctcg gcgtgcacgc tgtgtcccac ctcagggacc actgcggact cgatccacgg
    16441 gctgaggctc gtcatgcgcg atgagacacg cacgtacttc ccgtccagct ctccggcgac
    16501 ggcgagcgtg gggacatcga gacgctccag ctctccccac aacgagcgct ggct
//
LOCUS       DATAIR010000831         6008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_85060, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000831 DATAIR010000000
VERSION     DATAIR010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85060"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VHM69_19800"
     CDS             <1..1174
                     /locus_tag="VHM69_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742694.1"
                     /translation="DVVLFAGEQRFTYQLALGTVSPGRHTVEVAFNPEKSPAGATGAQ
                     IHNLSTQVVSAKDPASLAYQYSPILYGRDLPEIPGQYENNYTDVPLLMYHTVSKDTTS
                     GNTTIEYTVIWSNEDGGTNTPALMARWGRSTDIEWIYRVTLDPQNNIVSEYYQAPNHA
                     TLPFTGAKEGNHPLLQTSTSNNNLTQVTDPALSSGYRFFMDPSQTLPDNRAREVVMDE
                     NRWSYQIMAKEMVREGKIEANPKPSTPEVSDQRNYLYIEIDKDTTYATPPTSGNWVGT
                     AVAVKLQGDHRWYTSNHDIPDWSIQRDIPAATTVELPPGTTTNDIEAIKVFAVPVDSN
                     PNDATPAPNNYTIHFTDINRAFFLESNYLPGSSFIDWSGDVVLTPQNPEAIAWQAR"
     gene            1385..3451
                     /locus_tag="VHM69_19805"
     CDS             1385..3451
                     /locus_tag="VHM69_19805"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX2742695.1"
                     /translation="MSNRLRSRGPLAAFGAALAFATVLLWAASAQSAPGQEFDLQAHR
                     GGLGLRPESTLSSFGNAMQLGVSTLELDVQITEDGVAVITHDRKITGVKCQDTQPAFP
                     GDPEYPYVGKYIKTLTLAQVKTLDCGTRRPTDPNDPFVKTMEMVPGSKMPTLTQVFDL
                     VKRYGADDVMLNVETKVEAGAPEETAPREQFVQVVAHDVREAGMIDQVTIQSFDWGAL
                     MRMREVEPRLPIVALTNRDFLQCGQPGKSPWLGGIDADKYDCDLVRAADSFGADAISP
                     VHGFPQNGTVSDPNYEPYVTSKMVSDAHAAGMKVIPWTIDDKPTMEKLIDDGVDGIIT
                     DYPDRLREVMAARGFALPQPIASPFDVQAHRGGRMYRPENTLAAFNYALKNPDVSTLE
                     LDTGVTEDGYLVVAHDRAINQRVCKDTAPVKRNDSEYPYVGDLINHLTLAQIKTLDCG
                     SITQPNLPKQQAVPGEKMPTLAEVFKAVRESGRSDIGLNVETKISPIAPHDTVKPEVF
                     ANKLVKEIRAAGMTDRTTIQSFDWRTLRESRKLDPRIKTVALVWQYGPKECQTLADEC
                     SLRAVYNDPSVKSPWTAPQDWWQVRDLGKLVQGVGASTVSSNWQVHDPNQGTVVSDDW
                     YLREDPAYYHGPTVPELQAQGIKVIPYTINDEPTMQRVIDLGVDGIISDDHELLIQVA
                     KRNGLR"
     gene            3642..5450
                     /locus_tag="VHM69_19810"
     CDS             3642..5450
                     /locus_tag="VHM69_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX2742696.1"
                     /translation="MSSRLRSVGVVAMLALASMLAMVLVWAVSAQSATRLKPTPEDRI
                     ANVQLLGVNDFHGNLQSPRTLPYNGTQVPVGGAEYLDAYLDKYEAQNPDRTIRVHAGD
                     MVGGTPLISSYFHDEPTVYAMNNMEFDVGTLGNHEFDEGGEEMLRMIDGGQRDDGLQF
                     KDGPDGKPINTSDPDFPGANFPYIAANTQYKDSGKTVLPPYQIINRDGVDVGFIGVTT
                     LETPSIVVPDAVAPFNYTDISDTVNKYARQLQKQGVQTIVVLAHAGGFYDSNAKRVTG
                     EIIDETAQMSGDVDVVVAGHSHSRLNDCVDDQTGATRNAEPGGCAEDKLVVEAFSYGT
                     AFDAVDMNIDRTTKDVISSSANIVSTYQTDEKGRKLVKPDPDTAALVKDYKERIAPVA
                     NRVVGTAAEDITRTTTPAGESAMGDLIADAQRKYPGRVNPLPPADFAFMNPGGIRDDL
                     YRNETYANEGYGEITYGELFGVQPFDNQVVRMKLTGDQIYRVLEQQFRADGSSRILQV
                     SCLKFSYNSTNPTGQKITSVTLPNGSQLDRSATYTVAANSFIATGGDGFTVFKEGANP
                     QTLGSDLDALEAHIQSLPQPFTAPNPATEQRITKQG"
     gene            complement(5515..>6008)
                     /locus_tag="VHM69_19815"
     CDS             complement(5515..>6008)
                     /locus_tag="VHM69_19815"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2742697.1"
                     /translation="WALVDERGLRSVTMSQIAEKAGIGRATLYKYFPDVEAILLAWHE
                     RHVSSHLEHLAEVRDQAGSARERLEAVLGAYALIEHRRHASGLAALLHRGEHVARAQQ
                     HLTDIVRELLSEAAETGDVRNDIAPEELASYCLHALSAAGSMQSEAAVLRLVQVTLAG
                     LKP"
BASE COUNT         1297 a         2004 c         1775 g          932 t
ORIGIN      
        1 tgacgtcgtg ctcttcgcgg gcgagcagcg gttcacctac cagctcgctc tcggcaccgt
       61 cagtccgggg cggcacacgg tagaggtcgc attcaacccc gagaagtcgc ctgcgggtgc
      121 gacgggcgcg cagatccaca acctctccac acaggtcgtc tcggccaagg atcccgcatc
      181 actcgcgtac cagtattcgc ctatcctcta cgggcgtgac ctccccgaga tccccggcca
      241 gtacgagaac aactacaccg acgtgccgct gctcatgtac cacacggtga gcaaagatac
      301 tacgagcggc aacaccacca tcgagtacac ggtgatctgg tcgaacgagg acggcggcac
      361 gaacaccccg gccctcatgg cccgatgggg ccgctcgacg gatattgagt ggatctaccg
      421 cgtaaccctc gatccgcaga acaacatagt ttccgagtac tatcaggcgc ccaatcacgc
      481 cacccttccg ttcacaggcg cgaaggaggg caaccacccg ctgctccaga cctcgacctc
      541 gaacaacaac ctcacgcagg tcacggaccc cgccctcagt agcggctacc gcttcttcat
      601 ggatccctca cagaccctgc cggacaacag ggccagggag gtcgtgatgg acgagaaccg
      661 ctggagctac cagatcatgg ccaaagagat ggtaagggag ggcaagatcg aagccaaccc
      721 caaaccctcg acgccggagg tttcagacca acgcaactac ctctacatcg agatcgacaa
      781 ggacaccacc tacgcgacgc cgccaacgtc tggaaactgg gtcgggacgg cggtcgccgt
      841 caagctgcag ggcgaccaca gatggtacac ctccaaccac gacatccccg actggtcgat
      901 ccaacgcgac atccccgcag ctaccacggt cgagttgcca ccaggcacca cgacgaatga
      961 catcgaagcg ataaaggtct tcgctgtgcc cgttgactcc aacccgaacg atgccacgcc
     1021 ggcaccgaac aactacacca tccacttcac ggacataaac cgggcgttct tcctggaaag
     1081 caactacctg cccggctcct cgtttatcga ctggagcggt gacgtcgtgc tcaccccgca
     1141 gaaccctgag gcgatcgcct ggcaggctcg atgagctgac gagaagggcg gagaccgcga
     1201 cttcatccgg ctccgccctt cgttactctc gcgcagagag tcgaggcaat ccccctggta
     1261 gcgcctgggc gaacagaggc ttaacataga ttttacgacc ttgaaacact ggctacggga
     1321 tctgttgctt gacttacgag ggaggcagat cggccgcagt cctgtaggga aagggaggac
     1381 ggggatgagt aacaggttac gttcgagggg accacttgcc gcattcggcg cggcgctagc
     1441 gttcgcgact gtcttgctct gggcggcgag cgcgcagtcg gcgcccgggc aagagtttga
     1501 ccttcaggct caccgaggcg ggcttggcct gcggccggag agcacgctaa gctcgttcgg
     1561 caacgccatg cagctcgggg tgagcacgct agagctggac gtccagatca ccgaggacgg
     1621 cgtcgccgtc atcacccacg atcgcaagat caccggggtg aagtgccagg atacacagcc
     1681 agcgttcccc ggcgaccccg agtaccctta cgtcggcaag tacatcaaga cgctcaccct
     1741 cgctcaggtc aagacgctcg actgtggcac acggcgtccc acggacccca acgacccatt
     1801 cgtgaagacc atggagatgg tccccggctc gaagatgccc acgctgaccc aggtcttcga
     1861 cctcgtcaag cgctatggag ccgacgatgt catgctcaac gtcgagacca aggtcgaggc
     1921 cggagcgccc gaggagacgg cgccgcggga gcagttcgtg caggtagtgg cgcatgatgt
     1981 ccgcgaggca ggcatgatcg accaggtgac gatccaatcc ttcgactggg gcgccctgat
     2041 gcgtatgcgc gaggtcgagc cgcgcctgcc catcgtcgcc ctgacaaaca gggacttcct
     2101 gcagtgcggc cagccaggca agtctccctg gctcggaggc atagacgccg acaagtacga
     2161 ctgcgacctg gtgcgcgccg ccgactcctt cggcgccgac gcaatctcgc cagtgcacgg
     2221 ctttccccag aacgggacgg tgagtgatcc gaattatgag ccgtacgtga ccagcaagat
     2281 ggtcagtgat gcacacgcag ccggtatgaa ggtcatcccc tggacgatcg acgacaaacc
     2341 gacgatggag aagctcatag acgacggcgt agacggcata atcaccgatt acccggacag
     2401 gctgcgcgag gtcatggccg cacggggctt cgcgctgccc cagcccattg cttcaccgtt
     2461 cgacgtgcag gcccaccgcg gcgggcggat gtacaggccc gagaacacgc tggccgcctt
     2521 caactacgcg ctgaagaatc ccgacgtctc gacgctggag ctcgacaccg gcgtcacgga
     2581 ggatgggtac ctcgtcgtcg cccacgaccg cgcgatcaac cagcgcgtgt gcaaggacac
     2641 cgcccccgtg aagcgcaatg actccgagta cccgtacgtc ggagatctca tcaaccacct
     2701 gacgctggcg cagataaaga ccctcgactg cggctccatc acccagccga accttcccaa
     2761 gcaacaggct gttcccggcg agaagatgcc gaccctcgcc gaggtcttca aagcggtcag
     2821 ggagagcggg cgtagcgaca tcgggctaaa cgtcgagacc aagatcagcc cgatcgcccc
     2881 tcatgacacc gtcaagcccg aagtcttcgc gaacaagctc gtcaaggaga tcagggccgc
     2941 aggtatgaca gaccggacga cgatacagtc cttcgactgg cgcacgctgc gcgagtctcg
     3001 aaagctcgat ccaaggataa agaccgtcgc cctcgtctgg cagtacggtc ccaaagagtg
     3061 ccagactctc gccgacgagt gctccctgcg cgcggtctac aacgacccga gcgtgaagag
     3121 cccatggacc gctccccagg actggtggca ggtgcgagat ctcggcaagc tggtgcaggg
     3181 agtcggagcc tcgacggtct cctccaactg gcaggtccac gaccccaacc agggcacggt
     3241 cgtctccgat gactggtacc tgcgtgagga ccctgcttac taccacggcc cgacggtgcc
     3301 cgagttgcag gcccagggca tcaaagtcat cccgtacaca atcaacgacg agcctacgat
     3361 gcagcgcgtc atcgacctcg gcgtcgacgg gatcatcagc gacgaccacg agctactgat
     3421 ccaggtcgcc aagcgcaacg gcctgcgata gagcaacgaa agcagtcatt gaaggggagc
     3481 cctgctcgaa gacttcgagc agggctcctg tctcacaggc ccacttttaa cccaaggttt
     3541 accggcgtga aacacccccg gcgggcttta ctggtaactt atgcggggtg ggaggatgac
     3601 cgccgctttg aaaagccgat agtaagagga aggatcttat gatgagtagc aggttgcgat
     3661 cggtgggggt cgtcgccatg ctggcactgg cctcaatgct tgcgatggta ctcgtctggg
     3721 cggtaagtgc ccagtccgcg accaggctaa agccgacgcc tgaggatagg atcgcaaacg
     3781 tccaactcct cggcgtcaac gacttccacg gcaacctcca gtcgccacgg actctgccct
     3841 acaacggcac tcaggtgccc gtcggcggtg ctgagtacct cgacgcctac ctcgacaaat
     3901 acgaggccca gaaccccgac cgcaccatca gggtgcacgc tggggacatg gtcggaggga
     3961 cgcccctcat ctccagctac ttccacgacg agccgaccgt ctacgcaatg aacaatatgg
     4021 agttcgacgt cggcaccctc ggcaaccacg agttcgacga gggtggcgag gagatgctgc
     4081 gcatgatcga cggcggccag cgtgacgacg gcctccagtt caaggacggc cccgacggca
     4141 agcccataaa cacctcggac cccgacttcc ccggcgcaaa cttcccttac atcgcggcga
     4201 acacccagta caaggactcg ggcaagaccg tcctgcctcc ttaccagatc atcaatcgcg
     4261 acggggtgga cgtcggcttc atcggcgtaa ccacgctcga gacgccgagc atcgtcgttc
     4321 ccgacgctgt cgctcccttc aactacacgg atatctccga cacggtcaac aagtacgcca
     4381 ggcagcttca gaagcagggc gtccagacca tcgtcgtgct cgcgcacgca ggcggcttct
     4441 acgacagcaa cgccaagagg gtgacgggcg agatcatcga cgagacggcc cagatgagcg
     4501 gcgacgtcga cgtagtcgtc gcgggacact cgcacagcag gctcaacgac tgcgtcgacg
     4561 accagaccgg ggccacccgc aacgcagaac caggcggctg cgccgaggac aagctagtcg
     4621 tcgaggcctt cagttacggc acggccttcg acgccgtcga catgaacata gaccgcacga
     4681 cgaaggatgt gatctcctcc tccgcaaaca tcgtcagcac gtatcagaca gacgaaaagg
     4741 gcagaaagct ggtcaagccg gatcccgata ccgccgcgct tgtaaaggac tacaaagagc
     4801 gcatagcccc cgtggcgaac agggtcgtcg gcaccgccgc ggaggacatc acacgcacca
     4861 caacgcctgc aggcgagagc gcgatgggtg accttatcgc cgacgcccag cgcaagtatc
     4921 ctggcagagt gaacccgctg cctcccgctg atttcgcctt catgaaccct ggcggcatcc
     4981 gcgacgacct gtatcgtaat gagacctatg ccaacgaggg ctacggtgag atcacctacg
     5041 gcgagctctt cggggtgcaa cccttcgaca accaggtcgt caggatgaag ctgacggggg
     5101 atcagatcta ccgcgtcctc gaacagcagt tcagggcgga cggtagtagc cgtatcctcc
     5161 aggtgagctg cctgaagttc tcctacaact ccactaaccc caccggccag aagatcacga
     5221 gcgtgacgct gccaaacggc agccagctcg accggagcgc cacctacacg gtggctgcca
     5281 acagctttat cgccaccggc ggcgacgggt tcacagtctt caaggagggg gcgaacccgc
     5341 agaccctcgg cagcgacctg gacgccctgg aggcgcatat ccagagcctg ccccagccct
     5401 tcaccgcccc gaaccccgcg acggagcaga ggatcaccaa acaaggatag ccacacacga
     5461 ctttcctttg acccaggggc gcgcctgacg gcgcgcccct ggaacctcag tggctcacgg
     5521 cttcaggcct gcgagtgtaa cctggacgag ccggaggacc gctgcctcgg actgcatgct
     5581 gccagccgct gagagggcgt ggaggcaata gctggcaagc tcctcgggcg cgatgtcatt
     5641 ccgaacgtcg ccggtctccg ccgcctcact cagcaactct ctgacgatgt ccgtgaggtg
     5701 ctgctgcgcc cgggcgacgt gctcgcctcg atgcaggagc gccgcgagcc cgctggcgtg
     5761 gcgcctgtgc tcgatgagcg cgtaggctcc gagcaccgcc tctagccgct cacgagcgct
     5821 gccggcctgg tcccggactt cggcgagatg ttcgaggtgg ctgctgacgt ggcgttcgtg
     5881 ccaggcgagc aggatcgcct cgacgtcagg gaagtacttg tatagcgtcg cacgtccgat
     5941 gcccgccttc tcggcgatct gcgacatcgt caccgagcgc agccccctct cgtccaccag
     6001 cgcccagg
//
LOCUS       DATAIR010000832         5507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_180549, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000832 DATAIR010000000
VERSION     DATAIR010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180549"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..900)
                     /gene="dapA"
                     /locus_tag="VHM69_19820"
     CDS             complement(7..900)
                     /gene="dapA"
                     /locus_tag="VHM69_19820"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX2742698.1"
                     /translation="MFSGAFTALVTPFRNGEVDVEALEGMVEFQIHNGVHGLVPCGTT
                     GETPAMSEEEDRVVISTVVRVANGRVPVIAGSGSNSTDMAIKYTRTAEEAGADGSLQV
                     APYYNKPTQEGLYRHFATIAENTDLPIILYNIPGRTSVTISAETTARLAEIPNIVGTK
                     EATLSMNMAMDVKSLCGDEFAILSGDDPVTLPLMALGGHGVISVASNVAPAAVSDMVN
                     ALLDGDWERGRELHYELLPLFRALFVETNPIPVKTAASILGLCSDEMRLPMIPLSGEN
                     LDTLRRVMEESNHLLPTPEEV"
     gene            complement(893..2122)
                     /locus_tag="VHM69_19825"
     CDS             complement(893..2122)
                     /locus_tag="VHM69_19825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2742699.1"
                     /translation="MGIIVQKYGGSSVATAEHIRAVAERVKKVRESGHGLVVTVSAMG
                     KQTDRLLRLAGEVSRDPSPREIDQLLSTGELQSVALLAMALHDRGVAATSLTGPQAGM
                     KTTGRYGSGVISGIQPDRLRGLLAEGHVAIVAGFQGVNTLGDIMTLGRGGSDTTAVAL
                     AAALGADRCEIFTDVDGIYTADPRIVPGARRIPAILPSEMAEMAWRGAKVMHPRAVEL
                     GALYGIEIHVKSSFVEGPGTIITGGNDLEHLETRETVAGIVHDLDVSRITLTGIKTGA
                     GTMSRVFSPLAEAGISVDVIVESAPKEGASDVAFTVRRGDFHAALELASEVADSLEGR
                     AEGEEELGKVSVVGTGMLNRPGYAARMFATLGEAGIPIRLVSTSEIQVTCVLPAERVE
                     EAVRVLHRDFELEERDV"
     gene            complement(2389..2760)
                     /locus_tag="VHM69_19830"
     CDS             complement(2389..2760)
                     /locus_tag="VHM69_19830"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2742700.1"
                     /translation="MEQEVMFDNQEQLEKIQEGLLPEETVYAVFDLKGSGTGFVGITD
                     KRVMLQDSAFIKKEKAIVSVPYDRIHALAASEDTGMLGGRGVFSGSKLVLSTSAGAYE
                     LEFRGADKAHTAHNMILTRML"
     gene            complement(3037..3936)
                     /locus_tag="VHM69_19835"
     CDS             complement(3037..3936)
                     /locus_tag="VHM69_19835"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2742701.1"
                     /translation="MADDQHRQQDPTQQYPQPDYPEQDQRDQHPGLEQEMQPEPDYGV
                     ETYRGSGKLEGKKAIITGGDSGIGRAVALAFAREGADVVISYLEDEEADAEETAQTVE
                     DSGRKAVKAPGDVADEAQCRALIQKAVDEFGRIDVLVNNAAHQMTVDGIADVSTELLD
                     RTFKTNIYAMFWLVKAALPHMPEGGSIINVASIQAYQPSPPLLPYSSTKGAIVTFTKG
                     LAQEIVQYGLRANTVAPGPVWTPIIPASMPGDTVSSFGGTSPMGRPAQPAELAPAFVF
                     LASQEASYVNGETLGVTGGQPMP"
     gene            4042..>5507
                     /locus_tag="VHM69_19840"
     CDS             4042..>5507
                     /locus_tag="VHM69_19840"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX2742702.1"
                     /translation="MLEGGEVLTGRKQLMGEFGIVFQNEPVDISEEFSKQENHFFIGD
                     RVEEFDPASGSGKICWKSLSLKQRVSYHQITLQLEDYKVWEDLPPDEYEDDRSLPFDL
                     SFVSPRTVRLRLAARPEGCSGEPTLMLAGDPPSDDSWETHDEGSFVAYHGPSGSVIVG
                     RDPLRFEFRDAGGKLLTSSHHLSDTSGVVNAWPTPFSFVRNTATFHRHIAASFGLSPG
                     EKLFGCGESFTRLDKRGQKLVLWTYDAYSAQTPNMYKPVPFFMSDRGYGIFVHTSAPL
                     TLDLGNSHDGAAVIYLGDDQLDLFFFFGSPKEILSEYTTLTGRSPVPPLWTFGLWMGR
                     QTYSSEKEVRDVAKKLREDRIPSDVIHLDKGWSEIPHRCDFEFSHSRFDDPQKMLADL
                     EEEGFRISLWQLPYFNPKNKLHREAIEKDLVVLTASGEPPVDDAIIDLSKPEADRWYR
                     GKLARLLEMGVGIFTADFGEAAPLSGMYASKKGGFHEH"
BASE COUNT          971 a         1759 c         1664 g         1113 t
ORIGIN      
        1 cgcgggtcaa acttcctccg gtgtcgggag gaggtggtta gattcctcca tcacacgacg
       61 caaagtgtcg aggttctcgc cggagagtgg gatcatgggc agccgcatct cgtccgagca
      121 gaggccgagg atcgaggccg cagtcttgac ggggatcggg ttggtctcga caaagagcgc
      181 gcggaacagc ggcaacagct cgtagtggag ctcgcggcca cgctcccagt ctccatcgag
      241 gagggcgttc accatgtcgg agacggctgc gggggcgacg ttggaggcga cggagatcac
      301 accgtgccca ccgagggcca tcagggggag cgtgacaggg tcgtcacccg agagtatggc
      361 gaactcatcg ccgcagaggc tctttacgtc catcgccatg ttcatcgaga gcgtcgcctc
      421 tttcgtcccc acgatgttgg gtatctccgc gaggcgcgcc gtggtctcgg ctgagatggt
      481 aacggacgtc cgtcccggta tgttgtagag gatgatcggt aggtccgtgt tctccgcgat
      541 cgtggcaaag tgcctgtaca gcccctcctg tgtcggcttg ttgtagtagg gggcgacctg
      601 aagcgagccg tctgccccgg cttcttcggc cgtcctggtg tacttgatgg ccatgtccgt
      661 gctgttggag ccagagccag cgatcacggg cacccttccg ttggcgaccc gcaccacggt
      721 acttattacg acgcggtcct cctcctcgct catggcaggg gtctcgcccg ttgtcccgca
      781 ggggacgagg ccgtggacgc cgttgtggat ctggaactcg accattcctt ctaaggcttc
      841 gacgtccacc tccccattcc tgaaaggtgt gacgagggca gtgaacgcgc cgctaaacat
      901 ctctctcctc caactcgaag tccctgtgca aaactctcac cgcttcttct actcgctccg
      961 cgggcaatac gcaagtcacc tgtatctcgg aggtggagac gagccttatg ggaatgcctg
     1021 cctccccgag cgtcgcgaac atccgcgccg catagcccgg gcggttgagc atccccgtcc
     1081 ctacgacgga gaccttccca agctcctcct cgccttcggc tcttccttcc agagagtcag
     1141 ctacctcact ggcgagctcc agagccgcgt ggaagtctcc gcgacggacg gtaaaggcca
     1201 cgtcggaggc gccttccttc ggggcgctct ccacgatgac gtcgacagag atgcccgctt
     1261 ccgctagagg tgagaagacg cggctcatgg tgcctgctcc tgtctttatg cccgtaagtg
     1321 tgatgcgcga gacgtcgagg tcgtggacga tgccagcgac cgtctctcgg gtctcgaggt
     1381 gttccaaatc gttacctccc gtgatgatcg tacctggacc ctccacaaag gagctcttta
     1441 cgtggatctc gatcccgtag agcgccccga gttccacggc ccgcgggtgc atcaccttcg
     1501 cgcctcgcca cgccatctcg gccatctctg agggcaggat cgcgggtatc cgccgcgccc
     1561 cggggacgat gcgcgggtct gccgtgtaga ttccatcgac gtccgtgaag atctcgcatc
     1621 ggtccgcccc gagcgccgcg gcgagcgcca cggccgtcgt gtcagacccg ccgcgtccta
     1681 gcgtcatgat gtccccaagg gtgttgaccc cctgaaatcc cgcgacgatg gcgacatgtc
     1741 cctcggcgag caagccgcgc agccggtcgg gctgtatccc ggatatgacc ccggatccat
     1801 agcggcccgt ggtcttcatg cccgcctgcg ggccggtcag cgaggtggca gcgacgcccc
     1861 tgtcgtgcag cgccatcgca agcagcgcca ccgactgcag ctcgcccgtg gagaggagct
     1921 gatcgatctc acgcggtgaa ggatcgcggc tcacctctcc cgccagccga agcagcctgt
     1981 ccgtctgctt ccccatcgcc gagacggtca cgacgaggcc gtgacccgac tcgcggacct
     2041 tcttcaccct ctccgcgacg gcccttatgt gctccgccgt cgccaccgag ctcccgccgt
     2101 atttctggac gatgattccc aaagacgcct caacgctcct tccaactcgt agcctaacgc
     2161 atcgtcaaat aggtgtcagg tttcaggcat caggcggcgt tgcgccctta caccaatggt
     2221 cccatttaca tacagtcggc gctctgcacc aaaagccttt cgtcgggaat ccgcggaagg
     2281 gcggattgca tcgaatttaa gcatagtagt tagattctag tgcaaggctg cttccacgaa
     2341 aaagccgctc ctagaggagc ggcggatgcc ggtgggtttc tgtactcgct ataacattcg
     2401 cgtcaggatc atgttgtggg ctgtgtgcgc cttatccgct cctctgaact caagctcata
     2461 ggctcctgcg ctggtactta gcacaagctt gctgccggag aaaacgccgc gcccacccag
     2521 catccccgtg tcctccgagg ctgcgagggc atgaatgcgg tcgtaaggta cagagacgat
     2581 cgctttctct ttcttaatga aggcgctgtc ctggagcatc acgcgcttgt ccgtgatgcc
     2641 gacaaaacca gtgccgctcc cctttagatc gaagacagcg tagaccgtct cttccggcaa
     2701 caacccctct tgtatcttct ctagctgctc ttgattgtca aacattactt cttgttccat
     2761 gcatcccctt tccagctgga gttcatctcc tccagataac agggtagcat ggaagtctag
     2821 ccactctcaa cgatcaagct cctgagtgtc ctggtgaacc agggcaacgc acatatttgt
     2881 gcgggaattc ggattgcaca ttaattcagc ctcagagagc atttcttgtg caacgccgta
     2941 tcgggccact gttttcggat atggatcgcg gtcatgtcct cacccggcgg cgtcgaacac
     3001 agaggcctga cggctgatgc ctgccgtagg cgcagcctac ggcatcggct gtccccctgt
     3061 gacgccgagg gtctcgccgt tgacgtagct ggcttcctgg gaagcgagga agacgaaggc
     3121 cggggcgagc tcggcgggct gggccgggcg gcccatcggg gaggtgccgc cgaagctgga
     3181 gaccgtatcg ccgggcatgg aggcggggat gatcggggtc cacacgggcc cgggcgccac
     3241 ggtgttcgcc cttagcccgt actggacgat ctcctgggcg agtcctttgg tgaaggttac
     3301 gatggcgccc ttcgtcgagg agtaggggag aaggggcggc gaaggctgat aggcctggat
     3361 ggaggccacg ttgatgatcg aacctccctc cggcatgtgt gggagcgccg ccttgaccag
     3421 ccagaacatg gcgtagatgt tggtcttgaa ggtgcggtcg agcagctcgg tggagacgtc
     3481 tgctatcccg tccaccgtca tctggtgggc agcgttgttg acgaggacgt ctatccggcc
     3541 gaactcgtct acggccttct ggatgagcgc ccggcactgg gcctcatcgg ccacgtcccc
     3601 cggtgccttg acggccttgc ggccagagtc ttcgacggtc tgcgccgtct cttcggcgtc
     3661 ggcctcctcg tcttcgaggt aggagatcac gacgtcagca ccctcgcggg cgaaggcgag
     3721 cgcgacggcc cgcccgatac cggagtcgcc gccggttatg atggccttct tgccttcgag
     3781 cttaccactc ccgcggtagg tctcgacgcc gtagtccggc tcgggctgca tctcctgctc
     3841 caggcccggg tgctggtcgc gctggtcctg ctcggggtag tcgggctgcg ggtactgctg
     3901 cgtaggatcc tgttgcctgt gctggtcgtc tgccatcgct gtcgtccttc ctgttctgat
     3961 tgctgggttt cgcacctttt cccgttctat tctccgatca aacggcgagc ttccgaacgg
     4021 gttcaagaag gcggataata ggtgctggaa ggcggagagg ttctcacggg gaggaagcag
     4081 cttatgggag agttcggcat agtctttcag aacgagccgg tggacattag cgaggagttc
     4141 tctaagcagg agaaccactt cttcatcggc gacagggtcg aggagttcga ccctgcgagt
     4201 ggttccggga agatatgctg gaagagtctc tcgctcaagc aacgcgtctc gtaccatcag
     4261 atcacgctcc agctggagga ctacaaggtc tgggaggacc tgccacctga cgagtacgag
     4321 gacgaccgct cgctcccctt cgacctctct ttcgtctccc cacgcacggt tcgcctcagg
     4381 ctggccgcca gacctgaagg gtgcagcggg gaaccgacgc tcatgctcgc aggcgatcct
     4441 ccgtccgacg attcgtggga gacacatgat gagggatctt tcgtggcata ccatggcccg
     4501 tccggttccg tgatcgtagg aagagatccg ctgcgcttcg agttcaggga cgccgggggc
     4561 aaactcctga ccagcagcca ccacctctcc gacaccagtg gcgttgtcaa cgcctggccc
     4621 acacccttct cgttcgtccg caacaccgca acgttccaca ggcacatcgc ggccagcttc
     4681 ggcctctccc ccggagagaa gctcttcggc tgcggtgagt cctttacccg ccttgacaag
     4741 cgcggccaga agctggtcct gtggacctac gacgcctaca gcgcccagac gcccaacatg
     4801 tacaagccgg tccctttctt catgagcgac cgcggctacg gcatcttcgt gcacaccagc
     4861 gcgccgctca cgctcgacct cggcaactcc cacgacgggg ctgcggtgat ctacctcggc
     4921 gacgaccagc tcgacctgtt cttctttttc ggctcgccga aggagatcct ctcggagtac
     4981 accacgctta cgggccgctc ccccgtccct cccctgtgga ctttcggact ctggatgggc
     5041 cgccagacct actcctccga aaaggaggtc agggacgtgg cgaagaagct gcgcgaggat
     5101 cgtatcccat cggatgtgat ccaccttgac aaaggctggt ccgagatccc acaccgctgt
     5161 gacttcgagt tctcccactc ccgcttcgat gatccacaga agatgctcgc cgacctcgag
     5221 gaggaaggct tccgcatcag cctgtggcag ctaccttatt tcaaccccaa gaacaagcta
     5281 caccgagagg ccatagagaa ggatctcgtc gtcctgaccg cgagcggcga acctccggtg
     5341 gacgatgcga tcatcgacct gagcaagccc gaggccgatc gctggtaccg cgggaagctg
     5401 gcgaggttgc tggagatggg cgtcggcatc tttacggcgg acttcggcga ggccgcgcct
     5461 ctctccggca tgtacgcctc gaagaagggc ggcttccacg agcacaa
//
LOCUS       DATAIR010000833         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_85564, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000833 DATAIR010000000
VERSION     DATAIR010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85564"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VHM69_19845"
     CDS             complement(<1..321)
                     /locus_tag="VHM69_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2742703.1"
                     /translation="MSTRRTRKSPGTPEHNLPAPRSSFVGREREIEEVERELATTRLL
                     TLTGVGGSGKTRLALEVARDLLEAYPEGAWLVALAPLSEGPLVPKAVAEALGVPERPQ
                     EPLTD"
     gene            complement(1095..>3174)
                     /locus_tag="VHM69_19850"
     CDS             complement(1095..>3174)
                     /locus_tag="VHM69_19850"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2742704.1"
                     /translation="GGDPNEASSRLGAFLRGRKVLVIADDVFEPEHAAALKVLDRGTM
                     LVSTRFPAVEGALDVGPEDVYRLEPLGEDDSVALLRRQAPLLAGQDPGQIAGLARELG
                     GLPLFLKVAGRLLQEEAGYGRDAAGLLGDLRAGKEALLGERAPADVAGFDGARARTLE
                     VLMRASLEGLNEGERAAFRALGTMASKPATFDMEAAEAVWDSDEGRALMRPLVRRGLV
                     EADGKGRFSAHSVLGSFARFLLNEDSDGQGSYLRHVRHFVAVFARAGEAYYGDKEGLD
                     RAVRHLDADWQNILRGWSWAKSRDAEDVEAARLCFGYLEAGKRWPSLRLASQEHEHFA
                     RAAVGCARRLGDGPAEAGSLRRLGHALLRADRREEAKDACQRARSKYVEIGDEFGEGR
                     ACGALGACHKALGDAEEAERLYLEQIRTASGEDQTREEAASEAAGRGNLGVLYRDAGR
                     YEDAVREITAAAEIYDRIGDAREAVAARGNLGTVYHDQGDYDEAERRHLEFLRMARDL
                     GDKDSECRAVGNLGNVYRARGEPEKSLEHYEENLVLARGLGHLLFEGIALAGIARSRA
                     LLGESEAATAMHRQALQTIRSTGTPKIEAMILTDLAQLLRLQGEPHEALEHAAAAQSI
                     YEGLDFPPGEADAFFEAGLARYETGRRAAAVRDAERAREIYASLESPREREVEEQLRS
                     WREEEHAEIR"
BASE COUNT          487 a         1111 c          983 g          593 t
ORIGIN      
        1 gtcggtcaac ggctcttggg gacgctcggg cacgcctagc gcctcggcca cggccttcgg
       61 caccagcggc ccttcggaga ggggagccag cgccacgagc cacgccccct cggggtaggc
      121 ctcgagtaga tcgcgggcca cctccagggc gaggcgcgtc ttgcccgaac ctcctacccc
      181 ggtgagggtc aagagccgcg tcgtggccag ctctcgctcg acctcttcga tctctcgctc
      241 gcggcccacg aaactgctcc tgggcgcggg caggttgtgc tcgggggtgc cgggagactt
      301 gcgcgttctg cgggtgctca acgagtatcc tattcggtag tctggctgct cgaccgaagg
      361 cgagtcggcg acgtttctcg gtgcctgctc ggaggcagga tcctgtccct atcccggcgc
      421 tcgtgcgctc agcccccatc attgtagcaa tcgtcctaat ccgtcgtcgc gtagcacccg
      481 agcgctcaga tgcgcattat gcacggacag aagggatagt catgggccga atagaccgct
      541 ctaaggagtt acacctagtg aactcttgaa aacgccgttt ctaggcgaag tccggagaat
      601 tctccgaagt ccgaatacct gccgaatatg cgccccggtg aaagattatg cgatccgtgt
      661 tgcgaccggt ggcaatctca tgtaacgaaa cgcactacga cggtcagtta gtgtctctcg
      721 tcgatccttt agcgctttcg gagaatgtgt ccgacgcgtc gaacgtgccc agaaaggcca
      781 ttctatgcaa gacagtgatt tgcggacaat tggcgttctg cgaagttcac cggttgaaga
      841 tctcggaacc tcggatgcag cgtggtgcat agtcccggtc ggaccgggct aaggtcggac
      901 gaactctatg tgtcttacgt ccgccgtact ccccggcagt ccttctgagg gatggcctcg
      961 tccttgagaa gcaaggttac atccttctgc cgagtaggag atgaaagcgt gtcgatgaac
     1021 atcgtgggcc agcgacgctc gaaattatag tgccccgtgg aggttactga cgcacgtatg
     1081 ttgtctcggc caggctatcg gatctccgca tgttcctcct cgcgccagct gcgcagctgc
     1141 tcttcgacct ctcgttccct cggagactcg agcgacgcgt atatctcgcg cgcccgttcg
     1201 gcgtcgcgga cggcggcggc gcgccgcccc gtctcgtacc gggctaggcc agcttcgaaa
     1261 aaggcatcgg cctcacccgg cgggaagtcc agtccttcgt agatgctctg cgccgccgcg
     1321 gcgtgttcga gagcttcgtg aggttctcct tggagtctca gcagctgcgc taaatcggtc
     1381 agtatcatgg cctcaatctt cggcgtgccc gtcgatcgga tggtctggag agcttggcgg
     1441 tgcatggctg tggctgcctc ggactcgccg aggagggccc gggagcgggc gatgccggcg
     1501 agagctatgc cctcgaagag gaggtgcccg agcccgcggg ccagcacgag attctcctcg
     1561 tagtgttcta ggctcttctc aggctcgccg cgcgcgcggt agacgttgcc gaggttcccc
     1621 accgctcggc attcgctgtc cttatcgccc aagtcacggg ccatcctcag gaactcgagg
     1681 tggcgtcgct cggcttcgtc gtagtcgccc tggtcgtggt agacggtacc gaggttaccc
     1741 cgcgcggcga ccgcctcgcg cgcgtctccg atccggtcgt atatctccgc tgcggccgtg
     1801 atctcccgga cggcgtcttc gtacctgccg gcgtcgcggt agagtacgcc gaggttgccc
     1861 cgtccggccg cctcggacgc ggcctcctcg cgtgtttggt cctcgccgct ggccgtccgg
     1921 atctgctcga ggtacaggcg ctccgcctct tcggcgtcgc ccagagcctt atggcaggct
     1981 cccagcgccc cgcaggccct cccctccccg aactcgtcgc cgatctcgac gtatttgctt
     2041 ctggcgcgct gacaggcgtc tttggcctcc tcgcggcggt ccgccctcag gagcgcgtgc
     2101 ccgagcctgc gtaggctgcc cgcttcggcc ggcccgtcgc ccaggcgccg cgcgcagccc
     2161 accgcagcgc gggcgaagtg ctcgtgctct tgcgacgcga ggcgcagcga cggccaccgc
     2221 ttgcccgcct cgaggtaccc gaagcacagg cgcgcggcct cgacgtcttc ggcgtctcgc
     2281 gatttcgccc aggaccatcc tcgcaggatg ttctgccaat cggcgtcgag gtgccggacg
     2341 gcccggtcga gcccttcttt gtccccgtag tacgcctcgc ccgcccgggc gaagaccgcg
     2401 acaaaatgcc tgacgtgcct caggtacgag ccttggccgt cggagtcttc gttcaagagg
     2461 aacctggcaa acgaccccaa gacggagtgc gcggagaacc gccccttgcc atccgcctct
     2521 accagccccc ggcggaccaa cggccgcatc agggcccggc cttcgtcgga atcccagacc
     2581 gcctcggcgg cctccatgtc gaaggtggcc ggtttcgacg ccatggtacc cagagctcta
     2641 aaggctgcgc gctccccctc gtttaaaccc tccaagctgg cccgcatcag cacttcgagt
     2701 gtgcgtgccc tggccccgtc gaagccggcc acgtcggctg gcgcccgctc gccgagcagc
     2761 gcctccttgc ccgcgcggag gtctccgagc aaccccgccg cgtcccggcc gtagcccgcc
     2821 tcctcttgta gcaacctccc cgcaaccttg aggaaaagcg gcagcccccc aagctcgcgc
     2881 gcgagccctg cgatctggcc cggatcttgc cccgcaagta agggtgcctg cctacgcagc
     2941 agagccacgg aatcgtcttc gcccaacggc tccaggcggt agacatcctc cggccccacg
     3001 tcgagtgcgc cctcgactgc cggaaatcgc gtgctcacga gcatggtccc gcggtcgagc
     3061 acctttagcg ccgccgcgtg ctcgggctcg aagacgtcgt ccgcgatcac cagcaccttc
     3121 ctgccccgca ggaaggcccc cagccgcgag gaagcctcgt tagggtcccc accg
//
LOCUS       DATAIR010000834         5333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_181215, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000834 DATAIR010000000
VERSION     DATAIR010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181215"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VHM69_19855"
     CDS             complement(<1..765)
                     /locus_tag="VHM69_19855"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2742705.1"
                     /translation="MLADLKLSRPIMTGVATYPAGATFGPRATRDFEFVWVIEGDVEY
                     SRDGMIVPAPPGSVLLCQPGGTDFFKWDEKRRTRHGYFHFKVLSLPDSWPPMQEWPLI
                     HPPVDGDILRPLFRHLLTWADRGDPNLLAVTMAHMLASFVTGETAGRDVPQESLPEAV
                     ERALTYLHDSLEEDPSTSIHLADLAGAACVSPEHLCRLFKRSTGRSPVETVRLARLDR
                     AAVLLARSNYTVAEISTVCGFSSPFHFSRRFKDAFGL"
     gene            complement(786..1643)
                     /locus_tag="VHM69_19860"
     CDS             complement(786..1643)
                     /locus_tag="VHM69_19860"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2742706.1"
                     /translation="MKQESITTDLDEAPGFRIWRGEPLATMPFHWHDQIEINYSVGGS
                     LTYLLAGSMVRVPPGRLALFWAGMPHSVVSRTEVESFYWVYLPLVWALRLGLPTAFTR
                     RVMSGQMILDAAPSGTDLPMLDRWSDELPHADEARRRLIVREVETRVMRMALDQTDEP
                     DCEHVPTEAGPLRKVSEMARFVAENHAGPLRLENVARHVGLHPNYAMTLFRRHYGITL
                     STYVTRLRVCQAQYLLLSTDQEVSRIAFETGFGSISRFYEAFKAVSGRTPRQYRLLPI
                     PPHNSKPLP"
     gene            1955..3247
                     /locus_tag="VHM69_19865"
     CDS             1955..3247
                     /locus_tag="VHM69_19865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2742707.1"
                     /translation="MSGTGALGVGLLGVYGCGGGGGGSSTGQVAWASWANSGEAERFR
                     EYTDHFMEEYPDIQVTYTPTPNYDDYHPKILTQLNGGTAPDAFYAGDIWVAQFIKNGT
                     ISKLNDLMTGSNSEAQPDDFAAGLWGPARTSEGTIYGITVDCNPTVIWYNKGVLEEAG
                     ISDDPIALYESGEWKWDVFQQMCEKVASKGGLNGFMFENGNSWFYSWCRQNGGTPYDG
                     DTCVVNEDPKSVAAFQWMYDNISSEAFTYAGNLPTGQTSDAQFNSSRAAFIEAGRWLL
                     PVFKENNTLEYDVVPFPTNTDNDMEPGWVATAYMVMNKKTASVKDTFTFITNFTSAAG
                     QKYRLEEAGNAVPSVLKGADDVVTKDTTLEGAPYFLDIRDTGIVGYAELSVPGLVEDM
                     QDVLENELWLPSGDGDVQGALDEIAKMANEGIKKNSTE"
     gene            3323..4237
                     /locus_tag="VHM69_19870"
     CDS             3323..4237
                     /locus_tag="VHM69_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2742708.1"
                     /translation="MSSAVKASGSRLSKRRKSDIKWGYFFIFPQLFGVVAFVLFPLIY
                     VLVLSLLEWDGLGEASFVGLSNYTTELTHPDFRNALSNTVYYTVMVVPTSVVLSLMAA
                     LALNKLRLKNFFRVIYFAPVVTSTVAAGVVWLWVYNPDFGLINTYLDTWFGIKGPQWL
                     LDPKWVMPSISIMSIWLRMGFYIVIFLAGLQGIPVTYIEAAQIDGANRLQTFWYVTLP
                     LLSPTTFFIFIIAIIDSFQVFDQAFILTFDGGPANSAVTMVYYLRELAFVDFVYGRSA
                     AVAVILFAVILAFTLMQFRVQRRWVHYD"
     gene            4265..5146
                     /locus_tag="VHM69_19875"
     CDS             4265..5146
                     /locus_tag="VHM69_19875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2742709.1"
                     /translation="MASVATTKGTAGPVETTSRTSRIVGTVLLYLAVTVGSLVMVLPF
                     AWMLLGSVKDLGQLYRVPPNWIPDPFVFQNYVNAWTSVPFDTGYINSTIVTVSVVLVN
                     LLTCSMAAYAFARIEFPFRHVLFILFLATLMVPEQVTIIPLYIIIKNVGLIDTLLSLI
                     IPYALFNAFGVFLLRQFIKGLPIDLEEAAIVDGANRWTIYWRIILPLIRPALAAFGIF
                     TFLFQWNNFFRPLIFLNSIENYTVPLAINFFRGQYATDFALLMAGSAISIVPVLIVYI
                     IGQRHIIEGIATTGLKQ"
BASE COUNT         1045 a         1592 c         1648 g         1048 t
ORIGIN      
        1 gaggccgaag gcgtccttga agcggcggga gaagtggaag ggactggaga agccgcagac
       61 cgtggagatc tccgccaccg tgtagttgct acgcgccagg aggacggcgg ccctgtcgag
      121 gcgggccagg cgcacggtct ccacagggga ccttccggta gacctcttga acaggcggca
      181 caggtgttca gggctcacgc aggcggcgcc tgcgaggtcc gcaaggtgga tacttgtcga
      241 agggtcctcc tccaggctgt cgtgtaggta cgtcagggcc cgttcgacgg cttcgggcag
      301 ggactcttgc ggcacgtccc tgcccgcggt ctcgccggtt acgaagctcg cgagcatgtg
      361 tgccatcgtc acggcgagca gattaggatc gccccgatcc gcccacgtga gcaggtgccg
      421 aaagaggggc ctcaggatgt cgccatcgac gggagggtgg atcaggggcc attcctgcat
      481 ggggggccag ctatcaggga gggagaggac cttgaagtgg aagtacccgt gacgcgtgcg
      541 gcgcttctcg tcccacttga agaagtccgt cccgcctggt tggcagagca gcaccgaccc
      601 tggcggagcc ggtacgatca tgccgtctcg actgtactcc acgtcgcctt cgatgaccca
      661 cacgaactcg aagtcgcgcg tggcccgcgg gccgaaggtc gcgcccgctg gatacgtggc
      721 gacgcccgtc attatgggcc gcgagagttt caggtcagcg agcatctcct accaggcaca
      781 tcccgtcatg ggaggggttt cgagttatga ggtggaatcg gcagcaatct gtactggcgg
      841 ggcgtccttc cgctcactgc cttgaacgct tcatagaagc gactgatcga cccgaagccg
      901 gtctcgaacg cgatgcgcga gacctcctga tcggtggaga gcagcaggta ctgcgcctgg
      961 cagacgcgca gccgcgtcac gtaggtactc agcgtgatcc cgtagtgccg ccggaacagc
     1021 gtcatcgcgt agttgggatg gagtccgacg tgccttgcca cgttttctag tcgcaacggc
     1081 ccggcatgat tctcggcgac gaagcgggcc atctcgctta cctttcgcaa tggccctgcc
     1141 tccgtaggaa catgttcgca gtccggctcg tcggtctgat ccagggccat gcgcatcacg
     1201 cgcgtctcga cctcacggac tatcaggcgg cggcgggctt cgtcagcgtg tggtagttcg
     1261 tcgctccagc ggtcgagcat cgggaggtcc gtaccggaag gggcggcgtc gaggatcatc
     1321 tggccgctca tgacacgcct ggtgaacgcg gtcggcaacc cgaggcgcaa ggcccacacc
     1381 agcggcaggt agacccagta aaagctctct acctcggtcc tgctcacgac gctgtgcggc
     1441 atccccgccc agaacagcgc cagcctgccc ggtgggaccc ggaccatcga tccggcgagc
     1501 aggtaggtga ggctccctcc gaccgagtag ttgatctcga tctgatcgtg ccagtgaaac
     1561 ggcatcgtgg cgagcggctc tccgcgccag atccgaaacc ccggtgcctc gtccaggtcg
     1621 gtagtgatgc tctcctgttt cacccggacc ttagtttacc ggaatacgtg gcccaatatt
     1681 cggaagaact cggaagagtt catccgtaaa gttcacaggt ggtaagtgga aaggagtagc
     1741 atcggggata cggaagcagg tggcaaacac gtttgcacga cgacgtgcgg attcttcgcc
     1801 gtgcgacgtg tgcctgcctt cttgctactc aatttacgtt gacccgttga gtcgggtggt
     1861 ggtacgatta cacacgaatc gacacaaatc ctgggggaaa gggtaaatac agatgaatgc
     1921 gggtagccgg ctttcgcggc gggatttcct gaagttgagt ggtaccgggg cgttgggcgt
     1981 tggcttgctc ggtgtctacg ggtgcggggg gggtggcggc ggttcctcta cggggcaggt
     2041 cgcctgggct tcctgggcca acagtggtga ggccgagcgt ttcagggagt acacggacca
     2101 tttcatggag gagtatcccg acatccaggt cacctacacg ccgactccca actacgacga
     2161 ctaccacccc aagatcctga cccagctcaa cggcggcacc gcccctgacg cgttctacgc
     2221 gggagacata tgggtagccc agttcatcaa gaacgggacc ataagcaagc tcaacgacct
     2281 catgacgggc tccaacagcg aggcccagcc cgacgatttc gccgccgggc tgtgggggcc
     2341 tgccaggacc agcgagggga ccatctacgg cataaccgtc gactgtaacc ccaccgtcat
     2401 ctggtacaac aaaggagttc tcgaggaggc ggggatcagc gacgatccca tagccctcta
     2461 cgagagcggc gagtggaagt gggacgtctt ccagcagatg tgcgagaagg tcgcttccaa
     2521 gggcggcctc aacggtttca tgttcgaaaa cggcaacagc tggttctact cgtggtgccg
     2581 gcagaatggt gggacgccct acgacggcga cacctgcgtc gtcaacgagg atcccaagtc
     2641 ggtagccgcc ttccagtgga tgtacgacaa tatcagtagc gaagcgttca cgtacgcagg
     2701 caacctcccg acagggcaaa cgtccgacgc ccagttcaac tccagccgcg cggcctttat
     2761 cgaggcgggc cggtggctcc tgcccgtctt caaagagaac aacacccttg agtacgacgt
     2821 ggtccccttc ccaaccaaca cggacaacga catggagccg ggatgggtcg cgacggctta
     2881 catggtgatg aacaagaaga cggcgagcgt caaggacacg ttcacgttta tcaccaactt
     2941 cacctccgcg gcgggacaga agtacaggct tgaggaggcg ggcaacgccg tgccttcggt
     3001 gctgaaaggc gcggacgacg tcgttaccaa ggacaccacg ctggaaggcg ccccctactt
     3061 cctcgacatc cgcgacacag ggatcgttgg ctacgccgag ctctccgtgc ctgggctcgt
     3121 cgaggacatg caggatgtac tggagaacga actatggctg cccagtggcg acggggacgt
     3181 gcagggagcc ctcgacgaga tagcgaagat ggcgaacgag gggatcaaga aaaactcgac
     3241 cgagtagtgg tagtcgggtc ccaggggtgg gaggaacgct cctcccacct gagggcagac
     3301 catctcgaga aggggaacgc aagtgagtag cgcggtcaag gcgtcggggt ccaggctcag
     3361 caagaggcgc aagagcgaca tcaagtgggg atatttcttc atctttccgc agctcttcgg
     3421 ggttgtggct ttcgtgctgt tccctttgat ctacgtgctc gtgctaagcc tgctggaatg
     3481 ggatgggttg ggcgaggcca gcttcgtggg cctgtcgaac tacacaaccg agctgaccca
     3541 ccccgacttc aggaatgccc tgagcaacac cgtctactac acggtgatgg tggtgccaac
     3601 cagcgtggtg ctctcgttga tggccgccct ggcgttgaac aagcttcgcc tcaagaactt
     3661 cttccgggtg atctacttcg cccccgtcgt caccagcacg gttgcggctg gcgtcgtgtg
     3721 gttgtgggtc tacaaccccg acttcgggct catcaacacc tacctggata cctggttcgg
     3781 catcaagggt ccgcagtggc tgctcgaccc caagtgggtg atgccctcga tctcgataat
     3841 gagcatctgg cttaggatgg ggttctatat cgtcatcttc ctggcagggc tccagggtat
     3901 ccccgtaacc tacattgaag cggcccagat agacggggcc aacaggctcc agaccttctg
     3961 gtacgtcacg ctcccgctgc tttcgcccac aacgttcttt atctttatca tcgctattat
     4021 cgactcgttc caggtcttcg accaggcctt catccttacc ttcgacgggg gcccggccaa
     4081 ctcggccgtc accatggtct actacctcag ggagttggcg ttcgttgact tcgtctatgg
     4141 caggagcgcc gctgtcgccg tgatcctgtt cgcggtcatc ctggctttca ccttgatgca
     4201 gttcagggtc cagcggcgat gggtccacta cgattgagtt tctaccggga aaggagggag
     4261 cataatggca agcgtggcga caactaaagg cacagccggc ccggtagaga ccacgagccg
     4321 tacgagccgt atcgtgggga cagtgctgct gtacctggcc gtgacggtgg ggtcgctcgt
     4381 gatggtcttg cccttcgcgt ggatgttgct cggctcggtc aaggacctcg ggcaactata
     4441 cagggtgccg cccaactgga tccccgaccc attcgtgttc cagaattacg tgaacgcgtg
     4501 gacttcggta cccttcgata cgggctacat aaatagcacc atcgttacgg tttcggtggt
     4561 cctggtgaac ctgctcacgt gctcgatggc ggcatacgct ttcgccagga tagagttccc
     4621 cttcaggcac gtgctcttta tcctgttcct ggcgacgctc atggtccccg agcaggtcac
     4681 gatcatccca ctttacataa tcataaagaa cgtgggtctc atcgatacgc tcctgtcgct
     4741 gatcatcccc tacgcgctct tcaacgcctt cggcgtcttc ctactcaggc agttcataaa
     4801 aggtcttccc atcgacctcg aggaagcggc gatcgtggac ggtgcgaacc gctggacgat
     4861 ctactggcgg atcatccttc cgctcatcag gccagccctg gcggctttcg gcatcttcac
     4921 gttcctgttc cagtggaaca acttcttcag gcccctgatc ttcctgaaca gcatcgagaa
     4981 ctacacggtg ccactggcga tcaacttctt ccgcggccag tacgccacgg acttcgctct
     5041 tctgatggcg ggctcggcga tatccatcgt ccctgtcctg atcgtgtaca tcatcggcca
     5101 gcgccacatc atcgagggta tagccacgac cggcctgaag cagtagacgc gccgcagcga
     5161 aggtgctgaa ccggcccgta cgctagcctg gcctgagatc cagggggaga tcagaacttt
     5221 gcagccgaac gaatgcggcc acctggacac ccatttcagg gaaccagtcg tggaggagaa
     5281 cgtttttgta ggaatgtctc ccgagaacgt gccgttgccc tcgttcgacg aga
//
LOCUS       DATAIR010000835         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_183139, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000835 DATAIR010000000
VERSION     DATAIR010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183139"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1034
                     /locus_tag="VHM69_19880"
     CDS             63..1034
                     /locus_tag="VHM69_19880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2742710.1"
                     /translation="MIEFKNVSKTYAGSDRSVVNDLTFTIQNGEICVLVGPSGCGKTT
                     SMRMVNRLIEPTKGEILIDGQKNTSMSGTQLRRKIGYAIQQIGLFPHRTIASNVATVP
                     NLVGWESDRVASRVDELLDLVGLPPDQYRDRYPAELSGGQQQRVGVARALAADPPIML
                     MDEPFGAVDPITRDRLQNEFLNIQKDIKKTIVFVTHDIDEAIKMGDKIAILKEGGVLA
                     QYDSPEHILANPASDFVSSFVGTDRVLKRLSLVRVRDMDLDPVNGGDNLPRISDKLTL
                     KDALSEIIGAGAERGLVVPDGNGGNPRGTLSIDAIRTLSHDAEVANG"
     gene            1027..1764
                     /locus_tag="VHM69_19885"
     CDS             1027..1764
                     /locus_tag="VHM69_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742711.1"
                     /translation="MDSEDPPTAILAFLQAEFATGEQKEVGLIDWEWLADNFREDVLP
                     AFLQHIYLCFVSLAIAVAISVPLGILAARYRSLYPPLTAITGFFYTIPSFSMFTILIF
                     VLGFSVGKTPAIIALVLYSLLILIRNVVTGLDSVPPETKDAARGMGLTDRQILFRVEL
                     PLALPVIVAGMRIAIVTVIGIAVIGAYIGAGGLGDLIFDGITRDFPTLYITGAVLATL
                     LAIGSDLLFVRAERMLSPWARRSRGAT"
     gene            1766..2425
                     /locus_tag="VHM69_19890"
     CDS             1766..2425
                     /locus_tag="VHM69_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2742712.1"
                     /translation="MNEFMGVLQRPDFVDNLILHIEYLFLATHLGILIAIPVSVLVYR
                     SDWASTFAINVGNVGRAVPSLAVLVLMFPIFGLGFDSALFALTALAIPPIMINAIVGL
                     RQVSPQILDSAKGMGLSGQQILSRIQVPIAAPIIFAGIRTSAVQVVASATLATFIGGG
                     ALGDYIVEGYQRNNTAIALAGALSVAVLAIITEVGFAGLERTFTPKGLRIAQKRGRAS
                     K"
     gene            2437..3387
                     /locus_tag="VHM69_19895"
     CDS             2437..3387
                     /locus_tag="VHM69_19895"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2742713.1"
                     /translation="MKDRNMLIRAVTALLVTALLVVTAACGGGGGGGGESSSGPSVSV
                     GSKKFTEQILLGEMYAQAFEDEGYDVERNLNLGSEQVMDKSLQDGTIDVYPEYTGTAY
                     VAILKKPPESYPKTEKETYEQVAKFYKNRKDTPMQMLQPAPFENNYGIVMLTKEANEM
                     GIETLEDLAAQSDELIFSSYSEFQNRSDGYDNMKKHYPKLDFKEIKIVNDLGIRYKAL
                     AEGEADVGIGFTTDGQLASPKLKVIEDTEDIWPKYYPAPVVTQSFLDKNKDAEKILNE
                     VSSSLNADKMRQLNGAVDLEQKDYEDVARQHLEDEGVIEN"
     gene            3478..4203
                     /locus_tag="VHM69_19900"
     CDS             3478..4203
                     /locus_tag="VHM69_19900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2742714.1"
                     /translation="MLRGKGEYRIERWLAGGVPLATVRSSAARAPLPAVVVYHGFAGK
                     KTDDLLSLAVPLADAGCLAVMPDAALHGERAPHDFALRRAEEHDTLFLETLRGTVEES
                     EGVLQWILDREEVDEGRIGAVGISMGGAVVLALACRKLSNPLRAAVALMPAIPGPGSV
                     VRQEAAYAPDPAECFPTPLLMIHGTADQAAPYPNARHFYDGLVPAYSQAPERLRFIDV
                     PGEEYRIGTYWVEETLAWLGRFL"
     gene            complement(4384..5982)
                     /locus_tag="VHM69_19905"
     CDS             complement(4384..5982)
                     /locus_tag="VHM69_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX2742715.1"
                     /translation="MAAEDLLTIYHPTTGTVTLNGDDLSIEEVVRIARRGARVEVSRE
                     ALDRVRRARDVVDRVLERGDLVYGMNTGVGSLSRYRIPFESLEQFSVRLVTRHTTHQG
                     TELAPEVVRAMMVTRANGMAKGGVGVRAELIQAFLDALNAGVHPVVRTGGSVGQADLA
                     EMAEIGQVLIGRGEADHKGLRLPGAEALHAAGLQPIRLKAKEGLALISTNGVTMGHGS
                     LVLADVADLLDAFDVTAALSLEAFGANLSTIHPEAARMRPHPGQARAADRLRELLAGS
                     YLWRPGAARNLQDPLSFRCVPQTHGACYDAHAYSRGTMEVELNSASDNPLVLLDDQSI
                     ISVGNFDVVALAVAFDLLRIAVAQVVHLANERIQKHLWSQFSGLPTGLAARDGDEGLR
                     PLGYTSASLAAEARVLANPVSLDYRGQIAEGFEDHASMAPLGVRKTEELLSISRRMAA
                     LELTVSTRAIDLRGGPPLGAGTGKACDIAREFATADPEERWPDVDGLTEVIDSGRLVA
                     RVAAEIGPLEPVSTPGDAVGPAEG"
     gene            6055..>7350
                     /gene="hutU"
                     /locus_tag="VHM69_19910"
     CDS             6055..>7350
                     /gene="hutU"
                     /locus_tag="VHM69_19910"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2742716.1"
                     /translation="MTDTRSAPTIRAPRGRDLTCKGWHQEAALRMLMNNLDPEVAERP
                     EDLVVYGGTGKAARNWQSFWAIVSTLQGLDDDETLLVQSGKPVAVFKTHPLAPRVLIA
                     NSLLVPEWADWKTFRELERAGLTMYGQMTAGSWIYIGTQGILQGTYETFAAIAGQRFG
                     GTLRGRVCLTAGLGGMGGAQPLAITMNDGVALCVEVDPDRIERRISHRYLDERIDDLD
                     EAVKRAEEAKHAGEPLSIGILGNAAEVFPALLERGYVPDAVTDQTSAHDPLVGYVPAG
                     YDLEAAAELRESDPERYISEARASIARHCAAMVGFKERGSEVFDYGNSLRGEAKLGGF
                     EGAYGYPGFVPAYIRPLFCEGRGPFRWAALSGEESDIAATDDAILELFPHDERLERWI
                     SNAREKVHFQGLPARICWLGAGERHKAGLRFNELVADGAI"
BASE COUNT         1365 a         2376 c         2317 g         1292 t
ORIGIN      
        1 ggcaatggag gcaaggaagg cttactgggt agatttctat ccatggatcg gctggagaac
       61 ctatgatcga gtttaagaac gtaagcaaga cctacgccgg ctcggacagg tccgtggtca
      121 acgacctcac cttcacgata cagaacgggg agatctgcgt ccttgtaggt ccttccgggt
      181 gcggcaagac gacgagcatg aggatggtca accgcctcat cgagccgacg aagggtgaga
      241 tcctcatcga cggccagaag aacacttcga tgagcggcac ccagctacga cgcaagatcg
      301 gctacgcgat ccagcagata gggctcttcc cccaccgtac tattgcgagc aacgtcgcca
      361 ccgtacccaa cctggtcggg tgggaaagtg accgtgtcgc gagccgcgtg gacgagttgc
      421 tcgacctcgt cggcctgcct cccgaccagt atcgcgaccg ttaccctgcg gagctctccg
      481 gcggccagca gcagcgcgtc ggggtggcac gggcgttggc cgcggacccc cccatcatgc
      541 tcatggacga gccgttcggc gcggtcgacc cgatcacacg cgacaggctg cagaacgagt
      601 tcctcaacat ccagaaagac atcaagaaga ccatcgtctt cgtcacccac gacatcgacg
      661 aggctataaa gatgggcgac aagatcgcca tcctcaaaga gggcggcgtc ctggcccagt
      721 acgatagtcc cgagcacatc ctggccaacc cggcctcgga cttcgtatca tccttcgtcg
      781 gcacggaccg ggtcctcaag cggctctcgc tcgtgcgggt gcgcgacatg gaccttgacc
      841 ccgtgaacgg aggcgacaac ctcccgcgca tcagcgacaa gctcacgctc aaggacgccc
      901 tctccgagat catcggcgcc ggcgcagaac ggggcctcgt cgtgcctgac gggaacggcg
      961 ggaacccgcg cggcacgctc tccatcgacg ccatcaggac cctctcgcac gacgcggagg
     1021 tggcgaatgg atagcgagga ccctccgacc gccatcctgg cgtttctcca ggccgagttc
     1081 gccaccgggg agcagaagga agtcggcctc attgactggg agtggctcgc cgacaacttc
     1141 agggaagatg tattgccggc cttcctccag cacatctacc tgtgcttcgt ttcgctggcg
     1201 atagcggtcg ccatctcggt gccgctcggc atactcgcgg cccgctaccg gtcgctctac
     1261 ccgccgctga cggccatcac gggattcttc tacacgatcc cgagcttctc catgtttacg
     1321 attctgatct tcgttttggg tttcagtgtc ggcaagacgc cggcgatcat cgcgctcgtg
     1381 ctctactcac tgctcatcct tattcgcaac gtggtgaccg gcctcgactc cgtgccgccg
     1441 gagacgaaag acgccgcaag ggggatggga ctgacagaca ggcagatact cttcagggtc
     1501 gagttgccgc tggcgttgcc ggttatcgtg gccgggatgc gtatagccat agttacggta
     1561 atcggcatag cggttatcgg cgcgtacatc ggtgccgggg gacttggcga cctgatcttc
     1621 gacgggataa cccgcgactt cccgaccctg tacatcacag gggcggtcct tgcgaccttg
     1681 ctcgccatcg gctccgacct cctgttcgtc agggcggaac gtatgttgag cccgtgggcg
     1741 cgccggtcga ggggggcgac gtaagatgaa cgagtttatg ggagttctgc aacgccccga
     1801 ctttgtcgat aaccttatcc tgcacatcga gtaccttttt ctcgcgacgc accttggcat
     1861 cctcatcgcc atccccgtgt ccgtactcgt gtaccgttcg gactgggcct ccacgttcgc
     1921 catcaacgtt ggcaacgtgg ggcgcgcggt gccttcgctc gccgtgctgg tcctgatgtt
     1981 ccctatcttc gggctcgggt tcgacagcgc gctcttcgcg ctgacggcac tcgccatacc
     2041 accgatcatg ataaacgcca tcgtcggcct gcggcaggtg agcccgcaga ttctggactc
     2101 cgcgaagggc atgggtctct cgggccagca gatactttcc aggatacagg tgccgatagc
     2161 cgcgcccata atcttcgccg gcatcaggac ctctgccgtg caggtggtgg ccagcgcgac
     2221 cctggcgacg tttatcggcg gcggcgccct tggcgactat atagtcgagg ggtaccagcg
     2281 caacaacacc gccattgcgc tcgcaggggc cctctccgtg gcggtgcttg cgatcataac
     2341 cgaggtagga ttcgctgggc tggagaggac gttcacgccg aaggggctcc gtatagccca
     2401 gaagcgaggc agagcaagca aatgacagga ggaagagtga aggacaggaa catgttgatc
     2461 cgggcggtaa cggcactgct cgtcaccgcc ctgctagtcg tcacggcggc ttgcggcgga
     2521 ggaggcggtg gcggcggcga gagcagcagc ggcccgagcg tctccgttgg ctcgaagaag
     2581 ttcaccgagc agatactcct cggcgagatg tacgcccagg cgttcgagga cgagggctac
     2641 gacgtcgagc gcaacctgaa cctcggctcc gagcaggtca tggacaagtc cttgcaggac
     2701 ggcacgatag acgtttaccc cgagtacacg ggcacggcct acgtggccat tttgaagaag
     2761 ccgcccgagt cgtacccgaa gacggagaag gagacctacg agcaggtcgc caagttctac
     2821 aagaaccgca aagacacccc gatgcagatg ctccagcccg ctccgttcga gaacaactac
     2881 ggcatcgtga tgctgaccaa ggaagccaac gagatgggca tagaaactct ggaggacctc
     2941 gcggcacagt ccgacgagct catcttctcg tcctactccg agttccagaa tcgctctgac
     3001 ggatatgaca acatgaagaa gcactacccg aagctcgact tcaaagagat caagatcgtc
     3061 aacgacctcg gcatccgcta caaggccctg gccgagggtg aggcagacgt cggtatcggg
     3121 ttcacgacag acggccagct cgcctccccc aagctgaagg tcatagagga caccgaggat
     3181 atctggccga agtactatcc tgcccctgtc gtcacccagt cgttcctgga caagaacaag
     3241 gacgccgaga agatactgaa cgaagtctcc tccagcctga acgcggacaa gatgcgtcag
     3301 ctcaacggcg cggtggacct cgaacagaaa gactacgagg atgtcgccag gcaacatctc
     3361 gaagatgagg gcgtcataga aaactaggcc accgggagag aactactaga gcgctttaca
     3421 gagccgccgt ggtgtacacc acggcggctc cttctttaca ttcgagggga acagaggatg
     3481 ctacgtggca agggagagta taggatcgag cggtggctgg ccggcggagt accgctcgcg
     3541 accgtgcgtt catccgcggc gcgggcgccg cttcctgctg tggtcgtcta ccacggcttc
     3601 gcaggcaaga agaccgacga cctcctgagc ctcgccgtcc cgctagcgga cgctggatgc
     3661 ctcgccgtca tgcccgacgc tgccctgcac ggcgagcgag ccccacacga cttcgccctc
     3721 cgccgcgccg aagaacacga caccctgttt ctggagaccc tgcgaggtac cgtcgaggag
     3781 tccgaaggcg ttctgcaatg gatcttggac cgcgaagagg tggatgaggg caggatcggg
     3841 gccgtgggaa tctcgatggg cggcgccgtc gtgctcgctc ttgcctgccg caagctctcc
     3901 aatcccctgc gggctgccgt cgccctcatg ccagccattc caggaccggg cagcgtcgtg
     3961 cggcaggagg ctgcctacgc ccctgacccg gcggagtgct tcccaacacc attgctcatg
     4021 atccacggca ccgcagatca agccgcaccc tacccgaacg cccgccattt ttacgatggt
     4081 ctggtcccgg cttactctca agcccctgag cggctgcgct ttatagacgt gccgggcgaa
     4141 gagtaccgca tcggcaccta ctgggtagag gagaccctcg cctggctcgg gaggtttctt
     4201 tagaggtttc aggtccttgc tggagatact ccttcgtgtg aatccgcccc tggcagattc
     4261 gagacacacg acgtacctct aaagtggtac taaatacgtc caaccaggtt cacctctcgg
     4321 cacgctgcta tcggtcagcg gtagtgcccg gagatcagga tactgaaacc tgatgccttt
     4381 ttttcaccct tcggcgggtc caacggcgtc gcctggggtc gagacgggct cgagcggtcc
     4441 gatctcagcc gccacgcgcg cgactagcct gccgctatcg attacctccg tcagtccgtc
     4501 tacgtcaggc cagcgctcct cggggtcggc agtggcgaac tcgcgcgcta tatcgcacgc
     4561 tttccctgtt ccagccccga gtggcgggcc gccgcgcagg tcgatggcgc gggtggagac
     4621 ggtcagttcc aacgcggcca tgcggcgtga gatcgagagt agctcctctg tcttgcgtac
     4681 gccgaggggc gccatgctcg cgtggtcttc gaacccttcg gcgatctggc cccggtaatc
     4741 gagcgagacg gggttggcga gcacccgcgc ctcggccgcg agagacgccg acgtatagcc
     4801 cagtggcctg aggccctcgt cgccgtcgcg tgctgcgagg cccgtcggca acccgctgaa
     4861 ctgcgaccag aggtgtttct ggatgcgctc gttggcgaga tggacgacct gggcgaccgc
     4921 tattcgtaac aggtcgaagg cgacggcgag ggcgactacg tcgaagttgc cgacggagat
     4981 gatggactgg tcgtcgagca ggacgagcgg gttgtcgctc gcggagttga gctcgacctc
     5041 catggtgcct cgggagtagg cgtgggcgtc gtaacaggct ccgtgggtct gggggacgca
     5101 cctgaaggag agcgggtcct gcaggttgcg cgcagcgcca ggacgccaca ggtaactgcc
     5161 cgccagaagc tcccgcagcc tgtccgcggc ccgcgcctgc cctgggtgtg gccgcatcct
     5221 ggccgcctcg gggtggatgg tcgagaggtt cgccccgaaa gcctccagcg agagcgccgc
     5281 cgtcacgtcg aacgcgtcga gcaggtcggc cacgtctgcg aggacgagcg acccgtggcc
     5341 catcgtcacg ccgttggtgc ttatgagcgc cagtccctct ttcgctttca ggcgtatggg
     5401 ctggaggccg gcggcatgca gggcttccgc accaggcagc ctcaagccct tgtgatcggc
     5461 ctctccacgt ccgatgagta cctgccctat ctccgccatc tcggccaggt cggcctggcc
     5521 cacggatccc cctgtacgca cgacgggatg gacgccggcg ttcagagcat ccaagaacgc
     5581 ctgaatgagc tcggcccgca ccccgacgcc gcccttcgcc atgccgttcg ccctggtgac
     5641 catcatcgct cgcacgacct cgggggcgag ctccgtcccc tggtgggtag tgtggcgcgt
     5701 gaccagccgc acggagaact gctccaggct ctcgaagggt atccggtagc gggagagtga
     5761 accgacgccc gtgttcatgc cgtagacgag gtcgccgcgc tcgaggaccc tgtccacgac
     5821 gtccctggcc cgtctgacac ggtccagggc ttcgcgggag acctccacgc gcgcgccccg
     5881 cctggcgatg cgcaccacct cctcgatgga gaggtcgtcg ccgttgaggg tcacggttcc
     5941 ggttgtcggg tggtagatcg tcaacaagtc ctccgccgcc atattactct cccacactgg
     6001 actttataag gagccggtga cagactggat cccgatttca tggaaggagt ccccatgacg
     6061 gacaccagaa gtgccccaac catccgagcg ccgcgaggga gggacctcac gtgcaagggc
     6121 tggcaccagg aggcggccct gcggatgctc atgaacaacc tcgaccccga ggtggccgaa
     6181 cgtcctgaag acctcgtcgt ctacggcggg acgggcaagg ccgccaggaa ctggcagagc
     6241 ttctgggcca tcgtgagtac tctgcagggc cttgacgacg acgagacgct gctcgtgcag
     6301 tcgggcaagc ccgtcgccgt cttcaagacg cacccgttgg cgccgcgggt cctgatcgcc
     6361 aactccctcc tcgtccctga atgggccgac tggaagacgt tcagggagct ggagcgggcg
     6421 gggctcacga tgtacgggca gatgaccgct gggtcgtgga tctacatcgg cacccagggc
     6481 atcctccagg gcacctacga gacgttcgcc gctatagccg ggcaacgttt cggcggtacg
     6541 ctgcgcggcc gcgtctgcct cacagcgggc ctcggcggca tgggaggagc ccagccgctc
     6601 gccatcacca tgaacgatgg cgttgccctc tgcgtcgagg ttgacccaga caggatcgaa
     6661 cgccgcatct cccaccgtta cctggacgaa cggatagacg acctcgacga ggccgtcaag
     6721 cgcgccgagg aggcgaaaca tgccggagag ccgctctcca tcggcatcct cggcaacgcg
     6781 gcggaagtat ttccggcgct attggaacgc ggctacgtcc ctgacgccgt cacggaccag
     6841 acttcggccc acgacccgct cgtcggctac gtcccagcag gctacgacct ggaagcggcg
     6901 gccgagttgc gcgagtccga ccccgagcgc tacatctccg aggcccgcgc ctcgatagcc
     6961 cgccactgcg ccgctatggt cggtttcaag gagcgtggct ccgaggtctt cgattacggt
     7021 aactctctgc gcggggaagc gaagctcggc ggcttcgagg gcgcttacgg ctaccccggg
     7081 ttcgtgcctg cgtacatcag gccacttttc tgcgagggca gggggccttt tcggtgggcc
     7141 gcgctctcgg gcgaggagtc tgacatagcg gcgacggacg atgcgatcct ggagctcttc
     7201 ccccacgacg agcgtctgga gcgttggatc tccaacgccc gcgaaaaagt ccactttcag
     7261 ggcctccccg cgcgcatctg ctggctcggg gccggcgagc gtcacaaggc gggcctccgc
     7321 ttcaatgagc tggtggccga cggcgccatc
//
LOCUS       DATAIR010000836         4615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_183771, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000836 DATAIR010000000
VERSION     DATAIR010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183771"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VHM69_19915"
     CDS             complement(<1..390)
                     /locus_tag="VHM69_19915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2742717.1"
                     /translation="MKSTAHGERLVQLTRYPKVFPVNVYLVREEDGFTLVDTGLSGSE
                     EAYLAVARELGGEIRRIALTHAHGDHTGSLDALREALPEAEVLVPVREASFLRGDLDL
                     TPEEQRLGELKGWWKDARTEPTREISAG"
     gene            complement(598..810)
                     /locus_tag="VHM69_19920"
     CDS             complement(598..810)
                     /locus_tag="VHM69_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742718.1"
                     /translation="MSLEGVLVATTGTNRLSVVALGSAGGAYLAARLVSSTRAKVWVY
                     SFLVAVVVLSIICLLMVDSTQFLQHS"
     gene            991..1209
                     /locus_tag="VHM69_19925"
     CDS             991..1209
                     /locus_tag="VHM69_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742719.1"
                     /translation="MAEKSSEVLMVGARPLNLAEMVCRRLVEGVRILDDALLLDADPA
                     WAGAINTVLVKKGVSVSELRRAEDRAAV"
     gene            1349..1942
                     /locus_tag="VHM69_19930"
     CDS             1349..1942
                     /locus_tag="VHM69_19930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2742720.1"
                     /translation="MPAEENKLVVQRFWEEVVNEGNLEAVHEIFASNFVFHAPTLREG
                     EVSGPEAVSSMVEQLRATVLDLHFTIKDQDAIGNREVMTYLSATGIASGYVADEPDIE
                     PTGATLQWFGVSGSRFSSEEPGKIEGTWLGMVVRASDDTTGQNGTDGGTWSSGELATF
                     TERVEPGEERLDPEEELYRLQAGKTLRCRICFCLCCN"
     gene            2151..4304
                     /locus_tag="VHM69_19935"
     CDS             2151..4304
                     /locus_tag="VHM69_19935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2742721.1"
                     /translation="MSPAKLRGRLLFAARTVWISVALLAIGLFVAGVPVSYDHYVKVC
                     TGSGCGDWPLDPEDLSALEGLGLSAGFYATYNVALDVVHTLGFWALAAFIFWRRSDDR
                     VTLLISLMLVSFGTIGVDNNALYLLQSDHRTWWLLSRLIDSIALITFFASFYLFPDGR
                     FVPRWTRVLVVVFIVYQLSYSFLFPLFPMFEMRALSNLYSLLLLCLLSTLIFAQIYRY
                     SRVSGPVERQQTKWVVFGLTAAIAILVGANLIGMAFVEFRQPGLLGTLYTLGSRTLVN
                     LSFLFIPLSIGVAILRYRLWDIDLIINRTLVYVALTVSIIVFYGLVVSGLGALFRTDD
                     DFVISLIATGLVAVMFAPLRYRLQRNVNRLMYGERDDPYDVLSRLGRRLETTLAPEAA
                     LETVAEVVAQALKLPYAAIELRQGDSYKKVAEYGDLNGENTVLPLVHQGETVGRLVVA
                     PRSAGEEFSRQDRSLLEDLAWQAGAAAHAALLTTDLRRSRERLVTAREEERRRLRRDL
                     HDGLGPTLGGLTLGLDAARSTLAGDPGTTDELLAELKAQTREAVSDVRRLVHDLRPPA
                     LDDLGLVSAIRQQAAKHGHLQGDTVAGMQGASPMNGLAFTVDAPIDLPTLPAAVEVAC
                     YRIGQEAITNVSRHARASTCSIRISVEENRSELVLEVADDGVGMPESRRAGVGMSSMR
                     ERAEELGGTLRIESPAKEGGTRVLAWLPLPEEEER"
     gene            4301..>4615
                     /locus_tag="VHM69_19940"
     CDS             4301..>4615
                     /locus_tag="VHM69_19940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2742722.1"
                     /translation="MTSPEVTPAGDTRVLVVDDHAVFRYGMRAMLANAKGFEVVGEAA
                     TGEEAVEKAAEMRPDIVLMDIQMPGMNGIEATRRILQANPRVRVVVVTMFGDDESVFL
                     AMR"
BASE COUNT          907 a         1308 c         1443 g          957 t
ORIGIN      
        1 accggcagag atctccctgg ttggctctgt cctggcatct ttccaccagc ccttgagctc
       61 ccccaaccgt tgctcttctg gtgtgagatc caggtctccc cgcaggaagc tggcctctcg
      121 cacaggaacc aacacttccg cctcaggcag ggcctcgcgc agggcgtcaa gggacccggt
      181 gtggtcgccg tgagcgtggg tcagcgcgat gcgccggatc tcacctccca gctcccgcgc
      241 taccgccagg tacgcctcct cactccccga gaggcccgtg tccaccagcg taaacccgtc
      301 ctcctcacgc accagataga cgtttaccgg aaatactttg gggtagcgcg tcagttgtac
      361 cagacgctct ccatgagccg tcgacttcat cttctgctcc ttctcgcaga acaacgccgc
      421 atcacgttac taatatgatt agattataga ctaagatcat tagttagtca agataagaat
      481 ggggacggtt acagatctac tcgaatcggt tgaaccatcg ggatcggggc cgcgcgtgcc
      541 cggcactttg actgaaggaa gtagatcctg aagccgggtc tgcgaaagca gaccgcacta
      601 cgaatgttgc aggaattggg tgctatcgac catcagcagg caaataatgg acaggacgac
      661 aacggcgaca aggaagctgt acacccacac ctttgcgcgg gtgctggaga ccagccgtgc
      721 ggcgaggtat gcgccgccgg cgctaccgag ggcgacgacg ctgagtcggt tcgtgccggt
      781 ggtggcgacg aggacccctt caagcgacat cccgaactcc acttacgttt gccgagggca
      841 ttctacccac gttcgcggcg aaggcaccag gttgctctct acagaccttc ccgaatcaat
      901 cgggacactg ctcccatgac gccgggacac cttgcgagcc aacatacctt gtgaagcagg
      961 aggaacctga gagcgaggag gtgaacagcg gtggccgaga agagtagcga agttctgatg
     1021 gtgggcgcca ggccactgaa ccttgccgag atggtttgca ggaggctcgt tgagggggta
     1081 cggatcttgg acgatgcgtt actcctcgac gcagatcccg cctgggccgg ggcgatcaac
     1141 accgtgttgg tcaagaaggg tgtgagcgtg agcgagttgc gtcgagcgga agatcgagca
     1201 gcggtttgat ctctggcgca acagatcagt ttgtcgcaaa ttcatccgcc attcatcggc
     1261 gtctggcttc atgagcatca tggacatgca agtgtcggcc catcgaggcg ggtagaccta
     1321 gaggaggtgc cagatgtcgg aaggccacat gccggcggaa gagaacaagc tcgtcgtcca
     1381 gcgattttgg gaggaggttg tcaacgaagg aaacctggaa gcagtgcacg agatcttcgc
     1441 ttccaacttc gtcttccatg ctcccactct tcgtgaaggc gaggtcagcg gccccgaagc
     1501 tgtcagcagc atggtcgaac agcttcgcgc caccgtcctt gatcttcatt tcactatcaa
     1561 ggatcaggac gctatcggga atcgcgaggt gatgacatac ttgagcgcta caggcatcgc
     1621 aagtggatat gttgcagacg aaccagatat agaacctact ggcgccacgc tgcaatggtt
     1681 cggggtgagt ggctcccgtt tctcctccga agagcctggc aagatcgagg gaacttggtt
     1741 gggaatggtc gtccgcgctt ccgatgacac gaccgggcaa aatggtacgg atgggggaac
     1801 ttggtcttcg ggggaactgg cgactttcac ggaaagagta gagcctggag aagagcgact
     1861 ggatccggag gaagaactat ataggctcca ggcaggcaag accttacggt gccgcatttg
     1921 cttttgcctg tgctgcaact aggcttgtcg tcaagtagat cagaggacgt atgccacgat
     1981 tcgcattcgc ggatgagcag aggcacgccc cttccagcgc ttgccgcggg ctcatgagac
     2041 gttggtatcg taggctaacg cgagacacct cccggattcc tctgcggtca gcgcaaagga
     2101 gggtatctcg ctatcatccc aaaagaggga cagcgcaggt atctcaaacc ttgtcgcctg
     2161 ccaagctgcg gggccgtctg ctcttcgccg cccggacggt gtggatctcg gtggcccttt
     2221 tggcgatagg gctctttgtg gccggcgtcc cggtctccta cgaccactac gtcaaagtct
     2281 gcacgggctc tggctgcggt gactggccac tcgaccccga agatcttagc gcccttgagg
     2341 ggcttggtct ttcggctggc ttctacgcca cctacaacgt cgcgctcgat gtcgtgcata
     2401 ccttgggctt ttgggcgctc gcagcgttta tcttctggag aaggtcggac gatcgcgtca
     2461 ctctgctcat ctccctcatg ttggtgtcgt ttgggacaat cggggtagat aacaacgctc
     2521 tctacttact gcaatccgat catcgcacgt ggtggttgct gagcaggctt atcgatagta
     2581 tcgctctcat aactttcttc gcgagcttct atctcttccc tgatgggcgt ttcgtgcccc
     2641 gctggacccg cgtgcttgta gttgtcttta tcgtatatca gttgtcctat tctttcctat
     2701 ttccgctctt tcctatgttc gaaatgcgcg cgttgtccaa tctttacagt ttgttgctgt
     2761 tgtgcctcct cagtaccttg atattcgccc agatctatcg gtattcacgc gtgtccggac
     2821 ctgtagagcg ccagcagacc aagtgggtcg tcttcggctt gacggcggcg atcgctatac
     2881 tcgttggggc taacttgatc gggatggcct tcgtggagtt cagacagcct ggtctcttgg
     2941 ggacgctata cacgctgggt agcaggaccc ttgtcaacct gtcgtttcta ttcatacctc
     3001 tgtctatcgg cgttgcgatc ttgcgctacc gcttgtggga tatagacctg ataatcaacc
     3061 gaaccctcgt ttacgtggcg ctcactgtaa gcattatcgt gttctacgga ctggtggtga
     3121 gtgggctcgg cgcgctcttt cggacggacg atgacttcgt catctcgctg atcgcaacag
     3181 gcttggtcgc agtaatgttc gcgcctttgc gataccgcct ccagcgcaac gttaaccgcc
     3241 tgatgtacgg agagcgagac gatccctacg atgtgctctc gcggctagga cgccgcctcg
     3301 agaccacact ggcccccgag gcggcgctcg agacggtcgc cgaggtagtg gcccaggcgc
     3361 tcaaactgcc ctacgcggcc atcgaactca ggcagggcga cagctacaag aaagtggccg
     3421 agtacggtga tctaaatggc gagaacaccg tactcccgct cgtgcaccag ggcgagacgg
     3481 tggggaggct ggtcgtggcg ccacgctctg ctggggagga gttctccagg caggacagaa
     3541 gcctgcttga ggacctcgcg tggcaggctg gggcggcagc tcacgccgcg ctgctcacta
     3601 ccgacctgcg gcgctcgcgg gagcggctgg tcacagcaag ggaggaggag cgcaggcgcc
     3661 taaggcgcga cctgcacgat gggctgggac cgactttggg aggtctcacc ctcgggctgg
     3721 acgcagcacg cagcacgctc gctggcgacc caggcacgac ggatgagcta cttgcggagc
     3781 tcaaggcaca gactagggag gccgtctcag acgtgaggcg tctggtgcac gatctcagac
     3841 cgcccgccct cgacgacctt ggccttgtct ccgccatacg ccagcaggct gccaagcacg
     3901 gtcacctcca aggagatact gtcgcgggca tgcaaggggc cagtcccatg aacgggctgg
     3961 ccttcacggt ggatgctcca attgacttgc caacactacc cgccgccgtg gaggtggcgt
     4021 gctaccgcat tggccaggag gctatcacca acgtatcccg ccatgccaga gcgagcacct
     4081 gctcgataag gatctcggtg gaggagaacc gtagcgaact ggtgctagag gttgccgacg
     4141 acggggttgg catgccggag agtcggcgcg ccggagtggg gatgagctcg atgcgcgagc
     4201 gggcagagga gttgggtggg acgctgagga tcgagagccc tgctaaggag ggcggcacac
     4261 gcgtccttgc gtggctgccg ctgcccgaag aggaggagcg gtgacgtcac cggaagttac
     4321 tccggcgggc gacaccaggg tcttggtggt tgacgaccac gcggtgttcc gctacgggat
     4381 gcgtgccatg ctcgccaatg ccaaaggctt tgaggtggtc ggggaggcgg cgaccgggga
     4441 ggaggcggtc gagaaagctg ccgagatgcg tcccgatata gttctcatgg acatacagat
     4501 gcctgggatg aacggtatag aagccacacg taggatcttg caggcgaacc cccgtgtgag
     4561 ggtggtggtg gtgacgatgt tcggggacga cgaatcggtg ttcctggcaa tgcgc
//
LOCUS       DATAIR010000837         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_86830, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000837 DATAIR010000000
VERSION     DATAIR010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86830"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VHM69_19945"
     CDS             <1..1443
                     /locus_tag="VHM69_19945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2742723.1"
                     /translation="RDYAETVRASGDALLALLDDILDFSKIEAGEVRIETIDFDLREL
                     VDEAAAVFAERARDKGLDLVRSVEPDVPTALRGDPYRIRQVLTNLLSNAVKFTEEGRV
                     VLGCERGRDLEDKVTLRFEVSDTGIGITPEQQRRLFRSFTQADTSTTRRYGGTGLGLA
                     ISKQLVQLMGGEIGVESEPGIGSTFSFTLPLSKQPRRARVRRAEIKPAPLPANLSPVE
                     AKKRSARVLVAEDTLTNRMVAVELLKRRGYEVDVVSNGAEAVEAFSSTGYAAILMDIQ
                     MPEMDGYEATAQIRQLEGSLRHTPIIAMTAHALQSDREKAFSAGMDDYLSKPVRPEQL
                     DGVLERWIPQAPKQGRSQSRITDGGPTPDDSLDQTVLADLRTIQQEGGGNIVEMLIET
                     FLDETPTHVAALREAAAQGEAPLFKRTAHALNGICRGVGASRMASICQEMERLAGLGD
                     VTSAPDMLDRLGEEFARVKTLLATELSSTN"
     gene            1588..1941
                     /locus_tag="VHM69_19950"
     CDS             1588..1941
                     /locus_tag="VHM69_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742724.1"
                     /translation="MDAYDFDVRLTPAAREILRPGEALVLDWHRMAICCAGAGEMSLY
                     AANEVGLRKRKGLVRLKGEAPIYAARAIFPHLAGREIRLDARKTLGLRRFSSDLPGDF
                     GLRAVMGYLPERTAR"
     gene            complement(1943..2143)
                     /locus_tag="VHM69_19955"
     CDS             complement(1943..2143)
                     /locus_tag="VHM69_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742725.1"
                     /translation="MRGGASPASDDFFGFDSFECAIHSLAPSCEYCNVRVIGHGIEAD
                     DSFYCCAHCASMAGVEGVSDRV"
     gene            2176..2556
                     /locus_tag="VHM69_19960"
     CDS             2176..2556
                     /locus_tag="VHM69_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742726.1"
                     /translation="MGKLHPSEQITAPTDHVYKGSSMPDPEKVDLEGLRVHIGKMQTA
                     ALKAAKIIRGGEVHDGRQPESVSSAFAAAVKYAKKRAAEGAAPEEIKCEVNARMDRGP
                     LGRLPDITIDQVHEMIHTPFGENI"
     gene            complement(3190..4086)
                     /locus_tag="VHM69_19965"
     CDS             complement(3190..4086)
                     /locus_tag="VHM69_19965"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX2742727.1"
                     /translation="MDVLRSAVERFGRRAPVGLAADGEIPKAEKKLRGIEQETGKRPE
                     GRLPDFLLLGAQKCGTGYLFTRLSRHPDVERPVIKEVDFFSKHFGRGVGWYRSCFPAR
                     ADGKPSWSWEASPNNLFYPHAARRAAEVVPAAKLIALLRNPVDRAYSHYHHQVRIGHE
                     TLPSFEAALEAEEERLRGKREEMLRDEGYLPLEFPRFSYRARGIYADQIEEWRRHFDD
                     EQLLVVKSEELYESPRETITGIVRFLGLPEWNPEDAYLSRAKTPRYSYEPMDPTTRKR
                     LEEYFEPHNRRLYGYLGRDFGW"
     gene            4321..4662
                     /locus_tag="VHM69_19970"
     CDS             4321..4662
                     /locus_tag="VHM69_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742728.1"
                     /translation="MGDDLLAGDEGRDIYLFGTPTDPGWGNDTISDSDPTPGGGVNVL
                     SFAGVDGNRNVTVTLSPGPEATDGTNAVEWVDAGDFRKVVGGSGDDVITGDEWPNIID
                     GGFGANTINGG"
BASE COUNT         1085 a         1585 c         1652 g          957 t
ORIGIN      
        1 cgcgactacg cggagaccgt gcgcgcttcg ggcgatgctt tactcgcgct gctcgacgac
       61 atccttgact tctccaaaat agaggcgggg gaggtcagga tagagactat agacttcgac
      121 ctgcgggagc tcgtggatga ggccgccgcc gtgttcgccg agcgggccag agacaaaggg
      181 ttagatctgg tgcgctccgt cgagcctgac gtgccgacgg ctctcagggg agatccttac
      241 cgcatcaggc aggtcctgac gaacctcctg agcaacgccg tgaagttcac cgaggagggt
      301 agagtcgtac tcggttgtga gcgtgggcgg gaccttgaag ataaggtaac gttgcgcttc
      361 gaagtctcgg ataccggtat cggcattacc cccgaacagc agaggcgcct gttccgttct
      421 tttactcagg ccgatacctc caccacccgc cgctacggag gcacaggtct gggattggcc
      481 ataagtaagc aactcgtaca actcatgggc ggagagatag gtgtcgagag cgagcccggt
      541 atcgggagca cattctcctt cacgcttccc ctgtcgaagc aaccgcggag agcgcgggtc
      601 cgacgagctg agatcaagcc ggcacccttg cccgcgaacc tgtcgcctgt ggaggcgaag
      661 aagcgctccg cgcgcgtcct cgttgcggag gataccttga cgaaccgtat ggtcgctgtg
      721 gagcttctga aaaggcgcgg ttacgaggtg gacgtcgtct ccaacggggc tgaagccgta
      781 gaggcgttct cgagcaccgg atacgcggcc atactgatgg atatccagat gcccgagatg
      841 gacggttacg aggcgacggc ccagatccgc cagctggaag ggtcgctgcg gcatacgcct
      901 atcatcgcga tgaccgccca tgccctgcag agcgacaggg agaaagcgtt ctccgcaggt
      961 atggatgatt acctctccaa accagtcagg cccgaacagc tcgacggcgt gctggaacgt
     1021 tggatcccgc aggctcccaa gcagggcaga tcccagagtc gaatcaccga cggcggtccg
     1081 acaccggacg actcgcttga ccagacggtc cttgcggact tgcgtacgat ccagcaagaa
     1141 ggcgggggca acatagtcga gatgctcata gagaccttcc tcgacgagac gccaacccac
     1201 gtggcagcat tgcgagaagc cgccgcgcag ggagaggctc cgctcttcaa gcggactgca
     1261 cacgccctga acggcatctg caggggggtc ggggcgagcc gcatggcctc gatctgccag
     1321 gagatggaga ggctcgccgg cctgggtgac gtgacgagcg ccccggatat gctcgaccgg
     1381 ctcggggaag agtttgcccg cgtgaagacc cttctcgcca ccgagctgtc atcgacgaat
     1441 tgagcccgtt tgcccatgcg agcagtaccg ctcacgtcac cgacctcaca taggatcgtc
     1501 aacttcggtt gcgagtgtgc ggcaggagat ccgaagctgc tgtgtgggtc ccgttggcat
     1561 cgtaagtcgc tatgctagac tcgtggcatg gacgcctacg acttcgacgt tcgcctgacg
     1621 ccggcggcgc gggagatact gcgcccgggc gaggcgctcg tcctcgactg gcacaggatg
     1681 gcgatctgtt gcgccggtgc cggagagatg agcctctacg ccgcgaacga agtgggattg
     1741 cgcaagcgca agggcctcgt ccgcctcaag ggggaggccc cgatctacgc cgcccgcgcc
     1801 atctttccac acctcgcggg acgcgagata agactcgacg cccgaaagac actcggcctc
     1861 cgacgcttct cctcggacct gcccggcgac ttcgggctgc gtgccgtaat gggctatctg
     1921 cctgagagaa cggcgcgtta gctcagaccc tgtcggatac gccttccacg cccgccatgc
     1981 tcgcgcagtg ggcgcagcag taaaatgagt cgtccgcctc gatgccgtgc ccgatcacac
     2041 ggacgttgca gtactcgcac gatggggcca gagagtggat cgcgcactcg aagctgtcga
     2101 agccgaagaa atcgtcggat gccggtgagg cgcctccccg catcgggcgc agacagtttg
     2161 tttatgtagc agttattggg gaagctgcac ccctcggagc agatcacggc accgacggac
     2221 catgtgtata agggcagctc gatgcctgac ccggagaaag tggatcttga aggcctccgg
     2281 gtacacatcg gcaagatgca gacggctgcg ctgaaggcgg cgaagatcat ccgcggcggc
     2341 gaggtccacg atggccgtca gccagaaagt gtatcgtcag ccttcgccgc ggccgtcaag
     2401 tacgcgaaga agagagcagc agaaggtgcg gcgcctgaag agatcaagtg cgaggtgaac
     2461 gcgcggatgg accgagggcc gctaggtcgg ctgcctgaca tcaccataga tcaagtgcac
     2521 gagatgatcc acactccgtt tggcgagaat atctaactga acagttagcc ccatgtgaag
     2581 ctccttcccg agatggaagg agttgctgac aacaaaagcc tcgaacagtg ggaccgtctg
     2641 gccctgctcc tgatcgcacg tccggtcgaa gacttcgagc cagagtttga ggagattact
     2701 ctccgcgaga tcgagcggct aaagcccctt ccagcatcga aagcgggcgt aaggcgacta
     2761 ccaaacatcc gccagtattc gaacgccgct accaactgca agaagctccc tgcgtgcaag
     2821 ccatcaatct gctgcaaagc aaaagaggcc gactccccga caagagcggc tccgacgacc
     2881 taaagaaagg tctgtggatg actctagcgc caaaaacgtt ataccccaga ggtaggacag
     2941 aaatatgggt cctcttcgac ttgcgggacg aatgaggctt cgagggtcga agacgaactc
     3001 tactatgcca tccgtcccat gctagccgtc tcaggtgggc ggcttatgat gctctccact
     3061 cccgcagggc tttccgccgg gtaatccggg cggggcggct ctcggttcgg atgctacggg
     3121 gcttcgtcgg gcactcttat gggccggcct ccccgccggc cacgattgcg gggttggtgg
     3181 tgaggccggc taccagccga aatccctgcc caggtacccg taaagccgcc ggttgtgagg
     3241 ctcgaaatac tcttcgagcc gcttccttgt ggtcgggtcc atgggttcgt aggagtacct
     3301 cggcgtcttt gctctcgaga gataggcgtc ttccgggttc cactccggca acccgagaaa
     3361 tcgcacgatc ccggttatcg tctcccgcgg actctcgtag agttcctcgc tcttgacgac
     3421 gagcagttgc tcgtcgtcaa aatgcctgcg ccactcttcg atctggtccg cgtatatgcc
     3481 ccttgcgcgg tacgagaagc gcgggaactc cagcggcagg tagccctcgt cgcggagcat
     3541 ctcttccctt ttgccccgca atcgctcctc ctcggcttcg agggccgcct cgaagctcgg
     3601 cagggtctcg tggcctatcc tgacctgatg gtggtagtgg gaataggccc tgtccaccgg
     3661 gttgcggagg agcgcgatca gcttggcggc gggaacgacc tccgccgcac gcctggccgc
     3721 atgcgggtag aagaggttgt tcgggctcgc ctcccacgac caactcggtt tcccgtccgc
     3781 gcgggcggga aagcacgaac ggtaccaacc cacgccccga ccgaaatgct tgctgaagaa
     3841 atccacttcc ttgatgacgg ggcgctcgac gtccgggtgg cggctcaagc gcgtgaacaa
     3901 gtagcccgtc ccgcacttct gcgcgccgag gagcaggaag tcgggcagcc ttccctcggg
     3961 ccgcttgccg gtctcctgct cgatcccacg cagttttttc tcggccttgg gtatctcccc
     4021 gtcggccgca aggcccacgg gggcgcgtct accgaacctc tcgaccgccg accgcagaac
     4081 atccaaggtg aacccccact cgcttgacta ccagcgcata gtaacatgcc ttttagccta
     4141 gcccagacgt ccctttttta ggcatcaaaa gtgtcaactt cggggccatt gttccgcatt
     4201 gtttaacaag ggccgcccca ccccgctaat tcgcataaca cagccacgag gatgggaaga
     4261 actattagaa ggtgggagtg gctcggacat catgaacggc ggccgctccg ggatttactt
     4321 ttgggcgacg acctgcttgc cggtgacgag gggagagata tttacctttt cgggacacct
     4381 actgaccctg gttggggcaa cgacaccata tcggactcgg accctacccc cggcggcgga
     4441 gttaacgtgc tctcgttcgc cggcgtcgat ggcaatagga atgtgactgt caccctgagc
     4501 cccggtcccg aagcgaccga tggcaccaat gccgtggagt gggtggacgc cggggatttc
     4561 aggaaggtcg tgggcggctc cggagacgat gtcataacgg gggatgagtg gccgaacata
     4621 atcgatggag gcttcggcgc gaacactata aacggagggt gaggcaatga cgtcctcgat
     4681 ggtgacgact acttggacaa cggacatgac ggtaccttgc gtggaggtaa cggtaacgac
     4741 cacatcagag cggtagcagt ttattccgga tcccaaagcg acgtgcaagg cggagccggt
     4801 gacgacaaca tcgaagcgca caacggtgtg ggatcatcga ctgcggagat ggcacagaca
     4861 ccgttacttt cgatgctggt ttggacacag tcaccaactg cgagtaaagc gccgaacctc
     4921 ggcgaactcc caatacgaag cccatagcgc cggtccaggc ggcccaggat cgccggcaag
     4981 gtagtagcgc ttcagtgggt acgcggcaaa gcgaaggccg ggcgcctgat atggagttgc
     5041 ggcggcctca tgagaaagtt cgggatgtgc ctcttccctt ttggtccatc ccagccttga
     5101 aggacacttt agatcaagca aaggggagag gcatcggccg cctggcacat ctttacaggg
     5161 cacgaaagag ccggagcccc gtaggacccc ggcctagcgc ttcgtgagat ggctgcagag
     5221 gttaactcgt agcctgctca ggcataggga caacgccgag ctgctgcata atgcccagc
//
LOCUS       DATAIR010000838         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_86911, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000838 DATAIR010000000
VERSION     DATAIR010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86911"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VHM69_19975"
     CDS             complement(<1..358)
                     /locus_tag="VHM69_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742729.1"
                     /translation="MFVRDARDAARRWVIEEASGAPDFNGAFYAGSTNWLPDDAVLPA
                     TSDVDVWVVLADPDPPEKPGKFIYRDVILEVSYLMDEQLGSPEEVLGDYHMAGAFRTP
                     GIISDPSGRLRGLQASV"
     gene            complement(391..1773)
                     /locus_tag="VHM69_19980"
     CDS             complement(391..1773)
                     /locus_tag="VHM69_19980"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX2742730.1"
                     /translation="MPETVTMDKIVAFCKRRGFVYQSSEIYGGIRSSYDYGPLGVEMK
                     RNIKEEWWRRMVHMREDMVGLDSAIIMHPKVWEASGHTSTFNDMLVESRTSKRRYRAD
                     HLIEAATGIDAEGLSPEELTKIIEEDERVKDPVDGGRDFAPVRPFNLMFETYMGPVKT
                     PENLAYLRPETAQGIFVNFKNVLQTSRVKVPFGIAQQGKSFRNEITPGNFIFRTREFE
                     QMEMEFFVKPGTDEEWHEYWIEERWNWYTDLGLKEENLRRYEHPKEKLSHYSKRTVDL
                     EYNYASVGWSELEGIANRTDFDLKQHAKFSGETLEYFDQATGERYVPYVIEPAVGPDR
                     IMLAFLVDAYTEEEVNGEERTVLKLHPRIAPTKAAVFPLSKKEPVSTIARELYDDLKG
                     DYRLFYDDSGSIGRRYRRQDEAGTPFCVTVDFDTIEDKKVTIRDRDTLEQERVPIAAV
                     RDRLKALISG"
     gene            1945..>2234
                     /locus_tag="VHM69_19985"
     CDS             1945..>2234
                     /locus_tag="VHM69_19985"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX2742731.1"
                     /translation="METLIWAGIGMGLASVAGVRAFVPLALYALFAQLGFIVPPDLLG
                     LQTGWNVVGVLGALAVLEIALDKVRALDPAFGYAMIPVRIAAGAALFETMYEP"
BASE COUNT          387 a          677 c          675 g          495 t
ORIGIN      
        1 acacggacgc ctggagtcct ctcaatctgc ctgatggatc cgagatgatg ccaggcgtcc
       61 tgaaagcgcc agccatgtgg tagtcaccaa gcacctcttc aggtgacccg agctgttcat
      121 ccatcaaata ggacacttcc agaataacgt cccgatatat gaacttccct ggcttctccg
      181 gtgggtcggg atcggcgagc actacccata catcaacgtc ggaagtcgcg ggaaggaccg
      241 cgtcatcggg gagccagttc gtcgaaccag cgtagaaggc tccattgaag tccggtgctc
      301 cagaggcttc ctcgatcacc caccggcggg ccgcgtctct ggcatccctg acaaacaccg
      361 tcgctgttgc agatcaccac gctgccgtga ttagcccgag atcagagcct tcagacggtc
      421 ccgcaccgcg gcaatgggta cacgctcctg ctccagggta tccctatcac ggatggtcac
      481 ttttttgtcc tcgatggtgt cgaagtccac cgtaacgcag aagggcgtgc ccgcctcgtc
      541 ctggcgcctg tagcggcgcc cgatcgagcc cgagtcgtcg tagaagaggc ggtagtcgcc
      601 cttgaggtcg tcgtagagct cgcgggctat ggtagagaca ggctctttct tggagagcgg
      661 gaagacggcg gccttcgtcg gggctatgcg gggatgtagt ttgagcaccg ttctttcctc
      721 gccgttcacc tcttcctccg tgtatgcgtc gacgaggaag gcgagcatga tcctgtctgg
      781 gcccacagca ggttctatga cgtaggggac gtagcgctcg cccgtggcct ggtcgaagta
      841 ctcaagggtc tcacccgaga acttcgcatg ctgcttcagg tcgaagtccg tacggttggc
      901 gattccttcg agctccgacc agccgacgga ggcgtagttg tactcgaggt ccacggtacg
      961 cttggagtag tgggagagct tctccttcgg atgctcgtag cggcggaggt tctcttcctt
     1021 caggcccaga tccgtgtacc agttccagcg ctcttcgatc cagtactcgt gccactcctc
     1081 gtccgtgccg ggcttgacga agaactccat ctccatctgc tcgaactcgc gggtacggaa
     1141 gatgaagttg ccgggtgtga tctcattcct gaaagacttg ccctgctgcg cgatcccgaa
     1201 aggcaccttt acccggctcg tctggagcac gttcttgaag ttaacgaaga tcccctgcgc
     1261 ggtctccggg cgcagatagg cgaggttctc aggggtcttg actggcccca tgtaagtctc
     1321 gaacatcagg ttgaagggac gcaccggggc gaaatccctt cctccgtcca cggggtcctt
     1381 gacccgctcg tcctcctcga tgatcttcgt cagttcttcg ggactcaggc cctcggcatc
     1441 tataccggtg gcggcctcga tcaggtggtc ggccctgtag cggcgcttgc tcgtgcggct
     1501 ctcgacgagc atgtcgttga aggtggacgt gtgaccggag gcctcccaca ccttcgggtg
     1561 catgatgatc gcggagtcta gtccgaccat gtcctcgcgc atgtggacca tgcgtcgcca
     1621 ccactcctcc ttgatgttcc gcttcatctc cacgcccaag ggtccgtagt cgtaggagga
     1681 gcggatgccg ccgtagatct ccgaactctg gtatacgaag ccgcggcgtt tgcagaaggc
     1741 cactatcttg tccatcgtca cggtctcagg cacggatcct ccgaactcac atagttacat
     1801 ttgagtccgg caagtatagc tcagagcggt ccgcggatta ggttgagtgc ctctgccatg
     1861 caagacgaag gatctcgcgc ccggtggcgg gaaggggcct gaggtcttcc ccggtgcgat
     1921 cctgcccgcg ttataatgtt ggtcgtggag actcttatct gggcaggcat aggcatgggt
     1981 ctcgcctccg tcgcgggggt gagggctttc gtgccgctcg cgctatatgc tctgttcgcc
     2041 cagctcggct tcatcgtacc gcccgatctg ctcggtctgc agaccggatg gaacgttgtc
     2101 ggggtacttg gggcgctggc tgttctggag atcgcactcg acaaggtcag ggctctcgac
     2161 ccggccttcg gctacgcgat gatcccggta aggatcgccg ctggcgctgc cctgttcgag
     2221 acgatgtacg agcc
//
LOCUS       DATAIR010000839         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_184225, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000839 DATAIR010000000
VERSION     DATAIR010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184225"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VHM69_19990"
     CDS             complement(<1..515)
                     /locus_tag="VHM69_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2742732.1"
                     /translation="MKRSKSADPAHDVLGYERQQLDAIFRPETVAVIGATERPGSVGR
                     TIMWNLVSSPFGGTVFPVNARRPNVLGIKAYPSVSEVPAKVDLAIIVAPAPTVPEIIR
                     ECVEADVEGAIIISAGFRETGPEGAALEREVLEEARRGRMRIIGPNCLGVMKPPTGLN
                     ATFAGAIARPG"
     gene            complement(736..1536)
                     /locus_tag="VHM69_19995"
     CDS             complement(736..1536)
                     /locus_tag="VHM69_19995"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2742733.1"
                     /translation="MPQATSDLARTLGEFVPHLVGAVVVLLVALVLAVLLQRLTTRFL
                     EGLGLDELFERTGAANSLRQLGYEGGPSRLIGAVLFWGIMLTGVAGSLSILGLSSLQT
                     TMDQIVNLSGRALVALVILIAGILSAGWLAELVAREAERAELRGSNTFRRIVFITVVV
                     IAALLAASQLGLETYILVLLAVVVLATIGLVTALALGQGLALLSGNIAAGRYVQDGTT
                     LGDVISVEGIEGTVEELGYASVTLRSEDGHLYRIPNRTLLENVVRKRS"
     gene            complement(1536..1886)
                     /locus_tag="VHM69_20000"
     CDS             complement(1536..1886)
                     /locus_tag="VHM69_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742734.1"
                     /translation="MMPVGEQRWEMLERIGAYAAGELEGDEAREVEQLIFERPDYRRL
                     AESYARMLVMLTTLGNEPVEAPEAVVGYAIRRAYVSAFLRGAEDFIGALGRSYLNAFV
                     YYLGLKREDYGGGI"
     gene            complement(1883..2479)
                     /locus_tag="VHM69_20005"
     CDS             complement(1883..2479)
                     /locus_tag="VHM69_20005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2742735.1"
                     /translation="MSSAPGGAPDQALALRASRGDVEAFSRLVQEHSGIVYRVSLRIV
                     GADGAQDASQEVWIRVWRNIKNFRGDSAFSTWLYRITVNTCLSARRKEARREAREYGG
                     EEMPFLPEPSGGDADPEAAALSSERREEIQAALVHVRAEHRAALVLRHMEGLPYAEIA
                     SVLDVPEGTAKGWVSRGRAAMLLALSEEDHGRAEGTSV"
     gene            2695..3033
                     /locus_tag="VHM69_20010"
     CDS             2695..3033
                     /locus_tag="VHM69_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2742736.1"
                     /translation="MYGIEVRESGVGLVVELWGEFDIFCIGELKSMLTYVSSSRRPTL
                     VDLSGIAFLDLQSARELAVRSLLYARLLTFRNPSPEVMATVAALGLEGWLNIPPDTDR
                     DEPQLFSNVT"
     gene            3204..3737
                     /locus_tag="VHM69_20015"
     CDS             3204..3737
                     /locus_tag="VHM69_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742737.1"
                     /translation="MGSRGSVFEGKTLFVAVVGVVAALLVWGLTAAPAFAQATAGDVN
                     IQYVDCSQVQAAVAEQYNSGDAIAADNATAGNNGSAANAEATAEIAQELGISQEQVNA
                     CLNGADGAPTDNGADDGADTTTAGKVNSSTDVIPQTKSASELPNTGGAPLPGAVAGFA
                     LVAAGILSVGLIVRRGR"
     gene            3750..4631
                     /locus_tag="VHM69_20020"
     CDS             3750..4631
                     /locus_tag="VHM69_20020"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2742738.1"
                     /translation="MRIRDLIRRSADDPLLKVGAGAMILALGFAVVVAVFAFLDEPER
                     AVAARSSNDAAVEPLVRSDPGVDPWVEKDIPPPKTEPAPKPEPAPTSEPTVDPQPVSV
                     SIPEKRPDPPPEPRPERQSRAPSVNEVDETDFPLPTHRQVEAAHHPRHYDLAPGAIMG
                     LTIESIGLHDVPVLNSDSSSALDQGVIHLPDTSLPWSETPERNVYLAGHRLGWPGTGS
                     HLVFYRLGALRNGDEITLRDRDGRRYKYRVIDTFLVTPDDTWVTGRVRGRDLLTLQTC
                     TPIPTFEKRLIVRAERV"
BASE COUNT          860 a         1546 c         1509 g          857 t
ORIGIN      
        1 tttccggggc gggcgatagc gcccgcgaag gtggcgttca ggcccgttgg cggcttcatg
       61 acgccgaggc aattgggacc gatgatcctc atcctgcccc gccttgcttc ctccaggacc
      121 tcccgctcca gcgcggcccc ctcaggcccc gtctccctga aaccggcgga gatgattatg
      181 gcgccctcaa catcagcctc gacgcactcg cggattatct caggcacagt cggcgcgggg
      241 gcgacgatga tcgcgaggtc caccttcgcc ggcacctctg agacggaagg ataggccttg
      301 atgccgagca cgttcggcct gcgcgcgttc acggggaaga ccgtcccgcc gaacgggctg
      361 ctcacgaggt tccacatgat cgttcgccca acgctcccag gccgctcggt cgccccgatc
      421 acagccaccg tctcgggccg gaagatggcg tccaactgct gccgctcgta cccaaggacg
      481 tcgtgtgccg ggtccgcgct cttggaccgc ttcattagtc gctccttcgt cgctcctggg
      541 tttcaggcat caggcttcag gtatcaggtt acagatcctc ttgctactgt aggtctcctg
      601 gatgggaaac tcgctttgcc gaggccgctc gactatatcc gtcgcgcaat tactttgtag
      661 caccgggccg atgtcagggt ggtgaacccg cccacgggcc acatcagcgg gaggttttag
      721 cctgaaacct gatgcctaac tccgctttct gacgacgttc tccaggaggg tacggttcgg
      781 gatgcggtag aggtggccgt cttcggagcg gagggtgacc gaggcgtagc ctagctcttc
      841 gacggtaccc tcgatgcctt ccacggagat cacatctcct aaagtggttc cgtcctggac
      901 gtagcgtcct gccgcgatgt tgccagagag gagggcgagg ccctggccga gggcgagggc
      961 cgtgacgagg ccgatggtgg cgagcacgac cacggcgagg aggaccagga tgtaggtctc
     1021 aaggccgagc tgggacgcgg cgagcagggc cgcgatgacg actacggtga tgaagacgat
     1081 gcggcggaag gtgttcgagc cgcgcagttc ggcgcgctct gcttcgcggg cgaccagctc
     1141 tgcgagccat cccgcgctca gtatgcctgc tatcaggatc acgagcgcaa cgagagcccg
     1201 accggagagg ttgacgatct ggtccatggt ggtctgaaga gaagagagcc cgaggatgct
     1261 gagggagccg gccacccctg tcagcatgat gccccagaaa aggacggcgc cgatgagacg
     1321 cgatggcccg ccttcgtagc ctagctgccg gagagagttc gcggcccccg tcctctcgaa
     1381 gagctcgtcg aggccgaggc cctcgaggaa acgggtcgtc agacgctgaa gcagcacggc
     1441 cagaacgagc gccacgagta gcaccacaac cgcgccaacg aggtgcggta cgaactcgcc
     1501 cagcgtcctc gccaggtcgc tagttgcttg tggcactaga tcccccctcc atagtcctcc
     1561 cgcttgagcc ccaaataata gacgaacgcg ttcaggtaag aacggccgag ggcaccgatg
     1621 aagtcctcgg cgccgcgcag gaaggccgat acgtaggccc ttcttatggc atagccgacc
     1681 acggcttcgg gcgcctcgac gggctcgttg cctaaggtgg tgagcatgac gagcatcctg
     1741 gcgtaggact cggccaggcg cctgtagtcg ggcctttcga agatgagttg ctcgacctcg
     1801 cgggcttcat cgccttcgag ctcgccggcg gcgtaggccc cgatcctctc cagcatctcc
     1861 cagcgttgtt cgccgacggg catcatactg aggtcccttc ggccctgccg tggtcctctt
     1921 cggagagagc taatagcatg gcggcccgtc cgcgtgagac ccaacccttg gccgttcctt
     1981 cggggacgtc tagtacgctg gctatctcag cgtaggggag cccctccatg tgacgcagca
     2041 ccagcgccgc cctgtgctcc gcccgtacgt gcacgagggc ggcctgtatc tcctcgcgcc
     2101 gctccgagct gagggcggcc gcttccgggt cggcgtcgcc gccggacggt tcaggcagga
     2161 aaggcatctc ctcgccgccg tactccctag cttcccgccg cgcctccttg cggcgtgcgg
     2221 agaggcaggt gttgaccgtg atcctgtaaa gccaggtact gaaggcgctg tctcctctga
     2281 agttcttgat atttctccag acccggatcc agacctcctg gcttgcgtcc tgggcgccgt
     2341 ctgctccgac tatgcggagc gacaccctgt atacgatccc cgagtgctcc tgtacgagcc
     2401 ggctgaacgc ctccacgtcg ccacgagaag cacgaagggc gagggcctga tccggcgccc
     2461 caccaggagc actactcatt agttagtgtc tttcgcgcgt cgctaagata cagatggtcc
     2521 tttcgagaac tcatatcgcg gtaagatagc agaccccatt cgtacgggac acgctgcccg
     2581 aagccgggcg tcagacggag tttttgggag ccccgtaacc acgctggggt gtgctgagtc
     2641 ctacttgtag cggtgtgacc ctaaccttcc gaaccgcggg ggaagcgaga ggctttgtac
     2701 gggatcgaag tacgcgaatc aggcgtggga ctcgttgtcg agttgtgggg agagttcgat
     2761 atcttttgca taggtgagct gaagagtatg ctgacctacg tctcgtcgag ccggaggccg
     2821 accctggtcg acctctcggg gatagccttc ctcgacctgc aatcagcccg cgagctcgcc
     2881 gtccgctcgc tgctctacgc acggctcctg acgttccgaa acccctctcc agaggtaatg
     2941 gccaccgtcg ctgccctcgg gctcgaaggc tggctcaaca tcccgccgga cacggaccgc
     3001 gacgagccgc agcttttctc caacgtcacc tgaatcgcgc ggcctgagac ccgaacgttt
     3061 ttcttgagga tttgccctcg gggccgtaga acagatagct caagcagtat atgatgcggc
     3121 atatatcgta acgttctggg atgcccgagg agagcttcgc cggctggact cagagcatga
     3181 caagagggga tggggtgagg ttcatgggaa gcaggggatc ggtcttcgaa ggcaagacgt
     3241 tgttcgtagc cgtcgtggga gtagttgcgg ccttgctggt gtggggcctg acggccgcgc
     3301 cggccttcgc ccaggccacc gcgggagacg tcaacatcca gtacgtagac tgctcgcagg
     3361 tccaggccgc ggtcgccgag cagtacaact caggcgacgc aattgctgct gacaatgcca
     3421 cggccggtaa caacggaagc gcggccaacg ccgaggcgac cgctgagatc gcccaggaac
     3481 tgggcatcag ccaggagcag gtcaacgcct gtctgaacgg cgccgacggc gctcccaccg
     3541 acaacggcgc tgatgacggc gctgacacca ctacggcggg taaagtaaat agctccacgg
     3601 acgtgatccc gcaaaccaag tcagccagcg agcttcccaa cacgggtggg gcgcctctgc
     3661 ctggcgcggt cgccggcttc gcgctggttg ccgcggggat tctgtcggta ggtctgatag
     3721 ttcggcgcgg ccggtagcgc accagtaggg tgcggattcg agatctcatc cgcagatcag
     3781 cggacgaccc gctcctgaag gtcggcgcgg gggcgatgat cctcgcgctc gggttcgccg
     3841 tcgtggttgc cgtcttcgcc ttcctcgacg agccggagcg ggcagtcgcg gcgagatcct
     3901 caaatgatgc agccgtcgag ccgctcgtac gctccgaccc cggcgttgac ccgtgggtcg
     3961 agaaggacat acctccgccc aagacggagc ccgcgcccaa accagaaccg gcccccacat
     4021 cagagccgac cgtcgacccg cagccggtgt ccgtctccat acccgagaag cggccggatc
     4081 caccgccgga gcctcgaccc gaacggcaaa gtcgagcgcc ctcagtcaat gaagtcgatg
     4141 agacggactt tccactaccc acgcacaggc aggtcgaggc ggcccaccat ccacgccact
     4201 acgacctggc gcccggggcg atcatgggcc tcaccatcga gtcgataggc ctccacgatg
     4261 tgcccgtcct caactccgac tcaagcagtg ctctggacca gggggtcatc caccttcccg
     4321 acacctccct gccgtggtcc gagaccccgg agaggaacgt ctacctcgcg ggacacaggc
     4381 tcggatggcc ggggacaggc agccacctcg tcttctaccg cctcggcgcg ttgcgtaacg
     4441 gggatgagat caccctgcgg gatagggacg gaaggcgcta caaatacagg gttatcgaca
     4501 ctttcctggt gacccccgac gatacctggg taacgggacg cgtgagggga cgtgaccttc
     4561 tcacgctcca gacctgtacc cccataccta ctttcgagaa gcgcctgatc gtgagggccg
     4621 agcgagtcta gctttcagca cggcgcttcg cgccttttcg gccctcgctt tcagccgtca
     4681 gctagtccgg ccccgctctg ccgctaacga gcaaccattg cctgcggaga tgtggttcga
     4741 ggtgcgtggg tagcgcttcg ggacgacttg ct
//
LOCUS       DATAIR010000840         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_184589, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000840 DATAIR010000000
VERSION     DATAIR010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184589"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..915)
                     /locus_tag="VHM69_20025"
     CDS             complement(88..915)
                     /locus_tag="VHM69_20025"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX2742739.1"
                     /translation="MRPVCFLSDFGLADDFVGTCKGVILRIAPGVSIVDLTHEVPGFA
                     VEAGAEILQHATRYMPADTVYLAIVDPGVGTERRAIALLAESGALLLGPDNGLLVDAA
                     ESLGGVSEAVALTDEHYHLHPVSNTFHGRDVFAPVAAYLAAGVEISSLGEVVDPASLA
                     RLNPASALTGDEEGFVTHIISIDRFGNARLSVTQEESGLEYGDALKVDIGDGEMSVRY
                     VETFGLAKAGELVLVPDSHWRLSLAINKGNAAHALGLKVGGKVRLILPTETQEDAGA"
     gene            complement(1839..2849)
                     /locus_tag="VHM69_20030"
     CDS             complement(1839..2849)
                     /locus_tag="VHM69_20030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2742740.1"
                     /translation="MAALEYSRRLGSINDDQFRSALARLGLGEFVRAEPVSGGLFGQN
                     VLVASTTGEYVLRGAPHYDWQFPAERFFARLLHERTLAPIPWPYFLDPCDDIFGWSYA
                     LMPRMPGLQLADSAAAEGLTSDERLEMARSMGENLALLHALSWPCPGQYDLATDTIAP
                     LEESWNEWVAAEARRWLTLARRRSDVTTDADAGWVEDLLRSSHDALGEPFEPRFVMHD
                     YGEHNVTFEQTDGLWHVSGVFDLMEAFFGDREMDLSRQTAIYLEGDPALARTFLRTYL
                     ELLPARPGFQERFPAYMLLDRLVVWEYAQRPQHEPQWDRGLTLREWAEPYTASRITRD
                     LT"
BASE COUNT          571 a          951 c          959 g          622 t
ORIGIN      
        1 gccgcgcgca gcatccggtt tctctcctcg ggcgagagtg gagcgggcgc ctgctccacc
       61 ggcggaccgg cggaagggtc gcgcatctca ggcaccggca tcctcttgag tttctgtcgg
      121 gagtatgaga cgcaccttcc ctccgacttt caacccgagc gcgtgggcgg cgttgccctt
      181 gtttattgcg aggctgagac gccagtgaga gtcggggacg agcaccagct cccccgcctt
      241 ggccaacccg aaggtctcga cgtatctaac ggacatctcg ccgtcaccta tgtctacctt
      301 cagggcgtca ccgtactcga gcccggactc ctcctgcgtg acggagagcc tggcgttgcc
      361 gaaacggtcg atggagatga tgtgggtcac gaaaccttcc tcgtcaccgg tgagggcgct
      421 cgccgggttg agccgcgcca gtgacgccgg gtccactact tctccgagac tcgagatctc
      481 gaccccggct gcgagatacg ccgctaccgg agcgaagacg tcgcggccat gaaaggtgtt
      541 tgagacggga tgcagatggt agtgctcgtc ggttagcgcg actgcctcgg agacaccacc
      601 aagagactca gccgcatcga cgagcagccc gttatcagga cccaaaagca acgcgccgct
      661 ctccgccagc aacgcgatcg ctcgccgctc cgtgccgact ccagggtcga ctatcgccag
      721 atataccgta tcggcgggca tgtagcgggt ggcatgctgc aggatctccg cccccgcctc
      781 caccgcaaag ccgggtacct cgtgcgtgag gtcaacgatc gacacgccgg gtgcaatcct
      841 caagatcaca cccttgcacg tgccgacgaa gtcgtcggcg agaccgaagt cggataggaa
      901 gcaaactggt ctcatgcctc tagtataaag cgcaccgatg cccattgtag agactcccag
      961 agacgatctg ccaggagacc gcgccgagcc cccccgacac tattgcgcac atctcgacct
     1021 ttaaccaacc taaccttcta gtaatagttt aatgtacgtt ttgtggcaga ttaacatttt
     1081 ggtttagact caacatattg tgccgtgtgg tcttgggcaa tgaacatatc gtatacgatt
     1141 gcacgtgcag catcgtttct ggcttaggta aagggattgt aaatagttgc ggtctgtgta
     1201 ataggatgct agcgttttgt tcaggcggtc ctcgcccacc caggggctcg gtttaaagct
     1261 tgggtacaga tgtggggatc gtgacggact tcatcggcgg ggaggggtga tggggtccgg
     1321 ctaaaggctc cttatgggga ggggccagtc atggcgaccc gtcggtgctt ccgggtcgcc
     1381 atttctcgtg tctatggcgg ttctcgaaaa ggatgagcta ctccttcgga gaaggcttca
     1441 ggcttcaggt ctttttgctg atgcctgtag cctgaaacct ttactcacct caggttcact
     1501 ctttccgcaa accgctctag cggggctctc tactcgactt ctatgccagg tcggtccaca
     1561 tgacggcgca tcgaaggagg ggcggaccgc cacttctagt ggatctaaca gcggcttgcg
     1621 gaaggattga tcctcccaca agccgggctt caggtttcag gcttcaggaa ggtcttcccg
     1681 gactgagggt ggtcgctaag gtgcgcctct ggggctaagt ggatcattac ttatctactg
     1741 cgctgtgaga aggcacttgc ggggtgcgac ctacccgggg tcgtccgcca gcactggtgc
     1801 ggcatgccgt ctgagtagcg gatctatcca atcgggtatt acgtcagatc ccgcgtgatg
     1861 cgcgatgcgg tgtacggttc cgcccactcg cgcagcgtca gcccgcggtc ccactgtggc
     1921 tcgtgttgcg ggcgctgggc gtactcccac accacgagcc ggtcgaggag catgtacgcc
     1981 gggaatcgct cctggaatcc gggacgggcg ggcaatagtt ccaggtacgt cctcaggaac
     2041 gtgcgcgcca gtgctggatc cccttccaga tagatggccg tctgacgcga gagatccatc
     2101 tctctatcgc cgaagaatgc ctccatcagg tcgaacacgc cgcttacatg ccagagcccg
     2161 tccgtctgtt cgaaggtgac gttgtgctcc ccgtagtcgt gcattacgaa gcgtggctcg
     2221 aagggctcgc cgagtgcgtc gtgagacgag cggagcaagt cctccaccca gcccgcgtca
     2281 gcatccgtcg tcacgtcgga gcggcgtcga gcgagcgtaa gccaccgccg cgcctcggcg
     2341 gcaacccact cattccagct ctcctccaac ggcgctatag tgtccgtggc gaggtcgtac
     2401 tgtcccgggc acggccagct cagcgcgtgc aacagcgcga ggttctcgcc catcgagcgg
     2461 gccatttcga gtctttcatc gctggtgagc ccctcggccg ccgcgctgtc cgcaagctgc
     2521 agccccggca tgcgcggcat gagcgcgtag ctccaaccga agatgtcgtc gcatgggtca
     2581 aggaagtacg gccacgggat cggtgccagg gtgcgctcgt gcaggagacg ggcgaagaac
     2641 cgctcggcag ggaactgcca gtcgtagtgc ggagcgcccc gcaatacgta ctcgccggtc
     2701 gtgctggcga ccaaaacatt ctggccgaag agaccgccgg aaacgggctc ggcgcgcacg
     2761 aattccccga ggccgaggcg tgccagcgcc gaccggaact gatcgtcgtt gatcgacccc
     2821 agtcgtcgag agtactccag tgccgccatc accgctcctt acggcttcga ccaggctcgc
     2881 acgggcgctc cttcaagtag cgccgtcagc ttaccgctac gtttcacgga gatccggtcg
     2941 cgttcgccct gaacctgtct tactcgaaga ttcctgcggc ggtgctggtg tacgcggtca
     3001 gccagtggag gtcctcttcg gtaaagggtt cagttgatgt gcgtgtcgcg tagacgacgc
     3061 cagtgacgcg ctcgtcgtcg aggatcggga ctgccaggga gtt
//
LOCUS       DATAIR010000841         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_88127, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000841 DATAIR010000000
VERSION     DATAIR010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88127"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1741)
                     /locus_tag="VHM69_20035"
     CDS             complement(107..1741)
                     /locus_tag="VHM69_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2742741.1"
                     /translation="MFRTEKPWLKVYESGNVEPETKIFEGSLYDLFRHAAEEHRGKTA
                     LSFYGTTFEFERLQALAEKMAASLAASGVSKGDRVALMLPNCPQYVISFFATVRLGAI
                     VTQINPMYVEREIEHILNDSGAQSIVVYADVYERVKSVLPDTDLKTVVVVDFKGEPQG
                     LDPGHSSFDAFLSADADPAPDVEIDPTEDVAALQYTGGTTGVSKGAMLTHRNLVANVL
                     QTIDVFVRNPDQFAGRACVGALPFFHIYGLTCVMLFGIRLGVNQILLPRFDVQEMLAV
                     FDKVRPTMFSGVPTMYMALLASGADLRKHHLHDVQIFNSGGSALPVNLKHSFEEKVGK
                     PLFEGYGLSEASPVTHNNPPFLGQGREGSIGIPIPSTDARIVDVETGEQEMPIGESGE
                     LIIKGPQVMKGYLNMPDETASTLKGGWLYTGDVAKMDEDGYFYIVDRKKDMIVASGYN
                     VYPREIEEVLFEHPDVAEAVAIGVPDEYRGESVKAFVVKKPGAAVTEDEILAFCKERL
                     AAYKAPKTVEFREELPKSTVGKLLRWVLADEERSSL"
     gene            complement(1855..>2100)
                     /locus_tag="VHM69_20040"
     CDS             complement(1855..>2100)
                     /locus_tag="VHM69_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742742.1"
                     /translation="HFEEIFNHKNLAACDEIMAEDFVEHAVAPFAQSAPGKRFVARQS
                     HWFRVADGKLVEHWATREDLPTMLQLGIIQPPGAPPY"
BASE COUNT          381 a          652 c          653 g          414 t
ORIGIN      
        1 gcggccccgg cgtcggagag ggcctcggac atgtagcggc caaggccgcg tccaccaccg
       61 gtgactacgg cggtcttgcc ttcgagcctg aagaggtcaa gggcgttcaa agcgagctcc
      121 tctcctcgtc ggcgaggacc cagcggagga gcttgccgac ggtgctcttg ggcagctcct
      181 cgcggaactc gaccgtcttc ggggccttgt aggcggcgag gcgctccttg cagaaggcca
      241 gaatctcgtc ctccgtcacc gcggcgccgg gtttcttcac gacgaaagcc ttcacggact
      301 cgcccctgta ttcgtcgggg acgccgatgg cgacggcttc ggccacgtcg gggtgctcga
      361 agaggacctc ttcgatctcc cgcgggtaga cgttgtatcc cgaggcaacg atcatgtcct
      421 tcttcctgtc cacgatgtag aagtagccgt cctcgtccat cttcgcgacg tctcccgtat
      481 agagccagcc gcctttcagc gtcgaggccg tctcatcggg catgttgagg tagcccttca
      541 taacctgcgg ccccttgatg atgagctccc cggattcgcc gatgggcatc tcctgctctc
      601 cggtctccac gtccactatc ctggcgtccg tcgaagggat gggtatgccg atgctgccct
      661 cgcgtccctg cccgaggaac ggggggttgt tgtgcgtcac gggagaggcc tcggagagac
      721 cgtagccctc gaacaggggc ttgccaacct tctcctcgaa ggaatgcttc aggttaacgg
      781 ggagggcgga gccacctgag ttgaagatct gcacgtcgtg caaatgatgc ttgcgcaggt
      841 cggcgccgct tgcgagtagc gccatgtaca tcgtcgggac gcccgagaac atcgtcggcc
      901 gcactttgtc gaagaccgcg agcatctcct gcacatcgaa gcggggaagc agtatctggt
      961 ttacgccgag cctgatgccg aagagcatca cgcaagtcag gccgtagatg tggaagaaag
     1021 gcagcgcccc gacgcaggcg cggcccgcga actggtcggg gttgcgcacg aacacgtcga
     1081 tcgtctgcag gacgttggcg acgaggttgc ggtgggtgag catagcgccc ttcgatactc
     1141 ctgtcgtacc gcccgtgtac tgtaacgcag caacgtcctc agtcgggtca atctccacat
     1201 caggcgctgg gtctgcgtcc gcggagagga acgcgtcgaa agagctgtgt ccagggtcca
     1261 gtccctgcgg ttcgcccttg aagtcgacga ctacaacggt cttcaggtcc gtatcaggca
     1321 acacggactt cacccgttcg tatacgtccg cgtagacgac gatggactgt gcgccggagt
     1381 cgttcaggat gtgttcgatc tcgcgctcga cgtacatggg gttgatctgg gtgactatag
     1441 ccccgagccg cacggtcgcg aagaaggaga tcacatactg cgggcagttc gggagcatga
     1501 gcgcgacccg atctcccttc gaaacaccgc tcgccgcgag cgaggcggcc atcttctcag
     1561 caagcgcctg caagcgctcg aactcgaagg tagtgccgta gaagctcagc gccgtcttcc
     1621 cacggtgctc ctctgccgca tgcctgaaca ggtcgtagag tgagccctcg aagattttcg
     1681 tttcgggctc gacgttcccg ctctcgtaga ccttgagcca gggtttctca gtgcggaaca
     1741 tatctcctcc tcgtcaaccg cctggcggtc cctttcccac gtatctaacc agaaatgata
     1801 ccggccaaac cccgtctgcg cctcctgagg agttatgaac acgggcgatg agatctagta
     1861 gggaggcgcg cccggaggtt gaatgatccc caactgcagc attgttggaa ggtcctcgcg
     1921 cgtagcccag tgctcgacca gcttgccgtc cgcgacacgg aaccagtggc tctggcgagc
     1981 cacgaagcgc ttcccgggag cggactgcgc gaagggcgcg actgcgtgct cgacgaaatc
     2041 ttcagccatg atctcgtcac aggccgccag gttcttgtgg ttgaagatct cctcgaagtg
//
LOCUS       DATAIR010000842         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_88165, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000842 DATAIR010000000
VERSION     DATAIR010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88165"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VHM69_20045"
     CDS             <1..1149
                     /locus_tag="VHM69_20045"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX2742743.1"
                     /translation="EGEADLASVVGEARKAAHDSVRRTPELLDVLREAGVVDAGGLGV
                     AVILDGLYACVTGQEIEVPEESDKGAPDLDAIHASEEAWGYCTEFLVDGFEGNAEEFQ
                     EHIYASGRSVLVVDDDDVVKVHLHTQDPGAALTYAGTFGRLAGVKVDDMEAQVRSRDS
                     EKRPAARLGVVAASRGEGNRALFEQMGAVVIEGGQGENPSAADLARAVEETGAPAVVL
                     LPNNKNIVPTAEQVGELVDVQTHVIPTTSIAAGVAVMVGYDAEGEPDEVVDEMREISD
                     SLRSAEVTRSVRDARIGDREVPEGAYMGFLSGELIAVEGRVEDAALVLARKVVNEGAD
                     LITLLQGEDLSEAELDKIVNDISGLDDAVEVEVRDGGQPLYPLQMVAE"
     gene            1146..>1849
                     /locus_tag="VHM69_20050"
     CDS             1146..>1849
                     /locus_tag="VHM69_20050"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2742744.1"
                     /translation="MTTAVVTDSTTSLAPGVLERPDVRMVPLTFHFGADETYTDKVDM
                     SNEEFYERLRDSDVFPTTAQPPVGAFVETYEALSDYDDILVLTLSSKVSGTFDSASQA
                     AEMVDRPVHVLDTRSAEMGSGLILLEALRCIDEGGDFEEVRHAAERAIGEANVLFAVG
                     TLEYLAKSGRIGRAQRLVGTALDVRPVLKLTGGEVVPFKRTRGHRRQMAAMVEEVRPA
                     VEAGRPLYFGHIAAPEL"
BASE COUNT          342 a          560 c          651 g          296 t
ORIGIN      
        1 gaaggagagg ctgacctggc gtccgtagtc ggggaagcga ggaaggcggc ccacgattcg
       61 gtgcgccgca cccccgaact actcgatgtc ctgcgcgagg ccggtgtcgt tgacgccggg
      121 ggtcttgggg tggccgtgat cctcgacggc ctctacgcct gcgttacagg gcaggagatc
      181 gaagttcccg aagagagcga taaaggagcg ccggacctcg atgcgatcca cgccagtgaa
      241 gaggcgtggg gctattgcac cgagttcctg gtagacggct tcgaaggcaa tgcggaagag
      301 tttcaggagc acatttacgc ctcgggcagg agtgtgctcg tggtcgacga cgacgacgtg
      361 gtcaaggtcc acctgcacac ccaggacccg ggcgcagcgc tcacttacgc gggcaccttc
      421 ggccgactgg caggcgtgaa ggtggatgac atggaggccc aggtccgctc ccgcgattcc
      481 gagaagaggc ccgccgccag acttggtgtc gtcgccgcga gccgcgggga gggcaaccgc
      541 gccctcttcg agcagatggg cgcagtcgtg atcgagggcg gccagggcga gaacccgagc
      601 gccgcggacc tcgccagggc tgtcgaagag accggggccc ccgcagtcgt tctcctgccc
      661 aacaacaaga acatcgttcc caccgccgag caggtcggcg agcttgttga cgtacagacg
      721 cacgtcatac cgacgacgag catcgcggcg ggcgtggctg taatggttgg ctacgacgca
      781 gagggcgagc ccgacgaggt cgtcgatgag atgcgtgaga tctccgactc gctgcgctct
      841 gctgaggtta ctcgctctgt gcgcgatgcc cgcatcggcg atcgggaggt gcccgaagga
      901 gcatacatgg ggttcctctc aggcgagctc atagccgtgg aggggcgagt cgaggacgcc
      961 gcgctcgtgc tcgcgaggaa ggtcgtgaac gagggggccg acctcatcac gctcctgcaa
     1021 ggcgaagacc tctctgaggc ggagctagat aagatcgtca atgacatcag cggcctcgac
     1081 gacgcggtcg aggtcgaggt cagggacggc gggcagccgc tctatccgct acagatggtg
     1141 gcagagtgac cacggccgta gttacagact cgaccacgag cctcgcgccc ggtgtgctcg
     1201 agcggcccga cgtaaggatg gtcccgctga ctttccactt cggggcagat gagacctaca
     1261 cggacaaggt agacatgtcc aacgaggagt tctacgagcg tctgcgcgac tccgacgtct
     1321 tcccgaccac ggcccagccc cctgtggggg cgttcgtcga gacctacgag gcgctctccg
     1381 actacgacga catcctggtg ctcaccctct ccagcaaggt cagcggcacc ttcgactccg
     1441 cgtcgcaggc ggcagagatg gtggaccgtc ccgtacacgt gctcgacacc aggagcgccg
     1501 agatggggtc gggtttgatc cttctcgaag ccctccgctg tattgacgag ggaggggatt
     1561 ttgaagaggt caggcatgcc gcggagaggg ctatcgggga ggccaacgtg ctcttcgccg
     1621 tgggcacgct cgagtacctg gcgaagagcg gacgcatcgg aagggcacag cgcctcgtcg
     1681 gtacggcgct tgacgtcagg cccgttttga agctgacggg cggtgaggtc gtgccattca
     1741 agcgtacccg cggacacagg cgccagatgg ctgccatggt cgaggaggtc aggccggcgg
     1801 tggaggcggg taggcccctc tacttcgggc acatcgccgc ccccgagct
//
LOCUS       DATAIR010000843         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_88555, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000843 DATAIR010000000
VERSION     DATAIR010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88555"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VHM69_20055"
     CDS             <1..1376
                     /locus_tag="VHM69_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2742745.1"
                     /translation="PEVSELQKELEACEEQLGSPGVAADLRRMQRVLERQERLLRRFT
                     ELGGPGFEGEARGYLMSLGLGDGDVERPMSDLSGGQRKLAVLASCLARRPDVLLLDEP
                     EAHLDAERRERLEAIVRAFDGAVVIASHDRYLLDETVEQIAELEDGRITSWPGNYSAY
                     TVARELKLKRQQQLYVSQQKEIARLEEAIKRFKLWASMVENERHIKQARNKQRQIDRM
                     DKVERPVLERRKIGLALRDRVRGGRNVVQLREASVAFDEDPVLIGVDLAVERGERLGI
                     VGPNGAGKSVLAKLLAGVLCPTEGERKAGPSIEIGYLAQNDEPPPGASPLGLVRDVRP
                     LYEDEAVKLLGRFLFRYEQVREPVTSLSGGERTRLQLLLLMLEEPNCLVLDEPTNHLD
                     IDSLEILEAELERFPGTVVFVSHDRYFLDRIADGIVEVRDGDVHRHEGGYSEWRRSTF
                     QHAAFRS"
     gene            1689..1991
                     /locus_tag="VHM69_20060"
     CDS             1689..1991
                     /locus_tag="VHM69_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742746.1"
                     /translation="MGIEKSFGAEVKVTLPHPEQRQLFMVVFSSLEVGPAAVIRAHGG
                     RIVLDGGAWIIAELAFNVATSLRNSTDVVFLGGISLDPERFAAFSRLTGLNPLNPQ"
     gene            2088..2609
                     /locus_tag="VHM69_20065"
     CDS             2088..2609
                     /locus_tag="VHM69_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742747.1"
                     /translation="MPSVGQELLDTPFPEMVAKLAMGIAQAQQALDENSVNTAVILAD
                     TEIDVVLNITQVIAADGTVSFQQSDPVSISLIQIGLIPTFYQFSEATIEVSMDIKTTT
                     SRETNVKVSASAKVGFAAWSASVTADVSHNRKFGKEVKGTSRLFTRLAPVPPPARIAP
                     VLTVVDNRPDGGV"
BASE COUNT          576 a          833 c          824 g          480 t
ORIGIN      
        1 ggccagaggt ctcggaactt cagaaggaac tggaagcgtg cgaggaacaa ctcggctcgc
       61 ccggggtggc cgccgacctg cgcaggatgc aaagggtcct cgaacgtcag gaacggctct
      121 tgcgccgctt cacggaactc ggtggaccag gcttcgaggg tgaggcacgc ggctacctga
      181 tgtctttggg tctcggcgat ggcgacgttg agcgtccgat gagtgacctg agcggggggc
      241 aacgcaaact ggccgtcctc gcctcctgtc tcgcgcggcg tcccgacgtg ctgctactcg
      301 acgagcccga agcccacctc gacgccgagc gcagggagcg gctcgaggcc atcgtgcgcg
      361 ctttcgatgg cgccgtcgtc atcgcttcac acgaccgcta cctgctggac gagacggtcg
      421 agcagatcgc cgagctggaa gacgggagga tcacgtcgtg gcccggcaac tactccgcct
      481 ataccgttgc gcgcgagctc aagctcaaac ggcagcagca actctacgtc tctcaacaga
      541 aagagatagc gcggctggag gaggcgatca aacgcttcaa gctctgggcg agcatggttg
      601 agaacgagcg ccacatcaag caggcccgca ataagcagcg ccagatcgac cgcatggaca
      661 aagtcgagcg tccggtgctg gagcggcgca agatagggct cgcgctccgc gacagggtca
      721 ggggtggccg gaacgtggtc caactccgcg aagcgagcgt cgccttcgac gaagaccccg
      781 tcctcatcgg ggttgacctg gccgtcgagc gcggcgaacg tcttgggata gtcggtccca
      841 acggggccgg gaagagcgtg ctcgcgaagc tcctcgccgg cgtcctctgc ccgacggagg
      901 gcgagcgcaa ggcaggccct tcgatagaga tcgggtacct cgcacagaac gacgaacctc
      961 caccgggcgc atcgcccctc ggactggtac gggacgttag gccactctac gaggatgagg
     1021 ctgtgaagct gctcggacgt ttcctgttcc gatacgagca ggtgcgggaa cccgtgacct
     1081 ccctgagcgg gggagagcgc accaggttgc aactgctcct gctgatgctc gaggaaccga
     1141 actgcctcgt cctcgacgag ccgacgaacc acctcgacat agactcgctg gagatcctcg
     1201 aagcggagct cgagcgtttc ccaggcaccg ttgtcttcgt ctcgcacgac cgctacttcc
     1261 tcgaccgcat cgccgacggt atagtcgagg tgcgcgacgg cgacgtgcac cgtcacgagg
     1321 gcggatacag cgaatggcgt cgctcaacgt ttcagcacgc cgcctttcgc agctagtctt
     1381 ccctattttc gatctcgtag ccatcagcga gcgaaagcct gccgccctga gccccgacgg
     1441 aacctttgcc tccaagagcc agccaatttg ggctattcaa ccaacactca tcgcaaattc
     1501 atccagaatt catcgcggtg cgctatcttt tcaggtcgcc cgacgcatca acgccgtcgc
     1561 aggcgggcgg agaggaggtc caatagacag agtgtcacag accatcaaga ggtcgtgagt
     1621 tctggttggg cggcccagat cgccgtttcg tgagttccta acgtcaacag agatcgacga
     1681 ggatggacat ggggatagag aagagtttcg gggccgaggt caaggtgacg ttaccccacc
     1741 cggagcagcg gcagctcttc atggtcgtct tctcttccct ggaggtcggc cccgccgccg
     1801 tgatacgagc ccatggaggc cgtatcgtcc tcgacggagg agcctggata atagccgaac
     1861 tcgccttcaa cgtggcgacg agcctgcgaa attccacgga cgtcgtcttc ctcggtggca
     1921 tctcactcga tcctgaacgg tttgccgcat tcagcaggct cacagggctc aacccactca
     1981 atcctcaata gaactcagta ggactcggct ttggaacgaa gtagaagtcc ctagaacaac
     2041 aaggtttccc agatgaacca catccttaca aagaggagta gacactcgtg ccaagcgtag
     2101 gacaggaact tcttgatacc ccgttcccag agatggtggc caagctcgcc atgggtatag
     2161 cccaggctca gcaagccctc gatgagaact cagtaaacac cgcggtcatc ctcgctgata
     2221 cagagataga tgtagtgctc aacatcaccc aggtgatagc cgctgacggc acggttagct
     2281 tccagcagtc tgatcccgtc tccatctccc tcatccagat aggtttgatc cccaccttct
     2341 accagttctc ggaagccacc atcgaggtat cgatggacat caagacgacg acgagcaggg
     2401 agaccaacgt caaggtgagt gcctccgcaa aggtaggatt cgcagcgtgg agcgcttctg
     2461 ttacggctga cgtgtcccac aaccgcaagt tcggcaagga agtgaaagga acgagccggc
     2521 tgttcacgcg acttgcgccg gtaccaccgc cagcgaggat agctccagtg ctcacggtgg
     2581 tggacaaccg tcctgacggt ggtgtgtagt ggcgtttgac ctgaatccga gagatcctct
     2641 ggagcctacc caggagctac ctgacgcccc gctcgccgag gtcatcaccc agttggccga
     2701 aggagtggcc gag
//
LOCUS       DATAIR010000844         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_89024, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000844 DATAIR010000000
VERSION     DATAIR010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89024"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VHM69_20070"
     CDS             complement(<1..492)
                     /locus_tag="VHM69_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742748.1"
                     /translation="MTPTRVFVVAPTPTLRAGLRSMLGSVEAADLLVVGEAGFSAGPV
                     PDPSLSEADVVLVADEELLDETAVSVSEDGTQALLLLSEDEGVAFRLRAFALRGWGVV
                     PPDAPSEELGAAVAAVAQGLAVLPKSLAERLLDEPAAVEELSEPLTARESEVLDLLGH
                     GLSN"
     gene            complement(489..1349)
                     /locus_tag="VHM69_20075"
     CDS             complement(489..1349)
                     /locus_tag="VHM69_20075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2742749.1"
                     /translation="MAEEHLSAALAGARMIEGAQRSVVQVRSGGRGIGAGVIWPGDGL
                     VLTNYHVVAGGHGNVKVALHDGRSLDADVVKSSRSLDLALLRVGRENDLPTALIGDSD
                     ALRVGELVYAIGHPWGRIGAVSAGIVGELRGPGSSYIRSDVALAPGNSGGPLLNARGE
                     VVAINAMIFGSMALSIPSNAAGAWVAGERRPRLGIGVLPVEIPASLRGEAGASGLVIA
                     AVESGGAADRAGLLVGDVLLAAAGDPLDESETLLEAIARAGEAVSLRVMRGGRILVME
                     VRLEEAGRAA"
     gene            complement(1361..2260)
                     /locus_tag="VHM69_20080"
     CDS             complement(1361..2260)
                     /locus_tag="VHM69_20080"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2742750.1"
                     /translation="MEHKDTLGALSDGMADAVESIAGSVVRVNGRRRRSGSGVVFAQD
                     SVLTASHVLEREEDLSVETADGRTLPARFAGRDHSTDLAVLSVDGLNVEPAQPAEGDA
                     RVGQISLAVGHSHGEGPRATLGVVSAHGGPVRTRRGPRLERFIQTDATPFPGLSGGPL
                     IDARGKVLGILVSGWGRGAAFAIPADIAWRTAATLQERGSVKRGYLGILSQPVRLPDG
                     ERLGLTQRGGLLVVGVEDGSPAGRGGLIVGDILATLDGQPVEDTDDLLVLLAGERVGR
                     PVPVKLVRGGELTEVQITVGERG"
     gene            2448..4175
                     /gene="araD"
                     /locus_tag="VHM69_20085"
     CDS             2448..4175
                     /gene="araD"
                     /locus_tag="VHM69_20085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX2742751.1"
                     /translation="MSRKTRAEELRSHRWYGVRDLRSFGHRSRTKQMGYAAEDYSGKP
                     VIGIINTWSDINPCHAHFRERAEEVKRGVWQAGGFPVELPAMSLSEPFQKPSTMLYRN
                     FLAMETEELLRSYPIDGAVLMGGCDKTTPALLMGAITMDLPALFVPAGPMLRGNWRGR
                     TLGSGSDVWKYWDEKRAGTIGEHEWQEIEDGIARSPGHCMTMGTASTMTAVAEVLGMT
                     LPGASSIPAADSGHPRMASMTGRRIVEMVWEDLKPSNILNERSYEDAVTTVMALGGST
                     NAVIHLVALARRSGVLMPIDRFDEISRRVPVLANVRPSGEYLMEDFYYAGGLRGLLSR
                     LKEMLHLDRLTAGGGTLDETLDSAGVYNEEVIKPLDDPLQEEGGLAVLRGNLAPDGCV
                     IKHSAADPGLLRHTGPAVVFDGYDDLAGRIDDPNLEVDAGSVLVLRGAGPLGAPGMPE
                     WGLLPIPKKLLERGVRDMVRISDARMSGTAYGTCVLHVAPESHVGGPLALVREGDEIE
                     LDVPKRRLELKVDERELSRRRAAWKQPDLPYGRGYGAIFARHATQANEGCDFDFLAEG
                     PPVPEPEIH"
BASE COUNT          833 a         1595 c         1357 g          720 t
ORIGIN      
        1 gttggagagc ccgtgcccca gaagatctag gacctcgctt tcacgcgctg tcagcggctc
       61 cgagagctct tcgaccgcag cgggctcgtc gagcaaccgt tcggccaggg acttggggag
      121 tacggccagg ccctgcgcca cggccgccac cgcggccccg agttcctccg aaggggcgtc
      181 aggcggtacg acgccccaac ccctgagagc gaaggcgcgc agcctgaatg ccacaccttc
      241 gtcctcggag agcaagagta gcgcctgtgt gccgtcttct gagactgaga ccgcggtctc
      301 gtcgagcaac tcctcgtccg cgacgagtac tacgtcggcc tcggagagtg agggatcggg
      361 tacgggtcct gcggaaaaac cagcctcgcc gaccacgagc aggtcggcgg cctcgacgga
      421 gccgagcatc gaacgcaacc ctgcccgcaa ggtgggcgtc ggggccacga cgaacacccg
      481 cgtcggcgtc atgcggcgcg ccccgcctct tccagacgca cttccatgac caagattcta
      541 ccgccgcgca tcacccgcag agagacagct tcaccggcac gcgcgatggc ctccaataga
      601 gtttccgact catccagcgg gtccccggcg gcggcgagca gtacgtcacc gacgaggagg
      661 ccggcccggt ctgcggcacc gcctgattcg acggcggcga tcaccagccc cgaagctccg
      721 gcttccccgc gcaaagacgc cggaatctca acaggcaaaa ctccgatccc gagccgggga
      781 cggcgttcac ccgccaccca ggcacccgct gcgttgctcg gaatggagag cgccatactg
      841 ccgaagatca tcgcgttgat cgcgacgacc tcacccctgg cgttgaggag cggtcccccc
      901 gagtttccgg gtgcgagggc tacgtcagac cggatgtagg aggaaccagg tccacgcagc
      961 tcgcccacga tgccggcgct caccgccccg atcctgcccc acgggtggcc aatagcgtaa
     1021 accagttctc caacgcgcag tgcgtccgag tctccaatga gcgccgtagg caggtcgttc
     1081 tctctcccca cccggagcag cgcgaggtcc aggcttcggc tgctcttcac tacgtccgcg
     1141 tccagcgacc tgccgtcgtg gagggcgacc ttcacgttcc cgtgtcctcc ggcgaccacg
     1201 tgataattcg tcaggacgag accgtcaccg ggccagatca cacctgcccc gataccccgt
     1261 ccgccgctgc gtacctgcac cacacttcgc tgcgcgccct cgatcatccg cgcccccgca
     1321 agggcggcgg agagatgttc ttcggccatc tcccgatctc ctagccgcgt tcgccgacgg
     1381 ttatctggac ctccgtaagc tctccgccgc gcacgagctt gacaggcacg ggacgaccga
     1441 ctctctcacc agccagcaga acgagaaggt cgtcggtgtc ctccacgggc tggccgtcca
     1501 gcgtcgcgag gatatccccc actatgaggc cgccgcgccc ggcggggcta ccgtcctcga
     1561 cgccaacgac cagcaggccg cccctctgcg tgaggccgag ccgctctccg tcggggaggc
     1621 gcacaggctg gctaagaatg cctaaatatc cacgcttgac ggagccccgc tcctgaagtg
     1681 tcgccgcggt gcgccaggcg atatccgccg ggatcgcaaa cgccgcgccg cgtccccagc
     1741 cggagaccag gatgccaagc acctttccgc gcgcgtcgat gagcggcccg cccgagagtc
     1801 cagggaatgg cgtagcgtcg gtctgaataa agcgttccag cctcggaccc cgacgcgtgc
     1861 ggaccgggcc tccgtgagcg ctcacgaccc ccagggtcgc cctcggtccc tcgccgtgag
     1921 agtgacccac agccaaagag atctggccca cacgggcgtc tccctcagca ggctgcgcgg
     1981 gctcgacgtt caggccgtca acactcagca ccgcgaggtc cgtcgaatgg tccctgcctg
     2041 cgaagcgcgc tggaagcgtt cttccgtcgg ctgtctccac cgacaggtct tcctcgcgct
     2101 cgagcacgtg gctagccgtc aggaccgagt cctgcgcaaa tacgacgcca ctccccgagc
     2161 gacgcctcct tccgttcacg cgcaccaccg aaccggcgat actttcgacc gcgtccgcca
     2221 taccgtccga caatgcaccc aacgtgtcct tatgctccat gattctctcc tgatccggtt
     2281 aacgatacag acactctatc tccggcccta cggactggca tcacccgtcc ggctagttct
     2341 cgcccggaag aacggctagt aatccgtggc gactacccga tctcccggcg cattgcctga
     2401 tgacaccggg agtagaatgg gctatggaga gatatcgaag ggggaacatg agccggaaga
     2461 cgagggccga agagctgcgc agccaccgct ggtacggggt tcgtgacctg cgctcgttcg
     2521 gccaccgctc gcgcaccaag caaatgggct acgctgcgga ggactacagc gggaaacccg
     2581 tgatcggcat catcaacacc tggagcgaca tcaatccctg tcacgcccat ttcagggaga
     2641 gggccgagga ggtcaaacgc ggcgtttggc aggctggtgg attcccggtc gagctgccag
     2701 ccatgtcgct ctccgaaccg ttccagaaac catccacgat gctgtacagg aacttcctcg
     2761 caatggagac cgaggagctc ttgcgctcgt atcccataga cggcgctgtg ctgatgggcg
     2821 gctgtgacaa gaccacgcca gccctgctca tgggtgcgat caccatggac ctgccggccc
     2881 tcttcgtccc cgccggaccg atgttgcgcg gcaactggcg cgggcgcacg ctgggcagcg
     2941 gcagcgacgt atggaagtac tgggacgaga agcgcgcagg caccatcgga gagcacgagt
     3001 ggcaagagat cgaagacggc atcgcccgct cgccgggcca ctgcatgacc atgggtaccg
     3061 cctcgaccat gacagcagtc gccgaggtcc tcggcatgac cctccccggc gcctcttcca
     3121 tacctgccgc cgactccgga caccccagga tggcctcgat gaccggacgc aggatcgtag
     3181 agatggtctg ggaggacctc aaaccttcga atatcctgaa cgagcgctcc tacgaggatg
     3241 ccgtgacgac cgtgatggcc ctgggtggct cgaccaacgc cgtcatccat ctcgtcgccc
     3301 ttgcccgccg aagcggcgtc ctcatgccga tcgacagatt cgatgagatc tcacgtcgcg
     3361 tccccgtcct cgccaacgta cgccccagcg gcgagtatct gatggaggac ttctactacg
     3421 caggcgggct gcgaggcttg ctttcccgcc tcaaggagat gctacacctc gaccgcctca
     3481 ccgctggcgg tggcactctc gacgagacgc tggatagcgc cggtgtctac aacgaggaag
     3541 tcatcaaacc actcgacgac ccgctgcagg aagagggtgg actcgccgtg ttgcgcggca
     3601 atctggctcc cgacggatgt gtcatcaagc actccgccgc ggaccccgga ctactcaggc
     3661 atacaggacc ggcagtggtc ttcgacggct acgatgacct cgccggacgc atagacgacc
     3721 cgaaccttga ggtggacgcg ggctccgtgc tcgtcctcag gggggcgggc cccctcggtg
     3781 ctcccggcat gcctgagtgg ggcctgctcc ccatccccaa gaagctgctg gaacggggtg
     3841 tacgggacat ggtgcgcatc tccgacgccc gcatgagcgg caccgcgtac ggcacctgcg
     3901 tgctccacgt cgcccccgag tcgcacgtcg gcggcccact cgccctcgta agagaaggag
     3961 acgagatcga gctcgacgtc ccgaagcggc gccttgaact taaagtggac gaacgagaat
     4021 tgtcccgccg ccgcgcagcg tggaagcaac cagatctccc ctacggtcgc ggctacggag
     4081 cgatattcgc caggcatgca acccaggcca acgaaggttg cgacttcgat ttcctcgcgg
     4141 aaggaccgcc cgtgccagaa cccgagatcc actgaccttc aagccagcca acgacgtggc
     4201 acccctgatc cagcccacag gtagcgcccc gcaatggtat caagcgatgc tgtccgaggg
     4261 agaccctaca gcggcacggc cgtcaggcag cccgtcgctg actggtcatc cgtgactacg
     4321 agagcagttg ggcgcaatca gcccgcggcc tctatttcga tcgcgggtgc gacagcaaga
     4381 caaggtcgat ttcacccgac gtgctcgatc tccgctacct tgagcacgct catgcttcgg
     4441 ctttctctcc ttgacttgcg gcccgtcttg cagctgcagc cttagcccgg tcgtagttcc
     4501 ggccc
//
LOCUS       DATAIR010000845         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_89071, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000845 DATAIR010000000
VERSION     DATAIR010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89071"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VHM69_20090"
     CDS             <1..762
                     /locus_tag="VHM69_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742752.1"
                     /translation="GQTISEVGEGAGQAVQEAGEGVGQTTQEVGQGAGQAIQETGQGA
                     GQAVGEAGQGAGEAVGQAAEGVSDTAGQATEQVGQVAQEATDAVGEVTDTADQVAEGA
                     TDAVQGATEQATEAAGQATEQVQETAQDATQQATETAQGAVEGAQDAAGGVAQQAQDA
                     AGQATEQAAGDAEDAGQTTERTVDESGNILETVMNENGEVIGEDLVGNVGDLPVEEEY
                     IDDQGQVVSRAKDDAGNVYEQVFDDEGNLVEVRAA"
     gene            823..1689
                     /locus_tag="VHM69_20095"
     CDS             823..1689
                     /locus_tag="VHM69_20095"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2742753.1"
                     /translation="MAEARTNEALEKLNRGIEESKQMVQQQTMKLAQDFFADSVEETK
                     QEIKESRATLESLPDQIPGGQEESFQMLFQELMDNYEAIEQALDEAKKNVSELDIDRF
                     LKQGEIDATDAARRQAQELGLDLTEIEGTGSDGRITVDDVKNAAESAQEDVEDTVDEA
                     TEEVTKASDAAKRKAEELGVDLSQVEGTGSGGLVTVKDVTSAVGEVGQKATSTAGQAT
                     GQATDAVEQTAAQADGVVGQATDAAGGNEDGGPKATNAAKRKAEDLGVDLSQIEGSGA
                     GGLITIRDVVKN"
     gene            1686..1973
                     /locus_tag="VHM69_20100"
     CDS             1686..1973
                     /locus_tag="VHM69_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742754.1"
                     /translation="MKPAKKVRPDAIERPDVELTADVKARELRFDKVPETEIRFPGYS
                     DDETISGTVRENLPEEVEAGVTYRDARVRLRSAAQLPIPESGALRRKSGKE"
     gene            2049..3197
                     /locus_tag="VHM69_20105"
     CDS             2049..3197
                     /locus_tag="VHM69_20105"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2742755.1"
                     /translation="MTEGAQNLAQQIQEQAVNSAKDYYGKALGEVKGQLESSREELEA
                     LLEQLPEEQEEARAQIQELIDSYESIENALDEAAQTQGVEDTVNQALEQAQGAAQQVQ
                     DAAGDAAGQAQDAAGGAAEQAQDVAGGAAEQAQDAAGQAVGQVGEAAEGLAPGTQMLG
                     ESTDEEGNTVQRTVDESGDIIATTLDDAGNLVDEDIVGNVTDLPTAEGYEEETNEEGQ
                     TTRVVEDESGSLIHLQIGPDGNILDLEIPDLGDTVETVTDTVENTTEQVTGGGAGDAV
                     GQVADQATGAVGQVADQAGQAAQGAQDTAGQAAGQAQEAAGGAAQQAQDTAGQATEQA
                     QDAADQVDATEAAKQKADELGVDLSQIEGSGAGGRITVKDVVSAANQG"
     gene            3294..3605
                     /locus_tag="VHM69_20110"
     CDS             3294..3605
                     /locus_tag="VHM69_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742756.1"
                     /translation="MVQPNGAETGMQKEFKRQLVKAARMYAMCVKAEVAAPMDVVGIA
                     MAAFEDMPLKQALVLVRSNEQNVKDLAWALENSNSAQEFEQRVKEIKSLPHDHSDRES
                     G"
     gene            3634..3831
                     /locus_tag="VHM69_20115"
                     /pseudo
     CDS             3634..3831
                     /locus_tag="VHM69_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            4288..4848
                     /locus_tag="VHM69_20120"
     CDS             4288..4848
                     /locus_tag="VHM69_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742757.1"
                     /translation="MSLRDQERKAQQAERRMNEVMRRADREVGKKVEARAHEASLRAN
                     EVKRRIERGDVEPRPLPGLREDSMPSIASMPSMESSPSMKSIESADYARGVSDEAQQA
                     VEDGDVMMKDMPLEGTGMDPEAAHKDLAQRVTEETGSAGDPHQHAERKADEIAGQITE
                     DTGAEGDMQVHAGRKVEKMGQEMFED"
     gene            5119..5406
                     /locus_tag="VHM69_20125"
     CDS             5119..5406
                     /locus_tag="VHM69_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742758.1"
                     /translation="MFEARDAGRLASFEDRRMWRLLLARLHPDAGGDHDVFAFACAIR
                     DEMSGGNRTLRDTANEDSAEGQVAPFLRTWQEAMGQWSSSNRDAMNNFWKS"
     gene            complement(5474..5731)
                     /locus_tag="VHM69_20130"
     CDS             complement(5474..5731)
                     /locus_tag="VHM69_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742759.1"
                     /translation="MTQGNQQQQQGQQQQGQQQQQQVSPVQLLGRALTDIRKVQSFLE
                     LNNPDRGDEIKMQREAGDLIWNLIQSMQQQQQQGQQQQGQQ"
     gene            5950..6537
                     /locus_tag="VHM69_20135"
     CDS             5950..6537
                     /locus_tag="VHM69_20135"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX2742760.1"
                     /translation="MEGDRRTNGEEEYAAGTTDDRGESRGDPDVQLDVPKLNVEEIDL
                     EVEDLQVRVAFLAELADLVKINVGLDAEVREVKLQIKGVEAQAQLKARLDNVRAIFSE
                     VLSSLQHSPQFFREALGAANEATEMPPDTTWDTESADETVDTSAPADDGAAEPEATEA
                     ARKKARDLGIDLSGVDGTGSDGRILVRDVTKAAKG"
     gene            6585..6911
                     /locus_tag="VHM69_20140"
     CDS             6585..6911
                     /locus_tag="VHM69_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742761.1"
                     /translation="MANKFFKQTSAEILSDLLSDARVKRVREYVQGEEFKADRERIRR
                     DAAERLKDIRERYRGKRRDPETAARERELTLRLAEVEAESADLRIQLSELLEEEEKLR
                     SALDEL"
     gene            7199..>7552
                     /locus_tag="VHM69_20145"
     CDS             7199..>7552
                     /locus_tag="VHM69_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742762.1"
                     /translation="MTPGAGLYDPVYEHDACGLGFVARIDGRRTRETVEEGLEVLHNL
                     EHRGTTGSDPETGDGAGILTQIPDDFFRKECRELGIELPPPGSYGVGMLFESAEGLGC
                     EGRLAEICAEEGHDLL"
BASE COUNT         1752 a         2088 c         2625 g         1087 t
ORIGIN      
        1 ggccagacga ttagcgaggt aggagaaggt gccggacagg ccgtccagga ggccggcgag
       61 ggtgtcggcc agacaaccca ggaagtgggc cagggtgccg gacaggctat acaggagacc
      121 ggacaaggcg cgggacaggc cgtaggagag gccggtcagg gagcaggtga ggccgtagga
      181 caggccgccg aaggcgtgag cgacaccgcc gggcaggcta ctgagcaggt aggccaggtc
      241 gcgcaggagg ccaccgacgc ggtcggcgag gtgacggata cggccgatca ggtagccgaa
      301 ggcgcaactg atgctgtgca aggagcgacc gagcaggcga cggaggctgc ggggcaggct
      361 acagagcagg ttcaggaaac ggcccaggac gccacgcaac aggccacgga aaccgcgcaa
      421 ggggccgtgg aaggtgccca ggacgcagcc ggaggggtgg ctcagcaggc ccaggacgcc
      481 gctggtcagg cgacagagca ggccgcgggg gacgcggagg atgccgggca gacaaccgag
      541 cgtacggttg acgagtccgg caacatcctc gagaccgtca tgaacgagaa cggagaggtg
      601 atcggtgagg atctcgtcgg caacgtcggc gacctgccgg tagaggaaga atacatagac
      661 gatcaggggc aggtggtgag ccgggcgaaa gatgatgccg gcaacgtcta cgagcaggtc
      721 ttcgacgacg aggggaatct ggtcgaggtg agagccgctt gaggtagacg aagtcaggct
      781 gtttgccggg aagacatact aattagcagg gagaggagaa acgtggcaga agcacggacg
      841 aacgaggcgc tcgagaagct gaaccgcggc atcgaggaat cgaagcagat ggtccagcag
      901 cagacgatga agctcgccca ggactttttc gctgattccg tggaggagac caagcaggag
      961 ataaaggaga gccgcgccac cctggagagc ttgcctgacc agatcccggg cggccaggag
     1021 gagtcgttcc agatgctctt ccaggagctg atggacaact acgaggcgat cgagcaggcg
     1081 ctcgacgagg cgaagaagaa cgtctcggaa ctagacatcg accgcttttt gaagcaggga
     1141 gagatcgatg ccacggatgc ggcccgccgc caggcgcagg aactcggcct ggatctcacg
     1201 gagatagaag gcacgggctc ggacggccgt atcaccgtcg acgacgtgaa gaacgccgca
     1261 gaatccgcgc aagaagacgt cgaagatacg gtggacgagg cgaccgagga agtaaccaag
     1321 gcgtctgacg cagccaagcg caaggccgag gaactgggag tggatctctc ccaggtagaa
     1381 ggcacgggct cgggcgggct cgtaaccgtc aaggacgtca ccagtgcggt cggagaggtg
     1441 ggacagaagg cgacctcaac cgccgggcag gccacgggac aggcgacaga cgcggtcgag
     1501 cagactgctg cccaggctga cggggtggtg ggtcaggcca cagacgccgc cggagggaat
     1561 gaagatgggg gacccaaggc cacgaacgct gccaagcgca aggctgagga tctcggagtg
     1621 gatctctccc agatagaggg ctctggcgcc ggtgggctca taaccatccg cgacgtcgtc
     1681 aagaattgaa gcccgcgaag aaagtacggc cggacgcgat cgagagaccg gacgtagagc
     1741 tcaccgccga cgtgaaggct cgcgaactgc gcttcgacaa ggtccccgag acggagatac
     1801 gcttcccggg ctattccgat gacgagacca tctcagggac cgtgcgggag aatttgcctg
     1861 aggaggtaga ggcgggagtc acgtaccgcg acgctcgtgt ccggttgaga agcgcagccc
     1921 agctccccat acccgagtct ggtgcgctga ggcgtaaatc cggaaaggaa taggagcccc
     1981 cttgtagatg tgatgcaggt tgtaggcagc ggacgttaag tggaaagttc tctaggaaag
     2041 gatgaatagt gacagagggt gcacagaatc tcgcccagca gatccaggag caggccgtaa
     2101 acagcgcgaa ggactactac ggcaaggccc tgggcgaagt caagggccag ttggagagca
     2161 gccgcgaaga gctagaagcg ctgctcgagc agttgcccga ggagcaggag gaggcgcggg
     2221 cgcagatcca ggagctcata gactcctacg agagcatcga gaacgcgctt gatgaagccg
     2281 cccagaccca gggtgtcgaa gacacggtga accaggcgct cgagcaggcc cagggcgcgg
     2341 cccagcaggt acaagacgcg gctggagacg ccgcgggcca ggctcaggac gccgccggag
     2401 gagccgccga gcaggcgcaa gatgtggctg gtggagccgc cgagcaggct caggacgccg
     2461 ccggtcaggc tgtcggtcag gtcggagagg cagcggaggg cctcgcgccg ggaacccaga
     2521 tgctcggtga aagcaccgac gaagagggca ataccgtgca gcgcaccgtg gacgagtccg
     2581 gggacatcat cgcgaccacc ctcgacgatg caggcaacct cgtcgacgag gacatagttg
     2641 gaaacgtcac cgacctcccg acggccgagg ggtacgaaga ggagaccaac gaagagggtc
     2701 agacgacgcg cgtcgtagag gacgagtcgg ggagtcttat ccacctgcag atcgggccag
     2761 acggaaacat ccttgacctc gagatccccg accttggaga taccgtcgag acggttaccg
     2821 ataccgtcga gaacacgaca gagcaggtca caggcggcgg tgccggggac gccgttggac
     2881 aggtcgccga ccaggccaca ggagccgtgg gtcaggttgc ggaccaggcc ggacaggctg
     2941 cgcaaggcgc acaggacacc gccggtcagg ccgcgggcca ggctcaagaa gcagcgggtg
     3001 gagccgccca gcaagctcag gatacggccg gacaggctac cgagcaggct caggatgcgg
     3061 ccgatcaggt cgacgccacg gaggccgcca agcagaaggc cgacgagctg ggggtggatc
     3121 tctcccagat agagggttct ggtgctgggg gccgcatcac cgtcaaggac gtggtgagcg
     3181 cagcgaacca gggataaccg gcgcgggatg aggttcatgc ggcggggcca gagccccgcc
     3241 gcgctctagt tgcgctgacc atcgccgcag catctgaaac cgagaggagt agggtggtcc
     3301 aaccgaacgg cgcagagacc gggatgcaga aggaattcaa gaggcagtta gtgaaagcgg
     3361 ccaggatgta cgccatgtgc gtgaaggccg aggtcgccgc accgatggac gtggtcggca
     3421 tcgcgatggc ggccttcgag gacatgccgc tcaagcaggc gctggtactc gtgagatcga
     3481 acgaacagaa cgtcaaggac ctggcctggg cgctcgagaa ctccaattcg gcacaggagt
     3541 tcgagcaacg ggtcaaggag ataaagagcc tgccacacga ccatagcgac cgcgaatcag
     3601 gataggccgg aaggctccgt tggagactag aaggtgaaag atccctacgg gttattgggc
     3661 gtgtcgaggg gagcgtccga agaagacgta cgcaaagcct accggcggct ggcccgcgag
     3721 tatcatccgg acgcgaatcc cggcgacatc aagtccgagg agcgcttcaa ggaggtccag
     3781 caggcctacg agatcctctc ggaccccaac aagcgccgtg aatacgacga aagagcccgc
     3841 ccctcttcgc gcaagagacg ccccggatca tccagcaggg ctagcagagg gtcgaggaag
     3901 actaccactt cggcatcagg tggtcgagac ggtgggcggg agatcaactg gcaactcaac
     3961 gtcgagggag tcgaggacat agcccgcatc tccaggatcc tgggcatgga cattacccgc
     4021 ctctcgaagt tggcgggtga gggcataaag aggaagacga gttctgataa gagcggatcg
     4081 gccgacgcct cggacaagaa gccgcccaaa ccacggaaac cgcccaaacc gccgaaaacg
     4141 agtaaacgcc ccaatgctta gcagacccca gaacgaccgc gcggattgcc gggatggcga
     4201 gtcgatgcac gtcggaggcg caagctgact tcggggcacg ggagcggaac cgctttcgaa
     4261 atactcgcag attgaaaggg aagtcgcatg agcctacggg atcaagaacg gaaggcacag
     4321 caggccgagc gcaggatgaa cgaggtcatg cgcagggcgg atcgagaggt cgggaaaaag
     4381 gtcgaggcgc gggcacacga agcctccctc agggcgaacg aggtcaagag gagaatcgag
     4441 aggggcgacg tcgagccgcg cccactgcca ggtctcaggg aagactcgat gccatccatc
     4501 gcgtcgatgc cgtccatgga gtcttcgcca tccatgaaat ccatagagtc ggccgactat
     4561 gcacgagggg tgagcgacga ggcgcaacaa gcggtggagg acggggacgt catgatgaaa
     4621 gacatgccac tggaggggac aggtatggac cccgaggccg cgcacaagga cctcgcacag
     4681 agggtgaccg aggagacggg ttccgcggga gacccgcatc agcacgcaga acgcaaggcg
     4741 gacgagatag cgggacagat aaccgaggac actggcgctg aaggggacat gcaggtgcac
     4801 gcaggacgta aggttgagaa gatgggacaa gagatgttcg aggactgaag gcgtcaccat
     4861 tcgaagtacc ggtgagcttc caagaattgc tgtatcgaag ccctcaggga caagttcaag
     4921 tcctctctga caggtggcta ccttagagcg ccacgccgcc atcccgagca aggtggtctc
     4981 gctaaggttg aacgacccga gcccacatat ggctcggtat tcgtcctatt cctccaggta
     5041 ttgcggttgt gggataacga ggtttacaaa gtttcgcgga ccagcataga atgcgctcga
     5101 ctgaggaggt cctgtttagt gttcgaagcg agggacgcag ggcggctcgc atccttcgaa
     5161 gaccggagaa tgtggcgatt gctcctggcg cggttacatc ctgacgccgg gggggaccat
     5221 gacgtgttcg cgttcgcctg cgctataagg gacgaaatgt ctggcggaaa ccgcactcta
     5281 cgggacacag cgaacgagga cagcgcggaa ggacaggtgg ccccctttct tcggacgtgg
     5341 caggaggcga tgggccaatg gtcatccagc aaccgcgacg ctatgaacaa cttctggaag
     5401 agctaaaggg tgttgcggaa gtgatcttcc gcaacacgta ctttcaatct atattctgct
     5461 gattgctttt cggctattgt tggccctgct gttgttggcc ttgctgttgc tgctgttgca
     5521 tactctggat caggttccag atcaggtcgc cggcctcgcg ctgcatcttg atctcatcac
     5581 cccggtccgg gttgttgagc tcaaggaagc tctggacctt cctgatgtcg gtcaatgcac
     5641 gacccagcaa ctgcacggga ctcacctgct gctgttgttg ctggccttgc tgttgctggc
     5701 cttgctgttg ctgctgattg ccctgcgtca tcgagacctc ctgaccacac ggtccatttc
     5761 gacgtccatc ctatgcctgt gtataccccg tacgcgtcga agtaagcacg aaggtcaacg
     5821 tcgcgtcggc caaggcaagt ttacttacgg ggtatgaggg gcatgacaga caaatagcga
     5881 gacgtcttgt agtggcaagg gcttatgtga tccaggaggt ccgaagcgcg acgggaggag
     5941 cgtccagagg tggaaggcga caggcgcacg aacggggagg aagagtacgc cgccggcacc
     6001 acggatgacc gcggggagag ccggggagat cccgacgtac agctggacgt acccaagctc
     6061 aacgtcgagg agatcgacct cgaggtcgag gacctgcagg tacgggtggc attcctggcc
     6121 gagcttgcag acctggtgaa gatcaacgtc ggcctcgacg ccgaggtgcg cgaggtgaag
     6181 ctgcagatca aaggcgtcga agcccaggcc caactgaagg cccgcctgga caacgtgcgg
     6241 gccatcttca gcgaggtcct gtcctccttg caacacagcc cccaattctt ccgcgaggcg
     6301 ctgggagcgg cgaacgaggc cacagagatg ccgccagata cgacatggga cacggagtcc
     6361 gcggatgaga ccgtcgatac ctcggctccc gcggatgacg gggccgctga acctgaggcg
     6421 acggaagccg ccaggaagaa ggccagggac cttgggattg atctctcagg cgtcgatggc
     6481 acgggctctg atgggcgcat cctggtcagg gacgtaacga aggccgcgaa gggatagcct
     6541 tacctccagc gggtacaatc aggaggagtg gaaggagaat tcacgtggcc aacaagtttt
     6601 tcaaacagac atcggctgag atcctctcgg atctactctc cgacgcacgc gtgaagcgcg
     6661 tcagagagta cgtgcagggc gaggagttca aggccgaccg cgaacgtatc cggcgcgacg
     6721 ccgcggaacg cctgaaggac atccgcgaac gctaccgcgg caaacggcga gatccagaga
     6781 cagcagcccg ggagagggag ctcacgctgc gcctcgccga ggtagaggca gagtcagccg
     6841 acctgcgcat ccagctctcc gagctgctcg aggaagagga gaagctgcgc agcgcgctcg
     6901 acgaactgta gggcggcact tcgggacgct ccggcctcca ctctcaggta tcagcttttt
     6961 tcggtttcat ctgggattac cgctgacgac gcccaccacc gcatctcggt cgaaacgcag
     7021 gaccagcgcc tccctcgccg gctcgcgtca agatcctgaa agctgatgcc tgagccctca
     7081 ttttcccgaa gccttaccag atactagtat gcaacaggat tgctacgcac ttgtgctctg
     7141 ctggcaattc gtgtaaaaat aggcgctccg caccctggac gccataggag acgatttgtt
     7201 gacccctgga gccggacttt acgaccctgt gtacgagcac gatgcttgcg ggctcgggtt
     7261 cgtagcgagg atcgacgggc gcaggacgcg ggagaccgtc gaggagggtc tcgaggtcct
     7321 gcacaacctg gagcacaggg gcacgacggg gagcgacccg gagacggggg atggggctgg
     7381 catactcacc cagatccccg acgacttctt ccgcaaggag tgcagggaac tcggcatcga
     7441 gctacctccc cccggcagct acggggtggg catgcttttc gagtcggctg aggggctggg
     7501 ctgcgagggc aggctcgccg agatctgcgc ggaggagggc catgatctcc tt
//
LOCUS       DATAIR010000846         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_89387, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000846 DATAIR010000000
VERSION     DATAIR010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89387"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..>1639
                     /locus_tag="VHM69_20150"
     CDS             153..>1639
                     /locus_tag="VHM69_20150"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742763.1"
                     /translation="MILKDFSRILLEELDADCPLPGDASRQVRGLAVDEPVKAWISRD
                     EVAVTARDTLDEDFMEGVAATGSPAIVWRLDGEAQPEDVERAAALGLGLFVLTPDLPL
                     RRLFEIFSHDDGLLLLSHGAARALLESASGGSTVEDLAGRISGMLRRSVVIEDPVGRL
                     IASSPGKDRLLTTLEEGDIRASKSSGIDETRRERRDRYARLPDGYLSVPVERWRVADD
                     ELFWTPIGTGAPAGYLWIDLDRTNLKPEDVVLLYWARRVLEAELGKERIRLETELGVR
                     GDFVDDLVSGHYGSVELLLQRARYLGADLAEGALVMVVDIDDFARYLERRRPKEPAIQ
                     ELKRRLADAVRLQTRQLFSNFLLGPRSDNVILFLGSSEGEDPDELPEKAQLLAKGVQR
                     YVKGLLPDLTVSVGIGRYKKDPALLPDAYSEAGVALEIGHRIHGPSSVSTFEGTGTYK
                     LLFRVLQENPEELEAFYGETLEPVVHYDSRYGTELVETLITYLGN"
BASE COUNT          295 a          543 c          503 g          298 t
ORIGIN      
        1 acggccccct gttgggaggc caaagaacca gtttgctttt ggtcatattt tagcacccga
       61 gagctccatt tccgtgctcg ggtttgcgat tttcggccct ggggctcaaa atgatgcgcg
      121 ccggttacac cacgttgcga acggagtttg ccgtgatcct gaaggacttc tcccgcattc
      181 ttctagagga gttggatgcg gactgcccgc tccctggcga cgccagcagg caggtgcgcg
      241 ggctcgctgt cgacgaaccg gtgaaggctt ggatctcacg cgacgaagta gccgtgaccg
      301 cccgcgacac actcgacgag gacttcatgg agggtgtcgc ggccaccggc tctccggcaa
      361 tcgtctggcg tctggatggc gaggcgcagc ctgaggacgt agagagggct gccgcactcg
      421 ggctcggtct gttcgtgctc accccggact tacccctcag gcggctcttt gagatcttct
      481 cccacgacga cggcctactg ctcctctccc acggcgccgc acgcgcgctt ctcgagagcg
      541 cgagcggcgg ttccacggtc gaggacctgg cgggaaggat ctcgggtatg ctccgtcgct
      601 ccgtggtcat agaagatcct gtggggcgtt tgatcgcaag ctcccccggt aaggatcgtc
      661 tgctcacgac gcttgaagag ggggacattc gcgcatcgaa gtcttcgggg atcgacgaga
      721 cgcgccgcga gcgccgcgac cgctacgcga ggcttccaga cggctacctc tccgtacccg
      781 tcgagcgctg gcgggtcgcc gacgacgagt tgttctggac ccccatcggt accggagcac
      841 cggcgggata cctctggatc gacctcgaca gaaccaacct gaagccggaa gacgtggtgc
      901 tcctgtactg ggccagacgc gtcctcgaag ccgaactcgg caaggagcgc atacgcctgg
      961 agacggaact cggcgtcagg ggggacttcg tggacgacct cgttagcggc cactacggct
     1021 cggtcgagct cctcctgcag cgcgcacgct acctcggcgc cgacctcgcc gaaggagccc
     1081 tggtaatggt cgtcgatatt gacgacttcg cccgctacct cgaacgtcga aggccgaagg
     1141 agcccgccat acaggaactg aagcggcgcc ttgccgacgc ggtacgcctg cagacccgcc
     1201 agctcttctc gaatttcctc ctcggtcccc gctcggacaa cgtaatactc ttcctgggtt
     1261 cttctgaagg cgaggatccc gacgagctac cggaaaaggc gcaattgctt gccaaaggag
     1321 tacagcgtta cgtcaaagga ctcctgcccg acctgacggt ctccgtcggg atcggccgct
     1381 acaagaagga ccccgccctc ctcccggacg cctactccga ggcgggagtg gcactcgaga
     1441 tcgggcaccg catccacggc ccttcctccg tctcgacctt cgaggggacg ggcacataca
     1501 agcttctctt cagggtgctc caggagaacc ccgaggagct cgaagccttc tacggcgaga
     1561 ccctcgagcc cgtcgtccac tacgactccc gctacgggac cgagctggtt gagaccctga
     1621 tcacctacct cggcaacga
//
LOCUS       DATAIR010000847         6801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_89828, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000847 DATAIR010000000
VERSION     DATAIR010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89828"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VHM69_20155"
     CDS             <1..1219
                     /locus_tag="VHM69_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2742764.1"
                     /translation="VELILAGDFFDFLQIGAVPEGTDRASLTVARPEYESMFAALRKF
                     REAEGKRVVYLPGNHDAEGWWNPRIQEALKDLGLIDEFAYYYLASIEAGGGRRVIYCE
                     HGNQLDPANTVDDYNDSLDTPLGHHVVMDFTRRLAPYGEISPGLDLAEIKMVYPLVAI
                     PRWIASRYFYDFAGKVAIYLLLPLLVAYTLYRLAAYFLALSQGGRSGLFGGYLELPRI
                     HELFVDIGIFFLLALLVFAVFFLVIRHAVRRTLNTMSPGGTPRYSPAETSENKIRQIL
                     KGEQRPPMNPSLDPTTVDVFVSGHTHLPSLSDLQRPDGRRCALVNSGCWLRQLQPVSP
                     HLKGPPVFVSKFVLTHVRVFVDDDALRVELWEQPKPASQSLTRLERLLSSGRRPPQPS
                     SDSKPRVRASTAV"
     gene            1325..2932
                     /locus_tag="VHM69_20160"
     CDS             1325..2932
                     /locus_tag="VHM69_20160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX2742765.1"
                     /translation="MKGTLRAPVVMAVIVVVVSLSPLLISCSSEGSGGQSGDGRRADE
                     GQRAGSTRTEQTARPGESGGPSKSAHRPEPQAALVPVAHLSSTMENVSMQELSRARNL
                     AVGREFREKAGELTGRSEFQSFDSADAVVDHVSRAPGALGLVPWDEAGPRVKVLSVDG
                     ESLLDPDTAGPERYPLKPKGAAVPDARKLRRVVMAGDIVLDRGQNYMVIQQGMGLDFP
                     LDGGYAAVTSRVPEPSYYSETGVIHEFTAQRTGGAGVVREYLTGADLTLANFENPVIR
                     DAVWHPDATTFTADLRLMPVLDQAGIDGVTLGNNHILDAGTTGLEETLGHLEDAGIAH
                     AGAGMDLSGAREPMVFDLGGTKIGVLSYLGVPSYDWAWATETAPGTAPLLQNVMEEDI
                     KQLRREVDLVAVMPHWGKEYIATPEPGQVDLAHAAIDAGADLVVGGHAHWPKGIEMYE
                     GKPIFYGVGNFLLDQSWSEETSTGIFAEITLYGDDVIQVRPVPFIILDYAQPNFLLPG
                     AGGDRALRKIFAASLGPEFESYETPVR"
     gene            3124..3285
                     /locus_tag="VHM69_20165"
     CDS             3124..3285
                     /locus_tag="VHM69_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742766.1"
                     /translation="MLTDDVGPLARSVEDLRLALRLLAGPDGLDTHVSPVPWRGPERP
                     RMRDLSIAW"
     gene            complement(4243..4593)
                     /locus_tag="VHM69_20170"
     CDS             complement(4243..4593)
                     /locus_tag="VHM69_20170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX2742767.1"
                     /translation="MTWLVGLLLAMVMAMAMSENASASYGEVMADTDGYVPVYSVCYS
                     GTGFLAYYSWYYDGYDTVYGTVYVDDCLLADYGAGPYDRQRIIAHEMGHARGLPHSSN
                     PNSIMYPYYTITGT"
     gene            5070..6146
                     /locus_tag="VHM69_20175"
     CDS             5070..6146
                     /locus_tag="VHM69_20175"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX2742768.1"
                     /translation="MAEDERRRPDSRGVQAYEPRLALRRVQSAVVALAVITGVGVLGY
                     MVFEGWSFTDSLYMTVITVTTVGYREVRVLDVSGQLWTMLLLITGVGTLFYAAVSSVE
                     LAVEGTVRGYFGRRRMKSAIGMLNGHYILCGYGRVGRQVAREFAADEVPFVIVDQDPE
                     VLDECLAEGYLALLGDASDDAVLEEAGVARAGGLIAAVDSDADNVFVVLSARKLNPRL
                     HIVARASSDESASKLEIAGADRTLSPYAVGGRRLASLATQPLVVDFLDVVTRGEKGIE
                     FRLEEFSVPEASTIAERTIGELKIGERTGAIVLAIRTAEGNFDTTPSADDRLRAGDTL
                     IVLGSRGQIGRLERLMRGEEIVDE"
     gene            complement(6164..>6801)
                     /locus_tag="VHM69_20180"
     CDS             complement(6164..>6801)
                     /locus_tag="VHM69_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742769.1"
                     /translation="LGSLAFLLLTPRTGNRAWWLVAPYLLASIPSAILVGKVGSDVNY
                     LLELSAALGLATGALIAWQRKRPRLRVALIALLAIQVLALAQSSRVPSSLQDYVIEQR
                     AEVAQLSRIVASADGPVLTDDYMGLLPLQGRRIYFQPFEMTQLSRDGDWNQRHFVEDI
                     GKQKFPVIMIWSPPFAREIKRDRWTPKMLGEIYDHYERTDRVADMVIYRPR"
BASE COUNT         1275 a         2060 c         2194 g         1272 t
ORIGIN      
        1 cgtcgagctt atcctggccg gggatttctt cgatttcctg cagatagggg ctgtgccaga
       61 ggggacggac agggcctcgc tcacggtggc gcgtcccgag tacgagagta tgttcgccgc
      121 cctgcgaaag ttcagggaag ccgagggaaa gcgggtcgtc tacctgccgg gcaaccatga
      181 cgcggagggc tggtggaacc cacggatcca ggaggccctc aaggatctag ggctcatcga
      241 cgagttcgcc tactattacc tggcttccat agaggccgga ggggggcggc gtgtgatcta
      301 ctgcgagcac ggcaaccagc tcgacccagc gaacacggtc gatgactaca acgacagcct
      361 ggatacgcca ctcggccacc acgtcgtcat ggacttcacc aggcgcctcg cgccatacgg
      421 ggagatatct ccgggcctgg atctcgcgga gataaagatg gtctacccgc tggtggcgat
      481 accgcgctgg atcgctagcc ggtacttcta cgatttcgcc gggaaggtgg cgatatacct
      541 gctcctgccg ctgctcgtgg cttacacgct ctacagactt gccgcctatt tcctcgcgct
      601 ttcgcagggt ggtcggtctg gcctgttcgg aggctacctc gagctgcccc gcatacacga
      661 gctcttcgtc gacatcggta tcttcttcct gctggccttg ctcgtgttcg ccgtcttctt
      721 cctcgttatc aggcacgccg tacgcaggac tctgaacacg atgagcccag gggggacacc
      781 gcgctactcg ccggccgaga cctctgagaa caagatcagg cagatcctga agggagagca
      841 gaggcctccg atgaacccct ctctggaccc tacgaccgtg gacgtcttcg tctcaggtca
      901 cacgcatctg ccttcgctct ccgacctaca gaggccggac ggacgacgct gcgcgctggt
      961 caattcgggg tgctggctgc gccagctgca gccggtctca ccgcacctga aaggtccccc
     1021 ggtcttcgtt tcgaagttcg ttttgaccca cgtgcgggta ttcgtggatg acgacgcgct
     1081 gcgcgtcgaa ctgtgggagc agcccaagcc ggcaagccag agcctgacca ggctagaacg
     1141 cctgctgtcc tcgggacgcc gcccgcccca gccctcctcg gactcgaagc cccgcgtgcg
     1201 ggcgtcgacc gccgtctagc atgtaacttc agctccggct acgtacgggg atgcggaact
     1261 ttgcttgctc tgttggtaaa gtggggcgac tctgaagcgg cctgagatta cgggagggac
     1321 attattgaag gggacgctac gcgcgccggt cgtcatggcg gtgatcgtcg tggtcgtctc
     1381 actctctccg ctgctgatct cttgctcttc tgaggggtcc ggaggtcagt ccggcgacgg
     1441 ccgccgggcc gacgagggcc aacgagccgg ttcgacgcgt acagagcaaa ccgctcgacc
     1501 aggagagtcc ggcggccctt cgaagagcgc acaccgtccc gaaccgcagg cggcactcgt
     1561 acccgtcgcc cacctcagct cgaccatgga gaacgtgagt atgcaagagc tctccagagc
     1621 acggaacctg gccgtcggac gagagtttcg cgagaaggct ggtgagctta cgggccgttc
     1681 ggagttccag agcttcgact cggcggacgc ggttgttgac cacgtgagcc gtgccccggg
     1741 ggcgctcggg ctcgtcccct gggacgaggc gggtcccagg gtcaaggttc tgtctgtgga
     1801 tggcgagtcg ctccttgatc ctgacaccgc cggtccggaa cgttatccac tgaagccgaa
     1861 gggcgcggcg gtccctgacg cccggaagct gcggcgtgtg gtcatggcag gcgacatcgt
     1921 gctcgaccgt ggacagaact acatggtcat ccagcagggc atgggcctgg acttcccgct
     1981 cgacgggggc tacgcggcgg taacgagccg ggtgcccgaa ccgagttact attctgagac
     2041 cggggtcatc cacgagttca cggcccagcg cacaggcgga gccggagtgg tcagagagta
     2101 cctgacgggc gccgacctta cgctggcgaa cttcgagaac cctgtcataa gagacgccgt
     2161 ctggcatccc gacgcgacga ccttcacggc ggatctaagg ttgatgccgg tgctggatca
     2221 ggccggcata gacggcgtca cccttggaaa caaccacatc ctggacgccg ggaccacggg
     2281 gctggaggag accctgggac accttgagga cgccggcatc gcccacgcgg gggccggtat
     2341 ggacctgtcg ggagcccgtg agcccatggt cttcgatctc ggagggacga agataggtgt
     2401 cctctcctat ttgggtgtac cttcctatga ctgggcctgg gccacggaga cggccccagg
     2461 cacggcgccg ctccttcaga acgtcatgga ggaagacata aaacagttgc gacgggaggt
     2521 ggacctcgtc gccgtcatgc cgcactgggg caaagaatac atcgcgaccc cagagccggg
     2581 acaggtggat ctcgcgcacg ccgcgataga cgcaggggca gatctggtcg tcggcggcca
     2641 cgcccactgg cccaagggga tcgagatgta cgagggtaag ccgatcttct acggtgttgg
     2701 caacttcctc ctcgaccagt catggtcgga agagacctcg acgggcatct tcgcggagat
     2761 caccctgtac ggggacgatg tgatccaggt ccgccccgtc cccttcataa tccttgacta
     2821 cgcccagccc aacttccttt tgccgggggc tggcggggac agggctttgc gcaagatctt
     2881 cgccgcctct ctcgggccag agttcgagag ctacgagacc ccggtccggt gaaatagccg
     2941 cagcctgcct ccgcaacccc ggaggaagca ggaccacggg accaggcgct tccacgacct
     3001 gcgccggtac attagagaac ccgctcgctc tgccgtctcc gaaatgtctc aggggctcgt
     3061 caggcgtatg atgccggcgt tgcaatcgcg tatgaaggga aagcgggttg acggaaggag
     3121 gccatgttga ctgatgatgt cggtccattg gctcggagtg tcgaggacct gcgcctcgcg
     3181 ctacgtctcc tcgccggacc ggacggtctc gacacacacg tttcgccggt gccctggcgc
     3241 ggacccgaac ggcccagaat gcgcgacctg agcatcgcgt ggtagtccga gttccccgat
     3301 agcagcacac aggatgagat ccgtgccgcg gtggaggacg tggtccagaa actcacagga
     3361 gagggtgcaa tagtcgaaaa gagcatgcca gaggttgatc ttatgaacca gtactccgta
     3421 acgttctcgg aagagactac cttgttcgca gagcaggcga acggtgcaga aagctccctg
     3481 gaatcgtacc tgatggccat ggaaggccgt gacgaagtga tgagagcctg ggaggcgttc
     3541 tttgctgact gggatgtacg gccgaacggc acggcgagga actcaacgag ctatcacaag
     3601 agtgtcccta ttccctctcg gctgtctcgg gctgcgtcat accggccgga gtcgacagcc
     3661 agggactacc tttcgggccg cagatcctgg cccgccgctg ggatgacgag cggcttcagg
     3721 agatcgcgta gttcgtgtcg gaactgacgg gtggattccg acgactgcag ggccattgag
     3781 gcaggaagct ttcttctcga agaagagttc gggtgcttct ccgtaagcgg cggcggcttc
     3841 tacctgccct gggagcgcac gctttctgcg gcggcagagc ccgatggttg tcggggtatg
     3901 caagcggcaa ggtgaagttg cgcccgaagc gcgtcactgc gctacccatc gtcgggtttc
     3961 aaggtaaggt ccgccgtgcg cttggtgagg tgtgatccgc aggcgtggac gcaaggtcgt
     4021 acagattgcg ctctcgcagt accgcctttc taccctaccg ttgcaagtct ccgatcagaa
     4081 caggtgttga tgaagcgcct atgaacatag agtaggatcg tatatccgac acccgaccct
     4141 ggcgcgggta ggggagtaac ccaggaaacg catttccgag agagctgctt tctcggaaat
     4201 gcgtcccggg cgagtcgctg gccggcggct acctgtatcc gcttacgtgc ctgtaatagt
     4261 gtagtacggg tacatgatgc tgttaggatt ggagctgtgg ggtagcccgc gagcgtgtcc
     4321 catctcgtgg gctatgatcc gttgtcggtc gtagggaccc gcaccgtaat cggccaagag
     4381 gcagtcgtcg acgtagacgg tgccgtagac tgtgtcgtag ccgtcatagt accaactgta
     4441 ataggcaagg aagccggtcc ccgagtagca gacactatag acgggcacat atccgtcggt
     4501 gtctgccata acctctccgt aggaagcgga ggcgttctca gacatagcca ttgccattac
     4561 catcgccagc aacaggccca ccaaccacgt catgaccagc cttcgagtcg gcgcaaagcc
     4621 cactactaac cttctgacca agagatcccc ctcttcctcg tcgtgggcct ggtttcctcc
     4681 agagcacctt ccgagcggta tttgtccaca accctaccct aatcgctggc gcagtgcaat
     4741 gttttgtact ggctgacgca gcgaaatgcc cagattttgt gccacttgcc gggctatccc
     4801 caagcgaact acggaccgtt tcacccgggc ctcgtagtca tcgcgacaga agatgtccgg
     4861 gaaccctcac gggggaccaa ttttctcgca atcaggcgac gaatcgccac gttgacaaga
     4921 gctcctacgc tctccggctc ctgcgcgtca caggccggat gagttgagca acccggggca
     4981 gcccggacgt acgtacttgc gtgttggagg cgacgaatta aaggctgaag cgctggcgtt
     5041 gtgagcagcc gaggcggata ataccgctcg tggcggagga cgagcgcagg agacccgaca
     5101 gccggggcgt tcaggcgtac gagccaaggc tggcgctgcg gcgggtgcag agcgctgttg
     5161 tggctctcgc ggtcataacc ggcgtaggcg tgttggggta catggtgttc gagggatgga
     5221 gcttcaccga ctccttgtac atgaccgtga tcaccgtgac caccgttggg tacagggagg
     5281 tccgcgtgct cgacgtatcg ggacagctat ggaccatgct tctcctgatt acgggggttg
     5341 gcacgctgtt ctacgcggcc gtatcctccg tggagctcgc ggtcgaaggg acggtgcgag
     5401 gctactttgg aaggagaagg atgaagtcgg cgatcggcat gctaaacggc cactacatcc
     5461 tgtgcggcta cggacgggtc ggaaggcagg tcgcccgcga gttcgcggct gacgaagtgc
     5521 ccttcgtgat cgtcgaccag gatcctgagg tcctggatga gtgcctggct gaaggctatc
     5581 tcgcgttgct gggcgacgct tcggacgacg ccgttctcga ggaggccggt gtagcgcgcg
     5641 caggcgggct tatcgccgcc gtcgactccg acgccgataa cgtcttcgtt gtgctctcgg
     5701 cccgcaagct caaccccagg ctgcacatcg tcgcccgcgc ctcttccgac gaatcggcgt
     5761 ccaaattgga gatagcgggc gcggaccgta ctctttcgcc gtacgccgta ggcggcaggc
     5821 gcctcgcctc gctcgcaacg cagccactcg tcgtggactt cctggacgtc gtcacccgtg
     5881 gcgagaaagg catcgagttc cgcctcgagg agttctcggt gcctgaggct tccaccatcg
     5941 ccgagcgcac catcggggag ctcaaaatag gggagaggac gggcgccata gtactcgcca
     6001 tccgcaccgc agagggcaac ttcgacacga cgcctagcgc cgatgatcgt ctgcgcgcag
     6061 gcgacacgct catcgtgcta gggagtcgcg gtcagattgg gcggctggag cggctcatgc
     6121 gcggcgagga gattgtggac gagtagggtc ggtaccgccc ctactacctc gggcggtaga
     6181 tgaccatatc tgcgacacgg tccgtcctct cgtagtgatc gtatatctcc ccgagcatct
     6241 ttggggtcca cctgtcgcgt ttgatctccc tcgcgaaggg aggactccag atcatgatca
     6301 cgggaaactt ctgcttaccg atgtcctcga cgaaatgtct ctggttccaa tcgccgtcac
     6361 gggagagctg ggtcatctcg aagggctgga agtagatcct gcgtccctgc agcggcagga
     6421 gtcccatgta gtcgtccgtc agcacgggcc cgtccgcgct cgccacgatc ctggacagtt
     6481 gtgcgacctc ggcccgctgc tcgatgacgt agtcctgcaa actcgaaggg acccgcgacg
     6541 attgggctag tgccaggacc tggatggcta ggagcgcgat cagggcgacc cgcaaacgtg
     6601 gacgcttgcg ctgccaggcg atcaaagccc ccgtcgcgag gccgagcgcc gctgacaatt
     6661 ccagcagata gttcacgtcc gagcccacct tgccgacgag tatcgcggag gggatgctgg
     6721 cgagcaggta gggtgccacg agccaccacg cccggttccc agtccgcggg gtcagcagga
     6781 ggaacgcgag ggagccgagc a
//
LOCUS       DATAIR010000848         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U6889 k141_91739, whole
            genome shotgun sequence.
ACCESSION   DATAIR010000848 DATAIR010000000
VERSION     DATAIR010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955507
            Sequence Read Archive: SRR12721349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 03:10:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,039
            CDSs (total)                      :: 3,989
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 4 of 12
            Pseudo Genes (incomplete)         :: 8 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91739"
                     /isolate="SMAG_U6889"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.14 N 105.42 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VHM69_20185"
     CDS             <1..699
                     /locus_tag="VHM69_20185"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2742770.1"
                     /translation="KERDLPPLDDDFVKEASEFETLDELRTAVREQLEAALEQRVEGE
                     FRGRVLDAVAERAEVEVPDVMIHEKAHEMVESFERSIRAQGMEPEQYYQLAGASHHDM
                     EERVRPDAEDTVKKELVLDAVAVKEGIEADDASVMHEIGHMAEGGERSADQIADMMRA
                     NGTYTLLKEEIARQKALDYIVENAVAVEMPEDVEPAEKEPTDEESAGEDSDEAVAVAE
                     AGDGTETSEGDEKQ"
     gene            696..1298
                     /gene="clpP"
                     /locus_tag="VHM69_20190"
     CDS             696..1298
                     /gene="clpP"
                     /locus_tag="VHM69_20190"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX2742771.1"
                     /translation="MSYYDPQGVIPYVIEQSPRGERAMDIYSRLLKDRIIFLGTPVDD
                     QVANAIMAQLLHLESEDPEQDINLYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMA
                     ASMGAFLLAAGAKGKRNALPNTRILLHQPSVGGLAGQASDVEIHAKELILTKQRLNQI
                     LAENTGQEYDKIERDTDRDYIMGAEEAVEYGVIDNIVRQH"
     gene            1353..>1997
                     /gene="clpX"
                     /locus_tag="VHM69_20195"
     CDS             1353..>1997
                     /gene="clpX"
                     /locus_tag="VHM69_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX2742772.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQGDAKKVLAVAVYNHYKRISMSPDASDG
                     TELQKSNILLLGPTGSGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKILEGTVA"
BASE COUNT          457 a          575 c          680 g          285 t
ORIGIN      
        1 aaagagcgag acttgcctcc cctcgacgac gattttgtca aggaggcgag cgagttcgag
       61 acgctggacg aactgcggac tgccgtccgg gagcagctag aggcggcgtt agagcagcgt
      121 gtcgagggcg agttccgtgg gcgggtgcta gatgccgtgg ccgagcgggc cgaggtcgag
      181 gtgccggacg tgatgatcca cgaaaaggcg cacgagatgg tcgagagctt cgagcgcagc
      241 atccgggcgc aggggatgga gccggagcag tactatcagc tcgccggagc cagccaccac
      301 gacatggaag agcgtgtgcg gcccgacgcg gaggacacgg tcaagaaaga gttggtgctc
      361 gacgcggttg cggtgaagga gggcatcgag gccgacgatg cgtcggtgat gcatgagatc
      421 gggcacatgg ccgaaggcgg cgagcgctcg gcggatcaga tcgccgatat gatgcgcgcc
      481 aacgggacct acactctcct gaaggaggag atagcccggc agaaggctct tgactacatc
      541 gtcgagaacg ccgtcgccgt ggagatgccg gaggacgtgg agccggcgga gaaagaaccg
      601 acagacgaag aatcggcggg agaggattcc gacgaggcgg ttgccgtcgc ggaggctggc
      661 gacggtacgg agacgagcga aggagacgaa aagcaatgag ctactacgat ccgcagggcg
      721 taatcccgta cgtgatagag cagagccccc gtggcgagcg ggcgatggac atctactcgc
      781 ggctcctcaa ggaccgcatt atcttcctcg gtacgccggt ggacgatcag gtagccaacg
      841 ccatcatggc gcagctactg cacctggaga gcgaggaccc ggagcaggac atcaacctgt
      901 acataaactc gccgggcggt agcgtcacgg ccggactggc gatctacgac acgatgcagt
      961 tcgtgaagcc ggacatctcg acgacggcgc tggggatggc cgcctcgatg ggggctttcc
     1021 tgcttgcggc cggcgcgaag ggcaagagga acgcgctgcc aaacacccgg atactgttgc
     1081 accagccgag tgttggcggg ctcgcgggac aggcctcgga cgtggagatc cacgccaagg
     1141 agctaatact gaccaagcag cgcctgaatc agatcctcgc cgagaacacc gggcaggagt
     1201 acgacaagat agagcgcgac accgaccggg actacataat gggcgccgag gaggctgtcg
     1261 agtacggcgt catcgacaac atcgttcggc agcactagga tgccggcagg aggagacgtg
     1321 caagcggagc gaggcttggg aggccgaaac tcatgaggga cccgtcggac cagttgcagt
     1381 gctctttctg cgggaagagc caacgccagg tcaggaagct tatagccgga cccggcgtct
     1441 acatctgcga cgagtgcatc gaactctgca acgagattat cgacgaagag ttttccggcc
     1501 cggaggtctt gaaagacgac gacctgccga agccccgcga gatcaaccgc atcctcaacg
     1561 agtacgtgat cggccagggt gacgcgaaga aggtcctcgc cgtcgcggta tacaaccact
     1621 acaagcgcat ctcgatgagc cccgacgcct ccgacggcac cgagcttcag aagtcgaaca
     1681 tactgctgct cggtcccacg gggtcaggga agaccctgct cgcgcagacg ctcgccaaga
     1741 tcctcaacgt ccccttcgca atcgccgacg cgacggcgct caccgaggcc ggctacgtgg
     1801 gcgaagacgt ggagaacata ctcctcaagc tcattcaggc cgccgacttc gacgtgaaga
     1861 aggccgagac gggcattatc tacatcgacg agatcgacaa gattgcccgc aagtccgaca
     1921 acccctcgat cacccgcgac gtgtcgggcg agggcgtgca gcaggcgctc ctcaagatac
     1981 tcgaaggcac cgtggcg
//
